LOCUS       DAOQMB010000001        35839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_356_length_35839_cov_14.422619, whole genome shotgun sequence.
ACCESSION   DAOQMB010000001 DAOQMB010000000
VERSION     DAOQMB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 35839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35839
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_356_length_35839_cov_14.422619"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PLY05_00005"
     CDS             <1..165
                     /locus_tag="PLY05_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011280630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNC01715.1"
                     /translation="QDGLSDAEIDALIQARADAKKAKDFAQSDKIRADLLAKGIILED
                     SRQGTTWRRG"
     gene            277..352
                     /locus_tag="PLY05_00010"
     tRNA            277..352
                     /locus_tag="PLY05_00010"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Gly,seq:gcc)
     gene            419..1252
                     /gene="murI"
                     /locus_tag="PLY05_00015"
     CDS             419..1252
                     /gene="murI"
                     /locus_tag="PLY05_00015"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNC01716.1"
                     /translation="MLSASSAPIGVFDSGYGGLTVLKELLQHLPEYDYLYLGDNARTP
                     YGTRSYQTVYEFTLECVRYLFDAGCPLVILACNTASAKALRTIQQHDLPLLDASKRVL
                     GVIRPSVEQVGLLTKTGHVGILGTTGTVNSESYRLEINKLFPEIQVFQQACPMWVPLV
                     ENGEYQDEGADFFIKRDLGLLFAQSEKIDTIILGCTHYPLLINKIRQYLPSHIQVLSQ
                     GEVVAHSLKDYLQRHTEMAKRCSQFTQRQFLTTDNPETFSQLASLFLGQSIDAQKISL
                     S"
     gene            1620..2681
                     /locus_tag="PLY05_00020"
     CDS             1620..2681
                     /locus_tag="PLY05_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HNC01717.1"
                     /translation="MTDKRYQVFISATYMDLREERGMLLQTLPSLGCLPSTFEAHTQS
                     LSTMVNIRRRIDECDYYLLIVGSRYGSLMPSGVSYTHMEYVYAATKQKPMLVLMHESP
                     ETRPTEFQEQSIEGKLKFNDFRKVLQRERDNIVYWRDVKDLSHALHNAIPALIRKYPT
                     VGWSRVIKGASTGATSTVSNSALEKECEALKRRVKELEAEKEKWLSAGVNSPETLAKG
                     EDLFDIQYKCKAYAGGNCQDVFGKTRLSWNELLVSFGSNLLQAQTEEFMAAKLNERLQ
                     KNALAEIQSEYPKTHAVVDVQITPATFNAIKMQFRVLGYIQRVSRENDLKQWWQMTAI
                     GERMLASIMSVRRTASRLA"
     gene            2744..>3237
                     /locus_tag="PLY05_00025"
     CDS             2744..>3237
                     /locus_tag="PLY05_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC01718.1"
                     /translation="MRQWNSWGDSETKMAINAKAAQFLQELIGQSTALADATLAEVMA
                     KVPVTRLPKHPLISIDAEDRVRHARGQSLPDWLAWRSGEIPVFPDGVAFPTNSQQVAE
                     LLALAKTHNWVVIPYGGGTSVVGHINPIADERPILTLSLARLNQLIALDEASQLATIG
                     AGANG"
     assembly_gap    3238..3337
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3338..4168
                     /locus_tag="PLY05_00030"
     CDS             <3338..4168
                     /locus_tag="PLY05_00030"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNC01719.1"
                     /translation="EAVVRVSRTPNKEVFCVYFVPDWAKAKAAVRELVQQKIPLSMVR
                     LSNGEETRTQLILAGHALAIAGLEKVLNWRGVGAGKCMLTIGFTGSEQRIKFARNEVT
                     RILKEFDCVATGQVLGKRWAQNRFRAPYLREALWYLGYAVDTLETAVDWHKVTPYMTD
                     VEQALRSALQDENEHVLAFSHLSHVYGQGSSIYTTYVYRVAGNYQQTYARWQKLKAAA
                     SAQIIKHGGTISHQHGVGQDHAPYLPAEKGVLGMSVLKTLASHFDVDKRLNPHKLLED
                     "
     gene            4355..5929
                     /locus_tag="PLY05_00035"
     CDS             4355..5929
                     /locus_tag="PLY05_00035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNC01720.1"
                     /translation="MLNRDVLWQELPQTEWDIIVVGGGITGAGVAREAARAGAKVLLI
                     DQKDYAFGTSSRSSKMVHGGLRYIAMGDIKLTRHSLVERERLLREAPGLVDRMSYFFA
                     HYQGGGIKSNRWAFAILLMAYDILAGIKDRSYFNAKKFLARVPNYRSDKLLGASRYTD
                     AVTDDTRLVIRVLDEACKDGAVTVNYVKAEKTIITNDQVTALVVRDELSQETATLKAK
                     VVVNATGAWVNRLRQEVATETVSVRPQRGSHLVVSAERLPLNDALILFHPDDNRAMFI
                     YTWEGRTVIGTTDLDHKDNLDDEAVITAEEVKYLLRAANHEFPQAKLTPNDVIATFSG
                     VRPIVSSGKGLDPSKERRDHAVWADKGVISVSGGKLTTFRQIAQDVLTAAESWLPTIK
                     QRDQKASVFSKPEGHISSPLSPDQYRRFVGKYGYLAQRFLAEMPAQELKLIPETQTMW
                     AEVRWALRYEQVVHLDDLMLRRTRLGLLLVKGGAAHFDTIKQMALSEGWTEERWVSEQ
                     ARYSQIWQAYYSLPKL"
     gene            5972..7498
                     /locus_tag="PLY05_00040"
     CDS             5972..7498
                     /locus_tag="PLY05_00040"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNC01721.1"
                     /translation="MNKARFLSIDCGTQSLRAFIFDAKGELLARNQIHFEPPYQSPKQ
                     GFAEQDANYYWHQLVTACQSLWQQGIDPNTVTAASVTTQRGTTVIVDERGQPLYPAVL
                     WLDERQSEKLPALPVYWQKLHQVSGHQQTILKLRKRAPANWFISHRPELWAKTYKFLL
                     LSGYLNYKLTGEYSDSIPSQVGYIPFDYKKHQWHSRWDWRWQALGLTAQKLPKLIPAG
                     KLIGQLTVAAAHELGLPAGLAIVAAGADKACEVLGAGCVNATQAALSFGTTATYNTVT
                     SRYLSLNPPMPPYPAVLPKQYCLETQIPQGFALVKYFKEQIAANEIAEAQQGRDWLAV
                     LEDWLNATPAGANGLMWQPSLSVQASGESVSKGALLGLSTQHTKADWYRALVEGLLFA
                     LKQGQEHVEQHTKQKIAQLFAAGGGSQSNAVMQIAADIFNMPISKPHTSETSGLGAAM
                     CAAVAMGIYPDLNQAVRAMSRTEKVFYPNTINARFYQQRYQYVYKPWVQSLIQLYATN
                     "
     gene            7565..8401
                     /locus_tag="PLY05_00045"
     CDS             7565..8401
                     /locus_tag="PLY05_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC01722.1"
                     /translation="MKWRQWLVASSFAVLTACTSSTHEGATGIQRKQFLLLPAEQVNA
                     MALQSYRGELSQAKQKGALNTDKANLERIRKIADRLIPQVATFRPDALNWAWEVNLES
                     RNELNAYCAPGGKIMFFTGIINNLKLTDDEIAAIMGHEIAHALREHGRERISQAYAQQ
                     AGMAILAMVTDMSQKQAAILQTATHLGLTLPHGRGQESEADVMGLELLARAGYDPKAS
                     VTLWQKMASANKNSPPAFLSTHPSSAQRINELNSKITVVMPLYEAAKRNPPASKTIVP
                     RT"
     gene            8414..9178
                     /locus_tag="PLY05_00050"
     CDS             8414..9178
                     /locus_tag="PLY05_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC01723.1"
                     /translation="MFHSLNIFSSVVASTVRGWRGSKGSKTTQQPVQLPILFDSEDNA
                     ECRLVREALTELNLDVLIYPCPQGADRFAGELQRYGGANASIPYLVDPNTNVKLEGAE
                     AINAYLFQQYKQQEIPKNLDATQLNLFNSRLASFARLRITALQAKPAKSAAQPLILYS
                     FESSPYSRPVREKLCELQLPYRLINLGKQQLADVGPATFRFHLGEYHPIPNSKRAELL
                     TTKGRVQVPFLIDPNNNIEMFESKDILEYLDKMYAL"
     gene            complement(9239..9820)
                     /locus_tag="PLY05_00055"
     CDS             complement(9239..9820)
                     /locus_tag="PLY05_00055"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HNC01724.1"
                     /translation="MALFTKMYDMALRWSRHPHAERYLAALSFTESSFFPIPPDVMLA
                     PMSMTKPKKALRFALITTIASVLGGVFGYAIGAMFYHQFLPLFEQLGWSSHFATIDEW
                     FSKWGVLAVIIAGFTPIPYKVFTIAAGFASMALLPFVLASCVGRGARFFLVAGLMAWG
                     GEKMEHRVRGIVEWLGWGLILLIALWVGYKSLT"
     gene            complement(10109..11155)
                     /locus_tag="PLY05_00060"
     CDS             complement(10109..11155)
                     /locus_tag="PLY05_00060"
                     /inference="COORDINATES: protein motif:HMM:NF039651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC01725.1"
                     /translation="MVRLSTTKYVTGLALISSLYTANVFAAPLNPLSKPLNMTFCVYD
                     VAGNGGDLYRYAKDLSLFAQKWNVNAKIRAYTDERVIAEDFKANQCDGIAVSTLRAKQ
                     FNNFVGSIDSIGSAPSYRHIKELVSTFNHPKIAPLTIQGDYQVVTVMPLGASYVMVNN
                     RHIDSVEKATGKKVAVIETDKAHSKLVHLLGAQAINTDFYNFANLFNNGQVDIITAPA
                     IAFKPLELSKGLSYYGGIYRLPIMQMTGTVLINRTNLAKQIPDLDLRLEQIRQYALGE
                     MDKTIAYIEKTEKAIPQQYWLDLSDAEKTRYITMMREARNHLTKEGYYDERMMNLMKK
                     VRCKISPSDSECGV"
     gene            11396..12787
                     /gene="sthA"
                     /locus_tag="PLY05_00065"
     CDS             11396..12787
                     /gene="sthA"
                     /locus_tag="PLY05_00065"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017387960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HNC01726.1"
                     /translation="MSYDYDVIVVGAGPAGEGAAMKLTKEGKKVAVIDARPQVGGNCT
                     HVGTIPSKALRQTVFNIMRYQRDSMFQRVGELRNVPLSQVLARAHKIIEQQVEVHTRF
                     YDRNRVDMYFGQAQFVDEHTLKVTTIEGGHEILTFENAVLATGSRPYHPTDIDFSHPR
                     IFDSDKILTLNYPVHKLIIYGAGVIGCEYASIFVGLGYKVDLINTQQQLLSYLDDEIA
                     DALSYYLRELGVLIRHQEQYDYVETFDDYVVLHLKSGKKIKADAILWCNGRTGNTDNL
                     ALDQIGVNANSRGQIAVDKQYRTSISNIFAAGDVIGWPSLASAAYDQGRCAAAHILGL
                     KDTPHVEDVPTGIYTIPEISSLGKTEKQLTEEQVPYEVGQAFFRHLARAQITGETVGM
                     LKILFHRETLEILGIHCFGNNAAEIVHIGQAIMQQKNGGNTLQYFANTTFNYPTMAEA
                     YRVAALNGLNRLF"
     gene            complement(12836..13882)
                     /locus_tag="PLY05_00070"
     CDS             complement(12836..13882)
                     /locus_tag="PLY05_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC01727.1"
                     /translation="MKKSLVLASTLFSTMLSSWAIAAPANAEFPQTVKMKFCIFDIAG
                     TKGDAYTYAKDLVLEAKKWNLDVDLKVYTDERVAAEDFKAGQCEGVAISTMRAKQFNF
                     FIGSVDSPGSLPTFKHVRELVNTLANPKLNSLTISGPYQVAALIPLGGAYVFVNDKKI
                     NSVEKAAGKKVAVLDFDKAQAKIVQKLGAQPVASDITNFAGKFNNGQVDIIGAPAVAF
                     RPLELYRGLGENGAIFRFPLVQVTASILINREKYKDIPDFDQRIIKMRQFAASKMDMV
                     EKLVNKSESDIPAKYWLDLTPADKEKYTTLMREARINMTKEGFYDPKMMVLLKKIRCK
                     FEPTLAECSTKDET"
     gene            complement(14011..15141)
                     /locus_tag="PLY05_00075"
     CDS             complement(14011..15141)
                     /locus_tag="PLY05_00075"
                     /inference="COORDINATES: protein motif:HMM:NF039651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC01728.1"
                     /translation="MAFSSIVTFFNRHVSLLGKTLVLGLLSTSLYAGDILSIANDLNP
                     NEDGLPPPLEKPLDVSVCFFDMQGKNGDFYSKAKDLALIAKRWNVIANIKVFTDERVA
                     AENFKAGQCDAAGISTMRARQFNLFMGSIEAVGGITTYEQLRLLLRILLDPKISQLTI
                     TEPYQILGVLPVGGTYIHVRDRMINSVEKAAGKKIAVLDWDKSQTEIVNKIGAHPVPS
                     NLSNFASKFNNGQVDIIVAPALVYRPFELNRGLGETGGIYRLPLTQMTASFVINRAKI
                     KQKLPDLDEKLVAFRTIADKFIDQILTQTYQWIDREEAAIPSKYWLTIDPDNEQKYWN
                     MLKTARIEMTKQGYYDPKMISILKRVRCKTNPERAECSQTDE"
     gene            15746..16672
                     /locus_tag="PLY05_00080"
     CDS             15746..16672
                     /locus_tag="PLY05_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HNC01729.1"
                     /translation="MKTQQKIFASLVVALSTSLVTQGAIASTYSQTKYPIVLAHGMFG
                     FKSLLGGVVDYWYQIPSDLQANGAKVYVTQVAALESSEVRGEQFLKQVQDVLAITGAA
                     KVNLMGHSHGGHSVRYVAGLMPSKVASVTAIGSPVKGSPVADLVTQTGVLSPIAYGAL
                     NALAGIIELFNGAGSFNQSAKNSIASLSTKGSAAFTVKFPQAVPTTACGQGAATVNGV
                     KYYSWSGTKRLTNVLDPTDALVGATGLFISTANDGLVSQCSSHLGVVLRDDYSMNHLD
                     EVNLMFGLRDIFSTDPKSVYRSHANRLKLAGL"
     assembly_gap    16712..16811
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16812..18388)
                     /locus_tag="PLY05_00085"
     CDS             complement(<16812..18388)
                     /locus_tag="PLY05_00085"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HNC01730.1"
                     /translation="MATYQSALEHVRAVKAKLGNTWGAIHPEDAARMIIQNRFPTGLE
                     IAKYTAAIMRKDMAAYDADSSKYTQSLGCWHGFVAQQKMIANKKYFGTTERKYIYLSG
                     WMVAALRSEFGPLPDQSMHEKTAVPKLIEEIYTFLRQADAKELNDLFRALQKAEDAGD
                     AAKAAEVTAKIDNFESHVVPIIADIDAGFGNEEATYLLAKKIIQAGACAIQIENQVSD
                     AKQCGHQAGKVTVPHEDFLSKINAVRFAFLELGVDEGVIVARTDSEGADLTQKIPVVK
                     KAGDIASQYISYLDTKEIDISEAQDDEVLIKRNGKLHRPTRLASGLFQFREGTQIDRV
                     VLDCISSLQNGADMLWIETPTPNVGEIAHMVNRVKEVIPNAKLVYNNSPSFNWTLNFR
                     QQAYDRWVKEGKDVSAYDRAKLMDAKYDNTELSADADEKIRTFQADAAREAGVFHHLI
                     TLPTYHTTALHMHELSKGYFGEMGMLAYVAGVQRKEIREGVSCVKHQAMAGSDIGDDH
                     KEIFSGDNALKAGGTANT"
     assembly_gap    18532..18631
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <18632..19359
                     /locus_tag="PLY05_00090"
     CDS             <18632..19359
                     /locus_tag="PLY05_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC01731.1"
                     /translation="SSARVFRIMASDYAEATLIPALVKAMRSEAPNVVLDFLTPSDVS
                     YRDMEQGKVDLAINRFNEIPQSFHQVLVWRDSFSCLLHCDSPIVDHFNLKTYLEAQHI
                     WVSKTGMGVGFGVNPDKSGGLGWIDQALARLGKKRKISIFTRHYQMPALLAANADLIA
                     TLPSRVAKLQATNPRLVIKEPPFYVPEFELKMAWCPLLHHHPAHRWLRQLILFVARQL
                     IEDERKQMERQIALQQMMGRYED"
     gene            19408..20043
                     /gene="ccmA"
                     /locus_tag="PLY05_00095"
     CDS             19408..20043
                     /gene="ccmA"
                     /locus_tag="PLY05_00095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HNC01732.1"
                     /translation="MLTVSDLCAVRDDRVLFAKLDFQLQAGEIMQVVGANGTGKTTLL
                     NGLMGLAPFESGAIYWQGENIQDNPSVFKQNVAFVGHLLGLKPLLTAVENLQFLAQLH
                     QKFISLADIEQALKKVGLYGYEDVPIARLSAGQKRRVALARLFLQQAKLWVLDEPFTA
                     IDLAGVQALEQWLVDHAQQGGMVLLTTHHVFEPDFPLKHLDLAQYRVQEGY"
     gene            20046..20717
                     /gene="ccmB"
                     /locus_tag="PLY05_00100"
     CDS             20046..20717
                     /gene="ccmB"
                     /locus_tag="PLY05_00100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003441898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNC01733.1"
                     /translation="MWPSLWAVIQRDLKLVSRQGGEWLNPLSFFLMVITLFPLAISST
                     PTKLAAMAGGVVWIAALLAVLLSMDSLFRQDYDDGSLEQLLVSPAPLPLVVLAKVIAH
                     WLQTGACLTVLSPIAALLLHLPNDAAWALFITLLIGTPTLSLVSSIGAALTVSLKRGG
                     VLIPLLTLPLHIPVLIFATAAVQAALNGLPIGGYLALLGAFLVLALTLSPLAVAAALR
                     LAVGG"
     gene            20721..21503
                     /locus_tag="PLY05_00105"
     CDS             20721..21503
                     /locus_tag="PLY05_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HNC01734.1"
                     /translation="MFFLKRWWQLFEQTVSPKYFYSISERFIPWLSVLAGLLIAVGTV
                     WGLAFAPTDYLQGNSYRIIFIHVPSASLAMSAYFLLGICGVISLVWKVKTAEMVARAV
                     APVGAVFCFLALMTGAIWGKPTWGTYWVWDARLTSMLILLFLYMGVIALYNAYESSST
                     GAKAAAILSVVGVINLPIIKYSVVWWNTLHQGATFTVTQKPKMPPEMWMPLLVMILGF
                     YSLFGLLTILRTRNVILERERRSQWVKELVKTEAGRGGQRGV"
     gene            21493..21675
                     /gene="ccmD"
                     /locus_tag="PLY05_00110"
     CDS             21493..21675
                     /gene="ccmD"
                     /locus_tag="PLY05_00110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HNC01735.1"
                     /translation="MAFNSLAEFWAMGNHGIYVWSCYALTFSAILYLVWQAKAQRQRF
                     FKEQIQQLKRGQQHES"
     gene            21665..22102
                     /gene="ccmE"
                     /locus_tag="PLY05_00115"
     CDS             21665..22102
                     /gene="ccmE"
                     /locus_tag="PLY05_00115"
                     /inference="COORDINATES: protein motif:HMM:NF009731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNC01736.1"
                     /translation="MNPKRQRKLVLIISLVLGIGVAIGLAMYAMRQNINLFYTPSQLA
                     NGEAPDGQRIQAGGLVVKGTVVRDPSSLAVTFMISDLKKQVPVTYTGILPDLFKEGQG
                     IVANGYFKNGRVEAEEVLAKHDENYMPPQVKQAIEEAGHPTGK"
     gene            22108..23346
                     /locus_tag="PLY05_00120"
     CDS             22108..23346
                     /locus_tag="PLY05_00120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC01737.1"
                     /translation="MRQNYLFYLRCLAILGQSITIGLVHFFLQLSLPLLPLILTLSVL
                     VAANIHLAYYLKTHPNKDTDDFIAIQLTIDMLALATLLYFTGGAQNPFIFLFLLPLVI
                     GAAMLPVGYTMALFLLSVFLFTRLMFDYVPLENISQKLPLAINGYVLAMWICFVLSAV
                     LIVSFIAYLAYQVKHSQQKMAKIQEIQHRQEQILALGTLAAGTAHELGTPLATITVLA
                     HELKSEVAESLKPDMELLCQQVNQCKQILTQMTQRIEQAKNQEFDLLSLSKLLELVLD
                     KFQLLRPTIQIKHADFGGLTDILVATDATLEQAILNLLNNAADASPDYVELTAYIDSS
                     RVYINVIDKGLGMSDEILTSLGQNRISTKGQQGMGIGLLLANATLERLGGEMNFTHRQ
                     GGGVVAQIVLPIYQRVIHVG"
     gene            23336..23875
                     /locus_tag="PLY05_00125"
     CDS             23336..23875
                     /locus_tag="PLY05_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC01738.1"
                     /translation="MLDNLPLLLIVDDDHIFRQVLAKAMLKRGYEVIQAESVEQAIEL
                     IALQTPEYAVIDLKMNGASGLELVKYLHDLDEQTRMVVLTGYASIATAVEAIKLGATH
                     YLAKPANADEIMAAFLRIEGDSALLVNDNPLSVDRLEWEHIQRVLQEHEGNISATARA
                     LNMHRRTLQRKLAKYPVKF"
     gene            complement(23865..24488)
                     /gene="nth"
                     /locus_tag="PLY05_00130"
     CDS             complement(23865..24488)
                     /gene="nth"
                     /locus_tag="PLY05_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010589511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNC01739.1"
                     /translation="MNASTINEIFRRLQQQNPKPTTELVYNSPFELLIAVILSAQATD
                     VSVNKATAKLYPIANTPQKILALGLDGLKSYIKTIGLYNSKAANIMTTCQILIDKHQG
                     QVPHQREDLEALAGVGRKTANVVLNTAFGEPTIAVDTHIFRVANRLGLANSKTVDGVE
                     KQLLANVPNQYKLDAHHWLILHGRYVCTARKPQCETCLLADLCPSKT"
     gene            complement(24488..25198)
                     /locus_tag="PLY05_00135"
     CDS             complement(24488..25198)
                     /locus_tag="PLY05_00135"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNC01740.1"
                     /translation="MSETSYRQIAVDGLWRNNTATVQLLGLCPLLACSNSVVNALSLG
                     LATTLALASSNAAVSLLRHHTPANLRLPIFVMIIASLVSCIELLMHAFTYQLYQALGI
                     FIPLIVTNCALLGRAEAFASKHNTRFAAFDGLMVGLGFTVVLVLLGAMRELLGNGTLF
                     ANMHLLLGNIAQSWTIKIADYTQFLFFILPPSAFICLGFIIAGKNVIDERLKQRASQQ
                     AKEVVAGSKRVRTTGHIS"
     gene            complement(25195..25863)
                     /gene="rsxG"
                     /locus_tag="PLY05_00140"
     CDS             complement(25195..25863)
                     /gene="rsxG"
                     /locus_tag="PLY05_00140"
                     /inference="COORDINATES: protein motif:HMM:NF002519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HNC01741.1"
                     /translation="MSIREQSLKSGLILAVFAAITTALIAIVNTMTSDRIAASQKANL
                     LDSLNQLVPAQRYDNDLLHDTLSINDNTLLHLDHDDTAYRARKNGQNVAVIFPVVAPD
                     GYSGKIKLLVAIEENGQLAGVRVLTHKETAGLGDAIEKQKSNWIDGFNFRSLANTTAQ
                     QWHVKKDNGVFDQFTGATITPRAVVKAVHNSLLYYQQHKNQLFAQPNDLIDDESEQLL
                     SQYP"
     gene            complement(25860..26873)
                     /locus_tag="PLY05_00145"
     CDS             complement(25860..26873)
                     /locus_tag="PLY05_00145"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNC01742.1"
                     /translation="MSFITVTSPHQHGQQRTAWVMRQVCYATLPALAALTFSFGIGSI
                     INVLWCVAIALILEGICLKLRKKNLEFYLSDGSAVVTGLLLGLSISPLSPWWLSVIGL
                     IFAIPIAKHLYGGLGQNPFNPAMIAYALLLVSFPVEMTRWPTGMNSIDSTLHIFLDQY
                     GAVDLTTGATPLDNFKTNHHSPTLTWLANGNIDLPESMMVNVMFALGGLYLLAKKIIT
                     WHIPVSILACLTVLSTLFFMIDSQHYASPMLHLLTGATMMGAFFIATDPVTAATSNGA
                     RLIYGVGIGMLIYIIRTWGGYPDAVAFAVLLMNLAAPMIDHLYQPRTFGHQVSIVKWR
                     NKS"
     gene            complement(27010..27594)
                     /gene="pth"
                     /locus_tag="PLY05_00150"
     CDS             complement(27010..27594)
                     /gene="pth"
                     /locus_tag="PLY05_00150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNC01743.1"
                     /translation="MAGLQLIVGLGNPGLEYAQTRHNAGVWYVAALAEQYNIRMTPES
                     KYFGLVGRGQINGQEVRLLIPTTFMNRSGQAVSALANFFKISPDAMLVAHDELDLPVG
                     SIRLKTGGGHGGHNGLRDIISGLGNQNNFHRLRIGIAHPGDAKLVSNYVLGKAPAAEQ
                     EKIESAIIAAVKVTDLMINGNLNKAMNQLNGFKA"
     gene            complement(27608..28186)
                     /locus_tag="PLY05_00155"
     CDS             complement(27608..28186)
                     /locus_tag="PLY05_00155"
                     /inference="COORDINATES: protein motif:HMM:NF004130.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HNC01744.1"
                     /translation="MSANFNLTAVSRKDEGKGASRRLRHAQQVPAVIYGAGQAPQSIA
                     IPFKDLVKALESEAFYSHILTLSIDGQEQQAVIKALQRHPAKNIPMHADFLRIDATHK
                     ITMKVPLHYLNQESCVGVKAEGGAISIIANDVEIACLPKDLPEFLTVDFAAVHIGTTL
                     HLSDIALPAGVEIPLLAQGHDYDQPIANVHKA"
     gene            complement(28303..29253)
                     /locus_tag="PLY05_00160"
     CDS             complement(28303..29253)
                     /locus_tag="PLY05_00160"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNC01745.1"
                     /translation="MPNLVVFTGNANPELAQKVVDRLHVPLGRAQVTRFSDGEISVEI
                     NENVRGKDVFILQSTCAPTNDNLMELIVMADALRRASAGRITAVVPYFGYARQDRRPR
                     SARVPITAKVVADMITSVGIDRVLTVDLHADQIQGFFDIPVDNIYGSPVLLADLQKQS
                     FDNLMIVSPDVGGVVRARAVAKQLGEVDLAIIDKRRARANESQVMHIIGDVSGRDCII
                     VDDMVDTAGTLCKAAAALKEHGARRVVAYCTHPVLSGAAIKNIVNSAMDELVVTDTIP
                     LSAEAVASGRIRQLSLAPLLAETVRRINNEESISAMFDLF"
     gene            complement(29335..29409)
                     /locus_tag="PLY05_00165"
     tRNA            complement(29335..29409)
                     /locus_tag="PLY05_00165"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29375..29377),aa:Gln,seq:ttg)
     gene            complement(29625..29699)
                     /locus_tag="PLY05_00170"
     tRNA            complement(29625..29699)
                     /locus_tag="PLY05_00170"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29665..29667),aa:Gln,seq:ttg)
     gene            complement(29698..30543)
                     /gene="ispE"
                     /locus_tag="PLY05_00175"
     CDS             complement(29698..30543)
                     /gene="ispE"
                     /locus_tag="PLY05_00175"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004739813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNC01746.1"
                     /translation="MLSTLTLPSPAKLNLFLHITGQRADGYHNLQTIFQFVDYADTLT
                     FTLREDGMITLSPALADIASQDNLIVKAARLLQQQSQSQLGVDIHLNKIIPMGAGLGG
                     GSSNAATTLLALNILWNLHWPNEKLAQLGLSLGADVPIFVYGRAAWAEGVGEALTPLE
                     LPEPVYVVLKPDCKIGTREIFTHPALTRNTPPITVSPHAAQHGQNDCEAIVSELYPPV
                     QNALLWLRQYGNAKLTGTGCCIFVDCSTVEQANKILAKSTVDGFVTRGYNLSPAHKAL
                     QKYLS"
     gene            complement(30543..31226)
                     /gene="lolB"
                     /locus_tag="PLY05_00180"
     CDS             complement(30543..31226)
                     /gene="lolB"
                     /locus_tag="PLY05_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HNC01747.1"
                     /translation="MRHFFTATTLILLQGCTSQSTILTFKEPYKIPTSTPVVAAHPLT
                     NLTVSEDVDTIQLPELPAQSMWTASGKFSVKMIDQTGKKKGGSAYFLWQQDTQDYHII
                     LTGPLGQGRTSLVGNANGVIMQSAQTGEMSADDPETLFEQAFGWTAPVSYMKYWLEGR
                     AATPTAVLNYAPSGQLESVHEGNWQADFKNYRYVNTQLLPQKIVVTGPNVNMTVLISD
                     WQPQVVATQ"
     gene            complement(31230..32906)
                     /locus_tag="PLY05_00185"
     CDS             complement(31230..32906)
                     /locus_tag="PLY05_00185"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC01748.1"
                     /translation="MNKTLVKIYRTTALITLTLANFSYANTPQNNHNLSYDSLYGLLL
                     GEMALQRNQTNLALEQYSQQAQQTKDKAIIERALQIANFLQDNEQGLNLAKLWIETTP
                     KDQKAYYQLAYHALKKQKYNQAIEAIDKLLLLEPESDLEALFLSAYPSLPEQRKQLLD
                     AIQNLSKNYPDNPHLLFAHGLLVGETGDYKQALIFIEKAQKLNPRSIPTVLLQARLLT
                     LNKQSDKAIKLLEQSLQKTPNSPQLNMHYIRALINDKQYNAAETKLAQQLLRQPQNTE
                     MLLMHALLSFDNQHDEQAIKSFNKLIVLNSNVDEAFYYLAMIAKRQNNLSQAESYFSQ
                     INDGDRFLSAQVELASLRIKDQRDVQMRQQFNESRQQHPEIANTLYAVEAEILRDHQR
                     LDEAYAVLADAINKFPQDNLLLFSRALLAEKRNDLIRFELDMLELFKRDSKNPSYLNA
                     YGYTLADRTNRLEEADRYLREAIKLKPNDPAIMDSVGWLLYKQGHLFEALTYIKKAYA
                     ASKDEEIGLHLAEVLWVCGFKAEAAKIWQELLMQKPNSEAVLKHRAQWEK"
     gene            33047..34303
                     /gene="hemA"
                     /locus_tag="PLY05_00190"
     CDS             33047..34303
                     /gene="hemA"
                     /locus_tag="PLY05_00190"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNC01749.1"
                     /translation="MGFIALGINHKTASVHLREKVAFLPEFLPHALQVACARAEINEL
                     AIVSTCNRTEFYGIANDSQKLLQWLSQERQISLTELQTSIYLHQDQQALRHLMRVASG
                     LDSMVLGEPQIFGQVKVAYQHAQQAGTVGSQLDRIFQQAFAAVKRVRTETAIGANPVS
                     IGFAAVQLAKQIFTDLTKANALLVGAGEMISLVAKHLKEQGIKHITIANRSLERAENL
                     ATELGGAKVVLLADLAEVLPQADIVISCTGSQLPVIGKGMVERALKKRRYQPIFMVDI
                     AVPRDIEPEVDDLEDVYLYTVDDLESTIEENRRARQQAAQQAEQLIEQCAIEFGHQQR
                     AQAASGLVMAYRQQVEELRLAEIAKAQQSLAQGVNPHDVLERLSRNLTNKLMHTPSVQ
                     LKQAAANNAHDKLMWAAELLGVQQNE"
     gene            34396..35481
                     /gene="prfA"
                     /locus_tag="PLY05_00195"
     CDS             34396..35481
                     /gene="prfA"
                     /locus_tag="PLY05_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003669771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNC01750.1"
                     /translation="MKASIRSRLEQLSERYEEVTALLSDSSTISDNEKFRKLSREHSE
                     LGEITQVWQAFCQAEQDLATAQEMKADPEFKEIAEEEIDAALKRIDQLTEQLNVLMIP
                     KDPNDTCPVYLEVRAGTGGDEAAIFAGDLFRMYSRFAERQGWQVEVISCNEGEHGGYK
                     EVISRVSGEGVYGTLKFESGAHRVQRVPETESQGRVHTSACTVAVMPEPEEQAAIEIN
                     PADLRVDTYRSSGAGGQHVNKTDSAIRITHIPTGIVVECQEERSQHKNRAKAMSWLAA
                     KLESRQREAQQQATATMRRDLVGSGDRSERIRTYNFPQGRLTDHRINLTLYRLDAVME
                     GDLTEVVEALAREYQADQLAALANDES"
     gene            35488..>35839
                     /locus_tag="PLY05_00200"
     CDS             35488..>35839
                     /locus_tag="PLY05_00200"
                     /inference="COORDINATES: protein motif:HMM:NF037644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01751.1"
                     /translation="MTLSIQQFQHLARQRLSNEDAALEIELMLCQVLNKNRIFLRTWP
                     DTLLTETQLEQLNENLQQREQGVPLAYLLGERAFWTLNLTVSREVLIPRPDTECVVEK
                     VLALGQGKQWRVVDL"
BASE COUNT        10644 a         6419 c         7510 g        10966 t
ORIGIN      
        1 caagatggtt tgagtgatgc cgagattgac gccttaattc aagcacgagc tgatgccaaa
       61 aaagccaaag actttgctca gtcagataaa atccgtgccg atttattggc aaaaggtatt
      121 attttggagg actctcgcca aggcacaaca tggcgtagag gctgaaaaat aagcggttaa
      181 cgtcaaaatg taaaaaaagc cattttgatg cttgacctat aatttaaatg gcctataatg
      241 cgcgcctact tgatgataac gtcgttaagt aaataagcgg gaatagctca gttggtagag
      301 cataaccttg ccaaggttag ggtcgcgagt tcgagtctcg tttcccgctc caaattttaa
      361 aaagccgatt ttacaatcgg ctttttttat ggctaaaatt tgtttttagg gtaattctat
      421 gctgtcagcc agttctgcac ccattggtgt gtttgattcg gggtatggtg gtttgacggt
      481 tctcaaagaa ttattacagc atttgccaga atacgactac ttatatttgg gcgataatgc
      541 ccgtacccct tatggcacac gctcctatca aaccgtgtat gaatttaccc tcgaatgtgt
      601 tcgttatttg tttgatgcag gttgcccatt agttattttg gcgtgtaata cggcatccgc
      661 caaagcatta cgcaccattc aacagcatga tttaccatta ttagatgcat ccaaacgtgt
      721 tttaggtgta atacgaccta gtgttgagca agttggctta ttaactaaaa caggacatgt
      781 aggaatctta ggaacaacag gtacggttaa ttcggaatcc tatcgtttag aaatcaataa
      841 gttattccca gaaattcaag tgtttcagca agcttgtcca atgtgggtgc ctttggttga
      901 aaatggtgag tatcaagatg agggtgctga tttttttatt aaacgtgatt taggcttatt
      961 gtttgcacaa agtgaaaaaa ttgatacaat tattttgggc tgtactcatt atcctttatt
     1021 aatcaataaa atacgtcaat acttgccaag tcatatacag gtattatctc aaggagaggt
     1081 ggttgctcat agtctaaagg attatttgca gcgacatact gagatggcta aacgttgttc
     1141 tcaatttact caacgtcagt ttttaaccac agataatccc gaaacattta gtcaattagc
     1201 cagtttattt ttaggtcaaa gtatagatgc tcaaaaaatc tcattaagct aagatggttt
     1261 agcagcatct atatctagcc aattttgatg cttagtggta ttgtcttaaa gttacaagct
     1321 tattatataa agcaaaagta tggtaactag tttgcaaaaa aaatgttcaa aatggattgc
     1381 tatagtttgc ttgctaaagt tatcgtaagc ttctgatgtg ttgattagat ataatttaaa
     1441 aaatctgaat ttttgtttgt caatgcttta aaagtatttg ccaaactcag taatgagtaa
     1501 aaatataaaa cctatggatt gtgctgcaat cagtgttttt taagcgatag cctaggtttt
     1561 aaaaaattag tattcaaatt taatagctat atattgctac atttttagga agtttaattg
     1621 tgaccgataa acgctaccaa gtttttatca gtgcgaccta tatggacttg cgtgaagagc
     1681 gaggtatgtt attgcagacc ttaccatcgc taggatgttt accaagtacc tttgaagctc
     1741 acacgcaaag tctaagtacc atggttaata ttcgccgtcg aattgatgaa tgtgattatt
     1801 atttgcttat tgttggcagt cgttatggtt cactaatgcc ctctggtgtc agttatactc
     1861 atatggagta tgtttacgcc gcgaccaaac aaaagccaat gttggtattg atgcatgaat
     1921 ctcctgaaac tcgccctaca gagtttcagg agcaaagcat tgaaggaaaa ttaaaattta
     1981 atgattttcg taaagtgttg cagcgcgagc gtgacaatat cgtttattgg cgtgatgtta
     2041 aagatttaag tcatgcctta cataatgcta ttccagcttt aattcgtaaa tacccaactg
     2101 ttggttggtc aagagtcatt aaaggagcga gtactggtgc cacaagtaca gtttctaatt
     2161 ctgcactaga aaaagagtgt gaagccctaa aaagacgtgt caaagagtta gaggctgaaa
     2221 aagaaaaatg gttaagtgct ggtgtcaata gcccagaaac cttagccaaa ggtgaagact
     2281 tatttgacat tcaatacaag tgtaaagcct atgctggcgg caactgtcaa gatgtatttg
     2341 gcaaaactcg cctatcatgg aacgagttat tggttagttt tggctccaac ttattgcagg
     2401 ctcaaacaga agagtttatg gctgctaagt taaatgaacg cttacaaaag aatgccttag
     2461 ctgaaataca aagcgaatat cctaaaactc atgctgttgt tgatgtacaa attacgcctg
     2521 caacatttaa tgccataaaa atgcaattta gagtcttagg atatattcaa cgtgtttcac
     2581 gagagaacga tctcaaacaa tggtggcaga tgaccgcgat tggtgagcgt atgttggcta
     2641 gtattatgag tgttagacgt acagcgagcc gattagctta aatgaccttt ggtttttatc
     2701 gatgtgtcga tcagtctaag ttaacttatt gttgaggtac ggtatgcgtc agtggaatag
     2761 ttggggtgat agtgaaacta aaatggcaat taatgccaaa gcagcccaat ttttacaaga
     2821 gcttattggc cagtcaactg ccttagctga tgcgacttta gcagaggtga tggcaaaagt
     2881 tcctgtcaca cggttaccta aacatccatt aatttctata gacgctgaag atcgagttcg
     2941 ccatgcgcgt ggtcaaagtt tgcctgattg gttggcatgg cgttcaggtg agattcccgt
     3001 ttttccagac ggcgttgcct ttcccacaaa tagccaacaa gttgctgagt tattagctct
     3061 tgccaaaact cataattggg tagtgattcc ttatggtggc ggtacttctg ttgttggtca
     3121 tattaaccct attgctgacg aacgtcccat tttaaccttg tcattagcgc gattaaatca
     3181 attgatcgcc ttagatgaag cttctcaatt agcaacaatt ggggcaggtg caaacggnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngaa gctgttgttc gtgttagccg
     3361 tacgccaaac aaagaagtgt tttgtgttta ttttgtgcct gattgggcaa aagctaaagc
     3421 agcagtacgt gaattagtcc aacaaaaaat tcccttgtct atggtgcgtt tgagtaatgg
     3481 ggaagaaact cgtactcaac tgattttagc aggccatgcc ttagccattg cgggtttaga
     3541 aaaagtgcta aactggcgtg gtgttggcgc aggtaaatgt atgttaacta tcggttttac
     3601 gggtagtgag caacgaatta agtttgcacg taacgaagtc actcgaattc ttaaagaatt
     3661 tgattgtgtg gcaacaggtc aggtgttggg caaacgctgg gcgcaaaatc gttttcgtgc
     3721 gccgtatttg cgtgaggctt tatggtattt agggtatgcg gtggataccc tagaaacagc
     3781 cgttgattgg cataaagtga cgccatatat gactgatgtt gaacaagctt tacgttctgc
     3841 attacaagat gagaatgagc atgtattggc ttttagtcat ttatcgcatg tttatggtca
     3901 aggatcgagt atttatacca cttatgttta tagagtggcg ggcaattatc aacaaaccta
     3961 tgctcgttgg caaaaattaa aggcagcagc atcagcacaa atcattaagc acggtggcac
     4021 tattagtcat caacatggtg ttggtcaaga tcatgcaccc tatttacctg ccgaaaaagg
     4081 tgtattgggt atgtctgtat taaaaacatt ggcaagccat tttgatgtag ataagcgtct
     4141 aaatccgcat aaattattag aggattaagc caataaagtg gctgtttatt tttattaaat
     4201 aaacgctttg tcggctattt atctgatttg tataatcttt gttgatgaaa aattgatgtt
     4261 ctaagtcctt aaattgtcag ttaagaacaa tcattttaat aaaagaccga gtatcctttg
     4321 tttattcggt tcttttagta tctaaggtaa atacatgtta aatcgtgatg tcttgtggca
     4381 agagttgcct caaacagaat gggatattat tgttgttggt ggtggtatca caggtgcagg
     4441 tgttgctcgt gaagctgctc gtgcaggtgc aaaggtttta ttaattgacc aaaaagatta
     4501 tgcctttggc acatccagcc gttcttcaaa aatggtgcat ggcggattgc gttatattgc
     4561 gatgggtgat atcaaattaa ctcgtcactc cttggttgaa cgtgagcgtt tgttgcgtga
     4621 agcaccgggc ttggttgacc gtatgagtta cttttttgcc cattaccaag gtggtggaat
     4681 caaaagtaac cgttgggcat ttgcgatttt attaatggct tatgatattt tggcaggtat
     4741 taaagaccgt agttatttta atgctaaaaa gtttttagcc cgtgtgccaa attaccgcag
     4801 tgataaatta ctaggtgctt cacgttatac ggatgcagtg actgatgata ctcgtttagt
     4861 gattcgagtt ttggatgaag cttgtaagga tggagcagtt accgttaact atgttaaagc
     4921 cgaaaaaacc attattacca atgatcaagt gacggctctt gtggtgcgcg atgaactgag
     4981 ccaagaaaca gcgacattga aggcaaaagt agtggttaat gcaacgggcg catgggtcaa
     5041 tcgtttgcgt caggaagttg ctacagagac tgtcagtgtg cgtccacaac gaggtagtca
     5101 tttagttgtt tcggccgaac gcttaccctt aaacgatgct ttaattttat ttcatcctga
     5161 tgataatcgt gccatgttta tttatacatg ggagggtcgt acggttattg gtacgaccga
     5221 tttagatcat aaagataatc ttgatgatga agcggtgatt acggctgagg aggttaaata
     5281 cttattacgt gcagctaatc atgaatttcc tcaagctaaa ttgacaccaa atgatgtaat
     5341 tgccacattc tctggcgtgc gtcctattgt gagtagtggt aaaggtctag acccatccaa
     5401 agagcgtcgt gatcatgccg tatgggctga taaaggtgtg attagtgttt caggtggtaa
     5461 attaaccact ttccgtcaaa ttgctcaaga tgtattaaca gcggctgagt cttggttgcc
     5521 aacaattaaa caacgagatc aaaaagcgtc tgtgtttagc aaacctgaag gacatattag
     5581 tagtccatta tcgccagacc aatatcgccg ttttgtgggt aaatatggct atttagcaca
     5641 acgttttttg gctgaaatgc cagctcaaga gttaaagtta attccagaaa ctcaaaccat
     5701 gtgggcagag gttcgttggg cattgcgtta tgaacaagtc gttcatttag atgatttaat
     5761 gttacgacgt acgcgtttag gtttattgtt ggtcaaaggt ggtgctgccc attttgacac
     5821 catcaaacaa atggccttga gtgaaggttg gacagaagaa cgctgggtaa gtgagcaagc
     5881 tcgttatagt caaatttggc aagcttatta cagtttgcct aaattatagg gtgggtcatc
     5941 gacccatcaa tttgttgatg gaatgattag catgaataaa gcacgttttt tgagtataga
     6001 ttgtggtaca caaagtttac gtgcttttat ttttgatgcc aaaggggagt tgttagcgcg
     6061 taatcaaatt cattttgagc caccttatca atcacccaaa cagggttttg ctgaacaaga
     6121 tgctaattat tattggcatc aattagtgac tgcttgtcaa tcattatggc agcagggtat
     6181 tgatcctaat acagtgacgg ctgcaagtgt taccactcag cgtggtacaa cggtaattgt
     6241 tgatgaacga ggtcaacctt tatatcctgc ggttttatgg ctagatgaac gacaaagcga
     6301 aaaattgcca gcattgcctg tttattggca aaaactccat caagtcagtg gccatcaaca
     6361 aactatttta aaattacgta aacgtgcacc tgccaattgg tttattagtc atcgtccaga
     6421 attatgggca aaaacctata aatttttatt gttatcgggg tatttaaatt acaaactaac
     6481 aggtgaatat agtgattcta ttccttcaca agttggttat atcccttttg attataaaaa
     6541 gcatcaatgg catagtcgtt gggattggcg ttggcaggcg ttgggtttaa cagctcaaaa
     6601 attgcctaaa ttgattccag caggcaaatt aattgggcag ctaaccgttg ctgccgccca
     6661 tgaattaggt ttgccagcag gcttggcgat tgttgccgca ggcgcagata aggcttgtga
     6721 agtcttaggt gcaggatgtg tgaatgctac ccaagcggca ttaagttttg gtacaacagc
     6781 cacttataat accgtgactt cgcgttattt atcgttaaat ccgcctatgc caccatatcc
     6841 tgctgtattg cctaagcagt attgtttgga gacccaaatt cctcaaggat ttgctttagt
     6901 taaatatttc aaagaacaga ttgctgcgaa cgaaattgcc gaagcacaac aaggcagaga
     6961 ttggttggct gttcttgagg attggttaaa tgcaacacca gcaggtgcta acggcttgat
     7021 gtggcagcct agtttgagcg tgcaagcatc aggtgaaagt gtgtcaaaag gagccttgtt
     7081 aggcttaagt acgcaacata ctaaagctga ttggtatagg gctttagttg aaggcttatt
     7141 gtttgcctta aaacaaggcc aagaacacgt cgagcaacat accaaacaaa aaattgcaca
     7201 gctttttgct gctggtggtg gttctcagtc taatgccgtt atgcaaattg ctgctgatat
     7261 ttttaatatg cccatcagta aaccacatac cagtgaaact tctggtttgg gtgctgcgat
     7321 gtgtgcggca gtcgcaatgg ggatttatcc cgatctgaat caagcggtac gagccatgag
     7381 tcgcaccgaa aaagtttttt accccaatac aatcaatgca cgtttttatc agcaacgtta
     7441 tcaatatgtt tataagcctt gggttcaaag cttaatacaa ttgtatgcaa caaactgaat
     7501 tgcttgaaaa aacacgcgtt aaccgccatt attagctaaa tattaattga tttaggtttt
     7561 tgctatgaag tggcgtcagt ggttagtggc atctagtttt gctgtgttaa cagcgtgtac
     7621 tagtagtact catgaaggag caacaggcat tcagcgcaag cagtttttat tattgcctgc
     7681 agaacaagtg aatgcaatgg cactacaatc ttatcgtgga gagctgtcgc aagccaaaca
     7741 aaaaggcgcg ctcaataccg ataaagctaa tcttgaacgt attcgcaaaa ttgccgatcg
     7801 tctcattcct caagttgcaa cttttcgtcc agatgccctg aattgggcat gggaagtcaa
     7861 tttagaaagt cgtaatgaat taaatgctta ctgcgcaccc ggcggtaaga ttatgttttt
     7921 tacaggtatt attaataatt taaaactcac tgatgacgaa atagccgcaa ttatggggca
     7981 tgaaattgct catgctttgc gtgagcatgg tcgcgagcgt atttcgcaag cctatgctca
     8041 acaagcaggt atggctattt tggcgatggt gacagacatg agccaaaaac aagcagccat
     8101 tttgcaaaca gccactcatt taggtttaac attaccacat ggtcgaggcc aagagtcgga
     8161 agcagatgtc atgggcttag agctattagc tagagcagga tatgatccaa aagcatctgt
     8221 tactttatgg caaaaaatgg catctgctaa taaaaactca ccgcctgctt ttttaagtac
     8281 tcatccgtcg agtgcccaac gtattaatga attaaatagt aaaattacgg ttgttatgcc
     8341 attgtatgag gctgcaaaac gtaatcctcc agcatctaaa acaatagttc ctcgtactta
     8401 agagattatt cttatgtttc atagtttaaa tattttttcc tcggttgtag cttctacagt
     8461 acgtggttgg cgtggctcca aaggcagtaa aacaacacaa cagcctgttc aactgccaat
     8521 attgtttgat agtgaagata atgctgaatg tcgcttagta cgtgaggcat taacagagtt
     8581 aaatttggat gtgttgatct atccttgtcc acagggtgca gaccgttttg caggtgaatt
     8641 acaacgatac ggaggtgcta atgcaagtat cccttatttg gttgatccta atacaaatgt
     8701 caaattagaa ggggcagagg caataaatgc ttatttattt cagcaatata aacaacagga
     8761 aatacctaaa aatttagatg cgacacagtt aaatttgttt aattcgcggt tggcttcttt
     8821 tgcgcgttta agaataacgg cattacaggc aaaacctgca aaatcagctg ctcaaccact
     8881 catcttatat agttttgaat ctagtccata ctcacggcct gttcgcgaaa aattatgtga
     8941 attgcagttg ccatatcgtt taattaattt agggaaacaa cagttagcag atgttgggcc
     9001 agcaactttt cggtttcatt taggggagta tcatcctata cctaatagca aacgagcaga
     9061 gttattaaca acaaaaggtc gtgtacaagt gccttttttg atcgatccta ataacaatat
     9121 agaaatgttt gagtctaaag atattttgga gtacttagac aaaatgtacg ccttataaaa
     9181 acagactact aaataaaaaa accaccaaat ttggtggttt tttatttgat tttatatatc
     9241 aagtaagact tttatatcct acccataatg cgattaataa aatcagtccc caacctaacc
     9301 attcgacaat gcctctaacg cggtgttcca ttttttcgcc gccccaagcc atcaatccag
     9361 ctactaagaa aaagcgtgct cctcgcccta cacacgaggc caacacaaaa ggtaacagag
     9421 ccatgctcgc gaaaccagca gcaatagtaa aaactttata aggaatagga gtgaagcctg
     9481 caataataac agcgagtaca ccccatttac taaaccattc gtcaatggtt gcaaaatgtg
     9541 aagaccaacc aagttgctca aataacggca aaaattgatg gtaaaacatt gcgccaatag
     9601 cataaccaaa tactccacct aatacagaag caatcgttgt aattagggca aatcgtaatg
     9661 ctttcttagg ttttgtcatg ctcattggtg ctaacatgac atcgggcgga attggaaaaa
     9721 aagacgattc tgtaaagcta agtgctgcca aatagcgttc tgcatgagga tgacgagacc
     9781 aacgcaaagc catgtcatac attttggtga ataaagccat gatttaccta aacaattaga
     9841 aaaaataggt gtatgagtaa taatgtctta ttctacctta ggtttaacta taaaagattg
     9901 ttaaaaacaa aattagaaga tggatattgt gagtatcaaa agaagcgagt tgttgataaa
     9961 tatagctgga atatgcttgg ttaagattgt gcttttgata aaatagactt acgcatttta
    10021 cggaaattag cgcgttttgt gagctaataa atatcgcttg aaacgataat gccgcgtaag
    10081 tcctataaaa aacacaacct taaaactttc aaacaccaca ctcactgtcg gatggactga
    10141 ttttgcagcg tacttttttc attaaattca tcatgcgttc atcgtaatag ccttctttgg
    10201 ttagatggtt acgtgcttcg cgcatcatgg tgatataacg agttttttcg gcgtcagata
    10261 aatccaacca atattgttgt ggaatagctt tttctgtttt ctcgatatag gcaatcgttt
    10321 tgtccatttc acctaaagca tattgacgaa tttgctctaa acgtaaatct aaatctggaa
    10381 tttgtttagc gagattagtt cgattaatta aaactgtacc cgtcatttgc ataattggta
    10441 aacgataaat accaccataa taacttaaac ctttagacaa ctctaagggt ttaaaagcaa
    10501 tcgcaggagc agtaataata tctacttggc cattattaaa taaatttgca aaattataaa
    10561 aatccgtatt aattgcttga gcacctaata aatggactaa tttactgtga gctttatcgg
    10621 tctcaatcac ggctactttt ttaccagtcg ctttttctac cgagtcaata tgtcgattat
    10681 tgaccatgac atagcttgcc cctaaaggca tcacagtaac aacttgataa tcaccttgta
    10741 tggtgagcgg tgcaatttta gggtgattaa aggtgctgac taattcttta atatgtcgat
    10801 agcttggtgc agaaccaata gagtcaatac ttccaacaaa attattaaat tgtttagcac
    10861 gtaatgtgct aacagcaata ccatcacatt gattggcttt aaaatcttcg gcaatcactc
    10921 gttcatcggt ataggctcta attttagcgt tgacattcca tttttgagca aacaaagaca
    10981 agtcttttgc gtagcggtat aaatcgccgc cattacctgc cacatcataa acgcaaaaag
    11041 tcatatttag tggtttactt agcggattaa gtggagcagc aaacacattt gctgtatata
    11101 aggagctaat taacgcaagt cctgttacgt atttagtcgt tgataaacgt accataattc
    11161 gtacctcact tgaaaaacta atgtgtactc attagtacag ataatttagc attgttttgt
    11221 gacaaattga ttttatgttg tcctactgtt agacagtaaa tggcataatt tatcaatatt
    11281 taataatttt atatgagtta attaacaaaa atttattaaa ttcaattggt tattgtattt
    11341 taaataaata tacccatttt atgggtgact gatattttta aaagggagac aaataatgtc
    11401 ttatgattat gatgtaatcg ttgttggagc tggcccagca ggtgaagggg cggcaatgaa
    11461 actcactaaa gaaggcaaaa aagttgctgt gatagatgca cgtccacaag taggtggtaa
    11521 ttgtactcat gtcggcacga ttccatctaa agcgttgcgt caaacggttt ttaatataat
    11581 gagatatcaa cgtgactcga tgtttcaacg tgtaggagag ttgcgtaatg tgccattatc
    11641 acaagtattg gcacgtgcac ataaaattat tgaacaacaa gtagaagtac atacccgttt
    11701 ttatgatcga aatcgtgttg atatgtattt tggtcaagcg caatttgtcg atgaacatac
    11761 cttaaaagtg acgacaattg aaggtggtca tgagattttg acctttgaaa atgcagtatt
    11821 agccacagga agtcgccctt atcatcctac agatattgat ttttcgcatc ctcgtatttt
    11881 tgacagtgat aaaattttga cattaaatta ccccgttcat aaattgatta tttatggtgc
    11941 aggcgttatt ggttgtgaat acgcgagtat atttgttggt ttaggttaca aggttgatct
    12001 gattaatact cagcagcagt tattatcgta tttagatgat gaaattgccg atgcgctgtc
    12061 ttattatttg cgtgaattgg gggtgttaat tcgtcatcaa gagcaatatg attatgttga
    12121 aacttttgat gattatgtgg ttttacattt aaaatcaggc aaaaagatta aagcagatgc
    12181 tattttatgg tgtaacgggc gcacaggaaa tactgacaat ttagctttag atcaaattgg
    12241 cgtaaatgct aatagtcgtg gtcaaatagc ggtcgataaa caatatcgaa cctctatttc
    12301 taatatattt gcagcaggcg atgtgattgg ttggccatcg ttagcttcgg cggcttacga
    12361 tcaaggacgt tgtgctgctg cgcatatttt gggcttaaaa gacacgcctc atgttgaaga
    12421 cgtaccaacg ggtatttata ccattccaga aatttcatca ttaggcaaaa ccgaaaagca
    12481 actgactgaa gaacaagtgc cttatgaggt tggtcaggct ttttttagac atttagcgcg
    12541 cgcacaaatt acgggtgaaa cagtagggat gttaaaaata ctttttcatc gcgagacatt
    12601 agagattttg gggattcatt gttttggtaa caatgcagca gaaattgttc atattggaca
    12661 agcaattatg caacaaaaga atggtgggaa taccttgcaa tattttgcca atacaacctt
    12721 taattatccg acaatggctg aagcgtatcg tgttgcggca ttaaatggat taaataggtt
    12781 gttttaggaa gttgttcaat aaaatcccct ctaattagag gggattgatt gagtgttatg
    12841 tctcgtcttt agttgaacac tcagcaagtg taggttcaaa cttacagcga attttcttta
    12901 ataacaccat cattttaggg tcataaaagc cttctttggt catattaata cgtgcttcac
    12961 gcattaacgt ggtgtatttt tctttgtcag caggtgttaa atcgagccaa tattttgcag
    13021 gaatatctga ctcactctta ttaaccaatt tttctaccat atccattttg cttgcagcaa
    13081 actgacgcat tttaatgata cgttggtcaa aatcaggaat atctttgtat ttttcacgat
    13141 ttattaaaat agaagcggtg acttgtacta atggaaagcg gaaaattgca ccgttttcac
    13201 ccaatccacg atataactct aaaggacgga aggcaaccgc aggcgcacca ataatatcta
    13261 cttgaccatt gttaaatttg cctgcaaagt tagtaatatc tgaggcaaca ggttgagcac
    13321 ctagtttttg cacaattttt gcttgagctt tatcgaagtc taatacggca acttttttac
    13381 cagctgcttt ttcgacagag ttaatttttt tgtcattcac aaaaacataa gcaccaccta
    13441 aaggaattaa ggcagcaact tgataagggc cactaattgt taaactattt aatttagggt
    13501 tagctaaggt gttaactaat tcacgtacgt gtttgaaggt gggtaaagag ccaggtgaat
    13561 caacactgcc aataaagaag ttaaattgtt tagcacgcat agtcgaaata gctacacctt
    13621 cacattgacc tgctttaaag tcttcagcag cgacacgttc atcagtataa acttttaaat
    13681 caacatctaa attccacttt ttagcttcta agaccaaatc tttggcatag gtgtaggcat
    13741 cacctttagt gcctgcaata tcaaaaatac aaaatttcat tttgacagtt tgaggaaatt
    13801 cagcattagc aggtgctgcg attgcccaag agctaagcat tgtactgaat aaggtcgatg
    13861 ccaaaacaag actttttttc attgtatggg tctctgatta tgttataaaa aacgataaaa
    13921 agcagtatca tgaagcattt agccagttta gtttaaaatt ataaaacgtt tagaattgaa
    13981 caattagtgt caaatgtcat gttaataaaa ctattcgtcg gtttgactac attcagcgcg
    14041 ttctggatta gttttgcagc gtacccgttt taaaatacta atcattttag gatcgtaata
    14101 accttgttta gtcatttcaa tacgcgcagt ttttagcatg ttccagtatt tttgttcatt
    14161 atctggatca attgttaacc aatatttact tggaattgca gcttcttctc gatcaatcca
    14221 ttggtaagtt tgtgttaaaa tttgatcaat aaatttatcg gctattgttc tgaatgcaac
    14281 aagtttttca tctaaatctg gaagtttttg tttgattttg gcacgattaa tgacaaaact
    14341 agcggtcatt tgtgttaatg gtaaacgata aataccacct gtttcgccta aacctcgatt
    14401 taattcaaaa ggtctataaa ctaatgcggg agcaacaata atatcaactt ggccattatt
    14461 aaatttgctt gcaaagttag ataaattaga gggaacggga tgtgcgccaa ttttattaac
    14521 tatttcagtt tgcgatttat cccaatctaa tacggcaatt tttttacctg ctgctttttc
    14581 gacagaatta atcatacgat cacgaacatg aatgtatgtg ccgcccactg gtaatacgcc
    14641 taaaatttga tatggctcag taatagttaa ttgcgagatt ttaggatcta gtaaaatacg
    14701 caataataaa cgtaattgct cataagtagt gatcccaccc acagcttcaa tactgcccat
    14761 aaataaatta aattgccgtg cgcgcatggt tgaaatccct gctgcatcac attggccagc
    14821 cttaaaattt tcggctgcaa cacgttcatc agtaaacact ttaatattgg caatgacatt
    14881 ccaacgtttg gcaattaaag ctaaatcttt tgctttacta taaaaatcac catttttgcc
    14941 ctgcatatca aaaaagcaca cagaaacatc cagtggtttt tctaaaggag gaggaagacc
    15001 atcttcattg gggtttaaat cattggctat agataaaata tcacctgcat ataggctagt
    15061 agataataag ccaagaacca atgttttgcc caacaagctt acatggcgat taaagaaagt
    15121 aacgattgaa gagaaggcca taaagaatat ccatcattaa taacttaaat ggctaatcaa
    15181 tacagcagag aaaagctatg ttttgagtga ataaatagct tatttaatat atcaatacaa
    15241 atagataatg gcttgcttta tgagtttagt cttttaagtt gttagattat tgttgtttta
    15301 ttcaacaggt tttgatacta ggacaatcag ttcataaaaa caagtctaat caaggcaaaa
    15361 atgaccgttt tcagagaaaa aacattgcca ataatataat ttgttacaaa tttaaaaatg
    15421 attttccagc gttgtttgaa tcaaacctgt tggccaaaaa ttgagaacta attcaaaaaa
    15481 agctatataa aaaacaagca attctatcaa gctaactttg tcggttttgg cagtttaaaa
    15541 caaaatcatg acaggaaaaa tcaataatgc agccagtttt ttaatttttt gatagatgaa
    15601 aaccatttat cgttaaaaaa atgcttttca tcggaaaata aagcgtctat tatttagtca
    15661 ctcaagcaat tgattgcttg gcgaaaacaa aaataatttt gcgtagctaa taacgcaatt
    15721 gacaataaaa atgggacacc acaatatgaa aactcaacaa aagatttttg ctagtctagt
    15781 tgttgcctta tcaacatcgt tagtaacaca gggtgcaatt gcttctactt atagtcaaac
    15841 taaatatcct attgtcttag cgcatggtat gtttggtttt aaaagtttat taggaggcgt
    15901 tgttgattat tggtatcaaa ttccttctga tttacaggcc aatggggcaa aagtctatgt
    15961 tacgcaagtg gctgctttag aaagttctga agttaggggt gaacagtttt taaagcaagt
    16021 tcaagatgta ttagcaatta caggtgctgc aaaagttaac ctgatgggtc atagtcatgg
    16081 aggacattct gtgcgctatg ttgcaggttt gatgcctagt aaagttgctt ctgtgacggc
    16141 cattggtagc ccagtcaaag gctctcctgt tgccgattta gtgacacaaa caggtgtatt
    16201 aagtcctatt gcttatggtg cattaaatgc gttagcaggg ataatcgaat tgtttaatgg
    16261 ggctggtagc tttaatcaaa gtgccaaaaa tagtattgcc tcattatcaa ctaagggttc
    16321 tgccgctttt actgtaaaat ttcctcaagc tgtgccaaca actgcatgtg gtcaaggtgc
    16381 tgcaacggtt aatggtgtta aatattattc atggagtggc acaaaacgtt taacaaatgt
    16441 tttagaccca acagatgctt tagtaggtgc aacaggttta tttatcagta cggctaatga
    16501 tggtttggtg agtcaatgta gtagtcattt aggtgttgta ttgcgcgacg attattcgat
    16561 gaatcattta gatgaagtta atttgatgtt tggtttgaga gatatttttt ctactgatcc
    16621 taaatctgtt tatcgtagtc atgcgaatcg cttaaaatta gcaggattat aattaataac
    16681 aaaaaacccc gcattcgcgg ggtttttcta annnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16801 nnnnnnnnnn ngtgttggca gtaccgccag ctttcaatgc gttgtcacca gagaaaatct
    16861 ctttgtgatc gtcaccgatg tcagaaccag ccattgcttg gtgcttaacg caagaaacac
    16921 cttcacggat ttctttacgt tgtacgccag caacataagc caacataccc atttcaccga
    16981 agtaaccttt agacaactcg tgcatatgta atgcagttgt gtggtaggtt ggtaaagtga
    17041 tgaggtggtg gaatacacca gcttcacgag cagcgtcagc ttggaacgta cggatttttt
    17101 catcagcgtc agcagacaat tctgtgttgt catacttagc gtccattaac ttagcgcgat
    17161 cgtatgcaga aacgtcttta ccttctttaa cccaacggtc ataagcttgt tgacggaagt
    17221 ttaaagtcca gttgaaagat gggctgttgt tataaactaa cttagcgtta ggaataactt
    17281 ctttcacgcg gttaaccatg tgagcgattt cgccaacgtt tggtgttggt gtttcgatcc
    17341 acaacatgtc tgcgccgttt tgtaagctgc tgatacagtc taaaaccacg cggtcgattt
    17401 gtgtaccttc gcggaattgg aacaagccag aagctaaacg tgttggacgg tgtaatttac
    17461 cgttgcgttt aatcaacact tcatcgtctt gagcttcaga gatatcaatt tctttagtat
    17521 ctaagtagct gatgtattga gaagcgatgt cgccagcttt cttaacaact gggatcttct
    17581 gagttaagtc agcaccttca gagtcagtac gtgcaacgat aacaccttcg tcaacgccta
    17641 attctaagaa tgcgaaacga acagcattga tcttagataa gaagtcttcg tgtggaacag
    17701 tgactttacc agcttggtga ccacattgct tagcgtcaga tacttggttt tcgatttgaa
    17761 tagcacaagc acccgcttga atgattttct tagctaataa gtaagttgct tcttcgttac
    17821 cgaaaccagc gtcgatgtca gcaatgatag gcacaacgtg gctttcaaag ttgtcgattt
    17881 tagcagtcac ttcagcagct ttagcagcat caccagcatc ttcagctttt tgtaaagcac
    17941 ggaataaatc gtttaattct tttgcatcag cttgacgtaa gaatgtgtag atttcttcga
    18001 ttaattttgg cacagcggtt ttttcgtgca tagattggtc tggtaatgga ccaaactcag
    18061 aacgtaatgc agcaaccatc caacctgata agtagatgta cttacgttca gttgtaccga
    18121 agtatttttt gttggcaatc attttttgtt gcgcaacaaa accatgccag caacctaaag
    18181 actgggtata tttgctgctg tcagcatcgt atgcagccat atctttacgc ataatagcag
    18241 cagtgtactt agcgatttct aaacctgttg ggaaacggtt ttgaataatc atacgcgctg
    18301 catcttctgg atgaattgcg ccccaagtat tgcctaattt tgctttaacg gcacgaacat
    18361 gctcaagagc agattgataa gtcgccataa atgccttcct atgttttagg ttgagggata
    18421 aacagataaa ataaatcatc tatttaagaa tgtgcgaagt gtaaagttta tggcataatt
    18481 tagctaattt atcagattaa tcttcggtat ttattttatg aatcttgagc gnnnnnnnnn
    18541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntaagcagtg cgcgtgtctt tagaattatg
    18661 gcctctgatt atgctgaggc gactttgatt cctgctttag tcaaagcgat gcgctccgaa
    18721 gccccgaatg tggttttaga ttttttaacg ccttctgatg tgtcttatcg agatatggag
    18781 cagggtaaag ttgatttagc gatcaatcgt tttaatgaga ttccacaaag ttttcaccaa
    18841 gttctcgttt ggcgtgatag ctttagctgc ttattgcatt gcgatagtcc cattgttgac
    18901 cattttaatt taaaaactta cctagaagcg caacatattt gggtcagtaa aacaggtatg
    18961 ggagtgggct ttggtgttaa ccctgataaa tctggtggtt taggctggat tgaccaagcc
    19021 ttagcacgtt taggtaaaaa acgtaaaatc agtattttta caaggcacta tcaaatgcct
    19081 gctttattgg cagcaaatgc agatttaatc gccactttac ccagtcgtgt ggctaaatta
    19141 caagccacta atccgcgttt agtgattaaa gagccgccat tttatgtgcc agagtttgaa
    19201 ttaaaaatgg catggtgtcc gttgctacat catcatcctg cccatcgttg gttacggcaa
    19261 ttaattttgt ttgtggcacg tcaattaatt gaagatgagc gtaaacaaat ggaaagacaa
    19321 atcgccttgc aacaaatgat gggacgttat gaagattagt ctttaatggt ataatcaaca
    19381 aaatttttat tgtgcgtggt gcgtcaattg ctcactgttt ctgatttatg tgcagtacga
    19441 gatgatcgag ttttatttgc taaactcgat tttcaattac aagctggcga aatcatgcaa
    19501 gtagttggtg ctaatggaac tggtaaaaca accttgctaa atggtctaat gggcttggct
    19561 ccatttgaat caggggctat ttattggcaa ggcgaaaata ttcaagataa tccttccgta
    19621 tttaagcaaa atgttgcttt tgttggccat ttattgggtt taaaaccctt attaacggct
    19681 gttgaaaatt tacagttttt agcgcaactg catcaaaaat tcatttcttt ggcagatata
    19741 gaacaagccc taaaaaaagt tgggttatat ggttatgaag atgtacccat tgcgcgttta
    19801 tctgctggcc aaaaacgccg tgttgcttta gcacgtttat ttttacaaca agcgaagtta
    19861 tgggttttag acgagccgtt tactgccatt gatttggctg gtgtgcaagc cttagagcaa
    19921 tggttagtag atcatgctca acaaggaggc atggtattgt tgactacgca tcatgttttt
    19981 gagccagact tcccacttaa acatttagat ttagcacaat atcgtgtaca ggagggctac
    20041 taataatgtg gccgtcttta tgggctgtga ttcagcgtga tctcaaattg gtctctcgac
    20101 aaggtggcga atggcttaat ccattaagtt tctttttaat ggtgattact ttatttcctt
    20161 tagctattag ttcaactcct acaaaattgg cagcaatggc aggaggagtg gtatggattg
    20221 ccgcgttatt ggcggtatta ttgtcgatgg atagtttatt tcgtcaagat tatgatgatg
    20281 gtagtttaga acaattatta gtatcgcctg caccattgcc cttagtggta ttagctaaag
    20341 tgattgccca ttggttacaa acaggagctt gtttaacggt attaagtccc attgctgctt
    20401 tattgttgca tttgcccaac gatgcggctt gggctttatt tattacctta ttaataggta
    20461 cgccaacgtt gagtctagtc agttcgatag gcgcggcatt aacagtctct ttaaaacgtg
    20521 gtggtgtatt aattccgtta ctcaccttgc ctttacatat tcctgtgtta atttttgcca
    20581 ccgctgctgt ccaagcggcg ttgaatggtt taccaattgg tggttattta gccttgttag
    20641 gggcgttttt ggttttagct ttaacattaa gtcccttagc cgttgcggcg gctttacgtt
    20701 tagccgtggg aggttagcgc atgttttttt taaaacgttg gtggcaatta tttgaacaaa
    20761 ccgttagccc aaaatatttt tatagtatca gtgagcgatt tattccgtgg ttaagtgtgt
    20821 tagcagggtt gttgattgcg gtgggcacag tatggggttt agcctttgcg cctacagatt
    20881 atctacaagg taatagttat cgcattattt ttattcatgt gccttcagca agtttagcca
    20941 tgagtgccta ttttttatta ggtatttgtg gcgtgattag tttagtttgg aaagtaaaaa
    21001 ccgccgagat ggtggcgcga gctgttgccc cagttggtgc tgttttttgt tttttagcac
    21061 taatgacagg ggcaatttgg ggcaaaccga catggggaac ttattgggta tgggatgccc
    21121 gtttaacttc tatgctaatt ttattgtttt tatatatggg ggtaattgca ctgtataacg
    21181 cctatgaaag tagttcaaca ggtgcaaaag cggctgccat tttatcggta gtaggcgtga
    21241 ttaacttacc cattattaaa tattcggtgg tgtggtggaa taccttgcat caaggtgcaa
    21301 catttaccgt tacccaaaaa cctaaaatgc cgccagaaat gtggatgcct ttattagtca
    21361 tgattttagg attttatagt ttatttggat tattaactat tttacgcacc cgaaatgtga
    21421 ttttagagcg tgagcgtcgt agtcaatggg tcaaagagtt agtcaaaaca gaggcaggta
    21481 gagggggtca acgtggcgtt taattcttta gctgaatttt gggcgatggg taatcatggc
    21541 atttatgtat ggtcgtgcta tgccttaacg tttagtgcga ttctctattt ggtatggcaa
    21601 gccaaagcac agcgtcagcg tttttttaaa gagcagattc aacagttaaa acgaggtcaa
    21661 cagcatgaat cctaaacgtc aacgtaaatt ggtattaatt atttcgttgg tactagggat
    21721 cggtgtggca attggcttag caatgtatgc catgcgtcaa aatatcaatt tgttttatac
    21781 gccaagtcag ttggcaaatg gtgaagcacc tgatggtcaa cgtattcagg caggtggttt
    21841 agtagtaaaa ggaacggttg ttcgagaccc tagttcttta gcagtcactt ttatgattag
    21901 tgatttaaaa aagcaagtgc ctgtaactta tacaggaatt ttgcctgatt tatttaaaga
    21961 aggtcagggc attgtggcta atggctattt taaaaatggt cgtgtagaag ctgaagaagt
    22021 tttagctaaa catgatgaaa actatatgcc gcctcaagtt aaacaggcga ttgaagaggc
    22081 gggacatcca acaggtaaat aaaacaggtg agacaaaatt atttatttta tttacgctgt
    22141 ttagccatac taggccaaag catcaccatt ggcctagtgc atttttttct gcaactcagt
    22201 ttaccgctat tgcccttgat cctcaccttg tcggttttag tggcagccaa tattcattta
    22261 gcgtattacc tcaaaacaca tcctaataaa gataccgatg actttatcgc gattcaatta
    22321 accatagata tgttggcctt agcaacctta ttgtatttta cgggtggcgc acaaaatcca
    22381 tttatctttt tgtttttatt gcccttagtg attggtgcag ccatgttgcc tgtgggttat
    22441 accatggctt tatttttatt gagtgtgttt ttatttacta ggctgatgtt cgattatgta
    22501 ccactcgaaa acatctcaca aaaacttccc ttagcaatta atggctatgt attggcaatg
    22561 tggatatgtt ttgtgttaag tgctgtatta atcgtatctt ttattgccta tttggcctat
    22621 caagtcaaac attcacaaca aaaaatggca aaaatacaag aaatccaaca tcgccaagaa
    22681 caaattttag cattaggaac tttggcggca gggacagcac acgagctagg tacaccgcta
    22741 gctacaatta cggtattggc gcatgagttg aaaagtgaag tagcagagtc tcttaaacct
    22801 gatatggagt tattgtgtca gcaagtcaat caatgtaaac aaattttgac acagatgaca
    22861 caacgtattg agcaagccaa aaatcaagaa tttgacttat tatctttaag taaattatta
    22921 gagcttgtct tagataaatt tcaattatta agaccaacaa ttcagattaa acatgccgat
    22981 tttggtgggt taacagatat tttagtggcg acagatgcga ctttagaaca agcgatatta
    23041 aatttattaa ataatgccgc agatgcctcg cctgattatg tggaattaac ggcttatatt
    23101 gatagctcac gtgtttatat taatgtgatt gataaaggtt taggtatgtc tgatgagata
    23161 ttgacctcat taggacaaaa tagaatttca accaaaggcc aacaaggaat gggtattggt
    23221 ttgttattag ccaatgcaac tttagaaaga ttgggtggag aaatgaactt tactcaccga
    23281 cagggtggcg gtgtggttgc acaaattgtt ttacccattt atcagcgagt gattcatgtt
    23341 ggataattta ccattgttac tgattgttga tgatgatcat atttttagac aagtgttagc
    23401 caaagccatg ctcaagcgtg gttacgaggt catccaagcc gaaagcgttg aacaagcaat
    23461 tgagttaatt gcgcttcaaa cacctgaata tgcagtgatt gacttaaaaa tgaatggtgc
    23521 gtcagggttg gaattagtga agtatttgca tgatttagat gagcaaacac gaatggtggt
    23581 attaacaggt tatgcaagta ttgcaacggc tgttgaagcc atcaaactcg gcgcaacaca
    23641 ttatttagca aaacctgcaa atgctgatga aattatggct gcttttttgc gtattgaagg
    23701 agattcagct ttgttagtga atgacaatcc tttgtcggtg gatcgattag aatgggaaca
    23761 tattcaacgt gttttacaag agcatgaagg caatatttcg gcaacagcga gagcattaaa
    23821 tatgcatcgt cgtactttgc aacgtaagtt agcaaagtat cctgttaagt tttagatgga
    23881 cataaatctg ctaataaaca cgtctcacat tgcggtttac gcgcagtaca tacataacga
    23941 ccatgcaaaa ttaaccaatg atgcgcatct aatttatatt gattaggcac gttggctaac
    24001 aattgttttt caacgccatc aactgttttt gagttggcta aacccaaacg attagccacc
    24061 cgaaaaatat gagtatcaac cgcaatggtt ggctcaccaa acgctgtatt taacactaca
    24121 tttgcggttt ttcgaccaac acccgctaaa gcttctaaat cttcgcgctg atgaggcact
    24181 tgtccttgat gcttgtctat taatatttgg caagtggtca taatgttagc cgctttgctg
    24241 ttatataaac ctatagtttt gatataactt tttagaccat ctaagcccaa ggccaaaatc
    24301 ttttgtggcg tattggcaat agggtacagc ttggcagttg ctttattcac actcacatca
    24361 gtggcttgag ccgataaaat aacggcaatc aataactcaa agggcgagtt gtagactaat
    24421 tcggtggtcg gtttggggtt ttgttgctgt aaacggcgaa aaatctcatt aattgtagag
    24481 gcattcatta gctaatatgt cctgtggttc tgactcgttt gctacctgcc acaacctctt
    24541 tggcttgttg gcttgcacgt tgttttaaac gttcatcaat cacatttttg cctgcaataa
    24601 taaaacctaa acaaataaag gcactaggtg gcaaaataaa aaacaaaaac tgcgtgtagt
    24661 cggcaatttt tatcgtccat gattgggcaa tattgcctaa taataaatgc atattggcga
    24721 ataatgtacc gttacctaat aattcacgca ttgcccccaa cagcactaac accacggtaa
    24781 aacccaaacc aaccatcaag ccatcaaatg cagcaaaacg ggtattgtgt ttagaggcaa
    24841 aggcttcagc acgtcccaac aaggcacaat tggtgacaat taacggaata aaaattccta
    24901 aagcttggta aagctgatag gtaaaggcgt gcattaataa ttcgatacac gacaccaaag
    24961 aggcaataat cattacaaaa ataggcagtc gtaaattggc aggcgtgtga tgtctaagca
    25021 aagaaacagc cgcattagaa ctcgccaaag ctaaggtcgt ggctagcccc aaacttaagg
    25081 cattaaccac tgaatttgag caagctaaaa ggggacataa acccaataat tggacggtgg
    25141 cggtgttgtt gcgccataag ccatcaacag caatttgacg gtaactggtt tcgctcatgg
    25201 atattgactc aacagttgtt cactttcatc atcaatcaaa tcattgggtt gtgcaaaaag
    25261 ttggttttta tgttgttgat agtacaacaa actattatgc accgctttaa ccactgcacg
    25321 aggcgtaatg gttgcgcctg taaattggtc aaatacacca ttatcttttt taacgtgcca
    25381 ttgttgagca gtggtattgg ctaatgagcg aaaattaaaa ccatcaatcc agttggattt
    25441 ttgcttttca atggcatcgc ctaagccagc agtttctttg tgtgttaata ctcgtacgcc
    25501 tgccaattga ccattttctt caatcgcaac caataattta attttgccac tataaccatc
    25561 aggtgcaacg acaggaaaaa tgacagcgac attttggcca tttttacgcg cccgataagc
    25621 cgtgtcatca tgatctaaat gcaataatgt gttgtcgttg atgctaagtg tatcgtgtaa
    25681 taaatcattg tcataacgtt gtgcaggcac taattgattt aaagagtcta ataaattggc
    25741 tttttggctg gcagcaatac ggtcactggt catggtattg acaatggcaa ttaaggcagt
    25801 ggtaatggct gcaaatacgg ccaatatcaa accacttttt agactttgtt cacgaatact
    25861 catgatttat tgcgccattt aacaatactg acttgatggc caaaagtacg tggttgatat
    25921 aaatggtcaa tcatcggggc ggccaagttc attaataaca ctgcaaaagc cacggcatca
    25981 ggatatccac cccacgtgcg aatgatataa attaacatac caattcccac accataaatt
    26041 aaacgagcac cattggaagt ggcagcagtg acagggtcgg tagcaataaa aaatgcgccc
    26101 atcatggttg cgcctgtcaa taaatgcagc ataggtgacg cataatgttg gctatcaatc
    26161 ataaagaaca acgttgataa cacggttaaa caggctaaga tgctcacagg aatatgccaa
    26221 gtaataattt ttttggctaa taaatacaag ccacccaaag caaacatcac attcaccatc
    26281 attgattcag gaagatcaat gttgccattg gctaaccatg tcagtgttgg gctgtggtga
    26341 ttggttttaa agttatctag tggtgttgca cccgtcgtta gatcaacagc accatattga
    26401 tctaaaaaaa tatgtaatgt cgaatcaatc gaattcatgc ctgttggcca gcgggtcatt
    26461 tccacaggaa aagacactaa taataaagca taagcaatca ttgcagggtt aaaaggattt
    26521 tgtcccaagc caccatataa atgtttggca attggaatgg caaaaattaa accaataaca
    26581 cttaaccacc acggtgataa aggactgata gataaaccta ataacaagcc agtcacgact
    26641 gctgagccat cgcttaaata aaattcaaga ttttttttac gcagctttaa gcaaatgcct
    26701 tctaaaatca gcgcaatggc aacacaccac aacacattaa tgattgagcc aataccaaaa
    26761 ctaaaagtta acgctgctaa ggcgggtaaa gtagcataac acacttggcg catgacccat
    26821 gcagtgcgtt gttgaccatg ttgatgtggc gaggtgaccg tgataaagga cattgattaa
    26881 atccttgatt tttaggcttt tgctttaatg tagattataa cgaaatgaca agtttggtgg
    26941 tagattggag tttaatgaat agtattagac aaagtttaga tgttgagacg gtaactgatg
    27001 attattcgtc taagccttaa aaccatttaa ttgattcata gctttgttta gattgccatt
    27061 aatcattaaa tcggtgactt tgacagcagc aataatcgca gattcgattt tttcttgttc
    27121 ggcagcaggg gctttgccaa gcacataatt agacactaat ttagcatcac cgggatgagc
    27181 aataccaatg cgtaaacggt gaaaattatt ttgattacct aaaccactaa taatatcgcg
    27241 taagccatta tgaccaccat gaccaccgcc agtttttaaa cgaatgctac ccacaggtaa
    27301 atctaattcg tcatgagcga ctaacatggc atcaggagat attttgaaga aattggctag
    27361 cgcagaaacg gcttggccag aacgattcat aaatgtggtg ggaattaata aacgaacttc
    27421 ctgtccgtta atttgaccgc gcccgactaa accaaaatat ttgctttcgg gggtcatgcg
    27481 gatgttatat tgttctgcca aggcagccac ataccaaaca cccgcattgt gacgagtttg
    27541 cgcatattct aaacctggat tacccaagcc aacaattaat tgcaaacccg ccatcgctta
    27601 gttcacctta agctttgtga acgttagcga ttggttggtc atagtcatga ccttgtgcta
    27661 ataaaggaat ctctacacct gctggtaaag caatgtcaga taagtgtaaa gttgtaccaa
    27721 tatgtacagc agcaaagtca acagttaaga actctggtaa gtcttttggt aaacaagcga
    27781 tttcaacatc gttagcaata atagaaattg caccaccttc agcttttaca ccaacacaag
    27841 attcttggtt gaggtagtgt aaaggcactt tcatggtgat tttgtgtgtg gcatcaatgc
    27901 gtaagaagtc agcgtgcata ggaatgtttt tagcaggatg acgttgtaat gctttgataa
    27961 cagcttgttg ttcttgacca tcaatagata atgtcaaaat gtgagagtaa aaagcttcgc
    28021 tttctaaggc tttaaccaaa tctttaaatg gaatagcgat agattgaggg gcttgacctg
    28081 cgccataaat aacagctgga acttgttgag cgtggcgaag gcggcggctc gcacctttcc
    28141 cttcgtcttt acggctaaca gccgttaaat tgaagttagc ggacataata gtctccaaaa
    28201 gttcagtatc ttgcgaccag atactgaggt tattagtatc acctaaacgt gatacttaga
    28261 aaaacaggca cttaaaaagt acctgagaaa atcgttttat gattagaata aatcaaacat
    28321 tgcgctgata gattcttcat tattaatgcg acgaactgtt tcggctaaca atggggctaa
    28381 agacaattga cgaatacggc cactggctac tgcctcagca gataaaggaa tggtatccgt
    28441 gactactaat tcatccattg cggagttaac aatatttttg attgctgcac ctgataaaac
    28501 agggtgggta caataagcaa ccacacggcg tgcaccatgt tcttttaagg ctgctgctgc
    28561 tttgcacaat gtaccagcgg tatcaaccat atcgtcaaca ataatacagt cacggccaga
    28621 tacgtcacca atgatgtgca tcacttgaga ttcattagca cgggcacgac gtttgtcgat
    28681 aatggctaaa tcaacttcac ctaactgttt agcaaccgca cgagcgcgaa ctacaccacc
    28741 tacgtcaggc gaaacgatca ttaaattatc aaacgattgt ttttgtagat cggctaataa
    28801 cacaggcgaa ccgtaaatgt tatcaacagg aatatcaaaa aaaccttgaa tttggtcggc
    28861 gtgtaagtca accgttaata cacggtcaat gcctaccgag gtaatcatat cggcaactac
    28921 tttggcagta atcggtacac gtgcagagcg tgggcgacga tcttggcgtg cataaccgaa
    28981 gtaggggaca actgctgtaa tacggccagc acttgcacga cgtaaagcat cagccatgac
    29041 aattaattcc attaaattgt cattggtagg tgcgcaagta gattgcaaaa taaagacgtc
    29101 tttaccacga acattttcat taatttcgac agaaatttcc ccatcagaga agcgagttac
    29161 ttgtgctctg ccgagaggaa catgtaaacg gtcaacgact ttttgtgcga gttcaggatt
    29221 ggcattgcca gtaaagacga ccaaattagg cattttgctt tacactatca aaaagtagca
    29281 ttaacaagat aacaaacggc tgttaaatgt tgattgctat taataataag aagatggcag
    29341 gggtggctgg attcgaacca acgcatggcg ggatcaaaac ccgctgcctt accgcttggc
    29401 gacaccccta tgtatagtgt tgcagtttat atgcttggta gcatgtactg catttcggag
    29461 gcgcgtaatt tacttcttat atgaaaaggt gtcaacatat tttgataaaa attaataaga
    29521 taattattta atagcctgat atttgagcaa tcggaaatga gtaataattt tagcttaaaa
    29581 atactactaa tttgataatg aacgtttgat aaataacttg aagatggcag gggtggctgg
    29641 attcgaacca acgcatggcg ggatcaaaac ccgctgcctt accgcttggc gacaccccta
    29701 agagagatat ttttgcaatg ctttgtgtgc aggcgagaga ttgtagcctc ttgttacaaa
    29761 gccgtcaact gttgattttg ctaatatttt attggcttgt tcaacagttg agcagtcaac
    29821 aaagatacaa caacctgtgc ctgtaagttt ggcattgcca tattgtctta accacaatag
    29881 tgcgttttga acaggaggat atagctcgct gactatcgct tcacagtcat tttggccatg
    29941 ctgtgcagcg tggggcgaaa ctgtaatagg cggtgtattt cttgtcaatg ctggatgggt
    30001 aaaaatctca cgagtgccta ttttgcaatc aggtttgagt acaacataga caggttcggg
    30061 taactctagg ggggttaaag cctcaccaac accttctgcc catgctgcac gaccataaac
    30121 aaaaattggg acatcagcac ccaaagataa acctaactga gccagttttt catttggcca
    30181 gtgcaaattc cataaaatgt ttaatgctaa tagtgtggtg gccgcattag atgagcctcc
    30241 ccctaagcct gcacccatag gaatgatttt gtttagatga atatctacac caagctggct
    30301 ttgactttgc tgttgtaaca ggcgtgctgc tttaacaatt aagttgtctt gactagcaat
    30361 atcagccaaa gcaggactta gggtaatcat gccatcttca cgcagcgtaa atgttaaggt
    30421 atcagcataa tctacaaact gaaaaatcgt ttgtagatta tgataaccat cggcacgttg
    30481 tcctgtgatg tgcaaaaaca aattgagttt tgcaggcgag ggtaaagtga gcgtagatag
    30541 cattattgtg tcgcgactac ttgaggttgc cagtcactaa ttaacaccgt catattaaca
    30601 ttggggccag tgacgacaat tttttgtggt aacagttggg tgtttacata acgataattt
    30661 ttaaaatctg cttgccagtt gccttcgtgg acactttcga gttggccact aggggcatag
    30721 tttaaaacag cagttggcgt agctgcacgg ccttcaagcc aatatttcat ataagaaaca
    30781 ggtgctgtcc aaccaaaagc ttgttcaaat aaagtttctg gatcatctgc gctcatttca
    30841 cctgtttgcg cgctttgcat aatcacgccg ttagcattac ccactaaaga ggttcgacct
    30901 tggcctaaag ggcctgtcaa aataatgtga tagtcttgag tgtcttgttg ccacaaaaag
    30961 taagcactgc cgcctttctt tttgcctgtt tggtcaatca tttttaccga aaatttacca
    31021 gaagctgtcc acatactttg tgcaggtaat tcaggtaatt gaatggtatc aacatcttca
    31081 ctaacagtca ggttagttaa agggtgagca gcaacaacag gtgttgatgt tgggattttg
    31141 tatggttctt taaaggttaa aatagtcgat tgactggtac aaccctgtaa taggattaac
    31201 gttgttgcag taaaaaaatg tctcacgctt catttctccc attgcgcgcg atgcttcaaa
    31261 acggcttcac tgtttggttt ttgcatcaaa agttcctgcc aaattttagc agcttctgct
    31321 ttaaaaccgc aaacccataa aacttcagct aagtgtaacc caatttcttc gtctttggat
    31381 gcggcataag cctttttaat atacgtcaac gcttcaaata agtggccttg tttgtacaac
    31441 aaccaaccaa cactatccat aatagcggga tcattgggtt ttagtttaat cgcttcgcgc
    31501 aaataacgat cagcttcttc aagtcgattt gttctgtctg ctaaggtgta accataagca
    31561 ttgaggtaac taggattttt gctgtctctt ttaaaaagtt cgagcatatc tagttcaaac
    31621 cgaattaaat catttcgctt ttcggctaat aatgcacggc taaataataa cagattgtct
    31681 tgaggaaatt tgttgatggc atcggcaaga acagcataag cttcatctaa tcgttgatga
    31741 tctcgcaaaa tttcggcttc aacagcataa agtgtattag caatttcggg gtgttgttgt
    31801 cgagattcat taaattgttg gcgcatttgc acatcacgtt gatctttaat tcttaaactg
    31861 gctaattcaa cttgtgcact taaaaaacga tcaccatcgt ttatttggct aaaataagat
    31921 tctgcttgac tcaaattatt ttgtcgtttg gcaatcatcg ctaaataata aaaagcttcg
    31981 tcaacattac tatttaaaac aatgagttta ttaaaacttt taatggcttg ttcatcatgt
    32041 tggttatcaa aagataacaa ggcatgcatg agtaacattt ctgtattttg aggctgtctt
    32101 agtagttgtt gcgctagttt ggtttcggca gcgttatatt gtttatcatt aattaaagct
    32161 cgtatgtagt gcatgttaag ctgaggacta ttaggtgttt tttgtaggct ttgttctagc
    32221 agtttaatgg ctttatcgct ttgtttattg agtgtcaaca atcgggcttg taataaaaca
    32281 gtgggaatac tacgcggatt gagcttttga gctttttcaa taaaaattaa ggcttgttta
    32341 tagtcacctg tttctccgac caataagcca tgtgcaaata gtagatgagg attatcagga
    32401 taatttttac ttaaattttg aatcgcgtct aaaagttgtt tgcgttgttc gggaaggctt
    32461 gggtaagcac ttaaaaaaag agcttctaag tctgattcag gctctagtaa taatagttta
    32521 tctatcgctt caatggcttg attatatttt tgttttttga gcgcatgata agccagctga
    32581 taataagcct tttgatcttt aggcgttgtt tctatccaga gcttggctaa atttaaacct
    32641 tgttcattat cttgtaaaaa gttggcaatt tgtaatgctc gttcaataat ggctttatct
    32701 ttagtttgtt gtgcttgttg actatattgt tccaaagcaa gattagtttg gttgcgttgt
    32761 agagccattt cgcccagtaa taatccatat aaactgtcat aactcaagtt atgattattt
    32821 tgcggtgtgt ttgcataact gaagttggct aatgtgagag tgatcaacgc tgtcgtacga
    32881 taaattttaa ccaatgtttt gttcatggta atggtgttaa tcccgtttta agtaacttgc
    32941 ttggctgact ataaacaaaa atgttgccct tgtttgtaat ccgtttgaaa aaaatcctgc
    33001 tttcgcgcac aataaccacc ccctagctaa ttgttgtttc ccttttatgg gttttatcgc
    33061 gctcggcatt aatcataaaa cagcttctgt gcatttacgc gaaaaagttg cgtttttgcc
    33121 agagttttta cctcatgctt tgcaggtggc ttgtgctcgt gcagaaatta atgagttggc
    33181 gatagtttca acctgtaatc gtaccgagtt ttatggtatt gccaatgata gccaaaaatt
    33241 attgcaatgg ttaagtcaag agcgacaaat ttcattaaca gaattacaaa catctattta
    33301 tttacatcaa gatcaacaag ctttacgtca tttgatgcgt gtagcctctg gtttagattc
    33361 aatggtattg ggtgagccac aaatttttgg ccaagtaaaa gttgcttatc aacatgctca
    33421 acaagcagga acagttggct ctcagctaga tcgaattttt caacaggctt ttgctgcagt
    33481 aaaacgtgtt cgtaccgaaa cggcgattgg tgcaaatcct gtgtcgattg gttttgcggc
    33541 agtgcaatta gccaaacaaa tttttaccga ccttaccaaa gctaatgctt tattggttgg
    33601 ggcaggtgaa atgattagct tggtggctaa gcatcttaaa gaacagggga ttaaacacat
    33661 taccattgcc aatcgcagtt tagaacgtgc tgaaaattta gcgactgaat tgggtggcgc
    33721 aaaagtcgtg ttattggccg atttggccga ggttttgccc caagcagata ttgttattag
    33781 ttgtacaggt agtcagttgc ctgtgattgg taaaggtatg gttgagcgcg ccttaaagaa
    33841 gcgtcgttat cagccgattt ttatggttga tattgctgtt ccacgcgata ttgaacccga
    33901 agttgatgat ttagaagatg tttatttata taccgttgat gatttagaaa gcaccattga
    33961 agaaaatcgt cgcgcccgtc aacaagcagc acaacaagcc gaacaattaa ttgaacaatg
    34021 tgccatagaa tttggccatc aacaacgtgc ccaagctgcc agtggcttgg tgatggccta
    34081 ccgtcaacaa gttgaagagt tacgattagc cgaaattgct aaagcacaac aatccttagc
    34141 ccaaggtgtg aatcctcatg acgtattaga acgtttatct cgtaatttga ccaataaatt
    34201 gatgcatacc ccgagtgtcc aactcaagca agcagcagca aacaatgctc atgataagtt
    34261 aatgtgggcg gctgagttgt taggtgtgca gcaaaacgaa tagtcggtca aacaacttat
    34321 ctgtatctta taaaatttct atacactccc tagtgattgt ttactaacga gacacgaaaa
    34381 aattttgagt taattatgaa agcatctatc cgtagtcgct tagaacagtt aagcgaacgt
    34441 tatgaagaag tgaccgcctt attatcagac agtagcacga tcagtgataa cgaaaagttt
    34501 cgtaaattat cccgtgaaca cagtgagtta ggtgagatta ctcaagtatg gcaggcattt
    34561 tgtcaggctg agcaagattt ggccactgca caagagatga aagctgatcc agagtttaaa
    34621 gaaattgctg aagaagagat tgatgcagct ttaaaacgta ttgatcaatt aaccgaacag
    34681 cttaatgtgt taatgattcc taaagaccct aatgatacct gtcctgtgta tttggaagtt
    34741 agggctggaa cgggtggtga tgaggcggct atttttgcag gcgatttatt tagaatgtat
    34801 tctcgctttg ccgaacgtca aggttggcaa gtagaagtga tctcttgcaa cgaaggtgag
    34861 catggcggtt ataaagaagt gattagccgc gtcagtggtg aaggtgtata tggcacgctc
    34921 aagtttgaat caggtgcaca tcgagtgcaa cgtgttccag aaacagagtc acaaggccgt
    34981 gtgcatacct cagcatgtac ggtggcggtg atgcctgaac ccgaagaaca agctgctatt
    35041 gaaattaatc ctgccgattt gcgtgttgat acttaccgtt cgagtggtgc gggtggtcag
    35101 cacgttaaca aaactgactc agctattcgt attacccaca ttccaactgg tattgtggta
    35161 gaatgtcagg aagaacgttc gcaacataaa aaccgtgcta aagcaatgag ttggttagcg
    35221 gctaaattag agtctcgtca acgtgaagca caacaacaag ccaccgctac tatgcgtcgt
    35281 gatttggtgg gtagtggtga tcgttcggaa cgtattcgta cttataattt ccctcaaggt
    35341 cgtttaacag atcatcgaat caatttaacg ttgtatcgtt tagatgcagt gatggaaggt
    35401 gatttaaccg aagtggttga ggctttagca cgcgaatatc aagctgacca attggcagca
    35461 ttagccaatg atgaaagcta atctgatatg actttaagca ttcaacagtt ccaacacctt
    35521 gcccgtcagc gtttaagtaa cgaagatgct gctttggaaa ttgagttaat gttgtgccaa
    35581 gtgcttaata aaaataggat ttttttgcgt acttggccag atacattatt aactgagact
    35641 caattagaac agcttaatga aaatttgcaa caacgtgaac aaggtgtgcc attagcctat
    35701 ttattgggtg aacgtgcttt ttggacatta aatttaacgg ttagtcgcga agtattaatc
    35761 ccacgaccag atactgaatg tgttgtcgaa aaagtcttgg ctttggggca gggtaaacag
    35821 tggcgcgtgg ttgatttgg
//
LOCUS       DAOQMB010000002        22742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_774_length_22742_cov_14.684753, whole genome shotgun sequence.
ACCESSION   DAOQMB010000002 DAOQMB010000000
VERSION     DAOQMB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 22742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22742
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_774_length_22742_cov_14.684753"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..1033
                     /locus_tag="PLY05_00205"
     CDS             38..1033
                     /locus_tag="PLY05_00205"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNC01752.1"
                     /translation="MRLLALLFSFILLVACNQSDSAVNQATNPAKSAVQEDLSQLPKS
                     PESFEKAKRILYNDIYKGHNITFYCGCDYDPKSKLVDWKSCGYVPRKNPERASRIEAE
                     HVMPAHQFGNFRQCWREPKKVCPEKEMTGRQCCEAKDPVFETAHNDLHNLFPAVGEVN
                     GDRSNFNWGMVEGSKREYGACPIEVDESIRRAEPPDAVKGNVARVMFYMEDTYGFKLS
                     DQDKKLYSVWSKQDAPDEWEIERNQRIAAIQGKDNRFISGYGNKKQEVVADLKTEPKT
                     AEPKTKATATEFSCNTKKTCSQMSSCAEAKFQLKTCGNTSIDGDGDGTPCASLCK"
     gene            1048..1518
                     /locus_tag="PLY05_00210"
     CDS             1048..1518
                     /locus_tag="PLY05_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNC01753.1"
                     /translation="MKLLVCVDLSTSSEKVIDYVQNFAKKLVQASICVLHVTDSQDEV
                     LGYGGVFGEYPVYIDPKELRQEIATRFKHEHQKVQEISHRLQQSGLKCLGLLVNGDSV
                     VQTILKEATKHQADMVIVGSEHKGLLMQLVEGSTSKSLINQASIPVLVVPVKTL"
     gene            complement(1559..2020)
                     /locus_tag="PLY05_00215"
     CDS             complement(1559..2020)
                     /locus_tag="PLY05_00215"
                     /inference="COORDINATES: protein motif:HMM:NF040030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6231 family protein"
                     /protein_id="HNC01754.1"
                     /translation="MWPDITPLLQQLKKECSDKAWLITGKNLNCPLAEVDLTHTTVNY
                     MSSEQLLTAPFTQRFAAALMLLDEQDDFSQGTQVISKLRDLLAERVLVITSDQSTTWP
                     TNIMLGFGFKRIAELKGYQAWGFDIHTYKEVPDWLNAKFWANPQNWGKYWW"
     gene            2065..2850
                     /locus_tag="PLY05_00220"
     CDS             2065..2850
                     /locus_tag="PLY05_00220"
                     /inference="COORDINATES: protein motif:HMM:NF022430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HNC01755.1"
                     /translation="MRTQVSSPIRYELVIGGMILPLNDYLGKNINLNYTGRIFCLNCG
                     QKTSKSFNQGYCFKCFRSSASADMCIMKPETCHYHLGTCREPEWGETHCFMPHIVYLA
                     NSSGVKVGITRKTQVPTRWLDQGAMQALPIFEVSNRRLAGLIEVTLAQYIPDKTQWQR
                     LLKNDAETLDLAFVKDEVLTLCKKELLALANQFPQQMRVLDNQSYDFSFPVLQYPNKV
                     KALNLDNQAQISGVLQGIKGQYLILDTGVINIRKFGGYEVEFS"
     gene            complement(2829..3305)
                     /locus_tag="PLY05_00225"
     CDS             complement(2829..3305)
                     /locus_tag="PLY05_00225"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005215259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC01756.1"
                     /translation="MPLNIGDSIPNLVVNTTHQEQISLGELSGKNCVIYFYPKDSTPA
                     CTTEAQNFRDLMPEFAALNCTILGVSRDSIKSHQRFTANECLAFELISDTDETVCQAF
                     DVIKLKNMYGKQVLGIERSTFLFINGQLRHVWHKVKAQGHAQAVLDTLKNHENSTS"
     gene            complement(3323..3853)
                     /locus_tag="PLY05_00230"
     CDS             complement(3323..3853)
                     /locus_tag="PLY05_00230"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein R"
                     /protein_id="HNC01757.1"
                     /translation="MDNHLVLAALGADRTGIVDEISRIASETRCNITDSRMVVMGQEF
                     SIIMMISGAWDAIAKFENHIPVLEQRLNLAIITKRTEARQPQREAIPYRVDVIALDNP
                     GIVHDIANFFASQQINIESLETSTYAAPHTGSPMFALNMTVNIPAKAHLASLRENFMI
                     FCDDRNLDAILEPYKH"
     gene            complement(3891..4571)
                     /gene="queC"
                     /locus_tag="PLY05_00235"
     CDS             complement(3891..4571)
                     /gene="queC"
                     /locus_tag="PLY05_00235"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNC01758.1"
                     /translation="MTLSNKPRAVVLLSGGLDSATCLAIAQQTYDCYALSFAYGQRHN
                     SELAAASRLAIALNAKEHRIINIDLGGIGGSALTDTSIAVPEEASQGIPVTYVPARNT
                     VFLAFALGFAEVVGADAIVIGVNAVDYSGYPDCRPEYIAAFQQLANLATKAGVEGHPL
                     NIETPLLHLSKAAIIQQGTQLGVDYALTVSCYQADLDGKACGKCDSCRLRKEGFLAAG
                     LADPTRYR"
     gene            complement(4573..5247)
                     /gene="queE"
                     /locus_tag="PLY05_00240"
     CDS             complement(4573..5247)
                     /gene="queE"
                     /locus_tag="PLY05_00240"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004948660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HNC01759.1"
                     /translation="MRLATVTDSLLAELRITEIFFSLQGEARTVGLPTVFIRLTGCPL
                     RCSYCDTTYSFTGGEKYTLTAIMQQVADYGAKYVCVTGGEPLAQPNCLPLLTALCDAG
                     YQVSLETSGALDVSQVDRRVSKVVDLKTPDSGEAHRNLYENMQHLTANDQIKFVICSR
                     QDYQWAKFKIDEYQLNQRVSDVLLSPSHQQQDPRELAEWILADKLAVRLQVQLHKYLW
                     NNEAGH"
     gene            5387..5728
                     /locus_tag="PLY05_00245"
     CDS             5387..5728
                     /locus_tag="PLY05_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC01760.1"
                     /translation="MESLAIKNLDNNKLVVEVQKKRLDAAFAVKFRDDILSLVQQGHN
                     KIIFNLSQVEFMDSSGLGALVSVMKALGGNQNMVLCGVKDAVLTVFKLTRMDKIFVIT
                     ASEPDAISRLS"
     gene            5776..6255
                     /locus_tag="PLY05_00250"
     CDS             5776..6255
                     /locus_tag="PLY05_00250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC01761.1"
                     /translation="MMSVHDSQIKLTIRARLPDVSTLGVTIRVLADEFGLEMMACTEI
                     ELAVVEAVTNVVKHGLNKSPDALIEITLYSTDQLLAITIFDQGIPMPADALDAADGSV
                     FDYIAEDIEGWPTSGMGLSLIKAVMDEVQYSHDETGNTLRLIKKKLITESTHVQADN"
     gene            6299..8470
                     /locus_tag="PLY05_00255"
     CDS             6299..8470
                     /locus_tag="PLY05_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HNC01762.1"
                     /translation="MTDISLAGDWWYQTANLKPADIYLPSSKSHLQRDSWQKITIPNN
                     WYSAGLDFSGAVWHKKNFNLGDNLDTKQATLHFKAVDYAADVWLNGQYLGFHEGYFEP
                     FNFDVTGLLKKQDNLLVVRVDSPYEPIEKVWSLKKRQIKGVLNHHDTRPAGAWSVNGQ
                     DANSGGIWQEVWLHFSDQLSIQHLTVEAVIKDAILTDKVDNLALQIQLQLSMAQPQAL
                     KAKIAIEQINGKDNKQPVNIDIDASQLVKVSENQYQISQSVLVNQLKTWWPQGYGESA
                     VYRLKIDLLDQKNQLLDSINQRFALRTIRYDDDQFAFYINGRRLFLRGTNYIASPWLG
                     EVKRQDYQRDLVLMQQANINAVRVHAHIAGQDFYELADELGMLIWQDFPLQWGYDDSP
                     EFANEAVRQAKAMLTVLHHHPSIFAWSGHNEPPWNAPWMKYKYPDYKPQQNRYLTDAV
                     GAVLASDKTRYSHDYSATDEHLWMGWYSGNWKEHAKRSKVTIVSEFGAQALPDVARLK
                     KIIPASDLWPRTLDAKDPMWKSWDYHNFQVQETFKNAGISRGDSLESFVKNSQEYQAK
                     LTQLAGESYRRQRYQPVAAMFQFMFNETWPSINWGVMDYTRQAKAGYWALKQAYQPVL
                     ASIEWDVDKLKTNDEACFVLWSINDTWQDYPSLKLRYRLLLANKVVKEGQKEINLMAD
                     EAHPEWELKWRGLSQGSYRLEATLSQQKKIISNNSFEFVVE"
     gene            8498..10303
                     /locus_tag="PLY05_00260"
     CDS             8498..10303
                     /locus_tag="PLY05_00260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC01763.1"
                     /translation="MAFYFEKFEHRKPPEPVPHRPYIELVWQFLAVIALVLGGNYIYW
                     RWTASLNYDALWFAIPLAFAETCAYFGLFLFAFNLWKVKDYPQQEPPKTISDCLAHAL
                     DDHEDRPVSVDLFIATYSEEEELVRLSIQDAKKVNYPHHIDYKIFVLDDGRRASMKAV
                     CDEEGVGYITRSNNIGFKAGNLRNAMEKTHGDFIVICDADTRVFPTILEDTLGYFRDP
                     KVAWVQTPQWFYDIPEGKPLPDVWQKYAGRLGYYAGRGLEKVVGPIQLGRDPFVNDPQ
                     MFYDIIQRRRNWANASFCCGAGSIHRREAVMQGALRAYANAIEKEVHHQTKDVDDAEL
                     RETLQDAIRTQAVMDTELTPYKFHVSEDIYTSIVLHSDPEHHWKSVLHPKVESKMLSP
                     QDLQTWMIQRFKYAGGSLDIALRDNPVFQGNLTWPQRVMYMTTFWSYLGCIWNVIFLV
                     APTIFLITGISPVSAYSTPFYWHFLPFIICTELAFMFATWGVKAWDGKASYLSFFPVN
                     FQALANVLRGEKIKFPVTPKERQEGTFIHLVYPQLAIIVLSIIGLVIGFIKMALDMPL
                     DVSGFLINVFWSINNIAAMYPLVRAALWKPDYQVA"
     gene            10318..11877
                     /locus_tag="PLY05_00265"
     CDS             10318..11877
                     /locus_tag="PLY05_00265"
                     /inference="COORDINATES: protein motif:HMM:NF022773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HNC01764.1"
                     /translation="MALKDDLITARSYIVFVLGLLVAFSMVYYIEQKATVAPQVIPPV
                     MMVKSVVNLPNNQVQATPVVQSERAIDEQVIDKPVPVVAVQTVVAEETEKKLDAPVVT
                     AQVSTIQPQQMVADNAMKPKVPDDVVLNPLMVKKRQPRVLTAEEMEWARIAWQYFANN
                     TQATGLVNSVNGYPSTTMWDTASYMLGAISAQRLGIISKKELNERMANVLVALEKIPL
                     FDGKLPNKSYNTKTLAMVNYANKDATKGIGWSAIDIGRLLVPLKIIYQKFPAHRPSIN
                     KVLARWQTQNMLDRGLLFGASLKADGTVHLLQEGRLGYEQYAAKTFALFNNWSANAAA
                     SYTNYLEYVIIDGIKIAIDKRDPKQHGAYNYMLSEPFMLDGLEFGFDKNSAQLAEKMF
                     LVQEQRYKRTGIVTAVTEDHIDVAPYFVYNTVYVAGKSWAAITDKGQDASAFRALSTK
                     AAFAWYVLYQHNYSDKLINAVKGLNVANQGWYAGLYEEGKRPNKVITANTNGVILESL
                     AYLKSGKLLAN"
     gene            11890..13470
                     /locus_tag="PLY05_00270"
     CDS             11890..13470
                     /locus_tag="PLY05_00270"
                     /inference="COORDINATES: protein motif:HMM:NF022773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HNC01765.1"
                     /translation="MIKAKALTLCLAMLANASVVVSAQAMTVSAQPPIAYKQRQGALT
                     ETEMEMAKIAWRYFQNNYQPETGLVNAVDGYPSTTMWDTASYMAALVAAHQFNFIDKQ
                     TFDSRLTKLIATLNNLVLFRNELPNKVYHTKTAEKVNYANKAGEIGFSALDLGRMLIW
                     LRILKENYPEHANAIDSVVLRWDFSNVVDKCGTMYGAMLNADKKTVYIQEGRLGYEEY
                     AAKGFQLWHIPTCLSTRPEPFAVMPMYCINVPYDTRDPRELNQHNYVVSESYVLDGVE
                     LGWDIVGDMHSADNVFTDRIMENFAHRVYQVQENRYKEAGIITARSEHQLDKSPYFVY
                     DTVYTDGYPWNTITDSGKYVPEFAAVSLKAALGMWVLWDTPYTDLLANHIWHQYVPEK
                     GYYEGVYENGKGPIKAFTANNNGIMLESLLYKVKGKLLTMNKATKPVVSLWEKTIADS
                     YNGENKKRNRPQICDMTLSDIKPLTEKKTWFFWPQKSTNAPIREFKPLNNECTVQKTC
                     SVCQEKAPVILPAWSSCH"
     gene            13419..14816
                     /locus_tag="PLY05_00275"
     CDS             13419..14816
                     /locus_tag="PLY05_00275"
                     /inference="COORDINATES: protein motif:HMM:NF022773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HNC01766.1"
                     /translation="MPRKSTCYIASLVILSLSLSSCGVVVRQANQLYQQIDAGSLLHQ
                     GRIAPLTSKEITWAKTAWRYFINNTDNETGLVNALDRYPMATPWTMADSLAALLSAHQ
                     LGLIEKIEFDQRLSRLLAFMSQMDLVEGQLPNRFYQTKTGKMLNQYSEVGEAGWSALE
                     ISRLLIWLKIVGEYYPEYHEYLDKIVLRWHFCQVIDDCGQLKLKTKANNQWQYESEGR
                     LGYQQYAGFGFSLWGFNSDKALNFNPNERIKVDNINLLYDARDPRLTHISNPILTTPY
                     NLLGLEFAWQIFPTEAAKQQKLFIEQAQMVYRVQEKRWQTEKIFTAKADYYRLTEPRS
                     LNDSVFANGQPWNTINDKGQWYPKLAVVSTKAAFSMWVLWKTDYTQQLMKVIEALYDK
                     DRGWYEGRYEASGAYERQITSYTNAMVLESLLYKAKGPIYRQVNQTRHFNQRLDKIYA
                     RPQQCFPQERPVCPL"
     gene            14845..15723
                     /locus_tag="PLY05_00280"
     CDS             14845..15723
                     /locus_tag="PLY05_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01767.1"
                     /translation="MALSLNISLSHAATISNNDYYQTGWAFYRAGQTAEAAKTWQALA
                     SSSEQQSDEQKKQAALSAVFATVLWQNLENPQAYNTWSDAIRLYLEANSSWEQERVML
                     KQRIATVRQALQQLSGDVVPTIEPFMSVLLEMDSRYGLTEFQTPKSGLKLTNPDSLLE
                     LNQNFLASPPVVINEENVSLTTTVDSSSSEMQPTSPQGESNNEQTVQTLTNQQHFAVD
                     TQSETVKPTNDQQDKSDKEQANNENNTQTLPVEAEIELTTPERGHLIKPEETNVNEVN
                     VEGTAFQRHVTPTPEE"
     gene            complement(15736..18591)
                     /locus_tag="PLY05_00285"
     CDS             complement(15736..18591)
                     /locus_tag="PLY05_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC01768.1"
                     /translation="MIETKLLKRQLRRLFDIQDVSNLDFITQELSDLKHHTTSHTVHK
                     LLDNFSSFLQHVDEAYQQSARDLELRSRSLEISSQELNWANQRLANELALQQQTINNL
                     KQLAERLSKKIGVDYNGLDERLDSVSLLLNKLVDSYQELSDVLKLSEERFQLAVASAE
                     IGLWDWQIDSDSIYFSQEWLDLLDINPSKNNHFALWYDYLHPHHTSIFEQQISALITK
                     SSHYLNLEVQMRHSSGEYIWVQIQGKVQNQDSPQRRCLGTMVNIHARKIAEAAIIEAK
                     EAAEASNQAKSDFLANVSHEIRTPMNGIIGMTKLCLDTPLNAEQEEYLQMVSSSAMSL
                     LGLINDVLDFSKIEAGKMVIDPTDFNVRKLIRDTLRPLALRAQEKSIELTCDIQNQVP
                     VSLITDANRLRQVLINLLGNAIKFTETGEVGLKIAITCHTNQQNELFVCVSDTGIGIE
                     PHLLDKIFESFSQGDSSITRRYGGTGLGLAISSKLIELMGGKLTVKSQMGVGSEFSFS
                     IPVTIGHEQEVSPVTPDTLKKLPVLVVDDNSTNRRLMYDMLHNFGMDPFVVGDARSAM
                     MKMVDHALEGTPFNLVLLDAQMPDRDGFSLAKDIITEKKLGSPRLVMLSSIAAPPDSN
                     TLKNLGIADFLSKPIDQSELYNCLLHVLGTVDKHKLQTADHKNNSLTTQSPRPQRVRT
                     LTANEPISNMEISDRLHILLAEDNTINQKLAIRLLDKLGYQCFLAKDGLEAVNAISSD
                     RFDLILMDIQMPTMGGIAATHHIRHWCEEKNHPHIPIIAMTAHAMTGDRERFLTEGMD
                     GYVSKPIMVDELKAEIDRVLLLYPPQHRNKIDMNTETIPTVCFDYELTLENMGGDPSF
                     VAELAQVFIKESPERMQLLKQAVVQRNAEQIYLISHKLKGESANFGRPLIEDIANKLC
                     EMGKKQQFTEIDETFAELEKQVAMFIADLEYRVLRIK"
     gene            complement(18588..19730)
                     /locus_tag="PLY05_00290"
     CDS             complement(18588..19730)
                     /locus_tag="PLY05_00290"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNC01769.1"
                     /translation="MNIKQIYTNNFELNSSQIAELKSIKPQLVLVFASISHFEHDTFI
                     KQLCTEFNEALLIGCSTAGEIYQDGVLDNSVVLTALYFDHPDFVLVKTSLKSMDDSFD
                     AGLRLAQQLQNHSLNNILLFGQGVNINGSALIEGLKSQLPSQTVISGGLAGDSGQFSR
                     TFILNGEHRSTEDILALGFYGTHIQLTSACFGGWKGFGTTRRITKHIGNVLYELDGKP
                     ALELYKRYLGDYADKLPASGLLFPFSMLSADGNDVGIIRTILGINEAEGSLILAGDLI
                     DGGYLQLMHASTDALIDGAEQAAILVNSLRQHQQAGLAILVSCVGRKLLIGGRIDEEV
                     DAVSDIFGEETIITGFYSNGEISPFSNTTDCKLHNQTMTITYLSET"
     gene            19964..>20305
                     /locus_tag="PLY05_00295"
     CDS             19964..>20305
                     /locus_tag="PLY05_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01770.1"
                     /translation="MTNKSMVLMLSILLMACQNQAIRQGPAPIIPKGQPFVEKDFDET
                     IAITPPQYIFPISPRLSLIPDFDFDGVNDEVDQCLDTPSNVKVDQKGCTIDPDDDKDG
                     IKNSVDKCPNTP"
     assembly_gap    20306..20405
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            20406..20637
                     /locus_tag="PLY05_00300"
                     /pseudo
     CDS             20406..20637
                     /locus_tag="PLY05_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            21271..>22742
                     /locus_tag="PLY05_00305"
     CDS             21271..>22742
                     /locus_tag="PLY05_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC01771.1"
                     /translation="MLANCDKQASKAKIKKIFLLSSICLLTACGGGGGGDSSPSTNTD
                     NNSNNNSELNQAPTNVAIKKASATSSSEVQIEWDSASDDKTIATDLSYEIHLSEGGDF
                     TVSDSTRKFSGKNILNAAITGLKAQTAYQVKLIAIDSQGLKTISSAKTVTTLNESTPP
                     INQAPTNVSLTKVTALTTTSLSIEWAVAKDDSTLPEKLVYEVHITEGKADFQPSLTTL
                     KKTLSNTLSTQLDELKVQTDYSVKLLVKDEQGLSTSSQVLFVTTLKADSVAVDKINDT
                     GITICANDNTIFSQCNVTNLGGWFNWQQDGQMGRDALAAQKQLKKIGIGVAGFDFSKL
                     GIKGELLSDNATMWSCVKDNLTGLIWEVKTNDKALRDTQNTYSWYNTDGTSNGGFVGK
                     QESNNTLDYTNKVNQSALCGFSDWRLPSRQELESILDYGQTTLATVDNNLFLNMQRKS
                     YWTSTTVAVSPASAWAINFADASDGQHSKSNMFPVLLVRGG"
BASE COUNT         7181 a         3902 c         4584 g         6975 t
ORIGIN      
        1 ttgtgtctaa atgttcaaca gatcctatga gtatcgtatg cgtttattag ctttactatt
       61 ttcatttatt cttttagttg cttgtaatca gagtgattcg gcagtaaatc aggcgactaa
      121 cccagcaaaa tcagctgttc aggaggattt gagtcagctt cccaaaagtc cagaatcctt
      181 tgaaaaggct aaaagaattt tatataacga tatttataaa ggccataata taacatttta
      241 ttgtggttgt gattatgatc cgaaatccaa attagtggat tggaaaagtt gtggttatgt
      301 tccgcgtaaa aatccagaac gtgctagtcg aattgaagct gaacatgtaa tgcctgcaca
      361 tcaatttggt aactttagac agtgttggcg cgagcctaaa aaagtatgtc cagaaaaaga
      421 aatgacaggt cgtcagtgtt gtgaggcaaa agatcccgtt tttgagacag cacataatga
      481 cttacataat ctttttccag cagtgggcga agttaatggt gatcgttcta attttaactg
      541 gggaatggta gagggtagta agcgagagta tggtgcttgt ccaattgaag tggatgagtc
      601 tattcgtcgt gctgaaccgc ctgatgctgt aaagggtaat gttgcccgtg tgatgtttta
      661 tatggaagat acctatggtt ttaaattatc tgatcaagat aaaaagctat atagtgtatg
      721 gtcaaaacaa gatgctcctg atgaatggga aattgaacgt aatcaacgta ttgcagcaat
      781 tcaaggcaaa gataatcgtt ttattagtgg ctatggaaat aaaaagcaag aagtcgttgc
      841 cgacctaaaa acagagccaa aaacagcaga acctaaaacg aaagcaacag caaccgaatt
      901 ttcctgtaat actaaaaaaa catgtagtca aatgagtagt tgtgcagagg caaaatttca
      961 attaaaaact tgtggtaata caagtataga tggtgatggt gatgggacgc cctgtgcgtc
     1021 attgtgcaaa taaataggag tgcaatcatg aagcttttag tatgcgtaga tttatcgaca
     1081 tcttctgaga aagtgattga ttatgtgcaa aattttgcaa aaaagcttgt acaagcaagt
     1141 atttgtgttt tacatgtgac cgattcgcag gatgaagttt tgggatatgg tggtgtcttt
     1201 ggtgaatacc cagtttatat tgaccctaaa gagttgcgtc aagaaatagc gactcgtttt
     1261 aaacatgaac atcaaaaagt tcaagaaatt agtcaccgtt tgcaacaatc aggcttaaaa
     1321 tgtttgggct tgttagtcaa tggcgattct gtggtacaaa ctattttaaa agaagccaca
     1381 aagcatcaag cagatatggt cattgttggt tctgaacata aaggcttgtt gatgcagtta
     1441 gttgagggaa gtactagtaa gtctttgatt aatcaagcaa gtattccagt tttagtcgtg
     1501 ccagtaaaaa ccttataatg gctttttgtc gtaatctagg ggaattctcc tagattactt
     1561 accaccaata tttgccccag ttttgaggat ttgcccaaaa tttagcgttt aaccaatcag
     1621 gaacttcttt ataggtatga atgtcaaagc cccatgcctg ataacctttt agttcagcaa
     1681 tacgtttaaa accaaaacca agcataatat tggttggcca agttgttgat tggtcagagg
     1741 taatgactaa aactcgctct gcaagcagat cacgtagttt gctaatgact tgtgtacctt
     1801 ggctgaagtc atcttgctca tctaataaca taagtgccgc agcaaaccgt tgtgtaaaag
     1861 gggctgtcag taattgttca ctactcatat aattaacagt agtatgggtt aaatccacct
     1921 ctgctaatgg gcagtttaag tttttgcccg taataagcca tgccttatca gagcattctt
     1981 ttttcaattg ttgtaataac ggcgtaatat caggccacat ataggtttaa ctcagttatg
     2041 ttgtaccaag gtgttttgca aaaaatgcgt acgcaagtta gttcaccgat tcgctatgaa
     2101 ttggtaattg gtggaatgat actcccttta aatgattatc ttggtaaaaa tattaattta
     2161 aattatacag gacgtatttt ttgtttaaat tgtggtcaaa agacaagtaa gtcttttaat
     2221 caaggttatt gttttaaatg ttttagaagt agtgcttctg ccgatatgtg tattatgaag
     2281 ccagaaactt gccattatca tttgggaacc tgccgtgaac ctgaatgggg cgagacgcat
     2341 tgttttatgc ctcatattgt ctatttggca aattcatcag gtgtaaaagt cggtattact
     2401 cgcaaaacgc aagtacctac acgttggtta gatcaaggtg ctatgcaagc tttacccata
     2461 tttgaagtat ctaatcgtcg tttagcggga ctgattgaag tgacattggc acaatatatt
     2521 ccagataaaa cccaatggca aagactatta aaaaatgacg cagaaacttt agatttagcc
     2581 ttcgtcaaag atgaggtttt aacattatgt aaaaaagagt tattggcttt agcaaaccaa
     2641 tttccacaac aaatgcgtgt tctagataat cagtcttatg attttagttt tccagttttg
     2701 caatatccaa ataaagtcaa agccttaaat ttagataatc aagcacaaat ttctggcgtc
     2761 ttgcagggga ttaagggcca gtacttaatt ttagatactg gcgtgattaa tatacgtaaa
     2821 tttggtggtt atgaagtaga gttttcgtga ttttttagcg tatcaaggac ggcttgagca
     2881 tgtccttgag ccttaacttt gtgccataca tgacgtagtt gaccattaat aaataaaaag
     2941 gtactacgtt caatgcccaa aacttgttta ccatacatat tttttaattt aataacatca
     3001 aaggcttggc aaaccgtttc atctgtatca ctaattaatt caaaggctaa acactcattg
     3061 gcggtaaaac gttgatgtga cttaatacta tcacgcgata cgcccaagat agtgcaattt
     3121 aaggcagcaa attcaggcat taaatctcta aaattttgag cttctgtagt acaggcaggt
     3181 gttgagtctt taggataaaa ataaatgaca caatttttgc ctgataattc acctaaagaa
     3241 atttgttctt gatgcgtggt atttacgact aaattaggaa tggagtcacc aatatttaaa
     3301 ggcatgatta atcctgtgat ttttagtgtt tatagggttc taaaatggca tctaaattgc
     3361 gatcatcaca gaaaatcata aagttttcgc gcaagctagc taagtgagct ttggcaggaa
     3421 tattaacagt catattcaga gcaaacatcg gtgagccagt atgtggcgca gcataagtgc
     3481 tcgtttctaa tgattcaata ttgatttgtt ggctggcaaa aaagttagca atatcatgaa
     3541 caataccagg attatccaag gcaatcacat caacacgata cggaatagct tcgcgttgtg
     3601 gttggcgtgc ttcggtacgt ttagtaataa tggctaaatt taagcgttgt tctaaaacag
     3661 gaatatgatt ttcaaatttg gcaattgcat cccatgcacc agaaatcatc ataataatcg
     3721 agaactcttg tcccatgacc accatgcggc tgtcggtaat gttgcaacga gtttcactgg
     3781 caatgcggga gatttcgtca acaatacctg ttctgtctgc acccaatgcg gctaatacta
     3841 aatgattatc catattttac ctttataatt tatttaaata atgcccgact ttagcgatag
     3901 cgggtcgggt ctgcaagccc tgcagctaaa aatccttctt tacgcaatcg acaactatca
     3961 catttgccac aagctttgcc gtctaaatct gcctgataac aagaaacggt taaggcataa
     4021 tccacaccaa gttgtgtgcc ttgttgaata atggcagctt tagataagtg taataggggc
     4081 gtttctatgt tgagtggatg gccttcaacg cctgctttgg tggctaaatt ggctaattgc
     4141 tgaaaagcag caatatattc gggacgacaa tcaggatagc ctgaataatc tacggcattg
     4201 acgccaatca caattgcatc tgcgcccacg acctcagcaa acccaagcgc aaatgctaaa
     4261 aatacggtat tacgtgcagg aacataagtg acgggaatac cttggctggc ttcttcagga
     4321 acggcaattg acgtatcggt taaggctgag ccaccaatgc cgcctaagtc gatattaata
     4381 attcgatgct ctttggcatt taaggcaatc gctaaacgac tagcagcggc taattcacta
     4441 ttatggcgtt ggccataagc aaagctgagc gcgtaacaat catacgtttg ttgcgcaatg
     4501 gctaaacagg tagcagagtc taaaccgcca gataacaaaa cgacggcacg gggtttgttg
     4561 gataaggtca tattaatggc ctgcttcatt attccagaga tatttatgga gttgcacttg
     4621 taaacgaaca gcaagtttgt cggctaaaat ccattcggct aattcacgcg gatcttgttg
     4681 ctggtggctg ggtgacaata aaacatcact cactcgctga ttgagttgat attcgtcaat
     4741 tttaaatttt gcccattgat aatcttgacg actacaaatg acaaatttga tttggtcatt
     4801 ggccgttaag tgctgcatat tttcataaag attacggtgt gcctcacctg agtcgggcgt
     4861 ttttagatcg acgactttac tgacacgtcg atcaacttga ctgacatcca acgcgccact
     4921 ggtttctaac gaaacctgat aacccgcatc acataaagcc gttaataagg gtaggcagtt
     4981 gggttgtgca agcggttcac cacctgtgac acaaacatat tttgcgccat agtcagccac
     5041 ttgctgcata atggcggtaa gggtgtattt ttcaccgcct gtaaatgaat aagtggtatc
     5101 acaataacta caacgtaacg ggcagccagt taaacgaata aaaaccgtag gtaaacctac
     5161 ggttcgtgct tcgccttgta aagagaagaa gatttcggta atacgtaatt ctgctaataa
     5221 agagtcagtc acagtagcaa gcctgatgct aaaatctaga ggctattatt ttaacatatt
     5281 cagctcgtct gtgtttaaat ccaattaaaa ggtcagcagc ccctattggt tttatagtgt
     5341 aggcagtgta gtatcattaa taataaattg gatcggagaa ttataattgg aaagtttagc
     5401 cattaaaaat ttagacaata ataagcttgt tgtcgaagta caaaaaaaac gccttgatgc
     5461 tgcatttgct gttaaattta gagatgatat tttgtcttta gtacagcaag gacataacaa
     5521 aataattttt aatttaagcc aagtcgaatt tatggatagc tcaggtttag gtgctttagt
     5581 atcagtgatg aaagccttag gtggcaacca aaatatggtt ttgtgtggtg taaaggatgc
     5641 tgtattaacc gtctttaaac tcacacgcat ggataaaatt tttgtgatta ctgcatctga
     5701 acctgatgct atatcacgat tatcgtagtt tgatatgcta gacataatta acgttaatgc
     5761 caacaaagga cagtaatgat gagtgttcat gactctcaaa ttaagttaac tattagggca
     5821 aggttgcctg atgtcagtac tttgggggtt acaatacgag tattggcaga cgagtttggc
     5881 ttagaaatga tggcttgtac agaaatagaa ctagctgttg ttgaggctgt cactaatgtt
     5941 gttaagcatg gtttaaataa atcaccagac gcattaattg aaataacgtt gtattctaca
     6001 gatcagttat tagcaattac catttttgat caaggaattc caatgcctgc tgatgcttta
     6061 gatgcggcag atggctctgt atttgattat attgctgagg atatagaagg gtggccaact
     6121 tcagggatgg gtttatcttt gattaaagcg gttatggatg aagtgcaata tagccatgac
     6181 gaaacaggca ataccttacg tttgatcaaa aaaaagctaa taacagagtc aacacatgtt
     6241 caggcagata actaagctat tcatattaaa tttagctttt ttagataatg tttggtctgt
     6301 gacagatata agcttagcag gagattggtg gtatcaaaca gccaatctaa aaccagctga
     6361 tatttattta ccgtcttcta aaagtcattt gcaacgtgat tcatggcaaa aaattactat
     6421 ccctaataac tggtattctg ctggtttgga tttttcaggg gctgtttggc ataaaaagaa
     6481 ctttaattta ggtgataatt tagacacaaa acaagctacg ttacatttta aagcggtgga
     6541 ctatgctgct gatgtttggt tgaatggtca atacttgggt tttcatgaag gatattttga
     6601 accatttaat tttgatgtaa caggattatt aaaaaaacaa gataatttat tagtcgtaag
     6661 agtggatagt ccttatgaac ctatcgaaaa ggtatggtca ttaaaaaaac gccaaattaa
     6721 gggtgtttta aatcatcatg atacgcggcc tgctggtgca tggtcggtca atgggcaaga
     6781 cgcaaattcg ggtggaattt ggcaagaggt ttggttgcat ttctctgacc aactcagtat
     6841 tcaacatttg actgttgaag ccgtcataaa agatgccatt cttacggata aagttgataa
     6901 cttagcctta caaatacaat tacaactgtc gatggctcag cctcaagcat taaaagctaa
     6961 aatcgccata gagcaaatta atggtaaaga taataaacag ccagtcaata tagatattga
     7021 tgctagtcaa ttggtcaaag tgagtgaaaa tcaatatcaa atttctcaat ctgtattagt
     7081 taatcagtta aaaacgtggt ggccacaagg ttatggagaa tctgctgttt atcgcttaaa
     7141 aattgattta ttagatcaaa aaaatcaatt attagatagt atcaatcagc gttttgcgct
     7201 aagaaccata cgttacgatg atgatcagtt tgctttttat atcaatggtc ggcgattgtt
     7261 tttacgaggc acaaactata ttgcctcgcc gtggttaggt gaggttaagc gtcaagatta
     7321 tcaacgtgat ttagtgttga tgcagcaagc gaatattaat gccgttcgtg tccatgcgca
     7381 tattgcaggc caagactttt atgagcttgc tgatgaattg ggtatgttaa tttggcaaga
     7441 ttttcctttg cagtggggct atgatgatag tcccgaattt gccaatgaag cagtaagaca
     7501 agcgaaagca atgttgactg tattacatca tcatccatca atctttgcat ggtctggtca
     7561 taacgaacca ccttggaatg cgccgtggat gaagtataaa tatccagatt ataagccaca
     7621 gcaaaatcgt tatttaacgg atgctgttgg agctgttttg gcaagtgata aaacacgtta
     7681 cagtcatgat tactcagcaa ccgatgaaca tttatggatg ggatggtatt ctggtaattg
     7741 gaaagagcat gccaaacgta gcaaagtgac gattgtcagt gagtttggcg cacaggcttt
     7801 gcccgatgtt gcacgtttaa aaaagattat tcctgctagt gatttatggc caagaaccct
     7861 agatgccaaa gacccgatgt ggaaaagttg ggattaccat aattttcaag tgcaagaaac
     7921 ctttaaaaat gcgggtatta gtcgtggtga ctcattagaa agttttgtca aaaactctca
     7981 agaatatcaa gcaaagctca ctcaattagc aggtgagtca tatcgcaggc aacgttatca
     8041 acctgttgct gctatgttcc aatttatgtt taatgaaaca tggccttcta ttaattgggg
     8101 cgtcatggat tatacccgtc aagctaaagc agggtattgg gcattaaaac aagcttatca
     8161 gccagttttg gcaagcatag aatgggatgt tgataaacta aaaaccaacg atgaagcatg
     8221 ttttgtgtta tggtcgatta atgatacttg gcaagattat ccatcgttaa aattgcgtta
     8281 tcgcttatta ttagccaata aagtggtaaa agaaggacaa aaagaaatta atttaatggc
     8341 agatgaagcg catcctgagt gggaattaaa atggcgaggt ttgtctcaag ggagttatcg
     8401 tttagaggca acattgagcc agcaaaagaa gataatcagt aataatagtt ttgagtttgt
     8461 tgttgaataa aaactgtttt gcctaatagg atatgttatg gctttttatt ttgaaaaatt
     8521 cgagcatcgt aaaccaccag agccagttcc gcatcgtcca tatatagagc ttgtttggca
     8581 gtttttagcg gtgattgcct tagtcttagg cggaaattat atttattggc gatggacagc
     8641 atctttaaat tatgatgcct tgtggtttgc tattcctcta gcctttgcgg agacgtgtgc
     8701 ttattttggt ctgttcttgt ttgcatttaa tttatggaaa gttaaagatt atcctcaaca
     8761 agaaccgcca aaaacgataa gtgactgttt agctcacgca ctagatgatc atgaagatag
     8821 acctgtcagt gttgatttat ttattgcaac atatagtgaa gaagaagaat tagttagact
     8881 ctctattcaa gatgccaaaa aagttaatta tccacaccat attgattata aaatatttgt
     8941 tttagatgat ggtcgcagag cctctatgaa agctgtttgt gatgaagagg gagtgggtta
     9001 tatcacacgc agtaataata ttggttttaa agcaggaaat ttacgtaacg ccatggaaaa
     9061 aacccatggt gattttattg tgatttgtga tgccgatact cgtgtttttc cgactatttt
     9121 agaggataca ctgggttatt ttcgtgatcc aaaagtggca tgggtacaaa ctccacaatg
     9181 gttttatgat attccagaag gcaaaccatt gcctgatgtg tggcaaaaat acgcagggcg
     9241 tttgggttat tatgcaggac gaggcttaga aaaagtcgtt ggcccgattc aattaggacg
     9301 agaccccttt gtgaatgatc cacaaatgtt ttatgacatt attcaacgac gtcgtaattg
     9361 ggcaaatgct tcgttttgtt gcggtgcagg ttcaattcat cgccgtgaag cggttatgca
     9421 aggcgcatta agagcttatg ctaatgcgat tgaaaaagaa gttcatcatc aaacaaaaga
     9481 tgttgatgat gcagagttac gcgaaacttt gcaagatgct attcgtactc aagctgtgat
     9541 ggataccgaa ttaaccccct ataagtttca tgtttcagaa gatatttata cgtctattgt
     9601 tttacattct gacccagagc atcattggaa atcagtcctg caccccaaag ttgaatcgaa
     9661 aatgttgtct ccacaagatt tgcaaacatg gatgattcaa cgatttaaat atgcgggtgg
     9721 ttctttagat attgcactaa gagataaccc tgtttttcag ggtaatttga cgtggccgca
     9781 acgggttatg tatatgacta cgttttggtc atatcttggt tgtatttgga atgtgatttt
     9841 tttggttgca ccaacgatat ttttaattac aggtatatca cccgtctccg catactctac
     9901 acctttttat tggcactttt tgccatttat tatttgtact gagttagctt ttatgtttgc
     9961 gacatgggga gttaaagctt gggatggtaa agcctcttat ttatcttttt ttcctgtcaa
    10021 ttttcaagca ttagcgaatg tgttgcgtgg tgaaaaaatt aaatttcctg ttactcctaa
    10081 agagcgtcaa gaaggcacat ttattcattt agtctatcca caattggcca ttattgtgtt
    10141 aagtattatt ggtttagtga ttggttttat caaaatggcc ttagatatgc ccttggatgt
    10201 cagtggcttt ttgattaatg ttttttggag cataaacaat attgctgcaa tgtatcctct
    10261 agtcagagcg gcgttgtgga aacctgatta tcaagtagct taaagtggag atgatagatg
    10321 gctttaaagg atgatttaat tacagcgcgc agttatatcg tatttgtgct tggtttatta
    10381 gttgcatttt caatggtgta ttacattgag caaaaagcaa ctgttgcccc ccaagttatt
    10441 ccaccagtca tgatggttaa gagtgttgtt aatctaccta ataatcaagt acaagcgact
    10501 ccagttgtgc aatcagaacg tgctatagat gaacaagtga ttgataaacc tgtgcctgtt
    10561 gtcgccgtac aaaccgtggt tgctgaagaa acagaaaaaa aacttgatgc gccagttgtg
    10621 acagcacaag tatctacaat acaaccacaa caaatggtgg cagataatgc tatgaaacct
    10681 aaagtgccag atgatgttgt gctaaatcct ttgatggtta agaagcgtca acctcgtgtc
    10741 ttgaccgccg aagaaatgga atgggcgcgt attgcatggc agtattttgc caataacacc
    10801 caagcaacag gcttggtcaa ttcagtcaat ggttatccat ccaccaccat gtgggatacc
    10861 gcctcttata tgttgggggc tatttcggct cagcgtttag gcattattag caaaaaagaa
    10921 ctcaatgagc gcatggcgaa tgtcctagtt gctttagaaa aaatcccttt atttgatggc
    10981 aaattaccca ataaatccta taacactaaa accttggcaa tggtcaatta tgctaataaa
    11041 gatgctacta aaggtattgg ttggtcagcg attgatattg gccgtctatt agtgccactc
    11101 aaaattattt atcaaaaatt tccagcacat cgtccgagta ttaataaagt attagcacgt
    11161 tggcaaacac aaaacatgtt agatagaggc cttttatttg gagcgtcatt aaaagccgat
    11221 ggaacagtgc atttattaca agaagggcgt ttaggatacg aacaatacgc cgcaaaaacc
    11281 tttgctttat ttaataattg gagtgcaaat gcagcggcca gttataccaa ctatttagaa
    11341 tatgtgataa ttgatggtat taaaattgcg attgataagc gtgaccccaa acaacatggt
    11401 gcatataact atatgttgag tgaaccattt atgttagatg gtttagaatt tggatttgat
    11461 aaaaactctg cacaattagc cgaaaaaatg tttttggtac aagagcagcg atataagcgt
    11521 acgggtattg ttaccgcagt aacagaagac catatagatg ttgcgccgta ttttgtttat
    11581 aacactgttt atgtggcagg aaaatcatgg gcagcaatta ctgataaagg acaagatgcg
    11641 agcgctttta gagccttatc aacaaaagct gcttttgcat ggtatgtttt gtatcagcat
    11701 aactatagtg acaagttgat taacgcagtt aaaggcttaa atgtggctaa tcaaggatgg
    11761 tatgcaggct tatatgaaga gggcaaacgc cctaataaag tgattactgc taataccaat
    11821 ggtgtgattt tagaaagttt agcttatctt aaatctggca aattattagc taattgaggg
    11881 catcaataaa tgattaaagc taaagcattg acgttgtgtt tagcgatgtt agcaaatgca
    11941 agtgttgtgg tgtctgctca ggcgatgact gtatccgctc aacctccgat tgcctataaa
    12001 cagcgtcaag gtgctttaac tgaaactgaa atggaaatgg ccaaaatagc atggcgttat
    12061 tttcaaaata attatcaacc tgaaactgga ttggtgaatg cagtagatgg ttatccatcc
    12121 accactatgt gggataccgc ttcatatatg gcagccttag tcgcagcgca tcaatttaat
    12181 tttattgata aacaaacctt tgatagtcgt ttaactaagt taattgccac tttaaataat
    12241 ttagtgttat ttcggaatga attgcctaac aaggtatatc acacaaaaac agccgaaaaa
    12301 gtgaattatg ctaataaggc aggtgaaatt ggtttttctg cacttgattt aggcagaatg
    12361 ttaatttggt taagaattct taaagaaaac tatccagaac atgccaacgc aatagacagc
    12421 gttgtcttgc gttgggattt tagcaatgtg gttgataagt gtggcacgat gtatggtgca
    12481 atgcttaatg cagataagaa aaccgtttat atccaagagg gacgtttggg ctatgaggag
    12541 tatgctgcta aaggttttca actgtggcat attcctactt gtttatcaac aaggccagaa
    12601 ccatttgcag taatgcccat gtattgtatt aatgtgcctt atgatacacg agacccacga
    12661 gagttaaatc aacataatta tgtggtttca gagtcatatg tgttggatgg agttgagctt
    12721 ggatgggata ttgtcggcga tatgcacagt gctgataacg tgtttaccga tagaatcatg
    12781 gagaattttg cccatcgtgt gtatcaagta caagaaaatc gttataaaga agcaggtatt
    12841 atcaccgcac gttctgagca tcaactagat aaatcccctt attttgttta tgacaccgtt
    12901 tataccgatg gttatccatg gaataccatt accgatagtg gtaagtatgt acctgagttt
    12961 gccgcagtat cattaaaagc agcgttaggt atgtgggtat tatgggatac gccttatacc
    13021 gatttattgg caaaccatat ttggcatcag tatgttcccg aaaaaggtta ttacgaaggc
    13081 gtatatgaaa atggtaaagg gccaattaaa gcatttacgg ctaataataa cggtatcatg
    13141 ttagagagtt tattatataa agtaaaaggt aaattactca ccatgaataa ggcaaccaaa
    13201 cctgttgttt ctttatggga aaagaccatt gctgatagtt ataacggtga gaataaaaaa
    13261 agaaatcgtc ctcaaatttg tgatatgaca ttaagcgaca taaaaccatt aactgaaaag
    13321 aaaacatggt ttttttggcc tcaaaaatca acaaacgcgc caattcgtga atttaaaccc
    13381 ttaaataatg aatgtaccgt acaaaagacg tgttcagtat gccaagaaaa agcacctgtt
    13441 atattgccag cttggtcatc ctgtcactaa gtctatcttc ttgtggcgtg gttgttagac
    13501 aggctaatca attatatcaa cagattgatg cagggagttt gttgcatcaa ggacgtatcg
    13561 cgcctttaac gagcaaggaa attacgtggg ctaaaactgc atggcgttat tttatcaata
    13621 atactgataa cgaaacgggg ttagttaacg ctttagaccg ttatccgatg gctacaccgt
    13681 ggacaatggc ggatagctta gcggcattat tatcggcaca tcaattaggt ctgattgaaa
    13741 aaatagaatt tgatcagcgg ttaagccgat tgttggcatt tatgtcacaa atggatttgg
    13801 tcgaaggtca attacctaat cgtttttatc aaaccaaaac aggcaaaatg cttaaccagt
    13861 atagcgaagt aggtgaggca ggttggtctg ctttagaaat cagtcgttta ttaatttggc
    13921 taaaaattgt cggtgaatat tatcccgaat accatgaata tttagataaa attgtgttgc
    13981 gttggcattt ctgtcaagtg attgatgatt gtggtcaatt aaagttaaaa accaaagcca
    14041 ataatcaatg gcaatatgag tctgaaggac gattaggtta tcagcagtat gcaggttttg
    14101 gctttagttt atggggattt aatagtgata aagcattaaa ttttaatcct aatgagcgta
    14161 ttaaagttga taatattaat ttgttatacg atgcacgaga cccacgtttg actcatattt
    14221 ctaatccaat cttaacaact ccttataatt tattaggatt ggagtttgca tggcaaattt
    14281 ttccaacaga ggcggctaaa cagcaaaaat tgtttataga acaagcacag atggtttatc
    14341 gagtacaaga aaaacgttgg caaaccgaaa aaatatttac cgcaaaagcg gattattatc
    14401 gactcactga accaagaagt ttaaatgata gtgtatttgc aaatggccaa ccttggaata
    14461 ctattaatga taaagggcag tggtatccta aattggctgt tgtttcaaca aaagccgctt
    14521 tttcaatgtg ggtgttatgg aaaaccgatt acacgcaaca attaatgaaa gtcatagagg
    14581 ctctctatga taaagataga ggatggtatg aagggcgata tgaagctagc ggtgcttatg
    14641 aacgacaaat aaccagttat actaatgcta tggtgttaga gtcattgtta tataaggcta
    14701 aagggcctat ttatcgtcaa gttaatcaaa ctagacattt taatcagcga ttggacaaaa
    14761 tatatgcgcg tccacaacaa tgtttccctc aagagcgacc tgtttgccca ttataaaaat
    14821 aagttgcgag atgtagtttt tatcttggct ttatcactta atatatcgct tagtcatgcc
    14881 gctaccatta gtaataatga ttattatcaa acaggttggg cattttatcg tgcaggacaa
    14941 accgcagaag cagcaaaaac atggcaagct ttagccagta gtagtgaaca acaaagtgat
    15001 gaacaaaaaa aacaagcggc tttgtcggcg gtatttgcta cggtattgtg gcaaaattta
    15061 gaaaacccac aagcttataa tacatggtca gatgcgatta gattatattt ggaagctaac
    15121 agttcttggg agcaagagcg tgtcatgctc aagcaacgta ttgccacagt cagacaagca
    15181 ctgcaacaat taagtggcga tgtagtgcca actattgagc catttatgtc ggttttatta
    15241 gaaatggaca gccgatatgg tttaacagag tttcaaactc caaaatctgg tcttaaatta
    15301 acgaatccag atagtctttt ggagttgaat cagaattttt tagcctcacc acctgtggta
    15361 attaatgaag aaaatgtgtc tttaacaacg acagtagatt ctagctctag cgaaatgcag
    15421 ccaacttcac cgcaaggaga gagtaataat gagcaaacag tgcaaacatt aacgaatcaa
    15481 caacattttg cagtggatac tcagtccgaa acggtgaagc ctacaaatga ccaacaagat
    15541 aaatcagaca aagaacaagc aaataacgaa aacaatactc aaactttgcc tgtagaggca
    15601 gaaatagaac tcactacgcc agaacgaggc catttaatta agccagaaga aaccaatgtt
    15661 aacgaagtca atgttgaagg tactgctttt caacgccatg taacaccaac tcccgaagaa
    15721 taacacatta gtaacttact tgattctgag tacccgatat tcaaggtctg caataaacat
    15781 agctacttgc ttttctaact cggcaaaagt ttcgtcaatc tctgtgaatt gttgtttttt
    15841 acccatttca caaagtttat tagcaatatc ctcaattaat ggccgaccaa aattagccga
    15901 ttcacctttt aatttatggc taattaaata tatttgttct gcatttcgtt gtacaactgc
    15961 ttgtttgagt aattgcatcc gttcagggct ttctttgata aaaacttgtg ctaactctgc
    16021 gacaaaacta ggatcaccac ccatattttc tagagttaat tcataatcaa aacaaacagt
    16081 ggggatagtc tctgtattca tatcaatctt gtttcgatgt tgaggaggat ataacaaaag
    16141 cacacgatca atttcagcct tgagttcatc aaccataatc ggcttgctaa cataaccatc
    16201 catgccttct gttaaaaaac gttctctatc acctgtcatc gcatgggcag tcatggcaat
    16261 aattggaata tgaggatggt ttttttcttc acaccaatga cggatatgat gtgtggcagc
    16321 aataccaccc atcgtgggca tttgtatatc catcaaaatt aagtcaaaac gatcactact
    16381 aatcgcattg actgcttcta aaccatcttt ggctaaaaag cattgataac ctaatttgtc
    16441 taataaacga atagcaagtt tttgattaat agtgttatct tcggctaata aaatatgtag
    16501 tctatctgat atttccatat tgctgatagg ctcattagca gttaaggttc gcacacgctg
    16561 agggcgaggc gattgtgttg ttaatgaatt atttttgtga tcagccgttt gtaatttatg
    16621 tttatcaact gtacctaaaa cgtgcaataa acaattatat aattccgatt gatctattgg
    16681 tttggataaa aagtctgcaa tgcctagatt ttttaaagta ttagagtctg gtggggcagc
    16741 aatagagctg agcatcacta aacgaggtga gccaagcttt ttttcagtga taatatcttt
    16801 agctaaacta aacccatcac gatcaggcat ttgtgcatca agtaaaacta aattaaaagg
    16861 tgttccttct aaggcatgat cgaccatttt catcattgct gagcgcgcat cgcccaccac
    16921 aaaaggatcc atgccaaagt tatgcaacat atcgtacatt aaacggcgat ttgttgaatt
    16981 atcatcaacc acaagtacag gcagtttttt tagcgtatca ggcgttacag gtgaaacttc
    17041 ttgctcatgg cctatggtaa ctggaatgct aaaactaaat tcacttccta cacccatttg
    17101 acttttaacg gttagtttgc cacccattaa ctcaattaac ttggatgaaa tggccaagcc
    17161 caagcctgtt ccaccatatc ggcgtgtaat tgagctatca ccttgagaaa aagattcaaa
    17221 aattttatcg agtaaatgtg gctctatacc aatacctgta tcactcacac agacaaaaag
    17281 ctcattttgt tgattagtat ggcaagtaat agctatcttt aaaccgactt cgcctgtctc
    17341 ggtaaactta atcgcattac ctaataaatt aatcagcact tggcgtaagc gattggcatc
    17401 agtaatcagt gaaacaggta cctgattttg tatgtcgcaa gttagctcta tacttttttc
    17461 ttgagcgcgt aaagctaagg gacgtaatgt gtctctaatc aattttctaa cattaaagtc
    17521 tgtaggatca atcaccattt ttcctgcttc aattttagaa aaatctaaaa catcattaat
    17581 cagccctaat aatgacattg ctgatgaaga aaccatttgt aaatattctt cttgttcagc
    17641 attaagagga gtgtctaaac atagtttagt catgccaata atgccattca tcggggttct
    17701 gatttcgtgg ctaacattcg ctaaaaaatc acttttggct tgattactgg cttcagccgc
    17761 ttctttagct tcaataatgg cagcttcggc tattttacga gcatgaatat taaccattgt
    17821 gccaagacaa cgtctttgag gagaatcttg attttgaact tttccctgaa tttgtaccca
    17881 aatatattcg cctgatgaat ggcgcatttg tacttcaaga tttaaatagt ggctagattt
    17941 ggtgattaat gcactaattt gttgttcaaa tattgaggta tgatgggggt gtaaataatc
    18001 ataccaaagt gcaaaatgat tatttttaga gggattaata tctaataaat caagccattc
    18061 ttgactaaaa taaatagagt ctgagtcaat ttgccaatcc cataaaccaa tttctgcact
    18121 agcaacggct aactgaaaac gctcttcaga taatttgagt acatcactta actcttgata
    18181 agaatccact aatttattta ataataatga aacagaatct agacgttcat ctagaccgtt
    18241 atagtccaca ccaatttttt tggataggcg ttcagccaat tgtttaagat tattaatagt
    18301 ttgttgttgc agtgccaact cattagccaa gcgttggttt gcccagttaa gttcttggga
    18361 gctaatttct aaactacgac tgcgtaattc taaatcccgt gcagattgtt gataagcttc
    18421 atcaacgtgt tgtaagaagc tgctaaagtt atctagcagc ttatgtactg tgtgtgaggt
    18481 ggtatgatgt tttaagtcgc tcaattcttg agtaataaaa tctaggtttg aaacatcttg
    18541 tatatcaaac aaacgacgta gctgcctttt taacagtttg gtctcaatca tgtttcgctc
    18601 aaataggtaa tagtcatcgt ttgattatgt agtttacaat ctgtggtatt actaaaagga
    18661 ctaatttctc cattagaata aaaacctgtg atgatagttt cttcaccaaa aatatcactt
    18721 acagcatcga cttcttcatc aatacgtccg ccaattaaca attttcgtcc tacacaactc
    18781 actaaaatgg ctaatcctgc ttgttgatgt tgacgtaaag agttgactaa aatagcagct
    18841 tgttccgcac catcaattaa ggcatctgta gaggcgtgca ttaattgtaa atagccacca
    18901 tcgattaaat cacccgccaa aatcaaactg ccttctgcct cattaatgcc taaaatggta
    18961 cgaataatgc caacatcatt gccatcagca ctgagcatag aaaaaggaaa gagtaaacct
    19021 gaagcaggca atttatcggc ataatcaccc aaatagcgtt tataaagctc tagtgctggt
    19081 ttgccatcga gttcgtataa aacattgcca atatgtttag taattcgtcg tgttgtgcca
    19141 aaacctttcc agccaccaaa acatgcagag gttaattgga tatgagtccc ataaaaacca
    19201 agtgctaaaa tatcctcagt tgagcggtgt tctccgttta aaataaaggt tcggctaaat
    19261 tggccactgt cacctgctaa accaccgcta atgactgttt ggcttggtaa ttgacttttt
    19321 aaaccttcaa ttaaggcact accattaata ttaacgcctt ggccaaatag caaaatatta
    19381 ttgagtgagt gattttgtag ttgttgtgcc aaacgtagtc cagcgtcaaa actatcatcc
    19441 attgatttga gagaggtttt gactaaaaca aaatcaggat gatcaaaata tagtgccgtg
    19501 agcaccacac tattgtctaa tacaccatct tgataaattt cgcctgctgt tgaacagcca
    19561 attaacaaag cttcattaaa ctcagtgcag agttgtttta taaatgtgtc atgttcaaaa
    19621 tggctaattg aagcaaatac caaaaccagt tgaggtttaa tactctttaa ttcggcaatt
    19681 tggcttgaat taagttcaaa attattggta taaatttgtt taatattcat ttagttttta
    19741 taatccatta gcgacatagg ctcgaggaat aagcaagaga taaacttatt ctgtataaca
    19801 tttgagtcta tattttttgt aaaaagtgtc aagccagagt tatattttta tcaaacttga
    19861 tataaaaacg atttactttg gatagagtct gtttacgttt tgggtgtgag ctgggttgtt
    19921 gcccctaaat atttatatac aaaagcaata gagtgcgctg ataatgacaa ataaatcaat
    19981 ggtgttaatg ttgtcgatat tgttgatggc ttgtcaaaac caagctatta gacaagggcc
    20041 agccccaatt attcctaaag gtcagccatt tgtagaaaaa gactttgatg aaacaatcgc
    20101 aattacaccg ccacaatata ttttcccaat ttctccacga ctatcactca tccctgattt
    20161 tgattttgat ggtgttaatg atgaagtaga tcaatgttta gatacaccat caaatgtcaa
    20221 agtagatcaa aaaggttgta ccattgatcc tgatgacgat aaagacggca ttaaaaactc
    20281 tgtagataaa tgccctaata cacctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20401 nnnnncctat taattaataa tgcaggggtt agccaacgta gtttgattaa agacaccagt
    20461 ttggctgttg atcgtacctt aatggagatg gattattttg cgcctgttgc attaacaaaa
    20521 actattttgc cttatatatt agcacgcaaa cgtggtcgtt tggtggcggt atctagtatt
    20581 gcaggtttag ttgcaacacc aatgcgttca acctatgcag cagcaaaaca cgccatagcc
    20641 gcattttatc aaaatcgccg taaaacctgc ctttaggccg aggatataag gcgaggttga
    20701 taaaaggaag cgcgggagtt tatggctccc gcgtttgcac ctttttatat tatagttgtt
    20761 gtttcactgc gacttattgt tgttatatcg cttatctgac gctttttatt gttattcgtc
    20821 agttctactt gatgactttc aatctttcga ttgctgtcac tatttagagc tagttgcatt
    20881 tgtcatcctg actacaccag cattattatc gttatctatt gttgttatta tcttgcgtac
    20941 aatttgttgt tatctgttac gcaaataata tattgcatag agtgtgccaa cttttttaaa
    21001 tgagtatttt gagtgataaa tatacattat gttagttttt tgagcttttt tgctttttta
    21061 aatgttactt ttaaataaaa ctcaggtaac agtaacatct tgacaacgtc aaagtaacga
    21121 tttgttaaca ataggtaacg caaagtaaca ttttaacatc tgcaaaaaat caacaaaaaa
    21181 ttaactgtcg ctagttacaa agcttgatag ctcaaatatc atctagctcg tataattagt
    21241 ttgtatttaa tgttatttgg gaatatcgcc atgttggcta attgtgataa acaggcatct
    21301 aaggccaaaa taaaaaaaat ctttttacta tcttcaatat gtttactaac tgcttgtggc
    21361 ggtggtggtg gcggagactc gtcaccatca accaatactg ataacaatag caataataat
    21421 tctgagctta atcaagcacc aactaatgta gctattaaaa aagcatcagc aactagctct
    21481 tcagaagtac aaattgaatg ggatagtgcc agtgatgaca aaacgattgc cactgactta
    21541 agctatgaga ttcatctctc tgaaggtggc gattttaccg tgagtgactc aacacgtaaa
    21601 ttttccggta aaaatatttt aaacgctgca attacaggct taaaagcaca aacagcttat
    21661 caagttaaat taatagctat agatagccaa gggcttaaaa ctattagttc tgcaaaaaca
    21721 gtgacgactt taaatgaaag cactccaccg attaatcaag ctccaaccaa tgtatctttg
    21781 acaaaagtaa cagcgctaac aacaaccagt ttgtctattg aatgggcagt ggctaaagat
    21841 gatagcacct tgccagaaaa attagtttat gaagtgcata taactgaggg aaaagcagac
    21901 tttcagccaa gtttaacaac acttaaaaag accttatcaa acacactaag tacacaactt
    21961 gatgaattaa aagtacaaac tgattatagt gttaaattgt tagtaaaaga tgagcaaggt
    22021 ttgtctacta gtagtcaagt gttgtttgtg acaacgctaa aggccgatag tgtcgcagtt
    22081 gacaaaataa atgatacagg cattaccata tgcgctaatg acaacacgat tttcagtcaa
    22141 tgtaatgtga caaatttagg tggctggttt aattggcaac aagatggtca aatgggtaga
    22201 gatgccttgg cggctcaaaa acaattaaag aaaataggga tcggggttgc tggatttgat
    22261 tttagtaagt taggtatcaa aggtgagtta ttatcggata acgcaacaat gtggtcttgt
    22321 gtcaaggata atttaacagg cctgatttgg gaagttaaaa ctaatgataa ggctttgcga
    22381 gatacacaaa atacttatag ttggtataac acagatggca catcaaatgg cggtttcgtt
    22441 ggaaagcaag agtccaataa caccttagat tatacaaata aggtaaatca atcggcatta
    22501 tgtggtttta gtgattggcg tttaccctca cgccaagagt tagagtccat tcttgattat
    22561 ggtcaaacaa ccctagcaac tgtcgataat aatttgtttt taaatatgca gcgtaagtct
    22621 tattggacat ctacaacagt cgccgtgtca cctgcaagtg cttgggcgat aaactttgca
    22681 gatgctagtg atggtcagca tagtaaaagc aatatgttcc ctgttttgtt ggtacgtggt
    22741 gg
//
LOCUS       DAOQMB010000003        21339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_845_length_21339_cov_15.992248, whole genome shotgun sequence.
ACCESSION   DAOQMB010000003 DAOQMB010000000
VERSION     DAOQMB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 21339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21339
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_21339_cov_15.992248"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="PLY05_00310"
     CDS             <1..407
                     /locus_tag="PLY05_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNC01772.1"
                     /translation="GNDTMLSTYIYNYIIYLLASLAMIGVFSLIYTKITPYDELKMIR
                     EGQTAAACSFVGALLGFSLTLASSIMHNDNLVAFAGWASAAMAVQLLVYALVSRIMPE
                     IHNELDNNNVAMGALLGGVSLTFGIINAACLS"
     gene            431..694
                     /locus_tag="PLY05_00315"
     CDS             431..694
                     /locus_tag="PLY05_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01773.1"
                     /translation="MIPAKVLQDFDALPPDLQVQIFDFIEFVKAKHQQKIQNAPQSLD
                     EPPTIGLGDRMANYFKDIPPANCMEEEVFIPHREHYPPVSFDE"
     gene            787..1122
                     /locus_tag="PLY05_00320"
     CDS             787..1122
                     /locus_tag="PLY05_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC01774.1"
                     /translation="MLEAILGSISKERILIYLYSRDKGYATEIAKTFNTSLTPIQKQL
                     EALEQANIIYCEPQGRTLVYALNPRYPFIAELKLLLQKALSFYPAEQQELLIMNRRRP
                     RRQGKPLTL"
     gene            1119..1664
                     /locus_tag="PLY05_00325"
     CDS             1119..1664
                     /locus_tag="PLY05_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01775.1"
                     /translation="MKHIAQMTLTELAAYISSYLQQKNMPVVLVGGACVSIYCHNKYQ
                     TSDLDFVEKYYTKRSDLKAALAELGFEEQNRYFVRPDVQYFLEFPAGPLAIGDSPVRQ
                     LHELKTDLGVLALLTAQDCVRDRLSAFYHWRDKQALQQAIWVAQLNPIDLNFLREWSQ
                     AEAMLDKFEIFLVELQKAQES"
     gene            1667..2704
                     /locus_tag="PLY05_00330"
     CDS             1667..2704
                     /locus_tag="PLY05_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC01776.1"
                     /translation="MGLFSFVAKQFVDVIEWDNQPDGQLMWRFPFTDNEIQYGAQLTV
                     RDGQIAIFVNEGQVADVFTAGLYKLETRTLPVLTNLKNWDKLFASPFKSDVYFVSTRL
                     QLGRKWGTPQPITIRDSDFGMVRLRAFGMYSYKLTDAQKFYTSITGANSTYTAEQLEP
                     QLRNLIVANISTAMASSGVPFLDMAANQGLMADKIKEVLKPLFVNYGLELDNFVVENV
                     SLPEELQKAIDTRISMGMMGDLNKYTQYQTANAIPLAAQNEGGMAGVGASMAAGMAMG
                     QAMAGAMNKAFNPAAVAQSVLGEQTAAAAPASDDPTAKLEKLKGLLDKGLISQADYDT
                     AKADVLKKLMG"
     gene            2701..3087
                     /gene="speD"
                     /locus_tag="PLY05_00335"
     CDS             2701..3087
                     /gene="speD"
                     /locus_tag="PLY05_00335"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:TIGR03330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNC01777.1"
                     /translation="MKPLGQHYLLECDGVEPSLLANPQQLEQILRQAAQQAQAHIVFA
                     HLHHFGEGQGVTGVLVLQESHITIHTWPEYAYAALDVFMCGQAQPQLAVAYLIEQLQP
                     QQHRWQMIERQSFALSSLAPLGRGQG"
     gene            3163..4620
                     /locus_tag="PLY05_00340"
     CDS             3163..4620
                     /locus_tag="PLY05_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNC01778.1"
                     /translation="MFKAFCPSCGADVPFKSPASVMAVCDYCQTTVLKDADSVKDIGK
                     MASILEDYSPLQIGTTGIYQNQAFTVLGRIQLKYDDGLWNEWYTFFDNGKEGWLSEAS
                     GQYVFTFADGVANDAPAFESLRPLSKYRLYSATDVRTARCTGGQGELPFKVGKGWKTQ
                     TADFRIKDNFLTLDYSDGVPPQRYVGKAVELDQLQCQLLRDQDAIHESAGRIKGQMQA
                     LDCPSCGTQIPYANQEFKHIVCPACSANVELTESKAIVVEKHQQQSYKQTNLKLGDLG
                     NIDGVQYTIIGLLQAAEMDDYSTMPWTEYLLYNTDKGFLWLVESSDGWEKVKVLNEMP
                     DNSRDNLRYQGKTWKKLWTYRAKVGYAAGAFNWRVKVGDITEITDYQANEQKISAEKT
                     AQEVTWSLAQKVPKALVEKWFNKTLFNPSALNRQNQHDNIMREMGFKYDKQQLSYFFI
                     AILWLINMPLIAAGSGSFVLTGIATIVLWILALGT"
     gene            4624..4791
                     /locus_tag="PLY05_00345"
     CDS             4624..4791
                     /locus_tag="PLY05_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01779.1"
                     /translation="MSNIQYLLWATVIVFITTAINLSTNTGSYSSSSSSRSWSSGSSS
                     GGWSYGGGGHK"
     gene            complement(4793..>5272)
                     /locus_tag="PLY05_00350"
     CDS             complement(4793..>5272)
                     /locus_tag="PLY05_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC01780.1"
                     /translation="KKYNSITLEADAHHLMTDVWTSIGVLIAIVAVALTGWYVLDALI
                     AIGVALNILWTGWSLLYRSVAGLMDAALPHAEVQQIKQILDNYSHEHGIIYQNLKTRQ
                     AGARKFISVHILVNGNWSVQQGHDLVDIIETDIEQQIQGCHVLTHLEPIEAPSKHHF"
     assembly_gap    5273..5372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5614..8127
                     /locus_tag="PLY05_00355"
     CDS             5614..8127
                     /locus_tag="PLY05_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNC01781.1"
                     /translation="MLRINLHDAYKTLCLLLNMMLSICAWSAQEIVLTDKTESLSISK
                     QIYYFADNSLEYQLADILRDEQRNKNIWQTANGFIPNYGFTQAAYWVKIIINNKSQKK
                     DWIVYFEYPLMDHIELYVPENGHYKTYISGDSLPVSKRYIKDRGFAFPITLDKQNSQV
                     LYMKLQSRDSMIIALDVMTHDAFRAEQQNVGFWFGMYYGAVLIILLYNLYLYFVLSDK
                     NYFYYVVVLFCYVLIELSLNGMGSVYFWGEMPELAKRIRPLAICLFSFACLVLTVSFL
                     DIKSVKFLNLNIISIFVAVIFIGLIGSFIFPFTLAIQVAMIIAFVAAPVMYATGVYVW
                     QQGNRIGKYFTIGWSGVVVGGMVNVLRAFDILPVNFWTTYGAQLGSITTLLILNTALS
                     DRVRLLQQQSEKSQQDKILEQEEINRLLDRMVKVRTQELLLKTEEAERAKQQAESAAR
                     AKSEFLANMSHEVRTPMNGMIGMTQLLADTSLNYEQKHYINTIQASCEALLRIINDIL
                     DYSKIEAGKLAIENTNFNLHQLLTECASLFAVSAKERELPIYTQIASDVPQWMVGDPT
                     RIRQVIINMLGNAYKFTERGAVTLRAKLREDKGKEGVYILFEVEDTGIGITSEQRERL
                     FQSFSQADSSVTRKYGGTGLGLAISRSLVRLMNGDIGVKSIPNQGSTFWFYVQLYHGI
                     QPIKKHHIEPNQTPFVYPNLSGLHVLIAEDNPTNQMVIVGMLKKYQIEPIVAVDGQDA
                     LSKVMAKPEYFDVIFMDCEMPKMDGYTATKEIKAWINQHRFNDVLIFGVSAHALAEYQ
                     QQGLASGMNAFIVKPLKQQDLLQALTQVMAHKMKQKLAR"
     gene            complement(8124..8669)
                     /locus_tag="PLY05_00360"
     CDS             complement(8124..8669)
                     /locus_tag="PLY05_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNC01782.1"
                     /translation="MLARLLIGMTTLLFSHFCYAEAYGALVSADDKQPTNSPAHNNLV
                     IDAKTLLDAHLPYRKELGLLPYVWSDELAKSAQTWADHLAANNEFKHSDVEYGENLWR
                     GTTGAYSLKDMVDAWGAEKKDFVYGLFPQVTKGGMVGHYTQIVWRYSYQVGCALAQGN
                     GKTYLACHYDPAGNYEGEAPY"
     gene            complement(8731..>9747)
                     /locus_tag="PLY05_00365"
     CDS             complement(8731..>9747)
                     /locus_tag="PLY05_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01783.1"
                     /translation="ISMHPTASALLAAHRAHLLNQLSGENLNITLSREINAYLDWSQT
                     RPINALVKLEDIQRVAQQYVLEQAPTPELQAQILHLVKAAINSPINDKTYIHQLIEHK
                     DYLAIVDKIASHEKLRADIIHTIVGNPVYAKLLSDIVYQALSDYLVNENPLAKKVPGV
                     SSLMKMGKGLMESTGGNAAIESALKSYLHKNTQKIVDISEKILIKTLDAKQIHHVADQ
                     LWHKIKGLSLSTVKQYLKPQDIEFGVETGVMLWNHARHTSYAKEMLNELIAAWYNDFA
                     QQDGVSLLASLNISRESLVQDILVFAQSVVADMLATGFIAERIDAQLNEFYNSEAVAQ
                     IFAS"
     assembly_gap    9748..9847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9848..10617)
                     /locus_tag="PLY05_00370"
     CDS             complement(<9848..10617)
                     /locus_tag="PLY05_00370"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC01784.1"
                     /translation="MLKKLILASLLAISYQASTFAAPALVTDPVALTQSILQQERKWA
                     GLESKTIKVEDFTFSYSEGGSKTAPTVLLIHGFSGSRDNWNRIARQLTKQYHVVAVDL
                     PGHGDTIAPATFEYTLANLAEAVRKFVTTLGIQKDLHVAGHSLGGGIAVLYSALYFLE
                     VRSVMLVDTAGVYQNAGNSLITKPQELTKLLARKEGDFEKLVTQIATYSPPFVPKEIM
                     EGYEKIHMRHLPEQEKLMAGLTEQMKWYTPDTFHTGLKT"
     gene            complement(10745..11431)
                     /locus_tag="PLY05_00375"
     CDS             complement(10745..11431)
                     /locus_tag="PLY05_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01785.1"
                     /translation="MFKKLSVVLLSILCYQQAQADVAVGVGLSTLGVGVQGTFGVNKY
                     INLRLTASAASVDEDFEESGTNYKGTVDFSSYGLITDIHPFGGSFYLSAGYLAHDNNI
                     DLKATCPQSCDIDGQSYQSFPDGQINAGIDFGSGAPYAGIGWGNAMQGGRIYAKFDIG
                     VLFQDKPTVDLNVTGSFRNTNTDIIVNTSDPVFQQEVANEEKALQKEINDADYSELYP
                     VIGLSIGYRF"
     gene            11513..>12624
                     /locus_tag="PLY05_00380"
     CDS             11513..>12624
                     /locus_tag="PLY05_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HNC01786.1"
                     /translation="MKKTNFSFRIDTRWCLDELLKDGYINQRDANLVATTPRPRDKLH
                     WHPLEVIAQFEFINLKKPSHKLNLDTLSAWLADKAEQPLFHIDPLKINAGTVTAVMSY
                     AFAERHGILAVQVDKEFVTIASAQPFHNEWEENLQHTLKNKQIKRVVVNPAHLHRFSI
                     EFYKLAKAIEGANNSDAVASNTNKIGNFEQLLELGNTTNPDANDQHIVSIVDWLLQYA
                     FDQRASDIHLEPRRDTGNVRFRIDGVLHNIYEFPALIMNAVISRIKILGRMNVAEKRK
                     PQDGRLKTRSPKGQEIELRLSTLPTAFGEKMVMRIFDPDVLVRGFKELGLVGDDYDKW
                     QKMVQEPNGIILVTGPTGSGKTTTLYSTLKQLATDEV"
     assembly_gap    12625..12724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13077..13571
                     /locus_tag="PLY05_00385"
     CDS             13077..13571
                     /locus_tag="PLY05_00385"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNC01787.1"
                     /translation="MTTATPDLFHWSDDFNVDIQEVDEQHKVLVDLLNQLHHAIVERR
                     GKTASQEILNQLADYTRTHFLLEESLMRVLHYPGLEIHKQQHEDLIQQIHELQRKIDE
                     EGATVTFELLHFLKMWLIQHINESDKRFGDFFAKSSHLQQYATWNKEVEQTMKKKKWW
                     WKFW"
     gene            complement(13572..14060)
                     /locus_tag="PLY05_00390"
     CDS             complement(13572..14060)
                     /locus_tag="PLY05_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01788.1"
                     /translation="MPFWCRLVLILICLYHTPLFAWQKTYQQSYLLGDNDSRLSAKRL
                     LIQQLQSQAAQEAGTYIQAQSTLINDQLEEKITATATALVKVNILQEQFTTETNGQQR
                     LFLELDAHIDEQEMLQLLNKQQELQQLSQKLEQLEQKLNQLPTAPVVYPSPNAQKSFW
                     GE"
     assembly_gap    14077..14176
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14267..14539)
                     /locus_tag="PLY05_00395"
     CDS             complement(14267..14539)
                     /locus_tag="PLY05_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HNC01789.1"
                     /translation="MTRMVMCRKYQQEMEGLAFPPFNGAKGQTIFETVSKQAWQEWLK
                     HQTMLINENHLNVMQAETHVFLDEQREKFFSNADYERPKGWTPEKK"
     gene            complement(14542..15930)
                     /gene="argH"
                     /locus_tag="PLY05_00400"
     CDS             complement(14542..15930)
                     /gene="argH"
                     /locus_tag="PLY05_00400"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNC01790.1"
                     /translation="MTTPSSSMWGGRFSEATDAFVAEFTASVNFDKRLYKHDIQGSIA
                     HATMLAKVGVLTDSERDAIINGLTAIRQEIEAGQFDWRVDLEDVHMNIESRLTDRIGI
                     AGKKLHTGRSRNDQVATDIRLYLRDEIDQTRAILRRLQTGLLDLAEREASTIMAGFTH
                     LQTAQPVTFGHHLLAWFEMLVRDDERLADCRKRVNRMPLGSAALAGTTYPIDRAYTAE
                     LLGFEAVCENSLDAVSDRDFAIEFCAAASLTMMHLSRMSEELILWNSQQFKFVDIPDR
                     FCTGSSIMPQKKNPDVPELVRGKTGRVNGHLISLLTLMKGQPLAYNKDNQEDKEPLFD
                     ALDTLQASLRAFADMVPALKPNREAMREAALRGFATATDLADYLVKRGTAFRDAHEIV
                     GKAVAYGVQTSKDLSAMTLAELQQFSTTIEQDVFEVLSLEGSVNARNHIGGTAPNQVV
                     AAVQRARSRIGA"
     gene            15987..17051
                     /locus_tag="PLY05_00405"
     CDS             15987..17051
                     /locus_tag="PLY05_00405"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC01791.1"
                     /translation="MQQTANNPQNQTVDDFFLPDLCNMKAVLRLLVVTELLAVFLTLA
                     DLERLTPFPWADLGLTSFLLAWVGLCTATLLCALRKQLSRLKHSYAAFIVLFLFLMMV
                     AFFTFAAEGLLLYLQIRHVGEVDVGTALVRNVLMGGIIGGLVLRYFYLQELLLRRQRA
                     ELTARVQALQARIRPHFLFNSMNIIASLIMVDPDKAEQVVEDLSSLFRASLKAEGEVP
                     LKDEIDLCNRYLNIEKLRLGKRLEFEWRLDGLIPSLKIPALTLQPLLENAIYHGVELD
                     DEGGKLTILVEWTGELVNIVITNPYHQHKKSKHKGNNMATANIKERLKVYYGEQATFK
                     SVSAGNLFTTQISYPLPQQQ"
     assembly_gap    17140..17239
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17240..17587
                     /locus_tag="PLY05_00410"
     CDS             <17240..17587
                     /locus_tag="PLY05_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNC01792.1"
                     /translation="SPRPEARKHITARTHKGMELIPISEIYYFLADQKYVTVRHTRGE
                     ILIGETLKDLEEEFGHHFLRIHRNALLALSYLDGLETNPTGQTFVRFRGIPEKLLVSR
                     RHLPLLREKMQSL"
     gene            17875..18981
                     /locus_tag="PLY05_00415"
     CDS             17875..18981
                     /locus_tag="PLY05_00415"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNC01793.1"
                     /translation="MNGWRKIIISSLWLTAAALGLSGCANLHNVKTEEPEKYVLGENA
                     SEARYFYTRSGLRLFGQWWVPTERPRAIILLAHGTVLHSGFYSPWANELTKHGYAVFG
                     IDLRGWGQSQGYGRRGNVTNYDDYVEDLTLARREIRRRFPNVPVYLQGESLGGAVVLL
                     AHIMDKVTTDGIILNAPAVRINPAAQWSLWGNAQLTKHMPETPLLPLFKSLTGLVLTD
                     PQAKDRFWTDPLTTRTALGGGFVTALQDATERLQLNVNNVRAPMLIIHGTKDYVIPKS
                     SSEFLLKTAQSNDKTLRIIEGLHHSTLHDKQKRQVWDDTIAWLDQHADQALAARKKLP
                     DETEISALEDQVPTQEKAAYRPKYPKLTMTTTRN"
     gene            18985..19296
                     /locus_tag="PLY05_00420"
     CDS             18985..19296
                     /locus_tag="PLY05_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNC01794.1"
                     /translation="MKHLLWPVLVATLLSGCATAVSPVNGSLYTNLRAPMLVTESPDK
                     PTKVGRATVRSILGIYAVGDASIEAAAKNAGITRIHHVDYQSQNILGVMSDFTVIVYG
                     N"
     gene            19393..20310
                     /gene="hemC"
                     /locus_tag="PLY05_00425"
     CDS             19393..20310
                     /gene="hemC"
                     /locus_tag="PLY05_00425"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNC01795.1"
                     /translation="MRTLRIATRKSPLALWQAEYVKASLLKHHPQLTIELVTFTTQGD
                     KILDTPLAKIGGKGLFVKELEQAMLAGDADLAVHSMKDVPMECPEGLAITTICEREDP
                     TDAFVSNRFASLQELPLGAIVGTSSLRRQCQLRALRPDLEIRDLRGNVGTRLGRLDNG
                     EYDAIILASAGLKRLGLQQRIAQSLTQLLPAVGQGAVGIEARSNDTELLALLAPLHHL
                     PTALCVQAERAMNRRLQGGCQVPIAGFATLDNQQLTLNALVGSLDGQSVLRYQAQTTD
                     LSTIEQLGENVADGLLAQGADKVLAAVYG"
     gene            20303..21073
                     /locus_tag="PLY05_00430"
     CDS             20303..21073
                     /locus_tag="PLY05_00430"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNC01796.1"
                     /translation="MANLAGLNIINTRPVNRAQPLTDALMQKGALVTAIPLVENIPLA
                     LSAEHRQYLLNLDLYDVVFVVSPTAAKLGLALLSDYWPQWPIAVQWLAIGEATAEVLH
                     QYGLQAIVPTQETSEGLLQLPCLQQLQQGQKMLVLRGQGGRNLVRDNLQQQGVKVDYL
                     DLYQRQVPINSQQLWQQQKKSPDLVVITSGEILQSWQQLVGEHFSKIPMVVISKRLVA
                     MAKALGVMQVITAASTRPEDIVHAIQQWHKGQTHDISE"
     gene            21104..>21339
                     /locus_tag="PLY05_00435"
     CDS             21104..>21339
                     /locus_tag="PLY05_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01797.1"
                     /translation="MSQTRQPIHVFPKITLKFGFWGRVTVIIVILTVIASILLYYHGM
                     RYKNRELLHYQEITQQLQSLKEQQVHYQSKSILL"
BASE COUNT         6408 a         3730 c         4322 g         6379 t
ORIGIN      
        1 aaggaaacga cacgatgtta tcaacgtata tttataacta tattatctat ttattagcca
       61 gtttggcgat gattggtgtg ttttcgctga tttacaccaa aattacccct tatgatgaat
      121 taaaaatgat tcgtgaaggc caaacggcag cggcttgtag ttttgtgggc gcattattgg
      181 gtttttcttt gactttagct tcaagcatta tgcacaacga taatttagta gcgtttgcag
      241 gctgggcaag tgcagcaatg gcggtgcaat tgttggtgta tgcgttggtg tctcgaatta
      301 tgcccgaaat ccataacgaa ttagataaca ataatgtggc gatgggcgca ttattaggcg
      361 gtgtatcttt aacttttggg attattaatg cagcgtgttt gtcttaggtt gaaataatgt
      421 ggagattaat atgattcctg ctaaagttct tcaagatttt gatgccttgc cgccagattt
      481 gcaagttcag atatttgact ttattgaatt tgttaaagcg aagcatcagc aaaaaataca
      541 aaacgcacct cagtcattag atgagccacc cacgataggc ttgggggata gaatggctaa
      601 ttattttaaa gatatacctc cagctaattg tatggaggag gaggtgttta ttcctcatag
      661 agagcattat ccacctgtga gttttgacga atgattttgc taaagtaact agagaatagt
      721 ttttataatt tactttttag tttaaataaa ctataaagta gtttatatgt aattaggaat
      781 gcagctatgt tagaggctat tttaggcagc attagcaaag agcgaatatt gatttatttg
      841 tatagtcgag ataaaggcta cgccaccgaa attgctaaaa catttaacac cagtttaacg
      901 ccaatacaaa aacagctcga agccctagag caagcaaata ttatttattg cgaaccacaa
      961 ggtcgtactt tagtttacgc gcttaatcca cgttatcctt ttattgcaga actaaaatta
     1021 ttgctacaaa aagcacttag cttttatcct gccgaacaac aagaattatt aataatgaat
     1081 cgccgcagac cgcgtcggca aggcaagcca ttaaccttat gaaacatatc gcacaaatga
     1141 ccttaacaga attagcggct tatatttcga gttatttaca acaaaaaaat atgcctgttg
     1201 tattagtggg tggtgcatgt gtctctattt attgtcacaa taaatatcaa accagtgatt
     1261 tagattttgt tgaaaaatat tacactaagc gtagtgacct taaagcagca ttagcagagt
     1321 tgggatttga agaacaaaat cgttattttg ttcgccctga tgtgcagtat tttttagaat
     1381 ttccagcagg gcctttagcg attggtgata gtcctgttcg gcagctacac gagcttaaaa
     1441 cggatttagg tgttttagca ttattaacag cgcaagattg tgttcgtgat cgtttatctg
     1501 ctttttatca ttggcgagat aagcaagctc tgcaacaagc gatttgggtg gcacaattaa
     1561 accctattga tttgaatttt cttagggaat ggtcacaagc cgaagccatg ttagataagt
     1621 ttgaaatatt tttagtcgag ttacaaaaag cacaggagtc ttaaaaatgg gtttattttc
     1681 atttgttgcc aagcaatttg ttgatgtcat tgaatgggac aatcaaccag atggccaatt
     1741 aatgtggcgt tttccgttta ctgacaacga aattcaatat ggcgcacaac tgacggtgcg
     1801 tgatggtcaa atagcaattt ttgtcaatga aggccaagtc gctgacgtat ttaccgcagg
     1861 cttatacaaa ctcgaaaccc gtaccttacc tgtattaacc aacttaaaaa attgggataa
     1921 gttgtttgcc tcgccgttta aatcggatgt ttattttgtg agcacccgtt tacagttagg
     1981 ccgcaaatgg ggtactcctc aacccattac cattcgtgat agcgattttg gcatggtacg
     2041 tttgcgtgct tttggtatgt attcttacaa actcactgat gctcaaaagt tttataccag
     2101 tattacaggc gcaaacagca cctataccgc tgaacaatta gaacctcaat tgcgtaacct
     2161 gattgttgcc aatatcagca cggcaatggc cagttcgggt gtgccatttt tggatatggc
     2221 cgccaaccaa ggtttaatgg cggacaaaat taaagaggtg ttaaaaccgt tatttgttaa
     2281 ttatggttta gagctagaca attttgttgt ggaaaatgtg tctttacccg aagaattgca
     2341 aaaagccata gatacccgca tttcgatggg tatgatgggc gatttaaaca aatacaccca
     2401 atatcaaacc gccaatgcga ttcctttagc cgcacaaaat gaaggtggaa tggcaggtgt
     2461 aggcgcgagt atggccgcag gcatggcaat gggacaagcg atggcagggg caatgaataa
     2521 agcctttaat cctgctgcgg tggcacaatc agtattgggt gaacaaacag ccgctgctgc
     2581 accagccagt gatgatccaa ccgctaaatt agaaaaatta aaaggtttat tagataaagg
     2641 cttaatcagt caagcagatt atgatactgc taaagccgat gtgttgaaga agttgatggg
     2701 atgaaacccc taggacaaca ctatttactg gagtgtgatg gggtagagcc aagcttgttg
     2761 gcgaatcctc agcaactgga acagatattg cgccaagccg cacagcaggc acaggcgcat
     2821 attgtctttg ctcatttgca tcactttggc gaggggcaag gtgtaacggg tgtgttggtg
     2881 ttgcaagagt cgcatatcac cattcatacg tggccagaat atgcctatgc agccttagat
     2941 gtgtttatgt gtggtcaagc ccagccacaa ttagccgttg cctatttaat tgagcaatta
     3001 caacctcagc aacatcgttg gcaaatgatt gagcgacaat ctttcgcatt aagctccctc
     3061 gcccctttgg ggagagggca ggggtgaggg gtataagcaa ccccacccta accctcccct
     3121 aaattagggg agggaatatt ttaaaaggaa acccgaccgc ttatgtttaa agctttttgt
     3181 cccagttgtg gtgcggatgt accgtttaaa tcacctgcat ccgtgatggc ggtgtgtgac
     3241 tattgccaaa ccacggtgtt aaaagatgcc gatagtgtca aagatattgg caaaatggcc
     3301 agtattttgg aggactatag cccattacaa attggtacaa caggtattta ccaaaaccaa
     3361 gcctttacgg tattgggacg gattcagctc aaatatgacg atggtttgtg gaacgaatgg
     3421 tacacctttt ttgataatgg caaagaaggt tggctgtccg aagcctctgg ccagtatgtg
     3481 tttacctttg ccgatggtgt ggcaaatgat gcaccagcat ttgaaagttt acgaccatta
     3541 agcaaatatc gcctgtattc agccactgat gtgcgtactg cccgttgtac aggtgggcaa
     3601 ggcgaattac cgtttaaagt cggtaaaggt tggaaaaccc aaaccgccga ttttagaatt
     3661 aaagataatt ttttaaccct tgattattct gatggtgttc caccgcaacg ctatgtgggt
     3721 aaagccgttg aattagatca attacaatgt caattattgc gtgaccaaga cgcgattcat
     3781 gaaagtgcag gccgcattaa aggccaaatg caggcgttgg actgcccaag ctgtggcacg
     3841 caaattcctt atgccaatca agaatttaaa catatcgttt gtcctgcctg tagtgccaat
     3901 gtcgaattaa ccgaaagcaa agcgattgtg gtcgaaaagc accaacagca aagttataaa
     3961 caaacgaatt taaagttagg tgatcttggc aacatagatg gcgtgcaata taccattatt
     4021 gggttattgc aagccgctga aatggacgat tactcaacca tgccgtggac ggagtatctg
     4081 ttatacaaca cagataaagg ctttttatgg ttagtagaaa gcagtgatgg ttgggaaaag
     4141 gttaaagttc tcaacgaaat gcctgataac agtcgagata atttgcgtta tcaaggtaaa
     4201 acatggaaaa agctgtggac atatcgcgcc aaagtgggct atgccgcagg ggcatttaat
     4261 tggcgagtca aagtgggtga tatcaccgaa attaccgact accaagccaa tgagcaaaaa
     4321 atcagtgccg aaaagactgc ccaagaagta acttggagct tggcgcaaaa agtgcctaaa
     4381 gctttggtcg aaaaatggtt taataaaaca ctattcaatc catctgcgct taatagacaa
     4441 aatcagcatg acaatattat gcgtgagatg ggctttaaat atgataaaca acaattaagc
     4501 tattttttta tagccatttt atggttgatt aatatgccat taatagcagc aggttcaggc
     4561 agttttgtat tgacaggcat agcaactatt gtgctatgga ttttagcgtt agggacataa
     4621 acgatgtcaa atattcagta tctgttatgg gcaacggtga ttgtgtttat tacaacggca
     4681 atcaatttat cgactaatac agggagctat agctcttcta gctccagccg tagctggagt
     4741 agtggcagca gtagtggtgg atggtcttat ggtggtggcg ggcataaata aactaaaaat
     4801 gatgcttgga cggtgcttca ataggttcta aatgggttaa cacatgacag ccttggattt
     4861 gttgttcaat atcagtttca ataatatcta ctaaatcatg gccttgttgc accgaccaat
     4921 tgccattcac taagatgtgt acagaaatga atttacgtgc acctgcttgg cgggttttta
     4981 aattttgata aataattcca tgttcatgac tgtaattatc taagatttgc ttaatttgtt
     5041 ggacttctgc atgaggcaag gctgcatcca ttaagcctgc aactgaacga tacaacaaac
     5101 tccaacctgt ccataaaata tttaatgcaa caccaattgc aatcaaggca tctaaaacat
     5161 accaacccgt caaagcaaca gcaacaatcg caatcaatac accaattgat gtccacacgt
     5221 cagtcattaa atgatgtgca tcggcttcta gggtaataga gttatatttt ttnnnnnnnn
     5281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntgagacat gtggtgaata tattatgtac
     5401 aataagttta aattgggctt aactatacaa tagcttatgc cttaaaggcc atgtttttag
     5461 ccactaatag actactttag gacttacacg gttatcgttt atttgtaagc attcaacggt
     5521 tttatgctta caaaacatgc ttgtttcgac tttgctcaac acaagctttc gatgaaatgc
     5581 ctaagtccta tatttaataa gaagaattga tcaatgctgc gtatcaattt gcacgatgcc
     5641 tataaaactt tatgtttact gctcaatatg atgttgagta tatgtgcttg gtctgcgcaa
     5701 gaaattgttc tcacagataa aacagagagt cttagcatta gtaagcaaat ttattatttt
     5761 gctgataata gtcttgagta tcaattggct gatattttgc gagatgagca aagaaataaa
     5821 aatatttggc aaacagctaa tggttttatt cctaattatg gttttactca agcggcttat
     5881 tgggtaaaaa ttattataaa taataaaagt caaaaaaaag attggattgt ttattttgaa
     5941 tatccactga tggatcatat tgagttatat gtgccagaaa atggtcatta taaaacctat
     6001 atttcgggag atagtttacc agtttctaaa agatatatta aagatcgagg ttttgccttt
     6061 cctattaccc ttgataagca aaatagccaa gtattatata tgaagttgca aagtcgcgat
     6121 tcgatgatta ttgcgcttga tgtgatgact catgatgcct ttcgtgccga gcaacaaaat
     6181 gtgggttttt ggtttggtat gtattatggg gcagtattaa ttattctttt atataaccta
     6241 tatttatatt ttgttttaag tgataaaaat tatttttatt atgttgttgt cttattttgt
     6301 tatgtattaa ttgagctgag tttaaatggc atgggttcag tttatttttg gggagaaatg
     6361 ccagaattag ccaaacgcat taggccatta gctatttgct tattttcttt tgcctgtttg
     6421 gtattaacag tttctttttt agatataaaa tctgtaaaat ttttaaattt aaatattatt
     6481 tctatttttg ttgctgtaat ttttatagga ctgattggct cctttatttt tccttttacc
     6541 cttgctattc aagttgccat gattattgcc tttgttgcgg ctcctgtgat gtatgcaaca
     6601 ggtgtatatg tatggcaaca aggaaatagg attggtaagt attttacgat tggttggagt
     6661 ggggtagttg ttggcggcat ggttaatgtg ttgcgggcat ttgatatttt acctgtcaat
     6721 ttttggacaa cctatggcgc gcaattgggg tctataacta cgttattaat tttaaatacc
     6781 gcattaagtg atagagtgcg tttgttgcag cagcaaagcg aaaagtcaca gcaagacaaa
     6841 attttagaac aagaagaaat taatcgctta ttagatcgta tggttaaagt tcgtacccaa
     6901 gaattattat taaaaacaga agaagctgaa cgagcaaaac aacaagctga aagtgctgca
     6961 agagctaaaa gcgaattttt agctaatatg agtcatgaag tacgtacccc catgaatggt
     7021 atgataggca tgacacaatt gttggcagat acctctttaa attatgagca aaaacattat
     7081 atcaatacca ttcaagcgtc ttgtgaagcg ttattaagaa ttattaatga tattttagat
     7141 tattcaaaaa ttgaggctgg taaattagcc atcgaaaata cgaattttaa tcttcatcag
     7201 ctactgacag agtgtgcctc gttatttgct gtatctgcca aagagcgcga attacccatt
     7261 tatacccaaa ttgcaagcga tgtccctcaa tggatggtgg gtgatccaac acgtattcgt
     7321 caagttatca ttaatatgtt ggggaatgct tataagttta ccgaaagagg tgctgtaact
     7381 ttacgcgcca aattgcgtga agataaaggt aaagaagggg tatatatttt atttgaggta
     7441 gaggatacag gcattggtat tactagcgag caacgtgagc gtttatttca atcgttttcc
     7501 caagccgact catctgttac tcgcaaatat ggtggaacgg gtttgggatt agccattagt
     7561 cgttcattgg ttcgtttgat gaatggtgat attggtgtca aaagtattcc caatcaagga
     7621 tcaacgtttt ggttttatgt acagttatat catggtattc aacccatcaa aaagcatcat
     7681 atagaaccta accaaacgcc atttgtttat ccaaatttgt caggtttgca tgtattgatt
     7741 gcagaagata atccaactaa tcaaatggtg attgtgggta tgctcaaaaa atatcagatt
     7801 gagccaatag ttgctgttga tggccaagat gctttgagca aagtgatggc caaacctgaa
     7861 tactttgatg tgatttttat ggattgtgaa atgcccaaaa tggatggtta tacagcaacc
     7921 aaagaaatta aagcatggat caatcagcat cgttttaatg atgtcttgat ttttggggta
     7981 agcgcacacg ccttagcaga atatcaacaa cagggtttgg catcaggcat gaacgccttt
     8041 attgttaaac cactcaaaca gcaggattta ttacaagcat tgacacaagt gatggcgcat
     8101 aaaatgaagc agaagttggc acgttaataa ggtgcttcac cctcgtaatt gccagcagga
     8161 tcataatgac aagctaaata tgttttgcca ttgccttgcg ctaaggcaca acctacttgg
     8221 tagctataac gccaaacaat ttgcgtataa tggccaacca ttccaccttt agtgacttgt
     8281 ggaaatagac cataaacaaa atctttcttt tccgcacccc aagcatcaac catatctttt
     8341 aaggagtacg cgcctgttgt gccgcgccat aaattctcgc catattcaac atcagagtgt
     8401 ttaaattcat tgtttgcagc taaatgatcc gcccatgttt gtgcgctttt tgctaattcg
     8461 tcagaccaaa cataaggcaa taaacctaat tctttacgat atggtaagtg cgcatctaat
     8521 aaggttttgg catcaataac aagattatta tgagcaggac tattggtcgg ctgtttatca
     8581 tctgcagaaa ctaaagctcc ataagcttct gcataacaaa aatgactaaa taaaagggtt
     8641 gtcatgccaa ttagtaaacg tgcaagcata aaatatcctt gatgatgctt gccgtttagg
     8701 ctgtgcgatc aagcacagcc taaagcaagc ttaactagca aaaatttgag cgactgcttc
     8761 tgagttatag aactcattta attgtgcgtc aatacgctct gcaataaaac ctgtggcaag
     8821 catatcagct acaacagatt gggcaaagac taaaatatct tgcaccaaac tctcacggct
     8881 aatattgagg ctcgctaata agctgacacc atcttgctga gcaaaatcat tataccaagc
     8941 cgcaattaat tcattgagca tttctttggc atagctagta tgacgtgcgt gattccataa
     9001 catcacacct gtttcaacac caaactcaat atcttgcggt ttaagatatt gtttcactgt
     9061 tgataaagac aaccctttga ttttatgcca aagttggtcg gcaacatgat gaatttgttt
     9121 ggcgtctaaa gtcttgatta atatcttttc gctaatatca acgatttttt gggtgttttt
     9181 atgtaggtag ctttttaacg cagattcaat cgcggcatta ccgcctgtgc tttccattaa
     9241 acctttaccc attttcatta atgaagaaac acccggtact tttttggcta aaggattttc
     9301 gttaaccaaa tagtcactta aagcttgata aacaatatca gataacaatt tggcataaac
     9361 aggattgcca acaatagtat gaataatatc agcacgtaat ttttcgtgac tggcaatttt
     9421 atcaacaatg gctaaataat ctttatgctc aatgagttga tgaatatagg ttttgtcatt
     9481 aatagggctg ttaattgcgg cttttaccaa atgcaaaatt tgtgcttgta actcgggagt
     9541 tggtgcttgt tctaaaacat attgttgggc aactcgttga atatcctcta gtttaaccaa
     9601 agcattaatt ggacgagttt gtgaccaatc caaataggca ttaatttcac ggctaagcgt
     9661 gatattaaga ttttcgccag ataattggtt taacaaatga gcgcgatgag cagctaataa
     9721 tgcgctagca gtagggtgca tagatatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnngtt tttagacctg tgtggaaagt gtcaggcgtg taccatttca tttgttcagt
     9901 taaacctgcc atgagctttt cttgctcggg caaatggcgc atatgaattt tttcataacc
     9961 ttccatgatt tctttaggaa caaagggagg ggagtaagtg gcaatttggg tgactaattt
    10021 ttcaaagtca ccttctttac gcgctaacaa tttggttaat tcttgaggtt tggtaattaa
    10081 agagttgcca gcattttgat acacgcctgc ggtatcaact aacatcacac tgcgaacttc
    10141 taagaagtat aaagcggaat ataaaacagc aataccaccg cctaaagagt gacctgcaac
    10201 gtgtaagtct ttttgaatac caagagtagt gacaaattta cgtacagctt ctgctaagtt
    10261 agctaaggtg tattcaaatg ttgcaggtgc gatagtgtca ccatgaccag gtaagtcaac
    10321 agcaacaaca tgatattgtt tggtgagttg acgagcaata cgattccagt tgtcacgtga
    10381 gccagaaaac ccatggatta acagaactgt gggtgccgtt ttagagccac cttcactata
    10441 actaaaggtg aagtcttcga ctttaatggt tttgctttct aagccagccc atttgcgctc
    10501 ttgttgcaaa atagattgag ttagcgcaac agggtcagtg actaaggctg gtgctgcaaa
    10561 cgtgcttgct tgatagctaa tcgctaatag cgaggcaagt atgagttttt ttagcatatt
    10621 attattcctg tgtttgatta atcattgtgc tgagtataaa cacaggatca tgacaaagac
    10681 attgacactt gtcatgtcta tttaggccgc taaagctaat aggctctagc agccagtcta
    10741 atttttagaa gcgataacca atactcaaac cgatgacagg atataattca ctataatcag
    10801 catcgttaat ttctttttga agtgcctttt cttcgttagc cacttcttgt tgaaaaacag
    10861 gatcactggt attcacaata atgtcagtat tggtgtttct aaaactacct gtgacattta
    10921 aatctactgt aggtttatct tgaaataaaa caccaatatc gaatttggca taaatacggc
    10981 caccttgcat ggcgttaccc caaccaatac ccgcataagg cgcaccagaa ccaaagtcaa
    11041 taccagcatt aatttggcca tcaggaaagc tctgatagga ttgaccatca atatcgcagc
    11101 tttgtgggca agtggctttt aaatcaatat tattatcatg agccaaatag cctgcactca
    11161 aataaaaact accaccaaaa ggatgaatat cggtaattaa accataacta ctaaaatcga
    11221 cagttccttt atagtttgtg ccgctttctt caaagtcttc atcaactgag gctgcgctag
    11281 ctgttaaacg taaatttata tatttattta cgccaaaagt gccttgtacg ccaacaccta
    11341 aggtactcaa acctacacct acagcaacgt cagcctgagc ttgttggtaa cataagatac
    11401 ttaataatac gacagataat ttcttaaaca tgttaaacac tccttgaaat aagagggggc
    11461 atccctttat atttaatttt gatacgacaa acaatactta gtgaaaataa ttatgaaaaa
    11521 gacaaatttt tcttttcgga tagatacccg ctggtgtttg gatgaattat taaaagatgg
    11581 ttatataaat caacgagatg ctaatttagt cgcaaccaca ccacgtcctc gtgataagtt
    11641 acattggcat cctttagaag tgatagccca gtttgaattt attaacctaa aaaagccaag
    11701 ccataaatta aacttagata ccttatcggc atggttggct gataaagcag aacagcctct
    11761 atttcatatt gatcctttaa aaattaatgc aggaacggtt actgcggtga tgtcttatgc
    11821 gtttgctgag cggcatggta ttttggcggt acaggttgat aaagagtttg ttacgattgc
    11881 cagcgcacag ccatttcata atgaatggga agaaaattta caacatacct taaaaaataa
    11941 acaaattaaa agagtggtag ttaaccctgc tcacttacat cgtttttcta ttgagtttta
    12001 taaattagct aaagcaattg agggtgcaaa taatagcgat gctgttgcca gtaataccaa
    12061 taaaattggc aattttgagc aattattgga gttaggcaat accaccaatc ccgatgccaa
    12121 cgaccagcat attgtgagta ttgtagattg gctgttgcaa tatgcctttg atcaacgggc
    12181 gagtgatatt catttagaac caagacgcga cacaggtaat gtgcgttttc ggattgatgg
    12241 cgtattgcat aatatttatg agtttccagc gttaattatg aacgcagtga tttcacgaat
    12301 taaaatttta ggccgaatga atgttgctga aaaacgaaaa ccgcaagatg gtcgtttaaa
    12361 aacccgtagt cccaaaggtc aagaaattga attacgttta tctaccttgc caacggcatt
    12421 tggtgaaaaa atggtgatgc gtatctttga tcccgatgta ttggttcgtg gttttaaaga
    12481 gctaggttta gtgggtgatg actacgataa atggcaaaaa atggtgcaag agccaaatgg
    12541 cattatctta gtgacagggc caacaggttc aggaaaaaca accactttat actcgacact
    12601 taaacaattg gcaacggatg aggtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnngtttat aaagaaggtt tattaccatt acgtttatca ggcgcacaaa aagttgctaa
    12781 aggtctaacg actatcgaag aagtattgag agtagcacct ttgtcttgat gatttaatta
    12841 ttaatgactg ttgttcagta ttaggctaca taaaatgtga taaacaatac tttttttaat
    12901 gttaaaagcc catataataa atataagttt taacattaac gtgtaggacg tatgcaaaac
    12961 gcgattatcg cgtcaagtca actgtatgat ggcatatttc ttaacaaaga atggaatcgt
    13021 aatagccata aataacatgc agttatcagc atatcctatg agtggagtat ctttatatga
    13081 caactgcaac acctgacttg tttcattggt cagatgattt taacgtagat atacaggaag
    13141 tagatgagca acataaagta ttggttgatt tattaaatca actgcatcac gcgattgtcg
    13201 agcgtcgtgg taaaaccgct tcacaagaga ttttaaatca attagccgac tacacccgca
    13261 cccatttttt attagaagaa agtttaatgc gtgttctgca ttatccgggt ttagaaattc
    13321 ataaacaaca acatgaagat ttaatacaac aaatacatga attacaaaga aaaatagacg
    13381 aagagggtgc aacagttacc tttgagttat tacatttttt aaaaatgtgg ttaattcaac
    13441 acattaacga aagcgataaa cgttttggtg atttttttgc caaatcttcc catttgcaac
    13501 aatatgcaac atggaataaa gaagttgagc aaaccatgaa aaagaaaaaa tggtggtgga
    13561 aattctggta actactctcc ccaaaaagac ttttgtgcat taggggatgg gtaaacaact
    13621 ggtgcagtgg gtagttgatt tagcttctgc tccagttgtt caagtttttg gcttaattgt
    13681 tgtaattctt gttgtttatt taacagttga agcatttctt gttcatcaat atgagcgtct
    13741 agttctaaaa ataagcgttg ttggccattg gtttcggtag taaattgttc ttgcaaaata
    13801 tttactttga ctaaggcggt ggctgttgcc gtaatttttt cttctaactg atcgttgatt
    13861 aaggtgcttt gagcctgaat gtaagttcct gcttcttgtg cagcttgact ttgtaattgt
    13921 tgaatgagta aacgtttagc acttaaccga ctgtcattat cgcctaataa ataagattgc
    13981 tgataagtct tttgccaagc aaacaaaggc gtatggtata ggcaaattaa aatcaggact
    14041 aatctacacc aaaatggcat gtgcgaacct caagctnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnntttg atatttatgg tttttttatt ttttaaaaag ggtataaaag
    14221 aaaaagacca acaaaaaatg ttggtctttt tacaagaaat gctgttttat ttcttttcag
    14281 gtgtccaacc tttaggacgc tcgtaatctg cattactaaa aaacttttcg cgttgttcat
    14341 ccaaaaacac atgagtttct gcttgcatca catttaggtg attttcgtta attaacatgg
    14401 tttgatgttt gagccactct tgccatgctt gtttagaaac cgtttcaaaa atggtttggc
    14461 cttttgcgcc attaaacggt ggaaaagcta agccttccat ttcttgttga tatttacgac
    14521 acattaccat ccgcgtcatg attaagctcc tatacgactg cgtgcgcgtt gcaccgcggc
    14581 aacaacttga ttgggggctg tgccaccaat atggttacgc gcattgactg aaccttctaa
    14641 acttaaaacc tcaaacacat cttgttcaat ggtggttgag aactgttgta attcggccaa
    14701 ggtcatggca ctcaagtctt tgctggtttg tacgccataa gcaacagctt taccaacaat
    14761 ttcgtgagca tctctaaatg ccgtgccgcg ttttactaaa taatcggcta aatcggtagc
    14821 tgttgcaaaa ccgcgtaaag ccgcttcacg catggcttcg cgattaggtt taagtgcagg
    14881 aaccatatca gcaaacgcgc gtaagctcgc ctgtaaggtg tctaaagcat caaataatgg
    14941 ttctttgtct tcttgattat ctttgttgta ggctaaaggt tggcctttca ttaaagttaa
    15001 taagctaatt aaatgaccat tcacacggcc tgttttgccg cggactaatt cgggcacatc
    15061 aggatttttc ttttgtggca tgatgctcga gccagtacaa aagcgatcag gaatatcgac
    15121 aaatttaaat tgttgagagt tccacaaaat taattcttcg ctcatacgag ataaatgcat
    15181 catggttaat gaggcagcag cgcaaaactc aatggcaaaa tcacgatcag aaaccgcatc
    15241 taaagagttt tcgcaaactg cttcaaaacc taagagttca gcagtataag cacgatcaat
    15301 ggggtaggtt gttcccgcta aagccgcaga gcctaaaggc atacgattaa cgcgtttacg
    15361 gcaatcggct aaacgttcat catcacgcac caacatttca aaccaagcta agagatgatg
    15421 accaaaggtg acaggttgag cggtttgtaa atgggtaaaa ccagccataa ttgtactggc
    15481 ttcgcgttct gctaagtcta ataaacctgt ttgcagacga cgtaaaatcg cacgtgtttg
    15541 gtcaatttca tcgcgtaaat ataaacgaat atcggttgct acttggtcat tacggctacg
    15601 tcctgtatga agctttttac cagcaatacc aatgcgatca gttaagcgtg attcgatatt
    15661 catatgaaca tcttctaaat caacacgcca gtcaaattga cctgcttcaa tttcttgcct
    15721 aatcgcagtt aaaccattga taatggcatc tcgttcacta tcggttaata cgccaacttt
    15781 agccaacatc gtagcgtgag cgattgaacc ttgaatatca tgtttataca aacgtttatc
    15841 aaaattgaca gacgcggtaa actcagcaac aaaagcatca gtggcctcac taaaacggcc
    15901 accccacata gaagaagaag gtgtagtcat aacagtactg caaattaaat aaacaataaa
    15961 aaatttaact agagtataaa gcaattatgc agcaaaccgc taataatccg caaaaccaga
    16021 ctgtagatga tttcttcttg cctgatttgt gcaatatgaa agcagtattg cgcttattgg
    16081 ttgtgacaga gctactagcc gtatttttaa ctttggccga tttagagcgt ttaacgcctt
    16141 ttccgtgggc tgatttggga ttaacctctt ttttgttggc atgggttggg ttgtgtacag
    16201 caacattatt atgtgctttg cgtaagcaat taagtcgtct caaacattct tatgctgcat
    16261 ttattgtatt atttttattt ttgatgatgg tggctttttt tacctttgcg gctgaaggtc
    16321 tattattata tttgcaaata cggcatgtgg gtgaagttga tgttggcaca gccttagtgc
    16381 gtaatgtgtt aatgggcggt attattggcg gcctagtttt aagatatttt tatttacaag
    16441 aattattgtt gcgtcggcaa cgtgcagagt taacagcacg cgtacaagct ctacaggctc
    16501 gaattagacc tcatttttta tttaattcga tgaatattat cgctagttta attatggttg
    16561 atcccgataa agccgaacaa gtagttgaag atttatcaag tttatttaga gcgagtttaa
    16621 aggcagaagg tgaagtccca cttaaagatg agattgattt atgtaatcgt tatctcaata
    16681 tagaaaaact acgcttaggt aaacgtttag agtttgaatg gcgtttagat ggtttaatac
    16741 ccagtttaaa aatacctgca ttgacattac aaccgctctt agaaaatgcc atttatcatg
    16801 gtgtagagtt agatgatgaa ggtggtaaac tgactatact tgtagaatgg acaggtgaat
    16861 tggtgaatat agtcattact aatccctatc accaacataa aaagagcaaa cataaaggca
    16921 ataatatggc tacagccaat atcaaagaac gtctaaaagt ttattatggt gagcaagcga
    16981 cctttaaaag tgtcagcgcg ggcaatttat ttacaacaca aattagttat cctttaccac
    17041 aacaacaatg aatttataaa aagaaggagg ttatatgcgc atattagtct gtgacgatga
    17101 agcattagct cgagaccgtt tggtacgttt attacgtgan nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnt ctcctcgtcc tgaagcccgt aaacatatta ccgcgcgcac
    17281 acataaaggc atggaattaa ttccaattag tgaaatttat tactttctgg ctgatcaaaa
    17341 atatgtcact gtacgacata cacgtggcga aattttgatt ggtgaaacac ttaaagattt
    17401 agaagaagaa tttggccatc attttttacg gattcatcgt aatgcattgt tggcgttaag
    17461 ttatttggat ggtttagaaa ccaatccaac aggacaaact tttgtgcgtt ttcgaggtat
    17521 tcccgaaaaa ctattagtca gtcgcaggca tttaccgcta ttacgagaaa aaatgcaaag
    17581 tttgtaacta gaccctacta ttagatgaca aatagctaaa ttatcattta gaatgtgagg
    17641 gctatacatc tgtatagagc gtgtgttatt aacaggttag attttttgac tagttaataa
    17701 gctctataca aataataaaa ttaacaacac gtgcttaggc caataattag aaaatcgaca
    17761 tatatgcgtt tgagacttgt ggattagtca aagatttaag tcttaagttg tcgaaaagca
    17821 ctatcattgc taagtatttt gcccaaagct cgtcagttct attgaggctc gtttatgaac
    17881 ggctggcgta aaatcattat ctcatcttta tggcttacag ctgccgcctt gggtttatcg
    17941 ggttgtgcaa atttgcataa tgttaaaacc gaagagccag aaaaatatgt tctaggcgaa
    18001 aacgcctctg aagcccgtta cttttataca cgtagtggtt tacgtttatt tggtcaatgg
    18061 tgggttccaa ccgagcgtcc aagagctatc attttattag cacatggtac agtattacat
    18121 tctggatttt attctccgtg ggcaaacgag ttaaccaaac atggttatgc tgtatttggc
    18181 atagacctgc gtggttgggg acagtcacaa ggttatggtc gtcgtggaaa tgtcactaat
    18241 tatgatgact atgtagaaga tttaacctta gcacggcgtg aaattcgtcg tcgtttcccc
    18301 aatgttcctg tgtatttaca gggagagtca ttgggtggag cagtggtgtt gttagcacat
    18361 attatggata aagtaacaac cgacggcatt attcttaacg cgcctgcggt gcgtattaac
    18421 ccagccgctc agtggagttt gtggggaaat gcccaattaa ccaagcatat gcctgaaacc
    18481 ccattattgc ctttatttaa atctttaaca ggattagtcc tcacggatcc acaagcgaaa
    18541 gatcgttttt ggacagatcc tttaaccaca cgtacggctt taggtggtgg ttttgtcacg
    18601 gctttacaag atgcgacaga gcggttacaa ctcaatgtaa ataatgtgcg tgcgccaatg
    18661 ttgattattc atggtactaa ggattatgtg attccaaaaa gctccagtga atttttatta
    18721 aaaacagcgc aaagtaacga taaaacctta cgtattatag aaggcttaca tcattcaact
    18781 ttacatgata aacaaaaacg tcaggtatgg gatgacacaa ttgcatggtt agatcaacac
    18841 gctgaccaag ccttagctgc gcgtaaaaaa ctacctgatg aaacagaaat ttctgcattg
    18901 gaagatcaag tgcctactca agaaaaagca gcttatcgtc caaagtaccc aaaacttact
    18961 atgacaacaa cgaggaacta attaatgaaa catttattgt ggcctgtttt ggttgccacg
    19021 ttgctttcag gatgtgcaac agccgtaagc ccagttaatg gctctttata caccaatcta
    19081 cgcgcaccaa tgctagtaac agaaagtcct gataaaccga ctaaagtggg tcgtgcaaca
    19141 gtacgttcaa ttttgggaat ttatgcggta ggtgatgcga gtattgaggc tgctgcaaaa
    19201 aatgcgggta ttacgcgtat tcatcatgtt gattatcaat cacaaaatat tttaggcgtt
    19261 atgtctgatt ttaccgtaat tgtttatggt aactaagtaa ttattgacga taaagcataa
    19321 aaagcctcag aaatttgggt atttctgagg cttttttatt tagaataagc aaaatttttt
    19381 tggattattg ttatgcgaac cttacgtatt gccacacgta aaagcccttt ggccttatgg
    19441 caagctgaat atgttaaagc aagtttatta aagcaccatc cacaattaac cattgagtta
    19501 gtcactttta ccacacaagg tgacaaaatc ttagatacac cactagcaaa aattggtggt
    19561 aaaggcttgt ttgttaaaga gttagaacag gctatgttgg ctggtgatgc tgatttagca
    19621 gttcattcta tgaaagatgt gcccatggag tgtccagaag gtttagcaat taccacgatt
    19681 tgtgagcgtg aagatccaac cgatgccttt gtctctaatc gtttcgcgtc tttgcaagaa
    19741 ttacctttgg gggcaattgt cggcacatcc agtttgcgtc gtcagtgtca attacgtgct
    19801 ttacgcccag atttagaaat tcgtgatttg cgtggcaatg tgggtacacg tttagggcgt
    19861 ttggataatg gcgaatacga tgccattatt ttggccagtg caggtttaaa acgtttaggt
    19921 ttgcagcaac gtattgctca atcattaact caactattac ctgctgtagg tcagggggca
    19981 gtgggtattg aggcgcgtag taacgatact gaattattag cattattagc accgttgcat
    20041 cacttaccaa ccgcgttgtg tgtgcaagct gagcgagcta tgaaccgacg tttacaaggt
    20101 ggttgccaag tgccaattgc tggttttgct actttagata atcagcaatt aacattaaat
    20161 gccttagtag gaagtttgga tggccaatct gtattgcgtt accaagcaca aaccacagat
    20221 ttaagtacga ttgagcagct aggggaaaat gttgcagacg gcctattggc acaaggcgcg
    20281 gataaagttt tggcggcggt ttatggctaa cttagcaggg ctaaacatta ttaatactcg
    20341 gcctgttaat cgagcgcaac ctttaaccga tgcactgatg caaaaaggtg cattggtcac
    20401 cgccattcct ttggtagaaa acataccttt agctctcagt gcagagcatc ggcagtattt
    20461 attaaattta gatttatacg atgtcgtttt tgtagtcagt ccaaccgcag ccaaattagg
    20521 tttagctcta ttaagtgatt attggccaca atggccgatt gctgtacagt ggttagccat
    20581 tggtgaggct actgcagaag ttttgcatca atatggcttg caagcaatag tgccaacgca
    20641 agaaactagt gaaggcctct tacaattgcc atgtttgcaa caattgcagc aaggtcaaaa
    20701 aatgttggta ttaagagggc agggcggacg taatttagtt cgcgataatt tgcaacaaca
    20761 aggtgttaaa gtagattatt tagatttata ccaacggcaa gttcccatta atagtcagca
    20821 attatggcag cagcaaaaaa aatcgcccga tttggttgtg attaccagtg gtgagatttt
    20881 acaaagttgg caacaattgg ttggtgaaca tttttccaaa attccgatgg tagtcattag
    20941 taaacgttta gttgcaatgg ctaaagcatt gggagttatg caagtgatta ctgcagcgag
    21001 tacacgtcca gaagatattg ttcatgcaat tcaacagtgg cacaaaggtc agactcatga
    21061 tatatcagag tagtttttta gcccctaata agatgtactt ataatgagtc aaacacgtca
    21121 acccattcat gtttttccta agattacgct caagtttggt ttttgggggc gagtgacagt
    21181 tattatcgta atactcaccg taattgccag tattttgttg tattatcatg gcatgcgtta
    21241 taaaaatcgt gagctattac attatcaaga aattactcaa caattacaaa gccttaagga
    21301 gcaacaagta cattatcaaa gtaagagtat tttactaca
//
LOCUS       DAOQMB010000004        20895 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_879_length_20895_cov_12.415307, whole genome shotgun sequence.
ACCESSION   DAOQMB010000004 DAOQMB010000000
VERSION     DAOQMB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20895)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20895
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_879_length_20895_cov_12.415307"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLY05_00440"
     CDS             <1..1142
                     /locus_tag="PLY05_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01798.1"
                     /translation="PVVGNVLAGQGDTRPFEQPLPINTQPKRLALGSDFAIGVKEDGT
                     VWSWGYGMLGLGKFESRDVPQAIPNMTDFIEVVAGSEHVLALRKDGTVWSWGDNEKGA
                     LGYKEDGVPYGSPNDIRFSPHQLTPKQIPELKDIVSIAAGSRYSLALDKEGRVWGWGS
                     EFKASKTKTLTPEIVFQAENAVKVVSHDMSQSFGILTKTGQVKLWQTNKLTEVLFERP
                     VVDFVIGPVSFNMYFLLDTGRVYAVGDNRRGELGCCRLKSKTTEPVLIPQLGHIVKIS
                     TYSALDDKGRVWQWGREAGVARDYVYFSASNIYTQFYPRVMLNGKKIVDMYCCTAAFA
                     EDGSVWFWGNDLGGRRGDAKPIKSSYEEKTHKYWTTPIRSLWMWK"
     gene            1374..1628
                     /locus_tag="PLY05_00445"
     CDS             1374..1628
                     /locus_tag="PLY05_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012519136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNC01799.1"
                     /translation="MNVISFSQARSSLKAVLDGVIDDADCTIITRRDAEDAVVMSLNH
                     YNSLMETVHLLRSPANAAHLAKSINQYQQAQIVERELIDD"
     gene            1621..1887
                     /locus_tag="PLY05_00450"
     CDS             1621..1887
                     /locus_tag="PLY05_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HNC01800.1"
                     /translation="MTRLLAWTDEAWSDYVYWQAQDKKTLKRINLLITDCKRSPFEGI
                     GKPEALKENLSGFWSRRIDETNRLVYAVNNSHLTIISCRYHYQI"
     gene            complement(2029..3807)
                     /gene="aspS"
                     /locus_tag="PLY05_00455"
     CDS             complement(2029..3807)
                     /gene="aspS"
                     /locus_tag="PLY05_00455"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNC01801.1"
                     /translation="MMRTHYCGSLNESHIGQQVTLCGWVHRRRDHSHVVFFDVRDRDG
                     LLQVIIERADTPELFDAAGKMRNEYVLQITGKVRAREEHLINPDIASGKIEVLADKIE
                     ILNSSETPPFPLNDDFANVGEEIRLKYRFLDMRRPEMLQRMRFRSQVTSKIRNYLDRH
                     GFLDVETPVLTRATPEGARDYLVPSRTQQGSFFALPQSPQLFKQLLMVSGFDRYYQIA
                     KCFRDEDLRADRQPEFTQIDIETSFMSDEEIMGIAEGMAVELFDELLGIKFDKFPRMT
                     YAEAMRRFASDKPDLRVPLELVDVKDLMQEVEFKVFAGPAKDPKGRIAAIRVPQAADK
                     LTRGQIDEYTKFVGIYGAKGLAYIKVNDLSQGVAGLQSPIVKFIEPIVLELLKRVGAE
                     NGDIVFFGADKAKVVNDAMGALRVKIGHDLNMLTCEWAPLWVVDFPMFEEVGEGQWTS
                     VHHPFTMPHGTVEDLKNNPAEATSVAYDMVLNGTEIGGGSLRIYNPDMQKAVFEALGI
                     GEEEAQEKFSFLLDALKFGAPPHGGLAFGLDRLVMLMTGATSIRDVIAFPKTKTAECL
                     MTQAPAPVEGKQLRELGIRLREKVGE"
     gene            complement(3932..4471)
                     /locus_tag="PLY05_00460"
     CDS             complement(3932..4471)
                     /locus_tag="PLY05_00460"
                     /inference="COORDINATES: protein motif:HMM:NF022607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="HNC01802.1"
                     /translation="MKKFFALLLVFVLGAGFYSTPYYAAHQVQQAAQSKDSQRLNSYI
                     DYPALRTSLKESISQNFSQALLNNKTDNGMNAFAAMFASAFVTPLIDSLVTPENIALM
                     LQAELPEKLKHTDKQAAEANQQETEKDQLIMAKSYVDFDHFAVDIAHQQQPQAIFRFT
                     FTRQGLFAWKLTGLTLPKP"
     gene            complement(4471..5283)
                     /locus_tag="PLY05_00465"
     CDS             complement(4471..5283)
                     /locus_tag="PLY05_00465"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010115969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HNC01803.1"
                     /translation="MKRSVFFISDGTGITAETLGHSLLAQFGSVKFEEFTLPYIDSIE
                     RAHQAVLKINQVAAEDGCKPIIIDTLVNKEIRQIITNANGCMFDVFETFVGKLEEELG
                     VQPTNQIGKHTVNTLSYKTRIDAIHFALDNDDGARTRHYDSADIILIGVSRSGKTPTC
                     IYLAMQFGIRAANYPLTEDDLDDNCLPKSLKEHKNKLFGLMIDAERLTAIRNERKANS
                     RYASPQQCQMELRAVEGIYVKEGIPFLNVSEMSVEEIATRIIQLAGLKRRIN"
     gene            5595..7970
                     /gene="ppsA"
                     /locus_tag="PLY05_00470"
     CDS             5595..7970
                     /gene="ppsA"
                     /locus_tag="PLY05_00470"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008304115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNC01804.1"
                     /translation="MEALVLWFQQLGKNDVEIVGGKNASLGEMISNLANVGVSVPNGF
                     ATTAQAYRDFLEQSGLNKRINEALENLNVDDVVRLAEVGKEIREWIVATPLQPALEQQ
                     IRDNFAKMSENNPNIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAVKEVFA
                     SLFNDRAIAYRVHQGYDHKGVALSAGIQRMVRSETGAAGVMFTLDTESGFRDVVFITS
                     SYGLGEMVVQGAVNPDEFYLHKPTLIAKRPCILRRNLGSKTQKMIYGQTGVAGKSTLV
                     VDVDKPERLKFSISDAEAAALAEQALIIEKHYGQPMDIEWAKDGDDGQLYIVQARPET
                     VKSRQNVGTMERYLLKQKGTVVLCEGRSIGQRIGSGRVKVVNSIREMEKVQPGDVLVS
                     DMTDPDWEPVMKRAAAIVTNRGGRTCHAAIIARELGVPAIVGCGNATEVLADGMEVTV
                     SCAEGDTGFIYEGMLDFEVQKNSIESMPKLPFKIMMNVGNPDRAFDFAQIPNQGIGLA
                     RLEFIINRMIGVHPKALLNYETLPRDIRNAVTSRAAGYASPVEFYVEKLVEGIATLAA
                     AFYPQRVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYISDNFRDCFDLEV
                     RALKKVRDEMGLTNVEVMIPFVRTTSEAQRVIELLAEKGLKRGENGLKIIMMCELPTN
                     ALLAEDFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLFDERDPAVKFLLSRAIQACK
                     KAGKYVGICGQGPSDHPDLAKWLMDEGIDSVSLNPDSVLETWFFLAENHGK"
     gene            8077..11010
                     /locus_tag="PLY05_00475"
     CDS             8077..11010
                     /locus_tag="PLY05_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNC01805.1"
                     /translation="MSAPHPAFDCLRTVHIASLNVEMSEYRHKKTGASHFHLASDNPE
                     NVFLVALRTMPMDSTGVAHILEHTALCGSKRFPVRDPFFLMIRRSLNSFMNAFTSSDW
                     TAYPFASQNRQDFFNLLSVYLDAVFYARLDPLDFAQEGIRVEFSEAENPNSPLVYKGV
                     VFNEMKGAMSSPTSILSDVLNKYVFPTTTYHYNSGGDPETIPDLSYDDLIAFYKKHYH
                     PSNAVFMTFGDIPAAELQRRFEEEALHSFERSETIKGKDEKRYYAPVIVEDHYPLDEG
                     DLANKSHVVMSWLLGQTANISERLAMNLLGGVLLENSASPLRQFLETCGLGEAPSPMC
                     GLDDNGREMSFACGLEGCAADKAADIEAGILEVLQKVAVEGVAQEDVEAVLHQIELSQ
                     REIGGDSYPYGLQLVLNGLNAALQDADPLALWDVDSVLAQLRQSIQDPDYIKNLVKTA
                     LLDNPHRVRLTLKPDNTISQKRQAAEAARLAKIQQGLSEADKQAIIANTKALVERQSL
                     VDDVSILPKVGLADIPADLKIAEGKSYTLPINGIDVPITTFEAGTNGLFYQQIIIDLP
                     HLTEREQSLVPFYSSLLSELGAGEHDYLAMQQWQSQVSGGVRMGLSMRTSLNNAGQAQ
                     AHLVASCKALHYNTDSFNLLKTTLEQLRFDEAERVQDLLAQRKARFEASITDSGHALA
                     MQTASHSMSALSQLEYRISGLPALRTLRELVANSQSEDGITSLLAELQAIHHKVLAAP
                     RQFLLIAEKERLATLVETLQQTWSASSIAASVDDDVKQIQAQANPQATQIAWLANTQV
                     QFCAAAYPAVPIDHDDAPALMILGGFLRNGFLHRAIREQGGAYGGGAAYDSNACAFRF
                     FSYRDPRLADTFADFQASLEWLQSNNHEERQLEEVILGIMASLDKPMSPAGEARSAFH
                     NALYGRTSAQRRALRAKLLAVTIADLQAVAAKYLSAERMSKAVVAPYSQAETVQSLGF
                     VIERL"
     gene            11017..12033
                     /locus_tag="PLY05_00480"
     CDS             11017..12033
                     /locus_tag="PLY05_00480"
                     /EC_number="2.7.7.1"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamide-nucleotide
                     adenylyltransferase/Nudix hydroxylase"
                     /protein_id="HNC01806.1"
                     /translation="MFDFLVFIGRFQPFHIGHAKVLKQALAQSAEVIVLIGSARQPRN
                     TRNPFSFAERKAMILASLSPDEAVRVHCQPLNDCLYNDALWVKSVQEAVQVVVQQQNA
                     TKQAKIGLIGHQKDQSSYYLKLFPQWQSVDVDNHFALSATPLRDNYLLGAMPNNELLP
                     LGTQQFLAEFMANEQYQHLQAEALFIQHYKQAWDKAPYPPVFVTVDAVVVQAGHILLV
                     ERKARPGKGLFALPGGFVDQGETLIAACIRELREETRLKVPEPVLHGSLKAQQVFDEP
                     NRSARGRTITHAFKFELNPDSGLPKVKGGDDAKSAFWLPLSQLEPEQLFEDHYHIINA
                     MTGV"
     assembly_gap    12192..12291
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(12310..13641)
                     /gene="miaB"
                     /locus_tag="PLY05_00485"
                     /pseudo
     CDS             complement(12310..13641)
                     /gene="miaB"
                     /locus_tag="PLY05_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
     assembly_gap    13844..13943
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13944..15634
                     /locus_tag="PLY05_00490"
     CDS             <13944..15634
                     /locus_tag="PLY05_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC01807.1"
                     /translation="RGEWLIVLGQRQDWQNYQQFYTGLQRPQLEHKCYSYLARANTGD
                     VTALAEARQELWLNTKSLPTPCPDVLASLQQSTILSEEDYWWRLRLALQRNDKAVARS
                     LAGKLGIDLSYKILDQIYTSPSVYLAKPDVETAIGHELYLSALARYSRQNLDKAVNYW
                     QQVENRFNQEDRPYGWRILALLAAKRQDPRAVDWFKHSKTVFWPDEDKEWAIRSAIRI
                     QDWSSVLAFINLLSAEKQQERAWQYWRARSMEGDSSKSKFVQAIFNGLAVDDDYYGLL
                     AKDRLGGKIASRPQNYVVTEQDKKVVRDNVNIQRALGLYLLDLRTEAVREWNWALRNA
                     NDKFLLAAAEEAANVRWFDRAIYAAERTKQLHSYNLRYLTPYKEVVRGYTQELGVDPA
                     WVYGLIRQESRFVIDAKSVVGAGGLMQLMPSTAQWVANRLKIPYHAGMVNEVGTNVRL
                     GTYYINHVFKQLGSQPVLATAGYNAGPNRAKQWQHATQALPVDVYTESIPFLETRDYV
                     KKVMTNAVHYALGFGEGAQSITMRMGVKIPPRTGKVVESKDSQEQKQDVNTDNN"
     gene            complement(15667..16587)
                     /locus_tag="PLY05_00495"
     CDS             complement(15667..16587)
                     /locus_tag="PLY05_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC01808.1"
                     /translation="MTSNKLNVRKRTSALLSVLLVTCLQTPTTYAGNDVYFSTPVTEL
                     MDGAALDAHTSVDAAPDVPLTTEELAKIPDATPVEEPFSKYGNPTQYTVLGKTYTVMA
                     KAKNFKQVGLASWYGKKFHGQRTSSGERFDMFKLTAAHKNLPIPCYAKVTNQENGKSV
                     IVKINDRGPFHSDRVMDLSWAAAAKLEMLGKGTAKIKLEVISSADATTAVASTKPVTT
                     PTTTIAANDANKQFFVQVGAFSTEDYALSLQSKLSEVVALPIEVSSSDSNKAIHKVQV
                     GPFVDETTANKAREYILQLAAVSNPIIIKR"
     gene            complement(17053..17604)
                     /locus_tag="PLY05_00500"
     CDS             complement(17053..17604)
                     /locus_tag="PLY05_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNC01809.1"
                     /translation="MKRFQQIVLTSCLLASSISYAQNFKVTSPDIKQGKLTESQVFNG
                     FGCTGQNISPALTWKNAPKNTKSFAVTVYDPDAPTGSGWWHWVVYNIPADSQQLATGA
                     GSENSTNLPSGAVQGRTDFGSHSFGGACPPQGHGLHRYIFTVHALKVDKLELPAEASA
                     AMIGYMINANQIAKTSFTAKYGR"
     gene            complement(17618..18694)
                     /gene="mltB"
                     /locus_tag="PLY05_00505"
     CDS             complement(17618..18694)
                     /gene="mltB"
                     /locus_tag="PLY05_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HNC01810.1"
                     /translation="MSFKTLFSLLLGLSTANFSHAGDFDQYEELQALIASLEREAIYP
                     RGELNTVFSQVKRQNKALEAMMRPAEGTKEWKDYRPSFLSNERINRGLDFWDKYHEAL
                     EVAEQQFGVPQEIILAILGVETKFGGNKGGFKVLDTLTTMAFDFPRRSTFFRQELKNY
                     LILCKQQGLNPTEQLGSYAGAMGFPQFMPSSWRNLGIDFDGDNKADLINNPIDAIGSI
                     GNYFAANGWKKDEAIATRAKIIGDNYDELINTKELKTLTTLAELKAKGLDVKEGSFAE
                     NTPVSALRLQGDNGAEFWLTFNNFYVITTYNRSHLYAMAVFQLSQALKEAKQQLKTAQ
                     DALAAAPTIAPQPVVDTTTIPPAP"
     gene            complement(18701..19849)
                     /gene="rodA"
                     /locus_tag="PLY05_00510"
     CDS             complement(18701..19849)
                     /gene="rodA"
                     /locus_tag="PLY05_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNC01811.1"
                     /translation="MNQRQHYQFLRRLPDDVTQPHKRSWWLRWHIDFPLLALLLLVCI
                     VGLLELYSASGRNLALVIKQCSSLGLGLLAMAILAQIPPRIYQAISPWLYCAVVVLLI
                     MVALFGTVAMGARRWLTIPHVTRFQPSEFMKLAMPMMIAWFLAQQILPPRLKHIIVTI
                     LLIAIPAALIARQPDLGTALLVASSGFFVLFLAGLPWWFLGLSAAGLAAIAPIAWHFL
                     HDYQRQRILTLIDPQSDPLGTGWNIIQSKTAIGSGGLTGKGWLNGTQAHLEFLPEGHT
                     DFIIATYSEEFGWMGIIVLLTLYSLIIGRCLYIASHANDTYRRLLAGAISLSFFVYVF
                     VNAGMVSGILPVVGVPLPFISYGGTAMLTLMSGFGILMAIHTHQQNLD"
     gene            20006..20671
                     /locus_tag="PLY05_00515"
     CDS             20006..20671
                     /locus_tag="PLY05_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC01812.1"
                     /translation="MTANVMMAKKRDRTRDKLLIAVQELLLEKPTANLSIKDIAHQAE
                     MVHGSFYNYFESVHDVIQGVAELFLRAHACLADKITQGIDDKALVFALKTRQTLLLIG
                     QEPNYGHLLFDAGLPVDQFVMGLRLGLRIDIEQGIKQGRFVCADLELTLSLLAGGILG
                     LALDLHRHILSIERIEAATAKLLEQLGMDNKEAQQLAHYPTQFITPVTLPLSNAKLTE
                     FMR"
BASE COUNT         6178 a         3854 c         4481 g         6182 t
ORIGIN      
        1 tgcctgtagt gggtaatgtg cttgcagggc aaggtgacac gcgccccttt gagcagccct
       61 tacccattaa cacgcagcct aaacgcttgg cgttgggtag tgattttgcg attggggtta
      121 aggaagatgg cacagtgtgg agttggggct atggaatgtt gggacttggt aagtttgaaa
      181 gtcgagatgt accacaggca atacccaata tgactgattt tatagaggta gtcgcaggta
      241 gtgagcatgt attggcgttg cgtaaagatg gcacagtatg gagttggggc gataatgaaa
      301 aaggggcatt aggttataaa gaggatggtg tcccttatgg atcaccgaat gacatacgtt
      361 ttagtccgca tcaactgacc cccaaacaaa tccccgaatt aaaggatatt gtgagtattg
      421 cggctgggtc gcgttattcg ctggcgttgg ataaagaggg gagagtgtgg gggtggggga
      481 gtgagtttaa agcatcaaag actaaaacgt tgacaccaga aatagtattt caagcagaaa
      541 atgcggttaa ggttgtcagc catgatatgt cacaatcatt tggcatatta actaaaactg
      601 gccaagtgaa gctatggcaa accaataaat taacagaagt gttatttgaa cgtcctgttg
      661 ttgactttgt gataggacca gtttcattta atatgtattt tttgttagac acgggtagag
      721 tctatgcggt tggtgataat cgtagaggag agttaggatg ctgtagattg aagagtaaaa
      781 ccacagaacc agtactcata cctcagttgg ggcatattgt taaaataagt acttatagtg
      841 ccttagatga taaaggccga gtatggcaat gggggcgtga ggcaggtgtg gctagagatt
      901 atgtctattt ttcggcatct aatatttaca cacagtttta tccaagagtg atgctgaatg
      961 gtaaaaaaat agttgatatg tattgttgca cagcagcttt tgctgaggat ggctctgttt
     1021 ggttttgggg taatgattta ggaggaagaa gaggagatgc taaacccatt aaaagttctt
     1081 atgaagaaaa aacgcataaa tattggacaa cacctatccg ttcattatgg atgtggaaat
     1141 aagaattaat tagtaaaaga ccatttaata cgagagcaag tagcttgggt tgaggaacga
     1201 aacccatcat atttaattga taaatcgtgg gtttcctacg tcaatcacac gctacaacaa
     1261 gcctgtatgc agcgtagcga aatacgggtt aataaaaaac aaaatacaat ttgtataaat
     1321 atcgctattt tcactatact tgtaccatat acagtacaag tatggggcag tttatgaatg
     1381 ttatctcttt tagccaagca agaagcagcc taaaggccgt attagacggt gtgattgatg
     1441 atgccgattg caccattatt acgcgccgtg atgccgaaga tgctgtcgtt atgtcactaa
     1501 accactataa cagcttaatg gaaacagtac atttactccg ttctcctgct aatgctgctc
     1561 atttggcaaa atctattaac caatatcaac aggcacaaat tgttgaaaga gagttaattg
     1621 atgactaggc tattagcatg gacagatgaa gcatggagtg attatgtgta ttggcaggcg
     1681 caagacaaaa aaacattaaa acgcatcaat ctgctgatta cagactgtaa acgctcacca
     1741 tttgaaggaa taggaaaacc cgaagccctt aaagaaaatt tgtcaggatt ttggtcaagg
     1801 cgcattgatg agacaaatcg tttggtttat gcggttaata atagccattt gaccattatt
     1861 tcttgtcgtt atcattatca aatttaagtt ttgtagtctg tatgcagcgt agcaagtagg
     1921 atgggttgag gaacgaaacc catcatattt aattgataaa ctgtgggttt cctacagaaa
     1981 ttggacgagt tccctctcct attaggagag ggttaggttg aggtctattt actcgccgac
     2041 tttttcgcgt aaacgaatac ctaactcgcg taactgctta ccttcaacag gggcaggtgc
     2101 ttgagtcatc aaacattcag cagttttggt tttcgggaaa gcgattacat cacgaataga
     2161 cgttgcgcct gtcattaaca tcaccaaacg gtctaaacca aaggccaaac cgccatgtgg
     2221 tggcgcacca aattttaaag cgtctaacaa gaagctaaat ttctcttgtg cttcttcttc
     2281 gccaatacca agcgcttcaa acactgcttt ttgcatatct ggattgtaaa tacgcaaaga
     2341 accaccacca atctcagtac cgtttaacac catgtcataa gcaacagaag tggcttcggc
     2401 tggattgttt tttaggtctt caactgtgcc gtgtggcatg gtgaatggat ggtgaacaga
     2461 agtccattga ccttcgccaa cttcttcaaa catcgggaag tcaacaaccc ataaaggtgc
     2521 ccattcgcaa gtgagcatat tcaaatcgtg accgattttg acacgcaacg cacccattgc
     2581 atcgttaacc actttggctt tatctgcacc aaagaacacg atgtcgccat tttcggcacc
     2641 aacacgtttt aataactcta aaacaatcgg ttcaataaac ttaacgatag gcgattgtaa
     2701 acctgcaaca ccttggctta agtcattgac tttaatgtag gctaagcctt tagcaccata
     2761 aatacccaca aacttggtgt attcatcaat ttgaccacga gttaacttgt cagcagcttg
     2821 tggcacacga atcgcggcaa tacgaccttt aggatctttg gctgggccag caaacacttt
     2881 aaattcaact tcttgcatta agtctttaac gtcaaccaat tctaatggca cacgtaagtc
     2941 tggtttgtca gacgcaaaac gacgcatcgc ttcggcataa gtcatgcgtg ggaatttatc
     3001 aaacttaatg cctaataatt cgtcaaataa ctcaaccgcc atgccttcag caatgcccat
     3061 aatttcttca tcagacataa atgaagtttc gatgtcgatt tgggtaaatt cgggttgacg
     3121 gtctgcacgt aagtcttcgt cacggaaaca tttagcaatt tggtaataac gatcaaaacc
     3181 cgataccatc aacaattgtt taaataattg gggtgattgt ggtaaagcga agaaagaacc
     3241 ttgttgggta cgtgacggca ctaaatagtc acgtgcgcct tcaggtgtag cgcgcgttaa
     3301 tacaggtgtt tcaacatcca aaaagccatg acgatctaaa tagttacgga ttttagaggt
     3361 cacttgggaa cggaaacgca tacgttgtaa catttctgga cgacgcatat ccaagaaacg
     3421 gtattttaag cgaatctctt caccaacatt ggcaaaatcg tcatttaagg ggaatggtgg
     3481 cgtttcggag ctatttaaaa tctcaatttt atcggcaaga acttcaattt taccactagc
     3541 aatgtctggg ttaattaagt gttcttcgcg agcacgcact ttaccagtaa tttgtaatac
     3601 atattcgttg cgcattttac cagcagcgtc gaataattca ggtgtatctg cacgctcaat
     3661 aatcacttgt aataaaccat cacggtcacg aacatcaaaa aataccacat gactatggtc
     3721 acggcggcga tgaacccaac cacataaagt aacctgttga ccaatatgag attcgtttaa
     3781 actgccacaa taatgtgtgc gcatcataat tatttcacct aagctatata tttatctaat
     3841 caatgaactc tcattccata agtgtttggt gagagattct taaaaataaa agtccagcat
     3901 tatgcctgat aatgttggct ttgtgtatgt tttaaggttt gggcagtgtt aagcctgtta
     3961 atttccaagc aaataaacct tgtcgagtaa aggtaaagcg aaaaatggct tgtggctgtt
     4021 gttgatgtgc aatatccacc gcaaaatgat caaaatccac atacgacttt gccataatta
     4081 actgatcttt ttccgtttct tgttggttgg cttcggctgc ttgtttgtcg gtatgtttga
     4141 gcttttcagg aagttcagcc tgtaacatta aggcaatgtt ttcgggtgtc accaaactat
     4201 caattaatgg cgtgacaaag gcacttgcaa acattgccgc aaaggcattc atgccattat
     4261 cggttttgtt gttgagtaat gcttggctaa aattttggct aatcgactct tttaggctgg
     4321 tgcgtagggc aggataatca atataactgt ttaagcgttg actatctttg ctttgcgcag
     4381 cttgttgtac ttggtgtgcg gcataatagg gcgtgctata aaagccagcc cccaacacga
     4441 ataccaataa taaagcaaaa aattttttca ttagttaatc cgtcgtttta agcctgctag
     4501 ttgaataatg cgagtggcga tttcttcaac agacatttca gaaacattta aaaaaggaat
     4561 tccctcttta acataaatgc cttctacagc gcgtaactcc atttggcatt gttggggact
     4621 ggcataacgg ctatttgctt tgcgctcatt acgaatggct gttaaacgtt cggcatcaat
     4681 catcaaacca aagagtttat ttttgtgttc tttaagagat tttggcaaac aattgtcatc
     4741 taaatcatct tcggttaagg gataatttgc ggctcgaatg ccaaattgca tcgccaaata
     4801 aatacacgtt ggtgttttac cagaacgaga aacaccaatt aaaataatat ccgcagagtc
     4861 gtaatgacgg gttcgtgcgc cgtcgtcatt atctaaagca aaatgaatag catcaattcg
     4921 tgttttgtag gataaggtat tgacggtatg tttgccgatt tgattggtag gttgtacgcc
     4981 taattcttct tctagtttgc caacaaaggt ttcaaagaca tcaaacatac agccattagc
     5041 attggtaata atttgacgaa tttctttatt gactaaagtg tcaataataa tcggtttaca
     5101 gccgtcttca gcggcgactt gattaatttt aaggactgct tgatgagcac gttcaatact
     5161 atcaatatag ggcagggtaa attcttcaaa ttttacacta ccaaattgag ccagaagact
     5221 gtgaccaagt gtttcggcgg taatccctgt gccatcagaa ataaaaaata cactacgttt
     5281 catgttttgt tatcctttat cgtttgtact aggcgaaaga agtctttata ttatggccta
     5341 aagccaacgc tggccagtct aaatgataag acttatgttt ttctttgaaa ttaacgctat
     5401 ttgcgactat aaaattgtga gaagcgatta aagtatgcgc aatttggtga taatcacatg
     5461 actttgaata gactgaaaaa agttttatat atgctttgtg ttaatggcga cgcagactga
     5521 attgtgtagc atggtttatt atgtaatata gaccggttat ttattaattt tcatgcaacg
     5581 tagggagtga gaccttggaa gcattagtat tgtggtttca acaattaggt aaaaatgatg
     5641 ttgaaattgt aggcggcaaa aacgcctctt taggtgaaat gatcagtaat ttggctaatg
     5701 ttggggtatc tgttcccaat ggttttgcca caacagctca agcttaccgt gattttctag
     5761 agcaaagtgg cttaaataag cgcattaatg aagctttaga aaacttaaat gttgatgatg
     5821 tggtgcgttt ggctgaagta ggtaaagaaa ttcgtgaatg gattgtggcc acaccattac
     5881 aacctgcttt agaacagcaa attcgtgaca attttgccaa aatgtccgaa aacaacccaa
     5941 atattgcggt agcggtacgt tcttcagcca cagccgaaga tttgccagat gcctcttttg
     6001 cgggtcaaca agaaactttc ttaaacatcc gtggtattga taacgtttta attgccgtta
     6061 aagaagtatt tgcctcttta tttaatgatc gtgcgattgc ttatcgtgtt caccaaggtt
     6121 atgaccacaa aggcgtggca ttatcggcag gtattcaacg tatggtgcgt tccgaaactg
     6181 gcgcagcagg ggttatgttt accttagata ctgagtctgg tttccgtgat gtggtgttta
     6241 ttaccagttc ttatggttta ggtgaaatgg tggtgcaggg tgccgtaaac cctgatgagt
     6301 tttatttaca taaaccaacc ctaatagcta agcgtccttg tattttgcgc cgcaacttag
     6361 gtagcaaaac ccaaaaaatg atttatggtc aaactggtgt ggctggtaaa tcaaccttag
     6421 tggttgatgt tgataagcct gagcgtttga agttttcgat tagtgatgcc gaagcagcag
     6481 cattagcaga gcaagcgttg attatcgaaa aacattatgg ccagccgatg gatatcgaat
     6541 gggcaaaaga tggtgatgat ggtcagttat atattgtgca agcgcgtcca gaaaccgtaa
     6601 aaagccgtca aaacgtcggt acgatggagc gttatttgct taagcaaaaa ggcactgttg
     6661 tgttgtgtga aggccgctct attggtcaac gtattggttc tggccgtgtc aaagtggtta
     6721 actccatccg cgaaatggaa aaagttcaac caggtgacgt tttagtttct gatatgactg
     6781 atcctgattg ggaaccagtg atgaagcgtg cggctgcgat tgtgactaat cgtggtggtc
     6841 gtacttgcca cgcagcgatt attgcccgtg aattaggtgt gcctgcaatt gtcggttgtg
     6901 gtaatgcgac tgaagtgtta gccgatggta tggaagtgac ggtgtcttgt gccgaaggtg
     6961 atacaggctt tatttatgaa ggcatgttag attttgaagt gcaaaagaac tccattgaat
     7021 ctatgccaaa gttaccgttc aaaattatga tgaatgtggg taaccctgat cgtgctttcg
     7081 actttgctca aatccctaat caaggtattg gtttagcacg tttagagttc atcattaatc
     7141 gaatgattgg tgtgcatcca aaagccttat taaactatga aactttaccg cgtgatattc
     7201 gtaatgcggt tactagccgt gctgcgggtt atgcctcacc tgttgagttt tatgtcgaaa
     7261 aattagtgga aggtattgca acgttagcag cggccttcta cccacaacgt gtgattgtgc
     7321 gtatgtctga ctttaagtca aacgaatatg ccaatttgat cggtggtaag ttatacgagc
     7381 cagaagaaga aaacccaatg ttaggtttcc gtggtgcgtc acgttatatt tctgacaatt
     7441 tccgtgattg ttttgattta gaagtgcgtg ccttgaaaaa agtccgtgat gaaatgggct
     7501 taactaacgt tgaagtaatg attccttttg ttcgtaccac ctccgaagca caacgtgtga
     7561 ttgagttgtt ggccgaaaaa ggcttaaagc gtggcgaaaa tggtctcaaa atcatcatga
     7621 tgtgtgagtt accaaccaat gccttattag cagaagactt cttagagcac tttgacggct
     7681 tctcgattgg ttctaacgat ttaacccaat taaccttggg cttagaccgt gactcaggca
     7741 ttgtttcaca cttatttgat gagcgtgatc ctgcggttaa gttcttattg tctcgtgcaa
     7801 ttcaagcctg taaaaaagca ggtaagtacg tgggtatctg tggtcaaggc cccagcgacc
     7861 acccagacct cgctaagtgg ttaatggatg aaggcattga ttccgtgtct ttaaatccag
     7921 actctgtgtt agaaacttgg ttcttcttag ctgagaatca tggtaagtaa gcattaggta
     7981 aattttactt gatgtatgat gtaagcctct tcggaggctt atgttttttc aattgctatg
     8041 aaataatttg cccaaataaa tatatcggag tttaccgtgt cagcaccaca tcctgccttt
     8101 gactgtttac gcactgtgca tattgcctcg ttaaacgtag aaatgagtga atatcgccat
     8161 aaaaaaacag gggcaagtca ttttcattta gcctccgata accccgaaaa tgtgtttttg
     8221 gtggccttac gcaccatgcc gatggattca acaggcgtag cccatatttt agaacacacg
     8281 gctttatgtg gttctaaacg ttttcctgtg cgcgatccgt tttttttaat gattcgtcgc
     8341 tccttaaatt cctttatgaa tgcgtttacc tctagcgatt ggacagcgta tccttttgcg
     8401 agccaaaacc gtcaagactt ttttaatttg ttgtctgtgt atttagatgc ggtgttttat
     8461 gcccgtttag accccttaga ttttgctcaa gaaggcattc gtgttgagtt tagtgaagca
     8521 gaaaatccga atagtccttt agtgtacaag ggtgttgtct ttaacgaaat gaaaggggcg
     8581 atgagttcac ccaccagtat tttgtcagat gtattaaata aatatgtttt tccaaccact
     8641 acctatcatt acaactcagg tggcgacccc gaaaccattc ccgatttaag ttatgacgat
     8701 ttaatcgctt tttataaaaa gcattatcac cccagtaatg ccgtatttat gacctttggt
     8761 gatattcctg cggctgaatt acaacgccgt tttgaagaag aagccttaca cagttttgag
     8821 cgtagcgaaa ccattaaagg taaagatgaa aaacgttact acgcgcctgt catcgttgaa
     8881 gaccattatc ctttagatga aggtgattta gccaataaaa gtcatgtggt gatgagttgg
     8941 ttattaggtc aaaccgcgaa tattagcgaa cgtttggcga tgaatttgtt gggtggtgtg
     9001 ttattagaaa attcagcttc acccttacgc caatttttag aaacttgtgg cttgggcgaa
     9061 gcgccatcac ccatgtgtgg tttagatgat aatggccgtg aaatgagttt tgcttgtggc
     9121 ttagaaggct gtgctgccga taaagccgcc gacattgaag caggtatttt agaagtccta
     9181 caaaaagttg ctgtagaagg tgtagcccaa gaagatgtag aagctgtttt gcatcaaatt
     9241 gaattatctc aacgcgaaat tggtggcgac tcttatcctt atggtttgca attagtgtta
     9301 aatggtttaa atgctgcatt acaagatgct gaccccttag ccttgtggga tgttgattca
     9361 gttttggcac aattacgcca gtccattcaa gaccctgact atattaaaaa cctcgtcaaa
     9421 accgctttgt tggataatcc acaccgtgtg cgtttgacct taaaacccga taataccatt
     9481 agccaaaaac gtcaagcagc cgaagccgct cgtttagcca agattcaaca aggcttaagt
     9541 gaggcagata aacaagcaat tattgccaat accaaagcct tggtagaacg ccaatcactc
     9601 gttgatgatg tgagtatttt gcccaaagtg ggtttagcgg atattcctgc tgacttaaaa
     9661 attgccgaag gtaaatccta tacactccct ataaatggga ttgatgtacc cattaccacc
     9721 ttcgaagcag ggacgaatgg tttgttttat caacaaatta ttatcgattt accccattta
     9781 accgaacgtg agcaaagcct tgtgccgttt tatagtagct tgttgagtga attgggggca
     9841 ggtgagcatg attatttagc catgcagcaa tggcaatcac aagtcagtgg tggggtgcgt
     9901 atgggtttaa gtatgcgtac cagtctgaat aatgcagggc aagctcaagc tcatttggtc
     9961 gcttcatgca aagccttaca ttacaacacc gatagcttta acttattaaa aacgacttta
    10021 gagcaattgc gctttgatga agccgaacgt gtgcaagatt tattggcaca gcgtaaagcc
    10081 cgttttgaag cgagtattac cgatagtggt catgccttgg caatgcaaac agctagtcat
    10141 agcatgagtg ctttaagcca attggaatac cgtatttcgg gtttgcccgc gttgcgtaca
    10201 ttgcgtgagt tagtcgccaa tagccaaagt gaagacggca taaccagtct attagcagag
    10261 ttacaagcca ttcatcacaa agtcttagcc gcacctcgtc agtttttgtt gattgccgaa
    10321 aaagaacgtt tagcgacttt ggttgaaact ttacaacaaa cgtggtctgc gagttctatt
    10381 gctgcatcag ttgatgatga tgtgaaacaa attcaggcac aggctaatcc acaagccaca
    10441 caaatagcat ggttagcaaa tactcaagtg caattttgtg cagcagctta tccagctgtg
    10501 ccaattgacc atgacgatgc gccagcattg atgattttag ggggcttttt gcgtaatggc
    10561 tttttacatc gtgctattcg tgaacaaggt ggtgcgtatg gtggcggtgc ggcttatgac
    10621 agtaatgctt gtgcgttccg tttcttttct tatcgtgacc cacgtttggc cgatactttt
    10681 gccgatttcc aagccagttt ggagtggtta caaagcaata accacgaaga acgccaatta
    10741 gaagaagtga ttttgggcat tatggccagt ttagacaaac ccatgtcacc cgcaggcgag
    10801 gcacgtagtg ctttccataa tgccttgtat ggacgtactt ctgctcaacg tcgtgcttta
    10861 cgggctaaat tattagcggt gacgattgct gatttacaag cggtggctgc taaatatctc
    10921 agtgcagaac gtatgagcaa agcagtcgtc gcgccataca gccaagctga aactgtgcaa
    10981 agcttaggct ttgtgattga aaggttgtaa gcgaaaatgt ttgacttttt agtgtttatt
    11041 ggtcgatttc aaccatttca tattggtcat gctaaagtct taaaacaggc tttagcacaa
    11101 agtgctgaag tgattgtatt aattggctcg gcgcggcaac cacgtaacac ccgtaatccg
    11161 tttagttttg ctgagcgcaa ggcgatgatt ttagcgagct tgtcgcctga tgaagcggta
    11221 agagtgcatt gccaaccctt aaacgattgt ttgtataacg atgccttatg ggtaaaaagt
    11281 gtgcaagaag cagtgcaagt tgttgttcaa caacaaaacg caactaaaca agcgaaaatt
    11341 ggtttgattg gccatcaaaa agatcaaagc tcttattatt taaaattatt cccacaatgg
    11401 caaagtgtgg atgttgataa tcattttgcc ttatcagcaa cccctttgcg tgataattat
    11461 ttgttggggg ctatgcccaa taatgagttg ttgccattgg ggacacaaca gtttttggct
    11521 gagtttatgg ccaatgaaca atatcagcat ttacaagcag aagcgttgtt tattcaacac
    11581 tataaacaag catgggataa agcaccatat ccacctgtgt ttgtcacggt agatgctgtg
    11641 gtagtgcaag caggacatat tttattggtt gaacgcaaag caaggccggg taaaggcttg
    11701 tttgctttac caggtggatt tgttgatcaa ggggaaacgt taattgctgc gtgtattcgt
    11761 gagctacgtg aagaaacacg tttaaaagtg ccagaacctg tattacatgg ttcattaaaa
    11821 gctcaacaag tgtttgatga accaaaccgt tctgcacgtg gtcgaaccat tacccatgct
    11881 tttaagtttg agttaaatcc tgattcaggc ttaccaaaag taaaaggagg ggatgatgca
    11941 aaaagtgcgt tttggttgcc tttgtcacaa ttagagccag aacagttatt tgaggatcac
    12001 tatcatatta ttaatgcaat gacaggtgtt taagatgagg acttacgcag ttatcgtgta
    12061 ttgactcgct aatttcgtaa gtccttaaat ccgatcttat ggcttttgtt catcagtgat
    12121 ttggagggac taactctcgt tattctgatc caaatcattc catccaaaca atttcatgac
    12181 cacgcaccgt annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcgttattc
    12301 tgatccaaat cattccatcc aaacaatttc atgaccacgc accgtattga tactcataat
    12361 ttggtcaatg acaacatcag caaatttgcc aattaaggca tcattacgcg gtaataacac
    12421 ttggcgggta ttttcggcac taccaaccat atagtcttga tctcggtcag aaactttctc
    12481 aaccaatacc cgttgagttg tacctaacat tgcacgggtt ttacgttcag tattttgttt
    12541 aatgcgtgct ttaagaatag ctaaacgttc ttttttatct tcttcagaag tattgtcagg
    12601 taattcagaa gctggtgtac caggacgttt agaataaatg aagctgtaag agtggtcata
    12661 atccacttca gcaattaaat tcatggtatc gacaaaatct tggtcagttt cattggggaa
    12721 accaataata aagtctgaag acaaatgaat gtctggacga gcaattttga gcttacgaat
    12781 acgttgtaaa taatcttcag cagtatggtt gcgcttcatc gcggctaaaa tggtgtctga
    12841 acccgattgc acaggtaaat gcacatgaga cactaatttt ggcaataagg tataggcttc
    12901 aattaaatcg tcattaaatt cgaggggatg agaggtggta aaacgaatac gaccaatgcc
    12961 atcaatatca gcaatatgtt ttaacaattc agcaaaggta cacacttcac catcatgata
    13021 atgaccacga taaccattga cattttggcc taatagcgtc acttcgcgta cgccttgact
    13081 ggctaaatgc acaatttcgg caatcacatc atcaaaagga cgagagactt cttcgccacg
    13141 ggtataaggt acaacacaga aagagcaata tttagagcag ccttccataa ttgacacata
    13201 agctttaaag ccttcaactt tgggttcggg taaaaagtcg aatttctcaa tttcggggaa
    13261 gctaatatcg acaatcgaaa ttttattatt ttgtttttcg tcaagcatct ttggtagacg
    13321 atgtaaggtt tgcgggccaa acaccatatc aacataagac gcacgtttac gaatcaattc
    13381 gccttcttga gaagcaacgc agccaccaac accaatgacc acttcaggac gcttttcctt
    13441 gagtttgcgc caacgaccta actccgaaaa cactttttct tgggcttttt cacgaataga
    13501 gcaggtattc attaacaata cgtcggcttc ttcgggatta tccgtgcgca ccatgccatg
    13561 agattcacct aataaatcag ccatacgcga gctatcgtac tcattcattt ggcagccttg
    13621 ggtttcgata taaacttttt tagacatggt cgtgacgaat aaacaacaaa aaatgggtgt
    13681 gtattataac tatcttatta ggctaaaagt agatgtcttg ttacatagct tatctattat
    13741 taataaacca ataacgttta ttattaatta agttaatgat tttaaagact tatagaaaat
    13801 aaggttttta aattaaagga ggtggctatg ttaaaaagtg tgannnnnnn nnnnnnnnnn
    13861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13921 nnnnnnnnnn nnnnnnnnnn nnntacgtgg tgaatggtta attgtgttgg gacagcgtca
    13981 agattggcaa aattaccaac aattttatac agggctgcaa cgtccacagc tagagcataa
    14041 atgttatagc tatttggcaa gagcaaatac aggtgatgtc acagcattag ccgaagcccg
    14101 tcaagaacta tggttaaata ccaaaagttt gccaacgcct tgtcctgatg tattagcctc
    14161 tttacaacaa agtaccattt taagcgaaga agattattgg tggcggttgc gtttggcttt
    14221 gcaacgtaat gataaagcgg tagcacgaag tttagcaggt aaattaggca ttgatttatc
    14281 gtataaaata ttagaccaaa tttataccag ccctagtgtt tatttagcta aaccagatgt
    14341 tgaaacagcc attggccacg aattatattt atctgctttg gcgcgttatt ctcgtcaaaa
    14401 cctagataaa gccgtcaatt attggcaaca ggtagaaaat cgttttaatc aagaagatcg
    14461 accttatggt tggcgtattt tggccttatt agcagctaaa cgccaagacc cacgcgctgt
    14521 tgattggttt aaacatagta aaacggtttt ttggccagat gaagacaaag aatgggcgat
    14581 tcgtagtgcg attcgcattc aggattggag cagtgtttta gcctttatta acttattatc
    14641 cgcagaaaaa caacaagagc gtgcttggca atattggcgt gcaagatcaa tggaaggcga
    14701 tagtagcaaa agtaaatttg tccaagcaat ctttaacggt ttagctgttg atgatgatta
    14761 ttatggttta ttggctaaag accgtttagg tggaaaaatt gcgtctcgac cccaaaatta
    14821 cgttgtcact gagcaagata aaaaagtagt gcgtgataat gtaaatatcc aacgtgcttt
    14881 aggtttgtat ttattagatt tgcgtaccga ggctgtacgc gaatggaatt gggcattacg
    14941 taatgccaat gacaaatttt tattagcggc tgccgaagaa gcggctaatg tacgttggtt
    15001 tgaccgtgct atctatgccg ctgaacgtac taaacaatta catagttata atttgcgtta
    15061 tttaacacct tataaagaag tcgtgagagg ttatacgcaa gaattaggtg ttgatccagc
    15121 ttgggtgtat ggtttaattc gccaagaaag ccgttttgtg attgacgcga aatcggtggt
    15181 tggtgcaggt ggtttaatgc aattaatgcc aagcaccgca caatgggtcg ctaatcgtct
    15241 aaaaattccc tatcatgcag gcatggtaaa tgaagtagga acaaatgttc gtttaggtac
    15301 ttattatatt aatcatgtgt ttaaacaact aggtagtcaa cctgtgttag cgacagcagg
    15361 ttataacgca ggaccaaacc gagctaaaca atggcaacat gcgacgcaag cattgccagt
    15421 tgatgtttat actgaaagta ttcctttttt agaaacacgc gattatgtta aaaaagtgat
    15481 gaccaatgcg gtacattatg ctttgggttt tggcgaagga gcgcagtcta ttaccatgcg
    15541 aatgggagta aaaattcctc ctagaacagg taaagttgtt gagtcaaaag attctcaaga
    15601 gcaaaaacag gatgttaata ctgataataa ttgataaaaa ataagggctt ttaaaagccc
    15661 ttatttttaa cgtttgataa tgataggatt gctaacggca gcaagttgta agatatactc
    15721 acgtgctttg ttggcggtag tttcgtcaac aaatgggcca acttgtactt tgtgaatggc
    15781 tttattgctg tcagagctac tgacttcaat aggcagggca accacttcag ataatttgct
    15841 ttgtaagctt agagcataat cttctgtgct aaacgcgcca acttgtacaa aaaattgttt
    15901 gtttgcatca tttgcagcaa tagtcgttgt tggcgttgta acgggcttgg tgctcgcaac
    15961 agccgtagtt gcatctgcac tgctaataac ttcgagtttg attttggctg tacctttgcc
    16021 aagcatttca agtttagcag ccgcagccca tgacaaatcc attactcgat cactgtgaaa
    16081 agggccacgg tcattgattt taacaatcac acttttgccg ttttcttgat tagtgacttt
    16141 ggcataacaa ggaataggta aatttttgtg agctgctgtc aacttaaaca tatcaaaacg
    16201 ttcaccgctt gatgtgcgtt gaccatgaaa ttttttacca taccaagatg ctaaaccaac
    16261 ctgtttaaag tttttggctt tggccattac ggtataggtt ttgcctaata cggtgtattg
    16321 tgttgggttg ccatatttac taaatggttc ttcaacgggg gtagcatctg gaatttttgc
    16381 taactcttca gttgtcaatg gcacatcagg tgcagcatcg acggaagtat gagcatccaa
    16441 agcagctcca tccattaatt cagtaacggg tgtagaaaaa tacacatcat taccagcata
    16501 agtggtagga gtttgtaagc aagtaaccag caaaacgctt aataaagcag aagtgcgctt
    16561 acgtacattt aatttgttag atgtcatcgg tatctctcct aagacattgt tctggttttt
    16621 aaatctcact cagcctattt agcctaggtg gctaaattaa tttactaagt gcaatttaag
    16681 cccagtcatg cagaacacgt tgacgactat ctttacccag tgtgtaagat aatctgcata
    16741 attttgctga ttaaataatc agtaaacgat gccgaagccg tgtgtcctaa caaagttaaa
    16801 gactatgccc aaaatgaata ttttttatta gttgggcggc gatgtgggga tgctaacggt
    16861 tttttaggca tttgtctaag ttttttgttt aacaattagg catttttatg cttaaaacat
    16921 gccaaaaatg caaataaatg tttcaatgta agtaaaatgt gtctaaaaat acagcaattg
    16981 agcgttgtgt tgtgcttgag taactgatga tgagtgatta gaaaaatccc tcttttttag
    17041 agggattttt gtttaacgac cgtatttggc cgtaaagcta gttttggcaa tttgattagc
    17101 attaatcata tagccaatca ttgccgcact ggcttcggct ggaagttcta atttatcaac
    17161 tttgagagca tgaacggtga aaatgtagcg gtgtaagcca tgaccttgag ggggacaagc
    17221 accaccaaaa ctgtggctac caaaatcggt acggccttga acagcaccgc tgggtaaatt
    17281 agtactattt tcgctaccag cgcctgtggc taattgttga ctatcagcag ggatgttata
    17341 aacaacccaa tgccaccagc ctgagcctgt tggtgcatca gggtcataaa ctgttacagc
    17401 aaaacttttt gtgttttttg gggcattttt ccatgttaag gcaggagaaa tattttgacc
    17461 tgtacaacca aaaccattaa acacttggct ttctgttagt ttgccttgtt tgatgtcagg
    17521 gctagtgact ttaaagtttt gtgcataact aatgctgctt gctaataaac agctggttaa
    17581 gactatttgc tggaaacgtt tcataatcat atcttcttta tggtgcaggg gggatggttg
    17641 tagtgtcaac aacgggttga ggtgcaatcg ttggcgcggc tgctaaggca tcttgtgccg
    17701 tttttagttg ttgtttggct tcttttaaag cttggctgag ttgaaaaacg gccattgcat
    17761 ataaatgact acgattgtag gtagtaatga cataaaagtt attaaaagtc agccaaaact
    17821 ctgcaccatt atcaccttgt aatcgtaagg cagaaacagg tgtgttttct gcaaaactac
    17881 cttctttgac atctaagcct tttgccttta attcagctag ggtggttaag gtttttaatt
    17941 ctttggtatt aattaactca tcatagttgt cgccaataat tttggcacga gtggcaatag
    18001 cttcgtcttt tttccaacca ttagcagcaa aatagttgcc aatcgaacca atagcatcaa
    18061 taggattatt aattaaatcg gctttgttat caccatcaaa gtcaatacct aaattacgcc
    18121 aactcgaagg cataaactgc ggaaagccca ttgcgcctgc ataagagcca agttgctcag
    18181 ttgggtttaa gccttgttgt ttgcataaaa ttaaatagtt ttttagctct tgtctaaaaa
    18241 aagtgctacg acgcggaaaa tcaaaagcca tcgtggttaa ggtatctaat actttaaagc
    18301 cacctttgtt gccaccaaat ttagtttcta cccctaaaat agccaaaata atttcttgag
    18361 gaacgccaaa ttgttgttca gcaacttcta atgcttcgtg gtatttatcc caaaaatcca
    18421 atcctctatt aatacgctca ttgcttaaaa aagaagggcg ataatctttc cattcttttg
    18481 tgccttcggc tgggcgcatc atggcttcaa gggctttatt ttgacgttta acttgactaa
    18541 aaacggtatt caattcgccg cgcggataaa tagcttcacg ttctaaactg gcaataaggg
    18601 cttgcagctc ttcatactgg tcaaaatcgc ctgcatgact aaagttagca gtgctgagtc
    18661 caagtaacag gctaaataaa gttttaaacg acattatcca ctaatccaaa ttttgttgat
    18721 gtgtatgaat ggccattaaa ataccaaagc cagacatcag agttaacatg gctgtgccgc
    18781 cgtaactaat aaaaggcagt ggcacaccaa caacgggcaa tataccacta accattccag
    18841 cattaacaaa gacataaaca aaaaaagata ggctaattgc gccagctaat aatcgacgat
    18901 aggtgtcatt ggcatggcta gctatatata aacaacgacc aataattaag ctataaagtg
    18961 ttaataagac tataatcccc atccagccaa attcttcgga ataggtagca ataataaagt
    19021 ccgtatgacc ttcgggtaaa aactccaaat gagcttgtgt gccatttagc caacctttgc
    19081 ctgtaagtcc gcctgagcca atggctgttt ttgactgaat gatattccaa cctgttccta
    19141 aagggtcaga ctgtggatca attagagtca aaatacgctg gcgttgataa tcatgtaaaa
    19201 aatgccatgc aatgggagca attgcagcta agcctgctgc gcttaggcca agaaaccacc
    19261 acggaagtcc tgctaaaaat aagacaaaaa aaccgctact tgccacaagt aatgctgtgc
    19321 ctaaatcggg ttgacgagca atgagagctg caggaatggc aattaataaa atagttacaa
    19381 taatatgttt taaacgaggc ggtaaaattt gttgtgccaa aaaccatgca atcatcattg
    19441 gcatggcaag tttcataaat tcggaaggtt gaaaacgagt gacatgagga atggttaacc
    19501 aacgccttgc acccattgct acagtaccaa ataaagcaac cataattaat agcactacaa
    19561 cggcacaata taaccacggt gaaatagctt gataaatgcg tggaggaatt tgcgctaaaa
    19621 tcgccattgc gagtagtcct aaacctaagc tagaacattg ctttattact aaggccaaat
    19681 tacgtcctga agcactgtat aattctaata aaccaacaat acagactaat aacaataacg
    19741 ctaataatgg gaaatcaata tgccaacgta accaccatga acgtttatgc ggttgagtga
    19801 catcatcagg cagacggcgt aaaaattgat agtgttgacg ttgattcata agttttgtca
    19861 gtgtgtttga aaaagacttt gtgggcagca gcctctaact taccttgaat ggtaatcaat
    19921 cacaagtgca ggtatttttt catactacga taattttgac aaaaagttaa aaatgacata
    19981 atatcaaaaa tagaggtatt caacaatgac agcaaatgta atgatggcca aaaagcgtga
    20041 tcgcactcgt gataagttat taattgctgt acaagagtta ttattagaaa aacctacggc
    20101 aaatttgtca attaaagata ttgctcatca agcagagatg gtacatggct ctttttataa
    20161 ttattttgag tcggtgcatg atgttataca aggtgttgca gagttatttt tgcgtgcgca
    20221 tgcttgtctg gccgacaaaa tcactcaggg gattgatgat aaagcactgg tttttgcatt
    20281 aaaaacacgt caaaccttat tgttaattgg ccaagagcca aactatggtc atttattatt
    20341 tgatgctggc ttgcctgtcg atcaatttgt catgggttta agattgggtt tacgaataga
    20401 tatagaacaa ggtattaaac aaggacgttt tgtttgtgcc gatttagagt tgacattgag
    20461 ccttttggca ggtgggattt tgggcttagc tttagattta catcgacata ttttatctat
    20521 tgagcgtatt gaagcagcaa ctgctaaatt attagaacaa ttaggtatgg ataataaaga
    20581 agcgcaacaa cttgctcatt atccaacaca gtttattacg cctgtaacat tacctttatc
    20641 aaatgcaaaa ttaacggaat ttatgcgtta aaacatttta gggcagccat tcttgacttg
    20701 tacgatcaat aactaaaata actgagtgat aaaaaccata tattttgtgt ggttataata
    20761 gagggtatcc atgatgcaga aaaaaacaaa aatacgaggt tcacagttta aacgagatga
    20821 tttaggctat gaacaagccc gacgtgaggc aacatggaat gccaaaatgg tcgatcgtta
    20881 tcctgatttg attat
//
LOCUS       DAOQMB010000005        20366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_911_length_20366_cov_14.531042, whole genome shotgun sequence.
ACCESSION   DAOQMB010000005 DAOQMB010000000
VERSION     DAOQMB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 20366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20366
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_20366_cov_14.531042"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..1199
                     /locus_tag="PLY05_00520"
     CDS             135..1199
                     /locus_tag="PLY05_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HNC01813.1"
                     /translation="MIDETIKNLPVLVTGASGYIASWIVKYLLEEGYTVHATVRDPSK
                     QSSVGHLLKIAQQAKGTLKLFKADLLTEGSYAEAMQGCGVVMHTASPFVLDGFTDANE
                     ALVRPALEGTRNVLNSVNQCETVKRVVLTSSVAAIYGDNEDILRVPSGIFTEDHWNVT
                     SSVSHNPYQYSKVVAEREAWKMQGNQSRWDLVTINPSMVFGPSLTSGSQSASIDTLVQ
                     MGDGRLRMGVPKLILGIVDVREVAKAHILAAFTPQAKGRHILSNTELTMLQIGKILRK
                     QYGNKYPFPFMEAPKFVVWAVGPLMGPVTRDFIKRNVGLPLKFDNRRSRELGVKYRPA
                     EETFIEHFQQVIDDGLLKKK"
     gene            complement(1275..2276)
                     /locus_tag="PLY05_00525"
     CDS             complement(1275..2276)
                     /locus_tag="PLY05_00525"
                     /inference="COORDINATES: protein motif:HMM:NF027655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HNC01814.1"
                     /translation="MRKITVSLLSLAISHVVYAADIVIEEKSLSQPKTQAAPVTKPIQ
                     AQALSNEVNPSLQWQLYQQVQQLQQEVRDLRGQLEVQANIIERMKQDARSRYLDLDQR
                     ITDLKNRPQPEVANTTPSTTPTATTTTTTTTINAATATNPTTATPTTEATTATTPPVV
                     NPDDDKRAYFAAYQTFKTGGPNKAINPMRNFIKTYPQSTFIPSAYYWLGEFYLAASPA
                     DVNNAKKSFRIVVDNYADAPKAASAMLKLASFADVDGKTQDAVKLMLRIVKEFPKSEE
                     ATAARAYLSAQNVAIPEEKKAKPSTTKSELKDNKKVNETEKKPATSAKKEDSKTKTP"
     gene            complement(2285..3007)
                     /locus_tag="PLY05_00530"
     CDS             complement(2285..3007)
                     /locus_tag="PLY05_00530"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HNC01815.1"
                     /translation="MNNLIMVGHTDTGKTRTSNEDAILCLPNLGVALLADGMGGHSAG
                     EVASRTAIDTVSAILKQTTRGVSPHERLETALQAAHAVIREKARQSVRCRGMGTTFVG
                     ILIENGYLHHAHVGDSRLYLWRDGQLMAVTHDHSLLQEFIDQGFYTREEALEKVSRNI
                     LTRALGLEPHITIDYDYLKINKGDRFLLCSDGLYDMLSDYELGALLGREHDLEGIALD
                     MLELANARRSKDNVSVIVIAGF"
     gene            complement(3178..5748)
                     /locus_tag="PLY05_00535"
     CDS             complement(3178..5748)
                     /locus_tag="PLY05_00535"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNC01816.1"
                     /translation="MAKISFSWKSDWVIGLAIATFMAAISSSPIIENLERNAYDVGVK
                     SSQHNAGDKVAVIAIDDESIENIGRWPWPRSYLAQMIAGLNQGGAKAIGVPIFLSEPQ
                     IDAGLKSIRELNDYYNQNLSTLGDNNTVVAELGMKLAEAESNLNTDQQLADGMAAAKN
                     VVLPMQFMLGQMDGRPDTALPNYVLKNQIPVENIRDDIDATSLNMLPPETNLAVPPIS
                     VLGESASLLGHLSINPDSDGGVRTEPLLVSYDGAFYPSLSLMLAARSLNLDAKDIKVN
                     LGQSVQLGKINIKTDPFSQMRTFYYGNLEADSPPFRIDSFYDVVSGKIPVEKYKDKIV
                     IIGPTAYGLGSPQVTPIEAKMEPVLILAHNVSSILNQHFFTIPEWSSLVRWGLFLLAT
                     IYLCVLLPRLSAKLAAIVSVVFVGGLLMAHFALMISSGIWVPLMLPAALVVLGHVLLT
                     TKHYLVTEKAKVRSDEKSAESNKMLGLALQQQGQLDMAFEKFRQCPRDESILDVMYNL
                     ALDYERKRQFNKAISVYQYMADFAPEFRDLKDKLARAKKLEETVILGGSSSTANNSTL
                     ILDPTSNEKPMLGRYQVEKELGKGAMGVVYLGKDPKINRVVAIKTLALKEEFEADELE
                     EVKERFFREAETAGRLNHPNIVTIYDAGEEHDLAYIAMEFLKGHDLARYTKFDSLLPI
                     AKVINIVHKSALALDYAHQANIVHRDIKPANIMFDPDTSEVKITDFGIARITDSSKTK
                     TGTVLGTPTYMSPEQLAGKKVDGRSDLYSLGVMFYQMLTGTPPFKAESMAALMFKITN
                     EPHMPLFERRPDLESQIPCMSAVIDKALAKNADDRYQTGREFARAIKDCIESCGHKQV
                     "
     gene            complement(5891..6982)
                     /gene="ychF"
                     /locus_tag="PLY05_00540"
     CDS             complement(5891..6982)
                     /gene="ychF"
                     /locus_tag="PLY05_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNC01817.1"
                     /translation="MGFNCGIVGLPNVGKSTLFNALTKAGIAAENFPFCTIEPNTGIV
                     PMPDPRLDALADIVKPERVIPTSMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIAH
                     VVRCFEDENVIHVAGQINPLDDIATINTELALADLDTVERALLRVSKNAKGGDKEAIA
                     LKNCLEKVLPVLNDGRPARAADLSDDDLKVIKVYGLLTLKPTMYIANVNEDGFENNPH
                     LDAVRELAASEKAVVVPICNKLEAEIAELEEDEKAEFLEAVGMEEPGLNRVIRAGYNL
                     LGLHTYFTAGVKEVRAWTIPLGATAPQAAGKIHTDFEKGFIRAEVIAYEDYIHYKGEQ
                     GAKDAGKWRLEGKTYIMNDGDVVHFRFNV"
     gene            7062..7817
                     /locus_tag="PLY05_00545"
     CDS             7062..7817
                     /locus_tag="PLY05_00545"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HNC01818.1"
                     /translation="MNRVLYQFPLSHYCEKARWLLDFKDLEYSVKNLFPATHRLLTLW
                     KANSTTVPLVKDGKEWIGDSTELAFYLDAKYVLRPLLPSNPDLRRQAIILEDLADKAG
                     VHVRRWAYSQALESEQVTDLLFNDYPMVKPFKKQLAPFIKRGIGQMYQANPEKGAISL
                     QKMLKMMDDIEEKLIANGGRYLVGDCLGLADISVASLFVPVLALKDTPWETLTPQNEL
                     MEQLYQQTLNRPLGQWIIRLYQEERGARGNWRG"
     gene            complement(7862..10684)
                     /gene="rapA"
                     /locus_tag="PLY05_00550"
     CDS             complement(7862..10684)
                     /gene="rapA"
                     /locus_tag="PLY05_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HNC01819.1"
                     /translation="MSISNPAIGQRWLSDAETELGLGIVVEVNPRTMTILFPKSEETR
                     VYARQNAPLSRIRFNEGDTIKDTDNKSWLVTGIQERQFVLRYEVQDAAGNSTSFAETR
                     LSPEIHLAKPCERLLACQLDNNEWYELRVTALRAREKIAKSPVSGLVGPRVGLIPHQF
                     YIAHEVGQRMAPRVLLADEVGLGKTIEAGLIIHQQLLTGRAQRILVLVPDSLQYQWLV
                     EMRRRFNIDFALFDLTRTAAIREGDEEQNPFSTEQCVLASIDLLLDHPDLKESALAAK
                     WDLLVVDEAHHLEWDEEKPSEAYQLVEEIANQTAGVLLLTATPEQLGVASHFARLKLL
                     DPKRFSSLDNFLDEEEGYQPIAQAARDLVKGNLSAEAQQTLANYLADEDVDLNSKEGR
                     DQAVAALLDRHGTGRVLFRNTREAIKGFPQRECIPVPLTPPADWPMTGAVRHQLWPEM
                     QTDNEDWLTTDPRVPWLIQLLKKLKQQKVLLICRTAGVAMSLELRLRLNEGFRTALFH
                     EEMSLLERDRAAAYFAEPDYGAQILLCSEIGSEGRNFQFAHHLVLFDLPANPDVLEQR
                     IGRLDRIGQTETIKIHVPYMQGTAQERLFDWHHQALNTIAHISPTAQTVHQYHQATLK
                     DCLQNPTEHQQLFADLMAEAQQDRANLEAELQQGRDYLLELNSCRMDKAIDLITAVAD
                     QDDDLILGDFMERAWSAFGVDHEEQGDGSHIIRSGEHMPIDSFPCLDADGMTVCFDRN
                     QALSREDVQFITWEHPMALGTLDLMTRQAFGNANLGLIRNKGIKAGTLLIESWFRIEL
                     IAPKHLDLNTHLPQLTLRVLLDGEGRDLSSRIGHEMLNKQVQPLEKMTARQVVKMQTD
                     VMAALYQKTEKIAKAAIPELQQQAQDSLKAKLGQEIKRLKALQAVNPTIRDDEIARLE
                     QQLEQGLNYLSHVHVMSDALRIIIAA"
     gene            complement(10701..11330)
                     /locus_tag="PLY05_00555"
     CDS             complement(10701..11330)
                     /locus_tag="PLY05_00555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNC01820.1"
                     /translation="MSVDCPILFCDNDLLVVGKPSGLLTVRGKGADKQDCLHARLQAQ
                     FEEVRIIHRLDQDTSGLLVFARHLAAQQHLNKQFEQRLVNKHYIALVYGHIAQMRGEI
                     DAPLRYDPEHPPLHIVDYEQGQHALTQWQVLGRQAKATRVLLKPITGRSHQLRVHMQL
                     LGHPIVGDTLYAPPEAIALSPRLCLHAETLEFNHPITLQPMNFSWPCPF"
     gene            11488..12162
                     /locus_tag="PLY05_00560"
     CDS             11488..12162
                     /locus_tag="PLY05_00560"
                     /inference="COORDINATES: protein motif:HMM:NF027675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNC01821.1"
                     /translation="MLIYNDNHYHLNYYLSGVNLAKASLLKQLYQWHWISSAICLVGM
                     LVFSITGITLNHASQIEAKPKVERQELATPQQINQQLQTLAEQDQAQQPLPSNVEQWL
                     INQHGIHLSAESQVEWSADEVYISLPKAGGDAWARFSLANSQFEYESTSRGVISLLND
                     LHKGRHTGVVWQWFIDIFAIACVVFSLTGLFILQQHARRRPMVWPLVSTGLLIPLLLV
                     VFFVHI"
     gene            12175..12690
                     /locus_tag="PLY05_00565"
     CDS             12175..12690
                     /locus_tag="PLY05_00565"
                     /inference="COORDINATES: protein motif:HMM:NF021540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HNC01822.1"
                     /translation="MKKMIYGSALAMSLLSTAQAAELAVNVQIPSLNVAEYHRPYVAI
                     WIEDDSQKFMANLAVWYDLKKRDNGGTKWLKDLRQWWRKSGRELTMPVDGVSGATRNV
                     GEHQLSFSDKNTPLDKLAAGRYQLVVEAAREGGGREVLKVPFEWLSKKAHQNSAQGQE
                     ELGKVTVAVKP"
     gene            12712..13527
                     /locus_tag="PLY05_00570"
     CDS             12712..13527
                     /locus_tag="PLY05_00570"
                     /inference="COORDINATES: protein motif:HMM:NF022135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HNC01823.1"
                     /translation="MFKKTTLALGLLMLLPQAHAHRQWLLPSNTQIDAKEAWVTIDGA
                     VSENLFDFDSNALALDNLEVVAPDGSQVKAETITKSKFRGSADFKLLQKGTYKISQNS
                     QNVMATYRLNGEEKRWRGAEEKMAQEIPAEATDLKISRTYNRLETYVTADQPTDTVLK
                     AVNQGLELLPLSHPNNLYAQETTRFKVLLDGKPVADSKISVIAGGVRYRGVLNEITAI
                     SDKDGVISIKWPFAGMYWLKAAYPQLPEMVDGKRPPMPEKRYSYAATLEVLLP"
     gene            13681..>15722
                     /locus_tag="PLY05_00575"
     CDS             13681..>15722
                     /locus_tag="PLY05_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC01824.1"
                     /translation="MSIIRSRKKSTLLTSSMVAMAGSLAIPAQAQDAPVLKEVNVQAI
                     VDVPYKTEKSASSKLTQPLVDTPKTVQVLKKEMLKEQGASSLMEALRNTAGITMQLGE
                     NGNTAAGDTFQMRGFSTNTSTFVDGVRDLGAVTRDVFNLEQVEVVKGAGGSEVGRGAS
                     AGYINLISKLPTLEQANDIALTLGTADKKRLTVDSNNVIDDSTALRLNVMVQEANAEE
                     RNQVSNRQVAIAPSLAFGLDSPTKVYLYSQHIRQDNVPDGGVPTIGMEGFYNSNATIK
                     AGAKVSRDNFYGSQQDYEKISADMLTAKVEHQLSKNTTIRNISRYGQTHVDRVLTGVN
                     TLSVNASTKAIEVALNRQRIDQTNEIWANQTNLNTALSTGFIKHDLVLGLELLKESQL
                     TLGSTSGSTSTTTVVVNGQTLPVYSVVQNVYSPNAYQNLLVPYLTGADTDGETTTAAL
                     YVFDTLTLTDSLKFSLGLRADRYKTETSTGTMVTGGSGGNLATYSAQGYKVGDVVISH
                     LEDDDTLLSWNAGLVFKPAANGSIYVAFADAQTPPAGANFVLSATAGNQANAELDPQQ
                     TTTAELGTKWELLNKRLNVSAAVYRTENDKQASVDAVTGVANQEGKTRVDGIELAAVG
                     QLTNFWQLTMSVGFMESEQLSQSSKSSSTGVITTSDGVRWTPDVTASVWTSYTLEK"
     assembly_gap    15723..15822
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15823..16385
                     /locus_tag="PLY05_00580"
     CDS             <15823..16385
                     /locus_tag="PLY05_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HNC01825.1"
                     /translation="VKHNLQLSQNLPIAQQMAQQVREALLAHPLFVSAVLPKQLLLPL
                     FNCYQQGGHFGNHVDGAIQYEALTRFPVRTDVSTTVFLSEPDEYEGGELIIKDTYGSH
                     EVKLVAGDAIVYPATSLHRVEPVENGQRFAAFLWSQSMVKDNTQRQLLFDLDMTIIRL
                     RQQLQDSQDVLALTNHYHNLLRQWAE"
     gene            16382..17080
                     /locus_tag="PLY05_00585"
     CDS             16382..17080
                     /locus_tag="PLY05_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNC01826.1"
                     /translation="MMALSVSKDKLITLVVVLFVHSVALYWATRLNVLPPAPTPQKVV
                     MQALVIMPVALPTKPVEPLPQPTPPKSVVQPKEQPQPIVQKTPVLPPSEKAITLEKTV
                     MVEKNEPIVEKVSPTPVVNEPPVVSKVEAAPEPEPVVIPPRSDASGLNNTSPTYPAMS
                     RRLAEQGKVLLEVLILANGTVGEIKLKQSSGFKRLDDAALQTVKQWRFQPAKRGNQAI
                     DYWYIQPISFSLDT"
     gene            17096..17794
                     /locus_tag="PLY05_00590"
     CDS             17096..17794
                     /locus_tag="PLY05_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC01827.1"
                     /translation="MINDNPYGVMAIWEQGDIVMKSVALLLLAMSIISWYVIISRAWQ
                     VVKLRTIVKNSSAFWHSQSFEQGLELLEKNKANTFNPFVSLALNGQDAVEHHEQSKQD
                     LHGQISLTEWLKETLRADIEESAEKMQAGLPVLASVGSTAPFVGLFGTVWGIYHALVG
                     IGASGQASIDKVAGPVGEALIMTAFGLAVAIPAVLGYNTLTRSNKAIISKLQRFARQL
                     HAYFLTGSPRKGAV"
     gene            17796..18203
                     /gene="tolR"
                     /locus_tag="PLY05_00595"
     CDS             17796..18203
                     /gene="tolR"
                     /locus_tag="PLY05_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016141178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNC01828.1"
                     /translation="MSFNLDDDDEIMSEINMTPLVDVMLVLLIIFIITVPVLTHSVKV
                     DLPRANNQPNQIQNKPITISVNKQGQIYWNESLIDHTHLSQKLQQIAQQQPQPEVHIR
                     GDRKVEYEYVMQVMAEAQRNGVQKLGFITEPKS"
     gene            18333..>20366
                     /locus_tag="PLY05_00600"
     CDS             18333..>20366
                     /locus_tag="PLY05_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC01829.1"
                     /translation="MKLKSYWPMTLGLTIQPVLAEAVLPEVTVKGTIDVQEERRMASA
                     AKTIIDRKEIEATDASTVAELLNKLPMTGMFMEHPMGGVPKRGQGGRPQNRNMPQILV
                     DGQPLGGGSNMGTVMRLPVELIERVEIIRNSTPEFLVTSPAGVINIILKDVPKQLTRN
                     AKLTSFVADDRLGVRADGQYGQSQDNYGYLWAAAADSKPQIGERITTINDISQAQNSK
                     ETTQQTGRDNNLSVSPRFNLKLQNNQQIIISPFVAYNQDQRDAYTQRSFNIVNQELDS
                     TDGERLTGRLITEWKQQGQQNNEQSIKLFVQAEDENTDKTTYQTLQTANQLTYQENTD
                     RTERELALDGRIKSIFLETHIVSSGFEWRTKNTKEQLFKQQLLSNDSNIKEQRMALWW
                     QDEWLLSEKHTVTYGLRWQQFDNQINDTQQGFIEQKEDTFAPSIHYLWQPNEQWNIRA
                     SLAQHQRPARASELSSVIRTASGVNSSSNPDRAGNPQLANEKQYSVELGIEHYLPQKA
                     GNLSLSVFERQVSDYVQRLTLLENSRWLERPYNVGDARLRGILFDSKVQLNALELPNI
                     TLRSSYAYSQNTLTDAVANLGAGEGDRQTASLGIEYKFNNLTLGSSANYISAIDKESS
                     ATVRQEQGSQKSANAYVGYKINKQFNMRFSVNNLLAGDRTDTLYEYDAQGQLKR"
BASE COUNT         6100 a         3859 c         4288 g         6019 t
ORIGIN      
        1 aatcatgtat gggcagttta tcatatctaa tcaccgtaac ttttgttagc ctaaattgac
       61 aattatcaaa gtcatggcta atttgataga agctttttag tataaataga gttaatagca
      121 ctgaggtata aataatgatt gatgaaacga ttaaaaattt acctgtttta gtgacaggtg
      181 catcaggata tattgcaagt tggattgtaa aatacttgtt agaagaaggt tataccgttc
      241 atgctactgt acgtgatccg tcaaaacaat ccagtgttgg gcatttatta aaaattgcac
      301 aacaggctaa aggaacttta aagttattta aagcggattt attaactgaa ggaagttatg
      361 ccgaagcgat gcaaggttgt ggtgttgtta tgcataccgc atcacctttt gttttagatg
      421 gttttaccga tgccaatgaa gctttggttc gtcctgctct agaaggtaca cgaaatgttt
      481 taaattctgt taatcaatgt gaaacggtaa aacgtgttgt gttaaccagc agtgttgctg
      541 cgatatatgg cgataatgaa gatattttaa gagtccctag tggtattttt accgaagatc
      601 attggaatgt gaccagtagt gtttctcata atccgtatca atattctaaa gtagttgctg
      661 agcgtgaagc atggaaaatg caaggcaatc aatcacgttg ggatttagtg acaatcaatc
      721 cgagtatggt atttggccct tcattaacat caggtagtca atcagctagt attgatacct
      781 tagtgcaaat gggtgatggt cgtttgcgta tgggtgtacc taagctgatt ttagggattg
      841 tagatgtccg agaagtggca aaggcgcata ttttagcggc atttacgcca caagccaaag
      901 gacgacatat tttaagcaac accgaattga ccatgttaca aattggcaaa attttacgca
      961 aacaatatgg caacaaatat ccttttccat ttatggaagc accaaagttt gttgtatggg
     1021 cggtaggacc tttaatgggg ccagtgacac gtgactttat taaacgtaat gtcggtttgc
     1081 ctttaaagtt tgataatcgt cgtagccgag aattaggtgt taaatatcgg cctgctgaag
     1141 aaacctttat tgaacatttt cagcaagtga ttgatgatgg attgttgaag aaaaaataat
     1201 aataaatcta agcctacctc tttttataag aggtaggctt tttgcgtcct tgcttgaatt
     1261 aaaccctgtc aatcctaagg agttttagtt ttgctgtctt cttttttagc agaagtggca
     1321 ggttttttct ctgtctcatt tacctttttg ttgtctttaa gttcagattt agtggtgctt
     1381 ggtttggctt tcttttcttc aggaatagcg acattttgtg cgcttaaata agctctagct
     1441 gcggtcgctt cttctgattt gggaaactct ttaacaatcc tcaacataag tttaacagca
     1501 tcttgagttt tgccatcaac atctgcaaaa ctggccaact tcaacatggc agaagccgct
     1561 ttgggggcat cagcatagtt atcgacaaca attctaaagc ttttcttggc gttattgaca
     1621 tcggcagggc tagcagccaa ataaaactcg cctaaccaat aataggcact aggaataaac
     1681 gtgctttgtg ggtaagtttt aataaaatta cgcattggat taatggcttt attaggtcca
     1741 cccgttttaa acgtttgata ggcagcaaaa taagcgcgtt tatcgtcatc tggattgaca
     1801 acaggcggag tagtagctgt cgttgcctct gttgttggag tagcggtagt agggtttgtt
     1861 gcagttgctg cattaattgt tgtggttgtc gtggtagttg ttgctgtggg tgtggtgcta
     1921 ggtgtagtgt ttgccacctc tggttgaggg cgatttttta aatctgtaat acgttggtct
     1981 aaatcaaggt agcgactacg tgcatcttgt ttcatccgtt caataatatt agcttgcact
     2041 tcgagctggc cacgcaaatc tcgaacttct tgttgcaatt gttgaacttg ttgataaagc
     2101 tgccattgta atgaaggatt gacttcattg ctaagagctt gagcttgtat aggttttgtt
     2161 acaggtgcag cttgtgtttt gggttggctt aaagattttt cttcaatgac aatgtctgcc
     2221 gcataaacaa catgactaat cgctaaactg agtaaagaaa cggtgatttt gcgcataaat
     2281 taacctaaaa tcctgcaata acaataaccg acacattatc tttacttcga cgtgcgttgg
     2341 ctagctctag catatctaaa gcaatacctt ctaagtcatg ttcacggcct aataacgccc
     2401 ctaattcata atcagaaagc atgtcatata gtccatcact gcataataaa aagcgatcac
     2461 ctttattgat ttttaaataa tcatagtcaa tggtgatgtg aggctctaag ccaagagcgc
     2521 gagtaagaat attacgggaa accttttcta aggcttcttc acgagtataa aagccttgat
     2581 cgataaattc ttgcaataat gagtgatcat gggtaacggc cataagttga ccgtcacgcc
     2641 ataaatataa acgtgagtca cccacgtgag catgatgcaa atagccgttt tctatcagaa
     2701 tccccacaaa ggttgttccc atgccacgac agcgaaccga ttgacgtgct ttttcgcgga
     2761 taaccgcatg tgcagcttgt aaagccgttt ctaaacgttc gtgaggagat acaccacggg
     2821 ttgtttgttt aagaattgca ctaacggtat caatagcagt acgacttgca acttcacctg
     2881 cactatgtcc acccatacca tcagctaata atgcgacacc taaattgggt aggcaaagaa
     2941 tcgcatcttc gttgctagta cgtgttttgc cagtatccgt atggccaacc atgattaaat
     3001 tattcaaggt acaccagtta ttattttatc caatagttga gttaagttta tattatttgc
     3061 caaattaaag acttagacta aatcactaat taagaagtta ttctaaataa aatcaaggca
     3121 aataattaaa cctaaataac ctttttaggg tttagcaaag ctaaacccta tgaataatta
     3181 aacttgtttg tggccacaag actcaatgca gtctttaatt gcacgagcaa attcacgccc
     3241 tgtctgataa cgatcatcag catttttagc taatgcctta tcaatcactg cgctcataca
     3301 aggaatttga ctctctaggt ctggacgacg ctcaaataat ggcatgtgtg gctcattggt
     3361 gattttgaac attaaggcag ccatagattc tgctttaaat ggcggtgtac ctgttaacat
     3421 ttggtagaac atgacaccta agctatacaa gtcagagcga ccatcgactt ttttacctgc
     3481 taattgctct ggtgacatat aagtgggtgt acctaaaact gtacctgttt tagttttact
     3541 tgagtcagta atacgtgcaa taccaaagtc agtgattttg acttcgctgg tatctgggtc
     3601 aaacataatg ttggcaggct taatatcacg gtgaacaata ttagcttgat gggcataatc
     3661 taaagctaaa gcagatttat gaacaatatt aatgactttg gcgatgggta ataggctatc
     3721 gaatttagta taacgcgcta aatcatgacc ttttaaaaat tccattgcaa tataggctaa
     3781 atcatgttct tcgcccgcat cataaatagt gacgatattg ggatgattga gacgacctgc
     3841 ggtttcagct tcacggaaga aacgttcttt gacttcttca agctcgtcag cttcaaactc
     3901 ttctttgagt gctaaggttt taatggcaac cacacggtta attttggggt ctttacctaa
     3961 ataaacaaca cccattgcgc ctttaccgag ttctttttca acttgataac gacctaacat
     4021 cggtttttca ttactggtgg ggtctaaaat cagcgttgag ttattagctg tactgctgct
     4081 gccacccaag ataacggttt cttcgagttt tttggcacga gccaatttat cttttaaatc
     4141 tctaaattca ggcgcaaaat ctgccatata ttgataaaca gaaatcgctt tattaaattg
     4201 acgtttacgt tcgtaatcta aggctaagtt atacatcaca tctaaaatgc tttcatcacg
     4261 tggacattga cgaaactttt caaatgccat atctagctgg ccttgttgtt gtaaagccaa
     4321 gcccaacatt ttattagatt cagccgattt ttcatcagag cgtactttgg ctttttcggt
     4381 gactaagtaa tgtttggtgg ttaataaaac atgacccaag accacgagtg cagcaggtaa
     4441 catcaacgga acccaaatac cagagcttat cattaaggca aaatgtgcca ttaataatcc
     4501 acctacaaaa actactgaca caatagcagc aagttttgca cttaagcgag gtaataagac
     4561 acataaataa atagtggcta ataaaaataa accccaacgg actaagctgc tccattcagg
     4621 aatagtaaaa aaatgttgat ttaaaatgct gctaacattg tgtgccaaaa ttaatacagg
     4681 ctccattttt gcttcgatag gggtcacctg tggcgaacct aaaccataag ccgttgggcc
     4741 aataatcaca attttatctt tatatttttc gacaggaatt tttccgctga caacatcata
     4801 aaaagagtcg atacggaaag gagggctatc tgcttctaaa ttaccataat aaaaggtacg
     4861 catttgtgag aatgggtctg ttttgatgtt aattttaccc aattgcacac tttgtcctaa
     4921 gtttacttta atgtctttag catctaaatt taaacttctg gcagctaaca ttaatgataa
     4981 tgaaggataa aaagcaccat cataactcac taataatggc tcagtacgta caccaccatc
     5041 actatctggg ttaatgctta aatgacctaa taaactcgcg ctttcgccta atacactaat
     5101 gggcggaaca gctaagttgg tttcgggggg caacatattt agtgaggttg catcaatgtc
     5161 atcacgaata ttttcgacag gaatttgatt tttaagaaca taattaggca aagcagtatc
     5221 aggccgacca tccatttgac ccagcataaa ttgcataggt aataccacat ttttagccgc
     5281 tgccatacca tcggctaatt gttggtcggt atttaaattt gattctgctt cggcaagctt
     5341 catgcctaat tcagcaacaa cggtattgtt atcacctaag gtacttaaat tttgattgta
     5401 ataatcgttc agttcacgga tagattttaa accagcatca atttgcggtt cagataaaaa
     5461 aataggaact ccaatggctt tagcaccacc ttgatttaag ccagcaatca tttgggctaa
     5521 atagctacgt ggccacggcc aacgaccaat gttttcgata ctttcatcat caatcgcaat
     5581 gacagcaact ttgtcgcctg cattatgctg acttgatttg acgccaacgt cataagcatt
     5641 acgctcaaga ttttcaataa ttggcgagct agaaatagct gccataaaag tggcgattgc
     5701 taaaccaatg acccagtcgc ttttccaaga gaaagatatt tttgccatag tcgctaaccc
     5761 tgctgaatat atttattaag atttttgcgt attgtaagtg ctaaaaataa ttagtatttg
     5821 tatgcagtct ttgttgtttt ttgctccgtt taacggcaac acgccaaaac ggagcctagt
     5881 gaatcaaaaa ttacacatta aatctgaaat ggacgacatc accatcattc ataatatagg
     5941 ttttgccttc taaacgccat ttaccagcat ctttagcacc ttgctcacct ttataatgaa
     6001 tatagtcttc gtaggcaata acctctgcac gaataaatcc tttttcaaag tcagtatgaa
     6061 tcttaccagc cgcttgggga gcggttgctc ctaaaggaat tgtccatgca cgaacttctt
     6121 taacacctgc ggtaaaataa gtatgtaaac ctaataggtt gtaaccagca cgaatcacac
     6181 ggtttaaacc tggctcttcc atacccacag cttctaaaaa ttcggctttt tcgtcttctt
     6241 ccaattcggc aatttcggct tcaagtttgt tgcaaatagg aacaacgaca gctttttcgc
     6301 tggcggctaa ttcacgtact gcatctaagt gtgggttgtt ttcaaaacca tcttcattga
     6361 cgttggcaat atacatcgtt ggttttaaag tgagtaaacc ataaacttta atgactttta
     6421 gatcatcatc acttaaatcg gcggcacgag cagggcgacc atcatttaac acaggcaata
     6481 ctttttcgag gcagtttttt aacgcaattg cttctttatc accaccttta gcatttttgc
     6541 taacacgtaa caaagcacgt tcaactgtgt ctaaatctgc taatgctaat tcggtattaa
     6601 tcgtggcaat atcatctaaa ggattaattt gacctgcaac gtgaatcacg ttttcatctt
     6661 caaaacatct tactacatga gcaatcgcat cggtttcacg gatgtttgcc aaaaatttgt
     6721 tgcctaaacc ttcacctttg gaagcccctg caactaaacc tgcaatatca acaaactcca
     6781 tactggttgg aatcacacgt tcaggtttaa caatgtcggc taaagcatct aaacgaggat
     6841 caggcattgg cacaatacct gtattgggtt cgatagtgca aaaaggaaag ttttcggctg
     6901 caattcctgc tttagttaaa gcattaaata aggtagattt acccacattt ggtaagccaa
     6961 caataccgca attaaaaccc atttacaggc tcctcgtgtt aatttcgcaa tggcgcgcat
     7021 ggtacaccaa aatcctgttt tttgcttagg aatcgtatgg tatgaatcgt gtcctgtatc
     7081 aatttccctt gtcgcattat tgtgaaaaag cacgttggtt gttagatttt aaggatttgg
     7141 aatattcagt caaaaactta tttccagcca ctcatcgact tttaacctta tggaaagcca
     7201 atagcaccac tgtaccttta gtaaaagatg gcaaagaatg gatcggtgat tcaacagagt
     7261 tagcgtttta tttagatgct aaatatgtct tacgaccttt attaccgtct aatcctgatt
     7321 tgcgtagaca ggcgattatt ttagaggatt tagctgataa agcaggggtt catgtgcgtc
     7381 gttgggcata tagtcaggct ttagagtctg agcaagtcac agatttgtta tttaatgatt
     7441 atccaatggt taagcccttt aaaaaacaat tagccccttt tattaaacgt ggtattggtc
     7501 aaatgtatca agctaacccc gaaaaaggtg cgatttcttt acaaaaaatg cttaaaatga
     7561 tggatgatat tgaagaaaaa ttaatcgcta atggtggccg ttatttggtg ggtgattgtt
     7621 taggtttggc ggatattagt gtggcttcat tatttgtgcc agtattagct ctaaaagata
     7681 ccccgtggga gaccttgact cctcaaaatg aattgatgga gcaactttat cagcaaactt
     7741 taaatcgacc tttgggacag tggattattc gtttatatca agaagaacgt ggtgcaagag
     7801 gcaattggcg agggtgaaac aaaaaggcgc atacgcgcct ttttatgggt aactattgca
     7861 gttaagcggc aataataatg cgcaatgcat cactcatcac atgaacatgg cttaaataat
     7921 ttaaaccttg ctctaattgt tgttctaagc gtgcaatttc atcatcacga atggttgggt
     7981 taaccgcttg taaggctttt aggcgtttaa tttcctgacc cagtttggct tttaagctat
     8041 cttgagcttg ttgttgcaac tcaggaatag ccgctttagc aattttttcg gtcttctgat
     8101 aaagcgcagc catgacatca gtttgcattt ttactacttg acgtgcggtc attttttcta
     8161 aaggctgtac ttgcttatta agcatttcat ggccaatacg cgaactcaaa tcacgacctt
     8221 caccatcaag taatacgcgt aaagtcaatt gtggcaaatg tgtgtttaaa tctaaatgtt
     8281 taggtgcaat taactcaatt ctaaaccaag attcaatcaa caaagtaccc gctttgatgc
     8341 ctttgttacg aatcaggcca aggttagcat taccaaaggc ttgacgtgtc attaggtcta
     8401 aagtgcctaa tgccattggg tgttcccacg taataaactg cacatcttca cggcttaagg
     8461 cttggttacg gtcaaaacaa acggtcatgc catcggcatc taaacaaggg aaggagtcaa
     8521 ttggcatatg ttcgccacta cgaataatat gactaccatc accttgctct tcatggtcaa
     8581 caccaaaagc tgaccatgcg cgctccataa aatcacccaa gattaaatca tcatcttggt
     8641 cagcaacggc agtaatcaag tcaatcgctt tgtccatacg gcacgaatta agctctaaca
     8701 aataatcacg accttgttgt aactctgctt ctaagttggc gcgatcttgt tgtgcttcag
     8761 ccattaaatc ggcaaataat tgttgatgtt cggttggatt ttgcaaacaa tcttttaaag
     8821 tagcctgatg atattggtgt acagtttgag ctgttggcga aatatgagca atggtattta
     8881 aggcttggtg atgccagtca aataaacgct cttgggcagt gccttgcata tagggcacat
     8941 gaattttgat ggtttcagtt tgaccaatac gatccaaacg gccaatacgt tgctctaaaa
     9001 catcaggatt agcgggtaag tcaaacaaca ctaaatgatg ggcaaattgg aagttacgac
     9061 cttcagagcc aatttctgaa cataacaaaa tttgtgcacc atagtcaggt tcagcaaaat
     9121 aggctgctgc acggtcacgt tctaatagac tcatttcttc atggaaaagt gctgtgcgga
     9181 aaccttcatt taggcgtaaa cgcaactcta aagacatggc cacgcctgcg gtacggcaga
     9241 ttaacaacac tttttgctgt ttgagctttt tcaataattg aatcaaccac ggcacacgtg
     9301 ggtctgtggt taaccaatct tcattatcgg tttgcatttc tggccagagc tgatgacgca
     9361 cagcacctgt cattggccaa tcagcagggg gtgttaatgg cacaggaata cattcgcgtt
     9421 gtggaaagcc tttaatggct tcacgcgtat ttctaaataa aacacgacct gtgccatgac
     9481 gatctaataa tgcagcaaca gcctgatctc gaccttcttt gctgtttaag tctacgtctt
     9541 catcggccaa gtaatttgct aaagtttgtt gtgcttcagc acttaaatta cctttgacta
     9601 agtcacgcgc agcttgtgca atcggttgat aaccttcttc ttcatctaag aagttatcta
     9661 ggctgctaaa acgttttggg tctaaaagtt ttaaacgggc aaagtggcta gcaacaccca
     9721 attgttctgg ggtggcggtg agcaataata ccccagccgt ttggttggca atttcctcaa
     9781 ccaattggta ggcttcgctc ggtttttctt cgtcccattc taaatggtgt gcttcgtcaa
     9841 ccactaataa atcccattta gcggctaaag cagactcttt taaatcggga tgatcgagta
     9901 ataagtcaat gctggctaaa acacattgtt cggtactaaa cggattttgt tcttcatcac
     9961 cttcacgaat cgcagcggtg cgggttaaat caaataaggc aaagtcgata ttaaaacgac
    10021 gacgcatttc aaccaaccat tgatactgca aactatcagg gactaacacc aaaatacgtt
    10081 gtgcacgacc tgttaacaat tgttgatgaa taattaaacc tgcttcaatc gtttttccca
    10141 aacccacttc gtcagctaat aacacccgtg gtgccatacg ttggccaact tcgtgagcaa
    10201 tataaaattg atgcggaatt aaacccacac gcgggccaac caaaccagat actggacttt
    10261 tggcaatttt ttcacgcgca cgtaaggcag tgacacgcaa ttcataccat tcattattat
    10321 ctaattggca ggccaaaaga cgttcacaag gtttggctaa atgaatttcg ggagataagc
    10381 gagtttcagc aaagctggtg gagttgccag cagcatcttg tacttcataa cgcaagacaa
    10441 attggcgttc ttgaatgcct gttactagcc atgatttatt gtcagtatct ttaatggtat
    10501 cgccttcatt aaaacgaatc cgtgataagg gcgcattttg gcgcgcatag acacgggttt
    10561 cttcgctctt gggaaataag atggtcatgg tgcgaggatt tacctcaacc acgataccta
    10621 agccaagctc agtttcggca tcagataacc aacgttgacc aatagcagga tttgaaatac
    10681 tcatgtaaca gtcctaacaa ttaaaaaggg catggccatg aaaaattcat tggctgtaaa
    10741 gttatgggat gattaaattc tagtgtctct gcgtgtaaac acaaacgcgg acttaaagca
    10801 attgcttcgg gtggtgcata taatgtgtca ccaacaatcg gatgacctaa taattgcata
    10861 tgcacacgca attgatgaga gcgaccagta attggtttta acaacacacg ggtggctttg
    10921 gcttgacggc caagcacttg ccattgggtg agggcgtgtt ggccttgctc ataatcgaca
    10981 atatgtaaag gcggatgctc agggtcataa cgtaatggtg catctatttc gccacgcatt
    11041 tgagcaatat gaccatagac taaggcaata tagtgtttgt taaccaaacg ttgttcaaat
    11101 tgtttattga ggtgttgttg tgctgccaaa tgacgagcga agactaatag accagaagta
    11161 tcttggtcta gacgatgaat aatacggact tcctcaaatt gggcttgcaa acgagcatgt
    11221 aaacagtctt gtttgtcagc acctttgcca cgaacagtta ataaaccact gggtttaccc
    11281 accacgagca aatcattgtc acaaaataaa atggggcaat ctacagacac gcgcagtttt
    11341 cttttaagtc atgaaagatg ctaattgtaa taatagcgac aaaggattgc atctaattat
    11401 ctctaaagca tgataaataa aaccattttt gtgtattttt tgaccttatt ggaaaattga
    11461 aaagtatttt ttacacaaag ttagcatttg ttgatatata atgataatca ttatcattta
    11521 aattattatt tatcgggtgt gaacttggct aaggcatcgt tgttaaaaca gttgtatcag
    11581 tggcattgga ttagctcggc gatatgtttg gtagggatgt tggtgtttag tattacaggc
    11641 attactttaa atcatgctag ccaaattgag gcgaaaccca aagttgagcg tcaagaactg
    11701 gcaactcccc agcaaataaa ccaacaattg caaacgcttg cagagcaaga tcaagctcaa
    11761 cagcctttac caagcaatgt tgagcaatgg ttgattaatc aacatggcat tcatttaagt
    11821 gccgaatcac aagtcgaatg gtctgctgac gaagtttata tttcgttgcc caaagcgggt
    11881 ggtgatgcgt gggcgcgctt ttccttagcc aatagtcagt ttgagtatga atctaccagt
    11941 cgtggtgtga tttccttatt aaacgacttg cataaaggtc gtcatacggg cgtggtttgg
    12001 caatggttta ttgatatttt tgccattgcc tgtgtggttt tttccttaac gggactgttt
    12061 attttgcaac agcatgcccg tcgtcggcca atggtttggc cactcgtcag cacaggattg
    12121 cttattccct tattgttagt ggtgttcttt gttcatatct aaagagagat atttatgaaa
    12181 aaaatgatct atggttcggc cttagccatg agcttgctat cgacggcaca agctgctgag
    12241 ttagccgtta atgtgcaaat acccagttta aatgtggccg aatatcatcg tccttatgtt
    12301 gccatttgga ttgaagatga tagccaaaaa tttatggcta atttggcggt ttggtatgac
    12361 ctaaaaaaac gcgataacgg tggcacaaaa tggttaaaag acctgcgtca atggtggcga
    12421 aaaagtggtc gtgaattaac gatgcctgtt gatggtgtat ctggtgcaac acgtaatgtc
    12481 ggtgagcatc aattaagctt tagcgataaa aatacgccat tagataaatt ggccgcagga
    12541 cgttatcagt tggtcgttga ggcagcacgc gaaggcggtg gtcgagaagt gttaaaagtg
    12601 ccgtttgagt ggttgtctaa aaaagcccat cagaatagtg cacaaggaca agaagaatta
    12661 ggcaaagtca ccgtcgcggt taaaccgtaa tcattaaaac aggacaatat catgtttaaa
    12721 aagacaacgc ttgctttggg cttattaatg ttgctcccgc aagctcatgc acatcgtcaa
    12781 tggttattac cctccaatac acagattgat gctaaagaag catgggtgac tattgatggg
    12841 gcggtatcag aaaacttatt tgattttgat agtaatgctt tggctttaga taatttggag
    12901 gttgttgccc ctgatggtag tcaggtgaag gcggaaacaa tcactaaaag caaatttcgt
    12961 ggctctgctg attttaaatt gctacaaaaa ggcacttata aaattagcca gaactcacaa
    13021 aatgtgatgg caacttatcg tttaaacggt gaggagaaac gttggcgtgg ggcagaagaa
    13081 aagatggcac aagaaattcc agccgaagcc accgatctaa aaatatcgcg tacctataac
    13141 cgtttagaaa cctatgtcac agcagatcaa ccgactgata cggtgctaaa agccgttaat
    13201 caaggtttag agttattgcc gttaagccat cccaataatt tgtatgcaca agaaacgact
    13261 cgttttaaag tgttattaga tggcaagcct gttgcggata gcaaaatatc agtgattgca
    13321 ggcggtgttc gttatcgtgg tgtattaaat gaaatcacag caatcagtga taaagacggc
    13381 gtgattagca ttaaatggcc atttgcagga atgtattggt taaaagcggc ttatccacaa
    13441 ttgccagaaa tggttgatgg caaacgccca cccatgcccg aaaaacgtta tagctatgcg
    13501 gcaaccttag aggtgttatt accgtaattg ttgcaagttg ttgcaaattt aaatgataat
    13561 tgttatcatt tacaaagtgc ccagccagtt ctaaacgaca agccaagcct tttttctgtt
    13621 taacccatag atgggatgtt cattgcatct aactcttagg gctatttgag gtattgtgtt
    13681 gtgtctatca ttcgttctcg taaaaaatct accttattaa catcctctat ggtggcgatg
    13741 gctgggagtt tggcgattcc tgcgcaagcg caagatgcac ctgtgttgaa agaagttaat
    13801 gtacaggcca ttgttgatgt gccttataaa accgaaaaat ccgcttcctc taaattaacg
    13861 caacccttgg tagatacccc aaaaaccgta caagtgctaa aaaaggaaat gctgaaagag
    13921 caaggtgcta gctccttgat ggaagcctta cgcaataccg caggcattac tatgcagttg
    13981 ggcgaaaatg gtaatacggc ggcaggtgat acgtttcaga tgcgtggttt ttcaaccaat
    14041 acctcaactt ttgttgatgg tgtccgtgat ttaggtgcag tgacccgtga tgtatttaac
    14101 cttgagcaag ttgaagtggt taaaggcgct ggtggttcag aggtgggacg tggtgcgagc
    14161 gcgggttata ttaatttaat ttccaaattg cctactcttg agcaggccaa tgatattgca
    14221 ttaacactgg gcacagccga taaaaagcgt ttaactgttg atagcaataa tgtgattgat
    14281 gacagcacag cattacgttt gaatgtcatg gtgcaagaag cgaatgccga agagcgtaat
    14341 caagtttcca atcgacaagt ggcaattgca ccaagtttag cctttggctt agattcacca
    14401 accaaagtat atctctattc tcagcatatt cgtcaggaca atgtgcccga tggtggtgta
    14461 ccaactattg gcatggaagg tttttataat agcaatgcca ctattaaagc aggggcaaaa
    14521 gtcagtcgtg ataactttta tggcagtcag caagattatg aaaaaatatc tgcagatatg
    14581 ttaacggcca aagtggaaca tcagttatca aaaaacacca ccattcgtaa tattagccgt
    14641 tatggtcaaa cacacgtaga tcgtgtgtta acgggggtga atactttatc tgtcaatgcc
    14701 agcactaaag cgattgaagt cgctttgaat cgccaacgta ttgaccaaac taatgaaatt
    14761 tgggcaaacc aaacaaactt gaataccgcg ttaagcacgg gttttattaa acatgattta
    14821 gtgcttggtt tagagttatt aaaagaaagt cagttaacac ttggctcaac ttcaggcagc
    14881 actagcacaa caacggtggt ggtgaatggc caaactttgc cagtatatag tgttgtacaa
    14941 aatgtatata gcccaaatgc ctatcagaat ttgttagtgc cttatttaac gggtgcggat
    15001 acagacggtg aaacgacaac agccgcattg tatgtctttg acactttaac attaacagac
    15061 agtttaaaat ttagccttgg tttacgtgcc gatcgttata aaactgaaac tagcacaggt
    15121 acgatggtaa cgggtggtag tggtggcaat ttggctacct attccgcaca aggttacaaa
    15181 gtcggtgatg tggttattag tcatttagaa gatgacgata ccttattaag ctggaatgct
    15241 ggtttagtgt ttaagccagc tgcgaatggt tcgatttatg ttgcctttgc cgatgcacaa
    15301 acaccacctg cgggtgctaa ctttgtgtta tcagcgaccg caggtaatca ggcaaatgca
    15361 gaattagatc cacaacaaac cacaaccgcc gaattgggta caaagtggga gttgttaaat
    15421 aagcgtttaa atgtctctgc tgctgtttac cgtaccgaaa acgataaaca agccagtgtt
    15481 gatgcggtga ctggtgtggc taatcaggaa ggaaaaaccc gtgttgacgg tattgaatta
    15541 gcggcagttg gtcaattaac gaacttttgg caattaacca tgtctgttgg ttttatggaa
    15601 agtgagcagt taagccaaag ttctaagagt agttcaacgg gtgtgattac cacatcagat
    15661 ggtgtgcgtt ggacacctga tgtcaccgca tcggtatgga catcttatac cttagaaaaa
    15721 ttnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaggtcaaa cataatttac
    15841 aactctcaca aaacctacct attgctcagc aaatggcaca gcaagtgcga gaggctttat
    15901 tagctcatcc cttatttgtc tctgccgtat tgcccaaaca attgttgttg cccttattta
    15961 attgttatca gcagggagga cattttggta atcatgtcga tggcgcgatt caatacgaag
    16021 ctttaacacg ttttcctgtt cgtaccgatg tctcaaccac tgttttttta tcagagcctg
    16081 atgagtacga aggtggcgag ttgattatta aagatacata tggcagtcat gaggttaagt
    16141 tggttgcagg tgatgcgatt gtttaccctg cgaccagttt acatagggtt gagcctgtag
    16201 aaaatggtca acgttttgca gcgtttttat ggtcacaaag tatggtcaaa gataatacgc
    16261 aacgacaact gttatttgat ttggatatga ctattattcg tttgcgtcag caattacaag
    16321 acagccaaga tgttttggca ttaaccaatc actatcataa tttattaagg caatgggctg
    16381 aatgatggca ttatcggttt ctaaagataa gctaattact ttagtggtgg tgctatttgt
    16441 acacagtgtg gctttatatt gggcgacacg actaaatgta ttgccgcctg cacctactcc
    16501 ccaaaaagta gtcatgcaag cactagtgat tatgcctgtt gccttgccga ctaaacccgt
    16561 agagccttta cctcaaccta caccgcctaa atctgtggtt caacctaaag agcagccaca
    16621 gccgattgta caaaaaacgc ctgttttacc gccctccgaa aaagccatca cccttgaaaa
    16681 aacagttatg gtcgaaaaaa acgaacccat tgttgagaaa gttagtccaa cgcctgttgt
    16741 taatgaacca cctgttgtga gtaaagttga ggctgcgcct gagcctgagc cagtcgtgat
    16801 tccgccacgc agtgatgcga gtggtttaaa taataccagt ccgacatatc ctgccatgtc
    16861 tagacgtttg gccgaacaag gcaaagtgtt attagaggtg ctgattttgg ctaatggtac
    16921 agtgggcgag attaagctta aacaaagcag tggttttaaa cgtttagacg atgctgcctt
    16981 acaaaccgta aaacaatggc ggtttcaacc tgccaagcgt ggtaatcagg cgattgatta
    17041 ttggtatata cagcctattt ctttttcttt agatacctaa tttagagttt atattatgat
    17101 taatgacaat ccttatggcg taatggcaat ttgggagcaa ggcgatatag tgatgaaatc
    17161 agtcgcctta ttgttgttgg ctatgtcgat tatttcttgg tatgtgatta ttagtcgagc
    17221 atggcaagtc gttaaactca gaaccattgt caaaaatagt agtgcttttt ggcatagtca
    17281 aagttttgag caaggcttag agttgttaga aaaaaataaa gcaaatacgt ttaatccttt
    17341 tgtgagtttg gctttaaatg gccaagatgc cgttgaacat catgagcaaa gtaaacaaga
    17401 tttgcatggc caaattagtt taaccgaatg gttaaaagaa actttgcggg cagatataga
    17461 agaaagtgcc gaaaaaatgc aggcgggatt acccgtttta gcttcagtgg gttcgactgc
    17521 gccgtttgtg ggattatttg ggacggtttg gggaatttat catgccttag taggcattgg
    17581 cgcatctggc caagcaagta ttgacaaagt ggcagggcct gttggcgaag cattgattat
    17641 gactgctttt ggcttggcgg tggctattcc agcagtgtta ggctacaaca ccttaacacg
    17701 ctctaataaa gcgattatca gtaaattaca acgttttgct cgtcaattac atgcttactt
    17761 tttaacaggc tcaccacgta agggggcggt ttaagatgtc gtttaaccta gatgatgacg
    17821 atgagatcat gagtgaaatc aatatgacgc ccttggtgga tgtcatgttg gtcttattaa
    17881 ttatttttat tattactgtg cctgtattga cgcattcagt aaaagtggat ttgcctaggg
    17941 caaacaatca gcctaatcaa atacaaaata agccaattac tatttcggtt aataaacaag
    18001 gccaaattta ttggaatgag agtttgatag atcacactca tctcagtcaa aaattgcaac
    18061 aaattgctca acaacaacca caaccagaag ttcatattcg tggtgacaga aaggttgagt
    18121 atgaatatgt aatgcaagtg atggcagaag cccaacgcaa tggcgtgcaa aagttaggtt
    18181 ttattactga accaaaatct taatgatttc gactacgctc aattatctca actctgtacc
    18241 ctgagcgcag ttccgcggtt agacttggct aaccatacgc aaaaaataca gataaatttt
    18301 ggttttagtg ctttgttttt tgagaaaaat ttatgaaatt aaaatcttat tggcctatga
    18361 ccttaggttt aaccatacaa cctgtgttgg ccgaagcggt attacccgaa gtgacagtaa
    18421 agggaactat agatgttcaa gaagagcgaa ggatggcgtc agctgctaaa acaattattg
    18481 atcgtaagga aatcgaagca acagatgcaa gtacggttgc tgaattatta aataaattac
    18541 ccatgacagg tatgtttatg gaacatccta tgggtggtgt gccaaaacgt ggtcaaggtg
    18601 ggcgcccaca aaatcgaaat atgccacaaa ttttagtcga tggtcagcct ttaggtggcg
    18661 gtagtaatat gggaacggtg atgcgtttgc ctgtagagct cattgaacga gtggagatta
    18721 ttcgcaatag tacgcctgag tttttggtca ccagccctgc tggagtcatt aatattattt
    18781 tgaaggatgt tcccaaacaa ctaacgcgaa atgcaaaact aacaagcttt gttgctgatg
    18841 acagattggg agtacgtgct gatggacaat atggtcaaag ccaagataac tatggctatt
    18901 tatgggcggc agcagcagat agcaaaccac aaattggcga acggataacc acaattaacg
    18961 atattagcca agcacaaaat agcaaagaga caacacaaca aacaggacgt gataataatt
    19021 tatctgtgtc acctagattt aatttgaagc tacaaaataa tcaacaaatt attatcagtc
    19081 cttttgttgc ctataaccaa gatcagcgcg atgcctacac tcaacgtagt tttaatattg
    19141 tcaatcaaga gttagacagc actgatggcg agcgtttaac agggcgttta atcaccgaat
    19201 ggaaacaaca aggccaacaa aataatgagc aaagtattaa gttatttgtt caagctgaag
    19261 atgaaaatac cgataaaact acttatcaaa cattacaaac agcgaatcaa ttaacatatc
    19321 aagaaaatac cgatcgtact gaaagagagc ttgctttgga tggccgaatc aaaagtattt
    19381 ttttagaaac acatattgtt agctctggtt ttgaatggcg caccaaaaac actaaagagc
    19441 aattatttaa acagcaatta ttaagcaatg attccaacat taaagaacaa cgcatggcat
    19501 tatggtggca agatgaatgg ttgctgtcag aaaaacatac cgtaacttat ggcctacgtt
    19561 ggcaacagtt tgataatcaa attaatgata cacaacaagg ctttattgaa caaaaagaag
    19621 acacatttgc gccttctatt cattatttat ggcaacccaa tgagcagtgg aatattagag
    19681 ctagtttagc tcaacatcag cgtcctgcac gtgcgagtga gctaagttcc gtgattcgta
    19741 ccgcatcagg tgttaatagc tctagtaatc ctgatcgggc aggtaatcca caattagcca
    19801 atgaaaagca atatagtgtt gaattgggta ttgaacatta tttgccacaa aaagcaggca
    19861 atttgtcttt atcggtgttt gaacgtcaag tgagtgatta cgtgcagcgt ttgacattgc
    19921 tcgaaaatag tcgttggttg gagcgacctt ataatgtcgg tgatgcacgt ttgcgtggca
    19981 tattatttga tagcaaagtg caacttaatg ctttagagct acctaatatc accttacgga
    20041 gtagttatgc ctatagtcaa aataccttaa cggatgctgt ggctaattta ggagctggtg
    20101 aaggagatcg tcaaacggca agtttgggca ttgagtacaa atttaataat ctgacattgg
    20161 gttcatctgc gaattatatt tctgcgattg ataaagaaag tagtgcaacg gttcgccaag
    20221 agcaaggttc gcaaaaatca gccaatgctt atgtgggtta taagataaat aagcaattta
    20281 atatgcgttt ttcggtgaat aatttattgg caggcgatag aacagacaca ttatatgagt
    20341 atgatgcaca aggccaatta aaacgt
//
LOCUS       DAOQMB010000006        19665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_964_length_19665_cov_15.822030, whole genome shotgun sequence.
ACCESSION   DAOQMB010000006 DAOQMB010000000
VERSION     DAOQMB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19665
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_19665_cov_15.822030"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..2065)
                     /locus_tag="PLY05_00605"
     CDS             complement(137..2065)
                     /locus_tag="PLY05_00605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNC01830.1"
                     /translation="MFDFFGRQQQARSATRYLLIYLVLVMVLIAVAVNIFFYFVAILT
                     GYDDGQGSWLWHVWSAKAYGATVSLIASGSMVEWFLLREGGVALANMIQANKIDFASK
                     DSQQQQLVNVCEEMAIAAGMTMPSLYVLHQEKTINAFVAGYNLQDSILVVTQGTLEHL
                     NRDELQAIIGHEYSHIINGDMRLNTYLVSILAGIVNIGQAGDYLIRLSYLGSQKQIKP
                     KKIWPMPIIYVVGLIIWLIGYIGLFFARLVKAAISRSRENLADAASVQFTRYPDALAS
                     ALYKISQHGSLLQNRYAEQLSHMCFADSVLFAPYLASHPPIKQRINIISPSFLTRMKY
                     RQDPIKVEEPQPKTSASIPVSIQADGLIAFSAVELPIIAATMTASKVGDLQWQDLMSA
                     QYLHRQLAVEVSRALQGTQGAKAVIFALVANQQHTPTQFIRDFFNQQPAFAQTIIQLQ
                     QHLTTVDTRLALPVVELAIPRLQRLTEQEQQSFISELKRFVGQIDNVFEFALLSLIEQ
                     QLSKTKTILRQLPLTRLAQPCAELIATLLHYGAHPIHLQKQIYQQLMPTMFAEHTPVM
                     PVIEKDNFTVLAKALQQLQYLTPKAKQQLITLAAATIQSDGILHRAEYELLRVLASLL
                     ACPMPLLLGQIHVDRNPQ"
     gene            complement(2072..2662)
                     /locus_tag="PLY05_00610"
     CDS             complement(2072..2662)
                     /locus_tag="PLY05_00610"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HNC01831.1"
                     /translation="MMSLFFWAMLILAIVYGINAYNGLISLRNQFKNAFAQIDVQLQR
                     RYELIPNLVEAVKAYLSHEKDTLTQVINARNQAITVEQMAAKNSDNQYAINQLGEAEG
                     VLNQALGRLMAISEAYPDIKANSTIQNLMEELKSTENRVSFARQAFNDAVMIYNTERE
                     KFPNNLVANMTGFQEATLLKISHSEAKEPVKISFNG"
     assembly_gap    2756..2855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2939..>4239
                     /gene="rimO"
                     /locus_tag="PLY05_00615"
     CDS             2939..>4239
                     /gene="rimO"
                     /locus_tag="PLY05_00615"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNC01832.1"
                     /translation="MSVQQPKVGFVSLGCPKNLVDSERILTQLRAEGYQISPDYNGAD
                     LVVVNTCGFIESAVQESLDAIGEALAENGKVIVTGCLGIKEGQIRDVHPSVLKVTGPH
                     AYEEVMDSVHQYLPPKKDHNPFIDLVPAQGIKLTPKHYAYVKISEGCNHRCTFCIIPS
                     MRGDLVSRPIGSVMAEAQNLVKSGVKELLVISQDTSAYGVDLKYKLDFWNGQPLKTRM
                     QELCEAMSTLGAWVRLHYVYPYPHVDNVIPLMAEGKILPYLDIPFQHASPRILKLMKR
                     PAHSENTLERIKQWRKICPDLVLRSTFIVGFPSETEEEFEELLNWLREAQLDRVGCFK
                     YSPVDGAAANELPAPIPEDVKEARWQRFMEVQQEISAQRLQLKIGKTIQVLIDDIDEE
                     EGVAIARSAADAPEIDGNVYIEGEGAADLKIGDFATVTIIDA"
     assembly_gap    4240..4339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4357..5757
                     /locus_tag="PLY05_00620"
     CDS             4357..5757
                     /locus_tag="PLY05_00620"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC01833.1"
                     /translation="MRKLRFLGAAQEVTGSCYLLENAGHHILLDCGIHQGGDGVDRIT
                     KEEFKFAPASIDAVVLSHAHLDHSGLLPKLVHEGFKGKIYCTHGTHKLLKILLEDAVH
                     IYLKDLEYENLRRERAGKKPLFPQYTLDDVQTVLNLCCALDYHQAHTFLEKFTLTFYD
                     AGHILGSSIVQLVLNDNHNAKTLVFSGDLGNPDTSLMRNFDFIDNADVVLMESTYGDR
                     DHRCFAETLDEFKAILKQAEQDGGNILIPAFAVGRTQELLFHLGKMYHEGLLDHWHIF
                     LDSPMAKAVTNVYSDCIKQLDKEDVAFITAHQGEDISSFLPNLTISESVEDSAAINNI
                     KKGAIIIAGSGMCTGGRIRHHFKHRLWHTNTHVVFVGFQARGTLGRILVDGVKHVKLF
                     GLPILVKAQIHTLGGFSAHAGQKELLAWAKNFKTQPRFYLVHGEAKTMEKLSEKLAQE
                     LGINAEIPAQLSQIMF"
     gene            complement(5793..6506)
                     /locus_tag="PLY05_00625"
     CDS             complement(5793..6506)
                     /locus_tag="PLY05_00625"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNC01834.1"
                     /translation="MKQAIFCWSGGKDSAYALHQILQAGEYEVKYLLTTINGEFQRIS
                     MHGVRELLLEQQASAIGIPLLKVKVYQGSNSEYEQQMAQTLLQAKAEGISHVIFGDIF
                     LADLRAYREKNLAQVGLEAVFPLWQKNTTQLINDFIAQGFKTITCCVSDAYLNEQWVG
                     REINQQFVAELPAQVDACGENGEYHTFCYDAPYFKHAVAFELGEKVYKPLEIKSTDAV
                     CSSDNQTQGFWFCDLIAVN"
     gene            complement(6503..6865)
                     /locus_tag="PLY05_00630"
     CDS             complement(6503..6865)
                     /locus_tag="PLY05_00630"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HNC01835.1"
                     /translation="MQKKCSVCGVAFACGNTGQQACWCNQLPAIMPLDFEQDCRCPQC
                     LKVVIKQGIEQFVQQNPVTVAIPKQYHNAQLIEDIDYYLEDGKWVFTEWYHRKRGSCC
                     GNGCRHCPYQHINVKSKL"
     gene            complement(6937..8358)
                     /locus_tag="PLY05_00635"
     CDS             complement(6937..8358)
                     /locus_tag="PLY05_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC01836.1"
                     /translation="MAERLNNDFQFLDVARHDPAKKDIAVRKSEYVEIYHSFTKVEVG
                     EQAHRCLACGNPYCEWKCPVHNYIPNWLKLISEGNIFGAAELCHQTNTLPEVCGRVCP
                     QDRLCEGACTLNDGFGAVTIGNAEKYIVDTAFAMGWRPDMSKVKWTNKRVAIIGAGPA
                     GLGCADVLVRSGVKPVVFDKNPEIGGLLTFGIPEFKMEKSVMSRRRTIFTDMGIEFRL
                     NTEVGKDVQFTDLLNEFDAVFLGMGTYTYMKGGFAGEDLPNVYDALDFLIANVNHNNG
                     WEKDANDFISMKGKKVVVLGGGDTAMDCNRTSIRQGADAVTCAYRRDEENMPGSRREV
                     NNAREEGVNFLFNRQPIAILGDENGVTGVKVVETRLGSPDARGRRSPEPIPNSEQVLP
                     ADAVILAFGFRPSPAPWFADFGINLDNSGRVVAPAESEFKYQTSNPKVFAGGDMVRGS
                     DLVVTAIWEGRQAAEGILDYLNV"
     gene            complement(8468..>12352)
                     /gene="gltB"
                     /locus_tag="PLY05_00640"
     CDS             complement(8468..>12352)
                     /gene="gltB"
                     /locus_tag="PLY05_00640"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HNC01837.1"
                     /translation="YKGLVMPADLPSFYKDLQDERLASHIVVFHQRFSTNTLPKWPLA
                     QPFRYLAHNGEINTIMGNRNWSVARTPKFESPLLPGLLDCKPLVNRTGSDSSSLDNML
                     EILVGGGMNLFRALRMLVPPAWQNVETMDADLRAFYEYNSKHMEAWDGPAGLVIQDGR
                     HAICMLDRNGLRPARWVITKNGYITLASEIGVWDYQPDDVVSKGRVGPGQILVVDTQT
                     GELLRTADIDQRLKSAHPYRTWLRDQAIRLENDDVVDKRGLGQMDAAQLKTYQKMFLV
                     TFEERDQIIRPLAESGQEAVGSMGDDTPMAVLSRRVRHMADYFRQQFAQVTNPPIDPL
                     REAIVMSLETCLGAEMNVFEETAEHAHRAILASPVLSFSKFQQILALNDQKGYANAII
                     DLNYAESEGLQAAIARICDEAETAVKNGKTLLILSDKNIRPNYLPANALLATGAVHHH
                     LSSVGLRCDANIIVETATARDPHHFAVLLGFGATAIYPYLAHEIIADLARTGEVLGDP
                     IEAQAYFRKGINKGLLKILSKMGISTLASYRAAQLFEAVGLSDEVVKTCFKGVPSRIQ
                     GARFVDLENDQKQLAADAWKDRKPIDQGGLLKFVYGKEYHAFNPDVINALHDAVRSGD
                     YSKYQVYADLVNDRPVATIRDLLKLKTDNSITIDEVEPLESILSRFDSAGMSLGALSP
                     EAHESIAIAMNTLGGRSNSGEGGEDPARYGTLKNSKIKQVASGRFGVTPAYLTSAEVL
                     QIKVAQGAKPGEGGQLPGGKVNALIARLRHSVPGVTLISPPPHHDIYSIEDLSQLIFD
                     LKQVNPSALVSVKLVSEPGVGTIAAGVAKAYADLITISGYDGGTAASPLSSIHYAGSP
                     WELGLSEAHQALRINDLRGKVRVQTDGGLKTGLDVVKAAILGAESFGFGSTPMIALGC
                     KYLRICHLNNCATGVATQQDKLRANHYVGEPEMLMNFFKFVAQEARYWLAQLGVRSVA
                     ELIGRTDLLEMIEGQTVKQQHLDLMPLLKNDHITADKPQYCLQPKNAPFDKGVLAEQM
                     VAEILPAIQTKAGGEFSFKVGNCDRSIGARLSGEIAKLHGDQGMSTQPITVNLTGTAG
                     QSFGVWNAGGLNLVLEGDANDYVGKGMTGGKLVIYPPKGSPFKTQDTAIIGNTCLYGA
                     TGGKLFAAGTAGERFAVRNSGAHAVIEGAGDHCCEYMTGGLVTVLGRTGLNFGAGMTG
                     GFAYVLDLEGDFYDRCNHELIEIHRISTEAMEAHRHHLRSVLTEYVNETQSAWGQELL
                     AEFESYQRKFWLVKPKAANIKELLKNTRANPQ"
     assembly_gap    12353..12452
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12453..12604)
                     /locus_tag="PLY05_00645"
     CDS             complement(<12453..12604)
                     /locus_tag="PLY05_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01838.1"
                     /translation="MHMQPVAHSGLYQPDEFRDNCGFGLIAHMQGQASHHLVQTSIHS
                     LSCMTHR"
     gene            complement(12932..13639)
                     /locus_tag="PLY05_00650"
     CDS             complement(12932..13639)
                     /locus_tag="PLY05_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01839.1"
                     /translation="MLISRSRLWLPMAASSLAVLLISVTVISLKGRNAPQQTTIQDVF
                     TGEMHEFSADLGQLPKIVPPTNYVATPPVNHGPQYRTVDWLKAQGSLAWTLQIISSED
                     EEVVKSYIAQRDDKEQFAYFMYKEGEKVSYIAVYGNFVTMELALGVANSIDFALPNGV
                     RPSPEKFATYVANVPMIQPAIDQPPPPKYGGVPEIAIEPQPDDMAEPTQNQLETPADS
                     SSSSTEPEVSTPVVDPF"
     gene            complement(13641..14738)
                     /gene="aroB"
                     /locus_tag="PLY05_00655"
     CDS             complement(13641..14738)
                     /gene="aroB"
                     /locus_tag="PLY05_00655"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004877825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNC01840.1"
                     /translation="MQTLSVSLAERSYPIFIGESLLTKPECYQTYLKAKQVLIVSNTT
                     IAPLYLAKVEQTLQSLGYKVACHILPDGEQFKDLQHVEGIFKTLLEQRFNRDCALVAL
                     GGGVVGDMTGFAAACYQRGVAFLQIPTTLLSQVDSSVGGKTGVNHPLGKNMIGAFKQP
                     TAVLADTSVLDTLNDREYAAGLAEVIKYGLIRDLPFLEWLEQNMAGLVARDKKLLAEA
                     IARSCQNKADVVAADELEANDLRALLNLGHTFGHAIETAMGYGTWLHGEAVATGMLMA
                     ADLSWRLGWITQADVVRVKTILQAANLPVIPPTEMTSEQFLSLMAVDKKVLAGKLRLV
                     LLRQLGQAVVSADFPLQTLEQTLTAGTELGQ"
     gene            complement(14820..15338)
                     /gene="aroK"
                     /locus_tag="PLY05_00660"
     CDS             complement(14820..15338)
                     /gene="aroK"
                     /locus_tag="PLY05_00660"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HNC01841.1"
                     /translation="MAACANIFLVGPMGAGKTTIGRHLAEILSWPFVDSDHEIELRTG
                     ASIPWIFDVEGEEGFRKREEQMIAELTNRRHIVLATGGGAVLRQINRQNLHQRGVVVY
                     LETPVSMQLERTAHDKNRPLLQTADPQTKLAELLKIRDPLYREVAHLIVPTTSGCAKD
                     LAYSIIQKLNFI"
     gene            complement(15351..17486)
                     /gene="pilQ"
                     /locus_tag="PLY05_00665"
     CDS             complement(15351..17486)
                     /gene="pilQ"
                     /locus_tag="PLY05_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010589334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HNC01842.1"
                     /translation="MSIQRMVLGVGLMSVLQLAAAAERLTLKNVNFITLPGDEIEVKL
                     AFDDAPPQLQSYQIEKPARLVFDLPNTKNGLNSRYLSLGNGNARSLTVVDNQERTRVV
                     VNLGELTGYATRVEGNDLILKIGKQDITNNVAQAVPIVEKIKVSPKQKASMPKVLQVD
                     DLDFHRGLQGDGQIDIQLSQASIPVDVQQQGSKIIARFIGAKLPEKLRRRLDVNDFAT
                     PVKTIDAYNEGNNGVIVIQPQGEFEYLAYQADNNMTISVKPILPDAPKKRKEFVYTGE
                     KLSLNFQDIEVRSVLQLIADFTSLNLVASDTVSGRITLRLQNVPWDQALELILKTKGL
                     DKRTTGNVMLVAPADEIAARERLELEAANQNEQLAALRSEYIQVSYAKAADLQKMITS
                     GINTSNSANTASNSLLSPRGSVSIDVRTNTLIIQDIPKKIEEIRDLVSRLDVPVKQVM
                     IEARIVTASSDFVKELGVKWGVAHDNISSKKRVMVGGSRTTLNDIREATIDTVAGTRD
                     YAIASPSDLAVDLGVEKVGTSSLAVGIMNADNIVLDLELSALQSDGHGEVVASPKVLT
                     ADKQKALIASGTQIPYGESTSSGAAAVKFINAELRLEVTPSITPDGRVNMDLAINKDS
                     PGAVLSNGALTINSNRIATSVLVDDGQTVVLGGVFTTDMLKGVTKTPLLGDIPFLGRL
                     FKQDVTRNEKKELLIFVTPRLLNDTIASK"
     gene            complement(17558..18097)
                     /locus_tag="PLY05_00670"
     CDS             complement(17558..18097)
                     /locus_tag="PLY05_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004991753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HNC01843.1"
                     /translation="MKRILRKVIALSMAVITSFGVVGCSSDFDTMKARMDAIRAKPRG
                     RIEPPPEFTPMPTFAYAAHQLRSPFVPPSTLEELLAKASGKQVAPDFSRPQEYLEKFG
                     LEALRMKGTLTKPSAPFYALVEDADGGVQRVKVGNYMGKNHGKIVEITPSQINLVEVV
                     PDGRDGWVERPRSVVMADK"
     gene            complement(18097..18810)
                     /gene="pilO"
                     /locus_tag="PLY05_00675"
     CDS             complement(18097..18810)
                     /gene="pilO"
                     /locus_tag="PLY05_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HNC01844.1"
                     /translation="MKLKTDKAEAQSDVKKFDLQEFINTLNTLDPQNVGSWPLAAKIL
                     VYIIVFILVLVLGYVFDLSPMRETYASGEQAQETLLQEFEQKVFKANNLDVYKKQLAD
                     MEESFGSLLRQLPKETEVPELLEDITHTGLGSGLDFDGITLETESTQEFYVEQPIKIK
                     VQGDYHAFGAFVSGISALPRIVTLHNFTVVPMANKFADSGAPILTMEILAKTYRYKDT
                     AEGDANKKSTPTDQKKEGK"
     gene            complement(18807..19433)
                     /locus_tag="PLY05_00680"
     CDS             complement(18807..19433)
                     /locus_tag="PLY05_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HNC01845.1"
                     /translation="MAKINLLPWRQELKKQRQQEFVAINAGVALAAAAIVLFFHMMLS
                     AQLSDQEERKAYIQSEIAMLDSQIKQIDELQTRKEELLARMKVIQDLQGRRPIIVRVF
                     DELVRSVPNKVYLLSVKRDGDKFTIEGNAESNTQVSSFLRNLNASEWFKNPVLSTVTA
                     DDTVKKATKVSLDKRSNRFALTVELEGPEPIKTDDKTGVAKTNKEAAK"
     gene            complement(19433..>19665)
                     /gene="pilM"
                     /locus_tag="PLY05_00685"
     CDS             complement(19433..>19665)
                     /gene="pilM"
                     /locus_tag="PLY05_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004725424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNC01846.1"
                     /translation="FFSSSQYNDVDHILLAGGTASIGGLADVVQEKLGTAVSVANPFV
                     HMSFAPQINAAAISNDAPSLMIACGLALRSFD"
BASE COUNT         5835 a         4295 c         3507 g         5728 t
ORIGIN      
        1 ttaaataacc ttgtcctgtt aactgccacg gtgcaggcgc aattttgatg ctcatattta
       61 cctctagatt aacccctcct cctaatgtgg aaagagcttg agtgggatta cagcataaaa
      121 cgatttttgg ttttgtttat tgtgggttac ggtcaacgtg tatttgcccc aacaatagtg
      181 gcatcggaca agccaacaaa gaggctaaca ctcttaataa ttcatattcg gcacgatgca
      241 aaataccatc actttgtata gttgccgcag ctaaagtaat taattgctgt tttgcctttg
      301 gagttaaata ttgcaactgc tgtaaggctt tggccaatac tgtaaaatta tctttttcaa
      361 taacaggcat gactggcgtg tgttcggcaa acatggtggg cattaattgc tgataaattt
      421 gcttttgtaa atgaatagga tgcgcgccgt aatgtaataa tgttgctatt aattcggcac
      481 aaggttgcgc taaacgagtt aaaggcaatt ggcgaagaat cgtcttagtt ttgcttaact
      541 gctgctcaat taaacttaat aaggcaaact caaacacatt atctatttgg ccaacaaaac
      601 gttttaattc actaataaaa ctttgctgct cttgctcggt taaacgttgt aaacgtggaa
      661 tcgctaattc aacgactggt aaagctaaac gtgtatccac cgttgttaag tgctgttgta
      721 gttgaataat cgtttgtgca aatgcaggct gttggttaaa aaaatcgcga ataaactgcg
      781 ttggtgtgtg ttgttgattg gcaactaagg caaaaatcac cgcttttgcg ccttgagtgc
      841 cttgtaaagc acgactaact tctaccgcca attgtcgatg taaatactgg gctgacatta
      901 agtcttgcca ttgtaaatcc cccactttgc ttgcggtcat cgttgcggca ataataggta
      961 actcaactgc actaaaggca atcaaaccat ctgcttgtat ggatacaggt atactggccg
     1021 atgtttttgg ctgtggctct tctactttta tggggtcttg ccgatatttc atccgtgtta
     1081 aaaaacttgg cgaaatgata ttaattcgct gtttaatggg tggatgactg gctaaatatg
     1141 gggcaaataa cacactatca gcaaaacaca tatgactgag ttgttcggca taacgatttt
     1201 gcaataaaga gccatgttga ctaattttgt ataaagcact ggctaaggca tctggataac
     1261 gagtaaattg cacactggct gcatcggcaa gattttctcg actacgcgaa atggctgctt
     1321 tgactaatcg ggcaaaaaac agtcctatat aaccaattag ccaaataatc aaaccaacca
     1381 cataaataat gggcatcggc caaatttttt taggcttaat ctgcttttgc gagcctagat
     1441 aactcaacct aattaaataa tcgcctgctt gaccaatatt gacaatgcct gccaaaatcg
     1501 acactaaata cgtgtttaag cgcatatcgc cattaataat atggctgtac tcatggccaa
     1561 tgattgcttg caactcgtcc cgattcaaat gctccaacgt accttgtgtc accactaaaa
     1621 tactatcttg aaggttatac cctgctacaa aagcattaat cgttttttct tgatgtaaca
     1681 cataaagact gggcatagtc atgcctgctg caatcgccat ttcttcacaa acattgacta
     1741 attgctgttg ttgagagtct ttactggcaa aatctatttt attcgcctga atcatattgg
     1801 ccaaagcgac accaccctca cgcaacaaaa accattcaac catgctaccg ctagcaatta
     1861 aactaactgt tgcaccatag gctttagctg accaaacatg ccacaaccat gaaccttgtc
     1921 catcgtcata acctgttaat attgcgacaa aatagaagaa tatattgaca gcaaccgcaa
     1981 ttaacaccat taccaacact aaatatatta ataaatagcg tgtggcggag cgtgcttgtt
     2041 gttgccgtcc aaaaaaatca aacatgccaa tctatccatt aaaagaaatt ttgactggct
     2101 ctttggcttc agaatggcta atttttaaca atgttgcttc ttgaaagccc gtcatattgg
     2161 caacaagatt attaggaaac ttttcacgtt cggtgttgta aatcatcact gcatcattaa
     2221 atgcttggcg agcaaaactg actctatttt cagtgctttt taattcttcc attaagtttt
     2281 gaatggtgct attggcttta atatcaggat acgcttcgga tatagccatc aatcgcccta
     2341 aagcttgatt taacacccct tctgcttcgc ctaattgatt aatcgcatat tgattatcac
     2401 tatttttagc tgccatttgc tcaacagtaa tcgcttgatt acgggcatta atcacttggg
     2461 ttaaagtgtc tttttcgtgg ctgagataag ctttcacggc ttcaaccaaa ttagggatta
     2521 attcatagcg acgttgtaat tgcacatcta tttgtgcaaa agcattttta aattgattac
     2581 gtagactaat taaaccgtta taggcattaa tcccataaac aatcgccaaa attaacattg
     2641 cccaaaaaaa caatgacatc atctttcctc cgtgtttttt ttagattaaa tcactgaatg
     2701 acaccttaaa atgttttttc ttgttatgca agaacaggta caatacgcgg tttgtnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacacc ttaaaatgtt ttttcttgtt
     2881 atgcaagaac aggtacaata cgcggtttgt tttgtgtgtc tgtttttgga gtgcgctcat
     2941 gtctgttcaa caacccaaag taggttttgt gtctttaggt tgccctaaaa atttggtcga
     3001 ttctgagcgc attttgacgc aattacgtgc cgaaggttat caaatttcgc ccgattataa
     3061 cggggctgat ttagtggtgg tcaatacctg tggctttatt gagtctgcgg tacaagagtc
     3121 cttagatgcg attggcgaag ccttagccga aaatggcaaa gtcattgtca caggttgttt
     3181 aggcatcaaa gaaggccaaa ttcgtgacgt gcatcccagc gtgttaaaag tgactggccc
     3241 acacgcctat gaagaagtga tggacagtgt gcatcaatat ttaccaccga aaaaagatca
     3301 taatccgttt attgatttag tgcccgctca aggcatcaaa ctcaccccta agcattatgc
     3361 ttatgtcaaa atttctgaag gctgcaacca tcgttgtacc ttctgtatta ttccgtccat
     3421 gcgtggtgat ttagtgtctc gcccgattgg ttcagtgatg gccgaagccc aaaacttggt
     3481 taaatcaggc gtaaaagagt tattagtcat ttcgcaagat acctctgctt atggcgtaga
     3541 tttaaaatac aaactcgatt tttggaatgg ccaaccgtta aaaacacgta tgcaagaatt
     3601 gtgtgaagca atgtctacgt taggtgcttg ggtacgtttg cattatgttt atccatatcc
     3661 gcacgttgat aacgtcattc cgttaatggc tgaaggcaaa attttgccct atttggatat
     3721 tcccttccaa cacgccagtc cacgtattct gaaattaatg aaacgtcctg ctcacagcga
     3781 aaacaccctc gaacgcatta aacaatggcg caaaatttgc ccagatttag tgttacgttc
     3841 tacctttatt gttggctttc ctagtgaaac cgaagaagag tttgaagagt tattaaactg
     3901 gttacgcgaa gcacaactcg accgtgtggg ctgctttaag tattcacctg ttgacggtgc
     3961 agcggctaat gaattgccag cccccattcc agaagacgtt aaagaagcgc gttggcaacg
     4021 ttttatggaa gttcaacaag aaatttctgc tcaacgttta caattaaaaa ttggcaaaac
     4081 cattcaagtg ttaattgatg acattgatga agaggaaggg gttgctattg ctcgttcagc
     4141 agcggatgcg cctgaaattg atggcaatgt gtatattgaa ggcgaaggcg cagccgattt
     4201 aaaaattggt gattttgcca ctgtcacgat cattgatgcn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnt tagataggat attgccatgc gtaaattacg atttttaggt
     4381 gcagcccaag aagtaaccgg ctcttgttat ttgctcgaaa atgcaggcca tcacatttta
     4441 ttagattgtg gtattcatca aggcggtgat ggtgttgacc gaattaccaa agaagaattt
     4501 aagtttgctc ctgccagtat tgatgccgtt gtcttatctc atgcccattt agaccactca
     4561 ggcttattac ccaaactcgt tcatgaaggt tttaaaggca aaatttattg tacgcatggc
     4621 acacataaac tgttaaaaat tttattagaa gatgcggttc atatttattt aaaagatttg
     4681 gaatatgaga acttacgccg tgaacgtgca gggaaaaagc ctttatttcc gcaatatacc
     4741 ttagatgatg tgcaaacggt tttaaattta tgttgtgcct tagactatca tcaagcacat
     4801 acttttttag aaaaatttac cctcaccttt tatgatgcag ggcatatttt aggttcatcg
     4861 attgtgcagc ttgtactcaa tgataatcac aacgcaaaaa ctttagtatt ttcgggtgat
     4921 ttgggtaatc ccgatacctc cttaatgcgt aattttgatt ttattgataa tgctgatgtg
     4981 gtgttaatgg agtcaactta tggtgatcgt gaccatcgtt gttttgccga aactttagac
     5041 gaatttaaag ccattttaaa acaagccgaa caagatggcg gcaatatttt aatccctgct
     5101 tttgctgtgg gtcgtaccca agaattattg tttcacttag gcaaaatgta tcacgaaggt
     5161 ttattagatc attggcatat ctttttagat agcccaatgg ctaaagcggt gaccaatgtt
     5221 tatagcgact gtattaaaca attagacaaa gaagacgttg cctttattac agcgcatcaa
     5281 ggtgaagata ttagctcatt tttgcccaat ttaaccatta gtgaaagtgt cgaagactcg
     5341 gctgcgatta ataatattaa aaaaggcgcg attattattg caggtagcgg catgtgtaca
     5401 ggtggacgca ttcgccatca ttttaaacac cgtttatggc acaccaatac ccatgtggtg
     5461 tttgttggct ttcaggcgcg tggtactttg ggcagaattt tggtcgatgg agttaaacac
     5521 gttaaactgt ttggtttacc gattttagtc aaagcacaaa ttcatacttt gggtggattt
     5581 tcggctcatg cagggcaaaa agaattgtta gcatgggcaa aaaactttaa aacgcaacca
     5641 cgtttttact tggtgcatgg tgaagcaaaa accatggaaa agttaagtga aaaacttgca
     5701 caggaacttg gcattaatgc cgaaattcct gcgcaactgt cacagattat gttttagatt
     5761 tttatagagg ttggtctttt aaaatcaacc tcttaattta cagctattaa atcacaaaac
     5821 caaaagcctt gggtttgatt atcactgcta caaacggcat cggtactttt aatttctaac
     5881 ggtttatata ccttttcgcc aagttcaaag gcaacagcat gcttaaaata tggagcatca
     5941 taacaaaaag tatgatactc accattttca ccacaagcat ccacttgagc aggtaactca
     6001 gcaacaaatt gctgattaat ttctcgcccc acccattgtt catttaaata agcatcgctc
     6061 acacaacagg taattgtttt aaaaccttga gcaataaaat cattaatcaa ttgcgtagta
     6121 tttttttgcc ataacggaaa aaccgcttct aagcccactt gcgccaagtt tttttcgcga
     6181 taggcacgta aatcggctaa gaaaatatcg ccaaaaatga catggctaat accttcggct
     6241 tttgcctgta atagcgtttg tgccatttgt tgttcatatt cactatttga accttgataa
     6301 acctttactt ttaataaagg aatcccaata gctgaggctt gctgttctaa taataattcg
     6361 cgcacaccat gcatagaaat acgctgaaac tcaccattaa tcgtggttaa taagtactta
     6421 acctcatact cacctgcttg taaaatttgg tgtaaggcat aagcagaatc tttaccgcca
     6481 ctccaacaaa aaatagcttg tttcataatt tggatttcac attaatatgt tgataaggac
     6541 aatgtcgaca accatttcca caacaagagc cacgtttacg gtgataccac tcggtaaata
     6601 cccatttacc atcttcaaga taataatcaa tatcttcaat gagttgtgca ttatgatatt
     6661 gcttaggaat tgcgactgtc actggatttt gttgtacaaa ttgctcaatg ccttgtttaa
     6721 tcaccacctt caaacattgt gggcagcgac aatcttgttc aaagtcgagc ggcataatag
     6781 cgggcaactg attacaccaa caggcttgct ggccagtgtt gccacaggca aaagccacac
     6841 cacaaaccga acattttttt tgcatgatta gattatcctt tggtaataaa aaaggcgcat
     6901 tttctgcgcc ttttttattg gtctatggct ttaaacttaa acgtttaaat aatccaaaat
     6961 accttcggca gcttgacgac cttcccaaat cgctgtcacc acgaggtcag aaccacgaac
     7021 catatcacca cccgcaaaca cttttggatt gcttgtttga tatttaaatt cagactcggc
     7081 aggtgcaact acacgaccgc tattgtctaa attaatacca aaatcagcaa accaaggtgc
     7141 tggacttgga cggaaaccaa acgccaaaat caccgcatca gcaggtaata cttgttcaga
     7201 atttggaatg ggctctgggc tacgacgacc acgcgcatct ggtgaaccta aacgggtttc
     7261 aacgactttt acgcctgtta caccgttttc atcacccaag attgcaattg gttgacggtt
     7321 aaacaagaag ttaacaccct cttcacgcgc attatttact tcacgacgtg aacctggcat
     7381 gttttcttca tcacgacgat aagcacaggt cactgcatct gcaccttgac gaatcgaggt
     7441 acggttacag tccattgcgg tatcaccacc acctaaaaca acaacttttt tacctttcat
     7501 ggagataaaa tcgttagcgt ctttttccca accattgtta tggttgacgt tggcaatcaa
     7561 gaaatctaaa gcgtcataga cgtttggtaa atcttcaccc gcaaagccac ctttcatata
     7621 ggtgtaagtg cccatgccta aaaatacagc atcaaattca ttgagcaaat cagtgaattg
     7681 aacgtcttta cccacttcgg tatttaaacg gaactcaata cccatatcgg taaagatggt
     7741 gcgacgacga gacatcacac ttttttccat tttaaattca ggaataccaa aggttaacaa
     7801 accaccaatt tcagggtttt tatcaaaaac aacaggttta acaccactac gtactaacac
     7861 atcagcacaa cccaaacctg ctgggcctgc accaataatg gcaacacgtt tgtttgtcca
     7921 tttaactttg gacatatctg gacgccaacc catggcaaag gcggtatcaa cgatatattt
     7981 ttcggcatta ccaatagtca ctgcaccaaa accgtcattt aaggtacaag caccttcaca
     8041 taaacggtct tgtgggcaca cacggccaca cacttcaggc aaggtattcg tttggtgaca
     8101 taattctgct gcaccaaaaa tattaccttc cgaaattaac tttaaccagt tgggaatgta
     8161 gttatgaaca ggacatttcc attcacagta aggattacca caagctaagc aacgatgtgc
     8221 ttgttcgcct acttctactt tagtaaaaga gtgataaatc tctacatatt cgcttttgcg
     8281 aacggcgata tctttttttg cggggtcatg ccgcgcgacg tctaagaatt gaaagtcgtt
     8341 atttaggcgt tctgccatgg ccttgcctca atatatatct agctaggtta aaccatcccc
     8401 ttaactcccc taagttttta gagggagttc cctctccttt taggagaggg ctagggtgag
     8461 gttaaaatta ctgtggatta gcacgagtat ttttcagcaa ttctttgatg ttagcggcct
     8521 ttggcttcac taaccagaat ttacgctgat aggactcaaa ctcggccaac aattcttgac
     8581 cccacgcact ttgcgtttcg ttaacgtatt cggttaacac actgcgcaaa tgatgacgat
     8641 gagcttccat cgcttcagtg ctaatacgat gaatttcgat taactcatgg ttgcaacgat
     8701 catagaagtc accttctaaa tccaacacat aagcaaaacc gcctgtcata cctgcaccaa
     8761 agtttagacc tgtacggcct aatacagtga ctaaaccacc tgtcatgtat tcacaacagt
     8821 ggtcgcctgc accttcgata acggcatgtg caccagagtt acgtacggca aaacgctcac
     8881 ctgctgtgcc cgctgcaaat aacttaccgc cagttgcacc atacaaacag gtgttaccaa
     8941 taattgcggt atcttgtgtt ttgaatggcg aacctttagg tggataaatc actaatttac
     9001 cacccgtcat gcctttaccc acataatcgt tggcatcacc ttccaagact aagttcaagc
     9061 cgcctgcgtt ccaaacacca aaactttgac ctgctgtacc tgttaagttt acggtaattg
     9121 gttgagtgct catgccttgg tcgccatgta atttggcgat ttcaccactt aaacgcgcac
     9181 caatcgagcg gtcacagttg cctactttga aactaaactc accacctgct ttggtttgaa
     9241 tggcaggaag aatttcagca accatttgtt cagccaatac gcctttatca aagggtgcat
     9301 ttttgggctg caagcaatat tgtggtttgt cagccgtgat atggtcattt ttcaacaaag
     9361 gcattaaatc taaatgctgt tgttttacgg tttgaccttc aatcatttct aacaaatcgg
     9421 tacggccaat caactcagca accgaacgta cgcctaattg tgctaaccaa taacgcgctt
     9481 cttgggcaac gaacttaaag aagttcatta acatttctgg ctcgcccaca taatggttgg
     9541 cgcgcaactt gtcttgttga gtcgcaacac ccgttgcaca gttgtttaag tgacaaatac
     9601 gcaagtattt acaacccaaa gcaatcatag gtgtcgaacc aaaaccgaag gattcagcac
     9661 ctaagatagc tgctttaaca acatctaaac ctgtttttaa accaccatcg gtttgcacac
     9721 gcactttgcc acgtaaatca ttgattctta aggcttggtg agcttcggac aaacctaact
     9781 cccacggaga acctgcgtag tgaattgacg ataatggcga tgcggcggta ccaccatcat
     9841 aacccgaaat agtaattaag tcggcatacg cttttgccac acctgcggca attgttccca
     9901 caccgggttc agacaccaac ttaaccgaca ctaaagcact ggggttaact tgttttaagt
     9961 caaaaattaa ctgtgataag tcttcaatcg aataaatatc gtggtgtggt ggtggagaaa
    10021 tcaaggtaac acctggtaca gagtgacgta aacgcgcaat caaagcattt actttaccac
    10081 caggtaactg accaccttca cctggtttag caccttgtgc cactttaatc tgcaacactt
    10141 cggcagacgt taaataggca ggtgtcacac caaaacgacc agaagccact tgtttgattt
    10201 tcgagttttt cagcgtacca taacgtgcag ggtcttcacc accctcaccc gaattggaac
    10261 gaccacccaa ggtattcatt gcaatcgcaa tcgattcgtg cgcttcagga gataatgcac
    10321 ccaaagacat ccctgcggag tcaaagcgtg acaaaataga ctctaatggc tcaacctcat
    10381 caatagtaat gctattatct gtttttaatt tcaacaaatc acgaatggta gctacaggac
    10441 ggtcattaac caaatcagca tatacttggt atttgctgta atcacccgaa cgaacagcat
    10501 cgtgtaaggc attaatcacg tctgggttaa aggcatgata ttctttgcca tagacaaatt
    10561 tgagcaaacc accttggtcg attggcttac ggtctttcca cgcatcagct gccaattgtt
    10621 tttggtcatt ttctaagtca acaaaacgcg ccccttgaat acggcttggt acacctttga
    10681 agcaagtctt caccacttca tcacttaaac ctaccgcctc aaataattgt gcagcacggt
    10741 aagacgctaa ggtagaaata cccatcttcg ataagatttt taacaagcct ttgttaatgc
    10801 ctttacggaa ataggcttgt gcctcaatgg ggtcacctaa gacttcacct gtacgtgcta
    10861 agtcagcaat aatttcatgt gccaagtatg ggtaaatggc tgttgcacca aagcctaaca
    10921 acacagcaaa atgatgtgga tcacgtgcgg ttgcagtttc aacaataata ttggcatcac
    10981 aacgtaaacc aacactgctt aagtggtgat gaactgcacc tgttgccaat aaggcattgg
    11041 ctggtaaata gtttggacga atgtttttat cagacaaaat caacaaggtt ttgccatttt
    11101 taacagccgt ttccgcttca tcacaaatac gtgcaatggc tgcttgcaaa ccttcacttt
    11161 cagcatagtt caaatcaata atagcgtttg cataaccttt ttgatcattc aacgctaaaa
    11221 tttgttggaa cttgctaaac gacaacacag gactggccaa aattgcacga tgtgcatgct
    11281 ctgctgtttc ttcaaaaaca ttcatttccg cgccaaggca agtctctaaa gacatgacaa
    11341 tagcttcgcg caatgggtca attggcgggt ttgtcacttg ggcaaactgt tgacggaaat
    11401 agtcagccat gtgacgtaca cgacgagaca ataccgccat tggggtatca tcacccattg
    11461 agccaacagc ttcttgacca ctttctgcca atggacgaat aatctgatca cgctcttcaa
    11521 aggttaccaa gaacattttt tggtaagttt tgagctgagc agcatccatt tgtcccaagc
    11581 cgcgcttatc aaccacatcg tcattctcta aacgaatcgc ttgatcacgc aaccatgttc
    11641 tataaggatg agctgatttt aagcgttggt cgatatctgc tgtacgcaac aattcgcctg
    11701 tttgagtatc aactactaaa atttggcctg gcccaacacg accttttgat accacatcgt
    11761 caggctggta atcccaaacg cctatttcag aggctaaggt gatataacca tttttagtaa
    11821 ttacccaacg tgcaggacgc aaaccgtttc tgtcgagcat acaaatcgca tgacgaccat
    11881 cttgaattac caaacctgct gggccatccc atgcttccat atgtttagag ttgtattcat
    11941 agaaggcgcg caaatcagca tccatcgttt ccacgttttg ccatgcgggt ggaaccaaca
    12001 tacgcaaagc acggaacaag ttcatacctc cacccactaa aatctctaac atattatcta
    12061 agctagagga gtcagaacct gtacgattca ccagtggttt acaatctaat aaaccaggca
    12121 acaatgggct ttcaaatttg ggcgtacgtg caaccgacca attacggtta cccataatcg
    12181 tattgatttc accattatgc gccaaatagc ggaacggctg cgccaatggc cactttggca
    12241 aagtattggt agagaaacgt tgatggaaca ccacaatatg tgaggccaaa cgttcatctt
    12301 gtaaatcttt ataaaaactt ggcaaatccg caggcatcac caagccttta tannnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncggtgcgt catacaactt aaactatgaa
    12481 ttgaagtttg tacgaggtga tgactggctt gcccttgcat atgggcaatc aaaccaaaac
    12541 cacagttatc ccgaaactca tcgggttgat agaggccaga atgggcaacc ggctgcatgt
    12601 gcatggcttt accttaaaaa atatggactt ataaaaaaca cccagcatcg atatttcgat
    12661 aacctgtggg cattttttag aacttacacg cacagcataa gttgtctatt aaaaatgctt
    12721 taggaagatc aaagtacctg cttaagccct agaaactcaa agtttgccca caacaaacgg
    12781 tattttattg cggacaaaat ggccaagtat tttatatata aattgcctat gactcaaaca
    12841 ctacaagcaa aatctgccat ttttttgcat aaaaattact ttttttgcat ttttcatcgt
    12901 ttaactcacg attataggca aaatactttg actaaaaagg atcaaccaca ggcgtcgata
    12961 cttcgggttc tgttgatgat gaagacgaat ccgcaggagt ctcaagctga ttttgagttg
    13021 gctcggccat atcatcaggc tgaggctcta ttgcaatttc tggtacacca ccatatttgg
    13081 gtggaggtgg ctggtcaatt gctggctgaa tcattggcac attagccaca taagtcgcaa
    13141 atttttcagg agatggtctc actccattag gcaatgcaaa atctatagaa tttgctacac
    13201 ctaaggctaa ctccattgtg acaaaattac catacaccgc aatataactg actttttcac
    13261 cctctttata cataaagtaa gcaaattgct ctttatcatc gcgttgcgca atataagact
    13321 ttaccacttc ctcatcttct gaagaaataa tctgcaaagt ccacgccaac gaaccctgag
    13381 cctttaacca atcaacagta cgatattgtg ggccatgatt tactggtggt gttgcaacat
    13441 aattcgttgg tggcacaatt tttggtaatt gccctaaatc agctgaaaac tcatgcatct
    13501 cacccgtaaa tacatcttga atcgtagtct gttgaggagc attacgtcct tttaaactaa
    13561 taaccgtcac actaatcaat agcactgcta agctgcttgc agccattggc aaccataatc
    13621 gtgatcgact tattaacata ttattgcccc aattcagtgc ccgcagttaa ggtttgctct
    13681 aaagtttgca aaggaaagtc tgcactcact actgcttgtc ctaattgacg caacaagact
    13741 aaacgcagct tacctgccaa cactttttta tcaaccgcca tcaacgataa aaactgctca
    13801 gatgtcatct cggtaggagg aataactggc aaattagccg cttgcaaaat tgttttaact
    13861 ctaacaacat ctgcctgtgt tatccagccc aatcgccacg ataaatcagc agccattaac
    13921 atacctgttg ctactgcttc cccatgtaac catgtaccat aacccatcgc cgtttcaatc
    13981 gcatgaccaa acgtatgccc caaatttaat aaagcacgaa ggtcattcgc ttctaactca
    14041 tcagccgcga caacatcagc tttattttga caagagcgcg caatagcttc agctaataat
    14101 tttttatcac gagcaactaa gccagccata ttttgctcaa gccactctaa aaaaggcaaa
    14161 tctcgaatta agccatattt aataacttct gctaaacctg ctgcatattc tcgatcattt
    14221 aatgtgtcta aaacagaagt atcggccaat acagccgttg gttgcttaaa agcaccaatc
    14281 atattttttc ctaatggatg attaacaccc gttttccccc ctactgaaga atcaacttgt
    14341 gataataaag tggttggaat ttgtaaaaac gcaacaccac gctgatagca tgcggcagca
    14401 aagcccgtca tatcaccaac cacaccacca cccaatgcta ctaaagcaca atcacgatta
    14461 aaacgttgct ctaacaatgt tttaaagata ccttctacat gttgtaaatc tttaaattgc
    14521 tctccatcag gcaaaatatg acaagcaact ttatagccca agctttgtaa agtttgttcg
    14581 acttttgcca aatataaagg tgcaatcgtt gtattactca caataagcac ttgtttagct
    14641 tttaaataag tttgatagca ttcaggcttg gttaataaag actcaccaat aaaaatagga
    14701 taactgcgct cggctaaaga gacggataat gtttgcataa caaagaattc ttaacaggcc
    14761 aaattaaata tggattttaa cggaattatt agttttataa acaaaaattg gaaagcagtt
    14821 taaatgaaat taagcttttg aatgatgcta taagctaaat ctttagcaca accactggtc
    14881 gttggcacaa taagatgtgc aacttctcga taaagtggat ctctaatttt aagtaactct
    14941 gctaatttag tctgaggatc agccgtttgc aataaaggtc gatttttatc gtgagcagta
    15001 cgttcaagct gcatagagac aggggtttct aaataaacta caaccccacg ttgatgtaaa
    15061 ttttgacgat ttatttgcct taatactgca ccaccacctg ttgctaacac aatatggcga
    15121 cggtttgtca gttcagcaat catttgctct tctcgctttc taaacccttc ttctccttca
    15181 acatcaaaaa tccaaggaat actagcacct gtgcgaagct ctatttcatg atcactgtca
    15241 acaaatggcc aagataaaat ctcagccaaa tgtcgaccaa tagtggtttt acctgccccc
    15301 attggtccga ccaaaaaaat attcgcacag gcagccaatc ttattaccta ttacttgcta
    15361 gcaattgtgt catttaacaa acgcggcgta acaaaaatca ataactcttt tttctcgtta
    15421 cgtgttacat cttgtttaaa taaacgccct aagaatggaa tatcaccaag caatggcgtt
    15481 tttgtgacac ccttgagcat atccgtggta aaaacaccgc ctaacactac tgtttgtcca
    15541 tcatcaacta agacacttgt tgcaatacga ttactgttaa tagttaatgc accatttgat
    15601 aatactgcac ctggactatc tttattgatt gctaaatcca tatttactcg cccatcagga
    15661 gtaatactag gcgttacttc taaacgcaat tcagcattaa taaactttac tgctgccgca
    15721 ccacttgatg ttgattctcc ataaggtatt tgagtgcccg aagcaattag tgccttttgc
    15781 ttatctgcgg ttaagacttt tggactagca acaacctcac catgtccatc agattgtaaa
    15841 gcagataatt ctaaatctaa cactatatta tctgcattca taataccaac agccaaagaa
    15901 cttgtaccaa ctttctcaac acctaaatcg actgctaaat cagatggact agctatcgca
    15961 taatcacgag tacccgcaac tgtatctatt gtagcttcac gtatatcatt taaagtagtg
    16021 cgcgaaccac ccaccataac tcgtttcttt gagcttatat tatcgtgtgc aaccccccac
    16081 tttacaccta attctttaac aaaatcacta gatgcggtga caatacgagc ttcaatcata
    16141 acctgcttca caggaacatc taatctagag acaagatccc taatttcttc tattttctta
    16201 ggaatatcct gaatgatcaa tgtattagta cggacatcta tagaaacact accacgtgga
    16261 ctcaacaaac tattagacgc agtattcgct gaattagatg tattaatacc cgatgtaatc
    16321 attttttgta gatcagctgc ttttgcataa ctgacttgta tatattccga acgcaatgca
    16381 gcaagttgtt cattttgatt tgctgcctcc aattctaaac gctcacgagc agcaatttca
    16441 tccgccggag caaccagcat aacgttaccc gtagtacgtt tatctaaacc tttagtcttc
    16501 aaaatcaatt ctaaagcttg atcccatggc acgttctgta aacgcaaagt aattcggcca
    16561 gacactgtat cactagcaac taaattcaat gaggtaaaat cggcaatcag ctgtaaaact
    16621 gagcggactt caatatcttg gaaatttaaa gataactttt cacctgtgta aacaaactct
    16681 ttacgctttt taggtgcatc aggcaaaatt ggcttaacac taatggtcat gttgttatca
    16741 gcttgataag ctaaatattc gaactctcct tgtggctgaa taacaataac accattattc
    16801 ccttcattat aagcatcaat ggttttaaca ggtgtggcaa aatcattaac atctaaacga
    16861 cgacgtaact tttctggtaa ttttgctcca ataaaacggg caataatttt actaccttgt
    16921 tgttgaacat caacaggaat tgaggcctgt gataattgaa tatctatttg cccatcacct
    16981 tgtagaccac gatgaaaatc taaatcatca acctgtaaaa cttttggcat tgacgctttt
    17041 tgcttggggg atacctttat tttttcaaca ataggcactg cctgtgcaac attattagta
    17101 atatcttgct taccaatttt aagaataaga tcatttcctt caacacgtgt agcatatcct
    17161 gttaattcac ctagatttac cactacacga gtccgctctt gattatcaac aaccgttaaa
    17221 cttcgagcat taccattgcc caaacttaag taacgagagt tcaaaccatt tttggtatta
    17281 ggtagatcaa aaaccaatcg tgcaggcttt tctatttgat atgattgtaa ttgcggtggc
    17341 gcatcatcaa acgctaattt aacttctatt tcatcaccag gcaaagtaat aaaattaaca
    17401 ttcttcaatg tcaagcgttc tgcagcggct gcaagttgca acacagacat caaacctaca
    17461 cctaatacca tacgctgtat agacatgctc tttttcccag ctggcttgtt attgttcatt
    17521 gtgatccgtt tgatcattta aattctccaa gtcttgacta tttatcagcc atgaccacac
    17581 tgcgtggacg ctcgacccaa ccatctcggc catcaggaac cacttcaacc agattaattt
    17641 gactcggcgt aatttctaca attttgccgt gatttttacc catatagttg cctactttga
    17701 cacgctgaac accaccatca gcatcttcaa ctaatgcata gaatggtgca cttggtttag
    17761 ttaaagttcc cttcatacgt aaggcctcta aaccaaactt ttctaaatac tcttgaggac
    17821 gtgaaaaatc aggggcaact tgctttccag aggcttttgc taacaactct tcaagtgtcg
    17881 aaggtggcac aaaaggacta cgcaactgat gtgcagcata ggcaaaagtc ggcatgggcg
    17941 taaactcagg cggaggctct attcgccctc taggcttagc acgtatggca tccattcgag
    18001 ctttcatcgt gtcaaaatcc gaagaacatc ccaccactcc aaacgaagta ataaccgcca
    18061 ttgaaagagc aataacttta cgcaatattc gcttcattat ttaccctcct tcttctgatc
    18121 cgttggtgtg gattttttat tagcgtcacc ctcagcagta tctttataac gataagtttt
    18181 cgctaaaatt tccatcgtca atatcggagc accgctatct gcaaatttat ttgccattgg
    18241 cactactgta aaattatgca aagttactat tcttggtaat gcagaaatac cactaacaaa
    18301 tgcaccgaaa gcatgataat cgccttgaac tttgatttta ataggctgtt ctacataaaa
    18361 ctcttgggta ctttccgttt ctaaagtaat accatcaaaa tccaagccac tacctaaacc
    18421 agtatgtgta atatcctcca acaactctgg cacctctgtt tctttaggca actgtcttaa
    18481 caaagagcca aaggactctt ccatatcagc tagttgtttt ttgtagacat ctaggttatt
    18541 tgccttaaaa actttttgct caaactcctg caacaaagtt tcctgagctt gctcaccaga
    18601 agcataagtt tcacgcatag gggaaagatc aaatacatac cccaaaacca acactagtat
    18661 aaagacgata atatacacta gtatttttgc cgctaaaggc catgaaccaa cattttgagg
    18721 atcaagcgta tttagagtat ttataaactc ctgcaaatca aattttttta cgtctgattg
    18781 agcttcagct ttgtcagttt ttaacttcat ttagctgcct ccttgttagt tttagcaaca
    18841 cccgttttat catctgtctt tattggttca ggcccttcca actcaacagt aagagcaaaa
    18901 cggttacttc ttttatccaa actaaccttt gtagctttct taacggtatc atctgctgtt
    18961 accgtggata aaacaggatt tttgaaccat tctgaagcat tcaaatttct caaaaaagaa
    19021 gacacctgtg tattgctttc agcattacct tcaatcgtaa atttatcacc atctcgtttg
    19081 acagatagca aataaacttt attaggcaca ctacggacta actcatcaaa aactctaact
    19141 ataatcggtc tacgcccttg caaatcttga atgactttca ttcgcgctaa taattcttct
    19201 tttcgcgttt gcaattcatc aatctgctta atttgtgagt ccaacatagc aatttctgat
    19261 tgaatataag cctttctttc ttcttgatca ctaagctgcg cactcaacat catatgaaaa
    19321 aagagaacaa ttgcggcggc ggcaagtgca actcctgcgt taattgcaac aaactcttgc
    19381 tgacgttgtt ttttaagctc ttgtcgccat ggaagtaagt ttatcttcgc catcagtcaa
    19441 aactccttaa tgctaagcca catgcaatca ttaatgaagg tgcatcatta ctaatcgccg
    19501 cagcattaat ttgaggagca aaagacatat gaacaaaagg atttgccaca gagactgccg
    19561 ttcctaattt ttcctgaaca acatcagcta agcctccaat agatgctgtg ccacctgcta
    19621 ataatatatg atcaacatca ttatattgac ttgaagaaaa gaaaa
//
LOCUS       DAOQMB010000007        17638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1131_length_17638_cov_16.543934, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000007 DAOQMB010000000
VERSION     DAOQMB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17638
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1131_length_17638_cov_16.543934"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PLY05_00690"
     CDS             <1..970
                     /locus_tag="PLY05_00690"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HNC01847.1"
                     /translation="AQIYNAILPANSQQLQAAMINMSPALYAESAQSVLALQQKLHNS
                     QTISETFKQGGLALRGFQQDTDVDSDGNGLAATRSISGVQLSVDSEPYANDWQMGAAI
                     SIVNKADVESNSSALKLSGHDVALTVRKKVSDWMLGATVDMGNYTFDTNRNINIVGTQ
                     FKTNQQDVKTSSYGVGVQASRNYGSWTLATGLRYNNVKQDGFVEQGNSLLALNVADVE
                     QDQVVAMLGGAWQNTWKTQMWNIVPKLSLDLEQVVAGDTAQAKAMLGAGQVTATASDA
                     GKTLFRAGVGVNFVNKDGITIGVDASTEQADNLSANTGRLTFSKSF"
     gene            1067..2536
                     /gene="rng"
                     /locus_tag="PLY05_00695"
     CDS             1067..2536
                     /gene="rng"
                     /locus_tag="PLY05_00695"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HNC01848.1"
                     /translation="MSEEVLINITPMECRVALVENGIVQELFVERTAKRGLVSNIYKG
                     KVVRVLPGMQAAFVEIGLSRTAFLHANDIVHLKKELMLEPPPITDLVHEGQMVTVQVI
                     KDMLGSKGARLTTDLSIPSRYLVYIPFGEHIGISQRIESEEERERLKAVIRHIRDEHS
                     DSLKGGVIVRTAAEGISEEELRQDMAYLIKLWQHINDKKQKVQNPTLIYEELPLYQRI
                     VRDLVIQNISKIYVDSRETFGKLVDFVKEFVPNAEEKLEHYPGERPLFELYNIEDDLQ
                     KALSRKVALKSGGYLIIDQTEAMTTVDVNTGSFVGSRTLEDTVFKTNLEATHAIARQL
                     RLRNLGGIIIIDFIDMQEEEHKSQVLKAFEKMLARDHAKTKITQVSELGLVEMTRKRT
                     RESLEHLLCEVCPTCQGRGSIKTAETVCFEIFREILREARAYDAANGYMVIANQKIID
                     RLLSEESAAVADLELFIAKPIRFQVESLYTQEQYDIVLL"
     assembly_gap    2693..2792
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2813..6682
                     /locus_tag="PLY05_00700"
     CDS             2813..6682
                     /locus_tag="PLY05_00700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HNC01849.1"
                     /translation="MLLRSSKPRWQRSQHWFNKLSKGMIYIISGLTMTTLIIKGTFVY
                     LESHQQVIEQQLSAAMTTTVKIGEFNAQWTGIEPELRLSNIRIYHPQNPHQEILVIPK
                     ITLEIAVWRTIREADLRLDGIIEGLDLHITEFEQGAWALEELLALGESRPEVRKRSLT
                     WALQQAEWQVTKSQVMLKPWGKPILNLSNLNIHNQNNSEFHGFRLNAQLNQQPIKLFA
                     NIWAKDLFDAKTWRMQNYVNLPTMSWLAWLDQSLLPNNLQITEVLAGGEYWLETKNSQ
                     IKHIAAKPNIVSLKLNYQQQVIDVENLTGNLVWQQLANQSWQANTDNIVGKINQINWP
                     LKQSIVQKAPSTINIVANHIELKPLALIAQILPQLPLNIKQWLSEAKPSGQIDHLSLQ
                     LDQQQNIQQAAASFKQLAILATLKTIGINRAQGWLTHQQGKGYAQLSIEQGGIDLKPI
                     YRQPTPLNHLQAALRWQELPEAWLVESNKITLKNPDAHGDATLRLWIPKADPSATEMT
                     LLASIYNGKLASVYRYVPWPSAGDDTLTWLKSALVAGNIERGDFLYEGVLIDKPTRRP
                     SNMQMRFWVNDGQLAYATDWPWLNQLNAQIDIYNRKLTIQAKNSQIYQSIAREIYAEI
                     PDLSQPHLRIKADIDTTGEDIMRLLSETPLKEETGHFAQMVGVNGEVAGNLELNFPLA
                     SDHLAKETTVDVMAELVGNPIILKQAPEFDLWLSGGVNYKTGFGLNSLPLQGFFLSQP
                     VNVSLQSVLDVGNVAAIQVKATGQLAPTNLKPWLGDLTRSMQGLTQYKTTLTVPVSDD
                     PVHIVIDSDLLGWQIDLPEPFAKQANKTQAAHYEMELHSEQKQSAYLMWGESLQSAFE
                     IENSAIKRMLLLLGETWTGELPPQGLWVKGRLSHVNIDDWLPWWRPASHNKTPEKVAA
                     VFPELVSFNVEVDKLAYLGYVLKNARVGYEPVDEATRFQLTSNDLNAELIWPTNHKNQ
                     NAKFNIQQINLPFIADELTRKTPKSFKISDDWQIPNIDINIKNIRLKSRPHFANSQIT
                     AQLNPTAKGLRLANINCNNPKFNMEGTLDWRWRGQESTSYSGQINIANVADLFTAFGQ
                     EPRVTSQQAKSQLNLRWAGSPRDLHLNNLNGELLVILDKGRILNLNRTLSVSRLLGVL
                     DSDNFKRRLQFDFSDVTQKGLAYDKIRFDANILNGLMENELDFSSPSLQAHGEGAVDL
                     AKSKIDERITISAPLASAVPYAAALVAGPVVGGALVAAEAALDEPISKMTTLHYQVQG
                     SWQDPKVQRVHNPDLPWRKWFKKKTRK"
     gene            6683..7510
                     /locus_tag="PLY05_00705"
     CDS             6683..7510
                     /locus_tag="PLY05_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNC01850.1"
                     /translation="MTKCIVAALQMQSQDNLEDNLRQAAQLMEQAQQAGVQLVVLPEN
                     FAMFAAGKQLETAQNLAKIQQWLSQQAQQQQMWIVAGSIPCATRPDGTPVADNKVRAS
                     CLIYSPQGQLFGRYDKIHLFDANVADQQASYQESATFEAGSEVVIVETPFAKIGISIC
                     YDLRFPLLYQALRDKGAEIIVVPAAFTALTGEAHWQVLLRARAIETQSFVIGAGQSGW
                     HSATRQTWGHSQVIDPWGKVLAEQQQTGSGLIVAVCDLSELHLIRQQMPILRHRRLF"
     assembly_gap    7572..7671
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7840..8304
                     /locus_tag="PLY05_00710"
     CDS             7840..8304
                     /locus_tag="PLY05_00710"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC01851.1"
                     /translation="MSESLADYLGQVPIFSNLTLPELQTIEKYMFFNKVEVGEFVFKE
                     GEKGDYVCFVMHGALDVIKLNQHAKPVVITTLAKGSSIGEMALIDKLTRSASVRACTT
                     SGLIVLTRKGFEMILKLHPEIGIKILKGIASLLSINLRKTSDKLAEFMPPLA"
     gene            8362..>9488
                     /locus_tag="PLY05_00715"
     CDS             8362..>9488
                     /locus_tag="PLY05_00715"
                     /inference="COORDINATES: protein motif:HMM:NF025059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNC01852.1"
                     /translation="MSENSISDLLLAVEQAFQDHQLIKLVLSKYAGSETNLQRIDIKM
                     VSLRETLQLSFLYHYKTQDVTKNYSWIEAKSILTEFLGRQFKQANLLTTQQEWQLLIS
                     KKGKLSLTVKKQTQAISKNLPVEHNRQKHRYLSLERAFWPFLGITDQQRQLIPAMSRK
                     WSQINKFIEIFDNAISEAKLDLTQALNVVDFGSGKGYLTFAVYDYLREHYQNQATVTG
                     VELRDNLVALCNKAVNACDLQGLQFYQGDVRSYQPERLDVMIALHACDIATDYAIHTG
                     IRLQAKVIMCAPCCHKQLRPQMLCPTILKPVLQHGVHLGQEAEMITDGLRALLLEAEG
                     YQTQVFEFVSLEHTSKNKMILAIKKSISPQKKAEILTQIAE"
     assembly_gap    9489..9588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9589..10736)
                     /locus_tag="PLY05_00720"
     CDS             complement(<9589..10736)
                     /locus_tag="PLY05_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC01853.1"
                     /translation="MTIMNRNHRPYDIILLGATGFTGQLTADYLIKHAPQIKLALAGR
                     NAEKLQHIREVLLADNPNAPMPDLFVADSHNMASLKQLCSQTRTVISTVGPYAVHGED
                     LVAACITQQCDYVDLTGEPQFVYKMQEKYAADATKAKVKIVHCCGFDSIPHDLGVLFT
                     LNALAQKLGHDVLAQSSVSVEGFVRGHGDISGGTWNSAISAMPDWQNFVKQRQAQYKQ
                     YYQGRDIRLIEKPTLRKLKDGWAAPLPTIDPAIVVQSARRYSHYGQKFSYAHFGVVPQ
                     LHRLAMTAAAVAGIFTLAQLEKGRTWLAKQRPSGTGPTPEDRSTHWFNVEFCASVETP
                     QQQNVTLHTLVSGGDPGYDETAKMLAESALCLLLDKDALPAHFGIVTPA"
     assembly_gap    10958..11057
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11058..11266)
                     /locus_tag="PLY05_00725"
     CDS             complement(<11058..11266)
                     /locus_tag="PLY05_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01854.1"
                     /translation="MLKIKSLTILLLLSSSLYANDHDVDFGFDLDGKYQQQNYKNATS
                     KSYRLGLQPWLLIDNWLFYAELPFEA"
     gene            complement(11394..11879)
                     /locus_tag="PLY05_00730"
     CDS             complement(11394..11879)
                     /locus_tag="PLY05_00730"
                     /inference="COORDINATES: protein motif:HMM:NF022748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3106 domain-containing protein"
                     /protein_id="HNC01855.1"
                     /translation="MKRFLIISLLLISASSWAEGVRWQDLPPASQEALMNLKDNWDKL
                     PADKQSAIAKRIDHWQSLSDEDKNEAKQRFERWQQLSPEKRQKLLAKYQEFAQLPLDE
                     QEEIKRKWRWFKKLPPEQREIMRERWQSLSIEERRALRERLRQANPEQRRELRRDFLR
                     R"
     gene            complement(11876..12106)
                     /locus_tag="PLY05_00735"
     CDS             complement(11876..12106)
                     /locus_tag="PLY05_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01856.1"
                     /translation="MKKWQTSLLLSCLLTPVAYAQPALDPAFINYLLEFGEQPELFDA
                     AHQELESNTHNAPTQSNNQRQQSANNTKEIKQ"
     gene            complement(12103..12453)
                     /locus_tag="PLY05_00740"
     CDS             complement(12103..12453)
                     /locus_tag="PLY05_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01857.1"
                     /translation="MSHDTRKDEMMVAHICQSLDQHSQQAHEFDDKLQLLAEQAQRQR
                     HQHLQNRQQQKKWWYLGGSLAIAATVTMVIFSPKLIQQSTHSQQTKAVAIHTSVDPQL
                     LEDMDMLMVLGEEQ"
     gene            complement(12450..13019)
                     /locus_tag="PLY05_00745"
     CDS             complement(12450..13019)
                     /locus_tag="PLY05_00745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006550.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNC01858.1"
                     /translation="MSTRPAPLTIQQWLKEIGRRALVMTELATNFHHQSQDIVQDSLL
                     SFINHYSDKPSEQWTPLFYGILRNQITDWKRKQARRSKWLTWFSSHTIDDEDEINPFE
                     QIANSYEDNPAQLLANASDIKLVQQVLSTLPERQQQAFLLRAWEGLDIQTTSQIMGCS
                     ESSVKTHYSRALSILRSALNQANVGGETS"
     gene            complement(13248..13835)
                     /locus_tag="PLY05_00750"
     CDS             complement(13248..13835)
                     /locus_tag="PLY05_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01859.1"
                     /translation="MTKGHALILAAVLATITQQTFAREGGFGGGQRMEHARPHQGEMG
                     REGAAKRQNFSETSTRTLANGETVKRETTQTVTDNGFTRNSTLTNGAGQTATKNLTVV
                     NDKETGTHTRTMSGTTFDGKSYSNTSTSQKTEDGFNRSHSFTTPDGKTGSKQVTGVVD
                     KEAGTVTKTTTINTPNGESKQHSVTHQLNPQTSAN"
     gene            13994..14464
                     /locus_tag="PLY05_00755"
     CDS             13994..14464
                     /locus_tag="PLY05_00755"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HNC01860.1"
                     /translation="MLNIVLFRPEIAGNTGNIIRLCANTGANLHLVKPLGFDLEDSKL
                     RRAGLDYHEWAMVQIHDNWSACLQHIQPKRIFAFSARAKQNAYSVAYNEQDLLLFGPE
                     STGLTPEMMTELNLHAEQFVKLPMKAGRSLNLSNAVAVTVYEAWRQCHFCGATE"
     gene            complement(14440..15012)
                     /locus_tag="PLY05_00760"
     CDS             complement(14440..15012)
                     /locus_tag="PLY05_00760"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC01861.1"
                     /translation="MQNFFGLIDRLNAHPEQKDELETQIWQQYGFHKAVLALDMSGFS
                     LTVRRQGIIAYLAKIRKMQHCTQPLISQHNGELVKYSADNLMATFDDCQQALLAAIDI
                     SQQCQQQDTPVSIGLDFGRILTIPQQDCYGDAVNIAYKLGEDLAEKNEILVSDNFYQQ
                     ITPHTTVQFESHQFHISGLEFKAYSVAPQK"
     gene            15165..>17638
                     /gene="aceE"
                     /locus_tag="PLY05_00765"
     CDS             15165..>17638
                     /gene="aceE"
                     /locus_tag="PLY05_00765"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HNC01862.1"
                     /translation="MTQHKIYNDIDSVETQEWLEAFESVVEREGVERAKFLLQALTAH
                     ANRFGVQLSRLNTPYINTISPQEEPMLAGDPYMERRIRSLIRWNALAMVMRANDNDDE
                     LGGHIATFASSATLYDIGFNHFFRAPHDTFGGDLIYYQGHSSPGIYARSYLEGRISEA
                     QLENFRREVDGKGLSSYPHPWLMPDYWQFPTVSMGLGPIMAIYQAHVTKYLINRELLP
                     EQNRKVWAFLGDGEMDEPESLGAIALAGREKLDNLVFVINCNLQRLDGPVRGNGKIIQ
                     ELESVFRGAGWNVIKVVWGRKWDNLLANDTTGVLKQRMEECVDGEYQAFKTHGGAYTR
                     EHFFGKYPELKAMVEHLTDEEIYQLNRGGHDPYKVYAAYAKAMAHQGQPTVILAKTVK
                     GYGTGAGEAVNKTHQLKKLDLDSLKAFRDRFDMPFSDEELKKVPFYRPSENSLELRYM
                     HERRQKLGGYLPSRDNQAHPLVVPELAQFDSVLQGSERAISTTMAFVRLLNILLKDSQ
                     IGERVVPIVPDEARTFGMEGMFRQMGIYSHVGQLYTPEDSAQMMYYKEDKKGRMLEEG
                     INEAGAMSAWIAVGTSYCMHRLPMIPFYIFYSMFGFQRIGDLAWAAGDIQAKGFLLGA
                     TSGRTTLNGEGLQHQDGHSHLLASTIPNCVAYDPCYAYELAVIIQDGLRRMYQQNESV
                     FYYLTTMNENYPHPAMPSGVEEGIRRGLYLLKAAPEGHGHQVQLLGSGVILREVEAAA
                     ELLMNVYGIRAAVWSVTSFNELRKEALACEHWNHLHPLEAPKVPWVTQQLHAVKGAII
                     AASDYMRIVSEQIRAFLPQGLPYITLG"
BASE COUNT         5286 a         2941 c         3692 g         5319 t
ORIGIN      
        1 tgctcaaatt tataatgcaa tattgcctgc aaatagccag cagttacaag cagcgatgat
       61 caatatgagt cctgctttat atgctgagtc agcacaaagc gtcttggctt tacagcaaaa
      121 actccataat agtcagacca ttagtgaaac gtttaaacaa ggtggtttag ccttacgtgg
      181 attccaacaa gatactgatg ttgatagtga tggaaacggc ttggcggcaa cacgtagtat
      241 ttcaggtgta cagctatccg ttgatagcga accctatgct aatgattggc aaatgggagc
      301 tgctatttcg attgtcaaca aggcagatgt tgaatctaat agttctgcac tgaagttatc
      361 aggtcatgac gtggcattga ctgtacgtaa aaaagtttct gattggatgt tgggtgcaac
      421 cgttgatatg ggtaactata catttgatac taatcgtaat atcaatattg taggtacaca
      481 gtttaagacc aatcagcaag acgtgaagac aagcagttat ggtgtaggtg ttcaagcaag
      541 ccgcaattat ggttcatgga cattggcaac tggtttacgc tataacaacg tgaaacaaga
      601 tggttttgtt gaacaaggta atagcctttt agctctcaat gtggctgatg ttgaacaaga
      661 tcaagttgtg gcaatgttag gtggtgcatg gcaaaacact tggaaaactc aaatgtggaa
      721 catcgtacct aagttaagtt tagacttaga acaagttgtt gcaggtgata ctgcacaagc
      781 taaagcgatg ctgggtgctg gccaagtaac agccactgct tctgatgcag gtaaaacttt
      841 atttagagca ggtgtaggcg ttaactttgt taataaagac ggtattacaa ttggtgtaga
      901 tgcctctact gaacaagcgg ataatttatc agcaaataca ggtcgattaa catttagcaa
      961 atcgttctaa catttagcag tctataaaat taaggccgac tctttgtcat ttggtgttaa
     1021 aaccaaatta acattagtcg gcctttgtta tcctgagcaa taaaaaatgt cagaagaagt
     1081 tttaattaat attacgccaa tggagtgtcg agtagcatta gtcgaaaatg ggattgttca
     1141 agaattattt gttgaaagaa cagcgaagcg aggtctagtc agtaatattt ataaaggtaa
     1201 ggtagtgcgt gttttaccgg gtatgcaggc tgcctttgta gaaattggtt tatctagaac
     1261 agcctttttg catgcaaatg atattgtcca tctaaaaaaa gaattaatgc tagagccgcc
     1321 cccgataacc gatttagtac atgaaggaca aatggtgacg gtacaagtca tcaaagatat
     1381 gctcggtagt aaaggtgctc gtttaacaac agacttatct attccttcac gttatttagt
     1441 gtatatacca tttggtgaac atattggtat ttctcaacgt attgagtctg aggaagagag
     1501 agaacgttta aaagcggtga ttcgtcacat tagagatgaa cacagtgata gtttaaaagg
     1561 aggtgtgatt gttagaacgg cagcagaggg aatttctgaa gaagagctgc gtcaagatat
     1621 ggcttatttg attaagttat ggcagcacat caatgataaa aaacaaaaag tgcaaaatcc
     1681 aacacttatt tatgaagagt taccgctata tcaacgtatt gttcgtgatt tagtgattca
     1741 aaatattagt aagatttatg tagattcgcg cgagactttt ggtaagttgg ttgattttgt
     1801 taaagaattt gtgccaaatg ccgaagaaaa gttagagcat tatcctggtg aacgaccatt
     1861 atttgaattg tataacattg aagatgattt gcaaaaagcc ttatcacgca aagtcgcttt
     1921 aaaatcaggt ggatatttga ttattgatca aactgaagcg atgacaacag ttgatgtgaa
     1981 tacggggtct tttgttggct cgagaacttt agaagatacg gtgtttaaaa ccaatttaga
     2041 agcgacacat gctattgctc gtcaacttcg gctgcgcaat ttgggtggga ttattattat
     2101 cgattttatc gatatgcaag aagaagagca taaaagccaa gttttaaaag cctttgaaaa
     2161 aatgttagct cgtgaccatg caaaaaccaa aatcactcaa gtatctgagt taggtttggt
     2221 ggagatgact cgtaagcgta cccgtgaatc tttagagcat ttattgtgtg aggtttgtcc
     2281 aacatgtcag gggcgaggtt ctattaaaac agcagagaca gtatgttttg agatttttcg
     2341 tgaaatatta cgtgaagcaa gagcatacga tgcagcaaat ggttatatgg tcatcgccaa
     2401 ccaaaaaatt atcgatagac tactctcgga agagtctgcg gcagtggcag atttggaact
     2461 atttattgct aagccgattc gttttcaggt agaaagttta tatacccaag agcaatatga
     2521 cattgtcttg ttgtagagaa acatgactga tgtattttgc caaaattagc gcgttttttg
     2581 aactaataat cgttgaaggc gattttttgc tgagaaaata agcgataact gcattagtcc
     2641 taagaaggtg tagataattg ttattaaggt cttctaaacc acgttggcaa cgnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntcctaaga aggtgtagat aattgttatt
     2821 aaggtcttct aaaccacgtt ggcaacgcag tcagcattgg ttcaataagt taagcaaagg
     2881 catgatttat atcatttcag gcttaaccat gacgacttta atcattaaag gtacatttgt
     2941 gtatttggaa agtcatcagc aagttataga gcagcaactc tcggcggcga tgaccactac
     3001 agtgaagatt ggtgagttta atgcccaatg gacaggcata gaaccagaat tacgtttatc
     3061 aaatattcga atttatcatc cacaaaatcc ccatcaagaa attttggtta tacctaaaat
     3121 taccttagaa atagcggttt ggcgtactat cagagaagct gatttgcgtt tggatggaat
     3181 cattgaaggt ttagatttac atattacaga gtttgagcaa ggtgcatggg cattagagga
     3241 attattagct ttaggtgagt cgcgtcctga agtgagaaaa cgtagtttaa cgtgggcttt
     3301 gcaacaagca gaatggcaag tcactaaaag tcaagttatg cttaaaccgt ggggtaagcc
     3361 gatcttaaat ctaagcaatt taaatattca taatcaaaat aatagtgaat ttcatggttt
     3421 tagattaaat gcacaattaa atcagcaacc tattaaacta tttgccaata tttgggcaaa
     3481 ggatttattt gatgccaaaa cgtggcgtat gcaaaattat gtcaatttac caacgatgtc
     3541 atggttagct tggttagatc agtcactgtt accaaataat ttacaaataa ctgaagtgtt
     3601 ggctggaggc gaatattggt tagagacaaa aaatagtcaa ataaaacata tagcagctaa
     3661 acctaatatt gtttcactta aattaaatta tcaacaacaa gtcatagatg tagaaaatct
     3721 aacaggtaat cttgtttggc agcagttggc taatcagtcg tggcaagcta ataccgataa
     3781 tattgttggt aaaattaatc aaataaattg gccgttaaaa caaagtattg tgcaaaaagc
     3841 tccatcaaca ataaatatag ttgccaatca tattgaatta aaaccattgg ctttaatcgc
     3901 acaaatattg ccacaattgc cattaaacat aaaacaatgg ttgagtgaag ctaaaccttc
     3961 aggtcaaata gatcatttaa gtttacaact cgatcaacaa caaaatattc aacaagcagc
     4021 ggcaagtttt aaacaattag caatattagc aacgttaaaa acgataggta ttaatcgtgc
     4081 gcaaggatgg ttaacccatc aacagggcaa aggatatgct caactgagta ttgaacaagg
     4141 tggtattgat ttaaaaccta tttatcgaca gccaacacca ttaaatcatt tacaggcagc
     4201 acttcgttgg caagaacttc ccgaagcgtg gcttgttgaa agcaataaaa taaccttaaa
     4261 aaatccagat gcgcatgggg atgctacttt acgtttatgg atacctaaag ctgatccatc
     4321 tgcaacagaa atgacacttc tagcaagtat ttataatgga aaattagcaa gtgtttatcg
     4381 ttatgtccca tggccatcgg caggtgatga caccttaaca tggttaaaat cagcgttagt
     4441 tgcaggcaat attgagcgtg gtgatttttt atatgaaggc gtattgattg ataagccaac
     4501 acgtcgtcct agtaatatgc aaatgcgttt ttgggtaaac gatggtcaat tggcttatgc
     4561 cactgattgg ccgtggttaa atcaattaaa tgcacaaata gatatttaca atcgaaaact
     4621 cactatacaa gctaaaaata gtcaaattta tcaaagtatc gcgcgtgaaa tttatgctga
     4681 aatacctgat ttaagtcagc cacatttgcg tattaaagca gatattgata ctactggcga
     4741 agatataatg aggttattaa gcgaaacacc gcttaaagag gaaacaggtc actttgcaca
     4801 aatggtgggt gtcaacggtg aagttgcagg caatttagaa ttaaattttc cattggccag
     4861 cgatcatttg gccaaagaaa ccactgtgga cgtaatggcc gaattagtgg gtaatccaat
     4921 tattttgaaa caggcaccag agtttgattt atggttaagt ggaggggtga attataaaac
     4981 gggatttggt ttaaatagtt tgccattaca aggatttttt ctgagccaac ctgtgaatgt
     5041 tagtctgcaa agtgtcctag atgtaggtaa tgttgccgct attcaagtga aagcaactgg
     5101 tcaacttgcc cctaccaatt taaaaccttg gttaggtgat ttaacgaggt cgatgcaagg
     5161 tttaactcaa tataaaacga ccttaaccgt tcctgtcagt gatgatcctg tgcatattgt
     5221 cattgatagt gatttgttag gttggcagat tgatttgcca gaaccttttg ctaaacaagc
     5281 caacaaaaca caagcggcac attacgaaat ggagctacat tctgaacaaa aacaatcggc
     5341 ttatttgatg tggggggaat ccttacaatc agcatttgaa attgaaaact cggccattaa
     5401 gcgtatgtta ttgttgttag gggaaacttg gacaggtgag cttcctcctc aaggtttgtg
     5461 ggtcaaagga cgtttgagtc atgtcaatat tgacgattgg ttaccttggt ggcgacctgc
     5521 aagccacaat aaaacacccg aaaaagttgc cgctgtgttt cctgaattgg tcagttttaa
     5581 tgtggaagta gataagcttg cttatttggg atatgtgtta aaaaatgcac gagttggcta
     5641 tgagcctgta gatgaggcaa cgcgatttca actaactagt aatgacttaa atgccgagtt
     5701 aatttggcca actaatcata aaaatcaaaa cgctaaattt aatattcaac aaattaattt
     5761 gccgtttatt gccgatgaat taacccgtaa aacaccaaaa agtttcaaaa tcagtgatga
     5821 ttggcagatt cccaatatcg atattaatat caaaaatatt cgactcaaat cgcgtccgca
     5881 ttttgccaat agtcaaatta cggcacaatt aaatcctaca gctaaaggct tacgtttagc
     5941 caatatcaat tgtaataatc ctaagtttaa tatggaaggg actttagatt ggcgttggcg
     6001 tggtcaagaa agtacgagtt atagtggcca aattaatatt gccaatgtcg cagatttgtt
     6061 tacggcattt ggtcaagagc caagagttac cagtcaacaa gcgaagtcgc aactcaattt
     6121 acgttgggca ggtagcccaa gagatttaca tcttaataat ttaaatggtg aattattagt
     6181 tattttagat aaaggtagaa ttctcaatct aaatcgtacc ttatcagtgt ctcggctatt
     6241 aggcgtatta gatagcgata actttaaacg tcgactacag tttgattttt ctgatgtgac
     6301 gcaaaaaggt ttagcctacg ataaaattcg ttttgatgcc aatattctca atggcttaat
     6361 ggagaatgaa ttagacttta gcagtccatc attacaagca catggtgaag gggcagttga
     6421 tttggctaaa agtaagattg atgagcgaat tactatttca gcacctttag cgagtgctgt
     6481 cccgtatgct gctgctttgg ttgcagggcc agtggtgggt ggtgctttag tggctgccga
     6541 agctgcacta gatgaaccaa ttagcaaaat gacaacatta cattatcaag tacaaggaag
     6601 ttggcaagac ccaaaagtac agcgagtaca caatcctgat ttaccttggc gtaaatggtt
     6661 taagaaaaaa acgagaaaat aaatgactaa atgtatagtt gcggcattac aaatgcaaag
     6721 ccaagataat ttggaggaca atttacgaca agctgcacaa ttaatggagc aagctcaaca
     6781 agctggtgta cagttagtcg ttttacccga aaactttgca atgtttgctg caggtaaaca
     6841 attagaaact gcacaaaact tagctaaaat tcaacaatgg ttgagtcaac aagcacaaca
     6901 acagcaaatg tggattgtcg caggtagcat tccttgtgca acgaggccag atggcacgcc
     6961 tgttgctgat aataaagtca gagcaagctg tttgatttat agcccacaag gacaattatt
     7021 tggccgttac gataaaatac atttatttga tgccaatgtt gccgatcaac aagcgagcta
     7081 tcaagaatca gcgacctttg aagcgggttc ggaggtagtt attgtcgaaa cgccttttgc
     7141 taaaattggc attagtattt gttatgattt gcgttttcct ctgttgtatc aagccctaag
     7201 agataaaggt gctgagatta tagtggtacc agcggctttt accgccttga caggtgaagc
     7261 acattggcaa gttttattaa gagcacgagc cattgagact cagtcttttg ttattggcgc
     7321 agggcaaagt ggctggcaca gtgcaacccg acaaacgtgg gggcattcac aagttattga
     7381 tccgtggggt aaggtattag cagaacaaca gcagacaggc agcggattga ttgttgctgt
     7441 ttgtgattta agcgaattac atcttattcg tcagcaaatg cctattttgc gacatagacg
     7501 actattttaa atacgcaagc tagcgtaaga ttgtgacatt aagtgacaca gattgtcagt
     7561 gtttatcgtc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngacacatga
     7681 attatgtaac aatagacaca acaagcctct ataaattcat gtataatatt tttaagtaat
     7741 tgaatgtttt atgctttaat tttagtgaac ataaaacttt ttaaagtata aagttaaatg
     7801 ctatgttgtg tggttaccgc atcaaaatgg agaacaataa tgagtgagag tttagctgat
     7861 tatttaggac aagtgcccat tttttctaac ctcaccttgc cagaattgca aactatcgaa
     7921 aaatatatgt tttttaacaa agtagaagtg ggtgagtttg tatttaaaga aggtgaaaag
     7981 ggcgattatg tctgttttgt aatgcatggt gctttggatg tgattaaatt aaatcaacat
     8041 gctaaacctg tggtgattac aaccttagct aaaggaagct caattggtga aatggcatta
     8101 attgataaat taactcgttc cgcatcagta cgagcttgta caacctcggg tttaattgtt
     8161 ttaacgcgca aaggttttga gatgatttta aaattacatc ccgaaattgg cattaaaatc
     8221 ttaaaaggaa ttgcgtcgtt attaagtatc aatttgcgta aaacctcgga taaattagct
     8281 gagtttatgc cgcctttagc ataatatttt agaagttttt taaaggcagc ttacgctgcc
     8341 tttatttttt agtgtgtgct tatgtctgaa aattctatta gtgatctatt gttagctgtt
     8401 gagcaagctt ttcaagatca tcaactcatc aaattagtat tgagtaaata tgcaggtagt
     8461 gaaaccaatt tacaacgtat tgatattaaa atggtgagtt tgcgcgaaac cttgcagtta
     8521 tcatttttat atcactacaa aacacaagat gtgactaaaa attacagttg gatagaggca
     8581 aaatctatct taactgagtt tttaggtaga cagtttaaac aagctaattt gttgactaca
     8641 caacaagaat ggcaattatt aatcagtaaa aaaggtaagt tatcattaac agtcaaaaaa
     8701 caaacacaag ccatttctaa aaatttgcca gtagaacata accgtcaaaa gcaccgttat
     8761 ttgagtttag agcgagcttt ttggccattt ttagggatta ccgatcagca gagacaatta
     8821 attcctgcta tgtctcgtaa atggtcacaa attaataaat ttatcgaaat ttttgataat
     8881 gcaattagtg aggctaaatt agatttaaca caagccttaa atgttgtcga ttttggttct
     8941 ggtaagggtt atttgacatt tgcggtgtat gattatttgc gagagcatta tcaaaatcaa
     9001 gcaacagtaa cgggcgtaga actgcgtgat aacttggttg ccttgtgtaa taaagccgta
     9061 aatgcctgtg atttgcaagg tttgcaattt tatcaagggg acgtgcgtag ttatcaacca
     9121 gaacgtttgg atgtgatgat tgctttacat gcttgtgata ttgcaaccga ttatgccatt
     9181 catacaggca ttagattgca agccaaagtc attatgtgtg cgccgtgctg tcataagcaa
     9241 ttaagaccac aaatgttgtg tccaacaatt ctcaagcctg tattgcaaca tggtgtacat
     9301 ttaggtcaag aagctgaaat gattactgat ggcttacgcg ctttattatt agaagcggaa
     9361 ggttatcaaa cgcaagtttt tgaatttgtt tctttggaac ataccagtaa aaataaaatg
     9421 atcttagcca ttaaaaagtc tattagtcct caaaaaaaag cagagatact gacacaaatt
     9481 gccgaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aggtgtcaca
     9601 ataccaaaat gtgcaggtaa agcatcttta tctaataata aacataaggc actttcggct
     9661 agcattttag cggtctcatc ataaccagga tcaccaccac tgactaaagt gtgtagtgtg
     9721 acattttgtt gttggggggt ttcaactgag gcacaaaact caacattaaa ccaatgtgtg
     9781 ctgcgatctt cgggggttgg gcctgtgcct gaagggcgtt gtttggcaag ccaagttcga
     9841 cctttttcta gttgagctaa agtaaaaata cctgcaactg cggcggcagt catggctaaa
     9901 cggtgtaatt gaggtacaac accaaaatga gcataactaa atttttgtcc ataatgactg
     9961 tagcgtcgcg cactttgcac tacaattgcg gggtcaatgg taggcaatgg tgcagcccag
    10021 ccatctttaa gtttgcgtag tgtcggtttt tcaattaatc gaatatcgcg tccttgatag
    10081 tattgtttgt attgagcttg tcgttgttta acaaagtttt gccaatcagg catagcactt
    10141 atagcggagt tccaagtgcc acctgaaatg tctccatgac cacgaacaaa tccttctaca
    10201 ctgactgatg attgcgctaa aacatcgtga cctaattttt gtgcaagtgc attgagtgtg
    10261 aataaaacac ctaagtcatg ggggatacta tcaaatccac aacaatgaac aattttaact
    10321 ttggctttag ttgcatcggc tgcatatttt tcttgcattt tataaacgaa ttgtggctca
    10381 cctgttaaat caacataatc acattgttga gtaatacaag ctgcaactaa atcttcacca
    10441 tgtacagcat aaggaccaac ggtactaatg acagtacgtg tttgtgagca taattgttta
    10501 agactggcca tgttatggct atctgccaca aataaatcgg gcataggtgc attaggatta
    10561 tccgctaata acacttcacg gatatgttga agtttttcgg cgttacgacc tgctaaggca
    10621 agtttaattt gtggagcgtg tttaattaaa taatcagcag ttaactggcc tgtaaagccc
    10681 gttgctccta ataggataat gtcataagga cgatggttac gattcataat agtcatttat
    10741 tgtctaagag aggagtgcta aatgactaat tttaacggta aatcacttaa aataaaaact
    10801 ttgcaccaac actaaactca ctttctggta aagaaatatg ttgttgtaag tattggccat
    10861 agctactgta gatgtttaat gcacgattga tacgccaatc gacacgtgat tgtataaaag
    10921 cctgttcagg tgcattgtgg taaggttcgg tttggttnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnngct tcaaaaggga gttcagcata aaacaaccaa ttatctatga
    11101 gtaaccaagg ttgcaaaccc aagcgataac ttttacttgt tgcattctta taattttgct
    11161 gctgatattt gccgtctaaa tcaaagccaa aatcgacatc atggtcattt gcatataaag
    11221 acgatgataa taatagaagt attgttagac ttttgatttt taacattatt attctctttt
    11281 aggacttatg cggttatcgc ttatttgttc gctaaataag cgttttcaac gattattagc
    11341 tcacaaaacg cgctaatttc ggtgaaatgc gtaagtcctg ttgttattaa aaattaacgg
    11401 cgcaaaaagt ctctgcgtaa ttcacggcgt tgttcgggat ttgcttgacg taaacgttca
    11461 cgtaatgctc gacgttcttc aatagataat gattgccatc tttcacgcat gatttcacgt
    11521 tgttctggtg gcaatttttt aaaccaacgc catttgcgtt taatttcttc ttgttcgtca
    11581 agaggcaatt gggcaaattc ttggtattta gcaagcaatt tttggcgttt ttcaggtgaa
    11641 agttgctgcc aacgctcaaa acgttgtttc gcctcatttt tatcttcgtc tgataagctt
    11701 tgccagtgat caatacgttt agcaatagct gattgcttat cagcaggtag cttatcccag
    11761 ttatctttta gattcatcaa agcttcttgc gaagctgggg gtaaatcttg ccaacgaaca
    11821 ccctctgccc agcttgatgc gctaattaag agtaaactga tgattaaaaa acgtttcatt
    11881 gtttaatctc cttggtgtta ttagcagact gttgacgttg attgtttgat tgagttggag
    11941 cattatgagt gtttgattct agctcctgat gtgccgcgtc aaataattcg ggttgttcac
    12001 caaactctaa taaataatta ataaaagcag ggtctaaagc gggttgtgca taagctacgg
    12061 gtgttaacaa gcaactcagt aataggcttg tttgccattt tttcattgtt cttctcctag
    12121 aaccatgagc atatccatat cttctaatag ttgggggtca acagaagtat gaatagcgac
    12181 tgcttttgtt tgttgagaat gagtgctttg ctgaattaat ttagggctga aaatgaccat
    12241 agttacggtg gccgcaatcg ctaagctgcc gcccaaatac caccattttt tttgttgttg
    12301 acgattttgt aagtgttggt gacgttgacg ttgggcttgt tctgccaaca attgtagttt
    12361 gtcatcaaat tcgtgagctt gttggctatg ttgatctagg ctttggcaaa tatgagcgac
    12421 catcatctca tctttgcgtg tgtcatggct catgatgttt ctccgccaac atttgcttga
    12481 tttaacgctg aacgcagtat gcttaatgct cggctataat gtgtttttac actactttcg
    12541 ctacagccca taatttgaga ggttgtttga atatctaaac cttcccatgc ccgcaataaa
    12601 aaagcttgtt gttgtcgttc tggtaaagtg cttaacacct gttgtactaa tttaatatca
    12661 cttgcattgg ctaagagttg tgcaggatta tcttcataac tattagcgat ttgctcaaag
    12721 ggattgattt catcctcatc atcaattgta tgactactaa accatgttaa ccatttacta
    12781 cgacgagctt gttttcgttt ccaatcagta atttggttgc gtagaatgcc ataaaataaa
    12841 ggtgtccatt gttcgctagg tttatcacta tagtgattga taaatgagag caaactatct
    12901 tgcacaatgt cttggctttg atgatgaaaa ttagtcgcta gctcggtcat tactaacgct
    12961 cggcgaccaa tttcttttag ccattgttgt atggttaatg gggctggacg tgtggacatt
    13021 gtattattct tggtttgaag atagccaata taatggctat cttttttcaa tactatgttt
    13081 ttaaatatag gacttacgca tttcaccgaa attagcgcgt tttgtgaact aaaaatcgct
    13141 aaacgcgatt gtttagtgag caaataagcg ataaccgcgt aagtcttaaa atagtccatg
    13201 accataagcc attgtgtgtc cataggcaag catcttttga cactctgtta atttgcactc
    13261 gtttgtggat tgagttgatg cgtgacacta tgttgtttgc tttcaccgtt aggcgtattg
    13321 atggtggtgg ttttggtgac cgtacctgct tctttatcaa caacaccagt cacttgcttg
    13381 ctacctgttt ttccatcagg tgtagtaaaa ctatgactac ggttaaagcc atcttctgtt
    13441 ttttggctgg tgctagtatt gctatagctt ttgccatcaa atgttgtacc tgacatggta
    13501 cgagtatgtg taccagtttc tttatcatta acaacagtga gatttttagt agcagtttgg
    13561 ccagcaccat ttgttaatgt actattacga gtaaaaccat tgtcagtgac tgtttgtgtg
    13621 gtttcacgtt tgactgtttc accattagct aaggtgcgag tgctagtttc actaaaattt
    13681 tgacgtttag cagcaccttc gcgtcccatc tcaccttgat gtggacgagc atgctccata
    13741 cgttggcctc caccaaatcc accttcgcga gcaaaagttt gttgagtgat agtggctaat
    13801 acagcggcta aaattaaagc gtgacctttt gtcatgatcg ttctccagta tttgagttga
    13861 acatacaaaa aagagctaag tatgttgcta ctgtaaaaaa cgtctgattt aaaataaagt
    13921 tgacaaaaaa aattaaaaat acataaaatt ttttctaata ttttttaagt ttataaatta
    13981 taagagaatt tttatgttga atattgtgtt gtttagacca gagattgcag gaaatacagg
    14041 aaatatcatt agattatgtg ctaatactgg tgcaaattta catctggtta aacccttagg
    14101 ttttgatttg gaagatagca aattgcgtcg tgcaggttta gattatcatg aatgggcaat
    14161 ggtacaaatt catgataatt ggtctgcctg tttgcaacat attcaaccca aacgtatttt
    14221 tgcttttagt gctagagcta agcaaaatgc ctattctgtt gcttataatg aacaagattt
    14281 attgttattt ggcccagaaa gtacaggttt aacgcctgag atgatgactg agttaaattt
    14341 gcatgcagag cagtttgtca aattgccaat gaaggcaggc cgtagtttga atttgtccaa
    14401 tgcggtagcg gtgactgttt atgaggcatg gcggcaatgt catttttgcg gagcaacaga
    14461 ataagcctta aattctaagc cagaaatatg aaactgatga gattcaaatt gcactgtcgt
    14521 atggggagta atttgttgat aaaaattatc gctgactaaa atttcatttt tttcggcaag
    14581 atcttcaccc aatttatagg caatattgac agcatcacca taacagtctt gttgtggaat
    14641 ggttaagata cgaccaaaat ctaaaccaat gctgacaggt gtatcttgtt gttgacattg
    14701 ttgactaatg tcaatggcgg ctaataaggc ttgttggcaa tcatcaaaag tagccatcag
    14761 attatcggca ctatatttaa ctaactctcc gttgtgttgg ctaattaatg gttgagtgca
    14821 gtgttgcatt tttcggattt tcgctaaata ggcaataatg ccttgacgac gcactgttaa
    14881 agaaaaccca ctcatatcta gagccaaaac cgctttatga aagccatact gttgccatat
    14941 ttgtgtttct agctcatctt tttgttcggg atgtgcatta agacgatcaa tcaggccaaa
    15001 gaaattttgc atgatttacc tacctccaat ttttaaacaa ttgcaatagc actgatttaa
    15061 gaatatcaca aacaggtgct taaaaaacaa actgacaaga ttgcgccccg agctgtttta
    15121 acggtataac cttgttaatc ataaaataaa acacggaggg gcgtatgact cagcataaaa
    15181 tatataatga tattgattcg gtagaaaccc aagaatggtt agaagctttt gaatcagtgg
    15241 ttgagcgcga aggtgtagag cgcgccaagt ttttattaca agccttaacc gctcatgcta
    15301 atcgttttgg tgttcagcta agtcgtttaa atacgcccta tattaatacc atttctcccc
    15361 aagaagaacc gatgttagca ggcgatcctt atatggagcg tcgaatccgc tcgttgattc
    15421 gttggaatgc tttggctatg gtgatgcgtg ccaatgataa tgatgacgag ttaggtgggc
    15481 atattgccac ctttgcttcg agtgctacgt tatatgatat tggttttaat catttctttc
    15541 gtgcaccgca tgacaccttt ggtggcgatt taatttatta ccaaggccat tcttcaccag
    15601 ggatttatgc ccgttcttat ttagaaggtc gaattagtga agcacaatta gaaaacttta
    15661 gacgtgaagt ggatggcaaa ggtttgtcga gttatccgca tccgtggtta atgcccgatt
    15721 attggcaatt tccaacagtg tcgatgggtt tagggccgat tatggccatt tatcaagctc
    15781 atgtgaccaa gtatttaatt aaccgagaat tgttacctga gcaaaatcgc aaagtgtggg
    15841 catttttggg tgatggcgaa atggatgagc cagaatcttt gggagctatt gccttagcag
    15901 gccgagaaaa actcgataat ttagtgtttg tcattaattg taatttacag cgtttagacg
    15961 ggccagtgcg cggtaatggc aaaattattc aagagttgga gtctgtattt agaggcgcag
    16021 gttggaatgt gattaaagtg gtgtgggggc gcaaatggga taatttgttg gccaacgata
    16081 ccacaggcgt tttaaagcaa cgtatggaag aatgtgtcga tggtgagtat caagccttta
    16141 aaactcacgg aggcgcatat acccgcgagc atttttttgg caaatatcct gaattaaaag
    16201 cgatggttga acatttaacg gatgaagaga tttatcagtt aaatcgtggt ggccatgacc
    16261 cgtataaagt gtatgccgct tatgccaaag cgatggcaca tcaaggccag ccaaccgtca
    16321 ttttggccaa aaccgtcaaa ggttatggta caggtgcagg cgaagcggtc aataaaacac
    16381 accaattaaa gaagctcgat ttagattcgc ttaaagcttt tcgtgatcgt tttgatatgc
    16441 cgtttagtga tgaagaactt aaaaaagtgc cgttttatcg tccttcagaa aatagcctag
    16501 aattacgcta tatgcacgag agacggcaaa aattaggcgg ttatttaccc agtcgagata
    16561 atcaagcgca tccattagtt gtgcctgaat tagcgcagtt tgacagtgta ttgcaaggtt
    16621 cagagcgcgc catttcaact accatggcat ttgtgcgttt gttaaatatt ttgctcaaag
    16681 acagccaaat aggtgagcga gttgtaccca ttgtgcctga tgaagcacgg acttttggta
    16741 tggaaggtat gtttagacaa atgggtattt attcccatgt tggtcagtta tacacacccg
    16801 aagactcagc gcaaatgatg tattacaagg aagataaaaa gggcagaatg ctcgaagaag
    16861 gcattaatga agcgggggca atgagtgcat ggattgcggt tggcacgtct tattgtatgc
    16921 accgtttgcc gatgataccg ttttatattt tttattcgat gtttggtttt caacgtattg
    16981 gtgatttggc atgggcggca ggtgatattc aagctaaagg ctttttgtta ggtgcaacca
    17041 gtggccgtac caccttaaat ggtgaaggtt tgcagcatca agatggtcat tcccatttat
    17101 tagccagtac cattcccaat tgtgtggcct atgacccttg ttatgcttat gaattagcgg
    17161 tgattattca agatggttta cgccgcatgt atcaacaaaa tgaatcggtg ttttattact
    17221 taacaaccat gaacgaaaat tatccgcatc ctgctatgcc aagtggtgta gaagagggta
    17281 ttcgtcgtgg tttatatctg ttaaaagccg cacccgaagg gcatggtcat caagtgcaat
    17341 tattgggttc gggcgtcatt ttacgcgaag tagaagcggc ggctgagtta ttaatgaatg
    17401 tctatggcat tcgtgctgcg gtttggagtg tgaccagttt taatgagtta cgcaaagaag
    17461 ctttagcttg tgaacattgg aatcatttgc atcctttaga agccccgaaa gtgccgtggg
    17521 tgacacagca acttcatgca gttaaaggcg caattatcgc tgccagtgat tatatgcgta
    17581 ttgtctcaga acaaattaga gcctttttac cccaaggttt accctatatc accttagg
//
LOCUS       DAOQMB010000008        17396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1159_length_17396_cov_14.502739, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000008 DAOQMB010000000
VERSION     DAOQMB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 17396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17396
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1159_length_17396_cov_14.502739"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..796
                     /locus_tag="PLY05_00770"
     CDS             2..796
                     /locus_tag="PLY05_00770"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC01863.1"
                     /translation="MNKYVSIENVAMRFETKKGTFEALRDINLGIEEGEFVSLIGHSG
                     CGKSTLLNLIAGLTLPSDGYLICNGREIAGPGPDRGVVFQNHSLLPWLTCFDNVYLAV
                     ERVFGDKETKKQLQERTHAALKLVGLTHATHKYPKEISGGMKQRVGIARALAMKPQVL
                     LMDEPFGALDALTRAHLQDELLKIVADTKATVVMVTHDVDEAVLLSDRIVMMTNGPSA
                     TIGDILTVDLAKPRDRMQLANDPHYHHLRSVVLEFLYKKHLRPAAA"
     gene            885..2102
                     /locus_tag="PLY05_00775"
     CDS             885..2102
                     /locus_tag="PLY05_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01864.1"
                     /translation="MKKLLVTAISLATIFPSLVLADAQELMTAIGGGKANMDIRLRTE
                     WVDFTTTKEATAATIRSTLAYKTADYQGFTGFIQFEDVTNFGKEDYNSTINGNAKYGI
                     IADPSLTQVNQIFIEAYGAKVGRQKIVYDNARFVGDVAWRQNDQTFDAISYVNKVLVP
                     STTFNLAYVNKVHNIVGKILPVSAPMLNVRYSHVAGDFGVNASAFYYGVEYDAVAATN
                     AAQNVSPAGSFQHIGTKVDGSFKDFLYEVSFAQQSEYADATSVGTPDANYMDLQLGYN
                     LGPVTVKVQQEVLEAGFKTPLATLHAFNGWADKFLATPVKGLEDTNLKLLAKYAGYNF
                     VLAGHSFKTDTDSIALGTELNFSVAKNINKNLSVMLKGAMYEADTDAKKLTTDAGLKT
                     DTKKGWVQAVYKF"
     gene            complement(2134..>3012)
                     /locus_tag="PLY05_00780"
     CDS             complement(2134..>3012)
                     /locus_tag="PLY05_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC01865.1"
                     /translation="QLSYSRLHEREADHVGIQTLTNAGFNPQAMADFFSKMDRQARQV
                     GLMPSFLLTHPSSQERNADSTLRSQQYPNKGKVDSLDYQLIRRRLLAIIDGRQADNLA
                     VLQQKLNNNPKHEPTLLSILFTLLNNEQFSQARQVATQLRTISPQRIDYIIAQADIEL
                     ADQQAQNAVKILEDALLINPQNQTLKMYAAKAAIKAQAYTQAIQWLSSLSYERANDPE
                     VWQSLVECYQAQKEGLQLLRARAEFAFLLGSYDKAISDLEQAKKLATNNYPLLAKIKQ
                     RQLVFEQTKEVSKKIK"
     assembly_gap    3013..3112
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3113..3411)
                     /locus_tag="PLY05_00785"
     CDS             complement(<3113..3411)
                     /locus_tag="PLY05_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01866.1"
                     /translation="MIRQFCQTSFILWNLFASSLLIADTNSESVLPDLGAFSVNKQQE
                     DQLGQQFLRELRASTAILSDPLIHDYLENLCYRLAFAAQSKHTNLDIVVLPDRNI"
     gene            3455..3676
                     /locus_tag="PLY05_00790"
     CDS             3455..3676
                     /locus_tag="PLY05_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNC01867.1"
                     /translation="MNQLDARGLRCPMPLLKLKQSLHQMNAGEVLTVMTTDPISQRDF
                     VVFLRQSPHQLLSMHQEQDVFYFQIEKAR"
     gene            3787..>4862
                     /gene="pheA"
                     /locus_tag="PLY05_00795"
     CDS             3787..>4862
                     /gene="pheA"
                     /locus_tag="PLY05_00795"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNC01868.1"
                     /translation="MTQHTLPTLPELRQKIDALDEQIQLLINQRAVCAQQVADVKKAE
                     DPTITSFYRPEREAQVLQMVMQRNQGPLAGETVARLFREIMSACLALESPLNIAFLGP
                     NGTFTHAAALKHFGHAAITQPISTIDEVFREVEAGSVNYGVVPVENSSEGVVNHTLDC
                     FMASSLKIIGEVELRIHQNLLVAQGTEIKNISRIYSHQQSLAQCRKWLDAHFPKVERV
                     AVSSNAEAARMVKNEWHSAAIAGETAAELYGLNILHANIEDNPNNTTRFLIIGTEEVP
                     ASGDDKTSLIIAAHNEAGALYHILEPFYRHNISLTRIETRPAQNDIWSYVFFIDFEGH
                     QQQPHVKVVLDEIKARVREMRILGS"
     assembly_gap    4863..4962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4963..5804
                     /gene="hisC"
                     /locus_tag="PLY05_00800"
     CDS             <4963..5804
                     /gene="hisC"
                     /locus_tag="PLY05_00800"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNC01869.1"
                     /translation="NCITLGNGSNDILELVARAYIAQGESAVYSQHAFAVYPLAVQAV
                     GGVGIEVPAKNFGHDLEAMAAAIQANTKVVFIANPNNPTGTWFERHEFEQFMAQVPAH
                     VLVVLDEAYVEYVTDAGFINGFDYVKQYANLIVSRTFSKAYGLASLRVGYAIASADVT
                     NILNRVRQPFNVNSFALAAATAALQDESFVKQSRELNSAGMAQLEQGLSSLALGFIPS
                     KGNFIAFDTGRPCLPVFQALLQQGVIVRPIANYGLPNYLRVSIGLESENARFLAALAQ
                     VLR"
     gene            5804..8011
                     /locus_tag="PLY05_00805"
     CDS             5804..8011
                     /locus_tag="PLY05_00805"
                     /EC_number="1.3.1.12"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional prephenate
                     dehydrogenase/3-phosphoshikimate
                     1-carboxyvinyltransferase"
                     /protein_id="HNC01870.1"
                     /translation="MFNQVTFIGLGLIGSSLARVIRAQGLAKKIVASSRSERTLQTAL
                     DIALIDEAYTDAAQAVANADVVVLAMPVCSTEKVLASIKHAVRPTTIITDVGSTKGNV
                     VDAARRVFGDVPSHFIPAHPIAGSEQSGVLAGKVDLFKGHKVIITPLAHSDKQATQQI
                     QQLWQAAGADVLTMTVERHDEVLAKTSHLPHFLAFSLVHALAKESQNLDIFRYAAGGF
                     RDFTRIAGSDPTMWHDIFLANKASILNALTSFNSGVEQLKAAVEAEDSEAILGILTRA
                     QAARHHFTHMLAGTAYQKTMTTKNLQFVLEAGGTLQGNLRVAGDKSISHRSIMLGSIA
                     EGVTEVSGFLEGEDALATLQAFRDMGVVIEGPKNGEVRIHGVGLHGLQAPPNAIYCGN
                     SGTSMRLLSGILAAQKFDTVMTGDPSLSKRPMERIAKPLRQMGAVIQTTGEKGTPPVS
                     IKGNQALQAMTYDMPVASAQVKSGILLAALWAEGETVVTEPAPTRDHSERMLRGFGYD
                     VKTEGSTIRIKGGGKLTATNIDVPADISSAAFFMVGASIAPNADVVLEHVGINPTRIG
                     VINILKAMGGDLTIMNEREVGGEPVADIRVRSAKLKGIHIDEADVPLAIDEFPALFIA
                     AACAEGTTILTGAEELRVKESDRIQVMADGLITLGIDAKPTPDGMIIQGGTMGGGKII
                     SHGDHRIAMSFAIASLRATGTIEIEDCANVATSFPNFVSLAKRAGLKIQVLEV"
     gene            complement(8066..9307)
                     /locus_tag="PLY05_00810"
     CDS             complement(8066..9307)
                     /locus_tag="PLY05_00810"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNC01871.1"
                     /translation="MINNDILQILATAFGLGLLVGLQREKAASALAGIRTFPLISLMG
                     AMTGLVAQHFGGWILASGFITLAIVILSAKFALFKSNQDLGITTEVAALLMYIVGAYL
                     TFGDATIAIVVGALTAVLLQMKDVLHDFVRKMGERDILAIMRFAAIALVILPILPNQN
                     YGPFHVLNPYDIWRMVVLIVAISLMGYAAYKVFKDKAGTLLAGILGGLISSTATTVSY
                     ARNTKTLPETSSLAALAIMLASTVSVIRVTLAFMVVSPSATSVIAPPLLTLFAIMVVI
                     CVALYFSRSGETHTMPEPDNPAELTSAIVFGLLYGGIIFAVAAAKEMFGQQSLYLIAM
                     ISGLTDVDAITLSTGRLVEGQRLEAHTGWQLVMIAIMANLVFKWGAVAVLGNKVLLKK
                     LTVIFGAAFMVGLGILAGWAV"
     gene            complement(9297..>10031)
                     /locus_tag="PLY05_00815"
     CDS             complement(9297..>10031)
                     /locus_tag="PLY05_00815"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002341.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HNC01872.1"
                     /translation="KLGGFAIMTASGLKFFTTPPHACSYLDNKQAITLFADPEAVMSN
                     PLYNQLSSYGFRRSGNYIYKPQCLQCQACISVRIPVHHFKQNRQQKRVWHKNQDLRVH
                     RIKAGYYQHHYDLYAHYIRSRHSDGDMYPPSVAQYLSFLFSDWSDTHLYEFWLEDRLV
                     AVATCDTLKNGLSAVYTFYDARLEKRSIGTYAILWQIEHTKQIGLDYLYLGYWVDGSP
                     KMAYKKNFFPLEALIDQVWRPLPNDK"
     assembly_gap    10032..10131
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10233..10979)
                     /gene="aat"
                     /locus_tag="PLY05_00820"
     CDS             complement(10233..10979)
                     /gene="aat"
                     /locus_tag="PLY05_00820"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNC01873.1"
                     /translation="MPVFLPPHSQQFPDIRYADESGLLAYGGDLTPETLLLAYQSGVF
                     PWYNESDVKSLLPWCNTPALWWSPDPRCVINPQLYQASHSLKKTLKSEKYSITVDRCF
                     AEVIRRCAAPRTYANETWINQKIIASYSKLHQMGVAHSIETWNQQGELVGGLYGINLG
                     KLFFGESMFSTATDASKVAFAFLMFICAKWQFPIVDCQLPNDHLLSLGAYTIPRTEFL
                     SILTDYKDLEKPNWQALQNVVHSTKEITLV"
     gene            complement(10982..11707)
                     /locus_tag="PLY05_00825"
     CDS             complement(10982..11707)
                     /locus_tag="PLY05_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNC01874.1"
                     /translation="MRSALVKFLTSLLSITSFYSYADDADNNNLWKGNIDVGYISSMG
                     STSGSKETFRGKTSASYNGTYWVQTVSAEGISVRDEVPATNDTERYLASYKARHFFGE
                     RNFFTLRTQWEKDLLSSSEYQAFISLGLGKEIIKTEKHFFKVEAGPGARHSEKRFAPA
                     KDEAIALLSLDYDWKITPNTKFLHKSTVEAGEDNTISRMQNQLKQNITKVIALTINHD
                     YKHDDGDKNTREGVFSLGLNYQF"
     gene            complement(11756..12832)
                     /locus_tag="PLY05_00830"
     CDS             complement(11756..12832)
                     /locus_tag="PLY05_00830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNC01875.1"
                     /translation="MTIEMQIEDVNVRSTEILITPAALKADIPLSDVAAKTVVEGRQT
                     IRNILDGKDHRLFLVVGPCSIHDINAAKEYAQRLKVLSEKVKDTLYLVMRVYFEKPRT
                     TVGWKGLINDPDMNDSFDIQKGLHIGRQLLLDLNEMGLPCATEALDPMSPQYMHDLIS
                     WSAIGARTTESQTHREMSSGLSSPVGFKNGTDGGLKVAINALLSVQNPHSFLGINSQG
                     QVAIINTTGNAYGHVVLRGGDGKPNYDSVSVTLAEQELAKSKIAPNIMVDCSHANSNK
                     KPELQPLVMDNVSNQILEGNKSIVGLMIESHLKFGRQDIPADLSQLEYGKSVTDGCIS
                     WDTTEEAILKMHDKLKEILPKRRA"
     gene            13110..13775
                     /locus_tag="PLY05_00835"
     CDS             13110..13775
                     /locus_tag="PLY05_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC01876.1"
                     /translation="MRSLAVAALAASVAVTGCTTNPYTGDKQASKAAWGAAIGALSGA
                     AVGAATGDNKKDRRERALKGAAIGGVVGGGVGAYMDAQEKKLREKLQSTGVGVKKNAD
                     GSIELIMPGNITFPTGQSAIKPDFFDTLNSVALVLKEYNKTSITVSGHTDNVGKDDLN
                     QKLSQDRANSVAQYLSSQGVDGGRISAVGKGKSEPIADNATENGRAQNRRVEITINPP
                     ESV"
     gene            complement(13816..15027)
                     /gene="opgC"
                     /locus_tag="PLY05_00840"
     CDS             complement(13816..15027)
                     /gene="opgC"
                     /locus_tag="PLY05_00840"
                     /inference="COORDINATES: protein motif:HMM:NF021633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HNC01877.1"
                     /translation="MSKNTERPPLSESGWHYPSAGRRDLRIDFLRGIAMIIMVVAHIE
                     VFSVFNLFTSERFGLVSGAEGFVIFAGIVLGSLYRHRLPIDGWITSIYRVWSRAWKLY
                     VVSLFIIISIYLLALLPFINAHEVISFTNRGINQVYPLYPVNDVPLNTALNFLFYLQA
                     GPHQTQVLGLYVILMLITPLAFVAFRYHKQWYFLAASWLVYFWYQKHVNIHITNSNFE
                     NAFAVLAWQALFFNALAIGWFKAEIASYMYGWRKILLLSFCVSVSLILFFIAQNHTNP
                     FLPSWAKLNLIEAHTFNYIYENYAQKNSLGVVRVLNDFCLLVTLYWLLTICWTPFYKA
                     LGWYLIPVGQASLYVFIIHVYVVLLASQLVSFNLSPNNNWLITSTIHGGSLMLLWWMT
                     KKEVGMKYIPN"
     gene            15090..16010
                     /locus_tag="PLY05_00845"
     CDS             15090..16010
                     /locus_tag="PLY05_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01878.1"
                     /translation="MRTAIHGRKFFLASFILLSTNTLEVKASEEDEQNPVWLGLSSSF
                     AFPYSQLFIKPSESEWDNLFKGSSIRLSYSHPLTKTVVAQGAGNQGETATNSTMQLGL
                     KYTPLSYWFVSANFTHYLQSDLQNTWDSDWSYSFGYDDWHPYTLSLTYSNSGGNSLSD
                     KKPRFNEGNWSLGWKFTIPETVKNIFVTGYGDNLGCGTNMSLTPRYIDSFTNQQASNK
                     VVLSMSCKYTIVGAWYINFSVLHYPQKEQQQAWNPDYTYGFGYFDWRPSKISVQYNNY
                     SGNRFNSAKRANGTGEFKNGSLSIAWSKSW"
     gene            16026..17267
                     /locus_tag="PLY05_00850"
     CDS             16026..17267
                     /locus_tag="PLY05_00850"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNC01879.1"
                     /translation="MEQASIIAEHLLPILIVDDSTSYRLLLMRHLKAWGEYEIIQAED
                     GIQALEIIKSRRVGMVISDWEMPNMNGLELCKAIRELSTNYVYFILVTSRGTSEDLIL
                     GMEAGADDFLAKPINQQELKVRVRAGERILRLEAHLEEKNQRLNATYQIMANDLKAAA
                     QMQQSLLPQGQLNIYNVNFDWFFKPSLFVSGDMLNYFRLDHENIGFYSVDVSGHGVKS
                     AMLSVTLSSLLNHNHTNSLVKKQIPDFPYYRISSPKEVVSTLNTQFQMTADNMIYFTI
                     VYGVLNVFTGQGMLTRAGHTSPIVIHANNVVEIIDSEGGVPVGFLEEPDYQEVAFSLE
                     NDSRLYLYTDGITECENKLSEQFGEQRLIDLLDKNHELPLQQTLAILQQELNLWRGGE
                     QEAFTDDVSMLTIHYVGNSLN"
BASE COUNT         5310 a         3145 c         3479 g         5162 t
ORIGIN      
        1 gatgaataag tacgttagca tcgaaaatgt cgccatgcgt tttgaaacca aaaaaggtac
       61 ttttgaagca ttgcgtgata ttaacttagg catagaagag ggcgagtttg tttccttaat
      121 tggtcattct ggttgtggta aatcaacctt attaaactta attgcgggct taaccttgcc
      181 tagcgacggt tatctaattt gtaatggccg agaaattgca gggccaggcc cagatcgtgg
      241 tgtagttttc caaaaccatt ctttattacc gtggttaacc tgttttgata atgtttattt
      301 agccgttgag cgtgtttttg gtgataaaga aacgaaaaaa caattacaag aacgtactca
      361 tgctgcctta aaactggttg gcttaaccca tgcaacgcat aaatatccaa aagaaatttc
      421 aggcggtatg aagcaacgtg tgggaattgc tcgtgcctta gccatgaagc cacaagtttt
      481 attaatggat gagccatttg gtgcgttgga tgccttaaca cgcgcccatt tacaagatga
      541 attattaaaa attgtcgctg ataccaaagc tactgtcgtg atggtaacgc acgatgttga
      601 tgaggccgtt ttattgtctg atcggattgt gatgatgacc aatggcccat ctgcaacgat
      661 tggcgacatt cttaccgtcg atttagccaa accgcgtgac cgtatgcagc tagctaacga
      721 cccacattat caccacttgc gctctgtagt cttagagttt ttatacaaaa aacatctacg
      781 tcctgctgcc gcataaaact taaatcctac ctattgtttt ttaattttag ggttgtctta
      841 acaaccctac gagtaagttt ttgcctaaat ttaggggtat taccatgaaa aagttattag
      901 taacagccat tagcttagca actatttttc caagtttagt attagcagat gctcaagaat
      961 taatgacggc cattggtggc ggtaaagcaa atatggatat tcgtttgcgt actgaatggg
     1021 ttgattttac gactaccaaa gaggcaaccg ctgccacgat tcgcagcaca ttagcttata
     1081 aaacggctga ttatcaaggg tttacaggtt ttattcaatt tgaagatgtg actaactttg
     1141 gcaaagaaga ttataacagt accattaatg gcaatgccaa atatggcatc attgccgacc
     1201 catcattgac tcaagtaaac caaatcttta ttgaagccta tggtgcaaaa gtaggtcgtc
     1261 aaaaaattgt ttatgataat gcccgttttg tgggtgatgt tgcttggcgg caaaatgacc
     1321 aaacttttga tgctattagt tatgtaaata aagtcttagt accaagcacc acatttaacc
     1381 ttgcttatgt aaacaaagtg cataacattg tgggcaaaat tcttccagtt tctgcaccga
     1441 tgttaaatgt gcgctatagc catgttgcag gtgattttgg tgttaatgcc agtgcctttt
     1501 attatggtgt tgaatacgat gcggtagcag caactaatgc ggcacaaaat gtgtctcctg
     1561 ctggctcttt tcagcatata ggcaccaaag ttgatggttc ttttaaggac tttttatacg
     1621 aagtgtcttt tgcccaacaa tctgaatatg cagatgcaac ttctgtaggg actccagatg
     1681 ccaattatat ggatttgcaa cttggttata acttagggcc agtcactgtt aaagtgcaac
     1741 aagaagtttt agaagctggt tttaaaacgc cactcgcgac cttacatgcg tttaatggtt
     1801 gggcagataa atttttggca actccagtca aaggtttaga agacaccaat ttaaagctat
     1861 tagctaaata cgcaggctat aactttgtat tggctggtca tagttttaaa acggataccg
     1921 atagtattgc tttaggtact gagctaaatt tttctgttgc taaaaacatc aacaaaaatt
     1981 tatctgtgat gttaaaaggc gcaatgtatg aagctgatac tgatgccaaa aaattgacaa
     2041 cagatgcagg gcttaaaacc gatactaaaa aaggttgggt acaagccgtt tataagtttt
     2101 aagcttggct aaataaaata gaggcgttag cctctatttt atttttttag atacttcttt
     2161 agtttgttca aaaaccaact gtcgttgttt aattttggct aataaaggat agttgttggt
     2221 tgcgagtttt ttggcctgtt ctaaatcact gattgcttta tcataactac ctaataaaaa
     2281 agcaaattcc gctcttgctc taagcagttg taaaccctct ttttgtgctt gataacattc
     2341 tactaagctt tgccaaactt cggggtcatt agcacgttca taacttaaac tgcttaacca
     2401 ctgaatcgcc tgtgtatagg cttgagcttt aatcgcagct ttagcggcat acattttgag
     2461 tgtttggttt tgtgggttaa ttaatagcgc atcttctaaa atttttactg cgttttgtgc
     2521 ttgttgatcg gcaagctcaa tatctgcttg agcaataata taatcaatac gttgtggtga
     2581 aatggtgcgt agttgtgtgg caacctgtcg agcttggcta aattgctcat tatttaacaa
     2641 ggtaaacaaa atagataata aggtgggttc gtgtttagga ttgttattaa gtttttgctg
     2701 taatacggca agattgtcag cttgtcggcc atcaataatc gctaataaac ggcggcgaat
     2761 taattgataa tccaaactat cgactttgcc tttgttcgga tattgttgac tgcgtagggt
     2821 gctatcggca ttacgttctt ggctagaagg atgcgttaat aaaaagctcg gcattaaacc
     2881 gacttgtcga gcttgacgat ccatcttgct aaaaaaatca gccattgctt gaggattaaa
     2941 gcctgcattg gttaaggttt gaataccaac atggtctgct tcacgttcat gtaaccgact
     3001 gtagctgagt tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttaatatt
     3121 tctatcaggt agcacgacaa tatcaagatt ggtatgttta ctttgtgcag caaacgctaa
     3181 gcgataacaa agattttcta aataatcatg aatcagtgga tcacttaaaa tagcggtact
     3241 cgctcttaat tcgcgtaaaa attgttggcc gagttggtct tcttgttgtt tattaacact
     3301 aaatgcccct aaatcgggta ataccgattc gctattggta tcggcaatca gcaaactact
     3361 ggcgaataaa ttccacaaaa taaagctagt ttggcaaaat tgacgtatca taaaatcaaa
     3421 ttctttagaa ataggtctta gcatagggta aataatgaat caacttgatg cacgagggtt
     3481 acgctgtccg atgcccttat taaaattaaa gcaaagttta caccaaatga atgcagggga
     3541 agtgttaaca gtgatgacta ctgaccctat ttcacagcgt gattttgttg tttttttgcg
     3601 acaaagcccg catcagttat taagtatgca ccaagaacaa gacgtttttt attttcaaat
     3661 agaaaaagcg agataaaaca gtctcaactg tgtaaaatac ttagaactta cgtattttgc
     3721 caaaataagc gataactgcg taagtccata attatcttta gttttttagc cattaagcac
     3781 tgtgccatga cacaacatac actccctaca ttaccagaac ttcgtcaaaa aattgatgct
     3841 ttagacgaac aaattcaatt attaatcaat caacgtgctg tttgtgccca acaagtagcc
     3901 gacgttaaaa aagccgaaga cccaaccatt accagctttt atcgtcctga gcgtgaagcc
     3961 caagtattgc aaatggtcat gcagcgtaat caaggcccgt tagcaggcga aactgttgca
     4021 cggttgtttc gtgaaattat gtcggcgtgt ttggccttag aatcaccact caatattgct
     4081 tttttagggc caaacggcac gtttacccat gccgccgcgc tcaagcattt tggtcatgca
     4141 gccattactc aacctatttc aacgattgat gaagtgttcc gtgaagttga agcgggcagt
     4201 gtgaattatg gtgtggtgcc agttgaaaac tcctctgaag gcgtggttaa ccatacttta
     4261 gattgcttta tggcctcgtc tttaaaaatt attggtgaag tagaattacg cattcatcaa
     4321 aacttattag tggcacaagg cacagaaatt aaaaacattt cgcgcattta ttcccaccaa
     4381 caatctttag cgcaatgtcg caaatggtta gatgcacact ttccaaaagt ggagcgggtg
     4441 gcggtgtcaa gtaatgccga agcggcacgg atggtcaaaa atgaatggca ctcagccgcc
     4501 attgcgggtg aaactgccgc tgagctttac ggcttaaata ttttgcatgc taacattgaa
     4561 gataatccta ataacaccac acgcttccta attattggta cagaagaagt gcccgccagt
     4621 ggtgatgata aaacctcatt aatcattgcc gcacataacg aagcgggggc gttgtatcat
     4681 attttagagc cattctatcg tcataatatt tccttaacgc gcattgaaac acgtccagca
     4741 caaaatgata tttggtcgta tgtgtttttt attgattttg aaggtcatca acaacaaccc
     4801 catgtgaaag tggttttgga tgaaattaag gcacgtgtca gggaaatgcg tattttaggt
     4861 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntaaattgt attaccttgg
     4981 gtaatggctc aaatgacatt ttagagttgg ttgccagagc ttatattgct caaggtgaaa
     5041 gtgctgttta tagccaacat gcttttgccg tgtatccttt agcggtgcaa gccgtgggtg
     5101 gggtagggat tgaagtgcca gccaaaaact ttggccatga tttagaggct atggcggctg
     5161 ctattcaagc gaataccaag gttgtgttta ttgccaatcc aaacaaccca acaggcacat
     5221 ggtttgaacg tcacgaattt gaacaattta tggcgcaagt cccagcccat gttttagtcg
     5281 tattagacga agcctatgtc gaatatgtca ctgatgcagg ctttattaat ggctttgatt
     5341 acgttaagca atatgccaat ttaattgtca gtcgtacttt ttctaaagcc tatggtttag
     5401 catcgttacg tgtaggttat gcgattgctt cggctgatgt gaccaatatt ttaaaccgtg
     5461 ttcgccaacc gtttaatgtc aatagttttg ctttagcggc tgcaactgcg gctttgcaag
     5521 atgaaagctt tgttaagcaa agccgtgagt taaatagtgc aggcatggca cagttggaac
     5581 aaggtttaag ctcattggct ttaggcttta ttccgtctaa aggtaatttt attgcctttg
     5641 atacaggtcg tccatgtttg ccagtttttc aagccctgtt acaacaaggg gtgattgtta
     5701 gaccgattgc taactatggg ttgccaaact atttgcgggt cagtattggt ttagaaagtg
     5761 aaaatgcccg ctttttggcg gctttagcac aggtgttacg ctaatgttta accaagtgac
     5821 ctttattggt ttaggactga ttggctcaag tttggctcgc gtcattaggg cgcaaggttt
     5881 ggccaaaaaa attgtcgcca gcagccgtag tgaacgtacc ttacaaacag cgttggatat
     5941 tgctctgatt gatgaagcct ataccgatgc cgctcaagcc gtagcaaatg ccgatgtggt
     6001 cgttttggca atgccagtct gttccactga aaaggtttta gccagcatca aacacgccgt
     6061 tcgtcctaca accattatta ctgatgtggg tagcaccaaa ggcaatgtgg ttgatgcagc
     6121 acgtcgggtg tttggtgatg tacctagcca ttttattcct gcgcatccga ttgctggttc
     6181 agaacaaagt ggagtcttgg caggcaaagt cgatttattt aaaggtcaca aagttattat
     6241 tacgccattg gcgcatagtg acaaacaagc cacacaacaa attcagcaat tgtggcaagc
     6301 tgcaggggct gatgtgttaa cgatgacggt tgagcgtcac gatgaagtgc ttgccaaaac
     6361 cagtcatttg ccacattttt tagccttttc tttggtacat gctttagcca aagaatcgca
     6421 aaatttagat atttttcgtt atgctgcagg tggttttagg gattttactc gtattgcggg
     6481 tagtgaccca accatgtggc acgacatttt tttagccaat aaagccagca ttctcaatgc
     6541 attaacaagc tttaattcag gcgtagagca actgaaagcg gcagtagaag ccgaagatag
     6601 tgaagccatt ttaggcattt taacccgtgc tcaagcagca cgacatcatt ttactcacat
     6661 gttagctgga acagcatacc aaaagactat gaccacgaaa aatttgcaat ttgtccttga
     6721 agcgggcggt actttacaag gtaatttacg tgttgcgggt gataaatcta tttcccatcg
     6781 ttcgattatg ttaggctcga tagccgaagg tgtcaccgaa gtctcaggct ttttagaagg
     6841 tgaagatgct ttagctacct tacaagcttt ccgtgatatg ggcgttgtta ttgaagggcc
     6901 aaaaaatggt gaagtgcgaa ttcatggcgt aggtttacat ggtttgcaag ccccacccaa
     6961 tgccatttat tgtggcaatt ctggcacatc catgcgtttg ttgtcgggta ttttagcggc
     7021 acaaaaattt gatacggtaa tgacaggtga cccttcatta tcaaaacgcc ctatggaacg
     7081 tattgctaaa ccattgcgtc aaatgggtgc agtgattcaa accacaggtg aaaaaggcac
     7141 accgccagtg agcattaaag gcaatcaagc cttacaagcg atgacctacg atatgcctgt
     7201 ggcgagtgcg caagtaaagt caggtatttt attagcggct ttatgggcag agggtgaaac
     7261 ggtcgttacc gagccagcac caacccgtga ccacagcgaa cgtatgttgc gtggttttgg
     7321 ctatgatgtc aaaaccgaag gttcgacgat tcgtattaaa ggtggcggta agttaactgc
     7381 aactaatatt gatgttcctg ctgatatttc ctctgccgca ttttttatgg tgggtgcaag
     7441 tatcgccccg aatgctgatg tggttttaga acacgttggt attaatccaa cacgtattgg
     7501 tgtgattaac atccttaaag caatgggtgg cgatttaacc attatgaacg aacgcgaagt
     7561 cggtggtgaa cctgttgccg atattcgagt gcgtagtgcc aagttaaaag gcattcatat
     7621 tgatgaagct gatgtgccgt tagcgattga tgaatttcca gctttattta ttgccgccgc
     7681 ttgtgccgaa ggtacaacga ttttaacagg tgctgaagag ttgcgggtta aagagtctga
     7741 ccgtattcaa gtgatggctg atggtttgat taccttgggt attgatgcca aacccacacc
     7801 cgatggcatg attattcaag gcggcacaat gggtggtggc aaaattatta gtcatggcga
     7861 ccatcgtatt gccatgtcgt ttgcgattgc ctctttacgc gcaacaggca caattgaaat
     7921 tgaagattgt gctaatgtcg cgaccagttt cccgaatttt gttagcttag caaaacgtgc
     7981 aggcttaaaa attcaggtat tagaagtgta attaatcatt cgatattacc cgtatttatt
     8041 gtgtgaatac gggtaatatg acacatcaaa cagcccaccc agccaaaatc cctaaaccaa
     8101 ccataaatgc tgcaccaaaa ataacggtga gttttttaag tagcacttta tttcccaata
     8161 cagcaacagc tccccattta aaaaccaaat tggccataat agcaatcatg accaattgcc
     8221 aacctgtatg agcttctaaa cgttggcctt caaccaaacg gcctgttgac aaggttattg
     8281 catcaacatc agtcaaaccc gaaatcatgg caattaaata taaactttgt tgtccaaaca
     8341 tttcctttgc ggctgcaacg gcaaagataa ttccaccata cagcaagcca aatacaatcg
     8401 cactagttag ctcagcagga ttatcaggct ctggcatggt atgggtttcg ccactacgac
     8461 taaaatataa agccacacaa ataacaacca taatggcaaa taaggttaat aagggtgggg
     8521 caatgacgct cgttgctgat ggtgaaacga ccataaacgc gagggtgaca cgaattactg
     8581 ataccgtaga ggccaacata attgctaaag ctgctaatga acttgtttcg ggtaaagttt
     8641 tggtgttgcg tgcgtaagaa acggtggtgg ctgtgctaga aattaatccc cctaaaatgc
     8701 cagctaataa cgtgcctgct ttatctttaa ataccttata ggcagcgtaa cccatcagac
     8761 taattgccac aattaatacc accatgcgcc aaatatcgta tggatttaac acatgaaatg
     8821 ggccatagtt ctgattgggt aaaataggca aaatcactaa ggcaatggca gcaaaacgca
     8881 taatagccaa aatatcgcgt tcacccattt tgcgaacaaa gtcgtgtaac acgtctttca
     8941 tttgtagcaa aacagcagtt aatgcaccaa ccacaatggc aattgttgcg tcaccaaacg
     9001 ttagatatgc accaacgata tacattaata aagcagctac ttcggtggta atacccaagt
     9061 cttgattact tttaaaaagt gcaaatttgg cacttaaaat gacaatcgct aaagtaataa
     9121 aaccagaagc taaaatccac ccaccaaaat gttgggcaac taagcctgtc attgcgccca
     9181 ttagactaat taacggaaaa gtccgaatac cagcaagagc agatgcggcc ttttcacgtt
     9241 gtaagccaac caataaacct aaaccaaagg ctgttgctaa aatttgtaaa atgtcattat
     9301 ttatcattag gtaatggtcg ccatacttgg tcaataaggg cttctaaagg gaagaagttt
     9361 tttttgtaag ccattttagg actaccatca acccaatagc ctaagtataa ataatctaag
     9421 ccgatttgtt tggtatgttc gatttgccat aaaatagcat atgttccaat actgcgtttt
     9481 tctaaacgcg catcgtaaaa cgtataaaca gcagataaac cattttttag cgtatcacaa
     9541 gtagcaacag cgactaaacg atcctctagc caaaattcat ataaatgggt atcgctccaa
     9601 tctgaaaaca aaaaagataa atattgagcg accgaaggtg ggtacatatc gccgtcacta
     9661 tgtcgagagc gaatatagtg agcatataag tcataatgat gttgataata acctgcttta
     9721 atacgatgta ctcttaaatc ttggtttttg tgccaaacac gtttttgttg tcgattttgc
     9781 ttgaagtgat gtacgggaat tcgtacagaa atgcacgctt ggcactgtaa gcattgtggc
     9841 ttatagatat agtttccact acgtctaaac ccataacttg ataattggtt ataaagagga
     9901 ttgctcatga ctgcttcggg gtcagcaaat aaagtaattg cttgtttatt atctaaatag
     9961 ctacaggcat gaggaggggt agtaaaaaat tttaaaccag aagcggtcat aattgcgaag
    10021 cccccaagtt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngggtagtaa
    10141 aaaattttaa accagaagcg gtcataattg cgaagccccc aagtttgtaa taagtttttt
    10201 attattttag tcaatttatt attttaaatg aattaaacaa gagtaatttc tttagttgaa
    10261 tgtactacgt tctgaagtgc ttgccaatta ggtttttcaa ggtctttata atctgtcagt
    10321 atgcttaaaa attcagttct aggtatggta taagcaccta agcttaataa atgatcattt
    10381 ggtaattgac aatcaacaat cggaaattgc cacttagcac aaataaacat caaaaaggca
    10441 aaagcaactt ttgaagcatc tgtggctgta ctaaacatag attcgccaaa aaagagtttt
    10501 cctaaattaa taccatataa accacccact aattctcctt gttgattcca tgtttctatg
    10561 gagtgagcaa cacccatttg atgtaatttg ctatagctag caataatttt ttggttaatc
    10621 catgtttcat tcgcataagt tcgtggtgca gcacaacgac gaataacttc tgcaaaacaa
    10681 cgatcaacag taatgctata tttttcactc tttaaagttt ttttcaatga atgtgaggct
    10741 tgataaagtt gaggattaat cacacagcgt gggtctggac tccaccataa tgcaggggtg
    10801 ttacaccaag gtaataatga tttaacatca ctttcgttat accaaggaaa tacgcctgat
    10861 tgataggcaa gcaataaggt ttctggtgtg agatcaccgc cataagccaa tagacccgat
    10921 tcgtcagcat aacgaatgtc aggaaactgt tggctatgtg gaggtaaaaa aactggcata
    10981 attaaaattg ataattaagg cctaaactaa acacgccttc tcgcgtattt ttatcgccat
    11041 catcgtgttt gtaatcgtga ttaatcgtta atgcaatcac tttggtgata ttttgtttaa
    11101 gctgattttg catccgtgaa atagtgttat cttcgcctgc ttcaaccgta cttttatgta
    11161 gaaatttggt gtttggtgta attttccaat cataatcaag acttaacaag gcaattgctt
    11221 catcttttgc gggagcaaaa cgtttttcgc tgtgtctagc accaggccca gcttcaactt
    11281 taaaaaagtg cttttcggtt ttaatgattt cttttcccaa acctaaagat ataaaagcct
    11341 gatactctga gctacttaat aagtcttttt cccattgagt gcgtaaagta aaaaagttac
    11401 gctcaccaaa aaaatgtcgg gctttataac tggctaaata acgctcagta tcattagttg
    11461 ctggtacttc gtcacggaca ctaatgcctt cggcactcac ggtttgtacc caatatgtgc
    11521 cattgtagct tgcagaagtt ttacctctaa aagtttcttt actgccactg gtcgatccca
    11581 tgctactaat ataacctacg tcaatattgc ctttccataa attgttattg tcagcatcat
    11641 cggcatacga ataaaaggaa gtaatactca gcagactagt taagaatttt acaagagcag
    11701 agcgcataaa ttaaatcacc atcaaagaaa agggcagatt gtctgcccca tctatttaag
    11761 cacgacgttt aggtaaaatt tcttttaact tgtcatgcat cttcaatata gcttcttcag
    11821 tggtatccca actgatacaa ccatcagtca ccgacttgcc gtattctaat tgcgataaat
    11881 cagcaggaat atcttggcga ccaaacttca aatggctttc aatcatcaat ccgacaatag
    11941 atttgttgcc ttctaaaatc tgattgctga cattatccat gactaaaggt tgtaactctg
    12001 gttttttatt ggaattagca tgactacaat caaccataat attaggggcg attttagatt
    12061 ttgctaattc ttgctcagct aaagttaccg acactgagtc ataattaggt ttgccatcac
    12121 caccgcgtaa cacaacatga ccgtaagcgt tgcctgtagt gttaataata gccacttggc
    12181 cttgactatt aatgcctaaa aaactatgtg gattttgcac acttaataaa gcattaatgg
    12241 ctacttttaa accgccatct gtaccatttt taaagcctac aggcgaagac aaaccactag
    12301 acatttcacg atgtgtttgt gattctgtgg tacgtgcacc aatggctgac caagaaatta
    12361 aatcgtgcat atattgtggt gacatggggt ctaaggcttc tgtggcacaa ggtaaaccca
    12421 tttcattcaa atcaagtaat aattggcgac caatatgtag gcctttttga atgtcgaaag
    12481 agtcattcat gtctgggtca ttaattaaac ctttccaacc tacggtggtg cgtggttttt
    12541 caaaataaac gcgcatcact aaatataagg tatctttgac tttttctgat aagactttta
    12601 aacgttgtgc gtattctttg gcagcattga tatcatgaat agagcaaggg ccaaccacta
    12661 aaaataaacg atgatctttg ccatctaaaa tattacgaat agtttggcga ccttcaacaa
    12721 cggtttttgc agcgacatca gataaaggaa tatccgcttt taatgctgcg ggagtaatta
    12781 aaatttctgt agagcgtaca ttgacgtctt ctatttgcat ctcaatagtc atattaaatg
    12841 ccatttgtta aaatccgata gagcattcga ttgtagcaaa gatttgttaa aaaacatgaa
    12901 taaataacca caaaaaatag tctttttatt gtttaaaagt aaaaagccac ctaatttgta
    12961 acaaaaaata attaaatatt aagtaataat ctgttacaaa tgctttatct atcatgtaaa
    13021 agtgattgta tatcggaagt tagctgtttt gttgtcaaaa aagactataa taaatagcca
    13081 accttaaaat gttaaaattg gagaaaatta tgcgttcatt ggctgttgca gcgttagctg
    13141 ctagtgttgc agtgacaggt tgtacaacaa atccatatac gggtgacaaa caagcaagca
    13201 aagcagcttg gggtgcagcg attggtgctt tatctggtgc ggctgttggg gcagcaacag
    13261 gtgataacaa aaaagatcgt cgtgagcgtg ctttaaaagg cgcagcgatt ggtggtgttg
    13321 ttggcggtgg ggttggtgca tacatggatg cccaagaaaa gaaactccgt gaaaaattac
    13381 aaagcacagg tgttggtgtt aagaaaaatg ctgatggctc tattgagtta attatgccag
    13441 gcaacattac gttcccaacg ggtcaaagtg ccattaagcc agacttcttt gatactttaa
    13501 acagtgttgc tttagtatta aaagaatata acaaaacatc aattactgtt tctggtcata
    13561 cagacaatgt cggtaaagat gatttaaacc aaaaattgtc tcaagatcgt gctaactctg
    13621 ttgcgcaata tttatctagc caaggtgttg atggtggtcg cattagtgca gtaggtaaag
    13681 gtaaatctga gccaattgct gataatgcca ctgaaaatgg ccgtgcacaa aatcgtcgtg
    13741 ttgaaattac catcaatccg ccagagtctg tgtaacgaat acctaaaaaa taagggctat
    13801 ttagccctta tttttttagt ttggtatata tttcatgcca acttcttttt tggtcatcca
    13861 ccataacaac attaaagagc caccatgtat cgtgctcgta attaaccagt tattattggg
    13921 ggataaatta aaactgacta attgactggc gagtaataca acataaacat gaataataaa
    13981 aacatataaa gaggcttgac ccacaggaat caaataccag cctaatgcct tataaaaagg
    14041 tgtccaacaa atagttaata accaatataa agtgactaat aaacaaaaat catttaatac
    14101 tctaaccact cccaaagagt ttttttgtgc ataattttca taaatataat taaaagtatg
    14161 agcttctatg aggttgagtt ttgcccaact aggtaaaaaa ggattggtgt gattttgagc
    14221 aataaaaaat aaaatcaagc taactgatac acaaaagcta agtagtaata ttttacgcca
    14281 accgtacata tatgaggcaa tttcggcttt aaaccagcca atagccaaag cattaaaaaa
    14341 taaagcttgc catgcgagaa cagcaaaagc attttcaaag ttactattag taatatgtat
    14401 gttaacgtgt ttttgatacc aaaaataaac taaccaacta gccgctaaaa aataccattg
    14461 tttgtgataa cgaaaagcaa caaaagccaa tggcgttatt agcatgagaa tgacatataa
    14521 acctaaaact tgtgtttggt gaggaccagc ttgcagataa aataaaaaat tcaatgcggt
    14581 gtttagtggc acatcattaa cagggtataa aggataaact tgattaatgc ctctattagt
    14641 aaagctaatc acctcatggg cattaataaa tggaaggagt gctaataaat agatgctgat
    14701 gataataaat aaactaacaa catataactt ccaagctcta gaccaaacac gataaatact
    14761 ggtaatccaa ccatcaatcg gtagtcgatg cctatatagg ctgcctaata caatgcccgc
    14821 aaaaatgaca aatccttctg cgccagatac taagccaaaa cgctctgagg taaataaatt
    14881 aaataccgaa aaaacctcaa tgtgtgccac aaccataata atcatagcga taccacgtaa
    14941 aaagtctatg cgtaaatcac gccgaccagc agatgggtaa tgccatccag attcgctaag
    15001 tggaggacgt tctgtatttt tactcattgt atttccttat aaggagagat attctaaaag
    15061 gaattatagg cttatatttt gagagtatta tgcgtacagc cattcatggt cgcaagtttt
    15121 ttctagcaag ttttatcttg cttagtacga atacgcttga ggtaaaagcg agtgaagagg
    15181 atgagcaaaa tcctgtttgg ttggggctaa gcagctcttt tgcctttcca tatagccaat
    15241 tatttataaa accttctgag tcggagtggg ataatttgtt taaaggtagc tcaataagac
    15301 tgtcttatag ccacccatta actaaaacag ttgttgcaca aggcgcaggc aaccaaggag
    15361 aaaccgctac taacagtaca atgcaactcg gtttaaaata tacgccattg tcttattggt
    15421 ttgtgagtgc caattttacc cattacttgc agtcagattt acaaaataca tgggattcag
    15481 attggagtta tagttttggt tatgatgatt ggcatcctta taccttgagt ttgacctaca
    15541 gtaattcggg gggaaatagt ttatccgata aaaagccgcg ttttaatgaa ggaaattggt
    15601 cactaggttg gaagtttact attcccgaaa cagttaaaaa tatttttgtg acaggttatg
    15661 gtgataactt aggctgtggt acaaatatga gtttaacacc acgctatatt gactccttca
    15721 caaatcaaca agcgagtaat aaagttgtgt taagtatgtc ttgcaaatat acaattgttg
    15781 gtgcatggta tatcaacttt tcagtattgc attacccaca aaaagaacag caacaagctt
    15841 ggaatccaga ctatacctat ggttttggtt attttgattg gcggccttct aaaatatctg
    15901 ttcaatataa taattattct ggtaatcgtt ttaatagcgc aaaacgagcc aatggaacag
    15961 gagaatttaa aaatggtagt ttgtcaattg cttggtctaa gtcttggtaa gttaaaggga
    16021 tgattatgga acaagcgtca ataatagcag aacatttatt gccaattctc attgtagatg
    16081 attctaccag ttatcgtttg ttgctcatga gacacttaaa agcttggggt gagtatgaaa
    16141 ttatccaagc tgaagacggt attcaagccc tagagattat taaatcacgt cgtgtaggca
    16201 tggtaattag tgattgggaa atgcctaata tgaatggttt agaattatgt aaagccattc
    16261 gtgaactctc gacaaattat gtttatttta ttttagtgac atcacgagga acttctgaag
    16321 atttaatttt aggtatggaa gcgggtgctg atgacttttt agcgaaacca attaatcagc
    16381 aagaattaaa agtgagggta cgtgcaggtg agcgtatttt acgtttagaa gctcatttag
    16441 aagaaaaaaa tcaacgtttg aatgccactt atcaaattat ggctaacgat ttaaaagcag
    16501 ctgctcaaat gcaacaatct ttattgccgc aagggcaatt aaatatttat aacgttaatt
    16561 ttgattggtt ttttaaacct tcgctgtttg tttctggcga tatgcttaac tattttcgtt
    16621 tagatcatga aaatattggt ttttatagtg ttgatgtatc aggacatggc gtaaagtcag
    16681 caatgttgtc tgtaactttg agtagcttat taaatcataa tcatacaaat agtcttgtca
    16741 aaaaacaaat tcctgatttt ccctattatc gaattagtag ccctaaagaa gttgtaagca
    16801 ctctaaacac gcaatttcag atgactgcgg ataacatgat ctattttacc attgtttatg
    16861 gggtgttaaa cgtttttacg gggcaaggta tgttgacgcg tgcaggtcat actagcccga
    16921 tagtgattca tgctaataat gttgtagaaa ttattgatag tgaaggaggt gtgcctgtag
    16981 gctttttgga agaaccagat tatcaagaag ttgcttttag tttagaaaat gactctcgtt
    17041 tatatttata tacagatggg attactgaat gtgaaaataa attgtcagaa caatttggtg
    17101 aacagagatt gatagattta ttagacaaga atcatgagtt accactacaa caaacacttg
    17161 ctattttaca acaggagtta aatctttggc gaggaggtga gcaagaggcc tttacggatg
    17221 atgtttctat gctaacgatt cattatgttg gtaatagtct caattaactc aatagacctt
    17281 aaacacaata aaaaacggcc aaataaatgg ccgtttttta ttgttctaaa ttcaagcaac
    17341 ttgcacagga atagcattgg cactatggct aacagtattg tctgggttgc aataaa
//
LOCUS       DAOQMB010000009        17081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1198_length_17081_cov_16.801128, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000009 DAOQMB010000000
VERSION     DAOQMB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1198_length_17081_cov_16.801128"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLY05_00855"
     CDS             <1..261
                     /locus_tag="PLY05_00855"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNC01880.1"
                     /translation="KKAADKDKSEAWVVQVASVSDKAKADALSSKLAGKGYRSRVVKN
                     GDTWKVVVGPELDKGKAQSLKSKLSGESALGVTGAWVAPWKP"
     gene            249..773
                     /locus_tag="PLY05_00860"
     CDS             249..773
                     /locus_tag="PLY05_00860"
                     /inference="COORDINATES: protein motif:HMM:NF022337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HNC01881.1"
                     /translation="METITTVLVIVLCVIGLLMLVALLARKPNKPLANKEPKQSVDEM
                     KVWPYVARELMTAPERELYGRLRQALPDYLIFSQVQLSRIIDVAPEAEHQAWLNRINR
                     MSVDFVICAADGAKILAAIELDDSSHDRPERIKADSKKDKALLSAGVAIIRWPVKGMP
                     NPRQIRADFFKYTR"
     gene            786..>1885
                     /locus_tag="PLY05_00865"
     CDS             786..>1885
                     /locus_tag="PLY05_00865"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNC01882.1"
                     /translation="MSITTWVVACADGLENLLAEECQEIGATVLRQEPSAVWVEGDLR
                     TAYNLCLWSRLASRVYKPLFRIHSNTPEDLYQEAVRYPWHEVFSLDKTFAVRAVAIKG
                     VVCHTQYMALKLKDAIVDAFRRQTGERPSVDPQASDINLHVLVKPDSITVSLDMSGES
                     LHKRGYRRMIGEAPLKETLAAAILRQAGWPNNRFQALIDPMCGSGTLLTEAALMYGDV
                     ATGLMRDYFGFLAWEGHNQALWEQCWQEARQRQQAGFQKVWPVMQGYDADMEALQAAS
                     KNIQAAGLSQFVQLKHQALVDLPIKPTNDGLLITNPPYGERLGEDDTAIFLYKALGRL
                     ARERLGGWQATVLATKIEHADALGFEHLHTQKL"
     assembly_gap    1886..1985
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1986..2921
                     /gene="rlmKL"
                     /locus_tag="PLY05_00870"
     CDS             <1986..2921
                     /gene="rlmKL"
                     /locus_tag="PLY05_00870"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HNC01883.1"
                     /translation="VEADAFANRLRKNLQHFYKQAVREQVCCYRVYDADLPEFNLAID
                     IYGEVLHVQEYAPPKSIDTEKAAARFKMALLVIRQIFGLHRDKVFIKVRAQQKGQQQY
                     EKLNQRNKYLEVQEGKARLLVNLTDYLDTGLFLDHRPIRQKISQQSADKSFLNLFAYT
                     CSASVHAALGGANKTVSVDLSNTYLLWGKKNFALNGLDERYHQFIEADVLSWLRQCRD
                     KFDMIFIDPPTFSNSKKTQDVFDVQRDHVELLTLAMRLLKPNGICYFSTNFRRFELDS
                     EAMEYLHCQEISHETLGFDFKRNHKIHRCWQVSHA"
     assembly_gap    3081..3180
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3576..3959
                     /gene="pilG"
                     /locus_tag="PLY05_00875"
     CDS             3576..3959
                     /gene="pilG"
                     /locus_tag="PLY05_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilG"
                     /protein_id="HNC01884.1"
                     /translation="MEENFDGLKVMVIDDSKTIRRTAETLLQKAGCTVITAIDGFDAL
                     AKIADSNPDIIFVDIMMPRLDGYQTCALIKNNSAFKSTPVIMLSSKDGLFDKAKGRIV
                     GSDEYLTKPFSKEELFTAIRQYVKS"
     gene            3989..4357
                     /gene="pilH"
                     /locus_tag="PLY05_00880"
     CDS             3989..4357
                     /gene="pilH"
                     /locus_tag="PLY05_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004640626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility response regulator PilH"
                     /protein_id="HNC01885.1"
                     /translation="MARILIVDDSPTETYRFKEILERHGHKVLEASNGADGVAVARAE
                     LPDLVLMDVIMPGINGFQATRQITKGAETAHIPVVIVTTKDQETDRVWGKRQGASDYL
                     TKPVDETLLMNVIRTLIPNK"
     gene            4366..4896
                     /locus_tag="PLY05_00885"
     CDS             4366..4896
                     /locus_tag="PLY05_00885"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC01886.1"
                     /translation="MAAKGFIKLLEIAGRSKQKNLGLLGQGEQSWTGVGFVLAGEYFL
                     APIGEVAEILKLPRYTLIPGVELWMKGLANVRGRLLPITDILMFLGRKSTVLDNKRRV
                     LVVDHEEMFAGLIVDEVLGMQHFNIQDFSLAVKAPFGEVSPFVRGAFVSEGKTWYVLL
                     LSRLVEDPRFLKAAVA"
     gene            5107..7221
                     /locus_tag="PLY05_00890"
     CDS             5107..7221
                     /locus_tag="PLY05_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNC01887.1"
                     /translation="MKSPNLRKPFSGISAKKLLSKQTFQTLFSRGFWRSLADTPDATR
                     FLALFLISILVTAGAFIVVAKKSQNGRVMIQHASELRVLSQDIAKNATEASSGNQQAF
                     DFLNRAVGDFSKRWTTLKDMHGETRADTKAVQAVWQRVSDNLQKVSAGEKTVGNLHDL
                     VAGIADAVPELQAENDSIVDAMVASGAPASQVVIAKNQSVFAERILRSVSSVLNGDEN
                     AVMAADDFGRDSEAFGQTLEALLNGDPDMGITAVKDPQARASLEAIKKLFESSVQQGA
                     NEILQSSPELFQVREASAAIFRDSPELLSTLTKLTGVVDEEANAIVPSIIGVIFLVST
                     LGSLFGFIRIRARQDKERAEKEAEFDRKRAEEVEMENQRNQSAILRLLDELGDLADGD
                     LTVQATVSEDFTGAIADSINYSIDQLRQLVSTINQTAVQVSAAAQETQSTAMHLAEAS
                     EHQAQEIAGASAAVNEMAVSIDQVSANAAESAVVANRSVAIAHKGADVVQRSIEGMDT
                     IREQIQETSKRIKRLGESSQEIGDIVSLINDIADQTNILALNAAIQASMAGEAGRGFA
                     VVADEVQRLAERSASATKQIEQLVKTIQSDTNEAVISMEQTTAEVVRGARLAQDAGVA
                     LEEIQTVSRNLADLIQNISNAARQQAASAGHISNTMNVIQEITTQTTAGTIATARSIG
                     NLAEMAAELRLSVSGFKLPDYA"
     gene            7244..8107
                     /locus_tag="PLY05_00895"
     CDS             7244..8107
                     /locus_tag="PLY05_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNC01888.1"
                     /translation="MGLQDWGIRPVTPISDEQFALWQGLIESKTGMIFTQTRRPFLEI
                     SLSARMREIGAEDYDTYYELIASGARGEREWLTLVDRLAVQETSFFRHASSYELVESY
                     IKNVLALHQKKSLNLWSAGCSTGEEPYSLALLVEALLASSGRRDIFYGITATDISLPT
                     LAKAKLGIYSERKLFGVPETLRQTYFRPLDNQRDWQIDQSLRARVAFAQLNLMELDKA
                     PFHEMDIIFCQNVLIYFRRFRKRDVVTHLAERLQVGGMLILGVGEVLDWHHPTLERVD
                     YPNTLAYRRVV"
     gene            8171..>11986
                     /locus_tag="PLY05_00900"
     CDS             8171..>11986
                     /locus_tag="PLY05_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC01889.1"
                     /translation="MTDNHNFLALDWVKSEIQETLKQAQQALEAYIATPNDTAKLRFC
                     LAYLHQVFGTLQMVEFYGAAMLAEEMERLCLSLVNHKVSDRAKAQHVLIEAMLQLPAY
                     LERLQVAKKDTPFVLLPIINELRTAHDETPLSELALFKPDFARVFSVIGQTGVAPIQD
                     SLKKLRHGYQLSLLGIIRQQNLLQQYEHLILVFEKLGLFVAQKPLQALWQTSSALVEG
                     LMSNDIPLTPAIKTLLGQIEQELRSYIAYPQHSPSLDLLKNILYYIAKSRGTTHRMLA
                     VRALYHLDEILPTFDVVEAEKEKLAAPDRQAMQSVIKGVTDELSHVKEVIDDLTYATH
                     PDIDGLRPLLPILRQIASTLQVLGLYQLQRIISEQIRALNKSLNFGVLPEGALLVVAS
                     GLIEVESSLQQLVEDQPLTPNLENFGGNAMVDQVSAAQGAVLRESRTALEKAKELIVE
                     YISSQWNAEKLAELPVLLNTVRGGLSMLDLSRPAQILARLSEFVTKHLLKDECRPSWS
                     EMDTLADIISSVEYYLERLANHHQNNNLLDVAAQSLEKLGYGLLLTTPTTSDSVERRA
                     VAKTEEEKALPAIDDIDFGEPPPSMLDKTIVNMPALVLDDNAVSDKPVQQEDNVDKTL
                     IAMPALTDEMLARAAQTVEDDGEKTLVNLPAVSLPSKSEPSSEDMFSSTMTFNAEDYL
                     SEGVFAPKSQAVVIEEPASPISFDDTESMFKPEGVELAPTMVNMPVVNLDETQRLPIV
                     EDISITEANDEIDPDATVKIVMAETQSVEEVPLETELYEQQTIAVAFEITAQIPEDDF
                     SEDDEIREIFIEEAEEVLETLNSYLPKWSANFDNKAALTEVRRAFHTLKGSGRMVGAK
                     VVGELAWSIENMLNRVIDKTTQPTAAMMQLIAGVVELVPTLMTNFQDKQVPSYKTDGL
                     MTAADLFAKAQSLSSDDVQWAIDYTYNRLVTSTSVADVIMQEEPEIELAIEPVVVADV
                     EEDTDLGNVTIPVMAAINVPEPKDDLETQEDQSAEAISFEDFDDEGVFDVPMDMGEET
                     DLTAAPELLPQQEAVVISEVPNEVISIAKPALTIDIPQHIPVPEDDFSQDDEIREIFI
                     EEAQEVLETINEYFPKWAAKFDNKTALTEFRRGFHTLKGSGRMVGAKVEGELAWSIEN
                     MLNRVIDNTIKPNAELVALISHVIEVIPQLITNFQNKEAPSFNSSPIMSVADNLARGK
                     QVTMEELNAAIAFAHGQVPATVEPVEEVALVQEIAVSDSVATEDSQDYSNAFEDLAKT
                     LSEIKDETTA"
     assembly_gap    11987..12086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12087..15564
                     /locus_tag="PLY05_00905"
     CDS             <12087..15564
                     /locus_tag="PLY05_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC01890.1"
                     /translation="DTDKFIAHVAEVSQAPAADDDEVIESAAPINTAGIVAGFLDLGL
                     ESLMDAPWELAEWLASEQKVEHIQNLVKDLELLAPAAKAAIVMPLVDIVNQLTVVYQF
                     VLEDIEKALSNGQLLEDLANAHDEIINIFDTLAACQTVQPNPKLLERLREWGGSPVDF
                     VDTAAIPSYVPASQEIDEGADPELLAIFLEEAEEVLTAADDELIAWKANPEDKNPLRV
                     LQRHLHTLKGGARMAVVTSLGDLAHELEFLYEDLVNGRYQGTTVLMDLLQRCHDRLAD
                     MIDGLKAEGRCYYAKDLVQVIDIYRRQPTDNVILDFFSSAAGGSTKPVEVPVATSAAQ
                     PTPVAEQPVNVVQPTPQAAVVSNRKAETDEELDPEMLSIFLEEAQEIVDETNASLQQW
                     MQQPEDVTPVQILQRGLHTLKGGARMSGVNSLGDLAHEMENIYEGVCQKRYQGNKEVF
                     SVLQRCHDRIAEVVDNLKDQGRALVVTDLVEQLKRYLSNPQAFVDTVQMAVATPTAVT
                     PVTTDNDVVPVAVVVEAIDDNVMPKMLGNFRDAAKNQQAQEMIRVSSELMEKLINIAG
                     ETSIVRSRVEMGVHSFSQTVEEMGATVQRLAEQLRRMQGELESQILAQHTTEDTKYAD
                     FDPLEMDQYSSLNQLSKSLYESASDLMDLKGTLIDKTRDTETLLLQQSRLNTEMQEGL
                     MSSRLVPFSRLVPRLQRIVRQTATELHKPAELYVVNAEGEMDRTLLERIVAPLEHMLR
                     NAVDHGLETPPDRAAAGKEAMGRITLSLSREGGEIVLTLADDGRGVNVEAVRKKAIER
                     GLLQADATISEKELLQFLFHAGFSTAQKVTQISGRGVGMDVVQSEIKQLGGTVVIQSV
                     AGKGTTFVMRLPLTVAVSRALMVRIGEDVYAIPLSQIEGIVRANPYELETYYAPNAPA
                     FEYANIGYKLHYLGEFVHGIRVPSLFGQTLPLPILLVRGADQRVAIQVDQLIGSREVV
                     VKSVGAQLASVAGISGATILGDGSVVIILDTIAMLRAAALQKPRQRVAEDDTPAIIKP
                     ERATKMVMVVDDSVTVRKVTSRLLERHGYEVVLAKDGLDAITKLEDVRPDIMLLDIEM
                     PRMDGFEVASLVRHNPNLIGLPIIMITSRTGEKHRERAFQIGVNAYMGKPFQEQQLLE
                     TISELLVAASTK"
     gene            15574..16590
                     /locus_tag="PLY05_00910"
     CDS             15574..16590
                     /locus_tag="PLY05_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNC01891.1"
                     /translation="MAKRNPKVAIVSDISLQRLALAHAVKGQGYDLVLNSSPERLDDR
                     LFKSVEPDVWIVDLQNEDDELLDRILDLGVPVLFGLDAAPAQGTRQYPRWERRVFIKL
                     YDVVGHPVIQENLEPLEKQAVAPVAPREIYVPQELMTYKSQRLEFVCVLAASLGGPAA
                     VKEFLDFIPSGLPVAFVLAQHIDARMQESLTRVLVRHNHMPCHIAHDGDKLKSGQLLI
                     APVETEIDFSADGQVISRNIKWDGPYSPSIDQVLANVGRRFGKKSIAIIFSGMGSDGS
                     ISGPQMVEAGGSVWAQDEKTCACPSQPDAMRATGCVSFSGTPFDLANRLVMHISRNLS
                     AHTA"
     gene            16605..>17081
                     /locus_tag="PLY05_00915"
     CDS             16605..>17081
                     /locus_tag="PLY05_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNC01892.1"
                     /translation="MATQAITLSQTTLLSATTGKLDVYILPIWQKKLLLPTVAVVDIL
                     PASQLKTTKKNKKVGILGQIVWEQETIPVIAFETFNGESQTAEPQKIAIMVAATLGEL
                     QYYGLALQAEPTTAKVKIAEIEDLEQAAIGPMEYLQIRYQNQLCVIPDLDAVEQNLA"
BASE COUNT         5052 a         2869 c         3847 g         5013 t
ORIGIN      
        1 aaaaaagcgg cggataaaga caaaagtgaa gcatgggtag tacaagtagc gagtgtcagc
       61 gataaagcta aggccgatgc tttaagtagt aaactagcag gaaaaggtta tcgtagtcgt
      121 gtggttaaaa atggtgatac atggaaagta gtagttgggc cagagcttga taaaggcaaa
      181 gcacaatcat taaaaagtaa attaagtggg gagtctgctt taggcgtaac aggcgcgtgg
      241 gttgcaccgt ggaaaccata accactgttt tagtcattgt tttatgtgtg attggtttgt
      301 tgatgttggt ggctttatta gcacgcaaac ctaacaagcc ccttgcgaac aaagagccta
      361 aacaatcagt tgatgagatg aaggtgtggc cgtatgttgc tcgagagtta atgactgcac
      421 ctgaacgaga gttgtatgga cgattacgtc aggcattgcc tgattatttg atctttagcc
      481 aagtgcaact cagtcggatt attgatgttg cccccgaagc cgaacatcaa gcatggttaa
      541 atcgtattaa tcgtatgagc gtcgattttg ttatttgtgc agcagatggt gctaaaatat
      601 tggcggctat tgagttagac gatagtagtc atgatcggcc agagcgtatt aaagcagata
      661 gcaaaaaaga caaagccttg ttatcggcag gggtagcgat tattcgttgg ccagtcaaag
      721 ggatgcctaa tcctcggcag attcgtgccg attttttcaa atatacacgt taagaaagtt
      781 aaattatgtc tataaccaca tgggttgttg cttgtgccga tggccttgag aacttattag
      841 ctgaagaatg ccaagagatt ggcgcaacag ttttgcgtca agagccaagt gctgtttggg
      901 tcgaaggtga tttacgcacc gcctataatt tatgtttatg gtcacgttta gcctctcgtg
      961 tatataagcc tttgtttcgc atccacagta atacgcccga agacttatac caagaagcag
     1021 tacgttatcc gtggcatgag gtatttagcc ttgataaaac ttttgcggta cgtgcggtgg
     1081 caattaaagg tgttgtctgt catacccaat atatggcact caaattaaaa gatgccattg
     1141 ttgatgcttt tcgtcgccaa acaggtgaac gtccaagcgt tgatccgcaa gccagtgata
     1201 tcaatttaca cgttttagtt aagccagaca gtattactgt tagtttagat atgtcaggtg
     1261 aaagtctaca taaacgtggt tatcggcgca tgattggtga agcaccactt aaagaaacgc
     1321 tggcagccgc cattttgcgt caagctggct ggccaaataa tcgttttcag gcattaattg
     1381 atcccatgtg tggatcaggc acgttattaa ccgaagccgc tttaatgtat ggtgatgtgg
     1441 caacgggtct aatgcgcgat tattttggct ttttggcatg ggaagggcat aaccaagcac
     1501 tttgggaaca atgttggcaa gaagcacgtc aacgccaaca agcaggcttt cagaaagtgt
     1561 ggccagtcat gcagggttat gatgccgata tggaagcttt acaagctgct agtaaaaata
     1621 ttcaagccgc tggtttaagc caatttgtgc aattaaagca tcaagcatta gtcgatttac
     1681 caattaagcc aacaaatgat ggtttattaa ttactaatcc accttatggt gagcgtttag
     1741 gcgaagacga tacggcaatt tttttatata aagccttagg acgtttagcg cgtgaacgtt
     1801 tgggtggttg gcaggcaaca gtcttggcta ccaaaattga acatgctgat gccttgggtt
     1861 ttgaacattt acatacccaa aaactnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnngttga agcagatgct tttgctaatc gtttacgcaa aaatttacag cacttttata
     2041 aacaagcggt gcgtgagcaa gtctgttgct atcgtgttta tgatgcagat ttgccagaat
     2101 ttaatttggc gattgatatt tatggggaag tcttacacgt acaagaatac gccccaccca
     2161 aatcaattga taccgaaaaa gcagccgctc gttttaaaat ggcattgcta gtgattagac
     2221 aaatctttgg tttgcatcgt gataaagtgt ttattaaagt gcgtgctcaa caaaaaggcc
     2281 agcaacaata cgagaagctc aatcagcgta ataaatacct tgaggttcaa gagggcaaag
     2341 cgcgtttatt agttaactta accgactatt tagatacagg cttatttttg gatcatcgcc
     2401 ccattcgcca aaaaattagt caacaaagtg cagataaaag ctttttaaac ttgtttgctt
     2461 atacttgctc tgccagcgtc catgcggcat tgggaggagc aaacaaaacc gtcagtgtcg
     2521 atttatccaa tacctattta ttatggggta aaaagaactt tgcattgaat ggtttagatg
     2581 aacgttatca tcaatttatt gaagcagatg tgttgtcttg gttaagacaa tgtcgtgata
     2641 aattcgacat gatttttatt gatccgccaa ccttctcaaa ctcaaaaaag acacaagatg
     2701 tatttgatgt tcaacgtgac catgtggaac tcttaacctt agcaatgcgc ttattaaaac
     2761 cgaatggcat ttgctatttc tcaactaact ttaggcgttt tgagttagat agtgaggcga
     2821 tggagtattt gcattgccaa gaaattagcc atgaaacatt aggttttgat tttaagcgta
     2881 atcataaaat tcatcgttgt tggcaggtga gtcatgcttg agcaacaaca atggttgcta
     2941 tatgggacaa aaggctgtca tttatgtgat aatgctgaaa gtttattaat gcaggctcaa
     3001 tgtgtattgc caatgacatg gcaatatatt gatattgcat tggatgaaaa tttagtgaca
     3061 caatatggca gcaaaattcc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 aaaatgtgac aaaatcgaca ctacaagaac aaagtttaag gttattatgc taaacctttc
     3241 atcatgataa ctttgtataa agcatgaaaa agtcatgcta agcctttgta atatatttaa
     3301 gcatagtgtt tggtaaagat gattcaacta tggattcatg ctaaaaacta tgttaaaaca
     3361 gggtaactct aggcactcaa agcattttaa acattgtgac ttaggcactt cattaaaatt
     3421 agcgtgtttt atgaatataa aactgttaaa actagtagaa atgttcataa atatgcgata
     3481 actgcgtaag ttctaaaatg aatagcgcac tagaggtata attttaaatc caccaatggc
     3541 ctcagtggcc taataattat aggaaggcaa ttccgatgga agaaaatttt gatggcttaa
     3601 aagtcatggt tattgatgac tctaaaacaa tccgtcgtac tgcagagaca ttattgcaaa
     3661 aagcaggttg tactgtaatt acggcgattg atggttttga tgctttagca aaaattgcag
     3721 actctaaccc tgatattatt tttgttgata ttatgatgcc gcgtttagat ggttatcaga
     3781 cttgtgcatt aatcaaaaat aattctgcat ttaaatctac accagtgatc atgttgtcaa
     3841 gtaaagatgg tttatttgat aaagccaaag gccgtattgt tggttctgat gagtatttga
     3901 ctaagccgtt tagtaaagaa gagttattta ctgctattcg tcagtatgtc aagtcctaat
     3961 ctgtcaatag aaaaataatg aggacagcat ggctcgtatt ttaattgtag atgattcgcc
     4021 aactgaaacc tatcgcttca aagagatttt ggagcgtcat gggcataaag ttttagaagc
     4081 tagtaatggg gcagatggtg tagctgtggc tcgtgcagag ttgccagacc tcgttttaat
     4141 ggatgttata atgcctggta ttaatggttt tcaggcgaca cgccaaatta ccaaaggtgc
     4201 agaaacggct catattcctg ttgtgattgt cacaaccaaa gatcaggaaa ctgatcgtgt
     4261 ttggggtaaa agacaaggtg ctagcgacta tctgactaag ccagtagatg aaacattatt
     4321 aatgaatgtt attcgtacct tgattccaaa taaataggag ggggtatggc agccaaaggc
     4381 tttattaaac tattagaaat agctgggcga tccaaacaaa aaaatttggg cctattaggg
     4441 cagggagaac aaagctggac tggtgttggc tttgtattag caggtgagta ttttttggct
     4501 cctattggtg aggttgctga aattttaaaa ctgccgcgtt ataccttaat tccaggtgta
     4561 gagctgtgga tgaaaggatt agctaatgtt cgtggtcgtt tattgccaat caccgatata
     4621 ttaatgtttt tgggacgcaa atctaccgtt ctagataata agcgtagggt attggttgtt
     4681 gatcatgagg aaatgtttgc aggactcatt gtggatgaag tattgggtat gcagcatttt
     4741 aatattcagg atttttcttt ggcagttaaa gctccttttg gtgaggtatc tccttttgtg
     4801 agaggagcat ttgtttcaga aggaaaaact tggtatgtct tattgttaag tcgtttggtt
     4861 gaagatccgc gtttcttaaa agcagcagta gcttaaaaac aaaaactgtt gtgtcgaggc
     4921 gtgataattg gattgcttat aaacaaaaga gtttgctatg tcagtttagg tgatgacagc
     4981 ctacacaaca catagctcta tatttagttg gattaattgg actgagagtt tttgatagtc
     5041 tttatttgaa actccgttct tattattaaa gaaaataaaa acaggttgtg tggagatgta
     5101 tccgctatga agtcacccaa tttgcgaaag cctttttctg gtataagtgc caaaaaactg
     5161 ctatctaaac aaacatttca aactttattc agtcgtggct tttggcgttc tcttgcagat
     5221 actccagatg caacacgctt cttggcgttg ttcttgattt ctattttagt cactgctggt
     5281 gcctttattg ttgttgctaa aaagagtcaa aatggtcgtg tgatgattca gcatgcgtca
     5341 gaattgcgtg tgctatctca ggatattgct aaaaacgcga cagaagcttc ttcaggcaac
     5401 caacaagctt ttgacttttt gaatagagcg gtgggggatt tctctaaacg ttggacaaca
     5461 ctcaaagata tgcatggcga aacacgtgct gataccaaag cagtacaagc ggtgtggcaa
     5521 cgtgtgtctg ataacttaca aaaagtgagt gcgggtgaga aaactgtagg taacttgcat
     5581 gacttggttg ctggtattgc ggatgcggtg ccagagttac aagcagaaaa tgatagtatt
     5641 gttgacgcga tggtagcttc aggcgcacca gcttcacagg tggtgattgc gaaaaaccaa
     5701 tcggtatttg cggaacgtat tttacgttcg gtaagtagtg tgctgaatgg tgatgaaaat
     5761 gcggttatgg ctgctgatga tttcggtcgt gactctgaag catttggtca aactttagaa
     5821 gcgttattga atggtgatcc agatatgggg atcactgcag ttaaagatcc tcaagctcgt
     5881 gcctccttag aagcgatcaa aaagctattt gaaagctctg ttcagcaagg tgctaatgag
     5941 attttacagt cttcaccaga attattccaa gtacgcgaag cttcggcagc gattttccgt
     6001 gactctccag aattattatc taccttaact aaattaacag gtgtcgttga cgaagaggct
     6061 aatgcgattg ttcctagtat cattggtgtg attttcttag tatctacttt aggctcctta
     6121 tttggtttta tccgtattcg tgcgcgtcaa gataaagaac gtgcagaaaa agaagctgag
     6181 tttgaccgta aacgcgctga agaagtagaa atggaaaacc aacgtaacca atctgcgatt
     6241 ttgcgtttat tggatgagtt gggtgatttg gcggatggtg atttaaccgt acaagcaacg
     6301 gtatccgaag actttaccgg tgcgattgct gactctatta actactctat tgaccaatta
     6361 cgtcagttgg tatcaacgat taaccaaaca gcagtgcaag tttctgctgc ggctcaagaa
     6421 actcaatcga ctgcgatgca cttagctgaa gcttctgagc atcaagccca agaaattgca
     6481 ggtgcttctg ctgcggtaaa tgaaatggcg gtttctattg accaagtatc tgccaacgct
     6541 gctgaatcag cagtcgttgc gaaccgttct gtagccatcg cccacaaagg ggcggatgtg
     6601 gtgcaacgtt ctattgaagg tatggatacg attcgtgagc aaattcaaga aacttctaaa
     6661 cgtattaaac gtttgggtga atcttctcaa gagatcggtg atatcgtgtc tctgattaat
     6721 gatattgctg accaaacaaa cattctcgcg ttaaatgctg cgattcaagc gtcgatggcg
     6781 ggcgaagcag gccgaggctt tgcggtcgtt gcggacgaag tacaacgtct tgcggaacgt
     6841 tccgcttctg caactaaaca gattgaacag ttggttaaaa cgattcaatc tgatactaac
     6901 gaagcggtta tttccatgga acaaacaact gctgaggtag tacgtggtgc tcgtttggct
     6961 caagacgcgg gtgtggcact tgaagaaatt caaacggtat cgagaaactt agcggacttg
     7021 attcaaaaca tttcgaatgc tgctcgtcag caagcggcat ctgcaggtca catttctaat
     7081 acgatgaacg ttattcaaga aattaccact caaacaactg ctggtactat tgctactgct
     7141 cgttcgattg gtaacttagc agaaatggct gctgaactac gtttatcagt atctggcttc
     7201 aagttaccag attatgctta actttaatta ggagtgaact ggcatgggtt tacaggattg
     7261 gggaattcgg ccagttactc ctatttcaga tgagcaattt gcattatggc agggattgat
     7321 tgagtctaag acgggaatga tttttactca aacccgtcgc ccatttttag aaatatcgtt
     7381 atcagcacgt atgcgtgaaa ttggcgcaga agattatgac acttattatg agttgattgc
     7441 ttctggggct agaggcgagc gagaatggtt aaccctcgtt gaccgtttag cggtacagga
     7501 aacctctttt tttcgtcatg ccagttctta tgagctggtt gagtcgtata taaaaaatgt
     7561 attggctctt catcaaaaaa agagcctaaa tttatggagt gctggttgtt ctacaggtga
     7621 agaaccttat tcattagcat tgttggtaga agctttatta gcatcatcag gtcgtagaga
     7681 tattttttat ggtatcactg caactgatat tagtttgcca acattagcta aggcgaaatt
     7741 aggtatatat agtgagcgta agctttttgg tgtgccagaa accttacgtc aaacatattt
     7801 tcgcccttta gataatcaac gagattggca aattgatcag tccttaagag ctagagttgc
     7861 ttttgctcaa ttgaatttaa tggaattaga taaagcacct tttcatgaaa tggatattat
     7921 tttttgtcaa aatgtgctga tttactttcg tcgttttcgt aaacgagatg ttgtaacgca
     7981 cttggcagaa cgtttgcaag tgggtggtat gttaattttg ggtgtaggag aagtgcttga
     8041 ttggcatcat ccaaccctag agcgtgtgga ttatcccaat accttagcat atcgccgtgt
     8101 agtataaaaa attcgaattt gcaattgatg ctgtggtaaa aagcatcatc cccagagggg
     8161 gggtaataag atgactgata atcataattt tcttgcttta gattgggtta aaagtgaaat
     8221 acaagaaacc ttaaagcaag ctcaacaagc tttggaagcc tatatagcga cgccaaatga
     8281 tacggcaaaa ttgcgcttct gcttggctta tttgcatcaa gtatttggta ctttgcaaat
     8341 ggtcgagttt tatggtgcag ccatgcttgc agaggaaatg gaaagattat gtttgtcgtt
     8401 agtaaatcat aaagtcagtg atagagctaa agcgcagcat gtactcattg aggcaatgtt
     8461 acaattgcct gcctatttag agcgtttgca agttgcaaaa aaagatacac cttttgtctt
     8521 gttgccaatt attaatgaat tgcgtacagc acatgatgaa acgcctttgt cagagttggc
     8581 attgtttaaa ccagattttg cacgtgtatt ttcggtcatt gggcaaacag gtgtagcacc
     8641 aattcaagac tctttaaaaa aattaagaca tggctatcag ttaagtttgt taggaattat
     8701 tcgtcagcaa aatttattac aacaatatga gcatttgatt ttagtttttg aaaaacttgg
     8761 tttgtttgtt gcacaaaaac cattacaagc attatggcaa acttcttctg ctttagttga
     8821 aggcttgatg agtaatgata ttccattaac gcctgcaatt aaaactttat taggacagat
     8881 tgaacaagaa cttagaagtt atatcgctta tcctcaacat agtccatcct tagatttatt
     8941 aaagaacatt ttatattata tagctaagag tcgaggcaca acacatcgga tgttggcggt
     9001 acgcgcttta tatcatttag atgaaatttt accaactttt gatgtggttg aagcagaaaa
     9061 agaaaaacta gccgcaccag atcgtcaagc aatgcaatcg gtgattaaag gtgtcaccga
     9121 tgaattaagt catgtgaaag aagtgattga tgacttaacg tatgccacac atccagatat
     9181 tgatggctta cgtcctttgt tacctatttt gcgtcaaatt gctagtactt tacaggtatt
     9241 aggcttgtat cagttacagc gtattatttc tgaacaaatt agagcactaa ataaatcgct
     9301 taactttggc gttttgccag aaggtgcgtt gttagttgtg gctagtggtt taatagaagt
     9361 agagtctagt ttacagcaat tagtagaaga tcagccttta acaccaaatt tagaaaattt
     9421 tggtggtaat gcaatggttg accaagtgtc tgctgctcaa ggggctgtgc ttagagaatc
     9481 tagaactgct ttagaaaaag ctaaagagtt gattgttgaa tatattagct ctcaatggaa
     9541 tgcagaaaaa ctagccgaat tgcctgtatt attaaataca gtgcgcggtg gtttatcgat
     9601 gttagattta tctcgccctg cacaaatttt agcgcgttta tcggagttcg tgaccaagca
     9661 cttattaaaa gatgaatgtc gtccaagttg gtcagaaatg gatactttgg ctgatattat
     9721 ttcatctgtt gagtattatt tggaacgttt agcaaatcat caccaaaata ataatttgtt
     9781 ggatgttgct gctcaatcct tagaaaaatt aggttatggt ttgttactaa caacacctac
     9841 tacatctgac tctgtggaga gacgtgcagt tgcaaaaact gaagaagaaa aagcattgcc
     9901 cgcaattgat gatattgatt ttggtgaacc accaccatct atgttggata agacaatagt
     9961 aaatatgcct gctttggtct tagatgataa tgcagtaagc gataagcctg ttcagcaaga
    10021 agacaatgta gataaaacat taattgcaat gccagcatta acagatgaaa tgttggcaag
    10081 agcagctcaa actgtagaag atgatggcga aaaaacattg gtgaatttac ccgcagtaag
    10141 tttgccgtca aaatcagagc catcatctga ggatatgttt agtagtacca tgaccttcaa
    10201 tgcagaagat tatctctctg aaggagtatt tgcacctaag tcacaggcag tcgtgataga
    10261 agagccggcc tcgcctatta gctttgatga tactgaatct atgtttaagc ctgaaggtgt
    10321 tgaactagca ccaacaatgg tcaatatgcc tgttgttaat ttagatgaga cccagcgttt
    10381 acctatcgtt gaggatattt caataacaga agcaaatgat gaaattgatc cagatgctac
    10441 tgtaaaaata gtgatggcag aaacacaatc tgttgaagag gttccattag aaacagaatt
    10501 atatgaacaa caaacaatag ctgtcgcttt tgaaattact gcacaaatcc cagaagatga
    10561 ttttagtgaa gatgatgaaa ttcgcgaaat tttcatagaa gaagcggaag aagttttaga
    10621 aacgttgaac agttatttgc ctaaatggtc agcgaatttt gacaataaag ccgcactcac
    10681 agaggttaga cgtgcatttc acactttaaa aggttctggc cgaatggttg gagcgaaagt
    10741 tgttggtgaa ttagcttggt caattgagaa tatgctcaat cgtgtcattg acaaaacgac
    10801 acaaccaaca gcagcaatga tgcagttgat tgctggtgtt gtagagcttg tcccgacctt
    10861 aatgaccaat ttccaagata agcaggtgcc aagttataaa acagatggtt taatgactgc
    10921 cgctgattta tttgccaaag ctcaatcttt atctagtgat gatgttcaat gggcaattga
    10981 ctatacatat aatcgcttag ttacatcgac atctgttgcc gatgtgatta tgcaagaaga
    11041 gccagagata gagttagcta ttgagcctgt ggttgtagca gacgtggagg aagatactga
    11101 tttaggaaat gtcacaattc ctgttatggc tgcgattaat gttcctgagc ctaaagatga
    11161 cttagagact caagaggatc aaagtgctga ggctatttct tttgaggatt ttgatgacga
    11221 aggcgtgttt gatgttccaa tggatatggg tgaagaaaca gatttaacgg ctgctccaga
    11281 attattacca caacaagaag ctgttgtgat tagtgaagta ccaaacgaag tgatatcaat
    11341 agctaaacct gcattaacta ttgatattcc tcaacatatt ccagtaccag aggatgactt
    11401 tagtcaggat gatgaaattc gtgaaatctt tattgaagaa gcacaagaag tattagaaac
    11461 aattaatgag tatttcccta aatgggcggc taaatttgat aacaaaaccg ctttaaccga
    11521 gtttcgtcgt ggttttcaca ccttaaaagg ctcaggccgc atggtgggtg ctaaagttga
    11581 aggtgagctg gcatggagca ttgaaaatat gcttaatcgt gttattgata atacgattaa
    11641 acccaatgca gaactagttg ctttaattag ccatgtgata gaggttattc cacaactgat
    11701 tactaacttc caaaataaag aagctcctag ctttaatagt tcgccaataa tgagcgttgc
    11761 tgataatctt gctcgtggca agcaagtaac aatggaggag ctaaatgcag caattgcctt
    11821 tgcacatgga caagtgcctg caacagtaga gcctgtagaa gaagtcgcat tagtacaaga
    11881 aatagcagta tcagactcag tagcgacaga ggactcacaa gattacagta atgcttttga
    11941 agatttagcg aaaacgctca gtgaaattaa agatgaaaca acggctnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnagat acagataagt ttattgctca tgttgcagag
    12121 gtgtctcaag cacctgctgc agatgatgat gaagttattg agtcagctgc cccaattaat
    12181 accgcaggta ttgtagctgg ctttttggat ttgggtttgg aaagcttaat ggatgcccct
    12241 tgggagctgg cagaatggtt agcttctgaa caaaaagttg aacatattca aaatttggta
    12301 aaagacttag agttattagc accagctgct aaagcagcaa ttgtgatgcc attagttgat
    12361 attgttaatc agttaacggt ggtatatcaa tttgtcttag aggatattga aaaagcattg
    12421 tctaatggcc agttgttaga agatctagca aatgctcatg acgaaatcat caatattttt
    12481 gataccttag cagcttgtca aactgttcag cctaatccca aattattaga gcgtttacgc
    12541 gaatggggtg gtagtcctgt cgattttgtt gatacagccg ctatacccag ttatgtgcca
    12601 gcatcccaag aaattgatga aggtgcagac ccagagttat tggccatttt cttggaagaa
    12661 gctgaagaag tattaactgc tgctgatgat gagttgattg cttggaaagc caatccagaa
    12721 gataagaatc ctttgcgagt attacaacgt catttgcaca ctttaaaagg tggtgcgcgt
    12781 atggctgttg tcactagttt aggtgatttg gcgcatgagt tagagttttt atatgaagat
    12841 ttggtcaatg gtcgttatca aggcacaaca gttttaatgg atttgctaca acgttgtcat
    12901 gaccgcttgg cagatatgat agatggctta aaggcagagg gacgttgtta ttacgccaaa
    12961 gatttagtac aggtaataga tatttatcgt cgtcaaccga ctgataatgt gattttggat
    13021 tttttctcct cagcggcagg tggttcgact aaacctgtag aagtgcctgt tgcaacatct
    13081 gccgcgcagc cgacacctgt tgcagagcaa cccgttaatg ttgttcagcc tactccacaa
    13141 gcagcagttg tgagcaatcg aaaagcagaa actgatgaag aattagatcc agaaatgtta
    13201 tctattttct tggaagaggc tcaagagatt gttgatgaaa ccaatgctag cttgcagcaa
    13261 tggatgcaac agccagaaga tgtgacacca gtacaaattt tgcaacgtgg tttgcacacc
    13321 ttaaaaggtg gtgcgcgtat gtcaggtgtt aattctttag gcgatttagc gcatgaaatg
    13381 gaaaatatct atgagggagt ttgtcaaaaa cgttatcaag gcaacaaaga ggtcttttct
    13441 gtactgcaac gttgtcatga tcgtattgca gaagtggttg ataatctaaa agatcaagga
    13501 cgtgctttag tagtgacaga cttggttgaa caattaaaac gttatttgtc taatcctcag
    13561 gcttttgttg acactgtgca aatggcagtg gctacaccaa ccgccgttac acctgttacc
    13621 acagacaatg atgtagtgcc tgtagcagtt gtagtagagg ctattgatga caatgtgatg
    13681 cccaaaatgt tgggtaactt ccgtgatgct gctaaaaatc agcaagcaca agaaatgatt
    13741 cgtgtttctt ctgaattaat ggaaaagcta attaacattg cgggtgaaac atcgattgtt
    13801 cgctcgcgtg ttgaaatggg ggtacatagc tttagccaaa ccgtagagga aatgggggct
    13861 acggtacagc gtttggctga acaattacgt cgtatgcagg gtgagcttga gtcacaaatt
    13921 cttgcccaac atacaacaga agacacaaaa tatgctgatt ttgacccctt ggagatggat
    13981 caatactcgt ccttaaatca gttatcaaaa tcattatatg agtctgcatc tgacttaatg
    14041 gatttgaaag gtacattaat cgacaaaaca cgtgatacag aaaccctatt attgcaacaa
    14101 tcgcgtttaa ataccgaaat gcaagaaggt ttaatgagtt ctcgtttagt tccattctca
    14161 cgtttggtgc cacgtttaca acgtatcgtg cgtcaaaccg caactgaatt acataaacca
    14221 gcggagttat atgtcgttaa tgcggaaggg gaaatggata gaaccttatt agaacgtatt
    14281 gttgcaccat tagagcatat gttaagaaac gcagttgacc acggtttaga aactccgcca
    14341 gaccgtgcgg cagcaggcaa agaagcgatg gggcgtatta cattatctct ctctcgtgag
    14401 ggcggtgaaa ttgtcttaac attagctgat gatggtcgtg gtgttaatgt tgaagccgtt
    14461 cgtaaaaaag ctattgagcg tggcttatta caagccgatg ccactatcag tgaaaaagag
    14521 ttattgcagt tcttattcca tgcaggtttc tcaaccgcgc agaaagtgac tcaaatttcg
    14581 ggtcgtggcg ttggtatgga tgtggtgcaa agtgagatta aacaattagg cggtacagtc
    14641 gtcattcaat ctgtcgctgg taaaggaaca acttttgtca tgcgtttacc attaacggtt
    14701 gcggtcagcc gtgccttgat ggttcgtatt ggtgaagatg tatatgcgat tcctttatcg
    14761 cagattgaag gtattgttcg tgctaatcct tatgagttag aaacttacta tgcgcccaat
    14821 gcacctgctt ttgaatatgc gaatattggg tataaactgc attatttagg tgagtttgta
    14881 catggaatcc gtgtaccgag tttatttggc cagaccttac cattaccgat attattggtg
    14941 cgtggagcgg atcagcgagt agccatacaa gttgaccaat taattggttc tcgtgaagtt
    15001 gttgtaaaat cagtcggagc acaattagcg tcagtagcag gtatttcagg tgcaaccatt
    15061 ttaggggatg gttcggttgt tatcatctta gatacgattg cgatgttgcg tgctgctgca
    15121 ctgcaaaaac cacgtcaacg tgtggcggaa gacgataccc cagctattat taaaccagag
    15181 cgtgcaacca aaatggttat ggtggttgac gactccgtaa cagtacgtaa agtaacatca
    15241 cgcttattag aacgtcatgg ttatgaagtt gttttggcta aagatggttt agatgcgatt
    15301 actaaattgg aagatgtacg tcctgatatt atgttgctcg acattgaaat gccgcgtatg
    15361 gacggtttcg aagttgcatc tttagtacgt cacaatccaa atttaatcgg tttaccgatt
    15421 attatgatta cctcacgtac aggtgaaaaa caccgtgaac gtgcattcca aattggggta
    15481 aatgcctata tgggtaaacc attccaagaa caacaattac tagagactat ttctgagtta
    15541 ttagtagcgg cttcgacaaa ataaggttta atcatggcga aacggaatcc taaagttgct
    15601 attgtgtcag atataagctt gcaaaggctt gccctcgcac atgcagtaaa agggcaaggg
    15661 tatgatttgg tattaaacag ctcgcctgaa cgattggatg atcgtttatt taaatctgtt
    15721 gagcctgatg tatggattgt tgatttacaa aatgaagatg atgagttatt agatcgtatt
    15781 ttagatttag gtgtgccagt actatttggc ttagatgctg cacctgctca aggaacacgg
    15841 caatatccac gttgggagcg tcgggttttt ataaaacttt atgacgttgt tgggcatcct
    15901 gttattcaag aaaatcttga accactagaa aaacaagcag ttgcgcctgt tgcaccacga
    15961 gagatttatg ttccacaaga attaatgacg tataaatctc aacgattgga gtttgtgtgt
    16021 gtgctagcag catcgttagg tgggccagca gcggttaaag agtttttaga ttttattcca
    16081 tcaggattgc ccgttgcttt tgttttagca cagcatattg atgcgcgtat gcaagagtct
    16141 ttaacacgtg ttttggtgcg acataatcat atgccttgtc atattgcaca tgatggagat
    16201 aagttgaaat caggtcagct attgattgcg cctgtagaaa cggaaataga ttttagtgct
    16261 gatggtcagg ttatttcacg taatattaaa tgggatggcc catattcacc ttctattgac
    16321 caagttttgg ccaatgttgg ccgacgtttt ggtaaaaaat ctattgcgat tatttttagt
    16381 ggtatgggta gtgatggttc gattagtggg ccacagatgg tagaagcggg tggttctgtt
    16441 tgggcgcaag atgaaaaaac ctgtgcttgt ccaagtcaac cagatgcgat gagagcaaca
    16501 ggatgtgtat cattttctgg aacaccattt gatttggcaa atcgtttagt aatgcatata
    16561 agtagaaatt tatcagcaca tacggcttag aagggttaaa acacatggca acacaagcaa
    16621 ttacactgag tcaaaccact ttgttgagtg caacaacggg taaattagat gtgtatatct
    16681 tgcctatttg gcaaaaaaaa ttactgttac caacggttgc tgttgttgat attttgccag
    16741 caagtcaatt aaaaacgact aaaaaaaata aaaaagttgg catattaggt caaattgttt
    16801 gggagcaaga aaccatccct gtgattgctt ttgaaacatt taatggagag tctcagacgg
    16861 cggagcctca aaaaattgcc attatggttg cggctacatt aggtgaatta caatattatg
    16921 gtttggcttt acaagcagaa ccaacaactg ccaaagtcaa aattgctgaa atcgaagatt
    16981 tagagcaagc tgcgatagga cctatggagt atttgcaaat tcgctatcaa aatcaactat
    17041 gtgttattcc agacttggat gcggtagagc aaaatttagc a
//
LOCUS       DAOQMB010000010        16701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1240_length_16701_cov_16.180524, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000010 DAOQMB010000000
VERSION     DAOQMB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 16701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16701
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_16701_cov_16.180524"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..563)
                     /locus_tag="PLY05_00920"
     CDS             complement(30..563)
                     /locus_tag="PLY05_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HNC01893.1"
                     /translation="MEIIRQHQLLDIILELHKSSLKGDFTAYKNHCYRVLNFYAAFVA
                     KTPDNLNKGAVAVAFHDLGIWTDNTLDYLPPSVNRAKTYLAEIQHSYWQDDVSLMIDN
                     HHKITPFHHNALVEGFRKADWLDVSLGLMNFGLNRQFISTIQREFPDAGFHLRLVQLS
                     LANTLKHPLRPLPIFKW"
     gene            640..6414
                     /locus_tag="PLY05_00925"
     CDS             640..6414
                     /locus_tag="PLY05_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HNC01894.1"
                     /translation="MRLLSAVILYIMSFSLFAFDLQQVVANAKATAYQVAGQTVALKT
                     FSPQGDVGSVGQARARFAEDMVRVGDKNVRNPFIVGAACKDYGRGRWLDTRTWVYDFN
                     NNLPVGTKCEFNLVAGLKSVAGKPVKAYPYYQFTVGDVTFGYDYSATQGVRVVDAWPY
                     HGNSILDDQVFLVKLDKPTDPRSLPSQVYCLTSDSPEKLYAKFFTIQETQAWLAKQDQ
                     ELKDWWRKNTSREYYDQQSNQYKTSTVKEWRALSCGRRLTGGQQVRLIWGKQVATATG
                     QKRTSDQILAYDVRQPFTVSFSCPRQSPKAPCVPLTNMELRFSEVITADKIKAIRLQG
                     SNQVWTPMPNSGEDYEDSDYVRFAAPFPANAAFLLSLPSGIEDVYKRPLTNAKNFPLQ
                     VKTGGYPPLAKFAADFGVIEKAVGAVPLTIRQLEPDMAAQTDAKLYSFKVADDDAAFV
                     SALKSYQNINNYGALDKPLIAKQAGVSVQPLPRKLTARDFEVIGIPVNTGLYIHEVES
                     RYLGEWISQKQQPAYVHTMNLVTNLGIHVQMGKSNSLVWVTDLAKGQPVANATVSVWD
                     CYNNKQLWTGQTGEQGTVKIAKDLFKYPKDTDNKTSCQNSPWLIMAKLGDDRSFALSE
                     WTNGIETWRFNLSSVDDYYYDEESDEYYGSSKSTGDFVAHTVFDRSLLRVGETVHMQH
                     LVRYAKLTGLSAPNKSTNINEVLIEHSGSGEQYTVSTKLSPIGNGESTWTIPKQAKLG
                     EYCVKLKKSADNYLHTGCFNVSAFRLPVLKANLGIASHTVANSSKQIPLQLQLRYLNG
                     GGYSNAPVTLRGRLEQSWLSLPNFEEYSFDSVPVMVNDKTQDNQDLEQQELVLDDSGS
                     LNALTPTLPDIKQVSRVQLEMEFRDPSGETQTVGASSTVWPAEVMVGSKVASSWVAKN
                     QPVEVDFISVNQQQQLQPNTPIKILGQMIRYESHRKRTLGGYYSYDTKTIKEPVTVNC
                     GNQTDAQGHLRCQFNFAKAGELELTAISTDKTGRQAATRTSLWIAGEDEWWFEQGDDD
                     RIDVLADKKDYKVGDVARLQVRMPFREATALVSVVRDGILESFVMPISGKDSVISVPI
                     KPEYAPNVFISATIVRGRNQQIQPTALVDLGKPAFKLGIAELKVGWEGFQLGVDVQTD
                     KSSYHPREQAQVTVKVSPSKGRDGLPNDTEVTLAVVDEALLELANNDSWNVLPSMMLS
                     RAYGIDNATNQLQVVGKRHFGKKALPTGGGGGRGGSTRELFDTLLLWQASTPVDSQGM
                     ARFSVPLNDSLTSFKLVAIASSREQFGTGSASMRNTQELQILSGLPSVVRDNDRYQAE
                     FTLRNSSPSPQTVQVTINSEVLKAPYQQSVVLAANGTQIINVPVVMTEGSQNVTWQVQ
                     AQSELYRDALKVSQKVLPAVPMQILSSELKQISAAKAYQGNLAALPAQALAGKVLLDV
                     QPNLGGTLEPVKQWFRSYPYICLEQKTTKATGLQDTSLWADIMGDLPTYLDKDGLAKF
                     YPSEGESSGSPFLTAHVLRAAKALNWQIPEDSRNAMLDGLLRYAEGKVNQEIYRYWIY
                     EPQFDVVQRHIEVANILAQYGRFKPNLLDNIRLQPSIWPNHILIDWYELLKNANNVPK
                     RAERLAEVERLLKAKLVQQGGTYIILKHDKNRWWWYDDDQVLQARLTLAVMGDKNWQD
                     ELPFLVRGLIRQQQQGHWQGTQSNLWGGFVLKRFNDNALSITGKTLAQLGTEQASTAW
                     PKATDANSQIVRFNDTENSDMSFAWPKQNSQITVNHQGEGSPWLRVSSAARIPITKAD
                     EKGYRIFKDIIPIQQKVKGEWHVGDVMRVELSFTSSQEMGWVVVNDPIPTGAVLLGRG
                     LKRDSELLNRGTNDWWPVYVEYAADAYRAYYQYLPSNTKWQSAYTVRLNQAGTFKLPA
                     TRVEAMYAPDVYGLSPNKTMEVKP"
     gene            6518..6600
                     /locus_tag="PLY05_00930"
     tRNA            6518..6600
                     /locus_tag="PLY05_00930"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6552..6554,aa:Leu,seq:tag)
     gene            complement(6784..9528)
                     /locus_tag="PLY05_00935"
     CDS             complement(6784..9528)
                     /locus_tag="PLY05_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HNC01895.1"
                     /translation="MAIWVTWPAWTKFGTLGIAAGLITLAAERSDLLKNNMFDLEDYP
                     KLNKNIVCDERSKTARTEDGTCNILSNPAEGSAYVRFGRNVNPAYTAGETEANTLLSP
                     NPREVSNTLLARGEFKPAPSLNFIAASWIQFMVHDWVDHGENQITNPIKVPLPAGDSM
                     GSGTLNVRRTQADPSRTAADAGKVNTYRNHNTHWWDGSQLYGSSKAQNDKVRSFVDGK
                     LKINADGTLPTELLSGKPITGFNENWWVGISMLHQIFTKEHNAIATMLKQKYPNATDQ
                     WLYDKARLVNSALMAKIHTVEWTPAVIANPVTERAMYANWWGLIGNANGRDKYQAETR
                     QWYEDLAKTDSWLKTFLGANTDLAGNVGSGTLDHAIAGLVGSANPNNYGVPYTLTEEF
                     VAVYRMHPLMRDKVDIYDIGSNVVSKSVPVENTRDRNAESMLTSESPDRLWYSFGITN
                     PGSLTLNNYPNFLRNLSIPVVGNIDLATTDILRDRERGVPRYNQFRRQIGLNPITKFE
                     DLTTEPAALANLKRVYSNDIEKIDTLVGMMAETTRPDGFAFGETAFQIFILNASRRLM
                     TDRFFTKDYTPTVYTPEGYAWVENTTMVDVIKRHHPQLAASLVGMENAFKPWGLNMPA
                     DYEKWSAQAKADHLWVNGAKRTQYKTTMPNLTKVDTAGLISSILWKKVQSRTDVAPAG
                     YNKPIHAHGAMAKVKFIPVANNGYTGLFQGSNYGLLRLSVTENPAEGAFQTGLAWKAL
                     VDGKPSENVSALYTLAGQGQNYNFFANELSNYVSPDINNSASTILFSAVSTKPTLVVM
                     NDMAEVMQNGTVVTTPKAPTQIYFVPRPEVKTKFSTAAHDFRYDLATLGAGSKLYDVY
                     ATSMEIKTSIIPSTNLSYAQQRRASAKKIGELELTSPLIVSAFGDSGVFFKHQRYEDR
                     "
     gene            10161..11855
                     /locus_tag="PLY05_00940"
     CDS             10161..11855
                     /locus_tag="PLY05_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005029930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC01896.1"
                     /translation="MTANAEKQTLLHCLLYWEKNTPSGIYLTQPFGNGVVKDYTWQQV
                     GNEVRRIAAYIQSLNLPPKSNIALLGRNSAHWIMADLAIWMSGHVTVPLYPTLNGETA
                     EYIFEHSEAKLLIVGKMDGKADGWKEIKTVIPQGLPIIAAPLSPPELSNQPQWDDLIA
                     KNEPLKNPTMPQLDDIATIVYTSGSTGKPKGVLHSFRTMVVITSGMEEIWGFTSSERM
                     LSYLPLAHVAERAAVETMSLYFGFHVFFSEGLETFQKDLQRAQPTIFFSVPRLWTKFY
                     LGVNEKIPAQKQKILFNIPILNKIIKKKVLTQLGMNHVRVALTGSAPLPPQIIEWYRN
                     LGLELLDVYGMSENFAYSHACKPNEVRVGYVGSVNPGVLHRIADNGEIQVKSPAQMIG
                     YYKMPDKMAEEMTDDGYFKTGDRGEIDEKGRLRITGRVKELFKTSKGKYVAPVPIENK
                     LNNHPKVEVVCVTGAGEPQPFALFMLSLDAIKELEKGELDKETLTNEIKELLKSVNST
                     LEDHEVLDYVVIVKDQWTMENGFLTPTMKIKRNVIEDRYLKHGEQWVAQKQKVIWE"
     gene            11937..>13690
                     /gene="pbpC"
                     /locus_tag="PLY05_00945"
     CDS             11937..>13690
                     /gene="pbpC"
                     /locus_tag="PLY05_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HNC01897.1"
                     /translation="MLRSLWIVGLWLLIAIAQASQPPTFSQIQQQWQSSYGRLLDRHG
                     QVLAYQRLNKQQHRFEWTPLEEVSPALIDALLFVEDRDFYRHHGVDWGAVSRSAVSYL
                     TGQKARGASTLTMQLTAMLDPKLSWQTGGRSLKRKWQQLQAARQLEKTWTKAQILEAY
                     INLMVWRGDLQGVSTASLALFGKWPRGLNKEESILLVSLLAAPNASIQRIADRACVLI
                     NAGYQADCQEVKELAFQQLSQRRLPIEQRADSLSLAARLLSQANQIYQTSLDKNLQDF
                     SYQALRSQLASLAAQKANAGAVLVLNNQTGEVLAYVANSGLVEDKVWVDGIYAPRQAG
                     STLKPFLYGLAFENNLLTAASVLDDQALNISTEQGLYVPQNYEKDFKGAVIARRALAS
                     SLNIPAVLTLNKVGLENFWKMLNELGFSLEQQADYYGNALALGGADINVWQLANAYRA
                     LANQGQFSEATLELSPPTSPTKTILSPQASFIVADILSDRNARSLTFGYENPLATPFW
                     SAVKTGTSKDMRDNWCVGFSRRFTVAVWVGNMQGLPMRDVSGITGAAPVWQLVMNYLE
                     AGAGQSFAPQPPKGVHQQ"
     assembly_gap    13691..13790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13955..14716
                     /gene="pgeF"
                     /locus_tag="PLY05_00950"
     CDS             13955..14716
                     /gene="pgeF"
                     /locus_tag="PLY05_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HNC01898.1"
                     /translation="MSLKDSFIKPEWPAPTNVCAVVTTRIGGCSDAPYDSFNLAFHVG
                     DEPSKVVENRRKLLIALNEIAPCGPPQWLQQVHGTDVVDAFSEPKRRAQTVPEADAVT
                     TTQRGLPCVVMTADCLPVFFCDQSGHRVAVAHAGWRGLSNGILENTLAKFLNPAEVMC
                     WLGPAIGTTAFEVGADVRDAFLRVDPAAESAFTVKANGKYLADIYDLARQRLMKAGIG
                     MISGGNLCTVTDQERFFSYRREQTTGRMASVIWLA"
     gene            14716..15264
                     /locus_tag="PLY05_00955"
     CDS             14716..15264
                     /locus_tag="PLY05_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNC01899.1"
                     /translation="MAKNIVIVYHSGYGHTAKLAEYIALGVAQIANVDSYLLSVDNID
                     WQILANADAIVFGCPTYMGCVSAPFKTFMDDTSKVWLEQSWQGKLAAGFTNSGGLSGD
                     KLAVLQQIQLFAMQHGMLWAGMPLMPTGTQPEDLNRMGSYIGLMAQSDSAPVDVTPPV
                     GDLRTAEWFGQHIAKTVQRIIN"
     gene            15311..15526
                     /locus_tag="PLY05_00960"
     CDS             15311..15526
                     /locus_tag="PLY05_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01900.1"
                     /translation="MYEQPQHLPFLQAIAWQHSAVTALTEDEILSLYERGWHYRGVLA
                     DLTGEELAFVRKLATEKKSWIVIDVCP"
     gene            15513..16268
                     /locus_tag="PLY05_00965"
     CDS             15513..16268
                     /locus_tag="PLY05_00965"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC01901.1"
                     /translation="MFALEFHQKISQVLLALNVGFFQKASAYFGGGTLLALAYNEYRW
                     SKDIDFVCPVGEHYRYLRSKIMEEGYQAIFQNVQGIQLPREIKADQYGIRFPVVIDNT
                     LIKFEIIAEARILLESPVYLNWLNIPCLSFVDRCCEKLLANADRWNDTAVESRDLIDL
                     AMLRLASHIPQLAFDKAEAAYDVKKPLFRALNNFQQKPEYRQKCFDALHISKKALIID
                     GIDALALDLGLLATQRTEQEMHLDEYGLPRDMT"
     gene            16265..16579
                     /locus_tag="PLY05_00970"
     CDS             16265..16579
                     /locus_tag="PLY05_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC01902.1"
                     /translation="MITHLTITDFQPQATDIIWDVRDAKAYAEGHWQPAQHQALESIS
                     LATLQTLPSEQTIYVLCGGGTKAGRAAQLIEELDKQRQVVILTGGTRAAKAAGLPMVE
                     GA"
BASE COUNT         4896 a         3107 c         3752 g         4846 t
ORIGIN      
        1 ggtatgattt aggcgcgtat tgcgcctgtt taccacttaa aaatcggcaa aggccttaat
       61 ggatgtttta aggtattagc caaagagagt tgcactaagc gtaaatgaaa acctgcatca
      121 ggaaattcgc gttgaatggt gctaataaac tgtcgattta aaccaaaatt catcaatccc
      181 aaagacacat ctagccagtc agcctttcta aatccttcga ctaaagcatt atgatgaaat
      241 ggcgtaattt tatgatgatt atcaatcatt aagctcacat cgtcttgcca atatgagtgt
      301 tgaatttcag ctaaataagt tttggcacga ttaacggagg gtggcagata atctagggtg
      361 ttatctgtcc aaatgcctaa atcatgaaaa gcgactgcaa cagcaccttt attgagattg
      421 tctggtgttt tggcgacaaa tgcagcataa aaattaagca cgcgataaca atgattttta
      481 taagctgtaa aatctccttt tagtgaagat ttgtgaagct ctaaaataat atctaatagt
      541 tgatgttggc gaataatttc catctttgtt cctatagcct ttgtactata ctaagtcagt
      601 ttatgtttct tgaaatttgt taattgcaaa ggaggcgata tgcgcctgtt gtctgccgtt
      661 attctttata tcatgagttt tagcttgttt gcttttgatt tgcaacaagt cgttgccaat
      721 gccaaagcaa cggcttatca agtggcaggt caaacggttg cactcaaaac ctttagtcca
      781 cagggtgatg ttggctcggt ggggcaggca agagcacgct ttgctgaaga catggtgcga
      841 gtgggcgaca aaaatgtgcg taatcctttt attgtcggtg cagcgtgtaa agattatggc
      901 cgtggtcgtt ggttagacac gcgtacatgg gtttatgatt ttaataataa tttacctgtg
      961 ggtactaaat gcgaatttaa tttggttgca ggtttaaaaa gtgttgcagg gaaacccgtt
     1021 aaggcctatc cctattatca gtttaccgtc ggggatgtta cctttggtta tgattatagt
     1081 gcgacacagg gtgtgcgtgt ggttgatgcg tggccatatc atggcaatag tattttggat
     1141 gaccaagtat ttttggtgaa attagataaa ccaaccgatc cgcgttcttt gcctagtcag
     1201 gtgtattgtt taaccagtga ttcccctgaa aaattgtatg ccaaattttt taccattcaa
     1261 gaaacccaag catggttagc caaacaagac caagagctga aagactggtg gcgaaaaaat
     1321 actagtcgcg aatattacga tcaacaaagc aatcaatata aaaccagcac cgttaaagag
     1381 tggcgtgcct taagttgtgg ccgtcgttta acaggtggcc aacaagtacg tttaatttgg
     1441 ggtaaacaag tggccactgc aacaggccaa aaacgtacta gcgaccaaat tttggcctat
     1501 gatgtccgtc agccctttac cgtcagtttt agctgccctc gacaaagtcc caaagcaccg
     1561 tgtgtgccat taaccaatat ggagttacgg tttagcgaag tgattacggc ggacaaaatt
     1621 aaagcgattc gtttgcaagg gagtaatcag gtatggacac ccatgcccaa cagtggtgag
     1681 gattatgaag acagcgatta tgtccgtttt gctgcaccat ttcctgccaa tgctgcgttt
     1741 ttattaagct tgccttcagg cattgaagat gtttacaaac ggccattaac caatgcaaaa
     1801 aattttcctt tacaagtaaa aacgggcggt tatccaccgt tagccaagtt tgctgctgat
     1861 tttggcgtga ttgaaaaagc agtaggagct gtgccgttaa cgattcgcca attagagcca
     1921 gatatggccg cgcaaactga tgccaaactg tatagtttta aagtggcgga tgacgatgcc
     1981 gcttttgtta gtgctttaaa aagttaccaa aacattaata attatggtgc tttagataag
     2041 ccgctaattg ccaaacaagc aggagtttcg gttcagccct taccacgaaa attaaccgct
     2101 cgtgattttg aagtgattgg catacctgtg aacacaggtt tgtatattca cgaagtggaa
     2161 agccgttatt tgggcgaatg gattagccaa aaacaacaac ccgcttatgt acataccatg
     2221 aacttagtga ccaatttagg cattcatgtg caaatgggca aaagtaatag tttggtatgg
     2281 gtcactgatt tagccaaagg tcagcctgtt gctaatgcaa cggtgagtgt ttgggattgt
     2341 tataacaata aacaattgtg gacaggccaa acgggtgagc aaggtacggt taaaattgcc
     2401 aaagatttat ttaaatatcc taaagatacc gataataaaa ccagttgcca aaattcaccg
     2461 tggttgatta tggcgaaact gggagatgat cgtagttttg cactctctga atggacaaac
     2521 ggcattgaaa catggcgttt taatttaagc agtgttgacg attattatta tgatgaagaa
     2581 agtgatgaat attatggctc aagcaaatcc acaggtgatt ttgttgccca taccgttttt
     2641 gatcgttctt tattgcgtgt tggtgaaacg gtgcatatgc aacatttagt gagatatgcc
     2701 aaattaacag gtttaagtgc gcccaataaa agcaccaata tcaatgaggt gctaattgag
     2761 cattcgggca gtggtgagca atataccgtg agcaccaagc ttagtccgat tggtaatggt
     2821 gaaagcacat ggacgattcc taaacaagcc aaactcggcg aatactgcgt taaattaaag
     2881 aaaagtgccg acaactattt gcatacaggc tgttttaatg tctcagcgtt tagattgcct
     2941 gtattaaaag ccaatttggg cattgcaagt catacggtag ccaatagtag taaacaaatt
     3001 cccttacagt tgcaattacg ctatttaaac ggcggtggtt atagcaatgc gcctgttaca
     3061 cttcgtggtc gcttagagca gtcttggtta tcgttaccga attttgagga atatagtttt
     3121 gattctgtgc cagtcatggt caatgacaaa acacaagaca atcaagattt agagcaacaa
     3181 gaattggtgt tggatgactc aggcagttta aatgctttaa cgcctacatt gcctgacatt
     3241 aagcaagtga gtcgtgtgca gttagaaatg gagtttagag accccagtgg tgaaacacaa
     3301 acagtggggg caagtagcac cgtttggcca gccgaagtca tggtcggttc aaaagtggct
     3361 tcgtcttggg tggcaaaaaa tcagcctgtt gaagtcgatt ttattagtgt gaatcagcaa
     3421 caacaattgc aacccaatac ccccattaaa attcttggtc agatgattcg ttatgaaagc
     3481 catcgtaaac gtactttggg tggttattat tcttatgaca ccaaaaccat taaagagcca
     3541 gtcacagtca attgtggcaa tcaaaccgat gctcaaggcc atttacgttg tcagtttaat
     3601 tttgccaaag caggtgagtt ggagttaacg gccatcagca ccgataaaac aggccgtcag
     3661 gcagcaacac gcaccagttt atggatagca ggtgaagatg agtggtggtt tgagcaaggc
     3721 gatgatgatc gtattgatgt tttagccgac aaaaaagatt ataaagttgg tgatgttgca
     3781 cgcttacaag tgcgtatgcc attccgtgaa gcaaccgctt tagtgtcggt ggtgcgtgat
     3841 ggcattttag aaagttttgt catgccaatt tcgggtaaag attcggtgat ttctgtgcca
     3901 attaaacccg aatacgcacc taacgtcttt atttcagcaa ccattgttcg tggtcgtaat
     3961 caacaaattc aaccaactgc gttagtggat ttgggtaaac ctgcctttaa attgggaatt
     4021 gcggagttaa aagtgggttg ggagggtttt caattaggcg ttgacgtgca gaccgacaaa
     4081 agcagttatc acccacgtga acaggctcaa gtcaccgtta aagtcagccc tagtaaaggt
     4141 cgtgatggct tgcccaatga caccgaagtg accttggctg tcgttgatga agctttattg
     4201 gaactagcga ataatgactc atggaatgtg ttgccgtcga tgatgttaag tcgggcttat
     4261 ggtattgaca atgcaaccaa ccaactacaa gtagtgggca aacgtcactt tggtaaaaag
     4321 gctttaccaa caggcggtgg cggtggtcga ggtggttcaa cacgcgagtt gtttgatacc
     4381 ttgttgttgt ggcaagccag tacccctgtt gatagtcaag gcatggcacg ttttagcgta
     4441 ccgctgaatg attcattaac cagttttaaa ttggtggcga ttgccagttc tcgtgagcag
     4501 tttggcacag gctcagccag catgagaaac acccaagagt tgcaaattct cagtggtttg
     4561 ccgagtgtgg tacgtgataa tgaccgttat caagccgaat ttaccttgcg taatagcagt
     4621 cctagtccac aaacggtgca agtgacgatt aatagtgagg tattaaaagc gccttatcaa
     4681 caatcggtag tattggctgc gaatggtaca caaatcatta atgtgcctgt ggtgatgact
     4741 gaaggaagtc agaatgtcac atggcaagta caagcacaaa gcgaattgta tcgtgatgcc
     4801 ttaaaagtca gtcaaaaagt tttgcctgct gttcctatgc aaattttaag ttcggaatta
     4861 aaacaaattt cagccgcaaa agcatatcaa ggaaacttgg cagcattacc agcacaagct
     4921 ttagcaggaa aagtgttgtt ggatgttcaa cccaatttgg ggggaacttt agagccagtc
     4981 aaacaatggt ttagaagtta tccttatatt tgtttagaac aaaaaaccac taaagcaaca
     5041 ggcttacaag ataccagttt gtgggctgat attatggggg atttgccaac ttatttggat
     5101 aaagacggtt tagccaagtt ttatcccagt gagggcgagt catcgggttc accgttctta
     5161 acagcacatg tgttacgtgc ggccaaggca ttaaattggc aaattcctga agacagccgt
     5221 aacgcgatgt tggatggttt attgcgttac gctgaaggca aagttaacca agagatttat
     5281 cgttattgga tttatgagcc acaatttgat gtggttcaac gacacatcga agtggcgaat
     5341 atcttggcac aatatggtcg ttttaaaccc aacttgttgg ataatattcg tttgcaaccg
     5401 agcatctggc ctaatcatat tttaattgat tggtatgagt tgttaaaaaa tgccaataat
     5461 gtccctaaac gtgctgagcg tttagccgaa gtagagcggt tattaaaagc caaattagtc
     5521 caacaaggtg gcacttatat cattcttaaa catgataaaa accgttggtg gtggtatgac
     5581 gatgaccaag tgttgcaagc gcgtttaacc ttagcggtga tgggcgacaa aaattggcaa
     5641 gatgaattgc cgttcttggt gcgtggttta attcgtcaac agcaacaagg tcattggcaa
     5701 ggtacacaaa gtaatttgtg gggcggtttt gtgttaaaac gttttaatga taatgccttg
     5761 agtattacag gtaaaacgct tgctcaatta ggcacagaac aagccagtac agcatggcca
     5821 aaagctaccg acgctaattc tcaaatagta cgttttaatg ataccgaaaa cagtgatatg
     5881 tcttttgcgt ggccaaaaca aaatagccaa attacggtta atcatcaagg cgagggtagt
     5941 ccgtggttgc gagtatcaag cgcagcgcgt attccaatta ccaaagccga tgaaaaaggc
     6001 tatcgtattt ttaaagacat cattcctatt caacaaaaag tcaaaggtga gtggcatgtt
     6061 ggtgatgtta tgcgcgttga gctgtcattt acctctagcc aagaaatggg ttgggtggtc
     6121 gttaatgacc ccattcctac aggtgcagtg ttgttgggac gtggtttaaa acgtgatagt
     6181 gagttgttaa atcggggtac aaatgattgg tggccagttt atgtggaata tgcggcggat
     6241 gcttatcggg cgtattatca atatttacca tcgaatacta aatggcaaag tgcttatacg
     6301 gtgcgtctca atcaagcagg tacatttaag ctgcctgcta cacgcgtaga ggcgatgtat
     6361 gcacctgatg tgtatggttt aagtcctaat aaaacgatgg aggttaaacc gtaacggata
     6421 gctggccaag gattggctgt cagcatactt ataacggaga gatgcattct aaaaaatctt
     6481 tgaattttat tgtttttatt gtaagatagt ccaccttgcg ccagtggcga aattggtaga
     6541 cgcgctggat ttagattcca gtccgtgagg gtgtgggttc gagtccctcc tggcgcacca
     6601 tatttacttc tccaaagaag ttttaaaagc ctcaacttta aaaaagtctg aggctttttt
     6661 gtgtctgaaa aataaataat aatcgaatac ttcgcccaaa ttagccaata ttttttgact
     6721 gcataaaaaa agccaacaca atgtgttggc tttttgcaca ataaacaagc tgtttataca
     6781 gacttatcta tcttcataac gttgatgttt aaagaacaca ccgctgtcac caaaggcgga
     6841 gacaattaat ggtgaggtta actctaattc accaattttc ttcgcactgg cacgacgttg
     6901 ttgtgcataa cttaaattgg tagatggaat aatagaagtt ttaatttcca ttgaagttgc
     6961 gtaaacatca tagagttttg aacctgcacc taaagtcgct aagtcataac ggaagtcatg
     7021 agcagctgta gaaaacttgg tttttacttc tggacgtggc acaaaataaa tttgtgttgg
     7081 tgctttaggt gttgtaacaa cagtaccatt ttgcatcacc tcagccatat cattcattac
     7141 cactaaagtg ggtttagttg aaacagccga gaataaaatt gtactagcag agttattaat
     7201 gtctggagac acgtagttag ataactcatt ggcaaagaaa ttataatttt ggccttgtcc
     7261 tgctaaagta tataacgcag atacgttttc agacggtttg ccatcaacta aagctttcca
     7321 tgctaaacct gtttggaatg caccttcggc tggattttcg gtaacggata ggcgtaacaa
     7381 accatagtta gagccttgga ataaaccagt ataaccatta ttggcaacag gaataaattt
     7441 cactttggcc atagcaccat gagcatgaat tggcttgtta taaccagcag gggcaacatc
     7501 agtacggctt tgcacttttt tccacaaaat agagctaatt aaacccgcag tatccacttt
     7561 ggttaaattt ggcatcgtgg ttttgtattg ggtgcgtttt gcaccattca cccataaatg
     7621 atcggctttg gcttgagcag accatttttc gtagtcagca ggcatattta agccccaagg
     7681 tttaaaggca ttttccatac ctactaaact agccgctaac tgaggatggt gacgtttaat
     7741 cacatcaacc atggtagtgt tttctaccca agcataacct tctggagtat aaacggttgg
     7801 cgtatagtct ttagtaaaga aacggtcggt cattaaacga cgtgaagcat ttaaaataaa
     7861 aatttggaat gcggtttcac caaaagcaaa gccatcagga cgtgtggttt cggccatcat
     7921 ccccactaaa gtatcaatct tttcaatgtc gttgctatag acacgtttta agttagctaa
     7981 agcggctggt tctgtggtta agtcttcaaa tttagtaatc ggatttaagc caatttgacg
     8041 gcggaattga ttgtaacgcg gtacaccacg ttcacggtca cgtaaaatat cggtggtggc
     8101 taagtcaata ttgcccacca caggaataga taaattacgc aagaagtttg gatagttgtt
     8161 taaggttaac gaaccggggt tggtaatacc aaaagagtac cataaacgat ctgggctttc
     8221 gctggttaac atggactcgg cattacgatc acgagtgttt tcgacaggca ctgacttgct
     8281 aacgacgtta gagccaatat cataaatatc gactttatcg cgcattaatg ggtgcatacg
     8341 atataccgca acaaactctt cagttaaggt ataaggcaca ccataattgt taggattagc
     8401 agagccgacc aaacctgcaa tggcgtggtc taaggtacca ctgcccacat tgcctgctaa
     8461 atcggtattt gcacctaaga aggttttaag ccatgagtca gtttttgcta aatcttcata
     8521 ccattgacgt gtttcggctt gatatttgtc acggccattg gcattaccaa ttaaacccca
     8581 ccagtttgcg tacatagcac gttcggtaac tgggttggca atcaccgcag gtgtccattc
     8641 aacggtgtga attttggcca ttaatgccga gttaactaaa cgagctttgt catataacca
     8701 ttgatcggta gcatttggat acttttgttt gagcatggta gcaatggcat tatgctcttt
     8761 ggtgaaaatt tgatggagca tactaatccc cacccaccag ttttcgttaa agccagtaat
     8821 cggtttgccg cttaataact cagtcggtaa tgtgccatcg gcattaattt ttaatttacc
     8881 atcaacaaaa gagcgtactt tgtcattttg ggctttgctg ctaccgtata attgtgagcc
     8941 gtcccaccag tgagtattgt ggttacggta ggtattgact ttgcctgcat cggcggcagt
     9001 gcggcttggg tctgcctgag tgcggcgtac atttaatgtg ccactgccca tgctatcacc
     9061 cgcaggtaat ggtactttaa ttgggtttgt gatttgattt tcgccgtggt caacccagtc
     9121 atgcaccata aactgaatcc atgaggcggc gataaagttc aggctaggcg caggtttaaa
     9181 ttcaccgcga gctaacaggg tattactgac ttcgcgtggg tttggactta ataaggtatt
     9241 ggcttcggtt tcgcctgccg tataagcagg gttcacatta cgaccaaaac gcacataagc
     9301 ggatccttcg gcaggattgc ttaaaatatt acacgtccca tcttcggtac gcgctgtttt
     9361 actgcgttca tcacaaacaa tatttttatt gagtttgggg taatcctcca aatcaaacat
     9421 attgtttttt aatagatcac tacgctctgc ggctaaagta attaacccag cagcgatacc
     9481 caaagtgcca aactttgtcc aagctggcca tgtcacccaa attgccattt ctgtgtcctc
     9541 atggtgttat ttaggactaa gtcgtggtga ttgcacgaag tccccgtgtg gtgtttcttt
     9601 ttttagaatt tgagagtggc tgaattagtg atagtcatct ttggagcgag agtagaacca
     9661 ttgacttaaa cactaagatc atcttcagtc ggcgcgcagt ctagcaaacc tgaatttcta
     9721 tcgaactatg ctaaaggagg gggagtggga ggggaaaata gtattttttg taaaaaaatg
     9781 acataaaaaa atatattttg accataatca attcataaac tttatgaaaa gtcatagaca
     9841 actacatttg ttagacaaac aaggcttttc agcaagtgcg gtcaatggta gtcttcacgc
     9901 cattgtgtga tgataaatta attaagcaga gatagttgtc tagggcaaca ttactgcaac
     9961 aatagcagca aacgttttag tctaaatagt ttgatgttaa aaaagatggt ctattgtaca
    10021 acgatagaat caaacaattg ccaagcctaa acgccattat gcttgttacc cattgacgtt
    10081 tagccctttt tgtattgcct taaacagaaa ataaatctgt gttgagcata atacgttgtg
    10141 atttatgagg atataacaac atgacagcaa atgccgaaaa gcaaactctt cttcattgtt
    10201 tactctactg ggaaaaaaat acgccaagtg ggatttattt aacccagcca tttggtaatg
    10261 gtgttgttaa agattacacg tggcaacaag tgggtaacga agttagacgc attgctgcct
    10321 atattcagtc gttaaattta ccacccaaaa gtaatattgc tttattaggt cgcaactcag
    10381 cccattggat tatggctgac ttagcgattt ggatgtctgg tcatgtgact gtaccgttat
    10441 atccaacctt aaacggtgaa actgccgaat atatttttga acacagcgaa gccaaattat
    10501 taatcgttgg taaaatggat ggcaaggccg atggttggaa agaaatcaaa accgtcattc
    10561 ctcaaggttt accgattatt gctgcaccgt tgtctccacc agaattatca aatcagccac
    10621 aatgggatga tttgattgct aaaaatgaac cgttaaaaaa tccaaccatg ccacagttag
    10681 acgatattgc aactatcgtt tatacctcag gtagtacagg taagcccaaa ggtgtattac
    10741 acagcttccg cacaatggtt gtgattacca gcggtatgga agaaatctgg ggcttcacca
    10801 gcagtgagcg tatgttgtca tacttgcctt tagctcacgt tgccgagcgt gctgctgtcg
    10861 aaactatgtc tttatatttc ggttttcatg ttttcttctc cgagggttta gagaccttcc
    10921 aaaaagacct acaacgcgct caaccaacga ttttcttctc tgtacctcgt ttgtggacga
    10981 agttctattt gggtgtaaat gaaaagattc ctgcacaaaa gcaaaaaata ttattcaata
    11041 ttccaatctt aaacaaaatc atcaagaaga aggttttaac tcagttaggc atgaaccacg
    11101 tacgtgttgc cttaacaggt tctgcaccgt taccaccaca aattattgaa tggtatcgta
    11161 atttaggttt agagttattg gacgtatatg gtatgtccga aaactttgcc tactctcatg
    11221 cttgtaagcc aaatgaagtg cgcgtcggtt atgtgggttc agttaaccct ggtgtattac
    11281 atcgtattgc tgacaatggc gaaattcagg taaaaagccc agcacaaatg attggctact
    11341 acaaaatgcc agacaaaatg gccgaagaaa tgaccgatga tggttacttt aaaacaggtg
    11401 accgtggtga aattgacgaa aaaggtcgtt tgcgcattac aggtcgtgtt aaagagttgt
    11461 ttaagaccag taagggtaaa tatgttgcac cagtaccgat tgaaaacaaa ctcaacaacc
    11521 atccaaaagt ggaagtggtg tgtgtgacag gtgcaggtga gcctcaacct tttgccttgt
    11581 ttatgttgtc tttagatgcc atcaaagagt tagaaaaagg tgaattggat aaagaaacct
    11641 taactaatga aattaaagag ttgttgaaaa gcgtaaacag cacgttagaa gaccatgaag
    11701 tgttggatta tgtggtgatc gtgaaagatc aatggacgat ggaaaatggc ttcttaaccc
    11761 caaccatgaa aatcaaacgt aatgtcattg aagatcgtta cttaaaacat ggtgagcaat
    11821 gggttgccca aaaacaaaaa gttatttggg agtaaattta acgcattcaa tttaatgtgt
    11881 taaaaatata aactaagggg caattatttt gccccttagt tttttaggaa tatgacatgt
    11941 taaggagttt atggattgtt ggattatggt tgttgatagc aatagctcaa gcctctcaac
    12001 cgccaacatt ttcccaaatc caacaacaat ggcaatcttc ttatggccgc ttattagatc
    12061 gacatggcca agtattagct tatcaacgtc tgaataaaca acaacatcgc tttgaatgga
    12121 cgccattaga agaagtatca cctgcgttaa ttgatgcttt attatttgtc gaagatcgtg
    12181 atttttatcg tcatcatggt gttgattggg gcgcggtaag tcgttcggca gtcagctact
    12241 taacaggtca aaaagcgcgt ggcgcaagta ccttaaccat gcaattaacc gccatgcttg
    12301 accctaagtt atcatggcaa acaggtggtc gtagtcttaa acgtaaatgg caacaactcc
    12361 aagccgcgcg tcagctcgaa aaaacatgga caaaagcaca aatccttgaa gcctatatca
    12421 atttaatggt gtggcgtggc gatttacaag gtgtcagcac cgctagtttg gccttgtttg
    12481 gcaaatggcc gcgtggttta aacaaagaag aaagcatttt attagtcagt ttgttggcag
    12541 cacctaatgc cagtattcaa cggattgccg atcgtgcttg tgtgttaatc aatgcgggtt
    12601 atcaagccga ttgccaagag gtcaaagaat tagcttttca acaactcagc caacggcgct
    12661 tacctataga acaacgtgct gatagtttaa gtttggctgc acgtttatta agccaagcca
    12721 atcaaattta tcaaactagc ctagataaaa acttgcaaga ctttagttat caagccttac
    12781 gtagccagtt agcctcatta gctgcacaaa aagctaatgc aggggcagta ttggtgctta
    12841 ataatcaaac aggtgaagtg ttggcttatg tggctaatag tggtttggtt gaagataaag
    12901 tttgggtgga cggaatttat gcgccacgcc aagcaggttc aacactcaaa ccctttttgt
    12961 atggtttggc gtttgaaaat aacttattaa cggctgcttc tgtgttagat gaccaagcct
    13021 taaatatcag caccgaacaa ggtttatatg tgccacaaaa ttacgaaaaa gactttaaag
    13081 gtgcagtgat tgcccgccgt gctttagcca gttcattaaa tattcctgcc gtgttaacac
    13141 tcaataaagt tggtttagaa aatttttgga agatgctcaa tgaacttggt ttttcgttgg
    13201 aacaacaagc cgattattat ggcaatgcct tagccttggg tggcgcagat attaatgtgt
    13261 ggcaattagc caatgcctat cgtgccttag ccaatcaagg acaatttagc gaggcaactt
    13321 tagaattatc ccctccaacc agtccaacaa aaacgatttt atcgcctcaa gccagtttta
    13381 ttgtggcaga tattctgagt gatcgaaatg cacgcagttt aacctttggt tatgaaaatc
    13441 ccttagccac gccattttgg tcggcggtca aaacaggtac aagcaaagat atgcgtgata
    13501 actggtgtgt ggggttttca cgtcgtttta cggtagcggt gtgggtgggc aatatgcaag
    13561 gtttacccat gagagatgta tcgggtatta caggtgcagc acctgtttgg caactggtga
    13621 tgaattattt agaagcagga gcaggacaat catttgcacc acagccccct aaaggcgtac
    13681 atcaacaatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tttattgtcc
    13801 gacaaaaccg tgattttaat ttataattcg tgctagtttt gcaaattaag tagttattat
    13861 ttctgtatgt gatgcttaaa aagccacaat tctctttatc ccaaccttaa agttctctaa
    13921 tgagtaagta tctatactct aaaggaatct tcctatgagt ttaaaagact cttttattaa
    13981 gccagaatgg ccagcaccaa ccaatgtttg tgcagtggta acgacacgta taggtggttg
    14041 tagtgacgct ccctatgata gttttaattt ggcctttcat gtgggggatg agccaagcaa
    14101 agttgtagaa aatcgtcgta agttattaat tgccttaaat gaaattgccc cttgtggccc
    14161 acctcaatgg ttgcaacaag tacatggaac ggacgtggta gatgcattta gtgagcctaa
    14221 acgccgcgcc caaactgtac ccgaagccga tgcggtgact accactcaac gtggtttgcc
    14281 ttgtgtggtg atgacggcag attgtttgcc agtattcttt tgtgatcaaa gtgggcatcg
    14341 tgttgccgtc gctcatgcag gttggcgtgg tttgtccaat ggtattttag aaaatacttt
    14401 ggcaaaattc cttaatcctg ctgaagttat gtgttggtta gggcctgcca ttggcacaac
    14461 agcgtttgag gtcggtgctg atgtacgtga tgctttcttg cgtgttgatc ctgccgccga
    14521 aagtgcattt accgttaaag ccaatggtaa atatttagca gatatttatg atttagcccg
    14581 tcaacgttta atgaaagcag gtattggcat gattagtggt gggaatttat gtaccgttac
    14641 tgatcaagaa agattttttt cttatcgtcg agagcaaaca acaggacgta tggcttcggt
    14701 tatttggtta gcataatggc aaaaaatatc gttattgttt atcacagtgg ttatggccat
    14761 accgccaaat tagccgaata tattgcttta ggtgtagctc aaattgccaa tgttgatagc
    14821 tatttgttat cggttgacaa tattgattgg caaatattag ccaatgccga tgccattgtt
    14881 tttggttgcc caacttatat gggctgcgtt tcagctccct ttaaaacctt tatggatgac
    14941 acctcaaagg tgtggttaga gcaaagttgg caaggaaaac tagccgcagg ttttaccaat
    15001 tcgggcggtt taagtggtga taaactggca gttttgcaac aaattcaatt gtttgccatg
    15061 cagcatggaa tgttatgggc aggaatgcca ttaatgccca caggcacaca acccgaagat
    15121 ttaaaccgta tgggcagtta tattggattg atggcacaat cggatagcgc gcctgttgac
    15181 gttactccgc ctgttggtga tttaagaaca gcagaatggt ttggtcagca tattgccaaa
    15241 actgtgcagc gaataataaa ttaggctatg attatcagag gctagctact aaaaagaggg
    15301 ataacacctc atgtatgagc aaccgcaaca tctaccgttc ctgcaagcta ttgcttggca
    15361 acattcggct gtgactgcgt taacagaaga tgaaatcttg tctctttatg aacgtggttg
    15421 gcattatcga ggggttttag cagatttaac aggtgaagag ttggcgtttg tgcgaaagtt
    15481 agcaactgaa aaaaaatcat ggattgttat tgatgtttgc ccttgaattt catcaaaaaa
    15541 tctcacaagt attgttagcc ttaaacgtag gcttttttca aaaagcctct gcctattttg
    15601 gtggtggtac cttacttgcc ttagcgtata acgaatatcg ttggagtaaa gatatagatt
    15661 ttgtttgtcc tgtcggtgaa cattatcggt atttacgcag caaaataatg gaagaaggtt
    15721 atcaagcgat ttttcaaaac gttcaaggca tacagttacc tagagaaatt aaagccgatc
    15781 aatatggtat tagatttcct gtggtgatag ataatacctt gataaagttt gagattattg
    15841 ctgaagcgcg tattctttta gagtcacccg tttatttaaa ttggttaaat atcccttgtt
    15901 tgagttttgt tgatcgttgt tgtgaaaaat tattggctaa tgcagatcgg tggaatgata
    15961 cggctgtaga gtcaagagat ttaatagatt tggctatgct aaggttagca tctcatattc
    16021 cacaattggc ttttgataaa gcggaagcgg cgtatgatgt aaaaaagcct ttgtttcggg
    16081 cgttaaataa ttttcaacaa aaaccagaat atcgtcaaaa atgttttgat gctttgcaca
    16141 tcagtaaaaa agcattaatt attgatggga tagatgcgtt agctttagat ttaggattat
    16201 tagctaccca gcgcacagag caagaaatgc atttagatga atacggctta ccaagagaca
    16261 tgacatgatt acgcatttaa ccattaccga ttttcaacca caagccacgg atattatttg
    16321 ggatgtacgt gatgccaaag cctatgccga aggccattgg cagccagcac aacatcaagc
    16381 gttagagagt attagtcttg ctaccttgca aacgctgcct agcgaacaaa cgatttatgt
    16441 gttgtgtggc ggtggtacaa aagctggaag agcagctcaa ttaatcgaag aattagataa
    16501 gcaacggcaa gtagtcattc ttactggtgg tacacgagca gcaaaagcgg ctggtttgcc
    16561 aatggttgaa ggagcttaat gctccctctc ccaatggaag aggggtgggg tgagattact
    16621 aaaaatcacg gttaatcatc acactgcccc aaattaaatc acgatgaccg tgaccttgtt
    16681 ttagttctgc actttgataa c
//
LOCUS       DAOQMB010000011        16554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1256_length_16554_cov_16.348991, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000011 DAOQMB010000000
VERSION     DAOQMB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 16554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16554
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1256_length_16554_cov_16.348991"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLY05_00975"
     CDS             complement(<1..1076)
                     /locus_tag="PLY05_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01903.1"
                     /translation="MSQIEQLFSRQFQQATEAFAKANLVNEWQQLQQQCLHQLQQLAL
                     SSDYAWRVLCQWPQVFWHAHQQGLLDNTLARPYFHQQTHQLFPLSQTDFMWMQSIRQY
                     RQQMMLRWIYRDVNQLSTLAELTDELSEFAEASVDAAIAYAEQALIARHGLPIGEDSG
                     QVQKMIVIGMGKLGAQELNLSSDIDLIFSYPEAGETNGRSVISNQEYFIKLAQQVIRL
                     LDQQTADGFVFRIDMRLRPWGDGSALALSFNAMERYYEQHGREWERYAFIKARVIAGD
                     ITSGQELLSSLRPFVYRRYIDYSAFAALRDMKAMIEREVRRKDMADNIKLGAGGIREV
                     EFIAQAFQLIRGGVFKDLQQRPLLP"
     gene            complement(1137..1706)
                     /locus_tag="PLY05_00980"
     CDS             complement(1137..1706)
                     /locus_tag="PLY05_00980"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC01904.1"
                     /translation="MLLYFKEIDSLRGEGKMRLVVAISLLLTNVAQAEIYKTYDKNGN
                     VIFSDVPSADAQHIEAKPIATVPALPRNVIDQKLKPQKDDKKLLPTSYNMTINGLASE
                     AVLQKEQETFNLGIQFEPALAKEHTLQVFLDGQDMGKNNLSPQINPRSLERGQHRLEF
                     KVLDAKQSVMQTQAIDFFIQQPSTKTPKK"
     gene            complement(1767..3176)
                     /gene="glnA"
                     /locus_tag="PLY05_00985"
     CDS             complement(1767..3176)
                     /gene="glnA"
                     /locus_tag="PLY05_00985"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HNC01905.1"
                     /translation="MANKALELIKENQAKWVDLRFTDIRGKEQHVSFPAYTVDEETFE
                     KGKMFDGSSIAGWKGIEASDMILMPDAETAFMDPFFDEPTVVISCDIIEPSTMQGYER
                     DPRSIARRAEAYLKATGIADTAYFGPEPEFFVFDEVKWDIDMSGARHTIYAEEAAWST
                     NKDYEGGNTGHRPRVKGGYFPVPPIDSAQDLRVAMCNAIESMIGEGKIEVQHHEVASC
                     QSEIGVAFNTLVRKADEVQVLKYAVHNVAHAFGKTATFMPKPLVGDNGSGMHVHQSLS
                     KDGKNLFSGDQYGGLSELALYYIGGIIKHARALNAITNPLTNSYKRLVPHYEAPIKLA
                     YSARNRSASIRIPYVSSPKAVRIETRFPDPGANPYLCFAALMMAGLDGIQNKIHPGDA
                     ADKNLYDLPPEEDALIPTVAESLEQALDCLKADHEFLLKGGVFTKEFLDAFIELKTEE
                     VRRLNTTVHPIEFDMYYSV"
     gene            complement(3972..>4696)
                     /locus_tag="PLY05_00990"
     CDS             complement(3972..>4696)
                     /locus_tag="PLY05_00990"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017388396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNC01906.1"
                     /translation="SIRIGVNAGSLEKDLQKKYGEPTGEALLESAMRHIDILDRLDFQ
                     EFKVSVKASNVFLTLDAYRLLSAQIEQPLHLGVTEAGVFRSGTVKSAIALGGLLLDGI
                     GDTIRISLAAEPEDEVKVGFDILKALGIRSNGINFIACPSCSRQEFDVIRVMNALEAR
                     LEDVRIPMDVSVIGCKVNGPGEAKEADIGVVGAAPKSLVYRNGEKSHLIPTDNLVDEI
                     EAMVRAKVAAEQEKQAKIIVKG"
     assembly_gap    4697..4796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4873..5661)
                     /locus_tag="PLY05_00995"
     CDS             complement(4873..5661)
                     /locus_tag="PLY05_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNC01907.1"
                     /translation="MQQASSSIQAAHDLSLGQRLKAYREQKGLSVEQVAQQLKILPRM
                     VEDIEAENYQALPEPVFVKGYLRLYADLLSVPSQEICHLFDAAYTKHHTEAHKTTPQI
                     LQVLETNTHAKPVRYHLRQNHKWLTIAIKVLLLVFIIGAIVAGLWQSTLFRQNQVGNS
                     VESSTHIENRTIALPNVTTPRQSVDTLNLRFSAQTKLRIADANGQELVNTTKQAGETL
                     SLQGQSPFAIELNPATAVEFRFNNNVIDLKPYTVNGMVNFRLSR"
     gene            complement(5682..6452)
                     /gene="pilW"
                     /locus_tag="PLY05_01000"
     CDS             complement(5682..6452)
                     /gene="pilW"
                     /locus_tag="PLY05_01000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HNC01908.1"
                     /translation="MIKFSFTLMMIALLSACAVQEFEGERKANPTAAARARVAIAAEY
                     IQKNQLELAQQHLQRAIEQDPKLPEAYNVMGVLLEKDDNFKESEQNYRKALSLKAEYP
                     QARHNYGVLLHRQKRYKEALAQFEMAANDLSYERRDVSLEYVANIALQMNDIVKAKQH
                     YQRLLKINPSLTTPALVLAQFAYDDKQFDEAERFYQRYLRNIGKESQSAQGLWLGIRL
                     AKLKNDHNAMANYEFFLKKLYPQSPEYQEYIKQFGTGT"
     gene            complement(6449..>7587)
                     /gene="rlmN"
                     /locus_tag="PLY05_01005"
     CDS             complement(6449..>7587)
                     /gene="rlmN"
                     /locus_tag="PLY05_01005"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNC01909.1"
                     /translation="KINLLGMTRSQMEEFFVSIGEKKFRAAQVIKWIHQLGVDNFDEM
                     SNVSKALRAKLAEVAEIRPPKVVYKEFSNDGTRKWVLDVGNNSMVETVLIPADGDRKT
                     LCVSSQVGCALDCSFCSTGKQGFQRDLSLAEIIGQVWVANRSYFEENQDYDSRERAIT
                     NVVMMGMGEPLLNFDAVVPAMELMLDDYAYGLSKRRVTLSTSGIVPMIDKLSEVIDVS
                     LAVSLHAPNDELRNELVPINKKYPLAELTAACRRFLAKFQGEEGGRRKITMEYVMLAG
                     VNDQPEHAKQLIKLLKDVPSKINLIPFNPFPHAPYGCTPRQEILAFQRTLTEAGFICT
                     IRTTRGDDIDAACGQLVGKVQDRTRRAERWQQKTRAGEIIRTQS"
     assembly_gap    7588..7687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7790..8221)
                     /gene="ndk"
                     /locus_tag="PLY05_01010"
     CDS             complement(7790..8221)
                     /gene="ndk"
                     /locus_tag="PLY05_01010"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNC01910.1"
                     /translation="MAIERTLSIIKPDAVAKNVIGQIYSRFEGAGLQIVAAKMKHLSK
                     AEAEGFYAEHKERGFFADLVAFMTSGPVIIQVLEGEGAVLKHRDIMGATNPKNAAAGT
                     IRADFAASIDENAVHGSDSETSAAREIAYFFSAIEVAPRTR"
     gene            complement(8398..9240)
                     /gene="rsmI"
                     /locus_tag="PLY05_01015"
     CDS             complement(8398..9240)
                     /gene="rsmI"
                     /locus_tag="PLY05_01015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNC01911.1"
                     /translation="MTGLLYVVATPIGNLEDISARAIKVLSQVNRIAAEDTRHSSRLL
                     QHLGINTPLVAVHDHNEAGRVQSLIDQLLAGDSIALISDAGTPLISDPGYRLVAAAHQ
                     ANIQVVPVVGACAAIAALSAAGLPSDRFSFEGFLAAKTSQRQTQLKALANESRTLIFY
                     EAPHRILECVQDMAAIFGADRRVVLARELTKTFETIKQCTLAELCVFVESDTNQQRGE
                     IVLVVDGAPEDSGLMAEKELDALLLKLLKYLPVKACAQLAAELTGHKKNALYDRALEL
                     QQMK"
     gene            9257..10291
                     /locus_tag="PLY05_01020"
     CDS             9257..10291
                     /locus_tag="PLY05_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HNC01912.1"
                     /translation="MSWIRFLSSSLLCLAGSVMASETSILLPSTGATAKTAEFVRNGL
                     LSGYYQSLAQNNHSPFLRFYDTTSSSTIETIIQQSQNNGSQLIIGPLFKEQVAQLVKN
                     PPNIPVLALNRVNNEGLSTLWQFALAPEEEIVPLIKVMQQNGIKKVRVIGLNDANSER
                     LRQAFEQTWTGMGGNLASNYTLTQTATDGFTQSIKKLITEPHNKDVQAFYLASPQSAL
                     YIMPLLNFYQHNPPPVYSNSLAYDSQKTSLERQDLNGLSFCGLPWVIEATKWSKPDDE
                     AINDDRLFAFGADAWLLSQHINSSKKIEFNARTGFISVEQGLIYRQPLCAKVSNGNAQ
                     ALNPTTGTTR"
     gene            10251..10625
                     /locus_tag="PLY05_01025"
     CDS             10251..10625
                     /locus_tag="PLY05_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNC01913.1"
                     /translation="MPKPSTQQLGQLAEQHACLYLQAQGLSLISQNFYSKYGEIDLVM
                     LDKQQTLVFVEVRYRSSNRFGGAVASVTLSKQRKIIAAARYFLYCYPQYASFNIRFDV
                     VAIDKQRINNITWLPAAFLVDD"
     gene            10652..11203
                     /locus_tag="PLY05_01030"
     CDS             10652..11203
                     /locus_tag="PLY05_01030"
                     /inference="COORDINATES: protein motif:HMM:NF016836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HNC01914.1"
                     /translation="MISSLWLTGCAQMIAATSGSEPVGKQEGERTLSQRIEDSSIENT
                     ANINLYKIDPEFKNANVVFMSFHSNVLITGQVTSIKHKQQVEDMVRSIREVNLVHNEL
                     VVTPQISSYLNRAKDGVLKLQIASKLTFMKEFPSSQCKVLVENGTVYLMAKLTRSQTE
                     QAINTIKETPDITRIVKLVDYLD"
     gene            complement(11235..11696)
                     /locus_tag="PLY05_01035"
     CDS             complement(11235..11696)
                     /locus_tag="PLY05_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HNC01915.1"
                     /translation="MTPSRPYLVRAIYEWLVDNNATPYLLVDATQANVRVPTQHVKDG
                     KIVLNIAPHAVKDLFIRNEGISFSARFGGVPMMVSAPMAAVAAIYARENGQGMFFDNA
                     DEYANVEEVESVAVAEPVKPNLSVATENTDSEVSSENPERPKGRPTLSVVK"
     gene            complement(11710..12312)
                     /locus_tag="PLY05_01040"
     CDS             complement(11710..12312)
                     /locus_tag="PLY05_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HNC01916.1"
                     /translation="MTLYSDANDHYSHRIRFVLAEKECAVEVVSINPHQKPEDLAHLN
                     PYNTLPTLVDRDVCLYETKIMIEYLDERFPHPPLLASYPVARALTRQYAYRIEKDWCR
                     LVDIILHEDDDEVVLNARKELRESLISIAPIFAEKPFFMSDEFTMVDVLLAPILWRLP
                     ILRIELPEKPCRPLLRYMERLFSREGFVNSLTPAEKHIRH"
     gene            complement(12448..13113)
                     /locus_tag="PLY05_01045"
     CDS             complement(12448..13113)
                     /locus_tag="PLY05_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HNC01917.1"
                     /translation="MKKLFLLFALMFSPVFALASGGGGCGTVHCEKAPVDLHNKESLQ
                     NGAKIFISYCLGCHTAKYLRYERMAQDLEIDPKLVEQYMMFTTDKIGDGMDTKVNKKD
                     QAKWFGNAPPDLSLETRFQSPDWVYTYLLNFYPDDKRPWGVNNRVFKDVAMPHVLDSL
                     EQDLGPEGFEEAVGDLVNFMTYMAEPIRTERERLGIWVLLFLAIMFVPVYLLNKEYWK
                     DVK"
     gene            complement(13113..14321)
                     /locus_tag="PLY05_01050"
     CDS             complement(13113..14321)
                     /locus_tag="PLY05_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HNC01918.1"
                     /translation="MSWVDARFPATETYEYHMSKYYAPKNFNFWYFFGVLSMVVLVNQ
                     LLTGFWLTMMYSPTADGAFDSIEYIMRDVEYGWLIRYMHSTGASAFFVVVYLHMFRGL
                     LYGSYQKPRELVWLIGMGIYLCLMAEGFFGYLLPWGNMSYWGAQVILNLVGAVPVVGD
                     ALVTWVRGDFLISGATLNRFFAIHVAVIPLILVGLVFIHLVALHHVGSNNPDGVDIKK
                     LKDENGVPLDGIAFHPYYTVHDLVGIVVFLFIFSLVIFFFPDGGGYFLERPNFEPSNP
                     LKTPPHIAPVWYYTPFYAILRAVPSKLGGVIAMGGAIAILFVLPWLDKSPVKSIRYKG
                     WMSKVWLAIFVVSFVGLGYLGAVPADPIKTVISQVLTILYFLFFLLMPIYTSIETCKM
                     PPKRVTGGKH"
     gene            complement(14345..14926)
                     /gene="petA"
                     /locus_tag="PLY05_01055"
     CDS             complement(14345..14926)
                     /gene="petA"
                     /locus_tag="PLY05_01055"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HNC01919.1"
                     /translation="MSTDGVNHSRRRMLIGTTAAVGAVGAAFVATPFVKSWNPSAKAK
                     AAGAPVTADLSKLELGQQMVVEWRGKPVWIVRRSPEALADLKKVAEDLRDPESNESVQ
                     PDFAKNEYRAIKPEYAILVGICTHLGCSPTYRPEVAPADLGQSWRGGFFCPCHGSTYD
                     MAGRVYKSVPAPLNLEVPPYKYLSDSVVLIGEA"
     gene            complement(15154..15546)
                     /gene="rpsI"
                     /locus_tag="PLY05_01060"
     CDS             complement(15154..15546)
                     /gene="rpsI"
                     /locus_tag="PLY05_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020292946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNC01920.1"
                     /translation="MTATTNYGTGRRKTATARVFLKAGAGAITVNGRALDVYFARETA
                     RMIVRQPLELIDGADKFDINVTVSGGGMGGQAGAIRHGITRALVEYDETLKSALRQAG
                     FVTRDSREVERKKLGLRKARKRPQYSKR"
     gene            complement(15557..15985)
                     /gene="rplM"
                     /locus_tag="PLY05_01065"
     CDS             complement(15557..15985)
                     /gene="rplM"
                     /locus_tag="PLY05_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNC01921.1"
                     /translation="MKTFSAKPETVKRDWYVVDASGKTLGRLASEIASRLRGKHKPEY
                     TPHVDTGDYIVVVNAGNLVVTGSKGQQKMYWRHTEFPGGIKGVNFDKLIAKKPELVLE
                     TAVKGMLPKGPLGYAMFSKLKVYGGAEHPHAAQQPVALDI"
     gene            complement(16240..>16554)
                     /locus_tag="PLY05_01070"
     CDS             complement(16240..>16554)
                     /locus_tag="PLY05_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNC01922.1"
                     /translation="VTIKQMLQQQTWAQIQQVAFNTMGKEALILALKDLAAQLGINLT
                     KRKALTIVPMIGAAIGAAVNASFIKDVGWAARRTFQEMWLLQHGGHIQQPLLLTNTAK
                     TR"
BASE COUNT         5047 a         3598 c         2992 g         4717 t
ORIGIN      
        1 ggcaataacg gtcgttgttg taaatcttta aacacgccgc cacgaattaa ttgaaaggct
       61 tgggcaataa actccacttc acgaatgcca cctgccccca atttaatatt gtcggccatg
      121 tctttacgcc gtacttcgcg ctcaatcatg gctttcatat cacgcagagc agcaaacgct
      181 gaataatcaa tataacgtcg ataaacaaaa ggacgtaaac tgctgagtaa ttcttggccc
      241 gatgtgatat cgcctgcaat tacccgcgct ttaataaaag cataacgctc ccattcgcgg
      301 ccatgttgct cgtaataacg ctccatggca ttaaatgata aagccaatgc gctgccatcg
      361 ccccaaggcc gtaaacgcat atcaattcta aagacaaaac catccgccgt ttgttggtct
      421 agcaaacgaa tcacttgttg tgccagttta ataaaatatt cttgattact aatcacactc
      481 cgaccattgg tttcgcctgc ttcgggataa ctaaaaatca aatcaatatc ggaggataaa
      541 ttaagctctt gtgcgcccaa tttacccata ccaataacaa tcattttttg cacttggcct
      601 gagtcttcac caatcggcaa accatgacgg gcaatcaagg cttgttcggc atacgcaatc
      661 gcagcatcaa cactggcttc ggcaaattct gataattcat cggttagctc tgctaaagtc
      721 gataattgat ttacgtcacg ataaatccag cgcaacatca tttgttgacg atattggcga
      781 atgctttgca tccacataaa atcagtctgg cttagaggaa ataattgatg cgtctgttga
      841 tgaaaatatg gccttgctag cgtgttatcc aataaacctt gttgatgagc gtgccaaaaa
      901 acttgtggcc actgacacaa cacacgccac gcataatcac tgcttaatgc caattgttgt
      961 aattgatgca agcattgttg ttgcagttgt tgccattcgt tgactagatt tgctttggca
     1021 aaagcctctg tggcttgttg aaactgtctt gagaatagtt gttcgatttg ggacatgatg
     1081 aattatcttg ttatcttaat ttacctcaaa gataacaaga tttacgttaa ttgccactac
     1141 ttctttgggg tttttgttga tggctgctga ataaaaaaat caatagcttg tgtttgcata
     1201 acactttgtt tagcatccag caccttaaac tctaaacgat gttgacctcg ctctaaagaa
     1261 cgtgggttta tttgaggaga taagttattt ttccccatat cttgtccatc taaaaatacc
     1321 tgcagagtgt gctctttcgc taatgctggt tcaaattgta tgcctaaatt aaaagtttcc
     1381 tgctcttttt gtaatacagc ctcagaagct aagccattta tagtcatatt ataggaggtt
     1441 ggtaataatt ttttatcatc tttttggggt ttaagctttt ggtcaatgac atttcgaggc
     1501 aacgctggga ctgtggcaat gggttttgct tctatatgtt gtgcatcagc actcggcacg
     1561 tcagaaaata tcacattgcc atttttatca taggttttat aaatttcggc ctgagcgaca
     1621 ttcgtcaata ataaacttat tgctaccact aaacgcattt tgccctctcc cctcaaagaa
     1681 tcaatttcct taaagtataa aagcaaaaag gctcattttc atgagccttt ttgtcaaagc
     1741 gtgacgcttt atgtatttac agtgtattac acgctgtagt acatatcgaa ctcaatagga
     1801 tgaactgttg tgttcaagcg acgaacttct tcggttttta actcaataaa agcgtcgagg
     1861 aattctttag tgaacacgcc accttttaac aagaattcat ggtcagcttt taagcagtct
     1921 aaagcttgtt ctaaactttc ggcaaccgtt gggattaatg catcttcttc tggtggtaag
     1981 tcatacaagt ttttgtctgc tgcatcgcct ggatggattt tgttttgaat accatctaaa
     2041 ccagccatca tcaaagcagc aaaacataag tatgggtttg cacctggatc tgggaagcga
     2101 gtttcgatac gaacagcttt tggactgctt acgtatggaa tacggataga agcagagcgg
     2161 ttacgtgcag agtaagctaa cttgatgggt gcttcgtagt gaggaaccaa acgcttgtag
     2221 gagttagtta atgggttggt aatcgcattc aacgcacgag cgtgtttgat gataccacca
     2281 atgtaatata aagctaattc agacaagcca ccgtattggt cgccagagaa taagttttta
     2341 ccatctttgc ttaacgattg gtgtacgtgc ataccagaac cgttgtcacc aactaatggt
     2401 tttggcataa aggttgctgt tttgccgaaa gcgtgagcaa cattgtgtac agcatatttt
     2461 aatacttgta cttcgtcagc tttacgtact aaggtgttaa atgcaacacc gatttcgctt
     2521 tggcaagaag ccacttcgtg gtgttgtact tcgattttgc cttcaccaat catcgactca
     2581 atcgcgttac acatagcaac acgtaaatct tgagcagagt caattggagg aactgggaag
     2641 taaccgcctt ttacacgtgg acggtgacca gtgttaccac cttcgtagtc tttgttggtt
     2701 gaccaagcag cttcttcggc ataaatagta tgacgagcac cgctcatgtc gatgtcccat
     2761 tttacttcgt caaatacgaa gaattctggc tctggaccaa aataagcagt atcagcaata
     2821 cctgttgctt ttaagtaagc ttcagcacga cgagcgatag aacgtgggtc acgctcataa
     2881 ccttgcatag tgcttggctc aatgatgtca caagaaataa caacagttgg ctcatcaaag
     2941 aatggatcca tgaatgcagt ttctgcatct ggcatcaaaa tcatgtcaga agcttcgatg
     3001 cctttccaac cagcaatcga agaaccgtca aacatttttc ctttttcaaa agtttcttca
     3061 tcaactgtgt aagctgggaa agaaacgtgt tgctctttac cacgaatatc ggtaaagcgt
     3121 aagtcaaccc atttagcttg attttcttta atgagttcta aggctttatt ggccatttat
     3181 agcagctccc aaaatagcaa attatagagg acttgcgcat ttcttcgaaa ttagcgcgtt
     3241 ttgcttacta aaaatcgtta aaaccgattt ttagtaagca aataagcgat aaccgcgtaa
     3301 gtcctaaaat agaggactaa acgtccaacg ttttagcaat aagtaattag ttatttaact
     3361 aatgttatcg acagtcaacg atgacaaaac gaggttttat agtactgtga acaatactca
     3421 aaaactggct cgaaaataat tcctaaaaag ctcgggcagt ttaacagaat cctagctagg
     3481 ctcaagtcac aagatgcgat ttacatacac actttaacgt tatacctagc tctacactct
     3541 acaactgcca agtttcggta tttaaaacag caatttttat gccatcaaaa aggtatattt
     3601 tggcgcactt ctgagctatt taatgcaaca tattgcacca aaaaataaca atcaccccaa
     3661 aaatgcacca aaataaagca aattagctta ctgttaattt ggttgagcca ttattgacca
     3721 ccactgtttt tgctctacct gacaatatca ctgaatcagc agtcgggtta ttgctcaaac
     3781 tggcaatatg atgatattta gcctctacgc gagcaggggt tacattgacg gtaataagga
     3841 ctagtctaga aattattaac ttgctgattg tttatgtaaa aaatgacaaa aagattgacg
     3901 tttgatatca aaaggttttt tttgtggatt aaatcgtgtg agtacctgca tccttgcaag
     3961 tgaagtgagt gttatccctt aacaataatc tttgcttgtt tttcttgctc agcagccact
     4021 ttggctctga ccatcgcttc gatttcatcg actaaattat ctgtcggaat caaatgactt
     4081 ttttcaccat tacgatacac caaactcttt ggcgcagcac ccaccacacc aatatcagcc
     4141 tctttagctt cacccggccc gttaacttta cagccaatca ctgacacatc catggggata
     4201 cgcacatctt ctaaacgggc ttctaaggcg ttcatgactc gaatgacatc aaattcttga
     4261 cgagagcaac tggggcaagc aataaaatta atgccattag aacgaatacc caaggctttt
     4321 aaaatatcaa aaccaacttt aacttcatct tcgggttcag ccgctaaaga aatacgaatt
     4381 gtgtcgccaa tcccatctaa taacaaacca cccaacgcaa tggccgattt cactgtaccc
     4441 gatctaaaca cccctgcttc ggtcacgcct aaatgcaaag gttgctcaat ttgagctgac
     4501 aataaacgat aggcatctaa ggttaaaaat acattagagg ctttaacact gaccttaaac
     4561 tcttgaaaat ctaaacggtc taaaatatca atatgacgca ttgccgactc aagcaatgct
     4621 tcgcctgttg gctctccata ttttttttgt aaatcttttt ctaaagagcc tgcattcacg
     4681 ccaatccgaa tcgacannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcat
     4801 caccaccgac atacacagaa ccaacacgaa ttttacgggt gacacgacgt ttaatgggcg
     4861 aaatgtgcat tcttacctcg ataagcgaaa gttaaccata ccattaaccg tatatggttt
     4921 taaatcaatg acattattat taaaacgaaa ctcgacagcc gttgcgggat ttaactcgat
     4981 ggcaaaaggt gattgacctt gaaggcttaa agtctcacct gcttgcttag tggtattgac
     5041 taactcttgg ccattggcat ctgcaatacg caacttggtt tgagcagaaa aacgtaaatt
     5101 gagtgtatcg acactttgcc gaggcgtggt gacattaggc aaggcaatcg tacgattttc
     5161 tatatgagtt gatgactcta cgctattgcc aacttgattt tgcctaaata acgtactttg
     5221 ccataaacca gcaacaatgg caccaataat aaatacgagc aataatactt taatcgctat
     5281 cgttaaccat ttatgatttt gacgtagatg ataacgaaca ggcttagcgt gtgtattcgt
     5341 ttctaacact tgcagtattt gaggtgttgt tttgtgtgct tcggtatgat gtttggtgta
     5401 tgccgcatca aataaatgac agatttcttg gcttggtaca gataataaat cggcatacaa
     5461 acgcaaataa cctttaacaa agactggctc tggcaaggct tgataatttt cggcctcaat
     5521 atcttctacc attcgtggca aaattttaag ctgttgtgca acttgctcca cactcaagcc
     5581 tttttgctca cgatacgctt taaggcgttg ccctaatgat aagtcatgtg ctgcttgtat
     5641 agaggaagaa gcttgttgca ttgtacactc ctgacttatc gttaagtacc tgtgccaaac
     5701 tgcttaatat attcctgata ttcagggctt tgtggatata atttttttaa aaagaactca
     5761 taatttgcca ttgcattatg atcatttttt aactttgcca aacgtattcc caaccacaag
     5821 ccttgtgcgc tttgactttc tttgcctata ttacgcaaat aacgctgata aaaacgttcc
     5881 gcttcatcaa attgtttatc atcatacgca aattgtgcta ggactaaggc tggcgtagtt
     5941 aacgacggat taatttttaa taatcgctga tagtgttgtt tggctttaac tatatcattc
     6001 atttgcaaag caatattagc aacatattcc aacgaaacat ctcgccgctc ataacttaaa
     6061 tcattcgccg ccatttcaaa ttgcgctaaa gcttctttat accgcttttg tctatgcaac
     6121 aaaaccccgt aattatgacg agcttgtgga tattctgctt ttaaacttaa tgccttacga
     6181 taattttgct cagactcttt aaaattatca tctttttcta gcagcacccc catcacatta
     6241 taggcttctg gcaacttagg gtcttgttca atggcgcgtt gtagatgctg ttgtgccagc
     6301 tctagttggt ttttttgaat atattccgca gcaatagcca cacgcgctct agcagccgct
     6361 gttggatttg ctttacgttc accttcaaac tcttggactg cacaagcaga taacagggca
     6421 atcatcatta atgtaaaact aaacttgatc atgattgtgt ccgaataatc tcacctgctc
     6481 gcgttttttg ttgccaacgc tctgcccgac gggtacggtc ttgaactttc cccactaatt
     6541 gtccacaagc cgcatcaata tcatcgccac gggtagtccg aatcgtacaa ataaaacccg
     6601 cttcggttaa tgtgcgttga aacgccaaaa tttcttgacg tggcgtgcaa ccataaggtg
     6661 catgagggaa tggattaaac ggaattaaat taattttact gggtacatct ttgagtaatt
     6721 taatcaattg tttggcgtgt tctggctgat cgttcacccc tgccaacatc acatattcca
     6781 tagtaatttt gcgacgccca ccttcttcac cttgaaattt agctaaaaag cggcgacagg
     6841 cagcggttaa ttctgccaat ggatattttt tattaatggg tactaattcg ttgcgtaact
     6901 catcatttgg tgcatgtaaa gacacagcta atgacacatc aatcacttct gataacttat
     6961 caatcattgg tacaatacca gacgttgata aagtaacacg acgtttagac aaaccatagg
     7021 cataatcgtc taacatcaac tccatcgctg gtacaacagc atcaaaattt aataaaggct
     7081 cacccatgcc catcatcacc acattggtaa tggcacgttc acggctatca taatcttgat
     7141 tttcttcaaa ataagagcgg ttggcgaccc acacttgacc aataatttcc gccaaactta
     7201 aatcgcgctg aaaaccttgt ttgcctgttg agcaaaaact acagtctaaa gcacagccaa
     7261 cttgtgatga cacacataag gttttgcgat caccatcagc aggaattaac acagtttcaa
     7321 ccatgctatt gttgccgaca tccaacaccc atttacgtgt accatcatta gaaaattcct
     7381 tataaaccac ttttggtggg cgaatctcgg caacttcggc taatttggca cgtaatgctt
     7441 ttgaaacatt actcatttca tcaaagttat ccacacccaa ttgatgaatc catttaatca
     7501 cttgtgctgc acgaaacttt ttttcaccaa tactgacaaa aaactcctcc atttgggagc
     7561 gtgtcatacc aagtaaattg attttttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnntag cacaaaaaca aaaggcgcaa ttacgcgcct tttgtatcta caccgaagac
     7741 ttatggctga tttttttaaa ccttgcttaa gtcttgttaa ttttaaaaat taacgtgtac
     7801 gtggtgcaac ttcaatagca ctgaagaaat aagcgatttc acgtgcagca gaagtttcag
     7861 agtcagaacc gtgaacagca ttttcgtcaa tagaagcagc aaagtcagca cggatagtac
     7921 cagcagcagc attttttggg ttggttgcgc ccataatgtc acgatgcttt aatactgcgc
     7981 cttcgccttc taatacttga atgatcacag gaccagaagt cataaaagcg actaaatcag
     8041 caaagaaacc acgttctttg tgttcagcat aaaaaccttc agcttcggct ttagataaat
     8101 gcttcatttt agcagcaacg atttgtaaac cagcaccttc aaaacggctg taaatttgac
     8161 caataacgtt tttagcaacg gcatcaggct taatgataga taaagtacgt tcgatagcca
     8221 tgtttagctc cttcaataga aataaagttt aacgacaaat tggggcgcga ttatacgcag
     8281 attttagcaa tttgtcgcgc taggatttta ataactaagt tcaatgggta ctaaacctgt
     8341 gtgggtatgt attaatgtgc ctgcgacaaa accttgttca cgattcatta tttttcctta
     8401 tttcatttgc tgcaattcca acgctcgatc atacaaggca tttttcttat gacctgttaa
     8461 ctcggcggct aattgcgcac acgctttaac aggtaaatac tttaataact ttaacaataa
     8521 cgcatctaac tctttttctg ccattaaacc tgaatcttct ggtgcaccat caaccaccaa
     8581 cacaatttcg ccgcgttgct gatttgtgtc cgactccaca aacacacaca actctgccaa
     8641 agtacattgt ttaatggttt caaaggtttt ggttaactct ctagccaaca ccacacgcct
     8701 atctgcccca aaaatcgccg ccatatcttg cacacactcc aaaatacgat gtggtgcttc
     8761 ataaaaaatc aacgttctgc tttcattggc taaagctttt aattgggttt gacgttgact
     8821 ggttttagcc gccaaaaacc cttcaaaact aaaacgatca ctgggtaaac ctgctgccga
     8881 taaggcggca atcgctgcac acgctcccac cacaggcacc acttgaatat tggcttgatg
     8941 tgctgccgcc accaaacgat aacccggatc actaattaac ggtgtacctg catcggaaat
     9001 taaggcaata ctatcgcctg caagcaactg atcaattaag ctttgcacac gccctgcttc
     9061 gttgtgatca tgtacggcaa ctaatggtgt attaatgcct aaatgctgta acaaacggct
     9121 agaatgtcgt gtatcctccg ctgcaatacg attaacttgg cttaacacct taatcgcccg
     9181 tgcgctaata tcctctaggt tgccaattgg ggtggcaacc acgtaaagta accctgtcat
     9241 atttaggaac tctgttatgt cgtggattcg ttttttgagt agtagtctgt tgtgcttggc
     9301 tggttctgtg atggctagtg aaaccagtat tttactacct agtaccggtg caactgcaaa
     9361 aactgctgaa tttgtgcgaa atggtttgct gtcgggatac tatcaaagtt tggctcaaaa
     9421 taaccacagc ccgtttttac gcttttatga tacaacgagc agctctacaa tagaaaccat
     9481 tatccaacaa agccaaaata atggtagtca actgattatt ggccctttgt ttaaagagca
     9541 agtcgcacaa ttagttaaaa atcctcccaa catccccgta ttagctttaa accgagttaa
     9601 taacgaaggt ttatccacac tttggcaatt tgcccttgca cctgaagaag aaattgttcc
     9661 actgattaaa gtgatgcagc aaaacggcat caaaaaagtg cgagttattg ggttaaatga
     9721 tgctaatagc gaacgcttgc gccaagcgtt tgagcaaaca tggacaggca tgggcggcaa
     9781 tttagcaagc aattacacac tcactcaaac cgcaaccgat ggttttaccc aaagcattaa
     9841 aaaactgatt accgaaccac acaacaaaga tgtacaagct ttttatttag caagcccaca
     9901 atctgcttta tacattatgc cgctattgaa tttttaccaa cataatccac cgcctgttta
     9961 tagcaactct ttagcttatg acagtcaaaa aaccagttta gagcgtcaag acttaaatgg
    10021 tttaagtttt tgtggtttac cgtgggtgat tgaagcaaca aaatggtcaa aacctgatga
    10081 cgaagctatt aatgacgacc gtttatttgc ttttggtgca gatgcgtggt tattaagtca
    10141 acatattaac agttccaaaa aaatagaatt taacgcccga acaggcttta ttagtgttga
    10201 acaaggctta atttatcgtc aacctttatg tgctaaagtg agcaatggaa atgcccaagc
    10261 cctcaaccca acaactggga caactcgctg aacaacatgc ctgtctgtat ttgcaggcac
    10321 aaggtttatc gctgattagc caaaactttt atagtaaata tggcgaaatt gatttggtga
    10381 tgcttgataa acagcaaacc ttagtgtttg ttgaagttcg ctatcgtagc tctaatcgtt
    10441 ttggcggcgc agtagcctcc gttacgttaa gcaaacaacg aaagattatt gcggcagcgc
    10501 gttatttttt atactgttat ccacaatatg ccagttttaa cattcgtttt gatgttgtgg
    10561 ctattgataa acaaaggatt aacaacataa catggctacc tgccgctttt ttagtagacg
    10621 attagacatg aaaaaatctt tgatattttt aatgattagc agtttatggc tgacaggttg
    10681 cgcccaaatg attgccgcca cgtctggctc tgaacctgtt ggtaaacaag aaggtgagcg
    10741 tacgctgagc caacgcattg aagatagcag cattgaaaat actgcaaata ttaatttata
    10801 taaaattgat cctgagttta aaaacgccaa tgttgttttt atgagctttc atagtaatgt
    10861 actaattact ggtcaagtga cgagcatcaa acataaacaa caagttgaag acatggtacg
    10921 cagtattaga gaagtgaatt tggtacacaa cgaattggtt gtcacaccac aaattagctc
    10981 ttatttaaat cgtgctaaag atggtgtatt aaaactacaa atcgccagta agctaacatt
    11041 tatgaaagag tttccgagta gtcaatgcaa agtattggtg gagaatggaa ctgtttattt
    11101 gatggcaaaa ctgactcgct ctcaaaccga acaggcaatt aataccatta aagaaacacc
    11161 cgatattacg cgaattgtta aattggttga ttatttggat taaaaaatcc ctctcgtgta
    11221 gagagggtat gtacttattt caccaccgat aatgttggcc taccctttgg tcgttcagga
    11281 ttttctgatg atacttcact gtcagtattc tcagtcgcta cgctcaagtt aggtttaaca
    11341 ggctcagcaa cagcaacgct ctctacttct tcaacattgg catattcatc agcgttatca
    11401 aaaaacatac cttgtccatt ttcacgcgca taaatcgctg caaccgccgc cattggtgca
    11461 gacaccatca ttggcactcc gccaaaacgt gcagaaaagc taataccttc attacgaata
    11521 aataaatctt tgaccgcatg aggcgcaata tttaacacaa ttttgccatc tttaacgtgt
    11581 tgtgttggca cacgaacatt agcctgtgtg gcatccacta acaaataagg ggtagcatta
    11641 ttatcaacta accactcata aatggctctg accaaatagg gacgacttgg agtcataatt
    11701 atcttctcat tagtgccgta tatgtttttc ggctggtgtt aaactattca caaaaccttc
    11761 tcggctaaat aaacgctcca tataacgcaa caagggacga caaggctttt cgggtaactc
    11821 tattcttaat attggcagtc gccataaaat cggggctaaa agtacgtcca ccatggtgaa
    11881 ctcatcactc ataaaaaatg gcttttcggc aaaaattggc gcaatactaa ttaaactttc
    11941 acgcaattct ttacgcgcat ttaataccac ttcatcatca tcttcatgca aaataatgtc
    12001 aactaagcga caccaatctt tttcaattcg ataagcatat tgacgggtta aagctcgagc
    12061 aacaggatac gaagctaata atggcggatg aggaaaacgt tcatccaaat actcaatcat
    12121 aatttttgtt tcatacaaac aaacatcacg atcaaccaaa gtgggcaagg tattataagg
    12181 atttaaatgc gccaaatctt caggcttttg atgagggtta atactcacaa cttctactgc
    12241 acactccttt tcggccaaaa caaagcgaat tcgatgacta taatggtcat ttgcatctga
    12301 gtataaagtc attactacag atgctcgctt attgattacc cccatacatt ttgctccttt
    12361 taaaattata tttaccttaa gtataacaat aacaaactct caaaaaaaag ggcaacttat
    12421 cgttgccctt tttgatggat ttaagtctta tttgacatct ttccaatact ctttgttcag
    12481 taaatacact ggaacaaaca taatggctaa gaataataat acccaaatac ctaaacgctc
    12541 acgctcagtg cggattggct cggccatata agtcataaag ttaaccaagt cgcctacagc
    12601 ttcttcaaaa ccttctggac ctaaatcttg ctctaagcta tccaacacat gaggcatcgc
    12661 cacgtcttta aagacacggt tattaacccc ccacggacgc ttatcatctg gataaaagtt
    12721 taacaaataa gtgtaaaccc aatctggact ctgaaaacgt gtttctaagc ttaaatcagg
    12781 tggcgcatta ccaaaccatt tagcttgatc ctttttattg accttagtat ccatgccatc
    12841 accaatttta tcggtagtaa acatcatata ctgttcaact aattttggat caatttccaa
    12901 gtcttgtgcc atacgctcat aacgtaagta tttagcagta tgacaaccta aacagtagct
    12961 aataaaaatt ttagcaccat tttgtaaaga ctctttgttg tgcaaatcaa cgggggcttt
    13021 ttcgcagtgt actgtaccac aaccaccgcc accacttgct aaagcaaaaa caggactaaa
    13081 cattaaagcg aataataaaa atagcttttt cattagtgct ttcctccagt cacgcgtttt
    13141 ggtggcattt tacatgtctc aattgatgta taaattggca tcaataagaa gaacaagaaa
    13201 tataaaatag tcaacacttg agaaataacc gttttgatag gatcagcagg aacagcacct
    13261 aaataaccca aaccaacaaa actcaccaca aaaatcgcca accaaacttt actcatccag
    13321 cctttataac gaatagattt gacaggactc ttatctaacc acggtaaaac aaagagaatc
    13381 gcaattgcac cgcccatcgc aatcacaccg cctaacttag agggaacagc acgtaaaatg
    13441 gcataaaatg gcgtgtaata ccaaactggt gcaatatgtg gtggtgtttt taaaggatta
    13501 gaaggttcaa agtttggtct ctctaagaag taaccaccgc catcagggaa gaagaaaatc
    13561 accaaagaga aaataaataa aaataccaca ataccgacta aatcatgaac ggtgtaatag
    13621 ggatggaacg caataccgtc taatggcacg ccgttttcat ctttcaattt tttgatatca
    13681 acaccatcag ggttattaga accaacatga tgcaatgcga ctaaatgaat aaaaaccaaa
    13741 cccactaaaa tcaatggaat aaccgcaacg tgaatcgcaa aaaaacggtt taaggttgca
    13801 ccagagatca agaagtcacc acgaacccaa gtcactaaag catcaccgac aacaggtaca
    13861 gcacccacta agttaagaat aacttgtgcg ccccaatagg acatattgcc ccaaggtaat
    13921 aaataaccga aaaaaccttc agccattaaa cacaagtaga tacccatgcc aattaaccac
    13981 actaattcac gtggtttttg ataagaaccg tagagcaaac cacggaacat gtgcaaatac
    14041 acaacaacaa agaaggcaga agcacccgta gagtgcatat aacgaattaa ccaaccatat
    14101 tccacatcac gcataatgta ttcgatagag tcgaatgcac catctgcggt tgggctatac
    14161 atcatggtta accaaaaacc agtcaatagc tggtttacca aaaccaccat cgacaacaca
    14221 ccaaaaaaat accaaaagtt aaaattcttg ggtgcatagt atttagacat gtgatattca
    14281 taagtttcag tcgctggaaa acgcgcatca acccatgaca tcaaattaga cattaactta
    14341 ttcattatgc ctccccaatg aggacaacac tgtcactcaa atatttatag ggtggaactt
    14401 ctaagtttaa gggtgcaggt acactcttat aaacacgccc tgccatatca taagtagaac
    14461 catgacatgg acagaagaaa ccaccacgcc agctttgacc taaatcggca ggcgcaactt
    14521 ctggacgata cgttggtgaa caacccaaat gggtacaaat accgaccaaa atcgcgtatt
    14581 ctggcttaat cgcacgatat tcatttttcg caaaatctgg ttgtacagac tcattggact
    14641 ctgggtcgcg taaatcctct gcgactttct ttaaatcagc caaggcttct ggtgaacgac
    14701 ggacaatcca tacaggctta ccacgccatt caaccaccat ctgctggcct agttctaatt
    14761 tactcaaatc tgcggttacc ggcgcaccag cagcttttgc ttttgcactc ggattccacg
    14821 acttcacaaa cggagtggct acaaaggctg cccctactgc accaacggca gcagtggtac
    14881 caattagcat ccgtcggcga ctatgattta cgccgtcagt actcattcac gtactcccca
    14941 aaacaagcat aaataggccg tgcagcacac aactgcaaag cccaccaaaa aacttggcaa
    15001 gtctaaataa tttaacgtat tgttacaaga aaaaaccaca aaatcacaaa ggatttcgtg
    15061 taattaatat tattctacta atttttacag ccaataaaaa aaacaatccc cagacaaagc
    15121 tggggattgt ttgcaaacat aatcgaaaat cgattaacgc ttggagtatt gtggacgctt
    15181 gcgagctttg cgtaaaccca attttttacg ttcaacttca cgagagtcac gagtcacaaa
    15241 gccagcttga cgtaaagctg attttaaagt ctcgtcatat tctactaaag cacgagtaat
    15301 gccgtgacga atagcaccag cctgaccacc cataccgcca ccacttacag taacgttgat
    15361 gtcaaacttg tctgcgccat cgattaattc taaaggctga cgtacgatca tacgagctgt
    15421 ttcacgggca aaatacacgt ctaaggcacg accgttaacc gtgatagcac cagcaccagc
    15481 ttttaagaac acacgggcgg tagcagtttt acgacggcct gtaccgtagt ttgttgttgc
    15541 tgtcataatt cacccattag atgtctaaag ctacaggttg ttgggcagca tgaggatgct
    15601 ctgcaccacc atacactttt aatttgctga acattgcata acccaaagga cctttaggta
    15661 acatgccttt aacagcggtt tctaacacga gttcaggctt tttagcgatt aacttgtcaa
    15721 agtttacgcc tttgatacca cctgggaact ctgtgtgacg ccaatacatt ttttgttggc
    15781 ctttgctacc agtaacaacc aagttacctg cattaacaac aacaatataa tcccctgtgt
    15841 caacgtgtgg ggtgtattct ggtttatgtt tgccacgtag acggctagcg atttcggaag
    15901 ctaaacgacc gagagtcttg ccagatgcgt caacgacata ccagtcacgc ttaaccgttt
    15961 ccggcttcgc actgaacgtt ttcattgcta caacgccttt atcaaattta catactagga
    16021 caggacttag gcaaaacaag ggagccagtg ggaggtagcc gcgtaagccc taacaattaa
    16081 actttgaatt tatgtacaac ataaactctt aggatgacat aaatggctct ttgcatcaga
    16141 gctgctaatt gctcttttta agccaattaa gccaacaata aaaatagaag gcggatatta
    16201 ctcaaggaac agttttttta caacaaaaat ctttaaaact taccgtgttt tagcggtatt
    16261 tgtgagtaat aacggttgtt gaatgtgtcc accatgttgt aataaccaca tttcttgaaa
    16321 agtgcgcctt gcagcccaac ccacatcttt aataaaactg gcattgaccg ctgcaccaat
    16381 ggccgcgcca atcattggca caatagtcaa tgctttacgt ttagtaagat taatacctaa
    16441 ttgtgcggcc aagtctttta acgctaagat taatgcttct ttgcccatgg tattaaaagc
    16501 cacttgctga atttgcgccc atgtttgttg ttgcaacatt tgtttaatgg tcac
//
LOCUS       DAOQMB010000012        15674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1361_length_15674_cov_23.042512, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000012 DAOQMB010000000
VERSION     DAOQMB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 15674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15674
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1361_length_15674_cov_23.042512"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..2697)
                     /locus_tag="PLY05_01075"
     CDS             complement(154..2697)
                     /locus_tag="PLY05_01075"
                     /inference="COORDINATES: protein motif:HMM:NF040662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HNC01923.1"
                     /translation="MTVKISIYDHVFKAIEPEIVEYSSVAEWILDNKKRLVNFAVFNG
                     QPSNETDITKNVNALMNNTGEYVVLITPSAPVVAFVVAHWVAISVVVTVLTAGYTLYQ
                     LSNLKTPSNLNRTQQSPNNALAGRTNEPRVLQRIEDIYGQVRAYPSLLQPVYSKYIDN
                     VQYEYSYMCIGRGWYAVDDVRDGETPLNEITGTKAEFFNPFTSPNSGSPFLTIGGSIS
                     EPVLTVKKSNNVDGAVLQARNQFSLAETTGYLDFYKASTIPSSYDRIGGISNELYDNI
                     SIGDVLTISGSIGGYNGVYTVRNKLGGTNILELTTATFSATATTNATITVTTGNPEWT
                     DWVTLTDTDMTQIWVNLIAQQGLYYQDSEGSYNLTVNYEIQYQKVVSGTPTGSIYTNS
                     GSMSGQSPSQVAKTIEITTGWLGATRVRVRRSNDHDFGFAGNVVDEIKYESLSAVTLI
                     TNSDFGNVTTVQVITKATQRALSLKERKLNCLVTRKLPTYNGTTWSGVFDTDGSVLSG
                     TIAATTSFVNILAAITQDPFIGRQSLTTIDIAQIYALYSQIGGYFGLGTEPMAFHYTL
                     DSDNISYEETIRMIANAVFCIAYRQNGKIRFSFDKPQTASTALFTHRNKKPASDVISR
                     RFAADDEYDGIDLTYNDDVSDAQETIKIPTDGSATNYKKIDLVGVRFFDVAYIRAMRE
                     YNKLKYQRISIETETTTDGRLLLPNQRIDIVDNTRFDSQDGEVIAQSGLTLTLSRDVV
                     FGVGTHSILLMKRDGSLESISCTAGTQANQVVLDNAPSEAINTTHGGANGVRTIFSFA
                     ADSAESANSYLVQEVNISDKSYVKVSAINYDANYYSDDLETIPNREDVL"
     gene            complement(2681..3049)
                     /locus_tag="PLY05_01080"
     CDS             complement(2681..3049)
                     /locus_tag="PLY05_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HNC01924.1"
                     /translation="MIDKYIAKHYEWPPCWQLVADVYVNELGLSLDDYTPKSNTMRDV
                     ANAFRIALHNNKHGFAKTEKANNLDVVLLGKNKKVTHCGLFYNQGVLHSLRDMVIWQP
                     LTQIADVYGLIEYYRYDCKN"
     gene            complement(3046..3555)
                     /locus_tag="PLY05_01085"
     CDS             complement(3046..3555)
                     /locus_tag="PLY05_01085"
                     /inference="COORDINATES: protein motif:HMM:NF020449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HNC01925.1"
                     /translation="MSLDIEARLREFLASAPQTIYMIEVISIAHSDLTQTYHLWREPS
                     NGGVVDENDNVLTVRSTNFTVALAGTPDNLDQKFTVNIDTTDPDNLLRKELDRIPLDT
                     TEKILLTYRAYLSDDLTEPQAVQTLQVESIAYNRGTATISAIAPKLNVNRTGELYTFS
                     RFPMLRGFL"
     gene            complement(3552..4052)
                     /locus_tag="PLY05_01090"
     CDS             complement(3552..4052)
                     /locus_tag="PLY05_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01926.1"
                     /translation="MPTPVLPRDLYPVTSPSGYSYASAQGVHMTQVEGGFNRYAMDFD
                     RGTRVYNVALACTAGHLLVWELFYLRIIKKGALAFEMPLDSGTGLEQHLVNIIPNSVN
                     TTETDGNNFVVTFQVEAESKIYDFTDGGAEFILSYWEDYGDLGGLGDFFDRLSEFVLS
                     DTLVLV"
     gene            complement(4056..>4355)
                     /locus_tag="PLY05_01095"
     CDS             complement(4056..>4355)
                     /locus_tag="PLY05_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01927.1"
                     /translation="RAMGGDVQAGNSYLVGERGAEIITMGGNGHVTPNHKLGGDTKVT
                     IVNQTTGKIDKVEERRMPDGELILTVIEAVAAQTLDPNSKISRNQAAAFGLQRRR"
     assembly_gap    4356..4455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4456..6541)
                     /locus_tag="PLY05_01100"
     CDS             complement(<4456..6541)
                     /locus_tag="PLY05_01100"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HNC01928.1"
                     /translation="MADLLMIGLGVDTRRLRDGERALNNLQKTGNKTEAALGRMAATL
                     ASAFAVHKIIEYADSYTQLQNKLKLVTDSTEALAYATDDVYQIASRTNQALGATGDLY
                     FKISQNADKLGLSVSDVSRVTETFAKTLAISGAGTQQAEAAILQFSQALASGVIRGDE
                     FNSVAENAPAAMDAFSRALGVSKGELRKLAAEGMLTTDILISALQEQSSVVDELASKT
                     SATIGQASTKLKNAMIVFVGQLDVATGASNSLVSSLEGITAWIDSGQPLETLTYQFTL
                     WSYAVDDVSSVTVDLLKYLGVLRKDGGDTASFLSDAFTKLPVNIATSFKIAQVEVKSF
                     FDYVKEGLIISAERWNELDKQRKDSIAAILAERDARLGAGNTAVKAIQDERKAREELR
                     KQKELDALLDDWINESTRKSTKELFEERKKQLQAEKEAEKNKKQQSKDADQLQEKYKS
                     LLLSAKERVELWGNESELAKLNFDLQNTELSKLLPKQKELLRIQYAKIDALKAEEEAK
                     TQQKEVDSFMAQQAQELDALRQSFATKNEIARQAYLQREAIIQRGIARGAILENEAAI
                     LRKQNEQQYADERVAIAKEEADRKRQIELDRLSAMGQLFGNLATLMNTQNRKLFEIGK
                     AAAIAQAIVNTATAVTNALAVPPFPLGLALAVTAGVAGAVQIATIQNQKFSGARAMGG
                     DVQAGNSYLVGER"
     gene            complement(6547..6861)
                     /locus_tag="PLY05_01105"
     CDS             complement(6547..6861)
                     /locus_tag="PLY05_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01929.1"
                     /translation="MPHLECGQYLIDLLHDAGTVAYNDGIAKRLSWVELKAWCDLTGL
                     RLCPFESATIMMLSAAYADMLNEATDPNCPSPMLPKMTQSKREQVAIGLKNALRSIGK
                     KR"
     gene            complement(6993..7364)
                     /locus_tag="PLY05_01110"
     CDS             complement(6993..7364)
                     /locus_tag="PLY05_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01930.1"
                     /translation="MDLLQLLPSDYFELTLKHPVSGDELDAKIKVYGKDSSVFQNAIK
                     ARAKAQIARKSKDIDLESNEKDSIELLADCTKDWQGISEGGKQIECTRQNAIDIYSKY
                     KWIREQVDIAIGDRANFFINA"
     gene            complement(7411..7866)
                     /locus_tag="PLY05_01115"
     CDS             complement(7411..7866)
                     /locus_tag="PLY05_01115"
                     /inference="COORDINATES: protein motif:HMM:NF020392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HNC01931.1"
                     /translation="MTNSIVQTSAGTTIAISAVLPATDDAAGYAALTWAAIGEVTDLG
                     EFGREYATVTHNPVASRRTIKRKGSYNDGTMTLQLALDRDDAGQILAKTALGSDANQA
                     IRLTYQDGSKDYFSAMVMSFKTNVGSVDQILSGSINLEINTDIIPVALP"
     gene            complement(7878..8279)
                     /locus_tag="PLY05_01120"
     CDS             complement(7878..8279)
                     /locus_tag="PLY05_01120"
                     /inference="COORDINATES: protein motif:HMM:NF024943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator-like protein"
                     /protein_id="HNC01932.1"
                     /translation="MSQAQIELALFDKLEAIKASLPTIYYPNSPNKNKPNPPTGEHIR
                     VSVLNAGTNAIGIATTDQTLGIMQCLVLVKDGTGTVRAAQIADLILSAFARNTLLSNN
                     VRIDKTGSVGVGFTQDGWYMLPVSIPYQQIK"
     gene            complement(8276..8662)
                     /locus_tag="PLY05_01125"
     CDS             complement(8276..8662)
                     /locus_tag="PLY05_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01933.1"
                     /translation="MSFNDDIQKLARKLAITQKKAVAAFCINISRRVDNMSPVDTGLF
                     RANWQATLDQPYTGAVKTANRIGSIDHVIPFAKAANGHVFYLTNKLPYAQALEYGHSA
                     QAPNGMVRVSARMALQELENAVRSVQ"
     gene            complement(8662..9006)
                     /locus_tag="PLY05_01130"
     CDS             complement(8662..9006)
                     /locus_tag="PLY05_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01934.1"
                     /translation="MAINYANLAALSERLIRENGRDALLVTETNTGTDYQPTISQTSE
                     TIKLVQSSFTSNDNNDFVLQAHDIKFLVSSAFTVSAKQRIETNGLQYSIVAVKEIKPS
                     DTGILYLVQGRL"
     gene            complement(9006..9449)
                     /locus_tag="PLY05_01135"
     CDS             complement(9006..9449)
                     /locus_tag="PLY05_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01935.1"
                     /translation="MTVTIGYTTDDDFIAFALARGVTVTAPNAAIALTKALDYMETRQ
                     YKGYKTDNDQVLDWPRQYVYIDNVLLDSATVPSGIVKAQHVIALSIANGYDPLATIER
                     AVKREKVDVLEVEYQPNASATPILRSINAALSDYVASATSVMRSL"
     gene            complement(9511..10665)
                     /locus_tag="PLY05_01140"
     CDS             complement(9511..10665)
                     /locus_tag="PLY05_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004834133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HNC01936.1"
                     /translation="MSNTLTGLIPDLYAALDVVSRELVGFIPAVTTDAQVNRAAVGQN
                     VTVAVAPDSNSMVDTTPAMSVPSAADQTIGSVSVQITKSKAVPFSWEGNEQVGLNSGA
                     GYLTIRANQIAQAMRTLANAVELDLAALYATTSRAAGTVGTVPFITNTAALSAARKIL
                     VDNGAPTSDLQLVIDTNAGANLHTLYNINSSRDAAAQSLAQQGVLAMPSGVSIRESAQ
                     IYNPTSGTASGATTNNAGYAVGATTITLASAGTGTILAGDVITFAGDTNQYNVVSGDA
                     DVSNGGTITLAAPGLRKAMSAATKAITVLATSPRNMAFSRSAIVLATRMPERPQEGDM
                     AIDVMTIQDPRSGLAFEVAMYPGYRKVRYEIALAWGVKNIKPEHTATLLG"
     gene            complement(10677..11375)
                     /locus_tag="PLY05_01145"
     CDS             complement(10677..11375)
                     /locus_tag="PLY05_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01937.1"
                     /translation="MIEVKDLNDVPEKYRADYIEVEKDGVKIFQHKDFLTVIGAMKRK
                     GEERDTLANELKGYKSKEAERVAEAERKALEKLKAEGKIDEILADNEKRHGETIKQYE
                     ERIAKRDAIVIKKARDSVVNELSALATEAGAKAFKKLISERVNYDPETDKYTFTDEDG
                     GATSLDLAGFKADILKSDTYAAMLKATVSSGGHGKNVLNGGGAAKTITRAQFDAMNPN
                     QRAEHFKNGGTITN"
     gene            complement(11399..12850)
                     /locus_tag="PLY05_01150"
     CDS             complement(11399..12850)
                     /locus_tag="PLY05_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HNC01938.1"
                     /translation="MTKHTAHNDYIIAQDELFLIRKFIQGEAAVKREGVKFLPHPNQL
                     ECNTPEQIRRYEAYKMGAELEDFPSRTLNDLLGAMNRHPAKVNLPEKLQYLIDDSDGD
                     WLSLQASIEITASNCLQVGYHILLAEYDQLPSGLDVELSIADKAALNQRASIKHYPRE
                     SLKDWAWGKFNGRLTFTYMRLESEEIRKDANGTNINVTVCLELGIDEQGYWQELEVYN
                     GQASIQKEERIYPKAMGKNLTYIPVEVVQTERRLAGKLQIEGGYLAPLCHKAHARYQV
                     SADLKERLRILQDTSYSSGWDESKKEEFDTINGRKYFAFGAGVHNFLPDGVNMDILKL
                     TADGDALFRYMDENAKQIRAIGGRFDTQDKSQETLGEVEIKDANEKSVLTMLTNNIER
                     AYKNIIAYCGEFEGLALMPSDVDLVLNREFTSTKLTPDEVRAIRELVMDRLMTPQMAI
                     EKLIAGGYLSGEAEEIIGMIESMPLPVAVNGVQ"
     gene            complement(12847..14055)
                     /locus_tag="PLY05_01155"
     CDS             complement(12847..14055)
                     /locus_tag="PLY05_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01939.1"
                     /translation="MLTIDSALRHELTVSRLVTGIVQSRIMPSYFDLSQAVKVALADY
                     EPTMSRKDFDLLRRQVSLMVAEKMNAMWEGTTNDLFDLAKYETNHVVGELSGGTAVSE
                     AAISKAVNSPMVLAGATVASVGTWREYIAGATNSTQARIIDNTIRAGYESGATVQEMT
                     KRLIGTKANNYLDGLVTNTGAREAEALVRTGANHYANQARDVAAQANADLIQGRIFLA
                     TFDNRTTLTCRHFGQLQKIYALDDPATPKPPLHFNCRSVLSLVPIGFDPFSGTKASVG
                     GQDGQQAEEAYNKKNDRLDARREKADEQRAQGKEPKDVPSKVTYSGRKDSAIFKAGQI
                     DAKTSMDSWLRSQPDWFIESALGKTRAKLFKEGGLSIEKFTDMNGRPLTLKEMKALDE
                     FDAAFRKAKL"
     gene            complement(14055..15308)
                     /locus_tag="PLY05_01160"
     CDS             complement(14055..15308)
                     /locus_tag="PLY05_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HNC01940.1"
                     /translation="MRTLKIRATNPQAQFLAMTAKYRLFCAGFGAGKSEAMANAAMID
                     ACESSDTLIGLYAPTYDLVRLITAPRITAKLTEHGISYNYNKSENVIYTSAPRFGDFI
                     LRTLDNPERIVGYETYKAHCDELDTLQTEHARHAWNQVIARNRQRPNGIIEPFNQANA
                     YTTPEGFRFCHERWVAKKTDSYDMVQAASYTNPFLPPDYIESLKESYPASLVDAYIEG
                     RFVNLTSGTIYKNYDRKRCNSNETIKTGEPLFIGQDFNVGAMASTIYVKRVNGWHAVD
                     QLTGIFDTPELCQVLKERYAGHKITIYPDASGNSRKTVNASESDISLLKQAGFTVKVN
                     SSNPRVKDRILAVNGALANGKMWVNASKCPDVVACLEQQAYDKNGEPDKNGGFDHQND
                     ATGYPIVYEMPVRRPSASGINMSGF"
     gene            complement(15280..15594)
                     /locus_tag="PLY05_01165"
     CDS             complement(15280..15594)
                     /locus_tag="PLY05_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01941.1"
                     /translation="MVIDDNNSRKVGQSAAGNRGKGRIKGVPNKTTKAVKDALQEAFD
                     GLGGVDALIVWAKTEQTEFYKLWTKLLPTEVKAQVKNIGDTPIGKVQIEVINANTQDS
                     SD"
BASE COUNT         4043 a         3532 c         2916 g         5083 t
ORIGIN      
        1 cactagcatt tggatattcg ttttgtaacg agtaaattgt tcgcctgctt gcacctaatc
       61 ggtctgttac tgtgtttgct gtactattag caattcctgc aattgtatca acgtctaatt
      121 ttgcattctg taaatctgtg actgttaatt gagtcataat acatcctcac gattcgggat
      181 agtttctaaa tcatcactat aataatttgc atcatagtta attgcactga cttttacata
      241 tgatttgtca gatatattaa cttcttgcac aagataactg tttgcacttt ctgcactgtc
      301 agcagcaaag ctaaatatcg ttctaacacc atttgctcca ccatgtgtcg tgtttatcgc
      361 ttcgcttggt gcgttatcta aaacaacttg attcgcttgt gtacctgccg tacagctgat
      421 tgactcaagt gaaccatcgc gtttcattag taaaatactg tgagtaccaa caccaaaaac
      481 aacatcacgc gacagcgtaa gcgttaggcc gctttgtgca ataacttcgc catcttggct
      541 atcaaaacgg gtattatcga caatatcaat acgctgatta ggcaacaata gccgaccatc
      601 tgttgttgtc tctgtttcaa tgctaatacg ctgatatttt agtttgttgt actcacgcat
      661 tgcgcgtata taagcaacat caaaaaatct tacaccaact aagtctattt ttttatagtt
      721 tgttgcgctg ccgtctgttg gtattttaat tgtttcttga gcatcactaa catcatcatt
      781 gtatgttaga tcaatgccgt catattcatc atcagccgcg aatcttcggc taataacatc
      841 actagcaggt tttttattcc tgtgtgtaaa taacgcggta cttgctgttt gtggtttatc
      901 aaaactaaat ctaattttac cattttgacg ataagcaata caaaatacag cgtttgcaat
      961 cattcgtatg gtttcttcat aactgatatt gtcactatca agcgtataat gaaaagccat
     1021 cggctcagta cctaatccaa aataaccacc tatttgacta tacaaagcgt aaatttgggc
     1081 aatgtctatt gttgttaatg attgtcgtcc tatgaatggg tcttgtgtga ttgcagctag
     1141 aatatttaca aatgatgttg ttgctgcaat cgtcccactt aaaacactgc cgtcggtatc
     1201 aaaaacccca ctccatgttg tgccgttata tgtcggtaac tttctagtaa ctaaacaatt
     1261 tagtttacgc tcttttaatg ataatgcgcg ttgagttgct ttagttatca cttgaactgt
     1321 tgttacattg ccaaaatcgc tattagtaat cagtgtaaca gcacttaatg attcgtactt
     1381 tatctcatca acaacattgc ctgcaaaacc aaaatcatgg tcatttgagc gtctaactct
     1441 aactcttgtt gcacctagcc aacctgttgt tatctcgatt gttttagcaa cttgtgaagg
     1501 tgattgtccg ctcatgctgc cgctatttgt ataaatactg cctgttggtg tgccgctaac
     1561 tactttttga tattgtattt cataattaac agttaaattg tagcttccct cgctatcttg
     1621 atagtacaag ccctgttgag ctattaaatt aacccatatt tgggtcatat ctgtatctgt
     1681 taaagttacc cagtctgtcc attcaggatt tcctgttgtg acagtgatag tcgcatttgt
     1741 tgttgctgta gccgaaaaag tagcagttgt tagctctagt atatttgttc cgcctaattt
     1801 gtttctaact gtatataccc cgttatatcc tccaatactg ccagatattg ttaaaacatc
     1861 accaatagag atattgtcat ataattcatt gctaataccc cctattctgt cgtaagaact
     1921 cggtatcgta gatgctttgt aaaaatctaa ataaccagta gtctctgcaa gcgaaaactg
     1981 gttacgcgct tgcaataccg caccgtcaac attgtttgat tttttaacag tcaaaacagg
     2041 ctcactgatt gaaccgccaa tcgttaaaaa tgggctgccg ctattgggtg aagtaaaagg
     2101 attaaaaaac tcggcttttg tccctgtaat ttcatttagt ggcgtttcac catcacgaac
     2161 atcatcaact gcataccacc cgcgaccaat gcacatatag ctgtattcat actgcacatt
     2221 atcaatgtat tttgagtaaa cgggctgcaa aaggcttgga tatgcgcgta cttggccgta
     2281 aatatcttct atgcgctgca atactctcgg ctcgtttgtg cgccctgcta gggcattgtt
     2341 cgggctttgt tgtgtgcggt ttaggttgct tggcgttttt aaattagata attggtaaag
     2401 tgtgtagcca gcggtcaaaa cagtaacaac aacacttatt gccacccaat gagctacaac
     2461 aaacgcaaca acaggagcag aaggcgtaat caaaacaaca tactcgcccg tattattcat
     2521 tagcgcgttt acgttttttg taatatctgt ctcattgctt ggctgaccgt taaaaacagc
     2581 aaaattaaca agtctttttt tattatctaa aatccattct gctacgcttg agtattcgac
     2641 aatctcaggc tcaatcgctt taaaaacatg gtcataaata ctaattttta cagtcataac
     2701 gataatactc aatgagtccg taaacatcgg ctatttgtgt caagggctgc catatcacca
     2761 tatcacgcaa actatgcagg actccctgat tataaaacaa tccgcaatgc gtgaccttct
     2821 tatttttacc aagcaaaacc acgtctaaat tattcgcttt ttcggtcttt gcaaaaccgt
     2881 gtttgttatt gtgtaaagca attctaaacg cattagccac gtctcgcatc gtgttacttt
     2941 ttggtgtgta gtcatcaagt gatagcccta gctcattaac ataaacatca gcgactaact
     3001 gccaacatgg aggccactca taatgttttg caatgtattt atcaatcata aaaagccacg
     3061 caacattggg aatctactaa acgtataaag ctcacctgtt cgattaacat taagttttgg
     3121 tgcaatcgct gatattgtcg ctgtgccgcg attgtatgca atagactcta cttgtaaggt
     3181 ttgcacggct tgcggttcgg ttaaatcatc ggataaatag gcgcgatagg ttaagagtat
     3241 tttttctgtc gtgtctaacg gtattctgtc taactctttt ctcagcagat tgtcggggtc
     3301 tgttgtgtca atatttactg tgaatttttg gtctaagttg tcgggtgttc ctgccaatgc
     3361 tactgtaaaa ttagttgagc gcacagttag cacgttatca ttttcatcaa ccacgccacc
     3421 attgctcggc tcacgccata aatgatatgt ttgcgttaaa tcagagtgcg ctatgcttat
     3481 cacttcaatc atatagattg tttgtggcgc actcgctaaa aactctctta gtcttgcttc
     3541 aatatctaaa ctcatactag caccaacgta tcagataaaa caaactcgga caaacggtcg
     3601 aagaaatcgc ctaaaccccc taaatcccca taatcttccc aatacgatag tataaattct
     3661 gcacccccat cggtaaaatc gtaaatctta gattctgctt ctacttgaaa agtcactaca
     3721 aaattattgc catccgtttc ggttgtgttt acagagtttg ggatgatatt cactaaatgc
     3781 tgctctagtc ctgtgccgct atctaatggc atctcaaacg ctaacgcgcc ttttttaata
     3841 atgcgtaaat aaaataactc ccaaactagc aaatgacctg ccgtacacgc taaggcaaca
     3901 ttataaaccc ttgtgcctct gtcaaaatcc attgcataac gattaaagcc cccttcgact
     3961 tgggtcatgt gtacgccttg agccgatgcg taactatagc cgcttggcga agtaacaggg
     4021 tataaatcac gcggtaaaac tggggtaggc atggtttaac gtctccgttg taagccaaac
     4081 gcggctgctt ggtttcggct aatcttgcta tttgggtcta atgtttgcgc tgcgactgct
     4141 tcaatgactg ttaaaatcaa ctcaccatca ggcattctgc gctcttcgac tttatcaatt
     4201 ttgccagttg tttggttgac aatcgttact ttagtatcac cgcctaattt gtggttaggt
     4261 gtcacatggc cattgccacc cattgtaata atttccgcgc cacgttcacc cactaagtaa
     4321 ctattccctg cttgtacatc gccacccatt gcgcgnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnncacgt tcacccacta agtaactatt ccctgcttgt acatcgccac
     4501 ccattgcgcg cgcgccgcta aacttttggt tttgaatagt tgcaatctgc actgcccctg
     4561 ctacccctgc tgtcactgcc aatgctaggc caagtgggaa tggcggcact gctaaagcat
     4621 ttgtaacggc tgttgcggta ttaacgattg cttgagcgat tgcagcagct ttgccaattt
     4681 caaatagctt tcggttttga gtattcatta gcgttgcaag attgccgaat aattgaccca
     4741 ttgcgctaag tctatcaagt tcaatttgcc gctttctgtc agcctcttct ttagcaatag
     4801 ccactctttc atcagcatat tgttgttcat tttgtttgcg aagtattgca gcctcgtttt
     4861 ctaaaattgc accacgagca atgcctcgtt gtatgattgc ttcacgctgc aaataggctt
     4921 gtcgtgcaat ctcgtttttt gtcgcaaaac tttggcgtag cgcatctaac tcttgcgctt
     4981 gttgtgccat aaaactatca acttcttttt gttgtgtttt tgcttcttct tcggctttta
     5041 atgcgtcaat tttggcatat tgaatccgta ataactcttt ttgttttggt agtagctttg
     5101 ataactctgt attttgcaag tcaaagttaa gttttgccaa ctctgactca ttaccccaca
     5161 attcaaccct ttcttttgct gacaacaaaa gagacttata cttttcttgt aactggtcag
     5221 catctttaga ttgttgtttt ttgttttttt ctgcctcttt ttcggcttga agttgttttt
     5281 ttctttcttc aaaaagctct tttgttgatt ttcttgtgct ttcgtttatc caatcatcaa
     5341 gcagagcgtc aagttctttt tgttttctta actcctccct tgctttgcgc tcgtcttgaa
     5401 tcgctttaac tgccgtgttt cctgcaccta aacgcgcatc acgctcggct aaaatagcag
     5461 ctatagaatc cttgcgctgt ttgtctagct cattccaacg ctcggcacta ataatcaatc
     5521 cttctttgac ataatcaaaa aatgacttaa cttcgacttg tgcaattttg aatgatgttg
     5581 caatgttgac tggcagcttt gtaaaagcat cagataaaaa tgatgcagta tcaccgccat
     5641 ctttgcgtaa tacgcccaag tattttaata aatcaacagt gaccgatgac acatcatcga
     5701 cagcataact ccacaacgta aactgataag tcagtgtctc tagtggctgt ccactatcaa
     5761 tccaagctgt aataccctcc aatgacgaca caagcgaatt acttgcacct gtggcaacat
     5821 ctaattgacc aacaaagaca atcatggcgt ttttaagttt ggttgatgct tggccgattg
     5881 ttgcacttgt ctttgatgct aattcatcaa caacgcttga ttgctcttgc aatgcactga
     5941 ttaaaatatc tgtcgtaagc attccctctg ctgcaagttt gcgtaactcg cctttgctta
     6001 cacctaatgc gcgactgaat gcgtccatcg cggcaggtgc gttttctgcc acgctgttaa
     6061 attcatcacc gcgaataacg ccacttgcca acgcttggct aaattgcaaa atagccgctt
     6121 ctgcttgttg tgttcctgcc cctgatattg ctaatgtttt tgcaaatgtt tctgtgactc
     6181 gcgatacatc actaacactc aatcccaatt tatcagcgtt ctgactaatt ttaaaatata
     6241 agtcacctgt tgcccccaat gcttgatttg tgcgtgatgc aatttgatat acatcatcgg
     6301 ttgcgtatgc aagtgcctct gttgaatctg taactaactt tagcttgttc tgtaattgag
     6361 tgtaagagtc tgcatattct atgattttgt gaactgcaaa agcactggcc aaagttgcag
     6421 ccatgcgacc aagtgcggcc tctgttttgt tacctgtttt ttgtagatta tttaatgcgc
     6481 gctccccatc gcgtaatcgt ctagtatcaa cacctagccc aatcattagt aaatcagcca
     6541 tgttttttac ctctttttac cgattgagcg taacgcattt tttaaaccta tggcaacttg
     6601 ttcgcgcttt gattgtgtca tttttggcaa cataggacta gggcaatttg ggtctgttgc
     6661 ttcgtttagc atatcagcat atgcagccga taacatcata attgtagcag attcaaatgg
     6721 acaaagccta agccctgtta agtcacacca tgcctttaat tctacccatg acagtctttt
     6781 tgctataccg tcattataag caactgtgcc agcatcgtgt aataagtcga taagatattg
     6841 accgcactct aaatgcggca ttattggtgt gtcgtttgga ttggattgtt taaatgattg
     6901 aaggcgcgat atgcggttgt attctttcgc gcctttagtt tgtgggcaac tattccacca
     6961 tgcttgttgt ctgacgtaga gttttaacgg ctctacgcat tgataaaaaa atttgcacgg
     7021 tcaccaatgg caatatcgac ttgctcacga atccatttgt atttactgta aatatcaatg
     7081 gcattttggc gagtacattc gatttgtttg ccgccctcgc tgattccctg ccagtctttt
     7141 gtgcaatcgg ctaatagttc gatgctgtct ttttcgtttg attctaagtc aatatcttta
     7201 gactttcgtg caatttgagc ttttgcacgt gctttgattg cgttttgaaa tacgctcgaa
     7261 tctttgccgt aaactttaat ttttgcgtct aattcatcac cgctaactgg gtgctttaat
     7321 gttagctcaa agtaatcgct tggcaataac tgcaataaat ccatgataaa acctttttat
     7381 aattgcccct ttttatcggg gcatggttaa ttaaggaagc gcaacaggaa taatatcggt
     7441 gttaatttct aaattaatag aacctgatag aatctggtca acactgccaa cattagtctt
     7501 aaacgacata accatagcac taaagtagtc ttttgagcca tcttggtatg tcaaacggat
     7561 tgcttggtta gcatcagagc ctaaagctgt ttttgcgagg atttgccctg catcatcacg
     7621 gtctaacgcc aactgtaaag tcatcgtacc gtcattataa gagcctttac gtttaatcgt
     7681 gcgacggctt gccacagggt tatgcgtaac tgttgcatat tcgcggccaa actcgcctaa
     7741 atcggttacc tcgccaatag ctgcccatgt taaagctgca taacctgccg catcatcggt
     7801 tgcaggtaat accgcactaa tggcgatagt agtgcctgcg ctagtttgta caattgaatt
     7861 tgtcatataa gtgacctcta tttaatttgc tgatatggga tagacacagg caacatatac
     7921 catccgtctt gagtaaaacc aacgcctaca ctgcctgtct tgtctattct aacattgtta
     7981 gataataacg tatttcttgc aaatgcgctc aaaattaaat ctgcaatttg cgcggctcta
     8041 accgttcctg ttccgtcttt tactaatact aaacattgca taatgcctag cgtttggtct
     8101 gttgttgcaa tgccgattgc gtttgtacct gcgtttaata cactaacacg aatgtgctcg
     8161 ccagttggtg gattaggttt gtttttgttt ggcgaatttg gataataaat tgtaggtaat
     8221 gatgccttga ttgcttctaa tttatcaaag agtgctaact caatttgcgc ttgactcatt
     8281 gcacactcct gaccgcattt tctaactctt gcaatgccat tcttgcgcta actcgcacca
     8341 tgccgttagg ggcttgtgcg ctatggccat attctaacgc ttgagcataa ggcaatttat
     8401 tagttaagta aaacacatgg ccgttagcgg ctttagcaaa aggtataaca tggtcaatac
     8461 ttccgattct gttagctgtt tttaccgctc cagtgtacgg ctggtctagt gttgcttgcc
     8521 aattcgcacg aaataaacct gtatcaactg gactcatatt gtcaacacgt ctgctaatgt
     8581 taatacaaaa tgctgctact gccttttttt gtgtgatagc aagtttacga gctaattttt
     8641 gtatatcatc attaaacgac attataaacg cccctgcaca aggtataaaa tacctgtatc
     8701 gctcggttta atctctttaa ctgcaacaat gctatattgc agcccgtttg tttcaattcg
     8761 ttgttttgca ctaactgtaa acgcgctaga cactaaaaac ttaatatcat gcgcttgcaa
     8821 cacaaaatca ttgttatcat tgcttgtaaa tgagctttgc acaagtttaa ttgtttcgct
     8881 cgtttggcta attgttggct gataatctgt gcctgtatta gtctctgtaa ctaacaaagc
     8941 atcgcgccca ttttcacgaa ttagacgctc agataatgcc gctaaattag cgtaattgat
     9001 agccattaca acgacctcat aacgctggtt gcacttgcca cataatcaga taatgccgcg
     9061 ttaattgagc gtaaaatagg tgttgcgctt gcgtttggtt ggtactctac ttctaaaacg
     9121 tccactttct cacgttttac tgctcgctcg attgtggcga gtgggtcgta accattagca
     9181 atagataaag caataacgtg ttgcgcttta acaataccgc ttggtactgt tgcactatct
     9241 aacaatacat tatcaatata gacgtattga cgcggccaat ctaacacttg gtcattgtct
     9301 gttttataac ctttgtattg cctagtttcc atatagtcta gtgcttttgt gagtgcaatg
     9361 gcggcgttag gtgcggtaac agttacgcca cgcgctaagg caaaagcaat aaagtcatca
     9421 tctgttgtat agcctattgt tactgtcatg ttttatatca atccaaaaca atcacaatgt
     9481 aaaaaggggc ggttaagccc ctttatcaga ttaaccaagc aatgttgcag tatgctcagg
     9541 tttgatgttt ttaacacccc atgctaacgc aatctcataa cggactttgc ggtagcctgg
     9601 atacattgcc acctcaaacg ctaagccact gcgtgggtct tgaatggtca tcacatcaat
     9661 agccatgtca ccttcttgtg gacgctcagg catacgagta gccaatacaa tcgcgctacg
     9721 gctaaatgcc atgttacgcg gcgaagtagc caagactgta atggctttag tggctgcgct
     9781 cattgcttta cgcaaaccag gggcggccaa agtaatagta ccaccattgc ttacgtcagc
     9841 atcgccactt accacgttat actgattagt atcacctgcg aaagtgataa catcgccagc
     9901 taaaatagtg ccagtacctg cacttgctag ggtaattgtt gttgcaccaa cggcataacc
     9961 tgcgttattg gttgttgcgc ctgatgctgt accgcttgtc ggattgtaaa tctgtgcgga
    10021 ttcacgaatc gacacaccgc taggcattgc caatacgcct tgttgtgcaa gtgattgagc
    10081 tgcggcatca cgcgaagaat tgatattgta caaagtatgc aagtttgcgc ctgcgtttgt
    10141 atcaataact aattgcaagt cagatgttgg tgcgccattg tcaactaaga ttttacgcgc
    10201 tgcgcttaat gctgcggtat tagtgataaa aggcactgtg ccaactgtgc ctgctgctcg
    10261 gctagtagtt gcgtacaatg cggccaagtc taactcaaca gcattagcca gagtacgcat
    10321 agcctgtgca atttgattag cacgaatggt taaatagcct gcaccactat ttaggccaac
    10381 ctgttcgtta ccctcccacg aaaacggaac tgctttagat tttgtaatct gtacgctaac
    10441 gctgccgatt gtttggtcag cagcactagg cacagacata gcaggcgtag tatctaccat
    10501 gctattagaa tcaggggcaa ctgctactgt tacgttttgg ccaacggctg cacgattgac
    10561 ttgtgcgtca gtggtaacgg cagggataaa gcctaccaat tcgcgcgata cgacatctaa
    10621 agccgcgtat aaatcaggaa tcaaacctgt taatgtgtta gacataataa aaacctctaa
    10681 ttagtgattg tgccgccatt tttgaaatgt tcggctcgtt gatttggatt catcgcgtca
    10741 aactgtgcgc gtgtaatcgt tttagcagca ccgccaccat ttaaaacatt tttgccatga
    10801 ccaccgcttg atacagtagc ttttagcatg gcggcatagg tgtctgactt caaaatatca
    10861 gctttaaacc ctgccaagtc taacgaagta gcaccgccgt cttcgtctgt aaacgtgtat
    10921 ttatctgttt cagggtcata attaacccgt tcagaaatta actttttaaa cgcttttgca
    10981 cctgcctctg ttgcaagtgc agataattca ttaactacag aatcacgcgc ttttttgatt
    11041 acgatagcat cacgtttagc gattcgctct tcgtactgtt taatcgtttc accgtgtctc
    11101 ttttcgttgt ctgctaaaat ttcgtctatc ttaccttcag cctttagttt ttctaacgct
    11161 tttcgttcag cttctgctac tcgctcggct tctttcgact tatagccctt taattcgttg
    11221 gctaacgtgt cgcgttcttc gcctttgcgt ttcatcgcac caatgaccgt taaaaaatct
    11281 ttatgctgaa atatcttgac tccgtctttt tcgacttcga tataatcagc ccgatatttt
    11341 tcggggacat cgtttaaatc tttaacttca atcataatca aaacctttga cttgtttgtt
    11401 attgtacgcc atttacagcc acaggcaagg gcatactctc tatcatgccg ataatttcct
    11461 cagcttcacc gctaagataa ccacctgcaa tcaatttctc aatggccatt tgtggggtca
    11521 tgagtctgtc catcactaac tcacgaattg cccgcacttc atctggtgtc agtttggttg
    11581 acgtaaactc acgattcaac actaaatcaa catcagacgg catcaatgcc aaaccctcaa
    11641 actcaccaca atacgcaatg atatttttat atgctcgctc gatgttgtta gttagcatcg
    11701 ttaaaacgct tttttcgtta gcgtctttaa tctcgacctc gcccaatgtt tcttggctct
    11761 tgtcttgcgt gtcaaaacgg ccaccaatgg cgcgaatttg tttagcattt tcgtccatat
    11821 atctaaaaag cgcatcacca tcggcagtga gttttaaaat atccatattc acgccatcag
    11881 gcaaaaagtt atgtacacca gcaccaaaag cgaagtattt acgcccgtta attgtgtcga
    11941 attcttcttt tttgctttcg tcccagccgc tcgaatatga tgtatcttgt aagattctta
    12001 aacgctcttt taagtctgcg ctaacttgat aacgtgcgtg cgctttgtgg caaagtggcg
    12061 caagatagcc accctcaatt tgtagtttac ctgccaatct gcgctcggtc tgcactactt
    12121 cgacagggat ataagttagg tttttaccca tcgctttcgg ataaatacgc tcttcttttt
    12181 ggattgacgc ttggccatta taaacttcta attcttgcca gtagccttgt tcatcaatac
    12241 ccaactctaa gcacacagtc acattgatat ttgttccgtt agcgtctttg cgtatttcct
    12301 cagattcaag gcgcatataa gtaaaagtta agcggccatt gaatttaccc caagcccaat
    12361 cttttaaaga ctcacgtgga taatgcttaa tgcttgctcg ttggtttagt gcggctttgt
    12421 ctgcaatcga taattcaaca tctagtccgc tgggtaactg gtcatactca gcaagtaaaa
    12481 tatgatagcc gacttgcaaa cagttacttg ctgtaatctc gattgatgct tgtaatgaca
    12541 accaatcgcc atcgctgtca tcaattagat attgtaattt ttcaggcaaa ttaactttag
    12601 cagggtggcg gttcattgca cccaataagt cattaagtgt acggcttggg aaatcctcaa
    12661 gctccgcacc cattttgtac gcttcatagc gtctaatctg ttcgggtgta ttacattcta
    12721 attggttagg gtgcggtaaa aacttaacgc cttcgcgctt tactgctgcc tcgccttgaa
    12781 tgaatttgcg aattaaaaac aattcatctt gtgcgataat gtaatcattg tgcgctgtat
    12841 gctttgtcat aatttcgcct ttctaaatgc tgcatcaaat tcgtctagtg ctttcatttc
    12901 tttaagtgtt aatggccgtc cgttcatgtc tgtgaacttc tcaatagata gcccaccctc
    12961 tttaaacagt ttagcacgag ttttcccaag tgccgactca ataaaccaat caggttgtga
    13021 acgtaaccaa gaatccatac tcgtcttagc atcaatctga cctgctttaa atatagcaga
    13081 atctttacgc cctgagtatg taactttgct cggcacgtct ttaggttctt tgccttgcgc
    13141 tcgctgttcg tctgcttttt cacgtcttgc gtctagtcta tcattctttt tgttgtatgc
    13201 ttcttcggct tgttgtccat cttgaccgcc cacgcttgct tttgtaccac taaatgggtc
    13261 aaaaccgatt ggcactaacg acaacacact ccgacaatta aaatgcaaag gcggcttagg
    13321 tgtagcaggg tcatcaagcg cataaatttt ttgtaattgc ccaaaatgcc tacacgttaa
    13381 agttgtacga ttatcaaacg tggctaaaaa gattcggcct tgtattaaat cagcgtttgc
    13441 ctgtgctgca acatcacgcg cttggttagc ataatggttt gcacctgttc taaccaatgc
    13501 ctcagcctca cgcgctcccg tatttgtgac taagccatct aaataattat ttgctttagt
    13561 gccaattagc cgcttagtca tctcttgcac tgttgcgcct gattcgtaac cagctctaat
    13621 agtgttatca ataatacgcg cttgtgtgct atttgttgcc cctgcgatat attcacgcca
    13681 tgtccccacg cttgcaactg ttgcacctgc taaaaccatc ggactattta cggcttttga
    13741 gattgcagct tcactaactg ctgttccgcc tgataactca ccaaccacat gattagtttc
    13801 atatttcgca aggtcgaaaa ggtcatttgt tgtgccttcc cacatagcat tcattttttc
    13861 tgcaaccata agcgagactt gccgccttag caagtcaaag tctttgcggc tcatggttgg
    13921 ctcataatct gccaatgcaa cttttacagc ctgtgacaag tcaaaatagc tcggcataat
    13981 gcgtgattgc actatacccg tgactaatct tgacactgtc aactcgtgac gtaatgcgct
    14041 atctattgtt agcattaaaa tccactcatg ttaataccgc ttgcgcttgg ccgtctaact
    14101 ggcatctcat aaacgattgg ataaccagtc gcgtcgttct gatggtcaaa gcctccgttt
    14161 ttgtcaggct ctccgttttt atcatacgcc tgttgctcta aacacgctac aacgtcaggg
    14221 cattttgacg cattaaccca cattttacca ttagctaacg cgccattgac ggctaaaata
    14281 cggtctttaa cacgcggatt gcttgagttt actttaaccg taaatcctgc ttgttttaac
    14341 agtgatatat cactctcgct tgcgtttact gttttgcgac tattaccgct tgcatcgggg
    14401 taaattgtta ttttgtgtcc tgcgtaacgc tcttttaaga cttggcataa ctcaggcgta
    14461 tcaaatatac ctgtgagctg gtcaacagcg tgccaaccgt tcacgcgctt aacataaatt
    14521 gtcgaagcca ttgcacccac gttaaaatct tgcccgataa acaacggctc acctgttttt
    14581 attgtctcgt tgctgttgca tcgttttcta tcgtaatttt tataaatagt gccgcttgtt
    14641 aggttaacaa aacgaccctc aatataagca tctactaaac tggcaggata agactctttt
    14701 aatgactcga tataatcggg tggtaaaaaa ggattagtgt aagatgcagc ttgaaccata
    14761 tcataactat ctgttttttt tgcaacccat cgctcgtggc agaatctaaa accttcgggt
    14821 gtggtgtaag cattagcttg gttaaatggc tcaataatcc catttggtct ctgcctatta
    14881 cgcgcgatga cttgattcca agcgtgtcta gcgtgttctg tttgtaatgt gtctaattca
    14941 tcacaatgcg ctttgtaagt ctcataaccc acaatacgtt caggattatc aagcgttctt
    15001 aaaataaagt caccaaaacg cggcgcgctt gtatagataa cattctcaga cttgttatag
    15061 ttgtatgata ttccatgttc agttaatttt gcggtaatgc gtggcgcggt aataagccgc
    15121 actaaatcat aagtaggggc atacaatccg attagtgtat cactagactc acaagcatct
    15181 atcattgccg cattggccat tgcctcagat tttccagctc caaacccagc acaaaaaagt
    15241 ctatactttg cagtcatagc taaaaattgt gcttgcggat tagtcgctcg aatcttgagt
    15301 gttcgcattg ataacttcta tctgtacctt gccaatcgga gtgtcgccaa tgtttttaac
    15361 ttgtgcctta acttctgttg gtaataattt tgtccataat ttataaaatt ctgtttgttc
    15421 tgtcttagcc catacaataa gcgcatctac tccgcctaac ccatcaaacg cttcttgtaa
    15481 agcatctttt actgcttttg ttgttttgtt cggtacgcct ttgattcttc ctttacctct
    15541 atttcctgcc gcactttggc ctactttacg ggaattatta tcatctataa ccataagaac
    15601 ctcttataat cacacccatt taacaaacac ccacaacaac aaaaacggaa agctcaccat
    15661 tgcaattata gtat
//
LOCUS       DAOQMB010000013        15482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1378_length_15482_cov_16.426979, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000013 DAOQMB010000000
VERSION     DAOQMB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 15482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15482
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1378_length_15482_cov_16.426979"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /gene="gmk"
                     /locus_tag="PLY05_01170"
     CDS             <1..525
                     /gene="gmk"
                     /locus_tag="PLY05_01170"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNC01942.1"
                     /translation="KVSISNTTRNKRAGELDGVHYHFTAKEQFVDMISQGAFLEHAEV
                     FGNYYGTARHMVEENLRQDIDVILEIDWQGAQQVRQSYPNAVSIFIMPPSRDALRQRL
                     ENRGQDSADIINQRLNGAISDMSHFVEFDYVVINDDFDIALEDLIAIVKASRLRQNIQ
                     VIRHADRIQGLLSN"
     gene            621..878
                     /gene="rpoZ"
                     /locus_tag="PLY05_01175"
     CDS             621..878
                     /gene="rpoZ"
                     /locus_tag="PLY05_01175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HNC01943.1"
                     /translation="MARVTVEDCLGAVENRFELVLLAAKRARQLATGNKEPFLPWDND
                     KPTVMALREIAEGYVDRHLLSQKVEEERDNEPYLALDTSTF"
     gene            933..3044
                     /locus_tag="PLY05_01180"
     CDS             933..3044
                     /locus_tag="PLY05_01180"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC01944.1"
                     /translation="MNTGIDVLCQHLNSYLAEDQIAAVRAAYFFAERAHGSQMRRDGT
                     PYITHPLAVADILSQMHMDHQSLMAAMLHDVIEDTGISKSELAKMFGDEVAELVDGVT
                     KLEKVGHISKAEAQAENLRKMMLAMTRDIRVIIVKLADRLHNMRTLDVLKPEKRKRIA
                     QETLDIYAPLANRLGMNDVRVELEDLCFFALFPLRAPLIQKAVKVSYQNRQETMAFIK
                     HSLETSLKAAGIHGRILPREKHLFSVYRQMKENHLSFHEIMDVYGFRIVVDTLDSCYR
                     TLGVAHNLFKPIPNKFKDYIAIPKVNGYQSLHSILKGKRGVPIEVQIRTESMEAMAKY
                     GVAAHWLNQDRVDNRAQAHAQEWMSHLLELQQNTGDSLDFVENVKTDLFPDEVYVFTP
                     KGRILTLPQGATSVDFAYAVHSDIGNSCVAAKVDGRLAPLSQILTTGQTVDIITAAGA
                     MPNPHWLNFVITSKARSNIRQFLKSQQQDQSVDLGRRLLEKSLAAYGTKLSKIDANIL
                     QLVLHELNFSTLDLLLEDIGLGNRAPQLVAQRLLPDNPQDASRHHKRRLAIKGTEGLV
                     LSYARCCYPLPGDSIFGHLSAGRGIVVHRDVCHHLINELRNNPDKCVPLQWAKEVIRD
                     FSSELAIEAVNQRGILAEITRALDLHESNIESINSKQDYDQGHATIIIGISVKNRIHL
                     AQIIKRLRILNGIEKVTRVKH"
     gene            3230..5965
                     /locus_tag="PLY05_01185"
     CDS             3230..5965
                     /locus_tag="PLY05_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycoside hydrolase 114/
                     polysaccharide deacetylase family protein"
                     /protein_id="HNC01945.1"
                     /translation="MVLSTNTYATSPNVALFYGANPPLNELRTFDIVVVDPDHANIEP
                     QTYNSEYSQLFAYASVGEVHRSKAYFQQIPKNWLKSKNQAWDSYVLDQSSPEWPNFFA
                     EQVIAPLWAKGYRGFFLDTLDSYQLISQTQQQRQVQEQGLINTIKTIKQRWPEAKLIF
                     NRGFEILPQVHDAAFMVAAESLYQSWNAGANQYQTVKSEDRAWLINQLQQVKNNYQLP
                     ILVIDYVQNAERELARQTASKIRQEGFIPWVSTGDLDSLGVGNLEVLPRKVLVIYNPN
                     EAPDLHYHDAVRFLGAPLAYLGLVPEFIPFNSQLPNKNLVGRYAGIISWINSDDIANN
                     SAYPQWLSQQIKQGLPVAIFSRFGFSAETGLFNQLGLRSVDVNSNTPLQVKHTDKMMG
                     FELPVVARAEELYGVQLQSATQPLLTLHNPQTQQNYHPAALTDWGGYVLAPYTVDVLP
                     AKEGGERWLINPISFLTKALKLDEQRPIADVTTENGNRLLMVHIDGDGFMSIAERATR
                     PFNGQVLLDDFFKRYQIPTTMSIIEGELGAEGLYPQQSPALEKIARDIYALPWIELAS
                     HSYSHPFFWSKAQQSADDTGDYESYHLPIKGYKYKVEREVAGSINYINQKLAPKNKQV
                     KVFLWTGNCVATPEALAETVKTGVLNMNGGDTTITRSNNSWTRIAGLGLKKGNNFQVF
                     APNQNENVYTNLWTGPFYGFERVIETFELTDKPYRFKPINIYYHSYLVSKTAGANSLH
                     KIYQWALKQPVLPIYSSEYIQKVLDFNDLAIARSPQGYRIRGNNQLKTLRFNHSKAID
                     FNHSPQLMGFNHHNQEQYLHLANNNLDINIQDTPSTLAYIESANGQISQFQRYLNSVT
                     FNIKSSQPLRVSFANSQKCVLKANGKVLKTRQQNSRLLLEVDAHELSSLRLSCTP"
     gene            5928..6833
                     /locus_tag="PLY05_01190"
     CDS             5928..6833
                     /locus_tag="PLY05_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01946.1"
                     /translation="MSLARSGFPALPERLRFATTGQILGFGLLTLLTFMVIYPEHTLQ
                     RHLERSARTDNVSIAYLFAWLKADASDHHLRILLAQRLFDKGEISQSRQVLNPIFKVK
                     ELSTEQESKANILLLDILERQLWTYQPQSATFNNALKQYLRQLRKISYYSWPQARLEK
                     FASNAYAFADYTLAKELYYQLIFSASAVNPQWFEKLAQLYMVQGQYRSAARSFFQAMP
                     YCTNLTQRKTFFLSGVKALQAGNLVNDALAAGQMYVGVFIHDAATIEFLARLALSANR
                     PDIAQYYVALLLKQRINSPSTGATP"
     gene            6830..9634
                     /locus_tag="PLY05_01195"
     CDS             6830..9634
                     /locus_tag="PLY05_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC01947.1"
                     /translation="MKWLYALGLCTLSLAASAETLDIAQEAGSPQAQFDDLRYRLGFD
                     VYLANKNLPAAWQVAKKAVSVEPNNLFWLERFAQVSEWLGKPEEALPIWLRYAQKTGQ
                     QSAWDNVKRLAIGLYDDKALLIYQQRQVGLKPNDAAEIETLVQIYERLGESVAGLDFL
                     AQLRHKSQAKRALLTAEANLAERAGQDERAINAYSQLISLFKPVDQTWLLRRAALWYQ
                     RGKLVEAWQSLSELEHHMPDRAQDYWHTYAELSRLLNKTDIAQRAYQKLTEEFLFVDG
                     DLINYAALETEKNPLNAAFLSELSYRLYKRDNAVLSLLYAYQKANYPQGIERFLSLLT
                     TEELQKFEQNPLFLEQRGQFYWQQKQLVKAQADFELALKLAPKQSRLLNALVGVLIEQ
                     NKRTDLNTVLQAAHRQAQRQAALWQVWANGWLFLQKPQQALPFQYAYYQTNPQDSLAG
                     LNLAELLANTGATQQAQALQQQIWQQRQQRIQQETDGRLQQLQQQLFALQLNQQTGDA
                     SQLLLAQHLRHHAYQQFEHEIYLGWLLNHQHNDRAQQLIAQHAHKSTPSWASLNLALQ
                     QQDQRQLNHLLSNQLANLPIYDRIEAAMRVNRADLAEDLAFNTQDDYPNDDELHRRYR
                     DLFSERGHSVDISHKQQELGALQRQNTTVSWTTPLNKDWRLQADIEQNQYQSLDQSIF
                     SNDLASQQRLAFTAMYHRQQQQISVQIQQWQHELGWAIKQQYKLDSRLSLGWQWQQQQ
                     TSEDSTALLVAGKKNRLAVNGTWNISGREYLLVDIAQDDYLTQYGDNLGSGQISQLQV
                     GHRLFADQLDHVLKLGYSLGQFSTNTQNLDQRLSSLTVAGQSLNSQFYIPQDYQQWSL
                     MWAFGQVNPQQYQRGWRVLGELGFNHANTNGLGFAGQLGIQGSIFGNDRLLLQLQRSQ
                     SGQQNGEQGQTWQLSYRLFY"
     gene            9649..10176
                     /locus_tag="PLY05_01200"
     CDS             9649..10176
                     /locus_tag="PLY05_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNC01948.1"
                     /translation="MSKFSLSALVIGLSLLQGCSTLHTTPKITKLDTSAQWVLVPSIN
                     NTETPQAGARLDSITASLLRVNGVNQLSVYQSNNNNESLFDLADRRNQEQALNAVKST
                     GAKYAVAASVDEWRYKVGLDGEPAAGVTVNIIDLSTGQVVWSGTAAKTGWSREAVSAI
                     AQKTVDQLLKNALSR"
     gene            10195..11550
                     /locus_tag="PLY05_01205"
     CDS             10195..11550
                     /locus_tag="PLY05_01205"
                     /inference="COORDINATES: protein motif:HMM:NF028273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PelD GGDEF domain-containing protein"
                     /protein_id="HNC01949.1"
                     /translation="MLHLFTRRQLAAVFAPKQAGRLMVVEIVLFTLATIAIGAWSNPK
                     DPFMVHLPFPWLWLAPTILALRYGSLAAIGSIGIISLSWSAWDTLSNNTELFPAHFFM
                     GGLILSLLAGEFADVWIVRLKRVREANGYLNERLESLTRRHYLLKLSHERLEQDMLTK
                     PMTLRDSLSHMRQVMIEGGFNTDKNPLPQAADLMNLLAQTCQLEVASLYSIHNDNINA
                     TAVTHLGSVHSFKADDPLVSYALEHKSLAHLQTEQLMDSHSQYVVVAPLIATNQQCFG
                     LLTVERMPFLSLNTETLQFLSVLLGYYADNVKVIPQALKILRDNPKCPLDFASELLRL
                     ERVQRESGLPSSIVAFVLSDSVHRQDIYAEMVRQRRQMDIAWDIRLSDRDILITLMPL
                     HGDAAVTGYLLRTQKWLKDMFNCSKFADAQMTPYAALVNERPAAELLNHLLERCLVKQ
                     S"
     gene            11562..12560
                     /locus_tag="PLY05_01210"
     CDS             11562..12560
                     /locus_tag="PLY05_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01950.1"
                     /translation="MLSTNGKLVTLAVSLELSAITLLFLSSNSTWLIYQYFFLHALAS
                     LAITPVAWTLMPANYKHPRLWVMFLLFSLSFFIPIIGLFGFAIGSLVASRLPYFNNTH
                     QIKIVHSPEYELPKHNNETGWRSARVRQQLTDKDTPVELRMKALLSLQNMPARHASSI
                     LREALSDSSDDLRLLAYGMLDSREKHITHRIQLALISYQKAISNQERYLPARELAELY
                     WELVYQNLVQGDMRNFALEQVQKYTTEALKEKVKDAGLWSISGRMWTIRGDYIRATGC
                     FSTAIKQGFPLARAEPYLAELAFLRQDYQTTRRIMKNLSERGGSRPLLMASDYWSK"
     gene            12562..14112
                     /gene="pelF"
                     /locus_tag="PLY05_01215"
     CDS             12562..14112
                     /gene="pelF"
                     /locus_tag="PLY05_01215"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HNC01951.1"
                     /translation="MAIIKQGQSADIGLLLEGTYPYVSGGVSSWVNQIIKGFPQYTFA
                     LCFIGSRPEDYGDMRFQLPDNVVHLETHYLHETHSKPPVIKRKGNPQVFKEIRCLHDL
                     YRHPEQMQQQETSLLNSIMNHRLAGRYAEDDFLYSKEAWDYITEQYRAHCTDPSFVDY
                     FWTIRIMHAPIWRLMALTESFIPVKAFHTISTGYAGFLGALMKQKTGKPLILSEHGIY
                     TKERKIDLFQAEWIRDNRGTLAKDSSEVAYFKNLWIRFFEALGRDCYHHADRIIALYE
                     TNRLRQIKDGAPAERTENIPNGINLPHLEKVRQNRSDTIPMTVCLIGRVVPIKDIKTF
                     IRAMRTVANQLPQVEAWIAGPEDEDPEYAADCHSLVNGLGLNDHVKFLGFQKIDELLP
                     KVGLLVLSSISEALPLVILEGYAAGVPCVSTDVGSCRQLIEGLGPIDKSIGESGIVVG
                     IANPTELAEAIIALLGDPERWHKAQAAGITRVERYYTQEQMFARYRSVYEQALNLSQQ
                     VTCPRGKR"
     gene            14112..15482
                     /gene="pelG"
                     /locus_tag="PLY05_01220"
     CDS             14112..15482
                     /gene="pelG"
                     /locus_tag="PLY05_01220"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HNC01952.1"
                     /translation="MAGIGFELRKLLRRDSLLGMLQAYAYAGIISSGPWVLSIVGILV
                     IGLMSAAIVVPEFLITQFQVSVTYLVALSLILTGFVQLAFTRFTADRLFEKRADIILP
                     NFNGLLFGVTAISGFLGTLAILFLFPQQSIMYRLLMLAGFVVLCDIWIATIFLSGMKQ
                     YKAILNCFAVGYGATVLFALLLRNYKLEGLLGGFVLGQFILLIGMVILILRNYPSSYF
                     IAFDFAKKRQFFWSLVWVGFFYNFGLWLDKFMFWFYEPTSQPIIGPLRASLIYDIPVF
                     LAYLSIIPGMAVFLVKMETDFVEFYDNFYNAVREGGSLEYMEKMRNEMVFTIRQGIYQ
                     IIKIQAITTLAVFVAGEIILNFLGISLLYLPLLFVNLISVGLQVVFLGILNVFFYLDK
                     RRIVLLLTALFVLFNGIFTAITLYLGPKFYGYGLAAALLVCIMIGFRFLDRVFEELEY
                     STFMLQ"
BASE COUNT         4508 a         3184 c         3070 g         4720 t
ORIGIN      
        1 aaagtatcca tttccaatac cacgcgcaac aaacgtgcag gtgaacttga tggtgtgcat
       61 taccacttca ccgccaaaga gcaatttgtt gacatgatta gccaaggagc gtttttagaa
      121 cacgccgaag tctttggcaa ttattatggt acagctcgcc acatggttga ggaaaactta
      181 cgccaagata ttgatgtgat tttagaaatt gactggcaag gtgcacaaca agttcgccaa
      241 agttatccca atgctgtgtc tatttttatt atgccaccct ctcgtgatgc tttacgccaa
      301 cgtttagaaa accgtggtca agacagtgca gatattatca accaacgact caatggcgcg
      361 attagtgata tgagccattt tgttgagttt gattatgtgg taattaatga tgacttcgat
      421 attgccctcg aagacttgat tgcgattgtc aaagcctcac gtttacgaca aaacattcaa
      481 gttattcgtc atgcagatcg cattcagggc ttgttgtcga attaatactc gcctacaatc
      541 aagggttcgc tactattatt cggacttacg cgctaattgt aatgagctgc gtaatcctgt
      601 cttgccaaaa tgaggtcaac atggctcgtg ttactgtaga agattgttta ggtgcggtcg
      661 aaaaccgctt tgaattggta ttgcttgctg ctaaacgcgc tcgccaattg gcaactggca
      721 acaaagagcc attcttacct tgggataacg ataaacccac cgtcatggct ttacgcgaaa
      781 ttgcagaggg ttatgttgac cgtcaccttt tatctcaaaa agttgaagaa gaacgtgata
      841 acgaacccta tttagcctta gatactagca ctttctaact ccaatgcaga ttaagggata
      901 acttccctta atctcccttt tttgagccaa cgatgaatac tggtatagat gtcctctgtc
      961 aacatctgaa cagttatttg gccgaagacc aaattgctgc ggtgcgtgct gcctattttt
     1021 ttgccgaacg cgcccacggc tcacaaatgc gtcgtgatgg tacgccttat attacccatc
     1081 ctttagccgt tgctgatatt cttagtcaaa tgcacatgga tcatcaaagc ctcatggcgg
     1141 ctatgctcca cgatgtcatc gaagatacag gcattagcaa atccgaatta gccaagatgt
     1201 ttggtgacga ggttgctgag ttggtcgatg gggtcactaa actcgaaaaa gttggccata
     1261 tttctaaagc cgaagcacaa gccgaaaact tacgcaaaat gatgttggcc atgacccgtg
     1321 atattcgggt cattattgtt aaattggctg accgtttaca caatatgcgt actttggatg
     1381 tacttaaacc cgaaaaacgt aaacgtattg ctcaagaaac cttagacatt tatgctccac
     1441 tcgctaatcg tttaggcatg aatgatgtgc gagtcgaact agaagacttg tgttttttcg
     1501 cgctatttcc gctacgcgcc cctttaattc aaaaagcggt taaagtttct tatcaaaatc
     1561 gtcaagaaac catggcgttt atcaaacata gcctagaaac tagtcttaaa gctgctggga
     1621 ttcatggacg tattctacct cgtgaaaaac acttatttag tgtgtatcga caaatgaaag
     1681 aaaaccattt gtcttttcac gaaattatgg atgtttatgg ctttagaatt gttgtggaca
     1741 cactagacag ttgttatcgt actttgggtg tggcgcacaa cctctttaag cctattccca
     1801 ataaattcaa agattatatt gccattccca aagttaacgg ttatcagtct ttacatagta
     1861 ttttaaaggg caaacgtggc gtgcctatcg aagttcaaat tcgtaccgaa agtatggaag
     1921 caatggccaa atatggggtt gctgcccatt ggctcaatca agaccgtgtt gataatcgcg
     1981 cccaagctca tgctcaagaa tggatgtctc atttacttga gttacaacaa aatacaggtg
     2041 actctttaga ttttgtcgaa aacgtcaaaa ccgatttatt tcctgatgaa gtttatgttt
     2101 ttacccctaa aggtcgaatt ttaaccctac cccaaggcgc aacatctgtt gattttgcct
     2161 atgccgtgca ctctgatatt ggcaacagtt gtgttgctgc caaagtggat ggccgtttag
     2221 caccattaag tcaaatttta acgacaggtc aaaccgttga tatcattact gctgcgggag
     2281 ctatgcctaa tccccattgg ctcaattttg tcattaccag taaagcgcgt agtaatattc
     2341 gtcagttttt aaagagtcaa cagcaagatc aatcggttga cttaggtcgt cgtttattag
     2401 aaaaatcact agcagcctat ggcacaaaac tgtcaaaaat tgatgctaat atcttacaac
     2461 ttgtactaca tgagctaaat ttttccacac tcgatttatt gttagaagat attggtttag
     2521 gcaatcgtgc accacaatta gtcgcacaac gtttgttgcc tgacaatcct caagatgcct
     2581 cacgtcatca caaacgccgc cttgccatca aaggcacaga aggcttagtt ttatcctatg
     2641 cccgatgttg ttatccttta cccggtgaca gtatttttgg tcatcttagt gcaggacgag
     2701 gtatcgttgt tcatcgggat gtatgccatc atttgattaa tgaattacgc aacaatcctg
     2761 ataaatgtgt tcctttgcaa tgggcaaagg aagtcatccg cgatttttct agcgaattag
     2821 ccatcgaagc tgtgaatcaa cgaggtattt tggccgaaat tacccgtgca ttagatttac
     2881 acgaatctaa tattgaaagc attaatagca aacaagacta tgatcaaggt catgccacta
     2941 ttatcattgg gattagtgtc aaaaaccgta ttcatttagc acaaatcatc aaacgtctac
     3001 gtattctaaa tggcatcgaa aaagttaccc gcgtcaaaca ctaaagcctg ttggattcat
     3061 ctgtttttca ttgcctttcg tccaaaaaac ttacaaaaaa cgtgcatttc atcacaaaaa
     3121 agctataatc ggtgccatta attatataca acttggttag taggcatatt gattgatggt
     3181 aaaacacctt tatcgttatt ttttgatgat cgtagtttgc tacttaaact tggttttaag
     3241 cacaaatacc tatgccacct cacccaatgt tgctttattt tatggggcta atccccctct
     3301 caacgaactc cgtacctttg atattgtcgt ggttgaccct gaccatgcca acattgagcc
     3361 acaaacctat aattctgaat atagtcaact atttgcctat gccagtgtag gtgaagttca
     3421 tcgtagcaaa gcttactttc agcaaattcc taaaaactgg ctaaaaagca aaaatcaggc
     3481 atgggatagt tatgtccttg accaaagttc acctgagtgg ccaaactttt ttgccgaaca
     3541 agtgattgct ccactttggg caaaaggcta tcgtggcttt tttttagata ctttagattc
     3601 ttatcaatta attagtcaaa ctcagcaaca acgccaagtc caagaacagg ggctaataaa
     3661 taccattaaa accattaaac aacgttggcc agaggctaaa ttaatcttta atcgtggttt
     3721 tgaaattttg ccacaagtcc atgatgctgc ctttatggtg gctgccgaat ctctctatca
     3781 aagttggaat gcaggcgcaa accaatatca aactgttaaa agtgaagatc gtgcatggct
     3841 gattaatcaa ttacagcaag tcaaaaacaa ctatcaattg cctattttag tgattgatta
     3901 tgtacagaat gccgagcgtg agcttgctcg acaaaccgcc agcaaaattc gtcaagaggg
     3961 ttttatacct tgggtgagta cgggggattt agacagctta ggtgtaggta atttagaagt
     4021 attacctcgt aaagtattgg taatttataa ccccaatgaa gcccccgacc tacactatca
     4081 tgatgcagtt cgatttttag gtgcgccatt ggcttatttg ggcttagtgc ctgagtttat
     4141 cccctttaat agccaacttc ctaataaaaa tttagtcgga cgttatgcgg gtattattag
     4201 ttggattaat agtgatgata ttgccaataa tagtgcttat cctcaatggt taagccaaca
     4261 aatcaaacaa ggactaccag ttgccatttt tagccgtttt ggcttttctg ccgagacagg
     4321 tttatttaat cagttgggtt tacgcagcgt tgatgttaat agcaatactc cgctacaagt
     4381 caagcacact gataaaatga tgggctttga actgcctgtg gttgcccgtg ccgaagagtt
     4441 atatggtgtg caattacaat cagcaacaca gcctttactc accttacata atcctcaaac
     4501 tcaacaaaat tatcatcctg cggccttaac cgattggggg ggatatgtcc ttgcacctta
     4561 taccgtggat gttctaccag ctaaggaagg tggcgaacgc tggttaatta atccgattag
     4621 ctttttaacc aaagccttaa aactggatga acaacgcccc attgccgatg tgactaccga
     4681 aaacggcaat cgtttattaa tggtgcatat agatggtgat ggctttatga gtattgccga
     4741 acgtgcaact cgaccattta atggccaagt attgttggat gattttttta aacgttatca
     4801 aatccccact accatgtcga ttattgaggg cgagttaggt gcagaaggtt tgtatccaca
     4861 acaatcccca gctttagaaa aaatagcacg cgatatttat gctttaccgt ggatagaatt
     4921 ggctagccac tcttattcac atcccttctt ttggagtaaa gcacaacaaa gtgccgatga
     4981 tacaggcgat tacgaaagct atcatttacc catcaaaggc tacaaatata aggttgaacg
     5041 cgaagttgct ggctcaatta attatattaa tcaaaaactt gctccaaaaa acaaacaagt
     5101 taaagtattt ttgtggacag gtaactgtgt tgctactccc gaagcattgg ccgaaaccgt
     5161 taaaacaggc gtgttaaata tgaatggtgg cgataccacc attactcgtt ctaataattc
     5221 atggacgcgt attgcaggat taggtcttaa aaaaggtaat aacttccaag tatttgcgcc
     5281 taatcaaaac gaaaacgttt ataccaattt gtggacaggc cctttttatg gttttgagcg
     5341 cgtgattgaa acctttgaat taaccgacaa accctatcgc tttaaaccca ttaatattta
     5401 ttatcactct tatttggtct ctaaaacagc aggagccaat tctttacata aaatttatca
     5461 atgggcatta aagcaaccag tattacctat ttatagctcg gaatatattc aaaaggtttt
     5521 agattttaat gatttagcga ttgcacgcag tccacaaggt tatcgtattc gcggcaataa
     5581 tcaattaaaa accttacgtt ttaatcattc taaagccatc gattttaatc atagtccgca
     5641 gttaatgggc tttaatcatc ataatcaaga gcaatatttg catttagcta acaataatct
     5701 tgatataaat atacaagaca cgccttctac acttgcctat attgaatcag ctaatgggca
     5761 aattagccaa tttcaacgct atctaaatag tgtaactttt aatatcaaaa gttcccaacc
     5821 actgcgtgtc agttttgcca atagtcaaaa atgtgtacta aaagccaatg gcaaggtact
     5881 caaaacacgt caacaaaatt ctcgtttatt gctggaggta gatgctcatg agcttagctc
     5941 gctcaggctt tcctgcactc cctgaacgtt taaggtttgc cacgacagga caaattttgg
     6001 gttttgggtt attaaccctg ctaactttta tggtgattta tcctgaacac accttacaac
     6061 gtcatttaga acgctcggca cgcacagata atgtcagtat cgcttattta tttgcttggt
     6121 taaaagctga tgcaagcgat catcatttac gcattttgtt agcacagcgt ctctttgata
     6181 aaggagagat ttctcaatct cgacaagtgc ttaatccaat ttttaaagtc aaagaattaa
     6241 gcactgaaca agaaagcaaa gcgaatattt tgttgttaga tattttagaa cgtcagttat
     6301 ggacttatca gccacaaagt gccactttta ataatgcact caagcagtat ttgcgccaat
     6361 tacgcaaaat tagttattac tcatggccgc aagctcgctt agaaaaattt gccagtaatg
     6421 cttatgcgtt tgccgattat accttggcca aagaacttta ttatcagtta atttttagtg
     6481 cctcagcggt caatccacaa tggtttgaaa agcttgctca actttatatg gtacaaggcc
     6541 aatatcgctc tgctgcgcgc agtttttttc aggctatgcc ctactgtact aatttaacgc
     6601 aacgcaaaac attttttttg tcaggcgtaa aagctttgca agcaggcaat ttagtcaatg
     6661 atgcccttgc tgcggggcaa atgtatgtgg gtgtgtttat tcatgatgcc gccactattg
     6721 aatttttagc acgtttagct ttatcggcca atcgccccga tattgctcaa tattatgttg
     6781 cgttactact caaacaacgc attaactcgc catccacagg agcaacccca tgaagtggct
     6841 gtatgcgctt ggcttgtgta ctttaagttt agccgctagt gccgaaacgc ttgatattgc
     6901 ccaagaagca ggcagtccac aagctcagtt tgatgattta cgttatcgtt taggttttga
     6961 tgtatatcta gcgaataaaa atctacctgc cgcatggcaa gttgccaaaa aagcagtatc
     7021 ggtagagcct aataatttat tttggttaga acgttttgcc caagtttctg aatggcttgg
     7081 caaacctgaa gaagctctgc ctatctggtt acgctacgca caaaaaacag gtcaacaatc
     7141 ggcttgggat aatgttaaac gtttggccat tggtttatat gacgataaag cactcctcat
     7201 ctatcaacag cgtcaagttg gtttaaaacc caatgatgcc gccgaaatag agacgcttgt
     7261 gcaaatttat gagcgtttag gtgaatctgt tgctggctta gattttttgg cacaacttcg
     7321 acacaaaagc caagccaaac gcgccctact aaccgccgaa gctaatttgg ccgaacgtgc
     7381 aggccaagac gagcgcgcga ttaatgctta ttctcaactc atcagcctat ttaaacctgt
     7441 tgaccaaaca tggttattaa gacgtgccgc attgtggtat cagcgtggca aattagtcga
     7501 agcatggcaa agtttgtctg aattagaaca tcatatgccc gatcgcgccc aagattactg
     7561 gcatacttat gctgagttat ccagattatt aaacaaaacc gatattgctc aacgcgctta
     7621 tcaaaaactt actgaggaat ttttatttgt tgacggtgat ttgattaatt atgccgccct
     7681 agaaaccgaa aaaaatccac ttaatgcggc ctttttaagc gagttatctt atcgtttata
     7741 caaacgagat aatgccgttc taagtttgtt atatgcctat caaaaagcta attacccaca
     7801 aggcatagag cgttttttga gcttattaac tacagaagaa ctgcaaaaat ttgaacaaaa
     7861 ccccttattt ttagagcaac gaggccaatt ttattggcaa caaaaacaat tagtaaaagc
     7921 acaagctgac tttgaactgg ctcttaaatt agcccccaaa caaagccgtt tattaaatgc
     7981 tttagtgggt gttttgatag agcaaaacaa acgcactgac ttaaatacgg tattacaagc
     8041 ggcacaccgc caagcgcaac gtcaagctgc tttatggcaa gtatgggcaa atgggtggtt
     8101 atttttacaa aagccacaac aggcgttgcc gtttcaatat gcttattatc aaaccaatcc
     8161 tcaagatagt ttagcaggtt taaacttagc cgaactcttg gccaatacag gtgcaactca
     8221 acaggcacaa gccttacaac aacaaatttg gcaacaacgt caacagcgca tacagcaaga
     8281 aacagacgga cgtttgcagc aattacaaca acaactgttt gctttacaac ttaatcaaca
     8341 aacaggtgat gcaagtcaat tattgttagc acaacatctt cgccatcatg cttatcagca
     8401 atttgaacat gagatttatt tagggtggtt gctaaatcat cagcataacg accgcgctca
     8461 acaattaatt gctcaacacg cgcacaaatc aactcccagt tgggctagcc taaatttagc
     8521 cttacagcaa caagaccaac gccaactaaa tcacttattg tctaatcagc ttgccaactt
     8581 gccaatttat gatcgtatag aagcggcaat gcgtgtcaat cgtgcagatt tggccgaaga
     8641 tttagccttt aacacgcaag acgattatcc taatgacgat gagctacatc gccgttatcg
     8701 tgatttattc agcgagcgtg gtcatagcgt tgatattagc cacaaacagc aagaattagg
     8761 cgcgttacaa cggcaaaata ccacagtaag ttggacaacc cccctcaaca aagattggcg
     8821 tttacaagca gatattgaac aaaatcaata tcaaagctta gaccaaagta tttttagcaa
     8881 tgatttagct agtcaacaac gcctcgcttt cacggctatg tatcatcgcc aacaacagca
     8941 aatttctgta caaattcaac agtggcaaca tgaacttggt tgggctatta aacagcaata
     9001 taaacttgat agccgtttga gcttgggctg gcagtggcaa cagcaacaaa ctagcgaaga
     9061 cagtacggca ttgttagttg caggcaaaaa aaatcgactg gcggtaaatg gtacttggaa
     9121 catatcaggc agagagtatt tgttagtcga tattgcccaa gatgattatt taactcagta
     9181 cggcgacaat ttgggttcag gccaaatttc tcaattacaa gttggtcatc gtttatttgc
     9241 cgaccaactc gatcatgtac ttaaactggg ttatagcctc ggccaattta gcactaatac
     9301 ccaaaaccta gatcagcgtt taagttctct cacggtcgca ggacaatcgc tcaacagcca
     9361 gttttacatt cctcaagact atcaacaatg gtctttaatg tgggcatttg gccaagtcaa
     9421 tccccaacaa tatcaacgcg gttggcgtgt tttgggtgaa ttggggttta atcatgccaa
     9481 taccaatggt ttaggctttg caggtcagct aggtattcaa ggttcgattt tcggtaacga
     9541 ccgtttgcta ctacaactgc aacgtagtca aagtggtcaa caaaatggcg agcaaggcca
     9601 aacttggcag ctcagctatc gtttatttta ttaaaggagc gatgcttcat gtctaaattt
     9661 tcactcagtg ctttggtcat tggtctatct ttattacaag gttgctcaac cctacacaca
     9721 acaccaaaaa tcactaaatt agatacttct gcacaatggg tattagtgcc atctattaac
     9781 aacaccgaaa ccccacaagc tggtgcacgt ttggatagca ttaccgccag tttattacga
     9841 gtgaatgggg ttaaccaatt aagcgtctat caaagtaata acaacaacga gagtctgttt
     9901 gacttggctg accgccgtaa tcaagaacaa gctctcaatg ccgtcaaaag cacaggtgca
     9961 aaatatgctg tggcggccag tgttgatgaa tggcgttaca aagttggctt ggatggagaa
    10021 cctgcggctg gtgtcacagt caatattatt gacttaagca caggtcaagt ggtgtggtca
    10081 ggtacagccg ccaaaacagg ttggagtcgt gaagcagtga gtgctattgc ccaaaaaacg
    10141 gttgatcaac tgttaaaaaa tgcgttaagc cgttaatgat ttaggtattt gaccatgttg
    10201 catttgttta ctcgtcgtca actcgctgcc gtttttgctc ccaaacaagc aggacggcta
    10261 atggttgtcg aaattgtttt gtttacctta gcaaccattg cgattggtgc atggtcaaat
    10321 cctaaagacc catttatggt gcatctgccc tttccttggt tatggttagc cccaacaatc
    10381 ttagccttgc gttatggttc attagccgct attggctcta ttggtattat ttctttgtct
    10441 tggtcggctt gggatacatt gagcaacaat actgagcttt ttcctgccca cttttttatg
    10501 ggtggcttaa ttttaagttt gcttgcaggc gagtttgccg atgtgtggat tgtgcgttta
    10561 aaacgggtac gtgaagctaa tggttactta aatgagcgtt tagagtcatt aacccgtcgt
    10621 cattatttgt taaaactgtc tcatgagcgt ttagaacaag atatgttaac taaacccatg
    10681 accttgcgtg attcattaag ccatatgcgc caagtgatga ttgaaggcgg ctttaacacc
    10741 gataaaaatc ctttaccaca agcagccgac ttaatgaatc tattggcaca aacctgtcaa
    10801 ctagaagttg ccagtttata tagtattcat aatgataata ttaacgcgac tgcggtcact
    10861 catttgggca gtgttcacag cttcaaagct gatgacccct tggtcagtta cgcacttgaa
    10921 cataaaagcc tcgctcatct acaaactgaa caattaatgg atagtcacag ccaatatgtg
    10981 gttgtcgcac cactgattgc caccaatcaa caatgttttg gtttgttaac cgtagagcgt
    11041 atgccttttt tgtcactcaa tacagaaaca ctgcaatttt tgagtgtgtt attaggctac
    11101 tatgccgaca acgtcaaagt cattcctcaa gccctgaaaa ttttgcgtga caatcctaaa
    11161 tgtccgcttg attttgcttc tgagctactc cgattagaac gtgtacaacg tgaaagtggt
    11221 ttgccatcta gtattgtcgc ttttgtgctt tcagacagtg tccatcgcca agatatttat
    11281 gccgaaatgg ttcgccaacg gcggcaaatg gatattgcgt gggatattcg tttatcagac
    11341 cgagatattc taattaccct catgccacta catggtgatg ctgccgtaac aggttattta
    11401 ttacgcacac aaaaatggct caaagatatg tttaattgca gcaaatttgc tgatgcacaa
    11461 atgactccct acgctgctct cgttaatgaa cgacctgcgg ctgaattact caatcacttg
    11521 ctagaacgtt gtttagtcaa acaaagttaa gggcgaatat catgctaagc accaatggca
    11581 aactcgtcac acttgcagtt agtttagagt tatcggctat tacactgttg tttttatcaa
    11641 gtaatagcac atggttgatt tatcaatact tttttcttca tgctttagca tccttagcca
    11701 ttacgcctgt tgcatggaca ttaatgcctg ccaactacaa acatccacgc ttatgggtga
    11761 tgtttttatt atttagctta agttttttta ttcccattat tggtttattt ggttttgcga
    11821 ttggttcttt agtggcaagt cggttgcctt attttaacaa tactcaccaa atcaaaattg
    11881 ttcacagccc cgaatatgag cttcctaagc ataacaacga aacaggttgg cgatctgctc
    11941 gcgtgcgtca gcaactcacc gacaaagaca cgcctgtaga attacgcatg aaagctttat
    12001 tatccttgca aaatatgcca gctcgtcatg cctcgagtat tttgcgcgaa gcccttagcg
    12061 atagctctga tgatttacgt ttattggctt atggcatgtt agacagccga gaaaaacata
    12121 ttactcatcg aatacagctc gctttaatca gttatcaaaa agccatatca aatcaagaac
    12181 gttatttacc tgcgcgtgag ttggccgaat tatattggga attggtctat caaaacttag
    12241 tacaaggcga tatgcgtaac tttgctttag agcaagtaca aaaatacact accgaagcac
    12301 tcaaagaaaa agtcaaagat gcaggtttat ggtcaatttc gggacgaatg tggacgattc
    12361 gtggtgatta tatccgtgca acaggctgct ttagcactgc cattaagcaa ggttttccct
    12421 tagcacgcgc cgaaccttat ttggctgaat tagctttttt acgtcaagat tatcaaacca
    12481 ccagacgtat tatgaaaaac ctcagcgaac gtggcggttc acgccctttg ttaatggcca
    12541 gcgattattg gagtaaataa catggcaatt attaaacaag ggcagtctgc tgatattggt
    12601 ttattactcg aaggcactta cccctatgtc agtggtggcg tttcgagttg ggttaaccaa
    12661 attattaaag gctttccaca atataccttt gccttatgtt ttattggtag ccgcccagaa
    12721 gattatggtg atatgcgttt tcaattgcct gataatgttg tgcatctcga aacccattat
    12781 ttgcacgaaa cacatagcaa accacctgtg ataaaacgca aaggcaaccc acaagttttt
    12841 aaagagattc gttgtttgca tgacctatat cggcatcccg aacaaatgca gcaacaagaa
    12901 acatcattac tgaacagcat aatgaatcat cgtcttgctg gccgttatgc cgaagatgat
    12961 tttttataca gcaaagaagc atgggattac attaccgaac aatatcgcgc tcactgcacc
    13021 gatccgtctt ttgtcgatta tttttggaca attcgcatca tgcacgcacc catttggcga
    13081 ctgatggctt tgaccgaaag ttttattccc gttaaagctt ttcataccat ttctacaggt
    13141 tatgcaggtt ttttgggtgc gttaatgaaa caaaaaacag gcaaaccgct aattttgtct
    13201 gaacatggga tttatactaa agaacgaaaa attgaccttt ttcaagcaga atggattcgt
    13261 gataatcgag gcacactggc taaagactcc agcgaagttg cctattttaa aaatttatgg
    13321 attcggtttt ttgaagcctt aggccgcgat tgttatcatc atgccgatag aatcattgcc
    13381 ctttatgaaa ccaaccgttt acgccaaatt aaagatggtg cgcctgctga acgcaccgaa
    13441 aatattccca acggcatcaa cctaccccat ttagaaaaag ttcggcaaaa ccgcagcgac
    13501 acaatcccta tgacggtttg tttaattggt cgtgttgtgc caatcaaaga tattaaaaca
    13561 tttattcgtg ccatgcgtac cgttgccaac caactaccac aagttgaagc gtggattgca
    13621 ggccccgaag atgaagaccc agaatatgct gccgattgcc atagtttggt taatggttta
    13681 ggtttaaacg atcatgtcaa atttttaggc tttcaaaaaa ttgatgaatt gctgcccaaa
    13741 gttggcttat tggtacttag ctccatttcg gaagctttac ccttagttat tttagagggt
    13801 tatgccgcag gcgtaccgtg tgtatcaacc gatgtcggtt catgtcggca attaatagaa
    13861 ggcttagggc cgattgataa atctattggt gaatctggca ttgtcgttgg tatcgccaac
    13921 cccaccgaat tagctgaagc cattattgcc ctattaggcg acccagaacg ttggcacaaa
    13981 gcacaagccg caggcattac ccgtgttgaa cgctattaca ctcaagaaca aatgtttgct
    14041 cgttatcgta gcgtctatga acaagcctta aacctgtcac aacaagtcac ttgcccaaga
    14101 ggaaaacgct aatggctggt attggttttg agttacgcaa actattacgc cgagatagct
    14161 tattaggcat gttacaagct tatgcttatg caggcattat cagctctggg ccttgggtat
    14221 tatccatcgt tggcatttta gtcattggtt taatgagtgc cgcgattgtt gtacccgaat
    14281 ttttgattac tcaatttcaa gtgagtgtca cttatcttgt tgctttaagt ttaattttaa
    14341 ctggctttgt tcaactggct tttacccgat ttaccgctga ccgactgttt gaaaaacgtg
    14401 ccgatattat tttacccaat tttaatggtt tattatttgg ggtaaccgca attagtggct
    14461 ttttaggcac attggccatc ctatttttat ttccacaaca aagcattatg tatcgtttat
    14521 taatgttagc gggatttgtg gtgttatgtg atatttggat tgccaccatc tttttgtcag
    14581 gtatgaaaca atataaagcc attttaaatt gttttgccgt tggctatggg gcaaccgtct
    14641 tattcgccct actattaaga aactacaaac tagaagggtt attaggtgga tttgttttag
    14701 gtcaatttat tttattaatt ggcatggtga ttttaatttt acgcaactac ccttctagct
    14761 actttattgc ctttgatttt gccaaaaaac gacagttttt ttggagttta gtttgggtag
    14821 gcttttttta taattttggt ttgtggctcg ataaatttat gttttggttt tatgagccaa
    14881 ctagccaacc tatcattggc ccattacgcg cctcattaat ttacgatatt cctgtttttt
    14941 tggcttatct ctcaatcatt cctggcatgg cggtattttt agttaaaatg gaaaccgact
    15001 ttgttgaatt ttatgataat ttttataatg ccgtgcgtga gggtggctca ttagaataca
    15061 tggaaaaaat gcgtaatgag atggtattta ctattcgtca aggtatttat cagatcatta
    15121 aaattcaagc cattaccacc ttagcggtgt ttgtggcggg tgaaattatt cttaattttt
    15181 taggtatttc tttattgtat ttaccgttgt tatttgtcaa tttaatcagt gttggtttac
    15241 aagtggtatt tttagggatt ttaaacgtat ttttttattt agataaacgc cgtatcgtgt
    15301 tattactcac cgcgctattt gtgctattta atggcatatt taccgcaatt accttatatt
    15361 taggccctaa attttatggt tacggtttag cggctgcttt attagtgtgt attatgattg
    15421 gcttccgctt tttagaccgc gtttttgaag agttagaata cagcactttt atgttgcaat
    15481 aa
//
LOCUS       DAOQMB010000014        15357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1400_length_15357_cov_16.852634, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000014 DAOQMB010000000
VERSION     DAOQMB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15357
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1400_length_15357_cov_16.852634"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..445)
                     /locus_tag="PLY05_01225"
     CDS             complement(83..445)
                     /locus_tag="PLY05_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01953.1"
                     /translation="MAVELGNHFPQRWQRAPVELRQKIFDELQDICRSLGGVINERVI
                     ASPMTARPPSVPLAPRVAPTGVAPSALEQELKKRFLQNADDLIEQHLDKVREDLRRWL
                     HGEMNQLLSDSLKKTNTP"
     gene            complement(454..876)
                     /locus_tag="PLY05_01230"
     CDS             complement(454..876)
                     /locus_tag="PLY05_01230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HNC01954.1"
                     /translation="MLQVNFYLLSTNSVESRLLTACRLVDKALQAGQRVFIQLENDTD
                     AQACDEWLWSFKAESFIPHHRLGDGKFIDAPVLLGLKTPVEADGVLLNLSTRAPQGLA
                     QFQRVLEVIANDEQLKQAGRSRWQYYRQQGFRLEKYDL"
     gene            complement(863..2347)
                     /locus_tag="PLY05_01235"
     CDS             complement(863..2347)
                     /locus_tag="PLY05_01235"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNC01955.1"
                     /translation="MDFALLSTVNPALVPADALIIAMTTQGLTATGLALDEASAGQLS
                     AALKAANFEAKLGQTVSLYRVNNISCPVIVVVGAGDSKEFNGRALQKVAAQAYKTLNS
                     KMQAAYSFLSELTVTDRAAAQVISDIARSTLEVDYRFDQFKSKKNEANNITMTLVVGE
                     ELANATGQAALSWGQAVATGQSFARDLGNLPPNICHPSYLAEQATALAKGQRKLSVDV
                     LGEKDMEKLGMGAFLAVSKGSEQEGKLIVMNYTGGKKGEKPVVLVGKGITFDTGGISI
                     KPAAGMDEMKFDMCGAASVFGVMKGILAAKLPINVVCVVAAAENMPSGRATRPSDIVT
                     TMSGQTVEILNTDAEGRLVLCDALTYVKKYDPAIVIDIATLTGACVVALGKVVSGLFS
                     PSDSLANDILQAGEQSNDRAWRMPVLPDYQELLDSPFADIGNIGGPNGGAITAACFLA
                     RFTKDYTWAHLDIAGTAWLSGAQKGATGRPVPLLMQYLQHRVTG"
     assembly_gap    2348..2447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2743..3855
                     /gene="lptF"
                     /locus_tag="PLY05_01240"
     CDS             2743..3855
                     /gene="lptF"
                     /locus_tag="PLY05_01240"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HNC01956.1"
                     /translation="MIIRRYLSGQLLITTIAVTSLLTLILMGGRVIKFFGMAAEGRLD
                     VNLLSAVLLYRLPGFLELIIPLSLFIAVLLVFGRLYIDNEMAVLSSSGISRWQLIGSL
                     VPVVLLITVTVASLSLYFTPKGNYLYEKVFSQQANRNAFDMVKVGQFQKIDSHMLYAN
                     AMSTDKSQLLDVTLYSEKQREHDTSQIMLFAKTATRQIDANTGRQTLILTDGYRYELV
                     AGQIAYNRLAFKNYQMRLRFEVQEPEITKIRAFSTPDIQQRIQQGDKIALGEWLWRCS
                     MPLLAPTGLLLAIALSKVNPRQGRYLKLLPAILLYISLVVLIGATRNKIAKGKMAEIG
                     LWSVHIFYFGLGILLLSWDDIKLRYQAWRDAKGAAV"
     gene            3852..4919
                     /gene="lptG"
                     /locus_tag="PLY05_01245"
     CDS             3852..4919
                     /gene="lptG"
                     /locus_tag="PLY05_01245"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HNC01957.1"
                     /translation="MSRLARYVGVTVLSAMAIVLVLLLGLDVVFSFLGELEDLRDNYQ
                     AAQAFTYVLMTLPLRLYDMISVSALIGGIVGLGLLANHAELTVMRAAGLSVWRIVLWV
                     MQPALLLVIGSLLIAEYVVPVSQPKAEAYKSLALGQSVSSGELWGYWQKQGNTFVQMH
                     NVSPEGKLHGISFYQFSADGVLINKQFAQQGDYQQKNQWLLNPIVEHKIDQKGQVESK
                     QQSSRLWHSELTPDFLQLVTVSPEYLAPTRLYRYADYLQGQGLVATQYFLEFWKKVLA
                     PIATLSMVLVACSFIFGPLRSVTMGLRLIFGIFAGLGFRYLQDFSGYASLVYDFSPLL
                     AAALPIALCLLGGVWALARVK"
     gene            5277..5999
                     /locus_tag="PLY05_01250"
     CDS             5277..5999
                     /locus_tag="PLY05_01250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC01958.1"
                     /translation="MLTWTSAQATDVGAKRKINEDNLLSRPDIGLWVVADGMGGHAAG
                     DVASQSVVNGLAQLKASSDLADFVDSIEDKLLLVNHQLRKHGQEALGGRTLGTTVVSL
                     FFTDTMGACIWAGDSRAYLFREGELSRISHDHSAVQEMIDAGMLTHEQAAYHPHRNVI
                     TRAVGGAENLYPEVRVFTVKAGDWYILCSDGFYNELPEEIIAANLIQENPEKSVQILM
                     EKALAKGALDNVTLIVIRVNEA"
     gene            6001..6366
                     /locus_tag="PLY05_01255"
     CDS             6001..6366
                     /locus_tag="PLY05_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01959.1"
                     /translation="MKKDYPQANARLAEVARSWAEGELSHDTWRKERRAIIKDIWLRK
                     KDLDSSQLGTLPIKTHKKHNTQTLPSLPIHVGLNHQLGSTTAVFLSEDTEVLQEDVFL
                     LAVLLFSMVLTTIFMVYML"
     gene            6662..7651
                     /locus_tag="PLY05_01260"
     CDS             6662..7651
                     /locus_tag="PLY05_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01960.1"
                     /translation="MSQYQPLVELEQRKNPIQKGTKLARRIVGGRYSLLRDIRLLLVH
                     AVELPWIVFPLKPEDDSHPELWPNSYPSYLDYLSVVAQMIVPTERKSVSYGTLAVIPH
                     DYVWPQEKRQSKWFFINGICTSPAMALLEAKEVAATFQRPIHLIHTPTAGVVRDLWNA
                     ITARSLRKDGYLSRPAFNIVKNALLSHEKVVLTTYSQGTIVASYIVRKVLKDPVLRLH
                     AHKLEVYCIGGAADSLHIDHALSAEHGHSVPYVEHFANGKDFFARVGVLAHYHDTSGA
                     IFVIPDKKGHMLNSHYIKGIGRGDYCDQRSRLFKYAYGGEAGAHDYIPTDRRR"
     gene            complement(7710..8675)
                     /locus_tag="PLY05_01265"
     CDS             complement(7710..8675)
                     /locus_tag="PLY05_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC01961.1"
                     /translation="MSFNEILNTVADDFAAVDHFITHHLDSRVPLIMQVSQYLVDGGG
                     KRLRPLVCLLSARATGYTEGNAHVLAAAIIEMLHTATLIHDDVVDESDLRRGRATVNS
                     AWNNATAVLVGDFLISRAFQLIVSLKSQAISEIMSDSTCIVAEGEVLQLINQRDPNTT
                     EQRYMDVIYSKTAQLFVAATETGAELGNPVYRPAMKKYAVHFGAAFQIIDDIMDYTSS
                     AEEMGKNVGDDLAEGKPTLPLIQAIKTAPTAQAQLIKDAIKTGGLEHLNEILRVVKES
                     GALDYCMQRAQEESQLAIDALAEIPASKYKDALIALAKLALKRTT"
     gene            8917..9228
                     /gene="rplU"
                     /locus_tag="PLY05_01270"
     CDS             8917..9228
                     /gene="rplU"
                     /locus_tag="PLY05_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003660674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNC01962.1"
                     /translation="MYAVIKSGGKQHRVVEGEKLKVELLNAEPGQSITLEDILMVVNG
                     DNIQIGTPVVAGAKVTAEVLGHGRHEKVRIVKMRRRKHYRKQQGHRQWFTELKITAIS
                     A"
     gene            9244..9504
                     /gene="rpmA"
                     /locus_tag="PLY05_01275"
     CDS             9244..9504
                     /gene="rpmA"
                     /locus_tag="PLY05_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNC01963.1"
                     /translation="MAHKKAGGSTRNGRDSQAKRLGVKVYGSQQISAGNIIVRQRGTQ
                     FWPGENVGIGRDHTLFAKADGVLKFVTKGPFNRQYAVVEVAA"
     gene            9693..10904
                     /gene="obgE"
                     /locus_tag="PLY05_01280"
     CDS             9693..10904
                     /gene="obgE"
                     /locus_tag="PLY05_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNC01964.1"
                     /translation="MRFVDEAIIQVEAGHGGNGCVSFRREKFIPFGGPDGGDGGRGGS
                     IWIEADPDANTLVDYRYTRKFRADRGQGGRGANCSGKSGADVVLKVPVGTTIVDEDTN
                     EVLGDLVEANQKLLVAKGGRGGLGNIHFKSSTNRSPQKATSGFAGEARQLRLELKVLA
                     DVGLLGLPNAGKSTFIRAVSAAKPKVADYPFTTLVPNLGVVDVDRHRSFVMADIPGLI
                     EGAAEGAGLGIRFLKHLARTRFLLHLVDIAPVDGSDPVENITAISLELEKFSPALAAL
                     PRWLVLNKIDLALPEEVELIEQDILERLDWQGPVFKTSGLTRDGAMQVCYKLMEAIEE
                     QRQLEAENPEFAEAEAEKRRLLEEETRQQIQNLRAQQRAQYLANKEAGFEDDDDWDDD
                     DDGDMEVIYQR"
     gene            10913..12037
                     /gene="proB"
                     /locus_tag="PLY05_01285"
     CDS             10913..12037
                     /gene="proB"
                     /locus_tag="PLY05_01285"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004668826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNC01965.1"
                     /translation="MTNNRHKLQQARRWVVKIGSALLTANGQGLDQQAMRSWVEQMVA
                     LAQQGIELILVSSGAVAEGMVRMGISHRPDSLHDLQACAAIGQMGLVQAYESHFREFN
                     CKTAQILLTHEDLSDRQRYLNARSTLTTLINWRVIPVINENDTVATDEIRFGDNDTLA
                     ALVANLVDAEALIILTDQEGMFDRDPRHDATATLLPEVRAMDESLFAMAGGGGVLGRG
                     GMITKVRAARFAARSGCATVIASGRSPNILQRLAAGESLGTLLLPDDDRLTARKQWLA
                     AHLQTAGRVVLDKGAVRALIEQGRSLLPVGVKAVEGLFLRGEAVACVDEAGKQLAVGL
                     VNYNAEEARQIIGLSSLQIADVLGYMSDEELMHRDNIAIT"
     gene            complement(12193..12269)
                     /locus_tag="PLY05_01290"
     tRNA            complement(12193..12269)
                     /locus_tag="PLY05_01290"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12233..12235),aa:Arg,seq:acg)
     gene            complement(12363..12452)
                     /locus_tag="PLY05_01295"
     tRNA            complement(12363..12452)
                     /locus_tag="PLY05_01295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12416..12418),aa:Ser,seq:gct)
     gene            12824..14089
                     /locus_tag="PLY05_01300"
     CDS             12824..14089
                     /locus_tag="PLY05_01300"
                     /inference="COORDINATES: protein motif:HMM:NF021589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNC01966.1"
                     /translation="MERKNILGVSLLGLTCLVGAQTASAEIALQPQAVPFGAFVLVPT
                     LNTKTVFDDNIYSLSSNEVSSFSQVVNPNFAFVAQDRANVYKLVYNLNAAGYANDSDD
                     SYNDHKIDLNAHIEPTARLRYDAGLAYGMLHDDRGQGASSGLSLFRIRQDGNGDGVID
                     EGIGEVDKYNLATVRGGVEYGAKTARGLLVANADLNQKRYSRNSSALGRDNDTFNVLL
                     GVRARLMPKTTFLVDYEISDTNYKTDPKNGGTADTKDNRILAGITWENSIQTTGKLRL
                     GQGKRKVDGGKDIDKFTWDLGMVWKPLPLDTINITGGAKATDASAPYAATENTNYNLS
                     WTHQWLDRVNTTVNFGMSKDDYTVAPNAPAGTKARSDDTTNYGVALNYQMRRWLVWSV
                     GVNSSDKDSNVKEFSNTRNVMSIGAQVSL"
     gene            14107..14739
                     /locus_tag="PLY05_01305"
     CDS             14107..14739
                     /locus_tag="PLY05_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HNC01967.1"
                     /translation="MQNLRLWLVLLSMLAWTPVHAGLLDRFFGLPTDKENNSAVVTPP
                     PLIVDQPIVVSSNVSDYRLGAGDKLNITVFGEKELSMEIRLSDAGSFLYPFLGEVQAK
                     NKTISELQFLLTSSLKDGYLVDPKVYVGILEYRPFFINGEVGKPGGFAYQPGLTVRKA
                     ISLAGGLTPRASLTKIYVVHENDPTGTPRLTTLNAVIQPGDTITIEQSFF"
     gene            14754..>15357
                     /locus_tag="PLY05_01310"
     CDS             14754..>15357
                     /locus_tag="PLY05_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HNC01968.1"
                     /translation="MERFDNTLPVQQEDAIDLRHYWRVLMRFKWGILGLAIAVTLAAI
                     FFVSAMRPVYKASTTLLIEQKQGKTLTSLDDVYGADVTGKEYLQTQFEILKSRELAAR
                     VVKELNIAQHPDYLIRNQVLQEDEKSWWQLIDWQQYLPVGHEKNPIPTEDEQFNKLVD
                     KFMTNVSIEPIRQTQLVKITFQSYDRVLTARVSNAMAKVYI"
BASE COUNT         4338 a         2776 c         3444 g         4699 t
ORIGIN      
        1 gctgactcat caacagtatc agccgcacac ggatttagcc aaacccaagt ggctaaaaga
       61 gcaaaacagg aggttttttg tctcatggag tgttagtttt ttttaggcta tcactgagta
      121 attgattcat ttcaccatgc aaccaacgac gtaaatcttc acgaacttta tctaaatgct
      181 gctcgatcag atcatcggca ttttgtaaaa agcgtttttt cagttcttgt tcaagggcag
      241 aaggggcaac gcctgtagga gcaacacgtg gtgctaatgg cacagaaggc ggacgagcag
      301 tcatcgggct ggcaatcaca cgttcattaa taacgccgcc taaagaacga caaatatctt
      361 gtagctcatc aaaaatcttt tgtcttaact caacaggagc tcgttgccaa cgttgaggaa
      421 aatgattacc taattcaaca gccataagcg gactcacaaa tcgtattttt ctaatctaaa
      481 gccttgttgt cgataatatt gccagcgaga acgtcctgct tgtttaagtt gttcatcatt
      541 tgcaatcact tcaagcactc gttgaaattg tgccaaacct tgaggcgcac gagtacttaa
      601 attcagtaac acaccatctg cttcaacggg tgtctttagt cccaataata caggcgcgtc
      661 aataaatttc ccatcaccta gacgatgatg aggaataaaa gattctgctt taaacgacca
      721 tagccattca tcacaagctt gggcatcggt gtcattctct aattgaataa aaacccgttg
      781 cccagcttgc agagccttat ctaccaaacg gcacgccgtt agcagacgcg actctacact
      841 atttgtcgat aataagtaaa aattaacctg taacacgatg ctgcaaatat tgcattaaca
      901 aaggaacagg acggccagtc gcgccttttt gtgcgccaga caaccaagct gtgccagcaa
      961 tatccaaatg tgcccatgta tagtctttgg taaatcttgc taaaaaacag gcagcggtaa
     1021 ttgcgccacc gttaggccca ccaatattac caatatccgc aaagggggag tctaataatt
     1081 cttggtaatc aggcaacaca ggcatacgcc atgcacggtc attactttgt tcgccagctt
     1141 gtaaaatgtc attggctaaa ctgtcacttg ggctaaataa gccagacacg actttaccca
     1201 aagccaccac acaagcacct gttaacgtgg caatatcaat cacaatcgca ggatcatatt
     1261 ttttaacgta ggtaagggca tcacacaaca ccaaacgacc ttcggcatcg gtatttaaaa
     1321 tttcaacagt ttggccagac atcgtggtaa caatatcact aggacgcgtc gcacgaccag
     1381 agggcatgtt ttcggcagca gcgaccacac aaaccacgtt aatcggtaat ttagcggcta
     1441 aaatgccttt cattacacca aaaaccgaag ctgcaccaca catatcaaac ttcatttcat
     1501 ccatgccagc cgcaggttta atcgaaatgc cacctgtatc aaaggtaatg cctttgccaa
     1561 ctaataccac aggtttttcg ccttttttgc cacctgtgta attcatgaca atcaatttgc
     1621 cttcttgttc tgaaccttta gacaccgcta aaaatgcgcc catgcctaat ttttccatgt
     1681 ctttttcgcc taatacgtca acacttaatt tacgttggcc tttggcgagg gcagtagctt
     1741 gttcggctaa atagctagga tgacaaatat tgggtggcaa gttacctaaa tcgcgggcaa
     1801 aactttggcc agttgccacg gcttgtcccc agcttagtgc cgcttggcca gttgcgttgg
     1861 ctaattcttc accgacaact aaggtcatgg tgatattgtt agcttcattt tttttgcttt
     1921 taaattggtc aaaacgataa tcgacctcaa gcgtgctacg agcaatgtca ctaatcactt
     1981 gtgcggctgc acgatcggtc acggttaatt cgcttaaaaa gctataagca gcttgcattt
     2041 tgctatttaa ggttttgtaa gcttgagccg ctactttttg taaagcacga ccgttaaact
     2101 ctttactgtc accagcaccc accaccacaa tcacagggca gctaatatta tttacacgat
     2161 aaagagagac cgtttgcccc agttttgcct caaaattagc cgcttttaag gccgctgata
     2221 attgacctgc actggcctca tcaagtgcta aacctgttgc tgttaaacct tgtgtggtca
     2281 tggcaataat taacgcatca gccggcacaa gggcagggtt aacggtagat aacaacgcaa
     2341 aatccatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacg catcagccgg
     2461 cacaagggca gggttaacgg tagataacaa cgcaaaatcc attacaacct ctctcacata
     2521 aatttttgtt gatgagtgat gataaagaac catccaaaga taagctcatc tagtgttaca
     2581 atatggccta gtttaccgat tcatgattgt tttaacagta tacggcttac gcatttcagc
     2641 gaaattcatc tgttttgtga atataaaatc actaaaaatg attggaatat aaacaaataa
     2701 gcaacaaccc tgtaagtcac accaagaaaa ggcaaagtta ttgtgattat tcgtcgctat
     2761 ttgagtggtc aattgttgat aacaaccatc gctgtgacca gtttgttgac actcatttta
     2821 atgggcggac gtgttattaa gttttttggc atggcggcag aaggtcgttt agatgtcaat
     2881 ttattatccg cagtgttgtt ataccgttta cctggttttc ttgagctgat tatccctttg
     2941 tcgctgttta ttgctgtgtt attagtcttt ggccgtttat atattgataa cgaaatggcg
     3001 gtgttaagca gtagtggcat tagtcgttgg cagctaattg gtagcctagt gcctgttgtt
     3061 ttattaatta ctgtcactgt tgccagttta agcttatatt ttacccctaa aggcaattat
     3121 ttatacgaaa aagtctttag ccaacaagcc aatcgtaatg cgtttgatat ggtgaaggtg
     3181 ggtcagtttc agaaaattga tagccatatg ttatatgcta acgccatgtc aacagacaaa
     3241 agccaattat tagatgtgac tttatatagt gaaaaacaac gagaacatga tacaagccaa
     3301 atcatgttgt ttgccaaaac agccacacga caaatagacg ccaatacagg acgacaaacc
     3361 ttaattttaa ccgatggtta tcgttatgaa ttagtggcag gtcaaattgc ctataaccgt
     3421 ttagcattta aaaactatca gatgcgttta cgctttgaag ttcaagagcc agaaatcacc
     3481 aaaatccgtg catttagcac gcccgacatt caacaacgta ttcaacaagg cgacaaaatt
     3541 gctttgggtg agtggttgtg gcgttgttct atgcccttat tagccccaac ggggttgttg
     3601 ttggctattg cactatcaaa agtcaatcct cgtcaaggtc gttatcttaa attacttcct
     3661 gccatcttgt tgtatatcag cctagtagta ttaattggtg caacacgcaa caaaattgcc
     3721 aaaggtaaaa tggccgaaat aggtttgtgg tctgtgcata tcttttattt tggcctaggc
     3781 attttattgc tgtcgtggga cgacataaaa cttcgctatc aggcatggcg agatgctaaa
     3841 ggagcagccg tatgagccgt ttagcacgtt atgtcggagt cactgtttta tcagcaatgg
     3901 ccatcgtttt agtgctgtta ttgggcttag atgttgtttt ctcattctta ggggaattag
     3961 aagatttacg cgataattat caagcagcac aagcttttac ttatgtgtta atgaccttgc
     4021 cattacgctt gtatgacatg atttcggttt ctgcattaat tggcggtatt gttggcttgg
     4081 gtttattggc caaccatgct gagttaacag tcatgcgtgc tgcaggtttg agcgtgtggc
     4141 ggattgtgtt gtgggtgatg cagccagcgc tgttattggt gattggtagc ttattaattg
     4201 ctgagtatgt agtacctgtc tctcagccca aagccgaggc ttataaatca cttgctttag
     4261 gtcaatcggt cagttcaggc gaattatggg gatattggca aaaacaaggc aatacctttg
     4321 tgcaaatgca caatgtcagt cctgagggta aactacatgg cataagcttt tatcagtttt
     4381 ctgccgatgg tgtgcttatt aataaacagt ttgctcaaca aggtgactat caacaaaaga
     4441 atcaatggtt attaaaccca atagtagaac ataaaattga tcaaaaaggc caagttgaaa
     4501 gcaaacagca gtctagtcgg ctatggcaca gcgagttaac gccagatttt ttacaattag
     4561 tcaccgtttc ccctgagtat ttagcaccta cacgcttata tcgttatgcc gattatttgc
     4621 aaggacaagg tttggttgct acacaatact ttttagagtt ttggaaaaag gtacttgccc
     4681 caattgcaac cttatccatg gttttagttg cttgttcgtt tatttttggc ccattacgtt
     4741 cggtcacgat gggattgcgg ctgatttttg ggatttttgc gggcttgggc tttagatatt
     4801 tacaagattt ttcgggttat gcgagtttgg tttatgattt ttcgccactc ttagctgctg
     4861 ctttgccaat tgcactttgt ttattgggag gagtttgggc tttagccaga gtgaaataaa
     4921 aggtttatct aggttgtgat caagtaaaaa cggctgaacc atcacaattt tagtaggaaa
     4981 ttttgttgct taacttgaac aagtgttgac agtatcattt ttttgcaaca ttttatcaaa
     5041 cagaaattag gacttatgta ttttactgaa gttagcctta gtaaactaag cactagaaat
     5101 ggttaattta taagcaaatg aacggtaaag cttatggttt taacttggct atctctatcc
     5161 taagaggata aatattttaa gcctgctaaa ctgataaata ataatagaag atatcgtcaa
     5221 gagaataacc tgctctaagc caatatcttc ttcaataaat aaaagagaat acgattttgt
     5281 tgacttggac ttctgcacag gccaccgatg taggtgcgaa aagaaaaata aacgaagata
     5341 acttattgtc tcggcctgat attggcttat gggttgtcgc tgatggcatg ggaggacacg
     5401 ctgcgggtga tgtagcgagt cagtccgtgg ttaatggatt agcacaattg aaggcctcat
     5461 ctgatcttgc cgactttgtt gattcaatag aagataaact attgttagtc aatcatcaat
     5521 tacgcaagca tggccaagaa gccctaggtg ggcgaacttt aggaacaact gttgttagtc
     5581 tattttttac cgataccatg ggggcttgta tctgggctgg cgattctaga gcctatttat
     5641 ttagagaagg agagttatct cgtatttctc atgatcattc agccgtacaa gaaatgattg
     5701 atgcaggtat gcttacccat gagcaagcgg cgtatcatcc acatcgtaat gtcattactc
     5761 gtgccgtcgg tggtgctgaa aatttatatc cagaagtgcg cgtgttcaca gttaaagcgg
     5821 gtgattggta tatactgtgc agtgacggct tttataacga gttacccgaa gagataatcg
     5881 ccgccaattt aatccaagaa aaccccgaaa agtcagtgca aattttaatg gaaaaagcct
     5941 tagccaaagg agctttagat aatgtgacac tgatcgtgat tcgagtaaat gaggcctaat
     6001 atgaaaaaag attaccctca agccaatgcc agattagccg aagtggctag aagttgggca
     6061 gaaggtgagc ttagccatga tacttggcgc aaagaaagac gcgcaattat taaagatatt
     6121 tggttgcgta aaaaagattt agatagtagt caattaggaa cgttacctat caaaacacac
     6181 aaaaaacata atacgcaaac tttgccaagt ctgcccattc acgtgggtct caatcatcag
     6241 cttggatcaa caacggctgt atttttatct gaagatacag aagtgttgca agaagatgta
     6301 ttcttattgg cagtgttatt attttctatg gtgttaacaa cgatatttat ggtttatatg
     6361 ctttaagcag ctacagtatt gacagttata agcttggttg gttagcttaa tttgtttgta
     6421 atttcttcat gtttgatacc ataagtgaga tgtaagtcac attatggtgt ttttcttttt
     6481 gttagcttgt tcaacaagat tagctaagtc acttactcac tttgatttat tatcgtgtgt
     6541 aaatcaaaaa aatatcaaaa tattcactta tttctttgtt gtgatgccaa gattaaatta
     6601 atgtggttat agaaataagc aaagtagcga ttgatattgt gtagtctaaa gagagtcata
     6661 aatgagtcaa tatcagcctt tagttgagtt agaacaacgc aaaaatccaa ttcaaaaggg
     6721 aaccaaatta gcacgacgta tagttggtgg tcgttactct ttattacgcg atattcgttt
     6781 gttgttagtt catgctgttg aactgccttg gattgttttt cctttaaaac ctgaagatga
     6841 ttctcatcca gaattatggc caaatagtta tcctagttat ttagattatt tgtctgttgt
     6901 tgcgcagatg attgttccta ctgagcgtaa atctgtttct tatggtacgt tggcggttat
     6961 tccccatgat tatgtatggc ctcaagaaaa acgtcaaagt aaatggtttt ttattaatgg
     7021 tatttgtacc agcccagcga tggcattgtt agaggccaaa gaggttgctg ccacctttca
     7081 acggccaata catttaattc ataccccaac ggcaggcgtt gtccgcgatt tatggaatgc
     7141 cattacggca cgtagtttac gcaaagatgg ttatttatct cgcccagcat ttaatattgt
     7201 taaaaatgcg ttgttaagcc atgagaaagt ggtgttaacg acgtattctc agggaacgat
     7261 tgtggcgagt tatatcgtcc gcaaagtgct aaaagatccc gttttacgtt tacatgcgca
     7321 taagttagaa gtgtattgta ttggcggagc agccgatagt ttgcatattg atcatgcttt
     7381 aagtgcagag catggccata gcgtgcctta tgtagaacat tttgctaatg gtaaagattt
     7441 ttttgcacga gttggtgtct tggcacatta ccatgacaca tcaggtgcaa tttttgtgat
     7501 tcctgataaa aaaggacata tgttaaatag tcattatatt aaaggcattg gacgtggtga
     7561 ttattgcgac caacgttctc gtctctttaa gtacgcatat ggtggcgaag ctggggcgca
     7621 tgattatatc cccaccgatc gacgccgtta aaaaaagccc ctgatggggc tttttattgg
     7681 gttgaaggtt ggcaataatt attttaatgc taagttgttc tttttaaagc taatttagcc
     7741 aaagcaatta aagcatcttt atattttgat gcaggaattt cagctaaggc atcaatcgcc
     7801 aattgtgact cttcctgtgc gcgttgcatg caataatcaa gcgcaccaga ctctttaaca
     7861 accctcaaaa tttcgtttag gtgttccaaa ccccctgttt taatggcatc tttaattaat
     7921 tgtgcctgag ctgttggtgc agttttaatg gcttgaatca acggtagggt aggtttaccc
     7981 tcggctaaat catcacccac atttttaccc atttcttcag cactgctagt gtaatccata
     8041 atgtcatcaa taatttgaaa ggcagcacca aaatgcactg catatttttt catcgctgga
     8101 cgataaacag gattaccaag ctctgcacct gtttctgtgg ctgccacaaa caattgtgct
     8161 gttttgctat aaatcacatc catataacgt tgttcggtgg tattaggatc acgttgatta
     8221 atgagctgta atacttcacc ttcagcaaca atacaggtac tatcagacat aatttctgaa
     8281 atggcttggc tttttaagct gacaattaat tggaaagcac gtgaaattaa aaagtcaccc
     8341 actaaaacgg cggtggcatt attccatgct gagttcacag tggctcggcc acgacgtaaa
     8401 tcagattcat ccaccacatc atcgtggatt agggtagctg tatgtaacat ctcaataatg
     8461 gcagctgcta acacatgagc attgccttcg gtgtaacctg ttgctcgtgc tgataataaa
     8521 catactaatg gtcttaaacg tttgcccccg ccatccacta aatactgact gacctgcata
     8581 attaatggaa cgcgtgaatc taaatgatgg gtaataaagt gatcaacagc tgcaaaatcg
     8641 tcagcaaccg tatttaaaat ctcattaaag ctcatagatg aacccaatta ttaacaaaaa
     8701 gatggtggtg tcattgttgt ccttatcatg ctaatgctaa cagtagcttg tgtttactac
     8761 cacaccttaa cgaataaatc ataaaaaaac gctttttctt taggctttgt taagagatgc
     8821 ttgcactcag tcaacatttt gcatacaatc gcgcaccttg cgaatttatc actgtgggct
     8881 tagaagaagc tccgcagatt tacggagtcg gcgactatgt acgcggttat taaaagtggc
     8941 ggcaaacaac atcgcgttgt tgaaggcgaa aagctcaaag ttgagttatt aaacgcagag
     9001 ccaggccaaa gcattactct tgaagatatt ttaatggtag tgaatggcga caacattcaa
     9061 attggtactc cagtggttgc tggagctaaa gttacagctg aagtgcttgg tcatggtcgt
     9121 catgagaaag tacgcattgt taaaatgcgt cgccgtaagc actatcgtaa acaacaaggt
     9181 caccgtcaat ggtttaccga gttgaaaatc acagctatta gtgcttaatg ggggatattc
     9241 gtcatggcac acaagaaggc gggtggttct acccgtaacg gccgcgactc acaggccaaa
     9301 cgtttaggtg taaaagtgta cggtagccaa caaatttctg ctggcaatat tatcgttcgt
     9361 caacgtggca cacaattctg gccaggtgaa aatgtaggca ttggtcgtga ccatacctta
     9421 tttgctaaag ccgatggtgt tttaaagttt gtaactaaag gtcctttcaa ccgtcaatat
     9481 gcggttgttg aagtggctgc ttaatcagtt caaactaaaa aagccccgat tatcggggct
     9541 ttttgctttg tggttagagt ttttttaaac aggacttacg catttcactg aaattagcgc
     9601 gtttgtgagc atgaggtcgt tgaaggcgat tgaaaagtga gcaaataagc aatagccgcg
     9661 taagtcctaa aaaggtttag ggcgaagaca tcatgcgttt tgttgatgaa gcaattatcc
     9721 aagttgaggc gggtcacgga ggtaatggct gtgtgagttt tcgccgagaa aagtttattc
     9781 cctttggtgg gcctgacggc ggtgatggcg gtcgtggcgg tagtatttgg attgaggctg
     9841 atcctgatgc taatacttta gtggattatc gttatacccg taagtttcgt gcggatcgtg
     9901 gtcaaggtgg tcgtggtgca aattgtagtg gtaaaagcgg cgcggatgtt gtgttaaaag
     9961 tgcctgttgg tacaacgatt gttgatgaag ataccaatga ggtgctaggc gacttagtag
    10021 aagcaaacca aaaattgttg gtggctaaag gtggtcgtgg tggcttgggt aatattcatt
    10081 ttaaaagctc cactaatcgt tcgccacaaa aagcaacatc aggttttgct ggcgaagctc
    10141 gtcaattacg tttggaatta aaagtccttg ctgatgttgg tttgttggga ttgccaaacg
    10201 caggtaaatc cacttttatt cgtgcggtgt cagcagccaa gcctaaagtg gcagattatc
    10261 cttttacgac attagtgcca aatttagggg tggttgatgt cgatcgtcat cgctcttttg
    10321 tgatggctga tatccctggt ttaattgaag gggcagcaga aggagcaggg ttaggtattc
    10381 gtttccttaa gcatctcgca cgtacccgat ttttattgca tttggttgat attgcgccag
    10441 ttgatggtag tgatcctgtt gaaaatatta cggcaattag cttagagtta gagaaatttt
    10501 ctcctgcatt ggctgctttg cctcgttggc tagtattaaa taaaattgat ttggccttac
    10561 ctgaagaagt tgaattaatt gagcaagata ttcttgagcg tttggattgg caagggcctg
    10621 tatttaagac atctggttta acccgtgatg gtgcaatgca ggtttgttat aaattgatgg
    10681 aagctattga agaacaacgt caattagaag cagaaaatcc agagtttgcc gaagcagagg
    10741 ccgaaaaacg tcgtttattg gaagaagaaa cgcgtcaaca aattcaaaat ctacgtgcac
    10801 agcaacgtgc gcaatatttg gctaataaag aagctggttt tgaagatgac gatgattggg
    10861 atgacgacga tgatggagac atggaagtta tttaccagcg ttgagtaaga aaatgacaaa
    10921 taatcgccat aaattgcagc aagcccgtcg ttgggtagtc aaaattggtt cggctttatt
    10981 aacggcgaat ggtcaaggtt tggatcaaca agccatgcgc tcttgggttg agcaaatggt
    11041 tgctttagcg caacaaggga tagagcttat tttagtctct tcgggtgctg ttgctgaagg
    11101 tatggtcaga atggggatta gccatcgtcc agattccttg cacgacttgc aagcgtgtgc
    11161 tgcgattggt caaatgggct tggttcaggc ttatgagtca cattttcgag agtttaactg
    11221 taaaacagcg caaatcttac tgacacatga agatttatcg gatcggcaac gttatttaaa
    11281 tgcgcgttcg actttaacta cgctaattaa ttggcgagtt attcctgtta ttaatgaaaa
    11341 tgatacagtt gccacggatg aaattcgctt tggtgataac gatactttag cggcattagt
    11401 ggctaatttg gtcgatgccg aagcgttgat tattcttacc gatcaagaag gtatgtttga
    11461 tcgtgaccca cgccatgatg caaccgcaac tttattgcct gaagtacgag ccatggatga
    11521 aagtctattt gcaatggctg gtggtggtgg tgtgttaggg cgtggcggca tgattactaa
    11581 agttcgagcg gcgcgttttg cggctcgttc ggggtgtgca acagtgattg ccagtggccg
    11641 tagtcctaat attttacaac gtttggcagc aggggagtca ttgggaactt tgctgttgcc
    11701 tgatgatgat cgtttgacgg cgcgtaaaca atggttggca gcacatttac aaacggctgg
    11761 acgagttgtg ctcgataaag gtgcggtacg cgccttaata gagcagggta gaagtttatt
    11821 gccagttggt gttaaggctg tcgaagggct gtttttacgt ggagaggcgg tagcttgtgt
    11881 cgatgaggct ggtaaacagt tggcagttgg tttggtgaat tataatgctg aagaagcaag
    11941 acagattatt ggcttgtctt cattacaaat agcggatgtg ttgggttata tgagtgatga
    12001 agagttaatg catcgggata atattgcaat tacataaact tgagggtggt taatctgccc
    12061 ttgtttttta tggttgtata ccaattcgtt tctgaataga gggtatcggt gttttgattc
    12121 tttgttgtag cttgtatttt gtgatgaatg ccaatgattg ctggcataaa atttgagtga
    12181 tttaatttta ggtggcgcgc ccggagagat tcgaactccc gacccccaag ttcgtagcct
    12241 ggtgctctat ccaactgagc tacgggcgcg ttgaggtggc gtattatatg ccactttttt
    12301 ttgatgtcaa catttatttt gaatttaaat cgcgtttgcg aaatgttaca aaaaaaatgt
    12361 aatggcggtg agagagggat tcgaaccctc gatacggtta cccgtatgcg tccttagcag
    12421 ggacgtggtt tcagccactc acccatctca ccgcacaggg cgcgaatcat agcattaata
    12481 taaaaaaatg aaagatgttt tttaatgtgt ttgcttaata aatgtgcgtt tgttttgtga
    12541 tcggtgtcgc attcggacga aaaggttaaa atgtccgaca atggtttgag gattgaggag
    12601 aaaatgtgct ataaaggcac gcagtttttt ggcgtatcaa ttggtgtttg tttaaaactt
    12661 gatcagcaat tgagttttca gggttaccac attatgggtg acttaaagtg agacctagtg
    12721 ctaaaattgg aagtataaat taccaagttg tatttgctaa ggtttgttag tcaatttttg
    12781 gtattgatga ctcgttgttt gattacctat tcgagggaaa aatatggaac gcaagaatat
    12841 tttaggtgtg tccttgttgg gattgacctg tttggtaggg gcgcaaaccg ctagcgcaga
    12901 aattgcactg caaccacaag cagttccttt tggagctttt gtacttgtgc caacattaaa
    12961 tacaaagacg gtatttgatg ataatattta ctctttaagc tctaatgagg tgagttcgtt
    13021 ctctcaggtt gtgaatccga attttgcttt tgttgcgcaa gatcgtgcga atgtttataa
    13081 gttggtttat aacttgaatg cagctggtta tgccaatgac agtgatgata gctataatga
    13141 tcataaaatt gatttaaatg ctcatataga gcctacagct cgtttacgct acgatgcggg
    13201 tttagcgtat ggtatgttgc atgatgatcg tggacaagga gctagttctg gccttagttt
    13261 atttagaatt cgtcaagatg gtaatggcga tggtgtcatt gatgagggta ttggtgaagt
    13321 agataagtat aacttagcga ctgtgcgtgg tggtgtagag tatggtgcta aaacagcccg
    13381 tggtttgtta gtagctaatg ctgatttgaa tcaaaagcgt tattcacgta attcatcggc
    13441 attaggtcgt gataatgata catttaatgt gttgttgggt gtgcgtgcgc gtttaatgcc
    13501 taaaacaacg tttttagtag attacgagat ttctgataca aattataaaa cagatcccaa
    13561 aaatggtggt acggctgata ccaaagataa tcgtatcttg gcaggtatta cttgggaaaa
    13621 tagtattcaa accacaggca aattgcgttt aggtcaaggt aagcgtaagg ttgatggtgg
    13681 caaagatatt gataaattta cttgggattt aggaatggtt tggaagccgt taccattaga
    13741 tacgattaat attacgggtg gtgctaaagc aacagatgct tccgctccat acgcagcgac
    13801 agaaaacact aattataatt tgtcttggac tcatcaatgg ttggatcgtg taaatactac
    13861 ggttaatttt ggtatgtcta aagatgatta tactgttgca ccaaatgcgc ctgcagggac
    13921 aaaggctcgc tctgatgaca caactaacta tggtgtggcg ttgaattatc aaatgcgccg
    13981 ttggttggtg tggagtgttg gtgttaatag ttctgacaaa gactctaatg tcaaagagtt
    14041 tagtaatact cgtaatgtaa tgtcaattgg tgctcaagtt agcttataat attgggcgta
    14101 tcatttatgc aaaacctacg tctttggttg gtgttgttat ctatgcttgc atggactcct
    14161 gtccatgcag gtttattgga tagattcttc ggtttgccaa ctgataaaga aaataactcc
    14221 gctgttgtta cacctcctcc tttaattgtt gatcagccga ttgttgttag tagtaatgtc
    14281 tctgattatc gtctaggggc tggtgataaa ttaaatatta cagtttttgg tgaaaaagaa
    14341 ctgagtatgg aaatccgttt aagtgatgca ggtagcttct tgtatccttt tttgggtgag
    14401 gttcaagcta aaaataaaac aattagtgag ttgcagtttt tattaacttc aagtttaaaa
    14461 gatggttatt tggttgatcc taaagtttat gtgggtatat tagaatatcg tccatttttt
    14521 ataaatggcg aagtaggtaa acctggtggt tttgcttatc agccaggctt aactgtgcgt
    14581 aaagcgattt cattagcagg tggtctgaca ccacgtgcat cattgactaa aatctatgtg
    14641 gttcatgaaa atgatccgac aggaaccccg cggttaacaa ccttaaatgc agtcattcag
    14701 cctggtgata ctattaccat tgagcaaagt ttcttctaat ttgggtacta acaatggaac
    14761 gatttgacaa tactctgcct gttcagcagg aagatgctat tgatttacgt cattattggc
    14821 gtgtattaat gcgttttaaa tggggcattt taggcttagc tattgcagtg acactagcag
    14881 ctattttttt tgtttcagca atgcggcctg tttataaagc atccactaca ttgttaattg
    14941 aacaaaaaca aggaaagact ttaacctctt tagacgatgt ttatggtgct gatgttacag
    15001 gtaaagagta tttacaaaca caatttgaaa ttttgaaatc acgagagtta gctgcacgag
    15061 ttgtaaaaga attaaatata gcgcaacatc ctgattattt aattcgcaat caagttttgc
    15121 aagaggacga aaaatcttgg tggcagttaa tagattggca gcaatattta ccagttggtc
    15181 atgagaaaaa tcctattcca actgaagatg agcaatttaa taagcttgtt gataagttta
    15241 tgactaatgt ttcaatagag ccgatccgac aaacgcaatt agttaaaatt acgtttcagt
    15301 cctatgatcg tgttttaacg gctcgtgtat ccaatgcaat ggcaaaagtc tatattg
//
LOCUS       DAOQMB010000015        14480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1517_length_14480_cov_17.901213, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000015 DAOQMB010000000
VERSION     DAOQMB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 14480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14480
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1517_length_14480_cov_17.901213"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="PLY05_01315"
     CDS             complement(<1..1241)
                     /locus_tag="PLY05_01315"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HNC01969.1"
                     /translation="MSNILLNADSYKASHYVQYPANTTVVSSYIESRGGMFEQTLFFG
                     LQAFIKQTLLKPFTQADIDEAEAVLVAHGLPFNKTGWQYILDTYNGYLPIEIQAVPEG
                     SLVNTHNVLVQVKNTDDNCAWLTSYIETALLRAVWYPTTVASVSYACKQVIARYLAQT
                     ADNMEGLLFKLHDFGSRGASSEETAALGGMAHLVNFMGSDTLSAIVAARRYYQEPMAG
                     FSIPAAEHSTITSWGRDGEQAAYANMLKQFSGQGKLVAVVSDSYDLWNAIDNLWGTEL
                     RDAVMNSGGTLVIRPDSGDPVQIVSTTIEKLMAKFGFRTNSKGYKVLPDCVRVIQGDG
                     ICLSSIEAILVEMTKRGLSADNIAFGMGGELLQKVNRDTQKFAMKASAVCVNGVWRDV
                     FKDPITDSGKRSKKGRLALVK"
     gene            complement(1481..3136)
                     /locus_tag="PLY05_01320"
     CDS             complement(1481..3136)
                     /locus_tag="PLY05_01320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC01970.1"
                     /translation="MTNQQRLDKLNALRRDYSQQLPQRIQEIKSLWSDLVSGDKAELR
                     QSFHRLIHSLAGSAGTFGYNLISEKARTIEHHLEMLFSRQPPFSSQENGQIQHSLDEL
                     QQVLEQELTKQPLVPIATNNIQLSTKKAEARLLFVLENDAEIAQEIMAQLSIYGWEVR
                     VFEHSQQISQALSAELPSAILVDIMLANNDITGTDFLLSHQQLLKNAEIPTLVLSARW
                     DWQSRLAAARAGVSAYYVKPIDFVALAERLDKLTQHHAQESFRVLIVEDETILAEHYA
                     EVLEQAGMLVKTCVSLEDMLISLEQFEPELLLLDLYMPECSGTEMAIVIRQDERFNDL
                     PIVFLSTESGRQLQLTAMQSGADDFLHKPISNADLVAAVSLRATRFRSLRQLIRQDSM
                     TGLLNHIAFKLQLEIEISRHQRTQQTMAVVMLDIDKFKQVNDVYGHPVGDRVIKSLAQ
                     LLRKRLRKTDIIGRYGGEEFAIVMTHTAEEQALQVINMIREQFGKIRHSTESAEFFCT
                     FSAGLSMFPNLQSVSTLLEASDQALYQAKAQGRNKVIAYAAYS"
     gene            complement(3129..3512)
                     /locus_tag="PLY05_01325"
     CDS             complement(3129..3512)
                     /locus_tag="PLY05_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC01971.1"
                     /translation="MSVKVLYVDDEPDIRTIVEFALEDEEGLTIQLCASGAEALAVVE
                     SYQPDIILLDVMMPSMDGPTTLQHLRTKPCCLKTPVVFVTAKVQPHEVAHFKQLGAVA
                     VIAKPFDPMTLAEQVWAIWRGCQHD"
     gene            complement(3502..6681)
                     /locus_tag="PLY05_01330"
     CDS             complement(3502..6681)
                     /locus_tag="PLY05_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNC01972.1"
                     /translation="MTIPNIQYYQHRARQYSSFAACLCMFLGCLVFVGGWAFDIELLR
                     SLLPNLVSMKANTAFAFILSGAALALHHSQLPRFWAQLISVLVLLIGGLTLLEIIFDL
                     HFGIDELFFADPYTVHGVAGRMATVSAICFILVGSALLTLKSQLTHWRWFTNSCAVLV
                     LINTFITILGYLYGINSLYQVSGYNPMAIHTALLFFIIAIGLIGFQPDQGFMRLVTAD
                     SSAGVLIRRLLPAVMFMPPLLGWLTFKGQQLSWYDISFGWALFAASNIFVFSLLVWWV
                     ARAVEQAENDKEKAEKISSWQQAILNSADLTIISTDLQGTILTCNVGALLQLGYDEND
                     IIGKNTPIIFHDPTELKQRAERLSEYLIKPIEPTFELLITQLNQGINNLEWMYIRKDG
                     SRLTVRMSITKLYSTQGELTGYLCISRDISQRKLDEQQIAAQQQELRNAYQRNQAVLD
                     FANYSIIGTDVQGVILTFNKAAEHLLGYQANEVINLHSPTLFHVPNEIAARAQVLSTE
                     LNMPVEASFETLVAKARLGTADENEWTYLCKDGKSVSVLLSVTAQHDNKGNLDGFLCI
                     ASDITERKRIERMKSEFVSTVSHELRTPLTSIRGALGLVLGKASVGMSEKAKVLLETA
                     NRNCERLTLLINDILDLEKIESGSLVFQMNTIDVVSIAQQAVVANEGYAQQHQVKLQL
                     KEGLPTAIILGDEHRLLQVFANLLSNAVKYSTINGQVEISLSQQDEYVIIAVQDHGQG
                     IPIQFRNQIFSRFAQADSSDTRAKGGTGLGLSITKAIVERHHGVISYDSTEGVGTTFY
                     VKLPLLVKPVQSVSPQAKILLCEHHPDTAQYLQQLLTQEGIATEVALDAETARLLLTQ
                     QSYRALLLDLNVLEMDEQHVKLIKELCAETESLHLPIIIISSRPQEQVKYIKEAAVNV
                     IDTLQKPVVKEQLQQAVKLALGYHYRPQILHVEDDADIIQVTQTLVEDSADYVYANNL
                     AQARMLLSQQRFDIVLLDLGLPDGSGLELLAQIPQPETRIVIFSGQEPIGVLPEAVSA
                     ILTKSKTSNESLLATLKAIMYIQETHHER"
     gene            complement(6705..8258)
                     /locus_tag="PLY05_01335"
     CDS             complement(6705..8258)
                     /locus_tag="PLY05_01335"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC01973.1"
                     /translation="MQVAQIPLNEAQRLQALQELAILDTAAEERFDRLTRLAQRYFAV
                     PTVLVSLVDANRQWFKSKQGLDVCETPREISFCGHAILQKDIFFIPDATKDPRFADNP
                     LVIGAPHIVSYAGIPLQLKNGYCVGTLCLIDYQTRQFSEEQFQTLRDLAELVQQELIL
                     KEIFDRAHAFYDNEKRLHAIVDNLLDGIVVVNQQGNIHSVNPMMEQLFACQQAELLKK
                     SLDQLILMPHLLSGLDTLSLATLPHCTNAQRQDGSIFPIEFYVKPMSQGEDVLWVCFI
                     RDISERTRIERIKSEFVATVSHELRTPLTSIRGALSLVLGKAATGLSEKAKVLLETAH
                     RNSERLGLLINDILDVEKIESGHLSLKMQVVDLVKIAQQAVIANEGYATQHQITLVLN
                     HTPDIAMVMADEHRLLQVFANLISNAIKFSYPQGTVNITLSQEPQSFRVSVRDYGQGI
                     PESFRAHIFSRFAQADSSDSRPKSGTGLGLNISQAIIEHHGGTLAYQSQEGSGAEFYF
                     VLPQLNQDI"
     gene            complement(8262..10679)
                     /locus_tag="PLY05_01340"
     CDS             complement(8262..10679)
                     /locus_tag="PLY05_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC01974.1"
                     /translation="MEQQNAKLKDRIRSVQRNFVTMLPTHINDINQHWKSLRYVAWDH
                     ERVLELQTLVHRLAGSGGTFGFPDISQASGAVDVALTQALMYDKEQIPPEAIVNLELL
                     VTNLQRVLSEVAATAENSGEELPVLTAKVEAQKALIVVIDDDLLLREHIGLVLENAGY
                     EVALFAEPPAAIHFLQEQQPALILLDLMFPNQRWPAFEVINDIRGETAQRTPVAVMSG
                     RADFRSRLQATRAGADAYLTKPLDESQLLTIAKQLTAKHLQQSWRCLVVDDDELLAQQ
                     LVEWLRQSGMIAEYVSNPRDSWLKIREFNPDVLVLDIRMPECNGIEFATMLRQDIETS
                     QLPIVFLTSENTDRTRRQAMAAGADDYLLKPIEREAFLHAVKIRARLSQRLQGQVNRI
                     IQQAPQGAGLSRHFFFNQFERTLDDANDSAVQAALVLVGLVQTVEALKQQSPVALAAL
                     QEQLLARVRALELKTWSMLGDNMIGVLLPKDTVSTHQASVKNILKQLSAQPYVIDDLS
                     VDSQMCAAVLYLGSGQTAANTLLLQAEHMLGMAIESGAGTVLEGVSGTAREAETTGKL
                     AVSRLRMLYQPIVTIADSSDPVSSVLVRLADADGNLLPAGQFLDAVEKRGWLPELDAW
                     IFNNAHHILTEQLAADTAQCLIVHASPVSLNNTLYLETVFGLLSSKPMRHPRQRLVIA
                     IPEAAAVTHRHNVEKLNQALVNAGCGLMLTGFGASTGASKILQYLQPLYVRLDQSVTK
                     RLEQTDYLDSDRQLISAIETAQAIMVADGIENAKSMSGLWAKGIRWFQGYFIHEPVAE
                     LGVNIEG"
     gene            10879..11616
                     /locus_tag="PLY05_01345"
     CDS             10879..11616
                     /locus_tag="PLY05_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNC01975.1"
                     /translation="MPNYLSEAEFLAAYNIHDYPVPLASVDVCIFTLHQQTLKVLLVK
                     RKDYPHKDRWALAGGFIDIQQDTHLENTALRTLSKKTGVTTPYLEQLATIGNGQRDPR
                     GWSMTAVYFALIPYVELLQTGTELSQWCEVNLALDLELAFDHRQLLSLALERVRAKVL
                     YTLIPAHLISSPFTLTELQRAYEIIMGHEIEKKSFRRRLEREQVLNETGQIKQEGAGR
                     PAALYTLKPECAVFNFKRQLEQLPTTN"
     gene            11706..14408
                     /gene="polA"
                     /locus_tag="PLY05_01350"
     CDS             11706..14408
                     /gene="polA"
                     /locus_tag="PLY05_01350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNC01976.1"
                     /translation="MSRKPLVLVDGSFYIYRSFFALPPLSTKDGQPTGAIKGALNALN
                     KLLKQYQPSHVAVVFDAGGPTFRHELSADYKAHRPPTPDDLKAQFAPLQDIVRAMGLP
                     VLLEQGVEGDDILGTLAVEAASHGWDVIISTGDKDMAQLVNDRITLVNPFMDTVLDAT
                     GVEAKFGVKPSQIIDYLALVGDSSDGIAGVQGVGPKTAVKWLQEYGSIANLIANAASI
                     KGKVGETFRNSLPQVAMSQQLATIDCQLALKVHPDALIRQTPNHTALKELYQRFEFNA
                     LLASVDASALPTLDEQSEPEAPPISTKYHTVTTISAWSALLDKLNLAPAFAFDTETTS
                     LNYKEARIVGFSVSLVVGEAYYVPLAHSYLGAPEQLDRDTVLKQLKPILENPHIGKIG
                     QHLKYDTHVLANHGISLQGIVFDTMLASYVLDATATRHNLDDLAKHYLNYQTTTFEEL
                     AGKGVKQLTFDQIEVEKASHYACEDADITLRLKELFVPKLLAEPSLNRLLHDIEMPVA
                     PILQQMEARGTLLDFAQLQKLSQQMGERLTELEKQAHDIAGTVFNLASPKQLGEILFD
                     KLGLEGGKKTATGQYATGENILEQLEHPLAKVILEHRSLSKLKGTYTDTLPKMANPVS
                     KRIHTSYHQAVTATGRLSSSDPNLQNIPIRSAEGRQIRQAFIAADGYQLVAADYSQIE
                     LRLMAHLSQDERLLHAFSMGEDIHKATAAEVFGIPLNEVSNEQRRNAKAINFGLLYGM
                     SAFGLAKQINASRSEAQAYIDLYFNRYTGVKRYMDNIRHQAGDKGFVETLFGRKLPMR
                     DIHSKNPSIRQAAERAAINAPLQGSAADIIKLAMIAVDAQLKLQQLDAYLLLQVHDEL
                     VLEVKNEHVERVKAMLPIAMSGVANLAVPLLVEVGVGNNWDEAH"
BASE COUNT         4266 a         3224 c         2772 g         4218 t
ORIGIN      
        1 tttttcacta aagccaaacg accttttttg ctacgtttac cactgtcagt aattgggtct
       61 ttaaacacat ctcgccaaac accattaaca cacaccgcag aggctttcat ggcaaatttt
      121 tgggtatcac gattaacctt ttgtaataac tcgccaccca tgccaaaggc aatgttatct
      181 gccgataaac cgcgttttgt catttcgact aaaatcgctt caatgctgct taaacaaata
      241 ccatcacctt gaatcacccg cacacaatca ggcaacactt tataaccttt gctgttggta
      301 cgaaaaccaa atttcgccat caacttttca atggtggtgc tcacaatttg cacaggatca
      361 ccactatcag gacgaatgac taatgtgcca ccactattca tcaccgcatc acgtagctct
      421 gtgccccaca aattatcaat cgcattccac aaatcataag aatcgctaac caccgccacc
      481 aatttgcctt gccccgaaaa ttgtttgagc atattggcat aagcagcttg ttcgccatca
      541 cgcccccaac tggtaatggt gctgtgttca gcggcaggaa tcgaaaaacc tgccatcggc
      601 tcttgataat aacgacgtgc ggcgacaatt gcgcttaacg tatctgaccc cataaagtta
      661 actaagtgcg ccatgccacc caatgcggcg gtttcttcgg agcttgcgcc tcttgagccg
      721 aagtcgtgga gcttaaaaag taaaccctcc atgttatcgg ctgtttgtgc taaataacgg
      781 gcaatcactt gtttgcaagc atacgataca ctggccactg tggtcggata ccacaccgct
      841 cttaacaaag ctgtttcgat ataactggtt aaccatgcac aattgtcatc ggtgtttttg
      901 acttgcacta agacgttgtg ggtattaacc aatgagcctt caggtacggc ttgaatctca
      961 atcggcaaat aaccgttata ggtgtctaat atgtattgcc agcctgtttt gttaaacggt
     1021 aaaccatgag caactaatac cgcttctgct tcatcaatat ctgcttgagt aaacggcttt
     1081 aataaggttt gtttaataaa agcctgcaag ccaaaaaata aagtttgctc aaacatgcca
     1141 ccacgagatt caatgtaact agaaaccaca gtggtattag caggatattg cacatagtgt
     1201 gaggctttat aagaatcggc atttaataag atgttgctca tgatggaatc cccaacattg
     1261 atttaaattt cacgactaaa tcggtctatc caatttagct tttaggatta aatagaatcc
     1321 ctacttaatc ttgatgactt aattattgtc ttaaagacac taattgtcaa gcattaaaca
     1381 aaaataattt ttagattgag acgttttgtt tttttataag gggcgttgat gttttgcatc
     1441 atcttcaccc cttgtcttct cccatgaaac tcaacccgac tcaggaataa gcggcataag
     1501 caattacctt gtttcttcct tgtgcttttg cttgatataa ggcttgatcg cttgcctcta
     1561 ataacgttga gacgctttgt aagtttggaa acattgacag ccctgcactg aatgtacaga
     1621 aaaactctgc tgattcagtg ctatggcgaa ttttgccaaa ttgttctcga atcatattaa
     1681 tcacttgtaa cgcttgctct tcggcagtat gtgtcatgac aatcgcaaac tcttcgccac
     1741 cataacgacc aataatatcg gttttacgta agcgtttacg taatagttgc gccaaacttt
     1801 tgatgacacg atcaccaact ggatgaccgt aaacatcatt cacttgttta aatttatcaa
     1861 tatctagcat aacaaccgcc atcgtttgtt gagtgcgttg atgccgagaa atttcaattt
     1921 ctagttggag cttaaaggca atatgattta ataagccagt catactatct tgacgaatca
     1981 gttgacgcag actgcgaaaa cgtgttgcac gcaaactcac ggctgcaact aaatcggcat
     2041 tactaattgg cttatgcaaa aaatcatctg caccactttg catggcggtt aattgtaatt
     2101 gacgccccga ttctgtggac aaaaacacaa tgggtaggtc attaaagcgc tcatcttgcc
     2161 gaataacgat cgccatttct gtgccactac attcaggcat atacaaatct agcaataata
     2221 attcaggctc aaattgctct aagctaatca acatatcttc aagcgaaaca caggttttta
     2281 ccaacatgcc tgcttgttct aaaacctcgg cataatgctc agctaaaatt gtctcatctt
     2341 caacaattaa gactcgaaac gactcttggg catgatgttg ggtgagttta tccaaacgct
     2401 ctgccaacgc gacaaaatca ataggcttca cataatatgc tgacacgcct gcacgtgccg
     2461 cggctaaacg actttgccaa tcccaacgtg ctgataacac aagggttgga atctctgcat
     2521 ttttgagaag ttgttgatgg cttaataaaa agtctgtgcc agtgatatcg ttgtttgcca
     2581 acataatatc aaccaaaatc gcactgggta actccgcgct caaggcctga ctgatttgtt
     2641 gactatgctc aaaaacacgc acttcccaac cataaatact tagctgagcc atgatttctt
     2701 gggcaatttc tgcatcattt tccaacacaa agagcaatct agcttcggct tttttggtcg
     2761 ataattgaat attgtttgtg gcaattggga ctaagggctg tttggtgagt tcttgctcca
     2821 acacttgctg taactcatca agtgaatgtt gtatttggcc attttcttgg ctagaaaacg
     2881 gcggctggcg gctaaacagc atttcaagat gatgctctat ggtgcgtgct ttttcactaa
     2941 tcaagttata gccaaacgtg cctgctgagc ctgccaaact atgaattaaa cgatgaaagc
     3001 tttgccttag ctctgcttta tcaccactga ctaaatcgct ccataaagac ttaatttctt
     3061 gaatacgttg gggaagttgt tggctgtagt ctctgcgtaa ggcatttaat ttatcaagac
     3121 gttgctgatt agtcatgctg acatcctcgc caaattgccc atacttgctc tgccaatgtc
     3181 attggatcaa aaggtttagc aataaccgcg actgcaccaa gttgtttaaa atgtgcaact
     3241 tcatggggtt ggactttggc agtcacaaac accacagggg tctttaaaca acatggctta
     3301 gtgcgtaaat gctgcaaagt tgtcggccca tccatactcg gcatcatcac atctaataaa
     3361 ataatatcag gctgataact ttcaacgacg gctaatgcct cagcacctga tgcacataat
     3421 tggatggtaa gcccttcttc gtcttcaagg gcaaattcca caatcgtgcg aatatctggc
     3481 tcatcatcca catacaacac tttaacgctc atgatgtgtc tcctgaatat acataatggc
     3541 tttgagggtc gctaataagg actcattact cgtcttggac ttggtgagta tggccgagac
     3601 cgcttctggt aatacaccaa ttggctcttg ccccgaaaaa atgacaatgc gcgtttctgg
     3661 ctggggaatt tgtgccaata actccaaacc cgaaccatct ggtaaaccca aatccaacaa
     3721 cacaatatca aaacgctgtt gtgacaataa cattcttgct tgcgctagat tattggcata
     3781 cacataatcc gcactatcct caaccaaggt ttgggtaacc tgaataatat cggcatcatc
     3841 ctcaacatgt aaaatctgtg gtcgatagtg ataacccaag gcaagtttga cggcttgttg
     3901 caattgctct ttgacaacag gcttttgtag ggtatcaatg acattcacag ccgcttcttt
     3961 aatatattta acctgttctt gtggccgact tgatataata ataattggca aatgcaaaga
     4021 ttctgtttcg gcacataact ctttgattag ttttacatgt tgctcatcca tttctaagac
     4081 atttaaatct aacaataatg cccgataact ctgttgcgtt aataacaaac gagctgtttc
     4141 ggcatcaagt gcgacttcgg ttgcaatacc ctcttgagtt aacaattgtt gtaaatattg
     4201 cgccgtatca ggatgatgtt cacataataa aatttttgct tgtgggctaa ctgactgaac
     4261 aggtttgact aacaaaggca atttgacata aaaggttgta cctacacctt ctgtactgtc
     4321 atacgaaatg accccatgat gacgctcaac aatcgctttg gtaatactta aacctaaacc
     4381 cgttccacct ttggcgcggg tatccgaact atctgcttgg gcaaaacgac taaaaatctg
     4441 atttctaaat tggatgggaa tgccttggcc atgatcttga acggcaataa tcacgtactc
     4501 atcttgttgg cttaacgaaa tctctacttg gccattaatg gtcgaatatt tcacagcatt
     4561 tgataacaga tttgcgaata cctgtaataa acgatgctcg tcacccaaga taattgccgt
     4621 tggtagacct tctttgagtt gcaatttgac ttgatgttgt tgagcatagc cctcattggc
     4681 gacgactgct tgttgggcaa tactgaccac atcaatagta ttcatttgaa acactaaact
     4741 gcccgactca attttttcta aatctaaaat atcattaatt aacaaggtta atcgttcaca
     4801 attacgattg gctgtttcga gtaatacttt tgctttttca gacattccca cagaggcttt
     4861 acccaaaacc aagcccaatg cacctcgaat ggaggttaat ggtgtacgca actcatggct
     4921 aactgtagag acaaactcac ttttcatacg ctcaatacgt ttgcgctcgg taatatcact
     4981 ggcaatacac aagaaaccat caaggtttcc tttattatca tgctgagctg tgacactcaa
     5041 taaaactgat accgacttgc cgtctttaca aagatacgtc cattcatttt catcggctgt
     5101 ccctaaacga gctttggcaa ctaatgtttc aaaactggct tcgactggca tatttaattc
     5161 tgttgacaac acttgtgccc gtgctgcgat ttcattaggt acgtgaaaca atgtcggtga
     5221 atgaagatta attacttcat ttgcctgata acccaataaa tgctctgctg ccttgttaaa
     5281 ggtgaggatg acgccttgaa catctgtacc aatgatactg taatttgcaa aatccaatac
     5341 tgcttggttg cgttgatacg catttcttaa ttcttgttgc tgtgctgcaa tttgttgctc
     5401 atctaatttg cgttgactaa tatctcgact aatacacaaa tagcctgtta attcaccttg
     5461 agtactgtat aacttggtga ttgacatgcg taccgttaaa cgactgccat ctttacgaat
     5521 atacatccac tccagattat taataccttg atttagttgg gtaattaaca actcaaaagt
     5581 gggttcaatt ggcttaatta aatactcaga caaacgttcg gcacgttgtt ttaactcagt
     5641 aggatcatga aaaataatgg gggtgttttt accaataata tcgttttcat catagcctaa
     5701 ttgcagcaat gctccgacat tacacgttaa aattgtgccc tgtaaatcgg tggaaataat
     5761 ggtcaaatcg gcactattta aaatagcttg ttgccatgaa ctaatttttt ctgctttttc
     5821 tttgtcattc tctgcttgct caacagcacg tgccacccac caaacaagca aactaaacac
     5881 aaagatatta ctggctgcaa ataacgccca accaaaagag atgtcatacc aagagagttg
     5941 ttgcccttta aacgttaacc accccaacaa tggtggcata aacatcactg cgggcaataa
     6001 tcgtctgatt aatacgcctg cactactgtc cgctgtgact aaacgcataa atccttgatc
     6061 aggctgaaaa ccaatcaggc caatagcaat aataaaaaac aacagtgcgg tatgaatggc
     6121 catggggtta tagccactca cttgatataa ggaattaatg ccgtataaat agcctaaaat
     6181 cgtaataaag gtattaatta acaccaatac tgcacaacta ttggtaaacc atcgccaatg
     6241 agtaagctgc gactttaacg ttaataatgc actgcctact aagataaaac aaatagccga
     6301 tacggttgcc atacgtcctg caacaccatg taccgtgtaa gggtctgcga aaaatagttc
     6361 gtctatccca aagtgcaaat caaaaataat ctctagtaat gtcaatccac ctatcagcaa
     6421 taccaataca gagatcaact gagcccaaaa acgtggaagt tgactatggt gtaaggccag
     6481 tgcggctcca gacaagataa aggcaaatgc cgtattagct ttcattgaca ctaaattggg
     6541 caacaaactc cgtaatagct ctatatcaaa tgcccatcca ccaacaaata ctaaacagcc
     6601 caaaaacata cataagcaag cggcaaatga tgaatattga cgtgcacgat gctgataata
     6661 ttgtatgttg gggattgtca ttttttattc ctaaaagtgg ggatttaaat gtcttgattt
     6721 aactgaggta agacaaaata aaactctgca cctgatcctt cttgtgattg ataggccaat
     6781 gttccaccat gatgttcaat gattgcttgg ctaatattta aacctaaacc cgtgccactt
     6841 ttaggacggc tatccgaact atctgcttga gcaaaacgac taaaaatatg tgctctaaac
     6901 gattctggaa tgccttgacc ataatctctg acactgaccc taaaactttg tggttcttga
     6961 ctgagcgtga tattcaccgt gccttgagga taagaaaact taatagcatt agaaatcaaa
     7021 ttggcaaaca cttgtaataa acgatgctca tcagccatca ccatagcgat gtctggcgta
     7081 tggttaagca ccaacgtaat ttgatgttgt gtggcataac cttcattggc aatcactgct
     7141 tgttgtgcaa tttttaccaa atcaacaact tgcattttta agcttaaatg gccagattca
     7201 attttttcca catctaaaat atcattaatc aataaaccta aacgctcact gttgcgatgt
     7261 gcagtttcta ataatacttt ggctttttcg gataaaccgg ttgctgcttt acctagtacc
     7321 aatgacaatg caccacgaat cgaggttaag ggcgtgcgta actcatggct gacagtcgca
     7381 acaaattcac ttttaattcg ctcaatacgt gtacgctcac taatgtcacg aataaaacac
     7441 acccacaata catcttcacc ttgcgacata ggcttaacat aaaattctat aggaaaaatt
     7501 gaaccatctt gacgctgggc atttgtgcaa tgtggtaatg tggccagtga cagtgtgtct
     7561 aaaccactta ataaatgtgg cattaaaatc agctgatcca aagatttctt gagcaactct
     7621 gcttgctgac aagcaaataa ttgctccatc atcgggttaa cactgtgaat gttgccctgt
     7681 tgatttacca ccacaatccc atctaaaaga ttatccacaa tggcatgtaa gcgtttttca
     7741 ttatcataaa atgcatgagc acgatcaaaa atttctttta aaatcaactc ttgttgtaca
     7801 agctcggcca aatcgcgcaa ggtttggaat tgttcctcac taaattgccg tgtttgataa
     7861 tcaatcaggc ataacgtgcc aacacaataa ccatttttta actgaagagg gataccagca
     7921 taagagacaa tatgaggtgc accaatgact aagggattgt cagcaaaacg gggatctttt
     7981 gtggcatcag gaataaaaaa aatatctttt tgcaaaatcg catgaccaca aaacgatatc
     8041 tctcgcggcg tttcgcaaac atccaaaccc tgtttggatt taaaccattg ccgattggca
     8101 tctacaaggc tcactaatac ggttggtacg gcaaaatagc gttgtgccaa acgagtcaaa
     8161 cgatcaaaac gctcttctgc ggctgtatct aaaatggcta gctcttgtaa agcttggagc
     8221 cgttgcgctt catttaatgg tatttgagca acttgcatca gttatccctc aatattaacc
     8281 cctaactcag caacgggttc atggataaag tagccttgaa accaacgaat cccttttgcc
     8341 cataagcccg acatactctt ggcattttca ataccatccg ccaccataat cgcttgtgca
     8401 gtttcaatgg cactgattaa ttggcgatct gagtctaaat agtctgtttg ttctaagcgt
     8461 ttggtgacac tttggtctaa gcgtacatac aagggttgta ggtattgcaa aatcttactc
     8521 gcccctgtcg atgcgccaaa tccagttaac atcaaaccac aacctgcatt caccaatgct
     8581 tgattgagct tttcaacatt atgacgatgc gtcactgcgg cggcttcagg aatcgcaatc
     8641 accaaacgtt gacgcggatg acgcatcggt ttagaggata acaaaccgaa gactgtttcg
     8701 agatacaacg tgttgtttaa actcacggga ctggcatgaa caatcaaaca ttgtgccgtg
     8761 tcagccgcta attgttcggt caatatatga tgggcattgt taaagatcca tgcatctaac
     8821 tcaggcaacc atccccgctt ttcaacggca tccaaaaact gacccgcagg taacaagttg
     8881 ccatccgcat ccgccaaacg caccaagaca ctactcacag ggtcactact gtcggcgatg
     8941 gtcacaatcg gctgatacaa catgcgtaag cgactaactg ccagtttgcc tgttgtttcg
     9001 gcttctcttg ctgtaccact aactccttct aaaaccgtgc cagcacctga ttcaattgcc
     9061 atgcccaaca tgtgttctgc ttgtaataac aaagtgtttg cggctgtttg gccactgcct
     9121 aaatataaaa ctgcggcaca catttgactg tctacgctta aatcatctat gacatatggc
     9181 tgggcagata actgttttaa gatatttttg acgctggctt gatgggtact taccgtatct
     9241 ttgggcaaca gcacgccaat catgttatca cccaacattg accacgtttt taactctaaa
     9301 gctcggacac gcgccaataa ttgctcttgt aaagccgcca aggcaacggg gctttgttgc
     9361 tttaatgcct ccactgtttg caccaatccc actaacacta aggctgcttg tactgcactg
     9421 tcattggcat catccaatgt ccgttcaaat tgattaaaga aaaagtggcg agataaccct
     9481 gcaccttgag gagcttgttg aataatccga tttacttgac cttgtaaacg ttggcttaaa
     9541 cgagcgcgaa tttttacggc gtgcaaaaat gcttctcgct caatcggttt gagcaaataa
     9601 tcatctgcac ctgcggccat ggcttgtcgg cgagtgcgat cggtgttttc cgaggttaaa
     9661 aacacaattg gcagttgtga tgtttcgatg tcctgacgca acatggtcgc aaattcaatg
     9721 ccattacatt cgggcattcg aatatccaat accaacacat cagggttaaa ttctcgtatt
     9781 tttagccaac tgtcacgtgg gttactgaca tattctgcaa tcatgccact ttgtcttagc
     9841 cactccacta attgttgtgc caataattca tcatcatcta ccactaaaca acgccagctc
     9901 tgttgcaaat gctttgctgt caactgtttg gcaattgtta ataattggct ttcatctaag
     9961 ggcttggtta aatatgcatc cgctccagca cgtgttgctt gtaatcttga gcgaaaatca
    10021 gcacgtccac tcatcactgc tacaggcgta cgttgggctg tttcgccacg aatatcgtta
    10081 attacctcaa aggctggcca acgttgatta ggaaacatta aatctaacag aatgagtgct
    10141 ggttgttgct cttgtaaaaa gtggattgcc gcaggtggct cagcaaataa ggcaacctca
    10201 taacctgcat tttctagtac taaaccaatg tgctcacgca ataataagtc atcatcaatc
    10261 accacaatta atgctttttg cgcttctacc ttggccgtta gcacaggtaa ttcttcgccc
    10321 gaattttcag cagttgccgc gacttcgctt aacacacgtt gtaaattcgt tactaaaagc
    10381 tcaagattaa ctatcgcttc tggtggaatt tgctctttgt catacatcaa agcttgggtt
    10441 aatgcgacat ctaccgcccc cgaagcctga ctaatatcag gaaaaccaaa cgttccgccc
    10501 gaaccagcca aacgatgcac caaggtttgc agttccaaca cacgttcatg atcccacgcc
    10561 acataacgca aactcttcca atgttgatta atatcattaa tatgtgtggg tagcatggtg
    10621 acaaaattac gttgtacgct acgaatacga tcttttaatt tggcgttctg ctgttccata
    10681 atacactcta ccaacattca ttaacgtgat aacgacttat atagttaccg cttatttgct
    10741 tgtatatcaa tggttttatg ctcatcaaac ataatacccg agtcttatac taggattatt
    10801 gattgattat gcactagaat caaaaaaata cgtatgaaca atgttttaaa ttatttctga
    10861 ttaataaggg ctaaactctt gccaaactat ctttctgaag ccgaattttt ggcggcttat
    10921 aatattcatg attatcctgt gccattggct tcggtggatg tctgtatttt taccttgcac
    10981 caacaaacct taaaagtgtt attggttaaa cgtaaagatt atccccacaa agatagatgg
    11041 gcgttggcag gtggttttat tgatattcaa caagatacgc acctagaaaa taccgcctta
    11101 cgaacattaa gcaaaaaaac tggcgtgacc acaccctatt tagaacaatt agcgactatt
    11161 ggtaatgggc aacgtgatcc tcgcggttgg tcaatgacgg cggtgtattt tgccctcatt
    11221 ccttatgttg agttattaca aacaggcaca gagctcagcc aatggtgtga ggtgaatttg
    11281 gctttagact tagagttggc ttttgatcat cgtcaattat taagcttagc tttggagcgt
    11341 gttcgcgcta aagtgttata taccttaatt cctgcccatt taattagctc gccttttacc
    11401 ctcaccgaac tacaacgcgc ctatgaaatt attatgggac atgagattga gaaaaaatcg
    11461 tttagacggc gtttggaacg cgaacaagtg ttaaatgaaa ctggtcaaat taaacaagaa
    11521 ggggctggcc gacctgctgc cttatatacc ttaaagcctg aatgtgcggt atttaatttt
    11581 aaacgacagt tggaacaact accaacaacg aattaatttg tcgtcttaaa tatagaaatc
    11641 gtttatctaa accctaagcc gtctataatt cggccttatt acatttgttt attggatacg
    11701 ccctcatgtc tcgtaagccg cttgttttgg tcgatggttc attttatatc tatcgctctt
    11761 tttttgcttt acctcccctc tctaccaaag acgggcaacc aacaggggct atcaaaggcg
    11821 cactcaatgc cctcaataaa ttactcaaac aatatcaacc tagccatgtt gctgtcgttt
    11881 ttgatgcagg tggcccgact tttaggcacg aattatctgc cgattacaaa gcacaccgtc
    11941 caccgactcc tgacgactta aaagcacaat ttgccccttt gcaagacatt gtgcgtgcaa
    12001 tgggtttgcc tgtgttatta gaacaaggcg ttgaaggcga tgatatttta ggtacattag
    12061 ccgttgaagc agccagtcat ggttgggatg tgattatctc aacgggcgat aaagacatgg
    12121 cacaattggt gaatgaccgt attacccttg ttaatccgtt tatggatacg gtgctagacg
    12181 caacaggcgt agaggcaaag tttggcgtta aaccgagtca aattattgat tatttggctt
    12241 tagttggcga ctcttctgat ggtattgcgg gtgtgcaagg tgtagggcca aaaacggcgg
    12301 tgaaatggtt gcaagaatac ggctcaattg ccaatttaat tgccaatgcc gccagtatta
    12361 aaggtaaagt cggtgaaacc tttagaaata gcttgccaca agtcgcgatg agccaacagc
    12421 tcgccaccat tgattgtcaa ttagccttaa aagtgcatcc tgatgcgctc attcgtcaaa
    12481 cacccaatca cactgcttta aaagagcttt atcaacgttt tgaatttaat gctttattag
    12541 cgagtgtaga tgcgtctgct ctaccgacac tggatgaaca aagtgaaccc gaagccccac
    12601 cgattagcac taaatatcat acggttacga caatttcggc atggtctgcc ctgttagata
    12661 aacttaactt agcccctgcg tttgcctttg ataccgaaac cactagccta aattataaag
    12721 aagcgcgtat tgttgggttt tcggtgtctt tagtggtggg tgaagcttat tatgtaccgc
    12781 ttgcacatag ttatttaggt gcgcccgaac aacttgacag agatacggtg ttaaaacaac
    12841 tcaaacccat tttagaaaat ccacatattg gcaaaattgg ccaacaccta aaatatgata
    12901 ctcatgtgtt agctaatcat ggcatttcct tacaagggat tgtttttgat accatgctcg
    12961 cttcttatgt gttagatgcc accgctactc gccataattt ggatgattta gccaaacatt
    13021 atcttaacta tcaaaccacg acttttgagg aattagcagg caaaggcgtc aaacaactga
    13081 cgtttgacca aattgaagta gaaaaagcca gccattatgc ctgtgaagat gcggatatta
    13141 ctttaagact caaagagtta tttgtgccta aattattggc tgagccatca ctcaatcgtt
    13201 tattgcacga tatcgaaatg cctgttgcgc cgattttgca acagatggaa gcgcgaggca
    13261 ctttattaga ctttgcccaa ttacaaaaac ttagtcagca aatgggtgaa cgtttaactg
    13321 aacttgaaaa acaagcacac gacattgcag gcacagtgtt taacttagcc tcacctaaac
    13381 aattaggtga aattttattt gataaattag ggttagaagg gggcaaaaaa accgcgacag
    13441 gccaatatgc aacaggcgaa aatatcttag agcagttaga acatccttta gctaaagtga
    13501 ttttagagca tcgcagttta agtaaattaa aaggcactta taccgatact ctgcccaaaa
    13561 tggctaatcc cgttagcaaa cgtattcata cctcgtatca tcaggcagtc actgccacag
    13621 gtcgtttgtc atcgtcagac cctaatttac aaaacattcc tattcgtagt gctgagggtc
    13681 gccaaattcg ccaagccttt attgccgcag acggctatca attagtggct gccgactact
    13741 cgcaaattga attacgctta atggcacatt taagccaaga tgaacgctta ttacacgcct
    13801 ttagcatggg cgaagacatt cacaaagcca ccgctgccga agtctttggt attcctctca
    13861 atgaggtcag caacgaacaa cgccgtaacg ctaaagcgat taactttggt ttgttatatg
    13921 gtatgtcggc ctttggttta gctaaacaaa tcaatgcctc acgtagcgaa gcccaagcct
    13981 atattgattt atattttaat cgttatacag gcgttaaacg ttatatggac aatattcgtc
    14041 atcaagcggg tgacaaaggt tttgtcgaaa ccttgtttgg tcgcaaatta ccgatgcgtg
    14101 atattcattc taaaaacccg agcattcgcc aagcggcaga acgtgctgcc attaatgcac
    14161 ccttacaagg ttcggcagca gacattatta aattagccat gattgccgtt gatgctcaac
    14221 tcaaactgca acaattagac gcttatttgt tattacaagt tcatgacgaa ttggtgctag
    14281 aagtcaaaaa tgagcatgta gaacgcgtca aagccatgtt gccgattgcc atgagtggtg
    14341 tagccaattt agctgtaccg ttattggtgg aagtgggtgt tggcaacaat tgggatgagg
    14401 cacattaatt tttatcaaag acacaagatg tcgttcacat ccccataata cttgtttgtt
    14461 acaatgttta tgctatttta
//
LOCUS       DAOQMB010000016        14327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1547_length_14327_cov_14.696258, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000016 DAOQMB010000000
VERSION     DAOQMB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 14327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14327
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1547_length_14327_cov_14.696258"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..950
                     /locus_tag="PLY05_01355"
     CDS             27..950
                     /locus_tag="PLY05_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004815237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC01977.1"
                     /translation="MTTALVIENLTKTYKNGFQALKGINLRVEQGEFYALLGPNGAGK
                     STTIGIISSLVKKTTGNVSVFGYDLDQDLSLAKQQLGVVPQEFNFGQFEKVVDIVATQ
                     AGYYGIDKKLAYERAEKYLKKLGIWDKKDQQARMLSGGMKRRLMIARAMVHEPKLLIL
                     DEPTAGVDIELRRSMWEFLTEINQQGTTIILTTHYLEEAEMLCRRIAIIDKGQIVEDT
                     DMKTLLSKLDVEHFLLDVREPLAQAPHIDNIPTKLIDERTLEVAVNKSQSLNEVFNVL
                     NAKQINVLSLRNKSNRLEELFVSMVEKNLNK"
     gene            963..1736
                     /locus_tag="PLY05_01360"
     CDS             963..1736
                     /locus_tag="PLY05_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC01978.1"
                     /translation="MTNQQIYIALQTILTKEIRRFTRIWVQTLLPPAITMSLYFVIFG
                     NIVGSRIGQMGGFDYMQYIVPGLIMMSVITNSYSNVVSSFYSAKFQRSIEELLVSPVP
                     HHIILTGYVLGGVARGLLVGLIVTILSLFFTHLSLHHLLVIIYSVFLTATLFSIGGFI
                     NAVFAKSFDDISIVPTFILTPLTYLGGVFYSIENLSPFFQNVSLFNPIVYMVNTFRYG
                     ILGVSDVNVGLSLTMMTALTIGLYFYALRLLSSGRGMKE"
     assembly_gap    1917..2016
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2017..2433
                     /gene="queF"
                     /locus_tag="PLY05_01365"
     CDS             <2017..2433
                     /gene="queF"
                     /locus_tag="PLY05_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009586505.1"
                     /note="catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNC01979.1"
                     /translation="QLQGQCLDNLEIECDDYVLNPTILSCQPAAQIVSESLYSHLLRS
                     NCPVTNQPDWGSVQISYTGYALNHENLLRYLISFRQHNDFHEQCVERIFTDIMQYCQP
                     QQLTVQAHYTRRGGLDINPYRSNCQPFVEFQRLARQ"
     gene            2571..3245
                     /locus_tag="PLY05_01370"
     CDS             2571..3245
                     /locus_tag="PLY05_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01980.1"
                     /translation="MATYFGFEPSTGLKADIDKLLANLANKAPEAQYHLANEISLKLT
                     DEIIDVLLLNLVDLMQHHGEGEGGGLLKFLGGFLKKTMHGMLKLMLGKADNAEVNKRA
                     DYLRARSLALPNDVARIGFKLDADTYQHFMHAFAQIEAGNGKAVTQELVKTMQVFNEA
                     CIVSFFDEFVAVLNLGMINRKGASVTRGLIQKESNSTVEKLIPSLTDQQLKDLAAYFK
                     AMLVER"
     gene            3252..3917
                     /locus_tag="PLY05_01375"
     CDS             3252..3917
                     /locus_tag="PLY05_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01981.1"
                     /translation="MTTYYAIPCPQGLRENFAKLLVNFEQNSKEPQNQLLIQIANDYT
                     DKIIEVMVLGNSHMMDQEAFAVKVLHNVASAIKTTAHTLIKQVLSKMKNEEMSPLASQ
                     IRARKLVINNQEYISFPVPEHLAQSYKYCFAQIKLGDVSVKHKLMESMLEFSSLANHY
                     FYVESIKPLKLGFVTRKIADLGGVTIDKASHAAIKKLVPQLNKEELQGFVEYLEPLFI
                     DVN"
     gene            4061..4648
                     /locus_tag="PLY05_01380"
     CDS             4061..4648
                     /locus_tag="PLY05_01380"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNC01982.1"
                     /translation="MTQIIFAIIGKYPPLSVQQIIQQQPQAIVILGGGLYRGGEYNNQ
                     YQSGGASTARLQYGAYLAKQTRLPIGLSGMEVAYGMSNTLGQFGLAATWLENNSYDTH
                     ENAQFSAQLLIKNNIQKIVLVTDAWHMSRSVLAFRQAGFNVIPAPTEFPDGFYINKKQ
                     WWQANLNTYMQNLRGWTEIFGHVKYRVRYALFSKA"
     gene            complement(4624..5517)
                     /locus_tag="PLY05_01385"
     CDS             complement(4624..5517)
                     /locus_tag="PLY05_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019518996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNC01983.1"
                     /translation="MRILLTGGTGFLGQALCAELLKQQHQLTIFTRQPDKVTKQYQGK
                     VQAVNQIEHLNPNSQFDVVINLAGEGIADKPWTNKRKQALYDSRVSLTKDLVNWLKQC
                     SHQPQLLISGSAIGWYGNQNNNRVDENSAPHQEFVHDLCHAWEQAALTMQSPSTRVCI
                     IRTGVVLAAQGGMLKRLLPVFKLNLGGRLGNGQQWFSWIGLDDFIAAILFLIHHPQVD
                     GIFNLTAPHPVTNQQFTQIFAQHLHRIAPFPVPSIVLKLLLGEMSTLLLDGQPVIPQR
                     LINLDFDFKSPTLDQALLNNA"
     gene            5773..6960
                     /gene="zigA"
                     /locus_tag="PLY05_01390"
     CDS             5773..6960
                     /gene="zigA"
                     /locus_tag="PLY05_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009586727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HNC01984.1"
                     /translation="MLPVTVLSGFLGAGKTTVLQHILHNRQGLKVALIVNDMSEINID
                     AALVAGEASLNYQQEKLVEMSNGCICCTLREDLLIEVSELAKSGKFDYLVIESTGISE
                     PLPIAETFTFRDEQGVSLSDIARLDTMVTVVDAVNFTKDYFAAQSLAEMDENLGEDDE
                     RTLSDLLVEQVEFADVILVSKIDLVSSQEVENLLAVLRTLNTQAKIYPIQQGQVDLEK
                     IINTQLFSFEQAQQAAGWLKEMRGEHIPESEEYGIASVNFVARRPFHPQRFWNFLYQD
                     WQQEQGRLLRAKGYFWLSSRLKMAGVMSQAGGVLRHGGAGYWWAAVPETEWPTEQDYL
                     ARINEKWDEEVGDCRQELVFIGQNLPSDILLKQLHTCLLTEEEMAEGIEYCEKLPDPF
                     PEW"
     gene            7011..>7913
                     /locus_tag="PLY05_01395"
     CDS             7011..>7913
                     /locus_tag="PLY05_01395"
                     /inference="COORDINATES: protein motif:HMM:NF023426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC01985.1"
                     /translation="MRILFIHQNFPAQFKHLAPALAADKNNQVLAMHMRSDMPKNWQG
                     VQMLSYAAKRGSTPKIHPWITDIETKVIRGEAAFYAAMQLKKAGFTPDVIIAHCGWGE
                     SLFVKDVWPTAKLAIYSEFYYHAQGADVGFDPEFPSSLTDQCRIRVKNTNNLLHFEVA
                     DAGISPTYWQASTFPQPFRDKITVVHDGIDTQAIKPNPTIQMNFKNAQGEITLSRNDE
                     VITFVARNLEPYRGYHIFMRALPDILRQRPTAKVLIIGADGVSYGKEAPQGQTWKNIF
                     INEVKEQVDWSRVFFLGQVPYPQFI"
     assembly_gap    7914..8013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8118..9125
                     /locus_tag="PLY05_01400"
     CDS             8118..9125
                     /locus_tag="PLY05_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HNC01986.1"
                     /translation="MQPILSAIPTNIITGFLGSGKTTLIQQLLASKPESERWAVLVNE
                     FGQIGIDQALIGQQQEVFIKEVAGGCICCANFLPMQVALSQLLAKAKPQRLLIEPTGL
                     GHPKQIIDSLSASHWQSTLKLEAVLCLIDARQYQDQRVREHESFIAQVEVADVLVFSK
                     ADLLNVEQKQLMLDFAQQLPSPNAKTVFVEHGQLDHELLGYKRQQIPVLKRSLLHDNL
                     RLNAVQNTASTDIVEPPYHYHQQALDTYVGGWIFPVDWQFEHHALMDLLFAIKAQRVK
                     GVIHTNQGWLAVNLTAVDASFNSCAESFDSRLEIISANNIDWSALEQALLAIRSNPTH
                     C"
     gene            complement(9150..10181)
                     /locus_tag="PLY05_01405"
     CDS             complement(9150..10181)
                     /locus_tag="PLY05_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HNC01987.1"
                     /translation="MKNIYRDVEHPFAQYIRIIGKGKTGSRSLSFEEAQAAFAMIVRG
                     EVLEAQLGAFLMLLRVKEESPEELAGFVAATREWLQAPHIAVDLDWSSYAGKRKHYPW
                     FLLAALVLAENGVRIFMHGASGHTINRLYTEEVLPVLGYGLCQTWADVELQLNQKNFA
                     YMSLDAYCPPLGNIIQLRNIMGLRSPVHTLARLINPLNAKCSLQAIFHPAYRESHQQA
                     AKLLNYSNSMVIKGEGGEFERNPDGRCLVKGIKNGELYDLEWPMMFEERHNAEDEFSL
                     DDFIAVWRGQKAHEYGESAVIGTLALALIGLQRCNDPEEALTLAQQWWHNRKAKQKIK
                     AGEQMDLFA"
     gene            complement(10200..10535)
                     /locus_tag="PLY05_01410"
     CDS             complement(10200..10535)
                     /locus_tag="PLY05_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HNC01988.1"
                     /translation="MSAVLAGTDIGVDEEGYLLNHHDWTPDIATQLAQQQQLILHDEH
                     WQILYAIRDFYQQYQRSPTTRVLLKYLGQQLGSEKSSSIYVMSLFGDGTPAKTIARLA
                     GLPKPTNCI"
     gene            complement(10510..10794)
                     /locus_tag="PLY05_01415"
     CDS             complement(10510..10794)
                     /locus_tag="PLY05_01415"
                     /inference="COORDINATES: protein motif:HMM:NF016003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsrH/TusB family sulfur metabolism protein"
                     /protein_id="HNC01989.1"
                     /translation="MPTLHLISTTLSNELYQQLITILSDDDVLLFTNQGVYNLYSQQD
                     LLKKYRCYALMSDLQAYGLLTLVEQTSCKIIDYPEFVQLGILYERSISWH"
     gene            complement(10798..11151)
                     /gene="tusC"
                     /locus_tag="PLY05_01420"
     CDS             complement(10798..11151)
                     /gene="tusC"
                     /locus_tag="PLY05_01420"
                     /inference="COORDINATES: protein motif:HMM:NF001238.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HNC01990.1"
                     /translation="MKPLFIHHTAPYHGHHAQESLDALLVMAAFGQNPSVLFQGDGVW
                     QLLANQQSEIVGKASIIAQLSALELYDVSEVYVDEQSLLTRNLKAEHLAIDVQILSQE
                     SLKDFFQQHSPIIRL"
     gene            complement(11153..>11549)
                     /gene="tusD"
                     /locus_tag="PLY05_01425"
     CDS             complement(11153..>11549)
                     /gene="tusD"
                     /locus_tag="PLY05_01425"
                     /inference="COORDINATES: protein motif:HMM:NF001237.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HNC01991.1"
                     /translation="IEYYFMKFVLLITAPIESSNAWHAYYFAKTALEQGHQVSPFFYN
                     DGVTVATRLILNHDNANSLNQCWQNLAKQHQLELAVCVAAALRRGITDADNSHRHHLQ
                     GDNLADGFTLTGLGTLTDLMINSDRFLQF"
     assembly_gap    11550..11649
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11679..13169
                     /gene="glpK"
                     /locus_tag="PLY05_01430"
     CDS             11679..13169
                     /gene="glpK"
                     /locus_tag="PLY05_01430"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HNC01992.1"
                     /translation="MQPYLLSLDQGTTSTRAIIFNLQGQAVARAQLELKQYFPADGWV
                     EHDAEEIAQSSLAVCREALQQSGITAQQIAAIGITNQRETTVVWDRKTGKPIHKAIVW
                     QDRRTADTCLKLKNRGHETMVQQKTGLLLDPYFSASKLAWILDNVSGARKRAEKGELA
                     FGTIDTWLIWQLTGGKKHATDATNAARTLLFNIHTQEWDEELLKLFNIPVSLLPVVQD
                     SASDFGLTETRLFGAPMQIGGVAGDQQAALVGQACFNSGMVKSTYGTGCFMVMNTGDE
                     AINSTHRLLTTVAYRLNGKVTYALEGSIFVAGAAVQWLRDGIQLIKQAGDTEAIAEET
                     MDSRGVYLVPAFTGLGAPYWDPHARGAIFGLTRDTGIGEIVIAGLEAVCFQTRDLMEA
                     MKKDGATKLKTLRVDGGMAVNNWLMQALSDIVGVAVDRPTVTETTALGVAWLAGLQVG
                     KFASLDAIAELWHCQREFTPKMDIDKRETMYAGWLDAVQRVTLKTV"
     gene            13348..>14327
                     /gene="galE"
                     /locus_tag="PLY05_01435"
     CDS             13348..>14327
                     /gene="galE"
                     /locus_tag="PLY05_01435"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003664995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HNC01993.1"
                     /translation="MILLTGGAGYIGSHTALELLAAGFDILVLDNLSNSKLEAINRTR
                     QLANKNFEFIEGDIRDRQLLDKIFKSYPITAVVHFAGLKAVGESVQKPLYYYDNNVSG
                     SVVLFEAMTAANIKTIVFSSSATVYGDPHTVPIKEDFPLSTTNPYGASKLMIENILRD
                     VKVADNSWRIALLRYFNPTGAHESGLIGEDPNGIPNNLMPYIAQVAVGKRSHLQVFGG
                     DYPTHDGTGVRDYIHVVDLAKGHVKALQYLEQNQELVTVNLGTGKGYSVLDMLKAMEK
                     AAGKTIAYQIVERRAGDIASCYADPQYAFNKIGWQAEFDQERMCSDMWRW"
BASE COUNT         4286 a         2485 c         2928 g         4328 t
ORIGIN      
        1 gtgggagagg gtcggggtga ggggttatga caacagcatt agtgatcgag aatctaacca
       61 aaacctacaa aaatggtttt caggccttaa aaggcattaa tttacgtgtt gaacaaggcg
      121 agttttacgc gctcttaggg ccaaatggcg caggtaaatc aacaacgatt ggcattatta
      181 gttctttagt caaaaagacc acaggtaatg tctcggtatt tggttatgac ttagaccaag
      241 atttatcttt agcgaaacaa caactgggtg ttgtgcctca agagtttaac tttggtcagt
      301 ttgagaaagt ggttgatatt gtcgctacac aagcaggtta ttatggcatt gataaaaaat
      361 tagcctatga gcgtgccgaa aagtatctca aaaaactggg tatttgggat aaaaaagacc
      421 aacaagcacg gatgttatca ggggggatga agcgacggtt aatgattgcc cgtgcgatgg
      481 tgcatgagcc aaaactattg attttggatg agccaaccgc tggcgtggac attgagttaa
      541 gacggtcgat gtgggagttt ttaaccgaaa ttaatcaaca aggtacaacg attattttaa
      601 ccacccatta tttagaagaa gcagaaatgt tgtgtcgccg tattgccatt attgataaag
      661 ggcaaattgt tgaagacacc gacatgaaaa ctttgctcag taaactagat gttgaacact
      721 tcttattgga tgtgcgtgaa cccttagccc aagccccaca cattgacaat attccgacta
      781 aattaattga cgaacgaacc ttagaggtgg cggtaaacaa aagccaatcg ctgaatgagg
      841 tatttaatgt actcaatgcc aaacaaatta atgtattgag tttacgtaat aaatccaatc
      901 gtttagaaga attatttgtc agtatggttg agaagaatct caacaaataa tccgagttta
      961 ttatgactaa tcagcaaatt tatattgcct tacagactat tttaaccaaa gaaatccgcc
     1021 gatttacccg aatttgggtg caaacattgt taccacctgc cattaccatg tctttatatt
     1081 ttgtcatttt tggcaatatt gttggcagtc gtattggtca aatgggtggt tttgactata
     1141 tgcaatacat tgttcccggt ttgattatga tgtcggtgat taccaactct tattcaaatg
     1201 tggtgtccag tttttatagt gctaagtttc aacgcagtat tgaggaactc ttagtctcgc
     1261 ccgtgccaca tcatattatt ttaacgggtt atgtgttggg tggcgtggcg cgtgggttgt
     1321 tggtggggtt aattgtgacc atactttctt tattttttac ccatttatca ctgcatcacc
     1381 tattagtgat tatttactct gtgtttctga ctgccacatt gttttcaatt ggtggtttta
     1441 ttaatgcggt atttgctaaa tcttttgatg atatttcgat tgtgccaacc tttattttaa
     1501 cgccattaac ctatttgggt ggggtgtttt attccatcga aaatttatca ccattctttc
     1561 aaaacgtctc gttatttaac ccaattgttt atatggtgaa tacctttaga tacggcattt
     1621 taggggtaag tgatgttaat gttggccttt ctttaacgat gatgacggcc ttaaccattg
     1681 gcttatattt ttatgcttta cgtttgttgt cttcaggacg aggcatgaag gagtaatcgc
     1741 atgagtttta attatcagca aactggttta ggtcaagaaa ccgattacgc ctcagattac
     1801 aacccacaat tattgtgtcc attaccacgc agccaaggac gtcaagaatt agcattaagt
     1861 aaagacttgc cgtttcaggg ggttgatgtt tggcataatt atgaagcttc gtggctnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncaat tacaagggca atgtttggat
     2041 aatttagaga ttgaatgtga tgattatgtg ttaaatccga ccatactcag ctgtcagccc
     2101 gccgcacaaa ttgtctccga atcgctctat tcgcacttgt tacgcagcaa ttgccctgtc
     2161 actaatcagc ctgattgggg cagtgtgcaa attagttata caggttatgc gcttaaccac
     2221 gaaaacttat tacgttactt gatctctttt aggcaacata atgattttca tgaacaatgt
     2281 gttgagcgaa tatttaccga tattatgcaa tattgccaac cacaacaatt gacagtgcaa
     2341 gcacactaca cacgacgcgg aggcttggat attaatccat accgtagcaa ttgccagcca
     2401 tttgttgagt ttcagcgttt ggcgcggcaa taattgttag gcaattgttt aaaaactcgg
     2461 atttttgtca tttgttcgca attttacctt gccaatcaac ggtatacttg ccaaaatcca
     2521 atccttaatt aaggaaaata tcctaaaatc aacccaaaga ggaaacaata atggcaactt
     2581 attttggttt cgagcctagt acaggtttaa aagcagatat tgataaattg ttggctaatt
     2641 tagctaataa agcaccagag gcacagtatc acttggcaaa tgaaatttct ttaaaactta
     2701 ctgacgaaat tattgatgtt ttgttattaa atttagtcga tttaatgcaa catcatggtg
     2761 aaggtgaagg cggtggtttg ttaaagtttt taggtggttt tttaaagaaa actatgcatg
     2821 gtatgttaaa actcatgtta ggtaaggctg ataatgccga agttaacaaa cgtgcagatt
     2881 atttacgtgc gcgttctttg gcattaccta atgatgttgc gcgtattggt tttaaattag
     2941 atgccgatac ctatcagcat tttatgcacg cctttgcaca aattgaagca ggtaatggca
     3001 aagcagtcac tcaagaattg gttaaaacca tgcaagtatt caacgaagct tgtatcgtat
     3061 ctttttttga tgaatttgtt gcggtattaa acttaggtat gattaatcgt aaaggcgcaa
     3121 gtgtgacacg cggattaatt caaaaagaaa gtaattctac ggttgaaaaa ttgatcccat
     3181 ctttaacaga ccagcaatta aaagatcttg ctgcctactt taaagcaatg ttagtagagc
     3241 gttaatcagc aatgacaact tattatgcaa taccttgccc acaaggatta cgtgaaaatt
     3301 ttgccaaatt actggttaat tttgagcaaa acagcaaaga gccacaaaat cagttattga
     3361 ttcaaattgc caatgactat actgataaaa ttattgaagt aatggtttta ggaaatagtc
     3421 atatgatgga tcaagaggct tttgcggtga aggtgttgca taatgttgct agtgcgatta
     3481 aaacaacggc gcatacctta attaaacaag tcttatcaaa gatgaaaaat gaggaaatgt
     3541 cgccgttggc ttcacaaatt cgtgcgcgta aattagttat taacaatcaa gaatatataa
     3601 gttttcctgt gccagagcat ttagcacaaa gttataaata ttgttttgca caaataaaac
     3661 ttggtgatgt ttctgttaaa cataaactca tggagtcgat gttagagttt tcttcattgg
     3721 ctaaccatta tttttatgtg gagtcaatca aacctttaaa attaggtttt gttacccgaa
     3781 aaattgctga tttaggaggc gtcactattg ataaagcctc tcatgcagca attaaaaaac
     3841 tagtgccgca attaaataaa gaagaattac aaggatttgt ggaatattta gaacctttgt
     3901 ttattgatgt taattaatga agtttttatt aagttttcct gtttttatca gttttctatt
     3961 attgtttgct ttggtagcac tacattacca acgtaaacgc tgggcttatt gtttaatcat
     4021 atcagcaatc ataagcaatt atttatttgc aacgccttta atgacccaga ttatttttgc
     4081 gattattggt aaatatcctc ctctaagtgt gcaacaaatt attcaacaac aaccacaagc
     4141 aattgtgatt ttaggtggag gtttgtatag agggggcgaa tataataatc aatatcaatc
     4201 gggaggtgca tcaacagcgc gtttacagta tggtgcgtat ttagctaaac aaacacgatt
     4261 gcctattggt ttatcaggaa tggaagtcgc ctatggtatg agcaatacct taggtcaatt
     4321 tggcttagca gcaacttggt tagaaaataa tagctatgat acccatgaaa atgcccaatt
     4381 ttcagcacaa ttattaatta agaacaatat acaaaaaatt gtcttagtta ctgatgcttg
     4441 gcatatgtcg cgttctgttt tagcttttag gcaagctgga tttaatgtaa ttcctgcacc
     4501 tacagagttt cctgatggtt tttatattaa taaaaaacaa tggtggcaag ccaacttaaa
     4561 tacctatatg caaaacttac gtggctggac agaaattttt ggccatgtta aatatcgggt
     4621 tcgttatgcg ttgtttagca aagcctgatc taaggtaggg cttttaaaat caaagtctaa
     4681 attaattaat cgttgtggaa tcactggttg tccatctaat aacaacgttg acatttctcc
     4741 taataataat tttagaacaa tgctaggcac aggaaagggc gcaattctgt gtaaatgttg
     4801 ggcaaaaatt tgggtgaatt gttgattagt cacaggatga ggtgcggtta aattaaaaat
     4861 accgtcaact tgtggatgat gaattaaaaa taaaatagca gcaataaaat catctaggcc
     4921 aatccatgaa aaccattgtt ggccattgcc caaacgtccg cctaaattta atttaaaaac
     4981 aggtaataag cgttttaaca taccgccttg agcagctaaa accacacctg tacgaataat
     5041 acaaacacgt gtactagggc tttgcattgt tagggcggct tgttcccacg catggcataa
     5101 gtcgtgaaca aattcttgat gcggagctga gttttcatca actctattat tattttgatt
     5161 accataccaa ccaattgctg agccagaaat taacagttgt ggttgatgcg agcattgttt
     5221 tagccaattt accaaatctt tagtcagact aacacggcta tcatacaaag cttgtttacg
     5281 tttatttgtc cacggtttgt cggcaatccc ttcacctgct aaattaatta ccacatcaaa
     5341 ttgactattt gggtttaagt gttcaatttg gttgactgct tgtactttgc cttgatattg
     5401 cttagtaact ttgtcaggct ggcgagtaaa aatggttaat tgatgttgtt gtttgagcaa
     5461 ctctgcacat aaagcttgtc ctaaaaagcc agtcccaccc gtcagtaaaa tacgcatgat
     5521 gtcctcatct acaaaaaaca gattaatttt tgtaagttaa cagtatcttg ttaactgtca
     5581 atcaaacaaa aatggctcat taatgtttta ttatgactta cgcggttatc gcttatttgt
     5641 tcgcatttca tgcgttttta acgcctttat gctcacaaaa cgcgctaatt tcggtgaaat
     5701 gcataagtcc tatttgcttt tgtaatgtta taatataaca tttatgaaat tgctaatgaa
     5761 tgagaaaaag tcatgttacc tgttaccgtt ttatctggat ttttaggcgc aggcaaaacc
     5821 accgttttgc agcatatttt gcataatcgc caaggtttaa aagttgcctt aatcgtcaat
     5881 gatatgagtg aaatcaacat tgatgcggcc ttggttgcag gtgaggccag tttaaattat
     5941 caacaagaaa aattggttga aatgtctaat ggctgtattt gctgcacttt gcgcgaagat
     6001 ttgttaattg aagtcagtga attagctaaa tcgggaaagt ttgattattt agttattgaa
     6061 tcaacaggta tttctgagcc attaccaatt gccgaaacat ttacctttag agatgaacag
     6121 ggtgtgtctt tatcagatat tgctcgatta gataccatgg tcacagtcgt tgatgccgtc
     6181 aattttacta aagattattt tgctgcacag tctttggctg agatggacga aaacttgggc
     6241 gaagatgacg aacgaacctt gagtgattta ttggtcgaac aagttgaatt tgccgatgta
     6301 attttggttt ctaaaatcga cttagtttct agtcaggaag tggaaaattt attagcggta
     6361 ttaagaactt taaacacaca agcgaaaatt tatccaattc aacaaggtca agttgactta
     6421 gaaaaaatta ttaataccca attatttagc tttgaacaag cccaacaagc cgcaggatgg
     6481 ctaaaagaaa tgcgtggtga acatattccc gaaagtgaag aatatggcat tgcatcagtc
     6541 aattttgtgg caagaagacc gtttcatcca caacgatttt ggaacttttt gtatcaagat
     6601 tggcaacaag aacaaggccg cttattacgc gctaaaggct acttttggtt atcatctcgc
     6661 ttaaaaatgg ctggtgtgat gtcacaagca ggaggcgtgt tacgacatgg tggtgcagga
     6721 tactggtggg cggctgtgcc agaaaccgaa tggccaacag aacaagatta tctagccaga
     6781 attaatgaaa aatgggatga agaagtgggt gattgtcgac aagagttagt ctttattggt
     6841 caaaatttgc caagtgatat attgttaaaa caattacata cttgtttgtt aactgaggaa
     6901 gaaatggcag aaggaattga atattgcgaa aaattgccag acccatttcc tgaatggtga
     6961 taactggtta atatgcgcct ctgataacaa tatggacaat aatttttatc atgcgcattt
     7021 tatttattca tcaaaacttt cctgctcaat ttaagcatct tgcaccagca ttggcggctg
     7081 ataaaaacaa tcaagtacta gcaatgcata tgcgctctga tatgccaaaa aattggcaag
     7141 gtgtgcaaat gttatcctat gctgccaagc gcggttcaac gccaaaaatt catccttgga
     7201 taacagatat agaaaccaaa gtgattagag gtgaagcagc tttttatgct gctatgcagt
     7261 taaaaaaagc aggatttacg cccgatgtga ttattgccca ttgtggatgg ggcgaaagtt
     7321 tatttgttaa agatgtgtgg ccaactgcta agttagcgat ttatagtgag ttttattatc
     7381 atgctcaagg cgcagatgta ggctttgacc ctgagtttcc ttcatcttta accgatcaat
     7441 gtcgaattag agtcaaaaat actaataatt tattgcactt tgaagtggct gatgcaggca
     7501 tttctcccac ttattggcaa gctagtacct ttccacagcc gttcagagat aaaatcactg
     7561 tggttcatga cgggattgat actcaagcga ttaaacccaa tccaacgata caaatgaatt
     7621 ttaagaatgc tcagggtgag attacgctca gtcgcaacga tgaagtgatt acctttgttg
     7681 cgcgtaattt agagccttat cggggttatc atatttttat gcgtgctttg cccgatattt
     7741 tgcgccaacg acccacagca aaagtattaa ttattggtgc agatggggtg agctacggta
     7801 aagaagcacc acaaggccaa acatggaaga atatctttat taatgaagtt aaagagcaag
     7861 tggattggtc tagggttttc tttttaggcc aagtgccgta tccacaattt attnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncaaaatg cccgccaatt cgctgttgag
     8041 aattatgatc taaaaagcat ttgtttgccc aaacaattgc aatgggttca aagcctagtt
     8101 aattagagag agcaattatg cagcctattt tatcggctat tcccaccaac atcatcacag
     8161 gttttttagg ctcaggtaaa accaccttaa ttcaacaact attggcaagc aaacccgaaa
     8221 gtgaacgttg ggcggtgttg gtcaatgagt ttggtcagat aggtatagat caagcgttaa
     8281 tcgggcaaca gcaagaagta tttattaaag aagtggcagg tggttgtatt tgttgtgcca
     8341 atttcttgcc gatgcaagtg gcgttatcgc aattactcgc caaagcaaaa ccacaacggc
     8401 tgttaataga acccacaggt ttaggacatc ccaaacaaat tattgatagt ttaagtgcta
     8461 gtcattggca aagcacttta aaacttgaag cagtcttgtg tttgattgat gcccgacaat
     8521 atcaagacca acgagtgcgt gaacatgagt catttattgc tcaagtagag gttgcggatg
     8581 ttttggtgtt tagtaaagcg gatctgttaa atgtagagca aaaacaatta atgttagatt
     8641 ttgcacagca attaccatca cccaatgcaa aaacggtatt tgttgagcac ggccaattag
     8701 atcatgagtt attaggctat aaacgccaac aaattcctgt acttaaacgc tctttattgc
     8761 acgataattt acgcttaaat gccgttcaaa atacagcatc aacagacatt gttgaaccac
     8821 catatcatta tcaccaacaa gccttagaca cctatgttgg tggttggatt tttcctgtgg
     8881 attggcaatt tgaacaccat gcgttaatgg atttattatt tgcgattaag gcacaacgcg
     8941 taaagggagt tattcatact aatcaaggtt ggttggcagt gaatttaacg gctgttgatg
     9001 ccagttttaa tagttgtgcc gagtccttcg atagtcgttt agagattatt agtgcaaata
     9061 atatagattg gtcggcatta gaacaggcat tgttagcgat tcgttctaat cctacgcatt
     9121 gttaataatg cgtaggaaaa gagggcaaac taagcaaaca aatccatttg ctcgccagct
     9181 ttgatttttt gtttggcttt tcggttgtgc caccattgct gagcaagggt taaagcttct
     9241 tctggatcat tacaacgttg taggccaatt aatgccagtg ctaatgtgcc aatcactgct
     9301 gattcaccat actcatgtgc tttttggcca cgccataccg caataaaatc gtccaaacta
     9361 aattcatctt cagcattatg acgctcttca aacatcatcg gccattctaa atcgtaaagc
     9421 tcgccgtttt ttatgccttt aaccaaacaa cgcccatcag gattacgctc aaattcgccg
     9481 ccttcacctt ttatcaccat cgagttgctg taatttaata atttagccgc ttgttgatga
     9541 gattcacgat atgcaggatg aaaaatcgct tgtaatgaac atttggcatt taaggggtta
     9601 attaaacgcg ctaaggtatg tactggcgaa cgtaaaccca taatgttacg taattgaata
     9661 atattgccta aaggagggca gtaagcgtcc aaagacatat aagcaaagtt tttttgatta
     9721 agctgcagct ctacatctgc ccatgtttga cacaaaccat aacccaagac aggtaacact
     9781 tcttcggtgt ataaacgatt aatggtatga ccactagcac cgtgcataaa aatgcgcacg
     9841 ccattttcgg ctaatactaa agcagcaagc aaaaaccacg gataatgttt gcgtttgcct
     9901 gcataagatg accagtccaa atcaacggca atgtgtgggg cttgtagcca ttcgcgtgtg
     9961 gctgccacaa aacctgccag ttcttcgggc gattcttctt ttacccgtaa caacatcaaa
    10021 aatgcgccaa gttgcgcttc taacacttcg ccacgcacaa tcatcgcaaa cgcggcttgt
    10081 gcttcttcaa agcttaatga gcgactacct gttttgcctt tgccaatgat gcgaatatat
    10141 tgcgcaaaag ggtgttcaac atcacgatag atgtttttca taaccatgtt gttaaaatcc
    10201 taaatacagt tggtgggttt gggtaaccct gctaagcgag caatcgtttt ggcgggagta
    10261 ccatcaccaa aaagagacat cacataaatg ctactgcttt tttcgctacc taattgttgc
    10321 cctaaatatt ttaataatac ccttgtggtg ggtgagcgtt ggtattgctg ataaaagtct
    10381 ctaatagcat aaagaatttg ccagtgttcg tcatgcaaaa ttaattgctg ttgttgtgcc
    10441 agttgtgtgg caatgtcagg tgtccaatcg tgatgattaa gcaaatagcc ttcttcatca
    10501 acaccaatat cagtgccagc taatactgcg ctcatatagt attcctaatt gcacaaattc
    10561 aggatagtca attattttac aagaagtttg ctcaactaag gttaataaac cataagcttg
    10621 taagtcactc attaaggcat aacaacgata cttttttaat aaatcctgct gagaatataa
    10681 attatacact ccctgatttg tgaataataa aacatcatca tcagataaaa tagtaataag
    10741 ttgctgataa agttcgttac taagtgtggt actaattaaa tgaagggtag gcatgattta
    10801 gagtcgaata atagggctgt gttgctgaaa aaaatctttt aaggattctt gtgaaagtat
    10861 ttgtacgtca atagcgaggt gttcggcttt taaattgcgt gttaataagc tttgctcatc
    10921 aacatacacc tccgaaacgt catagagttc taaggcactg agttgggcaa taattgaggc
    10981 tttgccaaca atttcacttt gttgatttgc caatagttgc caaacaccat caccctgaaa
    11041 taagacactg ggattttggc caaatgcagc cattaccaat aaggcatcta aactttcttg
    11101 agcatggtgg ccgtgataag gggcggtatg atgaataaat aatggtttca tgttaaaact
    11161 gtaaaaatct atcggagtta atcattaaat cggtcaacgt gcctaaacct gttaaggtaa
    11221 agccatcggc taagttatca ccttgtaagt ggtggcggtg gctattatcg gcatcggtga
    11281 tgccacggcg taatgcggca gcgacacaaa cggctaattc taattgatgt tgtttggcca
    11341 aattttgcca acattgattt agtgaatttg cgttatcatg gttcagtatc aaacgagtgg
    11401 caactgtcac gccatcatta taaaaaaaag gcgatacctg atgaccttgc tccaatgccg
    11461 ttttggcgaa ataataagca tgccatgcgt tgctactctc aattggggcg gtaattaaca
    11521 agacaaattt cataaaatag tattcaatcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11641 nnnnnnnnna agtcataaat catataacag gacttattat gcagccatat ttattaagtt
    11701 tagatcaagg cacaaccagt acccgtgcga ttatttttaa tttacaaggg caggctgttg
    11761 ctcgcgcaca actagaactc aaacaatatt ttcctgctga tggctgggta gagcatgatg
    11821 ctgaagaaat tgcccaatcc tctttagcag tgtgtcgcga agctttacag caaagtggta
    11881 ttactgcaca acaaattgca gccataggta ttactaacca acgtgaaaca acagtcgttt
    11941 gggatcgaaa aacaggcaag cctattcaca aagcgattgt ttggcaagat cgccgtactg
    12001 ctgatacttg tttaaaactt aaaaatcgtg gccatgaaac aatggttcag caaaaaacgg
    12061 gcttgttatt agacccttat ttttcggcca gtaaattagc atggattttg gataacgtct
    12121 caggcgcaag aaaacgcgct gaaaagggcg agttggcttt tggtacaatt gatacatggt
    12181 taatttggca actcacaggt ggtaaaaaac acgctaccga tgctaccaat gctgcacgta
    12241 ctttattatt taatattcat acccaagaat gggatgaaga attattaaag ctatttaata
    12301 ttcccgtcag tttattacct gttgtccaag attctgccag tgattttggt ttaacggaaa
    12361 cgcgtttatt tggtgcgcct atgcaaattg gtggtgtggc aggtgaccaa caagcggctt
    12421 tagtggggca agcgtgtttt aactctggta tggtcaaaag cacctacggc acaggctgtt
    12481 ttatggtgat gaatacaggc gatgaagcca ttaactcgac tcatcgtttg ttaaccaccg
    12541 ttgcctatcg tttaaatggt aaagtgacgt atgccttaga aggcagtatt tttgtcgctg
    12601 gcgcagccgt acaatggttg cgtgatggta ttcaattgat taaacaagct ggcgataccg
    12661 aagccattgc cgaagaaacc atggatagcc gaggcgtgta tttagtgcct gcgtttacag
    12721 gtttaggggc gccgtattgg gatcctcatg cacgaggcgc aatttttggt ttaacacgtg
    12781 atacaggcat tggcgaaatt gttattgctg gcttagaggc tgtttgtttt caaacgcgcg
    12841 acttgatgga agcgatgaaa aaagatggcg caaccaaatt aaaaactttg cgtgtagatg
    12901 gtggcatggc agttaataat tggctgatgc aagctttatc cgatattgtt ggggtggcgg
    12961 tggatcgtcc aacagttact gaaacaacgg ctttgggtgt ggcatggtta gcaggcttac
    13021 aggttggtaa atttgcatct ttggatgcca ttgctgagtt gtggcattgc caacgtgaat
    13081 ttacccctaa aatggatatt gataaacgcg aaaccatgta tgcaggttgg ttagatgctg
    13141 tgcaaagagt caccttaaaa accgtgtaat tcttaaaaaa agaggctacc attaatagga
    13201 cttacacatt gcagtgcaat tagcgcgttt tgtgagcata aaggcattga taaatccctg
    13261 aaatgtaaac aaatacgcga taatcgtgta agtcgaaaag agtttctttt ttattatatt
    13321 cttatacact ccctataatg gtgaaaaatg attttactta caggtggggc aggttatata
    13381 ggttctcaca ccgcgttaga actacttgcc gcaggatttg acattttagt tttggataat
    13441 ttaagtaata gcaaattaga ggccattaac agaacacgtc aactagccaa taaaaatttt
    13501 gaatttattg aaggggatat tcgagatcgt caattgttag ataaaatctt taagagttat
    13561 ccaattacag ccgttgtgca ttttgcaggc ttaaaagcgg ttggtgaatc tgtgcaaaaa
    13621 ccgctttatt attatgataa taatgtcagt ggttcggttg tgttatttga agcgatgaca
    13681 gccgctaata tcaaaaccat cgtatttagt tcgtctgcaa ccgtgtatgg tgatcctcat
    13741 actgtaccta ttaaagaaga ttttccgtta tcaacgacta atccttatgg agcgagtaag
    13801 ttgatgattg aaaatatttt acgcgatgtc aaagtcgccg ataacagttg gcgaattgca
    13861 ttattacgtt attttaatcc aactggcgca catgaaagtg gtttaattgg tgaagaccct
    13921 aatggtattc ctaataattt gatgccatat attgctcaag ttgcggtggg taaacgtagt
    13981 catttacaag tgtttggtgg tgattatcca acgcatgacg gtactggcgt gcgagattat
    14041 attcatgttg ttgatttagc taagggacat gttaaagctt tacaatatct tgaacaaaat
    14101 caagaattag tcaccgttaa tttaggaaca ggtaaaggtt actcggtatt agatatgctt
    14161 aaagcaatgg aaaaagcagc aggaaaaacc atcgcttatc aaattgtaga acgtagagca
    14221 ggggacattg ccagttgtta tgccgatcca cagtacgcat ttaataaaat tggctggcaa
    14281 gctgaatttg accaagagcg gatgtgtagc gatatgtggc gttggca
//
LOCUS       DAOQMB010000017        14266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1561_length_14266_cov_15.681866, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000017 DAOQMB010000000
VERSION     DAOQMB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 14266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14266
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1561_length_14266_cov_15.681866"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..157
                     /locus_tag="PLY05_01440"
     CDS             <1..157
                     /locus_tag="PLY05_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009387635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HNC01994.1"
                     /translation="QGTRTAMRAKAYPAEDPSQVPPAEHIMPLFLFLMGAESVGVNGK
                     SFDARDK"
     gene            complement(208..621)
                     /locus_tag="PLY05_01445"
     CDS             complement(208..621)
                     /locus_tag="PLY05_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC01995.1"
                     /translation="MKKLALLAALVGLSCSLNTYAKEDQSAVIERIKPYGSVCVQGKE
                     CSVKAPVAAAAAPAGGEPRSGEQVYTAVCAGCHGAGVMGAPKYGTADWAPRKAKGMAT
                     LYDHAIKGFNSMPAKGGCAACPDEEIKAAVDYMAK"
     gene            1070..5809
                     /locus_tag="PLY05_01450"
     CDS             1070..5809
                     /locus_tag="PLY05_01450"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HNC01996.1"
                     /translation="MLSSTEAWSQQTRQNCLDVCAAYENPLFATFAERALKALLGDEL
                     RRYTPHSLSACLWGFWQFVADSRQEINIRVFNPEPVKAGWRSEGTVIEIYSSEMPFLL
                     NTLRIALQRGGYDVSVLMDINLPLRRNRYCQITDIPKNTNANADEYRSLIHIELSEVF
                     ENDALKDLETHIYASLNMLRRVVDGFPRMQGLLRDAAQKLIEKMPKEGDAAVIRMESH
                     AFLQWLIRGHFTFLAVRHRDESGKEEIAGIDPISTNLHIDWLDDTFPNTIKQEKEGRL
                     FFGKSLTRSPIHHDRYADVVIVPMPDGKSEYRFWGLYTSRVNQHDPMTMPVLRHKITT
                     VDISSGLRKISHDGRNFDRMLRTHPRDELLLATEEDLLNAFLPMVKQKYGNELRFIWR
                     LDPWKRFISVFIYLPKPLYNQTFVSRTGEFLQARFNATDAVMTAYVSEHRWIRLQGLL
                     VFEDKNPPRINIEETESVLKRLARTWDDELLSLLMTKYQPMLANQLFRRYQNVFPEGY
                     KENYRPQDALVDVTFLETLRPENPLAVEVSPTEGRTARFKILQWHQQLSLSRVMPILE
                     SFGLKVLQEQAFPLLLESNCLWLHDFRTELPDNLAREAFVQTLAYVREAMQVLWQGAI
                     EADNFNRLVTTLPCRWREAHIFRVLSAYLKQTGFPLSATAQVEALTRYPNIGRSLLEL
                     FQTRFDPSIITDRSERQDAILKRIDLALDKVSSLSDDRVLREFMALIMAIVRTSYYMP
                     SAIKSQRIAIKFDSSQLRNLPNPKPWREVFVYSPDVIGLHLRFGQVSRGGIRWSDRPE
                     DFRTEVLGLVKAQQVKNAVIVPVGAKGAFVVRATYGDKYDIALNAYQEFLRGLLDITD
                     NRHKDKIISPKNVVCHDGQDTYLVVAADKGTATFSDTANAVAAEYQFWLGDAFASGGS
                     NGYSHKAMGITARGAFISLARLAKEADFDPYNEPFSMVGIGDMSGDVFGNGLLLSKQI
                     RLQAAFNHNYIFIDPYPDPEISYQERKRLFALPQSDWRDYNRELMSEGGGIFSRNERK
                     IVLSEEMKDMLMVQDNILSADDLVRAILKMPVDVLWNGGIGTYVKASDENHADVGDRL
                     NDNVRVNANQLRARIVCEGGNLGFTQRARIEYQLNGGLSHTDFIDNSAGVDCSDHEVN
                     IKIFLQREQQARRLSADDYKKLLAEWRDEIAQAVLNNNYAQTQCLAIANHQSLLRHRE
                     YSTLADFLEVNAGLDRHLEAIPDVRIWEARIRTQQPLTRAELAILLAYTKNWLKAALS
                     TAELFNDKQLMTEAQALFPVAIRKNYASTLQKHPLAVSLAATRLARLMTERLGMGSIP
                     RLLQNERSTPLKLARAFMVARALLGLDQVWQQLDDSKDLADSVLLPQYAALQSYTRRA
                     CGWFLAYAEKPSEQIIADFSMLAPLLNDLPNHLNHKRLEGWQNAKNDLVAKGFNAHLA
                     ERLASLDQILPLLDMCRLAQDKGISVENVAYFYEVIEETLGLTDVQRALQNLAVNNTW
                     EAAARDQLRAGLLRDMAQLTEDALSKWKSTQESQQSWLTMWLRANQQIFIDWHQFRRR
                     LSPSDLASYAPYVVLTSQLHKLLIQLK"
     gene            complement(5840..6361)
                     /locus_tag="PLY05_01455"
     CDS             complement(5840..6361)
                     /locus_tag="PLY05_01455"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNC01997.1"
                     /translation="MRYLALLAVMLCNTAWAEFTPKTVSKVDLNRYMGKWYEVAKIPN
                     TFQKSCVQDITASYSLLANNQVKGINSCRKANGQIFQMGIQGKVKDNSNAKLGFKITS
                     SWLRFIPMLETDYWIVDLAEDYSHATVSTSDGKYLWILARQPQLAEPVYQQILQRVAK
                     LGFATDKVVKNKS"
     gene            complement(6371..6616)
                     /locus_tag="PLY05_01460"
     CDS             complement(6371..6616)
                     /locus_tag="PLY05_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03643 family protein"
                     /protein_id="HNC01998.1"
                     /translation="MQAQDLSRIIEMAWEDRTPFEAIQQLYGLNESAVIRLMRKTLNP
                     NSFKLWRKRVTGRKTKHLALRGFESGRAYCPQQYKQH"
     gene            complement(6777..8018)
                     /locus_tag="PLY05_01465"
     CDS             complement(6777..8018)
                     /locus_tag="PLY05_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004958716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HNC01999.1"
                     /translation="MSDLSIPHIAIIGGGAAGLMAADILRAYPVQVSIYEAKPSVGRK
                     FLLAGKGGMNITHSEDFDAFCQRYGAEKPFLQPMLEQFNNQAVRDWMQGLGFDSFVGS
                     SGRVFPTDMKAAPLLRAWLHRLRESGIKICVRHRWLGWTNESKLKFATPEGEQHIAAD
                     AVILACGGGSWPQLGSDGAWQSVLQAQGISVKPLQAANCGFDSQWSSNFSQEFAGQPI
                     KSAVLAFTDAQGNAYRKQGDFVITATGIEGSLIYALSRPLREFINQNGKVTVYLDLFP
                     HKTVEQLYTLLNKPRAASLSWSSFVRKQLGIQGVKAALLRECTTKQQMQDVKQLASLL
                     KVLPITLTQTRPIAEAISSAGGVKWSALTADLQLKNRPKVFCAGEMIDWEAPTGGYLL
                     TACFATAKCAALGVVRELGSN"
     gene            8113..9114
                     /gene="dusA"
                     /locus_tag="PLY05_01470"
     CDS             8113..9114
                     /gene="dusA"
                     /locus_tag="PLY05_01470"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004794629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HNC02000.1"
                     /translation="MTLDLSPLPSPKILSVAPMMDWTDRHCRYFLRLFNRHVQLYTEM
                     VTTGALLHADKHRFLRFAEQEHPVILQLGGADPTDLARCSQFAEDYGYDAVNLNVGCP
                     SDRVQKNKIGACLMAEPNLVADCISTMQAAVKIPVTIKHRIGIDDLNSYENLYKFVET
                     VAQTGCQTFIVHARIAILQGLSPKENREIPPLRYQDVYQLKKDFPHLDIQINGGIKTV
                     DDIQQHWQHTDGVMIGREAYHNPYILAQALKFWGEDTPTPHQILENFLPFMEQELANG
                     VPLMQMTRHILGLFQGMTGARKWRQHLSTYAHQKHHGIELVKEAASFVQANTLLEPV"
     assembly_gap    9325..9424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9439..10389
                     /gene="tal"
                     /locus_tag="PLY05_01475"
     CDS             9439..10389
                     /gene="tal"
                     /locus_tag="PLY05_01475"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HNC02001.1"
                     /translation="MNQLEQLKKLSTIVADTGDIASIQRLKPVDATTNPSLILKAANQ
                     AEYAHIIDAGIKEAQKLPTEQARLQAAIEAIGVGFGTEILKSIDGVVSTEVDARASFD
                     TKATIEQARRLIQRYQQQGIGRERVLVKIASTWEGIKAAELLEQEGIHCNLTLLFSFA
                     QAQACAEAGVTLISPFVGRILDWYKKSTGQDYTADTDPGVVSVKRIYEYYKACDYKTI
                     VMAASFRNTGEILALAGCDKLTISPALLDSLSAESSPISCQLNSQNITHQTCLTRLGD
                     AEFRWQHNEDAMATEKLAEGIRLFAADIRLLEQKISKLLG"
     gene            10562..>11262
                     /locus_tag="PLY05_01480"
     CDS             10562..>11262
                     /locus_tag="PLY05_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE2 domain-containing protein"
                     /protein_id="HNC02002.1"
                     /translation="MRLLAYMVAVAIFASGIYTGIYHPKMTAMLPVVLALAFTQSWLH
                     RHLRNSIPSQATAVTLLLDGLITGFGIAAVHYALVPMLVFIAMLNATVLGFGSLFLWV
                     FTGFFCVLGSLLGGFAFGLEPNVHESIPMSVNAVALVGLAVYFGLNSFHSRAQSRDLI
                     TAKQELQEQKEESFQLSRKLIKYLPPQVWESIFKGKRDAKLQTQRKKLTIFFSDIKDF
                     TETSDEMPPDLLTEML"
     assembly_gap    11263..11362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11363..11758
                     /locus_tag="PLY05_01485"
     CDS             <11363..11758
                     /locus_tag="PLY05_01485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC02003.1"
                     /translation="NTGYCHVGNFGSETRMSYTIIGRDANLAARLQSIAAVDEILISH
                     ETYLLIRDQILCKEKGAVTLRGIAKPTQVWEVVDFHKDINSHSSWIEHELSGFTMHMD
                     LSKIRNFEKEAVLKALEQAEKKLRDTKIL"
     gene            11858..13042
                     /locus_tag="PLY05_01490"
     CDS             11858..13042
                     /locus_tag="PLY05_01490"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC02004.1"
                     /translation="MNKNLIATLLLGAVLGASGNYYLQQRPIKVTLIKPQISQLQSTI
                     LATGQVKNSSHTTLHNENAGLINQVLEEGTVVSKGQTIAFIQDKEIEPLLLQQQANVK
                     NAQLKLNRLQTIERQQAELKVEQARILLKQAKRQLSDTQALAQQQLTSQNNLITAQET
                     LSLREKELATALLQQQAVQAQGLDEQTALNQLQQAQALLAQSQIRQQRQTIISPFDGV
                     ITERKVSVGQYVKQGEAIVALSPKQAAEIIANVDERWLPQLHLKQQASVIADAFPQQA
                     FTAQISYISPTIDNSRGTVEIRLQNTQWPSFLQEGMTVSLELLSQAVQKTVIIPSKLL
                     QQNDNQYWVWLAKNQQAERQNVRIGLRHLDQVQIIEGLDENSQLIDSPTPLQAKQAIQ
                     AH"
     gene            13046..14260
                     /locus_tag="PLY05_01495"
     CDS             13046..14260
                     /locus_tag="PLY05_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC02005.1"
                     /translation="MVWFEWQLATRFLKEGKTQTLLIASGIALGVAVIVFITTLVTGL
                     QRTIIDRTLGTQAHIVLTEPDKELLASVKGEQVFRQTQPRPQRIEPILNWQLIEQKIK
                     TDPQIKAVSPVLSGAAFALRGQADKSVAILGIQPEAYNRIIPLKDYLKQGQLQLNSNE
                     AMIGTRLADDLGIRVGDNLNLLTANNRQISVRITGIFEFGIKDLDRRWVYLSLRSAQN
                     LLDLQGQITGIDLQVYSLFDAEKIAQQLQQQYPIKAESWMSSNQQLMTALRSQTASTT
                     LIRFFVLISVAFGIASVLAIAVVQKQKEIGILRAMGLSQRSVLVVFLIQGGLLGFIGS
                     ILGSLAGMGLVKIFTLFVKSATGEPLFQLALTQEVFVTTMLVATLTGLISAAMPALRA
                     AKLDPVQAIRYG"
BASE COUNT         4274 a         2883 c         2722 g         4187 t
ORIGIN      
        1 tcaaggtact cgtacggcaa tgcgcgccaa agcctatcct gcggaagacc caagccaagt
       61 accacctgcc gaacacatta tgccgctgtt tttgttttta atgggggcag aaagtgtggg
      121 tgttaatggt aaatcgtttg atgccagaga taaataaagg tttgataaaa aaaccctgca
      181 tttgcagggt ttcttttaat acttggctta cttagccata taatcaacag cagctttaat
      241 ttcctcatca ggacaagcag cacaaccacc ttttgcaggc atagaattaa agcctttgat
      301 agcatgatca tataatgtag ccataccttt agctttacgt ggtgcccaat cggctgtgcc
      361 atatttaggc gcacccatca cccctgcacc atgacaccct gcacaaactg cggtataaac
      421 ttgctcacca gaacgaggtt caccaccagc aggagctgcc gcagcagcaa caggtgcttt
      481 aaccgaacat tctttgcctt gtacacacac gctaccataa ggtttaatgc gttcaatcac
      541 tgccgattgg tcttctttag cataagtatt taaagaacag cttaagccaa ctaacgctgc
      601 taacaaagct aattttttca cagaaagcct cacaaaagca taacaattaa aatttgcgcg
      661 caagtataac cgcaaaactc ataaggacaa agtattccct gcaatgacat caaaattatt
      721 tagctcaaat aatcaacaac cctttaagaa ataatacctt ttagacaatt catttggttt
      781 aaatcaatca tgagtcatca taaagtttta cacagtcatc gtttatttgt gcgcatttca
      841 tccgttctat atccataaaa cgcgttacct ttggcaaaat gcgtaagtca tacacatctt
      901 cgcaacaaaa tgttcagtaa gtaatctacg gtcaattgca caattttgcc aagcagtgta
      961 tatcttaaag tttaataaat agctaaattc taaaacaacg attgaacaat aaacttttaa
     1021 ttcgtcttta aaatttaaaa agaattattt ttacaaggag tgcaaactca tgttatccag
     1081 caccgaagct tggtctcaac aaacacgaca aaattgctta gatgtttgtg ctgcttacga
     1141 aaacccttta tttgcgactt ttgctgaacg tgccttaaaa gccttattag gtgacgaatt
     1201 acggcgttat accccccact cattgtctgc gtgtttatgg ggattttggc aatttgttgc
     1261 tgatagccgt caagaaatta acattcgtgt ctttaatccg gagcctgtta aagcaggatg
     1321 gcgctccgaa ggaaccgtta ttgaaattta tagttccgaa atgccttttt tgcttaatac
     1381 tttaagaatt gccttacaac gtggtggcta cgacgttagc gtgctaatgg acattaattt
     1441 accactacgt cgtaatcgct attgtcaaat tacagatatt cccaaaaaca ctaatgcaaa
     1501 tgctgatgaa taccgtagtt taattcatat tgagttatcc gaagtttttg aaaatgacgc
     1561 attaaaagac ttagaaaccc atatttatgc ctcattaaat atgttgcgta gagtggttga
     1621 tggctttcct agaatgcaag gattattgcg tgatgcggca caaaaattaa ttgaaaaaat
     1681 gcctaaagaa ggcgatgcag ccgtcattcg tatggaatcc cacgcttttt tacaatggtt
     1741 aattcgtggc cattttactt ttttagcggt acgtcaccgt gacgaatcag gcaaagaaga
     1801 aattgcaggt attgatccta tcagtactaa cttacatatt gattggctag atgacacttt
     1861 tcccaacact attaaacaag aaaaagaagg tcgtttattt tttggtaaat ctttaactcg
     1921 ctcgcctatt catcatgatc gttatgctga tgtggttatt gtgccaatgc cagatggcaa
     1981 aagtgaatat cgtttttggg gcttatatac cagtcgggtt aatcagcatg acccaatgac
     2041 catgcccgtc ttacgtcaca aaattacgac ggtagatatt tcgtctggat tacgcaaaat
     2101 tagccatgat ggtcgcaact ttgaccgtat gttacgcaca catccacgag acgaactctt
     2161 attagccaca gaggaagatt tgcttaatgc gtttttacct atggtaaaac aaaaatatgg
     2221 taatgagttg cgctttattt ggcgtttaga cccgtggaaa cgctttattt cagtttttat
     2281 ttatttacca aaacccttgt ataatcaaac atttgttagc cgtacaggag aatttttaca
     2341 agcacgtttt aatgccactg atgcagtaat gactgcttat gtttcagaac atcgttggat
     2401 cagactacaa ggtttattag tttttgaaga taaaaaccca ccacgtatta atattgaaga
     2461 aaccgaaagt gttctcaagc gtttggctcg aacatgggat gacgaactgt taagtttact
     2521 catgactaaa tatcaaccaa tgttagccaa ccaactattt cgtcgctatc aaaatgtttt
     2581 tcccgaaggt tataaagaaa actatcggcc tcaagatgct ttagtagatg tgactttttt
     2641 agaaacatta cggccagaaa atcccttagc tgtcgaagta tctcccacag aaggcagaac
     2701 agcacgtttt aaaattctac aatggcatca acaattgtct ttatcaagag ttatgccgat
     2761 tttggaaagt tttggcctta aagtgttaca agagcaagcc tttcctttat tgttagaaag
     2821 caattgttta tggctacatg attttagaac cgaactacct gataatttag cacgagaagc
     2881 ctttgttcaa accttagcct atgtgcgtga agccatgcaa gtattatggc aaggcgcaat
     2941 tgaagcagat aattttaacc gtttagtgac aacactcccc tgtcgttggc gtgaagctca
     3001 catttttaga gtattatctg cctacctaaa acaaacaggt tttccattgt ctgcgactgc
     3061 ccaagttgaa gcactcactc gctatcccaa tattggtcgt agtttattag aattattcca
     3121 aacccgattt gacccaagta ttattacaga tcgctcagaa cgacaagatg caattttaaa
     3181 gcgtattgat ttggccttag ataaagtcag ttctttaagc gatgatcgtg tgttacgtga
     3241 gtttatggcc ttgattatgg ccattgtacg cactagctac tacatgccat cagcaattaa
     3301 aagtcaacgt attgccatta aatttgactc ctcccagtta cgcaatttac ctaacccaaa
     3361 accttggcgc gaagtctttg tctatagccc cgatgtgata ggtttacatc tacgttttgg
     3421 ccaagtgtct cgtggtggta ttcggtggtc agatcgtccc gaagacttca gaactgaagt
     3481 attgggctta gtcaaagcac agcaagtcaa aaatgcggtg attgtccctg ttggtgcaaa
     3541 aggtgccttt gttgttagag ccacttatgg tgataaatat gatattgccc taaatgccta
     3601 tcaagaattt ttacgtggtc ttttagacat tacagataac cgtcataaag ataaaattat
     3661 ttcgcccaaa aatgtcgttt gccatgatgg tcaagacact tatttagtcg ttgccgccga
     3721 taaaggcaca gcaacttttt ctgacactgc taatgcagta gcggctgaat atcaattttg
     3781 gttaggtgat gcttttgctt ctggtggtag taatggttat agtcataaag caatgggtat
     3841 tactgcgcgt ggcgcattta tttctcttgc gcgcttagct aaagaagccg attttgaccc
     3901 atataatgaa ccattcagca tggttggtat tggcgatatg tcgggtgatg tatttggcaa
     3961 tggcttatta ttaagtaaac aaatccgctt acaagcggca tttaaccata attatatttt
     4021 tattgatccc tatcctgatc cagaaatcag ctatcaagaa cgtaaacgtt tatttgcctt
     4081 accccaaagt gattggcgtg attataaccg tgaattgatg tctgaaggtg gtggtatatt
     4141 ttctcgcaat gagcgaaaaa tcgttttaag cgaagaaatg aaagatatgc tcatggtaca
     4201 agacaatatc ttatctgccg atgatttagt gcgtgccatc ttaaaaatgc cagttgatgt
     4261 gttatggaat ggtggaattg gcacttatgt caaagcaagc gatgagaatc atgccgatgt
     4321 tggtgaccgt ttaaatgata atgtgcgtgt taatgctaac caattacgtg cgcgtattgt
     4381 ttgcgaaggc ggcaacttag gctttactca acgtgctcgt attgaatatc agctcaatgg
     4441 tggcttatct catactgact ttattgataa ctcggctggt gtagattgct ctgatcatga
     4501 agtcaatatc aaaatatttt tacagcgcga acagcaagca cgtcgcttgt cagccgacga
     4561 ttacaaaaaa ctattagcag aatggcgaga cgaaattgcc caagcggtac ttaataataa
     4621 ctacgctcaa acacaatgtt tagcgattgc taatcatcaa tctttattga gacatcgtga
     4681 atactcaaca ttagcagact ttttagaagt gaatgcaggc ttagatcgtc atttagaagc
     4741 cattcctgat gtacgtattt gggaagcacg tattcgcact cagcaacctt taacacgtgc
     4801 cgaattggca attttattgg cctacaccaa aaattggctt aaagctgctc ttagcacagc
     4861 agaattattt aatgacaaac agttaatgac tgaggctcag gcgttatttc ctgttgcgat
     4921 tcgcaaaaat tatgctagca ccttacaaaa gcatccatta gctgtgtcat tagcggcaac
     4981 tcgtttagcc cgtttgatga ccgaacgctt aggtatgggg tctattccac gtttactaca
     5041 aaatgagcgg agtacgcctt taaagttagc acgtgctttt atggtagcta gagctttatt
     5101 aggtttagat caagtatggc aacaacttga tgatagcaaa gacttagcag acagcgtttt
     5161 actgccacag tatgctgcat tacaaagcta tactcgccgc gcttgtggtt ggtttttagc
     5221 ttatgccgaa aaaccaagtg aacaaattat tgctgacttt agcatgttag cccccttact
     5281 taatgacttg cctaatcatc ttaaccataa acgtttagaa ggttggcaaa atgccaaaaa
     5341 tgatttagtc gctaaaggct ttaatgctca tttagctgag cgtttagcct ctttagatca
     5401 aattttgcct ttattagata tgtgtcgttt ggcgcaagat aaaggaatct cggtagaaaa
     5461 tgttgcttac ttttatgagg ttattgaaga aacattgggt ttaactgatg tgcaacgtgc
     5521 tttgcaaaat ctagcagtta ataatacatg ggaagcggct gcacgagatc aattgcgagc
     5581 aggtttatta cgcgacatgg ctcaacttac agaagatgct ctaagcaaat ggaaaagcac
     5641 tcaggagtca caacaatcat ggttaactat gtggttaaga gctaatcaac aaatatttat
     5701 tgattggcat caattccgcc gtcgtctgag tcctagtgat ttagctagct atgcacctta
     5761 tgttgtctta acttcgcaat tacataaact attgatacag ctaaaataat cgtcttcaag
     5821 ccagcctagt gctggcttgt taagatttat ttttaaccac tttatcggtt gcaaagccta
     5881 acttggccac acgttgcaaa atttgttgat agacaggttc ggctagttgt ggctgtctgg
     5941 ctaaaatcca taaatatttg ccatctgagg ttgaaacagt ggcatgacta taatcttctg
     6001 ccaaatctac aatccaataa tccgtttcta acatgggaat aaaacgcagc cagcttgagg
     6061 taattttaaa gcctaatttc gcgttgctgt tatctttgac tttaccttga atacccattt
     6121 gaaaaatttg gccattggct ttacgacaac tattgatgcc ctttacttga ttgttcgcta
     6181 acaaactata actggcggta atatcttgca cgcaggattt ttgaaaggta ttgggaattt
     6241 tggcgacttc gtaccatttg cccatataac ggtttaagtc tacttttgat actgttttag
     6301 gagtaaattc tgcccatgca gtattgcaaa gcatgacagc caataaagca agatagcgca
     6361 tagattactc ctagtgctgc ttgtattgtt gaggacaata cgctcgccca ctttcaaaac
     6421 cacgcaaagc aaggtgtttg gtttttcgac ctgtcactcg tttgcgccat aatttgaagc
     6481 tattgggatt gagtgttttg cgcattaaac gaatgacggc tgattcgttt agaccataaa
     6541 gttgttgaat ggcttcaaag ggcgttcggt cttcccatgc catttcgata atgcgtgata
     6601 ggtcttgagc ttgcatggca tttattccag ttgggtttgt tttatcttaa ataagacgaa
     6661 tcaactggca ataggacaaa gtttaatcag gagcgaattt gtataacgtg gtttgttata
     6721 taatgtgaat ttaaatgatg ggtttcgtac ctcaacccat cctaccttgc tatttattaa
     6781 tttgatccca actccctcac cacacccaac gctgcacatt ttgccgttgc aaaacaagca
     6841 gttagcaaat aaccacctgt aggggcttcc caatcaatca tttcacctgc acaaaacacc
     6901 ttaggccgat tttttagctg caaatctgcc gttaatgccg accactttac gccgcctgca
     6961 ctactaatgg cctcagcaat gggtcgtgtt tgagttaagg taatcggtaa gacttttaac
     7021 aaactggcca actgtttgac atcttgcatt tgctgcttag tcgtacactc acgtaataat
     7081 gctgctttta ccccctgaat gcctaattgt ttacgcacaa aactcgacca cgacaacgaa
     7141 gcggctcgtg gcttgtttaa caaggtataa agttgctcaa ccgttttatg gggaaataaa
     7201 tctaaataaa ccgtgacttt gccattttga ttgataaatt cgcgtaatgg ccgcgacaag
     7261 gcataaatca aactaccttc aatgcctgtt gcggtaatga caaaatcgcc ttgtttgcga
     7321 taggcattac cttgtgcatc tgtaaacgct aaaactgcag atttaatggg ttgccctgca
     7381 aactcttgac taaagttgct tgaccattgg ctatcaaaac cacaattggc cgcttgtaat
     7441 ggctttacac taatgccttg tgcttgtaac accgactgcc atgcgccatc agagcctaat
     7501 tgtggccaac tgccaccacc acacgccaaa atcaccgcat cagcagcaat gtgttgttca
     7561 ccttctggtg tagcaaactt tagcttgctt tcgtttgtcc accccaacca acgatgacga
     7621 acacaaattt taatgccaga ctcacgcaat cgatgtaacc atgcccgcaa caacggtgca
     7681 gccttcatat cggtaggaaa cactcggcca gaactaccaa caaaactgtc aaaacctaaa
     7741 ccctgcatcc aatctcgtac cgcttgatta ttaaattgct caagcatcgg ttggagaaat
     7801 ggcttttctg caccataacg ctgacaaaac gcgtcaaagt cttcggaatg agtgatgttc
     7861 ataccgcctt tgcctgctaa cagaaactta cggccaaccg agggcttcgc ttcataaata
     7921 gagacctgaa caggataggc acgcaatata tcagctgcca tcaggccagc cgcaccgcca
     7981 ccaataatgg caatatgagg aatagagaga tcagacatga gacaataata atcacaggct
     8041 ctaaataaaa ggtgattaaa acatttaatc agctaaaata tcaaacttta aaatattcat
     8101 tgtttttgtg tcatgacctt agatttatct ccgcttccat cacccaagat tctatccgtt
     8161 gccccgatga tggattggac agaccgccat tgccgttatt ttttacgatt atttaatcgt
     8221 catgttcaac tctatactga aatggtgaca actggtgctt tattgcatgc agataaacac
     8281 cgctttttac gttttgccga acaagaacac cccgttattt tacaattagg tggcgcagat
     8341 cccaccgatt tagcacgttg cagtcaattt gccgaagatt atggttatga tgcagttaac
     8401 ttaaatgttg gttgtccatc tgaccgtgta cagaaaaaca agattggtgc ttgtttaatg
     8461 gccgagccaa atttggttgc tgattgtatt agcaccatgc aagcagccgt caaaatccct
     8521 gtcaccatca aacatcgtat tggcattgat gatttaaata gctacgaaaa cctatataaa
     8581 tttgtcgaaa ccgttgctca aactggctgc caaacattta ttgttcatgc acgtattgcc
     8641 attctgcagg gcttatctcc taaagaaaat cgagaaattc cgcctttacg ttatcaagat
     8701 gtttatcagt taaaaaaaga ttttccgcat ttagatattc aaatcaatgg tggcattaaa
     8761 accgttgatg acattcaaca acattggcaa cacactgatg gagtgatgat tggtcgtgag
     8821 gcttatcata atccctatat tttggcgcaa gctttgaaat tttggggtga agatacacct
     8881 accccccatc aaattttaga aaatttttta ccttttatgg aacaagagtt agcaaatggt
     8941 gtacctttaa tgcaaatgac cagacatatt ttagggttat ttcaaggtat gacaggcgca
     9001 cgtaaatggc gacaacattt aagcacctat gctcaccaaa aacatcatgg catagagctt
     9061 gtcaaagaag ccgctagttt tgttcaagca aacactctcc ttgagcctgt ttagctatag
     9121 acaaaatact gttatattcg atgagtacta ttataaattt actcgtagac aaaacgtagt
     9181 cgaattatct aacgatggtt ggtttcgact acgctcaacc accaagaaga cactcaccat
     9241 aggtgagatt tgcattacat ttaatcaaga ttagggaaca ccaatgaatc aattagaaca
     9301 gttaaaaaaa ttaagtacta tcgtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnattagg gaacaccaat gaatcaatta gaacagttaa aaaaattaag tactatcgtt
     9481 gctgatacag gagatattgc cagcattcaa cgtcttaagc ctgttgatgc caccacaaat
     9541 ccatccttaa ttttaaaagc cgccaatcaa gccgaatatg cccatattat tgacgcaggc
     9601 attaaagaag ctcaaaaact tcccaccgaa caagcccgcc ttcaagcagc aattgaagca
     9661 attggtgtcg gttttggcac agagatttta aaatcgattg atggtgtggt ttcgaccgaa
     9721 gtggatgctc gtgcttcttt tgacaccaaa gccacgattg aacaagctcg tcgtttgatt
     9781 caacgttatc aacaacaagg cattggccgt gagcgggtct tagtaaaaat tgcaagcaca
     9841 tgggaaggca ttaaagccgc tgaattatta gaacaagaag gcattcattg taacttaacc
     9901 ttattgttta gttttgctca agctcaagcc tgtgctgaag caggtgttac cctcatttcg
     9961 ccgtttgttg gccgtatttt agattggtat aaaaaatcaa caggccaaga ctacacagcc
    10021 gacactgatc caggtgtagt atcggttaaa cgtatttatg aatattataa agcctgtgac
    10081 tataaaacca ttgtaatggc ggctagcttt agaaatacag gtgaaatttt agcattagcg
    10141 ggttgtgata aattaaccat cagccctgct ctattagaca gcctatctgc cgaaagttcg
    10201 cccatttctt gccaactgaa cagccaaaac attacacacc aaacttgttt aacacgctta
    10261 ggtgatgctg agtttagatg gcaacacaac gaagatgcaa tggccaccga aaaactagca
    10321 gaagggattc gtttgttcgc tgcagatatt agattattag aacaaaaaat ctcaaagctt
    10381 ttgggctaaa ttctatacat tatgtgtaac tttgttaata accgcttgat tattaacaaa
    10441 gctatgttta tcttgttcag gatattctaa tcataataca aattaaattt tattaagctt
    10501 aatcacaatg agtaacacca acaaccagct acaaaactta ctagagctag gctcgcgaca
    10561 tatgcgccta ttagcctata tggtagctgt tgcaattttt gcatctggta tctatacagg
    10621 catctatcat cctaagatga ctgcaatgtt gccagttgtg cttgctttag cctttacaca
    10681 atcttggcta catcgtcatt tacgaaacag tattccatca caagcaacgg cggtgacttt
    10741 attgttagat ggtttaatta ctggttttgg tattgctgct gttcattatg ctttagtccc
    10801 tatgctcgtt tttatcgcca tgcttaatgc aactgtatta ggctttggta gtttattttt
    10861 atgggttttt acaggatttt tttgtgtatt aggctcattg ttaggtggct ttgcatttgg
    10921 attagaaccc aatgtccatg aaagtattcc tatgtctgtg aatgccgttg cacttgttgg
    10981 attagctgtt tattttgggc ttaattcttt tcactctcgt gcccaatctc gtgatttaat
    11041 aactgctaaa caagagctac aagagcaaaa agaagagtca ttccaactat cacgtaagct
    11101 tatcaaatac ctccctcctc aagtttggga atcgatcttt aaaggcaaac gtgatgccaa
    11161 actacaaacc caacgcaaaa agctaactat tttcttttct gacatcaaag actttaccga
    11221 aacatctgac gaaatgccgc cagatttact caccgaaatg ctnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnaatacagg ctattgtcat gtgggtaact ttggttcaga
    11401 aactcggatg agttatacca ttattggtcg agatgccaac ttagccgctc ggttacaaag
    11461 tattgctgcg gttgatgaaa ttcttatttc tcacgaaacc tatttattaa ttcgtgacca
    11521 aatcttgtgt aaagaaaaag gggctgttac cttacgtggt attgccaaac cgactcaagt
    11581 gtgggaagtg gttgactttc acaaagatat taacagccat agtagctgga ttgaacacga
    11641 gttatcgggt tttaccatgc acatggattt aagcaaaatt cgtaattttg agaaagaagc
    11701 cgtgttaaaa gcacttgagc aagccgagaa aaaattacgc gacacaaaga ttttgtaatt
    11761 gtagggatag cccttgtggt tatcctaatt aacaaacgta cacatattca tatcatcgga
    11821 caaccacaag ggttgcccct acatcatcta taaatatatg aacaaaaatc tgattgcgac
    11881 attactctta ggagctgttt tgggagctag tggcaattat tatttgcaac aacgccctat
    11941 taaagtcact cttattaagc cgcaaatcag ccaattacaa agcactattt tagcaacagg
    12001 ccaagtcaaa aatagttctc acaccacttt acataatgaa aatgcaggtc taattaacca
    12061 agttctggaa gaaggtacgg tagtcagcaa aggacaaacg attgctttta ttcaagataa
    12121 agaaattgaa ccactgctgc tacaacaaca agccaatgtc aaaaatgccc aactcaaact
    12181 gaatcgttta caaaccattg aacgccaaca agcagagctt aaagttgagc aagcccgaat
    12241 tttgttaaag caagctaaac gccaattaag tgatacccaa gccttagcac aacaacaatt
    12301 aacctcgcaa aacaacttaa tcacggcgca agaaacctta agtttacgtg aaaaagaatt
    12361 agctaccgcc cttttgcaac aacaagcggt tcaggcacaa ggtttagacg aacaaactgc
    12421 gcttaaccaa ctgcaacaag cccaagcatt attagcccaa agtcaaattc ggcaacaacg
    12481 tcaaacgatt attagtcctt ttgatggcgt tattactgaa agaaaagtga gtgttggtca
    12541 atacgtcaaa caaggtgaag ccattgttgc tcttagccca aaacaagcgg ctgaaattat
    12601 tgctaacgtt gatgagcgtt ggttaccaca actccacctc aaacaacaag cttcagtgat
    12661 tgctgatgcc tttccacaac aagcttttac agctcaaatt agctatattt cccctaccat
    12721 tgataatagt cgtggaacgg ttgaaattcg tttacaaaac acacaatggc caagcttttt
    12781 acaagaaggc atgacggttt ctttagaatt attaagccaa gctgtgcaaa aaacagtgat
    12841 tattccaagc aaattgctcc aacaaaacga taatcaatat tgggtttggt tagcaaaaaa
    12901 tcagcaagca gaacgtcaaa atgtgcgtat tggtttgcgt catttagatc aagtacaaat
    12961 tattgagggc ttggatgaaa atagccaatt aattgatagc ccaactcccc tacaagctaa
    13021 acaagcaatt caggcgcact aacaaatggt ctggtttgaa tggcaactcg ccacacgctt
    13081 tcttaaagaa ggtaaaaccc aaactttgtt aattgccagt ggcattgcct tgggtgtcgc
    13141 ggtcattgtc tttattacta cgctagtgac tggcttacaa cggacgatta ttgatcgaac
    13201 tttaggcaca caagcacata ttgtgttaac cgagcctgat aaggagttac tggctagtgt
    13261 taagggtgaa caagtttttc ggcaaactca accccgccca caacgcatag aaccaattct
    13321 aaactggcaa ttgattgaac aaaaaatcaa aactgacccc caaattaaag ccgtttctcc
    13381 cgtgttatct ggtgcagcct ttgccttacg tggccaagct gacaaatcag ttgcgatatt
    13441 aggcattcaa cccgaagcct ataaccgtat cattccactt aaagactatc ttaaacaagg
    13501 ccaactacag cttaatagta atgaggcgat gattggtaca cgtttggcgg atgatttagg
    13561 gatacgtgtg ggtgataatt tgaatttatt aaccgccaat aatcgacaaa tttcggtgcg
    13621 aattacgggt atttttgaat ttggcattaa agatttagat cgtcgttggg tgtatttgtc
    13681 gttacgctcg gcacaaaatt tacttgattt acaaggtcaa attacaggca ttgatttaca
    13741 agtttatagc ttatttgatg ctgaaaaaat tgctcaacaa ttacaacaac aatacccgat
    13801 taaagccgaa agttggatga gcagcaacca acagttaatg acagccctac gctcacaaac
    13861 agcttcaacc acgctaattc gattttttgt gttaatttca gtggcctttg gtattgccag
    13921 tgtcttagca attgcggtgg tacaaaaaca aaaagaaatt ggtattttac gggcaatggg
    13981 tttaagccaa cgctcggtgt tggtggtatt tttaattcaa ggtggtttat taggctttat
    14041 tggctcaatt ttaggttctt tggctggcat gggcttggtt aaaatcttta ccttattcgt
    14101 taaaagtgca acaggcgaac ctttatttca attggctctg actcaagaag tgtttgtcac
    14161 caccatgctt gttgccacgt tgactggttt aatttctgcg gcaatgcctg cccttcgtgc
    14221 cgctaaactt gaccctgtac aggcgattcg ttatggctaa ctcggt
//
LOCUS       DAOQMB010000018        14140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1583_length_14140_cov_17.698687, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000018 DAOQMB010000000
VERSION     DAOQMB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 14140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14140
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1583_length_14140_cov_17.698687"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..464)
                     /gene="rplQ"
                     /locus_tag="PLY05_01500"
     CDS             complement(84..464)
                     /gene="rplQ"
                     /locus_tag="PLY05_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HNC02006.1"
                     /translation="MRHRNSGVKLGRNSSHRHAMFQNLAISLFEHELIHTTLPKAKEL
                     RRVAEPLITLAKVDSVANRRLAFARTRNKAIVAKLFNELGPRYKTRNGGYLRVLKAGF
                     RAGDAAPMAYVELVDRPAPQAAAE"
     gene            complement(528..1532)
                     /gene="rpoA"
                     /locus_tag="PLY05_01505"
     CDS             complement(528..1532)
                     /gene="rpoA"
                     /locus_tag="PLY05_01505"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNC02007.1"
                     /translation="MTRTNEFLTPRTIAVNAISTTHAKVVLEPLERGFGHTLGNALRR
                     ILLSSLPGAAVVEAEIEGVDHEYSTLEGLQEDIIELLLNLKGLAIKLFEQNEAVLTVD
                     VKGPGVVTAANISLPHNVEVVNLDHHIATLGNNGHLKMKLKVARGRGYEPVDARYAEG
                     ETRPIGRLQLDASYSPIKRVAYVVESARVEQRTDLDKLVIDLETNGTIEPEEAIRRAA
                     TILQQQIAVFVDLQKDHQPVAKAEPETVDPVLLRPVDDLELTVRSANCLKAENIYYIG
                     DLVQRTEVELLKTPNLGKKSLTEIKDVLASKGLSLGMRLDNWPPASLRADDRFAYRSR
                     "
     gene            complement(1572..2195)
                     /gene="rpsD"
                     /locus_tag="PLY05_01510"
     CDS             complement(1572..2195)
                     /gene="rpsD"
                     /locus_tag="PLY05_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNC02008.1"
                     /translation="MARYIGPKCKLSRREGTDLMLKSGVRALDSKCKIDTPPGVHGAS
                     RKGKLSDYGTQLREKQKVRRIYGVLERQFSNYYKEAARLKGATGENLLQLLESRLDNV
                     VYRMGFGATRSEARQLVSHRSITLNGRRVNIASIQVQPGDVVAVYEKSKNQLRIKSAI
                     ELAGQRGTHSWLDVDYSKMEGTFKALPERADLPAEINESLIVELYSK"
     gene            complement(2211..2600)
                     /gene="rpsK"
                     /locus_tag="PLY05_01515"
     CDS             complement(2211..2600)
                     /gene="rpsK"
                     /locus_tag="PLY05_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNC02009.1"
                     /translation="MAKDNQRNRKKVNRTVSDGMAHIHASFNNTIVTITDRQGNALAW
                     ATSGGQGFRGSRKSTPFAAQVAAEVAGKAAQEYGLKNLDVLVKGPGPGRESAVRALNA
                     VGYKINSITDVTPIPHNGCRPPKKRRV"
     gene            complement(2623..2979)
                     /gene="rpsM"
                     /locus_tag="PLY05_01520"
     CDS             complement(2623..2979)
                     /gene="rpsM"
                     /locus_tag="PLY05_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNC02010.1"
                     /translation="MARIAGVNIPDNKHAVISLTYIFGIGRTTAAKILATVGITPTTK
                     IKELSDSQLDALRAEVAKLPTEGDLRREISMNIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKAIKK"
     gene            complement(3076..3192)
                     /gene="rpmJ"
                     /locus_tag="PLY05_01525"
     CDS             complement(3076..3192)
                     /gene="rpmJ"
                     /locus_tag="PLY05_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNC02011.1"
                     /translation="MKVRASVKKICGSCKVVRRNGTIRVICSAEPRHKQRQG"
     gene            complement(3246..4547)
                     /gene="secY"
                     /locus_tag="PLY05_01530"
     CDS             complement(3246..4547)
                     /gene="secY"
                     /locus_tag="PLY05_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HNC02012.1"
                     /translation="MQSPGMRQGLSELWGRIIFLFVALVVFRFGAHVPVPGINPVKLA
                     QLFEQNKDTILSLFNMFSGGALERMSILALGIMPYISASIIMQLMTTVDPRLEALKKE
                     GEAGRRKINQYTRYGTLILAFVQGLGMCSGLVSQGITLSTGIAFFIPAVVSLVAGAMF
                     LMWLGEQITERGIGNGISMLIFAGIVAGIPSAIGMSLEATRQGEMSMLALLILFILAI
                     VVVGAVIFMERAQRRITVNYAQRQQGRRMYAAQQSHLPLKINMAGVIPAIFASSLLLF
                     PASIGQWFAQSPNPSTAQRVIQDITLVLSPGQPLYLLLFAILIIFFCYFYTALMFNPK
                     EVADNLKRSGAYIPGIRPGDQTAKYIDSVLTRLTLIGAIYITAVCLMPMLLQVAFNVP
                     FYLGGTSLLIVVVVVMDFMSQVQAHLMSHQYGSLMKKANLK"
     gene            complement(4589..5023)
                     /gene="rplO"
                     /locus_tag="PLY05_01535"
     CDS             complement(4589..5023)
                     /gene="rplO"
                     /locus_tag="PLY05_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNC02013.1"
                     /translation="MRLNELSPAEGSKFAGKRLGRGIGSGLGKTGGRGVKGQTSRSGG
                     GIRPGFEGGQMPIYRRLPKFGFTSLKSQVTAEVRLSELVYVEGEEVTLASLKAANVVR
                     KDMVRARIILSGEIGRAVTVKGVVVTKGARAAIEAAGGKVEE"
     gene            complement(5028..5210)
                     /gene="rpmD"
                     /locus_tag="PLY05_01540"
     CDS             complement(5028..5210)
                     /gene="rpmD"
                     /locus_tag="PLY05_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNC02014.1"
                     /translation="MTQATIKVTQIRSSAHKLKNHKLCLKGLGLRRIGHTVEVLDTPS
                     NRGMINKVYYLVKVGE"
     gene            complement(5215..5712)
                     /gene="rpsE"
                     /locus_tag="PLY05_01545"
     CDS             complement(5215..5712)
                     /gene="rpsE"
                     /locus_tag="PLY05_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNC02015.1"
                     /translation="MAKVEQNEGFVEKLVAVDRVAKVVKGGRIFSFTALTVVGDGKGK
                     VGFGRGKAREVPAAIQKALEAARRNMISVELNGTTLQHEIVAQHGASRIYMQPASEGT
                     GVIAGGAMRAVLEVAGVQNVLAKCYGSTNANNVVHATFKGLASMSSPEQVAAKRGKTV
                     EEILG"
     gene            complement(5714..6064)
                     /gene="rplR"
                     /locus_tag="PLY05_01550"
     CDS             complement(5714..6064)
                     /gene="rplR"
                     /locus_tag="PLY05_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNC02016.1"
                     /translation="MNEKKEARLRRAKSTRLKIRELGAVRLCVNRTPRHIYAQVISAC
                     GGKVLAQASTLDANLRDGATGNVEAAKKVGLLIAERAKAAGVTKVAFDRSGFQYHGRI
                     QALADAAREGGLEF"
     gene            complement(6080..6613)
                     /gene="rplF"
                     /locus_tag="PLY05_01555"
     CDS             complement(6080..6613)
                     /gene="rplF"
                     /locus_tag="PLY05_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNC02017.1"
                     /translation="MSRVAKAPVALPKGVEVTLNGQDLQVKGSKGVLSLSLHELVEAK
                     QEGETLVFTPRQNTQAAWMQTGTARALANNVVKGVSAGFERKLQLIGVGYKAAAKGKV
                     LNLALGFSHPIDYELPEGVTAETPTATEIILKSANKQLLGQVAANVRAFRAPEPYKGK
                     GVRYADEVVLRKEAKKK"
     gene            complement(6625..7008)
                     /gene="rpsH"
                     /locus_tag="PLY05_01560"
     CDS             complement(6625..7008)
                     /gene="rpsH"
                     /locus_tag="PLY05_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNC02018.1"
                     /translation="MSMQDTVADMLTRIRNAQLARKQTVAMPVSKLKTAIAKLLLDEG
                     YISSVEPEGLSLTLGLKYYEGKPVIEQIKRVSRPGLRIYKSKDELEPVKQGLGILIVS
                     TNKGIMTDRAARAAGVGGEVIALVS"
     gene            complement(7021..7326)
                     /gene="rpsN"
                     /locus_tag="PLY05_01565"
     CDS             complement(7021..7326)
                     /gene="rpsN"
                     /locus_tag="PLY05_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HNC02019.1"
                     /translation="MAKQSMINREAKREKAVAKYAELRQQLKATIKNVNATDEERWAA
                     MLKLQSLPRNSSPIRLRNRCGVTGRPHGYFRKFGLARTKLREAVMQGDVPGVVKASW"
     gene            complement(7336..7872)
                     /gene="rplE"
                     /locus_tag="PLY05_01570"
     CDS             complement(7336..7872)
                     /gene="rplE"
                     /locus_tag="PLY05_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNC02020.1"
                     /translation="MARLRGVYKNELALKLKEELGLENVMQVPRITKITLNMGVGGAA
                     QDKKMLDGAVADMQLISGQKPAITLARKSIAGFKIREGWPIGCKVTLRGDRMYEFLDR
                     LISITIPRIRDFRGFSPKAFDGRGNYSMGIKEQIVFPEIDFDKISQIRGLDITITTTA
                     ATDDQGRALLRAFGFPFK"
     gene            complement(7887..8204)
                     /gene="rplX"
                     /locus_tag="PLY05_01575"
     CDS             complement(7887..8204)
                     /gene="rplX"
                     /locus_tag="PLY05_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNC02021.1"
                     /translation="MAKIKKGDEIIVITGKDKGKRGTVVSVAEERVTVSGINLVKKHV
                     KPNPARGTQGGIVEKEAPLAISNVALINPETQKADRVGYKLLEDGTKVRVFKSNGAVI
                     NAK"
     gene            complement(8216..8584)
                     /gene="rplN"
                     /locus_tag="PLY05_01580"
     CDS             complement(8216..8584)
                     /gene="rplN"
                     /locus_tag="PLY05_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNC02022.1"
                     /translation="MIQTETMLEVADNSGARRVQCIKVLGGSHRRYATVGDIIKVTVK
                     EAIPRGRVKKGDVMNAVVVRTKFGIRRPDGSLIRFDDNAAVLLNNQKAPIATRIFGPV
                     TRELRTEQFMKIISLAPEVL"
     gene            complement(8710..8970)
                     /gene="rpsQ"
                     /locus_tag="PLY05_01585"
     CDS             complement(8710..8970)
                     /gene="rpsQ"
                     /locus_tag="PLY05_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNC02023.1"
                     /translation="MTEQTNTQRTLTGKVVSNKMDKSITVLIERLVKHPKYGKYIRRS
                     TKLHAHDENNVCQEGDIVTIAECRPLSKTKAWRLVEVVESVK"
     gene            complement(8972..9163)
                     /gene="rpmC"
                     /locus_tag="PLY05_01590"
     CDS             complement(8972..9163)
                     /gene="rpmC"
                     /locus_tag="PLY05_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNC02024.1"
                     /translation="MKATELRQKTVEELKAELDSLQLQQFKLRMAKATGQLAKTHQVG
                     LVRKDIARIKTVLTEKQGG"
     gene            complement(9163..9576)
                     /gene="rplP"
                     /locus_tag="PLY05_01595"
     CDS             complement(9163..9576)
                     /gene="rplP"
                     /locus_tag="PLY05_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNC02025.1"
                     /translation="MLQPKRTKFRKVHKGRNTGLAHRGSTVAFGSIALKSLERGRITA
                     RQIEAARRTITRRVKRGGKIWIRVFPDKPITEKPLEVRMGNGKGNVEYWVAEIQPGKI
                     LYEIEGVSEELARQAFTLAAAKLPVKTTIVTRTVM"
     gene            complement(9516..10350)
                     /gene="rpsC"
                     /locus_tag="PLY05_01600"
                     /pseudo
     CDS             complement(9516..10350)
                     /gene="rpsC"
                     /locus_tag="PLY05_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(10364..10693)
                     /gene="rplV"
                     /locus_tag="PLY05_01605"
     CDS             complement(10364..10693)
                     /gene="rplV"
                     /locus_tag="PLY05_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003656983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HNC02026.1"
                     /translation="MEAVAKLRGAPISAQKARLVADLVRGKPVEQALNTLEFSTKKAA
                     HFVKKVLESAIANAEHNYGADVDELKVATIFVDEGITLKRIMPRAKGRADRISKRTCH
                     ITVKVAE"
     gene            complement(10704..10979)
                     /gene="rpsS"
                     /locus_tag="PLY05_01610"
     CDS             complement(10704..10979)
                     /gene="rpsS"
                     /locus_tag="PLY05_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HNC02027.1"
                     /translation="MPRSLKKGPFVDTHLFAKVEAALATNSRKPIKTWSRRSMILPEM
                     VGLTIAVHNGRQHVPVLVSEHMVGHKLGEFAPTRTYRGHGVDKKAKR"
     gene            complement(11001..11825)
                     /gene="rplB"
                     /locus_tag="PLY05_01615"
     CDS             complement(11001..11825)
                     /gene="rplB"
                     /locus_tag="PLY05_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNC02028.1"
                     /translation="MPIVKCKPTSPGRRFVEKVVNPDLYKGRPYAPLVESKKRTGGRN
                     NNGHITTRHIGGGHKQLYRIVDFKRNKDGVPAVVERLEYDPNRSANIALLKYADGERR
                     YIIAPKGLKVGDAVQSGDDAPIKVGNALPLRNMPLGATLHCMELKAGKGAQLARSAGT
                     SVQLLGRDGAYAIVRLRSGEMRKIHVDCRATFGEVSNSEHNLRSLGKAGASRWRGVRP
                     TVRGTAMNPVDHPHGGGEGRTFGKHPTTPWGVQTKGYKTRSNKRTTNMIVRDRRVK"
     gene            complement(11836..12135)
                     /gene="rplW"
                     /locus_tag="PLY05_01620"
     CDS             complement(11836..12135)
                     /gene="rplW"
                     /locus_tag="PLY05_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNC02029.1"
                     /translation="MNNERLYQVILGPHISEKANIVAETQNQVVFKVRTDANKLEIKK
                     AVEKLLNVKVEAVNTVLVKGKTKRFGRTSGRRSDFKKAYITLPVGTEVQLADVAE"
     gene            complement(12132..12734)
                     /gene="rplD"
                     /locus_tag="PLY05_01625"
     CDS             complement(12132..12734)
                     /gene="rplD"
                     /locus_tag="PLY05_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNC02030.1"
                     /translation="MNLKTVSGAAVELSEVAFGKEFNEALVHQVVTAYLAGGRQGTRA
                     QKTRAEVSGGGKKPFKQKGTGRARAGSIRSPLWRGGGKIFAAKPQDWSQKVNRKMYRG
                     AMQCILAELVRQERLILVEEISVSAPKTKELLAKLKGLGAENALIVTEAVDENLYLAA
                     RNLPHVAVIDTAAVDPVSLIGFDKVLMSVPAAKKLEVELG"
     gene            complement(12747..13385)
                     /gene="rplC"
                     /locus_tag="PLY05_01630"
     CDS             complement(12747..13385)
                     /gene="rplC"
                     /locus_tag="PLY05_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNC02031.1"
                     /translation="MAIGLVGRKCGMTRIFTEAGNSIPVTVIEIDPNRITQIRTLETD
                     GYQAVQVTTGERRASRVTKAMAGHFAKASVAAGRGVWEFRVSGEEAASYAVGGSIGLD
                     IFAVGQVVDVIGTSKGKGFQGGVKRWNFRTQDATHGNSVSHRVHGSTGQNQSPGRVFK
                     GKKMAGHLGDERVTVQGLEVISVDVERSVLLIKGAVPGATGYDVIVRPSVKA"
     gene            complement(13439..13750)
                     /gene="rpsJ"
                     /locus_tag="PLY05_01635"
     CDS             complement(13439..13750)
                     /gene="rpsJ"
                     /locus_tag="PLY05_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNC02032.1"
                     /translation="MANQRIRIRLKAFDHRLIDQSAQEIVETAKRTGAQVCGPIPMPT
                     RIERFNVLTSPHVNKDARDQYEIRTHKRLVDIVQPTDKTVDALMKLDLAAGVDVQISL
                     G"
BASE COUNT         4014 a         3469 c         2776 g         3881 t
ORIGIN      
        1 agagaagcat taagtgataa aaatgttatt ttattttgac aataaaaaag ccgacataaa
       61 gtcggctttt ttatgatttt tgcttattca gcagctgctt gtggagccgg acgatcgacc
      121 agttctacat aagccatcgg tgctgcatca ccagcacgga aaccagcttt aagcacacgt
      181 aaataaccac cattacgggt tttataacgt ggacctaact cgttaaataa cttagcaaca
      241 atagccttgt tacgggtacg agcaaaagct aaacgacggt tagcaacgga atctacttta
      301 gctaaagtaa tgagtggctc agcaacgcgg cgcaactctt tcgccttagg taatgtagta
      361 tgaattaact catgttcgaa caaagaaatc gccaagtttt gaaacatagc atgacgatga
      421 ctgctgttac gaccaagttt gacaccactg ttacgatgac gcatggctaa aacttcctac
      481 aaagtcaggg cttaaatatc gctatctaag ccctaaaggt taagcgatta gcggctacga
      541 taggcaaaac gatcgtcagc acgcaaacta gctggaggcc agttatctaa acgcatacct
      601 aacgacaagc ctttggaagc caacacatct ttaatttctg tcagtgactt cttacccaag
      661 tttggtgtct tgagtaattc aacttcagtg cgttgaacca aatcaccgat atagtaaata
      721 ttttcagctt tgagacagtt agccgaacgt acagttaact ctaaatcgtc aacaggacgt
      781 aacaatactg gatcaactgt ttcaggttct gctttagcaa ctggctgatg atctttttgt
      841 aaatcaacaa acacagcgat ttgctgttgc agaatcgtag ctgcacgacg aattgcttct
      901 tctggctcga ttgtaccgtt agtttctaaa tcaataacta acttgtcgag gtcagtacgc
      961 tgttcaacac gagcactttc tacaacataa gcaacacgtt taatcggact gtaacttgcg
     1021 tctaactgca agcggccaat cggacgagtc tccccttccg cgtaacgtgc atctacaggc
     1081 tcatagccac gaccacgcgc cactttcagc ttcatcttca agtgaccatt gttacccaat
     1141 gttgcgatat gatgatctaa gttcaccact tcaacattgt gcggcaaact aatatttgct
     1201 gcagtaacaa cgcctggacc ttttacatcc acagttaaca ctgcttcgtt ttgctcaaag
     1261 agtttgattg ctagaccttt taagtttaac aataattcaa taatatcttc ttgcaaacct
     1321 tcgagtgtac tgtactcgtg atccacacct tcaatttcag cttctactac cgctgcacct
     1381 ggcaacgaag aaagcagaat acgacgcaaa gcattgccaa gtgtatgacc aaaaccgcgt
     1441 tctaacggct ctaatacaac tttggcgtga gtcgtactga ttgcattaac agcaatagta
     1501 cgaggagtca aaaactcatt ggtgcgcgtc atggaatcac ctcaaaacca ctcaaaactg
     1561 gggtaattac cttacttaga gtacaactca acaatcaagc tttcgttgat ttcagctgga
     1621 agatcagcac gctctggcaa tgctttaaat gtaccttcca ttttactgta atcaacatct
     1681 aaccaagaat gtgtgccacg ttgacctgcc aattcaatcg cgctcttaat gcgtaattga
     1741 tttttagatt tttcgtacac agcaactaca tcacctggtt gaacctgaat cgaagcgatg
     1801 tttacacgac gaccatttaa ggtaatcgaa cgatggctaa ctaattgacg tgcttcagag
     1861 cgagttgcac caaaacccat acggtaaacc acgttgtcta aacggctttc taaaagttgc
     1921 aataagtttt caccagttgc accctttaag cgagctgctt ctttgtaata attgctgaat
     1981 tgacgctcaa gaacaccata aatacggcga actttttgct tttcacgcaa ctgagtacca
     2041 taatcagaga gcttaccttt acgagaagca ccatgtacgc caggtggtgt atcaatttta
     2101 catttagagt ctaacgcacg aacgccagac ttcagcatca aatccgtgcc ttcgcgacgg
     2161 gagagcttgc acttaggacc aatataacgt gccattcttc aagtctcctg ttacacgcga
     2221 cgttttttag gaggacgaca gccattatga ggaattggcg tcacgtctgt gatactgttg
     2281 attttataac ctacagcatt taatgcacga accgcggatt cacgaccagg ccccggacct
     2341 ttcacgagta cgtctaagtt ttttagaccg tattcttgag ctgctttgcc tgcaacttcg
     2401 gcagcaacct gcgcagcaaa gggggtagat ttacgcgaac cacggaagcc ctgaccaccg
     2461 ctagtggccc aggccagtgc attaccttga cgatcggtaa ttgtcacaat ggtgttatta
     2521 aaagaagcgt gaatatgcgc cataccatca gaaacggtgc gattgacctt tttccgattg
     2581 cgctgattgt cttttgccat cttttagctt ccgaggggct aattactttt taattgcctt
     2641 acgtggacct ttacgcgtac gagcgttagt tttcgtacgt tggccacgaa ctggcagacc
     2701 acgacgatga cgtaaaccgc ggtagcagcc taagtccatc aatcgtttga tgttcatcga
     2761 gatttcacga cgtaaatcac cttcagtagg taatttagcc acttcagcac gaagtgcatc
     2821 taattggcta tctgacaact ctttaatttt agtggttggt gtaataccga cagttgctaa
     2881 aattttagca gctgtagtac gaccaatacc aaaaatataa gttaacgaga taaccgcgtg
     2941 tttgttatcg ggaatattaa cgccggctat acgagccatt cacctgttct ccacacaaag
     3001 cggttgcgat aatccgctaa gccacgaaaa gtgggcgatt ttatcgcctt agcttgcgga
     3061 aatcaacagc gaagtttaac cttgacgctg tttatgacgg ggttccgcgc tacaaataac
     3121 gcggatggtg ccattgcggc gtacaacttt gcagctaccg caaatctttt ttactgaagc
     3181 acgaactttc ataattcacc tacttagcta aacaaaaatc ttaactaatt gttctaatag
     3241 aacttttact taagattagc ttttttcatc aatgatccat attgatgaga cattaaatgt
     3301 gcttgtactt gtgacataaa atccatcaca actacaacaa caattaataa cgatgtacca
     3361 cctaaataaa atggcacatt aaaagcaacc tgtaacaaca tgggcattaa acaaactgcc
     3421 gtgatatata ttgcgccaat taatgttaag cgagttaata cactatcaat atattttgca
     3481 gtttggtcac ctggacgaat accaggaata taagcacccg aacgttttaa attatctgct
     3541 acttctttag gattaaacat taaagctgta taaaaatagc agaagaaaat aattaaaatt
     3601 gcaaacaata ataaatataa aggctgtccc ggtgataaaa ccaaagtgat atcttggatc
     3661 acgcgttgcg cagttgatgg attaggcgac tgagcaaacc attgaccaat actggctgga
     3721 aacaacaata aagagcttgc aaaaattgca ggaataacac ctgccatatt aatttttaac
     3781 ggtaaatggc tttgttgagc agcatacata cgacggcctt gctgacgctg agcataattt
     3841 accgtaatac gacgttgggc gcgttccata aaaataacag caccaaccac aacaattgcc
     3901 aaaataaaca aaatcaataa agctaacatg ctcatttcgc cttggcgagt tgcttctaag
     3961 gacataccaa tagctgaagg tatgccagca acaataccag cgaaaatcaa catagaaata
     4021 ccattaccaa taccacgttc ggtaatttgt tcacccaacc acattaagaa cattgctcca
     4081 gccaccaagc tgaccacagc aggaataaaa aatgcgatac ctgtagacaa agtaatacct
     4141 tgactaacta ggccagaaca cataccaaga ccttggacaa aggctaaaat taatgtaccg
     4201 tatctagtat attgattaat tttacgacgg cctgcctccc cttctttctt taatgcttct
     4261 aaccgtggat caaccgtggt cattagctgc ataataatag aagcggaaat ataaggcata
     4321 atccctagag ctaaaattga catacgctct aaagcaccgc ccgaaaacat attaaataaa
     4381 cttaatattg tatctttatt ttgttcaaat agctgtgcca gcttcacagg gttaatccct
     4441 ggaactggca catgtgctcc gaatcggaat accactaacg cgacgaacaa aaagataata
     4501 cgtccccaaa gctcacttaa gccttgacgc ataccaggcg actgcatagc cgcacgtgct
     4561 gcctcattac gggtattaac cattcagatt attcctcaac cttaccgcca gcggcttcga
     4621 ttgctgcacg agcaccttta gtgacaacga cacctttcac tgtaacagcg cggccaattt
     4681 caccagatag gataatacga gcacgaacca tgtctttacg tacgacatta gctgctttta
     4741 aggatgctaa agtaacttct tcaccttcaa cataaaccaa ttcagataaa cgcacttcag
     4801 ctgttacctg agattttaac gaagtgaagc caaactttgg caaacgacga taaattggca
     4861 tttgaccgcc ttcgaaacca gggcgaatac cgccgccaga acgagaggtt tggcctttaa
     4921 caccacgacc accagtctta cctaagccag agccaatacc acgacctaag cgtttacctg
     4981 caaacttaga gccttcagca ggacttaatt catttaagcg catggattta ttctcccact
     5041 ttcacaaggt aataaacctt gttaatcatg ccacggttag aaggtgtatc taacacttct
     5101 acagtatgac caatacgacg taagcctaaa cctttcaagc agagcttgtg attttttaac
     5161 ttgtgcgcac tggagcgaat ttgcgtaact ttaatagttg cttgggtcat gttcttaccc
     5221 cagaatctct tctacagttt taccgcgttt cgctgcaact tgctctggcg aagacataga
     5281 agccaagcct ttgaaagtag catgtaccac gttgtttgca ttagtagagc cgtagcactt
     5341 tgctaatacg ttttgtacac cagcaacttc taatactgca cgcattgcac caccagcgat
     5401 gacaccagta ccttcagagg ctggctgcat gtaaatacga gaagcaccgt gttgagcaac
     5461 aatttcgtgc tgcaaggttg taccattcaa ttcaacgcta atcatattac gacgagcagc
     5521 ttctaaagct ttttgaatag cggctggtac ttcgcgggct ttaccacgac caaaaccaac
     5581 tttaccctta ccatcgccaa caactgttaa cgcggtgaac gagaaaatac gaccaccttt
     5641 aacaacttta gcaacacgat ccacggcaac gagcttctca acaaagcctt cgttttgttc
     5701 aactttagcc atcttagaac tccaaaccgc cttcgcgggc agcatcagct aaagcttgaa
     5761 tgcggccatg atattgaaag ccagaacggt caaatgctac tttagtaaca ccagcagctt
     5821 tagcacgttc agcaattaac aaacctactt ttttagcggc ttcgacgttg cctgttgcac
     5881 catcacgtaa attagcatct aaagtagagg cttgagctaa gacttttcca ccacaagcag
     5941 aaatgacttg agcataaata tgacgcggcg tgcggttgac acacagacgt acagcaccca
     6001 gctcgcgaat tttcaaacga gtgcttttgg cgcgccgtaa gcgagcttct ttcttttcgt
     6061 tcatgacgaa cctcacgcgt tatttcttct tagcttcctt acggagaaca acttcatccg
     6121 cataacgaac acctttacct ttatatggct ctggcgcacg gaaagcacga acattagcag
     6181 caacctgacc taacaattgc ttgttagcag atttcaaaat gatttctgtt gcggttggcg
     6241 tctcagctgt tacaccttct ggtaactcat aatcgattgg atgagagaag cctaaagcta
     6301 agttcaacac tttaccttta gcagctgctt tataacccac accgatcaac tgtaatttac
     6361 gctcaaaacc agcacttaca cctttaacaa cattgtttgc taaggcacgg gcagtacctg
     6421 tttgcatcca cgctgcttgt gtgttttgac gaggagtgaa cactaaagtt tcaccctctt
     6481 gtttagcttc gaccaattcg tgaagagaca gtgacaatac acctttgctg cccttcactt
     6541 gtaggtcttg gccattaaga gtcacttcta ccccttttgg gagagcaact ggagctttag
     6601 ccacgcgaga catgcttgca cctatcaaga aacgagagca atcacttcac cacccacacc
     6661 agctgcacga gcggcgcgat cggtcatgat gcctttgtta gtagaaacaa tgagaatacc
     6721 taaaccttgt ttaacaggtt ctaactcatc tttgctcttg taaatgcgta aacctggacg
     6781 gcttacacgt ttaatttgtt caataacagg tttgccttcg tagtatttta aacctaaggt
     6841 taatgataaa ccttcgggtt ctacagaaga aatataacct tcatcgagta ataatttagc
     6901 gatcgctgtt ttcagtttag aaactggcat agcgacggtt tgtttacgag ctaattgcgc
     6961 gttgcgaatg cgtgtaagca tatcggcaac ggtatcttgc atactcatgc gtctttcccc
     7021 ttaccagcta gccttaacga cgcctggcac atcaccctgc atcacagctt cacgtaattt
     7081 agtacgtgct aaaccgaatt tacggaagta accgtgagga cgaccagtca caccacaacg
     7141 attacgtaaa cgaatcggtg aagaattgcg tggtaaagat tgcaatttca acattgcagc
     7201 ccaacgctct tcatcagtcg cattcacatt cttgatcgtt gcttttaact gctgacgtaa
     7261 ttcagcatat ttagcaacag ctttttcgcg tttggcttcg cggttaatca tgctttgttt
     7321 agccatggtg cgttctcact taaaaggaaa gccgaaagca cgcagtaacg cacgaccttg
     7381 atcgtcggta gccgcagtgg tggtaatggt aatatctaaa ccacgaattt gactgatttt
     7441 atcaaagtca atttcaggga atacgatttg ttctttgata cccatgctgt aattaccacg
     7501 gccatcaaaa gcttttggac taaaaccacg gaagtcacga atacgcggaa tcgtaatcga
     7561 aattaaacga tccaaaaatt cgtacatacg gtcgccacgt aaagtcactt tacaaccaat
     7621 tggccaacct tcacgaattt tgaagccagc aattgattta cgtgcgagag taatagcggg
     7681 tttctggcca gaaatcagct gcatatcggc cacggcacca tcaagcatct ttttatcttg
     7741 cgcggcaccg cccactccca tattcagagt aattttggtg atgcgaggga cttgcatcac
     7801 attctcaaga cctaactctt cttttaattt aagagctagt tcgtttttgt acacgcctct
     7861 tagtctggcc atgtcaaacc aacctattac ttggcgttga ttacagcacc attagatttg
     7921 aagacacgaa ctttagtgcc atcttctaat agtttgtaac ctacgcgatc tgccttttgc
     7981 gtctctggat taattaaagc aacattagaa atcgccaatg gagcttcttt ctctacgata
     8041 ccaccttgag taccacgagc cggattcggc ttaacgtgtt ttttaaccaa gttgatacca
     8101 cttacagtga cacgttcttc agcaacagac actactgtgc cacgcttgcc tttatcttta
     8161 ccagtgatga cgataatttc gtcacctttt ttaatctttg ccatgagtcc cacccttaca
     8221 gcacttctgg tgccagcgag atgattttca tgaattgctc agtacgcaat tcgcgagtta
     8281 ccggcccgaa aatacgggta gcgatggggg ctttttggtt attcagcaac accgcagcat
     8341 tgtcatcaaa acggattaat gaaccgtctg gacggcgaat accaaacttg gtacgcacaa
     8401 ccacagcatt catcacatcg ccttttttaa ccctgccacg aggaattgct tccttgaccg
     8461 tgaccttaat aatatcgccg acggtagcat aacgacgatg tgagccgccc agtactttga
     8521 tgcactgtac gcgacgcgcg ccactattgt ctgctacctc gagcatcgtt tcggtctgaa
     8581 tcattgctct actccaaacc tagataaccc catgcgaagg ggcggtaatc ctacccaata
     8641 tcgcacaggg atacaagtaa aaatcagctt atttagagat ttggcagaaa aaatctgcca
     8701 aatttcggat tatttcaccg attcaaccac ttcaaccaat ctccaagctt tagttttgga
     8761 gagtggacgg cattctgcga tggtgacaat atcgccttct tgacacacat tattttcatc
     8821 atgtgcgtgc aatttggtgg agcgacgaat gtacttaccg tattttggat gtttgacaag
     8881 acgttcaatc aacactgtaa tggatttatc cattttgttg ctgactactt taccggtcaa
     8941 tgtccgttgg gtattggtct gctctgtcat ctcagccacc ttgtttttct gtcaagactg
     9001 ttttgatacg ggcgatatct ttacgaacca accctacctg atgcgtcttg gcgagttggc
     9061 cagtcgcttt cgccatacgc agcttaaatt gctgcaactg gaggctatca agttcagcct
     9121 tgagttcctc gacagttttt tgacgtaatt ctgttgcttt cattacatca ccgtccgcgt
     9181 cacgatggtt gttttgacag gtaatttagc agctgccaag gtaaaagctt gacgcgctaa
     9241 ttcttcagaa acaccttcga tttcataaag aattttacct ggttgaattt cggcaaccca
     9301 gtattcaaca ttacccttac cgttacccat acgaacttct aatggctttt cggtaattgg
     9361 tttgtctggg aatacacgaa tccaaatttt accgccacgc ttaacacgac gagtaatcgt
     9421 acgacgggca gcttcaattt gacgtgcagt aatacgacca cgctcaagtg attttaacgc
     9481 gatgctacca aaagcaacgg tgctaccacg atgagctaag ccagtattac ggcctttatg
     9541 aaccttacgg aacttggtac gtttaggctg taacatagtt tattacccct ttgcttccgt
     9601 attgcggccg ccacgacggt cttcacgacc accacgatct tcacgatcat cacgaccacc
     9661 acgttgcttt ttagcgggct tgctttcttc tactggtgct ggttgcatag cttgagccat
     9721 accacctaaa atctcacctt tgaagatcca aactttaacg ccaatacaac cataagtggt
     9781 ttcagcacgc ataatcgagt aatcgatatc agcacgtaat gtatgcaaag gtacacgacc
     9841 ttcacgatac cattcagaac gcgcgatctc tgcaccacct aaacgaccag atacttcaac
     9901 tttgatgcct ttagcaccag cacgcatggt gttttgtaca gcgcgtttca tggcgcgacg
     9961 gaacattaca cgcttttcta attgtgctgc aatgctttca gcaactaaac gtgcatctaa
    10021 gtcaggcttg cgaatttcgt caatgttgat ttgaactgga gtcttcatca acttagacac
    10081 ttcgcgacct aaacgctcaa catcttcacc ttttttacca atgatgatgc caggacgtgc
    10141 cgtactaatg gtaatacggg ctgtctcaga tggacgctca attaaaatac gacttaccgc
    10201 ggcgctcttg agcttgctat acaagtactc acgaacagct aaatcagtaa gcaaaaaatc
    10261 cgcatagttt tttggactag cataccagtt ggcattgtgg cgtttaataa caccaagacg
    10321 aatgcctatt ggatgaactt tctgacccat ttcccgaccc cacttattcc gcgactttga
    10381 cggtgatatg gcaggtacgc ttgctgatac gatcagcacg gcctttagca cgtggcatga
    10441 tacgtttcaa ggtaatacct tcatcaacaa agatcgtggc gactttgagc tcatccacgt
    10501 ctgccccata attatgctca gcgttggcaa ttgccgactc gagcactttt ttaacaaagt
    10561 gggcagcttt tttggtgctg aattctaatg tattcagcgc ttgctccacc ggcttaccac
    10621 gcaccagatc agccactaaa cgtgctttct gggcagatat tggcgcgccg cgaagtttag
    10681 caacagcttc cattactcac cccttagcgt ttggctttct tgtcaacacc atgaccgcga
    10741 taagtgcggg ttggtgcgaa ttcgccgagt ttatggccga ccatatgctc actaactaaa
    10801 actggcacat gctgacgacc gttatgcaca gcgatggtga gacctaccat ttctggcaga
    10861 atcatcgacc gacgcgacca ggttttaata ggctttctgg agttggtagc caatgcggct
    10921 tcaactttcg caaaaagatg cgtatctacg aacgggcctt tcttaagtga acgaggcaca
    10981 gttagtctcc tcttttccta ttacttgacg cgacgatcac gtacaatcat attagttgta
    11041 cgcttgttac tacgagtttt atagcccttg gtctgcacgc cccacggagt ggttggatgc
    11101 ttaccaaaag tacgtccctc accaccaccg tgtggatggt caactgggtt cattgccgta
    11161 ccacgaacgg tcggacgaac accacgccaa cggctagcac cagctttacc caacgaacgc
    11221 aaattgtgtt cggagttaga aacttcgcca aacgtagcgc gacaatccac atggatttta
    11281 cgcatttcac cagagcgcaa acgcacaatg gcataggcac catcacgacc caataattgg
    11341 acagatgtac cagcagaacg agccaattga gcacctttac ctgcttttaa ctccatacag
    11401 tgcaaagtag caccgagtgg catattgcgt aatggtaaag cgttacccac tttgattggc
    11461 gcatcatcac cagattgcac tgcatcaccg acttttaagc cttttggtgc aatgatatag
    11521 cgacgttcac catcggcata tttcaataat gcgatatttg cagaacggtt tggatcgtac
    11581 tctaaacgct caacaacagc tggaacacca tctttgttac gtttaaagtc aacgatacga
    11641 tataactgct tatgaccacc gccaatatga cgagtagtaa tatgaccgtt attattacga
    11701 ccaccagttc tctttttgct ttcaactaaa ggtgcgtatg gacgaccttt atataagtct
    11761 ggattaacga ctttctcaac aaaacgacga cctggtgacg ttggtttaca ttttacgata
    11821 ggcataacgc gctccttact cagctacgtc agctaactga acttctgtgc ctacaggtaa
    11881 tgtaatgtaa gcttttttga agtcagagcg acgacccgag gtacgaccaa aacgctttgt
    11941 cttaccttta accaacactg tattaactgc ttcaacttta acattaagca atttttctac
    12001 agcttttttg atctccagtt tgttagcatc tgtgcgcact ttgaaaacca cttgattttg
    12061 ggtttcagcg acaatatttg ctttttcgga gatgtgcggg ccaaggatta cttggtataa
    12121 acgttcgttg ttcatcccaa ttccacctct aattttttag cggctggcac agacattaat
    12181 accttgtcaa aaccaatcaa actaactgga tcaacagctg cagtatcaat aacagccaca
    12241 tgtggcaagt tacgagcagc gaggtataag ttctcgtcaa cagcttcagt cacgatcaaa
    12301 gcgttttctg cgcctaaacc tttgagtttt gcaagcaact ctttagtttt tggtgcgcta
    12361 acactgattt cttcaaccaa aattaaacgt tcttgacgaa ccaactcggc caaaatacat
    12421 tgcatcgcgc cgcgatacat tttgcggttc actttttgag accagtcttg tggttttgca
    12481 gcaaaaatct tgccgccacc acgccacaat ggactacgaa tagaaccagc acgtgcgcgg
    12541 ccagtaccct tttgtttaaa aggcttctta ccaccaccac ttacttcagc acgtgttttt
    12601 tgagcacgag tgccttgacg accaccagcc aaataagcag tgacgacttg gtgcactaat
    12661 gcttcattaa attctttgcc gaaggcaact tcggacagct caacagccgc accggaaaca
    12721 gtcttcagat tcacgcttag ctccccttag gccttaaccg atggacgtac aatcacatcg
    12781 tagcctgttg cgcccggaac tgcaccctta attaaaagga cggaacgttc gacatcaacg
    12841 gaaatcactt ccaaaccttg tacggtaacg cgttcgtcac ccaagtgacc tgccatcttt
    12901 ttgcctttga acacgcgacc cggagattgg ttttgacccg tagaaccatg tacacgatga
    12961 gacacagagt taccgtgtgt tgcatcttgc gtacggaagt tccagcgttt tacaccgcct
    13021 tggaaacctt tacctttgct agtaccaatt acgtcaacta cttgacctac tgcaaaaata
    13081 tctaaaccaa tgctgccacc aactgcataa cttgcagctt cttcaccact tacacggaat
    13141 tcccatacac cacgaccagc agcaaccgaa gcctttgcaa aatggccagc cattgctttg
    13201 gtcacacggg aagcacgacg ttcaccagtg gttacttgta cagcttgata gccgtcggtt
    13261 tctaaggttc taatctgagt gatgcggttg ggatcgattt caatcaccgt aacaggtata
    13321 gaattacccg cttcagtgaa gatgcgcgtc atgccgcact tacgaccgac taaaccaata
    13381 gccatttcct ctaacctctt cttaacgctg cctcatgaca gcgcgatagg aaaaacgatt
    13441 aacccaaact gatctgcaca tcaacaccag cagcaagatc aagtttcatc aacgcatcaa
    13501 cagttttatc tgtaggttgc acgatatcca ctaatctttt gtgggtgcgg atctcatact
    13561 gatcgcgggc atctttattc acatgcggcg aagtcagcac attaaagcgc tcgatgcgag
    13621 tgggcatcgg aatcggacca caaacctgtg cgccagtgcg cttggcggtc tcaacgatct
    13681 cctgagcgga ttgatcaatc aaacgatgat cgaatgcttt caggcgaatg cggattctct
    13741 ggttagccat accagcaact ccaagccaaa aaataaaaaa gtcccttcgc ctgtttatat
    13801 agaacagcga aaagccataa atacgatgcc aagaacgatg ccttagctgt aaagtgtgtt
    13861 cttatttaac attaggaaca cattcttaac ccgactcttt agcgggtggc gaatgatagc
    13921 ccagtattca gggctttgca atctatttcc ctaaaaaaag ccaattattt tagagggatt
    13981 aaaaaaggag gccgaagcct ccttttgatt gccgtgttta ttatttattt gctcataatc
    14041 atgcacaaat aaacataatt tgcgtcaata ttttcaagtc gttttaatta cttgatgatt
    14101 ttagcaacaa caccagcacc gactgtacgg ccgccttcac
//
LOCUS       DAOQMB010000019        13559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1683_length_13559_cov_16.879739, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000019 DAOQMB010000000
VERSION     DAOQMB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1683_length_13559_cov_16.879739"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1830
                     /locus_tag="PLY05_01640"
     CDS             <1..1830
                     /locus_tag="PLY05_01640"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011280032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HNC02033.1"
                     /translation="HIPNKRDGGFCIGSKIAPIFFNTMEDAGALPVELDVSQMNMGDE
                     IVLNIDHANAIVTATKDGVEIAKAPLKTPVLLDEVRAGGRINLIIGRSLTTKAREALG
                     LAPSALFRQPQQPADTGKGYTQAQKMVGKACGVTGIRPGTYCEPKMTTVGSQDTTGPM
                     TRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKMHHTLPDFIMNRGGVALRPGDGI
                     IHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVKF
                     KGKMQAGITLRDLVHAIPYYAIQAGDLTVEKKGKKNIFNGRILEIDLSEMEGDLTVEQ
                     AFELSDASAERSAAGCSITLSEKSVAEYLTSNITMLKWMISEGYGDARTMARRIEAME
                     KWLANPVLLRADADAEYAKVYEIDLNDIKEPILCCPNDPDDAKLLSTVAGDKIDEVFI
                     GSCMTNIGHFRAAGKLLEKVEGGSLSTRLWLAPPTKMDAHQLMEEGYYNIYGRAGART
                     EMPGCSLCMGNQARVAPNTTCVSTSTRNFPNRLGQGSNVYLASAELASVAAVMGKLPT
                     VAEYMEYANKIDSMAGDIYRYLNFDRMSEYTDKANKIDVAQLT"
     gene            1906..2544
                     /locus_tag="PLY05_01645"
     CDS             1906..2544
                     /locus_tag="PLY05_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase domain-containing protein"
                     /protein_id="HNC02034.1"
                     /translation="MNLPLKIVVISDTHGLHDLLTIPDGDILIHCGDMTEYGLIEEIE
                     EFDEWLGTLPHKHKIVIAGNHELCLEQESGWALFHHATYLEHEAVTIEGVNFFGSPWQ
                     PYFHGMAFNLKRGEALAKKWQDIPDNTDILITHSPPYKYLDKVRSQWVGCADLLQRVQ
                     QVKPVYHVFGHIHESYGQESNRHTTFINACMVVELDAPLNKPMVIDWPKQLL"
     gene            complement(2536..3084)
                     /locus_tag="PLY05_01650"
     CDS             complement(2536..3084)
                     /locus_tag="PLY05_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC02035.1"
                     /translation="MAKNRRELDSEHKRQEILFAAKRLLLAEGYDATSMSRLAKEAGV
                     APNTLYWYFKDKDELLIAILDELVAEALAEYLSVQAQSLDKQLLWLLDKFDAVPHVIT
                     TVHNRMNFAESIKTWHQRFHFMLESLVVNQLMLHGLKQEECVAAAKIATFVVEGLLSH
                     HAGDASERATIAKFLISKITLQ"
     gene            complement(3172..3759)
                     /locus_tag="PLY05_01655"
     CDS             complement(3172..3759)
                     /locus_tag="PLY05_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNC02036.1"
                     /translation="MANPQQTNYMTEQEYLQQEQQSDLKHEYIDGQVYAMAGAKASHN
                     RIVGNFYRKVGNYLENKPCQPYMSDMQVKIASKYFYPDVLVDCSNLSDDSTFTETPIL
                     IVEVLSKSTRRADETTKRMAYMQIPTLLEYVLIEQDFVQIEVMSKNHHWQSFKYYLGD
                     DIYFESIGLCLKVEDIYERVQNSDMLEWLKNKTSQ"
     gene            complement(3778..4305)
                     /locus_tag="PLY05_01660"
     CDS             complement(3778..4305)
                     /locus_tag="PLY05_01660"
                     /inference="COORDINATES: protein motif:HMM:NF021360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HNC02037.1"
                     /translation="MSNLKAQLLKSGLADAKRAKKIHQQQLEAGKAAKDEAKNQVQKA
                     MAEKVERDRLLNLQLKEEQEKKAILAQIKQLIETNKIDRAGGEVAYQFTDDKKIKKLY
                     VTDLQFNQLIKGIIAIVRFANGYELVPTRVAEKIATRDANLVLVLNIKPEATATDTAE
                     EDPYADYQIPDDLMW"
     gene            complement(4381..5271)
                     /gene="rpoS"
                     /locus_tag="PLY05_01665"
     CDS             complement(4381..5271)
                     /gene="rpoS"
                     /locus_tag="PLY05_01665"
                     /inference="COORDINATES: protein motif:HMM:NF004207.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HNC02038.1"
                     /translation="MATSKAQTKKTTSVSHPTDATQLYLHDIGVSPLLSAEEEVHFAR
                     LAKAGEESARRRMIESNLRLVVKIARRYANRGLSFLDLIEEGNLGLIHAVEKFDPEKG
                     FRFSTYATWWIRQTIERALMSQTRTVRLPVHIVKELNIYLRAARELSQKLDHEPTAEE
                     IANLLDKPIATVEQMLGLTEKITSIDSSFSEDGDKSLLDTLSDPQTIDPADQIQTNEL
                     NHSLEHWLTLLPERQREVLMRRFGLAGFEASTLEDVGEQIGLTRERVRQIQVDALKRL
                     REILMREGLSADAIFNQTLH"
     gene            complement(5349..6224)
                     /locus_tag="PLY05_01670"
     CDS             complement(5349..6224)
                     /locus_tag="PLY05_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC02039.1"
                     /translation="MRVAQKSKWIFTTLIVALLAACSTAPKKLPTDAEKAKLTEKQPL
                     PPLSYPYYRVKAGDTLYSIAMRYDKDYKELGKINNLDEKFTIYVDQKLVLEDENAKPE
                     EKTSSISEPVKIVAKPLPPTNKPVVVKPKTDTTKPVNTNNNDLKPVVKNTPSVGTNVQ
                     ALAKSGSLTWGWPVNNGNNQLIKRFSTEGVGSKGIAIAGKRGDAINAAANGVVVYAGE
                     GLVGYGKLIIVRHSDEYITAYAHNDRLIATEGQKVQLGQKIAELGSTGADREKLHFEI
                     RYKGKPVDPLSILPN"
     gene            complement(6244..7020)
                     /gene="surE"
                     /locus_tag="PLY05_01675"
     CDS             complement(6244..7020)
                     /gene="surE"
                     /locus_tag="PLY05_01675"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNC02040.1"
                     /translation="MHILLSNDDGVFAPGLAALAKALSKIAKVTVMAPEAERSGYSSA
                     LTLDRPLRATQLDNGFWSVNGTPADCVYLALNGFFADDEPDFVVTGINSGANLGDDVI
                     YSGTVGAALEGRFLGKPALAVSLAGPKVKQHTSVDAFAVAAELVVDLVSHWHDLALPE
                     HNIINMNVPDIPANQIKGFRLTQLGKRQRGLSIQKSHDPRQRPVYWIGLMGDPVPTEH
                     ETDFQTVANGYVSLTPLQTDLTSHDALPALHAWLATKNTQ"
     gene            complement(7022..7519)
                     /gene="ispF"
                     /locus_tag="PLY05_01680"
     CDS             complement(7022..7519)
                     /gene="ispF"
                     /locus_tag="PLY05_01680"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNC02041.1"
                     /translation="MKYPRIGHGIDVHRFGDGDSVTLGGVKIAHHQGLIAHSDGDVAL
                     HALCDALLGALALGDIGKHFPDTDMAYKNADSRVLLRRVFALIEAKGYQLGNCDLTIL
                     AEKPKLAPHIALMQQRIAEDLATAVENVSIKATTTEKLGFVGRQEGIEAHAVVCLIPK
                     EDWQD"
     gene            complement(7516..8214)
                     /gene="ispD"
                     /locus_tag="PLY05_01685"
     CDS             complement(7516..8214)
                     /gene="ispD"
                     /locus_tag="PLY05_01685"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HNC02042.1"
                     /translation="MNYWLIVPAAGKGKRFGGQLPKQYLRLGQKTVMQSTLDRLSTLA
                     AIKQIIVPIHPEDDIAKKLTYQYPQKIRFVTGGAERADSVLAGLEAIKNEAQPDDWVL
                     VHDVARPCVRLTDIQNMFTQLLDDEVGGILAAPVRDTIKQASDDGQNHIIATVPRQQL
                     WQAFTPQMFRFDIIYQALSHALAQQNMVTDEASAVELLGFKPRLINGALDNLKITYPE
                     DLLLAEFLIRQQTT"
     gene            complement(8211..8549)
                     /gene="ftsB"
                     /locus_tag="PLY05_01690"
     CDS             complement(8211..8549)
                     /gene="ftsB"
                     /locus_tag="PLY05_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017196607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HNC02043.1"
                     /translation="MLNSLFGRVLILIAAVVFCLLQSKLWMGDGGFLDIQQIKQEIAE
                     QQAENEKLAERNRILKAEVNDLKSGTEAIEEHARLDLGLVKTDETFILVTANKQPVVS
                     TPPAVEGPTP"
     gene            complement(8612..9904)
                     /gene="eno"
                     /locus_tag="PLY05_01695"
     CDS             complement(8612..9904)
                     /gene="eno"
                     /locus_tag="PLY05_01695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNC02044.1"
                     /translation="MSQIIDIRAREILDSRGNPTIEADVYLESGVMGRGCAPSGASTG
                     SREALELRDKDGKRYLGKGVLNAVANVNSRISELLVGRSVFDQSEIDRAMIALDGTEN
                     KSNLGANAMLAVSLACARAAAVEKKVPLYEYIAGLNNQTIFTMPVPMMNIINGGEHAD
                     NTVDIQEFMIEPVGFTSFSEALRCGAEVFHALKSVLKAQGLNTAVGDEGGFAPNLRTN
                     EEALQMIMQAIDKAGYKAGEDVMLALDCASSEFYKDGRYHLEGEGKSFDSEGFAEYLS
                     GLTRQYPIISIEDGLDESDWAGWKVLTDKIGEKVQLVGDDLFVTNPKILQRGIDEGVA
                     NSILIKYNQIGTLTETLEAIYLAKNNGYTTVISHRSGETEDSTIADLAVATAAGQIKT
                     GSLCRSDRVSKYNQLLRIEQELDGLAAYRGRAEFRYAK"
     gene            complement(9976..10821)
                     /gene="kdsA"
                     /locus_tag="PLY05_01700"
     CDS             complement(9976..10821)
                     /gene="kdsA"
                     /locus_tag="PLY05_01700"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNC02045.1"
                     /translation="MTQKIIQLNNIQMGNDLPFVLFGGMNVLESRDLALRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSVTSYRGPGLDEGLKIFQEIKSTFNVPIITDVHEPYQA
                     QPVAEVCDIIQLPAFLSRQTDLVVAMAKTGAIINIKKAQFLAPQEMKHILQKCNEAGN
                     DQLILCERGSSFGYNNLVVDMLGFGTMKKMGYPVFFDVTHSLQMPGGLPNAAGGRRAQ
                     VTELARAGMAVGLAGLFLEAHPDPEQAKCDGPCALRLSQLEGFLSQVKAIDDLVKNFP
                     ELDTH"
     gene            complement(10898..>11290)
                     /locus_tag="PLY05_01705"
     CDS             complement(10898..>11290)
                     /locus_tag="PLY05_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672715.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HNC02046.1"
                     /translation="VIGLITEWVNENGEIETRNADSNLGGTMRLGAQQCHLQTSTKAR
                     AIYGQDTIVERHRHRYEVNNQYVERLTQAGLIVSGRSADNSLVEMVEIADHPWFVACQ
                     FHPEFTSSPRGGHPLFSSFIAAAMQAKK"
     assembly_gap    11291..11390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11391..12190)
                     /locus_tag="PLY05_01710"
     CDS             complement(<11391..12190)
                     /locus_tag="PLY05_01710"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNC02047.1"
                     /translation="MTKYIFVTGGVVSSLGKGISAASMAALLEARGLRVTMTKMDPYI
                     NVDPGTMSPYQHGEVFVTEDGAETDLDLGYYERFLRRAKMTQNNNFTSGRIYSDVIQK
                     ERRGDYLGATVQVIPHITDMIKHRIREGAKGYDIAIIEIGGTAGDIEAMPFMEAVRQM
                     RVELGFQGSLLMHLTLVPYIASAGETKTKPTQHSVKELRSIGLQPDILVCRSDHTISE
                     ESRSKIANSTNVELRAVILAEDAQTIYQIPRKFHEQGLDEFILERFGW"
     gene            complement(12402..13511)
                     /locus_tag="PLY05_01715"
     CDS             complement(12402..13511)
                     /locus_tag="PLY05_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC02048.1"
                     /translation="MKRNLIALAVLTAAITPLTAQANPKVYGQINLSAEAYEQDKDGS
                     VNDQEFTRMQTNASRFGVKGEAELTATLSAVYGIEWEVTAEGDDVSRLSVSTTTTVDT
                     NGDGKADSKDGKATSTSTISNGNRLDLTQRNRFVGIKHQEFGTIKMGKYDTYLKLAQG
                     KVDLFNDFAADMQFTIAGENRINNVIGYESPKLMNTQFSVMTQTQDTATKATNGNSLS
                     IVHHNEEAGLYLALAADLGINGSSALFSSRESDTIRLVGSYKIADLTANLLYTTSEKI
                     DGTDAETAYLLGTAYKLGDVVLKAQYSMAEADDTANLAATSNVEKTQATIGADYNFSS
                     KTRAFAWYTMQEETKKAAVNDVETNILAVGIETKF"
BASE COUNT         3944 a         2796 c         2525 g         4194 t
ORIGIN      
        1 cacattccaa acaagcgtga tggcggtttc tgtattggta gcaaaattgc tccaatcttc
       61 ttcaacacca tggaagatgc tggtgcattg cctgttgaat tagatgtatc acaaatgaac
      121 atgggtgatg agattgtttt aaacatcgat cacgctaatg ctattgtgac tgctactaaa
      181 gatggcgttg aaattgctaa agctccgtta aaaacacctg tgttattaga cgaagtacgt
      241 gctggtggcc gtattaactt aattatcggt cgctctttaa ccactaaagc tcgtgaggct
      301 ttaggtttag cgccttctgc tttattccgt caaccacaac aacctgctga cactggcaaa
      361 ggttataccc aagctcagaa aatggttggt aaagcgtgtg gcgtaacagg tatccgtccc
      421 ggtacgtact gcgaacccaa aatgactact gttggctctc aagataccac aggtccaatg
      481 actcgtgatg aattaaaaga tttagcttgt ttaggcttct ctgctgattt agtcatgcag
      541 tctttctgtc acaccgctgc ttatccaaag ccagttgacg ttaaaatgca ccacacttta
      601 ccagacttca ttatgaatcg tggtggtgtt gcattacgtc caggtgacgg tattatccat
      661 agctggttaa accgtatgtt gttgccagac actgttggta cgggtggtga ctctcatact
      721 cgcttcccaa ttggtatttc tttcccagca ggttctggtt tagttgcgtt tgctgcggca
      781 acaggtgtga tgcctttaga tatgcctgaa tcagtattgg ttaagtttaa aggtaaaatg
      841 caagctggta tcaccttacg tgatttagtg catgctatcc cctactatgc catccaagct
      901 ggtgatttaa cggttgagaa aaaaggcaaa aagaatattt tcaatggccg tatcttagaa
      961 attgacttgt ccgaaatgga aggcgattta acggttgaac aagcctttga attgtctgat
     1021 gcgtctgctg agcgttctgc ggctggttgt tctattacct tgtctgaaaa gtctgttgct
     1081 gaatacttaa cctctaacat caccatgtta aagtggatga tttctgaagg ttacggtgat
     1141 gcacgtacca tggctcgtcg tatcgaagca atggaaaaat ggttagccaa cccagtattg
     1201 ttgcgtgctg atgccgatgc tgaatacgct aaagtgtatg aaattgactt aaacgacatt
     1261 aaagaaccca tcctttgctg tccaaacgac ccagatgatg ccaagttgtt atccacagtg
     1321 gctggtgaca aaattgatga agtgttcatc ggttcttgta tgactaacat cggtcacttc
     1381 cgtgctgctg gtaagttgtt agaaaaagtt gaaggtggtt ctttaagcac tcgcttatgg
     1441 ttagctcctc ccaccaagat ggatgctcac cagttaatgg aagaaggcta ctacaacatt
     1501 tatggtcgcg ctggcgcacg taccgaaatg cctggttgtt ctttgtgtat gggtaaccaa
     1561 gcgcgtgttg ctccaaacac cacttgtgtg tccacttcta cccgtaactt cccgaaccgt
     1621 ttaggtcaag gctctaatgt gtatttggct tctgctgaat tagcctctgt ggctgcggtg
     1681 atgggtaaat tgccaactgt ggccgagtac atggaatatg cgaacaaaat cgacagcatg
     1741 gctggcgaca tttatcgcta tttgaacttc gaccgtatga gtgaatacac cgacaaagcc
     1801 aacaagattg atgttgctca gttgacttaa tatcaacaat taacacgact tgttaaaaca
     1861 cccccgcttt atgcgggggt tgttttttgg agaaattata taaaaatgaa ccttccactt
     1921 aaaatagttg ttatcagtga cacacatggc ctacatgact tattaaccat tcctgatggt
     1981 gatattttaa tccattgtgg tgatatgact gaatatggtc tcattgagga aattgaagag
     2041 tttgatgaat ggttaggcac attgccacac aaacataaaa ttgtcattgc aggcaatcac
     2101 gaactttgtc ttgaacaaga atcaggttgg gcattgtttc atcatgccac gtatttagaa
     2161 catgaagcgg tgaccattga gggtgtcaac ttttttggca gtccgtggca gccctatttt
     2221 catggcatgg cgtttaatct taaacgtggc gaagccttag ctaaaaaatg gcaagatatt
     2281 cctgataata ccgatatttt gattacccat agtccaccct ataaatattt ggataaagtc
     2341 cgttcacaat gggtgggttg tgccgaccta ttgcaacgag tacaacaagt caaacctgtg
     2401 tatcatgtct ttggtcatat ccatgaaagt tatggccaag aatctaatcg ccatacaact
     2461 tttattaatg cctgtatggt tgttgaatta gatgctccac tgaataaacc tatggttatt
     2521 gactggccta aacaactatt gtagtgtgat ttttgatatt aaaaacttag caatcgtcgc
     2581 gcgttctgaa gcgtcacctg catgatgaga caacagccct tccaccacaa aagtcgctat
     2641 tttggcggct gctacacact cttcttgttt taagccatgc agcattaact ggttaacaac
     2701 tagtgactcc agcataaaat gaaaacgttg atgccacgtt ttgatacttt cagcaaagtt
     2761 catgcgatta tgtacggtcg taatcacatg aggaacggca tcaaatttgt caagtaacca
     2821 caataattgc ttatctaaac tttgtgcttg aacacttaaa tattctgcta aggcttcagc
     2881 gacaagctcg tccaaaatgg caatcaataa ctcgtcttta tctttgaaat accaatataa
     2941 ggtattgggg gcaacgcctg cttctttagc caaacgagac atactggttg cgtcataacc
     3001 ttctgccaat aataagcgtt ttgccgcgaa taaaatttct tggcgtttgt gttcgctatc
     3061 aagttcacgt ctatttttgg ccataaaaat tctctatttt ttatcttgaa tagtattcaa
     3121 gcaaatgata gcattcatat tgaatgacat tcaacagacg aattaactaa tttattggga
     3181 agttttgttt tttagccatt ccaacatgtc ggagttttgc acacgctcat aaatatcttc
     3241 aactttcaaa cacaaaccaa ttgattcaaa gtaaatatca tcacctaaat aatatttaaa
     3301 tgattgccaa tggtgatttt tactcatcac ctcaatttgc acaaaatctt gttctatcag
     3361 tacatactcc aatagtgttg gaatttgcat ataagccata cgtttagtag tttcatcggc
     3421 acgtcgtgtg gatttagaaa ggacttcaac aataagtata ggcgtttcgg taaatgtgct
     3481 gtcatccgat aaattagaac aatcaaccaa gacatcagga taaaaatatt tactcgcaat
     3541 tttaacttgc atatcagaca tataaggctg gcagggctta ttttctaagt agttacctac
     3601 tttacgataa aaattaccca caatacgatt atgtgaggct ttggctcctg ccattgcata
     3661 aacttgacca tcaatatact catgtttaag atcagattgc tgctcttgct gcaagtattc
     3721 ttgctctgtc atataattgg tttgttgagg attcgccatg atttaatcct taataaatca
     3781 ccacatcaaa tcatcaggaa tttgataatc ggcgtaaggg tcttcttctg ctgtgtcggt
     3841 ggcggtcgct tcaggtttaa tattaagcac caagaccaaa ttagcatcac gagttgcaat
     3901 tttttcggca acacgtgtgg gaaccaattc ataaccattg gcaaaacgca caatcgcaat
     3961 aatgccttta attaattggt taaattgtaa gtcagtgacg tacaattttt tgattttttt
     4021 atcatcagta aattgataag caacttctcc gcctgctcga tcaattttat tggtttctat
     4081 taactgtttg atttgagcca aaatagcttt tttttcttgt tcttctttaa gctgtaagtt
     4141 taatagccta tcgcgttcta ctttctcagc catcgctttt tggacttgat ttttggcttc
     4201 gtctttagct gctttgcctg cttctaattg ctgttgatga atttttttag cacgtttagc
     4261 atcagctaaa ccacttttta agagttgggc ttttaagttt gacataataa aaaggtttat
     4321 gatttaggaa cattccctaa atcataaacc ttggttttat ctataggcaa gttaaattat
     4381 ttaatgcaat gtttgattaa atatagcatc ggccgataag ccttcacgca ttaatatttc
     4441 acgcaaacgc tttagtgcat ccacttgaat ttgacgcaca cgttcacgtg ttaaaccaat
     4501 ttgttcacct acatcctcta aggttgaagc ttcaaaacct gccaaaccaa aacgacgcat
     4561 taatacttca cgttgacgtt ctggcaacaa ggtgagccaa tgctccaagc tatggttaag
     4621 ttcattggtt tgaatctggt cagcaggatc aatagtttga ggatctgaca gcgtatctaa
     4681 taaactctta tcaccatctt cgctaaaact actgtctata gatgtgattt tttctgtcaa
     4741 tcctaacatt tgctcaacag tggcaatggg tttatctaat agattagcga tttcttcagc
     4801 agtaggttcg tgatctaatt tttggctcaa ctctcgagcg gcacgtaaat atatatttaa
     4861 ttcttttaca atatgaactg gtaaacgtac agtacgtgtt tgactcatta gtgctcgttc
     4921 aatcgtttgt cttatccacc atgttgcata cgttgaaaaa cggaaacctt tttcggggtc
     4981 aaatttttca acggcgtgta ttaagcctaa attaccttct tcaattaaat ccaaaaatga
     5041 taatccgcga ttggcataac gacgagcaat tttgacaact aagcgtaaat tgctttcaat
     5101 catacgccgt cgtgcacttt cttcaccagc tttggctaat ctagcaaaat ggacttcttc
     5161 ttctgcagat aataatggcg aaacaccaat atcatgtagg tagagttgag tagcgtcagt
     5221 tggatgtgag acactggttg ttttttttgt ttgtgctttt gatgttgcca tgatgtcgcc
     5281 ctctttataa atagagatgt atgggacacc atctctatct attgtcaacg tgggcttttc
     5341 agccctattt aattgggcaa tatgcttaat ggatcaacag gcttaccttt ataacgtatt
     5401 tcaaaatgta gtttttctct atctgcacct gttgagccta attcagcaat tttttgccct
     5461 agttgtactt tttgtccttc agttgcaatt aaacgatcat tgtgtgcgta tgcagtaata
     5521 tattcatcgc tgtggcgcac aataattaac ttaccgtacc ccactaaacc ttctcctgca
     5581 taaactacaa caccattagc agcggcatta atggcatcac cacgcttacc agcaatagca
     5641 atacctttac tacccacgcc ttcagtgcta aaccgtttaa tcagttggtt attgccatta
     5701 ttgactggcc atccccaagt cagtgaacca ctttttgcaa gtgcttgcac gttcgtaccg
     5761 acagacggtg tatttttgac gactggtttt aggtcattat tatttgtgtt aacaggcttg
     5821 gtagtgtctg tttttggttt cacgacaact ggtttattgg tcggtggcaa tggtttggca
     5881 acgattttta caggctcgct gatgcttgaa gttttttctt caggcttagc attttcgtct
     5941 tctaaaacca atttttggtc aacgtaaatg gtaaattttt catctaaatt attaattttg
     6001 cctaattctt tataatcttt atcataacgc atcgcgattg aatatagggt atcacctgct
     6061 ttaacacggt aatatggata gcttaatggt ggcaatggct gcttttcggt tagcttagct
     6121 ttttcagcat cagtcggtaa ttttttgggc gcagtactac aagctgccaa taaggcaaca
     6181 attaatgtag taaatatcca ttttgatttt tgtgcaacac gcattatcaa tatctcttca
     6241 aaatcattgg gtattcttgg tcgctaacca tgcgtgcaac gcaggtaagg catcatgact
     6301 ggttaaatct gtttgtaagg gggttaagct aacataacca tttgctacgg tttgaaaatc
     6361 tgtttcatgt tctgtaggaa caggatcacc cattaaacca atccaataaa caggtcgctg
     6421 ccgtgggtca tgactttttt ggatagatag accacgctgt cgtttgccta gttgagttaa
     6481 gcgaaaacct ttaatttgat tagcaggaat atcaggtaca ttcatattaa taatattatg
     6541 ttctggtaat gctaaatcat gccaatgact gactaaatca acgactagtt ccgctgcgac
     6601 ggcaaaagca tctacgctag tatgttgttt gacttttggc ccagccaaag aaacagccaa
     6661 tgcgggtttt cccaaaaatc gaccttcaag agccgcgcct actgtccctg aataaatcac
     6721 atcatcacct aaatttgcgc ctgaattaat accagtgacc acaaaatcag gttcatcatc
     6781 agcaaaaaaa ccatttagag ccaaataaac acagtctgca ggtgtgccat taaccgacca
     6841 aaaaccatta tctagttgag tggcacgtaa gggacgatct aaagttaaag cactcgaata
     6901 accactacgc tctgcttcag gagccatgac agtgactttg gcaattttac ttaatgcttt
     6961 agctaatgcg gccaagccgg gtgcaaatac accatcgtca ttacttaata atatgtgcat
     7021 attaatcttg ccaatcttct ttaggaatta aacataccac ggcatgagct tcaatgccct
     7081 cttggcgtcc aacaaaaccc aatttttcag tggtcgttgc tttaatactg acattttcaa
     7141 ccgcagtggc taaatcttcg gcaatccgtt gttgcattaa agcaatatga ggtgctaatt
     7201 tgggtttttc tgccaaaatg gttaaatcac aattaccgag ttgataacct tttgcttcaa
     7261 tcaaggcaaa tacacgacgc aataacaccc gactatctgc atttttataa gccatatctg
     7321 tgtcaggaaa atgcttgcca atatcgccta atgctaatgc acctaataaa gcgtcacata
     7381 aggcatgtaa agcaacatca ccatctgaat gagcaattaa tccttgatga tgcgcaattt
     7441 ttacgccgcc caatgtcaca ctgtcgccat cgccaaaacg atgaacatca atgccatgac
     7501 ctatacgtgg atatttcatg ttgtttgttg ccttattaga aactctgcta ataacaaatc
     7561 ttcaggataa gtgattttta gattatctaa tgcaccatta atcaaacgtg gtttaaaacc
     7621 taataactca accgctgatg cttcatcagt caccatattt tgctgagcta aagcatgact
     7681 aagtgcttga tagataatat caaatctaaa catttgcggg gtaaaagctt gccataattg
     7741 ttgacgaggt actgttgcaa taatgtgatt ttggccatca tcagatgctt gtttgatggt
     7801 gtcacgcaca ggtgctgcca atatgccacc tacctcatca tcaagcaatt gtgtaaacat
     7861 gttctgaata tccgtcaaac gtacacaagg acgagccaca tcatgcacca atacccaatc
     7921 atctggctga gcttcatttt taatagcttc taatcctgcc aaaacggagt ctgcacgttc
     7981 tgcgccccct gtgacaaaac gaattttttg gggatattga taagtcagtt tttttgcgat
     8041 gtcatcttca ggatgaatag gaacaataat ttgtttgatt gctgctaatg ttgacaaacg
     8101 atcaagcgtg ctttgcatca ccgttttttg ccctaagcgt aaatattgtt taggtaattg
     8161 accaccaaaa cgttttcctt tacctgcggc tgggacaatc agccaataat tcatggtgta
     8221 ggcccttcaa cagctggtgg tgtgctgaca acgggctgtt tattagctgt gactaaaata
     8281 aaagtttcat cggttttgac taagcccaaa tctaaacgtg cgtgttcttc aatcgcttca
     8341 gtaccacttt ttaaatcgtt aacttcagct tttaaaatac gattacgctc agctaatttt
     8401 tcattttctg cttgttgttc ggcaatttct tgtttaattt gttgaatatc taaaaaacca
     8461 ccatcaccca tccacaattt actttgtaat aggcaaaaca ccacagcggc aattagaatc
     8521 aatactcgac caaacaaaga atttaacata tttcttagtt cacaaattat taagggcgat
     8581 aattaattat cgcccttgct tgataaagtg attacttagc gtaacgaaac tctgctctgc
     8641 cacgataagc agctaaacca tctaactctt gctcgatacg taacaattgg ttgtatttag
     8701 aaacacggtc agaacggcac aaagaacccg ttttaatttg gccagctgcc gtagcaacgg
     8761 ctaaatcagc aatggttgag tcttccgttt cgccagagcg gtgagaaata acggtggtat
     8821 aaccgttgtt tttcgctaaa taaatggctt ccaatgtttc tgtcaatgta ccaatttggt
     8881 tatatttgat taagatggag ttagcaacac cttcatcaat accacgttgt aaaatcttcg
     8941 gattggtcac aaataagtca tcaccgacta attggacttt ctcaccaatt ttgtctgtta
     9001 aaactttcca accagcccag tcagattcat ctaaaccatc ttcgatggag ataatgggat
     9061 attgacgtgt taaaccagat aagtattcag caaaaccttc agagtcaaaa gacttgcctt
     9121 caccttctaa atggtaacgg ccatctttat aaaactcact agacgcacag tctaaagcca
     9181 acatgacatc ttcaccagct ttatagccag ccttgtcaat ggcttgcata atcatttgca
     9241 aagcttcttc gttagtacgc aagttagggg caaaaccacc ttcatcaccc acggctgtat
     9301 ttaaaccttg tgcttttaac acgcttttta aagcatggaa aacttctgcg ccacaacgta
     9361 aggcttcaga aaaagacgta aatccaacag gctcaatcat aaactcttga atatcaacgg
     9421 tattatctgc atgctcacca ccattgataa tattcatcat gggaactggc atagtgaaga
     9481 tagtttggtt gtttaagcca gcaatgtatt catacaaagg cacttttttc tcaacagccg
     9541 cagcacgtgc acaggctaaa gaaacggcca acatggcatt tgcacctaaa ttgcttttgt
     9601 tttctgtacc atctaaagca atcatggcac ggtcaatttc gctttgatcg aatacagaac
     9661 gaccaactaa taactcacta atacggctat tcacgttagc aactgcgttt aatacgcctt
     9721 tccctaaata acgtttacca tctttatcgc gtaattctaa ggcttcacga gagccagtcg
     9781 atgcaccact aggtgcacaa ccacgtccca tcacaccaga ttctaaatat acatcagctt
     9841 caatggttgg gttgccacga gaatctaaaa tttcacgggc gcgaatatcg ataatttggc
     9901 tcattatcca aactccttaa ggttaataat taataagatg tttacagtta aaaacaactt
     9961 attaatcaat aggtattaat gtgtatctaa ttcagggaaa ttttttacta agtcatcaat
    10021 cgctttgact tgacttaaaa agccttcaag ttgtgataaa cgtaaggcac aaggaccatc
    10081 acatttggct tgttctggat caggatgtgc ttctaaaaat aagcctgcta aaccaactgc
    10141 catacctgca cgtgctaatt ctgtcacttg tgcacgacga ccacctgctg cattgggcaa
    10201 accacccggc atttgtaacg aatgagtgac atcaaaaaag acgggatagc ccattttttt
    10261 catggtgcca aagccaagca tatcaacgac taaattgtta taaccaaagc tactgccacg
    10321 ttcacataaa atgagttgat cattacctgc ttcattacat ttttgcagga tatgtttcat
    10381 ttcttgtggg gctaagaatt gcgctttttt gatattaata atcgcacctg ttttagccat
    10441 cgccacaact aaatcggttt ggcgtgataa aaatgcaggt aattgaataa tatcgcaaac
    10501 ttcggcaaca ggttgtgctt ggtaaggttc gtgaacgtcg gtgataatgg gcacattaaa
    10561 ggtgcttttg atttcttgaa aaatctttaa accttcatct aaaccaggtc cacggtaaga
    10621 agtgactgat gaacgattgg ctttatcaaa agaggcttta aacacatagg gaatgcctaa
    10681 cttttgagtg accgtgacat aatgttcaca aatacgcaat gctaaatcac gagattctaa
    10741 aacattcatg ccaccaaaca agacaaacgg taaatcattg cccatttgaa tattattcaa
    10801 ttgaataatt ttttgtgtca tcgttatctc cttgcttcat tatgactctt actcactaag
    10861 tgagtaagag tttagcccta agtttagggc tatgttatta ttttttagct tgcatagctg
    10921 ccgcaataaa gctactgaac aaaggatggc caccacgcgg agagcttgta aactctgggt
    10981 ggaattgaca agccacaaac caaggatggt ctgcaatttc aaccatttcg accaaactat
    11041 tatccgcact acggccagac acaattaagc ccgcttgtgt taaacgctca acatattgat
    11101 tgttgacttc ataacggtgg cgatgacgtt caacaatggt atcttgacca taaatagcac
    11161 gtgctttggt actcgtttgc aaatgacact gttgtgcccc taagcgcatc gtaccgccta
    11221 aattagaatc tgcattacgc gtttcgattt caccgttttc attcacccat tcggtaatca
    11281 aaccaatcac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttccaaccaa
    11401 aacgctctaa aataaattcg tctaagcctt gctcatggaa tttacgggga atttggtaaa
    11461 tggtttgcgc atcttcggct aaaattaccg cacgtaattc aacattagtc gaattagcaa
    11521 ttttagaacg agattcttca gaaattgtat ggtcagagcg acatactaaa atatcaggtt
    11581 gcaaaccaat cgaacgcagt tctttcaccg aatgttgtgt tggtttggtt ttggtttcgc
    11641 ctgcggaagc aatataaggc actaaggtta agtgcattaa taaagagcct tgaaagccta
    11701 actcaacacg catctggcgt actgcttcca taaaaggcat ggcttcgatg tcgcctgctg
    11761 taccaccaat ttcaataatg gcaatatcgt agccttttgc gccttcacga atacgatgtt
    11821 taatcatatc ggtgatatga ggaataactt gaaccgttgc gcctaaataa tcaccacgac
    11881 gttctttttg gataacatca gagtaaatac ggccactggt aaagttatta ttttgcgtca
    11941 ttttagcacg acgcaaaaaa cgctcgtaat agcctaagtc aaggtcggtt tctgcaccat
    12001 cttcggtcac aaaaacttca ccatgctgat aaggactcat tgtaccaggg tcaacattaa
    12061 tataaggatc catcttggtc attgtgaccc gtaagccacg cgcctctagt aaagcggcca
    12121 ttgatgcggc agaaatacct ttaccgagtg aagaaacaac accacctgtg acaaaaatat
    12181 atttagtcat gaaaaaccta gactgcttat gcgatagagg gttgaattag gctaagaatt
    12241 ggctaaaaaa tagcaacaat tagcaaagcc taaaaataat ggtgcggatt ctatcaggac
    12301 aagcgttttg tctcaataaa ataaaaaacc cgcgtaatgc gggttatatt aaaaaacatc
    12361 catgtatagg cataatccat tgcctaaaaa tatcttccta attaaaactt agtttcaata
    12421 ccaaccgcta aaatgttagt ttcaacatca ttaacagcag cttttttggt ttcttcttgc
    12481 atggtgtacc aagcaaatgc acgagtttta gaactgaagt tgtagtcagc accaatagtg
    12541 gcttgtgttt tttcgacgtt agatgtagct gctaaattag cagtatcgtc tgcttctgcc
    12601 atgctgtatt gtgcttttaa aacaacatca cctaatttat aggcagtacc taataaataa
    12661 gcggtttctg catctgtgcc atcaattttt tcactggtgg tataaagcaa gtttgctgtt
    12721 aaatcagcaa ttttgtagga gcctaccaaa cgaatggtat cactttcacg actgctaaat
    12781 aatgcagagc tgccattaat acctaaatca gcagctaacg ctaaatataa gccagcttct
    12841 tcattatgat gaacaataga taaagaattg ccgttagttg ctttagtagc cgtatcttga
    12901 gtttgcgtca tcacactaaa ttgtgtattc atcagttttg gtgattcata accaatcaca
    12961 ttattgatac ggttttcacc tgcaatagta aattgcatat cggctgcgaa atcattaaac
    13021 aaatccactt tgccttgggc tagttttaaa taggtatcat atttacccat tttgattgta
    13081 ccaaactcct gatgtttgat acctacaaaa cgattacgtt gtgttaagtc taaacgatta
    13141 ccattagaga tggtcgatgt tgaggttgcc ttaccatctt tactgtcagc tttaccatca
    13201 ccatttgtat cgacagttgt tgttgtgctt acagacaagc gactaacatc atctccttct
    13261 gcggtaactt cccattcaat accataaaca gcacttaatg ttgcggttaa ttcagcctcg
    13321 ccttttacac caaagcgaga ggcatttgtt tgcatacgag taaattcttg atcgttaaca
    13381 gaaccatcct tgtcttgttc ataagcttca gcagataaat tgatttgacc atatactttt
    13441 ggattagctt gtgctgttaa tggagtaata gcagcagtta acacagctaa ggcaataaga
    13501 ttacgtttca tgggatgagt ctcctcgacc tagttttaaa aacagacgta caaatcaaa
//
LOCUS       DAOQMB010000020        13113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1785_length_13113_cov_11.920355, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000020 DAOQMB010000000
VERSION     DAOQMB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13113
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1785_length_13113_cov_11.920355"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLY05_01720"
     CDS             <1..575
                     /locus_tag="PLY05_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC02049.1"
                     /translation="NGAPSALVLGDNIFYGHEFSKLLQDANAKTAGASVFAYHVQDPE
                     RYGVVEFDANGRAISIEEKPKQPKSNYAVTGLYFYDEKVVEIAKSIKPSARGELEITD
                     VNSAYLQEGTLDVQTMGRGYAWLDTGTHESLLEASQFIATIEARQGLKVACPEEIAFR
                     SRWIDAEQLSRLAEPLKKNAYGQYLLSMLK"
     gene            591..1142
                     /gene="rfbC"
                     /locus_tag="PLY05_01725"
     CDS             591..1142
                     /gene="rfbC"
                     /locus_tag="PLY05_01725"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNC02050.1"
                     /translation="MKIIDTAISDVKIIEPAVFGDERGFFYESFNQAKFEAAIGYSVD
                     FVQDNHSRSVKGVLRGLHYQLPPHAQAKLVRCVVGEVFDVAVDIRRSSPTYGKSVGVH
                     LSAENKRQLWIPEGFAHGFLTLSDSAEFLYKTTNYYAPQSERSILWSDPELAINWPKI
                     DMDFQLSHKDKNGELFSTAEVFE"
     gene            1139..1966
                     /locus_tag="PLY05_01730"
     CDS             1139..1966
                     /locus_tag="PLY05_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC02051.1"
                     /translation="MNPHASQSSSLLFLLQSLIQHRQLILQMIQREVMGRYKGSLLGV
                     TWSFFYPILMLAVYTFVFSVVFKARWVDGTGSKTEFALVLFAGLMVFNLFSECITRAP
                     SLIISNINYVKKVVFPLEIFPIVVLGAALFHFFISFFVWLIFYCIFLGVPHFTILLMP
                     IALLPMILMILGLSWFLASLGVYFRDITQIIGVVVTVLMFLSPIFYPIAALPEIYRPF
                     MQISPLTFVVEQARDLMMWNKSLDVQALSMALLYSFIVAWLGFVWFQKTRKGFADVI"
     gene            1956..3278
                     /locus_tag="PLY05_01735"
     CDS             1956..3278
                     /locus_tag="PLY05_01735"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC02052.1"
                     /translation="MSSNIAISVSNVSKCFQMYTNPRDRLLQFFSSGKRQYFKEFWAL
                     KNITIDVLQGETVGILGCNGSGKSTLLQIIAGTLSPTSGEVCINGVLAALLELGAGFN
                     PEFTGRENVYLNGAVLGLTQEQMDRRFEEIVNFADIGTHLDQPVKTYSSGMFVRLAFA
                     VQACVEPNILIVDEALSVGDEKFQRKCFDYIEKLRMNGCAILLVTHSTATVEKFCQRA
                     ILLHKGELHGFGEANVIVDQYHALLYSDEKAYLRYLRNQKVTSETVLEEEHSTGTIQI
                     DMPNLQPIVSQSEQEGSVSLRAVIKSWEILDEFANYSETFRVGEEGRIRFLVDVFDSI
                     SEIQAGVLIRTVEGVSAFGTSTLYHKNNYINAKPNTTLQFDFHLNFALCTGTYFITLA
                     IAEAISSNDMIYLDRKTDVIVVKMSEVRPLGTGIAALKSSVNVSEINR"
     gene            3278..6097
                     /locus_tag="PLY05_01740"
     CDS             3278..6097
                     /locus_tag="PLY05_01740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC02053.1"
                     /translation="MNSILIVTSIFDIGGLETHIKGQINELTALGCKVHLATGSQFNS
                     VLVPKSVASVTKNLAFDSNATINEVLYTIETLRQLIRKHKIDIVHAHPFASILLGCIA
                     AEMEGVAFTLTLHGPASLNSLSYFQEFLFKSCLLPNAKLIFGVSEEVEELVTPYLRDK
                     NQISVLSNGIDFSSFKLSVKNQIDHRWLIVSRLDSMKIVGITDFIIKAKQVGLKGVLI
                     VGDGPAKEALQNQLLLDGLGEFVEFLGMRVDVPQLMQTVAGVAGMGRVALEGVASFKP
                     VFLIGYDGVKGVLNPKLFELACKANFSGRNLVTIDVNHFQTQYEYINNDEIKKLYNDV
                     SNEFSESVVWRKFLEKIQIIPPQSSSILSDFYRLLRVNHFPNDSFWSSSVIFDCFGSL
                     IHSMKYFEQNMVASFFYYRDKFIDIRLVQEISERDEKIVYLTQEISIGREENVCLVQE
                     ISERDEKITHLIQEHDFLEKRILENVNKIQALEQSFIQIKSSKSWLLTRPLRFTRMFV
                     NDPMVALYQLAKFIYWRLPLPVRQSLHGVKDKVIRQFKGRMITQAVGDTSDLSWEEFN
                     SGILANRDSYLGIFIQELAVDWNIPLYQRPQHMATALGRLGYLVIYKTDNWGGDNVNG
                     FREVEKNVWLTNKTQQVDTIPSVVRSIYSTAFQNTPQTLLKNNKRGILIYEYIDHIDP
                     QISGDAENIQRLLALKDLACTSDVDFVVASARKLEEEMVSLVGKKKVISIPNGVDTRH
                     YRNPIHKKMALPNNVVQFRKKYTNIVGYFGALAPWLWYDLINDLVKSRPDLGFVFIGP
                     DYYGGLAKLPKAENVLYLGVVDYKILPAYAHTFDVCFIPFALGEIAKTTSPLKLFEYF
                     ALMKPVVVTSDMQECIQFQEVFSGGDVSSISQAIDKAIVIKKDHEFCQKLSNLADQND
                     WDKRAQELDVVFQALKDGILSENRN"
     gene            6138..7238
                     /gene="wecB"
                     /locus_tag="PLY05_01745"
     CDS             6138..7238
                     /gene="wecB"
                     /locus_tag="PLY05_01745"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HNC02054.1"
                     /translation="MKKRVLCVVGTRPEAIKMAPVILALKNEKKIDVRVLATAQHRQM
                     LDQVLSFFDIQPDIDLDLMQPNQSLTLLTSRLLNKLDDVLLIEKPDVVLVQGDTTTVM
                     TVSLACFYHRIPIGHIEAGLRTWDIYNPFPEEANRVITGRLAKWHFAPTQSSKENLLK
                     EGVVENSIFVTGNTVIDALLMTAAKDITLDVSIPENQRMILITSHRRENFGQPFREIC
                     AAIKYLANQNLDVFFLYPVHPNPNVKDVAYEILGDCKNICLCEPLDYAPFVAAMKRSY
                     FIISDSGGVQEEAPALSKPVLVLREETERPEAVDLGVVRLLGANYDKIIEVAQSLLDN
                     EEVYRAMAKGISPYGDGHSASRIAGVLVEYFS"
     gene            7238..8347
                     /locus_tag="PLY05_01750"
     CDS             7238..8347
                     /locus_tag="PLY05_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02055.1"
                     /translation="MNLVYFSPVPWASFAQRPHKFVEQFHANTGGKVLWIDPYPARLP
                     TFADFARIKVRHNMHNLSENIPSWLTPIYPKAFPIEPLRGFNKTNNLFWSDIFVKIDK
                     WLQREDTLIGVGKPSDLACQVLAKYRNRFSFYDAMDDFPEFHKGLSRISMARYEQKVL
                     SLVSKVLVSSTALMKRFQKQYRDVTFVPNACSPELFPSIDSVFVLKKQPLIVGYVGMI
                     YHWFDWGIVFKLAQENPSFLFKLIGPLCEPPPGQLLTNMELCPPCDHPTAIAAMAEFS
                     VGLIPFKLNQLTDSVDPVKYYEYRALNLPVISTAFGEMRSHADNDSRVFLIDDQANSA
                     EVLKQALNTRNSSDEINQFRLQNSWEKRFDVSGIW"
     gene            8396..9793
                     /gene="wbaP"
                     /locus_tag="PLY05_01755"
     CDS             8396..9793
                     /gene="wbaP"
                     /locus_tag="PLY05_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HNC02056.1"
                     /translation="MFFTKKNLALIDIYVLFIAFILGQLPRLHEFSWSLNTWWHLEGQ
                     FRIYGVALVLVGFLFTFGFIYQHYTERKPFWDELRESIKTLSLLMILDAAFFFFNKIH
                     FSRVAFAIQWFAIFCLLPTFRTLVKLYALKIGKWQIPVRLIGNGENAKEAWLALKSEG
                     LMGYELAEVVQTSHVEQDDSWITVPQLSWQEGLKPRQNIQVVVALEAEQQTELDEVVR
                     VLSRFYPNMIIVPPTRGLPLWGMQPLHSFSHEVLLLRAQNSLLRPSARLIKRAFDVFV
                     SLTLLLLLSPLFAYLFYKVKQTEGDAFFGHQRIGANGNPFPCYKFRTMVKNSAEVLDE
                     LLKNDAQAKAEWEAEFKLKNDPRITQIGHVLRRTSLDELPQLWNVLRGEMSLVGPRPV
                     VQAELIKYGDDVDYYLHVKPGMTGLWQVSGRNDVDYETRVALDAWYVRNWSLWNDLVI
                     LLKTVKVVLARDGAY"
     gene            complement(9770..10891)
                     /locus_tag="PLY05_01760"
     CDS             complement(9770..10891)
                     /locus_tag="PLY05_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004649890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC02057.1"
                     /translation="MKKLTVIQTLPALNAGGVERGTLEIARALVAAGHRSIVISNGGR
                     LVEQLEAEGSEHITLPVHRKSLASLWQIAPFKQILLDIKPDIIHARSRIPAWIAWLAW
                     RKLPLSNRPHFVTTVHGFYSVSFYSAIMTKGERVIAVSNSVKDYITQNYKHCPINNIQ
                     VIHRGVSQQEFPHGYQPNQQWLSQWQQTYPQLQGKKVLTLAGRITRLKGHETFIQLIK
                     QLIADGENVHGLIVGGAEAKKQAYLQEIEQLIIKENLSQHITMTGHRSDIKEVFSQSD
                     LIYSLSSQAETFGRTVLEALYLGKSVLGWNHGGVGEILAACYPQGSLNLNDTVSLLNK
                     TKDLLHSPQTPPIINQFRLDDMCGQTLKVYEDLVSTVTC"
     gene            complement(11007..12011)
                     /gene="lpxL"
                     /locus_tag="PLY05_01765"
     CDS             complement(11007..12011)
                     /gene="lpxL"
                     /locus_tag="PLY05_01765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005107954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferase"
                     /protein_id="HNC02058.1"
                     /translation="MRLNCRSPFYDLRRFMKETPFTWHFLHPRYWPIWLLLIPLLFVF
                     SKLPWGIQRRIGTFLGLLVYSVIGQRRRDTATNIRLCFPEASEHEQAMMVKDVFIQGG
                     ISIFETANAWFKPKEYYHKKVTIQGLHHLQAAKAEGKGILLLGAHYSFVDLCGLLAII
                     FFPVDTVYRPQNNKALEYFMVKRRLRIYDYQIDHDNMRLLIKALKENHTVWYTPDQDF
                     GLKQGVFAPFFGMQAATVTAPRRLQKINNSAVMLVHFRRHPVLEQYEILITPRLENYP
                     SDDPVADATLVNHHLEQLIRRSPTQYMWFHRRFKTRPEGEAMPYPKKRRHLKEEQGNI
                     "
     gene            12129..>13113
                     /gene="waaA"
                     /locus_tag="PLY05_01770"
     CDS             12129..>13113
                     /gene="waaA"
                     /locus_tag="PLY05_01770"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF004388.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HNC02059.1"
                     /translation="MTSWRYNFLLHLLLPFVYLRLLWRGRTTPAYAQNIEQRFGGHKK
                     LPQGGIVIHAVSLGETLASQPLVNALLTQYPNLPLIITNTTATGAERARALWGDKVHQ
                     CYLPYDYSWAMRRFLEHSRPKLIIVMETELWPNLIDQAKQLTIPVMLANGRLSAKSAA
                     GYGKILSLVTPMLQALTVLAAQDQDTAQRFQDLGTPESTIQVTGSLKFDLTIPDDLTL
                     RADELKQQWLLQTRPIWVAASTHEGEDEIVLTAFKQIKQQFNNALLILVPRHPERFDK
                     VADLIAKQGLSIARRSQQQAITTEVSVFLGDSMGELLLWFALADVAFVGGSL"
BASE COUNT         3964 a         2026 c         2801 g         4322 t
ORIGIN      
        1 taaatggcgc gcctagtgct ttagtattag gcgataatat tttttatggt cacgagtttt
       61 ctaaattact gcaagatgct aatgcaaaaa cagcaggtgc gagtgttttt gcgtatcatg
      121 ttcaagatcc agagcgttat ggtgtagtgg aatttgatgc taatgggcgt gcaatcagta
      181 ttgaagaaaa gcccaagcaa cccaaatcta attatgcagt aacaggattg tatttttatg
      241 atgagaaagt ggttgaaata gctaaatcga ttaaaccctc tgcccgtggt gagttagaaa
      301 ttactgatgt caactcggcg tatttgcagg agggtacctt agatgtgcaa acgatggggc
      361 gtggctatgc gtggttagat acaggcacgc atgaatcttt actagaagct agtcaattta
      421 tagccacaat tgaagcaaga caggggttaa aagtggcttg ccctgaagaa attgcttttc
      481 gctctagatg gattgatgct gaacaattaa gtcgtttagc agagccacta aaaaagaatg
      541 cctatggtca atatttattg tcaatgctga agtaaagagt gttttaagct gtgaaaatta
      601 tcgataccgc aattagtgac gttaagatta ttgagcctgc cgtgtttggt gatgaacggg
      661 gtttttttta cgaaagtttt aatcaagcaa aatttgaagc ggccatagga tattctgttg
      721 attttgttca agacaatcat tcacgctcag taaaaggtgt gctgcgaggt ttgcattatc
      781 agttaccgcc acatgcgcaa gctaaactgg tgcgttgtgt tgttggtgag gtgtttgatg
      841 tggccgttga tattcgtcga agctctccta cctatggtaa atcggttggt gtgcatctgt
      901 ctgcagaaaa taaacgccag ttatggattc cagaaggatt cgcgcatggt tttttaactt
      961 tatctgattc agcagagttt ttgtataaaa cgacaaatta ttatgcgcca caatctgaac
     1021 gttcgattct gtggagtgat ccagagttag cgattaactg gcctaaaata gatatggact
     1081 ttcagttatc acataaagat aaaaatgggg aattatttag tacggctgag gtttttgaat
     1141 gaatccgcat gcttcgcaat catcttcttt attattttta ttacaaagtt tgatccaaca
     1201 tcgacaatta attttacaaa tgattcaacg tgaggttatg gggcgttaca aagggtctct
     1261 tttaggtgtg acgtggtcat ttttttatcc aattttgatg ctagctgttt atacttttgt
     1321 gtttagtgtg gtctttaagg ctcgttgggt tgatggaaca ggctcaaaaa ccgagtttgc
     1381 tttagtgttg tttgcaggtt taatggtatt taatttattt tccgaatgta ttactcgcgc
     1441 accaagttta attatttcaa atataaacta tgttaaaaaa gtggtatttc cacttgaaat
     1501 ctttcctatt gtggtgttag gtgctgcact ttttcatttt tttattagtt tttttgtgtg
     1561 gttaatattt tattgtattt ttttaggggt tccacatttt actatcttgt tgatgccaat
     1621 agcattgtta cctatgattt tgatgatact tggattgagt tggtttttag catcactagg
     1681 agtttatttt cgcgatataa cgcaaattat aggtgtagtt gtaactgttt taatgtttct
     1741 ttcacctatt ttctatccta tagcagcatt acctgaaata tatagaccat ttatgcaaat
     1801 aagccctctt acgtttgtag ttgaacaagc ccgtgatcta atgatgtgga ataaaagttt
     1861 agatgtgcag gcattaagca tggctttatt gtattcattt attgtggcat ggttgggttt
     1921 tgtgtggttt cagaaaacaa gaaaagggtt tgcagatgtc atctaatata gcaatttccg
     1981 taagtaatgt ttctaaatgt tttcaaatgt acacaaatcc acgggataga ctactacagt
     2041 ttttttcatc aggtaaacgt cagtatttta aagagttttg ggcgttaaaa aacattacga
     2101 tagatgtttt acagggagag acggtaggca ttcttggttg taatgggagt ggaaaaagta
     2161 cgttacttca aataattgca ggtacattgt caccaacttc tggtgaagtt tgtataaatg
     2221 gtgtacttgc cgcactttta gaattaggag ctggttttaa tccagagttt acagggagag
     2281 agaatgtata tttgaatgga gctgttttgg gattgacaca agaacaaatg gataggcgtt
     2341 ttgaagaaat tgtaaatttt gcagatatag ggactcattt agatcagcca gttaaaacat
     2401 actcaagtgg tatgtttgta agattagcat ttgcagttca agcatgtgtt gagccaaata
     2461 ttttgatcgt tgatgaagct ttatcagttg gtgacgaaaa atttcaacga aagtgttttg
     2521 attatattga aaaattacgt atgaatggat gtgccatttt attagttaca cattctacag
     2581 caacggtaga gaaattttgt cagcgagcga ttttattgca taaaggagag ctacatggtt
     2641 ttggagaggc taatgtaata gtagatcaat atcatgcctt attatattca gatgagaaag
     2701 cctatttaag atatttaaga aatcaaaaag tgacatctga gactgtttta gaggaagaac
     2761 attctactgg tacgatccaa atcgatatgc ctaatttgca gccaatagtc agtcagtccg
     2821 agcaagaagg gagtgtatct cttagagcag tgataaagtc gtgggagatt ttagatgaat
     2881 tcgcaaatta tagtgaaaca tttagagttg gtgaggaagg aagaatacga tttttagtag
     2941 atgtctttga ttcaataagt gagatacaag caggggtact aattcgaacg gttgaggggg
     3001 tttctgcatt tggaacaagc actctttatc ataagaataa ttatataaat gcaaagccaa
     3061 acactacttt acagtttgat tttcatctaa attttgcatt atgtacggga acttatttta
     3121 ttactttagc tattgcagag gctatttctt ctaacgatat gatataccta gatagaaaaa
     3181 ctgatgttat tgttgtaaaa atgagtgaag tgcgaccatt agggacaggt atagccgccc
     3241 ttaaatcttc tgttaatgta agtgagatta atagataatg aattcaattc tgatagtgac
     3301 atcaatattt gatattggtg gattagaaac gcatataaaa ggacaaatta atgaattgac
     3361 agctttaggt tgtaaagtgc atttggcaac tggatctcag tttaattccg ttcttgtccc
     3421 taaaagtgtg gcttctgtaa caaaaaattt ggcttttgat tctaatgcta caattaatga
     3481 ggttttatat acgatcgaga cactgagaca gcttattaga aaacataaga tagatattgt
     3541 acacgcacat ccttttgctt ctatattatt aggctgtatt gctgccgaaa tggaaggagt
     3601 tgcatttact ttaacgttgc atgggcctgc atcattaaat agtttgagtt attttcaaga
     3661 atttttgttt aagtcgtgtt tgttgccaaa tgcgaagtta atttttggag tatctgaaga
     3721 ggtcgaagag ctagtaacac cttatttaag ggacaaaaat cagatttctg tattatctaa
     3781 tggtattgat ttctcatcat ttaaattgtc tgtaaaaaat caaatagacc atcgctggtt
     3841 gattgttagt cgtcttgact caatgaaaat agtgggaatt actgatttta ttattaaagc
     3901 aaagcaggtt ggattaaaag gagttttaat tgtaggtgat ggtcctgcta aagaagcttt
     3961 gcagaatcag ttactgcttg atggtttggg agagtttgta gagtttttgg gtatgagagt
     4021 tgatgttccc cagcttatgc aaactgttgc aggtgttgct ggaatggggc gtgtagcact
     4081 tgaaggggta gcgagtttta aacctgtatt tttaattggt tatgatggtg taaagggagt
     4141 tcttaatcca aaactatttg agcttgcatg caaagcaaat ttttctggaa gaaatttagt
     4201 aactattgat gtaaatcatt ttcaaacaca gtatgaatat ataaataatg atgagataaa
     4261 aaaactttat aatgatgtta gtaatgagtt ttctgaaagt gtcgtttgga ggaaattctt
     4321 agagaaaata caaataatac cgcctcaatc aagcagtata ttgagtgatt tttatcgtct
     4381 tttaagagtt aatcactttc caaatgattc attttggagt tcatctgtta tttttgactg
     4441 ttttggttca cttattcata gtatgaaata ctttgaacaa aatatggttg cgagcttttt
     4501 ttattatcga gataaattta tcgatattcg tttggtacaa gaaatatcag agcgagatga
     4561 aaaaatagta tatttaacac aggaaatatc tataggcagg gaggaaaatg tatgtttggt
     4621 acaagaaata tcagagcgag atgaaaaaat aactcactta atacaggaac atgattttct
     4681 cgaaaagaga atccttgaga atgtaaataa aattcaagca ttagagcaaa gttttattca
     4741 aattaagtct tcaaaatcgt ggttgctaac gaggccattg cggtttacaa gaatgtttgt
     4801 taatgatcca atggttgcat tatatcagtt ggctaaattt atttactggc gtttaccctt
     4861 gccagttagg cagtcgttac atggtgtaaa agataaagtg ataaggcaat ttaaaggaag
     4921 aatgattact caagctgtgg gggatacaag tgatttatca tgggaagagt ttaattcagg
     4981 tattttagca aatagagaca gttatttagg gatatttatc caagagttgg cagtggactg
     5041 gaatattcct ttatatcagc gtcctcagca tatggctaca gcactaggac gtttggggta
     5101 tttagttatc tataaaacgg ataattgggg aggagataat gttaatggat ttagagaagt
     5161 agaaaaaaat gtttggttaa caaataaaac gcaacaagtt gataccatcc catcagtagt
     5221 gagatccatt tattcaacag cgtttcaaaa tacccctcaa acactgctca aaaataataa
     5281 acgtggaatt ttaatttatg agtatatcga ccatatagat cctcaaatca gtggcgatgc
     5341 tgaaaatatc caaaggctgt tagcactaaa agatttggca tgtacgagcg atgttgattt
     5401 tgttgttgct tctgctagaa agcttgaaga agaaatggta agtctagtgg gaaagaaaaa
     5461 agtcatttca atacccaatg gagttgacac acggcattac cgtaatccaa ttcataaaaa
     5521 aatggcttta ccaaataatg tggttcagtt tagaaaaaaa tatacaaata ttgtggggta
     5581 ttttggtgct ttagcccctt ggttatggta tgacttaatt aatgatttag taaaaagtcg
     5641 gcctgatttg gggtttgtgt ttattggacc cgattattac ggtggattgg caaaattacc
     5701 aaaagcagag aatgtattat atctaggcgt tgttgattat aaaatattac cagcatatgc
     5761 gcatactttt gatgtttgtt ttatcccatt tgctttgggg gagattgcaa agactacgtc
     5821 gcctttaaag ctatttgagt attttgcatt gatgaagcct gttgtagtga cgtctgatat
     5881 gcaagaatgc atacaatttc aagaggtatt ttctggtggt gatgttagtt ctattagtca
     5941 agcgatagat aaagcgattg ttatcaaaaa agatcacgaa ttttgtcaaa aattatcgaa
     6001 tttagctgat cagaatgatt gggataagcg agcccaagaa ttagatgtgg tttttcaggc
     6061 tttgaaagat ggtattttgt ctgaaaatag aaattgaatg gtagaattgt tcagatttag
     6121 tgtattcaga gagcaatatg aagaagagag tattgtgtgt tgtaggaacg cgcccagaag
     6181 caattaagat ggctcctgtg atattagcat taaagaatga aaaaaaaatt gatgtgagag
     6241 ttctagctac tgctcaacat cgtcaaatgt tagatcaggt tttaagtttt tttgatattc
     6301 aaccagatat tgatttggat ttaatgcaac caaatcagtc tcttacgtta cttacgtcta
     6361 gattgctaaa caaattggac gatgtgttgt tgattgaaaa gcctgatgtt gttttagtgc
     6421 aaggtgatac tacaactgtt atgactgtat cgctagcatg tttttatcat cgtattccta
     6481 tcgggcatat tgaagcggga ctacgtacat gggatatata taatccgttt ccagaagagg
     6541 caaatcgtgt tattacagga agactagcta aatggcattt tgcaccaact caaagctcaa
     6601 aagaaaattt attaaaagaa ggagttgttg agaatagtat ttttgttaca ggtaatacag
     6661 tgattgacgc attacttatg acggctgcaa aagatattac attagatgtt tctattcctg
     6721 aaaatcaaag aatgattttg attacttcac ataggcgtga aaattttggg caacctttta
     6781 gagagatttg tgctgctatt aaatatttag caaatcaaaa tttggatgtc ttttttttgt
     6841 atcctgtaca tcccaatcca aatgttaagg atgttgcata tgaaatatta ggtgattgta
     6901 agaatatttg cttatgtgaa cctttagatt atgcaccatt tgtggcagca atgaagaggt
     6961 catattttat aattagtgat tctggaggag tacaggaaga ggctccagcg ttgagtaagc
     7021 ctgtcttagt tttaagagaa gaaacagaga gacctgaagc agttgattta ggagtggtga
     7081 ggttgttggg agctaattat gataaaatca tcgaagtcgc tcaatcttta cttgataacg
     7141 aagaagttta tagagcgatg gcaaaaggta tttctccata tggtgatggt cattcagcat
     7201 ctagaattgc aggtgtttta gtcgagtatt tttcttaatg aatttagttt atttttcacc
     7261 tgttccatgg gcgagttttg ctcaacgtcc acataagttt gttgagcaat ttcacgcgaa
     7321 tactggaggt aaagtattat ggattgaccc ttatccagca cgactgccta catttgctga
     7381 ttttgcacgt ataaaagtta ggcataatat gcacaatctc tcagagaata tacctagttg
     7441 gctaacacct atttatccta aagcgtttcc tatagagcct ttaaggggat ttaacaaaac
     7501 gaataattta ttttggtcag atatatttgt aaagattgat aagtggctgc aaagagaaga
     7561 tacattaatc ggtgtgggga aaccatcaga tttagcatgc caagtacttg caaagtatcg
     7621 aaatagattt tcattttatg atgcaatgga tgattttcct gagtttcata aaggcttgtc
     7681 tcgaatatca atggctcgct atgagcaaaa agtgttgtca ttggtatcaa aagttctagt
     7741 gtcatcaaca gctctaatga aaagatttca gaagcaatat cgagatgtca cattcgtacc
     7801 caatgcctgt tcaccagaat tatttccgtc tattgacagt gtttttgtat taaagaaaca
     7861 gccactcatt gtgggctatg taggtatgat ttatcactgg ttcgattggg gtatagtatt
     7921 taaattagct caagaaaacc ctagcttttt atttaaattg atagggcctc tttgtgaacc
     7981 tccaccaggc caattgttaa ctaatatgga gttatgtcca ccgtgtgatc atccaacagc
     8041 aattgcggca atggcagaat tttctgtagg cttaattcct tttaaattaa atcaattaac
     8101 agattccgtt gatccagtta agtattatga atatcgtgct ttgaatttac ctgttatttc
     8161 aacggctttt ggtgaaatgc gaagtcatgc tgacaatgat agtcgtgtat ttttgataga
     8221 tgatcaagct aactccgcag aggttttaaa gcaagctttg aatacccgaa atagttctga
     8281 tgaaataaat cagtttagac ttcaaaactc ttgggaaaaa cgctttgatg taagtggtat
     8341 ttggtaagtc aaatatctgt ttattttata tgtatgtagc cagttattca aataaatgtt
     8401 tttcacaaaa aaaaatctag cattaataga tatatatgta ttatttatcg cttttatttt
     8461 aggccaactc ccacgtctcc atgaattttc atggtcatta aacacatggt ggcatttaga
     8521 agggcaattc cgaatttatg gtgttgctct tgtgttggtt ggttttttat ttacatttgg
     8581 ttttatatat caacattaca cagagcgtaa gcctttttgg gatgaattaa gagaaagtat
     8641 aaaaacatta tcattattaa tgattttgga tgctgctttt ttctttttta ataaaatcca
     8701 tttttcacgt gttgcctttg ctattcaatg gtttgcaatt ttttgtttat tgcctacatt
     8761 ccgcactcta gttaaattat atgctttaaa aataggtaaa tggcaaattc ctgttcgcct
     8821 tattggcaat ggtgaaaatg caaaagaggc gtggttggct cttaaaagtg aaggtttgat
     8881 gggctatgag ttagcagaag tcgtgcaaac atctcatgtt gagcaagatg attcttggat
     8941 tactgttccc caactatcat ggcaagaagg attgaagccc agacaaaata tacaagttgt
     9001 tgtggcatta gaagcagagc agcaaacaga gttagatgaa gttgtgcggg ttttatctcg
     9061 tttttatccc aatatgatca ttgtaccacc aacacgtggt ttgccattat ggggtatgca
     9121 gcctttacat tcattctcgc atgaggtttt acttttacgc gcacagaata gtctattaag
     9181 accttctgcg cgtttaatta agcgagcttt tgatgttttt gtatcattaa ctttattatt
     9241 gttactgagt cctttatttg catatttatt ttataaagtc aaacaaacag agggtgatgc
     9301 attttttggt catcagcgta taggagcgaa tggtaatcct ttcccttgtt ataaatttag
     9361 aacaatggtt aaaaattcgg ctgaagtgtt agacgagtta ttaaaaaacg atgcacaagc
     9421 aaaagcggag tgggaggcag agtttaaatt aaaaaatgat ccaagaatta cacagattgg
     9481 tcatgtttta cgccgtacca gtttggatga actacctcaa ctatggaatg tgttacgtgg
     9541 tgaaatgagt ttagtcggcc ctcgtcctgt tgtgcaagct gaattgatta aatatggtga
     9601 tgatgttgat tattacttgc atgttaaacc cggtatgaca ggtttgtggc aagtgagtgg
     9661 tcgtaatgat gttgattacg aaacccgtgt tgctttagat gcatggtatg tgcgtaattg
     9721 gtcattatgg aatgatttgg tcattttact aaaaaccgtg aaggtggttt tagcacgtga
     9781 cggtgcttac taggtcttca tataccttta acgtctgccc acacatatcg tctaaacgaa
     9841 attgattgat aatagggggt gtttgagggc tatgtaataa gtctttggtt ttgtttaata
     9901 agctaacagt atcatttaaa tttaggctgc cttggggata acaggcggct aaaatctctc
     9961 caacaccacc atgattccac cccaacaccg atttgcctaa atacaaggct tctaagacgg
    10021 tacgaccaaa ggtttctgct tggctagata aagaataaat taaatcactt tggctaaaca
    10081 cttctttaat atcactacga tgaccagtca tggtgatatg ttgactcaga ttttctttaa
    10141 taatcaattg ctcaatttct tgaagatagg cctgtttttt ggcttctgca ccaccgacaa
    10201 ttaaaccatg aacattctcg ccatccgcaa tcagttgttt gattaattga ataaaggttt
    10261 catggccttt taggcgcgta atccgacccg ccaaggttaa gacttttttg ccttgtaatt
    10321 gtggataagt ttgttgccat tgacttaacc attgctgatt aggttgataa ccatgtggaa
    10381 actcttgttg gctcacacca cgatgaatca cttgaatgtt attgattgga caatgtttat
    10441 agttttgggt gatgtagtct ttaacgctat tggatactgc aatcacacgc tcgcctttgg
    10501 tcataatggc actataaaaa ctcacagaat aaaagccatg taccgtagta acaaagtgtg
    10561 ggcgattact taagggaagt ttgcgccatg ctaaccatgc aatccatgca ggaatgcgcg
    10621 agcgtgcatg aataatatcg ggtttaatat cgagtaaaat ttgtttaaat ggtgcaattt
    10681 gccataagct agctaaagat tttctatgca caggcaaggt aatgtgttcg ctaccttcgg
    10741 cttctaattg ctcaaccaat cgtccaccat tagaaatcac aatcgaacga tgtcccgcag
    10801 caaccaaggc tcgtgcaatt tcgagtgtgc cgcgctcaac cccccccgca ttgagtgcag
    10861 gcagggtttg aataacggtg agttttttca tggtgtcggt ctcgttggtg gttaagtagc
    10921 ccttcgactt cgctcaggga accattttgc gttggctgag ctaagtcaaa ttgcgttggc
    10981 tgagcgtagt cgaagccaaa aaatatttag atattccctt gttcttcttt aagatgccta
    11041 cgttttttcg ggtagggcat cgcttcaccc tcaggccgtg ttttgaagcg acgatgaaac
    11101 cacatatatt gtgtcggtga gcgtctaatt aattgttcta aatggtgatt aactaaggtc
    11161 gcatcagcaa caggatcatc actcggatag ttttctaaac gtggtgtgat taaaatttcg
    11221 tattgttcga gtacaggatg acggcggaaa tgtaccaaca tgacagccga attattaatt
    11281 ttttgcaaac gacgtggtgc agtcactgtt gccgcttgca tgccaaaaaa tggcgcaaaa
    11341 accccttgtt tgaggccaaa atcttggtca ggggtgtacc aaaccgtgtg gttttctttg
    11401 agggctttaa tcaataagcg catattgtca tggtcaattt ggtagtcata aattcgtaaa
    11461 cgacgtttaa ccataaaata ttctaaagct ttattatttt gtggccgata aaccgtgtcc
    11521 acaggaaaga aaataatcgc taataaacca cacaaatcaa caaagctata atgtgcacct
    11581 aataataaaa ttcctttgcc ttcagctttg gccgcttgta ggtgatgtaa gccctgaatg
    11641 gtgacttttt tatgataata ttctttgggt ttaaaccatg cgttagccgt ttcaaaaata
    11701 ctgatgccac cttgaataaa cacgtctttg accatcatcg cttgttcgtg ttcggaagct
    11761 tcaggaaagc ataaacgaat attggtggcg gtatcacgac gacgctgacc aatgacacta
    11821 taaactaaca atcccaaaaa tgtgccaata cgccgttgta tgccccaagg taatttgcta
    11881 aacacaaata ataaaggaat taatagcaac caaattggcc aataacgagg atgtaaaaag
    11941 tgccatgtaa agggagtttc tttcataaat cggcgtaaat cataaaaagg tgagcgacaa
    12001 ttaagcctca ttgaaactcc ctctccttgt gggagagggt tggggtgagg gcaaaattag
    12061 taaaaactag cagttatttt gccgtatctt agcgataata ccaagcggca aatatttagg
    12121 aatttagcat gacttcgtgg cgttataatt ttttattgca tctgttattg ccttttgttt
    12181 atttgcgttt gttatggcgt ggtcgaacta cccccgcata tgcccaaaat atagagcaac
    12241 gttttggtgg tcataaaaaa ttgccgcaag gtggcattgt tattcatgcg gtctctttgg
    12301 gggaaacctt agcctcacag cccttagtca atgctttatt aacacaatac cccaatttgc
    12361 cactgattat taccaatact acggcgacag gtgcagaacg cgctcgtgcc ttatggggcg
    12421 ataaagtcca tcaatgttat ttaccttatg attattcgtg ggcaatgcgt cgttttttag
    12481 agcatagtcg gcctaagtta attattgtta tggaaacgga gttatggcca aatttaattg
    12541 accaagccaa gcaactcact attcctgtca tgttagctaa tggtcgtttg tcggcaaaat
    12601 cagccgcagg ttatggcaag attctgagtt tagtcacacc catgttgcaa gccttaaccg
    12661 ttttagcggc acaagatcaa gataccgcac aacgttttca agacttaggt acgccagaaa
    12721 gtacaattca agtaacaggt agcttaaagt ttgatttaac tattcctgat gatttaacgt
    12781 tgcgtgcaga tgaattaaaa caacaatggc tgttacaaac acggcctatt tgggtggcag
    12841 caagtaccca tgaaggtgaa gatgaaatcg tgttgactgc atttaaacag attaaacaac
    12901 agtttaataa tgctttgttg attttagtgc cacgtcatcc tgaacgtttc gataaggttg
    12961 cagatttaat tgctaaacaa ggccttagca tagctcgacg cagccaacaa caagcaatta
    13021 ccaccgaagt gagtgtgttt ttaggcgata gtatgggtga gttattgctg tggtttgctt
    13081 tggcagatgt ggcgtttgtg ggagggtctt tag
//
LOCUS       DAOQMB010000021        13085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1788_length_13085_cov_17.504068, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000021 DAOQMB010000000
VERSION     DAOQMB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1788_length_13085_cov_17.504068"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLY05_01775"
     CDS             complement(<1..800)
                     /locus_tag="PLY05_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC02060.1"
                     /translation="MFASKKVGMTLILTSILAACSSTPKQPSPNTDAPAAVGSESTEL
                     DLEKLAKERAAQGLTAKKDDESKEKNVVAADSSKEPELSNVSAELQALAKPLLNDYQR
                     AVTLMKANQLDDAYTLFEELQSKAPQLAGPALNQALIQIQKQNYKEADVLLQKAIAAN
                     GKNPFAYNLQGFVYRQLGQFSKSRAAYEQALSLSPNYAKAHFNLGVLADLYLQDLPLA
                     LKHYEAYQGTQTTPDTTVAKWVIDLQKRTGTYKPPAKKAKVEEITVEET"
     gene            complement(811..3903)
                     /locus_tag="PLY05_01780"
     CDS             complement(811..3903)
                     /locus_tag="PLY05_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC02061.1"
                     /translation="MIANKTFLYRLSPLAVAIALSACSQAPVATTPMANQQAIYATPV
                     PAQAPVRTLASLDQVKVSIIQEPQFEDATERLNKIKPQSALALLTQSEQNNQPDPNSL
                     DSQINSKLEEALYNRLIQDAKKAPDASTATAYLKVARRLKTPSSETDLDKAERDMLNK
                     NIEFYKDLLERMPAEESRAEIYYELAKNYDLLTQKEESIEALKKLASEYTSTPHLTEV
                     RFRLAEDAFAKNRFTESAEYYRLVLEDKKSDFQDQALYKRAWALYRLADFETAIPLFF
                     SYAEKIWVKTKKTKQEEDSLQNALDVICLSFIQLDNGVKPLNDYFDKVGEKFYESIIY
                     QRLAKTYIDKKIYKEAAETYAAFIERHPFDPDAPGLSTAIINIYDLGGFPSLVVTAKE
                     QFVDHYDPNSAYWKQSSPEVQENLRPILEGHIVDLAKHYHATAQQTNLAADYEKAAKW
                     YRAHLSLNPKEEDAIVVNQLLAEVLYSAKNYKDAIVEFEKTAYGYSNNPKANDAAYFS
                     LLSYQEWDKSLADNAVERKALTSQRVASTLKFAEKFPQDPNTSIVMQGLIQHLYDNYK
                     DYDEVAKQLSQDIEKQVTRIVATLPQEKSSALMLQGLTNLYIGYKDYEGAVRTAKALL
                     DIQPPVDETLRLEAATVVADAQFDKGNLEEADKAYQQVLAFNIKDGKQKSKFQDKLAT
                     TYYRQAEKLRTDKNLEQASLAFQKAAESASDPKLKATADFDAADVLYKGEKYAEAIPL
                     LVAFKTKHPDNPLAQDTLEKLAFAYEKSGDLSSAALQFQAIAARDAKKNPAAAREATW
                     AAAETYEKAKQPELAIKIYQQVSTDATNPIDLRTEAYHRIYTYYDKNNMVGEAQNTLK
                     GIAQFYDKLGEKAPARVKYLGAMAHFKLAQPVYDTFAAIPLTQPLKVSLGAKKKAMQT
                     AITTYNKVASIGVAEFTTAANYQQALIYQKLSADLLASEKPKGMDELVLEQYNLVLEE
                     QAEPFNQKAIEIYKANANLVKQEVYDGFVQKSFAALAELEPGRYKKNEQIETAIDEIY
                     "
     gene            complement(3906..5804)
                     /gene="bamD"
                     /locus_tag="PLY05_01785"
     CDS             complement(3906..5804)
                     /gene="bamD"
                     /locus_tag="PLY05_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNC02062.1"
                     /translation="MENKSRFSSLTLAIALLSQSAWAVDLNKRLDVSTDTREPIFGQI
                     YYDYHMGHRFSALNTTILEKNKGMFNDEDAETESLLGDLYTEFGLPREADAALSRVQS
                     QDIPSSTRNMPWLRYGKYLFKLGNDVSTENYLRKPPANLTSFQESERRLLLANVLIRK
                     KSYAEAVGLLLDMPAQGAMASYGLYNLGIAYLQDNQTELGIFNLNEMIRLANDDEESR
                     NLKDKAALAIAYRYLQDKNFASARDSLLRVRLEGPYSNSALLSLAYSYYLNKQYQEAL
                     PALLELQRRNPADPTVQEAFLLTARTYEDLDARNQSIASYRIAAMTLREQLTQSERTA
                     IRTDEAAWPDILSPMQVNTKDADPLNIKDIPAGNNAITAGLFAKLFASPAFNEGFKTY
                     KQLARMQDLLNVRLRDINALEENAKALEARQNTLADNETKLKGLRDNYVSLVKKWQEN
                     QIRAKRIGTNADAYTEAATQAETSRLQQINKLGRKINKLNLDAKQAQELNERINRLRN
                     LTQFEVARHAASRDEIYRKLQESTEQLRETKMRLAALETLLKDNKEIIAKKNSAKFTN
                     LRTKAEQLKTQIADARLAQQTQLRELAKGLLEERRQRLTTYITETYLGMERVEGSNAS
                     PNTKASDK"
     gene            complement(5964..6491)
                     /locus_tag="PLY05_01790"
     CDS             complement(5964..6491)
                     /locus_tag="PLY05_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02063.1"
                     /translation="MNKGYSLLLAGLFAALSSTSFAAGESSLPSDLQDLKSDVLSLNR
                     DITQLENELLFPSSTTTILVGVNPGSKVRLVDVKLSIDDQIVSYHSYSEQETVALSKG
                     GLHRLFLGNVNSGSHNLQAIVTAYDADGKDFQRTVSYSFNKNSLRKIIEIKAADDSTR
                     AQPAQFNFREWESKN"
     gene            6898..8040
                     /locus_tag="PLY05_01795"
     CDS             6898..8040
                     /locus_tag="PLY05_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HNC02064.1"
                     /translation="MMNIIADENMALVQEFFGDLGNLQCYAGRQLNTQLLQQANVLLV
                     RSVTQVNQALLANTPVRFVGSATIGADHLDLRWLAQQQIMTTTAPACNARSVVEYVLA
                     VLAYLQEQLQQDMSAKVLGVVGLGNVGHLLAQVAGQLGFRVIGCDPFVQRQGVTQQPL
                     QQLLSEADIVCFHTPLTKQGEYPTYHLLAEHNLKYLKQGAILLNAGRGAVIDNVALLA
                     FLQTYPHYLSAVVLDVWENEPAINVDLVASIDIATPHIAGYSLEGKWRGTEMIYQRLC
                     QYLNQQIKHKLADFLPVNHQVLTWPHLSTIWQNYAALLRKVYPVEQDDQALRQTMCLD
                     THEARALAFDKLRKDYWPRRECSAYDLREVPIANQQQMANLGFKLC"
     gene            8207..9649
                     /locus_tag="PLY05_01800"
     CDS             8207..9649
                     /locus_tag="PLY05_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HNC02065.1"
                     /translation="MLNLFVGFYLLLSIAIGLYAARKVHSSTDYVVAGRSLPLHITTA
                     TVFATWFGSETVLGTSSTFITDGLGGIVADPFGASLCLIFVGLFFARYLYRKNLLTIA
                     DFFKQRYGRGVELATSVAIMLSYLGWVSAQMIALGLVFNVLSQGAISNDVGIVIGAAI
                     VMVYTIYGGMWSVAMTDFVQMIVIVVGMLFVGYVVTGKIDGGAVAVIEHASNAGKFDF
                     WPEPNLAAILAFIGAFVTLALGSIPQQDVFQRVMSAKDENTAVRGAVLGGSLYFIFAF
                     VPIFLGYSAFLIDEKMTLKLLETDSQLVLPTLIMEHTPILAQVMFFGALLSAIMSTAS
                     GTLLAPSTIFTENILRPILGNVSDTRFLWIIRSVVLTFGVCVTLFALNSNSSIFEMVE
                     NAYKVTLVSAFVPLVAGIYWSKANTQGAVFSMVFGVVVWIFLEATAPEGLWPPQLAGL
                     VAAIIGMLLGSLLPEMIKSPAQPPLKHTHK"
     gene            9708..10271
                     /locus_tag="PLY05_01805"
     CDS             9708..10271
                     /locus_tag="PLY05_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02066.1"
                     /translation="MKRNLSLFAIMTSLLMANYAIADEEQAPPTEQKTLTYSQKAKHI
                     MQQLKPVSVYTGVGLFSDMLNINNEAVTDLGNFYLRVGKFMESEAGAAFNMGWRYPIT
                     GGRDESGYFVGAFFGQVVASVRDGNQEYHRNGGGLDISYHWVNEHTRKTISVGLGTGF
                     SRRVDGNATEPAQPRAFFSFSTALKVF"
     gene            10456..11697
                     /locus_tag="PLY05_01810"
     CDS             10456..11697
                     /locus_tag="PLY05_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02067.1"
                     /translation="MEALQQFWQQILARPDFWGFITIPIVASVVTWLHVWMALKMVFY
                     PISFWGFKLGNLPVGWQGIVPRKAGKISGILVDQSLSKIGSLDEFFREMKPDEMAEFV
                     SEALMENIDDFIDEVMTDRNKTLWENLPNGIKRRIYDHVRKQVPGIMENLVTELTFSV
                     EELVDMRDMVVKQMENDRAMMVRMFLRVGQKEIDYIWHSSFWIGLFFGVVQMFVWLVV
                     PWHWTLVFWAALWGFLTNWIAIWMVFNPIEPKKIMGMTWHGAFMRRQAEVSEVFSHVS
                     TRELFTIRQIMNEMMFGPKADKTRKVVKRHVSKLLDTPIVRTAIQMTMGWEEYAKLKD
                     TIVDKSIAATMVPISDPDLNESRAAKVNELFLERMKKLTPMEFQNLLRPAFREDEWIL
                     IVLGGVTGGLAGWIQLMLGFK"
     gene            complement(11755..12345)
                     /locus_tag="PLY05_01815"
     CDS             complement(11755..12345)
                     /locus_tag="PLY05_01815"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HNC02068.1"
                     /translation="MNTALNEQALNQLFTEARTFSAWQDKAVSSELLHQLYDLVKFAP
                     TAANTTPARFVFVSSPEAKAKLKPALAEGNIDKTMQAPVTVIVAADYQFYEHLPVLFP
                     HTDAKSWFVGNQAAIDSTAFRNSSLQGAYLIMAARALGLDAGPMSGFNNQLVDELFFA
                     GTQIKSNFLINLGYGDVAKLHTRNPRLSFEQAAHIL"
     gene            complement(12396..>13085)
                     /locus_tag="PLY05_01820"
     CDS             complement(12396..>13085)
                     /locus_tag="PLY05_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNC02069.1"
                     /translation="FMLALHSSAQRGQGNYGWLEARYSFSFASWYDPRFMGVSHLRVI
                     NEDKVRPLTGFATHSHSNMEILTYVLAGEVSHKDSMGNTTKISAGEFQLMSAGKGVSH
                     SEMNSHPTQTLHLMQIWLVPAEQNTEPRYQQMQFADSQQLALIASPIEQEGSMHIKQD
                     AAIYRLKFNQGEKISLPIDRQYGYFHLIEGQGVINDVGLTTGDALISKDELALHFTAL
                     SNGQALWFDLS"
BASE COUNT         3704 a         2249 c         2724 g         4408 t
ORIGIN      
        1 gtttcttcta ccgtaatttc ttcaacttta gctttttttg caggaggctt gtaagtgcca
       61 gtccgttttt gcaagtcaat gacccatttt gctactgttg tatcaggtgt tgtttgtgtg
      121 ccttggtatg cttcataatg ttttaaagcc agtggcaagt cttgcaaata taagtctgct
      181 aagacaccta agttaaaatg agctttggca taattagggc ttaaactaag agcttgctca
      241 taggcagcac gagatttact aaattgacct aattggcgat aaacaaagcc ttgtagattg
      301 taagcaaacg gatttttgcc attcgctgca attgcttttt gcaataacac atccgcttct
      361 ttgtagtttt gcttttgaat ctgtattaag gcctgattaa gtgcagggcc agcaagttga
      421 ggtgctttgc tttgaagctc ttcaaacagc gtataagcat catctaactg attggctttc
      481 attaatgtta ctgctcgttg ataatcattg agcagtggtt ttgcgagtgc ttgtaattcg
      541 gcactcacat tacttaactc aggttctttt gagctatcag cagcaacgac atttttttct
      601 ttgctttcat catctttttt ggctgttaac ccctgagcag cacgctcttt ggctaacttt
      661 tctaagtcaa gttcggtgct ttctgaccca actgccgcag gggcatcggt gttggggctt
      721 ggctgtttgg gagtagagct acaagcagct aaaatactag ttagtattaa tgtcatgcct
      781 acttttttag atgcaaacat tccttaatcc tcaataaatc tcatcaattg ctgtttcgat
      841 ttgttcgttt ttcttataac gaccaggttc gagctcagct aaagcagcaa aacttttttg
      901 gacaaaacca tcataaactt cttgtttaac aaggtttgca ttggctttat aaatttcgat
      961 ggctttttga ttaaaaggtt cagcttgttc ttcaaggaca aggttatatt gttcgagtac
     1021 caactcgtcc atgcctttcg gtttttcgct tgctaataaa tcggctgata atttttgata
     1081 aattaacgcc tgttgatagt ttgcagccgt tgtaaactca gccacaccta tacttgctac
     1141 tttgttataa gtggtaatag cggtttgcat ggcttttttc ttagctccca aactgacttt
     1201 taagggttga gttaaaggaa tggcagcaaa ggtatcataa acaggttgtg ctaacttaaa
     1261 gtgagccatt gcgcccaaat atttaactcg tgctggggct ttttcgccta acttgtcata
     1321 aaattgagca atccctttaa gcgtattttg tgcttctcca accatattgt ttttatcata
     1381 gtaagtgtaa atacgatgat aggcttcggt tctcaaatca atagggttag tcgcatcagt
     1441 gctaacttgt tgataaattt taatggccaa ttcgggttgt ttggcttttt cataggtctc
     1501 agcggcagcc catgttgctt cacgagcagc ggctgggttc tttttggcat cacgagcagc
     1561 aattgcttgg aactgtaagg cagcactgga taaatcacct gatttttcat aagcaaaagc
     1621 taatttttct aaggtgtctt gtgctaaggg attgtcgggg tgtttagttt taaaagcgac
     1681 taataacgga attgcttcag cgtatttttc acctttatag agtacatcgg cagcgtcaaa
     1741 gtcggcagtt gctttaagtt ttggatcact agccgattct gcagcttttt gaaaggctaa
     1801 agaggcttgt tctaagtttt tatcggtacg cagtttttcc gcttggcgat aataggtagt
     1861 tgctagctta tcttggaact tagatttttg tttgccatct ttaatattaa aggccaatac
     1921 ttgttgataa gctttgtcgg cttcttctaa attaccttta tcaaattggg catctgcaac
     1981 aacagtggca gcttcaagac gtaaagtttc atcaacaggt ggttgaatat ccaataaggc
     2041 tttggcagta cgcacagcac cttcataatc tttataacca atataaagat tggttaaacc
     2101 ctgtaacatc aaggcagaac ttttttcttg gggtagagtt gctacaatac gagtgacttg
     2161 tttttcaatg tcttgactga gttgtttggc aacttcgtca tagtctttat aattatcata
     2221 gagatgctga ataagacctt gcatcacaat tgacgtattt gggtcttggg ggaacttttc
     2281 tgcaaactta agagtactag caactctttg agaggttaat gctttgcgtt caacagcatt
     2341 gtcagctaaa gatttatccc attcttgata gcttaataaa gaaaaataag cggcatcatt
     2401 ggctttaggg ttgttgctat agccataagc tgttttttca aactcaacaa ttgcgtcttt
     2461 atagttttta gcagagtata aaacttctgc taataattgg ttaacaacaa tcgcatcttc
     2521 ttctttgggg ttaaggctta gatgtgcgcg ataccatttg gcggcttttt catagtcagc
     2581 agctaggtta gtttgttgtg cggtcgcatg ataatgtttg gctaagtcaa caatatggcc
     2641 ttctaaaata ggacgtaaat tttcttgtac ttctggagac gattgtttcc agtaggcact
     2701 gttaggatca taatggtcaa caaactgttc tttggctgtc accactaatg aagggaatcc
     2761 acctaggtca taaatattaa taatggcagt gcttaaacct ggtgcatctg ggtcaaaagg
     2821 atggcgttca ataaaagcag cataagtttc ggctgcttct ttataaatct ttttatcaat
     2881 ataggtttta gctaaacgtt gataaataat agattcatag aatttttcgc ctactttatc
     2941 aaagtaatca ttaagtggtt ttacgccatt atcaagttga atgaacgaca aacaaattac
     3001 gtccaacgca ttttgtaaag agtcttcttc ttgtttggtt tttttggttt tcacccaaat
     3061 tttttcggca taactgaaga aaagtggaat agccgtttca aagtcggcta aacgatataa
     3121 tgcccaagcg cgtttgtata aagcttgatc ttgaaagtct gattttttat cttctaatac
     3181 taaacgataa tattcagcag attctgtaaa gcgattttta gcaaacgcat cttcggctaa
     3241 acgaaaacgt acttcggtta aatgcggggt gctagtatac tcagaggcta actttttaag
     3301 tgcttctata gattcttctt tttgtgtcag caagtcataa tttttcgcta actcataata
     3361 aatttctgcg cgcgactctt cagcaggcat acgttctaat aagtctttat aaaactcaat
     3421 atttttattg agcatatcgc gttcagcttt atcaaggtct gtttcgctgc tgggggtttt
     3481 aaggcggcgt gctactttta aataagcagt tgcagtgctc gcatcaggcg cttttttagc
     3541 atcttgaatt aagcgattat ataatgcttc ttctaatttg ctgttaattt gagagtctaa
     3601 gctatttggg tcaggctgat tattttgctc gctttgagtt aacaatgcta aagcagattg
     3661 tggcttgatt ttgtttaaac gctcagtcgc atcttcaaat tgaggttctt gaataataga
     3721 cacttttact tggtcaagac ttgctaaggt gcgaactggt gcttgagctg gcactggtgt
     3781 tgcgtaaata gcttgttgat tggccatagg tgttgtagca acaggtgctt gactacaagc
     3841 acttaacgct attgctactg ctaaaggact aagccgataa agaaaagttt tattagcaat
     3901 catatttatt tatcacttgc tttggtgttg ggggaggcat tggagccttc aacacgttcc
     3961 attccaagat aagtttcggt gatataggtt gttaaacgtt gacgacgctc ttctaacaga
     4021 cctttcgcta attcacgtaa ctgtgtttgt tgagcaagac gggcatctgc tatttgtgtt
     4081 tttagctgtt ctgctttcgt tcgcaaatta gtaaattttg cactattttt ctttgcgata
     4141 atttctttat tatcttttag caaggtttct agtgctgcaa gacgcatttt ggtttcacga
     4201 agttgttcgg ttgactcttg caatttgcga taaatttcat cacgagacgc tgcatgacgt
     4261 gctacttcaa actgagtcag attgcgtaaa cgattaatac gttcattcaa ctcttgagct
     4321 tgtttggcat caaggttcag tttattaatc ttacgaccta gtttattaat ttgctgtaaa
     4381 cgagaagttt cggcttgagt tgctgcttct gtataagcat ctgcatttgt acctattcgt
     4441 ttggcacgaa tttgattttc ttgccatttt ttaactagtg atacatagtt atcacgtaag
     4501 ccttttaatt tggtttcatt gtcagcaagc gtattttgtc gtgcttctaa ggctttagca
     4561 ttttcctcta aggcattaat gtcgcgtaaa cgaacattta ataagtcttg catacgagca
     4621 agttgcttat aggttttaaa accttcatta aaagcagggg aggcaaatag cttggcaaat
     4681 agtcctgctg taattgcatt attgcctgca ggaatatctt taatatttaa aggatcagca
     4741 tctttggtat tgacttgcat gggacttaaa atatcaggcc atgcggcttc gtctgtgcga
     4801 atggctgttc tttctgattg tgtaagttgt tcacgcaagg tcatggcagc aatacggtag
     4861 ctggcaattg attgattacg cgcatctaaa tcttcgtaag tccgtgccgt caataaaaaa
     4921 gcttcttgta cggttggatc agcaggattt cggcgttgta attctaataa agcagggagt
     4981 gcttcttgat attgtttatt aagataatag gaatatgcca agcttagcag tgcagaatta
     5041 ctataggggc cttctaaacg aacgcgtaac aaagagtcgc gtgcagatgc gaagtttttg
     5101 tcttgtaaat aacgatatgc aatagctaaa gctgctttat cttttaaatt gcgactttct
     5161 tcatcatcat tagccaaacg aatcatttcg ttgaggttaa aaatacctaa ttctgtttgg
     5221 ttgtcttgta aataagcgat acctaaattg tataagccat aactcgccat tgcaccttga
     5281 gcaggcatat ccaacaataa accaacggct tcagcataac tttttttacg aattaaaaca
     5341 ttcgccaata ataaacgtcg ctcagattct tgaaagcttg ttaagttagc aggtggcttc
     5401 cgtaagtaat tttcagtact aacgtcatta cctaatttaa ataaatattt gccataacgt
     5461 agccatggca tattacgtgt acttgagggg atatcttgcg actgtacacg agataatgcg
     5521 gcatctgctt cgcggggtaa accaaattcg gtgtataaat cgcctaataa gctttctgtt
     5581 tcggcatctt catcattaaa catgcctttg tttttttcta agattgttgt atttaatgct
     5641 gaaaaacgat gccccatgtg atagtcatag taaatttgac caaaaatagg ttcacgagta
     5701 tcggtagaaa catctagacg tttgtttaga tcaactgccc atgcagattg acttaacaag
     5761 gctatggcca atgtaaggct agaaaatcgc gacttgtttt ccataaatcg ttttatcatc
     5821 gtcatttatc ccaatagcaa gacacttgtt acgctaacga ttttttgcgt ttaataaagt
     5881 gtcatactta aagctctttg ctaactaacg gtcttgcggt caattagcaa agttaggttt
     5941 tttagggctg aatcaatcct aatttagttt ttgctttccc attcgcggaa gttaaactgt
     6001 gcaggttgag cacgagtgct atcatctgcc gctttaattt caataatttt acgtaagcta
     6061 tttttgttaa agctatagct cactgtacgt tgaaagtctt tgccatcggc atcataggct
     6121 gtaacgatag cttggaggtt gtgactaccg ctattgacat tacctaaaaa cagacggtgt
     6181 aaaccacctt tgctcaaagc aacggtttct tgctcagagt aagagtgata actaacaatt
     6241 tggtcatcaa tactaagttt gacatcgact aaacgaactt tactaccagg attaacaccg
     6301 acaagaattg ttgtggttga acttggaaat aataattcgt tttcgagttg tgtaatatca
     6361 cgatttaatg acaacacatc tgattttaag tcctgtaaat ctgaaggtaa gctagactca
     6421 ccagcagcaa aactcgtcga agatagggca gcaaaaaggc cagccaataa taaagaatat
     6481 cccttgttca taatcatcat cagttactat gacagtaaaa tatatatttt acttaaaggt
     6541 taaaaatatt agtgctttaa taaagcactg ccgcaaagta gagatgtcta agatttgttt
     6601 aatagcgaac aaaccttaat atattacacc gaaaagcgat taacttagat taacgcataa
     6661 gcaataccct tacgtcaaag ggagttttac tgttttggcg cacagtgtac aaaaaaaatt
     6721 agagctagat agattgtcca acataaattt aagaaaagga ttgactaaaa atgccaattt
     6781 gacttgaatt agtgcctttt ttaatgtagc aaatttggca gcaataatcg agtagggctt
     6841 gttgatgttt taattggaaa actccaaaag taatctccaa attattgagt aaaaaagatg
     6901 atgaatatta ttgctgatga aaatatggct ttggtgcaag agttttttgg agacttgggg
     6961 aatttgcaat gttacgcggg acgtcaatta aatacacaac ttttacaaca agcaaatgtc
     7021 ttgctcgtgc gttcagtcac acaagtcaat caagctttat tagcgaatac gcctgtcaga
     7081 tttgttggta gtgcaacgat tggtgcagat cacttagatt taagatggtt ggcacagcag
     7141 caaattatga caacgacagc acccgcgtgt aatgcgcgta gtgttgttga atatgtattg
     7201 gcagtgttag cgtatttaca agagcaatta cagcaggata tgtcagctaa agttttaggt
     7261 gttgttggtt tgggtaatgt ggggcattta ttggcacaag tagctggcca attaggattt
     7321 cgagtcattg gctgtgatcc ttttgtgcag agacaaggtg ttactcaaca acctttacaa
     7381 caattattat cagaagcaga tattgtttgt tttcatacac cactaaccaa acaaggtgaa
     7441 tatccaacct atcatttatt agctgagcat aatttaaaat atttaaagca aggtgcaatt
     7501 ttattgaatg caggacgtgg agctgtgatt gataatgttg ctttattggc ttttttacag
     7561 acgtatccgc attatttatc agcggtggtg ttagatgttt gggaaaatga accagctatt
     7621 aatgtcgatt tagttgccag cattgatatt gctacgcctc acattgcagg atatagctta
     7681 gaaggcaaat ggcgtggtac agaaatgatt tatcaaagat tatgccaata tctcaatcaa
     7741 caaattaaac ataagttggc agattttttg cctgttaatc atcaggtgtt gacttggcca
     7801 catttatcaa caatatggca aaactatgca gcattattac gtaaggttta tcctgttgag
     7861 caagatgacc aagcattacg tcaaaccatg tgtttagata ctcatgaggc gcgtgcatta
     7921 gcttttgata agttgcgtaa agattattgg cctaggcgag agtgttcggc ttatgatttg
     7981 cgagaagttc ctatcgctaa tcaacaacaa atggcaaatt taggttttaa actgtgttga
     8041 ctttacggtg cgctattctt tcgccatcgt tatcaaaact ttaacagaca ctaatattgt
     8101 aaaaacgata aatacctaat aaaaaaacgc gacttttagc gtattagctt ttaaaatcgc
     8161 cgcattttta attaacctga tctacctaaa atgattgagg agagccatgt taaatttatt
     8221 tgttggtttc tatctgttgc tatccattgc tattggctta tatgcagcaa gaaaagtaca
     8281 cagtagtact gattatgtgg ttgcaggtcg tagtttgcct ttacacatca caacggcaac
     8341 tgtttttgca acatggttcg gctctgaaac cgttttggga acatcctcta cgtttattac
     8401 tgatggttta ggtggtattg ttgctgatcc ttttggcgca tcgctctgtt tgatttttgt
     8461 cggtttattt tttgcgcgtt atttatatcg aaaaaatttg ctgaccattg ccgatttttt
     8521 caaacaacga tatggcagag gtgttgaact agcaacatca gttgccatta tgttgtctta
     8581 tttaggttgg gtttctgcgc aaatgatcgc cttaggcttg gtgtttaatg tgttgtcgca
     8641 aggtgcgatt agtaatgatg ttggcattgt gatcggggct gccatcgtga tggtttatac
     8701 catttatggt gggatgtggt ctgtggcaat gacggacttt gtacaaatga ttgtgattgt
     8761 cgtgggaatg ttatttgtag gctatgtggt cactggcaaa attgatggtg gtgcagttgc
     8821 tgtgattgaa catgcctcta atgcaggcaa atttgatttt tggccagaac ccaatttagc
     8881 tgcgattttg gcatttattg gtgcatttgt gactttggct ttagggtcaa ttcctcagca
     8941 agatgttttt caacgggtaa tgtcggccaa agatgaaaat acggctgtac gtggtgcagt
     9001 gttgggtggg tcactctatt ttatattcgc ttttgtgcct atctttttag ggtattcagc
     9061 ttttttgatt gatgaaaaaa tgacgttaaa gttgttagaa acagatagtc aattagtatt
     9121 gccaacgtta attatggaac atacgccaat tttggcacaa gtgatgtttt ttggtgcatt
     9181 attatcggca attatgagta ctgcatcagg aacattatta gcaccatcaa ctatttttac
     9241 tgaaaatatt ttacgtccaa tattgggtaa cgtttctgat acgcgttttt tatggattat
     9301 tcgctcggtt gtgttaactt ttggcgtgtg tgtgacgcta tttgcattaa actcaaactc
     9361 ttctattttt gagatggttg aaaatgctta taaagtaacc cttgtatcag catttgtacc
     9421 tttggttgca gggatatatt ggtctaaggc aaatactcaa ggtgcggtgt tttcgatggt
     9481 ttttggtgtg gttgtatgga ttttcttaga ggcaacagcg ccagaaggat tatggcctcc
     9541 acagctagca ggtttggttg cagcaataat tggcatgtta ttaggctctt tgttgccaga
     9601 aatgataaag tcaccagccc agccaccttt aaaacataca cataaataat aataaatttg
     9661 ggctaattca tttagcccaa atctttctcc ctgtagagga cttttttatg aagcgtaatc
     9721 tttctttgtt tgcaataatg acctcattat tgatggccaa ttatgctatt gctgatgaag
     9781 aacaagctcc gcccactgag caaaaaacat taacctatag tcaaaaagca aaacatatta
     9841 tgcagcaatt aaaacctgtt tctgtgtata cgggtgttgg tttatttagc gatatgttga
     9901 atattaataa cgaagcagtc acagatctag gtaattttta tctgcgtgtt ggcaagttta
     9961 tggagtctga ggcaggtgca gcgtttaata tgggatggcg atatccaatt acaggtggtc
    10021 gtgatgagtc aggctatttt gttggagcat tttttggcca agtcgtagcg agtgttagag
    10081 atggaaatca agaatatcac cgcaatggtg gaggtttaga tattagctat cattgggtga
    10141 atgaacatac acgaaaaaca atatctgttg gtttaggaac aggtttttca agaagagtgg
    10201 atggtaatgc aactgagcca gcacaacctc gagccttttt tagctttagt acagcattaa
    10261 aagtgtttta agattgcttt cgttctcaat aagtaatatt gtcagacagt ttttttatag
    10321 ttccgtttgt gtattaaaaa atagtttcgt ttgatagttt ggggatttgc tagcttacat
    10381 tgagtaaata tgttttaaca tcaataacaa tactcttagc agctaagttc taatttgtac
    10441 aggtagtctg gattaatgga agcacttcaa caattttggc aacaaatatt agcccgccct
    10501 gatttttggg gcttcattac cattcctatt gtcgcatcag ttgttacgtg gctacatgtg
    10561 tggatggcac taaaaatggt gttttatcct attagttttt ggggatttaa actcggaaat
    10621 ttgcctgttg gttggcaagg cattgtgcca cgtaaagcag gaaaaatttc gggtattttg
    10681 gttgatcaat cattgtctaa aattggctcg ttagatgagt tctttagaga aatgaagcct
    10741 gatgaaatgg ccgaatttgt cagtgaagca ttaatggaaa acattgatga ttttattgat
    10801 gaggtgatga ctgatcgtaa taagacttta tgggaaaatt tacctaatgg cataaaacgt
    10861 cgtatttatg atcatgtacg caaacaagtg ccaggtatta tggaaaattt ggtcacagag
    10921 ttaactttta gtgttgaaga attagttgat atgcgtgaca tggtcgtcaa acaaatggaa
    10981 aatgaccgtg ccatgatggt gcgtatgttt ttgcgtgtag gccaaaaaga aattgattac
    11041 atttggcata gcagtttttg gattggtcta ttctttggcg ttgtccaaat gtttgtttgg
    11101 ctggttgtac cgtggcattg gactttagtt ttttgggctg ccttatgggg ctttttgacg
    11161 aattggattg ctatttggat ggtatttaac cccatcgaac caaagaaaat catgggtatg
    11221 acttggcatg gtgcttttat gcgtcgtcaa gctgaagtat cagaggtttt ctctcatgtt
    11281 agcactcgtg agttgtttac cattcgtcaa attatgaatg aaatgatgtt tggcccaaaa
    11341 gctgataaaa ctcgtaaagt agttaaacgt catgtgagta aattgttgga tacaccgatt
    11401 gtccgtaccg ctattcaaat gacgatgggc tgggaagagt atgcaaagtt aaaagatact
    11461 attgttgata agtctattgc tgcaacaatg gtacctattt ctgatccaga cttaaatgaa
    11521 agccgtgctg caaaagtaaa cgaattattt ttggagcgta tgaaaaaact gacgccaatg
    11581 gagtttcaaa atttattacg tcctgctttt agagaagatg agtggatttt gattgtatta
    11641 ggtggcgtca cgggtggatt ggctggttgg attcagttaa tgttagggtt taagtagttt
    11701 attgatactg tttatctgga tttttaattc agataaacag tatgagtgtc gtcattatag
    11761 gatatgagcg gcttgctcaa aacttaaccg tggattacgg gtatgaagtt tagccacatc
    11821 accataaccc aagttaatta aaaaattgga tttaatttgt gtgcctgcaa aaaatagctc
    11881 atcaactaat tgattattaa aaccagacat agggccagca tctaagccta aagcacgagc
    11941 ggccataatt aaatacgcac cttgcaaaga gctattacga aaagcggtac tgtcaatcgc
    12001 tgcttgatta ccaacaaacc atgattttgc atcagtatgt ggaaacaaaa caggtaaatg
    12061 ttcataaaac tgatagtcag ccgcaacaat cacggtaaca ggtgcttgca tagttttatc
    12121 aatatttccc tcagctaaag caggttttaa ttttgcttta gcttctgggc tgctaacaaa
    12181 gacaaaacga gcaggggtgg tattggcggc agttggggca aattttacta aatcatataa
    12241 ctgatgtaat aattcggagc taacagcttt atcttgccaa gcagaaaagg tacgtgcttc
    12301 agtaaataat tgatttaatg cttgttcatt aagtgcagta ttcatattaa attcctatat
    12361 tgtgtcgtta aataaaatat ctcaacccat gtctattaag acaaatcaaa ccataaggct
    12421 tggccattac tcagcgcagt aaagtgtaat gccaattcgt ctttgctaat taaggcatca
    12481 cctgtcgtta agccaacatc attaatcaca ccttgaccct caattaaatg aaaatatcca
    12541 tattgacgat caataggtaa actaattttt tcaccttgat tgaattttag gcgataaata
    12601 gctgcatcct gtttaatatg catagagcct tcttgttcaa taggactagc aattaaggca
    12661 agttgttggc tatcagcaaa ctgcatttgt tgataacgag gctcagtatt ttgttcagca
    12721 ggtactaacc aaatttgcat taagtgtaaa gtttgcgtag ggtggctatt catttcacta
    12781 tgactgacac ctttgcccgc actcattaat tgaaattcgc ctgcactaat tttagtggta
    12841 ttacccatgc tatctttatg gctcacttcg cctgctaaca cataggtcaa tatttccata
    12901 ttactatggc tgtgggtggc aaaacctgtg agcgggcgaa ctttatcttc gttaatcacc
    12961 cgtaagtgac taacccccat aaaacgaggg tcataccaag aagcaaacga aaaactataa
    13021 cgtgcttcta accaaccata attgccttgg ccgcgttgtg ctgaagaatg gagagctaac
    13081 ataaa
//
LOCUS       DAOQMB010000022        13084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1789_length_13084_cov_16.621537, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000022 DAOQMB010000000
VERSION     DAOQMB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13084
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_13084_cov_16.621537"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2237)
                     /gene="gyrB"
                     /locus_tag="PLY05_01825"
     CDS             complement(<1..2237)
                     /gene="gyrB"
                     /locus_tag="PLY05_01825"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNC02070.1"
                     /translation="MTDGNNYDSSSIKVLRGLDAVRKRPGMYIGDTDDGSGLHHMVFE
                     VVDNAIDEALAGYCSEVNVIIHADESITVTDDGRGIPVDIHPEEGVSAAEVIMTILHA
                     GGKFGAEDGTNSYKVSGGLHGVGVSVVNALSVRLRLVIRRNGKVYEQDYSHGEPQYPL
                     REIGETERRGTEVRFWPSAETFTNIHFSYDILAKRLRELSFLNSGVRIVLKDERTGAQ
                     DVFAYEGGLAAFVDYLNTNKTSLNKVFHFNVQTDSGIGVEVALQWNDGYQENIFCFTN
                     NIPQRDGGTHLAGFRGALTRTLNQYIESENVLKKEKVSPTGDDAREGLTAIVSVKVPD
                     PKFSSQTKDKLVSSEVRPVVESTMNERLQEYLLENPAAAKSIVAKIVEAARAREAARR
                     AREMTRRKGALDIAGLPGKLADCQEKDPALSELYIVEGDSAGGSAKQGRNRKMQAILP
                     LKGKILNVEKARFDKMLSSQEVGTLITALGCGIGREEYNPDKLRYHKIIIMTDADVDG
                     SHIRTLLLTFFFRQMPELLERGYIYIAQPPLYKVKRGKQEQYIKDDDALEVYLTDTAL
                     DDAALYVNPSAPPVLGTALAELLAEYRSVQGTIRRMSRVYPDVLLEQLLKLPALNSET
                     LGEQATVETWASKLQTVLTSAADPSQRFLVETIEDTEQHRFVVQIEMVAHGVPHTYRL
                     NYDFFASNEYRRIAQLAAKIAGLLEEGAYIKRGERVLLVSEFKQVWQWLMQESRRGNS
                     IQRYKG"
     gene            complement(2421..3497)
                     /gene="recF"
                     /locus_tag="PLY05_01830"
     CDS             complement(2421..3497)
                     /gene="recF"
                     /locus_tag="PLY05_01830"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNC02071.1"
                     /translation="MLDRLLITGVRNLRPCSLSLSRLNLFYGDNGSGKTSILESVHLL
                     AMGKSFRSHQIRKIIQHGQEQCTIFSRLNQGQQLGILKNIEGSQILKRDGQIVANMAL
                     LTYDLPVQLIDPESMALLDSGTKPRRQLLDWLVFHVEQNFFTTWQRYQRALLQRNALL
                     KKDKVDDLEWQVWEQELATNAQSLHQMRQRVFGGWLNFVQQACAILLPQYQLTIEYIV
                     GFDVEQNLQTLWAESREKDKQRGFTQIGAQRADVQIKTELGLAEAVLSRGQRKLLVCA
                     LKLAQVAYLNIYQKKCVVLLDDLSSELDKQARSRLLAMLHQLGAQMLITAVEADDIWP
                     TLLALDGQAKLFHVEQGEVLLQYG"
     gene            complement(3514..4668)
                     /gene="dnaN"
                     /locus_tag="PLY05_01835"
     CDS             complement(3514..4668)
                     /gene="dnaN"
                     /locus_tag="PLY05_01835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNC02072.1"
                     /translation="MKLSMARDQLLKPLQQVAGVVERKHTMPILANVLLRVTEDEISF
                     TGTDLEVELVATQKLTAEQFVQAGEITIPCRKLLDICKSLPVTANIELSLDENKLLIK
                     SGRSRFSLATLPAQDFPSLEEDAGAFSLNISQGNLKRLIEKTAFAMAQQDVRYYLTGM
                     LFEVTNNQLRAVTTDGHRLALFDAQAETVPTDKIQVIVPRKGVQELQRLLSDDDSALH
                     LTFGNNHLQVTLPINSAKKKDEEASVEGFVSFTSKLIDGKFPDYQRVLPRGGDKIVLV
                     SHEDFKQALQRASILTNEKYKGVRFLLKNNELCVQANNPEQEEAREELAVEYNGEELE
                     IAFNVQYLIDVLGVLQGTNTKMILSDSNASMLVQETDSQDALYVIMPMRL"
     gene            complement(4689..6020)
                     /gene="dnaA"
                     /locus_tag="PLY05_01840"
     CDS             complement(4689..6020)
                     /gene="dnaA"
                     /locus_tag="PLY05_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004914566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNC02073.1"
                     /translation="MIWTLCLERLQDEMPANLFGMWIRPLQVEESDTHLRLLAPNPFF
                     RKHIAEKYLERIRLLAADFSEGRIAQVTLEVGTKFMRAEEAPRATPPSPPPPPVVVER
                     PKPNLNEGFTFANFVQGKGNQLAYAACQAVMESPADTHHNPLFLYGATGLGKTHLMQS
                     VGHALMEKKPNARVLYLTSEKFVGGFVSALQRGAIDDFKKSCRDLDLLLIDDIHFLAG
                     KGASLEEFFYTFNTLLENNGQIILTSDRYPKEIPDLDEQLKSRFSWGLTVQLDPPDLE
                     TRVGILRKKAEVNKMDLPKPAALFIAQHIQANVRELEGALNKVIATARFQGRAIDIDI
                     VKHALKDVLAVRARQVNIDSIQKMVAEYYRIPLRELTGHKRTRIYARPRQIAMALARE
                     LTGESYPDIGAAFEGRDHTTVIHACDKVDELRKLDKEVAEDYHNLIRSLHG"
     gene            6544..6678
                     /gene="rpmH"
                     /locus_tag="PLY05_01845"
     CDS             6544..6678
                     /gene="rpmH"
                     /locus_tag="PLY05_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNC02074.1"
                     /translation="MKRTFQPSELKRKRTHGFRARMADKNGRQVLARRRAKGRKRLTV
                     "
     gene            6675..7094
                     /gene="rnpA"
                     /locus_tag="PLY05_01850"
     CDS             6675..7094
                     /gene="rnpA"
                     /locus_tag="PLY05_01850"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNC02075.1"
                     /translation="MSLVKPYLFRPEQRIRNSAGFQAVFDSPNCKVAVPQYLVLIRAN
                     QQNQSRLGFVIARKKIRFAVNRNRIKRVVRNSFRLHQYDLPAVDVVFIARQGLDELSN
                     AELAKALVKTWQQVNRRYQASIKATHENSSAHTHSNL"
     gene            7057..7263
                     /gene="yidD"
                     /locus_tag="PLY05_01855"
                     /pseudo
     CDS             7057..7263
                     /gene="yidD"
                     /locus_tag="PLY05_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005055377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            7299..8984
                     /gene="yidC"
                     /locus_tag="PLY05_01860"
     CDS             7299..8984
                     /gene="yidC"
                     /locus_tag="PLY05_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNC02076.1"
                     /translation="MQWARNLILVGLAIVSYLMILAWQKDYGTSPQPTAETAVNRSVE
                     ASASMNLPASPTAANSDVPVAPVSTDLVATAPTTPTQSFISVKTDVLDVKIDTLGGDV
                     VALSLPTYTKTVDSKEAFSLLENSRQRLFIAQSGLIGANGADARPEGRPIYQSPKVQY
                     SLVNGQSQLQVPLYFTDKNGVEIQKTFTFAQGKYDIDVSYKIINRSANPWHGLMFAQL
                     KRDLAEDPSKSHQGMGMATYLGGAWGNKEKNYNKISFDDFHDEALKETTKGGWTAIVQ
                     HYFVTAWIPDANSTNHLMTRANKDKGEYFIGFTSPDIVVNAGMQNTVNAKLYAGPKVQ
                     KELAKLSTGLELTVDYGFLWFISQFLFWVLQLLHGFLGNWGWSIVFLTLFVKLAFFHL
                     SATSYKSMANMRRVMPEMQRIKEQYGNDRQKMSMAMMELYQKEKINPVAGCLPILVQM
                     PVFLALYWTLMESVELRHAPWLLWITDLAAMDKFFVLPLIMGATMYIQQLLNPQPTDP
                     MQAKVLKLMPIIFTVFMLWFPSGLVLYWVVNNVLSIAQQWVITRQIEGAAAKK"
     gene            9113..>9528
                     /gene="trmE"
                     /locus_tag="PLY05_01865"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
     CDS             9113..>9528
                     /gene="trmE"
                     /locus_tag="PLY05_01865"
                     /gene_synonym="mnmE"
                     /gene_synonym="thdF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942564.1"
                     /note="in Escherichia coli this protein is involved in the
                     biosynthesis of the hypermodified nucleoside
                     5-methylaminomethyl-2-thiouridine, which is found in the
                     wobble position of some tRNAs and affects ribosomal
                     frameshifting; shows potassium-dependent dimerization and
                     GTP hydrolysis; also involved in regulation of
                     glutamate-dependent acid resistance and activation of
                     gadE; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNC02077.1"
                     /translation="MKLSTNNDTIAAIATPNGRGGVGIIRISGAKAVAIAMQLTQQNI
                     PFKGRYAHFKRFYAEDNAVLDEGLVLYFPNPHSFTGEDVIELQGHGGPVLMDMLLKRV
                     LDLGARMAQAGEFSQRAFLNDKLDLTQAEAIADLIDA"
     assembly_gap    9529..9628
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9629..10432
                     /locus_tag="PLY05_01870"
     CDS             <9629..10432
                     /locus_tag="PLY05_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC02078.1"
                     /translation="LRVVIAGKPNAGKSSLLNALAGYEAAIVTDIAGTTRDVLREQIH
                     IDGLPLHIIDTAGLRETGDAVEREGIRRAYHEINQADGVLLVYDLSTELSTSNQIGDN
                     LSTTGDKSDSYPQIKQKLSTDFDPLTIAKQFFDPIPPPEKLCFVANKCDLVAQEPHIS
                     RVAGYTHIILSARQNIGLEQLKTHLKALVGYQDAEHSPFIARRRHLEALKRTEIALQQ
                     AHEQLYGLALGELMAEDLRAAQEALGEITGEFTADDLLGKIFSSFCIGK"
     gene            complement(10850..11548)
                     /locus_tag="PLY05_01875"
     CDS             complement(10850..11548)
                     /locus_tag="PLY05_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02079.1"
                     /translation="MHTLISLITIRSILGVLLLSYLNLSNADDEDLKSLRPTLGVEWI
                     QVHNDKRHEIKAYIRREENKPFRSFKAEIMLNANLTTLSNVLLDFPNYHKWYWKAKDV
                     QLLKKVSPTRYLIYLIHDIPYELPDLDIILDAVIEPQTAHKNYLTIKVSALPDYLPER
                     PPLRRMSAEDMSIRITPIGKDKTLMEIQGYFEVSNTILPSMGSQYDSAFSSLHRMPTI
                     KADDQSTRIYSQKV"
     gene            complement(11560..12282)
                     /locus_tag="PLY05_01880"
     CDS             complement(11560..12282)
                     /locus_tag="PLY05_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02080.1"
                     /translation="MLKKLTICAFASLLLLPIPHAYAEPNDLDEFRSKLSNEWLLVKN
                     DRLRKIKTYARLEDGKRYRSFKVEATLDTNVESLARVLLDFENYTRWFWKTRESKLIR
                     QNSPTEYVVYMVHDAPYGLPDRDTVIKGIIEPQGKNKPYVTLRVTAIPDALPVKPPLV
                     RMVAEEMTIKFTPLPDNQVEMTTEGYFDPGGVVPAWAANFVQRNAPYAVLLALQRMTQ
                     REEYAKGKTPLPFPIYNANNLP"
     gene            complement(12378..>13084)
                     /locus_tag="PLY05_01885"
     CDS             complement(12378..>13084)
                     /locus_tag="PLY05_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02081.1"
                     /translation="IILVFSLISLSIGATAHATDDDLEHLQVKLTNQWQLIKYDKLRD
                     IKTYAKQEDGHRFRSFKVESTLNSSMKNIVQLLADFDNYKKWYWETKESKLLKTISPT
                     EHYIYMVHNSPYGLPDRDAAIRIKVIPQTPEKAQLAFLIEAVPEYLPIKKDLVRIPAE
                     DMLITLKPISANNVQLIAEGYVDPGGNFPNWANNLIQRTAPYTILVGLKRMLANNQNN
                     AKIDIPFPVYEINYYQ"
BASE COUNT         3900 a         2594 c         2449 g         4041 t
ORIGIN      
        1 cctttataac gctgaataga gttaccacga cgagactctt gcattaacca ttgccagact
       61 tgcttaaact cactgactaa cagcacacgc tcaccacgtt taatatacgc gccctcttct
      121 aacaggcctg cgatcttagc ggccaattgc gcaatacgac gatactcatt agaagcgaag
      181 aagtcataat tcaaacgata ggtatgaggc acgccatgtg cgaccatttc aatttgcacc
      241 acaaaacgat gttgttcggt atcttcaatc gtctccacca agaaacgttg gcttggatca
      301 gctgcactcg ttaacactgt ttgcaactta cttgcccatg tttcaaccgt tgcttgttca
      361 cccaaagttt cactattcag tgcaggcaac ttcaataatt gctcaagcaa tacatcggga
      421 taaacgcgac tcatacgacg aatcgtgcct tgtacactac gatactcagc caataattcc
      481 gctaaagcag tacccaatac aggtggcgca gatgggttaa catataaggc tgcatcgtct
      541 aatgcagtat ccgtgagata cacttctaaa gcatcatcat ccttaatata ttgctcttgt
      601 ttaccacgct tcactttata caacggcggt tgggcaatat aaatataacc acgttccaat
      661 aactcaggca tttgtctaaa gaagaaggtc aacaacaaag tacgaatatg tgaaccgtca
      721 acgtcagcgt cggtcatgat aataatttta tgataacgca atttgtctgg attatattcc
      781 tcacggccaa taccacatcc taacgcggta attagcgtgc caacttcttg cgaggataac
      841 atcttatcaa aacgagcttt ttcgacattc aagattttac cttttaacgg caaaatcgct
      901 tgcattttac ggttacgacc ttgcttagct gaaccacccg cagagtcacc ctcaacgata
      961 tataattcag ataatgcagg atctttttct tggcaatcgg ccaatttacc aggtaaacca
     1021 gcaatatcta aagcgccttt acgacgggtc atttcacggg cacgacgagc tgcttcacgt
     1081 gctcttgcag cttcaacaat tttggcaaca atagatttag cagccgctgg attctctaat
     1141 aaatactctt gcaaacgttc gttcatggtt gactcaacca caggacgaac ctctgaagaa
     1201 actaatttat ctttggtttg acttgagaat ttaggatctg gcactttaac cgacacaatc
     1261 gcggttaacc cttctcttgc atcatcaccc gttggcgaga ctttttcttt tttcaggaca
     1321 ttttcacttt caatatactg attaagagta cgtgttaacg cacctctaaa gcctgctaag
     1381 tgtgtaccac catcacgttg aggaatgttg ttggtaaaac aaaaaatatt ttcttggtaa
     1441 ccatcattcc actgcaaagc cacttcaacg ccaataccac tgtcagtttg tacattaaaa
     1501 tgaaatactt tatttaagct ggttttattg gtatttaaat aatcaacaaa cgccgccaag
     1561 ccaccttcgt aagcaaaaac atcttgtgcg cctgtacgct catcttttaa aacaatacgt
     1621 acgccagaat ttaaaaatga taattcacgc aaacgcttag ctaaaatatc ataggaaaaa
     1681 tggatattgg taaaggtttc tgcacttggc caaaaacgta cttctgtacc acgacgttca
     1741 gtctccccta tttcacgtaa gggatattgc ggttcaccgt gtgaataatc ctgttcataa
     1801 actttaccat tacgacgaat aaccaaacgc aaacgtactg ataaagcatt aactaccgat
     1861 acacccacgc catgcaaacc acctgatact ttataagagt ttgtgccatc ttccgcacca
     1921 aacttaccgc ccgcatgaag aatcgtcata atcacttcag cagcagacac cccttcttca
     1981 ggatgaatat caacaggaat accacgacca tcatcagtta cggtaataga ttcatccgca
     2041 tgaataatca catttacttc actacagtag cctgctaatg cttcatcaat cgcattatcg
     2101 accacctcaa aaaccatatg atgcaagcct gaaccatcgt cagtgtcacc aatatacatg
     2161 cctggacgtt tgcgtacggc atctaaacca cgcaacacct taatactaga agaatcgtaa
     2221 ttattaccgt cagtcatgtt ttttcctcaa aaaaccaata acatcaccgt gtttagagtg
     2281 atgctgtcat ctgctttact tatttaggcc ttacgcgatt atcgcttatt tggccactaa
     2341 agattctctt tcaatgtttt agctcactga gccaatcaat aaaatatcta tattcgactt
     2401 cttagataaa gacttattct ctacccatat tgcaacaaaa cttcaccttg ttccacgtga
     2461 aacaattttg cttgaccatc caatgccaat aaagttggcc atatatcgtc ggcttcaact
     2521 gccgtaatca acatttgcgc ccccaactga tgcaacattg ctaaaaggcg actacgtgct
     2581 tgtttatcta gttcagatga taaatcatcc aacaaaacca cacacttttt ctgatagata
     2641 tttaagtaag ccacttgagc caactttaat gcacacacca ataacttacg ttggcctcgt
     2701 gataataccg cttcagccaa acccaattcc gtttttattt gtacatctgc gcgttgcgct
     2761 ccaatttggg taaagcctcg ttgtttatct ttttctcgac tttcagccca aagagtctgc
     2821 aaattttgct caacatcaaa acccacaata tactctatcg ttaattgata ttgaggtaat
     2881 agtatagcac aagcctgctg tacaaaattc aaccaacccc caaatactcg ctgacgcatt
     2941 tggtgcaagc tttgcgcatt tgttgctaac tcttgctccc aaacttgcca ctctaaatca
     3001 tcaactttat ctttttttaa caaggcattt cgctgtaata atgcgcgctg ataacgctgc
     3061 catgttgtaa aaaaattttg ttccacgtga aacactagcc aatctaataa ttgtcgccgt
     3121 ggttttgtac ctgaatctaa taaagccata gattcagggt caattaattg cacaggtaaa
     3181 tcataagtaa gcagtgccat atttgcgaca atttgtccat cacgcttaag tatttgactt
     3241 ccttcaatat tttttaaaat acctagttgc tgtccctgat ttaagcgact aaatattgtg
     3301 cattgctctt ggccatgctg aataatctta cgaatttgat gactacggaa agatttcccc
     3361 atggccaata aatgtacaga ctccaagata gaggtttttc cactgccatt atcaccataa
     3421 aacaagttca atcgtgataa tgacagcgaa caaggacgca aattgcgcac ccctgttatc
     3481 aacagtctat ctaacacttt tatatactac catttatagg cgcattggca taataacata
     3541 caaagcatct tggctatctg tttcttgtac caacattgat gcattagaat cactcaagat
     3601 cattttggta tttgtgcctt gcaaaacacc taacacatca attaaatact gaacattaaa
     3661 ggctatttct aactcttcac cattatactc aacagctaat tcttcacgcg cttcttcttg
     3721 ctctgggtta ttcgcctgaa cacataactc attgtttttc aacaaaaatc ttacgccttt
     3781 atacttctca tttgtcaaaa tagaagcacg ttgtaaggct tgtttaaaat cctcatgact
     3841 aacaagtaca atcttgtcac caccacgtgg taaaacacgt tgatagtctg ggaatttgcc
     3901 atcaatcaat ttagaggtaa aagacacaaa gccctcaacc gaagcttctt catctttttt
     3961 cttggcggag ttaattggca aggtcacttg caaatggtta ttaccaaagg ttaagtgtaa
     4021 tgcgctatcg tcatcactca acaaacgctg taactcttgt acccccttac gcggcacaat
     4081 gacttgaatc ttgtctgtgg gcacggtttc agcttgggca tcaaataacg ctaagcggtg
     4141 accatcggtt gtcacagccc gtaattggtt gttggtaact tcaaacaaca tgcccgttaa
     4201 ataataacgc acatcttgtt gcgccatggc aaaggcggtt ttttcaatta aacgctttaa
     4261 attaccttgg ctaatgttta aggaaaatgc acctgcatct tcttctaaac tggggaaatc
     4321 ttgggcaggt aatgttgcca atgaaaaacg actacgcccc gatttaatca ataatttatt
     4381 ttcatctaaa cttaattcga tattggcagt gacgggtaat gatttgcaaa tatctaataa
     4441 tttacggcag ggaatagtaa tttcacctgc ttggacaaat tgctctgcgg ttaatttttg
     4501 cgtggcaact aattcaactt ctaaatccgt tcctgtaaaa gaaatttcgt cttctgtgac
     4561 acgtaataat acattcgcca aaatcggcat ggtgtgtttt ctttctacaa cacctgcaac
     4621 ttgttgtaag ggttttaata attggtctcg tgccatggat aatttcataa tgctatcctc
     4681 gtaaataatt aaccgtgtaa tgaacggatt aaattgtgat aatcttcggc gacttcttta
     4741 tccaatttac gtaattcatc gactttgtca caagcatgaa ttacggtcgt atgatcgcgt
     4801 ccctcaaagg cagccccaat atctggataa ctctcacccg ttaattcacg agctaaagcc
     4861 atggctattt gtctaggccg tgcataaata cgggttcgtt tatgtcccgt taattctcgt
     4921 aaagggattc gataatattc agcaaccatt ttctgaatac tatcgatatt cacttgccga
     4981 gcacgtacgg ctaatacgtc ttttaatgcg tgtttaacga tatcaatatc aattgcacgc
     5041 ccctgaaaac gagctgttgc aattacttta tttaatgcac cttctaattc acgaacatta
     5101 gcctgaatat gttgcgcaat aaataacgcc gcaggttttg gtaaatccat tttattaact
     5161 tcagcctttt ttcttaaaat acctacacgt gtttctaaat caggtggatc taactggaca
     5221 gttaatcccc aagaaaatcg agattttaat tgttcatcta aatctggaat ttctttagga
     5281 taacgatctg aggttaaaat aatctgtccg ttattctcta aaagggtatt aaaagtataa
     5341 aaaaactctt ctaaacttgc gcctttacct gctaaaaaat gaatatcgtc aattaataat
     5401 aaatctaaat cccgacagga ttttttaaaa tcatcaatcg caccacgttg caatgccgat
     5461 acaaaaccgc cgacaaattt ttcggaggtt aaatacaaca ctcgcgcatt tggctttttt
     5521 tccattagtg catgaccaac agattgcatc aaatgtgttt ttcctaaacc agtcgcccca
     5581 tacaaaaaca aggggttatg gtgtgtatct gcaggacttt ccatgactgc ctgacacgcc
     5641 gcataagcca actggttgcc tttaccttga acaaaattgg caaaggtaaa gccctcgtta
     5701 aggtttggtt taggtctttc caccaccact ggcggcgggg gaggggaggg gggggtagct
     5761 cttggtgctt cctcagcacg cataaacttc gtcccaacct ccaaagtcac ctgtgcaata
     5821 cggccttcac taaaatcagc cgctaataag cgaatccgct ctaagtattt ttcagcaata
     5881 tgtttacgaa aaaaaggatt tggtgccagc aaacgtaaat gtgtgtcgct ttcttcgacc
     5941 tgcagtgggc gaatccacat gccaaacaga ttcgcaggca tttcgtcttg cagacgttca
     6001 agacacagcg tccagatcat catgcgcctc caatgccgtt aaaaaggctg gtcattgtac
     6061 gcaagctgaa gtcatttgtc agctcaaaaa atagcaaaaa aaatgtaaaa aattacaaaa
     6121 acaacctgtg gataactctg tggataagtg ctgtggataa cttgcgattt tctgtggata
     6181 actatgtgga taacttttta ccccaaaagt tatccacatt ttgtccacag ggttatccac
     6241 aggtcatgta tttgttttta aatgaaaaaa gtgacttatc cacagaaaat cgcgttttcc
     6301 actataaatt ccttatttat aaatttatat ataaatttaa attctaatag taagcaacgt
     6361 gaaattctgt ggataactca aaaaactttt caggtttgtg ctaaaaaact gtggatattt
     6421 taggtttgac agaagaccat aaaatacata gaatggccgc ctttttcaga gtagccaatt
     6481 aggctattaa ccttgaacta ggactaatgc cctttcagtg ttagttcaaa cagagtaaaa
     6541 ctcatgaagc gtacttttca accaagcgaa ctcaaacgca aacgcactca cggtttccgc
     6601 gctcgtatgg cagacaaaaa cggtcgtcaa gtattggcac gtcgtcgcgc caaaggccgt
     6661 aagcgtttaa cagtgtgagc ttagttaaac cctatctttt tcggcctgaa caacgtattc
     6721 gcaatagcgc aggttttcag gccgtttttg atagtcctaa ttgcaaagtg gctgtgccac
     6781 aataccttgt gttaattcga gctaatcaac aaaaccaatc ccgtttaggc tttgtgattg
     6841 cccgaaaaaa aatccgattc gctgttaatc gtaatcgtat caaacgtgtt gtgcgaaata
     6901 gttttagatt gcatcaatac gatttacctg ccgttgatgt ggtatttatt gcacggcaag
     6961 gtttagacga gttatctaat gcagagctcg ctaaagcact cgtaaaaacg tggcagcaag
     7021 tgaatcgccg ataccaagcc tccatcaagg cgacacatga aaattcttct gctcataccc
     7081 attcgaattt ataaatatgc aatcagccct ctcatggctg gtcgttgccg tttctttcca
     7141 acctgttctg cttatgccga agaagccatt attaaacatg gtgcacttaa aggcggatat
     7201 ttagcaacaa aacgtatttt gcgttgccat ccttggggtg gtatggggta tgaccctgtg
     7261 cctgaaattt tctcttggtc aactgtggaa aaatgactat gcaatgggct cgtaacctga
     7321 ttttagtcgg cttagcaatt gtcagctatt taatgatttt agcgtggcaa aaagactatg
     7381 gtacttcccc tcaacctaca gctgaaacag ccgtaaatcg cagcgttgag gcctctgcgt
     7441 cgatgaattt gcctgcttct ccgacagcgg ctaattctga tgtgccagtt gcacctgtta
     7501 gcaccgattt ggttgcaaca gcacctacaa caccaacaca aagttttatt tctgttaaga
     7561 ctgatgtttt ggatgtcaag attgatacct tgggtggtga tgttgttgct ttgtctttgc
     7621 caacctatac caaaacggtt gatagcaaag aggcatttag tttattagaa aacagccgcc
     7681 agcgtttgtt tattgcccaa tctggtttaa ttggcgctaa tggtgcggat gcccgtccag
     7741 aaggtcgacc aatttatcaa tcgccaaagg ttcaatatag tttggtaaat ggccaaagcc
     7801 agttacaagt tcctttatat tttactgata aaaacggtgt tgagattcaa aaaacattta
     7861 cctttgcgca aggtaaatat gatattgacg tcagttataa aattattaac cgcagtgcta
     7921 atccttggca tggtttaatg tttgcacaat taaagcgtga tttagctgaa gaccccagta
     7981 aaagtcatca aggtatggga atggcgactt atttaggtgg tgcatggggt aataaagaaa
     8041 aaaactataa taaaattagt tttgatgatt ttcatgatga agccctaaaa gaaacgacta
     8101 aaggcggatg gaccgcgatt gttcagcatt attttgtcac tgcttggatc cctgatgcga
     8161 atagtacaaa tcatttaatg acgcgtgcta ataaagataa aggcgaatat tttattggct
     8221 ttacgtcacc tgatattgtg gttaatgcag gtatgcaaaa tacggtgaat gccaaattat
     8281 acgcaggacc taaagttcaa aaagaattag ctaagttatc cacaggttta gaattaacgg
     8341 ttgattatgg ctttttatgg tttatttctc agtttttatt ttgggtttta caattattac
     8401 atggcttttt gggtaactgg ggctggtcaa ttgttttctt aacattattt gttaaattag
     8461 cttttttcca tttatcggca acaagttata aatcaatggc taatatgcgt cgtgtcatgc
     8521 ctgaaatgca gcgcattaag gaacaatatg gtaatgatcg tcaaaaaatg tcgatggcaa
     8581 tgatggagtt atatcaaaag gaaaaaatta atccggttgc gggttgtttg ccgattttag
     8641 tacaaatgcc tgtattcttg gctttatatt ggacattaat ggaaagtgtc gaattacgtc
     8701 atgcgccatg gttattatgg attaccgatt tagcggcaat ggataagttt tttgtgttgc
     8761 cattaattat gggcgcaacg atgtatattc agcagctatt aaatcctcag cctactgatc
     8821 caatgcaagc taaagtatta aaattaatgc cgattatttt tacagtattt atgctgtggt
     8881 tcccatcggg cttagtttta tattgggtgg tcaacaacgt attatctatt gcacaacaat
     8941 gggtgattac acgtcaaatt gaaggcgcag cagccaaaaa ataatggcct gagtttttaa
     9001 ttattttcaa taaaaggctg ccaaataggt ggccttttat ttgggactta tgtggatatc
     9061 gcttatttcg gtaaaatgca taagtcctgt attttgattt tgggtaaaaa agatgaagtt
     9121 atccacaaac aatgacacga ttgccgctat tgccacgcca aatggtcgag gtggagttgg
     9181 tattatccgt atttctgggg ctaaggccgt tgcaatagct atgcaactta cccaacaaaa
     9241 tattccattt aaaggccgct atgcccattt taaacgtttt tatgctgagg ataatgccgt
     9301 tttagacgaa ggtttggtgt tgtattttcc caatccacat tcgtttactg gcgaagatgt
     9361 tattgaatta cagggacatg gtgggcctgt attgatggat atgttgctaa aacgggtctt
     9421 agatttaggt gctcggatgg cacaagcagg tgagttttcg caacgtgctt ttttgaatga
     9481 taaattagat ttaacccaag ctgaagccat tgcagatttg attgatgcnn nnnnnnnnnn
     9541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9601 nnnnnnnnnn nnnnnnnnnn nnnnnnnntt acgtgtggtg attgcaggta aaccgaatgc
     9661 gggtaaatca agcttactaa acgccttagc aggctatgaa gcggcaattg tgaccgatat
     9721 tgcaggaaca acacgtgatg tattacgtga gcaaattcat attgatggtt tgcctttgca
     9781 tattattgat acggcaggtt tacgtgagac gggtgatgcg gttgaacgag aaggtattcg
     9841 tcgcgcttat cacgaaatta atcaagcgga tggggtatta ttagtttatg acttatccac
     9901 agagttatcc acaagcaatc aaattgggga taacttatcc acaactgggg ataagtccga
     9961 tagttatcca cagattaagc aaaagttatc cacagacttt gatcccttaa ccattgcaaa
    10021 gcaatttttt gatcctattc ctccccctga gaaactgtgt tttgtggcaa ataaatgtga
    10081 tttagtagca caagagccgc atatttcgcg tgtagcaggt tatacccaca tcatcctatc
    10141 tgcacgtcaa aatatcggtt tagagcaact taaaacgcat ttaaaagccc ttgtaggcta
    10201 tcaagatgcc gagcatagtc cgtttattgc tcgccgtcgt catttagaag cactaaaacg
    10261 tactgaaata gccctacaac aagcgcatga gcaattgtat ggcttggctt taggtgaact
    10321 aatggcagaa gatttacgtg ctgcacaaga agctttaggt gaaattacgg gagagtttac
    10381 cgctgatgat cttttaggaa agatttttag cagcttttgt attggaaaat aatcataact
    10441 ttttggcgaa tgaccaataa tctccatata actccaaagc ctagttttac cctaaagcta
    10501 ggctttttta ttggctaaaa tttgcattaa aaatcacgaa aatcgtttga tttttgcaca
    10561 gttttattgg atgataaaat gttatattta tgattttaat agaaaaacta aaacataaag
    10621 tgatggtaat ttagatttta aaaaatattt tttgaggtaa acataagtca atttagtaga
    10681 taacttccaa aaaatcagaa gagattccaa taaaaaattt gcttattttg tattttttat
    10741 tactaattat agtgtaattt ttattacatt ttgaggcaga gttggtttat ataaaaaaag
    10801 aaggcttttt gattggtgag tttcaaattc gtaaatggga aaacctatat cagacttttt
    10861 gtgaatatat tctggtagat tggtcatccg ctttaattgt aggcatacgg tgtaaggagc
    10921 tgaacgctga atcatattgg ctgcccatgc taggtaatat agtgttgcta acctcaaaat
    10981 agccttggat ttccatgagc gttttatctt tgccaatagg tgtaatacga atactcatat
    11041 cctcggcact catacgacgt aaaggtgggc gctcaggtaa ataatcaggt aatgcagaaa
    11101 ccttaatagt aagataattt ttatgagctg tttgaggttc gataacggca tctaagataa
    11161 tatctaaatc aggaagttcg tagggaatat cgtgaataag gtaaattaaa taacgtgttg
    11221 gcgaaacttt ttttaataat tgaacgtctt tagctttcca gtaccattta tgataattag
    11281 gaaagtctaa aagaacattt gataaagttg ttagattagc attgagcata atttcggctt
    11341 taaacgagcg aaaaggtttg ttttcttcac gtctaatata agcttttatt tcgtgtcgtt
    11401 tatcgttatg aacttgtatc cattccacac ccaaagtggg tcgtagcgat tttaaatctt
    11461 catcatcagc gtttgataaa tttaaatagc ttaataacaa tacgcctaat attgaacgta
    11521 ttgttattaa tgagataagt gtatgcaaat aagcgttcat taaggaagat tattggcgtt
    11581 ataaatgggg aatggcaaag gtgttttgcc tttggcatat tcttcgcgtt gcgtcatacg
    11641 ttgtaatgct aataacacag catatggtgc attacgttgg acaaaattag ccgcccatgc
    11701 tggcactacc ccacctggat caaaataccc ttctgttgtc atttcaactt ggttatcagg
    11761 taatggcgta aacttgatag tcatttcttc ggcgaccatg cgcacgagtg gtggtttgac
    11821 aggtagggcg tcaggaatag ctgtcacgcg taaggtcaca taaggtttat ttttgccctg
    11881 aggttcaata atacctttaa ttacagtatc acgatcaggt aagccatagg gggcatcgtg
    11941 taccatatag acaacatatt cggttggaga gttttgacga atcagttttg attcacgagt
    12001 tttccaaaac caacgagtat aattttcaaa atctaacaaa acgcgagcta gtgattcaac
    12061 attggtatct aaagtagctt caactttaaa ggagcgatag cgtttgccat cttccaaacg
    12121 agcataggtt ttaattttgc gcaaacggtc atttttgact aataaccatt cgttacttaa
    12181 tttgctgcga aattcgtcta aatcattagg ttcggcataa gcatggggaa ttggtaatag
    12241 taaaagactg gcaaaagcgc agattgttaa ttttttaagc attgttgtta ttcctatggc
    12301 ttgaccatag tgtagcaaat tactcttgag aagtaatcgc tgtcaagagt atctgcaata
    12361 agctaacgaa ttgttattta ttgataatag tttatttcat agacaggaaa tggaatatct
    12421 attttggcgt tattttgatt gtttgccaac atacgtttta aacctactaa aatagtgtag
    12481 ggggctgttc gttgaattag attatttgcc caattaggaa aattaccacc tggatcgaca
    12541 taaccttcag ctattaattg tacgttatta gcactgatag gttttaacgt aatgagcata
    12601 tcttctgctg ggatgcgtac taaatctttt ttgatgggta agtactcagg aacagcttca
    12661 atcaaaaatg caagctgagc tttttctggt gtttgtggta tgactttaat tctgattgcc
    12721 gcatcacgat ctggaagtcc atagggcgaa ttatgaacca tataaatata gtgttcagtc
    12781 ggtgagatag tcttaagaag tttactttct ttggtttccc aataccattt tttgtaatta
    12841 tcaaaatcag caagtagttg cacaatgttt ttcattgaac tatttagggt actttctacc
    12901 ttaaatgatc taaaacgatg accatcttct tgtttagcgt aggttttaat gtcgcgtaat
    12961 ttatcgtatt taatgagctg ccattgatta gtcagtttga cttgtaaatg ttctaagtca
    13021 tcatcagttg catgggctgt tgcgccaata cttaggctga ttagggaaaa aactaaaata
    13081 attt
//
LOCUS       DAOQMB010000023        12848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1842_length_12848_cov_17.957555, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000023 DAOQMB010000000
VERSION     DAOQMB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12848
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1842_length_12848_cov_17.957555"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3200)
                     /locus_tag="PLY05_01890"
     CDS             complement(<1..3200)
                     /locus_tag="PLY05_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC02082.1"
                     /translation="MSLLTGKGVLAALIGCLVSGQTIADVAGRVTFVIGDVTAISSNG
                     TKRVLTKGELLNSGERLETGKGRLQIRFTDGSLLSLQPNTVLGLDNYTFSRNNPQDGS
                     LLFNFVRGGMRTISGAIGKVNRANYAVKTPAGTIGIRGTAYTATQEPNGRLLVTVNKG
                     TVNLANEFGNRNIPTGQTFQMESNKAPEPAPAGVMAEVLASQPQAVEQLVEKRETKQK
                     NRTSFSFLMPPYIPPIRPFPPDPIVPNSANSPIVPNSANSPFEFTPFFIQSVNGVPRT
                     SHFASLLKGTGESYVHENVLGAFDNVSTDGLQVGNLVGLINTPSADNGLANNALLLDT
                     RHPQTPLQIINHKQIRSLSFGEWTNGSMAFVDNLIYDKAANFSAQNFIPYVIGTASRI
                     DLGNNQQITYRLADPSHATPVRGGQNIGQLTKLNLTLDLNVQPLASLDMALTVDDINY
                     TVNAKDRFLTLSDQLSIGSFSLNGLTDDVFAVANNNSCNNQSCPVNFSAFFSANDVGA
                     LYEIIRNNNKSLSGAAILTGGTPTTIDNSIPITQRLTTSLNNNYSLLLSNNAGINNSP
                     NPFHGLSAVFNSQTGGLQLAYDAANDRINSFGSTPVNQNNTVISAATLSQIQHLDKSL
                     TWGVWSNGGIDLNNASQDFVLSNQQQVHYILGLPSAIPTSLANSRVLYSFAGGTSPSI
                     ASSNPNANLTGAIANSSYLDFDFGANTVGLNLDINISGSSTSLLNATGKTILEQNNNI
                     GSFNFTNLNIKLGNGNTCNSLGCSGNAAGFLAGAEGRLAALNYNISGVNSIVNDNFFM
                     NAQGVAAFKLNTNAALPNNILDNTDQGLYTVLLSKQLNNNPEVSTNLERVRGISANFD
                     NLTGAWQTGQIDLAGSIGQYGLNSAATNNTVSEVTHYKKTLSWGRWINSDIKLSNSPL
                     NLGANDTVHYLIGIPTAQLPTSGVVSYLHAGSTTPTGTIVTPTTVGSPANTISPLTGF
                     SVNNNSKITVDFASKANNAIKLDMNFNGGNQGNVNFQGQSALQTQFTLTNLTVNQQNC
                     QNCAATGFFAGQDASMIGVNYDVKAQLGNSTANMTGV"
     gene            complement(3218..>3880)
                     /locus_tag="PLY05_01895"
     CDS             complement(3218..>3880)
                     /locus_tag="PLY05_01895"
                     /inference="COORDINATES: protein motif:HMM:NF016462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HNC02083.1"
                     /translation="FDNNNFDYYTLDANAGLLKNIGNYSVQGKLQLQKMGLDGQAYRD
                     VIGLLTQIQKPIGETSQVAAFGQYNQLRYDTQSARDANRFTLGLAYSQQLEMRFSPTF
                     YTSIYTGQENTTDSQFDYFSHSLNGLRLGGSINYLDNLRFNSHISVEKRDYAQSNQFF
                     FPFNHIKRQDKETSVELSATWSITTKYRLQPRYNYTQNNANIPVNDYKRHVVGVDLHL
                     DF"
     assembly_gap    3881..3980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4027..>5508)
                     /locus_tag="PLY05_01900"
     CDS             complement(4027..>5508)
                     /locus_tag="PLY05_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02084.1"
                     /translation="GGLQFTNVKQIGSLSFGEWTNGSASLVDPYLGTVQQLTLTDKQF
                     MPYIVGTTAEKSLGNNMKVSYVLAGATPARAGTDIGTLNKLNIDIDLNLMPLVSVDMA
                     ANVKSITYTAKLSNYPILDISADKKFSGFILPKEELFATSSTANICANSQCPVDLSAF
                     LSGNDLGVVYQINRKNLTAINGVAALTGTESTITNNNIANPLASSLNSDYSALFSHKP
                     IITADNKTIKLTTANNLSAIFDSKTQGLMAASASIFTDTPNFYGAVGSATESAAQIQQ
                     VGHVAKVLSWGRWTNGRLATGNDDIPINLTANDNIHYLIGLPTAATAIPNVGSVTYSL
                     VGGTASAVFGTINNNQINTNFDIAQVNSGSLMVDFGRATTNLSLELNGFTKANSLQSL
                     QLSGAGTLAAGSNGFSFNNLNVSASSAQNPLVCTNCTASANGMFFGAVGSVATSNLTA
                     PTAAGVVYSINGTVQGSQATSIEANGTASFANPTPTPQNNQGS"
     assembly_gap    5509..5608
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5609..6207)
                     /locus_tag="PLY05_01905"
     CDS             complement(<5609..6207)
                     /locus_tag="PLY05_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC02085.1"
                     /translation="MNYLSTSLNGKGLLSLLIAYTLSGTALADVAGRVNFVSGNVEVI
                     ATDGSRRALVKGELVNSGERLETNRGRVQIRFTDGSFISLQPNTVFGLDKYSFDKNKP
                     NDGSLLFNFLRGGMRTVSGAIGKVNRANYKVQTPVATIGIRGTSYAANQEPNGKLLLT
                     VGSGIVNLENNFGFSNVNTGQTFQVETGQAPNQAPAGTTV"
     gene            complement(6225..7538)
                     /locus_tag="PLY05_01910"
     CDS             complement(6225..7538)
                     /locus_tag="PLY05_01910"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC02086.1"
                     /translation="MRLNAFAVALLLATNSLVWASEDILNRADALIRLQSPEQAYELL
                     APLEDELAGTPQFDYLLGLSLLEQNKPQNAIFAFERCLTVEPTNGPCRVQIARTHLAL
                     GEASNARAEFEIINEYNPPAEINDIVRGYLNAIQNLEKTQKRHLALYSQIGMGFDSNI
                     NSAPSSTNTIANTATSLDSAYISLNLGSKLLYKINPYLTSLTNINIQSRKFDKSLPDD
                     NSPYTTDIFDYQSIDANFSIQSDLESFELITQLQAQQMWLNEEEYRSIKGGLLQLQTE
                     IANGQASIFRQINTLNYDKLSTRDTKRNNTGMIYYKDIETRFTPSLFLSVYKGQEEAE
                     SNLTQYLTNSFKGIAVGSSLNITNDISLNIRMSQEKRYHNKQLFPIINIYRGDTENSM
                     NISTNWRINKHFSLIPSYNYSDNQSNIAFSDFTRHVISIDLRVDM"
     assembly_gap    7822..7921
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8292..9356
                     /locus_tag="PLY05_01915"
     CDS             8292..9356
                     /locus_tag="PLY05_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HNC02087.1"
                     /translation="MTALNPVIGRFVGSVKEDWLDFMLAHIDPMLTVKRVLAKVVAIT
                     DETQDIKRFVLQPNKHWQGFQAGQFVSVRIMINGSFHERCYSLVSAPTDSVIELGIKR
                     QPKGKVSNWMHDNLNIGDVIELGAVGGDFKLPETLPSKMLLIAGGSGVTPIYSLLVEA
                     LKRQPNADISVVYYVNTDRDLAFGNEMVELATKHRSLKLQYVLATSGDAGRFSHKQLA
                     DVCPDFTERHTYLCGPQGLMGAVTKVWAEQGISEKLTQEVFGLPAVDPNKPINEMPIT
                     LRRSQQLLLNTKPTLLESAEAGGAKPAYGCRIGMCKTCSCTKVSGVVRDVITGAIDDQ
                     PNTQIRICVTEALSPVTLDI"
     assembly_gap    9364..9463
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9496..10662
                     /locus_tag="PLY05_01920"
     CDS             9496..10662
                     /locus_tag="PLY05_01920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNC02088.1"
                     /translation="MSTISKSRDLSLEEIEAFGAELDALRKETMASLGKKEADYIYGV
                     RNFVRYTEIAGRTSLMLGWLPPFWLAGTAILGASKIVENMELGHNVMHGQYDWMNDPS
                     LRGNDYEWDTVCTADNWRHTHNFMHHTYTNVRGMDHDIGYGVLRLFPEQKWESRFLAN
                     PIAAAILAVTFEWTLALQSLELEKLISKEKTFAQVREEFKPIWAKMRKQFTKDYIFFP
                     LIAGPMFIPVITGNFFANMIRNVWAFSIIFCGHFTEDAETFPIEVLENETRGQWYLRQ
                     LHGSSNLTGNKIMHYMSGNLSHQIEHHMFPDVPGMRYAEMSVKVREICERYGQHYNTG
                     SFTKQFSTVVKRIFKYALPFSRKVEDSRAETIVAKAAKVTPITQKTATINSIAA"
     gene            10993..11364
                     /locus_tag="PLY05_01925"
     CDS             10993..11364
                     /locus_tag="PLY05_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02089.1"
                     /translation="MSKTLTRLEALKDLVQQAIDTGATSVEQIHKTIAALPLAVLEKQ
                     GLLDVDSEKRDELWDKSFGQVYEAIRRVNQEVGELATQAFEAIEDQMIIQKNIAEAEA
                     AEAAKQKIESPVAVEPKSKVV"
     assembly_gap    11369..11468
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11469..12678)
                     /locus_tag="PLY05_01930"
     CDS             complement(<11469..12678)
                     /locus_tag="PLY05_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HNC02090.1"
                     /translation="MSQYQLVVYGATSFVGQLLTRYLVQTYGVNQQLKWAIAGRSASK
                     LNELKQNLGSGASQLATIIADAQDESALKAMCQQTKVVVSTVGPYALYGSSLVKVCAT
                     LGTDYCDLTGEPQWIARMIEQYESAAQASGARIVHCCGFDSIPSDLGTFYLQQQAQQR
                     LGQTCSQVKFRVKAIRGGFSGGTVASMMELFSEISKNPSLRKVMANPYALCPPSNQQK
                     PKQPNLQFAQYDKDAEAWSAPFVMAGINTKVVHRSNALSNYAYGTQFIYDETMLTGKG
                     VIGGAMAVGASVGLAGFVAAAVIPPTRRILQKIVPQAGEGPTPKQQEQGFYDIRLIGL
                     TDNGQRLTAKVTGDRDPGYGSTAKMLGEAAVCLATDISKNDKTGGFWTPSTALGQRLI
                     ERLEAKAGLTF"
BASE COUNT         3737 a         2217 c         2500 g         3894 t
ORIGIN      
        1 acacctgtca tattggcagt actgtttcct aattgtgctt tgacatcata atttacccca
       61 atcatgctag cgtcttgccc tgcaaaaaag cctgttgccg cacagttttg gcaattttgt
      121 tgattcactg tgagattagt tagcgtaaat tgtgtttgta aggcactttg tccttgaaaa
      181 ttaacattgc cttgattgcc accattaaag ttcatatcta atttaatagc attattggct
      241 ttgctcgcaa aatcaacagt aattttacta ttattgttga ccgagaaacc tgtcagaggg
      301 ctaatggtat tagcaggaga gccaaccgtt gttggggtga caattgtgcc tgttggagtt
      361 gtgcttcctg cgtgtaaata gctcactacg ccagaagtgg ggagctgggc ggtgggaata
      421 ccaattaaat agtgaacagt atcatttgcc cctagattta acgggctatt actgagtttg
      481 atatcactat taatccatcg tccccatgat aacgtctttt tataatgtgt gacttcactg
      541 accgtattgt ttgttgcagc agaattaagg ccatattgtc caatactacc tgctaaatca
      601 atctgaccag tttgccaagc acctgttaaa ttatcaaaat tagcactaat gccacgtaca
      661 cgctctaagt tagtgctgac ttctggatta ttgttgagtt gtttgctaag tagtacggta
      721 taaagacctt ggtcagtatt atcgagtata ttatttggta aagctgcatt ggtattgagt
      781 ttaaaggctg ctacaccttg agcattcata aagaagttgt cgttaacaat gctatttaca
      841 ccagatatat tgtaatttaa agcagctaac ctgccttcag ctcctgctaa aaaaccagca
      901 gcattaccac tacaaccaag gctattacag gtgttaccat tgcccaattt gatattcaaa
      961 ttggtaaaat tgaacgaacc aatattatta ttttgttcta aaatagtttt gcccgttgca
     1021 tttaacaagc ttgtactact gccactaata ttaatgtcta aatttaatcc aacggtatta
     1081 gcaccaaagt caaagtctaa atagctactg ttagcaatag cacctgttaa atttgcatta
     1141 ggattactac tggcaataga agggcttgtt ccaccagcaa aactatataa tacgcggcta
     1201 tttgctaaac tagtcggaat agcactgggt aatcccaata tataatgcac ttgttgttgg
     1261 tttgataaga caaagtcttg gctagcatta ttaaggtcaa taccaccatt actccatacg
     1321 ccccatgtta agcttttgtc taaatgttgt atttggctta atgttgcggc tgaaatgacg
     1381 gtattatttt gattaacggg tgttgagcca aaactattga tacggtcatt agcggcatca
     1441 taagctaatt gtaaaccacc tgtttggctg ttaaaaacag cacttagccc atggaatgga
     1501 tttggactat tgttaatacc tgcattgttg cttaacaaca aggagtagtt attatttaag
     1561 ctggttgtga ggcgttgtgt gataggaata ctattatcaa tagtggtagg agtgccacct
     1621 gttaaaatag ctgcacctga taaacttttg ttattgttgc ggataatttc gtataaagcc
     1681 ccgacatcgt ttgctgagaa aaaagccgag aaattgacag gacagctttg attattacaa
     1741 ctgttattat ttgctacggc aaagacatca tctgtcagtc catttaaact aaaactacca
     1801 atactcagtt ggtctgaaag cgtcagaaaa cggtctttag cattaacggt atagttaata
     1861 tcatctacgg ttaaggccat gtctaagcta gcaagtggtt gaacattgag gtctaaagtt
     1921 aaatttaatt tagttaattg acctatattt tggccaccac gaacaggtgt ggcatggctt
     1981 gggtcagcaa gacgataggt gatttgttga ttattaccta aatctatacg actggctgtg
     2041 ccaatcacat atggaataaa gttttgtgcg ctaaagtttg cagccttgtc ataaataagg
     2101 ttatcaacaa aagccatgct gccatttgtc cattcgccaa atgataagct acgtatttgt
     2161 ttatgattaa taatttgtag tggtgtttgt ggatggcgag tatctaataa taacgcatta
     2221 ttagctaagc cattatctgc tgatggagta ttaatcagac caactaaatt accaacttgt
     2281 aggccatctg tagacacgtt atcaaaagca ccaagcacat tttcatggac ataactttcg
     2341 ccagtccctt ttaataaact ggcaaaatga ctggtacgtg gtacaccatt cacactttgt
     2401 ataaaaaatg gtgtaaattc aaaaggtgaa tttgctgaat tagggactat aggtgaattt
     2461 gctgaattag ggactattgg gtctggtgga aatgggcgaa ttggaggaat atatggtggc
     2521 atcaagaaag aaaacgatgt acgatttttc tgctttgttt ctcttttttc tactagttgt
     2581 tcaactgctt gcggctggct ggctaacact tcggccatta cccctgctgg tgcaggctct
     2641 ggggctttgt tggactccat ttgaaaagtt tggcctgttg gaatattacg attaccaaac
     2701 tcgttagcaa gattaaccgt acctttatta actgtaacca ataaacggcc attaggctct
     2761 tgggtggcgg tgtaggctgt acctctaata ccaatcgtac ctgctggtgt tttgactgca
     2821 tagttagcac gattaacttt gccaattgcg ccactaatag tacgcatccc accgcggaca
     2881 aaattaaata ataatgaacc atcttggggg ttgttgcgtg agaaagtata gttatctaaa
     2941 cctaatacgg tattaggttg caaggataac aaactaccgt ctgtaaaacg aatttgtaaa
     3001 cggcctttgc ctgtttctaa acgttcacca ctatttaata attcaccttt ggtaagcaca
     3061 cgttttgtgc cattagaact aatagcagtg acatcaccaa tgacaaaagt gactcgacct
     3121 gcaacgtctg cgatggtttg gcctgagact aaacaaccaa tcaaagccgc taagacccct
     3181 ttacctgtta aaagtgacat gatttagcta ctccctgtta aaagtctagg tgtaaatcta
     3241 cgccgacaac gtggcgttta taatcgttga cgggtatgtt ggcgttattt tgagtatagt
     3301 tataacgagg ttgtaggcga tatttggtgg taatagacca tgttgcactt aactcaacac
     3361 tggtttcttt atcttggcgt tttatgtgat taaatgggaa aaaaaattgg ttactttggg
     3421 cataatcacg tttttcaaca ctaatatggc tattaaaacg taagttatct agataattaa
     3481 tacttccacc taaacgtaaa ccattaagtg agtgactaaa atagtcaaat tgactgtcag
     3541 tcgtattttc ttggcctgta taaatgctgg tataaaaagt tgggctaaag cgcatctcta
     3601 attgttgaga ataagcgagt ccaagtgtaa aacgattggc atcacgagcc gactgtgtat
     3661 cataacgtag ttgattatat tggccaaaag ccgcaacttg gctagtttcg ccaataggct
     3721 tttgtatttg agtgagtaaa cctatcacat cacgataagc ttggccatct aagcccattt
     3781 tttgtagttg taacttgcct tgtacagagt aattgccaat attttttaat aatccagcat
     3841 ttgcatcaag ggtgtaataa tcaaagttat tattatcaaa nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn attaaaacat caacagcctc ttagactatt gcagcatcag
     4021 agattattaa ctaccttggt tgttttgtgg ggttggcgtc gggttagcaa aactagctgt
     4081 accattggct tcgatggatg tggcttggct accttgtact gtaccattaa tactatatac
     4141 aacgcctgcg gctgttggtg ctgttagatt gctagttgct acactaccaa cagcaccaaa
     4201 aaacatacca ttggcactag ctgtacaatt agtacagact aatgggtttt gagctgagct
     4261 tgcagaaaca tttagattat taaagctaaa accattgctc ccagcagcta atgtacctgc
     4321 tccgctgagt tgtaaggatt gcaagctatt ggctttagta aaaccattaa gttctaaact
     4381 taagttggta gttgcacgac caaaatcaac cattaaacta ccgctattta cttgtgctat
     4441 atcaaagtta gtatttattt ggttattgtt gattgttcca aaaacagcac ttgctgtacc
     4501 accaacgagg ctataagtga ctgatccaac attaggaatt gctgtagcag cggttggtaa
     4561 accaattaaa tagtgaatgt tatcatttgc agttaaattg attggaatat catcgttgcc
     4621 tgtagctaag cgaccatttg tccaacgacc ccatgacaag actttggcaa cgtggccaac
     4681 ttgttgaatt tgcgcagcac tttctgttgc cgaaccaact gcaccataaa aattaggggt
     4741 gtcggtaaaa atagaggcag aagcagccat taaaccttgt gttttactgt caaaaatagc
     4801 agataaatta ttggctgttg ttaatttaat ggttttgtta tctgcggtaa taatcggttt
     4861 gtgtgaaaat aaagcggaat aatcactgtt tagagatgaa gctaagggat tagcgatatt
     4921 attgtttgta atagtgcttt cagtacctgt taatgcggct acgccattaa ttgctgttaa
     4981 atttttgcga ttaatttggt aaacaacacc taagtcattg cctgataaaa aggcagataa
     5041 atcaacaggg cattgagagt tagcacaaat gttggcagta ctgcttgttg caaataattc
     5101 ttctttgggc aaaataaaac cactgaattt tttatcagca ctaatatcta aaataggata
     5161 attagaaagt tttgcagtat aagttatact tttaacattg gcagccatat caactgatac
     5221 taaaggcatg agattgagat cgatatcaat atttaatttg tttaaagtgc ctatatctgt
     5281 accagcacgt gctggcgtag cccctgcgag tacataagag actttcatat tattgccaag
     5341 acttttttcg gctgttgtgc caacaatgta gggcataaat tgtttgtcag tcagagtcag
     5401 ttgttgaacc gtccctaaat acggatcaac aaggctggca ctgccatttg tccattcacc
     5461 aaatgataaa gagcctattt gtttaacatt ggtaaattgt agtccaccnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tgttgtacca gcaggtgctt ggttaggggc
     5641 ttggcctgtt tctacctgaa aagtttgtcc tgtattaaca ttactgaagc caaaattatt
     5701 ttcaagattg acaatgccgc tgccaactgt aagtaacaat ttgccattcg gctcttggtt
     5761 ggccgcataa cttgtaccac gaataccaat cgtggcaacg ggggtttgta ccttgtaatt
     5821 agcacggttt actttgccta tcgcaccact cacagtacgc attccacccc gtaaaaaatt
     5881 gaataacaac gaaccatcgt taggtttatt tttatcaaaa ctgtatttat ctaaaccaaa
     5941 taccgtatta ggttgtaatg aaataaaact accatcagta aagcgaattt gcacacgacc
     6001 acgattggtt tctaaacgct cgccactatt gactaattcg ccttttacta aagcgcgacg
     6061 tgaaccgtct gtggcaatca cttcaacatt gcctgataca aaattaactc gacccgcaac
     6121 atcggccaag gcagtacctg aaagagtgta agcaattaat agacttaata agccttttcc
     6181 attgagagaa gtagataaat aattcatggt gagtttcctc cttgttacat atcaacacgc
     6241 aaatcaatgc tgattacatg gcgcgtgaag tcgctaaacg caatgtttga ttggttatca
     6301 ctataattat agctaggaat taacgaaaag tgtttattta ttcgccagtt tgtactgatg
     6361 ttcatactat tttcggtatc accacgataa atatttatta tgggaaaaag ctgtttatta
     6421 tggtatcttt tttcttggct cattcgaata tttaatgaga tatcattggt gatatttaaa
     6481 gagctaccta cagcaattcc tttgaaggag tttgtcaaat attgggtaag attgctttcg
     6541 gcttcttctt gtcctttata tacacttaaa aataaagaag gagtaaatcg tgtctcaata
     6601 tccttataat aaatcatacc cgtattattt cgtttggtat cacgtgtaga taatttgtca
     6661 taattcaatg tgttaatttg tctaaaaata gaggcttgac cgttagcgat ttcagtctgt
     6721 aactgtaata aaccaccttt gatactgcga tattcttctt catttaacca catttgctga
     6781 gcttgtagtt gagttattag ctcaaagctt tctaaatcgg attgtatgct aaagttggca
     6841 tcaattgatt gataatcaaa aatatctgtt gtatagggac tattatcatc gggtaatgat
     6901 ttgtcaaatt tacgagattg gatattgata ttagttaaac tcgtcaaata agggtttatt
     6961 ttgtataaaa gttttgatcc taaatttagg cttatgtagg ctgaatctag tgaggtagca
     7021 gtgttggcga tagtgtttgt acttgatggt gcgctattga tattactatc aaatcccatc
     7081 ccaatttggc tatataaggc taaatgtctt ttttgtgttt tttcgagatt ttgtattgca
     7141 ttcaaataac cacgaacaat gtcgttaatt tcggcaggag gattatattc gttaataatt
     7201 tcaaattcgg cacgagcatt acttgcttca ccaagtgcta aatgggttct agctatttga
     7261 acacgacaag ggccatttgt aggttctact gttaagcaac gctcaaaagc aaaaatagca
     7321 ttttgaggtt tattttgttc gagtagtgac aaaccaagta aataatcaaa ctgaggagtg
     7381 cctgctaatt catcttctaa tggtgcaaga agttcataag cttgttcggg agattggagg
     7441 cgaatcagtg catcggctcg attcaaaata tcttcagaag cccaaaccag tgagttagtt
     7501 gctagtaata gagcaacagc aaaagcattt aagcgcatag ccacagtcca tgagtataaa
     7561 taagcagatt ataagtttag ctaggggggg gatgttgcta tcagtaacac aatttatagg
     7621 caaagcagta atagacccta aactaaatct aaaattttag tatttatacc tgattaatgc
     7681 ttaaatgata agtctttaag tgttttcttt atcagcacgc caatgtttag cccctacaaa
     7741 aattaagcgt aattgcttaa cagcttgcat actcagttca tattgcaaac gtacttgttc
     7801 tgaggggagt gataaaattt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 naataacagg tgttcaccca ttttagcaga atatttttct acaagctaac agttggcagt
     7981 ttagtaaaca agttttggca ggtttgtgta cattacttcg ctgttaaatc aactatatag
     8041 catcaaataa agtaataaat aactttaagc tatcaactgt tatgcaattt atggcaaatt
     8101 tagtgaatta attaactaaa tgttcacatt taaatttatg ttaataaaca attgcttatg
     8161 tttttttgtg ctttttttat gggaaattta aagaaaaata ttaaaaaatt agtgtacaag
     8221 tgttgactta tttttgggtt gggttatatt aagtgcacag atgttcactt atttgcgtag
     8281 gagcgaagaa catgacagca ctgaatcccg ttattggtcg ttttgttggt agcgttaaag
     8341 aagattggct cgattttatg ttggcacata ttgatccgat gctcactgta aaacgtgttt
     8401 tggcaaaagt ggttgctatt actgatgaaa cacaagatat caaacgtttt gttttacaac
     8461 ccaataaaca ttggcagggg tttcaggctg gtcagtttgt cagcgtgcgc atcatgatta
     8521 acggtagctt tcatgagcgt tgttatagct tggttagcgc accaacagac tctgttattg
     8581 aattaggcat taaacgtcag cctaaaggaa aagtatctaa ttggatgcac gataacttaa
     8641 atatcggtga tgtgattgaa ttaggtgctg tgggtggtga ctttaagttg ccagaaacat
     8701 taccaagcaa aatgttgtta attgccggtg gtagtggtgt tacacccatt tatagcttgt
     8761 tagttgaagc gttaaagcgt caaccaaatg ccgatatttc tgtggtttat tatgtaaaca
     8821 cagatagaga tttagccttt ggtaatgaaa tggtggaatt ggcaactaag catcgctctt
     8881 taaaactaca gtatgtttta gctactagtg gtgatgcagg acgttttagc cataaacaat
     8941 tagccgatgt ttgtcctgat tttaccgagc gtcataccta tttatgtggt ccacaaggct
     9001 taatgggtgc agtaaccaaa gtttgggctg aacaaggtat tagcgaaaaa ttaacccaag
     9061 aagtatttgg tttgcctgct gttgatccaa acaagccgat caacgaaatg ccaattacct
     9121 tacgccgtag tcagcaatta ttgttaaaca ctaaaccaac gttattggaa agtgctgaag
     9181 ctggtggtgc aaaacctgct tatggttgcc gaattggcat gtgtaaaacc tgtagttgta
     9241 ccaaagtatc tggtgtagtg agagatgtga ttacaggtgc gattgatgat cagccaaata
     9301 cgcagattcg tatttgtgtg actgaagctt taagccctgt gactttagat atttaagtaa
     9361 gttnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnacaaacc gaattaagat
     9481 taagagagaa ttatcatgtc aacgattagc aaaagccgtg atttaagctt agaagaaatt
     9541 gaagcctttg gtgcagagtt agatgcttta cgcaaagaaa cgatggccag tttaggcaaa
     9601 aaagaagctg actatattta tggtgtgcgt aactttgtgc gttacaccga aattgcaggt
     9661 cgtacttcct taatgttggg ttggttgcct ccgttttggt tggcaggtac agcgattttg
     9721 ggtgcttcta aaattgttga aaatatggaa ttaggccaca acgtgatgca cggtcaatat
     9781 gattggatga atgatccatc attacgtggt aatgactacg aatgggatac cgtatgtacc
     9841 gcagataact ggcgtcatac ccacaacttt atgcatcata cttataccaa cgttcgtggt
     9901 atggatcatg acatcggtta tggcgtattg cgtttgtttc ctgaacaaaa atgggaatca
     9961 cgctttttag caaacccaat tgctgctgct attttagcgg tgacttttga atggacattg
    10021 gctttacaat ctttagagtt agaaaaatta atcagcaaag aaaaaacctt tgctcaagtg
    10081 cgtgaagaat ttaagccaat ttgggcaaaa atgcgtaagc aattcaccaa agattatatt
    10141 ttcttcccgt taattgctgg cccaatgttt attcctgtga ttacagggaa cttttttgcc
    10201 aatatgattc gtaacgtttg ggcattctcg attattttct gtggtcactt tactgaagat
    10261 gccgagactt tcccaatcga agtgttagag aacgaaacac gtggccaatg gtatttacgc
    10321 caattacatg gttctagtaa cttaacaggc aacaaaatta tgcactatat gtctggtaat
    10381 ttaagtcatc aaattgaaca ccatatgttc cctgatgtac ctggtatgcg ttatgccgaa
    10441 atgtcagtca aagtacgtga aatttgtgag cgttatggcc aacattacaa cactggctct
    10501 tttactaagc aattttcgac cgtggtaaaa cgtattttta aatatgcctt accatttagc
    10561 cgtaaagttg aagactcaag agcagaaacg attgtggcta aagcagcaaa agtaacgcca
    10621 atcacccaaa aaactgccac tatcaacagc atagcggcct aagtttttga ctaagctata
    10681 catctactga ctgacaggga ttgttttaat ggattagatt ttgcaataac aaggcttaca
    10741 cttttttaac agaatgaatt ttgtttgtac aatgtgtaag ttcaattttt attgaacata
    10801 gtttctgttt tctggttcag gggctatggg taagtagcgc aaaataaagt cagaatttgt
    10861 ggtgctgtac agataaatag atgtatagtg aagtcaaatt ctaaaaacct cagggcttac
    10921 gcattttatc aaaagcttgt atttaatgaa gtctaaacag gcgcgtagtc cctaacttag
    10981 cggagattta acatgagcaa aaccttgact cgtttagaag ccttaaaaga tttagttcag
    11041 caagcgattg ataccggtgc aacctcggtt gagcaaattc ataaaacgat tgctgcatta
    11101 cctttagctg ttttagaaaa acaaggtttg ttggatgtag atagcgaaaa gcgtgatgaa
    11161 ttatgggata aaagttttgg tcaggtgtac gaagccatac gccgtgttaa tcaagaagtg
    11221 ggcgagttag cgacacaggc ttttgaagcc atagaagatc aaatgattat tcaaaaaaat
    11281 attgctgagg ccgaagcagc agaagcagca aaacaaaaga ttgaatcacc tgtagcggtt
    11341 gaacccaaat ctaaagtagt ttaaagatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11461 nnnnnnnnta aacgttaaac ctgctttagc ctctaaacgc tcaattaaac gttgaccaag
    11521 tgccgtgctg ggtgtccaaa aaccgcctgt tttatcgttt ttgctaatgt ctgtggccaa
    11581 acaaacggcg gcttcaccca acatttttgc ggtagaacca taacctggat cacggtcgcc
    11641 agttactttt gcggttaagc gttgaccatt atcggttaaa ccaatcagac gaatatcata
    11701 aaagccttgt tcttgctgtt taggagtagg gccttcacct gcttggggaa caattttttg
    11761 caaaatacgg cgtgtgggtg gaattactgc cgctgcaaca aaacctgcta aaccaaccga
    11821 tgcgcccacc gccattgccc caccaataac gcctttgcct gtgagcatgg tttcgtcata
    11881 gataaattgt gtgccataag cataattact taaagcatta ctacgatgta ccactttggt
    11941 gttaatccct gccatgacaa acggtgcaga ccaagcttcg gcatctttat catattgcgc
    12001 aaattgtaaa ttgggttgtt tgggtttttg ttggtttgat ggtggacata aagcataagg
    12061 attagccatc actttacgca aactaggatt tttggaaatc tcgctaaaca gttccatcat
    12121 actggcaact gtgccacccg aaaaaccacc acgaatcgct ttaactctaa actttacttg
    12181 ggagcatgtt tgtcccaacc gttgttgagc ttgttgttgc aaataaaacg tgcctaaatc
    12241 ggaaggaata gagtcaaaac cacaacaatg aacaatgcgc gcaccactgg cttgtgcggc
    12301 tgattcgtat tgttcaatca tgcgggcaat ccattgtggt tcgcctgtta aatcacaata
    12361 atctgtgcct aaggttgcac acactttaac taagctagag ccatacaagg cataaggacc
    12421 aaccgttgat accacaactt tagtttgttg acacatggct ttaagggcag attcgtcttg
    12481 agcatcggca ataattgtcg ctaattggct agctcctgag cctaaatttt gttttaattc
    12541 atttagttta cttgccgaac gtcctgcaat tgcccatttt aattgttgat taacaccata
    12601 agtttgcact aaataacgag ttaataattg gccaacaaaa ctggttgcac cataaacgac
    12661 taattgatat tgcgacatag ataaaaccct tgggataaat ttaacaaggg ttgtagccta
    12721 atttacgcca ataaacaagt tatacaaatt tgagaggaga gataaataat gactaattcg
    12781 accaaggctg atttgttgtt ggtgatagtg acattattag ccgcagtcag ttggattatg
    12841 tctaaaga
//
LOCUS       DAOQMB010000024        12739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1867_length_12739_cov_15.188663, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000024 DAOQMB010000000
VERSION     DAOQMB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12739
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1867_length_12739_cov_15.188663"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..761
                     /locus_tag="PLY05_01935"
     CDS             105..761
                     /locus_tag="PLY05_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02091.1"
                     /translation="MQNIPQENTESSQQDDGLIEFIAPDIEHLTIIKASETSQIERPK
                     IVFSDELIPQFQLGHTDIRIELKHLFMMQQAAIALLAEAQREILIVSPDLEHERFDNE
                     AFCEALSAFARSSRYTSTKILIADPQLAIEDGHRVIKLMRRISSLIEIRQLHEHDIEH
                     AEAVMIADNIGLLRCINRDPWQGSLLPKGTPYAQQMRERFMEWWERGAEISDFRELKI
                     "
     gene            complement(771..1223)
                     /gene="gspM"
                     /locus_tag="PLY05_01940"
     CDS             complement(771..1223)
                     /gene="gspM"
                     /locus_tag="PLY05_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNC02092.1"
                     /translation="MPAVIEQYLAKLSPRDRIAVLVLTLFIVVSLCGLMALKLHRAAE
                     KAQQQAMQEKELYTWFQANLPVLSHGSSSNNGMSVLDTVSASAGGMGINMNRFEPDGE
                     QVRVWLENTDFAKVASWLHTLSQQGVKAQEVHFEQNNKGLSVRLVFGR"
     gene            complement(1225..2415)
                     /gene="gspL"
                     /locus_tag="PLY05_01945"
     CDS             complement(1225..2415)
                     /gene="gspL"
                     /locus_tag="PLY05_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HNC02093.1"
                     /translation="MDTLYIRIPTINGLWQGWLLANNSTQAKVLSQHELEAIMAAKAA
                     NMRIHLLLPTSACVIAPVNITKQQLKQLSENDIAYLLEDQVLTSVEQLHCVYQSLDEQ
                     QALVLAIQQNVLDDLLQPFKDIQGELLSAVPDIFLLPVNPQGWSLLVDNTDCWLRLNQ
                     TWGVRLEANAAVALLDSAWQEHPTSHIQVYGEVSTDVQAWLKLQDNLTIDVLPELDWT
                     NQFSQIHNKHPFNMLKGDFAVKTKSSLSGYWRYAAIFVGIAFATQVGYDSARLWHYNK
                     MIKQTKAESTKLYKSLFPEEQRIVNLRRQIESHLAERQNAGQGFMPIATRVGEVLNTG
                     NWQTQRMDFDNNGLLLEVDAMSLSDLEQLRQQLSGQGLNTETLSANSQGTGIRGRLRI
                     TENS"
     gene            complement(2418..3416)
                     /gene="gspK"
                     /locus_tag="PLY05_01950"
     CDS             complement(2418..3416)
                     /gene="gspK"
                     /locus_tag="PLY05_01950"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HNC02094.1"
                     /translation="MPHNKQQGVALITVLLVVAMAMVLAVAMVKSQQSLLRRSSSVFS
                     QDQAYLYTLGAESFAKSILQDDKDKDKNKNTPQDSLNENWAKKIPAFPVEGGFVQAQI
                     EDLQGKFNLNTIWQEGQIDMEAVTTYQRLLVALDISPMLVSPIIDWLDTDNLPYDSDG
                     AEEDWYLRLKPAYRAANHPFVSIEELALLRGYTPEIVAKLKPYVSALPAKTTININTA
                     SDVVLAALSDNLTLNMAKDMVSRRPKEGYGSVDNFMQLSEFANLSQEDKQKISKLIGV
                     SSGFFSVLAESEIDNRRRILQATVAFDESSNAQTIRRDWSQQWRPQVQISSSTTSK"
     gene            complement(3403..4032)
                     /gene="gspJ"
                     /locus_tag="PLY05_01955"
     CDS             complement(3403..4032)
                     /gene="gspJ"
                     /locus_tag="PLY05_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HNC02095.1"
                     /translation="MKQRGFTLLELLVAVAIFALLGVGSYRLLASTISTRDAARTHDL
                     ALMQLQRTFTVINRDLAQATARPIRDQYGDTVPALQLKDNNLELTRAGVPNPLKQVRS
                     DLQRVSYQLNNKGELIRSSWQQLDRDRGVNPQQTILLTKIKTMTLKAYNPTGSASPEW
                     PVAQSYNKPPVDTLPRSVEIVVNVEPWGEVRRIFALPQQLEKEQKSATQ"
     gene            complement(4029..4430)
                     /gene="gspI"
                     /locus_tag="PLY05_01960"
     CDS             complement(4029..4430)
                     /gene="gspI"
                     /locus_tag="PLY05_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HNC02096.1"
                     /translation="MKNKQRAFTLLEVLVALAVFSVAAISLMKVSESQLRLSQRMEEK
                     TFAHWVALNMITEMQGSQDWPNIGEQTGKVSMAGREWKMVVKTQATPVSRVRRIEITV
                     GLAPKDFTDDMENLTMLTGFIEQPSGMGGEP"
     gene            complement(4427..4975)
                     /gene="gspH"
                     /locus_tag="PLY05_01965"
     CDS             complement(4427..4975)
                     /gene="gspH"
                     /locus_tag="PLY05_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HNC02097.1"
                     /translation="MIARQQQGFTLIEVLLVVLLIGIVSGIVLLAASPNDSSRVVATE
                     TERLAEVLVLASEEAINNNQQMGLLFDERSYRLMVLDETSQRWQESNEPLFAAYELPE
                     IVNLHLLKDDKDKLIVLDKDKNNKQDDTQAITPQLLFLSSGESSSVVLEIASEDGNKQ
                     QISVDDLSSVTLGSQAPVEQSR"
     gene            complement(4981..5397)
                     /gene="gspG"
                     /locus_tag="PLY05_01970"
     CDS             complement(4981..5397)
                     /gene="gspG"
                     /locus_tag="PLY05_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNC02098.1"
                     /translation="MKKSQQSGFTLIEVMVVVVILGILAAVIVPNVIGKDEQARRTLA
                     TSSLSNVANALEMYRLDNHKYPTTQEGLEALVTKPASAKVFPAGGYLKSIPNDPWDNP
                     IQYVSPGANGKPFELYSFASDGQEGGEGDNADIYYK"
     gene            complement(5449..6669)
                     /gene="gspF"
                     /locus_tag="PLY05_01975"
     CDS             complement(5449..6669)
                     /gene="gspF"
                     /locus_tag="PLY05_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005301929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HNC02099.1"
                     /translation="MPAFDYIALDAQGKKQKGVLEGDSARQIRQQLRDKGWFPTSVEA
                     ASSEQANNEKTPFWQSSKGLTAYELALVTRQLATLIQAGIPLEETLKAVAKQSEKPQV
                     QSLLLAVRGKVLEGYPLAQSFAAFPKAFPDLYRATVAAGEKSGHLDLVLQQLADYTEN
                     RFTTQKQIQGALIYPIILTTLAIAIVVGLLTYVVPDIVKVFTNSHQQLPFLTRALIAA
                     SNGVKTFAPLIVLVLVVGAWLFKKSLDTEKGRYAFDGFVLRVPLVRRISKGANAARFA
                     STLSILSKSGVPLVEALYIAAAVASNWHIRDAILEAAVKVTEGGSISGSLEKSRYFPP
                     MMIQMLRSGETGGELDEMLARSAQMQDRELSSLITTMVGLFEPLMLLVMAGVVLIIVL
                     AIMLPIVSMNSLVH"
     gene            complement(6672..8150)
                     /gene="gspE"
                     /locus_tag="PLY05_01980"
     CDS             complement(6672..8150)
                     /gene="gspE"
                     /locus_tag="PLY05_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNC02100.1"
                     /translation="MSRQGQLAYSFAKRHGVLLVKQGETQEQGLLLREDVKLASLMEV
                     RRVLGFLIVHETLATADFNQRLATAYQGDGGESQQAVEGLEDHPDLESLAESVPETED
                     LMEQEDDAPIIRLINALLSEAIRLSASDIHIEAFEKRLSVRMRVDGMLREIVQPRREL
                     APLLVSRIKVMAKLDIAEKRVPQDGRISLRLAGREVDVRVSTLPANHGERVVMRLLDK
                     QAGRLDIGHLGLQATDHKRLRDLIHKPHGIILVTGPTGSGKTTTLYASLTELNDNSRN
                     ILTVEDPIEYQLEGIGQTQVNTKVDMTFARGLRAMLRQDPDVVMVGEIRDKETAEIAV
                     QASLTGHLVLSTLHTNTAVGAVTRLQDMGIEPFLLASSLLGVVAQRLVRVLCASCRQG
                     VTADEEECALLGVSPQFAPTIYRPVGCEKCNFNGYRGRLGIYELVVIDDNMRRLIHSH
                     AGEHELEAHARTMSSSIRADGIRKVLKGITTLEEVVRATREG"
     assembly_gap    8166..8265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8397..9188
                     /locus_tag="PLY05_01985"
     CDS             8397..9188
                     /locus_tag="PLY05_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02101.1"
                     /translation="MSNTIWWGNHQLAIDEQRRWQMAGLLLHIQRDAQEWQIAYHRSP
                     NQHEDDHDWQIIEHTDRSPMPEKALLQRYIYNQTQQQIHLKPCLADRSVVIKPITPLF
                     LSSNQQTTLFVSTPLWLNIFSDDDNLLLDIPFVRPTDTWFGATPIRGEICYATKVFGR
                     LALEQLPIRAFRAVTPIHIVNHHTKAIQIERINIPAPLLPLYAAQDGRLWTPTIEVEQ
                     LANGRTPKVSISKHLHPKAGNVKLLNQARQHDDNNLINLFEHFFE"
     gene            9196..10050
                     /locus_tag="PLY05_01990"
     CDS             9196..10050
                     /locus_tag="PLY05_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005182510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNC02102.1"
                     /translation="MASNKQESFLDQMINLPVSDKIEGFIIGALVLLTGYLIAQLVTT
                     AFVKTMSQRLSAHQLLVWRRGIFYFLLGLFSMSALHEMGFKLSVLLGAAGIFTVAIGF
                     ASQTSASNLISGLFLIGEGSFMVGDTIEVNGTIGEVLSIDLLSIKLRTADNIYVRIPN
                     EQLIKSEVSNLTRFAIRRVPIMVNISYQQDIGKVRQVLLTIADKNPLVLDEPKPQVVV
                     QDLASSSINLMFNIWTRKENFLDIRDTIQESIKNAFDEHNIDIPFPQITVNSGVIAPL
                     VVSMKNQA"
     gene            10478..11089
                     /locus_tag="PLY05_01995"
     CDS             10478..11089
                     /locus_tag="PLY05_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02103.1"
                     /translation="MSQKDQAIVQLEGQLASLSRRRFLKAGLILGTSAATVITYPTRV
                     FADTGVPTYIRHLSEAEYRLFDKLRQVFLPTERFPDLPSTTEVPVMENLDNMVGRLNS
                     DTRFLLSIGAKTLEFSTLYKMKRFSSLNNKQARNQILAWQSGISIQSGLIVSFKTLLG
                     VAYWRDPRTWQPLEYDGPVTAKWGIRRLGNMPVPRDQDDNAKI"
     gene            11102..12694
                     /locus_tag="PLY05_02000"
     CDS             11102..12694
                     /locus_tag="PLY05_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNC02104.1"
                     /translation="MIYKELPADGIPVTQASDIRSSDDIILNADVVVVGSGTGGAVIA
                     HELAVAGKSVIILEAGRYVPSSQFTEDMAWALENLYQDVGTQTNTIGDTVVLQGRCVG
                     GSSVVSATIAARAPDYVLESWVKDHGIEGLAPNKINRYYEKVENRLHVHINEPHEIND
                     CANAIIRGCEALGWSWRPQSRNVKQCALTGHCLAGCPSDRKASMQVTYLPWAIAAGAK
                     LYADTYVETILMKNGRAVGVSGRIIDPNNHSVIANIKVNAQIVVAAAGAIHTPLLLQR
                     SKVPNKNDQLGRNLFLQPTSVVLGKFPVPVYGWRGAFAGMHIDEFLNESGDKLLMESG
                     LAAPVQLMAQNELSLGIDHIRFMQEFKYFSALNVLMPDTGHGFVHWSGPMRGGSKRIE
                     WNMTKDEFEIYKRGLKRAGRIFFAAGAERVYLPTYQRIQATSVNELDDIVDSIDYGTL
                     GLFSTRLVSLNAQGSCRMGADRKKAVVDQYGQVYEVSGLFVSDASLLPSLAVQHPMLT
                     VAALSHYIADQILTRHQGYFWS"
BASE COUNT         3815 a         2690 c         2266 g         3868 t
ORIGIN      
        1 ctgtcaatct cagtccaata atgtattaat cgaatgtata tatcaggatt tagccatttc
       61 acctgctata atttaaacaa taaacagcct caagtgttcg agttatgcaa aacataccac
      121 aagaaaacac cgaatccagc caacaagacg atggattgat cgagtttatt gctccagaca
      181 tagagcattt aaccattatt aaagcgagtg aaacaagcca aattgagcgg ccaaaaattg
      241 tctttagtga cgagctaatt ccacaatttc aattaggcca taccgatatt cgtatagaac
      301 taaaacatct ttttatgatg caacaggctg cgattgcatt attagctgaa gctcaacgtg
      361 agattttgat tgtcagtcct gatttagaac acgaacgctt tgataatgag gcattttgtg
      421 aagcgttatc agcctttgca cgttctagtc gttatacaag tacaaaaatt ttaatcgctg
      481 acccacaatt agctattgag gatggccatc gtgtgattaa attaatgcgg cgcatttcca
      541 gtttaatcga aatacgtcaa ttgcatgaac acgatataga gcatgcagaa gctgtgatga
      601 ttgctgacaa tataggttta ttacgctgca ttaatcgtga tccttggcaa ggatctctac
      661 tgcctaaagg cactccttat gcccaacaaa tgcgtgaacg ttttatggaa tggtgggagc
      721 gaggcgcaga aattagtgat tttagagaat taaaaattta gtctctaaac ttaacgtcca
      781 aagactaaac gcacactcaa acccttatta ttttgttcaa aatgaacttc ttgcgctttt
      841 acaccttgtt ggctaagcgt atgtaaccag cttgctactt tagcaaaatc ggtattttct
      901 aaccaaacac gcacttgttc accatctggc tcaaaacggt tcatattaat ccccattcca
      961 cctgcactcg cactaaccgt atctaaaaca gacataccat tatttgagct actaccatga
     1021 cttaatacag gtaaattggc ttgaaaccaa gtgtataatt ctttttcttg catcgcttgt
     1081 tgttgagctt tttcagcagc acgatgtaat ttgagtgcca ttaatccaca taaagacacg
     1141 acaataaaca aagtcaacac aagaacagcg atacgatctc taggacttaa ttttgctaaa
     1201 tattgttcaa tgactgctgg cattttagct attctcggta atacgtaaac gaccacgaat
     1261 tcctgtgcct tggctattgg cacttaaggt ttcagtattt aaaccttgtc cacttaattg
     1321 ctgacgcaat tgttccaaat cactcaaact cattgcatca acttctaata ataagccgtt
     1381 attatcaaag tccatccgtt gtgtttgcca attaccagta tttaatacct cgcctacacg
     1441 tgtcgcaata ggcataaatc cctgacctgc attttgtcgt tctgccaagt gtgattcaat
     1501 ttgtcggcgt aaattaacaa tacgttgctc ttcaggaaac agtgatttgt ataactttgt
     1561 gctttctgct ttagtttgct taatcatttt attgtaatgc cataatctag cactgtcata
     1621 cccaacctgt gtagcaaagg caatacccac aaaaatcgca gcataacgcc aataacctga
     1681 taaagaagat tttgttttta ccgcaaaatc acctttaagc atattaaaag gatgtttatt
     1741 gtgaatttga gaaaactgat ttgtccaatc taactctggc aaaacatcta tggtcaagtt
     1801 atcttgcagt tttaaccatg cttgaacatc tgttgacact tcaccataaa cttgtatgtg
     1861 agatgttgga tgttcttgcc atgcactatc taataacgca acagcagcat tcgcctccaa
     1921 acgtacaccc catgtttgat ttaaacgcaa ccaacaatcg gtattatcca ccaataatga
     1981 ccaaccttga ggattaacag gtaataaaaa gatatcaggg actgcactta acaactcgcc
     2041 ttgaatatct ttaaaaggtt gtaataaatc atctaaaaca ttctgttgaa tcgccaatac
     2101 taaggcttgc tgctcatcta aactttgata gacacaatgc aactgctcta cagaggttaa
     2161 tacttgatct tctaataaat aagcaatgtc attttcgctt aattgtttaa gttgttgttt
     2221 ggtgatatta acaggcgcaa tgacacaagc cgatgttggc aacaacaagt gaatgcgcat
     2281 attagctgct tttgcagcca taatcgcctc aagctcgtgt tggcttaata cttttgcttg
     2341 tgtgctgtta ttggctaaca accaaccttg ccacaaacca ttgattgtcg gaatacgaat
     2401 ataaagagta tccattgtta cttacttgtt gttgaagacg aaatttgtac ttgcggacgc
     2461 cattgctggc tccaatctcg gcgaatagtt tgtgcattac ttgactcatc aaaagcaacc
     2521 gttgcttgca aaatccgacg acgattatca atttctgact cagctaaaac actaaaaaac
     2581 cccgagctta ccccaattaa tttactaatt ttttgtttat cttcttgaga aagatttgca
     2641 aactctgaca actgcataaa attatctaca gagccatatc cttctttagg tcgccgacta
     2701 accatatctt tggccatatt cagcgttaaa ttatcactta aagcggccaa aaccacatca
     2761 gatgctgtat taatatttat cgttgttttg gcaggtaacg cgctcacata cggtttaagt
     2821 ttagccacaa tttctggtgt ataaccgcgc aataaggcta actcttctat cgacacaaac
     2881 ggatgatttg ccgcacgata ggcaggcttt aggcgcaaat accaatcttc ttctgcacca
     2941 tcactatcat aaggtaagtt gtcggtatcc agccagtcta taatgggtga cactaacata
     3001 ggcgatatat caagagcaac caataaacgt tgatatgtgg tcacagcctc catatctatt
     3061 tgcccctctt gccaaatcgt attcaaatta aatttgcctt gtaaatcttc aatttgcgcc
     3121 tgcacaaaac caccttctac gggaaaagca ggaattttct tcgcccaatt ttcatttaaa
     3181 ctatcttgag gtgtattttt gtttttatct ttatctttat cgtcttgcaa aatagatttg
     3241 gcaaacgact ctgcccctaa agtatataaa taagcctgat cttgactaaa cacactactg
     3301 gaacgacgca ataaactctg ttgactcttt accattgcaa cagccaacac catagccatt
     3361 gcaaccacca ataatacagt aattaaggca acgccttgtt gtttattgtg tggcactttt
     3421 ttgctctttt tctaattgtt gtggcaaagc aaaaatacgt ctaacctctc cccaaggctc
     3481 aacgtttacg acgatctcaa cactgcgtgg taaggtatcg actggtggct tattataact
     3541 ttgtgcaact ggccattcag gactagcact acccgttgga ttatatgctt ttaatgtcat
     3601 tgtttttatt ttagttaata gaatagtttg ttgcggatta acaccacgat ctcggtctaa
     3661 ttgttgccaa gaagaacgta ttaattcacc tttattatta agttgataac tgactctttg
     3721 caaatcactg cggacttgtt taagcggatt aggcacacct gcacgagtta actctaaatt
     3781 attatctttt aattgtaatg caggcacagt atcgccatat tgatcccgaa tgggtcgagc
     3841 cgtcgcttgt gctaaatctc gattaattac cgtaaatgta cgttgtaact gcattaatgc
     3901 caaatcgtgt gtgcgtgcag catctcttgt tgaaatggta cttgccaaca aacgataact
     3961 acccacacct aataaggcaa aaatagcgac tgcaactaat aattctaata aagtaaaacc
     4021 tcgttgtttc acggctcccc tcccatacca gaaggttgct caataaaacc tgttaacatg
     4081 gttaaatttt ccatgtcatc agtaaaatct ttgggtgcta aaccaacagt aatctcaata
     4141 cgtcgtacac gactaacagg tgtcgcttgc gtttttacta ccattttcca ctcgcgtcct
     4201 gccatactca ctttgcctgt ttgctcgcca atatttggcc aatcttgact accctgcatt
     4261 tcagtaatca tatttaaagc aacccaatgg gcaaaggttt tttcttccat acgttggctt
     4321 aaacgtaatt gtgattcact cactttcatt aaagaaatag ccgccaccga aaacacggcc
     4381 aaagccacta atacttctaa caatgtaaaa gcacgttgtt tatttttcat cgactttgct
     4441 caacaggtgc ttgactaccc aacgtgacag agcttaaatc gtcaacagag atttgttgtt
     4501 tattaccatc ctcactggct atttctaata caaccgaaga actttcacca ctggataaaa
     4561 acaataattg tggtgtaatc gcctgcgtat catcttgctt attattttta tctttatcta
     4621 acacgatgag cttgtcttta tcgtctttta ataaatgcaa attaacaatt tctggtagtt
     4681 cataagctgc aaataaaggc tcattacttt cctgccaacg ttgactagtt tcatccaaga
     4741 ccatcaatcg atagctacgc tcatcaaata acaaacccat ttgttgatta ttattaatcg
     4801 cttcttcact tgctaagacc aaaacttcag ccaaacgctc agtttcggta gcaacaactc
     4861 gcgaagaatc attagggcta gccgccaaca agacaatacc actgacaata ccaatcagta
     4921 gtaccactaa aagcacttct attaaggtaa agccttgttg ttgacgtgct atcatcttca
     4981 ttatttgtaa taaatatcag cgttgtctcc ttcgccacct tcttgtccat cgctggcaaa
     5041 cgaatacaat tcaaaaggtt tgccgttcgc acccggtgaa acatactgaa taggattatc
     5101 ccaagggtca ttaggaatac tttttaaata tcctcctgct ggaaaaactt tggcagaagc
     5161 aggcttggta actaaggctt ctaaaccttc ttgagtcgtt gggtatttgt gattatctaa
     5221 acgatacatt tcaagtgcat tagctacatt agacaaacta ctcgttgcta aggtacgacg
     5281 tgcttgttca tctttaccaa tcacattcgg cacaatcaca gccgctaaaa tacccaaaat
     5341 aacaacaaca accatcactt caattaaagt aaagccactt tgttgggact tcttcatgtc
     5401 atttctccat caaaatactt aaattaatat cttgatttat aaatattatt aatgcactaa
     5461 actattcata ctaacgattg gcaacatgat tgccaagaca ataattaaca ccacacccgc
     5521 cattactaat agcattaatg gttcaaacaa accaaccatt gtcgtaatta acgatgataa
     5581 ctctcggtct tgcatttgtg cagaacgagc caacatttca tctaattcac cacccgtttc
     5641 accacttctg agcatttgaa tcatcatcgg tggaaaatag cgactttttt ctaaagagcc
     5701 actaatacta ccgccctcag tgactttcac cgcagcctct aaaatggcat cacgaatgtg
     5761 ccaattgcta gcaacagccg cagcaatata caaagcttca accaaaggca caccactctt
     5821 actcaaaata cttaaagtac tggcaaaacg cgcagcattt gcacctttag aaatgcgtcg
     5881 cactaaagga actcgcaaga caaatccgtc aaaagcataa cgaccttttt cggtatctag
     5941 cgatttttta aacaaccaag caccaactac taagaccaac acaattaatg gggcaaaggt
     6001 ttttacgcca ttactggctg caattaaagc acgggttaaa aacggcagtt gttgatgaga
     6061 gttggtaaat actttaacaa tatcaggcac aacataggtt aataaaccaa ccacaatcgc
     6121 aattgctaaa gtcgttaaaa taataggata aattaatgcc ccttgaattt gcttttgtgt
     6181 tgtaaaacgg ttttcggtgt aatcggcaag ctgttgtaat accaagtcta aatggccaga
     6241 tttttctcct gccgcaaccg ttgcacggta taagtcggga aaggctttag gaaatgcagc
     6301 aaaactttgt gctaaaggat aaccctctaa cactttgcca cgcacagcta ataacaaact
     6361 ttgtacttgt ggtttttcac tttgtttggc cacggctttt aaagtttctt ctaaaggaat
     6421 acccgcctga attaaggtcg ctaactgacg tgtgactaaa gctaattcat aagcagtcaa
     6481 tcctttactg gattgccaaa aaggtgtttt ttcgttgttt gcttgttctg aagatgctgc
     6541 ttcaacagag gttggaaacc aacccttatc gcgtaattgt tgacgaatct gccttgcgct
     6601 atcgccttca agtacacctt tctgtttttt gccttgtgca tcaagggcaa tataatcaaa
     6661 tgctggcata attacccttc acgcgtggct ctgaccactt cttctaatgt tgtaatgcct
     6721 tttagcacct tacgaatacc atctgcacgg atactactac tcatagtacg cgcatgagct
     6781 tctagttcgt gttcccccgc atgactatga attaaacggc gcatgttgtc atcaataaca
     6841 actaattcat aaatgcctaa acgaccacga taaccattaa agttacattt ttcacaacca
     6901 acaggccgat aaattgttgg tgcaaattgt ggtgaaacac ctaataaagc acattcctct
     6961 tcatcagcag tgactccttg acgacagcta gcacataaca cgcgcactag acgttgagca
     7021 accaccccta ataacgaaga agccaacaaa aatggctcaa tgcccatatc ttgcaaacgg
     7081 gtcactgcac ctacggcggt attggtgtgt aaggttgata atactaagtg acctgttaat
     7141 gacgcttgga cagcgatttc ggcggtttct ttatcacgaa tctcaccaac catcaccaca
     7201 tcagggtctt gacgcaacat agcacgcaaa ccacgagcaa aagtcatatc aactttggtg
     7261 tttacttgag tttggccaat accttctaat tgatattcga tagggtcttc aacagttaaa
     7321 atattacgag aattatcatt gagttcggtt aatgaggcat ataaggttgt ggttttacct
     7381 gaacctgttg ggccagtgac caaaataatg ccatgaggct tatgaattaa atcacgtaaa
     7441 cgtttgtggt cagtggcttg taagcccaaa tgaccaatat ccaaacgccc tgcttgttta
     7501 tctaacaaac gcatcaccac acgttcacca tgattagcag gcaaggttga tacacgaaca
     7561 tcgacttccc gacctgctaa acgcagagag atacgaccat cttgtggcac acgcttttcg
     7621 gcaatatcca atttagccat gaccttaata cgagacacta ataagggcgc taattcacga
     7681 cgaggctgaa caatttctcg caacatacca tcaacacgca ttcgcactga taagcgtttt
     7741 tcaaaggctt caatatgaat atcagaggca ctcaggcgaa tggcttcact taataaggca
     7801 ttaattaagc gaataatggg cgcgtcatcc tcttgctcca tcaaatcttc tgtttctggc
     7861 actgattcag ctaaactttc taaatcggga tgatcttcca aaccttcaac ggcttgttgc
     7921 gactcaccac catcgccttg atatgctgtg gctaaacgtt gattaaaatc tgccgtggct
     7981 aaagtttcat gaacaatcaa aaagcctaat acacgcctga cttccatcaa gctcgctagc
     8041 ttgacatctt cgcgtaataa caaaccttgc tcttgtgtct caccttgttt aacaagtaac
     8101 acaccatgac gttttgcaaa actataagct agttgaccct gacgggacat aatgtttctc
     8161 ttcttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnatatt ttgaagtcta
     8281 atagatgaca agagcggact caatctactc tgagtagatt tttacaagtc tacgaaaaaa
     8341 gattctgttt tttcgccatt aattgcatat attttataca aacatttgag gtaagcatga
     8401 gcaacacgat ttggtgggga aatcatcaat tagcaataga tgaacaacga cgttggcaaa
     8461 tggctggact cttactgcac attcaacgtg atgctcaaga atggcaaatt gcatatcatc
     8521 gttctcccaa tcaacacgaa gacgatcatg attggcaaat tattgagcat actgatcgta
     8581 gccctatgcc cgaaaaagca ttattacaac gttatatcta taatcaaaca caacaacaaa
     8641 ttcatctaaa accctgttta gccgatcgct cagtggttat taaaccaatc actccgctat
     8701 ttttatcctc caaccaacaa accacattat ttgttagcac acctttgtgg ctcaatattt
     8761 tttcggatga cgataatttg ttgttagata taccttttgt ccgtcccacc gatacatggt
     8821 ttggtgcaac acccattcga ggggaaattt gttatgccac caaagttttt ggtcgtttag
     8881 ctttagagca actgccgatt cgtgcgttta gagcagtcac gcccattcat attgttaatc
     8941 atcatactaa agccattcaa attgaacgga ttaatattcc tgccccttta ttacccttat
     9001 atgctgctca agatggccgt ttatggacac caaccatcga agttgaacaa ttagccaatg
     9061 gtcgcacacc taaagtctct attagcaagc acttacaccc taaagcgggt aatgtgaagt
     9121 tgttaaacca agcacgtcag catgacgata ataatctgat aaacttgttt gaacattttt
     9181 ttgaataatt gaattatggc cagtaacaaa caagaaagct ttttagatca aatgattaac
     9241 ctacctgtga gtgacaaaat tgaaggtttt attattggtg cattggtact attaacaggc
     9301 tatctaattg ctcaattagt cactacagct tttgttaaaa ccatgagtca acgactatcc
     9361 gcacatcaat tattagtttg gcgacggggt attttttatt ttctactcgg tttgtttagt
     9421 atgtctgccc tacatgaaat gggttttaaa ctcagtgtct tgttaggtgc agcaggtata
     9481 tttaccgttg ccattggttt tgcttcgcaa acttctgctt ctaacttaat cagtggttta
     9541 tttttgattg gtgaaggctc atttatggtc ggtgacacca ttgaagtaaa cggtacaatt
     9601 ggtgaagtat tatctattga tttattgtcc atcaaactgc gtactgctga taatatttat
     9661 gtacgtattc ctaatgaaca actcattaaa tccgaagtgt ctaatttgac tcgttttgcc
     9721 attcgccgtg tgccaattat ggtcaacatc agctatcaac aagacattgg caaagtacga
     9781 caagtattat taaccattgc cgacaaaaat cccttagtgt tagatgaacc taaaccacaa
     9841 gttgttgttc aagaccttgc tagttcgtct atcaatttaa tgtttaatat ttggacacgt
     9901 aaagaaaact ttttagatat tagagacacc attcaagaaa gcatcaaaaa tgcctttgat
     9961 gaacataata ttgatattcc ctttcctcaa attaccgtaa atagtggtgt tatcgcccct
    10021 ttggttgtaa gtatgaaaaa ccaagcttaa ctttgcccaa tttagtccta gagattaaat
    10081 ttgagccatt ttattaacaa gaaaaacaat tatcctataa acatgggctt aaatagttga
    10141 gctgtttaag ctagactatt agccaattta tgtggttaac atcaacttgt ttattaaaca
    10201 atcactccaa agatttttag taaacaaaac atgctaatct cgatgcaatg cgtaaatcct
    10261 atcttgcatt aaaaataact cgggtttacg cttgtagatt aaatttttag gctcaaaaat
    10321 cgccaaaaac gtgtaaatag ataataacaa gacttattgg aaatcaataa gtcaaagata
    10381 aaaacaagac ttagctttta cataacacat tatgttttat gacatcagcc aacacaaaac
    10441 taagtcttac aagaaaaatt atcacttggg gatgcgtatg tcacaaaaag accaagccat
    10501 tgttcaactc gaaggtcaac tggccagtct atcacgtcgt cgttttttaa aagctggatt
    10561 aattttaggc actagtgctg cgactgtaat cacttatccg acacgtgttt ttgctgatac
    10621 tggtgtacca acttatattc gtcatttgtc tgaggcagaa tatcgcttat ttgacaaatt
    10681 acgccaagta tttttgccta cagagcgttt tccagatttg ccatccacga ctgaagtgcc
    10741 tgtcatggaa aacctcgata atatggtcgg tcgcctcaac agtgacaccc gctttttatt
    10801 atctattggt gctaaaacct tagagtttag tacgctctat aaaatgaaac gtttttctag
    10861 cttaaacaat aaacaagctc gcaatcaaat tttggcttgg caaagtggta tatcgattca
    10921 aagtggtttg attgtgagtt ttaaaacact gttaggtgtc gcttattggc gtgatccacg
    10981 tacatggcaa cctttagagt atgatgggcc agtaaccgct aaatggggta ttcgccgttt
    11041 aggtaatatg cctgtaccac gtgaccaaga cgataacgct aaaatctaag gataacgcaa
    11101 gatgatttat aaagaacttc ctgccgatgg tattcccgtt acccaagcaa gcgatatccg
    11161 tagtagtgat gacatcatct taaatgctga cgttgtggtg gttggctctg gtacaggtgg
    11221 tgctgttatt gcccatgaac ttgcagttgc gggtaaaagt gtcattattt tagaagcagg
    11281 ccgttatgta cccagtagcc aatttactga agatatggct tgggcactcg aaaatctcta
    11341 tcaagacgtt ggcacacaaa caaatacgat tggtgatact gtagttttac aaggccgttg
    11401 tgtgggtggc tcttctgtcg ttagtgctac aattgctgcg cgcgctcctg attatgtttt
    11461 agaatcttgg gtaaaagatc atggtattga gggtctagct cctaacaaga ttaatcgtta
    11521 ttacgaaaag gtcgaaaatc gccttcatgt ccatattaac gaacctcatg aaattaatga
    11581 ctgtgctaac gcgattattc gtggctgtga agcattaggt tggtcttggc gacctcaatc
    11641 tcgtaacgtc aaacaatgtg ctttaacagg tcattgttta gctggctgcc catctgaccg
    11701 caaagcctct atgcaagtaa cctatttgcc gtgggcaatt gccgcaggtg ctaagcttta
    11761 tgcagatact tatgtcgaaa cgattttaat gaaaaatggc cgtgcggttg gtgtttctgg
    11821 tcgaattatt gatccaaata accactctgt cattgctaac atcaaagtaa atgcgcaaat
    11881 tgtcgttgct gctgcgggtg caattcatac cccactctta ttacaacgta gcaaagtgcc
    11941 aaataaaaac gatcaattag gtcggaactt atttttacaa ccaacaagtg tggttttagg
    12001 aaagtttcct gtgcctgtat atggttggcg tggtgcattt gcggggatgc acattgacga
    12061 atttttgaat gaatctggtg ataaattatt aatggagtcg ggcttggctg caccagttca
    12121 gcttatggca caaaatgaat tatctttggg tatcgaccat attcgtttta tgcaagagtt
    12181 taaatatttt tctgcactta acgtgttaat gcctgatacg ggtcatggtt ttgtccattg
    12241 gagtggccca atgcgtggtg gctccaaacg tattgaatgg aacatgacta aagacgaatt
    12301 tgaaatctat aaacgtggtc taaaacgtgc tggccgaatc ttttttgcag caggtgcaga
    12361 acgtgtgtac ttaccgactt accaacgtat tcaagccact tctgtcaacg agcttgatga
    12421 cattgttgat agtattgatt atggtacttt aggattattt tctactcgtc ttgtttcctt
    12481 gaatgctcaa ggaagctgcc gtatgggagc tgatcgcaaa aaagcagttg ttgaccaata
    12541 tggccaagtt tatgaggtga gcggtttatt tgtcagtgat gccagtttat tacctagttt
    12601 agctgtacaa catccaatgt taacggtggc tgcgttatcg cactatattg ccgatcaaat
    12661 tttaactcgt catcagggct atttttggag ttaaagctca aaggtgtctt tttattcatg
    12721 tttaaaagcc tcatttgag
//
LOCUS       DAOQMB010000025        12651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1889_length_12651_cov_16.769292, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000025 DAOQMB010000000
VERSION     DAOQMB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12651
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1889_length_12651_cov_16.769292"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..2397)
                     /gene="ptsP"
                     /locus_tag="PLY05_02005"
     CDS             complement(91..2397)
                     /gene="ptsP"
                     /locus_tag="PLY05_02005"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004876644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNC02105.1"
                     /translation="MIMLDILRKLVQDVDNSPTLLAALDVIVQSVRDALHTEVCSIYL
                     LDERNSRYVLMASHGLNPDAVGNVSLGLSEGLVGLVGQREELINLEDAASHPRYHYLP
                     ETGEERYSSFLGVPIMNRRKVLGVLVVQQQDKRRFGQAEEAFLVTLSAQISGVVAHAR
                     ALGQLENLHAPKGGVASPRIFHGVAGASGIAMGRAVVIYPPADLESVPDRATEDIDYE
                     LQQFQQALQGVRQEIQNLDSKMANSLMAEERALFEVYLRMLDDNALPEEIINRIHAGN
                     WAQGALRAVIDDHMQSFAAMDDPYLRERMADVRDLGRRLLAHLQQQSSPNRDFPESSI
                     LIGEDISTATLVDMPLENIAAIVTTTGAANSHMAIVARALGIPTVVGVAELPVANLDD
                     AEMIVDAYQSRVYVHPSRELRKRYKEIIKEERQLVAGLDAYKDLPAITPDGHRVTLYV
                     NTGLMADVARGRDRGAEGVGLYRSEIPFMLRDRFPSEEEQRNIYQQQLSAFAPYPVTM
                     RMLDIGGDKDLPYFPIDEVNPALGWRGIRITLDHPEIFMVQMRAMLKAAIGLNNLQIL
                     LPMVSSVFEIEEAQHLLHRAVAEVQEEEKVTITPPKLGVMVEVPSALIQIKDLAHIAD
                     FVSVGSNDLTQYLLAVDRNNARVADLYTPYHPAVLRALQYVVNETHAAGKPVSICGEM
                     AGDPGTAILLMAMGFDSLSMSASNLLKIRRVLRTIKLSDAQELLKEALAIDNAAVIRS
                     MVELELNKLGLWDLVRPTQKPVGGKVPA"
     gene            complement(2417..2899)
                     /locus_tag="PLY05_02010"
     CDS             complement(2417..2899)
                     /locus_tag="PLY05_02010"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HNC02106.1"
                     /translation="MIDAEGFRPNVGIILTNTQGQVLWARRHGHDAWQFPQGGIQTGE
                     TPEQAMYRELWEEVGLEKHHVKILAQTRGWLRYRLPRRYVRYDAPPPVCIGQKQKWFL
                     LSLQSSSDIISLNKCHPPEFDAWQWVSYWYPLNQVVSFKREVYRKTLQELAPRISVTE
                     "
     gene            complement(3246..4232)
                     /locus_tag="PLY05_02015"
     CDS             complement(3246..4232)
                     /locus_tag="PLY05_02015"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012439107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HNC02107.1"
                     /translation="MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEVP
                     VEKAQQALRGVIMELEDCAFPLLAGVIPTDDPYVAFKDADIALLVGARPRGPGMERKD
                     LLQENAKIFTVQGKALNDVASRDVKVLVVGNPANTNAYIAMKSAPDLNPANFTAMLRL
                     DHNRALSQIAAKTGRAVADIENMVVWGNHSPTMYADYRFATVNGDPVKALINDEEWNR
                     DTFLPKVGKRGAAIIEARGLSSAASAANAAIDHIRDWVLGTNGKWVTMGIPSDGSYGI
                     PEGIIYGVPVTCENGTYTRVQGLEVDAFSRERMDFTLNELLEERDGVKHLLP"
     gene            4488..5084
                     /locus_tag="PLY05_02020"
     CDS             4488..5084
                     /locus_tag="PLY05_02020"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004738911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HNC02108.1"
                     /translation="MLIRKLFRFENAHIVRNCTSQRCSRSIHGHSYQVEVILEAHALD
                     HGQMVYDFGLLKGHIKDLIDSFDHAIAFWDKDDAEYISYCQKFSQRWISMPVSPSAEQ
                     FSRLIFVLVDAVLKQTVMQNGEADVVLNSVIVHETQTGYAQAFREDINNPRMGLIKLE
                     DIIFSPQVCHEWTDPEMYEKLKHGEKFVNPTITLQVPI"
     gene            complement(5062..5895)
                     /locus_tag="PLY05_02025"
     CDS             complement(5062..5895)
                     /locus_tag="PLY05_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HNC02109.1"
                     /translation="MRKSCLIIGAAYSIGGAHIGNRRAPFFFRYHAKLPIQWHKIYWQ
                     DSTTLQSYPNLLSSIKRTNRQLIHKICQIRSHFKPIILGGDHSCAIATWQAVSSKPLG
                     LLWIDAHFDSHIPETSFSHRLHGMPLAILLNQGASGLYERNNQVLHKQHTVIFGVHSF
                     ELAEIKLLTQLGIRYYLMSEIHLRGFYQCFDEAWRLVSSAPHGFGVSLDLDSIDPCQM
                     PAVSVRATQGLAFNHLLTAIKKQKKHQLKAIEIAEFNPYHQFHGNNVKKISQLILALA
                     K"
     gene            6000..6746
                     /locus_tag="PLY05_02030"
     CDS             6000..6746
                     /locus_tag="PLY05_02030"
                     /inference="COORDINATES: protein motif:HMM:NF022461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2846 domain-containing protein"
                     /protein_id="HNC02110.1"
                     /translation="MNSIKIGFGTVMLIALTACGTPQKIIHPVSNPELTQLRGKIDYM
                     IDTYRLRAFSIGSYLFPTEGSLFQPIPVTDARNAIVYIYRPHSAWNKEEVISPSVFLN
                     GRRLHGIRDGAYYWMELPAGQYDFAIRRPIGPVYLTHIFKTKLKVEGGRSYYFRYDEE
                     NFRPQPDASLNLFKEKYLTELPEKMALQEIRETRLDKPGWAFATPQQTRWKPFDLYDD
                     GEHPVPEQRLQKEKDLQVAKEVLFWDPMTW"
     gene            complement(6785..7282)
                     /gene="ruvC"
                     /locus_tag="PLY05_02035"
     CDS             complement(6785..7282)
                     /gene="ruvC"
                     /locus_tag="PLY05_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNC02111.1"
                     /translation="MTIIIGIDPGSRLTGYGIIEKKGNKCFFIDAGTIRTESEEMPIR
                     LKRIFEGIQRIVEFHQPTEAAVEKVFLAHNVDTALKLGQARGAAIAALVTRDISVEEY
                     TARQIKQSVVGFGAAQKEQVQQMVTRLLNIPVIPQADAADALAAAICHAHTSQTLLKL
                     AKLKK"
     gene            7430..7729
                     /locus_tag="PLY05_02040"
     CDS             7430..7729
                     /locus_tag="PLY05_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02112.1"
                     /translation="MSIRQIRWFQRTCQMSFLLAALTIFLVFAAIFQLYEISGKTGNV
                     LSLAFVVFFVFGLLLSIFLRTVRCPVCGHTFVGIDDKELFTHTCRNCGRRAGDFG"
     gene            complement(7809..8669)
                     /locus_tag="PLY05_02045"
     CDS             complement(7809..8669)
                     /locus_tag="PLY05_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02113.1"
                     /translation="MNFSEDFIAQLQSLAQQVQNLLPPSPPNIDWQTTKAFRWRLMGK
                     KSYLEAVNDLSPIRLTDLQGIDKQKAEITRNTSQFVNKKPANNVLLTGARGTGKSSLI
                     KALLNEFSDQGLRVIEVDRDLLVDLPDIVQLIAHRPERFIIYCDDLSFTADDASYRAL
                     KTILDGSMHAGSENVLIYATSNRRHLLPEYMSENLQTSVGDDGELHPSEAIEDKISLS
                     DRFGMWLSFYAMSQDTYLDIVEHWLKTYHLELNAAARTAALQWALGRGNRSGRSAAQF
                     AKDWAGKNLI"
     gene            8738..9394
                     /locus_tag="PLY05_02050"
     CDS             8738..9394
                     /locus_tag="PLY05_02050"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC02114.1"
                     /translation="MNSLTKTLAKSSLIFTFLSTLTIAATYDEAILAFENREYDTALE
                     LSLPLAQKQDVDAMTLVGRIYDEGFNQSEKALPWYKKAAELGNAQAQLELAELYEAGD
                     GVKKNKELAIYWYEKAADQDHDEAQLALALHYLEDLEDVPEAIVLLEKSAQQGNAIAQ
                     YRLGLLYLGDSTIKTDKSKAWYYFAMAANKVPEAAQARDVLELEMTPAELKQAMVQNK
                     "
     gene            9611..11146
                     /locus_tag="PLY05_02055"
     CDS             9611..11146
                     /locus_tag="PLY05_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3336 domain-containing protein"
                     /protein_id="HNC02115.1"
                     /translation="MLLTNKVSLLRQALDEANTYEEWKEIALELDSVTGLDLWKLDNE
                     SEHYNHELIRDRLMNLRHLMRQKDDRQLMRALREGLYHDIGNIGNPLLYTYAHIGTKR
                     LIEDYIDQVCSALNYICDANLEFLSLEQKLRFFEDTFHSYGQPALMLSGGATLGLFHV
                     GVCKALHERNLLPKVISGSSAGSLVTGMLGTHTDEELVRMYNGDGFYHHAWTWKRLRD
                     GILGQGFADQRQLERFVRRNIGEYTFEEAYAKTGRHINVTVSPLQAHKARLMNELTSP
                     YLLVWSAALASCAVPVLFPAVTLTTKNHHGEYHPYMPSEKWVDGSVRSDLPRQRLSRL
                     FNVNYFIASQVNPHIVPFMQTDKERLQKGKITSWPKKVARAQLQMVGMGIFDFMRERT
                     SIETIRQLMDHGYGIIGQRYYGDVTIVAKYNWRHYACMLQNPTDEMLMWFRVQGERIT
                     WPKIAMIETHARIGQTLDQCLQRLNKQRENLEQQKAANVIQLQTRSESRKRHTAQIGL
                     LAK"
     gene            11210..12559
                     /locus_tag="PLY05_02060"
     CDS             11210..12559
                     /locus_tag="PLY05_02060"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC02116.1"
                     /translation="MTIQIPGYTIIRELGKGGMATVYLAVQESFGRQVALKVMAPHLA
                     AEPGFAARFDSEAKMVAALSHPHIVTVYDVGSFNNYHYLAMEYHTGGDLSDRIERHDI
                     TPAQALKIIKQLSDALGMAHSKGVVHRDIKPDNVLFRSHNDDAILTDFGIARNMQAES
                     NLTQVGSTVGTPKYMSPEQARGLAVDGRADLYSLGVMLYEMLVGKPPFTASDSISLAI
                     KHCQEPVPRLPEHLHRYQMLVDRLLAKEVKNRFQNGVEVMVAIENLLNNNSSGRVTPV
                     ITASDATPVPLSLDDSKPTKPSANIPSVSVYDRPAANFSLEPTKSKGYEAFFRTSETI
                     GGNFFFKKYSRVVEFSCDDYDEFKEKREQMHDILMDWLNERGKKAVELHVEIQAHPWI
                     HGRIWDALTDDQPFDSPFGMLLEQATVSLHLHDELEPQGGRRILLRDKGKKPAQQPA"
BASE COUNT         3887 a         2344 c         2545 g         3875 t
ORIGIN      
        1 acaaagccta tttgttctac aataagtagg ttttgataag ttttatggaa cggcatgttg
       61 gcactttaag cggcaacatg ccctgttatc ctaagcagga actttaccgc caacaggttt
      121 ttgtgtgggg cgcactaaat cccataaacc aagtttattt aattctagct caaccatgct
      181 acgaatcacg gcggcattat caatcgccaa agcttctttg aggagttctt gggcatcgct
      241 gagtttgata gtgcgtaata cccgacgaat ttttaataaa ttgctggcac tcatacttaa
      301 agaatcaaaa cccattgcca ttaataaaat tgctgtaccc ggatcaccag ccatttcgcc
      361 acagatactt acaggtttgc ctgcagcatg agtttcgtta acgacatatt gtaaggcacg
      421 taacacggca gggtgatatg gcgtgtataa atcagcaaca cgggcattat tacgatcaac
      481 ggctaataag tattgtgtta aatcgtttga gccaaccgag acaaaatcag caatgtgggc
      541 taaatctttg atttgaatca gagcagaggg aacttcaacc ataacgccta actttggagg
      601 cgtaatggtg actttttctt cttcttgtac ttcagcgact gcacgatgca ataaatgttg
      661 tgcttcttca atttcaaata ctgacgacac catcggtaaa agaatttgta gattatttaa
      721 gccaattgct gcttttaaca tggctcgcat ttgtaccata aaaatttcgg gatgatctaa
      781 ggtaatacga ataccacgcc agcctaaagc aggattgact tcatcaattg ggaagtaggg
      841 taagtcttta tctccaccaa tatctaacat gcgcatggtg acaggatagg gggcaaatgc
      901 agataattgt tgttggtata tgttgcgttg ttcttcttca cttggaaaac gatcacgtaa
      961 cataaaggga atttcactgc gatacaggcc aaccccttct gcaccacgat cgcgtcctcg
     1021 tgcaacatca gccattaaac ctgtgttgac atacaaagta actcgatggc catcaggcgt
     1081 aatagcaggt aagtctttat aggcatctaa acctgccact aattgtcgtt cttctttgat
     1141 gatttctttg taacgcttgc gtagttcacg agatggatga acataaacac gagattgata
     1201 agcatcaaca atcatttctg catcatcaag attggcaaca ggtaattccg caactccgac
     1261 taccgtagga atacctaaag cacgtgcaac aatggccatg tgtgagttag ccgcacctgt
     1321 agtagtcaca attgctgcga tattttctaa gggcatatcg actaaagtgg cagtagaaat
     1381 atcttcacca atcaaaatac tgctttcagg aaagtcgcga ttaggcgagc tttgctgttg
     1441 taaatgggct aataaacgtc gtcccaaatc gcgcacgtct gccatacgtt cacgtaaata
     1501 agggtcgtcc atggcagcaa agctttgcat atgatcgtca ataaccgcgc gtaaagcacc
     1561 ttgtgcccaa ttacccgcat gaatacgatt aataatttct tcaggtagag cattgtcatc
     1621 taacatgcgt agataaactt caaacaaggc tcgttcttcg gccattaggc tatttgccat
     1681 tttactatca agattttgaa tttcttgacg tactccttgt aaggcttgtt gaaattgttg
     1741 taattcgtag tcgatatctt ccgttgctct atcaggtaca gactccaaat cagcaggagg
     1801 ataaataacc actgcacgcc ccatagcaat gccactagcg ccagcaacac catgaaaaat
     1861 acgtgggctt gcaacaccgc ctttgggtgc atggagattt tcgagttggc ctaaagcacg
     1921 agcatgagca acaaccccag aaatttgagc cgacaaggtt actaaaaagg cttcttcggc
     1981 ttggccaaaa cgtcgtttgt cttgttgttg aacaactaat acccccaaaa ctttacgccg
     2041 attcataatc ggcacgccta aaaacgagct ataacgttct tcgcctgttt cgggtaaata
     2101 atggtaacgt gggtgagacg cggcatcttc gaggttgatg agttcttctc gttgacccac
     2161 taaacccact aaaccttcag acaaacctaa agacacatta ccaacagcat cagggtttaa
     2221 accatgtgag gccattaata cataacggct attacgttca tctaataaat aaatggaaca
     2281 gacctcggta tgcagtgcat cacgaacact ttgcacgatt acatctaagg cagccaaaag
     2341 cgttggagag ttatccacgt cttgtaccag ctttcttaaa atatccaaca taatcataca
     2401 aagagtctct taattcttat tcggtaacag agatgcgtgg tgccaattct tgtaaggttt
     2461 tacgataaac ctcacgttta aaagatacca cttgatttag cggataccaa taactgaccc
     2521 attgccatgc atcaaattct ggaggatggc atttatttaa actaataata tcactggagc
     2581 tttgcaaact gagtaaaaac catttttgtt tttgaccaat acaaacgggt ggcggtgcat
     2641 catagcgtac ataacgacgt ggtaaacgat aacgtaacca gccacgagtt tgtgctaaaa
     2701 ttttgacatg atgtttctct aagccaactt cttcccacaa ttcgcgatac atggcttgtt
     2761 caggggtttc accagtctga attcctcctt gaggaaattg ccatgcatca tgtccgtgtc
     2821 gtcttgccca taatacttgt ccttgagtat tggttaatat aatcccaaca ttgggacgaa
     2881 agccttccgc atcaatcaca acaaatacct ctgaccataa gaacctaagc ttaggtcatt
     2941 taaaaacaga tttgaaacag gtagataaag cgttttgatc gtcaatattt ataagtgttt
     3001 tagccatgtt gccaaaactt tgattgggct tattggggca aaataaaaga aattacctaa
     3061 gtcctaagat gacgaaatta tagacaaacg ttgttacctg tccgcgtaaa atatcataga
     3121 tgtattcata ccttagacga ggcacttagg gcaagtatta tctgtgtgga ttgcgtaagt
     3181 atcagacggt attaataaaa aaggcctatt tgcatagacc ttttttgaat ggaattagtt
     3241 gagaattatg gcaataagtg ttttacgcca tcacgctctt ctaataattc atttaaggta
     3301 aagtccatac gctcacgaga gaaagcatca acttctaaac cttgaacacg agtataagta
     3361 ccgttttcac aggtcactgg aacaccataa atgatgcctt ctggaatgcc atagctaccg
     3421 tcagaaggaa tacccattgt cacccatttg ccgttagtac ctaataccca atcacggata
     3481 tggtcaatag ccgcgttagc agcagaagca gcagaagata aaccacgagc ttcaataata
     3541 gcagcgccac gcttacctac ttttggtaag aaagtatcac ggttccattc ttcgtcattg
     3601 attaaggctt taacagggtc accattaacg gttgcaaaac ggtagtcagc atacattgtt
     3661 gggctgtggt taccccaaac aaccatgttt tcgatatctg caacagcgcg gccagtttta
     3721 gcagcgattt gagataaagc acgattgtgg tctaaacgta acatcgctgt gaagtttgct
     3781 gggtttaaat ctggagcaga tttcattgca atataagcat tagtgtttgc tgggttgcca
     3841 acaactaata ctttaacgtc acggctagca acatcgttta atgctttgcc ttgtacagtg
     3901 aagattttag cgttttcttg taataagtct ttacgctcca tgcctggacc gcgtggacgt
     3961 gcgccaacta ataaagcaat atcagcatct ttgaaggcaa cataaggatc atcagttggg
     4021 attacgcctg ccaataaagg aaatgcacaa tcttctaatt ccatgatcac accgcgtaaa
     4081 gcttgttgag ctttttcgac tggcacttct aataattgta aaatgactgg ttggtctttg
     4141 cccaacattt caccgctagc gatgcggaat aataagctat aaccaatttg accagctgca
     4201 ccagtaacgg caacgcgaac gggttgtttc attttaagct cctgatgata aaaacacgga
     4261 tgaccgtgac taacaagact tttacctgag cataagtagc taataaagac ttcatgcaga
     4321 gggtattagt tgtaggcttt tctagggcag agaggctcta aaagtaccat aaattaaaaa
     4381 aacgccgcat tgtacctaag cttgagccaa gatgcgaata aaactaataa aatttgtcaa
     4441 atctgttaga ataatcgcct gtctttgctt gtgacgtgtt cagtgttatg ttgattcgca
     4501 aattatttcg ttttgaaaat gcccacattg tacgtaactg tacaagtcag cgttgtagtc
     4561 gctcaataca tggtcatagt tatcaagtag aagttatttt agaagcccat gccttagatc
     4621 atggtcaaat ggtttatgat tttggtttat taaaaggaca catcaaagat ttaattgata
     4681 gttttgatca tgccatcgct ttttgggata aggatgatgc tgaatatatt tcatattgcc
     4741 aaaaatttag tcagcgttgg atcagtatgc ctgtttcacc atccgcagag caattttcac
     4801 gcttgatttt tgttttagtt gatgcggtgt taaaacaaac cgtgatgcaa aatggcgaag
     4861 cagatgtggt gctaaattca gtcattgtgc atgaaacaca aacaggttat gcccaagcct
     4921 ttagagaaga tattaataat ccgcgtatgg gtttaattaa attagaagac attatctttt
     4981 cgccgcaagt ttgtcatgag tggacagacc ctgaaatgta tgaaaaatta aaacacggtg
     5041 aaaagtttgt taatccaact attactttgc aagtgccaat ataagttggc taattttttt
     5101 gacattattg ccgtgaaatt gatgataagg gttaaattct gcaatttcta tcgcttttag
     5161 ttgatgcttt ttttgttttt tgatggcagt tagaaggtga ttaaaagcaa gtccttgtgt
     5221 ggcgcgcaca ctcactgcag gcatttggca tggatcaata ctatctaaat ctaaactcac
     5281 accaaaacca tgtggagcgg agctaacaag tctccaagcc tcatcaaagc attgataaaa
     5341 cccacgtaaa tgaatttcac tcattaaata ataacgaata cctagttgag ttaacaattt
     5401 gatttctgct aactcaaaac tatggacacc aaatatcacg gtatgttgct tgtgtaatac
     5461 ctgattattt cgttcataaa ggccagatgc cccctgatta agtaaaatag ccaaaggcat
     5521 tccatgaaga cgatgactaa aagatgtttc agggatatgg ctatcaaaat gggcatcaat
     5581 ccatagtaat ccaagtggtt ttgagctaac agcttgccaa gtagcaatag cgcaactatg
     5641 gtcaccaccc aaaataatcg gtttaaaatg agaacgaatc tgacaaattt tgtgtataag
     5701 ttgtcgatta gtgcgcttaa ttgaggataa aagattggga tatgattgaa gtgtggtaga
     5761 atcttgccaa taaattttat gccattgaat tggaagtttg gcatgataac gaaaaaagaa
     5821 aggggcgcga cgattgccta tatgtgcgcc accaatagaa tatgcagctc caataattaa
     5881 acaagatttg cgcatttttt tgttttgtcc aataaaaaat cgtatacaat ctgtcggctt
     5941 aaatagattt agactacagg tcgctaagat atgcgagagt ctatatgggg aaagattata
     6001 tgaatagtat aaaaattgga tttggtacag taatgcttat cgcattaaca gcgtgtggta
     6061 ctccacaaaa aatcattcat cctgtcagta atcctgaatt aacacaatta cgtggcaaaa
     6121 tcgattatat gattgatacc tatcgtttgc gtgcatttag tattgggtct tatttatttc
     6181 caacagaagg ctctttgttt caacctattc cagtgaccga tgcgcgtaat gcgattgttt
     6241 atatttaccg tccgcacagt gcttggaata aagaagaagt tatctcccca agtgttttct
     6301 taaatggccg tcgtttgcat ggtattcgtg atggtgctta ttactggatg gaattacctg
     6361 caggacaata tgattttgcc attcgtcgac caatcggtcc tgtttattta acgcatatct
     6421 ttaaaacaaa attaaaagtt gaaggtggtc gttcttacta ttttcgttat gatgaagaaa
     6481 actttcgtcc acaaccagat gcctcactca acttatttaa agaaaaatac ttaactgaat
     6541 tgcccgaaaa aatggcattg caagaaattc gtgaaactcg cttagataaa ccaggttggg
     6601 cgtttgcaac accacagcaa actcgttgga agccttttga tttatatgac gatggagaac
     6661 atcctgttcc agaacaacgt ctgcaaaaag aaaaagattt gcaagttgct aaagaagttt
     6721 tattttggga tcctatgact tggtaattga gccgtttaaa aataggggct acttagcccc
     6781 tattttattt tttcagtttg gcgagtttta gtagggtttg agacgtatgg gcatgacaaa
     6841 tcgctgcggc cagagcatct gccgcatctg cttgtggaat aacaggaata ttaagtaaac
     6901 gggtgaccat ttgttgaact tgttcctttt gtgccgcacc aaaccccacc acagattgtt
     6961 tgatttgtcg ggctgtatat tcttcaacac tgatatcgcg agtgactaat gcagcaatag
     7021 cagctccacg agcttgtcct agttttaagg cagtatcaac attgtgtgct aaaaaaacct
     7081 tttctactgc tgcttcagta ggttgatgaa attcaacaat acgttgaata ccttcaaaaa
     7141 tgcgttttaa tcgaatcggc atttcttcac tttcggtgcg aatagtacct gcatctataa
     7201 aaaaacattt attacctttt ttttcaataa tcccatagcc cgtcaaacga gagcctggat
     7261 caatacctat aataatggtc ataattagtt aatattgtct tactgtaaga catacaaaaa
     7321 tggtgttaat gataagaatt gttacactct caaatctaac ataatgagtt gggtgatata
     7381 gatttaatct attaactcta taattttgtt gattatttag gagtgtttaa tgagtatccg
     7441 ccaaattaga tggtttcaac gtacttgtca aatgagtttt ttgcttgctg cgttaacaat
     7501 ttttttggta tttgcagcaa tttttcagct ttatgaaata tcaggtaaaa cagggaatgt
     7561 attaagtctt gcatttgtcg tattttttgt gtttggctta ttattgagta tttttttacg
     7621 tacagtacgt tgtcctgttt gtggccatac ttttgtgggg atagatgata aagagttatt
     7681 tactcatact tgtcgtaatt gtggcagacg tgcaggcgat tttggttaat taggggcttt
     7741 gcagcgacaa taaaagtggt gcaacaaaaa aggacttaca atatttttgt aagtcctatt
     7801 ggctactttt aaattagatt tttgcctgcc caatctttag caaattgtgc tgcactacgt
     7861 ccagaacgat taccacgacc taacgcccac tgtaaagcag ccgttcgtgc tgctgcgttt
     7921 aactctaaat gataagtttt tagccaatgt tctacgatat caagataggt atcttggctc
     7981 atggcataaa aagatagcca cattccaaat cggtccgata aagaaatttt gtcctcaatc
     8041 gcttcgcttg gatgaagttc cccatcatcg ccaacagacg tttgtaagtt ttcgctcata
     8101 tattcgggca ataaatgtcg gcgattactg gttgcataaa ttagaacatt ttcactgcct
     8161 gcgtgcatac tgccatctaa aatggttttt aaggcgcgat aacttgcatc atccgcggtg
     8221 aaagataaat cgtcacaata aataataaaa cgttcaggac gatgagcgat tagctggaca
     8281 atatctggta aatcaactaa taaatctcta tcgacttcaa ttactcttaa gccttgatct
     8341 gaaaattcat taagtaacgc tttgataagc gaagattttc ctgtgccacg tgcacctgtt
     8401 aacaacacat tattggcagg ttttttgtta acaaattgag aggtattacg agtaatttca
     8461 gctttttgtt tgtcaatacc ttgtaaatct gttaatcgaa taggacttaa atcattaaca
     8521 gcttctaaat aagatttttt tcccatcaaa cgccagcgaa aggctttagt agtttgccaa
     8581 tcaatgttag gaggagaagg gggtaagaga ttttgaactt gttgtgctaa tgattgcagt
     8641 tgtgcgataa aatcttcaga gaaattcata ggagatacat ccaaaaaggt aataattgat
     8701 tataatatcc aaacatcatt tgctagcttg caattttatg aacagtttaa ctaaaactct
     8761 cgccaaatcc tctctgatct tcactttttt atctacattg accattgctg ccacttatga
     8821 tgaagcaata ttagcttttg aaaatagaga atatgacact gcacttgaat tgagtttacc
     8881 attagcacaa aaacaagatg ttgatgccat gactctagta gggcgtattt atgatgaagg
     8941 ttttaatcaa tcagaaaaag ctctaccttg gtataaaaag gccgcagaat taggcaatgc
     9001 tcaagctcaa ttagagttag cagaattgta tgaagcaggt gatggagtta aaaaaaataa
     9061 agaacttgcc atatattggt atgaaaaagc agcagaccaa gatcatgatg aagcacaatt
     9121 agctttagct ttacattatc ttgaggattt agaagatgtg ccagaggcaa tcgttttatt
     9181 agaaaaatca gcacaacaag gtaatgcaat tgctcaatat cgtttaggtt tgttgtattt
     9241 aggtgattca acaatcaaaa cggataaaag taaagcatgg tattattttg caatggcagc
     9301 taacaaagtt cctgaagcgg cccaagcacg agatgttttg gaactagaaa tgacacctgc
     9361 tgaattaaaa caagcgatgg tacaaaataa ataagcctta gcttttcctc aaaaagtagc
     9421 cgcttgtcga ttacatatcc aagaaactat ctagttagat aattgtcgga caatatgctc
     9481 gtcttatcgt tttttttatg ttatagagtt catttgttgt atgattgtat tgtaatgctc
     9541 caacagtgaa gcctattacg aatagagtct aaattgtgat aagaaagcta taggctctcg
     9601 gagaactcta atgttattaa cgaataaagt ctctttatta cgccaagccc ttgatgaagc
     9661 taatacctat gaagagtgga aggaaatcgc tttagagtta gattcagtga ctggtttaga
     9721 tttatggaag cttgataatg agtctgaaca ctataatcat gaattaattc gtgatcgttt
     9781 aatgaatttg cgccatttaa tgcgtcaaaa agatgataga cagttaatgc gtgcattaag
     9841 agaagggttg taccatgata ttggtaatat tggtaatccc ttgctatata cctatgctca
     9901 tattggtact aaacgattaa tcgaagatta tatagatcaa gtttgctctg ctttaaatta
     9961 tatttgtgat gccaatttag aatttttgtc attagaacaa aaattacgtt tttttgaaga
    10021 tacttttcat agttacggtc aacctgcatt aatgttatcg ggtggtgcaa cattgggttt
    10081 gtttcatgtg ggagtgtgta aagccttgca tgaacgtaat ttattaccca aggtaatttc
    10141 tggctcaagt gcaggttctt tagtcacagg tatgttgggg acgcatactg atgaagagct
    10201 agtgagaatg tataatggtg atggtttcta tcatcatgca tggacatgga agcgtttacg
    10261 tgatggcatt ttggggcaag gttttgccga tcaacgacaa ttagaacgat ttgttcgtcg
    10321 taatattggt gaatatacct ttgaagaagc ttatgccaaa acggggcgtc atattaatgt
    10381 cacagtatca ccattacaag cgcacaaagc tcgcttaatg aatgagctaa catcacctta
    10441 tttattggta tggagtgcgg cattagcctc ttgtgctgtt cctgtattgt ttccagcggt
    10501 caccttaacc accaaaaatc atcatggtga atatcatcct tatatgccct cagaaaaatg
    10561 ggtagatggc tctgttcgta gtgatttacc acgtcaacgt ttgtctcgtt tatttaatgt
    10621 caattatttt attgccagcc aagtaaatcc acacattgtg ccatttatgc aaactgacaa
    10681 agaacgtttg caaaaaggta aaattacttc atggcctaaa aaagttgctc gtgctcaatt
    10741 acaaatggtg ggtatgggta tttttgattt tatgcgtgag cgcaccagta tagaaacaat
    10801 acgccaatta atggatcatg gctatggcat tattggtcaa cgttattatg gcgatgtgac
    10861 tattgttgcc aaatataatt ggcgtcatta tgcctgtatg ctacaaaatc cgactgatga
    10921 aatgttaatg tggtttagag tgcagggtga gcgtattact tggcccaaaa ttgccatgat
    10981 tgaaactcat gcaaggattg gtcaaacttt agatcaatgt ctgcaacgac taaataagca
    11041 acgtgaaaat ttggaacaac agaaggctgc aaatgtcatt caattacaaa cacgtagcga
    11101 atcgcgcaaa cgacatacag ctcagatagg attgcttgct aaataactgt aaaaatggta
    11161 tttaccaaga atattttatc taaaaattac agtgaataat tttaacatta tgacaattca
    11221 aattcctggt tataccatta tacgcgagtt aggcaaagga ggcatggcga ctgtttattt
    11281 agctgtgcag gaatcatttg gacgacaagt tgctttaaag gtcatggcac cacatctggc
    11341 tgcagagcct ggttttgcag ccagatttga cagtgaagca aagatggttg ctgctttatc
    11401 tcatcctcat attgtgactg tatatgatgt tggttcgttt aataattatc attatttagc
    11461 gatggaatat catacaggag gagatttaag tgatcgtatt gagcgccatg atattacccc
    11521 cgcacaagct ttaaaaatta ttaaacaatt atctgatgct ttaggtatgg ctcattcaaa
    11581 gggagtcgta catcgtgata ttaagcctga taacgtatta tttagatcgc ataatgatga
    11641 tgctattttg accgattttg gtattgcccg aaatatgcaa gctgaaagca atttaacgca
    11701 agttggctca actgtgggta ctccaaaata tatgagtcca gagcaagctc gtggtttagc
    11761 agtagatggt cgtgcggatt tatatagctt aggtgtcatg ttgtatgaga tgttggttgg
    11821 aaaaccacct tttacagcat cagactccat ttctttagcg attaaacatt gccaagagcc
    11881 tgtgcctcgt ttaccagagc atttacatcg ttaccaaatg ttggttgatc gcttattagc
    11941 caaagaggtt aaaaatcgtt ttcagaacgg cgtagaagtg atggtggcga ttgagaacct
    12001 tttaaataat aatagttctg gtcgcgttac acctgtgatt actgcttctg atgctacacc
    12061 agtaccgtta agtttagatg atagtaaacc aaccaaaccc tcagctaata ttccctctgt
    12121 ttctgtttac gatcggccag ccgctaattt ctctttagag cctacaaaat ctaaaggcta
    12181 tgaagcattt tttagaacat cagaaacgat aggcggcaac tttttcttta aaaaatatag
    12241 tcgtgttgtt gagttttcgt gtgatgatta tgatgagttc aaggaaaaac gagagcagat
    12301 gcacgatatt ttaatggatt ggttaaatga gcgtggcaaa aaagcggttg aattacatgt
    12361 tgaaattcaa gcacatccgt ggatacatgg tcgtatttgg gatgctctaa cagacgatca
    12421 gccatttgac agtccatttg gtatgttatt agaacaggca acggtatctt tgcatcttca
    12481 tgatgagtta gaacctcaag ggggaagacg tattttattg cgtgataaag gcaaaaagcc
    12541 tgcccagcaa cctgcttaat tgcgtaaaat aaagggagta aaactccctt ttttatttgt
    12601 gtctaaatgt tcaacagatc ctatgagtat cgtatgcgtt tattagcttt a
//
LOCUS       DAOQMB010000026        12463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1940_length_12463_cov_17.012734, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000026 DAOQMB010000000
VERSION     DAOQMB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1940_length_12463_cov_17.012734"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="PLY05_02065"
     CDS             complement(<1..847)
                     /locus_tag="PLY05_02065"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC02117.1"
                     /translation="MVFNPELTRDALPPFSMQQQVPTQGAIADYCRFYQLDFEQDFPQ
                     YQHLCGYFDVVGYRLLAHCFYQEKSRGTVWILHGYLEHSGLYKHIIPVLLANHYSVLI
                     YDLPAHGLSSGARASIHHFVEYQKILQHLIAYFDQKLPKPYLALGQSTGGAILMDYVL
                     NQYQAGQKVVFDRLLLLAPLVYPAKMQWLQMKLAFWWFKSTRSGLPRIFRHNTHDQNF
                     IRFMRDEDPLQARWIPLSWLLALKEWIEYIHQFAPCAHPVWVIQGGQDGTVNGEYNIH
                     FIKQRF"
     gene            1001..1654
                     /locus_tag="PLY05_02070"
     CDS             1001..1654
                     /locus_tag="PLY05_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC02118.1"
                     /translation="MIGIPIALLAANGVEWYLHKYALHGVPQEGGGRKSLSPPFMKRH
                     WAHHKQVRLNKYRDDYLYGTENEDAAAEKKALLQICAVTSLAMPIAPFFTLTTYYCAW
                     NYWHVHSQSHLDTEWGKKKIPWHFDHHMNTNQDANWCVTKPWFDYIMGTRVISSPDLQ
                     ESNPLGIKLPKFVENKLNALARRYVPRAFEKLDANMLEEKQKRAQGIENVMPEFQTA"
     gene            complement(1721..2014)
                     /locus_tag="PLY05_02075"
     CDS             complement(1721..2014)
                     /locus_tag="PLY05_02075"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HNC02119.1"
                     /translation="MQKILLLLLIIVSQWTQAQQICELPSTKVKSESININTASLEEL
                     SSKLKGIGKAKAAAIIEWRETNGKFQSLEDVDEVKGIGSATLEKNKALIRFED"
     gene            2351..4078
                     /gene="pilB"
                     /locus_tag="PLY05_02080"
     CDS             2351..4078
                     /gene="pilB"
                     /locus_tag="PLY05_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HNC02120.1"
                     /translation="MAIPSSNNLSFGGLAKRLVNDGLVDATVMQKALIESQKEQVSLI
                     SYLVKNKLAKSAQIAYLLADEFGDPLIDIEAVDIEQLPKEHIDEKIVRQYNLLPFFKR
                     GKRLFVAMGDPTRLEAIDAVRFNTGLNIETVVADEEKLSKIIEKMLDSSSNASMEGLD
                     DDLENLDISGGEDDDKKDSGIVGSGEEEAPIVKYVNKVLLDAIKMGASDLHFEPYEKA
                     YRIRFRIDGVLREYARPPLQLSGKLSARLKVMSQMDISERRVPQDGRIKLKLSKTKAI
                     DFRVNSMPTLFGEKLCLRILDPSSAMLGIDMLGYEPDQKKLFMDALEKPQGMLLITGP
                     TGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNAKVGLTFASALKAFL
                     RQDPEIIMLGEIRDLETAEIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGIPSFNIA
                     TSVNLVIAQRLARRLCSACKRPANYPKPSLLEMGFTEADFEKGFTIYEPVGCDQCRDG
                     YKGRVGVYEVMKVTPKIARIIMEDGNSMEIAEAMEQEGFPNLRRSGLVKVMAGLASLQ
                     EIAKIVGAD"
     gene            4167..5393
                     /locus_tag="PLY05_02085"
     CDS             4167..5393
                     /locus_tag="PLY05_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016653221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNC02121.1"
                     /translation="MATKKADTILTFAYEATDRQGKKIKGEITGTNVALAKAQLRKQG
                     INVQKISKKTNLSFGKRGKKVTPLDIAIFTRQLATMMKAGVPLVQSFEIVADGLDNPT
                     MKEVVMGIKAEVEGGGSFAGALRKFPLYFDDLFCSLVDSGEQSGALETMLDRVAIYKE
                     KSEALKQKIKKAMKYPISVLVVAVIVTAILLLKVVPVFQTMFEGFGAELPAFTMMVVN
                     MSKWLQANWFFALVIILGTIGAFTEAKKRSQAFNNTLDRMALKAPVVGDLVYKSTVAR
                     FARTLATTFAAGVPLVDALDACAGATGNIVYRNAVLKIKDDISSGQQLGFSMRQSGVF
                     PSMALQMVAIGEESGALDAMLDKVATHFENEVDNAVDGLTSMMEPMIMAFLGVVIGGL
                     VIAMYLPIFQMGSVVG"
     gene            5397..6254
                     /locus_tag="PLY05_02090"
     CDS             5397..6254
                     /locus_tag="PLY05_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004659282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNC02122.1"
                     /translation="MLDLLNYSPVLGGVIIFILGICIGSFLNVVIYRLPVMMERGWHN
                     ECCALNNQPPKFTDKFNLSTPASRCPKCGHKIRAWENIPLISWLLLLRGKCSNCHSPI
                     SIRYPLIELATGLLSVLVFIRFGASLQMCTALVFTWVLIALTMIDFDTQLLPDDLTFP
                     LLWLGLLVNLNSTWVSLDKAVLGAVFGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAAL
                     GAWLGPSQLPLIILLSSCVGAVIGGVYMAIRKESAPFAFGPYLAIAGWFAMFGGTEII
                     DWYLGTWKH"
     gene            6255..6854
                     /gene="coaE"
                     /locus_tag="PLY05_02095"
     CDS             6255..6854
                     /gene="coaE"
                     /locus_tag="PLY05_02095"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787215.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNC02123.1"
                     /translation="MYIVGLTGGIGSGKTAASDYLALSYDIEVVDADIVAREIVMQGQ
                     PALQAITDHFGHHILLEDGSLNRAALRQIVFADSQQRKILESITHPAIRAAIQHKLSI
                     STSPYTLLVSPLLFESGQYQFAHRNLVIDVSEELQWQRASTRDNVSAEQIKQIMAAQL
                     CREERLKRADDIVINNGDLAYLHLQLDELHQKYLQLSVV"
     gene            6858..7049
                     /locus_tag="PLY05_02100"
     CDS             6858..7049
                     /locus_tag="PLY05_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HNC02124.1"
                     /translation="MSTTPSTPLMLACRQCQKQTTWHNNPNRPFCSERCKLIDLGSWA
                     SESYSIPVESMPLSEHDHE"
     gene            complement(7065..8465)
                     /locus_tag="PLY05_02105"
     CDS             complement(7065..8465)
                     /locus_tag="PLY05_02105"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HNC02125.1"
                     /translation="MLHRLGVIVSLSWILTACQTPQPLVAPPNNPNPVILPTVPNSVI
                     NLPITIDLETVRAEALKAMPKPLSVGKVTQVLTIANTPLPMESDISHTVYMRDLRLGV
                     TGQDFTATAQLDFAIETKTRAGFLSMGGISCGIGEDLPKIEFTLPGKLYWTATGDLAV
                     QAGQWQLKWLKPCNITAFKFNVEKMANLPLVRDKVQGIVNEQINNALKQVSFKNMLVK
                     TWPQVNEPKQLEKDVWLLLQPEKIGLTDIIGTGRYVKTSLSLTARPQIITGEKPKITL
                     PPMPQPQRLVANGDGFYLALRGDIGLDTANRLLNEKLANKPFDAGGKQVLINSLQLYG
                     SGEKAVLGLRLQQPINAEIFLLGRLTLDVMQNELRLEDIEFELATSSLLAKSANWMLH
                     GTFKNLIAEKARVDFDKDLANVLNDFKDYRQNLGYGATLKAQLANIRPQGVFFTQNDI
                     KAFVVIDGKLGLDISQ"
     gene            8778..10019
                     /locus_tag="PLY05_02110"
     CDS             8778..10019
                     /locus_tag="PLY05_02110"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC02126.1"
                     /translation="MEHFVELKRKLALTLSLAIAFSLLVAFVINYSVSKASVKESLIK
                     NQLPLTASMIATDLQNERLGLIINPRPTDYQKRYLNGIDYGLNKDKIVQMLDLYKKQY
                     GHNVYLTNAKGVIIAHNTGSNIDGQINLTDSPQLQAIFNGLDPKKDGSYEYGFNGENF
                     LVNSSYLEDLDWFLFVDTSEYQATAKIRESFYVNIFIGLFATMAMVWLTYAAINRYQW
                     QINQKLSDMSSSDSLTGLANRYTFDILIHHLLANAKRNQLPVSVVMIDLSSLKQLNHY
                     IHLDSLLQKAGQFIKTSIRASDVGCRWSEDKFLVTFNNCVQDKATAIAESLRVNIEHL
                     LHKEVGDKAHLVSVTAGLTDYYPNDTVETLLERTERALVHAQSQGSSKIMYVQPPYFT
                     PTYMTRRLNKQLDVKAVQMQA"
     gene            complement(9994..10638)
                     /locus_tag="PLY05_02115"
     CDS             complement(9994..10638)
                     /locus_tag="PLY05_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02127.1"
                     /translation="MKTHCSLVHQPRPPYHHGDLARALLKAADEIIEEEGLEAFTLRS
                     CARRAGVSHAAPAHHFKDRAGLLSAYAASIFRDLTACVEEHVQQAGADNYAKLQAVGL
                     AYIRFALQRPSAFSLIFRCESLDKNVDELKEASDACFQVLIDVVQALMPEANKETILL
                     RSMLAWSTVHGFATLWLEGNFAKVYAHQDIEPMCYADTLAQQILQSIKPAFVQP"
     gene            11514..11936
                     /locus_tag="PLY05_02120"
     CDS             11514..11936
                     /locus_tag="PLY05_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AP2/ERF family transcription factor"
                     /protein_id="HNC02128.1"
                     /translation="MGICRIDQPSKSNFGYYVRIRWRGVIYAKFFSDKKHGGPEKAWE
                     AAEAYFHELDAKLPPDSKVGKLTSRNTSGVVGVSRAEGLKRGVPYAHWQAWWTAGGKT
                     KTACFSIRKYGEEEAKQKAIAARKKWEEEYFKENPPTA"
     gene            complement(11993..>12463)
                     /locus_tag="PLY05_02125"
     CDS             complement(11993..>12463)
                     /locus_tag="PLY05_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC02129.1"
                     /translation="GHLDHVGAAKQVAQHYGVKIEGPQKEEQFWLDMLPQQAMMFRFP
                     PAQAFLPDRWLEQGDTVTVGNLQLEVRHCAGHTPGHVVFIHHDSKLAIVGDVLFNGGI
                     GRTDFPRGDYQTLINAIHQQLFTLSDDYQFIAGHGPMSTIGREKKHNPFVGKFG"
BASE COUNT         3880 a         2420 c         2284 g         3879 t
ORIGIN      
        1 caaaacgttg tttaataaaa tgaatattat actcgccatt gaccgtacca tcttgtccac
       61 cttgaattac ccacaccgga tgagcacaag gcgcaaattg atgaatatat tcaatccact
      121 cttttaaagc caacaaccat gacaaaggaa tccaacgcgc ttgtaatgga tcttcatcac
      181 gcataaaacg aataaaattc tgatcatggg tattgtgcct aaagattcta ggcaacccac
      241 tacgggtaga tttaaaccac caaaaggcca acttcatttg caaccattgc atttttgctg
      301 gataaaccaa tggtgctaac aataataatc tatcaaaaac cactttttgt cctgcttgat
      361 attgattgag gacataatcc attaaaatag caccacctgt actttgccct aaagcgaggt
      421 aaggcttagg gagtttttgg tcaaaatagg caattaaatg ctgaagaatt ttctgatatt
      481 cgacaaaatg atgaatgctc gcacgtgcac ctgatgacaa accatgagcg ggtaaatcat
      541 aaattaatac tgaataatga ttagctaaca acacaggaat aatatgttta tataaaccac
      601 tatgttctaa ataaccatgt aaaatccata ccgtaccacg gcttttttcc tgataaaaac
      661 aatgagcaag taatctatag ccaacaacat caaaataacc acataaatgt tggtattgag
      721 gaaaatcttg ttcaaaatct aattgataaa aacggcaata atctgctatc gctccctgcg
      781 ttggcacttg ttgctgcata gaaaacggcg gtaaagcatc tcttgttaac tcaggattaa
      841 ataccatttt gcttaaccat cccacagatt atgatgtgta ctattatctt ataacactta
      901 tcttttcaca cttcaaaatt ggcgcagaat gtcaagttga gttatagagc taatctatac
      961 taaatgtagt acggtagtcc aaaattataa aggtgttgtg atgattggta ttcctattgc
     1021 cttgttagcc gccaatggcg ttgaatggta tttacataaa tatgccttac atggcgtgcc
     1081 gcaggaaggt ggtggccgca aatctttgtc gccacctttt atgaaacgtc attgggcgca
     1141 tcacaaacaa gtacgtttaa ataagtaccg cgatgattat ttatatggca ctgagaacga
     1201 agatgcagcc gctgagaaaa aagcattatt gcaaatttgt gccgttacta gcttagcaat
     1261 gcccattgct ccttttttca ccttaactac ctattactgt gcatggaatt attggcacgt
     1321 tcacagtcaa tcgcatttag atacagaatg gggtaaaaag aaaattcctt ggcactttga
     1381 ccatcacatg aatactaacc aagatgctaa ctggtgtgtc actaaacctt ggtttgatta
     1441 cattatgggt actcgggtga tttcttcgcc tgatttacaa gagtcgaatc ctttaggtat
     1501 taaattgcct aaatttgtag aaaacaaatt aaatgctttg gctcgtcggt atgtgccacg
     1561 cgcttttgaa aagcttgatg ccaatatgtt ggaagaaaag caaaaacgcg ctcagggcat
     1621 cgaaaatgtt atgcctgagt ttcaaacggc ttaataaaga cttcgactcc gctcagtcaa
     1681 cacaaaatgc gccctgagcg gagcaaaggg cagactgccc ctaatcttca aaacggatta
     1741 aggctttatt tttttctaaa gtcgctgaac caataccttt gacttcatca acatcttcta
     1801 aagattgaaa ttttccatta gtttcacgcc actcaataat tgccgccgct tttgctttgc
     1861 caattccttt taatttactg gataattcct ctaatgaagc tgtattaata ttaattgact
     1921 ctgatttaac cttagttgat ggtaactcac atatttgttg agcttgagtc cattgactga
     1981 caatgattaa taacaataac aaaatttttt gcatttactc tctccttgaa ctgattcata
     2041 tttatgtcat caactaccca ctctttgatg agtagatggt gttatcatag caaaaagcat
     2101 ataattttca caaaatatgc gtaggctgct ttgcgcaaac aataagattg atttatattc
     2161 caaaacctta gcaatgtttt ttaataaaaa gctataattc atcatccttt cactacaact
     2221 gtgaggatct gactatcact aagcgactaa acttgctaag ctttatgaca ataacaatca
     2281 atcatcatac tcgtgctttt aatgatgatt aataagctac tctcttgact aacaacggcg
     2341 gaataataac atggctatcc cttcttccaa taacctatct tttggcggtt tagcaaaacg
     2401 actcgtcaat gatggcttgg ttgacgcaac cgtcatgcaa aaggctttaa ttgaatcaca
     2461 aaaagaacaa gtctctctaa tcagttactt agtgaaaaat aaactcgcca aatcagcaca
     2521 aattgcttat ttacttgctg atgaatttgg tgatccgtta atcgatattg aggctgttga
     2581 tatagaacag cttcccaaag aacatattga tgaaaaaata gttcgccaat ataacctatt
     2641 gccatttttt aaacgcggca aacgtttgtt tgtggctatg ggggatccaa ctcgtttaga
     2701 agccattgat gcagtgcgtt ttaacacagg tctaaacatt gaaactgttg ttgcagatga
     2761 agaaaagctc agcaaaatta ttgaaaagat gctcgatagt agcagcaacg cctcaatgga
     2821 aggtttagat gatgacctag aaaacttaga tattagtggt ggcgaagacg acgataaaaa
     2881 agatagtggg attgttggct ctggcgaaga agaagctcct attgttaaat atgtcaacaa
     2941 agtcttgcta gatgccatta aaatgggggc atccgactta cactttgaac cctatgaaaa
     3001 agcttaccgt attcgttttc gcattgacgg agtattacgc gaatacgctc gccccccgct
     3061 acagttatca ggcaaacttt ctgcacgtct taaagttatg tctcaaatgg atatttctga
     3121 acgtcgagtc ccacaagacg gtcgtattaa gctcaagctg tccaaaacca aagccattga
     3181 ttttcgtgtg aactctatgc caacgttgtt tggcgaaaag ctctgtttgc gtattcttga
     3241 cccttctagt gcgatgttgg gtattgatat gttgggttat gaacccgacc agaaaaagct
     3301 gtttatggat gcactagaaa aacctcaagg catgcttctt atcacagggc caacgggttc
     3361 tggtaaaaca gtatcgctat acactggttt aaatattctt aaccgagaag atactaatat
     3421 ttccactgcc gaagaccctg tggaaattaa cttagaaggg attaaccaag ttaacgttaa
     3481 tgccaaagtt ggtttaacct ttgcttcagc attaaaagct ttcttacgtc aagatccaga
     3541 gattatcatg cttggtgaga ttcgtgactt agaaacagcc gaaattgccg ttaaagctgc
     3601 ccaaacaggt cacatggtac tttcaacatt acacaccaat agcgcaccag aaacgttaac
     3661 gcgtttacgg aatatgggga ttccctcatt taacattgca acttctgtaa acttagttat
     3721 tgcccaacgt ctggcacgcc gtttatgtag tgcttgtaaa cgccctgcaa actatcccaa
     3781 accgagttta ctcgaaatgg gctttacaga ggcagacttt gaaaaaggct ttactattta
     3841 tgaacctgta ggctgcgatc agtgtcgaga tggctataaa ggtcgagttg gcgtttatga
     3901 agtgatgaaa gtcacgccta aaattgcacg gattattatg gaagatggca actcgatgga
     3961 gatcgcagaa gcgatggaac aagaaggttt tcctaattta cgacgttctg gtttggttaa
     4021 agtaatggct ggcttagcca gcctgcaaga gattgcaaag attgtaggtg ctgattaact
     4081 gatcactaat acagattaat acgctttact taaaagcaac tcaccacata atataggctt
     4141 gccctaaaaa atgagtatga cggattatgg caacaaaaaa agctgacaca attttgacct
     4201 ttgcttatga agcaacagat cgacaaggta aaaaaatcaa aggtgaaatt actggtacta
     4261 acgtcgctct tgctaaagca caattgcgta agcaaggtat aaatgttcag aaaataagca
     4321 aaaaaaccaa cttatcattt gggaagcgag gtaaaaaagt taccccgtta gacattgcta
     4381 tttttactcg tcagttagca acaatgatga aagcaggtgt accattagta caatcatttg
     4441 aaattgttgc tgatggtctt gataatccca ccatgaaaga agtggtcatg ggtattaaag
     4501 cagaagtaga aggtggtgga agttttgcag gagcattgcg taagttccct ttatattttg
     4561 atgatttatt ttgtagctta gtagactctg gtgaacaatc tggtgcttta gaaaccatgc
     4621 ttgaccgcgt cgctatttat aaagaaaaaa gtgaagccct aaaacaaaaa atcaaaaagg
     4681 caatgaaata tcctatcagt gtgcttgtgg ttgccgttat cgtaacagca atattgttat
     4741 taaaagtagt ccctgtattc caaacaatgt ttgaaggttt tggtgcagaa ttgccagcat
     4801 ttactatgat ggttgttaat atgtctaaat ggttacaagc caattggttt tttgcactag
     4861 tgattatttt aggaactata ggtgcattta ctgaagcaaa aaaacgtagt caagctttta
     4921 ataatacttt ggatcgcatg gccttaaaag cgcctgttgt gggtgattta gtttataaat
     4981 caacagttgc ccgtttcgcc cgtactttag ctacaacatt tgcggctggt gtgcctttag
     5041 ttgatgctct tgatgcctgt gcaggtgcaa caggtaatat tgtttaccgt aatgcggttt
     5101 taaaaattaa agatgatatt tcttctggcc aacaactcgg tttttcaatg cggcaatcgg
     5161 gtgtatttcc ttcaatggct ctacaaatgg ttgctattgg tgaagaatct ggtgctttag
     5221 atgctatgct tgacaaagtc gccactcact ttgaaaacga agttgacaat gcagtggatg
     5281 gtttaaccag tatgatggag cctatgatca tggcattttt aggtgtagtt attggtggct
     5341 tagttatcgc gatgtatctg ccaattttcc aaatgggttc cgtggtaggt taagttatgc
     5401 tagacctcct caattactcc cctgtcttag ggggagtaat tatttttata ttaggtatct
     5461 gtattggtag ctttttaaat gttgtcattt atcgcctacc ggtcatgatg gaacgaggtt
     5521 ggcataatga atgctgtgca ttgaataacc aacctcctaa atttactgat aaatttaatc
     5581 tttctactcc tgcttcacgt tgtcctaaat gtggtcataa aattcgtgca tgggaaaata
     5641 ttcctttaat tagttggctt ttactgcttc gtggcaaatg tagcaattgc cacagcccca
     5701 tttcaatacg ttatccattg attgaattag caacaggctt attatctgta ttagtcttta
     5761 tacgctttgg tgcaagtctt caaatgtgta cggcactcgt gtttacatgg gtattaatcg
     5821 ctctcactat gattgatttt gatactcaat tgctacctga tgatttaacc tttcctctgt
     5881 tatggcttgg cttattagtt aaccttaatt caacatgggt aagtttagac aaagccgttt
     5941 taggcgctgt gtttggttat ttatcgctat ggagtgtcta ctggttattt aagttagtaa
     6001 caggtaaaga aggcatgggc tatggtgatt ttaaactttt ggctgcttta ggtgcgtggt
     6061 tggggccaag tcaattacca cttattatat tattatcttc atgtgttggt gcagtaattg
     6121 gtggggtgta tatggctatc cgtaaagaaa gcgcgccatt tgcatttggc ccttatttgg
     6181 caattgctgg ttggtttgcc atgtttggtg gcacagaaat tattgactgg tatttgggta
     6241 cgtggaaaca ctaaatgtat atagtcggac tgacgggtgg tattggcagt ggcaaaactg
     6301 cagctagcga ttatttagca ctaagctacg atatagaagt agttgatgca gatattgttg
     6361 cccgtgaaat tgtgatgcaa ggacaaccag ccttacaagc tattacggat cactttggcc
     6421 atcatattct attagaagat ggttcattaa atcgtgcagc attacggcaa atcgtttttg
     6481 ctgattctca acaacgtaaa atacttgagt caatcactca tcctgcaatt cgtgccgcca
     6541 ttcaacacaa attaagcatt agtacatctc cttatacttt attagtttca ccattactct
     6601 ttgaaagtgg tcaatatcaa ttcgcccatc gcaatctggt aattgatgtc agtgaagaat
     6661 tacaatggca acgtgcttca acacgtgaca atgtttctgc tgaacaaatt aaacaaatta
     6721 tggcagcaca actttgtcgt gaagagcgtc taaaacgtgc ggatgacatt gtcatcaata
     6781 atggcgattt ggcttatttg catttacaat tagatgagct acatcagaaa tacctacaac
     6841 tatcagtcgt ataaatcatg tccactactc cctccacccc cttaatgtta gcttgtcgcc
     6901 aatgtcaaaa acagactaca tggcacaata atccaaatcg ccctttttgt agtgagcggt
     6961 gtaaattaat tgatttagga tcatgggcaa gtgaaagtta tagcattccc gttgagtcaa
     7021 tgcccctttc agagcatgac cacgaataat aagatagcac ctaattactg actaatatct
     7081 aagcccaatt tcccatcaat caccacaaac gctttaatat cattttgtgt aaaaaacaca
     7141 ccttgaggac gaatgttggc taattgtgct tttaatgtag caccataacc taaattttga
     7201 cgataatcct taaaatcatt taagacattg gctaaatctt tatcaaaatc aactcgtgct
     7261 ttttcggcaa ttaagttctt aaatgtacca tgcagcatcc aatttgctga tttagctaac
     7321 aaactactgg ttgccagctc aaactcaata tcctctaagc gtaactcatt ttgcatcaca
     7381 tccaatgtta atctgcccaa taaaaatatc tcagcattaa tgggttgttg caaacgcaaa
     7441 cctaaaacag ctttttctcc actgccatat aactgcaagc tatttattaa tacttgtttg
     7501 cctccagcat caaagggttt attggccaat ttttcgttga gcaagcgatt cgctgtatct
     7561 aaaccaatat caccacgcaa tgccaaataa aaaccatcac catttgccac cagacgttgt
     7621 ggttgaggca tgggtggcaa agtaatttta ggcttttcac cagtgataat ctgtggacgt
     7681 gcggttaagc tcaaactagt cttcacataa cgtccagtcc caataatatc ggttagacca
     7741 attttttcgg gctgtaataa taaccaaaca tctttttcta attgtttagg ttcgttgact
     7801 tgtggccatg ttttaaccag catattttta aaactaactt gctttaaagc attattaatt
     7861 tgttcattca caattccttg cactttgtcg cgtaccaaag gcaaattagc cattttttca
     7921 acattaaatt taaaggcagt aatgttacag ggttttaacc atttaagttg ccactgccct
     7981 gcctgcaccg ccaaatcgcc cgttgctgtc caatataact ttcctggtaa ggtaaattca
     8041 attttgggta aatcttcacc aataccacaa ctaatccccc ccatactcaa aaagccagca
     8101 cgcgttttgg tttcaatcgc gaagtccaat tgcgcggttg cagtaaaatc ttgaccagtt
     8161 acccctagcc gtaaatcacg catatacacg gtatgactaa tatctgattc catcggtaaa
     8221 ggtgtattag caatcgtcaa aacttgagtc actttaccca ctgacaaagg tttaggcata
     8281 gcttttaatg cttctgctcg taccgtttct aaatcaatcg taattggcaa attaatgacc
     8341 gaattgggta ctgtgggcaa aattacagga tttgggttat tcgggggagc aactaagggt
     8401 tgtggcgttt gacaagcggt caatatccac gataaagaca caatcacacc aagacgatgc
     8461 aacatttttt actcctagag agtcaacaac taacttatga atgtcataaa gcttaagaaa
     8521 ttgtttaaaa attctacttt ttatttgtca aacaatcatt cttaatctac actttttgcg
     8581 acgcagttta gcgcatataa aaaacattag cgcgcgtata gcataagtcc aaagctcatc
     8641 attctgacac acttatacgc cttagtttaa aactacgtca gacaaaactg tgtatctttt
     8701 tttattgtct acgctaagat tgtccttgaa atgtaggttt tcagacaatt tggcagtttg
     8761 ctcgctaggg atatattatg gaacactttg tagagctaaa gcgtaaactt gcgcttactt
     8821 taagtcttgc cattgctttt tcattactgg tcgcttttgt gatcaactac agtgtctcaa
     8881 aagcttcggt aaaagagtct ttaatcaaaa atcaattgcc tttaactgct agcatgatcg
     8941 ctactgactt acaaaacgaa cgattaggct tgattatcaa cccacgtcct accgattatc
     9001 aaaaacgcta tttaaatggc attgattatg gtcttaataa agataaaatt gtgcaaatgc
     9061 ttgatcttta taaaaaacaa tatggccata atgtttatct aaccaatgcc aaaggtgtga
     9121 ttattgccca taatacgggt agtaatattg acggccaaat taatctaact gactccccac
     9181 aattacaagc aatctttaat ggtcttgatc ctaaaaaaga tggctcttat gaatatggat
     9241 ttaacggcga aaacttttta gtaaatagta gttatttaga agatttagac tggtttttat
     9301 ttgttgatac cagtgaatat caagcaactg ccaaaattcg tgaaagcttt tatgtcaata
     9361 tttttattgg cttgtttgcc acaatggcta tggtttggtt aacctatgct gcaattaatc
     9421 gatatcaatg gcaaatcaat caaaaactca gtgatatgtc tagctctgat agcttaacag
     9481 gcttagctaa tcgttatacc tttgatattt taattcatca tttattagcc aatgccaaac
     9541 gtaatcaact tcctgtgtct gtggtgatga ttgatttatc ctcactcaaa caactcaatc
     9601 attatattca tttggatagc ctattacaaa aagcagggca atttattaaa acatctatta
     9661 gagcatcaga tgttggttgt cgctggagcg aagacaaatt tttagtgact tttaacaatt
     9721 gtgttcaaga caaagcaaca gcaattgctg aatcattacg tgttaatatc gaacacttat
     9781 tacacaaaga ggtcggcgac aaagctcatt tagttagtgt cacggcaggt ttaactgatt
     9841 actatcccaa tgatactgtt gaaaccttac ttgaacgtac agagcgcgct ttagttcatg
     9901 cccaaagtca aggtagtagt aaaattatgt atgttcaacc gccctatttt actccaacct
     9961 atatgacacg tcgccttaat aaacaattgg atgttaaggc tgtacaaatg caggcttaat
    10021 actctgtaaa atttgttgag ccaatgtgtc agcatagcac attggctcaa tatcttgatg
    10081 tgcataaacc tttgcaaaat taccctctaa ccataatgtc gcaaaaccat gtacagtact
    10141 ccaagccaac atacttctta atagaatagt ttctttattt gcctcaggca ttaacgcttg
    10201 caccacatca ataagcacct gaaagcaagc atcactcgcc tcttttaatt catcaacatt
    10261 tttatccaaa gattcacaac gaaaaatcaa actaaatgca ctaggtcttt gtaaagcaaa
    10321 acgtatatat gccaatccta cagcttgtaa ctttgcataa ttatctgcac cagcttgttg
    10381 cacatgttct tctacacaag ccgttaaatc acgaaaaata cttgcagcat aggcagataa
    10441 taaacccgcc ctatccttaa aatgatgtgc aggcgcagca tgcgaaacac ctgcacgtct
    10501 ggcacaagaa cgtaaggtaa atgcttccaa tccctcttct tcaataattt catctgccgc
    10561 ttttaataat gctcttgcta aatcaccatg atgataaggc ggtctaggtt gatgaactaa
    10621 agaacaatgc gtcttcataa cttaccttaa aaataaaaat caatttaacc aactagaaaa
    10681 atattctata taaatatttt acaacacgcc aagacgatta atcgttaaat attattaaat
    10741 agtcctaaca caaataaata agatattttt gtaactaact aagtttataa caaacataat
    10801 cattattgct ttaaactttc acaaattaaa tttattaatc atgattagga attacatagt
    10861 tattggtatt tttattgaca tatcaaccgt gttcaataat tttatgttcg aagatcagac
    10921 gtatgaaaaa aacaccctgc tctcaaatta ctaacgtttt gctaattcat gattttgttg
    10981 aaaattgtca tattaatttt agtgcctaga caatgactgt atttaattta aaaaatagaa
    11041 tttttctgac tattagacat aaattataac gattataagt actttttttg actttttaac
    11101 ccaatagaaa atgcaccttt gttatattta ttgtacaata acaacgcgct gccatattta
    11161 tttataaaaa acgtagatgg attgcacatt actaactaaa tattttgtta ctcactcaca
    11221 agttatttag ttagcacatc cagtcatggc aacgccctca ttaagtgtat tggtgtaaat
    11281 caatcattta taggaactgt atgttattaa tgtgatatct acgaggacac acaaacacga
    11341 agtttgttat ctctcgtcgc aaggttggaa aaacgcgata aggccatgaa cggcaaggtg
    11401 ttttgatact caatcaaaac gtaatgctag gttattttga ttggtggcct atcaatttta
    11461 ttgatatttc ccatgatata attaaccctt ttctcagttc gtgagtaatt gttatgggta
    11521 tttgtcgtat tgatcaaccc tcaaaaagca actttggtta ttatgtgcgc attagatggc
    11581 gtggcgtgat ttatgcaaag tttttctctg acaaaaaaca tggtggccca gaaaaggctt
    11641 gggaagccgc agaagcttat tttcatgagc tagatgctaa acttccacct gatagtaaag
    11701 taggcaaatt aacgtcgcgt aataccagtg gtgttgtcgg tgttagtcgc gctgaggggc
    11761 taaaacgcgg tgttccgtat gcacattggc aagcttggtg gacagcagga ggcaaaacca
    11821 aaactgcttg tttttctatt cgcaagtatg gcgaagaaga ggccaaacaa aaagccattg
    11881 cagcacgcaa aaaatgggag gaagagtatt ttaaagaaaa cccacctacc gcttaattga
    11941 gcgtgtattt ttgtgatacg gggttgttgg atttatccaa ccccctcacg acttaaccaa
    12001 acttacccac aaatggatta tgctttttct ctcgaccaat cgtcgacatt ggcccatgcc
    12061 ctgcaataaa ctgataatca tcacttaagg taaataattg ctgatgaatc gcattaatca
    12121 aggtttgata atcccctcta ggaaagtccg ttcgtcctat gccaccatta aacaacacat
    12181 cgccgacaat cgctaattta ctatcatggt gaataaatac cacatgaccc ggtgtatggc
    12241 ctgcacaatg gcgaacttct agttgtaaat ttcccactgt tacagtatcc ccctgctcta
    12301 accaacgatc gggtaaaaat gcttgtgctg gcggaaatct aaacatcatg gcttgttggg
    12361 gcaacatatc caaccaaaat tgttcttctt tttgaggacc ttcaatctta acaccataat
    12421 gttgagcaac ttgttttgct gctcctacat ggtctaaatg gcc
//
LOCUS       DAOQMB010000027        12240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_1988_length_12240_cov_22.346738, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000027 DAOQMB010000000
VERSION     DAOQMB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1988_length_12240_cov_22.346738"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1817)
                     /locus_tag="PLY05_02130"
     CDS             complement(18..1817)
                     /locus_tag="PLY05_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02130.1"
                     /translation="MPQSFPFSQQELNKYANQKVLILFERLSDQQKQWVWHFIQLIKK
                     QFPNADHYAVTGSGSVPYMSIGQKSKTAQKGYPFFNIYYSHQHPHIWVNSSRLPDDKI
                     KKKDRDLSFDQHSLDQMNEWFERMVTVLEKSQLSINGLGLIPKDYGDITIEEDSQGHP
                     PNELIQLTCDMSKLNQILYGPPGTGKTYATIDEALKILDPSFYEGVNLDNTLNQEDKR
                     IQLKARFDDLVEDKQVRFVTFHQSFSYEDFVEGIRASSEEGQLKYKVEDGVFKTICAS
                     AEARVVKQEHEPIELKNRRIWKMSLGEASSSDESYIYDECIEKNHILLGYGGVSDFSH
                     CKNKADITSVFEKSGSKFKDYAVTAVEIFLLKLQIGDLVVISDGNSKFRAIAEITGNY
                     KCLSREVEDDGYGQCREVRWLQIYAKSRPADDILDKNFMMKSIYRLRDGSINLSKLSK
                     LLNPVDTTLDQSDINTAKVLIIDEINRGNISRIFGELITLIEPSKRAGEDEALEVTLP
                     YSKDPFSVPNNVYLIGTMNTADRSLAGVDIALRRRFSFIEMPPKPELLKEVIVEGVDI
                     EKLLTVMNQRIEVLLDRDHCLVILARKLDSCSL"
     gene            2300..2512
                     /locus_tag="PLY05_02135"
     CDS             2300..2512
                     /locus_tag="PLY05_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02131.1"
                     /translation="MSIEKVSPQDWQKARQQKPMTQVEVAKLLHCSIDSVKSWDIGRN
                     PMGRALFNYFLLVTDQHPTQRIEQKI"
     gene            2543..3418
                     /locus_tag="PLY05_02140"
     CDS             2543..3418
                     /locus_tag="PLY05_02140"
                     /inference="COORDINATES: protein motif:HMM:NF021637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HNC02132.1"
                     /translation="MSDKILKDMIKKMENYGNKVSTSAPPPEENVSDALSDYFVCDLQ
                     NYSFKDDSTTMEAPIFSLSTQEDKQIWLWTSEDGKKTVEVAPSFYGRATLFDKDVLIF
                     AASQLIAAINKGEKPSKTIRFKAADYFKATKRGTGGNEYERFKTSLDRLSGTRIKTNI
                     KTDNTKKTNGFGIIDGWSVIEKKERNDQMVAVEITLSEWLYNTIIEKDVLTITPQYFE
                     IRKPLERRLYEIARKHVGKQDYWKISLDKLKGKCGSIRELRKFRLDIKNICVSDNIPD
                     YSLALADDIITFIKK"
     gene            4660..5064
                     /locus_tag="PLY05_02145"
     CDS             4660..5064
                     /locus_tag="PLY05_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02133.1"
                     /translation="MSANKKTAFFSNKSLNILGSFSSGRLNAIIERYENLIQIIKIDD
                     IFLDNEFQLLYTVCKESAIKERTDWQEFKIPSLIEKINNHPCEFETSEIILKLKNMNS
                     LQELSLIEKIERYWLEERVKLFNEYRNKKNPL"
     gene            5194..6714
                     /locus_tag="PLY05_02150"
     CDS             5194..6714
                     /locus_tag="PLY05_02150"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNC02134.1"
                     /translation="MNDKINQDSINKALWSACDTFRGTISADTYKDFILTMLFLKYIS
                     DVWQDHYDGYKAEYGDEPELIEEMMKNERFVLPKAASFYALYERRHEAGNGERIDQAL
                     HAIEEANGTKLKDAGKSVFQDISFNTDKLGEEKQKNTILRHLLEDFAKPELNLKPSRV
                     GTLDVIGNAYEYLIKNFAASGGQKAGEFYTPPEVSDLIAELLDPQPGDSICDPACGSG
                     SLLMKCGRKVVANHGSKQYALYGQEAIGSTWSLAKMNMFLHGEDNHKIEWGDTIRNPK
                     LLDKNGNLMLFDIVTANPPFSLDKWGHDEAEHDKFSRFRRGVPPKTKGDYAFILHMIE
                     TLKPKSGRMGVVVPHGVLFRGSSEGNIRQKLIDENLLDAVIGLPEKLFYGTGIPAAIL
                     IFKKNKRDNNVIFIDASRDFKAGKNQNLLTEENIAKIIATYHACNNVDKYAYVASLDE
                     IKENDYNLNIPRYVDTFEEEEEIDLMAVRAEREQLQAQLTELETEMAKYLEELGYE"
     gene            6707..7729
                     /locus_tag="PLY05_02155"
     CDS             6707..7729
                     /locus_tag="PLY05_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNC02135.1"
                     /translation="MNKPSLQHYDRLIDEISGIFIQGQARTIQAINAGLVMTYWQIGQ
                     HIVEFEQQGQASAEYGTQLLANISRDLRLKHGKGFSLSNLKRFRQFYLAYRNGATVSH
                     QLSWSHYVELLKISDETERSFYTNQAQSANWSVRELKRQKDTGLFLRLATSKNKSEIL
                     ELANRHALITKPEDVQRDSYVFEFLNLPERENYSERELETALCEHLEHFLLELGKGFT
                     FVGRQYRITLNNTHYKVDLVFYHRILRCFVLIDLKINDVKHHDIGQMNMYMGYFAAEE
                     NIEGDNPPIGIILSRDKDELLVEYATYGMDTSLFVQKYQLYLPNEQELRREIEATLAE
                     QEKNNE"
     gene            7783..8991
                     /locus_tag="PLY05_02160"
     CDS             7783..8991
                     /locus_tag="PLY05_02160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC02136.1"
                     /translation="MLPKGWTLTSLREVCSGNLQTGPFGSQLHAHEYVPTGIPVLMPK
                     DLIDCRADLIGAAKITQAKANSLGKHVLKVGDLLFSRRGDVSRFALIDSDSEGALCGT
                     GCLKARPNSQHSSKFLAYFIQKESVKKWLEQNAVGQTMPNMNTEILGELPLIAASSKE
                     EEEKIAQILSTWDKAITTTEQLLANSQQQKKALMQQLLTGKKRLLDNNGVRFGGEWKR
                     VELGDLLDYQQPTPYLVESTEYSDDFSTPVLTAGKTFILGYTDEESGIFSEKLPAIIF
                     DDFTTDSKFVDFPFKAKSSAMKILTAKKGVSIKYVYEAMQLLDFAVGGHQRHWISIYS
                     RLVIPFPATDEQQKIAAILSTADQEITALQQKLDALKQEKKALMQQLLTGKRRVQLND
                     QNSKAITYGN"
     gene            8981..10351
                     /locus_tag="PLY05_02165"
     CDS             8981..10351
                     /locus_tag="PLY05_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004662900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02137.1"
                     /translation="MEIKQFRLANLGRFSELEVKLAPTPELKSNVTVFIGNNGAGKTS
                     ILKALATSLSWFVARLRSEKGSGSPIPEGVILNGQASALIEVTVFDDHKQSNHSDSKI
                     EKAMYQWSLAKTQTSKKAKFNSNLNDASRLADFYRTALTQNEKLGMPLIAFYPVERVV
                     LDVPLKIKERHSFLQIDGYENSLNQGVDFRRFFEWFREREDSENESGLPQDVMDKLLE
                     TLGDNNELWHKLQSLMASSRDRQLTAVRTAISNFMPGFSHLRVRRKPRLHMSIDKNGQ
                     TLNVLQLSQGEKSLMALVGDIARRLAMMNPALENPLHGDGVVLIDEVDMHLHPTWQRS
                     IIERLTSTFPNCQFILSTHSPLVISDYKDVLVYSLDDGEVSQVPSQYGEDANTVLLDV
                     MNTNIRNAVINVRINDLRDLIEDGRLNEAQALLHELEGELPAKNMELAKARLLLRKEE
                     LRHAKD"
     gene            10338..10979
                     /locus_tag="PLY05_02170"
     CDS             10338..10979
                     /locus_tag="PLY05_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02138.1"
                     /translation="MRKIKKGIEPASLTTFKRNMPHARYPDLTVLERQDIRQACTEEQ
                     FYLCAYCCQAISGSDQDTMNEHVEAQQLAPNRTLDYMNVVASCTTKQQCDAAHGSQPL
                     PLTPLMNECETELRFMLSGRVQGLSDRAREAIRVLNLGDTEHNNKALIEKRKQLVDGL
                     IWANYGSDPARLQLEEDEELIQMLIDDLELPKNGKLEPFAPVLSNILRNRLAA"
     gene            11059..12186
                     /locus_tag="PLY05_02175"
                     /pseudo
     CDS             11059..12186
                     /locus_tag="PLY05_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
BASE COUNT         3825 a         2425 c         2533 g         3457 t
ORIGIN      
        1 aaaagcatct actactttta gagggaacaa ctgtctagtt ttctggctag gatcactaaa
       61 caatggtcac ggtctaataa tacttcaata cgttggttca ttacagtcaa cagcttttca
      121 atatccactc cctccacaat cacctctttt agtaactctg gcttgggggg catttcaata
      181 aacgaaaaac ggcgacgtaa ggcaatatcc acccctgcta aagaacggtc agctgtattc
      241 atagtaccta ttaaatagac gttatttggt acactaaatg ggtcttttga atacggaagc
      301 gtaacttcta aggcttcgtc ttcgcctgct cgttttgagg gttctattag ggttattaac
      361 tcaccaaaaa tacgcgatat atttcctcgg tttatttcgt caataattaa taccttagca
      421 gtatttatat cgctttgatc gagagtagta tcaactggat ttagtaattt gctaagtttt
      481 gaaaggttaa ttgatccatc ccgtaatctg taaatcgact tcatcataaa gttcttgtcc
      541 aaaatatcat cagcaggacg ggattttgca taaatttgca accatctaac ctcccgacat
      601 tgaccatatc catcatcttc aacttcacga ctcaggcatt tatagtttcc tgttatttcg
      661 gctattgctc taaatttgct gttgccatca gaaataacga ccagatcacc aatttgtagt
      721 tttaataaga atatctccac cgcggttacc gcataatctt tgaatttaga acctgacttc
      781 tcaaataccg atgttatatc agctttattt ttgcagtgcg aaaaatcact tacaccacca
      841 tatcctaata aaatatgatt tttttctata cattcatcat aaatatatga ttcatcacta
      901 ctactggctt cgccaagtga cattttccat atacgacgat ttttaagctc tattggctca
      961 tgttcttgtt taactactct tgcttcggct gaagcacaaa tcgttttaaa taccccatcc
     1021 tctactttat atttaagctg accttcctca gaacttgctc taataccttc tacaaaatcc
     1081 tcataactaa aactttggtg aaaggttaca aagcggactt gtttgtcttc tactaagtca
     1141 tcaaagcgag cttttaactg aatacgtttg tcttcttgat ttaaggtatt gtctaaattc
     1201 accccttcat aaaaactagg atcaagaatt tttaatgctt catcaatagt tgcataggtt
     1261 ttgcccgtac ctggagggcc atatagaatt tgattgagct ttgacatatc acacgttagt
     1321 tgtattagtt cattaggtgg atgcccctga ctatcctcct caattgttat atcgccataa
     1381 tctttaggta ttaatcctaa accgtttata gataactggg atttttctaa tacagttacc
     1441 attctttcga accattcatt catttgatcc aaggaatgct gatcaaaact taaatctcga
     1501 tctttctttt taattttgtc atctggtaaa cgactgctat ttacccaaat atgaggatgt
     1561 tgatgactgt aataaatatt aaaaaatggg tacccttttt gagccgtttt gcttttttga
     1621 ccaatagaca tataagggac actgccactg cctgttacag catagtggtc agcgttgggg
     1681 aattgctttt taatcaattg aataaaatgc caaacccatt gtttttgttg atcactaaga
     1741 cgttcaaata aaattagcac tttttgattg gcatatttgt tcaattcttg ttgcgaaaaa
     1801 ggaaaacttt gtggcatttt caatccctaa taaattttac taacatccta tcttcatatc
     1861 ataatttttt tattgaatcg agatgttatt ataaaaatga tattctctcc attttatata
     1921 tatctttgca ctaaatacgc tgtagccact aatgtttcat tatgtacttt tgctgatgcc
     1981 gtcgtttctt tccatcgctt cgcttaaaat tataagcaat tttatacact ccttaattat
     2041 ttgttgaata aaatttactg ataaattcta gaccctcgat acaaaattgt atgctctaaa
     2101 aactttttat agtagaagtg tgtagtaaat aataaacagg tattcaactt taattactat
     2161 gataactccc cttacaaagc aatacgtaac aaatatatcc tcattattct tccatttcag
     2221 ccaatgtggg tattaccatc ttgtgagcat gctggcaaat gtataatata caccaaccac
     2281 gtaacatgaa agcagtgaaa tgtcgataga gaaagtaagt ccccaagact ggcaaaaagc
     2341 aagacaacaa aagcccatga ctcaagttga agtcgcaaaa ttattgcatt gctcaattga
     2401 ttcggtaaaa agttgggata ttggtcgtaa tcctatgggg cgtgcattat tcaactattt
     2461 tttactggta accgaccaac atccaactca acgtatcgag caaaaaattt agtttaagct
     2521 ttagtctata aggagtgtat aaatgtcaga taaaatacta aaagacatga taaaaaagat
     2581 ggaaaattat ggcaataaag tgagtacttc tgcccctcca cctgaagaaa atgttagtga
     2641 tgctctaagt gattattttg tatgtgattt gcaaaactac tcatttaaag atgactcaac
     2701 gacaatggag gctccaattt ttagtttatc gacacaggaa gataagcaga tttggctatg
     2761 gacaagcgaa gatggaaaaa aaacagttga agttgctcca agtttttatg ggagagccac
     2821 tttattcgac aaagacgttt taatttttgc ggctagtcag ctaatagctg caataaacaa
     2881 aggtgaaaaa ccatctaaaa ctattcgttt caaagcagca gactatttta aagcaaccaa
     2941 aagaggtact ggtggaaatg aatatgaacg gttcaagact tcattagata ggttaagtgg
     3001 aactcgaata aaaaccaata taaaaacaga taacacaaaa aaaactaacg ggtttggtat
     3061 tattgatggc tggagtgtta ttgaaaaaaa agaaagaaat gatcaaatgg tagctgtaga
     3121 aattacatta tcagagtggc tttataatac tattattgaa aaggatgtct taacgataac
     3181 tcctcaatat tttgaaatta gaaagccttt agaaagaagg ctatatgaaa tagctagaaa
     3241 acatgttgga aaacaagatt actggaaaat atccttagac aagttaaaag gtaaatgcgg
     3301 cagtattcgc gaattaagaa agtttcgctt agatattaaa aatatttgtg tatcggataa
     3361 tatcccagac tatagcttag cattagccga tgatatcatt acatttataa aaaaatgata
     3421 gtatttattt tcaaaatcct ctcattgagg ggattttttg atattctcaa taacctcccc
     3481 tttcttcggg gtttcgcgga cgtttttggc caaaaaaaca accaaaacca tagcattcat
     3541 ttaataaata acgacaattt atcgccattt tagggcaaat ccactaagaa aaatgccttt
     3601 tttaaggctt tattccttcg gggtttcgcg gacacttgaa cggtgtttcg cggacgtttg
     3661 aacggtgttt cgcggacgtt ttcgaccaaa aaacaaccaa aaccatagca ttcatttaat
     3721 aaataacgac aatttatcgc tattttagga caaatactct aaaaaaaatg ccttttttaa
     3781 ggctttattc cttcggggtt tcgcggacac ttgaacggtg tttcgcggac gtttttgacc
     3841 aaaaaacagt caaaaatata gattttattt gatattaaac aggcaaatta tcgctattct
     3901 atgacatatg ctcaaataaa aatgtctttt ttttaatctt tttctttcgg tgtttcgcgg
     3961 acgtttgaac ggtgtttcgc ggacgtttga acggtgtttc gcggacgttt gaacggtgtt
     4021 tcgcggacac ttgaacggtg tttcgcggac gttttggcga tttccttctt tattgtattt
     4081 atttaacata tatttaacat actattaact tgttcagttc ttacgcgcga ggattttttg
     4141 tggataactt ttttttgcga acggcgtcaa aaccaagttt gtttgagcaa aaaaaatgga
     4201 gtcatgaatt tttttatttt tgacaaagaa cacgttatat ttttgcctct tttttttgcg
     4261 tttagcagtc taacaatcaa aaaaaccaac aaaaaacctg acaacaactt aaactaggag
     4321 caagaacaaa cacaggaagc aacaacagta acaagaacca gtagcaaaca catacaggtc
     4381 aaaagcttaa aaaaatttta gaaaagagcg tattttaaaa attgaaaaga gaaattgatg
     4441 tatttttaca agaaaaccga aacccaaaac aaaaatcaac gctataaccc ctattttaag
     4501 ccatacgagc tttaattttt tttttagtgc taaggtactg ctttgactta acgttcgtta
     4561 tacgcgctta tagaggctaa aagagctatt gacataaaag cagcaaaaca accacttggt
     4621 ctatcatgaa tttatctata ttcgaggaat atcaaaacta tgtctgcaaa caaaaaaact
     4681 gcattttttt caaataaatc tttaaatata ttaggttcgt ttagtagtgg tcgcttaaat
     4741 gcaataattg aaagatatga aaatttaatt caaattataa aaattgatga tatttttttg
     4801 gataatgagt ttcaattatt atatacagtc tgcaaagaat cagcaataaa agagagaaca
     4861 gattggcaag aatttaaaat accaagttta attgaaaaaa ttaataatca cccttgtgag
     4921 tttgaaacat cagaaatcat actaaaatta aaaaatatga atagcctaca ggaattatca
     4981 ctcatagaaa aaatagaaag atattggcta gaggaacgag ttaaactatt taatgaatat
     5041 agaaataaaa aaaatccatt gtaaattcat ttatgtataa tctccgtatc acctataagc
     5101 gatgtgttaa agcaaacaca aagaagcatt gactaacgat gagcgtacta aactgagtca
     5161 gccttaatgg tgctcactaa aaggaagcaa tggatgaacg acaagattaa ccaagatagc
     5221 atcaataaag cactgtggag tgcctgcgat acctttagag gcactatcag tgcagacacc
     5281 tataaagact tcatcttgac catgctgttt ctcaagtaca tatccgatgt atggcaagat
     5341 cactacgatg gctacaaggc cgagtacggt gacgagccag aactcatcga agaaatgatg
     5401 aaaaacgaac gctttgtact gcccaaagcg gccagctttt atgctcttta cgaacgtcgc
     5461 cacgaagctg gtaacggtga gcgcatcgac caagcattgc acgctattga agaagccaac
     5521 ggcaccaagc tgaaagacgc tggcaaaagc gtgttccaag acatcagttt taataccgac
     5581 aaactgggtg aggaaaagca aaaaaacact attttgcgtc acctgttgga agattttgcc
     5641 aagccagagc ttaatcttaa gcccagccgt gttggcacgt tggacgtgat tggtaacgcc
     5701 tacgaatatt taatcaaaaa cttcgccgcc agcggcggtc aaaaagcggg tgaattttac
     5761 accccgccag aagtcagtga cctaattgcc gagctgttag acccacaacc aggtgatagt
     5821 atctgtgacc cagcttgtgg ttctggttcg cttctcatga aatgtggtcg taaagtcgtc
     5881 gccaaccacg gcagcaagca atatgctctc tatgggcaag aagcgattgg ttcaacttgg
     5941 tcgttggcca aaatgaatat gtttttgcat ggcgaagata accataaaat tgaatggggc
     6001 gacaccatcc gcaaccctaa gctactcgat aaaaatggca acttaatgtt atttgatatc
     6061 gtaaccgcta acccgccgtt tagcctagat aaatggggtc acgacgaagc cgaacacgat
     6121 aagttcagcc gttttcgccg tggtgttccg ccaaaaacca aaggcgacta tgcgtttatc
     6181 ttgcatatga tcgaaacact aaaacccaaa agcggaagaa tgggcgttgt ggtaccgcac
     6241 ggcgtgttgt ttcgtggctc gtctgagggc aatattcggc aaaaattgat tgacgaaaat
     6301 ctactggatg cggtgattgg tctgcctgag aagctgtttt acggcacggg cattccagcg
     6361 gctatcctta tttttaagaa gaataagcgc gacaataatg tcatttttat cgacgccagt
     6421 cgcgacttta aagcgggtaa aaaccaaaac ctgctgacag aagaaaatat tgccaagatt
     6481 attgccacct atcacgcttg caacaacgtc gataaatacg cctatgttgc cagcctcgat
     6541 gaaattaaag aaaacgatta caacctcaac atccctcgtt atgtagatac ctttgaagaa
     6601 gaggaagaaa tcgacctgat ggcggttcgt gccgagcgtg aacaactaca agcgcaactg
     6661 accgagctgg aaaccgagat ggctaagtat ctggaggagt tggggtatga ataagccatc
     6721 gctacagcac tatgatcggc tgatagacga gatttctggc atattcattc aagggcaagc
     6781 cagaaccatc caagcaatca atgctggttt ggtcatgact tattggcaga ttggccaaca
     6841 tattgtggag tttgaacagc aaggacaagc cagtgctgaa tacggaacgc agttgctggc
     6901 caatataagc cgcgatctac gcttaaagca tggcaaaggt tttagccttt caaacctaaa
     6961 gcgatttcgc cagttttact tagcttatcg aaatggtgcg acagtgtcgc accaattaag
     7021 ttggtcacat tatgttgaat tactaaaaat aagcgatgaa acagagcgca gcttttacac
     7081 aaatcaagcg cagtcggcaa actggagcgt gcgtgaatta aaacgccaaa aagacacggg
     7141 gctatttttg cgccttgcca ccagtaaaaa caaaagcgaa atactggaac tggccaaccg
     7201 tcatgcgctg attactaaac ctgaagatgt tcaacgcgat agttatgttt ttgagttttt
     7261 aaatttaccc gaacgggaaa actacagcga gcgcgaacta gaaacagcac tctgtgagca
     7321 tttagagcac tttttgttgg agctaggcaa aggttttacc tttgttgggc gtcagtatcg
     7381 gattaccctt aataataccc actataaggt ggatttggtc ttttaccacc gtattttgcg
     7441 ctgttttgtg ctgattgatt tgaagattaa tgatgtaaag caccatgata ttggccaaat
     7501 gaatatgtac atgggctatt ttgccgcaga agaaaacatc gaaggcgata accctcccat
     7561 tggtattatt ttaagccgcg acaaagacga gctattggtg gaatacgcta cctatggcat
     7621 ggacaccagt ttgtttgtac aaaaatacca attgtatttg cccaatgagc aagagcttcg
     7681 ccgcgaaatt gaagctacac tggcggagca ggagaaaaac aatgaatagt gtttttaagg
     7741 cgcaactgac cgagatggct aagtatttga aggagctgga atatgttgcc taagggatgg
     7801 acgttaacaa gcttacgtga agtctgttct ggtaatttgc agactggtcc ttttggcagc
     7861 caactgcatg cccacgaata tgttccgact ggtattccag tattaatgcc caaggatctg
     7921 attgactgtc gtgctgactt gattggtgcc gcaaaaatca cacaagcaaa agccaattct
     7981 cttggcaaac atgtattgaa agttggggat ctacttttca gtcgtagagg tgatgtttcc
     8041 cgctttgctc tcattgattc tgattcggag ggggctctat gtggtacagg atgtttgaag
     8101 gcgaggccaa attctcagca ctcctcaaag tttcttgcat actttattca aaaagaaagc
     8161 gttaaaaaat ggctagagca aaatgctgtt ggccagacta tgccaaatat gaacacggag
     8221 attttgggtg agctcccatt aattgcggca tcatcaaaag aagaagagga aaaaatcgcc
     8281 caaatcctct ccacttggga caaggccatc accaccaccg agcaactgct cgccaacagc
     8341 cagcagcaaa aaaaagccct gatgcaacaa ctgctgacag gcaaaaaacg cttgctggat
     8401 aacaatgggg ttaggtttgg gggggagtgg aaacgggtcg agttaggaga cttactggat
     8461 tatcaacagc cgactcctta tttggttgag agcactgaat acagtgatga cttttcaaca
     8521 cctgttctta cggcgggtaa gacgttcatt ctcggatata ccgatgaaga atcgggcata
     8581 ttttcggaaa agctgcctgc tataattttc gatgatttta ctacagatag taaattcgtg
     8641 gatttccctt ttaaagcaaa atccagtgct atgaagattc tgaccgccaa aaaaggtgtt
     8701 tcgataaaat atgtatatga ggcaatgcag ttgctagatt tcgctgttgg agggcatcag
     8761 cgtcactgga tttcaatcta ttcacgtctt gttattccat ttcctgcaac agacgaacaa
     8821 caaaaaattg ccgccatttt atctaccgcc gatcaggaaa tcaccgctct gcaacaaaag
     8881 ctggatgccc tgaaacaaga gaaaaaagcc ctgatgcagc agttgttgac gggtaaacga
     8941 cgtgtacaat tgaacgatca aaactctaag gctatcactt atggaaatta aacaatttcg
     9001 tcttgcaaat ttggggcgtt ttagcgagct agaagtgaag ctcgcaccaa cacccgagtt
     9061 aaaaagcaat gtgacagttt ttattggaaa taatggtgca ggtaaaacgt cgatattaaa
     9121 agcattggca accagtctaa gttggtttgt tgcgcggctt cgcagcgaaa aaggcagcgg
     9181 cagccccatt cctgagggag tgattttaaa tggccaagcc tcggcattga tagaagtcac
     9241 agtatttgat gaccataaac agtcaaacca ttcagattca aagattgaaa aggcgatgta
     9301 tcagtggtcg ttagcaaaaa cgcaaacgag caaaaaagct aaattcaact ctaacctaaa
     9361 cgatgccagc cgtttggccg atttttacag aacggctctt acccaaaatg aaaagctagg
     9421 catgccactt atcgcttttt accctgtaga gcgcgttgtt ttagatgtgc cattaaaaat
     9481 taaagagcgt catagttttc ttcaaattga tggctatgaa aactccttaa atcagggagt
     9541 cgattttaga cgcttttttg agtggtttcg tgaacgagaa gactcagaaa atgaatctgg
     9601 gttgccacaa gatgtgatgg ataagctatt agaaacgctc ggtgacaata acgagctttg
     9661 gcataagttg cagtctttaa tggcctcttc tcgtgatagg caattaacag cagtacgcac
     9721 ggctattagc aattttatgc cgggttttag ccatttaaga gttcgccgta agccacgttt
     9781 acatatgtcg attgataaaa atggtcaaac gcttaatgtg ttacagcttt cacaaggcga
     9841 aaaatcgttg atggcgttag tgggtgatat tgctcgccgt ttagccatga tgaatcccgc
     9901 gctagaaaac ccgctgcatg gtgatggtgt tgtgcttatt gatgaagtgg atatgcacct
     9961 acaccctact tggcaacgca gtattattga gcgactaaca tcgaccttcc ccaactgcca
    10021 atttatttta agcactcact cgcctttggt gattagtgat tataaagatg tattggttta
    10081 ttcattagac gacggcgagg tcagtcaggt gccatcgcaa tatggggagg atgcaaatac
    10141 ggtgttgctg gatgtgatga atacgaatat ccgcaatgcc gtgatcaatg tgcgaattaa
    10201 tgatctgcgt gatctgattg aagacggcag gctgaacgaa gcacaagctt tgctgcatga
    10261 actggaagga gagctgcccg cgaaaaatat ggagctggct aaggcacggt tgctgctgcg
    10321 caaggaggag ctgcgccatg cgaaagatta agaaaggcat agaacctgcc agccttacta
    10381 catttaaaag aaatatgccg catgctcgct atccagattt aaccgtatta gagcggcagg
    10441 atattcggca agcctgcacc gaagaacagt tttatttatg tgcgtattgt tgccaagcga
    10501 tttctggtag cgaccaagat acgatgaacg agcatgtgga agcacagcaa ttagcaccca
    10561 atcgcacatt agattatatg aacgtggttg cgagctgcac aacgaaacag caatgcgatg
    10621 ccgcacatgg ctctcaacca ttgccgctta ctccgctgat gaacgagtgc gaaaccgagc
    10681 tgcgcttcat gttaagtggg cgggtgcaag gcttatcaga ccgtgcccgc gaagccatca
    10741 gagtactgaa tcttggtgat acagagcaca acaacaaagc gctgattgaa aaacgcaagc
    10801 aactagtaga cggtctgatc tgggccaatt atggctccga ccctgctcga cttcagcttg
    10861 aagaagatga ggaactgatc caaatgttga tcgatgatct ggaattgccc aaaaacggca
    10921 agctggagcc gtttgcacct gttcttagca atattttgcg taaccgcctt gcggcatagt
    10981 atctaagttg aagcgccagt agttcggttt taccgaacta ctggcatgta agtcacattc
    11041 cggacaccga acttctctat gaaccagccg tacacaccca aatttcagga agaatacagt
    11101 gccaagttac cagcactgac gttattgacc aacctagggt ggtcattcct ctccccccaa
    11161 accgccttag ccgctcgtgg tggtaaagcc gatgaagtgg ttttgcgcca agtgctgcgc
    11221 agtgagcttc aaaagcgcac ttttaccttt gctggtagga gctatccact gtcagaaaag
    11281 gctatcgaca acttgattgc tgaggtatgc agccctgcac taaacgaagg cttgttagcg
    11341 gccaacgaac gcctgtacaa ccacctgttg tatggaattt cggtcactga gtttgtagat
    11401 ggcaaaaagg ccagcccgac ggttgcgctg attgactggc agaacccaag caacaacagc
    11461 tttgtcttta ccgaagaatt taccgtcacc cgttctggtg gtgttgaaag tcgccgtccc
    11521 gacattgtgt gctttgtaaa cggtattccc ctagtgataa ttgaggcgaa gcgtcctgac
    11581 ggcaatgcca ataaaggccc gaccattgac gaaggtattt cgcaaagcct gcgtaatcag
    11641 cgtcatgatg aaatcccgtt attgtttgct tatagccaaa tgttgttatc tataaacggc
    11701 aacgagggtc gttatggtac ctgcggcacg cctgctaaat tttgggctgc ttggcgtgaa
    11761 gaagacatca ctgatgccga gatgtacgcc atcaaaaaca aaacgctcaa aggtgaacaa
    11821 ctggcgaact tattcagcca ccgctcggta aaagatatca attggtacca gagcctgacc
    11881 gctgcgggag aacttgctgt tacgggacag gatcaactgt tgattagtct attaagccct
    11941 gcccgtctgc tagagatgac gcgttacttc accttgttcg ataaaaaggc gggcaaaatt
    12001 gtcgctcgtt atcagcaagt atttggtatt aagcgactga ttgatcgcat caatactcga
    12061 agtgtcactg gtggtcgtga aggtggtgtg atttggcaca ccacagggtc aggtaagtca
    12121 tttactatgg tgtttttaag caaggcactg attctgcacg aaagcctcaa acaatgccgt
    12181 attgtggaga gtgaaccaaa acgggggctg gaattatgtt gaaacattca cttcgcctgt
//
LOCUS       DAOQMB010000028        12174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2000_length_12174_cov_13.554336, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000028 DAOQMB010000000
VERSION     DAOQMB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2000_length_12174_cov_13.554336"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..455)
                     /locus_tag="PLY05_02180"
     CDS             complement(24..455)
                     /locus_tag="PLY05_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNC02139.1"
                     /translation="MSEAVNITTLDIKRSTLTEQAVDTIEDSPRQFANFAEFYPFYLS
                     QHENKVCRRLHFVGSTLGLVGLTASVITGKKRYIAAGIVAGYGCAWVGHFFFEKNKPA
                     TFTYPVYSFMGDWVMYAQSLGIAPRLARFVKSKELQAILSE"
     gene            636..752
                     /locus_tag="PLY05_02185"
     CDS             636..752
                     /locus_tag="PLY05_02185"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HNC02140.1"
                     /translation="MRLFALCLSLCLLVACGQKGALYLPEKKPPTTSPHSQS"
     gene            767..2014
                     /gene="lysA"
                     /locus_tag="PLY05_02190"
     CDS             767..2014
                     /gene="lysA"
                     /locus_tag="PLY05_02190"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004863966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNC02141.1"
                     /translation="MSVFSYRDGHLYAEQVAVNQLAQQFGTPLYIYSRAALENHYRAF
                     DEAFATVPHQVCYAVKANSNLAVLNVLARLGAGFDIVSGGELARVLAAGGDASKVVFS
                     GLGKQEAEIETALNVGIACFNVESAAEIHRINAVAVRLNKKARISLRVNPDVDAQTHP
                     YISTGLKENKFGIAIEDALGVYLEAAQLEGLEVIGIDCHIGSQLTTTTPFADALDRVL
                     AMIDTLATHNIHIEHLDIGGGLGVRYRDETPPSPAEYAQALLPKLAGRNLTVYLEPGR
                     AIAANAGILVTQVEFLKPTAHKNFAIIDAAMNDLIRPSLYGSWMDIIPVQINPNTENK
                     VYDIVGAVCETGDFLGKDRDLAINAGDLLAVCGAGAYGFVMSSNYNTRGRAAEVMVDG
                     DQAYLIRRRETINELFALESVLP"
     gene            2154..2990
                     /gene="dapF"
                     /locus_tag="PLY05_02195"
     CDS             2154..2990
                     /gene="dapF"
                     /locus_tag="PLY05_02195"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNC02142.1"
                     /translation="MEFTKMHGLGNDFVVIDCITQRAHLEPHQIKQIADRHFGVGFDQ
                     LLLVEAPTRPDVDFRYRIFNADGSEVQQCGNGARCFARFVRDRKLSRKNKLIVETASG
                     IIELNITANGWVRVNMGIPRLEPADIPFIAEQRALTYPLQVGEQQVTISAVSMGNPHA
                     VILVDNVNTAPVREWGRIIESHPRFPERVNVGFFQIIDRQNARLRVFERGTGETLACG
                     TGTCAAVVAGIQQGLLDERVRVQLRGGELDIRWQGEGHPVWMTGPTARVFDGILRMYE
                     WN"
     gene            2992..3912
                     /gene="xerC"
                     /locus_tag="PLY05_02200"
     CDS             2992..3912
                     /gene="xerC"
                     /locus_tag="PLY05_02200"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HNC02143.1"
                     /translation="MPLTQLQQDIDNWLDILSSQRYASDHTLAAYQRDLNKLAQFLTR
                     ENCHDWAELDNDRLTRFIGPLLSTNSLLPISVQRMLSAIRGFYDWLVQQHRLSYNPAK
                     AFKIKRQHRELPKVMDVDLVNQLLDAPAPDAPHAAKLWLRDKAIMELLYSSGLRVSEL
                     SNCLLTDIDLSSALITVLGKGKKARVLPVGRKAISAIKDWLKIRSDFIKAETGNHIFL
                     NERGGRFTERGIQQALAYQAQRVGIPQHLHPHLLRHCFASHLLESSQDLRAVQELLGH
                     ASISTTQIYTHLDYQHLAQVYDQSHPRAKK"
     gene            3912..4619
                     /locus_tag="PLY05_02205"
     CDS             3912..4619
                     /locus_tag="PLY05_02205"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC02144.1"
                     /translation="MIKLLTFDLDNTLWSVDPIIARAENAMMEWIYQNHPELRFKVTT
                     VQFEQYKDKISSERPDISHDLTQLRLTTLRQVLDGHVTGDVNAIVKKAYEVFQRERNI
                     VQYFPYVLETLEELADQYPLIALSNGNADVHKVGIGHLFKNHFSAAGVGFAKPHPAMF
                     QYALQAIKVSAHEAIHIGDHPVQDIQAAKDVGMKTIWVNWMNHAWPLACKPDATVNNF
                     EDLPDAIAKLCQVKENA"
     gene            4619..5074
                     /locus_tag="PLY05_02210"
     CDS             4619..5074
                     /locus_tag="PLY05_02210"
                     /inference="COORDINATES: protein motif:HMM:NF001095.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HNC02145.1"
                     /translation="MQIWVDADACPVVVKEILFKAAERLKVSLTLVANQHIRVPASQV
                     IRSIQVPSGFDVADNWIAEQVQNKDLVITADVPLAAAVVAKGAFALNPRGEFYTPDTI
                     SNRLTMRNLMTELRETGQIQSYTSGYSAKDKQNFANQLDRFLSKHIKVC"
     gene            complement(5117..5668)
                     /gene="orn"
                     /locus_tag="PLY05_02215"
     CDS             complement(5117..5668)
                     /gene="orn"
                     /locus_tag="PLY05_02215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HNC02146.1"
                     /translation="MAINKETNLIWIDLEMTGLNPDTDRIIEIATIVTDANLNILAEG
                     PMLAIHQKDIVLNAMDEWNTRQHGQSGLTERVRRSTITEAEAEAQTIEFLSRFVDAKK
                     SPMCGNSICQDRRFLYRTMPKLEAFFHYRNLDVSTVKELARRWRPEIMQGFSKQSSHL
                     ALNDIRDSIDELRYYREYFFRVQ"
     gene            5760..6764
                     /gene="rsgA"
                     /locus_tag="PLY05_02220"
     CDS             5760..6764
                     /gene="rsgA"
                     /locus_tag="PLY05_02220"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HNC02147.1"
                     /translation="MSKRHLSEQQTKRIHQQHARRIAKAKEPVIDDSHLSPPQQGLII
                     AHYGRQIEVKGLEGEFVNQIWRCHIRSNLEALVTGDLVVWQADTTNQIGVITALLPRT
                     SLLTRPDPYQKIKPVAANISLILIVFAPIPVPSASLLDRYLVACETVGIKPLLVLNKS
                     DLLNNEEGQLLRDLLAEYQHLGYDSIEVSCFGNLHELQQRIGNHNVVFVGQSGVGKSS
                     LINALLPNAQQKVNIISENSLLGQHTTTTTRLFALENGGALIDSPGIREFGIWHLDEE
                     QIKQGFTEFKDFYGTCRFRNCQHLSEPDCALKQAVADGKISERRLESLHRLLAESKSN
                     "
     gene            6865..7278
                     /locus_tag="PLY05_02225"
     CDS             6865..7278
                     /locus_tag="PLY05_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC02148.1"
                     /translation="MEHFVQFLMNHYILSSTFLGLLIAFFIVESKRGGQNVSPQGLSQ
                     MINKQNALVIDLRDPTEFKKGHITGSQNIPYAKLNDKNNHFPKDRPIVFVCNLGQVAG
                     AAAKQIKTEHQLNDVFKLDGGISNWKALSLPLVKK"
     gene            7283..7540
                     /gene="grxC"
                     /locus_tag="PLY05_02230"
     CDS             7283..7540
                     /gene="grxC"
                     /locus_tag="PLY05_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNC02149.1"
                     /translation="MMAQVVIYTTRICPYCVRAKQLLQRKGVAYEEVDVSNDHATRMQ
                     LVERTKQRTVPQIFINDQHIGGCDDLYALERQGALDPLLAQ"
     gene            7562..8029
                     /gene="secB"
                     /locus_tag="PLY05_02235"
     CDS             7562..8029
                     /gene="secB"
                     /locus_tag="PLY05_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HNC02150.1"
                     /translation="MSENQNQGGQFGLERLFVKDLSFEVPNSKVFLGEWKPEMDVQLN
                     TETVRLDETHYEVSVTVTVTAKNAGATAFVAEVKQAGIFLIAGVPDEQLTQLVGAYCP
                     NILFPYVREAISDLVNRGSFPQLLLAPVNFDALFAQAQQQRAEQAAQKQATLQ"
     gene            complement(8068..9294)
                     /locus_tag="PLY05_02240"
     CDS             complement(8068..9294)
                     /locus_tag="PLY05_02240"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNC02151.1"
                     /translation="MLNRLWPALFLLAFFSCLYQAILGHPEVLAQTIEASFKTAKTAV
                     EISIGLVGLLCFWLGLFQIAEKSGLTDRLAWLLTPLFQKLMPEVPKGHPAIGSVTLNM
                     AANMLGLDNAATPMGLKAMKDLQSLNPHPDTASNPQILFLVINTSSITLIPVSIFMYR
                     AQFHAPDPASVFLPILFATSCSTLAGILLVAWLQKLDLFNRVVLAYAAGFAVLMAGLI
                     SLVVGLPPDALSERSALIGNLALFGIIVLFLLAGWRKNVALYDEFITGAKQGFDLAVG
                     LIPFLVAMLVAVSMLRASGVLTMILDGIAYVIGLLGLNTDFVPALTTALIKPFSGSGA
                     RAMMIETMQTYGVDSFPALVAAVIQGSSETTFYVLAVYFGAVGIRKERYALACGLFCD
                     LVGAIAAISAAYWFFY"
     gene            9816..10493
                     /locus_tag="PLY05_02245"
     CDS             9816..10493
                     /locus_tag="PLY05_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HNC02152.1"
                     /translation="MMLGRLLLLCSALTVYSLPSWAGNIYKFRDADGNILFTNVVDGR
                     GKPRGQDFGRYTKLEKVTWFADTNVHKYRNWGKDEFAVRPSFSKNRDAYDDIIAQAAS
                     TYGLDKGLVKAVIHTESGFNPLARSGPGAKGLMQLMPATAKRFKVDDVYDPAQNIRGG
                     AEYLSFLLKRFNSLELALAGYNAGEGNVDKYKGIPPFAETQDYVRRVISRYNKLYGGS
                     AERLSMN"
     gene            10493..11272
                     /gene="blaOXA"
                     /locus_tag="PLY05_02250"
     CDS             10493..11272
                     /gene="blaOXA"
                     /locus_tag="PLY05_02250"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF012161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HNC02153.1"
                     /translation="MRLRWLFSAVFIGLVNVPCYAQTEIAQVFKTHQVQGTIVLRSLK
                     QDVAIIYNPDRAQLPFASASTFKILHSLIALEEKVITKTDVIKWDGTTYDINTWNQDQ
                     TLASAFQVSCVWCYQTFAKQISAKKYQHYIDSTHFGHLSPNFAQTTFWLDGSLTISAL
                     EQVAFLKKLYQHQFAFSADHYQTLAEIMLAEKTNDYRLYAKTGWAARSTPQIGWYVGY
                     VETQQDTWFFALNININDTKDLALRQLITKQVLTNQHIISP"
     gene            complement(11303..12091)
                     /locus_tag="PLY05_02255"
     CDS             complement(11303..12091)
                     /locus_tag="PLY05_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC02154.1"
                     /translation="MRRVIFNQKGGVGKSSITVNLAAVSASQGLKTLVLDLDPQCNAT
                     QYLLGEVAEVAANKPELQPNIGQFFAQTLSLKGKEKPLAEYVHTTAFNNLHVIPSNPE
                     LGEIEHLLQSKHKIYKLAAALKELSRQYDNIFIDTPPAFNFYTLSALIASERCLIPFD
                     CDEFSRRALYTLLENVGETKADHNADLEVEGVIVNQFQPRASLPLQLINELKEEGLPI
                     MATYLPSSVIMKESHHKSLPLVFLAPQHKLTEQYVALFGELKDA"
BASE COUNT         3717 a         2454 c         2341 g         3662 t
ORIGIN      
        1 tcgcttaggc agttatcact tatttattca ctcaaaatcg cttgcagctc ttttgacttc
       61 acaaaacgtg ctaatcgcgg tgcgatgcct aagctttgcg catacatcac ccaatcaccc
      121 ataaaactat acacaggata tgtaaacgtt gctggcttat ttttttcaaa aaagaaatgc
      181 ccaacccacg cacaaccata acccgcaaca attcctgccg ctatataacg ctttttgccc
      241 gtaatcacac tcgcggttaa acccactaag cccaaggtac tgcctacaaa atgcagacga
      301 cgacagactt tgttttcatg ttggcttaaa taaaaaggat aaaattcggc aaaattggca
      361 aattgacgtg gtgaatcctc gatagtgtct acggcttgct cagtcaatgt gctgcgtttg
      421 atgtctaaag tggtgatatt tacagcttca ctcatgaagt cttactcctg ttgtttttga
      481 actgcatcat cagttattga ctcactaaaa aaattctaac gatttttagc cgtcaaaatg
      541 cactaatttc agagtcctgt ttgtctagct agttttatta tcaaatcgat tccgttagca
      601 tagcgcatct ttttattgat accacttttt ttattatgcg cctattcgct ttgtgtttga
      661 gtttgtgcct tttagtcgct tgtggtcaaa aaggggcttt gtacctacct gagaaaaaac
      721 cacccacgac ttctcctcat tcccaatctt aagagtttat ctaaacatgt cagtttttag
      781 ttatcgtgac ggccatttat atgccgaaca agttgctgta aatcaattag cccaacaatt
      841 tggcacacca ctgtatattt attcacgtgc agccttagaa aaccattacc gtgcttttga
      901 tgaagccttt gccactgtgc cccatcaagt gtgttatgcc gttaaagcta actccaactt
      961 agccgtactc aatgttttgg cacgcttagg cgcaggtttt gacatcgtat ctggtggtga
     1021 attagcccgt gttttagcgg ctggtggtga cgcaagcaaa gttgtttttt cgggtttagg
     1081 caaacaagaa gccgaaatcg aaacagcatt gaatgtcggt attgcttgtt ttaatgtcga
     1141 atctgcggcc gaaattcatc ggattaatgc cgttgcagta cgtttaaaca aaaaagcacg
     1201 tatttcttta cgtgttaatc ctgatgttga tgcccaaacc catccttata tttcaacagg
     1261 tttaaaagaa aataaatttg gcattgccat tgaagatgcc ttgggtgtgt atttagaagc
     1321 cgcgcaatta gaaggcttag aggttattgg tattgattgt catattggct ctcaattaac
     1381 cacaacaacg ccatttgccg acgctttaga tcgcgtattg gcgatgatcg acactttagc
     1441 cactcacaat attcatatcg aacatttgga tattggcggt ggtttagggg tacgttatcg
     1501 tgatgaaaca ccacccagcc ctgccgaata tgctcaagct ttattaccta aattagcagg
     1561 ccgcaattta actgtttatt tagaaccagg tagagcgatt gctgctaatg caggtatttt
     1621 ggtcacacaa gttgagtttt taaagcccac tgctcacaaa aactttgcca ttattgatgc
     1681 cgccatgaac gatttaattc gtccgtcatt atatggctca tggatggata ttattcctgt
     1741 acaaattaac ccaaacaccg aaaacaaagt gtatgacatc gttggtgcag tttgcgaaac
     1801 aggtgacttt ttggggaaag accgtgattt agcgattaat gcaggcgatt tattagccgt
     1861 ttgtggtgca ggtgcttatg gttttgtcat gagttccaac tacaacaccc gtggtcgtgc
     1921 agccgaagtg atggttgatg gcgatcaagc ttatttaatt cgtcgtcgtg aaacaattaa
     1981 tgagttattt gcgctcgaat ctgtgctacc ttaagcttaa agttttttag aataatcaag
     2041 acttaggcgt tttatttact cactccagcc ctttcccacc agagagggtg tcttctatta
     2101 gagatagtga gcaataaata cctaagttgt agaaattgga gagcaatatg cgtctggaat
     2161 ttaccaagat gcatggcctc ggcaatgatt ttgttgtcat cgattgtatt acgcagcgcg
     2221 cacatttaga gccacatcaa atcaaacaaa ttgccgatcg ccattttgga gtaggttttg
     2281 accaactatt attggttgaa gcccccactc gtcctgacgt ggactttcgc tatcgcattt
     2341 ttaatgccga tggctcagaa gtacaacaat gtggcaacgg tgcacgctgt tttgcccgtt
     2401 ttgttcgtga tcgcaaactc agtcgcaaaa acaaactgat tgttgaaacc gccagcggca
     2461 ttattgaatt aaacatcacc gcaaatggtt gggtgcgggt taatatgggt attccacgct
     2521 tagagcctgc cgatattccg tttattgctg aacaacgcgc tttgacttac ccattacaag
     2581 ttggcgaaca acaagtcact attagtgcag ttagcatggg caatcctcat gcagtgattt
     2641 tggttgataa tgttaatacc gcacctgtgc gtgaatgggg acgaattatc gaaagccatc
     2701 cacgttttcc tgaacgtgtg aatgttggct tctttcaaat tattgatcgg caaaatgctc
     2761 gtttacgggt gtttgaacgt ggtacaggtg aaaccttagc ttgtggcaca ggcacttgtg
     2821 cggctgttgt tgccggtatc caacaaggtt tattggatga gcgtgtgcgt gttcagttac
     2881 gtggcggtga attagacatt cgttggcaag gcgaaggcca tcctgtatgg atgactggcc
     2941 ctactgcccg cgtgtttgat ggcatattac gcatgtatga gtggaactaa gatgccctta
     3001 acgcagctac aacaagatat agacaactgg ctcgacatct taagtagtca gcgttatgcc
     3061 tccgaccaca ccttagccgc ttaccaacgt gacctaaaca agcttgccca gtttttgact
     3121 cgcgaaaatt gccatgattg ggcagagtta gacaacgatc gcttaacgcg ttttattggg
     3181 cctttattgt ctactaacag tttgttacca attagtgtgc agcggatgtt atcggccatt
     3241 cgtgggtttt atgactggtt agtgcaacaa catcgactaa gttataaccc tgcaaaagcc
     3301 tttaagatca aacgtcaaca ccgtgaatta cctaaggtta tggatgttga ccttgtcaat
     3361 cagctcttag atgcccctgc accagatgcc cctcatgcgg ccaaattatg gctgcgtgac
     3421 aaggctatta tggagttact ctactctagt ggccttcgtg tttctgaatt aagcaattgt
     3481 ttattaactg atattgattt aagctcagca ttgatcaccg ttttaggtaa aggcaaaaaa
     3541 gcgcgtgtgt tacctgtagg acgtaaagca ataagtgcta ttaaagattg gctcaaaatt
     3601 cgcagtgatt ttattaaagc agaaacaggc aaccatatat ttttaaatga acgtggtgga
     3661 cgttttaccg aacgtggcat tcaacaagct ttagcctatc aagcgcaacg ggttggtata
     3721 ccacaacatc tccatccgca tttattaaga cactgttttg cctcacatct gctagaatct
     3781 agtcaagact tacgcgctgt acaggagtta ttgggtcacg cgagtattag cactacccaa
     3841 atttatactc atttggatta tcagcatctt gcgcaggttt atgatcaaag ccatccaaga
     3901 gcgaaaaaat aatgataaaa cttctgacat ttgatttaga caatacctta tggtctgttg
     3961 accctattat tgctcgtgcc gaaaatgcaa tgatggaatg gatttatcaa aatcatcccg
     4021 aattgcgttt taaagtcacc actgttcagt ttgaacagta taaagacaaa attagtagtg
     4081 aacgccccga tattagccac gatttaaccc aactacgttt aaccacttta cgtcaagttt
     4141 tagatggtca cgtgacaggt gatgtaaatg ctatcgtcaa aaaagcttat gaagtatttc
     4201 aacgcgagcg caatattgtc caatatttcc cttatgtgct tgaaacactc gaagaacttg
     4261 ccgatcaata tccactaatt gctttaagta atggtaatgc tgatgtccat aaagttggca
     4321 ttggccattt gttcaaaaat cacttttctg ctgcgggggt tggctttgct aaaccgcatc
     4381 cagccatgtt tcaatatgct ttacaagcaa taaaagtgag tgcacatgaa gccattcata
     4441 ttggcgatca tcctgtacaa gatattcaag cggctaaaga tgtaggcatg aaaaccattt
     4501 gggtaaactg gatgaatcat gcttggccgt tagcctgtaa acctgatgca accgtcaata
     4561 attttgaaga cttacctgat gccattgcta aactttgtca agtaaaggaa aacgcctaat
     4621 gcaaatttgg gttgatgccg atgcctgccc cgttgttgtt aaagaaattt tatttaaagc
     4681 ggctgaacga ctcaaagtgt cactcacttt ggtcgccaat caacatattc gtgtccctgc
     4741 ctcgcaagtg attcgctcca tacaagtccc atctggcttt gatgttgcag ataattggat
     4801 tgccgaacaa gtgcaaaaca aagatttagt gattaccgca gatgtgcctt tagcggctgc
     4861 tgtggttgct aaaggtgctt ttgcacttaa tccgcgtggc gaattttata cacctgatac
     4921 catttctaac cgtttaacca tgcgcaattt gatgacggaa ttacgtgaga ctggccaaat
     4981 tcaaagttat acttcaggat atagcgcgaa agataagcaa aattttgcta atcaattaga
     5041 tcggttttta agcaagcata ttaaagtctg ttgacgtttt caacagactt tagtttagga
     5101 cttacgcggt taaagtttat tgcactctaa aaaaatactc acggtaataa cgtaattcat
     5161 caatcgaatc acgaatatca tttaacgcta aatggctact ttgtttagaa aatccttgca
     5221 taatttcggg acgccaacga cgagctaatt ctttaacggt gctaacatcc aaattacgat
     5281 aatgaaaaaa ggcttctaat ttgggcatgg tacgatataa aaaacgacga tcttggcaaa
     5341 tggaattacc acacataggc gattttttgg catcaacaaa acggcttaaa aactcaatgg
     5401 tttgcgcttc agcttcagct tcagtaatcg tgctacggcg cacacgctca gttaacccag
     5461 attgaccatg ttgacgagta ttccattcat ccattgcatt taacacaata tctttttgat
     5521 gaatggctaa catggggcct tcagccaaaa tatttaaatt tgcatcagtc acaatggtgg
     5581 caatttcaat aatacgatcg gtatctgggt ttaaaccggt catttctaaa tcaatccaaa
     5641 ttaaattggt ttctttgtta atagccattt tcgtcctagt ttattagtca caagaaaaat
     5701 gactattcta cagaaaaaat atatgatagg cgtttttatt tcgttgtttt aaagtgccta
     5761 tgagcaaacg ccatttaagc gagcagcaga ctaaacgtat tcatcaacaa catgcgcgtc
     5821 gtattgctaa agctaaagaa cccgtgattg atgacagtca cttaagccct cctcaacaag
     5881 gattaattat tgcccattat ggccgtcaaa ttgaggtaaa aggattagaa ggcgaatttg
     5941 ttaatcaaat ttggcgttgt catattcgta gtaatctaga ggcgttagtg acaggcgatt
     6001 tagtggtttg gcaagccgat acaaccaatc aaattggcgt gattactgca ttattgccca
     6061 gaacttcgtt gctcacacgt cctgatcctt atcaaaaaat taagccagtt gccgctaata
     6121 tctccctaat tttgattgtt tttgcaccaa ttccagttcc ttctgccagt ttgttagacc
     6181 gttatttagt tgcttgtgaa acggttggca ttaaacctct gttagtctta aataaatcag
     6241 atttactaaa taacgaagaa ggtcaattac ttcgtgactt attagccgaa tatcaacatt
     6301 taggctatga cagtatagaa gtgtcttgtt ttggcaattt gcatgaatta caacaacgaa
     6361 ttggcaatca taatgtcgtt tttgttggcc aatctggtgt gggtaaaagc tccttaatta
     6421 atgccttgtt acccaatgca caacaaaagg tgaatattat ttctgaaaat tctttattag
     6481 gccaacatac cacaactact actcgcctat ttgccttaga aaatggtggt gctttaattg
     6541 actcaccggg tattcgtgag tttggtattt ggcatttaga tgaggagcaa attaaacaag
     6601 gctttactga gtttaaagat ttttatggca cttgccgttt tagaaattgc caacatttaa
     6661 gtgagcctga ctgcgcactt aaacaagcgg ttgctgatgg taaaatatct gaacgtcgct
     6721 tagaaagtct gcaccgtttg ttggccgaaa gtaaatctaa ctagagacaa agtttcaagc
     6781 aagttatact ccgccccgtt tttatgatta tgctgtgaat tagttatcta gcagactaat
     6841 ttgcctttat gaggtctaca tgtaatggaa cattttgttc aatttttaat gaatcactac
     6901 atactcagca gtacgttttt agggctgttg attgcgtttt ttattgttga aagtaagcgc
     6961 ggtggtcaaa atgtcagccc tcaaggctta agtcagatga taaataaaca aaatgccctt
     7021 gttattgact tacgtgaccc cactgagttt aagaagggac acattactgg cagtcaaaat
     7081 ataccttatg ccaaattaaa tgacaaaaac aatcattttc ctaaagatag accgattgta
     7141 tttgtctgta atttaggtca agtagcaggt gctgctgcca aacaaatcaa aacagagcat
     7201 caactcaatg atgtatttaa attggatggt ggcatctcta actggaaggc gttgtcatta
     7261 cctttagtaa aaaagtgaga agatgatggc acaggttgtt atttatacaa cacgaatttg
     7321 tccttattgc gtacgtgcaa aacaactact gcaacgcaaa ggtgttgctt atgaagaagt
     7381 tgatgttagc aatgaccatg cgacacggat gcaattagtt gagcgcacca aacaacgtac
     7441 tgtgccacag atttttatta atgaccaaca cattggtggt tgtgatgatt tatacgcgtt
     7501 agagcggcaa ggcgcgttag accctttact tgcccagtaa aacataaact aggaaataac
     7561 tatgagcgag aatcaaaatc aaggtggaca atttggctta gagcgtttgt ttgtcaaaga
     7621 tttatctttt gaagttccaa actccaaagt atttttgggc gaatggaaac cagaaatgga
     7681 cgtgcaatta aatactgaaa ctgtacgttt agatgaaaca cattacgaag tttctgtgac
     7741 tgtgactgtg accgccaaaa atgcaggtgc tactgctttt gttgctgaag tgaaacaagc
     7801 aggtattttc ttaattgctg gtgtacctga cgagcaatta actcaattag ttggcgcata
     7861 ctgtcctaac attttgttcc catacgttcg tgaagccatt tctgatttag tcaatcgtgg
     7921 tagcttccct caattattgt tagcacctgt taactttgat gctttatttg ctcaagcaca
     7981 acaacaacgt gccgagcaag cagcacaaaa gcaagctact ttacagtaaa cttgttaaat
     8041 ggctgtcact cttgagtgac agctatttta ataaaaaaac caatacgccg cgctaatcgc
     8101 tgcaatcgct ccgactaaat cacaaaacaa gccacacgct aaagcataac gttctttacg
     8161 aatgccaact gcgccaaaat ataccgctaa cacataaaaa gtcgtttcgc ttgagccttg
     8221 aataaccgcc gcaactagag ctggaaacga gtccacacca taggtttgca tggtttcaat
     8281 catcatcgca cgcgcaccac tccccgaaaa aggcttaatg agtgccgtgg ttaaagctgg
     8341 cacaaaatcg gtatttaagc ccaataaacc aatgacataa gctatcccat ctaaaatcat
     8401 ggtcaaaaca ccactggcac gcaacattga caccgccacc aacatcgcca ccaaaaaggg
     8461 aattaatcca acagccaaat caaaaccttg ttttgcgcca gtaataaact cgtcatacaa
     8521 cgcaacattt ttacgccaac ctgccaataa aaataaaacg ataataccaa acaaggctaa
     8581 attaccaatt aacgctgagc gttcacttaa agcatctggt ggcaaaccca ccactaaact
     8641 aattaaacca gccattaata cggcaaaacc agccgcataa gccaatacca cacgattaaa
     8701 taaatcgagt ttttgtaacc aagcaactaa taaaataccc gctaaggtag aacaacttgt
     8761 ggcaaataaa atgggcaaaa aaacactggc aggatcaggt gcatgaaact gcgcacggta
     8821 cataaaaata ctgactggaa ttaaggtaat cgacgaggta ttaatcacta aaaataaaat
     8881 ttgtggatta ctggccgtat cgggatgcgg attaagactt tgtaaatctt tcatcgcttt
     8941 taaacccata ggcgttgcag cattatctaa ccccaacata tttgctgcca tatttaaagt
     9001 cactgaacca atagcaggat gtcctttggg aacttcgggc attaattttt gaaataacgg
     9061 cgttaataac cacgccaaac gatcagttaa accactcttt tcggcaattt gaaataaacc
     9121 caaccaaaaa cataacaaac caactaaacc aatagaaatt tctactgccg ttttagctgt
     9181 tttaaaactg gcttcaatgg tttgagctaa aacttcggga tgtccaagta tggcttgata
     9241 taaacaacta aaaaatgcca gcaaaaataa tgcaggccac aatcgattca acataagatt
     9301 tcacgtccat ccaagaaagt ctgtgagaca atctttttta aaagtatttg tcaatatttt
     9361 ttgcctataa accaaacaaa aaatcacttt ttgatgcttt atgtacagat aagcatcaaa
     9421 ctgcctaaaa gaccaacaat aaaaagttgt ccacaaaatc tgtggataac tctgtggata
     9481 actctaggtt accccctgtt caagctagtg ttatcaaggc ttagagttaa atcgtatatt
     9541 ttttaaccta aacaaaaaat cattttataa tcaataactt aatttttaca atactgtaac
     9601 aatcaatttt tataaaaaaa tcacttgctt atattatgtc aatactgcaa caaaaactat
     9661 tgattatttt ttaatcattt gatcagaaaa aatgtttcta aaattattac caaatgcaca
     9721 ctttttaaac aaatatttgt tgacagaaaa aacttgtcta agctcaataa agtagcgaaa
     9781 atagctcatc aatctaatca agtttaagaa taaaaatgat gcttggacgt ttattattac
     9841 tctgtagtgc gctcactgtt tatagcctac ctagctgggc tggtaatatt tataaattcc
     9901 gtgatgcgga cggtaatatt ttatttacca atgttgtcga tggccgaggt aaaccgcgtg
     9961 gccaagattt tggccgttat accaaactgg aaaaagtcac atggtttgca gataccaatg
    10021 ttcataaata ccgtaactgg ggtaaagatg aatttgctgt acgcccaagt tttagcaaaa
    10081 atcgtgacgc ttatgacgat attattgctc aagccgcttc tacttatggc ctcgataaag
    10141 gtttagttaa agccgtgatt cacaccgagt cgggttttaa tccccttgct cgctcagggc
    10201 cgggcgctaa aggtttgatg cagttaatgc ccgctactgc caaacgtttt aaagtcgatg
    10261 atgtgtatga ccctgcccaa aatattcgtg gtggtgcaga atatttaagt tttttgttaa
    10321 aacgttttaa tagtttagaa ctggctttag caggttataa cgcgggcgaa ggcaatgttg
    10381 acaaatataa aggcattcca ccctttgccg aaacccaaga ttatgtgcgc cgagtgatta
    10441 gccgttataa caaattatat ggcggtagtg ccgaacgttt gagtatgaat tgatgcggct
    10501 tcgatggtta ttttctgcgg tttttatagg cttggtaaat gtgccttgtt atgctcaaac
    10561 agagattgct caagttttta aaacgcatca ggtacaaggc acgatagtat tacgctcttt
    10621 aaaacaagac gtggcgatta tttataaccc tgatcgcgca caactcccct ttgccagtgc
    10681 gtcaacgttt aaaattttac acagcttaat tgcactagaa gaaaaagtta tcaccaaaac
    10741 ggatgttatt aaatgggatg gcacaactta tgacattaat acatggaatc aagatcaaac
    10801 cctagctagt gcttttcaag tgtcttgtgt gtggtgttat caaacgtttg ccaaacaaat
    10861 cagtgccaaa aaatatcagc attatattga tagtactcat tttggccatt tgtcgcccaa
    10921 ttttgcacaa actacatttt ggctagatgg ctctttaaca atcagtgcct tagagcaagt
    10981 tgctttttta aaaaaacttt atcagcatca atttgcattt agtgccgatc attatcagac
    11041 tttagcagag attatgcttg ccgaaaaaac caatgattat cgtctttatg ccaaaacagg
    11101 ttgggcagca cgtagcacac cacaaattgg ttggtatgtg ggttatgtag aaactcagca
    11161 agatacttgg ttttttgctt taaatatcaa tattaatgac actaaagact tagcacttag
    11221 acagctcatt accaaacaag ttttaactaa tcaacacatc attagccctt aatgcaaaca
    11281 cactaagggc ttagcatatc tctcacgcgt cttttaactc accaaacaat gctacatact
    11341 gttcagttaa tttatgctga ggtgctaaaa acactaaagg taaacttttg tgatgtgact
    11401 ccttcataat caccgatgac ggtaaataag tcgccataat cggcaagcct tcttctttta
    11461 actcattaat taattgtaaa ggcaaggaag cgcgtggttg aaactgatta acaattaccc
    11521 cttctacttc taagtcggca ttatggtcgg cttttgtttc accgacattt tctaataaag
    11581 tatataaagc gcgacgagaa aactcatcac aatcaaacgg aattaaacaa cgctcggaag
    11641 caatcaaggc tgataaggta taaaagttaa acgcaggtgg cgtatctata aaaatattat
    11701 cgtattgacg actcaattct tttaaagcag cggctaactt ataaatttta tgtttacttt
    11761 gtaataagtg ttcaatttca cctaattcgg gattagaagg aatgacgtgt aaattattaa
    11821 aagccgtggt atgtacatac tcagccaatg gcttttcttt gccttttaaa cttaaagttt
    11881 gggcaaaaaa ctggccaatg tttggttgca attctggttt attagccgca acttcggcaa
    11941 cttctcccaa taaatattgg gtcgcattac attgtgggtc taagtctaat actaaggttt
    12001 ttaaaccttg gctagcactg actgccgcta aattcacggt aatactcgat tttcctacgc
    12061 cacctttttg attaaaaatc acacgtctca tttttattta ccctctatta attcagtatt
    12121 ttttacgcaa ttaaaacaaa cgccgattgt aatcttaaca aaatcaacgt ctag
//
LOCUS       DAOQMB010000029        11875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2070_length_11875_cov_15.183841, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000029 DAOQMB010000000
VERSION     DAOQMB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11875
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2070_length_11875_cov_15.183841"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..2272)
                     /gene="fusA"
                     /locus_tag="PLY05_02260"
     CDS             complement(140..2272)
                     /gene="fusA"
                     /locus_tag="PLY05_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNC02155.1"
                     /translation="MARTTPLERYRNIGISAHIDAGKTTTTERILYYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWSGMAHQFPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFFRAVEQMKI
                     RLGASPVPMVVPIGAEDTFQGVVDLVTMKAIQWDEASQGMKFELQDIPAELAEVCQKW
                     RENLVESAAEASEELMDKYLEEGDLSEEEIHAAIRQRTLNGEIQPVLCGSAFKNKGVQ
                     AMLDAVIRYLPAPNDVAAIKGTLDDKNETESERHASDDEPFAALAFKIMNDKFVGSLT
                     FVRVYSGVIKSGDAVWNPIKQRKERVGRILQMHANQREELTEVRAGDIAAFVGMKDVI
                     TGETLCDENNIITLERMEFPEPVISLAVEPKTKADQEKMSTALGRLAKEDPSFRVKTD
                     EESGQTIISGMGELHLEIIVDRMKREFGVEANIGKPQVAYRETIRKTVETEGKFVRQT
                     GGRGKYGHVLLRLEPMGEDRDKDYEYAVEVVGGTVPKEFFGAVDKGIQERMANGILAG
                     YPVVGVKAVLYDGSYHDVDSDELSFKMAGSIGFRKGFLAADPVLLEPIMKVEVETPED
                     YMGDIMGDLNRRRGMVQGMDDLPGGGKAIKAEVPLSDMFGYATDMRSMSQGRATFSME
                     FSRYAETPKNVAEAIIAKFSGKGKDDDE"
     gene            complement(2327..2797)
                     /gene="rpsG"
                     /locus_tag="PLY05_02265"
     CDS             complement(2327..2797)
                     /gene="rpsG"
                     /locus_tag="PLY05_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNC02156.1"
                     /translation="MPRRRVVAAREILPDPKFKSQTVAKFINHVMDSGKKSVAEGIVY
                     GALDRVLEKGKADPVRFFEDTLEKVRPMVEVKARRVGGATYQVPMEVRASRRTALAMR
                     WLVDAAAKRSEKTMALRLAGELLDAYEGKGAAIKKREDVHRMAEANKAFSHYRF"
     gene            complement(2896..3270)
                     /gene="rpsL"
                     /locus_tag="PLY05_02270"
     CDS             complement(2896..3270)
                     /gene="rpsL"
                     /locus_tag="PLY05_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNC02157.1"
                     /translation="MATTNQLIRKGRTTLVEKSKVPALKACPQRRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTSGFEVSSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DCAGVKNRQQSRSKYGAKRPKK"
     gene            complement(3528..7760)
                     /gene="rpoC"
                     /locus_tag="PLY05_02275"
     CDS             complement(3528..7760)
                     /gene="rpoC"
                     /locus_tag="PLY05_02275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNC02158.1"
                     /translation="MKDLLNILQSQGKEVEFDRIRIGLASPEMIRSWSYGEVKKPETI
                     NYRTFKPEREGLFCAKIFGPIKDYECLCGKYKRMKFRGVICEKCGVEVTLTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMTLRDIERVLYFESFVVIEPGMTPLEKGQLL
                     SDEEYYTALEEYGDEFDAKMGAEAIQALLKDIDLQAEMERLREEIPNTGSETKLKKLS
                     KRLKLVESFLSSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLELSAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVIVVGPTLRLHQCGLPKKMALELFKPFIFAKLESKGLAT
                     TIKAAKKMVERETAEVWDILADVIREHPVMLNRAPTLHRLGLQAFEPVLIEGKAIQLH
                     PLVCTAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYISRDKINAKGEGMVFADFAEVTRALANKAVDIHARIKVRVREVTIDDDGNRQER
                     KFLADTTPGRCMLWNIVPEGLPYNVINQPMTKKNISKLINTCYRVLGLKDTVIFADQL
                     MYLGFRQATYSGVSVGMEDMVIPDEKQVIIGDAEEEVREIEGQYSAGLVTGQERYNKV
                     VDIWSRTNERVAKAMMDNLSTEKVINKQGVEESQKSFNSIFIMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPIKANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVITEPDCGTSQGLSMTPLIQGGDIIEPLRERVLGRVAAVDVVAPHSDEV
                     VAPAGTLLDEKWVDKLELAGVDEVIVRSVITCKTRFGVCSNCYGRDLARGHQVNIGES
                     IGVMAAQSIGEPGTQLTMRTFHIGGAANRASAISNVTVRNEGTIRFHNIKMVQHANGN
                     LVAVSRSGEIGIADSRGRERERYKLPYGAVITVKDGQEVKGGQIVANWDPHTHPIVTE
                     VAGRVKFIHMDEGITVRHQTDEQTGLTNMEIIDPKDRPASGKDLRPAIALVDAAGNYI
                     RLKDSDQPVQYFLPALAITSLQDGAEVGVGDVIGRIPQESSKTRDITGGLPRVADLFE
                     ARKPKEHAILAEKSGVVSFGKETKGKHRLVITPDDGAEPYEELIPKWRQINVFEGERV
                     ARGEVISDGPQNPHDILRLKGETALATYIVNEVQDVYRLQGVKINDKHIEVIIRQMLR
                     KVDILDGGDSSFIKGEQVEYARVVEENDVLEQNGDIPAKFERLLMGITKASLATDSFI
                     SAASFQETTRVLTEAAVTGKEDDLRGLKENVVVGRLIPAGTGFAYHAERRRQRLEALM
                     PQVKAEDNEVLNHDLEKEAAEQALLDAFSQGE"
     gene            complement(7815..>11875)
                     /gene="rpoB"
                     /locus_tag="PLY05_02280"
     CDS             complement(7815..>11875)
                     /gene="rpoB"
                     /locus_tag="PLY05_02280"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNC02159.1"
                     /translation="KNFGKLPTLMDVPHLLGIQVDSYRAFLQDGKKAKQREDVGLQAA
                     FKSVFPIQSYSGNAALEFVEYHLGHPVFDVRECILRGLTYAAPLRVKIRLVIYDREAA
                     VKTIKDIREQEVYMGEMPLMTDNGTFVINGTERVIVSQLHRSPGVFFDHDKGKTHSSG
                     KLLFNARVIPYRGSWLDFEFDAKDLVYVRIDRRRKLYATILLRGLGYNSDQILEMFFE
                     TTSIKHEDGTYKLGLIAERLRGETAAFEIASKEKVIVEVGRRITPKHIREIQKAGLTE
                     LDIPADYLHGKVIAKTLAHPATGELVCEGNTIITAEVLTRLAKAGIGQFNILYTNDID
                     RGAFIADTLRADASTRTELDALVEIYRMMRPGEPPTKDAAEALFKNLFFTQERYDLSG
                     VGRMKFNRRLNRGNDRGNGDSMEKDGVLSKQDIVDVLKTLIDIRNGKGEIDDIDHLGN
                     RRVRCVGEMTENQFRVGLVRVERAVKERLSLAESENLMPQDLINAKPVSAAIKEFFGS
                     SQLSQFMDQNNPLSEITHKRRVSALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLAVFAQSNSYGFLESPYRKVADGQVQDEIVYLSAIEESQCVVAQADSAVD
                     SEGRLLDDLVAVRHKNEFTVMAPDKVTHMDVSPRQVVSVAASLIPFLEHDDANRALMG
                     SNMQRQAVPTLRADKPLVGTGMERYVARDSGVCVTAKRGGVIDYVDAARIVIRVDESE
                     MVAGEAGVDIYNLTKYTRSNQNTCINQRTIVNRGDVVARGDTLADGPSVDLGELALGQ
                     NMRVAFMPWNGYNFEDSILLSERVVQEDRFTSIHIQELSCIARDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVNAGDILVGKVTPKGETQLSPEEKLLRAIFGEKASDVK
                     DSSLRVPSGTKGTVIDVQVFTRDGVDKDSRALAIEKQQLNAYRKDLKEEYRIFEEAAR
                     SRLLSLLKGQEVNGGAGFKKGDVLSEDNLSGLKLDQWFDIRPVDDSVADQLERVQAYL
                     QERQEEIEDKFEDKKRKITTGDDLTTGVLKVVKVYLAVKRRIQPGDKMAGRHGNKGVV
                     SVIMPVEDMPYDENGEPVDVVLNPLGVPSRMNVGQILETHLGWAAKGLGTKIGKMLDQ
                     QRQAAELREFLDKIYNQVGGEQESLEELGDNEVMALAKNLRGGVPIATPAFDGAHETE
                     IKALLRLADQSENGQQWLYDGRTGERFDRQVTVGYMYMLKLNHLVDDKMHARSTGSYS
                     LVTQQPLGGKAQFGGQRFGEMEVWALEAYGATYTLQEMLTVKSDDVNGRTRIYKNIVD
                     GDHRMEPGMPESFNVLIKEIRSLGINIELDNE"
BASE COUNT         3360 a         2870 c         2294 g         3351 t
ORIGIN      
        1 aattgtgccg acgtttacgt gcggcttatt acgttcaaac ttagccttag ccatgattta
       61 atcctcttaa atagcaggct tatgcaaaac ataagcccgt tcataacgta cttacacaag
      121 cgtgtaagtc cataaaacat tattcgtcgt cgtctttgcc tttgccagag aacttagcga
      181 taattgcttc tgctacgttc ttcggtgttt cagcataacg agagaattcc atgctgaagg
      241 tcgcacgacc ttgagacata gaacgcatgt cggttgcata accgaacatg tcagacaatg
      301 gcacttcagc tttaatggct ttgccaccgc caggtaaatc gtccatacct tgaaccatac
      361 cacgacgacg gtttaagtcg cccatgatgt cgcccatgta gtcttctggt gtttccactt
      421 cgactttcat gattggctca agtaggactg ggtcagcagc taagaagccc ttacggaaac
      481 cgatagaacc tgccattttg aacgataatt catcagagtc cacgtcatgg taagaaccgt
      541 cataaagaac agccttaaca cccacaactg gatagcctgc taaaataccg ttagccatac
      601 gctcttgaat acctttgtca acagcaccga agaattcttt tggaacagta ccaccaacaa
      661 cttcaacagc atattcgtag tctttatcac gatcttcacc cattggctct aaacgtaata
      721 aaacgtgacc atacttacca cgaccacctg tttgacgaac gaacttgcct tcagtttcaa
      781 cagttttacg aatcgtttca cgataagcaa cttgtggctt accgatgttg gcttctacac
      841 caaactcacg cttcatacgg tcaacgataa tttcaaggtg caattcaccc ataccagaaa
      901 taatggtttg accagattct tcgtcagttt taacgcggaa tgatggatct tctttcgcta
      961 aacgacctaa agcagttgac attttttctt ggtcagcttt agtttttggt tcaacagcca
     1021 aagaaataac tggctctggg aattccatac gttctaaggt aatgatgttg ttttcatcac
     1081 ataaggtttc cccagtgata acatctttca tacctacaaa cgcagcgata tcacctgcac
     1141 gtacttcggt gagttcttca cgttggttag cgtgcatttg caaaatacga ccaacacgtt
     1201 ctttacgttg tttgatcggg ttccaaaccg catcaccaga cttaatcaca ccagaataca
     1261 cgcgaacgaa ggttaaagaa cccacaaact tgtcgttcat gattttgaat gcaagtgctg
     1321 caaatggctc atcatcagac gcatgacgtt cagattctgt ttcgtttttg tcgtctaatg
     1381 tgcctttaat agcagcaacg tcatttggtg ctggtaaata acgaataacc gcatccaaca
     1441 tagcctgtac gcctttgttt ttaaaggcag agccacataa tacaggttga atttcgccat
     1501 ttaaggtacg ttgacgaatt gctgcatgaa tttcttcttc agaaagatcg ccttcttcca
     1561 aatacttatc cattaactct tcgctagctt cagcagcact ttcaactaag ttttcgcgcc
     1621 atttttggca aacttcagct aattctgctg gaatatcttg caattcgaac ttcatacctt
     1681 gagaagcctc atcccattgg atggctttca tggtgactaa gtcaacaaca ccttggaaag
     1741 tgtcttctgc gccaataggt acaaccattg gaacaggact tgcgcctaag cgaattttca
     1801 tttgctcaac agcgcggaag aagttagcac cagtacggtc cattttgtta acaaaggcga
     1861 tacgtggtac tttgtacttg ttagcttgac gccatacagt ttcggattgt ggttgtacac
     1921 cacctaccgc acagtacacc atacatgcac cgtccaatac acgcatagaa cgttctactt
     1981 caatggtgaa gtcaacgtga ccaggtgtat cgatgatgtt aatacgatgt tctgggaact
     2041 gatgagccat accactccag aaacaggtgg tcgcagcaga ggtaatggta ataccacgct
     2101 cttgctcttg ttccatccag tccatcgtgg ctgcgccgtc atggacttca ccgattttgt
     2161 ggtttacacc agtgtagtaa agaatacgtt cggtggttgt ggttttaccc gcatcaatgt
     2221 gcgcgctaat accgatatta cggtaacgct caagcggcgt agttctcgcc atgataatgt
     2281 cctcgttttc aaacctatcc cctgctcggg gataggcctt gttaaattag aaacgatagt
     2341 gagagaaggc tttgttagct tcagccatac gatggacatc ttcacgcttc ttaatagccg
     2401 cgcctttgcc ttcgtacgca tccaacaact cacctgctaa acgtagagcc attgtttttt
     2461 cgctacgttt tgcagcagca tctactaacc agcgcattgc taaagcagta cgacgggagg
     2521 cacgtacttc cataggcact tgataagtag caccgccaac acgacgagct tttacttcta
     2581 ccattgggcg cactttttcc aatgtgtctt caaagaagcg gactgggtca gctttacctt
     2641 tttcgagcac acggtctaaa gcaccgtaaa caataccttc tgctacagat tttttaccac
     2701 tgtccatgac gtggttgata aatttagcaa cagtttggct tttaaatttt ggatcgggca
     2761 gaatctctct agctgctacg acgcgtcttc ttggcatatc tatatgaacc tttaatgaac
     2821 ttcagggaag atctgggact tttttgccca gccttactac atcctcacaa tttttaagcc
     2881 gctaaggcca aaaaattatt tcttaggacg ttttgcaccg tatttagaac ggctttgttg
     2941 acggtttttc acgccagcac agtctaagga accgcgaacg gtgtggtaac gcacacctgg
     3001 taagtcttta acacgaccac cacggatcaa tacaacactg tgctcttgta agttgtggcc
     3061 ttcaccaccg atatacgaag aaacttcgaa accagaagtt aaacgaacac gacatacttt
     3121 acgtaacgca gagtttggct ttttaggtgt tgtggtataa acacgtgtac aaacgccgcg
     3181 acgttgtgga catgccttca acgcaggcac ttttgatttt tcgacaaggg tagtacgacc
     3241 tttgcgaatt aattgatttg ttgttgccat atcggcactc tctcccgtaa atactcaggc
     3301 ttatgcgttg cattgcaatt aacgcgtttt gtgagcgtta aggcatttta tgcctgccac
     3361 gcacacaaat aagcgataac agtataagcc ataaaaagaa aaaccctcaa aaagcctaca
     3421 aatagacttt ttgagggtgc attattttag ggatagcttg tctagtcgtc aagcctaaaa
     3481 aaatggccgt gagatcacgg ccatttttac tttttagcgt ttaaggctta ttcaccttgg
     3541 ctaaaggcat ctaataacgc ctgttctgcc gcttcttttt ctaagtcatg gttaagcact
     3601 tcattatctt ctgctttcac ttgaggcatc aaggcttcta aacgttgacg acgacgttct
     3661 gcgtgataag caaagcctgt acctgctggg atcaaacgac ccacaaccac gttttctttg
     3721 aggccacgta aatcgtcttc tttacctgtt accgcagctt cggttaagac acgggtagtc
     3781 tcttggaaag atgcagcaga gatgaaggag tctgttgcta aagaagcttt agtgataccc
     3841 attaacaagc gttcaaactt ggcaggaata tctccgtttt gttctaagac atcattttct
     3901 tcgacaacac gtgcgtactc aacttgctca cctttgatga atgaagagtc accaccatca
     3961 aggatgtcca ctttacgcaa catttgacga ataatcactt caatatgctt atcgttgatt
     4021 ttaacgcctt gtaaacggta aacgtcttgt acttcgttaa caatataagt tgccaacgcg
     4081 gtttcgcctt ttaagcgtaa gatatcatgt ggattttgtg gaccgtcaga aatgacttca
     4141 ccacgtgcca cgcgttcacc ttcgaagacg ttaatttgac gccattttgg aatcaactct
     4201 tcgtatggct ctgccccatc atctggcgta ataaccaaac gatgtttacc tttagtttct
     4261 ttaccaaagg aaactacacc actcttctcg gctaagatcg catgttcttt gggtttacgt
     4321 gcttcaaaca agtcggcaac acgtggcaaa ccaccggtga tgtcacgagt tttggacgat
     4381 tcttgaggaa tacgaccaat aacatcaccc acacctactt cggcaccatc ttgcaaactg
     4441 gtgattgcta aagctggtaa gaagtattga acaggctgat ctgagtcttt taagcgaata
     4501 tagttgcctg cggcatcaac caaagcaatc gctggacgca aatctttacc agaagctgga
     4561 cgatcttttg gatcaataat ttccatattg gttaagccag tctgttcgtc agtttgatga
     4621 cgtactgtaa tgccttcatc catatggata aatttcacac gaccagccac ttcagtcacg
     4681 attggatggg tatgtggatc ccagttagca actatttgac cgcccttcac ttcttgaccg
     4741 tctttaacgg taatcactgc accataaggc aatttgtagc gttcacgctc acgaccacgg
     4801 ctatcagcaa taccgatttc accagaacga gaaacagcga ctaagttacc attcgcatgt
     4861 tgaaccattt taatgttatg gaaacgaatc gtaccttcgt tacgcaccgt cacattacta
     4921 atcgcagaag ctcggttagc cgcaccaccg atgtggaacg tacgcatcgt taactgtgta
     4981 cctggttcac cgatcgattg ggctgccata acaccaatgg attcaccaat attcacttga
     5041 tgaccacgtg ccaaatcacg accataacaa ttagaacaca caccaaaacg ggttttacag
     5101 gtaattacgg agcgaacaat cacttcgtct acgcctgcta attctaattt gtctacccac
     5161 ttctcatcta acaacgtacc tgctggggca acgacttcat cagagtgcgg tgcaaccaca
     5221 tccacagcgg caacacgacc taacacacgc tcacgcaatg gctcaatgat gtcaccacct
     5281 tgaatcaatg gtgtcatgct taaaccttgg cttgtaccac aatctggctc agtaatgact
     5341 aagtcttgtg ccacatcaac caaacgacgg gttaagtaac cagagttcgc ggtttttaac
     5401 gcggtatcgg ccaaaccttt acgcgcaccg tgggttgaaa taaagtactg gagaacagtc
     5461 aaaccttcac ggaagttcgc tttaattggg gtttcgataa tcgagccgtc tggcttagcc
     5521 atcaaaccac gcatacctgc taactgacga atctgtgccg cgctaccacg agcaccagag
     5581 tcggccataa taaagatgga gttgaaggat ttttgcgatt cttcgacacc ttgtttattg
     5641 ataacttttt cagtcgacaa gttgtccatc atcgctttgg caactcgttc gttagtacgc
     5701 gaccaaatat cgacaacttt gttataacgc tcttggcccg ttaccaaacc tgcactatat
     5761 tgaccttcaa tttcacgtac ttcttcttcg gcatcgccaa tgatgacttg tttttcatct
     5821 ggaataacca tgtcttccat accaaccgac acaccagagt aagtcgcttg acggaaacct
     5881 aagtacatta attggtcagc aaaaataacc gtatctttta agcccaacac gcgataacag
     5941 gtgttgatta acttagagat atttttcttg gtcatgggtt ggttgataac attgtatggt
     6001 aagccttctg gcacaatgtt ccacaacata caacgaccag gtgtggtatc tgctaagaac
     6061 ttacgctctt gacggttacc atcgtcatca atcgtgactt cacgaacacg tactttaata
     6121 cgcgcatgaa tatcaactgc tttattggct aatgcacggg taacttcagc aaagtcagca
     6181 aagaccatgc cctcaccttt ggcattaatc ttgtcacggg agatatagta caaacccaat
     6241 accacgtctt gggatggtac aataattggc tcaccgttag ctggcgataa gatgttgttt
     6301 gtggacatca ttaacgcacg cgcttctaat tgtgcttcta aggttaacgg tacgtgtacc
     6361 gccatttggt caccgtcgaa gtcggcgtta aacgcggtac agactaatgg gtgtaactga
     6421 atcgctttac cttcgatcaa tacaggctca aacgcttgta aacctaaacg gtgcaaggtt
     6481 ggcgcacggt ttaacatgac cggatgttcg cgaataacat cagcaaggat atcccacact
     6541 tctgccgttt cacgctctac cattttcttg gcagctttaa tcgttgttgc caaacctttg
     6601 ctttctaatt tagcgaaaat aaacggcttg aataattcta aagccatttt cttcggtaaa
     6661 ccgcactgat gcaaacgtaa ggttggacct accacgataa cggaacgacc agagtagtca
     6721 acacgcttac caagcaagtt ttgacggaag cgaccttgtt tacccttaat catatcggct
     6781 aaggatttta atggacgctt gttgctaccg gtaattgcac gaccacggcg accgttatct
     6841 aataacgcat ctaccgcttc ttgcaacata cgtttttcgt tacgtacgat aatgtctggt
     6901 gcacttaact ctaataaacg cttcaaacgg ttattacggt tgataacacg acggtacaaa
     6961 tcgttaagat cagaggttgc aaaacgacca ccatccaacg gcactaatgg acgtaaatca
     7021 ggtggtaata ctggcaacac tgtcatcacc atccactctg gtttgttacc agaagacaag
     7081 aatgactcaa ctaattttaa acgcttagaa agctttttga gtttggtttc agagccagta
     7141 ttaggaattt cttcgcgtaa gcgttccatt tcagcttgca aatcaatgtc ttttaataaa
     7201 gcttgaattg cttctgcacc cattttggca tcaaactcat caccatattc ttctaaagcg
     7261 gtgtagtact cttcgtctga taataactga cctttttcta atggggtcat accaggctca
     7321 atcaccacaa agctttcaaa atacaataca cgctcaatat cacgtaaggt catatcgagc
     7381 aacaaaccaa tacggcttgg taaagatttt aagaaccaaa tatgcgccgt tggtgaggcc
     7441 aattcaatat gacccatacg ctcacgacgt actttagtga gggtaacttc aacaccacat
     7501 ttttcgcaga tcacaccgcg aaatttcatg cgcttatatt taccacataa acattcataa
     7561 tctttgattg gaccaaagat tttggcacag aacaaacctt cgcgttctgg cttaaaggta
     7621 cgatagttga tggtttctgg ctttttgact tcaccataag accaagagcg aatcatttca
     7681 ggcgaagcca aaccaatacg aatacggtca aattcgactt cttttccttg gctttgcaaa
     7741 atattgagca agtctttcaa ggctgtatct ccagcaaaag gcaagatagg ccaaacctat
     7801 cttgctcatg gtgcttattc gttgtctaac tcaatgttaa tacctaatga acgaatctct
     7861 ttaattaaca cgttaaagga ttcgggcata cctggctcca tacgatggtc gccatcaaca
     7921 atgtttttgt agatgcgggt acggccattg acatcgtcag acttgacagt gagcatttct
     7981 tgtaaggtat atgttgcgcc ataagcttca agcgcccaca cttccatctc accgaaacgc
     8041 tgaccaccga actgggcttt accacctaat ggttgttgag tcactaagga gtaagaacct
     8101 gtcgaacgcg catgcatctt atcgtcaacc aaatggttga gcttgagcat atacatataa
     8161 cccacagtca cttgacggtc aaaacgctca cctgtacggc catcatataa ccattgttga
     8221 ccattttcag attgatcggc taaacgcaat aaggctttaa tttcggtttc atgtgcgccg
     8281 tcaaaagcag gcgttgcaat cggcacacca ccacgtaagt ttttggccaa agccatgact
     8341 tcgttgtcac ctaactcttc taagctttct tgctcaccac caacttggtt atagatcttg
     8401 tctaagaact cacgtaactc agcggcttga cgttgttgat ccaacatctt accaatcttc
     8461 gtacctaagc ctttagccgc ccaacctaag tgggtttcta aaatctgacc aacgttcata
     8521 cgcgatggaa cgcccaatgg gtttaacacc acatcaactg gctcaccatt ttcgtcatac
     8581 ggcatatctt caacaggcat aatcactgag accacacctt tgttaccgtg acgaccggcc
     8641 atcttatcac ctggttggat acgacgctta accgctaagt acactttaac aactttcaat
     8701 acgccagttg ttaaatcatc acctgtggtg attttgcgtt ttttatcttc aaacttgtct
     8761 tcgatttctt cttgacgttc ttgaagatac gcttgtacac gctctaattg atcagcaacg
     8821 ctatcatcaa caggacgaat atcaaaccat tgatctaact ttaagccact gaggttgtct
     8881 tcagacaaca catcgccttt cttaaagcct gcaccaccat tcacttcttg accttttaac
     8941 aaggacaata aacggctacg cgcagcttct tcaaaaatac gatattcttc ttttaagtct
     9001 ttacggtaag cattgagttg ttgtttctca atcgctaagg cacggctgtc tttatcaaca
     9061 ccgtcacggg taaagacttg tacgtcaata accgttcctt tagtaccaga aggcacacgt
     9121 aaagaactgt cttttacatc agacgctttt tcaccgaaga tagcacgtaa taacttctct
     9181 tctgggctga gttgagtttc accttttggt gtcactttac caaccagaat atcgccagca
     9241 ttcacttcag caccaatata aacgatacct gattcgtcta atttggataa tgcagcttca
     9301 ccaacgtttg gaatatcggc agtgatttct tcagggccta acttcgtatc acgggcaata
     9361 caagacaact cttgaatatg aatagacgtg aaacggtctt cttgcaccac acgctcagat
     9421 aacaaaatcg agtcttcgaa gttgtaacca ttccaaggca tgaacgcaac acgcatattt
     9481 tgacccaagg ccaattcacc taagtcaaca gatggaccgt cagctaaggt gtcgccacga
     9541 gcaaccacat caccacgatt cacgatagtg cgctggttaa tacaagtgtt ttggttagaa
     9601 cgggtgtact tggttaagtt gtaaatatca acgcccgctt cacccgcaac catttctgac
     9661 tcatcaacac ggataacgat acgcgctgcg tcaacataat caatcacacc accgcgtttt
     9721 gctgtcacac acacaccaga gtcacgcgca acataacgtt ccatacctgt accgactaaa
     9781 ggtttgtcag cacgtaaggt tggcacagct tgacgttgca tgttagaacc catcaacgca
     9841 cggttcgcgt cgtcgtgttc taagaacgga atcaaggaag ccgcaacaga aactacctga
     9901 cgtggtgaaa cgtccatatg ggtaactttg tcgggtgcca taacggtgaa ttcgttttta
     9961 tgacgaacag caaccaagtc atctaacaaa cgaccttcgc tatcaacggc ggagtccgct
    10021 tgcgcaacca cgcattggct ttcttcaatc gcagacaagt aaacgatttc gtcttgtact
    10081 tgaccatcag ccaccttgcg ataaggagac tctaagaagc catagctatt ggattgagca
    10141 aataccgcta aggagttgat caaaccaatg tttggacctt caggcgtttc gattggacaa
    10201 acacgaccat agtgagttgg atgaacgtca cgcacttcaa agcctgcacg ctcacgggtt
    10261 aaaccaccag ggcctaaggc agaaacacga cgcttgtgag taatctcaga caatggattg
    10321 ttttgatcca taaattgaga taattggcta gaaccgaaga attctttaat cgccgcagaa
    10381 acaggtttag cgttaattaa atcttgtggc attaagtttt cgctttcagc caagcttaaa
    10441 cgctctttaa ctgcacgctc aacacgtacc aaacccacac ggaattggtt ttcggtcatt
    10501 tcaccgacac aacgcacacg acggttacct aagtggtcga tgtcgtcgat ttcgccttta
    10561 ccattacgga tatcaattaa agtttttaat acatcaacga tatcttgttt agataatacg
    10621 ccatcttttt ccatgctatc gccattacca cggtcattac cgcgatttaa acgacgattg
    10681 aacttcatac gacctacacc agataagtca taacgttctt gggtaaagaa taagttttta
    10741 aataaggctt ctgctgcgtc ttttgtgggt ggctcaccag gacgcatcat gcgataaatt
    10801 tcaactaaag catctaattc ggtacgtgtt gacgcatcag cacgtaaagt gtcagcaata
    10861 aacgcaccac gatcaatatc gttggtgtat aaaatattga actgaccaat acctgctttc
    10921 gctaaacgag ttaatacttc agcggtaata atggtattac cttcgcaaac caactcgcct
    10981 gttgcaggat gagctaaagt cttagcaatc actttaccat gcaaataatc agcaggaata
    11041 tctaactcag ttaagcctgc tttttgaatt tcacggatgt gttttggtgt aatacgacga
    11101 cctacttcaa caatcacttt ctctttagaa gcaatctcaa aagccgctgt ttcaccacgt
    11161 aaacgctcag caatcaaacc taatttgtaa gtaccatctt catgtttaat tgaagtggtt
    11221 tcaaagaaca tttctaagat ttgatcagag ttataaccta aaccacgtaa taaaatggtg
    11281 gcatataact tacgacggcg gtcaatacgc acatacacga ggtcttttgc atcgaattcg
    11341 aagtctaacc aagaaccacg gtaaggaatc acacgcgcat taaacaacaa cttaccagat
    11401 gagtgtgttt ttcctttgtc atgatcaaag aacacacctg gactacgatg taattgcgaa
    11461 acgataacac gttctgtacc gttaatcaca aacgtaccgt tatccgtcat caatggcatt
    11521 tcgcccatat agacttcttg ctcacggata tctttaatgg ttttcacagc cgcttcacgg
    11581 tcataaatca ccaaacggat tttgacacgt aaaggtgcag cataggttaa accacgtaaa
    11641 atacattcac gaacatcaaa aactggatgt cctaaatgat attccacaaa ctctaatgca
    11701 gcattacctg aataactttg aattgggaat acagatttaa aagcggcctg taaaccaaca
    11761 tcttcacgtt gttttgcttt tttaccatct tgtaaaaacg cacgataaga atccacttgg
    11821 atgcctaata aatgtggcac atccattaag gtgggcaatt tgccgaagtt ttttc
//
LOCUS       DAOQMB010000030        11627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2140_length_11627_cov_15.632129, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000030 DAOQMB010000000
VERSION     DAOQMB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2140_length_11627_cov_15.632129"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLY05_02285"
     CDS             <1..789
                     /locus_tag="PLY05_02285"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNC02160.1"
                     /translation="ALILVPIVIWCIFFANGHIPFMAFAGAIVLVGAWEWTALMRWTS
                     MPLRAMYVAVVALGLVAMNIPQLVFLKQPFYMVSVGFWLVALRWVMAFPESAKIWARN
                     ILLVPTGLILLIPAWAGLTDLHQASPWWLLYVFCLVWGADTGAYFAGRKFGKHKLAPS
                     VSPGKTIEGFVGGLITTSMLIVAVAIYRDLSAIRFVAFVGLSLLTVLASVLGDLLESM
                     VKRQAGVKDSGNIFPGHGGALDRIDSLTAAAPLFALGWWLAGGF"
     gene            765..1955
                     /gene="ispC"
                     /locus_tag="PLY05_02290"
     CDS             765..1955
                     /gene="ispC"
                     /locus_tag="PLY05_02290"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNC02161.1"
                     /translation="MVVGRRFLMQRITILGATGSIGQNTLAVIALHPDRYQVFALTAH
                     TRLDELAKLCQQYQPRYAVIQHEQDIPKLQALLAQSQTEVLAGQQALAWVAAHEQCDT
                     VMAAIVGAAGLLPTLAAVKAGKRVLLANKEALVMAGQVFMQAVTDHQATLLPIDSEHN
                     AIFQCLPNTYQQGLSKVGVERILLTASGGPFREFSAEQLQNVTPEQACKHPNWVMGRK
                     ISVDSATLMNKGLEFIEACWLFDAKPSQVQVVIHPQSIIHSLVQYIDGSTLAQLGNPD
                     MRTPIANALAYPERIQAGVSALDLCQHQLQFYPPDESRFPCLALARQAMQTGGTAPAM
                     LNAANEVAVAAFLAGKLPFIAISQLIDTVLTQLPMSSAHNLDTILQADQQARALAQQK
                     LSSF"
     gene            1970..3334
                     /gene="rseP"
                     /locus_tag="PLY05_02295"
     CDS             1970..3334
                     /gene="rseP"
                     /locus_tag="PLY05_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004653311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNC02162.1"
                     /translation="MSILQIILAAIIVLGPLIAIHEFGHFWTARRLGVKVLTFSIGFG
                     PALAKWRGKDGTDYQIAAIPLGGYVRMADEREGEVPAEDIPKAFNRQPVWARMAIVAA
                     GPLINLAFAVFLFWVLFLQGTETLKTIVGQVKPNTPAAIAGLKVGDEIVAVDGKQTHD
                     WEAITYALVNRVGENGTVTLSVNSANATTTKDVQLPIKNYLSQSGQDPLLSLGFTPYQ
                     PPLKPIIGEVVASGAAAQQGLKAGDKILNAQGQVINTWQDFVEIVRTSPEKSFPVIVE
                     RAGQPVNLTLTPRMERDEVGNKEGKLGIALQQQPIDIPPQYIQQRQYNPWTALVKACQ
                     KTYDLIVMTLSSLGKMIMGLIGLDNLSGPITIAKVAGHTANIGWQAMIGFMALLSVSL
                     GVLNLLPIPVLDGGHLVYYAAEGIMGRPLSEQVQQMGLKIGMAMMGCLMFLAIFNDLS
                     RLFG"
     gene            3346..5640
                     /gene="bamA"
                     /locus_tag="PLY05_02300"
     CDS             3346..5640
                     /gene="bamA"
                     /locus_tag="PLY05_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNC02163.1"
                     /translation="MISQRLLLTTAALCLSAQAVAETGSFTVKDIQIQGLMRVSPASV
                     YAQLPINTGDVVDDAKIALAIRQLFATGSFEDISLARDGDVLIVNVIERPSIASIKLE
                     GNKAIDTDTLMKALKDAGLVEGEVLKRASLDHIKGELERVYFGQGRYDATISVNNIAK
                     PRNRVAVDVKINEGESARITTINILGNKAFSEKELLKLMQSKATHFTSFFKGDDKYAR
                     ERLMGDLEALRSFYLDSGYINFAINSHQVTLSQDKKSVFIDISLNEGDKYQFGEPKIL
                     GEVPINEQELQKLLLVKSGRDYSQQLVTASSKLMKQRLGSDGYLFSEINPIPEIDEQN
                     KKVNLSFFVNPNKKTYVRRVNFQGNNKTDDHVLRREMRQFEGTLASTDKIDLSKLRLQ
                     RLGFFKDVTIETPKVPNTTDQVDLNVKVQEQPSGTLTASVGYSGGSGMIFSLGVSQNN
                     FLGTGNKVGINLNRSETSDNYNISFSDPYFTVDGISRGYNIYYRNTKLDERNISRYAT
                     DSQGASLSFGYPIDETKNINFSLGIDQTDITLGTLPSQLVQNFVTEHGNSITSYMGVV
                     SWNYNSLNRGVFATKGASQRLALEFAVPPSDVNYTKLSYAAQIYFPINEDFTVRLRTD
                     LGYGDSLPFYKNYFAGGYGSVRGYRDNTLGPRSPVIANDPDPEVVGGNVLVENSVELI
                     VPTPFAKNYSQLRTVFFVDSGMVYYRNNPPYNFDLSNLRYSAGISIAWLTAIGPLSFS
                     LSRPFNYDEADDRQNFQFTIGQPF"
     gene            5668..6168
                     /locus_tag="PLY05_02305"
     CDS             5668..6168
                     /locus_tag="PLY05_02305"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNC02164.1"
                     /translation="MRLASYLIAGLLTTIAMPALAGNVAVADSQAAIMATETAKKTFE
                     KLNNDMKPQRDRLEQLRKDIAAIEEKFQKNASVMSDKDKRDLQKQAESKLNEFKNLAD
                     AVQKRANEVQQEMLKTLIPKTEVVVEEIRKAGNYDIIIEKKNVIFADPAVDITKKITE
                     KLNAAK"
     gene            6263..7294
                     /gene="lpxD"
                     /locus_tag="PLY05_02310"
     CDS             6263..7294
                     /gene="lpxD"
                     /locus_tag="PLY05_02310"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNC02165.1"
                     /translation="MWTLASIAKQIGGTVKGQLEYPISGLGTLKNAQSHQLSFLANPL
                     YRSALASTQAGAVLLRAEDAENYQGQAIIVASPYAAYAQLTHFFDQTPKGIVGIHSQA
                     IVSDSAKVSETAVIAACAVISDEAVIGDGTIIESGAFIGARVKVGKNCRIRANAVIHH
                     DCVLGDRVTIHSGAVLGDDGFGFAPHQGRWFKIAQIGRVVVHNDADIGSNTTIDRGAL
                     DDTIIHEGAIIDNQVQIAHNVEIGAHTAIAACCGISGSSKIGAHCVLAGGVGLVGHIE
                     ICDNVHITGMTMITKSITQAGSYSSGTAFDSTDNWKKMAVRLRKLDDMAKRLRELEGI
                     VQRLTQDQG"
     gene            7404..7862
                     /gene="fabZ"
                     /locus_tag="PLY05_02315"
     CDS             7404..7862
                     /gene="fabZ"
                     /locus_tag="PLY05_02315"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011280792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HNC02166.1"
                     /translation="MTEQIKLPLHIADIKALLPHRYPFLLVDRVTEIELGRSVKGYKN
                     VTINEEFFGGHFPDYPVMPGMLILEAMAQIAGVLGFLTSGKRPADGHIYLLVGTDNTR
                     FKRQVVPGDRLTLEAEFVTSKRHIHKFSCRAMVGDELAASADILVAEQVI"
     gene            7863..8639
                     /gene="lpxA"
                     /locus_tag="PLY05_02320"
     CDS             7863..8639
                     /gene="lpxA"
                     /locus_tag="PLY05_02320"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HNC02167.1"
                     /translation="MNIHPTAIIDPSAHIADDVTIGPYSIIGANVTIGSGCEIGPHVV
                     IKGHTTIGKNNRIFQFATVGEDCQDKKYKGEQTFLEIGDNNMIRENCTIHRGTVQDNS
                     ITRIGSGNLFMVNTHIAHDCIVGDDCIFANNSGIAGHVKVGNSVIIGGNTGVHQFCQI
                     GSYAMTGGASLVLKDIPAYVMVFGNPAEATGMNFEGMRRRGWTSETIGLLKRAYKIVY
                     RQGLTLEAAISQLEQDILPNCPEAAIFIDSLKASTRGITR"
     gene            8731..9987
                     /locus_tag="PLY05_02325"
     CDS             8731..9987
                     /locus_tag="PLY05_02325"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HNC02168.1"
                     /translation="MFDYHKDQHAAASTQDYVFQQLIPYIGNKRRALPMIFQAIQHTG
                     INPRQASFLDLFAGSGVVSRMAKKCGFKVLTNDWEPYATALNQSYIACNYEPAFLELG
                     GYENVIAYLNALNPIEDWITRHLCPTHDQYFDTRVDRLFYTRANGQRLDAMREQIEWW
                     DEQQLITPYEKACLLAPLLFQASYVSNTSGVFKAFHQGWGGKTGTALSRILSVVELRP
                     IHFFDNQRANQVYQMDGLELAQQLTQMGIEVDIAYLDPPYNQHCYGSNYHVLNSVTLW
                     DKPILSEKIQGRNKAAIRQDWRERRSPYNYRGEAEQAFEGLLANLQAHYILASYSTEG
                     LISLENFVALALMKGHTELLCYAHKRYRVSTPRFSPKPMTVEFILIINSHKKHRGLSH
                     SALCAKIRQTEQNALLHYEYQHEAQH"
     gene            9959..11140
                     /gene="lpxB"
                     /locus_tag="PLY05_02330"
     CDS             9959..11140
                     /gene="lpxB"
                     /locus_tag="PLY05_02330"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017389545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNC02169.1"
                     /translation="MNINTKPNTDNRPITIGIVAGEISGDTLGAGLIKALKQRYPHAR
                     FVGICGAQMIAEGGETWFPLERLSVMGLVEVLGRIKELFAIRDELVRRFVAEKIDVFI
                     GIDAPDFNLRVAPQLKQAGIKTVHYVSPSVWAWRQGRIHGIKAAIDLMLCLLPFEKQF
                     YDQHQLDAVFVGHPLADSLPLKNDTLAVRQQLGLEANKQYVALLPGSRGGEVSRLAEP
                     LFAAALLLKKQHPQLEFIIPAINALRKKQISELLEQSGLQATIFDDSYGAGVGRLVMA
                     AADVVVLASGTATLEAMLLKKPMVVVYKLHWLTYLIVRLMAKAKYISLPNLLANQALV
                     PELVQHAVTPESISQAVEQWLKPQQQQQIVARFTELHQQLQANGSETAAQAISQLIEK
                     Q"
     gene            11137..>11627
                     /gene="rnhB"
                     /locus_tag="PLY05_02335"
     CDS             11137..>11627
                     /gene="rnhB"
                     /locus_tag="PLY05_02335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNC02170.1"
                     /translation="MMELAEILQKYQHIAGVDEVGRGPLVGAVVTAAVILDPNNPIEG
                     LNDSKKLSEKKRLLLAEEIKAKALAWSLGRAERHEIDELNILRATMLAMQRAVNGLKI
                     HPDFVLIDGNKCPILTCPAQAVVKGDSLVPAISAASILAKVTRDAEMDVLHQQYPQYG
                     FAD"
BASE COUNT         3383 a         2066 c         2626 g         3552 t
ORIGIN      
        1 gctttaattt tagtcccgat tgtgatttgg tgtatttttt tcgccaatgg tcacattcct
       61 tttatggcgt ttgcaggtgc cattgtgctt gttggcgcat gggagtggac agctttaatg
      121 cgttggacat ctatgccatt aagagcgatg tatgttgctg ttgttgcctt aggtttggtg
      181 gcgatgaaca tcccgcaact ggtattttta aagcagcctt tttatatggt gtcagtaggt
      241 ttttggttgg ttgctctacg ttgggtgatg gcctttcctg aatcagccaa aatttgggca
      301 agaaatattc tcttagtgcc aacgggtttg attttattaa ttccagcatg ggcaggactt
      361 accgatttac accaagcgtc tccttggtgg ttgttgtatg ttttttgttt ggtatggggt
      421 gcagatacag gcgcttattt tgcaggtcgc aaatttggca aacacaaact tgcacccagt
      481 gtcagtccag gtaaaaccat tgagggtttt gtcggcggtt taatcaccac tagtatgtta
      541 attgtggcag ttgcgattta tcgtgattta tccgcaatac gctttgtggc ttttgtgggc
      601 ttatcattat taaccgtgtt ggcttctgtg ttgggtgact tattagaatc tatggttaag
      661 cgtcaagcag gagtcaaaga ttcaggcaat atttttcctg gtcatggcgg tgcgttagac
      721 cgtattgata gcttaacagc cgcagcacct ttatttgctt taggatggtg gttggcagga
      781 ggtttctaat gcaacgaatt accattttag gtgcaactgg ttcaattggt caaaatactt
      841 tagcggttat tgctttgcat cctgatcgtt atcaagtctt tgccttgact gcacatactc
      901 gcttggacga attagcgaaa ttatgtcagc aatatcagcc acgttatgct gtgattcaac
      961 atgagcaaga tatccctaaa ttacaagcgt tattagctca aagccaaaca gaagttttag
     1021 caggtcaaca agctttagca tgggttgctg cccatgagca atgtgatacg gtcatggcag
     1081 ctattgttgg tgcagcaggc ctattgccta ccttagccgc agtcaaagca ggtaaacgcg
     1141 tattattggc caacaaagaa gccttagtga tggctggtca agtgtttatg caggcagtta
     1201 ccgaccatca agccacgtta ttaccaattg actctgaaca taatgccatt tttcaatgtt
     1261 tgcccaatac ctatcaacaa ggcttaagca aagtcggtgt cgagcggatt ttgttaacgg
     1321 catcaggtgg gccatttcgt gaatttagtg ctgaacaatt acaaaatgtc acgccagagc
     1381 aagcatgtaa acaccctaat tgggtaatgg ggcgtaagat ttctgtggac tcagccacct
     1441 taatgaataa aggcttagag tttattgagg cttgctggtt gtttgatgct aaacctagcc
     1501 aagtgcaagt ggtgattcat ccacaaagta ttattcactc cttggtacaa tatattgatg
     1561 gttcgacctt agcgcagttg gggaatcccg atatgcgtac gccgattgcg aatgccttag
     1621 cttatcctga gcgtattcaa gcaggggttt ccgcattaga cttgtgtcag catcaattac
     1681 aattttatcc acctgatgaa agccgttttc cgtgtttagc cttagcgcgt caagccatgc
     1741 aaacaggtgg cacagcacca gccatgttaa atgcggctaa tgaagtggca gttgccgcat
     1801 ttttagcagg aaagttacct tttattgcga ttagtcaact gattgatacg gtacttacac
     1861 aattaccaat gagtagtgcg cataatctcg acacaatact ccaagctgac caacaagcac
     1921 gagcattggc acagcaaaaa ctttcttcgt tttaaaatta gtattcagta tgagtatttt
     1981 gcaaatcatt cttgctgcta ttattgtgtt ggggccatta attgccatcc atgagtttgg
     2041 ccatttttgg acagcacgac gtttgggcgt gaaagtatta actttttcta ttggttttgg
     2101 ccctgcctta gcaaaatggc gcggtaaaga tgggacagac tatcaaatcg ccgctatccc
     2161 tttagggggc tatgtacgca tggccgatga acgtgaaggg gaagtgcctg ctgaggatat
     2221 tcctaaagcc tttaatcgcc aaccagtatg ggcaagaatg gcgattgttg ccgcaggtcc
     2281 gctcattaat ttagcgtttg ccgtgttttt attttgggta ttgtttttac aaggcacaga
     2341 aacgctaaaa acaatcgttg gccaagtaaa acctaatact cctgccgcga ttgctggtct
     2401 aaaagtaggt gatgaaattg ttgcggtaga tggcaaacaa actcatgatt gggaagcgat
     2461 tacttatgct ttagtcaatc gtgttggtga aaacggtacg gttactttaa gtgtcaattc
     2521 tgctaatgcg acgactacaa aagatgtgca gttacctatt aaaaactatt tgagtcagtc
     2581 aggccaagac cccttattaa gtttaggttt tacaccctat caaccaccat taaaaccgat
     2641 tattggtgaa gtcgtggcga gtggcgcggc tgcccaacaa ggtttaaaag ctggcgataa
     2701 aattttaaat gcgcaaggac aagtgattaa tacatggcaa gattttgttg aaattgttcg
     2761 tactagccct gaaaaaagct ttccagtgat cgttgaacgg gcaggacagc cagttaattt
     2821 aacgctgaca ccgcgcatgg aacgtgatga agtaggtaac aaagaaggca agttaggtat
     2881 tgccttacaa caacaaccga ttgatattcc tcctcaatat attcaacaac gtcaatacaa
     2941 tccgtggaca gccttagtca aagcttgcca aaaaacctat gatttaattg tcatgacctt
     3001 aagctcttta ggtaaaatga ttatgggttt aataggacta gataatttga gtgggccaat
     3061 taccattgct aaagttgcag gacataccgc caatattggt tggcaagcca tgattggttt
     3121 tatggcgttg ttgagtgtca gtttaggggt attaaattta ttaccaatac ctgtgttaga
     3181 tggtggacat ttagtatatt acgcagccga agggattatg ggacgacctt tgtccgaaca
     3241 agtgcagcaa atggggctaa aaataggcat ggcaatgatg ggctgcctca tgttcttagc
     3301 aatttttaat gatttaagtc gattatttgg ttagtggatt cctttatgat ttctcaacgg
     3361 cttttgttaa caacagccgc actgtgtttg tctgcgcagg cggtggctga gactggctca
     3421 tttacggtaa aggacattca aattcaagga ttgatgcgtg tttcgccagc gagtgtgtat
     3481 gcacaacttc cgattaatac aggcgatgtg gtagatgatg ccaaaattgc gctagccatt
     3541 cgtcagctct ttgctacagg cagctttgaa gatattagtc tagctcgaga cggtgatgta
     3601 ttaattgtca acgtcattga acgcccttcc attgctagta ttaaattaga aggtaataaa
     3661 gcgattgata ccgatacctt aatgaaggca cttaaagatg ccggtttggt tgaaggtgaa
     3721 gtattaaaac gtgcatcctt agatcatatt aagggagagt tagagcgcgt gtattttggc
     3781 caaggtcgtt atgatgcaac gattagtgtc aataatattg ctaagccacg caatcgcgtt
     3841 gccgttgatg tcaaaattaa tgagggtgaa tctgcacgaa tcacgacaat taatattttg
     3901 ggtaataaag cctttagtga aaaagagcta ctaaaattaa tgcagtctaa agccacacat
     3961 tttacttcat tttttaaagg tgatgataaa tatgcccgtg agcgactaat gggcgattta
     4021 gaagcattac gttcatttta tttagatagt ggctatatta attttgcgat taactcgcat
     4081 caagtcactt taagccaaga taaaaaaagt gtttttattg atattagttt aaatgaaggt
     4141 gacaaatatc aatttggaga gcctaaaatt ttgggtgaag ttcccattaa cgagcaagag
     4201 ttacaaaaat tattactcgt taaatcgggt cgagactatt cacaacagct agtcacagca
     4261 agtagtaagc tgatgaaaca gcgtttaggt agtgatggtt atttatttag tgaaattaat
     4321 cctattcctg aaattgacga acaaaataaa aaagttaatt taagcttttt tgtcaatccc
     4381 aataaaaaga cttatgtacg tcgagttaat tttcaaggca ataataaaac agacgatcat
     4441 gtgttgcgtc gagagatgcg tcaatttgaa gggacgttgg cctcaaccga taaaattgat
     4501 ttatcaaaat tacgtttaca acgtttaggt ttttttaaag acgtgacgat tgaaacacct
     4561 aaagtaccta ataccaccga tcaagttgat cttaatgtta aagtacaaga acaaccatca
     4621 ggaacattaa ccgctagtgt cggttattca ggtggttctg gcatgatttt tagtttaggg
     4681 gtaagtcaaa ataacttttt aggcacaggt aataaagttg gtattaattt aaaccgttca
     4741 gaaacgagtg ataattataa tatttctttt tctgaccctt attttaccgt tgacggcatt
     4801 agtcgtggtt ataatatata ttaccgtaat actaagttgg atgagcgtaa catcagccga
     4861 tatgcaacag actcacaagg agcgagtttg agttttggtt atccaatcga tgaaaccaaa
     4921 aatattaatt tttctttagg gatagatcaa acagatatta ccttgggcac tttgccatct
     4981 cagttagtgc aaaattttgt tacggagcat ggcaatagta tcacctctta tatgggcgtt
     5041 gtatcgtgga actacaatag tttaaaccga ggtgtgtttg cgactaaagg tgcttctcag
     5101 cgtttggcat tagagtttgc ggtaccacca agtgatgtta actacacaaa attgtcgtat
     5161 gcagcacaga tttattttcc gattaatgaa gattttaccg tacgcttacg taccgattta
     5221 ggttatggtg atagcttacc attttataaa aactattttg caggtgggta tggttcggta
     5281 cgtggttatc gtgataatac cttaggccct cgtagtccgg tgattgccaa tgatcctgat
     5341 ccagaagtgg ttggtggtaa tgttttagtc gaaaattcgg ttgaattgat tgtacccaca
     5401 ccatttgcta aaaattatag ccaattacgt acggtatttt ttgttgattc aggtatggtg
     5461 tattatcgta ataatccacc ttataatttt gatttatcaa atttgcgcta tagtgcaggt
     5521 atttcgattg catggctaac agccattggg cctttatctt ttagtttgtc tcgtccattt
     5581 aattatgatg aagccgatga tcgacaaaat ttccaattta ccattggtca accattttaa
     5641 ctttttacaa caataggaga agagggaatg cgtttagctt cttatttaat cgctggttta
     5701 ttaacaacca ttgccatgcc tgcattggct ggtaatgttg ctgtagcaga tagccaagca
     5761 gcgattatgg caactgaaac ggctaaaaaa acctttgaaa aacttaacaa cgacatgaag
     5821 cctcagcgtg atcgtttaga gcaattgcgt aaagatattg cggctattga agaaaaattt
     5881 caaaaaaatg ccagtgttat gagcgataag gataaacgtg acttgcaaaa acaagcagaa
     5941 agcaaattaa atgagtttaa aaatttagca gatgctgtgc aaaaaagagc taacgaagtt
     6001 cagcaagaaa tgttaaaaac cttaattcca aaaacagaag tcgttgttga agaaattcgt
     6061 aaagcaggca attatgatat tattattgaa aagaaaaatg tcatttttgc tgatccagcg
     6121 gttgatatca ccaaaaaaat cactgaaaaa ttaaatgctg cgaaataaat agtagtaagc
     6181 acgattaaac agcttttaaa ttaaaaaggc tagcttgtat agatcaatgc tagcattttt
     6241 attttgggga ctagggggat ttatgtggac attagcttca attgctaaac aaataggcgg
     6301 aactgttaaa ggtcagttag agtacccaat atctggttta ggtacactaa aaaatgccca
     6361 atcacatcaa ctaagttttt tagctaatcc tttatatcgt tctgctttag cgtctaccca
     6421 agctggagct gttctattac gtgcagaaga tgcagaaaac tatcaaggtc aagcgattat
     6481 tgtggcaagc ccttatgctg cttatgccca actaacccat ttttttgatc aaacacccaa
     6541 aggaattgtt ggtattcatt ctcaagcgat tgttagtgat tcagcaaagg tttcagaaac
     6601 agcggtaatt gctgcatgtg cagtgattag tgatgaggca gtgattggtg acggtacaat
     6661 cattgaatct ggtgcattta ttggtgcacg ggtaaaagtt ggtaaaaatt gtcgtattcg
     6721 tgccaatgcc gtgattcatc atgattgtgt gttgggcgat cgagtcacaa ttcactcagg
     6781 tgcggtgctg ggtgatgatg gctttggttt tgcacctcat caaggtcgat ggtttaaaat
     6841 tgctcaaatt ggtcgagtgg ttgtgcataa tgatgccgat attggttcca ataccaccat
     6901 tgatcgtggc gcgttggatg acaccattat tcatgaaggt gcgattattg ataaccaagt
     6961 acaaattgct cacaatgttg aaatcggtgc acatactgca attgctgctt gttgtggcat
     7021 ctcaggtagt agtaaaatcg gcgcccattg tgtgttggca ggtggtgttg gtttagttgg
     7081 tcatattgag atatgcgata atgtccatat tacaggcatg accatgatta ccaagtcgat
     7141 tacacaagca ggtagttact catcaggtac tgcctttgat agcaccgata attggaaaaa
     7201 aatggctgtt cgtttaagaa agttagacga tatggcaaaa cggttgcgcg aattagaagg
     7261 gattgtgcaa cgactaactc aagaccaagg ttaaggccga ggttttattg ctgggcttgc
     7321 gcactatatt cggtgaagtt cgtaagtctc acagtgtatt tactatcgaa aatggctagt
     7381 aaatcctaaa cttgagattg attatgactg agcaaataaa gttgcctttg catatagccg
     7441 acattaaagc cttattgcct catcgttatc catttttatt agttgatcgt gtcacagaaa
     7501 ttgaattagg acgttcagtc aaaggttata aaaatgtcac cattaatgaa gagttttttg
     7561 gtggccattt tcctgattat cctgtgatgc cgggtatgtt aatcttagag gcgatggcac
     7621 aaattgcagg cgtattaggg tttttaacca gtggtaaacg acctgctgat ggtcatattt
     7681 atttgttagt aggcactgat aatacccgtt ttaaaagaca agtcgtaccc ggtgatcgct
     7741 taaccttaga ggctgagttt gtaaccagca aacgacatat tcataaattt tcttgtcgcg
     7801 caatggttgg cgacgaattg gccgccagtg ctgatatttt agtggccgag caggtaatct
     7861 aaatgaacat tcatccaaca gcaattattg atccttctgc acacattgcc gatgacgtaa
     7921 cgattggccc ctattcgatt attggcgcaa atgtcacgat tggtagtggt tgtgaaattg
     7981 gcccacatgt tgtgattaag gggcatacca cgattggtaa aaataatcgt atttttcaat
     8041 ttgccacggt aggggaagat tgccaagata aaaaatataa gggtgaacaa acctttttag
     8101 aaattggcga caataatatg attcgcgaaa actgtacgat tcatcgtggt acagtgcaag
     8161 ataacagtat tactcgtatt ggtagcggta atttattcat ggtcaatacc catattgccc
     8221 atgattgtat tgtcggtgat gattgtattt ttgctaacaa ctcaggtatt gctggccatg
     8281 taaaggtggg caatagtgtg attattggtg gaaataccgg tgtacatcaa ttctgtcaaa
     8341 ttggctctta tgcgatgaca ggcggtgcaa gcttagtctt aaaagacatt cctgcttatg
     8401 taatggtgtt tggtaatcct gctgaagcaa caggcatgaa ttttgaaggt atgcgtcgtc
     8461 gtggttggac aagtgaaacc attggtttgt taaaacgtgc ctataaaata gtgtatcgtc
     8521 agggtttaac cttagaagcg gcaatcagtc agttagagca agatattttg ccaaattgtc
     8581 ctgaagcagc aatctttatt gactccttaa aagcctccac tcgcggtatt acccgttaat
     8641 atttagactc caccctaacc ttcccctaaa ttaggggagg gaatatatca gcatcttaga
     8701 taagcctttt cttgcgcctt gagttagcta atgtttgact atcataaaga ccaacatgcg
     8761 gcagccagta cgcaagatta tgtgtttcag caactcattc cgtatattgg taataagcgc
     8821 cgtgccttac caatgatctt tcaagcaatt caacatacgg gcattaatcc acgccaagcc
     8881 agttttttag atttgtttgc aggcagtggt gtggtctctc ggatggcaaa aaaatgtggt
     8941 ttcaaggttt taaccaatga ttgggaacct tatgcgacag cactcaatca aagttatatt
     9001 gcctgtaatt atgagcctgc atttttagag ttgggtggtt atgagaatgt cattgcctat
     9061 cttaatgcct taaaccccat tgaagactgg attacccgtc atttgtgccc aacccatgat
     9121 cagtattttg atacacgtgt tgatcgtttg ttttataccc gtgctaatgg tcagcgtttg
     9181 gatgcaatgc gtgaacaaat agaatggtgg gatgagcaac agttaataac cccttatgaa
     9241 aaagcgtgtt tattagcacc tttattattt caggcgagtt atgtcagcaa taccagtggc
     9301 gtttttaaag cctttcatca aggttggggc gggaaaacag gcactgcctt aagtcgaatt
     9361 ttgtcagttg tcgaattgcg acctattcat ttttttgata atcaacgtgc gaatcaagtg
     9421 tatcaaatgg atggtttgga attagcacaa caactgacac aaatgggtat tgaagttgat
     9481 attgcctatt tagacccgcc ctataatcaa cattgttatg gcagtaatta tcacgtttta
     9541 aatagcgtca ctttgtggga taaaccaatt ttaagtgaaa aaattcaagg acgaaataag
     9601 gctgcaattc gccaagattg gcgcgagcga cgcagtccat acaactatcg cggtgaagca
     9661 gagcaagctt ttgaaggctt attggcaaat ttacaagcgc attatatctt ggcgagctat
     9721 agcacagaag gcttgatttc tttagaaaat tttgtcgctt tagcactcat gaaagggcat
     9781 actgagttat tatgttatgc tcataaacgt tatcgagtca gcacaccacg tttttcacct
     9841 aaaccgatga ccgtagagtt tattttaatt attaatagtc ataaaaagca tcgtggttta
     9901 agtcatagtg ctttatgtgc caaaattcgt caaacagaac aaaacgcgct attgcattat
     9961 gaatatcaac acgaagccca acactgataa tcgtcccatt accattggta ttgttgcagg
    10021 agaaatttcg ggagataccc taggtgcagg gttaattaaa gccctaaaac aacgttatcc
    10081 acacgcccgt tttgttggga tttgtggtgc acaaatgatc gccgaaggtg gcgagacatg
    10141 gtttccgcta gaacgtttat cagtgatggg cttagttgaa gtcttggggc gaataaaaga
    10201 attatttgcg attcgagatg aattggttag gcgttttgtt gccgaaaaaa tagatgtgtt
    10261 tataggtatt gatgcacctg attttaactt gcgtgttgca ccacagttaa aacaagcagg
    10321 cattaaaacc gttcattatg tcagcccatc cgtgtgggca tggcggcaag gcaggattca
    10381 tggcattaag gcggcgattg acttaatgtt gtgtttatta ccttttgaga agcagtttta
    10441 tgatcagcat caattagatg cagtatttgt tggccatcct ttagctgata gtctaccctt
    10501 aaaaaacgac actttggccg tacgtcaaca attggggttg gaggcgaata aacaatatgt
    10561 ggcgttgtta ccgggcagtc gtggtggtga ggtgagccgt ttggctgaac cattattcgc
    10621 tgcggctttg ttactaaaaa aacaacatcc acagttagag tttattattc ctgcaattaa
    10681 tgccttacga aaaaagcaga tcagtgaatt gcttgaacaa agcggtttgc aagcaacaat
    10741 ttttgatgat agttatgggg caggggttgg gcgtttggta atggcggctg cggatgtggt
    10801 ggtgttggca tcaggcacag caaccttaga agccatgtta ttaaaaaaac caatggttgt
    10861 ggtttataaa ttacattggc taacctattt aattgtgcgt ttaatggcga aagccaaata
    10921 tattagtttg cctaatttat tagccaatca ggctttagtg ccagagttag tgcaacatgc
    10981 ggtgacacct gaaagtatta gccaagcggt tgagcaatgg ttaaaaccac agcagcagca
    11041 acaaatagtg gcacgtttta cggaattaca ccaacaatta caggcgaatg gcagcgaaac
    11101 agccgcacaa gccattagcc aattaataga gaaacaatga tggaattagc agagatttta
    11161 caaaaatatc aacatattgc tggtgttgat gaagtgggca gagggccttt ggttggtgct
    11221 gtagtaaccg ctgcggtgat tttagatccg aataatccga ttgaaggctt aaatgattcc
    11281 aaaaaactca gtgaaaaaaa gcgtttatta ttagccgaag aaattaaagc caaagcttta
    11341 gcatggagtt taggtcgtgc cgaacgccat gaaattgatg agctcaatat tctaagagca
    11401 acgatgttgg cgatgcagcg tgcggtgaat ggtttaaaaa ttcaccctga ttttgtgttg
    11461 attgatggta ataaatgccc aattttaacc tgcccagcac aagcggtcgt gaaaggtgat
    11521 agtttagtcc ctgctattag cgcagcgagt attttggcaa aagtgactcg tgatgcagaa
    11581 atggacgtat tacatcaaca atatccccaa tatggctttg ctgatca
//
LOCUS       DAOQMB010000031        11486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2181_length_11486_cov_13.991864, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000031 DAOQMB010000000
VERSION     DAOQMB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 11486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11486
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2181_length_11486_cov_13.991864"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1229)
                     /locus_tag="PLY05_02340"
     CDS             complement(72..1229)
                     /locus_tag="PLY05_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004796109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNC02171.1"
                     /translation="MFNFDFYNPTRILFGQQRIADIDQHIPAQAKVLVLYGGGSVKVT
                     GTLKEVLTALGSRHIVEFSGIEPNPTFETLMRAVDIVKSEQIDFLLAVGGGSVIDGTK
                     FVAAASCYQGDVLDILKTRGTVIQAALPIGTVLTLSATGSEMNNGGVITQQSNHTKSA
                     FFSTHTFPQFSVLDPTKTYTLPTRQIANGVVDAFVHITEQYLTYPVNGYVQDRFAEGL
                     LQTLIELGEKALHHPEDYDTRANLMWVATLALNGLIGAGVPQDWATHMIGHELTALHG
                     IDHARTLAIVLPAMLKIRQESKRAKLLQYASRVWQINEGDEQQRIDLAIAKTESFFKG
                     LGVDTRLSAYDLNASHIDAIIEQLIAHKMIKLGEHRDVTPDVCRQVLQLCL"
     gene            1523..2290
                     /locus_tag="PLY05_02345"
     CDS             1523..2290
                     /locus_tag="PLY05_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNC02172.1"
                     /translation="MNLENKVAVVTGGASGLGLATVKAYLAKGASVAIFDLNAEQGAL
                     VAAELGEKALFCQVNVAEEASVQQAIAQTLAKFGAIHVTVNCAGIGSASRTVGKNGAM
                     PLELFSKVIAVNLVGTFNVTRLTAAEMAKNTPDVDGERGVIINTASVAAFDGQIGQVA
                     YSASKGGVVGMTLPLARDLAMLGIRVNTIAPGIFNTPLMNASPDSVKLPLIEMTQFPK
                     RLGYPEEYAFLASHIVENKFMNGETIRLDGAIRMAPR"
     gene            complement(2322..5096)
                     /locus_tag="PLY05_02350"
     CDS             complement(2322..5096)
                     /locus_tag="PLY05_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC02173.1"
                     /translation="MTTISLHSRLLLATLFPTTFLMVIFCIFVVLFRFEDINNLQNET
                     ADILLSKYSYNLTRTPEAQWQEVLEQALSEKNLHSIDIYGSSGIKLVHAGLPSTLNQL
                     PNELVKHLKAQKNPVVYPYRDIFLIPINAPYLDQTNPKWLAIELQSNNFTLARYESVM
                     TISITLFGFVVILLLWVSGNIRYWLNPIQKLIEQIRQINPQQLDKHLDTGATGDLYLL
                     QESINHLLVAVGQEYDELKNLMEQAKHDLDENLITMESRNIELHIAHNHALEGNRAKS
                     AFLANISHELRTPLNSINGFTKLLLKMPLDYKQRDWVESIQKSSEHLLAIIGDVLDFS
                     KIEAGQLSLDKQPIILENIIFEVMDSLAPQAASNGLEQIVFIYEGVPNKVISDSLRLK
                     QVLTNLVNNAIKFVKKNEKGEVIVRVMLEETRGNYHLIRIEVSDKGIGLSKDAKRSLF
                     QAFQQGNPSITREFGGTGLGLAISRNIIKLMDGDIGFNPEQTQGATFWFNFRAGFCAD
                     TDNTPVIHLDNKQIIALESHEKNSQLLRATLSNAGAMITLTKTWSDLIEQLQDTHQVI
                     IFDHHDLDKDKAYEQLKQLRKHFSGLLVMFSHLSDVTKLPDNAYAELHIYTLSKPIRP
                     RHLLSLLAQGLLTARLTQIPVNTTNNTTNSVKIDLHILTVDDHPLNLKLVSTLLEDLG
                     ITVSCAASGMEAIELCSQSKFDLIFMDIQMPQISGLEATQRIRRSEDSLNRNTPIIAL
                     TAHALADEKDNMLKAGMNDYLTKPIDEAQLSYVIEQWTNTHLQPKPQATISSVLEKST
                     VNLISIDWSESLRLSANKSDLAYEMITMLISGLDTAHEKLNKSHQEQNYPALLSHVHH
                     LYGATRYCGVPKLRFLLEQLERLLKDALKKHLEHDLAIQQQRDILLNDIYNEIKQLCT
                     TDINQLINGG"
     gene            5359..6249
                     /gene="cysM"
                     /locus_tag="PLY05_02355"
     CDS             5359..6249
                     /gene="cysM"
                     /locus_tag="PLY05_02355"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HNC02174.1"
                     /translation="MPIMTLEQCVGNTPLVRLQRLGIETGNTLLVKLEGNNPAGSVKD
                     RPALSMIQHAEQRGLIKSGDTLIEATSGNTGIALAMVAAMKGYKMILIMPDHMSQERK
                     DAMAAYGATLIEVSQQQGMEGARDLALKMQTEGKGIVLNQFGNPDNPLAHYQGTGPEI
                     WRDTEGKVTHFISSMGTTGTIMGTSRFLKEQNPNIHIIGLQPMAGAQIAGIRRWPQEY
                     LPTIFEAQRVDQIIDIPQAAAEQTMRDLARFEGICAGVSSGGAVWAMLQLAQQVKNAT
                     MVAIICDRGDRYLSTGLFSR"
     gene            6281..7084
                     /locus_tag="PLY05_02360"
     CDS             6281..7084
                     /locus_tag="PLY05_02360"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC02175.1"
                     /translation="MYKEKATLVFDIETVPDIAKAKQIYNLGNLTNEEAYEALKNMRR
                     QETGGSDFFRHHLHKVVCISVVLRMGDSVRVWSLGDENATEAEIIKRFFTAIDKYDVT
                     LVSWNGGGFDLPVLHYRAMLHEITAPMYWELGDHYKEFRYNNYINRFHMRHVDLMDVL
                     SLYQARATQPLEQIATFLGFPGKLGMDGSQVYDLHQQGQIERIRNYCESDVLNTWLIY
                     LRFQLMRGEIMKPAYQQEITLLKNTLASSDKDHLFDFLDAWEDQEAKYL"
     gene            7081..8415
                     /gene="rlmD"
                     /locus_tag="PLY05_02365"
     CDS             7081..8415
                     /gene="rlmD"
                     /locus_tag="PLY05_02365"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNC02176.1"
                     /translation="MNRKQRERIKQQPPITLEVTKLGHDGRGVAHHDGKVVFVDGALA
                     GEVVEAKVTLIRSKYDEANTVNVIKASSLRVQPPCKHFGVCGGCSLQHIDAQAQIELK
                     QEVLAEQFKHFGQLEPQEWLAPLRHSQAGYRRKARFGVRFVIKKDSLLLGFREKGSNY
                     LAELESCAVMDPRLGQSIMPLRELIGGLAGKDDIPQVEVAAGDDSVALIFRHMQPLVD
                     EDVQALINFCQQRDWQCYLQPSGYDSVHRVYPVDGEDRLHYAMPDFGLTMRFYPLDFT
                     QVNAEINQKMVKLALDLLAPQPHERVLDLFCGLGNFTLPLATRAQYVIGVEGDQAMVE
                     RGYENARHNQLSNVDFYAQDLTADFSQQPWAKEGFDALLIDPPRSGALEVVRYLPNFG
                     AKRIVYVSCNPATLARDAGELAKAGYTLVKAGVMDMFTHTAHVESIALFIKD"
     gene            8496..>10265
                     /locus_tag="PLY05_02370"
     CDS             8496..>10265
                     /locus_tag="PLY05_02370"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNC02177.1"
                     /translation="MVKVREDYPMTADGAVDLELWLQRIAARVELQNPDVLRQACLFA
                     QEADEVYDREHLWSARTSSFLTGLAMADILADLKLDQESLVAAVLYRAVREEKTTLEI
                     IARVFGRDTAQLIDGVLKMATISQLINPNSHNQKFSQSQQQLDNVRKMLVSMIDDVRI
                     ALIKLAERTFAIRELKDANPERQRRVAREIFDIYAPLAHRLGIGHIKWELEDLSFRYL
                     EPEAYKHIARLLDEKRLQRDEYIHNVTKTLRDTLAHADIQAEVSGRAKHIYSIWRKMQ
                     RKKLSFHELYDIRAVRVLVPSVKDCYAALGIVHSLWKHIPKEFDDYIATPKENGYRSL
                     HTAVIGPEGKALEVQIRTNNMHEEAELGVCAHWVYKEGTKGNKDAGYESKIAWLRQVL
                     EWQDDMGETQVDEFADQLSQAIRYERVYVFTKDGHVIDLQAGATPLDFAYSIHTQIGH
                     GCRGAKVNGRIVPLTYKLQTSDQVEILTQKGATPSRDWLLPSLGYLGTSRAQAKVKQW
                     FKQQDRENNITAGREILEKELSRLALNMKMVNLQDYAARFNVKHSDDILAGIGAGDIR
                     PAHIVNVIAEKLTQPQVEVQPQEV"
     assembly_gap    10266..10365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10366..10655
                     /locus_tag="PLY05_02375"
     CDS             <10366..10655
                     /locus_tag="PLY05_02375"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HNC02178.1"
                     /translation="YLHLPLSGGIMGMVLLFAFLLIYGRVPESLAFFTPKFLQHLALY
                     LLPVSAGVITLWPLLQKEGLLIISVMVISTLIPLVLCAWCLDKWLSKQGKF"
     gene            10655..11350
                     /locus_tag="PLY05_02380"
     CDS             10655..11350
                     /locus_tag="PLY05_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HNC02179.1"
                     /translation="MASDLLSTPLVWMTVTLTAYEAGFWLHRRLKEPVLVPPMLLSLT
                     ILVAVLLAADVNYQQYFKANAFIHFLLGTSTVALAVPLYQSLPRLKAAALPLLVVLLI
                     GSVVGAGVALALAQLSGLSHSSVLAFATRAVTTPMALGIAEKIHAPLALASAIVIVSG
                     LVGAIAAEALLKFVRHDMAKGFALGLAAHGVGTARALQISPTAGAFAALGMSLNGILT
                     ALWLPSVIKWVFA"
BASE COUNT         3360 a         2028 c         2476 g         3522 t
ORIGIN      
        1 gctgcgtttt gtgccttgcc tgagtcaatt tttatcagca acgcagctca cacacaaaac
       61 gtcaacagac cctataaaca gagttgtaat acttgacggc aaacatcggg tgtcacatca
      121 cgatgttctc ctaatttaat catcttgtgg gcaatcagtt gttcaataat ggcatcaata
      181 tggctagcat tgaggtcata agcacttaaa cgtgtatcga ctcccaaacc tttaaagaag
      241 ctctcggttt tagcaatcgc taaatcaata cgttgttgtt cgtcgccttc gttaatttgc
      301 caaacacgac tagcatattg cagcaattta gcacgtttgc tttcttgacg aatttttaac
      361 atggcaggca agacaatggc taaggtgcgt gcatggtcaa tgccgtgtaa tgctgtgagc
      421 tcatggccaa tcatatgggt tgcccaatct tgtggcacac ctgcaccaat taaaccattt
      481 aacgctaaag tggcaaccca cattaaattg gcacgtgtat cgtagtcttc agggtgatgg
      541 agggcttttt cacctaattc aatcaaggtt tgtaataaac cttcggcaaa acgatcttgt
      601 acataaccat taacaggata ggtaagatat tgctcggtaa tatgcacaaa cgcatcaaca
      661 acgccattcg caatttgacg agtgggtaaa gtataggttt tggtggggtc taaaaccgaa
      721 aattgtggaa aagtgtgtgt gctaaaaaaa gcggatttgg tgtgattgct ttgttgggta
      781 atcacgccac cattattcat ttctgaacct gttgccgata gcgttaaaac agtgccaatg
      841 ggtaatgcag cttgaataac tgtgccacgg gtttttaaaa tatctaacac atcgccttga
      901 taacaactcg ccgcagccac aaatttagtg ccatcaatca ccgacccacc gccaaccgcc
      961 aataaaaaat caatttgttc cgatttaaca atgtcaacgg ctctcatcag cgtttcaaag
     1021 gttggattag gctcaatgcc tgaaaactca acaatatgtc ttgagcctaa ggcggtaaga
     1081 acttctttga gtgtgcctgt gactttgaca ctaccgccgc catacagcac taaaactttt
     1141 gcttgggcgg gaatatgttg atcaatatca gcaatacgct gttgaccaaa taaaatacgg
     1201 gttgggttgt aaaagtcaaa attaaacatg atatccctcg tcatttttta gcaggttttg
     1261 tgtaaaccgg acaattgctt ggtttggcca ttgtgcctaa aaaaaattat gttataagaa
     1321 cgatgctgcc gcattgcaga tgataagact gacgcatttg atcgaaataa aagtgtcttg
     1381 taaacataaa accgctaaaa atggttgaaa tgtgaacaaa aagcgataac tgcataagtc
     1441 ttcatgatag tagatgttta tcaactaagt ggagggggag caatgcgaca gaaataacac
     1501 tttatgcata ggattataaa cgatgaattt agagaataaa gtagccgttg tcacaggcgg
     1561 tgcatcaggt ttgggcttag caacagtaaa agcctatttg gccaaaggcg catcagtcgc
     1621 catttttgat ttaaatgccg agcaaggtgc tttagttgcc gcagagcttg gcgaaaaagc
     1681 cttattttgc caagtgaatg tggctgaaga agcctcggta caacaagcca ttgcccaaac
     1741 tttagcaaaa tttggtgcaa ttcatgtgac cgtgaattgt gcaggtattg gttcggcaag
     1801 ccgtaccgtt ggtaaaaacg gtgccatgcc attggaactt tttagcaaag tgattgcggt
     1861 taatttagtg gggactttta atgtgacccg tttaactgcc gcagaaatgg ccaaaaatac
     1921 gccagatgtt gatggtgaac gtggtgtgat tattaatact gcctcggttg ctgcatttga
     1981 tggccaaatt ggtcaagtgg cttattctgc ttctaaaggt ggtgtggtgg gtatgacttt
     2041 gccattagct cgtgatttgg cgatgttggg aattcgtgtt aataccattg cacctggtat
     2101 ttttaataca ccattaatga atgcttctcc agacagtgtt aaattgccat tgattgaaat
     2161 gactcaattt cccaagcgtt taggctatcc cgaagaatat gcctttttag cttcgcatat
     2221 tgttgaaaac aaatttatga atggcgaaac cattcgtttg gatggcgcaa ttcgtatggc
     2281 tccacgctaa ttgctaatta agtatatggc ttggcggcta gttagccgcc attaatcaat
     2341 tgattgatgt ctgttgtaca taactgtttt atttcgttat aaatatcatt tagcaaaata
     2401 tcgcgttgtt gttgaatagc caaatcatgc tctaagtgtt tttttagtgc atctttgagc
     2461 aagcgttcta actgttctaa taaaaagcgt aatttaggga caccgcaata gcgtgttgca
     2521 ccatataaat gatgtacatg tgacaacagt gcagggtagt tttgttcttg atgcgattta
     2581 ttgagttttt cgtgtgcggt gtctaaacca gaaatgagca tggtaatcat ttcataggct
     2641 aagtcgcttt tgttggcact gagtcttaag ctttcagacc aatctataga aattagatta
     2701 acggtcgact tttctaaaac agaagatatt gtggcctgtg gtttgggttg caaatgcgtg
     2761 tttgtccatt gttctatcac ataacttaat tgtgcctcat caatgggttt ggtcaaataa
     2821 tcattcatcc cagcttttaa catattgtct ttttcatcgg ctagggcatg agccgttaaa
     2881 gcaataatgg gtgtatttcg atttaaactg tcttccgaac gacgaatacg ttgagtagcc
     2941 tctaaaccag aaatttgcgg catttgaata tccataaaaa tgagatcaaa tttgctttga
     3001 gagcataatt caatagcctc catgccactt gctgcacagc ttaccgtaat acctaaatct
     3061 tctaataacg ttgaaactaa ttttaaattc agaggatgat catcaaccgt cagaatatgt
     3121 aaatctattt tgacagagtt agttgtattg ttggttgtat tcacaggtat ttgtgtcaat
     3181 ctagcagtga gtaatccttg tgctaaaaga cttaatagat gtcttggtct gataggtttg
     3241 gataaagtat aaatatgtaa ttcggcataa gcattatcag gtagtttggt tacgtcagat
     3301 aaatgactaa acataactaa taaacccgaa aaatgtttac gtaactgttt taactgctca
     3361 taggctttgt ctttatctaa atcatgatga tcaaaaataa tcacttgatg ggtatcttgc
     3421 aattgttcaa tgaggtctga ccaagtttta gtgagcgtaa tcattgcgcc agcattagat
     3481 aaagtggctc tcaataattg actatttttt tcgtgagact ctagggcaat aatttgtttg
     3541 ttatcaagat gaatgacagg tgtattatct gtgtctgcac aaaaacctgc tctaaaatta
     3601 aaccaaaatg ttgcaccttg ggtttgctct gggttaaagc caatatcacc atccattaat
     3661 ttgataatat tccgcgagat agctaagcct aaacctgtac caccaaattc acgggtgatg
     3721 gaaggattgc cttgttgaaa ggcttgaaat aaagagcgtt tagcatcttt acttaaaccg
     3781 atgcctttat cactcacttc aatacgaatt aaatgataat taccccgagt ttcttcaagc
     3841 ataaccctaa caatcacttc acctttttca tttttcttaa caaatttaat agcgttattg
     3901 actaagttag tgaggacttg tttgaggcgt aaactgtcgc taataacttt attgggaaca
     3961 ccctcataaa taaacactat ttgctccaag ccattgctag cggcttgtgg tgctaaacta
     4021 tccatgactt caaaaataat attttccaaa ataatgggtt gtttatctaa agataattgg
     4081 ccagcttcga ttttggaaaa gtctaaaaca tcaccaataa tggctaacaa atgttcggat
     4141 gatttttgaa tgctttcaac ccagtcgcgt tgtttatagt ctaagggcat ttttaacagt
     4201 aatttagtaa aaccattgat actatttaga ggcgtgcgta attcatgact aatgttggct
     4261 aaaaatgcgg atttagctct atttccttct agggcgtgat tatgtgcaat gtggagttca
     4321 atattgcgtg actccattgt gattaaattt tcatctaaat catgtttggc ctgttccatt
     4381 aaatttttaa gttcatcgta ttcctgacca acagcaacta gcaaatggtt aatactttct
     4441 tgtagcaaat ataaatcgcc tgttgcgcct gtatcgaggt gtttatcgag ttgttgagga
     4501 ttgatttgtc gaatttgttc aatcaatttt tggataggat taagccaata acgaatattg
     4561 ccactcaccc ataacaataa aatgaccaca aaaccaaata aggtaattga aatagtcatc
     4621 accgattcat aacgagctaa agtaaagtta ttgctttgta attcaatggc taaccattta
     4681 ggattggttt ggtctagata tggcgcatta attgggatta aaaatatatc gcgataggga
     4741 tatacaacag ggtttttttg agcttttaaa tgtttgacta attcattagg cagttgattg
     4801 agtgttgagg gtaatcctgc atgaaccaac tttatgccag aagagccata aatatctata
     4861 ctatgtaggt ttttttcgct gagagcttgt tctaaaactt cttgccattg tgcttcaggc
     4921 gttctggtta agttatagct atatttactt aataaaatgt ctgcagtttc gttttgtaaa
     4981 ttattaatat cttcaaaacg aaaaaggaca acaaaaatac aaaaaatgac cattaaaaaa
     5041 gtggttggaa ataaagtagc tagcaataaa cgcgagtgta aactgattgt ggtcatgaca
     5101 ggtggccatg taaaaaaggt gaatttttta aataaattaa atcttaacag actgtagggg
     5161 ctgttgacat ttggattgtg gttgcgaaag aagctatttt ttcaggtttt agcgataatc
     5221 acagaaaaaa gataaaaaaa gtgctgcttt taacttaagc tgttttacaa tgcgcggcca
     5281 aaaagtaagg acttatgcgg tttatcaaat ttgccacagt atcattggat taaatttttc
     5341 tcaaaagagg aattagcaat gcctattatg accttagagc aatgtgtagg aaatacaccg
     5401 ttagtacgct tgcagcgttt aggtatagaa acaggtaata cgcttttagt taaattagaa
     5461 ggcaataatc cagcaggctc ggtcaaagat cgtccagcat taagtatgat tcaacatgcc
     5521 gaacaaagag gtttaattaa atcaggtgat actttaattg aagcaaccag tggcaataca
     5581 ggtattgctt tagccatggt ggctgccatg aaaggttata aaatgatttt aattatgcct
     5641 gaccatatga gccaagagcg taaagacgcg atggcggctt atggtgcaac attaattgaa
     5701 gtttcacagc aacaaggcat ggaaggcgca agagatttag ccttaaaaat gcaaacagaa
     5761 ggcaaaggca ttgttttaaa tcaatttggt aatcccgata atccacttgc ccattatcaa
     5821 ggaacaggcc cagaaatatg gcgagataca gaaggaaaag tcactcactt tattagctcg
     5881 atgggcacaa caggtacaat tatgggaact tctcgatttt tgaaagagca aaatcccaat
     5941 atacacatta ttggtttgca acccatggca ggtgcgcaaa tcgcaggtat tagacgttgg
     6001 ccgcaagagt atttgccaac catttttgaa gcacaacgtg ttgaccaaat tattgatatt
     6061 ccacaagctg ctgccgaaca aacgatgcgt gatttagccc gttttgaggg catttgtgca
     6121 ggtgtttcat caggcggagc ggtttgggct atgttacaat tagctcagca agttaaaaat
     6181 gcgacgatgg ttgcaattat ttgtgatcgt ggagatcgtt atttatcaac gggattattt
     6241 agccgttaat ttattattct tagaataggg agcgcgagtt atgtataaag aaaaagcgac
     6301 tttggttttt gatattgaaa cagtacctga tattgccaaa gccaaacaaa tctataactt
     6361 aggtaattta acaaacgaag aagcctatga agccctaaaa aatatgcgtc gccaagaaac
     6421 gggtggttct gattttttta gacatcattt gcataaagtt gtgtgtattt ctgtggtatt
     6481 acgcatggga gacagcgttc gtgtgtggtc attaggtgat gaaaatgcca ctgaagctga
     6541 aattatcaag cgttttttta ccgccattga taaatatgat gtcactcttg tttcttggaa
     6601 tggtggtggt tttgacttgc ctgttttgca ttatcgtgcg atgttgcacg aaatcaccgc
     6661 cccaatgtat tgggagttag gcgatcatta taaagaattt agatacaaca attacatcaa
     6721 tcgttttcat atgcgccatg tcgatttgat ggatgttttg tcattgtatc aagcacgtgc
     6781 aacacagccc ttagaacaaa ttgccacttt tttaggcttt ccaggtaaat taggcatgga
     6841 tggcagccaa gtgtatgact tacatcagca aggtcaaatt gagcgcatcc gcaattattg
     6901 cgaaagtgat gttttaaata catggttgat ttatttacgt tttcaattaa tgcgtggcga
     6961 aattatgaaa cccgcatatc agcaagaaat tactttatta aaaaacacct tagccagcag
     7021 tgataaagac catttgtttg actttttaga tgcttgggaa gatcaagaag cgaaatattt
     7081 atgaacagaa agcaacgtga acgaattaaa cagcaaccac caattacttt agaagtgaca
     7141 aagttgggac atgatgggcg aggtgttgct catcatgatg gaaaagtggt ctttgttgat
     7201 ggtgctttag caggcgaagt ggttgaagcc aaagtcacgt taattcgtag taaatatgat
     7261 gaagcaaaca ctgttaatgt aattaaggct tcatccttaa gagtacaacc gccatgcaaa
     7321 cattttggtg tgtgtggtgg ttgctctttg caacacattg atgctcaggc acaaattgag
     7381 ctgaagcaag aagtgttagc tgagcaattt aaacattttg gacaacttga gccacaagaa
     7441 tggttagctc ctttgcgcca cagtcaagca ggctatcgtc gtaaagcgcg ttttggcgtg
     7501 cgttttgtca ttaaaaaaga tagcttgtta ttaggtttta gagagaaagg ctcaaactat
     7561 ttagctgaat tagaaagctg tgcggtgatg gatccccgtt taggccaatc gattatgcca
     7621 ttacgcgagt taattggtgg tttggcaggc aaagatgata ttccacaagt tgaagtcgcc
     7681 gcaggtgatg acagtgtggc gttgattttt cggcatatgc agcctttagt tgatgaagat
     7741 gtacaagcat tgattaattt ctgtcaacaa cgtgattggc aatgttactt gcagccaagt
     7801 ggttatgaca gtgtccatcg tgtttatcca gtcgatggtg aagatcgttt gcattacgcc
     7861 atgcctgatt ttggtttaac gatgcgtttt tatcccttag actttaccca agttaatgct
     7921 gaaattaacc aaaaaatggt gaagttggct ttagacttat tagcaccgca gccacatgaa
     7981 cgggtgttag atttattttg tggtttaggc aactttactt tgcccttagc aacacgcgct
     8041 caatatgtga ttggtgttga aggcgatcaa gcaatggttg aacgtggtta tgaaaatgcc
     8101 cgtcataatc aactcagcaa tgtcgatttt tatgctcaag atttaaccgc tgatttttcc
     8161 caacaaccgt gggctaaaga aggttttgat gcgttattaa ttgacccgcc acgttcaggt
     8221 gcgttggaag tggtacgata tttgcctaat tttggagcaa aacgtattgt ctatgtgtcc
     8281 tgtaatcctg caaccttggc acgagatgct ggcgaattgg ctaaagcagg ctatacctta
     8341 gtcaaggcag gggtaatgga tatgtttacc catacagcgc acgtagaatc tattgcatta
     8401 tttattaaag actaagtact aaattttttc gtacactgag cgaagttgaa atggtttcga
     8461 ctccgctcaa gcaccctagt tttagagcgg taattatggt taaagtacgc gaagactatc
     8521 ccatgactgc cgatggtgca gtcgacctag agttgtggtt gcagcgtatt gccgcacgag
     8581 tcgaactaca aaaccctgat gtgctacggc aagcgtgttt atttgctcaa gaagccgatg
     8641 aagtctatga ccgtgagcat ttatggagtg ccaggaccag tagcttttta acaggtttgg
     8701 cgatggctga tattttggct gatttaaaat tagaccaaga atcactcgtg gctgctgtgt
     8761 tatatcgtgc tgttcgtgaa gaaaaaacaa cgctagagat tatcgctcgt gtttttggcc
     8821 gtgatactgc acagttgatt gatggcgtgt taaaaatggc gaccatcagc caattgatta
     8881 accccaatag ccataatcaa aaatttagtc aatcacaaca acaactggat aacgtccgta
     8941 aaatgttggt gtcgatgatt gatgatgtac gtattgcctt aatcaaattg gcagaacgta
     9001 cttttgccat tcgagaatta aaagatgcca atcctgagcg gcaaagacgt gttgctcgag
     9061 aaatttttga tatttatgcc cctttagccc atcgacttgg cattggccat attaaatggg
     9121 aactcgaaga tttatcgttt agatatttag aacccgaagc ctataaacac attgctcgac
     9181 tcttagatga aaagcgtttg caacgtgatg aatatattca caatgtcact aaaactttgc
     9241 gtgatacctt agcacatgcc gatattcagg cagaggtgtc agggagagca aaacacattt
     9301 attcgatttg gcgcaaaatg cagcgcaaaa agctcagttt ccatgagtta tacgatattc
     9361 gtgcggtgcg tgtgttggtg ccatcagtca aagattgtta tgcagcctta ggtattgtcc
     9421 attctttatg gaaacatatt cccaaggaat ttgatgatta tattgccacc cctaaagaaa
     9481 atggttatcg ctccttacat acagcggtga ttggccctga gggtaaagct ttagaagtgc
     9541 agattcgtac caacaatatg cacgaagaag ctgaattagg ggtctgtgcg cattgggttt
     9601 ataaagaggg cacaaaaggc aacaaagacg caggttacga aagtaaaatt gcatggttac
     9661 gtcaggtctt agaatggcaa gatgatatgg gggaaactca agttgatgag tttgccgatc
     9721 aattaagcca agcaattcgt tatgaacgtg tatatgtgtt tactaaagac ggccatgtca
     9781 ttgatttaca agcaggcgca acacccttag attttgctta tagtattcat acccaaattg
     9841 gtcatggttg tcgtggggca aaggtaaatg gccgtattgt gccgttaacc tataaattac
     9901 aaaccagtga tcaagttgaa attcttaccc aaaaaggcgc aaccccaagt cgagattggt
     9961 tgttaccaag tttaggttat ttgggtactt cacgtgccca agcaaaagta aagcagtggt
    10021 ttaagcaaca agaccgtgaa aataatatta cggctggtcg tgagatttta gaaaaagaac
    10081 tttcccgttt agctctcaac atgaaaatgg tgaatctgca agattatgca gcgcgtttta
    10141 atgttaaaca tagcgatgat attttagcgg gaattggtgc gggtgacatt cgtcctgcac
    10201 atattgtcaa cgtgattgcc gaaaaactca cacaaccaca agttgaagtg caaccacaag
    10261 aagtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattat ctccatttgc
    10381 cgttatcggg tggcattatg gggatggtat tgttgtttgc ttttttatta atttatggtc
    10441 gtgttcctga gagtttagct ttttttaccc ccaaattttt acagcattta gcgttatatt
    10501 tattgcccgt ttctgcgggc gtaattactt tgtggccact gttacaaaag gaaggattgc
    10561 ttattattag cgtcatggtg atttcaaccc ttattccttt agtattgtgt gcatggtgct
    10621 tagataaatg gctaagcaaa caaggtaaat tttaatggct agtgacctcc taagtacgcc
    10681 tctagtgtgg atgacagtaa ctttaaccgc ctatgaagca ggtttttggt tgcatcgccg
    10741 tttaaaagag ccagtcttag taccgcctat gttgttgtca ttaactattt tggttgcggt
    10801 gttattagca gcagatgtga attatcagca atattttaaa gccaatgctt ttattcattt
    10861 tttattgggc acctcaactg ttgccttggc tgtgccttta tatcaatctt taccacgttt
    10921 aaaagccgca gctttgccgt tattagtcgt gttgttgatt ggttcagtgg ttggtgcagg
    10981 tgtggcctta gccttagcgc aattatcggg tttatcacat agtagcgttt tagcatttgc
    11041 cacaagggca gtgaccacac caatggcctt gggtattgcc gaaaaaattc atgcgccatt
    11101 agccttagcg tctgccattg tcattgtttc gggtttagtg ggggcaattg ctgccgaagc
    11161 cttattaaag tttgtgcgtc atgatatggc taaaggcttt gctttgggat tggcagcgca
    11221 tggtgtgggg acggcacgtg ccttacaaat tagcccaacc gcaggggcgt ttgcggcatt
    11281 gggcatgagt ttaaatggta ttttgaccgc tttatggttg ccgagcgtga ttaaatgggt
    11341 ttttgcctaa ttaaaattgt tcagaaaatt agcgtaaatg tcgaagtttt cacgacttac
    11401 tgaaaaaata cctgtaaatc actgcctaaa ttacagaaag atgatattat taggcagccg
    11461 agtttttact cggttttttt gtgtct
//
LOCUS       DAOQMB010000032        11195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2256_length_11195_cov_14.295063, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000032 DAOQMB010000000
VERSION     DAOQMB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11195
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2256_length_11195_cov_14.295063"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            204..335
                     /locus_tag="PLY05_02385"
     CDS             204..335
                     /locus_tag="PLY05_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02180.1"
                     /translation="MAWRSMFEAWMPSSAPQMVLLTFAETKVRPAAGNDKTEAVQYI"
     gene            374..2050
                     /locus_tag="PLY05_02390"
     CDS             374..2050
                     /locus_tag="PLY05_02390"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: protein motif:HMM:NF008594.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNC02181.1"
                     /translation="MNAPYSPSPDYLADTHEIHNVSRELVNYNMYLQDVALQEALHRE
                     GGTLAEQELIEFGSLTGSADYLELGHLANKYPPEFDTHDRFGNRVDLAKFHPAYHQLM
                     KTAIEQGLHASPWTDNRAGAHVIRAAKNILQGQVEAGHGCPITMTFAAVPSIRLQPDL
                     AKTWLPLITSRIYDPRNVPIDQKQGVTIGMGMTEKQGGSDVRANTTKAYAVGAEGGGQ
                     LYKIVGHKFFLSAPMCDAFLVLAQTQRGLSCFLVPRWQSNGQKNPLQVLRLKRKMGNV
                     SNASSEVEFRGAEGWMVGEEGRGVRTIIEMVSMTRFDCMVGSSAGQRMAVSQALHHCS
                     IRAAFGKRLIEQPLMQNVLADLILEYEGSLAFSMRMAYALDHRDDTHEDLLVRLGTAV
                     GKYWICKRTPNHAYEAMECMGGSGVMEDSIFPRLYREAPINAIWEGSGNVQCLDVLRA
                     MYKTPAVVHAFFAEIGKAQAKNALLDAFVSQLQQDLQQLADFEYRARDLVDRLALAFQ
                     AALLLRHAPDYVSHAFCASRLGQQGQHNYGCLPKNCDLEAIINRAWPQMV"
     gene            complement(2106..>2501)
                     /locus_tag="PLY05_02395"
     CDS             complement(2106..>2501)
                     /locus_tag="PLY05_02395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC02182.1"
                     /translation="MRQRKQGRIMNIASIAAFQSCPRFAAYAATKAYVLSLSEALAAE
                     EKANGIKITAVCPGATATAFHDVAGNNGTFLTKMMDSAEDVAASAIKSLNAGKPVIVT
                     GAMNKPLPFFNRLLPRQCVVWMAGKLAAT"
     assembly_gap    2502..2601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2740..3336
                     /locus_tag="PLY05_02400"
     CDS             2740..3336
                     /locus_tag="PLY05_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNC02183.1"
                     /translation="MSTAAQLDFISEQEYLAGEQISELKYEYIDGYVYAMSGASNNHN
                     LIAGNVFATLANHLKKKPCRPYISDMKVKIGSRYFYPDVLVDCSNLTGDSYYTEQPII
                     LVEVLSKSTRRMDETTKRMTYMQIPSLQEYILIEQDFVDVEIIRRETGWLSEHYFLGD
                     ELTLSSIGLTLTVEDIYERVQNNDVKEWLESQQQNLDR"
     gene            complement(3381..5546)
                     /locus_tag="PLY05_02405"
     CDS             complement(3381..5546)
                     /locus_tag="PLY05_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004775422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNC02184.1"
                     /translation="MIGMHWAEDADGIVTLTMDDPTSEANIMNEVFEKALTQTVAKLK
                     EEKARIKGIIVTSAKKTFFAGGDLTALIQAKPEHAPIFEQKVTELKALLRALETLGKP
                     VVAAMNGTALGGGLEIALSCHHRIALNNPKAEFGLPEVSLGLLPGAGGVIRTVRMLGI
                     QNALMNVLLKGDRMKVAKAKSVGIIDDIAETPEEMFAKAKAWIAANPNAQQPWDKQGY
                     KIPGGSPSTPSFAMNLPAFPANLQKQIKGANYPAPKAILCTAIEGAQVDFDNALKIEG
                     RYFTSLACGKESKNFIKAFFFDLQKINKGASRPSKETYPVWKSKKVGVLGAGMMGQGI
                     AYVSAMSGIEVVLLDMSLEAAERGKGYTQKLLDKRVSKGRMTKEAAAEKLALIKATND
                     FADLAGCDLVIEAVFESREIKADVTKKVEAVCDAELVFASNTSTLPITGLATASQRPQ
                     NFIGLHFFSPVDKMPLVEIICGEKTNDETLAKAFDYVLQIKKTPIVVNDSRGFFTSRV
                     FGTFCMEGVNMLAEGFNPLSIEQAAAQAGMPVGPLAILDEVNMELTRKIRKQTQVDLA
                     AEGKEIPMEPAFGLIDKMCDDFKRPGKKDGAGFYEYPADGKKRIWSGLSAFVTDDKKQ
                     PSVAQFTELKERLLYRQSIEAARCYQEGVLRSVADANIGSIFGIGMAPWTGGQLQYIN
                     YIGVAEFVKRADELSAKFGERFAPPQLLRDMAAKGETFQ"
     assembly_gap    5568..5667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5668..6878)
                     /locus_tag="PLY05_02410"
     CDS             complement(<5668..6878)
                     /locus_tag="PLY05_02410"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011182661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC02185.1"
                     /translation="MSTEAFIYDAVRTPRGKGKKDGSLHTIKPIDLVVGLMDALAERN
                     GQGIKQLADDVVLGCVTPVGDQGADIAKTAALAAGLADTVAGVQINRFCASGLEAVNM
                     AAMKVRSGFEDLVIAGGVESMSRIPMASDGGAWAMDPATSLATGFVPQGIGADLIATI
                     EGFSRTDIDSFALQSQKRAAAAQASGHFDKSVVPVKDINGLTVLAKDEFIRGNATLEG
                     LGALQPSFMMMGEMGGFNAVALQKYHWVESINHVHTAGNSSGIVDGAALMLIGNEAAG
                     KKLGLKPRARFVATALSGADPTIMLTGPAPAAYKALEKAGLTVADIDLWEINEAFAAV
                     ALRFMKDMGIKDDIVNVNGGAIAMGHPLGATGCMILSTLLDELERRNLKRGLATLCVG
                     GGMGIATIIERV"
     gene            7337..9964
                     /gene="alaS"
                     /locus_tag="PLY05_02415"
     CDS             7337..9964
                     /gene="alaS"
                     /locus_tag="PLY05_02415"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNC02186.1"
                     /translation="MRSSADIREAFLSFFESKGHTRVASSSLIPANDPTLLFTNAGMN
                     QFKDCFLGLDKRAYTRAVSSQKCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYF
                     KHDAIAYAWEFLTSPAWLNIPSDKLWVTVYATDDEAFQLWQDVAGVPAERIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGADIWGGLPGSAEEDGDRYIEIWNNVFMQF
                     NRTADGVLHPLPAPSVDTGMGLERISAVMQHVHSNYEIDLFQHLLQAAADAIGCSNEQ
                     QASLKVVADHARSCSFLVADGVVPSNEGRGYVLRRIIRRACRHGNKLGATQPFFYKMV
                     AALAQVMGAAYPELVERQAFIEKVLKQEEEQFARTLEQGLKILEQDLVNLQGTVISGD
                     VVFKLYDTYGFPMDLTADIARERHLSIDEAGFNACMDAQRERSQKAGKFNFDYNSLLK
                     VDVDTVFTGYDSTCSEGKVVAMFIGDEAVNHVNEGQKAVVVLDKTAFYAESGGQVGDT
                     GVLSVGHTRFEVQNTTKVGNAFLHHGIVTQGQLLVNDTVEGQVDNTLRANTARNHSAT
                     HLLHAALRQTLGTHVQQKGSLVCADYLRFDFSHTEGVTAEQISQIEALVNGQILANST
                     VATEVTDIDTAKAKGAMALFGEKYGDTVRVLSMGTDNFSVELCGGTHVSRTGDIGLLK
                     ITSEGGVAAGIRRIEAKTGSGALAWLAEGEQALAHIGQLVKGSRQAVADKVQQLAERN
                     RDLEKEIERLKAKLASSASNDLLSQVQDINGVKLLAVKLEGADSKSLRTTLDQLKNKL
                     ASGVIVLATVEGDKVTVIAGVTADLTSRFKAGDLVQHISTQLGGKGGGRPDMAQGGGT
                     NVAALPQALNSVSLWLQQK"
     gene            9977..11194
                     /locus_tag="PLY05_02420"
     CDS             9977..11194
                     /locus_tag="PLY05_02420"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNC02187.1"
                     /translation="MALIVQKYGGTSVGSPERIQAVAERVERWHKQGHDIVVVVSAMS
                     GETNRLLALAKAITDEPDPRELDQMVSTGEQVTIALLAMALQKRGLGAKSMTGAQVAI
                     RTDSAFTKARIESIDDAAMRAELAKRNVIIVAGFQGIDAQGNITTLGRGGSDTTGVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPQARRLEKITFEEMLEMASLGSKVLQIRSVEF
                     AGKYQVPLRVLSSFAEGEGTLITTDSEDTMEQPVISGIAFNRDEAKLTIRGVPDTPGI
                     AYRILGPIGDANIEIDMIVQNISADNTTDFTFTVHRNEFRKALPILENIAKEIGAREV
                     VGDDKIAKVSLVGVGMRSHAGVASKMFGTLAKENINIQMISTSEIKISVVVEEKYMEL
                     AVRALHTAFGLDA"
BASE COUNT         3188 a         2392 c         2297 g         3118 t
ORIGIN      
        1 actgttgccc cattgcgggt taactatcac cgtgaacatc atttaatggc aggtgtccct
       61 tattttaatt tacctgtgat gcaccacatg ttgttagcca aaggggtgat tgataatcca
      121 cctaactatg tggatgtgtt gaagattgtg agtgctaaaa cggtatgaca aatgtctgtt
      181 gcctaacagt ggggatttca gggatggcgt ggcgcagcat gttcgaggca tggatgccga
      241 gttctgcgcc acaaatggtt ttgcttactt ttgccgaaac aaaagtaagg cctgccgcag
      301 gcaatgataa aactgaagcc gttcaatata tttaatctta actcatttaa aacgataaat
      361 actggagtcc gccatgaatg ccccttactc cccaagccct gattatcttg ctgacactca
      421 cgaaatccat aacgtcagcc gtgaattagt taattacaac atgtatttac aagatgttgc
      481 gctacaagaa gccctacatc gtgaaggtgg tacattggct gagcaagaat tgattgagtt
      541 tggttcatta acaggttctg ccgattattt agaacttggc cacttagcca ataaatatcc
      601 acccgaattt gatacccacg accgttttgg taatcgtgtt gatttagcca aatttcatcc
      661 tgcttatcat caattgatga aaacagcgat tgaacagggt ttacacgcct caccgtggac
      721 ggataatcgt gcaggtgcac acgtaattcg tgccgcaaaa aacatcttac aaggccaagt
      781 ggaagcgggt catggctgcc cgattaccat gacctttgct gctgtacctt ccattcgttt
      841 gcaaccagac ttagccaaaa cgtggctgcc attaatcacc tcgcgtattt atgacccgcg
      901 caacgtaccc attgaccaaa aacaaggtgt aacgattggt atggggatga ctgaaaaaca
      961 aggcggttct gatgtacgag ccaataccac caaagcctat gctgttgggg cggaaggtgg
     1021 tggccaactg tataaaatcg ttggtcataa attctttttg tctgcaccca tgtgtgatgc
     1081 ctttttggtg ttagcacaaa ctcaacgtgg tttgtcatgc tttttagtac cacgctggca
     1141 aagcaatggc caaaaaaatc cactgcaagt gcttagacta aaacgcaaaa tgggtaatgt
     1201 ctctaatgcg tcgagcgaag ttgaatttcg gggtgctgag ggctggatgg ttggcgaaga
     1261 aggtcgtggt gtgcgtacca ttattgaaat ggttagcatg acccgttttg attgtatggt
     1321 aggttcaagt gctggacaac gtatggcggt ttcccaagcc ttacaccact gctctattcg
     1381 tgctgcattt ggtaaacgct taattgaaca accgttaatg caaaatgtgc ttgccgattt
     1441 aattttggaa tatgaaggct cattggcctt tagtatgcgc atggcttatg ccctagacca
     1501 tcgtgatgat acgcatgaag atttattggt gagattaggc acagccgtgg gtaaatactg
     1561 gatttgtaaa cgcacaccta atcacgctta tgaagccatg gaatgtatgg gtggtagtgg
     1621 tgtaatggaa gatagtattt tcccccgttt ataccgtgaa gcgccgatta atgcgatttg
     1681 ggaaggcagt ggcaatgtcc aatgtttaga tgttttgcgc gccatgtaca aaacacctgc
     1741 ggtggtacac gccttttttg ctgaaattgg caaagcgcaa gctaaaaacg cactgttaga
     1801 cgcttttgtc agccagttgc aacaagactt acaacaatta gccgattttg aatatcgggc
     1861 gcgtgatttg gttgaccgtt tggccttagc gtttcaagct gcattactct tacgccatgc
     1921 gcctgattat gtcagccatg cgttttgtgc ctcacgttta ggccaacaag gccaacataa
     1981 ttatggttgt ttgccaaaaa attgtgattt agaggcgatt attaacagag catggccgca
     2041 aatggtgtaa aaaatgataa ctccctcctt tattaattaa aggagggtta gaaagctgta
     2101 aaaacttaag tcgctgccaa ttttcctgcc atccacacca cacattgacg tggtaataaa
     2161 cgattaaaaa atggtaaagg tttattcatc gcgcctgtca caatcacagg tttacccgca
     2221 tttaacgatt tgatagcact cgctgcgaca tcttcggcag aatccatcat tttagttaaa
     2281 aatgtaccat tattacctgc aacatcatgg aaagccgttg cggttgcacc ggggcaaact
     2341 gcggtaattt taatgccatt ggctttttct tcggcagcta aagcttcact taacgataac
     2401 acataagctt tggtggcagc ataagcggca aaacgtgggc aactttgaaa agcagcgata
     2461 gaagcaatat tcataatacg accttgtttg cgttgacgca tnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn naattcgata attatcaaat tataaaaaca tcaaactgag
     2641 aaatatttgg cattatttta tttttgccgt tcttcaccat ctagacagct tatttttgct
     2701 aaaatcagcc aatggtgatt agccaagcgg agtttaacca tgtccacagc agcacaactt
     2761 gattttatta gcgaacaaga atacttggca ggcgaacaaa tttctgagct caaatatgaa
     2821 tatatagatg gttatgtata tgccatgtct ggggcaagta ataatcataa tcttattgca
     2881 ggtaatgttt ttgctacatt ggccaatcat ttaaaaaaga aaccctgtcg cccttatatc
     2941 tctgatatga aagtcaaaat tggcagtaga tatttttatc ctgatgtatt ggttgattgc
     3001 tccaacttaa caggtgacag ttactatact gaacaaccga ttattttagt tgaagtatta
     3061 tccaaatcga cacgacgtat ggatgaaacc actaaacgta tgacttatat gcaaattccc
     3121 agcttgcaag aatacatatt aattgaacaa gattttgtcg atgtagaaat tattagacgc
     3181 gaaacaggtt ggttatcaga acattatttt ttaggtgatg aactgactct ttcatccatt
     3241 gggctaacgt tgactgttga agatatttat gaacgggtac aaaataacga tgtaaaagaa
     3301 tggttagaaa gccaacagca aaatcttgac cgataaaaaa ggcaaacaga tgtttgcctt
     3361 ttttcatcta cagttaaaac ttattgaaaa gtctcacctt tggcagccat atcacgcaat
     3421 aattgaggtg gcgcaaaacg ctcaccaaat tttgcggata actcatcggc acgtttaaca
     3481 aactcagcca caccaatata attaatatat tgcaattggc cgcctgtcca cggtgccata
     3541 ccaataccaa aaatcgaacc gatattggca tcagccacag agcgtaatac gccttcttga
     3601 taacaacgtg ctgcttcaat ggattggcga tataacaaac gctcttttaa ctcagtaaac
     3661 tgagcaactg aaggttgttt tttatcatca gtgacaaagg cagataaacc agaccagatg
     3721 cgctttttgc catcagcagg atattcatag aagcctgcac cgtctttttt accaggacgc
     3781 ttaaagtcat cacacatttt gtcaattaag ccaaacgcag gttccattgg aatttctttg
     3841 ccttccgccg ctaaatccac ttgtgtttgt ttgcgaattt tacgggttaa ttccatatta
     3901 acttcgtcaa gaatcgctaa tgggccaaca ggcatacctg cttgagcagc cgcttgttcg
     3961 attgacaaag gattaaaacc ttcggccaac atattgacac cttccataca gaaagtacca
     4021 aacacgcgag aagtgaagaa gccacggctg tcattaacca caatgggcgt tttcttaatt
     4081 tgtaatacgt aatcaaaagc tttggctaag gtttcatcat tggttttttc accacagatg
     4141 atttcaacta acggcatttt gtcgactggc gagaagaaat gtaaaccaat aaagttttgt
     4201 ggacgctgag aagcggttgc taaacctgta attggcaatg ttgaggtgtt agaagcaaag
     4261 actaattctg catcacacac cgcttcaact ttcttagtaa catcggcttt aatttcacgg
     4321 ctttcaaata ccgcttcaat caccaaatca caacctgcta aatcggcaaa gtcattggtg
     4381 gctttaatta aggctaattt ttcagcagcc gcttctttgg tcatacgacc tttagagaca
     4441 cgcttatcga gtaatttttg ggtgtaaccc ttaccacgtt cagccgcttc taaagacata
     4501 tctaataaaa cgacttcaat gccagacata gcagagacat aagcaatgcc ctgacccatc
     4561 atgcctgcgc ctaatacgcc gacttttttg cttttccaca caggataagt ttctttagaa
     4621 ggacggctag cacctttatt gattttttgt aaatcaaaga agaacgcttt aataaagttt
     4681 ttcgactctt tgccacaggc taagctagta aaataacgac cttcaatttt taaggcatta
     4741 tcaaagtcaa cttgtgcgcc ttcaatggcg gtacacaaaa tcgctttagg agcaggataa
     4801 tttgcacctt taatttgctt ttgtaagttc gcagggaaag cgggcaaatt catcgcaaag
     4861 ctgggtgtgc tgggtgagcc accaggaatt ttgtagcctt gtttgtccca aggctgttgc
     4921 gcgtttggat tggctgcaat ccacgctttg gctttagcaa acatttcttc gggcgtttca
     4981 gcgatgtcat caataatgcc aacacttttg gcttttgcca ctttcatgcg gtcgcctttt
     5041 aataagacgt tcattaaggc attttgaata cctaacatac gcacggtacg aatcacacca
     5101 cctgcccctg gtaataaacc taaagacact tcgggtaaac caaattcggc ttttggattg
     5161 tttaaggcaa tacggtgatg acagcttaat gcaatctcta aaccaccacc caaggctgtg
     5221 ccattcattg cagcaacaac aggcttacct aaagtttcta aagcacgtaa taaggctttt
     5281 aattctgtga ctttttgttc aaaaatcggt gcgtgttcag gttttgcttg aattaaggcc
     5341 gttaaatcac cacctgcgaa gaaggttttt ttcgcagaag tgacaataat acctttaata
     5401 cgtgcttttt cttctttgag tttggcgaca gtttgtgtca atgccttctc aaagacttca
     5461 ttcataatat ttgcttcgct agttgggtca tccatcgtta aggtgacaat gccatcagcg
     5521 tcttcagccc agtgcatacc aataatatca gtcatttact atactccnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnaca cgctcaatga tggttgcaat gcccataccg
     5701 ccaccgacac acaaggtagc aaggccacgt tttaagttac gacgctctaa ctcatccaat
     5761 aaagtgctta aaatcataca acctgttgca ccgagcggat gtcccatggc aatcgcacca
     5821 ccgttgacgt taacgatgtc atctttaatg cccatatctt tcataaagcg taaggctact
     5881 gccgcaaaag cttcgttaat ttcccacaaa tcaatatcag caaccgttaa accagctttt
     5941 tctaaagctt tatacgctgc tggtgctggg cctgttaaca taatggttgg gtcagcaccc
     6001 gataatgcag tggcgacaaa acgggcacgt ggttttaaac ctaatttttt gcctgctgct
     6061 tcgttaccaa tcaacatcaa cgctgcacca tcaacaatac ctgatgaatt accagcggta
     6121 tgaacgtgat taatgctttc tacccaatga tatttttgta aggcaaccgc attaaagccg
     6181 cccatttcac ccatcatcat aaacgaaggt tgcaatgcac ctaaaccttc taaggtcgca
     6241 ttaccacgaa taaattcgtc tttggctaat accgttaaac cattaatatc tttgacaggc
     6301 acaacagatt tatcgaaatg accacttgct tgggcagcag ctgcacgctt ttgcgactgt
     6361 aacgcaaagc tatcaatatc tgtacgtgaa aaaccttcga tcgtggcaat taaatcagca
     6421 ccaatacctt gaggcacaaa acctgtggct aagctagttg caggatccat tgcccatgca
     6481 ccaccatcag aagccattgg aatacgtgac atagactcta cgccaccagc aatgactaaa
     6541 tcttcaaagc ctgaacgtac tttcatcgct gccatattca ctgcttctaa gccagaagca
     6601 cagaaacggt taatttgcac gcctgcaaca gtgtctgcta aaccagcggc taaggcggca
     6661 gttttagcaa tatcagcacc ttgatcgccc acaggtgtca cacaacccaa gaccacatca
     6721 tcagccaact gtttaatacc ttggccatta cgctctgcta aggcatccat taaaccgacc
     6781 actaaatcaa tgggtttgat cgtatgcaaa gagccatctt ttttgccttt gccgcgcggt
     6841 gtacgcactg catcataaat aaaagcctct gttgacatcc tttatacctc tatctgtaat
     6901 tggactgtaa tttttaggat catttgcaaa acgcaatact gcacctaaac ttgatcggat
     6961 acgacgttta tatcaacatc atagccaaca ttttggatga tcgtacaaca aataattaaa
     7021 aaagaaaata ccaagcgatt gcttggctaa aattatttta ttttgctttg taacaagaac
     7081 tctcagtaaa aatataaaca gcaaatcgct tccttagcct gaacgttcat atttttcgta
     7141 cataaaccac tacccgtatc ctaataacac gttataatct caagcttttg taggtttaac
     7201 gtccaaaccg cttttaaaac tcaaaaacgt tttgatcctt ataaaatgac ataaacctgc
     7261 gttattaaca gacgactgca cttaaccatc atcgttaatt aggttcatcg tagaaattat
     7321 ttgtcttgga gtattcatgc gtagtagtgc cgatattcgc gaagcctttt taagcttttt
     7381 tgaaagcaaa ggtcacaccc gtgtagcatc cagctctctt attcctgcta atgaccccac
     7441 attattattt actaatgcgg gcatgaacca attcaaagac tgctttttgg gattagataa
     7501 acgtgcttat acccgtgccg ttagctcaca aaaatgcgta cgtgcaggcg gcaaacacaa
     7561 cgacttagaa aatgtcggtt acacggctcg tcaccatacc ttctttgaaa tgttaggtaa
     7621 ttttagtttt ggtgactatt tcaaacacga tgcgattgct tatgcgtggg agtttttaac
     7681 ctctcctgca tggttgaata ttccaagcga taaattatgg gtcactgttt acgccaccga
     7741 tgacgaagct ttccaattat ggcaagacgt ggctggtgta cctgctgaac gtattattcg
     7801 tattggtgac aacaaaggcg caccctatgc ctcagacaat ttctgggcaa tgggtgacac
     7861 aggcccttgt ggtccttgta ccgaaatttt ctatgaccac ggtgccgaca tttggggtgg
     7921 tttaccaggt agtgccgaag aagatggcga ccgttatatc gagatttgga ataacgtgtt
     7981 tatgcaattc aatcgcaccg ctgacggtgt attgcatcca cttcctgctc cttctgttga
     8041 tacaggcatg ggtttagaac gtattagtgc agtaatgcaa cacgttcata gcaactacga
     8101 aattgattta tttcaacatt tattacaagc cgctgccgat gcaattggtt gcagcaacga
     8161 acaacaagcg tctttaaaag tcgttgctga ccatgcgcgt tcttgtagct ttttagtggc
     8221 tgatggtgtt gtgccaagca acgaaggccg cggttatgta ttacgtcgta ttattcgccg
     8281 tgcttgtcgt catggcaaca aactcggtgc aacacaacct ttcttctata aaatggttgc
     8341 tgctttagct caagtcatgg gtgctgctta tcccgaattg gttgaacgtc aagcctttat
     8401 cgaaaaagtg ttgaagcaag aagaagaaca atttgcccgc acattggagc aaggtttaaa
     8461 aattcttgag caagatttag tcaacttaca aggcacagtc atcagtggtg atgtggtatt
     8521 taaattatat gacacctacg gcttcccaat ggatttaacg gcagatattg cccgcgaacg
     8581 tcatttaagc attgatgaag caggctttaa cgcttgtatg gatgcacaac gcgaacgctc
     8641 acaaaaagct ggcaagttta actttgacta caacagctta ctcaaagttg atgttgatac
     8701 tgtatttaca ggttatgaca gcacatgcag cgaaggcaaa gttgtggcca tgtttatcgg
     8761 tgatgaagcc gttaatcatg tcaacgaagg tcaaaaagcc gttgtagtct tagataaaac
     8821 ggctttttat gccgagtctg gcggtcaagt cggtgataca ggcgtattgt cagtgggtca
     8881 tacccgtttt gaagtacaaa acaccactaa agtgggtaac gcctttttgc atcatggcat
     8941 cgtcacacaa ggtcaactgt tagtaaatga taccgttgaa ggtcaagttg ataatacttt
     9001 acgcgccaac actgcacgca accactccgc gactcaccta ttacatgccg ccttacgcca
     9061 aacattaggc acacacgttc aacaaaaagg ttctttagtt tgcgctgatt atttacgttt
     9121 tgacttctca catacagaag gtgtcactgc cgaacaaatc agccaaattg aagccttagt
     9181 gaatggccaa atcttagcta atagcaccgt tgctactgaa gtcactgata ttgataccgc
     9241 caaagccaaa ggcgcaatgg ccttatttgg tgaaaaatat ggtgacacag tgcgtgtgtt
     9301 aagcatgggc acagacaact tctcggttga attatgtggt ggtacacacg ttagccgcac
     9361 aggtgatatt ggtttattaa aaatcactag cgaaggtggt gttgccgcag gtattcgtcg
     9421 tattgaagcc aaaacaggct caggcgcttt agcgtggtta gcggaaggcg aacaagccct
     9481 tgcccacatt ggccaattag tcaaaggttc tcgtcaagcc gttgccgata aagtgcaaca
     9541 actggctgaa cgtaatcgtg atttagaaaa agaaatcgaa cgcttaaaag caaaattagc
     9601 cagcagtgct agcaatgatt tattgtctca agtccaagac atcaacggcg ttaagttatt
     9661 agccgtcaaa ctcgaaggtg cagactctaa atccttgcgt acaacacttg accaattaaa
     9721 aaataaattg gcttctggtg tgattgtttt agcgacggtt gaaggcgaca aagttaccgt
     9781 gattgcaggg gtaacagccg acctcacctc gcgctttaaa gcaggtgatt tggtacaaca
     9841 tatttcaact cagttaggtg gcaaaggcgg cggtcgtcct gatatggcac aaggtggtgg
     9901 tacaaatgtt gccgccctgc ctcaagcgct taacagtgtc tctttgtggt tacagcaaaa
     9961 ataggaaatc tttactatgg cacttattgt tcaaaaatac ggtggcactt ctgttgggtc
    10021 acctgaacgt attcaagccg ttgccgaacg tgttgagcgt tggcataaac aaggccacga
    10081 tattgtggtt gtcgtttctg cgatgagtgg cgaaaccaat cgtttactcg ccttagctaa
    10141 agccattacg gatgagccag acccacgcga actcgatcaa atggtgtcaa caggcgaaca
    10201 agtcacgatt gccttattag caatggcctt acaaaaacgt ggtttaggtg ctaaatccat
    10261 gactggcgca caagttgcca ttcgtactga cagtgccttt accaaagcac gtatcgaatc
    10321 aattgatgat gctgctatgc gtgccgaact tgcaaaacgt aatgtgatta ttgttgcagg
    10381 tttccaaggt attgatgcac aaggcaacat taccacttta ggtcgtggtg gctcagacac
    10441 aacaggtgtt gcgctagcag ccgccttaaa agccgatgaa tgccaaattt ataccgacgt
    10501 tgatggtgtg tacaccactg acccacgtgt tgtacctcaa gcacgtcgtt tggaaaaaat
    10561 cacatttgaa gaaatgttag aaatggccag cctaggctct aaagttttac aaattcgctc
    10621 ggtcgaattt gcaggcaaat accaagtgcc attacgcgta ttgtctagct ttgctgaagg
    10681 cgaaggcaca ttaattacca cagattcgga ggacacaatg gaacaaccag ttatttcagg
    10741 tattgccttt aaccgtgacg aagccaaatt aaccattcgt ggcgtgccag acacccctgg
    10801 tattgcttat cgcattttag gcccaattgg tgatgccaat attgaaatcg acatgattgt
    10861 gcaaaacatc agtgccgaca acaccacaga ctttaccttt accgttcacc gcaacgaatt
    10921 ccgtaaagct ttacccattt tagaaaacat tgccaaagaa attggtgctc gtgaagtggt
    10981 tggcgacgac aagattgcta aagtatcttt agtgggtgtg ggtatgcgtt ctcacgctgg
    11041 tgtagctagc aaaatgtttg gcactttagc caaagaaaac atcaacatcc aaatgatttc
    11101 tacctctgaa atcaaaattt ctgtggttgt agaagagaag tatatggaat tagcagtacg
    11161 tgcgttacat actgcctttg gcttggatgc ttaag
//
LOCUS       DAOQMB010000033        11091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2287_length_11091_cov_15.589435, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000033 DAOQMB010000000
VERSION     DAOQMB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2287_length_11091_cov_15.589435"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1635
                     /gene="gpmI"
                     /locus_tag="PLY05_02425"
     CDS             100..1635
                     /gene="gpmI"
                     /locus_tag="PLY05_02425"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNC02188.1"
                     /translation="MRKPYVLIILDGFGHSETSDSNAIKAAHTPNWQQILQENANGLI
                     SGSGEDVGLPDGQMGNSEVGHMNLGAGRIVYQDFTRVTKSIRDGDFFTNPVITSAVDK
                     AVANQKAVHIWGLLSEGGVHSHEDHLHAMVKLAVERGAQHVYVHAFLDGRDTPPKSAM
                     PSLEKMDTALRATGKGRIASIIGRYYAMDRDNRWERVEQAYRLMTEGVAERTAPTAMA
                     ALTAAYEAGESDEFVKATAIIGEGEQAALINDGDAIVFMNFRADRAREISKAFVDTDF
                     TGFERRVRPQLAEFVMLTQYSASIKAPVAYKPEDLNNTLGEYLANLGKTQLRIAETEK
                     YAHVTFFFSGGREDLFAGEQRILVPSPKVATYDLQPEMSAYEVTDKLVAAIKSGEFDA
                     LICNYANGDMVGHTGVFDAAVKAVEALDVCLGRLYAAIKEVGGEMIITADHGNVEQMM
                     DNQTGQAHTAHTCELVPFAYVGRKATIKQGGVLSDVAPTLLSLMGLPIPAEMTGKVLI
                     DLQ"
     gene            1968..3389
                     /locus_tag="PLY05_02430"
     CDS             1968..3389
                     /locus_tag="PLY05_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC02189.1"
                     /translation="MKRLLNVALCSLVLHSYGWAAEATPTPDSNKKVENLPLTDLRTF
                     VDVFERIRQSYVDPVDDKTLFENAIKGMLSSLDPHSAYLDKDDYQDLKAQTTGEFGGI
                     GVEITMEDGYLKVISPIDDTPASKAGIKAGDYIVKLEGKAVKGMTMNEAVEVMRGEIG
                     KPLKLTIQRKGEAAQNLTLIRAKIETTSVKMRFIAADYPVIRITQFQTHTGRDLNKTL
                     NQLKEREKPIKGLILDLRNNPGGVLGGAISVADAFLDKGLIVYTKSRSEEPSEKFEAT
                     EGQNLPNVPLLVLVNGGSASASEIVAGALQDQKRAIIAGTTTFGKGSVQTVLQISEDK
                     AIKLTTARYYTPNGRSIQAEGIKPDVLIEPAKLELAAQGESYKEADLKGHLSNPNEQA
                     DATNNFKPTEEPKPVVDEEVKKGTDVGKKTKKSAKDDTKVEEKPVVKTDEIPTEQQLA
                     QDDYVLYAALNVLKGAAFWQAKP"
     gene            3371..4012
                     /locus_tag="PLY05_02435"
     CDS             3371..4012
                     /locus_tag="PLY05_02435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC02190.1"
                     /translation="MAGKTMIFAEDDLAPKATRVYQAIKDDREIIIDTLTRSFWDDPM
                     YNWLAKQDVYLGARFRRIFEIYWDRFAWPYEQIWTTEDRLGAALWSPPNCWQLGLAQQ
                     LAFLPDWLGVTGLSQLYSRWRAVDKIQAKHPHVPHWYLMAVGVAPEAQGKGYCSKLLR
                     PILDICDRTQTPAYLENSKEQNIEIYQHFGFEVTEEIIVDKEVKLWLMWREAR"
     gene            4109..5176
                     /locus_tag="PLY05_02440"
     CDS             4109..5176
                     /locus_tag="PLY05_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HNC02191.1"
                     /translation="MSSFEALTLHRLLLAVILSVVLFLGYSILSVFFIPMAWAGVLAY
                     VTWPAYTRLLTRLAYRHNLAAALMTLSLALLIIIPLLLAIIVLNIEVSNVYRVIEQKI
                     SSQNVVLPDFVKKWPFANELQKTLDNLMNNPDAFKEQLQAWFQKAFGAAAQVASGISK
                     NLAKLSMALFILFFFYRDGQKILQQIQQGLTVLIGDRVHGYIYAIGDTTRAVVYGIVL
                     TAVAQGFLAGIGYAFVGMESPIFLACITTLVAMIPFGTPFAWGSVAVWLLSQGYTVEA
                     LGLAAWGTFVISWVDNIIRPLVISSATEIPFLAVMFGVLGGLAAFGMIGLFIGPVILA
                     VLIAIWRQWLESEQNLAKTKS"
     gene            5324..7774
                     /locus_tag="PLY05_02445"
     CDS             5324..7774
                     /locus_tag="PLY05_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02192.1"
                     /translation="MKKIVWGLATVSVIGVGVHFATLPPPPPDVPPVPAKIPVPEVPA
                     LTPYDMPTGQVPVSTEPYRSTNRSVSIQDLVDRKLTTGIYTGTPYWKERAEWFDAHFY
                     DTLAARKVWNKGTSPDTHKRGGPVTWTYQPIPQYVPPPCTEVQPTGYDAEGCRYVLGL
                     FKDVEAKDPIKANKMREQARRGRDVWFKGTFGNQDENMLLQTRVVGKENMYYPWLDTR
                     TRAQRFSKWGLINDPDCVQGDASTNWYDKCQDPHSSGVLGYRKYYADPIKDASGKVVY
                     DPQTAPYSETEIKENKRYVIGHPCVQCHVGFDPTNPPKNPNEPKWENLSATIGNQHIR
                     QPLAFFQGAPSNHLANQAVMAGRLGTIDTSIVANDWQHNPGTQNNIMDFHNKRLFEHV
                     MKDPVTGIVKTAMTRHVLKGGEDSVGEHLALIRVYVNIGMCTEECWTPNFPYPGQFFG
                     RGTKQSPMRIMQCANKCDAWNYADSKMDDLAAFLITGGPTYLLAAKDDKDNTAGLNYI
                     DLKVVPKGREVFARECASCHSSKFAPENIRADKDALAKFYEGHIFGKEDYWRYEMSAE
                     KQKDAAFMAKYFVKDLKGQLRPKQFADKGVFGQDWLGNDERIPFNIVGTNMCRALHDN
                     HNQGHIWEEFSSETYKNSPSAGSVKQVFNRVIPLLGGVPFGERKIEGGPGYLRNISLL
                     SVWATAPFLHNNAIGEVTFLPDGSPDYTLSGRIKQFEMAFDELMMSDDPKVSPSRPQK
                     ITGLTEDFMSSGREDAQGLALIPFKKGEPAAHVLSSNPHRPLFMKCDDLVENKGHQFG
                     VDLTPQDKHALREFLKLM"
     gene            7915..9570
                     /locus_tag="PLY05_02450"
     CDS             7915..9570
                     /locus_tag="PLY05_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02193.1"
                     /translation="MAVISAIVLFNRPSTPPITELAKNTATKTNYLSQKIIPDTDLPP
                     QGTRSLFDHMIAQNNGLPYPFSKLIGLLKEQNPQGTEPITLLIPHGRSLLKGQADDKH
                     PRIVVAADFEQANAPAGLGLNTHGLLFLGFVENAHEIEVLSYNEAAGRFEFQLVQNYC
                     EGCVPRIVYARRAICTTCHQGGTPIFSQRPWNETNGQQSTAAAIAAARANNLAYENVA
                     LQQPLANSERFDQLTDVGNFYHVTQRLWLDGCGLDGNQCRRQMLKLALQYADNAGGFE
                     ANSAEAQVLKQLQAKNFPKEGIAVPESDLLNRDPIGEKQGFKGWLRSLVTRDIKFGEG
                     AKDNEDLSEFDKLPPLRKEVDPLTIRAPKQVLTAQHIDGVYGLARFFSDADLKTLAQA
                     NANQWANVIAKVQSLPDEVFAAKPFSRVVMMQALLGQSRDYCCLNTAEMSPPVVSGIP
                     PLTIKKLPQLQAFADHCFACHRGNPAQRLNFMAGDTEEQVLANIQAKKEIRDALDWER
                     YQGTEKASKLMPPRDSIQYQKLQQADEKTRQQMRDTVPSLFDF"
     gene            9582..10349
                     /locus_tag="PLY05_02455"
     CDS             9582..10349
                     /locus_tag="PLY05_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02194.1"
                     /translation="MRLTFMLLTSSLLITSVNSYAMPAFARYYKQQYGYMPSCHACHS
                     EGGGTPLNNYGKAFKENGKSLAALSKIANLDSDGDGSANGVEAQAKANPADKNSIPAK
                     VGTWLDLASLIPKEVQALFPAIRSWLPKDAVLTAADIATAKTMGATLSAADENTIYIP
                     VENQRPAGTALIFPAQYQGKTFFLLMATDKQLKITQVKVMNAKDVPAAKSSAVYGKLV
                     GVAAQSVPAPSSKDLDAAITQAVKNAGVLLYVRLKGA"
     gene            10352..11086
                     /locus_tag="PLY05_02460"
     CDS             10352..11086
                     /locus_tag="PLY05_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02195.1"
                     /translation="MWRLIAVALCLLSSWVWGAEPDINFFSDQPIPEAALVHTPEPKP
                     DWLLYGAPIALLIFFFSFCLIVKLLIPFKETDMRFDLHDLPVAAQRGIGMAVILFGIA
                     FCFGGLEAHYQMGLHGSAEAYFGQMGLGKLIAFTHAHLFGFTTSYFIIGIPFSLHFNR
                     LKIYQWIFPLGLAASLTDVISWWGIKFVSPYFEYVTWWCGFVFSACYLWMLVALVRVL
                     FFPRVKWFPDFINEDRQKKWDEEHRK"
BASE COUNT         3237 a         2048 c         2496 g         3310 t
ORIGIN      
        1 tttgtctttt tttatcaaga cttgtcgtaa aaagcttaaa aaatagcgta acactgctaa
       61 aatacgtgcc ttatttttgg ctgttatgag agtggtgtta tgcgtaagcc ttatgtgtta
      121 attattttgg atggttttgg tcacagcgaa acctctgatt ccaatgcaat taaagcggcg
      181 catacgccta actggcaaca aattttacaa gaaaatgcca atggtttaat ttcgggttct
      241 ggcgaagacg tcggtttacc tgatggtcaa atgggtaact ccgaagtcgg tcacatgaat
      301 ttaggggcag gacgtattgt ttatcaagac tttacccgtg ttaccaagtc gattcgtgat
      361 ggtgattttt tcactaatcc tgtgattaca tctgctgttg ataaagccgt tgccaatcaa
      421 aaagcggtgc atatttgggg cttattatcc gaaggtggtg tgcattccca cgaagaccat
      481 ttacacgcga tggttaagtt ggcggttgag cgtggtgcac aacacgttta tgttcatgcc
      541 tttttagatg gtcgtgacac cccacccaaa agcgcaatgc cctccttaga aaaaatggat
      601 actgccttac gtgcgacagg caaaggtcgt attgcctcga ttattggtcg ttattatgcc
      661 atggacagag ataatcgttg ggagcgtgta gaacaggctt atcgtttaat gactgaaggt
      721 gttgctgagc gtactgcacc tactgcaatg gcagcattaa cggcggctta tgaagcaggt
      781 gagagtgatg agtttgttaa agccaccgcg attattggcg aaggtgagca agccgcctta
      841 attaatgatg gtgatgcgat tgtgtttatg aatttccgtg ctgaccgtgc gcgtgaaatt
      901 tccaaagcct ttgttgatac agactttaca ggttttgaac gtcgtgtccg tccgcagtta
      961 gccgaatttg tcatgttgac ccaatattct gccagcatca aagcacctgt ggcttataaa
     1021 cctgaagatt taaacaatac cttaggtgag tatttggcta acttgggcaa aacgcaatta
     1081 cgtattgctg aaaccgaaaa atatgcccac gtgaccttct tctttagtgg tggccgtgaa
     1141 gacttatttg caggtgaaca acgtatttta gtgccatctc ccaaagtggc gacttatgat
     1201 ttgcaacccg agatgagtgc ctatgaagtc actgacaaat tagtggcagc gattaagtcg
     1261 ggtgaatttg atgccttaat ttgtaattat gctaatggcg atatggtcgg gcatacaggc
     1321 gtgtttgatg cggcagtgaa agcggttgaa gccttagatg tgtgtttagg tcgtttgtat
     1381 gcggctatta aagaagtcgg tggcgaaatg attattaccg ctgaccacgg taacgttgag
     1441 caaatgatgg ataaccaaac aggtcaagcc catactgcac atacctgtga attagtgcca
     1501 tttgcctatg tgggtcgtaa ggcgacgatt aaacaaggtg gcgtgttgtc ggatgttgcc
     1561 cccacgttat taagcttaat ggggctacca attcctgccg aaatgacagg taaggtgttg
     1621 attgatttgc aataaaggct aaagaaaacg tagcgtgggt agaggcgcga aacccacgat
     1681 aatcaaatgt taattgtggg tttctacgtc aacccacaat taattagccc atcgtttaat
     1741 cccctaaata aaatgccttg tttgggaaaa tcgggtacac aacgaatata ttgctcaagt
     1801 ttggtgttgg gcataacaaa tatcctttaa tcaacaatca atatcgctta accttgtcag
     1861 gtcataataa caataataca actacttgta tgattgatgt gcatgaccat tagaattaat
     1921 gctgttaatc taaaaatatg gtttgctaat aggattttga gaaataaatg aaacgcttgt
     1981 taaatgtcgc cttatgttct ttagtattac actcctatgg atgggcagca gaggcaacac
     2041 ccacaccaga cagtaataaa aaagtagaaa atttacccct caccgattta cgcacctttg
     2101 ttgatgtttt tgagcgtatt cgtcaaagtt atgttgaccc tgttgatgat aaaaccttat
     2161 ttgaaaatgc cattaaaggt atgttgtcat cacttgaccc tcattctgcc tatttagata
     2221 aagatgatta tcaagattta aaagcgcaaa ccacaggcga gtttggcggt attggtgtcg
     2281 aaattacgat ggaagatggt tatttaaaag tcattagtcc gatagatgat acacccgctt
     2341 ctaaggctgg catcaaagca ggtgattata tcgtcaaatt ggaagggaaa gcggttaaag
     2401 gcatgaccat gaatgaagct gtggaagtga tgcgtggaga aattggtaaa ccactcaaat
     2461 taaccattca acgcaaaggc gaagccgcac aaaatttaac cttgattcgt gccaaaattg
     2521 aaactaccag cgttaaaatg cgttttattg ccgcagatta tcctgtcatt agaattactc
     2581 aatttcaaac tcatacaggc cgtgatttaa ataaaacctt aaatcaactc aaagaacgcg
     2641 aaaaaccgat taaaggctta attttagatt tacgcaacaa tccgggtggc gtgttgggtg
     2701 gcgcaattag tgttgcggat gcctttttag ataaaggttt gattgtttat accaaatcgc
     2761 gtagcgaaga acctagtgaa aaattcgaag cgactgaagg acaaaattta cccaatgtgc
     2821 cgttgttagt attagtcaat ggtggttctg catcggcttc cgaaattgtc gcaggggcat
     2881 tacaagacca aaaacgcgcc attattgcgg gaacaaccac ctttggcaaa ggctcggtgc
     2941 aaaccgtttt gcaaatcagt gaagacaaag ccattaagtt aaccactgcg cgttattaca
     3001 cgcccaatgg ccgttcgatt caagccgaag gtattaaacc tgacgtgtta attgagccag
     3061 ccaaactaga gcttgctgct cagggagaaa gttataaaga agcggattta aaaggtcatt
     3121 tatcaaatcc caatgaacaa gcagatgcaa ccaataactt taagcctacc gaagagccaa
     3181 aacctgtggt tgacgaagag gtgaaaaaag gcacagatgt cggtaaaaag accaaaaaat
     3241 cggcaaaaga tgatactaaa gttgaagaaa aaccagttgt taaaacggat gaaataccta
     3301 cagagcagca attagctcaa gatgattatg tgttgtatgc tgctttaaat gtgttaaagg
     3361 gcgcagcttt ttggcaggca aaaccatgat ctttgcagaa gacgatttag ctcctaaagc
     3421 gacgagagtg tatcaggcaa ttaaagatga tcgagaaatt atcattgata cgttaacacg
     3481 ctcgttttgg gatgacccga tgtataactg gttggccaaa caagatgtct atttaggggc
     3541 gaggtttcgg cgaatttttg aaatttattg ggatcgtttt gcatggccgt atgagcaaat
     3601 ttggacaacc gaagatcgtt tgggagcagc tttgtggtca ccacccaatt gttggcaatt
     3661 gggtttagct cagcaattag cctttctgcc cgattggcta ggcgtcacag gtctaagtca
     3721 gttgtattcc cgttggcgag cggtggataa aattcaagcc aaacatccgc atgtgcctca
     3781 ttggtattta atggcggtcg gggttgcacc cgaagcgcag ggcaaaggtt attgctccaa
     3841 gttgttaagg ccaatattag atatttgtga tcgtacccaa acgccagcct atttagaaaa
     3901 ctctaaagaa caaaatatcg aaatttatca acactttggt tttgaggtga ctgaggagat
     3961 tattgttgat aaggaagtta agttgtggtt aatgtggagg gaagcgcgtt aaatagcggt
     4021 ggatatatga gttattttca aaaaccatct tggtagttaa tctgattccc agttatagtc
     4081 ggttgacgca ttttaaggaa atgatcgaat gtcttcattt gaagctttaa cccttcaccg
     4141 attattacta gccgttattt tgagtgttgt cctatttttg ggctacagta ttttatcggt
     4201 tttttttatt ccaatggcat gggcaggcgt tttagcctat gtaacatggc cagcctatac
     4261 acgtttgtta acacgcttag cctatcggca taatttagca gccgcattaa tgaccttgag
     4321 cttagcctta ttaatcatca ttcctttatt attagcaatt attgtgttaa atatcgaagt
     4381 aagtaatgtt tatcgagtta ttgagcaaaa aatcagtagt caaaatgtag tactaccaga
     4441 ttttgtaaaa aaatggccat ttgccaatga gttacaaaaa actttagaca atttaatgaa
     4501 taatcctgat gcttttaaag aacagttgca agcatggttt caaaaagcat ttggtgcagc
     4561 ggcacaagtg gcaagtggta ttagcaaaaa cttagccaaa ttatcaatgg cattgtttat
     4621 cttatttttc ttttatcgtg atggccaaaa aatattgcaa caaatacaac aaggtttaac
     4681 cgttttaatt ggtgatagag ttcacggtta tatttatgca attggtgata caacccgagc
     4741 tgttgtttat ggtattgtgt taacggctgt tgcccaagga tttttagcag gaataggcta
     4801 tgcttttgta ggcatggaaa gtccaatatt tttggcctgt attaccacct tggtggcgat
     4861 gattcccttt ggaactccct ttgcgtgggg gagtgtggcg gtttggttat tgagccaagg
     4921 ttataccgtt gaagcattgg gtttggcagc gtggggaact tttgtgatta gctgggttga
     4981 taacattatt cgcccccttg tcatttctag cgcgaccgaa ataccttttt tagcagtgat
     5041 gtttggagta ttgggaggct tagctgcttt tggcatgatt ggtttattta ttggaccagt
     5101 tattttagcg gtattaatcg ctatttggcg ccagtggcta gaaagcgaac agaatttagc
     5161 aaaaacaaag agttaagttg ttaaatctaa aaatggagtt tttactctag aataattact
     5221 ctaaataatt gaattaaaac aaaaaattag agcttttgca ccagttgttt atttgtatta
     5281 atctcgttaa actggcgcaa aattgctcaa tctaggagcc aacatgaaaa aaatagtttg
     5341 gggtttagcc acagtatcag tgattggtgt gggggtgcat tttgctactt tgccgccacc
     5401 tccgccagat gttccacctg taccagccaa aattcctgtg ccagaagtgc cagctttaac
     5461 accttatgat atgccaactg gtcaagtacc tgtatcaaca gaaccatatc gttcaacaaa
     5521 tcgcagtgta tccattcaag atttagttga tcgtaaatta acaacaggta tttatacagg
     5581 tacaccttat tggaaagaac gtgccgaatg gtttgatgcg catttttatg acaccttagc
     5641 tgcgcgtaaa gtgtggaata aaggtacatc acctgatact cacaaacgtg gtgggccagt
     5701 cacatggaca tatcagccta ttccacagta tgtaccgccg ccctgtaccg aagtgcagcc
     5761 aacgggttat gatgccgaag gatgccgtta cgttttggga ttatttaagg atgtagaggc
     5821 taaagacccc attaaggcca ataaaatgcg tgaacaggcg cgtcgtggtc gtgatgtctg
     5881 gtttaagggc acgtttggca accaagatga aaacatgttg ctgcaaacgc gtgtcgtcgg
     5941 taaagaaaat atgtattacc cttggttaga cacacgtact cgcgcacaac gttttagcaa
     6001 atggggttta attaacgatc ctgattgtgt gcaaggtgat gcttctacta attggtacga
     6061 taaatgccaa gacccacatt catcaggtgt gttaggttat cgtaaatact atgccgaccc
     6121 aattaaagat gcttcgggta aagttgttta cgatccacaa acagctcctt attccgaaac
     6181 agaaattaaa gaaaacaaac gttatgtgat tggtcatccc tgtgtgcaat gtcacgtggg
     6241 ttttgatcct actaatccac ccaaaaaccc gaatgaacct aaatgggaaa atttaagtgc
     6301 cacgattggt aaccaacaca ttcgtcagcc cttagctttc ttccaaggtg cgccaagtaa
     6361 tcatttagcc aatcaagctg tgatggcagg acgcttaggt acaattgata cctcaattgt
     6421 tgctaacgat tggcagcata atcctggcac acaaaacaat attatggact ttcataacaa
     6481 acgtttattt gaacacgtga tgaaagaccc agtaactggt attgtgaaaa cggccatgac
     6541 acgccatgtg ttaaaaggtg gtgaagactc tgtgggcgaa catttggcct taattcgggt
     6601 atatgtcaat attggtatgt gtaccgaaga atgttggaca ccgaactttc cttatcctgg
     6661 tcaatttttt ggtcgtggaa ctaagcaaag cccaatgcgt atcatgcaat gtgccaacaa
     6721 atgtgatgcg tggaattatg ctgatagtaa gatggatgat ttagccgcct tcttaatcac
     6781 aggtgggcca acctatttat tagcagctaa agatgataaa gataataccg ctggtttaaa
     6841 ttatattgat cttaaagtag tccctaaagg tcgtgaagtt tttgctcgtg agtgtgcttc
     6901 ttgtcatagc tctaagtttg cacctgaaaa tattcgtgcc gataaagatg ctttagcgaa
     6961 gttttatgaa ggccatattt ttggtaaaga agattattgg cgttatgaaa tgagtgctga
     7021 aaagcaaaaa gatgcagcat ttatggctaa atactttgtg aaggatttaa agggtcaatt
     7081 acgtcccaaa caatttgctg ataaaggtgt ttttggtcaa gattggttag gtaatgacga
     7141 acgtattcct tttaatattg tcggtactaa tatgtgtcgt gccttgcatg ataaccataa
     7201 tcaaggtcat atttgggaag agttttcttc tgaaacttac aaaaatagtc cctctgctgg
     7261 cagtgtcaaa caagtcttta accgcgtgat tcctttgctt ggtggcgtgc catttggcga
     7321 gcgtaaaatt gaaggcgggc cgggttattt gcgtaatatc tcgttattat cggtttgggc
     7381 aaccgctcct ttcttgcata ataatgcgat tggtgaagtg actttcttgc ctgatggttc
     7441 accagattac actttaagtg gtcgcattaa acaatttgaa atggcgtttg atgagttgat
     7501 gatgtctgat gaccccaaag tctcacccag tcgtccacaa aaaatcacag gactgaccga
     7561 agactttatg agtagtggtc gcgaagatgc ccaaggttta gcattgattc ccttcaaaaa
     7621 aggtgagcct gctgcacatg tgttatcttc taatccacat cgtcctttgt ttatgaagtg
     7681 tgatgatttg gttgaaaata aaggtcatca atttggtgtg gatttaacac ctcaagataa
     7741 acacgctttg cgtgaattct tgaaactgat gtaaatcacc ccctctccca cagggagagg
     7801 gtcggggtga gggaataaat aacctcaccc tgactctaaa ctaggggatt gtacgtttta
     7861 aaatttagaa gtcatatgaa aaaaataata cttgggattt cgctaattgt ggtgatggct
     7921 gttatatcag caatcgtttt atttaaccgt ccaagtacac caccaattac tgaattggct
     7981 aaaaataccg ctaccaaaac aaattattta tcgcaaaaaa ttattcctga caccgatttg
     8041 ccgccacaag gtactcgctc gttatttgat cacatgattg cccaaaataa tggtttacct
     8101 tatcccttta gtaaattaat tggtttatta aaagaacaaa atccgcaagg tacagagcca
     8161 atcaccttat taattccgca tggtcgctcc ttattaaaag gccaagccga cgataaacat
     8221 ccgcgtattg tcgttgcggc tgattttgaa caagccaatg cacccgcagg cttaggctta
     8281 aatacgcatg gtttattgtt tttaggtttt gtggagaatg ctcacgaaat tgaagtatta
     8341 agttacaacg aagcggcagg gcgttttgag tttcaattag ttcaaaatta ttgcgaaggc
     8401 tgtgtcccca gaattgtcta tgcgcgccga gccatttgta ccacctgtca ccaaggtggc
     8461 acgccgattt tctctcagcg tccgtggaat gaaaccaatg gccagcaaag cacggcagca
     8521 gcgattgcgg cggcacgcgc taataatctc gcgtatgaaa atgtggcatt acaacagcca
     8581 ctcgcaaatt ctgagcgttt tgaccagttg actgatgttg gtaattttta tcatgtcact
     8641 caacgtttat ggttagatgg ttgtggttta gacggcaatc aatgtcgtcg gcaaatgtta
     8701 aaactcgctt tacaatatgc cgataatgca ggcggctttg aagctaatag tgctgaagca
     8761 caggttttaa aacaattaca agcaaaaaac tttcccaaag aaggtattgc ggtgcctgag
     8821 tcagatttat taaaccgtga tcccattggg gaaaaacaag gttttaaagg ttggttacgt
     8881 tctttggtga cgcgtgatat taagtttggt gaaggtgcaa aagacaacga agatttaagc
     8941 gagtttgata aattgccgcc tttacgcaaa gaagttgacc cattgaccat ccgcgcccct
     9001 aaacaagtct taaccgcaca acacattgat ggtgtgtatg gtttagcacg attttttagc
     9061 gatgccgatt taaaaacttt agcccaagcc aatgctaatc aatgggcaaa tgtcattgca
     9121 aaagtgcaaa gtttgcctga tgaagttttt gccgctaagc ccttttcacg ggtggtcatg
     9181 atgcaagctt tgttgggaca atcacgtgat tattgttgtt tgaatacggc agaaatgtct
     9241 ccgcctgtgg tgtcaggtat tccaccttta accatcaaaa aattgccaca attacaggca
     9301 tttgctgatc attgttttgc ttgtcatcgt ggtaatcctg cacagcgttt aaactttatg
     9361 gcaggggaca ccgaagagca agttttagcc aatattcagg ctaaaaaaga aattagagat
     9421 gcgttggatt gggagcgtta tcaaggcaca gagaaagcca gtaaactgat gccaccacga
     9481 gattctatac agtatcaaaa actacaacaa gcagacgaaa aaactcgcca acaaatgcgc
     9541 gatacggtgc caagtttatt tgatttttga gggaaaagat catgcgttta acattcatgc
     9601 tattaaccag tagtttatta ataactagcg tcaatagtta tgccatgcca gcctttgctc
     9661 gttattacaa acaacaatat ggttatatgc cctcatgtca tgcttgtcat agtgaagggg
     9721 gcggtacacc attaaataac tatggcaaag ccttcaaaga aaatggtaaa tctttagcag
     9781 cgttaagcaa aattgctaac ttagacagtg atggtgatgg ctctgcaaat ggtgttgagg
     9841 cgcaagccaa agcaaatcct gccgacaaaa attcaatccc tgctaaagta ggaacatggt
     9901 tagatttagc cagtctaatt cctaaagaag tccaagcctt atttccagct attcgttcat
     9961 ggttacccaa agatgcagtt ttaaccgctg ccgatattgc cactgccaaa acaatggggg
    10021 caacgttgag cgcggctgat gaaaatacta tttatattcc tgttgaaaat caacgccctg
    10081 ctggtacagc gttaattttt cctgcccaat atcaaggcaa aactttcttt ttgttaatgg
    10141 caaccgataa acaactcaaa attacccaag ttaaagtgat gaatgccaaa gatgtgcctg
    10201 cggcgaagag tagtgcagta tatggaaaat tggtgggtgt agcagcacaa agcgttcctg
    10261 caccaagctc aaaagacctt gatgcggcga ttactcaagc ggtaaaaaat gcgggtgtct
    10321 tattgtatgt gcgcttaaaa ggagcgtaat catgtggcga ttaatagcgg tagcattgtg
    10381 tttgttgagt agctgggttt ggggtgcaga gcctgatatt aattttttta gtgatcagcc
    10441 cattcctgaa gcagcactgg tacatacgcc agaacccaaa cctgattggt tattatatgg
    10501 tgcgcctatt gctttattaa tcttcttttt tagtttttgt ttgatagtta agttattgat
    10561 tccctttaaa gaaaccgata tgcgttttga cttgcatgac ttacctgttg ctgcacaacg
    10621 tggtattggt atggcggtga ttttatttgg tatcgctttt tgttttggcg gtttagaagc
    10681 gcattatcaa atgggtttac atgggagtgc cgaagcttat tttggccaaa tggggttggg
    10741 taagttaatt gcgtttaccc atgcacattt atttggtttt accaccagtt attttattat
    10801 tggtattcct ttttctttac atttcaatcg cttaaaaatt tatcaatgga tatttccttt
    10861 gggattagcc gcctctttga cagatgttat ctcgtggtgg ggcattaagt ttgtgtcacc
    10921 atattttgaa tatgtaacgt ggtggtgtgg ttttgtgttt agtgcttgtt atttgtggat
    10981 gttagttgcc ttagtgagag tgttgttttt tccgcgtgtt aaatggtttc ctgattttat
    11041 taatgaagac agacaaaaaa agtgggatga agaacatcgc aaataaaaaa g
//
LOCUS       DAOQMB010000034        10695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2384_length_10695_cov_14.446147, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000034 DAOQMB010000000
VERSION     DAOQMB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2384_length_10695_cov_14.446147"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..858
                     /gene="ruvA"
                     /locus_tag="PLY05_02465"
     CDS             262..858
                     /gene="ruvA"
                     /locus_tag="PLY05_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017398135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNC02196.1"
                     /translation="MIGRLKGIVIEKNAPQIIIDVNGVGYEVETPLPTFCRLRLGENE
                     TLWTHLVVREDAHLLYGFSEKEERALFRLLLKVSGVGPKLALALLSGMEPLAFLRCIE
                     AEDISTLTKIPGVGKKTAERLLIELRDRIKELTPQSATPTERLTLTSPLSPVAEAEQA
                     LMALGYKPQEAQKAVALVKNQHDDTAGLIRAALKAMVK"
     gene            complement(1075..2025)
                     /gene="sppA"
                     /locus_tag="PLY05_02470"
     CDS             complement(1075..2025)
                     /gene="sppA"
                     /locus_tag="PLY05_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004682695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNC02197.1"
                     /translation="MSENNGREWQLLEKVALASIDEQRKARRWGIVFKLLTFTYLVVI
                     LIMVTSGDKHGGKATESSADHVAIVELKGEIADGADANADDLIGSLTDAFEAKNSKAV
                     VLRVNSPGGSPVQSAYVYDAIRQLRDKYPNKKFYAVITDIGASGAYYIASAADQIYVS
                     PASLVGSIGVIMPNFGVQGLTQKLGIEDRTMTAGEHKAIMNPMAPITAEEKAHVQKML
                     NTIHQQFITAVKQGRGQRLKEDPQIFSGLFWTGQQAVELGLADGFGSVKTINKLAGTD
                     EQVDYTQGKNPMDNLIRKLGASMGETIAAKLDLGNRLEVR"
     gene            complement(2038..2685)
                     /locus_tag="PLY05_02475"
     CDS             complement(2038..2685)
                     /locus_tag="PLY05_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC02198.1"
                     /translation="MPAYKLAIFDWDGTLMDSVAHIVDSMQQAAQVLGEPVPSVSEVR
                     HIIGLGLPEAIALLFPHASLSTREEIRQQYAKHFLAHSASKSELFTGAQSLLDQLQQQ
                     GYLLAIATGKSRLGLDRVLSQTGIGHYFVATRCADETASKPDPLMLRQLLAYTGSSAE
                     QSVMIGDTSYDMEMAQALDMPRIAVSYGVHSKDMLQSYQPLAIVDSIYQLQDYLI"
     gene            complement(2672..3634)
                     /gene="rluC"
                     /locus_tag="PLY05_02480"
     CDS             complement(2672..3634)
                     /gene="rluC"
                     /locus_tag="PLY05_02480"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HNC02199.1"
                     /translation="MTDSNSSPAVRLITITENQDGQRLDNFLITALKGAPRTLVYRIV
                     RTGEVRVNKGRVKPEYRLQIGDVVRVPPVRLAEKEDVVVPSQQLSNSLANRIIYRQDG
                     IIVLNKPSGLPVHGGSGVAYGAIEALRAIYPQTEFLELVHRLDRDTSGLLMISDKRSV
                     LRVLHEQLRDGKMQKVYLALLAGKLKGANHKVTAPLDKNNLASGERVVRVSREGKEAE
                     THFKVIERFEDCTLVEARPLTGRTHQIRVHAQFLGHPILGDEKYCSNELNKTAKDKWG
                     LNRLFLHAQDVSLQLPNQEKPLQLNCPLAPELVQVLEKLRNARV"
     gene            4243..>5069
                     /locus_tag="PLY05_02485"
     CDS             4243..>5069
                     /locus_tag="PLY05_02485"
                     /inference="COORDINATES: protein motif:HMM:NF021653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HNC02200.1"
                     /translation="MKRMLINASHAEETRVALVDGQRLYDFDLEHHSRVQKKANIYKG
                     RVTRVEPSLEAAFVDFGADRHGFLPLKEIAREYFHRQPRDVGRVTIRDAIQEGQEIIL
                     QVEKEERGNKGAALSTFISLAGRYLVLMPNNPRAGGISRRIEGEEREELKEALNSITI
                     PNDMGVIVRTAGLGRSPEELQWDLDYLLTLWKSISQAAEQSPAPFLIYQESNVIIRAI
                     RDYLRDDIDEVLIDSEAAYNQAIDFVRQVMPHFQNKIKLYRDTVPLFNRYQIESQIET
                     "
     assembly_gap    5070..5169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5170..7308
                     /locus_tag="PLY05_02490"
     CDS             <5170..7308
                     /locus_tag="PLY05_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02201.1"
                     /translation="MDRARVQIGRISRFGLLELSRQRLRPSLEETTGLVCPRCNGTGV
                     IRDVRSLALSIMRVIEEEVLKDRSAEIQAQVPVTVATFLLNEKRAAISALEARSGVRI
                     IILPNPHLETPHYDVSRLRDDHSDDDYEAPASFQQVENFRPVLEDNGLYNSEEKTQTR
                     RQEAAVKMVQPETQAPVPRATTPTQVITRQEKEQSKTGFFAWHANLFSGVSELANEID
                     SKLAVSRPAPSYNNSENFVTNDNARSQRPQRENRTNRAEPEFRMESRDVAPTRQTRVE
                     IEATPAPVREPRPQRERPQRVEQDFRSEHHVNVSPRQVRHTEVVEPITPPVREPRPQR
                     ERPQRENRTHRADALEVNSAALAIDAPVAPIAEQEEQRRPRRERPSGHGFNRRQRPPR
                     ERDAQVLAETTFDATVPDSNVTPVAFEPITAELVTASDDMPVVVEPVSSQEQEIVIAS
                     EATVEMALPEATVETSNEAPEPVAENTAQTTVTPVDSEVITPEATVKAEEVVAPVIET
                     TVIEAAPTRAANDPRERRRREREAAERAAREEAERLVAEQTAKEAAERLAIELAALAA
                     EQAQKTQATPAVMPEATWRTVGEFIAAFTPEVKAITGAEVVSAFMVAQKKQQEIAAAI
                     AQTEATPPVAEETATITEVLVDTTVAEPVVEVIANSEDASAAVSLPVSNTWYTVGEFI
                     AAFIPDAKPITGTEIVSAFMLAQKKQQELG"
     gene            complement(7410..7502)
                     /locus_tag="PLY05_02495"
     tRNA            complement(7410..7502)
                     /locus_tag="PLY05_02495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7466..7468),aa:Ser,seq:cga)
     gene            complement(7557..7790)
                     /locus_tag="PLY05_02500"
     CDS             complement(7557..7790)
                     /locus_tag="PLY05_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02202.1"
                     /translation="MKNMAKVVVVLGLVSSLSGCFLTKVVTMPMRVVGATTSMVGAVV
                     SVVPVAGNKVDAALEKADTTIDTAADTVDDVPL"
     gene            complement(7804..8517)
                     /locus_tag="PLY05_02505"
     CDS             complement(7804..8517)
                     /locus_tag="PLY05_02505"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNC02203.1"
                     /translation="MEKRELLYTGKAKSVYTTDNPDYLVLVFRDDTSAFDGKKVEQLA
                     RKGRVNNTFNAFIMQKLAEAGIETHFEKLITPNEVLVKRLTMIPVECVVRNYAAGSLC
                     RRLGVTEGLELNPPTFELFLKNDALGDPMINESHVRSFGWATEAQLARMKELSFAVND
                     VLKALFAQGDMLLVDFKLEFGVFHDRIVLGDEFSPDGCRLWDKETRKKLDKDRFRQGL
                     GEVVEAYEEVGRRLGINFE"
     gene            complement(8581..9189)
                     /gene="bamC"
                     /locus_tag="PLY05_02510"
     CDS             complement(8581..9189)
                     /gene="bamC"
                     /locus_tag="PLY05_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HNC02204.1"
                     /translation="MRLWLSGFGLVLLSGCSLFPDRSLDYLQAQSIQPLPTDNQTRSI
                     KPLYQIPVVTTPAEQAAQIVQGKGRKQEFVAPSPKPLVVTESEQTVSKTQIIVEQKPS
                     LAFDGNQHPVLTITGEQLKLWEQLGQAINAAKVKITDRNQSSGLYFIELSQTNNKKEA
                     YLLKLTSTAAKTVVSLQKDEDTLADSVLSQQILTELIKHWPS"
     gene            complement(9191..10063)
                     /gene="dapA"
                     /locus_tag="PLY05_02515"
     CDS             complement(9191..10063)
                     /gene="dapA"
                     /locus_tag="PLY05_02515"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019205078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNC02205.1"
                     /translation="MITGSLVALVTPMHNNGDVDWSALKSLVEWHVAQGTHGIVAVGT
                     TGESATLDVEEHCAVIKCVVETVNKRIPVIAGTGANSTREAIELTRSAKQAGADACLL
                     VTPYYNKPTQEGLYQHYKAVAEAVDIPQILYNVPGRTGVDMSNATVARLAGTPNIIGI
                     KDATGNLERGQELLNLVGDKMAVYSGDDATAYQLILMGAKGNISVTANVAPKAMSDVC
                     EAALAGNAQAAADLNAPLVALHKDLFVESNPIPVKWALFAMGRIELGIRLPLTVLADQ
                     CQPVVRAALQQAGI"
     gene            complement(10237..10312)
                     /locus_tag="PLY05_02520"
     tRNA            complement(10237..10312)
                     /locus_tag="PLY05_02520"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10277..10279),aa:Gly,seq:gcc)
     gene            complement(10459..>10695)
                     /locus_tag="PLY05_02525"
     CDS             complement(10459..>10695)
                     /locus_tag="PLY05_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNC02206.1"
                     /translation="ISALREWMAELGKRANVAVSQIGKWKTTAQMASITILLIKDPTL
                     NYLGFVLLYIAVVLTIWSMLIYLKAAWPDLSEAD"
BASE COUNT         3194 a         2373 c         2014 g         3014 t
ORIGIN      
        1 gtgtgtgagc tgcgttgctg ataaaaattg actcaggcaa ggcacaaaac gcagcgaata
       61 gttattctat tagcaagttt tgtaacgtag aatgagtcaa tttttaacgc aacccacagg
      121 gaaagagcta ttttttgagg ctactttgtt gctcatcgct tatttggaat aaccaaacta
      181 cgcgattcac gcctcgtatc ctctaaaaaa tagcttcttt cgcaaccaca atcaaaacgt
      241 taacagaccc taggatttat tatgattggc cgtttaaaag gcattgtgat tgaaaaaaat
      301 gcaccacaaa ttattattga tgtgaatggt gtaggttatg aagtagaaac gcccctgcca
      361 acgttttgcc gtttgcgttt gggtgaaaat gaaactttat ggacacattt agtggtgcgt
      421 gaagatgccc atttgttata tggttttagt gaaaaagaag aacgtgcttt atttagattg
      481 ttgttaaaag tcagtggtgt tggccctaaa ttagcgttag cgttattgtc gggcatggaa
      541 cctttggctt ttttacgttg tattgaagcc gaagatattt ctacgttaac caaaattccc
      601 ggtgtgggta aaaagacggc agaacgcttg ttaattgaac tacgcgaccg aattaaagag
      661 ttaacaccgc aaagtgccac acccacagag cgtttaactt taaccagtcc tttgtcacct
      721 gtggcagaag ccgaacaagc gttaatggca ttgggctata aaccgcaaga ggcacaaaaa
      781 gcagtggctt tagtcaaaaa tcagcatgat gatacagcag gtttaattag agctgcttta
      841 aaagcaatgg ttaaataacg gccagtttta gcccatatcc atgtttagta gcccgtgtga
      901 agtgcaacgt aacacgggat ttatgccaac actaaagcaa taagtatttg ttttaatata
      961 tttaatccct tatttcgcga tgttccatac gggctatgct tgctttttta ttgaatccgc
     1021 cgtcaataaa aaagcccctt aaaaattaag aggctttttt ggtttagcta aaaactaacg
     1081 gacttctaaa cgattaccca aatctaactt agcggcaatc gtttcaccca tgcttgcacc
     1141 caatttacga attaaattat ccattggatt tttaccttgg gtgtaatcaa cttgctcatc
     1201 tgtgcctgct aatttattaa tagtttttac cgaaccaaaa ccatccgcca aacctagctc
     1261 aaccgcttgt tgacctgtcc aaaataaacc tgaaaaaatt tgcgggtctt cttttaaacg
     1321 ttgaccacga ccttgtttga ctgcggtaat aaattgctga tgaatggtgt tgagcatttt
     1381 ttgcacatgg gctttttctt cggcagtaat tggtgccata gggttcataa ttgctttatg
     1441 ctcacctgcg gtcatagtac ggtcttcaat acctaacttc tgagttaaac cttgcacacc
     1501 aaaattaggc ataatcacac caatcgaccc tactaaactg gcagggctaa cataaatttg
     1561 gtcagcagca gaggcaatat aataagcacc tgatgcgcca atatcggtaa tcactgcata
     1621 aaatttttta ttaggatatt tatcacgtaa ttggcgaatc gcatcataca cataggctga
     1681 ttgtactgga cttccaccgg gactattgac tcttaacacc actgctttgc tattttttgc
     1741 ttcaaaggca tcggtcaaac taccaattaa atcatcggca ttggcatctg ccccatcggc
     1801 aatttcacct tttagttcaa caatcgcaac atggtcagca ctagattctg ttgctttacc
     1861 accatgctta tcgcctgatg tcaccattat caaaataacg actaaatacg taaaggttaa
     1921 taacttaaaa acgatccccc aacggcgtgc tttacgttgc tcatcaatcg aagctaaagc
     1981 aactttttct agcaattgcc attcacgacc attattttca ctcatattct ttcaatccta
     2041 aatgagataa tcttgtaact gataaatact atctactatt gccaacggtt gatagctttg
     2101 taacatatct ttactatgca caccataaga cacggcaatc cgaggcatgt ctagggcttg
     2161 agccatttcc atatcataac tggtatctcc aatcatgaca ctttgttcgg cactgctgcc
     2221 tgtatacgct aataactgac gcaacattaa cggatcaggc tttgatgcgg tttcatcagc
     2281 acaacgtgtg gcgacaaaat aatgaccaat acctgtttgt gacaatactc tatccaagcc
     2341 caagcgactt ttaccagtgg caattgccaa caaataacct tgttgctgca attgatctag
     2401 caaggactgt gcccctgtaa acaactcact tttactggca ctatgggcta aaaaatgttt
     2461 agcatactgt tggcgaattt cctctcgtgt actcagtgag gcatgaggaa ataacaaggc
     2521 aattgcttcg ggcaagccta aaccaataat atgacgaacc tctgatacac ttggcacagg
     2581 ttcacctaac acttgtgcgg cttgctgcat actgtcaaca atatgcgcca cagaatccat
     2641 taatgttcca tcccaatcaa aaatagcaag cttatacgcg ggcatttcgt aatttctcaa
     2701 gcacttgaac taattcgggg gctaaaggac agtttaattg caaaggcttt tcttgatttg
     2761 gcaattgtaa actgacatct tgcgcatgta aaaataaacg atttaagccc catttgtcct
     2821 tagctgtttt gttcaactca tttgagcaat acttttcgtc acccaaaatt ggatgaccta
     2881 aaaattgagc atgaacacgg atttggtgag tacggcctgt taatgggcgt gcctcaacta
     2941 aagtacaatc ttcaaaacgt tcaatcactt taaaatgggt ttcggcttct ttaccttcac
     3001 gcgatacacg taccacacgc tcacctgatg ccaaattatt tttatctaaa ggtgcagtga
     3061 ctttatgatt tgcgcctttt agcttacctg ccaataacgc taaatagacc ttttgcattt
     3121 tgccatcacg taattgctca tgcaaaacac gcaacacgct acgcttatca gaaatcatta
     3181 ataaaccaga tgtatctcgg tctaaacgat ggactaactc taaaaactcg gtttgtggat
     3241 aaatcgcacg taaggcttca atagccccat acgctacacc gctaccgcca tgtacaggta
     3301 aaccagaggg tttattaagg acaataatgc catcttgacg ataaataatg cgatttgcca
     3361 aggagttgga taactgctga ctaggcacaa ccacatcttc tttttcagcc aaacgcacag
     3421 gtggcacacg aacaacatca ccaatttgta aacgatattc aggtttaaca cgtcctttgt
     3481 ttacacgcac ctctcccgta cgcacgatac gatacactaa ggtgcgtggt gcgcctttta
     3541 acgcggtaat taaaaaattg tctaaacgtt ggccgtcttg attctcagta atggtaatca
     3601 atcgaacggc agggctagaa ttactatcag tcataaaaac aatcaacttg gataggaaag
     3661 tgtttaaaaa gagagcaaca aacaaataac aacaaactaa tcgtgtaaaa atagtgtgag
     3721 ctttaagcag agtctgctat agtcacacct gccgactgtt cgcgttgctt tagctaatga
     3781 ccacactaaa agacatggtg gcacattagc attcaataaa atcaatggcg agcggattat
     3841 ataggcaaat agtgtttttt gtatgggctt tttattgcga aaatgaactc gcaccccaaa
     3901 ttgcacacca agatatatat ttcacagacg ctttgtggtg tagtgaaaac agaaacgaat
     3961 tttttagtac tttctaccaa agcgtagaga tacttatacg tgtagtttat ccaactaacg
     4021 tgatgaccat attacactaa gactaaatct attatgtctt agctgaaaga gctttactga
     4081 ctaacagtgc gcttgcttat cagaaaattt ctagcaagag agtcattttg tcagtccgtt
     4141 taagacgaca catagtcaat aagtaaagtt ctcaagcaca cagcttgcct ttcgtttctg
     4201 tcctaccctc aaagtgtcac taaaattagg tgacaaccac tcatgaaacg tatgttaatt
     4261 aatgcctcac acgccgaaga aacgcgtgtt gcactggttg atggtcaacg cttgtatgat
     4321 tttgacctcg aacatcattc tcgtgtccaa aagaaagcca acatttacaa aggtcgcgta
     4381 acccgcgttg agccaagctt agaagccgct tttgttgact ttggtgcgga tcgtcatggc
     4441 tttttgccac tcaaagaaat tgctcgtgaa tattttcatc gtcaaccacg tgatgtgggt
     4501 cgtgtaacca ttcgtgatgc gattcaagaa ggccaagaaa tcattttaca agttgaaaaa
     4561 gaagagcgtg gcaacaaagg cgcagccctt tcaacattta tttctttagc tggtcgttat
     4621 ttggtgttaa tgcccaataa tccacgtgct ggtggtattt ctcgtcgtat tgaaggcgaa
     4681 gagcgcgaag aactcaaaga agccttaaac tccattacca tccctaatga tatgggcgtg
     4741 attgtccgta cggctggttt aggtcgtagc cccgaagaat tacaatggga cttagactat
     4801 ttattaacgt tatggaagtc gattagccaa gctgccgaac aaagtccagc accgttctta
     4861 atttatcaag aatctaatgt cattattcgt gcgattcgtg attatttacg tgatgatatt
     4921 gatgaagttt tgattgatag cgaagctgct tataatcaag cgattgattt tgttcgtcaa
     4981 gtgatgcctc acttccaaaa caaaatcaaa ctatatcgcg atactgtgcc gttgtttaat
     5041 cgctatcaaa ttgaaagcca aatcgaaacn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnna tggacagagc acgtgtacaa attggccgta tctctcgttt tggtttatta
     5221 gagttatctc gtcaacgttt gcgcccttct ttagaagaaa ccactggttt ggtttgtcca
     5281 cgttgtaatg gaacaggcgt gattcgtgac gtacgctctt tagcattgtc cattatgcgc
     5341 gtcattgaag aagaagtgtt aaaagatcgt agtgccgaaa ttcaagctca agtacctgtg
     5401 acggtagcaa cattcttatt aaatgaaaaa cgtgcagcga ttagtgcatt agaagcacgt
     5461 agtggagtac ggattattat tctgccaaat ccacacttag aaactcctca ttatgatgtg
     5521 tcacgtttac gtgatgatca tagtgatgat gattatgaag cgcccgcctc tttccaacag
     5581 gtcgaaaact tccgtcctgt tttagaagat aatggcttat ataacagcga agaaaagact
     5641 caaactcgcc gtcaagaagc tgcggtaaaa atggtacaac ctgaaactca agcacctgta
     5701 ccacgtgcca caacaccaac acaagtgatt acacgccaag aaaaagaaca gtcaaaaact
     5761 ggtttctttg catggcatgc taatttattt agtggtgtaa gtgaattagc taatgagatt
     5821 gatagcaaat tagccgtttc tcgtcccgca ccaagttata acaacagtga aaattttgtt
     5881 actaatgaca atgctcgctc tcaacgtcct caacgtgaaa accgtactaa tcgtgctgag
     5941 ccagaatttc gtatggaaag tcgtgacgtt gcaccaacac gccaaacacg tgtcgagatc
     6001 gaagcaactc cagcacctgt tcgcgaacca cgccctcaac gtgaacgtcc tcaacgtgta
     6061 gagcaagact tccgttcaga acatcatgtc aatgtttcac cacgtcaagt acgccataca
     6121 gaagtggttg aaccaattac accaccagta cgtgagccac gtccgcaacg tgaacgccct
     6181 caacgtgaaa accgtactca tcgtgctgat gctttagagg ttaattctgc tgcgttagcg
     6241 attgatgccc ctgttgcgcc tattgctgag caagaagaac aacgtcgtcc acgtcgtgaa
     6301 cgtccaagtg gtcatggctt taatcgtcgt caacgtccac cacgtgaacg tgatgctcaa
     6361 gttttagctg agaccacctt tgatgctact gtgccagaca gcaatgttac gccagttgct
     6421 tttgagccaa ttactgccga gttagtcact gctagtgatg acatgccagt cgttgttgag
     6481 cctgttagct ctcaagaaca agaaattgtt attgcgagtg aagcaactgt agaaatggct
     6541 ctaccagaag caaccgtaga gactagcaac gaggctcctg aacctgttgc agaaaataca
     6601 gcccaaacaa cagtgactcc tgttgatagc gaagtaataa cacctgaagc aacggtaaaa
     6661 gctgaagaag ttgttgcacc agtcattgag acaactgtta ttgaagcagc ccctactcgc
     6721 gcggcaaatg atcctcgtga gcgtcgtcgc cgtgagcgtg aggctgctga acgtgctgca
     6781 cgcgaagaag cagagcgttt agttgccgag caaacagcca aggaagctgc tgaacgttta
     6841 gccattgaac tcgctgcttt agcagcagaa caggctcaaa aaacgcaagc tacacctgct
     6901 gtaatgccag aagcaacttg gcgtactgtt ggtgagttta ttgctgcatt taccccagag
     6961 gttaaggcaa ttactggcgc ggaagttgtt agtgcgttta tggttgcaca aaagaaacag
     7021 caagaaatcg ctgctgccat tgctcaaaca gaagctactc ctcctgttgc cgaagaaaca
     7081 gcaactatca cggaagtttt agttgacact actgttgctg agccagttgt ggaggttatt
     7141 gcaaatagtg aagatgcttc tgctgctgtt agcttacctg tcagcaatac atggtacaca
     7201 gtcggcgaat ttattgctgc ttttattcct gacgccaagc ctataacagg cactgaaatt
     7261 gtcagtgctt ttatgcttgc tcagaaaaag caacaagagc taggctagta cgctgctaaa
     7321 cacacggtgt tatgtaacgc cgtgtgttta atcgtctatc aaaaataaat aaaaaagccc
     7381 atgaaaaatc atgggctttt ttatttatct ggcggagaga aagggatttg aacccttgat
     7441 aggagtataa cccctatgcc gaatttcgag tccggtgcat tcaaccactc tgccacctct
     7501 ccgtaaggcg atgtaatata tagatatttc tcccaacttt caagaaaaaa gaatctttat
     7561 aaaggaacat catctactgt atcggcagcc gtatcaattg tcgtatcagc tttttctaaa
     7621 gctgcatcta ctttatttcc tgcaacaggc acaacagaca ctactgcgcc aaccatactt
     7681 gtagttgcac ctacaacacg cattggcatg gtcactactt tagttaaaaa gcaaccgctc
     7741 aaactagaca ctaaaccaag cacaacaaca actttagcca tatttttcat ggttttgatt
     7801 ccattactca aagttaatgc ctaaacggcg acccacttct tcataagctt cgaccacttc
     7861 gcccaaacct tgacggaaac ggtctttatc taactttttg cgggtttctt tatcccacaa
     7921 acgacagcca tcaggtgaaa actcatcacc taagacaata cgatcatgga atacaccaaa
     7981 ctctaacttg aaatcgacta acaacatgtc gccttgcgca aataatgcct ttaatacatc
     8041 atttactgca aaagacaact ctttcatacg agctaattgt gcttcagttg cccagccaaa
     8101 agagcgaacg tggctttcgt taatcattgg atcgcccaat gcatcatttt tcaagaacaa
     8161 ttcaaaagtt ggtggattta actccaaacc ttcggtcact cctaaacggc gacataagct
     8221 acctgcggca tagtttctca ccacacactc aacaggaatc atggttaaac gcttaaccaa
     8281 cacctcatta ggcgtaatta atttctcgaa gtgggtttca atgcctgctt cagccaattt
     8341 ttgcataata aaggcattaa aagtgttatt aacacggcct ttacgcgcca attgctcaac
     8401 ttttttacca tcaaaagcag aagtgtcatc acgaaacact aaaactaaat aatcaggatt
     8461 atcagtggta tacacagact tagctttacc tgtatataat aattcacgct tttccatcga
     8521 aaattctcta catatatagt gatacttatt attagcctaa atgactaata aatataaaac
     8581 ttaacttggc caatgtttga ttaactcggt caaaatctgc tgacttaaca cactatctgc
     8641 taaagtatct tcatcttttt gcagggaaac aaccgtttta gcggctgttg aggttagttt
     8701 caacaaataa gcctctttct tgttatttgt ttgactgagt tcaataaaat atagaccaga
     8761 actttgattt ctgtcagtaa tttttacctt agcggcatta attgcctgac ccaattgctc
     8821 ccaaagcttt aactgctcac ctgttatcgt taaaacaggg tgctgattgc catcaaaagc
     8881 caaacttggt ttttgctcaa caatgatttg tgttttagac actgtttgtt cagattcagt
     8941 gaccaccaaa ggcttagggc ttggtgcaac aaattcctgt ttacggcctt taccctgtac
     9001 gatctgagcc gcttgctcag caggtgtagt gactacagga atttgatata aaggtttaat
     9061 actacgcgtt tggttgtcag taggtaatgg ctgaatactt tgcgcctgca aataatctaa
     9121 tgagcgatct ggaaataatg aacaaccact caacaacacc aaaccaaaac cagacaacca
     9181 taaacgcata ttaaatacct gcttgttgta aagcagcacg tacaacaggt tgacattgat
     9241 cagctaacac cgttaatggc aaacggatac ccaattcaat acgacccatt gcaaacaatg
     9301 cccactttac gggaatagga ttagactcaa caaataaatc tttatgcaag gcaactaatg
     9361 gtgcatttaa atcagccgcc gcttgggcat tacctgccaa tgctgcctca catacgtcgc
     9421 tcattgcttt tggtgcaaca ttggctgtca ccgagatatt gcccttcgca cccatcaaaa
     9481 ttaattgata agccgtcgca tcatcacccg aatacacagc cattttgtcg ccaaccaagt
     9541 ttaataactc ttggccacgc tctaaattac ctgttgcatc tttaatgccg ataatattag
     9601 gtgtacctgc caaacgtgcc actgtcgcat tagacatatc cacacccgta cgacctggca
     9661 cattatataa aatttgggga atatcgactg cctcagcaac ggctttatag tgctgatata
     9721 agccttcttg tgtgggttta ttgtaatatg gcgtgaccaa taaacaggca tccgcacctg
     9781 cttgtttcgc agagcgtgtt aattcaatcg cctcacgcgt agaatttgca cccgtacctg
     9841 caatcacagg aatacgttta ttaacagttt caacaacaca ttttatcact gcgcaatgtt
     9901 cttcaacatc taaagtcgcc gattcaccag ttgtgccaac tgctacgata ccgtgtgtac
     9961 cttgagcaac atgccattca actaatgact tcagggctga ccaatcgaca tcaccattgt
    10021 tgtgcattgg cgtaacaagt gccactaaac tgcccgtaat catgtgttat ctccgtcgtc
    10081 taccatcaga caattacaag atgcttatct tatttaatga atctctttaa aaacattcac
    10141 tacatgtaag cttactatta tatgccgtat cgctttgtaa gactaaaaat ttagtccaaa
    10201 acaaaaagcc gactaaaaag tcggcttttc aaaacttgga gcgggaaacg agactcgaac
    10261 tcgcgaccct aaccttggca aggttatgct ctaccaactg agctattccc gcgtttgtgg
    10321 atcgctattt tatgtatttt ttaggtcacg tcaagcaagt tttttatatt tccctttcgc
    10381 tcgcctactt tttacccaaa aaaccaaaaa agcttagaaa acaaacattt tcaaatccat
    10441 tttcttcaca atgagtgatt aatctgcttc actcaagtct ggccatgctg cttttaaata
    10501 aatcaacatc gaccaaattg ttaaaacaac cgcaatatac aacaaaacaa agcccaaata
    10561 atttaacgtc ggatctttaa ttaataggat ggtaatcgag gccatttgag ccgtggtttt
    10621 ccatttacca atttgcgaaa ccgcaacatt tgctcttttc cctaattctg ccatccattc
    10681 tcgcaaggca gaaat
//
LOCUS       DAOQMB010000035        10658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2395_length_10658_cov_5.942375, whole genome shotgun sequence.
ACCESSION   DAOQMB010000035 DAOQMB010000000
VERSION     DAOQMB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2395_length_10658_cov_5.942375"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="PLY05_02530"
     CDS             complement(<1..193)
                     /locus_tag="PLY05_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02207.1"
                     /translation="MMANDFDVITYGNGAFLYDVFNGVAAIFGNSSYGAAMATCMTAA
                     SIGIMISAVLQGRMVNLLWM"
     gene            complement(193..1746)
                     /locus_tag="PLY05_02535"
     CDS             complement(193..1746)
                     /locus_tag="PLY05_02535"
                     /inference="COORDINATES: protein motif:HMM:NF017892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HNC02208.1"
                     /translation="MKKWVMLGVFLMPMSVSNADMQSELQSWMSGDEFVNLNQPAAYQ
                     SQAAGYGVSFGGLKYRTTSIPVGGFASFRKPKLSAGCGGIDLDLGGFNFVNKDQIVQQ
                     LRAIASNAKGMVFQMAIDTVSSMLGNNMKAFKNVADFLNKGQMDSCAAASKMMSGITD
                     VVSPDIARNKCISQAIDERNLSYDQALRECGPQGARAGIIGDTTTQNRDSWKKGNLAW
                     AILMQDDYFKNNKENALLLMNITGTLIARDSIAGIETNTTDTNEQVVIKDYIQPWFIS
                     NTESGANCDDSQIKKGVGSVCYSKNVNRLLELLVNGKTTDGGMLNVFRCKDNNMVSDE
                     NGCPDVERDANGKPVYAALALAPDEAIKPKMSKLLKGIQVQMLLPNGSLTDEQKNFIG
                     EMDGAIYHYMLASSTLLRSANVEDSNLDAYLTLMAQRKVGERFAKITSNIRDLMTAGK
                     YSPDNKEDKQLYIKNLEKTIVAFGEIANTAKEGMIAMQQMQNMANLYEKALVSSMGAK
                     MLSRVKFGG"
     gene            complement(1776..>2252)
                     /gene="traF"
                     /locus_tag="PLY05_02540"
     CDS             complement(1776..>2252)
                     /gene="traF"
                     /locus_tag="PLY05_02540"
                     /inference="COORDINATES: protein motif:HMM:NF025105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HNC02209.1"
                     /translation="IWKTPEYDYTLEHPMRSDGIWANGDGRYEQVQNQFGAAAQKFGL
                     LYVFRSDCPYCKKFSPILKLFANTHGFSIISVSLDGQGSQDFPNPVKDLNLLRKRNAL
                     PEVVPALYLVDPRQPEAQALGFGLMNFNDLEIRVADAAGINMQQGTISESKINELQ"
     assembly_gap    2253..2352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2453..4498)
                     /gene="traN"
                     /locus_tag="PLY05_02545"
     CDS             complement(2453..4498)
                     /gene="traN"
                     /locus_tag="PLY05_02545"
                     /inference="COORDINATES: protein motif:HMM:NF018666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HNC02210.1"
                     /translation="MVNRFLVVCLCACNQVFADFASQNQQALDFATTGDIMATQEQLK
                     QGIDPNTDGGLGIGSMPCGQADANGKYYCGGQEITPTDNPPEAGFYHQAPSQLEDAGR
                     MKSVSDETAQFQMNTAIAMPVGLVKRDDPIFQFAEQNENALTPLSQSYTGCATIKGGA
                     NSGTNIFKTCSKTGVPNINHCYRSWTPKCTWINDDPMLINGSAGLPRVKETTYYAYDS
                     SGKVTKNTSGGTPFYHYTLARSTEFTLTINLRMINDGSILYFKSSHWVPNKGGCGDCG
                     IPSMTINGKTVDKASSYGISAKVGYHTVSFYNLKSYLVDGDNAVKITLTSDNNGANFD
                     TDIMFYNLYDIKECIKSITENFVCDGDMAKITAKPLVKTLCLDNENPKIVDGVAISRP
                     EGCWRQDEQHGFVGTGIFTEDSQCGQYRAEKCTALKEDCLVKHPEGWCANAKLTFSCS
                     SSTAEQTVEVCGETLICPDGNCYDQVKEQGDGTEGFKQVATYTEFLKNVKDNFNASDL
                     SVFKGQYKECSVNKTLIGSDSCCVGGSGTLNTLGKKCNGNEQQIADARNNKIVTFLGM
                     WEECGSKVLGVCVEKIIHYEYCVWPSKLARMLQDQGMPQLGQTITSACRGFKMQDPNE
                     FAAINFDLIDFSEYFADVTNKLNNTPMPNTDALSSQTQAAVPAMADKLNERYKDYGR"
     gene            complement(4492..5238)
                     /gene="trbC"
                     /locus_tag="PLY05_02550"
     CDS             complement(4492..5238)
                     /gene="trbC"
                     /locus_tag="PLY05_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HNC02211.1"
                     /translation="MLRSLKTMLFGLVLSSSSLVVLADEMARYQEPTQAEKNTAKNSI
                     AEQLKEAEAITQDADYQKNTARAVKQAQTMAVPNSMPIVNVRDEDVSSKNAIDIDKLT
                     ASHAGQTIEENRMTPLVLVSLSMPEQTLKGLTIEAARVGGSIAFRGAKEDSVMKMRNE
                     FARIGIEGQIDPSFFRRFNVDEVPTFIMPLERIKLCDSDNCTMSRHVKVSGLVSVETA
                     LEFISRNSQEDDAKAIAEQWLDKLRNTSTW"
     gene            complement(5223..6230)
                     /locus_tag="PLY05_02555"
     CDS             complement(5223..6230)
                     /locus_tag="PLY05_02555"
                     /inference="COORDINATES: protein motif:HMM:NF018536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HNC02212.1"
                     /translation="MKKRWSLLMLMTTLFLVNSGHAALGCAGRVLNPITDVCWLCFFP
                     MTIGGAKIGKGFDDWSGVSTPPPICQCPAPPPIFVRIGVGVTYWSPDRLTEVVKKPMC
                     SPSLGGKVLGKLPTTSGGVSGSDAVNRKSLGSFNHSHWMVMPLLQQLAMVVDTGLCMK
                     AETMDFMFLSEIDPLWNDDELSLIMSPESLLFSSLPAQVACAADSAKALVTNFGLDPL
                     FWCSGGQGSLYPLTGNKQFQDGAPDTALAMTHRMAFSLHRMGLEWDTSTVAAMCQNIP
                     QPLMRKGQYKSAFVYPVPSTKRAYGFGVPSILFHAGREFPYKGEEHTLMVWRRKTCCA
                     L"
     gene            complement(6227..6832)
                     /gene="traW"
                     /locus_tag="PLY05_02560"
     CDS             complement(6227..6832)
                     /gene="traW"
                     /locus_tag="PLY05_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HNC02213.1"
                     /translation="MKRLLLVVLLVCSNGQAEDLGKISQTYKIAENDLAEVFKMRVAK
                     AVQNGTWAKEMDKQAKKIQAYVDNPTGMRLPIARHYNARYFDPSMILEQDIVDQNNRV
                     IVPKGTRVNPLDRISYNKALCFFDANDTKQIEWIKSMCLDLVTAKAIAINGPVMKVME
                     ELDARLFFDQQGILTKKFGIKAVPSMVRQTGNVFVIEEFAL"
     gene            complement(6792..7322)
                     /gene="lepB"
                     /locus_tag="PLY05_02565"
     CDS             complement(6792..7322)
                     /gene="lepB"
                     /locus_tag="PLY05_02565"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC02214.1"
                     /translation="MTLTQEQQQAIKAWRLSKFRTQQGRLATVLFLLAAIYMAISQFA
                     FLAVKVTNSIEGKLFVIWVGEQPKRYDLVMFKPKQHLIEAIGLAQWTKGVCGVAGDVV
                     THQNRTVFVNGKKVGDAISYSKLYQSNMELIDDMVIPEGFVFVCGTHAQSFDSRYKLV
                     GLVDEKTFIGRATRLF"
     gene            complement(7319..7744)
                     /locus_tag="PLY05_02570"
     CDS             complement(7319..7744)
                     /locus_tag="PLY05_02570"
                     /inference="COORDINATES: protein motif:HMM:NF021213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI F-type domain-containing protein"
                     /protein_id="HNC02215.1"
                     /translation="MTTNDIEKPVALEADKAAGTNALWVQLLLVIFVSLMVFVAMRGI
                     EKVFFDKEIIAVDLKVVLSKELEKSGTQIMTEEQRNQRASAFNQALEVALDKVSNNGH
                     KIILVSPAVISGVTDYTQVVTDEVTRTLDESFKQRGFQK"
     gene            complement(7741..10281)
                     /gene="traC"
                     /locus_tag="PLY05_02575"
     CDS             complement(7741..10281)
                     /gene="traC"
                     /locus_tag="PLY05_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HNC02216.1"
                     /translation="MSFFKKLMQQFVEKDVGNDYGKSFLPPTKARIKSTSATAKVSDL
                     FSYRRYDQSKGLYFNHDSVGFALICAPATGLNIEQLRTLETIFTTQYRADTMLQVSLV
                     ADPNVGDLLSIWEQGRTKGNENHDIFASMAKGRVDYLKKASWASLFSEEVVMIRDYHL
                     VITMNMPYREGQLELSATQLDELERNKDTVIGALKSASIIAEVMTPSILINLYGNLFR
                     PELTQKATRLKVNYDPRMPINEQIVSNETAVYVNRDSLVIKYQDNYVSCLPYSVKKFP
                     VEWIGSANGELTGAFFNRIQRIACPFMITMCIHIPDQTSAAATAQNNMLRATQMKDTE
                     VGKYVPQWKGRHADWTYVVGCMEQGSKLIRASYQIVLFAPQGTEQACEQSLRNVFSAQ
                     GWNLVKDRFCTVPRVMSAMPLWIGRENYELMNKLKFFSTFLSWNAVNLAPWIGEWKGN
                     VPYGQDPMLMFLGRRGQVCYLDPFLNTKGNFNIAVAAASGAGKSFFTQDYVKAILGSG
                     GKAFIIDSGRSYENLCQILDGEYIDFGEGNNICLNPFTTILEDPVAGGNNFSEQLPLL
                     IALIGTMASPMEPISAKHNAILGKAILSAWQSFGNKTTMTQVQKALQKSPEQEGHDLS
                     IMLSPYCIDGAYEQYFEGEGNINFKKNFVVLELEALNAKADLQIVVIFLLMKRITEAM
                     YLSGRGQRKICIIDEAWRLLGSGNSAAVIEEGYRVARKYNGSFMTVTQSLMDYYKAPT
                     ATAAYVNSDFVFLLRQKPESLAEVEMKGYLVMTPFEKRVYGSITTFGGRYSEIAIKSP
                     DGLAVGQLVVDKFSGKLMSTKAEDVHAIKMFEQQGYSKLDAIKKVALA"
     gene            complement(10296..>10658)
                     /locus_tag="PLY05_02580"
     CDS             complement(10296..>10658)
                     /locus_tag="PLY05_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02217.1"
                     /translation="IYHLRCLPFDEHSKISSLLPLLTQNDEFVITANYWCDKDGLLWG
                     QLIVIVLATENIDDRLKNIFFGRGWLIEQECAHPILLFLAALPMGLSAFNLQLLQEHN
                     RFMRLEHHEAMRIMPTLV"
BASE COUNT         3020 a         2445 c         1898 g         3195 t
ORIGIN      
        1 acatccataa caaattcacc attctgcctt gcaaaacggc actaatcata atgccgatgc
       61 tggctgccgt catacaagtg gccattgctg caccgtaact actattacca aaaatagccg
      121 ctacaccatt aaatacatcg tacagaaacg cgccattacc ataggtaatc acatcaaaat
      181 cattagccat cattagcccc caaactttac tcttgagagc atttttgcgc ccatgctact
      241 gactaaggct ttctcatata aattggccat attttgcatt tgttgcatgg cgatcatgcc
      301 ttctttggcg gtattggcaa tttccccaaa agcgacaatg gtcttttcta agtttttaat
      361 atataactgt ttgtcttctt tattgtcggg gctatattta cccgccgtca tcaagtctct
      421 aatgttactt gtaatttttg caaatcgttc gccaaccttg cgttgtgcca ttaaggttaa
      481 atatgcgtct aaattgctat cttctacatt tgctgagcgt aatagcgttg aactcgccaa
      541 catataatgg taaatggctc cgtccatctc cccaataaag tttttttgtt catccgttaa
      601 tgagccattt ggcaataaca tctgtacttg aatgcccttt aataatttag acattttagg
      661 ctttattgcc tcgtcaggtg ctaaagccaa agctgcatac acgggcttgc catttgcatc
      721 acgctcaaca tcaggacaac cattttcatc actgaccata ttattgtctt tacaacgaaa
      781 gacattcagc ataccgccat ctgtagtttt tccattgact aacaactcta ataagcgatt
      841 gacatttttt gaataacaaa cagagcctac ccctttttta atctgactat catcgcaatt
      901 cgcacctgac tcggtatttg aaataaacca cggctgaata tagtctttaa tcacaacttg
      961 ttcgtttgta tccgtcgtat tagtttcaat tcctgcaata gagtcccttg caatcaatgt
     1021 ccctgtgata ttcattaaca gtaaggcatt ttctttattg tttttaaaat aatcatcttg
     1081 catcaaaata gcccaagcaa gattgccctt tttccatgaa tctctatttt gcgtcgtagt
     1141 atcaccaata atgccagccc tcgccccttg tggcccacac tctcttaatg cttggtcata
     1201 agataaatta cgttcgtcta tcgcctgaga aatacattta tttcttgcaa tatcaggaga
     1261 gacaacatca gttatgccac tcatcatttt tgatgctgcg gcacaagaat ccatctgccc
     1321 cttatttaaa aagtcagcga catttttaaa ggctttcatg ttattaccga gcatacttga
     1381 cacggtatca atggccattt gaaagaccat gccttttgca ttactggcaa ttgctcgtaa
     1441 ctgctgaaca atttgatctt tatttacaaa gttaaaaccg cctaagtcta agtctatacc
     1501 tccacaacct gcacttaatt tgggtttacg aaacgatgca aaacccccaa caggaataga
     1561 agtcgtacgg tattttaaac ccccaaaact aacgccatag cctgccgctt gcgattgata
     1621 agcagctggt tggttcagat tgacaaattc gtcaccactc atccagcttt gcaattcact
     1681 ttgcatatct gcattagaaa cgctcattgg cattaaaaat acgcctagca ttacccattt
     1741 tttcattcgt cattcctatt tatcaatgtt taggactatt gcagttcgtt aattttagat
     1801 tcggagattg tgccttgttg catattgata cctgcggcat cggcaacgcg aatttcaaga
     1861 tcattaaaat tcatgagacc gaaccctaaa gcctgtgctt caggttgtct tggatcaact
     1921 aaatacaatg ctggtacaac ctctggcagt gcattacgtt ttcttaatag gtttaaatct
     1981 ttaacaggat tgggaaagtc ttgtgaacct tggccatcca gtgaaacact gataatggag
     2041 aagccatgtg tattggcaaa tagttttaag atgggcgaaa attttttgca gtaagggcaa
     2101 tcactccgaa aaacgtataa caagccaaac ttttgagcag ccgcaccaaa ttgattttgt
     2161 acctgttcat aacgtccatc accatttgcc catataccat cgctacgcat gggatgctct
     2221 aaggtataat cgtattcagg tgttttccaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nntcaggttt gggcgggtct tggtaataaa tccagctttt tggcgtatcg
     2401 taggccaata cactcatact aaaaaaaatt gataggcaaa acattaacgc tttcatctgc
     2461 catagtcctt atatcgttca ttgagtttat cggccatcgc gggaacagca gcctgtgttt
     2521 gactagacag cgcatcggta ttcggcatgg gggtgttatt gagtttgttg gtgacatcgg
     2581 cgaaatattc actaaaatct attaaatcaa agttaatagc cgcaaactca tttggatctt
     2641 gcatcttaaa accacggcaa gcactggtga tggtttgccc taactgtggc ataccttgat
     2701 cttgtaacat tcgcgccaat ttgcttggcc atacgcaata ttcatagtga ataatttttt
     2761 cgacacacac acccaaaact ttactgccac actcctccca catccctaag aaggtgacga
     2821 ttttattgtt tctcgcatca gcaatttgtt gctcattgcc attacatttt ttacccaagg
     2881 tattgagcgt gcctgaacca ccaacacaac aactgtccga accaatgagg gttttattaa
     2941 cagaacactc tttatattgc cccttaaaga ccgacaaatc agaggcgtta aagttatctt
     3001 tgacgttttt aagaaattcc gtataggtcg ctacttgttt aaagccttct gtaccatcac
     3061 cttgctcttt aacttggtca taacaattgc catcaggaca aatcaaggtt tcgccacaaa
     3121 cctctaccgt ttgctcggct gttgagctgg aacagctaaa agtgagctta gcgttggcac
     3181 accaaccttc aggatgctta accaaacaat cttcttttaa agctgtacat ttttcggcac
     3241 gatactggcc acattgagag tcttcagtaa aaatacctgt cccaacaaag ccatgttgct
     3301 cgtcttgtct ccaacagcct tcgggtctag atatagcaac accatcaaca attttgggat
     3361 tttcattatc taaacaaagg gttttgacca atggtttggc ggttattttg gccatatcac
     3421 catcacagac aaaattttca gtaatgcttt tgatacattc tttaatgtcg taaaggttat
     3481 aaaacataat atccgtatca aaatttgcac cgttattatc tgatgttagg gtaattttta
     3541 ccgcattatc accgtcaacc aaataactct ttaggttata gaatgagaca gtgtgataac
     3601 caactttagc ggaaatgcca taagagcttg ctttatctac cgttttacca ttgatagtca
     3661 ttgatggaat gccacaatca ccacaccccc ctttattggg aacccaatgc gaagatttaa
     3721 aatagagtat cgagccatca tttatcattc gcagattgat agtcagcgta aactctgtac
     3781 ttcttgctaa ggtgtaatga taaaaaggcg taccaccact ggtgttttta gtcacttttc
     3841 cgcttgagtc ataagcgtaa taagtcgttt ctttgacacg aggtaagcct gccgagccat
     3901 tgattaacat tggatcatcg ttaatccatg tacatttagg agtccatgag cgataacagt
     3961 gattgatatt cggcacacct gtcttgctgc aagttttaaa aatatttgta ccgctattcg
     4021 ccccaccttt aatcgttgca caacctgtat aactttgtga taagggagtt aaagcatttt
     4081 cgttttgctc agcaaattga aaaatcgggt catctctttt gactaaaccc acaggcatcg
     4141 caatcgcggt attcatctga aattgggcgg tttcatcact gactgacttc atgcgtccag
     4201 catcttctaa ctgactaggg gcttgatgat aaaaacccgc ttcgggtgga ttatcagtgg
     4261 gcgttatttc ttgaccacca caataatact taccattcgc atccgcctga ccacaaggca
     4321 tggagccgat tcctaaacct ccgtctgtgt ttggatctat tccttgcttg agttgctctt
     4381 gtgttgccat gatgtcgcct gtggttgcaa aatcaagggc ttgttgattc tgactagcaa
     4441 aatcagcaaa cacctgatta cacgcgcaaa ggcaaaccac taaaaaacga tttaccatgt
     4501 gcttgtattc ctcagcttat ctaaccactg ctccgcaatg gctttggcat catcttcttg
     4561 actattacgt gaaataaact ctaaggctgt ttcaacgctc actaatcctg acacttttac
     4621 atggcgactc attgtgcaat tatcactgtc acacaacttt atccgttcta aaggcataat
     4681 aaatgttggc acctcatcaa cattaaatcg tctaaaaaaa gaaggatcta tttgaccttc
     4741 aatgccaata cgcgcaaact cgttacgcat tttcatcaca ctatcttctt tggctccgcg
     4801 aaaagcgata ctaccgccta cacgagctgc ttcgatggtt agtcctttaa gcgtctgttc
     4861 aggcatagac agactaacta aaactaatgg cgtcatacga ttttcttcaa tagtctgccc
     4921 agcatgactt gccgtaagtt tgtcgatgtc tattgcattc ttggaagaaa catcttcatc
     4981 tctgacatta acaatcggca ttgaattggg cacagccatt gtttgtgcct gtttaaccgc
     5041 tctagccgtg tttttttgat agtctgcatc ttgagtgatg gcttcggctt cttttaattg
     5101 ctcagcaata ctatttttgg ccgtattctt ttcggcttgt gttggctcct gataccttgc
     5161 catttcatca gcaaggacta ccaaactcga tgatgacaaa acaagtccaa ataacatcgt
     5221 ttttaaagag cgcaacatgt tttacgtctc caaaccatca aggtgtgttc ttcaccctta
     5281 taagggaact cgcgtccagc gtgaaataag atactaggta caccaaaccc ataggcacgt
     5341 tttgtactag gtacgggata cacaaaagca gatttatact gccctttacg cataaggggt
     5401 tggggtatgt tttgacacat ggcggcaacg gttgaggtat cccactctaa gcccatacga
     5461 tgtaatgaaa atgccattct gtgggtcatg gcgagtgcgg tatcaggcgc accatcttga
     5521 aactgtttat tgcctgttaa tggatacaaa ctcccttgcc cacctgaaca ccaaaacaag
     5581 ggatctaagc caaaattagt cactaaggct ttagccgaat cagcggcgca agcaacttgt
     5641 gcaggtaatg aggagaaaag tagcgattcg ggcgacataa ttaacgatag ctcgtcgtcg
     5701 ttccataatg ggtctatttc ggataaaaac ataaaatcca tggtttccgc tttcatgcac
     5761 aaacctgtat caaccaccat cgctaattgt tgtaataacg gcattaccat ccaatgcgaa
     5821 tgattgaaag agcctaatga ttttcgatta acggcatcac taccacttac cccaccactg
     5881 gtggtgggta atttacctaa tacttttccg cccaaacttg gcgaacacat cggctttttg
     5941 acaacttcag tcagtctatc gggcgaccaa taagtcactc ctacccctat gcggacaaaa
     6001 ataggcgggg gtgctggaca ttgacaaata ggtggaggcg ttgatactcc actccagtca
     6061 tcaaaacctt tgccaatctt tgctccacca atcgtcatcg gaaaaaagca caaccagcaa
     6121 acatctgtaa tcggattaag cactcgcccc gcgcaaccta atgccgcatg accactattc
     6181 accaagaata atgttgtcat caacattagt aatgaccatc gttttttcat aaagcaaatt
     6241 cctcaatcac aaacacatta cctgtttgac gaaccatcga gggaacagcc ttaatcccaa
     6301 attttttagt taaaataccc tgttgatcaa aaaagagtct tgcatctaat tcctccatca
     6361 ccttcatcac aggcccattg atagcaatcg ctttagcggt cactaaatca agacacatac
     6421 tcttaatcca ttcgatttgt ttggtatcat tggcatcaaa gaagcacaac gctttgttat
     6481 aactaatccg atctaaaggg ttaactcgtg tacctttagg cacaatgaca cgattatttt
     6541 gatcaacaat atcttgctct aagatcatag aggggtcgaa atatcttgca ttgtagtgcc
     6601 gtgcaatcgg caagcgcata cccgttgggt tgtcaacgta ggcttgtatt ttcttggcct
     6661 gcttatccat ttctttagcc caagtcccat tttgaactgc cttagcaaca cgcattttga
     6721 agacttccgc taagtcattt tctgcaattt tataagtttg cgaaattttg cctaagtctt
     6781 ctgcttgccc attagaacaa acgagtagca cgaccaataa aagtcttttc atcaaccagt
     6841 cctactaatt tgtatcgaga atcaaaggat tgcgcatgag taccgcacac aaaaacaaat
     6901 ccttctggaa tcaccatgtc atcaattaac tccatattgc tttggtataa ttttgagtag
     6961 ctaatggcat cgccaacttt tttaccattc acaaaaacgg ttctgttttg gtgtgtcacc
     7021 acatcaccag cgaccccaca tacgcccttt gtccattgtg ctaatccaat agcctcaatc
     7081 aaatgctgtt tgggtttaaa catgactaaa tcgtatcttt taggctgctc gcccacccaa
     7141 attacaaaca gctttccttc gatactgtta gtgactttga ctgctaaaaa agcgaattgt
     7201 gaaatggcca tataaatggc tgccaataga aataacactg tggctagtcg tccttgctgt
     7261 gtacgaaact tacttaatcg ccaagcctta atcgcttgtt gttgctcttg cgttagggtc
     7321 atttttgaaa gcctctttgt ttaaagcttt catcaagtgt gcgcgtcact tcatcagtaa
     7381 ccacttgcgt ataatcagtt acgccactaa taacagcagg cgacactaaa atgattttat
     7441 gtccattatt tgatacttta tctaaggcga cttctaaggc ttgattaaat gctgatgctc
     7501 gttggttacg ctgttcttca gtcataatct gcgttcccga tttttctagc tccttactca
     7561 gtactacttt taaatccacg gcaataattt ctttatcaaa gaaaactttc tcaatgccgc
     7621 gcatggcgac aaacaccatg agactcacaa atatcactaa cagtaattgc acccaaagcg
     7681 catttgtccc tgctgcttta tctgcttcca gtgcgactgg tttttcaata tcgtttgtcg
     7741 tcatgcgagt gccacctttt tgattgcgtc taatttgcta taaccttgtt gttcaaacat
     7801 cttgatggca tgaacatctt ctgccttcgt tgacattaac ttgccactga atttatcaac
     7861 caccaattgc ccgaccgcta aaccatcagg gcttttaatg gcaatttctg aataccgccc
     7921 accaaaggtg gtaatagagc cataaacacg cttttcaaaa ggggtcatca ccaaatagcc
     7981 tttcatttca acttcggcta aggactcagg cttttgacgt aataagaaaa caaaatcact
     8041 gttaacgtag gcggctgttg cagtaggggc tttgtaataa tccatcagag attgggtgac
     8101 tgtcataaag ctgccgttgt atttacgagc aacgcgataa ccttcttcaa tcacagccgc
     8161 actattacca ctgcctaata aacgccacgc ttcgtcaata atgcagattt tgcgttgtcc
     8221 cctgcctgat aaatacatcg cttcggtaat acgcttcatc agtaaaaaga taacgacaat
     8281 ctgcaaatcc gctttggcat taagggcttc taactctaaa accacaaaat ttttcttaaa
     8341 gttgatattt ccttcacctt caaaatactg ttcatatgct ccatcaatgc aataaggtga
     8401 caacataatg ctgaggtcgt gtccttcctg ttcaggcgat ttttgtagtg ctttttgaac
     8461 ttgtgtcatg gtggttttgt taccaaaact ttgccatgca cttaaaatgg ctttgccaag
     8521 aatggcatta tgcttggcag agataggctc catgggtgaa gccatcgtgc caattaacgc
     8581 aatcaacaga gggagttgtt ctgaaaagtt atttccacca gcaacggggt cttctaagat
     8641 agtggtaaat gggttcaaac aaatgttatt accctcgcca aaatcaatgt attcaccatc
     8701 taatatttgg caaagatttt cgtaactacg gcctgaatca ataataaaag ctttaccacc
     8761 actgcccaaa atggctttta catagtcttg ggtaaaaaag cttttacctg cgccacttgc
     8821 ggctgccacg gcgatattaa aattgccctt ggtatttaag aatgggtcaa ggtaacaaac
     8881 ttggcctcgc ctgcctaaaa acatcagcat cgggtcttgg ccatagggga cattgccttt
     8941 ccattcacca atccaagggg ctaaattaac ggcattccag ctcaaaaacg tagaaaagaa
     9001 tttaagctta ttcatcagct catagttttc tcggccaatc cataaaggca tggcactcat
     9061 gacacgcggc acagtgcaga atctatcttt gaccagattc catccttggg cagagaacac
     9121 attacgcaaa ctttgctcac aagcttgctc ggtgccttgt ggggcaaaca acacaatttg
     9181 ataactggct cgaattaact ttgaaccctg ctccatacaa ccgacaacat aagtccaatc
     9241 tgcgtgccta cccttccatt gcggcacata tttaccgact tcggtatctt tcatttgcgt
     9301 cgcacgcagc atattatttt gagcagttgc agccgccgat gtttggtcgg ggatatgaat
     9361 acacatcgtg atcataaagg ggcaagcaat acgttgaatg cgattaaaaa acgcccctgt
     9421 cagttctcca ttagcagagc caatccattc cactggaaat ttctttaccg aataaggcaa
     9481 acaactgaca tagttatctt gatatttaat gactaagcta tcacgattga catacacagc
     9541 ggtttcatta gatacaatct gctcattgat tggcattcgt gggtcatagt taacttttaa
     9601 tcgcgtcgct ttttgagtaa gttctggtct aaataaattg ccataaagat ttatcaaaat
     9661 actgggggtc attacttcag cgataatact tgccgacttt aatgcaccaa tcaccgtatc
     9721 tttatttcgc tcaagctcat ctaattgtgt cgctgataat tcgagttgtc cctctcggta
     9781 aggcatattc atggtgatta ctaaatggta atcccgaatc atcaccactt cttcgctaaa
     9841 caacgaagcc catgacgctt ttttcaaata atcaacacgt ccttttgcca tgctggcaaa
     9901 aatatcgtgg ttttcattgc ctttggttct gccttgttcc caaattgata ataaatcgcc
     9961 aacattagga tctgccacaa gactcacttg caacatagta tccgcacggt attgagtcgt
    10021 aaaaatcgtt tctaaagtac gcagttgctc gatgtttaat cctgttgcgg gggcgcaaat
    10081 taaggcaaaa ccaacgctgt catgattaaa atacagtcct ttactttggt cataacgacg
    10141 atagctaaac aggtcactga ctttagcggt cgctgaggta cttttaatcc gcgctttggt
    10201 tggaggtaaa aaagattttc cataatcatt accaacatct ttttcgacaa actgctgcat
    10261 cagcttctta aaaaagctca taaatcattc cttgcttaaa ccagagttgg catgatacgc
    10321 atcgcttcat gatgctctaa acgcataaag cgattatgtt cttgtaataa ctgaagatta
    10381 aatgcggaca gtcccatagg taatgctgct agaaaaagca aaattggatg ggcgcactct
    10441 tgctcaatca gccaaccacg tccaaaaaaa atatttttta agcggtcatc gatattttcc
    10501 gttgctaaca caataacaat caattgcccc cataacagac catccttgtc acaccaataa
    10561 ttagcggtaa tcacaaactc atcgttttgc gttaatagcg gtaataacga ggaaattttg
    10621 ctatgttcat caaaaggtaa acatcgtaag tgataaat
//
LOCUS       DAOQMB010000036        10623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2404_length_10623_cov_19.361090, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000036 DAOQMB010000000
VERSION     DAOQMB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2404_length_10623_cov_19.361090"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="PLY05_02585"
     CDS             complement(<1..1175)
                     /locus_tag="PLY05_02585"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNC02218.1"
                     /translation="MYQPLAERMRPQNLDQVCGQQHLLAEGAPLRILLTQGQCPSLVF
                     WGSAGVGKTTLARLIAKYVDAHFIALSAVLAGVKELREAISEAQQHSRGLLAKRTILF
                     IDEIHRFNKSQQDALLPYVEDGTITLIGATTENPSFELNAALLSRLRVLVLKPLSEDD
                     LLQILQAALTDQQRGLAAQADSLPSHWLKTIAQFADGDARKALVLLETAYELSRAQGT
                     DDKVLTLFLGKGLRRFDKGGDQFYEQISALHKSVRGSNPDAALYWLSRMLDGGADGDY
                     LARRLLRMAIEDIGLADPRAQQLALEAWQTYERLGSPEGDLALANVAVYLACCAKSNA
                     VYSAFKEVKQVIEQTGSLEVPIHLRNAPTKLMKELGFGAGYRYDHDENGHAVGQQFLP
                     "
     gene            complement(1202..1651)
                     /gene="fur"
                     /locus_tag="PLY05_02590"
     CDS             complement(1202..1651)
                     /gene="fur"
                     /locus_tag="PLY05_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HNC02219.1"
                     /translation="MTFSNQDLRKAGLKVTLPRIKILELLENSPVHHLSAEDVYKALL
                     EQGEDVGLATVYRVLTQFESAGIVERHHFEGGHSVFEISKDEHHDHIVCMTCGKVLEF
                     HDELIEAQQHKVAESLGFTLTGHALHLYGICSNPECQEKTNGRKMLK"
     gene            1754..2182
                     /gene="bamE"
                     /locus_tag="PLY05_02595"
     CDS             1754..2182
                     /gene="bamE"
                     /locus_tag="PLY05_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HNC02220.1"
                     /translation="MSAKLYLIALLPSLLLLPACSTLHNLKNSLSVYKIDLPQGNPIT
                     QDMANKLKIGMTPSQVRYVLGSPMVTDTFNANRWDYVYRFTAGTYAKAAGINSVTTPH
                     LSLRFDNGKLSEIKGIDTLPTEAPANILSKDGGLRAEPLN"
     gene            complement(2246..2542)
                     /locus_tag="PLY05_02600"
     CDS             complement(2246..2542)
                     /locus_tag="PLY05_02600"
                     /inference="COORDINATES: protein motif:HMM:NF002490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HNC02221.1"
                     /translation="MTEKLLTVEVAYALPQQQKIISLQVPPQTTLFEAILLSKIASYF
                     PEINLDTLSVGVFSKIEKSPKTRLVQQGDRIEIYRPLLVDPKEARKARAAKLQL"
     gene            complement(2539..3585)
                     /locus_tag="PLY05_02605"
     CDS             complement(2539..3585)
                     /locus_tag="PLY05_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02222.1"
                     /translation="MNQSLSAISAVSLITISLLHATGLAGHALHLSFMPVWLLFMTII
                     ALPLTFIEAAIVRRTQQLPLQALVPMTRDADAKTTWRLLPWLAGLVLILLIGYTAQYP
                     KQYIASWAQQAVLKESISYLLIFFAIGFAWVGMRRLLKYVGVLVPIALIVNGLSAKYN
                     FQIQLLTAEQWQMVAMATLLTSVLTLGVYAWLILVQPQSTHASRSVLPLWLTQTVIGL
                     LVLTIGHTSGQLYVASYMFCAVFAVAIFADVIAAQLQLKSIQKPIAFGGVLLLGIVAC
                     YLAEYQIFDTVLQSLSLLTMILYCVFVGWVMKISHVRKALNFSHEGIYNLWRVAIRLV
                     VPLTSLWLLLSLFI"
     gene            complement(3593..5533)
                     /locus_tag="PLY05_02610"
     CDS             complement(3593..5533)
                     /locus_tag="PLY05_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNC02223.1"
                     /translation="MTQVVRTGDKLLTGIQAFSAWKLSIANEIKRYRLWLHEQGLSSV
                     DLDDRLARALRAFTTDRILLAFVGEFSRGKTELINAILGRHYKMRLFPTRIGRTTMCP
                     TEVFYDPQANGPYIKLLPIQTRSSATPLASYRRQLEHWAHLDLDLTNTQSIQRAFEEV
                     AKTREVTIQAAESLGFEIEFLESSSKKAGYVHIPAWRHALINIDHPLLRMGLSIIDTP
                     GLNALGLEPELTLSFLPEAHALLFVLSADCGVTASDFSIWNNHVKDLAARSNTALYAV
                     INKIDLLEDEDSLYPIDVQEALNRLVRFSAQQLHLPMNNVLPISARQGARSFVNNNPQ
                     LYDSSRLSLLEEALATSVVSARQKALKNVTLKEVIEIVDNSLHTLTEKRDALQKEFDL
                     FNRNEKDVEVELVGLTRLVRKEQEKHNQRLVLLQEGKGALEQHWGRVNELVGNERLDS
                     HFVRAKASVTGAVAFGAAAAIGVFFQGLKLDLRRLRDEMDNATQTAGKIYQRYAQEFQ
                     QHPAQVPLIDIEQYISEFTELEKQAAPYKNRFGSLLANQEQVFDHFFDTLAREAKTVY
                     EKAREEAVRWCKQVLVPLMQQTLEAKKRIEEQLKRLDHLQMIDATRDTRLIEIQHQLE
                     ELKHQENFLQEVSFCLQPVELS"
     gene            complement(5505..5831)
                     /locus_tag="PLY05_02615"
     CDS             complement(5505..5831)
                     /locus_tag="PLY05_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HNC02224.1"
                     /translation="MSVITQQKEGYVLTLHIQPNAKKTEIIGVHGDALKVRLQAPPVD
                     GKANDALCAFFAEIFAVRRQSVVLLSGETSRQKRILIKNATTLPTLIIKNCKEFIGDS
                     GGANRG"
     gene            complement(5831..6397)
                     /locus_tag="PLY05_02620"
     CDS             complement(5831..6397)
                     /locus_tag="PLY05_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HNC02225.1"
                     /translation="MGAQIADLLIRTVFSLAISVFWLRFLLQLVRADFYNPISQWIIR
                     VTSPVLNPIQQVIPVYKGWHLGALGFIFLLQLVSMNLIALLKGLGTLPILPLLLGAVI
                     QLLYLATEFYFWLLLISVVLSWISPGYSPFGALIAQLAEPALAPFRRLLPPMGGLDLS
                     PIVAFLAIQIIQIVLGHFVQTLAKMGVL"
     gene            complement(6401..7219)
                     /gene="proC"
                     /locus_tag="PLY05_02625"
     CDS             complement(6401..7219)
                     /gene="proC"
                     /locus_tag="PLY05_02625"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNC02226.1"
                     /translation="MKIGFIGAGNMASALVGGLLAKGLNPKSIALADMHLPQLQRFAD
                     QGMFTTTRNEEVIERADIVILSVKPQVMAEVLKPLATIIQQKKPLIISIAAGINVDSL
                     SRWLGGDLSIVRAMPNTPALVQTGATGLYASPLVSFEQRQQTETVLGAVGLTLWVDDE
                     ALIDSVTAVSGSGPAYFFYVMEAMISAGKALGLDEKTARALTLQTALGAAQMAITSDE
                     PPAELRKRVTSKGGTTERAIATFDEANMQQIFAQALAACAARGQELATILGQQE"
     gene            complement(7245..7931)
                     /locus_tag="PLY05_02630"
     CDS             complement(7245..7931)
                     /locus_tag="PLY05_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNC02227.1"
                     /translation="MQQISQQLQQVIQRVNQACDKVGRNPDEVQLLAVSKKQDCTAIR
                     AAFEAGQCHFGENYVQEALEKMQQLSDLDITWHLIGALQSNKTLEVAESFAWVHSVDR
                     LKIAQRLSKQRPNHLVPLNICLQVNIDDQQSKSGCQLNEVESLVKEILLLPRLSLRGL
                     MVIPQAGNHQAFEQLANLRQQLLATIPNLSAQVFDTLSMGMSDDLEQAIAAGSTMVRV
                     GTAIFGKRLS"
     gene            8052..9086
                     /locus_tag="PLY05_02635"
     CDS             8052..9086
                     /locus_tag="PLY05_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003084552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HNC02228.1"
                     /translation="MDITELLAFSAKNGASDLHLSAGLPPMIRVDGEVRRINLPAMEH
                     KDVHRLVYDIMNDKQRRDYEEFWETDFSFEIPGVSRFRVNAFNQNRGAGAVFRTIPSK
                     ILSMDDLGMGKVFKDVSDFPRGIVLVTGPTGSGKSTTLAAMIDYINSTRFDHILTVED
                     PIEFVHQSKKSLINQREVHRDTRGFNEALRSALREDPDVILVGEMRDLETIRLALTAA
                     ETGHLVFGTLHTTSAAKTIDRVVDVFPAEEKDMVRAMLSESLQAVVSQTLLKKNGGGR
                     VAAHEIMLGTPAIRNLIRENKIAQMYSAIQTGGAIGMQTLDQCLKNLVGKGLISPAVA
                     RSAAKIPENF"
     gene            9100..10227
                     /locus_tag="PLY05_02640"
     CDS             9100..10227
                     /locus_tag="PLY05_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNC02229.1"
                     /translation="MEFEDLLKLMVEKGGSDMFITAGVPPSIKLNGKIVPITKSPLAP
                     DMAREVVMSIMSEDQRLEFQRTKECNFAISARGIGRFRVSAYYQRNLAGMVLRRIETK
                     IPSVDDLKLPEIIKDLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNANSRGHIITIED
                     PIEFLHTHQGCIVSQREVGLDTESFEIALKNTLRQAPDVILIGEIRTREVMDFAIAFA
                     ETGHLVLATLHANNANQALDRIIHFFPADRHGQLFMDLSLNLRGVVAQQLVPTPDGKA
                     RRACIEVLLNTPLISDYIRKGEVHLLKELMKKSRELGMQTFDQALYDLYSSGEITYED
                     ALKHADSPNDLRLMIKLGNNSDARHLDQATRSLSIQGSDDH"
     gene            complement(10278..10511)
                     /locus_tag="PLY05_02645"
     CDS             complement(10278..10511)
                     /locus_tag="PLY05_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNC02230.1"
                     /translation="MLRCRELVEQTDAYLADDLTKWQRIQFRLHLAVCRYCRRYVRQL
                     KTTQKVSQQQVLMMQTTDQQVEDIWTKLQQQDK"
BASE COUNT         3267 a         2309 c         1918 g         3129 t
ORIGIN      
        1 ggtaaaaatt gctgaccaac agcatgtcca ttttcatcat gatcataacg ataacctgca
       61 ccaaaaccta gttctttcat caatttagtc ggggcattac gcaaatgtat gggtacttct
      121 aaagagcctg tttgctcaat gacttgttta acttctttga aggcgctata cactgcatta
      181 ctttttgcac aacaggctaa atataccgca acattggcta aagccaaatc accctctgga
      241 ctccccaaac gctcatacgt ttgccatgcc tcaagagcta attgttgtgc gcgtggatct
      301 gccaagccta tatcttctat cgccatgcgc aacaaacgtc gtgctaaata atcgccatct
      361 gcaccaccat ctaacattct gcttaaccag tacaaagcgg catctggatt agaaccacgt
      421 accgatttat gcaaagccga aatctgctca taaaattgat cgccaccttt atcaaaacgc
      481 cgtaagccct tgcctaaaaa tagtgttaat actttgtcat cagtgccttg tgcgcgagat
      541 aactcataag cggtttctaa taagactaag gctttgcgcg catctccatc ggcaaattga
      601 gcaatcgtct ttaaccaatg gcttggtaaa ctatctgctt gtgcggctaa accacgttgc
      661 tgatcggtca aggcagcttg caaaatttgt aacaaatcat cttcacttaa gggttttaat
      721 accaatactc ttaagcgcga caataacgca gcgtttaatt caaaggaagg attttcggta
      781 gttgcaccaa ttaaagtaat cgtgccatct tcgacatacg gtaataaagc atcttgttga
      841 cttttattaa aacgatggat ttcatcaata aataaaattg ttcgcttagc caataatccg
      901 cgtgaatgtt gttgcgcttc gctgatcgct tcacgcaact ctttaacacc agccaatacc
      961 gcagacaagg caataaaatg agcatcaaca tacttagcaa ttaaccgagc taaggtggtt
     1021 ttgcctaccc ctgcactgcc ccaaaatact aaagaaggac actgcccttg tgttaacaaa
     1081 atgcgtaaag gcgcaccttc ggctaataaa tgctgctgac cacaaacttg atctaagttt
     1141 tgtggtcgca tacgttctgc taagggttgg tacattaaac tttaccccct taattggtag
     1201 gtcatttaag cattttacgg ccattggttt tttcttggca ttcaggatta ctacaaatac
     1261 catataaatg caaagcatga cctgttaagg taaaccctaa tgattcagca actttatgtt
     1321 gctgcgcttc aatcaactca tcatgaaact ctaaaacttt gccgcaagtc atacaaacga
     1381 tatgatcatg atgttcgtct ttactaattt caaataccga atgaccacct tcaaaatgat
     1441 ggcgttcaac aatacccgcc gactcaaatt gagttaacac acgatacact gtggccaaac
     1501 caacatcttc accttgctct aataaagctt tataaacatc ttcagcactc aaatgatgta
     1561 caggtgagtt ttctaatagc tctaaaattt ttatacgggg caaagtcacc tttagacctg
     1621 ctttgcgtaa atcttgatta ctaaatgtca tgactgagcc tctttggtgc taaaaaacaa
     1681 cagatttctt aaaagaaaat agtgtaggat tatcaacgaa taaaaagcga tgaacaagag
     1741 aggaaaactt ttcgtgagcg caaaactgta tctaattgct ttattaccta gtttactatt
     1801 attacctgcc tgtagcactt tacataatct taaaaactct cttagtgttt ataaaattga
     1861 tttgccacaa ggtaatccga ttacacagga tatggccaac aaactgaaaa ttggcatgac
     1921 tccatcccaa gtgcgttatg tattaggcag tccaatggta acagatactt ttaatgctaa
     1981 tcgctgggat tatgtttatc gttttaccgc tggcacttat gctaaagcag caggtatcaa
     2041 ttccgtgaca acaccacacc tcagtttacg ctttgataac ggtaaactca gtgaaattaa
     2101 gggcattgac accctaccca cagaagcacc agccaatatt ttaagtaaag atggtggctt
     2161 acgagcagaa ccattaaatt aggttttaac ttaggacttg ggcgatatca aacagttgaa
     2221 cgtaagtaca cgactatagc ctaagctaca gttgtagttt agccgctctt gctttacgtg
     2281 cttcttttgg gtcaactaat agtggacgat aaatttctat acgatcacct tgttgcacca
     2341 aacgtgtttt gggtgatttt tctattttac taaacacacc tactgagaga gtgtctaaat
     2401 taatttcagg aaaataactg gctatttttg acaagagtat cgcctcaaac aaagttgttt
     2461 gtggtggtac ttgtaaagaa atgatttttt gctgttgagg taacgcatag gctacctcaa
     2521 cggtcaataa tttttcggtc atataaacaa actaagcaat aaccatagac ttgttaatgg
     2581 tacaaccaaa cgtatcgcaa cacgccatag attataaata ccttcatggc taaagttgag
     2641 cgctttacgt acatgactaa ttttcatgac ccatccaaca aagacacagt ataaaatcat
     2701 cgttaataaa cttaaacttt gtaagaccgt gtcaaatatt tgatattcag ctaagtaaca
     2761 agcaactatg cctaacaata gcacgccacc aaaagcaata ggtttttgaa tacttttcag
     2821 ttgcaattga gccgcaataa cgtcagcaaa aattgccaca gcaaatactg cacaaaacat
     2881 ataactagca acatacaatt ggcctgatgt atgaccaatc gttaacacca acaaaccaat
     2941 aactgtttgc gttaaccaca atggtaatac tgaacgtgag gcgtgtgtgc tttgaggttg
     3001 aactaaaatc aaccacgcat agacgcctaa ggtcagtact gatgttaata aggttgccat
     3061 tgctaccatt tgccattgtt cagccgttaa taactgaatt tgaaagttat attttgcaga
     3121 taatccattc acaattaacg caattggtac taaaacaccg acatatttta gcaatcgacg
     3181 catacctacc catgcaaaac caatggcaaa aaagatgagt aaatacgaga tactttcttt
     3241 taaaacagct tgttgcgccc atgaagcaat atattgttta ggatactgag cggtataacc
     3301 aattaataaa attaatacta aacccgctaa ccaaggcagt aaacgccatg tagttttagc
     3361 atccgcatca cgcgtcatgg ggactaaagc ttgtaatggc aattgttggg tacgacgcac
     3421 tattgctgct tcaataaagg ttaatggcaa agcaatgatg gtcataaata atagccatac
     3481 aggcataaaa cttaaatgga gtgcatgacc tgctaaacca gtagcatgca ataaactaat
     3541 cgtaatcagt gacactgcac tgattgctga taaagattga ttcataaaaa aattaactca
     3601 attcaacggg ttgtaagcag aaagaaactt cttgcaaaaa gttttcttga tgctttaatt
     3661 cctctaattg atgctggatc tctatgagac gagtatctct tgttgcatca atcatttgta
     3721 aatgatccaa acgttttaat tgttcttcaa tgcgcttttt ggcttctagt gtttgctgca
     3781 tcaatggtac taaaacttgt ttacaccaac gcactgcttc ctcacgtgct ttttcataaa
     3841 cagtttttgc ttcgcgtgcc aaggtatcaa aaaaatgatc aaaaacttgc tcttgattgg
     3901 ccaataaaga accaaaacga tttttataag gagccgcttg tttttctaat tcagtaaact
     3961 ctgaaatata ttgttcaata tcaatcaatg gcacttgtgc aggatgctgt tgaaattctt
     4021 gggcataacg ctgatagatt ttacctgccg tttgcgtggc attatccatt tcatctcgca
     4081 aacgacgtaa atccaatttt agcccctgaa aaaatacccc tattgcagct gcggcaccaa
     4141 atgcaacagc ccccgttact gatgctttag cacggacaaa atgtgaatct aatcgctcat
     4201 tgcctactaa ctcattgact cgcccccaat gctgctccaa tgcgcccttt ccttcttgca
     4261 acaagactaa acgttgatta tgcttttctt gctctttacg cactagacga gttaaaccaa
     4321 caagttcaac ctcaacatct ttttcattac gattaaataa atcaaactct ttttgtagtg
     4381 catcgcgctt ttctgtcaga gtatgcaagc tattgtcaac tatttctatg acttccttaa
     4441 gtgtcacatt ttttagggct ttttgccgag cagatacaac agacgttgct aaagcttcct
     4501 ctaataatga taagcgacta ctgtcatata actgaggatt attattcaca aaggagcgcg
     4561 cgccttgacg cgcactgatg ggtaaaacat tattcattgg caaatgtaat tgttgtgcag
     4621 aaaagcgcac taacctattt aaagcctctt gaacatctat tggatataaa gaatcttcgt
     4681 cttctaataa atcaatctta ttaataactg catatagtgc ggtgttacta cgcgccgcta
     4741 aatctttaac atgattattc caaattgaaa agtcactagc tgtgacacca caatctgccg
     4801 ataacacaaa caacaacgca tgagcttcag gcaaaaaact taaggttaat tcaggctcta
     4861 aacccaaggc atttaaacct ggtgtatcaa tgattgataa tcccattctt aataatggat
     4921 gatcgatatt aattaaagca tgacgccatg caggaatatg gacatatccc gcttttttgg
     4981 atgatgattc taaaaattca atttcaaaac ccaaactctc agcagcttgg atagtgactt
     5041 ctctggtttt tgccacttct tcaaaagcac gttgaatgga ttgggtattg gttaaatcta
     5101 aatctaaatg cgcccaatgt tctagctgac gacgataaga agccaagggt gtcgcactac
     5161 tacgtgtttg aatgggtaat aatttaatat aaggcccatt agcttgagga tcataaaaca
     5221 cctcagttgg acacattgtt gtgcgtccaa ttcgggttgg aaacaagcgc attttataat
     5281 gacgacctaa aatcgcatta attaactcag ttttaccacg cgaaaactct ccaacaaaag
     5341 caagtaagat acgatcggtc gtaaaggcac gtaatgcccg agccaaacga tcatctaaat
     5401 caaccgaact caagccttgc tcatgtaacc ataaacgata acgtttaatt tcattggcaa
     5461 tcgacagctt ccatgcgcta aatgcttgta ttcctgtgag gagcttatcc cctgttcgca
     5521 ccacctgagt caccgatgaa ctccttgcaa ttttttataa tcagagtagg aagagtagtg
     5581 gcatttttta tcaatattcg tttttgccga ctggtctcac cgcttaacaa tacaacagac
     5641 tgtcgtctca ctgcaaaaat ttctgcaaaa aaagcgcata acgcatcatt cgctttacca
     5701 tcaacaggag gggcttgcaa acgcactttc agtgcatctc catgtacccc tataatttct
     5761 gtttttttag cattgggctg aatatgtaat gttaatacat aaccttcttt ttgttgagtg
     5821 ataacactca ttacaaaacg cccatttttg ctagcgtttg cacaaaatga cctagaacga
     5881 tttgaattat ctgtatcgct aaaaatgcaa cgatgggtga taaatctaaa ccacccatag
     5941 gtggcaataa acgtctaaaa ggtgccaacg ctggctctgc taattgtgca attaacgcac
     6001 caaatggcga ataaccagga ctaatccaac ttaaaaccac actaattaat aacaaccaaa
     6061 aataaaactc agttgccaaa tacaataatt gaatcactgc gcccaataac aaaggcaaaa
     6121 taggcaaagt ccctaatcct ttcagcaaag caatcagatt catgctgacc agttgcaata
     6181 aaaaaatgaa tcctaatgcg cctaaatgcc accctttata aacgggaatc acctgttgga
     6241 tgggattgag tacaggtgaa gtcacacgaa taatccattg cgaaattgga ttataaaaat
     6301 ctgcacgtac taactgcaac agaaagcgca accaaaaaac actaatggcc aagctaaaga
     6361 cggtacgaat cagtaaatct gctatttgtg cacccatgtt ttactcctgc tgtcccaata
     6421 tcgttgctaa ttcttgacca cgggctgcac aagccgctaa agcctgagca aaaatttgct
     6481 gcatattagc ttcatcaaaa gttgcaatgg ctcgctcagt tgtgccacct ttagaggtaa
     6541 ctcgttttct tagctcagca ggaggctcat cagacgtgat cgccatttgt gctgcaccta
     6601 atgcagtttg tagggttaag gctcttgccg ttttttcatc taaacctaat gctttacctg
     6661 cactaatcat cgcttccatg acataaaaga aatatgctgg cccagaaccc gatactgctg
     6721 taacgctatc aattaatgct tcatcatcaa cccataatgt caaaccaaca gcacctaata
     6781 ctgtttctgt ttgttgacgt tgctcaaaag acactaaagg actggcatat aggcctgttg
     6841 cacctgtctg taccaatgca ggcgtattag gcatagcacg aactatactt aaatcaccac
     6901 ctaaccaacg actcaaacta tcgacgttaa tgcctgccgc gattgaaata atcagtggct
     6961 ttttttgttg aataatcgtt gctaaaggtt ttaatacctc agccatcact tgcggtttaa
     7021 ctgataaaat cacgatatca gcacgttcta tgacctcttc attacgagtt gtcgtaaaca
     7081 tgccttggtc agcaaaacgc tgtaactgcg gcaagtgcat atctgccaaa gcaatagatt
     7141 tggggtttaa gcctttagct aataatccac caaccaatgc agaagccata ttacctgcac
     7201 caataaaacc tatcttcatc gtcatttatt tcctaattta aaatttagga cagccgttta
     7261 ccaaaaattg ctgttcccac tcgcaccatc gttgatccag ccgcaatcgc ttgttccaaa
     7321 tcatcggaca ttcccattga tagtgtatca aacacttggg cagataaatt gggaattgta
     7381 gcgagtaatt gctgacgtaa attagccaat tgctcaaaag cttgatggtt tcccgcttgt
     7441 ggaatcacca tcaaaccgcg tagagataaa cgtggcaata ataaaatctc tttaaccaaa
     7501 gattctacct catttaattg acaacctgat ttactctgtt gatcatcaat attaacctga
     7561 agacaaatat tcaagggtac taagtgatta gggcgttgtt tagataatcg ttgagctatt
     7621 tttagccgat caacactatg cacccatgca aaactttctg ccacttccaa ggtcttatta
     7681 ctttgtaatg caccaattaa atgccaagta atgtctaaat ctgataattg ctgcattttt
     7741 tctaaggctt cttgaacata attttcacca aaatgacatt gccctgcctc aaaagccgcc
     7801 ctaatcgctg tgcaatcttg ttttttacta acggctaata attgcacttc atcgggattt
     7861 cgtcctactt tatcgcaagc ttgattgact cgctgaataa cttgctgcaa ttgttgagaa
     7921 atttgttgca ttctgacact atctcattca aagaattacg ttactattaa cttatgattc
     7981 gtaaataaaa actcacatgc tcattgctat gcaatacgcc tgttttatat ataacaatat
     8041 tggagctaac aatggatatt accgaattat tagccttctc cgcgaaaaat ggcgcatctg
     8101 acttacatct ttctgctggc cttcccccaa tgattcgtgt tgatggtgaa gttcgccgta
     8161 ttaatttgcc tgcgatggaa cataaagacg tgcatcgttt ggtctatgat attatgaacg
     8221 ataaacaacg tcgtgattat gaagaatttt gggaaacaga cttttctttc gaaattccgg
     8281 gtgtttctcg ttttcgtgtc aatgctttta accaaaaccg cggtgcaggt gcagttttcc
     8341 gaaccattcc ttccaaaatt ttaagtatgg atgatttggg catgggaaaa gtttttaaag
     8401 atgtaagcga ctttcctcgt ggtattgtct tagtgacagg tccaacaggc tctggcaagt
     8461 caaccacttt ggccgcaatg atcgactata ttaacagtac tcgttttgat cacattctga
     8521 ccgttgaaga ccctattgaa tttgttcatc aatctaaaaa aagcttgatt aaccaacgcg
     8581 aagttcaccg tgatacacgc ggctttaatg aagcattgcg ttcggcacta cgtgaagacc
     8641 ctgacgttat tttggtgggt gagatgcgtg acttagaaac cattcgtttg gcattaactg
     8701 ctgccgaaac aggtcactta gtctttggta cattacatac aacctctgct gctaaaacca
     8761 ttgaccgtgt ggttgacgtt ttccctgccg aagaaaaaga catggttcgt gccatgttat
     8821 ccgaatcgtt acaagctgtt gtatcacaaa ctttattgaa gaaaaatggt ggtggtcgtg
     8881 tggctgccca cgaaattatg cttggtacac ctgcgattcg taacttgatt cgtgaaaata
     8941 aaattgcgca aatgtactcc gcgattcaaa caggtggcgc aattggcatg caaacccttg
     9001 accaatgttt aaaaaatcta gtcggtaaag gtctcatttc accagcagtc gctcgttctg
     9061 cggctaaaat tcctgaaaac ttctaagtgg agaaacataa tggaatttga agacttatta
     9121 aaactgatgg tggaaaaagg tggttctgac atgttcatta ccgctggtgt gccgccatca
     9181 attaaattaa acggaaaaat cgttccgatt actaaaagcc ctctcgctcc tgatatggct
     9241 agagaagtgg tgatgagtat tatgagcgaa gatcaacgtc ttgagtttca acgaactaaa
     9301 gagtgtaact ttgcgatctc tgctcgtggc attggtcgct ttcgtgtcag tgcttattat
     9361 cagcgtaacc ttgctggcat ggtattacgc cgtatcgaaa ccaaaattcc ctctgtagat
     9421 gatttgaaac tgcccgaaat cattaaagat ttagccatga ccaaacgtgg cattattatc
     9481 tttgttggcg caacaggtac tggtaaatcc acttcattag cggcgatgat tggccatcgt
     9541 aatgccaata gccgtggaca tatcatcacc atcgaagacc cgattgagtt tttacatacc
     9601 catcaaggtt gtattgttag tcaacgtgaa gtaggtttag atactgaatc cttcgaaatt
     9661 gccttaaaaa acaccttgcg ccaagcacct gatgtcattt tgattggcga aattcgtacc
     9721 cgcgaggtga tggactttgc cattgccttt gctgaaacgg gtcatttggt attagccacc
     9781 ttacacgcca ataatgctaa ccaagcactc gatcgtatta ttcacttctt tcctgctgat
     9841 agacatggcc aattgtttat ggatttatcc ttaaacctca gaggggttgt tgcacaacaa
     9901 ttagtcccaa cacctgatgg gaaggctcgc cgtgcatgta ttgaagtctt gctgaataca
     9961 ccattaatta gtgattatat ccgtaaaggt gaagtgcatt tgctcaaaga gttaatgaaa
    10021 aaatcgcgtg aattagggat gcaaaccttc gaccaagcgt tatacgattt atattcttct
    10081 ggtgaaatta cttatgaaga tgcattaaag catgctgact caccaaatga tttacgatta
    10141 atgattaaac ttggcaataa tagcgatgct cgccatttag atcaggcaac acgtagtttg
    10201 agtattcaag gctctgacga tcattaacct caagctccaa tagctgttga cgttttcaaa
    10261 acgccaacag cccatactta cttgtcttgc tgttgtaatt tagtccaaat atcctctact
    10321 tgctgatctg tggtttgcat cattaatact tgttgctgac tgactttttg agtcgttttt
    10381 aattgccgta cataacgccg acaataacga cacacagcca aatgcagacg aaattgtatc
    10441 cgttgccatt tcgtcaagtc atcagccaaa taagcatctg tttgctctac aagctctcga
    10501 cacctcaaca tagcagacct tcctcatgtt tgttaatgac cgctaataat tttagtctag
    10561 ccctatgcat tgacatcctc cccgccctca atacttcgac taagctcagt aaccgagggc
    10621 gag
//
LOCUS       DAOQMB010000037        10414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2468_length_10414_cov_14.516749, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000037 DAOQMB010000000
VERSION     DAOQMB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2468_length_10414_cov_14.516749"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..2664
                     /locus_tag="PLY05_02650"
     CDS             187..2664
                     /locus_tag="PLY05_02650"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HNC02231.1"
                     /translation="MSNLFNNYTQRPDCYNEIFATDGTIHPHWQRFASVLQGMTAEQV
                     QARADLVAQQIYENGVTYNVYGDEQGLNRPWRLGVIPNIIPYDEWQVLAEGIEQRAKV
                     LNAILADLYGEQRLVKEGLIPAELVYGHNNFLWPCIGIKQPHDLFLHQYAADVARDVN
                     GQWWVMADRTQTPSGAGYALENRQIIARVFPELYRQLKVQSLNDYFLILRQTIGRLAP
                     TTGEQPLIVLLTAGRFNETYFEHIYLARHLGIPLVEGYDLTVRGSMVYLKTLNGLKRV
                     HAILRRLDDDYCDPLELRSDSALGVAGLLDAVRSGNVLVANALGSGVVESAGLLGFLP
                     KICQYLFDEPLKLPSVPTWWCGEQPVLEDALDKLPQLVVKPTFPSQRFEPVFAQDLDD
                     EQLQTLKQRIARRSYTYVAQQQVPLAQSPVWQPKTKQFLAQASGMRVYAVASDEGYRV
                     MTGGLARVSSDSQASVVSMQRGGLSKDVWVCFGQSARSERPKVRTIGVRDLLRQDPYL
                     PSRVAENMFWLGRYSERCDNNARLLRSALSRHIELGGESDLALTIALDSCQFLALLPA
                     KEDIGQQLLTAICDNKIGNSLVANLRSLIWSASQVRGRLSQENWITIAELQQEADNLN
                     PNKLELGKALGFVDRLLMSLSALSGFALDDMTQDNSWRFLMIGRRLERLQFLADMIAQ
                     LLQHDAATEQTALDWLLELADSTITYRSRYLSSPQLIPVLDLILLDPSNPHSLQFQLD
                     SLSQYLRQLDDSNDYGLSQMSERLQSLNFAVLESEMDYIQRLPRALMLIAKLLKSIAE
                     QGRQLSDQLNLRYFAHIETFSQPTVSA"
     gene            2664..3563
                     /locus_tag="PLY05_02655"
     CDS             2664..3563
                     /locus_tag="PLY05_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNC02232.1"
                     /translation="MKTARYQITHETHYRYAAPVSLSQQLLHLQARAMPYQACLQQEL
                     NIQPEASDSQALEDSFGNPYTRLAFNQPHDELLVVSTMTIEVLPRQLADFKNSMPWQK
                     VVDVCRYQGQQTYSSELVNALPYRFQSPYIPLKHQFADYAASCFDKDKPLLLAVQDLM
                     HKIFKEFTFDPKATHIGTPLLEVLEKKRGVCQDYAHFMIACLRSLGLPTRYVSGYLLT
                     HPPEGQPRLIGADASHAWLAVYCPILGWVDFDPTNNLIPSTEHITMAWGRDFSDVSPL
                     RGVILGGGSHDLDVRVTVMPITE"
     gene            complement(3567..4073)
                     /locus_tag="PLY05_02660"
     CDS             complement(3567..4073)
                     /locus_tag="PLY05_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HNC02233.1"
                     /translation="MVDLKFLRAYSPTLQNEVQLLIAQGKLETYLNTRYPISTHQIQT
                     DKSLYEFTVNIKQNCLKNAPNLDKVCYDNKLDVVHHALGLHTAVSRVQGSRLKAKKEI
                     RIASLFKQTPADFLTMIVAHELAHLKEPTHNKAFYQLCQHIEPNYHQLEFDLRLFLTY
                     QELKNTSN"
     gene            complement(4189..5700)
                     /gene="lnt"
                     /locus_tag="PLY05_02665"
     CDS             complement(4189..5700)
                     /gene="lnt"
                     /locus_tag="PLY05_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005255385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNC02234.1"
                     /translation="MKSLLSKLRHSKFAPIYAGILGALLPFSLAPYHCWPLALVCIAL
                     FADLLPQQTPKQALLRGWSFGTGLWGVGVSWLFVSIHEYGYTPAWLASLMVLFVAIVM
                     GSFTGLMAYLYRRFNLDKHALLTFAPLFIIFEWLKTWLFTGFPWLFTGYAFIDSVLVA
                     YAPILGVFGVGLFAVVSAQCLLMIAKYQQKAWSYAALISLIWGVGYGLQYVTWTKSDY
                     QQGLSVSIVQGNIPQDVKWQMEWRDKTLAIYEQLSKSEWGQDVVIWPEAAIPMFQYEA
                     NDFLIKINNTATATNTAFVTGIPYADLENINKQGIPPFYNSIAALGAGNGLYFKQRLV
                     PFGEYVPFESWLRGVLPFFNLEMSSFSAGNPQQSSLIVKDHKLAAAICYEIAYPEITR
                     RNAEKADFITTLSNDGWFGTSTGPHQHLQMVQMRALETGKWIIRATNTGISGFISPKG
                     TLVKKAPQFKRTVLRGHVYPVKGQTPFSRLGNWPILIFCFGILIISFWRCRRI"
     gene            complement(5700..6551)
                     /locus_tag="PLY05_02670"
     CDS             complement(5700..6551)
                     /locus_tag="PLY05_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNC02235.1"
                     /translation="MSDERGNDTPKSWFNRLSNLLSTEPENREELLAIIHSAGKRGVL
                     DAESQQIIESTLRVADMQVREIMVPRAQIMSIRDDSEPKDFLPILIESAHSRFPVLAS
                     DNSDEVVGILLAKDLLRLIGQKSDRFDLKDYLRPAFCVPESAHLDRLLREFKEKQTHM
                     AIVVDEYGGIAGLVTLEDALEQIVGDIHDEHDDADEGSHWISEQADGTYVVQAITPIT
                     EFNDYFGSQFSHEDFETIGGIILSEFARMPQINEYIDLVHWRFTILAADLRMISVIRV
                     QDLRKIT"
     gene            6675..6902
                     /locus_tag="PLY05_02675"
     CDS             6675..6902
                     /locus_tag="PLY05_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02236.1"
                     /translation="MNKLMTVLVCVLTLVACQKQEPVKTTEDTGRPETRSIEAADAMG
                     YNGKEVRKKVDGALDANDAHNAQLEQQTNEQ"
     gene            complement(6904..10362)
                     /gene="mfd"
                     /locus_tag="PLY05_02680"
     CDS             complement(6904..10362)
                     /gene="mfd"
                     /locus_tag="PLY05_02680"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004738938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNC02237.1"
                     /translation="MTAVSTLTTKLLLPTHTGDKKIWANLLGSGAGLSLSLAIQQWSG
                     VVLLITADSQQAARLEEEVRFFSGEQIPILHFPDWETLPYDLLSPHQDIISERLRILA
                     TLPTLTKGLVITPITTLAQRLAPPSWLSAQSFSLKCGQKFDLDATRQRLEAVGYRAVE
                     TVYEHGEYAVRGSLMDIYPMGATAPVRIELFDDEIDSLRLFNPDNQRTTETIEQLELL
                     PAREFPFDRTAIRLFKDQWAETFPQTDPKKCALYQDINQGLAAAGIEYYLPLFFKEHA
                     SLCDYLPRHSLVATMSDCHSQLDHFWTELNQRYEDRRHDNHLPILSPDSLFLRSNELF
                     GQLNNFARLQLNQQNEAEKAGTSLFDFSQPINLPIDSRSDTPLAHLASYIQQQPQRIL
                     LCAESAGRRESLLSLLNKANLQPILVNSWQEFIEKDLRFALTIAPLEQGLSLPDVTVI
                     AESQLFGQRVMQKRRRKATTGEGAAELAIRSLSELTIGVPVVHIDYGVGRYQGLITLD
                     MDGQTQEFLQLIYADNAKLYVPVANLHLIARYSGSEDALAPLHKLGNEQWQKARKKAA
                     EQVHDVAAELLSISARRAAKAGYAFKFDEQEYAKFCAGFAFEETADQANAILSTIADL
                     RSPRPMDRLVCGDVGFGKTEVAMRAAFVSIHDGKQVAVLVPTTLLAQQHYENFCDRFA
                     DWPVKIEVLSRFKTAKEQQKALDELATGKIDIIIGTHKLLQDSVEFKDLGLMIVDEEH
                     RFGVRHKEALKNRRADVDLLTLTATPIPRTLNMALSGLRDLSIIATPPAKRLAVKTFV
                     RESHNNVIKEAILRELLRGGQVYFLHNDVATIEKCAADLQGLLPEARVGIAHGQMRER
                     DLEHVMQQFYHKQFNVLVCSTIIETGIDVPSANTIIMERADKLGLAQLHQLRGRVGRS
                     HHQAYAYLLIPNKKLLSADAEKRLEAIANADDLGAGFALASQDLEIRGAGELLGEEQS
                     GNMQAVGFTLYMEMLERAVKAIKAGKTPNLEQPLSLTAEINLRLSALIPDDYLPDVHN
                     RLLMYKRISHAESYEALDELQVEMIDRFGLLPEATKNLFATHKIKLQAADLGIKKIDA
                     HAQGGRLEFAKDTTVQPITIVQLVQKQPAQFKLDGAEKLRFISDLTDSQKRLSFVEQL
                     LTKLKS"
BASE COUNT         3090 a         2060 c         2136 g         3128 t
ORIGIN      
        1 aagcgacaag cctaagtgtt aaccccgacc atcctttcat gttagactta cgccgtaaat
       61 aatacatagg ctgtatagct cttcaatctt aggacttacg cacttgtaag tcctaagtct
      121 tggtgataaa agctctcttc aagagcattt tcgcagagta tcgcccacta aattttgtcc
      181 cctagtatgt ctaatttatt taataattac actcagcgtc ccgattgtta taacgaaatt
      241 ttcgcaacag acgggacaat tcatccccat tggcaacgtt ttgccagtgt cttgcaaggt
      301 atgactgctg aacaagtaca agctcgcgcc gatttagttg cccaacaaat ttatgaaaat
      361 ggtgtgacgt ataatgttta tggcgatgaa caaggtttaa atcgtccgtg gcgtttaggg
      421 gttattccta acattatacc ttatgatgaa tggcaggttt tagcagaagg cattgaacaa
      481 cgtgccaaag tcctcaatgc aattttggca gatttgtatg gtgaacagcg tcttgttaaa
      541 gaaggtttaa ttcctgcgga actggtttat ggtcataaca attttttatg gccatgtata
      601 ggtatcaaac aaccccatga tttatttttg catcaatacg cggctgatgt tgcgcgtgat
      661 gtcaatggtc aatggtgggt gatggcagat cgtacccaaa cgccatcagg tgcaggttat
      721 gccttagaaa atcgacaaat tattgcgcgg gtctttccag agttataccg tcaactaaaa
      781 gtgcaatcgc taaatgatta ttttttaatt ttacgtcaaa ccataggtcg tttagcacca
      841 accacaggcg aacagccatt aattgtcttg ttaaccgcag gtcgatttaa tgaaacttat
      901 tttgaacata tttatttagc tcgtcattta ggcattccat tggttgaagg ttatgattta
      961 acggtgcgtg gtagcatggt gtatttaaaa accttaaatg gtttaaaacg tgtacatgcc
     1021 attctacgcc gcttagatga cgattattgt gatcctttag agttgcgtag tgactctgcg
     1081 ttaggggtag caggattatt agatgcagtg cgctcaggta atgttttagt tgctaatgct
     1141 ttgggttcag gcgtggtgga gtcagcagga ttattaggct ttttacccaa aatttgccaa
     1201 tatttgtttg atgaaccttt aaaattaccc tctgtgccaa catggtggtg tggtgagcaa
     1261 cctgttttag aagatgcttt agacaaatta cctcaattag tggttaaacc cacatttcct
     1321 tcacaacgtt ttgagcctgt ctttgctcaa gacttagatg acgagcaact ccaaacatta
     1381 aaacagcgta ttgctcggcg atcttatacc tatgttgctc aacaacaagt gcctttagcg
     1441 caatcgcctg tttggcaacc aaaaactaaa caatttttgg cacaagcctc aggtatgcgt
     1501 gtctatgcgg tagccagcga tgaaggttat cgagtgatga cagggggctt agcgcgtgta
     1561 tcttccgata gtcaagcctc ggttgtttct atgcagcgtg gtggcttgag taaagatgtt
     1621 tgggtatgtt ttggccagag tgcgcgcagt gagcgaccta aagtccgtac cattggtgta
     1681 cgagatttat tacgccaaga cccttatttg ccctcgcgtg ttgccgaaaa tatgttttgg
     1741 ttaggacgat attcggagcg ttgcgataat aatgcccgat tattaaggtc tgctttgtca
     1801 cgccatattg aattaggggg cgaaagtgat ttagctctca caattgcctt agatagttgt
     1861 caatttttag ccttattgcc cgcaaaagaa gatattggtc aacaactatt aaccgccatt
     1921 tgtgacaaca aaataggcaa tagtttggtg gcaaatttac gcagtttaat ttggtcggcg
     1981 tcacaagtgc gtggccgttt gtcacaagaa aactggatta ccattgccga gttacaacaa
     2041 gaagctgata acttaaatcc caataaatta gaactaggta aggctttggg ttttgttgat
     2101 cgtttattaa tgtccttgtc ggctttatca ggttttgctt tagatgatat gacccaagac
     2161 aacagttggc gttttttaat gattggtcgg cgtttagaac gcttacagtt tttggcggat
     2221 atgattgctc aactattaca gcatgatgct gccaccgaac aaaccgcact cgactggtta
     2281 ttagagttag ccgatagcac catcacctat cgtagtcgtt atttgtccag tccacagctt
     2341 attcctgttt tggatttaat tttacttgac ccgagcaatc cacattcttt gcaatttcaa
     2401 ttagatagtt taagccaata tttacgtcag cttgatgata gtaatgatta tggcttatcg
     2461 cagatgagtg aacgtttaca gtccttaaat tttgcggtat tagaaagtga aatggattat
     2521 atccaacgtt tgccgcgtgc cttaatgttg attgcaaaac tattaaaatc gattgctgag
     2581 cagggacgac aactctccga tcaactcaat ttgcgttatt ttgctcatat cgaaaccttt
     2641 agccaaccca cggtgtctgc ctaatgaaaa cagcacgtta tcagatcacc cacgaaaccc
     2701 attatcgtta tgctgcacct gtttctttat cgcaacagtt attacatttg caggcgcgag
     2761 caatgccata tcaagcttgt ttacaacaag aattaaatat tcagcccgaa gcaagtgaca
     2821 gccaagcctt agaagatagt tttggcaatc cttatacccg tttagccttt aatcaaccac
     2881 atgatgaatt gttagttgtt tcaacaatga ccatagaagt attaccacgg caattagctg
     2941 attttaaaaa cagtatgccg tggcaaaaag tcgtggatgt gtgtcgttat caggggcaac
     3001 aaacttattc aagtgaatta gtcaacgcct tgccatatcg ttttcaatca ccgtatattc
     3061 ccttaaaaca tcaatttgcc gattatgcgg ctagctgttt tgacaaagac aaacctttgt
     3121 tattggctgt acaagattta atgcacaaaa tttttaaaga atttaccttt gaccctaaag
     3181 ccactcatat tggtacacca ttgttagaag tcttagaaaa aaagcgtggt gtttgccaag
     3241 actatgccca ttttatgatt gcctgtttac gctctttggg tttacccaca cgttacgtta
     3301 gtggctattt gttaacccat ccccccgaag gtcagccacg attgattggt gcggatgcgt
     3361 ctcatgcgtg gttagcggta tattgcccca tattaggctg ggttgacttt gatccaacca
     3421 ataatttaat tccaagcacc gaacatatta ccatggcatg gggaagggat tttagcgatg
     3481 tgtcaccact taggggcgtt attttaggtg gcggtagcca tgacttagat gtaagagtga
     3541 cggtaatgcc tattaccgag tagtgtttaa tttgatgtgt ttttaagttc ttgataagtt
     3601 aaaaataagc gtaaatcaaa ttctaattga tgataattag gctcgatatg ttgacaaagt
     3661 tgatagaaag ctttgttatg ggttggttct ttgaggtgag ccaattcatg tgcaacaatc
     3721 atcgttaaaa aatcagcagg tgtttgttta aataatgaag caatacgaat ttctttttta
     3781 gcttttaaac gactaccttg aactctagat accgctgtgt gtaaacctaa agcatgatga
     3841 accacatcta atttgttgtc ataacaaact ttatcaagat taggtgcatt ttttaaacaa
     3901 ttttgtttaa tattaaccgt aaattcgtat aaagatttat cagtttgaat ttggtgtgtg
     3961 ctaattggat agcgagtatt taaataggtt tctagtttgc cctgagcaat aagtagctgc
     4021 acttcgtttt gtaacgttgg tgaataggct cgtaaaaact ttaaatcgac cattggtgct
     4081 tgtctcagat attgtattta ttatgatatt tagagactag acgaaaaaaa aggtcttctc
     4141 aatgcttaaa tgcacgaaga aaaccttttt cgcttattaa ttggtcattc aaatacgacg
     4201 gcaacgccaa aagctaatta ttaatatacc aaaacaaaaa attaaaattg gccaattacc
     4261 taaacggcta aaaggtgttt gtcccttaac aggataaaca tggcctctta ataccgtacg
     4321 tttaaattgt ggggcttttt taacaagagt gcctttagga ctaataaacc cagaaatacc
     4381 cgtgttagtt gctctaataa tccatttgcc tgtttctaaa gcacgcattt gtaccatttg
     4441 caaatgttgg tgagggcctg tggatgtacc aaaccagcca tcgttgctta aagtagtaat
     4501 gaaatccgct ttttcggcat tacggcgagt aatttcgggg taagcaattt cataacaaat
     4561 agcagcagct aatttatggt ctttcacgat tagcgaggat tgttgtgggt taccagcact
     4621 aaaactcgac atttctaaat taaaaaaagg caaaacacca cgtaaccatg attcaaaagg
     4681 gacatattcg ccaaaaggca ctagacgttg tttaaaatat aaaccattgc ctgcgcctaa
     4741 agcggcgatg ctgttataaa aaggcggaat tccttgtttg ttgatattct ctaaatcggc
     4801 atagggaata cccgttacaa aagcagtatt agtagcggtg gcggtattgt tgattttaat
     4861 caaaaagtca ttagcttcat attgaaacat cggaatagcc gcttctggcc aaataacaac
     4921 atcttgtccc cattcacttt tggaaagctg ttcgtatatg gcgagtgttt tgtcgcgcca
     4981 ttccatttgc catttaacat cttgaggaat attaccttgg acaatactaa cggatagacc
     5041 ttgttgataa tcagattttg tccaagtgac gtattgtagg ccgtatccta cgccccaaat
     5101 aaggctaatc agtgcggcat aagaccatgc tttttgctga tatttagcaa tcattaataa
     5161 acattgtgca gaaacaaccg caaataaacc aacaccaaat acgcctaaaa tgggtgcata
     5221 agcgactaat actgaatcaa taaacgcata tccagtaaat agccaaggga agcctgtaaa
     5281 taaccacgtt tttaaccatt caaaaataat aaataacggt gcaaaagtta gcaaagcatg
     5341 tttgtcgagg ttaaaacgtc gataaagata agccattaag cctgtaaaag aacccatcac
     5401 gatggcaaca aatagcacca ttaaactggc tagccatgcg ggggtataac catattcatg
     5461 aatagaaaca aataaccaag aaacgccaac accccataaa cctgtaccaa aactccaacc
     5521 acgtaataag gcttgtttgg gtgtttgttg tggtaataaa tcggcaaata aggcaataca
     5581 cactaaagct aatggccaac aatgataagg tgctaagcta aaaggcaata aagcacccaa
     5641 aatacctgca taaattggcg caaatttgga gtgacgtaat ttggataaaa ggctcttcat
     5701 tatgtaatct tgcgtaagtc ttgcacacga atcacgctaa tcatgcgtaa atcagccgct
     5761 aagatagtaa atcgccaatg cactaaatca atatattcgt taatttgtgg catacgcgca
     5821 aattcactca aaataatacc accaatcgtt tcaaagtctt cgtggctaaa ctgactacca
     5881 aaataatcat taaattcggt gatgggggta atcgcttgta cgacatacgt cccgtctgct
     5941 tgttcactaa tccaatggga gccttcatcg gcatcatcat gttcatcgtg aatatcacca
     6001 acaatttgct ctaaggcatc ctctagggtc actagacctg caattccacc atattcgtca
     6061 acaacaatgg ccatgtgggt ttgtttttct ttaaattcgc gtaagaggcg atctaagtga
     6121 gcagattcgg gaacacaaaa agcagggcgt aaataatctt ttaaatcaaa acgatcagat
     6181 ttttgaccaa ttaaacgtaa taagtctttg gctaataaaa tacccacaac ttcgtctgaa
     6241 ttatcgctag ctaaaacggg aaagcgtgaa tgagcggatt cgataagaat aggtaaaaaa
     6301 tcttttggtt cgctatcatc acgaatcgac atgatttgcg ctctaggtac cataatttca
     6361 cgtacctgca tgtccgcaac gcgtaaagtt gattcaataa tttgttgaga ttcagcatct
     6421 aaaacgccac gtttgccagc actgtgaatg attgcaagca gttcttctcg gttttctggt
     6481 tcagtagata ataaattgct taatcggtta aaccaagatt taggcgtatc attgccacgc
     6541 tcatcgctca tatataatcc tattgggttc tatttttcgc gctgattata accttagctt
     6601 ttgtgaatct ccatgtattt aaatttttag aaaatgtgct aagttaagac cctaagcagt
     6661 tttagagatt aaaaatgaat aaattaatga cggtgttggt ttgtgtgttg accttagttg
     6721 cttgtcaaaa acaagagcct gttaaaacaa cagaagatac tggccgacca gaaacgcgca
     6781 gtattgaagc agccgatgct atggggtaca acggcaaaga ggttcgtaaa aaagttgatg
     6841 gagcgttaga tgccaatgat gcacataacg ctcaattaga acaacagaca aatgagcagt
     6901 aagttaagat ttcagtttag ttaagagttg ctcaacaaaa cttaaacgct tttggctgtc
     6961 agttaaatca ctgataaaac gtagcttttc tgctccatct aatttaaatt gagcaggttg
     7021 tttttgcacc aattgaacaa tagtgattgg ttgaaccgtt gtatctttag caaactccaa
     7081 acgaccacct tgagcatgag catcaatctt tttaatgcct aaatctgccg cttgtaattt
     7141 aattttgtgg gtggcgaata ggtttttagt ggcttcgggt aacaagccaa aacgatcaat
     7201 catttcgact tgtaattcgt ctaaagcttc gtaactctca gcatggctaa tacgtttata
     7261 catcaataaa cgattatgca cgtctggcaa ataatcatca ggaattaatg ccgataaacg
     7321 taaattaatt tctgcggtta aacttaatgg ttgttcgagg ttcggggttt tgccggcttt
     7381 aatggctttt acggctcttt ctaacatttc catgtaaagg gtaaagccaa cggcttgcat
     7441 attaccactt tgttcttcac ccaataattc acccgcacca cgaatttcta aatcttggct
     7501 ggctaacgca aaacctgcac ctaagtcatc agcattagca atggcttcga gtcgtttttc
     7561 ggcatcggca ctgagtaact ttttattggg aatcaataaa taggcataag cttgatgatg
     7621 ggaacgacca acacgaccac gtaattggtg gagttgtgct aaaccaagtt tatcagcacg
     7681 ctccataata atggtattgg ccgaaggcac gtcaatccct gtttcaataa tagtactaca
     7741 cactaaaaca ttaaattgct tgtggtagaa ctgttgcatg acatgctcca aatcacgttc
     7801 acgcatttga ccatgagcaa taccgacacg cgcttctggc aataaacctt gtaaatcagc
     7861 ggcacatttt tcaatcgttg ccacatcgtt atgtaaaaaa tacacttggc caccgcgtaa
     7921 taattcacgc agaatggctt ctttaatcac attgttatgg ctttcccgaa caaaggtttt
     7981 taccgccaaa cgtttggcag gaggtgtggc aataatcgat aaatcgcgta agcctgataa
     8041 ggccatattt aaggtgcgcg gaatgggggt ggcggtgagg gttaataaat ccacatcggc
     8101 acggcgattt tttaaggctt ctttgtggcg tacaccaaaa cgatgttctt catcgacaat
     8161 catcaagccc aagtctttaa actcaacact atcttgcaaa agtttgtgag tgccaatgat
     8221 gatgtcaatt ttgcctgtgg cgagttcgtc taaggctttt tgttgttctt tggcggtttt
     8281 aaagcgcgat aacacttcaa ttttaacagg ccagtcagca aaacgatcac aaaagttttc
     8341 atagtgttgt tgggcaagta gggtggttgg tactaaaacc gcgacttgtt tgccatcgtg
     8401 aattgagaca aaggcagcac gcatcgccac ttcggtttta ccaaagccca catcaccaca
     8461 caccaatcta tccattgggc gtggtgagcg taaatcggca atggttgata aaatggcatt
     8521 ggcttggtca gcggtttctt caaaggcaaa accagcacaa aacttggcgt attcttgctc
     8581 atcaaattta aaggcataac ccgctttggc ggcacgtctt gcactaatac ttaacaattc
     8641 ggcagccaca tcatgcactt gttcggcagc ttttttacgg gctttttgcc attgttcgtt
     8701 gcctagttta tgtaaggggg caagcgcatc ttctgaccct gaataacgtg caattaaatg
     8761 cagattggca acaggtacat acaatttggc attgtcggca taaattaact gtaaaaattc
     8821 ttgggtttgg ccatccatgt ctaaggtaat taagccttgg taacgtccca caccataatc
     8881 aatatgcacc acaggcacac caatggttaa ttcgcttaaa ctacgaatgg ctaattcggc
     8941 tgcaccttca cctgtggtgg ctttgcgacg gcgtttttgc ataacccgtt gaccaaacaa
     9001 ttggctttcg gcaatcacgg tcacatcagg caatgataga ccttgttcta agggtgcaat
     9061 ggttaaggca aaacgtagat ctttttcgat aaattcttgc caactattaa ccaatattgg
     9121 ttggaggttg gctttgttga gcaacgataa caggctttcg cggcgacctg cactttcggc
     9181 acacaacaaa attcgttgtg gttgctgctg aatataactg gctaaatggg cgagtggtgt
     9241 atcactacgc gagtctatgg gtaaattgat gggctgacta aaatcaaata agctagtgcc
     9301 tgctttttcg gcttcgtttt gttgatttaa ctgtaatctg gcaaagttat tcagttggcc
     9361 aaataactca ttactgcgta aaaataagct gtcaggcgat aaaatcggta ggtgattgtc
     9421 atggcgacga tcttcatatc gctgattaag ctctgtccaa aaatggtcta attgtgaatg
     9481 acagtcgctc atggtggcca ctaagctatg acgtggcaaa taatcacata aacttgcgtg
     9541 ctctttaaaa aagagtggta aataatattc aatacctgct gcggctaaac cttgattaat
     9601 gtcttgatat aacgcacatt ttttgggatc agtttgggga aatgtttctg cccactgatc
     9661 tttaaacaaa cgaatggcag tgcggtcaaa cggaaactct cgtgctggta ataattctaa
     9721 ttgttcaatt gtttcagtag tacgttgatt gtcaggatta aacaggcgaa gactgtcaat
     9781 ttcatcatca aataattcga tgcggacagg tgctgttgct cccatcggat aaatatccat
     9841 caagctacca cgcaccgcat attcaccgtg ttcataaacg gtttcaaccg cacgataacc
     9901 cacagcctct aaacgttggc gagtggcatc taaatcaaat ttttgaccgc attttaaact
     9961 aaagctttgt gccgataacc aactgggtgg tgctaaacgc tgagctaagg tagtaatggg
    10021 cgtaatcact aaacctttag tcaatgtcgg taacgtggct aaaatacgca aacgttcaga
    10081 aataatatct tgatgcggtg ataataaatc gtaaggtaag gtttcccaat cgggaaaatg
    10141 caaaataggg atttgctcgc cactaaaaaa acgaacttct tcttctaatc gtgccgcttg
    10201 ttgactatcg gcagtaatta ataacaccac gcctgaccat tgttgaatgg ctaaactcag
    10261 gcttaaacct gcgcctgagc ctaataaatt agcccatatt tttttgtcgc cagtatgcgt
    10321 aggaagaagc agttttgtgg ttaatgtcga gacagcagtc atagttggcc ttataagctc
    10381 atcatatttt aacgcgcgca ttgtagccga ttta
//
LOCUS       DAOQMB010000038        10398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2476_length_10398_cov_15.056850, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000038 DAOQMB010000000
VERSION     DAOQMB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2476_length_10398_cov_15.056850"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLY05_02685"
     CDS             <1..609
                     /locus_tag="PLY05_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02238.1"
                     /translation="VTKVATAPFRFLAGLVNGQDDIDNIPFDLANSQLNEQASTNVQK
                     VADILQKKADLQIEVRGNFNQQQELAALQQAKFKQLWNSNYPNQAFTISLLEKLYSQQ
                     IGAEDLAQQKVLTLKPSAEGQSLTTQTAIYQQTLIDKLVKAQPITEGDLRQLALERAK
                     SIRNQLVEKYQIAANRIFILEPNALELSQNQQVITNLTLKQD"
     gene            743..>1236
                     /locus_tag="PLY05_02690"
     CDS             743..>1236
                     /locus_tag="PLY05_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC02239.1"
                     /translation="MAFKAAESLAEQIARHVGRQIVTGDLVEGERIQELRIANELNVS
                     RGSVREALLILERRHLIEIYPRRGAVVSEMSAQQIRSLFEMIALLLGLIARRAADNWR
                     QHDVEPFTSLLDKLQNVARQGDIEAFYEGSFEFFHLSCRFAMNSYIEEMLEDLQPALQ
                     RSYY"
     assembly_gap    1237..1336
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1536..5012
                     /gene="smc"
                     /locus_tag="PLY05_02695"
     CDS             1536..5012
                     /gene="smc"
                     /locus_tag="PLY05_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HNC02240.1"
                     /translation="MRLKSIKLAGFKSFVDATTVPFPTNLSAIVGPNGCGKSNIIDAV
                     RWVMGESSARYLRGESMADVIFNGSSGRKPVGQASIELIFDNSDGTLGGEYARYNEIA
                     VRRQVNRDGKSDYFLNSSRCRRKDITDIFLGTGLGPRSYAIIEQGMISRLIEARPEEL
                     RVYIEEAAGISRYKERRKETETRLTNTRDNLARLNDIRDELTRQLSHLQKQADAAEKY
                     RALKTEERQTKIELFAWRWQTIQQELAQYELQIQQQAEQLAQSLDQQQHLEAALVLER
                     QKEEQAHQDLHGAQNHVYGVANSINQNQQQIAHLQQRRQQLLQQQQSVVLRQQQAQQE
                     SVQDAEEWAMLDAQLNELKPVLDELHALLAEQNSQLFDAEQNLLQQQQAYEIKQQQIA
                     QSTSQVQVWQAKVQAYEQALLRSQERQQRLQKELAEFATDGLLDDVAMQQEALAELDV
                     LLQDEQAELEQLSDKLTQAAQQSQQLQQNLHKLKSEQQKAEGQQSALQALQQAAVGRD
                     NQQQYRWLQQQGLAEQPRLAEVLKVEQGFEPLVETVLADVLKAIVVDDISQIDVSQSP
                     ALVLIERGANTALKQGSLAQNLVSEIAQDLLTQVYAVADLPTALAQRQQLANGESIIT
                     EDGYCVGKTWLKVLGKGEDSVLARQHSLEALQQQLAHLHQEISEAQQQQVQLNQLQQQ
                     MEQQRLQAQRQLSSHLQQRGAEQAKLAKLQAQLTQTQLQAQRLRQELEELAEQRELDQ
                     ESLAESRMQLEQHLQDLQEQQLLIEDLQQARENNRVLLNNARFSVQQTQQQNHQTALK
                     VQGLETRLAALSQNKQRQQHYLMSLDQQTVDMQRDIEALIEPVAELKLVLEELMAEKL
                     IAEQTLQQMEQDLHSVTQYYRQLEQQRQTLVMSLEPLRQQLEKNRLQWQNLSAHQGHI
                     VEQLKEFNINTEIIANYLNVQINETDWQMRLEQLANKISRLGAINLAAIDEYQQQAER
                     KQYLDNQAQDLEEALTKLEAAIKHIDKETRSLFIDTYNRVNEGLQHLFPKVFGGGMAS
                     LSLVGEDTLTSGVSIMARPPGKKNATIHLLSGGEKALTALALVFSIFQLNPAPFCLLD
                     EVDAPLDDANVGRFCRLVEEMAQQVQFIYITHNKIAMSMATQLMGVTMHEPGVSRLVT
                     VNVEQAAALAAA"
     gene            5023..5886
                     /locus_tag="PLY05_02700"
     CDS             5023..5886
                     /locus_tag="PLY05_02700"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HNC02241.1"
                     /translation="MTIEMGIGIALVVALVVLAKTIFDLSQQKKQQARIEPALTDECT
                     IVDGFPIIARASRTHLPKVGNIEPVLAAEQSADFTQTFAANEELDTVKFVVEQPVVQQ
                     SNVAILPVRHEPRIEPVAEEDNVADDEFIDTVAMSQSQSKVVEGQDYVVLYVTSGRVP
                     FDGERLLKAVSNYGLRFGEMGMFHRHEHPSGHGQVLFSMARVDESGAFDLEAMGSEFI
                     NAVTLFVALPNQQPLLAYDMMIDTAKRLAHEFCGQVLDQNQQPLNRQLTEHYREFVME
                     FTRHHLMSKAV"
     gene            6052..8106
                     /gene="ligA"
                     /locus_tag="PLY05_02705"
     CDS             6052..8106
                     /gene="ligA"
                     /locus_tag="PLY05_02705"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNC02242.1"
                     /translation="MSNFEFNFGNENVLSEQIIQQALYLREQLRHHNHCYYVLDNPQI
                     SDAQYDILFRQLLQLEKDYPQLQSSDSPTQKVGGEALSAFNSIEHRIPMLSLGNVFNQ
                     DELNDFERKIKERLETSRDIEYCAELKLDGLAISLIYENGLFKQGATRGDGTTGEDIS
                     HNLKTIRNLPLQLNTLTPPAILEVRGEVLMPKAGFHKLNAEAQQKGEKIFANPRNAAA
                     GSVRQLDPKIAAQRPLAFYAYAVAQIEGQELPKTQYEVLQWLKSLGFTISDEIQTGIG
                     ANFAAQFFAKIQQQRSQLPYDIDGVVIKVNELAKQQRLGIVSREPRWATAYKFPAELA
                     STMLEAVDFQVGRTGALTPVARVKPVFVGGVTISNITLHNMDEIKRLDLAIGDTIEVC
                     RAGDVIPKVTQVLHKADQREVIVLPHQCPICQSAIVQDEKGVIARCSGDFYCEAQVQR
                     RLAHFVSRKAMNMDGLGERWLEQFLEAGLITNIADLYALTKEKILAENLEGMGERLAE
                     KIVNAIEQSKTTTLPRFIYALGIRGVGESTALALAQHFASLEAIQQATEETLKQVPDI
                     GEVSAKWIVAYFQEPIHQQMIAKMQNAGVSWPAMEQRGEQPLTGQIWVLTGSLTTMAR
                     DEAKAKLQQLGAKVSGSVSKKTTVVVAGAEAGSKLADAQKLGVTVWDEQQLIALLQEH
                     QG"
     gene            complement(8103..8768)
                     /locus_tag="PLY05_02710"
     CDS             complement(8103..8768)
                     /locus_tag="PLY05_02710"
                     /inference="COORDINATES: protein motif:HMM:NF018604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HNC02243.1"
                     /translation="MTQKLPAWIEIGAFILALIAGLINVVGLLGFQHQSVSHLTGTVS
                     LFSLKIVQGEWLAVQHFMGVMLSFFMGAMLSGLIIQNATLKLGRRYSVALFIESLLLI
                     FALIMLQGNYSAGDFWASAACGLQNAMISTYSGAVIRTTHVTGTVTDLGALIGQYLRG
                     HSIDKRKIGLYLILLGGFIAGGLIGAYGFLHYSYYVLLMPILATQTLAVLYAWYWYQQ
                     QKT"
     gene            8852..>9129
                     /locus_tag="PLY05_02715"
     CDS             8852..>9129
                     /locus_tag="PLY05_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02244.1"
                     /translation="MFESAEIGHKIDKSVYKIEEPLLREALLDAQYELKEHKDFPVVI
                     LINGVDGAGKGETVNLLNEWMDPRLIATCAFGQPSDEEEERPRMWRYWR"
     assembly_gap    9130..9229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9230..10156
                     /locus_tag="PLY05_02720"
     CDS             <9230..10156
                     /locus_tag="PLY05_02720"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate--AMP phosphotransferase"
                     /protein_id="HNC02245.1"
                     /translation="YDKFKEVSESVLRETSTAEAPWIVVEGEDANYRSLTVGKLILQE
                     IKQRLAQQPKVMTQSDAAPLLPPIDNLQLLDTLDLDKSYSKKDYQEDLEKWQGKLNLL
                     SRHPKFKEHSVIAVFEGNDAAGKGGSIRRITAALDARQYTIIPIAAPSEDERVQPYLW
                     RFWRHIPRKGRFSIFDRSWYGRVLVERVEGYCQEHEWLRAYSEINDFEQQLVEHGAIV
                     VKFWLAISKEEQLKRFQEREQIGFKRFKITEEDWRNRDKWDNYVHAVGDMVERTSTDI
                     APWTLVEANNKYYARIKVLKTLCEAIEKALDV"
BASE COUNT         3345 a         1799 c         2200 g         2854 t
ORIGIN      
        1 gtgaccaaag tagcaaccgc tccgtttagg tttttagcag gtttggtcaa tggccaagat
       61 gatattgata atattccctt tgacctagct aatagccagc ttaatgaaca agccagtacc
      121 aatgtgcaaa aagtagccga tattttgcaa aagaaagctg atttacaaat tgaagtacga
      181 ggtaatttta atcaacaaca agagttggca gccttacaac aagcaaaatt taagcaatta
      241 tggaatagca attatcctaa tcaagccttc actattagct tattagaaaa gctctatagt
      301 caacaaatag gtgcagaaga cttggctcaa caaaaagtat taacactaaa accgagcgca
      361 gagggacaga gtttgaccac acaaacggca atttatcaac aaacactgat cgataaattg
      421 gttaaagcac agcctattac tgaaggtgat ttaagacaat tggctttaga gcgggcaaag
      481 agcattagaa atcaattagt tgaaaaatat caaatagccg ctaatcggat ttttatctta
      541 gaacctaatg cccttgaatt atctcaaaat caacaggtta ttactaattt gaccttaaaa
      601 caggattaaa atatgtaaga ttgtttgaca aaagttgttg agactgtata gtggctcatg
      661 ctagcatctt tttgtgaatg ggtacaaata gaacaaggtc attttatcga ctgttcttat
      721 taggctatac agggtgtgaa tcatggcttt taaagcggca gagagtttag cagaacaaat
      781 tgctcgacat gtgggacgac aaattgttac aggtgactta gttgaaggtg agcgtattca
      841 agagttgcgt atcgccaacg agcttaatgt cagtcgtggc tctgttcgtg aagccttatt
      901 gattttggaa cgtcgtcatt taatcgaaat ttatccgcgt cgtggtgcgg tagtctcgga
      961 aatgtctgcc caacaaatcc gtagtttatt tgaaatgatt gctttgttat taggcttaat
     1021 tgcgcgtcgc gcggctgata attggcgtca acacgatgta gagccattta catctttatt
     1081 agataaactc caaaatgttg ctcgtcaggg cgatattgaa gcattttatg aaggttcatt
     1141 tgagtttttt catttaagtt gtcgttttgc gatgaatagt tatattgagg aaatgttgga
     1201 agaccttcag ccagcattac aacgtagtta ttatttnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnaata ttttgttgca aattcccgat aagctgtcta tttttttcga
     1381 tagaatttgt acgattttta ctacaaggac gccgtaaagc gctgttttca gacttaaaag
     1441 tttgttaatc aaaatttttg atagcttcaa tcaaaaatca gctcattcgt ttatggatga
     1501 tttatctttt acccctaatt aatgaataaa taaccatgcg ccttaaaagt attaagctag
     1561 cgggttttaa atcatttgtg gatgccacca ctgtgccatt ccctacgaat ttatctgcca
     1621 tcgtagggcc aaatggttgt ggtaaatcca atattatcga tgccgttcgt tgggtaatgg
     1681 gtgagtcgtc tgcgcgttat ttacgtggcg agtcaatggc ggatgttatt tttaatggtt
     1741 ctagtggccg taaacctgtt ggccaagcca gtattgaatt aatttttgat aatagtgatg
     1801 gcacattagg cggagaatat gcccgttata atgaaattgc ggtacgtcgc caagtcaatc
     1861 gtgatggtaa atctgattat tttttaaata gcagtcgttg tcgtcgtaaa gatattactg
     1921 atattttttt gggaacagga ttaggccctc gctcttatgc aattattgaa caggggatga
     1981 tttcacggtt aattgaagca cgccctgaag aattacgggt ttatattgaa gaagccgcag
     2041 gtatttctcg ttataaagag cgtcgcaaag aaacagaaac ccgtttaact aatacgcgtg
     2101 ataatttagc gcgtttaaat gatattcgtg atgaattaac ccgtcagtta agccatctgc
     2161 aaaagcaagc ggatgctgcc gaaaaatatc gcgccttaaa aaccgaagaa cgtcaaacta
     2221 aaatcgaatt atttgcatgg cgttggcaga ctattcagca agagcttgcc caatatgaat
     2281 tgcaaattca acagcaagcc gaacagttag cgcagtcatt agatcagcaa cagcatttag
     2341 aagcagcgtt ggtgcttgag cgtcaaaaag aagaacaagc tcatcaagat ttacatggtg
     2401 ctcaaaatca tgtttatggt gtagcgaata gcatcaatca aaaccaacaa caaattgccc
     2461 atttgcaaca gcgtcgtcag caattattgc aacaacaaca gagcgtggtt ttacgtcagc
     2521 aacaagcaca acaagagtct gtgcaagatg ctgaagagtg ggcaatgttg gatgctcaac
     2581 tcaatgaatt gaagcctgtg cttgatgaat tgcatgcgtt attagccgaa caaaacagcc
     2641 aattatttga tgctgagcaa aacttattgc aacagcaaca agcttacgaa attaagcaac
     2701 aacaaattgc ccaaagcacc tcacaagtgc aagtatggca agctaaagta caagcttatg
     2761 agcaagcttt attgcgtagc caagaacgtc aacaacgctt gcaaaaagag ttagctgagt
     2821 ttgcgaccga tggtttatta gatgatgtgg cgatgcagca agaagctttg gctgagcttg
     2881 atgtgttatt acaagatgag caagccgagc ttgaacaatt aagcgacaag cttactcagg
     2941 cagcacaaca gtcacaacag ttacagcaaa acttacataa actcaaaagt gaacagcaaa
     3001 aagctgaagg ccaacaatcc gccttacaag ctttgcaaca agcagccgtg ggacgtgata
     3061 atcaacagca gtatcgctgg ttacaacaac aaggcttagc tgagcaacca cgtttggctg
     3121 aggtattaaa agtagaacag ggttttgagc ctttggtcga aactgtgtta gctgatgtat
     3181 taaaagcgat tgtggttgat gatattagcc aaattgatgt tagtcaaagt cccgcattag
     3241 tgttgattga gcgtggtgca aacacagcat taaaacaggg aagcctcgca caaaatcttg
     3301 tttcagagat tgcccaagat ttattaacac aggtttatgc cgttgccgat ttgccaacag
     3361 ccttggcaca acgtcaacaa ctggctaatg gtgagtctat tatcaccgaa gatggttatt
     3421 gtgttggtaa aacatggttg aaagtgttgg gtaaaggtga agatagtgtt ttagctcgcc
     3481 aacacagctt agaggcttta cagcagcaat tagcgcattt acaccaagag attagtgaag
     3541 cgcaacaaca gcaagtgcag ctcaatcaat tacaacaaca aatggaacag cagcgtttac
     3601 aagcacaacg ccaactgtcg agccatttac agcagcgtgg tgcggagcaa gctaaattag
     3661 ccaaattaca ggcacaatta actcaaactc agttacaagc ccagcgtttg cgccaagagt
     3721 tagaagagtt agctgaacag cgtgagttgg atcaagagtc attagctgaa tcacgcatgc
     3781 agttagagca gcatttacaa gatttgcaag aacagcaatt gttgattgag gatttacaac
     3841 aagctcgaga aaataaccga gtattattaa ataatgcgcg ttttagtgtg cagcaaaccc
     3901 aacaacaaaa tcatcaaacg gcattaaaag ttcaaggttt agaaactcgt ttagcagcat
     3961 taagtcaaaa taaacaacgt cagcaacatt atttaatgag tttagatcaa caaactgtgg
     4021 atatgcagcg tgatattgaa gcattaatag aacctgtggc tgaattaaaa ttagttctcg
     4081 aagaattaat ggcagaaaaa ttaattgctg aacaaacatt acagcaaatg gaacaagatt
     4141 tgcattcggt gactcaatat tatcgacagc ttgaacaaca acgccaaaca ttagtgatgt
     4201 ctttagagcc attacgccaa caattagaaa aaaatcgttt gcaatggcaa aatttatcgg
     4261 cacatcaagg tcatattgtc gagcagttaa aagaatttaa tattaatacc gagataatag
     4321 ccaattatct aaatgtacaa ataaatgaga cagattggca aatgcgcttg gagcaattag
     4381 ccaataaaat atcgcgatta ggtgctatta atttagccgc gattgatgaa tatcaacagc
     4441 aagcagaaag aaaacagtat ttagataatc aagcccaaga tttagaagaa gcattaacta
     4501 aattagaagc agcgattaaa catattgata aagaaacacg tagtttattt attgatactt
     4561 ataatcgggt aaatgaagga ttacagcatt tattccctaa agtatttggt ggcggtatgg
     4621 cgtcattaag tttagtggga gaggatacct taaccagtgg cgtgagcatt atggcgcgac
     4681 caccgggtaa aaagaatgca acaatccatt tattatcggg tggtgaaaaa gcattaacgg
     4741 ctttagccct agtattctcg atatttcaat taaatcctgc accattttgt ttattagatg
     4801 aagtggatgc accattagat gacgcgaatg taggacgttt ttgccgcctc gtggaagaaa
     4861 tggcgcaaca agtacaattt atttatatca cgcataataa aattgcgatg tcgatggcaa
     4921 cgcaattaat gggcgttact atgcacgaac ctggtgtttc acgtttggtg accgttaatg
     4981 tagaacaagc cgcagcctta gccgctgcct gagagatctg ttatgacgat tgaaatggga
     5041 atcgggatcg cccttgtggt tgctctcgtt gtgttggcca aaacaatatt tgatttgtcc
     5101 cagcagaaaa aacaacaagc tcgtattgaa cctgctttaa ctgatgaatg taccattgtt
     5161 gatggttttc cgattattgc acgtgcttca cgtacacatt tacctaaagt aggtaatatt
     5221 gagcctgtat tagctgcaga gcaatctgct gattttacac agacttttgc cgctaatgaa
     5281 gagttagaca ctgtgaagtt tgttgttgag caacctgttg ttcagcaaag caatgtggca
     5341 attttaccag ttcgccacga gccacgtatt gagcctgttg cagaagaaga caatgtggca
     5401 gatgatgagt tcattgatac cgttgcgatg tctcaatcac aaagcaaagt ggttgaaggt
     5461 caagactatg tggttttata tgtgacttct ggtcgtgtgc cttttgatgg cgagcgttta
     5521 ttaaaggccg tatcaaatta tggtttacgt tttggcgaaa tgggtatgtt ccatcgtcac
     5581 gaacatccaa gtggccatgg ccaagtatta tttagcatgg ctcgtgttga tgaatcaggt
     5641 gcatttgatt tagaagcaat gggttctgag tttattaatg ccgtgacctt gtttgttgcc
     5701 ttaccaaatc aacaaccttt attagcttat gatatgatga ttgacacggc taagcgttta
     5761 gctcatgaat tctgtggcca agtattagat caaaatcaac aaccattaaa ccgtcagtta
     5821 acggaacatt accgtgaatt tgtcatggag tttactcgtc accacttgat gagcaaagct
     5881 gtttgatctt ctatcgtcat aatagagcca tagacttatt gtaaataaat ataagtctag
     5941 aaaaacaaaa gccccatttt tggggctttg tttttgcgtc agataagtca taagtagtcg
     6001 tttaatagcg tagctagcta gtatattttt ttgtcttgag tcattatcag tatgagtaat
     6061 tttgaattta attttgggaa tgaaaacgtg ttatcggaac aaattattca gcaggcatta
     6121 tacttaagag agcaattacg tcatcataat cactgttatt atgtgttaga taatccacaa
     6181 attagtgatg cacaatacga tattttattt aggcaattac tacaattaga aaaagattat
     6241 ccgcaattac agagctcaga ttcacccaca caaaaagttg gcggtgaggc attatcagca
     6301 tttaatagta ttgagcatag aataccgatg ttatcgctgg gtaatgtttt taatcaagat
     6361 gagttaaatg attttgagcg aaaaataaaa gaacgtttag aaacgagtcg agatattgaa
     6421 tattgtgccg aattaaaatt agatggttta gcgattagtt taatttatga gaatggacta
     6481 tttaaacaag gtgcaacacg tggtgatggc acgacgggcg aagatattag tcataatcta
     6541 aaaaccattc gaaatttacc attacagtta aataccttaa caccaccagc aatattagaa
     6601 gtgcgtggtg aggtcttaat gcctaaagcg ggttttcata aattaaatgc tgaagctcaa
     6661 caaaaaggcg aaaaaatttt tgctaatcca cgcaatgcgg cggcaggcag tgttcgccag
     6721 ttagacccca aaattgcagc ccaacgacct ttggcatttt atgcctatgc tgttgcccaa
     6781 attgaggggc aagaattacc aaaaacccaa tatgaagttt tacagtggct gaaaagcttg
     6841 ggctttacca ttagcgatga aattcaaact ggcattgggg ctaattttgc cgcgcagttt
     6901 tttgccaaaa ttcagcagca acgtagccag ttaccttatg acattgatgg ggtggtgatt
     6961 aaagtcaatg agctagctaa acaacaacga ttaggcattg ttagtcgtga gccacgctgg
     7021 gcaactgcct ataaatttcc tgccgaatta gcgagtacca tgttagaggc cgttgatttt
     7081 caagtgggac gaacaggtgc attaacaccc gttgccagag ttaagccagt ttttgtcggt
     7141 ggtgtgacga ttagtaatat taccttgcat aatatggacg aaattaaacg tttagatttg
     7201 gcgattggtg acaccattga agtgtgtcgt gcaggggatg taattcctaa agtgacacag
     7261 gtgttacata aggcagatca aagagaggtg attgttcttc ctcatcaatg tcccatttgc
     7321 caatcagcca ttgtccaaga cgaaaaaggc gtgattgctc gttgttcggg cgatttttat
     7381 tgcgaagcac aagtacagcg gcgtttagca cattttgtct cacgtaaagc gatgaatatg
     7441 gatggtttgg gtgagcgttg gttagagcaa tttttagaag ctggtttaat tactaatatt
     7501 gctgatttgt atgcattaac gaaagaaaaa atcttagccg aaaaccttga aggtatggga
     7561 gagcgtttag ccgagaagat tgttaatgcc attgagcaaa gtaaaactac cacgttgcca
     7621 cgttttattt atgccttggg tattcgtggc gtgggagaaa gtaccgcttt agccttagca
     7681 caacattttg caagcttaga agccattcaa caagccacag aggaaacctt aaaacaagtc
     7741 cctgatattg gtgaggtctc ggcaaaatgg attgtggcgt attttcaaga gccaattcat
     7801 caacaaatga ttgccaaaat gcaaaatgca ggcgttagtt ggccagcgat ggaacaacgt
     7861 ggagagcaac ccttaacagg ccaaatttgg gtgctaacag gttcattaac tacgatggca
     7921 cgagatgaag ctaaagcaaa actacaacag ttaggtgcaa aagtcagtgg tagcgtctcc
     7981 aaaaaaacca ctgtggtggt tgcaggtgca gaagcaggtt ctaaattggc cgatgctcaa
     8041 aagttaggcg tgacggtttg ggatgagcaa cagttaatag ctttgttaca agaacatcaa
     8101 ggttaagttt tttgttgctg ataccaatac cacgcataca agacagccag agtttgtgtg
     8161 gctaaaatag gcatcagcaa cacataataa ctgtaatgta aaaagccata agcacctatt
     8221 agtccaccag caataaaccc gcccaataaa attaaataca agccaatctt gcgtttatca
     8281 atactatgac cacgcaaata ttgaccaatt aacgccccta aatcagtcac cgtacctgtc
     8341 acgtgagtgg ttctaatcac tgcaccacta taagtgctaa tcatggcatt ttgtaaacca
     8401 caagcagcag atgcccaaaa atcacctgca ctataattgc cttgcaacat aatcagcgca
     8461 aaaattaaca aaagactttc tataaatagc gcaacactat aacgtctgcc gagtttgagt
     8521 gtggcatttt gaataattaa accactgagc atagcaccca taaaaaaact cagcatcaca
     8581 cccataaaat gttgcacggc tagccattcg ccttgaacga tttttaaact aaataaagaa
     8641 acagtacccg ttaagtgtga tacggattga tgttgaaaac caagcaagcc aaccacatta
     8701 atcaagccag caattaacgc taaaataaaa gcaccaattt cgatccatgc aggaagtttt
     8761 tgagtcatag cgtaatttca cagataaact tgacattata atttgtaagg actaagacta
     8821 aataaattat aactgataga aggaaagata aatgtttgag tcagccgaaa ttggccacaa
     8881 aattgataaa tcggtttata aaattgaaga accattactc agagaagcct tattagatgc
     8941 acagtatgaa ttaaaagaac ataaagattt tcctgtggtg attttaatta atggtgttga
     9001 tggtgcaggc aagggagaaa ccgtgaattt gctcaatgaa tggatggatc cgcgtttaat
     9061 tgcgacttgt gcgtttggcc aaccttcgga tgaagaagaa gagcggccaa gaatgtggcg
     9121 ttattggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt acgataaatt
     9241 taaagaagtg tcggaatctg ttttgcgcga aaccagtact gccgaagccc cgtggattgt
     9301 ggttgagggt gaagatgcta attatcgcag tttgacggta ggcaaactta ttttacaaga
     9361 aattaaacag cgactagcac aacaacctaa ggtgatgact caaagtgatg ctgcaccctt
     9421 attaccgcca atcgacaatt tacaattatt agatacctta gacttagaca aaagttatag
     9481 caaaaaagat tatcaagaag atttggaaaa atggcaagga aaactaaatt tactcagccg
     9541 tcatccaaag tttaaagaac atagtgttat tgctgttttt gaaggtaatg atgccgcagg
     9601 caaaggaggt agtataagac gcattacagc cgccttagac gcgcgtcaat atactattat
     9661 ccctattgct gcacctagtg aagatgaacg tgtacagccc tatttgtggc gtttctggcg
     9721 acatattcct agaaaagggc gatttagtat ttttgatcgt tcatggtatg gccgtgtatt
     9781 agtcgaaagg gtagagggat attgtcaaga acatgaatgg ttacgcgcct atagtgaaat
     9841 taatgatttt gaacagcaat tggtcgaaca tggcgcgatt gtggtgaagt tttggttagc
     9901 gattagtaaa gaagaacaat taaaacgttt tcaagagcgc gaacaaatag gctttaaacg
     9961 ttttaaaatt accgaagaag attggcgcaa ccgagataaa tgggataact atgtgcatgc
    10021 tgtgggcgac atggttgagc gtaccagtac cgatatagca ccttggactc tagtcgaagc
    10081 aaataataaa tactatgcac ggatcaaagt gctaaaaact ctatgtgaag cgattgaaaa
    10141 agccttagat gtttaaaaaa atacacttta attgctaaaa acatgatcta aaaagaggtt
    10201 ttggctataa aataagctat taagcggttt tagataaaaa aggtttgaca gtgtttaata
    10261 acgctatata atgcgcgtcc tgagtcgggc atgactagct aaaaagatgt ttgaaatcaa
    10321 taagttaatg atttttaggt gtatttttag cggattttga agtgttgaaa ataagttgaa
    10381 aaaacttgtt gacaatac
//
LOCUS       DAOQMB010000039        10246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2535_length_10246_cov_14.611912, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000039 DAOQMB010000000
VERSION     DAOQMB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10246
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_10246_cov_14.611912"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="pdhA"
                     /locus_tag="PLY05_02725"
     CDS             <1..972
                     /gene="pdhA"
                     /locus_tag="PLY05_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HNC02246.1"
                     /translation="AYEHLWRTRLLDQRAISLQRNGQLGTYASCLGQEAISTAIGFSL
                     QPDDVFVPYYRDQAAQMLRGTKMRSILQFWGGDEWGSHDAPKQDLPACIPIATQITHA
                     AGVATALKLQHIKQATLTTCGDGASSRGDFYEALNLAGVWHLPLVVVINNNQWAISVP
                     LSQQTASETLAQKAIAAGVLGVRVDGNDFFALMAVLAEALERARSGKGATLIEAITYR
                     LCDHTTADDMSRYADANQRATAKEHEPLLRFRRLLDQQLNWTEAQQQQLEQKLQQEIE
                     QEIQAYLQLPSPTVNDMFDYLYEQLPTELQQQKQQFLTAKGGFHHAP"
     gene            962..1936
                     /locus_tag="PLY05_02730"
     CDS             962..1936
                     /locus_tag="PLY05_02730"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HNC02247.1"
                     /translation="MLLNLVEAVDLALARALSEDESVIILGEDVGKNGGVFRATVGLR
                     EKFGAKRVLDTPLAETLIAGVAVGMSTQGLRPVAEIQFMGFIYAALEHLISHAARLRN
                     RTRGRISCPLTLRVPFGGGIHAPEHHSESTEALLTHIAGLKVVALSSPRTAYGLLLAA
                     IRCNDPVIVLEPERIYRKFKEEVIDDGQQIDLEHCLIWRKGKDLSLVAWGAAVQDCLN
                     AAEQLAQQGIDAEVIDVACLKPLDMTTIINSVALTGRCIIVQEAVKSGGLGSDIAARL
                     ADKAFYALRAPIKIISPPDVIVPYFKLEQAYLPQVADIIQASRALMEA"
     gene            1940..2986
                     /locus_tag="PLY05_02735"
     CDS             1940..2986
                     /locus_tag="PLY05_02735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HNC02248.1"
                     /translation="MKNLLLADLGEGLTEATVVEYYVSPKQLVKQGDLLLAVETAKAI
                     VEIPAPETLIVQQLLATANQVVNVGMPLLSYDSPQAELNTTPTTHDTSSVVGQLEISG
                     EQYQQNFITGQQRFSEQERHTIQQRLLNKNNSPIIVERQHIPLSGSRLVMAQNLAQAH
                     QQVALVTIFDEVLIKKINNPLITMIQALCYACQQQPKLNAWFDTQQLIIHQDVDLGLA
                     IDTEYGLYVPVIKQANKLNKQQLQQAITQLKTAAHQHQLSNAQLQGATISLSNFGTIA
                     GRFATPMIVPPQVAILGVGQIQKRYVINAKAKIKEKVSIPLSLSFDHRALTGGEAARF
                     LAAIKDFLHNKKAA"
     assembly_gap    2996..3095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3096..4000)
                     /locus_tag="PLY05_02740"
     CDS             complement(<3096..4000)
                     /locus_tag="PLY05_02740"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HNC02249.1"
                     /translation="MLKLQFVDRRQPAFWLVDPRLTIGRDKTNNLVINDEGISVFHAE
                     LQQEDGKLFLRDTGSVNGTFLNGEPVTKRVEVKVGDVIRLHLVELQIIDPTKAPPEPP
                     AQPVKRELPKAAPLWQVKAMTGVISGRMFPIEGTNVVGRDPHCDIVVGGAHVSRRHAE
                     FSIRSGVLWVKDLGSSNGSFVNGKRHEEVALQNGDEVKFDAMIFKVVGPTDLPAHSDF
                     EEEAERTQFRPVVNVPAAKPAAPKQPSPVPVNNSYSPPPSSPVSESKPQEQPIVSTPT
                     PSAGTDKAGSMGMIVIVILMILILVG"
     gene            complement(4016..5974)
                     /gene="gspD"
                     /locus_tag="PLY05_02745"
     CDS             complement(4016..5974)
                     /gene="gspD"
                     /locus_tag="PLY05_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HNC02250.1"
                     /translation="MNVRQSFLALSLILACHSVAEAKTWKVNLKDAEISALVSEVSEI
                     TGKNFIVDPRVKGNITVISSKPMSSEAVYELFLGVLSVNGFAAVPSGNVIKLVPDVNA
                     KQNAVPFENKAKAKGEALVTRVIMLENTNANELVPVIRPLLPQFAHLAAINGANALVI
                     SDHANNIVEIENLIKSLDAGEGDELEIIALKETRVDEILTMLDNLTATSPNKEMRGAR
                     VRLIADTRGNRLLIKGDARARKRLREVVAQLDKPAADRLAGVRVVRLKHASAKQVAEV
                     MRNLVSNDGSKSTNSGNTGNNSGATAINAGGVSLIADESQNALVIKADPSLMRELETV
                     IQQLDTRRSQVLIQAAILEISGENADALGVQWAAGDPTKGVGLVNFSNAGASLSSLAA
                     VAMQSNPTSIPSINGASFAVGKSETNSNGDRSFYGGLLQAIKTMSNANLLSTPSIMTI
                     DNQEAKIVVGQNVPFITGQSTSSSSGTTNPFTTIERQDVGITLKVKPHIGEGDTVKLE
                     VEQEVSSVVPSVAGVNSADLITNKRSIKTTILADDGQTIVLGGLIQDDNSQSVNEVPL
                     LNKIPLLGHLFRAKSHSQTKRNLLVFLQPLILRDGQNAADLAAQQYNQTRILQLEVSE
                     KGKLTRLPEQINQIYQGATKSQAPKVNK"
     gene            complement(5976..6812)
                     /locus_tag="PLY05_02750"
     CDS             complement(5976..6812)
                     /locus_tag="PLY05_02750"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNC02251.1"
                     /translation="MNVPSLSVITENNRHIARAIMLLATLYFAWRMAGLLWLIAGQDQ
                     AALAKPTPVSSAAAPVNVDSSRLASFTIFKAPVVESVSQNANAPDTSLQLKLDGVFAS
                     SSQSQSSAIISEQGQGIGKLYRVGQQVVGGAVLSAVYVDRVLLQRNGQEEVLRFIKSN
                     LLGGDTPPVASNPVASQADKAHSLLSNAIQRLDSDASSYLNEMGLIASQQGYEITDNT
                     PSHIRRNLGLKAGDRVVRLNGQTLGNVQLDKNLLQQIQQTGRARIEIQRGGQHLTIEQ
                     SF"
     gene            complement(6968..7729)
                     /gene="gspN"
                     /locus_tag="PLY05_02755"
     CDS             complement(6968..7729)
                     /gene="gspN"
                     /locus_tag="PLY05_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HNC02252.1"
                     /translation="MLKKTTNQEDKEAKAYNWVLLATVLLVFALLAQMPARIIANFVP
                     KDIRSLCTAWGGTLWTGQVNTQLNNLQGQLRWSFQPLSLLTLKLAFNVELLTGSSQIP
                     ANVVLGLGGWQIKASQGQLSPIDLQPLLSSWQLPNNPIMISNLNVVKKGQNWQDSQGV
                     LTWQAGAIDYVLNGQRQHLNLPPVALKLSGQQQSLIMSLQDEAQTVNLATFTITGAMI
                     ETRLTHRLLTYAPNYRGVAEPDAIVVTASQPLNSL"
     gene            complement(7932..>8180)
                     /locus_tag="PLY05_02760"
     CDS             complement(7932..>8180)
                     /locus_tag="PLY05_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HNC02253.1"
                     /translation="QLKTARAQLEKVAADFGYTLEELLSGKSVETVEDAPVKPRKPVE
                     IRYRNPANEAETWTGRGKQPRWLAAAIASGKNLQDFAI"
     assembly_gap    8181..8280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8281..8828)
                     /gene="ssb"
                     /locus_tag="PLY05_02765"
     CDS             complement(<8281..8828)
                     /gene="ssb"
                     /locus_tag="PLY05_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016651355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC02254.1"
                     /translation="MRGVNKVILLGTVGRDPEVRSLGASGSTVTSISLATSEAWKDKN
                     TGQLTEQTEWHRVVFYGRLAEISGEYLRKGSKVYVEGSIHTRKWQDKTTGQDRYSTEI
                     KANVMQLLDGRGGNNQSGGDYNNDYSYGNNNGGSSYGGGSNSYGQDDYNNRGGGSYGN
                     RNQARQPAAPAPMANDLDDDLP"
     gene            complement(9043..>10246)
                     /locus_tag="PLY05_02770"
     CDS             complement(9043..>10246)
                     /locus_tag="PLY05_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC02255.1"
                     /translation="GIYGLTQAVLQIPVGLWADRINRKLLIIVGLILFALGGAVAAVA
                     DSIWGVIVGRAIQGAGAISAVVMALLADLTREEQRTKAMSTIGMSIGMSFAVAFILGP
                     MVAGHFGLSGLFWATSVLAILGLSLLHLVPQPTVIIKQHHDSYWQQFKQSLLHPELAR
                     LNAGIFILHLVMTASFVLLPHLLIEYAHLPASQHGWVYLPIVLGGFIAAVPAIIIAEK
                     RRKIKQVFVFGISLMVLSLLVLAREYQHYWGLLFGISLFFIAFNLLEALLPSLLSKIA
                     PVGTKATAMGIYSSSQFLGAFFGGICGGVLWAKHDITLAYICLAILSLMWVLLAVFMR
                     SPRFLHSMMLNIPSCNSQQASQLLSKLQAIIGVEEAVVLAQEGVIYLKIDKNKLNEKD
                     LQQILISC"
BASE COUNT         3063 a         2243 c         1803 g         2937 t
ORIGIN      
        1 gcttatgaac acttatggcg gacacgcttg cttgatcaac gcgcaattag tctacaacgt
       61 aatggccaat tgggaactta tgccagttgt ttggggcaag aggcgattag caccgcgatt
      121 ggttttagcc tacaaccaga tgatgttttt gtaccttatt accgtgacca agcggcacaa
      181 atgctgcgtg gcacaaaaat gcgttccatt ttacagtttt ggggcggcga tgaatgggga
      241 tcacatgatg caccaaaaca agacctgcca gcctgtattc caattgccac ccaaattact
      301 catgccgcag gcgtagccac tgccctaaaa ttacaacata ttaaacaggc tacgttaaca
      361 acctgtggcg atggtgcaag ttctcgtggc gatttttatg aagcactcaa tttagcaggt
      421 gtgtggcatt tacccttagt ggtggtgatt aacaacaatc aatgggcgat ttctgtgcct
      481 ttaagccaac aaaccgccag tgaaaccctt gcccaaaaag ccatagccgc aggtgtatta
      541 ggcgttcgtg tcgatggtaa tgattttttt gcgctgatgg cagtattggc agaagcgtta
      601 gagcgcgcac gttcgggtaa aggcgcaacg ttaatcgaag ccattaccta tcgcctatgt
      661 gaccatacca ccgctgatga tatgagtcgt tatgcggatg caaatcagcg agcgaccgct
      721 aaagaacatg agccattatt aagatttaga cgtttattag atcaacaatt aaattggaca
      781 gaggctcagc aacaacagct tgaacaaaaa ttacaacaag aaatagaaca agaaatccaa
      841 gcttatcttc agcttccgtc accgactgtg aacgatatgt ttgattattt atatgaacaa
      901 ctacccacag agctacaaca acaaaagcaa cagtttttaa ctgcaaaagg aggatttcat
      961 catgctcctt aatcttgtcg aagcggttga tttagcctta gcgcgcgccc tcagcgaaga
     1021 tgaaagcgtg attattttag gtgaagatgt tggcaaaaat ggtggcgttt ttcgagcaac
     1081 tgttggcctg cgtgaaaaat ttggtgctaa acgtgtgtta gatacgcctt tagccgaaac
     1141 actcattgct ggcgttgctg tagggatgag tacacaaggc ttacgtcctg ttgctgaaat
     1201 tcaatttatg ggctttattt atgcagcctt agaacattta atttctcatg ctgcacgttt
     1261 acgcaatcgc acacgaggtc gtattagttg tccactcaca cttcgtgtgc cttttggtgg
     1321 aggcattcat gcgccagaac atcactccga aagcacagaa gctctattaa cccatattgc
     1381 aggcttaaaa gtcgtggctc tgtcttcccc tcgtaccgct tatggtttgt tattggcagc
     1441 cattcgttgt aatgaccctg tgattgtttt agagccagaa cgtatttatc gcaaatttaa
     1501 agaagaagtg attgatgatg gtcagcaaat cgacttagaa cactgtttaa tttggcgtaa
     1561 aggcaaagac ctcagcctag tggcatgggg cgcagccgta caagattgct taaatgcggc
     1621 tgagcaattg gctcaacaag gcattgatgc cgaagtaatt gatgtcgctt gtttaaagcc
     1681 cttagatatg accacgatta tcaactcggt tgctttaact ggccgctgta ttattgtcca
     1741 agaagcggtg aaatcgggtg gcttaggcag tgacattgcg gctcgcttag cagataaagc
     1801 tttttatgct ttacgcgctc ccataaaaat tattagccca ccagatgtga ttgttcccta
     1861 tttcaaactc gaacaagctt atctgccaca agttgcagac attattcagg caagccgtgc
     1921 cttaatggag gcctaaacca tgaagaactt attattagct gatttgggtg aaggtttaac
     1981 cgaagccact gtggttgagt attatgtatc ccccaaacaa ctggtcaaac aaggtgattt
     2041 attattagcg gtcgaaaccg cgaaagcgat tgttgagatt cctgcgcccg aaacattaat
     2101 tgtgcaacaa ctgttggcaa cagccaatca agtggtcaac gtgggtatgc cattattgag
     2161 ttatgactct ccgcaagctg agctaaatac cacacctaca acacacgata cttctagtgt
     2221 ggttggccaa ctagaaatca gtggcgagca atatcaacaa aactttatca caggccaaca
     2281 acgtttttct gaacaagaac ggcacaccat ccaacaacgt ttattaaaca aaaacaattc
     2341 accaattatt gtagaacgcc aacatattcc acttagtggc tcacgtttag tcatggcgca
     2401 aaacttagcc caagctcatc aacaagtggc tttagtgacg atttttgatg aggtgttaat
     2461 caaaaaaatc aacaatccct taatcaccat gattcaggct ttatgttatg cctgccaaca
     2521 acagcccaaa ctgaatgctt ggtttgacac acaacaatta attattcatc aagatgtcga
     2581 tttaggctta gccattgata ctgaatatgg cttatatgta ccagtcatta aacaagccaa
     2641 caaactcaac aagcaacaat tacaacaagc gatcactcag cttaaaactg ctgcccatca
     2701 acatcaactc agcaatgcac aattacaagg agcgacaatt agcctatcta actttggcac
     2761 aatagcaggc cgctttgcca caccaatgat tgtaccacca caagtggcca ttttaggggt
     2821 aggccaaata caaaaacgtt atgtgattaa tgctaaagcg aaaatcaaag aaaaagtcag
     2881 tattccgctt tcacttagtt ttgaccatcg tgcattaaca ggaggagaag cagcacgatt
     2941 tttagctgct attaaagatt ttttgcacaa taaaaaagcg gcctaagccg cttttnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncccac taaaatcaag atcattaata
     3121 taacaatcac aatcatgccc atgctaccag ctttatctgt accagcactt ggtgttggtg
     3181 tggacacaat tggctgctct tgaggcttag actcagagac agggctgctt ggaggaggac
     3241 tataactatt attaacaggc acaggcgatg gctgttttgg tgctgctggt tttgcggctg
     3301 gcacattaac cactggacgg aactgcgtac gctccgcttc ttcctcaaaa tcagaatgcg
     3361 caggcaaatc agttggacca accactttaa aaatcatcgc atcaaactta acttcatcac
     3421 cattttgtaa tgcaacttct tcgtgacgct taccattcac aaatgatcca tttgacgagc
     3481 ctaagtcttt gacccataaa acgccagaac gaatcgaaaa ttcagcatga cgacgcgaga
     3541 catgtgcacc accaacaacg atgtcacaat gaggatcacg acctacaaca tttgttcctt
     3601 cgataggaaa catacgccca gaaatcacac ctgtcatggc ttttacttgc cataatggcg
     3661 cagctttggg taactcacgt ttaactggct gtgcaggagg ctctggtgga gctttggttg
     3721 ggtcaataat ttgcaattca actaaatgca aacgaataac atcacccact ttgacttcaa
     3781 cacgtttggt aacaggctca ccatttaaaa atgtgccgtt aacactgcct gtatcacgca
     3841 aaaataactt accatcttct tgttgaagtt cagcatgaaa aacactgata ccttcatcat
     3901 taatgacgag attgttggtt ttatcacggc caattgttaa acgaggatca accaaccaaa
     3961 atgcaggttg gcgacgatca acaaattgta acttcaacat gatagtttct cttccctatt
     4021 tgttaacttt cggagcttga ctcttggtcg ctccttgata gatttggtta atctgttctg
     4081 gtaagcgggt taatttaccc ttctcactca cttctaactg caaaatgcgc gtctgattat
     4141 actgctgtgc tgctaaatct gcagcatttt ggccgtcacg caaaattaat ggctgcaaga
     4201 acactaataa atttcgcttc gtttggctat ggctttttgc tctaaataag tgccccaaca
     4261 aaggaatttt attcaaaagt gggacttcgt tcacagattg agaattatca tcctgaatca
     4321 acccacccaa aacaattgtc tgtccatcat cagccaaaat tgtcgtttta atcgaacgtt
     4381 tattggtaat taagtctgca gagtttacac ctgccacact cggaacaaca gaggaaactt
     4441 cttgttcaac ctctaatttt acggtatcac cttcaccaat atgcggttta actttaagag
     4501 taatacccac atcttgacgt tcaatggtgg taaatgggtt agttgtaccc gaactagagc
     4561 ttgtactttg cccagtaata aacggtacat tctgaccaac cactattttt gcttcttgat
     4621 tatcaatggt cataatactg ggggttgata ataaattggc attactcatg gttttaatgg
     4681 cctgtaataa accaccataa aaacttctgt caccattact attggtttcg ctcttaccaa
     4741 ctgcaaaaga agcaccatta atagatggaa tagaggttgg gttactttgc attgccacag
     4801 cagccaaact actgagactc gctcctgcat tcgagaagtt aactaaacct acacctttag
     4861 taggatcacc cgctgcccat tgtacgccta aagcatcagc attctcaccc gaaatttcta
     4921 aaatagccgc ttgaattaaa acttgtgaac gacgagtatc taattgctga ataaccgttt
     4981 ctagttcacg cattaatgat ggatcagctt taatcactaa ggcattttga ctttcatcgg
     5041 ctatcaggct tacaccacct gcattaattg ctgttgcacc gctattgttg cctgtattac
     5101 cactatttgt agatttacta ccatcattgc tgaccaaatt acgcatgact tccgcaactt
     5161 gtttggcact ggcatgtttt aaacgtacca cgcgcacacc tgccaatcgg tcagccgcag
     5221 gtttatcgag ttgtgcaaca acttcacgta aacgtttacg cgcacgagca tcacctttaa
     5281 tcaataagcg attaccgcgt gtatctgcaa ttaaacgaac gcgcgcacca cgcatttctt
     5341 tatttggact tgttgctgtt agattatcta acatggttag aatttcatct actcgtgttt
     5401 cttttagagc aatgatttct aactcatctc cttcgcccgc atctaaggat ttaatcaaat
     5461 tttcaatttc gacaatatta ttagcatgat cagaaatcac taaagcattt gcaccattaa
     5521 ttgctgctaa atgagcaaat tgtggcaata gcggtctaat aacaggtact aattcattag
     5581 cattggtgtt ttcaagcata ataacgcgtg ttactaatgc ttcaccctta gctttcgctt
     5641 tattttcaaa aggaactgca ttttgtttgg cattaacatc tggtactaat ttaatcacat
     5701 tgcctgaagg cacagcggca aagccattaa cacttaatac ccccaaaaat aactcataaa
     5761 ccgcttcaga actcatcggt ttactcgaaa tcaccgtaat attacctttg acacgcgggt
     5821 caacaataaa atttttacct gtaatctcag agacttcaga cactaaagcc gaaatctcgg
     5881 catcttttaa attaaccttc catgtttttg cttctgccac actgtgacaa gccaagatta
     5941 atgaaagtgc taaaaaggat tgacgtacat tcatgttaga agctttgctc aatagttaaa
     6001 tgttgaccac cacgctgaat ttcgatacgt gcacgcccag tttgttgtat ttgttgcaat
     6061 agatttttat ccaattgcac atttcccaaa gtttgaccat tcaaacgcac cactctatcg
     6121 ccagctttta agcccaaatt acgacgaata tgacttggtg tattatctgt tatctcatat
     6181 ccttgttgac tcgcaattaa gcccatttca ttaagatatg aacttgcatc actatccaaa
     6241 cgctgaattg catttgataa taaactatgt gctttatcag cctgactcgc cacaggatta
     6301 ctagcaacag gtggagtatc gccacctaat aaattacttt taataaaccg taagacttct
     6361 tcttgaccat tacgctgtaa taatactcga tcgacataaa cagcggataa taccgcacca
     6421 ccaaccactt gctgacctac acggtataat ttaccaatgc cttgtccttg ttcgctaata
     6481 atcgcacttg actgtgattg agagctactt gcaaaaactc catccaattt aagctgtaac
     6541 gatgtgtctg gcgcattggc attttgactg actgactcaa caacgggtgc tttaaaaata
     6601 gtaaagcttg ctaaacggct actatccaca ttgacaggtg ctgccgcact gcttacaggc
     6661 gttggtttag ccaatgcagc ttgatcttga ccagcaatta accataacaa acctgccata
     6721 cgccaagcaa aatataatgt tgccaacaac ataatagcac gagcaatatg tcgattattt
     6781 tcggtaatta cggacaacga aggcacgttc acactgattc ctaaacttta aattatactt
     6841 acgttaatag tatgactttt ttgcgacgat aatattaaaa agcacttaat atttacgcga
     6901 aacgtaagcc ttttattaag agttaatgct attttaaaat caagatgcat aaactcatat
     6961 tattatttta taaactattt aagggttgag atgcagtgac tacaattgca tcaggctctg
     7021 caacacctct atagttcggc gcgtaggtta acaaacgatg tgttaaacga gtttcgatca
     7081 tcgcgccagt aatcgtaaat gttgctaaat taacggtctg cgcttcatct tgcaaggaca
     7141 ttattaaact ttgttgttga ccactgagtt ttaaagccac aggaggcaaa tttaagtgct
     7201 gacgttggcc atttaagaca taatcaatag cacctgcttg ccaagttaac accccttgcg
     7261 aatcttgcca attttgccct tttttcacaa catttaagtt gctaatcata ataggattgt
     7321 ttggcaattg ccaactgctt aataaaggtt gtaagtctat tggcgataat tgcccttgac
     7381 tagcctttat ttgccatccc cccaatccca acactacatt tgcaggaatt tgcgaactac
     7441 ctgttaataa ctcaacatta aaagctagct ttaatgttaa taatgataag ggctgaaaag
     7501 accaacgtaa ttgaccttgc aaattattta gttgcgtgtt gacttgccct gtccacaaag
     7561 ttccacccca cgccgtacat aagctacgga tatctttagg aacaaagtta gcaataatgc
     7621 gggcaggcat ttgtgcaagt aaagcaaata ccaataatac tgttgccaat aagacccaat
     7681 tataagcttt tgcttcttta tcttcttgat ttgttgtttt ttttaacata aagactaact
     7741 ttaacaagta ttagataggg gctagcagag tgtgcccaat tttttaccat tttgatgctg
     7801 atttatctct atttttcaca aaaatacaag ctttattttg cagagtgttg ttattttata
     7861 aaccttttat tgattagaac acaaaaaagc ccccatatct ggaggcttta ctaaccatta
     7921 taaatcagcg atcaaatggc aaaatcttgt aaatttttac cagatgcaat agcggctgcc
     7981 aaccaacgtg gctgtttgcc acgtcctgtc catgtttcgg cttcgttagc aggattacga
     8041 tagcgaattt caacaggttt acgaggtttt acaggtgcat cttcaacagt ctcaactgat
     8101 ttacctgata ataactcttc taaggtataa ccaaaatcgg cggccacttt ttctaattgt
     8161 gcgcgagctg tttttaattg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 aatggcaaat catcatctaa gtcattagcc atcggtgctg gtgctgctgg ttgacgagct
     8341 tggttgcggt tgccgtaaga accaccacca cgattattgt agtcatcttg accatagcta
     8401 ttactgccac cgccataact actaccacca ttattattac cataactata atcgttatta
     8461 taatccccac ctgattgatt attaccacca cgaccatcta ataattgcat cacattagct
     8521 ttaatttcgg tagaataacg atcttggcct gtggttttgt cttgccattt acgagtatga
     8581 atagaacctt caacgtaaac cttactgcct ttacgtaaat attcacctga aatctcagct
     8641 aatcgaccat aaaatacaac acggtgccat tctgtctgct ctgttaattg ccccgtattt
     8701 ttatctttcc atgcttcact ggtcgctaat gaaatgctag tgactgtact gccactcgcc
     8761 cctaaactac gaacttctgg atcacgaccc actgttccta ataaaataac tttgtttact
     8821 ccgcgcatga cgttttgcac cctctatggt attgctctac aaaaaaatac tgtttactca
     8881 ataagataac aatgtctaac tctgaacaaa cgactacttg tgtcgtttaa attatcttaa
     8941 taccaaccaa cactaccgat taatagcaat cgataaatct taaataaaga tttttgattt
     9001 tgcatcaaaa ttaaggctgt tggcaacctc tttcatcaaa aactaacagc ttattaatat
     9061 ctgttgtaag tctttctcat ttaacttgtt tttatctatt tttaaataga taaccccttc
     9121 ttgtgccaag actacagctt cttctacgcc aataatcgct tgtagttttg ataataattg
     9181 tgaagcctgt tgcgagttac aacttgggat attcagcatc atactatgta aaaatcgtgg
     9241 tgaacgcata aaaacagcta ataataccca cattagactt agaatcgcta aacatatata
     9301 agctaaagta atatcatgtt ttgcccataa aactccacca catatccccc caaaaaaagc
     9361 ccctaaaaat tggctacttg aataaatgcc cattgcagtg gcttttgtcc ctactggtgc
     9421 aattttactt aataatgaag gtaataaagc ctctaataaa ttaaaggcaa taaaaaataa
     9481 actaatacca aataataagc cccaataatg ttggtattct cgtgccaaaa ctaacaaact
     9541 taataccatt aaactaatac caaagacaaa aacttgtttt atctttcgac gtttttcggc
     9601 aataataatg gctggaacgg ctgcaataaa tccacccaac acaatcggca aatataccca
     9661 accatgttga ctggcaggca aatgagcata ctcgattaat aaatgcggca ataacacaaa
     9721 actggcggtc atcaccaaat gtaaaataaa aatacctgca tttaaacggg ctaattcagg
     9781 gtgaagcaaa ctttgtttaa actgttgcca ataagaatca tgatgctgtt ttataatcac
     9841 cgttggctgt ggcactaaat gtaataaact taagcctaat atcgctaata ctgatgttgc
     9901 ccaaaataaa ccgcttaaac caaaatgccc agccaccatt gggcctaaaa taaaagcaac
     9961 cgcaaaggac attccaatac tcatgccaat cgtggacatc gctttggttc gttgttcttc
    10021 tcgggttaaa tcggccaata atgccataac caccgccgaa atagcccctg caccttgaat
    10081 cgctcgacca acaatcacac cccaaattga atctgcgact gccgctaccg caccaccaag
    10141 cgcaaataat atcaagccaa caataattaa taatttacga tttattctat ctgcccataa
    10201 acctactgga atttgtaata ccgcttgcgt taaaccataa ataccg
//
LOCUS       DAOQMB010000040        10169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2566_length_10169_cov_16.003856, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000040 DAOQMB010000000
VERSION     DAOQMB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10169
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2566_length_10169_cov_16.003856"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="PLY05_02775"
     CDS             <1..129
                     /locus_tag="PLY05_02775"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008305534.1"
                     /note="catalyzes the formation of dUMP from dUTP; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNC02256.1"
                     /translation="IQVGERLAQYVLVPVIQADLTVVDEFVATERGAGGFGHSGRH"
     gene            326..2794
                     /locus_tag="PLY05_02780"
     CDS             326..2794
                     /locus_tag="PLY05_02780"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HNC02257.1"
                     /translation="MAMPFVIGVTVLLLVIIAVIGFIAISDQKRQQVEEAKQYEQRAV
                     DVVNNFLEQQDDFAKQLASQPSEIIVGKIAGAVLAQVVLTGDETTPKSLSFSNQDLLR
                     RAAAQGGNVTAEMSVDNGVRILAMVKPLSDGKGLLLVNTEFDFLQKSLEGFSKKEGGK
                     IKLVQKLGKEGQDETIINLNADNLKSSPIKVANANWYLLIEAPQKQSKVYIIIGVLFG
                     SAIAVIIGMIRLVRKFDHTLNQDMTAIQSMLKGEKPATPLNFELTKDLHGTLVANPVI
                     AATIKQEEKKSELDQLASIDHQALEMTAVDVNSLKQKQEDVPKYNEADMLSLDSIKDI
                     APQAVEKVEQKLDTNSLEYQKAQLKLAEETAIPKHIFRAYDIRGKVHSEITPALAHQI
                     GLAIGTEARNRGEATIVVGRDVRLSSGELLKSLVAGLQETGCDVIDVGEVPSPVLYYA
                     AKNIGTGSGVMVTASHNPAPDNGFKIMLGHHTLVDTEIAALRQRILDKDFVKGKGSYV
                     MRDIADDYLQAIHDDVILANGFNVVVDAGNGVAGPFAVKMLKDLGCTVSELYCTPDGS
                     FPNHDPDPSKLENLDDLLSDVAISGADLGLAFDGDGDRVVIVTGSTKVIASDKLLMLF
                     AKELLATQPGADILFDVKCSRDLVTIINTNGGRPIMTKTGHSYIKAGMEQTGALLAGE
                     MSGHFCFKDRWGGFDDGLYAAARFLEILANNFGATADELFAEFPERCVTPEIQILVDE
                     ERKHSLIETLIAQNREISGGVINTTDGLRVEFAYGWGVVRASNTGSYLVARFEANDEV
                     GLQQIKATFKELLQKADPMMLVSL"
     gene            2810..3721
                     /gene="argB"
                     /locus_tag="PLY05_02785"
     CDS             2810..3721
                     /gene="argB"
                     /locus_tag="PLY05_02785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004722746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNC02258.1"
                     /translation="MTLSRDAALNVAQVLTEALPYIQRFAGKTVVVKYGGNAMTDPEL
                     ENSFARDIVLLKTVGLNPIVVHGGGPQIGDLLTKIGKQSEFIDGMRVTDAETMDVVEM
                     VLGGLVNKSIVNLINKNGGRAMGLTGKDGNLIRAKKMYLEKTQADGSVQQVDIGQVGE
                     VAGIKTDVLEMFMASDFIPVIAPVGVGDAGESYNINADLVAGKVAEALKAEKLILLTN
                     ITGVLDKNKNILTGLSTQDVDNLIADGTIYGGMIPKISCALDAVKGGVISAHIIDGRV
                     PHAALLEIFTDEGIGTLITNRKNVRKK"
     gene            complement(3826..4908)
                     /locus_tag="PLY05_02790"
     CDS             complement(3826..4908)
                     /locus_tag="PLY05_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC02259.1"
                     /translation="MDTNNSSALWMNETLRQYALDNIGINDDQWHALCHLFSSVNSQH
                     TTINETVNYCVENIHILEKSLQNNQVLLSTVLDNMGACVYMKSLDGRYLYANNALAEV
                     VKKSPLHIIGLRDTNLFTQAVSDQLKINDQIVIDKNEKLATLETLQLINDTELQYYWS
                     VKVPLHDSSGKIYALLGMSTDMTERKKLEDNLLYLATTDELTQLYNRRQFLHLSEQTL
                     NRSKRYNEPLTLLMCDIDFFKHINDTFGHAAGDLVLQKVSQTLKSALRETDIAGRVGG
                     EEFAILLVQTPQEIGFDVAERLRKSIENCPIYLEDGINTIPITVSIGAASPIYPYETL
                     SSLMQRADQSLYQAKRSGRNQVCLAA"
     gene            5079..>5584
                     /locus_tag="PLY05_02795"
     CDS             5079..>5584
                     /locus_tag="PLY05_02795"
                     /inference="COORDINATES: protein motif:HMM:NF024836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC02260.1"
                     /translation="MMTMSAQTIYNETAFVIKNSQGAINKQPKFVAKFASNQQQILAA
                     QVLRSQTFGQEYGVSFAQGIDQDIYDEYCLHLNVYDNNTGAIIATTRLLTDDNARLLN
                     GFYSQQEFDLSLLLSQLNGRILEIGRTCVHPDYRSGAAITVLWSALAEYLLAENFNYL
                     IGCASIPLG"
     assembly_gap    5585..5684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5685..6047
                     /locus_tag="PLY05_02800"
     CDS             <5685..6047
                     /locus_tag="PLY05_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02261.1"
                     /translation="VAKQMAEHLQVGRSVLFFPEGTTTDGAGLKRFFHHLFHAACVTG
                     SDIQPVVLRYKDQRGNIHAVAPFIGDDEFSDHLLKVLQEQQIHVELKVLPRVSVYGKD
                     ERSLAKELQAMMAAALQE"
     gene            complement(6036..6500)
                     /locus_tag="PLY05_02805"
     CDS             complement(6036..6500)
                     /locus_tag="PLY05_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02262.1"
                     /translation="MFWLFSLISHALIITQLPSIFTILLITFVCTAFASLSIANYLVD
                     KRWLLSKLLKACLLASVLSFMLISAVVYGLQQAEIYHWHLAYKPMLAFAIISLPCAIW
                     LVSLKYYLEHRWPKLLLVLFISLLNNIALILLIGHAAKWATDHGYYASKLLL"
     gene            complement(6677..7501)
                     /locus_tag="PLY05_02810"
     CDS             complement(6677..7501)
                     /locus_tag="PLY05_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC02263.1"
                     /translation="MALKIHHLNCGTLCPHGAPFINGHGKITDDGKMVCHCLLIESND
                     GLVLVDTGFGIEDVKHPNQRLGYAFVAGSRPVADYRETAYMQVKELGFNPSDVRHILV
                     THLDLDHAGGLADFPHAKVHIFAPEFNAAMNPNWREKQRYKPHQWAHGVNWQTYKTEG
                     EKWQGFDSIRAIPELKDEILLVPLVGHTRGHSGVAVNTQDGWLMHCGDAYFHHNQIAA
                     DSSCPIGLDMLQYTLAANNKDRLYNTKRLQQLAQNKGHEVQLFSAHDPVELARYQH"
     gene            complement(7594..8229)
                     /locus_tag="PLY05_02815"
     CDS             complement(7594..8229)
                     /locus_tag="PLY05_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC02264.1"
                     /translation="MSHSTCQYCLSRRQFLQTGLALGTSLLLPNLSHAGGFAQSLQGE
                     TWVNGRIAHDKTRINANSFIKTTTHSASFVIGDNAFTLRPNSQVRFTPVSQQSRSVIT
                     SLRLLTGGLLSVFGKGSKQLFTPAATIGIRGTGVYMESNADSSYVCLCYGKVDLTANV
                     KAAPTNLLEATHHQAKRIQNTGAVGDETMLNHTDDELVYLESLVGRKVPFA"
     assembly_gap    8346..8445
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8446..9417)
                     /locus_tag="PLY05_02820"
     CDS             complement(<8446..9417)
                     /locus_tag="PLY05_02820"
                     /inference="COORDINATES: protein motif:HMM:NF023015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HNC02265.1"
                     /translation="MTINHLSKTPTGIRYFPDKDDIAHQLNEQHINDIVEIFNTPLQG
                     TYNWDYTSVDGRIRKLYRLGKELNWNQEMDLDWSQNYSKENYPADPNFNPFAGYAPFE
                     NLSESEKLRFAWHYQSWTLSQFLHGEQGALLVASQLVSCAPTFDAKLYASSQTFDEAR
                     HVETFNRYLQEKIGFMYEITPALKLLVDKVLADSRWDLKFIGMQIVIEGLALAAFNTM
                     RMTCLDPLLKDLIYLVTRDEARHVTFGVNYLEYIVKHLSAEEVEERAQFAYEACVVMR
                     ERLIATEVFAEFGWDVEAARRHMLDSTVMQYFNKFLFTRIIPNLSRIG"
     gene            9535..9846
                     /locus_tag="PLY05_02825"
     CDS             9535..9846
                     /locus_tag="PLY05_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HNC02266.1"
                     /translation="MSRQALLASRLQAAFAVEHLEVINESHNHRGGENAETHFKCVIV
                     SPDFIGKRAVQRHQAIYALVTDEMQKGLHALALHTYTPEEWAALGKAPDSPACQHKRE
                     V"
     gene            9850..>10169
                     /locus_tag="PLY05_02830"
     CDS             9850..>10169
                     /locus_tag="PLY05_02830"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HNC02267.1"
                     /translation="MDSYVIFKHVHMTAVALSGLLFMVRGLWLLQGSTQLQAKWVKIT
                     PHVIDTLLLVSAIAMLVVSQQFPAWVHVKITLLIVYIGLGLMAFKKAKTQGQKLTFLL
                     AAVAV"
BASE COUNT         3103 a         1672 c         2111 g         3083 t
ORIGIN      
        1 attcaagttg gtgaacgttt ggcacaatac gtgcttgtgc ctgttattca ggcagattta
       61 acagtagttg atgaatttgt agctaccgaa cgtggagctg gtggttttgg tcattctggc
      121 cgacattaat aagatttata cagttattgc ttatttgttt gtgttttaag ctcataatgg
      181 tttgatgttc agataatacg ctaattgtag agcgatgcga cgtcctaata tagtcattag
      241 gatattggcc taatacaatg atacccagat agataagatt agaattaggg gaagaaaatt
      301 ggctcagcaa aaaacggttt ttaagatggc aatgcctttt gtgataggtg ttactgtctt
      361 attgttggta attatagcgg tgattggttt tattgctatt tctgaccaaa aaagacagca
      421 agtagaagaa gctaagcaat atgaacaacg agccgttgat gtagttaata actttttaga
      481 acagcaagat gactttgcta aacaattagc tagtcagcct tctgaaatta ttgttggaaa
      541 aattgcaggt gctgtattgg cacaagtggt tctaacgggt gatgaaacga cgcctaaatc
      601 gcttagtttt agcaatcaag atttattacg tcgtgctgcg gctcaaggtg gtaatgtaac
      661 tgctgaaatg agtgttgata atggtgtgcg tattttggcg atggttaaac ccttatcaga
      721 cggtaaaggc ttattactcg ttaatactga gtttgatttt ttacaaaaaa gtttagaagg
      781 ttttagtaaa aaagagggag gaaaaatcaa attagtacaa aaattaggaa aagaaggtca
      841 agatgagaca attattaatt tgaatgctga taacttaaaa tcatcgccaa ttaaagtggc
      901 taatgctaat tggtatttgc ttattgaagc accacaaaaa caaagtaaag tttatattat
      961 tattggcgta ttgtttgggt cagcaattgc cgtgattatt ggtatgatcc gtttagtgcg
     1021 caagtttgac cacacactca atcaagatat gacggctatt caaagtatgc ttaaaggtga
     1081 aaagcctgca acaccgctta actttgaact taccaaagat ttgcatggta cgcttgtcgc
     1141 taatcctgtg attgcagcaa cgattaaaca agaagaaaag aaaagtgaat tggatcaact
     1201 ggcaagcata gatcatcaag ctttagaaat gacggctgtt gatgtcaata gtttgaagca
     1261 aaagcaagaa gatgtgccta aatataacga agccgacatg ttgtcgttgg atagtattaa
     1321 agacattgca ccacaagccg tagaaaaagt agaacaaaag cttgatacta attctctaga
     1381 ataccaaaaa gcgcaattaa aattagctga agaaacagcc atacctaaac atatctttag
     1441 ggcttatgat attcgaggta aagtacatag tgaaattacg ccagctttag cacatcaaat
     1501 tggtttggcg attggtacag aagcacgtaa tcgtggcgaa gcaacgattg tagtagggcg
     1561 agatgtgcgt ttgtcgagtg gagagttatt aaagtcttta gtcgctggtt tacaagaaac
     1621 aggttgtgat gtcattgatg tcggcgaagt cccatcacct gttctgtatt atgcagccaa
     1681 aaatattggt acgggtagtg gtgttatggt cactgctagt cataatcctg caccagataa
     1741 tggctttaaa attatgttag gccatcacac cttagttgat accgaaattg ctgctttacg
     1801 ccaacgtatc ttggacaaag atttcgttaa aggtaaaggc tcttatgtaa tgagagacat
     1861 tgccgatgat tatttacaag ctatccatga tgatgtgatt ttagctaatg gctttaatgt
     1921 ggttgttgat gctggtaatg gcgttgctgg gccttttgct gtcaaaatgt tgaaagattt
     1981 gggatgtaca gtcagcgagt tgtattgtac acctgatggt agtttcccga accatgatcc
     2041 tgatccaagt aaattagaaa atttagatga tttattgagt gacgtggcaa ttagtggtgc
     2101 agatttaggt ttggcctttg atggtgatgg tgacagagtt gttattgtga cgggttctac
     2161 caaagtaatt gccagtgata aattattgat gttgtttgct aaggagctat tagcaacaca
     2221 accaggtgcg gatattttat ttgacgttaa atgtagtcgt gatttagtca ctattattaa
     2281 cactaatggt ggtcgaccga ttatgactaa aacggggcac tcttatatta aagcaggcat
     2341 ggagcaaaca ggtgctttat tagcaggtga aatgagtggt catttctgct ttaaagatcg
     2401 ttggggtggt tttgatgatg gtttatatgc tgccgcacgt ttcttagaaa tattagcgaa
     2461 taattttggt gcaactgctg atgagttatt tgcggaattc cctgagcgtt gtgtgactcc
     2521 agaaattcaa atcttggttg atgaggagcg taaacacagt ttaatcgaaa cgttgattgc
     2581 tcaaaaccgt gaaattagtg gtggagtaat taacactaca gatggtttac gtgttgaatt
     2641 tgcgtatggt tggggcgtag tgagggcatc gaatacaggc tcttatttag ttgcccgatt
     2701 tgaggcaaat gatgaagtag gtttgcagca aattaaagca acctttaaag agcttttaca
     2761 aaaagcagac cctatgatgt tagtttcatt gtaattttag aggaaaaaca tgaccttatc
     2821 gcgtgacgca gcattaaatg ttgctcaggt tttaaccgaa gctttgccat atattcaacg
     2881 ttttgcagga aaaacggtag tcgttaaata tggtggcaat gccatgactg atccagagtt
     2941 agaaaattcg tttgctcgcg atattgtatt gttaaaaact gtgggattaa atcctatcgt
     3001 ggtgcatggt ggtgggccac aaattggtga tttattaact aaaattggta aacaatctga
     3061 atttattgat ggtatgcgtg tcactgatgc cgaaactatg gatgtggttg aaatggtctt
     3121 gggtggcttg gtcaataaat ctattgttaa tttaattaac aaaaatggcg gtcgtgccat
     3181 gggtttaaca ggtaaagatg gcaatttaat tcgcgccaaa aaaatgtacc tagaaaagac
     3241 tcaagcagat ggctcagttc aacaagttga tattggtcag gtgggtgaag ttgctggcat
     3301 caaaactgat gttttagaaa tgtttatggc cagtgatttt attcctgtga ttgcacctgt
     3361 cggtgtaggt gacgcaggtg agtcttataa cattaatgca gatttagtag caggcaaagt
     3421 tgcagaagcc ttaaaagccg aaaaactcat tttgttaact aacatcacag gtgtattaga
     3481 taaaaacaaa aatatcctaa caggtttaag tacacaggat gtcgataatc ttattgccga
     3541 tggcacaatt tatggtggta tgattcccaa aatttcctgt gcgttggatg cagtaaaagg
     3601 cggcgtgatt agtgcccata ttattgatgg gcgtgttcct catgctgctt tattagagat
     3661 ttttacggat gaaggtattg gtacattaat taccaatcgt aaaaatgtac gtaaaaaata
     3721 atcttttttg ttgcgttgac tctagctctc tcccataggg agagagctct tttacccttc
     3781 gactccgctc agggaacaga attttggcgg ctgagagtca aagttctaag ccgctaaaca
     3841 gacctgattt cgaccactac gtttggcttg atacaaagat tgatcagcac gttgcattaa
     3901 acttgataaa gtttcataag gatatattgg agaagcagca ccaatgctga cggtaattgg
     3961 gatagtattg atgccatcct ccagataaat aggacagttt tctatacttt tgcgtaaacg
     4021 ctctgccaca tcaaaaccaa tttcttgtgg ggtttgtact aataaaatag caaactcttc
     4081 accgccaaca cgtcctgcaa tatcggtttc acgaagcgca gattttaaag tttgtgatac
     4141 tttttgtaag actaaatcgc ctgctgcatg gccaaaggta tcattaatat gtttaaaaaa
     4201 atctatatca cacattaata atgttaacgg ttcgttataa cgtttgctac gattgagtgt
     4261 ttgttcagat agatgtaaaa attggcgacg attatataat tgtgttaatt catctgtcgt
     4321 tgccaaataa agcaaattat cttctagctt tttacgctct gtcatatcgg ttgacatgcc
     4381 caataacgca taaatttttc ccgatgaatc atgtaaaggg actttgacag accaataata
     4441 ttgtagctcg gtatcattaa tcagttgtaa agtttcgagc gtggccagtt tttcgttttt
     4501 atcgatgact atttgatcgt tgatttttaa ttgatcactt actgcctgag taaataaatt
     4561 agtatcgcgt aaaccaataa tatgcaaagg agattttttt acgacttccg ctaaagcatt
     4621 attcgcatat aaatagcgac catcaagaga tttcatatat acacaagctc ccatattatc
     4681 caatacggtg cttaataaga cttgattgtt ttgtaagctt ttttctagga tatgaatatt
     4741 ttcgacacaa taattaacag tctcattaat cgttgtatgc tgtgagttta cagaagaaaa
     4801 gaggtggcat aaggcgtgcc actgatcgtc attaatacca atgttatcga gtgcatattg
     4861 tcgtaaagtt tcattcatcc ataatgcaga tgagttatta gtatccatat cacgtccttt
     4921 tattaatttg aatagacaat ctatcaatat cattagtttg tcaataagga aaaaattata
     4981 caaaaaacga ttgtcctcaa agtgtcattt tttgttcatc ttcgtttcat gcaaatcact
     5041 catagtgttt gttattgaca tgatggagaa gacaaaagat gatgactatg tcagcacaaa
     5101 ccatttataa cgaaacagct tttgttataa agaatagtca aggggctatt aataaacaac
     5161 ctaaatttgt cgcaaaattt gcatcaaatc aacaacaaat tttggcagcc caagtattaa
     5221 gaagccaaac ttttggtcaa gaatatggtg tgagttttgc acaagggata gatcaagata
     5281 tttatgatga atattgtttg catctaaatg tttatgataa caatacaggc gcaattattg
     5341 ccactacacg tttattaacc gatgataacg cacgattatt aaatgggttt tattctcagc
     5401 aagagtttga tttgtcgtta ttattatccc aattaaatgg ccgtatttta gaaattggtc
     5461 gaacttgcgt acatccagat tatcgttctg gggcagcaat tactgtatta tggtcggcat
     5521 tggctgagta tttattggca gaaaatttta attatttaat tggctgcgcg tctattcctt
     5581 taggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtagcc aaacaaatgg
     5701 ctgaacattt acaagtaggg cgttcggtat tgttttttcc agaaggaaca accacagatg
     5761 gcgcagggtt aaaacgcttt tttcatcatt tatttcatgc cgcttgtgta actggttcag
     5821 atattcaacc tgttgtctta agatacaaag atcaacgtgg caatatacat gccgttgcac
     5881 cgtttattgg tgatgacgag tttagcgatc atttgttaaa agtgttacaa gaacagcaga
     5941 ttcatgttga attaaaagta ttaccgcgtg tctctgtgta tggcaaagat gaacgtagct
     6001 tggctaaaga gttacaagca atgatggcgg ccgctttaca agagtaattt actggcataa
     6061 tagccatgat cggttgccca ttttgccgca tggccaatta ataaaatcag ggcaatattg
     6121 tttaacaagc tgatgaataa taccaatagt aattttggcc aacggtgttc taaatagtat
     6181 ttaaggctaa ctaaccaaat ggcacacggt aaactaataa tggcaaaagc caacataggt
     6241 ttatatgcta aatgccaatg ataaatctcg gcttgttgta aaccataaac aacggcacta
     6301 atcaacataa aacttaaaac acttgctaac aaacacgctt ttagtagttt tgataataac
     6361 catcgtttat caactaaata attagcaata ctcaaagaag caaaggctgt gcaaacaaaa
     6421 gtaatcagta gaatggtaaa aatggatggc agttgagtga tgatgagtgc gtgactaatg
     6481 agagaaaata accaaaacat gaatatcaac acatcatcaa aaagatatgg ctagtataaa
     6541 atccaattag cttagtttat tgataataaa agacgaaatt gcctctctat agaatagtat
     6601 cgtcagttaa tttggtttcg acttcactca atcaatgcaa aaatgcgccc tgagcgaagt
     6661 cgaaaggcag tgttaattaa tgctgataac gagccaattc aacagggtca tgcgcgctaa
     6721 ataattgcac ttcatggcct ttgttttggg caagttgttg taaacgcttg gtattgtaga
     6781 gacggtcttt attattggcc gctaaggtgt attgcaacat atctaagcca atggggcagc
     6841 ttgagtcagc ggcaatttga ttatgatgaa aataggcatc gccacagtgc attaaccagc
     6901 catcttgagt gttgacggca acaccactat gaccacgggt atgaccaact aaagggacta
     6961 ataaaatttc atcttttaat tctggaatgg cacgaatact atcaaaaccc tgccattttt
     7021 cgccttcagt tttataggtt tgccagttaa caccatgcgc ccattgatgt ggcttataac
     7081 gctgtttttc gcgccaattt ggattcatcg ccgcattaaa ttcgggggca aaaatgtgaa
     7141 ctttggcgtg tggaaagtcg gctaaaccgc ctgcatgatc caagtctaaa tgtgtcacta
     7201 aaatatggcg aacatcactt gggttaaaac caagctcttt aacttgcata taagcggttt
     7261 cacgataatc agcaactgga cgcgagcctg caacaaaggc atagcctaaa cgctgattag
     7321 gatgtttgac atcttcaata ccaaatcctg tatcaactaa gactaagcca tcattacttt
     7381 caatcaacaa acaatgacaa accattttgc catcatcagt gattttgcca tgcccattga
     7441 taaatggtgc accatgaggg cagagtgtgc cacagtttaa gtgatgtatt tttaatgcca
     7501 taacataacc tattaatttt attgggatag cccaaaaatg aatgggtcat taaagggcat
     7561 aaacaaaata gcgtacagaa tatagcaagc ttattaagca aagggaactt ttcgccccac
     7621 taaactttct agataaacta attcatcgtc tgtatgatta agcattgttt catcgccaac
     7681 agcccctgtg ttctgaatac gtttggcttg atggtgtgtg gcttctagta aattggttgg
     7741 ggctgctttg acattagcgg ttaaatctac tttgccataa cataaacaca cataactact
     7801 gtctgcatta ctttccatat aaacgcctgt gccacgaatg ccaatggttg cagcaggtgt
     7861 aaacagttgt tttgatcctt tgccaaaaac actcaataag ccacctgtta ataaacgtaa
     7921 actggtaatt accgatctac tttgttggga tacaggtgta aagcgtactt ggctattagg
     7981 tcgtaaggta aaagcattat caccaatcac aaagctagca ctgtgagtgg ttgttttgat
     8041 aaagctattg gcgttaatgc gtgttttatc gtgagcaata cgaccattca cccaagtttc
     8101 tccctgtaaa ctttgggcaa aaccgcctgc atgactgaga ttaggtaaca ataaacttgt
     8161 gcctaaggct aagcctgttt gtagaaattg gcggcgagat aggcaatatt ggcaagtaga
     8221 gtgagacata ttcgtattcc tttttagagg gtatccattt gagtttttgt aatagagctt
     8281 gttacaagca gtttgagaat aattgtttag ttcttgtaag gcaataaaaa agctgtaaat
     8341 aatctnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngccga ttctcgatag
     8461 attgggaata atgcgcgtaa acaagaattt attaaaatat tgcatcacgg tcgagtcgag
     8521 catatgacgg cgtgcggctt caacatccca gccaaattcg gcaaagactt cagtggcaat
     8581 taaacgttct ctcatcacca cacatgcttc ataagcaaat tgagcgcgtt cttcaacttc
     8641 ttcagcagac agatgtttga caatatattc taagtagttg acaccaaaag tgacatgacg
     8701 agcctcatca cgagtgacta aataaattaa gtcttttaat agtgggtcta aacaggtcat
     8761 gcgcatggta ttaaatgccg ctaatgctaa accttcaatc acaatctgca taccaataaa
     8821 ttttaaatcc caacgcgaat cagccaagac tttatcaact aataatttta aggctggggt
     8881 aatttcgtac ataaaaccga ttttttcttg cagataacgg ttaaatgttt cgacatgacg
     8941 tgcttcatca aaggtttgtg aagaggcata aagtttggca tcaaaagttg gtgcgcaaga
     9001 gactaattgc gaggcgacaa gtaacgcgcc ttgttcgcca tgcaaaaatt gcgataacgt
     9061 ccatgattga taatgccaag caaaacgtaa tttttcggat tcactaagat tttcaaaagg
     9121 tgcataacct gcaaaaggat taaaattagg atcagcagga taattctctt tggagtaatt
     9181 ttgtgaccaa tctaaatcca tttcttgatt ccaattgagt tctttgccca aacgatacaa
     9241 tttacgaatg cggccatcga ctgaggtata atcccaatta taagtgcctt gtagcggagt
     9301 attaaaaatc tcaacaatat cgttgatatg ttgctcattg agttgatggg caatatcatc
     9361 tttatcagga aaataacgaa tgcctgtggg tgttttgctg aggtggttaa tggtcataat
     9421 tatttttcct gtttttgttt ggtactatgg tgctacttta tatagactaa tttgagctaa
     9481 agggcaaata attttttgtg ttagaatcga caaaattttt acgaggtcaa acaaatgtca
     9541 cgccaagcat tattagccag tcgtttgcaa gctgcttttg cggttgagca tttagaagtt
     9601 attaacgaat cgcacaatca tcgtggtggc gaaaatgccg aaacgcattt taagtgtgtg
     9661 attgtgtcgc ctgattttat tggcaaacgt gccgtacaac gtcatcaagc gatttatgct
     9721 ttagtgaccg atgaaatgca aaaaggttta catgccttag ctttgcatac ctatacgcca
     9781 gaggaatggg cggcgttggg taaagcccct gatagtcctg cttgccaaca taaacgtgag
     9841 gtgtaaaaaa tggatagtta tgtgattttt aaacatgtgc atatgacggc tgtggcatta
     9901 agtggcttat tgtttatggt gcgtggtttg tggctgttgc aaggttcaac acagttacaa
     9961 gcaaaatggg ttaaaattac gccacatgtg attgatacac tattgttggt ttcagcaatt
    10021 gccatgctag tcgtttcgca gcaatttcca gcgtgggtac acgttaaaat taccttatta
    10081 atcgtttata tcggtttagg tttgatggca tttaagaagg caaaaactca aggtcaaaaa
    10141 ttaacatttt tgctggctgc ggtggcggt
//
LOCUS       DAOQMB010000041         9979 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2632_length_9979_cov_6.727932, whole genome shotgun sequence.
ACCESSION   DAOQMB010000041 DAOQMB010000000
VERSION     DAOQMB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2632_length_9979_cov_6.727932"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..129
                     /locus_tag="PLY05_02835"
                     /pseudo
     CDS             1..129
                     /locus_tag="PLY05_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804886.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            113..1306
                     /locus_tag="PLY05_02840"
     CDS             113..1306
                     /locus_tag="PLY05_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004660851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC02268.1"
                     /translation="MKYLVDKTLKSDMDYHKQIEQAFVYRKSYLAKPIRKVSETETLS
                     KFIYQVVEKSAGTEMILGGKRVTAFQLGDYEIIKHEQGFLDGLKEVWATGSILDGNST
                     GRFFRDYLDGRNATDGLGVLYKVSDIGDDGLGYRYITGPKKSTATKGKYFQGVPLEKR
                     NLDKVENISPIENFFDLAGSFGNCRGEGGVGFRSGKKPEIYLKSILDTFSQEGDLVLD
                     SFLGSGTTAAVAHKMNRRYIGIEMGDHALSHCLPRLQKVIDGEQGGISQAVNWQGGGG
                     VKFYRLGEPIFDTQGQINPNIHFKDLAAHVWFSETKTPLQTVPDSPLLGIHNGTAYYL
                     LYNGILGDKRPNMGNVLTRQVLRDLPPHDGQKVIYGESTRVGQETLDLKQITFKQTPY
                     DIKAR"
     gene            1308..3911
                     /locus_tag="PLY05_02845"
     CDS             1308..3911
                     /locus_tag="PLY05_02845"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC02269.1"
                     /translation="MKLKKYQNNALNALKTYLQRARITDPQTAFVQFTGKNTGTIPLY
                     RHIQGLDKIPYVCLRLPTGGGKTILAAHSINIAAENYLEQEYPLVLWLVPSNTIRSQT
                     YNALRDPRHPYRQALDDTFNGKVRVFDVTQLEEIQPQDISQNVCIVIGTIQTFRVDKT
                     EGRDVYANKEALETHFNHIATKTGLEYSNEGKPKYSFANVLHLHRPLVLLDEAHNART
                     TLTFDILQRLSPACIIEFTATPDNDKKTGSNVLYRVSAGELKNEDMIKLPILLTAHST
                     WEATVRDAVANRKRLAQITQQSASPIRPLLLIQAENADKTVNVAVLKQHLIDNENINP
                     DTIAIATGNQRELEGVDLFAPDCKIEIILTVQALKEGWDCSYAYVFCSVANIRSTKDI
                     EQLLGRVLRMPFATKQAIDDLNKAYAHVCSPDFYQGAIQLKEMLTTMGFEKEEADQYV
                     NLQQPLLENTDDLPLFKPKPQLAFTVNEAPRTYDFSKQELEAIDIQPLADGQVKITVK
                     SDLPLALEQKLIAALPKKEQAQAVAVITEYKKQLEKQKSPQEQGLPFSVPMLCLLEQG
                     ELELAVKDTILELQGWSLLDYPTTLPEFSFDEDSKTFAFDIENEHIVWQLLGSQEQIM
                     LDYQQDWTEIELVEWLDRRVRQIDVQPITMLSFVQRIITHLQQQRGFSLDLLVRAKFI
                     LQKVLLEKISSYRQKSASTGFQTLLLDSPMVETSFKFNFDFSKVNYSPKAFYRGGSKF
                     PEHYFSFIADMNNEEIDCAKSFQRLHLENKLAYWVRNLESDSQNAFWLPTATGKFYPD
                     FVAMLNDGRLLVVEYKGGQLVTNDDSKIKNAIGRKWEAASQGKALFLMAVKKDENGHG
                     VYQQIKAKITG"
     gene            4139..4306
                     /locus_tag="PLY05_02850"
     CDS             4139..4306
                     /locus_tag="PLY05_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02270.1"
                     /translation="MANIENDNSLLADCSDVLRSIPLPQTQDMADMLDVLDDDNASTV
                     EKATAILNWWF"
     gene            4387..4953
                     /locus_tag="PLY05_02855"
     CDS             4387..4953
                     /locus_tag="PLY05_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02271.1"
                     /translation="MNPLETAHLTLLSSQTCSKLSATAEGKLSFNIWLDPHDQSLYLA
                     LTHNESGGYFSHELVPINTIIDCLRSLKATNKAFPAVRLRTTFQGKSANNPSFLAAVL
                     RHLNVLKACTSNTSLHLVADDLETWPTRLSQLFAAQSSDSSDSSINSANPTKNAKSRA
                     KTVNKSLLDSDKPNTAASIMETDDENHS"
     gene            4937..5308
                     /locus_tag="PLY05_02860"
     CDS             4937..5308
                     /locus_tag="PLY05_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02272.1"
                     /translation="MKIIRDIAAVQGLVKDNIRLSIEKELETLLEDFPQTYDPSIHGW
                     FIVLESINDLYAPLAMLPYSLYEKLKHQDFDWLGHHPDFYQILFTLNCNECLLVYLPK
                     QLIHALPELNRTLEQLACPVA"
     gene            5370..5651
                     /locus_tag="PLY05_02865"
     CDS             5370..5651
                     /locus_tag="PLY05_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HNC02273.1"
                     /translation="MTARKKLFALGELRITPSAGYYLPAYYIQPMDLIRRHQHGDWGD
                     ICDEDAQANQQAIIHQTRIVSSYLIGNKKVLVITEADRSSTTVLLAQDY"
     gene            6178..7356
                     /locus_tag="PLY05_02870"
     CDS             6178..7356
                     /locus_tag="PLY05_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC02274.1"
                     /translation="MASNNRILKLSKSVIDGLAASQSKEDEYTDSETSGLKLIVTKTA
                     KKSFLFRYTIHGRKRAMKIGNYPVISLVEARKKVLDAKRLLNDGVDPQAQRDNQTQTP
                     VFKTFCEDYYLPHARKHKRSFGDDECKLNKHLYHYFGQYTLNNINTLKIEQYLSSLRD
                     KCHLAPATTNRHLALLSVIFNLAISLNFLEKNPCAPIRKLKEHNERQRYLTSDETQRL
                     LAVMDDTNPETCEPNRAAVAAIKLLLLTGTRREEALNAKWEHINFEQRTWLLPKTKND
                     RIRHVQLNEAAIILLKSIKKIDDCPYVFVNERTKNRLNTPVKAFKRLLAKANIKDFRI
                     HDLRHSFASAAVNSGSSLYIVQHLLGHASIQTTQRYAHLKNETLLAASENVAKAIQNL
                     "
     gene            7500..9596
                     /locus_tag="PLY05_02875"
     CDS             7500..9596
                     /locus_tag="PLY05_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02275.1"
                     /translation="MLKNTLTLAENEFIINKINTLDAGCTLIESPTGTGKTTFVLEHL
                     TQSTTILMLVPLVLQVRQLKSQYGQRTDLVFLSGTDKDSTNNKSLSDLQGKHIVATYD
                     LWPALKQNLSFENYTLVVDEVHKMYSAGSYRDEALNPILDIINNKTLFSKRLLLTATY
                     TPLLANLANIIPDSHITILRAQAATKKLTIRSYEDKWSYHWLKFVINRLQTSKPQNKV
                     IMVRLNSNSRMDKAIQVLEKLNFKVLGVSRKTIGQREVKMLIENQKLPQKYDVILTTS
                     ILDEAINLNNDDSEIDSVHIVDASAHPEEIVQFMGRLRVANPPFFLHINKTDNFFTQL
                     STNNVDEYESKIKSKYTTLENFAHASKQLAIDFDNQLSPFEIFKGFNIVNTTLQNFLE
                     CPILTVQNQTVSANVAGILACCYKLDIFHAFKHYEYLKTRLTSLLPTLVVVKESVLDK
                     KDDNLQSIIDNAEIDKVQKFSEAVKKVCQHIKDDLAKSNKTLLNYAQEKNKILDKNPS
                     QGNPFNPNNHPIEYTTYFTAIDLSTHLTRIEDVERALINKHEYKVIELSKSYRTDFFK
                     NDIRKSLQQRFDDNNLIIKPKDAVDIVLQAFRNTRQKFTSLNSIKDCKHLGIKINSGN
                     QHISIAESKALKLIKDLVDVTDTNSHKPQKHYLQLVNMTWNNYQFNNASRAPAYENLK
                     AKEPPVTTGNLEDEFA"
BASE COUNT         3347 a         1960 c         1801 g         2871 t
ORIGIN      
        1 cttagtatga tgtacccgcg tttggtgttg ttgcgtgagt tgttggcaga agatggtttt
       61 atcgtttgtc atattgatga ttcggaaagt cattatttga aagtattaat ggatgaagta
      121 tttggtagat aaaacactta aatcagatat ggactatcac aaacaaatag aacaagcatt
      181 cgtctatcgt aaatcttatt tagccaagcc cattcgtaaa gtttcagaaa ctgaaacact
      241 atcaaagttc atttaccaag tagtcgaaaa atccgcaggg acagaaatga tactaggtgg
      301 taagcgagtg acagcttttc aattaggcga ttatgaaatc atcaaacatg aacaaggttt
      361 tcttgatggc ttaaaggaag tttgggcaac gggtagtatt ttggatggta attcgacagg
      421 acgttttttt agagattatc ttgatggtcg aaatgcaacg gatggattag gtgttttata
      481 taaagtgtcg gatataggcg atgatggatt aggttatcgc tacattacag ggccgaaaaa
      541 aagtacagca acaaaaggta aatattttca aggtgttcct ttagaaaaac gtaacttaga
      601 taaagtcgaa aacatttcgc ctatagaaaa cttttttgac ttggctggta gttttggtaa
      661 ttgtcggggc gagggtggag ttggctttag gtcaggaaaa aagcctgaaa tatacctcaa
      721 atccatctta gatacgtttt ctcaagaagg cgatttagtc ctagacagct ttttaggcag
      781 tggcacaact gctgccgtag ctcacaaaat gaatcgccgt tatattggta tagaaatggg
      841 cgaccacgcc ctcagccatt gtttgccacg tttgcaaaaa gtcatcgacg gcgaacaagg
      901 cggcatcagc caagccgtaa attggcaagg tggtggcggt gttaagtttt atcggctagg
      961 tgaacccatt tttgacacac aaggccaaat aaaccccaac atacacttta aagacctcgc
     1021 cgcccacgtt tggtttagcg aaaccaaaac accactacaa accgtacccg acagtcccct
     1081 attaggcata cacaacggca ctgcttatta cctgttatat aacggtattt taggcgacaa
     1141 acgccctaac atgggcaacg tcttaacccg tcaagtcttg cgagatttac cgcctcatga
     1201 cggccaaaaa gtgatttacg gcgaaagtac ccgtgttggc caagaaacat tagacctaaa
     1261 acaaatcacc tttaagcaaa ctccttacga cattaaggcg cgttaatatg aaactcaaaa
     1321 aatatcaaaa taacgcactc aatgccctaa aaacctattt gcaaagagcg cgtattaccg
     1381 acccacaaac cgcctttgta cagtttacag gcaaaaacac agggacaatt ccgttatatc
     1441 gacacattca aggcttagat aaaattcctt atgtgtgctt acgtttaccc acaggtggtg
     1501 gtaaaacgat tttagcggca catagtatta acatcgccgc cgaaaattac cttgagcaag
     1561 aatatcccct cgtgctttgg ctcgtgccta gcaacactat tcgtagccaa acttataacg
     1621 ccctgcgtga cccaagacac ccctaccgcc aagccctaga cgatactttt aatggcaaag
     1681 ttcgtgtgtt tgacgtaacg caattagaag aaattcaacc gcaagatatt agccaaaacg
     1741 tttgtattgt cattggtaca atacaaacgt ttagagtcga taaaaccgaa ggccgagacg
     1801 tttatgccaa taaagaagcc ttagaaacac attttaatca tatcgcaacc aaaacaggct
     1861 tagagtattc aaacgaagga aagcccaagt attcttttgc caatgttttg catttacatc
     1921 gtcctttagt attgctagac gaagcccaca acgcccgaac gacactcact tttgatattc
     1981 ttcagcgtct atctcctgcc tgtattattg agtttaccgc cacaccagac aacgacaaaa
     2041 aaacaggcag taatgtcttg tatcgtgtat ccgcaggtga actcaaaaac gaagacatga
     2101 ttaaactgcc cattttgcta acagcacata gcacatggga agccaccgtc agagatgccg
     2161 tggctaatcg caaacgcttg gcgcaaatta cccaacaaag cgcgtctcct attcgtcctt
     2221 tattgctaat tcaagccgaa aatgccgaca aaacggtaaa tgttgcggta ttaaaacaac
     2281 atttaatcga caacgaaaat atcaatcctg acaccatcgc cattgccaca ggcaatcagc
     2341 gtgagctaga aggcgtagat ttatttgccc ctgattgcaa aatagaaatt attctcaccg
     2401 ttcaagccct taaagaaggg tgggattgtt cctatgctta tgtattttgt tcggtagcca
     2461 acattcgctc tactaaagat attgagcagt tacttggccg tgtgttgcgt atgccttttg
     2521 ccaccaaaca agcgattgat gacttaaata aagcctatgc ccacgtttgc agccctgatt
     2581 tttatcaagg ggcgatacaa ttaaaagaaa tgctaacaac aatgggcttt gaaaaagaag
     2641 aagccgacca atatgttaat ttacaacaac ccttattaga aaataccgat gacctgccac
     2701 tgtttaaacc caaaccacaa ctcgcgttta cagtaaacga agcaccacga acctatgact
     2761 ttagcaagca agaacttgaa gccatagaca ttcaaccttt agctgatggc caagtgaaaa
     2821 ttaccgtcaa gtcagactta cctttagctc tagaacaaaa actgattgcc gcgttgccca
     2881 agaaagaaca agcgcaagca gtagcggtga ttactgaata taaaaagcaa ttagaaaagc
     2941 aaaaatcccc tcaagaacaa ggcttaccgt ttagtgtgcc gatgttgtgc ttgttggaac
     3001 aaggggaatt agagcttgca gtaaaagaca cgatattaga gctacaaggt tggagcttat
     3061 tagactatcc cacaacatta ccagaattta gttttgatga agacagtaaa acctttgcgt
     3121 ttgatataga aaatgagcat atcgtttggc agttacttgg ctcgcaagag cagattatgc
     3181 tcgactatca acaagactgg actgagattg aactggttga atggttagat agacgtgtac
     3241 gacaaataga tgtgcaaccc atcaccatgc taagttttgt acagcgtatt attacccacc
     3301 ttcaacaaca acgtggcttt agcttagatt tattggtacg cgcaaaattc attctgcaaa
     3361 aagtgctatt agaaaaaatc agctcttatc gtcaaaaatc agcttcaaca ggctttcaaa
     3421 ccttgctgtt agatagtccg atggtcgaaa catcatttaa gtttaatttt gattttagta
     3481 aggtcaatta ttcaccgaaa gcattttatc gtggaggctc aaaatttccc gaacattact
     3541 tttcctttat tgcggacatg aataacgaag aaattgactg tgccaaaagt tttcagcgac
     3601 tacatctaga aaacaaatta gcttactggg tacgaaactt ggaaagtgac tctcaaaatg
     3661 cattttggct acccacagca acaggtaagt tttaccctga ttttgtggca atgctcaacg
     3721 atggccgttt attggtcgtg gaatataaag gtggtcagtt ggtgacgaat gatgatagta
     3781 aaataaaaaa tgccataggc cgcaaatggg aggccgcgag tcagggcaaa gccttgtttt
     3841 tgatggctgt gaaaaaagac gaaaatggtc atggcgttta tcaacagata aaagcgaaga
     3901 taacgggata atgccaaata taaatcgtga tgattttgaa aagtctgttt tagaggaaac
     3961 aaacgcatct caagggtaat taacctgatt taagaaacag ctaaagcaaa tctttactta
     4021 tccgtaagag cttcactttg acacatatat cattttaatg gtgtacacct gcaccatttc
     4081 ccctcaatca aacgccttac ttgttttgca agtagggcgt ttttatttgg atgattttat
     4141 ggccaacatc gaaaatgaca atagcctttt agcggactgt agcgatgtat tacgcagcat
     4201 tcccctacct caaacgcaag acatggccga tatgctagat gttcttgacg atgacaacgc
     4261 atcaaccgtt gaaaaagcca ctgctatctt aaattggtgg ttttaatcaa ctcaacccct
     4321 taaatcaaat gattaagcct tattccttgt gcaaaggaat aaggcttttt tatgaggtga
     4381 tattttatga atcctttaga aacagcgcat ttaaccttgc tgtcctctca aacttgctca
     4441 aaactttctg caacggctga gggtaaactc agcttcaata tttggcttga ccctcacgac
     4501 cagtctttat accttgcact gacacacaat gaaagtggtg gttactttag ccatgagcta
     4561 gtgcctatca atactattat tgattgttta aggtcattaa aagccactaa taaagccttt
     4621 cctgctgtgc gtttacgtac gacctttcaa ggaaaatcag ctaataaccc tagcttttta
     4681 gcggctgtat taagacatct taacgtactc aaagcctgta caagtaatac atcactacat
     4741 ttggttgctg atgaccttga aacatggcca acacgtttaa gccaactttt tgccgctcaa
     4801 agcagtgatt caagcgatag ctctataaat agtgcgaatc caactaaaaa cgctaaatca
     4861 cgcgcaaaaa ccgtcaataa aagtctttta gatagtgaca agcctaatac ggctgcctca
     4921 attatggaga ctgacgatga aaatcattcg tgatattgcc gctgtacaag gtttagtaaa
     4981 agacaatatc cgtttaagca ttgaaaaaga gcttgaaaca ttattggagg acttccccca
     5041 aacctatgac cctagtattc atgggtggtt tattgtccta gaaagcatca atgatttgta
     5101 tgctccgttg gcaatgctgc cctactcctt gtatgaaaaa ctcaagcatc aagactttga
     5161 ttggttgggt catcatcctg atttttacca aatactgttc acactcaatt gcaatgaatg
     5221 tttgttggtg tatttgccca agcaacttat tcatgcttta cctgaactta accgcacctt
     5281 agaacaactt gcctgtccag tcgcataaca aaggactgca agaagatatt caacgcttta
     5341 gtgttacttt gacattatga gaggttttta tgactgctcg aaagaaatta tttgcacttg
     5401 gagaattacg aatcacccca agtgcagggt attacttacc agcttattac attcagccaa
     5461 tggacttaat tagacggcat cagcacggtg attggggtga catttgtgat gaagatgcac
     5521 aagccaatca acaagccatc atccaccaaa cacggatagt ctctagttat cttattggca
     5581 ataaaaaagt cttagttatt acagaggctg accgttcttc aacaacggta cttttagcac
     5641 aagactacta agaccgacca gaaatgaacc agacagcaaa gaaaggcttt gctgtttatt
     5701 tcttagccta aagtattacc aagatatgat gttgatagtc taaaactggt tcttaggtct
     5761 agagattcac cgctcaaatc accaaatttt ttgaggagat gattactctc ataagacagc
     5821 ttacaaaata attttgagta agaggctata caaaaaaatc actttttccc tttaaagtta
     5881 agcacaaagc tttttagaga tatgcaataa gtgatttttt aggcttataa aaacttgact
     5941 acttttttaa ccgttatatc atcggccaat gactcgacaa gtttcgtggt catttaattg
     6001 atattcttcg gtactaataa atctaattag agtcagtgtc tttgtactca tgactcatct
     6061 aataagcctt gtgcgctcta gtattactca taaatctgca attaattaac tttcatcatt
     6121 acatggtcat ccatgtctta accttttctt ttcctagtga aaaattggag tttttatatg
     6181 gcttcaaaca atcgtatttt aaaactgtct aagtctgtta ttgacggctt agctgcaagc
     6241 caaagtaaag aagatgaata tacagacagt gaaacctctg gcttaaaact gattgtcacc
     6301 aaaacagcaa aaaagtcttt tttgtttaga tacacgattc atggccgaaa gcgagcaatg
     6361 aaaatcggta attatcctgt tattagttta gtagaagctc gtaaaaaagt tttggatgct
     6421 aaacgactgc ttaatgacgg tgttgaccca caagctcaac gagataatca gactcaaaca
     6481 cctgtattca aaacattttg tgaggattat tacttacctc atgctagaaa gcacaaacgc
     6541 tcctttggtg atgatgagtg taagttaaat aagcatcttt atcattactt tggccaatat
     6601 acgttaaaca acattaacac gctaaaaatt gagcaatatc taagctcatt gcgtgacaaa
     6661 tgtcatttag ctcctgcaac aactaatcga catcttgcct tgttatcggt catttttaac
     6721 ttggcaatca gtcttaattt tttagagaaa aacccttgtg cgccgattcg taaactcaaa
     6781 gagcataatg aacgtcagcg ttaccttacg tctgacgaga cacaacgatt attggccgtt
     6841 atggatgata ccaatcccga aacctgtgaa cccaatcgtg ctgccgttgc tgcaattaaa
     6901 ctacttcttc taactggtac aaggcgagaa gaagcgttaa atgcaaaatg ggaacacatc
     6961 aactttgaac aacgtacttg gttgttgcct aaaactaaaa atgaccgaat acgtcatgta
     7021 caacttaatg aagcagctat tattttattg aaaagtatca agaaaataga tgattgccct
     7081 tatgtttttg taaatgaacg caccaaaaac cgtcttaata ctccagttaa ggcattcaaa
     7141 cgtttgttag ctaaagccaa tattaaggac tttagaatcc atgacttaag gcatagcttt
     7201 gcttcagcag ccgtaaatag tggttctagt ttgtatatcg tgcaacacct tcttggtcat
     7261 gccagtattc aaacaacaca acgctatgct catctcaaaa atgaaacgct attagctgct
     7321 tctgagaatg tggctaaagc gattcaaaat ttataacaca gaccaagaca aaaagggcaa
     7381 tttgcccttt ttcgtcttat tttttactaa tttttaattt tttatctgaa atcatatttt
     7441 aaataaattg aaacaatcga atccttttta tcaaaaacca aaaaatctga gtaacaatca
     7501 tgttaaaaaa cacattaact cttgcagaaa acgaatttat tatcaataaa atcaatacac
     7561 tagatgcagg ctgcacattg atagagtcac ctacaggaac gggtaaaaca acttttgtat
     7621 tggagcattt gacccaatca acaacaattt tgatgttagt tccgttagtc ctgcaagtaa
     7681 gacaactaaa atcacagtat ggccagcgta cagatttagt atttctgtca ggtacagata
     7741 aggacagtac aaacaataaa tcgttatctg acttacaagg caaacatatt gttgctacat
     7801 atgatttatg gcctgctctc aaacaaaatc tatcttttga aaattacacg ttagtcgtcg
     7861 atgaagtaca taagatgtac tcagcaggtt cttatcgtga tgaggcatta aatcccatct
     7921 tagatattat caacaataaa acattatttt ctaagcgatt gttattaaca gcaacatata
     7981 cccctctttt agcaaattta gccaacatta ttcctgacag ccatattact attttacgcg
     8041 cacaagctgc gacaaaaaaa ctaactatta gaagctatga agataagtgg tcttatcatt
     8101 ggttaaaatt tgtgattaac agactgcaaa cgtctaaacc acaaaataaa gtcatcatgg
     8161 tacgtttaaa ttcaaatagc cgcatggaca aagctattca agtgttagaa aaattaaatt
     8221 ttaaagtctt aggtgttagc cgcaaaacaa ttggtcaaag agaggttaaa atgctcattg
     8281 aaaatcagaa gctaccgcaa aaatatgatg ttattttgac aacttctata ttagatgaag
     8341 ctattaacct gaataacgac gattcagaaa ttgactctgt gcatatcgtt gatgcgtctg
     8401 ctcaccctga agaaattgtg cagtttatgg ggcgattaag agtagctaac cctcctttct
     8461 ttttacatat caataaaaca gataattttt ttactcaatt atcaactaat aatgtggatg
     8521 aatacgaaag caaaatcaag tctaaatata caacgcttga gaactttgca cacgcttcaa
     8581 agcaacttgc tatagacttt gacaaccaat taagtccatt tgaaatattc aaaggcttta
     8641 atatcgttaa tacgacacta caaaactttc tcgaatgccc aatcttaaca gtccaaaacc
     8701 aaacagtgag tgcaaatgtt gctggtattt tagcctgttg ttacaagctt gatatttttc
     8761 atgcgttcaa acattatgaa tatttaaaaa ctcgtttgac ttcactatta ccaaccttag
     8821 ttgtcgtaaa agagagtgtt ttggacaaaa aagatgacaa tctgcaatct attattgata
     8881 acgctgaaat tgataaagtg caaaagttta gtgaagctgt gaaaaaagtt tgtcaacata
     8941 tcaaagatga tttagcaaaa tcaaataaaa cactcctaaa ttatgctcaa gaaaagaata
     9001 aaattctaga caaaaacccc agtcaaggta atccatttaa cccgaataac catccaattg
     9061 agtacacaac ttattttact gctattgatt tatccactca tttaacacga attgaagatg
     9121 ttgaaagagc attgataaac aaacatgagt ataaagtcat tgagttatct aagtcatatc
     9181 gtactgactt ttttaaaaat gatattcgta aatcgctaca acaacgtttt gacgataata
     9241 atctgataat caagccaaaa gatgctgttg atattgttct acaggcattt agaaatacta
     9301 gacagaagtt tactagctta aactcaataa aagactgcaa acatcttggc attaaaatta
     9361 atagcggcaa ccaacacatt tctattgctg aatctaaagc actaaaactg attaaagact
     9421 tagttgatgt tacagacact aacagtcata aaccccaaaa acattacctg cagcttgtga
     9481 atatgacttg gaacaactac caattcaaca atgctagccg cgcccctgct tatgaaaacc
     9541 ttaaagcgaa agagccgcct gtgactactg gtaacttaga agatgagttt gcgtagtgct
     9601 tgatacccag cttaaatgca gggtgttaat tagcaatgtt taaatattat tcattttttt
     9661 aatcatgtaa tcaaaatatc agtttgaaaa aataataata tttaaacatc ataatttggg
     9721 ggtgggggaa aaaggagatt ttgtgacaaa aaccatttgt gcttggtctt tattgacgaa
     9781 atgcaaacat catgggggaa tttccctaaa aacaatacta ctcacggcaa aacaccccct
     9841 gtggctatgc actcaagtat tgctcaatca ccaaaggcaa caaccttgtg tttaattaac
     9901 ttcatcagca aatcattatt agttaaaagc gtcatgctat ctcatgcgat tatttgaatt
     9961 tattaacatt aaaaagggt
//
LOCUS       DAOQMB010000042         9928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2650_length_9928_cov_13.349640, whole genome shotgun sequence.
ACCESSION   DAOQMB010000042 DAOQMB010000000
VERSION     DAOQMB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_9928_cov_13.349640"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /gene="tusA"
                     /locus_tag="PLY05_02880"
     CDS             complement(<1..165)
                     /gene="tusA"
                     /locus_tag="PLY05_02880"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HNC02276.1"
                     /translation="MTLEIKHQLDARGLNCPEPVMMLHKIVRQMQAGELLAVYATDPS
                     TQRDIPKFCQF"
     gene            complement(325..1566)
                     /locus_tag="PLY05_02885"
     CDS             complement(325..1566)
                     /locus_tag="PLY05_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC02277.1"
                     /translation="MTNHLWADKRFWVLLTLYMAQGLPTGIFSQGLPAILRSYDVSLK
                     VIGFTSLLAIPWALKFLWAPYIDKYYSTRIGQSRTWILPMQLLSMVLLLAIAFFEPKS
                     LATQQGLIYFLALMFLLNLVAASQDVATDGLAVRNLNYEERSWGNGVQVAGYRLGLII
                     GGGFLLYLVGVWQWQAAFLLMTALLLLISLPIWFYKEQAKPKTTKENYDVNYRQVFIS
                     FMAQKGFKPWLGVLITYKVGESLGSAMVKPMFVDMGLKLEQIGLFVSVIGSVATLLGA
                     ALAGWWVKRLGRYLSLLYFGVAQAFGVACYAYLSWQWQDHQQVVLWQIYAINAIEHFM
                     SGMAIVALLAAVMDYARHQHAGTDFTIQVSILTIFGGSASLAAGFLAHSVGYTAYYLI
                     SAALALVFLWPVVLWGRWLKA"
     gene            complement(1573..>1752)
                     /locus_tag="PLY05_02890"
     CDS             complement(1573..>1752)
                     /locus_tag="PLY05_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNC02278.1"
                     /translation="DLDQGPIIEQAVERVSHGDNVETLRELGEDVERNVLARAVRWHL
                     EDRVIVHGNKTIVFA"
     assembly_gap    1753..1852
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1853..2597)
                     /gene="purU"
                     /locus_tag="PLY05_02895"
     CDS             complement(<1853..2597)
                     /gene="purU"
                     /locus_tag="PLY05_02895"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004855135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HNC02279.1"
                     /translation="MKASINASIARLLITCPDKAGIVSAVSNFLYNHGANITALDQYS
                     TAPEGGTYFMRLEFQTPHLDLSKQVLAQTFEEAVAKRYNMTWQLSYADDVKKMAVLVS
                     KYDHALLELLWRWSRGGLPCQITAVISNHADLQESVERFSVPFHVVQVTKDNKQEAEA
                     QIQALLKNNDLVVLARYMQILSADFVSQWPHKIINIHHSFLPAFVGANPYQQAYDKGV
                     KLIGATAHYVTADLDQGPIIEQAVERVSHG"
     gene            2830..3348
                     /locus_tag="PLY05_02900"
     CDS             2830..3348
                     /locus_tag="PLY05_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNC02280.1"
                     /translation="MKRLTVALLAAAISTPILAAEKPYVGIDYQLGTYTHKKTDVNPE
                     TLRLRAGTEINKSLAVEAQAAFGTGGDTYVATGNVKYDIKVDSFYSIFLKPQLQISKS
                     ITAYGLLGGTYIDVSASSAGKADDTGFTHTFSYGAGADFAIKNGIRLGADFIQYMDDY
                     NAISVGIRIPIQ"
     gene            complement(3460..3648)
                     /locus_tag="PLY05_02905"
     CDS             complement(3460..3648)
                     /locus_tag="PLY05_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02281.1"
                     /translation="MNTSENISAEQSPQTTKTLTALLAESLQSKKEATNYPQPNKDRF
                     SQRPSRPPHGTRRSMGKR"
     gene            complement(3908..4924)
                     /gene="ilvC"
                     /locus_tag="PLY05_02910"
     CDS             complement(3908..4924)
                     /gene="ilvC"
                     /locus_tag="PLY05_02910"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNC02282.1"
                     /translation="MQVYYDKDADLSIIRGKKVTIVGYGSQGHAHAQNLTDSGVDVTV
                     GLRKGGASWAKVEKAGIKVAEVAEAVKSADVVMILLPDENIPEVYRNDIEPNIKQGAT
                     LAFAHGFNVHYNQVVPRADLDVIMVAPKGPGHTVRSEYLKGGGVPSLIAVYQDKSGNS
                     KQIALSYAAANGGTKGGVIETNFREETETDLFGEQAVLCGGAVELVKMGFETLTEAGY
                     APEMAYFECLHELKLIVDLMYEGGIANMNYSISNNAEYGEYVTGQRVINTQAREAMRE
                     CLRNIQNGEYAKRFILEGRTNYPEMTARRRLNADHPIEKVGAQLRAMMPWIHKNKLVD
                     QTRN"
     gene            complement(5068..>5555)
                     /gene="ilvN"
                     /locus_tag="PLY05_02915"
     CDS             complement(5068..>5555)
                     /gene="ilvN"
                     /locus_tag="PLY05_02915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNC02283.1"
                     /translation="HIISVLIENEAGALSRLVGLFSQRGYNIETLTVAPTDDATLSRL
                     TLTTSGDDHKIEQVTKQLNKLVEVVKVVDLSEGDHIERELMLIKVKAMGAIRAEIKRT
                     ADIFRGQIVDVTSSTYTIQLAGTSDKLDAFIEALSETTILEVVRSGVSGIARGEKVLS
                     I"
     assembly_gap    5556..5655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5717..7441)
                     /locus_tag="PLY05_02920"
     CDS             complement(5717..7441)
                     /locus_tag="PLY05_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HNC02284.1"
                     /translation="MELLSGGEMLIRALADEGVEFVFGYPGGAVLHIYDAIFQQDRVQ
                     HILVRHEQAAGHMADGYSRSTGKTGVVLVTSGPGATNTVTPIATAYMDSIPMVVISGQ
                     VPSHLIGEDAFQETDMIGVSRPIVKHSFQIRHASEIPAVVKKAFYLASSGRPGPVVID
                     IPKSCTAPNEKFPYEYPEKVKLRSYNPPSRGHAGQIKKAVDELIQAKRPIIYTGGGVV
                     QGNASALLVELAQLLNYPVTNTLMGLGAYPGSDRQFVGMLGMHGTYEANMTMHHADLI
                     LAIGARFDDRVTNNPKKFCTQAKVIHIDIDPATISKTITAHIPIVGAVEPVLTEMLAQ
                     LKQMGVNKPNPEAIAAWWKQIDAWRAHHGLRYEPAKAGEMKPQEVVEMLYKVTEGKAI
                     VTSDVGQHQMFAALYYKYDRPRQWINSGGLGTMGFGLPAAMGCLMAFPDQPVVCITGE
                     ASIQMNIQELSTCLQYNLPVKILNLNNRALGMVKQWQDMQYEGRHSNSYMESLPDFVK
                     LVEAYGHVGMKITDPNELESKMREAFAMKDRLVFMDVYVDQFEHVYPMLIANQSMRDM
                     WLSKTERT"
     gene            8074..>8329
                     /locus_tag="PLY05_02925"
     CDS             8074..>8329
                     /locus_tag="PLY05_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC02285.1"
                     /translation="MNKKQKMISILSIFTSAVLSASIVSADEDGAIYKWVDERGVTNY
                     SETPPDAKGANAKAQSININRYVPRGSENAVKQLEQQRTEK"
     assembly_gap    8330..8429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8433..9281)
                     /gene="rlmJ"
                     /locus_tag="PLY05_02930"
     CDS             complement(8433..9281)
                     /gene="rlmJ"
                     /locus_tag="PLY05_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HNC02286.1"
                     /translation="MNYRHHYHAGNFADVMKHILEIGLLQALHKKDNAFCYIDTHAGR
                     GWYDLAAAPAQKTGEYLEGIRKIDTRQAKPSKWVETYLENFETLRTHFGSKAYYGGSP
                     WFAIQNIREQDRMVLMEWHPVDVEKLKDNFYRHPQVAVHHRDAYEGLNALIPPKEKRG
                     LVLIDPPYEEERHDYPQVVELLAKAYDKWPTGMYAIWYPIKDRASIERFERRLVKTGI
                     RKQLVCELCVMPDDNNLGLNGCGLLIVNPPYLFAEEADKVLQWLLPQLSQNKLAKASV
                     RWLVGE"
     gene            9280..9906
                     /locus_tag="PLY05_02935"
     CDS             9280..9906
                     /locus_tag="PLY05_02935"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HNC02287.1"
                     /translation="MQSSQNQQFNKSVFNLNQAFDALATDHYAVIPQFLTSTMREALL
                     AELLQLQAQGLFHEASVGRGVGQTRQAHIRGDSICWLEDHFTIGGQYLALMEQLRQQL
                     NADFYLGLRSYEAHYAHYQAGSVYQRHVDRHRDNNARVVTTVCYLNDDWPTEAGGQLK
                     LYNPNHELLLEQTPTGGSLVLFMSADMPHEVLAANRSRFSIAGWFRQD"
BASE COUNT         2907 a         2119 c         1781 g         2821 t
ORIGIN      
        1 aaactggcaa aacttaggaa tatcgcgttg tgttgagggg tctgtggcat aaactgctaa
       61 cagctcccct gcttgcattt gtcgaacgat tttatgtagc atcatcacag gttctggaca
      121 gtttaaacca cgcgcatcta attgatgttt aatttctaaa gtcatcgttt tctcttttcg
      181 caggctttta gccaacttct ctcgtcaaaa aaaatcgtag gatgggttga cgtaggaaac
      241 ccattattta tcaagatgct tgatgggttt cgttcctcaa cccatcctac taaaatttcc
      301 atcttccaca gacaaggccg ttatctaagc ttttaaccaa cgtccccaca acacaacagg
      361 ccataaaaac actaaagcta atgccgcaga tattaaataa taagcggtat aaccaacact
      421 atgcgccaag aatcctgctg ccaaactcgc tgaaccacca aaaatcgtta aaatactcac
      481 ttgaatggta aagtctgtgc ctgcatgttg atgtcgagca tagtccatca ctgccgccaa
      541 taaagcgaca atcgccatgc cactcataaa atgctcaatc gcgttaatgg cataaatttg
      601 ccacaatacc acttgttgat gatcttgcca ttgccatgat aaataggcat aacacgctac
      661 accaaaagct tgagccacac caaaatacag taatgacaaa taacgcccta aacgtttaac
      721 ccaccatcct gccaaggctg cacctaacag cgttgccacc gaaccaatga cactcacaaa
      781 caaaccaatt tgctcaagct ttaaacccat atcaacaaac atcggtttaa ccatcgccga
      841 gcctaaactt tcacccactt tataagtgat taatacgcca agccacggtt taaaaccttt
      901 ttgtgccata aaactgatga atacctgacg ataattaaca tcatagtttt ctttggttgt
      961 tttgggttta gcttgttctt tataaaacca aatcggcaaa gaaatcagca ataacagcgc
     1021 agtcatcaac aaaaatgcgg cttgccactg ccataccccc actaaataca ataaaaagcc
     1081 accaccaata atcaatccta aacgatagcc agccacttgt acgccattac cccaactacg
     1141 ttcttcatag ttaagattgc gtaccgctag gccgtctgtg gccacatctt gactagctgc
     1201 caccaaattg agcaaaaaca tcaatgccaa aaaataaata agaccttgtt gtgtagccaa
     1261 actttttggc tcaaaaaaag caatcgccaa taacaacacc atagataaca actgcatggg
     1321 caaaatccat gttcgtgatt ggcctatgcg tgttgaataa tatttatcaa tgtagggcgc
     1381 ccataaaaac tttaacgccc acggaatcgc taataaactg gtaaaaccaa tcacttttag
     1441 cgacacatca taactacgca aaatagcagg taatccttga ctaaaaatgc ctgttggtaa
     1501 accctgtgcc atatacaagg tcaacaatac ccaaaagcgc ttatccgccc ataaatgatt
     1561 ggtcatagtt tattacgcaa aaacaatggt tttattgcca tgtacaatca cacggtcttc
     1621 taaatgccaa cgcactgcac gtgctaagac attacgctca acatcttctc ctaactcgcg
     1681 taaggtctca acattatcac cgtgggacac tcgctcaacc gcttgttcaa taatcggccc
     1741 ttgatctaaa tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaccgtgg
     1861 gacactcgct caaccgcttg ttcaataatc ggcccttgat ctaaatcggc agtgacataa
     1921 tgtgccgttg ccccaattaa tttaacgcct ttatcgtaag cttgctgata ggggtttgcc
     1981 ccgacaaaag caggcaaaaa cgagtgatga atattaataa ttttatgtgg ccactgactg
     2041 acaaagtcag cacttaaaat ttgcatataa cgcgctaaca ccactaagtc attatttttg
     2101 agcaaggctt ggatttgtgc ttctgcttct tgtttattgt ctttagtcac ttgtaccaca
     2161 tgaaatggca cactaaaacg ttcaacagat tcttgcaaat ctgcatgatt actaattact
     2221 gcggtaattt gacagggcaa acctccacgt gaccaacgcc acaataactc taataaagca
     2281 tgatcatatt tagacactaa taccgccatt tttttgacat catcggcata acttaactgc
     2341 caagtcatgt tgtaacgttt agccacggcc tcttcaaagg tctgagccaa tacttgttta
     2401 gataaatcta aatgtggggt ttgaaactcc aaacgcataa aatatgtgcc accttcaggt
     2461 gctgtggagt attggtctaa agcggtgata tttgcaccat gattatagag aaaattcgat
     2521 actgcactga caattcctgc tttatcaggg caagtaatta acagacgtgc aatactcgca
     2581 tttatagaag ctttcataaa aaattttgac cttattcaat cattttaaga cgattattag
     2641 cgcatagtgt acaataaacc aagcttatat gtattttgtg aaatctttgc cttgacaata
     2701 agccattttt tgcaaattat cgctataact tttaaggctg acgattttaa tggtttttaa
     2761 cattttcgtt atcaataatt tcgtttgtct ccactttgga actaccacta acaacatgca
     2821 ggaagaacaa tgaaacgcct cactgttgct ttattagctg ctgctatcag cacccctatt
     2881 ttggccgccg aaaaacctta tgtcggcatt gattatcaat tgggtacata tactcataag
     2941 aaaacagatg tcaatcctga aactctgcgt ttacgtgctg gcactgaaat taataagtct
     3001 cttgccgtag aagcacaagc tgcttttggt actggtgggg atacctatgt tgcaacgggt
     3061 aatgttaaat atgacatcaa agttgacagt ttttacagta tttttttaaa accacaacta
     3121 caaattagca aaagcatcac tgcttatggc ttattaggtg gcacatatat agatgtatct
     3181 gcctcatcag ctggcaaagc agatgacaca ggatttaccc atacattttc ttatggtgct
     3241 ggtgctgatt ttgccatcaa aaatggtatt cgtttaggtg ccgattttat tcaatacatg
     3301 gatgactaca acgctattag tgtgggtatt cgtattccca ttcaataatc atgtacgcct
     3361 cgctcttagt agattaaggg cgaggcttca ttatatctgt gttactgttt cacatcttgt
     3421 tagcttgtct gtgtttttat atcccatcaa taaaaaactt taacgtttac ccatagaacg
     3481 acgtgtgcca tgtggcggtc tgctgggacg ttggctaaaa cgatctttat ttggctgtgg
     3541 ataattagtc gcttcttttt tgctttgcaa agattcagcc aataacgctg ttaaggtttt
     3601 ggtggtttga ggactttgct ctgcggagat attttctgag gtattcataa tttactgcta
     3661 aataaaaaca taattgagtc agattaggac ttacgcggtt atcgcttatt tattcgcatt
     3721 taaaccattt ttaacggttt tatgctcaca aaacatgcgt acgtcatgaa ttatacgcta
     3781 cccatcttag gagctgttga ccttataagc agcaaaacaa aaaaaagcgt actgttttgc
     3841 agtacgcttt tccaaaaaca gactagcatt atttttttga aacgatgcta gtcgtcatgc
     3901 gacttaatta gttgcgagtt tggtcaacta atttgttttt gtgaatccaa ggcatcatcg
     3961 cacgtaattg agcaccaact ttctcaattg ggtgatcagc atttaaacga cgacgagcgg
     4021 tcatttctgg gtagtttgta cgaccttcta aaatgaagcg ttttgcgtat tcaccgtttt
     4081 ggatgttacg taaacattca cgcatagctt cgcgggcttg agtgttaata acacgttggc
     4141 cagtcacgta ctcaccgtat tcagcattgt tagaaataga gtagttcatg ttagcgatac
     4201 cgccttcata catgaggtct acaatcaatt tcaattcgtg taaacattca aaataagcca
     4261 tttctggagc ataacctgct tcagtcaatg tttcgaagcc catttttacc aattcaactg
     4321 cgccaccaca caatactgct tgttcaccaa acaagtcagt ttcggtttct tcgcggaagt
     4381 tagtttcaat cacaccacct tttgtaccac cattagcagc cgcataagat aaagcgattt
     4441 gtttagagtt acctgatttg tcttggtaaa cagcgattaa gcttggtaca ccgccacctt
     4501 ttaagtattc agaacgtaca gtgtggccag gaccttttgg agctaccata ataacgtcta
     4561 agtcagcacg tggtacaact tggttgtaat gtacgttaaa gccatgagca aacgctaaag
     4621 ttgcgccttg cttgatgttt ggctcgatgt cgttacggta aacttctgga atgttttcgt
     4681 ctggtaacaa aatcatcaca acgtctgcgc tttttacagc ttcagcaact tcggcaactt
     4741 taatgccagc tttttcaact ttagcccagc ttgcgccgcc tttacgtaaa ccaacagtca
     4801 cgtcaacacc agaatctgtt aagttttgtg cgtgagcatg gccttgagaa ccgtaaccaa
     4861 cgatagtcac ttttttgcca cggatgatag ataaatcggc atctttatcg taataaactt
     4921 gcatgagaat tcctcaacaa aaactaagaa agcttgctgc tttctgattg gggtgaatat
     4981 attccctccc ctaatttagg agagggtcag ggtggggtct aaaatccccc ttcccaacct
     5041 cccccttaat aagagggagg agtaacatta aatacttaac actttctcgc cacgagcaat
     5101 accagacacg ccactgcgta ccacttctaa aattgtggtt tcagagagtg cttcaataaa
     5161 ggcatctaat ttgtcagatg tacctgccaa ttgaatggta taagtgcttg aagtaacatc
     5221 aacaatttga ccacggaaaa tatcagcagt acgtttgatc tcggcacgaa tcgcgcccat
     5281 ggctttgact ttaatgagca ttaactcacg ctcaatatgg tcaccttcgc ttaaatcaac
     5341 cactttcacc acttcaacta atttattgag ttgtttggtg acttgctcaa ttttatggtc
     5401 atcaccagaa gtggttaagg ttaaacgcga taaagtagca tcgtcagttg gtgcaacagt
     5461 taaggtttcg atgttgtaac cacgttgact aaacaaaccg actaaacgtg acaatgcgcc
     5521 cgcttcgttt tcaattaata cagaaataat atggcnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnactaa acgtgacaat gcgcccgctt cgttttcaat taatacagaa
     5701 ataatatggc gcatgattaa gtacgctccg ttttcgacaa ccacatatca cgcatagatt
     5761 gattagcaat caacatcgga taaacgtgtt caaattggtc aacataaaca tccataaaca
     5821 ctaaacgatc tttcatggca aacgcttcac gcattttgct ttctaattcg ttaggatcgg
     5881 taattttcat gccaacatga ccataagctt caactaattt aacgaagtca ggtaaagact
     5941 ccatataaga attagaatga cggccttcgt attgcatatc ttgccattgc ttaaccatac
     6001 ccaaagcacg gttgttcaag tttaaaatct tgacaggcaa gttgtactgc aaacaggttg
     6061 acaattcttg gatattcatt tgaattgagg cttcgccagt aatacaaacg actggttggt
     6121 ctgggaatgc catcaaacag cccatggctg ctggcaaacc aaagcccatc gtgcctaaac
     6181 caccagagtt aatccattga cgtggacgat cgtatttgta atacaacgca gcaaacattt
     6241 gatgttgacc aacgtcagag gtcacaatgg ctttgccttc agtgactttg tataacatct
     6301 caaccacttc ttgtggcttc atttcgcctg ctttggctgg ctcataacgt aaaccatgat
     6361 gtgcgcgcca tgcgtcaatt tgtttccacc aagcggcaat cgcttctgga tttggcttgt
     6421 tgacacccat ttgttttaac tgagctaaca tttcggttaa aacaggctct actgcaccca
     6481 caatcggaat atgtgccgta atcgtcttag aaatagtcgc tgggtcaata tcaatatgaa
     6541 tgacttttgc ttgagtacag aattttttcg ggttattggt cacacggtca tcaaaacgcg
     6601 ccccaattgc caagatcaaa tctgcatggt gcatggtcat gttggcttca taagtaccat
     6661 gcatacccaa cataccaacg aactgacgat cagaacctgg ataagcacct aagcccatca
     6721 aagtattagt cactgggtag tttaacaact gcgctaattc aaccaataat gctgaggcat
     6781 taccttgaac aacaccgcca cccgtataaa taatggggcg ttttgcttga attaattcat
     6841 caactgcttt tttgatttgt ccagcatgac cacgtgatgg tggattgtaa gaacgcaatt
     6901 ttaccttttc gggatattca taagggaatt tctcgttagg tgcagtgcag ctcttaggaa
     6961 tatcaataac cacagggcct ggacgaccag aagaggctaa ataaaaagcc ttcttaacca
     7021 ctgctggaat ttctgaagca tgacgaattt gaaagctatg ctttacgata ggacgagaaa
     7081 caccaatcat atcggtttct tggaacgcat cttcaccaat caaatgtgaa ggaacttgac
     7141 ccgaaataac taccatcggg atcgaatcca tataagccgt tgcaattggc gttaccgtat
     7201 tggttgcacc aggtccagag gtgactaata ccacacctgt tttgcctgtt gaacgagaat
     7261 aaccgtcagc catgtgccct gcggcttgct catgacgcac taaaatatgc tgtacacggt
     7321 cttgttgaaa aatcgcatca taaatatgga gtaccgcacc accgggatat ccaaacacaa
     7381 actccactcc ctcatctgcg agggcgcgga ttaacatttc accaccagat aaaagttcca
     7441 cgactatcac cctttcaaga tatagcatta atttgagtct ataacagcta aactagccat
     7501 tttctcaata tctgctattt aagacagatt caattttcag gaaccgcact gtgatcctga
     7561 tttttgcaac ttaggaaaaa cccaagccca cctaaaaatt atatcagcgg tttaaacaag
     7621 actctgggca ttcgtattat ggtcaatacg tttatggtta aggacaggtt ttgtcactca
     7681 acgatattca tcaagtctat attccaaaac tacgttcagt agattttgga cattatcatt
     7741 aacaatactg acaagagggt gacttgttca gtattgtttc cgctaatttc tcttaaaact
     7801 tattttattc aataagctcg tcttgctaat gcatgaataa acgtacaaag aaggtttatt
     7861 ataagctagt cttacttagc aagcattaca caaaacataa aagggtaagc atttttatag
     7921 ttacccctct ttatgtcaag accaaaatga ctttttttgt ttgtttgtta acactctata
     7981 gatttgatga cattgtttaa cttatcaaac acaaaaaaaa cagctatagt caccaacatt
     8041 aaattattcg cccatgatgg gagtaaatgg ccaatgaaca aaaaacaaaa aatgatttct
     8101 atactaagca ttttcacaag tgctgtacta tctgcttcta ttgtttctgc tgatgaagat
     8161 ggagctatat acaaatgggt agatgagcgt ggcgtgacta attattccga aacgccacct
     8221 gatgctaaag gtgcaaatgc taaagcacaa tccattaaca tcaatcgtta tgttccgcgt
     8281 ggcagtgaaa atgcagtcaa acaattagaa caacaacgca cagaaaaagn nnnnnnnnnn
     8341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tattactcac ccaccaacca acgaacactc
     8461 gctttagcta atttattttg acttaattgt ggcaacaacc attgcaacac tttgtctgct
     8521 tcttcggcaa ataaatatgg cggattaacg attaataaac cacaaccatt caagcccaaa
     8581 ttgttatcat caggcatgac acacagctca caaactaatt gtttgcgaat acccgtttta
     8641 actaaacgac gctcaaaacg ctcaatcgac gcacgatctt tgatgggata ccaaatggca
     8701 tacatacctg ttggccattt gtcataagct ttcgctaata actcaaccac ttgtgggtaa
     8761 tcatgacgtt cttcttcata aggtgggtca attaacacca aaccacgctt ttctttgggt
     8821 ggaatgagcg catttaagcc ttcatacgca tcacgatgat ggacagcaac ttgtggatga
     8881 cggtaaaaat tatcttttaa cttttcaacg tcaacaggat gccattccat taacaccatg
     8941 cgatcttgtt cccgaatatt ttggatggca aaccacggtg aaccaccata ataggctttg
     9001 ctaccaaaat gtgttcttaa agtttcaaaa ttttctaaat aggtttcgac ccatttgcta
     9061 ggtttggctt ggcgggtatc aattttacga atgccttcta agtattcgcc tgttttttgg
     9121 gctggagcag ccgctaaatc gtaccaaccg cgtccagcat gggtatcaat ataacaaaaa
     9181 gcattgtcct tcttatgcaa ggcttgtaat aatccaattt ctaagatgtg tttcatcaca
     9241 tcggcgaaat ttcctgcgtg gtaatggtgt cggtaattca tgcaaagttc tcaaaatcaa
     9301 caatttaata aatcagtatt caatcttaat caggcattcg acgctttagc gactgatcat
     9361 tatgccgtga ttccacaatt cttaacgtca acaatgcgtg aggctttatt agccgaattg
     9421 ttacagttgc aagcgcaagg tttatttcat gaagcgagtg ttggtcgagg cgttggacaa
     9481 actcgtcaag ctcatattcg cggcgatagc atttgttggt tggaagatca ttttactata
     9541 ggtggacagt atctggcact gatggaacag cttcggcaac aattaaatgc cgatttttat
     9601 ttgggtttac gctcttatga ggcacattat gcccattatc aggctggcag tgtttatcaa
     9661 cgccacgttg atcggcatcg ggataataat gcacgggtgg tgacaacggt atgttatttg
     9721 aatgatgact ggccaactga ggcaggagga cagctaaaac tttataaccc aaaccacgaa
     9781 ttattgcttg agcaaacgcc aacaggtggc agtttagtgt tatttatgag tgctgatatg
     9841 ccgcacgagg ttttagcggc aaatcgctca agatttagta ttgcaggttg gtttagacag
     9901 gattagccat ctggggaaag atttttcc
//
LOCUS       DAOQMB010000043         9890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2667_length_9890_cov_15.269548, whole genome shotgun sequence.
ACCESSION   DAOQMB010000043 DAOQMB010000000
VERSION     DAOQMB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2667_length_9890_cov_15.269548"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="PLY05_02940"
     CDS             <1..2231
                     /locus_tag="PLY05_02940"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNC02288.1"
                     /translation="LGPLSSAEANMQQVASLGGGKPYKATDTAALVKAFTDVVDESIK
                     SSGTISAAGVAVNQLNRLNHLDQLYYAVFDPKPNSLHWEGNLKRYRLGTDGVIYDNST
                     PAKNAVNSDTGFFKSDAKSFWSTEVDGENASAGGAASKLTATKLPTRKMYTYTDALTT
                     LSSPVSLQEIDFTNLGSTTTLNTTLQGKTGTSGTEYINLMKWYQGYDVDSLTSTDIPT
                     EARQRLGGALHSRPILVNYGYTGELATANNADNQDNYLFFSTLEGSLHVVNAKTGEEK
                     FTFTPGEKLATLKDLYLNPAAALPAFGMDLTWVSYRKDTDGSGQIGTGDKVWLYGGMR
                     MGGDNYYALDVTNLDSPKLMFAIDGGKTGTKYARMGQTWSEPVITSIYVEGKPKRVMI
                     FGGGYDDKHENHALALPFTDIDKGNQIYIVDAEKGDLLWMASGNSTDSPDKLVDDMKF
                     SIPSSPKAVDLDSDGVADVIYIGDLGGQVFRIDLDRKATTKTNIAKRVRLIAKVGQTE
                     TANLVSQRRFYEPPAVATFKNTSGSLFAAVSMGSGYRSRPLNTVTDERFYTFFDYDVT
                     KPNLLSLEDSALQAVITHADLDLLDMNAATTQQNGVGQTKKGWYVDFPESGEKALASG
                     FIFKEKLVFSSYSPTVSSTTNCTPVKGKTNLYTMCMPYGKMCWAAGSEPTGFNNYKKS
                     NIMAGLGGEPQLILTKNPSTGANELNVLVGTSVDKGIFAGTTAGGAQLVPTKKWREKT
                     KK"
     gene            2305..2760
                     /locus_tag="PLY05_02945"
     CDS             2305..2760
                     /locus_tag="PLY05_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016806925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNC02289.1"
                     /translation="MSKQNKGFTLMELMVTVAIIGILASIAIPSYRQYVLRGNRADMQ
                     AQMLVIAAALERYKAQNFRYQGATLSNTGIYGASVYPQQGQAHYTLTLTGDNTDGTPN
                     LTWTLKAQATSNTQKQDGVLMLNSAGQRCWLQGAATTTCTLGDAAQGWN"
     gene            complement(2757..3230)
                     /locus_tag="PLY05_02950"
     CDS             complement(2757..3230)
                     /locus_tag="PLY05_02950"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HNC02290.1"
                     /translation="MATFRKTQQAQLISKELQHIVHSARGLALTKRRALTLCGSTDGY
                     SCDQNWSQGALLFEDTNRDGQFDSDEFLVKYTPFALKNSQLIWKGFLGSFMRFESMGI
                     TSASNGTFTYCQTDHDPRFSRQVIVSRGGRARLSQDKNGDGIHEDISGAAKINCP"
     gene            3386..>3816
                     /locus_tag="PLY05_02955"
     CDS             3386..>3816
                     /locus_tag="PLY05_02955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC02291.1"
                     /translation="MNIVVIGSGVIGLLQAYKLLKLGHQVTILEKNQQEFESSWAGGG
                     IVSPMYPWRYSAAVTALAQLAQAQYPLLAKELFELTGIDVELIPCGMLMLETEDRRDA
                     LAWGQQNQQTVFELGQSQIMEINPNLASFKQAIWLPHIANIR"
     assembly_gap    3817..3916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3917..5316)
                     /locus_tag="PLY05_02960"
     CDS             complement(<3917..5316)
                     /locus_tag="PLY05_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004656362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC02292.1"
                     /translation="MTTKALIIDDEPDLTELLAITLERMGIECRLANDVTTALNLLKK
                     ETFQLCLTDMHLPDGSGLDIVQTIQKLYPQTPVAVITAYGSTEMAVTVLKAGAFDFVS
                     KPIELPRLRDLVRNALKVSGQHRPKDDYENRLLGQSQVMLDLKETIRKLARSQAPVYI
                     SGESGTGKEVVARLIHDLSPRAEKPFIPVNCGAIPSELMESEFFGHKKGSFTGATEDK
                     IGLFQAANEGTLFLDEVADLPISMQVKLLRAIQEKTVRPIGHAKEIPVNIRLLSATHK
                     DLSAEVAAGRFRQDLFYRINVIQARIPPLKERGEDIILLANNFLHRLSEDWGITEPKL
                     SPSARHALKHYPFPGNVRELQNILERALTLCEGNEILAEHLQLPTHTPFSTSNKTPTP
                     AHGVHAYSDNTPPPSNSESLEDYLLTIEKNLIIKALDDTRWNRTAAAKKLGMTFRSLR
                     YRLKKLGLDDDSDDDN"
     gene            complement(5318..>6685)
                     /locus_tag="PLY05_02965"
     CDS             complement(5318..>6685)
                     /locus_tag="PLY05_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02293.1"
                     /translation="LQAFFFVLIDIIFITLIMHANGGPTAQLSLLYLVMVAAGNILLP
                     DKRGPLIAAVASIAVLYEQFYFTLNNYERISSANLVQASILGISFFAIALFSQLISHR
                     FRQVEALALQKSLEVESLQQLNEQIINRMHTGVLVVNPERELLLYNNAAQQLLGINDI
                     HKTDHLKNISGTIDAGLLAWRQNLMIRPLVFKNQATYPEINVSYMQLDIQNKESNILI
                     FLENTAQMTQKAQQLKLASLGRLTASIAHEIRNPLGAISHAAQLLIESEDIKGPDARL
                     LKIIEQHCVRVNNIVETVLQLSRRQKSTPQLLNLKVWLDNFIDEYHQTNHQQKTIIFQ
                     STDNILIRFDTDQLYQVIYNMVNNAFRYSAKNGYHTIELMADVDDLTQLPYLEIYDLG
                     PGVALENQKNLFEPFFTTEKNGTGLGLYLSKELCEANQAHLDYLPQPEGACFRITFSH
                     PNRLI"
     assembly_gap    6686..6785
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6786..6994)
                     /locus_tag="PLY05_02970"
     CDS             complement(<6786..6994)
                     /locus_tag="PLY05_02970"
                     /inference="COORDINATES: protein motif:HMM:NF041023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HNC02294.1"
                     /translation="MGGILRLLIIAAVVYLAYVWIRKLLNSQQHTTEVQKADEPRQLM
                     KQCAFCQVHIPEGESTQSQGHFFCS"
     gene            7157..8767
                     /locus_tag="PLY05_02975"
     CDS             7157..8767
                     /locus_tag="PLY05_02975"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNC02295.1"
                     /translation="MTLRIAFAQHNFVVGAITENSHKVRQLAEQAKQQGAEMIVFPEL
                     CLTGYPPEDLLLRPSLTIRIEQALQSLADIKNIVLVLAYPKMIAGQLFNMAGVWLNGQ
                     CLGEYAKQKLPNYQVFDEKRYFSAGTQGFYFDYKGQKIALAICEDIWHDEVAIQAKNA
                     KTDLLVVLNASPFHINKQAQRLQMVQQRAQQHQLPIAYVNLVGGQDELVFDGGSFMIN
                     ADGDIAAEAPLFVEELLLVDFDKQFSKKERAKLLSEEAAVYQALVLGVKDYVSKSGFK
                     GVVLGLSGGIDSALVLAIAVDALGAEQVNAVMMPYHYTSQMSLEDAAQEAKTLGVNYS
                     IIEIAPTVEAFSQLLAPAFAGMAKDKTEENLQARSRGVLLMALSNKKGWLVLTTGNKS
                     EMAVGYATLYGDMAGGFDVLKDVPKTLVFRLAEYRNRLANQEIIPQRVIDRPPSAELA
                     PDQKDEDSLPPYSVLDAILALYIEHDQSAEAIINQGFERDTVYRVLRMVDFNEYKRRQ
                     AAIGPRISQRGFGRDRRYPLVNGWKIGI"
     gene            complement(8807..9439)
                     /locus_tag="PLY05_02980"
     CDS             complement(8807..9439)
                     /locus_tag="PLY05_02980"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HNC02296.1"
                     /translation="MSKKSKKPRQSHTTTPTPPEATQVELAGIGVRLAAVIYDGLLIF
                     AINAIVAVILVVIATPSEVSSQNQTQVLSSNFRHLVLFPAMVVMTWLFYGYFWRRRGQ
                     TLGMQTWRLKVIKPDGRLLNWSDSMGRCAASLILPSICGLAANVLYHSEGAFALSLVF
                     GFVGNYLWALINGRRLAWHDQLSATVVIRVPEDPRQKRGMLGWFSKTDEE"
     gene            9671..9883
                     /gene="rpmE"
                     /locus_tag="PLY05_02985"
     CDS             9671..9883
                     /gene="rpmE"
                     /locus_tag="PLY05_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNC02297.1"
                     /translation="MKADIHPKYEEITATCSCGNVIVTRSTLAKNIHLDVCSKCHPFY
                     TGQQKVVDTGGRIDKFKQRFNRFAKK"
BASE COUNT         2968 a         1679 c         2049 g         2994 t
ORIGIN      
        1 ttttaggtcc gttgtctagt gcagaagcga atatgcagca agtagcgtct ttaggcggtg
       61 gaaaacctta taaagcaaca gatacggctg ctttagtaaa agcttttact gatgttgttg
      121 atgagtcaat caagtctagt ggtacgatta gtgcagcagg tgtggcagtt aaccagttaa
      181 accgtttaaa tcatttagac cagctttatt atgccgtgtt tgaccctaag cctaattctt
      241 tacattggga gggtaactta aaacgttatc gtttggggac cgatggtgta atttacgata
      301 actccacgcc agccaaaaat gcggttaatt cagatactgg attttttaaa agtgatgcta
      361 aaagtttttg gagtactgag gttgatgggg aaaatgcctc tgcaggtggt gcagcaagca
      421 aattaacagc cacaaaatta cccaccagaa aaatgtacac ctatacagat gcattgacta
      481 cactgtcctc accagttagc ttgcaagaaa ttgattttac taatttgggc agcacaacga
      541 ctctaaatac gacgcttcaa ggaaaaacgg gtacatcagg gacagagtat attaatttaa
      601 tgaaatggta tcagggatat gatgtagatt ctttgacgtc cactgatatt cctacagaag
      661 ctcgtcagcg tttagggggg gcattacatt cgcgtcctat tttggttaat tatggttata
      721 ctggtgagtt agctacggct aataatgcag ataatcaaga taactatttg ttttttagta
      781 ccttagaggg atcgttacat gttgttaatg caaaaacagg tgaagaaaag tttacgttca
      841 cgcctggtga gaaattagca actttaaaag atttgtattt aaatcctgct gcagccttgc
      901 cagcttttgg tatggactta acatgggtat cttatagaaa agataccgat ggttctggac
      961 aaattggtac aggtgataaa gtctggttat atggtggtat gcgtatgggg ggggataact
     1021 attacgcctt ggacgtgacg aatctagata gtcctaaatt aatgtttgcg attgatggag
     1081 gtaaaacagg tactaaatat gccagaatgg ggcaaacatg gtcggaacct gttattacca
     1141 gtatttatgt agagggtaag ccaaaaagag tgatgatttt tggtggtggt tacgatgata
     1201 agcatgagaa ccatgctttg gcgttacctt ttacagatat agataaaggt aatcaaattt
     1261 atattgttga tgccgaaaaa ggtgacttat tgtggatggc tagtggcaat agcaccgatt
     1321 ctccagataa actcgttgat gatatgaaat ttagtattcc ttcgtcacct aaagccgtcg
     1381 atttagattc tgatggagtg gcagacgtta tttatattgg cgatttaggc ggacaagtat
     1441 ttagaataga tttagatcgt aaagcaacga ccaaaacaaa tatagcaaag cgtgtaagac
     1501 taattgcaaa agtaggccaa acagaaacag ctaatttagt cagtcaacgc cgtttttatg
     1561 agcctcctgc cgttgctact tttaaaaata cctcaggcag tctttttgct gctgttagta
     1621 tggggagcgg atatagaagc cgaccattaa ataccgtgac tgatgagcgt ttctatactt
     1681 tctttgatta tgatgtcacc aagcctaatt tattgagttt ggaggattca gccttgcaag
     1741 cagttattac tcatgctgat ttggatttat tagatatgaa tgcagcaaca actcaacaaa
     1801 atggtgtggg acaaaccaaa aaaggttggt atgttgactt tcctgaatct ggtgaaaagg
     1861 ctttggcatc aggatttata tttaaggaga agttggtttt tagttcttat tcaccaacag
     1921 tgtcttcaac cactaactgt acgccagtta aaggaaaaac aaatctctat actatgtgta
     1981 tgccttatgg aaaaatgtgt tgggcagctg gttcagagcc tacaggtttt aataattata
     2041 aaaagagtaa tattatggcg ggcttggggg gcgagcctca gttgattctc acaaaaaatc
     2101 cgtcaacagg tgctaatgag ctaaatgtgt tggtaggtac ttcggttgat aaaggtattt
     2161 ttgcaggaac aacggctggc ggcgcacaac tagtaccaac caaaaaatgg cgtgagaaaa
     2221 caaaaaaatg atgttggttt taggttggat ttcagattcg gcctaactct aaataataga
     2281 ataggatgtg gggaggtgtg tataatgagt aagcaaaata agggctttac ccttatggag
     2341 ttgatggtaa cagtggcaat tattggtatt ttagcctcta tagcgattcc tagttatcgg
     2401 caatatgttt tacgaggtaa tcgtgctgat atgcaggcac agatgttagt gattgcagct
     2461 gcactagagc gatataaggc gcaaaatttt agatatcagg gggcaactct tagtaatacg
     2521 ggtatttatg gggcctctgt ttatcctcag caagggcaag ctcactatac attaacattg
     2581 actggtgaca atactgatgg aacgcccaat ttaacatgga cactaaaagc tcaagcaaca
     2641 agtaatactc aaaaacaaga tggggttctt atgttgaaca gtgcaggtca acgttgttgg
     2701 ttacaaggtg ctgcaacgac aacatgtact ttaggtgatg cagctcaagg atggaattaa
     2761 gggcagttta ttttggccgc acctgatata tcttcatgta tgccatcgcc atttttatcc
     2821 tgtgatagtc tagcacgtcc cccacgactg actatcactt gtcggctaaa acggggatca
     2881 tggtcagttt ggcagtaagt aaacgtgcca ttgctggcag atgtgattcc catactctca
     2941 aagcgcataa atgaccccag aaaacctttc caaatgagtt gcgagttttt taaggcaaac
     3001 ggggtatatt ttactaaaaa ttcatcactg tcaaattggc catctctatt ggtatcttca
     3061 aacagtaaag caccttgact ccaattttga tcacaactgt agccgtctgt tgatccgcac
     3121 aaagttaatg ctctgcgttt ggttaaggct aatccgcgag ctgaatgaac aatatgttgt
     3181 agttctttgc taatcagttg agcttgttgt gttttacgaa atgttgccat gttgtgtgct
     3241 gcaaatagca gtataatcat aaaaattaat acacaggcaa tagtctcaag taatgtaaat
     3301 gctctttgaa agtacatatt tatatcctta taaattttta atcctatctt tttatattag
     3361 gttttttgtg tgttggagtc aatatatgaa tattgttgtg ataggttctg gagtcattgg
     3421 cttattacaa gcatataaat tattaaaatt aggacatcaa gtcaccattt tagaaaaaaa
     3481 tcagcaagag tttgagtctt cttgggctgg cggagggatt gtttcaccca tgtatccttg
     3541 gcgatattct gctgccgtta cagctttggc acaattggct caagcacaat atccgctttt
     3601 agctaaagaa ctatttgaat taacgggtat tgatgttgaa ttaatacctt gtggtatgtt
     3661 gatgttagaa acggaagata gacgagatgc tttagcttgg ggacaacaaa atcaacaaac
     3721 ggtttttgaa ttagggcaaa gtcaaattat ggagattaac cctaatttag ccagttttaa
     3781 acaagctatt tggttgccac atattgcgaa tatacgnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnntcat tatcatcatc gctatcgtca tctaaaccta atttctttaa
     3961 acggtagcgt aatgaacgaa aagtcatacc taattttttc gctgcagcag tacgattcca
     4021 acgtgtatca tctaaggctt taataattaa gttcttttca atggttaata aataatcttc
     4081 aagagattcg ctattacttg gtggaggtgt gttgtcacta taagcatgta caccatgagc
     4141 gggggtggga gttttattgc tagtactaaa aggcgtatgt gttggcagtt gtagatgctc
     4201 tgctaaaatt tcattgcctt cgcataacgt taaagcacgc tctaaaatat tttgcagctc
     4261 tctcacattt cctggaaagg gataatgctt taaagcatgc cgtgctgatg gactcaattt
     4321 tggctcagta atgccccagt cttcgctaag ccgatgtaaa aagttattgg ctagcaaaat
     4381 aatgtcttca ccacgctctt tgagtggtgg gatgcgcgct tgaataacat tgattcgata
     4441 aaataaatct tgacgaaatc gtccagcagc tacttcagca gacaagtctt tgtgtgtggc
     4501 acttaacaaa cgaatattga caggaatttc tttcgcatgg ccaatcgggc gaacagtctt
     4561 ttcttggatg gcacgtaaca gcttaacctg catactaata ggcaagtctg caacctcatc
     4621 taaaaataaa gttccttcat tggcggcttg aaataggcca attttgtctt ctgtcgcgcc
     4681 tgtaaagcta ccttttttat ggccaaaaaa ttcactttcc atcagttctg aaggaatcgc
     4741 accacagtta acaggaataa agggtttttc ggctcgtggg cttaaatcat gaattagacg
     4801 ggcaacaact tctttaccag taccagattc gccactgata taaacaggtg cttggctacg
     4861 agctaattta cgaatcgttt cttttaagtc aagcatgact tgtgattggc ctaaaagtct
     4921 attttcatag tcatctttgg ggcgatgttg accagatact tttagggcat tacgcactaa
     4981 atcgcgaagg cggggtagtt caataggttt ggaaacaaaa tcaaacgcac ccgcttttag
     5041 cacagtgacc gccatctcgg tactgccata agcagtaatg actgcaacag gtgtttgcgg
     5101 ataaagtttt tgaattgttt gcacgatatc taaaccacta ccatcaggta agtgcatatc
     5161 ggttaaacaa agttgaaaag tttctttttt tagtaaattt aaagcggtag tcacatcatt
     5221 tgctaaacga cattctatcc ccattcgctc aagcgtgatg gctaaaagtt cagttaagtc
     5281 tggttcatca tcaatgatta atgcttttgt tgtcatatta aattagccga ttaggatgag
     5341 aaaaagtaat tctaaaacaa gcaccttcgg gctgaggtaa gtaatctaaa tgagcttgat
     5401 tggcttcaca aagctctttt gataaatata agcctaaacc cgtaccattt ttttcagtag
     5461 taaaaaatgg ttcaaataaa tttttttggt tttcaagcgc aacgcctggc cctaaatcat
     5521 aaatttctaa atacggtagt tgcgttaaat cgtcaacatc agccataagt tcaatcgtat
     5581 gatagccatt tttggcacta taacgaaaag cgttatttac catattataa atgacttgat
     5641 acagctgatc ggtatcaaaa cgaatcagta tattatcggt tgactgaaat atgatcgttt
     5701 tttgttgatg attggtttgg tgatattcat cgataaaatt atcaagccat acttttagat
     5761 ttaatagttg tggtgttgat ttttgtcggc gtgataactg caaaactgtt tcaactatat
     5821 tattaacacg aacgcagtgt tgttcaataa tttttaataa tcgagcgtct ggtcctttaa
     5881 tatcttcaga ctctattaat aattgggctg catgactgat tgcccctaaa ggattacgaa
     5941 tttcgtgggc aatactagcg gttaaacgac ctaatgatgc taattttaat tgttgggctt
     6001 tttgggtcat ttgagctgta ttttctaaaa aaataagtat attactttct ttgttttgaa
     6061 tgtctaactg catatatgaa acattgattt ctgggtaagt ggcttggttt ttaaagacta
     6121 gaggtcgaat cattaaattt tgtcgccatg ctaataaacc agcatcaata gtgcctgaaa
     6181 tgttttttaa atgatcggtt ttatgaatat cattaatgcc taataattgt tgagcggcat
     6241 tattatatag tagtagctct cgttcaggat ttaccactaa tacacctgtg tgcattctat
     6301 tgataatttg ttcattgagt tgttgtaaac tttctacctc taaggatttt tgtagcgcta
     6361 aggcttcaac ttgtcgaaaa cgatgactaa ttaattgact aaataaagca attgcaaaaa
     6421 aactaatccc taaaatagag gcttgcacta aattagcgga gctgatgcgt tcataattat
     6481 ttaaggtaaa ataaaattgc tcatataaaa cagcaatgga ggcaacggct gcaatcagtg
     6541 ggccgcgttt atcaggtaat aaaatattac ctgctgccac catgacgaga taaagtaaac
     6601 taagttgtgc ggtcggccca ccatttgcgt gcatgatgag cgtgataaaa ataatatcaa
     6661 ttaaaacaaa aaagaatgcc tgtaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6781 nnnnntcact gcaaaaaaaa tgtccttgag attgtgtgct ttctccctcg ggaatatgga
     6841 cctgacaaaa agcgcactgt ttcattaatt gtcgaggctc gtcagctttt tgtacctctg
     6901 tagtatgctg ttgtgaattg agtaatttac gaatccacac ataagctaaa taaacaacgg
     6961 cagcaataat taataaacgc agaataccac ccataaacac ctcaacgttt ttaaaattga
     7021 cgcggttatc gcttatttgc gtgctaaata atcgttttta acgattttta gcgcgcaaat
     7081 aagcgctaat ttcagtgaaa tgcgccagtc ctgttttata atctatacca atttgaattt
     7141 tggtatagag aaagttatga ctctacgtat tgcttttgct caacataatt ttgtcgttgg
     7201 tgctattact gaaaacagcc acaaagttcg tcaacttgct gagcaagcta aacaacaagg
     7261 cgcggaaatg atagtttttc ctgaattgtg tttaacaggc tatcctccag aagatttatt
     7321 gttacgccct agtttaacga ttcgtattga acaagccttg cagagtctag cagacattaa
     7381 aaatattgtc cttgttttag cttatccaaa aatgatagca ggccaattat ttaatatggc
     7441 aggtgtgtgg ttaaatggtc aatgtttggg agagtatgcc aaacaaaaat tacctaatta
     7501 tcaagtgttt gatgaaaaaa gatattttag tgcaggtaca caaggatttt atttcgatta
     7561 taaaggtcaa aaaattgcgt tagcgatttg tgaagatatt tggcacgatg aggttgcaat
     7621 acaggccaaa aatgcaaaaa cggatttatt agtggtgtta aatgcctcgc cctttcatat
     7681 taataagcaa gcgcagcgtt tgcagatggt tcaacaacgt gcccaacaac atcaattgcc
     7741 aattgcttat gttaatttag tgggtgggca ggatgagtta gtctttgatg gtggctcttt
     7801 tatgattaat gcagatggtg atatagctgc tgaagcacca ttatttgtag aagagttatt
     7861 actagttgat tttgataaac aatttagtaa aaaagaacga gctaaattat taagtgaaga
     7921 agctgctgtt tatcaagctc ttgtgcttgg tgtaaaggat tatgtgagta aaagtggttt
     7981 taaaggagtg gtgttaggtt tgtcgggcgg gattgattca gctttagtgt tggcaatagc
     8041 ggttgatgcc ttgggggcag agcaggttaa tgcggtgatg atgccttatc attatacttc
     8101 tcaaatgagc ttggaagatg ccgcacaaga agcaaagact ttaggtgtga actattcaat
     8161 cattgaaatt gctccgacag tagaggcatt ttcccaatta ttagcaccag cttttgcagg
     8221 catggctaaa gataaaactg aagaaaattt acaagcccgt tcgcgtggtg tgctgttaat
     8281 ggctttatca aacaaaaagg gctggttagt attaaccaca ggcaataaat cagaaatggc
     8341 tgtgggctat gcgacattgt atggtgatat ggcaggtggt tttgatgtgc taaaagatgt
     8401 ccccaaaacc ttagtcttta gattagcaga atatcgaaat cgtttggcaa atcaagaaat
     8461 tattcctcag cgtgtgatag atcgcccacc ttctgcagaa ttagcacctg atcaaaaaga
     8521 tgaggatagt ttgccgccat attccgtttt agatgctatt ttagctttat atattgaaca
     8581 tgatcaaagt gctgaagcga ttatcaatca aggttttgag agagatactg tatatcgtgt
     8641 gttaagaatg gttgatttta atgagtataa acgtcgtcag gcagccattg gccctcgtat
     8701 tagtcagcgt ggttttggtc gagatagacg ttatccttta gttaatggct ggaaaattgg
     8761 catttgagtt tacataagag tagcgaagct ctcaataata ttttcgctac tcttcatcgg
     8821 ttttagagaa ccatcccaac ataccgcgtt tttggcgtgg gtcttcaggc acacgaatga
     8881 caacagttgc agagagttga tcatgccaag caaggcgtct accattaatc aatgcccaaa
     8941 gataattacc aacaaagcca aaaactagac tcagagcaaa tgcaccctca ctatgataaa
     9001 gaacatttgc cgctaaacca caaatacttg gcaaaattaa actcgcggca caacgcccca
     9061 tactgtctga ccaatttaat aatcgaccat caggtttaat gacttttaaa cgccatgttt
     9121 gcatacccaa agtttgaccg cgtctgcgcc agaaatagcc ataaaatagc caagtcatta
     9181 caaccattgc tggaaataat actaaatggc gaaaattgct tgataaaact tgagtttgat
     9241 tttggctcga aacctcagaa ggcgtagcaa tcacaactaa aataactgca acaatcgcat
     9301 taatcgcaaa aattaataac ccatcataaa tgacagcagc taagcgaacg ccaatacccg
     9361 ctaactcaac ttgggttgct tcaggaggtg ttggtgtcgt agtgtggctt tggcgtggtt
     9421 ttttggattt tttagacatt ggtacagact atattcacaa gactagaatt agcattatat
     9481 cgtgaataga gaaaaaataa gtgcagagat ggttgaaatt tgttatattt cgcgcccttg
     9541 ttgcgctgag ctctaacgaa gtgactgtta gggttagcca aaagaatggt cgttgttcgt
     9601 tcctgctcca agccgatgag ccttacgcag gggggcgagc actccttaaa ccactctttg
     9661 agatttaatc atgaaagctg atatccatcc aaaatatgaa gaaattactg caacttgttc
     9721 ttgtggtaat gttattgtga ctcgctctac cttagctaaa aacattcacc ttgacgtttg
     9781 tagcaagtgt cacccattct acactggtca acaaaaagtt gttgatactg gtggccgtat
     9841 cgacaaattt aaacaacgct ttaatcgttt tgctaaaaaa taattttagt
//
LOCUS       DAOQMB010000044         9805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2700_length_9805_cov_15.063385, whole genome shotgun sequence.
ACCESSION   DAOQMB010000044 DAOQMB010000000
VERSION     DAOQMB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2700_length_9805_cov_15.063385"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2445
                     /locus_tag="PLY05_02990"
     CDS             <1..2445
                     /locus_tag="PLY05_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC02298.1"
                     /translation="KALTHADTGQVLAQALRQKYPVALVDEFQDTDNYQYQILQHMYL
                     QAEARKQNCALFLVGDPKQAIYRFRGADVYTYLKAYQQVAKEQRHHLNENQRSVAPLV
                     QGLNRFFQQQADAFKQEKIVYHAVEVGQRAHSPLIDTRESPQANYQPLRFIYWPIDKV
                     TVKEQARDWAINSTVAEIIYLLTGAANGQVLLGERAVEAKDMVILVRSHFEGSLCQAE
                     LAKSGIKAALQSNKTVFESEEAEDMSRVLAAFAEPHNEGKVRAVLASRLCGATLADLV
                     TWQANAKLWTQHLIRFQNYHKLWQQKGFIVAWREFLQQEGAIARLAQLPQGERILTNF
                     LHLADLLHEEYRQRAGMRQLSDWFLQQRLEPKNSVNGELRLESDENLIRIITIHQSKG
                     LEYPIVFVPTLWNPPDPTRNTPFYHQDDEAIFNLNPELDEQAQQALRNETLSEELRLG
                     YVALTRAAQRCYIHWCPYESKDSVKNSPLGHWLVEGLTSLQKLQQQAPQLIALQAAEP
                     VELPMLKINNQANTPMTVPSAPKVAWGYRLTSFSSLQQGAKALQTDYEVLEIKDDEGK
                     EIVLEYEDQEVQLARFNFAGKKVLATSVGNCLHKIFEHADFTKPVADWQENIERQLKA
                     HGIIASWQDDVVHWLNEVMQARLMPNLRLSQLSHEYTLRELDFHLPLNQFQTTALIRC
                     LQKYGIAVGELPYQTITGFLKGSIDLVFQHQGQYFIADYKSNHLGHQLDDYQGDKLHE
                     TMFSHGYNLQAAIYSLALHRWLSSQLRDYQPERHFGGVFYLFLRGMNVHGTEGIYHWQ
                     PNLKLLDELVDLLGSY"
     gene            2581..3750
                     /locus_tag="PLY05_02995"
     CDS             2581..3750
                     /locus_tag="PLY05_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC02299.1"
                     /translation="MRLSLSHQSVLQLSLLSISMILVACNKAPQGAAPAGMPQMPPPE
                     VAVVTVKAQNVPVLFEYSAQVAASKDSEIRARVSGILLKRHFQEGQAVQAGQALFSLD
                     AAPYEAALATLEAQLSSAQARLAQAERNLTRVKPLFEQKVVAQKDYDDALSAVQIAQA
                     EVKTAQAQIKQARLNVSYTQITAPVSGLVGRTQKNEGTYISGSDILLTTISQVNPIYV
                     YFGLPDIQQQKINSLTAAGLYQAPQHNQYQAKLKLADGSVYANTGQVTFSDVGVNPST
                     GTTETRAEFANPQQHLRAGQFARIVLEGGQRLNALTVPQRAVIEGPMGKMVMIVNKEN
                     KIEPRPVKVGEWATVGKTQAWVIDSGLQTGERVLVDGLFKAMPGTVVKPVEAAGE"
     gene            3754..6930
                     /locus_tag="PLY05_03000"
     CDS             3754..6930
                     /locus_tag="PLY05_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HNC02300.1"
                     /translation="MLARFFINRPVFAMVLSIFIMLAGLAAMRNLPIAQYPEIAPPVI
                     QVTAVYPGASAEVVEQTVAAPLENQINGVDNMLYMSSTSTSQGVTTISITFEIGTNPD
                     QAAQNVNNLVKQAEARLPQEVRRQGVTVTKGSSSFLQVVAFYSPDGRYDDMYTSNYVT
                     LNVLDALKRVKGTTNVQIFGAKDYAMRIWLKPDRLAQLKLTTQEIVAAINEQNGQFAA
                     GKIGQQPTNQGQELVYTITTKGRLSEASEFENILLRTNSDGSALRLKDVARVELGAKD
                     YDFVGRINGKPATLVGIFLQPSANALEVGDNVKKTLAQLATRAPQGLSYSIPYDTTRF
                     VEVSIREVLKTLGEAMVLVFLVVFVFLQNWRATLIPTLAVPVSLIGTFAGLYLLGYSI
                     NTLTLFGMVLAIGIVVDDAIVVLENVERIMHEEHLSAYDAAVKAMHEVTSPIVAIVLV
                     LCAVFVPIAFLGGLTGELYRQFAVTIAIAVVISGIVALTLTPSLCVMILKREHKTVGG
                     VFQAFNRVFERITHHYVGGVTWMIRRGAIAALLFIGMVAIAVGLWKITPSSLVPDEDQ
                     GYYISAVILPDGATLQRTDKVVQEVLQIMKSNPANQDVVAFTGFDFLGGGFRNNAATI
                     FVTQKHWDERQVSTQQLVGELFMKTAHIKEALVLAFNPPPIMGLGQAGGFEFYIQNRG
                     EGGAAKLAEVMGQFMAAANQNKNLGGVQTLWRANSPQLRVDVDREKAHAMGVDLNNVY
                     GTLAATLGAYYVNDFNKYGRTWQVLMSAEPSYRAKPDDIGQVWVRSDKGEMIPLSALV
                     TVNYSAGPDTLDRFNNLPAVKLFGQGAMGVSSGQAIAEVEAVANKVLPSDFSYDWGGA
                     SYQEKQVSNASTIALVLGAVMVFLILAALYGQWSLPIAVLLALPFGIFGALAAVWGRG
                     FTNDVYFQIGLVTLLGLAAKNAILIVEYAIIKKQEGLSTAASAVEAARLRFRPILMTS
                     LAFILGVLPLAISHGAGAGARQSVGTGVMGGMMAATFLAIFFVPTFFKWLVDRRLSEK
                     RTAKEIHDEAAKHQHELEQKPKVMDGEA"
     gene            7136..7951
                     /gene="pssA"
                     /locus_tag="PLY05_03005"
     CDS             7136..7951
                     /gene="pssA"
                     /locus_tag="PLY05_03005"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004674635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HNC02301.1"
                     /translation="MQHKPTDEQDDDYNGITFEVVEEEANSEGKKVRNKGIYLLPNLF
                     TTAALFSGFYAIIASMQGRFEPAAIAIFCAALFDGMDGRVARMTNTQSAFGEQYDSLS
                     DLVSFGVAPAILVFSWSLHTLGKIGWICAFIYTVSAAFRLARFNVQIGSVDKRYFIGL
                     ASPLAAAIIASMVWAGTDNQIPRDMREIAIAASIITVFTGFLMVSNFKFYSFKELDRS
                     RVPFAMMLPVVLVMGIVTYDLPIGLLAVSVVYASSAPLTALFNKLRPQKSTAS"
     gene            7978..8976
                     /gene="msrP"
                     /locus_tag="PLY05_03010"
     CDS             7978..8976
                     /gene="msrP"
                     /locus_tag="PLY05_03010"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HNC02302.1"
                     /translation="MLIKHSSESPLSSEITPETIYHQRRDLLKSLGYIGVGSLLLPQA
                     YAAPVYQSAAERANAPVWLKQKIATRKLQANSTGEELTLYDSVSTYNNFYEFGLDKSD
                     PAEKADSLKTEPWTVTVEGLCNKTGTFTLEDILKPHAFEDRVYRFRCVEAWSMVVPWL
                     GFSLADLVKRFEPKTEAKYIEFITLVDKNQFPNQRTRALDWPYVEGLRLDEAMNPLAL
                     MAVGVYGRSLPAQNGAPLRLIVPWKYGFKSIKSIVKIRFTATQPKTTWAMMAPREYGF
                     YANVNPAVDHPRWSQARERRLPASLFNPNWRPTLAFNGYGKEVAAMYRNMDLRQYF"
     gene            8977..9579
                     /locus_tag="PLY05_03015"
     CDS             8977..9579
                     /locus_tag="PLY05_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HNC02303.1"
                     /translation="MVFSPKQRLISKILLFIALLTPCLWLIFAAMNNQLGADPAKKLV
                     DQTGLWAIQCLWLSLAMTPLKNWTGSSQWIQYRRMIGLFAFFYVLLHFLGYLFLLFGA
                     QWAFIGQELTKRPYIIVGFTAFILLVPLAVTSTKQWQRRLKRRWLTLHKLVYIIAVLA
                     LIHFIWLKKLGIFAVWPYALVLFILFAERIRSHLRKKQPF"
BASE COUNT         2794 a         1918 c         2165 g         2928 t
ORIGIN      
        1 aaagccttaa cccatgccga tacaggtcag gtgcttgccc aagctttgcg gcaaaaatac
       61 cctgtcgcgt tggttgatga atttcaggac accgataact atcaatatca aattttgcaa
      121 catatgtatt tgcaagccga agcgcgtaaa caaaattgtg ccttgttttt agtgggcgac
      181 cccaaacaag cgatttatcg ttttcgcggt gcagacgttt acacctattt aaaagcttat
      241 caacaagtag ccaaagagca gcgtcatcat cttaatgaaa atcaacgctc agtcgcgccg
      301 ttggtacaag gtttaaatcg cttttttcag caacaagccg atgcctttaa acaagaaaaa
      361 attgtttatc atgcggtaga agtagggcaa cgagcgcata gccccttaat agacacgcgt
      421 gaaagtcctc aagccaatta tcagccgtta cgctttattt attggccaat cgacaaagtc
      481 accgtcaaag agcaagcccg cgattgggca attaatagta cggtggcgga aatcatttat
      541 ttattaacag gcgcagccaa tggacaagta ttgttgggtg aacgtgcggt tgaagccaaa
      601 gatatggtga ttttggttcg tagccatttt gaaggcagtt tgtgtcaggc tgaattagca
      661 aaatcgggta ttaaagccgc gcttcagtcc aataaaaccg tgtttgaaag tgaagaagcc
      721 gaagatatgt cacgggtgtt ggctgctttt gccgagccac acaacgaagg caaagtacgt
      781 gccgtgttag cttcacggtt atgtggggca acgttggccg atttagtaac atggcaagcc
      841 aatgccaaat tatggacaca acatttaatt cgttttcaga actatcataa attatggcaa
      901 caaaaaggct ttattgttgc gtggcgagaa tttttgcagc aagaaggtgc aattgcacgt
      961 ttggcgcaac tgccgcaagg agaacgtatt ttaaccaact ttttgcattt agctgatttg
     1021 ttgcatgaag agtatcgtca acgggcaggc atgcgccaat taagtgattg gtttttacag
     1081 caacggctag agccaaaaaa tagcgttaat ggtgagttac gtttagaaag tgatgaaaac
     1141 ttaattcgga ttatcaccat tcaccaatct aaaggcttgg aatatccgat tgtgtttgtg
     1201 ccaaccttgt ggaacccacc tgatcccaca cgcaataccc cgttttatca tcaagatgat
     1261 gaagctattt ttaatcttaa tcctgaatta gacgaacaag cccaacaagc tctgcgtaat
     1321 gaaacgctaa gtgaagaatt acgcttgggt tatgtcgcac taactcgcgc ggcacaacgt
     1381 tgttatattc attggtgtcc ttatgaaagc aaggactcag tgaaaaactc gcctttaggt
     1441 cattggctgg tggaagggct aactagccta caaaagttac aacaacaagc accacaatta
     1501 atcgccttac aagcggcaga gcctgttgaa ttgccaatgc ttaaaattaa caatcaagct
     1561 aatacaccaa tgaccgtgcc gagtgcgccg aaagtggcat ggggctatcg tttaaccagt
     1621 tttagtagtt tgcaacaagg cgcaaaagcc ttacaaaccg attatgaagt tttagaaatc
     1681 aaagatgatg aaggcaaaga aattgttcta gagtatgaag atcaagaagt gcagttagca
     1741 cgcttcaact ttgctggcaa aaaagtctta gcaaccagtg ttggtaattg tttgcataaa
     1801 atttttgaac atgccgactt taccaagcct gttgctgatt ggcaggaaaa tatcgaacga
     1861 caactaaaag cgcatggcat tattgcctca tggcaagatg atgtggtgca ttggttaaat
     1921 gaggtgatgc aagcacgctt aatgcccaat ttgcgcttaa gtcaacttag ccacgaatat
     1981 accctacgcg agctagactt tcatttacca ttaaatcaat ttcaaaccac agcgttaata
     2041 cgttgtttac aaaaatatgg tattgcagtg ggtgagttgc cctatcaaac cattaccggc
     2101 tttttaaaag gctctatcga cctcgtgttt cagcatcaag ggcaatattt tattgccgat
     2161 tataaaagta atcatttagg ccatcagctt gatgactatc aaggcgataa attacacgaa
     2221 accatgttta gtcatggtta caacctacaa gccgccattt attcattagc cttacatcgt
     2281 tggttaagta gtcaattacg tgactatcaa cctgaacgtc attttggtgg ggtattttat
     2341 ttgtttttac gtggcatgaa tgtgcatggt acagaaggta tttaccattg gcagcctaat
     2401 ttaaagttat tggatgaatt agtagatttg cttggaagtt attaaaaaga tttattattg
     2461 caaatcttgc aacactatat tcacttttct tgtatagctc tttgtcataa aaccaagtac
     2521 attgctcggt tattgttttt tctttgcatg gctgctattg aaattttaag aggagttgtt
     2581 atgcgtttat ctttatcaca tcaaagcgtt cttcagttaa gtctattaag tattagcatg
     2641 atacttgtgg cctgtaacaa agctcctcaa ggtgcagcac ctgcgggtat gcctcaaatg
     2701 ccaccacccg aagtcgcggt cgtgaccgta aaagctcaaa atgtacctgt tttatttgaa
     2761 tatagtgcgc aagtggcggc gtcaaaagac agtgaaattc gggcgcgtgt atcgggtatt
     2821 ttattaaaac ggcactttca agaaggtcag gccgttcaag ctgggcaagc cttgttttct
     2881 ttagatgctg ctccttacga ggccgcttta gcgactttag aagcgcaatt aagcagcgca
     2941 caagcacgtt tggcacaagc tgagcgtaat ttaacccgtg ttaagccttt gtttgaacaa
     3001 aaagtagttg cccaaaaaga ctatgatgat gcgttatctg ccgtacaaat tgcgcaagca
     3061 gaggtaaaaa cagctcaagc ccaaattaaa caagcgcgtt taaatgttag ctatacccaa
     3121 attactgccc ccgtttcagg tttagtggga cgtacccaaa aaaatgaagg cacatacatc
     3181 agtggctcag atattttgtt aaccacgatc agccaagtaa atcccattta tgtctatttt
     3241 ggcttacctg atattcaaca gcaaaaaatt aatagcttaa cggccgcagg tttatatcaa
     3301 gcaccacaac ataatcaata tcaagccaag ctcaaattag ccgatggcag tgtttatgcc
     3361 aatacaggcc aagtgacttt tagtgatgtc ggggttaatc ccagcacagg cacaaccgaa
     3421 acacgagctg agtttgctaa tccacaacaa catctgcgtg caggccaatt tgcccgaatt
     3481 gtgttagagg gtggccaacg tttaaatgcc ttgaccgttc ctcaacgtgc agtcattgaa
     3541 gggccgatgg gcaagatggt gatgattgtt aataaagaaa acaaaattga accacgtcct
     3601 gtcaaggtag gggagtgggc gacagtgggc aaaactcaag cttgggtgat tgatagtggt
     3661 ttacaaacgg gtgagcgagt cttggttgat ggtttattta aagccatgcc aggtactgtg
     3721 gtaaaacctg ttgaagccgc aggagaataa accgtgttag cacgtttttt tattaatcga
     3781 cccgtctttg cgatggtgtt atcgatcttt attatgttgg ctggtttggc tgccatgcgc
     3841 aatctgccaa tcgcccaata tcccgaaatc gctccacctg ttattcaagt cacggctgtt
     3901 tacccaggtg catccgcaga agtagtagaa caaaccgttg cagcgccctt agaaaatcaa
     3961 attaatggcg tagataatat gttgtacatg agttcaacat ctacatcaca aggggtgacg
     4021 acaattagta tcacctttga aattggtact aatcctgatc aagctgccca aaacgttaat
     4081 aatttagtaa aacaagcgga agcacgttta ccccaagagg tacgtcgcca aggggtaacg
     4141 gtcaccaaag gttcgtcaag ctttttgcaa gtggtagcct tttattcgcc tgatggccgt
     4201 tatgatgaca tgtacaccag taactatgtc acgctaaacg ttttagatgc gcttaaacga
     4261 gtgaaaggca caaccaatgt gcaaattttt ggtgctaaag attacgccat gcgtatttgg
     4321 ctcaaacctg atcgtttagc ccaattaaaa ctgaccactc aagaaattgt cgccgcgatt
     4381 aatgagcaaa atggccagtt tgctgctggt aaaattggtc aacagccgac taatcaaggc
     4441 caagaattgg tttataccat taccaccaaa ggtcgtttat ccgaagcgag cgaatttgaa
     4501 aatattttat tgcgtaccaa tagtgacggc tcagccctac gtttaaaaga tgtggcacgt
     4561 gtcgaattgg gggcaaaaga ttatgacttt gttggtcgaa tcaacggtaa accagccact
     4621 ttagtgggga ttttcttaca acccagtgcc aatgctttag aagtgggtga taatgttaaa
     4681 aaaaccttag cccaattagc aacacgcgct cctcaaggat taagttactc tattccctat
     4741 gacaccaccc gttttgtcga agtgtcgatt cgcgaagtat taaaaacgct tggcgaagcg
     4801 atggtattag tattcttagt ggtttttgtc ttcttacaaa attggcgtgc tactttaatt
     4861 ccaaccttag ctgtgcctgt gtcattaatt ggcacatttg caggtttgta tttattgggc
     4921 tattcgatta acaccttaac cttatttggt atggtgttgg cgattggtat tgtcgttgat
     4981 gatgcgattg tggtgctcga aaacgttgag cggattatgc acgaagagca tttatcggct
     5041 tatgatgcgg ctgtcaaagc catgcacgaa gtcaccagcc caattgtggc gattgtttta
     5101 gtgctgtgtg cggtatttgt gccaattgcc tttttagggg gcttaacagg tgagttgtat
     5161 cgtcaatttg cggtgacaat tgccattgct gtcgtcattt caggtattgt cgctttaacc
     5221 ttaacgcctt cattatgtgt catgatttta aagcgtgaac acaaaaccgt cggtggcgtg
     5281 tttcaagcct ttaaccgtgt ttttgaacgc attactcatc attatgttgg tggtgtcaca
     5341 tggatgattc gtcgtggtgc gattgctgct ttattattta taggtatggt ggcgatagct
     5401 gtaggcttgt ggaaaatcac gcccagtagt ttagtgcctg atgaagatca aggttattac
     5461 atcagtgcgg tgattttgcc tgatggtgca accttacagc gtaccgataa agttgtccaa
     5521 gaagtcctac aaattatgaa aagtaatcca gccaatcaag atgtggttgc ttttacaggt
     5581 tttgactttt tgggtggcgg tttccgtaat aatgcagcaa cgatttttgt tacccaaaaa
     5641 cattgggatg aacgtcaagt cagcacccaa caattagtcg gcgagctgtt tatgaaaacc
     5701 gcacacatca aagaagcact tgttttagcc tttaatccac cacccatcat gggtttaggc
     5761 caagcaggtg gttttgagtt ttatattcaa aatcgtggcg agggtggtgc ggctaaatta
     5821 gcagaagtca tgggacaatt tatggcggct gcgaatcaaa acaaaaactt aggcggtgta
     5881 caaacattat ggcgtgcaaa tagcccacag ttgcgtgttg atgttgatcg tgaaaaagca
     5941 catgccatgg gtgtagattt gaataatgtc tatggcactt tggccgcaac cttaggcgct
     6001 tattatgtca atgactttaa taaatatggt cgtacatggc aggtattaat gagtgccgaa
     6061 cccagttatc gggcaaaacc tgatgatatt ggccaagtgt gggtgcgttc tgacaagggc
     6121 gaaatgattc cattatctgc tttagtcacg gttaattaca gtgcagggcc agatacgcta
     6181 gaccgtttta ataatttgcc tgcggtgaaa ttatttggtc aaggggcaat gggtgttagt
     6241 tctggtcaag caattgccga agtcgaagcg gtggccaata aagtcttacc cagtgatttt
     6301 agttatgact ggggtggagc ttcctatcaa gaaaaacaag tcagtaatgc ttcaaccatt
     6361 gccctcgtat taggtgcagt gatggtattt ttgattttgg cagcgttgta tggtcaatgg
     6421 tcattaccga ttgcagtatt gttagcttta ccgtttggta tctttggtgc tttagctgct
     6481 gtttgggggc gaggctttac caatgacgtc tatttccaaa ttggtttagt cacgctgtta
     6541 ggcttagcgg ctaaaaatgc cattctgatt gttgaatatg cgattatcaa aaagcaggaa
     6601 ggtttatcaa ccgcagcgtc agccgtagaa gccgcacgtt tacgttttcg cccaatttta
     6661 atgacttctt tagcctttat tttaggggtt ttaccattag ccattagtca tggggcagga
     6721 gcgggcgcac gtcaatcggt gggtacaggg gtcatgggtg gcatgatggc ggcaactttc
     6781 ttagccattt tctttgtgcc aacgtttttt aaatggttag ttgatcgccg tttgtccgaa
     6841 aagcgtactg ccaaagaaat tcatgatgaa gcagcaaaac atcagcatga attagagcaa
     6901 aaacctaaag tcatggatgg cgaagcctaa gtaggagcaa tccttgtggt tgctcttaaa
     6961 tgagagttga tgattagggc aacctttagg gttgcccttt tttactaaaa attcattttc
     7021 tacctgtaac attgccttaa tttgcacatc taaaacaaca aagccatagc accaaaagta
     7081 aatgctgttt atcattagac tctttacatt gttagccaaa gaagagtttc aaggtatgca
     7141 acataagccg acagatgaac aggatgatga ctacaacggt atcacctttg aagtagtaga
     7201 agaagaagcc aactcagagg gcaaaaaagt acggaataaa ggcatttatt tattacccaa
     7261 tctttttaca acagccgctt tattttcggg cttttatgcc atcatcgcct caatgcaagg
     7321 acgatttgag ccagccgcaa tcgcgatttt ctgtgcagct ttatttgatg gtatggatgg
     7381 tcgtgttgca cgtatgacca atacccaaag tgcttttggt gagcaatatg actctttatc
     7441 cgacttagtc tcttttggtg ttgcccctgc tattttggta tttagttgga gcttacatac
     7501 tttaggtaaa attggttgga tttgtgcttt tatttatacg gtaagtgcgg cctttcgcct
     7561 agcgcgtttt aatgtacaaa ttggcagtgt tgataaacgt tattttattg gtttagccag
     7621 ccctttagcc gcagcgatta ttgctagtat ggtttgggca ggcacagata atcaaattcc
     7681 acgagatatg cgtgaaattg ccattgctgc ttctattatt accgtattta caggcttttt
     7741 aatggttagc aactttaagt tttatagctt taaagaacta gaccgtagcc gtgtgccatt
     7801 tgccatgatg ttacctgtcg ttttagtcat gggcattgtg acatatgact taccgattgg
     7861 tttattagcg gtatccgttg tgtatgcctc atcggcaccg ttaacggctt tatttaacaa
     7921 attacgcccc caaaaatcga ccgcatcata agcattcatg ggggcaagga gataaaaatg
     7981 ttaattaaac attcctctga atccccatta agcagtgaaa tcacccccga aaccatctac
     8041 catcaacgcc gagatttgct caaaagtcta ggttatatcg gtgtcggttc tttattacta
     8101 ccacaagctt acgctgcacc agtctatcaa tcggctgccg aacgagcgaa tgcgcctgtt
     8161 tggttaaagc aaaaaattgc cactcgcaaa ttacaagcta acagtacagg cgaagagtta
     8221 accctctacg actcagtcag cacctataat aatttttatg aatttggttt ggataaaagt
     8281 gaccctgccg aaaaagcaga tagcctaaaa accgaacctt ggacagtcac cgttgaaggt
     8341 ttatgcaata aaactggcac atttacctta gaagatattc ttaaaccaca tgcttttgaa
     8401 gatagagttt atcgttttcg ttgtgtcgaa gcatggtcaa tggttgtgcc gtggttaggt
     8461 ttttctttag ctgatttagt caaacgtttt gagcccaaaa cagaagcaaa atatatcgaa
     8521 ttcatcaccc ttgttgataa aaaccaattt cctaatcagc gtactcgtgc tttagattgg
     8581 ccttatgtag aaggattacg tttagacgag gcaatgaatc ctttagcatt aatggcggtt
     8641 ggtgtttatg gtcgtagtct tcctgcccaa aatggtgcgc ccttacgttt aattgtgccg
     8701 tggaaatatg gttttaaaag cattaaatcg atagtcaaaa ttcgctttac agccactcaa
     8761 cccaaaacca cgtgggcaat gatggcaccc cgcgaatatg gcttttatgc caatgttaat
     8821 cctgctgttg accatccacg ctggtcacaa gctcgcgaac ggcgtttacc tgccagtttg
     8881 tttaacccta attggcgacc aactttagcg tttaatggct atggtaaaga agtcgctgcc
     8941 atgtatcgta acatggattt aaggcagtat ttttaaatgg tattttctcc taaacaacga
     9001 cttatcagca aaattttgtt gtttatcgcc ttattgacgc cttgtctatg gctaatattt
     9061 gctgcgatga ataatcaatt aggagcagac cccgctaaaa aattagtcga tcaaacagga
     9121 ttatgggcaa ttcaatgttt atggctaagt ttagcgatga caccgttaaa aaattggact
     9181 ggatcaagtc aatggattca atatcggcgc atgattggtt tatttgcctt tttttatgtg
     9241 ttattacatt ttttagggta tttattttta ttatttggcg cacaatgggc gtttattggc
     9301 caagaattaa ctaaacgacc ctatatcatt gtcggtttta ccgcttttat tttattagtg
     9361 ccattggccg tcacctcaac caaacaatgg caacggcgtt taaaacgtcg ttggctaacc
     9421 ttgcataaat tagtctatat cattgctgtt ttagctttga ttcattttat ttggctaaaa
     9481 aagctcggta tttttgctgt ttggccttat gcactggttc tatttatttt atttgccgaa
     9541 agaatcagaa gccatttacg caaaaaacag cctttttagc ctaaaaaaca cccatatagc
     9601 ctacaaaacg agcggttgat gggtttttat ttaaaaagtt caacaaagag tttgacaaga
     9661 gggcttaacg ctatataatg cgcgtcctga gtcgggcatg actggttaaa aagatgttga
     9721 aaatcaataa gttaatgatt tttagatgtg tttttagcgg attttgaagt gttgaaaata
     9781 agttgaaaaa acttgttgac aatac
//
LOCUS       DAOQMB010000045         9387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2858_length_9387_cov_15.832940, whole genome shotgun sequence.
ACCESSION   DAOQMB010000045 DAOQMB010000000
VERSION     DAOQMB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2858_length_9387_cov_15.832940"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PLY05_03020"
     CDS             <1..741
                     /locus_tag="PLY05_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC02304.1"
                     /translation="AGLFGIGGGMVIVPVLIFSFKAQGLSPTIITHLAIGTSLACIIS
                     TSISAILAQHKRGAILWPVVKKMAIWLLIGSIVGAQIANQIPAHELKILIGLFALLTA
                     AQMFSGWKGKLDNIPLPKTLGLGIAGGSIGAASAIFGIGGGTFTVPYLTLHGVAMANA
                     VASASACGLPIALGGAVGFMWSGWQTVSLPKETWGFVHLPAFIAISMTSLVFAKLGVK
                     MAHHLPAATLKKLFACLLVIVGIHFIFD"
     gene            754..2175
                     /locus_tag="PLY05_03025"
     CDS             754..2175
                     /locus_tag="PLY05_03025"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC02305.1"
                     /translation="MLLVPSDKPFDYRQPPRLSLALAALLLFLFVWLMPTEQNQKVAL
                     HEQYRQNLLTIEWPLYPTHLLQHQQAKTLDKLNEAHQNNNIAALTEQMGFDRKFVQQI
                     NEMGENYLEPEQLSTWKTARTEFDKQRDQLSSQVLGLDPQRIRPITYVTYSFLDNQSI
                     NVLISVLLLLIVGIATEWAMGSGALMGAWVLGSITTGLVYQLSHLHSVTPLIGSMGAI
                     NAILGMAFMNFRHQQSLYITGTQIALGGWLFLALFILITGINFINQQFEVSILISQMA
                     AFISGFGVYGAYKRWFMQKKNEAIQQPIIEDTHTNDQSYRQELDNLLNQLTQLNFSLA
                     EENARQLLESYPNDKRLQEILYHLCKFRSNALEFEELACTLFNQTSSATNNTTALRIY
                     NDYKKRSKSFVALDANTCSQLAIRFANIHAFKEAEEVFKRGFDHKHQSPLLKKAALML
                     EKMFSSQQQEQKAKYYQNIAAKL"
     gene            complement(2165..3583)
                     /locus_tag="PLY05_03030"
     CDS             complement(2165..3583)
                     /locus_tag="PLY05_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HNC02306.1"
                     /translation="MTTQYCKYHPLSQANWYCSTCQMSLCDDCIQPSVEREVLPTCLL
                     CSKEVSSLQQAEAVVPFWLQYTHFMRLPLSLLGIFLLIVLFVFPQLFPDNLMLLAALM
                     AYSVSALYGWSLMQQSATGELKDINLQTLVKQTKGLALQISLVMGVVLTGLDWLSLHY
                     SKVAMLLNIAAAIVFPAMLISATVDKSLSALFKVDQIKQVISSLRFFYVPVFMVSVVL
                     WLISFAVISLLADVLMPLTAQGLNQSLHAYGVWVIMSVVGYVLFQFQQVLGIESQAKH
                     KKRSVIAKQTDKQIARLEVYLKEGAFDKAASLLKTLAEKQPNNSDIQEKYYQLLVFMK
                     DTELAPLQASNYMDALLQNGQKQQALQVFLKLKRLIPDFKPASPEMSFDLAKAFMETG
                     GYQQVVDLLTDLHKEHPHFVALPEAYLLLSRVLYEKMNNQPLALETVEYLVNRFQKHP
                     RFELIDKVWRSLGGKPKQDFII"
     gene            complement(3932..4531)
                     /locus_tag="PLY05_03035"
     CDS             complement(3932..4531)
                     /locus_tag="PLY05_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02307.1"
                     /translation="MQNVFADPNHPHQPYLLSIKLAPAFCVLNPERQKTKQCQEGYAI
                     IVHGLWKEKNVVKEESPCTEQPPELSPVQQQVLERIMPDEEMRNKAWQRYGACTGMSA
                     QEYFRVIMNYANKLRLPNIFKEESRDDRSFDQEYLVQEIQKININLPEKGFYLRCQTK
                     QDKTFLTEVRVCYDKLGQFAECKTYQPNCPQLVTLRAVK"
     gene            complement(5450..>6562)
                     /locus_tag="PLY05_03040"
     CDS             complement(5450..>6562)
                     /locus_tag="PLY05_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC02308.1"
                     /translation="PIMLSVQGSDLKELQRISQEIADGMRKIDGLVDIESSLKASKPT
                     VAVELNRDAASDAGLSVASIGNALRPLLAGDAVTTWQDAQGENYDVRVRLPESERQQV
                     TDLSNLHFASTRKDPQTDAPYMIPLGQVATFEQSEGAAQINRRNLMREVLIQANTDGR
                     PAGDIGVDVKKLQDSIKLPAGYRFETEGANKDMAESAGYAATALLLAIIFIYMVLGSQ
                     FNSFLYPVAIMTSLPLSLIGVFMALFVFSSTLNLFSIIGIVMLMGLVTKNAILLIDFI
                     KVAIEKGDDRTHAIMMAGRTRLRPILMTTMAMVMGMMPLALGLGDGAEQRAPMAHAII
                     GGVITSTLLTLIVVPVVYTYLDDIKKLVKRALTKKV"
     assembly_gap    6563..6662
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6663..8251)
                     /locus_tag="PLY05_03045"
     CDS             complement(<6663..8251)
                     /locus_tag="PLY05_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC02309.1"
                     /translation="MWLTRVSVQNPVFAAMMMFALMVLGLFAYKDVGVEEFPNVEFPF
                     VIVYTNYPGASPEVVESDVTRKVEDSVNTISGVKRVISTSYEGRSFVAIEFYLNVPVT
                     VAVQDVRDKIAVLKTTFRDEIKDPIVERYNPGSVPVLSLAFTSTTLPMRELSTYIDQK
                     ISKRLQTVSGVGKVEVLGAVKREVRINLKPQALQAYRVGVDEVLRVLQLENSELPSGT
                     IKSGNQEQVVQIKGRLAEPEQFKRLIVARRQGMPIYLEQVADVVDGEQDLESAALING
                     QPAVSLDVIKSSGANVIEVVDKANETLAEIRQQLPAGVSMTVVADSSKSIRASLADVQ
                     KTLLEGAALAILIVLLFLGSWRSTVITGLTLPISLLGTIFAIYYFGFTINLMSLMALS
                     LCIGLLVDDAIVVRENIVRHAAMGKSHYQAALDGTNEIGLAVLATTLTIIAVFLPVAF
                     MGGIIGRFFFQFGVTVSAAVLLSMFVSFTLDPMLSSVWPDKHKHGERRTGFGGLLDKF
                     SDFLEWLGDIYSKLIAWSLRHR"
     gene            complement(8257..>9387)
                     /locus_tag="PLY05_03050"
     CDS             complement(8257..>9387)
                     /locus_tag="PLY05_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC02310.1"
                     /translation="IDIYLEGKRTMFKPKLIYVAVVATLLVTACGEEKTSATATVLPA
                     TPASLELAKTDVATVVEQRLQKSVSLTGQLQALNYTTVQSEVNASVAQVLVREGETVK
                     KGQVLVQLATQDLQARAKQAEAALASAKAESVLANAVKERNEQLYKDHYISDIDYKRG
                     IAEAQARSENVKAQESLLSIAKKALNDTVIASPISGVVAKRHVQAGQMVAMNSPVLNV
                     VDLSQLELVASIAPEHLASLQVGQTLQFNVQGFSENFSAKVSRINPVADAATRAVTFY
                     GTVQNPQAQLKAGLFVQGVLALGSATQGLVVPKVALRHEQNQDFIWIIETNKLTKRAV
                     KVGIIDDKTGLALISQGVQLNEKVVLAQLNAQAVNMPIKIVE"
BASE COUNT         2865 a         1950 c         1660 g         2812 t
ORIGIN      
        1 gctggcttat ttggtattgg tggaggcatg gtgattgtgc ctgtactcat ttttagtttt
       61 aaagcacaag gtcttagtcc aacgattatt actcaccttg ccattggtac atccttggca
      121 tgtattatta gcacctctat cagtgctatt ttggcacaac ataaacgtgg ggctattttg
      181 tggcctgttg tcaaaaaaat ggctatttgg ctactgatag gttctattgt tggtgcacaa
      241 attgccaatc agattcccgc acatgagtta aaaattctta ttggcttatt cgctttatta
      301 actgccgcac aaatgttttc tggttggaag ggtaaactag acaatatccc tctaccaaaa
      361 accttaggct taggtattgc tgggggaagt attggtgcag cttctgctat ttttggtatt
      421 ggtggtggca catttaccgt tccttaccta accttacatg gtgtagcgat ggctaatgcc
      481 gtagccagtg cttcggcttg tggtttgccc attgctttag gcggtgctgt aggttttatg
      541 tggtcaggat ggcaaactgt tagcttaccc aaagaaacat ggggttttgt tcatctcccc
      601 gcatttattg cgattagcat gaccagcctt gtctttgcca aactgggtgt aaaaatggcg
      661 catcatttgc ctgcagcaac attaaaaaaa ctatttgcgt gtttattagt gattgttggc
      721 attcacttta tttttgatta acaaggaaaa atcatgctgc ttgttcctag tgataaacca
      781 tttgattatc gtcaacctcc gcgtttaagc ttggcattag cggcactttt gcttttttta
      841 tttgtttggt taatgcctac cgaacaaaat caaaaagtag ctcttcatga acaatatcga
      901 caaaatctgt taactataga atggccattg taccccacac atttattaca acatcaacaa
      961 gcaaagacct tagacaaact gaatgaagca caccaaaaca acaatattgc tgcattaact
     1021 gagcaaatgg gttttgatcg aaaatttgtg caacaaatta atgagatggg cgaaaattac
     1081 ttagaacccg aacagttgag cacatggaaa actgctcgca ctgaatttga caaacaacga
     1141 gatcaactta gcagtcaggt tttaggactt gatccccaac ggattcggcc aatcacttac
     1201 gtcacctact cttttttaga caatcaatct atcaacgtgc ttatctcggt tttgttgtta
     1261 ttaattgttg gcattgcaac tgaatgggca atgggaagtg gtgcattaat gggtgcatgg
     1321 gttttgggca gtatcactac tggccttgtc tatcaactat cgcacttaca cagcgtaacg
     1381 ccattaatag gcagtatggg tgctattaat gccattttag gtatggcgtt tatgaatttt
     1441 cgtcaccaac aaagtttata tataacagga acacagattg ctttaggtgg ttggctattt
     1501 ctagctttat ttatactcat tacagggatt aactttatca atcaacaatt tgaagtatct
     1561 attttaatca gccagatggc agcctttatc agtggatttg gtgtttatgg cgcatataaa
     1621 cgttggttta tgcaaaagaa aaatgaagcg atacaacaac ccattatcga agatacacat
     1681 actaatgatc aaagctatcg ccaagagtta gacaacttac ttaaccaact gacacaatta
     1741 aattttagcc ttgcagaaga aaatgcacgg cagcttttag aaagttatcc aaatgataaa
     1801 cgcttgcaag aaattcttta tcacttatgc aaattccgct caaatgctct agaatttgaa
     1861 gagctcgcct gtacactatt caaccaaact agtagcgcga ctaataacac taccgcatta
     1921 cgtatttata acgattacaa aaaacgtagt aaaagctttg tggccttaga tgctaatacc
     1981 tgctctcaat tagccattcg ttttgccaat attcacgctt ttaaggaggc agaagaagtc
     2041 tttaaacgtg gctttgatca taaacatcaa tcacccttgc ttaaaaaggc cgcattaatg
     2101 ttagaaaaga tgttctcatc gcaacaacaa gaacagaaag caaaatacta tcaaaatatt
     2161 gcagctaaat tataaagtct tgtttaggtt tacctcctaa gctacgccaa actttatcta
     2221 ttagctcaaa acgtggatgt ttttgaaaac gattaactaa atactcaacc gtttccaaag
     2281 ctaatggttg attattcatt ttttcatata acactcttga taataataaa tatgcttctg
     2341 gtaaggcgac aaaatgtgga tgctctttat gtaaatccgt taataaatca accacttgct
     2401 gataaccccc cgtttccata aatgcttttg ccaaatcaaa gctcatttct ggactagctg
     2461 gcttaaaatc aggaataagg cgtttcaatt taagaaaaac ttgtaaagct tgttgttttt
     2521 ggccattctg caacaaagca tccatataat ttgatgcttg caaaggtgct aactcggtat
     2581 ctttcataaa tacgagtaat tgataatatt tttcttgaat atcactatta ttaggctgtt
     2641 tctctgctaa agtttttaat aaacttgcgg ccttatcaaa tgccccttct tttaaatata
     2701 cttccaatcg tgctatttgc ttatcggttt gcttagcgat taccgaacgt tttttgtgtt
     2761 tagcctgcga ctctatgcct aatacttgtt gaaattgaaa caacacataa cccaccacag
     2821 acattatgac ccaaacgcca tacgcgtgta atgattgatt gagtccctga gcagttaatg
     2881 gcattaacac atcagccaat aaactaatca ctgcaaaact aattaaccaa agtaccactg
     2941 ataccataaa aacaggtaca taaaaaaatc ttaatgaaga aatcacctgt ttaatttgat
     3001 ctactttgaa taatgcacta agacttttat ccacagtcgc actaatcaac attgcaggaa
     3061 aaacaatagc agccgctata tttaagagca tagctacttt actataatgc aacgacaacc
     3121 aatctaatcc tgtcagcaca acccccatca ctaaggatat ttgtagagct aatcctttgg
     3181 tttgtttgac aagtgtttgt aagtttatat cttttaactc tcctgttgca gactgctgca
     3241 ttaatgacca gccatataat gcagacacgc tataagccat caatgctgca agcaacatta
     3301 agttatctgg aaaaagctgc ggaaagacaa ataaaacaat taataaaaaa atccccaata
     3361 aactcaaagg caaacgcata aaatgtgtat attgcaacca aaaaggtact actgcctctg
     3421 cctgttgtaa actactcact tctttactgc acaataagca agttggtaat acttctcgct
     3481 caacggatgg ttgaatacaa tcgtcacaca agctcatttg acaagtagaa caataccaat
     3541 ttgcttgaga tagcggatga tatttgcagt attgggtagt catataaatt cacttgtatt
     3601 tttacataga caaaatagtg ctaagtgtag gagagcttga tctatggcgc aatcaaaaaa
     3661 atcagggtaa ttacgtcata acagaggaca attaatctaa cgaattgatc acaattttat
     3721 gcaagatatg gatactaaca tcaataaatc gttacagact gtgactctaa gcagatagaa
     3781 tttaatgaag atttgattag gcgtgtaaaa aagtaacaac atacttagaa gctgttgata
     3841 ttttgcgtat gcaccgcaat caaaacgtca atagcctctg tatttcactc caaatttttt
     3901 aaacagtttt gatcactgct ttctgaatac cctacttaac agctctgagc gtcactagtt
     3961 gaggacaatt aggctgataa gtcttacact ccgcaaattg tcctaacttg tcataacata
     4021 ctctgacctc tgtcaaaaaa gttttatctt gtttagtttg acaacgtaaa taaaaacctt
     4081 tttcaggtaa attgatattt attttttgaa tttcttgaac taaatattct tgatcaaatg
     4141 aacgatcatc tctgctttct tctttaaaaa tattaggtag gcgaagtttg tttgcataat
     4201 tcataattac ccgaaaatac tcttgtgcag acatacctgt acatgcccca taacgctgcc
     4261 atgccttatt acgcatttcc tcgtctggca taattctttc taaaacttgt tgctgcacag
     4321 gtgatagctc tggaggctgc tcagtacaag gagattcttc cttaacgaca tttttttctt
     4381 tccacaaacc atggacaata atggcatatc cttcttggca ttgttttgtt ttttgtcttt
     4441 caggatttaa tacacaaaat gctggtgcta atttgataga taataagtat ggctgatgag
     4501 gatgattagg gtctgcaaat acattttgca tgcagaatac ccctgataaa caagccaaaa
     4561 gagcagaaat tggctggtat tttctcattc acaaacctct tagtttcgca aaactcgaat
     4621 caaacacaaa actttttgga cttacgcggc tatcacttat ttgttcacac ttcaaccgtt
     4681 atcaacgatt tcatattcac aaaaacgtac ttgtttcgac ttcgttcaac acgagctttc
     4741 agagaaatac gtcagtcctg atttttgaat atgctaaacg atcatcttaa aacatgttgt
     4801 ttatcgttgt aattttaata gcatttcact tggtgctata ttgtctgtat aaaggtattg
     4861 caaaacatct ttttttgctg ttggtttttg ttattaggct gttattttta cgttatttta
     4921 gaagacttat tttgtcgtaa aaatactaca tttaacgtag tattttatcg aaaaatagtc
     4981 atttttcttc acaacaaaat attaacctgc tgttttaaaa acaaaaaata aaaaacatag
     5041 atagagttgt ggttatttag ttggctggga gtacttgtag ttaatcttct ttatgttaat
     5101 ataaacacaa caaggttgtt gcaggatttt tggacaagac attcatgatg aatgcaacaa
     5161 ggccgttaaa gattagctag gacgctgata gtacttggaa gtacaaaagg aagtggcaag
     5221 aagctactaa ggattcgtct ggaagcgtca gtggaaatag gaaatggact acctgccgag
     5281 aggcaagttt aaatggaaag gaaacacaag gttgtgtaag gaacagcggg atggaaacaa
     5341 atcggggcag tttatctgcc ccgcttgttt ttgggctaca ctaaactcaa atatctatgc
     5401 gtaaattctt aacaagggat agactgtaat tatctatccc ctttatcaac taaactttct
     5461 tcgtcaaagc cctctttacc aattttttaa tatcatccaa ataggtataa acgacaggca
     5521 caacaatcaa ggttaatagt gtggacgtaa ttaccccacc aataatcgca tgagccatgg
     5581 gtgcgcgttg ttctgcacca tcacccaagc ccaaagcgag cggcatcatt cccatcacca
     5641 ttgccatggt ggtcattaaa atcgggcgta aacgggtacg acctgccatc atgatggcat
     5701 gagtacgatc atcacccttt tcaatcgcca ctttaataaa atcaattaat aaaatcgcat
     5761 ttttggtgac taagcccatg agcatgacaa taccaataat cgagaataaa ttgagcgtac
     5821 tgctaaacac aaataaggcc atgaatacgc caattaacga taagggcaat gaagtcataa
     5881 tcgcaacagg ataaagaaaa ctattaaatt gtgagcctaa gaccatgtaa ataaagataa
     5941 tggctaacaa taatgcagta gctgcatatc cagctgactc cgccatatct ttatttgcac
     6001 cttcggtttc aaaacgatac cctgcgggta acttaatact atcctgtagt tttttcacat
     6061 cgacaccaat atcacccgca ggacgaccat cggtattagc ttgaattaac acttcacgca
     6121 ttaagttgcg gcggttaatt tgtgctgccc cttcgctttg ttcaaaagtc gccacttgtc
     6181 ctaaaggaat catatagggg gcatcggttt gtgggtcttt gcgcgtacta gcaaaatgta
     6241 gattacttaa atcagtcact tgttgacgtt cactttcggg caaacgcact cgcacatcat
     6301 agttttcacc ttgagcatct tgccatgtgg ttaccgcatc acctgccaat aaaggacgta
     6361 aagcattacc aatagaggca acacttaaac ccgcatcact cgctgcatca cgattgagtt
     6421 ctaccgccac cgtcggttta ctggctttta atgacgattc aatatccacc aaaccatcaa
     6481 ttttgcgcat accatcagca atttcttgcg agatacgttg taactcttta aggtcagaac
     6541 cttgcacact gagcataatc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nncgatgacg taatgaccat gcaattaatt tgctataaat atcgcccaac cactccaaaa
     6721 agtcagaaaa tttatctaac aagccaccaa agcctgtacg tctttcacca tgtttatgtt
     6781 tgtctggcca aacagaggac aacatcgggt caagggtaaa gctgacaaac atggacaaca
     6841 ataccgccgc actgactgtc accccaaatt gaaagaagaa acggccaata ataccgccca
     6901 taaaggcaac aggtaaaaac accgcaataa tggttaaggt cgtggctaag accgctaaac
     6961 caatttcatt ggtgccatcc aaagcggctt gataatggct tttgcccatt gctgcatgac
     7021 gcacaatgtt ttcacgcacc acaatcgcat catcaaccaa caaaccgata cacaatgata
     7081 aagccattaa cgacattaaa ttgatggtaa aaccaaaata ataaatggca aaaattgttc
     7141 ctaataacga gatgggcaaa gttaagccag taatcaccgt gctacgccat gaccccaaaa
     7201 ataacaagac aattaaaatc gctaacgctg ccccttctaa taaggttttt tgcacatcgg
     7261 ccaaagaggc acgaatagac tttgagctat ccgccaccac cgtcatgctt acacctgctg
     7321 gtaattgttg gcgtatttcg gcaagggttt cgttggcctt atcaaccact tcgatgacgt
     7381 ttgcacctga acttttaatc acatctaaag acacggcagg ttgaccattg attaatgccg
     7441 cagactctaa gtcttgctca ccatcaacta catccgccac ttgctctaaa taaattggca
     7501 ttccttgacg acgcgccaca attaaacgct taaattgttc tggctcagcc aaacgccctt
     7561 tgatttggac aacttgctct tgattacctg atttaatcgt gcctgaaggc aattcagaat
     7621 tttcgagttg taatactctt aaaacttcat ctacacccac acgatacgct tgtagggctt
     7681 gtggttttaa gttaatgcgg acttcgcgtt taaccgctcc caacacttca actttaccca
     7741 cacccgacac ggtttgtagg cgtttactga ttttttggtc aatataggtg cttaactcac
     7801 gcataggcaa agttgttgag gtaaatgcca acgacaatac tggcactgaa cctggattat
     7861 aacgttcaac aatcgggtct ttaatttcat ctctaaaggt ggttttgagt accgcgattt
     7921 tatcgcgcac atcttgcacc gccaccgtga caggcacatt gagataaaac tcaatagcca
     7981 caaaagaacg tccttcataa gacgtagaaa ttactcgttt gacacctgaa atagtattaa
     8041 cactatcttc aactttgcgc gtaacatccg attccaccac ttcgggcgat gcaccggggt
     8101 aattggtata aacaatcaca aagggaaatt cgacattggg aaactcttcc accccaacat
     8161 ctttataggc aaataaaccc aataccatta atgcaaacat catcatggca gcaaatacag
     8221 ggttttgcac actgacacgt gttaaccaca taattattac tccacgattt taatgggcat
     8281 attaacggct tgtgcattaa gctgggctaa caccactttt tcattcagtt gcacaccttg
     8341 gctaattaag gctaatcccg ttttatcatc aataatgcca accttaactg ctcgtttagt
     8401 taatttattt gtttcaataa tccaaataaa atcttgattt tgttcatgtc ttaaagcgac
     8461 tttaggcaca actaaacctt gtgtggctga gcctaaagcc aaaacacctt gcacaaataa
     8521 acctgctttt aactgggctt gtggattttg taccgtgcca taaaatgtga ctgcgcgtgt
     8581 tgccgcatcg gcgacagggt taattcgact cactttggca gaaaagttct cgctaaatcc
     8641 ttgcacatta aattgtaaag tttgaccaac ttgtaacgag gctaaatgtt cgggtgcaat
     8701 actagctacc aattctaatt ggctcaaatc cacgacattc agaacagggc tattcattgc
     8761 aaccatttgc cctgcttgta catgacgctt ggccaccaca ccactgattg gtgacgcaat
     8821 caccgtatca tttaaggctt ttttagcaat actcaataaa gactcttgcg ccttgacgtt
     8881 ttcactgcgt gcttgggctt cggcaatgcc acgtttgtaa tctatgtccg aaatataatg
     8941 gtctttatat aattgctcgt tacgttcttt aacagcattg gctaacacag actctgcttt
     9001 tgctgaggct aatgctgctt cggcttgttt agcacgggct tgtaaatctt gggtagccaa
     9061 ttgcaccaat acctgacctt tttttactgt ctcaccttct cgcactaaga cttgagcgac
     9121 actcgcattt acttccgatt gaaccgtggt gtaattgagg gcttgtaatt gacctgttaa
     9181 agacacactc ttttgcaaac gttgctcaac aacagttgcc acatcggttt tagcaagttc
     9241 taaactggca ggtgttgctg gtaatacggt tgctgttgca ctggtttttt cttcaccgca
     9301 agccgtcact aataatgttg ccacaacagc aacatagatt aatttgggtt taaacatggt
     9361 tcttttaccc tctaaataaa tatcaat
//
LOCUS       DAOQMB010000046         9196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2933_length_9196_cov_17.449951, whole genome shotgun sequence.
ACCESSION   DAOQMB010000046 DAOQMB010000000
VERSION     DAOQMB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2933_length_9196_cov_17.449951"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..1119
                     /locus_tag="PLY05_03055"
     CDS             157..1119
                     /locus_tag="PLY05_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HNC02311.1"
                     /translation="MYDSPTLLDLVTAKIRLKHYSIRTEEAYKNWIKRFILFHQKQHP
                     RQLNAFHIEQFLSYLATDKNVSASTQNQALSSIVFLYKDVLCMDMPELENISRAKKPK
                     RLPVVLTPDEIKQLLAQLDGTIWLIASLLYGSGMRLLECLRLRVKDIDLKTHQIIVRD
                     GKGQKDRITMLPKSLVKPLAVHLEKVRLLHCQALENGYGTVYLPFALDKKYPNANSEW
                     AWQYVFPAKTASTDPRSDTKQRHHLSEQVIQRAIKNAISAANIYKPASCHSLRHSFAT
                     HLLESGYDIRTIQELLGHNDISTTMIYTHVLNKGGKGVISPLDR"
     gene            complement(1100..2248)
                     /locus_tag="PLY05_03060"
     CDS             complement(1100..2248)
                     /locus_tag="PLY05_03060"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNC02312.1"
                     /translation="MQQLILTFNVGSSSLKFSCYQQEKPLFSLNIDLKQQQFLGQGQF
                     QIKNWTPSYHLADDACFIVDHIKTHYAPLNLIIAHRVVHGAEHQQAVMITPDILQELE
                     ALSPLCPLHQPPALTVINAIQNKWPDIQQIAAFDTSFHQSQSRIARSFALPKRLTSKG
                     IKRYGFHGLSCQSIIGQLANTQRDKGRVIVAHLGNGSSITAIKDGVSQANSMSFSTLD
                     GLPMGTRCGHLDAGVMLYLVEQGWTAQSLTELLYYQSGLLGLSGISSDMRVLMDSQET
                     EAKFAIDYFVDYCARTIASMATTIQGIDRLVFTGGIGEHQSTIRANIVEHLTWLGLKI
                     NPQANEQHSFIISTDDSTAELLCLPTNEQEQLFLAAKQLVKLSNDQED"
     gene            2414..>3877
                     /locus_tag="PLY05_03065"
     CDS             2414..>3877
                     /locus_tag="PLY05_03065"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC02313.1"
                     /translation="MNSEWLYLSASQLLIQLKNGSISSRALLETYISRINVKNPAVNA
                     VVAMNLEKARARADEADAARARGESWGVLHGLPITIKDTFEVPDMPCTAGAPEFRHYF
                     PKQAAAAVQRLIDEGAIVFAKTNVPLFASDVQSYNKIYGTTNNPWNLRYTPGGSSGGA
                     AAALAAGFTALELGSDIGGSIRTPAHFCGVYGHKPTHGIISLRGHIPGPPDTQKTPDL
                     AVAGPMARTAEDLALMLDVVVGANPDDAIAWQIKLPKAKQKTLQDFRVLLWIDDSLCP
                     IDNEMRQIYLSLFEKLKAAGVNVQIGAPLGMSLNTFYPLYLRQLGSVMALSQSTLQRN
                     LMGVAAPVLAKVGKYVKAPPLFENFLQGMAQSYANWHYGHEHSLRLRKKFSQVFNEYD
                     VILMPPTPSTTIPHQQKPEMPQRRITVNGEQRSYTELFTWIAPATLLGLPATSAPLGI
                     TRSGLPVNIQIMGARFHDKTTIKFASLLSKLMGGFTPP"
     assembly_gap    3878..3977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3978..4479)
                     /locus_tag="PLY05_03070"
     CDS             complement(<3978..4479)
                     /locus_tag="PLY05_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02314.1"
                     /translation="MKGQTVFSKWLTLMLLSVTDCSVTYAQALPTVSYSATQIIETVP
                     GPFSNKIYMAANKERQDANLGGAIVSTIIRHDKGVAWLLIPSSKHYQEIDIKTVGLAS
                     VHRLLEQGNKIELGYQHLNNTKTKKFAVYTEQETAPSFLWLSEQGILLKAEIPKNLAT
                     GRPQATI"
     gene            complement(4495..5700)
                     /locus_tag="PLY05_03075"
     CDS             complement(4495..5700)
                     /locus_tag="PLY05_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNC02315.1"
                     /translation="MKKTLMSILLTTTAMLSQMNTALADSQAVVFVGNNWDGTVNVFN
                     SQNYSKVGVINVVPDKQARMKEILLNPIKLVAFQAVRNLIGEGHDQLVDDMYSSNDGK
                     LLIASRPSFADVVAIDIATGKIVWRFVVDGYRSDHMALSPDGTKVAVSASTGNVVHIL
                     DVNTGKQVGKFASGNSPHENVYSKDGSKIYHASIGYVFTPLDPYFFDWTKGERVFQVV
                     DANNYQIIKKFNMADKLKAAGLAHLSPAVRPMAHTPDEKFFYFQLSFLHGFVEYDMVN
                     DKVTRLAKLPNLVPNMPLEQYVNDSAHHGIAMNSAGTTLCVAGTMDDYVAIVDRATFN
                     YKILKGLGEKPYWVTSNKTGDRCYVSWSGTDQMSVISYNTGTEVARINVGDHPQRIRE
                     GYAPAGWMN"
     gene            complement(5837..6928)
                     /locus_tag="PLY05_03080"
     CDS             complement(5837..6928)
                     /locus_tag="PLY05_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02316.1"
                     /translation="MKKQQITPKKSDKNSQSFRKIAMFSSVGLIFASLGLLIWVDSNN
                     SENFAKSMQQSDKSTQQTNNYAPVTAQVPLANISMDSLNAEEKQLGTNLQKRYGSKLN
                     HPYWRLQTIESLKRYLMEKYPNDWMARLKAMLKIFFPAEYDKLLASLDAMESYNDWMA
                     QLKYSTTFSSQEEKLRAIWDKRIQLFGEDAKIIWQAQLKQEKVDATLQQLDKSNLPLN
                     TKVDRYVQTLVDVYGKEAVDPEKSHPVQQMEGLLTLSSVQDELHSLAPEQRKQELHHL
                     RKVMGLDDAAIKRWDELDAERAQRAEAGASYMQERAALVKQYEGETLKVHIQALQLRK
                     FGAEEAQYIRNEEEGGFYRFQERQQIGIN"
     gene            complement(6925..>7638)
                     /locus_tag="PLY05_03085"
     CDS             complement(6925..>7638)
                     /locus_tag="PLY05_03085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC02317.1"
                     /translation="PFEISMNDSPRIKTHLALVPAGGEVRYVPEDGILACCFLDVLSR
                     DYQHLAKRMKPVLDGLYVLDNDLFAAQIINKLRDLYFNPIEPTLVYEQFVQALDLPLP
                     QPEGQVQESIAKVVNSVRLSATENLTNGHYAAEAGISEEVLARWFRQVTGLTLRRYRN
                     WHRLFLAAWLMQQGVSLTEAAHEVGFSDAAHFNHVFREMLGLKPSFIQRAMAVTRIYL
                     GGMLAIDGTVMYQEEGNNP"
     assembly_gap    7639..7738
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7955..9163
                     /locus_tag="PLY05_03090"
     CDS             7955..9163
                     /locus_tag="PLY05_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02318.1"
                     /translation="MNYKKLATLAASVLLSLGLSLPAQQAHAEAQKTKYPIILAHGMA
                     GWDEIVGLDYFGNEKGVFALDGCQLLEVNGCNDDVNGSQMAEAFQVTSLADSETRGLQ
                     LADKVQSYMTTKGVSYVNLVGHSQGGFDIRKAAHLLKSRKGYTVVNNLISISSPHRGS
                     PYAKKLIDKYALNGSSFCWNLAWDGTPANDPCGVLISKIADVLFDAVNQQSVTTRNNI
                     IAAGKQLIYADYDPKDGVTTGAKAFNAKYNLKNSAGQYVATRFRSLITAQDDGNRNPV
                     ITALHTLLGFNADGDGYCVDDCDNDGAAGKGNGSIYDTDDDGLVGINSQQMGGRLQYN
                     SILGVLDTLTETSITAVDDINNPSSIAMTSKTGIITQDHLDVVGVGPDTFDELEFYAA
                     INDYIAKAGG"
BASE COUNT         2750 a         1742 c         1655 g         2849 t
ORIGIN      
        1 cgtcactttt gacgcataaa cagatatacg gcaaaaatga cgtataatcc actaattttt
       61 tgcagcctaa agctaggacg tgcctatgtt gaaaaatact gcacgaaaag ttaggataag
      121 taaagtggtt tatttgaaga tcaaggaaga aagcaaatgt atgactcacc cacattatta
      181 gatttagtaa cagccaaaat tcgtctcaaa cattatagta ttcgaactga agaagcttat
      241 aaaaattgga ttaaacgatt tattcttttt catcaaaaac aacatcctag gcaactaaac
      301 gcctttcaca tagaacagtt tttaagctat ttagcgactg acaagaatgt ttcggcatca
      361 actcaaaatc aagcattatc atctattgtt tttctataca aagacgtttt atgtatggat
      421 atgcctgaac ttgaaaacat cagccgagct aaaaaaccta aacgcttacc ggtggttcta
      481 actccagatg aaattaagca gttattagct caattggatg gaacaatttg gttaattgca
      541 tcattgttat atggcagtgg tatgcgctta ttagaatgtc tccgtttaag ggtaaaagac
      601 atagatctaa aaacacatca aattatcgtt agagatggta aaggccaaaa agatcgtatt
      661 accatgctgc caaaatcact tgttaaacct ttagctgtac atttagaaaa agttcgcctt
      721 ctacactgtc aagcattaga aaacggttat ggtaccgtat atttaccttt tgcattagac
      781 aaaaaatatc ctaatgctaa ctctgaatgg gcatggcaat atgtttttcc agctaaaaca
      841 gcctcaacag acccaagatc agatactaaa cagcgtcatc atcttagtga acaagtcatt
      901 caacgggcga ttaaaaatgc catatctgct gcaaatattt ataaaccagc aagctgtcat
      961 tctttaaggc actcttttgc tacgcattta ttagaaagtg gctatgatat tcgcactatt
     1021 caagagttat taggtcataa tgatataagt acaacaatga tttataccca tgttttaaac
     1081 aaaggtggga aaggagtcat cagtcctctt gatcgttaga caatttgacg agttgcttag
     1141 cggctaaaaa aagttgctct tgctcattag ttggcaagca taatagctct gccgttgagt
     1201 catcagtcga aattataaaa ctatgttgct catttgcttg agggttaatt tttaagccca
     1261 gccaagttaa atgctcaact atatttgctc gaatggtgga ctgatgttca cctatcccac
     1321 ctgtaaaaac taatctatct attccctgta tcgttgtagc cattgaagca atggttcttg
     1381 cacaatagtc aacaaaataa tcgatagcaa atttagcttc tgtctcctga ctatccatca
     1441 aaacacgcat atctgagcta atacctgata aacctaataa tcctgattgg taatacaata
     1501 attctgttaa ggattgtgca gtccatccct gctcgactaa atataacatc acacccgcat
     1561 ctaaatgacc acaacgtgtt cccatcggta gtccatctaa tgtactaaaa ctcatactat
     1621 tcgcttggct aactccatct ttaatagcag taatacttga gccattcccc aaatgagcga
     1681 caataacacg acctttatct cgctgtgtat ttgctaactg gccaataata gattggcaag
     1741 ataatccatg aaatccataa cgtttaatgc ctttacttgt aagtcttttt ggcagagcaa
     1801 aactacgtgc tattcttgat tgtgattgat gaaaagaagt atcaaaagcg gcgatttgtt
     1861 gaatatcagg ccatttattt tgtatagcat tgatgactgt caaagcaggt ggctgatgta
     1921 aaggacataa tggcgagagt gcctcaagct cttgcaagat gtctggcgta atcatcactg
     1981 cttgttgatg ctctgcccca tgcacaactc gatgggcaat tatgaggttt aaaggtgcat
     2041 aatgtgtctt gatatggtca acgataaaac aagcatcgtc agccaagtga taacttggtg
     2101 tccaattttt aatctgaaat tgtccctgtc ctaaaaattg ctgctgcttt aaatctatat
     2161 ttaatgaaaa taatggtttt tcttgttgat aacaggaaaa ttttagtgag gatgatccaa
     2221 cgttaaatgt cagtattaat tgttgcatta gcacaccatg ttttattagt gagtgtatga
     2281 gtaattatca agttgatttt taatagggta tattggccta aaataaccat ctttattacc
     2341 ctagtgcata gttactgctt ttgacagaat agtctaatat ctcactaata cttcaatgac
     2401 ataggataat atcgtgaatt ccgaatggtt atatctttct gcaagtcaat tattaattca
     2461 actaaaaaat ggctcaatca gtagtcgtgc cttattagaa acctatattt cacgcattaa
     2521 tgttaagaat cctgcggtga atgctgtcgt tgcaatgaat ttagaaaaag ctagagcaag
     2581 agccgatgaa gctgatgcag ctagggctag aggcgagtct tggggtgttt tgcatggctt
     2641 accaatcacc attaaagata cctttgaagt acccgatatg ccatgtacag ctggtgcgcc
     2701 cgagtttcgt cattattttc ccaaacaagc cgctgcagca gtacaacgtc taattgatga
     2761 gggagcaatt gtttttgcta aaaccaatgt tcctttattt gcttccgatg tgcaaagtta
     2821 taacaaaatt tatggtacaa ccaataatcc ttggaattta cgttatacac caggcggatc
     2881 ttctggtggt gcagccgctg cattagcagc aggatttacg gctcttgaac tggggagtga
     2941 tattggtggt tcaattcgta cacctgctca tttttgtggc gtgtatggtc acaaacctac
     3001 acatggcatt atttccttac gtggccatat tccaggccca cctgataccc aaaaaacacc
     3061 agatttagcg gtagctgggc caatggctcg cacagctgaa gatttggcct tgatgttgga
     3121 tgtggtcgtt ggtgccaatc ctgatgatgc gattgcatgg caaataaaac tgcccaaagc
     3181 caaacaaaaa actttacaag attttagggt gttactgtgg attgatgata gtttatgccc
     3241 gatagacaat gaaatgcgac aaatctattt gagtttattt gaaaaattaa aagcagcagg
     3301 tgttaatgtt caaattggcg caccattagg catgagttta aatacgtttt atcctttata
     3361 tttgcgtcaa ttgggcagtg ttatggcgtt atcacaaagt acattacaac gtaatttaat
     3421 gggagtcgca gctcccgtat tagctaaagt tggaaaatat gttaaagctc cgcctttatt
     3481 cgaaaacttt ttgcaaggga tggctcaaag ttatgctaac tggcattatg gccatgagca
     3541 tagtttaaga ttacgcaaaa aatttagtca ggtatttaat gagtatgatg tgattttaat
     3601 gccaccaaca ccttcgacaa ccattcccca tcaacaaaaa ccagaaatgc cacaacgtcg
     3661 tattacagtt aatggtgaac aacgcagcta taccgaatta tttacttgga tagcacctgc
     3721 aacattactg ggtttacctg caacaagcgc gccgttaggc attacgcgta gtggtttacc
     3781 tgttaatatt caaattatgg gagcacgttt tcacgataaa acaaccatca aatttgctag
     3841 tctattgagc aagttgatgg gtggttttac gccacctnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnata tcgtggcttg tggtcgacca gtagccaagt ttttgggaat
     4021 ctctgctttt aataaaatcc cctgttccga caaccataaa aaacttggtg cggtttcctg
     4081 ttcagtataa acagcaaatt tttttgtttt ggtattatta aggtgctgat agcctaattc
     4141 aattttattg ccttgttcca ataaacgatg gacagacgct aatcctaccg tttttatatc
     4201 aatttcttga taatgcttgc tacttggaat aagtaaccaa gccacacctt tgtcatgacg
     4261 gataatcgtt gaaacaatag ctcctcctaa atttgcgtct tgacgctctt tgtttgcagc
     4321 catataaatt ttattgctaa acgggccggg tacagtttca ataatttgag tagctgagta
     4381 tgagaccgtt ggtaatgctt gtgcatacgt cactgaacaa tcagtgacgc ttaaaagcat
     4441 cagtgtcagc cacttagaaa aaacagtttg tcctttcatg gttttcaccg ttttttaatt
     4501 catccaaccc gcaggtgcat aaccttcacg aatacgttgc ggatggtcac caacattaat
     4561 ccttgctact tctgtgcctg tattatatga aatcaccgac atttgatcgg tgccactcca
     4621 tgagacataa caacgatcac ctgttttatt agatgtgacc caataaggtt tttcgcccaa
     4681 accttttaaa attttatagt taaatgtggc acgatcaaca attgcaacat aatcatccat
     4741 tgtgccagca acacataatg ttgttcctgc actgttcatg gctataccat gatgtgcaga
     4801 atcattaaca tattgctcta acggcatatt gggaactaag ttaggtagtt ttgccaaacg
     4861 agttactttg tcattgacca tatcgtattc tacaaaacca tgtaaaaatg ataattggaa
     4921 ataaaagaac ttttcatctg gcgtgtgagc cattggcctt actgctgggc ttaaatgcgc
     4981 taaacccgcg gcttttaact tatccgccat attaaatttt ttgatgattt gataattatt
     5041 ggcatcaacg acttgaaaca ctctttcgcc tttagtccaa tcaaaaaagt atgggtctaa
     5101 tggggtaaaa acatagccaa tactggcatg ataaattttt gagccatctt tggaatagac
     5161 attttcatgc ggggaattgc cagaggcaaa tttgccgact tgtttgcctg tgttaacatc
     5221 taaaatatgc acgacattac ccgttgaagc agaaaccgca acttttgttc catctggaga
     5281 taatgccatg tggtcagaac gataaccatc aacaacaaaa cgccacacga tttttcctgt
     5341 ggcaatatca attgccacca catctgcaaa actaggacga gaagcaatta ataatttacc
     5401 atcattgctg ctatacatat catcaacaag ttggtcatgt ccttcgccaa ttaaattacg
     5461 tacggcttga aatgctacta atttaattgg gttaagcaaa atctctttca tgcgtgcttg
     5521 tttatctggt acaacattaa ttacgcctac tttagaataa ttttgactat taaaaacatt
     5581 gaccgttcca tcccaattgt taccaacaaa aacaaccgct tggctatcgg ctaaagcagt
     5641 attcatttgc gaaagcatgg ctgtagtcgt caataaaatc gacattaatg tttttttcat
     5701 tattatttgc ccctgagatt ttttagatta ataaaccaaa gacagtttct gcctaaggca
     5761 atttcatggg cgtgatagta gttaaagctt tatttagttt cccccctctt tagtagggtt
     5821 tcgacaaagg gtagttttaa tttatgccaa tttgctgacg ttcttgaaaa cgataaaatc
     5881 cgccctcctc ctcattacga atatattgtg cttcttctgc gccaaactta cgtaattgca
     5941 aggcttgaat atgtactttt agggtctctc cttcgtattg tttgactaat gctgcacgct
     6001 cttgcatata agatgcacct gcttcggcac gttgagcacg ttctgcgtcc aattcatccc
     6061 aacgtttaat tgcggcatca tctaagccca ttactttacg caaatggtgt aactcttgtt
     6121 tgcgttgctc aggtgctagg ctatgtagtt catcttgtac tgaacttaat gttaataagc
     6181 cttccatttg ttgaacagga tgtgattttt cgggatcaac ggcctcttta ccataaacat
     6241 caactaatgt ttgcacataa cgatcaactt tggtgttaag gggcaaatta gatttatcta
     6301 gttgttgtaa agtggcatct actttttctt gttttaattg tgcttgccaa ataattttgg
     6361 catcttcgcc aaataactga atacgcttat cccaaattgc acgtagcttt tcttcttgag
     6421 aactaaaggt tgttgaatac tttagctgtg ccatccaatc gttataactt tccatggcat
     6481 ctaaagaagc caataactta tcgtactccg caggaaaaaa tatttttaac atcgctttta
     6541 aacgagccat ccaatcatta gggtattttt ccattaaata acgttttaaa gattcaatcg
     6601 tttgtaatcg ccaataagga tgattcaatt tgctgccata acgtttttgt aaatttgtcc
     6661 ctaattgttt ttcttctgca tttaagctat ccattgatat attggctaat ggaacttgtg
     6721 ctgtgactgg agcataatta tttgtttgtt gagtgctttt atctgactgt tgcatagatt
     6781 tagcaaaatt ttcactattg ttactatcaa cccaaattaa caatcctaga ctagcaaata
     6841 ttaaaccaac gctactaaac atcgcaattt tacgaaaact ttgactattt ttatctgatt
     6901 ttttgggggt tatttgttgt tttttcatgg attattaccc tcttcttgat acatgaccgt
     6961 accatctatt gctaacatac cacctaaata aattcgggtt acagccattg ctcgttgtat
     7021 aaatgaaggt ttaagaccta acatttctct aaaaacatga ttaaagtgtg ctgcatcaga
     7081 aaaaccaact tcatgagccg cttctgttaa cgaaacacct tgctgcatca accatgccgc
     7141 taaaaataaa cgatgccaat ttctatatct acgcaaggtt aaaccagtta cctgtctaaa
     7201 ccaacgggct aatacctctt cagaaatacc tgcttcagca gcataatgac cattagttaa
     7261 attttctgtt gctgataatc gtactgaatt tacaactttt gcgattgatt cttgaacttg
     7321 accttctggc tggggcaaag gtaaatctaa ggcttggaca aactgttcat aaaccaatgt
     7381 aggctctata gggttaaaat ataaatctcg taatttatta atgatttgtg ctgcaaataa
     7441 atcgttatct aaaacataca aaccatctaa tacaggcttc atacgttttg ctaaatgctg
     7501 ataatcacgg ctcagtacat ccaaaaaaca acaggccaaa ataccatcct ctggcacata
     7561 acgcacttcc ccccctgctg gcacaagggc aagatgggtt ttaatacgcg gactatcatt
     7621 cattgaaatt tcaaatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnct
     7741 aaatattatg taaatcacgt caatttttct tgtaagtttt tcgcttttta tcaatacatt
     7801 tttatttttc cgacaaacgg cagaaaatct tacaagtaat attttgcgtt gaagcttatc
     7861 ttttatacac acaaggggct aaaaaacaac ctaatacata gtaaagccct catcaacaat
     7921 gaaaactaat aaacaacaac aaaggtaagc cactatgaat tataaaaaac tggccacact
     7981 ggctgcttca gtgcttttat cactgggttt gtcacttcca gctcaacagg cacatgccga
     8041 agcacaaaaa acaaaatacc cgattatttt agctcatggt atggcaggtt gggacgaaat
     8101 agtaggtctt gattattttg gtaatgaaaa gggtgtattt gcactagatg gatgccaatt
     8161 actagaggtt aatggctgta acgatgatgt taatggcagt caaatggcag aagcatttca
     8221 agtgacatca ttagctgatt cagaaacacg gggtttacaa cttgctgata aagttcaaag
     8281 ctacatgaca actaaagggg tgagttatgt caatttggtc ggacattctc aaggtggctt
     8341 tgatattcgc aaagcggctc acttgttaaa atcccgtaaa ggttatactg tagttaataa
     8401 cctgattagt atttcttcac ctcatcgtgg ctcaccttat gccaaaaagc tgattgataa
     8461 atatgcttta aatggctcat cgttttgttg gaatttagcg tgggatggta cgcctgctaa
     8521 tgacccttgt ggggtgttaa ttagtaaaat tgctgatgtt ctttttgatg cagtgaatca
     8581 acaaagtgtg accactcgta acaacatcat tgctgcgggt aaacaactca tttatgctga
     8641 ttacgaccct aaagatggtg tgacaacagg tgctaaagcg ttcaatgcaa aatataactt
     8701 aaaaaacagt gcggggcagt atgttgccac tcgtttccgt tcactaatta ctgcacaaga
     8761 tgatggtaat cgtaatcctg tgattacagc attgcacaca ttattaggtt ttaatgcaga
     8821 tggcgatggt tattgtgtag atgactgtga taatgatggg gcagcaggca aaggcaacgg
     8881 ctccatttat gacactgatg atgacggttt agttggtatt aactctcagc aaatgggtgg
     8941 tcgtttacaa tataactcta ttttaggtgt gcttgatacc cttactgaaa cctctattac
     9001 tgcggtagat gacattaata atccatcatc catagccatg acctctaaaa ctggtattat
     9061 cactcaagat cacttagatg tggttggtgt tggccctgac acctttgatg aattagagtt
     9121 ttatgcggct attaatgatt atattgccaa agcaggtggt tgattttatc taattgaatg
     9181 gtcacgggga tcacaa
//
LOCUS       DAOQMB010000047         9116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_2970_length_9116_cov_15.711842, whole genome shotgun sequence.
ACCESSION   DAOQMB010000047 DAOQMB010000000
VERSION     DAOQMB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9116
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2970_length_9116_cov_15.711842"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(349..1554)
                     /gene="tyrS"
                     /locus_tag="PLY05_03095"
     CDS             complement(349..1554)
                     /gene="tyrS"
                     /locus_tag="PLY05_03095"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNC02319.1"
                     /translation="MMTPEQQLELIKRGTADIIQETELLAKLKEGRPLRVKAGFDPTA
                     PDLHLGHTVLINKLRVFQDLGHEVIFLIGDFTGMIGDPTGKNVTRKPLTQEQVKENAK
                     SYQEQVFKILDPQKTRIVFNSEWMGKKTAADLIQLAAQYTVTRMLERDDFSKRYANQQ
                     PIAIHEFLYPLLQGYDSVELQADVELGGTDQTFNLLMGRTLQARYGQSSQICLTLPIL
                     EGLDGVQKMSKSLGNYIGVNDSAGEMYQKILSMPDSLIPRYFELLSFRPMAEVNQFLQ
                     EIADGRNPQDIKKVLAEEIITRFHNAEAAINAHKGAGNTLAYGEIPEDVPEVIINLEA
                     GVTDLPISAILNRAKLVKNAASAKDVLARGVVFVDGQAVNADYRMTLGQSKVIQAGKK
                     AIAKVTLQD"
     assembly_gap    1716..1815
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1816..2607
                     /locus_tag="PLY05_03100"
     CDS             <1816..2607
                     /locus_tag="PLY05_03100"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HNC02320.1"
                     /translation="QTPFTLQIGDPNVIAEKTKMTVVADFRRRDVAAGGQGAPLVPAF
                     HKALLKSSSIDRVVLNLGGMANITVLPSQKDILVYGYDTGPANVLIDAWCQQHLQQNY
                     DEDGRWAASGTVIPELLEKLLAHSFFAKAAPKSTGREDFHLAWLEDVLSNFEHYDLAD
                     VQATLLELTTTSVANEINKIGFAQGELYLCGGGAYNPILWQSLAHKLPNYQLFSTEKI
                     GISPTWIEATAFAWLAQQTIKNLTGNLPAVTGASGKRILGGIFQA"
     gene            complement(2643..2984)
                     /gene="erpA"
                     /locus_tag="PLY05_03105"
     CDS             complement(2643..2984)
                     /gene="erpA"
                     /locus_tag="PLY05_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HNC02321.1"
                     /translation="MSELAQGMILTDRAAAKVRKLREGESNPNLKLRVYITGGGCSGF
                     SYGFTFDENSNESDSIFENEDVTMLVDSLSFQYLVGSEVDYVEGLEGSRFVIKNPNAT
                     TTCGCGSSFSI"
     gene            complement(3162..>3815)
                     /locus_tag="PLY05_03110"
     CDS             complement(3162..>3815)
                     /locus_tag="PLY05_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005045351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC02322.1"
                     /translation="LSSPKIAPSMTSGSYEVVGIMPLGAGYLLVNDRAINNLSKVSGK
                     KIAVLDYDKSQAKMVQKIGAQPVASDVTNFAGKFNNGQVDIVAAPAVAIKPLELYKGL
                     GTKGAIVRFPIVQITGNIIINPKKFPEGFGQKSREYVATQVDKAFKTIEKTEKDVPAK
                     YWMDVAEADKPGYVKLMRESRIALAADGIYDKKMLSLLKRVRCKHDPASFECALKDE"
     assembly_gap    3816..3915
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4294..4635)
                     /locus_tag="PLY05_03115"
     CDS             complement(4294..4635)
                     /locus_tag="PLY05_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02323.1"
                     /translation="MGELKDVAESQVEVKLVDRVKDLGNQVSLASLGVVSKVEEVVAK
                     VEEEGQKQLDKLVAAGQAARGEQAANDKKVVLVAVGLVETLKVEADKLKVEAEKLFND
                     LVAAGEKRKAA"
     gene            complement(4941..6047)
                     /locus_tag="PLY05_03120"
     CDS             complement(4941..6047)
                     /locus_tag="PLY05_03120"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004729017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HNC02324.1"
                     /translation="MKIGIFGGGQLGRMMALAAYPFNLGFSFYESAANCPSAPLGEVI
                     GDKEASQASLDAFLASADVFTYEFENIPVAWVEHIAQHKPVYPSVKSLAVSQNRLNEK
                     QLFAKLGIPAAAYHQVCSQADLQQAVDTLGLPLVVKTTMGGYDGKGQFVIRQAEQVAQ
                     AWSELQAGAPLIAEAFIQFNRELSIIAVRAQDGETRCYPLAWNTHHQGILSHSVVPAP
                     QVDAATQAQAEQYITAILHELNHVGVLTLEMFQTEQGLYANETAPRVHNSGHWSIEGA
                     ICSQFENHMRAVAGLPLGSTQELRPSAMINIIGQHPKTSDILAIPEAHLHLYGKSERV
                     GRKLGHITVTADCLDTLNIRIQQIAAVLPNKMAL"
     gene            complement(6044..6559)
                     /gene="purE"
                     /locus_tag="PLY05_03125"
     CDS             complement(6044..6559)
                     /gene="purE"
                     /locus_tag="PLY05_03125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009506226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNC02325.1"
                     /translation="MVMTATTATPLVGIIMGSQSDWATMQHAADVLRTLGVPFEAEVV
                     SAHRTPDKLFAYAEAARGRGIQVIIAGAGGAAHLPGMCASKTSLPVLGVPVQSQALNG
                     LDSLLSIVQMPAGVPVATLAIGKAGATNAGLLATQILAAQYPQYLQAIENFRQQQTDK
                     VLANPIPGEQA"
     gene            complement(6668..7009)
                     /locus_tag="PLY05_03130"
     CDS             complement(6668..7009)
                     /locus_tag="PLY05_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004672205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HNC02326.1"
                     /translation="MPITLQTILLLICSNIFMTFAWYGHLKNLQDKPWYLAAIFSWCI
                     ALFEYLLMVPANRIGHSVLSVGQLKIIQEVITLSVFVPFAILYLKEPFKMDYVYAGLC
                     LLGAVYFMFRN"
     gene            7169..7726
                     /locus_tag="PLY05_03135"
     CDS             7169..7726
                     /locus_tag="PLY05_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02327.1"
                     /translation="MKALLQLFWQILRFKRGPEDVPYAPNLLWLLIVVNLTISISAQS
                     LGRPNQFNIAITLPIISLCVEALVIFSLLQLKQLANRFTQTLITILGIDSFLTLLTIP
                     LLIIGFNMIGHTPLLKFLGLLEVILLGWSIAIRAFIYHRALNIGLLLGNILAVMVLLL
                     TLSINVKVFPELLEQAKAAANQSSR"
     gene            7740..9107
                     /gene="mpl"
                     /locus_tag="PLY05_03140"
     CDS             7740..9107
                     /gene="mpl"
                     /locus_tag="PLY05_03140"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HNC02328.1"
                     /translation="MHIHILGICGTFMGSLAVLAKNLGHKVTGSDANVYPPMSTQLEA
                     QGISLMQGYEPEHLQPRPDLVIVGNAMKRGIPAVEYVLNEGIPYTSGPQWLAQYVLQG
                     KHVLGVAGTHGKTTTTTMVAWILEQAGLEPGFLIGGVPKGFEASARLGGGKYFVVEAD
                     EYDSAFFDKRSKFVHYAPKTAILNNLEFDHADIFDDLAAIQKQFHHLVRTIPSTGKII
                     VPANDDNLKDTLAKGCWTPVETTGIAPNSSDWQAELRANDGSHFVVSYQGQQAEVNWS
                     MTGLHSVYNGLAAVAAAHNVGVDLVTACAALSAFDGVKRRMELVGEVADISVYDDFAH
                     HPTAIKTTLEGLRNKVGDRKIWAIIEPRSNTMRMGTHKDNLAESARDADQVIWYQPAG
                     LDWDLSPVIAASPVPAQVANNIDGIIADVIAQVKSGDAIVIMSNGGFGGIHGKLVNAL
                     RAKFA"
BASE COUNT         2647 a         1777 c         1716 g         2776 t
ORIGIN      
        1 accaccccca acctctcaac agcctcagcc atcaagtcag gccgcgcatt ctatcaattt
       61 aaaacttaat gtcaagcatt attttctaaa tttttcagca acttaagttt tattaaactt
      121 aacatttgct cgcgtcctga agaggaggcg aattatatag aacacaaaac ctttgtcaac
      181 caagaaaaga cttgtttgcc tactacttga tcaaaaatat gttttatgac tataaaacac
      241 actaaaaagc attgatgggc ttaagattag ccttgaatat tcattttttt aggtaaataa
      301 acagccaaag tctaaaaaag cccaattttt gagaaggtat ctttcagctt agtcttgtaa
      361 tgtcactttg gcaatagctt ttttgcctgc ttgaataact ttactttggc ctaatgtcat
      421 tcgatagtcg gcatttactg cttgcccatc aacaaacaca actccccgtg ctaatacatc
      481 ttttgcggat gctgcatttt ttacgagttt tgctctgttt aagatggcac taataggtaa
      541 atctgtaacg ccagcttcta aattaatgat aacctctggt acatcctctg gaatttcccc
      601 ataagctaaa gtattacccg cacctttatg tgcatttatt gcggcttctg cattatgaaa
      661 gcgggtaata atttcttcgg ccaatacttt tttgatatct tgagggttac gaccatctgc
      721 tatttcttgg aggaattgat tgacttctgc cattggtcta aagcttaaca attcaaaata
      781 acgaggaatt aagctatcag gcattgatag aattttttgg tacatctccc ctgcactatc
      841 atttacccca atatagttac ctaatgattt agacattttt tgtacgccgt ctaaaccctc
      901 tagaataggt agagtcaaac aaatttgtga ggattgtcca taacgtgctt gtagcgtacg
      961 ccccattaat aaattaaagg tttgatctgt gccacctaat tcaacatctg cctgtaactc
     1021 aacagagtca tagccttgta ataagggata taaaaattca tgaatagcaa tgggttgctg
     1081 attagcataa cgtttggaaa aatcatctcg ttccaacata cgtgttactg tgtactgtgc
     1141 ggctaattga atcagatcag cagcagtttt tttccccatc cattcagagt taaaaacaat
     1201 acgggttttt tgtgggtcta gaattttaaa gacttgctct tgatagcttt tagcattctc
     1261 tttcacttgt tcttgcgtta aaggtttacg ggtgacattt ttgcctgtcg gatcgccaat
     1321 catgcctgta aagtcaccga ttaagaaaat gacttcatgt cccaaatctt gaaacacccg
     1381 taatttgtta attaatacgg tgtgtcctaa atgtaaatct ggtgctgttg ggtcaaaacc
     1441 tgcttttaca cgtaaggggc ggccttcttt aagtttggcc aataattcag tttcttgaat
     1501 aatgtcggct gtaccacgtt taatcaattc aagttgctgt tcaggtgtca tcatgttgtt
     1561 aactacgcta gaataaaaat ttgcgttatt ttagggcgaa tagctataaa ttgcattatg
     1621 ctctacaagt ttttaaaaaa atgagtgttt acaatggatg agatttattt aggtttgatg
     1681 tctggtacta gtctggatgc ggttgatgcc gttgcnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnncaaac acctttcacc ctacaaattg gtgatcccaa tgtgattgct
     1861 gaaaaaacca aaatgacagt tgtcgctgat tttcgccgtc gtgatgttgc tgctggtgga
     1921 caaggtgcgc ctttggttcc tgctttccat aaagccttat taaaaagctc ttctatagat
     1981 agagtcgtac ttaatttagg tggcatggct aatattacgg ttttgcctag ccaaaaagat
     2041 attttagtgt atggctacga tacaggccct gctaatgtgt taattgatgc ttggtgtcag
     2101 caacacttac aacaaaacta tgatgaagat ggtcgatggg cagcctctgg cacagtgatt
     2161 cctgagttac tcgaaaaact actagcacat tccttttttg cgaaagctgc tcccaaaagt
     2221 acggggcgcg aagattttca cttagcatgg ttagaagatg tgttaagcaa ctttgagcat
     2281 tacgatttgg cggatgttca agcgacttta ttagagttaa ccacgacttc tgttgccaat
     2341 gagattaata aaattggttt tgcccaaggt gagttgtatt tatgcggcgg tggtgcatac
     2401 aaccccattt tatggcaatc tttagcccat aaactaccta attaccaact attttctaca
     2461 gaaaaaattg gcatatcgcc aacttggatt gaagcaacgg cttttgcatg gttagcccaa
     2521 caaacgatta aaaacttaac aggtaactta cctgccgtca caggtgcaag tggtaaacgt
     2581 attttaggtg ggatttttca ggcttaataa ttaaaggagg acttagtcct cctttaataa
     2641 atttagatag aaaaagacga accacagcca cacgttgttg tggcatttgg atttttaatc
     2701 acaaaacgcg aaccttctaa accttcaaca taatcgactt ctgaacctac taaatattga
     2761 aagctcaacg aatcaactaa catcgtcaca tcttcatttt caaaaataga atcgctttcg
     2821 ttggagtttt catcaaaggt gaaaccataa gagaaaccag aacagccgcc acctgtgata
     2881 taaacacgta gtttaagatt aggattggat tcaccttcac gcaatttacg tactttagcc
     2941 gctgcacggt cggtaagaat catgccttga gccagctctg acataaaaca gcctcatcat
     3001 aaatgggcat atctgcccca aaagttaaat ttgactaaat tactaggaat tatcttgcgt
     3061 tagctatcaa ccgtcaactc gaatttgttg ctttatgagt cgtgaaaaag aaaaagccca
     3121 gcatttgctg ggctttggca tctctttaga taagtaaaag attactcgtc ttttaatgca
     3181 cactcgaagc ttgctggatc gtgtttacaa cgtacacgct tcaataaact taacattttt
     3241 ttgtcgtaaa taccgtcagc agccaaagca atacgggatt cgcgcattaa tttgacgtaa
     3301 ccaggcttat cggcttctgc aacgtccatc caatatttag ctggaacatc tttttcagtt
     3361 ttttcgatag ttttgaatgc tttgtcaact tgagttgcta catattcgcg gctcttttga
     3421 ccaaaacctt ctgggaattt cttaggattg ataatgatgt tgccagtaat ttgtacgatt
     3481 gggaaacgta caatagcacc tttagtgccc aaacctttgt acaattctaa tggtttgatt
     3541 gcaaccgctg gtgcagcaac gatgtctact tgaccattgt tgaatttacc agcaaagtta
     3601 gttacgtcag aagcaactgg ttgtgcacca attttttgta ccattttagc ttgagatttg
     3661 tcatagtcta aaacagcaat ctttttacca gaaactttag ataagttgtt gatagcacgg
     3721 tcatttacta ataaataacc agcgcctaat ggcataatac ctacaacttc ataagaacca
     3781 ctggtcatgc ttggtgcaat tttggggctg cttaannnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnatata ttttaaaaaa tcaaactttt gagcttcccc tcagtgagtg
     3961 ctgattattt ttacaggaga atgtaaaaag tcactatcaa agctcaataa aagtttgggc
     4021 gattttacct aacaaaagca atgttaaaca tagtttttgt taaagacatt ggtaatttat
     4081 atgaaaaatt actttttatt tttaaccaat ataaaccata aacctcattt gtatagatag
     4141 atacgctttt ttagaaatat tttgtaaaat tttagctaaa aaaaagggtg gcagggtgcc
     4201 accccaaaag agggttcttt tgaatagaca gcgcaacctg cctaaatatg cgtcactcaa
     4261 atcttaataa acataagatc taagtgttaa caattaagca gctttacgct tttcgccagc
     4321 agctactaaa tcattaaata acttttcagc ttcaactttt aatttgtcag cttcaacttt
     4381 taaagtttca actaaaccaa cagcaactaa cacaactttt ttgtcattag cagcttgttc
     4441 gccacgagca gcttgaccag cagcaactaa tttgtctaat tgcttttgac cttcttcttc
     4501 aactttagca accacttctt caactttaga cacaacgcct aaactcgcta aagaaacttg
     4561 attacctaaa tctttaacac gatcaaccaa ttttacttca acttggcttt ctgcaacgtc
     4621 ttttaactcg cccatgacta tctccgagga atttaataca ccaacactca ttacctaaaa
     4681 gttaatgttt gaattaagaa ctccgtttag taactctacc gttgtttatt gcaagcaata
     4741 tcacagggta caaacattaa actttttcta tcagctcgct cagtgatgat ttactgcgct
     4801 gctgtcttga tggcgattat agaggggcaa ttagcgacta aagcatgtat ttgtttgaca
     4861 atcaaacaag ataaacgttt ttttattcat ccaagataaa agctcgttaa acacgagctt
     4921 tggagacaat ctatagcaag ttataaagcc attttattgg gcaataccgc cgcaatttgc
     4981 tgaatgcgga tgttgagagt gtctaaacag tctgcagtca cagtaatgtg gcctaattta
     5041 cgaccaacac gctcactctt accatacagg tgtaaatggg cttcaggaat ggccaaaatg
     5101 tcagaagttt ttggatgttg gccaatgata ttaatcatag cacttggtcg tagctcttgc
     5161 gtgctgccta atggcaaacc tgctactgca cgcatatgat tttcaaattg cgagcaaatc
     5221 gctccctcaa tactccaatg acctgaatta tgcactcgtg gtgcagtttc gttagcatat
     5281 aaaccttgtt ctgtttggaa catttctaag gttaatacgc ccacatgatt taactcatgc
     5341 aaaatagcag tgatgtattg ctctgcttgg gcttgtgttg ccgcatcaac ttgtggtgca
     5401 ggaacaacag agtgcgacaa aattccttga tgatgggtat tccacgctaa aggataacaa
     5461 cgtgtttcac catcttgtgc acgcactgca ataatagata actcacggtt aaactgaata
     5521 aatgcttctg ctattaaagg cgcacccgct tgtaactctg accatgcttg cgccacttgc
     5581 tctgcttgac gaataacaaa ttgtcctttg ccatcataac cacccatcgt ggtttttacg
     5641 actaatggta aaccaagagt atcgactgct tgttgtaagt cggcttgtga gcatacttgg
     5701 tgataagctg ccgcaggaat gcctaatttg gcaaataatt gtttttcatt taaacgattt
     5761 tgtgaaacag ctaaagattt aacacttgga taaacaggct tgtgttgagc aatatgttct
     5821 acccatgcaa ctggaatatt ttcaaactca taggtaaata catcggcact ggccaaaaaa
     5881 gcatctaagc tggcttggct agcctctttg tcgccaatca cctcacctaa tggagcagat
     5941 ggacaatttg ctgctgactc ataaaaacta aaaccaagat taaaggggta tgctgccaaa
     6001 gccatcattc ggcctaactg accgccacca aaaatcccaa ttttcatgct tgctctcccg
     6061 gaatagggtt agctaagact ttgtcggttt gttgttggcg aaagttttca atcgcttgta
     6121 agtattgtgg atattgtgcc gctaaaattt gggtagccaa taaacctgca ttggttgccc
     6181 ctgctttgcc aatggctaag gttgcaacag gcacacctgc gggcatttgc acaatagata
     6241 gtaaagaatc taaaccatta agtgcctgtg attgcacagg aacgcctagc acaggtaagg
     6301 atgttttaga ggcacacata ccaggtaaat gcgctgcccc ccctgcccct gcaataatta
     6361 cttgaatgcc acgaccacgt gcagcttcgg cataagcaaa taatttgtcg ggagtacgat
     6421 gtgcagacac aacttctgcc tcaaaaggta caccgagggt gcgtaataca tcggcggcat
     6481 gttgcatggt tgcccaatcc gattgtgaac ccataataat accgactaag ggagttgccg
     6541 ttgttgctgt catgaccaaa gttctctaga aacaccttta cagttttgca cggaaaggcg
     6601 ggtaagttta aaaagccgac tattttggct gaataattac cctaggccaa gtgtttatgt
     6661 aaaaaattta gtttctaaac atgaaataaa cggcacctaa taaacataaa cccgcataaa
     6721 cataatccat tttgaaaggt tctttgagat ataaaatggc aaacggcaca aaaacactca
     6781 aagtaatgac ttcttgaata atttttaact gccctactga taagacacta tgaccaattc
     6841 tattagcagg caccattaat aaatactcaa aaagcgcaat acaccaacta aaaatagccg
     6901 ctaaatacca cggtttatct tgtagatttt tcagatgacc ataccaagca aaggtcataa
     6961 agatattgga acaaattaat aataggattg tttgtaaggt aatcggcata acaagtctct
     7021 aagtttaggt cttacgcagt tatcgcttag ttgctcatag ctcaatgatt ttatattcac
     7081 aaaatgcgct aatttcggtg taatgcgtca gttctgagtg ccattaattc gctataattc
     7141 ggcgataatt ttttaggata ttggctaaat gaaagcccta ttacaacttt tttggcaaat
     7201 tctacgattt aaacgcggcc ctgaagatgt gccttatgcc cccaatttgt tatggctatt
     7261 aattgtagtg aatttgacga ttagcatcag cgcacaatca ttaggcagac caaatcaatt
     7321 taatattgcc attactttac cgattattag cttatgtgtg gaagctctcg ttatttttag
     7381 cttattacaa ttaaagcaat tggctaatcg ttttacccaa acattgatca ctatcttagg
     7441 cattgatagt tttttaaccc tactgacgat ccctttatta attattggct ttaacatgat
     7501 tggccataca cctttattaa aatttttggg attattggaa gttattttat tgggatggag
     7561 cattgctatt cgagccttta tttatcatcg tgctttaaat attggcttat tgctcggtaa
     7621 tatcttagcg gtcatggtac tcttattaac cttatcaatt aacgttaaag tatttcccga
     7681 actcttagaa caagccaaag cggctgctaa tcaatcttct cgttaggaca caagatatta
     7741 tgcatattca tattttaggt atttgcggca catttatggg gtcattagcc gttttagcta
     7801 aaaatttggg ccataaagtg acaggtagtg atgcgaatgt ttatccaccc atgagcactc
     7861 aactagaagc tcaagggatt agtctgatgc aaggttatga accagaacat ttgcaacctc
     7921 gccctgattt agtcattgtt ggcaatgcca tgaaacgtgg cattcctgca gtagaatatg
     7981 tgttaaatga aggcattcct tatacttctg gcccacaatg gttggcgcaa tatgtgttac
     8041 aaggcaaaca cgttttaggc gtggctggga cacatggcaa aacaaccaca acaacgatgg
     8101 ttgcttggat tttagaacaa gcaggtttag agccaggttt tttgattggt ggtgtaccca
     8161 aaggttttga agcaagtgca cgtttaggtg gtggcaaata ttttgtcgtt gaagccgatg
     8221 aatatgattc tgcgtttttt gataaacgct ctaagtttgt tcattacgcc ccaaaaacag
     8281 cgattttaaa taatttagaa tttgaccatg ctgatatttt tgatgattta gccgctattc
     8341 aaaaacaatt tcatcattta gtccgcacta ttcccagcac aggcaaaatt attgtccctg
     8401 ccaacgatga taatttaaaa gacactttag ccaaaggctg ttggacgcct gtggaaacaa
     8461 caggtatcgc ccccaattct agcgattggc aagctgagtt gcgtgccaat gatggcagtc
     8521 attttgtggt gtcttatcaa ggccaacaag ccgaagtaaa ttggagcatg acaggtttac
     8581 acagtgttta taatggttta gccgccgttg ctgctgcaca taatgtgggc gttgatttag
     8641 tgactgcttg tgcggcatta agcgcctttg atggggtaaa acgtcgcatg gagttggtgg
     8701 gtgaagtggc cgacattagc gtttatgatg attttgccca ccacccgact gcgattaaaa
     8761 ctaccttaga aggcttacgc aacaaggttg gcgaccgcaa gatttgggca attattgagc
     8821 cgcgctcaaa caccatgaga atgggtacac acaaagataa tttagctgaa tcggctcgtg
     8881 atgctgacca agtaatttgg tatcagcccg caggcttgga ttgggattta agcccagtca
     8941 ttgcggcttc gcctgtgcca gctcaagttg ctaataatat tgatggcatt attgctgatg
     9001 ttattgctca ggttaaatcg ggtgatgcga ttgtcattat gtctaatggc ggttttggtg
     9061 gcattcatgg caagttagta aatgcgttaa gggcaaagtt tgcttaatga aaaaaa
//
LOCUS       DAOQMB010000048         9019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3020_length_9019_cov_15.305556, whole genome shotgun sequence.
ACCESSION   DAOQMB010000048 DAOQMB010000000
VERSION     DAOQMB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9019
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3020_length_9019_cov_15.305556"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLY05_03145"
     CDS             <1..330
                     /locus_tag="PLY05_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC02329.1"
                     /translation="HAYFAGDTGYAPHFSDIYQRLGAPRVALLPIGAYLPRELMRQVH
                     MNPADAVQAHLDLKARLSLGIHFNTFQLTDETIDAPLADLKKSLWQQQVPEQDFIVLL
                     EGEGREV"
     gene            complement(391..>4767)
                     /locus_tag="PLY05_03150"
     CDS             complement(391..>4767)
                     /locus_tag="PLY05_03150"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HNC02330.1"
                     /translation="LFPGSKSFDASVLEFKFTVTDANLTQVTFDILFGSDEYPEYVGQ
                     FVDIAAVYVDGINYAFFENGKPLSVIQDNLTPGNFYNNKDRALAIEYDGMSAPLKITG
                     NLAAGTTHKIRIAIADTKDSVLDSGIFISNLQAVADTGETGVRLNNSDILDGGLGADT
                     MKAGYGNDFYSVDNVGDVIEEDDGKGNDTVNSTINYTLSANLENLNLLETQNGDNNLT
                     GEGNAKNNRLKGNSGNNLLVGNAGNDTLDGGLGTDTLVGGEGDDSYIIRDESDVIIES
                     GLSTNDIVKSTVSFTLSNNLEHLILLDNALNGTGNTGNNRVTGNKLNNILTGDAGNDT
                     LDGSEGDDTLIGGTGDDTYYIDSAKDIINELIGQGKDTVFSSISYVLAANIENITLTG
                     DANLDATGNDINNIITGNNGKNILTGGKGNDIYIIGEGDTVIELENEGIDSIQSSVSY
                     NLSNSHIENLSLLGKDNIDAIGNHLNNTLTGNEGNNILNGGQGVDLMIGGLGDDIYYI
                     DSINDKITELSNQGNDTVISTISYSLANTELEHITLIGKEHLKATGNSKNNILIGNEG
                     NNTIDGGTGNDTLTGGLGDDTYIIDNASDVIVELANQGNDTVFSQVSYSLGGTSIENL
                     RLIGTGTLNATGNDENNLIVGNNANNDLSGGKGSDTLEAGAGNDTLRGGDGADFLLGG
                     GGNDTYYIENQDDFIKELPLIGTDTVISTLNYTLGAYLENLTLEGSATSGDGNELDNN
                     IVGNTNDNILNGYRGNDTLDGGVGADTLIGGIGNDTYIIDNINDVITEFDKQGIDTVQ
                     SKITYSIASLEFIENLTLLGFGDLSATGNKLNNIIKGNDAKNTLDGGEGIDTLLGGNG
                     DDTYIVDNLNDQVIEVADNGTDTIQTNLSYILTDNIENLILTGTSNITGTGNTLSNKL
                     IGNAHDNILNGLSGNDTLEGGAGNDSYIFTRNSGMDEIFDNLGNNTLLINNLLLSELG
                     FSKASNNLEVSINGRSDKVIIRDWYTNNNNRMSLQLTDGTTLSATQLEEKANANTSNN
                     GSGAGSSGNDNLTGTTGNDNLNGGEGADTMAGGSGDDIYTVDNTGDVVIENENQGTDT
                     IKSSVNYTLSNHVEKLVLTGTGFIKGTGNTLNNYITGNSGNNILDGGSGIDTLEGGAG
                     DDIYIVDHKDDLINEIAGEGYDTVRSFIDYTLGANIEVLSLIDALALKGTGNQLNNLI
                     NGNDANNTLTGGDGKDTLKGGLGDDTYIIQATDIADIITEKTNEGNDTIQSYATYELS
                     DNVENIILLGNNAIDAYGNSQNNTLIGNDANNTLNGRAGNDTLQGGLGNDVYVFGRGA
                     GQDLINNTDANNGNDKVLFNTDVNYQQLWLERKDNHLVLTISAYQEIFTEETINGTTS
                     LVAKVKLSKTNDTVTIQDWYSNSAKQVDSFELSDGKVLLASEVQQLVDKMASFGVFKD
                     GSLTVNKNQYDSDILPVLNITW"
     assembly_gap    4768..4867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4868..5374)
                     /locus_tag="PLY05_03155"
     CDS             complement(<4868..5374)
                     /locus_tag="PLY05_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC02331.1"
                     /translation="MATINGTNNNEMLLGTNGDDTLYGLAGNDTLFGLEGNDTLDGGT
                     GIDSLSGDLGDDQYSVDDTNDIVVELINQGKDSVLSTVTYTLSANLENLTLLGNLAIN
                     GTGNDSANLILGNSANNILVGGGGFDTIEGGSGDDSITGGGTTDKLYGQDGNDTLTGG
                     GGSDELYGG"
     gene            complement(5737..6144)
                     /locus_tag="PLY05_03160"
     CDS             complement(5737..6144)
                     /locus_tag="PLY05_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HNC02332.1"
                     /translation="MLTGSCLCGGIHYQYAGDITEIALCHCQQCRKAQGSAFASNSPI
                     DSRLLTFTQGEELLQSYYSSPLKKRVFCQRCGSPLYSQRDDLPEIIRLRLGTLESPIS
                     CQPSYHIYAASKAEWWTINDDKPQYSKLKPREN"
     assembly_gap    6148..6247
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6575..7783
                     /locus_tag="PLY05_03165"
     CDS             6575..7783
                     /locus_tag="PLY05_03165"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNC02333.1"
                     /translation="MPVQLVLNANEKNGHKPVKIFTNEVDPQALQQLRNLAALPFVYS
                     HIAAMPDVHVGIGATVGSVIPTKGAIIPAAVGVDIGCGMNAVRLSLKAHQLPDNPKVL
                     RTAIEKAVPVGFAEHKKETVKASSISALDVGIDKIVGKHAGIMKMIKQFRQTWARQLG
                     TLGGGNHFIEICLDENQDVWVMLHSGSRGIGNCIGRYFIELAKKDMHREYGHLPDKDL
                     SYLVEGTQHFADYVEAVSWAQDYAMLNRREMMRLVVEALKTVLPSFELTKEAINCHHN
                     YVEQETHFGEQVYITRKGAISAQEGQLGIIPGSMGAKSYIVRGKGNGESFCSCSHGAG
                     RKMSRMEAKRKFDSHDLVLQTEGIECRKDKGVVDEIPAAYKDIEAVMAYQSDLVEVVH
                     TLRQIVCIKG"
     gene            7904..8845
                     /locus_tag="PLY05_03170"
     CDS             7904..8845
                     /locus_tag="PLY05_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02334.1"
                     /translation="MKQSQLDEVIACLPKEKTCFYYHRDRYVLYLLGRLQQHGGMRTI
                     RDVKKSRYAHWLNKPLIKDWLARCGRDDIALAELLHYWPQQSIHNYVLNLAQWGNEQW
                     NPWYQTSRAGLNAVLQLNLPISHVKNFRDQGVGQVWRYGRWHHPMSEQRITLAWARLD
                     WDWQTGQAMIEEIQSDWLRDYAELHAAAKQAKQEHEDRVWWRNGWLPVDVVLDYQMDI
                     QQHQKTWSEAMLTAALWFLQEELGFKEVFYHAWQTGNALKRLDDEHIPPRSLYSDLPE
                     KFAFERVNKGPLFLESDPKVKKVKKRQDIWQWYRWAA"
BASE COUNT         2600 a         1725 c         1681 g         2813 t
ORIGIN      
        1 catgcctatt ttgcaggcga tacaggttat gcgcctcatt ttagcgacat ttatcagcgt
       61 ttaggtgcgc cacgagtcgc cttattgcca attggtgctt atttgccaag agagttaatg
      121 cgtcaagtac atatgaatcc tgctgatgca gtacaagcgc atcttgattt aaaagcacgg
      181 ctgtcgttag gtattcactt taataccttt caattaaccg atgaaacgat tgatgcgcca
      241 ttagccgatt taaaaaaatc attgtggcaa caacaagtgc ctgaacagga ttttattgtg
      301 ttattagaag gtgaaggtag agaggtgtaa tgtctaaaaa gaaacaaacc caaaaaccac
      361 ctaaagtggc ttttgagttt attgacaaga ttaccaagtg atgtttaaaa caggtaagat
      421 atcagagtca tactgatttt tattaacagt caagctacca tccttgaata caccaaaact
      481 agccatttta tctactaatt gttgaacttc tgaggcaagc aatactttgc catcgctcaa
      541 ttcaaaacta tcaacttgtt tagcggagtt gctataccaa tcctgaattg taacagtatc
      601 atttgtttta ctaagtttca cttttgcaac caatgatgtg gtgccattaa tagtttcttc
      661 agtaaaaatt tcttgatagg cactaatggt aagaacaaga tgattatctt tacgttctaa
      721 ccataattgt tggtaattga catctgtgtt aaataagact ttgtcattac cattattagc
      781 atcagtgttg tttattaagt cttgacctgc accacgacca aacacataga catcattgcc
      841 taagccacct tgtaatgtat cattaccagc tctaccattg agtgtattat tggcatcgtt
      901 acctattaat gtgttgtttt ggctattgcc ataagcatca atagcattat ttcctagtaa
      961 gatgatgttt tcaacgttgt cagaaagctc ataagttgca tagctctgaa tggtatcatt
     1021 accttcattg gttttttcgg taataatatc tgcaatatca gttgcctgaa tgatataagt
     1081 gtcatcacct aaaccgcctt taagtgtgtc tttaccatca ccacctgtta aggtgttgtt
     1141 ggcatcatta ccattgataa ggttattaag ttgattgcct gtccctttga gtgctaaagc
     1201 atcaattaag cttaagactt caatatttgc acctaatgta taatcaataa aacttctaac
     1261 agtatcgtaa ccttcgcctg caatttcatt aattaaatca tctttgtgat caacaatata
     1321 aatatcatcg ccagcaccac cttcaagggt gtcaatgcct gaaccaccat ctaaaatatt
     1381 attgccgcta ttaccagtta tgtaattatt gagagtatta cctgtgcctt tgataaagcc
     1441 agtacctgtt aataccagtt tctcaacatg gttagacaat gtgtagttga cagagctttt
     1501 aatggtatct gtgccttgat tttcgttttc aataacaaca tcgccagtat tgtcgactgt
     1561 ataaatgtca tcaccactac caccagccat ggtatcagca ccttcaccgc cattgagatt
     1621 atcattgcct gtagtgcctg ttaaattgtc gttaccactg ctaccagctc cagagccatt
     1681 attagaagta ttagcattgg ctttttcttc taattgggtt gctgatagag tagtgccatc
     1741 tgttaattgt aaactcatac gattattatt attcgtgtac caatctctga tgataacttt
     1801 gtcactgcgc ccattaatac tcacttctaa gttattgctt gctttgctaa agcctagctc
     1861 actgaggagt aaattgttga ttagaagagt attgttacca aggttgtcaa atatttcatc
     1921 catgccgcta ttacgtgtga aaatatagct atcattgcca gcaccacctt ctagagtatc
     1981 attgccactt aatccattta gaatattgtc atgagcattg ccaattaatt tattgctgag
     2041 cgtgttacct gttcctgtaa tattacttgt acctgttaat atcagatttt caatattgtc
     2101 agtgagtata taggaaagat ttgtttgtat agtatcagtg ccattatctg ctacttcgat
     2161 aacttgatca ttcaaattat caacaatata cgtatcatca ccgtttccgc caagaagggt
     2221 atcaataccc tcaccaccat ctaaggtgtt tttagcgtca ttacctttaa taatattgtt
     2281 gagcttattg cctgtcgcac ttaaatcacc aaaacccaat aaggtcagat tttctatgaa
     2341 ttccagagag gcaatagagt aggtgatttt actttgaacg gtatctatac cttgtttatc
     2401 aaactctgta atcacatcat ttatgttgtc aataatataa gtatcattac cgataccacc
     2461 aataagagtg tctgctccca caccgccatc taaagtatca ttgccccgat atccattgag
     2521 gatattgtcg tttgtatttc ctacaatatt attgtcaagc tcattaccgt caccacttgt
     2581 tgcagagccc tctaatgtta agttttctaa ataagcacca agtgtataat ttaacgtgct
     2641 aatcactgta tcagtaccaa ttaaaggcag ttccttaata aaatcatcct gattctcaat
     2701 ataataagta tcgttaccac cgcctcctaa taagaagtct gcaccgtcgc cacctcttaa
     2761 tgtatcatta ccagctcctg cttcaagcgt atcactacct ttgccaccag aaagatcatt
     2821 attagcatta ttcccaacga ttagattatt ttcatcattg cctgtagcat tgagtgtgcc
     2881 agtgccaatt aaacgaaggt tttcaatact agtaccacct aatgaatagg aaacttggct
     2941 aaatacagta tcattacctt gattagctaa ttcaacaatc acatcactag cattgtcaat
     3001 aatataagta tcatctccta atccacctgt gagggtatca ttacctgtac caccatcaat
     3061 agtgttattg ccctcattgc caattagtat attatttttg ctatttcctg tagctttaag
     3121 atgttctttg cctattaatg taatatgctc tagttcagta ttggccaaag aatatgagat
     3181 tgtactgata acagtatcgt tgccttgatt tgacagttcg gtgattttat cgttaatgga
     3241 gtcgatgtaa tagatatcat cgcccaaacc accaatcatc aaatcaacgc cttgcccgcc
     3301 atttaatata ttatttcctt cattacctgt taaagtgtta tttaagtggt ttcctatagc
     3361 atcaatatta tctttgccta gtaggctgag attctcaata tgactattgc ttaaattata
     3421 acttacagag ctttgaatac tgtcaatgcc ttcattttca agttcaataa ctgtgtcacc
     3481 ttcaccaata atatagatat cattaccttt tccaccagtg agaatgtttt tgccgttatt
     3541 gcctgtaata atattattaa tatcatttcc tgtagcatca aggtttgcat cacctgttag
     3601 ggtgatattt tctatgttgg cggctaacac ataactgata ctgctaaaaa cagtatcttt
     3661 accttggcct attaattcat tgatgatatc tttagcggag tcaatatagt atgtgtcgtc
     3721 acctgttcca ccaattaaag tatcatcacc ctcactacca tcaagtgtgt cgtttcctgc
     3781 atcacctgtt aatatattgt tgagtttatt gcccgtaacg cggttgttac ctgtgttacc
     3841 tgtcccattt aaggcattat ctaataatat taaatgttcg agattatttg ataaggtgaa
     3901 agaaacggta ctttttacga tatcatttgt tgataagcca ctttcaatga tcacgtcact
     3961 ttcatcacga ataatataac tatcgtcgcc ttctccgcca actaatgtgt cagtacctaa
     4021 tccgccatca agggtatcat ttcctgcatt accaactaat aggttattgc cactattgcc
     4081 ttttaagcgg ttgtttttag cattgccttc accagttaga ttgttatcac cattttgtgt
     4141 ttctaataag ttgaggtttt ctaaatttgc agaaagggtg taattaatgg tgctattaac
     4201 ggtgtcatta cctttgccat catcttcttc aataacatca ccaacattat caacagaata
     4261 gaagtcatta ccataacccg ctttcatggt atcggcacct aggccaccat ctaaaatatc
     4321 gctattgttt aggcgtaccc cagtttcacc tgtatcagca actgcttgta aattagaaat
     4381 aaaaataccc gagtccaata ccgaatcttt ggtgtcggca atggctattc taattttgtg
     4441 tgttgtccct gcggctaagt tgcctgtaat tttaagcgga gcagacatac catcatattc
     4501 gatagctaaa gcacgatctt tgttgttgta aaagttgcca ggcgttaagt tatcttgaat
     4561 aacacttaat ggtttaccat tctcgaagaa ggcatagtta ataccatcaa catataccgc
     4621 agctatatcg acaaattgtc caacatattc aggatattcg tcagagccaa ataaaatatc
     4681 aaacgtaact tgggttaaat ttgcatcagt aacagtaaat ttaaactcta aaactgaagc
     4741 atcaaaactt tttgagcctg gaaaaagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnngcc accatatagc tcatctgagc caccgccacc tgttagtgtg tcattgccat
     4921 cttgtccata aagtttgtca gttgttccac cgcctgtgat gctatcatct ccagagccgc
     4981 cttcaatggt atcgaatcca ccgccaccga ctaaaatatt gttagcactg ttgcctagta
     5041 tcaaattggc agagtcatta cccgtgccat taatggctaa attacccaat aatgtgaggt
     5101 tctcaaggtt agcagataag gtataagtca cggttgataa aacggagtct ttaccctgat
     5161 taatcaattc cacaacaatg tcattagtat catcaacaga atattggtcg tcacctaaat
     5221 caccagataa tgagtctata ccagtgccac catcaagcgt gtcattgcct tcgagtccaa
     5281 atagagtgtc attgcctgct aagccataaa gggtatcgtc accatttgta ccaagtagca
     5341 tttcgttatt gtttgtgccg ttaattgtag ccatgatgag aattctccat gtggacaggc
     5401 tgtgttagca aactgattct tttactttta atgaatgcac tatctgatgt tgagtacttc
     5461 atttatatct aattataaat ctacaacaat cttaatttga tctctagtga ttaatgttta
     5521 attggtttgt tgtaccgata agatgaccaa gtaagatttt atatcatgct tttagcgatg
     5581 tacgcatcga aaaagtcaaa gctcataaat ctatatctct acttatatta aagggttttt
     5641 aaataaaaat atgctgtttt ttgtcactct gtgaggcaat atgaagactt ttatcgaggc
     5701 aaaggggtta ttaagtcgtc tccttgacaa aaagccttaa ttttcacgag gttttagttt
     5761 ggaatattga ggtttatcat cattaattgt ccaccattca gccttacttg ccgcataaat
     5821 atggtaactg ggctggcaac taataggact ctctaaagta cctaaacgta aacgaataat
     5881 ctctggtaaa tcatctcgtt ggctatagag tggactgcca caacgttgac aaaaaactcg
     5941 tttttttaat gggctagaat agtagctttg taatagctct tcaccttggg tgaaggtcaa
     6001 taaacggctg tcaattggcg aattactcgc aaaggcagag ccttgggctt ttcggcattg
     6061 ttggcaatga cataaggcaa tttctgtaat gtcacctgca tattgatagt gaatcccacc
     6121 acataaacaa ctacctgtta gcataatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnngct gttaaaaaat cttttgtcag atatattgat tatttacata agaaatacta
     6301 atatgcgcgc cattcctaat cagacactaa ttttggaggt tcttgaacaa tgagtcgtat
     6361 tcagtattta ccatttgctt aatacacttc ttcaagaatc agagcttgaa ggagtgataa
     6421 cccttcaagc tagagaatta aaagccctag ctaaccatgc tagggctttt tctttgggca
     6481 gtcatttttg ttatttaccc tataacaaaa tcggctgtaa ggattaccca taaaaagcac
     6541 aaagtcatcc tttgtgtttt tagagagatt tatcatgcct gtacaattgg ttttaaatgc
     6601 caacgaaaaa aatggtcaca aacccgttaa aatttttact aacgaggttg acccacaagc
     6661 tttacaacaa ttacgcaacc tagccgcgtt gccctttgtt tattcgcata ttgcggccat
     6721 gcctgatgtc catgttggca ttggtgcaac cgttggctcg gtgattccga ccaaaggtgc
     6781 aattattcct gcggcagtgg gtgtggatat tggttgtggc atgaatgctg tgcgcttgtc
     6841 actcaaagcc catcaactac ctgataaccc caaagtattg cgtacggcaa ttgaaaaagc
     6901 tgtgcctgtg ggttttgctg aacataaaaa agaaacagtc aaagcttcta gtatcagtgc
     6961 cttagatgtg ggcattgata aaattgttgg taaacatgca ggtatcatga aaatgatcaa
     7021 gcagtttcgc caaacatggg cacgacagtt agggacattg ggtggcggta atcattttat
     7081 cgaaatctgt ttagatgaaa accaagatgt ttgggtgatg ctacattctg gtagtcgagg
     7141 cattggcaac tgtattggtc gatattttat tgagttggcc aaaaaggata tgcaccgtga
     7201 atatggccat ttgcctgata aagatttgtc ttacttggtg gaaggtacac aacattttgc
     7261 ggactatgtt gaagcggtct cttgggcaca agattatgcc atgctcaatc gtcgggaaat
     7321 gatgcgttta gtggtggaag ccctgaaaac agtattaccg agttttgagc tcactaaaga
     7381 agcgattaat tgtcatcata attatgttga acaagaaacg cattttggtg agcaggttta
     7441 tattacccgc aagggcgcaa ttagtgcaca agaaggtcag ttaggcatta ttccgggcag
     7501 tatgggcgca aagtcctata ttgtccgagg taaaggtaat ggtgagtcat tttgttcttg
     7561 ttcgcatgga gcagggcgca aaatgagtcg aatggaagct aaacgcaaat ttgatagtca
     7621 tgacttggtg ttacaaactg agggcattga atgtcgtaaa gacaaagggg tagtggatga
     7681 aatccccgcg gcttataaag acattgaagc tgttatggcg tatcagagcg atttagtgga
     7741 ggtcgttcat accttacgcc aaattgtgtg tattaaaggg tgaggcggac taaggaaatg
     7801 tctgggagac atttactccg ccgaacgaca cgcaaggatg cgtggctgga tagaccctac
     7861 agggacgttg tgtttacacc gatttaaaca ggaggaatga ataatgaaac aaagccaact
     7921 agacgaagtc attgcttgtt tacctaaaga gaaaacttgt ttttactacc accgtgaccg
     7981 ttatgtgttg tacttgttag ggcggctgca acaacatggc ggtatgcgga ctattcggga
     8041 tgtaaagaag agccgctatg ctcattggtt aaataaacct ttgattaagg actggttggc
     8101 gcgttgtggt cgtgatgaca ttgctttagc agagttattg cattattggc cacaacagtc
     8161 cattcataat tatgtgttaa atcttgcaca atggggtaac gaacagtgga acccttggta
     8221 tcaaaccagt cgtgcaggac tcaatgccgt attgcaactt aatttaccta tttctcatgt
     8281 aaaaaatttt cgagaccaag gtgttggtca agtatggcgt tatggccgtt ggcatcatcc
     8341 tatgtctgag caacgtatta ccttggcatg ggcgcgtttg gattgggatt ggcaaacagg
     8401 ccaagcaatg attgaagaga ttcagtcgga ttggttacgt gattatgctg agttacatgc
     8461 agcagctaaa caggccaaac aagaacacga agaccgtgtt tggtggcgta atggttggtt
     8521 acctgtggat gtggtgcttg attatcaaat ggatattcaa caacatcaaa agacatggag
     8581 tgaggctatg ctgactgctg cattatggtt tttacaagaa gaattaggat ttaaagaagt
     8641 gttttatcat gcgtggcaaa cgggaaatgc cttaaaacgt ttggatgatg aacatattcc
     8701 ccctcgttct ttatatagtg atttgcccga aaaattcgcc tttgagcgag tgaataaagg
     8761 gccgttattt ttagaatctg acccgaaagt aaaaaaggtc aaaaaacgcc aagatatttg
     8821 gcaatggtat cgctgggcag cttaggagat agaaaaatgc ctaaacgaca tgcagtcaaa
     8881 ctgcaaaaaa gaccgcgtaa tttagtgact ttggttgcga ttgcgcaaaa agggggtgct
     8941 catggtaaaa gtgaaaaagc gttacgccgc accgaacgtc aacacacaca aatgtccgca
     9001 cgggaggctt tttcctccc
//
LOCUS       DAOQMB010000049         8924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3064_length_8924_cov_14.498816, whole genome shotgun sequence.
ACCESSION   DAOQMB010000049 DAOQMB010000000
VERSION     DAOQMB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8924
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3064_length_8924_cov_14.498816"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLY05_03175"
     CDS             <1..426
                     /locus_tag="PLY05_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02335.1"
                     /translation="SVTQDEIIKAAELGIIEFIEEGSRVKVPSPRLLDAGLKLVSLGL
                     PLHELLNIVGGLRGNVERVANMFVDVIARLIDTYGKENIPPSSATTRLADLIWQMRPL
                     ADIAIDAEVARAMEKAIAKYFGERLDAIMEHLKNKDGNV"
     gene            658..2880
                     /gene="rbtA"
                     /locus_tag="PLY05_03180"
     CDS             658..2880
                     /gene="rbtA"
                     /locus_tag="PLY05_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhombotarget A"
                     /protein_id="HNC02336.1"
                     /translation="MPASQSRVLLLSLLTGSLITTATWADTVVVNTTTDENNIDNTSC
                     SIREAVSYLKAKNDKKAIIDEEIAVIAGTSLKNSSQLTIAQNALTDEKAKTSPNASEI
                     SRLEKEIADLTNVINNGLFSLNIQLAQKTDELNKEKSKSTPDNNLIASYDAAINTLKD
                     QIKQKETERTQKESDLLVYRLKGLYGCISFDFNTADNVSLYANLGTYSIDSTITLPFS
                     LNFALFGADASSSAVGIINLEDTTNPDALPRTILKATASHNIFIIDDGKDNDHDSNPA
                     TSNQYITVTFNNIDFLGCDNNCATNGGIILNKEFLTITNSVIAKGVASEKGGAIYNAA
                     NSVLSIKNSVIKNNRAESGSAIFSEDANLALSSSLITDNETTNINNGVITLNKTLKAS
                     PYAPINPLIENSTISGNKGIGLNIQEGIFVNTSTVVQNTVGISFNSGLPTVYNSIIAD
                     NSTLDCQSFAPIPADNKAYFANNLSVKDKGCPTTNVFNSLLISNTGAETLMAATDSKG
                     HCVAPPAVGLLCPLANNGGLTRTHKPRLLASYTKLNDSVIVNKGFNINKNSAGLACSS
                     LDQRGKPRENNENACDIGAVELISGLSSSRQGDDIVFGQIKRFSPLENLGDAELLPAE
                     LCANFLGAGEYINGCIRVIDLPRHGQASIDTQTGELLYSTTKTDFHGFDTFSYGVITT
                     LSRFNDANNDRTLTTSVRVVSEPPSSPPSKALDNGANGIFSILMLSLVAIWRRIYRGQ
                     "
     gene            3133..5025
                     /locus_tag="PLY05_03185"
     CDS             3133..5025
                     /locus_tag="PLY05_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03501.1"
                     /note="This protein contains a GlyGly-CTERM
                     protein-sorting domain, as detected by TIGR03501. These
                     domains are found at the C-terminus of secreted proteins
                     in organisms that possess both rhombosortase, which is an
                     intramembrane serine proteinase (see TIGR03902), and a
                     type II secretion system (T2SS). In at least some cases,
                     such as VesB from Vibrio cholerae, cleavage by
                     rhombosortase is followed first by attachment of a
                     glycerophosphoethanolamine-containing moiety, then by
                     transport by the T2SS across the outer membrane and
                     release into the medium in soluble form; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlyGly-CTERM sorting domain-containing protein"
                     /protein_id="HNC02337.1"
                     /translation="MVKEAMVILGANKNDQVDNITQTKPKRTAATTTINAQQKNRIFS
                     TATSKAALTLNHLKLVNGYHNEYGGAIVAGGLLDLFNVVFENNKADKMGGAVHLLGNA
                     SDLSANNSTWQQNLANGGVGSALSMTCLADLKPTARNLVIQNSSFINNGSGNDQSILD
                     ICGNLTFNLQSSTIAQNKINNSGNIINFNENTSPLSTVSFLNATIINNEGGSALFFGK
                     LGTIQINHSILSFNQAKSCHSNLASSDINYSGDNNLYQGCDILKVASTTSTNAHPNDQ
                     HITFGSINWTELFNPLGNYGGYTQVYLPKQNALSKQYVVDKITSGACQDYIDQRGSNT
                     HSTSNVENCDRGSVERRAAIAVVDRTALFTNKDKTDRIVEVNVLDNDIPSETDLTDDQ
                     PNARGSFAKDAEGNYQLKLTNDSGGLCTIRHRTSENLLPYVKFDNGGKLINQLQSGSC
                     KYSFIDSNGNQAIEGQLFFTIENKLPIAGDDTFYLQAGASQITMNLTTNDNDDNDGKY
                     GGLCTSNTVKCNGGYYIRVVDSPKIGTIEGESRPCPDYTDTNKNICYRGDLIYQSKND
                     LAPFNDKFTYVVYDNDLAFSAPATVTIISENAEKANDTGGSLAWWSLLSLTGLALYRR
                     RNIKIA"
     gene            5018..5626
                     /locus_tag="PLY05_03190"
     CDS             5018..5626
                     /locus_tag="PLY05_03190"
                     /inference="COORDINATES: protein motif:HMM:NF021358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HNC02338.1"
                     /translation="MPKTISHLLISGSLIAATAVYAQENTVTLDVPEVVHILSINDQE
                     QNSTFFGSRQHIKKLNVGENTLTVRYSQLFNLTADEHDIIKSKPLIIRFTAEAGKSYQ
                     LLASPPKRYEAAKEFAKNPDIRLVDKNTGTSQTAVLVKSYTNASLVDTIGKAFQNSSD
                     ENQAVIPSSNNAQLLQELWLKASPQEREAFAAWLATKATNAK"
     gene            complement(5766..7187)
                     /gene="creC"
                     /locus_tag="PLY05_03195"
     CDS             complement(5766..7187)
                     /gene="creC"
                     /locus_tag="PLY05_03195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF008312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HNC02339.1"
                     /translation="MSIFLRIWLVFSLVLVVGAYLTIKTLQEEIKPSVRQAIEDTLAD
                     NANIIAALVADDVKTGKVDTPEFREKIIEVLQRPLRAQIWQYQKENVAQHIYITNDKG
                     IVLFDSQGIATGKDYSRWNDVRRTLDGKYGARTTRQDPKNELTSTMFVAAPIIDQQKI
                     IGVVSVSKKSQLLQPFIERAQEQMLKQGLWVVVLALILSGGVAWWLRHGIYQVAEYAR
                     TLSTQRQTNLHFWAAKELNSLVYAIDKMRQELEGKNYIERYVHTLTHELKSPLTAIKA
                     SAEILQDDLDETDRQRFSTNIIEQTDKLQLLIERLLLLARLEQQQQMNQQQIDLSLLV
                     EKVIQQKQAVISKKQIQITNHLPTTLLIRGEVFWLEQAISNIIDNALDFTPYAHAISL
                     NAQQHHSSLSLSIENQGQQIPEYALEQVFERYYSLPRPDSQKKSTGIGLTLVKEVMNL
                     HGGKVSIQNHQDGVRVILVFLKS"
     gene            complement(7187..7906)
                     /gene="creB"
                     /locus_tag="PLY05_03200"
     CDS             complement(7187..7906)
                     /gene="creB"
                     /locus_tag="PLY05_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004958414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HNC02340.1"
                     /translation="MANTVFFREILAVLKHILLVEDDEAIAQTLIFSLQREGWQVQWC
                     ALGQQALDYVAQHPVDFIILDVGLPDINGFDLCRQIRQQYQTPLLFLTARNDEVERII
                     GLEIGADDYCAKPFSPRELVSRIRVICRRLQPQATPIHSNSLAKIWQHDPEKLQISYH
                     GQLLNLTRYEYRLLEVLLKQPERVLSRHQLMQAAWEHPDHSLERTIDTHIKVLRQKLK
                     NIHDDEVIKTHRGIGYSLAKY"
     gene            complement(7948..>8924)
                     /locus_tag="PLY05_03205"
     CDS             complement(7948..>8924)
                     /locus_tag="PLY05_03205"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC02341.1"
                     /translation="IYEGEYLLGTSIARPLIAKRLVEIAQEQGADAISHGATGKGNDQ
                     VRFELGAYALTPNIRVIAPWREWDLTSRETLMNYAAKHNIPIDFNKAGKKSPYSMDAN
                     LLHISYEGGGLEDPFWEPEEDIWRWTVSPEKAPDQPEYVELTYRGGDIVAINGTEMTP
                     AQVLTQLNALGGKHGIGRLDLVENRYVGMKSRGCYETPGGTIMMKAHRAIESITLDRE
                     VAHLKDELMPRYASLVYNGYWWSPERQMLQTMIDESQRYVNGVVRVKLYKGNVYIAGR
                     KSDDSIFDANIATFEDDRGAYNQKDAEGFIRLNGLRLRIAAGKGRKLL"
BASE COUNT         2863 a         1704 c         1545 g         2812 t
ORIGIN      
        1 agtgtgacac aagatgaaat tattaaggct gccgagctgg gcattattga atttatcgaa
       61 gaaggctcaa gagttaaagt acctagccct cgtttattag atgcgggact caaattagtc
      121 tctttgggct taccattgca tgagttactc aatattgtgg gtggtttacg tggcaacgtt
      181 gagcgtgtag caaatatgtt tgttgatgtt attgcacgtt taattgatac ttatggtaaa
      241 gaaaatattc cgccaagcag tgccactacc cgtcttgcgg atttaatttg gcaaatgcgg
      301 ccattagccg acatcgctat tgatgccgaa gttgcccgtg ccatggaaaa agcgattgct
      361 aaatactttg gcgaacgttt agatgctatt atggagcatc taaaaaataa agatgggaac
      421 gtataacaac agacatcata gacaatacga caaaataaca atttttcctg aaattgcgcg
      481 attgtctatg ctacagtcgt atataattac gccccatgca acacaaacac acaagaacaa
      541 atcattattt ttaagtacaa tgtttgacaa aattacaaat atgcttttct catagcaaat
      601 gtaaaaataa tttttgttct taataaaaaa taacaagcta gtttggagac tcttccgatg
      661 cctgcttctc agagccgcgt tttacttctt agtttactaa cagggtcatt aataacaaca
      721 gccacttggg ctgacaccgt tgttgttaac accacaactg atgaaaataa tattgataat
      781 acaagttgtt ctattcggga ggctgtaagt tatctaaaag ccaaaaatga taagaaggca
      841 atcattgatg aagaaatagc cgttattgcg ggtacatcgc ttaaaaatag cagtcaactc
      901 acaatagccc aaaatgcttt gactgacgaa aaagcaaaaa catctcctaa cgcatcagag
      961 ataagtcgat tagaaaaaga aattgctgat ttaactaatg ttataaataa cggtttattt
     1021 tctctaaata tacaactagc tcaaaaaacc gatgaactca ataaagaaaa aagtaaatca
     1081 acacctgata ataatttaat tgctagttat gatgcagcga ttaatacatt aaaggatcag
     1141 attaaacaaa aggaaactga acgcacacaa aaggaatccg atcttttagt atatcgcctt
     1201 aagggattat atggctgcat tagttttgat tttaataccg ctgataatgt ttctttatat
     1261 gcaaatttag gtacttattc tattgattca accattacac ttccatttag tcttaatttt
     1321 gctttatttg gtgcagatgc ttcatcgagt gctgttggaa ttataaatct agaagatact
     1381 actaatcctg atgccttgcc tcgcactatt cttaaagcaa ctgctagtca caatatattt
     1441 atcattgatg atggcaaaga taatgaccat gacagtaatc ctgctacatc aaatcaatat
     1501 atcaccgtca catttaacaa tatagatttt ttaggttgtg ataataattg tgccactaac
     1561 ggtggaataa ttttaaataa agagtttttg accattacta acagtgttat tgcaaaaggt
     1621 gttgctagcg aaaaaggtgg tgctatttat aatgcggcaa actccgtatt atcgataaaa
     1681 aactctgtta tcaaaaataa tcgggcagaa tctggctctg ctattttttc agaagatgct
     1741 aacttagctt tatcaagctc acttattact gacaatgaaa caacaaacat taataatggt
     1801 gtcattacac ttaataaaac tttaaaagca tcaccctatg cacctataaa ccccttaatc
     1861 gaaaatagca caatttcggg taacaaaggc ataggtttaa acattcaaga aggtattttt
     1921 gttaatactt caacagtcgt gcaaaatact gttggtatta gttttaattc tggcctgcct
     1981 accgtttata atagcattat tgccgataat agtaccttag attgccaatc atttgcccct
     2041 attcctgctg ataataaagc ctattttgcc aataatttgt cagtcaaaga taaaggctgt
     2101 ccaacaacaa atgtttttaa tagcctctta attagcaata ctggtgcaga aactttaatg
     2161 gctgcaacag acagtaaagg tcattgtgtt gctcctcctg ctgttggttt attatgccca
     2221 ttagccaata atggtggttt aacacgtacc cataaacctc gtttattagc aagttatacc
     2281 aaactgaatg actccgtcat tgtcaataaa ggctttaata tcaataaaaa tagtgctggc
     2341 ttggcttgct ctagcctaga tcaacgaggc aaacctcgtg aaaataacga aaatgcctgt
     2401 gatattggtg ctgttgagtt aatttctggc ttatctagca gtcgtcaagg tgacgatatt
     2461 gtttttggtc aaatcaaacg ttttagccca ttagaaaact taggtgatgc agaattactc
     2521 cctgccgaac tttgtgctaa ttttttaggt gctggtgaat atattaatgg ctgtattcgc
     2581 gtcattgatt tacctcgaca cggccaagcg agcatagaca ctcaaacagg cgaattatta
     2641 tactcgacaa caaaaacaga ttttcacggt tttgatacct ttagttatgg tgtcattaca
     2701 acattaagtc gttttaatga tgcgaacaac gatagaacct taaccacttc tgtacgagtt
     2761 gtatctgaac caccaagttc ccctccctcg aaagcattag ataatggtgc aaatggcata
     2821 ttttctattt taatgttgag cttagtggct atttggcgac gtatatacag aggacaataa
     2881 taaatgaaat ttcgtcaatc tcttttaaca ttgagtattt tgttaagtaa ttatgcttat
     2941 tctgctgtaa ccatcaatgt caacactaca aatgatgagt ttggcgaaaa tcttgcaaat
     3001 tgttcattac gcgaagctat tgaggccgtc aacacacgta atccttttgg cggatgcagg
     3061 tctggcgaac gttttggtac taaccgtatt gttttagaag ataacgaata tattttgacg
     3121 cgtggcgagt tagtggttaa agaagcaatg gttattttgg gtgcaaacaa aaatgatcaa
     3181 gttgataata ttacccaaac taaacccaaa cgtactgcag caacaaccac tattaatgct
     3241 caacaaaaaa atcgtatttt tagtaccgca acaagcaaag ctgctttaac actcaatcat
     3301 ttaaaattag ttaatggtta ccataatgaa tatggcgggg ctattgttgc aggcggctta
     3361 ttagacttat ttaatgttgt ctttgaaaat aataaagccg ataaaatggg aggagctgtt
     3421 catctactag gtaacgcttc agatctatca gccaacaata gcacttggca acaaaatctg
     3481 gctaatggtg gtgttggctc agcattaagc atgacttgcc ttgctgattt aaagccaaca
     3541 gcacgtaatc ttgtcattca aaatagtagt tttattaaca atggctcagg taatgatcaa
     3601 agcatactag atatttgtgg aaacttaact tttaatcttc aatcttctac gattgcacag
     3661 aataagatta acaatagtgg taatatcatt aacttcaatg aaaatacatc acccttaagt
     3721 actgttagtt ttcttaatgc cactattatc aataatgaag gtggttctgc attatttttt
     3781 ggtaaactcg gcaccatcca aattaatcat tctatattaa gctttaatca agctaagagc
     3841 tgccatagta atcttgcttc tagtgatatt aactatagtg gtgataataa tctttatcaa
     3901 ggttgcgata ttttaaaagt cgcgtctact actagcacga acgcacatcc taatgatcaa
     3961 catatcactt ttggtagtat aaattggaca gaactattta atcccttagg caattatggt
     4021 ggttatacac aggtttattt acccaagcaa aatgcactaa gcaaacaata tgttgtagat
     4081 aaaatcactt cgggtgcatg ccaagactat atcgatcagc gcggcagtaa tacacattct
     4141 actagtaatg tcgaaaattg cgatcgtggc tctgttgaaa gacgtgctgc tattgccgtt
     4201 gtagacagaa cagctttatt tacaaacaaa gataaaactg atcgtattgt tgaagtgaat
     4261 gttttagata atgatatacc tagcgaaaca gacttaactg atgatcaacc caatgctaga
     4321 ggctcttttg caaaagatgc tgagggtaat taccaattaa agttaactaa tgacagtggt
     4381 ggtttatgca ctattcgcca tcgtactagc gaaaatttat taccttatgt taaatttgat
     4441 aatggtggta agctaatcaa tcaattacaa agtggctcct gtaaatatag ctttattgat
     4501 agcaatggca atcaagccat tgagggacaa ctatttttca ctattgaaaa taaactcccc
     4561 atagcaggtg acgacacttt ttatcttcaa gctggtgcta gtcaaataac tatgaatttg
     4621 actaccaatg ataacgatga taacgacggt aaatatgggg ggttatgtac ctcaaacacc
     4681 gttaaatgta atggtggtta ctatatccgc gttgttgata gccctaaaat aggcacgatt
     4741 gaaggtgaaa gtcgcccttg ccctgactat acagatacta ataaaaatat ttgttatcgc
     4801 ggtgatttaa tatatcaatc gaaaaacgat cttgctcctt ttaatgataa attcacttat
     4861 gtcgtttatg ataatgatct tgccttttct gctcctgcaa ccgtcacaat tatcagtgaa
     4921 aatgcagaaa aagccaacga cacaggaggc agtcttgcat ggtggtctct actttcttta
     4981 actggtttag cattgtatcg tcgtaggaac attaaaattg cctaaaacta tttctcattt
     5041 attaattagt ggcagcttaa ttgctgccac tgctgtttat gcccaagaaa atactgtaac
     5101 acttgatgtt cctgaagtag tgcatatttt aagtattaat gatcaagaac aaaacagtac
     5161 attttttggc tctcgtcaac atatcaaaaa actaaatgtc ggtgaaaata cattaaccgt
     5221 acgctattct cagttgttta atttaacagc tgacgaacat gatattatca aatctaagcc
     5281 cttaatcatt cgttttactg ctgaagctgg caaaagttat caactactcg cctcgcctcc
     5341 taaacgttat gaagcagcaa aagaatttgc taaaaatcca gatattcgtt tagtcgataa
     5401 aaatacaggt accagccaaa cggctgtttt agtgaaatct tataccaacg cttctttagt
     5461 ggataccatt ggtaaagctt ttcaaaactc tagtgatgaa aatcaagcgg ttataccaag
     5521 ctctaataat gcccaacttt tacaagagtt atggttaaaa gccagtcctc aagaacgtga
     5581 agcttttgct gcatggttgg ctactaaagc aacaaacgct aaataatctg ttaacgcctc
     5641 gtatatttag gacttacgcg gttatcgctt atttgtttac acgtcaacca ttttcaacga
     5701 ttttatgttc acaaaacgcg ctaatttcgg tgaaatgcgt aagtccagat attatctatg
     5761 agggattaac ttttaagaaa taccaatatt accctcacac catcttgatg attttgaata
     5821 ctaactttac caccatgtaa attcatcact tctttgacta aggttaatcc gatacctgta
     5881 ctttttttct gactatctgg cctcggtaaa gaataataac gctcaaatac ttgttctaag
     5941 gcatattcag gaatttgttg gccttgattt tcaatactta aactcagtga tgaatgatgt
     6001 tgctgtgcat ttaagctaat tgcatgggcg taaggcgtaa aatctaaggc attatctata
     6061 atattagaaa tcgcttgctc taaccaaaaa acttcgcctc taattagtaa tgttgtaggt
     6121 aaatgattcg taatttgaat ctgttttttg ctaataaccg cttgcttttg ctgaataact
     6181 ttttccacca ataatgataa atcaatctgt tgctgattca tctgctgttg ttgctctaaa
     6241 cgagctaata ataataaacg ttcaattaat agctgtagct tatcggtttg ctcaataatg
     6301 ttagtactaa aacgttgtcg atctgtctca tctaagtcat cctgtaatat ttcagcactg
     6361 gctttaatgg ccgttaatgg gctttttagt tcatgggtta aggtatgtac ataacgctca
     6421 atatagtttt tcccctctaa ttcttgacgc atcttatcaa ttgcatagac taaactattg
     6481 agttcttttg cagcccaaaa atgcaaattt gtttgacgtt gtgttgataa agttcgcgca
     6541 tattctgcca cctgataaat gccatgacgc aaccaccaag caaccccacc acttaaaatc
     6601 aatgccaaaa ccacgaccca taaaccctgt tttaacattt gctcttgggc tcgttcgata
     6661 aaaggttgca acaattggct ttttttagaa acagaaacca caccaataat tttttgttgg
     6721 tcaataatgg gtgcagccac aaacatagtc gatgtgagtt catttttagg gtcttgacga
     6781 gtggtacgtg cgccgtattt tccgtctaaa gtacgtcgaa catcattcca tcgagaataa
     6841 tctttgcctg ttgctatgcc ttgtgagtca aataacacaa tccctttatc attggtaata
     6901 taaatatgtt gggcgacatt ttctttttga tattgccaaa tctgcgcacg caaaggccgt
     6961 tgtaaaacct ctattatttt ttctctaaac tcgggagtat caacttttcc tgttttgaca
     7021 tcatcagcta ctaaagcagc aatgatgttg gcattatcgg ctaaagtgtc ttcaatagct
     7081 tgtctaacac taggttttat ttcttcttgc aaggttttaa tggttaagta tgctccgacc
     7141 actaatacca gtgaaaatac taaccaaatc cgtaaaaaaa tagacattaa tattttgcca
     7201 aactataacc aatcccacga tgagttttaa tcacttcatc gtcatgaata tttttcagtt
     7261 tttgacgtaa cactttaata tgtgtatcga tagtacgctc taaactatga tcgggatgct
     7321 cccatgccgc ttgcattaat tgatgacgac tcaaaactcg ctcgggttgt tttaataaaa
     7381 cttctaataa acgatattca taacgtgtta aatttagtaa ttgaccatga tagctaatct
     7441 gcaatttttc gggatcatgt tgccaaattt tagcaagact attgctatgt ataggtgttg
     7501 cttgaggttg cagacgacga caaattaccc taatacgaga tactaattca cgcgggctaa
     7561 atggttttgc acaataatca tcagcaccaa tttctaaacc gataattcgt tccacttcat
     7621 cattacgtgc cgttaaaaat aataaggggg tttgatattg ttgacgaatt tgacgacaca
     7681 aatcaaaacc attaatgtca ggtaagccca catctaaaat aataaaatca acggggtgtt
     7741 gtgccacata gtctaatgct tgttgtccta aagcacacca ctgaacttgc catccttcac
     7801 gttgtaaact aaaaatcaaa gtttgggcaa ttgcttcatc atcttcaact aataaaatat
     7861 gctttaacac agcaagaatt tccctaaaaa aaacggtatt tgccatgaca gcaaataccg
     7921 tttttatatg ttatataatt ctaaaaatta cagcaactta cggcctttac ctgcggcaat
     7981 acgtaaacgt aaaccgttta agcgaataaa gccttcggcg tctttttgat tgtatgcacc
     8041 gcggtcatct tcaaaggtag caatattggc atcaaaaatc gaatcatccg atttacgacc
     8101 cgcaatataa acattacctt tgtacaactt aacacgtaca acaccgttta cataacgctg
     8161 agattcgtca atcatggttt gcaacatttg acgctcagga ctccaccagt aaccgttata
     8221 aaccaagctg gcataacgtg gcattaactc atcttttaaa tgcgcaactt cacgatctaa
     8281 ggtaatcgat tcaatcgcac ggtgagcctt catcataatt gtgccaccag gtgtttcata
     8341 acaaccacgt gacttcatgc ccacataacg attttcaact aaatctaaac gaccaatacc
     8401 gtgtttgcca cctaaagcat ttaattgcgt taatacttgg gcaggtgtca tttctgtgcc
     8461 gttaatcgcc acaatatcgc caccacgata ggttaattca acatattctg gttgatctgg
     8521 agctttttct gggctaactg tccaacgcca aatgtcttct tctggttccc aaaatgggtc
     8581 ttccaagcca ccaccttcat aagaaatatg caataagttt gcatccatag aataaggtga
     8641 ttttttgcct gctttattaa aatcaatagg aatattatgt ttggcggcat aattcattaa
     8701 tgtttcacgg ctggttaaat cccattcacg ccacggagca atcacgcgaa tattgggtgt
     8761 taaggcataa gcacctaatt caaaacgaac ttggtcatta cctttacctg ttgcaccgtg
     8821 agaaatcgca tctgcacctt gctcctgagc aatttcaact aaacgcttag caattaatgg
     8881 acgtgcaatc gacgtaccta ataaatactc gccttcataa atgg
//
LOCUS       DAOQMB010000050         8662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3220_length_8662_cov_14.271639, whole genome shotgun sequence.
ACCESSION   DAOQMB010000050 DAOQMB010000000
VERSION     DAOQMB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3220_length_8662_cov_14.271639"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PLY05_03210"
     CDS             <1..948
                     /locus_tag="PLY05_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02342.1"
                     /translation="LGNHIRQFTLDHALPVILKTDDVPMICNANDGLAPLVMAYGRFN
                     VEIDLMMGFGFAGSSICTENKAVEKELWSALAERQGWTDVAIDARISQQLLNKEAALK
                     QLKAYQHSVNYFKNEYGYELGEGKCPKFKYEVDELLLFVGAVSALQALQNDVASGRLV
                     NVDMGIPPKIVHAMECLDNQKWWGFPQSLQAALIVVLPESEEKQAEGWRKLQEAVDIG
                     LKDGMRLGHATYAVVAYIKGREDYLRDALKRFEAVPSDKINPDYKLLDAIAEKQIRHL
                     ANRLWMQKEGRRAPTEGYSDFLDEKRQPDPKLDGLLDDI"
     gene            complement(968..1579)
                     /locus_tag="PLY05_03215"
     CDS             complement(968..1579)
                     /locus_tag="PLY05_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02343.1"
                     /translation="MTQRAIAFRAREQAILDATEHLLLELGDEAITIEMIAERVGIAK
                     GTIYKHFQSKDELILHVLIAYEQELAMTMNDAVKTDDIHQVAQAYFSFRLATPEKHLL
                     FERLENKLAATRRTLRPYFARLYFIRKSYLRQALPIVARYLKEQNSSMTPRDYLTAGW
                     ALVQGTAAILHSSFYQRYLGDRQGLLAAMLETLQSLGERTYAH"
     gene            complement(1576..2352)
                     /locus_tag="PLY05_03220"
     CDS             complement(1576..2352)
                     /locus_tag="PLY05_03220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC02344.1"
                     /translation="MFIDSHCHLDRLDLQAYQGNLALALQAAREQGVSHFLTVSVELD
                     DYPVLSAFTEQYSDVSMSVGVHPSDVAAANQQPTVELLCQLAKHPKVVAIGETGLDYH
                     YHPEQAAVQQQSFITHILASKQCAKPLIVHTREARKDTIDLLKAHQAEQGVLHCFTED
                     WDTAKAALDLGFYISFSGIVSFANAKELREVAAKVPLDRILIETDSPYLAPVPYRGKK
                     NEPRYLPAVAKAVAEVRGLHFEEIAQITSANFGRLFGINI"
     gene            complement(2365..2709)
                     /locus_tag="PLY05_03225"
     CDS             complement(2365..2709)
                     /locus_tag="PLY05_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HNC02345.1"
                     /translation="MGLPRGAGIQTLQFKDKAQLYASYMPFMKGGALFFPTMKIPKLG
                     EELFAVIILPDETERMPLTSKVVWVNHRTHGNRLAGFALALAGIEGDNIRRKIEAQLG
                     GSLQSDKPTFTL"
     gene            complement(2741..3727)
                     /gene="holB"
                     /locus_tag="PLY05_03230"
     CDS             complement(2741..3727)
                     /gene="holB"
                     /locus_tag="PLY05_03230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HNC02346.1"
                     /translation="MNTVYPWQQAIWQILVQSRARQTLPHAFIFSGVQGVGLFEFSQV
                     MSAWLLCQQPQSDGACGVCKSCQLWQAQSHPDYRLVAQLVDDKGKTSQVIKVDQIREL
                     LDFLNKSAQLNGYRVAVIHHADTLNTNAANSLLKNLEEAGKNTAIILLTEQPLSLLAT
                     IRSRCQQFNFVVPDLAQAKVWLGKQLKQAQQADLLLALSAGAPLAALALQDQSWLEQR
                     HALAKAILAVLQQKQTALLALQSFHKQLEPEQQLKMLQLLFSDVLFSKLGQQQAIKNS
                     DLLPIINSMAECLSAQQIIALQQQCLEGLRLIAANIQANLILENLWFSLRVA"
     gene            complement(3724..4353)
                     /gene="tmk"
                     /locus_tag="PLY05_03235"
     CDS             complement(3724..4353)
                     /gene="tmk"
                     /locus_tag="PLY05_03235"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNC02347.1"
                     /translation="MPVFISLEGTEGVGKSTVLNFIQAYLQEHHVPFVCTREPGGTPL
                     AEKIRHLLLSKDDEKMAYHTELLLMFAARAQHISQVIKPALQHKKWVISDRFTDASYA
                     YQGGGRGLPVAEIALLENMVQSDLQPNITFWLDAPVEIGLARAAARQQLDRFEQEKVD
                     FFQRVRAVYAQRAETYPQRYRRIDASLSVEQVQAQVKQVLAHLVAIHTL"
     gene            complement(4355..5539)
                     /gene="mltG"
                     /locus_tag="PLY05_03240"
     CDS             complement(4355..5539)
                     /gene="mltG"
                     /locus_tag="PLY05_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNC02348.1"
                     /translation="MSEQVPKTPRNSKTKSSETQTKARSNRSKSRTKPTQQAEKKPRV
                     TTKKTISQKNNTVKKNYLKVVFASLIVVILAVSLWLYQGLFSALAMPSTGYKISIEKG
                     SSYSQVLQQLEKDQLLPSALLAKAYLKVNAPKNLQIGTYLFKQPISLVRLLTQLSKGE
                     GLVMTKITVIEGTTFKQLRQQIEKNSEIKQTLLGKSDVEVLAMLGVPIQHPEGLFAPD
                     TYIFALDDTDLKVLKRLYQQQEKILNKAWQNRAENLPYKTPYEALIMASIVEKETGAA
                     AERPLIAGVFINRLRIGMRLQTDPTVIYGMGDNYNGNIRKSDLLTYTPYNTYKINGLP
                     PTPIALPSKAAIEAALQPAKTQALYFVAKGDGSGEHTFSDSLAAHNQAVADYLQTRRE
                     RR"
     gene            complement(5532..6341)
                     /gene="pabC"
                     /locus_tag="PLY05_03245"
     CDS             complement(5532..6341)
                     /gene="pabC"
                     /locus_tag="PLY05_03245"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:NF004761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HNC02349.1"
                     /translation="MLACWLNGQAIQTLSLTERAFNYGDGLFSTIAIRQGQASLIDLH
                     WQRLVQGCRRLLIDCQNLAQWQEDFLRFMALYPNCIAKIIVSRGVGGRGYLPEANQQT
                     HCYFYAYPSHHHPQHYQQGIDTDFLQQQLSCGKLLAGLKHLNRLEQVLLRQELAQTSF
                     PEAIVCNEQGYVIEGVFSNLFIIKNGQLQTPRLDLSGVDGVMRRHLLSLAQQILLPTQ
                     IINISPSDMLTADEMFFCNSLYGIWPVKRLAEKTFAQNPVTQQLQSLLHYV"
     gene            6636..6971
                     /locus_tag="PLY05_03250"
     CDS             6636..6971
                     /locus_tag="PLY05_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02350.1"
                     /translation="MINKLDKTASLVPSVDLPIDASTEHLELRLLPYWSGVKHFRNNA
                     LFNHHTPQDYYWQILRHNQNPTVVDGKIVGFHTRSDMYLSRLPHCLQHKIWQNFGIAS
                     NDSFHSSRC"
     gene            7073..8482
                     /locus_tag="PLY05_03255"
     CDS             7073..8482
                     /locus_tag="PLY05_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC02351.1"
                     /translation="MSQSFQLKEQFSVARPIEQVFDYLANFSQIADWDPSVFSAKKIS
                     QGAIKQGSQFDLIIKSAGRKLPMAYELIDYQPYHSLTFLGKAEGFTATDRIVLKAQND
                     HTHITWQADLEFTGVLQYLIPVMRRSLQQLGQNSMRGLEQALNDNYPVPIAASESLKQ
                     KLVLPALWPFTKYGYLQAQKNWHPVSASIKNKHVVITGTSSGLGLAAAKSLAAKGADL
                     TLVMRDANKAKSIQASLIKETGNPHIHIELADLSLMNDVLALSQRLIKNAKPIDVLIN
                     NAGALFNPRQQTKEGLEQSFALLLLSPFILTEQLKPLLIKGSRVINVLSGGMYSQKID
                     VDDLENKGGAYSGSVAYAKAKRGLMITTEQWANTWQAAGICVHAMHPGWAETTGVISA
                     LPEFYRLTKPLLRSPEQGADTIVWLATATEAGKCTGLFWLDRQPQATHLLAKTQETAQ
                     QREQLRLSLLSYQQQFLHR"
     gene            complement(8523..>8662)
                     /locus_tag="PLY05_03260"
     CDS             complement(8523..>8662)
                     /locus_tag="PLY05_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003664010.1"
                     /note="FabF; beta-ketoacyl-ACP synthase II, KASII;
                     catalyzes a condensation reaction in fatty acid
                     biosynthesis: addition of an acyl acceptor of two carbons
                     from malonyl-ACP; required for the elongation of
                     short-chain unsaturated acyl-ACP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HNC02352.1"
                     /translation="NLDNPDPACRLDYVPHQARPMKIDVALSNSFGFGGTNGSLIFKR
                     V"
BASE COUNT         2636 a         1743 c         1579 g         2704 t
ORIGIN      
        1 ttgggcaatc atattcgtca attcacttta gaccatgcct taccagttat cttaaaaact
       61 gatgatgtgc caatgatttg taatgccaat gatggtttag cacctttagt catggcctat
      121 ggcagattta atgttgaaat tgatttaatg atggggtttg gttttgcagg ttcttctatt
      181 tgcaccgaaa ataaagccgt tgaaaaagaa ttatggtctg ctttagccga aagacaaggt
      241 tggactgatg ttgcgattga tgcgcgtatt agtcagcaac ttctcaataa agaagctgca
      301 ctaaaacagc ttaaagccta ccaacacagt gtaaattatt tcaagaacga atatggctat
      361 gagctaggcg aaggtaaatg ccctaagttt aaatatgaag ttgatgagct attgctcttt
      421 gttggtgctg tcagtgcttt acaagcctta caaaatgacg ttgcttcagg cagattagtt
      481 aatgtggaca tggggattcc tcccaaaatt gttcatgcca tggagtgttt agacaatcaa
      541 aaatggtggg gatttccaca atccttacaa gcagctttaa ttgttgtttt acctgaaagt
      601 gaagaaaaac aagctgaagg ttggcgaaaa ttacaagaag cggtagatat tggcttaaaa
      661 gatgggatgc gtctaggtca tgccacctat gctgttgttg cgtatattaa aggccgtgaa
      721 gattatttgc gtgatgcgct aaaacgcttt gaggctgttc ctagcgacaa aatcaatcca
      781 gactataagt tacttgatgc gattgctgaa aaacaaattc ggcatttagc taatcgcctg
      841 tggatgcaaa aagaaggccg ccgcgcacca actgagggat atagtgactt tttagatgaa
      901 aaaagacaac cagaccctaa actggatggt ttattagacg atatttaggc taaatttgga
      961 cttatgtcta gtgtgcataa gtcctttccc ccaaactttg caaagtctct aacatcgctg
     1021 ccaacagtcc ttgtctatcg ccaagataac gttgataaaa actggaatgc aaaattgcag
     1081 ctgttccctg aactaacgcc caacccgctg ttaaataatc acgtggggtc atgctactat
     1141 tctgctcttt taaatagcga gcaacaatag gcaatgcttg tcgtaaataa cttttacgaa
     1201 taaagtataa acgtgcgaaa taaggtctta atgttcgtct tgttgctgct aatttatttt
     1261 ctaaacgttc aaataataaa tgcttttcgg gggttgccaa tctaaaacta aaataagctt
     1321 gggcaacttg atgaatatcg tctgttttta ccgcatcatt cattgtcatt gctaactctt
     1381 gttcgtaagc aatcaacaca tgtaaaatta attcatcttt actttgaaaa tgtttataaa
     1441 ttgtcccttt cgcaatacca actcgttcag caatcatttc tatcgtaata gcttcgtcac
     1501 caagctccaa taataaatgc tctgtcgcat ctaaaattgc ttgctcacgg gctctaaaag
     1561 cgattgcacg ttgagtcata tatttattcc aaataaacgt ccaaaatttg cgctggttat
     1621 ttgtgctatt tcttcaaaat gaagccctcg cacttccgca actgctttag ctactgctgg
     1681 caaataacga ggttcatttt ttttacctcg atacgggaca ggtgctaaat acggcgaatc
     1741 tgtttcaatt aatattctgt ctaaaggtac ttttgctgca acttctcgca attctttggc
     1801 attagcaaag ctgacaatgc ccgaaaaaga aatgtaaaag cctaaatcta aagctgcttt
     1861 tgccgtatcc caatcttcgg taaaacaatg taagacacct tgttctgctt gatgcgcttt
     1921 taacaaatca atcgtatctt ttcttgcttc gcgagtatgg acaattaagg gtttggcgca
     1981 ttgtttactc gccaaaatat gagtgataaa gctttgctgc tgaacagccg cttgctcagg
     2041 atgataatga taatctaaac ccgtttcgcc aatagccacg actttaggat gttttgctaa
     2101 ttgacacaac aactcaaccg ttggctgttg attcgctgct gccacatcgg aaggatgcac
     2161 accaacggac atggatacgt cactatattg ctcagtaaac gcggataaaa caggatagtc
     2221 gtctagttct acactcactg ttaaaaaatg actcacgccc tgttctcttg ctgcttgtaa
     2281 agcaagagct aaatttcctt gataagcttg taaatctaat cggtctaaat ggcaatgcga
     2341 atctataaac attgatttat ttctttacaa agtaaatgtt ggtttgtctg attgcaacga
     2401 accacctaat tgggcttcaa ttttacggcg aatattatca ccttcaatgc ctgctaaagc
     2461 taaggcaaaa cctgccaaac gattaccatg agtacgatga ttaacccata ccacttttga
     2521 tgttaacggc attctttcgg tttcatcagg caaaataatt accgcaaata actcttcgcc
     2581 taattttgga attttcatgg ttggaaaaaa caatgcgcca cctttcataa acggcatata
     2641 actggcatat agttgtgctt tatctttaaa ttgcagggtt tgaatacctg caccacgcgg
     2701 taatcccatt ttattcacct attatcaaat agtgtagtgt tcaggcgaca cgtaatgaaa
     2761 accaaagatt ttctaaaatt aaattagctt gaatatttgc tgcaattaaa cgcaaacctt
     2821 ctaagcattg ttgttgtaat gcaataattt gctgcgctga taagcactca gccatgctgt
     2881 tgattatagg caataaatca ctatttttaa tagcctgttg ttggcctaat ttgctaaata
     2941 acacatctga aaacagcaat tgtagcattt ttaattgttg ctctggttct aattgcttat
     3001 gaaacgattg taaggccagc aatgccgttt gcttttgctg taatactgcc aaaattgctt
     3061 tggctaatgc gtggcgttgc tcaagccatg attggtcttg taaagctaat gccgctaatg
     3121 gcgcacctgc actaagggca agcagcaaat ctgcctgttg tgcttgtttt aattgtttgc
     3181 ctaaccatac tttagcttga gcaagatcag gcactacaaa attaaattgt tggcaacgac
     3241 tgcgaatagt tgctaataaa ctcagtggct gttctgttaa taagataatt gcggtatttt
     3301 ttcctgcctc ttccaagttt tttaataaac tgttggcggc attggtattt aaagtatcag
     3361 catgatgaat caccgccacc cgataaccat taagttgtgc tgatttgttc aaaaaatcta
     3421 acaattcacg aatttgatca actttaatca cttggctagt tttgccttta tcatcgacta
     3481 attgagcgac caaacgataa tctggatgac tttgtgcctg ccataattga caacttttac
     3541 acacaccaca cgcgccatct gactgaggtt gttgacacaa tagccacgcg ctcatcactt
     3601 ggctaaattc aaacaaacca acaccttgca caccactaaa aataaaggca tgaggcaaag
     3661 tttgacgcgc acggctttgc actaaaattt gccaaatagc ctgctgccaa gggtaaactg
     3721 tgttcataaa gtatgaatcg caactaaatg tgccaagact tgttttactt gtgcttgaac
     3781 ttgttcaaca ctcaaactag catcaatacg tcgataacgt tgcggatagg tttcggcacg
     3841 ttgggcatat acggcgcgaa cacgttgaaa aaaatcaact ttttcttgct caaaacgatc
     3901 tagctgttgg cgggctgcgg ctcttgccaa acctatttct acaggcgcat ctagccaaaa
     3961 ggtaatattg ggttgcaagt cactctgtac catattttct aataaggcta tttcggcaac
     4021 aggtaatccg cgcccaccac cttgataagc ataacttgca tcggtaaatc tgtcagaaat
     4081 cacccacttt ttatgttgta atgctggttt aatcacctga gaaatgtgtt gtgcgcgcgc
     4141 tgcaaacatc aacaataact cggtatgata agccattttt tcatcatctt tactcaataa
     4201 taaatgacga attttttcgg ctaaaggtgt gccaccaggt tcacgcgtac agacaaaagg
     4261 cacatgatgc tcttgcaaat aagcctgaat aaaatttaaa acggtgcttt taccaacccc
     4321 ttccgtacct tctaaactaa taaaaacagg catgttatct tctctctcga cgggtttgta
     4381 aatagtcagc aacggcttga ttatgtgcag ctaaggagtc actaaaggta tgctcaccac
     4441 tgccatcacc cttggccaca aaatataaag cttgagtttt tgcaggctgt aacgcggcct
     4501 caatcgctgc tttactaggc aaagcaatag gagttggcgg taatccattg attttataag
     4561 tattatacgg cgtataagtt aataaatcac ttttacgaat attaccatta taattgtcac
     4621 ccatgccata aatcacggtt ggatcagttt gtaagcgcat tcctatccgt aaacgattaa
     4681 taaatacacc agcaatcaat ggccgttccg ctgctgcacc cgtttctttt tcaacaatag
     4741 acgccataat taaggcttcg taaggtgttt tatacggcaa attttcggct ctattttgcc
     4801 aagccttgtt taagattttt tcttgttgtt gatataaacg ttttaacact tttaaatctg
     4861 tatcatcaag cgcgaaaata taagtatcag gtgcaaataa accttctgga tgttgtattg
     4921 gtactcccaa cattgctaat acttcaacat cacttttgcc aagtaaggtt tgtttgatct
     4981 ctgaattttt ttcaatttgc tgacgaagct gcttaaacgt tgttccttca atgacggtga
     5041 ttttggtcat taccaatcct tcacctttac tcagttgtgt taacaaacgt actaaagaaa
     5101 tcggttgctt aaataaatat gtacctattt gtaagttttt aggggcatta acttttaaat
     5161 aggctttagc caataaagca cttggcagca actgatcttt ttctaattgt tgaagcactt
     5221 ggctataact actgcctttt tctattgaaa tcttataacc cgtactcggc attgctaaag
     5281 cactaaataa tccctgatac aaccataaac taacagccaa tatcaccact attaacgatg
     5341 caaaaacgac tttaaggtaa ttttttttaa cggtgttatt tttttgcgaa atcgtttttt
     5401 tagtcgtcac tcgtggcttt ttctctgctt gctgagttgg tttagtacga cttttactgc
     5461 gattgcttcg agctttagtc tgagtttcac tagacttagt tttagaattg cgtggtgttt
     5521 tgggtacttg ttcagacata atgtaataaa ctttgtaatt gttgcgtcac aggattctgt
     5581 gcaaaggttt tttcagcaag ccgtttaaca ggccaaatac catataaact attgcaaaaa
     5641 aacatctcat cagccgttaa catatcgctt ggtgatatgt tgatgatttg tgttggcaat
     5701 aaaatttgtt gcgccaaaga taacaaatga cggcgcataa ctccatctac acctgataga
     5761 tctaaacgag gtgtttgtaa ctgaccattt tttataataa ataaattact aaaaacgcct
     5821 tcaatgacat agccttgctc attacaaaca atcgcttcag gaaaagatgt ttgtgccaat
     5881 tcttgacgta acaaaacttg ctctaaacga ttaagatgct ttaaacctgc taacaacttt
     5941 ccacaagaca attgctgctg caaaaaatcg gtatcaatac cttgttgata atgttgtgga
     6001 tgatgatgac tcggataagc ataaaaataa caatgggtct gctgattggc ttcgggtaaa
     6061 taacctctgc cccccacacc acgactgaca ataatcttag ctatacaatt cggatacaat
     6121 gccataaacc gcaaaaaatc ttcttgccac tgagccaaat tttgacaatc aatcaataat
     6181 cgtcggcaac cttgcactaa acgttgccaa tgaaggtcta ttaaactcgc ttgtccttga
     6241 cgaatggcaa ttgttgaaaa caagccatca ccataattaa aggctctctc agtcagagat
     6301 aaggtttgta tagcttggcc atttaaccaa cacgctaaca tattgttctc caaaaccttt
     6361 tcattatcat gctcagttaa tgtcatattt tgttgcaaag ttgcgatgca gcacacagct
     6421 tttaagtcta ttctttgaac agtcaacttt tatctatcaa tcaatgttta actgatatac
     6481 agttgcaatt ttaataaggt attactttat attttttaga taatttgttg atttttaaat
     6541 aaattaaaaa ttttacaaaa gccatcattt agggtatttt agcacatctg cttgtggcca
     6601 aaaacaggga tagtaactct agagggtatt taataatgat taacaagctc gacaaaactg
     6661 cgtctttagt tccttcagtt gatttaccaa ttgacgcatc gacagaacat ttagaattac
     6721 gcctattgcc ttattggtct ggtgttaaac actttcgtaa taacgcccta tttaatcacc
     6781 atacgcccca agactattac tggcaaattt tacggcacaa ccaaaacccg accgttgttg
     6841 atggtaagat agtcggattt catactcgct ctgatatgta tttatcccgt ctcccccact
     6901 gtttgcaaca taaaatctgg caaaattttg gtatcgcaag taatgatagt tttcacagct
     6961 ctcgctgtta gggcttaggt tctgttgatt agcataaaaa taactttgtt ctattgcttt
     7021 aaataatcac ggcttttatt ttagctcgat aatttttatt gagcctacaa caatgagcca
     7081 atcttttcag cttaaagaac aattttcggt agcacgtcct atcgagcaag tctttgacta
     7141 cttggcaaat tttagccaaa ttgccgattg ggatccgagt gtctttagtg ccaaaaaaat
     7201 atctcaaggt gccattaaac aaggtagtca atttgattta attataaaat cagcaggccg
     7261 aaaactacct atggcttatg agttaattga ttatcaacct tatcactcat taactttttt
     7321 aggtaaagca gaaggcttca ccgccaccga ccgcattgtt ttaaaagcgc aaaatgacca
     7381 tacgcatatt acttggcaag ctgatttaga atttacaggt gttttacaat atcttattcc
     7441 cgtgatgcgt cgctcattgc agcaattagg ccaaaactcc atgcgtggcc tagaacaagc
     7501 tcttaatgat aattatcctg ttcctatagc tgcatctgag tccttaaagc aaaaattagt
     7561 tttacctgct ctatggcctt ttacgaaata tggctatttg caagcacaaa aaaattggca
     7621 tcctgtttct gcgtcaatta aaaacaaaca tgttgtgatt actggcacta gctctggctt
     7681 aggcttagca gccgctaaat cattagctgc taaaggggct gatcttaccc ttgttatgcg
     7741 tgatgcaaac aaagcaaaat ctattcaagc gtctttaatc aaagaaacgg gaaatccaca
     7801 cattcacatc gaattggccg atttaagcct aatgaatgat gtgttagcac taagtcaacg
     7861 attgataaaa aatgctaagc cgattgatgt cttaattaat aatgctgggg cattatttaa
     7921 tccacgccaa caaactaaag aaggtttaga gcaaagcttt gctttattac tattatcgcc
     7981 ttttatatta accgaacaac taaaaccttt attaatcaaa ggctctagag tgattaatgt
     8041 tttgtcaggc ggtatgtata gccaaaaaat tgatgttgat gatctagaaa ataaaggcgg
     8101 agcttattct ggcagtgttg cttatgctaa ggctaaacgt ggcttaatga taacaaccga
     8161 acaatgggca aacacgtggc aagcggcagg gatttgtgtt catgctatgc atcctggttg
     8221 ggctgaaaca acaggtgtta tcagtgcgtt acctgaattt tatcgtttaa ctaaaccttt
     8281 attaagatca ccagagcaag gtgctgatac cattgtttgg ttggcaaccg ctacggaagc
     8341 aggcaaatgt acaggacttt tttggttaga tagacagcct caagccacac atttattagc
     8401 taaaacccaa gagaccgctc aacagcgtga gcaattacgc ttgtctttat taagctatca
     8461 acaacagttt ttacatagat agaagtatgt tgggtatctc atacccaaca tacctaaagc
     8521 cactaaaccc gtttaaaaat caatgaacca ttagttccac caaaaccaaa agaatttgac
     8581 aaagcaacat caatcttcat cggacgcgct tgatgcggca cataatctaa acgacaagca
     8641 gggtcagggt tatctaaatt aa
//
LOCUS       DAOQMB010000051         8645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3230_length_8645_cov_16.125844, whole genome shotgun sequence.
ACCESSION   DAOQMB010000051 DAOQMB010000000
VERSION     DAOQMB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_8645_cov_16.125844"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..1559
                     /locus_tag="PLY05_03265"
     CDS             336..1559
                     /locus_tag="PLY05_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNC02353.1"
                     /translation="MRSWGKTPHVVVALNCLCFSMAYAEEAATATSVPAATSADTSDV
                     KPSVESASVQANPIDNKAPEAKSEATAEAKPTPADSKAETKTEAADKAREALKQKSTD
                     TNAEKDLEKVFQSAEKTYSLSKKGKYAMNYDLGYSYYRDKQIDIALEPNGSTIQRLRI
                     QDDSQHSFTNTVDVSYGLKDNITLSLSLPLIYKMDTAGGKEAVGLGDVALGMRWQPIP
                     LKRGLPTTTLYSSLSLATGDSPYEINSSKDLSTGKGYYSLSAGVSVSKVIDPIVMYGS
                     VGLTTGNSMGGLNQAQSGRTLTSFSPGQTLSTSVGLAYSLNYDVSISASYQQSISTNA
                     RYTFLQSNDEGKPETVSFNSATQLSSLLNYTLSLRTSPTRIVNFSVGFGLSEDTPDVN
                     LGLSMPLEFLGLASE"
     assembly_gap    1613..1712
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1713..3116
                     /locus_tag="PLY05_03270"
     CDS             <1713..3116
                     /locus_tag="PLY05_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNC02354.1"
                     /translation="PKDFEVFGYSDDPVVCNDPVNDEPLCSEFKTAHTKVDGVALYLP
                     VLDQIVKLPPGPVTAPTAGIAYKPPGSKFTYANAFYAPMIAGYYRDQDDPGVYMGRKV
                     ALERITYLSPSVAYKINDELSVGLSIGMSYQAIALDTDFRAPNPLVGVFRLFDEEICG
                     PFREEANLVTDLLLFGICNAQESLGPFKKAANLDVSLQQTLSPTYNLGVLWEPNEKFA
                     WGMVYQSADKMRLKGKYKVTYANGARSLINDGLQSSVTGQILGAILGLPSYIPESEEG
                     LLSMNLTFPAHFQTGIKYKILPQLQVNFDVGWTDFNRWDAFNFEFDREVQVLKIAKIL
                     VPGTTNTTLPFPLEFNSRWNWGIGLEYAHTSRLKFRMGYEPRDSAVPLNKRNILAPIN
                     SAKMFGLGLGYRFDRDTDIDLTLMHMYSRDTVPAGTSTLLNQTGVNNAMYNPYAGLDA
                     KTKAVINIMGLVYRTRW"
     gene            3116..3973
                     /gene="filE"
                     /locus_tag="PLY05_03275"
     CDS             3116..3973
                     /gene="filE"
                     /locus_tag="PLY05_03275"
                     /inference="COORDINATES: protein motif:HMM:NF033645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative pilus assembly protein FilE"
                     /protein_id="HNC02355.1"
                     /translation="MNNLVRLFSLVLLCASAVQAQERIYSTVDAQGRVQVIKSEAIDN
                     TKQETDVNTPVKPVIQTPSKYELEGEQYIDSDTLIKQQSEQPAKKRFYYVPTGALGEK
                     VLESEHDVAVSTLNKKTQLPQQEIKFSPEYQIITKEALLSKWHNLSSYCQQTKRLKPS
                     RLFKASNALWIDKSDFSANQPDKILSLEKKLLTAEVVRISSFANSQKKPKFYLPIIVF
                     LDEQGCVLEGAWQYWSYAKAANDNQYSAVDGLLTLPTSSKYILLYPPDEQVKISLPLQ
                     NYGSLLIEK"
     gene            4045..5919
                     /locus_tag="PLY05_03280"
     CDS             4045..5919
                     /locus_tag="PLY05_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02356.1"
                     /translation="MKLSSRLLLLPAMISLLMACGEESNTLSNPNAKNNDAETTTTCQ
                     DKDYCIATQFLDEPVVGLNYQCNSIEGVTDEDGVFLCPNNSVASFFLKSASGNKKIEL
                     GKYRVKTLGSPSDGSPVKLNLVATPEDLVAQIDGAVIDNKQLTNILRLLQALDSDGSS
                     PNRVINRIVLDSKDKFAIDELVSSVPLTVFAADDFDKVLKPMFDKLAPKSLANVGAAQ
                     AQARFNDSLIAINAGIYEVVPTFIKESDNVYNGMFARWTGSNLYSMVSMFFLVDRDGK
                     TIGTALEWQKELSTDDLNNSLVFKDFVVKTVPQDLFFNTNEPLFTTKGNVIPANFVLK
                     TALGDSIKVTRGVLSKNNISSGEAAYRFAYGLTQAQAVDVSQLGAWQRESATGISQIS
                     TGTLSLQKTRTPNMYLDGNFWRTKDNIVTGEKPIFPLHLKLTLKDSNTSTACSSTGCL
                     IGDIGITILENGNIISDRNNDCSVVDSSTLKDTLDMQEQRLGFVASVLVDRTQKATSE
                     SLIAPVMLIGDWAKKLPSSDVWYKLYGFHLGAISGMSGGPKVQIDVARATTGIVTIKN
                     QQDEREKLGEVALWANYVNYLKYLSESNTEKKKELGYASLGAVTNIQTQACYNPQPKI
                     "
     assembly_gap    5952..6051
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6052..6533)
                     /locus_tag="PLY05_03285"
     CDS             complement(<6052..6533)
                     /locus_tag="PLY05_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HNC02357.1"
                     /translation="MPNIIKDGAIVADTYQLVTEAGALPAQDIVVSLDVWQQQREAIL
                     AHPYKKAVLLKPDQHPEVLVEDVKQLDMIALDFPAFADGRGYSYATLLRQRFGFTGEL
                     RATGDVFKDNLFYLKRCGFNSFAVRADKDIHVALQGLNNFSESYQASVDESRPLYRRR
                     "
     gene            complement(6526..8178)
                     /locus_tag="PLY05_03290"
     CDS             complement(6526..8178)
                     /locus_tag="PLY05_03290"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HNC02358.1"
                     /translation="MYQYDAYDQKIVDQRVAQFRDQTQRYLNQELTEDQFRPFRLQNG
                     LYVQRYAPMLRVAIPYGMLSSVQLRTLANIARDYDRGYAHISTRQNIQYNWPELARVP
                     DILADLAKVQMHAIQTSGNCIRNTTTDQFAGVVAGEIIDPRPTCELIRQWSTFHPEFA
                     FLPRKFKIAVNALPDTDRAATAVHDIGIYIVKNEAGEIGYKVLVGGGLGRTPIIGSII
                     REFLPQNELIAYLDAVLRVYNLYGRRDNKYKARIKILVKALTPEVFAQKVEAEFAHTK
                     TEAMLVPAEEFARLASFFTEPAYQSLTDSPTALTDALSSNKAFALWYQHNTHAHKKAG
                     YRIVTLSLKRTGVAPGDITSEQMLTVADLADQYSFGELRTTHEQNIVLADVRQEQLFD
                     LWQQLSALDFATANIGFLTNIICCPGGDFCSLANAKSIPIAEQIQRRFEDIDQVYNIG
                     HLDINISGCMNACGHHHVGHIGILGVDKKGEEYYQVSLGGNSNHDTRLGDILGPSFYA
                     EEMADVIEEILNVYLDNRQENERFLDTYRRIGLAKFKERVYA"
     gene            complement(8325..>8645)
                     /locus_tag="PLY05_03295"
     CDS             complement(8325..>8645)
                     /locus_tag="PLY05_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004658508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC02359.1"
                     /translation="KCSTTYQLQKMMLSQPLGTLSEEDAAAALFISKRTLARHLAKEG
                     SSYMEIREKLLSEQASHYLRNSTMSVEAIASLLNYHDSANFRRAFKRWFAVSPKEYRQ
                     QFIV"
BASE COUNT         2642 a         1466 c         1759 g         2578 t
ORIGIN      
        1 acaattacgc agttagcttt tatggagatg gattataatt tgcggcaggc agagggagat
       61 tatcataaag cggcaacgat gcagcgtgtc tttaatagtg atacagaggc tactgatgat
      121 aaaaaaatta ttgatgtgcc attgcgtatg tattatcgcc gtaaataagc ttttgattga
      181 gtctgtttaa gttattattt ttgtaaaata aacagcaaat acctaatcat ttagttggta
      241 atgctaattt gtagcaaaat taaactaagt gctaatttaa ataaaaacga tttattaact
      301 ttgttaattg ataagaccta taaggaatca caacaatgag aagctggggt aaaacaccac
      361 acgttgtagt tgctttaaat tgcttatgtt tttcaatggc ttatgctgaa gaagctgcta
      421 ctgccacaag tgtgcctgct gcaacatcgg cagatacgag tgatgttaag ccttctgttg
      481 agtcggcatc agtacaagcc aatccaatag ataacaaagc accagaagct aaaagcgaag
      541 caacagcaga agcaaagcct actccagcag atagcaaagc ggaaactaaa actgaagcag
      601 cagacaaagc gcgtgaggca ctcaaacaaa agtccactga cactaatgct gaaaaagatt
      661 tagaaaaagt tttccaatca gcggaaaaaa cctattcatt gtctaaaaaa ggcaaatatg
      721 cgatgaacta tgatttggga tatagttatt atcgtgataa acaaatagat attgctttag
      781 agccaaatgg ttcaactatt cagcggttac gtattcaaga tgactcacag cattcattta
      841 cgaatacagt tgatgtgtct tatggtttaa aagacaatat tacattaagc ttatcattgc
      901 ctttaattta taagatggac acagcaggcg gaaaagaagc cgtaggatta ggcgatgttg
      961 ccttaggtat gcgttggcag ccgattccct taaaacgtgg tttacctaca acaactttat
     1021 atagctcttt gtctttagca acaggcgata gcccttacga aattaattca tcaaaagatt
     1081 tatcaacagg taaagggtat tatagtttgt cggcaggcgt gagtgttagc aaggttattg
     1141 acccaattgt gatgtatggt tctgtgggct taaccacggg taattcgatg gggggcttaa
     1201 atcaggctca aagcgggcgt acattaacga gcttctcgcc aggtcaaaca ttgagtactt
     1261 ctgttggttt ggcatattct ctaaattatg atgtgtcaat tagtgcgtcc taccagcaaa
     1321 gtatatcaac taatgctcgt tatacctttt tacaaagtaa tgatgaaggt aagccagaaa
     1381 ctgtcagttt taattctgcc acacagcttt cttcattgtt aaattacacg ctgtctttac
     1441 gtactagtcc aacacgcatt gtcaatttta gtgttggttt tggcttatca gaagatacgc
     1501 cagacgtaaa cttgggtctt tctatgcctt tagaattttt gggtttagct agcgaataat
     1561 ataagggtat agcaaatgat aggctctata ttaaaaagac ctaaattatc aannnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccaaaaga ctttgaagtt tttggttatt
     1741 ctgacgatcc tgttgtttgt aatgatccag ttaatgatga gcctttatgt tccgagttta
     1801 aaacagctca tacgaaagta gatggtgttg ctctatatct acctgtttta gatcaaatag
     1861 taaagctacc accaggtcct gtcactgcac caacagcagg gattgcctat aagccaccag
     1921 gctcaaaatt tacttatgct aatgcttttt atgcgccgat gattgcaggt tattatcgtg
     1981 atcaagatga tcctggtgtc tatatggggc gtaaggttgc attagagcgg attacttatt
     2041 tgtcgccatc tgttgcctat aaaatcaatg atgaattgtc tgttggtttg tcaattggta
     2101 tgtcatatca agcgattgca ttagatacag actttagagc cccgaaccct ttagtcgggg
     2161 tttttcgatt gtttgatgaa gaaatttgtg gtccatttag agaggaggct aacttagtaa
     2221 cagacttatt gttatttggt atttgtaatg cacaagagag cttagggccg tttaaaaagg
     2281 cggctaattt agatgtctct ttgcaacaaa cattatcacc aacctataac ttaggtgtgt
     2341 tatgggagcc aaatgagaag tttgcatggg gaatggtgta tcaaagtgcc gataaaatgc
     2401 gcttaaaagg caaatataaa gtgacttatg ccaatggtgc gcgttctttg attaatgatg
     2461 gtttgcaatc atcagttaca ggacaaattt tgggggctat tttaggcttg ccaagttata
     2521 taccagaatc ggaagaaggt ctgttgtcaa tgaatttgac ctttcctgct cactttcaaa
     2581 cagggataaa atataaaata ttacctcagt tgcaagtaaa ttttgatgtg ggttggacgg
     2641 attttaatag atgggatgcg tttaattttg agtttgaccg cgaagtacag gtattaaaaa
     2701 ttgctaaaat tttagtacca ggtacaacaa ataccacttt accttttcct ctggagttta
     2761 atagccgttg gaactggggt attggtttag aatatgctca tactagtcgt ttaaagttta
     2821 gaatgggtta tgaaccgcgt gatagtgctg tacctttaaa taaacgtaat attttagcac
     2881 caattaattc ggcaaaaatg tttggtttgg ggcttggcta tcgttttgat cgtgatactg
     2941 acattgattt aacattaatg cacatgtaca gtcgagacac tgtaccagca ggaacaagta
     3001 ccctattaaa ccaaaccggg gttaataatg cgatgtataa cccctatgct ggtctggatg
     3061 ctaaaactaa agcagtcatt aatattatgg gacttgttta tcggacccgt tggtaatgaa
     3121 taatttagtt cggttattca gtttggtttt gttgtgtgca tctgccgttc aagctcagga
     3181 gcgtatttac agcactgtag atgcgcaagg gcgtgttcag gttattaaga gtgaagctat
     3241 agacaataca aagcaagaaa ccgatgttaa tacacctgta aagccagtta ttcaaacacc
     3301 tagtaaatat gagcttgagg gtgagcaata tattgattcc gatacattga ttaagcaaca
     3361 gtccgagcaa cctgccaaaa aacgttttta ttatgttcct acaggtgcat taggtgaaaa
     3421 agttttagaa agtgagcatg acgttgctgt ttcaacgctt aataaaaaga ctcaattacc
     3481 ccagcaagaa atcaaatttt cgcccgaata tcaaatcatt acaaaagaag cattgttatc
     3541 taaatggcat aatttatcat cgtattgcca acaaactaaa cgattaaagc ctagtcgtct
     3601 gtttaaagca tccaatgcct tatggattga caagtcagat tttagtgcta atcagccaga
     3661 taaaatcctg agcctagaaa aaaaattatt aacagctgag gttgtacgta tctctagttt
     3721 tgccaatagt caaaaaaagc caaaatttta cttgcccatc attgtttttt tagatgaaca
     3781 agggtgtgta ttagaggggg cttggcaata ttggagttat gccaaagcgg ctaatgataa
     3841 tcaatatagt gcggttgatg ggttgttaac attgccaaca tctagcaaat acatactttt
     3901 gtatccgcct gatgaacaag taaaaataag tttaccatta caaaattacg gctcattgct
     3961 tatcgaaaaa taatttaact ggcttaatca acgcgtaagt catataactt taaagtgcgt
     4021 gccatattaa tattggagaa ctgtatgaaa ttatcgtctc gtttgctgct gttgccagcg
     4081 atgatttcgt tattaatggc ttgtggtgaa gaaagtaata ctttatcaaa tcctaatgct
     4141 aaaaataatg atgcagaaac aacaacgacc tgccaagata aagattattg tattgctact
     4201 cagtttttag atgagcctgt tgttggttta aattatcaat gtaattcgat agaaggcgtg
     4261 actgatgaag atggggtttt tttatgccct aataattcag ttgcatcgtt ctttttaaag
     4321 tcagcaagtg gtaataaaaa aatcgaattg ggtaaatatc gcgttaagac attaggaagt
     4381 ccatctgatg gtagtcccgt caaattaaac ttagtggcaa ctccagaaga tcttgttgct
     4441 caaattgatg gtgcggtgat tgataataaa caattaacga atattttacg cttgttgcag
     4501 gctttagaca gtgatggcag cagcccgaat cgtgtgatta atcgtatcgt attggatagt
     4561 aaagataaat ttgcgataga tgagcttgtt tcaagtgttc ctttaacagt atttgctgct
     4621 gatgattttg ataaagtttt aaagccgatg tttgataagc tggcaccaaa atctttagcc
     4681 aatgtgggtg cagcgcaagc acaagcgcgt tttaatgact cattaattgc catcaatgcg
     4741 ggtatttatg aagtagtccc tacttttatc aaagaatcag ataatgttta taatggcatg
     4801 tttgcgcgtt ggacaggttc taatctttat tctatggtat ctatgttttt tttggtggac
     4861 agggatggta agactatcgg cacagcgttg gagtggcaaa aagagttatc aacagatgat
     4921 ttaaataatt ctttagtttt taaagacttt gtggttaaga ctgttccaca agatttgttt
     4981 tttaatacta atgagccatt atttacaaca aaaggtaatg ttatccctgc aaattttgtt
     5041 ttgaaaactg ctcttggtga tagtatcaaa gtaacccgag gtgtattaag caaaaataat
     5101 atttcctctg gtgaagcagc ctatagattt gcatatggtt taacacaagc tcaggctgtc
     5161 gatgtttcgc agcttggtgc atggcaacgt gagtcagcaa caggtattag tcaaatttca
     5221 acagggactt tgagtttaca aaaaacacgt acgcctaata tgtatttaga cggtaatttt
     5281 tggcgtacta aagacaatat agtcacaggc gaaaaaccga tttttccttt acatttaaaa
     5341 ttaactttaa aagatagtaa cacctcaaca gcatgttcta gtacgggatg tttaataggt
     5401 gatataggta ttacgatttt agagaatggc aatattattt ctgatagaaa taatgactgt
     5461 tctgtggttg actccagtac attaaaagat acgctagata tgcaagagca gcgtttggga
     5521 tttgttgcca gtgtgttggt tgatagaacg caaaaagcaa ctagtgaatc attaattgcg
     5581 cctgttatgt tgattggtga ttgggcgaaa aaattaccat cttcagatgt ttggtataaa
     5641 ttgtatggct ttcatttagg ggctatttct ggtatgtctg gtgggccaaa agtacaaatt
     5701 gatgttgctc gggctacaac aggtatagta actattaaaa accaacaaga tgaacgggaa
     5761 aagttaggcg aagtcgcttt atgggcaaat tatgttaatt acttaaagta cttaagtgag
     5821 tcaaataccg aaaagaaaaa agaattaggt tatgcttctt taggagcagt aaccaacatt
     5881 caaactcaag catgctataa ccctcaaccc aaaatctaaa cctaaaaaag ccgctgattt
     5941 cagcggcttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaacgtctg
     6061 cgatagagtg ggcggctctc atcaacagaa gcttgataac tttcgctaaa gttatttaag
     6121 ccttgtaaag caacatgaat atctttatcg gcacgcacgg caaaactatt aaagccacaa
     6181 cgttttaagt aaaacaagtt gtctttaaac acatcacctg ttgcgcgtaa ttcacctgta
     6241 aagccaaaac gttgacgtaa taacgtagca tatgaatagc cacgaccatc ggcaaaagca
     6301 ggaaaatcta aagcgatcat atctaattgc ttaacgtcct caaccagcac ttcggggtgt
     6361 tggtctggtt ttaacaacac ggcttttttg tatggatggg caaggatggc ctcacgttgt
     6421 tgttgccaaa catcaaggct aacaacaata tcttgagcag gtaaagcacc tgcttcagta
     6481 actaattgat aggtatcagc gacgatagca ccgtctttaa taatattagg catagacgcg
     6541 ctccttaaat ttagctaaac caatgcggcg ataagtatct aagaaacgtt cgttttcttg
     6601 acggttatct aaatacacat tcaaaatttc ttcaatcaca tcggccattt cttcggcata
     6661 gaaagaaggg cctaagatgt cacctaaacg ggtgtcatgg ttgctatttc cacctaaaga
     6721 aacttgatag tattcttcgc cttttttatc gacgcctaaa ataccaatat gaccaacatg
     6781 gtgatgacca caagcattca tacagcccga aatattaata tctaaatggc caatattata
     6841 aacttggtca atgtcttcaa aacgacgttg aatttgttcg gcaataggaa tggatttagc
     6901 attagctaag gagcagaaat caccacctgg acagcaaata atattggtta agaagccaat
     6961 attagctgtc gcaaaatcta aggctgataa ttgttgccac aaatcaaata attgctcttg
     7021 acgcacatcg gctaaaacaa tgttttgttc gtgggtagtg cgtaattcac caaagctgta
     7081 ttggtcggct aaatcagcaa ccgtcaacat ttgttcgctg gtaatatcac ctggtgcaac
     7141 acctgtacgt tttaacgata acgttacaat acgatagcct gcttttttat gggcatgagt
     7201 attgtgttga taccataaag caaaagcttt gttgctgctt aaagcgtcag ttaacgcggt
     7261 tgggctatcc gttaagctct gataagcagg ctctgtaaag aaactggcta aacgtgcaaa
     7321 ctcttcagca ggaacaagca ttgcttcagt tttggtgtga gcaaattcgg cttcaacttt
     7381 ttgggcaaat acttcgggtg ttaaggcttt caccaaaatt ttgatacggg ctttatattt
     7441 gttgtcacga cgaccataca agttataaac acgtaaaacg gcatctaaat aagcaatcaa
     7501 ttcgttttgc ggtaagaatt cacgaataat cgagccaata attggcgtac ggcctaaacc
     7561 accaccaact aatactttgt agccaatttc gcctgcttca tttttgacga tataaatacc
     7621 aatatcatgc acggctgttg cggcgcgatc tgtatcaggt aaagcattaa ccgcgatttt
     7681 gaatttacgg ggtaaaaaag caaattcagg atggaaggtt gaccattgac gaattaattc
     7741 acaagttggg cgtggatcaa taatttcgcc tgccacaaca cccgcaaatt ggtcagtcgt
     7801 ggtattacga atacagttgc cgctggtttg aatagcgtgc atttgtactt tggccaagtc
     7861 agccaaaata tcaggcactc tggcaagttc tggccagtta tattgaatgt tttggcgagt
     7921 actgatgtgg gcataaccac ggtcataatc gcgggcaata ttagccaagg tgcgtaattg
     7981 tacactcgat aacattccgt aaggaatcgc aacccgtaac attggcgcat aacgttgtac
     8041 gtataaacca ttttgtaaac ggaaagggcg gaattggtct tcggttaatt cttgattgag
     8101 ataacgttga gtttggtcac gaaattgtgc cacacgttga tcgacaattt tttgatcgta
     8161 agcgtcgtat tgatacataa gccttgcact gtgcttggta aaaaaattgt gcgaagcata
     8221 tcaagacttt ctatataact aaaatacttt ttatctatat agacattagg ttttagtgat
     8281 aaggacttgc tctccagttg cagagggctg agcggagtcg aagcctatac tataaactgc
     8341 tgccgatatt ctttaggaga cactgcaaac caacgcttaa aggcacgacg gaagttggca
     8401 ctgtcatgat aattaagcaa agaggcaatc gcttcaacag acattgtact attgcgtaaa
     8461 tagtgactcg cttgttcgga taataatttt tcgcgaattt ccatataact actgccttct
     8521 ttggctaaat ggcgtgcaag cgtgcgtttg ctaataaata aagccgcagc agcatcttct
     8581 tcgctgagtg tgcctaaagg ctgagataac atcatttttt gtagttgata ggtggtgctg
     8641 cattt
//
LOCUS       DAOQMB010000052         8567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3273_length_8567_cov_15.746123, whole genome shotgun sequence.
ACCESSION   DAOQMB010000052 DAOQMB010000000
VERSION     DAOQMB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8567
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3273_length_8567_cov_15.746123"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLY05_03300"
     CDS             complement(<1..793)
                     /locus_tag="PLY05_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02360.1"
                     /translation="MLKQDMSPSDKFLENHPNLLNYTHNILSKRKYTFLFPRILEAHF
                     VAKRALESQAFINTGRILLILLFLIIVANVTLYYQHIVLQKNYQIIKQTYIPLSLAIT
                     FILFSPFINYVRSHFYYFMAPCSVFILYHIIILTLQYGGDYSDFVVYHLMMAIILMAF
                     GLRFVLPLFCIILTTAGLLGVIYAHIYNIPINFAKFSNYYILYCLVVMALTAISEWHE
                     RLAFLQGLLLDHQSQELNLLNKELERIAHEDALTGIANRRSFDVAA"
     gene            complement(802..2085)
                     /locus_tag="PLY05_03305"
     CDS             complement(802..2085)
                     /locus_tag="PLY05_03305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC02361.1"
                     /translation="MNLEGQLRSLLNQLSQDSSVATFAQNLLYTKPIFVNFRPDIENA
                     FLAKRTLEFSLIFRIASFLWLTLYLVILSGSYYHFPEIFKDKAYIIWQSAFLSAGIVI
                     FASCICCFITKTHAFIHQYIVAPAITLLLYQLLVGSVAHADNESGLYASYNIIIAMII
                     ALNSLRIVWQKGLFILVCSGLMAYATAFVNNWHIPLLPALHSFVLIGVVLTNITFFIE
                     RRERFAFLNEILVEIKSHELSRINRHLITIAREDALSGLANRRAFDDTLIIEWDRARR
                     EEQPLSLLFMDVDHFKLYNDTYGHGAGDDCLRQVASAIKKAVLRPADLAARYGGEEFV
                     VLLPATDALGAEEVAERILRTVDSLAIPHKRSLVSYHVTVSIGICTMLPSDKNSIAIF
                     VENADAALYKAKTSGRHCLKHYIEPPMLNNFYFKH"
     gene            complement(2122..3378)
                     /locus_tag="PLY05_03310"
     CDS             complement(2122..3378)
                     /locus_tag="PLY05_03310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC02362.1"
                     /translation="MLNVPPSSPSSTAEAPAQLISPKTLAYIHQLLNDKPLFFYFEND
                     IELRFYAKRAEEYRRLLSLGKLLFFVLYGIITFIALAIFPESVMAEEQFIFKYVLVPI
                     GLLLLLVLLLAEQPLFQRHYQWLATPISTLILYCVMLASVSNIDSTFAQHAAYDVMII
                     VTIISFGLRVQFPVCLLIVCLAAILTVLTTISLNWKIDWFKFTHFYGLGSTITLLIVA
                     LIERQERFAFLQELLVAHQTVELDRLNRALDKMSREDPLTGLANRRAFDDVLNREWER
                     ARREQQSIALLYMDVDFFKFYNDTYGHNIGDVCLRRVGQTLKQALRRPTDLAARYGGE
                     EFVVLLPNTNADGATDVAQRIIKHIDALALPHSRSKAASHVTLSIGITFTIPQKQQTL
                     TEFLAKADAALYKAKEHGRHQYQMEI"
     gene            3816..4697
                     /locus_tag="PLY05_03315"
     CDS             3816..4697
                     /locus_tag="PLY05_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC02363.1"
                     /translation="MTPQHFIPSAGVTLAAYTWGNPKTAKATIVLIHGYPDSAKIWQE
                     MAELLANDYYVISYDVRGSGESTIPKRIKQYDLSYLRQDLQAVIDTLAPKQKVHLVAH
                     DWGSIQTWEAVTEPSLQDRIASYTSASGPCLDHMGYWMRQRLSQPNAKAVGQLAKQFS
                     HSWYIWMFHLPFLAPNIWRFGGERLWPIMLEKVEGIKEEHPNPSQVKDGQYGVNLYRA
                     NIFKYVLAPRERYTTVPVQLLVPTNDHFMIEDIWDDLDQWVPNLTRSTIEAGHWLQLS
                     HAQQFAQAASQFIEKHR"
     gene            4971..5555
                     /locus_tag="PLY05_03320"
     CDS             4971..5555
                     /locus_tag="PLY05_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC02364.1"
                     /translation="MKRSVSLFLLLFAPCTWASFAEAISAYHQGQYNRAIHEFKQLAR
                     VGDVASQYNLAYMYARGLGVERDYGQAFTWFYAAAQRGDAESQFHLAGLYEQGLGIKQ
                     SHEKALNWYRQSALKGYLPAQITVAGLYGIGLGVEHDAAQSWTWLSVADSQRTQPMIA
                     EAKALLQEQLSPQQLQKAQHQAQAIIKQAWQTTN"
     gene            complement(5548..6738)
                     /gene="gltS"
                     /locus_tag="PLY05_03325"
     CDS             complement(5548..6738)
                     /gene="gltS"
                     /locus_tag="PLY05_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HNC02365.1"
                     /translation="MMISLEGIATLAIASFILLIGHFLKHQIGFLHRYNLPSPVIGGF
                     FAALTFLLCQQVGWQIKFDTTIKTPLMLVFFTSLGFAASLRLLKIGGKYVLVFLAISI
                     FAAILQNAVGASAAWAMGLPPLLGVLCGSVTLTGGPATGLAFAPQFEAVGIVGAESIA
                     VAAAMCGIIMGGVMGGPIGTLLIRRLQANHQPLTASETQTTIKLETTTDQHEQNLLIS
                     VGLLLIAMWLGSFISQFFTQVNITLPAYIGAMLAAATLRNLNDAFQWLKFDDQQIEQL
                     GGVALSYFLVLSLMGLDLSKLSAVALPLIVLILLQVVLMATCSMLLVFKFMGKDYEAA
                     VTATGFYGFMMGTTANAMANMDAFVKRYSPSPRAYLVVPIVGAFFIDFANTLLIQLCL
                     NLFS"
     assembly_gap    6859..6958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6959..7462
                     /locus_tag="PLY05_03330"
     CDS             <6959..7462
                     /locus_tag="PLY05_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004929490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02366.1"
                     /translation="GRGLVSTSFIDEEVRREFSGLPVGFYIQMVVLPSGPSFTAKVIA
                     GGHLELVKDFNGKADLTVKFKHLSHAFLVFSFQEGTARAFANDRMVADGEVSHAIRLV
                     RCLDKMEALILPKVVAQLAVKRYPTDLGLDEKLVKAVRIYRSIAQNFLNQDLPTIAPQ
                     LAKLVNK"
     gene            7501..>8567
                     /locus_tag="PLY05_03335"
     CDS             7501..>8567
                     /locus_tag="PLY05_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016803847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNC02367.1"
                     /translation="MAKSYYEFFCPVKVVAGHKALEHIPFELSTLQATRPMIITDKGV
                     RGAGLLEHVAAAFEGAESQVVAIFDDVPPDSSLQTVRRAAAMYRENNCDAIIAIGGGS
                     VIDTSKGVNILVTEGGDDLLKYSGAHNLPKRLKPFFVVPTTSGTGSEVTMVAVVSDTD
                     KGVKLPFTSYFLMPNAAILDSRMTLTLPPHITAMTGMDALTHAVEAYTCLAANPMSDA
                     YATAAIRKIGQNLMKVMENPQDADGRLELAQASTMAGVAFSNSMVGLVHALGHSLGAV
                     CHIPHGLCMSLFLPYVLEYNQEKNGERIGELLLYLAGEEVFAATPKAQRAAKAIQHIR
                     AMRDELYQRCKLPRTLTETGKV"
BASE COUNT         2714 a         1584 c         1721 g         2448 t
ORIGIN      
        1 ttgctgcaac atcaaaactg cggcgattag cgatgccagt taaagcgtct tcatgagcaa
       61 tacgttctaa ctctttgtta agtaagttta actcctgaga ttgatggtct aataataggc
      121 cttgtaaaaa tgcaaggcgt tcatgccatt cgctaattgc ggtaagtgcc atcacaacaa
      181 ggcaatacaa aatataataa ttagaaaatt tagcaaaatt aatgggaata ttataaatgt
      241 gagcgtagat tacacctaat aaaccagcag tagttaaaat aatacaaaat aacggtaata
      301 caaaacgtaa accaaaggcc attaaaataa tagccatcat taagtggtat acgacaaaat
      361 cgctgtaatc accgccatat tgcagtgtta gtataataat atgatataaa ataaaaacgg
      421 agcaaggcgc cataaaataa taaaaatggc tacgaacata attaataaat ggcgagaata
      481 aaataaatgt aatagctaga cttagaggaa tataagtttg tttgattatt tgataatttt
      541 tttgcaaaac aatatgttga taatataaag ttacattagc aacgatgatt aaaaataata
      601 aaattaacaa aatacgacct gtatttataa aggcttgact ttctaatgca cgtttggcaa
      661 caaagtgagc ttccaaaata cgaggaaata aaaaagtata tttgcgcttg ctgagaatat
      721 tatgtgtata gtttaataaa tttggatgat tttctaaaaa tttatcacta ggagacatat
      781 cttgtttcag catagtattc cttagtgttt aaagtaaaaa ttattaagca taggtggttc
      841 aatatagtgt tttaaacaat gtcttccaga tgttttggct ttgtataagg cagcatcggc
      901 attttcgaca aatatggcaa tactattttt atcagagggt agcatggtac aaataccaat
      961 actcacggtg acatggtatg acactaatga acgtttatgg ggtatagcta ggctatcaac
     1021 ggtacgtaaa atacgctccg caacttcttc cgctcccaag gcatcggttg caggtaataa
     1081 gacaacaaat tcttcacctc catagcgtgc ggctaaatca gcaggacgaa gaaccgcttt
     1141 tttgatggca ctagcgactt gtcgtaaaca atcatcaccc gcaccgtgac cataggtgtc
     1201 gttataaagt ttaaaatgat caacatccat gaacaataaa gataatggct gttcttctcg
     1261 gcgtgctcta tcccattcaa taatcaaggt gtcgtcaaat gctcgacgat tggctaaacc
     1321 tgaaagtgca tcttcacgcg caatggtaat taagtggcga ttaatgcggg agagttcgtg
     1381 acttttgatt tcgactaaaa tttcatttaa aaaagcaaag cgttcacggc gctcaataaa
     1441 aaaagtaata ttagttaaga caacaccgat caatacaaaa ctatgtaagg caggtaatag
     1501 aggaatatgc caattattga caaaagctgt cgcataggcc attaagccgc tacacactaa
     1561 aataaatagc cccttttgcc aaacaattct taagctattt aaggcaataa tcatggcgat
     1621 aataatatta taagaggcat atagccccga ttcattgtca gcatgggcaa cactaccaac
     1681 taaaagttga taaaggagta gagtgattgc gggtgctaca atatattgat gaataaacgc
     1741 atgagtctta gtaataaaac agcaaatgca actagcaaaa atgacaattc ctgcacttaa
     1801 aaaggcactt tgccaaataa tataagcttt atctttgaat atttcaggga aatgataata
     1861 agagcctgac aagataacaa ggtataaggt taaccacaaa aaactagcaa tacgaaaaat
     1921 gaggctaaac tctaaggtac gttttgctaa aaaagcattt tcgatgtcag gacgaaaatt
     1981 gacaaaaatg ggtttggtat agagcaaatt ttgggcaaaa gtagccactg agctatcttg
     2041 cgataactga ttcagtaaag agcgtaactg accctctaga ttcatgatga taacaggggt
     2101 actgcagtta acatcatatc attaaatctc catttgatat tgatgacgac catgttcttt
     2161 agctttgtat agtgcggcat cagctttggc taaaaactca gtaagtgttt gttgtttttg
     2221 tggaatagta aatgtaatac ctatacttaa ggtgacatgt gaggcagctt tagatcgact
     2281 gtgtggtaaa gctagcgcat caatatgttt gataatgcgt tgagcaacat cagtagcacc
     2341 atctgcgtta gtattaggta acaatacaac aaactcttct ccgccataac gagcagctaa
     2401 atctgttggt ctccgtaagg cttgttttag cgtttgccct acacgacgta aacaaacatc
     2461 gccgatatta tgaccatagg tgtcattgta gaatttgaag aagtcaacgt ccatatatag
     2521 taatgcaata ctttgttgtt cccgacgagc gcgctcccat tcccgattta agacatcatc
     2581 aaaagcccgt ctattggcta aacctgttaa tggatcttcg cgtgacattt tatcaagagc
     2641 ccgatttaga cgatcaagtt caactgtttg gtgagcaact aacaattctt gcaaaaatgc
     2701 aaaacgttct tggcgttcaa ttaaggcaac aatcagcaga gtgatggttg aacctaagcc
     2761 ataaaagtgg gtaaatttaa accaatcaat tttccaattt aagctgattg tggttaatac
     2821 cgttaaaata gcagccaaac agacaattaa taaacaaacg ggaaattgta cgcgtaagcc
     2881 aaaactaatg atcgtgacga tgatcattac atcgtaggca gcatgttgag caaaagtgct
     2941 atcaatattg ctgacactgg caagcatgac acagtacaag atgagcgtac tgattggcgt
     3001 ggcaagccat tggtagtgtc tttgaaaaag aggctgttct gccaagagta atactaataa
     3061 cagtaataac ccgattggaa ctaaaacata tttaaatata aattgctctt ctgccatgac
     3121 tgattcaggg aatatggcaa gggcaataaa ggtaataatg ccgtacagca caaaaaataa
     3181 aagcttacct aaagaaagca aacgacgata ttcttcggca cgtttggcat aaaaacgtaa
     3241 ttcgatatca ttttcaaaat aaaaaaacaa tggtttgtcg ttaagcaatt ggtgaatata
     3301 agctaaggtc ttcggcgaga taagctgagc aggtgcttct gctgtagaag atggcgaaga
     3361 cgggggaaca ttcaacacaa taaataccta tgttgttttt aaatccaaca gaccctacgg
     3421 atttacgcct tttgcaaact ttatatatgc aaactaggct aagctatgta agttcaatgt
     3481 cttgaacaaa cactgatttt tgttttttat tcatctgctt ttatcaataa atatccataa
     3541 actatgacta ttacgccata gtttagcaga ggataagata acaatctgat aaaaggtagt
     3601 cattgtcaga ttatcgctaa tatgtgttta aaaataaagc aaaatttatg agtaaaaatt
     3661 agcttctctc tctcaatact tcgtaggatg gagtgaggct ttctttctcg ctgtactagg
     3721 tttttaagtg cgtttgctct tatgctctat tgacttttac ctgattaaaa gcacatcatt
     3781 gactgttaca ttgattaatg tttttatggt gaattatgac tcctcagcat tttatccctt
     3841 ccgcaggtgt taccttagct gcttatacgt ggggtaatcc taaaacggcc aaagccacta
     3901 ttgtattgat tcatggttat cctgattcag caaaaatttg gcaagaaatg gcggagttat
     3961 tagccaacga ttattatgtg attagttatg atgtgcgtgg ctctggtgag tcaacgattc
     4021 ccaaacgcat taaacaatat gatttaagtt atttacgcca agatttacaa gcagtgattg
     4081 acaccttagc ccctaagcaa aaagtacatt tggttgccca tgattggggt tcaattcaaa
     4141 cgtgggaagc tgttactgaa cctagtttac aagatcgtat tgcttcctat accagtgcat
     4201 caggcccttg tttagatcat atgggatatt ggatgcgtca gcgtctcagc cagcctaatg
     4261 ccaaagcagt ggggcaatta gccaagcagt ttagccattc ttggtatatt tggatgttcc
     4321 acttaccctt tttagcacct aatatttggc gttttggtgg tgagcgtttg tggccaatta
     4381 tgctcgaaaa agtcgagggc attaaagaag agcatcccaa tccaagccaa gtcaaagatg
     4441 gtcagtatgg tgtaaacttg tatcgtgcca atatttttaa atatgtttta gcaccgcgtg
     4501 agcgttatac aaccgtgcct gtacaattat tagtaccaac gaatgatcat tttatgattg
     4561 aagatatttg ggatgattta gatcaatggg tacctaattt aacacggtca accattgagg
     4621 caggacattg gttgcaacta agccacgcgc agcaatttgc tcaagcggcc agccagttta
     4681 ttgaaaagca tcgctaagca gacgctattg cgttttaaga ttgatcgttt tatcagggaa
     4741 agcagatgct ttcccttttc ttttgcaaat taagtgtttg aattttggcc atttgctata
     4801 acacaccttt atattagcgt gataatatca ggcgacttaa agctaaataa ctaaaaaatt
     4861 tagtgataac aataggcaag gaaagtacag ctaattaatt atccattaag caggtaagtc
     4921 ctaactttta gctgatgcca taaagcataa atgtttgttg cgtggtcaat atgaagcgca
     4981 gtgtttcgtt atttttatta ttatttgcac cctgcacatg ggctagcttt gctgaggcta
     5041 tttctgccta tcatcaaggg caatataacc gtgctattca tgagtttaaa caattagccc
     5101 gtgtcggtga tgttgcgtcc caatataact tggcttatat gtatgcacgt ggattaggcg
     5161 ttgaacgcga ttatggtcaa gcctttacgt ggttttatgc cgcagcacaa cgtggagatg
     5221 cggagtcaca attccacttg gctggtttgt atgagcaagg tttgggaatt aaacaaagcc
     5281 acgaaaaagc cctaaactgg tatcgccaat cggcattaaa aggctatttg cctgcacaaa
     5341 ttaccgtggc aggtttatat ggtattggtt taggggtaga gcatgatgcg gctcaatcgt
     5401 ggacttggtt gagcgtcgca gacagccaac gcacacagcc aatgattgca gaagctaagg
     5461 ctttactaca agagcagcta agtccgcagc aattgcaaaa agcccaacat caagcccaag
     5521 ccatcataaa acaagcatgg caaacgacta actgaatagg tttaaacaaa gctgaatcag
     5581 taaggtattg gcaaaatcaa taaaaaaagc acccacaata ggcacgacta aataggcgcg
     5641 gggcgaggga ctataacgtt taacaaaagc atccatattg gccatagcgt tcgccgttgt
     5701 gcccatcata aatccataaa aacctgtggc tgtcacagct gcttcatagt ctttgcccat
     5761 aaatttaaag actaaaagca tgctacaagt agccattaat acaacctgta ataaaattaa
     5821 aacaattaac ggtaaagcaa ccgcactgag tttagataaa tctaagccca tcagtgataa
     5881 cactaaaaaa tatgataaag cgacaccgcc aagttgttct atttgttgat catcaaactt
     5941 tagccattga aaagcatcat ttaaattgcg taatgttgcg gctgctaaca ttgcaccaat
     6001 ataagcaggt agtgtgatgt ttacttgggt aaaaaattga ctaataaaac tacctagcca
     6061 catagcaatc aataataaac caacactaat aagtaagttt tgttcatgct gatcggttgt
     6121 tgtctctaat ttaattgtgg tttgtgtttc gctggcggtt aatggttgat gattcgcttg
     6181 caagcgacga atgaggagtg tgccaattgg cccgcccatc accccaccca taataatgcc
     6241 acacatcgct gcagcgacgg caatcgattc cgcaccgacg attcccactg cttcaaattg
     6301 tggggcaaac gccaagcctg ttgcaggccc gccagttaag gtcactgaac cacacaatac
     6361 tcccaacaat ggtggtaaac ccattgccca tgcagcactt gcaccaacag cattttgcaa
     6421 tatcgcagca aaaatactaa ttgctaaaaa gacgagtacg tatttgccac cgatttttaa
     6481 cagtcttaaa ctcgctgcaa aacctaaact ggtaaaaaag accagcatca aaggtgtttt
     6541 gatcgtggtg tcaaatttta tttgccagcc cacttgttga cacaataaaa aggttagggc
     6601 agcaaaaaag ccaccaatga caggactagg taagttgtaa cgatgtaaaa agccaatctg
     6661 gtgtttgagg aaatggccaa ttaataaaat aaaactggca atcgctaaag tggcgatccc
     6721 ctctaaagaa atcatcatcg ttttaatcct taaaatcatc tatccacatt atccactctt
     6781 gctctaaggc tttgctagtg atactgtcag acacctttgt ttaggattcc aatcatagtc
     6841 attgcatatg gtcgatgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     6961 tcgaggttta gtgtctacca gttttattga tgaagaagta cgacgcgaat tttcgggttt
     7021 gcccgttggt ttttatattc agatggtcgt attgccttct ggccccagct ttaccgctaa
     7081 agtgattgca ggtggccatt tagagttggt caaagacttt aatggtaaag ctgatttaac
     7141 ggtgaagttt aagcatttat cccatgcctt tttagtcttt agttttcaag aagggactgc
     7201 acgtgcgttt gcaaatgatc gtatggtagc agatggtgaa gtctctcatg ccattcgttt
     7261 agtgcgttgt ttagacaaaa tggaagcctt aattctgcct aaagtggttg cccaattagc
     7321 agtgaaacgt tatccaactg atcttggcct agacgaaaaa ttagtgaaag cggtgcgcat
     7381 ttatcgttct attgctcaga attttttaaa tcaggactta cctacgattg ccccgcaatt
     7441 agcaaaatta gttaataagt aatcaaaaac aaggtaatcc ccatctatag gagttaaaac
     7501 atggctaagt cgtattatga atttttttgt cctgtaaagg ttgttgcagg tcacaaagcc
     7561 ttagagcata tcccctttga gttatcgacc ttacaagcca cacgcccgat gattatcacc
     7621 gacaaaggtg tgcgtggtgc tggtttgtta gagcatgttg ctgctgcttt tgaaggcgca
     7681 gagagccaag tcgttgctat ttttgatgat gtgccgcccg atagtagttt acaaactgtg
     7741 cgtagagcag ccgcaatgta tcgtgaaaac aactgtgatg cgattattgc cattggtggc
     7801 ggttcggtga ttgacactag taaaggggtg aatattttag tcactgaagg tggtgatgat
     7861 ttattaaaat attctggcgc acacaactta cctaagcgtt taaaaccatt ttttgtagtg
     7921 ccaacgacat caggcacagg ttctgaagtg acaatggttg ccgttgtttc tgatacagac
     7981 aaaggtgtta agttaccgtt tacgtcttat ttcttgatgc caaacgcggc gattttagat
     8041 tcacgcatga ctttaacctt gccaccgcat attacggcca tgacaggtat ggatgcatta
     8101 actcatgcag ttgaagctta tacctgttta gcagctaatc cgatgagtga tgcttatgct
     8161 actgctgcca ttcgcaaaat tggccaaaac ttaatgaagg tcatggaaaa tcctcaagat
     8221 gccgatggcc gtttagaatt agctcaagcc tcgacaatgg caggtgtggc attctctaat
     8281 tccatggttg gtttagtaca tgctttaggc cattcattag gtgcggtgtg tcatattcct
     8341 catggcttgt gcatgagctt attcttgcct tatgttttag agtacaacca agagaaaaat
     8401 ggcgagcgta ttggtgagtt gttattgtac ttagcgggtg aagaagtgtt tgctgccaca
     8461 ccaaaagcac aacgtgcagc taaagcaatt caacatattc gtgcaatgcg tgatgaattg
     8521 taccaacgtt gcaaattacc acgtacctta accgaaactg gtaaagt
//
LOCUS       DAOQMB010000053         8540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3295_length_8540_cov_16.353801, whole genome shotgun sequence.
ACCESSION   DAOQMB010000053 DAOQMB010000000
VERSION     DAOQMB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3295_length_8540_cov_16.353801"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..1115)
                     /gene="ubiA"
                     /locus_tag="PLY05_03340"
     CDS             complement(243..1115)
                     /gene="ubiA"
                     /locus_tag="PLY05_03340"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNC02368.1"
                     /translation="MTSFLQQRLPLYAEVMRLHRPIGIYLLLWPTLWAVWLASNGKPD
                     ASIVLIFILGVVFMRSAGCVINDWADRNFDGHVARTKDRPIASGRLPAREALYLFVFL
                     VACSALLLPFLNLQTFFWSFGALALASLYPFMKRFIYLPQLVLGAAFSWAIPMAFVAQ
                     NHNPDLICWLLYAANLIWTVAYDTQYAMADRDDDVKIGVKSTAILFGRFDLIIISLLQ
                     GLFILLLALIGYLQHFGLIWAITLGICGGLFIWQYGHCNDRQAQHCTESFLHNHKVGM
                     VIFLGLVLSLVFKI"
     gene            complement(1112..1630)
                     /locus_tag="PLY05_03345"
     CDS             complement(1112..1630)
                     /locus_tag="PLY05_03345"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HNC02369.1"
                     /translation="MPLHPRWLTPTRYWQAQLRSPYKAWLLSQGSLTAYLVALSHGHF
                     RVKVQHEAWQKPTLDERLSLKLPHQHYAWVREVALLGHNQQWVTARSIIPQHTLVGRG
                     RRLKYLGSRSLGSLLFKGGKRGQLVICSPNSQHHHWTRRSLFYYANRPLLVQEKFLPA
                     LFEAAQQQQICV"
     gene            1810..2280
                     /locus_tag="PLY05_03350"
     CDS             1810..2280
                     /locus_tag="PLY05_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNC02370.1"
                     /translation="MSNIPATLTEKNLEGAFAGESMAFMKYSYFAKLARAMGDEATAQ
                     AFEETAKQEIQHAFGHLDLLYPAESLNPAKMLEIAIAGETYEYTEMYPKFKETAIAEG
                     KADAIQEFDEQIAESKEHAEAFAKVLEKAAKRFAALAKVEERHANHYRQRLAAL"
     gene            2451..2615
                     /locus_tag="PLY05_03355"
     CDS             2451..2615
                     /locus_tag="PLY05_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004972007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNC02371.1"
                     /translation="MKKWQCIVCGFIYDEAVGMPWDGVPAGTAWADVPADWVCPDCGV
                     GKDDFEMVEI"
     gene            2761..3915
                     /locus_tag="PLY05_03360"
     CDS             2761..3915
                     /locus_tag="PLY05_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC02372.1"
                     /translation="MALVIIGTGLAGYNLAREWRKLNPEKALVIISRDDGRNYSKPML
                     STGYTKGKTADQLAMQSAAQMSEQLKAEIRTFASVEAIDTQAKTVTVDGVSIAYEQLV
                     LALGADPFQPPLAGDGLDQVYTVNDLMDYAKFQQAAAGKKNVIVIGGGLIGCEYANDL
                     TNGGYAVQLIEPVGRVLPALLPPVASQAVGNALEGLGVKFHFGVSVQAVYKDGEGVRA
                     VLSDGSEIQGDIVLSAIGLRPRISLAKEAGLAVNRGIVVDKTLATSQADIYALGDCAE
                     VEGLVLPYVLPLMAGARALAKTLSGQTTAISYPVMPVQVKTPVCAVVVAPTFPSMQGE
                     WTVDSQDGNNVKALFKNTEGQLLAYALTGSFAGDAALKAELAKQVPAWLV"
     gene            4040..4516
                     /locus_tag="PLY05_03365"
     CDS             4040..4516
                     /locus_tag="PLY05_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02373.1"
                     /translation="MTEISVRHTFQGSLDEVFEAVINADRYPYVSRLFHVERLCLAKS
                     GEKDGVGTQREVELGVVWFKEEFTLFDRPNRVDYKIFETRPYFDHQHGSFIFKKVGHK
                     LIEVTWESCFVIPSQTIPPKILSTVSGWMVKAAFKSALMILDRKLQEKRQPKRLAK"
     gene            4635..>5456
                     /locus_tag="PLY05_03370"
     CDS             4635..>5456
                     /locus_tag="PLY05_03370"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HNC02374.1"
                     /translation="MFARLNYRYTIMRDLMVVYVFLMLVLMSCAAWVHADDSEAVQTE
                     SPYFFINSNDPQTDRLPLKSTKVDVHIVGMIADVTIKQVYRNEGKRTIEARYVFPSSS
                     QSAVYAMHVRLQDRVINARIREKQQAKQEYETAKSEGKTAALLEQQRPNVFEMNVANI
                     LPNDEVVVELRYTELLSPRDMLYSFVFPTVVGPRYNSPQSPAAAEKWVKNPTLPQGIA
                     SESRFNIKVTMDSPIPVQMVSSPSHSLRSDGLGSKHVDVMLGDNQIANNRDFVLRY"
     assembly_gap    5457..5556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5557..6997
                     /locus_tag="PLY05_03375"
     CDS             <5557..6997
                     /locus_tag="PLY05_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC02375.1"
                     /translation="VMLGDNQIANNRDFVLRYIASESRFNIKVTMDSPIPVQMVSSPS
                     HSLRSDGLGSKHVDVMLGDNQIANNRDFVLRYRLAGEKIQSGLLLYRGKDENFFLGMV
                     EPPKQVATAQIQPRDYIFVVDVSGSMHGHPLDITKQLLRNLIGGLRSTDTFNVMLFSG
                     NSSMLAPQSLPATASNIQQAIDTIDQQQGGGSTEIVPALRKITELPKSPDVSRSVIVV
                     TDGYVTVEDEVFKLIRQQLHQANVFAFGIGSSVNRVLIEGMARAGQGEPFIVTKPEEA
                     DEQAEHLRKMIQAPVLTQVTAKLTGVDAYDIQPTVLADVLSGRPVMVMGKWRSRRDKE
                     AEATLVVTGRSTEGEYQQQVSAKSDNSEDNSALRLLWARQKIAELSDQEALEGNGSYK
                     QAITDVALKYGLLSAYTSFVAVDEVVRNVNPEDSSTVTQPVPLPEGVSNLAVGSGIPS
                     TPEPNLWLALALVFLMLVIMKKTYRRKII"
     gene            7110..8468
                     /gene="xrtQ"
                     /locus_tag="PLY05_03380"
     CDS             7110..8468
                     /gene="xrtQ"
                     /locus_tag="PLY05_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase Q"
                     /protein_id="HNC02376.1"
                     /translation="MFNNRLSMTACLWLCLISQLLALWPQGQWIAARMQDGSDQPLGI
                     IAVLSLAALLITQQSAWQAPKTRFLLMAVVLQLAACGAWLMLPPLLSSLLAVLAISML
                     IAAFLPQNSPYLPIFGLAVLSLPLLSSLQFYAGFPLRVITAQCSAWILQLWGNQVERI
                     GTNLQINGQLVMVDAPCSGVQLAWMVYFFACVIALYRQIADKHFLRCWPLLGVLVLLG
                     NIIRNTCLVVLETRPQGLSDSLHQSMGLVILVMVCSVVAWLLSKQPTPEKQEKSMVLA
                     SPAWLFPLVMLCMMLSGAVSWSKQSATTQANPKYIEWPQQWDGKPLQPLALSVVENRF
                     AANFAGHIGRFSDGQRQIVLRYVEQPTRMLHPASDCYKGLGYHIQHEQLEYDDQQRVW
                     RCFYATKQGQIVRVCERIVDKQGKVFTDTSSWYWQAELGHSQGVWWAITTAEVVAMVA
                     ER"
BASE COUNT         2497 a         1620 c         1907 g         2416 t
ORIGIN      
        1 gtaaagcggc gttattgaaa aacaaatagt ggtgtttttt taatcgcaga caagaattaa
       61 actgtataag ataaggagga ggttgaggcg tgataagctt gtattcaaat aagccgtata
      121 ttaaagccaa tactaacaat aagtagcacg ggttgaggtg cgaaatttat gatagttgtt
      181 ttcgtgggtt tccttcgtca actcaagtta ggattaggtt tttatttgta cgaaagttta
      241 agttatatct taaacaccaa acttaacacc aaacctaaaa aaatcaccat acccacttta
      301 tgattatgca aaaatgactc ggtgcagtgt tgggcttgtc gatcattgca atggccatat
      361 tgccaaataa acaagccacc acaaatgcct aaagtaattg cccaaattaa gccaaagtgt
      421 tgcaaataac caattaaggc cagcaataaa ataaacaaac cttgtaataa actaatgata
      481 attaagtcaa aacgaccaaa cagaatcgct gttgatttta cgccaatttt tacatcatca
      541 tcccgatcag ccattgcata ttgggtatca taagccactg tccaaattaa atttgctgca
      601 tataacaacc aacaaattaa atcgggatta tgattttgtg ccacaaaagc catgggaatt
      661 gcccacgaaa aagccgcacc caagactaat tgcggtaaat aaataaaccg tttcataaac
      721 ggatataaac tggctaaagc taatgcacca aacgaccaaa aaaaggtttg tagatttaaa
      781 aacggcaata acaatgcaga acaggcaacc aaaaatacaa acaaatacaa agcttcacgc
      841 gcaggcaaac gtccactggc aattgggcgg tctttggtgc gtgctacatg accatcaaaa
      901 ttgcgatcag cccagtcatt aatgacacat cctgccgaac gcataaacac cacgcccaag
      961 ataaaaatca acacaatcga agcatcaggt ttaccattac tcgctaacca aaccgcccat
     1021 aaggttggcc ataacagcaa ataaatacca ataggacgat gcaatctcat gacttcggca
     1081 tagagcggta aacgttgttg caaaaatgat gtcatacaca aatctgctgt tgctgagcag
     1141 cctcaaataa agcaggtaaa aacttttcct gtaccaataa aggacgattc gcataataaa
     1201 acaacgaacg ccgagtccaa tggtggtgtt gtgagttggg tgaacaaatc actaattggc
     1261 cacgtttgcc ccctttaaac aacaaactgc ctaaagagcg cgaacccaaa tactttaagc
     1321 gtctgcctcg acccactaag gtgtgttggg ggataatgct tcgtgcagtg acccattgtt
     1381 gattgtgacc taacaacgcg acttcacgca cccacgcata gtgttgatga ggtaatttta
     1441 ggctcaatcg ttcatctaag gtgggtttct gccacgcctc atgttgtact tttaccctaa
     1501 aatgaccatg acttaaagcc actaaataag cagttaaaga gccttgtgac aaaagccacg
     1561 ctttgtacgg tgagcgaagt tgcgcttgcc aatagcgagt tggcgttaac cagcgaggat
     1621 gaagcggcat acaatgtatc taagaggtca ttgataacaa cgaattatac tcagtttaac
     1681 ggctatctgg cctatcaata ttaatagttt ttagctatta aaaaatacat tttatttaat
     1741 ttgataaaag tttctttagc gtttaatctt aaactcaaca agacgaaaat tagatttgga
     1801 gagtgcaaga tgagtaatat tccagccaca ttaaccgaaa agaatttaga aggtgcattt
     1861 gcaggcgaat caatggcatt tatgaagtat agctattttg ccaaacttgc gcgggcaatg
     1921 ggtgatgaag ctaccgcaca agcatttgag gaaacagcaa aacaagagat ccaacatgct
     1981 tttggtcatt tggatttgtt gtatcccgca gaaagcctaa acccagcaaa aatgctcgaa
     2041 attgccattg cgggtgaaac ctatgaatac accgaaatgt atcctaagtt taaagaaacg
     2101 gcgattgctg aaggtaaagc cgacgctatt caagagtttg atgagcaaat tgctgaatca
     2161 aaagaacatg cggaggcttt tgctaaggtg ttagaaaaag cagcaaaacg ttttgccgct
     2221 ttggctaaag tggaagagcg tcatgctaat cactatcgcc aacgtttggc tgccctttaa
     2281 atttttctgt tattaatagc ttttttggcg tttggcgtag ccagtcaagc aaaaaaatat
     2341 gttaaacttt acattaaaca atgtgacagt ttagcagaga ttgcaaataa agttgtttat
     2401 gcgtaaggta ggcagcttta cgatttatcc cgcttagcaa gaggtttaac atgaagaagt
     2461 ggcaatgtat tgtttgtggt tttatttatg atgaagctgt cggtatgcct tgggatggcg
     2521 tgccagcagg aacagcatgg gctgacgtgc cagccgattg ggtttgccca gattgtggcg
     2581 tgggtaaaga tgattttgaa atggtagaaa tttaattcta ttgttcaaaa aagatcaaag
     2641 ggcgcacaaa gttgcgcctt ttttatggtt tgattttgtt gctattttta gccctcactc
     2701 cgaccctatc cctttgggag aaagttagag tgagggttgt tattttattg aggaaaaatc
     2761 atggcgttag tcattattgg tactggttta gcaggttata acttggcgcg tgaatggcgc
     2821 aaactgaacc ccgaaaaagc attggtgatt attagccgtg atgatggccg taattactca
     2881 aagcctatgt tgtcaactgg ttacaccaaa ggcaaaactg ccgatcaatt agccatgcaa
     2941 agcgcggcgc aaatgtccga gcagcttaaa gccgaaatca gaacctttgc tagcgtagaa
     3001 gccattgata ctcaagcaaa aacagtgact gttgatggtg taagcattgc ctatgagcaa
     3061 ttagtactgg cgctcggtgc tgatccattt caacctcctt tagcaggtga tggtttagac
     3121 caagtttata ccgttaatga tttaatggat tatgccaagt ttcaacaagc cgccgcaggc
     3181 aaaaagaatg taattgtgat tggtggcggt ttaattggtt gtgaatatgc caacgatttg
     3241 actaatggcg gttatgcagt gcaattaatt gagccagttg gtcgggtgtt gccagcctta
     3301 ttaccgcctg tggcttcgca agctgtgggt aatgccttag aaggtttggg cgttaaattt
     3361 cattttggcg taagcgttca ggctgtttat aaagacggtg agggtgttcg tgctgtgtta
     3421 tccgatggca gcgaaatcca aggtgatatc gtattgtcag cgattggttt gcgtccacgc
     3481 attagccttg ccaaagaagc agggttggcg gttaatcggg gtattgtcgt tgataaaacc
     3541 ttagccacta gccaagccga tatttatgca ttgggcgatt gtgccgaagt cgaaggttta
     3601 gtgttgccct atgtcttgcc attaatggca ggtgcgcgtg cattagctaa aactttatca
     3661 ggtcaaacaa ccgcgattag ttatcctgta atgccagtgc aagttaaaac gcctgtttgt
     3721 gcggtggtag ttgcacccac tttcccaagt atgcaaggtg agtggacagt tgatagccaa
     3781 gatggcaaca acgttaaagc gttgtttaaa aataccgagg gtcaattatt ggcttatgcc
     3841 ttaacagggt catttgcggg tgatgcagcc ttaaaagctg aattagccaa acaagttcct
     3901 gcttggttag tgtaaaatct caataaggat tttgctgctt tagcgctgtt taacaaggaa
     3961 ataagcttcc ctctcctgta ggagagggct actgcgagcg agcaaaacgt taatttttac
     4021 taactaagag gaaaagcaca tgacggaaat atcagtaaga catacttttc agggttcttt
     4081 ggatgaagta tttgaagcag tgattaatgc ggatcgttat ccctatgtgt ctcgtttatt
     4141 tcatgtggag cgtttgtgtt tagcaaaatc gggtgaaaaa gatggtgtag gcacacagcg
     4201 tgaagtggaa ttaggcgtag tttggtttaa ggaagaattt accctgtttg atcgacccaa
     4261 tcgtgttgat tataaaattt ttgaaacacg cccttatttt gaccatcaac atggtagttt
     4321 tatcttcaaa aaagtggggc ataaattaat tgaagtgact tgggagtctt gttttgtcat
     4381 cccttcacaa acgattcctc caaaaatttt atctacagta tcgggttgga tggttaaagc
     4441 agcgtttaaa tcggcattaa tgattttgga tagaaaacta caagaaaagc ggcaaccaaa
     4501 acgcttagca aaataaataa gcgaaaacag cgtcaattca aaacactctt cactcaaact
     4561 tcacacaatc ccccctgtgt gttcaatcgc tgtttataag ctaaggcaag ctaattttaa
     4621 aaaaggagct tgccatgttt gcgcgtctca attatcgcta caccatcatg cgtgatctga
     4681 tggtagttta tgttttttta atgttggttt taatgtcttg tgcagcttgg gttcatgccg
     4741 atgactccga agccgttcaa acagaaagcc cttatttttt tattaatagt aatgatccac
     4801 aaaccgatcg tttaccacta aaatcaacca aagttgatgt ccatattgtt ggcatgattg
     4861 ccgatgtcac cattaaacaa gtgtatcgta acgaaggcaa acgcaccatt gaagcccgtt
     4921 atgtgtttcc aagttctagt caatcagccg tttatgcaat gcatgtgcgt ttacaagata
     4981 gagtaattaa tgcgcgtatc cgtgaaaaac aacaagctaa acaagaatat gaaacggcta
     5041 aatccgaggg aaaaacggcg gcattattag aacaacagcg tccaaatgtc tttgagatga
     5101 atgtcgctaa tattttgcct aatgatgagg ttgtcgttga gttacgttat accgaattgt
     5161 taagccctcg tgatatgttg tatagctttg tttttccaac cgtggttggc ccgcgttata
     5221 acagtccaca aagcccagcg gctgccgaaa aatgggtaaa aaatcccacc ttgccacaag
     5281 gcattgcctc agaaagtcgc tttaatatta aagtgaccat ggatagtcct attcctgtac
     5341 aaatggtgtc atcaccctcg cattctttac gcagtgatgg tctgggtagc aaacacgttg
     5401 atgtgatgtt gggtgataat caaatcgcta ataatagaga ttttgtgttg cgctatnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgtg atgttgggtg ataatcaaat
     5581 cgctaataat agagattttg tgttgcgcta tattgcctca gaaagtcgct ttaatattaa
     5641 agtgaccatg gatagtccta ttcctgtaca aatggtgtca tcaccctcgc attctttacg
     5701 cagtgatggt ctgggtagca aacacgttga tgtgatgttg ggtgataatc aaatcgctaa
     5761 taatagagat tttgtgttgc gctatcgttt ggcaggtgaa aagattcagt caggattgtt
     5821 gttataccgt ggcaaggatg aaaacttttt tttaggtatg gtagagccgc caaaacaagt
     5881 cgctactgcc caaattcagc cccgtgatta tatttttgtc gttgatgtct cggggtcaat
     5941 gcacggtcat ccactggata ttaccaaaca attattacgc aacctaattg gcggattacg
     6001 ttcaacagat accttcaatg tgatgttgtt ttcgggcaat agctcaatgt tagccccaca
     6061 atctttacct gctactgcaa gcaatattca acaagccata gatactattg atcaacaaca
     6121 gggtggcggc agcaccgaaa ttgtgcctgc tttgcgcaaa atcaccgaat tacccaaatc
     6181 gcctgatgtg tcgcgcagtg tgattgtggt gactgatggt tatgtgacgg ttgaagatga
     6241 agtatttaaa ttaattcgtc agcaattaca tcaagccaat gtctttgcct ttggtattgg
     6301 tagcagtgtt aatcgggtgt tgattgaagg tatggcgcgt gcagggcagg gtgaaccctt
     6361 tattgtcact aaacctgaag aagccgacga acaagccgaa catttacgca aaatgattca
     6421 agcccctgtc ttaacccaag tcaccgctaa gctcacaggc gttgacgctt atgatattca
     6481 acccaccgtg ttagctgatg tgttatctgg ccgtcctgtc atggtcatgg ggaaatggcg
     6541 tagtcgtcgt gataaagagg ctgaagcaac cttagtggtt acaggtcgta gtactgaggg
     6601 tgaatatcaa caacaagtgt ctgcaaaaag tgacaatagc gaagataaca gtgccttacg
     6661 tttattatgg gcaagacaaa aaatagccga attgtccgat caagaagcct tagaaggtaa
     6721 tggttcttat aaacaagcga ttactgatgt ggcgttaaag tatggtttgt tatctgccta
     6781 caccagtttt gtggctgttg atgaagtggt acgcaatgtt aacccagaag atagcagcac
     6841 cgtcacccag cctgttccac taccagaagg cgtcagtaat ttggcggttg gttcaggcat
     6901 tccttcaaca cctgaaccca atttatggtt agctttagcc ttagtcttct tgatgttagt
     6961 catcatgaaa aagacttatc gtagaaaaat tatttaaatt caaatagata attggtaacg
     7021 gcgtaagtcc taatagtcca agaacaagct accactcagg gctgagacat gtccgtgtaa
     7081 tgtctcagct cccaacaaag aagttcatca tgtttaataa tcgcctatcc atgaccgctt
     7141 gtttatggct gtgtttgatt agtcaattgc tggccttatg gccgcaaggc caatggatag
     7201 cggccagaat gcaagatggt tctgaccaac cgttgggaat tattgccgtg ctgagtttgg
     7261 ctgcgttact catcactcag caatccgcat ggcaagcacc aaaaacaaga tttttgctga
     7321 tggcggttgt tttgcaattg gcagcttgtg gcgcatggtt aatgttgcca ccattgctgt
     7381 cttctttatt ggctgtttta gcaatttcga tgctaatagc ggcttttttg ccacaaaata
     7441 gcccttattt accgattttt ggcttggcag tgttaagcct acccttatta tcatcgttac
     7501 agttttatgc aggctttcca ttaagagtca ttactgctca atgtagcgca tggattttac
     7561 aattatgggg taatcaagtt gagagaatag ggacgaatct acaaattaat ggccaattgg
     7621 tgatggttga tgcaccttgt tcgggagtac aactggcatg gatggtgtat ttttttgcct
     7681 gtgtcatcgc cctatatcga caaatagccg ataagcattt tttgcgttgt tggccgttat
     7741 taggggtatt ggtgttgttg ggcaatatta ttcgcaatac ttgtttggtg gttttagaaa
     7801 cacggccaca aggattaagc gattcattgc atcaaagtat gggtttagtc attttggtca
     7861 tggtttgtag tgtggtggca tggttgttaa gcaaacagcc gactccagaa aagcaagaaa
     7921 aatcaatggt cttggccagt ccagcatggc tttttccttt ggtcatgctg tgtatgatgt
     7981 tgtcgggtgc ggtgtcttgg tcaaaacaat cagcaacaac acaagccaat cctaaatata
     8041 tcgaatggcc acagcaatgg gatggcaagc cgttacaacc cttagcctta tcggtggtag
     8101 aaaatcgttt tgccgcaaat tttgcagggc atattggtcg ttttagtgat ggtcaacgac
     8161 aaattgtgtt gcgttatgtt gagcaaccga cacgaatgct gcatcctgcc agtgattgtt
     8221 ataaaggctt gggttatcac attcagcacg aacaattaga atatgatgat caacaacggg
     8281 tatggcggtg tttttatgcg actaaacaag gccaaatagt ccgagtctgt gaacgaattg
     8341 tcgataaaca aggcaaggtt tttaccgata cctcaagttg gtattggcag gcagaattag
     8401 gccacagcca aggtgtatgg tgggcaatta ccaccgccga agtagtggct atggtggcag
     8461 agcgataaat actgctacac ttcgcgagtt ttaacagaga ctagccgcta catgaaaatt
     8521 atccttgcac ccatggaggg
//
LOCUS       DAOQMB010000054         8501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3319_length_8501_cov_15.349751, whole genome shotgun sequence.
ACCESSION   DAOQMB010000054 DAOQMB010000000
VERSION     DAOQMB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3319_length_8501_cov_15.349751"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..1111
                     /gene="murB"
                     /locus_tag="PLY05_03385"
     CDS             83..1111
                     /gene="murB"
                     /locus_tag="PLY05_03385"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNC02377.1"
                     /translation="MVVKAMRFSENASLQTRNTFAITAKARFLADVYDEGQLAALLNT
                     VTAKHLKVLILGGGSNMLLSQDFDGLVICPQLFGVHCISEDDNQVIVEAAAGENWHQF
                     VQYCLTQGWYGLENLSLIAGSVGAAPVQNIGAYGVELKDNFVSLRAIHRQTATVREFS
                     LAECQFGYRDSVFKGRLKDQYIITAVRFRLSKIAKVSVQYGDIRQELTSKGIDTPTPQ
                     QVAQAVIAIRQRKLPNPEQIGNAGSFFKNPVISQTQFESLKQQYPHIIAYPQQQDVKL
                     AAGWLIEQAGWKGQSLGQAGVYEKQALVLVNRGSATGQEVMMLAKTIQQSVYDKFGVC
                     LEIEVNLI"
     gene            1314..2177
                     /gene="rpoH"
                     /locus_tag="PLY05_03390"
     CDS             1314..2177
                     /gene="rpoH"
                     /locus_tag="PLY05_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002115267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HNC02378.1"
                     /translation="MSNSRGLVPVTLSVPGVNLGAYISSVNQVAILTAEEERDLAERY
                     YYEQDLEAARQLVLAHLRFVVHIARSYAGYGLQQADLIQEGNVGLMKAVKRFDPSMGV
                     RLVSFAVHWIKAEIHEFVIKNWRIVKVATTKAQRKLFFNLRSLKKGSHGLTHVEAETI
                     AKDLNVTTEQVFEMEGRLSAFDASFEAHLDDDEDKQHQAPAYYLEDNRYDPARQLEES
                     DWEDNSTERLMNAMNRLDARSRDILTRRWLAEDKATLHELAAEYNVSAERIRQLEKNA
                     MDKIKAALGSV"
     gene            2250..2618
                     /locus_tag="PLY05_03395"
     CDS             2250..2618
                     /locus_tag="PLY05_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HNC02379.1"
                     /translation="MFFSLGALNLALAVILGAFGAHGLKAKVTTEQLAWWHTGVDYHF
                     YHALGLLVIGALMAAQPQLALPKGSAWALQIGIVIFSGSLYAMTLGAPRWFGAITPIG
                     GTAFIIGWLWLAYAAWQKGL"
     gene            2619..2822
                     /gene="thiS"
                     /locus_tag="PLY05_03400"
     CDS             2619..2822
                     /gene="thiS"
                     /locus_tag="PLY05_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNC02380.1"
                     /translation="MMNIQVNGEPKQVSSDCSITDLLLLLEITGRRVAVELNKEIVPK
                     SLHTTTILQENDCVEVVHAIGGG"
     gene            2844..3626
                     /locus_tag="PLY05_03405"
     CDS             2844..3626
                     /locus_tag="PLY05_03405"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HNC02381.1"
                     /translation="MNNDLLQIAGQSYQSRLLVGTGKYKDLEETRLAIEASGAEIVTV
                     AVRRTNIGQNPNEPNLLDVISPEKYTILPNTAGCYTAEDAIRTCRLARELLDGHNLVK
                     LEVLGDEKTLYPNIVETLKSAEVLVKEGFEVMVYTSDDPIVAKQLEEMGCVAVMPLGS
                     LIGSGLGIINPYNLRIIMEQANVPIIVDAGVGTASDAAIAMELGCAGVLMNTAIAKAQ
                     KPILMASAMKKAVEAGRESFLAGRMPKKLYASASSPLEGKFF"
     gene            3628..4329
                     /gene="trmB"
                     /locus_tag="PLY05_03410"
     CDS             3628..4329
                     /gene="trmB"
                     /locus_tag="PLY05_03410"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HNC02382.1"
                     /translation="MSEIVTEKQYPRRIMTFMRRSSAYTTSQQKGLDEYGEYYLLETE
                     DQRFDQQAIFGRQAPLTVEIGFGMGSSLIQMAEAAPERDFIGIEIHPNGLAQICFEAG
                     ERKLSNLRVIDGDALLLLEGYFADHSIDTVQLYFADPWPKKKHHKRRFVQTHNMQLIR
                     QKLKKGGIFHAATDWEHYAFWILEIMEASEGYRNLLGQGQFSPRPEYRPLTKFEVRGQ
                     GLGHGVWDLLYQRIV"
     gene            4414..5445
                     /locus_tag="PLY05_03415"
     CDS             4414..5445
                     /locus_tag="PLY05_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02383.1"
                     /translation="MIEVMLQRVSMFILLPMLANYSHGDLRADGHAPAGVMFEHMHHH
                     GAMIGYRVQQVTQQGSLLNGGDSLSSQSSLLTKYAMLPQKHTMTMHMLDIMYALSPKV
                     NVMIMPMYMDMGMTMTVNGLYKSTTSAHSSHGVANHSHDVRGMGDTTLAAMYQVYKDQ
                     QHELIATLAFIAPTGDESVLGGDGKPVHYGMQLGAGLWQTMPNITYNYKNHKIGFGVQ
                     LLTRQPLEDENDLGVKVPDMNQASVWLSYAWMPEFTNSIRLQHSKTDSYGGHYNVSHN
                     HSSPPDFQGNYGGEQTLIGLGANWVATVGQLKGLRVGVEYLTPINENLNGVQLETENQ
                     INFSMSKAF"
     gene            complement(5505..6701)
                     /locus_tag="PLY05_03420"
     CDS             complement(5505..6701)
                     /locus_tag="PLY05_03420"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC02384.1"
                     /translation="MSYVIQGLYDPQFEHVIDTFLAQLPQAPRQGGAALTVYHQNQKV
                     VDVWAGVANKQGDPWQEHNMSISYSTTKGVLSTLAHILVDEGLLNYDRPIADYWPSFG
                     QNGKHGITLRHVLTHEAGLYHLRSMIADAKEMLDWPHMLTMIEQAKPVHAAGARVAYH
                     AISFGWIVGGLIEKVTKEPLAVTLAKKISQPLQLDGCYIGVPESELYRCNEIIGAPRY
                     QANKAPPSKLSQWQQIISDKILRATGFEPETAAEALIPKGMSRFYLDEHTSLKACIPG
                     AGGVFTARSLAKMYAALANWGELDGVRLLSARTLAEASKVHNTQRGDVVPMAMNWRLG
                     YHRVFTTWPRTPHAFGHFGYGGSGAWADPSRRLAVGYIVNTGSGSPFADLRLWRINTA
                     IIQSVE"
     gene            complement(6714..7142)
                     /locus_tag="PLY05_03425"
     CDS             complement(6714..7142)
                     /locus_tag="PLY05_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02385.1"
                     /translation="MSVRFETIQALIVTIVNEPENTLARQTLHQAMLDANFYLAAYGI
                     PLEMKQALDQKPLIISQQLTKIPLQTATKGNSKALIAYNDLVTLQKNAPNHWQLEMTG
                     RDLLNLLLISPNFDAIVLNSAQGWVGITQEEAKALVQTTA"
     gene            7846..>8501
                     /gene="fadH"
                     /locus_tag="PLY05_03430"
     CDS             7846..>8501
                     /gene="fadH"
                     /locus_tag="PLY05_03430"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004821862.1"
                     /note="catalyzes the formation of trans-2- enoyl-CoA from
                     2,4-dienoyl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HNC02386.1"
                     /translation="MSQPFQHLLQPLDLGFVTLPNRVIMGSMHTGLEEFPPERQAAFF
                     ARRAAGGVGLIITGGISPSELGVLGPGMCAMLTEADAKKHKVITDAVHAAGGRICMQV
                     LHAGRQSYHPVNVSPSGKKSPIYPFPPQAMTEEMILQELESWVNAAALAQQAGYDGIE
                     IMGSEGYLLNQFLTVRGNERDDQWGGDFERRMRFPLEVVRRIRARVGTNFIIVYRLSM
                     L"
BASE COUNT         2499 a         1510 c         1888 g         2604 t
ORIGIN      
        1 tttgagattg tattagattt agtggaacca gccgccgaaa atctattaaa aaccctattg
       61 caaaacaaag gattaactaa ctgtggttgt taaagccatg cgttttagtg aaaacgcctc
      121 cttacaaacg cgcaatacct ttgccattac ggcaaaggca cgttttttgg ccgatgttta
      181 tgacgaaggc caattagctg ccttgttaaa tacagtgact gccaaacatc taaaagtgtt
      241 aattttaggt ggtggcagta atatgttgtt aagccaagat tttgatggct tggtcatttg
      301 cccacaattg tttggtgttc attgtattag tgaagatgac aatcaggtta ttgtcgaagc
      361 cgccgcaggt gaaaattggc atcagtttgt tcaatactgt ttaacacaag ggtggtatgg
      421 cttagaaaat ttatcgttaa ttgcaggttc ggtgggggca gcccctgtac aaaatattgg
      481 tgcttatggt gttgagctaa aagataattt tgtcagttta cgtgctattc atcgacaaac
      541 agcaacagta cgtgagttta gtttggcaga gtgccaattt ggttatcgtg atagtgtgtt
      601 taaaggccgt ttaaaagacc aatacattat tactgcggta cgttttcgct tgtcaaagat
      661 tgctaaggtg tcggtgcaat atggcgatat tcgtcaagaa ctgactagca aaggaatcga
      721 cacgccaact cctcaacaag tggcgcaagc cgtgattgcg attcgccaac gcaaactacc
      781 taacccagaa cagattggta atgcaggcag tttttttaaa aatccagtca ttagccaaac
      841 acaatttgag agtttaaaac agcaatatcc tcatattatt gcctatccac aacagcaaga
      901 cgttaaatta gccgcaggct ggttaattga acaagcaggc tggaaaggtc aaagccttgg
      961 tcaagcaggg gtttatgaaa aacaagcctt agtgttagtt aatcgtggca gtgcaactgg
     1021 gcaagaggtg atgatgttgg ctaaaactat tcaacaaagt gtttatgata aatttggcgt
     1081 gtgtttagaa atagaagtaa atttgattta aaggtgcaca gattctaatt atatcatgct
     1141 ttcgatcaaa tctttccagt gtaaagattt attacataag cgcgataaaa ctcacacact
     1201 gttgactggt caagtgtatt atttagcggt atgattaaga aataaagagg taagtataac
     1261 ttgcttcttt aacatcaaat cacatattta ttgacataat tggagacttt tccatgagca
     1321 atagtcgcgg tttagtgcct gtgacgctct ctgttccagg tgtaaatcta ggggcataca
     1381 tttctagtgt aaatcaggta gccattttaa ctgctgagga agagcgtgat cttgcggagc
     1441 gttattatta cgagcaagac ttagaagctg cccgtcaatt ggtgttagca catttgcgtt
     1501 ttgtggtaca tattgcgcgc agttatgcgg gttatgggct acaacaagct gatttaattc
     1561 aagaaggcaa tgtcggttta atgaaagccg ttaaacgttt tgacccaagc atgggtgtac
     1621 gtttagtatc gtttgctgtg cattggatta aagccgaaat tcatgaattt gtgattaaaa
     1681 actggcgtat tgttaaagtt gccactacaa aagcccaacg taagttattt tttaatttgc
     1741 gtagccttaa aaaaggtagt catggcttaa cacatgttga agccgaaacc attgccaaag
     1801 atctcaacgt gacaacagaa caagtgtttg agatggaagg gcgtttgtct gcctttgatg
     1861 cctcatttga agcacattta gacgatgatg aagataaaca gcatcaagca ccagcctact
     1921 atttagaaga caaccgttat gacccagcgc gtcaacttga agaaagtgac tgggaagata
     1981 acagtactga acgtttgatg aatgccatga atcgtttgga tgcacgttct cgtgacattt
     2041 taacccgtcg ttggttggct gaagataaag caacattgca tgaattggcc gctgaataca
     2101 acgtctctgc tgagcgtatt cgtcaattag aaaaaaatgc gatggataaa attaaagcag
     2161 ctttagggtc ggtttagatg acggactgag aaaatccctt tacacttacg ccgcgtttat
     2221 cgcggcgttt gtttttgtga gttaaaagca tgtttttttc tttaggtgcg ttaaatttgg
     2281 ctctggcggt gatcttgggt gcatttggtg cacatggttt aaaagccaaa gtcacgactg
     2341 agcaattagc atggtggcat acgggcgttg attatcattt ttatcatgct ttaggattat
     2401 tagtgattgg tgcgttaatg gctgcacagc cacaattagc cttgcctaaa ggcagtgctt
     2461 gggcgttaca aataggcatt gtcattttta gtggttcttt atatgccatg actttaggtg
     2521 ctccacgttg gtttggcgcg attacgccaa ttggtggcac agcatttatt atcggttggt
     2581 tgtggttggc ctatgctgct tggcaaaaag ggttataaat gatgaatatt caggtaaatg
     2641 gtgagccgaa acaagtctct agtgattgtt caatcaccga tttattgttg ttgttagaga
     2701 taacaggtcg tcgggtggcg gttgaattaa acaaagaaat tgtgcctaaa agtttgcata
     2761 caacaaccat attgcaagaa aatgattgtg tagaagtggt tcatgccatt ggtggcggtt
     2821 gacatttaaa agagttgtga atgatgaata atgatctatt acaaatagcg ggacagagtt
     2881 atcagtcgcg tttattggtt ggtacaggta aatataaaga cctcgaagaa acacgcttag
     2941 cgattgaggc aagcggtgca gaaatcgtga ctgtcgccgt acgtcgtacc aatattggcc
     3001 aaaatccgaa tgaaccaaat ttactagacg tgatttctcc cgaaaaatac accattttac
     3061 caaacacagc aggttgttat accgcagaag atgcgattcg tacctgtcgt ttagcgcgtg
     3121 agttattaga tggtcataac ttagtaaagt tagaagtgtt gggtgacgaa aaaaccttat
     3181 atcccaatat tgttgaaacc ttaaaatcgg ccgaagtatt ggttaaagaa ggttttgagg
     3241 tcatggttta tacctctgat gatccgattg tcgctaaaca acttgaagag atgggctgtg
     3301 tggccgtaat gcctttaggt agtttaattg gctcaggttt agggattatt aacccctata
     3361 acttgcgtat tattatggaa caagccaatg tgccaattat tgtcgatgca ggtgtgggta
     3421 cagcctcgga tgctgcgatt gcgatggagt taggttgtgc aggtgtatta atgaataccg
     3481 cgattgccaa agcacaaaaa cctattttaa tggcgtcagc aatgaaaaaa gcggttgaag
     3541 ctggccgtga atcctttttg gcaggtcgta tgcccaaaaa attatacgct tctgcttcca
     3601 gtcctttaga aggtaagttt ttttaacatg tctgagatcg ttactgaaaa acaatatcca
     3661 cgtcgtatta tgacttttat gcgtcgttcg tcggcttata ccacgtcaca gcaaaaaggt
     3721 ttagatgaat atggcgagta ttatttgtta gagactgaag atcaacgttt tgatcaacaa
     3781 gctatttttg gtcgtcaagc accattaacg gtagaaattg gctttggtat gggcagttca
     3841 ttaattcaaa tggctgaggc tgctccagag cgcgatttta ttggcattga aattcatccc
     3901 aatggtttgg cgcaaatttg ttttgaggca ggtgagcgta aattaagcaa tttgcgggtg
     3961 attgatggtg atgccttatt attgttagag ggctattttg ccgatcatag tattgatacc
     4021 gtgcaattat attttgctga tccgtggcca aaaaagaaac accataaacg ccgttttgtg
     4081 caaacccata atatgcaatt gattcgtcaa aagttaaaaa agggcggcat cttccatgcg
     4141 gcgactgatt gggagcatta tgcgttttgg attttagaaa ttatggaagc cagcgaaggc
     4201 taccgtaatc tattgggtca aggtcagttt tcaccacgtc ccgaatatcg tcctttaacg
     4261 aagtttgaag tccgtggcca aggattaggg catggcgtat gggatttgtt gtatcaaagg
     4321 attgtttagc tttggttgct ttgccctgcg acaatatgtc gcatcggaaa aagtagcttt
     4381 ttttattact atacacacgt tttgaacatt tgagtgattg aagtcatgtt acaacgtgtg
     4441 tcgatgttta ttttattacc aatgttggcg aattatagtc atggtgattt gcgtgcagat
     4501 ggccatgcac cagcaggtgt tatgtttgag catatgcatc atcacggtgc catgattgga
     4561 tacagagtgc aacaggtgac tcagcaagga agcttattaa atggtggaga ctcattgtca
     4621 tcgcaaagtt cattattaac taaatatgcg atgttgcctc aaaaacatac tatgacgatg
     4681 catatgttgg acattatgta tgcgttatca cctaaagtta atgtaatgat tatgcctatg
     4741 tatatggata tgggcatgac gatgacagtt aatggattat ataaatcaac aacaagtgcg
     4801 catagttctc atggtgttgc aaatcacagt catgatgttc gagggatggg tgatacaaca
     4861 ttggcagcga tgtatcaagt ctataaagat caacaacatg agttaattgc aacattagct
     4921 tttattgctc ccacaggaga tgagtctgta ttgggaggcg atggcaaacc tgttcactac
     4981 ggaatgcaat taggggcagg cttatggcaa accatgccca atattaccta taactataaa
     5041 aatcataaaa ttggctttgg agttcagtta ttaacacgtc aacctttaga agatgagaat
     5101 gatctaggtg ttaaagtacc tgatatgaat caagcaagtg tttggttaag ctacgcttgg
     5161 atgccagaat ttactaattc tatccgtttg cagcatagca aaacagatag ttatggtggc
     5221 cattataatg tttcccataa tcatagttct ccacccgatt ttcaaggtaa ttatggtggt
     5281 gaacaaacgt taatcgggct tggtgctaac tgggttgcaa ccgtaggaca actaaaggga
     5341 ttaagagtag gtgtagaata tttgacaccc attaatgaaa atttgaatgg tgtgcaatta
     5401 gagactgaaa accaaatcaa tttttctatg agtaaagctt tctagaaaaa tctaagacct
     5461 tgagggtttt agttgaaaca tctcaaggtc ttaataccca aaaattattc aacactttga
     5521 ataatcgccg tattaattcg ccatagtcgt aaatcggcaa atggcgaacc actccccgta
     5581 ttaacaatat atccaaccgc taatcgtcta gatgggtcag cccatgcacc agagccacca
     5641 taaccaaaat gtccaaaagc atgaggagta cgaggccatg tggtaaacac acggtgataa
     5701 cctaaacgcc aattcattgc cataggcaca acatcacctc gctgggtatt atgcactttg
     5761 cttgcttcgg ccaatgtacg tgcagaaagt aatcttacgc catcaagctc tccccaatta
     5821 gcaagagcag catacatttt agctaatgaa cgtgcagtaa atacaccacc agcaccaggg
     5881 atacaggctt ttaaactagt atgttcatct aaataaaatc ggctcatgcc tttaggaatc
     5941 agtgcttctg ccgcagtttc tggttcaaag cctgtcgctc ttaaaatttt atcactaata
     6001 atctgttgcc attgacttag tttgctagga ggagctttat tagcttgata acggggcgca
     6061 ccaataattt cgttacaacg atataactct gattctggaa caccaatata acaaccatct
     6121 aactgtaacg gttggctgat ttttttggct aatgtgacag ctaaaggctc tttggtgact
     6181 ttttcaatca gaccaccgac aatccaacca aaagaaatgg catgataggc aactcttgca
     6241 ccagccgcat gaacaggttt ggcttgttca atcatggtta acatatgcgg ccaatcaagc
     6301 atttctttgg catcagcaat catgctacgc aaatgatata aaccagcttc atgggttaaa
     6361 acatgacgta aagtaatgcc atgcttacca ttttggccaa aagaaggcca atagtcagca
     6421 attgggcgat cataatttag caatccttca tcaactaaaa tatgagcgag agtactcaaa
     6481 acgccttttg ttgttgaata actaatgctc atattatgct cttgccacgg atctccttgt
     6541 ttattggcaa cgccagccca tacatccacc actttttgat tttgatgata taccgtcagt
     6601 gctgcaccac cttggcgcgg tgcttgtggc aactgcgcca aaaaagtgtc tataacatgt
     6661 tcaaattgtg gatcgtatag tccttgaatg acataactca taacaagaag tctctatgct
     6721 gtggtttgca caagtgcttt tgcttcttct tgtgtaatgc ccacccagcc ttgtgctgaa
     6781 tttaagacaa tagcgtcaaa attagggcta attagcagca aatttaataa atcacgtcct
     6841 gtcatttcta gttgccagtg attaggcgca tttttttgta aggttactaa atcgttataa
     6901 gcaattaatg ctttgctgtt gccttttgtg gcagtttgta atggaatttt ggtcaattgt
     6961 tgagaaataa tcagaggctt ttgatctaaa gcttgtttca tttctaaagg aataccataa
     7021 gcggccaaat aaaaattagc atcaagcatc gcttgatgaa gtgtttgtcg agctagtgtg
     7081 ttttcaggct cattaactat agtaacgatg agtgcttgga tagtttcaaa tctgacggac
     7141 ataatagtaa accaataatt ttttagacga agagaaggat gactttataa atggaattgt
     7201 tttgatattc actgagttct cacaaaaaaa caatccttta cacacttatt tttatatatg
     7261 ataacgataa tcatttttat ttggttgttg ttaggggcaa ttgtcattaa attgtcttaa
     7321 ctttgtcatg gtacggtaat tgcttataga tttaataatg gtattgaagc tataaatgta
     7381 acaatagcgt caacagaccc taatctaatt tggagtgaat catggaatta ttagaaatac
     7441 gcgatgagag tttaattgat gagtgggctg attatgaaga ttatgactat gttgatgatg
     7501 caacagagtt ataactttta gttataaaaa atattttttt atggcgaaca cggagtcgtt
     7561 aaaacgactt gtctgcacgt ggtaggcaga gtattgttaa acacaagaaa aagccgtttt
     7621 attaaaaagt ttgtacttag tcatgataat gtaaaactaa gtgtaaatgt tacttggtac
     7681 tttcgtgcta ataactctct tttttgcttg acgagagcta tctccttgtg acataagaaa
     7741 cggctaattt tttagaactg ttgtacgcat gctataaaac agcataaata ttggagttgt
     7801 acagcaattt aaacctacca agtgctagct agctgagggt tcgccatgtc acagccattt
     7861 caacatttat tacaaccatt agatttaggt tttgttacct tgccaaaccg tgtcatcatg
     7921 ggttcgatgc acacaggtct tgaagagttt ccgcccgaaa gacaagcggc tttttttgca
     7981 cgtcgtgccg caggcggtgt gggcttgatt attactggtg gtatttcgcc atctgaatta
     8041 ggcgtgttag ggcctggtat gtgtgccatg ctcaccgaag ctgatgctaa aaagcataaa
     8101 gtgattaccg atgcagttca cgcggcaggt ggtcgtattt gtatgcaggt attacacgca
     8161 ggtcgtcaat catatcatcc tgttaatgtt tctccctctg gtaaaaagtc accgatttat
     8221 cctttcccac ctcaagcgat gactgaagaa atgattcttc aagagttaga gagttgggtc
     8281 aatgcggctg ctttagcaca acaagcgggc tatgatggca ttgaaatcat gggttcagaa
     8341 ggctatttgt taaatcagtt tttaaccgtt cgtggtaatg agcgtgatga tcaatggggt
     8401 ggtgattttg aacgccgtat gcgtttccca ctagaagttg ttcgtcgtat tcgtgcgcgt
     8461 gttggcacca actttattat tgtctatcgt ttgtcgatgc t
//
LOCUS       DAOQMB010000055         8455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3348_length_8455_cov_17.111667, whole genome shotgun sequence.
ACCESSION   DAOQMB010000055 DAOQMB010000000
VERSION     DAOQMB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_8455_cov_17.111667"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..606)
                     /locus_tag="PLY05_03435"
     CDS             complement(10..606)
                     /locus_tag="PLY05_03435"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HNC02387.1"
                     /translation="MAITLPALPYANDALAPHITPETIEFHHGKHHNAYVVNLNNLIK
                     DTDLADKDLEAIIQAVAGDASKAGVFNNAAQVWNHTFYWNSMKPNGGGKPTGAIAAKI
                     DADFGSYEKFVELFTAAATTQFGSGWAWLVVGADGKLAITKTGNADTPIVHGQKPLLT
                     IDVWEHAYYIDFRNLRPKYIATFLESLVNWDFANANLA"
     gene            complement(672..1274)
                     /locus_tag="PLY05_03440"
     CDS             complement(672..1274)
                     /locus_tag="PLY05_03440"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF011576.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin C"
                     /protein_id="HNC02388.1"
                     /translation="MAVLVGKPAPDFTVPAVLANGEIVDSYNFAEATKGKYALVFFYP
                     LDFTFVCPSELIALDHRIPDFQARNVEVVGVSIDSHFTHNAWRNTPVNAGGIGPVKYT
                     LAADITHEIVKAYDVEAGGGVAYRGAFLIDKNGIVRSQIINDLPLGRNMEELLRLVDA
                     LQFTEEHGEVCPANWKKGDKGMTATPDGVASYLAENADKL"
     gene            complement(1453..2160)
                     /locus_tag="PLY05_03445"
     CDS             complement(1453..2160)
                     /locus_tag="PLY05_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HNC02389.1"
                     /translation="MKKKTHWRLKVLKWHRRIGVVLSVFLLWMLASGVLINHANDLQL
                     DKQQLNNEFWLKWYGLSSTNTQLIGDKSLSLNKQGLWLNQQNLGECSSLLGVVKSSQE
                     VVVVCPEHILLLLPTGELIDHVDKSRGLSIVSQKMAVVEQKIIVLANNDQLYQLDTQS
                     LSFSPFLPVTTPINWQEIQQPQAQLSYEQWLLDAHSGRLFGAWGKWLVDVLSVLLIML
                     IVSGWFLAKRRHQLKNV"
     gene            complement(2157..2660)
                     /locus_tag="PLY05_03450"
     CDS             complement(2157..2660)
                     /locus_tag="PLY05_03450"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HNC02390.1"
                     /translation="MWRIVAFIAGLTVQSMAHAALSNEEFIQKYLKQPVENKVLWLNK
                     PLQQRAATILGHNYQGLRVRYWQSGNRSAWILDEIGKEQPITTGVVIENNQIVAVDVL
                     VYRESRGGEVQQNFFTKQFQGLTLDKNDKLSKKIDGITGATLSVWALQKVARLALMFD
                     SQKGATP"
     gene            complement(2768..3940)
                     /locus_tag="PLY05_03455"
     CDS             complement(2768..3940)
                     /locus_tag="PLY05_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02391.1"
                     /translation="MLKLSVATLVLSAAISTVYANDDLAQLKAQLKAQQAQIDALVAA
                     SEKQTSAAGSAFANTTLGGYGEAYFKHIDGEKDEFDAYRLVLFVGHKFNDKVRFSSEL
                     EIEHAYVKDTDTGTASPSKTEGYVALEQLFLEYQYLPKHRVAAGQLLVPVGILNETHE
                     PDTFYGTFRAPVEREIIPSTWFETGLMASGEIVSGLSYDAMLSSGLKNDGSKAIKEGR
                     QRGSKADGSDLAYTARLKYTGIAGFEWGATWHHQTDMSQGSPKVANQNLDADLLETHI
                     AVQKNIFGLRALYAKWDVNKSALAIADTKYAEQEGWYVEPSVKVLPTVGIFARYSEWD
                     NMAADSVSSNWQEQSLGISWWLDERAVVKADLQRRDDPNGNNTDKNGFNLGIGFSF"
     gene            4249..6444
                     /locus_tag="PLY05_03460"
     CDS             4249..6444
                     /locus_tag="PLY05_03460"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNC02392.1"
                     /translation="MSSLENKFPFSQLRLVAAIGLFAASQSLFAAEIAGRVNFVSGSA
                     FVSNAQNQQRNVFKGDLIYAGEKIETGRNSRIQIKMTDGGTIVLRPSSTFEITQYSFS
                     KDTPELGTVLFNFIKGGARAISGAIGKVNKQNYKFNTPVATIGIRGTDYSASLDDNKL
                     LVTVSDGKISITNQQGEEEAKEGETFAVNADQKPTLCHTVNTAGGTCDPVFISLDTDD
                     NLSFSRQKVKKPTIENFATYGAFTEAMRRYKSLEQEVQRIEDDFSVNDVIEKATKPVD
                     TPRLTPFAQTDLAGFDNLDNAELSNFSVENAIGIIEDQLDTDINQGMINDINNEDILL
                     KKVFKYKLEEDTTEITSKVAYNLADFIDFKGFIENLFLLNTDTLYGNNMRMQGVEVGN
                     PKINVELGNSIFDAINVQLAAATRNKSYALNLGLNDNNLFSLLDDNKNNQFGGLLSIR
                     QVAIYDRDGLGVKDSIGGFLQIGNGSPSDAILVLTDDNTPIKVSLDLFEIQRLPSSKG
                     FFDLANKTTINVNLQTPKLLVKLGSVYIGGFNTITDQDTDRVVSHEEVTDSSFNRQSL
                     TKILEASQIVLGEATISARLEHTPLRKTNRIDDISVNPNISIFADAFVKSGVTIYNFD
                     LLDTGGSIRGGGLGFSKMSISDYASQNLTAKLAVYIETDLKDNNKLKLFGGNVDGLVL
                     VLDQLGTVNDGVDLNISNFRVGTQTQKNIGDVQIKGLNINGTKVILRGH"
     gene            6456..6845
                     /locus_tag="PLY05_03465"
     CDS             6456..6845
                     /locus_tag="PLY05_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02393.1"
                     /translation="MRALLMYSTKLYFLLSIWSVSFVANAEDANSIVPSSVFNMASSA
                     KTDAVKKDIDENKPRLENYASYNDFLNAMYVYNKTQEDNIKPRIIIDLPTKPDHQPEY
                     TFLEKNADVTGKGETIPDVVPEQQNLK"
     gene            6814..8013
                     /locus_tag="PLY05_03470"
     CDS             6814..8013
                     /locus_tag="PLY05_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02394.1"
                     /translation="MLFLSNKISNNMSRIFFLLCILTCHAYAAEQIGKVSNIKGDVFV
                     IDANNSKRNLFKGDFLYTQDKIQTDVNSLVSLETINAHSFTLFQNTTLILSDYNYNKN
                     SPELNTLNFDLISGTIKVNNSAASIKNTNLIQTPYLNVESTLADYLLQFTPPNMSIIT
                     INQGSARLLTKNLVTTITQGQTFEVKEDNIPLLSSIIIPQIPSLDRLEKKTHISPNNI
                     SAKEGRPRLENYSNYKDFIQAMYLFKKAEEELKRPKIIIPNLPSGENLPEYSLIDDGY
                     EVTNWAEDLTNLNKDYNIDNIQLVKQSSKMAIMTKDEMELSAVKDIFGVTFFSLADFL
                     NMKVEELNRLISTKNTDDGAVEQQKSNDLMSKLLKDSIKTNAIGNDISIIGVELGQPE
                     ITVIKRP"
     gene            complement(8047..8373)
                     /gene="grxD"
                     /locus_tag="PLY05_03475"
     CDS             complement(8047..8373)
                     /gene="grxD"
                     /locus_tag="PLY05_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007393725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HNC02395.1"
                     /translation="MMDIESLIKKQISENAVILYMKGTPQFPQCGFSARTVEALMQIG
                     EPFAYVNILENPDIRATLPKIANWPTFPQLWIKGELIGGCDIVLEMTQKGELAPLVKE
                     AAQKAE"
BASE COUNT         2766 a         1542 c         1438 g         2709 t
ORIGIN      
        1 ggttggctat taagctaagt tagcgttagc aaaatcccag ttcactaagc tttctaagaa
       61 agtagcgatg tattttggac gcaaattacg gaaatcgatg taataagcgt gttcccatac
      121 gtcaatcgtt aataatggct tttgaccgtg aacgattggg gtatcagcat tgccagtttt
      181 ggtgattgct aatttgccat cagcaccaac cactaaccat gcccaaccag aaccaaattg
      241 tgtagttgca gcagcagtaa ataactcaac aaacttttcg tagctaccga agtctgcatc
      301 gatttttgca gcaatcgcgc ctgttggctt gccaccaccg tttggcttca tggagttcca
      361 gtaaaaagtg tggttccaaa cttgtgcggc attgttaaat acgccagctt tgcttgcatc
      421 accagcaacg gcttggataa tcgcttctaa atctttatca gctaaatcag tatctttgat
      481 taagttgttt aagttaacaa cataagcatt gtggtgttta ccgtggtgaa actcgatggt
      541 ttctggggta atgtgtggtg caagtgcgtc gtttgcgtat ggtaaagctg gcaatgtaat
      601 ggccatgagg ctagctccta aataaatgtg atttattaag ccattgtaaa acaatggctt
      661 aaattaataa attagagttt atcagcgttt tcggctaaat aagaagcaac accatcaggc
      721 gttgcagtca tacctttgtc accttttttc cagtttgctg ggcaaacttc accgtgttct
      781 tcagtgaatt gtaaggcatc aactaaacgt aataactctt ccatattacg gcctaatggt
      841 aaatcgttaa taatttgaga acgaacgata ccgtttttgt cgattaagaa agcaccacgg
      901 taagcaacac caccgcctgc ttctacatca taagccttaa caatttcatg agtaatatca
      961 gcagctaaag tatatttaac tgggccaata ccacctgcgt ttacaggtgt gttacgccat
     1021 gcattgtggg taaagtgaga gtcaatagac acaccaacca cttctacatt acgtgcttgg
     1081 aaatctggaa tacggtgatc taaagcgatc aattcagatg gacaaacaaa ggtgaagtct
     1141 aaaggataga aaaatactaa tgcgtattta cctttagtcg cttcagcaaa gttatagctg
     1201 tcaacgattt cgccattggc taaaacggct ggtacggtaa aatctggggc tggcttacct
     1261 actaatacgg ccatgataaa ctcctttgag ttaaatatgt gttgttatga cagtctaaag
     1321 acctagaaat ttactaggat tttagctacc gcgaaagatg aggctttatt tatcatattt
     1381 caagccaaca aagcacagca ttcgtatttt gtcacaactt tttagatgac ttgcacaaaa
     1441 aacagtctta atttagacat tttttaattg gtggcggcgt ttggccaaaa accagccact
     1501 cacaatcaac ataatcaaca agacactcaa aacatctact agccattttc cccacgctcc
     1561 aaataaacgt cctgagtgtg catctaataa ccattgctca tagcttaatt gtgcttgtgg
     1621 ctgttgaatt tcttgccaat tgataggcgt tgtgacaggc aaaaaaggac taaaacttaa
     1681 agattgggta tctaattgat agagttggtc attattggct agaacaatta ttttttgttc
     1741 taccacagcc attttttggg aaacgataga taaaccacgg cttttatcga catgatcaat
     1801 taattcgcct gttggtaaca ataaaagaat atgttctgga cacaccacca ctacttcttg
     1861 agatgacttt actacaccca ataggctaga acactcaccc aagttttgtt gattaagcca
     1921 taagccttgt ttatttagac ttaagctttt atcgccaatc aattgtgtat ttgtgcttga
     1981 taaaccatac cattttagcc aaaactcatt gttaagctgt tgtttatcaa gctgtaaatc
     2041 attggcatga ttaattaata caccactagc caacatccac aataaaaata cactcaaaac
     2101 tacaccaatc cgtcgatgcc attttagaac ttttaaacgc caatgcgttt ttttcttcat
     2161 ggtgtagcac ctttttggct atcaaacatt aaggctaaac gcgcaacttt ttgtaatgcc
     2221 caaaccgata aagttgcgcc tgtaattcca tctatttttt tgctgagctt gtcattttta
     2281 tcgagggtta atccttgaaa ttgcttcgta aaaaagtttt gttgaacttc cccaccgcga
     2341 ctttcacgat aaaccaaaac atctacagca acaatctgat tattttcaat tacaacacca
     2401 gtggtaatgg gttgctcttt accaatttca tccaaaatcc aagcactacg attgcctgac
     2461 tgccaataac gtactcttaa tccttgataa ttatgcccca aaatggtggc ggcacgttgt
     2521 tgtaatggct tgtttaacca taacacctta ttttcaactg gttgctttaa atatttttga
     2581 ataaattctt cattgcttaa cgccgcgtgc gccattgatt gcactgttaa accagcaata
     2641 aaagctacta ttcgccacat aaacaaccta tgtttaacca ttaattgtta atcataacaa
     2701 aaaaaggcct atatcgcttt gatatacgcc ttttttcatc tttagaatga cattttggct
     2761 ttcatgttta gaatgaaaaa ccaataccta gattaaatcc atttttatca gtattgttgc
     2821 cattaggatc atcacgacgc tgtaaatccg cttttactac ggcgcgctca tccaaccacc
     2881 aactaatacc taatgattgt tcttgccaat tagaacttac actgtcggca gccatattat
     2941 cccactcgct gtaacgagca aaaataccta cagtcggcaa tacttttacc gaaggctcaa
     3001 cataccagcc ttcttgctca gcatatttag tgtccgcgat tgctaaagct gatttattga
     3061 catcccattt ggcatacaaa gcgcgtaaac caaaaatatt tttttgcaca gcaatatgtg
     3121 tttctaataa atcagcatct aaattttgat tggcaacttt tggcgagcct tgagacatat
     3181 ctgtttgatg atgccatgtt gctccccatt caaaaccagc aatacctgta tatttgagac
     3241 gtgcggtata agctaaatcg ctaccatcag ctttagaacc acgttggcga ccttctttaa
     3301 tggcttttga gccatcattt tttaaaccac tagataacat ggcatcataa cttaagcccg
     3361 atacaatctc accagaagcc attaaacctg tttcaaacca tgtactaggg ataatttcgc
     3421 gttctactgg cgcacgaaac gtaccataaa atgtatcagg ttcatgggtt tcatttaaaa
     3481 taccaacggg cactaatagt tgtcctgctg caacacgatg tttaggtaaa tattgatact
     3541 ctaaaaataa ttgttctaaa gcaacataac cttcggtttt gcttggtgat gctgtacctg
     3601 tgtctgtatc ttttacataa gcatgttcaa tttctaattc actagaaaat ctgactttat
     3661 cattaaattt atgacctaca aacaatacca aacgataggc atcaaactca tctttttcac
     3721 catcaatgtg cttaaaatat gcttcgccat aaccccccaa agttgtattt gcaaatgcag
     3781 agccagcagc tgatgtttgt ttttcggaag cggcaaccaa ggcatcaatt tgtgcttgtt
     3841 gggcttttaa ttgcgctttt aattgggcta aatcgtcatt agcataaaca gtagagatgg
     3901 ctgcagaaag aactaatgtg gctacactca gctttaacat agataactac cttagatgtg
     3961 acaaaatgtt tcatacaatt ttatcaaaaa caaaactaaa tgcaaattat tatcatttaa
     4021 ttactcacta attatgaaaa ttgaatcttt ctatgtgatg ttatgcacta aaaaaattgt
     4081 tttttgttac tttatcagtt gcaaaatgtt taccttttgt tgataaggta ttagactgtg
     4141 gagtggaaag acaaatttct taacttatac aataagaatc ataactaata attttttata
     4201 aatgacagac aatttgctgt ggacaccctg accacagaag gacttcttat gagctcattg
     4261 gaaaataagt ttcctttttc tcagcttcga ctagttgctg ctattggatt atttgcggca
     4321 tctcagtcat tgtttgctgc cgaaattgct ggtcgggtca attttgtttc tggcagtgcg
     4381 ttcgtcagta atgcccaaaa tcaacagcgt aatgttttta aaggtgatct gatttacgca
     4441 ggcgaaaaaa tagaaacagg tagaaatagc cgcatccaaa ttaaaatgac tgatggcggt
     4501 acaattgttt tacgtccaag ctcaacattt gaaataaccc aatacagttt tagtaaagac
     4561 acgcctgaat taggcacagt cttatttaat tttattaaag gtggcgcacg cgcgattagc
     4621 ggagctattg gtaaagttaa taaacaaaac tataaattta atacacctgt tgccactatt
     4681 ggtattagag ggacagatta ttctgcttca ttagatgata ataaactact tgtgactgtc
     4741 agtgatggaa aaattagcat tactaatcaa caaggtgaag aagaggcaaa agaaggtgaa
     4801 acttttgctg tgaatgccga ccaaaagccg acgttatgtc atactgtgaa tacagctggc
     4861 ggtacttgtg accctgtttt tatttcttta gatacagatg acaatttgtc tttttccaga
     4921 caaaaagtca aaaaaccaac aattgaaaat tttgctacct atggtgcttt taccgaggca
     4981 atgcgccgct ataagtcttt agaacaagaa gtgcaacgta ttgaagatga ttttagtgtt
     5041 aatgacgtta tagagaaagc cacgaaacct gttgatactc cccgtctcac tccatttgcc
     5101 cagactgatt tggcaggatt tgataatctt gataatgccg aactaagcaa tttttctgtt
     5161 gaaaatgcta ttggtattat tgaagatcaa ttagatacgg atatcaatca gggcatgatt
     5221 aatgatatta ataatgaaga tattctttta aagaaagttt tcaagtataa actcgaagaa
     5281 gatactaccg aaatcacatc aaaagttgct tataatttag cggactttat agattttaaa
     5341 ggtttcattg aaaatctatt tttgttaaat actgatacac tttatggtaa caatatgcgt
     5401 atgcaaggtg ttgaggtagg taatcccaaa attaacgtag aattaggtaa tagtattttt
     5461 gatgctatta atgtgcagtt agccgcagca acacgtaaca aaagttatgc gttaaattta
     5521 ggcttaaatg ataataattt attttcatta ttagatgata ataaaaacaa tcaatttggt
     5581 ggtttattaa gtattcgtca ggtagctatt tacgatagag atgggcttgg agttaaagac
     5641 tcaattggcg gttttttaca aattggaaat ggttctccta gtgatgctat tttggtctta
     5701 acagacgata acactcctat taaggtatct ttagatctat ttgagattca aagactcccc
     5761 tcttctaaag gattttttga tttagccaat aaaacaacta ttaacgttaa tttacaaacc
     5821 cccaagttgc ttgttaaatt aggcagcgtt tatattggag gttttaatac tattaccgat
     5881 caagatactg atagagttgt aagccacgaa gaagtcactg atagtagctt taatcgtcaa
     5941 tctctgacaa aaattctgga agcatcacaa atagttctcg gtgaagcaac tattagtgct
     6001 agattagaac acactccact tcgcaaaact aatcgtatag atgatattag tgtcaatcct
     6061 aatatttcta tctttgcaga tgcctttgtt aagtcaggcg ttacaattta taattttgat
     6121 cttcttgaca ctggaggatc aattcgtggc ggaggcttag gatttagcaa aatgagcata
     6181 tctgattatg caagtcaaaa tttaactgcc aaattagctg tatatataga aactgactta
     6241 aaagacaata ataagctcaa attatttggt ggtaatgtcg atggtttggt tcttgttttg
     6301 gatcaacttg gtacagtcaa tgatggtgtt gatttaaata tttcaaattt tcgagttggg
     6361 actcaaactc aaaaaaatat aggcgatgta cagattaaag gcctaaatat caacggtaca
     6421 aaagttattt tacgtggcca ctaagagtta atttaatgcg agctttatta atgtatagca
     6481 caaaattata ttttttatta tctatttggt ctgtatcttt tgtcgctaat gctgaggatg
     6541 caaatagtat tgtacctagc agtgttttta atatggcatc atccgccaaa actgatgcag
     6601 ttaaaaaaga tattgatgaa aacaaaccgc gtttagaaaa ttatgctagt tataatgact
     6661 ttctcaatgc gatgtatgta tataataaga ctcaagaaga taatataaaa cctcgcatta
     6721 tcattgacct acctacaaaa ccagaccacc aaccagaata tacattttta gaaaaaaatg
     6781 ctgatgttac tggtaaagga gaaactatcc ccgatgttgt tcctgagcaa caaaatctca
     6841 aataatatgt ctcgaatatt ctttttatta tgcatattaa cttgtcatgc ctatgcagca
     6901 gagcaaattg gcaaagtaag taatattaaa ggtgatgttt ttgttattga tgctaataat
     6961 agcaaacgta acttatttaa aggtgatttt ttatataccc aagataaaat tcaaactgac
     7021 gtaaatagcc ttgtatcatt agaaactatt aatgcgcact ccttcaccct atttcaaaat
     7081 actactctta ttttaagcga ctataactat aataaaaatt ctccagaatt aaatacgctt
     7141 aattttgatt taatttcagg cacaatcaaa gtaaataatt cagcagcatc tataaaaaat
     7201 acaaacttga ttcaaacgcc ttatttaaat gttgaatcta ctttggcaga ttatttatta
     7261 caatttactc ctccaaatat gtcgataatc actattaatc aaggttctgc cagattatta
     7321 accaaaaact tagtgactac gattactcaa ggtcaaactt ttgaagttaa agaagataat
     7381 ataccactcc tatcatcaat aattattcct caaataccct cattagatag attagaaaaa
     7441 aagactcata tatctcccaa caatatttca gctaaagagg gtcgaccacg tttagaaaat
     7501 tactctaact ataaagattt tattcaagct atgtatttat tcaaaaaagc cgaggaagaa
     7561 ttaaaacgac ctaaaattat tattcctaac ctaccatctg gtgaaaactt accagaatac
     7621 tcattaattg atgatgggta tgaggtaact aattgggcag aagatttaac taatttaaat
     7681 aaggattata atattgataa tatacaatta gttaaacagt cttcaaaaat ggcgataatg
     7741 actaaagatg aaatggagtt gtcagctgtt aaagatattt ttggtgtcac atttttttct
     7801 ttagccgatt ttttaaacat gaaagttgaa gaattaaacc gtctaatttc aacaaaaaat
     7861 accgatgatg gtgcagttga gcaacaaaaa tctaatgatc taatgagtaa acttctaaaa
     7921 gatagtataa aaaccaatgc aattggcaat gacatttcta tcattggtgt tgaattagga
     7981 cagcctgaaa ttaccgttat taaacgccca taataaaaag gcttgtaaat acaagccttt
     8041 tttagtttat tctgcttttt gtgcagcttc tttcactaat ggtgctaact cgcctttttg
     8101 agtcatttct aagacgatat cacaaccacc aattaactca cctttaatcc ataattgtgg
     8161 aaaagttggc cagttggcaa tttttggtaa ggttgcacga atatcaggat tttctaaaat
     8221 attaacataa gcaaatggtt caccaatttg cattaatgct tcaacagtac gcgcagaaaa
     8281 gccacattgg ggaaattgtg gtgtgccttt catgtataaa ataacagcat tttcgctaat
     8341 ttgctttttg attaaggatt caatatccat catacaccta cgataaccta gttttagact
     8401 cggatattgt aacgctttta acaacaaatc gtcagtgtaa aactaaattt tgtcg
//
LOCUS       DAOQMB010000056         8432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3360_length_8432_cov_15.270741, whole genome shotgun sequence.
ACCESSION   DAOQMB010000056 DAOQMB010000000
VERSION     DAOQMB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3360_length_8432_cov_15.270741"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1338)
                     /gene="serB"
                     /locus_tag="PLY05_03480"
     CDS             complement(112..1338)
                     /gene="serB"
                     /locus_tag="PLY05_03480"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HNC02396.1"
                     /translation="MREIILMSFSGPDRPRLSASLMEVLAQHAVNILDVGQAVIHDQL
                     SLGVVVESPSAQDSALLMKDVLFRAHDIGLLVRFTPITLDSYHQWVSGQGKARYIVTL
                     LARKITAEQLAAVTQIVADNGLNIDGLNRLSGRVPLEDENAGLGKACVEFSVRGEPNN
                     INSMRAEFLRLANDLNMDIAFQRDDAYRRNRRVVCFDMDSTLIEHEVIDELAKAAGVG
                     EQVAAITERAMQGELDFKQSFEARVALLKGLNASVLAEIAARLKLSEGAENLVKTLKA
                     LGYKTAILSGGFTYFGQYLQHRLGIDYVFANDLEIVDGQVTGRVVGQVVDGQRKAELL
                     RDIAAKEGVRLEQVIAVGDGANDLPMLSIAGLGIAFRAKPLVRQNARQAISTLGLDGI
                     LYLLGVRDRDLDLGKS"
     gene            1499..2650
                     /locus_tag="PLY05_03485"
     CDS             1499..2650
                     /locus_tag="PLY05_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02397.1"
                     /translation="MSQVRGIVPLFVASYFLHAMLLVNSAETELANHQKPIGQLLSEQ
                     LAISAAPLIANQDSVGLGLLANNVGSTEAILGLKISNSQQQVLAAGGQGQSQQGQNFI
                     APIQLNNKVLGSVEITLSSAQRGEIIQHSVVNLVLSFVVHLFLALWIGWPQLFSNIRI
                     PILQEPKAPTPEPEPIVPIVEPEPIKAAASVWVAIGFDDPKGLISKVNANTINQFLQV
                     VDKLVKRTTRLYSGKMVTQLNSNGVVIRFDGDHVSDCAHRALLSSRLLIKLVSTAYGQ
                     RREAKQFALKVKASLLNLAELTDNDADKKLKAVLNVSKVNQVLVVDDELLLNDLKAKH
                     QLVAFEASEEASKAEKELKAHIVEALATAQEEELSGLEKRILERKKPNA"
     gene            2660..3019
                     /locus_tag="PLY05_03490"
     CDS             2660..3019
                     /locus_tag="PLY05_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC02398.1"
                     /translation="MSDFIAIETLINDYFQALHSKDVAKIETIFWDHASITGYYEGEF
                     VHSKLHDYLSILKRMSAPNMIGEDFDMEVVSIELKGNMAAVKTRYLFQALEYVDFLSL
                     LKIDDNWQIIQKTFYHD"
     gene            complement(3057..5282)
                     /gene="parC"
                     /locus_tag="PLY05_03495"
     CDS             complement(3057..5282)
                     /gene="parC"
                     /locus_tag="PLY05_03495"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HNC02399.1"
                     /translation="MSALVTDFTESRSLAEFTEQAYLNYSMYVILDRALPHIGDGLKP
                     VQRRIVYAMSELGLKNSAKHKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYP
                     LVDGQGNWGSPDDPKSFAAMRYTESKLSAYADVLLAELGQGTVDWVPNFDGTMEEPSL
                     LPARLPNLLLNGTTGIAVGMATDIPPHNLREVASACIALLKKPNLSLEEIAAYIPAPD
                     FPTKAEIITAKSELLNLYKTGRGSLKMRAVYEVEDGDIVITALPHQVSGAKILAQIAE
                     QMQAKKLPLVADLRDESDHENPTRLVIVPRSNRVDVALLMNHLFATTDLEYSYRVNMN
                     VIGLDGRPQVKDLRMLLTEWLRFRQGTVRRRLQYRLDKVLARLHILAGLLIAYLNIDE
                     VIRIIRYEDHPKAELMKVFGLTDIQAESILELKLRHLAKLEEMEIRKEQNELEKERDN
                     LEHLLANPAAMDKLIIKEIKADMDKYGDDRRSPLVERGEAQALKESDLTPAEPVTVIL
                     SEMGWVRAAKGHDIAVESLSFKGSDKYLTSAQGKSNQPVYFLDSTGRSYSLLANTLPS
                     ARGQGEPLTTKLNPPAGASFKAVVMGESSQAVILATDASYGFVTTLGDLEAGNKNGKA
                     VVTVPEGANIFPPLFVNDVDNDFLAIVSSEGRLLVFAVKDLPKLSKGKGNKLITLDEN
                     ESLKAWIICQAGNKLVINTSNRHLTLKWSDVEHYRGERGKRGAKLPRGFQRVESLAVE
                     V"
     gene            complement(5460..>8432)
                     /locus_tag="PLY05_03500"
     CDS             complement(5460..>8432)
                     /locus_tag="PLY05_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02400.1"
                     /translation="TTATTCTYSNTGLSDMVPTLTYMPNTGIVGSPYYGKFTCTNNGI
                     APATAGATCAISLPAGLSATCSPTVPTAGTVAVGASINCTVTGTPTTSGSGTFVLTTG
                     ATNDSNGGTTTGGNNQVSQAITIVDSTTAPTCSPGSTTNLLSSAGTELYFANNTATPI
                     TNNFGLIASPATYVRTGSNFVVKGNWRWRNANPAAGSPAMTYQLIVNGTVYAQLVGTP
                     SGQEVGTLTALNGATLDGGLSTMQLYDFSVSGLAALPVGVTLPSTVTSISTLSMSFVS
                     STTPADDAGLAPTVLNACALPKLTLSKVSQGGVGAFAFTGNNGFSNETITTTVAGTGV
                     AGTTQTLSAVSTPTTITETIPSGYTVTDISCTGLGSGNATPNLSTGSVTISNTALVYG
                     ANIACTYTNTKLPTITISKISQGGTGTFNFTGTNGFASQNITTSSAGVAVAGATQTLS
                     TAATATTITETIPSGYTVTDISCTGLGAGSATPNLVSGSVSLNAAATAAGANIACTFT
                     NTKLPTITISKISQGGTGTFNFTGTNGFASQNITTSSAGVAVAGATQTLSTAATATTI
                     TETIPSGYTVTDISCTGLGAGSATPNLVGGSVSLNPAATAAGANIACTFTNTASAFTI
                     TGKVFLDNGKNSATPHNGIQEVDEQGIGNALVRLTNCSSTVYQTTMTDGSGGYTLSIP
                     NSLSAGTTLCVEEYQQPANLVSVSGNVGTTGGSYTLATDKVQFTLAASTNYTGVNFGD
                     VVQSQLTGTGNQTIPAGTTASYGHQFIAGTEGSVTFSTAQAPTPLLAWTSLVYLDANC
                     NALLDGGDTQLTSSLNVVADQVVCLLQKVQSPSTATNGSQNVSTLSASFVYAAPSAIN
                     QSLSQIDTTLIMDGGLVLVKKVREVSSCPSTGADTNSFTTNNQALPNALIEYQIVYSN
                     PSANVVSDIVINDMTPSFTTFRSAACYSTPSSVLCSVTSSPSVGGVGSLQWVLTDNPV
                     GLGSGQSGEVRFCVRVDQ"
BASE COUNT         2521 a         1869 c         1596 g         2446 t
ORIGIN      
        1 acggaactgg atgctaaaac agcagagcct attggttttg ttaatttaga tttataactt
       61 atcaaataag ggcttatgtc ataccaatat aagcccttat ttattgtttt atcaggactt
      121 tcccaaatct aaatctctat cccttacccc taacaaatac aaaataccat ctaaacccaa
      181 ggtagaaatg gcttggcgag cattttggcg tactaacggt tttgctctaa aggcaatgcc
      241 taaaccagca attgataaca tgggtaaatc gtttgcacca tcacctacgg caattacctg
      301 ctctaaacgt acaccttctt tggccgcaat atcacgcaac aattcagctt tgcgttggcc
      361 atcaactacc tgtcctacta ctcggcctgt gacttgacca tcaacaatct ctaaatcatt
      421 ggcaaagaca taatcaatac ccaatctatg ctgtaaatat tggccaaaat aagtaaaacc
      481 acctgacaaa atagcggttt tataacccaa ggctttgagt gttttaacca gattttctgc
      541 cccttcggac aattttaatc gtgcagcaat ttcagctaaa acactggcat ttaaaccttt
      601 taataatgca acacgggctt caaaactttg tttaaagtcc aactcccctt gcattgcccg
      661 ctcagtaata gcggcaactt gctcgcctac tccagcggct ttagccagtt catcaatcac
      721 ttcatgttca attaaagtgg agtccatatc aaaacaaacg actcgacgat tacggcggta
      781 ggcatcatct cgttggaagg caatatccat atttaaatca ttggctaaac gtaaaaattc
      841 agcacgcata ctattaatat tgtttggctc accacgtact gaaaactcaa cgcacgcttt
      901 gcctaaacct gcattttcat cttctaatgg cacacgacct gataaacgat ttaaaccatc
      961 aatatttaaa ccattatcag caacaatttg agtgactgcg gccaattgtt cggcagtaat
     1021 tttgcgtgct aataaagtaa cgatgtaacg tgctttacct tgaccactca cccattggtg
     1081 ataactgtct aaggtaatgg gtgtaaaacg caccaataaa ccaatatcat gggcacgaaa
     1141 caacacatct ttcattaata aagccgaatc ttgtgccgaa ggtgattcaa ccaccacacc
     1201 taatgataat tggtcatgaa tcaccgcttg gccaacatcc aaaatattaa ccgcatgctg
     1261 agctaaaacc tccattaaac tggcacttaa acggggtcta tcgggtcccg aaaacgacat
     1321 taaaataatt tcgcgcataa actctactaa attttgtgaa atatcgtgca tgatagggca
     1381 ttgcctaaca atcagctatg cccaatttgt caaagtaatg attataatgg gggattgtta
     1441 cttaatctaa atgtttcttc acttgtcacc tatttaagac tgaggtaagg agtcaaaatt
     1501 gagccaagtt cgtggtattg tgcctttatt tgtggcgagt tattttcttc atgccatgct
     1561 cttggttaat agtgccgaaa cagagctagc caaccaccaa aaaccgattg gtcagttatt
     1621 gagcgagcaa ttagctatta gtgctgctcc tttaatcgct aatcaagata gtgtcggttt
     1681 gggtttattg gccaacaacg ttggttctac agaagctatt cttggtctta aaatatctaa
     1741 cagccaacaa caggttttag ccgcaggtgg acaaggccaa agccaacaag gccaaaattt
     1801 tattgccccg attcaattaa acaacaaagt cttaggctca gtcgaaatta ctttaagcag
     1861 tgcccaacgg ggcgaaatta ttcaacatag tgttgttaat ctcgttttgt cttttgttgt
     1921 gcatctattt ttagcattat ggattggctg gccacaactc tttagcaaca ttcgtattcc
     1981 tattttgcaa gaacccaaag ctccaacgcc agaacccgaa ccgattgtgc ctatcgtaga
     2041 acctgaacct attaaagctg ctgccagtgt ttgggttgcg attggttttg atgatcctaa
     2101 aggtttaatt tctaaagtca atgccaatac aatcaatcaa tttttgcaag ttgttgataa
     2161 attagttaaa cgcaccacac gactttatag tggcaaaatg gtcacacaat taaatagcaa
     2221 tggcgttgtg attcgttttg atggtgacca tgtgtctgat tgtgcccatc gtgcattgtt
     2281 aagtagtcgt ttattaatta aattagtcag caccgcttat ggtcagcgcc gtgaagccaa
     2341 acaatttgcc cttaaagtga aagccagtct cttaaattta gctgagctca cagataatga
     2401 tgccgataaa aaactcaaag ctgtgctgaa tgtcagcaaa gtcaatcaag tgttggtggt
     2461 tgatgacgaa ttgttactca acgatttaaa agctaaacat caactcgttg cctttgaagc
     2521 cagtgaagaa gcctctaaag cggaaaaaga gcttaaggca cacattgttg aagccttagc
     2581 aaccgctcaa gaagaagagc tttcaggatt agaaaaacgg attttagaac gtaaaaaacc
     2641 gaatgcctag aggttatata tgtctgattt tattgccatc gaaacgctga ttaatgacta
     2701 ttttcaggca ttacatagca aagatgtggc taaaattgaa acgatttttt gggatcatgc
     2761 cagcatcaca ggttattacg aaggtgagtt tgttcattcc aagcttcatg attatttgag
     2821 tattttaaag cgcatgtctg cacccaatat gattggtgaa gattttgata tggaagtggt
     2881 cagcattgaa cttaaaggta atatggcggc ggtaaaaacc cgttatctgt ttcaagcttt
     2941 agaatatgtc gattttttaa gtttgttaaa aatagatgat aactggcaaa ttatacaaaa
     3001 gactttttat cacgattaat aaaaaagctc tcctttacgg agagcttttg atgtttctac
     3061 acttctactg ctaaagactc tacccgttga aaacctctag gcaacttagc accacgtttg
     3121 ccacgttcac ctcgataatg ctcaacatcg gaccatttta gcgttaaatg acgatttgag
     3181 gtgttaatca ccaatttgtt gcctgcttga caaataatcc atgcttttag gctttcgttc
     3241 tcgtctaagg taatcagttt attgccctta cctttgctta acttgggtaa gtctttcacc
     3301 gcaaatacca ataaacgtcc ttcactgctc acaatcgcca aaaagtcatt atcaacatca
     3361 ttgacaaaca acggtgggaa aatatttgcc ccttcaggca cagtaacaac ggctttacca
     3421 tttttattac ccgcctccaa gtcgcccaaa gtagtcacaa aaccataact agcatcggtc
     3481 gccaaaatca ccgcttgaga gctttccccc atcacgactg ctttaaaact tgcgcctgca
     3541 ggtggattta atttggtggt taatggctcg ccctgaccac gcgcagaggg taaggtgttg
     3601 gctaataagg aataactacg ccctgtacta tccaagaaat acacaggttg attgcttttg
     3661 ccttgtgctg aggtgagata tttatcacta cctttaaaac ttaaactttc aacggcaata
     3721 tcatggcctt tagccgcacg cacccaaccc atttccgata aaatcactgt gacaggctcg
     3781 gcaggggtta aatcgctttc ttttaaggct tgtgcttcac cacgttcgac cagtggtgaa
     3841 cgtctatcat cgccgtattt atccatatcg gctttgattt ctttaataat taatttatcc
     3901 atcgcagcag gatttgccaa taaatgctct aaattatcac gctctttttc taactcattt
     3961 tgctctttgc gaatttccat ttcttccaat ttggctaaat gacgtagctt taactctaaa
     4021 atcgattctg cctgaatatc ggttaaacca aatactttca ttaattcagc tttgggatga
     4081 tcttcataac gaataatccg aatcacttca tcaatgttta aataggcaat taacaaacct
     4141 gccaaaatat gcaaacgcgc caatacttta tctaaacgat actgcaaacg gcgacgcact
     4201 gtaccttgac gaaagcgtaa ccattccgtc agtaacatgc gcaagtcttt gacttgtggc
     4261 cgaccatcca agccaatcac gttcatgttc acgcgatagc tatattctaa gtcagtggtg
     4321 gcaaataaat ggttcattaa taacgccaca tcaacacgat tagagcgcgg cacaatcacc
     4381 aaacgtgtgg gattttcatg gtctgactca tcacgtaaat ctgccaccaa gggtaatttt
     4441 tttgcctgca tttgttcggc aatttgcgcc aaaatttttg caccagagac ttggtgaggc
     4501 aacgcggtaa tgacaatatc accgtcttcc acttcataca cagcacgcat ttttaacgag
     4561 ccacgacctg ttttatacaa gtttaacagt tcacttttgg cggtaataat ttcggctttg
     4621 gtaggaaaat caggtgcagg aatataagcg gcaatctctt caaggcttaa atttggcttc
     4681 tttaataagg caatacatgc actggccact tcgcgtaaat tatgtggtgg aatatcagtg
     4741 gccatcccca ccgcaatgcc tgttgtgcca tttaataata aattgggtaa acgtgcaggc
     4801 aataaacttg gctcttccat cgtgccgtca aagttaggta cccaatctac tgtaccttgc
     4861 cccaactcag ccaacaacac atcggcataa gcagacaatt ttgactcggt ataacgcatg
     4921 gcagcaaagg acttagggtc atcgggcgaa ccccagttac cttgaccgtc caccaaagga
     4981 taacgataac taaacggctg tgccattaac accatcgctt cataacaggc actatcacca
     5041 tgaggatgat atttacctaa aacgtcaccc accgtacgcg ccgacttttt atgtttagcg
     5101 gagtttttta agcccaattc actcatcgca taaacaatac gccgttgtac aggttttaaa
     5161 ccatcaccaa tatgtggtaa agcacggtct aaaatcacat acatcgagta atttaggtaa
     5221 gcttgttcgg taaattctgc caaagaacgg ctttcggtaa aatctgttac cagtgcgctc
     5281 atagttatct cgtgagtagt ttacgcctga ctttaatgcg tcagtcgtga gttataaaaa
     5341 taatttggcg attttataca gattgacgtg aagaaacagt gttttttttg gttaacagac
     5401 agtttatgac gtgaaaagct tgttcctccc ctaatttagg ggagggttag ggtggggtct
     5461 tattggtcaa ccctcacaca aaaacgcact tcaccactct gccctgagcc taaaccaaca
     5521 ggattatcag tcaacaccca ttgcaaactg cccactccac caacactcgg agacgaagta
     5581 acactgcaca acacactact gggcgtgcta taacaagctg cacttctgaa cgtggtaaat
     5641 gaaggggtca tatcattaat cacaatatca gaaacgacat ttgctgaggg attactataa
     5701 acaatttgat attcaattag ggcattgggt aaagcttgat tattggtggt aaatgaattg
     5761 gtatccgccc cagttgatgg acaactactc acttcacgca cttttttcac caacactaaa
     5821 ccaccatcca taattaacgt ggtatctatt tgacttaaac tttgattaat agcactcgga
     5881 gcagcataaa caaaactagc acttaatgtg gacacattct gtgagccatt ggtggctgta
     5941 cttggcgact gaaccttctg caataaacac accacttgat cagcaaccac atttaacgat
     6001 gaggttaatt gtgtatcacc accatccaac aaagcattac agtttgcatc taaatacacc
     6061 aatgacgtcc atgccaataa aggtgtaggc gcttgtgctg tgctaaatgt aactgaacct
     6121 tctgtaccag caataaattg atggccataa cttgccgttg tgcctgctgg gattgtttga
     6181 ttgccagtac ctgttaattg actttgaact acatcgccaa aattaacccc tgtatagttg
     6241 gtagatgcag ctaaggtaaa ctgcacttta tcggtagcaa gtgtataact gccacccgtt
     6301 gtccctacat taccactcac tgatactaag tttgctggtt gttgatactc ctccacacac
     6361 aaagttgtac cagcacttaa actattggga atactcaaag tatatccacc tgaaccatct
     6421 gtcatagtcg tttgataaac cgttgaacta cagttagtta aacgaactaa ggcattacca
     6481 attccctgct cgtccacttc ctgaatacca ttatgcggtg ttgccgaatt tttaccatta
     6541 tctaagaata ctttacctgt aatggtaaaa gctgaggctg tattggtaaa ggtacacgct
     6601 atattggcac ctgctgcggt tgctgcggga tttaaactaa cactaccacc aactaaatta
     6661 ggtgtcgcac tacctgcgcc taagccagta caactaatat ctgttaccgt ataaccacta
     6721 ggaatggttt cagtaatggt tgttgctgta gctgccgtac ttaaagtttg tgtggcacct
     6781 gcaaccgcaa cacctgcact gctcgtagta atattttgtg aagcaaaacc attggttcca
     6841 gtaaaattaa aggtaccagt tccaccttga gaaattttac taatggtaat cgttggtaac
     6901 tttgtattag taaaagtaca cgctatattg gcacctgctg cggttgctgc agcatttaaa
     6961 ctaacactac cactaaccaa attaggtgtc gcactacctg cgcctaagcc agtacaacta
     7021 atatctgtta ccgtataacc actaggaatg gtttcagtaa tggttgttgc tgtagctgcc
     7081 gtacttaaag tttgtgtggc acctgcaacc gcaacacctg cactgctcgt agtaatattt
     7141 tgtgaagcaa aaccattggt tccagtaaaa ttaaaggtac cagttccacc ttgagaaatt
     7201 ttactaatgg taatcgttgg taactttgta ttggtataag tacacgctat atttgcgcca
     7261 tacaccaatg ccgtattact aattgtgact gagcctgtcg ataaatttgg tgttgcatta
     7321 cctgaaccta agccagtaca actaatatcg gttaccgtat aaccactagg aatggtttca
     7381 gtaatggtcg ttggtgtcga tacagcactt aaggtttgtg tggtacctgc tacacctgta
     7441 cctgcgacag tagtagtaat cgtttcatta ctaaaaccat tattaccagt aaaggcaaaa
     7501 gcacctacac ctccttggct aactttgctt agtgttaact ttggtaaagc acaggcattt
     7561 aatactgtcg gtgctaggcc agcatcatcc gcaggagtag tagaactgac aaaagacata
     7621 cttaatgtag aaatactggt tactgtagaa ggtaaggtta ccccaactgg caaggcagct
     7681 aaaccagaca cactaaaatc ataaagctgc attgtgctta aaccaccatc taaggttgca
     7741 ccatttaatg cagttaatgt acctacctct tgtccactag gagtacccac taattgtgca
     7801 tacacagtgc cattaacaat taactgataa gtcattgcag gagagccagc cgcaggattg
     7861 gcattacgcc aacgccaatt acctttaacc acaaaattac tgcctgttcg tacataagtc
     7921 gcggggcttg caattaaacc aaagttattg gtaatagggg ttgcagtgtt atttgcaaaa
     7981 tacagttctg taccagcaga gcttaataaa ttagtagtac taccaggcga acaagtcggt
     8041 gcagtcgttg agtcaacaat ggtgattgct tggctaactt gattattacc acctgttgtt
     8101 gtaccaccat tgctatcatt agttgcgcca gtagttaaca caaatgtacc agaacctgat
     8161 gttgttggtg tgcctgtgac cgtacaatta atagatgcac caaccgcaac cgtgccagcc
     8221 gttggcactg ttggactaca agttgcactt aaaccagcag gcaagctaat tgcacaagtt
     8281 gctcctgctg ttgctggcgc aataccatta ttagtacagg taaatttgcc ataatagggt
     8341 gagcctacaa tacctgtatt cggcatatag gttaaagttg gcaccatatc ggataaacct
     8401 gtattggagt aagtacaggt tgtcgctgtt gt
//
LOCUS       DAOQMB010000057         8350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3416_length_8350_cov_16.846896, whole genome shotgun sequence.
ACCESSION   DAOQMB010000057 DAOQMB010000000
VERSION     DAOQMB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_8350_cov_16.846896"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLY05_03505"
     CDS             <1..189
                     /locus_tag="PLY05_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HNC02401.1"
                     /translation="ADLIMFIYRDEVYNPESKDKGTAEIIIAKQRNGPIGTSRLAFLG
                     HLTKFGDLAPEYYRPDDE"
     gene            189..1265
                     /gene="alr"
                     /locus_tag="PLY05_03510"
     CDS             189..1265
                     /gene="alr"
                     /locus_tag="PLY05_03510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC02402.1"
                     /translation="MRDAQLIINRDALLHNLQQVKHHAPQAKVLAMVKADAYGHGALL
                     IAQTLAPHVDALGVAFLDEALALRQAGISCPIAILEGVFNQSELEQALAAQCYLVVHQ
                     QQQLDLLANYQGTDQTHIWLKIDSGMHRLGFAPELALSAYQQLSTLNCVQEIILTSHF
                     ACADDPSSIKTQQQIDVLNQLYQQLPQTLSRSFANSAAILAWPNTHHQWVRPGIMLYG
                     SSPFIHKTAKKLNLQAVMTLKTKIIALRTINQGETVGYGSTWQASRKTRLAVIAIGYG
                     DGYPRHINANTPVLINGQRCFIVGRVSMDMITVDVTDLNVDLGDEVILWGQDLSADEI
                     AGYAQTISYELFCQITPRVKRLVT"
     gene            complement(1483..2130)
                     /locus_tag="PLY05_03515"
     CDS             complement(1483..2130)
                     /locus_tag="PLY05_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02403.1"
                     /translation="MLIKKGYYVVLLALVISACSNSEEAPTQNNQPKTPPVIEAPVVQ
                     SEPEPYQLPKRPDTPDPILTIADVKAMTSPDQLAKALVAPDAQVRLAAVQALSPYAAN
                     LPDVALQRLINMLDADQDKLIVKNVALLLSQTCHPAVMVMLLNNLNRDINSLNIEALT
                     VLGDVAGYRAVEKIDQLLARLENEQSITAEQIRPIAQQAKEKIIARNGKPLACSW"
     gene            2274..3626
                     /gene="radA"
                     /locus_tag="PLY05_03520"
     CDS             2274..3626
                     /gene="radA"
                     /locus_tag="PLY05_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNC02404.1"
                     /translation="MSKIKTSYSCHACGSSFSKWAGQCSECGAWNSIHEVKADTSLSP
                     RQRGNYAGQASTVTILDQVSIDAIDRTHTGLSELDRVLGGGLVAGSVVLIGGDPGIGK
                     STILLQTLTHLAQQHNALYITGEESLSQVAMRGKRLELPCDKVKIMAETCVENIVATL
                     RSEKPAVAVIDSIQTLYTEALQSAPGGVSQVRESAALLTRYAKQSGTALFLVGHVTKE
                     GSLAGPRVLEHMVDCVLYFEGESDSRFRMIRAVKNRFGAVNELGVFAMTDRGLREVAN
                     PSAIFLSRYEEPIAGSVVMVSREGTRPLLVEVQALVDDSFANPRRVAIGLDQNRLAML
                     LAILHRHGGVHTMGQDVFVNVVGGVKVLETGSDLAVLLAVTSSLRGQALSADLAVFGE
                     VGLSGEIRPVPNGQERLREAAKHGFKRAIVPKANVPRQTIEGMEVIGVGRLHEALAAV
                     "
     gene            complement(3623..4129)
                     /locus_tag="PLY05_03525"
     CDS             complement(3623..4129)
                     /locus_tag="PLY05_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC02405.1"
                     /translation="MPSGHILFAIHHGTYVLKFTGEVRAPMCATLDDFLEKMFVDSEL
                     SAILVDLTETDYIDSTALGMIAKTAVFLQMHNQRKPIILSTNPDITRLLESMGFDQVF
                     IILACECHEECLNELPESEPSEHEMMEKVISAHRVLMGLSEQNQATFKNLVEALELEQ
                     QSHGEDKI"
     gene            complement(4131..5306)
                     /locus_tag="PLY05_03530"
     CDS             complement(4131..5306)
                     /locus_tag="PLY05_03530"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNC02406.1"
                     /translation="MIHFQQDCGFILVVYPNAETRQWLVDVLEHIGHTVVSEPNLEAA
                     IINFQANHQQLLLVDLSLIEGDTLNHFAQDNPETPIVTVSNDVEMDQVLEGLKNGAID
                     HFFLNRGIVVLDHTVKRALERGRLLKQNREYQQQLEAANAELARSLETLQEDQEAGRR
                     IQFRLLPQTPYQAFADCIVTHRILPSLYLSGDFIDYFKIGEGKLGFYLADVSGHGAAS
                     AFVTVFLKAITNRIQKHYERKTTVNVVAPARILAMINDELIAARMGKHLAMFCGVIDF
                     EHNRFTYSVAAHYPPPILINNGRVMSLGGRGLPLGLFKEVHHEEHTMDLEPHFTFIAT
                     SDGVLDVLPKGSALEKEKRLQAMAMNVKNVDDLVKELNLSEDDPLPDDVALLVINRV"
     gene            complement(5336..6055)
                     /locus_tag="PLY05_03535"
     CDS             complement(5336..6055)
                     /locus_tag="PLY05_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HNC02407.1"
                     /translation="MFRHLANACVCTLIVISSQHAIATENPDPWENVNRKIFAFNKNA
                     DTYVLKPIARGYRAATPDIVDNSITRFFDNLQQPLVIINNGLQGKGQQAASDTGRFLV
                     NTITSLGFADVASHMGLVKHEEDFGQTFGKWGASSGAYVMLPFLGPSSVRDALAKPLD
                     MLVNPRNLLDDAPLNMALAGLQVVDTRADLIPIEKIIEGDEYLFLRDIYLQRREFLVQ
                     DGKVKDSFMDDMDDESNEAAQ"
     gene            complement(6212..6937)
                     /locus_tag="PLY05_03540"
     CDS             complement(6212..6937)
                     /locus_tag="PLY05_03540"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HNC02408.1"
                     /translation="MSDFVALQQQWTNWLRHPQTSPQPQIEQRRLTIYRELFFNNVSS
                     FVNSAFPLLQQQLSTEVWQQLLDDFFANHHCQSPYFYDISSEFYQYLATQTYLLADYP
                     WLLELAHFEWAEIAADIAEGVWPDYQQGDLLTNIPVLNPFVWPLVYQWPVHKMNKDKN
                     NLSSVTYILMYRNLENDVQMMELNPLSLQVVQLLQDNQTMTGHDLLDILAQQLQQSQR
                     ALEVPFKQLLATLLDKQIILGVI"
     gene            complement(6934..7767)
                     /locus_tag="PLY05_03545"
     CDS             complement(6934..7767)
                     /locus_tag="PLY05_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002048427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HNC02409.1"
                     /translation="MSKKIYGSGLGLRRSFMRELLESESQLSMPDFFEVAPENWIGMG
                     GRYRQQLMAYGERFPIVAHGLSLSIGGLKPLDIEFINNIKNFIQDFDIKCYSEHLSYT
                     GDEGHLYDLLPIPFTEEAVKHVAQRIQQVQDILQQRIALENVSYYAAPCQDLSELEFL
                     LAVITEADCDLLLDVNNVLVNSINHSYQAINFIDKLPTERIRYLHIAGHYVEAEDLRI
                     DTHGDDVCADAWQLLEYTYQRHGVLPTLLERDFNIPPINELLQEVEIIRDYQRQGANK
                     K"
     gene            complement(7802..8230)
                     /locus_tag="PLY05_03550"
     CDS             complement(7802..8230)
                     /locus_tag="PLY05_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02410.1"
                     /translation="MKLSSLTSVAAVLAATVTVPVQADNNPFVAKPLSSMTIASNHTN
                     HEGKCGEGKCGGSMKAEEKAKEGKCGEGKCGGKMKNPPADTNKDGNVSKQEFMTRHEQ
                     MFNEADTNKDGMLSADERKGLHKKMMKEGKCGEGKCGSSK"
BASE COUNT         2514 a         1694 c         1491 g         2651 t
ORIGIN      
        1 gctgatttaa ttatgtttat ttatcgagat gaggtatata atcccgaatc aaaagataaa
       61 ggcactgccg aaatcattat tgctaaacaa cgtaacggcc caattggtac ttcacgctta
      121 gcgtttttgg gtcatttaac caagtttggt gatttagctc ccgaatacta ccgccctgat
      181 gatgaataat gcgtgacgct caactaatta tcaaccgaga tgctttatta cataatctgc
      241 aacaagtaaa acatcatgca ccacaagcca aggttttagc gatggttaaa gccgatgctt
      301 atggtcatgg tgcgttattg attgcccaaa ccttagcacc tcatgttgat gcgttgggcg
      361 ttgcattctt agatgaagcc ttagcgttac gtcaagcagg catttcttgc ccgattgcta
      421 ttttagaagg tgtctttaat cagtctgaat tagaacaagc cttggctgcc caatgttatt
      481 tggttgttca tcaacaacag caacttgatt tattagccaa ttatcaaggc acagaccaaa
      541 cacacatttg gttaaaaatt gatagtggca tgcaccgcct aggttttgca ccagaacttg
      601 ctctatctgc atatcaacaa ttatcaaccc tgaactgtgt ccaagaaatt attttaacca
      661 gtcattttgc ttgtgctgat gatccgagtt caatcaaaac ccaacaacaa attgacgtgc
      721 ttaatcaact ttatcaacaa cttcctcaaa ctttatcccg tagttttgcc aattctgcgg
      781 caattttggc ttggccaaat actcatcatc aatgggttag accggggatt atgctctatg
      841 gtagctcccc ttttattcat aaaacagcca aaaaattaaa cctacaagcc gtgatgacgc
      901 taaaaaccaa aatcattgcc ttacgcacaa tcaatcaagg ggaaaccgta ggttatggca
      961 gcacatggca agcctcacgc aaaactcgtt tagcagtgat tgctattggt tatggcgatg
     1021 gttatccgcg ccatattaac gccaatacgc cagtgttaat taatggtcaa cgttgtttta
     1081 ttgttggtcg agtgagtatg gatatgataa cagttgatgt gactgatctt aatgttgatt
     1141 taggtgatga agtgatttta tggggacaag acctgagtgc tgatgaaatt gctggctatg
     1201 cccaaaccat tagttatgag cttttttgtc aaatcacacc aagagttaaa cgtttagtca
     1261 cttaaagcat atcaatatat tttttagact aaaacaatct atcaactttt ttatcatcag
     1321 ctatatttaa attgaaggtt aaatttttta ggagaaaaca aattataagt cgctctttaa
     1381 aacaatttgt cttttaatat ttaaggtggc aacgccttta tagtccgtca tgcagacgaa
     1441 ccaaccccac aaactctacg gtttagctgg ggtttttaat ttttaccaac tgcacgccaa
     1501 aggtttacca ttgcgcgcta taattttttc tttagcttgt tgggctatag gcctaatttg
     1561 ctcggctgtt atactttgct cattttctaa acgtgctaat aattgatcaa ttttttcaac
     1621 agcacgatac cccgcaacat cacccaatac tgttaatgct tcaatattaa ggctatttat
     1681 atcacgattt agattattca ataacatcac catcaccgct ggatgacaag tttgactcaa
     1741 taacaatgcg acgtttttga caattaattt atcttgatca gcatctaaca tattaatcaa
     1801 acgctgcaat gcaacatctg gtaaattcgc tgcataaggg gaaagtgctt gaacagccgc
     1861 taaacgcacc tgtgcatctg gtgcaactaa ggctttagct aactgatctg gactggtcat
     1921 tgctttaaca tcagcaatcg tcaaaatggg atcaggggta tcgggtcttt tgggaagttg
     1981 ataaggttct ggctcacttt gcacaacagg agcttcgata acaggtggtg tttttggctg
     2041 attgttttgt gtcggtgctt cttccgaatt actacatgcg gaaatgacta aagccaataa
     2101 aacaacgtaa tacccttttt ttatcaacat gtttcttaaa tccactgata agcgacaatt
     2161 ctagtcgcat ctactttgct tattttaaca aaaccacgta acctaatacg atcaatttat
     2221 ttatgccaaa gcttaccatg agttgctaag cttcacttat attgcctaaa attatgagta
     2281 aaatcaaaac ttcttatagc tgtcatgctt gtggtagctc tttttctaaa tgggctggcc
     2341 aatgcagtga atgtggtgca tggaatagta tccacgaagt taaagctgat actagcctca
     2401 gccctcgcca acgtggcaac tatgcaggtc aagccagtac cgtgactatt ttagatcaag
     2461 tcagtattga tgccattgat cgcacacata caggactttc tgaattagac agagtcttgg
     2521 gaggcggctt agtggctggt tctgtcgtat taattggcgg tgatcctggt attggtaaat
     2581 caacgatttt attacaaaca ttgactcatc ttgcccaaca acataacgcc ctttatatca
     2641 cgggtgaaga gtctttatca caggttgcca tgcgcggcaa acgcctagaa ttaccgtgcg
     2701 ataaagttaa aatcatggcc gaaacctgcg ttgaaaacat tgttgccact ttacgcagcg
     2761 aaaagccagc cgttgcggtg attgactcga ttcaaacctt atataccgaa gccttacaat
     2821 ctgcaccagg tggtgtttct caggtacgtg aatcggctgc gttattgact cgttatgcca
     2881 aacaaagtgg cactgccctg tttttggtgg gtcatgtgac caaagaaggc tctttggctg
     2941 gccctcgggt cttggaacat atggttgatt gtgtattgta ttttgaagga gagtcagact
     3001 cacgttttag aatgattcgt gctgtcaaaa atcgttttgg tgcagtcaat gaattaggcg
     3061 tgtttgccat gactgatcga ggtttacgcg aagtagccaa tccttcagcg atatttttat
     3121 cgcgttatga agaaccgatt gcaggttcag tggtcatggt tagccgtgaa ggtacacgcc
     3181 ctctactcgt agaagttcaa gccttagtcg atgactcctt tgctaatcct cgccgtgttg
     3241 cgattggtct agatcaaaat cgtttagcga tgttattagc cattttgcat cgtcatggtg
     3301 gtgtacacac catgggtcaa gatgtgtttg ttaacgtggt tggtggtgtg aaagttttag
     3361 aaactggctc agatttagcc gtattattag cggtgacttc tagcttacgt ggtcaagcat
     3421 tatctgctga tttagctgta tttggcgaag ttggtttgtc tggcgaaatt aggcctgtcc
     3481 ctaatggtca ggagcggtta cgcgaagccg ccaaacatgg ttttaaacgt gctattgtgc
     3541 caaaagccaa cgtgccacgc caaacgatcg aaggcatgga agttattggc gttggtagat
     3601 tacatgaagc attagctgct gtttagattt tatcttcacc gtggctttgt tgttctaact
     3661 ctaaagcctc aacaagattt ttaaaagttg cctgattttg ttcgcttaaa cccattaata
     3721 ctctatgggc tgatataact ttttccatca tttcgtgttc ggaaggctct gattctggca
     3781 attcatttaa acattcttcg tgacattcac atgccaaaat aataaatact tgatcaaagc
     3841 ccatgctttc taatagtcga gtaatatctg gattcgttga caaaataatt ggtttacgtt
     3901 gattatgcat ttgcaaaaaa acggctgttt ttgcaatcat tcccaatgcg gtactatcaa
     3961 tataatcagt ttcggttaaa tcaactaaga ttgcagataa ctcactatca acaaacattt
     4021 tttctaaaaa gtcatctaag gtcgcacaca tcggtgcgcg tacctcacct gtaaatttta
     4081 gaacataagt accatgatga atggcaaata aaatatggcc tgaaggcata ctacactcga
     4141 ttaattacaa gcaaagcgac atcgtctggt aaaggatcgt cttcgcttaa atttaattct
     4201 ttcactaaat catcaacatt ttttacattc attgccatcg cttgcaatcg cttttccttt
     4261 tctagtgccg accccttggg caacacatca agcacaccat cagatgttgc aataaatgta
     4321 aaatgtggtt ctaaatccat cgtgtgttct tcgtgatgta cctctttaaa taagcccaaa
     4381 ggtaaacctc ttcctcctaa agacatcact cgaccattgt taattaaaat aggtggtgga
     4441 taatgtgccg ccacactata agtaaaacga ttatgctcaa agtctatcac accacagaac
     4501 attgctaaat gctttcccat acgagctgcg attaattcat cattaatcat ggctaatatt
     4561 ctagcaggtg ctacaacatt taccgtagtt ttacgttcgt aatgtttttg aattctattg
     4621 gtaattgctt ttaaaaaaac cgtcacaaag gcacttgctg caccatgacc tgatacatcg
     4681 gctaaataaa agcctaattt gccttcacct attttaaaat agtcaataaa atcacctgat
     4741 aagtataatg aaggcaaaat acgatgcgtg acaatacaat ctgcaaaagc ttgatatggt
     4801 gtttgcggta ataatctaaa ttgaatacga cgacctgctt cttgatcttc ttgcagggtt
     4861 tctaaacttc tcgccaactc agcgtttgct gcttctaatt gctgttggta ttctcgattt
     4921 tgttttaata aacgcccacg ttccaacgca cgttttacgg tatgatctaa aacaacaatg
     4981 cctctattaa gaaaaaaatg gtctattgcc ccatttttta aaccttctaa aacttgatcc
     5041 atttctacat cattcgaaac ggtcactata ggtgtttcag gattatcttg agcaaaatga
     5101 ttaagcgtat cgccttcaat caaactcaga tcaaccagta aaagttgttg atgattggct
     5161 tgaaaattta taattgctgc ttctaggttt ggctcactaa caactgtatg gcctatatgc
     5221 tctaatacat ccaccaacca ctgacgagtc tcggcatttg gatatacgac taatataaaa
     5281 ccacagtctt gctgaaagtg aatcattttc ttaaattcct aaaacagaac ccatgttact
     5341 gggctgcctc atttgattca tcatccatat catccataaa actgtcttta accttgccat
     5401 cctgcactag aaattctcta cgctgcaaat aaatatctct taaaaataaa tattcatcgc
     5461 cttcaataat tttttctatt ggaattaaat ctgctcttgt atcaacaact tgtaatcctg
     5521 ctaaagccat attcagtgga gcatcatcta acaaattgcg tggattaacc aacatatcta
     5581 aaggcttagc caaggcatct cgtacagaac ttgggcctaa aaatggcaac atcacataag
     5641 cacccgatga agctccccat ttaccaaaag tttggccaaa atcttcttca tgtttgacta
     5701 aacccatatg gctagcaaca tcagcaaagc ccaaactagt gattgtatta accaaaaaac
     5761 ggcctgtatc acttgcagct tgttggcctt ttccttgtaa gccattattg attatcacta
     5821 aaggttgctg aagattatca aaaaaacggg taatactatt atcaacaata tcaggagtag
     5881 cagctcgata accacgcgca ataggtttta acacatacgt atctgcattt ttattaaatg
     5941 caaatatttt acgatttaca ttttcccaag gatctggatt ctcagtggca attgcatgtt
     6001 gagagctaat aactataagt gtacacacac aagcgttggc taaatgacga aacataccct
     6061 ctgctctata tttatttgtt gtgggctgat tgtaagcaag aacgatagca aaaagaacca
     6121 gttttattgt ctttatttgt gttttggatg aaaaaaattg attttacgca cttttttaca
     6181 attttgatga ttttatcagt ctcaatgatt cctagataac gcccaaaata atttgtttat
     6241 ctaataaggt agccaataat tgtttaaaag ggacttcaag ggctcgttgt gactgttgta
     6301 attgctgagc taatatgtct aacaaatcat gacctgtcat cgtttgatta tcttgtagca
     6361 attgaacaac ctgtaaagat aaaggattca actccatcat ttgtacgtca ttttctaaat
     6421 ttctatacat caaaatataa gtgactgatg acaaattatt tttatcttta ttcattttgt
     6481 gaacaggcca ttgatacacc aaaggccaaa caaaggggtt taagacagga atatttgtca
     6541 acaaatcgcc ctgttgataa tcaggccata cgccttcggc aatatcagca gcaatttctg
     6601 cccactcaaa atgagctaat tctaataacc aaggataatc cgccaataag tacgtttgag
     6661 tagctaaata ctgataaaac tcagatgaaa tgtcataaaa atagggactt tgacaatgat
     6721 gattggcaaa aaaatcgtct agcaattgtt gccatacttc ggtgctgagt tgttgttgca
     6781 gcaacgggaa agcactatta acaaaactac tgacattatt aaaaaacaac tcacgataaa
     6841 ttgttaaacg tctttgttca atctgtggct gaggggatgt ttgaggatga cgtaaccagt
     6901 ttgtccattg ttgttgtaaa gcgacaaaat cactcatttt ttgttcgctc cttgacgttg
     6961 gtaatcacga ataatttcta cttcttgtaa cagttcgtta atcggcggaa tattaaaatc
     7021 gcgttctaat aatgttggta acacaccatg tctctgatag gtatattcta ataattgcca
     7081 agcatcagca cagacatcgt caccatgtgt atcaatgcgc aaatcttccg cttcaacata
     7141 atgccctgca atatgcaaat aacgaatacg ctctgtaggc aatttatcaa taaaattaat
     7201 agcttgataa gaatgattaa tgctatttac taaaacatta tttacatcta acaataaatc
     7261 acaatctgct tcggtgatga ctgctaataa aaactctaat tcacttaaat cttggcatgg
     7321 tgcggcataa taagagacat tttctaaagc aatgcgttgt tgcaagatat cttgtacttg
     7381 ctgaatgcgt tgggcaacat gtttaactgc ctcctccgta aacggaattg gtaataagtc
     7441 atacaaatgc ccttcatcac ccgtataact taaatgttct gaataacatt taatatcaaa
     7501 gtcttgtata aagtttttaa tgttatttat aaactcaata tcgagtggtt ttaaaccgcc
     7561 aattgacaaa gacaaaccat gagcaacgat gggaaaacgc tcaccataag ccattaattg
     7621 ttgacgataa cgccccccca tacctatcca attttcgggg gcaacctcga aaaagtcagg
     7681 cattgataat tgagactccg attctaatag ctcacgcata aaactgcgac gcaatcccaa
     7741 accagagcca taaatttttt tactcatatt atcaatacct taaccatctt ttctaatgta
     7801 actatttaga tgaaccgcat ttaccctcac cacatttgcc ttctttcatc atttttttat
     7861 gtaatccttt acgttcatca gcacttaaca taccatcttt atttgtatct gcttcgttaa
     7921 acatttgctc atgacgtgtc ataaactctt gtttagaaac attaccatct ttatttgtat
     7981 ctgctggtgg gtttttcatt tttccgccac acttgccttc gccacatttg ccctcttttg
     8041 ctttttcttc ggctttcata ctaccaccgc atttgccctc accacattta ccttcatgat
     8101 tggtatgatt acttgcaata gtcattgagc taagaggttt agcaacaaag ggattattat
     8161 cagcttggac aggtacagtt actgtcgctg ctaacacagc agcaacagat gttaaagaag
     8221 ataatttcat tatggtttac tccaatattt tttgactaaa agagacatca agttatctct
     8281 gattatttag agtaaataaa ggccgatttt gttacattta tttatttttt gcacaataaa
     8341 aaagagacac
//
LOCUS       DAOQMB010000058         8304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3451_length_8304_cov_17.880955, whole genome shotgun sequence.
ACCESSION   DAOQMB010000058 DAOQMB010000000
VERSION     DAOQMB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8304
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_8304_cov_17.880955"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLY05_03555"
     CDS             complement(<1..626)
                     /locus_tag="PLY05_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02411.1"
                     /translation="MLAYNKLTRTLCCIAAIYMAQGVHAEEAQNKQDTPKVAETLATP
                     QQDRISDVVIGRDLETLKTAQSRIAKLNNNGIEAENYFLVKAQAWLDFAMHEYYENDR
                     SQVIENALAESYKLIKEMEAANNSISMDTLVIPESVRLREDLWKIAAELKQHQGFVCA
                     AAPIAEMEVRLVWAGHEHEELGWRHAREHFAAAERLAKKARKLADNCF"
     gene            complement(613..8142)
                     /locus_tag="PLY05_03560"
     CDS             complement(613..8142)
                     /locus_tag="PLY05_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02412.1"
                     /translation="MCLSTCANQLHNSSKNKGYIVYFLNYSTQVIYMLRTLVALCMML
                     CGVAYAQMPAAGYQIKNEAFAQFVNETGETYLLKSQPVIVQVLPVLAATLTPANDIVA
                     SPSQVVFWPHILTNTGNADDGYRFSLTDVGGDSGILIKPQLIHDVNKNGAFDAGDVII
                     DPEAHLEQIAVGQSMSLLVRAQLPATLLPGSQFSVSLKVFGSQGVPVLQQTDNVYTNA
                     PQFVLSKNVNKTQINLNNPSNTLLYSLEFSNTGNAVALGTTVSIDGVNQQKVVFDDPL
                     PVGVTFKDIYLRKGGGELLYHLKGESQQVYHRYNSGTLPSLTQIDRIAVAYSSFDVKQ
                     EGQIDVEVEVTTQQLSNLNNQFVSYYKYGNEEKQAFSNVVTTLLQKNSFSLLIDNSRV
                     AKLNDVVALPHVLQNNGSFAESYTLSVTQLTNDNSDLLNVIIVQDINNNERYDVGIDQ
                     QITELRLDANKSAKLLLVGTVPSDLANQDKIKLTLTAQSKNTNQQQSVNDELIINSSL
                     GSGLDMSLTDALTDANSQQNPPSVVANQPVIWKHILKNTGSLADSYDILVSNLADDNK
                     DIANLQVFIDSNNNGVYDKDVDTEVSSAIPLNPQQQVTLFVIGTTPADAKLNDKINIA
                     LQTKGVRSGLIKNTIDTVSVASASLSSKNNDYTIDKQKVLVKAVDSDGIYLEAGVAQC
                     NTRANVADKVLLRVRSFALEDSATIIDEEFVIAEEASDKTDSSLFRLSNPLKTQSRRD
                     QKTRQLINVTKENQILEVLTTDKVEVTIIHCLEDDGKTIKKDLQNQEMKIYKDFATKE
                     QVKTNIQIFELASLESKNASYATLRRKILANQKLFLETQTEQCNTRIGVADKLLLRVR
                     SLIKDNQGKDSSIFDEEFVVAIETGNDTGLFRLEKPLPTRSRLENTPIKQNEILETIA
                     HDTVRATIMHCLDDDGKTIKKDEQGLEMKVYKDPINQVDEVSTELLVDPFGVVFDSKT
                     GKLLNGATVKLIRVENGKEAPFKLFDDNGKECEVDTEASPSCKKTIITGTLDINGDGV
                     VDAGDDGMFRFPFVPATNAISSFYRLVITAPDGYTYPSVVSPTALLNNPATKNRVIHS
                     TGDFTTGDDDGSYGGRFNVLPTDGPVELDIPMDPPAIDKASLFIKKESNRKNAELGEF
                     VDYTVVVRNVSVNKTVATQVVVKDTLPKGFSYVQDSGRVDKVQQNPQGGIGPVLSFTV
                     GDLAADAEVKLTYRAHIGTGALHGDGINRVIATDSTGASSQEAVAAVKVTAGVFSQEA
                     FVIGKVYTDCNRDGVQGLEEIGVPNVRLILEDGTYVVTDVEGKFNFYGLRPTTHVLKL
                     DRTSLPEDVELIEQNVRNAGDPSSRFVDLKSGEIHRADFAITTDNATCSGPAMAEIYA
                     RRNQGDKAIGELERELKADLTFERNNPSDVRSLPANGCVNYTGNECGLHGKAKMPTLT
                     TAVVKADQNSQEDDINRIELAKDNRSLESYLDNTSSNQLDILNLVDGQVLPYSQTRIL
                     FKGVLGARFELSVNEQVLDEKSMGIRMAVTEQRLEAREYIGINLKAGQNRIVLKQFDG
                     LGNVRGTRSLVVTAPANLAKLLVTTDQKVVEANGSNEVLVSVKLQDEAGVPITTRTSI
                     TLESNAGRFRAKDLDPKTQGIQLFVEGGEFSVKLVAPTQAGESTIRVSSGVLKTEQNI
                     RFVPELRPMVAAGIVEGMVTFKNFDSAKVQPAYANDGFEAELQNLSSSNDGKLNVGGR
                     AAMFLKGKVKGEYLLTLAYDSNKEKDQRLFRDIRPDDYYPVYGDSAVRGFDAQSTSKL
                     YVRLDKGRSYIMFGDYTTRIEGTEALSLGQYNRSLTGIRGHYATDTFKATTFVAQTNA
                     KQRVFEIPAQGISGPYVLPSLDGLKVNSEKVEIIKRDRNNLGLIINTEAKTRFTDYEF
                     EALTSSIYFKSPVPSLDANLNPYAIRVTVEVEEVAGEDYVVGGVQVEQKLGEKITVGA
                     AAVQEDVPNDNYQLASANLTLQLTEATKLIAEVAQSDRETKALGQASRAELLHSTPRI
                     NARVYYGESDTAFENPAAPLTSGRAESGIKATINMDSWGNLLTEAIRTENVTTGGVRH
                     GVKVTISRSFAQHFTAELGGRFYDETALPANNTNLGVTPYEGVTVHSKLNIQIPQLDG
                     ASVYGEYEQDIENSDRKVISIGGDYQINSQSKLYAKHELSSSVNGGFGLNDQQQRNNT
                     VIGVESNYMKDGRVFSEYRVRDAINAEDVEAAIGLRNSWYLTEGLRLNTNFEEVRTLD
                     GDSNSDAIAASLGVEYLANPLWKATGKIDLRWADSADSILNTLGFAYKISRDWTLLTK
                     NMLSLTDNHGVNVGNRVQNRFQLGAAYRQVDINRWDMLTKVEYKLDDNKATLNAHSKT
                     DSYIFSNHINYHPTRRWTFASQLAAKWYTDNSSNLDSEATTYLIGGRIIHDISERWET
                     SIQAGWLSGDLGGQRTVLGAEAGYLVTTNLWLSLGYNWLSYDDADLVGTDFTVDGAYM
                     RLRFKFDEDLFGGNKPSTNKALEPKNVGL"
BASE COUNT         2523 a         1811 c         1323 g         2647 t
ORIGIN      
        1 cagaaacaat tatcagctaa tttgcgcgct tttttagcta atctttcggc tgcggcaaaa
       61 tgctctctag catgtcgcca tcctaactct tcatgttcat gacctgccca aaccaaacgc
      121 acttccattt ctgcaatcgg tgcagcagca cagacaaagc cttgatgttg ctttaattcg
      181 gcagcaattt tccataaatc ttcacgcaaa cgcactgact caggaatcac taaggtatcc
      241 atagagatac tattattagc ggcttccatt tctttaatga gtttatacga ttctgctaaa
      301 gcattctcga tcacttggct acggtcattt tcataatatt cgtgcatagc aaaatctagc
      361 caagcctgcg ccttaactaa aaaataattt tccgcttcaa taccattatt atttaatttg
      421 gcaattcgac tttgtgcagt ttttaaggtc tccaaatcac gaccaatcac cacatcacta
      481 atacgatctt gctgaggagt tgctaaagtt tcagctactt ttggcgtgtc ctgcttattt
      541 tgcgcttctt cagcatgaac accctgagcc atataaattg cagcaataca gcacaatgtt
      601 cgtgttaatt tattataggc caacattttt gggctccaat gctttattgg ttgaaggctt
      661 attgcctcca aataaatcct catcaaactt aaagcgtaaa cgcatataag ccccatctac
      721 ggtaaaatca gtacctacta aatcggcatc atcatagctt aaccagttat atcccaacga
      781 cagccataaa ttagtagtca ctaaataacc tgcctccgca ccaagtaccg tacgttggcc
      841 acctaaatca ccacttaacc atcctgcttg aatgctagtc tcccaacgtt cactaatatc
      901 gtgaataatt cgcccaccaa tcagataggt tgtcgcttcg gaatctaaat tactcgaatt
      961 atcggtatac catttggctg ctaattggct tgcaaatgtc cagcgacgag taggatgata
     1021 attaatatga ttagagaaaa tataactatc tgttttgcta tgagcattta acgttgcttt
     1081 gttatcatct aatttgtatt caactttggt caacatatcc caacgattaa tatcaacttg
     1141 acgataagct gcacctaatt gaaaacggtt ttgcacacga ttgccgacat tcacgccatg
     1201 attatcggtc aaactcaaca tatttttagt caacaatgtc caatcacgag aaattttata
     1261 agcaaaaccg agtgtattta aaatactatc ggcactatca gcccaacgta aatcaatttt
     1321 acctgtcgct ttccacaatg gattagctaa gtattccacg cccaaactcg cagcaattgc
     1381 atcactatta ctatcaccat ctaaggtacg tacttcttca aaattggtat ttaaacgtaa
     1441 accttctgtt aaataccagc tattacgcaa gccaatagcg gcctcaacat cttcggcatt
     1501 aattgcatca cggacacgat attcactaaa tacgcggcca tctttcatgt aattactttc
     1561 tacaccaatg accgtattat tacgttgttg ttgatcattc agaccaaagc caccattaac
     1621 cgatgatgat agttcatgtt tagcgtataa cttactttga ctattgatct gataatcacc
     1681 accaatagaa ataaccttac gatcactatt ttcgatgtcc tgttcatact caccataaac
     1741 actcgcgcca tctaattgag gaatttgaat gtttaatttg ctatgcacag tcacaccttc
     1801 ataaggagtc acccctaaat tagtgttatt ggcaggcaaa gcggtttcgt cataaaaacg
     1861 accacctaac tctgcggtaa aatgctgtgc aaatgaacgg ctaattgtta ctttaacgcc
     1921 atgacgtaca ccacctgttg tcacattctc ggtacgaatc gcttcagtca acaaattacc
     1981 ccatgaatcc atattaatgg ttgctttaat accgctctcg gcacggccac tggttaatgg
     2041 agcagctgga ttttcaaatg ctgtatcact ttcgccataa taaacacggg cattaattcg
     2101 tggtgtgctg tgtaataact ctgcacggct agcttgccct aatgctttag tttcacgatc
     2161 tgattgagca acctcagcaa taagttttgt tgcttcagtc aattgtagtg tgagattagc
     2221 actggctaat tgatagttat cattaggaac atcttcttga acagccgccg cacctacagt
     2281 gattttttca cccaattttt gctcaacttg aacaccacca accacataat cttcacctgc
     2341 aacttcttca acttcaacag tcacacgaat tgcgtaaggg ttaagattgg catctaaact
     2401 agggactggc gatttaaaat aaatacttga tgttaaggct tcaaactcat aatcagtaaa
     2461 acgagtttta gcttcagtat taataattaa gcccaaatta ttgcggtcac gcttaataat
     2521 ttcgactttt tcactattta cttttaagcc atcaagacta ggcaaaacat aaggcccaga
     2581 aataccttgt gcaggaattt caaatactct ttgtttggca ttagtttgag caacaaaagt
     2641 cgttgcttta aaagtatctg ttgcgtaatg accacgaata cctgttaaag agcggttata
     2701 ttgtcctaaa gataaagcct ctgtaccctc aatacgtgtg gtgtaatccc caaacatgat
     2761 gtaagaacga cctttatcta agcgtacata taacttacta gtagattgtg catcaaaacc
     2821 acgtactgct gaatcaccat agacaggata gtaatcatct ggacgaatat cacggaataa
     2881 acgctgatct ttttctttat tagagtcata ggctaatgtt aataagtatt cccccttaac
     2941 tttgcctttt aagaacatcg cagcacgacc acctacattt aatttaccat cattgctact
     3001 tgatagattt tgtaattctg cttcaaaacc gtcattcgcg taagcaggtt gaactttggc
     3061 actatcaaag tttttaaagg tcaccatccc ttcaacaata cctgctgcca ccataggacg
     3121 taactcaggc acaaaacgaa tattttgttc tgtttttaac acaccactac tgacacgaat
     3181 tgttgattca cctgcttgtg ttggggcaac tagtttgaca ctaaattcac ccccctcaac
     3241 aaataattga atgccttgag ttttgggatc taaatctttg gctctaaaac gtcctgcatt
     3301 actttctaaa gtaatacttg tacgagtggt aattggcaca cccgcttcgt cttgtaattt
     3361 cactgacact aaaacttcat tgctaccatt tgcctcaacg actttttgat cagttgtcac
     3421 cagcaattta gccaagtttg caggtgcagt aaccaccaag ctacgagtgc cacgcacatt
     3481 acccaaacca tcaaattgtt ttaagacaat acggttttga cctgctttta agttaatacc
     3541 aatatattca cgcgcctcta gacgttgctc agtcactgcc atacgaatac ccatgctttt
     3601 ttcatcaagc acttgctcgt ttacacttaa ctcaaaacgt gcacctaaaa cacctttaaa
     3661 taaaatacga gtttgagagt aaggtaaaac ttgtccatca actaaattta aaatatctaa
     3721 ttgattagaa gaggtattgt ctaaatagct ttcaaggcta cgattatctt tggcaagctc
     3781 gatacgatta atatcatctt cttgagagtt ctgatctgct ttgactacag ccgttgttaa
     3841 cgtgggcatt ttggctttgc catgtaaacc acattcgtta cctgtataat taacacagcc
     3901 attagctggc aaagagcgta catcactggg attattacgt tcaaaagtca aatctgcttt
     3961 taattcgcgt tctaattccc ctatcgcctt atcaccttga ttacgacggg cataaatttc
     4021 tgccatcgct ggaccagaac aagtcgcatt atctgtggta atggcaaaat ctgctcggtg
     4081 aatttctccc gattttaagt caacaaaacg acttgatgga tcacctgcgt tacgaacatt
     4141 ttgctcaatt aactcaacat cttctggcaa agaagttcta tctaatttaa gaacatgtgt
     4201 tgttggacgt aagccataaa aattaaactt accttcaaca tccgtaacta cataagtacc
     4261 gtcttctaaa atcagacgaa cattaggcac cccgatttct tctaagcctt gtacaccatc
     4321 acgattacaa tcggtataaa ccttaccaat cacaaaggct tcttggctaa atacgcctgc
     4381 cgttactttc actgctgcaa cggcttcttg tgatgaagcc cctgtactat ctgttgcaat
     4441 aacacgattt ataccatcac catgtaatgc acctgtcccg atatgagctc tataagtcag
     4501 tttgacctct gcatctgctg ctagatctcc cactgtaaaa cttaacacag gcccaatacc
     4561 accttgagga ttttgctgca ctttgtctac acggccacta tcttgaacat aactaaagcc
     4621 tttaggtaaa gtatctttta ccaccacctg agtcgcaacc gtcttattaa cagacacatt
     4681 acgaacaaca acggtataat caacaaactc gcctaattca gcatttttac ggttactttc
     4741 ttttttgata aataaagaag ctttatcaat ggctggcgga tccattggga tatctaattc
     4801 tactggaccg tctgtgggta acacattaaa acggccgcca taagagccat catcatcacc
     4861 tgttgtaaaa tcacctgttg aatgaataac acgattcttg gtggctggat tattcaataa
     4921 tgctgttggt gataccacac tgggataggt ataaccatcg ggcgcagtaa tcaccaagcg
     4981 ataaaaactg cttatcgcat tggtggctgg cacaaacgga aatctaaaca taccgtcatc
     5041 acccgcatct acaacaccat caccattaat atctaaggtg cctgtaatga tcgttttttt
     5101 acaggatggg ctagcttctg tatcaacttc gcattcttta ccattatcat caaataactt
     5161 aaacggagct tctttgccat tttcaacacg aattaactta accgttgcac cattcagcaa
     5221 tttacctgtt ttgctatcaa acacgacacc aaaagggtca actaataact ctgtagaaac
     5281 ctcatctact tgattgattg ggtctttata aaccttcatt tctaagcctt gctcatcttt
     5341 tttgatggtt tttccatcat cgtctaagca gtgcataata gtagctctca cggtgtcgtg
     5401 agcaatggtt tctaaaatct cattttgttt aataggtgtg ttttctaaac gggaacgtgt
     5461 tggtaaaggt ttttctaagc gaaataaacc cgtgtcgttg cctgtttcta ttgctacaac
     5521 aaactcttca tcaaaaatac tgctatcttt accttggtta tctttaatta acgaacgcac
     5581 tctcaacaat aacttatctg ctactccaat tcgagtatta cattgctctg tttgcgtttc
     5641 taaaaataat ttttgattgg ccaaaatttt tcttcttaat gtagcgtagc ttgcattttt
     5701 actttctaaa ctagccaact caaaaatctg tatattggtt ttaacttgtt ctttagtggc
     5761 aaaatcttta taaattttca tctcttgatt ttgtaagtct tttttaatag ttttgccatc
     5821 atcttctaaa caatgaataa ttgtcacttc aactttgtca gttgttaaca cctctaaaat
     5881 ttgattttct tttgttacat taattaactg acgtgttttt tgatcacggc gagattgcgt
     5941 ttttaaagga ttagataagc gaaacaaact actatcagtt ttatcactgg cttcttcagc
     6001 tatcacaaac tcttcatcta taatggtggc tgaatcttct aaagcaaaag aacgtacacg
     6061 aagcaatact ttgtcggcaa cattcgcacg cgtattacat tgtgcaacac cagcctctag
     6121 ataaataccg tctgaatcga cagctttgac taaaactttt tgtttatcaa tcgtgtaatc
     6181 attatttttt gagcttaaac ttgcagaagc aacgcttact gtatctattg tattttttat
     6241 taagcccgag cgaacaccct tagtctgcaa cgcaatatta attttatcat ttaattttgc
     6301 atcagctggc gttgtaccaa tcacaaataa agtcacttgc tgttgtggat ttagaggaat
     6361 agcactactc acctcagtat ctacatcttt gtcataaaca ccattattat tactatcaat
     6421 aaatacctgc aaattggcaa tatccttgtt atcgtcagct aaattactta ctaaaatatc
     6481 ataactatct gctaatgaac ctgtattttt taggatgtgt ttccaaataa caggttgatt
     6541 agctacaacc gaaggaggat tctgttgact atttgcatct gttaacgcat ctgttaagct
     6601 catatctaag cccgaaccta agctagaatt aataattaac tcatcattca cggattgctg
     6661 ttgattagta tttttgcttt gagcggttag tgttaatttt attttatctt gatttgctag
     6721 atcgctgggt acagtaccaa caagcaataa tttggcactt ttatttgcat ctagtctcaa
     6781 ttcggtaatt tgctgatcta tgccaacatc atatcgctca ttattgttta tatcttgaac
     6841 gataatcaca tttaataaat cactattatc attagttaat tgagtgactg ataacgtata
     6901 gctttctgca aaactaccgt tattttgcaa cacatgaggc aatgcaacga catcattcag
     6961 ttttgctaca cggctattat caatcaataa actgaaagag tttttttgca ataatgtggt
     7021 taccacatta gaaaaagctt gtttttcttc attgccatat ttgtaataac tgacaaactg
     7081 attatttaag tttgagagct gctgtgttgt tacttcaact tctacatcaa tttggccttc
     7141 ttgtttaaca tcaaatgatg aataagcaac cgcaatacga tcaatttgtg ttaatgatgg
     7201 taacgtacca ctgttataac gatgataaac ttgttgagac tcacctttta gatgataaag
     7261 cagctcccct ccccctttac gcagataaat atctttaaaa gtgacaccta caggcaatgg
     7321 gtcatcaaaa acaacttttt gctgattaac accatcaata ctcaccgtag tacctaaggc
     7381 aactgcatta cctgtgttac taaactcaag gctataaagt aaggtattac ttgggttatt
     7441 gagatttatc tgggttttgt taacattttt acttaaaaca aattgtgggg cattggtata
     7501 aacattatct gtttgttgca gtactggcac accttgagaa ccaaagactt tgagcgatac
     7561 gctaaactga ctgcctggca acaaagttgc tggcaattgc gctctaacca acaaagacat
     7621 actttgtcca accgcaatct gctctaaatg tgcttctgga tcaataatga catcaccagc
     7681 atcaaacgca ccatttttat taacatcatg aattagttga ggctttatta aaataccgct
     7741 atcaccaccc acatcggtca aactaaaacg ataaccatca tcagcattac ctgtgttggt
     7801 taaaatatgt ggccaaaata cgacctgact tggagaagca acgatatcat tagctggtgt
     7861 tagagttgca gctaaaacag gcaatacttg aacaataaca ggctgcgatt ttaatagata
     7921 agtttcgcct gtttcattga caaactgggc aaaagcttca ttttttattt gatagcctgc
     7981 agcaggcatt tgcgcataag ccactccaca aagcatcata cataacgcaa ctaacgtgcg
     8041 taacatataa atgacttgtg tagaatagtt taaaaaatac acgatatagc ctttattttt
     8101 agaagaatta tgtaattgat tggcacaagt tgataaacac atacgttgta cctaacttat
     8161 atgacttagg ctataaacag cacataagca caacagagga ctaagcctaa aagagtcagt
     8221 aaaacctgac tcttttagca aatgagcgtc cctgctctgt attaaaaatt aatcaattaa
     8281 ggattattga tcaattttaa cact
//
LOCUS       DAOQMB010000059         8255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3481_length_8255_cov_15.191707, whole genome shotgun sequence.
ACCESSION   DAOQMB010000059 DAOQMB010000000
VERSION     DAOQMB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_8255_cov_15.191707"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLY05_03565"
     CDS             complement(<1..422)
                     /locus_tag="PLY05_03565"
                     /inference="COORDINATES: protein motif:HMM:NF017386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HNC02413.1"
                     /translation="MTTLYELSDKILTALDIFSEMDECSEAEKAEAQVIIGQLTESFN
                     DKAVSVAMFIRNIEVEAEAIAEAKKSMDAREKALKAKAARLKNYLLSEMKRTDTKQIK
                     CPYFVVSVRKNPVSVVISPNAVIDTDLLLPPKPQEPDK"
     gene            complement(468..659)
                     /locus_tag="PLY05_03570"
     CDS             complement(468..659)
                     /locus_tag="PLY05_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02414.1"
                     /translation="MNHKAEYEAIKKQIAECEQELKNRCIECSNFNKKKGECLKHGFV
                     PVEFIYQKNDCLDYDLLPF"
     gene            complement(656..2218)
                     /locus_tag="PLY05_03575"
     CDS             complement(656..2218)
                     /locus_tag="PLY05_03575"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02415.1"
                     /translation="MITLRDYQQDAVQSAYAYWHNGTSCIIEAPCGAGKSLIIGKICH
                     DSITHDVRVLVVTHRKKLLEQNEAELKNLLPDADTGFYSAGLNQKTQDAQIVFAGIQS
                     IANAKIQHYEILIIDECHLVAPNESGQYHALISKLKEVNPDLKILGLTATPYRLDSGY
                     LTEWETPIFERVVYKIDVKLLIKRGYLCPVVSNGGGVKVDVSKVKHKGGEFLDSALES
                     LYMSKTVEIVADIVKKGIDRKAWLIFCVSIEHAEQVTTELISHGIDAACYHSQSDNEY
                     ILDDFANGRLKCLVNVNILTTGSNFPIADMCVLLRATESTALYVQIVGRVMRLYPNKK
                     NALLLDYGGNVLRHGCIDDVAVKAKGEGTGEAPAKECPSCETILHAAVRECPECGYIF
                     EREPDKKLELNAFDGAVLSDQRKVQKVPVDRVSFKIHKKIGKPDSIKVTYHCGMAEYY
                     EWLTPEHSEFGLKKTHAFFRNIDDDYFESSSVSIEFLEDLNAIRHSKKITSIDILPSK
                     YTEVKKRYWSKV"
     gene            complement(2270..>2605)
                     /locus_tag="PLY05_03580"
     CDS             complement(2270..>2605)
                     /locus_tag="PLY05_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02416.1"
                     /translation="MLVIMTAHSHVNKVEDPEHLTFDQHDLKLHKKAAALCREFADVI
                     GYASLKKIIKITEGKGFNDDRNRAITTGERILNLSASPAYVAKNRYDMASQMPLLWSE
                     FAKSLPSQK"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2929..3408)
                     /locus_tag="PLY05_03585"
     CDS             complement(2929..3408)
                     /locus_tag="PLY05_03585"
                     /inference="COORDINATES: protein motif:HMM:NF016896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HNC02417.1"
                     /translation="MSNLSGYNFNAEEIEPSSFDPIPAGWYKAIISASEMKPTRDGYG
                     EYLSLTLQVIEGQYANRLVFARLNLKNANAKAVDIARKDLAAICRAVGVMSPQSSEEL
                     HDKPLMIKVKVRPAQGEYDASNDVGGYKADEGTDFTPPPPPKAPTPAATPAKKPWQK"
     gene            3542..3724
                     /locus_tag="PLY05_03590"
     CDS             3542..3724
                     /locus_tag="PLY05_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC02418.1"
                     /translation="MLSLPEIKKLLEDRQLNVVAERVGIHSNTIYRLVKFERAEYDTI
                     KKLSDYLEGQLENAKK"
     gene            3711..6011
                     /locus_tag="PLY05_03595"
     CDS             3711..6011
                     /locus_tag="PLY05_03595"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HNC02419.1"
                     /translation="MQKSKEAASSYVNHGFKLCLVRGKKPFQDKWEQNPVTDLNLFDH
                     NGIGLIHGLSGTCTLDIDNIEQSTIALAACGVDLTALMAEGVRIESGRENRSKLLFKA
                     PVGVELKRHALNWTKEDNPKESDVVFELRGGFTQDVLPPSIHPDTGQPYFWVGDYKNI
                     PELPHELLNIWTQWDIAKDVLKSACPWHIEKEDYKAQSAPVRVFSSDNDVIGTFNKRM
                     PLASILGNYGYKRITKTRLLSPHSKSKLAGCILLTGGDVDKVYIHHASDPLGDGYAHT
                     AFGVYLYYQHNNDLKAAVKEAALLLDMDYKKPPEDETLLQQGNEIANTFLNANVVKLK
                     PVQVDTVKIDCKLPVESLNEVAAWIKGQIGTAPKYSIVQATLSFACAMASRCVRLKDG
                     TASSAFLAIVADSAGQIQPLKGILNSAIDACGDRQIIRGTKISSSTCLHKQLLTMPRM
                     FWATDDYATMINFGKKQQSGAIQGALSAINEVYLNNTLYLDKDSIGASFGKKDGDGDK
                     HISEYNIYRPSLTMLSLMSYKHIDFVAQRDQYSIGSLQRLMIADGGDSVTCERDFDAP
                     FPTNVKTVVAAIKKTGSEFIDIASMNPVQKIAIFDCDNTATLFTHSLNRIKATCSSDE
                     RKDLLGIALGWCGSFKRLCVALGAFNKPNNPTINEAIVQWCSNWIVFHLEKLLSRLEI
                     NGLDEEIGIEEEVLNVVYDFGKKGASSRDIGRKHRAFRNLDAVQKLEMLAKLEAQEKI
                     IEKKEGKAVRYFMPVFFKSDCTTNAK"
     gene            6526..7815
                     /locus_tag="PLY05_03600"
     CDS             6526..7815
                     /locus_tag="PLY05_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02420.1"
                     /translation="MKLTPKQQTAFDTIIECINSNTPVLLTGFAGTGKSTTIATVIKS
                     LSHKLITIATPTHKASAVLSAMLETNGIVSPNVKVTTIHKALGKRPQRQGGGTMTFSK
                     PTKDIYGILIIDECSMIDAELFDDINNAASFASIVYVGDPAQLPPASGNGALSPVFSS
                     IAHRAHLSEVIRQGDGNPIIELSAALRRVMESASHITVSDVIEMVNEYDKDNEKIGMI
                     QKGDIAEYCTDAINNGLDCRYLAYRNDTIDKHMKEIRQQLHGRDVQGFVVGEPVTSLT
                     SIQGVINNNHEGNITHIGEVTKLHGITCTPVTLDNELTVFAAIDIKEKQNKEIGFFRL
                     FEKLKAQAGLTTDFKAKADALEKSQEASAKGYKIRDEIAELRPCVASTVHKAQGSTFD
                     VAIVDVADILTMRNRSEALQCLYVAVTRPRSYLILVV"
     gene            7817..>8255
                     /locus_tag="PLY05_03605"
     CDS             7817..>8255
                     /locus_tag="PLY05_03605"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNC02421.1"
                     /translation="MSLKSLIDKTLSKQPLIKPSRTKKRNAQPEAIEQERVIKWARDN
                     ENNYPFLWLLHSSLNGVKLSKNQAGRAKTQGMLSGVPDLFLPVPKKGFHGLFIEMKSQ
                     QGRVSVSQSRFLSVTNELGYQSIVCYSSEDAIEKIKEYLKNVTH"
BASE COUNT         2408 a         1599 c         1743 g         2405 t
ORIGIN      
        1 tttttgtcgg gttcttgtgg ttttggtggt aacagtaaat cagtatcaat aacagcattt
       61 ggtgagataa caacagatac gggattttta cgcacagata caacaaaata agggcatttt
      121 atttgttttg tgtcggtacg tttcatctcg ctcaataggt aattttttag tcttgccgct
      181 tttgctttta gtgctttttc acgcgcatcc attgattttt ttgcctctgc aatcgcttct
      241 gcttcaactt caatgttgcg aataaacata gcaacagaaa cagctttgtc gttaaatgac
      301 tcggttagtt gaccgataat gacctgtgct tctgcttttt cagcttcgga gcattcgtcc
      361 atctctgaaa aaatatctaa tgcggtcagt attttgtcgg ataattcgta gagtgttgtc
      421 ataatggact ctctttcggt tggtttagtt tgtaatatat tgacttgcta gaacggcagc
      481 aaatcatagt ctaaacaatc atttttctga taaataaact caactggcac aaaaccatgc
      541 tttaaacact cacccttctt tttgttaaag tttgagcatt caatacaacg attttttaat
      601 tcttgctcac attctgcaat ctgttttttg attgcttcat actctgcttt atggttcata
      661 ctttactcca gtaccgtttt ttaacttcgg tgtattttga tggtagaatg tcgattgatg
      721 ttattttttt agaatgacgt atagcattca aatcttctaa aaactcaatt gatacactag
      781 aagattcaaa ataatcatca tctatatttc taaaaaaagc atgagtcttt tttaatccaa
      841 actcgctatg ttcaggcgtt agccattcgt aatactctgc catgccgcaa tgataagtca
      901 ctttgatgct gtcaggtttt cctatcttct tatgtatctt aaaactcacg cggtcaactg
      961 gtactttttg tactttgcgt tggtctgata gcaccgcacc gtcaaaggca tttagctcta
     1021 gttttttgtc aggctcacgc tcaaatatat atccacattc tggacactca cgcacagcag
     1081 catgaaggat tgtttcacat gatgggcatt ctttagcggg tgcttcgcct gtgccttcgc
     1141 ctttggcttt gactgccaca tcatcaatac agccgtggcg tagtacgttg ccaccataat
     1201 caagcaatag tgcgtttttt ttgtttgggt acaacctcat aacacggcca acaatctgca
     1261 cataaagcgc ggttgactcg gtagcacgaa gaagaacaca catatccgca atagggaagt
     1321 ttgagccagt cgttaagatg tttacattaa caaggcactt taggcggcca tttgcgaagt
     1381 catctaaaat atattcatta tcgctttgtg agtgataaca agccgcgtct atgccgtgac
     1441 ttattaactc ggtagtgact tgttcagcgt gttctattga tacgcaaaag attagccatg
     1501 ctttacggtc aatgcctttt ttgacaatat cagcgactat ctctacagtt ttactcatgt
     1561 ataacgattc aagcgcactg tctaaaaatt caccgccttt gtgtttaacc ttgctcacat
     1621 ctactttaac accgccaccg tttgacacta cagggcatag atagccgcgt ttaatgagca
     1681 atttaacgtc aattttatac acgacacgct caaaaatagg cgtttcccac tctgtcaaat
     1741 aaccactgtc taagcggtac ggggtggcgg taaggccaag tatttttaaa tcaggattaa
     1801 cttcttttag cttgcttatt agtgcgtgat actgtcccga ctcattgggt gcaacaagat
     1861 gacattcatc aatgataagt atttcatagt gttgtatttt tgcattggcg atgctctgaa
     1921 tgcctgcaaa aacaatttga gcgtcttgtg ttttttggtt taatcctgca ctataaaaac
     1981 ccgtatcggc atcaggcaat aagtttttta attcagcctc attttgttct aatagttttt
     2041 tgcgatgcgt gacaaccaaa acgcgcacgt catgggtaat tgaatcatgg catatcttgc
     2101 caataattaa acttttgcct gctccgcatg gtgcttcaat aatgcagctt gtgccgttat
     2161 gccagtacgc ataggcactt tgtacagcgt cctgttgata atctctaagt gtaatcatag
     2221 ttggcttctc ggtttgtgcg gtacgcaatg accgcacttt aggggtgtgt tatttttggc
     2281 ttggtagtga ctttgcaaac tctgaccaaa gcaacggcat ttgagaagcc atatcgtaac
     2341 ggtttttggc aacataggca ggacttgcac ttaggttaag gattcgctca cctgtggtta
     2401 ttgccctgtt tctgtcatca ttaaagcctt ttccttcggt gattttgata atctttttta
     2461 acgaggcata gccaataaca tcagcaaact cacgacacaa agcagccgct tttttgtgta
     2521 gtttgaggtc gtgttggtca aaggttaggt gttcggggtc ttctactttg tttacgtggc
     2581 tatgggctgt cataatcaca agcatnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnggcaa gggggaagca tggcacatca atttcgccta gtccgtcctc tgtttgaatc
     2761 acaatagggt taggtgcaga cgtagcaaat gttgttttgc ctaatccact ctcgccgtaa
     2821 ataattacac gctctgtttt tggggtgttg cgtgtgatgc tatttaaaaa tgacataatt
     2881 actactccat aaattaaaaa agccgccaca atgggcggct atttgttgtt atttttgcca
     2941 aggttttttg gcaggtgtgg cagcaggtgt aggtgctttt ggtggcggtg gtggtgtaaa
     3001 gtctgtacct tcatcggctt tgtaaccacc tacgtcattg cttgcgtcat actcaccttg
     3061 tgcagggcgt actttaactt taatcatcaa aggtttgtca tgcagttctt cgctagattg
     3121 aggagacata acgcccacag cgcggcaaat ggcagctaag tctttacgcg caatatcaac
     3181 agccttggca tttgcgtttt ttaaattcaa acgtgcaaac actaaacgat tagcatattg
     3241 accctcaatt acttgtaaag ttaaagaaag atactcaccg tagccatcac gtgttggttt
     3301 catttcagac gcgctaataa tagctttgta ccagccagca gggattgggt cgaatgatga
     3361 cggttcgatt tcttcggcat tgaagttgta gcctgataaa ttgctcataa tatactcact
     3421 gtttcgttag tttaagattc actggtttcg tttgtttcgc gtgccagtgg tgccagtggt
     3481 tgataagata actataattt gttattatgt caacactcac aaacaaaaaa gaggttaaat
     3541 tatgttatcg ctgcctgaaa ttaaaaaact gttagaagat agacagctta atgttgtcgc
     3601 cgagcgtgtg ggtattcact caaatacaat ttatcggttg gtaaagtttg aacgtgccga
     3661 atatgacaca attaaaaaac tctctgacta cttagaagga caattagaaa atgcaaaaaa
     3721 gtaaagaagc cgcatcaagt tatgttaatc atggttttaa attgtgccta gtgcgtggaa
     3781 aaaagccatt tcaagataaa tgggaacaaa accctgttac tgatttaaac ctgtttgacc
     3841 ataacggcat tggtttgatt catggtttga gtggtacttg tacgctagac atcgacaaca
     3901 ttgagcaatc tactattgcg ttggctgctt gtggtgttga tttgactgct ttgatggctg
     3961 agggtgtacg aatcgaatca gggcgtgaga atcgctctaa attgttgttt aaagcacctg
     4021 tgggcgtaga attaaaacgc catgcgctaa actggactaa agaagataat cctaaagagt
     4081 cggacgtggt gttcgagttg cgcggtggtt ttactcaaga cgtattgccg ccctctattc
     4141 atcccgacac tggacagcct tatttttggg tgggtgacta taaaaatata cctgaattac
     4201 cccatgaatt attaaacatt tggacgcaat gggatattgc taaagacgta ttaaaaagcg
     4261 cgtgtccgtg gcacattgag aaagaggatt acaaggcgca atccgcacct gtgcgcgtgt
     4321 ttagtagtga taatgacgtg ataggtacgt ttaataaaag aatgccccta gcgagcattt
     4381 taggcaatta tggctataaa cgtatcacca aaactcggct attgagtcca cactctaaaa
     4441 gcaaattagc aggttgtatt ttgttgactg gtggtgatgt tgataaggtt tacattcatc
     4501 acgcaagcga cccactaggc gatggctatg cacatacggc ttttggtgtg tatttgtatt
     4561 atcagcacaa taacgattta aaagcggcag ttaaagaggc cgcgttattg ttagacatgg
     4621 attacaaaaa accacccgaa gacgaaacgc tattacaaca aggcaatgag attgccaata
     4681 catttttaaa tgccaatgtt gtcaagttaa agcctgtgca agttgacact gtaaagattg
     4741 attgcaagtt accagtggaa tcattaaacg aggtggcggc atggattaag gggcaaatag
     4801 gtacagcacc gaaatactct attgtccaag caacattatc atttgcttgt gctatggcta
     4861 gccgttgtgt gcgtttaaaa gacggtacag cctcaagcgc gtttttagcg attgttgcag
     4921 actcagcagg gcaaattcag ccactcaagg gtattttaaa cagcgcgatt gatgcgtgtg
     4981 gtgataggca gattattcgt ggtactaaaa tcagtagttc tacttgtttg cataaacagt
     5041 tgctaactat gccgcgtatg ttttgggcga cagatgatta cgccacaatg attaactttg
     5101 gtaaaaaaca gcaatctggc gcaatacagg gcgcattgag cgcaatcaat gaggtttatc
     5161 tcaacaacac actgtattta gataaagaca gcataggcgc gagttttggc aaaaaagacg
     5221 gtgatggtga taagcacatc tctgaatata atatctatcg tccatcttta acgatgctca
     5281 gtttaatgag ttacaaacac attgattttg tcgcacagcg tgaccaatac agcatcggta
     5341 gcctacagcg tttaatgatt gccgatggtg gcgatagcgt aacctgtgag cgcgattttg
     5401 acgcaccatt cccgacaaat gttaaaacag tggtcgctgc aatcaaaaaa acagggagcg
     5461 aatttattga tattgcttca atgaatcccg ttcaaaaaat tgctattttt gattgcgaca
     5521 ataccgccac cttgtttaca cactcgctaa accgtattaa agccacttgt agcagtgacg
     5581 agcgaaaaga cttgttaggt attgctttgg gttggtgtgg tagttttaag cgtctatgcg
     5641 tggctttagg ggcgtttaat aaaccaaaca atccaacgat taacgaggca attgtgcaat
     5701 ggtgtagcaa ttggattgtg tttcatttag aaaaattact ctctcgttta gagataaatg
     5761 gacttgatga ggagataggg attgaggagg aagttttaaa tgttgtgtat gactttggga
     5821 aaaaaggtgc atcaagccgc gacattggac gcaagcatcg agcgtttaga aacttagacg
     5881 cagtacaaaa attagagatg cttgcgaaac tagaagcaca agaaaaaatc attgaaaaaa
     5941 aggaaggcaa agcggttcgt tattttatgc ctgttttttt taaaagtgat tgcacaacaa
     6001 acgcaaaata agttaatgtt gaggggttgg aatgatttta gccccttgtt tttgctcctt
     6061 gtgacgtttc tatagtttgt gacaccactg ggacacgagc aaaccttaca gccgcaaggg
     6121 ctgcaagaga aacgtcccaa cgtcccagcg tcccacttca aaaacaattt tagaaaaaat
     6181 tgtcgtcttt taacaaaatc ccataaaaac cttaatacaa tgctctctct ctgtaagtat
     6241 tatatatatt attatatacg cgggacgtta cgttgggacg attccactct agccttagag
     6301 ccgcaagggc tacagccgtc ccacgaatcg gcacacgcaa aaaaagaggg acactttaaa
     6361 aaaactataa aaaaatgctt ttttatagct aaaagttatt agaaaaaaca cgctaaaaac
     6421 gcgataatta caaagcggct agggtagctc ccgaaaagtg agagtcattc actcacagcc
     6481 gcctcccccc ccccgaatga ttgccactaa tgaaggctca acaatatgaa actcacaccc
     6541 aagcaacaaa cagcatttga cacaatcatt gagtgcatca attcaaacac accagtttta
     6601 ttaacgggct ttgcaggtac aggcaaaagt acaacaatag caacagttat caagtcgcta
     6661 agtcataaac ttattaccat tgctacgcca acacataaag catcggcggt attgtctgca
     6721 atgcttgaga caaacggcat tgtttcgcca aacgtgaaag taaccacaat ccacaaagca
     6781 ctcggcaagc gtccacagcg tcaaggtggc ggtacaatga cgtttagtaa gccaacaaaa
     6841 gatatttacg gtatattgat tattgatgaa tgctcaatga ttgatgctga gttattcgat
     6901 gatattaaca atgccgccag ttttgcctct atcgtttatg tgggcgaccc tgcacagtta
     6961 ccacccgcaa gcggtaacgg ggcattaagt cctgtatttt cgtcaattgc acatagagcg
     7021 catttaagcg aggttattag acagggcgat ggtaatccca ttattgagtt atcagcagca
     7081 ttaaggcgtg ttatggaatc agcaagccat attaccgtaa gcgatgttat tgaaatggtg
     7141 aacgagtacg acaaggacaa cgaaaaaatc ggaatgattc aaaaaggcga cattgccgag
     7201 tattgtactg atgcaataaa taatggttta gattgccgat atttagctta ccgtaatgac
     7261 acaatcgaca agcacatgaa agagattaga cagcagctac acgggcgcga tgtgcaagga
     7321 tttgttgttg gtgagcctgt cacgtcatta acaagcatac aaggcgtaat caataacaat
     7381 cacgagggca atataaccca cattggcgaa gtcacaaaac tacacggcat cacctgcaca
     7441 cctgttacat tagataacga attgacagtt tttgccgcga ttgacatcaa agaaaagcaa
     7501 aataaagaaa taggcttttt tagactgttt gagaaactca aggcacaagc aggactaacg
     7561 acagatttta aagctaaagc agacgcactt gaaaagtcac aagaagcaag cgcgaaaggt
     7621 tacaaaatac gggacgaaat agccgagctt aggccatgtg tagcgtcaac agtacacaag
     7681 gcacagggaa gcacatttga tgttgccatt gttgatgttg ccgacatact cacaatgcgt
     7741 aaccgtagcg aggcgttaca gtgcttgtat gttgccgtta ctcgaccccg cagttattta
     7801 attttggtcg tgtaatatga gtttaaaatc attgattgat aagacactat ctaagcaacc
     7861 actaatcaaa ccatcacgca ccaaaaaacg caatgcacag cccgaagcaa tcgagcaaga
     7921 gcgcgttatc aagtgggcgc gtgataatga aaataattac ccgtttttgt ggttgctgca
     7981 tagcagttta aacggtgtca agttatcaaa aaatcaggca gggagggcaa aaacacaggg
     8041 aatgttaagc ggtgttcctg atttgttttt gcctgtcccc aaaaaaggtt ttcacggatt
     8101 atttatcgag atgaaaagtc agcaaggccg agtatctgtt agtcaaagtc gttttttatc
     8161 tgttacgaat gaattgggtt atcaatctat tgtgtgttat tcgagcgaag acgctatcga
     8221 aaaaatcaag gaatatttaa aaaatgttac tcatg
//
LOCUS       DAOQMB010000060         8196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3518_length_8196_cov_16.777177, whole genome shotgun sequence.
ACCESSION   DAOQMB010000060 DAOQMB010000000
VERSION     DAOQMB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3518_length_8196_cov_16.777177"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..3719)
                     /gene="metH"
                     /locus_tag="PLY05_03610"
     CDS             complement(3..3719)
                     /gene="metH"
                     /locus_tag="PLY05_03610"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HNC02422.1"
                     /translation="MTASRSDLLKDALKQRILIIDGAMGTMIQRYKLQEADYRGSRFA
                     DWPHDVKGNNDLLVLTKPNVIAEIHDAYCAAGADILETNSFNSTQVSQADYHMEALVY
                     ELNVEAARLAKQVALAWSAKTPEKPRFVAGVLGPTSRTCSISPDVNNPAYRNVTFDEL
                     VENYVEATKGLVDGGADILLIETVFDTLNCKAALFAVQKYFDDTGTILPVMISGTITD
                     ASGRTLSGQTAEAFWNSVRHGDLLSIGFNCALGADAMRPYIKELSEKADIFVSAHPNA
                     GLPNAFGEYDETPAETAAMVGEFAQSGLVNIVGGCCGTTPDHISAIYNAVKDLAPRVI
                     PEIEPACRLSGLEAYNITKDSLFVNVGERTNVTGSKKFARLIREQNYAEALDVARQQV
                     ESGAQIIDINMDEGMLDSQAAMVTFLNLIASEPDISRVPIMIDSSKWEIIEAGLKCVQ
                     GKPIVNSISLKEGHEEFVQKAKLCRRYGAAIIVMAFDEQGQADTAARKREICERSYRV
                     LVDEVGFPAEDIIFDPNIFAVATGIEEHNNYAVDFIEATGWIKQNLPHAMISGGVSNV
                     SFSFRGNEPVREAIHAVFLYHCIKQGMTMGIVNAGQLAIYDDIPAELKDAVEDVILNR
                     NQGESGNEATEKLLAIADKYREHGKTNDNAAPETLEWRNQSVEKRLEYALVKGITTFI
                     NEDTEEARLQSKRPLDVIEGPLMAGMNVVGDLFGAGKMFLPQVVKSARVMKQAVAWLN
                     PYIEAEKVVGQSKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIVDLGVMVPCEKILD
                     VAIKEKVDIIGLSGLITPSLDEMVYVAREMQRQGFNIPLMIGGATTSKAHTAVKIDPQ
                     YHNDGVIYVTDASRAVGVATSLLSPEMKPVFVAERRAEYAEVRERHANRQPKAAKLSY
                     AQSIAEKYQHDWANYTPPKPKQLGVTVLKDVPLADVRPYIDWTPFFITWSLAGKFPAI
                     LKDEVVGVEATRLYNDAQALLDKLIGEKLLTANGVIGFWPAAQVNDDDIEVYADESRQ
                     TVIHTLHHLRQQSDKVSGKPNYSLADFIAPKASQIPDYIGAFAVTAGLGAEEQARAYR
                     DAGDDYNAIMVQALADRLAEAFAEYLHERVRKQYWGYQADESLSNEELIKEKYVGIRP
                     APGYPACPEHTEKATLFKLLDAEVNAGMSLTEHFAMWPAASVSGFYYSLPQSTYFNVG
                     KLDKDQVESYAARKGWDLRMAEKWLSPNLGYEPE"
     gene            3868..4473
                     /gene="nfuA"
                     /locus_tag="PLY05_03615"
     CDS             3868..4473
                     /gene="nfuA"
                     /locus_tag="PLY05_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HNC02423.1"
                     /translation="MSNLNINITESAQAYLRELLAKQNSEGIGVRIFVENAGTPKAEC
                     CMAYCTQGEEQIDDIRLPMEGFPAYIEFKSSPYLEDAVIDYNKDRFGGQLTFKAPKSK
                     VPLLGENSSLEERINHVLFSEINPSLAAHNGNVSLLDVYEDTEHGLTAVLKFGGGCQG
                     CSAVDMTLKQGVETTLKANIPELQRVTDSTDHSQRDNAYFK"
     gene            complement(4504..4743)
                     /locus_tag="PLY05_03620"
     CDS             complement(4504..4743)
                     /locus_tag="PLY05_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02424.1"
                     /translation="MAIFRMILGIFLVLMAPAMIYIGLDAEKHGIDLGELNGTRGAAM
                     FDLFGMWGMALIFFGFGVSEILTGKKLLKEKDKRN"
     gene            complement(4835..5719)
                     /locus_tag="PLY05_03625"
     CDS             complement(4835..5719)
                     /locus_tag="PLY05_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC02425.1"
                     /translation="MPDFYFILAGLVVGFCVGVTGVGGGSLMTPILISFLRVEPHIAI
                     GTDLLYAAISKFCGSLVHAKKLNIVWPIVFWLGLGSIPASLATTWALEHFMGGAKEYK
                     KLLTIILGGMLVLTGISIVFRQKIEAFFNRHQHDVAGEALTADEHSQVVLKHKGWVVL
                     MGIFLGFFVTLSSVGAGAFGIMALVLLFPKLPMIRIIGSDVAHAVLLTLVAGLGHLKA
                     GNVDTHLLMLLLMGSIPAIIVGTLLSSRLPERAIRKVLGVTLILLGTNFIVNPVKVKP
                     KPVTPPPVTMVAPLRQAV"
     gene            5837..6811
                     /gene="cysB"
                     /locus_tag="PLY05_03630"
     CDS             5837..6811
                     /gene="cysB"
                     /locus_tag="PLY05_03630"
                     /inference="COORDINATES: protein motif:HMM:NF009326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HNC02426.1"
                     /translation="MKLQQLRYICEVASHELNISTAAQNLFTSQPGVSKQIRLLEDEL
                     GVEIFTRSGKHLTQITPAGQEVLKIAHEILRQVENIRQVGEEYSNPYMGRLAIATTHT
                     QARYRLPPVIEQFRKRYPEVSLHLQQGTPQQIAQMASSGLVDFAIATEAMEHFNNLIL
                     LPCYRWNRSVVVPKNHPLTKLPQLSLEAIAQYPIVTYVFGFTGRSQLDDAFSQHGLTP
                     KVVFTATDADVIKTYVKLGMGVGIIAHMAYEPHIDTDLVALDVSHLFASSTTKIAFRK
                     GMWLRSFMFDFMAAFAPHLTRARVEAAILAETTEEREQIFAKVELPVL"
     gene            complement(6871..7206)
                     /locus_tag="PLY05_03635"
     CDS             complement(6871..7206)
                     /locus_tag="PLY05_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02427.1"
                     /translation="MHITMVKKILADGSPCKKCGEVLAKMEADDQLKFMNEILVADER
                     DPESAGMQLAKKLGVDRAPFFVVSQDDGSQTIYTVYLKFVKEVLNATTNEKEELKELM
                     NNSQDLDFL"
     gene            complement(7339..8064)
                     /locus_tag="PLY05_03640"
     CDS             complement(7339..8064)
                     /locus_tag="PLY05_03640"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HNC02428.1"
                     /translation="MTITHANAQQWATDYEEKTPQEILALALENFPDVAISFSGAEDV
                     VLIDMAAKLGKPFRVFSLDTGRLHAETYQFIEKVRNHYKVNIEICFPESAQVQEMVNA
                     KGLFSFYADDHKECCTVRKVAPLKKKLATLDAWITGQRKDQSPGTRLAVPVVQADAAF
                     SGAGKQLIKFNPLSNWSSAQVWDYIRSNEVPYNALHEKGFTSIGCEPCTRAILPNQHE
                     REGRWWWEEATKKECGLHAVNIK"
BASE COUNT         2463 a         1805 c         1583 g         2345 t
ORIGIN      
        1 acttactcag gctcataccc caaattaggg cttaaccatt tttcggccat acgcaaatcc
       61 caacctttac gcgcagcata actttctact tggtctttat ccaatttacc gacattaaaa
      121 taggtacttt gtggtaaaga ataataaaaa ccactcaccg acgcggcagg ccacatggca
      181 aagtgttcgg ttaaactcat gcccgcattg acttcagcat ctaataactt aaataacgtg
      241 gctttttcgg tatgttctgg acaggcagga taaccaggtg caggacgaat accgacatat
      301 ttttctttaa ttaactcttc attgctgagg ctttcatcgg cttgataacc ccaatattgc
      361 ttacgcactc gctcatgtaa atattccgca aaggcttcgg ctaaacggtc agcaagggct
      421 tgtaccataa tcgcattata gtcatcaccc gcatctctgt aggctcttgc ttgttcttca
      481 gcacccaaac cagcagtcac tgcaaatgca ccaatataat caggtatttg gctcgcttta
      541 ggcgcaataa aatcggctaa agaataattg ggtttgccac tgactttgtc actttgttga
      601 cgtaaatgat gcaatgtgtg aatcactgtt tggcgtgatt catcggcata tacctcaata
      661 tcatcatcat tgacctgtgc ggcaggccaa aaaccaatta caccattggc ggttaatagt
      721 ttttcaccaa ttaatttatc taacaaggct tgggcatcgt tgtataaacg agttgcttca
      781 acaccaacta cttcatcttt taaaatggca gggaacttac cagccaaact ccatgtgatg
      841 aaaaaaggtg tccagtcaat ataaggacgt acatcggcta aaggtacatc ttttaacacg
      901 gtcacaccca attgtttggg tttgggtggt gtgtagttag cccaatcgtg ttgatatttt
      961 tcggcaatag attgtgcgta actgagttta gcggctttag gttgacggtt ggcatgacgc
     1021 tcacgcactt cagcatattc ggcacgacgt tcagcaacaa aaacaggttt catttctggc
     1081 gagagtaggg aagtcgccac acccacagcg cgtgaagcgt cagtcacata aatcacgcca
     1141 tcattatgat attgcgggtc aatttttacc gcagtatggg ctttagaggt agttgcaccg
     1201 ccaatcatta atggaatgtt aaagccttga cgctgcattt cacgcgcaac atacaccatc
     1261 tcgtctaagc taggggtaat caaacccgac aagccaataa tatcgacttt ttctttaatg
     1321 gcgacatcaa ggattttttc gcaaggcacc atcaccccta aatcaacaat gtcataacca
     1381 ttacaaccca agaccacacc aacaatattt ttgccaatat cgtgaacatc gcctttaacc
     1441 gtggccatta agattttacc tttggattgg ccaacgactt tttcggcttc aatgtagggg
     1501 ttgagccatg ccactgcttg tttcatcacc cgcgccgatt tcaccacttg tggcaaaaac
     1561 attttacctg cgccaaacaa atcacccacc acattcatac cagccattaa aggcccttca
     1621 atcacgtcta aggggcgttt gctttgtaaa cgggcttctt cggtgtcttc gttaataaac
     1681 gtggtaatac cttttaccag tgcatattcc aagcgttttt caaccgattg attgcgccac
     1741 tcaagggttt caggtgcagc attgtcgttg gttttgccat gttcacggta tttatcagca
     1801 atggctaaca gtttttcggt ggcttcatta ccgctttcac cttgattgcg gttaagaatc
     1861 acgtcttcaa cggcatcttt taactcagca ggaatatcgt cataaatcgc gagttgtccc
     1921 gcgttaacaa tacccatggt catgccttgt ttaatacagt gatataaaaa cactgcatga
     1981 atcgcttcgc ggactggttc gttaccacgg aatgagaacg acacgttaga aacaccaccc
     2041 gaaatcatgg catggggtag gttttgttta atccaaccag tcgcttcaat aaagtccacc
     2101 gcatagttgt tgtgttcttc aatacctgtt gcgacggcaa aaatgttcgg gtcaaaaata
     2161 atatcttcgg ctggaaagcc aacttcatca accaagacac gataagaacg ttcgcaaatt
     2221 tcgcgtttac gtgcagcggt atcagcttgg ccttgctcat caaaggccat gacaataatg
     2281 gctgcaccat aacggcggca caatttggct ttttgcacaa actcttcgtg gccttctttc
     2341 agcgaaatgg agttaacaat cggtttgcct tgtacacact ttaaaccagc ctcaatgatt
     2401 tcccacttgg aggagtcaat cataatcggc acacgcgaaa tgtcgggttc agaggcaatc
     2461 aagttcaaaa aagtgaccat tgccgcttgc gaatccaaca tgccttcgtc catgttgatg
     2521 tcgataattt gcgcgccact ttcaacttgt tgtcgtgcca catctaaggc ttcggcatag
     2581 ttttgttcac gaatcaaacg cgcaaacttt tttgagcctg tcacgttggt acgttcaccg
     2641 acgttaacaa ataacgaatc tttggtgatg ttataggctt ctaaacctga taaacgacaa
     2701 gcaggttcaa tctcaggaat aacgcgaggt gcaaggtctt ttacggcatt ataaatggcc
     2761 gaaatatggt cgggtgtagt accacaacaa cccccaacaa tgttgactaa accactttgg
     2821 gcaaactcac caaccattgc cgcagtttca gcaggggttt cgtcatattc accaaaggca
     2881 ttaggcaagc ctgcattagg gtgtgcagaa acgaaaatat cggctttttc gcttaattct
     2941 ttaatgtaag ggcgcatggc atctgcaccc aacgcacagt taaaaccaat acttaataag
     3001 tccccatgac gcaccgaatt ccaaaaagct tcggcagttt gacccgataa agtacggcca
     3061 gacgcatcgg taatcgtgcc agaaatcatt acaggtaaaa tagtgcctgt gtcatcaaaa
     3121 tatttttgta cggcaaacaa ggccgcttta cagtttaggg tgtcaaaaac ggtttcaatt
     3181 aacaaaatat ctgcgccacc atcgactaag cctttggttg cttccacata gttttcgact
     3241 aactcatcaa aggtgacatt acgataagca ggattattga catcaggcga aatagagcaa
     3301 gtccgtgacg ttgggcccag tacaccagca acaaaacgtg gtttttcggg ggtttttgca
     3361 ctccacgcta aggcgacttg cttggccaaa cgcgctgctt ccacatttaa ctcataaacc
     3421 aacgcttcca tatggtagtc agcttgcgag acttgggtgg agttaaacga gttggtttct
     3481 aaaatgtctg cacctgccgc acaataagca tcatgaattt cggcaatgac attgggcttg
     3541 gttaagacta ataaatcgtt attacctttg acatcatgtg gccaatcggc aaaacgtgac
     3601 ccacgataat ccgcttcttg taatttataa cgctgaatca tagtgcccat cgcaccatca
     3661 ataatcaaaa tgcgttgttt taaagcatct tttaataagt cagaacgaga agctgtcatg
     3721 gtgtatatct atcagtcatt aaaacgaggg cagctatttt aacaaaaatg tcttcatttc
     3781 gcacaaaaca attattaatc ctgagctaat tactaggtta ttaaaaatca ggtataatcg
     3841 ggcaaaattt tctattccga gaaggttatg agcaatctaa acattaacat tactgaatct
     3901 gcccaagctt atttgcgtga attattagca aaacaaaata gtgaaggcat tggcgtgcgt
     3961 atttttgttg aaaatgcagg cactcctaaa gccgaatgtt gtatggctta ttgcacccaa
     4021 ggtgaagagc aaattgatga tattcgtttg ccaatggagg gttttcctgc ctatatcgaa
     4081 tttaaaagct ctccttattt agaagatgcc gtaattgact acaacaaaga ccgttttggt
     4141 ggtcaattaa cctttaaagc acccaaatct aaagtgcctt tattgggtga aaatagttca
     4201 ctcgaagagc gtattaacca tgtgttgttt agcgaaatca atcctagctt ggccgcacac
     4261 aatggtaatg ttagcttatt agacgtgtat gaagacaccg agcatggttt aaccgccgta
     4321 ttaaaatttg gtggtggttg tcaggggtgt tcagccgttg atatgacctt aaaacaaggt
     4381 gtagaaacga ccttaaaagc gaatattcca gaattgcaac gggtgactga tagtaccgac
     4441 cacagtcaac gtgataacgc ctattttaaa taattccgta ggggcaaccc ttgtggttgc
     4501 cctttaattt ctcttatctt tctcttttaa cagttttttg cctgttaaaa tctccgaaac
     4561 accaaaacca aaaaatatta acgccattcc ccacatacca aataaatcaa acatggctgc
     4621 accacgagta ccattgagtt cgcctaaatc aatcccatgt ttttcggcat caaggccaat
     4681 ataaatcatg gcaggagcca ttaagactaa aaagatacct aaaatcattc taaaaatagc
     4741 catgtgattt tctcattata ttgattggta ggggcaaccc ttgtggttgc ctttcatgcc
     4801 tcgtagttag cttttatttt tgagtcttcc tatgctaaac agcttgcctt agaggtgcaa
     4861 ccatcgtcac aggtggtggt gtgacaggtt taggttttac tttaacagga ttaacaataa
     4921 aatttgtacc taataatatc aacgtcacgc ctaacacctt acgaatcgca cgctcaggca
     4981 agcgagagct taataatgtg cccacaataa tcgcaggaat agaacccatt aataacaaca
     5041 tcaataaatg ggtatcaacg ttgccagctt ttaaatgtcc cagaccagca acaagagtta
     5101 acaacactgc atgagccaca tcagaaccaa taatgcgaat catgggtaat ttaggaaaca
     5161 ataacactaa agccatgatg ccaaatgcac ctgcaccaac tgacgataaa gtgacaaaaa
     5221 agcctaaaaa tatgcccatt aacactaccc aacctttatg tttgagtaca acttggctat
     5281 gttcatcggc agttaatgct tcaccagcaa catcatgttg atgacgatta aaaaaagctt
     5341 caattttttg tctaaaaaca atagaaatac ctgttaaaac cagcattccg cccaaaataa
     5401 tcgttaacag ctttttgtat tctttcgcac cacccataaa atgttctaaa gcccatgtgg
     5461 ttgctaatga agctgggata ctgcctaaac ccaaccaaaa cacaatcggc caaacaatat
     5521 tcagtttttt ggcatgcact aaagagccac aaaatttaga aatagcggca tacagtaaat
     5581 ctgtaccaat ggcaatatgt ggttcaactc gtaaaaaact aattaaaatt ggagtcatta
     5641 atgagccacc gcccacacct gttaccccaa cacaaaagcc aacgactaag ccagctaaaa
     5701 taaaataaaa atcaggcatg atcaaaaaag tctatttttg taaagagcgg ctactgtaac
     5761 caatttctta tattgctaaa ataattttta gctatatgct tataactata tggcatataa
     5821 agaaaaggta aaaacgatga agttacagca gttacgttat atttgtgagg ttgccagtca
     5881 tgaactgaat atctccaccg ctgcacaaaa tttatttacc tctcaaccgg gagttagtaa
     5941 acaaattaga ttattggaag atgagttggg cgtagaaatt tttacccgta gtggcaaaca
     6001 tttaacgcaa attaccccag caggacaaga agttttaaag atagcacatg aaattctgcg
     6061 tcaggtagaa aatattcgtc aggtgggtga ggaatatagt aatccgtata tgggaagatt
     6121 agcgattgct accactcaca ctcaagcacg ttatcgttta ccacctgtga ttgaacaatt
     6181 tagaaaacga tatcctgaag tgagtttgca tttacagcaa ggcacaccgc aacaaattgc
     6241 tcaaatggcc tcttcaggat tagtagattt tgccattgct accgaagcaa tggagcactt
     6301 taataacctg attttattgc catgttatcg ttggaataga tcagtcgttg tgccaaaaaa
     6361 tcatccactc actaaattgc cacaactcag cttagaagca attgcccaat atccaattgt
     6421 gacttatgtc ttcgggttta caggtcgttc acaacttgat gatgctttta gccaacacgg
     6481 tttaacgcct aaagtggtat ttacggcaac agacgcagac gttatcaaaa cctatgttaa
     6541 gttagggatg ggggtgggaa tcattgccca catggcctat gagccacata ttgatacaga
     6601 tttggtcgcc ttagatgtga gtcatctttt tgcatcaagt accaccaaaa ttgcttttag
     6661 aaagggcatg tggttgcgga gttttatgtt tgattttatg gcagcttttg cgcctcattt
     6721 aaccagagca cgagtagaag ccgcaatttt ggcagaaacg acagaagagc gagagcaaat
     6781 ttttgccaag gttgagttgc cagttttgta gattttcctt cgacttcgtt taggacacat
     6841 acggtggctg agcgaagtcg aagccaaatt ctataaaaag tctaaatctt gactattatt
     6901 cattaattct ttgagttctt ctttttcatt agtcgtggca ttgagcactt ctttaacaaa
     6961 ctttaaatac acggtataaa tggtttggct accatcatct tggctcacca caaaaaacgg
     7021 tgcacggtca acgccaagtt tttttgctaa ttgcatacct gctgattcag gatcacgctc
     7081 atcagccact aaaatttcat tcataaattt taattggtca tcagcttcca tttttgccaa
     7141 cacttcacca cattttttgc aaggtgagcc atctgctaaa atctttttga ccattgtaat
     7201 gtgcattata aaatcctcat ggttcccttc cttttttaag gggagggcta gggtgtggtt
     7261 actccctctc ccacagggag aggggtgggg tgagggatac ccaaacccaa cttcccccta
     7321 aattaggggg aggagcaatt acttaatatt taccgcatgt aaaccacatt ctttctttgt
     7381 ggcttcttcc caccaccagc gaccttcacg ctcgtgttgg tttggtaaaa tagcgcgtgt
     7441 acaaggctca caaccaatac tggtaaagcc tttttcatgt aaagcattgt aaggcacttc
     7501 gttgctacga atataatccc acacttgtgc agaagaccag ttagacaaag gattaaactt
     7561 aattaattgt ttgccagcac cactaaacgc agcatcagct tgtactacag gcacagctaa
     7621 acgtgtacct gggctttggt ctttacgttg gcctgtaatc cacgcatcta aagtcgctaa
     7681 ttttttcttt aaaggcgcaa ctttacgcac agtacagcac tctttatgat cgtcagcata
     7741 aaagctaaac aaacctttag cattgaccat ttcttgcact tgtgcagatt caggaaaaca
     7801 aatttcgata ttgactttat agtgattgcg tactttttca ataaattgat aagtttcagc
     7861 gtgtaaacga cccgtatcta agctaaatac acgaaatggc ttgcccaatt tagcggccat
     7921 atcaatcaat acaacatctt ctgcaccact aaaagaaata gcaacatcag gaaagttctc
     7981 taaagcaagt gctaaaattt cttgaggggt tttttcttca taatcagttg cccattgttg
     8041 agcattggca tgagtaatgg tcatggctgt tcctctatat gttcggaaat ggctaaaagc
     8101 tgttgtacac agcaatcgtg aaaaactcta attctaacag catttagtga ttgcttgcag
     8161 cattctatta aaaacgctac agttcgcctg tttttt
//
LOCUS       DAOQMB010000061         8114 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3558_length_8114_cov_14.418911, whole genome shotgun sequence.
ACCESSION   DAOQMB010000061 DAOQMB010000000
VERSION     DAOQMB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3558_length_8114_cov_14.418911"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1330
                     /gene="tig"
                     /locus_tag="PLY05_03645"
     CDS             26..1330
                     /gene="tig"
                     /locus_tag="PLY05_03645"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNC02429.1"
                     /translation="MQVSVENVSGLERRVKIAVPADQVEQAVNKKINQAARTIKMDGF
                     RVGKVPLNVIKKRYGASIRAEALDDIIRDAYVSAIQQTELKVAGFPNIEPISFAEGKD
                     VEFAAVVEVYPEVTLGDFSALAVERPTSEVTDADVDTMIANLRRQRAKWEESAEAAAD
                     QDRLTIDFEGSVNGEKFEGGTAQDFAIVIGSKRMIPGFEEQLVGSKAGEEKVITVTFP
                     ADYQAENLAGKEAQFKINVKKVVKPTLPELDATFLEAFGVKDGNVDQFRADVRKNMER
                     ELRNGIRAKVKSAVFDALVKANSIELPKALVAEEINRQRQQAIQQFGGKNAQIKPEML
                     PDELFKEQSERSVALGLLVSEIIRANEITVDAERVRALVDEVAQSYEQPEEVVKWYYS
                     SKEQMAQVQSAVLEDQVVDLVLAKAQITDKAVGYEELLRPQA"
     gene            1649..2254
                     /gene="clpP"
                     /locus_tag="PLY05_03650"
     CDS             1649..2254
                     /gene="clpP"
                     /locus_tag="PLY05_03650"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HNC02430.1"
                     /translation="MMIQTINSGLVPMVVEQSSRGERAFDIYSRLLKERVIFMVGEVE
                     DHMANLIVAQMLFLESENPDKDIHLYINSPGGSVTAGMAIYDTMQFIKPNVSTICVGQ
                     ACSMGAFLLAAGAQGKRYCLPNSRVMIHQPLGGFRGQASDIAIHAQEILKIRSRLNDC
                     LALHTGQPLAQIEKDTDRDNFMGAEEARQYGLIDAVLEKRI"
     gene            2318..3622
                     /gene="clpX"
                     /locus_tag="PLY05_03655"
     CDS             2318..3622
                     /gene="clpX"
                     /locus_tag="PLY05_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNC02431.1"
                     /translation="MTDDRKSRNEKLLFCSFCGKSQHEVTKLIAGPSVYICNECVDLC
                     NDIMAQEPEEQSEAGKQKKEALPVPHEIKHTLDDYVIGQEKAKKVLAVAVYNHYKRLR
                     AGAKKAVNNKKSTEVELAKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEA
                     GYVGEDVENIIQKLLQKCDYDVEKAERGIVYIDEIDKITRKSENPSITRDVSGEGVQQ
                     ALLKLIEGTVASVPPQGGRKHPQQEFLQVDTSNILFICGGAFAGLEKVILNRSEKSGI
                     GFNATVRSKEEEKNTGEIFKEVEPEDLIKFGLIPELIGRLPVIASLEELSEDALIQIL
                     TEPKNALTKQYQKLFDMEGVGLEFSQDALVATAKKAMERKTGARGLRSIMENALLETM
                     YDLPSIEGISKVVVEERTITDDAKPLLVYNNDQASDAELKAS"
     gene            3888..>4255
                     /locus_tag="PLY05_03660"
     CDS             3888..>4255
                     /locus_tag="PLY05_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNC02432.1"
                     /translation="MYESELLDLPLLPLRDVVVYPHMVIPLFVGREKSIQALEAAMAG
                     HKKIFVVAQRDAADDDPQQKDLYDIGTVATILQLLKLPDGTVKVLVEGQRRAQVDEIN
                     DTGRYLLATVRELNPEPISAT"
     assembly_gap    4256..4355
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4356..>5995
                     /gene="lon"
                     /locus_tag="PLY05_03665"
     CDS             <4356..>5995
                     /gene="lon"
                     /locus_tag="PLY05_03665"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNC02433.1"
                     /translation="IHKELGELNDGGTGADNEFAELEQRIDKAGLPVEARKKVDSEFK
                     KLRLMPPMSAEATVVRGYIDWMLSVPWKKRSRIGIDLKMARQILDADHYGLEEVKERI
                     LEFLAVQSRVKKLQGPVLCLVGPPGVGKTSLGQSIAKATKREFVRMALGGVRDESEIR
                     GHRRTYIGSMPGKLIQRLAKVGVKNPLFLLDEIDKMGVDSRGDPASALLEVLDPEQNQ
                     HFNDHYLDVDYDLSEVMFLCTANSMDIPAPLLDRMEVIRLPGYTEDEKVNIAKTYLIP
                     KQIGLAGLSVKELKIEESAVRELVRRYTREAGVRNLEREISKVCRKVVKEAVLGQANS
                     EQVNVTADNLEQYCGVYKYSFGKAEEKDQIGQVTGLAWTSVGGELLTIEAVSTIGKGR
                     FTMTGKLGDVMQESIKAAITVVRSRSKMLGISPEFHEQQDLHVHIPEGATPKDGPSAG
                     IGMCTALVSVLTGIPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPEE
                     NARDLKDIPDNIKADLTIKTVKWIDEVLAIALTKQPTP"
     assembly_gap    5996..6095
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6096..7313
                     /locus_tag="PLY05_03670"
     CDS             <6096..7313
                     /locus_tag="PLY05_03670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC02434.1"
                     /translation="TEMHFLFIRILNMTTNKIEQVSRLAKTSAAFLKNAVQRRVNKQD
                     YILSDKTENEVIFQDDIMSVKYYKPLTAETLVLESGQSLHVRRRRFAIPIVMVPPLGV
                     FAWIFDLLVDRSLVRYFLAHGFEVYLIDWGKPTTDHADLSLENYVNDWFPKALAAVRE
                     HSDSDELSLLGYCMGGLLALMYLASHPEDDKVTNLITIASPIDMHKANPITGPISQLL
                     SMPASLIKNKTGFKLDNMSSDKFHVDGKTLSWLFKMTNPLGVVSSYIELIRNLADDDY
                     VARYMTMNEWFTNMPDYPGATVQDMMRKMGLYNRMAKGKLKIGERLADFSQIKTNLLA
                     FAGDNDKIVSIAGARAIMTIVSSTDKTFHIVPGGHAGVFTGSKAVHTTWSISKDWLQL
                     RSKAYPRPVQKAG"
     gene            7313..7852
                     /locus_tag="PLY05_03675"
     CDS             7313..7852
                     /locus_tag="PLY05_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNC02435.1"
                     /translation="MMSNIRPFDGVLPKVAESCFVDSTAVVIGDVELAENSSVWPCAV
                     IRGDVNSIKIGEGSNIQDFAMLHVTHKRPVDPLGAPLVIGKHVTIGHHVTLHGCTIGD
                     EVLIGIGTIVLDRVVIESQVLVGAGSLVPPNKRLESGYLYLGNPVKKVRALTDEEKAY
                     FKYSAEHYMRLANKHKAGV"
BASE COUNT         2378 a         1313 c         1804 g         2419 t
ORIGIN      
        1 agttgcttta tctttagagg atgtcatgca agtttctgtt gaaaatgttt ctggcttaga
       61 gcgtcgcgtt aaaattgctg tgccagccga ccaagttgaa caagcggtta ataaaaaaat
      121 taatcaagct gcgcgtacca ttaaaatgga tggtttccgt gttggcaaag tgccattaaa
      181 tgtcattaaa aagcgttacg gtgcaagcat tcgtgctgaa gctttagatg acattattcg
      241 tgatgcttat gtaagtgcca ttcaacaaac tgaattaaaa gttgcaggct tccctaatat
      301 tgaaccaatc agctttgctg aaggcaaaga tgttgagttt gctgctgttg ttgaagttta
      361 tccagaagtt actttaggtg atttttctgc attagccgtt gagcgtccta cttctgaagt
      421 aacagatgct gacgtagata cgatgattgc taacttgcgt cgtcaacgtg ctaagtggga
      481 agaaagtgcc gaagcagccg ctgatcaaga tcgtttgact atcgattttg aaggctctgt
      541 taatggtgaa aaatttgaag gcggtacagc acaagacttc gctattgtta ttggctctaa
      601 gcgtatgatt cctggctttg aagagcaatt agtcggtagc aaagcaggcg aagaaaaagt
      661 cattactgtg actttcccag ccgattatca agctgaaaac ttagcgggta aagaagcaca
      721 attcaaaatc aacgttaaaa aagtcgttaa accaactttg ccagaattag atgcaacttt
      781 cctagaagca tttggcgtta aagatggtaa cgttgatcaa ttccgtgctg atgttcgtaa
      841 aaacatggag cgtgagttac gcaatggcat tcgtgcaaaa gtgaaatctg ctgtatttga
      901 tgctttagtc aaagctaata gcattgagtt gccaaaagct ttagttgccg aagaaattaa
      961 tcgtcagcgt caacaagcta ttcaacagtt tggtggcaaa aatgcccaaa ttaaaccaga
     1021 aatgttgcca gatgaattgt ttaaagaaca atctgagcgt tctgttgcct taggcttgtt
     1081 ggtttctgaa attattcgtg ctaacgagat caccgttgat gctgagcgtg tacgtgcttt
     1141 agtggacgaa gttgcacaat cttatgaaca accagaagaa gtagttaagt ggtactacag
     1201 ctctaaagag caaatggctc aagtacaatc ggctgtttta gaagatcaag ttgtggattt
     1261 agtattagct aaagcacaaa ttactgataa agcagttggt tacgaagagt tattacgccc
     1321 acaagcttaa ttattcgata ttcaaaaaac cccagaaatg gggttttttt gtttgtgtta
     1381 tggtaagtgt gagtgtaatt tgctagctta attatatatt ttctgttaaa aaatattttt
     1441 acaaatttag agagtataaa gcttgggttt ttacagaaat tagcgttttg tgtgagctaa
     1501 tgttagtgaa attgccaaaa gatgcccaac atgaatatat ccaataaaaa gttattatta
     1561 aagattagcc tttaaatttg gaacaatcgt ccttatgttc ttagggcgag tgataatggg
     1621 ttctaactaa acaatttaca agagatatat aatgattcag acaattaata gtggccttgt
     1681 gccaatggtg gttgagcaat cttcccgtgg tgaacgtgct tttgatattt attcccgttt
     1741 attaaaagaa cgcgtcattt ttatggttgg tgaagtggaa gaccatatgg ccaatttaat
     1801 tgtggctcag atgttgtttt tggaatctga aaatcctgat aaagatattc atttatatat
     1861 caattctcca ggtggttcag tcactgcggg tatggctatt tatgacacaa tgcagtttat
     1921 taagcctaat gtgagtacca tttgtgtcgg tcaagcttgt tcgatgggtg cattcttatt
     1981 agcagcaggt gctcaaggaa aacgttattg tttgcctaat tctcgtgtga tgattcacca
     2041 acctttaggt ggcttcagag gtcaggcatc tgatattgca attcatgcac aagaaatttt
     2101 aaaaattcgc tcacgtttaa atgactgttt agcgttacat acgggtcagc ctttagcgca
     2161 aatcgaaaaa gatactgatc gtgataactt tatgggtgct gaggaagcac gtcaatatgg
     2221 cttgattgat gcggttttag aaaagcgtat ttaattggct caggacgagt taaagtgggg
     2281 cttggcttaa gcccctaata gtaattgagg aattgtgatg actgatgatc gtaaaagccg
     2341 caacgaaaaa ttattgtttt gttcattttg tggcaaaagc cagcatgaag taactaagct
     2401 gattgcaggt ccttcggtgt atatctgtaa tgaatgcgtc gatttgtgca atgatattat
     2461 ggcgcaagag ccagaagagc aaagcgaagc aggcaaacaa aaaaaagaag ccttaccagt
     2521 cccacatgaa attaaacata ctttagatga ttatgtgatt ggccaagaaa aagccaaaaa
     2581 agtcttagcg gttgctgttt ataaccatta taaacgtttg cgcgcagggg ctaaaaaagc
     2641 ggttaacaat aaaaaatcca ccgaagttga attggcaaaa agtaatatct tattaattgg
     2701 gccaaccggt tctggaaaaa cattattagc agaaacctta gcgcgtttgt tagatgtacc
     2761 gtttaccatg gctgatgcga ccaccttaac cgaagcaggt tatgtggggg aagacgttga
     2821 aaacatcatc caaaaattat tgcaaaaatg cgattatgat gtagaaaaag ctgagcgcgg
     2881 tattgtctat attgatgaaa ttgacaaaat cacacgtaaa tcagaaaacc cttctattac
     2941 ccgagatgtg tcaggtgagg gcgtacaaca agccttgtta aaactcattg aagggacagt
     3001 ggcttctgta ccgcctcaag gtggtcgtaa acacccacaa caagagtttt tgcaagtaga
     3061 tacgtcaaat atcttgttta tttgtggtgg cgcatttgca gggcttgaaa aagttatttt
     3121 aaatcgctca gaaaagagtg gtattggttt taatgccacg gtacgcagta aagaagaaga
     3181 aaaaaataca ggcgaaattt ttaaagaagt tgagcctgaa gatttaatta aatttggttt
     3241 aattccagaa ttaattggtc gtttgcctgt cattgcttct ttagaagaat taagcgaaga
     3301 tgctttaatt caaatcttaa ccgagcctaa aaacgcgctt accaaacaat atcaaaaatt
     3361 atttgatatg gaaggagtag gtttagagtt tagtcaagac gctttagtag caaccgctaa
     3421 aaaagcgatg gaacgtaaaa cgggtgctcg tggtttgcgc tctattatgg aaaacgcttt
     3481 gctagaaact atgtatgact tgccttctat cgaaggtatt agtaaggtgg ttgttgaaga
     3541 gcgtactatt accgatgatg ccaaaccgtt attagtttat aacaatgatc aagctagtga
     3601 tgcagagtta aaagcgtctt aatgatgatt ttgggtcttt attgtgtggg cagactttga
     3661 gtctgccttt attttttacg ctattttttt tcacttccct tgtaatttgc caaaaactcc
     3721 ctaaaacaat acaaatagaa taagacttac gcgttgcgcc gcaattagct tgttctgtga
     3781 agataaaatc gttacaagcg attgtaagca caaataagta ataccgcgta agtcctatag
     3841 aagataaata gagacagcca tgtttaagtg gttgtgggag ttaagatatg tacgagtcag
     3901 aattgcttga tttaccttta ttgccattac gtgatgtggt tgtgtatcca cacatggtta
     3961 ttcctttatt tgttggcaga gagaagtcaa ttcaggctct agaagcagca atggctggcc
     4021 ataaaaagat ttttgtggtt gcgcagcgag atgcggctga tgatgatcct caacaaaaag
     4081 atttatatga tattggtact gttgccacca ttttacaatt actcaaatta cctgatggca
     4141 cagtgaaagt gttggttgaa ggtcaacgtc gtgcgcaggt tgatgaaatt aatgatacag
     4201 gtcgctattt attggcgact gttcgtgagt tgaacccaga acctatttct gcgacnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnattca taaagagttg ggtgagctta
     4381 atgacggtgg gacaggggca gataacgagt ttgctgagtt agagcaacga attgataaag
     4441 caggtttacc tgttgaagca cgcaaaaaag tcgatagcga atttaaaaaa ctacgcctaa
     4501 tgccacccat gtctgctgaa gcaacagttg tccgtggtta cattgattgg atgttgagtg
     4561 taccttggaa aaagcgtagc cgtattggta ttgatttaaa aatggcacgt caaattttgg
     4621 atgccgatca ttatggctta gaagaagtca aagagcggat tttagagttc ttagctgttc
     4681 aatcacgggt gaaaaaatta caagggcctg tgctttgttt ggttggccca ccgggtgtgg
     4741 gtaaaacttc tttagggcaa tctattgcta aagcaactaa gcgtgagttt gtgcgtatgg
     4801 ccttaggtgg tgtgcgtgat gagtcagaaa ttcgtggcca tcgtcgtacc tacattggtt
     4861 ctatgcctgg taaattgatt caacgtttag ccaaagtggg tgttaaaaac ccattattct
     4921 tgctcgacga aattgacaaa atgggcgttg atagccgtgg tgatcccgca tcagccttgt
     4981 tagaggtatt agaccctgaa caaaaccaac actttaacga tcattattta gatgtggatt
     5041 atgatttatc tgaagtgatg tttttgtgta ccgctaactc aatggatatt ccagcacctt
     5101 tattagatcg tatggaagta attcgtttac ctggttatac cgaagacgaa aaagtaaaca
     5161 tcgccaaaac ctatttaatt cctaaacaaa ttggtttggc aggtttatca gttaaagagt
     5221 taaaaattga ggaaagcgcc gtccgtgaat tagtacgccg ctatacccgt gaagcgggtg
     5281 tacgtaactt agagcgtgaa atttctaagg tatgtcgtaa agtggtaaaa gaagcggtgt
     5341 taggccaagc taactcagag caagttaatg ttactgctga taatttagag caatactgtg
     5401 gtgtctataa atatagcttt ggtaaagccg aagagaaaga tcaaattggt caagtgactg
     5461 gtttggcatg gacatccgtt ggtggtgaat tattaaccat tgaggccgtt tcgacgattg
     5521 gtaaaggccg ttttaccatg actggtaaat tgggcgatgt gatgcaggag tcaattaaag
     5581 ccgcaatcac tgttgttcgt tcacgctcaa aaatgttggg gatttcacca gagtttcatg
     5641 agcaacaaga cttgcatgtg catattcctg aaggtgcaac acccaaagat ggcccaagcg
     5701 caggtattgg tatgtgtact gccttagtat cagtattaac gggcattcca gttcgtgcgg
     5761 atgtggcaat gacaggtgaa attaccttac gcggtcaagt gttaccgatt ggcggtttaa
     5821 aagagaagtt attagcagcg catcgtggtg gcattaaaac agtattaatc cctgaagaaa
     5881 atgcccgtga tttaaaagat attcctgaca atatcaaagc tgatttaacc atcaaaaccg
     5941 taaaatggat tgatgaggta ttagcaatag cattaaccaa acaacccaca cctttnnnnn
     6001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacaga gatgcacttt ttgtttatta
     6121 ggattttgaa tatgaccacc aacaaaattg agcaagtgtc ccgtttagca aaaaccagtg
     6181 ccgcattttt aaaaaatgcc gtacaacgtc gtgttaataa acaagattat attttgtcag
     6241 ataaaaccga gaatgaagtc atttttcaag atgacattat gtcagttaaa tattataagc
     6301 cgttgacggc cgaaaccttg gtattagaaa gtggccagtc ccttcatgtg cgccgtcgtc
     6361 gttttgctat tccgattgtc atggtgccgc cgttaggtgt gtttgcatgg atttttgatt
     6421 tgttggttga ccgaagcttg gtgcgctatt ttctagcaca tggatttgag gtttatttga
     6481 ttgattgggg caaaccaacc acagatcatg ccgatttatc cttagaaaac tatgtcaatg
     6541 attggtttcc aaaagcttta gcggctgtgc gcgaacatag tgacagtgat gaattatcat
     6601 tactgggtta ctgtatgggg ggcttgttgg cgttaatgta tttagcctct catcccgaag
     6661 atgacaaagt gaccaattta attaccattg ctagcccgat tgatatgcat aaagccaatc
     6721 caatcactgg cccaattagt caacttttat caatgccagc aagtcttatt aaaaataaaa
     6781 cgggttttaa attagacaat atgtcttcag ataaatttca tgtggatggt aaaacgctgt
     6841 cttggttatt taagatgacc aatccattag gcgtagtgtc tagttatatt gagctaattc
     6901 gtaatttggc tgatgatgat tatgtggctc gttatatgac tatgaatgag tggtttacca
     6961 atatgcccga ttatcctggt gctaccgtac aagatatgat gcgtaaaatg ggtttatata
     7021 atcgcatggc taaaggtaaa ttaaaaattg gtgaacgttt ggcagacttt agtcaaatta
     7081 aaaccaattt attagccttt gcaggtgaca atgacaaaat cgttagcatt gcaggtgcgc
     7141 gagcaattat gaccattgtg tcatctaccg ataaaacctt tcatattgtt cccggtggtc
     7201 atgcaggggt gtttacaggc agtaaagcag tacataccac atggtcgatt tctaaagact
     7261 ggttgcaatt gcgttctaaa gcctatccac gccctgtgca aaaggcaggt taatcatgag
     7321 caatattcgt ccttttgatg gcgtgttgcc aaaagtagca gaaagttgtt ttgtggatag
     7381 tactgcggtg gtgattggtg atgttgagtt agccgaaaat agctcggtgt ggccatgtgc
     7441 ggtgattcgt ggtgatgtaa atagcatcaa aattggtgaa ggttcaaata ttcaagattt
     7501 tgctatgttg catgttactc acaaacgtcc tgttgatcct ttgggtgcgc cattagtaat
     7561 tggtaagcac gtcactattg gccatcatgt gactttgcat ggttgcacca ttggtgatga
     7621 agtgttgatt gggattggca ctattgtttt agaccgtgtt gtgattgaat ctcaagtatt
     7681 ggttggcgca ggcagtttag tgccacccaa taaacgctta gagtctggct atttgtattt
     7741 gggtaatcct gttaaaaagg tcagagcttt aaccgatgaa gaaaaagcct attttaaata
     7801 ttcggctgaa cattatatga gattggcaaa taaacataag gcaggggtgt aaagattctt
     7861 gcatcaaagt aatattgctt agattgtttt tggttttgct ttaacttcac gctacatttc
     7921 ggctgcggct aattgcttcc tcagtttgtg cataaagcag attttgcctt acagcaaata
     7981 gggcggtttg tgaaaagatg tgcataaaaa aagagccgtt aggctctttt ttatacttat
     8041 catattttta agcagtaacg ggttgtacca tttgttgcat caataactgc atacgcttgc
     8101 tttgcatttt taag
//
LOCUS       DAOQMB010000062         8085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3580_length_8085_cov_16.806351, whole genome shotgun sequence.
ACCESSION   DAOQMB010000062 DAOQMB010000000
VERSION     DAOQMB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3580_length_8085_cov_16.806351"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="PLY05_03680"
     CDS             <1..1224
                     /locus_tag="PLY05_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02436.1"
                     /translation="SLLEVAGFGPDLGKGPIRLSCRSQCAISWHAPRLWNPVMDSADN
                     TFVQLQKALPDACSRLGIRPSKVAAVAAAARKNPSTSLSFTATGIPVLTDAALQAASY
                     NQSQKLQELEQKIQDLTLRLQATVAEKEEQINLQSYVHQQQLDILQTQNMKLVEQNKN
                     LKNQQSTQQERLDALNSQVSSLSGIEDSLRQERAIHQKQLQELQLALSAAGEQKNEIA
                     TLLTNKDEEYQTRISRLKQEHLLSLDKRLEEEASRYLLSLKSLNAEIDELKETITQLE
                     QQLEQEKQTHNQEQEASADDFLVKLESLGMNFVVYHAGVGNLSVPVVDLVIYLQNPTA
                     YVAQKCLVTETHYRQWLQHYESPRCTAPIGEGQCCNARLIRTDSPSRFVVGQSDRCAR
                     HQGADTAIINVLKFN"
     gene            1242..2315
                     /locus_tag="PLY05_03685"
     CDS             1242..2315
                     /locus_tag="PLY05_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC02437.1"
                     /translation="MMTTALTTHILHNFTPLNVLSENRLKDVLTGCYVESVKPDTVLF
                     SYGDCDDKTIFLLSGELELIDYQGKVTLLNALDYAANYAISPSKPRQFTVKVKKPSNI
                     LAVDQQKLNDALLWEQSLSSLAKTLFAQLPENIDRDWLLLLLQSRIFYRLPPMNILEI
                     LASLEEVSVLEGQKIIAIGEEADSCYFIKKGKVQVCQNIDDEYLTVAYLGRGDFFGEE
                     GLLKDSPRNADVIMLEDCVLMRLVKKDFDRLLKKPTLQAFSFELANNQVQNQEAAWLD
                     VRLAEEFSLQHLTHALHLPLPDIRLKSRQLPLNQHYIICCDNGQRSAAAAFLLGIQGY
                     NVSVLSMGLEGLSAQQRQQYMVH"
     gene            2394..3284
                     /locus_tag="PLY05_03690"
     CDS             2394..3284
                     /locus_tag="PLY05_03690"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HNC02438.1"
                     /translation="MIKDSFRNIGLIGRPNSSLVIDTLNQLEAFLLHEGLTVIYDEDT
                     AQLMQKTGLQISSRALLGEVCDLVIVVGGDGTLLHAARSLARYKVPVLGVNRGRLGFL
                     TDINPDEVISKVNSVLHGHYTTDQRFLLNLEVRSNGSRVAEALALNDVVLHAGKSVHM
                     LEFELYIEGQFVYRQRSDGLIVATPTGSTAYALSGGGPIMHPKLDAIVLVPMHPHTLS
                     SRPIVVDADSEIKIRITETTLKPLVSPDGQSGISLNTGDWVYITKHPFKLKLIHPSGY
                     DFYAACRTKLGWGNPITGDD"
     gene            3284..3574
                     /locus_tag="PLY05_03695"
     CDS             3284..3574
                     /locus_tag="PLY05_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1315 family protein"
                     /protein_id="HNC02439.1"
                     /translation="MNLNHDTFAAILAALNPDIVASFKRAIELGKWPDGRRLSDEQKE
                     TCMQAVLVWEMKYLPEEERTGYINKGKKEGEVCDDHEHSHDQEIEQIIKFKH"
     gene            3626..5452
                     /locus_tag="PLY05_03700"
     CDS             3626..5452
                     /locus_tag="PLY05_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC02440.1"
                     /translation="MFSWFERRVDPYPANKPIPPQSGLIPFIYSCTEGVRVWIISLML
                     ATAAIGAFEAYLFSMMGQIVDWLSSLKPDQLWLEKKSDIILMSVLIIASPLLVLLQSL
                     LKFQALQGNFPMRLRWNFHRLMLDHSLGFYQEEFAGRVSAKVMQTALAVRDSIMTIAN
                     MVMFMLIYFVTCGIVLINFDWWLLAPFAAWIMLFGFALAYFIPRLGKTAERQADARAL
                     MTGRITDAYANISTVKLFSYSQRESSYAKRAMQEFMVTVHQQMRLVTQFETVNHSINM
                     TLVLGTTALALWLWFEQQVTVGAVAASTAMALRLNGISHWIMWELAMLFEHIGTVRDG
                     MGTLSKPHQIVDIEHARPLAITQGTIDFDNINFAYNSNKNVLENFNLHINAGEKIGVV
                     GRSGAGKSTLVNLLLRFYELDKGQISIDGQNIALVSQDSLRAHIGMVTQDTSLLHRSV
                     RDNLTYGRPDATDEQILAAIDKAQATDFIKSLKDAQGRTGLEAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQTSLYQLMEGKTVIAIAHRLSTIAAM
                     DRLIVVDDGKIIEQGTHAELLVKNGLYARLWARQSGGFLGEM"
     gene            5520..6737
                     /locus_tag="PLY05_03705"
     CDS             5520..6737
                     /locus_tag="PLY05_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNC02441.1"
                     /translation="MKIADFPSNEQERLGALLRYKVLDTDFEDTYNELTQLASDICQT
                     PIALISLIDRDRQWFKAKVGLEASETPRDWAFCAHALLQEDILVVEDALLDARFSDNP
                     LVINDPLIRFYAGAQLKTAQGLVLGTICAIDRVPRHLSEQQLHALKVLAKQVISQFEL
                     RLAFEELQAQAQRLHQLNATKDKLFSIISHDLRAPLSGIFSVSEILLEDIHHLELEDT
                     KQFINDIHEASQQSLSLLDNLLRWSLLETGKFNYQPVTIDLLAVIQKVLRLLNGAIVS
                     KRISLKIDCQANLSVVGDKNMLASVIQNLLSNAIKFSHPNGIIEINVIDNQDQTVLVS
                     IKDNGVGMTDEQQKQLFAVEKNQSTHGTQGETGTGLGLVLSYQFLKIHGSILTVKSEQ
                     GKGAIFSFKLNKS"
     gene            complement(6910..8001)
                     /locus_tag="PLY05_03710"
                     /pseudo
     CDS             complement(6910..8001)
                     /locus_tag="PLY05_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016539467.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
BASE COUNT         2469 a         1396 c         1705 g         2515 t
ORIGIN      
        1 agtttattag aggttgctgg ttttggtcct gatttaggta aaggtccaat acgtttatct
       61 tgccgtagtc aatgcgctat ttcttggcat gccccacgct tatggaatcc cgtgatggat
      121 tctgcggata atacctttgt gcaattgcag aaagctttac ccgatgcatg ttctcgctta
      181 gggattcgtc catctaaagt ggcggcagta gcagcagcag cgcgtaaaaa tccaagtacc
      241 tctttaagtt ttacggcaac aggcattcca gtattaacag atgcggcatt gcaagcagct
      301 tcttataatc aaagtcaaaa attacaagaa ttagagcaaa aaattcaaga tttgacgcta
      361 agattacaag cgactgttgc cgaaaaagaa gaacaaatca atttacaatc ttatgtacat
      421 cagcagcagt tagatatttt acaaacccaa aatatgaaac ttgttgaaca aaataaaaat
      481 ttaaaaaatc agcaaagtac ccaacaagaa cgtttagatg ctctaaatag tcaggtaagt
      541 agtctctctg gaattgaaga cagcttacgc caagaacgtg ctattcacca aaagcaatta
      601 caagagcttc agttggcttt gagtgcggct ggtgagcaaa aaaatgaaat tgcaacatta
      661 ttaactaata aagatgagga ataccaaacg cgtattagtc ggttaaaaca agagcattta
      721 ttatctttag ataaacgact tgaagaagag gcatctcgat atttgctgag tttaaaatca
      781 cttaatgcag aaattgatga attgaaggaa acgattaccc agttagagca acaattagaa
      841 caggaaaaac agacccacaa tcaagagcaa gaagcgagtg ctgacgactt tttagttaaa
      901 ttagaaagcc ttggcatgaa ttttgttgta tatcatgcgg gggttggtaa tttatctgta
      961 ccagtggtcg atttggtcat ttacttacaa aatccgacag cttatgttgc ccaaaaatgt
     1021 ctagtaaccg aaacgcatta tcgtcaatgg ttgcaacatt atgaaagccc ccgttgtact
     1081 gcacctatag gtgaggggca atgttgtaat gcacgattaa ttcgtactga ctcaccaagt
     1141 cgttttgtgg ttggtcaatc agaccgttgt gcacgtcatc agggggcaga tacagcaatc
     1201 atcaatgtac tgaaatttaa ctaggtgatg agatgggcat aatgatgaca acagcgctca
     1261 caacacacat tttacataat tttacccctt taaatgtctt atctgaaaat cgtttaaaag
     1321 atgttttaac gggttgttat gttgaatctg ttaagccaga tacagtgctt tttagctatg
     1381 gtgattgtga tgataaaact atatttttat taagcggtga gctggagtta atagattatc
     1441 aaggaaaagt aacgttatta aatgccttag attatgccgc taattatgcg atttctcctt
     1501 caaaaccacg tcaatttacc gttaaagtaa aaaagccatc aaatatttta gccgttgatc
     1561 aacaaaaact taatgatgct ttgttgtggg aacagagttt aagtagctta gctaagactt
     1621 tatttgcaca attgccagaa aatatcgata gagattggtt gttgttgtta ttgcaatcac
     1681 gtattttcta ccgtttaccg cccatgaaca tcctagaaat attagcttct ttagaggaag
     1741 tttctgtgct agaaggacaa aaaatcattg ccattggtga agaggcggat agttgttatt
     1801 ttattaaaaa agggaaagtt caagtttgtc aaaatattga tgatgaatat ttgacggtgg
     1861 cttatttggg acggggagat ttttttggtg aagaagggtt gctcaaggat tcaccacgta
     1921 acgcagatgt gattatgctt gaagattgcg tgttaatgcg tttagttaaa aaagattttg
     1981 atagactgtt aaaaaaacca actttacaag cctttagttt tgaattggca aacaatcaag
     2041 tacaaaatca agaggctgct tggctcgatg ttcgcttagc agaagagttt agcttgcaac
     2101 atttgacgca tgctttacat ttacccttgc cagatattcg tttaaaatct cgtcaattac
     2161 cgctgaatca acattatatt atttgttgtg ataatggtca gcgcagtgct gcagccgctt
     2221 ttttattagg catacaagga tataatgtct cggttttaag catgggctta gagggtttat
     2281 ctgcgcagca acgacaacag tatatggtgc actaaacatt tttagtcaac aatatcctat
     2341 cttttgtttg tataatgtag cgtataaaat attgtgcact gctgagcata tacatgataa
     2401 aagatagttt tcgtaatatt ggcctaatcg gtcgtcccaa tagttcacta gtcatagaca
     2461 ccttaaatca gttagaagct tttttattgc atgaaggatt aaccgttatt tatgatgaag
     2521 atacggcaca acttatgcaa aaaacaggct tacaaatttc ttcacgagcg ttgttaggtg
     2581 aagtctgtga tttggtgatt gtggttggtg gtgatggcac attattacat gcagcacggt
     2641 cattggctcg ttacaaagtg cctgttttag gtgttaaccg tggccgttta ggctttttaa
     2701 ccgatattaa ccctgatgaa gttattagca aagtaaactc agtattgcat ggtcattaca
     2761 ccactgatca acgttttttg ttgaatttgg aagtccgttc taatggtagt cgtgttgctg
     2821 aagctcttgc cctgaatgat gttgtgcttc atgcaggtaa gtctgtgcat atgttagaat
     2881 ttgagcttta tatcgaaggc caatttgttt atcgtcagcg ttctgatggc ttaattgttg
     2941 ccacgcctac aggttcaact gcttatgcgc tgtctggcgg tggtcccatt atgcatccca
     3001 aattagatgc tattgttttg gtgcctatgc atccacatac tttatcaagt cgcccgattg
     3061 tcgtagatgc agatagtgaa ataaaaattc gcattactga aacaacatta aaaccattag
     3121 taagccctga tgggcagtct ggtattagct taaatacggg agattgggtt tacattacta
     3181 aacatccctt taaattaaag ttaatacatc cctctggtta tgatttttat gcggcttgcc
     3241 gtaccaaatt aggttggggt aatcctatta caggagatga ttaatgaatt tgaatcatga
     3301 tacatttgca gcaatattag cagcattaaa tccagacatt gttgcaagtt ttaaacgtgc
     3361 tattgaacta ggtaaatggc ctgatggtcg tcgtttaagt gatgagcaaa aagaaacctg
     3421 tatgcaagct gtgttagttt gggaaatgaa atatctccca gaagaagaac gtacgggata
     3481 tattaataaa ggaaaaaaag aaggcgaggt atgtgacgat catgagcata gccatgatca
     3541 agaaattgag cagattatca aatttaaaca ctaatttttg tagacccaca cagtgggtct
     3601 tgttttttta tcgcaatggt tttttatgtt ttcatggttt gagcgtagag ttgaccctta
     3661 tccagccaat aaacctatcc cccctcaatc aggtttaatt ccatttatct attcatgtac
     3721 tgaaggtgta cgcgtttgga ttattagttt aatgctagcc actgccgcga ttggcgcatt
     3781 tgaagcctat ttatttagca tgatggggca gattgtcgat tggttaagct cattaaaacc
     3841 cgatcaactt tggttagaga agaagagtga tattatttta atgtcggtgt tgattattgc
     3901 tagtccgttg ctggtcttgc tgcaatcgtt attaaagttt caagccttac agggcaattt
     3961 tccgatgcgt ttgcgttgga attttcatcg tttaatgtta gatcatagct tagggtttta
     4021 tcaggaagag tttgctggcc gtgtttcggc aaaagtgatg caaacagcct tagccgttcg
     4081 tgactcaatt atgactatcg ccaatatggt catgtttatg ctgatttatt ttgtcacttg
     4141 tggcattgtt ttaatcaatt ttgattggtg gttattagca ccatttgctg cttggattat
     4201 gttatttggc tttgcattag cctattttat cccacgtttg ggtaaaactg ccgaacgtca
     4261 ggctgatgca cgcgcgttaa tgactggtcg aattaccgat gcttatgcca acattagtac
     4321 agtaaagtta ttttcgtata gtcaacgcga atcaagttat gctaagcgtg caatgcaaga
     4381 gtttatggtc accgttcacc aacaaatgcg cttagtgacc caatttgaaa ccgtaaacca
     4441 tagtattaat atgaccttgg tgttgggtac aacggcctta gcattgtggt tatggtttga
     4501 gcagcaagtg acggtggggg cagtggcagc ttcaacggcg atggctttgc gtttaaatgg
     4561 tatttcgcat tggattatgt gggaattagc catgttattt gagcatatcg gcacagtgcg
     4621 tgatggtatg ggaactttat caaaacccca ccaaattgtt gatattgaac atgccagacc
     4681 attagcaatt actcaaggga caattgattt tgacaatatt aattttgcct acaattcaaa
     4741 taaaaatgtg ctagaaaatt ttaatttgca cattaatgct ggcgaaaaaa ttggcgtggt
     4801 tggccgttct ggtgcaggca aaagcacctt agtcaattta ttattgcgct tttatgagct
     4861 agataaaggc caaattagta ttgatggaca aaacattgcc ttagtcagtc aagattcatt
     4921 acgtgcacac ataggcatgg tcacccaaga cacttcattg ttgcaccgtt cagtgcgtga
     4981 taacttaact tatggtcgtc cagatgccac tgacgaacaa attttagcgg cgattgataa
     5041 agcccaagca acagatttta ttaaatcttt aaaagatgcc caaggacgta ctggtttaga
     5101 agcgcatgtt ggtgaacgtg gtgttaaatt gtctggtggt cagcgtcagc gtattgcgat
     5161 tgcccgtgtg atgcttaaag atgcgccgat tctattatta gatgaagcca ctagtgcttt
     5221 agactccgaa gtagaagcag ctattcaaac cagtttatat caattaatgg aaggcaaaac
     5281 agtgattgcg attgcccatc gtttatcaac gattgcagcc atggataggt taattgttgt
     5341 tgatgatggc aaaattattg agcaaggcac tcatgcagaa ttattggtaa aaaatggctt
     5401 gtatgcgcgt ttgtgggcaa gacaaagcgg tggttttttg ggagagatgt agaggatttt
     5461 ttttagagct tatgatacct ttaaaaaata taaattaggg tctgttgaca ggagtcaata
     5521 tgaaaattgc cgattttcca agtaatgaac aagaacgact tggtgcatta ttacgctata
     5581 aagtgttaga tacagatttt gaagatactt ataatgaatt gactcaatta gcatcagata
     5641 tttgtcaaac acctattgct ttaattagtt taatagatcg tgatcgtcag tggtttaaag
     5701 caaaagttgg tttggaggcg agtgaaactc ctagagattg ggctttttgt gctcatgctt
     5761 tattacaaga agatatttta gtggtagaag acgcgttgtt agacgcaaga ttttctgata
     5821 atccactggt tattaatgat cctttaatcc gtttttatgc gggggcgcaa cttaaaaccg
     5881 cacaaggctt agtgttgggg acaatttgtg ccatagatcg tgtgccgcgt cacttatctg
     5941 aacagcaact ccatgcttta aaagtgttag ctaaacaggt gattagccaa tttgagctgc
     6001 gtttagcatt tgaagaatta caagcccaag cccaaagact acatcagctt aatgcaacca
     6061 aagataaatt attttctatt atttctcatg atttacgcgc acctttatca ggaatattta
     6121 gtgttagtga aatcttgtta gaagatattc atcatttaga gttagaagac accaaacaat
     6181 ttattaatga tattcatgag gcaagccaac aaagtttgtc tctgttagat aatttattgc
     6241 gttggtcttt gttggaaaca ggtaaattta attatcaacc agtcacaatt gatttgttag
     6301 ctgttattca aaaagtgttg cgtttattga atggtgctat tgtaagcaag cgcatcagtt
     6361 taaaaattga ttgccaagca aatctatcgg ttgttggtga taaaaatatg ttggcgtcgg
     6421 tgatacaaaa tttattatct aatgccatta agtttagcca tcccaatggc atcattgaaa
     6481 ttaatgttat tgataatcaa gatcaaacgg tattagtcag tattaaagat aatggtgtag
     6541 gcatgaccga tgagcaacaa aaacaattat ttgcggtaga aaaaaatcaa tcaacgcatg
     6601 gaacgcaggg tgaaacaggg actggcttgg ggttagtgtt aagttatcag tttttaaaga
     6661 tacatggctc aatcctaacg gttaaatcag agcagggcaa aggtgcgatc tttagcttta
     6721 agttaaataa gtcttaattg agtaaaattc ctagcgaaag ttttatggag taaatgtcgc
     6781 gtaaaacatt aaaaaagggc tgaaaatcag cccttatgat tacatgataa taatgccacg
     6841 tttttcccat tctttaagct gttcttcact aaagttatgc gctaataaca cgtttttagt
     6901 atcagagcct aacgctgttc cgcttaaatg atattgatta ggcgtcgcac taaatacaat
     6961 cggtgtggca atttggcgtt gagttttacc atttggtgtt ggaacgtcaa cgagcatttg
     7021 acgacttaat aattgagggt gttcacaggc ttctgcaaag gataaaactg gctcaacgca
     7081 agcttcaata gacgcaaaaa tagtaatcca ctcattaaag gttttttgga taatcgcttc
     7141 gcgaatagcg gctttgacaa aactaaccgc ttcttcatca tgacgaatag aaagcggaat
     7201 caaatcagca cggccaatcg catttgcaaa agccataaaa aattgtggtt ctaaaccacc
     7261 aacactcaaa taacggccat ctgctgtttg ataacaatca taaaaagtgc cgccatttag
     7321 cgtatccgtt tcgggtttag gctggtgatt agccattaat gcaggagggg cagttaaagc
     7381 ttgcaagcta aatgcggcat cagttaatga aatatcgaca aattggcctt cgcctgtgtt
     7441 ttgacgatga ataacggcgg ccaaaatacc agtcaccgca tataaaccac ctgtaatatc
     7501 tgcggcctga ataggtaaag gaactgggcc agatgctttg cgacctgtat agtccaaaat
     7561 ccctgcgatt gctaaatagt ttaggtcatg tcccgcacga tttttataag caccttgttg
     7621 tccataacca gtcaccgcac aataaattag ccgtggatta atagccttta gttgctcgta
     7681 accaatacct aaacgcgaca tcactgaagg acgaaattgc tcaacaacaa tatcgtattc
     7741 ctcgaccaac tttttgatta tttcaatacc ttctggtgtt tttagatcaa caacaatact
     7801 agatttatta cgatttaaag aaccatgagc ggcagaaagt ccttgctcat caaaaggagg
     7861 catcacacga atcatgtctg gccgagttgg agcttccact cgtaaaacgc ttgcgcccat
     7921 atcagctaaa atacgagtgc caaagggggc gggtaataat cctgaaaaat ctagaacttt
     7981 taaaccttta agtgcagaca tttttattcc tcttgatctc aattttggga caaactaatc
     8041 aacttacaat acgattgtac aaaatgttgt tgaggtctgc tagtt
//
LOCUS       DAOQMB010000063         8063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3596_length_8063_cov_17.137488, whole genome shotgun sequence.
ACCESSION   DAOQMB010000063 DAOQMB010000000
VERSION     DAOQMB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8063
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3596_length_8063_cov_17.137488"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="PLY05_03715"
     CDS             <1..540
                     /locus_tag="PLY05_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNC02442.1"
                     /translation="LEAQIHGGGHERGFRSGTLATHQIVGMGEAFRIAKEEGIAEQQR
                     LAVLRDKLWAGLCSLEQVFLNGDDKQRIAGNVNVSFNFVEGESLIMALKDIAVSSGSA
                     CTSASLEPSYVLRALGLSDELAHSSIRFTIGRFTTEEDIDFVINHAQNAVNKLRDLSP
                     LWDMYKEGIDLSQVQWAAH"
     gene            562..948
                     /gene="iscU"
                     /locus_tag="PLY05_03720"
     CDS             562..948
                     /gene="iscU"
                     /locus_tag="PLY05_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003651560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HNC02443.1"
                     /translation="MAYSEKVIDHYENPRNVGNLDKNDTHVGTGMVGAPACGDVMRLQ
                     IRVNDDGVIEDARFKTYGCGSAIASSSLVTEWLKGKTLDQAQQIKNAEIAEELALPPV
                     KIHCSVLAEDAIKAAIEDYRKKQTGE"
     gene            959..1282
                     /gene="iscA"
                     /locus_tag="PLY05_03725"
     CDS             959..1282
                     /gene="iscA"
                     /locus_tag="PLY05_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HNC02444.1"
                     /translation="MAVSLTPAAANHVSDFITNRGKGEGIRLGVKVSGCSGLAYVLEF
                     VDNADSADQVFESHGVKIFVDPKSLVYLDGIEMDFVKEGLNEGFKFSNPNQKGECGCG
                     ESFTV"
     gene            1402..>1925
                     /gene="hscB"
                     /locus_tag="PLY05_03730"
     CDS             1402..>1925
                     /gene="hscB"
                     /locus_tag="PLY05_03730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HNC02445.1"
                     /translation="MDKQDNYFAWFDLPVAFQINQDALASRYRELQRQYHPDRFANHP
                     DEQQKAVQWSAQLNTAYDILNSDVKRASYLLELSGRPISLNTSIADTDFLMSQLELRE
                     QLDEAEVVEQLVGLRLEVEEWLQSLSREFVLDYADEDWVEAQDTVRKMHFMANFLLDI
                     RQQEDKFEDDDYYD"
     assembly_gap    1926..2025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2026..3567
                     /gene="hscA"
                     /locus_tag="PLY05_03735"
     CDS             <2026..3567
                     /gene="hscA"
                     /locus_tag="PLY05_03735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HNC02446.1"
                     /translation="PYQFRAEQTGMPAFITAQGEKTPVEVSADILSVLKQRAEAALDG
                     ELKGAVITVPAYFDEAQRQATRDAATLAGLKVLRLLSEPTAAAVAYGLDHGAEGVHAI
                     FDLGGGTFDISLLRLHQGVFEVLATGGDAALGGDDFDRAIALWIMQQVDLAQPNAAQQ
                     RELMAIACQAKEQLTTQSTTTITWQTWQAELSVVQFNELITPLVEKTLRACKRALRDA
                     NLKANQILEVVMVGGSTRVPLVRQKVAEFFGRQPLTNIDPDKVVAIGAAIQADVLVGN
                     KPDSDMLLLDVIPLSLGLETMGGLVEKVVPRNTTIPVTRVQEFTTFKDGQTAMSIHVV
                     QGERELVQDCRSLARFELRGIPPMAAGAARIRVVFQVDADGLLNVSAIEVSSGQKSEI
                     TVKPSYGLHEQDISRMLTESYQAAEADKQARALHEEKVESQQLLEAIKAALREDGKLL
                     CEGELSTLHEGIKHLEIALGSTQRQIIEKARLALIPLSDAFAARRMNQHIHDALSGHK
                     LSDWE"
     gene            3571..3909
                     /gene="fdx"
                     /locus_tag="PLY05_03740"
     CDS             3571..3909
                     /gene="fdx"
                     /locus_tag="PLY05_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019384153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HNC02447.1"
                     /translation="MPNIIFLPHQQLCPDGAVVEAEVGETICDAALRAGLEIEHACEK
                     SCACTTCHVVVREGFDSLNEMDDLEADMLDKAWGLEPDSRLSCQAVVADEDLVVEIPK
                     YTINHASEKH"
     gene            3960..4160
                     /gene="iscX"
                     /locus_tag="PLY05_03745"
     CDS             3960..4160
                     /gene="iscX"
                     /locus_tag="PLY05_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HNC02448.1"
                     /translation="MSLKWTDSREIAIALFDAHPDVDPKTIRFTDLYNWVIALPEFAD
                     DPKHSGEKILEAIQMCWLEEAD"
     gene            4429..5541
                     /gene="tgt"
                     /locus_tag="PLY05_03750"
     CDS             4429..5541
                     /gene="tgt"
                     /locus_tag="PLY05_03750"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNC02449.1"
                     /translation="MQFKLLATDGIARRGELTLQHGKVQTPAFMPVGTYGTVKGMFPS
                     DIENIGADIILGNTFHLWLRPTTEVVKQFGGLHKFINWDKPILTDSGGFQVFSLGALR
                     KISEEGVKFQSPIDGSKVFLTPEVSMQIQRELNSDIVMIFDECTPYPATEKEARDSME
                     LSLRWAARCKTAHEGNPNALFGIVQGGMYEHLRDVSLAGLLDIGFDGYAIGGLSVGEP
                     KDEMLKILNHVPTKLPNDKPRYLMGVGKPEDIVEAVRRGVDMFDCVMPTRNARNGHYF
                     VTNGVVRIRNSQYKTDQSPIDEHCDCYACKNFSRAYLHHLDKCGEMLGAMLGTLHNLR
                     YYQRLMAGLRQAIEQGQLQAFVADFYARRELPVPSL"
     gene            5663..6025
                     /gene="yajC"
                     /locus_tag="PLY05_03755"
     CDS             5663..6025
                     /gene="yajC"
                     /locus_tag="PLY05_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNC02450.1"
                     /translation="MSFFIAEAYAAAPAAQEPSAIANIVMLVVFMLVFYFLLWRPQSK
                     RQKEHQALMTGLQKGDEVVFAGGLLGKIKSVDEDYAVVEINDNLTVKIQKASVLATLP
                     TGTLKGIGTGSSTNLSKD"
     gene            6088..7950
                     /gene="secD"
                     /locus_tag="PLY05_03760"
     CDS             6088..7950
                     /gene="secD"
                     /locus_tag="PLY05_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001270215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HNC02451.1"
                     /translation="MRYPLWKYILIVIVLLVSAIYSLPNLYPDEPAIQISGANAGVKA
                     DKTVLSQAETALKQAGLTFHGSEVQAKGVLLRVNTSDEQLKAQEIVRRALGDGYVVAL
                     NLAPTTPEWLLSLNAAPMKLGLDLRGGVHFLLEVDMSKVLEQRQETYATDIKAKFREA
                     KVAYRTVADITNGLTVKFAKEEDRTAAKELLAREYREFILVPAMRDNELYLDLAFAPE
                     KLKEFSDYAIEQNLTTLRNRVNELGVAEAVVQRQGSNRIVVQLPGVQDTAEAKRVLGR
                     TATLEFRFVDWEHQGAVDGIAPAGTEIFPMNKTKEPVLLQRKKIVTGDRVVNAQSGFD
                     EYSNPQVNITLDGRGGKMMADATRDNVGKQMAVLFVEHKQRIKFTTDANGQTVEIREP
                     YVEKNVINRATVQSMLGSQFRITGLDSPEEGKELALLLRAGALAAPMYFVEERTIGPS
                     LGQDNIDKGLLSTQVGFAAVALFMLVFYRVFGIVANIALFMNVAMMIAIMSAIGAALS
                     LPGIAGIVLTVGMAVDANVLIFERIREELRAGQSPQSAIIAGYDRAYTTILDANITTL
                     IVAFILFAVGTGPVKGFAVTLSIGIMCSMFTAITVTRAVVQIIYGNRRVAKLSI"
BASE COUNT         2253 a         1411 c         1867 g         2432 t
ORIGIN      
        1 ttagaagcac aaattcatgg cggtggccat gagcgtggtt tccgttcggg gactttagcc
       61 acgcatcaaa tcgtgggtat gggtgaagca ttccgtatcg ccaaagaaga aggcatcgct
      121 gagcaacaac gtttagccgt gttgcgtgac aaattgtggg cgggcttatg ttctttagag
      181 caagtgtttt taaacggtga cgataagcag cgtattgctg gcaatgttaa cgtcagcttc
      241 aactttgttg aaggtgaatc attaattatg gcgttaaaag atattgctgt gtcatctggt
      301 tcggcctgta cttctgccag cttagagcca tcttatgtgt tgcgtgcctt gggattaagt
      361 gatgaattag ctcatagctc aattcgtttc accattggtc gtttcaccac agaagaagac
      421 attgattttg tcattaatca tgcccaaaac gcggtgaaca aactgcgcga cttatcacct
      481 ttatgggata tgtacaaaga aggcattgat ttaagtcaag ttcagtgggc agcacattaa
      541 tttttttcag aggatacagt catggcttat agtgaaaaag tgattgacca ttacgaaaat
      601 ccacgcaatg ttggtaactt agataaaaat gacactcatg tgggtacagg tatggtcggt
      661 gcgcctgctt gtggtgacgt tatgagatta caaattcgtg ttaatgatga tggtgtcatt
      721 gaagatgcac gttttaaaac ctatggctgt ggttctgcca ttgcttctag ttctttagtc
      781 accgaatggt taaaaggtaa aaccttagac caagcacagc aaatcaaaaa tgccgagatt
      841 gctgaagaat tagcattgcc tcctgtaaaa atccattgct ctgtattggc agaagatgcg
      901 attaaagcag cgattgaaga ttatcgtaaa aagcaaactg gtgaataagg agcagattgt
      961 ggctgtttca ttaactcctg ctgcggcaaa tcatgtgtca gattttatta ctaatcgtgg
     1021 caaaggcgag ggcattcgtt taggtgttaa ggtatctggc tgttcaggtc tcgcttatgt
     1081 tttagaattc gtagataatg cggattctgc cgaccaagtg tttgagtctc atggcgtaaa
     1141 aatctttgtt gacccaaaaa gcttggtata tttagacggt attgaaatgg actttgtcaa
     1201 agaaggtcta aatgaaggct ttaaatttag caaccccaac caaaaaggtg agtgtggttg
     1261 tggtgagtcg tttaccgttt aacagtgctt gttgtcatta atgatatgta gcccgtgtgt
     1321 cgtgcaacga aacacgggac ggtcttttga gtcctgtatt tctacaggct acacaagtct
     1381 gtctaataaa ttgaggttgt tgtggataag caagataatt actttgcgtg gtttgatttg
     1441 cctgttgcct ttcaaattaa tcaagacgca ttagcaagtc gttatcgaga gctacaacgt
     1501 caatatcatc cagatcgttt tgctaatcat cctgacgagc aacaaaaagc agtgcagtgg
     1561 tctgcacaac tgaatactgc ttatgacatc ctcaatagtg atgtgaaacg tgccagttac
     1621 ttattagagt tatctggccg tcctatctct ttaaatacca gtattgctga taccgacttt
     1681 ttaatgagcc aattagagtt gcgtgaacaa cttgatgaag cagaagtcgt tgagcaatta
     1741 gtgggtttac gtctcgaagt tgaagagtgg ctacaaagtt tatcgcgtga atttgtccta
     1801 gattatgccg atgaagattg ggttgaagcc caagatactg tccgcaaaat gcattttatg
     1861 gctaattttt tactcgatat tcgtcagcaa gaagataaat ttgaagacga tgactattat
     1921 gatgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnncctta tcaatttcgt
     2041 gctgaacaaa cggggatgcc tgcctttatt accgcacaag gtgaaaaaac gcctgttgaa
     2101 gtgtcggcag atattttgtc agtgttaaaa cagcgtgctg aagcggctct ggatggtgag
     2161 ttaaaagggg cggttattac cgtgccagcc tattttgatg aagctcagcg tcaagcaaca
     2221 cgtgatgcag ccacattagc aggtttaaaa gtattacgtt tattaagtga gccaactgcc
     2281 gctgcggttg cctatggttt agatcatggc gcagaagggg ttcatgccat ttttgatttg
     2341 ggtggcggta catttgatat ttctttgttg cgtttgcatc aaggcgtttt tgaggtatta
     2401 gccactggcg gcgatgcggc tttaggcggt gatgattttg atcgagcaat tgcgttgtgg
     2461 attatgcaac aagttgattt agcacaacct aatgcagcgc aacaacgtga gttaatggca
     2521 attgcttgcc aagccaaaga gcaattaacc acacaatcaa cgacaacaat cacatggcaa
     2581 acgtggcaag ctgaactcag tgtggtgcaa tttaatgagt taattacacc actggttgaa
     2641 aaaaccttac gtgcttgcaa gcgtgcctta cgtgatgcca atttaaaagc caatcaaatt
     2701 ttagaagtgg tgatggtggg tggctcaact cgagtgccgt tagtgcgcca aaaagttgct
     2761 gaattttttg gtcgtcaacc tttaaccaat attgatcctg ataaagttgt ggccattggt
     2821 gcagcaattc aagctgatgt gttggtgggt aataaacctg actctgatat gttgttattg
     2881 gatgtgattc ccttatcgtt gggtttagaa acgatggggg gcttggtaga aaaggttgtg
     2941 ccacggaaca ccacgattcc tgtgacgcgt gttcaagagt ttacgacctt taaagatggc
     3001 caaaccgcga tgagtattca tgtggtgcaa ggtgagcgcg aattagtaca agattgccgt
     3061 tcattagcac gttttgaatt acgtggcatt ccaccaatgg ccgcaggagc tgcccgtatt
     3121 cgcgtggtat ttcaagttga tgccgatggc ttattaaatg tcagtgccat tgaagtgagc
     3181 tcaggtcaaa aaagcgaaat taccgtaaag ccttcttatg gtctgcatga gcaagatatt
     3241 agtcgtatgc tcactgaatc ttatcaagcg gctgaggcag acaaacaagc cagagccttg
     3301 catgaagaaa aagtcgaaag tcagcaatta ttagaagcaa ttaaagcggc gttgcgtgaa
     3361 gatggtaaat tgttgtgtga aggtgaatta agcaccttac acgaaggcat taaacatctc
     3421 gaaattgctt tgggttcaac acaacgtcaa attattgaaa aagcacgttt agccttaatt
     3481 cctctatcgg atgcttttgc agcacgccgt atgaatcaac atattcacga tgccttaagt
     3541 ggccataaat taagcgactg ggagtaaaat atgcccaata ttattttttt accacatcaa
     3601 caattatgtc ctgatggggc agtggtagaa gccgaagtgg gtgaaactat ttgtgatgct
     3661 gctttgcgtg ctggtttaga aattgaacat gcttgcgaaa agtcttgtgc ctgtacaacc
     3721 tgtcatgtgg tggtgcgtga aggttttgat agcttaaatg aaatggatga cttagaagcg
     3781 gatatgctgg ataaagcatg gggtttagaa ccagactccc gtttatcttg tcaggcagtg
     3841 gttgctgatg aagatttggt ggtggaaatt cctaaataca ccattaatca tgcgtctgaa
     3901 aaacattaat ttttattccc tcccctaatt tagggggagg agtttaattg gagagtaaca
     3961 tgagtttaaa gtggacagat tctcgtgaaa ttgctatcgc gttgttcgat gctcatcctg
     4021 atgttgaccc aaaaaccatt cgttttaccg atttgtataa ttgggttatc gctttaccag
     4081 aatttgctga cgatccaaaa cacagtggcg aaaagatttt agaagccatt caaatgtgtt
     4141 ggttggaaga agctgattaa gttaaattga tttaggactg acgccgttat tactggatta
     4201 tgcacattta attgttttta tgctcataaa gtgtgctaat ttggttgaaa tagtgaagtc
     4261 ctgatattaa ttttggcaaa attcctccgc cctgagtttt tatccctcct aattaggcta
     4321 taatttcgcc cctgtcaaaa ttacgttcaa aagatgacgt actcattaat cctgttgaac
     4381 gatgacacaa cgcattaact gcgattctgg tcttggtgtt ttttaattat gcaatttaaa
     4441 cttttagcca ctgatggtat tgcccgccgc ggcgagctca ccttacaaca tggtaaggta
     4501 caaacgcctg catttatgcc tgttggtaca tacggtactg ttaaaggcat gtttcctagt
     4561 gatatcgaaa atattggtgc agatattatt ttaggcaata cctttcattt gtggttaaga
     4621 ccaacgacag aagtcgtcaa acagtttggc ggtttgcata agtttattaa ttgggacaaa
     4681 cctattctta ccgattcggg tggctttcaa gtatttagct tgggggcatt gcgtaaaatc
     4741 agtgaagaag gcgttaagtt tcaatcgccg attgatggtt caaaagtatt tttaaccccc
     4801 gaagtttcca tgcaaattca acgcgagtta aattctgaca tcgtgatgat ttttgatgaa
     4861 tgcacacctt atcctgccac cgaaaaagaa gcgcgtgact cgatggagtt aagtttgcgt
     4921 tgggcagcac gttgtaaaac cgctcatgaa ggcaatccca atgctttatt tggtattgtt
     4981 caaggcggga tgtatgagca tttgcgtgat gtgtccttag ctggattatt agatattggt
     5041 tttgatggtt atgccattgg tggtttatcg gtgggtgagc ctaaagatga aatgttgaaa
     5101 attctcaacc atgtccccac taaattacct aatgataagc cacgttattt aatgggcgtt
     5161 ggtaagcctg aagatattgt tgaagcggta agacgtggcg tggatatgtt tgattgtgtc
     5221 atgccaaccc gtaatgcgcg taatggtcat tattttgtca ccaatggtgt ggtaagaatt
     5281 cgcaatagtc agtacaaaac cgatcaatcc ccgattgatg aacattgtga ttgttatgct
     5341 tgcaagaact tttcacgcgc ctatttacat catttagaca aatgtggcga gatgttgggc
     5401 gcaatgttag gcacactaca caacctgcgt tattaccaac gattaatggc tggtttacgc
     5461 caagcgattg agcaaggtca actacaagcc tttgttgccg acttttatgc tcgtcgtgaa
     5521 ttgcctgttc ctagtttgta atttttaggc ttacgcattg cactgcaatg agcgtgtttg
     5581 ttgagcatga agtcgttgaa agcgactgaa gtgcgaacca ataaacgcta accgcgtagg
     5641 ccttataaag agagagagaa acatgagttt ttttattgct gaagcctatg cggctgcccc
     5701 tgctgctcaa gaaccttctg cgattgccaa tattgtcatg ttggttgttt ttatgttggt
     5761 gttttacttt ttattatggc gtccacaatc caaacgccaa aaagaacacc aagccttaat
     5821 gactggctta caaaaaggcg atgaagtcgt ctttgctggc ggcttattag gtaaaattaa
     5881 aagtgttgat gaagattatg ctgttgttga aattaacgat aatttaaccg ttaaaattca
     5941 aaaagcatcg gtattagcga cattacccac aggaacttta aaagggattg gtacaggctc
     6001 tagcaccaat ttaagtaaag attaaaaaat atagacttgt aaacaatagg aataagtttg
     6061 caagtcatca ttgatgaaag aataattatg cgttaccccc tttggaaata tattttaatt
     6121 gttattgtgc tcttggtgag cgcaatttat tccttaccta atttatatcc tgatgagcct
     6181 gctatccaaa tttcgggggc taatgcaggt gttaaagcag ataaaacagt attgagccaa
     6241 gcagagacag ctttaaaaca agcaggttta acgtttcatg gttcagaagt gcaagccaaa
     6301 ggggtgttat tacgtgttaa caccagtgat gagcaattaa aagcgcaaga aattgtccgt
     6361 cgtgctttgg gtgatggtta tgttgttgca ctaaatttag caccaaccac accagaatgg
     6421 ttattaagcc tcaacgctgc cccaatgaag ttgggtttgg atttacgggg tggtgtacat
     6481 ttcttattag aagttgatat gagtaaagtc ttggagcaac gccaagaaac ttatgcaact
     6541 gacatcaaag ccaaattccg tgaagcaaaa gttgcttatc gtacggtagc tgatattact
     6601 aatggtttaa ccgtcaagtt tgccaaagaa gaagatcgta ctgctgctaa agagttattg
     6661 gcacgcgaat atcgtgaatt tatcttagtg ccagcaatgc gagataacga actgtattta
     6721 gatttagcgt ttgcgccaga aaaattaaaa gaattttctg attatgcgat tgaacaaaat
     6781 ttaacgactt tgcgtaaccg agttaatgag cttggtgttg ccgaagctgt ggtacaacgt
     6841 caagggtcta atcgtattgt tgtacagtta cctggtgtgc aagatacagc cgaagccaaa
     6901 cgtgttttag gtcgtactgc aaccttagag tttcgttttg ttgattggga gcatcaaggt
     6961 gcagttgatg gcatagcacc tgcaggcaca gaaattttcc caatgaataa aaccaaagag
     7021 ccagttttgt tgcaacgtaa aaaaattgtc acaggcgaca gagtggtaaa tgctcaatca
     7081 ggatttgatg aatattctaa tccacaagtt aatatcacct tagatggtcg tggcggcaaa
     7141 atgatggctg atgccactcg cgataacgtt ggcaaacaaa tggctgtatt atttgttgag
     7201 cataaacaac gaatcaaatt taccaccgat gcaaatggtc aaacggttga aattcgcgaa
     7261 ccttatgttg aaaagaacgt cattaatcgt gcaactgtac aatcaatgtt aggctcacaa
     7321 ttccgtatta ctggtttgga ttcaccagaa gagggtaaag agctagcctt gttattaaga
     7381 gcaggtgcat tagctgcgcc catgtatttt gttgaagaac gcaccattgg tccaagttta
     7441 ggccaagaca atattgacaa aggtttgtta tctactcaag tgggttttgc agcggttgcc
     7501 ttgtttatgt tggtgtttta tcgcgttttt ggcattgtgg ctaatattgc cttgtttatg
     7561 aacgttgcga tgatgattgc tattatgtct gccattggtg cggcattaag tttaccaggt
     7621 attgcaggta tcgtattaac agtaggtatg gcggttgatg ctaacgtatt gatttttgaa
     7681 aggattcgag aagagttgcg agcaggccaa tcaccacaaa gtgcaattat tgcaggttat
     7741 gatcgtgcat ataccaccat tttggatgca aacattacaa cgttaattgt cgcatttatc
     7801 ttatttgctg taggtacagg gccagttaaa ggttttgctg tgactttatc gattggtatt
     7861 atgtgctcta tgtttactgc cattacggtg acacgtgcag tggtgcaaat tatttatggc
     7921 aaccgtcgtg ttgccaaatt gagcatttaa ggggaacgca tcgtggaaga aaaaattatt
     7981 cgctttatgc gttttcgcaa accgatggct attttttcct ttttagtggt attaattggc
     8041 gttatttcta tttttaccaa agg
//
LOCUS       DAOQMB010000064         7926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3680_length_7926_cov_14.295769, whole genome shotgun sequence.
ACCESSION   DAOQMB010000064 DAOQMB010000000
VERSION     DAOQMB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3680_length_7926_cov_14.295769"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..543
                     /gene="coaD"
                     /locus_tag="PLY05_03765"
     CDS             58..543
                     /gene="coaD"
                     /locus_tag="PLY05_03765"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNC02452.1"
                     /translation="MTKTRVLYPGTFDPITNGHIDLVERAARLFDEVVVAVAAGHHKK
                     PLFDLDERVQLVQQVTAHLPNVTVCGFSQLLAKFVQEQQATAVLRGLRAVSDFEYEFQ
                     LANMNRQLFVDFETVFLTPSENLSYISSTLVREIARLGGDVSKFVAPQVVEAFNKKFG
                     G"
     gene            547..798
                     /locus_tag="PLY05_03770"
     CDS             547..798
                     /locus_tag="PLY05_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HNC02453.1"
                     /translation="MALMITDECINCDVCEPVCPNDAIFQGPDIYEIDAGLCTECVGH
                     FDEPQCQTVCPVSCIPLDPRHVETKDQLLAKLQRNQGQA"
     gene            complement(944..1294)
                     /locus_tag="PLY05_03775"
     CDS             complement(944..1294)
                     /locus_tag="PLY05_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02454.1"
                     /translation="MSLFSRRALANCALILSCAIATPSFAEEYMDDQVNQRPGAGAMV
                     LDAAVARPMLLALTLGGTAAFVASLPFTALGGNVEEAAKTLVVGPAKATFTRCMGCTD
                     TQDRLKNRDIANNQ"
     assembly_gap    1573..1672
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1673..1971)
                     /locus_tag="PLY05_03780"
     CDS             complement(<1673..1971)
                     /locus_tag="PLY05_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HNC02455.1"
                     /translation="MPELPEVETTKQGLLPLLNHKVAAIHIRQPRLRQLVPDSLHDMI
                     GQTCLEFSRRGKYIILKTESGHALIHLGMSGSLRLCSPTEDYRKHDHVILNFSNG"
     gene            complement(2073..2228)
                     /gene="rpmG"
                     /locus_tag="PLY05_03785"
     CDS             complement(2073..2228)
                     /gene="rpmG"
                     /locus_tag="PLY05_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNC02456.1"
                     /translation="MRDKIRLVSSAGTGYFYTTTKNKRTMPEKMEIKKFDPKIRQHVI
                     FKEAKIK"
     gene            complement(2239..2475)
                     /gene="rpmB"
                     /locus_tag="PLY05_03790"
     CDS             complement(2239..2475)
                     /gene="rpmB"
                     /locus_tag="PLY05_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNC02457.1"
                     /translation="MSKVCLITGKRPIVGNNISHANNKTKRRFEPNLHYHRFWLESEK
                     RFVRLRVSSKGMRTIDKLGVEQVVADLRANGHKI"
     gene            complement(2580..>2813)
                     /locus_tag="PLY05_03795"
     CDS             complement(2580..>2813)
                     /locus_tag="PLY05_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006580455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC02458.1"
                     /translation="QSGGFSANDVITHVAEEDLPFGGVGHSGMGKYHGKEGFAAMSNA
                     RSVLVKPKLWSIRFLAPPFKNPIHKFIEKVLLK"
     assembly_gap    2814..2913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2914..3800)
                     /locus_tag="PLY05_03800"
     CDS             complement(<2914..3800)
                     /locus_tag="PLY05_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC02459.1"
                     /translation="MQRVFDAQKTAFRLRPSTTATERLALLDNLKTVLIKYQDQIAQA
                     IAEDFGHRSHHETKFAEILTSIENIKYYSKNLRNWMQPDKRHINPTHLPAKAWVQYQP
                     LGVVGIITPWNYPLLLAISPLICALAAGNRAMIKMSSFTPKTGAIVKKALTEAFNEDQ
                     VAVITGGGIVSDAFSRLPFNQITFTGSTNVGRTVMAAAAENLTPVLLELGGKSPAIIH
                     PSVSMKDAAEKLAFGKGWNAGQTCVAPDYVFVPRGKVAELAAAMRVVVAKMYPKMLNN
                     PDYTSIVNNKQYQRVKSYL"
     gene            4223..4876
                     /locus_tag="PLY05_03805"
     CDS             4223..4876
                     /locus_tag="PLY05_03805"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02460.1"
                     /translation="MKTRDRILLKSLELFNEQGERTITTNHIASALNISPGNLYYYFR
                     SKEDIIFEIFLRYEQSMLNALAVPTDRNVTYMDKIGYFEAIFHQLWEYRFLHRDLAHL
                     LEESPRLRASYRSFAKKVMAQAYLVYNALREAKFIQATEQDVRAMIVNIWILTTSWAS
                     FVVSSGFFGEEVVLSPQMLRRGIYQIICLEAPYLQGEALARLPDLLQEYRQAAEIVD"
     gene            5078..7699
                     /locus_tag="PLY05_03810"
     CDS             5078..7699
                     /locus_tag="PLY05_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02461.1"
                     /translation="MHNWEHYGRLPAYLLAATLVASCGGGDGDLLTDTNGVSNAKVTL
                     APTVGGDAVQLTNVGESSFTVKLEGADKKPIANKPVTLSVVGKGISLSSNSLITAADG
                     TLLVTVKGDGAGSIGDTGVIKAVYKDEKGDIAEQQLIYKITSVSELKTSNALTGCFKQ
                     GSTCTSALATLTVSGIGNSALAQFTLTDKDNKALVEKDVVFKLANANGALTTTKGRTD
                     ANGKVEVQISSASTASSNSVSAVYTDANGVETVGTLNFKTIVGNRVSLSANKSELISG
                     GDAVDLTAVVISPTGNVQANVPVSFVLANPSEKGVSIQSTSPVTDANGLAKATLKISN
                     VNGANLANRDDFKVKAIIGTGASEDEVNELSIPVVGTNLALSTEKISVKTNDKITIKG
                     VLKDGTGKSITGSQTVTLVSEGLSGVPASLVISNGQFELKDIVVASTGTTAKIAASAL
                     GADQSLSFDISTSSFDVDAPEKIDIDKGGVINFAYSVADGTSTTPINMTVSSTLGNIP
                     SPVILRTDDGGKTFKASVSITSQFPGKAVVRAVFKDSANKEIVANKTITYVSTTPAKL
                     AIQAVSPTLVPNGQTNIIAKVTDANDNPVSDAVVNFKLENPLGSELDNPSVKTNDKGE
                     AIVSFKAGSGTTGTEKITVKADVLDEKSGVTKTSSLNLTIGGKAVFISIATGNQIEEL
                     TTTTYAVPHQITVTDATGAPVANTEVKISVWPVNYYKGTYVFNTEKKLWIPIYSAECS
                     NEDANQNGFLDLWENNGTGVALSPFDYPKGEETDVEDNKDGKLWPGNPVTLSTSTLTT
                     GADGIAYFNVLYGQSYANWLKVKLTAKAQVQGTESKTDRVFGIPASSGDMGDEKVSPP
                     GGTQSVYGTATVCTDPN"
BASE COUNT         2400 a         1301 c         1625 g         2400 t
ORIGIN      
        1 aatcaaagga cttaagttct tgacaactta agtcctattt ttttggagct ttttaacatg
       61 actaaaaccc gtgtactcta tcccggtacg tttgacccaa ttactaatgg ccatatagac
      121 cttgtcgaaa gagccgcacg tttatttgac gaagttgttg ttgctgttgc cgcaggccac
      181 cataaaaaac ctttatttga tttagatgaa cgagtacaac tggttcaaca agtaacagca
      241 catttaccaa atgttacggt gtgcggtttt agtcaattat tagccaaatt tgttcaagag
      301 caacaagcaa ctgcggtatt gcgtggttta agagccgttt ctgactttga atatgaattt
      361 caactagcca atatgaatcg acaattattt gttgattttg aaacagtatt cttaacgcct
      421 tcagaaaact tatcgtatat ttcatcaaca ttagttcgtg aaattgctcg attaggtggt
      481 gatgtctcta aatttgttgc ccctcaagtg gttgaagctt ttaataaaaa gtttggaggc
      541 tagtacatgg ccttaatgat taccgatgaa tgtattaatt gtgatgtgtg tgaacctgtt
      601 tgtccgaatg atgctatttt tcaagggcca gacatttatg aaatagatgc aggtttgtgt
      661 actgagtgtg ttggccattt tgatgagcca caatgccaaa cggtttgtcc tgttagctgt
      721 attccgcttg acccacgtca tgtggaaaca aaagatcaat tgttagctaa attacaaaga
      781 aatcaaggac aggcttagat ttagagaaaa tttaggcata aaaaaacccc accaacaagt
      841 aggattggcg gggtgaaagt ggattaaatc ttttaatcct gcgcgtattc tatgcaaatt
      901 ttaaatgaaa atctacaaga agttgccaat ttaaagtaaa aaattattga ttattggcaa
      961 tgtcgcgatt ttttaagcgg tcttgtgtat cggtacaacc catacagcgc gtaaaagttg
     1021 ctttggctgg gccaacaact aaggtttttg cggcttcttc aacattgcca cctaatgctg
     1081 taaaaggtaa agatgcaaca aatgctgctg ttccacctaa agtgagagct aataacattg
     1141 gacgtgcgac tgcggcatct aataccattg cccctgcacc tggtctttga ttgacttggt
     1201 catccatgta ttcttcagca aaagaaggag tggcgatcgc gcaagataaa attaatgcac
     1261 aattagctaa agcgcgacgt gaaaacagac tcattattat ctccttgtaa aaggggtagc
     1321 gtgttagata cgctcttcat tgtaataatt aaaaaataat agtatcgaat ctaatccgag
     1381 ttgtgtttag atgcaagccc taagcacaaa aagctggctg acattgtgaa cagtatatcg
     1441 tacttcgatt atttatgcgt attaattgta gcgggctgtg acaaataata cacggttttc
     1501 cttcttttcc atatacagat aactcttgtt gaaaatagcc catttcgcct tgactgttga
     1561 caaagtcgcg tannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaccatt
     1681 actaaagttt aaaattacat gatcatgttt acgatagtct tccgttggag aacataaacg
     1741 taaactccca gacatcccta aatgaatcag agcatggcca ctctcagttt ttaaaataat
     1801 atatttgcct cgacgactga attcaagaca ggtttgacca atcatgtcat gtaagctatc
     1861 agggactaat tggcgtaaac gtggctgtcg aatatgaata gcagcaacct tatgattaag
     1921 aagaggtagt aaaccttgtt tggtagtttc gacttcagga agttctggca tgatgaatta
     1981 atgtcattga aaagaaacag agtggctgag cgatgtcgaa gccagaaaag caaaaagccc
     2041 gataaatcgg gctttttaaa gcaaaatcaa aattacttga ttttagcttc tttaaaaata
     2101 acgtgctgac gaatctttgg atcaaatttt ttgatttcca ttttttcggg cattgtacgt
     2161 ttatttttag tggtggtgta aaagtaacct gtaccagcag aggatactaa gcgaattttg
     2221 tcacgcatga tttaaacctc agattttgtg gccattagcg cgcaagtcag caacaacttg
     2281 ttctacgccg agcttgtcaa tagtacgcat acctttagaa gatacacgga gacgcacaaa
     2341 acgcttttcg ctttctaacc aaaagcgatg gtaatgcaaa ttaggttcaa aacgacgttt
     2401 tgttttgttg ttagcatgcg aaatgttgtt gccaacaata ggacgcttgc cagtaattaa
     2461 gcaaacttta gacataaaat actcccaaat gcgatcagaa ccgccgcctt aacgacggca
     2521 gacagcaagg ggcgcattta taacaaaatc gccccttgac agcaagagtt cttgagaaat
     2581 tattttaata aaactttctc aataaacttg tgaattggat ttttgaaggg tggggctaag
     2641 aaacggatag accacaactt aggtttcact aatacagagc gtgcattaga catggcggca
     2701 aaaccttctt tgccatgata tttacccatg cctgagtgac caacaccacc aaacggtaaa
     2761 tcttcttcgg caacgtgggt aatgacgtcg ttggcagaga aaccaccaga ttgnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntctaaat agctttttac ccgttgatat
     2941 tgcttattat taacaatgct agtgtaatca ggattattaa gcattttagg atacatttta
     3001 gccacaacaa cacgcatggc tgcggctaat tcagcaactt ttcctcttgg cacaaataca
     3061 taatcaggag caacacaggt ttgtcctgca ttccaacctt taccaaatgc taatttttca
     3121 gcagcatctt tcatactcac agaagggtga ataattgcag gagatttgcc acctaattcc
     3181 aaaagaacgg gtgttaaatt ttcagcagcg gctgccatga ctgtacggcc aacattggtt
     3241 gaacccgtaa aggtaatttg attaaaaggt aaacggctaa atgcgtcaga tacgatacca
     3301 ccacctgtaa tcacagcaac ttgatcttcg ttaaatgctt ctgttaaagc ctttttaaca
     3361 attgcaccag tttttggggt aaaactggac attttaatca ttgcacggtt gcctgcggcc
     3421 aatgcacaaa ttaatgggct gattgctaat agcaaaggat agttccacgg cgtaataata
     3481 cctacaacac ctaatggttg atattgtacc caagccttag caggtaagtg cgtagggtta
     3541 atatggcgtt tatctggctg catccaattg cgcaaatttt tgctgtagta tttgatattt
     3601 tcgatgctag ttaagatttc ggcaaacttg gtttcgtgat gtgagcgatg accaaagtct
     3661 tcagcaatgg cttgagcaat ttgatcttgg tatttaatta atactgtttt caaattatct
     3721 aacaatgcaa ggcgctcggt tgctgttgta ctaggtctta aacgaaaggc tgttttttgt
     3781 gcatcaaata cgcgttgcat atcagcaact tcaggatgat cggtatgcag aggtgaaaca
     3841 ctggcggtca tattaaagcc tctaaatatc aaagggctga gtgagtataa agttaacaat
     3901 acagaagtag tggtactgtc gtgctaatat ttgactattt ttagagtatt tgctctaatt
     3961 tgtcaaatga aaattaaatt tttccgctga atggttagag agacgttttg ataacgactg
     4021 ctctagcagt gaaaagtggt atttcgtgtg gttgaattga gtgatttttg tattattgag
     4081 cataataaac agaaaaactt aacaaaatat gccaattttt aagtgtgatg ctgttaatat
     4141 tttaatagtt ttagcggtat cataaatgat atctgtagca atagaacaat accaatgaag
     4201 ttgctataac gaggaaagcc ctttgaaaac acgtgatcgg attttattaa aaagcttaga
     4261 gttatttaat gagcagggtg agcgtaccat cacaactaat catattgcat cagccttaaa
     4321 tataagtcca ggtaatctgt actactattt tcgtagtaaa gaagacatta tttttgaaat
     4381 ttttttacgt tatgaacaaa gcatgttgaa tgccttagct gttccaactg accgtaatgt
     4441 cacctatatg gacaaaattg gttattttga agcgattttt catcagttgt gggagtatcg
     4501 ctttttacat cgagatttgg ctcatttatt agaggaaagc cctcgtttaa gggcttcata
     4561 tagaagcttc gcaaaaaaag tgatggctca ggcttatttg gtttataatg ctttgcgaga
     4621 ggcgaaattt attcaagcga cagagcaaga tgttcgtgcg atgattgtta atatttggat
     4681 tttaacaaca agttgggcat cttttgtagt aagtagtggc ttttttggtg aagaggttgt
     4741 tttaagtcca caaatgttgc gtagaggtat ttaccaaatt atttgtttgg aagcacctta
     4801 tttgcaaggt gaggctttag caaggctgcc tgatctttta caagagtatc gtcaggctgc
     4861 tgaaattgtt gattagctca aaaaatacaa agagtttaac aaaaaataag aaagacttac
     4921 ttcacacaat ctatctacaa tacgtttaaa tcgttgaagt tttttacctt atttagtata
     4981 agtgaaatat ggtgcgtctt tacgtctttg agtgctacag tatgttcgat ttaataggat
     5041 gtagcacaat agcaacaaca aacgagggtt tataacaatg cacaactggg agcattatgg
     5101 ccgtttgcca gcataccttt tagcagcaac tttggttgca tcctgtggtg gtggagatgg
     5161 ggatttatta acagatacca atggtgtatc gaatgctaaa gtaactttag ctcctacagt
     5221 gggtggtgat gccgttcaat taacaaatgt tggcgaaagt agttttacag tcaagcttga
     5281 aggtgcagat aaaaagccaa ttgctaataa gcctgtaact ttaagtgttg ttggtaaagg
     5341 tatatctcta tcttctaatt cattgataac agcggctgac ggtactcttt tggttactgt
     5401 taaaggtgat ggtgctggta gtattggtga tactggtgta atcaaagcag tttacaaaga
     5461 cgaaaagggc gatattgcag aacagcagct gatctataaa atcacgagtg tttcggagtt
     5521 aaaaacttct aatgcgctaa caggatgttt taagcagggt agtacttgta cttctgcctt
     5581 agcaacgttg acggtgtctg gtattggtaa ttctgcgtta gcacaattta cattgactga
     5641 taaagacaat aaagctcttg tagaaaaaga tgtagtcttt aaattggcta atgctaacgg
     5701 tgctttaacc acaactaagg gacgcactga tgcaaatggt aaggtagaag ttcaaatttc
     5761 atcggcatca acggcatctt caaatagcgt gagtgctgtc tatactgacg caaatggcgt
     5821 tgaaactgta ggcactctaa actttaaaac gattgttggc aatagggtta gtttaagtgc
     5881 taataaatct gaattaataa gcggtggaga tgcggttgat ctaacggctg tcgttatttc
     5941 tccaacaggt aatgtacagg ctaatgtgcc tgtttctttt gtgttggcta atccaagtga
     6001 aaaaggtgta tcaatacaat ctactagtcc cgtgaccgat gcgaatggtt tagctaaagc
     6061 aaccttaaaa attagtaatg ttaatggtgc caatttggca aaccgagatg attttaaggt
     6121 taaggcaatt atcggcacag gtgcaagtga agatgaggtt aatgagttat ctattcctgt
     6181 tgtaggtact aatttagcat taagcactga aaagataagt gttaaaacga atgataaaat
     6241 tactattaaa ggtgttttaa aagacggaac tggtaaatct attacaggct ctcaaactgt
     6301 tactttagtt tctgagggat tgtctggtgt accagctagc cttgttatta gtaatggtca
     6361 gtttgaactt aaggatatag ttgttgcttc aacgggtaca accgccaaaa ttgcagcttc
     6421 tgctttaggg gcagatcaga gtttaagctt tgatatttct acaagtagtt ttgatgttga
     6481 tgctcctgaa aaaattgata ttgataaagg tggagttatc aattttgctt attctgttgc
     6541 tgatggtaca tcaacaacgc caatcaatat gacagtgtca tcgacattgg gaaatatacc
     6601 atctcccgtt attttacgta ccgatgatgg tggtaaaaca tttaaagcga gtgtctctat
     6661 tacttcacaa tttcctggta aagctgtggt tcgcgctgta ttcaaggact cagctaataa
     6721 agagattgtt gctaataaaa cgattactta tgtctctact actcctgcta agttagcgat
     6781 tcaggctgtg tcaccaacat tagtacctaa tggtcaaact aatattatag ctaaggtaac
     6841 agatgcgaac gataacccag tttctgatgc tgtagtaaat tttaaattgg aaaatccttt
     6901 aggctcggaa ttagataatc cgagtgttaa aactaatgac aaaggtgagg caatagtctc
     6961 ttttaaagcg ggttctggta caacaggtac agaaaaaatt actgttaaag ctgatgtttt
     7021 agatgaaaag tctggagtaa ctaagacaag ttcattaaat ttaaccatag gtggtaaagc
     7081 agtatttata tctattgcaa caggaaatca gattgaagag ttaacgacta cgacttatgc
     7141 ggttcctcat caaattacgg ttacagatgc gacaggcgca cctgttgcta atacagaagt
     7201 aaaaatttca gtatggccag ttaactacta taaaggaacc tatgttttta ataccgaaaa
     7261 gaaactttgg atacctattt atagtgctga atgtagtaac gaagatgcaa accaaaatgg
     7321 cttcttggat ttgtgggaaa ataacggcac aggtgtggct ttaagtccat ttgattatcc
     7381 taaaggcgaa gaaacagatg ttgaggataa taaagatggt aaattatggc ctggtaaccc
     7441 tgtgacttta tctacgagta cattaactac aggtgctgat ggtattgctt attttaatgt
     7501 gttatatggt caaagctatg ctaactggtt gaaggttaag ttaactgcaa aagcacaagt
     7561 tcagggtaca gagtcaaaaa cggatagagt ctttggtatt cctgcaagct ctggcgatat
     7621 gggggatgaa aaagtttctc cacctggtgg gacacagagt gtttatggta cagcaactgt
     7681 ttgtactgat ccaaactaag tcatattttt taatatgaaa gaacgcgcag ccccaataag
     7741 tctgcgcgtt ttttttggta atgagtgcta ttttgtttaa agttcgctat atttagaatg
     7801 ttgcgctcta aaagctaatt caataattta taaaaatcag tagcaggtat ttattatgca
     7861 aggccatatt tctggaactt atgtgcgcct gttgtttgaa tggctcaatg ataaaggctt
     7921 tgaggc
//
LOCUS       DAOQMB010000065         7777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3776_length_7777_cov_16.018778, whole genome shotgun sequence.
ACCESSION   DAOQMB010000065 DAOQMB010000000
VERSION     DAOQMB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3776_length_7777_cov_16.018778"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /gene="glmU"
                     /locus_tag="PLY05_03815"
     CDS             complement(<1..1055)
                     /gene="glmU"
                     /locus_tag="PLY05_03815"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HNC02462.1"
                     /translation="MELSVIILAAGKGTRMKSVLPKVMQPLAGKPLLAHVLATAQELQ
                     SSRTIVVYGHGGEVVQKHFAHQENLAWALQAEQKGTGHAVQMALSQLPETGKSLILYG
                     DVPLTTPETLQKLLDLVVDNQKIALLTVNLANPTGYGRIVREAGLVQRIVEQKDASEQ
                     EKQITEVNTGILCVPNCHLHQWLPTLKNNNAQGEYYLTDVIALAVQQGLTVHTYQPQA
                     AWEVEGINDKLQLSTLERVYQRLQADNLMRAGATIIDPARFDMRGTATIGMDVFFDAN
                     VLLEGDNHFGNGVKVGTNCVIKNCKIADGVEIRANSVLEDAEIGQNAQIGPFARIRPQ
                     SKIGANVHIGNFVETKNT"
     gene            complement(1147..>1407)
                     /locus_tag="PLY05_03820"
     CDS             complement(1147..>1407)
                     /locus_tag="PLY05_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HNC02463.1"
                     /translation="IKEGGQEEVFYVSGGVLEVQPHIVTVLADAVVRADGIDEAAAIK
                     AREDALAALQNQKSEMDTGAALAALAEAAGQLRTLQQLKNRA"
     assembly_gap    1408..1507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1508..2826)
                     /gene="atpD"
                     /locus_tag="PLY05_03825"
     CDS             complement(<1508..2826)
                     /gene="atpD"
                     /locus_tag="PLY05_03825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNC02464.1"
                     /translation="MSSGQIVQIIGAVIDVEFPRDSVPKVYDALKVDGTATTLEVQQQ
                     LGDGIVRTIAMGSTEGLKRGLPVSNTNAPISVPVGTATLGRIMDVLGNPIDEAGPIGE
                     QAQWSIHRKAPSYDEQSGATELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSGVVGKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDESGKGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGVLQERITSTKSGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPLVVGEEHYTVARGVQTVLQRYKELKDIIAILGMDELSEEDK
                     LVVYRARKIQRFLSQPFHVAEVFTGSPGKYVSLKETIRGFKGILAGE"
     gene            complement(2888..3757)
                     /gene="atpG"
                     /locus_tag="PLY05_03830"
     CDS             complement(2888..3757)
                     /gene="atpG"
                     /locus_tag="PLY05_03830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HNC02465.1"
                     /translation="MAGLKEIRSKVVSIKSTQKITKAMQLVAASKMRRAQDRMAQGRP
                     YADKMRRVIAHLVQANPEYKHAYMVDRPVKRVGYIVVSSDRGLCGGLNINLFKRTVQS
                     VKTWREQGVEAEFCLIGQKATTFFKSVGGKVVAVKTQLGDAPSLDQLIGAVKVMLDAF
                     DRGEIDRIYLVSNQFVNTMTQKPVVNQLVPLSADNGDEPLKREHSWDYLYEPEAAPLL
                     DALMVRFIESQVYQGVVENIACEQAARMVAMKAATDNAGELIRSLQLVYNKLRQAAIT
                     REISEIVGGAAAV"
     gene            complement(3828..>4670)
                     /locus_tag="PLY05_03835"
     CDS             complement(3828..>4670)
                     /locus_tag="PLY05_03835"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997344.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC02466.1"
                     /translation="MQFLAPYSGCTMGEYFRDRGEDALIIYDDLSKQAVAYRQISLLL
                     RRPPGREAYPGDVFYLHSRLLERASRVSEEYVEKFTNGAVKGQTGSLTALPIIETQAG
                     DVSAFVPTNVISITDGQIFLETSLFNSGIRPAVNAGISVSRVGGAAQTKIIKKLSGGI
                     RTSLAQYRELAAFAQFASDLDEATRKQLEHGQRVTELMKQKQYAPLSIADMALSIFAA
                     NEGYLVDVPVAKIGAFEAALHGYMNSQQKALMDNINATANYDKDIEASLHNGIKAFKA
                     TQSW"
     assembly_gap    4671..4770
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4771..5027)
                     /locus_tag="PLY05_03840"
     CDS             complement(<4771..5027)
                     /locus_tag="PLY05_03840"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997344.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNC02467.1"
                     /translation="MQQLNPSEISSLIKQRISDLNATAEARNEGTVVKVSDGIVRIHG
                     LADVMYGEMIEFDGGIYGMALNLEQDSVGAVVLGDYLGLQEG"
     gene            complement(5048..5590)
                     /locus_tag="PLY05_03845"
     CDS             complement(5048..5590)
                     /locus_tag="PLY05_03845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HNC02468.1"
                     /translation="MAELTTVARPYAKAAFEYANAEGKLELAIWSTQLALAAAIVQDA
                     DFARYLSRPSMTVAEQETALFAACGGELSAGVRNFLSLVAANKRLMALPAIAELYEVL
                     KAQAENTADVTVTSAFALSDEQEDALAKQLAEKLGARINISTEVDAALIGGVIIRTGD
                     LVIDASVRGKLAKLSATLNT"
     gene            complement(5610..6080)
                     /locus_tag="PLY05_03850"
     CDS             complement(5610..6080)
                     /locus_tag="PLY05_03850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNC02469.1"
                     /translation="MNINATLLGQAISFAIFVWFCMKFVWPPLTAALAERQKKIADGL
                     NAATKAQDDLKAAQDQVAQELKAAKDQAAQLIEQANRRAGQLVEEAKSQAVVEGERIK
                     AQAREQIEQETNRARDQLRAQVAALAVAGAEKILQAEVNANAHAAMLSKLAAEL"
     gene            complement(6121..6378)
                     /gene="atpE"
                     /locus_tag="PLY05_03855"
     CDS             complement(6121..6378)
                     /gene="atpE"
                     /locus_tag="PLY05_03855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003097235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HNC02470.1"
                     /translation="METVIGLTAIAAAILIAFGALGTAVGFGLLGGKFLEGVARQPEL
                     APQLQVKMFIVAGLLDAVPMIGVGIGLFFVFANPFIGLVPA"
     gene            complement(6420..7253)
                     /gene="atpB"
                     /locus_tag="PLY05_03860"
     CDS             complement(6420..7253)
                     /gene="atpB"
                     /locus_tag="PLY05_03860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNC02471.1"
                     /translation="MAGSSTEYIKHHLTNLTFGNHPEHGWSFAHTAEEAQEMGFWAFH
                     VDSLGWSIALGLIFCLVFRSVAKKANSGVPSGVQNFVEMIVEFVDSNVKDTYHGKSSL
                     IAPLALTIFCWVFLMNLMDLIPVDFIPVVAQALGLHYMKVVPSTDPNITLGMSISVFM
                     LILFYSIKMKGVGGFAGELALNPFNPKNPILKAFFIPINLILEGVTLIAKPVSLGLRL
                     FGNMYAGELIFILIALLPFGVQWLLSVPWAIFHILVITLQAFIFMMLTIVYLSMASEH
                     H"
     gene            complement(7261..7656)
                     /locus_tag="PLY05_03865"
     CDS             complement(7261..7656)
                     /locus_tag="PLY05_03865"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HNC02472.1"
                     /translation="MSKPQPLVDKTPAIRLLKGQVLATTAITLIALIWGVWSSVSAAT
                     GASIALIGSAYFALQAFRHAGAGSAKQIVKSFYKGAAGRFGITVLLFSVAFAGLKSLQ
                     VGWVLAGFFIVQLVAWVSPLWDALQDERK"
BASE COUNT         2251 a         1810 c         1456 g         2060 t
ORIGIN      
        1 gtatttttgg tttcaacaaa attaccaata tgaacgtttg ctccaatttt actttgtgga
       61 cgaatacgag caaatggacc aatttgagca ttttggccaa tttcagcatc ttctaacacc
      121 gaattagcac gaatttcgac accatcagca attttacaat ttttaataac acaatttgtg
      181 ccaactttca ctccattacc aaaatgatta tctccttcta aaagaacgtt ggcatcaaaa
      241 aatacatcca tgcctatagt cgctgtacca cgcatatcaa accgtgcagg atcaataatc
      301 gttgcacctg cacgcattaa gttatctgct tgtaaacgtt gatacacgcg ctccagtgtt
      361 gataattgta atttatcatt aatcccttca acttcccatg ccgcttgcgg ctggtaggta
      421 tgtaccgtta aaccttgctg cacagctaaa gcaatcacat ccgttaaata atattcgcct
      481 tgggcattat tgttttttaa ggtgggtaac cactgatgca aatgacaatt aggcacacac
      541 aaaatgcctg tattgacttc agtaatttgt ttttcttgct ctgaggcgtc tttttgctca
      601 acaatacgtt gcaccaaacc tgcttcacga acaatgcgac cataacctgt tggatttgct
      661 aaattaacgg ttaacagtgc tattttttga ttatcgacca ctaaatctaa taatttttgt
      721 aaggtttcgg gggtagttaa tggcacatca ccatatagaa ttaaactttt acctgtttca
      781 ggtaattgtg ataaagccat ttgtaccgca tggcctgtgc ctttttgttc tgcttgtaac
      841 gcccatgcta aattttcttg atgagcaaaa tgcttttgta caacttcgcc gccatgacca
      901 taaacaacaa tggtgcggct agattgtaac tcttgtgcag ttgccaacac atgcgccaat
      961 aagggttttc ctgccaaggg ctgcatcact tttggcaata ctgacttcat gcgagtacct
     1021 ttacccgccg ctaaaataat gacacttaat tccatcttaa actacctaaa acaaaaaaag
     1081 gcagcataaa tgctaccttt ttatggggtt ttgcattgtg gcgaaacaat accgcaaagc
     1141 tagtcgttat gcacgatttt ttagctgttg cagagtacgc aattgacctg ctgcttcggc
     1201 taaagcggct aatgcagcac ctgtatccat ttcggatttt tggttttgca aagctgccaa
     1261 cgcatcttca cgcgctttaa ttgccgctgc ttcgtcaatg ccatcagcac gtaccacagc
     1321 gtcagccaaa acagtgacaa tatgtggttg aacttctaac acaccaccag acacataaaa
     1381 cacttcttcc tgaccgcctt ctttaatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnntat tcaccagcca aaatgccttt gaagccacgg atggtttctt ttaacgaaac
     1561 atacttacct ggagaacctg taaatacttc cgctacgtgg aatggctgag ataagaaacg
     1621 ttggatttta cgtgcacggt aaaccactaa cttatcttct tcagacaatt cgtccatacc
     1681 taagatggcg ataatgtctt tcaattcttt gtaacgttgc aatacagttt gtacaccacg
     1741 ggcaacagta tagtgctctt caccaaccac taaaggatct aattggcggc tagtagaatc
     1801 taacgggtca accgctggat aaatacctaa agaagcaata tcacggctca aaactacagt
     1861 cgcgtctaag tgagcgaagg ttgttgcagg tgatgggtca gtcaagtcgt cggctggaac
     1921 gtaaacggct tgcacagagg taatagaacc agacttagtc gaggtaatac gctcttgtaa
     1981 tacacccatt tcttcggcca atgtgggttg ataacctacc gcagatggca tacgacctaa
     2041 caatgcagat acttctgtac ctgctaaggt gtaacggtaa atgttgtcaa cgaacaacaa
     2101 tacgtcacgg cctttaccgc tttcgtcttt ttcatcacgg aagtattcag ccatggtcaa
     2161 accagtcaag gcaacacgta aacggtttcc aggtggctcg ttcatctgac cgtaaaccat
     2221 cgctacttta ccaacaacac cagaatcttt catttcatgg tagaagtcgt taccttcacg
     2281 agtacgctca cctacaccag caaacacgga taaaccagag tgtgctttag cgatgttgtt
     2341 gatgagttcc atcatgttaa cggttttacc tacaccagca ccaccgaaca aaccaacttt
     2401 accaccctta gcgaatggac agagtaagtc gataacttta ataccggttt cgagtaattc
     2461 tgttgcacca gattgctcat catagcttgg cgctttacgg tgaatagacc attgagcctg
     2521 ttcaccaatt gggccagctt cgtcaattgg attacccaac acatccataa tacgacctaa
     2581 agtcgctgta ccgactggca cagagatagg cgcattggtg ttgctaacgg gcaaaccacg
     2641 cttcaaacct tcagttgaac ccatggcgat ggtacgtaca ataccgtcac cgagttgttg
     2701 ttgtacttct aaagtcgtgg cagtgccatc aacttttaaa gcgtcataga cttttggcac
     2761 gctgtcgcgc ggaaattcaa catcaataac cgcaccgata atctgaacga tttgaccgct
     2821 gctcatcggg atttcctctt aaaactggca aatactgaat tctttacaat cttaattaag
     2881 attgagatta aaccgcggct gcaccaccga cgatttcaga aatttcacga gtaatcgctg
     2941 cttgacgtaa tttgttataa accaattgca aactacggat taactcacct gcgttatcgg
     3001 ttgcggcttt catcgccacc atacgcgcag cttgttcaca agcaatattc tctactacac
     3061 cttgatacac ttgagattca ataaaacgaa ccattaaggc atctaacaaa ggcgcagctt
     3121 caggttcata caagtaatcc caactatgct cgcgttttaa cggttcatcg ccgttgtctg
     3181 cgctcaatgg caccaattgg ttgactacag gtttttgggt catggtgtta acaaactggt
     3241 tgctgaccaa ataaatacgg tcaatttcac cacggtcaaa ggcatctaac atgaccttga
     3301 ctgccccaat taactggtct aaggacggag catcacctaa ttgggttttc accgcaacga
     3361 ctttaccacc gaccgattta aagaaagtag tggctttttg gccaatcaag cagaactctg
     3421 cttctactcc ctgttcacgc catgtcttga ctgactgcac agtacgttta aacaagttaa
     3481 tatttaaacc accacacaaa ccacgatcag aagacacaac aatatagccc acgcgcttaa
     3541 caggtctatc aaccatataa gcatgtttgt actctggatt agcttgtact aaatgggcaa
     3601 taacgcgacg cattttatcg gcataagggc gaccttgtgc catgcgatct tgcgctctac
     3661 gcattttaga ggccgctacc aactgcatcg ccttggtaat tttttgcgta ctcttaatgc
     3721 taacgacttt gctacgaatc tcttttaagc ctgccatagt ctcaccatcg ttgttggact
     3781 gtcgcctact aattaagcca actcagagag ttggcttatt aaaggtctta ccaagactgc
     3841 gtagctttaa aagctttaat gccattgtgt aggctagctt caatgtcttt atcatagtta
     3901 gcagtggcat tgatgttatc catcaaagct ttttgctgac tattcatata gccatgtaat
     3961 gcagcttcaa aagcaccaat tttggcaact ggcacatcaa ccaaataacc ttcgttagca
     4021 gcaaaaatcg ataaagccat gtcagcaatg cttaatggag catattgttt ttgcttcatt
     4081 aactcagtaa cacgttgacc atgctctaat tgtttgcggg ttgcttcgtc taagtcagaa
     4141 gcaaactgag caaacgcagc caattcacgg tattgagcta aagaggtacg gataccacca
     4201 gataacttct tgatgatctt cgtttgagcc gcaccaccta cgcgagatac ggaaataccg
     4261 gcgttaaccg ctggacgaat accagagtta aataagctag tttctaagaa gatctgaccg
     4321 tcagtaattg aaattacgtt agttggaacg aacgcagaaa cgtcacctgc ttgagtttca
     4381 atgattggca acgcggttaa agaacctgtt tgacctttca cagcaccgtt ggtgaatttt
     4441 tctacatatt cttcagatac acgagaagca cgctctaata aacggctgtg taaatagaat
     4501 acgtcacctg ggtacgcttc acgtcctggt ggacgacgta ataacaagga gatttgacga
     4561 taagcaacag cttgcttaga caaatcatca taaataatca aggcatcttc accacgatca
     4621 cggaagtact cacccatagt acaaccagaa tatggtgcta agaattgcat nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccttcttgta agcctaagta gtcacccaac
     4801 accacagcac caaccgaatc ttgctctaag tttagagcca taccgtagat gccgccatcg
     4861 aactcaatca tttcgccata cattacatcg gctaaaccgt gaatgcggac gataccgtca
     4921 gacactttta cgactgtgcc ttcattacgg gcttcagcag tcgcgttgag atcgctaata
     4981 cgctgtttaa tcagcgagct gatctcggaa ggattcagtt gctgcatgat gcgctgttcc
     5041 tcaaaaatta tgtattcagg gtggcactca attttgccaa ttttccgcgc acggaagcat
     5101 caattactaa gtcaccagta cggataatca caccaccaat taaagcagcg tcaacttcgg
     5161 tactaatatt aatacgagcc cctaactttt cagccaattg ttttgccaaa gcatcttctt
     5221 gttcatcaga taaggcaaaa gcactggtta ccgtcacatc agcggtattt tcagcttggg
     5281 cttttaaaac ttcgtataac tcagcaattg caggcaatgc cattaaacgt ttgttagctg
     5341 cgacaaggct cagaaagtta cgcacaccag cagataactc cccaccgcag gcagcaaaca
     5401 atgccgtttc ttgttcggct acagtcatgc tcgggcgtga caggtaacgg gcaaaatcag
     5461 catcttgaac aatcgcagct gcaagagcca actgagttga ccaaatggcc aactctaatt
     5521 tgccctcagc atttgcatat tcaaacgctg ctttcgcgta cggacgtgcc acagtggtta
     5581 attcagccat gttcactgtc tctagagagt tataactctg ctgctaattt gctaagcatt
     5641 gcagcatgag cattggcgtt tacttcagct tgcagaatct tttcagcacc tgcaactgct
     5701 aaagcagcta cttgagcacg taattgatca cgagcacggt ttgtctcttg ttcaatctgc
     5761 tcacgagctt gcgctttaat acgctcgcct tctacgacag cttgagactt ggcttcttca
     5821 accaattgac cagcacgacg attggcttgt tcaattaatt gagcggcttg atctttagca
     5881 gcttttaact cttgagcaac ttgatcttga gcagctttta aatcatcttg cgctttagtc
     5941 gcagcattta agccatctgc gattttcttt tgccgttcag caagcgctgc tgttaatggc
     6001 ggccatacaa acttcatgca gaaccacacg aagattgcaa aggaaatggc ctgccccagc
     6061 aaggttgcat taatattcat gctaacacct catttggctt tgcagtggat tgctctgtta
     6121 ttaagcaggt accaagccaa tgaatgggtt cgcgaatacg aagaacaaac caataccaac
     6181 accgatcatt ggtacagcgt caagcaaacc agcaacgata aacattttaa cttgcaattg
     6241 tggtgctaat tctggttgac gtgcaacgcc ttccaaaaat ttaccaccta acaaaccaaa
     6301 acctacagcg gtacccaatg caccaaaagc gatcaggata gcggcggcga tagcggtcaa
     6361 gccaatcacg gtttccattg cgaattacct cagttaagtc aaaaggaaaa ggaaaataat
     6421 tagtggtgtt cactggccat acttagatac acaatggtca acatcataaa gatgaaggct
     6481 tgtaatgtga taactaagat gtggaaaata gcccaaggca cagataacaa ccattgcaca
     6541 ccaaaaggta gcaaagcgat caagataaaa atcaattcgc cagcatacat gttaccgaat
     6601 aaccgcaaac ctaaagaaac aggcttagca atcaaagtaa caccttccaa aatcaagtta
     6661 attggaataa agaatgcctt taagattgga ttttttgggt taaatggatt taaggctaac
     6721 tcacccgcaa aaccacccac acctttcatc ttgatgctgt agaacaaaat caacataaac
     6781 acagaaatag acatacctaa tgtgatatta ggatcagtag atggcacaac tttcatatag
     6841 tgcaaaccta atgcctgtgc aaccacagga ataaagtcta ctggaattaa gtccatcaaa
     6901 ttcattaaga aaacccaaca aaaaatcgtc aacgccaaag gtgcgattaa tgaggattta
     6961 ccgtggtacg tgtctttaac gttactatca acgaactcga caatcatttc cacaaaattt
     7021 tgtacaccac ttggtacacc actgtttgct tttttagcaa cagagcgaaa caccaaacag
     7081 aaaattaaac ctaaagcaat tgaccaacct aaagagtcaa catggaacgc ccaaaagccc
     7141 atctcctgcg cttcttcagc agtatgcgca aaagaccaac catgttctgg atgatttcca
     7201 aaggtaaggt tagtcaggtg atgcttgata tattccgtag aacttccagc catatgacac
     7261 ctactttcgc tcgtcttgca gagcatccca caacggagac acccaagcta cgagctgtac
     7321 gataaagaaa cctgcgagta cccagcccac ttgcagcgat tttagacctg caaacgcgac
     7381 agagaagagc aacacggtaa taccaaaccg acctgctgct cccttataaa aactttttac
     7441 tatctgtttt gcagagcctg cccctgcatg gcgaaatgct tgtaatgcaa aatatgcact
     7501 acctattaag gcgatacttg cgcctgttgc cgctgatacc gatgaccaca caccccaaat
     7561 caaagcaatt agcgtgatgg ctgtggtggc taatacctga cctttgagta ggcgtattgc
     7621 gggggtttta tcaacaagtg gttgaggttt actcacgctt gctccaccca aaaatcattg
     7681 gcatagacaa aaaactgcta cttcgagcgc ggcgaattgt atagattgca cactaaatta
     7741 gcaagctttt gttactattt aatgtacatt taacagg
//
LOCUS       DAOQMB010000066         7756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3794_length_7756_cov_16.219322, whole genome shotgun sequence.
ACCESSION   DAOQMB010000066 DAOQMB010000000
VERSION     DAOQMB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3794_length_7756_cov_16.219322"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLY05_03870"
     CDS             <1..363
                     /locus_tag="PLY05_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02473.1"
                     /translation="KIFSKIGLKQGFQLHKNHYIQLLSEIYQPLFAESFVDSRVNDDV
                     TITLKQGNAYAWGIQYQYQYANKQGLFLHYNDTNIRYKKSSLATQSIYGQATGNYLYQ
                     PPIRFQQKTLAVGMTWQF"
     gene            complement(496..1287)
                     /gene="ampE"
                     /locus_tag="PLY05_03875"
     CDS             complement(496..1287)
                     /gene="ampE"
                     /locus_tag="PLY05_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HNC02474.1"
                     /translation="MKLILVLLVLISRELVTPAWRNLLTKPSYWWRDLWLNMANKQNL
                     SASLVLAALSLLPVMIYGVLFLFLQKAHFIWQFLWGAVILVSVFIDRRLPTVLSEARQ
                     LSLQKANVSEDQLTQVRLQLFDSYLKELFAPLFWIFLLGPWGVLLVSVYYSCRICQEQ
                     EQNLLLKDVAEKSLCYLNWLPSRALALSFALVGQFMPVWQYLLKTYQTSVSPLTLIEQ
                     SAHLAETETSTLQTEHTWLATLASYEALCFRVVVFWVILLVVQLI"
     gene            complement(1284..1850)
                     /gene="ampD"
                     /locus_tag="PLY05_03880"
     CDS             complement(1284..1850)
                     /gene="ampD"
                     /locus_tag="PLY05_03880"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HNC02475.1"
                     /translation="MNSNLEISDDGWLSLARRCPSPNFNQRPDTPISLVVVHNISLPP
                     CQFGGSYIEQLFTNCLNPNEHPYFAQIAHLQVSAHLLIRRTGEVVQFVPFTARAWHAG
                     QSLYQGRANCNDFSIGIELEGADEIPYEQVQYQQLAKIICGLKKSYPAIAEHITGHSN
                     IAPERKTDPGPAFDWSMLSRLLDQESQL"
     gene            complement(1834..4236)
                     /locus_tag="PLY05_03885"
     CDS             complement(1834..4236)
                     /locus_tag="PLY05_03885"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 domain-containing protein"
                     /protein_id="HNC02476.1"
                     /translation="MSAPQKLPAVLEGLRTVIEPKINATLHTMMTRLEEIVYQWCQGL
                     SDAEQQRYMDIIIAVRRERAQIEMDFSKALTMSFVQLPNIKPPVESGKKAPSLSGFDF
                     DSLSLVDSEDMEITVTVDSMVARTRLDYANLYSLLRQRFLHIAPSYNLIEKNFPLDTG
                     ALAQHFQNSIKHLVLEVSQKVLILRVFQQEFLSQLWPILEEANQYLVKAGILPELKST
                     FSNAAKKESDYDEVMARKEQEEKERAEKGDEPEKKLDTEDIFTFLKDVHKIGNSGPSA
                     NELPIPAAANGEVITGATYHGGEYVGGGGVGLLPASAVGGSVAAVPLVPQNIAETAKV
                     QALATEQLVGLLSKLQENQPKAKLVEDDSSPTVADVRSGIRNNLKSDEKKVEAVRQAD
                     EDVINLVSMLFDFILDDDDLPTAMKALLGRLQIPLLKVAIIDKSFFNAEKHKARQLLN
                     LLAKAGIGWNQKDPGGDALYAKIEEVVFRILNEFISDLSIFDDLLKEFSDFYEQQQKR
                     TESIDKRTREAEEGRARADMARAMVQQTLNRRLTGRQLPLVVVKLLQEGWKHVLYINC
                     LKEGTESEPWKQAVKVVDAVVWSVIPQPGAEWIARLKNVSPKLMNSLRKGLAAVNYDA
                     LATEALLKELADVHHELMGGHAMPTVTVLDPEAAKTEAPSQGTISAEQIANNSQANQA
                     TAVVLPSDSLQPALAETLPSDNEHLQKVNQLNVGSWVEFIQTDRRDRHKLVARIRSVD
                     KLIFANRRGIKVAEMSAMKLAVDMSQGRARIIEEAQVLDKALESVIGNLRTLGEQHKQ
                     QS"
     gene            4602..5441
                     /gene="nadC"
                     /locus_tag="PLY05_03890"
     CDS             4602..5441
                     /gene="nadC"
                     /locus_tag="PLY05_03890"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNC02477.1"
                     /translation="MICQTLLNQAIQQNIQLALSEDIGDGDITAQLIPANNYAKARVI
                     TREPMVLTGQPWVNALFKQLDPSVDIQWLAQEGDFLAANTPFLILAGNARSLLTGERP
                     ALNFIQTLSGTATKVRELVGLLEGTQTQLLDTRKTLPCLRIAQKYAVGVGGGKNHRLG
                     LFDAFLIKENHIMAAGSITAAVQQARLIAPNKPVEVEVETFAELEEALNAHADIIMLD
                     NFSNDDMRQAVIHTAGRAKLEASGNITKVNLREVAETGVDYISMGAITKDVKAIDLSM
                     RFI"
     gene            complement(5514..6572)
                     /gene="ftsY"
                     /locus_tag="PLY05_03895"
     CDS             complement(5514..6572)
                     /gene="ftsY"
                     /locus_tag="PLY05_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HNC02478.1"
                     /translation="MYFEEGNLDVTLPSLPAVDVPIIENPRSEALAQAEAPDVVAQPV
                     ADNSFFGRMKVGLTRTREQFANGMVTLLIGGKEIDDELLEEIETQMLVSDIGVDATRT
                     IIDRLTERVSRKELVYSNALYKALQQELVELLAPRVKPLHIDTAKKPYVILMVGVNGV
                     GKTTTIGKLAKRLQFEGKKVMLAAGDTFRAAAVEQLQVWGERNGIPVVAQSTGADSAS
                     VAFDALQSAKAKEMDVLIVDTAGRLHNKGHLMEELRKVKRVLQKVDGTAPHEVMLVVD
                     AGTGQNALNQVQEFDQVVGLTGLTITKLDGTAKGGVLFNIASRSHVPIRFIGVGEKID
                     DLRPFVAKEFVAALFQTE"
     assembly_gap    6593..6692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6774..>7756
                     /locus_tag="PLY05_03900"
     CDS             6774..>7756
                     /locus_tag="PLY05_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNC02479.1"
                     /translation="MRQYGLLALLSWPLLSSAAVTNEFSLDNGLKVVVREDQRSPVVV
                     TQVWYKIGSSYEPYGITGISHALEHMMFKGTPTVPAGEFSRLVSYLGGEDNAFTTDDY
                     TAYYQLYSNNRLPLALELEADRMANLVLKNEDFSSEIKVVMEERRQRTDDNPSGLALE
                     RFQTIAMPTSPTRNPTIGWMSDLENIKIEDLQQWYKKWYAPNNATLVIVGDVKTEDVK
                     NLVTQYFAKIPSQSLTIPPPPKELRNLGEREVKLELPAKVANLYMGFNVPSLTTATTP
                     KEVYALQMLLGVLDQGLSARLESRLVRGKRILTAVNSGYNPYSRGDTLLTIT"
BASE COUNT         2384 a         1646 c         1360 g         2266 t
ORIGIN      
        1 aagatattta gcaaaatcgg tttaaaacaa ggctttcaac ttcataaaaa tcactatata
       61 caattactta gcgaaatcta tcagccttta ttcgctgaat cctttgttga ttctagagtc
      121 aatgatgatg tcactattac gctaaaacaa ggcaatgctt acgcttgggg cattcaatat
      181 cagtatcaat atgcgaataa acaaggactt tttcttcact acaatgatac gaacatacgt
      241 tataaaaaaa gtagccttgc tacacaaagt atttatggcc aagcaacagg aaattattta
      301 tatcaaccac caattcgttt tcaacaaaaa acacttgccg ttggtatgac atggcaattt
      361 tagaacttac gcggttattt atcgcttatt tgctcactaa aaaatctctt ttataattac
      421 tgagctgagt cgaagtaacg gtttttagtt cataaaacgc gctaatttcg gcgaaatgcc
      481 taatccaaga cttgcttaaa tgagttgtac aaccaataaa ataacccaaa aaacgaccac
      541 gcgaaaacat aaagcttcat aagatgctag tgttgctaac catgtatgtt ctgtttgcaa
      601 agtactcgtt tctgtctctg ctaaatgtgc cgattgctct attaaagtta agggcgagac
      661 agaggtttga taggtcttga gcaaatattg ccaaactggc ataaattgtc caactaaagc
      721 aaaagataat gccaaagctc gactaggtaa ccaatttaaa taacacaagc ttttttctgc
      781 tacatctttg agcaataaat tttgctcttg ttcctgacaa atacggcaag aataataaac
      841 ggacaccaat aacacgcccc acggccctaa taaaaaaatc caaaacaacg gagcaaataa
      901 ttcttttaaa taactatcaa atagttgtaa acgaacctga gtcaattgat cttcgctgac
      961 gttggccttt tgcagactta attgccgagc ttcacttaat acggtaggca aacgacgatc
     1021 aataaaaaca gatactaaaa tcactgctcc ccacaaaaac tgccaaataa aatgcgcttt
     1081 ttgtaaaaac aaaaacaaaa caccataaat catgacaggc aataatgaca aagctgctaa
     1141 aacaagactt gctgataaat tctgtttatt tgccatattc aaccataaat cgcgccacca
     1201 atatgaaggt tttgttagca aattacgcca agctggggtc accaactcac gactaattag
     1261 tactaacaac actaaaatta acttcataat tgactctctt ggtcaagcaa acggcttaac
     1321 attgaccaat caaacgcagg accaggatca gtttttcgct caggggcgat attggaatgc
     1381 cctgtaatat gctcagcaat cgctggataa gactttttta gaccacaaat aatctttgcc
     1441 agttgttgat attgtacctg ttcatagggg atctcgtccg ctccctctaa ctcaatacca
     1501 atagaaaaat cgttacaatt tgcacgtcct tgatacaggg attgtcctgc atgccatgcg
     1561 cgtgctgtaa aaggtacaaa ctgcactact tcaccagtac gacgaatcaa taggtgagca
     1621 gaaacttgca aatgagcaat ttgagcaaaa taaggatgct cattaggatt taaacaatta
     1681 gtaaaaagct gctcgatgta ggaaccacca aactgacaag gtggcaaact aatattatga
     1741 acaaccacta aagatattgg cgtatctgga cgttgattaa aatttgggga aggacaacgg
     1801 cgggctagac ttaaccaacc gtcatcactg atctcaagat tgctgtttat gttgttcgcc
     1861 aagggtacgt aagttaccaa tcaccgactc caatgcttta tctaagactt gtgcttcttc
     1921 aataattcgt gcccgacctt gactcatatc aacagctaat ttcatggcac tcatttcggc
     1981 aactttaata ccgcgcctat tggcaaaaat caacttatca acggaacgaa ttctcgcgac
     2041 taacttatga cgatcacgac gatcagtttg aataaactct acccaactac cgacattaag
     2101 ctgattcact ttttgcaaat gttcattatc gctaggtaag gtttctgcta atgcaggttg
     2161 taaactatca cttggtagca caacagccgt tgcttgattt gcttgactat tattagcaat
     2221 ttgctctgca gaaatagtac cttggctagg agcttcagtc ttagccgctt ctgggtctaa
     2281 aactgtgact gtgggcatgg catgaccacc catcaactca tgatgaacat ccgccaactc
     2341 ttttaataat gcttctgttg ccaaggcgtc ataattaacc gctgctaagc ctttacgtaa
     2401 actattcatg agcttaggcg aaacattttt taaacgagct atccattctg cgcctggttg
     2461 tggaatgaca ctccacacca ccgcatcaac gactttaacg gcttgtttcc acggctctga
     2521 ttctgtacct tcttttagac agttgatata taagacgtgc ttccatcctt cttgcaataa
     2581 cttaacaacg actaaaggta actgacgacc agtcaaacgg cgatttaagg tttgttgcac
     2641 catcgcacga gccatatctg cacgagcacg gccttcctca gcttcacgag tacgtttatc
     2701 aatagactcc gtacgctttt gttgttgctc ataaaaatca gaaaattcct ttaataaatc
     2761 atcaaaaata cttaaatcag aaataaactc atttaagatt ctaaaaacaa cttcttcaat
     2821 tttggcgtat aaagcatcgc caccaggatc tttttggttc caaccaatac cagctttagc
     2881 taataaattc agtaattgac gagctttatg tttttctgca ttaaagaacg atttgtcaat
     2941 aatcgccact tttaacaatg gaatttgtaa acgtcccaac aaagctttca tggctgttgg
     3001 taaatcgtca tcatctaaaa tgaaatcgaa taacatggaa acaaggttga taacatcttc
     3061 gtcagcctga cgcaccgctt caactttttt ctcatcagat tttaaattat tacgaatacc
     3121 agaacgtaca tcagcaactg ttggtgagga atcatcttcg actaatttgg ctttaggttg
     3181 attttcttgt aattttgata ataagcctac taattgctct gtcgctaatg cttgtacttt
     3241 ggctgtttca gcaatatttt gcggcactaa aggtacagca gccacactac caccaactgc
     3301 tgacgcaggt aacaaaccta caccgccacc gccaacatat tcaccaccat gataagtagc
     3361 acccgtaata acttcaccat tcgcagcagc tggaattggc aactcattag cagatggccc
     3421 tgaattacca attttgtgta catcttttaa aaaggtaaaa atatcttctg tatctaactt
     3481 tttctcaggc tcatcacctt tttcagctct ttctttttct tcttgctctt tacgagccat
     3541 gacttcatcg taatcactct ctttttttgc tgcattacta aatgttgatt ttagttcagg
     3601 caaaatacct gctttaacta aatattgatt agcttcttct aaaattggcc ataactgact
     3661 caaaaattcc tgttgaaata cacgtaaaat caatactttt tgagagactt ctaacactaa
     3721 atgtttaatt gaattttgaa agtgttgagc caaagcgcct gtatctaagg ggaaattttt
     3781 ctcaattaaa ttataacttg gtgcaatatg caaaaaacgt tgacgcagca aactatataa
     3841 attggcataa tctaaacgag tacgcgccac catactatcg acagtgaccg tgatctccat
     3901 atcttcacta tccactaacg ataaagagtc aaaatcaaaa ccacttaagc taggtgcttt
     3961 tttaccagac tcaacaggtg gcttaatatt aggcagttga acaaaactca tggttagtgc
     4021 tttggaaaag tccatttcaa tttgagcacg ctcacggcgc acagcaataa taatatccat
     4081 ataacgctgc tgctcagcat cacttaaccc ttgacaccac tgatacacaa tttcctcaag
     4141 gcgagtcatc atcgtatgta atgtagcgtt aattttgggt tcaataactg tacgcaaacc
     4201 ttccaatact gcgggaagct tttgtggagc actcatcaga atcgttcctg ctttaataat
     4261 ttaattcttt gtaaacataa cttatatttt gtgattaaat tcacaaaata aaataatctt
     4321 tttttaaatg tagacactgt atttttctgt tttgtatcca tcttgtttaa acaagcgttt
     4381 attgcaagtt catttatgta cgatctattt tcaaccgaca atatggattt agatgtacac
     4441 actgttttaa cacaacaatg atcaatgcaa cagacaaaca gcttataatc cttctaattt
     4501 aaccatatct aaacaatagt taaagagaca aaacagtttt tttcatggct cttaacgcct
     4561 atactctcaa attatgttca aaacttaaag atgttagcga tatgatttgt caaactttac
     4621 tcaatcaagc cattcaacaa aacattcaac tggctttaag cgaagatatt ggtgatggag
     4681 acatcaccgc acaattaatt cctgccaata actacgcaaa agcacgcgtt attacacgcg
     4741 aaccaatggt gttaactggt caaccttggg ttaatgcttt atttaagcaa ttagatccct
     4801 ccgttgacat tcagtggtta gctcaagaag gtgatttttt ggctgctaat acaccttttt
     4861 tgatattagc aggcaatgct cgtagtttat taactggcga acgccctgca ctcaacttta
     4921 ttcaaacctt atcgggaact gccacaaaag tccgtgaact cgttggcctt ttagaaggaa
     4981 cacagacaca attattagat acgcgcaaaa ctttaccctg tttgcgtatt gcacaaaaat
     5041 atgctgttgg tgttggtgga ggcaaaaacc accgcttagg tttatttgat gcgtttttaa
     5101 ttaaagaaaa tcatattatg gctgctggca gcattacagc tgccgtacaa caagctcgtt
     5161 tgattgctcc caacaaacct gttgaggtag aagtagagac ttttgccgaa ttggaggaag
     5221 ccttaaacgc gcatgcagat attattatgc ttgataactt tagcaatgat gatatgcgtc
     5281 aagcggttat tcataccgct ggtcgcgcaa aactagaagc ttcggggaat attactaagg
     5341 taaatttacg agaagtagca gaaactggtg tagattatat ttcgatgggg gcaatcacca
     5401 aagacgtgaa agccattgat ttgtcgatgc gctttattta gcaaagaagg cgttctgtat
     5461 gaatcagaac gccttaattt aaaattgacg agaaatgcat cagtcctcaa accctactcc
     5521 gtttggaata aagctgcgac aaactctttg gcaacaaacg gtcttaagtc atcaatcttt
     5581 tcacccacac caataaaacg aatgggtaca tggctacggc tcgcaatatt aaacaacaca
     5641 ccacctttgg ccgtgccatc taatttggta atggttaaac ctgttaaacc gacaacttgg
     5701 tcaaactctt gcacttgatt taacgcattt tgacctgtgc ctgcatcaac aaccaacatc
     5761 acttcatggg gtgctgtgcc atcaactttt tgcaacactc gtttaacttt gcgtaactct
     5821 tccattaaat ggcctttatt gtgtaaacgg ccagccgtat cgacaattaa cacgtccatt
     5881 tctttggctt tggcactttg caaagcatca aaggcaactg aggcagaatc tgcacctgtg
     5941 ctttgagcaa ccacaggaat gccattacgc tcgccccaca cttgcaattg ttcaacagca
     6001 gcggcacgga aagtatcgcc tgccgccaac atgacttttt tgccttcaaa ctgcaagcgt
     6061 ttggctaatt taccaatggt ggtggtttta cccacaccat tcacaccgac cattaaaatc
     6121 acatacggtt ttttggcggt atcaatatgt aaaggtttaa cacgcggtgc taataactca
     6181 actaattctt gttgtaacgc tttatacagc gcattggaat agactaattc tttacgagat
     6241 actcgttcgg ttaatctatc tataatggta cgagttgcat caacaccaat gtcagaaacc
     6301 aacatttggg tttcaatttc ttctaataat tcatcatcaa tttctttgcc accaattaat
     6361 aaggtgacca taccattggc aaattgttcg cgggtacgcg ttaagccaac tttcatgcgt
     6421 ccaaaaaatg aattgtcggc aacaggctga gcaacaacgt caggtgcttc tgcttgtgct
     6481 aatgcttcag agcgtggatt ttcaataatt ggcacatcaa cagcgggtaa acttggcaag
     6541 gtaacatcta aattaccttc ttcaaaatac acaggattat ctaaccatga tgnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttaggtg catttaaaat tggcggctat
     6721 gctaccattt tagcccctgt gggtcatgtt tattttgtgg gttatcaata attatgcgtc
     6781 aatacggatt attagctctg ttatcttggc cgttgttaag ctcagcagcg gttaccaatg
     6841 aatttagttt agacaacggt ttaaaagtcg tggtgcgtga agatcaacgc tcgcctgtgg
     6901 tggtcactca agtgtggtac aaaattggtt cgagctatga accttatggt attacgggta
     6961 tttctcatgc tttagaacac atgatgttta aaggcacacc cactgtgcct gcgggcgaat
     7021 tttcgcgttt agtgtcctat ttaggcggtg aagataatgc gtttaccact gatgactaca
     7081 cagcttatta tcaactctac agcaataatc gcctaccttt agcacttgag ctagaagccg
     7141 atcgtatggc taatttggtg ttaaaaaatg aagactttag ctccgaaatt aaagtggtca
     7201 tggaagaacg ccgccaacgt accgatgaca atccctctgg tttggcttta gagcgttttc
     7261 aaaccattgc gatgcccact agccctaccc gtaatccaac gattggttgg atgagtgacc
     7321 tagaaaacat caaaattgaa gatctgcaac agtggtacaa aaaatggtac gcgcctaata
     7381 atgcgacctt agtgattgtc ggtgatgtca aaacggaaga cgtcaaaaac ttagtcaccc
     7441 aatattttgc caaaattccc tcacaaagtt taaccattcc accgccacct aaagaattgc
     7501 gtaatttggg tgaacgggaa gtaaaactag aattacccgc taaagtggct aatttgtata
     7561 tgggttttaa tgtgcccagt ttaaccactg ccactacacc taaagaagtc tatgctctac
     7621 aaatgttatt aggcgtcctc gaccaaggtt taagcgcacg gcttgaaagt cgcttagtgc
     7681 gtggcaaacg cattttaacg gcggtcaaca gtggttataa cccttatagc cgtggtgata
     7741 ctttattaac cattac
//
LOCUS       DAOQMB010000067         7710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3827_length_7710_cov_11.648334, whole genome shotgun sequence.
ACCESSION   DAOQMB010000067 DAOQMB010000000
VERSION     DAOQMB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3827_length_7710_cov_11.648334"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(338..>1258)
                     /locus_tag="PLY05_03905"
     CDS             complement(338..>1258)
                     /locus_tag="PLY05_03905"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC02480.1"
                     /translation="QILEKNQQANQVNVYPMGALTKGLDGKQPANMFALSQAGCIAVT
                     NARHGLANDETLLRCLEYAASLELTVFFYPEEPSLANGCAHDGFTASRLGLPAIPSVA
                     ETVALAKQLLLVEETGVKAHFSQLSCKGSVELIRIAKAKGLPITADVAMHQLHLTDAA
                     IDGFNSLAHVRPPLRSEEDRLALCAAVQSGVIDAICSHHQPLNAAAKLAPFPATEAGI
                     SALETVLPLGLKLVRDGVLTEDVLWRALTINPAKILGIEAGALQLGGGVVIVDLEKTW
                     SIAPENLMSAGLNTPFLGMMVQGQVVGLYR"
     assembly_gap    1259..1358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1359..2293)
                     /locus_tag="PLY05_03910"
     CDS             complement(<1359..2293)
                     /locus_tag="PLY05_03910"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNC02481.1"
                     /translation="MHLSSAHTPSRLQLNAQGRLRHFLTTEGLNREQLTEILDFAESF
                     LDQDGAITNQPLLAGRTVMNLFFEASTRTLTTFAVAAKRLSADVVNIDIAKSSNSKGE
                     TLRDMLWNLEAMTADAFVVRHSQSGAAHFIASQVTPKVAVINAGDGRHNHPTQGMLDM
                     LTIRRHAGDFSQLTVAIIGDVRHSRVARSEIYALQTLGAKEIRVIAPRTLQPKDFQQL
                     GVKLYERLEQGLDGADVVIALRIQNERMESPLLASPEEFYALYGLTPQKLAYAKPNAI
                     VMHPGPINRGVEIASEVADGHQSLILKQVNYGIAVR"
     gene            2551..3915
                     /locus_tag="PLY05_03915"
     CDS             2551..3915
                     /locus_tag="PLY05_03915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC02482.1"
                     /translation="MAKRLKLLDSGWLMMETPETPMHVGGLMLFQLPENAPDDYMQSF
                     FQYLLQVDDVSAPFNQKLQRVLPFNLDASWIKDGNFDIEYHVRPSALPKPGRIRELLA
                     LVSRLHAQRMDQRRPMWECYLIEGIEGNRFAIYSKIHHSMVDGVAATRLMQSRMARSA
                     DEDLPPIWSAEWTKRLPKKEKRSLPLPPSPKEAFNSFANGAAQLVDLLKTPRDSNAKA
                     LFQAPKTILNHRVTPARRFAAQSWPMERIRHVAKGYHATVNDIVLAMCGGCLRDYLKS
                     YDALPATPLVAQVPVSVRAADASDDNGNAISAVQVTLGTDIGNPVDRLRAIQESMHAA
                     KNRLSHMEKADINAFTVLSNLPLMLGQATGLAGRRSTMFNVVISNVPGPKEPLYMHGA
                     KMLANYPVSLIWHGYAVNMTVHSYVDSLDFGIIACRDTVPRVQRMLDYLEDALAALEN
                     HLPK"
     gene            complement(3982..4365)
                     /locus_tag="PLY05_03920"
     CDS             complement(3982..4365)
                     /locus_tag="PLY05_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNC02483.1"
                     /translation="MKPIKITLLINILILTACSSVQSNKNKSWLGYTQTGIASYYADK
                     HQNQKTASGEVYQHKLKTAAHRSLPFGANVKVVNIENNKSIVVKINDRGPFRKGRIID
                     LSKSAFMEISEANQGLLKVQIEVVP"
     assembly_gap    4527..4626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4627..5258)
                     /locus_tag="PLY05_03925"
     CDS             complement(<4627..5258)
                     /locus_tag="PLY05_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02484.1"
                     /translation="MSESRKAGSIILANLAMFNEAVVLFENEIQPQFLLELDKAIESW
                     TKEQEWIGQFEEHTDGQTWLLPQEWLYDDAEGEKSSQARFTLGFFNGDSNAYYLADLC
                     NVGQDEMGFIFQVKYGEFGGKTAWNKFAKSVPISIVETLQGLGFNDCGKGVFFIPIKL
                     NPNLLVSAWENEDYEELMQPVIVVLENIKQSVAIFNEVFSLAKQAGLGRI"
     gene            complement(5270..6070)
                     /locus_tag="PLY05_03930"
     CDS             complement(5270..6070)
                     /locus_tag="PLY05_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC02485.1"
                     /translation="MQQLSAEKLNEFFQQLTKPTLSKGLPMFFEKLALALEEDRTNIK
                     PVLVHQSLLTDPNKLDAFFKNLEQPLQIAKQSGWLCDPWQVAGLKRDEVRNSKVLSWL
                     LDPRGSHGFGDALLLALLKEINSCLVKNEQSTFDLKVSNNCRVKEEQCPDGNQGNRVD
                     IEIDDANFYLIIEVKIDAYEQDSQLERYANLAQKRANNRIWGIIYITPSGKQSLTAGG
                     YIDKVLAISWKQLAHILSDGLPKKEVTEPHAMVTALLANSFFRHIRQF"
     gene            complement(6091..6606)
                     /locus_tag="PLY05_03935"
     CDS             complement(6091..6606)
                     /locus_tag="PLY05_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02486.1"
                     /translation="MKISAYQTALLVALMFNRSGLKRGRLTAKTLCVISGRNHLRSHF
                     IVQLQDELDDLGLILFESGRGFGLIRYSLLDGAMVLSESSHIAEELFSIEVDRDYDFS
                     DIKEELGLIEPKDTTQLDDDFNFDDEDEPVIIKKPKKKHGKKTKKMIRDEQQNLEQVQ
                     PVSDEDDEFNF"
     gene            complement(6610..7455)
                     /locus_tag="PLY05_03940"
     CDS             complement(6610..7455)
                     /locus_tag="PLY05_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02487.1"
                     /translation="MDYYIIDNIVMNQISDVIIKNHIALAAFDSVGLDAIKLIAEVEQ
                     QEQNNEVWLFMALVSCSTIEITATIEWLKNIQKQARLVKEPMLLVIHLNPCLLTQSQQ
                     RIEFLRALSDFADSVVLVPQLAADSQAQALARLPQFLINMVFRSGMISLDFGDLRNRI
                     QHGIAVFGYGLQQPLNDGSFEDISAIAEINQQWRNNYLPPAYVKQILACIEGNIDVDL
                     DEYMAIGEQLQQPQEMFRTAQNMALALGMSVKEQTESEKKYIGALFFGLDFEAFIPVV
                     DNLEG"
BASE COUNT         2285 a         1598 c         1324 g         2303 t
ORIGIN      
        1 tgagcagaga aagcttgctc taaatcggca ttgcttacgc catagcttaa gttgccaaca
       61 tataatttat tgcccatgaa agggactcct caaagaatca taactctgga tttatatttt
      121 tgacttgcaa attttttata aaccctaaac gaacaccaca aaccagatca gaccgacaca
      181 ttgcacaaaa acacaaccga acgagtcacg aagctacagc tagtgatttt tcgagtataa
      241 cctacatatt gccaatgtac gatagttact tgaaaaagtt atggtgtttt aagctactta
      301 gagaagactg gttacaattt aatcaggctt tttgggtcta acgatataaa ccaaccacct
      361 gtccttgcac catcatgccc aaaaatgggg tattaagtcc tgctgacatt aaattttcag
      421 gtgcaataga ccatgttttt tcgagatcaa caatcacgac acctccaccg agttgtaatg
      481 cacctgcttc gatacctaaa attttggctg gattaatggt taaagcacgc cataacacat
      541 cttcggttaa tactccgtca cgcactaatt ttaaacctaa gggcaaaact gtttctaaag
      601 cagagatacc agcttcagtg gcaggaaatg gtgctaactt ggctgctgca tttaagggtt
      661 gatggtgtga acaaatcgca tcaatcacac ccgactgtac tgccgcacat aaggccaaac
      721 gatcttcttc actacgtaac ggcggacgga catgtgccaa actattaaaa ccatcaatgg
      781 cggcatcggt taaatggagt tggtgcatgg caacatcggc ggtaatgggc aaacctttgg
      841 ctttggcaat acgaattaat tcaaccgaac ctttgcatga caattggcta aagtgagctt
      901 taacgcctgt ttcttcgact aataatagct gtttggctaa agcaactgtt tcagcaacac
      961 taggaatagc aggtaaacct aaacgtgatg cagtaaaacc atcatgagca caaccattgg
     1021 ccaaactggg ttcttcagga taaaagaaca ccgttaactc taaagaggct gcgtattcta
     1081 aacaacgtaa taaagtttca tcattagcca aaccatgacg cgcattagtc accgcaatac
     1141 aaccagcttg acttaaagca aacatattgg cgggttgttt gccgtctaaa cctttggtta
     1201 atgctcccat aggataaaca ttgacctgat tagcttgctg atttttctct aaaatttgnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg cactgcaatg ccataattga
     1381 cctgttttaa aattaacgat tgatgaccat ctgctacttc agaggcgatt tctacaccac
     1441 gattaattgg gccagggtgc atgacaatcg cattcggttt ggcataggca agtttttgtg
     1501 gggttaaacc atataaagca taaaactctt caggactggc taataatggt gactccatac
     1561 gttcattttg aatacgtaag gcaatcacca catcagctcc gtctaagccc tgctctaaac
     1621 gttcatacaa cttcacgcca agttgttgaa agtctttggg ctgcaaggtg cgaggcgcaa
     1681 tcactcgaat ttcttttgcg cccaaggttt gtaaagcata aatttctgaa cgagccacgc
     1741 gtgaatgacg cacatcacca ataatcgcta ccgttaactg actaaaatca cctgcatgac
     1801 gacgaatcgt taacatatcc aacatacctt gtgtgggatg attatggcga ccatcgcctg
     1861 cattaatcac cgctactttt ggtgtcactt ggctagcaat aaaatgggct gcacccgatt
     1921 gtgaatgacg caccacaaaa gcatcagcag tcatggcttc taaattccac aacatatccc
     1981 gcagcgtttc tcccttacta ttagaggact tggcaatatc aatattgacc acatcagcac
     2041 tcaaacgttt agccgccacc gcgaaagtgg ttaaggtgcg tgtagaagcc tcaaaaaata
     2101 gattcattac cgtgcgccct gctaataaag gctgattagt aattgcacca tcttgatcca
     2161 aaaaggattc ggcaaaatct aaaatctcgg ttaattgttc acggtttaag ccttcggtgg
     2221 ttaaaaaatg gcgaaggcga ccttgtgcat taagctgtaa gcggctgggc gtgtgggctg
     2281 aggacaagtg catgataatt tacggtgtta taagaaagtt ggcgtgattt tagcagaaag
     2341 agaaccgtct gttgacgttt tgagtaggct cagttaagaa actatttgta gtaataaaaa
     2401 cagcaatacg cgcaagaatg attcacaaaa cactcatata cgttacaatg gctcaaataa
     2461 ataaaaaggt gttgtggctt gtgtgtcaac ataagccatg tgcgagacga tatttcaagg
     2521 caaaaagcct tgcttttgtt gagatggatt atggctaaac gattaaaact cctcgattct
     2581 ggctggttaa tgatggaaac tcccgaaacg cctatgcatg ttggcggttt gatgttgttt
     2641 caattacccg aaaacgcacc tgatgattat atgcagtctt tttttcaata cttattacaa
     2701 gtagatgatg tttctgcacc ttttaatcaa aaactacagc gcgtattacc ttttaactta
     2761 gatgctagtt ggattaaaga cggcaatttt gatattgaat atcacgttcg tccatcagct
     2821 ttacccaaac ctggtcgaat tcgtgagtta ttagccttag tatcacgttt acatgcccaa
     2881 cgcatggatc aacgccgccc aatgtgggaa tgttatttaa ttgaaggcat tgaaggtaat
     2941 cgttttgcaa tttatagcaa aatccatcat tcgatggtcg atggtgtagc agccactcgc
     3001 ctaatgcaat cgcgaatggc gcgttctgcc gatgaagatt tgccacccat ttggtcggct
     3061 gaatggacaa aacgtttacc gaaaaaagaa aagcgtagtt tgccgctgcc accttcaccc
     3121 aaagaagcgt ttaatagttt tgctaatggt gcggcgcaac tggtggactt attaaaaaca
     3181 ccacgcgaca gtaatgcgaa agctttattt caagcaccaa aaaccattct taatcaccgt
     3241 gttacacctg ctcgacgttt tgcggcacaa tcatggccaa tggaacggat tcgtcatgtt
     3301 gccaaaggtt atcatgccac ggtcaacgat attgtcttgg ccatgtgtgg tggctgttta
     3361 cgtgattact taaaaagtta tgatgcttta cctgccacgc ctttagtcgc acaagtacct
     3421 gtttcggtgc gtgcggctga tgccagtgat gataacggta atgcgattag tgcagtacaa
     3481 gtaacattgg gtacagatat tggcaatcct gtagacagat tacgtgccat tcaagaatca
     3541 atgcacgcgg ccaaaaatcg gctatcacac atggaaaaag ccgatattaa tgcgtttacc
     3601 gtgttgagta atttgccgtt aatgttaggc caagccacag ggttggctgg cagacgcagc
     3661 accatgttta atgtggtgat ttctaatgta cccggcccga aagagccatt gtatatgcac
     3721 ggtgcaaaaa tgttggctaa ctatcctgtg tcgttaattt ggcatggtta tgcggtcaat
     3781 atgacggtac atagttatgt ggacagttta gactttggca ttattgcttg tcgtgatact
     3841 gtgccgcgtg tacaaagaat gttggactat ttggaagatg cgttggccgc gttggaaaat
     3901 catttaccaa aataaagccc cctcgcctac aaacctcacc ctagccctct cctaattagg
     3961 agagggaact tcatcgctaa tttaaggcac tacttctatt tgcactttta acaagccttg
     4021 attagcctca ctgatttcca taaatgccga tttggataaa tcaataatcc gtcctttgcg
     4081 aaatggccct cggtcgttaa tttttaccac aatactttta ttattttcta tattcaccac
     4141 tttgacattt gcgccaaagg gcaaacttct atgcgcggca gtttttagtt tatgctgata
     4201 aacctcaccg ctcgcggttt tttggttttg atgtttatcg gcataataag aagcaatgcc
     4261 tgtttgggta taacccaacc agcttttatt tttgttactt tgcactgagc tacaagcagt
     4321 taagattaag atattgatta gaagtgttat ttttatgggt ttcatggtga ggtgctgagt
     4381 tttggttgaa acagttggta ggatgggtcg aagaataaga cccatctttt ttattttgtc
     4441 gattgagtaa tgcaacccac attacattta ttcaatacgt cccaaaccag cttgtttagc
     4501 taaagaaaat acttcattaa aaatagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnntcaa tacgtcccaa accagcttgt ttagctaaag aaaatacttc attaaaaata
     4681 gctaccgatt gcttaatgtt ttctaatacg acaatcacag gttgcatgag ttcttcgtag
     4741 tcttcatttt cccatgcact taccaataag ttaggattta attttatagg tataaaaaac
     4801 acgcctttac cacaatcatt aaaacctaaa ccctgaagag tctctacaat agaaatcggg
     4861 actgattttg caaatttatt ccatgcggtt ttccctccaa actcaccata ttttacttga
     4921 aatataaacc ccatttcatc ttgtccgaca ttgcacaaat cagccaaata ataggcatta
     4981 ctatcaccat taaaaaatcc taatgtaaac cttgcttgtg atgatttttc gccctctgca
     5041 tcatcataaa gccattcctg tggcaacaac caagtttggc catctgtatg ctcttcaaat
     5101 tgtccaatcc attcttgctc ttttgtccaa gactcaatag ctttgtctaa ctctaataaa
     5161 aattgaggtt gtatttcatt ctcaaataaa acgactgcct cattaaacat tgctaaatta
     5221 gctaaaataa tactacctgc tttacgtgac tcactcattt ttctgctcct caaaattggc
     5281 gaatgtgccg aaaaaaacta tttgctaata atgccgtgac cattgcatgt ggctctgtca
     5341 cttctttttt gggtaagcca tcagacaaaa tatgcgctaa ttgtttccac gatatagcta
     5401 acaccttatc tatgtagcca cctgctgtta atgattgctt accacttggt gttatataaa
     5461 taatccccca aatacgatta ttggcacgtt tctgagcaag attggcataa cgctctaatt
     5521 gcgaatcttg ttcataagca tctattttta cttcaataat caaataaaaa ttagcatcgt
     5581 ctatttctat atcaacacga ttaccttgat taccgtcagg acattgctct tcttttactc
     5641 gacagttatt actgaccttt aagtcaaatg tggattgctc atttttgact aaacacgaat
     5701 tgatttcttt taacaatgcc aacaataagg catcaccaaa accatgactg ccacgcggat
     5761 ccaacaacca agacaacact tttgaattac gcacttcatc tctttttaaa ccagcgactt
     5821 gccaagggtc acataaccaa ccactttgtt tagcaatttg taatggctgc tctaaatttt
     5881 tgaaaaaagc atctaattta ttagggtctg ttaaaagaga ttgatgtact aaaacaggtt
     5941 taatattcgt tctatcctcc tccaatgcta aagctaattt ttcaaaaaac attggcaagc
     6001 ctttggataa tgtgggttta gtcaattgtt gaaaaaactc atttagtttt tcggcactca
     6061 gttgttgcat tatgactctc ctaaaaaact ctaaaaatta aactcatcat cttcatcact
     6121 gactggctgc acttgttcaa gattttgttg ttcgtctcta atcatttttt tggtcttttt
     6181 cccgtgtttc tttttcggtt ttttaataat tactggttca tcttcatcat caaaattaaa
     6241 atcatcatct aactgagtcg tgtctttagg ctcaataagt cctaactctt cttttatgtc
     6301 actaaaatca taatctctgt caacttctat actaaataat tcttcggcaa tgtgacttga
     6361 ctcagataac accattgccc catctaacag ggaataacga attaaaccaa aacctcgtcc
     6421 agactcaaat aaaatcaaac ctaaatcatc taattcatct tgtaactgaa caataaaatg
     6481 actacgcaga tgattacgac cactaatcac acacaaggtt tttgctgtta aacgtcctcg
     6541 ctttagaccc gaacgattaa acattaacgc aactaataat gccgtttgat atgctgatat
     6601 tttcattttt taaccctcta aattatcaac aacaggtata aatgcttcaa aatcaagccc
     6661 aaaaaacaat gctcctatat attttttttc actttctgtc tgttctttaa cactcatccc
     6721 taaagccaaa gccatatttt gtgcggttct aaacatttct tgcggctgct gaagctgctc
     6781 acctattgcc atgtattcat ccaaatccac atcaatattc ccctctatac aagctagtat
     6841 ctgcttaaca taagcaggag gtagataatt attacgccat tgttggttta tttcagcgat
     6901 tgcgcttata tcctcaaaac tgccatcatt taatggttgt tgtaaaccat aaccaaaaac
     6961 ggcaatacca tgctgtatac gatttcttaa atcaccaaaa tccaaactta tcattcccga
     7021 cctgaacacc atattaatca gaaattgtgg caatcgtgca agagcttgcg cttgactatc
     7081 tgctgctaac tgaggaacta aaaccacact atctgcaaag tcacttagtg cgcgtaaaaa
     7141 ttcaatgcgt tgctgcgatt gagttaataa acagggatta agatgtatca ctaataacat
     7201 tggttcttta actagccttg cttgtttctg tatgtttttt agccattcga tagtagctgt
     7261 aatctctatt gtggaacatg acactaaggc cataaatagc cacacttcgt tattctgttc
     7321 ctgttgctct acctcagcaa ttaacttaat tgcatctaag cccactgaat caaaagccgc
     7381 taatgcaata tgatttttaa taattacgtc tgatatttgg ttcataacta tgttatcaat
     7441 aatataataa tccaaatata gcatcaatag attaaaaaat atacaatttt ttataaaaaa
     7501 cctctataat tttgtttttg ataaaaaaat gctaaaaatg gatttttata tacttcacaa
     7561 tttaggacaa ttgaagaatt agacttatta tttttttgtg ggtttcgttc ctcaacccac
     7621 actacgccaa ccaatctcgc ttttttaaac catatcattg cccaaccccc aaaaagtatc
     7681 tacaatgaat atacaaacca attaggagtc
//
LOCUS       DAOQMB010000068         7680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_3862_length_7680_cov_15.874492, whole genome shotgun sequence.
ACCESSION   DAOQMB010000068 DAOQMB010000000
VERSION     DAOQMB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7680
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_7680_cov_15.874492"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLY05_03945"
     CDS             complement(<1..416)
                     /locus_tag="PLY05_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HNC02488.1"
                     /translation="MKKFATIVLGCVLSTTSLADNLSLDLNDDALRLNYQHNLDKNYQ
                     TDFAWTHVKDLGDSLSAGLTLTQSLNNDITAYIGGKALFQQHDHLPDGTAMAVGGALR
                     ITPAANKNVSLMASAYFAPDVLSFGDMENYQELEIRG"
     gene            947..1501
                     /locus_tag="PLY05_03950"
     CDS             947..1501
                     /locus_tag="PLY05_03950"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HNC02489.1"
                     /translation="MGYRLQTIKKGVLLALLTMFSMSSYAYASNEPSEEQVKAAFIFN
                     FAKFVEWPSHSFEAENNLNLCIAGQDKVELALKSLERKEVQGHVLKIVAVNDEPTQAK
                     LKSCHILFIARSEKSRQPQWLNAVEQQPILTIADNLDLVKQGGMISLYLEAQRVQFVV
                     NQSSTQNNGLKLSARMLQLARVPK"
     gene            1514..>3285
                     /locus_tag="PLY05_03955"
     CDS             1514..>3285
                     /locus_tag="PLY05_03955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02490.1"
                     /translation="MKSLSQLPLHYKLRRILLLSAGLGLLVALFIFASVSVVKMHQDT
                     VQRLSTLAQVTSFNSQVALTFEDAKETSNVLSSLRSDKSVIHACVVRVSGEMFAQWYQ
                     HHKYRSLIQCRTGEDTRHQWFAKYLHLKESIYLDNELIGDLYIDVDMTNSWLTLFAYL
                     VSLAGLLALALLVAAFLGMRLGKYLTEPILQLATMAEAVSATKNYSLRVMGSGPDEIG
                     HLVDRFNEMLMQMEQREAELKQHREGLELLVAQRTQELIIAKEAAETANKAKSQFLAT
                     MSHEIRTPMNGILGMSEMLLSTALDSRQRRYVETVHGSGEALLLIINDILDFSKIEAG
                     RLVLETLDFNPRLVVEDVVSLLSDQAHRKGLVLTTDIDPNLTPVINGDANRLRQILLN
                     LVGNALKFTEQGLVNISVYNHHQQAHTLDFRIADTGIGIKPEVQPLLFQPFIQADSSH
                     ARRFGGTGLGLAIVKQLVELMGGQISVYSELGQGSVFSFSLIFNAPEDLDGFLAQQNN
                     AKLFPNLRSSWQQARILLAEDIATNQEVMKAMLHGFGCDVDIVMNGEQALEVLRNSHY
                     DLVLMDCQMPDMDGFEATRLFRSF"
     assembly_gap    3286..3385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3386..4359)
                     /gene="metK"
                     /locus_tag="PLY05_03960"
     CDS             complement(<3386..4359)
                     /gene="metK"
                     /locus_tag="PLY05_03960"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNC02491.1"
                     /translation="MSDYNIFTSESVSEGHPDKIADQISDAVLDAIIAKDKYARVACE
                     TMVKTGVAIVSGEITTSAWIDLEDLVRGVINDIGYTSSAVGYDGSTCGIINIIGKQSV
                     DIAQGVDRSRSEDQGAGDQGLMFGYASNETDVLMPAPICYAHRLVERQAEVRKGGLLP
                     WLRPDAKSQVTFRYENGKPVAVDAVVLSTQHNPEIAYADLQEAVMELIIKQVLPAEWL
                     HKGTKFHINPTGQFIIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSA
                     AYAGRYVAKNIVAAGLADRCEIQVSYAIGVAEPTSISLNTFGTGKISE"
     gene            4444..5403
                     /gene="birA"
                     /locus_tag="PLY05_03965"
     CDS             4444..5403
                     /gene="birA"
                     /locus_tag="PLY05_03965"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:NF008847.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HNC02492.1"
                     /translation="MELLALLADGQFHSGVDLAKQLNVSRTTISKRVADWQALGLDID
                     TVTGKGYRLHTPIDWLNQQQIFQYIPSPIQALISEFVIYPVVSSTNDAVAQSLQKKSQ
                     SGVVCLAEMQSAGRGRRGRTWLSPPAGTFYGSVGWVFNEGFQVVEGLSLAIGVAVIQA
                     LNTCGVEGLQLKWPNDIVWQGKKLGGILIEMNAEVGGSCQVVVGVGINLALPQTVKNQ
                     IDQQVVDLREICPALNRQQVTAALLHHIVLVLSDYATQGFKAYRQTWKSYDALLGVPV
                     KVLGLTQNIEGIGAGIDEQGAFLIKTATGIEAISGGEVSIRKV"
     gene            5429..6154
                     /locus_tag="PLY05_03970"
     CDS             5429..6154
                     /locus_tag="PLY05_03970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNC02493.1"
                     /translation="MGNSRIKWWLCQDQQVIASHACWHRQNLAAFAEELPEIFHQPVD
                     FVGVSSVLDSQSNQYLTWLCESWWQKKPIFAKTSSQHLGIECGYSQPERLGIDRWLNI
                     LAVAPRQPVCVVSCGTALTIDVVAKHQHLGGFILPNMNLQLSSLVQGTQGVRPDEIEF
                     PTLNWGKSTSEAVYHGILLSCVAAIEKAHQQIEQELQQTVDLVLTGGNAEKIAQHLST
                     SHDIIPELLLLGMQRYFGHSSLR"
     gene            6206..6889
                     /locus_tag="PLY05_03975"
     CDS             6206..6889
                     /locus_tag="PLY05_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02494.1"
                     /translation="MPLVFGLLVVLNAVFLAWQFFEQQNRGQNSVVIVDQPEGKAIQL
                     LAERPDLTQGGAEVEASGVVEEEGHSKIKLQDTAACYRIGPLLDPDMARQVRSIFEKG
                     GFDVAMTSSSTGGAKYLLYIPPVSTTEKAESIVKELQKQGIDASVITESSLANGISLG
                     TVGDFDQAESIKAQVAALGYRADSKTTLATRQEQWLIVSNVYSAGKTQIDRLLAGSPQ
                     LHKEKTACN"
     gene            6906..>7680
                     /locus_tag="PLY05_03980"
     CDS             6906..>7680
                     /locus_tag="PLY05_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC02495.1"
                     /translation="MSLDASPLVSIRNLSFKRGDRAIFENISLDVARGKVTAIMGPSG
                     CGKTTTLRFIGGQLHPDEGQVLVDGQNVPDMSRKELFMARAKMGMLFQSGALFSDLSV
                     YENVAFPLRAHTHLPENLIREIVLMKLEAVGLRGAIDLMPNELSGGMQRRAALARAIA
                     LDPDLIMYDEPFAGQDPMVKGVLVTLIRTLREALGLTTIIVSHDVPETLHIADYIYII
                     ANKGIIGQGTPKELKQSDSPFVQQFLNGSPDGPVRFHYPA"
BASE COUNT         2300 a         1337 c         1640 g         2303 t
ORIGIN      
        1 ccacgaattt ctaattcctg ataattttcc atatcaccaa acgataaaac atcaggcgca
       61 aaatatgccg aagccattaa tgacacattt ttgttagcag caggggtaat acgtaatgca
      121 ccaccaacag ccatggctgt tccatcgggt aaatggtcat gttgttgaaa taaagcttta
      181 ccaccaatat aagcagtgat gtcgttgtta agactttgtg ttaaagttag acctgcggat
      241 aaagaatcgc ctaaatcttt aacatgagtc catgcaaaat cagtttgata gtttttatct
      301 aagttatgtt gataatttaa acgcaaggca tcatcgttta aatctaaaga aagattatca
      361 gccaaactgg tagtgcttaa gacacagcct aaaacgatag tagcaaactt tttcatgtga
      421 aaactcatta agtaggacaa aaacgttatt aataaccatg ttgatggtgc taaccgtagc
      481 agcatatcag caatattagt agggctatta atgctttcgg cttatttata aaaagactcg
      541 actaatttgc ctagtgatgt tttgtaatat cttgttacag attatagatc taattaacag
      601 ggcaatgaag tcttgttagt tggggatttg tgtgttttgc tctgatggag tttttttgtt
      661 tgtatttcaa ttgttgtaac taattgaaat acaaacaaac acgcgatcag taaatgattc
      721 ttctgtcgtg ataaataaag tattcgttgt catgctaagc atagaaatgg ttaacaaata
      781 attaataaca gcggctttga gagactattc tacaattgat acaggagaga ttaatttaaa
      841 tgagtgatga gttaagccaa tagttttgta tggagattga agacgggtta accgcataaa
      901 tcctatagat ttttataagg gaataggtgt aatgaggcac aaagcaatgg ggtatcgtct
      961 tcagacgatc aaaaaaggag tattacttgc tttactaacc atgtttagta tgagctcgta
     1021 tgcctatgcc tcaaatgagc caagtgaaga acaagtaaaa gccgcattta tttttaattt
     1081 tgctaaattt gttgagtggc caagccatag ttttgaagct gaaaacaatt taaatttatg
     1141 tattgcaggg caagataaag tagaacttgc tttaaaaagt ttagagcgta aagaagtgca
     1201 aggtcatgta ttaaaaattg ttgcagttaa tgacgaacct actcaagcaa aattaaaaag
     1261 ctgtcatatt ttgtttattg ctcgtagcga aaaaagccgt cagcctcaat ggttaaatgc
     1321 agttgagcag caacccattt tgactattgc agataattta gacttagtga agcagggtgg
     1381 catgattagt ttatatttag aagcacagcg cgtacaattt gttgttaatc aaagtagtac
     1441 acaaaataac ggcttaaaat tgagtgcgcg tatgttgcaa ctagcccgtg tacccaagta
     1501 gctgagagcc ataatgaaat cgcttagcca gttaccacta cattacaaat taagacggat
     1561 tttactgttg agtgcgggtt taggcttgtt ggtggcacta tttatttttg ccagcgtttc
     1621 agtcgttaaa atgcatcaag ataccgtgca gcgtttaagt accttagcac aagtcacctc
     1681 atttaatagt caagtcgcct taacatttga agatgccaaa gaaacgagta atgtcctgag
     1741 ctctttacga agtgataaat cggttattca tgcttgtgtg gtacgagtat caggagaaat
     1801 gtttgctcaa tggtatcaac atcataaata ccgatcactg attcaatgtc gtacgggcga
     1861 agatactcgt catcaatggt ttgccaaata tttacatctt aaagaatcca tttatttaga
     1921 taatgaattg attggtgatt tgtacataga tgttgatatg acaaattcat ggctaacttt
     1981 atttgcctat ttggtttcct tagcaggttt gctggcttta gcattattgg ttgctgcatt
     2041 tttagggatg cgtttgggga aatatttaac agaacctatt ttgcagttag ccacaatggc
     2101 agaggctgta tcagctacca aaaattactc tttacgtgtt atggggagtg ggccagatga
     2161 aataggtcat ttggttgatc gttttaatga aatgctgatg caaatggaac agcgcgaagc
     2221 agagttaaaa caacatcgtg aaggtttgga actcttggtt gcgcaacgaa ctcaagagtt
     2281 aattattgcc aaagaagctg ctgaaaccgc caataaagca aaatcgcaat ttttagcgac
     2341 catgagtcat gaaatccgaa cgcccatgaa tggcattttg ggtatgtcag aaatgttatt
     2401 aagtaccgca ttagattctc gccaacgtcg ttatgtcgaa acagtgcatg gctctggcga
     2461 ggctttattg ttgattatta atgatatttt ggatttttcc aaaattgaag caggccgttt
     2521 agttttagaa actttagatt ttaatccccg attagtggtt gaggatgttg tcagtctatt
     2581 atccgatcaa gctcatcgca aaggtttggt attaacgacc gatattgatc ccaatttaac
     2641 ccctgtcatt aatggagatg ctaatcgctt gcgacaaatt ttattaaatt tagtcggcaa
     2701 tgctctcaag tttaccgagc agggtttagt caatatcagt gtttacaatc atcatcagca
     2761 agcacatacc ttagattttc gtattgctga cacgggaatt ggtattaagc cagaagtaca
     2821 gcctttatta tttcaaccct ttattcaagc agatagctca catgcccgtc gttttggggg
     2881 tacaggttta ggattagcga ttgttaagca attagtcgag ctaatgggtg gacaaatttc
     2941 agtttatagt gagttagggc aaggaagtgt tttttctttt agtttgatat ttaatgctcc
     3001 agaggattta gatggctttt tagcgcaaca aaataatgcc aagttatttc ctaacttacg
     3061 aagttcttgg caacaagcac gcattttgtt agcagaagat attgccacta atcaagaagt
     3121 gatgaaagcc atgttgcatg gttttggctg tgatgttgat attgtcatga atggtgagca
     3181 agctttagaa gtattgcgta actcgcatta cgatttagtg ctaatggatt gccaaatgcc
     3241 agatatggat ggttttgaag caacccgttt atttagaagt tttgannnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnntcttc actgattttg cctgtaccaa aggtatttaa
     3421 agaaatagac gttggttcgg caacaccaat cgcgtaagac acttgaattt cacaacgatc
     3481 agccaaacca gcagcaacaa tattttttgc aacataacga ccagcataag cagcagagcg
     3541 atctacttta gaagggtctt tgccagagaa agcaccaccc ccatgacgag ccatgccacc
     3601 ataagagtca acaataatct tacgaccagt taaaccacag tcaccgactg ggccgccaat
     3661 aataaattgg cctgttggat taatatggaa ctttgtgcct ttgtgtaacc attcggcagg
     3721 taaaacttgc ttaatgatta actccatcac cgcttcttgt aaatcagcat aagcgatttc
     3781 tggattatgt tgagttgata aaaccacggc atcaaccgca acaggcttgc cattttcata
     3841 acggaaagtc acttgagact tagcatctgg acgtaaccac ggcaacaaac cacccttacg
     3901 tacttcggct tgacgttcta ctaaacgatg ggcataacaa attggtgcag gcattaatac
     3961 atcggtttcg ttactggcgt aaccaaacat taaaccttga tcacctgcac cttggtcttc
     4021 agagcgtgaa cggtcaacac cttgtgcaat atcaacagat tgtttaccaa taatattaat
     4081 aatgccacaa gttgaaccat cataaccaac tgctgaagag gtataaccaa tatcgttaat
     4141 cacaccgcgt actaaatctt ctagatcaat ccatgcgcta gtggtaatct cacccgacac
     4201 aatggctacg cctgttttga ccatcgtttc acaagcaacg cgggcatatt tatctttagc
     4261 aataatggca tctaagacag catcagaaat ttggtcagca attttgtcgg gatggccttc
     4321 agacaccgac tcactggtaa aaatattata atcgctcatg attaaacctt tgcggaacaa
     4381 aatataaaag ttcccaaaga taaaagtttt ataaccattg agcaagtaaa taagaggtaa
     4441 tcagtggaat tattagcgtt attagccgat gggcagtttc attcgggtgt tgatttagcc
     4501 aaacaattaa atgtcagtcg taccacgatt tctaaacgag tcgccgattg gcaagcactt
     4561 ggtttagata ttgatacggt gacaggcaaa ggttatcgtt tgcatacgcc aatagattgg
     4621 ttaaaccaac agcaaatttt tcaatatatt ccttcaccta ttcaagcctt aatcagtgag
     4681 tttgtgattt accccgttgt ttcatctacc aatgatgcag tggcgcaaag tttgcaaaaa
     4741 aaatcacagt ctggtgtggt gtgtttagcg gaaatgcaat cggcagggcg tggccgccga
     4801 ggtcgaacat ggttgtctcc gccagcaggc acattttatg gttcggtagg ttgggttttt
     4861 aatgaaggct ttcaagttgt tgaaggattg agtttggcga ttggtgtagc tgtgattcaa
     4921 gctttaaaca cctgtggagt tgagggctta cagttaaaat ggccaaatga tattgtttgg
     4981 cagggtaaaa aattgggcgg tattttgatt gaaatgaatg ccgaagtggg tggcagttgt
     5041 caggtggtgg ttggtgtggg tattaattta gccttgccac aaacagtaaa aaaccaaata
     5101 gatcaacagg tggttgattt acgcgaaatc tgtccagcac ttaatcgtca acaagtaacc
     5161 gctgctttgc tgcatcatat tgttttagtg ttaagtgatt atgccactca aggctttaaa
     5221 gcttatcgtc aaacatggaa gtcttatgat gctttattgg gtgttcctgt taaagttttg
     5281 gggcttacac aaaatataga agggattggt gcaggcatag atgagcaagg agcattttta
     5341 attaaaactg ctacaggcat agaggcgatt agtggtggtg aagtgtctat aagaaaggtt
     5401 taatgtgaca atacaatgtt ttattgatat gggtaactcg cgtatcaaat ggtggttatg
     5461 ccaagatcaa caagtaattg ctagtcatgc ttgttggcat cggcaaaact tggctgcttt
     5521 tgccgaagaa ttaccagaaa tatttcacca gcctgttgat tttgtgggtg tatcctctgt
     5581 tttagatagt caaagcaacc aatatttaac atggttatgt gaatcttggt ggcaaaaaaa
     5641 accgatattt gcgaaaacta gttcacagca tttaggtatt gaatgtggct atagtcagcc
     5701 tgagcgatta ggtattgatc gctggcttaa tatattagct gttgcaccta gacagccggt
     5761 ttgtgtagtc agttgtggca cagcattgac gatagacgta gtcgctaaac atcagcattt
     5821 aggcggtttt atcttaccaa atatgaactt acaattatca tccttggttc aaggaacaca
     5881 gggagtccgt ccagatgaaa tagaatttcc cacacttaac tggggaaaaa gtacaagtga
     5941 agcggtatat catggtatat tgttgtcctg tgttgctgct atcgaaaaag cacaccagca
     6001 aatagaacaa gaattacaac aaactgtaga tttagtgttg actgggggaa atgccgaaaa
     6061 aatagctcag catttgtcaa catcgcatga tattatcccc gagttattac tattgggtat
     6121 gcaacgttat tttggccatt cgtcattaag ataaagcaaa tcgcgctcta ttttaatcat
     6181 aagccgtatt ataagaggaa tggatatgcc tttagtcttt gggctgttgg tagtcttaaa
     6241 cgccgttttt ttagcttggc aattttttga gcagcaaaat cgtggtcaaa acagtgtcgt
     6301 gattgtcgat caaccagaag gcaaagcgat acaattattg gcagaacgcc ccgatttaac
     6361 gcaaggtggc gcagaggttg aggcatctgg tgttgttgaa gaagaaggcc acagcaaaat
     6421 caaattacag gatacagctg cttgttatcg tattgggcct ttattagacc cagacatggc
     6481 gcgtcaagtg cgtagtattt ttgaaaaggg tggttttgat gttgccatga cttcgtccag
     6541 tacgggaggc gcaaaatatt tattgtatat tccacctgtt tctacgactg aaaaagcaga
     6601 gtctattgtt aaagagttac aaaaacaggg tatagacgcc tctgtgatta cagaatcatc
     6661 actggcaaat ggtatttcct taggtacagt aggcgacttt gatcaagctg aaagcattaa
     6721 agcacaagtg gctgctttag gatatcgagc cgacagcaaa actactttag caacacgaca
     6781 agagcaatgg ttgattgtta gcaatgttta tagcgcaggc aaaacacaaa ttgatcgact
     6841 tttagcaggt agcccacagt tgcacaaaga aaaaacggct tgtaattaat tttaatgagg
     6901 acgtaatgtc tttagacgca tctcctttag tctctattcg taacttaagt tttaaacgtg
     6961 gcgatagagc tatttttgaa aacatcagtt tagatgtggc gcgtggtaaa gttaccgcaa
     7021 ttatgggacc atcaggttgt ggaaaaacta cgaccttacg ttttattggt ggtcaactgc
     7081 atcctgacga agggcaagtc ttagttgacg gtcaaaatgt acccgatatg agccgtaaag
     7141 agctatttat ggcgcgtgct aaaatgggta tgttgtttca aagtggcgca ttattttctg
     7201 atttatcagt ttatgaaaat gttgcctttc cattacgtgc ccatacccat ctgcctgaaa
     7261 atttaattcg tgaaattgtc ttaatgaagc tcgaagcggt tggtttgcgt ggtgcaattg
     7321 atttaatgcc aaacgaatta tcaggtggta tgcagcgtcg tgccgcttta gcgcgagcta
     7381 ttgctttaga ccccgattta atcatgtatg atgaaccatt tgctggccaa gacccgatgg
     7441 taaaaggcgt attagttacc ttaattcgta ccttgcgtga agctctaggg ttaacaacaa
     7501 ttattgtgtc acacgatgtt cccgaaacct tacatattgc tgattatatt tatattattg
     7561 ctaacaaagg cattattggt caggggacac ccaaagaatt aaaacagtct gattcaccgt
     7621 ttgtgcaaca gtttttaaat ggtagtcctg atgggccagt gcgttttcac tatcctgcct
//
LOCUS       DAOQMB010000069         7473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4022_length_7473_cov_18.760582, whole genome shotgun sequence.
ACCESSION   DAOQMB010000069 DAOQMB010000000
VERSION     DAOQMB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7473
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_7473_cov_18.760582"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..1528)
                     /locus_tag="PLY05_03985"
     CDS             complement(35..1528)
                     /locus_tag="PLY05_03985"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005294230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC02496.1"
                     /translation="MTTLCTEIAIIGSGFGGIGIAIQLQKAGFNDFVLLEKEQDVGGC
                     WRDNSYPAAACDVPSHLYSFSFEPKFDWSRKFAPQAEIYAYIRDCAQKYGLIAKIKFN
                     NEVLGAYFDQQQQLWVIETKQQTVLAKILISGCGQLNRPAYPKLKGIENFKGEKFHSA
                     RWHHDYDLTNKRVAVIGTGASAIQFVPIVAKQAKQLTLFQRSAPYVIPKPDRAYSSIE
                     QQIYAKLPMLQTLSRAAMYSNHEIRALAFTSLQIAMKPFEWQFKRHLHKTVKDPVLRA
                     KLTPDYPLGCKRILISNHYYQALAQKNVEVETDNIAEVTQTGIKTKDGKEYPVDAIIY
                     GTGFQATDFLAPMKITGRDGIDLNEAWRDGAEAYLGMTVHGFPNMFLLYGPNTNLGHN
                     SILYMLESQFAYILDALKQMRRQQWQAVEVKAQTQSVYNQWVQEQLSNTIWAAGCHSW
                     YKTESGKNTNNWVGFTLLYRYKTQHFNAQDYITQAKSAVTRLTPVAA"
     gene            1632..2642
                     /locus_tag="PLY05_03990"
     CDS             1632..2642
                     /locus_tag="PLY05_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC02497.1"
                     /translation="MWPTQRNIASMRVLCQLAEDQGISLAECLRHTHISIAQLNDAFA
                     QVTAQQELQLIENILAAYKAPDHHLGLEAGRYYHLNTFGIWGFALVNSRTLRDAINLG
                     LRYLDLTFAFNQVHLQENEDDIILMLDDKAIPASCRHFLLDRDMSSIMTMVEELFNYR
                     PPLKHVCFRYSAPKDISIYQQIFGVTPEFNAPYHGVSFAKAFLELPIPQSNPHTAAMC
                     EAQCRELLRRQQQRSGVAAKVRDFLLIQPQAMPSMEQVASHLCMSSRTLRRHLTAEQV
                     SYRLLADEVRMTLAEELLKIPSLTLEEIAIRVGYSEVSNFLHAFKRCKQQTPSQYRKT
                     LC"
     gene            2636..3856
                     /locus_tag="PLY05_03995"
     CDS             2636..3856
                     /locus_tag="PLY05_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HNC02498.1"
                     /translation="MLKQPRQASLNFILMTIFLDVLGIGLMIPVLPALIGSLTDSDHM
                     QAYWFGALAATYGIMQFFCTPLLGALSDRYGRRPVLLISIFGLGISHIMTALSNSLPI
                     LLLSRVLSGATGATFSVANAYVADITSPDERGKAFGKIGAAFGIGFIFGPVIGGSLGA
                     INLHLPFFVAAALSLCNWLYGYFVLPESLNLEKRNPIQWQKANPFGALLHLGQLKGVG
                     ILVFVFACSALAQFILQNTWVLYTELRFNWTPQQNGIALFVVGFTSALTQGVLMSRLL
                     TYFGEMRLAIVGMASSAIINLAYGLTTQSYVLYGLILLNFLSFAANPALNTVISKASS
                     EREQGLTQGALNAINSIMIVIAPIIGASLLASVSDLAKDDWRMGITFFVTATLQLFAV
                     LIALWHVKNNTKTR"
     gene            3964..5199
                     /locus_tag="PLY05_04000"
     CDS             3964..5199
                     /locus_tag="PLY05_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017725337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HNC02499.1"
                     /translation="MLDHLIADFMKNWVLYVSIPFVSGIIGYVTKVVAIRMMFAPMDF
                     IGFKIFGIPIGWQGIVPRKAEKMATISVELMSTRLITANEVFARLDPHRIAKEIEGPM
                     TAAAEEITRAVAQQYQPGLWEMMPEFARQKIIKKMQTEAPQVVANIMSEIQGDVDKYF
                     DIKHMVISNLMKDRKMLNEIFQKVGHQEFKFFSNAGFYFGFGIGLIQMVCWLLFKQPW
                     MLPAFGGFVGFFSDWAALQMMFRPMQPKKIMGITFHGLFLKRQQEVARDYAALISKQL
                     LTPANMMEELFRGALSDRIMELIHRNVREMIDAQTGSIRPLVVYAVGSQKYIEMKNVV
                     TEKILERLPETMKHMESYAEDAMDVRNTLVDRMQRLTSEEFEGMLRPAFKEDEWSLIA
                     VGAALGFLVGELQIQFMLN"
     gene            5298..6242
                     /locus_tag="PLY05_04005"
     CDS             5298..6242
                     /locus_tag="PLY05_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02500.1"
                     /translation="MSAIKQLSIASLLTLQLAYSTPILAAEENNTDDGSKGLPMMILG
                     IVAKPLLDNMVSKFYNWFGQKTGVPMASPLIPADCGENCSQGLQANNTTANPNITNNT
                     TSNTQMAFGVAVGAWVKPPRMPASEADLTQPQIWKKSSSGQGTEYTQVKLSILLTETG
                     LVAVGAQDTGNPVQLRDTFIVGKPKDGSSQNVALFPIPSEKKIEVIRIKPTMPRESLW
                     VYVLPCQVTNDTRGADVGDGNEDWQNLQTQLPSAPAQFQISQLPACPNASDVNWRDIY
                     TSAKPLNMSQNDKRLYGTVNTIDNNQWYKLEIPYSTGL"
     gene            6350..>7473
                     /locus_tag="PLY05_04010"
     CDS             6350..>7473
                     /locus_tag="PLY05_04010"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNC02501.1"
                     /translation="MLIISSLLWSETTLAGQKALVVGIKDYNYWSRLQNSRNDAEDMA
                     QTLQNLGFETTLITRNDQGEVNNERFNQALQQFSQNLRPDDRVIFFYSGHGAAIPTDS
                     GSTEGYLIASNTPAPSRGNKVAIQQNAIALKQIAETISNKIDPDVGSILFMIDACRNE
                     VINRGADVVEESTPSQPLSKQIEVIYATSNGSTSLDKLPTDNQSRNGVFTRVAIEELN
                     RHKNNQGYSSDAFFPAVKTKALQLAKAAGKTQVPAWMNDTGRESFVFVPCPADQPCIV
                     VLDNSTTINHNSSADKQFWDEIKNSTDANDYRAYLKQFPNGSFVALAESRRDKYSQST
                     KQSSVLESLRLPDTKKDMSVEEMQKERENNPNGTSIYDSL"
BASE COUNT         2180 a         1521 c         1486 g         2286 t
ORIGIN      
        1 aaaaccacaa agtctaacga ggattaggct ttagtcaagc agcaacaggt gttaatcgtg
       61 tcactgctga tttagcctga gtgatataat cttgggcatt aaaatgttgc gttttataac
      121 gatacaacaa ggtaaagcca acccaattat tggtgttttt gcccgattca gttttatacc
      181 aactatggca acctgctgcc caaatggtat tactcagttg ctcttgtacc cattgattat
      241 aaacagattg cgtttgtgct tttacctcaa ccgcttgcca ttgttgacga cgcatctgtt
      301 tgagtgcatc caaaatataa gcaaattgac tttcgagcat atacaaaatc gaattatggc
      361 ctaaattagt atttggccca tacaataaaa acatattagg aaaaccatgc acagtcatgc
      421 ccaaatatgc ctctgcacca tcacgccatg cttcattcaa atcaatacca tcacggcctg
      481 taattttcat gggggctaaa aagtcagttg cctgaaaacc tgtaccataa ataatcgcat
      541 ctacaggata ttctttgcca tctttggttt taatccctgt ttgggttact tcggcaatat
      601 tatcagtctc tacttcaaca tttttttgtg ccaaagcttg ataataatga ttagaaatca
      661 aaatacgttt acaacccaaa ggataatctg gtgtgagctt agcgcgtaaa acagggtctt
      721 ttaccgtttt atgtaagtgc cgtttaaatt gccattcaaa tggtttcatg gctatttgta
      781 aggaggtaaa cgctaaagca cgaatttcat gattactata catcgctgct cgactgagcg
      841 tttgcaacat aggtaacttg gcataaattt gctgttctat ggagctatac gcacgatcag
      901 gcttgggaat cacatacggt gcagaacgtt gaaataaagt cagttgtttg gcttgtttag
      961 caacgattgg cacaaattga atcgcactcg cacctgtgcc aatcaccgct acccgtttat
     1021 tagttaaatc ataatcatga tgccaacgcg ccgaatgaaa cttttcgcct ttaaagtttt
     1081 caatgccttt taatttagga taggcaggac gatttaattg cccacaacca ctgattaaaa
     1141 tttttgccaa tactgtttgt tgtttggttt caatcaccca taactgttgt tgctgatcaa
     1201 aatacgcacc taaaacttca ttattaaatt taattttagc aattaagcca tacttttgcg
     1261 cacaatcacg aatataggca taaatttcgg cttgtggggc aaatttgcgt gaccaatcaa
     1321 atttaggttc aaacgaaaaa gaatataaat gcgaaggcac atcacacgcg gcggcaggat
     1381 aactattatc tcgccagcaa ccacccacat cttgctcttt ttcgagcaag acaaaatcat
     1441 tgaatcccgc tttttgcagt tggatggcaa taccaatgcc gccaaaacca gaaccaataa
     1501 tggcaatttc tgtgcataat gttgtcatct gcgtactcat atagaaaaat tatttttcgt
     1561 acaataacgt actatagtgc taagatttat gttagattcg gacaccattg ataaattgga
     1621 actaatctat agtgtggcct actcaacgaa atattgccag tatgcgtgtt ctttgccaat
     1681 tagcagaaga tcaaggcatt tctttagccg aatgtttacg tcatactcat atttctattg
     1741 ctcaactaaa cgatgccttt gctcaagtca ccgctcaaca agaattacaa ttaatagaaa
     1801 atatcttagc tgcctataaa gcccctgacc atcacttagg cttagaagct ggtcgttatt
     1861 accatttaaa cacttttggt atttggggtt ttgccttagt caatagccgt actttacgcg
     1921 atgcaattaa tctcggttta cgttatttgg atttaacttt tgcttttaac caagtgcatt
     1981 tacaagaaaa tgaagatgac attatcttaa tgctagatga caaagccatt cctgcttcat
     2041 gtcggcattt tttattagac cgtgatatgt cgtctatcat gaccatggtg gaggagttat
     2101 ttaattatcg cccaccacta aaacatgttt gttttcgtta ttctgccccc aaagatattt
     2161 caatttatca acaaattttt ggggtgacac cagagtttaa tgcaccttat catggtgtca
     2221 gctttgctaa agccttttta gaattgccta ttcctcaaag taaccctcat actgccgcaa
     2281 tgtgtgaggc acagtgccga gaactgttac gtcgccaaca acaacgttct ggtgtggctg
     2341 ccaaagtccg tgatttttta ttaatacaac cccaagccat gcccagcatg gagcaagtcg
     2401 cttcgcattt atgtatgagc tcacgtactc tacgccgtca tttaactgcc gaacaagttt
     2461 cgtatcgttt attggccgat gaagtacgta tgacgcttgc cgaagaattg ctaaaaatcc
     2521 cgagtttaac cctagaagaa atcgctattc gtgtaggtta ttcggaagta tcgaattttt
     2581 tacacgcgtt taaacgctgc aaacaacaaa cgccttctca atacaggaag actttatgtt
     2641 aaagcagcca cgccaagcca gtcttaactt tattttaatg accatttttt tagatgtatt
     2701 aggcattggc ttgatgattc ctgtgttgcc tgcattaatt ggttcactca ctgactctga
     2761 ccatatgcaa gcctattggt ttggggcatt agcggctacc tatggcatta tgcaattttt
     2821 ttgtacccct ctattaggtg cgttaagtga tcgttatggt cgtcgccctg tgttattaat
     2881 ttctattttt ggtctaggca ttagtcatat tatgactgcc ttgtcgaatt ccttaccgat
     2941 tttattgctg tctcgtgttt tgagtggggc aacaggcgca actttttcag tggctaatgc
     3001 ttatgttgca gacattacca gtcctgatga acgcggcaaa gcttttggta aaattggtgc
     3061 agcttttggt attggcttta tttttggccc agtcattggt ggcagtttag gagcaattaa
     3121 tttacactta cctttttttg ttgctgcggc tttatcgtta tgtaattggt tatatggcta
     3181 ttttgtttta cccgaatcac tcaatctcga aaagcgtaat cctattcaat ggcaaaaagc
     3241 caaccccttt ggtgcattac tacatttggg tcagctaaaa ggtgtgggga ttttagtctt
     3301 tgtttttgct tgctctgccc tagcacaatt tattttacaa aatacatggg ttttatacac
     3361 cgaattacgt tttaattgga caccacaaca aaatggtata gccttatttg tagtaggctt
     3421 tacctctgcc ttaactcaag gcgtgctaat gagtcgctta ttaacttatt ttggcgaaat
     3481 gcgtttagcc attgttggca tggcatcgtc agctattatt aatttagctt atggtttaac
     3541 cactcaaagc tatgtgcttt atggcttaat tttattaaat tttttgtcgt ttgctgccaa
     3601 tcctgcactg aacacggtaa tttctaaagc cagtagcgaa cgcgaacaag gtttaacaca
     3661 aggtgcatta aatgccatta atagcatcat gattgtgatt gctcctatta ttggtgcatc
     3721 gctattggca tcagtgagtg atttggcaaa agatgattgg cgaatgggta ttacattttt
     3781 tgtgactgcc acattgcagc tattcgccgt tttgattgca ctttggcatg taaaaaacaa
     3841 tacaaagacg cgttgagtca gtacactgtc aaacgttttt tctgtggata gtttaaactc
     3901 aatattcttg ctgtttgaag ctatttgtta actgctaatt aacccttgca ttaaggatgt
     3961 tttatgcttg accacctgat tgccgatttt atgaaaaatt gggttctata tgtttctatc
     4021 ccttttgtga gtggcattat tggttacgtc acaaaggtag tcgccataag aatgatgttt
     4081 gcgccaatgg actttattgg atttaaaatc tttggcattc caattggatg gcaagggatt
     4141 gtgccacgta aagccgaaaa aatggccact atctctgtgg agttaatgag tactcgcttg
     4201 attactgcaa atgaagtatt tgctcgttta gacccacatc gtattgccaa agaaatagaa
     4261 gggccaatga cggctgctgc cgaagaaatt acccgtgctg tggctcaaca atatcagccg
     4321 ggcttatggg aaatgatgcc tgagtttgct cgacaaaaaa tcatcaaaaa aatgcaaaca
     4381 gaagccccac aagttgtggc gaatattatg agtgaaattc aaggtgatgt agataagtac
     4441 tttgacatta aacatatggt gattagcaac ttaatgaaag atcgtaaaat gcttaacgag
     4501 atttttcaaa aagtcggtca ccaagaattt aagtttttta gtaatgcagg tttttatttt
     4561 ggttttggta ttggcttaat tcaaatggtt tgttggttgt tgtttaaaca accgtggatg
     4621 ttacctgctt ttggtggttt tgttggcttc tttagcgact gggctgcttt acaaatgatg
     4681 tttagaccca tgcaacccaa aaagattatg ggcattacct tccacggttt attcttaaaa
     4741 cgtcaacaag aggtcgcccg agattatgcg gcattaatct ctaaacaact actcactccc
     4801 gcaaatatga tggaagaatt atttagaggc gcattatctg atcgcattat ggaactgatt
     4861 catcgtaatg ttcgtgaaat gatcgacgca caaacaggct caattcgccc tctagtggtt
     4921 tatgcagtag gtagccaaaa atatattgag atgaaaaatg tggtgactga aaagatttta
     4981 gaacgcctac cagaaactat gaaacacatg gagtcttatg cagaagatgc aatggatgtg
     5041 cgtaatacct tagttgaccg tatgcagcgt ttaacctctg aagaatttga aggtatgtta
     5101 cgtccagcct ttaaagaaga tgaatggtca ttaattgctg tcggtgcagc acttggtttc
     5161 ttggtaggtg aattgcaaat tcaatttatg ttgaattaag cctccctata ttcaaaggca
     5221 gtcccaacgg ctgccctact atctctttgg ataattttct gtgtacaatc gaccaaattg
     5281 tacacaggaa gaacaccatg tccgccatca aacaacttag tattgccagt cttttaacct
     5341 tacaactcgc ttatagcacg ccaattttag ccgctgaaga aaataatact gatgatggca
     5401 gcaaaggttt acccatgatg attttaggca ttgtggctaa accgctttta gacaatatgg
     5461 tgagcaaatt ttataactgg tttggccaaa aaacgggtgt gccaatggct agccctttaa
     5521 tccctgcgga ttgtggcgaa aactgttcac aagggctaca agccaataac acaaccgcaa
     5581 accccaacat caccaacaac acaacgagca atactcaaat ggcctttggt gtagctgtgg
     5641 gggcatgggt taaaccgccg cgtatgcctg caagtgaagc cgatttaacc caaccgcaaa
     5701 tctggaaaaa atcgtcttcg ggtcaaggca cagaatatac gcaagtcaaa ttgtcaattt
     5761 tattaaccga aacagggtta gtcgctgtgg gtgcgcaaga cacaggtaat cctgtgcaat
     5821 tacgcgatac ttttattgtt ggcaaaccca aagatggcag ctcacaaaac gtggcgttat
     5881 tcccgattcc cagtgagaaa aaaatcgaag tgattagaat caaaccaacg atgccccgtg
     5941 aatcattatg ggtgtatgtg ttgccttgcc aagtgaccaa tgatacgcgt ggtgcggatg
     6001 tgggtgatgg caatgaagat tggcaaaatc tgcaaaccca attaccgtct gctcctgccc
     6061 aatttcagat tagccaatta cctgcttgcc ctaatgccag tgatgttaat tggcgtgata
     6121 tttacaccag tgcaaaaccg cttaacatga gtcaaaacga caaacgtctt tatggcacag
     6181 tcaatactat cgataacaat caatggtaca aattagaaat tccttacagc acaggattgt
     6241 aatgatggct attcgcttta ttagtgctta ttttttttcc tctcccaaag ggagttctct
     6301 atcactttgg gagaaagtta gagaaagggg ttttctgata gcagaatcct tgttgataat
     6361 ctcaagtttg ttatggtcag aaaccaccct cgctggacaa aaagcccttg ttgttggcat
     6421 caaagattat aactattggt ctcgtttgca aaattcacgt aatgatgccg aagacatggc
     6481 acaaacctta caaaacttag gttttgaaac gactttaatc acacgcaacg accaaggtga
     6541 agtcaataac gaacgtttta atcaagcttt acaacaattt agccaaaatc tacgtcctga
     6601 cgaccgtgtg atttttttct attcgggaca tggggcagcg attcctactg acagtggcag
     6661 tactgaaggt tatttaatcg ccagcaatac ccccgcacct agtcgtggca acaaagtagc
     6721 gattcaacaa aacgccattg ccctaaaaca aattgccgaa actattagta ataaaattga
     6781 ccccgatgtc ggttcgattt tatttatgat tgatgcctgt cgtaatgaag tgattaatcg
     6841 tggtgcagat gtggtcgaag aatccacgcc tagccaaccc ctgtctaaac aaattgaagt
     6901 gatttatgcc acctccaacg gcagcacctc actcgataaa ctgcccaccg ataatcaaag
     6961 ccgtaatggt gtgtttaccc gcgtagcgat agaagaactc aatcgccata aaaacaatca
     7021 aggttactct tctgatgcgt tttttcccgc tgttaaaacc aaagccttgc aactggccaa
     7081 agccgcaggc aaaacccaag tgcctgcttg gatgaatgat acaggccgtg aatcgtttgt
     7141 gtttgtgcct tgtccagcag atcagccgtg tattgttgtg ctggacaaca gtaccaccat
     7201 caatcacaac agcagtgcag acaaacaatt ttgggatgaa attaaaaact ctaccgatgc
     7261 caatgattac cgcgcttatt taaaacaatt tcccaatggt agttttgttg ccttggcaga
     7321 aagccgtcgc gataaataca gccaatcgac taaacaaagt agcgtattag agtctttgcg
     7381 tttacccgac actaaaaaag acatgagtgt ggaggaaatg caaaaagagc gcgaaaacaa
     7441 tcctaatggc acgagtattt atgactcttt gca
//
LOCUS       DAOQMB010000070         7330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4147_length_7330_cov_6.614158, whole genome shotgun sequence.
ACCESSION   DAOQMB010000070 DAOQMB010000000
VERSION     DAOQMB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7330
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4147_length_7330_cov_6.614158"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="PLY05_04015"
     CDS             <1..951
                     /locus_tag="PLY05_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519785.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HNC02502.1"
                     /translation="KAQKGYALGMNAGLGNLGVSAMQFIVPVVMTMALFGAIGGESQT
                     LVKDGVSTPIWLQNAGFVWVPFIALSAIAAWFGMNDIADAKASFSEQAVIFQRKHNWI
                     MCWLYLGTFGSFIGFAAGFPLLIKGQFPDVDPAKFAFLGPLVGAITRSVGGSIADKLG
                     GAKVTFWVFLLMVLSVFAVINFMPTKGADGVLMGGNFIGFFVAFLCLFALTGVGNAST
                     FKMIPVIFLTHHERLAQVNKNISREKVMQDSNKEAAAVLGFTSAIGAYGGFFIPKSYG
                     TAIAMTGSPIAALYVFIVFYVLCLAVTWWFYSRKNAPMPC"
     gene            970..4722
                     /locus_tag="PLY05_04020"
     CDS             970..4722
                     /locus_tag="PLY05_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNC02503.1"
                     /translation="MSHLLDRLLFFKQPKESFSDGHGVKTFENRQWENAYRNRWSHDK
                     VVRSTHGVNCTGSCSWKIYVKSGLVTWETQQTDYPRTRADLPNHEPRGCARGASYSWY
                     LYSANRLKYPMIRGHLLKSWREVRQREQDPVKAWTLLQQNTAAVKNYQSHRGLGGFVR
                     SSWDEVNEIIAAANVYTAKTHGPDRIIGFSPIPAMSMVSYAAGARYLSLIGGTCMSFY
                     DWYCDLPPSSPQTWGEQTDVPESADWYNSAYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYSEVAKLSDMWVHGKQGTDAALGMALGHVVLTEHYVRHKTPYFQDYARRY
                     TDMPMLVRLVKKDGLWVAERQIRASDLPDNLGATNNPEWKTVAIDETNNQLVLPNGSI
                     GTRWGEKGKWNLEEKALSGEKTKLRLSLIGHPQQEVVSVGFPYFGNIPHEHFVHTSHG
                     DIIPRDIPVIRLTDSKGEEMLVATVYDLFLANYGVDQGISKLNVALDYDDDVPYTPAW
                     QEKVTGIPRAQVIQLAREFSETAALTEGRAMVIVGAGLNHWYHMDMIYRSIINLLVMC
                     GCVGKSGGGWSHYVGQEKLRPQTGWVPLAFGTDWVRPPRQMNGTSFFYAHTNQWRYEK
                     VQVNELLSPLANDDDWKNTSLIDCNVRAERMGWLPSAPQLNLNPLTIKALADKAGRNI
                     PDYVTSALKLGTISMSCEDPDNPVNWPRNMFIWRSNLLGSSGKGHEYFLKHLLGTQNG
                     VLGKDLGEEGRAKPAEIVWHDKAPEGKLDLLVTLDFRMSTTCLYSDIVLPTATWYEKH
                     DLNTSDMHPFIHPLSAAVDPAWESRTDWEIYKGIAKHFSKLCVGHLGVETDVVLNPIQ
                     HDTPAELAQAIDVKEWKKGQCDAVAGKTLPSIVSVERDYPNTYARFTSIGPLLAKLGN
                     GGKGINWDTKKELKLLAELNYTVAEGAAKGQPRLDTAIDAAEMILSLAPETNGHVAVK
                     AWAALSEFTGRDHSHLAVPREDDQIRYRDVQAQPRKIISSPTWSGIESEHVSYNAGYT
                     NVHELIPWRTLSGRQQLYQDHPWMRAFGEELCVYKPPVDLKTTKPALDQYSDSRKQVV
                     LNWITPHQKWGIHSTYTDNLIMLTLSRGGPIVWVSEIDAQKVGIVDNDWVEVYNVNGA
                     LTARAVVSQRIPEGAIFMYHAQEKLVNIPLSEITQKRGGIHNSVTRTVTKPTHMIGGY
                     AQLSYGFNYYGTVGANRDEFVILRKMNKVEWGNEGDLASRQAKEST"
     gene            4722..6245
                     /gene="narH"
                     /locus_tag="PLY05_04025"
     CDS             4722..6245
                     /gene="narH"
                     /locus_tag="PLY05_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNC02504.1"
                     /translation="MKVRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRQGMEYAWFNNV
                     ESKPGIGYPDDWENQERWNGGWERLSNGSIRPKMGAKWRVLANIFANPDLPQIDDYYE
                     PFTFDYTHLQQAPDSKVTPTARPVSVITGKVMDKIEKGPNWEEILGGEFSKRSADYNF
                     QNVQKEMYGEFENTFMMYLPRLCEHCLNPACVSACPSGAIYKREEDGIVLIDQDKCRG
                     WRMCVSACPYKKIYFNWESGKSEKCTFCYPRIEQGEPTVCSETCVGRIRYLGVLLYDA
                     DKIEQAASVENQGDLYQAQLDIFLNPNDPEVIAAAREEGIPDSWMEAARRSPVYKMAI
                     DWKIAFPLHPEYRTLPMVWYVPPLSPIQNAHAKGQMGSNGVIPDVSSLRIPLTYLANL
                     LTGGNEAPVKLALERMLGMRVYKREQQLHAHNNPVVLRQTGLTEAMVEEMYRYLAIAN
                     YEDRYVIPSSHREYAGRAFDIRSNCGFSFGNGCSNGDNNVDLFGKQRKANLWGGTHE"
     gene            6238..6894
                     /gene="narJ"
                     /locus_tag="PLY05_04030"
     CDS             6238..6894
                     /gene="narJ"
                     /locus_tag="PLY05_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNC02505.1"
                     /translation="MNNPNLVYRALSHLLMYPDDAMRKALPEVRDILREERRLSEPTR
                     RALLGFAEDLQAGDGWDVQETYVTLFDRTRSLSLHIFEHVHGESRDRGQAMVDLIELY
                     QKQGLVLEGSQELPDYVPLFLEFLSVYDTAEQALELLKEPLHVLQALGARLRQHDSAY
                     GLVFSAIEELIGVRPLIATQTEEEVDIDQAWQEAEVRFLDAGAPSSTSCRPQTVARGN
                     "
     gene            6896..>7330
                     /gene="narI"
                     /locus_tag="PLY05_04035"
     CDS             6896..>7330
                     /gene="narI"
                     /locus_tag="PLY05_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNC02506.1"
                     /translation="MENVAQWFNTLLFGIYPYIAVGVFLVASVIRFDREQYSWKASSS
                     QLLDKSSLRLGSNLFHIGVLFIIMGHLVGLLTPTSIYTHFITVPQKQLLAMVAGGIFG
                     VLCFTGLLLLITRRLNNPRLRATTRFSDWLVLWMLFIQVSLGL"
BASE COUNT         1915 a         1508 c         1760 g         2147 t
ORIGIN      
        1 aaagcccaaa aaggttatgc ccttggtatg aatgcaggtt taggcaattt aggtgtgtcc
       61 gcaatgcagt ttattgtgcc tgtggtgatg accatggcct tatttggagc aataggtggt
      121 gagtcacaaa ctttagtcaa agatggcgtt agtacgccta tttggttgca aaatgctggc
      181 tttgtgtggg tgccttttat tgcattatcg gccattgccg cttggtttgg tatgaatgac
      241 attgccgatg ccaaagcgtc tttttctgag caagctgtga tttttcaacg aaagcacaac
      301 tggattatgt gttggttgta tttaggtact tttggctctt ttatcggttt tgccgcaggg
      361 tttcctttgt taattaaagg ccagtttcct gatgttgacc ctgcaaagtt tgctttttta
      421 ggtcctttag ttggtgcaat tacacgttct gtaggtggtt ctattgccga taaattaggt
      481 ggtgcaaaag tcaccttttg ggtgtttttg ctcatggtgc tttcggtatt tgcggtcatt
      541 aattttatgc ccaccaaagg tgctgacggc gtattgatgg ggggcaattt tataggcttt
      601 tttgtggcct ttttatgttt gtttgcccta acaggcgtag gcaacgcctc tacatttaaa
      661 atgatacctg taattttttt aacacaccat gagcgtttag cccaagtaaa taaaaacatc
      721 agccgtgaaa aagtcatgca agactctaat aaagaagctg ctgctgtgtt gggttttacc
      781 tctgctattg gtgcgtatgg tggctttttt attcctaaaa gctacggtac agccattgct
      841 atgacaggct ctcctattgc cgcactttac gtttttattg tgttttatgt gctgtgtttg
      901 gcggtgactt ggtggtttta tagccgcaaa aatgccccaa tgccttgcta agtttttgga
      961 gaaatacaaa tgagtcattt attagataga ttgttgtttt ttaagcaacc taaagaatcc
     1021 tttagcgatg ggcatggtgt taaaaccttc gagaatcgcc aatgggaaaa tgcttatcgt
     1081 aatcgttggt cacacgacaa agtggtgcgc tctacgcatg gcgtaaactg cacaggttcg
     1141 tgtagttgga aaatttacgt taaaagcggc cttgtgactt gggagacgca acaaaccgat
     1201 taccctcgta cccgtgccga cctacctaac catgaaccgc gtggttgtgc gcgtggtgca
     1261 tcgtatagct ggtatttata cagtgcgaat cgtcttaaat atccgatgat tcgagggcat
     1321 ttgttaaagt catggcgtga agtgcgtcaa cgtgaacaag atcctgttaa agcatggaca
     1381 ttattgcagc aaaataccgc agcagtaaaa aattaccaat cgcatcgtgg tttaggtggt
     1441 tttgtgcgct cgtcatggga cgaagttaac gaaattattg ccgctgctaa tgtgtacacc
     1501 gctaaaacac atggccctga ccgtattatc ggtttttcgc caattcctgc catgtcgatg
     1561 gtgtcttatg ccgcaggtgc acgttatttg tcgctgattg gcggcacttg catgagcttt
     1621 tatgactggt actgcgattt gccgccatct agtccacaaa catggggtga gcaaactgac
     1681 gttcccgaat ctgccgactg gtataactct gcttatatca ttgcgtgggg gtctaacgta
     1741 cctcaaaccc gtacgcccga tgcccacttt tttaccgaag tccgttacaa aggcactaaa
     1801 acagttgctg taacgcccga ttattccgaa gttgctaaac tatcggatat gtgggtacac
     1861 ggtaagcagg gtactgatgc tgcattgggc atggccttgg ggcatgtagt gttaaccgag
     1921 cattatgtac gtcacaaaac accgtatttt caagattatg cgcggcgtta taccgatatg
     1981 cccatgttgg tacgtttggt caaaaaagat ggtttatggg tggctgaacg tcaaattcgt
     2041 gcctcagact tgcctgataa tttaggggca accaataacc ccgaatggaa aaccgtggcc
     2101 attgacgaaa ctaataacca attagtgttg cctaatggtt ctattggtac gcgttggggc
     2161 gaaaaaggta aatggaattt agaagaaaaa gccctatcgg gagaaaaaac taaattacgc
     2221 ttatctttaa ttggtcaccc tcaacaagaa gtggtatcgg taggctttcc ttactttggt
     2281 aatattcctc acgagcattt tgtacatacc agtcatggtg acattattcc gcgtgatatt
     2341 cctgttattc gcttaaccga tagcaaaggc gaagaaatgc ttgtggctac cgtctatgat
     2401 ttgtttttgg caaattatgg cgttgaccaa ggcattagca aactcaatgt ggccttagat
     2461 tatgatgatg atgtgccata tacccctgca tggcaagaaa aagtcacagg tattccacgc
     2521 gctcaagtga ttcaattagc gcgtgagttt tccgaaacag ccgcccttac cgaaggccgc
     2581 gcgatggtga ttgtgggggc aggtttaaat cactggtatc acatggatat gatttaccgc
     2641 agcattatta acttattggt gatgtgtggt tgtgtgggta aatcgggcgg tggttggtca
     2701 cattatgtgg gtcaagaaaa actccgtcct caaacaggtt gggtgccatt agcctttgga
     2761 acagactggg tacgcccgcc acgtcaaatg aatggtactt cgttctttta tgcgcatacc
     2821 aatcaatggc gttacgaaaa agtccaagta aatgagcttt tatcgccttt agccaatgat
     2881 gatgattgga aaaacaccag tttaattgac tgtaatgtgc gtgctgagcg tatggggtgg
     2941 ttgccttctg cgccacaact taatcttaat cctctaacta tcaaggcatt agccgataaa
     3001 gcaggacgta atattcctga ttatgtgacc agcgcactca agctgggtac catttcgatg
     3061 tcgtgcgaag accccgacaa ccccgttaat tggccgcgta atatgtttat ttggcgttct
     3121 aatttattgg gttcgtcagg caaaggccat gagtactttt taaagcattt gcttggcact
     3181 caaaatggcg tgttgggtaa agacttgggc gaagagggta gagctaaacc tgccgaaatt
     3241 gtgtggcacg acaaagcacc cgaaggtaaa ttagatttat tggtcacttt agattttaga
     3301 atgtccacca cctgtttgta ttcggatatt gtgttgccta ccgccacttg gtacgaaaag
     3361 cacgacttaa atacctccga tatgcacccc tttatccacc ctctatcggc agccgttgac
     3421 cctgcatggg aatcacgcac cgactgggaa atttataaag gcattgccaa acacttttct
     3481 aaattgtgtg taggtcattt aggggtcgaa actgacgtag tacttaatcc gattcagcac
     3541 gatacccctg ccgaattagc ccaagccatt gacgtaaaag aatggaaaaa aggccagtgt
     3601 gatgctgttg caggcaaaac cttgccaagt attgtgagtg tagagcgtga ctatcctaat
     3661 acctatgcgc gttttacctc gattggccct ttgttagcaa aattgggcaa tggcggcaaa
     3721 ggtattaatt gggataccaa aaaagagcta aaactcttag cagagcttaa ttacaccgtg
     3781 gctgaaggtg cagccaaagg ccaaccgcgc ttagataccg cgattgatgc cgccgaaatg
     3841 attttgtctt tagcccccga aaccaatggc catgtggcgg ttaaagcatg ggcagcgttg
     3901 tcggagttta caggccgtga ccatagccat ttagccgttc cgcgtgaaga tgaccaaatt
     3961 cgttaccgtg acgtgcaagc acaaccgcgt aaaattatct cctcgcctac atggtcgggc
     4021 attgaatccg aacacgtgag ttataacgca ggttatacca acgtccatga gctaattcca
     4081 tggcgtacct taagtggtcg tcagcagttg tatcaagacc atccatggat gcgcgctttt
     4141 ggtgaagagt tatgtgtgta taaaccgcct gtcgatttaa aaactaccaa acccgcgcta
     4201 gaccaataca gcgatagccg caaacaggta gttttaaatt ggattacacc gcatcaaaaa
     4261 tggggtattc attctactta taccgacaac cttatcatgc tgactttgtc acgcggtggg
     4321 ccgattgttt gggtaagcga aattgatgct caaaaagttg gtattgtgga taacgattgg
     4381 gtcgaagttt acaacgtcaa cggcgcgtta acagcgcgtg cggtcgtttc gcagcgtatt
     4441 cccgaaggcg cgatttttat gtatcacgcc caagaaaagc tggtcaacat tccactctcc
     4501 gaaatcaccc aaaaacgcgg tggcatccat aattcggtga ctcgtactgt gaccaaacct
     4561 acacacatga ttggtggtta tgcccaattg tcatatggtt ttaactatta cggtacggta
     4621 ggtgctaacc gcgacgaatt tgtcatttta cgcaaaatga acaaagtcga atggggcaac
     4681 gaaggtgatt tggcgagtcg tcaagcgaag gagtcaacat aatgaaagtt cgcgcacaaa
     4741 taggcatggt gttaaacctt gataaatgta ttggttgcca tacttgctcg gtgacttgta
     4801 aaaacgtctg gacttcacgc caaggcatgg agtacgcatg gtttaacaac gtcgaatcta
     4861 aacctggtat tggttaccct gatgattggg aaaatcaaga gcgttggaat ggcggttggg
     4921 agcgtttaag taatggctca attcgcccta aaatgggcgc aaaatggcgt gtcttagcta
     4981 atatttttgc taaccctgat ttgccccaaa ttgacgatta ttacgaaccg tttacttttg
     5041 actataccca tttgcagcaa gcccctgact caaaagtcac gcctaccgcg cggcctgttt
     5101 ctgtgattac aggcaaggtg atggacaaaa tcgaaaaagg gccaaactgg gaagaaattt
     5161 tgggcggcga gtttagtaag cgttcggcag actataactt tcaaaatgtt caaaaagaaa
     5221 tgtatggcga gtttgaaaac acgtttatga tgtatttgcc ccgtttgtgt gagcattgtt
     5281 taaaccccgc ttgtgtgtct gcttgcccga gtggcgcaat ttacaaacgt gaagaagacg
     5341 gcatcgtttt gattgatcaa gataaatgtc gcggttggcg gatgtgtgtg tctgcttgcc
     5401 catacaaaaa aatctacttt aactgggaat cgggtaaatc cgaaaagtgt accttttgtt
     5461 atccacgtat tgagcaaggc gaacctacag tttgctccga aacctgtgtc ggacggattc
     5521 gttatttagg cgtgttgtta tacgatgctg acaaaattga gcaagcagcc agtgtcgaaa
     5581 atcaaggcga cttgtatcaa gcacagttgg atattttttt gaaccccaat gacccagaag
     5641 tcattgcggc agcgcgtgaa gaaggcattc ctgactcatg gatggaagca gcacgccgtt
     5701 cacctgtcta taaaatggcg attgattgga aaattgcctt tccgttacac cctgaatatc
     5761 gcaccttgcc gatggtatgg tatgttccac cattatctcc gattcaaaat gcccatgcca
     5821 aaggccaaat gggcagcaat ggcgtgattc ctgacgtgtc ttcgttgcgt attccgctca
     5881 cttacttggc caacttgcta acaggcggca acgaagcacc cgttaaatta gccctagagc
     5941 gtatgttagg tatgcgtgtt tataagcgcg aacagcaact tcatgctcat aataatcctg
     6001 ttgtgttacg tcaaacaggt ttaaccgaag cgatggttga agaaatgtat cgctatttag
     6061 cgattgcaaa ttacgaagac cgttatgtga ttccaagctc acaccgcgaa tacgcaggcc
     6121 gtgcctttga tattcgttct aattgtggct ttagctttgg taacggctgt tctaacggcg
     6181 ataacaacgt cgatttattt ggcaaacaac gcaaagcgaa tttgtgggga ggtacacatg
     6241 aataatccta atttagtgta tagagcgtta agtcatttgt tgatgtatcc cgatgatgct
     6301 atgcgtaagg cactgcccga agtacgtgat attttgcgtg aagaaagacg tttatctgag
     6361 ccaacccgtc gtgctttgtt aggttttgcc gaagacctac aagcaggtga tggttgggac
     6421 gtacaagaaa cttacgtgac tttgtttgac cgtactcgct cattatcgtt gcatattttt
     6481 gaacacgttc atggtgagtc acgtgaccgt ggtcaagcga tggttgacct catcgaactc
     6541 tatcaaaaac aaggtttagt gttagagggt agtcaagagt tacctgacta tgtgccgttg
     6601 tttttagagt ttttatcggt ctatgacacc gcagaacaag ccttagaatt gcttaaagaa
     6661 ccgttacacg ttttgcaggc actgggtgcg cgcttgcgtc aacatgactc ggcttatggt
     6721 ttggtgttta gtgcgattga agaattaatc ggtgtacgcc cattgattgc cacacaaact
     6781 gaagaagaag tagatattga ccaagcatgg caagaggccg aagtgcgctt tttagatgcg
     6841 ggtgcgcctt cttccacgtc atgtcgtcct caaactgttg caagaggtaa ctaatatgga
     6901 aaacgtagca caatggttta atactttatt atttggtatt tatccttata ttgcggttgg
     6961 tgtttttttg gttgcttctg tgattcgttt tgaccgcgaa cagtattcgt ggaaggccag
     7021 ttcttcacag ctattagata aaagcagttt acgcctcggt agtaacctgt ttcatattgg
     7081 ggtgttattt atcatcatgg ggcatttggt cggcttatta accccaacca gtatttatac
     7141 acattttatt accgtacctc aaaaacaact cttagcgatg gttgcaggcg gcatttttgg
     7201 cgtattgtgt tttacaggtt tattactgct cattacccgc cgtttaaata atccacgttt
     7261 acgcgccacg acccgttttt cggattggtt agtattatgg atgttgttta ttcaagtgtc
     7321 tttaggatta
//
LOCUS       DAOQMB010000071         7328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4149_length_7328_cov_17.889729, whole genome shotgun sequence.
ACCESSION   DAOQMB010000071 DAOQMB010000000
VERSION     DAOQMB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7328
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4149_length_7328_cov_17.889729"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="PLY05_04040"
     CDS             complement(<1..934)
                     /locus_tag="PLY05_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC02507.1"
                     /translation="MAFDDIYTAGIASGWKVTDASILQKNQTLEADVVIVGTGAGGGT
                     AAEILSQAGLKVLMIEEGALKTSANFKDLDESRAYRELYQEGAGRASSDGAIAILQGR
                     AVGGTTVVNWTASFRTPDHTLKHWADVHQVKGHSPEDMKPWFEKMEQRLNIAPWAMAP
                     NPNNQVLNDACKKLGWEWHVIPRNVKGCWNSGYCGLGCPVNAKQSMLVSTIPTALSQG
                     AELVHHLRIEKILFSGNKVTGLLGLALDTSTHQRTGIEVQIKAKHYIIAGGALNNPAL
                     LLRSQTPDPYKRVGKRTCIHPVVLTLAQMPQQIDP"
     gene            complement(953..1507)
                     /locus_tag="PLY05_04045"
     CDS             complement(953..1507)
                     /locus_tag="PLY05_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02508.1"
                     /translation="MSTLLSRRAFLRTSTIGTIALTTATTSTLTACSSTPSTPSKGFN
                     FLREGDLKLFSALIPVVLHSMIQTETPNYQALQINILKNVDGACSNLAYKAQSEIYKL
                     LDLLDGRITRWLTTGVLDTWQQASPDDVNHFLQRWQSSSIGPFNAGYRVLSKLVAVSY
                     FSLPEAQAQSGYNHLPALYQAINS"
     gene            complement(1707..1967)
                     /locus_tag="PLY05_04050"
     CDS             complement(1707..1967)
                     /locus_tag="PLY05_04050"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HNC02509.1"
                     /translation="MPKQLIGEEIAKAKNILAIQPLPPEHHENLQAQLCDLELHLQSH
                     EQVDYERFTNLLRAVEAEIEVDHPVIAGVVSSIIRTLASMGV"
     gene            complement(1978..2907)
                     /locus_tag="PLY05_04055"
     CDS             complement(1978..2907)
                     /locus_tag="PLY05_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011911467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNC02510.1"
                     /translation="MHHHHDHHHCKTPEPHAHSHHSHAPKDYGRIFAFGIILNIVFVI
                     IEALYGWYAHSLALLADAGHNLSDVLGLLLAWSAFWLAQRKGNTQHTYGWRKASILAA
                     LINSILLLVAMGAMAWEAIQRLQQPQIIESITLIWVAACGVVINGFTAWLFMRDSEHD
                     INLRGAFLHMAGDALVSVGVIITGVLYYYYQWSWLDPVMSLIIAMIIIIGTWHLLQQS
                     LNLSLDGVPEHIKLTEVQQFLLQQNHVKAVHDLHIWALSSSEVALTAHLVVNEFPQDD
                     KLLHIISDELEHHFGIQHSTLQIVQQPLKNACY"
     gene            2938..3318
                     /gene="crcB"
                     /locus_tag="PLY05_04060"
     CDS             2938..3318
                     /gene="crcB"
                     /locus_tag="PLY05_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HNC02511.1"
                     /translation="MFNSILAISIGASIGAVGRWLLSLWLNKVFLFIPLGTLLANLVG
                     GYLIGVAFALFANHPSWPAEWRLLVITGFLGGLTTFSTFSIETVSLLQQEKWLWAAMQ
                     ISLHVLGSLVMTLMGLATVSYLRQ"
     gene            complement(3355..3534)
                     /locus_tag="PLY05_04065"
     CDS             complement(3355..3534)
                     /locus_tag="PLY05_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02512.1"
                     /translation="MQVVEMNTKMDTNVFNLQAFYGAALVDEQGREIPITEEMIVDAC
                     DELMKLWHFPMTKAA"
     gene            3648..4778
                     /locus_tag="PLY05_04070"
     CDS             3648..4778
                     /locus_tag="PLY05_04070"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase family protein"
                     /protein_id="HNC02513.1"
                     /translation="MKFENNSCLHHLHIGLLINPVAGLGGELAFKGSDHQQWQISVAE
                     QSRAYQRCYQSLKLLQPYKDRLNFSCANGLMGKQLLQDLDFEAKVVYQTNLPTQAEDT
                     IRAAQALMQQNIDLLLFVGGDGTARDLCTAGVSCPVLGIPAGVKMHSGVFALNPESAA
                     EIIYKLMSGSGVAVEQAEVRDLDELALQQGKVRAQFYGELSIPIDTKLIQQMKCASPE
                     TDELVQHEIAAFVSERLETDVVYLFGVGSTCAAIMQKLGFEHTLLGFDAILNGELIGQ
                     DLNSKQITDLISQYPCKVILTVTGGQGVLLGRGNQQLTPAILQQIGRENLWVVATPHK
                     LKSLEGRALVLDTGDASLNKAWCGLIKVTTGYEQEQLYYVGQ"
     gene            complement(4843..5772)
                     /gene="htpX"
                     /locus_tag="PLY05_04075"
     CDS             complement(4843..5772)
                     /gene="htpX"
                     /locus_tag="PLY05_04075"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017400898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNC02514.1"
                     /translation="MFRIVLFLLTNLAIVVVAGIILSVLGVGSTHTAGNTLNLANLLA
                     VCFVFGMVGSLISLFMSKWLAKRTTGTEIIETPRSAEERWLLDTVAELSQKAGIKMPE
                     VGIFPSFQSNAFATGWNKNAALVAVSSGLLDRMSRDEVRAVLGHEIGHVANGDMVTLS
                     LIQGVVNTFVMFFSRIIGNFVDRVIFKNEDGPGIAYFVTSIIADIVLGILASAIVMWF
                     SRRREFRADEAGARLAGKQSMINALRRLQAEHNMPDQMPAEMTALAIAEGQREGFSLA
                     KLFASHPAIETRIAALEALQIPDQVPNQGALWR"
     gene            complement(5980..7200)
                     /locus_tag="PLY05_04080"
     CDS             complement(5980..7200)
                     /locus_tag="PLY05_04080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005177173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC02515.1"
                     /translation="MQIAKSAKLQNVCYDIRGPVLKAANAMEEQGHRIIKLNIGNPAP
                     FGFEAPQEILADVITNLPNAIGYCDSKGLFAARKAVVQYYQQKGLFVEVGDIYIGNGV
                     SELIVMAMQALLNNGDEMLIPAPDYPLWTAAVTLAGGTPTHYLCDENNGWYPDLADIE
                     SKITPKTRGIVVINPNNPTGAVYPKHILEGLVALARRHNLIIFADEIYDKILYDGAEH
                     TSIATLCDDLLCVTFNGLSKSYRVAGFRSGWLMVSGNKRAAKDYIEGLDMLASMRLCA
                     NVQAQFAIQTALGGYQSINDLICKGGRLYEQREIAWKMLNDIPGVSCTKPEGALYLFP
                     KLDPTIYPIEDDQAFILEFLQAEKVLLVQGTGFNWPHTDHFRVVFLPHIDELKEAINR
                     LARFLAGYRARMGT"
BASE COUNT         2279 a         1401 c         1498 g         2150 t
ORIGIN      
        1 aaggatcaat ttgttgtggc atttgcgcca aggttaatac cacaggatga atacaggtgc
       61 gtttaccaac acgtttataa ggatcaggcg tttgtgagcg taataaaaga gctgggttat
      121 tgagtgcacc gccagcaata atataatgtt tggccttgat ttgaacttca atccctgtcc
      181 gttggtgagt gctagtgtct aaggctaagc ctaataaacc tgtgactttg ttgccactga
      241 ataaaatctt ttcaatgcgt aaatgatgta ctaattccgc accttgacta agagcggtgg
      301 gaattgtaga cactaacatc gattgtttgg cattcacagg gcaacctaaa ccacaatagc
      361 cgctattcca acaacctttt acattacgtg gaataacgtg ccattcccag cctagttttt
      421 tgcaggcatc attaagaact tggttgttag gattgggtgc cattgcccaa ggagcaatat
      481 tcaaccgttg ttccattttt tcaaaccaag gtttcatgtc ttcaggactg tgtcctttca
      541 cttgatgaac atccgcccaa tgtttgagcg tgtgatcagg tgttctaaaa ctagctgtcc
      601 agttaacgac ggttgtacca cccacagcac gtccttggag aatagcaata gcaccatcac
      661 tactcgcacg acccgcacct tcttgataca gttcacgata agcacgactt tcatctaagt
      721 ctttaaagtt agcagaagtt tttaaagcac cttcttcaat cattaacact tttaaaccag
      781 cttgactcaa aatttctgct gctgtaccgc cgcctgcacc tgtgcccaca atcacaacat
      841 ctgcttctaa agtttggttt ttttgtagga tagaagcgtc agttactttc caacccgagg
      901 caatgcctgc ggtataaata tcatcaaatg ccatgtttag tatgtcctgt ttttagctat
      961 taattgcttg gtagagggca ggaaggtggt tataaccact ttgtgcttgg gcttcgggta
     1021 aactaaaata ggagacggcg actaatttgc ttaatacacg ataacctgca ttaaatggcc
     1081 caatcgaact gctttgccag cgttgtaaaa aatgattaac atcgtctgga ctggcttgct
     1141 gccatgtatc taacacgcct gtggttaacc aacgggtaat acggccatcc aacaaatcta
     1201 agagtttgta aatttcactt tgcgctttat aagccaaatt actacaagca ccatcaacat
     1261 tttttaagat attaatttgt aaggcttgat agttaggtgt ttcagtttga atcatgctgt
     1321 gtaagaccac aggaattaat gctgaaaata gttttaaatc gccttcgcgt aaaaaattaa
     1381 atcctttact tggcgtagat ggcgtggagc tacaagcagt taaagtgctt gtcgtggcgg
     1441 tagtcaaggc aatagtgcca atagtactcg tgcgtaaaaa agcacgtctg gataataggg
     1501 tggacataat aaattcctga gttaatccac aaaataacaa gctttaagag ttgatgggtt
     1561 gtaggattat gggtaaatat acagttgtct ttttattgtt tatagaaggg aaatcgccac
     1621 gtcaagcaat cgccattaaa aacggacgta tacgcaagaa ataccgcgta ttacactcaa
     1681 aatggggcaa aagccccatg tctcgtttaa acacccatac tcgctaaagt tcggataatg
     1741 ctgctcacaa caccagcaat tacaggatgg tcaacttcga tttcagcttc aaccgctctt
     1801 aataagttag tgaaacgttc gtaatcaact tgctcatgtg attgtaaatg taactctaaa
     1861 tcacataatt gtgcctgtag attttcgtga tgttcaggtg gcaaaggttg aatggctaaa
     1921 atatttttag ctttggcaat ttcttcacca attaattgtt ttggcattat cgctctccta
     1981 ataacaagca ttttttaaag gttgttgaac aatttgtaag gtactgtgtt gaataccaaa
     2041 atgatgttct aattcgtcac taataatgtg taataattta tcatcttgtg gaaattcatt
     2101 gacaactaaa tgagcggtta gcgcaacttc agaactgctt aatgcccaaa tatgtaaatc
     2161 atgaacggcc ttaacgtgat tttgttgtaa taaaaattgc tgtacctcgg ttagtttaat
     2221 atgttcaggg acgccatcta aacttaaatt taaagattgt tgtaataaat gccatgttcc
     2281 gataattata atcatggcaa tgattaaact catcacaggg tctaaccatg accattgata
     2341 ataatagtac aaaacaccag tgatgattac gccaacagac acaagggcat cgcctgccat
     2401 atgtaaaaaa gcgccacgca aattaatatc atgctcactg tcacgcataa atagccaagc
     2461 agtaaagcca ttaatcacaa caccacaagc agctacccaa attaaagtaa tactttcaat
     2521 gatttgaggt tgttgtaagc gttgtatcgc ttcccaagcc atagccccca tcgcgactaa
     2581 taacaaaata ctattgatta atgccgctaa aattgaggct ttacgccagc cataagtgtg
     2641 ttgtgtattg cctttacgtt gggctaacca aaaagcagac caagctaata ataatcctaa
     2701 aacatcactt aaattatgac cagcatctgc taatagagct aaagagtggg cataccaacc
     2761 atacaatgct tcaataatga caaacacaat atttaaaata ataccaaacg caaaaatacg
     2821 gccataatct ttgggggcat gagaatgatg gctatgagca tgaggctcag gtgttttaca
     2881 atgatgatgg tcgtggtgat gatgcataat aggtttaaat aaaagagttt atgttctatg
     2941 tttaactcaa ttttggcaat tagtataggg gcttctatag gcgcagtagg gcgatggttg
     3001 ttgtctttat ggctaaataa ggttttttta tttattcctt tgggaacatt gcttgctaac
     3061 ttagtgggag gctatttaat tggagtggct tttgcgctgt ttgctaatca cccaagttgg
     3121 ccagcagaat ggcgtttatt ggtgattaca ggatttttgg gcggattaac aacattctcg
     3181 actttttcaa tcgaaacggt gagtttattg caacaagaaa aatggttatg ggcggcaatg
     3241 caaataagtt tgcatgtctt gggatcatta gtaatgactt tgatgggatt ggcgacagta
     3301 agctatttaa gacaataggc agataaagtt aatctgcctt agaattggaa ataattaagc
     3361 tgctttagtc attggaaagt gccagagttt cattaactca tcgcaggcat caacaatcat
     3421 ttcttcagtg attggaattt cacggccttg ttcatcaaca agtgcagcac catagaatgc
     3481 ttgtaaatta aatacgttag tatccatttt tgtattcatt tcaacaactt gcatgataaa
     3541 cctcatttgc ctagcgcaat ttgcggggta acggcttctt tataatggta gtatagaaaa
     3601 agaattgctt tgtgtatccc taaactcact ctaatctgta accaactatg aaatttgaga
     3661 ataattcttg cttgcatcat ttacatattg gcctgttgat taatcccgtc gctggtttgg
     3721 gcggtgagct tgcctttaaa ggcagtgatc atcagcaatg gcaaattagc gttgctgaac
     3781 aatcacgtgc ctaccaacgt tgctatcaaa gcttaaaatt gttacagcca tataaagaca
     3841 gattaaattt ttcatgtgca aatggcctaa tgggtaagca attattacaa gacctcgatt
     3901 ttgaggcaaa agttgtttat caaactaatc tgccaactca ggcagaagac acaatccgtg
     3961 cagcacaggc tttaatgcaa cagaatattg atttgctact gtttgttggt ggtgacggaa
     4021 cagcacgcga tttatgtacg gctggtgtca gttgtcctgt gttgggtatt cccgcagggg
     4081 taaaaatgca ttcaggggta tttgctttga atcccgaaag tgccgcagag attatttata
     4141 aattaatgtc gggtagtggt gttgcggttg agcaagcaga agtaagagat ttagatgaat
     4201 tagccttaca acaaggtaaa gtgcgcgctc aattttatgg cgaattaagt atcccgatag
     4261 atacaaaact tatacaacaa atgaagtgtg caagcccaga aacagatgaa ttggtgcaac
     4321 acgaaatcgc tgcatttgtc agtgagcgtt tagaaacgga tgtggtgtat ttgtttgggg
     4381 taggcagtac ctgtgcggca attatgcaaa aattaggttt tgaacatact ttattgggct
     4441 ttgatgccat attaaatgga gaattaattg gccaagatct taattcaaaa caaataactg
     4501 atttaatttc tcaatatcct tgtaaagtga ttttaaccgt tactggtggg caaggtgtgt
     4561 tgttggggcg gggcaatcag cagttaacac cagcaatttt acaacagatc ggtcgagaaa
     4621 atttatgggt cgtggcaaca cctcataaat taaaaagctt agaagggaga gcattagtat
     4681 tggatacggg ggatgcctct ttaaataagg catggtgtgg cttaattaaa gtgacaacag
     4741 gttatgagca agaacaattg tattatgtag gccaataaaa aagcgaccta agtcgctttt
     4801 ttagaggttt gagcgtaaac tttcgactac gctcaaggta cattaacgcc acaaagcacc
     4861 ttgatttggc acttgatctg gaatttgtaa ggcttctaaa gcagcaatac gagtttcgat
     4921 ggctgggtga gaagcaaaca atttagctaa gctaaaacct tcacgttgac cttcagcaat
     4981 cgctaaagca gtcatctcgg caggcatttg atcaggcata ttgtgttcag cttgtaaacg
     5041 acgtaaggca ttgatcatag actgttttcc agctaaacga gcacctgctt cgtcagcacg
     5101 gaactcacga cgacgagaga accacataac aattgcagaa gccaagatac ctaaaacgat
     5161 atcagcaata atgctggtaa caaaataggc gataccaggc ccatcttcat ttttgaaaat
     5221 tacacggtca acaaagttac caataatgcg agagaagaac ataacaaagg tattcacaac
     5281 gccttgaatt aagctcaatg taaccatgtc accattcgcc acgtggccaa tttcgtgacc
     5341 tagaacagcg cgtacttcat cacggctcat acggtctaat aaaccgctag acacagccac
     5401 taaagcagca tttttgttcc aacctgtggc aaaagcattg gattgaaagc ttgggaaaat
     5461 acccacttct ggcattttga tgcctgcttt ttgagataac tcagcaacag tatctaataa
     5521 ccaacgttct tcagcactac gcggtgtttc aataatttct gtacctgtgg tgcgtttagc
     5581 cagccacttc gacataaata aagaaattaa tgaacctacc ataccaaaga caaaacagac
     5641 ggctaataaa ttagctaaat tgagcgtatt acccgctgta tgtgtcgaac caacacccaa
     5701 cacgctcaaa ataatacctg cgactacaac aatcgctaag ttggtgagca ggaagaggac
     5761 gatacggaac atctttataa actcctaaaa acggattatc cgagttattt gataaggtat
     5821 ggttgttgat tatgaattac aagatggcag tattgtatct ttgtaactga aaataggtcg
     5881 ataattgagc tgttggtcaa aaaatgcagt gtattctgta ggtcttagga tttacgcatt
     5941 gcagtacaat gcgtaaacct tgttcttgtg gggaaaggat taagtaccca ttctggctct
     6001 atagcctgcc aaaaatcgtg ctagacgatt aattgcttct ttaagttcgt caatatgtgg
     6061 taaaaatacc acacggaaat gatcggtatg aggccaatta aagccagttc cttgtactaa
     6121 caaaactttt tcggcttgta aaaactctag aatgaaggct tgatcatctt caatcggata
     6181 gatggttggg tctaatttgg gaaatagata caatgcgcct tcgggtttag tacaactcac
     6241 accaggaatg tcattgagca ttttccatgc aatttcgcgc tgttcatata aacgaccacc
     6301 tttacagatt aaatcgttga tactttgata accacctaat gccgtttgaa tagcaaattg
     6361 tgcttgtaca ttggcgcata aacgcatgga ggccaacata tccaaacctt caatatagtc
     6421 tttggcagca cgtttgttac cactaaccat taaccagcct gaacgaaatc ctgcgacacg
     6481 ataagacttg gataagccat taaaggtgac acataacaaa tcatcacata aagtcgcaat
     6541 agaggtatgt tctgctccat cgtataaaat cttgtcataa atttcgtcag caaaaataat
     6601 taaattatga cggcgagcaa gggcaacaag accttctaaa atgtgttttg gataaactgc
     6661 ccctgtagga ttattggggt taatgaccac aatcccgcgt gtttttgggg tgatttttga
     6721 ctcaatatcg gctaaatcgg gataccagcc attattttca tcgcataagt aatgggtggg
     6781 tgtgccacct gctaaagtga cagcggctgt ccacaatgga tagtcagggg ctggaataag
     6841 catttcatcg ccattgttta acaaggcttg cattgccatg acaatcagtt ctgaaacacc
     6901 attaccaata taaatatcac caacttcaac aaataaacct ttttgttgat aatattgaac
     6961 aaccgcttta cgagcggcaa ataagccttt tgaatcacag tagccaatgg cattaggcaa
     7021 attagtaatt acatccgcta gaatttcttg tggggcttca aagccaaatg gtgcaggatt
     7081 accaatattt aacttgataa tacggtgtcc ttgttcttcc atggcattgg cggcttttaa
     7141 tacagggcca cgaatgtcat aacatacatt ttgtaatttt gcggattttg caatttgcat
     7201 agtaacaaac tcagttgggc tacatctaat atattcatag tcagtgataa atctggctaa
     7261 gcattcgatt ctagcgaaaa aagcgatgaa tgttcagtag gctagacaat gttttttggt
     7321 gtataaga
//
LOCUS       DAOQMB010000072         7237 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4251_length_7237_cov_16.947368, whole genome shotgun sequence.
ACCESSION   DAOQMB010000072 DAOQMB010000000
VERSION     DAOQMB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7237
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4251_length_7237_cov_16.947368"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1582)
                     /gene="murJ"
                     /locus_tag="PLY05_04085"
     CDS             complement(2..1582)
                     /gene="murJ"
                     /locus_tag="PLY05_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNC02516.1"
                     /translation="MSQVTPPSQKSATQGAKKSGLWRSTLIVSAMTMLSRVLGLVRDM
                     VLMNAFGADKMMDAFLVAFKIPNFLRRLFAEGAFAQAFVPVLSEYKSQKGDEAVKDLV
                     SKVAGSLSLILFVVTIIAIVGAPLIMWVFAPGFSGDAEKFNLAADMLQITFPYLLLIS
                     LTAFAGGILNSYGAFALSSFTPVLLNLVMIGSALFLAPQFDIPIMALAWGVLLAGVAQ
                     LAIQIPALYNIGMLPRFKVAFADEGVKKIFTLMLPAMFGVSVSQINLLLDTILASFLV
                     SGSVAWLYTAERLTELPLGLIGIAVATVILPSLSAKHAEKSEAEFKAMLDWALRVIVL
                     VGVPSSLAMLILAEPMIAALFHHGKFSANDVAMSAAALQALSGGILAFMLIKVFAPGY
                     YSRQDTKTPVKIGIIAMISNMAFNLMLVWHFKHVGLAFASTMSAFLNAGLLYHGLHKQ
                     NVFRLEKHWRVLALRYGFANIAMVAVLLVITPSLAFWLDANALHKVAYLLAICVAGVV
                     TYLVALTLTGFKWRELKH"
     gene            1806..2069
                     /gene="rpsT"
                     /locus_tag="PLY05_04090"
     CDS             1806..2069
                     /gene="rpsT"
                     /locus_tag="PLY05_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HNC02517.1"
                     /translation="MANSAQAKKRARQNEQQRKHNASLRSMVRTYLKKVVNAIAAGDK
                     AAASAAYTAAVPVVDRMADKGIIHKNKAARHKSRLNAHIKAMA"
     gene            2188..2382
                     /locus_tag="PLY05_04095"
     CDS             2188..2382
                     /locus_tag="PLY05_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC02518.1"
                     /translation="MYVCVCHAVSDKQIKQSVENGCCSYREIRDCHKVGTTCGRCVPE
                     ARQLIDETLANLAKNISHAA"
     gene            2592..3056
                     /gene="bfr"
                     /locus_tag="PLY05_04100"
     CDS             2592..3056
                     /gene="bfr"
                     /locus_tag="PLY05_04100"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNC02519.1"
                     /translation="MKGDAQVIAYLNKALTNELTAINQYFLHARMLKSWGFEKLGQHE
                     YMESIDEMKHADKLIDRILFLEGLPNLQHLNKLYIGENIEEILQGDLKLEQLAHPDLK
                     DAIAHCEKVGDYGSRELFVAILESEEEHIDWIETQLELINKLGVQNYLQSAM"
     gene            complement(3239..4888)
                     /locus_tag="PLY05_04105"
     CDS             complement(3239..4888)
                     /locus_tag="PLY05_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02520.1"
                     /translation="MPKLPIPSSIFVRIYGGLLLIISAVALCTYLLLQVINDYRAAEY
                     RESMATGFFRLIALGIARQSSPELQQAWIEEASTLLDSKIVLSKAQQIDFTSRELEQL
                     EQGKAVVRIANNFSYADIYSAVSVEKDQYLQVRMSKVSEQQAKAMAIFLLDDLDHYRG
                     QEQKRLKQLQVYFPFPLHIMPLDKASLEPDQRARIKRKEVVLALKEGGVGHNSSVRII
                     APSSQFNQVVVIGPMYLFDWMPVQLLLIISAIALLVITTTAYLIIHPLEKKIKTVESA
                     ARRIGGGDLSARAEVDGKDEVSQLAYAFNSMAEHIQRLIDSQRELTRAVSHELRTPVA
                     RIRFGMEMLADTDDIEDRFRQLAQLDGDIDELNKLIDEILTYAKLEQGIPVLDFEAVD
                     LGELLQKVAKETTALGVKQKVTVQLPPQGTIAEAERRYLHRVLQNLAGNATRYAKSEV
                     CLSAGIYEGCAYIAVEDDGHGIPEKDREKVFQPFTRLDDSRTRASGGYGLGLSIVARI
                     AFWFGGNIKVEASPRLGGAKFVMTWPMKQKNRNRLKLNEPS"
     gene            complement(4890..5609)
                     /locus_tag="PLY05_04110"
     CDS             complement(4890..5609)
                     /locus_tag="PLY05_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC02521.1"
                     /translation="MSQQEEHLPRILIVEDDERLATLTQEYLIRNGLLVAIETDGNRA
                     IRRIIAEQPDLVVLDVMLPGADGLTICREVRPHYHHPILMLTARTDDMDQVLGLEMGA
                     DDYVAKPAQPRVLLARIRALLRRTDKIVEEDVPQRLEFDDLVIDNGGRSVTLADEPVE
                     FTSAEYDLLWLLASNAGRILSREDIFERLRGIEYDGQDRSIDVRISRIRPKIGDDPDN
                     PKRIKTVRSKGYLFVKESLGG"
     gene            6101..>7237
                     /locus_tag="PLY05_04115"
     CDS             6101..>7237
                     /locus_tag="PLY05_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004703519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HNC02522.1"
                     /translation="MTISAQRSFESTEPLATAPGVLRVIKRNGTLVTYDASKIAIAIS
                     KAFLAVEGGSAADSSRVHQQVALLTDAVSNTFKRRMPSGGTVHIEEIQDQVELALMRA
                     GEQKVARSYVIYRDERARLRDEGITKSHPTLTVTLGDGTRQPLDLGRLEALVSSACED
                     LTGVNAQAIVDETLKNLYDGVKESDLCTTMVMCSRVHIEQEPNYTYVTARLLLDQLRS
                     EALSFLDIADKASQAQMSDFYPQALPVFLKRGVELELLHPDLLKFDLAKLADAIEPER
                     DFQFTYLGLQTLYDRYFIHSNEVRFELPQLFFMRVAMGLAMNEANKEARAIEFYQLLS
                     SFDYMASTPTLFNSGTLRPQLSSCYLTTVPDDLYGIYDAIRDNAM"
BASE COUNT         2303 a         1566 c         1287 g         2081 t
ORIGIN      
        1 atcaatgctt taactcacgc catttaaagc ctgttaaggt taatgccact aaataagtca
       61 ctacccctgc tacacaaatc gccaataagt acgctacttt atgcaaagca tttgcatcta
      121 accaaaaagc caagcttggc gtaatcacta acaacacggc caccatggcg atattggcaa
      181 aaccataacg caacgctaat acgcgccaat gcttttctaa tctaaagaca ttttgtttgt
      241 gtaaaccgtg atacaacaaa cctgcattta aaaaggccga catggtacta gcaaaggcca
      301 agcccacgtg tttaaagtgc caaaccagca ttaaattaaa ggccatattg ctaatcatgg
      361 cgataatgcc aattttaacg ggcgttttgg tgtcttggcg cgaataataa ccaggcgcaa
      421 ataccttaat taacataaag gctagaatcc cacctgatag agcttgtaaa gctgccgccg
      481 acattgccac atcatttgcc gaaaatttac catgatgaaa taaagcggca atcatcggct
      541 cggccaaaat caacattgct aaagaagacg gcacacctac caagacaatg actcgtaacg
      601 cccaatctaa catggcttta aattcagcct cagatttttc ggcatgtttt gccgacaatg
      661 agggtaaaat gactgttgct acggcaatgc cgattaagcc taaaggtaat tcggttaaac
      721 gctctgcggt atataaccaa gccaccgaac ctgacactaa aaacgaagct aaaatagtat
      781 ctaacaataa attaatttga ctaacggaca caccaaacat cgcaggcaac attaaggtaa
      841 aaatcttttt taccccttcg tccgcaaaag cgactttaaa acgtggcaac ataccaatat
      901 tgtataaagc aggaatttgt atagctaact gtgcaacacc tgccaacaac acaccccatg
      961 ccaatgccat aattggaatg tcaaactgtg gggctaaaaa caacgcactg ccaatcatca
     1021 ccaaattcaa caaaacaggc gtgaatgatg ataaagcaaa tgcgccatag ctatttaaaa
     1081 tcccacctgc aaatgccgtc agtgaaatta acaataaata cggaaaggta atttgcagca
     1141 tatctgctgc taaattaaat ttttcagcat caccactaaa accaggggca aacacccaca
     1201 taatcaatgg cgcaccaaca atcgcaataa tcgtcacgac aaacaaaatc aacgataaag
     1261 agccagccac tttactaact aaatctttca ccgcttcgtc ccctttttgg cttttatact
     1321 ctgacaacac aggcacaaaa gcttgagcaa aagccccttc agcaaacaaa cgccgcaaaa
     1381 agttaggaat tttaaaggcc accaaaaagg catccatcat tttgtcagca ccaaaggcgt
     1441 tcattaaaac catatcacgg actaagccta agacgcgtga caacatcgtc attgcactga
     1501 caatgagcgt ggaacgccat aaaccagact ttttagcacc ttgagtggcg gacttttgcg
     1561 aaggaggagt tacctgagac atgaaaaatg actttgctaa ttaacaaagg ggcaaatagt
     1621 agcgaattag ccgcataaaa actataaaaa tgcggtttta tacgtaaaaa gttggtaatt
     1681 atcttgactt aatgctctat gctcggcaaa atcgcacgcc tgattttggg tagtaatccc
     1741 tttagtatct cgacttatag acatccctat aagtcctgcg aacgattttt tataggagtg
     1801 ccaccgtggc caattctgca caagccaaaa agcgtgcgcg ccaaaacgaa caacaacgta
     1861 agcataatgc aagtttacgt tccatggtgc gtacttatct caagaaagtt gtaaatgcta
     1921 ttgctgctgg cgataaagct gctgcatctg ctgcttatac tgctgccgtt cctgttgttg
     1981 accgtatggc tgacaaaggt atcatccaca aaaacaaagc agcacgtcat aagagccgtt
     2041 taaacgctca tatcaaagca atggcttaat actgatttaa gtatcgcttt gtaaaaaccc
     2101 gcttagtgcg ggttttttat tgcttgacat ttaatcatta tttattgata attattctca
     2161 ttagtattta tgaatgagaa taattctatg tacgtatgtg tctgtcatgc agtcagcgat
     2221 aaacaaatta aacaatctgt agaaaatggt tgttgctctt atcgtgaaat tcgtgattgt
     2281 cataaagttg gcacaacctg tggccgttgc gtacctgaag cacgtcaatt aattgatgaa
     2341 acacttgcaa acttagccaa aaatatttca catgcagcgt aacaactcat cactctctaa
     2401 aaaccaaatg ataactattt gtttttacaa tctaatccac taagatttct tacgcttttt
     2461 cctcatttgc ccactgtctt ttatcttgtt ttacgctacc attagtgtaa ttgttgacaa
     2521 gaacaatcag ctattagcaa atctataaat atccccaccc ttcttattta aatatatcgt
     2581 ggagtaacaa catgaaaggt gatgcacaag ttattgctta tttaaacaaa gccttaacca
     2641 atgaattaac agccattaat caatatttct tacatgcacg tatgttaaaa agttggggct
     2701 ttgaaaaact cggtcaacat gaatacatgg agtcaattga tgaaatgaaa catgctgaca
     2761 aattaattga ccgtatttta tttttggaag gtttgccaaa tctacaacac cttaataaat
     2821 tatatattgg tgaaaatatt gaagagattt tacaaggtga cttgaaactc gaacaactcg
     2881 cccaccccga tttaaaggat gcgattgccc attgtgaaaa agtaggtgac tacggctctc
     2941 gtgaattatt tgtagccatt ttagaaagcg aagaagaaca tattgattgg attgaaaccc
     3001 aattagaatt gattaataaa ctgggtgtgc aaaactattt acaatcagct atgtaataga
     3061 caaaaaaaag cccactttgc agtgggctaa caggggttca accagagaga attatcaaga
     3121 gctagtagat tcatcgccac tcacctacta aatcttgata ccatgtactc aaagcctaat
     3181 atacgctttt atttaattta cactgttatt gagatgtgtc gtttatgtga atgaactgtt
     3241 aacttggttc atttaatttt aaacgattac gatttttttg tttcattggc cacgtcatca
     3301 caaactttgc tccacccaaa cgtggacttg cttctacttt tatattgcca ccaaaccaaa
     3361 aagcaattct tgcgacaata gataatccca aaccataacc accagaagcg cgggtacgac
     3421 tatcatctaa acgagtaaac ggctgaaaca ctttttctcg gtctttttcg ggaataccat
     3481 gtccatcatc ctcaaccgca atataagcac atccttcata aatacccgca cttaaacaca
     3541 cttcactttt ggcataacgc gtggcattac ctgccaagtt ttgcaataca cgatgcaaat
     3601 aacgacgctc agcttccgca attgtgcctt gtggaggcaa ctgaacagtc actttttgtt
     3661 taacgcctaa agctgttgtt tctttggcaa ctttttgtaa taactcgcct aaatcaactg
     3721 cctcaaaatc taaaacagga atgccctgct ctaatttggc ataggttaga atttcgtcaa
     3781 tcagtttatt aagctcatca atatcaccat ctaactgcgc taactgccga aagcgatcct
     3841 caatatcatc ggtatcggcc aacatctcca taccaaaacg aatacgggca acaggtgttc
     3901 ttaattcatg agaaactgct cttgtcaact cgcgttgaga atcaatcaag cgttgaatat
     3961 gttcagccat actattaaaa gcatacgcta actgactcac ctcgtcctta ccatcaactt
     4021 cagcacgcgc acttaaatca ccgccaccaa tacgtctcgc agcactctcc accgttttaa
     4081 ttttcttttc taagggatga ataatgagat atgccgttgt cgtaatgact aataaagcaa
     4141 ttgccgaaat aatcaataat aattgaacag gcatccaatc gaataaatac atggggccaa
     4201 tgactacaac ctgattaaac tgagatgaag gcgcaataat tcgtacagaa gaattatggc
     4261 caacgccacc ttctttcaga gccaaaacta cttctttacg tttaatacgt gcgcgttgat
     4321 cgggttctaa actagcctta tccaaaggca ttatgtgtaa aggaaaagga aaataaactt
     4381 gcaattgttt taatcttttt tgctcttgac ctcgataatg atctaagtca tccaacaaaa
     4441 aaatcgccat tgctttggct tgttgctctg agactttgct catgcgtact tgtaaatatt
     4501 gatctttttc aacagagact gcgctataaa tatctgcata actaaaatta ttagcaattc
     4561 tgacaacggc cttaccctgc tccagttgtt ctaattctcg actggtaaaa tcaatctgtt
     4621 gagctttgct taaaacaatt ttgctatcta ataaagtact tgcttcttca atccatgctt
     4681 gttgcaactc tggactagat tgacgtgcaa ttcccaaggc aattaaacga aaaaagcccg
     4741 ttgccatgct ttcgcggtat tctgcggcac ggtaatcatt aatcacttgt aacagtaaat
     4801 aggtacacaa ggcgacggca ctgatgatta acaacaggcc gccataaatg cgaacaaaaa
     4861 tactgctagg aattggcagc ttaggcaaac taaccgccta aagactcttt aacaaataaa
     4921 tagcctttac tgcgtaccgt tttgatacgt ttaggattat caggatcatc accaattttt
     4981 ggacgaatac gggaaatacg cacgtcaatc gaacgatctt gtccatcata ttcaatgcca
     5041 cgcaaacgtt caaagatgtc ttcacgcgat aaaatacggc cagcattaga ggctaataac
     5101 cataacaaat catattcggc actggtaaac tcaacaggct catcagctaa agtcactgaa
     5161 cgaccgccat tatcaatcac taaatcatca aactctaagc gttgtggcac atcttcttcg
     5221 acaattttat cggtacgacg taacaaggca cgaatacgtg ctaataacac acgtggttga
     5281 gcaggtttgg caacataatc atctgccccc atttctaagc ccaacacctg atccatatca
     5341 tcagtgcgtg cggttaacat taaaattgga tgatggtaat gtggacgaac ttcacggcaa
     5401 atggttaaac catctgcacc tggtaacatg acatctaaaa cgactaaatc gggttgttcg
     5461 gcaataatac ggcgaatcgc acgattacca tccgtttcta tcgcaaccaa taaaccatta
     5521 cgaattaaat actcttgcgt caacgttgct aaacgttcgt catcttcaac aattaaaatg
     5581 cgtggcaaat gttcttcttg ttggctcatg gtgttttcct actaaactaa taatcttaaa
     5641 tgttgtaacg acgtagcctt ttaattgaca gttacattgt aaatcctgtc gctacgcgcc
     5701 atacctgcat aatagacaat tctgtgtcag ttacaaagtg acaaatgcca tttgtttacg
     5761 aacataccac taaaagcctg tcaaggctaa aaatcagata tattttttta catgtcattt
     5821 acgtttagtt atccacaagt aaaagttatg caaccctttg aatcacttgt tgtcttgcgc
     5881 ttttgattgt tttttaacag tagttatcca cagttttgcc ctcttgcata ttctaaaaaa
     5941 agagcttatc ttgtgccgaa attatgaaat acacacaata gcttgtgttt taagcttttt
     6001 cttacactaa atatataaat aaatcaattc ttaactgatg ctttttatga ggccgcagac
     6061 ggggattttt ttgtttggta aagccaccta gggagcttat atgaccattt ctgcacaacg
     6121 ttcttttgag tcaacagaac cattagccac cgcgccaggt gtattacgtg ttattaaacg
     6181 taatggcact ttagtcactt acgatgctag caaaattgct attgccatca gtaaggcttt
     6241 tttagctgtt gaaggtggct ctgctgctga ttcaagccgt gtgcatcaac aagtagcgtt
     6301 attaaccgat gccgtttcta atacttttaa acgtcgtatg cctagtggtg gcactgttca
     6361 tattgaagaa attcaagatc aggttgaatt agcattaatg cgtgctggcg agcaaaaagt
     6421 ggcgcgttct tatgtgattt atcgtgatga acgcgctcgc ttgcgtgatg aaggcatcac
     6481 taaaagccat ccaaccttaa ccgtgacgtt gggtgacggt acacgtcagc ctttagattt
     6541 aggtcgttta gaggctttag tgagcagtgc ttgtgaagat ttaacaggcg taaatgcgca
     6601 agcaattgtt gatgaaactt taaagaattt gtatgacggc gttaaagaaa gcgatttatg
     6661 taccacaatg gtgatgtgtt ctcgtgtaca tattgaacaa gagccaaact atacctatgt
     6721 gactgcacgt ttattattag atcaattacg cagtgaagca ttaagcttct tagatattgc
     6781 tgataaagcg tctcaagccc aaatgtcaga tttttatcct caagccttac ccgtattttt
     6841 aaagcgtggt gttgagttag agctgttaca ccctgactta ttaaaatttg acttagccaa
     6901 attagctgat gcgattgagc cagagcgtga ttttcagttt acctatttag gtttacaaac
     6961 cctttacgac cgttatttta ttcatagcaa cgaagtccgt tttgaattgc cacaattatt
     7021 ctttatgcgt gtggcgatgg gcttagccat gaatgaagcc aacaaagaag cacgtgcgat
     7081 tgagttttat caattgttgt ctagctttga ctatatggca tcgaccccta ctctctttaa
     7141 ctctggcact ttacgcccac aattatcgag ctgctactta accactgtgc ctgatgattt
     7201 gtatggcatt tatgatgcga ttcgtgacaa cgcgatg
//
LOCUS       DAOQMB010000073         7225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4261_length_7225_cov_15.478940, whole genome shotgun sequence.
ACCESSION   DAOQMB010000073 DAOQMB010000000
VERSION     DAOQMB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4261_length_7225_cov_15.478940"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="PLY05_04120"
     CDS             complement(<1..938)
                     /locus_tag="PLY05_04120"
                     /inference="COORDINATES: protein motif:HMM:NF011034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC02523.1"
                     /translation="MYLADFHQHLSQLGAQFIHRQRILRAWLDARPLDSGACRRDTPE
                     FLSLAVRQDLPNLAKKLDNLARVRSEHPAADGSVRLLIDLPDGQMVESVMLPRDGVCI
                     SSQVGCAVGCRFCMTGKSGLLRQVTSLEMVAQVVQARRRRPVKKVVFMGMGEPAHNLD
                     NVLEAIDILGTDAFIGHKNLVLSTVGDMRVFDRLPTLKVKPALALSLHTTKADLREYL
                     LPKAPKISPQELMELAESYARLVKHPTQVQWTLLKGVNDGQDELDAILKLFKGKYAVL
                     NLIPFNSLEGDDYQRPDGERIVEIVRYLHSRGVLTKV"
     gene            complement(981..2654)
                     /locus_tag="PLY05_04125"
     CDS             complement(981..2654)
                     /locus_tag="PLY05_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC02524.1"
                     /translation="MNLLALSQQLQQHFSWQNVEIKPLGDVNSYFNQTFKVLADGQAY
                     ALQCVPTQSAVFAQNAYLQLPQVLAALNQNHCKQWYPHRYLEMIATQNGDYYLPSQIN
                     GQEVALRLFKWIDSTAIAQVTETIAFEAGKILADFHLAVQDVKGLNDLVEHAIEPLPH
                     FHDTAWYFQHWQQAVTNNQWTDNSLDLSEHLATIEQGVDLIKQFDVFRQQQLQQQPRI
                     MHGDPKLANMLFEQEQAVALIDFDTIMTGLWLYDVADALRSLCNTQPENGDINQVTFD
                     LNLFSAFMRGYAPALLAWTDQEKQMLSSAISLLPLELGIRFLFGCHQDHPLRLEAQPQ
                     AAAHKALVQLRLFQDIKNKQPQIDHILQQKMPKALITHPQHPQSPPVTINTVIRQNAQ
                     QLEVFYRVDSQQRLDDCLAAVPVVKIAENQAKGLWQHNCLEVFIANKNQSAYLEFNGA
                     STQQSDVFVFSAERVNGQGVKTSEHILPTCATQAQWQWFYAQTTNNQALAKFSLDLTS
                     VADVIDTTQPIEYSVTAVVEDRKGQHYYLALQHCRSEADFHARESFGRV"
     gene            2770..3933
                     /locus_tag="PLY05_04130"
     CDS             2770..3933
                     /locus_tag="PLY05_04130"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004726519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNC02525.1"
                     /translation="MSTNFPADSVGLVKPQRADFNQPLTLACERTLEAYTLMYETYGT
                     LNADKSNAILICHALSGHHHAAGYHSIDDKKAGWWDSCIGPNKAIDTNKFFVVALNNL
                     GGCHGSTGPSSFNPATGRLYGPDFPLMTVKDWVQSQARLMDYLGISCWAAVIGGSLGG
                     MQVLQWTVDFPERLRSAAIIASAPKLSAQNIAFNEVARQAILSDPDFHGGRYYEQDTY
                     PKRGLILARMVGHITYLSDDAMKQKFGRDLKTGKFLYGFDVEFQVESYLRHQGEIFSK
                     NFDANTYLLMTKALDYFDPARDYNDDLQAALSKASCKFLIISFTTDWRFSPERSQEIV
                     NALIAQGKDVIYADIDAPQGHDSFLFPIPRYVTLLQNFLNHVQHEIEDYRHAS"
     gene            3923..4513
                     /gene="metW"
                     /locus_tag="PLY05_04135"
     CDS             3923..4513
                     /gene="metW"
                     /locus_tag="PLY05_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNC02526.1"
                     /translation="MRLDLRLAERWIKPKSRVLDLGCGEGELLAHLRDNFAIRGYGLE
                     IDEDKINACVARGVSVIQQDLNKGLGNFADNSFDSVIMTQALQAVEQPDKLLKDMLRV
                     GQEAIITFPNFAHWKTRGYLFFKGKMPMSEALPYSWYNTPNIHLCTFNDFEELCEALG
                     IRILNRLAVDDEQKRSFLTKRFPNILGEVAIYRVSR"
     gene            4514..5434
                     /locus_tag="PLY05_04140"
     CDS             4514..5434
                     /locus_tag="PLY05_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNC02527.1"
                     /translation="MAIPDFQSLMLPLLNFCASGTEVKNSDATAYLGQLFRLTEHELQ
                     QKLPSNRQKVFDNRVAWAKAYLKMAGLLEATQRGVFRITAEGQRLLSAPPPLLNLAFL
                     QTIPAYQYAKSSRLPEALSPLRPVRDTQIQTPHEIIETAYQDLSFRLADELLDKIKNN
                     TPAFFEKLVVELLLKMGYGGTRADAGAAIGKSGDEGIDGIIKEDRLGLDAVYIQAKRW
                     KDTVGRPEIQKFVGALHGQRAKKGIFITTSRFSQEAEDYVAKIDNKIALIDGKSLCQL
                     MIEYDIGVATEISYAIKRIDSDYFEEENSL"
     gene            complement(5431..6363)
                     /locus_tag="PLY05_04145"
     CDS             complement(5431..6363)
                     /locus_tag="PLY05_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC02528.1"
                     /translation="MFAWQMTANAWQGNEEGTFLKAFLYYQSYILPFFLALVLWGFKH
                     KQVSIGFMLIAVIGCVLGIYARFIEPNLLLVKETTIKTGYSLKIALISDMHYGLYSTP
                     WQMQRLVNKLNTLEVDMLLVAGDWTYEPAKNSSLTEQLAPFKQLKHPIYSVPGNHDEE
                     MPGPPLAKELKQALIANNIRPIEATTIDLGKVRLVGLADLLPLATKEIRLNALKQQDK
                     PLLLLTHNPESLEYLPKLTQPFVMLAGHTHGGQVNLPILTEKILHLLTEQGYKRGMYP
                     LENNNQLFITSGIGMVGLPLRFAMPPTIDILNFY"
     gene            6536..7138
                     /gene="rdgB"
                     /locus_tag="PLY05_04150"
     CDS             6536..7138
                     /gene="rdgB"
                     /locus_tag="PLY05_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HNC02529.1"
                     /translation="MQKLVIATGNQGKLHELQSLLKDYPYELVTQKSLGITDADETGL
                     TFVENALIKARHAACISGLPALADDSGLCVDVMNGGPGIYSARYAGAGAKDSSNNEKL
                     LNDLLPFRGQSPLKAQFVCVLVFLRHANDPLPIICQGIWHGEILPEKRGEHGFGYDPL
                     FWLPERGCSSAELEPSLKNQISHRGQAMSQLKAALATCEF"
BASE COUNT         2129 a         1456 c         1449 g         2191 t
ORIGIN      
        1 actttggtta acacaccacg actatgtaaa tatcgtacaa tttcaacaat acgctcacca
       61 tcaggacgct gatagtcatc cccttctaaa ctattaaagg ggattaaatt aagcaccgca
      121 tatttgcctt taaaaagctt taaaatcgcg tctaattcat cttggccatc attgacacct
      181 tttaataatg tccattgtac ttgggtggga tgtttaacta aacgtgcata agactcagct
      241 aattccatca gctcttgggg agaaattttg ggtgctttgg gtaataaata ctcacgtaaa
      301 tcggctttgg tggtatgtaa agacaaggca agtgcaggtt ttacttttaa tgtaggcaat
      361 ctatcaaaaa cacgcatatc acccacagtt gataacacaa gatttttatg accaataaac
      421 gcatctgtgc ctaaaatatc aatcgcttct aaaacattat ccaaattatg tgcaggctca
      481 cccatgccca taaaaaccac ttttttgact ggccgacggc gacgagcttg caccacttgc
      541 gcgaccattt ctaggctggt gacttggcgc aataaaccac tttttcccgt catacaaaat
      601 cgacagccaa ccgcacaacc cacttggcta gaaatacaca cgccatctcg cggcagcata
      661 acactttcga ccatttgacc atcgggtaaa tctatcaaca aacgcaccga accatctgcc
      721 gcaggatgtt cggaacggac tcgtgccaag ttgtctaatt ttttagccaa attgggtaaa
      781 tcttggcgta cagctaaaga taaaaattcg ggcgtatctc ggcgacaagc tcctgaatct
      841 aaaggacggg catctaacca tgcgcgtaga atacgttgac gatgaataaa ttgtgcgcca
      901 agttggctaa gatgttggtg aaaatcggct aaatacataa tctatttgaa gttggggact
      961 aaaacatccc ctattctacc ttagacgcgg ccaaaagact ctctggcatg aaaatcagcc
     1021 tccgaccgac aatgttgtaa tgccaaataa taatgttgcc ctttcctgtc ttcgacgacg
     1081 gctgtgactg aatattcaat tggctgtgtg gtatcaatca cgtctgcaac actggttaaa
     1141 tctaaactaa acttagccag tgcttgatta ttggttgttt gtgcataaaa ccattgccat
     1201 tgtgcttgcg ttgcacaagt gggcaaaatg tgttcagatg tttttacccc ctgtccattg
     1261 acacgctccg cactaaacac aaatacatca gactgctgag tgcttgcacc attaaactct
     1321 aaataagcag actgattttt attggcgata aatacttcta agcaattatg ttgccataat
     1381 cctttagctt gattttctgc gattttaact acgggtactg cggctaaaca gtcatctaag
     1441 cgttgctgac tatcaacacg ataaaatact tctaactgtt gagcgttttg tctaatgact
     1501 gtgtttatgg taacgggtgg tgattgtggg tgttgaggat gcgtgattaa ggctttcggc
     1561 atcttttgtt gcaaaatatg gtcaatttgc ggttgtttgt ttttaatatc ttgaaataag
     1621 cgtaattgca ctaaagcttt atgagcggcg gcttgtggct gcgcctctaa acgcaaagga
     1681 tgatcttgat gacaaccaaa caaaaagcga atgcctaact ctaaaggcaa taagctaatt
     1741 gcggagctta acatttgttt ttcttgatct gtccatgcta acaatgcagg ggcgtaacca
     1801 cgcataaacg cactaaataa atttaagtca aaagtaactt gattaatatc accattttcg
     1861 ggctgcgtat tacacaacga gcgtaaagca tcagccacat cataaagcca taagcctgtc
     1921 ataatggtat caaaatcaat cagtgcaaca gcttgttctt gctcaaataa catattggct
     1981 agttttggat caccgtgcat gatgcgtggt tgctgttgca attgttgctg tctaaaaaca
     2041 tcaaactgct taattaagtc aacaccttgt tcaatcgtgg ccaaatgctc acttaaatct
     2101 aggctattat ctgtccattg attgttagtg actgcttgtt gccaatgttg aaaataccaa
     2161 gcggtatcat gaaaatgtgg caaaggttca attgcatgtt caactaaatc atttaagccc
     2221 ttaacatctt gcacagccaa atgaaaatct gccaatattt tacctgcttc aaaagcgatg
     2281 gtttcggtca cttgtgctat agctgtgcta tcaatccatt taaataatct taacgccact
     2341 tcttggccat taatttgact aggtaaataa taatcgccat tctgggttgc aatcatttct
     2401 aaatagcgat ggggatacca ttgtttgcag tgattttgat ttaaggccgc taaaacttgt
     2461 ggtaattgta aataggcatt ttgagcaaaa acggcagact gcgtaggcac acattgcaga
     2521 gcataagcct gaccatcagc caatacttta aaagtttgat taaaataaga gtttacatcg
     2581 cctaaaggct taatttcgac attttgccac gagaaatgct gttgaagttg ttgggagagt
     2641 gctaagaggt tcataatttt atccatttat attactttgc taaattacca tatattctat
     2701 gcccccttgc actccctccc tacactcttt agactaaacc tcccaaaaac cgccaatatt
     2761 gaacatccaa tgtccacaaa ctttcctgct gactctgtgg gtttggtaaa accccaacgt
     2821 gctgatttta atcagccttt gaccttagct tgcgaacgta ccttagaggc atacacctta
     2881 atgtacgaaa cttatggcac gctcaatgcg gataaatcca atgccatttt gatttgtcat
     2941 gccttatcgg ggcatcatca tgcagcgggt tatcacagca ttgatgacaa aaaagcaggt
     3001 tggtgggata gttgtattgg ccctaataaa gccattgata ccaataagtt ttttgtggtc
     3061 gctcttaata atttaggcgg ctgtcatggt tcaacaggcc cttcttcgtt taatcctgcc
     3121 actggccgtt tgtatggccc agactttccg ttaatgaccg ttaaagattg ggtacagtca
     3181 caagcacgat taatggatta tttaggtatt agttgttggg cagcggtgat tggtggctca
     3241 ttgggcggca tgcaagtttt acaatggaca gtggatttcc ctgagcggtt acgcagtgca
     3301 gcgattattg ccagcgcacc caaattatca gcacaaaaca ttgcctttaa tgaagtggct
     3361 cgacaagcga ttttatctga cccagacttt catggtggac gttattacga acaagacacc
     3421 taccccaaac ggggtttgat tttggcgcga atggttggcc atattaccta tttatctgac
     3481 gatgccatga aacaaaaatt tggccgtgac ctaaaaacag gtaagttttt gtatggtttt
     3541 gacgttgagt ttcaggtgga atcttatttg cgtcatcaag gcgaaatatt ttctaaaaac
     3601 tttgatgcca acacctattt attaatgact aaagctttag attattttga tcctgcacgc
     3661 gattataacg atgatttaca agcggcatta agtaaagcaa gctgcaagtt tttgattatt
     3721 tcgtttacca ccgactggcg tttttctccc gaacgttcac aagaaattgt taatgcttta
     3781 attgctcaag gcaaagatgt catttacgcc gatattgatg caccgcaagg ccatgattca
     3841 tttttgtttc ctattccacg ttatgtcacc ttattacaaa actttttaaa ccacgttcaa
     3901 cacgagattg aggattatcg tcatgcgtct tgatttacgt ttagcagagc gttggattaa
     3961 acctaaatct cgtgtgttag atttgggctg tggcgaaggt gaattattag cccatttgcg
     4021 tgataacttt gccattcggg gttatggttt agaaattgat gaagataaaa ttaacgcctg
     4081 tgtagcccgt ggtgtgagtg ttattcaaca agatttaaac aaaggtttgg gtaactttgc
     4141 tgataacagc tttgatagcg tgattatgac tcaagcctta caagccgttg agcagcccga
     4201 taaactgtta aaagatatgt taagagtcgg tcaagaggcg attattacct ttcctaactt
     4261 tgcccattgg aaaactcgcg gctatttgtt ttttaaaggc aaaatgccca tgtccgaagc
     4321 cttaccctac tcttggtaca atacgcccaa tattcatctt tgcaccttta acgactttga
     4381 agaattatgt gaggctttag ggattcgtat tcttaatcgt ttagcggttg atgatgaaca
     4441 aaaacgctca tttttaacca aacgctttcc taatatttta ggtgaagttg cgatttatcg
     4501 agtgagccgt taaatggcta ttcccgattt tcaaagttta atgctgcctt tgcttaactt
     4561 ctgtgcgtcg ggaacagaag ttaaaaatag tgatgcaacg gcctatcttg gtcaactttt
     4621 ccgtcttaca gagcatgaat tacagcaaaa acttccctct aatcgtcaaa aagtttttga
     4681 taatcgggtg gcttgggcaa aagcctattt aaaaatggca ggattattag aagcaactca
     4741 acgtggggtg tttagaatta ctgctgaagg tcaaaggctg ttaagcgcac cgccaccatt
     4801 attgaattta gcttttttac aaacgattcc tgcctatcaa tatgctaaaa gcagtcgtct
     4861 ccctgaggca ttaagtcctt tacggcctgt tcgtgatact caaattcaaa ccccacacga
     4921 aattattgaa accgcctatc aggatttaag ctttcgattg gcggatgagc tattagataa
     4981 aattaaaaat aatacgcctg ctttttttga aaaattagtg gtcgagttgt tattaaaaat
     5041 gggctatggc ggcacacggg ctgatgctgg tgcggcgatt ggcaaaagtg gtgacgaagg
     5101 cattgatggt attatcaaag aagatagatt gggtttggat gctgtctata ttcaagccaa
     5161 acgctggaaa gacaccgttg gtcgccccga aattcaaaaa tttgtcggcg cattacatgg
     5221 tcaacgcgcc aaaaaaggca tttttattac cacctctcgc ttttctcaag aagcggaaga
     5281 ttatgttgcc aaaatcgaca acaaaattgc cctcattgac ggcaaatcct tatgccaatt
     5341 aatgattgaa tacgatattg gcgtggcaac cgaaatcagt tatgccatta aacgtattga
     5401 ttcggattat tttgaggagg aaaacagcct ctaataaaaa tttaatatat caattgtagg
     5461 tggcattgca aaacgtaagg gcagccctac catgccaata ccactggtaa taaatagctg
     5521 gttattattt tctaaaggat acattcctct tttatagcct tgctcagtca gcagatgtaa
     5581 aattttttct gtcaaaatcg gcaaattgac ctgtccgcca tgcgtatgtc ctgccaacat
     5641 aacaaatggc tgagttagct ttggtaaata ctctaaactt tcaggattat gagttaataa
     5701 cagtaaaggt ttatcttgtt gttttaaggc atttaggcgt atttctttgg tagccaaagg
     5761 taataaatcg gccaagccca ctaaacgcac tttacccaaa tctatcgtag ttgcttcaat
     5821 aggccgaatg ttattggcaa ttaaggcttg ttttaactct tttgctaaag gagggcctgg
     5881 catttcttcg tcatgattac ccggtacaga ataaatagga tgttttagtt gtttaaacgg
     5941 tgcaagctgc tctgttaatg aactattttt agcaggctca tacgtccaat cgcccgccac
     6001 caacagcata tccacctcta aggtattgag tttattgact aagcgttgca tttgccaagg
     6061 cgttgaatat aaaccatagt gcatatcact aattaatgct atttttaagg aatatcccgt
     6121 ttttatggtt gtttctttca ctagcaacaa attaggctca ataaaacgcg catagattcc
     6181 taaaacacag cctattacag caatcaacat aaaacctatc gatacttgtt tatgcttaaa
     6241 gccccaaagc actaatgcca aaaaaaaggg caaaatatag ctttggtaat ataaaaacgc
     6301 ctttaagaat gttccctctt cattaccttg ccacgcattt gctgtcattt gccatgcaaa
     6361 catcgtcaac accatcatga atatgacgag taatcctaaa taaatatgct gctttttagc
     6421 cacgtttgct gctctaaaaa aattaatgat gtctgagtct tgttaaaaac agtcgattaa
     6481 agaaatctgc tactatagat gccctcgcac gacatgataa gacttgggat attgtatgca
     6541 aaaattggtc atcgccacag gtaatcaagg taaattacat gaattacaga gtttgttaaa
     6601 agattatcct tatgaattag tcacccaaaa atcgttgggg attactgatg ctgatgaaac
     6661 aggcttaacc tttgttgaaa atgcgttaat taaagcacgt catgccgcct gtatttcggg
     6721 tttacctgca ttagccgatg attcagggtt gtgtgtggat gtgatgaacg gtgggccggg
     6781 tatttattcg gcacgttatg caggggcagg cgcaaaagat agcagcaaca acgaaaaatt
     6841 gctgaatgat ttgttgccct ttcgtggtca atcaccactc aaagcgcaat ttgtttgtgt
     6901 tttagtgttt ttgcgtcatg ccaatgatcc tttaccgatt atttgccaag gcatttggca
     6961 tggcgaaatt ttgccagaaa aacgcggcga acatggtttt ggttatgatc cgttattttg
     7021 gttgcctgaa cgaggttgta gcagtgccga acttgaaccg agtttaaaaa atcaaattag
     7081 ccatcgtggt caagcgatga gtcaattaaa agcagcattg gcaacatgtg agttttaagg
     7141 agatattcaa ttgcaaggat gcaaaaaatc actccaacac tgcattgact ttcgataact
     7201 ccaccaaaaa ctcgtaggcg gattg
//
LOCUS       DAOQMB010000074         7225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4262_length_7225_cov_7.962343, whole genome shotgun sequence.
ACCESSION   DAOQMB010000074 DAOQMB010000000
VERSION     DAOQMB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4262_length_7225_cov_7.962343"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="PLY05_04155"
     CDS             complement(<1..648)
                     /locus_tag="PLY05_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02530.1"
                     /translation="MPEVTWSQDEFSRGELSPMIYGRVTIDASQKGVKKGLNTITYPQ
                     GGIGKRFGTIYQGEITGVTDWKDIFFESFPYLNECVYLLVFVPGQLEIYLEGFLVATV
                     AAPSIISEVIRTMDWTILDKYFRITQNRIQPKDLTRSSLAANPINAGAGIIANQFTMT
                     NVIAPNIISAARFTTTGTLPTTTPQIITDRTYFIRTDATGFLIKVYNTAADASADI"
     gene            complement(657..1247)
                     /locus_tag="PLY05_04160"
     CDS             complement(657..1247)
                     /locus_tag="PLY05_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02531.1"
                     /translation="MITKTSIISNAVTQLGHAPVVSLIDQDDLIVAAEQAYDMLLPSV
                     LSQGNWRFATKVEQLSQLVEIPPEPYKYVYQLPSGWLKTLSVWPNTYDWDIFHGNKIY
                     TFQDTSWFMQFIFQPDVSLLPPWFVSSFVYEISTYLCLSNAEKTDYYAVLERKRVEMQ
                     ALAMGVDCQNRPQFTQVDFPVLGNRYIGGVYPNSLS"
     gene            complement(1268..1924)
                     /locus_tag="PLY05_04165"
     CDS             complement(1268..1924)
                     /locus_tag="PLY05_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02532.1"
                     /translation="MAYNDTRWTRQTLAFNSGRVVTDQGPENGPALFSYASAADNIAT
                     ITAANYFAPAVYDLAVGDIIFIEASDADGIYLVDAVDRNAETVSVVSYGPVGAVGTAN
                     LIDGAVTALKLASDAVTTVKILNANVTLAKLAAGIAPSHVVKYAAEYTTTGGSASEAI
                     TVNGVLATDLVFVQVKNDGTNNRTVLQAAATLNTITVTFSGDPGADLVLYYQVLRAAV
                     "
     gene            complement(1939..2769)
                     /locus_tag="PLY05_04170"
     CDS             complement(1939..2769)
                     /locus_tag="PLY05_04170"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HNC02533.1"
                     /translation="MSTSLTNVQQIEFDALVKAEYRSQGFLLQNSVRMKYDVIGAQVE
                     FRKVNQVISVPTAYLAAVTIQDPGYNKVLCTLQKYTTPTAVDEVQELTVNFDAKMENA
                     MLVAQAMGRRSDQITIDAIAADVGDTIPDGGTNFNYEKFTQCLEFFDNHAVPLAERYV
                     AMSASNFKSLMQDDQFTSTFYTRNDPIDRARIREYLGFNVVIIPQMTEGGLPQVGNIQ
                     TALAWHKMSTGMGIGMNFRTEINYIPQNTSYLVNGVFSAGAVVIDNRGTLAIECDLTA
                     "
     gene            complement(2912..3616)
                     /locus_tag="PLY05_04175"
     CDS             complement(2912..3616)
                     /locus_tag="PLY05_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02534.1"
                     /translation="MTFDTISATVEDMAAQEAQPVSTPSEPAWWLDDNTPGVGERPEW
                     LPSQFKKASDVAKSYQELQKRFGEAPSEYSWEAGKGWVEPDYEPFQELAQFAKSKRVP
                     QDVMDKMLSTVGKYLDEFSIDYEAEKQALGEKADERLDILNNWARSNLSEQSFQALTT
                     NLRTADAVLALEELRSKMLGQNTMIPGNEQAQSDGVFSLEDLQAELTQNIDKYKSDPR
                     YRREITEKIERLQNNR"
     gene            complement(3616..3921)
                     /locus_tag="PLY05_04180"
     CDS             complement(3616..3921)
                     /locus_tag="PLY05_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02535.1"
                     /translation="MSNPYIEPHNYYEEYQTTEQPKEGIALDELCWYVFSTDDGKKLL
                     EIMKERFILTATPGPINENYPNMCVFYEGFREAFRQIIGSVQTYQIKKDNEAKKAGV"
     gene            complement(3923..5497)
                     /locus_tag="PLY05_04185"
     CDS             complement(3923..5497)
                     /locus_tag="PLY05_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HNC02536.1"
                     /translation="MAELLDQFKQRYNRAMQISYLWASLHEACYFYAIPNRNRFWRPK
                     EQQGEAKGTRVYDTTAIEATKTFVSKLHTAMTPPQTQWGFLSIDPEFDEDDAGISRDD
                     AQRLLNDYMRKLFDYIHDSNFDVVINECYFDLAVGTSCLVINQYTDDQPLLFTSIPMD
                     KLAIEEAMTGKIESWYRNWEDVKANEITVRWPKAVLPDELKREIKNNPDYTIKIVYEG
                     VMYNPQNTKKYQYVVCSDNNVFYTEDFEVNPGIVWRFQKTNADTYGRGPIMDALPSII
                     SLNELARIELAAANLNTFKPYMAFSDAVFNPHTFKMQPMTVIPIAPLGSSGQVPLIPL
                     PDTSNPQFSQLSIQDLRMQIRSLLFADSLIPTDSKQPASATEVMIQKQSLAERIGPLF
                     SRLQQEFLWPVIERCSYILDKMGLLPYPQIDRKMISFVYRSPLALAKGQEQIARFTQY
                     FQLLQGISGPEAAQIFINPMEYPYLLADLMQIDNRLLNAPAEVQQTMQAQQDKRNEMQ
                     DMMMQQGAMQPQQPQV"
     gene            complement(5501..5785)
                     /locus_tag="PLY05_04190"
     CDS             complement(5501..5785)
                     /locus_tag="PLY05_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02537.1"
                     /translation="MSWLSKGLKSLEGAVASVIPHQTAAERRMKSDMISAYYSQKENA
                     MAEQQRIGAEKQAQQRRIQEKTIRAMRRQYRSSGFMQPMTDASGMQSKLG"
     gene            complement(5892..6467)
                     /locus_tag="PLY05_04195"
     CDS             complement(5892..6467)
                     /locus_tag="PLY05_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02538.1"
                     /translation="MNKLDPKKIYESIKSNKSNYEEKKHCLMVLDIIGNGGTVAEFCT
                     QALISDTTYYRWRRTHTMFDNCSRIANMYAQMLWEREGEENADNPDFNWRYWEGIGSS
                     RFNHGKQARIRLNIDESADPYTQYKQLVQQAGEGDLTASEVKQLMESINIGIRSYESF
                     KLQGEVDKMKEDLVKMSVENGNNIIPIAGIA"
     gene            6519..6989
                     /locus_tag="PLY05_04200"
     CDS             6519..6989
                     /locus_tag="PLY05_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02539.1"
                     /translation="MIDNKQLRELIIKPSLDLLQMYSAEAEELLVFTCACESLGGTYL
                     KQIKGPALGIYQMEPATYTDIWQNYIKNHGMVVNLLTLNFACHNMPLPERMIYDLRFA
                     TAMARLHYRRRKEPLPKATDIDGIWEYYKKFYNTHLGKAEKESSIKHYQKYIKS"
     gene            complement(6958..7170)
                     /locus_tag="PLY05_04205"
     CDS             complement(6958..7170)
                     /locus_tag="PLY05_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02540.1"
                     /translation="MSKMEWYQENIRCYVDSNGAHNFTPVSFVKSAHLMQATMLMCTH
                     CLQIIELKDVQEFNHEATTLCTSDNA"
BASE COUNT         2059 a         1442 c         1345 g         2379 t
ORIGIN      
        1 gatatcagca gaagcatcag cagcagtgtt atagacttta attaaaaagc ctgtggcatc
       61 tgttctaata aaataagttc tgtcagtaat aatttgagga gttgtcgttg gtaatgtacc
      121 cgtagttgta aatcttgcag cagatataat gtttggcgca ataacgttcg tcattgtaaa
      181 ttggtttgca attataccgg ctccagcatt tatcgggttt gctgccaaac tagaacgtgt
      241 caaatctttt ggctgtatac gattttgagt aatacgaaaa tatttgtcta aaatcgtcca
      301 gtccattgtt ctaatgactt cactaattat actaggcgca gcaacagtgg ctacgagaaa
      361 tccttccaag taaatttcta gttgaccggg aacaaagact aataagtaaa cgcattcatt
      421 tagatatggg aagctttcaa aaaaaatatc tttccagtca gtaacgcctg ttatttcgcc
      481 ttggtatatc gtgccaaaac gtttacctat tcctccttga ggatatgtaa ttgtatttaa
      541 accttttttt acaccttttt gagaagcatc aatagtcacc cgaccataaa tcataggtga
      601 taattcgcct ctactaaatt catcttgcga ccatgtgact tctggcatat caattcctat
      661 gatagagagt taggataaac accacctatg tatcgattgc ccaatacagg aaaatcgacc
      721 tgagtaaact gaggtctatt ttgacaatct acgcccattg caagtgcttg catttcaaca
      781 cgctttcttt ctaatacagc gtagtagtct gttttttcag cattacttaa acataagtaa
      841 gttgaaatct catatacaaa agaactaaca aaccatggtg gcaataaaga aacatccggt
      901 tgaaatataa actgcataaa ccaacttgta tcttgaaacg tataaatctt gttgccgtgg
      961 aaaatgtccc agtcataagt gttaggccaa acacttaaag ttttcaacca gccacttggt
     1021 agttgataaa catatttata aggctctggg ggtatttcta ctaattggct taattgctcc
     1081 acctttgtag caaatcgcca gtttccttgt gaaagcacac ttggcaacaa catatcatat
     1141 gcttgctcag ccgcaacaat taaatcatct tggtcaatta aggaaacaac aggagcatgg
     1201 cccaactgtg tcaccgcatt agaaataatc gatgttttgg taatcatact cctgcgctcc
     1261 ttaattatta aacagcagct cttaagactt ggtagtacag aactaagtca gcaccggggt
     1321 ctccagagaa agtaacagta atggtattca acgttgcagc agcctgtaaa actgtgcggt
     1381 tgtttgtacc atcatttttg acttgtacaa ataccaagtc agttgctaac acaccgttaa
     1441 ctgttattgc ttcagaagca gaaccgccag ttgttgtata ctcagcagcg tacttgacta
     1501 catgactagg agcaattcca gcagctaact ttgctaatgt cacgttagca tttagaattt
     1561 taacagttgt aacggcatca cttgctaatt ttaatgctgt gacagcacca tcaattagat
     1621 ttgcagttcc tacagcacca acaggaccgt atgatacgac tgatactgtt tctgcgttac
     1681 ggtcaacagc gtcgactaaa taaataccgt cagcgtcaga agcttcgata aaaataatgt
     1741 cgccaactgc taaatcatat acagcaggtg caaagtagtt agcagcagta atggttgcaa
     1801 tgttatctgc agcagaagca tagctaaata atgcaggacc attttctggg ccttggtcag
     1861 ttaccacacg ccctgagttg aatgctagag tttgtctagt ccatcttgta tcgttataag
     1921 ccatttcaat tccccttatt atgcagtgag atcacattca attgccaatg taccccggtt
     1981 atcgataact actgctccag cagaaaacac accgtttact aagtaagaag tgttttgagg
     2041 aatgtagtta atttctgtac ggaagttcat gccgataccc atgccggttg acattttgtg
     2101 ccaagctaaa gctgtctgaa tgttaccaac ttgaggcaag ccaccttctg tcatttgagg
     2161 gataattaca acgttgaatc ccaagtattc acgaatacgt gcacggtcga ttgggtcgtt
     2221 gcgtgtatag aaagtactgg tgaattggtc atcttgcatc aatgatttaa agttgctagc
     2281 tgacattgct acgtaacgtt cagctaaagg aactgcatgg ttatcgaaaa actctaaaca
     2341 ttgagtgaat ttttcatagt taaagtttgt accgccatca ggaatggtat caccaacgtc
     2401 agcagcaata gcatcaatgg taatttggtc agaacgacgt cccatagctt gagcaactaa
     2461 catggcgttt tccattttag cgtcaaagtt tacagttaat tcttgaactt cgtcgactgc
     2521 ggttggtgtg gtgtattttt gtaaagtaca taatactttg ttgtaaccgg ggtcttggat
     2581 ggttacagca gctaagtaag ctgttggaac ggaaataact tggtttactt tacggaattc
     2641 tacttgcgca ccaataacgt catatttcat acgaacggaa ttctggagta agaaaccttg
     2701 tgagcgatat tctgctttta cgagtgcatc gaactcaatc tgttgcacat tggtcaatga
     2761 tgtagacata atagtcccct ttaaaattag ttaataaata aacaactcgt tttaaattgg
     2821 gcttgtattt gtctggttgt ccgatgtggg ccgtaaatgc aagttttcca atttctccag
     2881 aaaactggac acttgcattt agtttaaaga tttatcgatt attttgcaat ctttcaatct
     2941 tttcagttat ttctcttcga taacgtggat cagatttata tttatcaatg ttttgcgtta
     3001 actctgcttg caaatcttca agactaaata cgccatcaga ctgcgcttgt tcgttaccgg
     3061 gaatcattgt attttgtcct aacatttttg acctcaattc ttctaatgct aaaactgcat
     3121 cggcagttcg caaattggtt gtaagagctt gaaagctttg ttcggataaa ttgcttcttg
     3181 cccagttgtt cagtatatct aaacgctcat cagctttttc gcctaatgct tgtttttcag
     3241 cttcgtaatc gatgctaaat tcatctaaat attttccaac agtgcttaac atcttatcca
     3301 tcacatcttg tggtacgcgt ttagatttag caaattgcgc taattcttga aatggctcgt
     3361 aatcaggctc tacccatcct ttaccagctt cccaagaata ttcacttggc gcttctccaa
     3421 atcttttttg caattcctga tatgacttgg caacatcaga agctttttta aattgacttg
     3481 gaagccattc aggtcgttcg ccaacgccgg gggtattgtc atctaaccac caagcaggtt
     3541 cactgggagt gcttactggt tgcgcttctt gtgctgccat atcttcaacg gttgctgaaa
     3601 ttgtatcaaa agtcattaaa cacctgcctt tttagcctca ttatctttct ttatttgata
     3661 agtttgcacg ctaccaatta tttgacgaaa tgcttcacga aaaccctcat aaaatacaca
     3721 catgtttgga tagttttcat taattggtcc gggtgtcgca gtaagaataa aacgctcttt
     3781 cataatttct aaaagctttt taccgtcatc agtactgaat acataccagc ataattcatc
     3841 taacgctatg ccttcttttg gctgttcagt ggtttgatat tcttcataat aattatgtgg
     3901 ctcaatgtag ggattgctca tattacacct gtggttgttg tggttgcata gcaccttgtt
     3961 gcatcatcat gtcttgcatt tcgttacgtt tatcctgttg tgcttgcatt gtttgctgaa
     4021 cttctgccgg tgcgtttaat aaccgattat ctatttgcat taaatcagca agcaagtatg
     4081 gatattccat tggattaata aagatttgag cagcttctgg cccactaatt ccttggagca
     4141 attggaaata ctgagtaaat ctagcaatct gttcttgtcc ttttgctaat gccaatggtg
     4201 aacgataaac aaatgatatc atttttctat caatttgtgg atatggcaac aagcccattt
     4261 tatctaggat gtatgagcat ctttcaatga ctggccataa gaactcttgc tgtagtcgac
     4321 taaataaagg cccgatacgt tctgcaagac tttgtttttg aatcatcact tccgttgcac
     4381 tagctggttg cttgctgtca gttggaatca aagaatccgc aaacagtagc gaacgaattt
     4441 gcattctcaa gtcttgaatc gacaactggc taaattgtgg attactcgta tcaggcaaag
     4501 ggattaatgg tacttggccg gatgaaccca atggcgcaat tggtatgact gtcattggct
     4561 gcattttaaa tgtatgtgga ttaaatacag catcactaaa ggccatgtat ggtttaaaag
     4621 tatttaagtt tgcagcagca agttcaattc gagcaagttc attcaaacta ataattgacg
     4681 gcagtgcatc catgattgga ccacggccat aggtatcagc attggttttt tgaaaacgcc
     4741 aaacaattcc tgggttaact tcaaaatctt ctgtataaaa aacattgtta tcagagcaaa
     4801 ctacatactg atatttttta gtattctggg gattatacat taccccctca tagacaattt
     4861 taattgtgta gtcagggtta ttttttattt cacgttttaa ctcgtctggt aatacagctt
     4921 taggccaacg aacagtgatt tcgtttgctt ttacatcttc ccaattacga taccatgatt
     4981 ctattttacc tgtcatcgct tcttcgatag caagcttatc cattggtata ctggtaaaca
     5041 atagcggttg atcatcagta tactggttaa tcactaaaca agaagttccc acagctaaat
     5101 cgaaatagca ttcgtttatt actacgtcga aattagaatc atggatgtaa tcaaagagct
     5161 tacgcatgta gtcattcaag agtctttgag cgtcatcacg actaatgcca gcatcatcct
     5221 cgtcaaactc tgggtcgatt gataagaagc cccattgcgt ctgtggtgga gtcatagccg
     5281 tatggagctt tgaaacaaac gtttttgttg cttcaatagc agttgtatca tatacacgtg
     5341 tgcctttggc ttcgccttgt tgttctttgg gtcgccaaaa gcggttacga tttggtatag
     5401 cgtaaaaata acacgcctca tgcagactag cccaaaggta gcttatttgc atagcacggt
     5461 tataacgttg cttaaattgg tctagtagct cagccatgtt ttatcctaat tttgattgca
     5521 ttccagaagc atctgtcatt ggttgcataa agccacttga gcgatattgt ctgcgcatcg
     5581 cacgaattgt tttttcttgt atgcgtcttt gctgggcttg tttttcagcg ccaatacgtt
     5641 gctgttcagc catggcgttt tctttttgtg agtaataagc gctaatcata tctgatttca
     5701 ttcttctttc agcagcggtt tgatgaggta ttacactcgc aacagcgccc tctaaacttt
     5761 ttaaaccttt tgataaccaa ctcattttat ctccttaaat ccatatatga acatagattg
     5821 ttttttcaac aaactcatgt ggcttaattt cgcggtctac aaatataatt ctatgaggta
     5881 gtttaatttg tttacgcaat gcccgcaatt gggatgatat tgttgccatt ctctacactc
     5941 attttgacca aatcttcttt catcttatca acctcgcctt gaagcttgaa gctttcataa
     6001 cttcttatgc cgatgttgat tgattccata agctgtttaa cttctgaagc tgttaaatcg
     6061 ccttcaccag cttgttgtac taattgtttg tattgcgtgt atgggtctgc gctctcatca
     6121 atgtttaaac gaattctagc ttgtttacca tgattaaatc tgcttgaacc aatgccttcc
     6181 cagtaacgcc aattgaagtc agggttatct gcattttctt caccttctcg ctcccagagc
     6241 atttgagcgt acatgttagc tattcttgag cagttatcaa acatggtatg ggttctgcgc
     6301 caccgatagt aggttgtatc tgaaattaat gcttgagtgc aaaactctgc tactgttcca
     6361 ccattgccaa taatatccaa taccattagg caatgttttt tttcttcata attgctttta
     6421 ttgcttttta tgctttcata gattttttta gggtctaatt tgttcatttt tagattctcc
     6481 tttacaatca ttatgtatag tttttataag gatttcaaat gattgataat aaacaactca
     6541 gagagttaat tataaagcca tctctcgact tgttgcaaat gtatagtgct gaagcagagg
     6601 agttactggt atttacttgc gcatgtgagt cgctaggtgg aacttattta aagcagataa
     6661 aagggccagc attgggaatc tatcaaatgg agcctgctac ctataccgat atttggcaaa
     6721 actacattaa aaatcatggt atggttgtaa accttttaac cttgaacttt gcttgtcata
     6781 acatgccgtt accagaacgc atgatttatg atttaagatt tgcgacagcg atggcacggt
     6841 tacactatcg tagacgcaaa gaaccgttac cgaaagcgac ggacatagat ggtatctggg
     6901 aatactataa gaagttttat aatacccact taggaaaagc agaaaaagaa tcttcaatta
     6961 agcattatca gaagtacata aagtcgtagc ttcgtgattg aattcttgaa catctttaag
     7021 ctcaatgatt tgcaagcaat gtgtacacat caacatcgtt gcttgcatca aatgagctga
     7081 cttcacaaaa ctcactggcg taaaattatg cgcaccattt gaatcaacat agcatctaat
     7141 attttcttga taccattcca ttttagacat acgccctccg ttacctatca atcaattacc
     7201 gacgattgta tgacataccg atgca
//
LOCUS       DAOQMB010000075         7214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4270_length_7214_cov_16.396284, whole genome shotgun sequence.
ACCESSION   DAOQMB010000075 DAOQMB010000000
VERSION     DAOQMB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_7214_cov_16.396284"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="PLY05_04210"
     CDS             complement(<1..326)
                     /locus_tag="PLY05_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC02541.1"
                     /translation="MSGKTAIIIGATGMVGKQVLAHLLASSVYKQVIAVTRKPIAAHE
                     KLKNLVIDFDKLTSALKDVKADEAFCCLGTTIKQAGTKTAFHKVDYTYSFEFAYVLKQ
                     NGCQHF"
     gene            520..1686
                     /locus_tag="PLY05_04215"
     CDS             520..1686
                     /locus_tag="PLY05_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017396625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HNC02542.1"
                     /translation="MSYTGMNFCLGETMQQLRDTVYKFAQEEIAPRAADIDKSNEFPL
                     DLWRKFGDLGLLGMTVSEEYGGVDMGYLAHVLAIEEISRASASVGLSYGAHSNLCVNQ
                     IYRNGNAEQRAKYLPKLISGEHIGALAMSETTAGSDVVSMKLRADKVEGGYLLNGHKM
                     WITNGPDAHTYVIYAKTDLSCQASKGMTAFIVERDWQGFSRSPKLDKLGMRGSNTCEL
                     VFNDCFVPEENVLGHVGGGAKVLMSGLDYERIVLAAGPVGVMQACMDIVIPYMHERKQ
                     FGQAIGEFQLIQGKIADMYTKLNASRAYLYAVAQACDRGEAARKDAAATILYCAENAT
                     QCALDAIQILGGNGYINDYATGRLLRDAKLYEIGAGTSEIRRMLIGRELFNETR"
     gene            1691..3301
                     /locus_tag="PLY05_04220"
     CDS             1691..3301
                     /locus_tag="PLY05_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008941402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HNC02543.1"
                     /translation="MPILHSQINPHSPSFLENAQALREQVSELKKVLQDVAKGGGEAA
                     VARHKARGKLTARERINAILDVGSPFLELAPLAAYQVYGKEDVPAAGIVAGIGRVGGI
                     ECVIAANDATVKGGSYYPLTVKKHLRAQTIAQDNHLPCIYMVDSGGANLPRQDEVFPD
                     ERHFGRIFFNQANMSAMNIPQIAVVMGSCTAGGAYVPSMADEAIIVKEQGTIFLAGPP
                     LVKAATGEIVSAEDLGGADLHCRTSGVTDQLADNDEHAIALARAAVSRLNRPKNIDLA
                     IRPPKPPRYSAEEIYGIIPKDPRQPFDVREIIARLVDDSEFDEFKPLYGSTLVCGFAH
                     WWGYPVGIVANNGILFSESAQKGAHFIELCSQRGIPLIFLQNITGFMVGKKYEEGGIA
                     KHGAKMVTAVACAKVPKFTVLIGGSYGAGNYGMCGRAYSPRFLFSWPNSRIAVMGGEQ
                     AASVLTEIQKGANAKRGVHWTAEEEAAFKQPYLEKYQRESHPFYASARLWDDGVIDPT
                     QTRDVLGLCISASLNAPSQETKFGVFRM"
     gene            3305..>4072
                     /locus_tag="PLY05_04225"
     CDS             3305..>4072
                     /locus_tag="PLY05_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005076587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC02544.1"
                     /translation="MLEHQIDHRGVVTLTLNRAELHNAFDDLLIAELIKTLKLYEQNS
                     KTRLLVLRSTGKSFSAGADLNWMKRVAQYSFAENKQDAEQLAELMSCLYHFPSPTIAV
                     VQGAAFGGGVGLVSCCDIAIASDKASFCLSEVKLGLVPAVISPYVIAAIGARQAQRYF
                     LTAERFDAQTAFHLGLVQQLTSADSLEQAADSLITTLLQNSPQALKACKKLIQDVDGA
                     INPQLRDMTTTLIAKLRASPEGKEGLSAFLEKRPAGWL"
     assembly_gap    4073..4172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4173..5633
                     /locus_tag="PLY05_04230"
     CDS             <4173..5633
                     /locus_tag="PLY05_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HNC02545.1"
                     /translation="FVAEELPEAIQAAQREGQNSFGDGRLLIEKLLVKPRHVEVQILA
                     DKHGHCVYLFDRDCSLQRRHQKVVEEAPAPDLSPELRAAMGQAAVKAAQAIGYSNAGT
                     IEFLVANNQFYFMEMNTRLQVEHPVTEMISGLDLVEWQLRIAAGEHLTLQQENLKANG
                     HAIEVRLYAEDPAHDFLPAIGKLNQFSYKQQHPWQRIDTGFQTGDEISPFYDPMLAKV
                     IAWGENRQLAIARLLQTLADIRISGLRHNLSYLQRVIAHPDFADSQHLATDFLVLRHQ
                     TLLNQDSPYQQAALLVAAAYYWQQQQQNISVVSNDLYSPWQNLVAWRTLGDNYSSLQL
                     VVNEQNQQLQLQKQGENFVWQQAEQQGQFSFKQDNHEFIYQDAQQRLRFQVTLDSHGL
                     QLAHEGVIWQCRLLEPYKQSQQQQNQHKGYCAPMTGRITAVLVNAEQAVTKGETLVVM
                     EAMKMEHRIVAQDDFTIEEIWVGVGDLVQDGQVLLK"
     gene            complement(5700..>7214)
                     /locus_tag="PLY05_04235"
     CDS             complement(5700..>7214)
                     /locus_tag="PLY05_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC02546.1"
                     /translation="AFGQTVERLLKTYDEDEQSELFLKGIAADIAKTHKAAYNMEISE
                     IGLWPLLVKILFDAAKEHSVVIFDQEAWIACVSPNTILPESVEVEWQRTVEKFTTNKF
                     PETPKQMKKHFESLLTNLLAPHGFRCVESDPDSLTFQRDTPFISQVLDFGVDWCNDDL
                     RIGVGFSGTTSGIVAALVSKLSHNNNYLLLSEFIKDDVCDIFYVNLFGPLTHNFKKEI
                     GHSLNIIKSELDVIRVLNLIKNLVLPVLDFIKDLKSAHIALNTDKFPDFRPAATVFRQ
                     YQEAALAIARLANPNNLEEFERVVSDYIKSNLEVTQTKDWEWFVDSLRKLDVEDFFEE
                     QKRLKEEVKLKEQQYQDTIKEHLKKKTPSELMNLGEWYDAETNLIWQRYCLGQAWQNG
                     NISGQGSQITWTKAVELTNAKEIKDFGWRLPTEKELASLMLPDKEGYKTEQGILFCPK
                     EEGQYATFWTSTTEKNSDNANVVVFYEGKVRKYPKGYGCFVRLVRNGKNSILRK"
BASE COUNT         2014 a         1426 c         1443 g         2231 t
ORIGIN      
        1 aaaaaatgtt ggcaaccatt ttgtttaagc acataggcaa attcaaaact ataggtgtaa
       61 tctactttgt gaaaagcggt ttttgtgcct gcctgtttga ttgtcgtccc caaacaacaa
      121 aaagcttcat cggcttttac atcttttagg gcgctggtta atttatcaaa atcaatcact
      181 aaatttttta gtttttcatg agcagcgatg ggtttacggg ttactgcaat cacctgttta
      241 tacacagatg acgctaataa atgtgctaac acctgtttac ctaccatgcc tgtcgcacca
      301 ataataattg cggtttttcc gctcataatc tcgctccagt ccaatcacaa cacattcgct
      361 cgtcgaacga cgcaaacagc actctagttt attgcatttt gctcattatg cctattcatc
      421 aacatttagc aatcatttcc ttgagctttt actctagcca agcaagcatt tgctcggtat
      481 tattcgcata aatttaaaaa cataatgagg aagaccacca tgagctatac aggtatgaat
      541 ttttgtttgg gtgaaaccat gcagcaatta cgcgatacgg tttataaatt tgcccaagaa
      601 gaaattgcac ctcgtgccgc tgacattgat aaaagcaatg agttcccatt ggatttatgg
      661 cgtaaatttg gcgatttagg cttattaggc atgaccgtca gtgaagaata cggtggtgtt
      721 gatatgggat atttggcaca tgtgcttgcc attgaagaaa tttctcgtgc ttctgcatca
      781 gttggcttgt cttatggtgc gcattctaat ttatgtgtca atcaaattta tcgtaatggt
      841 aatgctgaac aacgtgctaa atatttgcct aaattaatca gtggtgaaca tattggtgca
      901 ttggcgatga gtgaaaccac cgctggctct gatgtggtga gcatgaagct tcgcgccgat
      961 aaagttgaag gcggctattt attaaatggc cacaaaatgt ggattaccaa tggccctgat
     1021 gcccatactt atgtgattta tgccaaaacc gatttaagct gccaagcctc taaaggcatg
     1081 accgcgttta ttgtggaacg tgattggcaa ggttttagcc gctcacctaa actcgataaa
     1141 ttaggcatgc gtggttctaa cacctgtgaa ctggtattta atgactgttt tgtgccagaa
     1201 gaaaatgttt taggccatgt gggtggtggt gccaaagtat taatgagtgg cttagattat
     1261 gagcgcatcg ttttagcggc tggccctgtg ggcgtgatgc aagcttgtat ggacattgtt
     1321 attccttata tgcacgaacg caagcagttt ggtcaagcaa ttggtgagtt tcaattaatt
     1381 caaggcaaaa ttgctgatat gtataccaaa cttaatgcct ctcgtgctta tttatatgct
     1441 gttgcacaag cctgtgatcg tggtgaagct gcccgcaaag atgctgctgc aaccatttta
     1501 tattgtgctg aaaatgcaac ccaatgtgct ttggatgcaa tccaaatttt aggtggcaat
     1561 ggttatatca atgactatgc gacaggtcgt ttgttgcgtg atgccaaatt atatgagatt
     1621 ggtgcaggta cttccgaaat tcgccgtatg ttaattggcc gtgaattgtt taacgaaacg
     1681 agataataaa atgcctattt tacatagcca aattaatccg catagtccga gctttttaga
     1741 aaatgctcaa gctttacgcg aacaagtctc tgaactaaaa aaagttttac aagatgtggc
     1801 caaaggtggt ggtgaagccg ccgtggctcg tcataaagcc cgtggcaaac tcactgctcg
     1861 cgaacgtatt aatgccattt tagatgttgg ctcacctttt ctagaattag cccctctcgc
     1921 cgcttaccaa gtttatggta aagaagatgt accagccgca ggtattgtcg ctggcattgg
     1981 ccgcgtgggt ggtattgagt gtgtgattgc cgctaatgat gccacggtta aaggtggctc
     2041 ttattatcct ttaaccgtaa aaaaacacct acgcgcccaa acgattgccc aagacaatca
     2101 cctaccctgt atttatatgg ttgactctgg tggcgcaaac ttgccccgtc aagatgaagt
     2161 ctttccagat gaacgtcact ttggccgtat tttctttaat caggctaata tgtcggcaat
     2221 gaatattcct caaattgcgg tggtgatggg ttcttgtacc gcaggcggtg cttatgtgcc
     2281 atcgatggcc gatgaagcca ttattgttaa agaacaaggc acgatctttt tggcaggccc
     2341 gcccttagtc aaagcggcaa caggcgaaat cgtcagtgct gaagatttag gtggtgctga
     2401 tttacattgt cgtacctctg gtgtcaccga ccaattagcg gataatgatg aacatgccat
     2461 tgccttggcg cgtgctgccg tttctcggtt aaatcgtcca aaaaatattg atttagccat
     2521 tcgtccacct aaaccgccac gttattctgc cgaagaaatt tatggcatta ttcctaaaga
     2581 cccacgtcaa ccttttgatg ttcgtgaaat tattgcccgt ttagttgatg actctgagtt
     2641 tgatgaattt aaacctttgt atggctctac cctcgtttgt ggttttgctc attggtgggg
     2701 ttatcctgtg ggtattgttg ccaataacgg tattttgttt agtgaatcgg cacaaaaagg
     2761 cgcgcatttt attgagcttt gtagccaacg tggtattccc ctgattttct tacaaaacat
     2821 tactggcttt atggttggta aaaagtatga agaaggtggc attgccaaac atggtgcaaa
     2881 aatggtcact gcggtggctt gtgccaaagt gcctaagttt accgtgttaa ttggtggctc
     2941 ttatggtgca ggtaactacg ggatgtgtgg tcgtgcttat agcccacgct ttttgtttag
     3001 ttggccaaat tctcgcattg cggtgatggg tggtgaacaa gcagccagcg tactcaccga
     3061 aattcaaaaa ggtgcaaacg ctaaacgtgg tgtacattgg acagccgaag aagaagccgc
     3121 ctttaaacaa ccttatttag aaaaatatca acgtgaatct catccatttt atgccagtgc
     3181 gcgtttgtgg gacgatggtg tgattgaccc cacgcaaact cgtgatgtct taggcttatg
     3241 tatttcagca agcttaaatg cacccagcca agagacaaaa tttggcgtgt ttaggatgta
     3301 aaccatgtta gaacaccaaa ttgaccaccg aggtgttgtt accctcaccc taaatcgtgc
     3361 cgaactgcat aatgcctttg atgatttatt aattgctgaa ttaattaaaa cactcaaact
     3421 ttatgagcaa aattctaaaa cacgattatt agtcttgcgc tctacaggta aaagcttttc
     3481 cgcaggtgct gacttaaatt ggatgaaacg tgttgcccaa tatagttttg ccgaaaacaa
     3541 acaagatgct gaacaactcg ccgaattaat gtcatgctta tatcattttc caagcccaac
     3601 tattgccgtt gttcaagggg cggcctttgg tgggggtgta ggtttagtca gttgttgtga
     3661 catagccatt gccagtgaca aagctagttt ttgtttatca gaagttaaat taggcttagt
     3721 ccctgcggtg attagtcctt atgtgattgc tgccattggc gcacgtcaag cacaacgcta
     3781 ttttttaacc gctgaacgtt ttgatgccca aactgccttt catttaggct tagtgcaaca
     3841 actcacttca gccgatagcc ttgagcaagc tgctgatagt ttaatcacta ccctcttaca
     3901 aaactctcct caagctctta aagcctgcaa aaaattaatt caggatgttg atggggcaat
     3961 taatccacaa ttgcgcgata tgaccacgac attaattgcc aaattacgtg caagccctga
     4021 aggcaaagaa ggcttaagtg catttttaga aaaacgtcct gctggatggt tannnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntttgttgc cgaagaatta cccgaagcga
     4201 ttcaagccgc gcaacgtgaa ggccaaaata gctttggtga tggtcgttta ttaattgaaa
     4261 aattattggt taaaccgcgt catgttgaag tgcaaatttt ggccgacaaa catggccatt
     4321 gtgtatattt atttgaccgt gattgctcct tacaacgtcg tcatcaaaaa gtcgttgaag
     4381 aagcgcctgc tcccgattta agccctgaac tacgtgcggc aatgggacag gcggctgtaa
     4441 aagcggctca agccattggc tatagtaatg ctggcacgat tgagtttttg gtggccaata
     4501 atcaatttta ttttatggaa atgaataccc gtttgcaggt agaacatccc gtcaccgaaa
     4561 tgattagcgg cctagattta gtcgaatggc aattacggat tgccgcaggt gaacatttaa
     4621 ccttgcagca agaaaaccta aaagccaatg gtcatgccat tgaagtacgt ttatatgccg
     4681 aagaccctgc ccatgacttt ttacctgcga ttggtaagct aaatcaattt agctacaagc
     4741 aacaacaccc ttggcaacgc attgatacag gttttcaaac aggtgatgaa atctcccctt
     4801 tttatgatcc gatgttagct aaggtcattg cttggggtga aaatcgtcaa ttagctattg
     4861 cccgtttatt acaaaccttg gccgatattc gcattagtgg cttacgccat aacctgagtt
     4921 atttacaacg agtcattgct caccctgatt ttgccgatag tcaacattta gccactgact
     4981 ttttggtatt gcgccatcaa accttattaa atcaagacag cccctaccag caagctgctt
     5041 tattagtggc tgccgcttat tattggcaac agcaacaaca aaatatttct gtggttagca
     5101 atgatttata ttcgccttgg caaaatttgg tggcatggcg aacattaggc gacaactata
     5161 gcagcttaca acttgttgtt aacgaacaaa accaacagct acaattgcaa aaacaaggtg
     5221 aaaattttgt ttggcaacag gccgagcaac aaggacaatt tagctttaaa caagataacc
     5281 atgagtttat ttatcaagat gctcagcaac gtttacgttt tcaagtgacg ctggatagtc
     5341 atggtttgca attagcccat gaaggtgtga tttggcaatg tcgtttatta gagccttata
     5401 aacaaagtca acaacagcaa aaccaacata aaggctattg tgcgccaatg actggccgta
     5461 ttaccgcagt tttggtcaat gccgaacaag cagtgaccaa aggtgaaact ttagtagtga
     5521 tggaagcgat gaaaatggaa catcgcattg ttgcccaaga cgattttacc attgaagaaa
     5581 tttgggttgg tgtgggtgat ttggttcagg acggacaagt gttgttgaag tgatttgtat
     5641 ttaaaaatct caatctgtgt gtatctcttt taatgcagga gatacacctg agttatctct
     5701 tattttcgaa gaatgctatt ttttccattt cttactaagc gaacaaaaca accataacct
     5761 tttggatatt tacgaacttt gccttcataa aaaaccacaa catttgcatt gtcactgttt
     5821 ttttcggtag tagatgtcca aaatgtggcg tattgtcctt cttctttagg acaaaataaa
     5881 ataccttgtt ctgttttata accttcttta tctggcaaca ttaaactggc tagttctttt
     5941 tcagtaggta aacgccagcc aaaatcttta atttcttttg cgttagttaa ttcaaccgct
     6001 tttgtccatg ttatttggct gccttgtccg ctgatattac cattttgcca tgcttgacct
     6061 aagcaataac gctgccatat taaatttgtt tctgcatcat accactctcc taaattcatt
     6121 aattctgatg gtgttttttt ctttaaatgc tctttaatgg tgtcttgata ttgctgctct
     6181 tttaatttaa cttcttcttt taaacgtttt tgttcttcaa aaaaatcttc cacatctaat
     6241 tttcttaaac tatcaacaaa ccattcccaa tctttagttt gagtcacttc taaattggat
     6301 ttaatataat cagatactac tcgttcaaat tcttctagat tattggggtt agctagacgt
     6361 gcaattgcta aagctgcttc ctgatattgt ctaaaaacag ttgctgcagg cctaaaatcg
     6421 gggaacttat ctgtgtttaa agcaatatga gcacttttta aatcttttat aaagtccaac
     6481 acaggaagta ccaaattttt aattaaattt agcactctaa tcacatctaa ttcgctctta
     6541 ataatattta aactatgtcc tatctctttt ttaaaattat gagtgagagg gccaaataag
     6601 ttaacataaa atatgtcaca aacatcatct ttaataaatt ctgacaatag caaataattg
     6661 ttattatgtg acagtttact taccagtgct gcaactatgc cgcttgttgt tccactaaaa
     6721 cctacaccaa ttctaagatc gtcattacac caatcaactc caaaatctaa aacttgagag
     6781 ataaatgggg tatctctttg aaaggttaaa ctatcaggat cactttctac acatctaaaa
     6841 ccatgagggg ctaataagtt agttaacaat gactcaaagt gttttttcat ttgttttggc
     6901 gtttcaggaa atttattagt tgtaaatttt tcaacggttc tttgccattc tacctctacc
     6961 gactcaggta aaatcgtatt gggtgacacg cacgcaatcc acgcttcttg gtcaaaaata
     7021 accacggaat gttctttggc agcatcaaat aaaattttta ctaataatgg ccacagtcca
     7081 atctcagaaa tttccatgtt gtaagcagct ttatgcgttt tagcaatatc ggcagctatc
     7141 ccttttaaaa acaattcact ttgttcatct tcatcataag ttttgagtaa tctttctacg
     7201 gtttgaccaa aagc
//
LOCUS       DAOQMB010000076         7203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4285_length_7203_cov_6.684387, whole genome shotgun sequence.
ACCESSION   DAOQMB010000076 DAOQMB010000000
VERSION     DAOQMB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4285_length_7203_cov_6.684387"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..461
                     /locus_tag="PLY05_04240"
     CDS             210..461
                     /locus_tag="PLY05_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005804883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HNC02547.1"
                     /translation="MQAQPQNVPIFKAVHSANLGDRLLRRVEVEHKTGLSRSTIYDGI
                     RKGTFPEPVPLVGKSVAWLCSEIDAWIADKVAKRNQKSA"
     gene            489..755
                     /locus_tag="PLY05_04245"
     CDS             489..755
                     /locus_tag="PLY05_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02548.1"
                     /translation="MGTVTIKANNQTLTLSSDDTVALEKALIQAKKAPNQDIQQMTNS
                     GIMLVTFMPDARANFSRSIKPTDAILSPEQKPFFTAKEVVQTDC"
     gene            869..1210
                     /locus_tag="PLY05_04250"
     CDS             869..1210
                     /locus_tag="PLY05_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02549.1"
                     /translation="MHLLIEAYGKLFSIDFDALEALKTAINKAVGASPRPKVAEPSVV
                     GIIQARSLGETLLIKAANQQISIISQDVPFLQEALNNVGAVLNSPITKETDKGDIGVM
                     CYEPLEEVLNE"
     gene            1306..2013
                     /locus_tag="PLY05_04255"
     CDS             1306..2013
                     /locus_tag="PLY05_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02550.1"
                     /translation="MCMPVNQFLGSLVQAVPTFTRQCDMFRNQGRTATVGGNGSPRQA
                     QYILFTPLTTTTDTANNDGTGLHCQLGLVAQYNWRTTAQAVFLCVPYGSIVLMGCMGR
                     RSRLPVPVTGRPTLYSPSPNAWSLLVMDLIPITGASTMTTPTQKPSSVSQTFAAVICI
                     LQNLNPDTYTHTSTLLAAFKAQGIKRDLRSIQRYLVALADYSLVEHDNHHPKGWKLRT
                     NPNKYAIEAILAMGVQA"
     gene            2010..2456
                     /locus_tag="PLY05_04260"
     CDS             2010..2456
                     /locus_tag="PLY05_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02551.1"
                     /translation="MSPRQPTARKSTKNYSPAMRQYLAQLTDLAWQAEHLEGEYWQVV
                     TAKGETLAIVNDTVTQPNINAQQAAQLMADAPLLLKELLLAHHLLRDTVTRLFTNEKK
                     VTFEDIKPTSERYDQRLRLLAKYRDLIENREADYAPLLANKEQGHE"
     gene            2449..2691
                     /locus_tag="PLY05_04265"
     CDS             2449..2691
                     /locus_tag="PLY05_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02552.1"
                     /translation="MNKTDFICTEEQMRKLDVLDAHLHVLSALSSAITRNDYPLDLSA
                     DKLATLLYTLSNDLTEALSNIKLHTHHQKKEHSHDD"
     gene            2681..3688
                     /locus_tag="PLY05_04270"
     CDS             2681..3688
                     /locus_tag="PLY05_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNC02553.1"
                     /translation="MTTNSTLINSIRQVAACALNHSAMLVPEWLPDGKRHKNDWIAKN
                     PVRGDRHAGSFSVSLISGKWHDFADNTAKGGDLVGLYAYLKGCPQLQAAQAIDSRLGL
                     GIFTQSGQAYKVDKAKQEANLQVAQERKQKAQAMEEYDRQAAKEQAKWLWHNAKLANH
                     QHPYLVKKFVFAHGLRQNKQGALLVPICCQGELVNLQQINVEGEKRFLAGGQVKGCYS
                     PIGRIDTRKPLYICEGWATGATLHQQTTSAIACALNAGNLKEVALALRARYGDSLEII
                     IAGDDDRKNPNNTGRKAANDAALAIGALAIFPEWPSHAPIELSDFNDLALWQMQQGAS
                     A"
     gene            3685..5436
                     /locus_tag="PLY05_04275"
     CDS             3685..5436
                     /locus_tag="PLY05_04275"
                     /inference="COORDINATES: protein motif:HMM:NF017826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HNC02554.1"
                     /translation="MNQLMQPTTDSKTQVQLEPQKRPYYALYQQEVTVDGQSYKSGVW
                     LHGIKHNNHQDSKGVPFDEWLCVPLSVEAETISTDDGKVGRLLRFYHHGKLNEWVMPM
                     EAIAGSGEEVLKVLMDKGLAVNFPKRKTIINYIAMQQMHTIIETTYKTGWHKSGAFVL
                     PNQVLGGDKVRYQDSGKAATIFSSQGTLEAWKKSVSLLCVGNPVLILSVCCALAGTLL
                     FKVSIKGGGVHLVGDSSSGKSLAQEVAASVWGHPDSFAASWDVTKGGIEIEAASRNDT
                     VLILDEIKRADPYKVQEMAYAIANGIGKSTMTREREGRAKLTWRVLALSSGERSLSEH
                     AAIAGSTAHAGAELRMVDVNAGTRTYKAFDYLHGLADGQQFHSQLTKSCRQHYGHAGA
                     AMVEQLLKQKDDSFYLNAFTDMRKGFDAQNPQAGRVADRFAVMALAGELAIRYGVLSW
                     PVGTAKNACQLLFNEWLATVGHGNTEDKQIAQALVDFIDKYGDSRFSNIDDSFPSVIH
                     NRAGYYRLEDGKKLYLFNNAALKEAAQGYSLKRIIQTLEVTGALAKRDTDRPQKRFRF
                     PFGGDERFIVIDPEKLE"
     gene            5883..6632
                     /locus_tag="PLY05_04280"
     CDS             5883..6632
                     /locus_tag="PLY05_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02555.1"
                     /translation="MNDTISHLPENIQDAINNHEAAKQFFLEKKSAYQAVLAKVEKHR
                     KTAQAARSEAEQAGQTWRELLRQSDGALTKEVNALRQKETDSHALAEEFDKIAVEAEA
                     IVKQHGQAAYDAFVAHNKAHSLAISLYDEMTAQTAIAELLALPQAKTLAWHLQHIKQQ
                     NLNKVVDMFGGAVTNQDQEDYLNTATQKTASFLLDLFEGLIKDLPVSDGYDEVRKTLI
                     LEPLSPINGSQFKPKTPTQLHLERFKSQQAG"
BASE COUNT         2214 a         1431 c         1611 g         1947 t
ORIGIN      
        1 tgacaagctt acagcaattc ttaaattctt taacctgtca atgcgtaagt cctattgtat
       61 atgccatata caactttttt cagatagaaa ttatttttgc ctcatgcaca atagctttac
      121 cgagtgaagc taggtgaatc catacggaac tttaaggatt ctatctagca agctttaacc
      181 gtaaatgtgt gcagtgaagg ggttcgataa tgcaagcaca accgcaaaac gtaccaattt
      241 ttaaagcggt acatagcgca aatttaggcg accgtttgtt aagacgtgtc gaagtagagc
      301 ataaaacagg tttaagccgt tctactattt atgatggtat ccgaaaaggt acttttcctg
      361 agcctgtacc gttggtgggt aaatctgttg cgtggttatg tagtgaaatt gatgcgtgga
      421 ttgctgataa ggttgctaaa cgcaatcaaa aatcagcgta attttaaatt tttaagtgag
      481 taaaaaacat gggtactgtg actattaaag ccaataacca aactttaacg ctatcgagtg
      541 atgatactgt cgcgcttgaa aaggcattga ttcaggccaa aaaagcccct aatcaagaca
      601 ttcaacaaat gactaatagc ggcattatgt tggtaacctt tatgcccgat gcacgcgcta
      661 actttagccg ttctatcaag cctacagatg ctattttgtc acccgaacaa aagccttttt
      721 ttacagctaa agaagtcgtt caaacagact gctaattcca tttattaagt cccattaatc
      781 gcgcaaaaac aaagcattaa ctaatgctat ggaggagttt tgcgcctaaa aaatgggtta
      841 attattttta aatcaagagg tcaacattat gcacttactg attgaagcct acggaaaact
      901 attttcgatt gattttgacg cgcttgaagc ccttaaaacg gccattaata aggcggttgg
      961 tgcttcaccg cgcccaaagg ttgccgagcc aagtgttgtc ggtattattc aagcacgttc
     1021 tttaggggaa accttgttaa ttaaggcggc taatcaacaa ataagtatta tttcgcaaga
     1081 tgtgcctttt ttacaagaag ccttaaacaa cgtgggggca gttttgaata gtcctattac
     1141 aaaagagaca gacaaaggcg acataggcgt gatgtgttat gaaccgcttg aagaggtttt
     1201 aaacgagtaa acgcataggc attaaaaagc ccgaataacg cgctaacact atcgggctga
     1261 ttaaattaga tactttttaa taactcgcta agggagcata acacaatgtg tatgccagtc
     1321 aatcagtttt taggcagctt ggtgcaagct gttccaacct ttacacgcca atgcgatatg
     1381 tttaggaatc aaggccgcac cgctactgta ggcggtaatg gttcgcctag acaagcccaa
     1441 tatattttat ttaccccctt gacgacaacc acagacacgg ccaataatga cggcactggc
     1501 ctacattgcc agttgggctt ggtcgcccaa tataactggc gcacaaccgc gcaagcggtt
     1561 tttttgtgcg tgccgtatgg ctctatcgtt ttgatgggct gtatggggag gcgttcgcgc
     1621 ctgccagttc cagttactgg tcgaccaacc ctgtacagtc cgtcacctaa tgcttggtcg
     1681 cttttggtga tggatttaat tcccataact ggagcgtcca ccatgacaac ccccacccaa
     1741 aaaccgtcta gcgttagcca aacgtttgcc gctgttattt gcattttaca aaatcttaat
     1801 cctgatacct atacccacac aagcacacta ttagcggctt ttaaagcaca aggtattaag
     1861 cgtgatttac gttctattca acgttattta gtggccttag ccgattactc gctagtcgag
     1921 catgataacc atcatcctaa aggctggaag ctacgcacca accccaacaa atacgcgata
     1981 gaagcgattt tagcaatggg ggtgcaagca tgagtccacg tcaacccacc gcgcgtaaaa
     2041 gcacaaaaaa ttattcgcct gccatgcgtc aatacttagc gcaattaacc gatttagcat
     2101 ggcaagccga gcatttagag ggcgagtatt ggcaagtcgt caccgccaaa ggcgaaacct
     2161 tggcgatagt taacgacact gtgacacaac caaacattaa cgcacaacaa gcggcacagt
     2221 taatggccga tgcccctttg ctactcaaag agttgttatt agctcaccac ttattgcgtg
     2281 ataccgtgac gcgtttattt accaatgaga aaaaagtcac ttttgaggat attaaaccga
     2341 ctagcgagcg atacgaccaa cgtttgagac tactcgccaa ataccgtgac ttgatagaaa
     2401 acagagaggc cgactatgcc cctttgttag ccaacaagga gcaaggccat gaataagacc
     2461 gattttattt gtaccgaaga acaaatgcgt aaactagatg tgttagacgc gcatttacac
     2521 gttttaagtg cgttatcgag tgcaataaca cgcaatgatt accccctaga tttaagtgcc
     2581 gacaaattgg caactctgct ttataccttg agcaatgact taacagaggc tttaagtaat
     2641 atcaagctgc atacccatca tcaaaaaaag gagcatagcc atgacgacta atagcaccct
     2701 aattaatagt attcgtcaag tggctgcttg tgcgcttaac cattctgcta tgttagtgcc
     2761 tgaatggttg cccgatggta aacgccacaa aaacgactgg attgctaaaa accctgtccg
     2821 tggcgatcgt catgcaggtt cttttagcgt gtctttaata tcgggtaaat ggcatgattt
     2881 tgccgataac accgccaaag ggggcgactt agtgggctta tatgcctact taaaaggttg
     2941 cccacaatta caggccgcac aagcgattga tagccgttta ggtttaggga tatttaccca
     3001 atcaggtcaa gcctataagg ttgataaagc taaacaagaa gcgaatttac aagtggctca
     3061 agagcgtaaa caaaaggctc aagcaatgga agagtacgac cgccaagccg ccaaagaaca
     3121 agccaaatgg ctatggcaca atgctaagtt agccaatcat caacacccct atttagtgaa
     3181 aaagtttgtc tttgcacatg gtttacgcca aaacaaacaa ggggctttat tagtgcctat
     3241 ttgctgtcaa ggagagttag tcaacttgca gcaaattaac gtagagggcg aaaaacgctt
     3301 tttggcagga ggacaggtaa aaggctgtta ttcgccaata ggccgtattg atacacgcaa
     3361 accgctttat atttgtgagg ggtgggcaac aggtgcaacc ttgcaccaac aaaccactag
     3421 cgcgattgct tgcgccttaa atgcaggcaa tttaaaagag gttgccttag ctttgagagc
     3481 gcgttatggc gatagtttag aaattattat tgcaggtgat gacgaccgca aaaaccctaa
     3541 taacacaggc agaaaagcgg ccaatgatgc cgccttagcc ataggcgcat tagccatatt
     3601 tcctgaatgg ccaagccatg cacctattga gttaagcgat tttaatgacc ttgctttgtg
     3661 gcaaatgcaa caaggagcaa gtgcatgaat caattaatgc aaccaacaac cgactcaaag
     3721 actcaagtgc aattagagcc tcaaaagcgg ccatattatg ccttgtatca acaagaggta
     3781 actgtagatg ggcagtctta taaatcgggt gtatggctac acggtattaa acacaataat
     3841 caccaagaca gtaaaggtgt tccctttgat gaatggctat gtgtgccttt atcggttgaa
     3901 gccgaaacca taagcaccga tgacggtaaa gtagggcggt tattgcgctt ttatcatcat
     3961 ggtaaattaa atgaatgggt catgccaatg gaggcgattg caggcagcgg cgaagaggtg
     4021 ttaaaggtct tgatggacaa aggtttagcg gttaattttc ctaaaagaaa gaccattatt
     4081 aactatattg ctatgcagca aatgcacacg attatagaaa ccacctataa aacaggctgg
     4141 cacaaatcag gtgcatttgt attacctaat caagtgctag gtggtgataa ggttcgttat
     4201 caagattcgg gcaaggccgc gactattttt tctagtcaag gcacattaga ggcatggaaa
     4261 aagtctgttt ctttattgtg cgtgggcaat cctgttttaa ttttgtcagt ttgttgcgct
     4321 ttagcaggca cattgttgtt taaggtgagt atcaagggcg gtggtgtgca tctagttgga
     4381 gattcctcta gcggtaaatc tttagcccaa gaggttgccg cctcggtatg gggacaccct
     4441 gatagttttg ccgcttcatg ggacgtcacc aaaggcggta tcgaaattga ggccgcttca
     4501 cgtaatgaca cggtacttat tttagatgag attaaacgag ccgaccccta caaagtgcaa
     4561 gaaatggcct atgcgattgc taacggcata ggcaaaagca ccatgacccg tgagcgagaa
     4621 ggccgcgcca aattaacatg gcgtgtgctt gccttatcga gcggcgagcg ttcgctatct
     4681 gaacacgcgg ccattgctgg cagcactgcc catgcagggg cagagctacg catggtggac
     4741 gttaatgcag gcactcgcac ctataaggcc tttgattatc tacacggttt agccgacggt
     4801 caacaattcc atagtcaatt aacaaaatca tgccgtcaac attatggcca tgcaggggcg
     4861 gcaatggtcg aacagttgtt aaaacaaaaa gacgatagtt tttatctaaa tgcgtttacc
     4921 gatatgcgaa aagggtttga tgcacaaaac ccacaggcag gccgtgtggc cgaccgtttt
     4981 gcggtcatgg ccttagctgg tgagttagca atccgttatg gcgtgttatc gtggcctgtt
     5041 ggtacggcaa aaaatgcttg tcaattactg tttaatgaat ggctggcaac cgttggccat
     5101 ggtaataccg aagataagca aatagcccaa gccttagtcg attttattga taaatatggt
     5161 gatagtcgct tttctaatat tgacgatagt tttcctagcg ttatccacaa tcgagcaggt
     5221 tattaccgtc ttgaggatgg caaaaaactc tatcttttta ataatgcggc actcaaagaa
     5281 gcggcacaag ggtatagctt aaaacgcatc attcaaaccc tagaagtgac tggcgcatta
     5341 gcaaaaagag ataccgatag accgcaaaaa agatttcgtt ttccttttgg tggggatgag
     5401 cgttttatcg tgattgaccc cgaaaaactg gagtaaaaat ctagttttat tttgtcattt
     5461 aagccaatag atatattttt ttagtaggcg gctatcaaaa acgataatca tgcctacagg
     5521 ggatttttgt gccaaaaaaa agcattttta gattttgtcc catttttttc ttgggaacaa
     5581 atgaattttt gggaactttt taaaaaaatt tgggaacaga cgacctagta ttatcaaggc
     5641 ttagaggcac ttgttcccat gttcccaaga atcccaagcc aaaactagag agagagaaag
     5701 agggggggta cacacacagt aaaattagag agttattttt gtcatttagg tcaattagtg
     5761 tttttatttt tagagtaaat ggaggtgtca aaaaagccaa taaagcaaat gtaacagcct
     5821 atatgggtag gctaaaaact agacgattta aattttttta acgcaacaat aggtattcaa
     5881 ttatgaacga tacaattagc catttacctg aaaatataca ggacgctatc aacaaccatg
     5941 aagccgctaa gcaatttttt ttagagaaaa aatctgccta tcaagcggtg cttgccaaag
     6001 tcgaaaaaca ccgtaaaaca gcacaagccg cgcgtagcga agcagagcaa gcaggccaaa
     6061 catggcgcga attgttacgt caaagtgatg gcgcattgac caaagaagta aatgctttgc
     6121 gtcaaaagga aacggatagt catgccttag cagaggagtt tgataagatt gctgtagagg
     6181 cggaggcgat tgttaaacaa catggccaag ccgcttatga tgcctttgtg gcgcataaca
     6241 aggcgcatag cttagcaatt agcttgtatg acgaaatgac agcacaaaca gccatagcgg
     6301 agttattagc cttgccacag gcaaaaacct tagcgtggca cttacaacat atcaaacaac
     6361 aaaatttgaa taaggttgtt gatatgtttg gaggagctgt aacgaatcaa gaccaagaag
     6421 attacttaaa taccgcaaca caaaaaacag caagcttttt gcttgacctg tttgaggggt
     6481 taattaaaga cttgcctgtc agtgatggct atgatgaggt gcgtaagacg ttaattttag
     6541 aaccattatc gcctatcaat ggcagtcaat ttaagcctaa aacacccaca caattgcatc
     6601 ttgaacgctt caagagtcaa caagcaggtt aatcctagta acaacctttt aaaccgcctt
     6661 agatggcggt tttgtttatg taatggcgtt ttttaaaaga attttatttt taataatttt
     6721 tcttgagaaa aagcagttgt gaccccgcca cgcctgccca ctaaatgtgt cgattttgtt
     6781 gcaaatgatg caattagcct aagccttata gctgtaagag ggggtatggc tttatgtagt
     6841 ggttaagctg atgcaaattg acgcaaaatg atgcgttttg atgcaggcta aagagaggag
     6901 gtttttagaa aaaatgctat ttttaaggat tttagacaaa atagtaaaat agggtagtcc
     6961 gagaaaggct tgataaggct taagagggct gtattatatt tggggctgtt ttgttaaaat
     7021 ggttttactc aaggtgatat ttgtataatg gtagagaaaa aggtagagag ttatatcttt
     7081 tattgaaata aatctatatt tattagtggc ttaggtattg ggttgtattc ccgtccgctg
     7141 ccactttatc aagaagctgt aagattaaag tcttacggct tttttgtttt taggggttaa
     7201 aaa
//
LOCUS       DAOQMB010000077         7139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4346_length_7139_cov_17.657397, whole genome shotgun sequence.
ACCESSION   DAOQMB010000077 DAOQMB010000000
VERSION     DAOQMB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7139
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_7139_cov_17.657397"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..379)
                     /locus_tag="PLY05_04285"
     CDS             complement(2..379)
                     /locus_tag="PLY05_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02556.1"
                     /translation="MKYFSVLLVASLLTACANTPSSQVTGTLPSHTIMKEGGLYLTSG
                     YGISKDESEKAATFNANQSCAVFKKLPVVNKMDSQYQGTVSEDVNKTVNMVSGIVKSM
                     TGKNYGTTATDKDYKTSLEFRCQ"
     gene            complement(389..706)
                     /gene="yhbY"
                     /locus_tag="PLY05_04290"
     CDS             complement(389..706)
                     /gene="yhbY"
                     /locus_tag="PLY05_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002115576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HNC02557.1"
                     /translation="MSLSIAEKKRFRQIGHQLNPVVLLGGHGLTEAVLAEIDRALEDH
                     ELIKVRLGGEDRELRLAMIDEIVQSTNCQAVQTIGKLVLLYRAAKKPNPRLSNILRAN
                     NAL"
     gene            812..1453
                     /gene="rlmE"
                     /locus_tag="PLY05_04295"
     CDS             812..1453
                     /gene="rlmE"
                     /locus_tag="PLY05_04295"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004801593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HNC02558.1"
                     /translation="MSVRIHNNKLSKSSKDWMREHLEDSYVQKAQKDGYRSRAAYKLL
                     EVNQKDKLFKAGMTVVDLGSAPGSWSQIAAQLVGTNGMVLASDILPMDALPNVHFIQG
                     DFREDDVFNKLLDTLNGRPVDLVISDMAPNIGGNASDQPRAMYLCELALDFALRVLKP
                     NGQFLVKVFQGEGYEEYRKTIMDNFTVLKSRKPDASRARSREVYLLATGFKVK"
     gene            1566..3389
                     /gene="ftsH"
                     /locus_tag="PLY05_04300"
                     /pseudo
     CDS             1566..3389
                     /gene="ftsH"
                     /locus_tag="PLY05_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
     gene            3465..4304
                     /gene="folP"
                     /locus_tag="PLY05_04305"
     CDS             3465..4304
                     /gene="folP"
                     /locus_tag="PLY05_04305"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNC02559.1"
                     /translation="MMKQKLICGDKVLDLSSPVVMGVLNVTPDSFSDGGKFFTLNDAL
                     RQAELLISEGAAILDIGAESTRPQAQLVTEQQELDRLLPIIEAVKQRFDTIISVDTSS
                     PQVMLQAAKLGIGLINDVRALQRDGALQAAAQTKLPICLMHMQGEPSTMQQSPYYQSV
                     IDEVMVFLQQRIEYCLDAGIPAEQLLLDVGFGFGKTLAHNIELLAKLDIFTQLPYPLL
                     VGMSRKSMLGKILNDAPVDQRLFAGLSAATIAALKGAKIIRTHDVKATKDALAVVSAI
                     QHY"
     gene            4317..5654
                     /gene="glmM"
                     /locus_tag="PLY05_04310"
     CDS             4317..5654
                     /gene="glmM"
                     /locus_tag="PLY05_04310"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004639966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNC02560.1"
                     /translation="MARKYFGTDGIRGLVGEFPIVPDFALKLGWAAGKVLAASGNASV
                     VIGKDTRLSGYVLESALQSGLCAAGVDVRLVGPMPTPAIAHLTRAFHASAGVVISASH
                     NPYYDNGIKFFGSNGKKLSDDIEAEIEAWLDKPLAIDNFDDLGKSLRQEDARGRYIEF
                     CKATFPYHLSLKGLKIVVDCANGATYQVGPAVFRELGAEVIAVACEPDGTNINADCGS
                     THPQTLQAAVLVNKADLGIAFDGDGDRVMMVDHTGSVVDGDEIIVMIAKARQQDGFNI
                     SGVVGTLMSNMGMEVAIKEMGLEFIRAKVGDRYVMSALAKRGWTLGGEASGHIVCLDK
                     TTTGDAIIAALQVLSALVQSGQTLHELRQGMSKYPQQLINVKVAKKSDPMENAQVVEA
                     VKDAEAILGARGRVLLRASGTEPVIRVMVEGEQQQMISRICQELADKVRAALG"
     gene            5673..6440
                     /gene="tpiA"
                     /locus_tag="PLY05_04315"
     CDS             5673..6440
                     /gene="tpiA"
                     /locus_tag="PLY05_04315"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNC02561.1"
                     /translation="MYRKKIVAGNWKMNGSVTKNVQLLSALTQSAPDNIEIILAPPAL
                     YLSGVKAQLMGSSIALAGQNVAAEGQAGAYTGEISAQMFADIGVSYAIVGHSERRQYY
                     AETDSLVAKKCLRLREQGLKPIICVGESLAERESGQAEAVVSRQLKAVIDYCGVELLA
                     DAILAYEPVWAIGTGKTATPHDAQAIHAFLRQLVTIYDKNIANYLPILYGGSVKADNA
                     ASLFSMPDIDGALVGGASLVAQDFLAICHAAKASMVG"
     gene            6445..6687
                     /gene="secG"
                     /locus_tag="PLY05_04320"
                     /pseudo
     CDS             6445..6687
                     /gene="secG"
                     /locus_tag="PLY05_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456770.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
     gene            6880..6964
                     /locus_tag="PLY05_04325"
     tRNA            6880..6964
                     /locus_tag="PLY05_04325"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6914..6916,aa:Leu,seq:gag)
BASE COUNT         2020 a         1253 c         1693 g         2173 t
ORIGIN      
        1 tttattgaca tctaaactct aagctagttt tatagtcttt atctgttgcg gttgtaccgt
       61 agtttttgcc agtcatagat ttaacaatac cactaaccat attcacggtt ttattgacat
      121 cttcgctgac agtaccttga tactggctat ccattttgtt aaccacaggc aattttttaa
      181 agacagcaca actttgattg gcattaaagg ttgctgcttt ttcgctttcg tctttagaga
      241 taccataacc agacgttaaa tataaaccac cttccttcat gatggtgtga ctgggtaaag
      301 tacctgttac ttggcttgag ggtgtgttag cacaagcagt taataaactg gcaacgagca
      361 aaacagaaaa atacttcatg taaaaatcct ataaagcgtt attagcacgt aaaatattag
      421 ataatcgtgg attgggcttt ttagcagcac gatataacaa cactaattta ccaatagttt
      481 gcacagcttg acagtttgtg ctttgtacaa tttcgtcaat catcgctagg cgtaattctc
      541 tgtcttcacc acccaaacga actttgatta attcgtgatc ttctaaggcg cgatcaattt
      601 cagccaacac ggcttcagtc aggccatgac cgcctaataa cacgactgga ttgagttgat
      661 ggccaatttg acgaaagcgt tttttttcag caatagataa agacatgaga cataagccta
      721 aaaatttgat taaattctct atacagaatt acctttggcg attggccaaa cgataatact
      781 gactatttta acgtaacttt aggtttaaag catgagtgtt cgtatccaca acaacaaatt
      841 gagtaaaagt agtaaagatt ggatgcgtga gcatcttgaa gatagctatg tgcaaaaagc
      901 gcaaaaagac ggctatcgtt cgcgcgctgc ttataagttg ttggaggtta atcaaaaaga
      961 caaattgttt aaagctggta tgactgtcgt ggatttaggt tcagcaccgg gaagctggtc
     1021 acaaatagcg gctcaattag ttggcacaaa tggcatggtt ttagcttctg atattttacc
     1081 gatggatgct ttgcccaatg tgcattttat tcaaggtgat tttagagaag atgatgtatt
     1141 taataaatta ttagatacgc tcaatggtcg ccctgttgac cttgtaattt ccgatatggc
     1201 acccaatata ggaggtaatg cctctgatca gcctcgtgcc atgtatttat gtgagttagc
     1261 attagacttt gcgcttcgtg tattaaagcc caatggccaa tttttggtaa aagtatttca
     1321 gggtgaaggc tacgaagagt atcgtaagac aattatggac aattttactg tcttaaaaag
     1381 tcgcaagcct gatgcttctc gtgcgcgttc acgagaagtg tacttacttg caacaggttt
     1441 taaggttaaa tagagattta atatacataa gttcttaaaa taatacttat gtaagcttga
     1501 atgttaatca ctgcttggta aataatcggc aagcgcgtaa tgccaataac tgtaggattt
     1561 gacccttgaa cgatatgatg aaaaatttgc ttctatggct agttattgct ggcatgttgg
     1621 tgtttgtatt tagtaacttt aatcaacaag cacccaccaa gcaattaact tattctgagt
     1681 ttgtcaattc tgtcagcagt ggcgatgtga agcgcgtgcg tatagatggt ttaaccatca
     1741 gcggtgaacg tgctaatggt gataaatttg aaaccattcg cccaccgatt cctgaccccg
     1801 aattaatgaa tgacttatta gataaaaaag tcattgttga gggtagtgca cctgaacagc
     1861 aaagtgtttg gatgcagctt ttagtagcaa gcttccccgt tattttaatt attttgttgt
     1921 ttatgttctt tatgcgccaa atgcaaggtg gcggtggggc aggtggacgt ggtgggccaa
     1981 tgacttttgg taaatcacgt gcacgtatgt tatctgaaga tcaaatcaaa accacctttg
     2041 ctgatgtggc aggttgtgat gaagccaaag aagatgtgca ggaagtggtt gagtttttgc
     2101 gtgatcctgc aaaattccaa cgtttaggtg gcaaaattcc ccgtggtatg ctgatggttg
     2161 gtccaccagg tacaggcaaa actttattag cgaaagcgat tgcaggcgaa gcaaaagttc
     2221 cattcttcac tatttctggc tctgattttg ttgaaatgtt tgtgggtgtg ggtgcatctc
     2281 gtgtgcgcga catgtttgaa caagctaaaa aacaagcccc ctgtatcatc tttattgatg
     2341 agattgacgc cgttggccgt caacgtggtg caggtttggg gggtggccat gatgagcgtg
     2401 agcagacctt aaaccaatta ttagtcgaaa tggatggttt tgaaggtaat gaaggcatca
     2461 ttattattgc tgcgactaac cgtccagatg tattagatgc ggcattatta agaccaggcc
     2521 gttttgaccg tcagattgtt gttggtttac ccgatattcg tggtcgtgaa caaatcctca
     2581 aagttcatat gcgtaaagtg ccattgggtg aaaatgttaa tgccgaatta attgcgcgtg
     2641 gtacaccggg tttctcaggt gctgatttag ccaatctagt gaatgaagcg gccttatttg
     2701 ccgcacgcat caacaaaaaa acagttgata tgcaagagtt tgaattagct aaagataaaa
     2761 ttatgatggg tgcagagcgc aaatccatgg tgatgtctga ggctgagaaa cgcaataccg
     2821 cttaccatga agcaggtcat gctattgttg gcagactgtt gccagaacat gacccagttt
     2881 ataaagtatc gattattcct cgtggacgtg ctttgggtgt taccatgttt ttgcctgaag
     2941 aagatcgtta ttcatggtca aaacgtggta ttgaaggtaa aatttgttcg ttatttggtg
     3001 gacggattgc cgaagaaatg accttaggtt ttgatggtgt gacgactggt gcttctaatg
     3061 atattcaacg tgccacagaa ttggcgcgta atatggtcac taaatggggc ttatcacaaa
     3121 aactaggccc aatgttatat gatgaagaag agggcgaagt tttcttaggt cggagtgtta
     3181 cccaacgtaa aaacgtttct gcacaaaccg ccatggatat agacaatgaa attcgtgcag
     3241 tgattgaccg ttgttatagc caagctaaag acattttaga aactaatcgt catattttgg
     3301 aagcaatggc tgatgcttta atgcaatatg aaaccattga cgctgggcaa attgacgata
     3361 ttatggcagg taaaccacct cgtccaccgc atacaggaaa ctcaacatta actgagaaaa
     3421 aagtagaaat cactaaacca gacgattcta atacagccag ttaaatgatg aagcaaaaac
     3481 ttatttgtgg cgataaagtc ctagatttat cgtcacctgt ggttatgggt gtgcttaatg
     3541 tcacacccga ttctttttcg gatgggggaa agttttttac cctaaatgat gctttacgcc
     3601 aagcggaact attaataagt gaaggggcag ctattttaga cattggcgcc gagtccacac
     3661 gcccacaagc acaattagtc accgaacaac aagaattaga tcgtttattg ccaattattg
     3721 aggcggttaa acagcgtttt gataccatta tttcggtcga tactagcagt cctcaagtga
     3781 tgttgcaggc ggctaaatta ggtattgggt taattaatga tgtgcgagct ttacagcgtg
     3841 atggagcatt acaagctgcg gctcaaacca aattgccgat ttgtttaatg cacatgcaag
     3901 gtgagccaag caccatgcag caaagtccat attatcaatc agtcattgat gaagtgatgg
     3961 tgtttttgca acaacgtatt gaatattgtt tagatgcagg aattcctgca gagcaattat
     4021 tgttagatgt tggttttggt tttggtaaga ccttggctca taatatagag ttattggcta
     4081 aactagatat atttacgcaa ttaccttatc cattattagt aggtatgtcg cgtaaaagta
     4141 tgttgggcaa gattttaaat gatgcccctg ttgatcaacg gctgtttgcg ggtctatcag
     4201 cagcgacaat tgcagcgtta aaaggggcaa aaattattcg tactcatgat gttaaagcca
     4261 ccaaagatgc tttagctgta gtgagtgcta ttcaacatta ttaggagtta tcagagatgg
     4321 cacgtaagta ttttggcact gatggtattc gcggtttggt tggtgagttt ccaatcgtgc
     4381 cagactttgc ccttaaatta gggtgggcag caggaaaagt gttggcagca agtggtaatg
     4441 cttcggttgt tattggcaaa gatacccgtt tgtctggtta tgtgttggag tcggctttgc
     4501 aatctggttt atgtgctgct ggtgttgatg ttcgattagt aggcccaatg ccaacaccag
     4561 caattgccca tttaactcgt gcttttcatg ctagtgcagg agtggttatt tcagcttcgc
     4621 ataatcctta ttatgataat ggcattaagt tttttggttc taatggtaaa aaattgtcag
     4681 atgatattga ggcggaaatt gaagcttggt tagataagcc tttagcgatt gataattttg
     4741 atgatttagg aaaaagtttg cgtcaagaag atgcgcgtgg tcgttatatt gaattttgta
     4801 aggcgacttt tccttatcat ttgagcttaa aggggctgaa aattgttgtc gattgtgcta
     4861 atggtgccac ttatcaagta gggccagctg tatttagaga attaggtgca gaagttattg
     4921 ctgttgcttg tgagcctgat ggtactaata ttaatgctga ttgtggctca actcatcctc
     4981 aaaccttgca agcggcagta ttggtgaata aagctgattt gggcattgct tttgatggtg
     5041 atggtgatcg tgtgatgatg gttgaccata caggatcagt ggttgatggc gatgaaataa
     5101 ttgtcatgat cgctaaggct cgtcagcaag atggttttaa tattagtggg gttgttggca
     5161 cgttaatgag taatatgggc atggaagttg cgattaaaga aatggggtta gagtttattc
     5221 gtgctaaggt tggtgatcgt tatgttatgt ctgctttggc caaacgtggt tggacattag
     5281 gtggtgaagc ctctggacat attgtgtgtt tagacaaaac taccacaggt gatgcgatta
     5341 ttgcggcttt acaagtatta agtgctttgg tgcaaagtgg ccaaacttta catgaattac
     5401 gtcaaggaat gagcaaatat cctcagcaat taattaatgt taaggtcgcc aaaaaatcag
     5461 accctatgga aaatgctcaa gttgttgaag ccgttaagga tgctgaagcg atcttaggcg
     5521 cacgaggtcg agtattatta cgtgcttctg gtacagaacc cgttattcgt gtcatggttg
     5581 agggtgagca acaacaaatg attagtcgta tttgccaaga gttagcggat aaagtacgcg
     5641 cagcattggg ttagttattt aggataattt ttatgtatcg taaaaaaata gtggcaggca
     5701 attggaaaat gaatggtagt gttaccaaga atgttcaatt gcttagcgcg ttaacacagt
     5761 ccgcacctga taatatcgag attatcttag cacctcctgc attatatctt tcaggagtta
     5821 aagctcaatt aatgggatca tctattgctt tagcgggaca aaatgtggcc gcagaaggtc
     5881 aagcgggtgc atatactggt gaaatttcag cgcaaatgtt tgcagatata ggggtttctt
     5941 atgccattgt gggacactct gaacgccgcc aatattatgc cgaaacagat agtttagtcg
     6001 ctaaaaaatg tttacgttta agagagcaag gattaaaacc gattatttgt gtgggcgaaa
     6061 gtttggcgga gagagaaagt ggtcaagcag aagccgttgt atctcgccaa ctcaaagcgg
     6121 tgattgatta ttgtggggtt gaactcttag ctgatgctat tcttgcctat gaaccagttt
     6181 gggcaattgg cacaggaaaa acagcaaccc cccatgatgc acaagctatt catgcttttt
     6241 tacgccaatt agtgacaatt tacgataaaa acattgccaa ttatctgcca atcctttacg
     6301 gcggcagtgt aaaagcagat aatgccgcct ctttattttc gatgcctgat attgatgggg
     6361 cattagtggg tggcgcatcg ttggtcgcgc aagattttct cgcaatttgc cacgcggcaa
     6421 aagcatctat ggtaggttaa gtgcatggaa tcctttgttt tagttgtaca tgtcattatt
     6481 gccagtgtca tgattatatt tattttgatg cagcaaggaa agggcgcaga agcaggggct
     6541 tcatttggtg gtggtggttc acaaaccgtt tttggtagtg cgggttctgc tagcttttta
     6601 acaaaactaa ccgctggttt agcgatagtg ttttttatta ctagtatggc attagctgta
     6661 tttgctaaac aaaaagtatc tcaagctccc gtgatttctg caccagttac acaaaaagca
     6721 ccagccaata cagatgtgcc agcaccttct gctaaaacac caacacctat ttctcctgca
     6781 cctgcaaata gtgatgtgcc agcccctgcg cctgcacaaa atactgcacc gtaagactag
     6841 catttactga ataaatacag taaagtagca gcccctgatg cggtggtggt ggaattggta
     6901 gacacgccat cttgaggtgg tggtggcgca agccgtgggg gttcaagtcc ccccctccgc
     6961 accaaatgat taatttggat atagggattt gttaagggat aacaaattag tttaagttaa
     7021 agcaaaataa agcttgactt gttgaggtga agtctgtaaa gttcgcctcc tgtcgttgcg
     7081 gggtggagca gtctggtagc tcgtcgggct cataacccga aggtcgttgg ttcaaatcc
//
LOCUS       DAOQMB010000078         7115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4369_length_7115_cov_14.688385, whole genome shotgun sequence.
ACCESSION   DAOQMB010000078 DAOQMB010000000
VERSION     DAOQMB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4369_length_7115_cov_14.688385"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLY05_04330"
     CDS             complement(<1..629)
                     /locus_tag="PLY05_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNC02562.1"
                     /translation="MNVLIIHAHPEAKSFNTALMQLATEQLTAAGHQVQVSDLYEMNW
                     NPVASANDFAQRKNPDYLVYALEQRHNYEQQAIAPDIAAELEKLKAADLVIFNFPLYW
                     FSVPAILKGWIDRVLISGVCYGGMRFYDQGGLKGKKALVTLTLGGREHMFGEKAIHGE
                     LVDGMLRPLLRGTLAYVGFEVLPPFIGYHVPYIKPEAREQILVDYKNYLA"
     gene            835..1710
                     /locus_tag="PLY05_04335"
     CDS             835..1710
                     /locus_tag="PLY05_04335"
                     /inference="COORDINATES: protein motif:HMM:NF006109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNC02563.1"
                     /translation="MTYQTLLYQVENRILTITLNRPEQMNAFTVEMANELEHAFKTAS
                     DDDNIGAIVVTGAGKAFCAGMDLSKQGNVFGLNEQLQPTTADMEQRLDDETMIAGVRD
                     TGGRVTLAIFDCKKPVIGAINGAAVGIGATMTLAMDIRLASSKARIGFVFGKIGVVPE
                     ACSSWFLPRIVGISQALEWVYSGDILTAEEAQQGRLVKAVYSPETLLEEAYKLAHKFI
                     DNRSPVAIAFARQMMYRNAAQTHPIEAHKVDSLAIFYTSLEDGKEGVKSFLEKRAPEF
                     TGKASQMPEFYPWWK"
     gene            1830..3869
                     /gene="rep"
                     /locus_tag="PLY05_04340"
     CDS             1830..3869
                     /gene="rep"
                     /locus_tag="PLY05_04340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HNC02564.1"
                     /translation="MPTMNNLNPRQQEAAKYIDGPLLVLAGAGSGKTSVITKKIAYLI
                     TECGIKANRIFAVTFTNKAAKEMKERVSKLVNGAEAKGLTVSTFHTLGLTMIRQELEA
                     CGLKNNFSILDADDCQRLLADLMHKDNSPEADQKQALKWISKKISDWKNDLIAPVQAV
                     SVAETKEDLIAAHLYDAYQRHLRAYNAVDFDDLIALPTRILQENQAIREKWQSKVHYM
                     LVDEYQDTNTAQYLLVKLLVGVRGRFTAVGDDDQSIYAWRGARPENMLELTKDYPNLK
                     VIKLEQNYRSMGRILNAANSVIANNPHVFEKKLWSQHGLGDLIRVVTCETDRQEAEYV
                     ANDLVNNRLMNRRTFKDYAILYRGNFQAKAIETQLRELQVPYKLSGGTSFYARAEIKD
                     VMSYLRLIINPDDDSAFLRIINTPKRAIGPVTLEKLGVYAQQRGVSLLMACTELGLAS
                     HLGQKKAVVSLHEFGTWLEEHSKVILDNTDPVPKIRQLLSDIAYEDYIRETAPTPQGA
                     ESRIENINFLLNSIQNMLNKADDEDDKNIEAIIRKLVLIDLLEQQAENDDSDRVQLMT
                     LHAAKGLEFPHVYMIGLEEDLLPHRNSIDADTVEEERRLMYVGITRAKQTLTITLAER
                     RKSGADFKSTTPSRFLEELPADQLEWQGRRSQKSPQEKKRIGNAHLANLRALLNS"
     gene            3882..4082
                     /locus_tag="PLY05_04345"
     CDS             3882..4082
                     /locus_tag="PLY05_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HNC02565.1"
                     /translation="MTDEMLTKWGMQLGIPALILFLMFIIWDIAKKSNAGKTGTFALF
                     LALSVGFVGYMIKVILQWQLEK"
     assembly_gap    4083..4182
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4183..4689)
                     /locus_tag="PLY05_04350"
     CDS             complement(4183..4689)
                     /locus_tag="PLY05_04350"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNC02566.1"
                     /translation="MNKYAVSAIGLLILIGVNGSIWQKEQVLSNGQTVFLTLAPVDPR
                     SIMQGDYMRLRFELESQVRAQLPQDQTENSQGFVLVKLDDKGIAQFINLQTEPTIIES
                     HTAALQYRVREGKIKFATDAFFFEEGQGDIYAQAKYGEFKVAENGELLLTDLRGENLI
                     KLNKKALD"
     gene            complement(4682..5740)
                     /locus_tag="PLY05_04355"
     CDS             complement(4682..5740)
                     /locus_tag="PLY05_04355"
                     /inference="COORDINATES: protein motif:HMM:NF025709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4401 domain-containing protein"
                     /protein_id="HNC02567.1"
                     /translation="MSNQHDAALWQKLQEAGLVQGDMPNTDIENHPWYLRSLQGFTGW
                     FAAWFVLASIFALFYKVLEYKELALVFGIATLSGAYFIYQKAQKSDFIGQFALAISLT
                     GQSLCIYAWRDLLHDPIGWWLIAFMSACLVWLMPSFIHRFLSSSMAIGAILLGCMDWP
                     IVWLLPSIVVVAMAAVWLAESKWLIHSDRVRPIAYALTISALQIHSLTLLGVNGLGQL
                     IHGRDLPAGSFLLAVNEVLLGFALVATVGMLLKRYQIGYQQPQAWLALLLATVLGLVS
                     QQATGLAVAWILIVIGFANGHRVLLALGVFVFWGYLARYYYFLNWTLLDKSYLLMATG
                     AFLLIVRQIGQRWRQHHE"
     gene            complement(5733..6710)
                     /locus_tag="PLY05_04360"
     CDS             complement(5733..6710)
                     /locus_tag="PLY05_04360"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNC02568.1"
                     /translation="MTPARHKALIKQWLSQKHIAPDKTEQALAIATVTPPASAWFGFL
                     DRLLLSNGVGLILIGLIFFFAFNWQEITRFHKFAMLELLIVGTLVAHYKFAAKANANY
                     LILVASVLLGVLLAFYGQTYQTGADTWQLFAMWALLISPWVLLARFAALWMLWLILAN
                     LSLVLYYQVFHGLFGLVFSGTAMQWALWLFNTLALIIWEFAAKRYSWLSQSRSQVRLL
                     AIVTHFNMVWLMIDFIFSHGFRDFYLPIIYLAWLAAFYGYYRYQQLDVFMLAGVSLSV
                     IIISTAWFSDLLLQSKDGFALLVIGLWIMFSSGWAVRHLKAIAQEVPHE"
     gene            complement(6744..6968)
                     /locus_tag="PLY05_04365"
     CDS             complement(6744..6968)
                     /locus_tag="PLY05_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02450 family Trp-rich protein"
                     /protein_id="HNC02569.1"
                     /translation="MTRRLNPKKLLLSKWTAVNPQQQEKHFLVTQIANNSTPIAERDL
                     IILEAVMSKNAYRIDWHELLDEVTWRQGWH"
BASE COUNT         2261 a         1509 c         1311 g         1934 t
ORIGIN      
        1 gctaaataat ttttataatc caccaaaatt tgttcgcgtg cttcgggttt aatatacggc
       61 acatgatagc caataaacgg tggcaacacc tcaaaaccca catacgctaa agtaccacgc
      121 aacaagggac gtaacatacc atccaccaac tcaccatgaa tagctttttc accaaacata
      181 tgttcacggc cacccaaggt taaagtcact aaagcttttt taccttttaa gccaccttgg
      241 tcataaaaac gcattccgcc atagcacacc cctgagatta acacacggtc aatccaccct
      301 tttaaaatcg caggcacaga aaaccaatat aacggaaagt taaaaatcac caaatccgcc
      361 gcttttaact tttctaactc agccgcaata tcaggtgcaa ttgcttgttg ttcgtagtta
      421 tgacgttgtt ctaaggcata caccaaataa tcaggatttt tgcgctgggc aaaatcgtta
      481 gcagaagcca caggattcca attcatctca tataaatcgg acacctgtac ttgatgacca
      541 gcagcagtta attgctctgt cgcaagctgc attaatgccg tattaaagga ttttgcttca
      601 ggatgggcgt gaataatcaa gacattcatc atttatgctc aatttaaact aaaaatgggc
      661 ttgatttaag caccaaatta ctcgtataag caactcagtt aagtaatttt tggtttcaac
      721 ccttcgacag gctcagggct tgccaagctc aaccaccaca aacgttccct gagcagtggt
      781 cactgagcga agccgaagtg agtcgaaggg tgatttttag gagcaaataa caacatgact
      841 tatcaaaccc ttttatatca agtcgaaaac cgtattttga cgattaccct caaccgtccc
      901 gaacaaatga atgcctttac cgttgagatg gccaatgaat tagaacacgc atttaaaact
      961 gccagtgatg atgacaatat tggcgcgatt gtcgtaacag gtgctggcaa agctttttgt
     1021 gctggcatgg atttaagcaa acaaggtaat gtctttggtt tgaatgaaca attacaaccc
     1081 acaacagcgg atatggaaca acgcttagat gatgagacaa tgattgcagg ggtacgagat
     1141 acaggtggtc gtgtcacctt ggctattttt gattgcaaaa agcctgtgat tggtgcgatt
     1201 aatggcgcag ccgtaggcat tggcgcaacc atgaccttgg caatggatat tcgtttagcc
     1261 tcctctaaag cacggattgg ttttgttttt ggcaaaattg gcgtagtacc agaagcctgt
     1321 tcaagttggt ttttgccgcg tattgtgggc attagtcaag ctttagaatg ggtgtatagt
     1381 ggtgatattt taactgccga agaagctcaa caaggccgtt tagtcaaagc ggtttatagc
     1441 ccagaaacct tgttagaaga agcttataaa ctcgcccata agtttataga taatcgttca
     1501 cctgttgcta tcgcctttgc tcgacaaatg atgtatcgca atgctgcaca aacacatccc
     1561 attgaagctc ataaagtaga ctctttggca atattttaca ccagtcttga agatggcaaa
     1621 gaaggtgtaa aatccttttt agaaaaacgc gcccctgaat ttacaggcaa ggccagccaa
     1681 atgcctgaat tttatccttg gtggaagtaa aatctctaaa ttgaggcatt ttttaaatgc
     1741 ctccgttttt gttacaaacc ctataaagtt tatttgcacc tgccccacct tcttcgtaaa
     1801 atagcacgcc ttcccttttt ctaaactgca tacccaccat gaacaaccta aatccgcgtc
     1861 aacaagaagc cgctaaatat attgatggcc cattattagt tttggcgggt gcaggctcgg
     1921 gcaaaacctc ggtgattacc aaaaagattg cctatttaat taccgaatgt ggcatcaaag
     1981 ccaatcgtat ttttgcggtg acctttacca acaaagccgc aaaagaaatg aaagagcggg
     2041 ttagcaaatt agttaatggg gcagaagcca aaggcttaac cgtctccacc tttcatacct
     2101 taggcttgac catgattcgc caagaattag aggcttgtgg tttaaaaaat aatttttcta
     2161 ttttagatgc agacgattgc caacgtttat tggccgattt aatgcacaaa gataacagtc
     2221 ctgaagctga ccaaaaacaa gcattaaaat ggattagcaa aaagatttct gattggaaaa
     2281 atgatttaat cgcgcctgtt caagcagtga gtgttgccga aactaaagaa gatttgatag
     2341 cagcccatct ttatgatgcc tatcaacgcc atttacgcgc ttataacgct gtagattttg
     2401 atgacttgat tgccctaccc acacgtatct tgcaagagaa tcaggccatt agagaaaaat
     2461 ggcaaagtaa agtgcattat atgttggtgg atgaatatca agacaccaac accgcccaat
     2521 acttattagt caagttatta gtgggtgtgc gtggtcgttt taccgccgtg ggtgatgatg
     2581 atcaaagtat ttatgcgtgg cgtggtgcgc gtcccgaaaa tatgttggaa ctcaccaaag
     2641 attaccccaa tttaaaagtc attaaacttg agcaaaatta tcgctctatg ggacgtattc
     2701 ttaatgctgc caactcggtc attgccaata atcctcacgt ttttgaaaag aaattatgga
     2761 gtcaacatgg gcttggcgat ctaattcgcg tagtaacttg cgaaaccgat cgccaagaag
     2821 cggaatatgt agccaatgat ttggtaaata accgtttaat gaatcgccgc acctttaaag
     2881 attatgccat tttgtatcgt ggtaattttc aggccaaagc cattgaaact caattacgcg
     2941 aactgcaagt gccttataaa ttatcaggcg gcacatcctt ttatgcacgt gccgaaatca
     3001 aagatgtgat gagttattta cgcctgatta ttaaccctga tgatgacagt gcttttttac
     3061 gcattattaa tacgccaaaa cgtgcgattg gccctgttac tttagaaaaa ttaggcgttt
     3121 atgcccaaca acgtggcgtg tctttattaa tggcttgcac agagttaggc ttagcttcgc
     3181 atttaggcca aaagaaagct gtggttagtt tgcatgaatt tggcacgtgg ctcgaagaac
     3241 acagcaaagt gattttagat aacaccgacc ctgtgccaaa aattcgccaa ttattaagcg
     3301 atattgctta tgaagattat attcgtgaaa cagcacccac gcctcaaggt gcagaatcac
     3361 gtatagaaaa tatcaatttc ttgctgaact ctattcaaaa tatgttgaat aaagctgatg
     3421 atgaagacga caaaaatatc gaagcgatta ttcgcaaatt agttttaatt gatttactcg
     3481 aacagcaagc cgaaaatgac gatagcgacc gtgtgcaatt gatgacgcta catgccgcaa
     3541 aaggcctaga gtttcctcat gtgtatatga ttggtttaga agaagatttg ttgccacatc
     3601 gcaatagtat tgatgctgat accgttgaag aagaacgtcg attaatgtat gtgggtatta
     3661 ctcgtgccaa acaaacctta accattactt tagccgaacg acgcaaatcg ggtgcagatt
     3721 ttaaaagcac cacgccgagt cgctttttag aagagttacc tgctgatcaa ttagaatggc
     3781 aaggacgacg cagccaaaaa tctccgcaag agaaaaagcg tattggtaac gcccatttag
     3841 ccaatttaag agccttgtta aattcttagt gagaaaccaa catgacagat gaaatgttaa
     3901 ccaaatgggg aatgcaatta ggtattcctg ctttaatttt gtttttgatg tttattattt
     3961 gggatattgc caaaaagtct aatgcaggca aaacaggtac ttttgcgcta tttttagcct
     4021 tatctgtagg ctttgttggc tatatgatta aagttatttt gcagtggcaa ttagagaagt
     4081 aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttaatcca aagccttttt
     4201 attgagtttt attaaatttt caccacgcaa atcggttaat aacaactctc cgttttctgc
     4261 cactttaaac tccccatatt tagcttgggc ataaatatca ccttgccctt cttcaaagaa
     4321 aaaagcatca gtcgcaaatt taatttttcc ctcacgaaca cgatattgca aagcggctgt
     4381 atgagactca ataatcgttg gttctgtttg caagttaata aattgagcaa tccccttatc
     4441 atccaattta accaacacaa agccctgact attttcagtt tggtcttgcg gcaattgggc
     4501 gcgtacttgg ctttctagtt caaagcgtaa acgcatataa tcgccttgca taattgagcg
     4561 aggatcaaca ggtgctaaag ttaaaaatac cgtttggccg ttacttaata cttgctcttt
     4621 ttgccaaata gacccattta cgccaattaa aatcaataaa ccaatcgcgc ttaccgcata
     4681 tttattcatg gtgttgcctc caacgctggc caatttgacg aacaatcaac aaaaatgcgc
     4741 ctgttgccat taacaaatat gacttgtcta ataatgtcca atttaaaaaa taataataac
     4801 gcgccaaata tccccaaaaa acaaaaacgc ccaaggccaa caacacccga tggccattgg
     4861 caaaaccaat gacaattaaa atccacgcca ctgctaaacc tgttgcctgc tgtgaaacta
     4921 agcccaatac cgtcgccaac aataaggcca accatgcttg tggttgttga tagccaattt
     4981 gataacgctt taatagcatg cctaccgttg caactagggc aaaacccaac aacacttcat
     5041 taaccgccaa taagaagcta cctgctggta aatcacggcc atgtatcaac tggcctagac
     5101 cattaacccc taacagcgtt aagctatgaa tttgcaacgc actaatggtt aatgcataag
     5161 caattggacg tactcgatca ctgtgtatta accatttact ttctgctaac caaacggcag
     5221 ccattgccac cacaacaata ctgggcaaca accacacaat tggccaatcc atacaaccca
     5281 gtaatattgc gccaattgcc attgagcttg ataaaaaacg atgaataaaa gacggcatta
     5341 accacactaa acaggccgac atgaaggcaa ttaaccacca gcctatgggg tcgtgtaata
     5401 aatcacgcca cgcataaata cataaacttt ggcctgttaa actaattgct aaggcaaact
     5461 gaccaataaa atcacttttt tgtgcttttt gataaataaa atacgcgcct gacaaggttg
     5521 cgataccaaa gactaacgcc aactctttgt attcaagcac tttataaaac aaagcaaaaa
     5581 tagaagccaa aacaaaccat gcggcaaacc aaccagtaaa gccttgcaaa ctgcgtagat
     5641 accaagggtg attttctatg tccgtgtttg gcatatcgcc ttgcaccaac cccgcttcct
     5701 gtagtttttg ccataaggca gcatcatgct gattactcat gtggaacttc ctgtgcgatt
     5761 gcttttaaat gacgaactgc ccaccctgaa ctaaacatga tccataaacc aatgaccagc
     5821 aaggcaaatc catctttgct ttgtaataac aaatctgaaa accatgcagt tgaaataata
     5881 atcacgctta aactaacacc agccagcata aaaacatcaa gttgttgata acgataatag
     5941 ccataaaaag ctgctaacca tgccaaatag atgatgggca gataaaaatc tctaaagcca
     6001 tgactaaaaa taaaatcaat catcagccag accatattaa aatgagtaac gatggctaat
     6061 aaacgcactt gactacgact ttggcttaac catgagtaac gcttggcggc aaattcccaa
     6121 ataatcaaag ccaatgtatt aaataaccac aacgcccatt gcatggctgt gccactaaat
     6181 accagtccaa ataaaccatg aaacacttga taatagagca ctaagcttaa gttagctaaa
     6241 atgagccata acatccataa cgctgcaaat cttgctaaca aaacccaagg gctaattaat
     6301 aatgcccaca tcgcaaatag ttgccacgta tctgcacctg tttgataggt ttgaccataa
     6361 aaagccaaca acacccccaa taaaacagaa gcgaccaaaa tcaaataatt agcattcgct
     6421 tttgcggcaa atttataatg agccactaaa gtgccaacaa ttagcagctc taacatcgca
     6481 aacttgtgaa aacgagtgat ttcttgccaa ttaaaggcaa agaaaaaaat cagtccaatt
     6541 aaaattaaac caacgccgtt acttaacaac aaacgatcta aaaaaccaaa ccatgctgag
     6601 gctggtggtg tgactgttgc aatggctaac gcttgttctg ttttgtcagg ggcaatatgt
     6661 ttttgactga gccattgttt aatcaaggct ttatggcggg ctggtgtcat ctacagactc
     6721 cttgcggcga tcatcaggcg ttattaatgc catccttgtc gccatgtgac ttcatctaat
     6781 aattcatgcc aatcgatacg ataagcattt ttgctcataa cggcttctaa gataatcaaa
     6841 tcacgctctg ctattggcgt ggaattattg gcaatttggg tgactaaaaa atgtttttct
     6901 tgttgttgtg ggttcactgc tgtccattta gacagcagca gtttttttgg attcaatcga
     6961 cgagtcatat taaaacttag gcttttgggt taaacgttcc caccatttta acaacacatt
     7021 gtccaaagta ttgctagccg ttaaacctac tttttgcata aagttttgtt tatgaataaa
     7081 acgtactgca aaaatatcag ctgttttact taaac
//
LOCUS       DAOQMB010000079         7042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4444_length_7042_cov_15.285673, whole genome shotgun sequence.
ACCESSION   DAOQMB010000079 DAOQMB010000000
VERSION     DAOQMB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7042
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_7042_cov_15.285673"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PLY05_04370"
     CDS             <1..555
                     /locus_tag="PLY05_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HNC02570.1"
                     /translation="LDPDLRATVHQVLACPYFRVFANTDIYGVELSGALKNIYAIATG
                     MAQANKVGENTRSMLLTRALAEMSRFAVQLGANPLTFLGLAGVGDLFATCSSPLSRNY
                     QVGFALGSGKKLNDIIADLEQTAEGINTIKQVKQQADKLGVYMPIVSGLYEVIYNEKP
                     IAEVAAKMMNSGQRSDVEFVLPNR"
     gene            561..1028
                     /gene="sixA"
                     /locus_tag="PLY05_04375"
     CDS             561..1028
                     /gene="sixA"
                     /locus_tag="PLY05_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HNC02571.1"
                     /translation="MQIILIRHGQAESLKTTDAARALTSEGEFQAQQTAAWLVNHDYQ
                     LDALIVSPYIRAQQTAYHIAQAFDLPMTICDKITPEDEPKQAFEWLDDLMLPDSAVVA
                     VVCHMPIVARLASLLTGEPIHLISGFDLAEAQVLDMPIFGLGMAKVLAKFIPN"
     gene            complement(1080..1292)
                     /locus_tag="PLY05_04380"
     CDS             complement(1080..1292)
                     /locus_tag="PLY05_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02572.1"
                     /translation="MITIEITNLDELVKEHRGPVTALLGKIVADVEGEVEKIIMEEIR
                     NEFERRGVRANFCSIEGIRLRRIITA"
     gene            complement(1399..>2100)
                     /locus_tag="PLY05_04385"
     CDS             complement(1399..>2100)
                     /locus_tag="PLY05_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNC02573.1"
                     /translation="FRYITKMGSQQYQQQHPSSLAYFWLNSLESLLNAFVELDPTTRH
                     QLVQLEGIVVRVKTFAPYQVFYLLFTSHGIEVSPQPQGEVQIRLSGKVRDLVWTFLGL
                     ESEQYAESKGHLHLWGDSTLLQQLRGLLQEFNIRSAIAHWLKSHLPLNALWHKLTEQD
                     LSWLRDLQPIPELIKQTRQEIAVLNSELQQQKQQINELQSLVKQQLSYSQSLLKLSIA
                     LIVLSSSLIILNFIH"
     assembly_gap    2101..2200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2230..2655
                     /locus_tag="PLY05_04390"
     CDS             2230..2655
                     /locus_tag="PLY05_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HNC02574.1"
                     /translation="MTTDNKSGDKPRKLSSAAELRKYTQDIWLAGLGAFSRAEEEGGK
                     MFDNLVKVGAELEAKTRDIADNTVETVVEARDKVLEKASDTREKVEKAFDDKLSAALG
                     RLGIPNQRELDSINQRLDTLTQVLQQLTDQMRENQKSQK"
     gene            complement(2700..3644)
                     /locus_tag="PLY05_04395"
     CDS             complement(2700..3644)
                     /locus_tag="PLY05_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC02575.1"
                     /translation="MTQNTQLPCILITGAAGALAQQVIHRLQGKYRLVVCDSRREVSM
                     PEDIPSYRLDFNKRRFEDLFKEYHFEGVIHLGRIGSNENNREGRYAANVIGSQRLLDL
                     ALKYGVKQTLILSTYFVYGAHPYNPAYLTESAPLKAAGITMDLVDSVELENLSNIYLW
                     KYPELNITILRPCNIVGPGVNNTLSQLLMQKRVPALMGYSPMMQFIHLEDMADAIVVA
                     FDKNHAGIYNVAPDDCVPYQVAVKHCGCKILPIPSIPENMPLWLAKISQKDLLPPHLL
                     DYFKYAATIDGSAFSKTFDFQPRYGLKDIFAHYRNLKS"
     gene            complement(3647..4468)
                     /locus_tag="PLY05_04400"
     CDS             complement(3647..4468)
                     /locus_tag="PLY05_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC02576.1"
                     /translation="MKMLSDKLKNWLLPSELKAAIDNAPKPVGSFDYDRWGYHRETAY
                     KAAASVRFLYDHYFKVKAYGQEHIPAQGRVLIVANHSGYLPLDGALLGYSLLTNPYAP
                     RVPRAMMERLFTDVPYVGNLLNQIGAVTGERQNCIDMLNQEEAILVFPEGIRGTSKGF
                     ARRYQLQRFGNGFMDIAIETNTPIIPVGIVGCEEALPMFGNAKGLAKKFGVPYFPITL
                     PLPLPVQVSLNFGEPMYFNGPIHCEDDLEAKVNIVKAKIRQLIADAQHKHGTGSV"
     gene            4711..4983
                     /locus_tag="PLY05_04405"
     CDS             4711..4983
                     /locus_tag="PLY05_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNC02577.1"
                     /translation="MNKSELIDAIAASAQLTKADAARALDGFTNAVTNALKAGDSVAL
                     VGFGTFSVKERAARKGRNPKTGQEIEIAASKVPDFKAGKGLKDAVA"
     gene            5063..6961
                     /locus_tag="PLY05_04410"
     CDS             5063..6961
                     /locus_tag="PLY05_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC02578.1"
                     /translation="MQAFRDLVRGWLGKALLILLLVPFALVGIESYFVGGKAAPAATV
                     NGDEISQFELDKSVDQQKQRILAGMGENPDASLIDTTVLRDEVLKGLVDRALLSTQAK
                     KVGFLIDDETINRLIHETPAFQENGVFSQARYEQMLRNIGEDPLNFPQKAKKEVAVSQ
                     LAGGINQTAFVTSKQLDQLSALDNQKRDVHIAVFPAANYLATVQVTDAEVEQHYKANT
                     TKYTTEEKVAVDYLVLGSELFLDKVLLTDADVEARYAEKIKTLESNEQRRAAHILIKI
                     TDKVKDADALAKIQEIEKKVKAGEDFGVLAKQYSQDEGSAVANGDLGFVGKGAFVPEF
                     EKTMYSLKENEVSAPVKTQFGYHIIKLLAIQKAPVPTMAEIRPELEADVKVAKAEELY
                     AEAIEKMDALAYESADLSEGAKQYGMSVQTSPLFSKNGGEGLAANRKIVQAAFSDDIL
                     KDGKNSQALALDNKHSVWIHLNKHEPARVKPLTEVAAEIKTNLQLDKAALIAKQKAEE
                     MTKALNAGKSAAELAAANQLQWQDFLSSARNAPVPAANLLKVAFRLAVPKANTWTAAL
                     APSGKDYAVVAVSKVDAGTSTLTDDQKKQFAAMLANARGQQDLQDYVTYLRSHAKIEK
                     PDVKSTDK"
BASE COUNT         2176 a         1229 c         1457 g         2080 t
ORIGIN      
        1 ttagaccccg atttacgtgc tactgttcat caagtgttag cttgcccata ctttagggta
       61 tttgctaata ctgatattta tggtgttgag ttaagtggcg cgttaaaaaa tatttatgcc
      121 attgcgacag gtatggcgca agccaataaa gtgggcgaaa atactcgtag tatgttactg
      181 acacgagctt tagcagaaat gagtcgtttt gctgtgcaac tgggtgcaaa ccctttaacc
      241 tttttaggtt tagcaggggt tggtgattta tttgcaacct gtagttcccc tttaagtcga
      301 aattatcaag ttggttttgc cttaggttct ggtaaaaaat taaatgatat tattgctgac
      361 ttagagcaaa ccgccgaagg tattaacacc attaaacaag tgaaacaaca agccgataaa
      421 ttgggcgtat atatgccgat tgtatcgggt ttatatgaag tcatttataa tgaaaagcca
      481 attgccgaag ttgctgctaa aatgatgaac agtggtcaac gtagtgatgt agagtttgtt
      541 ttacctaatc ggtgagtttt atgcagatta tcttaatacg tcatggtcaa gcagagtctt
      601 taaaaacgac agatgcagca cgtgctttaa cctccgaagg agagtttcag gcgcaacaaa
      661 ctgctgcatg gttagttaat catgattatc aattagatgc cttgattgtt agcccttata
      721 tacgagcgca acaaactgct tatcatattg ctcaagcttt tgatttgccg atgacgatat
      781 gtgacaaaat taccccagag gatgaaccta aacaagcatt tgaatggtta gatgatttga
      841 tgttgcctga ttcggcagtg gtggctgtcg tgtgtcacat gccaattgtt gcacgtttag
      901 cttcgttatt gacgggtgag cccattcatc ttatttcagg ttttgattta gcggaagcgc
      961 aagtattaga tatgcctatt tttggattgg gaatggcaaa agtattggca aagtttatcc
     1021 ctaactaaac gtagtaacag caaaaagact tacgcagtct tgcgtaagtc ctaaggtact
     1081 taagcagtaa taattcggcg taaacgaatg ccttcaatac tacaaaagtt tgctcttacc
     1141 cctcgacgtt caaactcatt acgaatttct tccataataa ttttttcgac ttcaccttca
     1201 acatcagcca caatcttccc caatagagcg gtcactgggc cacgatgttc tttgactaat
     1261 tcatctagat tggtaatttc gatagtaatc atattgtccg tctgcttagg ttaatgagac
     1321 acttgtttat ttcatctcaa caatcagtcg ctgacaatga ctattatcag caaaagtgta
     1381 aaagaagatg acggtatatt aatgaataaa gtttaatata atcaagctgc tacttaaaac
     1441 aattagcgca atacttaatt ttaataaaga ttgactatag ctaagttgtt gtttaactaa
     1501 actctgtaac tcattgattt gttgtttttg ttgttgtaat tcgctgttca gcacagcgat
     1561 ttcttgcctt gtttgtttaa ttaactcggg aatgggttgt aaatcacgca gccacgacag
     1621 gtcttgttct gttaatttgt gccacaaggc attgaggggt aaatgactct ttagccaatg
     1681 tgcaattgcc gaacgaatat taaactcttg taacagccca cgtaattgtt gcaataaagt
     1741 actgtcaccc cataaatgta agtggccttt agattcggca tattgttcgg actctaaacc
     1801 taaaaaagtc caaactaaat cacggacttt accgcttaga cgaatttgta cttcaccttg
     1861 aggttgtggg ctgacttcaa ttccatgact agtaaatagc aaataaaaaa cctgataagg
     1921 tgcaaaagtt ttaactctga cgacaatgcc ttctaattgt actaattgat gacgtgtggt
     1981 tgggtctaat tcgacaaaag cattgagcag gctttctaaa ctatttagcc aaaaataagc
     2041 aagtgatgaa ggatgttgtt gttgatattg ttgactgccc atctttgtta tatacctaaa
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ttaaagtaat ttgggctatt
     2221 gggggatata tgacaactga caataaatcg ggtgataaac cacgtaaact gtcatctgct
     2281 gctgaattgc gtaaatatac gcaagatatt tggctggcag gcttgggggc tttctctcgt
     2341 gccgaagaag aaggcggaaa aatgtttgat aacttggtaa aagtaggtgc cgagttagaa
     2401 gcaaaaacac gtgatattgc ggataacaca gtagaaactg tggttgaagc acgtgataag
     2461 gtgttagaaa aagccagtga tactcgtgaa aaagtagaaa aagcttttga tgacaagtta
     2521 tctgctgctt tgggtcgttt gggtattccc aatcaacgtg aattagattc tattaatcaa
     2581 cgtttagata ccttaacgca agtattgcaa cagttaaccg atcaaatgcg tgaaaatcag
     2641 aaatcacaaa aataatcttt gtgaagaaaa aaacagccca taattgggct gtttttgttt
     2701 tagcttttta aattgcgata atgggcaaaa atatctttta aaccataacg aggttgaaag
     2761 tcgaaagttt tgctaaaggc agagccatca atggttgcag catatttaaa atagtctaat
     2821 aggtgggggg gtaataagtc tttttggcta attttggcta gccataaggg catattttcg
     2881 ggaatagagg gaatcggtaa gattttgcag ccacaatgtt taacggctac ttgataaggc
     2941 acacagtcat caggtgcaac gttgtagatg cctgcatgat ttttatcaaa agcgacaacg
     3001 atggcatctg ccatatcttc caagtgaata aactgcatca ttggtgaata gcccattaat
     3061 gcaggaacgc gtttttgcat taacagttga cttagcgtgt tgttaacacc aggccctacg
     3121 atattacatg ggcgtaaaat cgtaatattc agttctggat atttccataa ataaatattg
     3181 gataagtttt ctaattcaac agaatcaacc aagtccatgg taatgcctgc cgctttgagc
     3241 ggtgcacttt cagttaaata agctgggttg tagggatgtg cgccatagac aaaataggtc
     3301 gataaaatca gggtttgttt aacaccatat tttagagcta agtctaacaa acgttgactc
     3361 ccaatgacat tggcggcata acggccttca cggttatttt cgtttgagcc aatacgtccc
     3421 aaatgaatca caccttcaaa atgatattct ttaaataaat cttcaaaacg acgtttgtta
     3481 aaatcaagac gatagcttgg aatatcttcg ggcatactga cttcgcggcg gctatcgcag
     3541 accactaaac ggtatttacc ttgcaaacga tgaatcactt gttgtgcgag tgcgcctgct
     3601 gcacccgtaa ttaaaataca gggaagttgg gtgttttgtg tcatttttat actgatcctg
     3661 ttccgtgttt atgttgcgca tctgcaataa gttgacgaat tttggcctta acaatattca
     3721 cctttgcttc taaatcgtct tcacagtgaa ttggcccatt aaaatacata ggctcaccaa
     3781 aatttaagct aacttgcaca ggtagaggca atggtaaggt aattggaaaa taaggtacgc
     3841 caaatttttt agctagacct ttggcattgc caaacatcgg taaggcttct tcacaaccaa
     3901 caatgccgac tggaataata ggcgtattgg tttcaatggc aatatccata aaaccattgc
     3961 caaaacgttg cagttgataa cgacgagcaa aacctttact agtaccacga ataccttcag
     4021 gaaatacgag aatagcttcc tcttgattga gcatatcaat acagttttga cgttcgcctg
     4081 ttactgctcc aatttggttt aataaattgc caacataagg gacatcagtg aaaagacgct
     4141 ccatcatggc tcgtggtaca cgaggcgcat aaggatttgt taataagcta tatccaagta
     4201 atgcgccatc taaaggtaaa taacctgaat ggttggccac aatcaaaact cgaccttgag
     4261 caggaatatg ctcttggcca taagctttaa ctttaaagta atgatcatat aaaaagcgaa
     4321 cagacgcagc agccttatat gcggtttcac gatgatagcc ccaacggtca taatcaaaag
     4381 agcctacagg cttgggagca ttgtcgattg ctgctttgag ttcggaggga agtaaccagt
     4441 ttttgagctt gtcggagagc attttcatct tccaaaaata ggcgagtaac gatttacaat
     4501 actaaattac cgcaaataat ttgctaagtg aaacacaaaa attgtttttt agcgattaaa
     4561 tacaatagtc ctactatgtt atccttgacc accgcctatg agcattggta taaatcgcca
     4621 ccttacaaat aaaccagcta atgtgataca gtgcgggcta tcgccgtatt ttaatagcat
     4681 cgtgagttag acaataacta aggggaaaaa atgaacaagt ccgaattaat tgacgcaatt
     4741 gccgcttccg cacaattaac taaagcagat gctgctcgtg ctttagatgg ttttactaat
     4801 gctgtgacga atgctttaaa agcaggagat tcagtggctt tagttggctt tggaacattc
     4861 agtgtcaaag agcgtgctgc acgtaaaggc cgtaatccaa agacaggcca agaaattgaa
     4921 attgcagcaa gcaaagtacc tgattttaaa gcaggtaaag gcttaaaaga cgctgtcgct
     4981 taagtggtta ttgctttgca aataacaagc gcgcccattt atgggcgcgt tttattttag
     5041 attttgctgt ttggtgaatt tgatgcaagc ctttcgtgat ttggtgcgag gttggctagg
     5101 taaagccttg ctaattttgc tattagtccc atttgcttta gtgggtattg agtcctattt
     5161 tgtcggaggt aaagcagcac ctgcggcgac agtaaatggc gatgaaatta gtcaatttga
     5221 gttagataaa agtgtagatc aacaaaagca acgtattttg gcaggtatgg gcgaaaatcc
     5281 tgatgcttct ttaattgata cgactgtttt acgtgatgaa gtattaaaag gtttagttga
     5341 tcgagcctta ttaagtactc aagctaaaaa agttggtttt ttgattgatg atgaaaccat
     5401 taatcgcttg attcatgaaa cacctgcgtt tcaagaaaat ggtgtatttt cgcaggctcg
     5461 ttatgagcaa atgttgcgta atatcggcga agacccacta aactttccac aaaaagctaa
     5521 aaaagaagtg gctgtttcgc aattagcggg tggtatcaac caaacggctt ttgtaacatc
     5581 taaacaatta gatcaattat ctgctttaga taatcaaaag cgtgatgtgc atattgccgt
     5641 gtttccggct gctaattatt tagcaactgt acaagtgact gatgcagagg ttgaacaaca
     5701 ttacaaagcc aatacaacaa aatacacgac tgaagaaaag gttgccgttg attatttggt
     5761 tttaggctca gagttatttt tagacaaagt attgttaaca gatgctgatg ttgaggctcg
     5821 ttatgccgaa aaaatcaaaa ctttagagtc taatgagcaa cgtcgtgcag cccatatttt
     5881 gattaaaatt acagataaag ttaaagatgc tgatgcttta gcaaaaattc aagaaattga
     5941 gaaaaaagtt aaggcaggtg aggattttgg tgttttagca aaacaatatt ctcaagatga
     6001 aggttcagcc gttgctaatg gtgatttagg ttttgttggt aaaggtgctt ttgttcctga
     6061 gtttgaaaaa accatgtatt ctttaaaaga gaatgaagtg tctgcgccag tcaaaacaca
     6121 atttggctat cacattatta agttactagc tattcaaaaa gctcctgttc ctacaatggc
     6181 cgaaattcgt cctgaattag aagctgatgt taaagtcgca aaagccgaag agctatatgc
     6241 tgaagccatt gaaaaaatgg atgctttagc ttacgagtca gctgatttaa gcgaaggtgc
     6301 aaaacagtat ggtatgtcgg tacaaacatc acctttattc tctaagaatg gtggagaggg
     6361 cttagccgct aatcgtaaaa ttgttcaggc agcatttagt gatgatattt taaaagatgg
     6421 caaaaactct caggctttag ctttagataa taaacatagt gtttggattc acttaaacaa
     6481 gcatgagcct gcacgtgtta agccattgac agaagttgct gctgaaatta aaaccaattt
     6541 acagttagat aaagcagcat taattgccaa acaaaaagct gaagaaatga ctaaggcatt
     6601 aaatgcgggt aaatcagctg ccgagttagc tgctgcaaat cagctacaat ggcaagactt
     6661 tttgtctagt gctcgtaatg cgcctgtgcc tgccgctaat ttactcaaag tcgcttttag
     6721 acttgctgta cctaaagcca atacatggac tgctgcatta gctccttcag gcaaagatta
     6781 tgcagttgtt gcagtaagta aggttgatgc aggtacttca acattaacag atgaccagaa
     6841 aaagcaattt gctgctatgt tagcaaatgc tcgtggccaa caagacttac aagactacgt
     6901 cacttattta cgtagtcatg ctaaaattga aaagcctgat gtaaagtcaa ctgataagta
     6961 acgattataa gttttatgca ttgtaccttg tgtaaaactt aaatcagatg ccaaaaaagg
     7021 cagaccaaag gtctgccttt tt
//
LOCUS       DAOQMB010000080         6988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4497_length_6988_cov_15.512332, whole genome shotgun sequence.
ACCESSION   DAOQMB010000080 DAOQMB010000000
VERSION     DAOQMB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4497_length_6988_cov_15.512332"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..328)
                     /locus_tag="PLY05_04415"
     CDS             complement(89..328)
                     /locus_tag="PLY05_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02579.1"
                     /translation="MKLSKAKIAAVIVLVVLLLSAGALYVPDKTVAELSPRWAVAPSQ
                     FMTVDGQQVHIRDEGLKSDPVPIVLLHGTSASLHT"
     gene            complement(325..1575)
                     /locus_tag="PLY05_04420"
     CDS             complement(325..1575)
                     /locus_tag="PLY05_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004656189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC02580.1"
                     /translation="MHEQHIAIIGAGTAGLAAAIILARQGHKITIFEAVDHLSPVGAG
                     LLLQPAGLAVFAHLGVLEHALGLGAVVTGLEGRLPNQQLLVNSHYLQAGQNLYGLGIH
                     RATLCHVLMTALQNQNFTHHITWHMGAHIDKVEEQQGSCVVTGSIQQQAFTTNFDLVI
                     VANGARSQLRPKEWVRLDKSYPWGAAWTIVPECLALDTQILHQFYDGAKIMMGVLPTG
                     AMPDAPTQRLSSVFWSLHSQQLQQWGKTEKQRQDWLTQVDQRWPVVAQWLATVINQPD
                     QWLAANYRDVVMTRFGQGRIGVIGDAAHAMSPQLGQGANMALLDAWALGQACAKATDL
                     NELWQNYHQLRASSTHFYQWLSRQLTPLYQSDNEWAAKLRDFAFIWMYRVPYFRKEMA
                     ITISGLKKGMFTAFDFNDISEYKK"
     gene            complement(1590..3035)
                     /locus_tag="PLY05_04425"
     CDS             complement(1590..3035)
                     /locus_tag="PLY05_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNC02581.1"
                     /translation="MPLAGYYHNELEKVDKASKRLREVMDGLSPAFFIGMLNLEGCVT
                     YANQTALDAVGIERKDVLGRKFDETPWWAHSETQREQLRSAIYKALQGQSSRFDMVFR
                     DINHKLRVIDFSLHPVFDVKNNVSYLVPSGHDVTERRRAERSLSMITECQALLLQVDE
                     EQRLLQNICQLIMDRGGYPHVWIGSAQQDERKTILPVAYVGIEAADFKDYLSWSADDI
                     RGQGLTGPCIRQSKTILCQDFLQVESLAVQQMATSRGIRGGIALALKDHLGNAFGVLS
                     IVSHEVFDIEQEEVLLLEKLADSISYGIRNLRARKENEQSLATIYQIADVVSLATGDH
                     FFQKLTLAVTKILGAEVGFISRVQLDKPSLTRSVALVVDGNLLDNINIPIIGTPCERL
                     TTIHKEWIVSCNVIEQYPLAEGLRMFGAESYIGRRIENSQGEYTGQIVLLFRRPLLDT
                     ALISAVFKVFAARVAAEFERIRQEDDHVYKI"
     gene            complement(3246..3587)
                     /locus_tag="PLY05_04430"
     CDS             complement(3246..3587)
                     /locus_tag="PLY05_04430"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC02582.1"
                     /translation="MSEQIEITDIVLGEGKAVVKGALITTQYTGWLEDGTKFDSSYDR
                     GQAFQCVIGTGRVIKGWDIGLMGMKVGGKRKLFVPAHLAYGERKIGAYIQPNSNLIFE
                     IELLEVLTRDD"
     gene            complement(3597..4139)
                     /locus_tag="PLY05_04435"
     CDS             complement(3597..4139)
                     /locus_tag="PLY05_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02583.1"
                     /translation="MMTQERFEKIQQVVQPISAVSTWLFPKTLKLFLLGLISIVWLVF
                     YSLQFWSWHYVWLLPIAMLSIPLLIMAIWSLMLWDLTDLPEALESLKNGVLGVKERVA
                     GDKQEALKVVVRVSQARKLPSMLKELWGMVEGVDAIRTVVGHALFLANPVSWGLLILA
                     VIAITLYILSASISFIFWLL"
     gene            complement(4296..>4490)
                     /gene="fadA"
                     /locus_tag="PLY05_04440"
     CDS             complement(4296..>4490)
                     /gene="fadA"
                     /locus_tag="PLY05_04440"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212712.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC02584.1"
                     /translation="ILENSDSFVNLNGGAIALGHPLGCSGSRITTTLLNVMEWKDTEF
                     GLATMCIGLGQGIATIIQRV"
     assembly_gap    4491..4590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4591..4784)
                     /gene="fadA"
                     /locus_tag="PLY05_04445"
     CDS             complement(<4591..4784)
                     /gene="fadA"
                     /locus_tag="PLY05_04445"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212712.1"
                     /note="FadA; fatty acid oxidation complex component beta;
                     functions in a heterotetramer with FadB; similar to
                     FadI2J2 complex; functions in beta-oxidation of fatty
                     acids; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC02585.1"
                     /translation="MSLNPRDVVIVDGVRSAMGRSKNGMFRHVRADTISAELVKALLV
                     RNPAVNPADIEDVIWGCVNQT"
     gene            complement(4798..6948)
                     /gene="fadB"
                     /locus_tag="PLY05_04450"
     CDS             complement(4798..6948)
                     /gene="fadB"
                     /locus_tag="PLY05_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008302483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HNC02586.1"
                     /translation="MIYSGTAISVQALEGGIAEFKFDLANESVNKFNRATLEDLKSAI
                     EAVKADSNIKGLVVTSGKPVFIVGADITEFVGSFAQGEAAIIEWVASANQIFNAFEDL
                     PFPTVAAINGIALGGGFEMCLVCDYRVMSTAAKVGLPEVKLGIFPGFGGTVRLPRVIG
                     IDNAVEWIAAGAEKRPDAALKDGAVDAVVAPDQLQAAALSLVQQCIDGKIDWKAKRAE
                     KLNPVKLNMLEQMMAFQTATAVVVGKAGPNYPAPKLALQGMQQHASLGREDALKVEAK
                     NFAKAAVTPQANALIGLFMADQAVKKASKKHEGKGSKEINQAAVLGAGIMGGGIAYQA
                     ASKGTPIIMKDIRTEALDLGMGEASKLLGKQVEKGRLTPADMGKTLSRIRPTLNYGDF
                     NSVDIIIEAVVENPKVKTAVLSEVEGLVKEDTILASNTSTISITYLAKSLKRPENFVG
                     MHFFNPVHMMPLVEVIRGEKSSDEAVAATVKLAQKMGKTPIVVNDCPGFLVNRVLFPY
                     FGGFDMLVRDGADFQAVDKVMEKFGWPMGPAYLLDVVGIDTGVHAAKIMAEGFPDRMQ
                     PNYKSGTEIMFENNRYGQKNGKGYYVYEQDKKGKPKKVVDQTTYELLAPIVAERKEFD
                     AQEIIDRCMIPMVNEVARCLEEGIVASAAEADMALIMGIGFPPFRGGACRYVDQVGIA
                     NYVAACEKYAHLGKAYEAPKLLLDMAAANKKFFG"
BASE COUNT         2096 a         1603 c         1184 g         2005 t
ORIGIN      
        1 ctttattagg gtcagcataa acattacgca cactggcctc aaccatattg cgtggcaagg
       61 tgtattccat cattttgttt aacacttgct atgtatgtaa actagcggat gtaccatgta
      121 acaacacaat gggcacaggg tcggatttta agccttcatc acgaatatgc acttgttgac
      181 catcaaccgt cataaattgc gatggcgcaa ctgcccaacg tggacttaat tccgccactg
      241 ttttatctgg cacatataaa gcacctgcac ttaataacag taccaccaac actattacag
      301 cggctatttt ggctttgctg agtttcattt tttatattca cttatgtcgt taaaatcaaa
      361 agcagtaaac atgccttttt ttaggccaga aattgttatt gccatttctt tacgaaaata
      421 aggcacacga tacatccaaa taaaagcaaa gtctcttaat ttggctgccc attcattatc
      481 tgattgatag agtggggtta gttgtcggct taaccactga taaaaatggg tcgatgaggc
      541 tcttaattgg tgataatttt gccataactc atttagatct gtggctttgg cgcaagcttg
      601 ccctaatgcc cacgcatcta ataaagccat gtttgcacct tgccctaatt gtgggctcat
      661 tgcatgagct gcatcaccaa tgacaccaat acgtccttga ccaaaacgtg tcatcaccac
      721 atctcgataa ttggcggcta accattgatc tggttgatta atcaccgtcg ctaaccactg
      781 cgccaccaca ggccaacgtt gatcgacttg tgttaaccaa tcttgtcgtt gcttttcggt
      841 ttttccccat tgctgaagct gttgactgtg taaactccaa aacacactcg ataaacgttg
      901 tgttggtgca tcaggcattg cacctgttgg caaaacaccc atcataattt ttgcaccgtc
      961 ataaaactga tgcaaaatct gtgtatctaa tgctaaacac tctggcacaa tcgtccatgc
     1021 tgcgccccaa ggatatgatt tatctaacct gacccactct ttaggccgca actgacttcg
     1081 tgcaccattc gcgacaatca ctaaatcgaa attggtggta aaagcttgtt gctgtatgct
     1141 gcctgtcacc acacaactgc cttgttgctc ttccacttta tcaatgtgcg cgcccatatg
     1201 ccacgtgatg tgatgggtga agttttgatt ttgcaaagca gtcattaaca catgacacaa
     1261 ggttgcccga tgaattccta agccataaag attttgccct gcttgcagat agtgactatt
     1321 gactaagagt tgttgattgg gcaatcgacc ttctaagccc gttacaacag cccccaaacc
     1381 taaagcatgt tctaaaacgc ccaaatgagc aaagactgcc aaacctgcgg gctgtaataa
     1441 caaacctgca cccacaggcg ataaatgatc aaccgcttca aaaatggtaa ttttgtgtcc
     1501 ttgacgggca agaataatgg cggctgcaag acctgctgtg cctgcaccaa taatggcaat
     1561 atgctgttcg tgcatccgta aattttttcc tagattttat aaacgtggtc gtcctcttgt
     1621 cttatacgct caaactcagc agcaacacgc gcagcaaaca ctttaaatac cgcagatata
     1681 agtgctgtat ctaataaagg acgtctaaac aataacacta tttggcctgt atattcgcct
     1741 tgtgaatttt ctattcggcg accaatataa gactccgcac caaacattct taaaccttct
     1801 gctaagggat attgttcgat aacattacaa gacacaatcc actctttatg gattgtagtt
     1861 aatcgctcac aaggtgttcc aataattgga atattgatat tatccaataa attaccatct
     1921 acaaccaacg caacactgcg tgttaatgat ggtttatcca attgtactct agaaataaaa
     1981 ccaacttcag cccccaagat tttggtcact gctaaggtca atttttgaaa aaaatgatcg
     2041 cctgtagcta aagaaacaac atcggcaatt tgataaatag ttgctaaact ttgctcattt
     2101 tctttacgcg ctcttaaatt tctaatgcca taagaaatgc tatctgccaa tttttctaac
     2161 aacaatactt cttcttgttc tatatcaaat acttcgtggg aaacaatcga caatacacca
     2221 aaagcattgc ctaaatgatc ttttaaggct aaagcaatac ctccacgaat acctcgactc
     2281 gttgccatct gttgtactgc taacgattca acttggagaa aatcttgaca taaaatggtt
     2341 ttagattgac gaatacatgg gcctgttaga ccctgaccac ggatatcgtc cgcagaccac
     2401 gacagataat ccttaaaatc agcggcttct atccccacat aggctacagg caaaattgtt
     2461 tttcgttcat cttgttgagc agaaccaatc cacacatgtg gatagccacc acgatccata
     2521 attaattgac aaatattttg caataaacgt tgttcttcat cgacttgtaa cagcaaagcc
     2581 tgacattcgg tgatcattga taaagagcgt tctgccctac gccgctcagt cacatcatga
     2641 cctgaaggca ctaaataaga tacattattc ttaacatcaa aaactggatg aagactaaaa
     2701 tcaataactc tcaacttgtg attaatatct ctaaatacca tatcaaaacg agaagattga
     2761 ccttgtaatg ccttataaat tgccgaacgt aattgctctc tttgtgtttc ggaatgcgcc
     2821 caccaagggg tctcatcaaa tttacgcccc aaaacgtctt tacgttctat gcctacagca
     2881 tctaatgccg tctgattagc ataggtaaca cacccttcta aatttaacat accgataaaa
     2941 aaagcgggac ttaaaccatc catgacttct cgtaaacgtt ttgaagcctt atcgactttt
     3001 tctaattcat tatgataata accagctagg ggcatttttc tctcctagaa caaatcctac
     3061 taataatcca gtaaaacatt aggattactg tttatttgtt ttagtcttaa attaaagcat
     3121 ttattgaagg cttgatgtga acataaccca agcatttatt tttcaatgta aagcaaatca
     3181 ttatataaaa aaacataata aaaataatat atatatcagt ttaacaacat catcatattt
     3241 atatattagt catccctcgt caatacttct aacaactcta tctcaaagat taaatttgaa
     3301 tttggttgaa tgtatgcgcc aatttttcgc tctccataag ccaaatgcgc aggcacaaat
     3361 aatttgcgct tgccgcccac tttcataccc attaaaccaa tatcccaacc tttaatcact
     3421 cgacctgtgc caatcacaca ttgaaaagct tggccacgat cataagaaga gtcaaatttt
     3481 gtaccatctt ctaaccaacc agtatactga gtcgttatta atgcaccttt aacaacggct
     3541 ttgccttcgc caagcacaat atctgttatt tcaatttgtt cagacattta tttttcctac
     3601 aacagccaaa atataaaact aatactagca gagagaatat ataaagtaat agcaataacg
     3661 gccaagatta ataatcccca actgacagga ttagccaaaa acaacgcatg gccaacaact
     3721 gttctaattg catctacccc ctcaaccatg ccccataact ccttgagcat ggatggcaat
     3781 ttacgcgctt ggcttactct gaccacgact ttgagtgctt cttgtttatc accagccaca
     3841 cgctctttta cacctaatac accatttttt aggctttcta aggcttctgg taaatctgtt
     3901 agatcccata acattagaga ccatatcgcc ataatcaaca aagggattga taacatggcg
     3961 atgggcaata accacacata atgccatgac caaaattgca aactataaaa caccaaccac
     4021 acaatagaaa ttaatcccaa taagaataat ttgagtgttt tgggaaatag ccatgtgcta
     4081 actgcactaa tgggttgtac aacttgttga attttttcaa atctttcttg tgtcatcatt
     4141 agcaatatta tccttttagt gctgaaattg ctcagcaact tagcaaattt caccaaaaat
     4201 taaaaacccc caaaccttgt gagtctgagg gttagtattg tcttggataa agactaattt
     4261 tttagggcaa ccactaaagc cacccctaca caacattaca cgcgttggat aatggttgcg
     4321 ataccttgac ctaaaccaat acacatggtc gctaagccaa attcagtatc tttccattcc
     4381 atgacgttta acaatgttgt agtaatacga gaaccagaac aacccaatgg gtgacctaaa
     4441 gcaatcgcac caccatttaa gttaacaaag ctatcagagt tttcgaggat nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gtttggttaa cacaacccca aatcacatct
     4621 tcgatgtcgg ctggattaac ggctgggtta cgcactaata aagcctttac taattcggca
     4681 gaaatggtat cggcacgcac atgacggaac ataccgtttt tggagcgacc cattgcacta
     4741 cgtacaccat caacgatgac tacatcacga ggattaagac tcatttttcg ctcctaatta
     4801 accaaaaaat ttcttgttag cggcagccat gtcaagcaac aattttggtg cttcatatgc
     4861 cttaccaagg tgcgcgtatt tttcacaagc agcgacataa ttagcaatgc ctacttggtc
     4921 aacatagcga catgcaccgc cacggaatgg tgggaaaccg atacccataa ttaaggccat
     4981 atcagcttct gctgcactag caacaatacc ttcttccaaa caacgagcca cttcgttaac
     5041 cattggaatc atacaacgat caatgatttc ttgagcgtca aactctttac gttcagcaac
     5101 aataggtgct aacaactcat aagttgtttg gtcaaccact ttctttggct tgcctttttt
     5161 gtcttgttcg taaacataat aacctttgcc attcttttgg ccataacggt tattttcgaa
     5221 catgatttct gtgccagact tgtagttagg ttgcatacga tctgggaaac cttcagccat
     5281 aattttggct gcatgaacgc ccgtatcaat accaacaacg tctaacaagt aggctggacc
     5341 cattggccaa ccgaattttt ccatcacttt atcaacagct tggaagtcag caccatcacg
     5401 gactaacata tcaaagccac cgaagtatgg gaataatacg cggtttacca agaagcctgg
     5461 gcaatcgtta acaacgatag gtgttttacc cattttttgt gctaacttaa cagttgcagc
     5521 aaccgcttca tcagaagact tttcaccacg aataacttca accaatggca tcatgtgtac
     5581 tgggttgaag aaatgcatac cgacaaagtt ttctggacgc tttaaagact tcgctaagta
     5641 agtaatagaa atagtagaag tattagacgc taaaatagtg tcttctttta ctaaaccttc
     5701 aacttcgctt aatacggctg ttttaacttt tggattttca acaaccgctt cgataatgat
     5761 atcaacgcta ttaaaatcac cgtagtttaa ggttgggcga atacgagata aagttttacc
     5821 catatcggca ggagttaaac ggcctttttc gacttgctta cctaataatt tagaggcttc
     5881 gcccataccc aaatcaaggg cttcggtgcg aatgtccttc ataatgatgg gtgtgccttt
     5941 gctggctgct tggtaggcaa taccgccacc catgatgcct gcacccaata ctgcagcttg
     6001 attaatctct ttgctgcctt tgccttcatg ctttttagaa gctttcttaa ccgcttggtc
     6061 agccataaac aaaccaatta aggcattagc ttgtggagta accgctgctt tagcaaagtt
     6121 tttagcttct acttttaagg catcttcacg gcctaagctg gcgtgttgtt gcataccttg
     6181 taaagctaat tttggagctg ggtagttagg accagcttta ccaacaacaa cagccgttgc
     6241 agtttggaaa gccatcattt gttctaacat gttgagttta actgggttta atttttcagc
     6301 gcgttttgct ttccagtcaa ttttaccgtc gatacattgt tgtaccaaag ataatgcagc
     6361 agcttgtaat tggtctggtg caacaacagc atcaacagca ccatctttta aagcagcatc
     6421 tggacgcttt tctgcaccag cagcaatcca ctcaacagca ttgtcgatac caatcacgcg
     6481 aggtaaacgt actgtgccac caaagcctgg gaaaatacct aatttaactt ctggtaaacc
     6541 cacttttgca gcggtggaca tcacacgata gtcacacact aagcacattt caaaaccgcc
     6601 acctaaagcg ataccattaa tcgcggcaac agttgggaat ggtaaatctt caaaagcgtt
     6661 gaaaatttgg tttgcagaag caacccattc aataatcgcc gcttcgcctt gagcaaaact
     6721 accaacaaac tcagtaatgt ctgcgccaac gataaagaca ggcttaccgc tagtcacaac
     6781 taaaccttta atgttgctgt ccgctttaac ggcttcaatc gcacttttca agtcttcgag
     6841 cgtggcacgg ttaaatttgt taaccgattc attggcgaga tcgaacttaa actcagcaat
     6901 accgccttct aaggcttgta cactgatggc agttcctgaa taaatcatgc ctaaatctcc
     6961 atcgtggtgg tttaaactca gtattaat
//
LOCUS       DAOQMB010000081         6919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4559_length_6919_cov_15.122232, whole genome shotgun sequence.
ACCESSION   DAOQMB010000081 DAOQMB010000000
VERSION     DAOQMB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_6919_cov_15.122232"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="PLY05_04455"
     CDS             complement(<1..1319)
                     /locus_tag="PLY05_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02587.1"
                     /translation="MKNQLTLLTASLLLLQNAYAENNKMLSAASQFNPSISLILDGNY
                     YHDNKKGKASDYLEEIAGISHTVHDTHAEHEHGLEKGFNLGESELVMSAIVDPYFDGK
                     LTLAIDSQGATELEEAWLQTRMLPAGLKVKMGKFLSDIGYLNNQHPHTWDFADQNLAY
                     SALLGEHGLMDTGLQLTWLAPTPFYALFGLEALQGKEQERFGALVDEELNHDTIDENN
                     MLGSFAEQKAGPRMNTLFAKFAPDLGSNHALQWGFSYAQATQYQQLLDEDETAQTGDE
                     MALDGKQTLIGTDIVYKWDGTGQYGQGDFKVIAEYLTLKKDMSVVASGVGIDTDNDGN
                     LDELPLAIGSKVTGKQDGYTLQASYGIAPRWQVSLRHDATGNTNELNNAGTKVSFKDS
                     SRNSLAVSFYASEFSRLRLQASQADLADETGKVEKVNQVFLQYTHSLG"
     assembly_gap    1320..1419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1420..1836
                     /locus_tag="PLY05_04460"
     CDS             <1420..1836
                     /locus_tag="PLY05_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02588.1"
                     /translation="CRTLARKRALVSAGVAVVPVPFLDVMVDASMLTVLIPEISRRFG
                     LAPEQIEAFDAETKKVVWSEVAKRGSQFIGLVVTRTLVRQSVQNMATKVISKQVAKFI
                     PFGGQMVAAGLGYFVLRRIVYQHIDDCYEVAKASFN"
     gene            complement(1829..4630)
                     /locus_tag="PLY05_04465"
     CDS             complement(1829..4630)
                     /locus_tag="PLY05_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC02589.1"
                     /translation="MSIINGMFKKLSFLLPRNFTRQMAVFSAISLCVALAIFAGTIYQ
                     QQRTINQKSAVNQGILVIKNLSTIVEQDVLIANKKSIRTLLLNTAIFSEIRELIITDN
                     EGTVLVHVEHKLNEEPTEKANNSQLKTPKTKDLYYTVDKENSQIFFWQPIADYGWAYM
                     LYDYKNQSDTYLQILKRALLFAFIISIISGFLLFYLLKSPIQQLNRLIDFAKQLDVYK
                     KGEQSSIHIDSHLEEIQELHSALNQSALRLYQQSQELIQSKERYSHVINNVHEVIFQT
                     NTQTMFSFLNEAWQKLSKYPIENSLGKPMVDFLSEQHQEHIFHALQKLFNGSISEFRD
                     NVQIITAAHEEIWVDLWISLIYEKDKNGNTIICGTTGTINDISTQKQTESALIAAKEA
                     AESATKAKSEFLATMSHEIRTPMNGILGMAQLLLETPLNNEQQEYSRILYHSGQALLT
                     IINDILDFSKIEAGKLTIEMIPFDLQKICEEVCDLLLSQVQSKNIELVLAYSPSCPRT
                     LIGDPNRIRQILLNLLGNAIKFTKNGYVALKVYADVITPATVDLNIEIIDTGIGIPLD
                     KQSKLFQLFSQADATTTRRFGGTGLGLAISKGLSELMGGTITLESTPSIGSTFKVQLS
                     LKRQHAVERSMPQYPELYFMPALVVDSLEINRKHISDCLQRYGMRVETASSAEEGLVK
                     LKLMNHLSPRVPFILIDQNLTKSSEGLSFAHSIRQNPIFSNSHLILMSQTPLRNLPNS
                     LYAAFNGFIAKPLRFYQLINEISALNSGKKTFLQTKENLAETSETNANLSLNNTLNTP
                     IINSTSAINRGAIRALLAEDNIVNQKVALKMLEKIGIDVDIANNGKEAVKMAEEKDYD
                     LIFMDCQMPEMDGFDATRSIRHLEQKNAKEATPIIALTANALEGDKNQCLAAGMTDFL
                     AKPLKQDTLHNLLKKYRFN"
     gene            complement(4632..>4871)
                     /locus_tag="PLY05_04470"
     CDS             complement(4632..>4871)
                     /locus_tag="PLY05_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02590.1"
                     /translation="QSLLADLGYNNKQFKELLKQQAWHGMIEHNTNQWLQLDLPELPS
                     FYLQGEKRISVCGAHRLWALEMALIEQFNNQNPLK"
     assembly_gap    4872..4971
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4972..5276)
                     /locus_tag="PLY05_04475"
     CDS             complement(<4972..5276)
                     /locus_tag="PLY05_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02591.1"
                     /translation="MPFGQRLLNPILTRTLRHPVMQELFHHRHEWWRQLRGKPQQVVV
                     WLRFTDPYSYLLIQTLPNFISHFDVKLIFKLLPYQEPNQFFSYYLRDAWHLAQFHQL"
     gene            5424..5978
                     /locus_tag="PLY05_04480"
     CDS             5424..5978
                     /locus_tag="PLY05_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNC02592.1"
                     /translation="MKQELPLFPLNTVLYPQGELPLRVFELRYRRLLDECGKTQPFGV
                     VRIRYGQEVGEPAFPYDVGCAAYVVQQVALEDGSLAALAVGDRRFKILSINIEEDGLA
                     RAQIEWLKPEPIVAIPDDLSEIAEAFTECGDRLSDAGSLAWRLAEALPLSLDEQQQLL
                     EEENPRQRLQLVKKLTLKYPIDFI"
     gene            6092..6724
                     /locus_tag="PLY05_04485"
     CDS             6092..6724
                     /locus_tag="PLY05_04485"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02593.1"
                     /translation="MKKENIIKEHYHHGSLRDALISAAVQRLQSDGIAKLSLRALAAD
                     VGVSPTAVYRHFEDKSALLAAIACDGFVGLTQNMHKYLGHEPCDALVALKRIGIGYVD
                     YAIHHPAHYRLMFGQRMVERARYPDLFQASNTSFAMLRNTIKRGIDEQLFRDLPLEVM
                     TMTAWSLVHGMAMLTIDGLVKHPNQTDALMTIANQVQDTLAHGLLRRPSL"
BASE COUNT         2007 a         1168 c         1320 g         2224 t
ORIGIN      
        1 cctaagctat gggtatattg caaaaatacc tgatttactt tttcaacttt gcctgtttca
       61 tccgctaaat cagcttggct cgcttgtaag cgtaagcgcg aaaactcact ggcataaaag
      121 ctcacagcaa ggctattacg gctagagtct ttaaagctga ctttagtgcc tgcattattt
      181 aactcattgg tattgcctgt ggcatcatgg cgtagtgata cttgccaacg cggtgcgata
      241 ccataactgg cttgtagggt ataaccatct tgtttgccag ttactttgct accaatggct
      301 aaaggaagtt cgtctaaatt accatcatta tccgtatcta tacccacacc tgaagcaaca
      361 accgacatat cttttttaag tgtcagatat tcggcaatga ctttaaagtc accttgacca
      421 tattgaccag taccatccca tttataaaca atatctgtcc caatgagtgt ttgcttgcca
      481 tctaatgcca tttcatcgcc agtttgcgcg gtttcatctt cgtctaataa ttgttgatat
      541 tgcgtggctt gggcataaga aaaaccccat tgcaaggcat gattactgcc taaatcgggt
      601 gcaaatttgg caaaaagtgt attcatgcgt ggccctgctt tttgttcagc aaagctaccc
      661 agcatattat tttcgtcaat agtgtcatgg ttgagttctt catcaactaa tgccccaaaa
      721 cgctcttgct ctttgccttg taaggcttct aaaccaaaca gggcataaaa tggcgtgggg
      781 gcaagccatg tgagttgtaa acctgtatcc attaggccgt gttcacctaa taaggcacta
      841 taggctaaat tttggtcggc gaaatcccaa gtatgaggat gttgattatt taaataacca
      901 atgtcactta aaaacttacc catttttacc tttaagccag caggtaacat gcgtgtttgt
      961 aaccatgctt cttcaagctc agttgcgcct tgactatcaa tcgctaaggt taatttgcca
     1021 tcaaaatatg ggtcaacaat tgccgacatc accaattctg actcacccaa attaaagcct
     1081 ttttccaaac catgctcgtg ttctgcatgt gtatcatgta cggtgtggct aattcctgca
     1141 atttcttcta aataatcact ggctttgcct tttttattat catgataata attaccatct
     1201 aaaattaatg agatagaggg attaaattgt gaagccgcac ttaacatttt attgttttcg
     1261 gcataagcat tttgtagtaa taataagctt gcagtgagta atgtaagttg gtttttcatn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gtcgaacgtt ggcacgtaaa
     1441 cgcgcattag tatcggcagg ggtggcggtt gtaccagtgc catttttgga tgtgatggtt
     1501 gatgccagta tgttgacagt attaattcca gaaattagtc gtcgttttgg cttagcacct
     1561 gaacaaatag aagcatttga tgccgaaact aaaaaagtag tgtggtcaga agtagcaaaa
     1621 cgaggttcgc aatttattgg tttagtggta acgcgaactt tagtgcggca aagtgtacaa
     1681 aatatggcaa ccaaagtgat ttctaaacaa gtggcgaaat ttattccttt tggtgggcaa
     1741 atggtagcag caggattggg ttattttgtg ttaagaagaa tagtctatca acatattgat
     1801 gattgttatg aagtagctaa ggcgagtttt aattaaagcg atattttttt aataaattat
     1861 gtaaagtatc ttgtttgagc ggtttagcca aaaaatcggt cattcctgcg gctaaacatt
     1921 ggtttttatc gccctccaga gcgtttgcgg taagagcaat aatgggagtg gcttctttgg
     1981 catttttttg ctctaggtgt ctaatactac gagttgcatc aaaaccatcc atttcgggca
     2041 tttgacaatc cataaaaatt aaatcgtagt ctttttcttc ggccattttg actgcttctt
     2101 tgccattatt agcaatatct acatcaatgc caattttttc gagcattttt agggcaacct
     2161 tttgatttac aatattatcc tcagctaata aggctctaat tgcgccacga ttaattgctg
     2221 atgtgctatt aataattggt gtatttaatg tattgttcaa tgataaatta gcattagttt
     2281 ctgacgtttc ggctaagttt tctttggttt gtaaaaaggt ttttttacca ctattgagcg
     2341 cggagatttc gttaattaat tgataaaagc gcaaaggttt ggcaataaag ccattaaatg
     2401 cagcatataa actattgggt aagtttcgta atggcgtttg actcattaaa atcaaatggc
     2461 tattagaaaa aatcggattt tgacgaatag aatgtgcaaa tgataaccct tcggaagatt
     2521 tggttagatt ttgatcaata agaataaatg gcacgcgtgg cgataaatga ttcattaatt
     2581 tgagtttaac caagccttcc tcagcagagc tagcggtctc aacacgcata ccatagcgtt
     2641 gtagacagtc gctgatatgt ttgcggttga tttctaaact gtccactacc aaagcaggca
     2701 taaaatacaa ctcaggatat tgtggcatag agcgttcaac ggcatgttga cgttttaggc
     2761 taagttgtac tttaaaggtg gagcctatac taggtgtgct ttcgagagta attgtgccac
     2821 ccattaattc ggataagcct ttactaatcg caagccctaa acctgtgccg ccaaaacgtc
     2881 gtgtggtggt tgcatctgct tgagagaata attgaaataa tttgctttgt ttatcaagag
     2941 gaataccaat gcctgtatca atgatttcaa tattcaaatc aacagtcgca ggggtaataa
     3001 catcggcata aacttttaaa gcgacataac catttttagt aaatttaatc gcattgccta
     3061 ataaattgag caaaatttgc cgaatacgat tagggtcacc aatgagtgtt cgtgggcagc
     3121 ttggactata agcaagtact aattctatat ttttgctttg aacttgtgat aataataaat
     3181 cacagacttc ttcacaaatt ttttgtaaat caaacggaat catttcaatg gtgagtttac
     3241 cagcctcaat tttagaaaag tctaaaatgt cattaataat ggttaataag gcttggccag
     3301 agtgatataa aatacgcgaa tattcttgtt gctcattatt taagggagtt tctaataata
     3361 attgtgccat gcccaaaata ccattcatgg gggttctaat ttcatggctc atggttgcta
     3421 aaaattcact tttagcttta gtcgcagatt cggcggcttc tttggccgca attaatgcac
     3481 tttctgtttg tttttgagta ctaatatcat taatcgtgcc tgttgttcca caaattatcg
     3541 tattaccatt tttgtctttt tcataaatta aactaatcca taaatcaacc caaatttctt
     3601 catgtgctgc agtaataatt tgtacattat ctctaaactc actaatggaa ccattaaata
     3661 atttttgaag cgcatgaaaa atatgttctt gatgttgctc tgatagaaaa tcaaccatcg
     3721 gtttacccaa tgaattttca atcggatatt tacttaactt ttgccatgct tcgtttaaaa
     3781 agctaaacat agtttgcgta tttgtttgaa aaataacttc atgaacgttg ttgatgacgt
     3841 gactataacg ttctttagat tggataagtt cttgactttg ttgatataaa cgtaaggctg
     3901 attgatttaa cgcgctatgt aactcttgga tttcttctaa atgagaatca atatgtatag
     3961 agctttgttc acctttttta taaacatcca gttgtttggc aaaatcaatt aaacgattga
     4021 gttgttgaat aggacttttt aataaataaa ataacaaaaa acccgaaata atagaaataa
     4081 taaacgcaaa taataaagct ctttttaaaa tttgcaaata agtatcagat tggtttttat
     4141 agtcatagag catatatgcc caaccataat cggcgatcgg ctgccaaaag aaaatctgac
     4201 tattttcttt atcaacggta tagtataaat ctttggtttt tggtgttttt aattggctat
     4261 tattagcttt ttcagtgggc tcttcattca atttatgttc aacatgaact aaaacggtgc
     4321 cttcattgtc agtaataatt aattctcgaa tttcagagaa aattgccgta tttaataata
     4381 aagttctaat agattttttg ttggcaatta atacatcttg ttcaacaata gtactaagat
     4441 ttttgatgac caaaatacct tgattaacgg cacttttttg gttgattgtt cgttgttgtt
     4501 gataaatagt tccagcaaaa atagccaaag ctacacataa tgatattgca gaaaaaacag
     4561 ccatttgccg tgtaaaatta cgcggcaata aaaagctgag ttttttaaac ataccattaa
     4621 tgatagacat attatttgag cgggttttga ttgttaaatt gttcaatcaa tgccatttca
     4681 agtgcccata agcggtgtgc accacagaca ctgatacgct tctctccttg taaataaaaa
     4741 ctaggtaatt ctggtaggtc aagttgcaac cattggttag tattatgttc aatcatacca
     4801 tgccaagctt gttgttttag caattcttta aactgtttgt tgttataccc taaatcggct
     4861 aataatgatt gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntgtaactga
     4981 tgaaattgtg ccaaatgcca agcatcacgc aaataataac taaaaaattg atttggctct
     5041 tgataaggta ataatttaaa aatgagtttg acatcaaaat gactaataaa attgggtaag
     5101 gtttgaatca ataaataact ataaggatcg gtaaaacgta accaaaccac cacttgttgc
     5161 ggtttgccgc gtaattgtcg ccaccattca tgtcgatgat ggaacaattc ctgcatgaca
     5221 ggatggcgta aggttcgagt gaggatcggg tttagcaatc gctggccaaa aggcataagt
     5281 tgtccttaca tttttttagt tttgagctag tctaaaataa tatggggact atctcaatat
     5341 caggataact atagctgaca gaatgttttt tgtaatgata tttagattac cgcatcggtc
     5401 tttttatcaa ggtcaagtca ataatgaagc aagaattgcc actattcccg ttaaatacgg
     5461 ttttatatcc acaaggcgaa ttgccgttgc gcgtgtttga gttacgttac agacgtttat
     5521 tagatgaatg tggcaaaact caaccctttg gtgtggttag aattcgttat ggtcaagaag
     5581 taggtgagcc tgcatttcct tatgatgtgg gttgtgccgc ttatgtggtg caacaagttg
     5641 ctttagagga tggtagtttg gccgctttag ctgtcggtga cagacgtttt aaaattttga
     5701 gtattaatat tgaggaagat ggtttggctc gtgcccaaat agaatggctc aagcctgagc
     5761 ctattgtagc tattcctgat gatttatctg aaattgctga agcttttact gaatgtggag
     5821 atcgattaag tgatgcaggt agtttggcat ggcgtttggc cgaggcattg cctttatcgt
     5881 tagacgaaca acaacaatta ttagaagaag aaaatccacg ccagcgttta caattagtta
     5941 aaaaactgac attaaagtat cctattgatt ttatttgata attttattgt gtgactattc
     6001 taactatttt agtctctcat tttgtttaaa ataatgttga cagtgttaac attgttggtt
     6061 attgtaaata aaactaaagt taagtcgatt tatgaaaaaa gaaaatatta tcaaagaaca
     6121 ctatcatcac ggcagtttac gagatgcatt aattagtgct gctgtacaac gtttgcaaag
     6181 tgatggtatt gccaaattga gtttgcgggc tttagcggct gatgttggcg tgtcacctac
     6241 ggctgtgtat cgccattttg aagataaatc ggcgttgtta gcggcgattg cgtgtgatgg
     6301 ttttgtgggt ttaacgcaaa atatgcacaa gtatttaggc cacgagccgt gtgatgcctt
     6361 agtagcgtta aaacgtattg gcataggtta tgtcgattat gccattcatc atccagcgca
     6421 ttatcgtttg atgtttggcc aacgtatggt tgagcgcgcc cgttaccccg atttatttca
     6481 agcatcaaat accagttttg ccatgttacg taacaccatt aagcgtggca ttgatgagca
     6541 attatttaga gatttaccgt tggaagtcat gaccatgacc gcatggtcgt tggtgcatgg
     6601 tatggccatg ctaaccatag atggtttagt taaacatccc aatcaaacag atgcactgat
     6661 gacaattgct aatcaagtgc aagacacctt agcacatggt ttgttacgca gaccctcact
     6721 ctaactttca ccatgctgag tgatggaaac gtaaattatt ttgccttaat ttggagcaaa
     6781 cagtcatgac cgaagcagta gcgaccccac aaaaaaaatc aggtttaaca cccagtacca
     6841 ttcgtattgg caaacgctat cgagccaatc gtttaaatgg tgactatgat gccattgtta
     6901 ttggttcggg cattggcgg
//
LOCUS       DAOQMB010000082         6888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4585_length_6888_cov_16.441826, whole genome shotgun sequence.
ACCESSION   DAOQMB010000082 DAOQMB010000000
VERSION     DAOQMB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4585_length_6888_cov_16.441826"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..379)
                     /locus_tag="PLY05_04490"
     CDS             complement(98..379)
                     /locus_tag="PLY05_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4642 family protein"
                     /protein_id="HNC02594.1"
                     /translation="MALSQPTTFNEEWNDERINSYLNRQAPAGEDADFNVLYVAYKHM
                     RPSDYERFLTAFTAQGRNLQAINSQGLTIQQLISQHPSSTEFLNVLAKF"
     gene            complement(449..721)
                     /gene="minE"
                     /locus_tag="PLY05_04495"
     CDS             complement(449..721)
                     /gene="minE"
                     /locus_tag="PLY05_04495"
                     /inference="COORDINATES: protein motif:HMM:NF001422.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HNC02595.1"
                     /translation="MMDWRKIFGGDKKSNTANTAKERLMVVVAHQRGYGSAASYLPKL
                     REEILSVVRKYVQVPDNAVDIKVEQKDGLEVIEMDITLPDDKKIAL"
     gene            complement(725..>1066)
                     /locus_tag="PLY05_04500"
     CDS             complement(725..>1066)
                     /locus_tag="PLY05_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520449.1"
                     /note="ATPase; with MinC inhibits cell division by
                     blocking formation of the polar Z ring septums; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNC02596.1"
                     /translation="GLLASKTQKVEQGRGRLKEHLVLTRYNPNRVVKNEMMSVKDIED
                     ILAIPLLGVIPESTSVLTSSNSGSPVISDKNSDAGSAYDDLVARFLGEERPHRFLNPE
                     KKGLFAWLLGS"
     assembly_gap    1067..1166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..1689)
                     /gene="minD"
                     /locus_tag="PLY05_04505"
     CDS             complement(<1167..1689)
                     /gene="minD"
                     /locus_tag="PLY05_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HNC02597.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASFATGLALRGFKTVVIDFDVGLR
                     NLDLILGCERRVVFDFVNVIHNEAKLQQALIKDKTVENLYILAASQTRDKDALTQDGV
                     ERVLNELKQDFDYIVCDSPAGIERGAHLAMYFADEALVVTNPEVSSVRDSDRVLGLLA
                     SKTQKVEQGRGRLK"
     gene            complement(1904..2614)
                     /gene="minC"
                     /locus_tag="PLY05_04510"
     CDS             complement(1904..2614)
                     /gene="minC"
                     /locus_tag="PLY05_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HNC02598.1"
                     /translation="MSIDENKALRLMGRVLTLSRLRVQSSVPQAIVEELTKLTLENPM
                     LQQGLPVVIESDYELDLAWLVKVLKRTGVQVLGVAAGVLSEQAIALDLPILPPDHTKA
                     ASRGSTQQAATTSAAPKAVIEQPKLPAKMITDPVRSGQQVYAENSDLIVLAPVSSGAE
                     LIADGCIHIYGVLRGRAIAGATGNPQARIFCKKLEADLVAIAGVYMVADQMPNKLQGK
                     SVQIYLGEDGNIVIEALD"
     gene            complement(2962..3969)
                     /locus_tag="PLY05_04515"
     CDS             complement(2962..3969)
                     /locus_tag="PLY05_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02599.1"
                     /translation="MVINWHAFKEHKEAMPLLIFDIAMLALISLSLLWLLIDSLLINT
                     GIGILLSQQAPHLVLAYEKNWHPHLKLYDTLFTLFLLFELVIRWGWAIYKQTYHRWFF
                     YPFVHWYDVLACLPNLQFLRLLRLVSVFYRLHKLGILLIGTRLVRFAKKYYSIVLEEI
                     SDRIVLNVLEGIQKELKTNNPVASELREKVLNPQKTIITQWFANRISHFVDISYQKHE
                     QELAIYLNQLTYHAISHNQEWQQLKRRLPFVGGLIEQELNAVVSSLVNDMAQTILKDL
                     SHKDNAALHDLANAAFDTFTLSDEQLDVAIEKIAIDAIEIIKKQVAVQQWKLDEENEK
                     K"
     gene            complement(4020..6719)
                     /locus_tag="PLY05_04520"
     CDS             complement(4020..6719)
                     /locus_tag="PLY05_04520"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HNC02600.1"
                     /translation="MAIIETIKSWFGKNKPTPLEDKKTVVTAPPKTAAPIITINEQDV
                     ANSQSHESLLDILNNAQASFSVKELAIKRIEDQELLAQLASQHSIAKIRQQAAEKLVD
                     LTLIEKTAQALKQSDKGVYRILRQKLDNHEQQQKQFQEKQQRIDKICSDLNNLLNSAH
                     NPLFNAKVQSLAQQWQQLNQEFAIDVTNQQRFDQAYQQAQQLVQQHQAQSAKEQQAQL
                     MQQQLSEQFTSLSQSISTTEQLDELLSINQQLNQLRQQWQATIVIVSPNNELQNSVQT
                     DEGKLNQISRQLDKLSSQIPEIIQLSQQLLDNPIQQGTYQHLQKRLKNANLNDAIWQI
                     PILASVPQALKLAEQALKAQKQAQQKAKTEQAINTPTKAKVDHELDSLIQKIGELIAN
                     GQHKEAEPLLRQAQQYAKNHKLFDARLGDFIEEMQKMRDWAGFAIIPKKQDLLRQMQH
                     LAQQATEANLDALNHLDKIKALQAEWQGLGIANNEQEKNLWQQFKEVSQQAYAPCQQF
                     FDQQNAIQAENAAQRQALCQELKHYLDNLPEQVNWQGHIAILKKAREDWQKYHPVEAK
                     LHKKLQAEFTQIIKALEDKLHAEYQLQEDKKRGLIAKAENLLNHENIFEACHQAKDLQ
                     NTWKTLGSCGHNKDQQLWEEFRQHCDALFAKRHEAKAAKQAEDAQVVAQAEELLKQLH
                     TLLNTPEHTPTSEQVEPLLQQLDSQYFPKEANHELRKQINQCRRQWQDYLAKQVNSEK
                     QASLKQLSQTLDLCLAAENVVLAGQPVSVEMVLQWQENVAQIHHYFKNALQKRWQNLS
                     TLANDETEQQLREEKAQDACLLLELLLAIDSPASEKAARIAKKMALFEQQSYPKTSQD
                     AQVLIITTLNQILLIGGLNPERSQSLKQRLDAILMSAALSRML"
BASE COUNT         1935 a         1207 c         1308 g         2338 t
ORIGIN      
        1 tcaagaagct gtaagattaa agtcttacgg cttttttgtt tttaggggtt aaaaatataa
       61 agacttacga agtggttttc gtaagtcagt ttagtagtta aaatttagct aatacgttca
      121 aaaactcagt tgagctagga tgttggctaa tcagttgttg tatggttaaa ccttgtgagt
      181 tgatggcttg taaattacga ccttgtgcag taaaagccgt taaaaaacgt tcatagtcac
      241 tagggcgcat atgcttatag gcaacataca acacattaaa atctgcatct tcgccagcag
      301 gtgcttgacg atttaaatag ctattaatac gctcatcatt ccattcttca ttaaatgttg
      361 ttggttgtga taaggccata agagttctcc aaaaacaata acaaaagccg cataaagcgg
      421 ctttgtgatt tactaagctt gttagctatt ataaggcaat ttttttgtca tcgggtaaag
      481 taatatccat ttcgatgact tcaagcccat ctttttgttc aaccttaata tcgacagcat
      541 tatcgggtac ttgaacatat ttacgcacca ctgacagaat ttcttctcgt agcttgggta
      601 aataagaggc cgcgctgcca taaccgcgct gatgggcaac aacgaccatt aatcgttctt
      661 tagctgtgtt ggcagtgtta gattttttat ctccgccaaa tatcttgcgc caatccatca
      721 tgatttagct cccaagtaac caagcgaaca atcctttttt ctctgggttt aaaaagcgat
      781 gtgggcgttc ttctcctaag aagcgagcca ccaagtcatc ataagctgag cctgcatcac
      841 tatttttatc gctaataaca ggtgaaccgc tgttagaaga cgttaaaact gaggttgatt
      901 caggtattac ccctaacaac ggaattgcta aaatatcttc aatgtcttta actgacatca
      961 tttcgttttt aacaacacga ttagggttat aacgtgttaa taccaaatgt tcttttaaac
     1021 gaccgcgacc ctgttcaact ttttgagttt tacttgctaa taaaccnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnncttt taaacgaccg cgaccctgtt caactttttg
     1201 agttttactt gctaataaac ccaatacgcg atcagagtca cgcactgagg aaacctctgg
     1261 gttagtgact actaaggctt catcagcaaa atacatggct aaatgtgcgc cacgttcaat
     1321 accagcagga gagtcacaaa caatataatc aaagtcttgt ttaagttcgt ttaatacacg
     1381 ttctacaccg tcttgggtca aggcatcttt gtcacgtgtt tgggaggccg caagaatgta
     1441 caagttttct acggttttat cttttataag agcttgttgt aactttgctt cgttatgtat
     1501 gacattcaca aagtcaaaca caacacggcg ttcacaacct aaaattaagt ctaagttacg
     1561 taaaccgacg tcaaagtcaa tgacgactgt tttaaaacca cgtaacgcaa gtcctgttgc
     1621 aaaagaggca ctggttgtcg ttttgcctac accgcctttt cctgatgtta caacaataat
     1681 ctttgccaca gcgacgtttt cctctattga ttgtaattag gtttgagttt taaaagctca
     1741 attcctaagt taataataaa aattctatca agtttaacaa ataaacagtt catttgtctt
     1801 gtttattacc aatagatagc aacagcttgt taattataca aagttgtatt tacatattta
     1861 aggcatcaat ccgttaagtg ataacgacat aaatgctaaa aggttaatcc aacgcttcaa
     1921 taacaatatt gccatcttca cctaaataaa tttgtaccga tttgccctgt agtttattgg
     1981 gcatttgatc tgcaaccata tagacaccag caatggcaac aagatcagct tctagttttt
     2041 tgcaaaaaat tcgtgcttga ggattgcctg ttgctcctgc aatcgctcgc ccacgcagaa
     2101 cgccataaat atgaatgcaa ccatcagcaa ttaattctgc accagagcta acgggggcta
     2161 acacaatcaa atcgctattt tcggcatata cttgttggcc agaacgtaca ggatcggtaa
     2221 tcattttggc tggtaattta ggttgctcga taacagcttt aggggcggca ctagtagtgg
     2281 ctgcttgttg tgttgagcct cgagatgctg ctttagtgtg atcgggcgga agtatgggta
     2341 aatctaaggc gatagcttgc tcactgagta cccctgcagc cactcctaaa acctgaacgc
     2401 ctgttcgttt taagactttg actaaccaag ctaaatcaag ctcatagtca gattcgataa
     2461 caacaggtaa gccttgttgt aacatcggat tttcgagggt taatttggta agttcttcaa
     2521 caatggcttg tggaacagaa gactgtacgc gtaagcgcga aagcgttaaa actcgcccca
     2581 ttaaacgtaa tgctttgttt tcgtcgattg acataatttt aaaacactta agaaataaat
     2641 aaccaaaata gaagcagggg atttacgatg tgagaacaga tcaatatgaa aaatcatttg
     2701 agggctgacg ctgtgatcac ttattgatgt ataacactcg ctaattgcag tgaaatgcgt
     2761 aagtcctgat ctttgtgttg attgttctga ttattatcgt gtaaatccag tcgaaagatt
     2821 gttgcgcaag atactttgac tttgctcaag tagcaagagc tttgttatac ctagatacaa
     2881 ctttatcctt agtacttatg cgcttattgc ttatttgtct gcatttagat cgtttatgct
     2941 cacaaagtga gctaaatgat gctatttctt ttcattttct tcatcaagtt tccattgttg
     3001 cacagcaact tgttttttaa tgatttcaat cgcatcaatc gctatttttt cgatggcaac
     3061 atctaattgt tcatcagata gcgtaaaagt gtcaaaagca gcattagcaa gatcatgcag
     3121 agccgcatta tctttgtgtg ataagtcttt tagaatggtc tgtgccatat cattcactaa
     3181 gctactaact acagcattta attcttgctc aattaagcca ccaacaaatg gcagacgtct
     3241 ttttaattgt tgccattctt gattatgact aattgcatga taagtcaatt gattgagata
     3301 aattgctaac tcttgttcat gtttttgata agaaatatcg acaaagtgac tgattctatt
     3361 agcaaaccac tgtgtaatga tcgttttttg tggattgagg actttttcac gcagttcgct
     3421 ggcaacggga ttatttgttt ttaattcttt ttgaataccc tctaaaacat ttaacacaat
     3481 acgatcagaa atttcttcta aaacaatact gtagtacttt ttggcaaatc taactaaacg
     3541 ggtgccaatt aataaaatcc ccagtttatg aagccggtaa aatacagaga ctaaacgcaa
     3601 taaacgtaaa aattgcaaat taggtaaaca ggctaataca tcgtaccagt gaacaaaagg
     3661 ataaaaaaac cagcgatggt atgtttgttt ataaatagcc catccccaac ggatgactag
     3721 ttcaaataac aaaaataagg taaataatgt atcgtataat ttaagatgcg gatgccaatt
     3781 tttttcataa gccaatacta gatgaggagc ttgttggctt aagagaatgc caataccagt
     3841 attgatgagt aaagaatcaa tcagtaacca taataaactt aggctaatta gggcaagcat
     3901 ggcaatatca aagattaata aaggcattgc ttctttatgc tctttaaatg cgtgccagtt
     3961 gataaccata aatattccgt actaagactc tcccttttag ctcagggaga gtttttacct
     4021 tacagcattc gagatagggc tgcactcatc aaaatggcat ctaagcgttg ttttaatgat
     4081 tggctacgtt caggatttaa accaccaatc agtaaaattt gattgagtgt agtaataatt
     4141 aaaacttgag catcttgaga tgttttggga taactttgct gctcaaataa ggccattttt
     4201 ttggcaatac gtgcagcttt ttcactagca gggctatcaa ttgctaataa taattctaat
     4261 aataaacaag catcttgagc tttttcttca cgtagctgtt gctcggtttc atcattagcg
     4321 agtgtgctta gattttgcca acgcttttgt aaggcgtttt taaaataatg gtgaatttgt
     4381 gcaacattct cctgccattg taaaaccatt tcaacagaaa caggttgacc tgctaataca
     4441 acattctccg cagctaaaca caaatctagt gtttggctaa gctgtttgag gctggcttgt
     4501 ttttcactat taacttgctt agccaagtaa tcttgccatt ggcggcggca ttgattgatt
     4561 tgtttgcgta actcgtgatt tgcttctttg ggaaaatact gactgtctaa ttgttgaagt
     4621 aagggttcta cttgctcaga tgttggtgtg tgttctggcg tattaagtag ggtatgaagt
     4681 tgtttgagca actcttctgc ttgtgcaaca acttgtgcat cttctgcttg ttttgcagct
     4741 ttggcttcat gacgttttgc aaacaatgcg tcacaatgct gacgaaattc ttcccataat
     4801 tgttgatctt tgttatgacc acaagaacct aaggttttcc atgtgttttg taaatctttg
     4861 gcttgatggc aggcttcaaa aatattttca tgatttaata agttttcagc cttagcaatt
     4921 aaaccgcgtt ttttatcttc ttgaagttga tattctgcat gaagtttatc ttctaatgct
     4981 ttgataattt gagtaaattc agcttgaagt tttttgtgta actttgcttc aacaggatga
     5041 tatttttgcc agtcttcacg ggcttttttg agaatagcaa tatgaccttg ccagttaact
     5101 tgttcaggta agttatctaa ataatgtttt aattcttgac ataaagcttg gcgttgtgca
     5161 gcgttttcgg cttgaatggc attttgctga tcaaaaaact gttggcaagg agcataagct
     5221 tgttgagaga cttctttaaa ttgttgccac aagttttttt cttgttcatt gttggcaata
     5281 cccaaacctt gccattcagc ttgcaaagcc tttattttat ctaagtgatt gagtgcgtct
     5341 aagttggctt cagtggcttg ttgtgccaaa tgttgcattt gtcgcaataa atcttgtttt
     5401 ttaggaataa ttgcaaaacc tgcccaatca cgcatttttt gcatttcttc aataaaatca
     5461 cccaagcgtg catcaaagag tttgtgattt ttggcatatt gttgtgcttg acgcaacaaa
     5521 ggctctgctt ctttatgttg gccattagct attaattcac cgattttttg aatcaatgag
     5581 tctaattcgt ggtcaacttt tgctttagtt ggcgtgttga tagcttgttc tgttttggct
     5641 ttttgttgcg cttgcttttg tgcttttaat gcttgttcag caagtttgag ggcttgaggc
     5701 acgctcgcta ggataggaat ttgccaaata gcatcgttga gattggcatt ttttaagcgt
     5761 ttttgtaaat gttgataagt gccttgttga atagggttat ctaataattg ttggcttaat
     5821 tgaataattt ctggaatttg actagaaagt ttatccaatt ggcggctaat ttggtttagt
     5881 ttgccttcat cagtttgtac cgaattttgc aattcattat ttgggctaac aataacaatt
     5941 gtggcttgcc attgttgtcg taattgattt agttgttggt taatgcttaa taattcatcc
     6001 aactgttcag tggtggatat agactggctc agggaagtaa attgttcact caactgttgt
     6061 tgcattaatt gtgcttgttg ttctttggca gattgagctt gatgttgttg aacgagttgt
     6121 tgagcctgtt gataagcttg atcaaaacgt tgttggtttg tgacgtcaat cgcgaattct
     6181 tgattaagtt gctgccattg ctgtgccaag ctttgcactt tggcattaaa aagtgggtta
     6241 tgtgcactgt taagtaaatt gtttaaatca ctgcaaattt tatcaatacg ttgttgtttt
     6301 tcttgaaatt gtttttgttg ttgctcatgg ttatctagtt tttggcgtaa aattcgatat
     6361 acacctttat cactttgttt taaagcttga gctgtttttt caattaaagt taagtctact
     6421 aatttttcag cagcttgttg acgaattttg gcaatactat gttggctggc gagttgtgct
     6481 aataattctt gatcttcaat gcgtttaatg gctaattctt taacactaaa acttgcttgt
     6541 gcgttattta aaatatctaa taaagactca tgagattggc tattggcaac atcttgttca
     6601 ttaatagtga ttatcggtgc agcagttttt ggtggtgctg tgacgactgt ttttttgtct
     6661 tcaagggggg ttggtttatt tttgccaaac catgatttaa tggtttcaat aatagccata
     6721 tcagcttcct tttatgttat ttatcagatc agcgcaagct gtatcttgac acaaagctaa
     6781 gtcgagactt agtacaaatg atttgcagcc ttacaaaggc tatgcaacag attataaaaa
     6841 agcgaacaat tctagcatga ttaaatgacg agctaactag ctttgagg
//
LOCUS       DAOQMB010000083         6877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4595_length_6877_cov_13.939461, whole genome shotgun sequence.
ACCESSION   DAOQMB010000083 DAOQMB010000000
VERSION     DAOQMB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6877
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_6877_cov_13.939461"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1341)
                     /gene="pgaC"
                     /locus_tag="PLY05_04525"
     CDS             complement(73..1341)
                     /gene="pgaC"
                     /locus_tag="PLY05_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HNC02601.1"
                     /translation="MFLFFKTLWHSLLSFVFYYPLFMSYLWMIGAILFYWRNERKQGS
                     YQQPTPLANTPPVSVLIPCYNEGDNAEETLTHALALDYPEFEVIAINDGSKDNTAEVL
                     DRLATQYPRLRVVHLAQNQGKAMALQAGSLLANYEILICIDGDALLDKHAAHWMVKHF
                     VEDAEVAAVTGNPRIRNRSTLLGRVQVGEFSSIVGMIKRAQRSFGRLFTVSGVITAFR
                     KSAVHQVGYWSPDMLTEDIDITWKLQRDGWDVRFEPHALVWILMPETLEGLWKQRLRW
                     AMGGAQVLIKNLDVIKSPIHNHLWPLMLELCLSLLWSYAMLIMFVLWLLSFILPLSIP
                     FVGSPLIPDEGALLLGTTCLLQFALSKWLDSGYDKGLGRNYYWMIWYPLVFWLINICS
                     TIVAFPKVILLGKGQRARWVSPDRGIKPHS"
     gene            complement(1354..3312)
                     /gene="pgaB"
                     /locus_tag="PLY05_04530"
     CDS             complement(1354..3312)
                     /gene="pgaB"
                     /locus_tag="PLY05_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HNC02602.1"
                     /translation="MLLRYLILSLVIFWTRCAFAHDLENLTILSYHEIADKKDSLVPE
                     YAVTPTNFVRQIDWLRNNGYHFISVDQYLAARKGQETLPSKAVLLTFDDGYQSVYQYA
                     FPVLKMFKIPAVIALVGNWLEQKETIDFDGKKVPRSDILSWDELKEMQQSGLIEIASH
                     SHSLHQGILANPQGNKEPAATARFYDAQQQQYENETNYQQRIRADLKQNNELLRKHFG
                     YNPRIMVWPYGRYNATTKDIAIKLGMPIGLTLDDGANTASTPLYRLRRVLVENVMSLH
                     ELHQEINYRNADLSDNDRPAKIMHVDLDNIYDPDPAQQEKNLGMLLDRIVAMGVNTVY
                     LQAFADPDGNGSADAMYFPNRHLPMRADLFNRVAWQVATRTRVKRVYAWMPLLAFDLP
                     ANEPAAQDVVVTQGKDDGHLNMGYHRLSLFSPRVQQVIKDIYADLAKSSTFDGLLFHD
                     DVTFSDYEDASPWAMKTYQQWGLPTDLNQIRDNDDLLGRWTILKINALDNFAMEVANI
                     VRKEQPDLRTARNLYARVVLNPRSEVWYAQSLDNSLANYDFTAIMAMPYMENETDIEG
                     FYKTLVDVVKSKPNGLKKTIFELQATDWRDNHLIPSEELATTIKTLYDLGAIHVGYYP
                     DSIQQQHPNPSTLKAVFGTKANIPIMNP"
     gene            complement(3512..6340)
                     /gene="uvrA"
                     /locus_tag="PLY05_04535"
     CDS             complement(3512..6340)
                     /gene="uvrA"
                     /locus_tag="PLY05_04535"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HNC02603.1"
                     /translation="MNNFIRIRGARTHNLKNISLDIPRDKLVVITGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLALMQKPDVDSIDGLSPAIAIEQKSTSHNPRSTVGTI
                     TEIYDYLRLLYARVGTPYCSEHNLPLSAQTISQMVDNVLAIAEGTALMLLAPVVRDRK
                     GEHLHILESLRAQGFVRARINGIVVDLDDAPALEKTKKHTIEVVIDRFKVRADLALRL
                     AESFETALRLADGVAMISYMDNDGEDLIFSSKHSCPHCGYAVSELEPRLFSFNNPAGA
                     CPSCDGLGHKAHFAAEQLILHPELSLNEGVVRGWDKQHPYYFLLLSSVAKHYQVDMDQ
                     AWQDLPAAIQTIFLQGSKKEKISFAYTDERGNKRLREQPFEGILPHLERRYKETESPA
                     VREELSQYLITSRCDQCDGSRLRKEARYVYVADKNLPSITQLPIKKAADYFENLALTG
                     QKAEIADKVLKEIRDRLHFLVSVGLDYLSLERSADTLSGGEAQRIRLASQIGAGLMGV
                     MYVLDEPSIGLHQRDNERLLNTLLRLRDLGNTVLVVEHDEDAIRAADHIIDIGPMAGV
                     HGGQIIAQGTAEDIKQIPESLTGQYLSGKAKIAVPSQRTAINPEKILSLTGATGNNLK
                     NVSIDIPLGLMTCVTGVSGSGKSTLINGTLFPITAKALNGALSLNPAPYKSITGLEHL
                     DKVVDIDQSPIGRTPRSNPATYTGLFTPIRELFAATQEARSRGYSVGRFSFNVKGGRC
                     EACEGDGVIKVAMHFLPDVYVPCDVCHGERYNRETLEIRYKGKNISQVLNMTVEDAFA
                     FFESVPSLARKLQTLMAVGLSYITLGQAATTLSGGEAQRVKLARELAKRDTGKTLYIL
                     DEPTTGLHFHDIAQLLAVLNQLRDHGNTIVIIEHNLDVIKTADWLIDMGYEGGDGGGE
                     VVAIGTPEQVAADEKSHTGRFLKPLLSV"
     gene            6512..>6877
                     /locus_tag="PLY05_04540"
     CDS             6512..>6877
                     /locus_tag="PLY05_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005095117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC02604.1"
                     /translation="MTQGERKATSILASIFALRMLGLFMIIPVFALYGQKLEGATPAL
                     IGLAIGIYGLTQAVLQIPVGLWADRINRKLLIIIGLILFAIGGAVAAMSHSIWGVILG
                     RAIQGAGAISAVVMALLADL"
BASE COUNT         2121 a         1414 c         1375 g         1967 t
ORIGIN      
        1 atgcgagata aatatatcaa gcacttaaaa gctgtcctta tcaaatctta ttaccctaat
       61 cttaataaat tattaagagt gaggtttaat accacgatca gggctaaccc atcgtgcgcg
      121 ttgtccttta cctagcaaaa tgactttggg aaaagcaacg atggttgagc aaatattgat
      181 taaccaaaat accaatggat accaaatcat ccaataataa ttgcgaccta aacccttgtc
      241 ataaccacta tctagccatt tgctaagtgc gaattgtagc agacaggtag tccctaataa
      301 cagagcgccc tcatcaggaa tgagtggtga acctacaaaa ggtatgctta atggtaatat
      361 aaaactgagt aaccacaaaa caaacataat aagcatggca tacgaccaca acaagcttaa
      421 acaaagttct agcatcaatg gccataaatg attatggata gggcttttga taacatctaa
      481 atttttaatt aatacctgtg cgccacccat tgcccaacgt aaacgttgtt tccataaacc
      541 ttctagggtt tcaggcatta aaatccatac taaagcatga ggctcaaagc gaacatccca
      601 accatcacgt tgtagtttcc atgtaatatc aatatcttcc gtaagcatat caggactcca
      661 atagcccact tgatgaacgg cagatttacg gaacgcggtg atgacacctg acacggtaaa
      721 tagacgacca aaactacgtt gtgctcgttt aatcatgcca acaatcgatg aaaattcacc
      781 aacttgtaca cgccctaata aggttgaacg attacgaata cgcggattac ctgtaacagc
      841 agcgacttcg gcatcttcaa caaaatgctt caccatccaa tgtgccgcat gtttatcaag
      901 gagggcatca ccatcaatac aaattaaaat ttcataattg gctaataagc tacctgcttg
      961 taaagccatc gccttgcctt ggttttgtgc taaatgcacc acccgtaatc gggggtattg
     1021 tgtggctaaa cgatctaaaa cttcagcagt gttgtcttta ctgccatcat taatcgcgat
     1081 cacctcaaac tcaggataat ctaaagccaa agcatgggtg agtgtttctt cggcgttgtc
     1141 accctcgtta taacaaggaa tcaacacact cacaggtggc gtattggcaa gaggagtggg
     1201 ctgttgataa gaaccttgtt tacgttcatt gcgccaataa aacagaattg caccaatcat
     1261 ccataaatag gacataaata aagggtaata aaagacgaag cttaataaac tgtgccataa
     1321 ggttttaaaa aacaagaaca taaacccctc caattatgga ttcataatcg gtatatttgc
     1381 tttagtacca aacacagctt ttaaggtgct tggattgggg tgttgctgtt gaatactatc
     1441 gggataatag ccaacatgaa ttgcacccaa gtcatagaga gttttaatgg ttgtcgctaa
     1501 ttcttcactg ggaattaaat gattgtcacg ccaatcagtt gcctgtagct caaaaattgt
     1561 tttctttaag ccattgggtt tacttttaac aacatcaact aaggttttat aaaagccttc
     1621 aatatctgtt tcattttcca tgtagggcat ggccataatc gcggtaaaat cataatttgc
     1681 taaagagtta tcaagggatt gtgcatacca gacttctgag cgaggattta agacaacccg
     1741 agcatataaa tttcgagcgg tacgtaagtc aggttgctct ttgcgaacaa tattagcaac
     1801 ttccatggca aagttatcta aggcattaat ttttaaaatc gtccaacgcc ccaataagtc
     1861 atcattatcg cggatttgat tcaggtcagt cggtaagccc cattgctgat aggttttcat
     1921 tgcccaaggg ctagcatctt cataatccga aaaggtcaca tcatcatgaa acagcaaacc
     1981 atcaaaagtt gatgacttgg ctaaatcggc ataaatgtct ttaatgactt gttgtacacg
     2041 aggtgaaaat aacgataaac ggtgatagcc catatttaaa tggccatcat ctttgccttg
     2101 tgtaaccaca acatcttgag cggctggctc gttagcgggt aagtcaaaag caagcaatgg
     2161 catccacgca taaactcgtt tgacacgtgt gcgagtagcc acttgccatg caacacggtt
     2221 aaataaatcg gcacgcatcg gtaaatgacg attaggaaaa tacattgcat ccgccgagcc
     2281 gttaccatca gggtcagcaa atgcttgtaa atagacggta ttgacgccca ttgcgacaat
     2341 acgatcaagc agcataccta agtttttttc ttgttgagcg gggtcaggat cataaatgtt
     2401 gtctaaatca acatgcataa ttttggcggg tcgatcgtta tcacttaaat cagcattacg
     2461 gtagttaatt tcttggtgta gttcatgtaa agacatgacg ttttcgacta aaacgcgtcg
     2521 taaacgatat aaaggagtag aagctgtatt tgcgccatcg tctaaggtta aaccaatcgg
     2581 catgcctaat ttaatggcaa tatctttggt agttgcatta tagcgtccat aaggccaaac
     2641 cataatgcgt ggattatagc caaaatgttt gcgcaataat tcattgtttt gttttaaatc
     2701 agcacgaata cgttgttgat agttggtttc gttttcatat tgctgttgtt gtgcatcata
     2761 aaaacgagcg gttgccgcag gctctttatt gccttgagga ttggctaaaa tgccttgatg
     2821 tagagagtga ctgtggctag caatttctat taagccactt tgttgcattt cttttagttc
     2881 atcccatgat aaaatatcac tacgtggaac tttttttcca tcaaaatcaa tagtttcttt
     2941 ttgttcaagc cagttgccta cgagggcaat taccgcaggg attttgaaca tttttagcac
     3001 aggaaaggca tattgataaa cagactggta accatcatca aaggtgagta aaacagcttt
     3061 gctgggcagg gtttcttggc ctttacgggc ggcaagatat tgatcgacac taataaagtg
     3121 atagccatta ttgcgtaacc aatcaatttg gcgcacaaaa ttagtgggag tgaccgcata
     3181 ttctggcact aaagaatctt ttttatcagc aatttcgtga taactgagaa tagttaaatt
     3241 ctctaaatca tgggcgaacg cacaacgcgt ccaaaatata actaaactaa gaatgagata
     3301 acgtaataac atgatggtct agataataat aacagggtat taggttaaac aatgcaccaa
     3361 atctttgatg attaatgtac aaaattggtt gttatattat aacataatgt ttgtgcttgt
     3421 gtctaaacta aacaaaattt gtcatttgta ctaaaatgtc tgcttggtga gagagggtgt
     3481 gctatttcaa gagaaatagc acatgagtga atcaaacgga tagcaaaggc ttcaaaaacc
     3541 gccctgtatg tgatttttca tcagcagcga cttgttctgg tgtgccaatg gcgactactt
     3601 caccaccacc atcaccccct tcataaccca tatcaatcag ccagtcagcg gttttaatca
     3661 catctaagtt gtgttcaata atcacaatgg tattaccgtg atcacgcaac tgattgagaa
     3721 cagcaaggag ttgagcaata tcatggaagt gtaaaccagt tgttggctca tccaaaatat
     3781 acaaggtttt gcctgtatca cgttttgcta attcacgcgc cagttttacc cgttgtgcct
     3841 caccacccga cagggtggtt gctgcttgtc ccaaggtaat ataacttaaa cccacggcca
     3901 ttaaggtctg taatttgcgg gcaaggctag gcacactttc aaaaaaggca aaggcatctt
     3961 ctaccgtcat atttaatact tgagaaatat ttttgccttt ataacgaatt tctaaggttt
     4021 cgcggttata acgttcgccg tggcacacat cacaaggcac atacacatca ggtaaaaagt
     4081 gcatggccac tttaatgaca ccatcacctt cacaggcttc acaacggcca cctttaacgt
     4141 taaacgaaaa tctgcccaca ctataacccc gtgagcgtgc ttcttgagtg gcggcaaata
     4201 gttcacgaat gggcgtaaat aaacccgtat aagtggctgg attagaacgc ggtgtacgac
     4261 caatcgggct ttgatcaata tcaaccactt tatctaaatg ttctaaaccc gtaatgcttt
     4321 tgtagggggc agggtttaag cttaatgcgc catttaaagc tttggcggta atgggaaata
     4381 atgtgccgtt aattaacgtc gattttcctg agccagaaac gcccgtcaca caggtcatta
     4441 aacctaaagg aatatcaatg ctgacatttt taaggttgtt gcctgtcgca cctgtcaaac
     4501 tgagaatttt ttcaggatta atggctgtgc gttggctagg aacagcaatt ttggctttgc
     4561 ctgataaata ttggccagtt aaggattcgg gaatttgttt aatgtcttca gctgtacctt
     4621 gggcgataat ttgaccacca tgtacacctg ccattgggcc aatatcaata atatggtcgg
     4681 cagcacgaat ggcatcttca tcatgttcaa ccaccagtac agtattacct aagtcacgta
     4741 aacgcagcag cgtgtttaat aaacgctcat tgtcgcgttg atgcaaacca atcgacggct
     4801 catctaacac atacattaca cccatcaagc ccgcaccaat ttgtgaggcg aggcgaatac
     4861 gttgtgcttc tccacccgat aaggtgtcag cagagcgttc taaagacaaa taatccaaac
     4921 caacactcac caaaaaatgc aaacggtctc gaatttcttt taaaaccttg tcggcaattt
     4981 cggctttttg ccccgttaag gccagatttt caaaataatc agccgctttt ttaatcggta
     5041 gttgggtaat gcttggcaag tttttgtcgg caacatacac atagcgtgct tctttacgca
     5101 aacgtgaacc atcacattga tcacaacgac tggtgattaa atattgactc agctcttcac
     5161 gcacagcagg cgattcggtt tctttataac gacgttctaa atgaggcaaa ataccttcaa
     5221 aaggttgttc gcgtaggcgt ttgttgccac gttcatcggt ataagcaaaa cttattttct
     5281 cttttttcga gccttgtaaa aagatcgttt gaatggcagc aggaagatct tgccatgctt
     5341 gatccatatc aacctgataa tgtttggcaa cactactaag caataaaaaa tagtagggat
     5401 gttgtttgtc ccaaccacga acgacccctt cgtttagact taattcggga tgtaaaatta
     5461 attgttcggc ggcaaagtgg gctttatgac ccaaaccatc acaacttgga caagctcccg
     5521 caggattatt aaaagaaaat aagcgaggtt ctaattcact aactgcataa ccacaatgcg
     5581 ggcaactatg tttagaggag aagattaagt cctcaccatc gttatccata taactaatca
     5641 tcgccacgcc atcagctaaa cgtaaggccg tttcaaaaga ctctgctaaa cgtaaagcta
     5701 aatcagcacg gactttaaaa cgatcaatca ccacttcaat ggtatgtttt ttggtttttt
     5761 ctaaagcagg ggcatcatct aaatcaacga caatgccatt aattcgtgct ctaacaaaac
     5821 cttgtgcgcg taaactctct aaaatatgta aatgttcacc tttgcgatct ctaaccacag
     5881 gcgcaagtaa catcaacgct gtgccttcgg caatggctaa aacattgtcc accatttggc
     5941 taatggtttg tgcacttaaa ggcaggttat gttcagagca atagggagtc cctacgcgtg
     6001 catataatag acgtaaataa tcataaattt cggtaatcgt accaacggta gaacgtgggt
     6061 tatgcgaagt ggatttttgc tcaatcgcaa tagcaggtga caagccgtcg atgctatcca
     6121 catcaggttt ttgcatcaac gccaaaaact gtcgagcata agccgataaa gactccacat
     6181 aacgacgttg accttcggca tataaggtat caaaggccaa cgatgatttg cccgaacctg
     6241 ataaacctgt aatgaccacc aatttgtcac gtggaatatc taaagaaatg ttttttaaat
     6301 tatgggtacg agcaccacga atacgaataa agttgttcat cttctatcaa cgcatcaagc
     6361 aaagagccga taataatggc ataacggagg tatttaagca ctatacaaag ataaaacttg
     6421 tatgagaccc aactatagag tggtattttt tcttagacga cggtcttata atacgcgacc
     6481 caaaattttt agagtgtcat taatgagcgg tatgactcaa ggcgaacgta aagcaaccag
     6541 tattttagcg agtatttttg cgctaagaat gttgggctta tttatgatta ttcctgtttt
     6601 tgccttgtat ggtcaaaaat tagagggtgc aacaccagct ttaattgggt tggcaatcgg
     6661 tatttatggt ttaacccaag cggtattaca aattccagtc ggtttatggg cagatagaat
     6721 caatcgcaag ttattaatta ttattggtct tattttattt gctattggtg gcgcagtagc
     6781 tgctatgtct catagtattt ggggagtaat tcttggtcga gctattcaag gtgcaggggc
     6841 tatttcggcg gtggttatgg cattattggc cgattta
//
LOCUS       DAOQMB010000084         6788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4699_length_6788_cov_14.305510, whole genome shotgun sequence.
ACCESSION   DAOQMB010000084 DAOQMB010000000
VERSION     DAOQMB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4699_length_6788_cov_14.305510"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLY05_04545"
     CDS             complement(<1..444)
                     /locus_tag="PLY05_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HNC02605.1"
                     /translation="MKSIRQYLLLGLFGTLLLSSCASFLISYISTTEEIGELHDAQLI
                     ENSRFIEGFLNQKPHKLNIEQINQALSQVKATHLEEGEDFNADTHAYERKVAIQVWDK
                     HGKLLLSTPSAPDHALSPLQKGFFQHKTGKHTWYIYTHQIPANQYW"
     gene            complement(441..1103)
                     /locus_tag="PLY05_04550"
     CDS             complement(441..1103)
                     /locus_tag="PLY05_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017394844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC02606.1"
                     /translation="MRILLVEDDLMLGQTLQRALEPQGYTVDWLRDGSQALQAILDQH
                     IDLIILDLGLPKIDGMSVLKQARLKGIQTPVLILTARDTTQDKVQGLDAGADDYLLKP
                     FDLAELYARIRALTRRANGRAESQICYGDLRLEPQNQRVFYQDKEISLHRREFMVLHE
                     LLENVGKVLTRQKLEQSLYGWGEDIESNALEVHIHHLRKKLYPELIKNLRGIGYIIEK
                     HK"
     assembly_gap    1119..1218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1365..1781)
                     /locus_tag="PLY05_04555"
     CDS             complement(1365..1781)
                     /locus_tag="PLY05_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC02607.1"
                     /translation="MYLLDTNVISEIRKQHKANTGVIQFFKTAQANNAELFLSVVTIG
                     ELRRGVDLIRHRGDIVQANLLEQWLDQISIQYQKHILSFDVNAAQIWGRLRVPNPEHS
                     LDKQIAATALMYDLILVTRNTADFAGTGVKLLNPFD"
     gene            complement(1768..1977)
                     /locus_tag="PLY05_04560"
     CDS             complement(1768..1977)
                     /locus_tag="PLY05_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02608.1"
                     /translation="MLPITAIQNFDSLPPIAQEQVIAFIDFLKNRYATKSPDCFIDSL
                     MAMPNVGKDSDFERVQNESTPDVFT"
     gene            complement(2080..2982)
                     /locus_tag="PLY05_04565"
     CDS             complement(2080..2982)
                     /locus_tag="PLY05_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004827088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC02609.1"
                     /translation="MSHDRSNQIHIQHHHNYWQIQFEAMASTCEVLVDTDDSQLVQRL
                     AHLISKETWRIEEKFSRYRQDNIVYKINHCEGGIVQVDAETAELIDFAAQCYALSGGM
                     FDVTSGILRRAWRFDGSSLLADPEVVESLLPHIGWEKVRWLNPVISLPYGMELDFGGI
                     GKEYAVDKVWLLATEHTQEPVLINFGGDLRVSGARRDGSPWKVGIENPEQDGETQGIL
                     EISQGALATSGDSKRFLLKDGVRYSHILNPMTGWPITDAPHSVTVAAPTCIEAGMMAT
                     FASLQGEFAEQFLEAMDTPYWCIR"
     assembly_gap    2985..3084
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3085..4034)
                     /locus_tag="PLY05_04570"
     CDS             complement(<3085..4034)
                     /locus_tag="PLY05_04570"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HNC02610.1"
                     /translation="MQLTNKKMPAFRAALAAASCGLLGIATTTAHAEDTSADSKKWQV
                     DTAVLYYAEDNGRVTAAEPIVNFKKDYGDERVLNLKVTLDSLTGASPNGATSSSQPQT
                     FTGPSGNKTYTTAPNSQPLDGEFKDTRAAIHANWEQPLGEDYKASVGGGISSEYDFKS
                     LSLNGAISKDLNNKNTTISAGLNLELDTLNSVGGKPKPFSSMAANIKEGDDNKTVIDA
                     LIGVTQVINRKTVMQWNYSLSTSSGYHTDPYKVLSLVDGTTGFASDYLFENRPDSRTR
                     HSLFWQTKYHLTEDVVDVSYRYMTDDWGINSHTLDLRYRY"
     gene            complement(4025..>4252)
                     /locus_tag="PLY05_04575"
     CDS             complement(4025..>4252)
                     /locus_tag="PLY05_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HNC02611.1"
                     /translation="MKYKLSLLILSGVLLSSLTACSSLGVKPWERDLLAKKSMQLNSA
                     PLDSAIDDHIYFSKEASSGGRAFGGGGCGCN"
     assembly_gap    4253..4352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4353..4795)
                     /locus_tag="PLY05_04580"
     CDS             complement(<4353..4795)
                     /locus_tag="PLY05_04580"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNC02612.1"
                     /translation="MNKIVKWLGASLLALSSTLTHADTLNLDQYKGKVVYVDFWASWC
                     GPCRESFPWMKKMQQQYGKDGLVIIAINVDQDKKLADKFLSEFKPEFNVLFDKDGTLA
                     EDFKVSSMPSSYVLDREGKPRFKHKGFHLDKQSQYETELNSLLKN"
     gene            5068..>6788
                     /gene="dsbD"
                     /locus_tag="PLY05_04585"
     CDS             5068..>6788
                     /gene="dsbD"
                     /locus_tag="PLY05_04585"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNC02613.1"
                     /translation="MRLWPILLLATSINSVQAAVPSFDDIPAEEDYLEVGQAFQPVVK
                     TTDGHVQMSFTIADGYYLYKKRISIQSTDNNHPVVMGDGQFSQKAEIKNDPNFGRVEI
                     FHKTLAVEIPVYAQNPQDVGQITTVKAIYQGCSQQGLCYPPQTIKANVKLLQLAPTPT
                     VDTDNLTTVNNSASVGSKKTDTNDANSLSQFLAGSSLGLILATFLVLGIGLSFTPCVL
                     PMVPILSGIIAGQGQGLSAKRGFLLSLSYVVGMATTYALAGMAVGYFGASANLSAWMQ
                     SPVVLSVFAGIFVLLALAMFGFYELRLPAFIQDRLSNLNQKQQGGTYFGVLLMGILSA
                     LVVSPCVSAPLAGALLYISSTGDMWLGGAALLALGLGMGTPLLLIGVGGGSLIPKAGS
                     WMVVVKSVFGVLLLMVAIWLLSRFLAGAVTLVLWGLLLIVCGILMGALDNTQAGWQRF
                     WKGLGVALLVWGVLLLVGAANGQSNPLRPLQFSSTSNKELSNKTELTFRKVNNAQELE
                     VALAEAKQQGKVALLDFYADWCVACVEMAHDTFSSPIVANALKPVVLLQADITSNDDN
                     AQAMLKR"
BASE COUNT         1847 a         1245 c         1338 g         2058 t
ORIGIN      
        1 ccaatattgg ttagcaggaa tttgatgggt ataaatgtac caagtatgtt tacctgtttt
       61 gtgttgaaaa aaaccctttt gtaatgggga gagtgcgtga tctggtgcgc tgggtgtact
      121 taataataat ttgccatgtt tgtcccatac ttgaatggca actttacgct cataagcatg
      181 ggtatctgca ttaaagtctt caccttcctc taaatgggtt gctttgactt gggagagtgc
      241 ttgattgatt tgttcaatat tcagtttatg cggtttttga ttaagaaagc cttcaataaa
      301 tcgagagttt tcaatcaatt gtgcatcgtg caactcacca atttcttcag tggtagatat
      361 atagctaatt aaaaatgagg cacaagatga caatagcaat gtgccaaata agccaagtaa
      421 caaatactgg cgaatcgact tcatttatgt ttctctataa tgtagccaat accacgtaaa
      481 tttttaatca gttcgggata gagttttttg cgtaaatgat gaatgtgtac ttctaaggca
      541 ttgctttcaa tgtcttcgcc ccatccatat aaactttgtt cgagtttttg acgagtaagg
      601 actttgccaa cattttcgag tagttcgtgt agcaccataa attcacggcg gtggaggcta
      661 atttctttat cttgataaaa aacacgttga ttttgtggtt ctaaacgcaa atcgccataa
      721 caaatttggc tttcggctcg gccatttgca cgacgagtta aagcacgaat ccgtgcatag
      781 agttctgcta aatcaaaggg ttttaataaa taatcgtctg cgcctgcatc caaaccttgt
      841 actttgtctt gagtggtatc gcgtgcagtt aaaattaata cgggtgtttg aatgccttta
      901 agtcgggctt gtttaagtac actcatgccg tcaattttgg gcaagcctaa atctaaaata
      961 atcaggtcta tgtgctggtc taaaatggct tgtagtgctt ggctaccatc acgtagccaa
     1021 tccacggtgt aaccttgtgg ctctaaggcg cgttgtaagg tttgtccaag catcaaatca
     1081 tcttcaacta ataaaatacg catacgccat aaccctatnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnat gttttgtaat gctttagctt aaatcggctt tggcagcatc
     1261 atacaataaa tgacaaaact taaggattgc ttaaggcaat ataatttttg taggcttggt
     1321 gggggagatt gtgggtttag gctaaaccca caataattcg gcttttaatc aaatggattg
     1381 agcaatttga caccagtccc tgcaaaatcg gctgtatttc gtgtcactaa aattaagtca
     1441 tacattaatg ccgttgcggc tatttgtttg tctaaactat gctcaggatt tggaacacgt
     1501 aaacgtcccc aaatttgagc agcgttgaca tcaaaagaca atatgtgttt ttggtattgt
     1561 atcgatattt ggtctaacca ttgttctaaa aggtttgctt gaacaatatc accacgatga
     1621 cgaattaaat caacgcctct ccttaattcg ccaatagtga cgacacttaa aaataattca
     1681 gcattgtttg cttgagccgt tttaaaaaat tggataacac ctgtattcgc tttatgttgt
     1741 ttgcggattt cactaatcac attggtatca agtaaataca tcgggtgtag actcattttg
     1801 tacacgttca aaatcactat ctttacccac atttggcata gccattaatg agtcaataaa
     1861 acaatcaggt gattttgtcg cataacgatt ttttaaaaag tctataaaag caatgacttg
     1921 ctcttgagca attggcggca aactatcaaa attttgtatg gcagtgatag gcaacatggt
     1981 ctttactcct aagagtggct ttgttttatc atagcattta ggataattag cagcaaagtt
     2041 gtagcgtggg ttgaggcaca aaacccacga taattctaat taacgaatac accaataagg
     2101 cgtatccatt gcctctaaaa actgctcagc aaattcacct tgtaaactcg caaaagttgc
     2161 catcatgcct gcttcaatac acgtaggggc agcgactgtc acggagtggg gcgcatcagt
     2221 aattggccaa cctgtcattg ggtttaaaat atggctataa cgcacaccat cttttaacaa
     2281 aaaacgtttg ctatcaccac tggtcgccaa cgcgccttgg ctaatttcta aaataccttg
     2341 tgtttcgcca tcttgttctg ggttttcaat gcccactttc cacggtgaac catcacgacg
     2401 cgcacctgac acacgcaaat caccgccaaa attaattaat acaggctctt gggtatgctc
     2461 agtggctaat aaccaaactt tatcaaccgc atattctttg ccaatgccac caaaatctaa
     2521 ctccatgcca tagggaagag aaataacggg atttaaccaa cgcacctttt cccaaccaat
     2581 atggggtaat aaagactcaa ccacttcagg atcagccaac aaactactac catcaaaacg
     2641 ccatgctcga cgcaaaatgc ccgatgtcac atcaaacatg ccaccactta aggcataaca
     2701 ttgagctgca aaatcaatta actcagccgt ttcggcatcc acttgaacga tgccaccttc
     2761 acaatgatta attttataga caatattatc ttggcgataa cgactgaatt tttcttcaat
     2821 gcgccatgtt tctttgctaa ttaaatgggc gagtcgttgc acgagttgac tatcatcagt
     2881 atcaactaac acctcacagg tgctagccat tgcctcaaat tgaatttgcc aataattatg
     2941 atgatgttga atgtgaattt ggttactgcg gtcgtgggac atagnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnntgataa cgataacgta aatcgagcgt atgggagtta
     3121 atgccccaat catcggtcat ataacgataa gacacatcaa ccacatcttc ggttaaatga
     3181 tatttggtct gccaaaataa actatgacgt gtgcggctat caggacgatt ttcaaataaa
     3241 taatctgacg caaaccctgt ggtgccatca accaagctta agactttata agggtcagta
     3301 tgataaccac tggatgtgct taacgagtaa ttccattgca taaccgtttt acggttaatc
     3361 acttgggtaa caccaattaa ggcatcaatc accgttttat tatcatcacc ttctttgata
     3421 ttagccgcca tagaactaaa gggcttaggt ttgccaccca cagagttaag cgtatctaat
     3481 tctaggttta aacctgcact gatcgtggtg tttttgttgt ttaagtcttt agaaatcgcc
     3541 ccatttaacg ataacgattt aaagtcatat tcagaagaaa taccaccacc aacactagct
     3601 ttgtagtctt cgcctaacgg ttgttcccag ttagcatgaa tggccgcacg ggtatcttta
     3661 aactcaccat ctaaaggttg actatttggt gcagtggtat aggtcttatt accagaaggg
     3721 ccagtaaagg tttgcggctg gctactggag gttgcaccat taggcgatgc acctgttaag
     3781 gagtctaacg tcactttgag attaagcaca cgctcatcac cgtagtcttt tttaaagtta
     3841 acaatcggtt cagcagccgt cacacgacca ttatcttcgg catagtacaa aacagcagta
     3901 tctacttgcc attttttgct atcggcagaa gtatcttcgg cgtgagccgt tgtagtagca
     3961 attcctaata aaccgcagct tgctgcggcc aatgcggcac gaaaggctgg catctttttg
     4021 ttagttagtt gcatccgcag ccacctccac caaatgcgcg accaccactg gacgcttctt
     4081 tactaaaata aatatggtca tcaatcgcgc tgtctaatgg cgcactattg agttgcatgg
     4141 actttttcgc taacaaatcg cgctcccaag gtttgactcc taaagagcta cacgctgtta
     4201 agctgctgag tagcacacca ctaaggatga gtaggctgag cttgtatttc atnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntagttttt taataaactg ttaagttcgg
     4381 tttcgtattg gctttgttta tcaagatgaa aacctttgtg tttaaaacga ggttttcctt
     4441 ctcggtctaa tacataagag ctaggcattg aactgacttt gaagtcttcg gctaatgtgc
     4501 cgtctttatc aaaaagaaca ttaaattcgg gtttaaattc acttaaaaat ttgtcagcca
     4561 gttttttatc ttggtcaacg ttaatagcaa taatcactaa accatctttg ccatattgtt
     4621 gttgcatctt tttcatccac ggaaaagatt cacggcaagg gccacaccaa gatgcccaaa
     4681 agtcaacata aaccactttg cctttatatt ggtctaaatt aagcgtatcg gcatgagtca
     4741 aggtgctact taaggctaat aaacttgcac ctaaccactt gacaatttta ttcattatct
     4801 aatacctatt cattgaatgc tgatgagcat tacttgtctg agcatgaggc tattctaatc
     4861 ggttaaatct taaggattac ttaaggaaaa ttaattttat tcataattat aaaaatgatg
     4921 atatttgtat aaatgcatga atataaatta aattttagtt ttttcctgtt ttaaacgtta
     4981 tttctatttc ttacttttct taagactctc ttaatgaatg cttgttacat tgtttgctag
     5041 aagaaattaa tttataaggg tgaaaatatg cgcctttggc ctattttgct gttagcaacg
     5101 tctataaata gtgtacaagc tgctgtgcca agttttgatg atattcctgc tgaagaggat
     5161 tatttagagg ttggccaagc gtttcaacct gttgtcaaaa cgacagatgg ccatgtgcaa
     5221 atgagtttta ccattgctga tggttattat ttatataaga agcgtatttc gatacaaagt
     5281 acagataata atcatcctgt agtcatgggt gacggccaat ttagccaaaa agccgaaatt
     5341 aaaaacgacc caaattttgg tcgggtagaa atttttcata agactttagc tgttgaaatt
     5401 cctgtgtatg cccaaaatcc tcaagatgtg ggtcaaataa caacggtcaa agccatttat
     5461 caaggttgtt cacaacaagg tttatgttat ccaccacaga caattaaagc caatgttaag
     5521 cttttgcagt tggcacccac acccactgtt gacacagata atttgaccac agtgaataat
     5581 tcagcaagtg tgggtagtaa aaaaacggat accaatgatg ccaatagttt atcgcagttt
     5641 ttggcaggta gtagtttagg gctgattttg gccacatttt tggtgttggg gattggctta
     5701 tcatttactc cctgtgtgtt acccatggta ccaattttgt caggcattat tgctggccaa
     5761 gggcagggct tatcggctaa acgtggtttt ttattatcgt tgtcttatgt ggttggtatg
     5821 gcaaccactt atgctttagc agggatggcc gttggttatt ttggcgcaag tgctaattta
     5881 tcggcatgga tgcaaagccc tgtggtgttg tcggtgtttg cagggatttt tgtgttgttg
     5941 gctctagcaa tgtttggctt ttatgaatta cgtttacctg catttattca agatcgttta
     6001 agcaatttaa accaaaaaca acaaggtggc acgtattttg gtgtgttgtt aatggggatt
     6061 ttgtcggcat tagtggtatc accttgtgtg tctgcaccgt tggcaggtgc gttgttatac
     6121 atcagtagta cgggggatat gtggttaggt ggtgcagcat tattggcttt aggtttaggc
     6181 atgggcactc ccttattatt aattggtgtc ggtggcggta gcttgattcc aaaagcaggc
     6241 tcatggatgg tagtggttaa gtcggtgttt ggtgtgttgt tgttgatggt tgccatttgg
     6301 ttgttatcac gctttttagc gggagcagtc actttagtgt tatggggttt gttgttgatt
     6361 gtctgtggca ttttgatggg agctttagac aatacccaag caggatggca acgtttttgg
     6421 aaaggtttag gtgttgcatt attggtatgg ggcgtgctgt tgttggtggg cgctgccaat
     6481 ggtcagtcta atcctttaag acctttacag ttttctagta caagcaataa agagttgagt
     6541 aacaaaaccg aattaacctt tcggaaagtc aataatgccc aagaattaga agtcgcttta
     6601 gctgaggcaa aacaacaagg taaagtggct ttattagatt tttatgccga ttggtgtgtg
     6661 gcttgtgtgg aaatggcaca cgatacgttt agctcgccaa ttgttgccaa tgctttaaaa
     6721 cctgtggtgt tattacaagc agatattacc agcaatgatg acaatgcaca ggcgatgtta
     6781 aaacgttt
//
LOCUS       DAOQMB010000085         6755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4732_length_6755_cov_14.482388, whole genome shotgun sequence.
ACCESSION   DAOQMB010000085 DAOQMB010000000
VERSION     DAOQMB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4732_length_6755_cov_14.482388"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1134
                     /locus_tag="PLY05_04590"
     CDS             <1..>1134
                     /locus_tag="PLY05_04590"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNC02614.1"
                     /translation="IDLFDKVLLQAVSPEPEPVDELVTDNKGRAVIVVVDAGHGGEDS
                     GAIGATGNYEKHVTLAIAKKLVALLRKEKGFKAYLTRDDDYFIPLQDRRKIARNRYKA
                     DIFISIHADAALSPLAKGASVFALSRKGANSATSRFAQALADRENKSDLLGGVDNSFG
                     KDLQLNNILADMVVEGTVTQSLHMGSYILKEMDDLTKLHSKRVEQAGFAVLKEAGMIS
                     LLVETGFMTNVEEEAKLIQSSYQQQMAEAVFVGLRRFVQKYPIPQTYFAVGKEQKNRK
                     IRDRALQEIAPPAPKPINNQPEKLLDKPIDNKAIINSFNQEIEPTKNKPVDDKVVKQP
                     EKTIDPKPMVKATLPSSLDEFMAGSMTTTTPVAKEVKPEKPVVK"
     assembly_gap    1135..1234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1315..3153
                     /gene="mutL"
                     /locus_tag="PLY05_04595"
     CDS             1315..3153
                     /gene="mutL"
                     /locus_tag="PLY05_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNC02615.1"
                     /translation="MKRIHRLDAQLANQIAAGEVVERPASVIKELLENAIDAGATQID
                     ITIEEGGLGLLQVRDNGSGIHPDDLPLALARHATSKIYALDDLDAVSSLGFRGEALAS
                     IASVSRLSLTTSHQEKGIGWCVQSEGRDMTAVVTPANHPRGTTVSVRDIFFNTPARRK
                     FLKSPTTEFSHIEEVVKRLSLANFDIGFVLQHNGNRRWQFKPALNDLEKLRRVSTLCG
                     QSFLEAAIPIDVEGTGLQLYGWLGSPSMARGQADMQYFYVNGRVVRDKVVSHAIRTAY
                     SDVLAPNRHPAFVLFLELDPSAVDVNVHPTKHEVRFREQRWVHEFLARSIHKALSALK
                     TEQTFAPPINQENQSFAAPSLSQQTPLALPTSNASLYSGGGDSGFYRPATPSVQGFSQ
                     EALADYLAPLREVMQPVSLPVTATPDIPPLGYALAQLHGIYVLAQNSQGLIIVDMHAA
                     HERLVYENLKKAWQTGRLASQPLLLPQTIAVSKNEAELAEQAQTWFSQLGLEIDRIGQ
                     EALAVRAIPALLQKSDLTGLVRDVLADFKEHGQSQRIEEAINELFASMACHGAVRANR
                     ALSLPEMNALLREMEATEFSSQCNHGRPTWKQFSLADLDKLFWRGR"
     gene            3265..3954
                     /gene="cmk"
                     /locus_tag="PLY05_04600"
     CDS             3265..3954
                     /gene="cmk"
                     /locus_tag="PLY05_04600"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNC02616.1"
                     /translation="MTVPVITIDGPSGSGKGTIAHRVALKLGFHVLDSGALYRLLALA
                     AQRHGFSLDNEDALEVLAAHLDVQFQDNHGEAMLILEGQDVTLEIRTEEVGAAASKVA
                     ALPRVRSALLQRQHAFRESPGLVADGRDMGTVVFTDACLKVFLTASAEARAERRYKQL
                     KDKGFSADLEIILADIRARDLRDSQRAVAPLKPATDAIVLDSTALGIEAVLAAVLQEA
                     KKQSSLQQFFA"
     gene            4122..5798
                     /gene="rpsA"
                     /locus_tag="PLY05_04605"
     CDS             4122..5798
                     /gene="rpsA"
                     /locus_tag="PLY05_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HNC02617.1"
                     /translation="MSESFADLFAESELQFTQGAVISGVVVAIDSDWVTVDTGLKSEG
                     VVPREEFLNDLREIEVKVGDTVEVVVDAFDNGMGQTMLSREKAKRAETWTRLEKIFTD
                     GEIVRGLISGKVKGGFTVDIGPIRAFLPGSLVDTRPIRDTAHLEGKELEFKVIKLDQK
                     RNNVVVSRRAVMEIASDDVRRALLDQLEEGKVVKGTIKNLTDYGAFVDLGGIDGLLHI
                     TDMAWKRVKHPSEVVEVGQEMEVKVLKFDRERNRVSLGLKQLGQDPWLALMSKYPKGT
                     ITRAKVTNLTDYGCFAEIAEGIEGLVHVSEMDHTNKNIHPSKVVQIGDEVDVMVLEID
                     EERRRISLGIKQCKANPWDEFAKTHEKGQKVSGNIKSITDFGIFIGLPGGIDGLVHLS
                     DISWNEAGEEAIRKFRKGDLVEAVILAVDAEGNRISLGIKQLAKDPFSDFAASHEKGA
                     VVKGTVKAVDAKGATVELAEGVEANLKASEIVRDRVEDASKHLTVGQEIEAKIIGVDR
                     KARVINLSIKAKDEAEEREAMSNMRNQADAAATAAGPRTIGDLIKEQMKK"
     gene            6120..6428
                     /locus_tag="PLY05_04610"
     CDS             6120..6428
                     /locus_tag="PLY05_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004679473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HNC02618.1"
                     /translation="MSLTKSELISKVAARQSLSLLTEKDIELAIKALLEQMVNALAEG
                     QRVEIRGFGSFSLHHRPPRVGRNPKTGESVSVGSKYVPHFKPGKELKERVNQSIEAGK
                     "
     gene            6433..6735
                     /locus_tag="PLY05_04615"
     CDS             6433..6735
                     /locus_tag="PLY05_04615"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HNC02619.1"
                     /translation="MVWSRIKKILVVFCWLSLAFYGLWVVVSNQAQISVNLLFVEIGA
                     LNTGLALVISFVVGSLFGVLASLFIWGVIPLRWQLRQTHKELTALRKQTVRPPVEP"
BASE COUNT         2001 a         1214 c         1499 g         1941 t
ORIGIN      
        1 attgatttat ttgataaagt tttactccaa gccgtttcac cagagccaga acctgtagat
       61 gagttggtca ctgataataa aggccgtgcg gtgattgttg tggttgatgc aggacatggt
      121 ggtgaagatt ctggcgcaat tggcgcaaca ggtaattatg aaaaacatgt cactttagcg
      181 attgccaaaa agcttgtggc tttattgcgt aaagaaaaag gctttaaggc atatttaaca
      241 cgagatgatg attattttat tcctttgcaa gaccgtcgta aaattgctcg caatcgttat
      301 aaagccgata tttttattag tattcatgct gatgcagctt tgtctcctct agcaaaaggt
      361 gcatcagtgt ttgccttgtc tcgaaaggga gctaattctg caacctcacg ttttgctcaa
      421 gcattagccg atagagaaaa caaatcagac ttactgggtg gtgttgataa tagttttggt
      481 aaagatttgc agttaaataa tattcttgcc gatatggttg ttgaaggaac agtcacccaa
      541 agtttgcaca tgggttcgta tattttaaaa gaaatggatg atttaaccaa attacattcc
      601 aaacgagtcg aacaagcagg ttttgccgtt ttaaaagaag caggtatgat ttccttattg
      661 gtcgaaacag ggtttatgac taatgtggaa gaagaggcta aattaattca atctagctat
      721 caacagcaaa tggcagaagc ggtatttgtg ggtttacggc gatttgtaca aaaatatccc
      781 attccacaaa cttattttgc ggtgggtaaa gaacaaaaaa atcgtaaaat cagagatagg
      841 gctttacaag aaattgcacc gccagcacct aaaccaatta ataatcagcc tgaaaaatta
      901 ttggataaac caattgataa taaagcaatt attaacagtt ttaatcaaga aatagaacca
      961 accaaaaata agccagttga tgacaaagtg gtcaaacaac cagaaaaaac tatcgaccct
     1021 aaaccaatgg ttaaagccac cttgccgagt tctttagatg aatttatggc aggaagtatg
     1081 acaacaacga cacccgttgc caaagaagta aagccagaaa agcctgttgt taaannnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcagcg caagccatta aagatttaaa
     1261 taaattaaaa gacgataatg ttgttttaga taaaacctta aagattccta caccatgaag
     1321 cgtattcatc gtttagatgc ccaactagct aaccaaattg ctgcgggtga agtggttgaa
     1381 cggccagcct ctgtgataaa agaattatta gaaaatgcca ttgatgcagg ggcaacacaa
     1441 attgatatta ccattgaaga aggcggttta ggactgctgc aagtgcgtga taatgggagt
     1501 ggtattcatc ctgatgattt gcctttagcc ttagcgcgtc atgcgaccag taaaatctat
     1561 gccttggatg atttggatgc agtgagcagt ttaggttttc gcggtgaggc tttagcgtct
     1621 atcgcctcag tatcacgttt aagtttaacc acaagtcatc aagaaaaggg tatcggctgg
     1681 tgtgtacaaa gcgaaggtcg agatatgacc gctgtggtca cacctgctaa tcatccgcgt
     1741 ggcacaacgg tcagtgtgcg agatattttt tttaatacac ctgcgcgccg taaattttta
     1801 aaatcgccaa ctaccgagtt ttcacatatt gaagaagtgg ttaaacgttt aagtttagcc
     1861 aattttgata ttggttttgt tttgcaacac aatggcaatc gccgttggca atttaaacct
     1921 gcacttaacg acttagaaaa attacgtcgg gtgagcactt tatgtggcca aagcttttta
     1981 gaggccgcta ttccaattga tgtcgaaggg actggtttgc aattgtatgg ttggcttggt
     2041 agtcccagca tggcacgcgg ccaagctgat atgcagtatt tttatgtgaa tggtcgagtg
     2101 gttagagata aagtggttag tcatgcaatt agaaccgctt atagtgatgt tttagcccct
     2161 aatcgtcatc ctgcttttgt gttgttttta gagttagacc cttctgcggt ggatgtgaat
     2221 gttcatccga ccaaacatga agtccgcttt cgtgaacagc gttgggtaca tgagttttta
     2281 gcacgttcta ttcataaagc tttatcggca ctcaaaaccg aacagacttt tgcaccgccg
     2341 attaatcaag aaaatcaaag ttttgctgca ccaagcttaa gccaacaaac acccttggcg
     2401 ttgcctacta gcaatgcttc tctttatagt ggtggcggtg attcgggttt ttatcgacct
     2461 gcaacaccca gtgttcaagg ctttagtcaa gaagcattgg cggattattt agcaccatta
     2521 cgtgaggtga tgcagccagt tagcttacct gtgacagcaa caccagatat tccgcctttg
     2581 gggtatgcac ttgcacaatt acacggaatt tatgtcttgg cacaaaatag ccaaggcttg
     2641 attattgtgg atatgcacgc tgcccatgag cgtttggtgt atgaaaactt aaaaaaagca
     2701 tggcaaacag gtcgattagc ctcacaacct ttattgttgc cacaaacgat tgccgttagt
     2761 aagaatgaag cagagttagc cgaacaagct caaacatggt ttagtcaatt aggtttagaa
     2821 atagatcgaa ttggccaaga agcattggcc gtcagagcca ttcctgcttt acttcaaaaa
     2881 tctgatttaa caggattagt cagggatgtt ttggcggatt ttaaagagca tggtcaatca
     2941 caacgcatcg aagaggccat taatgaattg tttgcgagca tggcttgtca tggtgcagtc
     3001 cgcgccaatc gtgctttatc gttacccgaa atgaatgctt tattacgaga aatggaagca
     3061 acagaattta gtagtcaatg taatcatggt cgacccacat ggaagcagtt tagtttagcc
     3121 gatttagata aattgttttg gcgagggcgt taaacgcttg caattttgat aaaaaatcta
     3181 taaaaagccc acgctttatt tttgattagg agccacacca tgagtgctga tttagagcct
     3241 aaacaagcca attatagtca gccaatgact gttcctgtta ttaccattga tgggccaagt
     3301 ggttcgggta aaggcacaat tgcgcaccgt gttgccttaa aattgggttt tcatgtttta
     3361 gattcaggtg ctttatatcg tttattagcg ttagccgctc agcgtcatgg tttttcgtta
     3421 gataatgaag atgctttaga agttttggcg gcacatttag atgtgcagtt tcaagataat
     3481 catggtgagg cgatgctcat tttagaaggg caagatgtca cactcgagat tcgtaccgaa
     3541 gaagtgggtg ctgctgcttc taaagttgca gctttgccaa gagtgcgctc agccttatta
     3601 caacgtcaac acgcttttcg cgaatcaccg ggtttagttg ctgatggtcg tgatatggga
     3661 acggtcgtat ttaccgatgc ttgtttaaaa gtatttttaa cagccagtgc agaagcccgt
     3721 gcagaacgtc gatataaaca actcaaagat aaaggttttt ctgccgattt agagattatc
     3781 ttggctgata ttcgtgcgcg tgatttgcgc gacagccaac gtgcggttgc accattaaag
     3841 cctgctacag atgccatcgt tttagacagt acggctttag gtatagaagc tgttttggca
     3901 gccgtattac aagaagccaa aaaacaaagc agtttgcaac aattttttgc ttaaatcctc
     3961 aacaagctgt caaagctcgt gtataatttg gcactttttt aaaatcatca agggcattaa
     4021 gcacatacca gcccagtgtt tattggctct gaataacaac agcccgacat gagctggtca
     4081 tggcggtcaa caaagatgct taggcatcaa aaggtagaat tatgagcgaa tcatttgccg
     4141 atctctttgc agaaagcgaa ttacaattta cccaaggcgc tgttatttct ggtgttgtcg
     4201 ttgcaatcga cagtgactgg gtaactgtag acacaggttt aaaatctgaa ggtgtagtgc
     4261 cacgcgaaga gttcttgaat gacttacgcg aaattgaagt aaaagtaggc gacaccgtag
     4321 aagtggttgt tgacgctttt gataacggca tgggtcaaac catgttgtct cgcgaaaaag
     4381 ctaaacgtgc agaaacatgg acacgtttag agaaaatctt caccgatggc gaaatcgtac
     4441 gtggtcttat ttctggcaaa gttaaaggtg gctttaccgt tgatatcggc ccaatccgcg
     4501 cattcttacc aggctcttta gttgatacac gtccaatccg tgatacagct cacctcgaag
     4561 gcaaggaatt agaattcaaa gtcatcaagt tagaccaaaa acgcaacaac gttgttgttt
     4621 ctcgtcgtgc ggtaatggaa attgcttctg atgatgtaag acgtgcattg ttagaccaat
     4681 tagaagaagg caaagtcgtt aaaggtacta tcaaaaacct cacagactac ggtgcattcg
     4741 ttgacttggg cggcattgat ggcttgttac atatcactga tatggcttgg aaacgtgtta
     4801 aacacccaag tgaagtggtt gaagttggtc aagaaatgga agttaaagtt cttaagtttg
     4861 accgcgaacg taaccgtgta tccttaggct taaaacaatt aggccaagat ccatggttag
     4921 cattaatgag caagtatcca aaaggtacaa ttactcgtgc taaagtgact aacttaacag
     4981 actacggttg ttttgctgag attgctgaag gcatcgaagg tttggtacac gtatccgaaa
     5041 tggatcacac caacaaaaac attcacccat ctaaagttgt tcaaattggc gacgaagtgg
     5101 atgttatggt attagaaatt gatgaagaac gtcgtcgtat ttctttaggt atcaaacaat
     5161 gtaaagctaa tccttgggat gagtttgcta aaactcacga aaaaggtcaa aaagtttctg
     5221 gtaacatcaa gtcaatcact gacttcggta tcttcattgg tttaccaggt ggtatcgacg
     5281 gtttagtaca cttgtctgat atttcttgga acgaagctgg cgaagaagca atccgtaagt
     5341 tccgtaaagg tgacttagtt gaagctgtta tcttagctgt tgatgctgaa ggcaaccgca
     5401 tctccttagg tatcaaacaa ttagctaaag acccattcag tgactttgct gcttctcacg
     5461 aaaaaggtgc agtggttaaa ggcactgtaa aagctgttga tgctaaaggc gcaactgttg
     5521 aattagccga aggcgtagaa gctaacttaa aagcctctga aatcgttcgt gatcgcgttg
     5581 aagatgcttc taagcactta acagttggtc aagaaattga agctaagatc attggtgttg
     5641 accgtaaagc acgcgttatc aacttatcga tcaaagctaa agacgaagcc gaagagcgtg
     5701 aagctatgtc taacatgcgc aaccaagctg atgcagccgc aacagctgct ggtccacgta
     5761 caatcggtga cttgattaaa gaacaaatga agaagtaatt ctttaatatg ttagtgttaa
     5821 actagcataa atgttctaaa aacgggaatg gtgcaaacta ttcccgtttt tttattgcga
     5881 atcaagacta agtaacttgt cagtcaaagt cataattgct agtatcgtca atgtgttatc
     5941 gtttagctaa tcaactaata atggcaaatg tgtgtgtaac gataattaat ccgctcaaga
     6001 aatggcctaa agattgctag tgttttgaga tgctattaat aaagtagcca ttaataatca
     6061 taatttaacg gatttaccgt tttagtttct attttatttt tagaaagagg aacagaatca
     6121 tgtcattaac aaaatcagag ttgataagca aagttgctgc acgccagtct ttgtcattat
     6181 tgaccgaaaa agacattgaa ttagcaatca aagccttatt ggaacaaatg gttaatgcct
     6241 tggctgaagg tcagcgtgtt gaaattcggg gctttggtag ttttagtttg catcatcgtc
     6301 cgccacgtgt tggccgtaac ccaaaaacgg gtgaaagcgt ttctgtgggt tcaaaatatg
     6361 tgcctcattt taaaccaggc aaagagctga aagagcgcgt aaatcaatcg attgaagcgg
     6421 gcaaataagc aaatggtatg gtcgcgcatc aaaaaaatac tggttgtatt ttgttggtta
     6481 tctttggcat tttatggcct atgggttgtt gtcagtaatc aagctcaaat atcagttaat
     6541 ttattattcg ttgaaattgg tgcgcttaat acaggcttgg cattggttat cagttttgtg
     6601 gtggggagtt tatttggtgt tttggcctca ttatttattt ggggtgtgat accattacgt
     6661 tggcaattac gtcaaacaca taaagagttg acagccttac gtaaacaaac tgttcgtcct
     6721 cctgttgagc cttaagattt tatgtcctct gttac
//
LOCUS       DAOQMB010000086         6746 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4738_length_6746_cov_10.292632, whole genome shotgun sequence.
ACCESSION   DAOQMB010000086 DAOQMB010000000
VERSION     DAOQMB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4738_length_6746_cov_10.292632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1079)
                     /locus_tag="PLY05_04620"
     CDS             complement(114..1079)
                     /locus_tag="PLY05_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002061839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNC02620.1"
                     /translation="MAHLVETMAYVGATPWHGLGNHLEEQQPLEIWAKQAGMDWSILE
                     APVRFMTGDEQTSSIRTFADNKVLYRSDTHAPLSVVSQRYQVVQPREILEFYRDLTEI
                     SGFELETAGVLKGGRKIWALARTGQSMSLRGNDVTNGYLLLATACDGTLATTAQFTSI
                     RVVCNNTLAVALNGQGQAVKVPHNTTFDAQAVKKQLGVSVAAWDDFMYRLKTLSERKV
                     KSTEVDKFYAKVFGDAAMKGQASMQSFERTMAKAKTLFEGQGLGSDLSSAKGTAFGLL
                     NSVTEFVDHQRKARSIDHRLDSAWFGQGAIIKQRALDESLRLVSA"
     gene            complement(1236..1568)
                     /locus_tag="PLY05_04625"
     CDS             complement(1236..1568)
                     /locus_tag="PLY05_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02621.1"
                     /translation="MALQCPHCHSFQIDALNHGRKTGSAVGTVAGAASGASGSFTGAR
                     IGASVGAVAGPLGSVVGGLAGALFGGFIGGVAGGTAGSKLGEFVDDNILDNYQCLRCG
                     YTFSKRNE"
     gene            1953..3422
                     /locus_tag="PLY05_04630"
     CDS             1953..3422
                     /locus_tag="PLY05_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HNC02622.1"
                     /translation="MLLKGILMINNKLRTIPVPNKSDPVAFFQNAVKEVVQNTQAPED
                     MVQLCALAAISTVIQRLIKVKLPGVGVVPTSLMLMIVAKSGERKSAVESKFFNPIREF
                     EQSKRIVYEKDYQQWKAEHEVWEIERKAVQKKLDKAITTNAPSEEIDVIRAELTQKIA
                     QELKKPTPIKILYDDSTSQALYSGLKNGLLFAGLISSEGGSVLKSGVFSDMAKLNSLW
                     SGESITIDRVKAESIHISDARLTTLIMVQPEILADYMDKKGEQARGSGLIARFLFSSG
                     QSTQGTRLMEDYNQEPRWEYLERFNYLIKEFLESSYQSSLKEAFNPQILELSPAAEKL
                     WITYYNNIERHINIGGVYDSFGDHASKLAENAGRIAALLNYFHWNSNIIGSEVMEYAI
                     NCCDNLSKNFLVFFNVQPQEIQDAEKLLEWLNDTIKYQFHKSRSIKKNDILKYGPSFL
                     RKSERLNKALEVLLHNGSIYFFQDMRRTTYVGLNLYQIL"
     gene            4128..4817
                     /locus_tag="PLY05_04635"
     CDS             4128..4817
                     /locus_tag="PLY05_04635"
                     /inference="COORDINATES: protein motif:HMM:NF023154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HNC02623.1"
                     /translation="MNKRHPQNHNLTLHTASQWQSNPLLPRATPYIGEYLASIESVIN
                     DAVNEHPRSIAIRCDLRFPIDYNVDGTSTPISKFIASLKAQMQSADQAKHRANMRVHP
                     CTLRYVWVREWSNARLWHYHVVLFLNKDRFFTLGRFKSDPDEMAEQINLSDRIQKAWA
                     SALSISLSYAIGLVHFPQNPTYFLDCGSSDFGNQYASLYERLSYFAKEETKHYGDGKN
                     NFGCSRSRQRI"
     gene            complement(5089..5253)
                     /locus_tag="PLY05_04640"
                     /pseudo
     CDS             complement(5089..5253)
                     /locus_tag="PLY05_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(5355..6482)
                     /locus_tag="PLY05_04645"
     CDS             complement(5355..6482)
                     /locus_tag="PLY05_04645"
                     /inference="COORDINATES: protein motif:HMM:NF017843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HNC02624.1"
                     /translation="MTIITMVSVEAKNGFGNLLDKAQRQMVSITRLSQKKISNGGIQM
                     TKKWQAIDPNIAFFPVALKPIFFEPSTNTVDSEQTDLFLREFRQLPRHFAVVDESNNH
                     VFSVVTDNYKLVTNKQAFDAAQRVMQQVFKVIKPQELVCLKVTMPSTRSFCHIDLIHK
                     DADFSPWEKDKWTAFLRITNSYNRTHLLRFELGFCRWICLNGMIFGTKSVEFSYSHNK
                     QGIDKVERFIENIGDIQTLEIELTEKLHQLKRYHVPEEYMLGLACKVFEFNVPAQTDI
                     KPKRIEEMIKFRDSVNYATKKYYNEMGAHGYAALNVLTDVATRPVSAFSAESKIDGMQ
                     RKCGSWIGAFVEDISKQSFSFDAYLAEYSSSAKLLQSLVAP"
BASE COUNT         2102 a         1354 c         1319 g         1971 t
ORIGIN      
        1 taggaattag catgaaatga agaaacaaac ggcataaaaa aagcacgatg actactacag
       61 tcaccgtgct ttcacagaca taaaataagg aagttgaatt aaataaaacg gacttaagcc
      121 gacaccaaac gtaacgattc atctaaagcc cgttgtttga taatcgcccc ttgaccaaac
      181 catgctgaat ctaaacgatg gtcaatactg cgtgccttac gctgatggtc aacaaactct
      241 gtcactgaat ttaataaacc aaaggccgtc cctttagcag atgacaaatc tgaccccaac
      301 ccttgaccct caaacaaggt tttcgctttg gccatggtgc gttcaaagga ttgcatactc
      361 gcttgacctt tcatcgccgc atcaccaaac actttggcat aaaacttatc gacttcggta
      421 cttttgactt tacgttcact taaggtcttt aagcgataca taaagtcatc ccaagccgcg
      481 acagagaccc ccaattgctt cttcaccgct tgcgcatcaa aggtggtatt gtgcggaact
      541 ttcacggctt gaccttgacc atttaaggct acggctaagg tgttattaca caccacacgg
      601 atactggtaa attgagcggt tgttgctaag gttccatcac aagcggttgc taacagcaaa
      661 taaccattgg tgacatcatt accgcgcaaa ctcatggatt gacctgttct cgccaatgcc
      721 caaatcttac gaccgccttt taaaacgccc gccgtttcta attcaaagcc tgagatttcg
      781 gttaaatcac gataaaactc taggatttca cgaggctgca cgacttgata acgctgactg
      841 accaccgaca agggcgcatg ggtatccgaa cgatacaaga ccttattatc ggcaaaggta
      901 cggatagaag aggtctgctc atcacctgtc ataaaacgta caggcgcttc taaaatagac
      961 caatccatgc ctgcctgttt agcccagatt tctaagggct gttgttcttc taaatgatta
     1021 cctaaaccgt gccatggggt tgcaccgaca taagccattg tttcgactaa atgtgccatg
     1081 ataaattctc cagaataaaa tgaaatcgta cacagcacta cgccttgctc agaaagcaaa
     1141 tcggcaaaat agggcagtgt ggagtgaaaa gtgtcgtgct gctaagtaat agaaattgat
     1201 aagcagtgag ttctagaaag tagggagtaa tgtttttatt cgttgcgttt actgaaagta
     1261 tagccgcaac gtaaacactg ataattgtct aggatattgt catcgacaaa ttcaccgagt
     1321 ttagagcctg cggtgccacc tgcgacacct ccgataaaac caccgaataa cgcaccagcc
     1381 aaaccgccaa caactgagcc caatggacca gcaactgcac cgactgatgc accaattcgt
     1441 gcacctgtga aagagccaga tgcgccactg gcagcaccag ccaccgtacc gacagcacta
     1501 cctgttttac ggccatgatt gagagcgtca atctgaaaag aatgacaatg tggacattgt
     1561 agagccatga taaaagtcct gtaatagcat aagtctgata ccgccgttat tggcggtatc
     1621 agactaaagt tgagaattga aaggagtgag tgtgatgtac tcaggttagt aatatgtatt
     1681 tgaaaatttt tgggatgagg gttaaatcaa agtgcttgtg ttggcaacgc ataataatta
     1741 tgttactagc taaagttata cccttaccca aatctgtggt aactgattaa attttagttt
     1801 tgcattaatt tagcgttttc aataaaatca aagagttaag taacttgtgg gtaagatgat
     1861 gagctaaagt gtgcgcagat agatacttct atcagaaatg ttgatttgta ccctgtaaaa
     1921 ataaaactga ataaatctta atagacttat tattgctatt aaaaggtatt ttgatgataa
     1981 acaacaagtt acgaacgatt cctgttccca acaagagtga tcctgtcgca ttttttcaaa
     2041 atgcagtcaa agaggtcgta caaaatacgc aagcccctga agatatggtg cagctttgtg
     2101 cgcttgccgc catttcaacg gtcatacaac gactaattaa agtaaagtta cctggggtgg
     2161 gcgttgtgcc aacgtcactt atgttgatga tcgtggctaa atcaggtgag cgaaaatcag
     2221 ctgttgaatc aaagtttttt aatccaatta gagagtttga gcagagtaaa cggattgttt
     2281 atgaaaagga ttatcaacaa tggaaagccg aacatgaagt ctgggaaatt gagcgcaaag
     2341 ccgtacagaa aaaacttgat aaagctatta ccacaaatgc gcctagcgaa gaaattgacg
     2401 taattcgagc agaactcacc caaaaaatag ctcaagagtt aaaaaagccc acacctataa
     2461 agattttata tgatgattcg acatctcaag ctttatatag tgggctaaaa aatggattgc
     2521 tatttgctgg acttatctca agtgaaggtg gaagtgtatt aaaaagtggg gtttttagtg
     2581 atatggctaa actcaactct ctttggagtg gtgagtccat taccattgat cgcgtgaagg
     2641 ctgaaagtat tcatattagt gacgcacgct taacaacact gattatggta cagccagaaa
     2701 tacttgccga ttatatggat aaaaaaggag aacaggcacg gggatctggg ttaatagctc
     2761 gtttcctttt tagttctggg caatcaacac aagggacacg tttgatggaa gattataatc
     2821 aagagccacg ttgggaatat ttagaacgtt ttaattattt gattaaagaa tttcttgaat
     2881 caagttatca atcatcttta aaagaagcat ttaatcctca gatacttgaa ttatctccag
     2941 cagccgaaaa attatggata acttattata acaatattga aagacatata aatattgggg
     3001 gggtatatga tagttttggt gaccatgcat caaaacttgc tgagaatgca ggtagaatag
     3061 cagcgctgct gaattatttt cattggaata gcaatattat tgggagtgag gtaatggagt
     3121 atgccattaa ctgttgcgat aatttatcta agaactttct agttttcttt aacgtgcagc
     3181 ctcaagaaat acaagatgct gaaaaattat tagaatggct gaatgatact ataaaatacc
     3241 aatttcacaa gagtagaagt ataaagaaaa atgatatttt aaaatatggt ccatcttttc
     3301 taagaaaatc agaaaggctg aacaaagcac tcgaagttct attgcataat ggatcaatat
     3361 acttctttca agacatgagg cgtacaacct atgttggcct taacctatat caaatattat
     3421 aaatttgtag tgcttctagt ttgtcttata cacaaatctt cataactttc ggggctgaca
     3481 ctgacagcca atccagcaac acacgattgg gtaatcctat ttacagccta gctataactt
     3541 ctgcatgaac acggatttct tagtttaatt tttaatttca ttcattaagc ctgctatttc
     3601 tgctactagt agcagaaata gcaggcttaa agtacaatag ccgacttttt aaactcctct
     3661 agctagtgtt tttactgtcg ctgtacaacc tgtcaacaac tctctgcact actcggctta
     3721 ggcactttca taatatctac ccgtcataag ctcatcatcg acaatctacc aatgtgtgca
     3781 ataccgctct gataaactaa atgattcaaa tagaaagacc tcttggctac tataaatcca
     3841 gagttgttta ataactcagt atcaagattt tccaatagaa aatactattt tatctttatt
     3901 atgcaaaacg aagtttataa ctgtttttat tgtcggtttg gtattattaa ttaaaataat
     3961 attattaaaa tactcttact gttataacag taagagtatg atagatggtt gaaccatcta
     4021 tcatactctt taagctaaat atattagtct tagtatgtgt attagctaaa tcattaaaaa
     4081 taaacatgat gcctattgtt atgtattcaa ctaacaaggt caccatcatg aataaacgtc
     4141 atccacagaa tcataacctg actctgcata cagcctctca atggcaaagc aatccccttc
     4201 taccacgggc aacgccttat attggtgaat accttgccag tatagaatcc gtaatcaacg
     4261 atgcggttaa tgagcatccc agatcaatag cgattcgctg tgacttacgc tttccgattg
     4321 attataacgt cgatggcaca tcgactccta tctcgaagtt tatagcctct ctaaaggctc
     4381 aaatgcaatc tgctgatcaa gccaagcatc gcgccaatat gcgtgtgcat ccttgtacct
     4441 tacgttatgt ttgggtgcgt gaatggagca atgcacgatt gtggcattat catgtggtgt
     4501 tgtttttaaa caaagacagg ttctttaccc taggacgatt taaaagtgat cctgatgaga
     4561 tggccgaaca gattaacttg tctgatcgca tccaaaaagc atgggccagt gcattaagca
     4621 tatccctatc ttatgctatt ggattggttc actttcctca gaatccaacc tattttctgg
     4681 attgtggttc ttcagacttt ggaaatcaat atgcaagttt atatgaacgc ttgagttatt
     4741 tcgccaaaga agaaactaag cattacggtg atggcaagaa caactttggc tgtagccgtt
     4801 ctcgtcaacg aatatagggc tcgacacaga aaacggatta taaagtacgc taagctccgt
     4861 gaagcgtgtt gagcgtactt tttgccagaa actaccgctc gtttcgttta gcggcacaaa
     4921 tacagtgtga caaatccagt ataggaggtt tcagttatga tgaccgaacg cgcgaaggct
     4981 gctaccagaa aagccaattt gtggaatggt caggaaagca agtctatatt aagtttagta
     5041 ggctcattaa agtcttggtt taccggccgt ttgcgtttgc gctgttccca ttgaagcaac
     5101 ggcaagcctt aattttgcgg gagtcatttt ataccgtgca ccgcctttgc gtcctcgcgc
     5161 ccgagcagaa gctaaacctg cattggttcg ttcaacgatt caatcacgct catactctgc
     5221 taatgcggca aaaatcccaa agactaattt accgccaaac ccctattaga agaagaaagc
     5281 gatccagctt ttggctaata ttacattttt agcgggttgg gcaataatag ccaatatgag
     5341 attgatacaa ggcttcacgg ggcaactaaa ctttgcagca attttgcaga tgatgaatac
     5401 tctgctagat aagcgtcaaa cgaaaagctt tgtttagaga tatcttcaac aaacgcacca
     5461 atccaactgc cacattttct ttgcatccca tcaatttttg actcggcaga aaaggcgctc
     5521 acagggcgag ttgccacatc agttaacaca tttagagcag catatccgtg tgcccccatc
     5581 tcgttgtaat atttttttgt tgcataatta accgagtctc taaactttat catctcctct
     5641 attctttttg gcttgatatc tgtttgagcg ggcacattaa attcaaatac tttacacgct
     5701 aggccgagca tatactcctc tggaacatgg tagcgtttta attgatgcag cttttcggtc
     5761 agttctattt ctagagtttg aatatcgcca atattttcta taaagcgttc tactttatct
     5821 atgccttgct tattatgcga ataactaaat tcaacacttt tagtgccaaa aatcatgcca
     5881 tttaggcata tccacctaca aaaacccaat tcaaatctta aaaggtgggt tcgattataa
     5941 ctattagtta ttcgcaaaaa tgccgtccac ttatcctttt cccaaggtga aaaatcggca
     6001 tctttatgga ttaaatcaat atggcagaac gaacgtgtac ttggcatggt cactttaaga
     6061 caaacaagtt cttggggctt aatcacctta aatacttgtt gcatcacgcg ttgtgcagca
     6121 tcaaatgctt gtttgttagt cactaatttg tagttgtctg tgaccacaga aaaaacatga
     6181 ttgttgcttt cgtccaccac agcaaagtgc cgtggcagtt gcctaaactc tcttaaaaac
     6241 aaatcggttt gttcgctatc cacggtgttt gtagaaggtt caaaaaaaat tggttttaat
     6301 gcgactggaa aaaaagcgat atttggatct atcgcttgcc atttttttgt catttgtatg
     6361 cctccattgc tgattttttt ttgtgacaaa cgagtgatgg ataccatttg ccgttgagct
     6421 ttgtctaata aattaccgaa gccatttttg gcttctacag ataccattgt tataatggtc
     6481 attatcctga aagagttact agctaacatg tacatattag ctagtttgaa ggcaagtaaa
     6541 actctttttt ttggggtata agcccaaacc gccgaaaatt ccgattttag gggttaggga
     6601 gcaatggaac ggctaaccga cttagtgtga atttaaacaa acgacgcaaa agtagcgatt
     6661 ttacacgaac gtggtaacta ccccttaatg tagagagtga accaaaacgg gggctagagt
     6721 tttacgccgc cattttttcc tgctca
//
LOCUS       DAOQMB010000087         6732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4752_length_6732_cov_18.642504, whole genome shotgun sequence.
ACCESSION   DAOQMB010000087 DAOQMB010000000
VERSION     DAOQMB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_6732_cov_18.642504"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..1119
                     /gene="asd"
                     /locus_tag="PLY05_04650"
     CDS             13..1119
                     /gene="asd"
                     /locus_tag="PLY05_04650"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNC02625.1"
                     /translation="MRVGLVGWRGMVGSVLMQRMVEENDFAHFEPVYFSTSNAGGKAP
                     NFGGKEAPALLDANDVDALKGMDVILTCQGGDYTNDIYPKLRAAGWNGYWIDAASALR
                     MEDDAIIVLDPVNLNVIKDGLAKGIKNFIGGNCTNSIMLMGMGGLFHAGLVEWVSSMT
                     YQAASGGGANHMRELLKGMGVIHSAVEAELATPSSAILEIDKKVAQSIRQDVPTEFFG
                     APLAGGLIPWIDKQLDNGQSKEEWKGQAEVNKILGNTDVVPVDGLCVRIGAMRCHSLA
                     LTLKLKKDLPLDEIEAIIKSGNPWVKWVANNREISVKELTPASITGKLDIGVGRVRKL
                     NMGGEYISAFVIGDQLLWGAAEPLRRMLRLLIEG"
     gene            1229..2233
                     /locus_tag="PLY05_04655"
     CDS             1229..2233
                     /locus_tag="PLY05_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNC02626.1"
                     /translation="MKALVLNSKEEGIVFNDNYPQPAAKTGEVCIKVLAAALNHRDVW
                     ITQGQYAGIKFPTILGSDGVGVVESVGEGVDSSWLGKTVVINPNNQWGDSQRHQSKQY
                     HIMGLPKDGTLAEFVTVDADKIRLAPSHLTIEQAAALPLAGLTAYRALFGRAQLQAGE
                     KVLISGIGGGVALFAMQFAIAAGAEVYVTSSSEEKIAKAVQLGAKAGVLYNQEGWQKA
                     FVKTYGEVDVVIDSACGSGFAALIDVLGFGGRIAFYGGGQGAINGLNPQKIFWKQVSI
                     LGSTMGSDQQFVEMVDFVEKHQIKPIVDSIYALEDGVAAFKHMADGAQFGKIVVRTVA
                     "
     gene            2471..5176
                     /locus_tag="PLY05_04660"
     CDS             2471..5176
                     /locus_tag="PLY05_04660"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HNC02627.1"
                     /translation="MPGLTHALALRDVKTKSALGEPFRAELELSDLGDLSDEDIKVSL
                     AQPEDFERLGIEQTFLLAELRFEVVVNREGRSFIKISSPKRVTEPFLDFIIRVTWPNN
                     ARLHQVTALLDPPASKLTRKAEPIQAPVVVKAEPVIQPVVKTVEQAIPVVSEPVAMTV
                     TEKSYTVRKFDTLWKIARKVRPNNTVPVAQMMKALHKANPEAFIANNMNLLKDGEVLR
                     VPNLSDVQSAAQVAQQQANTPAVVQAQTTKALARQQIDATKKEKVIANTPTVAKPQMK
                     LLAPTGQKAAQSTGLTEKIAKKEQTDAPTVVTSKKVAVEKAVKKTESYNQKLTHELSS
                     LEQQLKLNDKKIAMQNAKLAQLEAQLKARRLAAEQAKHKDKTDLEKKAVATMACAVAL
                     QSFVSVDAHAADAPAKSGDSSMMPIIGGVVLLLVVAAIFVLKGKGKKETPPSTKPVAP
                     TPPVAKPTAPQPVAPKPEPVVAKPEPVKPVEPPKPQDPLEEVRPYLEMERFPQAVGIL
                     TKALVQHPDRADIHIKLLEIFVKQKDRQAFDEQYAKLEVLGDLDAIVAADKLKTQLPE
                     LPKKEEKSKLNEDGHLEFQPSKPAAVVEETPSISLDDLENDFKMSLSQPNLKALDIPL
                     AADPIELQQPEPVAAKEEAKIAELDDLLGGGLEFKFDKEPAPVAPQEEHAGLDFKLDD
                     VKFEVAEEVKPNVGTGLSLDSLDDFLAEHKIETPVEPKAPVADTSLDFESELAKFKEE
                     KQEVDTTENLVEGFELEEFKLADEPSTKENDLEFDLSADFSLDEQPVVEEKTDFNLDF
                     DTPSVDLDDLAAKFDKQALADVDLPDEDLDFDVSVSSDDVVAELDKEFSFLATTDENA
                     TRLELARAYLEMGDRLGARDLLEEVVADGNGAQKTEAQGMLMRIS"
     gene            5241..6029
                     /gene="truA"
                     /locus_tag="PLY05_04665"
     CDS             5241..6029
                     /gene="truA"
                     /locus_tag="PLY05_04665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004958810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNC02628.1"
                     /translation="MRFALGIDYEGTHYRGWQTQQEGVPSIQSTIEDVLSRIADHPVI
                     IHAAGRTDAGVHASGMVAHFDTHAIRGHKSWLLGSNTWLPKDIALRWIHPVSEDFHAR
                     FKAVARRYRYIIFNSGQRSSQLAGKVTWHYHPLNFDRMKIAASYLVGQHNFNSFRAVG
                     CQSHNPNRHVHFLNLTKRGSLIILDIQADGFLHHMVRNIAGVLMAIGEGKAEPEWVQE
                     LLRVQDRTQGGVTASGSGLYFVDALYPPEFELPKEPLGPLWLVD"
     gene            complement(5992..>6732)
                     /locus_tag="PLY05_04670"
     CDS             complement(5992..>6732)
                     /locus_tag="PLY05_04670"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005032738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNC02629.1"
                     /translation="YTPTPLSLNTEIELPEEAAHHFIKVLRARVGDKVCLFNGEGGEY
                     HGQLSRADKRHAYVTLETFIENNKSSPLKVHLGQVLSKGDRFDYAIQKATELGVSEIT
                     PLISERCEMRLGNERLDKKMAHWQGVAISACEQSGLNIVPKINSPQTIETWTANTQAD
                     LCFVLAPSVTGLPNPSQKPQTIAVLIGAEGGLSEREINQSMEQGFAPWCLGQRVLRTE
                     TAPVAVLSVLNWLWGDFNPPTTTALKAP"
BASE COUNT         2036 a         1178 c         1530 g         1988 t
ORIGIN      
        1 gaggaaaaca aaatgcgtgt aggtttagtg ggttggcgtg gcatggttgg ttctgtgctc
       61 atgcaacgta tggttgaaga gaacgatttt gctcattttg agccagttta tttttctacc
      121 agtaatgctg gcggcaaagc gcctaatttt ggtggtaaag aagcacctgc attattagat
      181 gctaatgatg ttgatgcctt aaaaggcatg gatgtgattt tgacttgtca aggtggtgat
      241 tacaccaatg acatctatcc aaaattacgt gctgcgggtt ggaatggtta ctggattgat
      301 gcagcttctg ctttacgcat ggaagatgac gcaatcatcg tgttagatcc cgttaactta
      361 aatgtgatta aagatggttt agccaaaggc atcaaaaact tcattggtgg caactgtact
      421 aactccatta tgttaatggg tatgggcggt ttattccatg caggtttggt ggagtgggtc
      481 agttcgatga cttaccaagc agcgtctggt ggcggtgcta accatatgcg tgaattatta
      541 aaaggcatgg gcgtgattca ctcagcggtt gaagcagagt tagccacacc ctcttctgcc
      601 attttagaaa ttgacaaaaa agtcgctcag tctattcgtc aagacgtacc cacagagttt
      661 tttggtgcac cattagcagg tggtttaatt ccttggattg ataagcaatt agacaatggt
      721 caatctaaag aagaatggaa aggccaagcc gaagttaata aaatcttagg caataccgat
      781 gttgtgcctg ttgatggttt atgtgtgcgt attggtgcaa tgcgttgcca cagcttagcc
      841 ttaaccctaa aactgaaaaa agatttacct ttggatgaaa tcgaagcaat cattaaatca
      901 ggtaatccat gggttaaatg ggtagcaaat aatcgcgaaa tttcggtcaa agaattaaca
      961 ccagcttcta ttactggcaa attagatatc ggtgttggtc gtgtgcgtaa attaaacatg
     1021 ggcggtgagt atatttctgc ttttgttatt ggcgaccaat tattatgggg tgctgctgag
     1081 ccattacgcc gtatgttacg tttgttaatt gaaggctaat tgtcttaaaa taaaaaaagg
     1141 cgcaaattaa tttgcgcctt ttttattttc ttaagcaaat gaatatggta aaaaagccag
     1201 tatttttaat aattatttag gaaataaaat gaaagctctt gtactaaact ctaaagaaga
     1261 aggtattgtt tttaatgata attatccaca gccagcagcc aaaacaggcg aagtatgcat
     1321 taaagtatta gccgccgcat taaatcatcg agatgtgtgg attactcaag gtcaatatgc
     1381 gggtattaaa tttccaacaa ttttaggctc tgatggtgtt ggtgttgttg agtcagttgg
     1441 cgaaggtgtt gatagctctt ggctaggtaa aacggtggta attaacccca ataatcaatg
     1501 gggtgatagt caacgtcacc aatctaagca atatcatatt atgggtttac caaaagatgg
     1561 cacgttggcc gaatttgtta ctgttgatgc tgataaaatt cgtttagcac cgagtcattt
     1621 aaccattgag caagccgctg ctttaccttt agcaggttta acggcctatc gggctttatt
     1681 tggccgtgca caattacaag caggtgaaaa agtcttaatt agtggtattg gtggcggtgt
     1741 cgcattattt gccatgcaat ttgctattgc cgcaggtgct gaagtgtatg tgacctctag
     1801 tagcgaagaa aaaattgcta aagcagtgca gttaggtgcc aaagcagggg tgttatataa
     1861 ccaagaaggc tggcaaaaag cctttgtaaa aacttatggc gaagtggatg tggtgattga
     1921 tagtgcttgt ggtagtggtt ttgcggcatt aattgatgtg ttgggttttg gtgggcgtat
     1981 tgctttttat ggtggtggcc aaggggcgat taatggttta aatccacaaa aaatcttttg
     2041 gaagcaagtt tcaattctcg gttcaacaat gggcagcgac caacaatttg tggaaatggt
     2101 tgattttgtc gaaaaacatc aaattaaacc gattgttgat agtatttatg cgctagaaga
     2161 tggagttgct gcatttaaac atatggcaga tggcgcacaa tttggtaaga tagttgttcg
     2221 taccgttgct taataacaga ataagcagac agattcgcaa taaactgctt tagttattgc
     2281 gagttaataa ttgcttgtga taaaaagaca ataatccata cttacgtcat taatgtgaaa
     2341 aaagagtaag ttattaacca catcttatcg ttgtaacaac ataagagtaa cgtgaaaaaa
     2401 taatcagcaa aggaaaaata taatggttct gcgtaagcta gctatggcac tgttgagtat
     2461 tggggtcata ttgccagggt taacccatgc tttagcccta cgagatgtca aaacaaaatc
     2521 tgcgttaggt gagccatttc gtgctgaatt agaattatca gacttaggtg atttatctga
     2581 tgaagatatt aaagtcagcc tagcacaacc agaagatttt gagcgtttag gaattgaaca
     2641 aactttttta ttggcagaat tgcgctttga agttgttgtc aatcgtgaag ggcgttcgtt
     2701 tattaaaatc tctagtccta aacgtgtcac tgaaccattt ttagatttca ttattcgtgt
     2761 cacatggcca aataatgctc gtttacatca agtaacggct ttgttagatc cgccagcaag
     2821 caagttgact cgtaaagctg aacctattca agcccctgtt gtggtaaaag ctgagcctgt
     2881 gattcagcca gttgttaaaa cagttgagca agcaatacct gtagtctcag aacctgtcgc
     2941 tatgaccgtg actgaaaaat catacacagt acgtaaattt gataccttat ggaaaattgc
     3001 tcgcaaagtt agacccaata ataccgttcc cgttgcacag atgatgaaag cattacataa
     3061 ggccaatcca gaggctttta ttgctaataa tatgaatctg ttaaaagatg gtgaagtatt
     3121 gcgtgtacct aatttaagtg atgtgcaatc agcagcacaa gttgcacaac aacaagctaa
     3181 tacacctgct gtagtacaag cacaaactac taaagcttta gcacgacagc aaattgatgc
     3241 aactaaaaaa gaaaaagtta ttgcaaatac accaactgtt gccaaaccac aaatgaaatt
     3301 gttggcacca actgggcaaa aagcggctca aagtacaggc ttaacagaaa aaatagctaa
     3361 aaaagagcaa actgatgcac caaccgtcgt gactagcaaa aaggttgctg ttgaaaaagc
     3421 ggtgaaaaaa acggaaagtt ataaccaaaa actaacacat gagttaagtt ccttagagca
     3481 acaactcaag ttgaatgata agaagattgc tatgcaaaat gccaaattag cgcaattaga
     3541 agcgcagtta aaagcacgtc gtttagcggc tgaacaagct aaacacaaag acaaaaccga
     3601 tttagagaaa aaagctgttg ctacaatggc ctgtgcagtt gccctgcaga gttttgttag
     3661 tgtagatgcc catgctgctg atgcacctgc aaagtctggt gatagttcaa tgatgccgat
     3721 tattggcgga gtggtattac tattagttgt tgcagctatt tttgttttaa aaggtaaggg
     3781 taaaaaagaa acaccaccat ccactaaacc tgttgcacca actccacctg ttgcaaagcc
     3841 tacagcccct cagcctgttg caccaaaacc tgagcctgta gttgctaaac ctgagcctgt
     3901 taaacctgtt gagccaccaa aaccacaaga ccccttagaa gaagtgcgtc cttatttaga
     3961 aatggagcgt tttcctcaag cggtaggcat tctcactaag gctttagttc agcatcctga
     4021 tcgtgctgat attcatatta aattactgga aatttttgtt aagcaaaaag atcgtcaggc
     4081 ctttgatgaa caatatgcaa aattagaagt gttaggtgat ttagacgcaa tcgttgcggc
     4141 tgataaatta aaaacacaat tacctgaatt gcctaaaaaa gaagaaaaat caaaattaaa
     4201 cgaagatgga catttggagt ttcagccctc taaaccagca gctgtggttg aagaaacgcc
     4261 ttctattagt ttagatgatt tagaaaatga ctttaaaatg agtttgtctc agccaaattt
     4321 aaaggcatta gatattcctt tagctgctga tcctattgaa ttacaacaac cagagcctgt
     4381 agcagctaaa gaagaagcaa aaattgctga attagatgat ttgttgggcg gtggtttaga
     4441 gtttaaattt gataaagaac cagctcctgt tgctcctcaa gaagaacatg caggcttaga
     4501 ctttaaatta gatgatgtta aatttgaagt tgctgaagag gtcaaaccaa atgttgggac
     4561 tggtttgtct ttagatagct tggatgactt tttggctgag cataaaattg aaacgccagt
     4621 agagccaaaa gcaccagtag cagatacttc tttagacttt gaatcagaat tggctaagtt
     4681 taaagaagaa aagcaagaag ttgatacgac tgaaaacttg gtagaaggct ttgagttaga
     4741 agagtttaaa ttagctgatg aaccaagcac caaagaaaat gacttagagt ttgatttgtc
     4801 tgctgatttt tctttagacg aacaaccagt tgttgaagaa aaaacggact tcaacttaga
     4861 ttttgacacc ccttcggtcg atttggatga tttagcagcc aagtttgata aacaagcttt
     4921 agctgatgtt gatttgccag atgaagacct agactttgac gtgagcgtgt cttctgatga
     4981 tgttgtggca gaattagata aagagttttc gttcttggcg acaactgacg aaaatgcaac
     5041 tcgtttggag ttagcacgtg cttatttaga aatgggtgat cgtttaggtg cgcgtgattt
     5101 attagaggaa gtagtggccg acggtaatgg cgcgcaaaaa acagaagctc agggtatgtt
     5161 aatgcgaatt agctaataac agacttgcct tttgataaaa gccgctgtaa aaagcggctt
     5221 ttgttttttg agtatttaag atgcgttttg ctttaggaat tgactacgaa ggtactcatt
     5281 atcgtggctg gcagactcag caagaaggcg ttccgagtat tcaatcaaca attgaagatg
     5341 tattgagtcg tattgctgac catcctgtga ttattcatgc cgcggggcgt actgatgctg
     5401 gggttcatgc ttcaggcatg gttgcgcatt ttgataccca tgcaattcgt ggtcacaaaa
     5461 gttggttgtt gggttcaaat acatggttgc ccaaagatat tgctttacgc tggattcatc
     5521 ctgtcagcga ggattttcat gctcgcttta aagcggttgc tcgacgttat cgttatatta
     5581 tttttaacag tggacaacgc tctagtcagt tagcaggaaa ggtgacttgg cattatcatc
     5641 ccttaaattt tgaccgtatg aaaattgcag ccagttattt ggtaggccaa cataatttta
     5701 attcttttag ggcggtaggt tgtcaatcgc ataatcccaa tcgtcatgta cattttttaa
     5761 atttaacaaa gcgcggttca ttgattattt tagatattca ggccgatggt tttttgcatc
     5821 atatggtacg caatattgca ggtgtactca tggcgattgg tgaggggaaa gctgagccag
     5881 aatgggtaca agagttatta cgggtgcaag atcgaactca aggcggagtc acagcatcag
     5941 gcagcggttt gtattttgtc gatgccttgt atccgccaga gtttgaatta cctaaggagc
     6001 ctttagggcc gttgtggttg gtggattaaa atcaccccaa agccaattta atacgcttaa
     6061 tacggcaacg ggggcggttt ctgtgcgtaa tacccgttgt cctaagcacc atggcgcaaa
     6121 accttgttcc atgctttggt ttatttctcg ttcggataat ccgccttctg cgccaatcag
     6181 tactgcaatc gtttgtggtt tttggctcgg attgggcaaa ccagtgacac tgggtgcaag
     6241 cacaaaacat aaatcagcct gtgtgtttgc tgtccatgtt tctatggttt gagggctgtt
     6301 gattttaggc acaatattta agcccgattg ttcacaagca ctaatggcta caccttgcca
     6361 atgcgccatt tttttgtcta aacgctcatt gcccaaacgc atttcacaac gctcactgat
     6421 gagaggagtg atttcgctaa cgcctaattc agtcgctttt tgaatggcat aatcaaatcg
     6481 atcacctttg gataatactt gccctaaatg aacttttagt ggagagcttt tgttattttc
     6541 aataaaagtt tctagggtga cataagcatg gcgtttgtca gcacgactta attggccatg
     6601 atattcacca ccttcaccat taaataaaca gactttatcg ccaacgcgcg cgcgtaagac
     6661 tttaataaag tgatgagcag cttcttcagg cagttcaatt tcggtattga gcgataaggg
     6721 agtaggggtg ta
//
LOCUS       DAOQMB010000088         6690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4790_length_6690_cov_8.580708, whole genome shotgun sequence.
ACCESSION   DAOQMB010000088 DAOQMB010000000
VERSION     DAOQMB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4790_length_6690_cov_8.580708"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..789
                     /locus_tag="PLY05_04675"
     CDS             85..789
                     /locus_tag="PLY05_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC02630.1"
                     /translation="MQLLKRPGGELASRPLHFIWIADCSGSMSGDKIQQLNFAIRESI
                     PAMRGVANENPNADVLVRALKFSNGAQWHVGQATKVTDFEWIDLKADGLTDMGKALKM
                     VAEQLKMPPMAERALPPVLVLLSDGQPTDNFEEGLQALLDLPWGKKAVKIAIAIGEDA
                     DTDVLQQFMGSNSELKPLIAKNAEQLVNYIKWASTAVLKAASSPVSQTVALPQGVNVP
                     VPAPPTANISSADDVW"
     gene            795..1766
                     /locus_tag="PLY05_04680"
     CDS             795..1766
                     /locus_tag="PLY05_04680"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNC02631.1"
                     /translation="MSDYTWQVSTQSIQGASHIRQGLPNQDACAAYWTKPKQLPLIMA
                     VADGHGSKKSFRSERGARLAVEQALQVMQDFVNSQPRFCDVQWQAEEILSKRLVQYWR
                     QAVEQDVAQQPFVAEHEALLPNNKEPYLAYGSTLLIAVITDVFILLMQLGDGDILQVL
                     PCGTVQRPIMADARLLGNETTSLCTKDAWRQFRCSVQAPSNQVSSLWLLATDGYANSF
                     SSASHFEQVGTDLLLMIQQSGFDLVIQHLPEWLKAASQQGSGDDVTVALAYQQAIKPV
                     RYVLESQRKTLLPRKTSSMQQRKQIMSKRVRRNLTELSQLLTECMKK"
     gene            1763..2644
                     /locus_tag="PLY05_04685"
     CDS             1763..2644
                     /locus_tag="PLY05_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC02632.1"
                     /translation="MTDVVRQKLQTLISQFGVAICNDVKRCEALLKDLCPNHKREVNL
                     LLAALREGVAKELLNASPLVPIESTISRLILKMHDNLGITEQFAQWAVESWVLALGVQ
                     FNSSAIAPSPDKGKVGIGLPNCAESIKPIDQPSQAIIEQFKDWQKIGRSGKRLAVDAT
                     HWVAVIDKKTNLMWAVNSSKRSGFPNPNKQITWNEAHNFCKRVNHSSGWCGYHDWRLP
                     SIDELKTLLTKYKQSNLYITEEVFIDIPSTYYWVWSSSSVANDGGYACVVSFNEGNDC
                     KEYKNNDNYVRLVRSGQ"
     gene            2810..3496
                     /locus_tag="PLY05_04690"
     CDS             2810..3496
                     /locus_tag="PLY05_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC02633.1"
                     /translation="MKAIVYVDGYNFYYGCLKHSTDKWLDIYKLFSQFILKAQCPQVT
                     DIIIKFFTAPIHAKVATHGQQAQIAQQNYQRALTTLYPKQVQIISGYYTLEKAKLLAY
                     KQPPDKNDRLEVWKLEEKQTDVNIALTAYRDAIKGLADILVFVSNDTDLEPALSAIRQ
                     DMGQTIQIGIVIPLRQPEPHKPKRPPNQRLSKYANWTRDYLTHDELANSHLPLKIPTS
                     KKTIFKPDYW"
     gene            3546..4427
                     /locus_tag="PLY05_04695"
     CDS             3546..4427
                     /locus_tag="PLY05_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC02634.1"
                     /translation="MPDIPRQQLAYILTQFGRSICDDPKRCEALLKDLCPNHKREVNL
                     LLAALREGVVTELLNASPLVPIESSINRLTLKMHNNLGTDKLLAQWAVESWTMVLGIQ
                     FNSSIIVPSPDKAKVGMGLPKSAESIKLINQPSQAIINQFKGWQKIGLQGEKLAADAP
                     QWAAVIDEKTQLMWAINSSKTASFPNPNKRINWKDAMTWANYVNTQGWCGFNNWRLPT
                     IDELKTLLTQRKQPNLYIREDIFSDIPKKYYWAWSSSPFAALNSHNAWIVNFYGGYSY
                     YYDKGYERDVRLVRASQ"
     gene            4467..5768
                     /locus_tag="PLY05_04700"
     CDS             4467..5768
                     /locus_tag="PLY05_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC02635.1"
                     /translation="MHQLLKLKQTVQLSQHNITCTVEQFLGGGGQGEVYKATLNGKAV
                     ALKWYFAEQATQEQQQSIEELIKKGAPTTNFLWPIDIATAKGIKGFGYIMPLREARYK
                     SLFDLMKRRAEPTFSALITAAYQLAENFYQLHGKGLCYRDISHGNIFFDPQTGEILIC
                     DNDNVSVNGTDKTGVYGTPRFMAPEIVRAEAKPNTQTDLFSLAVLLFYMLMMHHPLEG
                     KKESDIKCFDLPAMNKLYGFEPVFIFDPQNNSNQPVVGYHDNALIYWPLYPQFLRDLF
                     VKAFTDGLNNTNNRIRESEWRFALIRLRDSIVYCQHCHSENFADAQQNQAQKICWNCQ
                     KPFSLPVKLSVNNQVIMLNANTQLFAHHLDPNRKYDFTQPLAIMQQHPQNPNIWGLKN
                     LSTTWQITTADGTVKVVEPQRSVALNLGTIIDFGIVEGHFV"
     gene            5785..6555
                     /locus_tag="PLY05_04705"
     CDS             5785..6555
                     /locus_tag="PLY05_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HNC02636.1"
                     /translation="MNKFDAPISQQEIDHLAAFLAQDFGYKTFDIDQLRGFLWAVAGS
                     PVVVEPNELLEVIFNIDENEKPPANYTPFKNPTQAKDYLSLILRLHDEAIEQIIDEEF
                     VWPTEYCIDVDGQESYNFRQWCSGFIYGYVWLEETWDDCLQQLVGDIAQDEDEQELFK
                     QQFKLLAFALTYLSADAEGKQHFLDQFANSEGDSISHDKMPTVQDIQGQLLAYGILGY
                     QIRQMVEPSSEPVRVEKIGRNDPCLCGSGKKYKKCCGA"
     gene            6564..>6690
                     /locus_tag="PLY05_04710"
     CDS             6564..>6690
                     /locus_tag="PLY05_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNC02637.1"
                     /translation="MYLSPIHLRQAVNTLKSGGVIAYPTEAVWGLGCDPFNEQAVL"
BASE COUNT         2128 a         1212 c         1433 g         1917 t
ORIGIN      
        1 taatggagtt ggaattagca ggtaaaattg ctactgtagc aggtggttat gagcaattga
       61 gttaacaaaa cataaggaaa aaccatgcaa ttattaaaaa gacctggcgg tgaattagcc
      121 tcacgccctt tacattttat ttggattgcc gattgttcgg ggtcaatgtc gggtgacaaa
      181 attcagcagt taaattttgc tattcgggag tctattcctg caatgcgtgg tgtagctaat
      241 gaaaatccca atgcggatgt tttagtccga gccttaaagt tttctaatgg cgcacaatgg
      301 catgttggcc aagcaaccaa agtaaccgat tttgaatgga tagatttaaa agccgatggt
      361 ttgactgata tgggcaaagc cttaaaaatg gttgccgaac agttaaaaat gccgcctatg
      421 gctgagcgtg ctttgccgcc tgtgttagtg ttgctgtcag atggccaacc taccgataat
      481 tttgaggaag gtttacaagc attattagat ttaccgtggg gcaaaaaagc ggtcaaaatt
      541 gcgattgcga ttggtgaaga tgccgataca gatgtattac agcaatttat gggcagcaat
      601 agcgaattaa agccgttaat tgcgaaaaat gccgagcagt tagtgaatta tattaaatgg
      661 gcatcaactg cggtattaaa agcagcttcg tcacctgtaa gccaaacagt cgccttgccg
      721 caaggtgtta atgtacctgt tccagctcca cctacggcca atatcagttc tgctgatgat
      781 gtgtggtaac aataatgagc gattacacat ggcaagtgag tacccaaagt attcagggag
      841 caagccatat tcggcaaggt ttgcccaatc aagatgcgtg tgccgcttat tggactaaac
      901 ctaagcagtt gccgctgatt atggcggtag ccgatggaca tggcagtaaa aagagtttta
      961 gaagtgagcg tggcgcacgt ttggccgtag aacaagcctt acaagtcatg caggattttg
     1021 taaatagtca gccgcgtttt tgtgatgtgc aatggcaagc cgaagagata ttaagtaaac
     1081 gcttagtgca atattggcga caagccgtag aacaagatgt ggctcaacag ccttttgtag
     1141 ccgagcatga agccttgttg ccgaacaata aagagccata tttagcctat ggttctactt
     1201 tgttaattgc ggtaatcact gatgttttta ttttgctgat gcaattgggt gatggcgata
     1261 tattgcaagt attgccgtgt gggacggtac aacgacccat aatggcagat gcgcgtttat
     1321 tgggtaacga aacaacctct ttatgcacca aagacgcatg gcggcaattt agatgcagtg
     1381 ttcaagcacc gtctaatcaa gtatctagtt tgtggttgtt agcaaccgat ggttatgcca
     1441 actcttttag cagtgccagt cattttgagc aggtaggtac ggatttgttg ttgatgattc
     1501 aacaatcagg ttttgacttg gtaattcagc atttacctga atggcttaaa gccgcctcgc
     1561 aacagggcag tggtgatgat gtgacggtgg ctttggcgta tcagcaagct attaagcctg
     1621 tacgttatgt tttagaaagt cagcgtaaga cgttgttgcc tcgcaaaaca tcttctatgc
     1681 agcaacgtaa acaaataatg tctaaacgcg tacggcgtaa tttgaccgag ctatcgcagt
     1741 tattaacgga gtgtatgaaa aaatgaccga tgtggtgaga caaaaattac agactttgat
     1801 aagtcaattt ggcgttgcta tatgtaatga tgtcaaacgg tgtgaggcgt tattaaaaga
     1861 tttatgcccc aatcataaac gtgaagtgaa tttgttgctt gctgctttgc gagagggtgt
     1921 ggctaaagag ttgttaaatg cttctccttt agtgcctatc gaaagtacca taagccgttt
     1981 aatcttaaaa atgcacgata atttaggcat tactgaacag tttgcacaat gggcggtaga
     2041 aagttgggta ttagcgttag gtgttcaatt taacagcagt gcaattgctc cttcccctga
     2101 taaaggaaaa gttgggatag ggttgccaaa ctgtgctgag tcaataaagc ccatcgatca
     2161 accctctcaa gccattattg agcagtttaa agattggcaa aaaataggta gaagtggcaa
     2221 aaggttagcg gtagatgcga cacattgggt agcagtgatt gataaaaaaa cgaatctgat
     2281 gtgggcggta aactcaagta aaagatcagg ttttcctaat ccgaataaac aaataacatg
     2341 gaatgaggct cataatttct gcaaacgtgt taatcattca tcgggttggt gtggctatca
     2401 tgattggcga ttacctagca ttgatgaatt aaaaactcta cttactaaat ataaacaatc
     2461 taatttgtac atcacggaag aggtttttat tgatattcca tctacatatt attgggtatg
     2521 gtcttcctct tctgttgcta atgatggtgg ctacgcgtgt gttgtcagtt tcaatgaagg
     2581 caacgattgt aaagaatata aaaacaatga caattatgtg cgccttgtgc gttcgggtca
     2641 gtaacttagc gttcttacaa aataacatca tataaatttg ccaatagggt aaataatttt
     2701 gtattatcac tctacaactc atcctttgcg gagttattta gttagccact gcttaggacg
     2761 aaaaaaccgc tttttggcgg ttttttcatt tctaagggat taaactgtta tgaaagcaat
     2821 agtatatgta gatggttata atttttatta tggttgttta aaacattcaa cagacaaatg
     2881 gcttgatatt tacaagttat ttagccaatt tatattaaag gcgcaatgcc cacaagttac
     2941 tgatattatt attaaatttt ttactgcacc tattcatgcc aaagttgcaa cacatggaca
     3001 acaagcacaa attgctcaac aaaactatca aagagcatta accaccttgt acccaaaaca
     3061 agtacaaatt attagtggtt attacacttt agaaaaagca aaactccttg cgtataaaca
     3121 accgcccgat aaaaacgata gactagaagt atggaagcta gaagaaaagc agacagatgt
     3181 gaatattgca ttaactgctt atcgagatgc tataaaagga cttgcagata tattagtgtt
     3241 tgtttcaaat gatacagacc tagaacctgc attgagtgcc attcgccaag acatgggaca
     3301 aaccatacaa ataggtattg taattccgct gagacagcca gaaccacata aacctaaacg
     3361 gccacctaat caaaggctta gtaaatatgc aaactggaca agagactatt tgacccatga
     3421 tgaattggca aactctcatt taccattaaa aataccaaca tctaaaaaaa cgatttttaa
     3481 acctgattat tggtaagaaa gttgtttaga ataattgaaa tatgtactaa ataaagaaac
     3541 aacccatgcc cgatattcca cgccaacaac ttgcttatat tttgacgcaa tttggtcgta
     3601 gtatttgtga cgaccctaag cgttgcgagg cactattaaa agatttatgc cctaatcaca
     3661 aacgtgaagt gaatttgttg cttgccgctt tgcgagaagg cgttgtaaca gagttgttaa
     3721 atgcctcacc cttagtgcct atagaaagca gcattaaccg cttaacctta aaaatgcaca
     3781 ataatttagg cacagacaaa ctcttggcac aatgggcagt agaaagttgg acgatggttt
     3841 taggcattca atttaatagc agtataattg tgccttctcc tgataaggca aaggttggaa
     3901 tggggttgcc gaaaagtgct gagtcaataa aactcatcaa tcaaccctct caagctatta
     3961 ttaaccaatt taaaggttgg caaaaaatag gtttacaggg tgaaaaatta gccgcagatg
     4021 cgccacaatg ggcggcggtg attgacgaaa aaacacaatt aatgtgggca ataaattcaa
     4081 gtaaaacggc gagttttcct aatcctaata aaagaataaa ttggaaagac gcgatgacat
     4141 gggcaaatta tgtgaatacg caaggttggt gtggttttaa taattggcga ttacccacta
     4201 ttgatgaatt aaaaacactg ctaactcaaa gaaaacaacc gaatttgtat atcagagaag
     4261 atatttttag cgatattcca aaaaaatatt attgggcatg gtcttcctcg ccttttgccg
     4321 ccctcaatag tcacaacgcg tggattgtca acttctacgg cggctactct tactactacg
     4381 ataagggcta tgagcgcgat gtacggcttg ttcgcgccag tcaataattt ggtattttgg
     4441 ctttttaact cttaaaggaa taaaaaatgc accaactcct aaaactcaaa caaacagtac
     4501 aacttagcca acataacatc acttgcaccg tagagcaatt tttaggtggt ggtggtcaag
     4561 gtgaagtgta taaagccaca cttaacggca aagccgtggc tttaaagtgg tattttgccg
     4621 agcaagctac ccaagaacaa caacaaagta ttgaagaact gattaaaaaa ggcgcaccca
     4681 ccaccaattt tttatggcca atagatatag ccactgccaa aggcataaaa ggctttggtt
     4741 acattatgcc cctgcgtgaa gcgcgttata aaagtttgtt tgacttaatg aaaagacgag
     4801 ccgaacctac atttagcgca ttgattacag ccgcttatca acttgccgaa aacttttatc
     4861 aactgcatgg taaaggattg tgttatcgcg atatatcgca tggcaatatc ttttttgacc
     4921 ctcaaacggg cgaaatatta atttgtgata acgataatgt cagcgtcaac ggcacagata
     4981 aaacaggtgt ttatggcaca ccacgtttta tggctcccga aattgtccga gccgaagcta
     5041 aacctaatac gcaaacagat ttattttctt tagcggtatt gttgttttat atgctgatga
     5101 tgcaccaccc tttagaaggc aaaaaagaat ctgacatcaa atgttttgat ttacctgcca
     5161 tgaataaact ctatggtttt gagcctgtat ttatttttga cccgcaaaat aatagcaatc
     5221 aacctgttgt aggctaccat gataacgcct tgatttattg gccattgtat ccacaatttt
     5281 tgcgtgattt atttgtcaaa gcctttactg acggattaaa taataccaat aatcgtataa
     5341 gagaaagtga atggcgattt gcactgattc gcttgcgtga cagtattgtg tattgtcaac
     5401 attgccatag cgaaaacttt gccgatgcac aacaaaatca agctcaaaaa atatgttgga
     5461 attgtcaaaa gccctttagt ttgcctgtaa agttgagtgt gaataatcag gttattatgc
     5521 taaatgccaa tacgcagtta tttgcacatc atttagaccc aaaccgtaag tatgatttta
     5581 cgcaaccttt agcaatcatg cagcaacacc cacaaaatcc gaatatttgg ggcttaaaaa
     5641 acctttcaac aacatggcaa attacaacgg ctgatggcac ggttaaagtc gtagagcctc
     5701 aacgtagtgt ggcgttgaac ttaggcacaa tcattgattt tggcatagtt gaaggccatt
     5761 ttgtttaaat aggacgaaat gataatgaat aaatttgatg cacccatttc tcaacaagag
     5821 attgaccatt tggccgcttt tttagcacaa gattttggct ataaaacctt tgatattgac
     5881 cagttacgcg ggtttttgtg ggcagtggct ggtagtcctg ttgttgttga gcctaatgaa
     5941 ttgctcgaag ttatatttaa tatagatgaa aacgaaaaac caccagccaa ctatacgcct
     6001 tttaaaaacc ctacacaggc taaggattat ttaagcctta ttttgcggct acacgatgag
     6061 gcaattgagc aaattataga tgaagagttt gtatggccaa cagaatactg tattgatgtt
     6121 gatggccaag aaagttacaa ctttcgtcaa tggtgtagtg gatttatata cggttatgta
     6181 tggctagaag aaacatggga tgattgttta caacaactcg tgggtgatat tgcccaagat
     6241 gaagacgagc aagagttatt taagcagcaa tttaaactat tggcatttgc tttaacgtat
     6301 ttatctgctg atgcagaggg taaacaacac tttcttgatc aatttgcaaa cagtgaaggc
     6361 gactccatta gccatgataa aatgccaacc gtacaggaca ttcaagggca gttattagcg
     6421 tatggcatat taggttatca aatacgccaa atggttgagc caagttcaga acctgtacga
     6481 gtggaaaaaa taggccgtaa tgacccttgc ctttgtggta gtggcaaaaa atacaaaaag
     6541 tgttgtggag cataacaaaa cttatgtacc tatctccgat tcatcttcga caagctgtaa
     6601 atactttaaa atcaggtggt gtgattgcct atccaaccga agccgtttgg ggcttggggt
     6661 gtgacccgtt taatgaacag gcggtgcttg
//
LOCUS       DAOQMB010000089         6535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_4966_length_6535_cov_16.286728, whole genome shotgun sequence.
ACCESSION   DAOQMB010000089 DAOQMB010000000
VERSION     DAOQMB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_6535_cov_16.286728"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1469)
                     /gene="nqrF"
                     /locus_tag="PLY05_04715"
     CDS             complement(231..1469)
                     /gene="nqrF"
                     /locus_tag="PLY05_04715"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HNC02638.1"
                     /translation="MSQEAVIILSMVMFTVTVLVLVAFILAARSKLVSEGTVTIDINH
                     DPEKRLEVPSGGKLLQCLAEQKLFLSSACGGGGTCGQCKVKVKAGGGDILPTEEPHFT
                     KGEIREGWRLGCQLAVKQNLEIELDEEFFGVKRWECEVISNDNVATFIKELVLKLPEG
                     EDVHFRAGGYMQLEAPAYENSYKDFDIQPEYRGDWEKFKLFDVKAKTTEPTIRAYSMA
                     NYPEEKGLLKFNIRIATPPLRQLHEVPAGKMSSWVFNLKKGDKVTVFGPFGEFFAKET
                     KAEMVFIGGGAGMAPMRSHIFDQLKRIKTDRKISFWYGARSLRETFYADEYDQLAKDN
                     PNFEWHLALSEPQPEDNWTGYTGFIHNVLYENYLKNHQAPEDCEFYMCGPPMMNAAVI
                     KMLLDLGVDRENIMLDDFGG"
     gene            complement(1480..2097)
                     /gene="nqrE"
                     /locus_tag="PLY05_04720"
     CDS             complement(1480..2097)
                     /gene="nqrE"
                     /locus_tag="PLY05_04720"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HNC02639.1"
                     /translation="MEHYLSLFTKAVFIENMALSFFLGMCTFLAISKKIQTAISLGIA
                     VVFVQTLTVPANNLLWQYLLKEGALEWTGMQGASSVDLSFLSLMSYICTVAALVQVIE
                     MACDKYLPALYNALGIYLPLITVNCAVLGGALFMQQRDYNFSESVVYGLGSGGGWALA
                     IVLIAAVREKLKYSDVPAGLQGLGITFISAGLMALGFMSFSGIKL"
     gene            complement(2109..2780)
                     /locus_tag="PLY05_04725"
     CDS             complement(2109..2780)
                     /locus_tag="PLY05_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HNC02640.1"
                     /translation="MAEQQSTKEILLGPILNTNPVIFQVLGICSALAVTSTMKNALAM
                     SIGLTLVTAFSNFFISMIRNVIPSSIRIIVQMVVVATLVIIVDQLLQAFVPEIAKQMS
                     VFVGLIITNCIVMGRLEAFAMKNPPVISFVDGFGNGLGYSALLMLLAFFRELFGSGTV
                     FGFTIMQTVNNGGWYIPNGLLLLPPSAFFLIGLTIWALRSWKTDQQESTEFKIAPHSK
                     AQTHH"
     gene            complement(2782..3567)
                     /locus_tag="PLY05_04730"
     CDS             complement(2782..3567)
                     /locus_tag="PLY05_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HNC02641.1"
                     /translation="MSKESTGKTLVVASVLSVVCSIALAAAVTLLKPIQDENKALEKQ
                     RKILAAAQVLPEGEGDKARVNQEFAKFQRYLVNLDTGEFREVKLDDPYDQRKALKNSS
                     LSMALTADQDMAGLRRRAKEADIYVVRDAAGKIEKMVLPVSGYGLWSTLYGYLVLKND
                     ANTIMGVTFVEHAETPGLGGEVDNPKWKALWINKQVYSADGQTHFKIIKGNVDPAKPD
                     AIHQVDGLSGATLTSNGVTNLVQFWMGDLGYKKFLSKVREGGI"
     gene            complement(3560..4837)
                     /locus_tag="PLY05_04735"
     CDS             complement(3560..4837)
                     /locus_tag="PLY05_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003658466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HNC02642.1"
                     /translation="MKWMRNKLDNMAPHFEKGGRYEKWYPLYEAVDTFLYSPPNVTHA
                     GSHVRDSVDLKRIMILVWMATFPAMFFGMWNVGNQALGTLAYAMQHNPAVTPELAIDG
                     WRHWLIALLAGFDANSWWDCLVYGATWFVPIYAVTFIVGGFWEVLFAIKRGHEVNEGF
                     FVTSVLFALVCPPTVPLWQVALGISFGVVIGKEVFGGTGKNFINPALAGRAFLFFAYP
                     AYMSGDTVWTAISPEVWKLGVDGFSGATALGQAKLAAYTGVQHMTATGLQPLSWWDAF
                     VGFEAGSVGETSTLAILIGAAILLIARVASWRIMAGVMIGMATMSFIFNQIGSDTNPM
                     FAMPWHWHLVLGGFAFGMVFMATDPVSAAMTNTGRLVFGIVIGVMVVLVRVVNPAYPE
                     GMMLAILFANLCAPLIDYFVMQANIKRRALRNV"
     gene            complement(4844..6178)
                     /locus_tag="PLY05_04740"
     CDS             complement(4844..6178)
                     /locus_tag="PLY05_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HNC02643.1"
                     /translation="MVKIKRGLDIPITGAPSQQISDMSSVRSVAILGADYMGMKPTMK
                     VAEGDRVVKGQPLFADKKNPQVIFTAPVTGRVRAINRGAKRVFQSLVIDCESNSSEQI
                     TFRQYNRDQLANLNRDGVIEQLLASGLWTAIRTRPFSKIPDPTTTPSAIFVNAMDTNP
                     LAADPEVVIKQQQEAFEDGLALVSKLADKLYVCKAEKAQFSTGKFNAHEFSGAHPAGL
                     VGTHIHFLHPASASSVVWHLNYQDVIAIGKLFATGELDSTRIIALAGASVKQPRLAKT
                     IVGASLLDMTTGELSGSDNRIISGSVLSGRHAQGVEAYLGRYHLQVSVIPEAKEREML
                     HYMKLGSHRFSALGVYLGRWLGKKFNMTTSSNGSPRAMVPVGIYEDIMPLDILPTQLL
                     RYLLVGDTDMAQSLGCLELDEEDLALCTFVCPGKYEYGPILRDVLDRIEKEG"
BASE COUNT         2076 a         1554 c         1137 g         1768 t
ORIGIN      
        1 cgcccaaggc atactataat aagcaacgcc cgaaaaactg acaggctctt tggcaacggt
       61 tgaacaactc gcaacactga tactgaataa agcaatcaac aaactacgca taacaaatct
      121 aacaagaaaa attttgtcga agtatcaaag gatgtttaat gaaactcaag actaaatttg
      181 ttgtaaaaaa taaaggcgca tttctgcgcc tttattttaa gaaactcagt ttaaccacca
      241 aaatcatcca acatgatatt ttcgcggtca acacccaagt ctaataacat tttaatcacc
      301 gctgcgttca tcatcggtgg gccacacata taaaactcac aatcttcggg agcttggtgg
      361 tttttaagat aattttcata taaaacgtta tgaataaaac ccgtataacc tgtccaatta
      421 tcttcaggtt gtggctctga taaggccaaa tgccattcaa aattaggatt atctttggct
      481 aattgatcgt attcatcggc ataaaaagtt tcgcgtaatg aacgcgcacc ataccaaaaa
      541 ctgattttac ggtcggtttt gatacgtttt aattggtcaa aaatatgcga acgcatcggt
      601 gccatacctg caccaccacc aataaacacc atctcggctt tagtttcttt ggcaaaaaat
      661 tcaccaaaag gcccaaacac agtaactttg tcgcctttct ttaagttaaa tacccaagaa
      721 gacattttac ctgctggcac ttcgtgtaat tgacgcaaag gtggcgtagc aatacgaata
      781 ttaaacttta acaagccttt ttcttcgggg taattggcca ttgagtaagc acgaatcgta
      841 ggttcggtgg ttttggcttt aacatcaaac aacttaaact tttcccaatc gccacggtat
      901 tcaggctgaa tatcaaagtc tttgtagctg ttttcgtagg caggggcttc gagctgcata
      961 taaccgcctg cgcggaaatg cacatcttca ccttcgggta gttttaatac caactcttta
     1021 ataaaagtcg ctacgttatc gttagaaata acttcacatt cccaacgctt aacaccaaaa
     1081 aactcttcat ctaattcgat ttcgagattt tgcttaaccg ctaactgaca acccaaacgc
     1141 caaccttcac gaatttcgcc tttggtaaag tgtggctctt cggtgggtaa aatatcacca
     1201 ccacctgctt taacttttac tttacattga ccacacgtac cgccaccacc acaggcagac
     1261 gacaaaaaca atttttgttc ggccaaacat tgtaataatt taccaccact tggcacttct
     1321 aaacgttttt cggggtcgtg gttaatatca atagtgactg tgccttcgga cactaatttg
     1381 gagcgtgctg ctaaaataaa cgcaaccaac accaataccg tcaccgtaaa catgaccata
     1441 ctgaggataa taactgcctc ttgagacatt tccctctcct tataacttga tgccagagaa
     1501 ggacataaag cctaaagcca tcagtcctgc cgaaataaaa gtaatcccta agccctgtaa
     1561 gcctgctggc acatcgctat attttaattt ttcgcgcaca gcggcaatca aaacaatcgc
     1621 caatgcccag ccaccaccag aacctaaacc ataaaccaca ctttcgctaa agttgtagtc
     1681 acgttgctgc atgaataatg caccacccaa taccgcacaa ttaacggtaa ttaacggcaa
     1741 atagatgcct aatgcgttat acaacgcagg caagtattta tcacaagcca tctcaatgac
     1801 ttgcacaagc gcggcaacag tacagatata actcatcaaa cttaaaaagg acaaatcaac
     1861 agatgatgcg ccttgcatcc ccgtccattc taatgcacct tctttgagta aatattgcca
     1921 caataaattg ttagcaggta cggtcaaagt ttgcacaaaa accaccgcaa tccccaaaga
     1981 aatcgcggtt tggatttttt tggaaattgc taaaaaggta cacataccca agaagaaaga
     2041 cagtgccatg ttttcaataa acactgcctt agtaaataaa ctcaaataat gttccatcta
     2101 cttgctcctt aatggtgagt ttgtgctttg ctatggggag caattttaaa ttcggtactt
     2161 tcttgttggt cagttttcca tgagcgtaat gcccaaatgg ttaagccaat taaaaagaaa
     2221 gctgaaggtg gcaacagtaa taggccatta ggaatatacc aaccaccatt attcactgtt
     2281 tgcatgatgg taaaaccaaa cactgttccc gaaccaaata actcacgaaa aaacgccaat
     2341 aacattaata acgctgaata acctaaacca ttaccaaagc catcgacaaa actaattaca
     2401 ggtggatttt tcatggcaaa ggcttctaaa cgccccataa caatacagtt ggtaataatt
     2461 aaacccacaa ataccgacat ctgcttggca atttctggca caaaggcttg cagtaattgg
     2521 tcaacaataa tcactaatgt cgctacaacc accatttgca caataatacg aatcgaactg
     2581 ggaatcacgt tacgaatcat actgataaaa aagttggaaa atgcagtcac taacgtcaaa
     2641 ccaattgaca tcgctaaagc atttttcatg gtgctagtca ccgctaatgc cgaacaaata
     2701 cccaacacct gaaaaatcac agggttagta tttaaaatcg ggcctaataa tatttctttg
     2761 gtactttgtt gttcggccat gttaaatacc cccttcgcgt actttgctca agaatttttt
     2821 atagcccaaa tcacccatcc aaaattggac aagattggtc acaccattac tggttaaggt
     2881 tgcacctgat aaaccatcga cttgatgaat agcatcaggt ttggctgggt caacattacc
     2941 tttaataatt ttgaaatggg tttgaccatc agcactgtaa acttgtttat taatccacaa
     3001 agctttccat ttgggattat cgacctcacc acccaatccc ggtgtttcgg catgttcaac
     3061 aaaggtcact cccataatag tattagcatc attttttaac actaaataac catataaagt
     3121 tgaccataaa ccataaccag aaacaggtag caccattttt tcgattttac ctgcggcatc
     3181 acgcaccaca taaatatcgg cttctttagc acggcggcgt aagcctgcca tatcttgatc
     3241 agccgttaat gccatcgaca aactgctatt tttgagtgct ttacgttggt cgtaagggtc
     3301 atcaagtttg acttctctaa attcgcctgt atctaaatta accaaataac gctgaaattt
     3361 ggcaaactct tgattgacgc gggctttgtc tccctcacct tcgggcagaa cttgtgctgc
     3421 ggccaaaatt ttacgttgtt tttcaagtgc cttattttca tcttgaatcg gctttaataa
     3481 agtcactgct gctgctaaag caatcgaaca caccaccgac aagacactgg caacaacgag
     3541 tgtttttcct gtagattctt tagacattgc gtaatgccct ccgtttgatg ttggcttgca
     3601 taacaaagta atcaatcagt ggcgcacata aattagcaaa taaaatcgcc aacatcatgc
     3661 cttctggata ggcaggatta accactcgaa ctaagaccac cattacacca atgacaatac
     3721 caaacactaa acggccagta ttggtcattg cggctgaaac ggggtcggtt gccataaaca
     3781 ccataccaaa ggcaaaacca cccaacacca aatgccagtg ccacggcatg gcaaacattg
     3841 ggttagtatc agagccaatt tggttaaaga taaaggacat ggttgccatg ccaatcatga
     3901 cacctgccat aatccgccat gatgctacac gggcaattaa taaaatcgcc gcaccaatca
     3961 aaatggctaa agtagaggtt tcacccacag agccagcttc aaaaccgaca aaggcatccc
     4021 accatgataa aggttgtaag ccagttgcgg tcatgtgttg tacacctgta taagcggcta
     4081 atttagcttg acctaaagca gttgcacccg aaaaaccatc aacacctaat ttccagactt
     4141 cgggactaat tgctgtccaa acggtatcac cagacatata ggcaggatag gcaaagaata
     4201 agaacgcacg ccccgctaag gctgggttaa taaagttttt gcctgtacca ccaaacactt
     4261 ctttaccaat cactacacca aagctaatgc ctaaagccac ttgccataaa ggcacagtgg
     4321 gcggacaaac taaggcgaat aacacagagg taacaaaaaa accttcgtta acttcatgac
     4381 cacgtttaat ggcaaataaa acttcccaaa aaccacccac aataaaggtc acggcataaa
     4441 ttggcacaaa ccacgttgcc ccatacacca aacaatccca ccaagaattg gcatcaaaac
     4501 ctgccaataa ggcaatcagc caatgccgcc aaccgtcaat cgccaactct ggggtcacgg
     4561 ctggattatg ttgcatagca taagccaacg tacctaaagc ttggttaccc acattccaca
     4621 taccaaaaaa catggcaggg aaagtagcca tccacactaa aatcataatt cgctttaagt
     4681 ccaccgaatc acgaacatga gagcctgcat gagtgacatt aggaggacta tataaaaaag
     4741 tatccactgc ttcatacaag ggataccatt tttcatagcg accacctttt tcaaaatggg
     4801 gtgccatgtt atctaatttg ttccgcatcc atttcataat tgtttagccc tctttctcaa
     4861 tacggtctag gacatctcgg aggatcggcc catattcata tttacctgga caaacaaagg
     4921 tacataatgc caaatcttct tcgtccagct ctaaacagcc taaagactgt gccatgtctg
     4981 tatcacctac caataagtag cgtaatagct gcgttggcaa aatatctaaa ggcataatat
     5041 cttcataaat accaacaggt accatggcgc gtgggctacc attactactc gtggtcatgt
     5101 tgaatttttt gcccaaccag cgaccaagat aaacacccaa tgcagaaaaa cgatgactac
     5161 ctaatttcat gtagtgcaac atttcacgct ctttggcttc tggaatcacc gatacttgca
     5221 aatgataacg gcctaaatat gcttctaccc cttgcgcatg acgtcctgac aacaccgaac
     5281 ccgaaataat ccgattatcg ctgccagaca actcacctgt ggtcatgtct aataacgatg
     5341 caccaacaat agttttggct aaacgtggtt gtttaacgct ggcacctgcc aaggcaataa
     5401 ttcgggtgct atctaactcg cctgtggcga ataatttacc aatcgcaatc acatcttgat
     5461 aattgagatg ccacaccaca ctgcttgcag aggctggatg taagaaatga atatgcgtgc
     5521 cgactaaacc tgcaggatgc gcgccactaa actcatgggc attaaatttt cctgtagaga
     5581 attgtgcttt ttcggcttta cagacataca atttgtcggc caacttagac actaaggcta
     5641 agccatcttc aaaggcttct tgttgttgtt ttatgacaac ttctgggtca gccgctaaag
     5701 gattggtatc cattgcattg acaaaaattg cactgggcgt tgttgtgggg tcgggtattt
     5761 tactaaatgg acgagtacga atcgctgtcc acaaacccga tgccaacaat tgctcaatca
     5821 ctccatcacg gttcagattg gctaattgat cccgattata ttgcctaaaa gtaatttgct
     5881 cgctgctatt actttcacaa tcaatcacta aagactgaaa tacacgcttt gcaccacgat
     5941 taattgctct aacccgacct gtcacaggtg cggtaaaaat cacttgggga ttttttttat
     6001 cggcaaataa tggctgtcct ttcaccacac gatcaccttc ggccactttc atggtgggct
     6061 tcatacccat ataatctgca cctaaaatag ccacagatcg tactgaagac atatcgctga
     6121 tttgttgtga cggtgcgcca gtaattggaa tatccaaacc tcgttttatt ttgaccatca
     6181 tgcactactc aacaactaaa aatctatttt tagaaaattt taagcaaaat tgacactttt
     6241 aggacttacg cggttatcgc ttatttgttc acatttcaat cgttttcaac gatttcatgt
     6301 tcacaaaacg cgctaatttt agtgaaatgc gtaagtcctg attttattta agctacttta
     6361 gacctgaatg atacaccgta actcacaaga gcatcacttc gcaacaatac gagatactct
     6421 ctatccatca ttgttatatt tttgatagaa aaatattata gcgaatgatt gtataacaag
     6481 tttttgcgct agtcctacat tctgactttt ttttaacggc tttttctgat tttag
//
LOCUS       DAOQMB010000090         6507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5000_length_6507_cov_17.218382, whole genome shotgun sequence.
ACCESSION   DAOQMB010000090 DAOQMB010000000
VERSION     DAOQMB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5000_length_6507_cov_17.218382"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1763
                     /locus_tag="PLY05_04745"
     CDS             <1..1763
                     /locus_tag="PLY05_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004913935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC02644.1"
                     /translation="QDTTATGLSDIDERIEPVVIEEIAPTLAQAIPDAALISLEQDLM
                     DDVVFDDDEDFVEPAKPVVTPAQAINQALAAPPTGKYWQPTMDLPSLELLEKPDLNRK
                     AGFTPEQLERLSRLLEIKLKEFNVDAKVVDALPGPVITRFEVDLAAGVKVSKITNIAK
                     DLARSMAMVSVRVVEAILGKTVIGIEVPNENRQIVRFKEILDTPVYQEKESPITMALG
                     KDISGLPMVADLARMPHLLVAGTTGSGKSVGVNAMILSILYKAKPEEVRLILIDPKML
                     ELSIYDKIPHLLTPVVTDMKDAANALRWSVAEMERRYKLMAAVGVRNLAGFNTKLRDA
                     EARGENILDPLWKKEDSGLQDAPPRLEPMPFIVIVIDEFADMMMIVGKKVEELIARIA
                     QKARAAGIHLILATQRPSVDVITGLIKANVPSRIAFQVSSKIDSRTILDQGGAEQLLG
                     HGDMLFQPIGSNLPVRIHGAFVSDDEVHRVCDHWRSCGEPNYVDEILEDYNDDGTPAK
                     GATGGMGDEDADAEQDVLYDDAVAFVLETRRASISFVQRKFKIGYNRAARLIEAMEMA
                     GLVSSMQSNGTREVLVPNNN"
     gene            1850..2476
                     /gene="lolA"
                     /locus_tag="PLY05_04750"
     CDS             1850..2476
                     /gene="lolA"
                     /locus_tag="PLY05_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HNC02645.1"
                     /translation="MYRYALPFIFLIAHSNIVQADQAASQKLNVLLEKVTSMRANFEQ
                     HTIDPKGQTLQTLTGQMSVQRPYLFRWDTQKPFTQQVIANGQVVWIYDPDLQQATKQK
                     LDKQVGNTPALLLSGDSKKIAQSFTVSQEKGLSAKQSVFILKPKDKEAVFESLKLTFN
                     NQSLNSMQLKDSLGQRTDIFFSQMQVNGKIAPSFFQFTPPKDVDVIDE"
     gene            2495..3769
                     /gene="serS"
                     /locus_tag="PLY05_04755"
     CDS             2495..3769
                     /gene="serS"
                     /locus_tag="PLY05_04755"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004972265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNC02646.1"
                     /translation="MLDAKLLRSQPDVVAARLQCRQVELDTAKLQAFEEQRKSLQTET
                     ETLQAERNAGAKKIGIAKKNGEDVAPIMARMQEVNERLETVKVELENLQHDINAFSMA
                     IPNLPHESVPQGKGEEDNVEVRRWGNIPQFDFEPKDHADLGEALGLIDFATATKITGS
                     RFSVIKGGLARLQRALTQFMLDLHTAEHGYQEVYVPYLVNADSLKGTGQLPKFEEDLF
                     KLQGEKPFYLIPTAEVPVTNIARDEIIAAEQLPVKYVAHTPCFRSEAGSYGKDTRGLI
                     RQHQFEKVELVQLVRPEDSYQALEELTGHAEKVLQLLGLPYRVMLLCGGDMGFSAAKT
                     YDLEVWLPAQNKFREISSCSNFEAFQARRMQARYRDKDSKPELLHTLNGSGLAVGRTL
                     VAILENYQQADGSIRVPEVLQAYMGGVKVIGG"
     gene            3772..4248
                     /locus_tag="PLY05_04760"
     CDS             3772..4248
                     /locus_tag="PLY05_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02647.1"
                     /translation="MLVSTEVAQRFLAAYKELMQALNQGVEPDSMEKYAQLREQIYYY
                     RDNHPQVYENALVPDWLAVIKQAVFGDFIYLKKYQKGYVFQHVNTGAFYQVKALTTAL
                     EETLPEFMIVRTALLNFDGEWLCDGLVLHKTALSKDDARMARDTYAEVKKQKQVIG"
     gene            4248..5630
                     /gene="cysG"
                     /locus_tag="PLY05_04765"
     CDS             4248..5630
                     /gene="cysG"
                     /locus_tag="PLY05_04765"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HNC02648.1"
                     /translation="MDYLPVFLKIAEQPCLVIGGGHIAKRKVTLLLKAHAKVTVIAPD
                     ILPELKEQVLANGGKIIEQVYHAQHLAQQRLVIAATDDEALNQQVFNDCELANIPVNV
                     VDAPALCRFIFPSIIDRSPVVIAISSSGQSPVLARLLRTRLESMIPAAYGQLAAFVGK
                     FRQQVQQVLPDTSNRRAFWEKELQGRFAELVYNGRHDAAEQHLQQALATHQQHAGEVY
                     LVGSGPGDPDLLTFKALRLMQQADVVVYDRLVSQPILDLCRRDADMIYVGKARADHAV
                     PQEDINQLLVRLAQEGKRVCRLKGGDPFIFGRGGEEIEELAAAGIAFQVVPGITAASG
                     CSAYAGIPLTHRDYAQSVRFVTGHLKDGTPNLPWSELVHERQTLVLYMGLVGLEEIAK
                     QLIANGMRPDMPVALVSHGTRPNQQVVTGEIATIADKVHESGIKAPTLTIIGDVVKLQ
                     DKLAWFGVTK"
     gene            5703..5960
                     /gene="vapB"
                     /locus_tag="PLY05_04770"
     CDS             5703..5960
                     /gene="vapB"
                     /locus_tag="PLY05_04770"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC02649.1"
                     /translation="MPSISLETQKTTAKLFMNGRSQAVRLPAFCRFEGQEVMIEKVGD
                     KVILTPYRSNWLDYFASEQRPTDDFMQEQETMRFSERASFE"
     gene            5957..6358
                     /locus_tag="PLY05_04775"
     CDS             5957..6358
                     /locus_tag="PLY05_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC02650.1"
                     /translation="MSKILLDTNICIYVMNRRPIEVLKKFQQYSQHLAISSIVLAELR
                     FGASHSSKPEHNHQQVNQFCQCLDIVPFDEQAAFYYGEIRTKLKYQGQLIGDNDLLIA
                     AHALALNALLVTNNVKEFQRVDGLNVENWIL"
BASE COUNT         1959 a         1209 c         1423 g         1916 t
ORIGIN      
        1 tgcaagacac caccgctaca ggtttaagtg atattgatga gcgtattgag ccagtggtga
       61 tcgaagagat agctccgaca ttggctcaag ccatacctga cgcggcttta attagtcttg
      121 aacaagattt aatggatgac gttgtttttg acgatgacga ggattttgtt gagccagcaa
      181 aaccagtggt tactcctgct caagcaatta atcaggcttt agctgcacca ccaacaggta
      241 aatattggca acccaccatg gacttgccct cattagaact actcgaaaaa cccgatttaa
      301 atcgcaaagc aggttttacg ccagaacaat tagagcgttt atcacgtttg ttagaaatta
      361 aacttaaaga gtttaatgtt gatgccaaag tggttgatgc tttacctggc ccagtgatta
      421 cccgttttga agtggattta gccgcagggg taaaagtttc taaaattact aatattgcca
      481 aagatttagc acgttctatg gcaatggtct cggtgcgtgt ggttgaagcg attttgggca
      541 aaacggtgat tggtatcgaa gtgcctaatg aaaatcgtca aatagttaga tttaaagaga
      601 ttttagatac gcctgtttat caagaaaaag aatcacccat taccatggct ttaggcaaag
      661 acattagtgg tttgcccatg gttgccgact tagccagaat gccgcactta ttagttgcag
      721 gtacaacggg ttcaggtaaa tcagtgggcg taaatgccat gattttaagt attttataca
      781 aagccaaacc tgaagaagtg cgtttaatct taattgaccc aaaaatgttg gaattgtcta
      841 tttacgataa aatcccgcat ttattaacgc ctgttgttac tgacatgaaa gatgccgcca
      901 acgctttacg ttggtctgtc gcagaaatgg aacgtcgtta taaattaatg gcggctgttg
      961 gtgtgcgtaa cttagcaggc tttaatacca aattacgtga tgctgaagcc cgtggcgaaa
     1021 atattcttga tcctttatgg aaaaaagaag attcaggttt acaagacgcg ccaccacgtt
     1081 tagaacctat gccatttatc gttattgtga ttgacgaatt tgctgacatg atgatgattg
     1141 tgggtaaaaa agttgaagag ttaattgcac gtattgccca aaaagcccgt gccgcaggga
     1201 ttcacttaat tttggcaacc caacgtccat cggttgatgt gatcacaggt ttgattaaag
     1261 ccaacgtgcc atcgcgtatt gccttccaag tgtcgagtaa aattgactca cgtaccatct
     1321 tagatcaagg cggtgcagaa caattattag gtcatggtga tatgttgttc caaccgattg
     1381 gcagcaactt acctgtacgt attcatggtg cgtttgtcag tgatgatgaa gtgcatagag
     1441 tgtgtgatca ttggcgttct tgtggtgaac ccaattatgt cgatgaaatc ttagaagatt
     1501 ataacgacga tggcacaccc gccaaaggtg caacaggcgg catgggtgac gaagatgctg
     1561 atgccgaaca agacgtatta tatgatgatg cagtcgcttt tgtgttagaa acgcgtcgag
     1621 cctctatttc ctttgtacaa cgtaaattta agattggcta caaccgtgcc gcacgtttaa
     1681 ttgaagcgat ggaaatggcg ggcttagtta gctcaatgca aagtaatggc acacgtgaag
     1741 tgttggtgcc caataataat tgatacacaa gcgcgtcaca gcaacaacag tgctgcaacg
     1801 tgccttcagt taagattgag caacttctca gcaaaaatgg aaagaactta tgtatcgtta
     1861 tgctttgcct tttatctttt taattgcaca tagcaatatc gtccaagctg accaagccgc
     1921 tagtcaaaaa ctaaatgtgc tattagaaaa agtcaccagt atgcgcgcca actttgagca
     1981 gcataccatt gaccccaaag gtcaaaccct gcaaacctta acagggcaaa tgtctgtaca
     2041 acgtccttat ttatttcgtt gggataccca aaagccgttt acccaacaag tcattgccaa
     2101 tggtcaagtg gtttggattt atgatccaga cctacaacaa gccaccaaac aaaaactgga
     2161 taagcaagtc ggcaacacgc cagccttatt attatcgggt gatagcaaaa aaattgcgca
     2221 atcattcacc gttagtcaag aaaagggctt gtcagccaaa caaagtgttt ttatcctcaa
     2281 gcccaaagat aaagaagctg tttttgagag tttaaagctg acttttaaca atcaaagcct
     2341 caattcgatg caattaaaag attctttagg gcaaagaacc gatattttct ttagtcaaat
     2401 gcaagttaat ggcaaaattg cgccatcttt ttttcaattc acaccaccaa aagatgttga
     2461 tgtgattgat gagtgatttc taggattatt tttcatgtta gacgctaaat tattacgtag
     2521 ccaaccagac gttgttgctg ctcgcttaca atgtcgccaa gttgaattag atactgccaa
     2581 attacaagct tttgaagagc aacgtaagag cttacaaacc gaaaccgaaa ccttgcaagc
     2641 tgagcgtaat gcaggggcaa aaaaaattgg tattgccaaa aagaatggcg aagatgttgc
     2701 cccgattatg gcacgtatgc aagaagtcaa cgaacgttta gaaacggtta aagttgaact
     2761 tgaaaatcta caacatgaca ttaatgcttt tagcatggct attccaaact tgccacatga
     2821 gtctgtacca caaggcaaag gcgaagaaga caatgttgaa gtacgtcgct ggggtaatat
     2881 tcctcaattt gattttgagc caaaagacca tgccgatttg ggcgaagcct taggtttaat
     2941 tgattttgcc accgccacca aaattacagg atctcgcttc tcggtgatta agggtggttt
     3001 agcgcgttta caacgtgctt taacccaatt tatgttggat ttacatacgg ctgagcatgg
     3061 ttatcaagaa gtgtatgtgc cttatttggt aaatgctgat agtttaaaag gcacaggcca
     3121 attgcctaaa tttgaagaag acctgtttaa attacaaggc gaaaaaccct tttatctaat
     3181 tcctactgcc gaagtgccag tgactaatat tgcgcgtgac gaaattattg ctgccgaaca
     3241 attgcctgtt aagtacgtgg cacatacacc ctgttttaga agtgaagcag ggtcttatgg
     3301 caaagatacc cgtggtttga ttcgtcaaca tcaatttgaa aaagttgaat tagtgcaatt
     3361 ggtacgtcca gaagattctt accaagcttt agaagaatta acaggtcatg ccgaaaaagt
     3421 attgcaatta ttaggcttac cataccgcgt gatgttgtta tgtggtggtg atatgggctt
     3481 ttctgcggca aaaacctatg acttagaagt gtggttacct gcccaaaaca aattccgcga
     3541 aatttcttcg tgttcaaact ttgaagcctt tcaagcacgt cgtatgcaag cacgttaccg
     3601 tgacaaagac agcaagcctg agttgttaca taccttaaat ggttctggtt tggcagttgg
     3661 ccgtacctta gtggcgattt tagaaaatta ccaacaagca gatggttcga ttcgtgtgcc
     3721 tgaggtttta caagcctaca tgggcggtgt taaggtgatt ggaggctaat tatgttggta
     3781 tctacggaag ttgcacaacg ctttttggca gcctataaag aattaatgca agccttgaat
     3841 cagggagttg aacctgattc aatggaaaaa tatgcacaat tgcgtgaaca gatctattat
     3901 taccgtgata atcatccaca agtttatgaa aatgcacttg tacctgattg gttagctgtc
     3961 attaaacagg ccgtatttgg cgattttatt tatttgaaaa aataccaaaa aggttatgtg
     4021 tttcaacatg tgaatactgg tgctttttat caagtaaaag cattaacaac cgctttagaa
     4081 gaaaccttac ctgagtttat gattgttcgt acggccttat taaattttga tggcgaatgg
     4141 ttgtgtgatg gtttggtatt acacaaaaca gccttaagca aagacgatgc gcgtatggct
     4201 cgtgatacct atgctgaagt gaaaaaacaa aagcaggtga ttggctgatg gattatttac
     4261 cggtctttct aaaaattgct gagcaacctt gtttggtgat tggtggtggg catattgcca
     4321 agcgtaaagt aacgttactg ctcaaggcac atgcaaaagt cacggtcatt gctccagata
     4381 ttttacctga gctaaaagag caagtgttgg ccaatggtgg aaaaatcatc gaacaagttt
     4441 accatgctca acatttagct caacagcgtt tggtcattgc agcaacggat gatgaggctc
     4501 tcaatcaaca agtctttaac gattgtgagc tggctaatat tcctgtcaat gtcgttgatg
     4561 cacccgcttt atgccgtttt atttttccgt caattattga tcgctctccc gtggtaattg
     4621 ctatttcgag tagtggtcag tcgccagtcc tagcaagatt attaagaacc agattggaaa
     4681 gtatgattcc tgcggcttat ggtcaactag ctgcttttgt gggtaaattt cgtcaacaag
     4741 tgcaacaagt attgccagac acctcaaaca gacgtgcttt ttgggaaaaa gaattgcaag
     4801 gacgttttgc tgagttagtg tataacggcc gtcatgatgc tgccgaacag catttacaac
     4861 aagctttagc cacacatcaa caacatgcag gcgaagtata tttagtcggt tctgggccag
     4921 gtgatcctga tttattaacc tttaaagcct tgcgcttaat gcaacaagcc gatgttgttg
     4981 tgtatgatcg tttagtgtct caacctattt tagatctttg ccgccgtgat gccgacatga
     5041 tttatgtggg taaagcgcgt gccgatcatg ccgttcccca agaagacatt aatcaattgt
     5101 tagtaagatt agcgcaagag ggtaaacgtg tttgtcgcct aaaaggtggt gatccgttta
     5161 tttttggtcg tggtggggaa gagattgaag agttagcagc agcaggcatt gcttttcagg
     5221 ttgtacctgg aattacggcg gccagtggtt gttctgctta tgcaggcatt cctttaactc
     5281 atcgtgatta tgcccaatca gtgcgttttg tcacaggtca tttaaaagat ggcacaccaa
     5341 acctgccgtg gtctgaatta gttcatgaac gtcaaaccct tgttttatat atgggcttgg
     5401 tgggtttaga agagattgct aaacaactca tcgctaatgg tatgcgtcct gatatgcctg
     5461 ttgccttagt ctctcatggc acacgcccca atcaacaagt agtcactggt gaaatagcta
     5521 ctattgctga taaagttcac gaaagtggta tcaaagcacc aacgttgacg attattggtg
     5581 atgtggtcaa gttgcaagat aaattggcgt ggtttggcgt aacaaaataa atagaattgt
     5641 gttgttgtta tttaggtcga gatatatact tggtatatat ctcgatttgg gagtaactca
     5701 ttatgccatc tatatctttg gaaactcaaa aaacaacagc aaaacttttt atgaatggcc
     5761 gtagtcaggc tgtgcgttta cctgcatttt gtcgttttga agggcaagaa gtgatgatcg
     5821 aaaaagtagg tgataaagtc attctgaccc cttaccgttc aaattggcta gattattttg
     5881 ccagtgaaca acgccctaca gatgatttta tgcaagagca agaaacaatg cgcttttcag
     5941 agcgagctag ttttgaatga gtaaaatttt attagatact aacatctgca tttacgtaat
     6001 gaaccgcaga cccattgagg tattaaagaa atttcagcaa tatagccaac atttagctat
     6061 atcaagtatt gtcttagcag agttgcgctt tggagcaagc cactctagca aaccagaaca
     6121 taatcaccaa caagtaaatc agttttgtca gtgtttagat attgtaccat ttgatgaaca
     6181 agcggcattt tattatggtg aaattcgtac aaaattaaaa tatcaaggcc aattaattgg
     6241 cgataatgat ttgttaattg cagctcatgc tctagcatta aatgcgctgc ttgtgaccaa
     6301 taatgttaaa gaatttcaac gtgttgatgg actgaatgtg gaaaattgga ttttataaca
     6361 tataaaaata agtgataatg cagttttcta tttataaaac tgcataaaaa acgtatatcc
     6421 atcagttttg ctaataatta atatgattta attgcatgaa gatatagcgg aattgtctca
     6481 ataacttatc tgatttttgt taaaata
//
LOCUS       DAOQMB010000091         6475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5031_length_6475_cov_15.626324, whole genome shotgun sequence.
ACCESSION   DAOQMB010000091 DAOQMB010000000
VERSION     DAOQMB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6475
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5031_length_6475_cov_15.626324"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1569
                     /locus_tag="PLY05_04780"
     CDS             <1..1569
                     /locus_tag="PLY05_04780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC02651.1"
                     /translation="KRQKAIYGQGILEGNTYFVKIAAGHSEMVAGNRADSGLVTFLNE
                     PDLKSKQDYLNISLENNTFSQHQIKFQTDFSQFESKQQIRVKTPAGYFIPELQQLFVQ
                     DRVYTDNFLNAVSKILAGKRASLPTVTSPQIGALTLQVACKTGLQQGKACGSIKPDPV
                     YTKELIYLSDINKKESRLEAEIQDTWTINPNLRIVYGVGIQDSRADSLHYFNGEVENT
                     VWRVFAHGEWQFMPDWRLNAGIMDEHDDYSGSLKSPRLALNWRFLPTHSLRLVSSRAY
                     RTPDLRESKAYWQYTAYTEDRSLSKYDGNFPFLGQAPSPVGQTCVRTNTNTGVMDICT
                     DGAPSERITSAEIGYYGELPKQHLQLDIRVFKDNLKLSEHNLEIDDFVIAPLVEHQQR
                     GVELSTQWRPHANWRFMLNYAYDDITGGNDNTDFVPKHSGNFGAWYDTGKGIQSSVNY
                     VFYNQLYNDPDDGGLYFDRLDWRVAKKWSMRKLHDVELSGVWQIRLTEDRELRRSNGS
                     PRDKMWLQLSYAFD"
     gene            1762..2634
                     /locus_tag="PLY05_04785"
     CDS             1762..2634
                     /locus_tag="PLY05_04785"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNC02652.1"
                     /translation="MLKKLCFYAVLLLCWLSHSAYAQTQVQILTTNADESSGMLAYLT
                     KQLKDEANVFIADTPKVQAELLIVFAYDKAQQLPTSRVPALFVLAQPSDISIEKNDGV
                     LYWTPSLAAQLALIKEIQPAVNKIGMLANPQNEDLSWLRSFKQYALEQGVEVRVQTVD
                     KSRIGRQVSDLAVSTDILLAQPDTYIYNRETIRFILLAAYRQNKVLIGPSPAFVNAGG
                     LASLYASSESIYEELAQQIKQFISTRKLTPATRVKKMTVSFNPQVAKSLGLIIPDIKQ
                     VEQKIQKKELPSWP"
     gene            2625..4511
                     /locus_tag="PLY05_04790"
     CDS             2625..4511
                     /locus_tag="PLY05_04790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC02653.1"
                     /translation="MAIKTPWRNLRIRILVLAIVPAILMLICLLTYHVLARLHNAKQE
                     QENFGQIIAMQLASSADFAIITENADSLVPQVNTILAQPMVSSVSVMKADKTVILSKQ
                     NKQALEEAQLHYSVPIYQQSLQLDDNDWLVEKAPKQDKQTILGFVDVGLNAEYIYDKE
                     RKFVFKSALLAIAVLLGVVGLAVWMAIGLEKPLRAIIGLVGALKNRQFDARVHIKQNG
                     ELGALAYHLNLLAAMLEDNRNSQVKNTEELMTARQRADKANKAKSEFLAMMSHELRTP
                     LNGIFGGLQLLDTQSLSVNDQQNVQDVLKASSQLKQLIDDILDFSKIEEQRLILSTQA
                     FLPKVAMQQLVERIAIDVKNKGLEFVFEFEGRDDLWLKGDEMRIRQLLIKLIDNAIKF
                     TEKGRVGLRVKVSPYNQSQAQLLCEVFDTGIGISQQMQAHIFQPFTQADRSHTRSFGG
                     AGLGLAIAWSLSRLMQGDLRVESELLVGSCFCFEVTLAICEVCAKESQPVVVNDKKRV
                     FNAHVLVVDDNPANRKVAEAMLRVAGCTVTTANNGQEALVHLSAGSIDLVLMDCQMPI
                     MDGYEATRLWREQEMEKRIPIIALTANASTENETVCINAGMDVMLSKPFRKQQLEMLL
                     SAWL"
     gene            complement(4506..5171)
                     /locus_tag="PLY05_04795"
     CDS             complement(4506..5171)
                     /locus_tag="PLY05_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HNC02654.1"
                     /translation="MKHHIFLNQLTWSTQQTHNHLLKLNKDEQQQAKIYTHPLRLRAF
                     VASRLMLKQALNLLNNTQQDWLFAKDQGRLIIRYPAVKWYISLTHSDDYLACLLSPQP
                     YCGVDLEQRMYKPRFLAIAKRFFATDEFLHLNTLAEDQAFICFLDWWTRKEACIKAWH
                     VGIAHHLNSIVFNPSQLCPIRYPQQFKHIPLQLHTISSPHLQIACAVNDTNPEWFVHH
                     LTL"
     gene            complement(5168..5995)
                     /locus_tag="PLY05_04800"
     CDS             complement(5168..5995)
                     /locus_tag="PLY05_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNC02655.1"
                     /translation="MFHRTLLFIGVNLLANASFALPKEQLTAGGIAIIPLPSYISENA
                     QVKFGSNLVPVITREDALFAVVGIPLSHKEPELSITITDNQLSDHLTVQLSNKDYPEQ
                     RITLQETQSNKVTPSEDELARYSREATEQKTVYQLFSSSQTKSWPNFAWPIKGRISSP
                     FGLKRFFNDQPRDPHSGLDVAAPEGKAIAAPANGIVAQTGDYFFNGKTVMIDHGQGII
                     SMLCHLSKIDVAKGQLIKQGDIIGLVGQTGRATGPHLHYGVSINNARIDPLLVLPTP"
     gene            6062..6436
                     /locus_tag="PLY05_04805"
     CDS             6062..6436
                     /locus_tag="PLY05_04805"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC02656.1"
                     /translation="MAVGDKIVVGMLVIAGLAWWQQDNLKKQWQKMQTTPAPIATKTE
                     VYTWKDKEGTVHYSTTPEHKNATQTIVDTGKISRLEPLPQKQEAPKEEKLLLHEVRED
                     MIQNRNKMQETREKQMMQEIER"
BASE COUNT         2103 a         1082 c         1354 g         1936 t
ORIGIN      
        1 aaacgccaaa aagccattta tggtcaaggg attttagagg gaaatactta cttcgtaaaa
       61 atagctgctg gtcattcaga gatggtggct ggtaatcgtg ctgatagtgg tttggtcacg
      121 tttttaaatg agccagattt aaaaagcaaa caagactatt taaacatttc tttagaaaac
      181 aatacctttt ctcaacatca aattaaattt caaacagatt tttcgcaatt tgagtctaaa
      241 caacaaatta gagttaaaac gccagcaggc tactttattc ctgaattaca acaattattt
      301 gtgcaagata gagtttatac agataacttt ttaaatgcag tcagtaaaat tctagcaggt
      361 aaaagagcat ctttacccac agtgactagt ccacaaattg gtgctttaac attgcaagtt
      421 gcttgtaaaa cgggtttgca acaaggcaag gcctgtggct caattaagcc tgatcccgta
      481 tatacaaaag agctgattta cttatcagat attaataaaa aagaatcacg tttagaagca
      541 gaaatacaag atacgtggac aattaatccg aatttacgca ttgtgtatgg tgtaggtatt
      601 caagactctc gagctgactc tctacattat tttaatggtg aagtcgaaaa tactgtatgg
      661 cgtgtttttg cacatggtga atggcaattt atgcctgatt ggcgtttaaa tgcaggtatt
      721 atggatgagc atgatgatta ttcggggagt ttaaaatcac ctcgtttagc cctgaactgg
      781 cgttttttgc caacacattc tttgcgttta gtatcttctc gagcttatcg cacaccagat
      841 ttacgtgaat caaaagcgta ttggcaatat actgcatata ctgaagacag aagtttgagc
      901 aaatatgatg gtaatttccc ctttttagga caagctcctt cacctgtggg gcagacttgt
      961 gtgcgaacca atacaaatac aggcgtcatg gatatttgta ctgacggcgc acccagtgag
     1021 cgtattactt ctgctgaaat tggttattat ggtgaactgc caaaacaaca tttacagtta
     1081 gatattcgtg tatttaaaga taatttaaaa ttatccgagc ataatttaga aattgatgat
     1141 tttgtgatag cccccttagt tgagcatcaa caacgaggtg tagagttatc aacgcaatgg
     1201 cgtcctcatg ctaattggcg ttttatgctc aattatgctt atgatgatat tacgggcggc
     1261 aacgataata cggactttgt gccaaagcat agtggcaatt ttggtgcatg gtatgacacg
     1321 ggtaaaggca tacagtcaag tgtaaattat gtattttata atcaacttta taatgaccct
     1381 gatgatggtg gtttatactt tgatcgttta gattggcgtg tcgctaaaaa atggtcgatg
     1441 cgtaagttgc atgatgtaga gttgtcgggt gtttggcaaa tacgcttgac cgaagataga
     1501 gagttacgcc gtagcaatgg ctcaccacgt gataaaatgt ggttgcagct aagttatgca
     1561 tttgattgat aaatcgggac ttatgcattt tatcgcaagc atgtattgag cgaagttgaa
     1621 acaagcgcgt ttgctcctct cagtccttct ttaataagga aggaagccaa aggcagaggg
     1681 tgaatgtgag tcaataagcg ataactgcgt aagtcctata aatgttgttt tagatgagta
     1741 gcacaaggga tggtgcgtat tatgttaaaa aaactgtgct tttatgctgt attactcttg
     1801 tgttggttaa gtcatagtgc ttatgcccag acacaagtac aaatattaac gaccaatgcc
     1861 gatgagtcgt cggggatgct tgcttatttg actaagcagc ttaaagacga ggctaacgtt
     1921 tttattgcag atacgccaaa agtacaagcc gaattattaa ttgtttttgc ttacgataaa
     1981 gcgcaacaat taccaacaag tcgagtccca gctttatttg ttttagcaca accttctgat
     2041 atttctatcg aaaaaaatga tggtgtttta tattggacac ctagtttagc agcacaactc
     2101 gccttaatta aagaaataca acccgccgtt aacaaaatag gaatgttggc taacccccaa
     2161 aatgaagatt tatcatggtt aagaagtttt aaacaatatg ccttagaaca aggcgttgaa
     2221 gtcagagtgc aaacggttga taaaagccgt atcggacggc aagtatctga tttggccgtc
     2281 agtacagata tattattagc gcagccagat acctatattt ataaccgcga aacgattcga
     2341 tttattttat tagcagccta tcgtcaaaac aaagtgttaa ttggcccaag tccagcattt
     2401 gtcaatgcag gaggtcttgc gtctttatat gcctcctcag aaagtattta tgaagaacta
     2461 gcacaacaaa tcaaacaatt tattagcacg cgtaaattaa cacccgcgac acgtgtcaaa
     2521 aaaatgacag tgagttttaa tccgcaagta gcgaagtctt tggggttaat tattcccgac
     2581 attaaacaag ttgaacaaaa aattcagaaa aaggagttgc catcatggcc ataaaaactc
     2641 cttggcgtaa tttacggatc agaattttag tgttggcgat tgtgccagct atcctaatgt
     2701 taatttgttt gttgacttat catgtcttag caagattgca taatgctaaa caagaacaag
     2761 aaaactttgg ccaaattatt gccatgcagt tagcgtccag tgctgatttt gccattatta
     2821 ctgaaaatgc agatagcctt gttcctcaag ttaatacaat tttggcacag cccatggtgt
     2881 cttctgtttc cgtgatgaag gcagataaaa cagttatttt atccaaacaa aacaaacaag
     2941 cgttggaaga agcacaattg cattattctg tgcctattta tcagcaatct ttacagttag
     3001 atgataatga ttggttggtt gaaaaggcac caaagcaaga taaacaaact atccttggtt
     3061 ttgttgatgt gggtctaaat gcagagtata tctatgataa agaaagaaaa tttgttttta
     3121 aaagtgcatt attagccatc gcggtcttac taggtgttgt tgggttggct gtatggatgg
     3181 cgattggttt agaaaagccc ttaagagcca ttattggttt ggtcggtgca ttaaaaaatc
     3241 gtcaatttga tgcaagagta catattaaac aaaatggcga attaggagca ttggcatacc
     3301 atctcaactt attagcagca atgttagagg ataatcgtaa ttcacaagtc aaaaacacag
     3361 aggaattaat gactgctcgt cagcgagcag ataaagctaa taaagccaaa agtgaatttt
     3421 tagcaatgat gagtcatgaa ttaaggacac cactaaatgg tatttttggt ggactacaat
     3481 tattagacac ccaaagctta tctgtgaatg atcaacaaaa tgtacaggat gtattaaaag
     3541 catcttctca attaaaacaa ttaattgatg atattttaga cttctcaaaa attgaagaac
     3601 aacgtttgat tttaagtaca caagcatttt tacccaaagt agctatgcag cagttagttg
     3661 aacgaattgc tattgatgta aaaaataaag gtttagagtt tgtttttgag tttgaaggac
     3721 gtgatgactt atggttaaaa ggtgatgaaa tgagaattcg tcaactatta atcaaattaa
     3781 ttgataacgc gattaaattt accgaaaaag gacgtgtagg gcttagagtt aaagtaagcc
     3841 catacaatca gtcacaagcg caattattat gtgaagtatt tgatacaggt attggtatta
     3901 gtcaacaaat gcaggcacat atttttcaac catttactca agcagatcgt tcgcatactc
     3961 gctcttttgg tggtgctggc ttaggattag ccattgcttg gagtttgtct cgtttaatgc
     4021 agggcgattt aagagttgaa agtgagttat tggtaggaag ctgtttttgt tttgaggtga
     4081 ctttagcgat ttgtgaagta tgtgccaaag aatctcagcc agttgttgtg aatgataaaa
     4141 agcgtgtatt taatgctcat gtattagtcg tagatgataa tcctgctaat cgtaaagtgg
     4201 ccgaagcgat gctcagagtg gcaggatgta ccgtgacaac ggctaataat ggtcaagaag
     4261 ccttagtgca tttaagtgca ggcagcatag acttggtgtt aatggattgt caaatgccga
     4321 tcatggatgg ttatgaggcc actcgtttat ggcgtgagca agaaatggaa aaaagaattc
     4381 caattattgc cttaaccgct aatgcgagta cagaaaatga aactgtttgt attaatgctg
     4441 gtatggatgt gatgttaagc aaacctttcc gtaaacagca actcgaaatg ttattaagtg
     4501 catggttata acgttaagtg atgtacaaac cactctggat ttgtatcatt gactgcacaa
     4561 gcaatttgca aatgagggct tgaaatagta tgtaactgta gtggaatatg cttaaattgt
     4621 tgtgggtaac gaatagggca taattgcgat ggattaaaga caatgctatt taaatgatga
     4681 gcaatcccca catgccaagc cttaatacaa gcctcttttc gcgtccacca atccaaaaaa
     4741 caaatgaatg cttgatcttc tgctaatgtg tttagatgta aaaattcatc agttgcaaaa
     4801 aaacgtttgg caatcgccaa aaaacgtggt ttatacattc tctgttctaa gtcaacacca
     4861 caataaggtt gtgggcttaa taaacaagct aaatagtcat cactgtgcgt taggctgata
     4921 taccatttaa ccgcaggata ccgaataatt aaacgtcctt gatctttagc aaataaccaa
     4981 tcttgctgag tattattaag caaatttaat gcttgtttta gcatgagacg gctagcaaca
     5041 aatgctctta aacgtaaagg gtgggtatag atttttgcct gttgttgttc atctttatta
     5101 agtttaagca agtgattatg ggtttgttgt gttgaccatg ttaattgatt taaaaaaata
     5161 tggtgtttca tggggttggc aatactaaca gtggatcaat acgtgcattg ttaatactta
     5221 caccatagtg cagatgaggg cctgttgctc gacctgtttg ccccaccaaa ccaataatat
     5281 ctccttgttt tatgagttga ccttttgcaa catcaatttt gcttaaatgg cataacattg
     5341 aaataatgcc ttgaccatga tcaatcatca ctgttttgcc attaaaaaaa taatcacctg
     5401 tttgggcaac aataccattc gcaggtgcag caatggcttt tccttcggga gcagccacat
     5461 ctaaacccga atgagggtca cgcggttggt cgttaaaaaa gcgttttaaa ccaaacggac
     5521 tgctaattcg gcctttgatt ggccatgcaa aatttggcca tgattttgtt tgagaagacg
     5581 aaaagagttg ataaaccgtt ttttgctctg tcgcttcacg gctataacga gctaactcat
     5641 cttcacttgg tgtgactttg ttgctttgtg tttcttgcaa tgtgatgcgt tgttcgggat
     5701 agtctttgtt acttaattga accgttaaat gatcactaag ttgattatca gtgatcgtaa
     5761 tagatagctc aggctctttg tggcttaacg gaataccaac gacagcaaat aatgcgtctt
     5821 ctcgtgtaat aacaggcact aagttagagc caaattttac ttgagcattt tcagagatat
     5881 aactcggcaa tggaataatg gcaatgccgc cagcagtcag ttgttctttg ggtaacgcaa
     5941 agctagcgtt agctaaaaga ttaacaccaa taaataacaa tgtgcgatga aacatgataa
     6001 tttgtaaaat ataatgaaaa actacgattg aggatactct atttttatcg aggagcgaaa
     6061 tatggctgtg ggagataaaa ttgttgttgg catgttagtc atagctggcc tagcatggtg
     6121 gcaacaagat aatttgaaaa aacaatggca aaagatgcag accacaccag caccgattgc
     6181 cacaaaaaca gaagtgtaca catggaaaga caaagaggga acagtgcatt actccacaac
     6241 acccgaacat aaaaatgcaa cacaaaccat tgttgataca ggcaaaatta gtcgtttaga
     6301 gccgttgcca caaaaacaag aagccccaaa agaagaaaaa ttattgttac atgaggtgag
     6361 ggaagacatg attcaaaacc gtaacaaaat gcaagaaaca cgcgaaaaac aaatgatgca
     6421 agaaattgaa cgatagttaa cagattagcg acaatgttta attgaacaat tgggg
//
LOCUS       DAOQMB010000092         6442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5073_length_6442_cov_16.832003, whole genome shotgun sequence.
ACCESSION   DAOQMB010000092 DAOQMB010000000
VERSION     DAOQMB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6442
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5073_length_6442_cov_16.832003"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="PLY05_04810"
     CDS             complement(<1..547)
                     /locus_tag="PLY05_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNC02657.1"
                     /translation="MKKIILALSLSGALLGATPLYAEESAAPAAAPVATATVEASATP
                     APSPEAAPVAAPAEAAPAAAAPAPTANKGDDAFLMICSAFVILMSIPGLALFYGGLVR
                     KKNMLSVLMQVFTVFSLISVLWMIYGYSIAFTEGNAFFGSLSKVFLSGVTPDSLAATW
                     SKATPIHEMIYIVFQGAFAAIT"
     gene            complement(639..977)
                     /gene="glnK"
                     /locus_tag="PLY05_04815"
     CDS             complement(639..977)
                     /gene="glnK"
                     /locus_tag="PLY05_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_253975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNC02658.1"
                     /translation="MKLVTAIIKPFKLDDVRESLSDIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVAIGDDLVDRVIEAITKAANTGKIGDGKIFVTSLEQIIRI
                     RTGETGVDAV"
     gene            complement(1028..>1485)
                     /locus_tag="PLY05_04820"
     CDS             complement(1028..>1485)
                     /locus_tag="PLY05_04820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HNC02659.1"
                     /translation="GKAGEVAYDVGVMRYNYPGLNDNNAAFEADYNEVYASVSTMGAK
                     VGFNYSPDYFFESDKFLYLYASYGTEVAGVGLFGSVGMNKFDSAAMMSQALGTTGSDD
                     SYIDYKLAASKAISGITVEGAYIGSNIDEKECGAGLCEGRFVLTLTKGF"
     assembly_gap    1486..1585
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1855..2157
                     /locus_tag="PLY05_04825"
     CDS             1855..2157
                     /locus_tag="PLY05_04825"
                     /inference="COORDINATES: protein motif:HMM:NF016280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HNC02660.1"
                     /translation="MRYFGAFFILVRFLGEVDMVLDNLVQKVREQVRDRFEQANQTGA
                     WQELDKNLQSVLQEVFAKMELVTREEFDRQAQLLGEARMKLQMLEKQLKSLENQIK"
     gene            2207..3697
                     /locus_tag="PLY05_04830"
     CDS             2207..3697
                     /locus_tag="PLY05_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HNC02661.1"
                     /translation="MSLALVYSRAALGLHAPLVTVEVHISNGLPALTIVGLPEAAVRE
                     SKDRVRSALINAGFEFPQRRVTINLAPADLPKEGGRFDLPIALGILAASGQIITESLQ
                     HKEFVGELALTGELRPIIGVLSASLAATKEGHSLYVPVDNAQEAAYPQQAQIYGIRHL
                     KELCASLAGDVVLTATKNQTQHHHVKSPDLSDVKGQHRARRALEIAAAGGHSLLMMGP
                     PGTGKTLLASRLSGILPPLDDNQSIEVATIHSVAAQNYPHFGLPPFRAPHHTASAPAL
                     VGGGSNPKPGEISLAHHGVLFLDELPEFDRKVLEVLREPLESGQVVISRAAKQMTFPA
                     AFQLIAAMNPCPCGFLGDGSPRCRCTPEQIARYRGKLSGPLLDRIDLHIEVPALAASV
                     LRDNQVSESSAQVKERVLAARERQQHRQQCTNKHLTPRLLDQYCILDEKEGRLLDQAM
                     QRLRLSARAYHRILRVARTIADLANSEQILVPHITEAIGYRLLDRQ"
     gene            complement(3774..>6442)
                     /locus_tag="PLY05_04835"
     CDS             complement(3774..>6442)
                     /locus_tag="PLY05_04835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010591457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02662.1"
                     /translation="HLPLIDLQNLISHAECYWHDSKIPLSYSQTSYQLQLKWQQDTQQ
                     GERLVATLQAKDQTSPIPDKFEVLYTHPLAYLDTEQCIIGQLECEYASHVIRQLLAMP
                     NIPKALIPKVSEVLQSSPLTQHISQPNSLKNIEDIYGTLTPIIRFGGFNKIADYLFHQ
                     GLGLAEIYFDYIGGTIRATEKNSYFFGTDNGEIVRQHRDLSQEKQRVSQLQKLIPSLQ
                     WLEKNKSIQALTHNQEHALILAQNTDFIKNIIPTNKFAELGWQVEHLADSPFNVQLGH
                     NINLSLTESSSQQDWFELGATIQDPQGNTFNLVDLLSRLIKSHPFLLDAASLEELQED
                     DFLSVYLGENQPALALLIKDIKPILLHLGHLLNQDSHGMDKYDAAQALELQHVLGMPW
                     QNNERLQQFANKLKQGYSQQLPTPQGFVGELRPYQQQGLGWLQFLRDTEHGGILADDM
                     GLGKTAQTLAHILLEKQAGHLQQKPALIIAPTSLMYNWLKEAQKFTPDLKVLVLQGTN
                     RHQDFDKIPQHDIVLSTYPLLARDEELLKQHQYHLLILDEAQNIKNPRAKAAQVVRQL
                     DAKHRLCLTGTPMENHLGELWSLFYFLMPGFLSSQELFNKNYRHPIEKKGDNQLRQKL
                     VNRIKPFMMRRLKTEVAKELPAKTTIEINVDMSESQSKLYEAVRVAMQANIQDIIAKQ
                     GFKRSQIAILDALLKLRQVCCHPSLLKLDKMPSDKASSAKLENLIEMVTEMVEEGRKI
                     LIFSQFTTMLQLIEDNLNSQKIAFVKLTGQTKKREEVINAFQTGQVPVFLISLKAGGV
                     GLNLTAADTVIHYDPWWNPAAEDQASDRAWRIGQDKPVFVYKLITNQSIEEKIIALQK
                     NKADLAASILSHDKEHQVKLSEEDVLQLFERF"
BASE COUNT         1810 a         1214 c         1351 g         1967 t
ORIGIN      
        1 aagtaattgc ggcaaaagca ccttggaaca cgatataaat catttcgtga attggggttg
       61 ctttgctcca tgttgctgct aaagaatcag gagtgacgcc actgaggaat actttagaga
      121 gtgaaccaaa aaaggcatta ccttcagtaa acgcgatact gtagccgtaa atcatccata
      181 acacgctaat tagcgaaaat acggtaaata cttgcattaa taccgaaagc atattctttt
      241 tacgcactaa accgccgtaa aataatgcta agccgggaat tgacattaaa ataacaaagg
      301 cagagcaaat cattaagaat gcatcatcac ctttatttgc agtaggggca ggagcagcgg
      361 cagcaggagc tgcttcggca ggtgctgcaa caggagcagc ttcgggacta ggagcaggag
      421 ttgcactggc ttcaacggta gcagtagcaa caggagcagc cgcaggtgca gcagactctt
      481 ctgcatagag tggtgttgca ccaagcagtg cgccactcaa actaagggca agaattatct
      541 tcttcacgat ggtttctcca tgtgagtgcc aagggggagt aaaaataagg acttgtgcct
      601 aagggaattt ttaaattgcg cgagtcctag aattgggctt aaacagcgtc aacacccgtc
      661 tcgccagtgc gaatacgaat aatctgttct aaggaggtga caaaaatttt gccatcgcca
      721 attttgcccg tatttgcggc tttggtaatt gcctcaatta cgcggtcaac taaatcatca
      781 ccgattgcca cttcgatttt gactttaggt aaaaaatcaa ccacatattc tgcgccacga
      841 taaagttcgg tatgaccttt ttgacggcca aaacctttca cttccgtaac ggtaataccc
      901 tgcacgccaa tgtcagataa gctctcgcga acatcatcta acttaaaggg tttaataatg
      961 gcggtgacta atttcatgtt taattcctca aaaataaggg cttaccttgt gagtaagccc
     1021 tatgatgtta gaaaccttta gttaaggtta aaacaaagcg gccttcgcat aagcctgcgc
     1081 cacattcttt ttcgtcaatg ttagagccga tatatgcgcc ttcaacagta ataccactaa
     1141 ttgctttaga agcagctaat ttatagtcaa tgtagctgtc atcactgcct gttgtgccta
     1201 atgcttggct catcatagcg gcactgtcaa atttgttcat gccaacactg ccaaacaaac
     1261 ctacaccagc aacctctgta ccataacttg catagaggta taaaaatttg tctgattcaa
     1321 agaaatagtc tgggctgtag ttaaaaccaa cttttgcacc catagtacta acagaggcgt
     1381 aaacttcgtt gtagtcagct tcaaatgctg cattattatc atttaaacct ggatagttat
     1441 aacgcatcac acctacgtca taagccactt cacctgcttt accacnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnngagct tgcaagtaag gctttggcta aagaagaaag
     1621 tttcatagtg accccattgt ctattattga aaattaacgt tcgctacctt aaaagggttt
     1681 gtcttatctc taagttatga caaactaacc ttagaattgg tagcaactgt gcaagatata
     1741 atgcagaact cgtgccaaga tggattttgt aggtggttaa aggtgttaca gagtgtcttt
     1801 gcacaaattt gaatatttct tttgaagcct gtgttgtatt tatgatctat tttggtgcgc
     1861 tattttggtg ctttttttat tttggtgcgt tttttaggcg aggttgatat ggtattagat
     1921 aatttggtgc aaaaagttcg agaacaggtg cgtgaccgct ttgagcaagc gaaccaaaca
     1981 ggcgcatggc aagaattaga taaaaattta caatccgtat tacaagaagt ctttgccaaa
     2041 atggagttag tgacgcgtga ggaatttgat cgtcaagcac aattattagg tgaggcacga
     2101 atgaagttac aaatgctaga aaaacagcta aaatcactcg aaaatcaaat taaatagttt
     2161 ttatcaagga taaatttagt tttatcaaat taacagggat gtcatcatgt ctttagcttt
     2221 agtctatagt cgtgccgctt tgggtttgca cgcgcctctg gttactgtcg aagttcatat
     2281 tagcaatggt ttacctgctt taacgattgt gggtttacct gaagcggctg tgcgtgaaag
     2341 taaagatcgt gttcggtcag cattaattaa tgcaggtttt gaatttcctc aacggcgagt
     2401 gacgattaat ttagctcctg ccgatttgcc caaagagggt ggtcgttttg atttgccgat
     2461 tgccttgggt attttggccg ccagtggcca aataatcact gagagcttgc aacataaaga
     2521 gtttgtgggt gagctggcat taacaggcga attaaggccg attattggtg tattgtctgc
     2581 aagtttggca gcaactaaag aaggacattc tttatatgtg cctgtggata atgctcaaga
     2641 ggcggcctat ccgcagcaag ctcaaattta tggcattcgt catttgaaag agttgtgtgc
     2701 tagtttggct ggtgacgttg tgttaacagc cacaaaaaat caaactcaac atcatcatgt
     2761 caaatcaccc gatttaagtg atgttaaagg tcaacatcgt gcgcgtcgag ctttagaaat
     2821 tgcggcggct ggtggccatt ctttattaat gatgggcccc ccaggaacag gcaaaacctt
     2881 attagcatca cgtttatcag gtattttgcc tcctttagac gataatcaaa gtattgaagt
     2941 tgccactatt cattccgtag cagcacaaaa ttatccgcat tttggtttgc cgccgtttcg
     3001 tgcgccacat cataccgctt ctgcacctgc cttagtgggt ggtggttcta atcccaaacc
     3061 tggtgaaatc agtttggcac atcatggtgt attattttta gatgagttac ctgagtttga
     3121 tcgaaaagtt ttagaggtgt tgcgcgaacc tttagagtct ggtcaagtag tgatttcacg
     3181 tgctgctaaa caaatgactt ttcctgcggc ttttcaatta atagcggcga tgaatccttg
     3241 tccttgtggg tttttaggcg atggttcgcc gcgttgtcgt tgtacacctg agcaaattgc
     3301 tcgttatcgc ggcaaattat caggcccttt attagatcga attgatttgc atatcgaagt
     3361 ccctgcattg gcagccagtg tgttgcgcga taatcaagtg agcgaaagtt cggctcaagt
     3421 taaagagcgt gttttggcgg ctcgtgagcg gcaacaacac cgccaacaat gcaccaataa
     3481 acatttaaca cctcgtttat tagaccaata ttgtattttg gatgaaaaag aaggacggtt
     3541 attagatcaa gcgatgcaac gtttaagact ttctgcgcgg gcatatcatc gaattttgcg
     3601 tgtggcacgt acgattgctg atttggcaaa cagtgagcaa attttagtgc cacatattac
     3661 cgaagccatt ggttatagat tgttggatag gcagtaagct atcattaata atcacgttag
     3721 gttttttgtt gttactttgc ctatttgcag acaaagtgac aacagccgca cacctagaag
     3781 cgttcaaata actgcaatac atcttcttct gatagtttaa cctgatgttc tttatcatgg
     3841 cttaaaatag acgcagctaa atcggctttg tttttttgta aagcgataat tttttcttca
     3901 atgctttgat tagtaatcag tttataaaca aatacaggtt tatcttggcc aatacgccat
     3961 gcgcggtcag aggcttgatc ttcagcagca ggattccacc acggatcata atgaatcact
     4021 gtatccgctg cggttaaatt aagaccaaca ccacccgctt ttaaactaat taaaaacacg
     4081 ggtacttgtc cagtttgaaa agcattaatc acttcctcac gtttttttgt ttgacctgtt
     4141 aatttaacaa aagcaatttt ttggctgttt aggttatctt caatcagttg caacatagtg
     4201 gtaaattgcg aaaaaatcag aatttttcgg ccttcttcca ccatctcggt gaccatttcg
     4261 attaagtttt ctagcttagc ggaactggct ttatcggatg gcattttgtc gagttttaat
     4321 aaactggggt gacaacacac ttggcgcagt tttaataagg catctaaaat agcaatttgg
     4381 ctacgcttaa atccttgttt ggcaataata tcttgaatat tagcttgcat ggccacacgc
     4441 accgcttcat acagtttaga ctgtgattca ctcatatcga cattgatttc gatagtcgtt
     4501 ttggcaggta attctttggc aacctcagtt tttagacggc gcatcataaa cggtttaata
     4561 cgattaacca gtttttgtcg taattgattg tcgccctttt tttcgatagg gtggcgatag
     4621 tttttattaa ataattcttg gctcgataaa aaacctggca ttaaaaaata gaataatgac
     4681 cacaattctc ctaaatggtt ttccatgggt gtgcctgtta aacacagccg atgtttggca
     4741 tctaactgac gtaccacctg tgcagcttta gcacgcgggt ttttaatatt ttgtgcctca
     4801 tctaaaatta acaaatgata ttgatgttgc tttaacagtt cttcatcacg ggctaacaat
     4861 gggtaagtgc ttaacacaat atcgtgttgg ggaattttat caaaatcttg atgacgattt
     4921 gtgccttgta atactaacac ctttaaatca ggggtaaatt tttgggcttc tttaagccag
     4981 ttatacatta acgaggtggg cgcaataatc aaggcaggtt tttgctgtaa atgacctgct
     5041 tgtttttcga gtaaaatatg cgctaaggtt tgagcagttt tgcccaaacc catatcatca
     5101 gccaaaatgc caccatgttc tgtgtcgcgt aaaaactgta gccaacccaa accttgttgt
     5161 tgataaggac gtaactcacc cacaaaacct tgaggtgtag gcaattgttg gctataacct
     5221 tgtttgagct tgttggcaaa ttgttgtaaa cgctcattat tttgccacgg catacccaat
     5281 acatgttgta gctcaagggc ttgtgcggca tcgtatttat ccatgccatg actatcttgg
     5341 ttaagcaaat ggccaagatg cagtaaaata ggttttatat ctttgataag taacgctaag
     5401 gctggttgat tttcacctag ataaacactt aaaaaatcgt cttcttgtaa ttcttcaagc
     5461 gaggcagcgt caagcaaaaa aggatgactt ttaattaatc gacttaacaa atcaactaaa
     5521 ttaaaggtat tgccttgtgg gtcttgaatc gttgcgccta actcaaacca atcttgctgt
     5581 gagctagatt cggtcagtga taagttgata ttatgaccca attgaacatt aaaaggactg
     5641 tcagccagat gttcaacttg ccaacccaat tctgcaaatt tgttggtggg aataatgttt
     5701 ttaataaagt cggtattttg agctaaaatt aaagcatgct cttggttgtg agtaagtgct
     5761 tgaatgctct tattcttttc aagccactgt aaagacggaa ttagtttttg taattggcta
     5821 acacgctgtt tttcttgaga taaatcacgg tgttgacgta ctatttcgcc attgtctgta
     5881 ccaaaaaaat aactgttttt ttcggtcgct ctaatagtcc cacctatata gtcaaaataa
     5941 atttcggcca agcctagccc ttgatgaaat aaataatctg caatcttgtt aaaaccacca
     6001 aaacggataa tcggtgttaa tgtgccataa atatcttcga tgttttttag agagtttggt
     6061 tgagagatat gttgggtaag aggcgagctt tgtaagactt cactgacttt tggaatcaga
     6121 gctttaggaa tgttaggcat tgctaataat tgacgaatga cgtggctggc atattcgcac
     6181 tcaagttggc caatgatgca ctgctcggta tcaagatagg ctaaggggtg ggtataaagt
     6241 acctcaaatt tatcaggaat gggtgaggtt tggtctttag cttgtagagt agcgactaat
     6301 ctttcgcctt gttgtgtatc ttgctgccat ttaagctgta attgataact ggtttgtgaa
     6361 tagcttaaag ggattttact atcatgccaa taacattccg catggctgat aaggttttgt
     6421 aaatctatca atggtagatg gt
//
LOCUS       DAOQMB010000093         6441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5076_length_6441_cov_7.161134, whole genome shotgun sequence.
ACCESSION   DAOQMB010000093 DAOQMB010000000
VERSION     DAOQMB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5076_length_6441_cov_7.161134"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1386)
                     /gene="pglX"
                     /locus_tag="PLY05_04840"
     CDS             complement(<1..>1386)
                     /gene="pglX"
                     /locus_tag="PLY05_04840"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HNC02663.1"
                     /translation="WFNRFCALRFMDVNGYNSIRMVSPLDDQTQPEILAEAKMGHIDE
                     RFVPQQAIQQKIYALLEGRLPSHAPEQEAYRLLLVAACNYWQPAMPFMFERIADYTEL
                     LMPDDLLSANSILTATREAMTAEACQDVEVIGWLYQFYISEKKDQVFDALKKNKKIEA
                     NDIPAATQLFTPHWIVRYLVENSLGRLWLLNHPESKLAEKMPYYIAPTQPETDFLVIT
                     SPEELKVCDPACGSGHMLTYAFDLLYAIYEEALYEPSQIPSLILSKNLFGIEIDERAA
                     ALAAFALTMKARAKQSRFLAKATKTLPNICHLQPVKFEPRELEQYMDLQGKALFTDEI
                     KTLFTQFEEADNFGSLIRPVELKNSELRTTLQQNGVDGHLLLEHVHEDVLRVLKQAEF
                     LQQRYQVVVANPPYMGGKGMNGRLAEWAKSNYPDSKSDLFAMFMERGFELVPKHGYSA
                     MVTMQSWMFLSS"
     assembly_gap    1387..1486
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1488..>3688)
                     /gene="brxC"
                     /locus_tag="PLY05_04845"
     CDS             complement(1488..>3688)
                     /gene="brxC"
                     /locus_tag="PLY05_04845"
                     /inference="COORDINATES: protein motif:HMM:NF033441.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HNC02664.1"
                     /translation="EGIRTALKSNIQRAILQAEQHLGDAFAVKVLKTLFLVKYVKDFK
                     STLRNLSVLMLEEFNQDIASHRQRLEHALNLLEQQTYIQRHGEFYEYLTDTEKDVETE
                     IKNTDVDINDVVNELQKIVFDQIIKQTKIRYSNQQDYNFTRKMDDRLYSREYELAIHI
                     ISPFNENAANNTNLRMQSMGRDELRVIMPADARLMNDLLMYKRTEKYVRQNTSLVQKD
                     GIQQILGSKALQNQERLAEISQRIQRLVGESSLIINGGDIDISSTDGQSRIISGFHQL
                     IEYTYTNLSMLRSINYSENDIGKYLNKAKDGLLANDVAPLTEAEQEILAFIKANQRNG
                     GRTTIKAVIDKFERKNYGWYYAAILCNLALLASRGKIELRSDGNLLEDAAIERALRNT
                     QSQNNVIIDPQIEFSPSQIRALKEFYNEFFDKPAPASEAKLLAKETAEAFKSLFIDLD
                     KLHSQKAHYPFLATLSAVLDKIKPISQQQSAWFFTELAKQEEALFELKENMTAPVLSF
                     MNGAAKSLYDQANAFLSQQNANLNALDATEIQAIKLALADTLIYQGNKIQRISQQLDG
                     LKTKLKQLHTQELEQAVAKIEQLRQKLRQSPEFALLNNAQQQELMQTFEQSIAQLGQQ
                     QLIAVIRDNCRSFEEKTFIQLSDKLHDWLQPKPMPVEPITDKKEVQSSVDNKVAEKKP
                     EYVKPSIASRDIKVNFAKPWLLSESDVDDYLKAMRDALLNEINTGKRINL"
     assembly_gap    3689..3788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3789..4273)
                     /locus_tag="PLY05_04850"
     CDS             complement(<3789..4273)
                     /locus_tag="PLY05_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HNC02665.1"
                     /translation="MDNTQLLKDIFLKPVDRPIDGVIKADDQASLRLELEEYVLTNEV
                     AQRLNEFLIAYANYHGANGVWVSGFFGSGKSHLLKMLAFLLENRVVNGTPTLDFFLPK
                     CREQDKSDAILEANLKKAVAIPSKSILFNIDQKADVISKTQIDALLSVFVKVFDEMCG
                     Y"
     gene            complement(4307..4900)
                     /locus_tag="PLY05_04855"
     CDS             complement(4307..4900)
                     /locus_tag="PLY05_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011784100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HNC02666.1"
                     /translation="MVSIASRYEHLLAVISSDRFLQMQGLGNEVPFFICPFKPSDAVA
                     MGKLQHQLINKLEQLGVKALEINLYDLAIDLIKARGLWDRILELEPTLAKDALKELLQ
                     NILDPEEHLMPEIAQRLGAEQHDVLFITGVGEVFPYIRSHNVLNNLQKEAKDKPTVMF
                     FSGGYTHSLASGAALVLFGRLQDDKYYRAFNIDDYQI"
     gene            complement(4888..5505)
                     /locus_tag="PLY05_04860"
     CDS             complement(4888..5505)
                     /locus_tag="PLY05_04860"
                     /inference="COORDINATES: protein motif:HMM:NF020425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HNC02667.1"
                     /translation="MNNQTKYSLSFTTGSLFAKESVIFAELYLKLADWQLVRDEVRNH
                     NLLQQRTLVSARKITASVIKRLKCLSHDELSYLVDATPLERGYLLWLAACLDYSFIGD
                     FAVEVVNEYFIQLKPQLSYDDFDIFFNQKTLWHDELNHITDSTRLRLRTNLFKMLKES
                     GLLSSQNTLQSVLLSPPLATLLSKRHISAFEIFPIFHRDIQTWLA"
     gene            complement(5550..>6441)
                     /locus_tag="PLY05_04865"
     CDS             complement(5550..>6441)
                     /locus_tag="PLY05_04865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HNC02668.1"
                     /translation="ISNEVIKDFVWRRRWRLVTWVGVSIFAMLAVVKYFLAYGLPTFG
                     NQIAVIDINGEIGQGYIASADKVVPVLRNAFQNEDVKVVILEINSGGGSPSEAERITS
                     EIDRLKKKHPKPIISVIGSLGASAAYMIAVHTDKVVAGKYSLVGSIGVIMQGYDAHKL
                     AERLEVKQRVYASGPFKNLMSPLHEPTQAEQKVLQTLVDNAAQAFIEEVKLKRGKKLT
                     RDDIYTGEVWNGLDAVKFGLIDSIGTLESVTSEYGDLKVSNFGPNLNSPFSANFAHEL
                     GAGFAEGVITAGKQQIEYVK"
BASE COUNT         1782 a         1308 c         1216 g         1935 t
ORIGIN      
        1 gaagataaaa acatccaact ttgcatcgtc accattgcac tataaccatg cttaggaaca
       61 agctcaaaac cacgctccat aaacatcgca aacaaatcag acttactatc ggggtaattg
      121 ctcttagccc attcagctaa tctcccattc atccctttcc cacccatata cggcggatta
      181 gccaccacca cctgataacg ctgctgcaaa aactccgctt gcttcaacac ccgcaacaca
      241 tcctcatgca catgctctaa caacaaatga ccatcaaccc cattttgctg taaagtcgtg
      301 cgtaactcac tgttttttaa ctcaacaggc cgaattaacg aaccaaaatt atccgcctcc
      361 tcaaactgcg taaataaagt cttaatctca tcggtaaata acgccttacc ttgtaaatcc
      421 atatattgct caagttcgcg tggctcaaac tttacaggct gcaaatggca aatattgggc
      481 agcgtttttg tcgctttcgc taaaaagcgc gattgtttag cgcgtgcctt catggttaaa
      541 gcaaacgctg ctaatgctgc tgcacgctca tctatctcaa tgccaaataa atttttgctt
      601 aaaataagcg agggaatttg gcttggctca tataacgcct cttcataaat tgcatatagc
      661 aaatcaaaag cataagtcag catgtgtccc gacccacaag cagggtcaca cacttttaac
      721 tcttcgggcg aggtaatgac taaaaagtcg gtttcgggct gcgtaggcgc aatgtagtac
      781 ggcatttttt cggccagttt agactctgga tgattaagca accacaaacg acctaatgaa
      841 ttttctacca aataacgcac aatccaatgc ggtgtaaata attgcgttgc cgcaggaata
      901 tcattcgctt caattttttt gtttttcttg agtgcatcaa aaacttggtc ttttttctcg
      961 gaaatataaa actgatacaa ccaaccaatc acctctacat cctgacaggc ttcggcagtc
     1021 atcgcctcac gggttgccgt taatatcgaa ttggccgata acaaatcatc gggcatcagt
     1081 aattcggtat agtcggcaat acgctcaaac ataaaaggca tggcaggttg ccaataatta
     1141 caggcagcca ccaacaacaa acgataggct tcttgctctg gcgcgtggct tggtaagcgt
     1201 ccctctaaca aggcatagat tttttgctgt atggcttgtt gcggcacaaa acgctcgtca
     1261 atatgcccca ttttggcttc ggcaaggatt tcgggttggg tttggtcatc aagtggcgac
     1321 accatacgaa tgctgttata accattaaca tccataaaac gtaaagcaca aaaacggtta
     1381 aaccatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttta caagttaatc
     1501 cgtttacccg tgttaatttc gttaagtaag gcatcacgca tggctttaag gtaatcgtca
     1561 acatcggact cactcagcaa ccacggtttt gcaaaattga cttttatatc ccgactagca
     1621 attgacggtt tgacatattc gggctttttt tcggctacct tattatcaac agaagactga
     1681 acctcttttt tgtctgttat aggttctaca ggcataggtt tgggctgtaa ccagtcatgc
     1741 agtttatccg ataactgaat aaaggtcttt tcttcaaagc tacggcaatt atctctaatc
     1801 acggctatta gctgttgctg acctaattga gcgatacttt gctcaaaagt ttgcatgagt
     1861 tcttgctgtt gggcattgtt gagtagggca aactcaggcg actgacgcaa tttttggcga
     1921 agctgttcta ttttagcaac cgcttgctca agttcttggg tgtgcaattg cttaagtttg
     1981 gttttgaggc catcgagctg ttggctaata cgctgtattt tgttgccttg ataaataagg
     2041 gtatcggcta aggcaagttt aatagcctgt atttctgtag catctagggc gtttaaattg
     2101 gcattttgct gagataaaaa ggcgtttgct tggtcataca aacttttagc tgcgccattc
     2161 ataaaactta atacaggcgc ggtcatgttt tcttttaact caaacaaggc ttcttcttgc
     2221 ttggctagtt cggtaaaaaa ccatgcgctt tgttgttggc taatgggttt aattttgtct
     2281 aaaaccgcgc ttaatgtggc taaaaaggga tagtgagctt tttggctgtg cagtttgtct
     2341 aagtcaataa atagagattt aaaggcttct gctgtttctt tggctaagag tttggcttca
     2401 ctggcagggg ctggcttatc aaaaaattcg ttataaaatt ctttgagtgc gcgaatttga
     2461 gacgggctaa attcgatttg agggtcaata atgacgttgt tttgactttg tgtgttgcgt
     2521 aaggcgcgtt cgattgcagc atcttcgagc aagttgccat cgctgcggag ttctattttg
     2581 ccacgggacg ctaataaagc aaggttgcat aaaattgccg cgtagtacca gccgtagttt
     2641 ttacgctcaa acttgtcgat gaccgcttta atagtggtgc gtccaccatt acgttgatta
     2701 gctttgataa aggctaaaat ttcttgttcg gcttcggtta atggagcaac atcgttagcc
     2761 aataaaccgt ctttagcttt attgaggtat ttgccaatat cgttttcgct atagttgata
     2821 ctacgcaaca tactgaggtt ggtataagtg tattcaatca attgatgaaa gcccgatatg
     2881 atgcggcttt gaccatcggt tgaactaatg tcgatatctc caccattgat aatcaagcta
     2941 gattcgccca ctaaacgttg tatccgttgc gaaatttcgg ctaaacgctc ttggttttgt
     3001 agggctttgc tgcctaaaat ttgttgaatg ccgtcttttt gcactaaaga ggtattttga
     3061 cgaacgtatt tttcggtacg tttatacatc agtaaatcat tcatcaaacg ggcatcggca
     3121 ggcattatca cccgtagttc atcacgtccc atgctttgca tccgcaaatt ggtattgttt
     3181 gcggcatttt cgttaaaagg actaataatg tgaatggcta actcatattc tcggctatac
     3241 aaacgatcat ccattttgcg cgtaaaatta taatcttgtt gattgctata gcgaattttg
     3301 gtttgcttga taatttggtc aaagacgatt ttttgcagct cattcaccac gtcattgatg
     3361 tcaacatcgg tgttttttat ttctgtttca acgtcttttt cggtatcagt taggtactca
     3421 taaaactcac catgacgctg aatataggtt tgttgttcta gtaagtttaa ggcgtgttcc
     3481 agacgttggc gatggctagc aatgtcttgg ttaaactctt ccagcattaa cacactaaga
     3541 ttgcgtaagg tggatttaaa gtctttaaca tatttgacca aaaataaggt ttttaatact
     3601 ttaacggcaa atgcgtcgcc aaggtgttgt tctgcttgca aaatcgcccg ttgaatgttg
     3661 gatttaagcg cggtacgaat gccttcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnta gtaaccacac atctcatcaa aaactttgac aaacaccgag agtagggcat
     3841 ctatctgggt tttgctaatg acatcggctt tttggtcaat attaaataaa atacttttag
     3901 acggaatggc caccgctttt ttcaggttgg cttctaaaat ggcatcgctt ttatcttgct
     3961 cacggcattt tggtaaaaaa aagtcgaggg taggcgtacc attcactaca cggttttcca
     4021 gtaaaaatgc taacattttt aataaatgcg acttacccga cccgaagaaa cccgataccc
     4081 atacgccatt ggctccatga taattagcat acgctattaa aaactcatta aggcgttggg
     4141 cgacttcatt ggttagtaca tactcttcga gttcaaggcg taaactggct tggtcatcgg
     4201 ctttaattac gccatcaatc gggcgatcta caggctttaa aaaaatatct tttaataatt
     4261 gagtattgtc cataaaagta ttaactctac atctaacatt cgttgtttaa atttgataat
     4321 cgtcaatgtt gaaggcgcga taatatttat cgtcttggag tcgtccaaac aacacgagag
     4381 ccgcaccact ggctaaagaa tgggtatagc cacccgaaaa aaacatcacc gttggtttgt
     4441 ctttggcttc tttttgcaga ttatttaata cattgtgcga gcgtatatac ggaaagactt
     4501 caccaacacc cgtgataaat aacacatcat gctgttctgc gccaagacgt tgagcaattt
     4561 cgggcattaa atgctcttcg gggtcgagaa tgttttgtaa aagctctttt agggcatctt
     4621 tggcgagggt tggctctagc tctaaaatac gatcccataa gcctcgtgct ttaatcaaat
     4681 caatggctaa atcataaagg ttaatctcta aggctttgac tccgagctgt tccaacttgt
     4741 tgatgagctg gtgctgtaat ttgcccattg caacggcatc gcttggctta aagggacaaa
     4801 tgaagaaggg gacttcgtta cctaagcctt gcatttgcaa aaagcggtcg ctgctaataa
     4861 cagccagtag gtgttcatac cgtgaggcta tgctaaccat gtttgtatgt ccctgtgaaa
     4921 aatcggaaat atttcaaaag cggatatatg gcgtttactg agtaaagtcg ctaacggagg
     4981 cgatagtaaa acgctttgta aggtgttttg agacgacaat aggccactct ctttgagcat
     5041 tttaaacaaa ttggtgcgta agcgtagccg tgttgagtcg gtgatgtgat tgagttcgtc
     5101 atgccaaagt gttttttgat taaaaaatat atcaaaatcg tcgtagctga gttgtggctt
     5161 taattgaata aagtattcat taactacttc aaccgcaaaa tcaccaataa acgaatagtc
     5221 caaacaggcg gctaaccaca gtaaataacc acgctctaaa ggtgtagcgt caactaagta
     5281 gctcaattcg tcatgactta ggcatttaag tcgcttaatc acactcgcgg taattttacg
     5341 agccgaaacg agagtacgtt gctgtaataa gttgtgatta cgaacttcat cacgcactaa
     5401 ttgccaatca gctagtttta aatataactc agcaaaaatc actgactctt tggcaaataa
     5461 actgcctgta gtgaatgata aactgtattt ggtttgatta ttcatggtca gcgcatacga
     5521 ttgattataa agaaaatatt ttagttatct cattttacat attcaatttg ctgttttcct
     5581 gcagtgataa caccttcggc aaagccagct cctaactcat gagcaaaatt agcggaaaaa
     5641 ggtgaattca gatttgggcc aaaattacta acttttaaat caccatactc acttgttact
     5701 gattctaaag taccaatact atcaataaga ccaaatttaa cggcatctaa accattccat
     5761 acttcgccag tataaatatc atctctggtc aattttttcc ctctttttaa cttcacttcc
     5821 tcaataaatg cttgagctgc attatcgaca agcgtttgta atactttttg ctctgcttgt
     5881 gttggttcgt gcaatggact cattagattc ttgaatgggc cagaagcata aacgcgctgt
     5941 ttaacctcca aacgctctgc taatttatga gcgtcatatc cttgcataat gacaccaata
     6001 ctgccaacta aactatattt accagcaacc actttatctg tgtgtacagc aatcatataa
     6061 gcagcacttg cacctaaact accaatcact gaaataattg gttttgggtg ttttttcttg
     6121 agtctgtcaa tttctgaggt aattctttca gcttctgagg gtgagccacc gccactgtta
     6181 atttctaaga tgaccacttt tacatcttca ttttgaaaag catttcttaa aacaggcaca
     6241 actttgtctg cagatgcaat atatccttgt ccgatttcac catttatatc aatgaccgct
     6301 atttgattac caaatgtagg taaaccatag gctaaaaaat atttaacaac agctaacatc
     6361 gcaaaaatac taaccccaac ccaagtgact aaacgccaac gccttctcca aacaaaatcc
     6421 tttatgactt cattagaaat t
//
LOCUS       DAOQMB010000094         6363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5178_length_6363_cov_15.197051, whole genome shotgun sequence.
ACCESSION   DAOQMB010000094 DAOQMB010000000
VERSION     DAOQMB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5178_length_6363_cov_15.197051"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..176
                     /locus_tag="PLY05_04870"
     CDS             <1..176
                     /locus_tag="PLY05_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4172 domain-containing protein"
                     /protein_id="HNC02669.1"
                     /translation="KGFELGISASQYQKVAKVSKATATRHLADLVAKGCVVKLEGGGR
                     NTRYQINTKLGVN"
     gene            260..952
                     /locus_tag="PLY05_04875"
     CDS             260..952
                     /locus_tag="PLY05_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02670.1"
                     /translation="MRALILISALLMSQLSYANKQLIGQWQGQVDGESFVIQFNADGT
                     GQIGQQAIYYQAQDNQLLVQSTEGILSYQWQLKGKQLIVQGGDLQGSLVLQRVSNNQN
                     VAKATAKNGSAQTPQQLVGKWCLASNFSANAGGGSSRSSCFTLQANGRYQYNQESSMD
                     AYGGGMWGGTSQQNQDSGRWSVNGQQIIAQSDNGQRYQYQLELKNHPKNRDPMICLDG
                     ECYVSYFQKAPW"
     gene            complement(959..>1414)
                     /gene="pdxH"
                     /locus_tag="PLY05_04880"
     CDS             complement(959..>1414)
                     /gene="pdxH"
                     /locus_tag="PLY05_04880"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00558.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HNC02671.1"
                     /translation="RMVLLKGVENQQFVFYSNYQSRKGEQIAANSYVALTFFWPELER
                     QVRIEGKINKVKAEISDTYFQSRPYSSRVGAWASEQSRPLDAKSTLVKRAAFFAAKYI
                     TSVPRPPHWGGYGITPERIEFWQGRPSRLHDRVVYSLGEHGWEKICLFP"
     assembly_gap    1415..1514
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1526..1876)
                     /locus_tag="PLY05_04885"
     CDS             complement(1526..1876)
                     /locus_tag="PLY05_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004644745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HNC02672.1"
                     /translation="MTWIITKYLITAGMVVLISETAKRSDKLGSLLAALPLVTVLTLV
                     WLYIEQQPSSKIANHAWYTFWYVIPTLPMFLAFPWLMSRFGFWPSLMMACLMTVICFV
                     IFALLVKRFGIELL"
     gene            complement(1963..>3240)
                     /locus_tag="PLY05_04890"
     CDS             complement(1963..>3240)
                     /locus_tag="PLY05_04890"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008306974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNC02673.1"
                     /translation="CIMGMARLNRPSIFVYGGTIKPGAGHTDIISVFEAVGQHAKGEL
                     NAIQVKQIEEVAIPGPGSCGGMYTANTMASAIEALGMSLPGSSAQNAVSDEKMSDCYR
                     AGQQVLELLKKDIKPSDIMTKKAFENSIRVVIALGGSTNAVLHFLAMAHTVGVDLTLD
                     DFVRLGETTPVLADLRPSGKYMMSELVAIGGIQPLMKRMLAQGLLHGDCLTVTGKTLA
                     ENLADVADYPEGQDIIRDFDQPIKADSHLIVLKGNLAPEGSMAKITGKEGLFFQGNAR
                     VYASEEDALQGILNGEVVAKDVIVIRYEGPKGGPGMREMLSPTSAVMGKGLGKEVALI
                     TDGRFSGGSHGFVVGHITPEAAEGGPIALIENGDVITIDAVTREISVNVSDEDMAKRR
                     ANWTAPAPKYTRGVLAKYAKTVSSASEGAVTDK"
     assembly_gap    3241..3340
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3648..4994)
                     /gene="argA"
                     /locus_tag="PLY05_04895"
     CDS             complement(3648..4994)
                     /gene="argA"
                     /locus_tag="PLY05_04895"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HNC02674.1"
                     /translation="MTDDFLSPDTMTEQYVHWFRNSAPYINAHRGKTFVLMFGGEAVN
                     HENFRNIIHDIALLHSLGIRLVLVHGARPQIDENLIEYGLSTPFHKGLRVTTRESLRC
                     VLDAVGSIRLEVEALLSMGLANSPMYGARIDAVSGNFVIAKPYGVREGVDFQLTGEVR
                     SIDTEAINKNLMNHHVVILGPTGYSTTGEVFNLLAEEVAVHAAIALKADKLILLGEKE
                     GIANDDDRLLREMIPNEVDQYLRGKVLDSEILRHMDASREACRGGVRRVHIISYARDG
                     ALLQELFTRDGSGTLITQDPYEEIRTAEIDDVVGLLELLRPLEEEGILVRRSRERLET
                     EINQFAVIERDGMIVGCAALYPLPADEQGVLTGEIACVAVHPSYRKGDRGSELLDYLE
                     ARARNKGIKKLFVLTTRTAHWFLEQGFNPVSVDDLPKERKALYNFQRNSQVFSKKL"
     gene            complement(5073..6224)
                     /gene="argE"
                     /locus_tag="PLY05_04900"
     CDS             complement(5073..6224)
                     /gene="argE"
                     /locus_tag="PLY05_04900"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HNC02675.1"
                     /translation="MDKLFLQQLSQLVALPSVSCTDPNIDQGNQAVIDLLASWLSQFG
                     FNCEIMPISVGKANLIATLGSGSGGLVLAGHTDTVPCDAQLWASDPFKVSERNGNLYG
                     LGIADMKGFFPAVLNAAQAFSKSKLKAPLIIIATCDEESSMAGAKALVEASKPQARFA
                     LIGEPTSLKPIRLHKGVMMERVKILGNSGHSSDPSLGNNALDAMTLVMSDLMAFRAEM
                     QKKYHNPAFTIPYPTLNLGCIHGGDNPNRICGQCELQFDLRPLPDMNMDDIRQQIAQR
                     LKRIALQQQIQIEYEAIFEGTPPAETPADSPFVKMVERLTQSESGSVAFGTEAPYFRQ
                     LGMDTIILGAGSIETAHQPNEYLPIKNISPLLLWLKQLIHHYCVLGYAA"
BASE COUNT         1896 a         1336 c         1245 g         1686 t
ORIGIN      
        1 aaaaaggctt tgaattaggc atcagtgcct cacaatatca aaaagtggct aaagtgagta
       61 aagccaccgc tactcgtcat ttagctgatt tagtggctaa aggttgcgta gtcaaattag
      121 agggaggagg gcgcaatacc cgttatcaaa ttaatactaa attgggcgtt aattgatatt
      181 gtcgtgctta cgatactttg ttgttgcata atccttagtg acaaacttta ctattagtat
      241 gactaaagga gtaagcatca tgcgagcttt aatcttaatc agtgccttgt taatgagtca
      301 gttaagttat gccaataagc aattaattgg acaatggcaa gggcaggtag atggcgaaag
      361 ttttgtgatt caatttaatg ccgatggcac aggtcaaatt ggtcaacaag cgatttatta
      421 tcaagcccaa gacaatcaat tattagtaca aagcacagag ggcattttaa gttatcaatg
      481 gcagctaaaa ggcaaacaac tgattgttca gggaggcgat ttacaaggca gtttagtctt
      541 gcagcgagtg agtaataatc aaaatgtagc aaaagcaaca gctaaaaacg gatcagcgca
      601 aacgccacaa caattagtgg gtaaatggtg tttagccagt aattttagcg ccaatgcagg
      661 tgggggcagc agccgaagta gttgttttac tttacaagcg aatggccgat atcaatataa
      721 ccaagaaagc agtatggatg cgtatggggg tggcatgtgg ggaggaacca gccaacaaaa
      781 tcaagattcg ggacgttggt cggtgaatgg tcaacaaatt attgctcagt cagataatgg
      841 ccaacgttat caatatcaac tggaattaaa aaatcaccca aaaaatcgtg atccaatgat
      901 ttgtttggat ggtgagtgtt atgtcagtta ttttcaaaaa gcaccttggt agttgttttt
      961 agggaaacaa acaaattttt tcccaaccat gttcgcctaa gctatacacc acacgatcat
     1021 gtaaacggct agggcgacct tgccaaaact ctatacgttc gggtgtaatg ccataaccgc
     1081 cccaatgcgg tggacgtggc acactggtaa tatattttgc tgcaaaaaaa gccgcacgtt
     1141 tgacgagggt tgattttgca tctaaaggac gactttgctc cgatgcccat gcaccaacgc
     1201 gtgagctata aggccgtgat tgaaaatatg tgtcagagat ttcagcttta actttattaa
     1261 ttttaccttc gatgcgtact tgtcgttcta attcgggcca aaaaaatgtt aaggcaacat
     1321 aactgttggc tgcaatttgc tcgcctttac gactttgata attgctataa aaaacaaact
     1381 gttgattttc tacgcctttt aataacacca tacgnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnttgttt tcgttttaca gtaattctat gccaaatcgt ttaactagca
     1561 aggcaaaaat gacaaaacaa atgacagtca tcaaacacgc catcattaaa cttggccaaa
     1621 agccaaaacg cgacatcaac caaggaaaag ctaaaaacat tggtaaagta ggaatcacat
     1681 accaaaaggt ataccaagca tgattggcga ttttgctact gggttgttgt tcaatataaa
     1741 gccaaaccaa ggtaagtaca gtcactaaag gcaatgcagc aagtagactg cccaatttat
     1801 cactacgttt agctgtttca gaaattaaaa ccaccatccc cgcagtgatt aaatatttag
     1861 tgataatcca agtcattgtt cactccaata aaaaaggcag agtattcact ctgccttacc
     1921 ctcaccctag acctctccca gtgggagagg gaatatcttg atttatttat cagttaccgc
     1981 accttcagaa gcactagaga cggttttggc atatttggcc aatacgccac gggtgtattt
     2041 gggtgcaggg gcagtccaat tagcacggcg ttttgccata tcttcatcgc tgacattaac
     2101 gctaatttcg cgcgtgacag catcaatcgt aatcacgtca ccattttcaa ttaacgcaat
     2161 tgggccacct tcagccgctt caggggtaat atggccaacc acaaaaccgt ggctaccgcc
     2221 agagaaacga ccatcagtaa tcaaggcaac ttctttgcct aaacctttac ccatcactgc
     2281 cgaagtgggt gacaacattt cgcgcatacc cggtccgcct ttaggacctt cgtagcgaat
     2341 gacgatcaca tctttagcaa ccacttcgcc gtttaaaata ccttgtaagg catcttcttc
     2401 actggcataa acacgggcat tgccttggaa aaataagcct tctttacctg tgattttagc
     2461 catagagcct tctggggcta aattaccttt taacacaatt aagtgagaat cagctttaat
     2521 aggttgatca aaatcacgga taatgtcttg gccttctgga taatcagcaa catcggctaa
     2581 gttttcagct aaggttttac ctgttacggt taaacaatca ccatgtaaca aaccttgcgc
     2641 caacatacgc ttcattaaag gttgaatacc gccaatcgca accaactcac tcatcatgta
     2701 tttgcctgat ggacgtaaat cagccaaaac tggcgtggtt tcacctaaac gcacaaagtc
     2761 atctaaggtt aaatcaacac ccaccgtatg cgccatagct aagaagtgta aaacagcatt
     2821 ggtagagcca cctaaggcaa tgaccacacg aatcgagttt tcaaaggctt ttttggtcat
     2881 aatgtcgcta ggcttgatgt cttttttcaa taattcaagc acttgttgac ctgcacggta
     2941 gcagtcagac attttttcgt cactcaccgc attttgtgcg ctagaaccag gtaagctcat
     3001 gcccaaggct tcaatggcag aggccatggt gttggctgtg tacataccac cacaagagcc
     3061 agggccggga atagccactt cttcaatttg tttgacttgg atggcattta attcaccttt
     3121 ggcgtgttgg ccaacggctt caaataccga aataatatcc gtgtggcctg caccaggttt
     3181 aatcgtgccg ccgtacacaa aaatagaggg gcgatttaaa cgtgccatgc ccataataca
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn atgtgcatat tacaaggcgt
     3361 taccattgcc catgtggatg caataccaat ttgtggtttg ttaaagtcat cgtcagtgaa
     3421 gcctacggcg cgtaacatgg cgcgggctgg tgcggcttca ataccatcaa caacttcgga
     3481 agaatgctta cgcatatttg gggtatcaca catactaaat ctcttgttaa aacgatatta
     3541 aatgaataat ggagcttgcc cttcgaatcc gctcagaaag tgctttacga ctccactcag
     3601 ggagtattct tgcgtgggct gagcgtagtc gaagcctaaa gggactatta taacttttta
     3661 ctaaacactt gcgaattgcg ttggaagtta taaagggctt tacgttcttt gggtaaatca
     3721 tcgaccgaca caggattaaa accttgctct aaaaaccagt gtgcggtgcg tgtagttaac
     3781 acaaatagct ttttaatgcc tttattacgc gcccgtgctt ctaaataatc taataactca
     3841 gaacctctat cgcctttgcg gtaactggga tgtacggcaa cacaggcaat ttctcccgtc
     3901 aacacgcctt gctcatcggc aggtaaggga tataaagcgg cacaaccgac aatcatgcca
     3961 tcacgttcaa tgaccgcaaa ttgattaatt tcggtttcta gacgttcgcg tgaacgacgc
     4021 accaaaatac cttcttcttc aagcgggcgt aataattcaa gcaagccaac gacatcgtca
     4081 atttctgcgg tacgaatttc ttcataaggg tcttgagtga ttaatgtacc tgaaccatcg
     4141 cgggtaaata actcttgtaa taatgcgcca tcacgcgcat aggaaataat atgtaccctc
     4201 cgcacaccac cacgacaagc ttcacgagaa gcatccatat gacgcaaaat ttcactatct
     4261 aagactttgc cgcgtaaata ttggtcaact tcattgggaa tcatttcacg caataaacga
     4321 tcatcatcat tggcaatgcc ttctttttcg cccaataaaa tcaatttgtc ggcttttaag
     4381 gcaatcgccg catgcaccgc gacttcttct gctaataaat taaagacttc accagtggtt
     4441 gaatagcctg ttgggcctaa aatgacaaca tgatgattca ttaggttttt attgatagct
     4501 tcggtatcaa tcgaacgcac ttcgcctgtt aattgaaagt caacgccttc acgcacgcca
     4561 taaggtttgg caatgacaaa attgcccgaa acggcatcaa tacgtgcacc gtacatcggt
     4621 gaattagcca agcccatgct taataaggct tcaacttcta aacgaattga tcccaccgca
     4681 tccaacacac aacgtaaaga ttcgcgggtg gtaacgcgta agcctttatg aaagggtgta
     4741 cttaaaccat attcaattaa gttttcatca atttgtggtc gtgcaccatg cactaacacc
     4801 aaacgaatac ccaaagaatg taataaggca atatcgtgaa taatattacg aaaattttcg
     4861 tgattaactg cttcaccacc aaacatcaac acaaaggttt tgccacgatg agcattaata
     4921 taaggggcag aatttctaaa ccaatgaaca tattgttcag tcatggtatc gggagacaaa
     4981 aaatcatcag tcataattat tactatccta aaaactaaac ttcgcagctg gcgataactc
     5041 cctctcccat tgggagaggg ctggggtgag ggctaagccg catagccaag tacacaatag
     5101 tggtgaatta attgttttaa ccacaataat aacggggaaa tgtttttaat gggtaaatat
     5161 tcattcggtt gatgcgcagt ttctatacta ccagcaccta aaataatagt atccatacca
     5221 agttgccgaa aataaggtgc ttctgtacca aaagccaccg agccagactc actttgggtc
     5281 aagcgttcaa ccattttgac aaagggggag tcagcaggtg tttcggcagg tggtgttccc
     5341 tcaaaaatgg cttcatattc aatttgaatt tgttgttgta gggcaatacg ttttaaacgt
     5401 tgagcaattt gttgacgaat atcgtccata ttcatatctg gtaatggccg caaatcaaat
     5461 tgcaactcac attggccaca aatacgatta gggttatctc caccatgaat acaacccaaa
     5521 tttaaggttg ggtagggaat agtaaaggcg ggattgtggt attttttctg catttcggca
     5581 cgaaaagcca ttaaatcgct catcaccaac gtcattgcat ctaaagcatt attgcccaaa
     5641 ctgggatcac tcgaatgacc actattgccc aaaattttta cccgctccat catcacccct
     5701 ttatgcaggc gaatcggttt taaactggtc ggctcaccaa ttagggcaaa acgtgcttga
     5761 ggtttgctag cttccactaa agcttttgca ccagccatag atgattcttc atcgcaagtt
     5821 gcaataataa tcagtggtgc tttgagtttg cttttgctaa aggcttgagc tgcatttaat
     5881 acagcaggga aaaaaccttt catatcggca atgcctaaac catacaaatt gccgttacgt
     5941 tcgcttactt taaaaggatc gcttgcccat aattgggcat cacaaggcac ggtatcggtg
     6001 tgccctgcca agactaagcc gccagaacca ctgcctaaag ttgcaattaa attagctttg
     6061 cctacactaa taggcataat ctcacagtta aaaccaaatt ggcttaacca tgaggctaat
     6121 aaatcaatca ccgcttgatt accttgatct atattagggt cggtacatga cacactaggc
     6181 aaggctacga gctgagaaag ttgttgtaaa aagagtttat ccatagttta acgagctaat
     6241 atttcagcaa aaaagtaaca cttggcagat gagtgtacat ggtatttact gagttttaag
     6301 tcttgtttaa aaaaaattac aactacatac tatttttatt agatcaaaat aacgcaagct
     6361 gct
//
LOCUS       DAOQMB010000095         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5222_length_6331_cov_7.688337, whole genome shotgun sequence.
ACCESSION   DAOQMB010000095 DAOQMB010000000
VERSION     DAOQMB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_6331_cov_7.688337"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(316..3195)
                     /locus_tag="PLY05_04905"
     CDS             complement(316..3195)
                     /locus_tag="PLY05_04905"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNC02676.1"
                     /translation="MSFFAKTPLPNAVNADASTLIPQAICDDILLEKYAKNEENSIFS
                     VRSRVAHALASTETKETQKTWQTQFLVAQEKGFIPAGRINSAAGTTLQATLINCFVQP
                     IGDSISETKDGKVGIYTALAQAAETMRRGGGVGYDFSSIRPMGAQVKGTQSRASGPVS
                     YMRVFDRSCETVESAGARRGAQMGVLRCDHPDIETFIHAKDSGDLANFNISVGVTDDF
                     MFAVESGSTIELLHKAEPNQEIKQQGAYVRDDGLWVYKKIPARTIWQQIMRSTYDHAE
                     PGILFLDRINRDNNLAYCETIEATNPCAEQPLPAYGCCCLGSINLTRFINHPFSAKAT
                     FDYDGFAQVIGVSVRMLDNVLDITHWPLPEQATEAANKRRIGLGFTGLGDALIMLGLA
                     YDSAQARDTASQISIFMRDKAYLSSVALAKEKGAFPLFDADKYLSGETFATHLPSDIK
                     KQIRQHGLRNSHLLSIAPTGTISLAFADNASNGIEPPFSWTYNRKKRMMDGSVKEYQV
                     EDYAWRLYKHLGHDVNNLPNYFVTALQISAQAHKDMVAAVAPYIDTAISKTVNIPENY
                     PYTDFEDLYVSAWRAGLKGLATYRPNAVLGSVLSVTDANKNPPNPEVEWVGANQRLSL
                     AHLPEPVLASLRWPNRPEFLEGNMAWTYLVNTPLGEFALFIGQTENEQQQALPFEVWV
                     NGTNQPRGLGAVAKTLSMDMRSNDNQWLKLKLDSLMRTVGEQSFELQFPPLGENKVVP
                     SVVSAFAQIVLYRCQKLGYFDTDSAHPVLDSMFSLHEPKTSTDGTLSWTVDIANPATG
                     EEFVLGLKEVVLAGGATRPYAMWLAGNYPRALDGLSRILSLDMRVIDPAWIGMKLRKL
                     LNYAEPLGDFLAFIPNTRRQCTWPSTVAYMARLIIHRYAMLGVLTEEGYPVDEMGILT
                     APISKITNPVIKTHGRQCQDCGNNAVIRKDGCDFCTVCGAIGSCG"
     gene            complement(3365..4006)
                     /locus_tag="PLY05_04910"
     CDS             complement(3365..4006)
                     /locus_tag="PLY05_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC02677.1"
                     /translation="MIRILLVDDHTLFRSGISALLASQDDIDVVGEAGEGSEALKLVQ
                     TLQPDIMLLDLNMPGLSGLEVLKLTLEDNPQQAVIMLTLSEESSDLLRALQLGAKGYL
                     LKNSNVDYLVNALRLVAQGGTAVHPDMTSYLVAGLRQLKKDETEEKEPLTPREKEVLQ
                     LVSTGQSNKEIARKLDIGESTVKFHIQSILRKLNLTSRVQAAVYASQHKHLSS"
     gene            complement(4028..5968)
                     /locus_tag="PLY05_04915"
     CDS             complement(4028..5968)
                     /locus_tag="PLY05_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNC02678.1"
                     /translation="MANIIDTVKTRLTAKITTMLLLALVFALMAIGYTLWLSWQLEGA
                     AAAINDSGSLRMRAWKIAAVVNKTPVGQTLSSTDIQDITQIRHAFEQTLAELHNGDPQ
                     RPLFLPRQTAIQAHLIQLDQDWQQHFAPQLDLLLTGKVFETYTAQDWQQQTAALVNKI
                     HSTVNLIEKEYARQTFWLRSSQMMLIVMALTGTLVMVILLHLMIVRPVTNLHQGLQQM
                     TTGDFTVRLPIEGQDEFSQLSVGFNAMADELQQLYADLERRVAEKTDSLEQKNRYLSV
                     LYDISQHINRHQSLEELCRNFLQRIVPEFGAAGGTVRLIDKNADRVYLVVDEGIPPSL
                     SQQMQCQKLGSCFCTTAAKLGKTAINNLDSHYSPHFSHCLNAGFKVVLAIPIQTADKN
                     QGIFNLHFSDNRKFNSEEITLIETLGQQLGIAIDNQRLTSHEKELAIVEERNLVAQGL
                     HDSIVQSLSFLNLQLQLLQNAVKQENLAQVAAAIPLLKTGVQQGYEDLRELMGNFRTR
                     YDDSQLEAAIGAVIRRFTEQSHIPVALTVDNSGQPLANDERLQILFILQEALSNIRKH
                     AQCSQVKVHVLNRNDFNLIIGDNGIGFLPNKVQSTTQKHIGLNIMQERASRIGAKFEI
                     NSVLNQGTTIKLRLTQEQRQKA"
BASE COUNT         1793 a         1300 c         1347 g         1891 t
ORIGIN      
        1 gtgacaggtg aggtgtcaaa ataaccccag cccccgtttt ggttcactct tctaaaacaa
       61 acacacaaag gcctgtctta tgcacaaggc aaagccaccg ctttaacgca aataaggaga
      121 gcgagagtga acggtaatcc tcctaaaaat aataggtttg aaaagtataa ttttctcgta
      181 agatatagga tattttgcat gaagacatcg aaattcagtg aaatccaaat cacggctatt
      241 cttaaacaag ctgaagtagg gaacctgcac cagtaagcga tgaactgtgt agcaggcttc
      301 ttttaaatat tgtctttagc cacaactgcc tattgcacca cacacggtac aaaaatcgca
      361 gccgtcttta cgaatgaccg cattattgcc acagtcttga cattggcgac catgcgtttt
      421 gataacagga ttggttattt tgctaatagg ggcagttaat attcccattt cgtccacagg
      481 atagccttct tcggttaaca cacctagcat agcgtaacga tgaataatca aacgtgccat
      541 ataagccact gtagatggcc atgtacattg gcgacgtgtg ttaggaataa acgccaaaaa
      601 atcacctaaa ggctcggcat aattaaggag tttgcgtagt ttcatgccaa tccatgcagg
      661 gtcaataacg cgcatatcta aagataaaat gcgcgataaa ccatctaagg cgcgcggata
      721 attgcctgct aaccacatgg cataaggacg tgttgcgcca cctgctaata ccacttcttt
      781 taagcctaat acaaattctt cgccagtggc agggttagca atatccactg tccatgacaa
      841 agtgccgtcg gtactggttt tgggttcatg caaactaaac atactgtcta agacagggtg
      901 agcggaatca gtatcaaaat aaccgagctt ttggcagcga taaagcacaa tttgagcaaa
      961 agccgagacc acgcttggta caactttatt ttcgcctaag ggcggaaatt gaagctcaaa
     1021 agattgttct cccacggtac gcattagact atctagtttt agtttgagcc attgattatc
     1081 gttgctacgc atatccatcg acaaggtttt ggccactgcc cccaaaccac gcggctgatt
     1141 agtgccatta acccatacct caaaagggag tgcttgctgt tgctcatttt cggtttggcc
     1201 aataaacagc gcaaattcac ccaatggggt attcaccaaa taagtccatg ccatattacc
     1261 ctctaaaaac tcggggcgat taggccaacg taaactggct aaaacaggct cgggcaggtg
     1321 cgctaaactt aaacgctgat ttgcgccgac ccattctact tctggattgg gaggattttt
     1381 gtttgcgtca gtcaccgata gcaccgaacc caaaacggca tttgggcgat aggttgccaa
     1441 gccttttaag cctgcgcgcc atgcactaac gtataagtct tcaaaatcgg tataggggta
     1501 attttcggga atattgaccg ttttagaaat cgcggtatca atatatggcg caaccgcagc
     1561 aaccatgtct ttgtgtgctt gtgccgaaat ctgtaatgca gtcacaaagt aattgggtaa
     1621 attatttaca tcatggccta aatgcttgta caaacgccac gcgtaatctt cgacttgata
     1681 ctctttaacg ctgccgtcca tcatgcgttt tttgcggtta tatgtccacg aaaagggtgg
     1741 ctcaataccg ttagaggcgt tgtcggcaaa agcgagcgaa atagtacccg ttggggcgat
     1801 agataacaaa tgtgagttgc gtaagccatg ttgacgaatt tgctttttta tatcactggg
     1861 taagtgtgta gcaaaagttt cgcccgataa gtatttgtca gcatcaaata gtggaaacgc
     1921 gcctttttct ttggctaaag caacagaaga taaatacgcc ttgtcacgca taaaaatact
     1981 aatttggctg gcggtgtctc gtgcttgggc agagtcatac gccaaaccaa gcataattaa
     2041 cgcgtctccc aagcctgtaa aacctaaacc aatacggcgt ttgttggcgg cttcggtagc
     2101 ttgttcgggc agtggccaat gggtaatatc taacacattg tctagcattc gcaccgatac
     2161 accaatcact tgagcaaaac catcataatc aaaagtggct ttggcactaa acggatgatt
     2221 aataaaacgt gtgaggttaa tagaacccaa gcaacaacag ccataggctg gaaggggttg
     2281 ctcagcacac ggatttgtcg cttcaatggt ttcgcaataa gctaaattat tatcacggtt
     2341 aatgcggtct aaaaacaaaa tacctggttc ggcatggtca taagtggagc gcataatttg
     2401 ttgccaaata gtacgcgcag gtattttttt atacacccat aagccatcgt ctcgtacata
     2461 agctccctgt tgtttaattt cttgattggg ttcggcttta tgaagtaatt caatggtact
     2521 gcctgactct accgcaaaca taaagtcatc ggtgactccc accgagatat taaaattggc
     2581 taaatcgcct gaatctttgg catgaataaa ggtttcgatg tcggggtggt cacaacgtaa
     2641 tacccccatt tgtgcgcccc gtcttgcgcc tgctgactct accgtttcgc aagaacggtc
     2701 aaacacacgc atataagaca cagggccact ggcgcgagat tgtgtacctt taacttgtgc
     2761 gcccatgggg cgaatactcg aaaagtcata gccaacaccg cctccacggc gcatggtttc
     2821 ggcggcttga gcaagtgcgg tataaatacc cactttgcca tctttggttt cgctaatcga
     2881 atcgccaatc ggttgcacaa aacagttaat taaagtggct tgtaaggttg tgcctgcggc
     2941 agagttaata cggccagcag gaataaaacc tttttcttgg gcgactaaaa actgagtttg
     3001 ccatgttttt tgcgtttctt tggtttcggt actggctaag gcgtgagcga cacgacttct
     3061 tactgaaaaa atagaatttt cttcattttt agcatatttt tcgagcaaaa tatcatcgca
     3121 aattgcttgc ggaatgaggg tggaagcgtc agcgttgaca gcgtttggaa gaggtgtttt
     3181 agcaaaaaag ctcatgattt ttatccttaa taatatatca aaacagtctt tgactataca
     3241 aaagataaga aaaatggaca aatttttaaa atgatgaaaa tcaacaaatt aacaatatat
     3301 agtgtttttt tataaatatg attctatata ttgtgttttt tgtggtttga attagatttt
     3361 tagactaaga agacaaatgt ttgtgttgac tggcatacac tgcagcttga acacggcttg
     3421 tcaaattgag tttgcgtaaa atactttgaa tatgaaactt aacggtgctt tcgccaatat
     3481 ccagtttgcg agctatttct ttattacttt gacctgtgga gactaattgt aaaacttcct
     3541 tctctcgcgg tgtcagcggc tctttctctt cggtttcatc ttttttgagt tgtcttaaac
     3601 ctgctaccaa ataactggtc atatcggggt gaacggctgt gccaccttgt gctactagac
     3661 gcaaggcatt gactaaataa tccacattgc tatttttaag taaataacct tttgcaccca
     3721 gttgtaaggc gcgtaataaa tcactagatt cttcggatag cgttagcata atgacggctt
     3781 gttgagggtt atcttctagg gtgagtttta atacttctaa acctgacaag ccaggcatat
     3841 taaggtcaag tagcataata tcgggctgta aagtttgcac taactttaat gcttctgagc
     3901 cttcgcctgc ttcgcccact acatcaatat cgtcttgact ggctaataaa gcactaatac
     3961 cacttctgaa taaggtatgg tcatcaacta acagaatacg aatcatgatt tactccaaat
     4021 ttttaattta ggctttttgg cgttgttctt gagtaagccg cagcttaatc gttgtgcctt
     4081 gatttaacac cgagttaatc tcaaattttg caccaatacg gctggcacgt tcttgcataa
     4141 tgtttaagcc tatgtgtttt tgtgtggttg actgtacctt gttaggtaaa aagccaatgc
     4201 cattgtcgcc aataattaaa ttaaagtcgt ttcgattaag tacatgaact tttacttggc
     4261 tacattgtgc atgtttgcga atattagaga gtgcctcttg taaaataaat aaaatttgta
     4321 gtcgttcatc attagctaag ggttgccctg aattatcaac ggtgagtgcg acaggtatat
     4381 ggctttgctc ggtaaaacgc ctaatcacag cacctatcgc agcctctagt tggctgtcat
     4441 catagcgagt tctaaaattc cccattaatt cgcgtaggtc ttcgtagccc tgttgtacgc
     4501 ctgtttttaa taaaggaata gccgctgcaa cttgggctaa attttcttgt ttgactgcat
     4561 tttgtaagag ttgtaattgt aaatttaaaa aactcaagct ttgtacgatg ctgtcatgta
     4621 ggccttgtgc caccaaatta cgctcttcaa caatggctaa ttctttttcg tgcgaggtga
     4681 ggcgttgatt atcaatggcg atgcctaatt gctgtcctaa ggtttctatt aacgtaattt
     4741 cttcggaatt aaacttccga ttatcactaa agtgtaaatt aaaaatgcct tgatttttgt
     4801 cggctgtttg aataggaatg gctaatacca ctttaaagcc tgcatttaaa caatgcgaaa
     4861 aatgtggtga ataatggcta tctaaattat taatggcggt tttgcccaat ttagcggctg
     4921 tggtacaaaa gcaactgcct agtttttgac attgcatttg ttgtgataaa gacggtggaa
     4981 taccttcgtc aaccactaaa tacacacggt ctgcattttt atctatgagg cgtactgtgc
     5041 cacctgctgc accaaattca ggcacaatgc gttgtaaaaa attacggcat aattcttcta
     5101 aagactgatg acgattaatg tgttggctaa tatcataaag taccgataaa taacgatttt
     5161 tttgttctaa gctatcggtt ttttcggcaa cacgccgttc taaatcggca tatagttgtt
     5221 gtagttcatc agccatggca ttgaagccca ctgataattg cgaaaattca tcttgtccct
     5281 caataggcaa acgcacagta aagtcgcctg tggtcatttg ttgtaagccc tgatgcaagt
     5341 tagtgacagg ccgaacaatc atcaaatgca gtaaaatgac catcactaag gtgcctgtta
     5401 aggccattac aatgagcatc atttgagagg agcgtagcca aaaggtttgg cgggcatatt
     5461 ctttttcgat gaggttaacc gtgctatgaa ttttgttgac caaggcagcg gtttgctgtt
     5521 gccagtcttg tgctgtgtat gtttcgaata cctttcctgt taatagcaaa tcaagttgag
     5581 gggcaaaatg ctgctgccaa tcttggtcta gctgtattaa atgcgcttga atagccgttt
     5641 ggcgtggcaa aaataacgga cgttgtgggt caccattatg caactcggca agggtttgct
     5701 caaaggcgtg acgtatttgg gtgatgtctt gaatatctgt cgagcttagc gtttgtccta
     5761 cgggggtttt attaacaaca gcggcaattt tccatgcgcg catacgcaaa ctaccactat
     5821 cgttaatggc tgcggcagca ccctcgagtt gccatgatag ccataatgtg tagccaatag
     5881 ccatcaaggc aaacaccaac gctaataaca gcatagtggt tattttggca gtaagccgtg
     5941 tcttaacagt atcaataata ttagccattt taatattaaa ccatatcccc ctgtggttta
     6001 tctcctagtc ataaggctag gtgaatcctg cccttataga tgtctaaaac tcggcttttg
     6061 gccgataacc ttatttttag taagtcttta ctcttatatg tgtaaaagga ctttttgcat
     6121 tgcatcacgg gtgtggtggg tatgttctcc tttgtttttt ctggcacgcg aaggagggca
     6181 tacacatatc ctcaaaataa tacaggatag ctgctcatgg cttctaaaga ttttcaaaaa
     6241 tactccgtct taattgcaag cacactagca tttactgtgt gttttatgac atggatgatg
     6301 tttggtgtga taggtattcc tatcaaaaaa a
//
LOCUS       DAOQMB010000096         6295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5268_length_6295_cov_4.715865, whole genome shotgun sequence.
ACCESSION   DAOQMB010000096 DAOQMB010000000
VERSION     DAOQMB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6295
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5268_length_6295_cov_4.715865"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5042)
                     /locus_tag="PLY05_04920"
     CDS             complement(<1..5042)
                     /locus_tag="PLY05_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like protein"
                     /protein_id="HNC02679.1"
                     /translation="MKNKIFSQSATVLLTAALLQACGGGGGGDSAPTATNTAPTNVSL
                     VEASSAGSTVVTASWLPATDDSTTAANLTYEIHASTDTNFVPSASTLKLKGANLASAR
                     IEGLLPSTQYTLKLVVLDKDGLKTVSNSLQVTTSSTPSEQMAGVTVKTMTAGQTLAMT
                     DTSLTLAAGAPVPKVGEFVASAEGDGYLRKVTAVNIVGGQTVLTTEQAALNEVLQKAE
                     LSSSFKIKSVPVEVAETPTQAGLMRVSDGTQSDQANFAWQNTGFKLNTTTYQTNTNMP
                     SPVLMQAGLASASANPSFTESSKKIDFDDGFGTFSKVTVPESISIAERATGEFKAVLK
                     ILNDDKEGLPWDRKDVPLTLCESELVKVVNEKGESVAGLVKLGALESVQSQSATDSVR
                     VAHQIIKVDANFEKASEKPYVAVFKARVDEAKDGCKSPSKETFEYNVDIYVTTANDFP
                     QQQTSPLSFGKEFKVDNDITFTIDPVIESEIRLDNNLGPDSLSYARLELKTAPQVHQV
                     LKISASASAKLDETKTLFTRKFTKVYLAGQVPVVMVGEYGVDVRVDGDVTGAMDATET
                     IDLGFSELSYGLVYQNGQYNTISKRVPVYSFTVDGNASAKANLKVSLVPRLTVKFYGI
                     ATGKAYVEPYGLAEAGIKGHIKAGVSDAGDFGDADYQLTEAQLSVGLDAYLYADLSIF
                     KKTLVSFPEGKNQNTPYRELYPLKMLPNTKIMALPTLSGELVATTPRTENSRSLLIRA
                     KADNLPNPLTFLPRGNKPWIEFSNWTQPKVVFDAPNTLDTNGYKLVASDTTAGDFWFT
                     YTQTGKYILRLSGYSSLGVWARQYTEVVVELTDTDEDGMIDQWEQTFGVTDPAADDDN
                     DGVTNLQEFLAGTNPKLADVEQAKVDTDKDGMPDAWEWRYGLNPYSDADATQDPDGDK
                     ITNVNEYKAGTNPKLADANALLKVSNANPTVNTAVSFSLATVFDSVKEIVWKFGESVV
                     NVMENLADAISKMFNTVGDVLVSATLKDSTGAEITTVSTTVNVKPLVCPENQVEQGGK
                     CVDKPSTVVFEDNFNDTTNGVTVGNWTAIVEGTNPSVQEANQRLEVTLPANSVDSAKQ
                     VFEAGYQNTCQLDGDFDVQVDYALLDFPVYNGVRVGLTVGDRVRQSSTLYTVERMSLS
                     LQEGGLAASDIVAMHSGGSVDATGDTGGSLRLVRAGSTLNGYRYVNNAWQLVGSESIS
                     TNALPVTISAWSHNDRFDNKNVKVAFDNFKVTKGTLVGGSCKAGGDNRYNIEWHYPNL
                     GTLITGREDKEVSTAVTASDGVEVVGFMNKSKFDVDIIGNRSVKLFNFRSYSNYPNIT
                     QFTSNAFNGFVLRSVNGDFSSATFALDINGDGDFADEGDIAATSSRIVIADDYVAANV
                     AGLSYSPSTIIYIEFQKPTWTTNPANGHQYAAVNCGKWTQCEAQAVALGAHLVSVNDA
                     AENAWLVSTFTPDKNYWIGLTDKDQEGVWKWTSGEVFGYSNWVTGEPNNGYGGTANES
                     YVHMNFNYPSRTNVIGKWNDTQNDITGTGIGGIPYNSSAMGIFEKSSTSSYSIEWHYP
                     NIGTLITGRQNNEVKVTVTASDDVEVVGFMNAPKFDVDIIGNRVKLFNFRSYSAYPNM
                     TQFTANTFNGFVLRSVNGAFSNATVAIDNNGDGDYLDAGELAPTSSKITIGADYIAAN
                     LSEANLSPSTVM"
     gene            complement(5060..5821)
                     /locus_tag="PLY05_04925"
     CDS             complement(5060..5821)
                     /locus_tag="PLY05_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02680.1"
                     /translation="MSAIIPNLAHSQPLVQISVATGIMQQDYKELSANTPNGIFNQET
                     GNLNIWQIQANYLPTLSISKLNLPLSLQGLYRHSNGDTQYDGYLQQGQMLIPFESTTK
                     NNLQDIQVSIGLRLTPQPVQVVPFVAYVAHTWQRGLSQYHETFSHKSLALGLSSLWQI
                     NHKWEILGLFSVGQMQSAKINVPQVSFSASLGTQTIKTIQTQLAYSLNPHWQIALSAN
                     YQRYGYGQSILSNGFFEPESITKQRSFLTSLNYVY"
BASE COUNT         1686 a         1446 c         1274 g         1889 t
ORIGIN      
        1 tacatcactg tactaggact taaattagcc tccgacagat ttgccgctat ataatctgct
       61 ccaatagtta ttttggaaga tgtaggcgca agctctcctg catccaaata gtcaccatcg
      121 ccattgttat ctatcgcaac ggttgcattg ctaaacgccc catttacaga acgtaaaaca
      181 aagccattga aggtattagc ggtaaactgc gtcatgtttg gataggctga ataagacctg
      241 aaattgaaca acttcacgcg gttgccgata atatctacat caaatttagg tgcattcata
      301 aacccaacga cttcaacatc atcactcgct gttaccgtta ctttcacctc attattttgt
      361 cggccagtaa ttaatgtgcc tatattggga taatgccatt caatgctata acttgatgta
      421 gatgattttt caaaaatacc cattgctgaa ctattataag gaataccacc tatgccagtt
      481 ccagtaatat cattctgggt atcattccat ttaccaatca cattagtacg cgatggataa
      541 ttaaaattca tatggacata gctttcatta gcagttccac catatccatt attaggctca
      601 ccagttaccc aattgctata cccaaacacc tcaccactcg tccatttcca taccccttct
      661 tggtctttgt cggttaaacc aatccagtaa tttttatcag gtgtaaaagt tgacaccaac
      721 cacgcattct ccgccgcatc atttacactt actaaatgcg cccctaatgc cactgcttgc
      781 gcttcgcatt gtgtccattt gccacagtta acggcggcgt attggtggcc gttggcgggg
      841 ttggttgtcc aggtgggctt ctgaaactca atataaataa ttgtactcgg tgagtagcta
      901 agaccagcta cattggctgc cacatagtca tcagcaatga ctatccgcga ggatgttgcc
      961 gcaatatcgc cctcatctgc aaaatcgcca tcaccgttaa tatccagtgc aaacgttgca
     1021 ctactaaaat cgccatttac agaccttaaa acaaaaccat tgaaggcatt agaagtaaac
     1081 tgcgtaatgt tcggatagtt cgaataagac ctaaaattga acaatttcac acttctatta
     1141 ccgataatat ctacatcgaa cttggattta ttcataaacc caactacttc tacaccatca
     1201 ctggctgtta ctgctgttga aacctcctta tcttctcgac cagtaatcaa tgtacccaaa
     1261 tttggataat gccattcgat gttatacctg ttatccccac ccgccttaca actacccccc
     1321 accaaggtgc ctttagttac tttaaagtta tcaaaagcca ctttgacatt tttattgtca
     1381 aaacggtcat tatgactcca tgcagaaatg gtgacgggta aggcattggt agaaatggat
     1441 tcgctgccaa cgagctgcca tgcgttattt acataccgat agccatttaa ggtggaacct
     1501 gctctcacta gccgcaaact tccgcctgtg tcgcctgttg catcaacgga acccccactg
     1561 tgcattgcta cgatatcgct ggcagccaat ccaccttctt gcaagctcaa actcatacgc
     1621 tcaaccgtat aaagcgtaga ggactggcgt actctatcac ccaccgtcaa cccaactctc
     1681 accccgttat acacagggaa atccagcaag gcataatcca cttgcacatc aaaatcacca
     1741 tccaactgac aagtgttttg atagcctgct tcaaaaacct gcttcgcact atcgacagaa
     1801 ttggcaggta atgtcacttc caaacgctga ttggcttctt gcacacttgg gtttgtacct
     1861 tcaacaatcg ctgtccagtt gcctactgtt acgccgttgg tggtgtcgtt aaaattatct
     1921 tcaaaaacaa ccgtgcttgg cttatcaaca cacttacccc cttgctctac ctgattttcg
     1981 gggcatacaa gaggtttaac attaaccgtt gtcgaaacgg ttgtaatctc cgcacctgta
     2041 ctgtctttta aggttgctga taccaaaaca tcgcccacgg tgttaaacat tttgctaatg
     2101 gcatctgcca agttttccat cacattaacc accgactcgc caaacttcca aacaatttct
     2161 tttaccgagt caaagactgt cgccaagcta aacgacaccg ccgtattaac tgtaggattg
     2221 gcattagaca ctttaagtaa ggcattggca tcggctaatt taggattagt ccctgctttg
     2281 tactcgttaa cattggtgat tttgtcaccg tcggggtctt gggtagcatc agcatcgctg
     2341 taaggattca aaccgtaacg ccattcccac gcatcaggca taccgtcttt atcggtatca
     2401 accttagctt gttcaacatc agccaatttg ggatttgtgc ctgccaaaaa ctcttgtagg
     2461 tttgttacac cgtcattgtc atcatcagca gcagggtctg ttacaccaaa ggtttgttcc
     2521 cattggtcaa tcatgccgtc ttcatcggta tcggttaact caaccaccac ttcggtatat
     2581 tgacgcgccc atacgcccaa agagctatag cctgatagac gtaaaatgta tttacctgtt
     2641 tgtgtatagg taaaccaaaa gtcacctgcg gtagtatcag aagcgactaa tttataaccg
     2701 ttagtatcga gcgtatttgg cgcatcaaac accactttag gctgtgtcca gttgctaaac
     2761 tcaatccacg gtttgttacc tctagggagg aaagttaaag gattaggtaa attatccgcc
     2821 ttggcacgaa tcaacaggct acggctgttt tcggtacgtg gtgttgtagc aactaactca
     2881 ccactcaatg ttggcaatgc cataattttt gtattgggca gcattttcaa aggatacaac
     2941 tcgcggtaag gcgtgttttg atttttgcct tctgggaaag atactagggt ctttttaaat
     3001 atcgacaaat cagcatatag gtaggcatct aaacccacac tgagctgcgc ttcggttaat
     3061 tgataatcgg catcaccaaa atcccccgca tcagacacac ctgctttaat atgccctttg
     3121 atacctgctt cggctaaacc ataaggctct acataggctt tgcctgtcgc aattccataa
     3181 aacttgacgg ttaaacgtgg tactaaagat acttttaggt ttgcttttgc cgaggcattg
     3241 ccatcaacgg taaagctata cacaggcaca cgtttactaa tggtattgta ttgaccattt
     3301 tggtaaacca agccataaga taattcacta aaacctaaat cgatggtttc tgtggcatcc
     3361 attgcgcctg ttacgtcacc atcaacgcgc acatctacgc catattcacc caccatcacg
     3421 acaggcactt gccctgctaa ataaactttg gtgaatttgc gagtaaataa ggtttttgtt
     3481 tcatctaact ttgcactagc actggcacta attttgagaa cttgatgcac ttgtggcgcg
     3541 gtttttaact ccaagcgcgc ataagacaaa ctatctggcc cgagattgtt gtctaagcgt
     3601 atttcacttt caatcacagg gtcaatggta aaagtaatgt cgttatccac cttaaactct
     3661 ttgccaaaac tgagtggact ggtttgttgt tgcggaaaat cgttggctgt tgtgacataa
     3721 atatcaacat tatattcaaa ggtttcttta gaggggcttt tacaaccgtc tttagcttca
     3781 tcaacacgcg ctttaaaaac agcaacatac ggtttttcag acgctttttc aaaattggca
     3841 tcgaccttga taatttgatg agccacacgc acactatcag tcgctgattg gctttgcacg
     3901 gactccaatg cccctaactt aaccaagcca gccacagact cgcccttctc gtttacgact
     3961 tttaccaatt cactttcgca taatgtgagt ggtacgtctt ttctgtccca agggagtccc
     4021 tctttatcat cattcaatat ttttaacact gctttgaact cgccagtcgc tctttcggca
     4081 atagatatgc tttcgggtac agtgacttta gagaaagtac caaaaccatc atcaaagtcg
     4141 atttttttac tactctcagt aaatgacgga tttgcagaag cacttgccaa acctgcctgc
     4201 atcaaaacag ggcttggcat gttggtattg gtttgatagg tagtggtatt gagtttaaag
     4261 cctgtatttt gccaagcaaa attggcttgg tcagactgcg taccatcact cacacgcatc
     4321 aaacctgctt gtgtaggggt ttccgcaact tcaacaggga cagatttaat tttaaaagag
     4381 ctagataact cggctttttg cagcacttca tttaacgccg cttgctcagt cgtcaaaact
     4441 gtttggccgc caacgatatt aacggctgtt actttacgca aatagccatc accctcagca
     4501 ctggctacaa attcacccac tttggggact ggcgcacctg ccgctaaggt caacgaggta
     4561 tctgtcatgg ctaaggtctg tccagccgtc atcgttttga cagtgacccc tgccatttgt
     4621 tcacttggcg ttgacgaggt tgtgacctgt aagctattac ttacagtttt taggccgtct
     4681 ttatctagca ccaccagctt gagcgtgtat tgtgtacttg gcaataagcc ttcaatacgt
     4741 gcggatgcca aatttgcacc cttcaattta agcgtacttg ctgacggtac aaaattggtg
     4801 tcagtgctag cgtgaatttc ataagtcaaa ttggcggctg ttgtactgtc gtcggttgca
     4861 ggtaaccaac ttgcggtgac gacggtagag ccagccgaag aggcctcaac gagcgagaca
     4921 tttgtaggcg cggtatttgt tgctgtgggc gcactatcgc cgccgccccc accaccacat
     4981 gcctgtagta aagcagccgt taataacacg gtggctgact gactaaaaat tttatttttc
     5041 atattaaaat ccgttgtttc taataaacgt aattcaaaga agttaaaaag ctacgttgtt
     5101 tggttattga ttcaggctca aaaaagccat tacttaaaat tgattgccca tagccgtaac
     5161 gctgatagtt ggcggacagc gcaatttgcc agtgcggatt gaggctatag gctaactgtg
     5221 tttgtatagt tttaatggtt tgtgtgccta aggaggctga aaatgacact tggggaacat
     5281 ttatctttgc ggactgcatt tgtcccacac tgaatagccc aaggatttcc catttatgat
     5341 tgatttgcca caaagaagac aggccaagtg caaggctttt atgactaaag gtttcgtggt
     5401 actgggatag gccacgctgc catgtatgag ctacataggc cacaaagggg acaacttgga
     5461 caggctgagg cgtaaggcgt aagccaatgc taacctgtat gtcttgtagg ttattctttg
     5521 ttgtactttc aaaggggatg agcatctgcc cttgctgtaa gtagccgtca tactgcgtat
     5581 cgccgttact atggcgatat aggccttgca aagacagtgg caagtttagc ttgctgatag
     5641 acagagtggg taaatagttt gcttgaattt gccagatatt gagattgcct gtttcttggt
     5701 taaagatgcc gtttggcgta ttggcggaga gttctttata gtcttgttgc atgatccctg
     5761 tggcaacact gatctgcact agcggttgac tgtgcgccaa gttaggaata atagcggaca
     5821 atgttaatag gcttttatag cagccttggc ttgagggtat caccgctaaa catccttatt
     5881 accaaggtgg agttttgtaa ttatttgtag tgatattttt atcaaataat ctgttttttg
     5941 tacagtgact aacctgctga attattcttt cttatggggt tttattacaa tttttttaat
     6001 ttttagtcct aactatgtaa aagaatcatt tattaaggcc atgtttgtga tttaagggcg
     6061 attttaggtc agaggtttag tggaacgtaa agccgagtta agcggttaat atagagagcc
     6121 catcacgctc taacagccct aaaaacaaaa aagcccctcc ttgccgaagg gctgttttgg
     6181 tggtgatttt ttgttttgtt ttgtttgggg tgttttctag gtgtatttaa cataatgctg
     6241 attatgcgag gagttttctg gtcgattttt gagctgttat gagtggcttt aaagg
//
LOCUS       DAOQMB010000097         6288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5282_length_6288_cov_15.257180, whole genome shotgun sequence.
ACCESSION   DAOQMB010000097 DAOQMB010000000
VERSION     DAOQMB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5282_length_6288_cov_15.257180"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1759)
                     /gene="ilvA"
                     /locus_tag="PLY05_04930"
     CDS             complement(245..1759)
                     /gene="ilvA"
                     /locus_tag="PLY05_04930"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HNC02681.1"
                     /translation="MIRLEDYVRRTLQAKVYDIAKETPLDEMRYLSRRLKNKILLKRE
                     DLQPVFSFKIRGAYNKIASLSQEDRAKGVICASAGNHAQGVALSASKLGIKGIIVMPQ
                     TTPDIKVNAVKSFGGHVVLHGDAFDEAAAYAKQLAADKGYSFIHPYDDLEVIAGQGTV
                     AMEILRQQTQGIDAVFIPVGGGGLMAGMAAYIKYVRPDIKVIAVEPEDAACLKVAMDA
                     QERVVLSQVGLFADGVAVAQIGEQTFKVAQHCVDEVITCNTDEICAAIKDIFEDTRSI
                     AEPAGALSLAGLKKYCQREQCEQQTLIAIDSGANMNFDRLRHISERTEIGEKREAIFA
                     VTIPEKAGSFKTFCQMLGKRNLTEFNYRYADNKEAYVFVGIQLRAGTQERLELKENLE
                     KKGYAVLDLSDDEMAKLHIRHLVGGHAGLADEHLFRFEFPERPGALLNFLSRLSGHNI
                     SLFHYRNHGAAYGRVLIGLQLGKKSKEDLLASLQAVGYPFSEESSNPAYQLFLR"
     gene            1864..2559
                     /gene="rpiA"
                     /locus_tag="PLY05_04935"
     CDS             1864..2559
                     /gene="rpiA"
                     /locus_tag="PLY05_04935"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005177689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HNC02682.1"
                     /translation="MGKAYFCGEPSMTQDQLKRAVAEAALAYVEKDEILGVGTGSTAN
                     FFIDALASMKQHIPAAVASSKATAERLQKIGIEVVDLNSVDSLSIYVDGADEVNSHLH
                     MIKGGGGALTREKIVAAVAKKFICIADASKKVDVLGAFPLPVEVIPMARSYVARELVK
                     LGGSPVYRQGFVTDNGNVILDTYNLSIVNPVELEATLNQIVGVVTNGLFAKRGADVLL
                     LGAADGVKTFLKS"
     gene            2556..3308
                     /locus_tag="PLY05_04940"
     CDS             2556..3308
                     /locus_tag="PLY05_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02683.1"
                     /translation="MKLKTLILLFSATVSGTLSASVLTDLRPCSKVGAFNFEQKTPYQ
                     ALKTTVPIVGNCLVVPNKPQEKVCMQGLYQAEKDNDADYFKMSYWREGKQLVEWKSDG
                     FLMAPAETFNVELLNVDGDSDLELVVSVMTSEGMGMGVQSSDIYVLDRKDLTISAPIS
                     VEDYGRISGFYGKSGQACRLLVSTWQSHEDDKGNGGLYAEGAWYLLKDTQWLEAKAWP
                     KMSRRYLFRFQDERAAAKAPLLWFLDKTTEME"
     gene            complement(3321..3782)
                     /locus_tag="PLY05_04945"
     CDS             complement(3321..3782)
                     /locus_tag="PLY05_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNC02684.1"
                     /translation="MNPSVSFQSLRSKALAMLTRREHSAYELRQKLLEQGASESEILL
                     ILTEFKEKNWQNDERFCQAFIRHHAQKGHGEVVIKQELKQRGIVNSDFITTELEEYDW
                     FLLAQETRSKKFGLELPTERKEQARQYRFLQYRGFNNEQCWHALKYNHEMD"
     gene            complement(3792..4631)
                     /locus_tag="PLY05_04950"
     CDS             complement(3792..4631)
                     /locus_tag="PLY05_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HNC02685.1"
                     /translation="MNELFSELLEPSLLTLVYGCLAICAGSAIWSLIVKDWHWLKSAK
                     YAVYGLIVLLPCYLWQITVELTDTDWPNFFSSAFLVISQSNLGQMWLLMCGGQLLALF
                     AILYRPLTAPYQQITLLAAVFVYTLARAASGHAAEAGLLGGAVWIHAIHIWAACLWLG
                     LIICYLLTFNHNTNNLPYNTQQLSELATLCLMALVLTGFADSLRIYQLADNFWQSEYS
                     KILFLKLAIIAVALGLAATNRFYTLPRLANKASLFYWLVSIESIVIGLALGVASYLGG
                     LMP"
     gene            complement(4628..>4834)
                     /locus_tag="PLY05_04955"
     CDS             complement(4628..>4834)
                     /locus_tag="PLY05_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HNC02686.1"
                     /translation="ERLEPAFCTLNVFSEDGKKLVLDKPQINDKTLVVPLPDNLAKGR
                     YTVKWSVVSVDGHRTKSSYQFVLK"
     assembly_gap    4835..4934
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4935..>6288)
                     /locus_tag="PLY05_04960"
     CDS             complement(<4935..>6288)
                     /locus_tag="PLY05_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC02687.1"
                     /translation="LVEMGTSLAILFGSMLGGFLIAIKPDGKELVSFTVLAIAVLGFI
                     TSWRIPKAESSNPNLKINWNPISETWRNFQFIKQNRTILLSILAISWFWFYGATFLTQ
                     IPNYTKTVLGGDEHVVTFLLTVFSVGIAIGSLLCERLSDKKVELGLVPFGSIGLTCFG
                     VDLYFATHGLQPETTQTFSQFIANSTHQRVIFDLLGIGIFGGFYIVPLYALIQARSEP
                     SHRARIIAGNNIVNAFLMVLSAALAIVVLSVFHRSISELFLVTAILSAVVSLYIYTVI
                     PEFFMRFLVWIFLSFFYKIKKTNLQHIPEEGACVLVCNHVSFYDALILSAAIRRPPRF
                     VMYHTIFKTPILSFIFRTAKAIPIAPAKEDPALLEKAYADIAQALAEGEVVCIFPEGR
                     ITDTGEMYPFKNGIMRIIEQTPVPVVPIALRGMWGSISSRKDGAAFSKLPRRFRSPVE
                     V"
BASE COUNT         1959 a         1250 c         1260 g         1719 t
ORIGIN      
        1 cctcgttaag atagagattg gaggcgatgc tcaactgcgt tgttaacgcg gcgcgaatta
       61 tatagctaat tttgacaaaa gtgtgaaata ttttaagtta ttttgaaaag ttaaatacaa
      121 ggataagggc tgttgatgtt ttgattgtga ttgcctacat gcaaaacgtc aacagtttct
      181 aatatctgac agatactcta atcaatattg gtaactgtca gatgaggtca gacgctaata
      241 caacttagcg caaaaacaac tgataagcag gattgctact ttcttcgcta aacgggtagc
      301 caacggcttg caatgaggct aacaagtctt cttttgattt tttacctaac tgtaagccaa
      361 tcaaaacgcg accatacgct gcaccatgat tgcggtaatg gaacaagcta atattatggc
      421 cacttaaacg cgataaaaag ttcagcagag cacctggccg ttcagggaat tcaaacctaa
      481 aaagatgttc gtcggctaaa cccgcatgac cacctactaa atgacgaata tgcagttttg
      541 ccatttcatc atccgataaa tctaaaacgg catagccttt tttctcaaga ttttctttca
      601 gctctaagcg ttcttgtgtg cctgcacgta actgaatgcc gacaaaaaca taagcctctt
      661 tattatcggc ataacgatag ttaaattcag tcagattacg tttacctagc atctgacaaa
      721 aggttttaaa tgaacctgct ttttcaggaa tagtcacagc aaaaatggct tcgcgttttt
      781 caccaatttc agtacgttca gaaatatggc gcaaacgatc aaagttcata tttgcacctg
      841 aatcaatggc aatgagggtt tgctgctcgc attgttcacg ttgacaatat ttttttaatc
      901 ccgctaaaga taatgcgcct gctggctcgg caatcgagcg tgtatcttca aaaatgtctt
      961 taatggcagc acaaatttcg tcagtattac aagtaatcac ttcatctaca cagtgttggg
     1021 caactttaaa ggtttgttca ccaatttgtg ccacagcaac accatcagca aataagccca
     1081 cttgtgacaa taccacgcgc tcttgcgcat ccatggcgac ttttaaacaa gcagcatctt
     1141 caggctctac ggcaatcact ttaatgtcag ggcgtacata tttaatataa gcagccatcc
     1201 ctgccattaa gccaccacca cccacaggaa taaacacggc atctatgcct tgggtttgtt
     1261 ggcgcaaaat ctccatcgcc acagtgcctt ggccagcaat cacttctaaa tcatcataag
     1321 ggtgaataaa actataacct ttatcggcag ctaattgttt ggcataagct gcggcttcat
     1381 caaacgcatc accatgcaac accacatggc caccaaagct ttttactgca ttgactttaa
     1441 tatcaggtgt ggtttgtggc ataacaataa tgcctttaat acccaatttg cttgctgaca
     1501 aagcaacacc ttgagcatga ttgccagccg aggcacaaat tacgcctttg gcacgatctt
     1561 cttgggaaag ggaagcaatt ttgttataag cgccacgaat tttgaacgaa aaaacgggtt
     1621 gcaagtcttc gcgtttgagt aaaatcttgt tttttaaacg ccgtgataaa tagcgcattt
     1681 catccaaagg cgtttctttg gcaatatcat acacctttgc ttgtagtgta cggcggacat
     1741 aatcttctaa gcgaatcatc ttaattagtt atcttaaaat aaaacagcca tattaagcag
     1801 ttatcgtcta cttctcaagg tataatatca gcgacttttt taagacctgc gcatttcgca
     1861 gaaatgggta aggcatattt ttgcggagaa ccttcaatga cccaagatca attaaaacgc
     1921 gccgttgctg aggctgcttt agcttatgtt gaaaaagatg aaattttggg cgtaggcaca
     1981 ggctcaacag ccaacttttt tattgatgcg ttggctagca tgaaacaaca cattccagca
     2041 gcggttgcca gttcaaaagc aacggctgag cgtttgcaaa aaattggcat tgaagtcgtc
     2101 gatttaaact ctgtggacag tttaagcatt tatgttgatg gggcagatga agtaaacagt
     2161 catttgcata tgattaaagg tggcggtggg gcattaaccc gcgagaaaat tgtggcagca
     2221 gtggccaaaa aatttatttg tattgctgat gccagtaaaa aagtagatgt gttaggggca
     2281 tttcctttac ctgtggaagt cattcccatg gcacgttctt atgtggcacg agagttagtt
     2341 aaattgggtg gtagccctgt ttatcgtcaa ggttttgtca ccgataatgg taatgtcatt
     2401 ttagatactt ataatttaag tattgttaac cctgttgaat tagaagctac cctcaatcaa
     2461 atcgttggtg tggtcacaaa tggcttattt gctaagcgtg gtgctgatgt gttgttgtta
     2521 ggtgcagcag atggtgtaaa aactttttta aaatcatgaa actaaaaacg cttattttat
     2581 tattcagtgc cacagtgagt ggcactttat cggcttcagt gctgactgat ttacgaccct
     2641 gctcaaaagt aggggcgttt aattttgagc aaaaaacgcc ttatcaagcc ctaaaaacca
     2701 ctgttcccat tgttggcaat tgcctcgttg ttcccaacaa acctcaagaa aaggtttgta
     2761 tgcaagggct ttatcaagca gaaaaagaca atgatgctga ttattttaaa atgagctatt
     2821 ggcgagaggg caaacagctt gttgaatgga aaagtgacgg ttttttaatg gcaccagccg
     2881 aaacatttaa tgttgaatta ttaaatgtgg atggcgactc tgaccttgag ttagtcgttt
     2941 cggtcatgac cagtgaaggc atgggcatgg gcgtacaaag tagtgatatt tatgtactgg
     3001 atcgaaagga tttgacgatt agtgcgccga tctctgtaga ggattatggc cgaatttcag
     3061 ggttttatgg taagagtggt caagcctgcc gcttattagt gagcacatgg caaagccatg
     3121 aagacgataa aggtaatggt ggtttgtatg ctgaaggcgc gtggtatctg ttaaaagata
     3181 cgcaatggtt agaagctaaa gcatggccca aaatgagtcg ccgttatttg tttagatttc
     3241 aggatgagcg agcagccgct aaagcaccat tgttatggtt tttagacaaa acgacagaga
     3301 tggagtaagg gcaacagcct ctaatccatc tcatgattat attttaaggc atgccaacat
     3361 tgttcattat taaagccgcg atattgcaga aaacggtatt ggcgggcttg ttctttgcgt
     3421 tcagtgggta gctctaggcc aaattttttg ctgcgagttt cttgtgccag taaaaaccaa
     3481 tcatattctt ctaattcagt cgtaataaag tcgctattaa caataccacg ttgtttgagt
     3541 tcttgtttga ttaccacttc accatgacct ttttgagcat ggtgacgaat aaaagcttgg
     3601 caaaaacgct catcgttttg ccagtttttc tctttaaatt ctgtcaaaat tagcaagatt
     3661 tcactttcgc tcgctccttg ttctaagagc ttttgacgca attcataggc gctatgctct
     3721 cgacgcgtta acatggcaag ggctttacta cgtaggcttt gaaagctgac tgacggattc
     3781 atggtcgttt cctagggcat taagccaccc aaataactcg ccacacccaa agctaagcct
     3841 ataacaatag actcaatact gacgagccaa taaaaaagcg atgctttatt tgccaaacgt
     3901 ggcaaagtat aaaagcgatt agtcgcggct aagcctaagg caacagcaat aatagcgagt
     3961 tttagaaata aaattttaga atattccgat tgccaaaagt tatcggctaa ttgatagatt
     4021 cttaaactat cggcaaaacc tgttaacact aaggccatta aacagagtgt tgctaattct
     4081 gagagttgtt gggtgttata cggtaagttg tttgtgttgt gattaaatgt taataaataa
     4141 caaataatta agccaagcca taaacacgca gcccaaatat gaatggcatg aatccacaca
     4201 gccccaccta ataaacccgc ttcggcggca tgaccactgg cagcacgtgc aagcgtataa
     4261 acaaaaacgg cggctaagag ggtaatttgt tggtagggtg ctgttagcgg acgatataaa
     4321 atagcgaaca acgcaagcaa ttgtccaccg cacattaaca gccacatttg ccctaaattg
     4381 gattggctga taactaaaaa agcagagcta aaaaagtttg gccagtcggt atcggttagc
     4441 tcaacggtga tttgccataa ataacaaggc aataacacta ttaagccata aacagcatat
     4501 tttgcgctct tgagccaatg ccagtcttta acaatcagtg accaaatggc agaaccagca
     4561 cagattgcta aacaaccata aactaaagtt aatagacttg gctctagcaa ctccgagaac
     4621 aattcattca tttaagcaca aactgatagc tgcttttggt gcgatgtcca tcaaccgaaa
     4681 ccaccgacca tttaacggta taacggcctt tggctaaatt atcaggcaaa ggcacaacta
     4741 gcgttttatc attaatttga ggtttgtcta aaactagttt tttgccgtct tcactaaaga
     4801 cattgagtgt acaaaaagct ggctctaggc gttcnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnacttca acaggtgagc gaaagcgacg ggggagttta ctaaatgctg
     4981 caccatcttt gcgactacta atgctgcccc acataccacg taaagcaatc ggtacgacag
     5041 gcacaggcgt ttgttcaata attctcataa taccattttt aaaagggtac atttcgcccg
     5101 tatcggtaat ccgtccttca gggaaaatac aaaccacttc accctcagct aaagcttggg
     5161 caatatctgc atacgctttt tctaataagg cagggtcttc tttagcaggg gcaataggaa
     5221 tcgctttggc cgttctaaaa ataaagctaa gaattggcgt tttaaaaatg gtgtgataca
     5281 taacaaaacg tggtggacgg cgaatcgcag ccgataaaat taaggcatca taaaagctga
     5341 catgattaca gactaaaaca caagcacctt cttcgggaat atgctgcaaa ttggtttttt
     5401 tgattttgta aaagaaactc aagaaaatcc acaccaaaaa gcgcataaaa aattcgggaa
     5461 tcaccgtata aatataaagt gaaacaactg cacttaaaat ggcagtgact aaaaataact
     5521 cggaaatgct acgatgaaac acggataaaa cgacaattgc caacgccgca gataacacca
     5581 ttaaaaaagc attaacaata ttgttgcctg caataatccg tgcccgatga ctcggctctg
     5641 agcgtgcttg aattaaggca tacaatggaa caatataaaa accaccaaaa ataccaatgc
     5701 ctaataaatc aaaaatcact cgttgatgag tgctattggc aataaactga ctaaatgttt
     5761 gagtggtttc aggttgtaag ccgtgtgtgg caaagtataa atctacgcca aaacaggtta
     5821 aaccaataga gccaaatggc actaaaccca gctccacttt tttgtcagat aaacgttcac
     5881 acaataatga accaatggca atacccaccg aaaacaccgt taacaagaag gtgacaacgt
     5941 gttcatcccc acctaaaaca gttttggtgt aattgggaat ttgcgttaaa aacgttgcgc
     6001 cataaaacca aaaccacgaa atagccaaaa tagataataa aatggtacga ttttgtttaa
     6061 taaattgaaa attacgccat gtttcactaa tcggattcca gttgattttt aaatttgggt
     6121 tactcgactc tgctttagga atacgccaac tggtgataaa acctaaaaca gcaatggcca
     6181 acacagtgaa tgacactaat tctttaccat caggcttaat ggcaattaaa aagccaccta
     6241 acattgagcc aaacagaatg gctaaagaag tccccatttc gactaagg
//
LOCUS       DAOQMB010000098         6281 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5298_length_6281_cov_15.002730, whole genome shotgun sequence.
ACCESSION   DAOQMB010000098 DAOQMB010000000
VERSION     DAOQMB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5298_length_6281_cov_15.002730"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="PLY05_04965"
     CDS             complement(<1..1313)
                     /locus_tag="PLY05_04965"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC02688.1"
                     /translation="MVTSSARYNNIAVLLHWLIAAAIIFQLGLGWRMGDEPKGAGAYA
                     LFQLHKSIGFSILFLSLFRVVWRLTHKAPELPNTMPQWEQWAAKLAHLAFYGIMLGLP
                     LTGWLLVSASSTNIPTVLFGVVPFPHLPFIAELATDAKHQLHELAEDAHGLLAVATLG
                     LLLLHIGAVLKHQLIVKDSVFSHMAVGAKAGWGEWRLWLILASVPVVFMSAWWYPTAK
                     PAAPVAQQAEILTEELTPIEEEATPVVDSPAIVRPKDEAVKTAEQPQTVVEQKVENTE
                     PVVWQVNAKKSRLGFMTSWSGEQVLGEFSDWQANIVFAENALEKSHIEVKVNLAKVST
                     GDSQRDEALPTADWFNAEQYPQAIFKSNKIISKGTQRYQAQGTLSLKNVTQKVNLDFV
                     LNVKDKLAKAQGTAQLDRTVFGVGQGEWAATDSIPAIVKVQFKLEA"
     assembly_gap    1360..1459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1461..2060)
                     /locus_tag="PLY05_04970"
     CDS             complement(1461..2060)
                     /locus_tag="PLY05_04970"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HNC02689.1"
                     /translation="MSKLLTHLMLTASLMTGAVAYAAPDANPANMPAGSYVLEKTHAS
                     INAKVMHHGYAFYHFRFDKFDASYDYDPKNPTASKVVVTVDTKSMNTGYAKADKEFPV
                     EFLAADKHPTATFKSNKITITQGNKGTMAGDLTLGGVTKPVTLDVVFNGAGKDMWGAM
                     RSGFSATTTIKRSEFGLTKYVPAIGDEVALAIEVEFVKK"
     gene            complement(2152..3306)
                     /locus_tag="PLY05_04975"
     CDS             complement(2152..3306)
                     /locus_tag="PLY05_04975"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HNC02690.1"
                     /translation="MRILVLGLLTSLLAACVTPQKPTTPPKPETVTAVYKAQDWQALP
                     NWPGEQLTRSWPAWLNSCKRLANRPVWKDICAEAVMLGSQDDASIKAFFERHFSPWQI
                     TTNAGSDTGLVTGYYEPLLKGSLNPKAGSAPFYSVPDNLLNIDLVKQYPDLKGKRLRG
                     RIDGVRTVIPYWTRQEIAEGKIANNAKVLAWADDAVEAFFMEVQGSGRIQLEDGTIIR
                     LGYADQNGHPYKSIGKWLIDKGEMTADQASMQSIQAWAKANPQRLQEMLNANPSVVFF
                     KYLSGNDGPIGALNVPLTDEASGAVDPKFVPLGSPIYLSTTRPNDSQPMTRLIHAQDT
                     GGAIAGPIRVDYFWGFGSQAGQLAGKMKQKGQVWLLWPKNLPLPEQAVNK"
     gene            complement(3385..4284)
                     /locus_tag="PLY05_04980"
     CDS             complement(3385..4284)
                     /locus_tag="PLY05_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC02691.1"
                     /translation="MPVVPLRDGQNLYVRTIGRGQPVLMLAGLGMHSGHWLPFIVPFL
                     HRYKFYLPDFRGVGRSSHLKFNQADVFHNHYQDTADVIKHFNLKDYLLVGISLGGTTA
                     LHLQREQGFADVKAYLHIDQSPCIGNQEDWAFGLFGSQQAQIFSRMSVLHELLSQYQD
                     YRQLADLPKNVRKQAAKTLADILVLMSNQPKLRKVFEYLLISPKILAKHLPLTNVADC
                     CAYLHAYSHLAHDYRDSLQQCATPMTVIVGMNSPLYHPQGQMQIVDYAQQAKVVKFHK
                     SGHVPLTDEPLKFICELKRFLQH"
     gene            4600..5985
                     /gene="hemN"
                     /locus_tag="PLY05_04985"
     CDS             4600..5985
                     /gene="hemN"
                     /locus_tag="PLY05_04985"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HNC02692.1"
                     /translation="MMPTAIEFDLELIQRYDLSGPRYTSYPTAVQFHNHFSEQAYLQA
                     IADSNQSRRPLSLYFHLPFCAHVCYYCACNKIITANRKRAAPYLEHLHQEIALQGQLF
                     DKNRVVSQLHWGGGTPTFISDEQMSALMDCTRQHFNLLDDDTGDFSIEIDPREVTPAK
                     IGHLRKLGFNRMSFGVQDFDPLVQQAVNRVQSFGQTYEAVMAARDNGYHSINVDLIYG
                     LPHQSVASFSHTLDQVLELSPDRLSVFNYAHLPDLFKVQKQIDSQQLPSAATKLAIFQ
                     LIIERLSQAGYVYIGMDHFAKPDNELAIAQQQGTLYRNFQGYSTHADCDLVGLGITSI
                     GVVADTYSQNLKTLEAYGEHIEQGRVPVFRGVALTADDKLRRAVITQLICHFELEFAT
                     IEQQYQINFADYFAYELQQLPRMVNDGLISLDHHKLVVLPRGRLLIRNICMVFDFYLR
                     QSQEKKFSKVI"
     gene            complement(6048..>6281)
                     /locus_tag="PLY05_04990"
     CDS             complement(6048..>6281)
                     /locus_tag="PLY05_04990"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC02693.1"
                     /translation="AYRLLMMVNNSAASSPIARRVLTISQDQLAHFLAVSRQTTNQLL
                     KSLEQQSVIKLHRNSIEVLDIQALEALSEGVVS"
BASE COUNT         1813 a         1338 c         1170 g         1860 t
ORIGIN      
        1 gcttctaatt taaactgcac tttaactatg gctggaatac tatcagttgc cgcccattca
       61 ccttgaccaa caccaaaaac tgtacggtct aattgtgccg tgccctgagc tttagccagc
      121 ttatctttaa cgttcaaaac aaaatctaaa ttcacttttt gagtgacatt ttttaggctc
      181 aacgtgccct gtgcttgata acgttgcgtg cctttactga tgattttatt cgatttaaaa
      241 atggcttgcg gatactgctc ggcattaaac cagtcagccg ttggtaaagc ttcatcacgt
      301 tggctatcac ctgtactcac ttttgccaga ttaactttta cttcaatgtg cgacttttct
      361 agcgcgtttt cggcaaagac aatatttgct tgccaatcac taaactcacc taaaacttgc
      421 tcacccgacc aagaggtcat aaaacctaaa cgtgattttt ttgcgttaac ttgccaaacc
      481 acaggttcag tattttcgac tttttgctca acgacagttt gcggttgttc ggcggttttt
      541 actgcctcat ctttaggtct aacgattgct gggctgtcaa ctactggcgt agcttcttcc
      601 tctataggcg ttaattcttc ggttaaaatt tctgcttgtt gagcaacagg tgcagcaggt
      661 tttgctgttg gataccacca tgccgacata aacaccacag gcacactggc taaaatcaac
      721 cataaacgcc attcgcccca acctgctttt gcaccaacgg ccatgtggct aaataccgaa
      781 tctttaacaa ttaattgatg ttttaatacc gcgccaatat gcaacagcaa taaacccaat
      841 gttgcaactg ctaataaacc atgagcatct tcagccagtt catgcaattg atgttttgca
      901 tcagtggcta gttcagcaat aaaaggtaaa tgtggaaacg gtacaacacc aaataacacc
      961 gttggaatgt tagtagagct agcagacact aataaccaac ctgttaaagg caaacccaac
     1021 ataatcccat aaaacgctaa atgagcgagc ttggcagccc actgctccca ttgtggcatc
     1081 gtattgggta attcgggagc tttatgggtt aaccgccaaa caacacgaaa taagcttaaa
     1141 aataaaatgc taaaaccaat cgacttatgt aattgaaata aggcataagc tcctgcgcct
     1201 ttaggttcat cacccatacg ccaacctaaa ccgagttgaa aaataattgc tgcggcaatt
     1261 aaccaatgta gcaacacggc aatattgtta taacgtgctg aggatgtaac catttttgct
     1321 ctcctaaaaa aatagggcgt tatatcgccc tatttttaan nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnc ttattttttc acaaattcca cttcaatcgc caaagcaact
     1501 tcatcgccaa tagcaggcac atacttggtt aagccaaact cagaacgttt aatggtagtc
     1561 gttgctgaaa aaccactacg cattgctccc cacatatctt taccagcacc attaaatacc
     1621 acatctaaag tgacaggctt agtcacgcca cccaacgtta aatcaccagc cattgtgcct
     1681 ttattgcctt gagtaatggt aattttattg gatttaaagg tagccgttgg atgtttgtca
     1741 gccgctaaaa actctacagg aaactcttta tctgctttag cataacctgt attcatcgat
     1801 tttgtatcaa ccgtgaccac cactttagac gctgttggat ttttaggatc ataatcataa
     1861 gacgcatcaa atttatcaaa gcgaaaatga taaaaggcat aaccatgatg cattactttt
     1921 gcattaatac tggcatgggt tttttctaag acataactac ctgctggcat attggcagga
     1981 ttagcatcag gtgcagcata agcgactgca cctgtcatta aagaagcggt taacattaaa
     2041 tgagtcaaca atttagacat ttttcttttc cttcaaaaga tttttgcgta gtgccaaagt
     2101 tacgcaatct taatcttttt gtctatgaag gctttggaaa cgtattgttg cttatttatt
     2161 aacagcttgt tcaggcagtg gtaaattttt tggccacaat aaccaaactt gacctttttg
     2221 tttcattttg cctgctaatt gtcctgcctg agaaccaaag ccccaaaaat aatcaacacg
     2281 aattggccct gcaatcgcgc cgcctgtgtc ttgagcatga atcaagcgtg tcatcggctg
     2341 actgtcattt gggcgtgtag ttgataaata aatcgggcta cctaaaggca caaacttagg
     2401 atcaaccgca ccactcgctt catcggttaa aggaacgttt aatgcgccaa ttggcccatc
     2461 attacccgat aaatacttaa agaaaaccac actaggattg gcattaagca tctcttgtaa
     2521 gcgttgcgga ttggcttttg cccatgcttg aatgctttgc atcgacgctt ggtcggcggt
     2581 catttcgcct ttatcaatta accatttgcc aatcgactta taaggatggc cattttgatc
     2641 ggcatatccc aaacgaataa tagtgccatc ttcaagctga atgcgcccag agccttgcac
     2701 ttccataaaa aaagcttcga ctgcatcatc cgcccatgct aatactttag cattattggc
     2761 aattttgcct tcggcaattt cttggcgtgt ccaataagga atcactgtcc gcacaccatc
     2821 aatacgacca cgtaaacgtt tgccttttaa atcggggtat tgtttaacta ggtcaatatt
     2881 taataagtta tcaggtacac tataaaatgg cgcactgccc gctttgggat ttaaactgcc
     2941 tttgagcaag ggttcataat atcccgtcac taaacctgta tctgaacctg cattggtagt
     3001 aatttgccat gggctaaaat gacgctcaaa aaatgctttg attgacgcat catcctgact
     3061 acccaacatc accgcttcgg cacaaatatc tttccaaaca ggccgatttg ctaaacgttt
     3121 acacgaattt aaccatgcag gccaactgcg ggtgagttgc tcacccggcc aattgggtaa
     3181 agcttgccaa tcttgggctt tataaacggc agtcaccgtt tcgggtttag ggggagtagt
     3241 tggtttttga ggtgtcacac aagcggctaa taaactggtt aataagccta aaactagaat
     3301 acgcatgagc ttttccatga agacgcaaaa tgcggccatt atcctgcaaa atcacgctaa
     3361 ataagagcct tataatgtgt atttttagtg ttgcaaaaag cgttttagtt cacaaataaa
     3421 cttaagtggc tcatcggtta acggcacatg gccagattta tgaaacttaa caactttcgc
     3481 ttgttgggca taatcgacaa tctgcatttg cccttgtgga tgatacaacg gtgaattcat
     3541 gccaacgata acggtcatgg gggtggcaca ttgttgcaag ctgtctcgat aatcatgcgc
     3601 taaatgacta taggcgtgaa gatatgcaca acaatctgcg acattggtca gcggtaaatg
     3661 tttggctaat atttttggac taattaataa atactcaaag actttacgca gttttggttg
     3721 attgctcatt aataccaaaa tatcggctaa ggtcttggct gcttgtttac gaacgttttt
     3781 aggcaaatcg gctaattgtc gataatcttg atattgcgac aataactcat gcaacacact
     3841 catacgacta aaaatttgtg cttgttgact gccaaacaaa ccaaatgccc aatcttcctg
     3901 attgccaata caaggagact ggtcgatatg taaataggct ttgacatccg caaaaccttg
     3961 ctcacgctgt aaatgtaatg ctgtcgttcc gcctaacgaa atgcccacta ataaatagtc
     4021 ttttaaatta aagtgtttaa tcacatccgc cgtatcttga taatgattat gaaacacatc
     4081 ggcctgatta aacttgagat gacttgaacg gcctacccct cgaaaatcgg gcaagtagaa
     4141 tttatagcgg tgtaaaaaag gcacaataaa cggcaaccaa tggccactat gcatgcccaa
     4201 gcctgccaac attaacacag gttgaccacg accaattgta cggacatata ggttttgacc
     4261 atcgcgtaat ggaacaacag gcatttttta ttctcttaaa attgtcgtta taccttaaga
     4321 aataatcagc acactgacaa tggtaatttt gacacaataa agaggagctt gagccatata
     4381 actcaagctc tcgacgctta atttaagcag tattaaacac aacggtttgg ctaaaacgtt
     4441 gtgcttgacg aacactaaat cattcgacat tgttttattt cctcattttt acactctttt
     4501 ttagctaaaa aactaaacta caggttaata accacttttt ttatatggtt ttattgatag
     4561 actatgttac tgtcgatttg cttagaagag agaataacga tgatgcccac tgctatcgaa
     4621 tttgatttag agttaattca acgctacgat ttatcaggcc ctcgttatac ctcgtacccc
     4681 actgccgtac aatttcataa tcattttagc gagcaagctt atttacaagc catcgcggac
     4741 tccaatcaaa gtcgtcgccc cttatcttta tattttcatc tgccgttttg tgctcatgtt
     4801 tgttattact gtgcgtgtaa taaaatcatt accgctaatc ggaaacgtgc tgcaccatac
     4861 ctagagcatc ttcatcaaga aattgccctt caaggacagt tatttgataa aaatcgtgtg
     4921 gttagccaac tacattgggg tggtggcacg cctactttta tcagtgacga acaaatgtcg
     4981 gcactcatgg actgcacacg ccaacacttt aatttattag acgatgatac tggcgatttt
     5041 tcgatagaaa ttgacccgcg cgaagtcact cccgcaaaaa taggtcattt acgcaaactt
     5101 ggttttaatc gcatgagttt tggcgtgcaa gactttgatc ctttggtgca acaagcagtg
     5161 aatcgggtac agtcctttgg ccaaacctat gaagcggtga tggctgcccg tgataatggt
     5221 tatcactcga ttaatgttga tttaatttat ggtctgcccc accaaagcgt tgccagtttt
     5281 agccacacct tagatcaagt cttagaatta tcacctgaca gattatcggt atttaactac
     5341 gcgcatttgc ctgatttatt taaggtacaa aaacaaattg atagccaaca attgcccagt
     5401 gcggcaacaa aattggcgat ttttcagtta attatagagc gattaagcca agcaggttat
     5461 gtgtatattg gcatggatca ctttgctaaa cctgataatg aactggccat tgctcaacaa
     5521 caaggtacgt tgtatcgcaa ctttcaaggt tatagcactc atgcagactg tgatttggtt
     5581 ggtttaggca ttacttcgat tggtgtggtt gctgatactt atagtcaaaa cctaaaaacc
     5641 ttagaagctt atggtgaaca cattgaacaa ggtcgcgtgc ctgtgtttag aggcgtcgca
     5701 ttaacggctg atgacaaatt gcgtcgtgcg gttattactc aattaatttg tcattttgaa
     5761 ctagagtttg ccaccattga gcagcaatat cagattaatt ttgctgacta ttttgcttat
     5821 gagctacaac aattgccacg tatggttaat gatggtttaa tcagtttaga tcatcataaa
     5881 ttagtggtgt tgccgcgtgg acgattgtta attagaaaca tttgtatggt ctttgatttt
     5941 tatttacgtc aaagtcagga aaagaaattt tctaaggtga tttaatttag gttcagttca
     6001 acgaccaaaa ctccgcaccc tgagcggagt tttaggcagt caactcttta gctcaccacc
     6061 ccttctgaca aggcttctaa agcttgaata tccaacactt ctatactatt acgatgtaac
     6121 ttaattaccg attgttgttc taaacttttt aataattgat tggtcgtttg tcggctcact
     6181 gctaaaaaat gcgctaattg atcttgcgaa atggttaaga ctcggcgcgc aatgggtgaa
     6241 cttgccgcac tgttattcac catcattaac aaacgataag c
//
LOCUS       DAOQMB010000099         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5324_length_6268_cov_16.848865, whole genome shotgun sequence.
ACCESSION   DAOQMB010000099 DAOQMB010000000
VERSION     DAOQMB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5324_length_6268_cov_16.848865"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLY05_04995"
     CDS             complement(<1..434)
                     /locus_tag="PLY05_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNC02694.1"
                     /translation="MHAQGFIQDLAIIMLVAGFVTLLFHRLKQPVVLGYLLAGVIIGP
                     HTPPFALIHDEHTIHVLAELGVVFLLFSLGLEFSIKKLAQVGMPALIAGVTEIIVMIW
                     LGYYIGAFFGWSTMDSLFLGAMLAISSTTIIIKALEGLNLKK"
     gene            complement(452..667)
                     /locus_tag="PLY05_05000"
     CDS             complement(452..667)
                     /locus_tag="PLY05_05000"
                     /inference="COORDINATES: protein motif:HMM:NF003316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HNC02695.1"
                     /translation="MTELLAERVTELECKQAFQEDLLETLNKIVVEQQQHIDILQEQI
                     KIIYQQLKSLQPSNIADLADEVPPPHY"
     gene            756..2663
                     /locus_tag="PLY05_05005"
     CDS             756..2663
                     /locus_tag="PLY05_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005403628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC02696.1"
                     /translation="MSLLSFRAVSLSFGGPLLLKAADFSLEQGERVCILGRNGEGKSS
                     LFKLLTGENQPDSGEITRQQGLVVASLSQEVPSDLTGDVFDIVAAGLGEAANLLQRYH
                     HLSHACELGDNQACMELANLQSDMDSQDCWSLQHKVEQILNRMELDGNASLESLSGGR
                     KRRVMLARALVQEPNILLLDEPTNHLDVSSIQWLENFLASYTGTVLFISHDRAFIERI
                     ATRIVELDRGILRSFNGGYQQYLVEKPQLLEAEAKQNAVFDKKLAEEEVWIRQGIKAR
                     RTRNEGRVRALIELRRERVARRERVGNAQVQIQEAEKSGKLVFDIKNLTVKAGDKTLV
                     KDFTAMVMRGDKIGLLGENGVGKTSLIRVLLGENPPSAGTVHLGTKIEVAYFDQLRNQ
                     LDEEKTVLDNIAHGSEFLEINGERKHALSYLQDFLFSAQRARTPVKALSGGERNRVLL
                     ARLFSRPSNVLVMDEPTNDLDIETLELLEERLAAYQGTLLLISHDRAFLDNVVTDTWA
                     FMGDAVVEEFVGGYQDWLRQTENRRVKTNNKTTNTMEKTESAKPVLQNKEKPTNIKRK
                     LSYNEQRELEALPSKIAALEQEQQLLQDKLNDSNFYVNSAQEAVLASQRLATIDEELL
                     TLLERWTELES"
     gene            2859..3593
                     /locus_tag="PLY05_05010"
     CDS             2859..3593
                     /locus_tag="PLY05_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02697.1"
                     /translation="MKRGWLGSALVASIMLTGCAHQNPAAQMQAFYHAPSNAYSLSLS
                     SPAFIGQVNMTEQCEPYGGSVNLWDSKNRFFRVDYLNFIEHPTVRSPAFANDRTLNEL
                     VMNYYEEVVLPKERNILSRKILFRDTVNSTMGEGMLTILSLEMNKKALPPDVLDNTVY
                     YGFFVFRRGDFSYIVQHRQEIYQPERMKAMLVTLAESMTTPGKNFFTQQVNDLGVVDK
                     VFGSQAEIDAWKKAMQCDNKKSKKSS"
     gene            3610..4257
                     /locus_tag="PLY05_05015"
     CDS             3610..4257
                     /locus_tag="PLY05_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02698.1"
                     /translation="MNKVTTRCLFVIAAITLAQYSFAAKQPSKKTIFIKELQNLITPY
                     NTQIIIDRQRIHKIAEQYLHDDEISEPDFNWLKKTAESYQLTPKQRNDQAFFNALLKR
                     VDVIPNSLIISLAIVNGAWVHQNPSKRFDFLCVTPCPTNSDAEQRLHHFFLSVNTHRT
                     YHHFREQRYQLRNQQQTITGRQLADSLLSSPAYLSQRHVIKKLVSTRQWQKMDTL"
     gene            4254..5132
                     /locus_tag="PLY05_05020"
     CDS             4254..5132
                     /locus_tag="PLY05_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC02699.1"
                     /translation="MKAQTLTISDKIALNVRIWGEENNLAPTIVMVHGFPDNSLIWQK
                     VAERLAPLFKIIAYDVRGAGESSIPSSTQDYKIKYLVEDLAAVINAFSPTQPIHLVGH
                     DWGSIQCWEAVTTPLLQAKIASFTSISGPSLDHAAYWIRQGLSKGSLDSKVKIINQLA
                     HSWYIAAFHLPLASQVTWRVAFNRWPARFARLTKLHGEDFASQARDGAHGVKLYRANF
                     IERLFKPQERKTNIPVQLIIPTQDKFVTAGLFDNLSEWANRVWRHEIDAEHWVQRSHP
                     DEVSAYIREFVDFIDS"
     gene            complement(5180..5596)
                     /locus_tag="PLY05_05025"
     CDS             complement(5180..5596)
                     /locus_tag="PLY05_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02700.1"
                     /translation="MTENLNSEVFSLASQLYVRMRRCSGRVVDAIYMAQNEQYAQEIL
                     KIAAQEKDQEILEIVQRFQTLLAKTEPKIVESTPEIPVLTETIKEPTVEKTDIFSMTL
                     RNLRRKAEVVENSPTAQPHIEELEEQVAHHYTGALR"
     gene            complement(5679..>6268)
                     /locus_tag="PLY05_05030"
     CDS             complement(5679..>6268)
                     /locus_tag="PLY05_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNC02701.1"
                     /translation="LIGASLLWVGWFGFNAGSALAADGRAGMAMLVTQVAAATGTLAW
                     MLLEFLRRGKASLLGACSGAVAGLVAITPASGFVDVKGALLIGLIAGVGCYWGATGLK
                     RMLGADDSLDVFGIHGIGGVIGAILTGVFAKHHLSGLETTVTIQAIGAFVTLAYSFII
                     SLILLFLLDLIIGLRVDAENESIGLDLTQHGEQVP"
BASE COUNT         1965 a         1239 c         1168 g         1896 t
ORIGIN      
        1 tcttttttta aatttaatcc ttctaaggct ttaataataa ttgtggttga agaaatagct
       61 aacattgccc ccaaaaatag cgaatccatc gtactccaac caaaaaatgc tcctatgtaa
      121 taccccaacc aaatcatcac gataatttct gtaacacccg caattaaagc tggcatacca
      181 acttgagcca attttttaat actaaactct aaacctaaag aaaataataa aaacacaacg
      241 cctaattcgg ccaacacatg aatggtatgt tcgtcatgga ttaacgcaaa tggaggtgta
      301 tgagggccaa taattacgcc tgccagtaaa taccccaaaa caacaggttg ttttaaacga
      361 tgaaatagta atgtgacaaa tcctgctacc aacataataa ttgcaagatc ttgtataaat
      421 ccttgggcat gcattaattt atttactcct attaataatg aggcggtggc acttcatctg
      481 ctaaatcagc aatatttgat ggctgtaatg atttaagttg ttgataaatt atttttattt
      541 gctcttgtag aatatcaata tgttgctgct gttcaacgac aattttgttt aatgtttcta
      601 ataaatcctc ttgaaacgct tgtttacatt ctagctctgt aactcgttca gctaataact
      661 ctgtcatatt ctttcctcaa atgcgtgttt atttagcaat catgtcaaat tattgctacc
      721 attatctctt tcttagataa accttagcaa acattatgtc tttattatct tttcgtgctg
      781 tatctttatc ctttggtggt cctttgttgc tgaaagcagc tgattttagt ttagaacaag
      841 gtgaacgcgt gtgtattttg ggacgtaatg gtgagggtaa atctagttta tttaagttat
      901 taacaggtga aaatcagcct gatagtggcg aaattacccg ccaacaaggt ttagtggttg
      961 cctcgttatc tcaagaagtt ccttctgact taacaggtga tgtatttgat attgtagcag
     1021 cgggtttggg cgaagcagca aatttactac aacgttacca ccatttaagc catgcctgtg
     1081 aattaggtga taatcaagct tgcatggaat tagcaaactt acaaagtgac atggatagtc
     1141 aagactgttg gtcattacag cataaagtag aacaaattct taatcggatg gaattagatg
     1201 gcaatgcttc gcttgaatca ttgtcggggg ggcgtaaacg ccgtgttatg ttagcgcgtg
     1261 ccttagtaca agaacccaat attttattat tagatgaacc cacgaaccat ttagacgtta
     1321 gcagtattca gtggctagaa aattttttgg ctagctatac aggtacggta ttatttatta
     1381 gccatgatcg agcttttatt gagcggattg cgactcgtat tgttgaatta gatcgtggca
     1441 ttttgagaag ctttaacggc ggctatcaac aatacttagt cgaaaaacca caacttttag
     1501 aagcagaagc taaacagaat gctgtttttg ataaaaaact tgccgaggaa gaagtgtgga
     1561 ttcgccaagg catcaaagca cgtcgtactc gtaatgaagg tcgagtacgc gccttaattg
     1621 aactacgccg tgaacgtgtt gcccgccgtg agcgtgtggg taatgcccaa gtacaaattc
     1681 aagaagccga aaaatcgggt aaattagttt ttgatattaa aaacctcacc gtaaaagcag
     1741 gtgataaaac cttagttaaa gattttacgg cgatggttat gcgtggcgac aaaattggtt
     1801 tattgggtga aaatggcgtt ggtaaaacca gtttaattcg ggtgttatta ggtgaaaacc
     1861 ctccgagcgc aggtacagtt catttaggca caaaaatcga agtggcttat tttgaccaat
     1921 tacgcaatca attagacgaa gaaaaaaccg ttttagataa tatcgctcat ggctctgaat
     1981 ttttagaaat taatggcgaa cgtaaacacg cgttaagtta tttacaagac ttcttatttt
     2041 cggctcaacg cgctcgcaca cctgtaaaag ccttatcggg tggtgaacgt aatcgtgtat
     2101 tgttagctcg tttatttagt cggccatcta atgtattggt gatggatgaa ccaaccaatg
     2161 atttagacat tgaaacatta gaattactgg aagaacgtct tgctgcttat cagggaacgt
     2221 tattattaat tagccatgat cgtgcctttt tggataatgt cgtcactgat acatgggcgt
     2281 ttatgggcga tgcggtggta gaagaatttg ttggtggtta tcaagattgg ttaagacaaa
     2341 cagaaaatcg tcgtgttaaa accaacaaca aaacaacaaa tactatggaa aaaactgaat
     2401 ctgccaagcc tgtcttacaa aacaaagaaa aaccgacaaa cataaaacgt aaactaagtt
     2461 acaatgagca acgtgaatta gaagccctac cctcaaaaat tgcggcttta gaacaagaac
     2521 aacagttatt acaagacaag ctcaacgata gtaactttta tgtcaactca gctcaagaag
     2581 ccgtgttggc aagtcagcgt ttagcgacca ttgatgagga attattaacg ttgctcgagc
     2641 gttggacaga attagagtcc taaacttgat ttaatgacag tcaaattgca acttttctat
     2701 gtagttcatg tatttttagc aacataaact cgctaatagc ggttaaaatg accgccaata
     2761 tagaactgaa ggattgcaca ctaaatgctt tttgcacctt agtggacata atagaaaaac
     2821 agcaatccta attatgtagg tattgtgagg agaatagaat gaaacgcgga tggcttggct
     2881 cagcattagt tgccagtatc atgttaacgg gttgcgccca tcaaaaccct gctgcacaaa
     2941 tgcaagcttt ttatcatgcc ccttctaatg cttatagttt aagcctaagt agcccagctt
     3001 ttattggtca agtaaatatg accgagcaat gtgagcctta tggtggcagt gtaaacttat
     3061 gggatagtaa aaatcgcttc ttccgcgtcg attatttaaa ctttatcgaa catcctactg
     3121 tacgctcccc tgcatttgcc aacgatcgta cactgaatga attagtgatg aactattatg
     3181 aagaagttgt actgcccaaa gagcgtaata ttttaagtcg taaaatcttg ttccgcgaca
     3241 cggttaacag tacgatgggc gaaggtatgt tgacgattct tagcttagaa atgaacaaaa
     3301 aagccttacc tcctgatgtt ttagataata cggtatatta tggtttcttt gttttccgtc
     3361 gtggtgactt ttcatatatc gttcaacatc gtcaagaaat ttatcagcca gaacgtatga
     3421 aagcgatgtt ggttacactt gccgaaagta tgaccacacc aggcaaaaac ttttttactc
     3481 aacaagttaa cgatttaggt gtcgttgata aagtattcgg ctctcaagca gaaattgatg
     3541 catggaaaaa agccatgcaa tgtgacaata agaagagtaa aaaatcttct tagttaaatg
     3601 gcggcaataa tgaataaagt aaccactcgc tgtttatttg ttattgccgc aataacactt
     3661 gcccaatata gctttgctgc caaacaacca tcaaaaaaaa caatttttat caaagagtta
     3721 caaaacctta ttacccccta taatacacaa atcattatcg accgtcagcg cattcacaaa
     3781 attgccgaac aatatttaca tgatgatgaa atcagtgaac ccgattttaa ttggctaaaa
     3841 aaaaccgcag aatcatatca actcacccct aaacaacgta atgatcaagc tttttttaat
     3901 gctttattaa aacgagtcga tgtgattccc aatagtctaa ttatcagtct tgctattgtt
     3961 aatggcgcat gggtacatca aaatccctct aaacgttttg attttttatg tgtaactcct
     4021 tgccccacca atagcgatgc cgaacaacgc ctacaccact tcttcctaag cgttaataca
     4081 caccgtactt atcatcattt tcgtgagcaa cgttaccaac tacgtaatca acaacagaca
     4141 attacgggca gacaattagc cgatagcttg ttatcatcgc ccgcctatct ttcacaacgt
     4201 catgtgatta aaaaattagt ctcaactcgg caatggcaaa aaatggatac tttatgaaag
     4261 cccaaacctt aacaatttct gacaaaattg cacttaacgt tcgtatttgg ggtgaagaaa
     4321 ataatttagc acctacgatt gttatggtgc atggctttcc agacaatagc cttatttggc
     4381 aaaaagtggc tgaacgttta gctccgttat ttaaaattat tgcttatgat gtgcgtggtg
     4441 caggtgaatc gagtattccc tcctccactc aagactataa aatcaaatat ttagttgaag
     4501 atttggcagc cgtgattaac gcctttagtc caactcagcc aattcattta gttggccatg
     4561 attgggggtc aattcaatgc tgggaggccg taaccactcc tcttttgcaa gctaaaatag
     4621 cctcttttac ctcaatttct ggcccaagtt tagaccatgc agcgtattgg attcgtcaag
     4681 gtttaagcaa aggctcttta gacagcaaag ttaaaattat taatcaatta gcacattctt
     4741 ggtatattgc cgcttttcat ttgccgttgg ctagtcaagt cacatggcgg gtggctttta
     4801 accgttggcc tgctcgtttt gctcgcttaa cgaagctaca tggtgaagat tttgcttcac
     4861 aagcgcgtga tggcgcacat ggcgttaagt tatatcgtgc aaactttatt gagcgattat
     4921 tcaaaccaca agaacgtaag accaatattc ctgttcaatt aattattcct actcaggata
     4981 aatttgtcac cgcaggatta tttgataatt tatctgaatg ggcgaatcgt gtatggcggc
     5041 atgaaataga tgccgaacat tgggtgcaac gtagtcatcc agatgaagtc agtgcctata
     5101 ttagagagtt tgttgatttt attgatagtt aaggtatttc ctcttacctc cttgaagagg
     5161 aaatagcggc tctttttatt taacgtaacg cacccgtata atgatgtgct acttgctctt
     5221 ccaactcctc aatatgtggt tgagccgttg gtgaattttc tacaacttca gctttacgac
     5281 gtaaattgcg caatgtcata ctaaaaatat ccgttttttc tactgtaggc tctttaatag
     5341 tctctgttaa cactggaatt tctggtgttg actccactat ttttggctca gtcttggcta
     5401 ataatgtctg aaaacgctga acaatctcca aaatttcctg atctttttcc tgtgctgcaa
     5461 ttttcaaaat ttcttgagcg tattgctcgt tttgagccat atagattgca tcaaccacac
     5521 gtccagaaca acgtctcatc cgtacatata attgtgaagc caaactaaaa acttcagaat
     5581 ttaaattttc tgtcatgatt aatttctcct tatttgagaa ataacattaa tgagagaatt
     5641 aaacatctat gatgatgctt aattctttta aacggatgtt aggggacttg ctcgccatgc
     5701 tgtgttaaat ctaaaccaat agattcattc tcggcatcta cacgtaaccc gataattaaa
     5761 tctaataaaa acaataagat caatgaaata ataaagctat aagccaatgt gacaaatgcc
     5821 cctatggctt gtatggtaac tgtagtttca agccctgata aatgatgttt agcaaaaact
     5881 cccgttaaaa ttgcacctat cactccacca ataccatgaa taccaaatac atctaaggaa
     5941 tcatcggctc ccaacatccg ttttaaaccc gttgctcccc aataacagcc aacacctgca
     6001 attaaaccaa tcaataatgc tcctttgaca tcgacaaaac ctgatgctgg ggtgatagcc
     6061 accaatcctg ctactgcacc tgaacaagca cctaataatg atgctttacc acgacgcaaa
     6121 aattctaaca gcatccatgc taatgttcct gtagcggcag caacttgtgt gactagcata
     6181 gccattcctg cacgtccatc ggctgccaac gctgatcctg cattaaatcc aaaccatcct
     6241 acccacaata atgacgctcc gatcaatg
//
LOCUS       DAOQMB010000100         6254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5350_length_6254_cov_15.638651, whole genome shotgun sequence.
ACCESSION   DAOQMB010000100 DAOQMB010000000
VERSION     DAOQMB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5350_length_6254_cov_15.638651"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="PLY05_05035"
     CDS             <1..728
                     /locus_tag="PLY05_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02702.1"
                     /translation="FRLENESEFLNQLIKYNFSVELIGYTDWLNAVNACQKFWINLLG
                     GSDAQDIARLYIKQRIELIVKTIPSLITMMAWIEYQMWGELPEPVMEVALAKSKNAYS
                     LVEYLWQGEDSLLQTKLLRTHSSVEVWPSSKLFTKALNAFYKKSPQNIQRILDQSNSD
                     QRRVLFWPLIHDYKCAVVNLPVLCGFWSMSPVPMVWWSHHPERQRFIKELLSFDPIWF
                     QVAYNQGCKIALALDAHCEFNRE"
     gene            735..>4159
                     /locus_tag="PLY05_05040"
     CDS             735..>4159
                     /locus_tag="PLY05_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02703.1"
                     /translation="MENLYFSRLLPELAEKSVQAAIRRLHIQNKPLAAYLRNTLSTQL
                     GAKGALLGDPVFEPTFGWQTHSETMQALSGGLLSPQLIDAMDAPAGDTKNEYRFAKEY
                     YPYQHQHDAWSLLSQQPPQSLVVTSGTGSGKTECFLVPVLDDLVRESQCSDKPLVGVR
                     ALMIYPLNALIASQRDRLNAWTTNFGNKIRFCLYNGTTPLSVPQWQRREAMNQVLDRQ
                     SLRKSPPPILVTNATMLEYLLVRHEDAPIIQASQGKLKWIILDEAHSYIGSKAAELAL
                     LLRRVMLAFGVQSEDVHFVATSATLGGQDSEKQLKRFLASVSGLPLERVHVVTGKRYI
                     PKRVSGNKKFVDVSYEELVKVDSHVSGRLYNALCANQIACEIRAQFVSSQGQMPQRLS
                     SLKSVLEEKFDCLFSHQDVLQWIDLLSRAKSKKGESFLPLRLHVFHNVLNGLWACCNS
                     NCSYRKGTPLDNENWPFGRVYFDARNKCLCRSPVYEIRPCSRCHAVYLWANWKEEQSY
                     YLISPEVNTVDDFSADAELVEPDMDMAESHDQSEWSPLLILQPDMLGAKITFFETESF
                     KQCGQEFARTVSLNVSLPSKGWKNEFSCLCCNAVHSPNKPLFRALRLGAPFFQEIILP
                     VLLKYCPDYIGRDEGEVSRPYQGKRLITFTDSRQGTARIALKCQQDSERHWLQSQVYR
                     KLLSEVKQDSADAKQKILYQLENLQQRYATTPEAIKPMIRGLIKTQENELQKLEQPQA
                     VSFKHMVSWLKSQPVLREQILPVYQEKNPEFFDDEEGAVALAKIMLLREFARRPYIQD
                     NLETLGLVSIVYPKLQRIQKAPDFSSHHLLMDVSEWRSFLKIVLDFFIRHHLILRMPN
                     EWSSWSGNRIPCKVLITPNVVPKYSKKNVLWPRANMGMTNSKLVRLLIKALNANVYTE
                     GDRDAINQLFMLAFDELTQVGLLQKQGETWVLDPEDLAFIIPKQTWLCPVTKQILDVT
                     LKEVTPLTPKYPKHNDPLTCLPIAMPSSDSQWQFLAEDERLSRIKEWLKSNHQVASLR
                     NEGHWHDLHDQIMLGATYFHVVEHSAQQTSEQLRKYETGFRQGKINIMSCSTTMEMGV
                     DIGGISVVSMNNVPPHPANYLQRAGRAGRRGETRSVVLTVCKNTPHDQFVFNNP"
     assembly_gap    4160..4259
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4260..5900
                     /locus_tag="PLY05_05045"
     CDS             <4260..5900
                     /locus_tag="PLY05_05045"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNC02704.1"
                     /translation="LSVLSKYQKARYEFNRELPSRDSSTGLVEYAPSAKVVIDGLVYQ
                     SEGITLNWHEPSSKQDVSEIQNIRQVWLCECCGSSINLPTGVVLNECSECGARLSAYG
                     YHNQLTHFNYLDPAGFAVDIFAKPHNDLRTQRFVPPEEPLISAMGQWQALSTPEWGFY
                     RVSEKGRVLHYTSGSHKQGYAVCLSCGRAEEMTDEGKIPVEFSKHHIRLRGSQGRQHA
                     VCEGTENRYSIVPSLRFGHEYQTDVLEFFLLVNKASITDNTISFTLAVAMRNAAAGIL
                     GIEVRELGCEVKWIQDNGQKGYAIVLYDINASGYVSSLEHRIKELFHFTLEALNCPAN
                     CESSCPACLQHFDLRFRTRDLNRQQAIEHIKYAWSENRWWYLGGSFFSPKTLAEHDSL
                     LRAINREFNLADVQELKLFLNGDSSLWQIGQSPLLLWIRKWVAQGHPVKLVFKKDAIS
                     ACSDENKLALHQLAQLKGVSFWQGEVNITMKKSQLCAEVMTARGSIFWAELTANISIP
                     NNSWGNTSDSELWRSLPITATLDLEEIQWPILQRFSKN"
     gene            complement(5877..6134)
                     /locus_tag="PLY05_05050"
     CDS             complement(5877..6134)
                     /locus_tag="PLY05_05050"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HNC02705.1"
                     /translation="MLSTWTKIEPARNCYRFYAISLASDLFNAYVVSCEWGRIGAKKS
                     RRKVEIFNSVDEAMAQIKRIESLRIRHHYQANHDLVLAKSL"
BASE COUNT         1823 a         1142 c         1384 g         1805 t
ORIGIN      
        1 tatttagact ggaaaatgag tccgagtttt taaatcagtt gattaaatat aatttttcag
       61 ttgaattaat cggttatacc gattggctga atgccgttaa cgcatgccag aaattttgga
      121 taaatttgtt aggcggtagt gatgcacaag acattgctcg cctgtacata aaacagcgta
      181 ttgaactgat tgttaaaact atcccatctc tgattaccat gatggcatgg attgaatatc
      241 aaatgtgggg tgagttacca gaacctgtca tggaggtagc attagctaaa tcaaaaaacg
      301 cctatagttt ggttgagtat ttgtggcagg gcgaagactc attacttcaa accaaactgc
      361 ttagaacaca cagttctgtt gaagtatggc caagctcaaa gctatttacc aaagcattga
      421 atgcctttta taaaaaatca ccccaaaata ttcagcgtat tttagatcaa tcgaacagtg
      481 accaacgtcg agtactgttt tggcctttaa tccatgacta taaatgtgct gtggtgaatc
      541 tgcctgtgct gtgtggtttt tggagtatga gtcctgtgcc aatggtgtgg tggtcgcatc
      601 accctgaacg acaacgtttt attaaagagc tgttgagttt tgaccctatt tggtttcaag
      661 tcgcctataa ccaaggctgc aaaatcgcct tggctttgga tgctcattgt gaatttaaca
      721 gggagtaact caggatggag aacctctatt tttcacgact gttgcccgag ttggcagaaa
      781 agtctgtgca ggcggcgatt cgtcgtttgc acatccaaaa taaaccattg gctgcttacc
      841 tacgaaatac gttgtccaca caattgggcg cgaaaggcgc attattaggt gatcctgttt
      901 ttgagccaac attcggctgg cagactcatt cagaaacgat gcaagcatta tcgggtggcc
      961 tgttatcgcc acagttgatt gatgcgatgg atgctcccgc aggagatact aagaacgaat
     1021 atcgatttgc taaagaatat tatccgtacc aacatcagca tgatgcttgg tcattacttt
     1081 cacaacagcc tccacaatca ttagtggtca ccagtggtac agggtctggt aaaacggagt
     1141 gttttttagt tcctgtatta gatgatttag ttcgagagtc acagtgtagc gataaacctt
     1201 tagtcggtgt tcgagcactg atgatttatc ctctaaacgc cttgattgcc agtcagcgtg
     1261 acagacttaa tgcgtggaca acgaattttg gcaacaaaat tcgcttttgc ctttataacg
     1321 gcacgacacc tttaagtgtt cctcagtggc aacgtcgtga ggcaatgaat caagtgcttg
     1381 accgtcagtc gctaaggaaa tcaccacccc ccattctggt gacgaatgca acaatgttgg
     1441 agtatctact ggttcgtcat gaggatgcgc caatcattca agcctcacaa ggtaagttga
     1501 aatggattat tttggatgaa gctcattctt acattggctc taaagcagcg gaattggcac
     1561 tgttgttacg tcgtgtcatg ctggcttttg gcgttcaatc agaagacgtg cattttgtgg
     1621 caacatcggc aacattaggt gggcaggatt cagaaaagca attaaagcga tttttagcat
     1681 cggtctcagg attaccctta gagcgagtgc atgtcgttac aggtaaaaga tacattccta
     1741 aacgtgtgag tggtaacaaa aaatttgtag atgtcagcta tgaggagctt gttaaagttg
     1801 actcacatgt cagcggacgg ttatacaatg ccctgtgtgc gaaccaaatt gcctgtgaaa
     1861 tccgcgctca gtttgtttca tcacaaggtc aaatgccaca acgtttatct tcattaaagt
     1921 cagtattaga agaaaagttt gattgcctat tttcacatca agacgtttta cagtggatag
     1981 atctgttatc tagagccaaa agcaaaaagg gtgagtcatt tctaccttta cgactgcatg
     2041 tgtttcataa cgttttgaat ggcttatggg catgttgtaa ttctaattgc tcttatcgga
     2101 aaggaacgcc tcttgataat gagaattggc catttggcag agtctatttt gatgcgcgca
     2161 acaaatgcct atgtcgtagt cctgtgtatg aaatacggcc ttgtagccgt tgccatgccg
     2221 tttacctttg ggctaattgg aaagaagagc aaagctacta tttgataagt ccagaggtga
     2281 atactgttga tgattttagt gcagacgctg aactggtaga acctgatatg gatatggcag
     2341 aaagccatga ccaaagtgag tggtcgcctt tgttgatttt gcagcctgac atgttagggg
     2401 caaaaattac gttttttgaa acagaaagtt ttaaacaatg tgggcaagaa tttgccagaa
     2461 cagtatcttt aaatgtcagt ttgccatcta aaggatggaa gaatgagttt agctgtcttt
     2521 gttgcaatgc ggtacattca ccaaacaaac ctttatttag agcattacgc ttgggagcac
     2581 ctttttttca agaaattatt ctgcctgtgt tattaaaata ttgtcctgat tacattggta
     2641 gagatgaagg agaagtatcg cgtccatatc aaggtaaaag attaattact tttaccgata
     2701 gtcgtcaggg gacggcacga attgccctga aatgccaaca agactcagaa cgacactggt
     2761 tacaatctca agtctatcgc aaattattga gcgaggttaa acaagactcc gcagatgcta
     2821 aacaaaaaat attgtatcag ttagaaaatc tgcaacagcg ttatgcaaca acgcccgaag
     2881 caataaaacc aatgattaga ggattgatta agactcaaga aaacgaatta cagaaattag
     2941 agcaaccaca agcagtttcg tttaaacaca tggtttcttg gttgaagtct caaccagtat
     3001 tgcgtgagca aatcctacct gtttatcaag aaaagaatcc agaatttttt gatgacgaag
     3061 agggggcagt ggcattagca aaaatcatgc tgctacgtga atttgcccga cgaccttata
     3121 ttcaggacaa cttagaaact ttgggtttag tgtcaatcgt ctatcccaaa ttacagcgta
     3181 ttcaaaaagc ccctgatttt tcaagtcatc atttgttgat ggatgtatca gagtggcgta
     3241 gcttcttaaa aattgtgcta gattttttta tacgacacca tttgatactg aggatgccta
     3301 atgaatggtc aagttggagt ggtaatcgta ttccttgtaa agtgctgata actcctaatg
     3361 ttgtgccaaa atactctaaa aaaaatgtac tttggcctag agcgaatatg ggcatgacga
     3421 actccaaatt ggttcgttta ttgattaaag cattaaatgc caatgtttac acagagggtg
     3481 atcgtgatgc gattaatcaa ttattcatgt tggcatttga tgagctaaca caggtaggat
     3541 tactgcaaaa gcaaggtgag acttgggtgc ttgacccaga ggacttggcg tttattattc
     3601 ccaaacagac ttggctttgc cctgtgacca aacaaatctt ggatgtcact ttaaaagaag
     3661 tcaccccctt aacaccaaaa tatcccaaac ataatgaccc attgacatgt ctgccgatag
     3721 cgatgccatc atcggattct cagtggcaat ttttagccga ggatgagcgt ttatctcgga
     3781 taaaagaatg gttaaaaagc aatcaccaag ttgcgtcttt acgcaatgag gggcattggc
     3841 atgatttaca cgaccaaatc atgcttggcg caacttactt tcatgtcgtg gaacattctg
     3901 cacagcaaac ctcagagcaa ctacgcaagt acgagacagg ttttcgtcaa ggcaaaatca
     3961 atatcatgag ttgctcaacg acgatggaga tgggggtcga tattggtgga atatcagtcg
     4021 ttagcatgaa taatgtgcca ccacatcctg caaattattt acaacgggca ggacgggcag
     4081 gtcgtcgggg tgagactcgt tcggttgtgt tgactgtctg caaaaatacg cctcatgatc
     4141 agtttgtatt taataatccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     4261 tgagtgtgtt gtctaaatac caaaaggcac gttacgaatt taatcgagag ctaccgagtc
     4321 gtgactcaag tacaggatta gttgaatatg caccaagtgc taaagtggtg attgatggtc
     4381 ttgtttatca gtctgaaggt attaccttaa attggcatga acctagctct aaacaagacg
     4441 tatcagaaat tcaaaatatc cgacaagttt ggttatgtga gtgttgtggc tcgtcaataa
     4501 acttacccac aggtgttgtg cttaatgagt gttcagagtg tggtgctcga ttatctgctt
     4561 atggttatca caatcagttg acgcatttta attatttaga tccagcaggt tttgcagtag
     4621 atatttttgc caagccgcac aatgatttaa ggacacaacg ctttgtgcca ccagaggaac
     4681 ctttgattag tgcgatgggg caatggcaag cattaagtac acctgaatgg ggattttatc
     4741 gggtcagcga aaaggggcgt gtgttgcatt acacatcagg ctcccataag cagggctatg
     4801 ctgtttgcct ttcgtgtggc agagcagaag aaatgacgga tgaaggaaag attcctgttg
     4861 agttttcaaa gcatcatata cgccttagag ggagtcaggg taggcaacat gctgtttgtg
     4921 aaggaactga aaatcgctat tccattgtac ccagtttgcg ctttggtcac gaatatcaaa
     4981 cagatgtttt agagtttttt ctgttagtta ataaagcatc cattaccgat aatacaatat
     5041 cttttacact agctgttgcc atgagaaacg cagcggctgg tattttaggt attgaagtca
     5101 gagagttggg ttgtgaagtc aaatggattc aggataatgg tcaaaaaggc tatgcgattg
     5161 tgctttatga cataaacgca tcaggctatg tttccagttt agagcatcga attaaagaat
     5221 tattccattt caccttagag gctcttaatt gtccagcaaa ttgtgaatcg tcttgtccag
     5281 cttgtttaca acactttgac ctacgattta ggacacgaga cttgaatcga caacaggcaa
     5341 tagagcatat caaatatgcg tggagtgaaa atagatggtg gtatttggga ggaagttttt
     5401 tttcaccaaa gaccttagca gagcatgact ctttattaag ggcaatcaat cgtgaattta
     5461 atttggccga tgtgcaagag ctaaagcttt ttttaaatgg cgattcgtct ctgtggcaga
     5521 ttgggcaatc accattgctg ttatggattc gtaaatgggt agcacaaggt catcctgtta
     5581 aattggtttt taagaaagat gctatatcag cgtgcagtga tgaaaataaa ttagcattac
     5641 atcaattagc tcagttaaaa ggtgtgagtt tttggcaagg tgaggtcaat atcaccatga
     5701 aaaaaagtca gttgtgtgcg gaagtgatga cggctagagg atctattttt tgggcagagt
     5761 taacagcaaa tatttctatt cctaataata gttgggggaa cacgagtgac agtgagttgt
     5821 ggcgtagttt gccaataacg gctacgttgg atttagaaga gattcaatgg cctattttac
     5881 agcgatttag caagaactaa atcatgattg gcttgataat gatgcctgat acgcaatgac
     5941 tcaatacgtt ttatctgagc cattgcctca tcaactgagt taaatatctc taccttacgc
     6001 cgactctttt tagccccaat acgtccccat tcacacgaca ccacataagc gttaaacaaa
     6061 tcactagcca atgaaatcgc ataaaagcgg taacaattac gtgcaggctc aattttagtc
     6121 cacgtagaaa gcatgatatt acccaaggtg ttcaataaag catcaataac cttagagcag
     6181 attctatctc ttttctagct taaataaccc acaaggcgac atacattgtc acttgctggg
     6241 ttatttaggt gggg
//
LOCUS       DAOQMB010000101         6164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5472_length_6164_cov_15.813226, whole genome shotgun sequence.
ACCESSION   DAOQMB010000101 DAOQMB010000000
VERSION     DAOQMB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5472_length_6164_cov_15.813226"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="hemB"
                     /locus_tag="PLY05_05055"
     CDS             complement(<1..842)
                     /gene="hemB"
                     /locus_tag="PLY05_05055"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016802711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC02706.1"
                     /translation="MSQFTFARGTFPQTRMRRLRAQEFSRRLVQETVLTPADLIYPMF
                     VLEGEKRREAIPSMLGIERVSIDELLKEAAELVALGVPAVALFPVTPPEAKSLLAEEA
                     WNADGLAQRATRALKAAFPQLGVITDVALDPFTTHGQDGIIDENGYVLNDVTTEALIK
                     QAISHAQAGADVVAPSDMMDGRIGAIRAALEQAGYPNVCILSYAAKYASNYYGPFRDA
                     VGSAGNLKGGNKKTYQMDPANRMEALHEVGFDLAEGADMVMVKPAMPYLDIIREVKDT
                     YQAPT"
     gene            941..>1584
                     /gene="elbB"
                     /locus_tag="PLY05_05060"
     CDS             941..>1584
                     /gene="elbB"
                     /locus_tag="PLY05_05060"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HNC02707.1"
                     /translation="MKKVAVILSGCGFKDGGEIYEATLTLLALDEADVSYQCFAPDMP
                     QMHVVNHLTGEEMPESRNVLVEAARLARGNVKPITEAKIEDFDALIIPGGFGAAKNLC
                     NFAVKGSDMTVQAEVLAFAQGMNQAGKPVGLVCIAPAMAPKIGGAGTQYTIGNDAGTA
                     AAINAMGGVHVNCAVDECVIDNERKIVTTPAYMYAARIREARAGIQKLVQAVLS"
     assembly_gap    1585..1684
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1685..1927)
                     /locus_tag="PLY05_05065"
     CDS             complement(<1685..1927)
                     /locus_tag="PLY05_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02708.1"
                     /translation="MKLALLTDQEILAIVNPMMDNLMTGSTAINHAQHTQDFTERAKA
                     IVTPEHLAYVCKDYQAKWGFFTHREFVAIFRRPASIA"
     gene            2050..2535
                     /locus_tag="PLY05_05070"
     CDS             2050..2535
                     /locus_tag="PLY05_05070"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC02709.1"
                     /translation="MSHQHTTTITDEEKARILQERLKMGFNMVPFTQAMGLSIKEMSN
                     DKITAEFAMQPQLIGNVFKQVLHGGVIATALDTVGGAMAMSGAYVALKGTAKEERTAR
                     INRLATIDMRIDYLKAGRGELFTATASLLRVGKQICVTRMELHNEKQELIAAGTATYM
                     Y"
     gene            complement(2576..3547)
                     /locus_tag="PLY05_05075"
     CDS             complement(2576..3547)
                     /locus_tag="PLY05_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HNC02710.1"
                     /translation="MRVIPFIFACFYPFFSYAQCPDMEAVIKTPMLTPPIKKHFEHFT
                     NKFNASFFDPWHMVHDEIVQQGQAITVTAKFDYNARFHKDLEQEYVQAYLIGTNINQW
                     KALGRYQTDDEGKVQVNLGTLPIGEYRIRFVVEGDLTSTDGFVSVVEAGRKAVLFDID
                     GTLTKSDFEAYADYVGIKTAKPYPYAIEMVNAYKNKGYQIIFLTARPYWVAKNAREWA
                     TKMGIPLWHYRSNAYGDGPIPPNTQEHKTNYVKYLKNTVGLNIIRAYGNATTDIAAYA
                     DGGLPKAETYIIGTNAGKENTQPIYNDYTYHYSTVVVNTPQSSSCPL"
     gene            complement(3653..5797)
                     /locus_tag="PLY05_05080"
     CDS             complement(3653..5797)
                     /locus_tag="PLY05_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNC02711.1"
                     /translation="MSVKFYNSIEQAITDIINKIDGDIRLGAPLGLGKPNAFINAMYQ
                     RISSMPERCLHIFSALSLTKPTASSDLEARFLEPFVERVFGDYPDLNYVTDLKNNKVP
                     NNISVNEFFLKSGDWLNNANAQQHYINSNYTHIARDMAHNGVNVICQSIAVRDEADGT
                     RRYSLSCNPDLSEDLLDLLQPRREAGERIFVVGVINHKLPFMPNDAEVSADQFDMIIE
                     DAAGTHTLFSTPNMKVGLSDYAIGLHASSLVQDGGTLQIGIGSLGDAIVQGLMLRDRD
                     NVTYQNMIRRLNHNLPLSAELDLTPFKQGLYGCSEMFVNGFLKLIQANIIRRQVYNHT
                     GLQKLLNQGLISETVSLDTLKTLREQDLISSPLTGEDVALLTRFGIFSDDCSVEDGVL
                     IVGEQQISGDLDNAEACQQIASLCLGQRLENGVIMHGGFFLGPADFYQALRDMPDDQL
                     NLINMSTIAFINQLYGDRYGDENLKRAQRVKARFINTCMMATLSGAAVSDALEDGRVV
                     SGVGGQYNFVAQAHELPDARSVLMLRSHRMVDGEVRSSIVKNYGHITIPRHLRDIFVT
                     EYGIADLRGKNDSDIIKALLNISDSRCQEELREWAVSKGKLSADYVIPKAYTNNTPEA
                     LAERLQPFMQHLPSFPFGTDFTKDELNIVKALQSLKTSTEHPLQLLKMVVSSIFKDED
                     DDKVHRYLDRMGFNETHSLKEKVMKRLFMGNI"
     gene            5926..>6164
                     /locus_tag="PLY05_05085"
     CDS             5926..>6164
                     /locus_tag="PLY05_05085"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNC02712.1"
                     /translation="MTNKFDICVIEEEIVCRAGCAACCIAPSISSAIPDMPNGKPADV
                     PCVQLNEQGFCKLFGLPERPKVCSSLKPTFSMCGST"
BASE COUNT         1809 a         1192 c         1300 g         1763 t
ORIGIN      
        1 gtgggtgctt gataggtatc ttttacttca cgaataatat ccaaataggg catggcaggt
       61 ttcaccatga ccatatctgc accttcagcc aaatcaaaac ccacttcgtg taaggcttcc
      121 atacggttag caggatccat ttgataggtc tttttattgc caccttttaa attacccgcc
      181 gaaccgaccg catctctaaa cggcccatag tagttagagg catatttcgc ggcatacgac
      241 aaaatacaga cattcgggta gcctgcttgt tctaaagccg cacgaattgc accaattcga
      301 ccatccatca tatcggaagg tgcgaccaca tctgcacccg cttgcgcatg agaaatcgct
      361 tgtttaatta acgcttcggt ggtgacatca tttagaacat agccgttttc atcaataatg
      421 ccgtcttgac catgtgtggt aaatgggtct aacgcaacat cagtaatcac gcctagttgt
      481 ggaaaagccg cttttaaagc gcgtgttgca cgttgggcta aaccatcggc attccaagcc
      541 tcttcagcta ataaactttt tgcttcgggc ggcgtgacag gaaacaaggc caccgcaggc
      601 acacccaaag ccaccaattc agccgcttct ttgagtaatt catcaattga cacacgctca
      661 atacctaaca ttgacggaat ggcttcgcgg cgtttttcac cctctaacac aaacatcggg
      721 taaattaaat cggcaggtgt taataccgtt tcttgtacta aacgacggct aaattcttga
      781 gcgcgtaaac gacgcatacg ggtttgtgga aaagtaccac gagcaaaagt aaattgggac
      841 ataaggcaac tcaataaaaa taattacatc tgtactctag caaaaattta gagtgctgtt
      901 agatttgcta aataaatttt gtagtgacag gatggaaaaa atgaaaaaag tagcagttat
      961 tttatcaggt tgtggcttta aagatggtgg tgaaatttat gaggccactt taaccttgtt
     1021 ggctttagat gaggcggatg tcagttatca atgttttgcg cccgatatgc cgcaaatgca
     1081 cgtggttaat catttaacgg gcgaagaaat gcccgaaagc cgtaatgtct tggtcgaggc
     1141 ggcgcgtttg gcgcgaggca atgttaaacc aattactgag gctaagattg aagactttga
     1201 tgctttgatt attcccggtg gttttggcgc agcgaaaaac ttatgcaatt ttgctgttaa
     1261 aggcagcgac atgacggtac aagctgaggt attggctttt gctcaaggca tgaatcaagc
     1321 aggtaaacct gtcggtttag tgtgtattgc acctgctatg gctcccaaaa ttggtggagc
     1381 aggcacacaa tacaccattg gcaatgatgc aggtacagcg gctgcgatta acgcgatggg
     1441 tggggtacat gttaattgtg cagttgatga atgtgtgatt gataacgagc gcaaaattgt
     1501 cacaacacca gcgtatatgt atgcggcacg tattcgtgaa gcgcgtgcag ggattcaaaa
     1561 gttagtccaa gcggtgttga gtttnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnggcaat cgacgcaggc cgtctaaaaa tagccacaaa ctcacgatgg gtaaaaaatc
     1741 cccatttggc ctgataatct ttacagacat aagctaaatg ttcgggcgtg acaatcgctt
     1801 tggcgcgttc agtaaaatct tgggtatgtt gtgcgtgatt aatagcggta gagccagtca
     1861 ttaaattatc catcatcggg ttaacgatag ccaaaatttc ttgatcagtt aataaagcga
     1921 gtttcataaa tagaccctaa aaaagtgata agcgcgtaag tcctaaaaag attgtttttt
     1981 tggtcattgc caagcaagca tttgatcggt taaaaccgaa taataaatta taaatagaga
     2041 ctttatgcta tgagccacca acatacaacc acgatcactg atgaagaaaa agcgcgtatt
     2101 ttgcaagagc gtttaaaaat gggctttaat atggtgccat ttacccaagc tatggggcta
     2161 agcatcaaag aaatgtccaa cgataaaatt actgctgagt ttgcgatgca gccacagttg
     2221 attggtaatg tttttaaaca agtgttgcat ggtggagtga ttgccaccgc tttggatacg
     2281 gttggtggtg caatggctat gagtggggca tatgtggcgt taaaaggtac tgccaaagaa
     2341 gaaagaacag cccgtattaa tcgtttagca accattgata tgcgtattga ttatttaaaa
     2401 gcaggtcgag gagagctatt taccgcaaca gccagcttat tgcgtgttgg caaacaaatt
     2461 tgtgtgaccc gtatggagtt acataacgaa aaacaagagt taattgcggc tggtacagca
     2521 acgtatatgt attaaacaat agtctccccc tgacaagggg gagatttaga gggggttata
     2581 acggacaaga actcgactga ggcgtattta ccaccacggt actatagtga taggtgtaat
     2641 cgttataaat gggttgagta ttttctttac ctgcatttgt accaataata taggtttctg
     2701 ctttaggcaa accaccatcc gcataagcag caatatcagt cgttgcatta ccataggcac
     2761 gaataatatt taaacccacg gtatttttta aatatttcac atagttggtt ttgtgttctt
     2821 gggtattagg tggaataggg ccatcaccat aagcattgct acgataatgc cacagtggaa
     2881 tgcccatttt tgttgcccat tcgcgtgcat tttttgccac ccaatacgga cgagctgtta
     2941 aaaaaataat ttgatagcct ttgtttttat aagcatttac catttcaatg gcatagggat
     3001 aaggtttagc tgtttttatc cccacataat cggcataggc ttcaaaatcg cttttggtta
     3061 atgtgccatc aatatcaaat aacacggctt tgcgacctgc ttcaaccaca gaaacaaaac
     3121 catctgttga tgtcaaatcg ccttcaacca caaagcgaat acggtattcg ccaataggta
     3181 atgtgcctaa attaacttga actttacctt catcgtctgt ttggtaacgt cctaaagctt
     3241 tccattgatt gatgtttgtg ccaattaaat aggcttgaac atattcttgt tctaaatctt
     3301 tgtgaaaacg tgcattgtaa tcaaatttag cagtcactgt gatggcttga ccttgttgga
     3361 ctatttcgtc atgaaccata tgccaagggt caaaaaagct tgcattaaat ttattggtaa
     3421 aatgttcaaa gtgctttttg atggggggtg ttaacatagg agtttttata acagcttcca
     3481 tgtcaggaca ttgggcatag ctaaaaaaag gataaaaaca ggcaaaaata aaagggataa
     3541 cacgcataaa acatcgttcc tagatcatgg ttttattatg acatgaacca attcaagcgt
     3601 cttgttttca agtgttgttt aaaagaggcg ataacatcgc ctctttaatg ctttaaatat
     3661 tacccataaa caagcgtttc atcacctttt ctttgaggct atgggtttcg ttaaaaccca
     3721 tacgatctaa ataacgatgt actttgtcat catcttcatc tttaaaaatc gagctaacca
     3781 ccatttttaa taattgcaga ggatgctcag tgcttgtttt taaggattgt aaggctttga
     3841 caatgtttaa ttcatctttg gtaaagtctg taccaaaagg aaagctcggt aaatgctgca
     3901 taaacggttg caaacgttcc gctaaggctt caggcgtatt gttggtatag gctttgggaa
     3961 tcacataatc ggccgataat ttacctttgc tcactgccca ttcacgcaat tcttcttggc
     4021 aacgagaatc actgatattt aacaaggctt taatgatgtc gctgtcattt ttgccacgca
     4081 aatcggcaat accatattcg gtaacaaata tatcgcgtaa atggcgtgga atggtaatgt
     4141 ggccataatt tttgacaatg cttgagcgca cctcaccatc aaccatacga tgactacgca
     4201 acattaacac tgagcgtgca tcgggcaatt catgcgcttg agcaacaaag ttatattgtc
     4261 cacctacgcc actcactaca cgcccatctt ctaaagcatc tgataccgcc gcgcctgata
     4321 aagtcgccat catacaggtg ttaataaaac gtgctttgac acgctgagca cgttttaggt
     4381 tctcatcgcc ataacgatca ccatataact ggttaataaa ggcaatggtg ctcatattaa
     4441 ttaaattgag ttgatcgtca ggcatatcac gcaaggcttg ataaaagtcg gcagggccta
     4501 aaaagaagcc accgtgcata atcacgccat tttctaaacg ttggcctaaa cataaactag
     4561 cgatctgttg acaggcttca gcattgtcta aatcaccact aatttgttgc tcaccaacga
     4621 ttaatacgcc atcttcaacg ctgcaatcat cgctaaaaat gccaaaacgg gttaataagg
     4681 caacgtcctc acctgttaag ggagagctaa tgagatcttg ttcgcgtagg gttttgaggg
     4741 tatctaaaga aacagtttcg ctaattaagc cttgattgag cagtttttgt aagcctgtat
     4801 ggttatagac ttggcggcga ataatattgg cttgaatcag ctttaaaaag ccattaacaa
     4861 acatttcgct acagccatac aagccttgtt taaagggggt taagtctaac tccgcagaca
     4921 gtggtaaatt gtggtttaag cggcgaatca tattttggta agtgacatta tcacgatcac
     4981 gcagcattaa accttggaca atcgcgtccc ccaaagagcc aataccgatt tgtaatgtac
     5041 cgccatcttg aactaaagaa cttgcatgta aaccaatggc ataatcactt aaacctactt
     5101 tcatgttagg ggtggagaat agggtgtgtg tgcctgctgc atcttcaata atcatatcaa
     5161 attgatcggc agagacttcg gcatcattag gcataaatgg cagcttatgg ttaatcacac
     5221 ctacgacaaa aatacgttca cctgcctctc gacgcggttg taataagtct aataaatctt
     5281 cgcttaaatc tggattacac gataagctat aacgacgtgt gccatcggct tcgtcacgta
     5341 ccgcaataga ttggcaaatg acattaacac cgttgtgtgc catgtcgcgg gcaatatggg
     5401 tgtagttgct gttgatatag tgttgttgtg cattggcatt attgagccaa tcacccgatt
     5461 ttaaaaagaa ctcattaacg ctaatgttat tgggaacttt attgttttta aggtcagtga
     5521 catagtttaa atcgggataa tcgccaaaga cacgttcaac aaacggctct aaaaagcgtg
     5581 cttctaagtc actgctagcc gttggtttgg ttaaggataa ggcactaaag atatgcaaac
     5641 atcgctcagg catgctgcta atccgttgat acatggcgtt aataaaagca ttgggtttgc
     5701 ccaagcctaa aggtgcgcca agtcgaatat cgccatcgat tttattaata atatcagtga
     5761 tggcttgttc gatagagtta tagaacttaa cggacataac agtaactcct agaggaaaaa
     5821 gataatttat tgtgacagtt tgtttataga gcaaaaactc ttaatttttg taaggtttat
     5881 gtctttgagc ataccccaat agcctttttt ggcaagagat ttttgatgac taataagttt
     5941 gatatttgtg tgatagaaga agaaatagta tgtcgtgcag ggtgtgccgc ctgttgtatt
     6001 gcgccttcta tttcttcggc tattcctgat atgccaaatg gtaaacctgc agatgtgcct
     6061 tgtgtgcaat taaatgaaca aggtttttgt aaattgtttg gtttaccaga acgtcctaaa
     6121 gtatgtagca gtttaaagcc aacttttagt atgtgtggct caac
//
LOCUS       DAOQMB010000102         6155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5484_length_6155_cov_3.982459, whole genome shotgun sequence.
ACCESSION   DAOQMB010000102 DAOQMB010000000
VERSION     DAOQMB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6155
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5484_length_6155_cov_3.982459"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1451)
                     /locus_tag="PLY05_05090"
     CDS             complement(213..1451)
                     /locus_tag="PLY05_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02713.1"
                     /translation="MAIDLYVIIVSEHNLAELRACLYLQPTTILMVSSHKMQAAAQRL
                     LGVLQKQLPHTKVEVLTSTTDTPFSGDYLQENQQWLERIFLPKLAQIKPSSAQLNMTG
                     GTKILAYLLTRIYPWQEIHYQPLADTIPLERFYTQNDSPHLLPTIDLATAATSDISPD
                     NHALLYMDYVRPHSPNIIRKHPDSLAIALLRLETQQANNPHQGLGAFIALFEQAWSLP
                     TQEPFVNMPIPPNTHLDESLLARLNNLYIGSHAAPLTRTPEGLQIPAAHHKKYTDWRK
                     WISGDWYEQLIEQWLLDYGIDKKHLLSNVQLSNKTDPQGQESDTLLQYKNKLYVIEIK
                     ADVPQSKQLGDMENQLSSLAMQLGKVENVLILSPAIRRRYAPEQWLRFELRCRNKNVK
                     LCVADTQSSFINQFFYSSKP"
     gene            complement(1464..4118)
                     /locus_tag="PLY05_05095"
     CDS             complement(1464..4118)
                     /locus_tag="PLY05_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02714.1"
                     /translation="MHALCLPFVSGDLPANFSAISLTLDTAIHSLSAAKTLLAQQTQP
                     SYIAFSPKTPNLLVLACQQLWPDARYLLQEEQAWLELPSGEYWTTTNDNTTTESTEPV
                     LDTVSPQEPTESTPPQVQVFPSTTTINTNSPKSDNYLLDKVFKLASWGENIPENRAQL
                     DELFVELLKRSERNPRLIKRAQKKRDLGALAAKVWIEAEKRLKSTGYTAEFEAWFNQQ
                     VNNADGSTAFFLKNKLQKWQTWYRQRKQKFQTNAICTLPELRFSDHHPNSLRHLTPHH
                     HWHILIDETGSEFDNSKQNISDKTLGRMVALAIPYDKVSLPTLKKNFHATSESDALLD
                     KTIKTLLDNPVGIVGITVNDDLLGKSSRWFSGIYMLMRLVLRLLPIQGGQGKVEFFIE
                     QRGGFDAQMSLLPIQQLLETELHALDKERFQHISLILQFSDKNHHPANGYVDAVAHMW
                     AAGSANSKTRLKHSALKGHCLLEPHHDVIDRTYAAIDQKLDLSAKDWYQLVSSISHEP
                     SSSLLHSVLQQLGRQTQQQADIWHRYLDYISQLLKDKNYRLLPLKSALDWLASYAPKT
                     LSLPPMLTLHWYMARLACANHLGKSDISLIEKSLVLGSQLLEENAPDVCHLHLRIAVA
                     ATNLFEFNAAQQVLDFWKDQAPRVMGLANYGKYLSSLGQIAAFKGNLSQAITYFDQAL
                     ETFAQLSDKKDKAKQQQQTQIYRLIAFMDMPEHSDSQVKAQLEQFFMNTLDKVAQRLS
                     MGDDNSRFEHHVLLRAMITRPALFEESYLNNASEWQLGDAHPWPLICAYRAWLLHAKN
                     PNQAVLWMQRAISMCQNEGTTLKWMAEVLTVMAQKQGIDAAHSIDLSDLKNELVHAPW
                     LALQALQQANTPQTIHKAFVDCLPFNFH"
     gene            complement(4284..5414)
                     /locus_tag="PLY05_05100"
     CDS             complement(4284..5414)
                     /locus_tag="PLY05_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC02715.1"
                     /translation="MTTHPFSNNSIIESLISQAYKNAQSQIEGWQNTRIKIAIAGMSG
                     AGKSSLINAILNQKIAPVGVIETTVEAKSYDSEHGITFVDLPGCGTTQFPFDSYIQKT
                     NLASFDAIILVTSNRFYENDVQLYAQIIQQLNIPCFVVRSKIDLAINDGLEDNGLTTE
                     QVCQMVTDNIYQNLKPHRPKKVYLVSNRYRQEYDLQCLIDDICLSLEGLKSAKFKAES
                     AAYSKKAIYSKRKVIEEIAFHYAISAAINGLNPVPALNVSVDAGILLSMSQHILRFYG
                     LTEQQMEHFMGAKQQKVQLQALKQGILRWSAQFLAKESILLILKRLSTKLASKTLASW
                     IPMVGQIVSAGIGFKMTISFATELIDESEKKSLELLQTLLEN"
     gene            5749..>6155
                     /locus_tag="PLY05_05105"
     CDS             5749..>6155
                     /locus_tag="PLY05_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02716.1"
                     /translation="MQLPVFLDIEASSLSSSSYPIEIAWNDHTGHIQSYLISPMGIKS
                     WTDWSAKAEKIHGISQDTLITQGQSPTSVAQIIHQQLSKQVVYSDNPDYDEYWLGELF
                     KKSAGLSLYVTVKSIDVLLTKILSPQFMEPTQL"
BASE COUNT         1760 a         1158 c         1300 g         1937 t
ORIGIN      
        1 gccttgattg agctgattga tggcattgcc agacatagaa ctcgaggtgt ctagcaccaa
       61 catacaagcg cagcgaggag aaggattgtc gattaaatcg ttgttggtca ttaaagcaaa
      121 gtttggcatg gcgttagctc cagtggttaa gtgaataacg ctatactaga gaaaaacgac
      181 taggccgaac cgttttgcaa gcttgcgaaa tgttagggtt tggaggaata aaaaaattga
      241 ttgataaatg aagattgagt gtcagcaacg caaagcttaa cgtttttgtt tctgcagcgc
      301 aactcgaatc ttaaccattg ttctggtgca taccgacgac gaattgcagg cgatagaatc
      361 agtacgtttt caactttgcc caattgcatg gccaaagagc ttaattgatt ttccatatcg
      421 cctaattgtt tgctttgtgg aacatctgct tttatttcaa tgacatataa tttatttttg
      481 tattgtagta gcgtgtcgct ttcttggcct tgagggtcag ttttgttgct taattgcaca
      541 ttacttagca aatgcttttt atcaatgccg tagtctaata accactgttc aattaattgc
      601 tcataccaat cacccgaaat ccatttgcgc cagtctgtat attttttatg gtgtgccgca
      661 gggatttgca agccctctgg cgtgcgtgtt aaaggtgcag cgtgtgaacc aatatacaag
      721 ttattgagtc gtgctaacaa cgattcatcc aaatgtgtat taggaggaat cggcatgttt
      781 acaaacggtt cttgcgtagg caaagaccat gcttgctcaa ataacgcaat aaacgcacct
      841 aagccttgat gagggttatt ggcttgttgt gtctctaaac gtaacagggc gatggctagg
      901 ctatcggggt gttttcggat gatattaggg ctatgtggtc gcacataatc catataaagt
      961 aaggcatgat tgtcgggtga aatatcactt gttgcagcag ttgcaaggtc tatggttggt
     1021 agcaaatgtg gactgtcgtt ttgggtataa aaccgctcta agggaatagt atcagccaaa
     1081 ggttgataat gaatttcttg ccaaggataa atgcgagtga gtaaatacgc taagattttg
     1141 gtaccgcctg tcatattgag ctgtgcagag gatggcttaa tttgcgctag tttaggcaaa
     1201 aatatacgct ctaaccattg ttgattttct tgtagataat caccactaaa tggcgtatca
     1261 gttgtagagg tcaacacctc caccttggta tggggtaatt gcttttgcaa tacccctaat
     1321 aagcgttgtg ctgccgcctg cattttgtga ctgctaacca ttaaaatagt cgtgggctgc
     1381 aaatataagc aagcgcgtag ctcggccaag ttatgctcag aaacaataat cacatacaaa
     1441 tcaatagcca tgttttgctc taattagtga aaattaaagg gcaaacaatc aacaaacgcc
     1501 ttgtgtatgg tttgtggcgt attggcttgc tgtaatgctt gcaaggctag ccacggtgca
     1561 tgcactaatt cgttttttaa gtctgataaa tctatgctgt gagcagcatc aatgccttgt
     1621 ttttgcgcca taacggttaa tacttcagcc atccatttaa gcgttgttcc ctcattttgg
     1681 cacatgctaa tcgcacgctg catccacaaa accgcctgat taggattttt ggcatgcagt
     1741 aaccatgcgc ggtaggcaca aattaaaggc caaggatgag catctcctaa ttgccactca
     1801 ctggcgttat ttaagtagct ttcttcaaat aacgcagggc gggttatcat ggcacgtagc
     1861 aaaacatgat gctcaaagcg gctattatca tcacccatac ttaatctttg cgcgactttg
     1921 tctaaggtgt tcataaaaaa ttgttctagc tgggctttaa cttggctgtc actatgctcg
     1981 ggcatatcca taaaggcaat caaacgataa atttgcgttt gttgttgctg cttagcttta
     2041 tcttttttgt cacttagctg cgcaaaagtt tctagtgctt ggtcaaaata ggtgattgct
     2101 tgtgataaat tgcccttaaa ggccgcaatc tgccctaaac tagataaata tttaccatag
     2161 ttagctaaac ccattacccg tggggcttgg tctttccaaa aatcaagcac ttgttgtgca
     2221 gcattaaact caaacagatt agtggctgca acagcaatgc gtaagtgcaa atgacataca
     2281 tcgggtgcat tttcttctaa taattgactg cctaatacca agcttttttc tataaggcta
     2341 atgtcacttt ttcctaaatg attggcgcaa gctaatcgcg ccatatacca atgcagggtt
     2401 aacatgggcg gcaaacttaa ggttttagga gcatagcttg ccagccagtc taaggccgat
     2461 ttaagcggca atagacgata gtttttatct ttgagtaatt ggctaatata atccaagtaa
     2521 cgatgccaaa tatcggcttg ttgttgggtt tgacgaccta attgttgcaa tacactgtgt
     2581 aataaactgc tgcttggctc gtggctaata ctactgacta attgatacca gtctttggca
     2641 cttaaatcga gtttttggtc tatagcggca taagtgcggt caatcacatc atggtgtggc
     2701 tccaataaac aatgcccttt taaggcactg tgtttaagcc gtgttttgct atttgccgat
     2761 cccgcagccc acatatgcgc cacagcatca acataaccat tagcaggatg atgattttta
     2821 tcagaaaatt gtaaaatgag cgaaatatgt tgaaaacgct ctttatctaa ggcgtgtaac
     2881 tctgtttcga gtaattgttg aatgggtaac aaggacattt gggcatcaaa gccaccgcgc
     2941 tgttcaataa aaaactcaac tttaccttgt ccgccttgaa tgggtaataa tcgcaatacc
     3001 aaacgcatta gcatataaat gccgctaaac cagcgtgaac ttttgcctaa taaatcgtca
     3061 tttacagtaa tgccaacaat ccccactgga ttatctaaaa gggttttgat ggttttatct
     3121 aacaaggcat cgctttctga cgtggcatga aaattctttt ttaatgttgg caagctaact
     3181 ttatcatagg gaatagctaa ggccaccatg cgtcctaagg ttttatctga aatgttttgt
     3241 ttgctattgt caaattcgct gcctgtttca tcaatcaaaa tatgccaatg atgatgaggt
     3301 gttaaatggc gtaagctgtt gggatgatgg tcagaaaacc tgagttcagg tagcgtacaa
     3361 atagcatttg tttgaaattt ttgttttctt tgcctgtacc atgtttgcca tttttgaagt
     3421 ttattcttca aaaaaaaggc agttgagcca tcggcattat taacttgttg gttaaaccat
     3481 gcttcaaatt cggctgtata gcctgtactt tttagtcttt tttccgcttc tatccatact
     3541 ttagcagcca gtgctcccaa gtcgcgtttc ttttgagctc tttttatcag tcgcggatta
     3601 cgttcgctgc gttttagcaa ttcaacaaat aactcatcta actgagcacg attttctgga
     3661 atgttttctc cccatgaagc aagtttaaag actttatcta acagataatt atctgactta
     3721 ggactattag tgtttattgt ggttgttgag gggaatacct gtacttgagg tggtgtactt
     3781 tctgttggct cttgaggact cactgtgtcc aaaacgggtt ctgtactttc tgtcgtggta
     3841 ttgtcgtttg ttgtcgtcca gtattcgcca ctgggcagtt ctagccaagc ctgttcttct
     3901 tgcagcaaat aacgtgcgtc tggccaaagc tgttgacaag ccaataccaa taaattgggt
     3961 gttttgggcg aaaaggcaat ataactgggc tgcgtttgtt gtgccaatag tgttttagcg
     4021 gcggatagag agtgaatggc tgtgtctaat gtgagtgata ttgcgctaaa gtttgcaggt
     4081 aaatcacccg agacaaaagg taagcataag gcgtgcatac aggcttccaa gcttgattat
     4141 taggggtatt agcctgctaa tttagagaat gagcagacta tttgtcaatt ttaaaatacc
     4201 cactcaccaa taagattgtc acaaaaaacc tccactaacc acaggcaaca cgcagctaat
     4261 ggaggcaggg cattgaaaaa acattaattt tctagcagtg tttgcaatag ctccaatgat
     4321 tttttctcgg actcatcaat taactctgta gcaaagctaa tggtcatttt gaagccaata
     4381 cctgcagaga cgatttgccc aaccatcgga atccaactgg ccagtgtttt ggaggctaat
     4441 ttggtcgata agcgtttaag aattaggaga atactttctt ttgctaaaaa ttgtgctgac
     4501 caacgaagaa tgccttgctt gagagcttgt aactgaactt tctgttgttt agcccccata
     4561 aaatgttcca tttgctgctc ggtaaggcca taaaagcgca aaatatgttg agacatgctc
     4621 agcaaaatac ctgcatcaac actgacatta agggcaggaa caggatttaa gccattaata
     4681 gcagcagaaa tagcataatg aaaggcaatt tcttcaataa ctttacgttt gctataaata
     4741 gctttttttg agtaagcggc tgattcggcc ttaaacttcg cgctttttaa accctctaaa
     4801 gacaagcaaa tatcgtcaat taagcactga agatcatact cttggcgata acgattagat
     4861 actaaataca cttttttagg acgatgaggt tttaggtttt gatagatatt atctgtcacc
     4921 atttggcaaa cttgttcggt ggttaggcca ttgtcttcta atccatcatt aatggccaag
     4981 tcaattttgc ttcttaccac aaaacaagga atattgagct gttgaataat ttgcgcatac
     5041 agttgaacat cattttcgta aaaacgatta gaggtgacca aaataatggc atcaaaactg
     5101 gctaagttgg ttttttgaat ataactatca aacggaaatt gtgttgtacc acagcctggt
     5161 aaatcaacaa aggttattcc atgctcactg tcataactct tggcttcaac ggtagtttcg
     5221 ataacaccaa caggtgcaat tttttggtta agaatggcat taattaaaga agatttgcct
     5281 gcaccactca tgcctgcaat cgcaatttta atgcgtgtat tttgccaacc ttcaatttgt
     5341 gattgtgcgt ttttataggc ttggctaatt aaagattcga tgatgctgtt gttagaaaaa
     5401 ggatgcgttg tcatgatgag taaccttatg ttaagaagtc gtgatggcta ttattaaccc
     5461 aaaaatagat aaggtactta aatgcaagct tgtgtgatgg ctaatcgtcg tgcaaaaaaa
     5521 ttatgctact aactttgtaa tagaaggtga attatgggct ttgagttgat tgcttttttt
     5581 gccatgcttt tttgggtagc cttatttttg tatggcacag tcggttttgc catttgggag
     5641 catttaattt tggtgaaatt aggtttattg ctattaatat tcatatattt aagatttttt
     5701 tgggatccta ctgagtagta gaattgagta tttgcagggt gattaaacat gcaacttcct
     5761 gtgtttttag atatagaagc ctcgagtttg tctagcagtt cctaccctat agaaatagcg
     5821 tggaatgacc acacaggcca tatacagtca tatttaattt cgcctatggg cataaaatct
     5881 tggacagatt ggagtgccaa agccgaaaag atacatggta ttagccaaga taccttaata
     5941 acacaagggc aatcaccaac atcggtcgca caaataattc atcagcaact cagcaagcaa
     6001 gtggtttata gcgataatcc tgattatgac gaatactggc ttggcgaatt atttaaaaaa
     6061 tcagcagggc ttagtttata tgttacggtt aaatcaatcg acgttttgct aacgaaaata
     6121 ttaagccctc agtttatgga gccaacacag ctaat
//
LOCUS       DAOQMB010000103         6134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5516_length_6134_cov_14.657838, whole genome shotgun sequence.
ACCESSION   DAOQMB010000103 DAOQMB010000000
VERSION     DAOQMB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6134
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5516_length_6134_cov_14.657838"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLY05_05110"
     CDS             <1..690
                     /locus_tag="PLY05_05110"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNC02717.1"
                     /translation="PQDMRYWQASWQTGQVIKESLDSQSSIGFKQVFANLWARSGVLT
                     LIISLICVGVYLVFAAVGRPVFEALNYPQDIKHTQEYWRLLTPVFIHFNFAHLLFNLI
                     WWWDLGGLIERTQSKMQLLGVFLATALLSNLGQSLAFGNANNFGGLSGVVYGLLGYVW
                     LYPLANPKVGFRLNTAIVAFMIGWLVVGYSGILDNIIGKMANDAHLIGLLTGIILGCG
                     FGLLHRFTQQK"
     gene            complement(720..2390)
                     /gene="rsxC"
                     /locus_tag="PLY05_05115"
     CDS             complement(720..2390)
                     /gene="rsxC"
                     /locus_tag="PLY05_05115"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNC02718.1"
                     /translation="MTVQRAPYKLIGGVHVPEHKQSSTQSPIKPLALAKRLIIPMLQH
                     IGEEAKPIVNIGEHVLKGQKIGEVRAFVATPVHATTSGTVVDIGLYSVDHPSQLDALC
                     VVIEADGKDEWAAHTGIKDYHLAEAQTLLAIIREAGIAGMGGAGFPTGSKLQVKQQTP
                     VDIVVINAMECEPYITADDLLMRERAAEIVEGANIVKQILGAKKIIIGIEDNKPQAIN
                     AMQQASQSQAHIQVMSCQSKYPSGAARQTVYILTGRETPATGRTTDVGVFCLNVGTAA
                     AIYRAVVHGEPSLSRITTVTGLGVQQAANYDVPFGTPIEHLLNQAQAQWSKISRLIVG
                     GPMMGYSLPQVQIPVVKTTNCIIAATLEDFPPPPPEQACIRCGDCAQVCPVVLLPQQL
                     LWFAKNGEFDKAEQYHLDDCIECGACAYVCPSSIPLVQYYRYAKGEIATQKQEAEKAE
                     RARLRFEAHKARLAKEEADKEARRIAREQAAAQAREEAAQQAALASQQGAAPPAKDSK
                     ALMIAAATARSNLKKAEKRLLAAQQAGEDTSALLAEIEQLKAEVASYQ"
     gene            complement(2387..2992)
                     /gene="rsxB"
                     /locus_tag="PLY05_05120"
     CDS             complement(2387..2992)
                     /gene="rsxB"
                     /locus_tag="PLY05_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HNC02719.1"
                     /translation="MSALFTIIIAIVILLIIGLLAGALLGFASEKFKVEANPLVDQID
                     ALLPQTQCGQCGHPGCRPYAEAIANGEAINHCVPGGQDTINQLAALLNVEAIPLDSEY
                     GVESEVKKVAFIREAECIGCTKCIQACPVDAIIGAAKHTHTVISSECTGCDLCVEPCP
                     VDCIDMIPIAVTRQTWHWQAPKGEGNSPVEHPIKLIPLKQL"
     gene            complement(2989..3570)
                     /gene="rsxA"
                     /locus_tag="PLY05_05125"
     CDS             complement(2989..3570)
                     /gene="rsxA"
                     /locus_tag="PLY05_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNC02720.1"
                     /translation="MNDYLLLLIGTILVNNFVLVQFLGLCPFMGTSKKLSTAAGLAIA
                     TTFVLTLSSIFTYAVDALILQPFDLAYLHTLSFILVIAVTVQFTELVLHKTSPALYRV
                     LGIYLPLITTNCIVLGVALLNIRQANPSFIKALLTGLGAGLGFALVLVLFAAMRERIN
                     VADVPKPFRGSAIALVTAGLMSLSFMGFAGLVK"
     assembly_gap    3592..3691
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3692..5188
                     /locus_tag="PLY05_05130"
     CDS             <3692..5188
                     /locus_tag="PLY05_05130"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC02721.1"
                     /translation="LAADIKAKKISSRAVVEAHIAQVKCVNPRLNAIIYDRFAQALRE
                     ADQADAVLAQTQDPSTLPLLFGVPCTIKDTFEFEGMPQAVGMVSRKDIFGQQDAYAVK
                     VLRQAGAIPLGVTNTPELAMWYETYNRVYGRSKNAYDRGRITGGSSGGEGAIVGAGAS
                     PFGLGSDVAGSIRMPAFFNGVFGHKGSPLLIANDGHFPSAHGQTLNYLSSGPLCRKAE
                     DLEPLVRLLAGKNSHKLKSVKDVNISKLRVITIAPERGPKATFDILDAQQRAVDALVA
                     QGASHITVDLPLVDEAFMIWSSLLADSSTPEHSFTAMLFGEYHVKHPVKALLNMAVKG
                     KKSPHTLPLLVLTLLEQFPNLATGSRKHFVEKGHQLKQQLNDLLADDGVLIMPVFPEV
                     APRHGAPIFKPFDFVDCAIVNAMELPATAVPMGLNEKGLPTGIQVVANQDNDHLSLAC
                     AVALEQATGGWFPPWQSWLADLPNSVLTVDNAQSVKNSVQKRGRKPKN"
     gene            5305..>6134
                     /locus_tag="PLY05_05135"
     CDS             5305..>6134
                     /locus_tag="PLY05_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC02722.1"
                     /translation="MQPIQGVAKVGDIELCYERWGNSHDPAILLIMGLGAQMLIWPDS
                     FCQTLVDKGYQVIRFDNRDIGLSSKIKKRSHKQSFLLSMARFQLGLPNPAPYDLYDMA
                     ADTVGLMSVLGIDKAHLIGASMGGMISQIIAAKYPNRVLSLGILFSSNNQPLLPPPAP
                     KALMSLISRPDGNDNSREALLDHSVNLFTTIGSPSYRTHPEDLRAFANKLMDRSYHPA
                     GVKRHFLAVMGTGNLKHLARHIHVPTVVVHGREDKLLRPACGKAIAKSIKGAHLELIK
                     G"
BASE COUNT         1717 a         1237 c         1311 g         1769 t
ORIGIN      
        1 cctcaagata tgcgttattg gcaggcttct tggcaaacag gtcaagtgat taaagaaagt
       61 ctggatagtc aaagtagtat aggatttaaa caggtctttg ctaatttgtg ggcaagatca
      121 ggcgtattga ccttaataat tagtttaatc tgtgtggggg tttatcttgt ttttgccgca
      181 gtgggtaggc ctgtgtttga ggcattaaac tatccgcaag atattaagca cactcaagaa
      241 tattggcgat tattaacgcc tgtttttatt cattttaatt ttgctcattt attatttaac
      301 ttaatttggt ggtgggattt aggcggcctt attgagcgaa cccaatcaaa aatgcaactg
      361 ttaggtgtgt ttttagccac ggctttatta agtaatttag ggcaatcatt agcctttggt
      421 aatgctaata attttggtgg tttgtcaggc gttgtttatg gattattagg ttatgtgtgg
      481 ctgtatccac ttgccaatcc taaagtcgga tttaggttaa atacggcaat tgttgcattt
      541 atgattggat ggttagtcgt tggctatagt ggtattttgg ataacattat tggcaaaatg
      601 gccaatgatg cccatttaat tggcttatta acaggtatta ttttaggctg tggttttggg
      661 ttgttgcata gatttactca acaaaaataa tagtggctgt tgacgttttg cgagttgtcc
      721 tattgataac tcgcaacttc ggcctttaac tgttcaattt cagccaataa agcagaggta
      781 tcttcgccag cctgttgtgc ggccaataag cgtttttcgg ctttttttag gttgctacgg
      841 gcggttgctg cggcaatcat taaggctttg ctgtctttgg cgggtggcgc agcaccttgt
      901 tggcttgcta aggctgcttg ttgggctgct tcttctctgg cttgagccgc tgcttgttca
      961 cgcgctatac gccgtgcttc tttgtcggct tcttctttgg ctaaacgggc tttatgtgct
     1021 tcaaagcgta aacgtgcgcg ttcagctttt tcagcttctt gtttttgagt ggcaatttca
     1081 cctttggcat aacgatagta ttgcactaag ggaatgctac tcggacagac ataagcacac
     1141 gcgccacatt caatacaatc gtctaaatgg tattgttcgg ctttatcaaa ctcaccattt
     1201 ttagcaaacc ataacaactg ttgtggcaac aacaccacag gacatacttg cgcgcaatcg
     1261 ccacaacgaa tacaggcttg ctcaggcggt ggcggtggaa aatcctctaa tgttgcagca
     1321 ataatacaat tagtagtttt aaccacagga atttgtactt gtggcaagga gtagcccatc
     1381 attggcccac caacaattaa acgcgaaatt tttgaccatt gagcctgtgc ttgatttaac
     1441 aaatgctcaa taggtgtgcc aaagggcacg tcataattgg ctgcttgttg tacgcctaaa
     1501 cctgtgacgg tggtaatccg cgataaagac ggctcaccat gcaccactgc acgataaatg
     1561 gcagcagcag tgccaacatt taaacaaaat acgcccacat cggtggttcg acccgtcgca
     1621 ggggtttcac gacctgttaa aatatacaca gtttgtcgag ctgcccccga agggtatttg
     1681 ctctgacagc tcatgacttg aatatgcgct tgagactggg acgcttgttg catagcattg
     1741 atggcttgcg gcttgttgtc ttcaatacca ataataattt tttttgcgcc taaaatttgc
     1801 ttaacaatat ttgcgccttc aacaatctcc gccgcgcgtt cacgcattaa taaatcatca
     1861 gcggtaatat atggctcaca ctccatggca ttaatcacca caatatccac aggtgtctgt
     1921 tgtttgactt gtagttttga gcctgttgga aagccagcac cgcccatacc tgcaatgcca
     1981 gcctcacgaa taatcgctaa taaggtttgg gcttcggcca aatgataatc tttaatacct
     2041 gtatgggctg cccattcatc tttgccatca gcctcaataa ccacacacaa cgcgtctaat
     2101 tgtgaagggt ggtcaacaga atacaagcca atatcgacca ctgtgcctga tgtggtggca
     2161 tgaacaggcg tggccacaaa tgcgcgcact tcaccaattt tttgaccttt taatacatgc
     2221 tcaccaatgt tcacaatcgg ttttgcttct tcaccaatgt gctgcaacat gggaataatc
     2281 aaacgtttag ctaaggctaa aggcttaatt gggctttggg ttgagctttg tttgtgttca
     2341 ggcacatgaa caccaccaat caacttataa ggtgcgcgtt gtacagtcat aattgtttga
     2401 gtggaatgag tttaatggga tgttcaacag gactattacc ttcgccttta ggtgcttgcc
     2461 aatgccatgt ttgacgggta acggcaatcg gaatcatatc aatacaatca acagggcaag
     2521 gctctacaca caaatcacag cctgtacatt cgctgctaat caccgtatgg gtgtgttttg
     2581 ctgcaccaat aatcgcatca acagggcaag cttgaataca tttggtacaa ccaatacatt
     2641 cggcttcacg aataaaagcg acttttttaa cttcagattc aacaccatat tcactatcta
     2701 aaggaatggc ctcaacattt aacaaagccg ctaattgatt aatagtatct tgaccaccag
     2761 gtacacaatg attaatcgcc tcgccattgg caatagcttc ggcataaggt cgacaaccag
     2821 ggtgaccaca ttgaccgcat tgagtttgag gtaatagggc atcaatttgg tcaactaagg
     2881 gattagcttc gactttaaat ttttctgacg caaagcccaa taacgcgcct gctaataaac
     2941 caataattag caaaataaca atagcaataa taatggtgaa taacgcactc atttcaccaa
     3001 ccctgcaaaa cccataaagg ataatgacat taaacctgcc gtgactaaag caatagcact
     3061 gccacgaaaa ggcttaggca catctgcgac attaatacgt tcgcgcatgg cagcaaacaa
     3121 cactaacact aaggcaaagc ccaaacctgc acctaaacct gttaataatg ctttgataaa
     3181 cgaaggattt gcttggcgaa tatttaataa agcaacacct aacacaatac agtttgtggt
     3241 aatcagtggt agataaatgc ctaacactcg atagagggca ggactagttt tgtgcaatac
     3301 taattcggta aattgtacgg taacggcaat gactaaaata aagcttaaag tgtgcaaata
     3361 ggctaaatca aaaggctgca aaattaaggc atcaaccgca taggtaaaaa tggacgataa
     3421 ggttaaaaca aaggtggtgg cgatggctaa acctgcggcg gtgctgagtt tttttgatgt
     3481 tcccataaat gggcataaac ccaaaaattg taccaagaca aaattgttga ccaaaatcgt
     3541 gccaatcagt agcagcaaat agtcattcat gggagtctga gccttttaaa tnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttagctgcg gatattaaag ccaaaaaaat
     3721 cagttctcgt gccgtggttg aagcacacat tgcccaagtg aaatgtgtta atccacgtct
     3781 taatgccatt atttatgacc gttttgccca agcattacgc gaagcagacc aagcagatgc
     3841 tgttttagca caaactcaag acccctcaac cttaccctta ttatttggcg tgccatgtac
     3901 cataaaagac acctttgaat ttgaagggat gccacaagcc gtgggcatgg tgtcgcgtaa
     3961 agatattttt ggtcaacaag atgcctatgc cgttaaggta ttacgtcaag caggagcaat
     4021 tcccttaggt gttaccaaca cgccagaatt agccatgtgg tacgaaacct ataaccgtgt
     4081 ttatggtcgc agtaaaaatg cctatgatcg tggtcgaatt actggtggct catcaggtgg
     4141 tgagggcgca attgttgggg caggcgcaag tccatttggc ttaggttcag atgtggcagg
     4201 ttcaattcgt atgccagctt tttttaatgg tgtctttggc cacaaaggct cacccttatt
     4261 aattgccaat gatggtcact ttcccagcgc acatggccaa acactcaatt atttatcgtc
     4321 agggccattg tgtcgtaaag ctgaagattt agaaccttta gtgcgtttat tagcaggtaa
     4381 aaacagtcat aaacttaaat cggttaaaga cgtaaatatt agcaagctac gggttattac
     4441 cattgcgcca gaacgtgggc caaaagccac ctttgatatt ttagatgcgc aacaacgtgc
     4501 agtggatgcc ttagtcgcac aaggcgcaag ccatattacc gtcgatttgc cgctcgttga
     4561 tgaagctttt atgatttggt cgagcttgtt agcagattca tccacgcccg aacattcctt
     4621 tacggcaatg ttatttggcg agtatcacgt taaacatcct gtaaaagcct tattgaatat
     4681 ggcagtaaaa ggtaaaaaat cgccacatac cttgccttta ttggtgttaa ctttattaga
     4741 acaatttcca aatttagcca caggttcacg caagcatttt gttgaaaaag gtcatcaact
     4801 gaagcagcaa cttaatgact tattggcaga tgatggcgtg ttgattatgc ctgtttttcc
     4861 cgaagttgct cctcgtcatg gtgcgccaat ttttaaaccc tttgattttg ttgattgtgc
     4921 gattgttaat gccatggaat tacctgcaac tgctgtacca atgggattaa acgaaaaagg
     4981 cttacccaca ggtatccaag tggtagctaa tcaagataat gaccatctat ctttggcttg
     5041 tgccgtcgct ttagaacaag caacaggcgg atggtttccc ccttggcagt catggttggc
     5101 tgatttgcca aacagtgtgt taactgtaga taatgcccag tccgttaaaa atagtgtaca
     5161 aaagcgtggt cgtaagccaa aaaattagga cttacgcatt tcgctgaaat tagcgcgttt
     5221 tgtgaacata aaggcattga aaatgcctga aatgcgaaca aataagcgat aaccgcgtaa
     5281 gtccaaaaag ttaggagttg aataatgcag ccaattcaag gtgtcgcaaa agttggtgat
     5341 atagagttat gttatgagcg ttggggcaat agccatgacc cagccatttt gttaattatg
     5401 ggtttaggcg cacaaatgtt gatttggcct gatagctttt gccaaacttt ggttgataaa
     5461 ggctatcaag ttattcgatt tgataatcgt gatattggtt tgtctagtaa aattaaaaaa
     5521 cgtagtcaca aacaaagctt tttattgtca atggcacgtt tccaattagg gctacctaat
     5581 cccgcgcctt atgatttata tgatatggct gctgatacag tgggcttaat gtcggtttta
     5641 ggcattgata aagcacactt aattggcgca tcaatgggcg gcatgattag ccaaattatt
     5701 gctgccaaat atccaaatcg ggtcttaagt ttagggattt tattttccag taataatcag
     5761 cctttattac caccacctgc acccaaagca ctcatgtcgt tgattagtcg acctgatggc
     5821 aatgataact cacgcgaagc cttactcgat catagtgtta atctattcac tacgattggc
     5881 agtccatctt atcgtacaca tcctgaagat ttaagagcat ttgcgaataa actcatggat
     5941 cgcagttatc atccagcagg ggtgaagcgt catttcttag cagtgatggg aacaggtaat
     6001 cttaaacacc ttgctcgtca tattcatgtg ccaacggttg ttgtgcatgg tagagaagat
     6061 aaattgttgc gtcctgcttg tggcaaagcc attgccaaaa gtattaaagg tgcacactta
     6121 gaacttatta aggg
//
LOCUS       DAOQMB010000104         6133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5521_length_6133_cov_15.579631, whole genome shotgun sequence.
ACCESSION   DAOQMB010000104 DAOQMB010000000
VERSION     DAOQMB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5521_length_6133_cov_15.579631"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3323
                     /gene="purL"
                     /locus_tag="PLY05_05140"
                     /gene_synonym="purI"
     CDS             <1..>3323
                     /gene="purL"
                     /locus_tag="PLY05_05140"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HNC02723.1"
                     /translation="QQQLHELLHYGEEFNAEGFGEGEFDAVFVITPRIGTISPWSSKA
                     TDIARNSGLDKVQRVERVTVYWLQTTSELSVEQRQAVAAKLHDRMTQSVLASVEEAEV
                     LFAEHEPQPLQKIDILGGGRDALKQANQEFGFALSPDEIDYLVESFQQLGRNPHDVEL
                     MMFAQANSEHCRHKIFNAEWTIDGEKADKSLFGMIKNTYNHAPANILSAYKDNASVIV
                     GHQADRFFPNPSSKTYGYSNEPVHILMKVETHNHPTAISPFAGASTGSGGEIRDEGAT
                     GRGGKPKAGLAGFTVSNLQIPNFVQPWEVAYGKPNRIVSALDIMIDGPLGGAAFNNEF
                     GRPNLCGYFRSFELAVNGVNGSEVRGYHKPIMIAGGLGNIREEHVQKNAIQAGDCLIV
                     LGGPALLIGLGGGAASSMASGASSENLDFASVQRDNPEMERRCQEVIDVCWAMGDNNP
                     LVSVHDVGAGGLSNAMPELVHEWDLGATLELRKIPNLERGMSPREIWCNEAQERYVLA
                     VRPERLAEFEAICQRERCPFAVLGQAEKVQHLTVSDSHFANKAVDMPMQVLLGKPPRM
                     SRQFNSQHIEQASFDSSTIDLTEATKRVLQVPTVASKSFLITIGDRSVTGLVAREQMV
                     GRYQVPVADCAVTASGFHAFTGEAMAMGERTPLALIDAGASARMAVGESITNIASAKI
                     AQLSDIKLSANWMAAAGYGIEDQNLFNAVKAVGMELCPALGIAIPVGKDSLSMRTVWK
                     DGEENKAVTAPLSLIVSSFAPVTDIRQTITPELKTDADSVLILIDLGQGKNRLGGSVL
                     AQAYSQVGNVAPDLDSPNLLIDFFNGIQALNGKLLAYHDRSDGGLVATLAEMAFAARV
                     GLDVDLSALANNKQQLAAVLFNEELGAVIQVAKADVDAVTAAFANTSLANAVSVLGGI
                     NLTQDIRFNYQGEIVLQGTRAQWQQDWAETSYRIQAIRDNADCAAQEFALIADDTQAG
                     LIAKLSYDQDLDIALPFINTGVRPRVAILREQGVNGHIEMAAAFDKAGFTSVDVHMSD
                     ILTGRISLRDFVGLVACGGFSYGDVLGAGGGWAKSVLFNSRARDEFTAFFQRPETFSL
                     GVCNGCQMLSQLKDLIPHA"
     assembly_gap    3324..3423
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3591..4085)
                     /locus_tag="PLY05_05145"
     CDS             complement(3591..4085)
                     /locus_tag="PLY05_05145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC02724.1"
                     /translation="MFSPPQSLQKIHLVNAFNCDKASLNDWLKRYSLQAQQSGSAKTF
                     VVCDAQKVAAYFSLTLGQIDTFDAPDRVRQGMGQYPITVIILARLAVDRQYKGLGLGT
                     GLLKEAIKKALIIAEHAGVRALITHPLDEEAKTFYLRFGFVPSPTSQGLLVLLLKDAK
                     RVVT"
     gene            complement(4085..4384)
                     /locus_tag="PLY05_05150"
     CDS             complement(4085..4384)
                     /locus_tag="PLY05_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNC02725.1"
                     /translation="MSSIHQSTSHMRSSRLGLRATPVQEQLLKEAALLRHQSLTDFIL
                     DSACLAAEHTLLNQRLFMVSHQEYQNLLNLLDDDTPTENTGLADLFSRQAPWEQT"
     gene            4535..5851
                     /gene="yjjJ"
                     /locus_tag="PLY05_05155"
     CDS             4535..5851
                     /gene="yjjJ"
                     /locus_tag="PLY05_05155"
                     /inference="COORDINATES: protein motif:HMM:NF007297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HNC02726.1"
                     /translation="MQPTLLKLLAKQPQSAKELTQQLNISQPTLSRLVKQQPAIIKIG
                     KARATQYALQRPIRDIGSQWPVYRVNEQANISLAGQLIAIYPHSFVWHDALTQNDTLY
                     ESLPWFLWDIRPQGFLGREFSAIHQQLGLNPRLQDWQDDDVLTFLLKFGFVQPSHFLV
                     GEESCLSFLRLQQDVLQPDDDDGWLKTRASYGQEACKVIRHDIARSSAAGEQPKFGLF
                     VLESQQTFELLVKFSPPLATVNGQRWSDLLIAEHLALQTLQHFGIAAAQSQIVQTAGR
                     TFLEVLRFDRTTSFGRKGMVSLEAVSNEFIGHNKAWDEQAKSLLALNKINVDDYQNIL
                     KVFSFGRLIANEDMHNGNLSFFLAADFSLSLAPVYDMLPMRFAPTIHGEVVEREIKPV
                     QASALSQHVWSEVCVWATYYWQQVAHHPQISDEFRAIAEQYGRMMG"
     gene            complement(5948..>6133)
                     /locus_tag="PLY05_05160"
     CDS             complement(5948..>6133)
                     /locus_tag="PLY05_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02727.1"
                     /translation="FPEVALWTADAKNKRSPFVCIEPWFGHADLANGSPEFINKRGII
                     QLASQERFEAEYQILFF"
BASE COUNT         1673 a         1210 c         1421 g         1729 t
ORIGIN      
        1 caacaacaat tacatgaatt attgcattat ggtgaagagt ttaatgccga gggttttggc
       61 gaaggtgaat ttgatgcggt gtttgtgatt acgccacgca ttggcacgat ttctccgtgg
      121 tcatctaaag cgactgacat agcgcgtaac agtggcttag ataaagtaca acgtgttgag
      181 cgtgttaccg tctattggtt acaaaccacc agtgaattaa gcgtagagca acgccaagcc
      241 gttgccgcta aattacatga ccgcatgacc caaagcgtat tagccagtgt cgaagaggca
      301 gaagtgttat ttgccgaaca tgagccacag cctttacaaa aaattgatat tttaggcggt
      361 ggtcgtgatg ccttaaaaca agccaaccaa gaatttggtt ttgctttatc acctgatgaa
      421 attgattatt tagttgagag ctttcaacaa ttaggccgta atccacacga tgttgaattg
      481 atgatgtttg ctcaagccaa ctctgagcat tgtcgtcata agatttttaa tgctgaatgg
      541 acaattgacg gcgaaaaagc cgataagtct ttatttggta tgattaaaaa tacctacaat
      601 catgcaccag ccaatatttt gtctgcctat aaagataatg cttcagtgat tgttggtcac
      661 caagctgacc gctttttccc aaatccaagc agcaaaacct atggttatag caatgaacct
      721 gttcatattt taatgaaggt tgaaacccac aaccacccaa ccgcgatttc tccgtttgca
      781 ggggcatcga caggttctgg tggtgaaatt cgtgacgaag gcgcaacagg tcgtggtggc
      841 aaacccaaag caggtttagc aggctttacc gtatccaact tacaaatccc caattttgtg
      901 caaccttggg aagtggctta tggcaagccc aatcgtattg tgtctgcttt agacattatg
      961 attgacgggc ctttgggcgg tgcagccttt aataacgaat ttggtcgccc caatttatgt
     1021 ggttatttcc gtagctttga attagcggta aatggtgtta atggcagtga agtccgtggt
     1081 tatcataaac cgattatgat tgcaggcggt ttgggtaata ttcgtgaaga gcatgtgcaa
     1141 aaaaatgcca ttcaagcagg ggattgcctg attgtgttag gtggcccagc gttgttaatt
     1201 ggtttgggtg gcggtgctgc gtcgagtatg gccagtggtg caagctctga aaacttagat
     1261 tttgcctcgg tacaacgtga taaccccgaa atggaacgcc gttgccaaga agtgattgat
     1321 gtgtgttggg caatgggtga caacaatcct ttagtctctg tacatgatgt gggcgcgggt
     1381 ggcttgtcga atgcgatgcc tgaattagtt cacgagtggg atttaggcgc aaccttagag
     1441 ttaagaaaga ttcctaattt agaacgtggt atgagcccac gcgaaatttg gtgtaacgaa
     1501 gcgcaagagc gttatgtgtt ggcggttcgt ccagaacgtt tagccgaatt tgaagccatt
     1561 tgtcagcgtg agcgttgtcc atttgcggtg ttaggtcaag ctgagaaagt gcagcaccta
     1621 acagtcagtg acagtcattt tgccaacaaa gcagtcgata tgcccatgca agtgttgttg
     1681 ggcaaaccac caagaatgtc gcgtcaattt aacagccaac acattgaaca agccagtttt
     1741 gatagcagca ctattgattt aaccgaagcc acaaaacggg tattacaagt gccaacggtt
     1801 gcttctaaat cattcttaat taccattggc gaccgctcgg tgacaggttt agtagcccgt
     1861 gaacaaatgg tcggtcgtta tcaagtgcct gttgcggatt gcgcggtgac tgccagtggt
     1921 tttcatgcgt ttacaggcga agcgatggca atgggtgagc gtactccctt agccttgatt
     1981 gatgcaggtg cttctgcacg tatggcggtt ggcgagtcga ttaccaacat tgcttctgcc
     2041 aaaattgctc agttaagcga tattaaatta tccgcaaact ggatggctgc ggcaggttat
     2101 ggcattgaag accaaaactt gtttaatgcg gtaaaagccg tgggcatgga attgtgtcct
     2161 gccttgggta ttgccattcc tgtgggtaaa gactcgttgt cgatgcgtac cgtatggaaa
     2221 gacggtgaag aaaacaaagc cgtgactgca cctttgtcct tgattgtgtc ttcgtttgca
     2281 cctgtcaccg atattcgtca aaccatcacc cctgaattaa aaacagacgc agacagcgta
     2341 ttgatattga ttgatttggg tcaaggcaaa aatcgcctag gtggttcagt cttagcacaa
     2401 gcctatagcc aagtggggaa tgttgcgccc gatttagata gccccaactt attaattgat
     2461 ttctttaatg gcatccaagc actcaacggc aagttattag cttaccatga ccgtagtgat
     2521 ggtggtttag tggcaacttt agcagaaatg gcgtttgccg cacgtgtcgg tttagatgtc
     2581 gatttatctg ctttagcaaa caacaagcaa caattagctg ccgtgttgtt taacgaagaa
     2641 ttgggtgcag tgattcaagt ggccaaagcc gatgttgatg ccgtgacagc cgcatttgcc
     2701 aacaccagtt tagccaatgc tgtatcagta ttaggcggca ttaacctgac tcaagacatt
     2761 cgctttaatt atcaaggcga aatcgtgtta caaggcacac gcgcacaatg gcaacaagat
     2821 tgggcagaaa ccagttatcg tattcaagcc attcgtgaca atgccgactg tgcagcccaa
     2881 gaatttgcgt taattgctga tgatacgcaa gctggtttga ttgctaagtt aagttatgac
     2941 caagatttag acattgcctt gccatttatt aacacaggtg tacgtccacg tgtggcgatt
     3001 ttgcgtgaac aaggtgtcaa tggtcatatc gaaatggcgg ctgcttttga taaagcaggt
     3061 tttaccagtg ttgacgttca catgagcgat attttaacag gccgtattag cttacgtgac
     3121 tttgttggtt tagtggcttg tggtggattc tcttatggtg atgtgttagg tgctggtggc
     3181 ggttgggcaa aatctgtatt gtttaacagc cgtgcgcgtg atgaatttac ggcgtttttc
     3241 caacgtccag aaacctttag cttaggggtg tgtaacggtt gtcaaatgtt gtcacagctc
     3301 aaagatttga ttcctcatgc tgannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnntgatggt cgtgcaacga ttttaatgcc ccatcctgaa cgtgtgttcc gtggcgtgca
     3481 aaactcttgg cagccaaaag attggcaaca agatggcgcg tggatgcgta tgtttagaaa
     3541 tgcacgggtg tttgtgggtt aagataaaag ggcgcagatt gcgccctttg ttatgtcaca
     3601 acacgttttg catctttcaa caacaaaact aacaaaccct gacttgtagg agatggcaca
     3661 aatccaaaac gtaaataaaa tgttttagct tcttcgtcta aggggtgtgt aatcagtgcg
     3721 cgtacgcctg catgttcggc aataattaac gcttttttta tagcttcttt taataaacca
     3781 gtccctaacc ccaaaccttt atattgtcta tcaaccgcca aacgcgccaa aataatcaca
     3841 gtaatagggt attgccccat gccttgacgt actctatctg gcgcatcaaa ggtatcaatt
     3901 tgacccaatg ttaaactaaa gtaagcggca actttttgtg catcacaaac cacaaaagtt
     3961 ttagccgagc cactttgttg tgcttgtaaa ctgtagcgtt taagccagtc atttagagag
     4021 gctttgtcac aattaaacgc atttactagg tgtatttttt gtagactttg tggcgggctg
     4081 aacattaagt ttgttcccaa ggagcttggc gcgaaaataa atcagcaagc ccagtgttct
     4141 ctgttggggt atcgtcatca agtaagttca gtaagttttg atattcttgg tgagaaacca
     4201 tgaacaagcg ttgattaagc aaggtgtgtt cggcagctaa acaagcacta tctaaaataa
     4261 aatcggtgag tgattgatga cgtaacaaag cggcttcttt taataattgc tcttgtacag
     4321 gagttgcgcg taaacctaaa cgagatgagc gcatatgtga ggtagattgg tgtatactag
     4381 acatggtatt cgcctctttc ttaagcggtg tttgtacaat gttagcacaa aaatagttat
     4441 aagtgtacca ttaaaaaatc tattcactcc tctcacataa atcctaataa atatgatata
     4501 agagaataaa aataacccaa aaaatctatt catcatgcaa ccaactttgc tcaaactatt
     4561 agccaaacag ccgcaatcgg ccaaagagct aacccaacaa ctcaatatct cgcagccgac
     4621 tttatcgcgt ttggtcaaac agcagcctgc cattattaaa attggcaaag cccgcgctac
     4681 gcaatatgcc ctgcaacgtc cgattcgtga tattggtagt caatggcctg tttaccgagt
     4741 gaatgaacaa gcaaacatca gtttggcagg gcagctaatc gccatttatc cacatagttt
     4801 tgtttggcac gatgcactga cccaaaacga tacgctttac gaaagtttgc cgtggttttt
     4861 gtgggatatt cggccacaag gctttttggg gcgtgaattt agtgccattc atcaacagct
     4921 tggccttaat ccacgtttac aagattggca agatgacgat gtattaacct ttttattaaa
     4981 gtttggtttt gttcagccga gtcatttttt agtgggcgaa gaaagttgtt tgtccttttt
     5041 gcgtttacaa caagatgtct tgcaacccga tgatgatgat ggttggctaa aaacacgcgc
     5101 ctcgtatggc caagaagcgt gtaaagtgat taggcatgat attgcccgtt cttctgcggc
     5161 aggggaacaa ccaaaatttg gtttatttgt cttagaaagc caacaaacct ttgagctatt
     5221 agtaaaattt agtccacctc ttgccacagt caatggtcag cgttggtcag atttattaat
     5281 tgctgagcat ttagccctac aaactttaca gcattttggc atcgctgcag cacaaagtca
     5341 gattgtgcaa acagcagggc gtactttttt ggaggtgctg cgttttgatc gaacaacctc
     5401 ttttggacgt aaaggcatgg tgtctttaga ggcagtaagc aatgagttta ttggtcataa
     5461 caaagcgtgg gacgaacaag ccaaatcgtt attggcctta aataagatta atgttgatga
     5521 ttatcagaac atcttaaaag tattcagttt tggccgttta attgctaatg aagatatgca
     5581 caacggcaat ttaagtttct ttttagcagc cgactttagt ttaagccttg cacctgttta
     5641 cgatatgttg ccaatgcgtt ttgcgccaac cattcacggc gaggtagttg agcgcgagat
     5701 aaaacctgtt caagcgagtg cattaagcca acatgtgtgg tctgaggtgt gtgtttgggc
     5761 aacatattat tggcagcaag tcgcgcatca tccgcagatt agtgatgaat ttagagctat
     5821 tgctgagcaa tatggccgta tgatggggtg aagaactaaa cccatctaaa taaaagagaa
     5881 aattaccctt cgactacgct cagtgtacat tgtgtagtag ttgagtggta gctgagcgta
     5941 gtcgaagcta aaaaaacaat atctgatatt cggcctcaaa acgctcttga ctcgctaact
     6001 gaataatacc gcgtttattg ataaactcag gcgagccatt cgctaaatcg gcatgaccaa
     6061 accacggctc aatacacaca aaaggcgaac gtttattttt ggcatccgcc gtccacaacg
     6121 ccacttcagg aaa
//
LOCUS       DAOQMB010000105         5991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5720_length_5991_cov_18.288410, whole genome shotgun sequence.
ACCESSION   DAOQMB010000105 DAOQMB010000000
VERSION     DAOQMB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5991
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5720_length_5991_cov_18.288410"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLY05_05165"
     CDS             <1..789
                     /locus_tag="PLY05_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNC02728.1"
                     /translation="TKMPEKNADSILVHPDVRRMLLKIRANNEAGRAFALYTAQQLDV
                     TKFSDDPAARKLAADRVALLTPVVKAYLTDKGLENCIEGQQVLGGHGFIREWGLEQWV
                     RDTRIAQIYEGTNGVQAHDLMGRKVAKNNGAFVAPLIAEMREFNATLAGNANLAFIHD
                     ALEQAAQDIESATQFVIEQSQQNPDQINAAAVDYLHAFGLLNFAYMFAKIAKAADGKD
                     DTFYADKLRLAKFFVTRMLPELKSRVASLQAGSDVMMDFDMAYF"
     gene            907..1923
                     /locus_tag="PLY05_05170"
     CDS             907..1923
                     /locus_tag="PLY05_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02729.1"
                     /translation="MNRYLFLLSSLSVLISTTVYGEAEIAPQQLLPADVLIEAINQNF
                     NQDTCIERDNEGRFWGWLDNQHCFLSSRATQTAQWLDGLFGQDHSENEYQAKSRIRIV
                     NDFSWLESSGLSANMRIRASLKLPNAKRRFHLVISDEEDTLNAERKTPVKDDESFKTS
                     AAIRWIPNIASRVRYSFDIGAHASDIFTRLRVQRAWRVSDDSLLRFRQSIRYGLKTET
                     KAVTELEAERLLNESTVLRIGSSLQYWQNEPESVGLRWSQSNTILHRISRNRSIAYGF
                     TVDGVQQPNWTVANDRLFFLYRQSIWRSWLYYELEPQLVRDWTTDKDIKSLFVLRLEA
                     NLGY"
     gene            complement(1932..4031)
                     /locus_tag="PLY05_05175"
     CDS             complement(1932..4031)
                     /locus_tag="PLY05_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC02730.1"
                     /translation="MRYPATLSWRHLGFVGSQLILLTSLIFPSESLLRWFLPSLCLLP
                     LLALSEQMRFLCQPTEQDQIYMRWYQYTVAASLVLNLVLPNLAAFSLSILLSITTFLI
                     VAWVIHHKTLYDRRHMIALLVCTLFISINHSFILILGYKGNFSLLAVLIITLCICASL
                     ISNLFGQESFRHFVEIEEKSPDEPSSPPPRDINNQQLSELSHDMRTPLSGILGMSELL
                     LETSLNMTQRDYVQTIQSSGNTLLHLINDRLDNPQAAEQELIILEEAFDVTELLHYCL
                     DMFKTQAEDKNIELISYLDSSLPKQILGDAVRLRQILSRLLHNAIRFTQSGEVIISIK
                     YVTLGKQLGIQFSVQDTGVGMSESDINAALQAKPIQQSRHTWHIAPELNACAQILQMM
                     GGELHIDSIPHQGTTVSFNLPLKSPNTGFNDSTTEFIASFAGKRLLVVDDNLTVTKLL
                     AEQATQWGMRVSYATSGAEALAVARNAANLGQAFDVIIMDYQMPGMSGLQLAVRLKED
                     TLITNDVILLMLTGLRQNNIQTLARNAGIHRVITKPITNKSLRQIISQELKRQQEIQQ
                     NLKQPERQLDHLHVLIAEDNQLGQKVIHGMMQKLGVNHTIVNNGQEAVQAVMNNKFDI
                     ILMDCDMPIMDGYTATQEIRRWEKKGLRSATPILALTAHILEEHKQKALNAGMNKHLS
                     KPVELGELQNALLEFCS"
     gene            4265..>5991
                     /locus_tag="PLY05_05180"
     CDS             4265..>5991
                     /locus_tag="PLY05_05180"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNC02731.1"
                     /translation="MLEKLKIQLKHFPIWMGLVILSLFVVLELIAINKPHSVADNWLK
                     RVDTLVYDWRFQGFMPKREETTKIVIIDVDERSLKQEGRWPWSREKLAKLVEALQRQQ
                     VKLIGFDVVFSEEERNAVQQVLAHSGLSDQAKQELQPLIPVLDGDKKFAQAITQKTVL
                     GFFLHGAGGINSGVLPTPLLTVEPRDLDKITINFMPDYTGNLAVFANAALGEGFITTL
                     PDVDGVMRRSPLVLRYENGLYSSLSLELARIYLNQSSIQLQMVKSGGQLRLESINLGN
                     HKIYTDESGMALIPYKGKGKSYTYISATDILNNPNNMPVLKDAIVLVGTSALGLTDLR
                     TTPLQTGYPGVEIHANLLDAIIQSVGDQNHMYYRPDWEPGITFLILLLSGLAFVFILP
                     MLEPAYMLAVAGAWLTLLVIVNLVSWKVSHLDFPLAILVISTFSIAILNIGYGFLRTN
                     NQKREMKMLFGQYVPPAHVEHMLENHHLAGLEGESRHMTVLFSDIRNFTTISEGLKAT
                     KLKQMLNEFFTPITGIIFQHNGTIDKYVGDMVMAFWNAPLLDANHAEHAIDAALSMLI
                     KVEELKPIFV"
BASE COUNT         1821 a         1024 c         1242 g         1904 t
ORIGIN      
        1 acaaaaatgc cagaaaaaaa tgctgacagc attttagtac atcctgacgt tcgtcgtatg
       61 ttattaaaaa ttcgtgctaa caacgaagca ggtcgtgctt ttgctttata cactgcgcaa
      121 caattagacg tgactaaatt tagtgatgac ccagcagctc gtaaattagc cgctgatcgt
      181 gttgctttat taacacctgt tgttaaggcg tatttgaccg ataaaggttt agaaaactgt
      241 atcgaaggtc aacaagtatt gggtggtcat ggctttattc gtgaatgggg cttagagcaa
      301 tgggtgcgtg atacccgtat tgctcaaatt tatgaaggga ctaacggcgt acaagcccat
      361 gacttaatgg gtcgtaaagt agccaaaaat aacggtgctt ttgttgcacc attaattgct
      421 gaaatgcgcg aatttaatgc aaccttagct ggtaatgcta acttggcttt tattcatgat
      481 gctttagagc aagccgctca agacattgaa agtgcgacac aatttgtgat tgagcaatct
      541 cagcaaaatc ctgaccaaat caatgctgct gcggtagatt atttacacgc ctttggtttg
      601 ttaaattttg cttatatgtt tgccaaaatt gctaaggcgg cagatggtaa agacgatact
      661 ttctatgccg ataaattacg tttggctaaa ttctttgtca ctcgtatgtt gccagagtta
      721 aagtcgcgtg ttgctagctt acaagcaggt tctgacgtga tgatggattt tgacatggct
      781 tatttctaag tcggattaac gtctgataca aaaaggagtg ctagtcactc cttttttatt
      841 gcctaacgat caataagtta tttacactaa aaccacaatc aaaacgtcaa cagaccctga
      901 tattttatga atcgctatct ttttttgcta tctagtctta gtgttttaat aagtactact
      961 gtttatggtg aagctgaaat tgctccacaa caattgttgc cagcagatgt attaattgaa
     1021 gcaattaacc aaaattttaa tcaagatact tgtattgagc gtgataatga agggcgtttt
     1081 tggggatggt tagataatca acattgtttt ttatcgagtc gggcgacaca aacagcacaa
     1141 tggttagatg gcttatttgg tcaagatcat agcgaaaatg aatatcaggc caaaagtcgg
     1201 attcgtattg tgaatgattt ttcgtggtta gaaagtagtg gtttgagtgc taatatgcgg
     1261 attagagctt ctttaaaact acctaatgcc aaacgacgtt ttcatttagt gattagtgat
     1321 gaagaggata ctctcaacgc tgaacgtaaa acgcctgtta aagatgacga aagttttaaa
     1381 accagtgctg cgattcgttg gattcctaat attgcctcgc gtgtgcgtta tagctttgat
     1441 attggtgcgc atgccagtga catttttacc cgtttgagag tgcagcgagc atggcgagtc
     1501 agcgatgaca gtttattacg ttttcggcaa tccattcgtt atggccttaa aacagaaaca
     1561 aaggcagtga cggagttaga agcagagcga ctgttaaatg aatctactgt gctaagaata
     1621 ggatcatctt tgcaatattg gcaaaatgag ccagagtcag ttggcttgcg ttggtcacaa
     1681 agtaatacga ttttacatcg tatcagccgt aatcgtagta tcgcttatgg ttttacggtg
     1741 gatggtgtgc aacaaccaaa ttggactgtt gctaatgatc ggctgttttt tttatatcgg
     1801 cagtccattt ggcggtcttg gttgtactat gaattggagc cacaattagt tcgagattgg
     1861 acaacagata aagacataaa atctttattt gtactaagat tagaagcaaa cttgggatat
     1921 taggtcagcc tttacgaaca aaattctaat aatgcatttt gtagttcacc caattcaaca
     1981 ggtttactaa ggtgtttatt catgcctgca tttaaggctt tttgtttgtg ttcttctaag
     2041 atatgagccg ttaaagccaa aataggtgtg gcagagcgta accccttttt ttcccatcgt
     2101 ctaatttctt gggtagcggt ataaccatcc ataattggca tatcacaatc cattaaaata
     2161 atatcaaatt tattattcat cactgcttgc acagcttctt ggccgttgtt aacaatcgta
     2221 tgattgactc caagcttctg catcatgcca tgaatcactt tttgtcccaa ttgattatct
     2281 tcagcaatta aaacgtgtaa atgatccaat tgtcgctcag gttgtttaag attttgttga
     2341 atttcttgtt ggcgtttaag ttcttgacta atgatttgtc tgagggattt attggtaatc
     2401 ggtttggtaa taacgcgatg aataccggca ttgcgtgcta atgtttgaat attgttttgt
     2461 cgtaggcctg ttaacattaa taaaatcaca tcattagtga tgagtgtatc ttctttgaga
     2521 cggactgcca gttgcaaacc actcatacca ggcatttggt agtccataat aattacatca
     2581 aatgcttgac ctaaattggc tgcattacgt gctacagcaa gtgcttccgc accacttgtt
     2641 gcataactga cgcgcattcc ccattgagtt gcttgttcgg caagtaattt ggtcacagtt
     2701 agattgtcgt caacaaccaa taaccttttg cctgcaaaag aggcaataaa ttcggtggta
     2761 gagtcgttaa aaccagtatt aggtgattta agtggcaagt taaaactgac tgttgtgcct
     2821 tgatgaggga tgctatcaat atgtaattca ccacccatca tttgcaaaat ttgtgcacag
     2881 gcattaagtt cgggggcaat atgccatgta tgtctacttt gttgtatagg cttggcttgt
     2941 agagcagcgt ttatgtcaga ctcgctcata ccaacccctg tatcttgtac actgaattgt
     3001 atgcctagtt gtttgcctaa tgtgacgtat ttgatactaa taatgacttc accagattga
     3061 gtaaatctaa tggcattatg tagcaagcga gacaaaattt gccgtaaacg aacagcatca
     3121 cccaagattt gcttgggtaa agaagaatct aaataagaga ttaattctat atttttgtct
     3181 tcagcctgtg tcttaaacat gtctaagcag tagtgtaata gctctgtaac atcaaaggct
     3241 tcttctaaaa taatcagctc ttgttctgcg gcttgaggat tgtctagtcg atcattaatc
     3301 aagtgcaaga gagtattgcc tgatgattga atcgtttgga cataatcccg ttgagtcata
     3361 ttaagtgagg tttctaacaa taattcggac atccctaaaa tcccactgag aggtgtgcgc
     3421 atatcgtggc ttaattcact gagttgttga ttgtttatgt cacgtggggg aggagaggac
     3481 ggctcgtctg gagatttttc ttcaatttca acaaaatgtc taaaactttc ttgtccaaac
     3541 agattactaa ttaaagaggc acaaatacag agggtgataa ttaacacagc caataaagaa
     3601 aagttacctt tgtagcccaa aattaaaata aaactatgat tgatgctaat aaatagtgta
     3661 cataccaata acgctatcat atggcgacga tcatagagtg ttttatgatg aatcacccaa
     3721 gcaacaatta aaaaagtagt aatacttaat aaaatagaga gactaaatgc ggcaagatta
     3781 ggcaaaacta aatttagaac aagactagct gcaacagtgt attgatacca acgcatataa
     3841 atttgatctt gttctgtggg ttggcacaaa aagcgcattt gttcgctaag ggcaagtaac
     3901 ggtaaaaggc ataatgaggg taaaaaccaa cgtaataatg attcagaggg aaaaatcagc
     3961 gacgttaata aaatcaactg gctaccgaca aagcctagat gtcgccagct cagtgttgca
     4021 ggatagcgca taatccaagg tagtgatata aaggctaatc caaagcccaa agctattacg
     4081 aagagccaag ctgattctaa caccatacag accctatcct cattaaaatt ggcaatacca
     4141 cggccatgca cctaaattat ctataggcta acatatattg ccaataatgg gttaatatca
     4201 tcaaaagctt ggctatgttc aattttaaaa gtcatgagct gctacgatta gggaattaat
     4261 aattatgcta gaaaaattaa aaattcaatt aaagcatttt ccgatttgga tgggattagt
     4321 tattttatcg ctatttgtgg tgttggagtt aatagcgatt aataagcctc atagtgttgc
     4381 agataattgg ttaaagcgag ttgatacgct agtttatgat tggcgttttc aggggtttat
     4441 gcctaaacgc gaagaaacga ccaaaatcgt gattattgac gttgatgaac gcagtttaaa
     4501 gcaagaggga cgttggccgt ggtcgagaga gaaattagcc aaattagttg aagcattaca
     4561 acgtcaacaa gtaaagttaa ttggctttga cgtggtattt tcagaagaag aaagaaatgc
     4621 agtgcaacag gtattagcac attctggatt aagtgaccaa gctaaacaag aattacaacc
     4681 tttaattcca gtattagatg gtgataaaaa atttgctcaa gcgattactc aaaaaacggt
     4741 attaggcttc tttttacatg gtgcaggcgg cattaatagt ggggtattgc caacaccatt
     4801 attaaccgtt gaaccccgtg accttgataa aattactatc aactttatgc ctgattacac
     4861 gggtaattta gccgtatttg ccaatgcagc attgggtgaa ggttttatta ccacattgcc
     4921 tgatgttgat ggagtcatgc gtcgctcacc tttagtgtta cgttatgaaa atggtttata
     4981 tagctctttg tctttggaat tggctcggat ttacttaaat caatcgtcta ttcaattaca
     5041 aatggttaaa agtggtggtc aattacgttt agaatcaatt aatttaggta atcataaaat
     5101 ttataccgat gaatcgggta tggcattaat tccttataag ggtaaaggta aaagttatac
     5161 ctatatttca gcgactgata ttttaaacaa tcctaataat atgccagtct taaaagatgc
     5221 tattgtttta gtaggtacat cagcacttgg tttaacggat ttaagaacca cccccttaca
     5281 aactggctat cctggtgtag aaattcacgc caatctatta gatgcaatta ttcaaagtgt
     5341 gggtgatcaa aaccacatgt attatcgccc tgattgggaa ccaggtataa cctttttaat
     5401 tttgttgtta agtggtttgg catttgtttt tattttgcca atgcttgaac cagcctatat
     5461 gttggccgtt gcaggtgcat ggttaacttt attagttatt gttaacttgg tgtcatggaa
     5521 agttagtcat ttagattttc ctttggctat tttggtcatt tctacgttca gtattgccat
     5581 tttaaatatt ggttatggct ttttgcgtac caataatcaa aaacgtgaaa tgaaaatgct
     5641 ctttggccaa tatgtgccac ctgcacacgt tgagcatatg ttggagaatc atcatttagc
     5701 aggtttagaa ggtgaatcac gacacatgac ggtgttgttt tcggatattc gtaatttcac
     5761 caccatttct gaaggcttaa aggcaaccaa acttaagcag atgttaaatg agttttttac
     5821 gccaattaca ggtattattt ttcaacataa tggcacaatt gataaatatg tcggtgacat
     5881 ggtgatggct ttttggaatg caccgttgtt agatgcaaac catgctgaac acgccattga
     5941 tgctgctttg tcgatgttaa ttaaagtaga agagttaaaa ccgatatttg t
//
LOCUS       DAOQMB010000106         5989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5728_length_5989_cov_31.693461, whole genome shotgun sequence.
ACCESSION   DAOQMB010000106 DAOQMB010000000
VERSION     DAOQMB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5728_length_5989_cov_31.693461"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(173..1258)
                     /locus_tag="PLY05_05185"
     CDS             complement(173..1258)
                     /locus_tag="PLY05_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC02732.1"
                     /translation="MTTLIMLPHLGDIESKAVLKKTAKAHQALAELKGVVGSIPNQAI
                     LLNTLPLQEAKDSSAIENIITTHDDLYQSDSLARQFISLAAKEVHNYATALRRGFEQV
                     QQTGLLTERHILEIQQCIEENDAGYRTQAGTVLKNQQTGEVVYEPPQHPDDIINYMRN
                     LEQFINDDQLCDWDDLVKMAVIHHQFESIHPFYDGNGRTGRIINILFLVKQNLLGLPV
                     LYLSRYINQHKSSYYSLLQTTRNTNDWEEWLLFMLEAVEQTSQQTTVLIKQIRDLMQH
                     HKIALRDKLPKIYSQDLINNLFCHPYTKIDFVANELQVSRQTAARYLDEIVSIGLLSK
                     HKIGKDNYYLNDDLFLLLSAVGQKNLA"
     gene            complement(1255..1590)
                     /locus_tag="PLY05_05190"
     CDS             complement(1255..1590)
                     /locus_tag="PLY05_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02733.1"
                     /translation="MDIKQLDKDLWEFADNLCANSKLTATEYAASVLGLVFLSHASSR
                     SHKLIQRIEQEAPTAQKMAHIVETVLQGLNALYLDVCSVYRIVSTKLHVFSDSQLDAS
                     GLRLKEMAL"
     gene            complement(1746..1985)
                     /locus_tag="PLY05_05195"
     CDS             complement(1746..1985)
                     /locus_tag="PLY05_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02734.1"
                     /translation="MFNLLVVLFMALGRKKTGTNNPLLAEVVTEAQKENLIPLHVQIP
                     ASLHQQTKMFAVQSGQSVKDIVIAALEYHLEQQSK"
     gene            complement(2012..2653)
                     /gene="parA"
                     /locus_tag="PLY05_05200"
     CDS             complement(2012..2653)
                     /gene="parA"
                     /locus_tag="PLY05_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007117279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HNC02735.1"
                     /translation="MYTIAFLSQKGGAGKTTLATCVARQLQLEGEDVILVDSDPQGSA
                     RDWRAASEKDSVVVVGVDRPTLDKDIKTLSNNGKRWVVVDGAPRSEQMTVSTIKAADI
                     VLIPVQPSPYDVWAAEDLVSIIRARQEVTDGKPKAAFLISRAIKRTELSKEVVGALED
                     FELPVFVSKTTQLVTYPKSASIGSTPMDTEPDGNATKEIRAIVAELRQMLQQK"
     gene            complement(2756..4069)
                     /locus_tag="PLY05_05205"
     CDS             complement(2756..4069)
                     /locus_tag="PLY05_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HNC02736.1"
                     /translation="MTKNNDIQSVVRKVTKRQIKTPQSLIHIKHTISLFQYKLWILLL
                     QEFKRQFDLNEPTDDRGFRYVSMSYIEQHIGYAPSRQDIIDDLRALRKEEICFNYFEK
                     DGNKTQYFSGFISEMKVTRLTIGFKFPSVIDDVMLGLDDAKAIFQMLNWDVFNHFSGK
                     YEAIIYKLCKDYLGVGRTPYMTVDEFREYMGIKPTEYSEFMKLNEWVIRKPTKSINTS
                     DISDITVEPIYSRNGRKVIGLHFLVTPKHQTSFPFIESHTNPAFAHAKVTIALSDQET
                     YLALFSSEQISLSIDRANQYCDELKKKEKTINYGAIYKTAITENWGQQIIEQRSINTS
                     EQLDVSNDTLKNLASQPQNTKKSDESDWSIIHQKFLIRFTALPEDEQHELIQGCIKAQ
                     KDVFKGVMRKNYEALDLKILEKPSFTTLLWPYLAELWQEPITKIL"
     gene            5006..5419
                     /locus_tag="PLY05_05210"
     CDS             5006..5419
                     /locus_tag="PLY05_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02737.1"
                     /translation="MSANRKSVFLSEESLSIVGPFTSGRLNSIIERYGYLLKTQDIAD
                     VFSPSQLALIYQVTKERATKERESWRQFHSITLRDLLNTVKTDDNCRNIDEMIDTINE
                     LKPLQWVALIDAIEMFWADERVRLFQAYQLRKKQP"
     gene            5504..5746
                     /locus_tag="PLY05_05215"
     CDS             5504..5746
                     /locus_tag="PLY05_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02738.1"
                     /translation="MNMPVLDIKSSLSTILQKLFTFTQDAIQQLCALCVYGTFFVCLV
                     ILGIATHTLMNQQHLHLVATIDGKEHIVIDLRPHSK"
BASE COUNT         1843 a         1126 c         1070 g         1950 t
ORIGIN      
        1 gttgatttgt tgatttgttg atttgttgat ttgttgattt gttgatttgt tgattaatat
       61 tatgatcctt aaaaaaataa catgttaaga aaagtgaagt tttcttaaca tgttctacaa
      121 ctcatgttaa agaaatggct gttttcttaa catgtttact ttttgtacaa cactaggcta
      181 gatttttttg accaacggca gataacaaca aaaataagtc gtcatttaaa taatagttgt
      241 ctttacctat tttatgctta gataacaaac caatagacac tatctcgtct aaatagcgtg
      301 cggcggtttg acgactgact tgtaattcat tagcgacaaa gtctattttt gtataaggat
      361 gacaaaacaa gttgttaatt aagtcttggc tgtaaatttt tggtaatttg tcacgtagag
      421 caattttgtg atgctgcatt aaatctctaa tttgtttaat caagactgtt gtttgttgtg
      481 aggtttgctc aaccgcttct agcataaata acagccattc ttcccaatcg ttggtgttac
      541 gtgtagtttg caacaagctg taatagctcg atttgtgttg attgatataa cgtgacaaat
      601 acaacacagg caaacctagc aaattttgct taaccagaaa caagatattg ataatacgac
      661 cagttcgtcc attaccatca taaaagggat gaatactttc aaactgatga tgaattaccg
      721 ccattttaac caagtcgtcc caatcacaaa gctggtcatc gttaataaac tgctctaggt
      781 tacgcatgta atttataatg tcgtcaggat gttgtggtgg ttcataaacc acttcgcccg
      841 tttgttggtt tttaaggacc gtgcctgctt gggtgcgata ccctgcatcg ttttcttcaa
      901 tgcactgttg aatctccaaa atatgccttt ctgtaagtag acccgtttgc tgtacttgtt
      961 caaagccacg gcgtaaagca gttgcgtagt tatgtacttc ttttgcagct aggctaataa
     1021 attgtcgtgc caaactatca ctttggtaaa gatcatcatg ggtggtaata atgttttcaa
     1081 tagccgagct gtctttggct tcttgtaatg ggagtgtatt aagcaaaatg gcctgattag
     1141 gaatactacc tactacccct tttaactctg caagggcttg atgggctttt gcagtttttt
     1201 taagaaccgc tttgctttct atatcgccca aatgaggcaa catgataagt gttgtcataa
     1261 ggccatttcc tttagtctta aaccagacgc atccaattga gaatcactaa atacatgtaa
     1321 cttagtcgat actattctat agactgagca cacatctaaa tacagtgcat ttaacccttg
     1381 aagcacggtt tcaactatgt gtgccatttt ttgcgcggtt ggggcttctt gttcaatacg
     1441 ctgtattaac ttgtgcgaac gcgacgaagc atgagacaaa aaaacaagtc ccaatacaga
     1501 cgctgcatat tctgttgctg ttagttttga gttagcacac aaattatcag caaactccca
     1561 aaggtctttg tctagttgct ttatatccaa aattacccca acatttcaac atttcaacat
     1621 ttcaacattt caacatttca acatttcaac atttcaatat tgatttaatt gtttatatca
     1681 aattaggtca atttaaaaat acgcatctgt ctgtatcaca gatgcgtttt tgagaaaggt
     1741 aaagattatt tagattgctg ctctaaatga tattccaacg ctgcaatgac tatgtcttta
     1801 accgattggc cagactgcac tgcgaacatt ttagtttgct gatgaaggct tgcaggtatt
     1861 tgcacatgca aaggtattaa gttttctttt tgcgcttcag tgactacctc agccaaaaga
     1921 ggattatttg tacctgtttt ttttcttcca agagccataa ataaaaccac caataaatta
     1981 aacatttgtt gctttcaaca aaacaacatt tctatttttg ttgtagcatc tgccgtaact
     2041 cagccacaat tgcacgaatc tctttagtag cattaccgtc tggctcggta tccatgggtg
     2101 ttgagccaat acttgccgac ttaggatagg tgacgagttg tgttgttttt gacacaaaaa
     2161 caggcagttc aaaatcttct aacgctccaa caacctcttt actaagctct gtacgcttaa
     2221 tggctctaga aattaaaaag gcagctttag gtttaccgtc tgtgacttct tgacgtgcac
     2281 gaataattga gactaggtct tcggcagccc aaacgtcata aggtgagggc tgaacaggaa
     2341 taagcactat atcggctgct ttaatggtag aaacggtcat ttgctcagat cgtggcgcac
     2401 catcaacgac tacccagcgt ttgccgttgt tggaaagcgt tttaatatcc ttgtcgagag
     2461 tcgggcgatc aacaccaact accacaacag agtccttttc agatgcggca cgccagtctc
     2521 tggcacttcc ttgtgggtct gagtcaacta agatgacatc ttcaccttct agttgcagtt
     2581 ggcgcgcaac gcacgttgct aaggttgttt ttcctgctcc acctttttgc gaaagaaatg
     2641 caatcgtgta cataagtttt tcatctatat ttaaaaacat ttgttgcaaa caacatttgt
     2701 ttaaatgttg tttgcaacaa atgtatagga tttatataaa ataacaagtt gtatgttata
     2761 agattttcgt aataggttct tgccataatt ctgctaaata tggccacaat aatgttgtaa
     2821 atgatggttt ttctaaaatt tttaaatcta aggcttcata gtttttgcgc atcacgcctt
     2881 taaatacgtc tttttgggct ttgatacaac cttgaatcag ttcatgttgc tcgtcttcag
     2941 gaagtgctgt aaaacgtatt aagaactttt gatgtattat tgaccagtca ctctcatcac
     3001 tttttttggt attttgtggt tgtgaagcca aattttttaa cgtgtcatta gatacatcta
     3061 actgttcact ggtatttatt gatcgttgtt caataatttg ttgcccccaa ttttcagtga
     3121 ttgcagtttt ataaatagct ccataattga ttgttttttc ttttttcttt agctcatcac
     3181 aatactgatt tgccctgtca atcgaaaggc taatttgctc cgaagaaaat aaagccaaat
     3241 aagtttcttg atctgataat gcaatcgtta cttttgcatg agcaaacgca ggatttgtgt
     3301 gtgactcaat aaaagggaaa gaggtttgat gcttaggagt caccagaaag tgaagcccta
     3361 taacttttcg accatttcgg ctgtatattg gctcaacggt aatatctgaa atatcacttg
     3421 tattgataga ttttgttggc tttctgataa cccattcgtt taatttcata aattcagagt
     3481 attccgtagg cttaatcccc atgtactctc taaattcatc cactgtcata tatggcgtac
     3541 gacctacgcc caaatagtct ttacacagct tataaataat ggcttcatat tttccagaaa
     3601 aatgattaaa aacatcccaa ttcagcattt gaaaaatggc tttagcatca tctaagccaa
     3661 gcataacatc atcaataaca ctgggaaatt taaatccaat cgtcaaacgt gttactttca
     3721 tttcagatat aaaccctgaa aagtattgag ttttattacc atctttttca aaatagttaa
     3781 aacaaatctc ttctttgcgc aatgcacgta agtcgtcaat gatatcttgt ctactaggcg
     3841 cataaccaat atgttgctcg atgtaactca tcgatacata acgaaagcct ctatcatcag
     3901 taggctcgtt aagatcaaat tgacgtttaa attcttgtaa taacaaaatc catagtttgt
     3961 actgaaacaa agaaatagtg tgtttgatat gaattaaact ttgtggggtt ttaatttgtc
     4021 ttttagttac tttgcgaacc acactttgaa tgtcattatt tttagtcatt actcttaaac
     4081 tactataaaa accactgaaa caaacatcat atcaccaatc atacagtttt taaaaaaata
     4141 tttctggatg atacataaaa taagtggggg ggttttctgg atgatagaaa ttaacgcatt
     4201 gtttttaatt tataattact ctgtaaaact tataaaaaaa accttttttc atgaaaaaag
     4261 aacaggtggg ggggttttct ggatgataca tgaaacaggt gggggggttt tctggatgat
     4321 agtggggggg ttttctggat gatagaaggc tgtatccctt tattaccaat agatacagcc
     4381 ctccctaaag tatttaaaac tactataaat acaaaagtag tagtaaaagg gagacttttt
     4441 taaaaatttg aatttgaatt ttttcaaaaa aagtataaaa atactcataa accctgtcca
     4501 cacagaaaac cctagttatc gtattagccg ttcaccctga gcttatcaaa aggcttttta
     4561 ccaatcgtta gacagactca gtatgagggt ttagtatgtt ttttaaacta gatgtggttt
     4621 aaactaaaat taaagactgc aattagtagt gattacccaa tcacaaatgc tcgaataata
     4681 aaaaacttaa acagtcgcca ttagcattta aatcacgtat aacgcacgat aacctaaaaa
     4741 ctatacaaac ccactaaaaa gcaaatgaac gcgctctagc gcgaaatttg gcatctttaa
     4801 atcgatgttt aaaaaaagta tggataaaat aagtttttct ataaaaaatc agctcgttcg
     4861 aggtgtcata gttcaataaa actggcagct ttgatcgctt gagttttatc ttatcaaccc
     4921 aaatcgtccg cgaaagtacg atatttttac gctaaaatac gctgttttta tctctctaat
     4981 acttattttt ctacaaggtt aggctatgtc cgccaatcgt aaatctgtat ttttatctga
     5041 ggaatcactc agtattgttg gcccctttac cagtggccga ttgaatagca tcattgaacg
     5101 atatggatac ttacttaaaa cacaggacat cgcggatgtc tttagtccaa gtcaattagc
     5161 actgatctat caagtgacca aagagcgagc gactaaagaa cgagagtcat ggcgacaatt
     5221 tcactcgata acgttaagag atttactcaa caccgttaaa acagacgaca actgtagaaa
     5281 catagacgag atgattgaca cgattaatga gctaaaacca ctacaatggg ttgccttaat
     5341 tgatgccatt gaaatgtttt gggcggatga acgcgttcgc ttatttcaag cttatcaatt
     5401 gcgtaaaaaa caaccataag accatcaaca aggcgttatt ttttgtgtag aatgggtttc
     5461 cctttaaacc aaaagtaccg aatgaggtac aaggtgaata cacatgaaca tgcccgtatt
     5521 ggatattaaa tccagcctat cgacgattct gcaaaaactc ttcacgttta cccaagacgc
     5581 catacaacag ctttgtgcct tatgcgttta tggtactttc tttgtctgcc tcgtgatcct
     5641 tggcattgcc acacatacgc tgatgaatca acaacatttg caccttgtgg ccaccattga
     5701 tggcaaagag catatcgtca tagacctacg accccacagt aaataaaccg ttttaaacgg
     5761 cgattgcatg aagaaaacgc ccttcaaatg aagggcgtta taacagatat ttctcaaaat
     5821 atctgggttt tgaccagtgc gaagctgatc aaaaggtcag tgcaaagctg accgtttcaa
     5881 ggcgcttgca ttcactgagt ttggataaac caatgctcaa tggtgcaagt agaagcgatt
     5941 tcatctgcct agctcgaatt acatcttagt cagactaaga acacactag
//
LOCUS       DAOQMB010000107         5983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5742_length_5983_cov_15.075405, whole genome shotgun sequence.
ACCESSION   DAOQMB010000107 DAOQMB010000000
VERSION     DAOQMB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5742_length_5983_cov_15.075405"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="PLY05_05220"
     CDS             complement(<1..448)
                     /locus_tag="PLY05_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC02739.1"
                     /translation="MFDLRHLKTLVAIRDTGSLADAAERLHLTQSALSHQLKDLEHSL
                     GLSLLLRKTRPPQFTKAGERLLALADEVLPIVKNTERELDKIRGGRSGRLILAIECHS
                     CFDWLMPTLNQFRDDWPEVDIDFQSGFQGDALQRLTSGELDLVITSN"
     gene            593..2875
                     /gene="metE"
                     /locus_tag="PLY05_05225"
     CDS             593..2875
                     /gene="metE"
                     /locus_tag="PLY05_05225"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HNC02740.1"
                     /translation="MKTHILGFSRIGAQRELKKALESYWSGQSHQHQLEEVGQSLRQR
                     HWQAQIDAGLDIITVGDFAFYDHVLNHSLRFGVIPKRFGDEQKADNLDLSFRLARGRA
                     PTGTDAAACEMTKWFDTNYHYLVPELEQDQRFSFADKRLLAEVKEAKQLNHPLKVVLI
                     GPLTYLWLSKTYGQAFDKLELLDNLLPVYAQIFNELMALGVNYIQIDEPILSLDLPNA
                     WQAAFERAYNRLQRRDLQLLLTTYFAPLNENLHLACHLPVAGLHIDVLRSGKEWQQVL
                     DILPDYKVLSLGVVDGRNIWRNSLAQSQQLLQQAQQKLGDRLWIASSCSLLHVPVDLA
                     NEQKLPTEIKDCFAFAIQKLAEISILKQAVLNPHSPLVEQLIAQNNAAYQLLNQSTHW
                     RQVAVQQRVQALTEQDSQRQSAFAERAIVQQQHLKLPLLATTTIGSFPQTSEIRRARA
                     AFKQGLLNEQAYEQAMQQEIQQAIHYQEQIDLDVLVHGEAERNDMVEYFGEQLQGFVF
                     SSNGWVQSYGSRCVKPPIIVGDISRPQAMTVKWASYAQQQSQKPVKGMLTGPVTMLAW
                     SFARTDISREQIALQLALAIRDEVSDLAQAGIHIIQIDEPAFREGLPLRQSEQANYWR
                     WAVRAFRISASGVDNHVQIHTHMCYSEFNDCIEHIAAMDADVITIETARSAMELLNAF
                     EAFDYPNAIGPGVYDIHSPQIPSVDAIKKLLNKAGQAIDKQRLWVNPDCGLKTRQWPE
                     VKQALTNMVQATKELREEWH"
     gene            complement(2944..5634)
                     /locus_tag="PLY05_05230"
     CDS             complement(2944..5634)
                     /locus_tag="PLY05_05230"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02741.1"
                     /translation="MKTVANLFATSYERLESDHQQDRQFIRLERLAPEEDSYCTYVKR
                     VDSLAQNEYWIAREKNILMLLKRVPHVARLRKEKEDEKTDDAYQTIKIKDAGISLAHW
                     LGTKPKLASNNHELKHPLVYVQSFLQFARYSLIALKEIHQAGVIHAQLRPDNICIPYK
                     PHPYTFDTPLHLNFNKLCLIDFMFAVSNTLKLSRPLPVHVNQKASTQSALMRHALLSD
                     QHQRHADLITRIDYSVDLYALGFILEQMFQQDLIYPAGLDAELSMEIHRFILELKSYD
                     NGIPESVKLRHLHLHPHGEYLAKIDHLLAISLQGHHPEQIELILDPAHFLEDDLVFNL
                     SPIIENQDNLINTTEPSDSVQPPLEPVATSALPLATSVEIKTMKETKPSKSLAQTQDH
                     IELSKTTVIALIVTLQVMYVLYADGKSMGLDVFASMGLVAVIGAAVMIAGKMFAPPQP
                     LPKRSVFDDLDDEDNSDNTSKTTATVTEVSSTPTSQAVDELDMIAASVAPAIANNVSA
                     ETVSEDKANVTVSNNETTSNTEIEIESVKKEEDNAPPPVMLFNKASEPSKNAKDDEAM
                     EINKWVVIAILIACQLAYVGLTTDFGGNSAPASAGDGTETAPVESTIEPTPEEIPPTD
                     TTATDDLLAETAPSSESSAANIELLSESSSVSTPQVTTPKTRSKEASSDSMLLSGSPT
                     AETKTKKEKTPTPAKTQESSPAVATTESTDAAKTETPAATPENKTIEVATPATAKDKE
                     MAQPKVEAKTEAKTPSTPKSLTRGLAEAQNAMGWHYYHGDGVPKNLEEAFKWFQKAAN
                     LGESSAQFNVGMMYQKGEGVKQDLAEAAKWYRKSAEQGKASAQLNLGMMYISGRGVRQ
                     NIDEGAKWLSKAADQGDTTAKANLAWLLQQGYIKEIPASE"
BASE COUNT         1709 a         1117 c         1283 g         1874 t
ORIGIN      
        1 ggtttgaggt gatgaccaaa tctaactcac ccgacgtaag ccgttgtaaa gcatccccct
       61 gaaagccgct ttgaaaatca atatccactt ctggccaatc atcacgaaat tgattgagcg
      121 ttggcattag ccaatcaaaa caactatggc actcaatggc caaaattaag cgtccagagc
      181 gaccaccacg aattttgtct agttcacgtt cggtattttt aacaatgggt aaaacttcat
      241 ctgccaaggc taataaacgt tcacctgctt tggtaaattg tggcggacgg gttttgcgta
      301 gcagcaaaga tagccccaag ctatgctcta aatctttcag ttgatgggat agggctgatt
      361 gagtgagatg caaacgttca gccgcatccg ccaaagagcc tgtatctctg atggcaacaa
      421 gagtttttag gtggcgtaaa tcaaacataa gcgataactg cagaagccat aattagctat
      481 ttatcatacg ttaatatgaa ttatattcaa ttttaatatt aaaaatatga gtttgtttca
      541 agttgttgtc tcatgaatac tatgcttatt attaatcaat cgagcattta acatgaaaac
      601 acatattttg ggtttttcgc gtattggcgc acagcgtgaa ttaaaaaaag cattagagag
      661 ttattggtct ggccaaagcc atcaacatca attggaagaa gttggacaaa gcttacgtca
      721 acgtcattgg caagcacaaa ttgacgcagg tttagatatt attaccgttg gcgattttgc
      781 tttttatgat catgtactta atcatagttt acgttttgga gtaattccta aacgttttgg
      841 cgatgaacaa aaagcggata atttagattt aagttttcgt ttggctcgtg gtcgtgcgcc
      901 aacaggtaca gatgcggcgg cttgtgaaat gaccaagtgg tttgatacca actatcatta
      961 tttagtgcct gagcttgaac aagatcaacg ttttagtttt gccgataaac gtttattggc
     1021 cgaagtgaaa gaggcaaaac aattaaatca tccgctaaaa gtggtgttaa ttggcccatt
     1081 aacttattta tggttaagta aaacttatgg tcaagccttt gataaattag agttattaga
     1141 taatttatta cctgtgtatg cccaaatttt taatgaatta atggcattag gcgttaacta
     1201 tattcaaatt gatgagccca ttttaagttt agatttgcca aacgcatggc aagcggcttt
     1261 tgagcgtgct tataaccgtt tacaacgtcg agatttacaa ttattactca ccacttattt
     1321 tgcaccttta aatgaaaatt tgcatttggc gtgtcattta cctgtcgcag gtttacatat
     1381 tgatgtcttg agaagtggta aagaatggca acaagtgctg gatattttac ccgattataa
     1441 agtgctatcg ttgggcgtgg ttgacggtcg taatatttgg cgtaattcac tagcgcaaag
     1501 tcaacaattg cttcagcaag cccaacaaaa gttaggtgat cgtttgtgga ttgctagcag
     1561 ttgttcattg ttgcatgtgc ctgttgattt agcgaatgaa caaaaattac caactgaaat
     1621 taaagactgt tttgcctttg ccatacaaaa attggcagaa attagcatct taaaacaagc
     1681 tgttttaaat ccgcattcac cattagtaga acagttaatt gctcaaaata acgcagcgta
     1741 tcaactgtta aatcaatcaa cacattggcg acaagttgcc gtgcagcaac gtgtgcaggc
     1801 attaacggag caagatagcc aacggcaaag cgcatttgcc gaacgagcta ttgttcagca
     1861 acaacattta aaattgccgc tattagccac aacaacgatt ggttcttttc cccaaacatc
     1921 agaaattcgc cgtgcgcgtg ctgcctttaa acaaggcttg ctcaatgaac aagcctatga
     1981 gcaagctatg cagcaagaaa ttcagcaagc tattcactat caagagcaaa ttgatttgga
     2041 tgtgttagtg catggtgaag ctgaacgtaa tgatatggtg gaatattttg gcgaacaatt
     2101 acaaggtttt gtttttagta gcaatggttg ggtgcaaagt tatggcagtc gttgtgtaaa
     2161 gccacccatt attgttggtg atatcagtcg tccacaagcg atgacggtca aatgggcgag
     2221 ttatgcccaa caacaaagtc aaaaacccgt caaaggcatg ttaacagggc cagtcaccat
     2281 gttggcatgg tcgtttgcac gaactgatat tagccgtgag caaattgcct tgcagttggc
     2341 cttagcgatt cgtgatgaag tgtctgattt ggcacaggca ggtattcata ttattcaaat
     2401 tgatgagcct gcatttagag aaggattacc gttacgtcaa tctgaacagg ccaattattg
     2461 gcgttgggca gtgagggctt ttagaatcag tgccagtggt gtggataacc atgtacaaat
     2521 tcatacccat atgtgttatt ccgagtttaa tgactgtatt gaacatatag cggcaatgga
     2581 tgctgatgtg attaccattg aaaccgcacg ttcggcaatg gagttattaa atgcttttga
     2641 agcctttgat tatcccaatg ccattgggcc tggtgtgtac gatattcatt caccccaaat
     2701 tcccagtgtt gacgccatca aaaagttgct caataaagca gggcaggcga ttgataaaca
     2761 acgattatgg gtgaatcctg attgtggttt aaaaaccaga caatggccag aagtgaagca
     2821 agctttaacg aatatggtgc aagcaacaaa agaattgaga gaggagtggc attaagggtg
     2881 gagggagata ttctccctca tctgcaaaga cagagccccc tttaataaag ggggctaggc
     2941 gggttattca ctagcaggga tttctttaat ataaccttgt tgtaataacc atgctaagtt
     3001 agctttagca gtagtatcgc cttggtcagc agctttgctt agccattttg cgccttcatc
     3061 aatattctga cgtacaccac gaccactaat gtacatcata cctaaattaa gttgtgcgct
     3121 ggctttacct tgctcagccg atttacgata ccatttggcc gcttcggcaa ggtcttgttt
     3181 tacaccttca cctttttgat acatcatgcc aacgttaaat tgcgcactag attcacctaa
     3241 attagccgct ttttgaaacc atttaaaggc ttcttctaag tttttaggca caccatcacc
     3301 atgataataa tgccaaccca tggcgttttg tgcttctgcc aatccacgtg ttaaagattt
     3361 tggtgttgag ggggttttag cctcagtttt ggcttctact tttggttgag ccatttcttt
     3421 atctttagct gttgcaggtg tggctacttc aatggttttg ttttcaggtg ttgctgcggg
     3481 tgtttctgtt tttgcagcgt ctgttgactc tgttgtagcg acagctgggc tagattcttg
     3541 tgtttttgca ggtgtaggtg ttttttcctt tttggttttg gtttctgctg ttggactacc
     3601 tgataacaac atactatcag agcttgcttc tttgctgcgt gttttgggtg tagtcacttg
     3661 tggagtgcta acagagctgc tttcactgag caattcaata ttagctgcag aagattcgct
     3721 actaggagca gtctcagcca ataaatcatc tgttgctgtg gtatcagttg gtggaatttc
     3781 ttcaggggta ggttcaatcg tagactcaac aggagctgtc tcggtaccgt ccccagcact
     3841 tgcaggtgcg ctattaccac caaaatcggt tgttaaccct acataagcta attgacaggc
     3901 aatcaaaatc gcaatgacca cccatttatt gatttccatc gcctcgtcat cttttgcatt
     3961 tttgcttggc tctgaggctt tgttaaaaag catgactggt ggaggggcat tgtcttcttc
     4021 tttttttaca gattctattt ctatctctgt atttgatgtt gtttcattat tgctaacagt
     4081 tacgttggcc ttatcttcag atactgtttc agcactaaca ttattagcta tagcaggagc
     4141 aacagacgca gcaatcatgt ccagctcatc aacagcttgg cttgttggag tggagcttac
     4201 ctctgtcaca gtggctgttg ttttagaagt attgtctgag ttatcttcat cgtctaaatc
     4261 atcaaaaact gagcgtttcg gtaatggttg tggtggtgca aacattttac cagcaatcat
     4321 cacggctgca ccaatgacag caactaagcc catagaggca aagacgtcta agcccatact
     4381 tttgccatca gcgtacaata catacataac ttgtaatgtc acaattaagg caataacggt
     4441 tgttttgctt agttcaatat gatcttgcgt ttgtgcaagt gatttagagg gcttggtttc
     4501 cttcattgtt tttatctcaa cagaggttgc taatggcagt gctgaggtag caactggctc
     4561 caaaggtggt tgaactgagt cagatggctc agttgtgttg ataaggttat cttgattttc
     4621 aataatcgga cttaagttaa aaactaaatc atcttctaaa aaatgtgcag ggtctaagat
     4681 cagctcaatc tgttcgggat ggtgcccttg taaactgata gctaagagat gatcaatttt
     4741 agcgagatat tcaccatgcg gatgtaagtg taaatgacgt aatttgacac tttcaggaat
     4801 accgttgtca taacttttaa gttctaaaat aaatcgatgt atttccatcg ataattcggc
     4861 atccaatcca gcaggataaa tcagatcttg ttgaaacatc tgctccaaaa taaaacctag
     4921 cgcatataaa tcaacgctat agtcaatacg ggtaattaag tcagcatggc gttgatgctg
     4981 gtcactcagt aaagcatggc gcattaatgc cgattgagtt gaggcttttt gattaacatg
     5041 aacgggcaaa gggcgagaaa gctttaaagt gttactcacc gcaaacataa aatcaatcag
     5101 acataattta ttaaagttta agtgtaatgg cgtatcaaac gtataaggat gtggtttgta
     5161 agggatacaa atattatcgg ggcgtaattg tgcatgaata acacccgctt ggtgaatttc
     5221 ttttaaagca attaagctat aacgcgcaaa ttgtaaaaac gattgtacat agactaaagg
     5281 atgttttaat tcgtgattat ttgaggcaag tttaggcttt gttcctaacc aatgagccaa
     5341 agaaatgcca gcatctttga tttttatggt ttgataagca tcatctgttt tttcgtcttc
     5401 tttttctttt cgcaaacgag caacatgagg cacacgtttt aacaacatta aaatgttttt
     5461 ttcgcgggca atccaatatt cgttctgagc taagctgtca acgcgtttaa cgtaggtgca
     5521 atagctgtct tcctcgggcg caaggcgttc gaggcgaata aattgtcggt cttgttgatg
     5581 gtcagactcc agacgttcat aacttgtcgc aaataaattg gcaaccgttt tcatgaatgc
     5641 gcaaccttta ggatttggtt gttattattg ttatatgctt tgcagataga cttaaatcgg
     5701 ctcgtcatta tacggtgatt attgcggata cgcaaaacag agttatattt gtgaccgatt
     5761 aaaaaaagca ttctttcaaa caacaataat aacagaactt attaggactt acgcggttat
     5821 cgcttatttg tcgcatttca accgttggta acagttttgt gctcacaaaa tgcacacttg
     5881 aaggtgaagt gcgtaagtac tacttatgta tgacggttct taaatttatc aaaagccgta
     5941 tatagcagaa tggtagtata tctatcgagt gttttacgaa aaa
//
LOCUS       DAOQMB010000108         5958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5780_length_5958_cov_10.565306, whole genome shotgun sequence.
ACCESSION   DAOQMB010000108 DAOQMB010000000
VERSION     DAOQMB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5780_length_5958_cov_10.565306"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /gene="narH"
                     /locus_tag="PLY05_05235"
     CDS             complement(<1..199)
                     /gene="narH"
                     /locus_tag="PLY05_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519317.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNC02742.1"
                     /translation="MKIRAQIGMVLNLDKCIGCHTCSVTCKNVWTSRQGMEYAWFNNV
                     ETKPGIGYPDDWENQERWNGGW"
     gene            complement(199..3948)
                     /locus_tag="PLY05_05240"
     CDS             complement(199..3948)
                     /locus_tag="PLY05_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HNC02743.1"
                     /translation="MSHFVDRLLFFKQQKESFSNGHGVKTEENRQWENAYRNRWSHDK
                     VVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCARGASYSWY
                     LYSANRLKYPMIRGRLVKAWREVRQREKDPVKAWTSLQRDTAVVKDYQAHRGLGGFVR
                     TSWDEVNEIIAAANVYTAKTYGPDRIIGFSPIPAMSMVSYAAGARYLSLIGGTCMSFY
                     DWYCDLPPSSPQTWGEQTDVPESADWYNSAYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYSEVAKLADMWVHGKQGTDAALGMALGHVVLTEHYVKHKTPYFQQYARRF
                     TDMPMLVRLVKKDGLWVAERQLRASDLPNHGGESNNPEWKTVAIDSHSLKVVLPNGSI
                     GSRWGQKGKWNLEEKTLTGEETELLLSLKNHPESGVVSVGFPYFGNIPHEHFVHTSHG
                     DIIPRNVPAITLIDNNGQEMLVATVHDLFLANYGVDQGVGSENVAQNYDDDIPYTPAW
                     QERITGIPRSQVIQLAQEFAETAHLTEGRAMVIVGAGLNHWYHMDMIYRSIINLLVMC
                     GCVGKSGGGWSHYVGQEKLRPQTGWVPLAFATDWVRPPRQMNSTSFFYAHTNQWRYEK
                     LQVQEILSPLANDSEWRNTSLIDCNVRAERMGWLPSAPQLNVNPLTIKALAERAGRNI
                     PDYLTGALKQGQLSMSCEDPDNPVNWPRNMFIWRSNLLGSSGKGHEYFLKHLLGTQNG
                     VLGKDLGEEGGRKPSQVAWHDKAPEGKLDLLVTLDFRMSTTCLYSDIVLPTATWYEKH
                     DLNTSDMHPFIHPLSAAVDPAWESRTDWEIYKGIAKRFSEVCVGHLGVETDVVLNPIQ
                     HDTPLELAQAIDVKEWKKGECEPIAGKTLPSIVTVERDYPNTYARFTSIGPLLEKFGN
                     GGKGIGWDTKKELKLLAELNYTVANGAAKGQPRLDTAIDAAEMILSLAPETNGHVAVK
                     AWAALSEFTGRDHSHLAVPREDDHIRYRDVQAQPRKIISSPTWSGIESEHVSYNAGYT
                     NVHELIPWRTLSGRQQLYQDHPWMRAFGEELCVYKPPVDLKTIKPALDKYSDNRKQVV
                     LNWITPHQKWGIHSTYTDNLIMLTLSRGGPIVWVSEIDAQKANIVDNDWVEVYNVNGA
                     LTARAVVSQRIPEGAIFMYHAQEKLVNIPLSEITQKRGGIHNSVTRTVTKPTHMIGGY
                     AQQAYGFNYYGTVGSNRDEFVILRKMDKVAWGNEGDLASRQQESK"
     gene            complement(3967..5367)
                     /locus_tag="PLY05_05245"
     CDS             complement(3967..5367)
                     /locus_tag="PLY05_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HNC02744.1"
                     /translation="MSSKVLQHWEPENQSFWQQSGHAIANRNLWISIPCLFLAFAVWM
                     VWSMVVVKLPDVGFNYTKNQLFWLTALPALSGATLRIFYSFLVPIFGGRRWTALSTAS
                     LLFPAVGIGYAVQDPNTAYPTMMLLALLCGLGGGNFSSSMSNINFFFPKAEKGYALGM
                     NAGLGNLGVSAMQFIVPIVMTMGIFGALGGEPQAFVKDGVSKDIWLQNAGFVWVPFIA
                     LSAIAAWFGMNDIASAKASFSEQSVIFKRKHNWLMCWLYLGTFGSFIGFSAGFPLLIK
                     GQFPDIDPTKLAFLGPLVGALARSIGGVVADKLGGARVTFWVFLLMVLSVFAVINFMP
                     SKGADGVLVGGNFMGFFVAFLCLFALTGVGNASTFKMIPAIFLTYHERLAQVNKGISR
                     EKVMQDSNKEAAAVLGFTSAIGAYGGFFIPKSYGTSIAMTGSPVAALYTFIVFYAVCL
                     VVTWWFYSRKDAEMPC"
     gene            complement(5380..>5958)
                     /locus_tag="PLY05_05250"
     CDS             complement(5380..>5958)
                     /locus_tag="PLY05_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC02745.1"
                     /translation="FGGYVALALWMVQYYVGEYGFDMKHAALLAACFSLPGGVLRAVG
                     GWLSDKYGAHQVTWWVLWVSWVCLFLLSYPQTDFTIKTVDGTTTFHIGLNVWVFTALM
                     GVVGVAWAFGKASVFKYISDEFPSNIGAVSGVVGLAGGLGGFVLPIMFGALQDITGIR
                     STCFMLLYGVVWVSLIWMYLTEVRRMDLMKVH"
BASE COUNT         1842 a         1460 c         1119 g         1537 t
ORIGIN      
        1 cccagccgcc attccaacgc tcttggtttt cccaatcgtc aggatagcca atacctggtt
       61 tggtttcgac gttgttaaac catgcgtatt ccatcccttg gcgtgatgtc cacacgtttt
      121 tacaagtcac cgagcatgta tggcagccaa tacatttatc gaggtttaac accatgccga
      181 tttgtgcgcg tattttcatt attttgactc ctgctgacga ctggctaaat caccttcatt
      241 accccaagcg actttgtcca ttttgcgtaa aatgacaaat tcatcgcgat tagaacccac
      301 ggtgccgtaa tagttaaaac cataagcttg ttgggcataa ccaccaatca tgtgtgtcgg
      361 tttggtgact gttctggtca ctgagttatg aataccgccc cgtttttggg tgatttcgga
      421 aagtggaatg ttgactagct tttcttgagc gtgatacata aaaatcgcgc cttcaggaat
      481 gcgttgagac accaccgcac gggcagttaa tgcgccattg acgttataaa cttcaaccca
      541 atcgttatcg acgatattag ctttttgtgc atctatttca ctcacccaaa caataggccc
      601 accacgagat aaggtcagca taatcaggtt atcggtatag gtagagtgaa taccccattt
      661 ttgatggggg gtaatccagt ttaaaaccac ttgtttgcga ttatcactgt acttatctaa
      721 cgctggttta atggttttta aatccacagg tggcttataa acacacagtt cttcaccaaa
      781 agcacgcatc caaggatgat cttgatacaa ctgttgacga ccacttaacg tacgccaagg
      841 aatcaactca tggacattgg tataacctgc gttataactg acgtgttctg actcaatgcc
      901 cgaccaagtg ggtgacgaaa taattttgcg cggttgggct tgcacatcac gataacgaat
      961 atggtcatct tcacgcggga cagccaaatg gctatggtct cggcctgtaa actccgacaa
     1021 ggctgcccat gctttaactg ctacatgacc attagtttcg ggggctaaag acaaaatcat
     1081 ttcggcagca tcaatggctg tgtctaaacg cggttggcct ttagctgcac cattggcgac
     1141 ggtgtaatta agctcggcta ataatttaag ctcttttttg gtatcccaac caataccttt
     1201 gccaccatta ccaaattttt ctaacaatgg gccaatggag gtaaaacgtg cataagtgtt
     1261 gggataatca cgctcaacag tgacaatact tggcaaggtt ttacctgcaa taggttcaca
     1321 ttcccctttt ttccattcct tcacatcaat cgcttgcgct aactctaagg gagtatcatg
     1381 ttgaattggg tttagtacca catctgtttc tacgcccaaa tgcccgacac agacttcgga
     1441 gaaacgtttc gcaatgcctt tataaatttc ccaatcagta cgagactccc aagcaggatc
     1501 aacggctgct gacaaaggat gaataaaggg atgcatatca gaggtattta aatcgtgttt
     1561 ttcgtaccaa gttgcggtag gtaatacgat gtccgaatat aagcaagtag tggacattct
     1621 aaaatcgagt gtcaccaata aatccaattt tccttcggga gctttgtcgt gccaagctac
     1681 ttgtgacggt ttacgtccac cttcttcacc taaatcttta cctaaaacgc cattttgtgt
     1741 gcctaataag tgttttaaga aatactcgtg acctttaccc gaagaaccca acaaatttga
     1801 ccgccaaata aacatattac gtggccaatt gacaggatta tcagggtctt cacacgacat
     1861 actgagctgg ccttgtttta atgcgccagt taaataatcg ggaatattgc gtcctgctct
     1921 ttctgctaaa gctttaatgg ttaaaggatt aacattaagt tgtggtgcag aaggcaacca
     1981 tcccatacgc tctgcacgca cgttacaatc aattaaactg gtattacgcc actcactatc
     2041 attagccagt ggcgatagaa tttcttgcac ttgcaatttt tcataacgcc attgattggt
     2101 atgagcataa aagaaggagg tagagttcat ttgacgtggt ggacgcaccc aatccgtcgc
     2161 aaaagctaag ggtacccaac ctgtttgtgg tcgtaatttt tcttgtccca cataatgcga
     2221 ccaaccaccg cccgacttac caacacaacc acacatcacc aatagattga taatactgcg
     2281 ataaatcata tccatgtgat accaatggtt gaggcctgcg ccaacaatca ccattgcacg
     2341 gccttcggta aggtgagcgg tctcggcaaa ctcttgtgct aattgaatga cttgtgagcg
     2401 aggaatcccc gtaatacgct cctgccatgc aggcgtataa ggaatgtcat catcataatt
     2461 ttgtgcaaca ttttcactgc ctaccccttg gtcaacgcca taatttgcca aaaacaaatc
     2521 atgaactgtt gccaccaaca tttcttggcc gttgttgtcg atgagggtaa ttgcaggcac
     2581 attacgtgga ataatatcac catgtgaggt atgaacaaaa tgctcatgag ggatattgcc
     2641 aaaataagga aaaccaacgc tcactacacc actttctggg tgatttttta aactaagcaa
     2701 gagttcagtc tcttcaccag tcaacgtttt ttcttctaaa ttccatttgc ctttttgacc
     2761 ccaacgcgaa ccaattgagc cattaggtaa caccactttt aaactatggc tatcaatcgc
     2821 caccgttttc cattctggat tgttgctctc tccgccatga ttaggtaaat cggacgctct
     2881 taattgacgc tctgccaccc ataagccatc tttttttacc aaacgcacca acattggcat
     2941 atcggtaaaa cgtcgtgcat attgttgaaa atacggtgtt ttgtgtttaa cgtaatgctc
     3001 agttaatacc acatgcccta aggccatgcc caaagcggcg tctgtgcctt gtttgccgtg
     3061 cacccacata tctgctagtt tagcgacttc agaatagtca ggcgttacgg caacggtttt
     3121 agtacctttg taacgaactt cggtaaaaaa atgtgcgtct ggtgtgcgtg tttgtggaac
     3181 gtttgaaccc cacgcaataa tataagccga gttataccaa tcggcagatt ctggaacatc
     3241 ggtttgttcc ccccaagttt gtggacttga tggtggtaaa tcgcaatacc aatcataaaa
     3301 actcatgcaa gtgccgccaa tgagtgacaa ataacgcgcc cctgcggcat aagacaccat
     3361 agacatggct ggaatcggcg aaaagccaat gattcgatca ggcccatagg ttttagcggt
     3421 ataaacattg gcagcagcaa taatttcgtt aacctcatcc catgaagtac gaacaaaacc
     3481 acccaaacca cgatgtgctt gataatcttt aaccactgcc gtatctcgtt gcaaggaagt
     3541 ccatgctttc acagggtctt tttcacgctg gcgcacttcg cgccatgctt tcactaaacg
     3601 accacgaatc atcggatact ttaaacgatt agcactatat aaataccaac tatacgacgc
     3661 accacgtgca caaccacgcg gctcatgatt gggtaaatct gggcgagtac gcggataatc
     3721 ggtttgttgt gtttcccaag tcaccaagcc atttttgaca taaatcttcc aactacacga
     3781 gccagtacaa tttacgccat gtgtagaacg caccaccttg tcgtgtgacc aacgattacg
     3841 gtaggcattt tcccattgac ggttttcttc ggttttaaca ccatgcccat tgctaaatga
     3901 ctctttttgt tgtttaaaaa acaacaatct atcgacaaaa tgactcattc ttgttctcca
     3961 aaaaaattag caaggcattt cggcatcttt gcggctataa aaccaccaag taaccaccaa
     4021 acacactgca taaaacacaa taaaggtata taacgctgcc acgggtgaac cagtcatcgc
     4081 aatggatgtg ccataacttt taggaataaa aaagccacca tacgcaccaa tagcagaggt
     4141 aaaccccaat acagcagcag cctctttatt ggaatcttgc atcacttttt cgcggctaat
     4201 gcctttattg acttgcgcta aacgctcatg gtaggttaag aaaatggcgg ggatcatttt
     4261 aaaggtcgag gcattaccca cacctgttaa ggcaaacaag cataaaaagg ccacaaagaa
     4321 tcccataaag ttaccaccaa ctaacactcc atcagcccct ttgcttggca taaaattaat
     4381 cacagcaaat accgaaagta ccatcaacaa aaacacccaa aaagtaacac gcgccccacc
     4441 gagtttatct gccaccacac caccaataga gcgcgctaat gcacccacta aaggacctaa
     4501 aaaagcaagt ttagttgggt caatatcagg aaactgacct ttaattaaca agggaaagcc
     4561 agcagaaaaa ccaataaacg agccaaaagt ccccaaatac aaccaacaca ttaaccagtt
     4621 atgtttacgt ttaaaaatga ctgattgctc agaaaaagaa gctttggctg aagcaatatc
     4681 attcatgcca aaccaagcag caatggcaga taaagcaata aaaggcaccc acacaaaacc
     4741 tgcattttgc aaccaaatat ctttgctcac cccatctttg acaaaagctt gtggctcgcc
     4801 acctaatgca ccaaaaatcc ccattgtcat cacaattggg acaataaact gcatggcaga
     4861 cacacctaaa ttgcctaaac ctgcattcat accaagggca tatcccttct ctgctttagg
     4921 aaaaaagaag ttgatattcg acattgaaga agaaaaatta ccgccaccta aaccacataa
     4981 taaagctaat aacatcatgg ttggatacgc ggtatttggg tcttgaacag cataaccaat
     5041 cccgactgct ggaaataata acgacgcagt cgaaagagct gtccaacggc ggccgccaaa
     5101 aataggcact aaaaaggaat aaaaaatcct taatgttgcc cctgacaatg ctggcaaggc
     5161 ggttaaccaa aacaattggt ttttagtgta gttaaagcca acatctggca atttaactac
     5221 gaccatactc cacaccatcc acacagcaaa cgctaaaaat aagcaaggaa tagaaatcca
     5281 caaatttcga tttgcaatgg catgacctga ttgttgccaa aaactctgat tctctggctc
     5341 ccaatgttgt aacactttag aagacataaa aattgctcct taatggactt tcattaaatc
     5401 catacgacga acttcggtga gatacatcca aattaaggac acccaaacca cgccatacag
     5461 caacataaaa caagtagaac gaataccagt gatgtcttgt aatgcaccaa acataattgg
     5521 caacacaaaa ccgcccaagc cacctgctaa accaaccacg ccagaaaccg caccaatatt
     5581 gctaggaaat tcatcactga tatatttaaa aacggaggcc ttaccaaacg cccatgccac
     5641 acccacgaca cccatgagcg cggtaaagac ccaaacattt aaaccaatat gaaaagttgt
     5701 tgtgccatca accgttttga tggtgaaatc ggtttgtgga taagacaata aaaacaaaca
     5761 aacccaagat acccacaaca cccaccaagt tacttgatgt gcgccgtatt tgtcagataa
     5821 ccagccgcct acagcacgca atacaccacc tggtaaggaa aaacaggctg ctaacaatgc
     5881 cgcatgtttc atatcaaaac catattcacc cacgtagtac tgcaccatcc ataatgccaa
     5941 agcgacataa ccgccaaa
//
LOCUS       DAOQMB010000109         5955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5787_length_5955_cov_17.048475, whole genome shotgun sequence.
ACCESSION   DAOQMB010000109 DAOQMB010000000
VERSION     DAOQMB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5787_length_5955_cov_17.048475"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..743)
                     /gene="tfpZ"
                     /locus_tag="PLY05_05255"
     CDS             complement(3..743)
                     /gene="tfpZ"
                     /locus_tag="PLY05_05255"
                     /inference="COORDINATES: protein motif:HMM:NF041437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfpX/TfpZ family type IV pilin accessory
                     protein"
                     /protein_id="HNC02746.1"
                     /translation="MTRWRAGFIHLLISLLVVGSVAAYVIYFWYPIPLLPMAKADELL
                     MMVGGIDLIVGPLLTLLVYKQGKKTLKMDLTVIALIQALFLGGGLYTLFASRPVYLVA
                     TEQIFNLVFAADILPADLKKAKNEYQQFGVTQPKLVGAVMPKDAKEVSRIVLSALGGG
                     SDLQHMPQYYVEYSQMKEQILKNALPLINPPKVSSENIAILKKAAISYGYKPEDARFM
                     HVASSRGFAVVLIDAKTAEPIGFVNLDI"
     gene            1013..>1760
                     /locus_tag="PLY05_05260"
     CDS             1013..>1760
                     /locus_tag="PLY05_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC02747.1"
                     /translation="MNQPHYRTCHLCEAVCGVVITTEPSLDGETEVISSIKGDHNDPF
                     SKGHVCAKTVALKSVQEDPDRLRYPLKRTDDGWQEISWQQAFDEVEQGIKRVQSQYGR
                     HAVGFYVGNPTVHNTNALMFVAPLMAALHSKNKFSATSVDQLPHMLAALQMFGHQLLL
                     PIPDIDNTDFMLMLGANPAASNGSLMTAGDVMGRLKKIQARGGRVVVLDPRRTETAQQ
                     VGEHHFIKPASDVYFLAALLNVLFAENLIDE"
     assembly_gap    1761..1860
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1861..>3056
                     /locus_tag="PLY05_05265"
     CDS             <1861..>3056
                     /locus_tag="PLY05_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNC02748.1"
                     /translation="GLSSSSAMGRGSFNRYQSRVRGLPEFNGELPVATLAEEILTEGK
                     GQIKALITHAGNPVLSTPNGTQLEQALDSLDFMVAIDIYLNETTRHAHIILPPTGPLE
                     HGHYDLVFNLLAVRHIAKYSPPLLTPPENTLHDWQILLELTARLSSKTAKDKWLWKGV
                     TAIVERLKPEGILDLLLQIGPYGNLPLGLTPVAERTAKLLVKALSAMPQTQELAYQLQ
                     KILTATPVGGKAQGLSLAKLKDNPHGIDLGALQPSLAQRICTPDHKVILAPHIYLAEL
                     SRARLALNHAKPRQITEFSLIGRRHVRSNNSWLHNSLALVKGKNRCTAMMNSVDAQAL
                     GVQDGDLLTVKSRVGEINIPVETTDDIMQGVLSIPHGFGHHRQGTQLSIAEGKAAGVS
                     MNDITD"
     assembly_gap    3057..3156
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3157..3486
                     /locus_tag="PLY05_05270"
     CDS             <3157..3486
                     /locus_tag="PLY05_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02749.1"
                     /translation="HIRWWNWLGWLFLPALGAFYAWLWQNKRLYVDPMLLLDKLRFGQ
                     VSDIEIAKLAALGNLAFLGCGLLLASLVLLTYAALWTEKQTINILLKPTPETPPTVEE
                     TPSDESK"
     gene            3473..4129
                     /gene="can"
                     /locus_tag="PLY05_05275"
     CDS             3473..4129
                     /gene="can"
                     /locus_tag="PLY05_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNC02750.1"
                     /translation="MSQNSLEHLLAKNKQWAQRIKDDDPSFFQQLSSQQKPRYLWIGC
                     ADSRVPCTQLVDLLPGDMFVHRNVANLVIHTDFNCLSVMQYAIDVLQVEHIIVCGHYR
                     CGGIQAALQNQQFGLIDNWLRHVHDIITKYHTYLDKFATEQERLDQLCELNVQEQVFN
                     VCENTLVRHAWQRGQKLTVHGWIYGVEDGLLRDLAVSVSSTEQMKSSIHHLFTKTGLI
                     "
     gene            4299..5492
                     /locus_tag="PLY05_05280"
     CDS             4299..5492
                     /locus_tag="PLY05_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HNC02751.1"
                     /translation="MRLADYCSAEEWKRIKEFADTKETPFLVVDLEIIKQKYEELTTC
                     FPMAKVHYALKANPGKPVIELLRDLGSNFDIASIYELDKVLATGVDADRISYGNTIKK
                     AAHIKYAFDKGVRLYATDSKADLQSIAENAPGSKIFVRILVEGGETAEWPLSRKFGCH
                     PDMAIDLLVQAKQLGLIPYGISFHVGSQQKDIAVWDAALSKVKYMFDWMRLEEGVMLK
                     MINMGGGFPANYISEVNPIQVYAEEILRYLHDDHGDELPELILEPGRSLVGESGVLVS
                     EVVLISRKSRTDLKRWIYTDVGLFQGLIETMGEAIKYPIYTEKMGADVGEGSVVLAGP
                     TCDSTDIMYENANYHLPHNLAVGDRLYWMTTGAYTTSYSSVEFNGFPPLKTYFIGCGK
                     EEEKN"
     gene            complement(5545..>5955)
                     /locus_tag="PLY05_05285"
     CDS             complement(5545..>5955)
                     /locus_tag="PLY05_05285"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC02752.1"
                     /translation="AAKLDKAKVFILAIDDVSDSIRTAMQVRHYFPHLTILARARDRR
                     HAYQLMDIGVHVVTRELFASSLDVAQKALIEAGLSPERAAQGIQKFREYDEQLLIRQQ
                     AFYQDEESLIASNKQAMRDLEELFESDERAAENS"
BASE COUNT         1705 a         1041 c         1232 g         1777 t
ORIGIN      
        1 tatcaaatat ctaaattaac aaaaccaata ggctctgctg ttttagcatc tattaaaaca
       61 acggcaaaac ctctactact tgctacatgc ataaagcgcg catcttcggg tttgtagcca
      121 taactgatag cggctttttt caagattgca atattctcgc tcgatacttt aggtggattt
      181 ataagcggta aagcgttttt tagaatttgt tctttcatct gactatattc aacataatat
      241 tgaggcatat gttgtaaatc gctgccacca cccaatgcag atagcacaat acggctgact
      301 tcttttgcat ctttaggcat cactgcgcca actaatttag gttgagttac tccaaattgt
      361 tgatattcat ttttagcttt ttttaggtcc gcaggaagaa tatcagccgc aaaaactaaa
      421 ttaaatattt gctctgtagc cactaaatag actgggcgag aggcaaagag ggtatataaa
      481 ccaccaccta aaaacagggc ttgaattaat gcaataaccg ttaaatccat ttttaaggtc
      541 ttttttcctt gtttgtatac aagtaaagtg agtaatgggc caacaattaa atcaattcct
      601 cctaccatca tcagtaattc atcagctttt gccatgggta ataaaggaat tgggtaccaa
      661 aaataaatga cgtaagccgc aactgaacca acaactaata agctaattaa taaatggata
      721 aaaccagcgc gccaacgagt catatcttgc actcctctta ttttgatttt tgaaaggcgg
      781 ctaagtgtaa ctgacatgtt ttataacgca aagtgtttta cattagagag gtaatagggt
      841 actttcgtgc cattagatca atcaacaata agaactgaac ttaggcattg caccgcaatt
      901 agcgcatttt gtgaatataa agccgttaaa agcggctgtc atgtgagtaa aaaagtgata
      961 gctgcctaaa ttctaaagaa gttttaagcc aaattgccaa gaggattgtc ttatgaatca
     1021 gccccattat cgtacctgcc atttatgtga agctgtgtgt ggtgtcgtta ttaccacaga
     1081 gccaagttta gatggcgaaa cagaagtgat tagtagtatt aaaggtgatc ataatgaccc
     1141 gtttagcaaa ggacatgttt gtgccaaaac agtggcatta aaaagcgtgc aagaagaccc
     1201 agaccgttta cgttatccct taaaacgcac ggatgatggt tggcaagaaa ttagttggca
     1261 acaagctttt gatgaagtcg aacaaggtat taaacgtgtg caaagccaat atggtcgtca
     1321 tgccgttggc ttttatgtag gaaatccaac ggttcataat acgaatgcac tgatgtttgt
     1381 tgcaccatta atggctgcct tacatagtaa aaataaattt agtgcgacta gtgttgacca
     1441 attgccacat atgttggcag cattacaaat gtttggccat caattattat tacccattcc
     1501 agatattgat aatactgatt ttatgctcat gcttggggca aatcctgcgg catctaatgg
     1561 aagtttaatg actgctggcg atgttatggg acgattaaag aagattcaag cacgcggtgg
     1621 tcgagtagtg gtgttagacc cacgacgtac cgaaactgca cagcaggtag gtgagcatca
     1681 ttttattaaa cctgccagcg atgtttactt tttagcggct ttgttaaatg tcttatttgc
     1741 cgaaaacttg attgatgagt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 ggtttaagtt cttctagtgc aatggggcgt ggcagtttta atcgttatca atcgcgtgtg
     1921 cgtggtttac ctgagtttaa tggcgaatta cctgtagcaa ctttagctga agaaatttta
     1981 accgaaggta aaggccaaat taaggcgtta attacccatg caggtaaccc cgtcttatca
     2041 acgcccaatg gcacacagct tgagcaagca ttagacagct tagactttat ggtggcaatc
     2101 gatatttatc tgaatgaaac cacccgtcat gcacatatta ttttgccgcc aacaggtccg
     2161 ctagagcatg gccattatga tttagtattt aatttattgg cagtacgtca tattgccaaa
     2221 tattcacccc ctttattaac gcctcccgaa aataccctac atgattggca aattttatta
     2281 gaattaaccg cgcgtttaag tagcaaaaca gctaaagata aatggttatg gaaaggtgtt
     2341 accgcgattg ttgagcgtct aaaaccagaa ggaattttag atttattatt acaaattggg
     2401 ccttatggta atttaccctt aggtttaacg cctgtagccg agcgtacggc taaattatta
     2461 gttaaagctt tatcagcgat gcctcaaacc caagaattag cgtatcaatt acaaaaaatc
     2521 ttaacggcaa cgcctgttgg tggcaaagca caaggtttga gtttggcgaa attaaaagat
     2581 aatccacatg gcatagattt aggtgcatta caaccttctt tagcacaacg tatttgtaca
     2641 cctgaccata aagtcatttt agctccgcat atctatttgg ctgagttaag ccgcgctcgt
     2701 ttggcgttaa atcatgcaaa gcccagacaa attaccgagt ttagcttgat tggtcgtcgc
     2761 catgtgcgta gcaataattc atggttacac aatagtttag ctttggtgaa gggtaaaaat
     2821 cggtgtacag ccatgatgaa tagcgttgat gctcaagctt taggtgtgca agatggtgac
     2881 ttgttaactg ttaaatcaag agtgggagaa attaatatcc ctgtagaaac gaccgatgat
     2941 attatgcagg gcgtattaag tattccgcat ggttttgggc atcatcgtca agggactcaa
     3001 ttgagtattg ccgaaggcaa agcagcagga gtgagtatga atgatattac cgatgannnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncata ttcgctggtg gaattggttg
     3181 ggttggttat ttttacctgc cttgggcgcg ttttatgcgt ggttatggca aaataagcga
     3241 ctgtatgttg accctatgtt gttattagat aaattacgct ttggtcaagt gagtgatata
     3301 gaaattgcca aattagcggc tttgggcaat ttagcatttt taggttgtgg tttattatta
     3361 gccagtttag tgttattaac ctatgccgcg ttatggacag aaaaacaaac gattaatatc
     3421 ttactcaaac caacacctga gactccaccg actgtagagg aaacccctag tgatgagtca
     3481 aaatagcctt gaacatttat tggccaaaaa taaacaatgg gcgcaacgta ttaaggatga
     3541 tgaccctagt ttttttcaac aattaagctc tcagcaaaaa cctcgttatt tgtggattgg
     3601 ctgtgctgat agccgtgtac cgtgtactca attagtcgat ttattacccg gtgatatgtt
     3661 tgtccatcgt aatgtcgcta atttagtgat acatacggac tttaattgtt tatcagtcat
     3721 gcaatatgca attgatgtat tacaggtcga gcatattatt gtttgtggtc attatcgttg
     3781 tggtggaata caagcggcat tacaaaatca acagtttggt ttaattgata actggttgcg
     3841 tcatgtacac gatattatta ccaaatatca tacctattta gataagtttg cgacagagca
     3901 agagcgttta gaccaattat gtgagttaaa tgtccaagaa caagtgttta atgtttgtga
     3961 aaatacccta gtgcgtcatg cgtggcaacg tggtcaaaaa ctgacggtac atggctggat
     4021 ttatggtgtt gaagatggcc tattacgcga tttagcggtg agtgttagct ccactgagca
     4081 aatgaaaagc tccattcatc atttgttcac aaaaacagga ttaatttagc caacataaca
     4141 gcgattgtct cttgattatc gggggctttg gccttaatct tcgcaccttt acggagacaa
     4201 ccgtgtttca ccgcttttga gtattcataa gcattgtttg gggagtgtgt gacaacatac
     4261 aatcccagtg ttgttgcttg ccttgacggg tatatcttat gcgcctagct gattattgtt
     4321 ctgccgaaga atggaaacgt attaaagagt ttgctgatac taaagaaaca ccatttttgg
     4381 tggttgattt agaaatcatc aaacaaaaat acgaagaact cactacttgt tttccaatgg
     4441 ctaaggtgca ttacgcgctc aaagctaacc ctggtaaacc agtcattgaa ttattgcgtg
     4501 atttagggtc taactttgat attgcctcta tttatgagtt agataaagta ttggcaacgg
     4561 gcgttgatgc tgaccgtatt tcttatggta ataccattaa aaaagcagca catattaagt
     4621 acgcttttga taaaggtgta cgtttatatg caaccgactc aaaagcagat ttgcagtcaa
     4681 ttgccgaaaa tgcaccgggt tctaaaattt ttgtgcgtat tttggtagaa ggtggcgaaa
     4741 cagccgaatg gcctttgtcg cgtaaatttg gttgccatcc cgatatggca attgatttat
     4801 tggtacaagc caagcaatta ggtttaattc cttacggtat ttctttccac gttggctcac
     4861 agcaaaaaga cattgcggtg tgggatgcag ccttatctaa agtcaaatat atgtttgact
     4921 ggatgcgtct tgaagaaggc gtgatgttaa aaatgattaa catgggcggt ggttttcctg
     4981 ccaactatat ttctgaagtt aacccgattc aggtctatgc tgaagaaatc ttacgttatt
     5041 tacatgatga ccacggtgat gaattgccag agttgatttt agagcctggt cgttctttgg
     5101 tgggtgaatc tggtgtatta gtttcagaag tcgtattgat ttcgcgtaag tctcgtaccg
     5161 atttaaaacg ttggatttat actgatgttg gtttgttcca aggcttaatc gaaactatgg
     5221 gtgaagccat caagtatcct atttataccg aaaaaatggg tgcagatgtg ggtgagggta
     5281 gcgtggtttt agcaggccca acgtgtgact ccaccgatat tatgtatgaa aatgccaatt
     5341 atcatttgcc acacaattta gcagtgggtg atcgtttgta ttggatgact actggtgctt
     5401 atacaacctc ttatagttct gtagagttta atggtttccc accattaaaa acctacttta
     5461 ttggttgtgg taaagaagaa gagaaaaact aagattaatt ggtttttaga taatagaaaa
     5521 atcccccatt tttgggggat ttttttaaga gttttcagct gctctttcgt cactttcaaa
     5581 caactcttct aaatcacgca tggcttgttt attactggcg ataagtgatt cttcatcctg
     5641 ataaaaagcc tgttggcgaa tcaatagttg ttcatcatat tctctaaact tttgtatgcc
     5701 ttgtgcggca cgttcaggac ttaaaccagc ctcaattaag gctttttgag caacatctaa
     5761 cgaagaagca aacaattcac gggtgacaac atgcacgcca atatccatta gttgataagc
     5821 atgacgacga tctcgcgcac gtgccaaaat ggttaaatgc ggaaagtagt gccgaacttg
     5881 catcgcggtg cgtatggagt cactgacatc atcaatcgct aaaataaaca ctttggcttt
     5941 gtcgagtttg gcggc
//
LOCUS       DAOQMB010000110         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5806_length_5945_cov_16.378098, whole genome shotgun sequence.
ACCESSION   DAOQMB010000110 DAOQMB010000000
VERSION     DAOQMB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5806_length_5945_cov_16.378098"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PLY05_05290"
     CDS             complement(<1..284)
                     /locus_tag="PLY05_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02753.1"
                     /translation="MSDSEMMSIKDTSKNNNYSNTIQQASSIIHVAMRSAQELTHLVA
                     EVHSTINALPSPFNKHHQPSAKYAPFPYKIVSTAFSLLAKLSHILAPQST"
     gene            474..1268
                     /locus_tag="PLY05_05295"
     CDS             474..1268
                     /locus_tag="PLY05_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNC02754.1"
                     /translation="MSFNLRFSPVLLILMTSSAYAAPLQICVDKIPNPPFAYSEKIQG
                     AGKLKGYSLDIIQKALKTMAIPYAVKLVSSAEIAKKMQDANPNAGCDIALDVQKGSSL
                     ESGVLLTHPIYRLHFDLVYNWETYMSGLGIKNLADMNKFNVCGVKNVDYGALNKQLKI
                     QEHNTIKDVMFNIKAKECDVFVAEGVLMRYGQRANQYQVPPVGCIRLSGTEKSYHIAV
                     SKHVPNASNFVGQIQQAIGTLSKDIAVLAEEYDVNPIKCQQTLNIN"
     gene            1222..2064
                     /locus_tag="PLY05_05300"
     CDS             1222..2064
                     /locus_tag="PLY05_05300"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNC02755.1"
                     /translation="MMLTLSSVSKHLTLISRSFLWLAGVGICLVASQVQAAKWQVCLD
                     ENPWPPYSYPISEQDNQLTGYTVDIATQVLSYLRLDYEFIRLPWAEVHQRAKNYQKNA
                     QCDMVLDVSLTPERQSYLYFSQPLYQLKYSLVYDNGRVDVANLRQNIKTRQFCGVQGY
                     NYGVLADFLAIRRLDSIQNVLDALNNKQCDFFIIEAAVLQNAIKKQLYQFSNMGCINL
                     EGATKTYRLGIAKKTPQSVAYSAAIDKHINLLRRQGVLSQLTDHYSLEAKSCQGSLSL
                     NSLR"
     assembly_gap    2135..2234
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2506..3537
                     /gene="argC"
                     /locus_tag="PLY05_05305"
     CDS             2506..3537
                     /gene="argC"
                     /locus_tag="PLY05_05305"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNC02756.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAQHPQVELKAITSRSEAGMPVSQ
                     MFPNLRGRVDLPFTEPTIENLGDCDVVFFATPNGVAMKQVPELLDKGIKVIDLAADYR
                     LKNPAEWQKWYGMAHASENLLAEAVYGLPEVNREAIKSARLVANPGCYPTAVQLGFLP
                     LLKAGLIDTQSLVADAKSGVSGAGRKAEVHTLLCEAGDSFKAYGVAGHRHLPEIKQGL
                     AYISGQNVGLTFVPHLLPMIRGIHATLYARLTGDIADLQALYEQTYANEAFVDVLPAG
                     SHPETRTVRGANMCRMAVHRPQGGDTVVILSVIDNLVKGAAGQAIQNMNIMCGLPETT
                     GLNHIALLP"
     gene            3626..4315
                     /locus_tag="PLY05_05310"
     CDS             3626..4315
                     /locus_tag="PLY05_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02757.1"
                     /translation="MWQSGRRQRLKSYLNSPITQHLLAWSLVAILGLVVNAIGFFYWG
                     YDYGLRSIGASANEVQQLRQQVKQQNQQIPLLQQQLAIAQQNLDIAQKTSQQLQQDNK
                     SQLASVADMQEQIMVYQRLLGTKGAANALNIDSFNVQRLTNGSYQYRVLLTQPQNNKQ
                     NVRVIVKIIPNNKQAPVILQSEEQSFQFFKSITGEFVLPSGFVVDSVEVTIQGVGKKA
                     IKLQKRFKWDL"
     gene            4312..>5945
                     /gene="recD"
                     /locus_tag="PLY05_05315"
     CDS             4312..>5945
                     /gene="recD"
                     /locus_tag="PLY05_05315"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNC02758.1"
                     /translation="MSFLHLPIQRSEFGSAWADSLATLLVQLHEGQGGNRQHSIRLAG
                     YVRDLCAALDHGHSCLYSEFFDRLADYQSPIIVPVEQALQHIAPLVLEDKRLYLYRYW
                     WDEYRLAQAIKQLNRHIPSTLADADLQQLSVGTHPQQAQAIKVALQSGFTLITGGPGT
                     GKTFTLVRILIALLQQNSQLTVALAAPTGKAAARMQEALRQSLAQLPVAQQLLKALPQ
                     TAFTLHRLLGMGHGTQPKFNAQQPLPYDLIIVDEASMIDLRMASQLLTAISPNARLIL
                     LGDANQLAAVEAGAVLAELNKAQTLSQSRVHLTQSQRFGGQIGQLAEAVCRGDTLTVT
                     QLIEQSSDELNYAPLEQVESLAKKLFIGYQAFAQALKSKTDIVDLLKLFDDFRVLCAV
                     REGSYGVNALNQRLSLLLQKELVQNEDPLAVWYHGRPVMVTQNDYSLDVFNGDIGITL
                     EEEDGFYVYFPARDTPTKRVSAARLAHSETALALTIHKSQGSEFKQVAVVLPKEESPI
                     LTRQLLYTGITRAKKQIQLWALKPILLKTIEQETKRA"
BASE COUNT         1793 a          999 c         1262 g         1791 t
ORIGIN      
        1 tctgtggatt gaggagctaa aatatggctt aacttggcta ataaagaaaa agcagtggac
       61 acaattttat aagggaatgg tgcgtatttg gcactgggtt gatgatgttt gttaaaaggt
      121 gagggtaagg cattaatggt gctatgtact tctgcaacca aatgagttaa ctcttgtgca
      181 ctacgcatgg caacatgaat aatagagctt gcctgttgaa tagtattaga gtagttgttg
      241 tttttagatg tgtctttaat actcatcatt tcgctgtcag acatgataaa cctctaaatt
      301 ggtgtacata tgttcactaa tttagagggc ttttgtgttt ttgtcaagtc gtgttggatt
      361 gtttattaag taatcattct cgacactgat tgtcaaacac gctagactga atagagcaga
      421 tagtttcgca aggatgccct caatccattc attataaaaa tcggagggtt tatatgtctt
      481 ttaatttacg ttttagtcct gttttgctga tattgatgac gtcttcggca tacgccgcac
      541 cattacaaat ttgtgttgat aaaataccta atccaccatt tgcctattca gaaaaaatac
      601 aaggcgcagg taagttaaaa ggttatagtt tagacattat tcaaaaagca cttaaaacga
      661 tggcgatacc ttatgcggtg aaattagtct ctagtgccga gattgctaaa aaaatgcaag
      721 atgccaaccc aaatgcaggt tgtgatattg ctttagatgt acaaaaaggc agtagtttag
      781 aatcaggtgt tctgttaacc catccaattt atcgcttaca ttttgatttg gtttataact
      841 gggaaaccta tatgtcaggt ttgggtataa aaaaccttgc tgatatgaat aaatttaatg
      901 tttgtggtgt taaaaatgta gattatggcg cattaaataa acaactaaaa attcaagaac
      961 ataatacaat taaagatgtg atgtttaata ttaaagccaa agagtgtgat gtgtttgtgg
     1021 ccgaaggagt attgatgcgt tatggccaac gtgccaacca ataccaagtg ccacctgttg
     1081 gttgtattcg cttatcaggc accgaaaaaa gttatcatat tgcggtatct aagcatgtgc
     1141 ctaatgccag taattttgtc gggcaaatac agcaagcaat cggcacatta agcaaagata
     1201 ttgctgtatt ggcagaggaa tatgatgtta accctatcaa gtgtcagcaa acacttaaca
     1261 ttaattagcc gcagtttttt gtggttagct ggtgttggta tttgtttggt agcttcgcaa
     1321 gtacaagcgg ctaagtggca agtttgttta gatgaaaacc cttggccgcc atatagttat
     1381 cccatttctg aacaagataa tcagttaaca ggttatacag tcgatattgc aacacaggta
     1441 ttatcttatt tgcgtttgga ctatgagttt atcagattgc cgtgggcaga agtgcatcaa
     1501 cgcgccaaaa attatcaaaa aaatgcacag tgtgatatgg ttttagatgt tagtttgact
     1561 cccgaaagac aaagctattt gtactttagc cagcctttgt atcaattaaa atatagctta
     1621 gtttatgata atggccgtgt ggacgtagca aatttaagac aaaatattaa gactcggcaa
     1681 ttttgtggtg tacaaggtta taactatggt gtgctggcag actttttagc gattcgtcgt
     1741 ttagattcaa tacaaaatgt tttggatgct ttaaataaca aacaatgtga tttttttatt
     1801 attgaagcgg cagtattgca aaatgccatc aaaaaacagc tttatcaatt tagtaatatg
     1861 ggatgtatta atttagaagg ggcaacaaaa acttatcgct taggcattgc taaaaaaaca
     1921 ccacagtccg ttgcctatag tgcagctatt gataaacaca tcaatttatt aagacggcaa
     1981 ggcgttttaa gccaattaac agatcactat agtttagaag ccaaaagttg tcagggcagt
     2041 cttagtttaa atagcttgcg ctaacgtagg ctagtcattg tcgaaggttg taagcaacct
     2101 tgccataatt gataaatcgc ttgttctaac tcatnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnaataaa atcagcacga cgtaataatt tgtcagcatc agtcattgca
     2281 ttacgcgcca taaatatctc ctagtttttg gctaattatt cttttttttg atgataagta
     2341 ttgattttgt aggccaattt aacagaattt tttgttaaaa gatgacttga gaataacaag
     2401 attttaaaga tagacaaatc attttttaac aaaagctttg ctgttagatg ttttaatagc
     2461 tcgttataat acagcagccc ctattaagtc ttttggagat agagagtgat taaagttggt
     2521 attgttggcg gtacaggtta taccggtgtt gaattattgc gtttattagc acaacaccca
     2581 caagttgagt taaaagccat tacctcgcgt agcgaagcag gtatgcctgt atcgcaaatg
     2641 tttcccaact tacgtggtcg agtagattta ccatttaccg aaccgactat cgaaaattta
     2701 ggcgactgtg atgtggtatt ttttgctacg cctaatgggg ttgccatgaa gcaagtccca
     2761 gaattattag acaagggcat taaagttatt gatttagccg ccgattatcg cttaaaaaat
     2821 cctgctgaat ggcaaaaatg gtatggtatg gcgcatgcca gcgagaattt attagcagaa
     2881 gcggtgtatg gactgccaga agtcaatcgt gaagccatta aaagtgcgcg tttagtggct
     2941 aatccaggtt gttatccgac agcagtgcaa ttaggctttt tgcctttatt aaaagcaggg
     3001 ctgattgaca cacaaagttt agtggctgat gctaaatcgg gtgtgtcggg ggcaggtcgt
     3061 aaagctgaag tgcatacctt attgtgtgaa gcaggcgata gttttaaagc ttatggtgtt
     3121 gctggccatc gtcatcttcc cgaaattaaa caaggtttag cgtatattag tggccaaaac
     3181 gtgggtttaa cctttgtacc acatttattg ccaatgattc gtggtattca tgcaaccttg
     3241 tatgcgcgtt taacaggtga cattgcagat ttacaagcac tttatgagca aacttatgca
     3301 aatgaagcgt ttgtggatgt attacctgca ggcagccatc cagaaacacg cacggtgcgc
     3361 ggtgcaaata tgtgtcgtat ggcggtgcat cgtccacaag gtggcgatac cgttgtcatt
     3421 ctttcggtga ttgataactt agtaaaaggt gcagcaggtc aggctataca aaacatgaat
     3481 attatgtgtg gtttacccga aacaacgggc ttaaatcata ttgccttgtt accctaaagt
     3541 gttttttctc cctcgcccct ttagggagat gattggggtg aggggataac taaaatatta
     3601 ctattatgat tgtaggataa aaattgtgtg gcaaagtgga cgcagacaac gactaaaaag
     3661 ttacctaaat agtccaatta ctcagcattt attagcatgg agtttagttg ccatattagg
     3721 tttagtagtc aatgcgattg gcttttttta ctggggttat gattacggtt tgcgtagcat
     3781 tggtgcgagt gccaatgaag tccaacaatt gcggcaacaa gttaagcagc aaaatcaaca
     3841 aattccgctt ttgcaacagc aattagcaat tgcccagcaa aatcttgata ttgcccaaaa
     3901 aacctcgcaa caacttcagc aagacaacaa aagtcaatta gccagcgttg ccgatatgca
     3961 agagcaaatc atggtatatc aacgtttatt aggcactaag ggagcagcta acgccttaaa
     4021 tattgatagt tttaatgttc agcgtctcac taatggtagt tatcaatatc gagtattact
     4081 cacacaaccg caaaacaata aacaaaatgt aagagtgatt gtaaaaatta tacccaataa
     4141 caaacaggcg ccagttatct tgcaaagtga ggaacaaagt tttcaatttt ttaaaagtat
     4201 tacgggtgaa tttgttttgc cgtcaggttt tgtcgttgat agtgttgaag tcactataca
     4261 aggtgtaggt aaaaaagcta ttaaactaca aaaacgattt aagtgggatt tatgagcttt
     4321 ttacatcttc ctatacaacg tagtgagttt ggttcggcat gggcagatag tttagccacc
     4381 ttgttagtac aattacatga agggcaaggt ggtaatcgtc agcacagcat aagattggct
     4441 ggttatgtgc gtgatttgtg tgccgcttta gatcacggtc atagttgttt atatagtgag
     4501 ttttttgatc gtttggcaga ttatcaatcg cctattattg tgcctgttga acaagcttta
     4561 cagcatatag ctcccttagt tttagaagat aaacgcttat atttatatcg ttattggtgg
     4621 gatgaatatc gtttagcaca agccattaag caactcaatc gacatattcc aagcacatta
     4681 gccgatgcag atttgcaaca actttccgta ggcactcatc cacaacaagc gcaagcgatt
     4741 aaagtcgcgt tacaaagtgg ttttacactc attacgggtg ggccgggtac aggtaaaacc
     4801 tttaccttag tacgaatttt aatagcatta ctacagcaaa attcacagtt aaccgttgct
     4861 ttggctgccc caacaggtaa agctgctgca cgaatgcaag aagctttacg tcagagtttg
     4921 gcgcaattac ctgtagcgca gcagctacta aaagctttac cacaaacagc ttttacattg
     4981 catcgtttgt taggaatggg gcatggcacg cagcctaagt ttaatgcaca gcaaccgtta
     5041 ccttatgatt tgattattgt tgacgaagca tccatgattg atttgcgtat ggcaagtcaa
     5101 ctgctcaccg cgattagccc aaatgctaga ttgattttgt taggtgatgc caatcagttg
     5161 gcagcagttg aagcaggagc tgtattggca gaactcaata aagcccaaac actcagccaa
     5221 agtcgcgtac atttaacgca aagtcaacgt tttggtggac aaattggtca attagccgaa
     5281 gcggtatgcc gtggtgatac gctaacggtg actcaattaa ttgaacaaag tagtgatgag
     5341 ctaaattatg cacctttaga gcaagttgaa tcattagcta aaaagctctt tattggttat
     5401 caagcctttg cacaggcttt gaaaagtaaa actgatattg ttgatttatt aaaactcttt
     5461 gatgacttta gggtgttatg tgcagtacgc gagggaagtt atggggtcaa cgcactaaat
     5521 cagcgtctta gtttattgtt gcaaaaggaa ttggttcaaa atgaagaccc tttagctgtt
     5581 tggtatcatg gtcgtcctgt gatggtaacc caaaatgatt atagtcttga tgtttttaat
     5641 ggtgatattg gcattacgct tgaggaagaa gatggatttt atgtctattt tccagcacga
     5701 gacactccaa ctaagcgtgt gtcggcggca cgtttggcgc acagtgagac ggcattagca
     5761 ttaacgattc ataaatctca aggctcagag tttaaacagg ttgcagtggt tttgcctaaa
     5821 gaggagtcgc caattttaac gcgtcagtta ttatatacag gcattactcg agccaaaaag
     5881 caaattcaat tatgggcatt aaagccaatt ttgctaaaaa ccatagaaca agaaaccaaa
     5941 agagc
//
LOCUS       DAOQMB010000111         5945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5807_length_5945_cov_15.394058, whole genome shotgun sequence.
ACCESSION   DAOQMB010000111 DAOQMB010000000
VERSION     DAOQMB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_5945_cov_15.394058"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="PLY05_05320"
     CDS             <1..219
                     /locus_tag="PLY05_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02759.1"
                     /translation="RLGIASGAVPEKGKYIVILMDQLDSGIGFSIAYWWYLSLSIQQT
                     LLCALTFPITALIVKRLLFMARLKKSAT"
     gene            complement(272..1489)
                     /locus_tag="PLY05_05325"
     CDS             complement(272..1489)
                     /locus_tag="PLY05_05325"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNC02760.1"
                     /translation="MSCPSHGLMSVDEVLQRLQQAASQKTPVIEQTLLSDALGRVLAE
                     DVVATIDVPPQANSAMDGYAVYTDDLQHTTILAISQRIAAGSAPQPLQRGTAARIFTG
                     AVIPDGANAVVMQENTQLTADGLVEVLQRPDAQENIRPQGQDIACGVTILRQGEKLTP
                     AAIGLLATIGKAKVKVYQRLKVVYFCTGDELAQAEQTLKVGQIYNSNSPLLGALLTEQ
                     GYQAIDGGVIADNLPDTINALANAAQQADVVMTTGGVSVGEEDHVKAALQQIGQLDLW
                     KVALKPGKPLAFGSIGTTPFIGLPGNPQSVFVTFKVMAQTFLAYYQGQRHKVKPSVVA
                     MPIDFSIKKAQGRQEYLRVRLVQNEQGEWVLSKYHNQSSGVLSSAVWADGFAIIEIGK
                     TYERGDSVAFWRF"
     gene            complement(1489..2031)
                     /gene="moaB"
                     /locus_tag="PLY05_05330"
     CDS             complement(1489..2031)
                     /gene="moaB"
                     /locus_tag="PLY05_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HNC02761.1"
                     /translation="MSHSAINKPFVPLQIAVLTVSDTRNLETDTSGQYLIDALTGAGH
                     LLADRQIVKDDIYQIRAIISAWIASPNVQAILVTGGTGFSGRDSTPEAVNVLFDKHIE
                     GFGEIFRHYSMIEIGTSTLQSRALAGMANNTVIFCMPGSTNACKTAWINILSQQLDNQ
                     HRPCNFVPNLKKAELCGSRG"
     gene            complement(2149..3144)
                     /gene="moaA"
                     /locus_tag="PLY05_05335"
     CDS             complement(2149..3144)
                     /gene="moaA"
                     /locus_tag="PLY05_05335"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNC02762.1"
                     /translation="MSNPALIDGFGRQITYVRLSVTDRCDFRCVYCMGEDMVFLPRQQ
                     ILTLEELYRVGRVLVSLGVSKIRLTGGEPLVRQNIVWLVEKLASLQGLKELTLTTNGS
                     QLTRLAQPLKEAGLSRINISLDSLQEQRFSQITRTGKLSEVLAGIEAAQQAGFQHIKL
                     NSVILKGKNDDEILDLVNFIRQNQLDIAFIEEMPLGVITEHQRAETYISNDQVRHSID
                     QFYPLTAVSESTGGPARYYHMSDSQSRIGFISPHSHNFCGSCNRVRVTAEGRLLLCLG
                     NEHSADLKAPLRDFPHDDQPLIDTLFNALPLKPEKHHFDLNDEPQIVRFMNMTGG"
     gene            complement(3283..5925)
                     /gene="pepN"
                     /locus_tag="PLY05_05340"
     CDS             complement(3283..5925)
                     /gene="pepN"
                     /locus_tag="PLY05_05340"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HNC02763.1"
                     /translation="MTNPKTVYLKDYQAPLFSIEQVNLQVDIFNDYTLVTSTLNLTPQ
                     QKNQALVLHGVDLELQNLHLDNQLLSIDDYQLQGEELVIHNVPNQPFSLQSVVKIYPA
                     KNLALEGLYLSKGMFCTQCEPEGFRKISYYLDRPDVLSSFTTTITADKNQYPTLLANG
                     NLIAQGDAGEGRHFATWQDPFKKPSYLFAMVAGDLACLEDSFTTMTGREVALKIYVEP
                     HDLRQCHHAMESLKASMRWDEQVYGREYDLDIFMIVAVSHFNMGAMENKGLNIFNTSC
                     VLAHPETTTDLGFQRVESVVAHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDQQFSAD
                     QLSASVQRIEDVAVLRNVQFAEDASPLAHPVRPESFIEINNFYTATVYEKGAEIVRML
                     HTVLGADAFRKGTDLYFSRHDGQAVTVEDFVNALAQANNTDLSAFMQWYKQPCTPVLT
                     VNGQYDAKSKTYTLNVSQKTPHKQGFDEPQLLPIPVKLALFDAQTGRAIALKLAHETQ
                     AMGKERVLIADAAEQQWVFTGIERGVVPSLLRDFSAPVILDYQTTDQELLFLMQYDDN
                     GFNRWLSAQSLFERMLLTMIANINAQKDAFDNTNIDNVLTALAELLPELAEQDPALAA
                     KLLSLPIENYLAEKTAVIYPARIHQARNMLQAEIANALECFFSEQYAKNANDSDYIYN
                     AQAIAIRAWRDVCLRYLLATVDNKIPQQLAVQQYQQAQNMTERMCALRGLVHSQAPQT
                     AECLQDFYWRFEQEALVIDSWFAVQATNPQASLSDIKALTEHQAFSLTNPNRLRSVLA
                     QFANSNPVLFHQQNGDGYRFVTQKIAELDKLNPQIASRLLGAFSRWQRLRPTLKQQAQ
                     AALQKLREQSLSNDTFETLNRLLA"
BASE COUNT         1773 a         1243 c         1161 g         1768 t
ORIGIN      
        1 cgtttgggta ttgcttcggg tgctgttccc gaaaaaggta aatatattgt gattctcatg
       61 gatcaattag attcaggtat tggttttagt attgcttatt ggtggtattt atccttatcc
      121 atacaacaaa cattgttatg tgctttaact tttcctatta ctgctttgat tgtaaaaaga
      181 ttattgttta tggcgagatt aaaaaagagt gcaacttaga ccaagactta cgcagttatc
      241 acttatttgc tgaaatgcgt aagtcctcag tttaaaatcg ccaaaaagcc acgctatcac
      301 cacgctcata ggttttgcca atttcaataa tagcaaaacc atcagcccaa acggctgacg
      361 ataatacgcc actgctttgg ttatggtatt tgcttaatac ccattcacct tgttcatttt
      421 gtactaaacg aacacgtaaa tactcctgac ggccttgcgc tttttttatc gaaaaatcga
      481 taggcatcgc caccacactt ggtttaactt tatgacgttg accttgataa taagctaaaa
      541 acgtttgagc catgacttta aaggtaacaa acaccgattg tggattacca ggtaaaccaa
      601 taaagggcgt tgtaccaata gagccaaatg ccaagggctt gcctggtttg agcgcgactt
      661 tccacaaatc taattggcca atttgttgta atgcagcttt aacgtgatct tcttcaccaa
      721 cagacacacc gcctgtagtc atcaccacat cagcttgctg agcagcattg gccaacgcat
      781 taatcgtatc tggcaaatta tcggcaatca caccaccatc aatggcttga taaccttgtt
      841 cggttaataa agcacctaat aatgggctat tactattata aatctgccct acttttagcg
      901 tttgctctgc ttgggctaac tcatcacccg tacaaaaata gacaaccttt aaacgctgat
      961 aaaccttgac cttagcctta ccaatggttg ccaacaaacc aattgcggct ggcgtaagtt
     1021 tttcgccctg acgcaaaatg gtaacgccac aagcaatatc ttgaccttgt ggccgaatat
     1081 tttcttgggc atcaggtcgc tgtaaaactt ctacaaggcc atctgctgtg agttgggtgt
     1141 tttcttgcat taccaccgca ttagcaccat caggaatgac tgcacctgta aaaatacggg
     1201 cggctgtgcc tcgttgtaat ggttgtggcg cactgcctgc ggcaatacgt tggctgatgg
     1261 ctaaaatagt tgtgtgttgt aaatcatcgg tataaacagc ataaccatcc atcgccgaat
     1321 tagcttgagg aggtacatca atcgtagcaa cgacatcttc ggctaacaca cgacctaaag
     1381 catcacttaa taaagtttgc tctatgacgg gagttttttg gctcgcggct tgttgcaaac
     1441 gttgtaaaac ctcatcaacc gacattaaac cgtgactagg acaactcatt agccacgact
     1501 cccacacaac tcagcttttt tcaggttagg gacaaagttg caaggacgat gctgattatc
     1561 aagttgttgt gacaaaatat tgatccatgc tgttttgcac gcattggttg aacccggcat
     1621 acaaaaaatc accgtgttat tggccatgcc tgccaaagca cgcgattgta aggtgcttgt
     1681 gccaatttct atcatcgaat aatgacggaa aatctcacca aaaccttcga tatgtttatc
     1741 aaacaacaca ttcactgctt cgggtgtgct gtcgcgccct gaaaaacctg taccacctgt
     1801 caccaaaatc gcttgtacat ttggactagc aatccatgcc gaaataatgg cgcgaatttg
     1861 ataaatatca tctttaacaa tttgtctgtc tgctaaaaga tggcctgctc ccgttaaggc
     1921 atcaatcaaa tattgaccag aggtatcggt ttctaaatta cgggtatctg atacggttaa
     1981 tacggcaatt tgtaatggca caaaaggttt attgattgcg gaatgactca taataactcc
     2041 taaaatgtgt aaagaattgt ctcccaacaa tttagggagt gtataataat acttcaactt
     2101 tgctcaggga acacagtggg ttgagcgaag tcgaaacgac ctacaatatt atccgcctgt
     2161 catattcata aaacgcacaa tttgcggctc atcgtttaaa tcaaaatgat gcttttcggg
     2221 ctttaagggt aatgcgttaa ataaagtatc aattaatggt tgatcgtcat gaggaaagtc
     2281 gcgtaatggc gcttttagat cggcagaatg ttcattgcct aaacataata acaaacgccc
     2341 ttctgccgtg actcgcactc gattacatga gccacaaaaa ttgtgactat gcggtgaaat
     2401 aaaaccaatt cgactctgac tatcgctcat atgataataa cgagctggcc cacctgtgga
     2461 ttcagaaaca gcagttaacg gataaaattg atctatgcta tggcgaactt ggtcattgct
     2521 gatataggtt tcagcacgtt gatgctcagt aatgacacct aatggcattt cttcaataaa
     2581 ggctatatct aattgatttt ggcgaataaa attgactaag tctaaaatct catcgtcatt
     2641 tttgcctttt aaaatcacac tatttaattt aatgtgttga aaacctgcct gttgggcagc
     2701 ttcaatacct gcaagcactt cggataattt acctgtgcga gtaatttgac taaaacgttg
     2761 ttcttgcaaa ctatctaagc tgatattaat ccgtgataaa cctgcctctt ttaaaggctg
     2821 tgctaagcgc gttagctgtg aaccatttgt ggttaaggtt aattctttta agccttgtaa
     2881 acttgctagc ttttcaacta gccaaactat attttgtcta actaagggtt caccgccagt
     2941 taaacgaatt ttgctaaccc ctaaactgac taaaacgcgt cctacacgat aaagctcctc
     3001 tagcgtcaaa atttgctgac gtggcaaaaa taccatgtct tcgcccatac aatagacaca
     3061 gcgaaaatca caacgatcag tcaccgataa acgaacataa gtaatttgac gaccaaaacc
     3121 gtcaattaag gcaggattag acatgaaaaa ggtataagcc tgtaaataaa aactagaata
     3181 ttgttttact ctgttttata aaaaaatact attggcttaa agtacagttt taaactggcc
     3241 tttgagtcaa tagtatccat ccaagataag ctaataatag tgctaagcca acaaccgatt
     3301 taaagtttca aaagtatcgt ttgacagtga ttgctcgcgc agtttttgca atgcagcttg
     3361 tgcttgttgt ttaagtgtag ggcgtaaacg ctgccatcgg ctaaatgctc ccaataagcg
     3421 cgaggcaatt tgcggattga gcttatctaa ttcggcaatt ttttgggtaa caaaacgata
     3481 accatcgcca ttttgttgat gaaataaaac aggattgcta tttgcaaact gtgccagtac
     3541 agaacgtagg cgatttgggt tggttaagct aaatgcttga tgctcggtga gagctttaat
     3601 atcactcaaa cttgcttgtg ggttggtagc ttgtaccgca aaccagctat caatcactaa
     3661 ggcttcttgc tcaaaacgcc aataaaaatc ctgtaaacac tcagccgttt gcggggcttg
     3721 gctatgcact aagccacgca aggcacacat gcgctctgtc atattttgcg cttgttgata
     3781 ttgttgtacg gctaattgtt gcggtatttt attatcaacg gtggccaata agtagcgcaa
     3841 acacacatcg cgccaagcgc gtatggcaat agcttgggca ttgtaaatat agtcactatc
     3901 attggcattt ttagcatatt gctcgctaaa aaaacattct agcgcattgg ctatttcggc
     3961 ttgcaacatg ttacgggctt gatgaatacg cgcaggataa ataacggctg ttttttcggc
     4021 caaataattt tcgataggca aacttaacaa tttggctgct aaggcagggt cttgctcggc
     4081 taattctggc aacaattcag ctaaggccgt taatacatta tcaatattgg tattatcaaa
     4141 ggcatctttt tgtgcattga tattggcaat catggttagc aacatacgct caaataaact
     4201 ttgagccgac aaccaacggt taaaaccatt atcatcgtac tgcatcaaaa acaataattc
     4261 ttggtcagtg gtctgatagt ccaaaataac aggcgccgaa aaatcacgta ataacgaggg
     4321 aacaacgcct cgttcaatac ctgtaaaaac ccattgttgc tcagctgcat cagcaattaa
     4381 cacccgctct tttcccattg cttgggtttc gtgtgccagt tttagggcaa tagctcggcc
     4441 agtttgtgca tcaaataatg ccaatttaac aggtatgggt aacaattgtg gctcatcaaa
     4501 gccttgttta tgcggtgttt tttgactcac attaagtgta tacgttttag attttgcgtc
     4561 atattgacca ttaaccgtta atacaggtgt acacggctgt ttgtaccatt gcataaacgc
     4621 gcttaaatcg gtgttatttg cttgtgcgag ggcatttaca aaatcttcta ctgttactgc
     4681 ttggccatca tggcgactaa aatataaatc tgtacctttt ctaaacgcgt ctgcgcctaa
     4741 tacggtatgt agcattctga cgatttccgc gcctttctca tacaccgttg cggtataaaa
     4801 attattgatt tcaataaatg attcggggcg gacaggatgg gctaaaggac tggcatcttc
     4861 ggcaaattgc acattacgca ataccgcgac atcttcaata cgttgcacac tggcagagag
     4921 ttggtcggca gaaaattgtt ggtctctaaa aacggtaaaa ccttctttta gactgagttg
     4981 aaaccaatct cgacaagtga cccgattacc gctccaatta tgaaagtatt catgggcaac
     5041 cacggattca acacgctgaa aacctaaatc agtggtggtt tcgggatggg ctaacacaca
     5101 cgaggtatta aagatattta agcctttgtt ttccattgcc cccatattaa agtggctaac
     5161 ggcaacaatc ataaaaatat ctaaatcata ttcacgacca tagacttgct catcccaacg
     5221 cattgacgct tttaaagact ccattgcatg gtgacattgg cgcaaatcgt gcggttctac
     5281 ataaattttt agggcaacct cgcgacccgt catggtggta aagctatctt ctaaacaggc
     5341 taaatcacct gccaccatcg caaataaata gcttggtttt ttgaaagggt cttgccatgt
     5401 ggcaaaatgt ctgccctcac ctgcatcacc ttgggcaatc aaattaccgt tagctaataa
     5461 agtcggatat tggtttttat cggcagtaat ggtggtagta aaagacgata aaacatctgg
     5521 acggtctaaa taatagctaa tttttctaaa gccttctggc tcacattggg tacaaaacat
     5581 ccctttcgac agatacaaac cttctaaggc caaattttta gcaggataaa tttttaccac
     5641 actttgtagg ctaaacggct gattaggcac gttgtgaatg actaactctt caccttgtag
     5701 ttgataatca tcaattgata ataattgatt atccaaatgc aagttttgta gttctaagtc
     5761 aacaccatgt aacaccaacg cttgattttt ttgttggggg gtcaaattta gggttgatgt
     5821 gactaaggta taatcgttaa aaatatcgac ctgtaaatta acttgctcaa tgctaaataa
     5881 cggggcttga taatctttta aataaacggt ttttgggtta gtcattataa atctctaaaa
     5941 tatta
//
LOCUS       DAOQMB010000112         5891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5900_length_5891_cov_12.839616, whole genome shotgun sequence.
ACCESSION   DAOQMB010000112 DAOQMB010000000
VERSION     DAOQMB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5900_length_5891_cov_12.839616"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /gene="rtcA"
                     /locus_tag="PLY05_05345"
     CDS             complement(<1..722)
                     /gene="rtcA"
                     /locus_tag="PLY05_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNC02764.1"
                     /translation="MFWWLSVVVVVERSRNHDDEYKGFFMITIDGSYGEGGGQMLRSA
                     LALSMITGQAFTMHSIRAKRSKKGLLRQHLTAVKAAQQICSAEVTGAELNSLQLTFIP
                     QAIKHGNYKFEIGTAGSTTLVLQAILPALLFADDISTVTITGGTHNQSAPPADFLQLA
                     FLPELAKMGANVELNIRRHGFFPAGGGEINVTVHPCKQLKPLVLIERGQEQQRFATSV
                     ISNLPSHIAERELASLQTKLGLT"
     gene            complement(766..1986)
                     /locus_tag="PLY05_05350"
     CDS             complement(766..1986)
                     /locus_tag="PLY05_05350"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNC02765.1"
                     /translation="MKTGNYDVLQNDKSSPIKMWTQGVPVEDAAKKQLLNAANLPFIY
                     KWIAAMPDVHLGKGATIGSVIPTKGAIIPAAVGVDIGCGMIAVKTSLHATDLPDNLAP
                     LRSAIEKAVPHGKTFGKRDEGAWHNPPAEADAAWSVLADDFKKITDKYPRLKNTNNRN
                     HIGTLGTGNHFIEVCLDENQQVWFMLHSGSRGVGNAIGEHFIALAKRDMERWFIHLPD
                     KDLAYFAEQTEHFDDYCYAVEWAQRFAKANRELMMQNVIKAARGIISKPFKADMLAVN
                     CHHNYVSREMHYGENVLVTRKGAVSARKDELGIIPGSMGACSFIVRGLGNEESFQSCS
                     HGAGRTMSRAEAKRRFTIEDHAKATEGVECRKDQEVVDETPAAYKPIEAVMHAQRDLV
                     EVVHTLKQVVCVKG"
     assembly_gap    2608..2707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2708..4242)
                     /locus_tag="PLY05_05355"
     CDS             complement(<2708..4242)
                     /locus_tag="PLY05_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HNC02766.1"
                     /translation="MANANLFKSMVGKLLPSTNTKNEAGSNAYAFTPRHALAQYAMTG
                     CLNHTFYSSAEMQLEKVLTTAQEIDDEFLAKLAIYSREQGAMKDMPAVLLAILAARQS
                     AYFAVAFARVVNNGKMLRNFVQIVRSGVTGRKSLGSRPKRLVQTWLNEASTPKLLNAA
                     VGNDPSLADVVKMVHPQPKNAEQAAVFAWLIGKPFDVNALPKVIQDYELVKRGVSIDL
                     PDVPFQMLTHLTLNTEQWTNIGKNMGWQALRINLNTLLRHGVFNDAKMVNWVADRLRD
                     KESIRKSNAFPYQLMMAYQAAKQEMPAPIVDALQDALEVALSNVPDLQGKKVVVCPDV
                     SGSMQSVVTGYRKGATSSVRCIDVAGLVSAAMLRNNPQTVVLPFENKVVDIRLNSRDS
                     VLTNAKRLASIGGGGTDCSAPLTWLNNQKSAVDVVIFVSDNESWLRNYAYGASETMRQ
                     WAIIKQRNPKAKLICIDIQPSASTQALDGEDVLNIGGFSDEVFKLIGLFANNQLSANH
                     WVEV"
     gene            4660..>5891
                     /gene="rtcR"
                     /locus_tag="PLY05_05360"
     CDS             4660..>5891
                     /gene="rtcR"
                     /locus_tag="PLY05_05360"
                     /inference="COORDINATES: protein motif:HMM:NF038308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR"
                     /protein_id="HNC02767.1"
                     /translation="MKTIVFSFLGSQLDAAKGRGRWEKWRPNVALCQHDDFIIDKLVL
                     FYNEQYLDLLNVVIDDIAFISPETVVEPYLMPLENPWDFQEVYGALYEYARAYQFDID
                     KHQYLVHITTGTHVAQICLFLLTETRYFPAKILQSSPPRKQQNGESGRLDIIDLDLSR
                     YNLLASRFADERLEIQNHLKSGIATKNPTFNVMIAEIEQVALRSRSPVLLLGATGVGK
                     SFLARRVYELKKQRHQLRGEFVEVNCATLRGDSAMSNLFGHAKGAFTGAVSARAGLLR
                     SADGGLLFLDEIGELGADEQAMLLKAIEEKRFYPVGADKEVQSDFQLIAGTCRDLAEE
                     VRLGNFREDLYARLNMWTFHLPPLSERREDIEPNIEFELARFAREQGREVRFNKEARQ
                     DYLAYALQPKALWSGNFR"
BASE COUNT         1638 a         1212 c         1207 g         1734 t
ORIGIN      
        1 gttaaaccca atttagtttg taaactggct agttcacgtt cagcaatatg gcttggcaaa
       61 ttactgataa cactcgttgc aaaacgttgt tgttcttggc cacgttcaat taatactaaa
      121 ggttttaatt gtttacaagg atgaacagtg acattaatct cgccgccgcc tgcgggaaag
      181 aagccatgtc ggcgaatgtt taactcaacg tttgctccca tttttgctaa ttcaggcaaa
      241 aaagctaact gtaaaaaatc agcaggtggt gcagattggt tgtgtgtgcc tcctgtaatg
      301 gtaacggtgc taatatcatc ggcaaataat aaagcaggca atatggcttg taggacgaga
      361 gtggtgctac ctgctgtgcc aatctcaaat ttatagttgc cgtgtttaat cgcttgcgga
      421 ataaaggtga gttgtaggct gttgagttct gcacctgtga cttcggcaga gcaaatttgt
      481 tgtgctgctt ttaccgccgt taaatgttgg cgtaataaac cttttttact acgtttggca
      541 cgaatgctgt gcatggtaaa ggcttggcca gttatcatgc ttaaggcaag ggcagagcgg
      601 agcatttgac caccgccttc accataagaa ccgtcaattg ttatcataaa aaatcctttg
      661 tattcatcat catggtttcg actacgctca accaccacga ctacgctcaa ccaccaaaac
      721 atcctccgct caatgagtga atgctccctg agcggagtcg aagggttacc cctttacaca
      781 aaccacttgt tttaaggtat gaaccacttc taccaaatca cgttgagcgt gcataactgc
      841 ctcaatcggt ttataagcag caggggtttc atcgaccacc tcttgatctt tacgacactc
      901 cacgccttca gtggctttgg catgatcctc tatggtaaag cgtcgtttgg cttcagcgcg
      961 tgacatggtt cgtcctgcac cgtgtgaaca gctctgaaaa ctttcttcat ttcctaagcc
     1021 acggacaata aacgagcaag cacccatact ccctggaata atccctaatt catctttgcg
     1081 cgcacttact gcacctttac gcgtcactaa gacgttttca ccataatgca tttcacggga
     1141 tacatagtta tgatgacagt tcaccgctaa catatccgct ttaaacggtt tgctaataat
     1201 accacgagcc gctttaatca cattctgcat cattaactca cggttagcct tagcaaaacg
     1261 ttgcgcccat tcaaccgcat aacaataatc atcaaaatgt tcggtttgtt cagcaaaata
     1321 ggctaggtct ttatcgggta aatggataaa ccaacgttcc atatcgcgtt tggctaaggc
     1381 aataaaatgc tcaccaatcg catttcccac accgcgtgat ccactatgca acataaacca
     1441 gacttgctga ttttcatcta aacacacttc aataaagtga ttacctgttc ctaatgtccc
     1501 aatatgatta cggttattgg tgttttttaa gcgtggatat ttatcggtga tttttttaaa
     1561 atcatcggct aacactgacc atgccgcatc cgcttcagca ggtggattat gccatgcacc
     1621 ttcatcacgt ttaccaaagg tttttccgtg tggtactgct ttctcgatag cagagcgtaa
     1681 gggggctaaa ttatcgggca aatctgtggc atgtaaggaa gttttaaccg caatcatccc
     1741 acaaccaata tccacaccca ccgccgcagg aataattgcc cctttggttg gaatcacact
     1801 accaatggtt gcgcctttac ctaaatgtac atcaggcata gccgcaatcc atttataaat
     1861 aaaaggtaag tttgctgcat ttaacagttg tttttttgcg gcatcttcta cgggcacacc
     1921 ttgtgtccac attttaatcg gtgagctttt atcgttttgt aatacgtcat agttaccagt
     1981 tttcatgttg atttcctttt taaataaaat agcgactaag ggtaatgaga tgtttttgcc
     2041 attttcgcca cttgctaagc aagtcgggat tcgaacccga aaagagaggg atttttagtc
     2101 ccttgcgtat gtaatcccat caaagtattc gctataactc attaactaag gacataagtc
     2161 gcttagcata aacatgactt acgcatttga tctaaattat cgcgtaagtc ctaaaatagg
     2221 tgcagcaacg agttttatgt gaaattttta cactaaccaa atgtaattcc acaggcattt
     2281 gctgcaaatt gggagctaat gactaggggt atgagataga tttacagtct atgtaatccc
     2341 attagcattc attagcaaaa ctaagtgtga tgactaaagt tggtgagata cgtgtaccaa
     2401 aggcacactg attaccagtc agatgtattc ccacagagca ttcatcacat atttaattaa
     2461 ctagcaacta ggggtatgaa acattcctgc tctacctact gagctacagc cccataacgt
     2521 gaatattttg gtggagctga cgggagtcga acccgcgaca cggtcgtttt cagcgatgta
     2581 gttccataag catttgctag ttaaactnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnacc tcaacccaat gattggcact caattgattg ttggcgaata gcccaatcaa
     2761 cttaaatact tcatcactaa agccgccaat gtttaaaaca tcctcaccat ctaaagcttg
     2821 tgtgctggca gaaggttgga tatcaataca aatcaatttg gcttttggat tgcgttgttt
     2881 gatgatggcc cactgacgca tggtttcgct tgccccataa gcataattgc gtagccaaga
     2941 ctcgttatca gagacaaaaa tcaccacatc aaccgccgat ttttggttgt ttaaccaagt
     3001 caacggtgcg ctacaatctg tgccaccacc accaatactt gccaaacgtt tggcattggt
     3061 tagcacgcta tcacggctat tcaaacgtat gtctaccacc ttgttttcaa aaggcaacac
     3121 aacggtttgt gggttattac gcaacattgc cgcagagacc aaacccgcca catcaataca
     3181 acgtacagaa ctggttgcgc ctttgcgata acctgtgact accgattgca ttgaacctga
     3241 cacatcagga caaaccacaa ctttcttgcc ttgcaagtct gggacattgc ttaaagcaac
     3301 ttccaacgca tcttgcaaag catcgacaat gggtgcaggc atctcttgtt tagccgcttg
     3361 gtaagccatc atcagttgat aggggaaagc attgcttttg cgaatgctct ctttatcacg
     3421 caaacgatca gcgacccaat tcaccatctt cgcatcatta aacacgccat gacgtagcag
     3481 ggtgtttaga ttgatacgca aggcttgcca acccatattt ttgccaatat ttgtccattg
     3541 ctcagtgttc aaggtcaaat gagtcaacat ttgaaaaggc acatcaggca aatcgatgct
     3601 gacaccacgc ttaactaatt cataatcttg aattacttta ggcaaggcat tgacatcaaa
     3661 aggcttgcca attaaccaag caaaaacagc ggcttgctct gcatttttag gttgagggtg
     3721 aaccatcttc accacatcag ccaacgaagg atcattaccc acagcagcat taagcagttt
     3781 tggggtagag gcttcgttta accatgtttg taccaaacgt tttgggcgag aacccaagga
     3841 ttttcgacca gttacgccag agcgcacgat ttgtacaaag ttacgcaaca tcttgccgtt
     3901 gttaaccaca cgagcaaaag ccactgcaaa ataagcactt tgacgtgctg ccaagatcgc
     3961 caacaacacg gctggcatat ctttcattgc cccttgttcg cggctataaa tcgccaattt
     4021 agccaagaat tcgtcgtcaa tttcttgcgc tgtggttaat accttttcca actgcatctc
     4081 ggcagagcta taaaaggtat gattcaaaca acctgtcatg gcatattgtg caagggcatg
     4141 acgaggggta aaagcataag cattgctgcc tgcttcattt tttgtattgg ttgatggcaa
     4201 caactttcca accattgatt taaacaagtt tgcatttgcc atgttaaaaa ctcctgtttt
     4261 tatagacttc gtttatttaa gtcattttga atttttcgat tttcgcttgc tgtataaatt
     4321 gcatggcatg tgccaaattg aaaaaattat caaaaaataa tttataagtt attgaattgt
     4381 aatgattaaa aattttattt tttctttttt aagttttttg gtgtggttta aaaatttaat
     4441 ttttatatat ttatagatag tgtattataa aaatatataa atctttatga tggttgagcg
     4501 tagtcgaaac cattaagatt agatacagtc ggtagcctgt gtggagtgta acgtaacaca
     4561 ggattactca atttaaagcg ttgtttttcc tgtatttcgc tacgctgcat acaggctaca
     4621 aatatttaac gtaactctga ggaatggtat aaaaaagcca tgaaaaccat tgtttttagt
     4681 tttttaggtt cacaattaga tgcagccaaa ggtcgtggac ggtgggagaa atggcggcca
     4741 aatgtggcct tatgtcaaca tgatgatttt attattgata aattggtgtt gttttataac
     4801 gagcaatatc tcgatttatt aaacgtggtg attgatgata tcgcctttat ttcgccagaa
     4861 accgtggttg aaccctattt aatgccgttg gagaatcctt gggattttca agaagtgtat
     4921 ggcgcattgt atgagtatgc gcgtgcttat caatttgata ttgataaaca tcagtatttg
     4981 gtgcacatca ccacaggcac acatgtcgcg caaatttgtt tgtttttgct gacggaaacg
     5041 cgttatttcc ctgccaaaat tttgcaaagc tcaccaccac gtaaacaaca aaatggcgaa
     5101 agtggtcgtt tagatattat tgatttggat ttgtcgcgct ataacttatt agccagccgt
     5161 tttgccgatg aacgtttaga aatccaaaat cacttaaaat cgggtattgc caccaaaaat
     5221 ccgactttta atgtgatgat tgccgaaatt gaacaagtgg cattacgttc acgtagccct
     5281 gtattgttgt tgggggcaac gggggttggt aagtcatttt tggcgcggcg tgtctatgag
     5341 ttaaaaaagc agcgtcatca gttgcgcggt gaatttgttg aggtcaattg tgcgactttg
     5401 cgaggtgata gtgccatgtc taacttgttt ggtcatgcca aaggagcttt tacaggggct
     5461 gtgagcgcaa gggcgggttt gttgcgtagt gctgatggcg gcttgttatt tttggatgaa
     5521 attggtgaat tgggtgcaga cgaacaagcg atgttgttaa aagccattga agaaaaacgt
     5581 ttttatcctg ttggggcaga taaagaagta caaagtgatt ttcaattaat tgcaggtacg
     5641 tgtcgagatt tggcggaaga agtgcgttta ggcaatttta gagaagattt gtatgcacga
     5701 ttgaatatgt ggacgtttca tttgccacct ttaagcgagc gtcgtgaaga tattgagcca
     5761 aacatcgagt ttgaattggc acgttttgcc cgtgaacaag gccgagaagt gcgttttaac
     5821 aaagaagcgc ggcaagatta tttagcttat gccttacagc ccaaagcctt gtggtcggga
     5881 aactttaggg a
//
LOCUS       DAOQMB010000113         5869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_5936_length_5869_cov_16.859649, whole genome shotgun sequence.
ACCESSION   DAOQMB010000113 DAOQMB010000000
VERSION     DAOQMB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5936_length_5869_cov_16.859649"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLY05_05365"
     CDS             complement(<1..485)
                     /locus_tag="PLY05_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02768.1"
                     /translation="MNNNKDTSSISFTAHYTGYVWYKNKLSHRAFATKQGFAFYQLLR
                     PIEILAKFFIGSDIKTTLLQRHQLIDRELTQLILLHPDLQILELACGLSPRGWRINQK
                     YPYIPYVEADLVGMASQKQALLEHLDALNDTHKVRTCNILIENHEDSLESVLQREFDF
                     N"
     gene            complement(493..762)
                     /locus_tag="PLY05_05370"
     CDS             complement(493..762)
                     /locus_tag="PLY05_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02769.1"
                     /translation="MKPLSDEQFKALSHLVYRDLILDTLGNLSLGLGLYLVFSKEAAH
                     WPTWLLSPEAKAIFIATGLINLRFIAIRFRRLQAWQMARQQRKQR"
     gene            complement(746..1765)
                     /gene="holA"
                     /locus_tag="PLY05_05375"
     CDS             complement(746..1765)
                     /gene="holA"
                     /locus_tag="PLY05_05375"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNC02770.1"
                     /translation="MKYDYWGLLKHLKNPLRTIYVLHGDETLIQQEALIALRQVAKQQ
                     GFLEHERHDVDKHYDWSMAFADSNALSLFSDKKLIEIHSNKPDAAGAKVIEAYLQSPP
                     EDNVVILVLPKIDAAAQKSKWFTACEDHGITVSCAPLTDYQFKDWIVQRCQSHKVQLD
                     NSALTWLCQQTEGNLLAAHQEVTKLALLFGSEKISLEQLEQSISDSSRFDLFDLSACA
                     LAGDAQKTAKILFSLQAEGQAESLILWVLAKEVRMLTNLFEGTQQGVSFSNLCQQLGI
                     WTKQQNGYQLALRRLNHKRLSRLNAELLAADKAIKGQNNDNAWDILLRLSLGLAGVTL
                     FETAV"
     gene            complement(1762..2265)
                     /gene="lptE"
                     /locus_tag="PLY05_05380"
     CDS             complement(1762..2265)
                     /gene="lptE"
                     /locus_tag="PLY05_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNC02771.1"
                     /translation="MKFGNSVLCSLMIISLTACGFHLRGSYHIPPQLQQLNVDLSTAS
                     PLSSPLNQRLQQSGIQLNQGEYRLVILEDKLSKQTTNTDSRAKAAEYSLYYQVRYVLQ
                     NTEKQNVSEERKLLLRRSYQYDTTAIVGKTAEEETLIKELYEEAATQITRQLSGFSHL
                     STTKTTP"
     gene            complement(2398..4992)
                     /gene="leuS"
                     /locus_tag="PLY05_05385"
                     /pseudo
     CDS             complement(2398..4992)
                     /gene="leuS"
                     /locus_tag="PLY05_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
     gene            5288..5758
                     /locus_tag="PLY05_05390"
     CDS             5288..5758
                     /locus_tag="PLY05_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNC02772.1"
                     /translation="MDQQYLELLQAMPIFGGVSQQALSFLLTRCPEIEIAQNAYFFKE
                     GEQADSLFILERGTALVLKGRDIKLRYLVRGDCFGEMSLIDLNPRSASVRAIEPCIAL
                     ELSRQSLFALYEYDLEQFVIIQMNLSREVSRRLRALDERLMSLDTELLKLINVL"
BASE COUNT         1677 a         1162 c         1237 g         1793 t
ORIGIN      
        1 ttgttaaaat caaactcacg ttgcaaaact gattcgaggc tatcttcgtg gttttcaatt
       61 aaaatattac aggtacgcac tttatgcgtg tcattaagtg catctaaatg ctctaataac
      121 gcttgctttt gtgaagccat ccctaccaaa tcagcttcta cataaggaat gtaaggatat
      181 ttttggttga tacgccagcc gcgtggtgat aaaccgcaag ccagctctaa aatttgtaaa
      241 tctggatgca ataaaattaa ttgagtgagt tctcggtcaa ttaattgatg acgctgtaac
      301 agcgtggtct tgatatcaga accaataaaa aatttggcca aaatttcaat agggcgtaag
      361 agttgataaa aagcaaaccc ttgtttagta gcaaaggcac gatgtgagag tttattttta
      421 taccaaacat agcctgtata atgagcggta aacgaaatac ttgaagtgtc tttattatta
      481 ttcatgatgt tcctaacgct gtttacgttg ttgacgagcc atttgccatg cttgtaaacg
      541 acgaaaacga atggcgataa accgtaaatt aatcagtccc gttgcaataa aaatcgcttt
      601 agcttcgggc gataataacc atgttggcca atgagccgct tctttactaa aaaccaaata
      661 taaacctagg cctaatgaga gattgcctaa agtgtctaaa attaaatcac gatagacaag
      721 gtgtgataat gccttaaatt gttcatcaga cagcggtttc aaagagagta actccagcaa
      781 gaccaagact taaacgtaat agtatatccc aagcattgtc attgttttga cctttgatgg
      841 ctttatctgc ggctaatagc tcagcattta ggcggcttaa acgtttgtga ttcaatcgtc
      901 taagtgcaag ttgataacca ttttgttgtt tcgtccagat gcctaattgt tgacataaat
      961 ttgaaaagct aacaccttgc tgcgtgcctt caaataaatt ggtgagcatg cgtacttctt
     1021 tggccagtac ccataaaatt aagctttctg cttggccttc ggcttgtaaa ctaaataaaa
     1081 tctttgccgt tttttgcgcg tcacctgcta aagcacaggc acttaaatca aataaatcaa
     1141 aacgactact gtcactgata ctttgttcga gttgttcgag gctaattttt tcactgccaa
     1201 ataataatgc cagtttggtg acttcttgat gggctgccaa caaattacct tcggtttgtt
     1261 ggcataacca tgttaaggca gaattatcaa gttgtacttt atggctttgg cagcgttgta
     1321 caatccagtc tttaaattga tagtcggtga gtggtgcgca agaaacggta atgccatgat
     1381 cttcgcaagc ggtaaaccat ttgctttttt gggcagccgc atcaattttg ggtaaaacca
     1441 gaatgaccac attatcttcg ggtggtgatt gaagataagc ttcaatcact tttgcgcctg
     1501 cagcatcggg tttgttgcta tgaatttcaa tcagtttttt atctgaaaat aacgagaggg
     1561 cattactatc tgcaaaggcc attgaccaat cataatgctt atcaacatca tggcgttcat
     1621 gctctaaaaa accttgttgt ttggcaactt ggcgaagggc aatcagtgct tcttgttgaa
     1681 ttaatgtttc atcaccatgc aacacataaa tcgtccgtaa aggatttttt agatgtttta
     1741 ataagcccca atagtcgtat ttcatggtgt ggtcttggtg gtggaaagat gactaaagcc
     1801 actgagttga cgggtgattt gggtggctgc ctcttcatat aattctttaa ttaaggtttc
     1861 ttcttcagcg gttttaccaa caatggctgt ggtgtcatat tgataactac gccgtaatag
     1921 cagcttacgt tcttcagaaa cgttttgttt ttcggtattt tgtagcacat aacgtacttg
     1981 ataatacaag ctatattcag ccgctttggc acgactatcg gtgttagttg tttgtttact
     2041 gagtttgtct tctaagatca ctaaacgata ttcaccttga ttaagttgaa tacctgattg
     2101 ttgtaagcgt tggttaagcg ggctgcttaa tgggctggca gtgcttaagt cgacgttgag
     2161 ttgttgtagt tgtggaggaa tatgataact gccacgcaag tgaaaaccac aggccgttag
     2221 gctgatgatc atcaaggaac aaagcacaga attaccaaat ttcattcaca acatactcta
     2281 tggctgtagg atgggttgat gcaggaaacc catcattggc agattgatgg gtctcgtacc
     2341 tcgacccatc ctacaagaga cgaggtatta ttaagcagtg ggttacacca caatattgac
     2401 taacttacct ttcaccacaa tcactttttt cggtggagtt tctaaaaagc gttggatttc
     2461 aggtttagcc aaagccagtt gctcaactgt ggcattatct gcatcaacag caacttcgat
     2521 tttgccgcgt aacttgccgt tgacttgaat cattaattca atgctagaac gtgttaacgc
     2581 attttgatca accgttggcc atgcggcatt gacaatatcg ctatggccta atgattgcca
     2641 taatttttcg gcaatatgtg gcacaatcgg cgacaaaatc aaaatagcgg cttctaaggc
     2701 ttcacgctca acagctaagg cttgaccttc tttggcatct aatttttgca cactgtttaa
     2761 caactccata atcgcagcaa tggcggtgtt aaaggtaaaa cggcgtgcga tgtcatcagt
     2821 ggttttgcca atcgtttcgt gggttttacg gcgtaaatca cggctattat tgtctaagtt
     2881 ggcaacatct aaggcaggga taaagccttt ttgttgatgt aaatagacac tgcgccacaa
     2941 acgttttaag aaacggtgcg cgccttcaac gccagcatct gaccactcta aggattgatc
     3001 gggtggtgaa gcaaacatca taaacaaacg ggcagtatct gcaccgtatt ggtcaatcaa
     3061 tgcttgtgga tcaacaccat tgtttttaga ttttgacatt ttttccatgc cgccaatcaa
     3121 cacaggctga ttgtcagtgg tcaaaatagc gttaatcgca cgacctttgt catctttttc
     3181 taaagcaaca tcagcaggat taaaccaatc ttttttacca tcaggcattt cgcgataata
     3241 ggtatctgct aacaccatgc cttgggttaa taaacgttta aacggctcat caccttgtac
     3301 caaaccttca tcacgcatta acttgtgaaa gaagcgggca tataacaagt gcaaaattgc
     3361 gtgttcaatc ccaccaatat attggtcaac aggcaaccat gtgtttgcgg cttgtgggtc
     3421 taccatgccc gtttcgcatt gtggtgacgc ataacgcgca taataccaac tcgattcaac
     3481 aaaggtgtcc atggtgtcag tttcgcgagt ggctgcacca ccacagcttg gacaagtggt
     3541 ttggtaaaac tcaggtaagc gttttaaggg tgaagcaccg ccatcaggca cgacatcttc
     3601 gggtaaaacc acaggtaatt gatgatctgg aacaggcaca tcaccacagc ttgggcaatg
     3661 aatgatggga attggacaac cccaataacg ttgacgagaa atcccccaat cacgtaagcg
     3721 gaattgagtt ttgcgggtgc catgttgtgt cgcttctaaa tcggcaacga tggcatcaaa
     3781 agcagccgta aaacttaaac cgtcatactt gccattattg atggagcgaa tgctgtcatc
     3841 tttattggca taccaatctt gccaagtgtc aacgttaaac tctgcacctg ttgtcgattg
     3901 atagacttgt ttaatggtta attgatattt atgggcaaat tcaaaatcgc gttcatcatg
     3961 ggcaggaaca gccatgactg cgccttcgcc ataaccccat aatacataat tggcaatcca
     4021 tacgggtaat ttgtcacccg ttaatggatg cgtgacataa aagcccgttg caacgccttt
     4081 tttctccatc gttgccatat ctgcttcggc cacagagcct gctttacatt cggcaataaa
     4141 gtcttgtaac gtgggatttt tttcagccgc taaagttgct aaaggatgtt cagccgctac
     4201 tgcgacatag gttgcaccca ttaaagtgtc agggcgtgtc gagtaaactt ttaattgacc
     4261 agtttgacca atgctgtctt ggtcataatc aaaaataatg tctgcaccaa agcttttgcc
     4321 aatccagttg cgttgcatgg ttttgacttg ttctggccag ccgtctaatt ggtctaaatc
     4381 ggttaataat tggtcagcat aggcggtgat tttaaagtaa tacatgggga tttcgcgttt
     4441 ttcaacgact gcacccgaac gccagccttt gccatcaatt acttgttcgt tggctaatac
     4501 ggtattgtca acaggatccc agttcacaat ccctgatttt ttataaataa tgcctttttg
     4561 aaataaacgg gtgaataacc attgttccca gcgataatag tctggtttac aagtggttac
     4621 ttcacgttgc caatcaatcg ccaagcccaa agactgtaat tgctttttca tataagcaat
     4681 gttgctgtat gtccatgcgg caggtggcac attatttttc atcgctgcat tttcagcagg
     4741 cagaccaaag gcatcccaac ccatcggttg tagcacgtta aagccttgca tttttttgaa
     4801 gcgggctaat acatcgccaa tggtatagtt acgcacgtgt cccatgtgta gcttacctga
     4861 tggataagga aacatggata aacagtaaaa tttgggtttg tcgctgttat cattggcggc
     4921 aaaggcgttg ttgtctgccc attgcgcttg tacggtgggt tcgatgtctt ggggtaaata
     4981 attttcttgc atggcaattc gtttgctgaa taaaaagagg taaagaggaa ttcctctcgc
     5041 tattttccct gaataaagct gaaagttaca ggcagggagc gagaaatcct aataaagccg
     5101 aatagcataa cctagctgat gctatttaag taagtgtttc gcggcaatat catcaaaaaa
     5161 atctaatcat taaaaaatct tctacactgt aaataaacaa gatttagagt acaggacagg
     5221 cgtattgtct cacaattaac gcataaatcc tgattgtcat atttttcgtg attaatatgg
     5281 ggggattatg gatcagcaat atcttgagct gttacaagcc atgccgattt ttggtggtgt
     5341 tagccaacaa gccttgtcat ttttattaac acgctgccca gaaattgaga ttgcccaaaa
     5401 tgcctatttt tttaaagagg gtgagcaagc agattctttg tttattttgg agcgcggcac
     5461 ggcattagtg ttaaaagggc gagacatcaa attaagatat ttggtgcgtg gtgattgttt
     5521 tggtgaaatg tcattgattg atttaaaccc acgtagtgcc tcagtacgag cgattgagcc
     5581 atgtattgca ttggagctgt ctagacaaag tttatttgca ttgtatgagt atgatttaga
     5641 acaatttgtc attattcaaa tgaatttatc acgtgaggtg agccgacgtt tacgcgcctt
     5701 agatgagcgt ttaatgtctt tggatacgga attattaaaa ctgataaatg ttttataatt
     5761 tgttggacga tatgtatgta agttgatata gtcaaaaaac aatcaccatt aagatctaac
     5821 tatatcaaca tgaatacagc cattacccaa ttattagcca ttaaatacc
//
LOCUS       DAOQMB010000114         5822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6006_length_5822_cov_18.146003, whole genome shotgun sequence.
ACCESSION   DAOQMB010000114 DAOQMB010000000
VERSION     DAOQMB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6006_length_5822_cov_18.146003"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            572..2839
                     /locus_tag="PLY05_05395"
     CDS             572..2839
                     /locus_tag="PLY05_05395"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HNC02773.1"
                     /translation="MDEALRKAALDYHEFPHPGKIWVTPSKQLTNQRDLALAYSPGVA
                     APCLEIEKDPTTVSRYTARGNLVAVISNGTAVLGLGDIGPLASKPVMEGKGVLFKKFA
                     GVDVFDIEIDERDPDKLVEIIASLEPTFGGINLEDIKAPECFYIEKKLRERMKIPVFH
                     DDQHGTSIIVGSGLLNALKLVNKKIEDIKIVCSGAGAAAISCLDLLCAIGARRENIYV
                     ADSKGVLHFGRQMDESKAKYAQETDARTLGDIMKGADMFLGLSTAGLVTRDMVASMAD
                     KPIIFALANPTPEIMPEEIKAVRSDAIIATGRSDYPNQVNNALCFPYIFRGALDVGAT
                     TINEAMKIASVHAIARMAHVERDEVAYGEPNSFGAEYIIPKALDQRLIMEIAPAVAKA
                     AMDSGIATRPIKDFDAYRQRLSEFVYNSAFIMKPVFAQAKAAPNKRIVFCEGEDERVL
                     RAVQITVDEGLAHPLLVGRANMINSQIEKLGLRIRAGSDFTLIDQDNLPHYEKLWNYY
                     YDLMCRRGMGIELAMREVRRRSTLIGALLVQFGEADGMICGTFGHYHLHLDYIEQIIG
                     LNANAQNFYAMNVLMTEDRNLFIADTYVNPEPSSQQIAEMTLLAAEEIRRFGIVPKAA
                     LLSHSNFGTENTASAIKMRQAYALIRANAPELEVDGEMHGDAALNEEIRKATFPKSNL
                     TGNANLLVMPTLDAANIAFNLLKTASGSGVTIGPILLGAAKPVHVLTPSATVRRIVNM
                     VALTVAEILAKESQA"
     gene            3095..3736
                     /locus_tag="PLY05_05400"
     CDS             3095..3736
                     /locus_tag="PLY05_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02774.1"
                     /translation="MTKSILNQIISCAKTHRGEQRREALLKAARLIFLEKGFAGTSLD
                     DVIKITGGSKASVYKYFGNKDGLFAAMFAERCQVFLAHLDIPQGLSDNLEHTLTQFAQ
                     RVLESAIDVERIALIRALATEADRFPQLAEMAYNTGPKYVLDLLASLIKRHHDAGLLV
                     CPRPDIAAMQFMETIKAHAQWRTLLGLPAFANAINADDYIHIAVTNFLRAYQP"
     gene            3745..3945
                     /locus_tag="PLY05_05405"
     CDS             3745..3945
                     /locus_tag="PLY05_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02775.1"
                     /translation="MNKHQLSEDDFKVEGHCCAISIPAVRTATQPIDFDQQRLKDYLA
                     AHYPQHTSDTEKPSESPSLSDS"
     gene            complement(3931..4593)
                     /locus_tag="PLY05_05410"
     CDS             complement(3931..4593)
                     /locus_tag="PLY05_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02776.1"
                     /translation="MTETLSNDKKKRSFRGVSAEQRQAERQQKLLEAGLQCFGTHGFY
                     KVTVREICAEAKLTERYFYESFKNTESLLVAVYQRLIHDMQNVIIMTVLNAEDKQPKA
                     LIRLGLTTFLRYVSTDPRIARILFIDVPHVRFENKALIMDTMSTFDTMLQQFGRILFP
                     NFPNQGVNVELVTSGLNGANVHIVMRWVHSGFSYPFEEVLESCYTIFAAMAEYLEKLT
                     IT"
     gene            4685..>5822
                     /locus_tag="PLY05_05415"
     CDS             4685..>5822
                     /locus_tag="PLY05_05415"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC02777.1"
                     /translation="MNTISPDYQIIVIGTGFSGLGMGIALKKAGINDFAILEKADDVG
                     GTWRDNHYPGCACDVQSHLYSFSFEPNPNWSRMYAPQPEIKKYLQHCARKYGLLSHIQ
                     FGQQVKQAVYEEDYKLWRIETAAGKTLTARVVVGGMGGLSTPAYPNIKGLNKFKGVKF
                     HSQDWQHDYALEGKRVAVIGTGASAIQFVPQIVPKVAHLDLYQRTPPWIMPKPDRDIT
                     SAERTLFKLAPKSQEAIRNAIYLQLESRVLGFVLNPRVMKLPELQARRHIRQAIKDPV
                     LRKKVTPDYAFGCKRVLISNDYLPSLAQPNVDVITHGIKEVRANSIIGDDGVERPIDA
                     IIFGTGFKAQDPIPRGAIFGKGGVDLLDTWKEGAQAYLGSTMSGF"
BASE COUNT         1713 a         1184 c         1131 g         1794 t
ORIGIN      
        1 aatcctaaaa taatagcggc tgttgttttt tgataataac agttatctgc cacagcccct
       61 acagctaaaa aaattttcgt ccaagctttg ttgcttgcta atatcggcaa gacatagtat
      121 ttttatcaac aataccccgc tagacaatgc ttatagtaat aagcaatgat ctttggcttt
      181 aaaatcaagc tcatcacccc acgagagatt ttactggccg catattaagt gtctatgcca
      241 cttaattgcc cctcaccaag ggaaagttca tattttttgc aatgacattg ccacacgcaa
      301 tgcgtacagt gttatattca ctcactttat tttttcaagc ttagcatggc taagttttgg
      361 acttatgttg ttatcactga tttgctcgta tttaaactgt ttttatggtt actgagcgta
      421 gcttttatgc tctcacaacg tgctaatttg agtgaaacgc ctaagtcctc agtttatatt
      481 tttattgtat ttgacaccca ttgagcaacg actattttta tcgagtatca gtcgtttaat
      541 aaaagctctt tttgcacaag cgagacatac tatggacgag gcattacgta aagccgcctt
      601 ggattatcat gaatttcctc accctggtaa aatttgggtg actccaagca aacaattaac
      661 taatcaacgc gatttagcat tggcttattc accgggcgtg gctgcgccgt gtttagaaat
      721 cgaaaaagac ccaactactg tctctcgtta caccgcacgt ggcaacttgg ttgcggtcat
      781 tagtaatggt acagcggttt taggtttggg tgatattggc ccactcgcct ctaaacctgt
      841 gatggaaggt aaaggcgttt tatttaaaaa attcgcaggt gttgatgttt ttgacatcga
      901 aattgatgag cgcgaccctg acaaattggt tgaaattatt gcctctttag agccaacttt
      961 tggtggcatt aacttagaag atattaaagc acctgaatgt ttttatatcg aaaagaaact
     1021 ccgcgaacgc atgaaaatcc ctgtgtttca tgacgatcaa catggaacat cgattattgt
     1081 tggctctggt ttgttaaatg ccttaaaact ggttaacaaa aaaatcgaag atattaaaat
     1141 tgtttgttct ggcgcaggtg cggcagctat ttcatgttta gacttattgt gtgcgattgg
     1201 cgcacgccgt gaaaacattt atgtcgctga ctctaaaggt gttttacact ttggccgtca
     1261 aatggacgaa agtaaagcca aatatgccca agaaactgat gctcgcacct taggcgacat
     1321 tatgaaaggt gcagatatgt tcttaggctt gtctacagca ggcttagtca cgcgtgacat
     1381 ggttgctagc atggccgata agccaattat ttttgctttg gctaacccta ctcccgaaat
     1441 tatgcccgaa gaaattaaag cggtgcgttc ggatgcgatt attgccacag gccgttctga
     1501 ctatcccaac caagtcaata acgccttatg tttcccttat attttccgtg gtgcattaga
     1561 tgtaggcgca accaccatta acgaagccat gaaaattgct agcgtacacg ctattgcacg
     1621 tatggcacac gttgaacgtg acgaagtagc ttatggcgaa ccaaacagct ttggcgcgga
     1681 atacattatt cctaaagcct tagaccaacg tttgattatg gaaattgcac ctgcggttgc
     1741 taaagcagca atggattctg gcattgccac acgcccgatc aaagattttg atgcttatcg
     1801 ccaacgtttg tctgagtttg tttataactc tgcatttatt atgaagcccg tttttgccca
     1861 agcaaaagca gcaccgaaca agcgtatcgt tttctgtgaa ggtgaagatg agcgtgtatt
     1921 acgtgccgta caaattaccg ttgatgaagg tttagcgcat ccattattag tgggtcgtgc
     1981 caacatgatt aatagccaaa ttgaaaaatt aggcttgcgt attcgtgctg gtagtgactt
     2041 taccttaatc gaccaagaca acttacctca ctacgaaaaa ctctggaact actactatga
     2101 cttaatgtgt cgtcgtggga tgggcattga gttagccatg cgtgaagtac gtcgtcgtag
     2161 taccttaatt ggcgcattgt tagttcaatt tggcgaagct gatggcatga tctgcggtac
     2221 atttggtcac taccatttgc acttagatta tattgagcaa attattggtt taaatgccaa
     2281 tgctcaaaac ttctatgcaa tgaatgtatt aatgactgaa gatcgcaatt tatttattgc
     2341 cgatacttac gttaatccag aacccagcag ccaacaaatt gctgaaatga cgttattggc
     2401 tgccgaagaa attcgtcgtt ttggtattgt gcctaaagct gctttattgt ctcactctaa
     2461 ctttggtaca gaaaatacgg ccagtgccat caaaatgcgc caagcttatg ccttaattcg
     2521 tgccaatgca ccagagttag aagttgatgg tgaaatgcat ggtgatgcag ccttaaacga
     2581 agaaattcgt aaagccacct tccccaaatc taatttaaca ggtaatgcta acttgttagt
     2641 tatgccaacc ttagatgcag ccaatattgc ctttaattta ttaaagactg cgtctggtag
     2701 tggtgtcact attggcccaa tcctcttagg tgcggcaaaa cctgttcacg tgttaacacc
     2761 ttctgccaca gtacgtcgta ttgtgaatat ggttgcgtta accgttgctg aaattcttgc
     2821 caaagaatct caagcttaaa cctcactaaa gagaggatat caacgatatc ctctcttgtc
     2881 tttttttgcc ttcctcgtta actttctgct attgttattc attagctctt taactacgct
     2941 taatatcaat aaatacttag ctatttaaaa cttataacaa ctattgaatt atttaagcaa
     3001 tcttgataat gtactgacaa gtacaatttc atttatttaa aaatatctaa ttaaatcaat
     3061 atattaaaaa tatcagtact agatactaag caaaatgact aaatccatat taaatcaaat
     3121 cattagttgt gcaaaaacac atcgtggcga acagcgacgt gaagcattat taaaggctgc
     3181 gcgtcttata tttttagaaa aaggatttgc tggaacaagc ttggatgatg ttattaaaat
     3241 aactggtggt tcaaaagcca gtgtttataa atattttggc aataaagatg gtttatttgc
     3301 tgcaatgttt gctgagcgtt gtcaggtttt tttagcccat ttagatattc cacaaggatt
     3361 aagtgataat ttagagcata cactcactca atttgcacaa cgtgttttag aatcagctat
     3421 tgatgtggaa cgaattgcgc taattcgagc attggccaca gaggctgatc gttttcctca
     3481 attagctgaa atggcttata acacagggcc aaaatatgtt ttagatttat tagccagttt
     3541 gatcaaacgg catcatgatg ctggtttgct tgtctgtcct cgcccagata tagctgccat
     3601 gcaatttatg gaaaccatca aagctcacgc tcaatggcgc accctactcg gactgcctgc
     3661 ttttgctaac gctatcaatg ccgatgatta cattcacatt gctgtcacta attttttacg
     3721 tgcatatcaa ccctagaggc cgacatgaat aaacatcaat tatccgaaga cgattttaaa
     3781 gtcgaaggcc attgctgtgc gattagcatt cctgctgttc gtactgccac acagccgatt
     3841 gattttgatc aacaaaggct aaaagattat ttagcagcac attatcccca acatacctct
     3901 gacacagaaa aaccatcaga aagtccatca ttaagtgata gttaattttt ctaaatactc
     3961 agccattgcc gcaaaaatgg tgtaacaact ttctaacact tcctcaaacg gataactaaa
     4021 accactatgc acccaacgca taacaatatg gacgtttgca ccatttaaac ccgatgtcac
     4081 taattccaca tttacgccct gattaggaaa attaggaaac agaattcggc caaattgctg
     4141 caacatggta tcaaaggtac tcatggtatc cataatcaaa gctttatttt caaagcgcac
     4201 atgtggcaca tcaataaata agatacgggc aatacgtgga tcggttgaga cataacgcaa
     4261 aaacgtagtt aaacctaatc taattaacgc tttaggttgc ttatcttcgg catttaacac
     4321 cgtcatgata atcacgtttt gcatatcgtg aattaaacgc tgataaaccg ccaccaataa
     4381 actttcggta tttttaaacg attcataaaa atagcgttcg gttaatttgg cttcagcaca
     4441 aatttcgcgc acagtcactt tataaaagcc atgtgtacca aaacattgca atcctgcttc
     4501 taacaatttt tgctgacgtt ccgcttgtcg ttgttcggca ctcaccccac gaaaagaccg
     4561 ctttttcttg tcattggata atgtttcagt catttttgta aaaaacctac gtttagatgt
     4621 tgacatcaat cgttgtcaat tataaagttg acaacactta ttgtccagtt tggtaacata
     4681 atttatgaac acgattagtc cagattatca aatcattgtt attggcacag ggttttctgg
     4741 cctaggtatg ggtattgcct taaaaaaagc aggcattaat gattttgcca ttcttgaaaa
     4801 agctgatgat gttggtggta catggcgtga taaccactat cctggttgtg cgtgtgatgt
     4861 gcaatctcac ttatattctt tctcgtttga acctaatcca aactggtcaa gaatgtatgc
     4921 tcctcaaccc gaaattaaaa aatatttgca acattgtgca cgcaaatatg gtttgttaag
     4981 tcatattcaa tttggtcaac aagttaaaca agccgtttat gaagaagact ataaattatg
     5041 gcgcatcgaa acagccgcag gtaaaacctt aaccgcccgt gttgtggtgg gtggcatggg
     5101 cggtttgagc acccctgctt atccaaacat taaaggttta aacaaattta aaggcgttaa
     5161 attccactct caagattggc agcatgatta tgcgcttgaa ggcaaacgtg tcgcggtgat
     5221 tggtacaggt gcaagtgcta ttcaatttgt gccacaaatt gtgcctaaag tcgcgcattt
     5281 agatttatat caacgcacgc caccttggat tatgccaaaa cctgatcgtg acattaccag
     5341 tgcagaacgt accttgttta aacttgcgcc taagagccaa gaagccattc gtaatgccat
     5401 ttatttgcaa ttagaaagcc gtgttttagg ttttgtactt aatccacgtg tgatgaaatt
     5461 gcctgagtta caagctcgtc gccatattcg tcaagcgatt aaagaccccg tcttacgcaa
     5521 aaaagtcacg cctgattatg cctttggttg caaacgcgtc ttaatttcta atgactattt
     5581 accctcatta gcacaaccca acgttgatgt aattactcac ggcattaaag aagtacgtgc
     5641 caatagcatt attggtgatg atggtgttga acgtcccatt gatgcgatta tttttggcac
     5701 aggctttaaa gcccaagatc caattcctcg tggtgctatt tttggtaaag gtggcgtcga
     5761 tttattggat acatggaaag aaggcgcaca agcttactta ggtagcacca tgagtggttt
     5821 cc
//
LOCUS       DAOQMB010000115         5770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6088_length_5770_cov_17.614348, whole genome shotgun sequence.
ACCESSION   DAOQMB010000115 DAOQMB010000000
VERSION     DAOQMB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5770
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6088_length_5770_cov_17.614348"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /gene="pyrH"
                     /locus_tag="PLY05_05420"
     CDS             complement(<1..723)
                     /gene="pyrH"
                     /locus_tag="PLY05_05420"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNC02778.1"
                     /translation="MVERRPTYQRILLKLSGEALAGDADMGIDPKVLNRMALEVGQLV
                     GIGVQVGIVLGGGNLFRGAALQAAGLDRVAGDHMGMLATVMNGLAMRDALERNNIKTR
                     IMSAIPMSGVVDHYDRRAATRALQSGDVCVFVAGTGNPFFTTDTAACLRGIEINADVV
                     LKATKVDGVYDADPFKNPDAVKYDYLTFDEVLDKKLGVMDLTAICLCRDQSMPLRVFD
                     MNKQGALLNLVCGQSEGTLIGNK"
     gene            complement(826..3606)
                     /locus_tag="PLY05_05425"
     CDS             complement(826..3606)
                     /locus_tag="PLY05_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNC02779.1"
                     /translation="MWSTILAALELTPEEQAWIANHPVIRVGVDPAWPPYEFMDEQGN
                     HQGMSADYLALLSQKIGVKFQIAPAQPWSDTQQQLEQKQLDFTPSIVSTPKRDQFLNF
                     SQPYISFPVVILTRSDEAFIGQLEDLSGKRVGVEADYYADEILQQNYPTIQRVRYPYL
                     RDLLSALALGQVDYVLTNYASASYAVQQLHMTGLKLAAVTQYQSPLSMGVRKDWPEFV
                     SILNKALNELTPEQHQRIRQHWLGIKKENIYIRDIWRLHPDIILLLLMILMFVVLLAI
                     VFYFRHRLMTRKLLAEKAQQELAESEKRFRLLIENAPIAFAIFKGKVGIIKMLNRTFI
                     NMFGYQPSELHDVEDWWRVAYPNPSYREQIQKIWYERLELARKSHQVLEPMEATVRCR
                     DGSERYIRFHAILIGSLSLVAFIDLTDQKKYQTALLTAKENAEQATQAKSLFLANMSH
                     EIRTPMNGILGLTALLEKTELNERQHDYVTKIYSSGEFLLGLLNDILDLSKVEAGKLE
                     LEKQPFTIAQLLEPLRALALSATQHKPVEVLFYVSPDVPKVLLGDLLRLGQILTNLLG
                     NAIKFSAKGEIEIGVATITNKNHQQRLHLWVRDTGIGMTTEQAAKIFEAFSQADASTT
                     RKYGGTGLGLTICKRLIELMNGEIKVQSKVNQGSRFDVFIDLIEAPQDSSSLESINIP
                     KLNLLLIDNNHHSALAIRSIAERLQWEVDIFERLDESLTIETSHYDFVLLDADFADEH
                     SVQYLPKALPKILLQTPLQESDPLLGAMIDFKHCFTKPITEEVLLGLVPSAIPKAKFI
                     EEKPFLARRILLVEDNTINQLVGVRLLESLGAEVDVAENGYVALSLLQQVGNFYDVVL
                     MDLQMPVMDGLETTRQLRLIEQFTDLPVIAMTANVMATDREACFDAGMNDFMSKPIRI
                     QELREKIYQWLK"
     gene            complement(3767..4657)
                     /gene="tsf"
                     /locus_tag="PLY05_05430"
     CDS             complement(3767..4657)
                     /gene="tsf"
                     /locus_tag="PLY05_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNC02780.1"
                     /translation="MAEITASLVKELRERTGLGMMECKKALAEVGGDIEVAIDNLRKS
                     GATKAAKKAGNVAAEGAILVANSADGKTSLLLEVNSQTDFVAKDANFAQFANKVAQIA
                     LANGATDVATISGLSYDEGVTVEEARVALVQKIGENIQIRRATLVQGDIVATYIHGLK
                     IGVAVALTGGDAELGRDVAMHIAASNPMVINPADVPADVLAREKEIYAAKARESGKPE
                     NIVEKMIEGSLKKYLAEVSLTEQAFVKDPDVTVGALLKKAGATLNSFTRLEVGEGIEK
                     KEVDFAAEVAAQQAAAAGNK"
     gene            complement(4772..5524)
                     /gene="rpsB"
                     /locus_tag="PLY05_05435"
     CDS             complement(4772..5524)
                     /gene="rpsB"
                     /locus_tag="PLY05_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNC02781.1"
                     /translation="MAQVSMRDLLQAGAHFGHQTRFWNPKMGQYIFGARNKIHIINLE
                     HTVVALNDALTYANGLAAKRNKVLFVGTKRAASGIIRAEAQRCGMPYVDHRWLGGMLT
                     NWKTIRQSIKRFKELEQQAQDGTFAKLTKREALERTREMEKLEKSFGGIKDMGGLPDA
                     LFIIDVDHEAIAIQEARKLGIKVIGVVDTNSNPDGVDIIIPGNDDAIRAIQLYAGSMA
                     DAILEGKEYAATQAGGREPEATEEAAAPEETA"
BASE COUNT         1763 a         1268 c          984 g         1755 t
ORIGIN      
        1 tttattgcca attaatgtgc cttcactttg accacaaaca aggtttaata atgcaccttg
       61 tttattcatg tcaaacacac gcaatggcat actttggtca cgacataaac aaatagcggt
      121 taagtccatg acacctaatt ttttatccaa tacttcatca aaggttaaat aatcatattt
      181 gacagcatca ggatttttga aaggatcggc atcatatacg ccatccactt tagtggcttt
      241 taacaccaca tcggcattaa tttcgatacc acgtaaacaa gcagcggtat ctgtagtaaa
      301 gaatggatta cctgtgcctg caacaaacac acacacatca ccgctttgca aagcgcgagt
      361 tgcggcacga cggtcataat ggtcaaccac accactcatt ggaattgcag acataatacg
      421 ggttttaatg ttattgcgct ctaaagcatc acgcatggct aaaccattca taacagtggc
      481 caacataccc atatggtcac ctgcaacacg atctaagcca gctgcttgca aagctgcacc
      541 acggaacaaa ttaccaccgc ctaaaacgat accaacttgt accccaatac ccactaattg
      601 acccacttct aaagccatac gatttaagac tttaggatca atacccatat ctgcatcacc
      661 tgctagggct tcacctgaga gttttaatag aatacgttga taagtaggac gacgctcaac
      721 catgtgtttc tcctttggtg atttggcact aaatagccgc gcatcttaac agaaagcagc
      781 ctttggcaaa atagagcgat gactaaggat ttatcgttgt ttgaattatt ttaaccattg
      841 ataaattttt tcgcgtaatt cttgaatcct aatcggttta ctcataaagt cattcatgcc
      901 tgcatcaaaa caggcttctc tgtctgttgc catgacattc gccgtcattg caattacagg
      961 taagtcggtg aattgctcta ttaagcgtaa ttgccttgta gtttctaaac catccataac
     1021 aggcatttgt aaatccatta ataccacatc ataaaaatta cccacttgtt gtaatagact
     1081 taaagcaaca taaccatttt cggcaacatc aacctctgcc cccaaagatt ccaacaaacg
     1141 cacaccaact aattgattaa tggtgttgtc ttctaccaat aaaatacgtc gggctaaaaa
     1201 aggcttttct tctataaact ttgctttagg gatggcacta ggcactaaac ctaataacac
     1261 ttcttcggta ataggtttag taaaacaatg tttaaaatca atcattgccc ctaacagagg
     1321 atcagattct tgcaaaggcg tttgcaataa aatcttaggc aacgctttgg gtaaatattg
     1381 tacgctatgc tcatcagcaa aatcggcatc taataagaca aaatcataat gacttgtctc
     1441 aatggttaat gattcatcta gacgttcaaa aatatcgact tcccattgta aacgttcggc
     1501 aatagaacgg atcgccaatg cggaatgatg attattatct attaataata aattgagttt
     1561 gggtatattg attgattcta acgaagaact atcttgtggg gcttcaatca agtcaataaa
     1621 tacatcaaaa cgactacctt gatttacctt gctttgcact ttgatttcgc cattcattag
     1681 ctcaatcaat cgtttacaaa tggttaaccc caatcctgtt ccaccatatt tacgagttgt
     1741 agatgcatct gcttggctaa atgcttcaaa aattttggct gcttgctcag tcgtcatgcc
     1801 tatgccagta tcacgcaccc ataaatgcaa acgttgttgg tgatttttat tggtaatcgt
     1861 agcaacacct atttctattt cacccttcgc gctaaactta attgcatttc ctaaaaggtt
     1921 agtcaatatc tgaccaagac gtaataagtc gcctaataat acttttggaa catctggcga
     1981 aacataaaat aatacttcta caggcttatg ctgtgtggca cttaatgcta aagctcgcaa
     2041 tggctctaat aattgcgcaa tagtaaatgg ttgtttttct agctctaatt ttcctgcttc
     2101 aaccttagaa agatccaaaa tatcatttaa taagcccagc aaaaactcgc ctgaactata
     2161 aattttagtc acataatcat gttgtcgttc attaagctca gttttttcta aaagcgcagt
     2221 tagccccaaa ataccattca ttggtgtgcg aatttcgtga ctcatattag ccaaaaataa
     2281 acttttggct tgtgtcgctt gctcggcatt ctcttttgct gtcaataaag cagtttgata
     2341 ttttttttgg tcggtcaaat caataaaagc taccaaactt aaagaaccaa tcaaaatagc
     2401 atgaaaacgg atgtaacgct cactgccatc acgacaacga accgttgctt ccattggttc
     2461 taagacttga tgacttttac gggctaactc taaacgctca taccatattt tttgaatttg
     2521 ctctctataa ctaggattag gataggctac tcgccaccaa tcctccacat catgtaattc
     2581 actaggttga taaccaaaca tattaataaa tgttcgattt aacattttga taatcccgac
     2641 tttaccttta aaaatcgcaa aagcaatcgg ggcattttca attaataaac gaaaacgttt
     2701 ttcgctttct gctaattctt gttgtgcttt ttcggctaat aatttacgcg tcattaaacg
     2761 atgacgaaag taaaaaacaa tcgctaacaa caccacaaac atcaggatca ttaacaataa
     2821 taagataata tctggatgca aacgccaaat atctctaata taaatatttt cttttttaat
     2881 ccccaaccaa tgttgtctaa tgcgttgatg ttgctctggt gttaactcat ttaaggcttt
     2941 atttagaata gacacaaatt caggccaatc tttacgcact cccatcgata atggtgattg
     3001 atattgtgtc actgctgcca gctttaaacc tgtcatatgc aattgctgta cggcatagga
     3061 tgctgaggca tagttagtca atacataatc aacctgacct agagccaatg ctgataacaa
     3121 atctcgcaaa tagggataac gcactctttg tattgtggga taattttgct gcaagatttc
     3181 atctgcataa taatctgctt ctacaccaac acgttttcct gataaatctt ctaactgccc
     3241 aataaaagct tcatcagatc gggttaaaat gacaacagga aaagaaatat aaggctggga
     3301 aaagtttaaa aactgatcac gtttgggggt agaaacaata gaaggcgtaa aatctaattg
     3361 tttttgctct aattgttgtt gggtgtctga ccatggttga gctggtgcaa tttgaaattt
     3421 gacacctatt ttttgcgaca ataacgccaa gtaatcggca ctcattccct gatgattacc
     3481 ttgttcatcc ataaactcat aaggtggcca tgcaggatca acacctactc gaatcacagg
     3541 atgatttgct atccaagctt gttcttcggg ggttaactct aaagctgcca atatggtcga
     3601 ccacatgacc aatatcaaca tacttacatg aaccaccaaa gtcctcagcc ctatgcgcat
     3661 tattattatc atcctaaagc aaagattctc aagaatttac catatactaa gggctaaaac
     3721 aaaaataccc gcttaaagcg ggtattttta tgagtaacaa taaaacttat ttgttaccag
     3781 cagcagcggc ttgttgagca gcaacttcag cggcgaagtc aacttctttc ttctcaatgc
     3841 cttcacccac ttctaaacgt gtgaagctgt tgagtgttgc acctgctttc tttaataaag
     3901 caccaaccgt tacgtctgga tctttaacaa aagcttgttc tgttaagcta acttctgcta
     3961 agtacttttt caagctacct tcaatcatct tctcaacgat gttttctggt ttgccagatt
     4021 cgcgagcttt agcagcataa atttcttttt cacgcgctaa aacgtcagca ggaacatcgg
     4081 ctgggttgat aaccattggg ttgcttgcag caatgtgcat agccacatca cgacctaatt
     4141 cagcatcacc acctgttaaa gcaacagcaa cgccaatttt taaaccgtga atataagttg
     4201 caacgatgtc accttgtact aaagtagcac gacggatttg gatgttttca ccgatttttt
     4261 gaaccaaagc aacacgagct tcttcaacgg tcacgccttc gtcgtagctt aaaccagaaa
     4321 tagtagcaac atcagttgca ccgttagcta aagcaatttg agcaacttta ttagcaaatt
     4381 gtgcaaagtt agcatcttta gcaacaaagt cagtttgact gttaacttct aacaataaag
     4441 aagttttgcc atctgcactg ttagcaacca aaattgcacc ttctgcagca acgttacctg
     4501 cttttttagc agctttagtt gcaccagatt tgcgtaagtt gtcgatagcg acttcgatgt
     4561 caccacctac ttcggctaat gcttttttgc attccatcat gcctaaacca gtacgctcac
     4621 gtaattcttt aacgaggcta gcggtaattt ctgccatgtt gatatcctct taattgctaa
     4681 tcaaacttaa aacaagataa gtttttgttg aaaatcatgt aattgataaa aagaaaggga
     4741 gactaagctc cccttctctt tgcttaaaaa attaagctgt ttcttctgga gcagcagctt
     4801 cttcagttgc ttctggttca cgaccaccag cttgtgttgc agcgtattct ttgccttcta
     4861 aaatagcatc agccatagaa ccagcatata attgaatagc gcggatcgcg tcgtcgttac
     4921 caggaataat gatgtcaaca ccatctgggt tagagttagt atcaaccaca ccgattactt
     4981 tgatgcccaa tttacgagct tcttgaatag caatcgcttc atggtcaacg tcgatgataa
     5041 ataaagcatc tggcaaaccg cccatatctt taataccacc aaagctcttt tctaactttt
     5101 ccatttcgcg agtacgctct aaagcttcac gcttagtcaa cttagcaaaa gtaccgtctt
     5161 gagcttgttg ttctaactct ttgaaacgct tgatagattg gcgaattgtt ttccagttgg
     5221 ttaacatacc acctaaccaa cggtgatcaa cgtatggcat accacaacgt tgtgcttcag
     5281 cgcggatgat gccagaagca gcacgcttag taccaacaaa caatactttg ttgcgcttag
     5341 cggctaaacc gttggcataa gttaaagcat catttaaagc aacaacagtg tgctctaagt
     5401 tgatgatatg aattttgtta cgtgcaccga aaatgtactg acccattttt gggttccaga
     5461 aacgggtttg gtgaccgaaa tgtgcacctg cttgtaacag gtcacgcata gaaacttgtg
     5521 ccatgattaa ctccaattag ggttaggcct ccatatatcc caattaccga ctgtcgtttg
     5581 cacgcccagc acccaataat tgtgtcgata tatgtgcgtt atgtgtaaaa ttaatttaga
     5641 cttacacgcc agaaagttac gcgcttatct ctttgttcga ttttatccaa acaaggcgcg
     5701 ctttatacca tactcgctgt ttagcctcaa tcgtgattta aaaatttatg actgtttctc
     5761 ttaaaactgc
//
LOCUS       DAOQMB010000116         5748 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6123_length_5748_cov_5.663271, whole genome shotgun sequence.
ACCESSION   DAOQMB010000116 DAOQMB010000000
VERSION     DAOQMB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5748
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6123_length_5748_cov_5.663271"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..778)
                     /locus_tag="PLY05_05440"
     CDS             complement(68..778)
                     /locus_tag="PLY05_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02646 family protein"
                     /protein_id="HNC02782.1"
                     /translation="MRSITKIGNGGYQLNRSHQTPPTTSDQATSRWSSFGYKEEVTQS
                     LLAEQYGLCAYSEVRPDQLGLGVHIEHIEPKSANPARTFDYSNLVLSALSSDDLKTID
                     KTAVFGGHAKLSQYDALLFISCLQADCGRYFVYLSNGNVEPAYTLEPEQKSKAQYTID
                     LLNLNSPYLVNQRKNWIDELDELIDEHIQKNMSLDHLAAIDLLPFNNKLSPFFTATRQ
                     RFGQIANQLLAANASELS"
     gene            complement(775..2157)
                     /locus_tag="PLY05_05445"
     CDS             complement(775..2157)
                     /locus_tag="PLY05_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02783.1"
                     /translation="MKIKNITIKNFRCFEQLEVTFHPQMTVLIAVNGGGKTSILDAVR
                     IAVWPFVKGFDLGSQTGKSATIQTGDVRLEKHINGNMEPKPKTEVNTTGVWDSTSQVK
                     TWLQWRDSIKKGTNSKGDTSTVELTNQYAKSMQKKVFEGKEQIDLPLIAYLGTGRLWY
                     QSRYSSEAADVVLSKTEHSRTSGYLNCLSQGSSFKHFVDWYGWVYRSYREEQIKALEK
                     GYSWGEPSSHFLQTIKAIQFAVNCLIEKQTGWKDIAYSSSHEQQIVLTHPQHGTLPLE
                     RLSDGLRNMVAMVADLAYRCVKLNPHLGAEATIKTHGIVMIDEVDMFLHPSWQQSVLG
                     SLQEAFPKIQFIVTTHSPQVLSTVPKECIRVLGTNAEGENIAAMPLADSYGEPSNDVL
                     QAIMQVDPQPPVPEKRLLDELTSLVDQGEYQSSRATELLANLKQKLSNQHPQILKIER
                     SIRRQEAFKA"
     gene            complement(2154..5693)
                     /locus_tag="PLY05_05450"
     CDS             complement(2154..5693)
                     /locus_tag="PLY05_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC02784.1"
                     /translation="MLKLEDVKKDAQIRGIQAEEIVRIVQVEPVGDAAITVYYKDSQG
                     KLGEQMLFRSDESRLELATAGRPWAFDAPGAEFKLGLEAYRITQAALFDPMMAVHTSN
                     VEPLPHQISAVYEYMLPKQPLRFVLADDPGAGKTIMAGLLIRELLMRADAKRILIVSP
                     GSLTEQWQDELLEKFGVTFDIFSREKQEQCASGNYFDESDQLICRLDQLSRNEDFQEK
                     LKNTDWDLIIVDEAHKLSANYFGNKVNKTKRFQLGELLGSITRHYLLMTATPHNGKEE
                     DFQIWLSLLDGDRFYGKFREGVHKVDISDMMRRMVKEELLKFDGTPLFPERRAYTANY
                     ELSPMEASLYEQVTTYVREEMNRADNLDGQKKNNVGFALTMLQRRLASSPEAIYQSLK
                     RRRKRLEDRLAETKLMARGQSVKSNHIAETIGEYSVKKKIDLPENLDQLDEDLSAAEY
                     ELYVEQVVDQASAAETIPELEAEILSLKDLEYQALSVVQSGNDKKWEELSCLLQDKPE
                     MFTASGSRRKLIIFTEHKDTLNYLVVRISDMLGNTNSVRTIYGGTNRDERRKIQEEFR
                     NDPEVIVLIATDAAGEGVNLQNANLMVNYDLPWNPNRLEQRFGRIHRIGQTEVCHLWN
                     IVANETREGEVFQKLFDKIEIEKEALGGKVFDVLGEAFDNVSLRDLLVQAIRYGEDPK
                     VKAKMNEVIEGALDAEHLKEIIKRNALVDQHMGLEDLYAVKEEMEKAEARKLQPYFIR
                     AFFTEAFQTLGGEFRSRESGRYEVRHVPAVIRERDRIIGESRTPVLKKYERICFEKQH
                     VRQTGKPMADMIHPMHPLMHATTDLILQAYRAKLKQGAMLVDPNDDSINPKVLFMVDH
                     TVRESNQSDEVASRRLQFVEINEQGIAINAGWAPHLDLQPIEDYDRKLVGDILQSHWL
                     NHNLEALALNYASQKLVPEHYAEVKGRRERQANKTMAAVNERLVKEVNYWSDRYIKLQ
                     DDVSAGKQPRMQPEMARRRVDELTARLEQRKAELIALKNVVSSTPVVIGGALVIPQGL
                     LSMRKGESQFGVDAIARAHVERVAINAVIQAEQAFGYKVTDVGAEKCGWDITSRPPAN
                     VDGSIKPDRHIEVKGRAKGQSTITVSRNEIIYGLNQSDKYILAIVIVDTDLHEGPFYI
                     KNPFTIEPDFGVASVNYDLGDLLSKAVKPEQTL"
BASE COUNT         1528 a         1283 c         1142 g         1795 t
ORIGIN      
        1 cttcgttaat ggcatctaaa ggcaatgagg tttcgatgag ttttttgggg gatttgattt
       61 gagtcattta tgataattcc gaggcattgg ctgcaagtaa ttgattggca atttggccaa
      121 agcgttggcg agtcgcggta aaaaacggac tgagtttgtt gttaaaaggc aataagtcta
      181 tggcggccaa atggtctaag gacatatttt tttgaatatg ctcatcaatt aattcatcaa
      241 gttcatcaat ccaatttttg cgctgattga ctaaataagg gctgtttaaa ttgagcaaat
      301 caatggtgta ttgggctttg cttttttgtt caggctcaag tgtataagca ggctctacgt
      361 tgccattaga taaatacaca aagtaacgcc cacaatcggc ctgcaagcaa ctgataaaca
      421 acaacgcgtc gtattggcta agttttgcat ggccgccaaa aacagctgtt ttatcaatgg
      481 tttttaagtc atcactcgac agcgcactaa gtaccaagtt gctatagtca aaggtacgtg
      541 ctgggttagc actcttgggt tcgatatgct caatgtgtac acctaaacct aattgatctg
      601 ggcgaacctc tgagtaagca cataggccat attgttcggc taataaggat tgagtaactt
      661 cttctttata accaaaacta ctccaacggc tagtggcttg gtcgcttgtt gtgggtggag
      721 tttgatgaga cctatttaat tgataaccac cgttacctat ttttgtaatt gacctcatgc
      781 tttaaacgcc tcttgacgac gaatactgcg ttctattttt aaaatctgtg ggtgttgatt
      841 acttagtttt tgtttaagat ttgccaataa ctctgttgca cgagatgatt gatactcacc
      901 ttgatcaact aatgaagtaa gttcatcgag taagcgtttt tcggggactg gtggctgagg
      961 gtcaacctgc ataatggctt gtagtacatc gttactgggt tcaccatagg agtccgccaa
     1021 tggcatggca gcaatatttt caccttcagc attggtgcca agcacacgaa tacactcttt
     1081 aggcactgtg gaaagcactt gcggactgtg cgtagtaacg ataaattgaa ttttagggaa
     1141 ggcttcttgc aaagaaccaa gcaccgattg ttgccatgac ggatgcaaaa acatatccac
     1201 ttcatcaatc attacaattc catgtgtttt tatggtcgcc tctgcaccca aatgtggatt
     1261 tagcttgaca cacctgtaag ctaaatcagc caccattgcg accatgttgc gcaaaccatc
     1321 actcaaccgc tctaagggta aagtaccatg ttgcggatgt gttaaaacga tttgttgctc
     1381 atgacttgaa ctataagcaa tatctttcca acctgtttgc ttttcgatta aacaattgac
     1441 tgcaaattgg attgctttaa ttgtttgcaa aaaatgactg cttggctcac cccaactata
     1501 acctttttcc aatgctttaa tttgctcttc tcgataacta cgataaaccc aaccatacca
     1561 atcgacaaaa tgcttaaaac tagaaccttg agataaacag tttaaatagc cagaagtgcg
     1621 tgaatgttct gtttttgata acacaacgtc tgccgcctca gatgaatacc gactttgata
     1681 ccacaaacga cctgtaccta aataggctat taagggcaaa tcaatctgct ctttaccctc
     1741 aaaaaccttt ttttgcatag atttcgcata ttggtttgtt aattcgactg ttgatgtatc
     1801 acccttactg tttgttcctt ttttaatact gtctcgccac tgcaaccatg tttttacttg
     1861 gctagtgcta tcccaaacac ccgttgtatt aacctcggtc tttggttttg gttccatgtt
     1921 gccattgata tgtttttcta atcttacatc gccagtctga atcgttgccg actttcctgt
     1981 ttgactgcct aaatcaaacc ctttcacaaa cggccacaca gcaatacgaa ctgcatccaa
     2041 aatagaggtt ttacctcctc cattaacagc aattaatact gtcatttggg gatggaatgt
     2101 gacttctaac tgttcaaaac agcgaaagtt tttgatagta atatttttga ttttcataat
     2161 gtttgttctg gtttaacggc ttttgacagt aaatcaccta aatcgtaatt tacacttgcc
     2221 acgccaaagt ctggctctat tgtgaatgga tttttgatat aaaatgggcc ttcgtgtaaa
     2281 tcagtatcca caataacaat ggcaagaata tacttatccg attgatttaa tccgtaaata
     2341 atttcgttac ggctcacggt aatggtgctt tgacctttgg cgcgtccttt tacttctata
     2401 tgacggtcag gcttaataga gccatcgaca tttgcaggtg gacgcgaggt aatatcccag
     2461 ccgcactttt cggctcctac atcggtcact ttatagccaa aggcttgttc ggcttgtatt
     2521 accgcattga tggcaactcg ctctacatga gcgcgagcga tggcatctac accaaattgg
     2581 ctttcacctt tgcgcattga caacaatcct tgcggaatca ccaatgcacc accaataaca
     2641 accggagtgc ttgatactac gtttttaagc gcaatcaact cggctttacg ttgttctaat
     2701 cgagcggtaa gttcatccac acggcgacgt gccatttcgg gttgcatacg cggttgcttg
     2761 cccgcactca cgtcgtcttg tagcttaata tatcggtctg accaataatt gacttcttta
     2821 accaatcgct cattaacagc agccatcgtt ttattagcct gtcgttcacg acggcctttg
     2881 acttcggcat aatgctcggg taccaacttc tgtgaagcgt agttaagtgc taatgcttcg
     2941 agattatgat taagccaatg tgattgtaaa atatcaccca ctaattttct gtcataatcc
     3001 tctataggtt gtaaatctaa gtgtggtgcc caaccagcgt taatagcaat gccttgctcg
     3061 tttatctcaa caaactgtaa tcgacgagat gccacttcat cgctttggtt tgactcccgc
     3121 actgtatggt caaccataaa caatacttta ggattaatac tgtcgtcatt cggatcaacc
     3181 aacatagcac cttgtttgag tttggctcga tatgcctgta gtattaaatc ggtcgtggca
     3241 tgcattagtg ggtgcatggg gtgaatcatg tctgccatgg gcttgcctgt ttggcgtaca
     3301 tgttgctttt caaaacaaat acgttcatat tttttaagta ctggcgtgcg actttcgcca
     3361 ataattcgat ctcgttcacg aatcacagca ggtacatggc gcacttcgta gcggccagac
     3421 tctcgtgacc taaactcacc tcctagtgtt tgaaaagctt ccgtaaaaaa ggctctaata
     3481 aaatagggtt gtagtttacg tgcttccgcc ttttccattt cttcttttac ggcataaagg
     3541 tcttctagtc ccatgtgctg atcgaccaaa gcattacgct taataatttc tttgaggtgc
     3601 tcggcatcta atgccccttc aatcacctca ttcatttttg ctttaacttt aggatcttcg
     3661 ccatagcgaa tcgcttgaac caataaatct cgtagtgaaa cattatcaaa agcctcaccc
     3721 aacacgtcaa atactttgcc gccaagggct tctttttcta tttcaatttt atcgaacagt
     3781 ttttgaaata cttcgccttc gcgagtttcg ttagcaacga tgttccataa atgacagact
     3841 tcggtttgac caattcggtg gatacgtcca aaacgttgct ctaaacggtt tggattccaa
     3901 ggcaagtcgt agttgaccat taaattggca ttttgcaggt tgacaccctc gcctgcggca
     3961 tcggtcgcaa ttaacacaat aacttctggg tcattgcgaa attcttcttg aatttttcgg
     4021 cgttcgtctc ggttagtacc accataaatg gtacgaacag aattagtatt gccaagcatg
     4081 tcactgatac gtacaacaag gtaattaagc gtatctttat gctctgtaaa aataatgagc
     4141 tttctgcggc taccacttgc tgtaaacatc tcgggtttat cttgcaacaa acaggaaagc
     4201 tcttcccatt ttttatcatt tcctgattgc accacactaa gtgcttggta ttctaggtct
     4261 ttaaggctaa gtatttcggc ctcaagctct ggaatggttt cggccgcaga ggcctgatca
     4321 accacttgct caacataaag ttcgtactct gctgcactta aatcctcatc aagttgatct
     4381 aagttctcgg gtaggtctat ctttttctta acgctgtact caccaatggt ttcggcaata
     4441 tggttacttt ttaccgattg gccacgagcc atgagtttgg tttcagcaag tctatcctct
     4501 aaacgcttac gacggcgttt taatgattgg tatatggctt ctgggcttga tgccaagcga
     4561 cgctgcaaca tggttaatgc aaagccgacg ttattctttt tttggccatc cagattatct
     4621 gcccggttca tttcttcacg aacgtaagtg gttacctgtt cgtagagtga tgcttccata
     4681 ggtgatagtt cgtaattggc agtgtaagcg cgcctctctg gaaatagcgg agtgccatca
     4741 aatttaagca gttcttcttt gaccatacga cgcatcatgt cagagatatc gactttatgc
     4801 acaccctcgc ggaatttacc gtaaaaacga tctccatcga gcagtgacaa ccatatttga
     4861 aagtcttctt cttttccgtt gtgtggcgtt gctgtcatga gtaggtaatg acgtgtaatc
     4921 gatccgagca attcgcctag ctggaatcgc ttggttttat tcaccttatt gccaaaataa
     4981 ttggccgaca acttatgagc ctcatcaacg ataattaaat cccaatcggt atttttgagc
     5041 ttttcttgga agtcttcatt gcgagacagt tgatctaagc gacaaataag ttggtcggat
     5101 tcatcaaaat aattaccaga agcgcactgc tcctgttttt cacggctaaa aatatcgaag
     5161 gtgacaccaa acttttctaa caactcatct tgccattgtt cagtcagtga accaggtgac
     5221 acaataagaa tgcgcttggc atctgcacgc atcaataact cacgaatcaa taaacccgcc
     5281 ataatggttt taccagcacc tgggtcatcg gccaatacaa agcgtaaagg ttgcttaggt
     5341 agcatatact cgtaaacagc ggaaatttgg tgaggtaagg gttctacatt ggaggtatgc
     5401 acagccatca tggggtcaaa caatgctgct tgtgtaatac ggtatgcttc taaccctaac
     5461 ttaaactctg cgccaggtgc atcaaatgcc caaggacgac cagccgttgc tagctctaag
     5521 cgagactcat ctgaacggaa taacatctgc tcgccaagct taccttggct gtctttgtag
     5581 taaaccgtga tagcagcatc gccgacaggt tcaacttgaa caatacgcac aatttcttca
     5641 gcttgaatac cgcgaatctg cgcatctttt ttaacatctt cgagttttag catattgtat
     5701 ccctattgtt ctgctgcttg gccagatcgc acccaatcat caacttcg
//
LOCUS       DAOQMB010000117         5740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6135_length_5740_cov_12.238347, whole genome shotgun sequence.
ACCESSION   DAOQMB010000117 DAOQMB010000000
VERSION     DAOQMB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5740
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6135_length_5740_cov_12.238347"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1806
                     /locus_tag="PLY05_05455"
     CDS             <1..1806
                     /locus_tag="PLY05_05455"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNC02785.1"
                     /translation="VPILLWCMLALRYDTLNPPFLPTIPYAFIWLSLFCVSIFYAFGI
                     YRTVVRAFDEKFLQDVVWAVSTIVIVLISAATLNLLPTLPRSATIGFGFLVFLWLWGS
                     RSIIRYMVRLMVQLQAPVTRVVIYGAGVAGRQTLAALRTAPEYFPVAFFDDDSSLINT
                     NVQGLRVYAGKQFAALYKDLEIDEVLIALPSATRARRHEVIELLRPFNIHVRSLPGFN
                     HLVDGKVSISDLREVEIADLLGRDAVPPMPELMAKDITAKCVMVTGAGGSIGSELCRQ
                     IIKVKPSALILWELTEFALYSIEQELKQKAPDIPLIPILGSVLHQERMTHLLQYYQVD
                     TVYHAAAYKHVPLVEWNPFEGIVNNAVGTYRTAKAALTAGVSTFVLVSTDKAVRPTNV
                     MGASKRLSELSLQALAALPDVKTRFCMVRFGNVLGSSGSVVPLFRTQIAKGGPVTVTH
                     PEMTRYFMTIPEASQLVIQAGAMGKGGDVFVLDMGEPVKIFDLAKKMIALSGMTVKEA
                     GKEGDIEIQFSGLRSGEKLYEELLIGSNVVTGTEHPRIMRAVENSLPINILDKLFDDL
                     HDTAQAYDIVKLKELLKQHVEGYVPDMNVIGLMKR"
     gene            1911..2768
                     /gene="galU"
                     /locus_tag="PLY05_05460"
     CDS             1911..2768
                     /gene="galU"
                     /locus_tag="PLY05_05460"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004941729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HNC02786.1"
                     /translation="MKKISSVVLPVAGLGTRFLPATKAIPKEMLPVLDKPLIQLAVEE
                     AAKAGITQVVLVTSASKRAIEDHFDVNFELEATLEARNKQSMLEAVRTTLPNGMTLAV
                     VRQPKALGLGHAVLCAAPVVGDQPFAVMLPDDFIDDPQGSCLADMVALYNQQSAFSLA
                     VEEVPREKTDKYGVVSLNADGRITAMVEKPKPEVAPSTLAVVGRYVLPAETFAALREV
                     KAGAGGEIQLTDGIASLLSTQVFRPHRFKGIRYDCGSKLGYLQANTALGLRDQEVGVA
                     FAKWLSSVI"
     gene            2785..4452
                     /gene="pgi"
                     /locus_tag="PLY05_05465"
     CDS             2785..4452
                     /gene="pgi"
                     /locus_tag="PLY05_05465"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC02787.1"
                     /translation="MINNQKMPRSELPEWLNLQCKAKDMGHLHLRDLANDQERHRALT
                     VKVGPFFLDISKQKLDVDALKKLISLAEAVELPNAIKSLIGGDIVNNTEGRPALHSAL
                     RLPVDAKLIINGEDVVPEVHQSLARIRQIVERVHSRQWRGFSGRAITDVVNIGVGGSD
                     LGPFMACQALNEFVPTEASQLKIHFVSSIDGTQVSDLLGTLKPETTLFIVSSKSFTTI
                     DTLSNAHTAMQWMLKSVGDKSLILAHHFIGVSTQPQKMAAWGIPENNQVLFWEWVGGR
                     FSLWSGIGLPIALKIGMANFEAMLAGAHFIDNEFSQKPLAENLPVLLGLIGVWNATFL
                     DIRAHSVLPYDGRLKFFPSYLSQLEMESNGKSVDKQGNRLNYSTCPVLWGEVGPNAQH
                     AFYQLLHQGTQPVSCDFIAPIKRYTGTIYSKEAEKSLQVQHQLALANCLAQSRVLMLG
                     DASTDSDENTPNFKRYQGNQPSTTLLLDELSPFTLGGLVALYEHKVFVMSVIWDINPF
                     DQWGVELGKKIADKMTNALANGEGIAGFDASTCGLLDLVRQAERSVS"
     gene            4449..>5740
                     /locus_tag="PLY05_05470"
     CDS             4449..>5740
                     /locus_tag="PLY05_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC02788.1"
                     /translation="MKLCVHGDSLFAWVTAAKLAEVGHKVTMRISGINNSFDPERETN
                     LVALLEKQQQAQRLQLVSLADDFEHVMYVHFIAADLHVEGIKELIISIMTHNDDEQAL
                     YLLLVSTLPIGTLDHLRDFVLSKDIELGRKHATYIIGLPLFCREGSALDDFSRPSLLL
                     MSGKEDSHEIMVALEVLRPFVRKAKHMMIVPHRTSELIKMGINAMLATRISFMNEMAA
                     LAEKLGIDIDVVRQGLAADPRIGSDYLQAGCGFGGPSFSEDLLSFAKTLREELDTTGL
                     IDTVLMINDSQREILFRKVWRFFAGRLAGKKIAIWGAAFKAGTASLENSVVHPLLEAL
                     WAQGCYTAVYDPMAAEALYAQYGKHPLLTIAKNAEEVVTDADALVLVTAWDEFLSPDF
                     STIKTLLKQPVVFDGRNIYDPEYMQEQGFRYFAIGRGEA"
BASE COUNT         1692 a          909 c         1284 g         1855 t
ORIGIN      
        1 gtgccaattt tattatggtg catgttagca ttacgttatg acacgttaaa tcctcctttt
       61 ttgccaacta ttccatatgc ctttatatgg ctatcattgt tttgtgtttc aatcttttat
      121 gcatttggta tctatcgtac agttgttcga gcctttgatg aaaagttctt acaagatgtg
      181 gtttgggctg tcagtacaat agtgattgtt ttgattagtg cagcaacact gaatttgttg
      241 cctaccttgc ctcgttcagc aacaattggt tttggctttt tagttttttt atggttatgg
      301 ggaagtcgct ctatcatacg ctatatggta aggttgatgg tgcagttgca agctccagtg
      361 acacgagtcg ttatttatgg agcgggtgta gcaggaagac aaactttagc agctttacgt
      421 acagcacctg aatattttcc tgtcgcattt tttgatgatg actcaagctt aattaatacc
      481 aatgtacaag ggttacgagt atatgcagga aaacagtttg ctgcactata taaagaccta
      541 gagattgatg aggttttaat tgccttacct tcggcaactc gagctcgtcg tcatgaagtg
      601 attgagcttc tacgtccatt taatatacat gttcgctctt tacctggttt taatcattta
      661 gtggacggta aggtttctat ttctgatttg agagaggtag aaattgcaga tttattaggg
      721 agagatgctg ttcctccaat gcccgagctg atggctaaag atattacggc taaatgtgtg
      781 atggtgacag gagctggtgg ctcaattggt tctgaattgt gccgtcaaat aatcaaagtc
      841 aagccttctg ctttaatttt atgggaatta acagagtttg cactttatag tattgaacaa
      901 gagttaaagc aaaaagcacc agatattcct ttaattccca ttttaggctc agtgcttcat
      961 caagagagaa tgacacatct tttgcaatat tatcaagtgg atacagttta tcatgcagcc
     1021 gcatataaac atgttccttt ggttgaatgg aatccatttg aaggcattgt taataatgca
     1081 gttggcactt atcgcacagc aaaagcagca ctaacggctg gcgtttctac gtttgtatta
     1141 gtatcaacgg ataaagcagt cagaccaact aatgtaatgg gggcgtctaa aagattatca
     1201 gaattatctt tacaggctct tgctgcattg cctgatgtaa aaacaagatt ttgtatggtg
     1261 cgttttggca atgttttggg ctcatcaggt tcagtagtac cattatttcg gacacaaatt
     1321 gccaaaggtg gaccagttac agtcacacac cctgaaatga cacgttactt tatgaccatt
     1381 ccagaagcat ctcaattagt gattcaagca ggcgcaatgg gtaaaggagg agatgtgttt
     1441 gtattagata tgggcgaacc tgttaaaatt tttgatttag ccaaaaaaat gattgctttg
     1501 agtggtatga ccgtaaaaga agcaggtaaa gaaggggata tagaaattca gtttagtggc
     1561 ttacgttctg gtgaaaagct ctatgaggag ttattgattg ggagtaatgt tgtgactggt
     1621 acagagcatc cacgaattat gagagcagta gaaaactcat tgccaattaa tatattagat
     1681 aaattatttg atgatttgca tgatactgcg caagcatatg atattgttaa gttaaaagag
     1741 cttttaaaac aacatgttga gggttatgtt cctgatatga atgtgattgg cttgatgaag
     1801 cgttaaacaa aatattatta acatataaat aaaaagcaca actcttgcaa gatgttgtgc
     1861 tttttattta tatagatatc aacgaaataa tgaatcagaa tggagcttct atgaaaaaaa
     1921 tttcctcagt tgttttacct gttgcaggtt taggtacgcg ttttttgcct gcaaccaaag
     1981 caatacctaa agagatgttg cctgttctag ataaaccgtt aattcaatta gctgtagaag
     2041 aggcagctaa agcaggtatt acccaagttg tgttagttac aagtgctagt aaaagagcaa
     2101 ttgaagatca ttttgacgtg aattttgaac tagaagcgac tttagaagca cgaaacaaac
     2161 aatcaatgtt agaggccgta cgcacaactt tacctaatgg tatgacccta gcggtagtac
     2221 gccaacctaa agccttaggt ttgggacatg ctgtgttatg cgctgcacct gttgttggtg
     2281 atcaaccatt tgcagtaatg ttgcctgatg attttattga tgatccccaa ggcagttgct
     2341 tagctgatat ggttgctctt tataatcaac aatcagcatt ttctttagct gttgaagaag
     2401 taccacgaga aaaaacggat aaatatggcg tggtatcctt aaatgctgat ggtcgtatta
     2461 cagccatggt agaaaaacca aaaccagagg ttgcaccaag tacattggct gttgttggtc
     2521 gttatgtgtt gcctgcggaa acatttgctg cattacgaga agttaaagca ggtgctggtg
     2581 gagaaatcca actaacagat ggtattgcta gtttattatc aacacaagta tttcgccctc
     2641 atcgttttaa gggtattcgt tatgattgtg gtagtaaatt aggctattta caagccaata
     2701 cagctttagg tttgagagat caagaggtgg gcgtagcgtt tgctaagtgg ttgagttcgg
     2761 ttatctgatg taaggacact cgttatgata aataatcaaa agatgccgcg ctctgaatta
     2821 ccagaatggc tgaatttaca gtgtaaggca aaagatatgg ggcatttaca cttacgagat
     2881 ttggcgaatg atcaagaaag gcatcgtgct ttgaccgtaa aagtagggcc ttttttttta
     2941 gatatttcaa aacaaaaatt agatgttgat gcccttaaaa agctaattag cctagctgaa
     3001 gccgtagaat taccaaatgc tattaaaagc ctaattggtg gagatattgt taataataca
     3061 gagggacgac ctgctttaca ttcagcattg cgtttgcctg ttgatgcgaa attaatcatt
     3121 aatggtgaag atgttgttcc agaggttcat caaagtttag caagaatacg acaaattgtc
     3181 gaacgagtac attctcggca atggcgtggt ttttctggca gagcgatcac ggatgttgtt
     3241 aatatcggtg ttggtggctc tgatcttggg ccatttatgg cctgtcaagc actcaatgag
     3301 tttgtgccta cagaagcaag tcagctgaaa attcattttg tgagttctat tgatgggact
     3361 caagtatctg atttgctggg tacattaaaa ccagaaacaa cattgtttat tgtttcatct
     3421 aaatcattta ctaccattga taccttatcg aatgctcata cagccatgca atggatgtta
     3481 aaatcggttg gagataaatc tttaatactg gctcatcatt ttattggtgt ttctactcag
     3541 cctcaaaaaa tggcagcatg gggaattcct gaaaataacc aagtattatt ctgggagtgg
     3601 gttggcggtc gtttttcttt gtggtcaggt attggtttac caattgcact aaaaattggt
     3661 atggctaatt ttgaggcaat gttagcaggt gcacacttta tagataatga atttagtcaa
     3721 aagcctttag ctgaaaattt gcctgtatta ttaggattaa ttggtgtatg gaatgcaacg
     3781 tttttagata ttcgtgcgca ttcggtattg ccttatgatg gccgattaaa gttttttccg
     3841 tcttatttat ctcagttaga aatggaaagt aatggtaagt cagtagataa acaaggaaac
     3901 aggttaaatt attctacatg tcctgtgcta tggggagaag tcgggccaaa tgcccaacat
     3961 gctttttatc aattattgca tcaaggtaca cagccagttt catgtgactt tattgcacca
     4021 attaagcgat atacggggac tatttattca aaggaagcag aaaaatcttt acaagttcaa
     4081 catcaattgg ctttagctaa ctgcttagct caatcaagag ttttaatgct tggtgatgca
     4141 agcacggata gtgatgaaaa tacgcctaat tttaaacggt atcagggaaa tcaaccatca
     4201 accacgttat tacttgatga actcagtcca tttactttgg gtggattggt tgcgctttat
     4261 gaacataaag tatttgttat gtcagttatt tgggatatta atccctttga tcaatggggg
     4321 gttgagttag gtaaaaaaat cgctgataaa atgactaatg ctcttgccaa tggagaaggt
     4381 attgctggct ttgatgcttc tacatgtggc ttattagatt tggttcgtca agcagaaagg
     4441 agtgtttcat gaaattatgt gtacatggtg actctttgtt tgcatgggta acagccgcta
     4501 aattagcaga agttggccac aaagtaacaa tgcgtattag tggtattaat aatagttttg
     4561 accctgagcg tgaaacaaat ttagtggctt tgttagaaaa gcaacaacaa gcacaacgct
     4621 tacaattagt atcgttagcg gatgattttg agcatgttat gtatgtgcat tttattgccg
     4681 ctgatttgca tgtcgagggt attaaagaac tcattatcag tatcatgact cataatgatg
     4741 atgaacaagc cctttattta cttttagttt ctaccttgcc cataggtact ttagatcatt
     4801 tacgagattt tgtattatca aaagatattg agttagggcg caaacacgct acatatatta
     4861 taggtttgcc attattttgt cgcgagggaa gtgctttaga cgacttttca cgaccctcgt
     4921 tattattaat gagtgggaaa gaagatagtc atgaaataat ggttgcctta gaggtactta
     4981 gaccttttgt gcgtaaagcc aaacatatga tgattgtgcc gcatcgtaca tctgagctta
     5041 taaaaatggg tattaatgcg atgttagcaa cacgcattag ttttatgaat gaaatggctg
     5101 cacttgctga aaagttaggt atagatattg atgtcgtacg tcagggtttg gcagctgacc
     5161 cacgtattgg aagtgattat ttacaagctg gttgtggttt tggtggccca agcttttcag
     5221 aagatttatt gagttttgca aaaacgttgc gtgaagagtt ggacacaacg ggacttattg
     5281 ataccgttct gatgattaat gactcccaaa gagaaatttt atttagaaaa gtatggcggt
     5341 tttttgcagg gcgtttagcg ggtaaaaaaa tagcgatttg gggagctgca tttaaagcgg
     5401 gtacagcaag tttggaaaat tcagtcgtcc atcctttgtt agaggcttta tgggcgcagg
     5461 gatgttatac cgctgtttat gatcctatgg cagccgaagc gttatatgca cagtatggta
     5521 aacatccatt attaacgatt gctaaaaatg ctgaagaagt agttactgat gctgatgcct
     5581 tagtattggt gacagcatgg gatgagtttt tgagccccga cttttcaact ataaaaacat
     5641 tgttaaaaca acctgttgtt tttgatggtc gtaatattta tgacccagaa tatatgcaag
     5701 agcaaggatt tagatatttt gctattggtc gtggtgaagc
//
LOCUS       DAOQMB010000118         5715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6186_length_5715_cov_16.667138, whole genome shotgun sequence.
ACCESSION   DAOQMB010000118 DAOQMB010000000
VERSION     DAOQMB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6186_length_5715_cov_16.667138"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..1514
                     /locus_tag="PLY05_05475"
     CDS             276..1514
                     /locus_tag="PLY05_05475"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HNC02789.1"
                     /translation="MKTYLVGGAVRDTLLGKTVKDRDWVIVGATPEQLLQLGYTAVGK
                     DFPVFLHPHSHEEYALARTERKIGSGYHGFSCETGSHISLEQDLARRDLTINALAQDE
                     NGQIIDPYGGQADLTAKILRHVSPAFAEDPLRVLRVARFAARYADDGFSIAPETLDLM
                     QQIVQQGELAHLSAERIWLETHKALGEARPDIYIEVLQQCGALQALFPEIAQLFGVPQ
                     RAEYHPEVDTGIHILMCLRVAARDHLSTRARFAVLVHDLGKGITPKEQLPRHINHEHT
                     GVALVTALCERYKVPRDYQQLAEMVCQYHLDCHRVVELQAKTLWGKLQKLDAIRRPER
                     FIEFLQACQADAQGRLGLEHKPYPQFDYFRQAQQVVAQITPKQLANLSQLNGAEIGHA
                     LGALRIHALHEFKKQYLQQS"
     gene            1480..2259
                     /locus_tag="PLY05_05480"
     CDS             1480..2259
                     /locus_tag="PLY05_05480"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011280638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HNC02790.1"
                     /translation="MSLKNNTFNNPKVVLVTGSGQRVGAEIVRSLHQSGWTVLIHYRH
                     SQQAALSLANELNQQRANSAYTLRADLDDLNQINQLSQQIEQITDRLDALVNNASTFY
                     PTPIGNVDDNAWHDLFSSNAKAPFFLSQACVPLLKKTQGCIVNIVDIHAQRPFKNYTV
                     YCMAKAALQTMTYALAKELAPDIRVNGVAPGAILWPEMNSPSAINNDTQAEILAGIPL
                     KRSGTPQDIAKTVRFLVEDAPYITGQIIAVDGGRSHTLGME"
     gene            complement(2298..2672)
                     /gene="folB"
                     /locus_tag="PLY05_05485"
     CDS             complement(2298..2672)
                     /gene="folB"
                     /locus_tag="PLY05_05485"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HNC02791.1"
                     /translation="MDILYIRNLKVDAIVGIFSWEKRIRQPIHLDLEIAIDIRQAAST
                     DDIAYALDYKTVSTRLTNYISQSEWHLIETLAEKVASLLMDEFGVTWLRLRLSKPAAL
                     PAADSVGLVIERGSQQIRPLFG"
     gene            complement(2796..3728)
                     /locus_tag="PLY05_05490"
     CDS             complement(2796..3728)
                     /locus_tag="PLY05_05490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC02792.1"
                     /translation="MNTSVSNNIGHTTAAPATKKLALGATVNIPPRRLDFEFSTDSER
                     YYYDNNPYLSSFLTTLSSLFPAGESFFVEAVRHYRERITDPVLKAQVAGFIGQEAMHS
                     KEHQGFNDMATRQGYPVDKLDRDVTKLLDLVQKTLPKPVQLAATVALEHYTAIIAEML
                     LRDADVQQKFSVEARHLWLWHALEENEHKTVAYDVYQQMVGSYGLRVATMALTTVIFF
                     AVTFAFHTRMLAADGQLFKFRQTWQAMKYLWNGKKGVFSRLLPQYLDFFKPSFHPKDH
                     DTDALLAQWKEILFAKEDGVLYEQLKNKSAVGVH"
     gene            3855..4865
                     /locus_tag="PLY05_05495"
     CDS             3855..4865
                     /locus_tag="PLY05_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HNC02793.1"
                     /translation="MSINLQSLPSVYALLLVDVAANWQVKPEQLLADLGLTREMLLEP
                     QRRISLEDVNKLIIKARQLTQEAALAIYFGQKMQISMHGFIGFAALTAANTREALTIA
                     ERFVGMISSLVELRVEEGAEKSNLILTINTQLQPLRELAVLAVMFGFAHMGYAATGQV
                     LTGDVEVDFACPDYLEPLIPFLPAKVSFNSQINQIVFSSTYLDLPLIMANPVASQLAL
                     TQCEQELQRFGLDKPFVAQVKALMFDEKMGFSTLEQVAQRLHMSERTLKRQLAKYELT
                     YTDLVEEARKQKALDLLTNSQLSLDDISEYLGYSDLANFTRAFKRWTEQTPTAYRKSL
                     AK"
     gene            5093..5572
                     /locus_tag="PLY05_05500"
     CDS             5093..5572
                     /locus_tag="PLY05_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016659595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HNC02794.1"
                     /translation="MIIEVTGDLLLSKAQVIAHGIAPNDNFGQGLALSLREQWPSLYK
                     DFRHFCQTQHPKEGTIWSWMSSEGKVIVNLLTQEGAYHHGEKPGRANTKHVHHALAEL
                     RKLIDSENFSSVALPKLATGVGGLSWEEVYPLIQQHLGDLSIPVYVYTTYHKGQQAE"
BASE COUNT         1748 a         1170 c         1115 g         1682 t
ORIGIN      
        1 tattacccgt accctcgatg ttatccgtac ctgttaatat caggtcatca accccttcag
       61 gtaaagtata agaaacatcg gaagtatgtg ttgtgcgaga aatggccatg tgtagatact
      121 cctaatctgt gggtcagcag gttccatgct taggcaagta acagccgccc gctaaatttt
      181 tatgacggta cggttaaaga caagttggta aactaaatat attgagtata tcctattctg
      241 ctgttgtatt tttagacttt tttttggcaa acacaatgaa aacatattta gtgggtggtg
      301 cagtccgcga taccctctta ggcaaaaccg ttaaagaccg tgattgggtg attgtaggtg
      361 ctacacctga gcaattacta caactcggct ataccgctgt tggtaaagat tttcctgtgt
      421 ttctgcatcc acacagccac gaagaatatg ccctagctcg aactgaacgt aaaatcggtt
      481 cgggctatca tggttttagc tgtgaaacag gcagccatat tagtttagaa caagatttag
      541 cccgccgcga cctaacaatt aatgcccttg ctcaagatga aaatggacaa attatagacc
      601 cctatggtgg tcaagccgat ttaacggcaa aaattttacg ccacgtctcg cctgcttttg
      661 ccgaagaccc tttacgtgtg ttaagagtcg ctcggtttgc cgcacgttat gctgatgatg
      721 gctttagtat tgcccccgaa accttagacc ttatgcagca aattgtgcaa caaggagaat
      781 tagcgcattt atcagccgaa cgtatttggc tagaaacgca taaagcacta ggagaagcgc
      841 gacccgatat ttacattgaa gttttacaac aatgtggggc attacaagcg ttgttccctg
      901 agattgccca attatttggt gtacctcaac gcgctgaata tcaccccgaa gtggatacag
      961 gtattcatat tttaatgtgc ttacgggttg ctgctcgcga ccatttatca acacgcgcgc
     1021 gttttgcggt attagtccat gacttaggca aaggcattac ccctaaagag caattgccac
     1081 gacacattaa tcatgaacat acaggcgttg ctttagtcac tgccttatgt gaacgctata
     1141 aagtgccacg cgattatcaa caattagccg aaatggtttg ccaatatcat ttagattgtc
     1201 atcgggttgt tgagttgcaa gctaaaacat tatggggcaa gctacaaaag ctagatgcta
     1261 ttcggcggcc agagcgtttt atcgaatttt tacaggcttg ccaagcagat gctcaagggc
     1321 gtttaggttt agaacataaa ccctatccgc aatttgacta ttttagacaa gcccaacaag
     1381 ttgtagcaca aattacccct aaacagctag ccaatttaag ccaactcaat ggcgcagaga
     1441 taggtcatgc tttaggtgct ttacggatac acgcattaca tgagtttaaa aaacaatacc
     1501 ttcaacaatc ctaaagtggt tttagtgaca ggcagtggtc aacgggtggg tgcagagatt
     1561 gttcgctctt tgcatcaatc gggctggaca gtattaattc attatcgtca tagtcaacaa
     1621 gcggcattaa gcttagccaa tgagttaaac caacaacgtg caaacagtgc ttatacctta
     1681 cgtgccgatt tagatgattt aaatcaaatt aatcaattat cacaacaaat tgagcaaatc
     1741 actgatcgtt tagatgcttt agttaataat gcgtcaacat tttatcccac accaattggc
     1801 aatgttgacg ataatgcgtg gcatgatttg tttagtagta acgccaaagc accatttttt
     1861 ttgagtcaag cctgtgtgcc tttactgaaa aaaacgcaag gctgtattgt caatattgtt
     1921 gatattcatg ctcaacgccc ctttaaaaat tatactgtgt actgtatggc taaagcggcg
     1981 ttacaaacca tgacttatgc tttagctaaa gagcttgcgc ctgatattcg ggtcaatggt
     2041 gtggcaccag gtgcgatttt atggccagaa atgaacagcc cttcggcgat taataacgat
     2101 actcaagcag aaattttggc aggtattccc ttaaaacgct caggcacacc ccaagatatt
     2161 gctaaaacgg tacggttttt agtggaagat gccccatata ttacaggtca aattattgcc
     2221 gttgatggtg gtagaagtca taccttaggg atggaataaa aaaaggctta gcaatctaag
     2281 cctttttatt tcataattta accaaacaat ggtcgaattt gttggctgcc tctctcaatc
     2341 accaagccga cactatccgc agcgggcaaa gccgcaggtt tagacaaacg tagacgtaac
     2401 cacgttacgc caaactcgtc cattaataaa ctcgccactt tctcggctaa agtttcaatt
     2461 aaatgccatt cactctgact aatataattg gtcaaacgtg ttgagactgt tttgtaatca
     2521 agggcgtagg caatgtcatc cgtcgatgct gcttgacgaa tatctatcgc aatttctaaa
     2581 tctagatgaa taggttggcg aatacgtttt tcccacgaaa agataccgac aatggcatca
     2641 acttttaaat tacggatata taaaatgtcc acaactttag cctgtataaa aaaattaggc
     2701 tgtattataa acaaaaaagg gcgcaaaaat gcgccctgtt ttatgtgcca attattaaac
     2761 gttaaccacc tccgtttgaa ccactggcgc ataaattaat gcacaccaac ggcagatttg
     2821 ttttttaatt gctcatataa cacgccgtct tctttggcaa ataaaatctc tttccattgt
     2881 gccaataaag catcggtatc atgatcttta ggatgaaagc ttggcttaaa gaagtctaaa
     2941 tactgtggca acaaccgtga aaacacgcct tttttgccat tccacaagta tttcatggcc
     3001 tgccaagttt gacgaaactt aaataattgg ccatccgctg ctaacatgcg tgtatgaaag
     3061 gcaaacgtta ccgcaaagaa aatgacagtg gttaaagcca tcgttgcaac acgcaaacca
     3121 taactaccaa ccatttgttg ataaacatca taagccactg ttttatgctc attttcttct
     3181 aaagcatgcc acaaccataa atgacgtgct tcaaccgaaa atttttgctg aacatctgca
     3241 tcacgcaaca acatttctgc aataatcgcg gtatagtgct ctaacgctac tgtagcggct
     3301 aactgaacag gcttaggtaa ggttttttgt actaaatcta ataatttagt cacatcacga
     3361 tcaagtttat ctacaggata accttgtcgg gtagccatat cattaaaccc ctgatgctct
     3421 ttgctgtgca tggcttcttg gccaataaaa cctgcaactt gggcttttaa cacagggtca
     3481 gtaatacgct cacgataatg gcgtactgct tcaacaaaaa atgattcacc tgcaggaaat
     3541 aaagacgata atgtcgtcaa aaaggaactt aaataagggt tgttatcgta ataataacgc
     3601 tcagaatccg tcgaaaactc aaaatctaag cgacgtggtg ggatatttac cgttgcacct
     3661 aatgccaact ttttagttgc aggagctgcc gttgtatggc caatattgtt agaaacgcta
     3721 gtattcatat gcaacctctt aatttgtggt gctacttcat ttttacattg actaatgtca
     3781 cactcataat gtaattgtat ggttgtacgg taagtaggtg cttttgtgcc atttttttat
     3841 cattagagga caatatgtct atcaatttgc aaagtttgcc tagcgtctat gccttgttat
     3901 tagttgacgt tgctgctaac tggcaagtaa agcccgaaca gttattggcc gatttaggct
     3961 taaccagaga aatgttgcta gaaccacagc gacgtatttc tttagaagat gtcaacaaat
     4021 tgattattaa agcgcgtcaa ctcacccaag aggcggcatt agcgatttat tttggccaaa
     4081 aaatgcaaat ttcgatgcat ggttttattg gttttgccgc cttaacagcc gctaatacac
     4141 gagaagcact aactatcgct gaacgttttg tgggcatgat tagtagcttg gttgaattac
     4201 gtgttgaaga aggcgcagaa aaaagtaatc tgattttaac cattaatacc caattacagc
     4261 ctttgcgtga attagccgtg ttagccgtga tgtttggttt tgctcacatg ggttatgcag
     4321 caacaggcca agtattaaca ggtgatgttg aagttgactt tgcttgccct gattatttag
     4381 agcctttgat tcctttttta cctgccaaag tcagctttaa tagccaaata aatcaaattg
     4441 tcttttccag cacttattta gatttaccac tgataatggc taatccagtt gccagtcaat
     4501 tagccttaac ccaatgtgaa caggaactac aacgctttgg tttagacaaa ccctttgtag
     4561 cacaagtaaa agccttaatg tttgatgaaa aaatgggctt ttcgactctc gaacaagtcg
     4621 ctcaacgttt acatatgtcc gaacgcaccc taaaacgcca attagccaaa tatgaattga
     4681 cttataccga tttagtagaa gaagcacgaa aacaaaaagc cttagattta ttaaccaaca
     4741 gccaactgag tttagatgat atttccgaat atttaggtta ctccgattta gccaacttta
     4801 cccgtgcctt taaacgctgg acagagcaaa caccaaccgc ttaccgcaaa agcctagcaa
     4861 aataatcaac tattaagaga gtcttttttc aattcgtaaa tgtaaaattt atttatataa
     4921 caacacgtta aataactaaa aaacctgcta gacaagcaaa ttttataggc atattatcaa
     4981 aatcataaac catatatttt atgtggtttt aaaccccttt gggagttagg caattaatac
     5041 ccagtaggct agcctaactc ccctttttat tgatatggca aagagaaaac ttatgattat
     5101 tgaagtgact ggtgatttgt tattaagtaa agcccaagta attgctcatg gtattgcacc
     5161 taatgacaac tttggtcaag gtttggcttt atcattacgc gaacaatggc caagcttata
     5221 caaagacttt cgtcattttt gccaaacaca acaccctaaa gaaggcacaa tttggtcatg
     5281 gatgagttct gaaggtaaag tcattgtcaa tctattaacc caagaaggtg cttatcatca
     5341 cggtgagaaa ccgggtcgtg ctaacactaa acatgtacat catgccttag ccgaattacg
     5401 taaattgatt gacagcgaaa attttagcag cgtggcttta cctaaattag ccacaggtgt
     5461 aggtggttta tcgtgggaag aagtttatcc cctcattcaa caacacttag gtgatcttag
     5521 tattcctgtg tatgtgtata cgacttatca taaaggccaa caagctgagt gattgaaaaa
     5581 gagcgcgata ccgcgctctt ttttattgtg gcaacaagcc tcttagctgt ttattcaaat
     5641 agtcttgata attataaatg ggaaatggaa taggctcatt agactttgca tattcttctt
     5701 tttttaccat acgca
//
LOCUS       DAOQMB010000119         5657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6303_length_5657_cov_13.517672, whole genome shotgun sequence.
ACCESSION   DAOQMB010000119 DAOQMB010000000
VERSION     DAOQMB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6303_length_5657_cov_13.517672"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLY05_05505"
     CDS             <1..501
                     /locus_tag="PLY05_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017389123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC02795.1"
                     /translation="NFWGVVNGCEAFLPQLRQQKDAHIVNISSIFGIIGVPSQSAYNA
                     SKFAVKGFTEALRQELNGSNIHVTCVHPGGIKTNIARNARVHNDMFGKAADPKKLAAD
                     FDKLAATTAEEAARQIVRAIENNQKRLLIGKDAKALDAIQRLLPNHYDTVLGVGYKLM
                     NKLAKR"
     gene            complement(558..1223)
                     /locus_tag="PLY05_05510"
     CDS             complement(558..1223)
                     /locus_tag="PLY05_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC02796.1"
                     /translation="MSYMQLSREGTVWVLTLTNGENRLNDDVLAEFNTALDTIENTEG
                     NGAVVITSADPKFWSNGIDLNFVQEKGGMAYLVKEFAPRLDQLLMRIARFPLPVIACL
                     NGHAYAGGALIAAACDFRTMRADRGRFCFPEVDIKIPFTPIMTEIVRLLPNPTIAWQM
                     ASTGLALGGEEAAKAGVVDKALGEAELLPTTMAWAAELAKKDRQTYTLIKRRWRNAFD
                     KFI"
     gene            complement(1392..>2245)
                     /locus_tag="PLY05_05515"
     CDS             complement(1392..>2245)
                     /locus_tag="PLY05_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC02797.1"
                     /translation="TRQSLLFSATYPDAIHTMSQKILQTPVVVTVESVDETNHIEQHF
                     VEVAYSDKAIAVTKILAHYRPESTVIFCNTKADCNELTQHLRSKGFDVLTLHGDLEQR
                     DREQVLLRFANKSCPVLVATDVAARGLDIKELSAVINFELAYDPEVHVHRIGRTGRAG
                     NQGLAFSLCAPKEAPRVVAIEEYQKIKVNWLDLNELHTPVVASLKATMVTICIEGGRK
                     DKLRPTDILGALTGEGGLAGADVGKIDIFDIRAYVAVKSHVAPKALKRLQAGQIKGRR
                     FRVTKVG"
     assembly_gap    2246..2345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2534..2890)
                     /locus_tag="PLY05_05520"
     CDS             complement(2534..2890)
                     /locus_tag="PLY05_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC02798.1"
                     /translation="MYHIVVRLLLSACGLGLADYLLEGIRFDHFSTLFWAALLLGVAN
                     SIVKPILIFFTLPITLLTLGFFLLVINAAILGLVAALLSSFHVSGFWSALAAWFIVAL
                     TQAVGSVLLKSKKSDS"
     gene            complement(2900..4615)
                     /locus_tag="PLY05_05525"
     CDS             complement(2900..4615)
                     /locus_tag="PLY05_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004962829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02799.1"
                     /translation="MKQATALNILKAGHNVFLTGSAGAGKTYTLNQYIHYLRARKVPV
                     AMTASTGIAATHMNGMTIHAWSGIGIKDSLDDDELTRMFERKYLRSQIDKAEILIIDE
                     ISMLHARQLDMVNQVLKFFRNTNEAFGGLQVVVAGDFFQLPPVGKNQESNRDKFAFMS
                     TAWQEAKFNICYLTEQHRQQNNSLNTILNEIRAQQVSTESVQLLRQTRQQKLEADITR
                     LFTHNVDVEQINQRYMNGINQKSYVFNAEVKGNEKLVQTLISNVRAPQQLVLKLGAKV
                     MFVKNNMESGYINGTLGEVVDFIEEDGQTLPKVKLRDGTHLYVTPETWSIDNDSAKPL
                     ASYSQIPLCLAWAITVHKSQGMTLEAAEIDLSQTFEQGQGYVALSRLKTLDGLRLLGL
                     NDVALQLDSLALKADKRFLELSNQAEQIWVDKDASQLHKDFIRRCGGSIDVDEIAKYE
                     KRQQKIANNKSSEPHTIEQTKVLFLQGMSIVEIAKERELTQSTIINHLSKIYDKEPSF
                     DLSRIKPDDKVLAKVSHAFHQLQQEDNDEYFNEDGTMKLRPIYEALKQELGYNDIRLA
                     LVFVA"
     gene            4790..5614
                     /locus_tag="PLY05_05530"
     CDS             4790..5614
                     /locus_tag="PLY05_05530"
                     /inference="COORDINATES: protein motif:HMM:NF019829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNC02800.1"
                     /translation="MKLNAKNLILDLLLASNDSPLSVRDAIAACRLFGLSDNSVRVAL
                     ARASADGLIEAAGRGTYRLSASALQLAGEVATWRTAEQRIRPWQGGYIAVLTQHLGRT
                     DRAALRKRERALAMLGFASLETGFYLRPDNIDENLNHIRQRLCRLGLEAQALVFYANQ
                     FSEQEQTRISQLWNGAALNQQYRQLQQQMADWLAHAPSLEPDVAARESFLLGSKAIRQ
                     VVFDPLLPEPWVDVAERHRFVESVHAFDQAGHLIWRKLIEVSLTPTPSHKEREFSE"
BASE COUNT         1674 a         1206 c         1014 g         1663 t
ORIGIN      
        1 aatttttggg gcgtggtgaa tggttgtgaa gcctttttac cgcaattacg ccaacaaaaa
       61 gatgcacata ttgttaatat ttccagtatt tttggcatta ttggtgtgcc gtcacaatct
      121 gcttataatg ccagtaagtt tgcggtaaag ggctttaccg aagcgttacg tcaagaatta
      181 aatggcagca atattcatgt cacttgtgtg catcctggtg gcattaaaac caatattgcc
      241 cgtaatgcgc gtgtgcataa tgatatgttt ggcaaagcgg ctgacccaaa aaaattagcg
      301 gctgattttg ataaattagc agcaaccacc gccgaagaag cggccagaca aattgttcgt
      361 gctatcgaaa acaaccaaaa acgtttgtta attggcaaag atgccaaagc cttagatgcg
      421 attcaacgtt tattaccgaa tcattacgac accgttttgg gtgtaggcta taagctaatg
      481 aataaactgg ctaaacgcta aaaaattatt taacaccacc aaaaacaccc cacgctcact
      541 aaacgtgggg atttaattta aataaactta tcaaatgcat tacgccagcg acgcttaatt
      601 aaggtataag tttgacgatc tttcttggct aactcggcag cccatgccat cgttgttggc
      661 aataattcag cttcgcccaa agccttatca accacacctg ctttagccgc ttcttcacca
      721 cctaaagcca aaccagtact tgccatttgc catgcaatcg ttggatttgg taataaacgc
      781 acaatttcgg tcataatggg cgtaaatgga attttgatgt ccacttctgg aaaacaaaaa
      841 cgaccacgat cggcgcgcat ggttctaaag tcacaagcag ccgcaattaa tgcaccacct
      901 gcataagcat gaccatttaa acaggcaata acaggtaaag gaaaacgtgc aatacgcatt
      961 aacaactgat ctaaacgtgg tgcaaactct ttgaccaaat aggccatgcc acctttttct
     1021 tgcacaaagt ttaaatcaat accattactc caaaactttg gatcagcact ggtaatgacg
     1081 actgcaccat tgccttcggt gttttcaatg gtatctaatg cggtattaaa ctccgccaat
     1141 acatcgtcat ttaaacgatt ttcaccatta gttaaggtta atacccaaac agtaccttca
     1201 cggcttaatt gcatataact catgttttcc cccactatat tgcgactata aacaaatacg
     1261 ctgtcattgc gtaaacggct gttgagctta aacaagcata tgcttggttg cagctgtttt
     1321 ttttgactac tagttttcac ttactcgaac ttagacagtt attgctcatg cctaagcctt
     1381 aaacattgtt gctaaccgac ttttgtcacc ctaaaacggc gtcccttaat ttgtcctgcc
     1441 tgtaagcgtt ttaaggcttt aggggcaaca tgacttttta ctgccacata agcacgaata
     1501 tcaaagatgt caattttacc tacgtctgca cctgccaaac caccctcacc tgttaatgct
     1561 cctaaaatat ctgtgggtcg caatttatct tttcgaccac cttcaataca aatcgtcacc
     1621 atcgttgctt ttaatgaggc aaccacaggg gtatgcaact catttaagtc taaccagttc
     1681 actttaattt tctgatattc ttcaatagca acaactcgtg gtgcttcttt gggcgcacac
     1741 aaactaaaag ctaaaccttg attacctgcg cgacccgttc taccaattcg gtgaacatgc
     1801 acttcgggat cataagccag ctcaaaatta atcactgcac ttaattcttt aatatccaaa
     1861 ccgcgtgcag cgacatctgt ggccactaac acaggacaac ttttattggc aaaacgtaat
     1921 aacacttgct cacgatcacg ttgctctaaa tcaccatgca aggtcaacac atcaaaacct
     1981 ttactgcgta gatgttgtgt taactcgtta caatcggcct tggtattaca aaaaatcacc
     2041 gttgattctg gtcgataatg tgccaaaatt ttggtgactg ctattgcctt gtcactataa
     2101 gcgacctcaa caaaatgctg ctcaatatgg ttcgtttcat caaccgattc tacagtgacc
     2161 acaacaggcg tttgcagaat tttctggctc atggtgtgaa tggcatctgg gtaagttgcc
     2221 gaaaataaga gactttgcct tgttgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnctgcc cgttttcgct ttggctaata catctttgcc tgctaacatg ggtggtaaac
     2401 tttgctgttg aatagaagtc atttgttgat aacccaaaga ctcgacactt tgtaataaaa
     2461 caggcaataa cggtaaagag ctaaactcag cagtagtcat aaaacactca aataatttag
     2521 aagcaaaaag aaattaactg tcggatttct tagatttcaa caacacgcta ccaacggctt
     2581 gtgttaatgc cacaataaac caagccgcta aagctgacca aaaaccactg acatgaaagc
     2641 ttgataataa agctgctacc aagcccaaaa tggccgcatt aattactaat aaaaagaaac
     2701 ctaaggttaa taaggtaatt ggcaaggtaa aaaagattaa aatcggttta acaatgctat
     2761 tggccacacc taataacaaa gctgcccaaa acaaagtgct aaagtggtca aaacgaatgc
     2821 cttctaacaa ataatcggct aaacctaaac cacaagcact caataataat ctaacaacaa
     2881 tgtgatacat aaaactctct taggccacaa acactaatgc taatcgaata tcattgtaac
     2941 ctagctcttg tttaagtgct tcataaatag gtcgcagctt cattgtgcca tcttcattaa
     3001 aatactcatc attatcttct tgttgcaatt gatgaaaagc atgactgact ttggccaaaa
     3061 ccttatcatc aggcttgata cggcttaaat caaagctggg ctctttgtca taaatttttg
     3121 ataaatgatt aataatcgtc gattgcgtta actcacgctc tttggcaatt tccacaatac
     3181 tcatgccttg taaaaacaag actttagttt gttcaatggt atgtggctca gaacttttgt
     3241 tattggcaat tttttgctga cgtttttcat atttagcaat ttcatcaaca tcaatactgc
     3301 caccacaacg tctaataaaa tctttatgta attggcttgc gtctttgtct acccaaattt
     3361 gttcggcttg attggataat tctaaaaaac gtttatccgc ttttagagcc aaactatcca
     3421 attgtaaagc aacatcattt aagcccaaca aacgtaaacc atccaaggtt tttaaccgtg
     3481 acaacgccac ataaccttga ccttgctcaa aggtttgact taaatcaatt tcagccgctt
     3541 ctaaagtcat gccttggctt ttgtgtacgg taatcgccca cgccaaacac aagggaattt
     3601 ggctataact ggctaatggt ttagcactgt cattatcaat cgaccatgtt tcgggcgtaa
     3661 catataaatg cgtgccatct cgtaatttga ctttgggtaa ggtttgacca tcttcttcaa
     3721 taaaatcaac aacctcacct aaagtgccat taatataacc actttccata ttgtttttaa
     3781 caaacatcac ttttgcaccc aattttagaa ctaattgttg gggtgcacga acgttgctaa
     3841 tcaaggtttg tactaacttt tcgttgcctt tgacttcggc attaaataca taggactttt
     3901 gattaatacc attcatataa cgttggttaa tttgttcaac atcaacatta tgggtaaaca
     3961 aacgggtaat atcagcttct aatttttgct gacgggtttg ccttaataat tgcacacttt
     4021 ctgttgaaac ttgttgggcg cgtatttcat taagaatggt atttaagcta ttgttttgtt
     4081 ggcgatgctg ctcagttaaa taacaaatat taaacttggc ctcttgccat gctgtcgaca
     4141 taaaggcaaa tttatcacga ttgctttctt ggtttttccc tactggcggt aattgaaaaa
     4201 aatcaccagc gaccaccact tgtaaaccgc caaacgcctc attggtattt ctaaaaaatt
     4261 ttaagacttg attgaccatg tccaattggc gagcatgcag catggaaatt tcatcaataa
     4321 ttaaaatttc ggctttatct atttggctac gcaaatattt tctttcaaac attcgtgtta
     4381 attcgtcgtc atccaaactg tctttaatac caatacctga ccacgcatga atcgtcatgc
     4441 cattcatgtg agtggccgca atccctgttg aagcggtcat ggcaacgggt actttacgag
     4501 cacgtaaata gtgaatgtat tgattaagcg tataggtttt tcctgcacca gcagagcctg
     4561 tcaaaaagac attatgccca gctttgagga tatttaatgc ggttgcttgt ttcatacaaa
     4621 gtaactaatt ctaaaactga ttaactgcat gatagcttaa aggattttat agctcgattg
     4681 ttgattttca gaaatcaaaa cgacattttt aaaattacaa acaattgcat tagcatttaa
     4741 tttatgtaat attacataaa atatcaatat atttactttg atgtaacaaa tgaaactcaa
     4801 tgccaaaaac ttaatccttg atttattgct tgcctcaaac gactcgccat tatcagtacg
     4861 tgacgcgatt gccgcctgtc gattgtttgg tctaagtgat aacagtgtac gggtggcttt
     4921 ggcgcgtgcc tctgccgatg gtttgattga agcagcaggt cgtggcactt atcgtttaag
     4981 tgcatcggca ttacaactgg caggtgaagt cgcgacatgg cgcaccgctg agcaacgtat
     5041 taggccgtgg caaggtggct atattgccgt tttaacgcaa catttaggcc gcaccgaccg
     5101 tgctgcctta cgcaaacgtg aacgcgcttt agcgatgttg ggttttgcga gtttagaaac
     5161 aggcttttat ttgcggcctg ataatattga tgaaaatttg aatcacattc gtcaacgttt
     5221 atgtcgttta ggtttagaag cacaagccct cgttttttat gccaatcagt ttagcgaaca
     5281 agaacaaaca cgcattagcc aattgtggaa tggtgctgcg cttaatcaac aatatcgcca
     5341 attacaacaa caaatggcag attggttagc ccatgcgcca agtttagaac ctgatgtggc
     5401 cgcacgagag tcttttttac tgggtagtaa agccattcgc caagtggtgt ttgacccgtt
     5461 attacctgag ccgtgggtcg atgtcgctga gcgtcatcgt tttgttgaat cagtacatgc
     5521 ttttgaccaa gcaggccatt tgatttggcg caaattgatt gaggtatccc tcaccccgac
     5581 cccctcccac aaggagaggg agttttcaga gtgagcctaa ataattcagg agcagttaaa
     5641 atgtctgcat ctccccc
//
LOCUS       DAOQMB010000120         5576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6468_length_5576_cov_9.846405, whole genome shotgun sequence.
ACCESSION   DAOQMB010000120 DAOQMB010000000
VERSION     DAOQMB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6468_length_5576_cov_9.846405"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="PLY05_05535"
     CDS             complement(<1..580)
                     /locus_tag="PLY05_05535"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNC02801.1"
                     /translation="MVYYIDPNTGREYSIDPSKRGAVTEPLITGIGNRFDIEEDYLAD
                     DEIGVKVKMNTLKDKYEGCLLGLACGDAVGTTVEFKPRGSFAPITDMVGGGPFDLDVG
                     QWTDDTSMALCLAESLLAKNGFDAKDQMDRYLRWCKEGYMSSTGHCFDIGRTISGALR
                     NYSLNKNPYAGSTEPRTAGNGSIMRLAAIPLYY"
     gene            complement(701..>2717)
                     /locus_tag="PLY05_05540"
     CDS             complement(701..>2717)
                     /locus_tag="PLY05_05540"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HNC02802.1"
                     /translation="STQELTFWLRKQQQDHHINYMNLNATTPNEKNKTEEEQEKELEK
                     ELEKELEKELEKELEKAKKQAERDKSRKSLLSRAEECMTDTQLQKMFKNLAWMLDFHE
                     REAKPTWWRLFDRLGLTEDELFDDLECLALCQRTQEAPFKPTPRARNLAYQYTFDTNQ
                     EFKGVAKNFYVLGEYSDKGKELKVSCLKEHSDLKNGVITVQCSQALPQLMTLIPDDFV
                     NPEPIPLALKNVIEDICKGSQQHLAIQQFLARKRPVIDGHTNNDIVVATEPQQRLNEI
                     IKVVENLQNSYLVIQGPPGAGKSYTAKHIIAELLKSGKKIGISSNSHKAINHLLLTTM
                     QYCQHQGIKAHFVYTKEDEALESQQAKRVENAQLHNQIQNACVLGTTAWGFAREDMTN
                     QLDYLFVDEAGQVAVANLIAMSRSAKNLILMGDQMQLGQPLQGTHPLESGSSILDYLL
                     RDQATIDNKMGVFLSESYRMHSAVNQYISDYIYDGKLHAHPDNDKQLIHLPNDYQGEL
                     NKQAGVVFVPVHHQGNTQASDEEVNKIKSLVDELLTRTLTTKEGETRQVTWQDILFIA
                     PYNHQVRKLQDELGSQAKVGSVDKFQGQEAPIVILSMCTSDAAEAPRGLDFLFNKNRL
                     NVAVSRAKSLAIIIASPQLQITKVNSVEQLKKVNLFCGLVQERAIKD"
     assembly_gap    2718..2817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2818..4350)
                     /locus_tag="PLY05_05545"
     CDS             complement(<2818..4350)
                     /locus_tag="PLY05_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HNC02803.1"
                     /translation="MQKQNNGQYLYSPSDLVRFVDSPFASKMERLLCEGEISRELKNK
                     QTQDAELLTEGGFAHERKFEKKLRQQNKKVTKIVGSTLQEKQDNTIKAMQAGDEVIVQ
                     GYLSHLPFAGYADFLIKVSGASKLGDYHYEVWDTKLAHRVKPEYLIQLCCYAEMLKEI
                     QGVLPEYLTIALGNDQNERIKTIDCFAYYQAIKAAFLQEQNGFDKTQKPHPADYKEWG
                     QWSDYAKNLLVEEDHLFQVANIRYQQIQKLNQAGIKTLAELAKTNLQGIKGINQKAFE
                     RLQQQAELQEKAKAGELAFRIIKPNQGDLTGLALLPPHSASDVYFDIEGFPFEKDGLE
                     YLWGVTYFEGDKRCFKCYWAHDQEQEKKAFEDFITWVYQRWQNDKSMHIYHYASYEIT
                     ACRKLMGRFGVCEYEVDELLRHGVFVDLYQIVRNGLLAGLPKYSIKDLEPLYNFKREG
                     EVANGGDSIVAYDEYRKLWFDGQQEQARLKLDAIKAYNQKDCDSTQELTFWLRKQQQD
                     HHI"
     gene            complement(4356..5372)
                     /locus_tag="PLY05_05550"
     CDS             complement(4356..5372)
                     /locus_tag="PLY05_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNC02804.1"
                     /translation="MPANNSRHTLARQWELLKLLPNNTHGKTVNELVDGLNKLDFKIG
                     HRQVERDLKMLEETLALRCDSNVVPYRWSLLRKVNYAMSLSEAVSWQLVAKTIKNLLP
                     ANILHEVAPYFEQAQKKLDALKEENNAAAAWTEKIRVVEPTLPLVAPTIDITILENIQ
                     ESVLKQIQIEVIYRAANSSLSKELTLHPLALVQRGLVTYLVATVNDYVDIRIFAIHRI
                     VNIWLTQRSAIIPSNFDIDKYIATGALHFGNGEQIHLKLRVANWLKNILQETPLTTNQ
                     EISEQDNNIIVKAVITLTPQLEWWLLSQADGLEVLEPIELREKLAKRLLLAAEQYQAN
                     TTKG"
BASE COUNT         1566 a         1148 c          857 g         1905 t
ORIGIN      
        1 aataatacaa gggaatggca gctaaacgca taattgaacc gttgcctgct gtccgcggtt
       61 ctgttgaacc tgcataagga tttttattta aagaatagtt tcttaacgca cctgatattg
      121 ttctgccaat gtcaaagcaa tggcctgtac tactcatata gccttccttg caccatctaa
      181 gatatctgtc catttggtct tttgcgtcaa aaccgttttt agccaataaa ctctctgcca
      241 aacacaatgc cattgatgta tcatcagtcc attgccccac atctaagtca aaagggcctc
      301 cgccaaccat atccgttatg ggtgcaaaac tccctcttgg tttaaactcc actgttgtac
      361 ccactgcatc accgcaagct aatcctaata aacacccttc gtatttgtct tttaatgtat
      421 tcattttaac cttaacacct atttcatcgt cagctagata atcttcttct atgtcgaaac
      481 gattaccaat accagtaata agtggttcag tgacagcacc acgtttgcta gggtcaatac
      541 tgtattcacg tccagtatta gggtcgatgt aatataccat aatcacctca attattgtcc
      601 caaagacact agtttgattt tagtcgattt ttaaagcaat ttcaatatca taaaacaaac
      661 aacgaccgct tagtcgttgt tggctttatg aaaataatcg ttaatctttt attgctcgtt
      721 cctgcactaa tccacaaaat aaattaacct ttttaagttg ctcaacagag tttactttgg
      781 tgatttgcag ttggggactt gcaataataa ttgctaaact tttcgctctt gaaacggcaa
      841 catttaagcg atttttatta aacaaaaagt ctaagcctct aggtgcttcg gctgcatcac
      901 ttgtacacat acttaaaatt acaatcggag cttcttgccc ctgaaattta tcaacactac
      961 ccaccttcgc ttgtgagcca agctcatctt gcagctttct gacttgatga ttataaggag
     1021 caataaacaa tatatcttgc caagtgactt ggcgtgtttc gccttctttg gtggtgagtg
     1081 ttcttgttaa tagctcatca actaatgact taattttatt aacctcctca tcagaggctt
     1141 gtgtattgcc ttgatgatgg actggcacaa atacaacacc tgcttgttta tttaattcac
     1201 cttgataatc attaggtaaa tgtatcaatt gcttgtcatt atctgggtga gcatgcaatt
     1261 ttccatcgta aatatagtca ctaatatact gattaaccgc actgtgcata cggtaacttt
     1321 ctgataaaaa cacgcccatt ttgttatcta tagtagcttg gtcacgcagt aaataatcta
     1381 aaatagaaga accactctcc agtgggtgtg ttccttgtaa aggttgcccc aactgcattt
     1441 ggtcgcccat taaaatcaag tttttagcac tacgactcat ggcgattaaa ttagcaacag
     1501 ctacctgacc agcctcatca acaaacaaat aatccagttg gtttgtcata tcctcacgag
     1561 caaaacccca agcagtcgtt cctaatacac aagcattttg aatttgatta tgaagctgcg
     1621 cattttccac acgcttcgct tgttgactct caagagcttc atcctcttta gtgtaaacaa
     1681 aatgcgcttt aatgccttga tgttgacaat attgcatcgt tgttagtaac aaatggttaa
     1741 tagctttgtg actattacta ctaataccaa tttttttacc cgatttgagt agctcggcaa
     1801 taatatgctt tgccgtataa gactttcctg cacctggcgg tccttgaatc actaaataac
     1861 tattctgtaa attttcaaca accttaataa tttcatttag tcgctgctgt ggttctgtcg
     1921 ctactacaat atcattattg gtatgcccat caataacagg tctttttcta gctaaaaact
     1981 gttgtatggc taaatgctgt tgactgccct tacaaatatc ttcaatgaca ttttttaatg
     2041 ctaatgggat aggctctgga ttaacaaaat catctggaat tagagtcatc agttgcggca
     2101 atgcttgact gcactgcaca gtaatcacgc cattttttaa atctgagtgt tcttttaaac
     2161 aacttacctt tagttctttt cctttatctg aatattctcc caaaacgtaa aaatttttgg
     2221 cgactccttt aaactcttga tttgtatcaa atgtatattg atatgctaaa tttctagctc
     2281 taggggtagg tttaaatgga gcttcttggg tacgttgaca taatgctaaa cattccaaat
     2341 catcaaaaag ctcgtcctcg gttaagccta aacggtcaaa cagtctccac catgttggct
     2401 ttgcttcacg ctcatgaaaa tccaacatcc atgctaaatt cttaaacatt ttttgtaatt
     2461 gtgtatcggt catacattct tctgcacgag ataaaagcga cttgcgtgac ttatctcgtt
     2521 ccgcttgttt tttagctttt tccaactctt tttccaactc tttttccaac tctttttcca
     2581 actctttttc caactctttt tcttgttctt cttcggtttt gtttttttca tttggtgtcg
     2641 ttgcatttag attcatatag ttaatatgat ggtcttgttg ctgtttacgc aaccaaaatg
     2701 tcagctcttg cgtagagnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     2821 atgatggtct tgttgctgtt tacgcaacca aaatgtcagc tcttgcgtag agtcacagtc
     2881 tttttggtta tacgctttaa tagcgtcaag ttttaacctc gcttgctctt gttgaccatc
     2941 aaaccataac tttctatatt catcataggc aacaatcgaa tctcctccat tagcaacttc
     3001 gccctctcgc ttaaagttgt acaatggttc taaatcttta attgaatatt tgggaagccc
     3061 tgctaataag ccatttctta caatttggta taaatcaaca aacacaccgt gacgtaataa
     3121 ctcatcaact tcatattcac atacgccaaa acgccccatt aactttctac atgcggtaat
     3181 ttcgtaagat gcgtaatggt aaatatgcat gcttttatca ttctgccaac gctgataaac
     3241 ccatgtaata aaatcttcaa aagccttttt ttcttgctct tggtcatgcg cccaataaca
     3301 tttaaaacaa cgtttatcac cttcaaaata ggtaactccc cataaatact ctaggccatc
     3361 tttttcaaag ggaaaacctt caatatcaaa atagacatct gatgcagagt gtggcggcaa
     3421 taaggccaaa ccagttaagt caccttgatt aggcttaata atacgaaaag ccaactcacc
     3481 agcttttgct ttttcttgta attctgcttg ttgctgtaaa cgctcaaagg ctttttgatt
     3541 aatcccttta ataccttgta aatttgtctt agctaattcg gccaacgttt taatacctgc
     3601 ttgattgagt ttttgaattt gctggtatct aatattagct acttgaaata gatggtcttc
     3661 ttcgactagt aaatttttag cataatcaga ccattgcccc cattctttgt aatcagcagg
     3721 atgcggcttt tgtgttttat caaatccatt ttgctcttgt aaaaacgccg ctttaattgc
     3781 ttgataatag gcaaaacaat ctattgtttt aatacgctcg ttttggtcat tacctaaagc
     3841 aatcgtcaag tattcaggta atactccctg aatctctttg agcatttctg cataacaaca
     3901 taactgaatt aaatattcag gtttaacacg atgagccagt ttggtatccc aaacttcata
     3961 gtgataatcg ccaagcttac ttgcacctga gactttaatt aaaaaatcag cataccctgc
     4021 aaacggtaaa tgcgataaat aaccttgaac aatgacctca tcacctgctt gcatggcttt
     4081 tatggtgtta tcttgtttct cttgcaaagt actccctaca attttagtca cttttttgtt
     4141 ttgctgacgc aatttttttt caaattttct ttcgtgcgca aagccacctt ctgttaaaag
     4201 ctcagcgtct tgtgtctgtt tattttttaa ttctctggaa atttctcctt cacataacaa
     4261 tcgctccatt ttagaagcaa aaggagaatc aacaaaacgc actaaatcgg aaggcgaata
     4321 caaatattgg ccattatttt gtttttgcat attttttaac ctttagtagt gtttgcttga
     4381 tattgctctg ctgctaacaa taaacgctta gccagctttt ctcttagctc aataggctct
     4441 aatacttcta ggccatccgc ttgagataat aaccaccatt ctaattgtgg ggtcaaggta
     4501 attaccgcct taacaataat gttattatct tgctcactaa tttcttgatt agtggttaag
     4561 ggggtctctt gtaaaatatt ttttaaccaa ttagctactc gcagttttaa gtgaatttgt
     4621 tcgccattgc caaagtgtaa tgcacctgtg gcaatatatt tatcaatatc gaaattgctt
     4681 ggaataatcg cactacgttg ggttagccat atattcacta ttcgatgtat agcaaatatc
     4741 ctaatatcca catagtcatt tacggtcgca acaagataag tcactaaacc tcgctgcacc
     4801 aaggctaaag gatgtagagt caactcttta ctcaaggatg aatttgctgc ccgataaata
     4861 acttcaattt gtatttgttt taaaacgctt tcttgaatat tttccaaaat agtgatgtct
     4921 attgttggag caaccaatgg caacgttggc tcaactacac gaattttttc ggtccatgct
     4981 gctgctgcgt tattctcttc ttttaaagca tctaactttt tttgtgcttg ctcaaaatat
     5041 ggcgcgactt catgtaagat attagctggt agtaaatttt tgatagtttt tgcaacaagt
     5101 tgccaagata ctgcctcaga taaagacatg gcataattta cttttctgag caatgaccag
     5161 cgatatggca caacattact atcacatcgc aatgctagcg tctcttccag catttttaaa
     5221 tcacgctcta cttgccgatg acctatttta aaatcaagtt tatttaagcc atcaactaat
     5281 tcattaactg ttttgccatg tgtattgttt ggcagtagtt ttaataactc ccattgacgt
     5341 gcaagtgtat gacgtgaatt attagcaggc atgtttcacc ttaaaacaaa gtattgagta
     5401 ttttctatca atatctttta tgattttaag aaattagcta taactcagct aatttcttat
     5461 tatacgtatt tctatcacat ctaaactcta tcttctagca tgccaaatct aaatacgtca
     5521 cattgtgtcg cattgaataa cattttttaa ttatcttgat aacaagcatc agaata
//
LOCUS       DAOQMB010000121         5572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6479_length_5572_cov_15.558456, whole genome shotgun sequence.
ACCESSION   DAOQMB010000121 DAOQMB010000000
VERSION     DAOQMB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6479_length_5572_cov_15.558456"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLY05_05555"
     CDS             complement(<1..497)
                     /locus_tag="PLY05_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNC02805.1"
                     /translation="MAITIAVDAMGGDFGPRVTVPAALSVLARYPDLKIILVGQETQI
                     SPHLQKSSTIIKQRVSIVHTDEMVTMDDKIAVALRNKRQSSMRLAINLVHDKQANACV
                     SAGNTGALMAISRFVLKTHANIDRPAILAPLPTQTGQVYMLDLGANVDSEPKHLVQFA
                     QMGNLV"
     gene            complement(506..691)
                     /gene="rpmF"
                     /locus_tag="PLY05_05560"
     CDS             complement(506..691)
                     /gene="rpmF"
                     /locus_tag="PLY05_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNC02806.1"
                     /translation="MAVQQNRKTRSRRDMRRSHDALTGPTLSVDQTTGEVHRRHHVTK
                     DGFYRGRQLFTVAAVDA"
     gene            complement(746..1291)
                     /locus_tag="PLY05_05565"
     CDS             complement(746..1291)
                     /locus_tag="PLY05_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HNC02807.1"
                     /translation="MINPYYRAPIMPVVSLPAAIEPFKWADRATHVVVDVPLAQFTRL
                     ATDLVDTNGSVHIDCRFERNAQRRAYLRGEISTVINLTCQRCLDTMQLPIQTTIDLAL
                     VHDEDEAEKLPEDADYLVVPEQGVVLAEIIEDELLLNVPYTPMHEDCDALSYKQDDVA
                     APVKENPFQVLAALKKPSAKD"
     gene            complement(1398..2906)
                     /locus_tag="PLY05_05570"
     CDS             complement(1398..2906)
                     /locus_tag="PLY05_05570"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNC02808.1"
                     /translation="MTVIKQDDVIQSVADALQYISYYHPLDFIQAINNAYEKEENQAA
                     KDAMAQILINSRMCAEGHRPICQDTGIVTVFVKVGMNVRWDATMSLEDMINEGVRRAY
                     LHPDNVLRASILKDPAGKRQNTKDNTPAVIHYEIVAGDKVDITVAAKGGGSENKSKMA
                     MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKESLMDPVDIQELI
                     ARGAQNRVEELRLELFEKVNALGIGAQGLGGLTTVVDIKIRDYPTHAASLPVAIIPNC
                     AATRHVHFELDGSGAAELTPPKLEDWPQVTWDASKARRVNLDNISHEDIKTWQTGETL
                     LLSGTIYTGRDAAHKRMADMIAKGEELPVDLKGKFIYYVGPVDPVRDEVVGPAGPTTA
                     TRMDKFTNTVLEATGLYGMIGKSERGPVAIQAIKNHEAVYLMAVGGAAYLVSKAIRKA
                     KVVAFADLGMEAIYEFVVEDMPVSVAVDVNGSSVHDTAPKIWQAKIGKIPVV"
     gene            complement(2951..>3175)
                     /locus_tag="PLY05_05575"
     CDS             complement(2951..>3175)
                     /locus_tag="PLY05_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02809.1"
                     /translation="VRNLEKIDFWQFYQGLPWPLPQPKDLDNLHDDDHWARALAPRLL
                     AVHNYQQQQFSLPLAQILAIQDATVDNNSL"
     assembly_gap    3176..3275
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3438..4025
                     /gene="wrbA"
                     /locus_tag="PLY05_05580"
     CDS             3438..4025
                     /gene="wrbA"
                     /locus_tag="PLY05_05580"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HNC02810.1"
                     /translation="MPEPYILVLYYSRHGNTRQLAKLIARGVEAAGMTARIRTVPAVS
                     TVCESTEAAIPDSGELYCSLEDLRDCAGLALGSPTRFGNMAAPMKYFLDGTAGLWLNG
                     ALCDKPAAVFTSTSSLHGGQESTLLSMMLPLLHHGMVILGLPYREAALNSTQSGGTPY
                     GASHWAGQDGKRPITAEEQALCLALGKRLVQFARR"
     gene            complement(4059..4154)
                     /gene="ffs"
                     /locus_tag="PLY05_05585"
     ncRNA           complement(4059..4154)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLY05_05585"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4233..4730
                     /locus_tag="PLY05_05590"
     CDS             4233..4730
                     /locus_tag="PLY05_05590"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNC02811.1"
                     /translation="MDIFFLSHLNSPLGLIHFIHKDNVLYALEFDEFHHRLTQSLTRY
                     YPTDFAIHVCETSIIHVLLDTYFSGDLDALNRIQVNALGTNFQQQVWQSLRTIPAGKT
                     ASYGQIAQQIGNKNGQRAVGMANGKNPIPLVIPCHRVINHNGKLGGYSGALWRKEWLL
                     KHEQI"
     gene            5007..>5572
                     /locus_tag="PLY05_05595"
     CDS             5007..>5572
                     /locus_tag="PLY05_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004702813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC02812.1"
                     /translation="MVVTTLKNATLGKLSLRKHIKISDYVSTGTMPIRHFNFNFDAKT
                     FDDKFYLQKEWASAYFYALSIFLTYGEELVIETARYHRDFITDPILKQRVSALIGQEA
                     LHSKFHNEYNDIMTEHNFPVKLYRFLAEKVFEYTFLKFPQPLKLSMMAGIEHFTAVVA
                     EYMMKHEDHFYYSDDAKARALWMWHMLE"
BASE COUNT         1587 a         1166 c         1085 g         1634 t
ORIGIN      
        1 accaaattac ccatttgggc aaattgcact aaatgtttgg gttctgaatc aacatttgcc
       61 cctaaatcaa gcatatacac ttgaccagtt tgggtaggca acggagctaa aatagcagga
      121 cggtctatat tggcatgcgt ttttaataca aaacggctaa tagccattaa cgcacctgta
      181 ttacctgcac tcacacaagc attagcctgt ttgtcatgca ccaaattaat tgccaaacgc
      241 atcgacgatt gacgtttatt acgcaaagca accgcaattt tatcatccat tgtcaccatt
      301 tcatcggtgt gaacaataga tacgcgttgt tttatgatag tagatgattt ttgtaggtgt
      361 ggcgaaattt gtgtttcttg accaaccaga atgattttta aatcaggata gcgtgctaga
      421 acgcttaaag cagcaggaac agtaacacgg ggaccgaagt ccccgcccat ggcatcaacc
      481 gcgatggtga tagccatact ataacttagg cgtcaaccgc agcaacagta aacaattgac
      541 gaccacggta gaaaccgtct ttagtcacat ggtgacgacg gtgaacttca ccagtggttt
      601 ggtcaacgct taaagttggg ccagttaatg catcatggga acgacgcata tcgcgacgag
      661 aacgagtttt acgattttgt tgaacggcca ttttagctct ccaaacacac aagactaaac
      721 atcgtaacga cgtttatgga ttagattaat ctttcgctga cggctttttt aatgctgcca
      781 acacctgaaa tgggttttct ttgacgggtg cagcaacgtc atcttgttta taacttaatg
      841 catcacaatc ttcatgcatt ggtgtataag ggacgtttaa caacaactca tcttcaataa
      901 tttctgccaa cactactccc tgttctggca caactaaata gtctgcatct tctgggagtt
      961 tctcagcttc gtcttcatca tgcactaagg caaggtcaat cgtggtttga atcgggagtt
     1021 gcattgtgtc taaacaacgc tgacaagtga gattaatcac cgtcgaaatc tcgccacgta
     1081 aataagccct tctttgagca ttacgctcaa accgacaatc aatgtgtacg ctaccattag
     1141 tatcaactaa atcggtagct aaccgtgtaa attgcgccaa aggaacatcg actacaacat
     1201 gtgtagctct atctgcccat ttaaagggtt caatggcggc tggtaatgag acgactggca
     1261 taataggggc gcgataataa gggttaatca atagtttgtc tagttttttt aaatttccct
     1321 cccctaattt aggagagggt cagggtgggg tcatttataa ccccctccca acctccccct
     1381 tatcaaggga gaggagctta gacaactgga attttaccaa ttttggcttg ccaaattttg
     1441 ggtgcggtat catgcactga gctgccgttg acatctaccg ccaccgagac aggcatatct
     1501 tcaacgacaa actcataaat cgcttccata cctaagtctg caaaagcaac gactttggct
     1561 ttacggatag ctttggatac taaataagca gcaccaccca ccgccattaa atacacagct
     1621 tcatggtttt taatggcttg aatagcgact ggaccacgtt cagatttacc aatcataccg
     1681 tataagcctg tggcttctaa tactgtatta gtaaacttat ccatacgggt tgcagttgtt
     1741 gggcctgctg ggccaactac ttcatcacga acgggatcaa ctggaccaac gtaataaata
     1801 aacttgcctt ttaaatcgac aggtaattct tcacctttgg caatcatatc agccatacgt
     1861 ttatgggcag catcacggcc agtatagatt gtgccagaca acaacaaggt ttcaccagtt
     1921 tgccatgttt taatgtcttc atggctaata ttatctaagt taacacgacg cgcttttgac
     1981 gcatcccatg tcacttgtgg ccaatcttct aatttaggtg gagttaattc tgctgcacct
     2041 gaaccatcta attcaaaatg gacatgacga gtcgctgcac agtttggaat aatggcaaca
     2101 ggtaagctgg ctgcgtgtgt tggataatct ctgattttga tatcaacaac cgtggttaaa
     2161 ccacccaagc cttgagcacc aatgcctaaa gcgttgactt tttcaaacaa ctctaaacgt
     2221 aattcttcaa ctcgattttg tgcgccacgc gcgattaact cctgaatatc cacagggtcc
     2281 attaacgatt ctttggctaa taacatggct ttttcggctg taccaccaat accaatccct
     2341 aacatacctg gtggacacca gccagccccc atggttggta cggtttttaa tacccaatcc
     2401 acaatcgaat cagaagggtt taacatcgcc attttcgatt tattttcaga accaccgccc
     2461 ttcgctgcaa cggtaatatc aactttatca cctgcgacaa tttcgtagtg aatcacagct
     2521 ggcgtgttgt ctttggtatt ttggcgttta cctgcggggt cttttagaat agaagcacgc
     2581 aagacattgt cggggtgtaa ataagcacga cggacacctt cattaatcat gtcttctaag
     2641 gacatggtag cgtcccaacg gacattcatg cccactttaa caaacacagt gacaatgcct
     2701 gtatcttggc aaatcggacg atgaccttcg gcacacatac gagaattaat taaaatttga
     2761 gccattgcat ctttggcggc ttggttttct tctttttcgt aagcgttatt aattgcttga
     2821 ataaaatcta aaggatgata ataagaaatg tattgtaaag catcggcaac actttgaata
     2881 acatcatctt gtttaataac agtcatgctg attcctctgt aactggcgtg agctggtttt
     2941 gttggcgcga ttataacgaa ttattatcta ctgtagcgtc ttgaatcgct aaaatttgtg
     3001 ctaatggcaa actaaattgc tgttgttgat aattatgcac ggctaataaa cgcggcgcta
     3061 aagctcgtgc ccaatggtca tcatcatgta aattatctaa atctttaggc tgtggtaaag
     3121 gccacggtaa accttgataa aactgccaaa aatcaatttt ttctaaatta cgcacnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacaag gtagtatagg ttaaggaggc
     3301 cgcattttgt cgacattcat ccgccaaaaa gcgactaatt acaaaacggg taaatgattt
     3361 gattttgtgc caatgactct gcaacgtcat atttcactcc aattggggta taatcctaac
     3421 aaaatgagga taagttaatg cctgaaccct atattttagt actgtactat agtcgtcatg
     3481 gtaacacacg ccaactggca aagttgattg cgcgtggtgt agaagccgca ggcatgactg
     3541 cgcgtattcg tacagtgcca gcagtatcaa ctgtctgtga aagtaccgaa gctgctattc
     3601 ctgatagtgg tgaattatat tgcagcttag aagatttacg tgattgcgca ggtttagctt
     3661 taggcagtcc cactcgattt ggcaatatgg ccgcacccat gaagtatttt ttagatggta
     3721 cagcaggatt atggttgaat ggtgcgttgt gtgataaacc agccgccgtt ttcacctcaa
     3781 ccagttcttt acatggtggt caagaaagca ccttattaag catgatgttg ccattgttac
     3841 atcatggcat ggtcatttta ggtttgcctt atcgtgaggc ggcattaaat agtacgcaaa
     3901 gtggcggaac accttatggt gcatcacatt gggcaggaca agatggtaaa cgcccaatca
     3961 ccgctgaaga acaggcttta tgtttagctt taggtaaacg attagtacaa tttgcgagac
     4021 gctaactaga cattagctga gaagtttagt ggtggcaacc ttgtcagctc accctcggca
     4081 cacgcatcac aactaccgtt gctaccttcc ggtcctggcg gggtttgctg cttagcgttg
     4141 cgaggaaacc gacaaggcca ccataacgcg acacttgatg aatatcaagt gggcggcaag
     4201 tatatgacat tatttagaat tttggaacag ttatggatat atttttttta agtcatttaa
     4261 atagtccttt aggcttgatt cacttcatac acaaagataa tgttttatat gcacttgagt
     4321 ttgacgagtt tcatcatcga ctcactcaat ccttaactcg ttattatcca acagactttg
     4381 caattcatgt ctgtgaaacc agtattattc acgttttgtt agatacttat ttttcaggcg
     4441 atcttgatgc gctcaatcgt attcaagtta atgcgttagg gacaaacttt caacaacaag
     4501 tttggcaaag tttacgtact atccccgctg gcaaaaccgc ttcttatggt caaattgcac
     4561 aacagattgg caacaaaaat ggtcaacgtg cagtaggcat ggctaatggt aaaaacccta
     4621 ttcccttagt cattccttgt catcgtgtca ttaatcataa tggcaaatta ggcggctaca
     4681 gtggcgcttt atggcgtaaa gagtggttac ttaagcatga gcaaatttag aaggtgaacc
     4741 catcacctcc aataataaca tctcaacagc ctcaatcttt gagagcttca atgacacttt
     4801 ttataaaaaa gatataattg catctctcaa tctgtcccca aatcactttt ttttgtcctc
     4861 taatcacctt ttaccgtacg atcgtaatgt aatttaatga agtgtcagat acacacctct
     4921 gtcttactga aatatgtata gataaccacc catattcaaa gactagattt attaactaat
     4981 cattattgcg attttaggta tctatcatgg tcgtcactac attaaaaaat gctactctgg
     5041 gaaaattatc tttgcgtaaa catatcaaaa tttctgatta tgtgagcact ggcacgatgc
     5101 ccattcgcca tttcaacttt aattttgatg caaaaacctt tgatgataaa ttttatttgc
     5161 aaaaagaatg ggcaagtgct tatttttatg ccttatcaat ttttttaact tatggcgaag
     5221 agttagttat tgaaaccgct cgttatcacc gtgactttat tactgaccct attttaaaac
     5281 aacgtgtctc tgcattgatt ggacaagaag ctttacattc taaatttcat aatgaataca
     5341 atgacattat gactgagcat aacttccccg taaaactgta ccgtttttta gctgaaaaag
     5401 tctttgaata cactttttta aaatttccac aaccactgaa gttatcgatg atggcaggga
     5461 ttgagcattt taccgcagtg gttgcggaat atatgatgaa gcacgaagac catttttatt
     5521 attctgacga tgccaaagcg cgtgctttgt ggatgtggca tatgttagaa ga
//
LOCUS       DAOQMB010000122         5558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6512_length_5558_cov_15.829548, whole genome shotgun sequence.
ACCESSION   DAOQMB010000122 DAOQMB010000000
VERSION     DAOQMB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_5558_cov_15.829548"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLY05_05600"
     CDS             <1..318
                     /locus_tag="PLY05_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC02813.1"
                     /translation="KLPDDPLLHQAALVFASDFALMGTAMLPHAVTFMQSNLQAASLD
                     HSIWFHRPFRMDEWLLYEMDAPNANAGRGLNFGRIYSQDGRLVASTAQEGLMRLREVP
                     EKY"
     gene            complement(401..1273)
                     /locus_tag="PLY05_05605"
     CDS             complement(401..1273)
                     /locus_tag="PLY05_05605"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC02814.1"
                     /translation="MKLAFIGLGNMGYPMAGHLANKGYEIIVYNRTTVKAIQWAKEYN
                     TSYATTIPQAVDNADIIFTCVGNDDDLSQVVLAEDGVIHHAKDGAILVDHSTVSAEVT
                     RDLANLLAQRNVSLVDAPVSGGQLGAQKGQLSVMCGAKTEIFAQVEPILQAYGKTIIH
                     MGDIGSGQLTKMVNQICVAGLLQGLAEGIHFAQQSGLDVDKAMSVISQGAASSWQMVN
                     RYPTMLKNEYNFGFAVDWMRKDLDICLAEAAKNNTQLPVTDLVNQYYKDVQNLGGGRW
                     DTSSLLVRLQQDKN"
     gene            complement(1330..2610)
                     /gene="gltA"
                     /locus_tag="PLY05_05610"
     CDS             complement(1330..2610)
                     /gene="gltA"
                     /locus_tag="PLY05_05610"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNC02815.1"
                     /translation="MTGKTATLTVGDKSINLPIYSGTLGPDVIDVKDVLSNGYFTYDP
                     GFMATSACESKITFIDGDKGILLHRGYPIDQLAEKSDYLETCYLLLNGELPNAEQKVA
                     FEQLISSHTMVHEQLQFFYRGFRRDAHPMAVMCGVVGALSAFYHDNLDINNPEHRHIA
                     AIRLIAKMPTIAAMSYKYSMGQPFMYPRNDLSYSENFLHMMFATPCEEYKVNPIIAKA
                     MDRIFVLHADHEQNASTSTVRLTGSTGANPYACIAAGIAALWGPSHGGANEAVLKMLD
                     EIGDVSKLDDFIARVKDKNDGTKLMGFGHRVYKNFDPRAKVMKQTCDEVLTALGINDP
                     QLELAMKLEEIALNDEYFVKRKLYPNVDFYSGIILKAIGIPTSMFTVIFALGRTPGWI
                     AHWLEMHSAAYKIGRPRQLYTGATQRDFVPVEQR"
     assembly_gap    3115..3214
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3356..3751
                     /gene="sdhC"
                     /locus_tag="PLY05_05615"
     CDS             3356..3751
                     /gene="sdhC"
                     /locus_tag="PLY05_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HNC02816.1"
                     /translation="MKSNRPVNLSLGMVLEVNMKSPVAMASILHRISGIVVFLLIPVL
                     LYILQGSLASEESFNYVKDTILGGFIGRFVLFVALAGLIYHFFAGIKHLVMDFGIAEE
                     LKSGRAFASAILAISAFFIALAFYWVVLS"
     gene            3748..4113
                     /gene="sdhD"
                     /locus_tag="PLY05_05620"
     CDS             3748..4113
                     /gene="sdhD"
                     /locus_tag="PLY05_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HNC02817.1"
                     /translation="MKSATSFSRSGMSDWLVQRVSAVILAVYFVVIVGWLLTAGQVTY
                     DAWHSFMTCTAMRIFSLLALLSLAGHCWVGLWTVTTDYMTTRQMGASANFLRVITQIA
                     MAIAIFVYVVWGAIILWGN"
     gene            4117..>5558
                     /gene="sdhA"
                     /locus_tag="PLY05_05625"
     CDS             4117..>5558
                     /gene="sdhA"
                     /locus_tag="PLY05_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004908357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HNC02818.1"
                     /translation="MSHSNIRTLSFDAVIVGGGGSGMRASLQLARAGLSVAVLTKVFP
                     TRSHTVAAQGGIGASLGNMSEDNWHYHFFDTVKGSDYLGDQDAIEFMCREAPKMVYEL
                     EHMGMPFDRNEDGTIYQRPFGGHTSNYGEKPVQRACAAADRTGHALLHTLYQQNLRAK
                     TKFFVEWIALDLIRDEEGDVLGVTAIDLETGEVAVFQAKCTLFATGGAGRIYAASTNA
                     FINTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLLTEGCRGEGGLLRNKNGERFM
                     ERYAPNLKDLAPRDFVSRSMDQEIKEGRGCGPNGDYVVLDLTHLGVETIMKRLPSVYE
                     IGKKFANVDCTKEPIPVVPTIHYQMGGIPTNINGQVVVPKDGNPNTVVKGLYAIGECS
                     CVSVHGANRLGTNSLLDLIVFGKAAGEHIIDTVRRAPHEFKPLPKGVLDVTLARIKKL
                     DDSTSGEYAQDIANDLRKIMQAHAGVFRTQA"
BASE COUNT         1474 a         1072 c         1186 g         1726 t
ORIGIN      
        1 aaattgcctg acgatccttt gttacatcaa gcagccttag tctttgcttc tgattttgcc
       61 ttaatgggca cagccatgtt gccacatgcg gtgacattta tgcagtctaa tttacaagca
      121 gccagtttag accattcgat ttggtttcat cgtccgttta gaatggatga gtggttgttg
      181 tatgaaatgg atgcacccaa tgcgaatgcg ggtagaggtt taaactttgg tcgcatttat
      241 agtcaagatg gtcgtttagt ggcaagtacg gctcaagagg gtttaatgcg tttgcgtgaa
      301 gtgccagaga agtattaacg ctaataaatt aggacttacg cggttatcgc ttatttgttc
      361 gtatttcgat gaaatgcgta agtcctatac ttcaaaccga tcaattctta tcttgttgca
      421 atctcactaa caaactggat gtatcccaac gtccgccacc tagattttgt acgtctttat
      481 aatattgatt aacaagatca gtcacaggca attgtgtatt atttttagct gcttcggcca
      541 aacaaatatc taaatcttta cgcatccaat cgactgcaaa gccaaagtta tattcattct
      601 ttaacattgt gggataacga ttaaccattt gccacgatga ggctgcacct tgactaatca
      661 cactcatcgc tttatcgaca tctaagccag attgttgcgc aaaatgaatg ccttctgcca
      721 aaccttgtaa taaacctgcg acacaaattt gattaaccat tttggtgagt tggccactgc
      781 caatatcgcc catatgaatg atggttttac cataagcctg taagatgggt tcaacctgag
      841 caaaaatctc tgtttttgca ccacacatca cacttaattg acctttttgt gcgcctagct
      901 gtccaccaga gacaggtgca tcaaccaaac tgacattacg ttgagcaaga agattagcta
      961 aatcacgagt cacttctgcc gacacagtag agtggtctac caaaattgcg ccatctttgg
     1021 catgatgaat gacgccatct tcggccaaaa cgacttggct taaatcatca tcattaccca
     1081 cacaagtaaa aataatatca gcattgtcaa cggcttgtgg gatagttgtt gcgtaactcg
     1141 tgttatattc ttttgcccat tgtatggctt taacagtggt acggttataa acaataattt
     1201 catagccttt attggctaaa tgtccagcca taggataacc catatttcct aagccaataa
     1261 atgcaagctt cattttatta cgctcctaaa aaataaaggc cgctgttgcg gcctttatct
     1321 tcgttaaaat taacgttgtt cgactggcac aaaatcgcgt tgtgttgcgc cagtatataa
     1381 ctgacgtgga cgaccgattt tgtaagcagc agagtgcatt tctaaccagt gagcgatcca
     1441 acctggtgtg cgacctaaag caaaaataac ggtaaacata gaagttggaa taccgatagc
     1501 ttttaagatg ataccagagt agaagtctac gtttggatag agtttacgct tcacgaagta
     1561 ttcgtcgttt aaggcgattt cttcgagttt catggctaat tctaattgtg gatcgttgat
     1621 gccaagagca gtcaacactt cgtcacaagt ttgtttcatc actttggcgc gtgggtcaaa
     1681 gtttttgtaa acgcggtgac cgaagcccat taatttagtg ccgtcatttt tgtcttttac
     1741 gcgagcaatg aagtcatcta atttagagac atcaccgatt tcgtctaaca tttttaacac
     1801 agcttcgttt gcaccaccgt gagaaggacc ccacaatgca gcaatacctg cagcgataca
     1861 agcataagga tttgcacctg tagagcctgt taaacgtact gtagaagtag atgcattttg
     1921 ctcgtggtct gcgtgtaata caaaaatacg atccattgct ttagcaatga ttggattaac
     1981 tttgtattct tcacagggtg ttgcaaacat catgtgtaag aagttttcag agtagcttaa
     2041 gtcattgcgt gggtacataa agggttgacc catagaatac ttatagctca ttgctgctat
     2101 cgttggcatt ttagcaatca aacggattgc cgcaatgtga cgatgctctg ggttattgat
     2161 gtctaagttg tcgtggtaaa atgcagacaa tgcacctaca acaccacaca ttactgccat
     2221 tgggtgagca tcacgacgaa aaccacggta gaagaactga agttgttcgt gaaccatagt
     2281 gtgactagaa attaattgtt caaacgcaac tttttgctct gcattgggta attcgccatt
     2341 taataataaa tagcaagttt cgaggtagtc tgatttttct gctaactggt caattgggta
     2401 gccacggtgc aacaaaatgc ctttatcgcc gtcaataaag gtaatcttag attcgcaagc
     2461 agaagtcgcc ataaaaccag ggtcataggt aaagtagccg ttacttaaaa cgtcttttac
     2521 atcaataaca tctgggccta aagtgccgct ataaataggc aggttgatac ttttatcacc
     2581 aacggttaac gtggctgttt tgcccgtcat ggcagtctcc tgtggttttt cagtgcaata
     2641 gcaagcagga cttgacgcat cgtgagtgcg tattaaaatt tcatgtccta attcaaactt
     2701 attagataat taataagtct aatttgagga actcaaactt tttagccaaa gtccaagaat
     2761 ttttaacaga gtttaaggca aaaatgatgc caatgaaata tcactctgat aataagctct
     2821 tagaatttat gaactcatga catcaaaaga gacatgaggc taataacttt atgagtaatt
     2881 gagcttaaca aaaacggcgt tagcttaaag tttgttatga ttttgtcaat aaagcgagag
     2941 gggctaaacg taaacgattg tgtttaaatg ccgttattct gttcgattgc ttaaaaatta
     3001 gactgacaat gaaacaaaaa taaagtaatt aacgaaaaaa gtctgttttg tacaaggctc
     3061 gaactgtatc ttgtttgtaa tctcgcgcta agatgactat aatttcgcgc cgtcnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttggca acgccgccac ttgtcgaata
     3241 aagccagcag tcatctgccc gtcattttga accttcaaaa gagggggaga ctacgcttta
     3301 ttcctccctt tggtttcgct ctattttgag ccttaaatta cgcagggtgc ccgccgtgaa
     3361 aagcaataga cctgtcaact tgtcattagg tatggttctc gaagtgaata tgaagtcgcc
     3421 tgttgcgatg gcttctattc tgcatcgtat ttcgggaatt gtcgtgttct tgttgattcc
     3481 tgtccttctt tatattttgc aaggttcttt ggcttccgaa gagtctttta actacgttaa
     3541 agacaccatt ttaggtggtt ttattggccg ttttgttctt ttcgtggctc ttgcaggttt
     3601 aatttaccat ttctttgctg gtattaaaca cttagtcatg gattttggta ttgctgaaga
     3661 gttaaaaagt ggtcgtgctt ttgcaagtgc cattttagct atttctgcct ttttcatcgc
     3721 cttggccttt tattgggtgg tattgtcatg aaaagtgcaa ccagttttag tcgttctggc
     3781 atgagcgact ggctcgtaca acgtgtcagt gctgttattc tagctgtcta ttttgtggtg
     3841 attgttggtt ggttattaac tgctggccaa gtcacttatg atgcttggca cagttttatg
     3901 acttgcacag ccatgcgtat ttttagcttg ttagctttat tatcgttagc tggccactgt
     3961 tgggtaggtt tatggactgt gaccacagac tatatgacta ctcgtcaaat gggtgcaagt
     4021 gcaaactttt tacgcgtaat tactcaaatc gctatggcaa ttgctatttt tgtctatgtg
     4081 gtttggggtg ctatcatttt gtggggtaat taagtaatgt ctcatagcaa tattcgtacc
     4141 ctcagttttg atgccgttat cgtcggtggt ggcggttctg gtatgcgagc ttccttacaa
     4201 ttagctcgtg ctggtttaag tgttgccgtt ttaaccaaag tgtttccaac ccgttcacac
     4261 actgttgccg ctcaaggtgg tattggtgcg tctttgggta acatgagcga agacaattgg
     4321 cattatcact tctttgatac cgtcaaaggt tctgactatt tgggcgacca agacgcgatt
     4381 gagtttatgt gtcgtgaagc tcccaaaatg gtctatgaat tagaacatat gggtatgcct
     4441 tttgaccgta acgaagatgg cactatttat cagcgtccat tcggtggtca tacttctaac
     4501 tatggcgaaa agccagttca acgtgcttgt gcggctgctg accgtacagg ccatgccttg
     4561 ttgcatactt tgtatcaaca aaatttacgc gctaaaacca aattctttgt cgaatggatc
     4621 gctttagatc taattcgtga tgaagaaggt gatgttttag gtgtcacggc aattgacctc
     4681 gaaacaggtg aagttgcggt tttccaagcc aaatgtactt tatttgccac aggtggtgca
     4741 ggtcgtattt atgctgcatc tacgaatgcc ttcattaaca caggtgatgg tttaggtatg
     4801 gcagcccgtg caggtattcc attagaagat atggaattct ggcaattcca cccaacaggt
     4861 gtggcgggtg caggggtatt gttaaccgaa ggttgtcgtg gtgaaggtgg tttgctccgt
     4921 aacaaaaacg gcgaacgctt catggagcgt tatgcaccaa acctcaaaga cttagctcca
     4981 cgtgacttcg tgtctcgctc tatggaccaa gaaatcaaag agggtcgtgg ttgtggtcct
     5041 aatggtgatt atgttgttct tgacttaact cacttaggcg ttgaaaccat tatgaaacgc
     5101 ttaccgtctg tttatgagat tggtaagaag tttgctaacg ttgattgtac caaagagcca
     5161 attcctgttg tgccaaccat tcactatcaa atgggcggta ttccaaccaa catcaatggt
     5221 caagtcgttg tgcctaaaga cggtaatcca aacactgttg ttaaaggttt atacgcgatt
     5281 ggtgagtgtt cttgcgtcag cgtacacggt gctaaccgtt taggtactaa ctctttgtta
     5341 gacttaatcg tgtttggtaa ggctgctggt gagcatatta ttgatactgt acgtcgtgcg
     5401 ccacatgagt ttaagccatt acctaaaggc gtgttagatg tcactttagc gcgtattaaa
     5461 aaattagatg attcaacttc tggtgaatat gctcaagata ttgctaatga tttgcgtaaa
     5521 atcatgcaag ctcatgcagg tgtattccgt acccaagc
//
LOCUS       DAOQMB010000123         5557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6516_length_5557_cov_16.341694, whole genome shotgun sequence.
ACCESSION   DAOQMB010000123 DAOQMB010000000
VERSION     DAOQMB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6516_length_5557_cov_16.341694"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..872
                     /gene="mlaE"
                     /locus_tag="PLY05_05630"
     CDS             99..872
                     /gene="mlaE"
                     /locus_tag="PLY05_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HNC02819.1"
                     /translation="MIRQLGASTLNRLQAIGAAGVFLWQALVAVPQPKRSFLLLVRQL
                     YAVGVMSLPIILVSGLFIGLVLGLQGYNILVTYGSEQALGTMVALTLVRELGPVVTAL
                     LFAGRAGSALTAEIGLMKATEQLASMEMIGVDPLRRIIAPRFWAGFISMPLLAIIFSA
                     VGVIGGMLVGVDWLGIYAGSFWSNMQNSVEFYDDVLNGIIKSLVFGITVTWIALFQGY
                     DCVPTSEGISVATTRTVVYGSLAVLGLDFILTAVMFGGF"
     gene            872..1393
                     /gene="mlaD"
                     /locus_tag="PLY05_05635"
     CDS             872..1393
                     /gene="mlaD"
                     /locus_tag="PLY05_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HNC02820.1"
                     /translation="MRTRSLELAVGAFIVISVAALFFLALKVSGLSSELNQPSYKVYA
                     HFQNIGGLTVRAKVTMSGVAIGRVSAIELDKKRLNAKVTLEIYNDVNEISTDSVASIL
                     TAGLLGEKYIGIVPGADDTFIKNGDEIEQTQSSLVLEDLIGKFLFNKATEDKAPAKAE
                     TPAAAPATTQDEF"
     gene            1408..>2018
                     /locus_tag="PLY05_05640"
     CDS             1408..>2018
                     /locus_tag="PLY05_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNC02821.1"
                     /translation="MSVIVKRAFALFTMMSMMLFAQLSLAAENPKDFVQRISTDLTTR
                     LTKEKAAYQKDPQLLLKIVQENIEPYVDFNGFAKGVMGQFYRQASDKQRADFTDIFKK
                     SLIKTYANGLGAYENQKFTVKPFVAGDDPKKAQVDMDIQTNSSTTIPVTYQLVLDAQG
                     QWKVRNVLLNGINLGLTFRNQFASTVEANRGNLDKAIANFVPSA"
     assembly_gap    2019..2118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2119..3361
                     /gene="murA"
                     /locus_tag="PLY05_05645"
     CDS             <2119..3361
                     /gene="murA"
                     /locus_tag="PLY05_05645"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005406655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNC02822.1"
                     /translation="GGNRLAGDVRISGAKNSALPLLAGTILASTPVSLDNVPDLHDIK
                     TMIRILQNMGMTIERQADGKVLINANTLDKPVAPYELVKTMRASIFVLGSLVTRFKEA
                     YVSLPGGCAIGSRPVDQHLKALEALGATVTVEKGYVHAKVDGRLRGGRIFFDMVTVGG
                     TENAIMAATLAEGTTIIENAAREPEVVDLANMLIKMGAKIEGAGTDRIIVHGVDELQG
                     CEYSVCPDRIETGSYLAAAVMTGGRIRATHTDPALLDAVLLKFLEMGASVTTGTDWIE
                     VDMQGKRPRAVNFRTAPHPAFPTDMQAQFMAINVIAEGTGTINETIFENRFMHVPELN
                     RMGANIEVDGHTAVVRGVAKLEGAPVMATDLRASMSLVLAAVAAEGDTLIDRIYHIDR
                     GYERVEEKLSLLGANIRRVKA"
     gene            3380..4018
                     /gene="hisG"
                     /locus_tag="PLY05_05650"
     CDS             3380..4018
                     /gene="hisG"
                     /locus_tag="PLY05_05650"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNC02823.1"
                     /translation="MSQLTIALSKGRILKDTLPLLKVAGIEMLEDPEKSRKLIFPTTH
                     PQVNIIIIRATDVPTYVENGAADLGVAGKDVLMEHGASGVYEPLDLDIAKCKLMVAGK
                     VDAPPVNGRLRIATKFVNLTKQYYAQKGEQVEVIKLYGSMELAPLMGLADRIVDVVDT
                     GKTLKENGLEPLELISYISSRLIVNKASFKRKSDLIQPLLQQLSQAVAENKV"
     gene            4031..5329
                     /gene="hisD"
                     /locus_tag="PLY05_05655"
     CDS             4031..5329
                     /gene="hisD"
                     /locus_tag="PLY05_05655"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNC02824.1"
                     /translation="MRRLSTTAPEFEQELNQLLAWESVNDHQLQQTVLDIISRVKQQG
                     DTAVLDYTRRFDQVTAETMAGLSLSAEQLSTAYHNLNEQQRNALASAAERIRYYHGKQ
                     KQDSWQYQEVDGTMLGQQITPLDRVGIYVPGGKASYPSSVLMNAIPAKVAGVPEVIMC
                     VPTPRGEVNNLVLAAAYLAGVDKVFTIGGAQAVAALAYGTATIPKVDKIVGPGNSYVA
                     TAKRLVFGQVGIDMIAGPSEILIYCDGKTNPDWIAMDLFSQAEHDEEAQAILISEDAA
                     YLDAVQQSIDKLLPTLERAVIARASLTGRGALIHVKNQQEALAVINRIAPEHLELSVD
                     NPEALLPYIRHAGAIFMGRYTPEALGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSS
                     LIYCSAQGASHLAEVASVLARGESLTAHARSAEYRILSEE"
     gene            5334..>5557
                     /locus_tag="PLY05_05660"
     CDS             5334..>5557
                     /locus_tag="PLY05_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02825.1"
                     /translation="MDLADKFVQFAESGNQQRLQTKDGRMLQGWIMEITDDALLMSIG
                     TGEKGQDVWLKLVDVEPTSLAYFHSHSQQW"
BASE COUNT         1494 a         1015 c         1306 g         1642 t
ORIGIN      
        1 caacagtttt taaatggtag tcctgatggg ccagtgcgtt ttcactatcc tgcctctagt
       61 tatctacaag aattaggagt atcaaactga tggtcgaaat gattcgacaa cttggtgctt
      121 ccaccttaaa tcgattacaa gctattggtg cagcaggggt gtttttatgg caagcccttg
      181 ttgccgtacc gcagcccaaa cgttcatttt tattattagt acgtcaatta tatgccgttg
      241 gcgtgatgtc cttaccgatt attttggtat caggtttatt catcggttta gtattggggt
      301 tgcagggtta taacatcctt gttacctatg gtagcgaaca agctttaggc acaatggtag
      361 ctttaacttt agtgcgtgaa ttagggcctg tagtcaccgc tttattattt gcagggcgag
      421 caggctcagc attaaccgca gaaattggtt taatgaaagc taccgaacaa ctcgccagca
      481 tggaaatgat aggtgttgat cctttacgcc gtattattgc accacggttt tgggcaggtt
      541 ttattagtat gcctttatta gccatcatct tctcagccgt tggtgtgatt ggtggtatgt
      601 tggtaggtgt tgattggttg ggcatttatg caggttcatt ttggtcgaat atgcaaaatt
      661 cggtcgagtt ttatgatgat gtgcttaatg gcattatcaa aagtttagta tttggtatta
      721 ccgtgacgtg gattgccttg tttcaaggtt atgactgtgt tcctacttca gaaggtattt
      781 ctgttgcaac gacacgtacc gtcgtatatg gctctttagc ggtgttaggc ttagatttta
      841 ttttaaccgc cgttatgttt ggaggttttt aatgcgtact cgctccctag aactagcggt
      901 tggggcattt attgtcatta gtgttgccgc gttatttttc ttggctctta aagtgagtgg
      961 cttaagttct gagttaaatc agccaagtta caaagtttat gcgcattttc aaaatattgg
     1021 tggtttaacc gtccgtgcca aagtcaccat gtctggtgtt gctattggtc gtgtgtcagc
     1081 cattgagtta gataaaaaac gtttaaatgc caaagtgact ctcgaaattt ataatgatgt
     1141 caatgaaatt agtaccgact ctgtggccag tattttaacc gcaggcttat taggcgagaa
     1201 atatattggt attgtgcctg gtgctgatga cacatttata aagaacggtg acgaaatcga
     1261 acagacacaa tcctcattag tgttagaaga tttaataggc aagtttttat ttaacaaagc
     1321 cacggaagac aaagcgccag ccaaagcaga aacacctgcg gctgcacctg caacaactca
     1381 ggatgagttt tgatgaggat tttaaatatg tcagttattg taaaacgcgc gtttgcgcta
     1441 ttcactatga tgagcatgat gttgtttgca caactgagtt tggcggcaga aaacccaaaa
     1501 gattttgtgc aaagaatttc taccgatcta acgactcgtt taaccaaaga aaaagcggct
     1561 tatcaaaaag acccacaatt attgttaaaa attgttcaag aaaatattga accgtatgtt
     1621 gattttaatg ggtttgccaa aggggtgatg gggcaatttt atcgtcaagc gagcgataaa
     1681 caacgtgctg attttaccga tattttcaaa aaaagcttaa ttaaaaccta cgctaatggc
     1741 ttaggtgctt acgaaaacca aaagtttacg gtaaaaccgt ttgtcgcggg tgatgatccc
     1801 aaaaaagcgc aagtagatat ggatattcaa accaatagca gtactaccat tccagtgact
     1861 tatcaattgg tgttggatgc tcaaggccaa tggaaagtgc gtaatgtatt attaaatggc
     1921 attaacttgg gtttaacatt ccgtaatcag tttgcttcga cggttgaagc aaatcgtggt
     1981 aatttagaca aagcgattgc taactttgtg ccttccgcnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnntg gcggtaaccg cttagcaggt gatgtgcgta tttcgggcgc
     2161 aaaaaattcc gctttgccat tgttggcagg aacaattttg gcctcgacac ctgttagctt
     2221 agataacgtg cccgatttgc atgacattaa aaccatgatt cgcattttgc aaaacatggg
     2281 tatgaccatc gaacgtcagg ctgatggtaa agtgttgatt aatgccaata ccttagacaa
     2341 acctgttgct ccgtatgagt tagtaaaaac catgcgtgcc tcaatttttg tcttgggttc
     2401 tttggtaacg cgctttaaag aagcctatgt ctctttacca ggtggctgtg ctattggttc
     2461 gcgtcctgtt gatcagcatc tcaaggcatt agaagctcta ggcgcaacag taaccgtcga
     2521 aaaaggttat gttcatgcca aagttgatgg ccgtttgcgt ggtggtcgta tcttctttga
     2581 tatggtgacg gtgggtggca cagaaaacgc aattatggcg gccactttag ccgaaggcac
     2641 aaccatcatt gaaaatgccg cacgtgaacc agaagtggtt gatttggcta atatgctcat
     2701 caaaatgggc gcaaaaattg aaggtgcagg tacagatcgc attattgtgc atggtgttga
     2761 tgaattacaa gggtgtgaat attctgtttg tcctgatcgg attgaaacag gctcatactt
     2821 agccgctgcg gtaatgacag gtggtcgtat tcgtgccacg cataccgatc ctgctttatt
     2881 ggatgctgtg ttgttaaagt tccttgaaat gggggcttct gtgaccacag gtacagactg
     2941 gattgaagtg gatatgcagg gcaaacggcc acgtgctgtt aacttccgca cagcaccaca
     3001 ccctgctttt ccaactgata tgcaagctca gtttatggca attaacgtca ttgccgaagg
     3061 cacaggcacg attaacgaaa ccatttttga aaaccgcttt atgcacgtcc ctgagttaaa
     3121 tcgtatgggg gctaatattg aggttgatgg tcacacggcg gtagtgagag gagtcgcaaa
     3181 attagaaggt gcgccagtca tggcaaccga tttacgtgcg tccatgtcct tagtcttggc
     3241 agcagtagcc gccgaaggtg ataccttgat tgaccgtatt tatcatattg accgtggtta
     3301 tgagcgtgta gaagagaagt tatcgttatt aggtgctaat atacgcagag tgaaagctta
     3361 aaccaagaga atcatttgaa tgtcgcagtt aactattgct ttatctaaag gacgtatttt
     3421 aaaagatact ttgccactgt taaaagtggc aggtattgaa atgttggaag accccgaaaa
     3481 aagtcgtaaa ttgatttttc cgaccacaca tccacaagtg aatattatta ttattcgtgc
     3541 gactgatgtg ccaacttatg ttgaaaatgg cgcagcagat ttgggtgtgg caggtaaaga
     3601 cgtgctcatg gagcatggtg caagtggggt atatgagccg ctagatttag atattgccaa
     3661 gtgtaagtta atggtcgcag gcaaagtaga tgcgccacct gtgaatggtc gtttgcgtat
     3721 tgccactaaa tttgtcaatt tgactaagca atactatgcg caaaaaggcg aacaagttga
     3781 agtcattaaa ctctatggtt ctatggaatt agccccttta atgggcttag ctgatcgtat
     3841 tgttgatgtg gtcgatactg gcaaaactct aaaagaaaat ggtttagagc cactggaact
     3901 catttcctat atttcttcac gattgattgt taataaagcg agttttaaac gtaaatccga
     3961 tttaattcag cctttattgc aacaactctc gcaggcagtc gccgaaaata aggtgtaatt
     4021 tatggcatcc attcgtcgtt taagcacaac tgctccagag tttgagcagg aattaaatca
     4081 gctattagcg tgggaaagcg ttaacgatca tcagttgcaa caaacggtgt tagatattat
     4141 tagccgtgtt aaacaacaag gtgacaccgc tgttttagat tatacccgtc gttttgatca
     4201 agtaacggct gagacaatgg ctggcttgag tttgtcggca gagcaattgt ctacggctta
     4261 tcacaactta aatgagcaac aacgtaatgc gttggccagt gcggccgagc gtattcgtta
     4321 ttatcatggc aaacaaaaac aagattcatg gcaatatcaa gaagttgatg gcacgatgct
     4381 cggtcaacaa attacgccat tagatcgcgt gggtatttat gtacctggtg gtaaagccag
     4441 ttatccttca tcggtgttaa tgaatgctat tcctgccaaa gtggcaggtg tacctgaagt
     4501 gattatgtgt gtgccaacac cacgcggcga agtcaataat ttggtattag cagcggctta
     4561 tttagcaggt gttgataaag tatttaccat tggtggggcg caggctgttg cggctttagc
     4621 ctatggcaca gcaaccattc ctaaggttga taaaattgtt gggccaggta atagttatgt
     4681 cgcaaccgct aaacgtttag tgtttggcca agtcggcatt gatatgattg caggcccttc
     4741 cgagatttta atttattgtg atggcaaaac taatccagat tggattgcca tggatttatt
     4801 ttctcaagct gaacacgacg aagaagcaca agccatttta atcagtgaag atgcagcgta
     4861 tttagacgcg gtacaacaat ctattgataa attgttgcct actttagagc gtgcagtgat
     4921 tgctcgtgct tcgttaacgg ggcgtggtgc attaattcat gttaaaaatc aacaggaagc
     4981 cttagcggtg attaaccgta ttgcgcctga gcacttagag ttatctgttg ataatccaga
     5041 agctttattg ccgtatattc gtcatgcggg cgcaattttt atggggcgtt atacgcctga
     5101 agccttaggt gattattgtg cagggccaaa tcacgtcttg ccaacatcgg gtacagcccg
     5161 tttttcttcc cctttaggcg tttatgattt tcaaaagcgt tctagtttga tttattgctc
     5221 cgcgcaaggt gcaagtcatt tagcagaagt ggcttcagta ttggcgcgag gtgaaagttt
     5281 aacggctcat gcacgttcag cagagtatcg tattctttct gaggaataag cagatggact
     5341 tagccgataa atttgtccaa tttgccgaat caggaaatca acaacgtttg caaaccaaag
     5401 acggtcgcat gttgcagggt tggattatgg aaattactga cgatgccttg ttaatgagca
     5461 tcgggacagg cgagaaaggc caagatgttt ggctaaagct agtcgatgtt gaaccgacta
     5521 gtcttgctta ttttcatagt cacagccaac agtggca
//
LOCUS       DAOQMB010000124         5532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6571_length_5532_cov_17.123425, whole genome shotgun sequence.
ACCESSION   DAOQMB010000124 DAOQMB010000000
VERSION     DAOQMB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6571_length_5532_cov_17.123425"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1025)
                     /locus_tag="PLY05_05665"
     CDS             complement(138..1025)
                     /locus_tag="PLY05_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC02826.1"
                     /translation="MAELTIKAFFEPETETYSYVVSAGTNSAAAIIDPVLDFDAKSGR
                     TFTKFADQLIAYIKQEKLTVEWILETHAHADHLSAAFYLKQQVGGKIGIGEKITQVQK
                     VFKQLFNLEPDFATDGQQFDHLFTDNEQFSIGQLSAKVILVAGHTPADVAYQIEDAVF
                     IGDSLFMPDVGTARCDFPQGNARTLYQSIQKILSLPDETRLFMCHDYPPIERKEYLYI
                     STVKEQKEKNIHIRQGISEEQFVEMRQQRDASLAMPRLIIPAIQINIRAGAFPPADTN
                     GVVYLKLPLNLLGKKSNKP"
     gene            1136..1438
                     /locus_tag="PLY05_05670"
     CDS             1136..1438
                     /locus_tag="PLY05_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC02827.1"
                     /translation="MSDLAQMQAAAGKASSLLKALSHPDRLILLCQLSQGEYCVSELE
                     EMLGIHQPSLSQQLGVLRQESLVETRRDGKQMYYRIASEDALAILQVLYNRFCPPS"
     gene            1454..1882
                     /locus_tag="PLY05_05675"
     CDS             1454..1882
                     /locus_tag="PLY05_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HNC02828.1"
                     /translation="MLIDWPAFTPYSALISGVLIGLASAMLIMCVGRVAGISGIVGGL
                     FSGQHHEAWRWYFLIGLLLAPWLYQLFAVLPPMQISNNIPLLIVSGLLVGIGTRYGSG
                     CTSGHGVCGLSYLSLRSLVATLSFMAMGFLTVYVIKHLVA"
     gene            1900..2310
                     /locus_tag="PLY05_05680"
     CDS             1900..2310
                     /locus_tag="PLY05_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNC02829.1"
                     /translation="MFKIIALLSGIIFGFGLIVSNMINPAKVIAFLDVAGMWDPSLAL
                     VMAGAIVVAMPVFQLAKHRQASLCGLPMQLPISKVIDKRLVIGSLLFGVGWGIAGFCP
                     APALVSAATGQAEAIIFSLAMLTGFYLFAWLEKR"
     gene            2319..>3979
                     /gene="sulP"
                     /locus_tag="PLY05_05685"
     CDS             2319..>3979
                     /gene="sulP"
                     /locus_tag="PLY05_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HNC02830.1"
                     /translation="MPAWLNQVRHTWQDDLLAGMIVGIVVIPQSLGYALLAGLPPVYG
                     LYAAIIPVLLYAYIGASQTQAVGPVAITSIMTAQALLPFAQSQSLTYIALAACLALLV
                     GLVLLGAAWLKLGWIMQFMSRGVMAGFITGAALLIMTGQCKYILGLNVQGDTVWQLLA
                     GFYQQQALINQQAVILGLVAVFLLIANRHYSQILLVKLGFSLYLANIISKLIPIMVIA
                     GASLLVWQQSLSLAIVGDIPIGLPQFVWPALPIVEQWRALISSALLIALVAFISSASV
                     AKSVALQRQEQFDANKELLGLGCANIGAAFFQGLPITGGFSRTAVNLEAGAKTPLAGV
                     VSALVIAVVLVLLGTWFYYLPLAVLGAGVIVAVSQLLDVQTVKMAWQHDKSEAYAYLA
                     TLMGVLLAGLQVGLLLGLFVSLAALIWRSHQPHVAVVGQVGTTEHFRNVQRHQVTTWP
                     NLLLIRIDENLFFGNSESIANRIWLEINQQPKLTDVVLIFSAVNHIDLSSQLMLDNLQ
                     QQLAQRQINIHFAEIKGFVMDTLVNTPLLKNWQGKIFDTTEQAAN"
     assembly_gap    3980..4079
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4083..4373
                     /locus_tag="PLY05_05690"
     CDS             4083..4373
                     /locus_tag="PLY05_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HNC02831.1"
                     /translation="MQEVASRGFKSVINNRPDYEEGAQQPTAAQVQAAAEAQGLSYVH
                     QPVISGQITQENVEQFAKYINELPKPVLAFCRTGNRCNNLYQLAKQMDLLDD"
     gene            complement(4418..>5532)
                     /locus_tag="PLY05_05695"
     CDS             complement(4418..>5532)
                     /locus_tag="PLY05_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC02832.1"
                     /translation="IMNPTTAVLEQRLAQIEGGIGGLALASGMAAITYAIQAITEVGD
                     NIVSTSQLYGGTYNLFAHTLPRQGIEVRFASHDDYEAFDRLIDSKTKAVFCESIGNPA
                     GNVVDLQRLAEIAHKHGVPLIVDNTVATPVLCRPFEHGADIVVHSLTKYIGGHGTTIG
                     GMIIDSGKFDWVANKDRFPMLNEPDPSYHGVVYTAALGAAAYIGRCRVVPLRNTGAAL
                     APHSAFLLLQGLETLALRMERHCSNALAVARYLANHPQVSWVNYAALDNSPYNTTCQK
                     ISNGYASGILSFGIKGGKEAGAKFIDGLKMILRLVNIGDAKSLACHPASTTHRQLNAD
                     ELKRAGVSEDMVRLSIGIEHIDDIIADIEQALTQAV"
BASE COUNT         1520 a          981 c         1180 g         1751 t
ORIGIN      
        1 atacgagtgt atcgttgata gttggtgaca gcatttgcga gatgatgaca agttttttga
       61 tggatggcga aaatttaact aattgatttt aaataatttt tttagagtgt ttgctctagt
      121 aaattagagt ttttactcta tggtttgttg ctttttttac ctagtaaatt taatggtaat
      181 tttaaataaa ccacaccatt agtgtcggct ggtggaaaag ctcctgcacg aatattaatt
      241 tgaatggcag gaataattaa gcgaggcata gctaaactgg catcgcgttg ttggcgcatt
      301 tcaacaaatt gctcttcgct aattccttga cggatatgaa tgtttttttc tttttgttct
      361 ttaaccgtac ttatataaag gtattcttta cgttcgattg gcggataatc atggcacata
      421 aacaaccgtg tttcgtctgg caaagacaaa attttttgaa tggattgata taacgtacga
      481 gcatttcctt ggggaaagtc gcaacgagct gtgcccacat ctggcataaa taaactatca
      541 ccaataaaaa ccgcatcttc aatttgatag gcaacatcag caggggtatg acctgcaacc
      601 aaaatgacct tggcactgag ttggccaatg ctgaattgtt cgttgtctgt aaacaaatga
      661 tcaaattgtt ggccatctgt tgcaaaatct ggctctaaat taaagagttg tttaaaaact
      721 ttttgtactt gagttatttt ttcacctata ccaattttgc cacctacttg ttgttttaaa
      781 taaaatgcgg cactaagatg atcggcatga gcatgggttt ctaaaatcca ttcaacagtc
      841 aatttttctt gtttaatata tgctattaat tggtcggcaa atttggtgaa ggtacggcca
      901 gatttggcat caaagtctaa tacagggtca ataatggccg ccgcagaatt tgttccagca
      961 cttacgacat aactataagt ttcggtttct ggctcaaaaa aggctttaat tgttaactca
     1021 gccattgttt ttgtcctcaa attagattta atacgagcaa agaattaaag tcgctatatt
     1081 aattaataaa atgttttttt gtatagtgtt gtgtataatt taatgagtat gtcatatgtc
     1141 agatttagca caaatgcaag cggctgctgg aaaagcatct agtttattaa aagctttatc
     1201 tcatcctgat cgattgatcc tattatgtca attatcacaa ggagaatatt gcgtcagtga
     1261 attagaagaa atgttgggga ttcatcagcc aagtttgtca cagcaattag gtgtgttgcg
     1321 tcaagagtca ctggttgaaa cccgacgtga tggcaaacaa atgtactatc ggattgcgag
     1381 tgaagatgcc ttagccattt tacaagtgtt atataaccgt ttttgcccac ccagttaaat
     1441 ttgagagtct taaatgttga tagattggcc tgcttttacg ccctacagtg ctttgattag
     1501 tggtgtatta attggtttag cctcagcaat gctgattatg tgtgtagggc gagtagcagg
     1561 cattagtggt attgttggtg gattattttc tggtcagcat catgaagctt ggcgttggta
     1621 ttttttaata ggtttattgc ttgccccgtg gttatatcaa ctgtttgccg tgttaccgcc
     1681 aatgcaaatt agcaataata tccctttgtt aatagtgtca ggtttattag ttggcatcgg
     1741 tacacgttat ggcagtggtt gcaccagtgg tcatggggtg tgtggtttat cttatttgtc
     1801 tttgcgttct ttagtggcga ccttaagttt tatggcaatg ggatttttga cagtgtatgt
     1861 cattaaacat cttgttgctt aaaggaggct gtcattatca tgtttaaaat cattgcgtta
     1921 ttatcgggta ttatttttgg ttttggatta atcgtcagta atatgattaa tcctgccaaa
     1981 gtgattgctt ttttagatgt ggctggcatg tgggatccat ctttagcctt agtgatggca
     2041 ggtgcaatag tagtcgcaat gcctgttttt caacttgcta aacataggca agccagtttg
     2101 tgtggtttgc caatgcaatt acctatcagc aaagttattg ataaacgtct agttattggc
     2161 agtttattat ttggcgtcgg ttggggaata gcaggttttt gtcctgcgcc tgcattagtg
     2221 agtgccgcaa caggccaagc cgaagcgatt atttttagct tagcaatgtt aacaggcttt
     2281 tacttgtttg catggttaga aaaacgatga aaacatttat gccagcttgg ctaaatcagg
     2341 tgcgccacac atggcaagat gatttgttgg caggcatgat tgttggtatt gtggttattc
     2401 cccaaagttt aggttatgcc ctgttagcag gtttaccacc tgtctatggt ttgtatgccg
     2461 ctattattcc tgttttgtta tatgcctata ttggtgctag tcagactcaa gcggttggcc
     2521 cagtagccat tacctcaatt atgaccgcac aagctttatt gccttttgcc caaagtcaaa
     2581 gcttaactta tattgcatta gcggcttgtt tggccttatt ggtgggttta gtgttgttag
     2641 gcgcagcatg gctaaaactc ggttggatta tgcaatttat gagtcgtggg gtgatggctg
     2701 gttttattac aggcgcggct ttattaatta tgacagggca gtgcaaatat attttagggc
     2761 taaacgtaca aggagatact gtttggcagc tcttagcggg tttttatcag caacaagcat
     2821 tgattaatca acaggccgtc atcttaggtt tggtggcggt atttttattg atagctaacc
     2881 gtcattatag ccaaatactg ttggtaaaat taggtttttc gctttatctt gccaatatca
     2941 ttagcaaatt gataccgatt atggtgattg caggtgcgag tctgttggtc tggcaacagt
     3001 cattaagctt ggcaatagtt ggtgatattc ctattggttt gccgcaattt gtctggccag
     3061 ctctaccaat cgtcgaacag tggcgcgcgc tgatcagctc agccttgtta atcgctttag
     3121 ttgcgtttat tagcagtgcc tctgtcgcaa agtcggttgc cttgcaacgt caagaacagt
     3181 ttgatgctaa taaagaatta ttaggtttgg gatgtgccaa tattggtgct gctttttttc
     3241 aaggcttgcc aattacaggt ggtttttcgc gtactgcggt caacttggag gcaggggcaa
     3301 aaacaccttt agctggtgtg gtttcggctt tggtgattgc cgttgttctc gtgttattag
     3361 gcacatggtt ttattattta cctttagcgg tattaggtgc aggggtaatt gttgctgtta
     3421 gtcagttatt agatgtacaa accgtaaaaa tggcttggca acacgataaa agtgaagcct
     3481 atgcctactt agcaacttta atgggcgtat tgttagcagg tttacaggta ggattattat
     3541 taggtttgtt tgtgtcttta gcggcattga tttggcgtag tcatcaacct catgttgcgg
     3601 ttgttggtca agttggtacg acagagcatt ttagaaatgt gcaacgtcat caagtgacaa
     3661 catggccaaa tttgttgtta atcagaattg atgaaaattt attttttggc aatagtgaaa
     3721 gtattgccaa tcgaatttgg ctagaaataa atcaacaacc taagctcaca gatgtggtat
     3781 taatttttag tgcagtcaat catattgatt tatcatcaca gttaatgcta gacaatctac
     3841 aacagcaatt agctcagcga cagattaata ttcattttgc tgaaattaaa ggctttgtga
     3901 tggatacttt agtcaatacg cccttgttaa aaaattggca aggtaaaatt tttgacacaa
     3961 cagagcaggc agcaaatagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     4081 aaatgcaaga agttgcaagc cgtggcttta agtcggtgat taataaccgc ccagattatg
     4141 aagaaggcgc acaacaacca actgccgcac aagtacaagc tgctgccgaa gctcaaggtt
     4201 taagttatgt acatcagcct gtgattagtg gccaaattac ccaagaaaat gttgagcaat
     4261 ttgccaaata tatcaatgaa ttacctaaac ctgttttagc gttttgccgc acaggtaatc
     4321 gctgcaacaa tttatatcaa ttagccaaac aaatggactt attggatgat tagtttaaaa
     4381 attgggactt aggttaattc ctaagtcctt caatccttta aacagcctga gttaatgcct
     4441 gttcaatatc ggcaataata tcatcaatat gctcaatccc aattgataag cgcaccatat
     4501 cctcagacac gcctgcgcgt tttaactcat cagcgtttaa ctgacgatgg gtagtactag
     4561 caggatggca agctaaagat ttagcatcgc ctatgttaac caaacgtaaa atcattttta
     4621 ggccatcaat gaactttgcc cctgcttctt tgccaccctt aatgccaaag cttaaaatgc
     4681 cagacgcata accattgcta attttttggc aggttgtgtt atatgggctg ttatctaaag
     4741 cggcatagtt aacccaagaa acttgtgggt gatttgctaa ataacgcgca acagctaaag
     4801 cattactaca atgacgctcc atgcgtaagg caagcgtttc taagccttgt aataataaaa
     4861 atgcactatg tggtgcaagt gccgcacctg tattgcgtaa tggcacaaca cgacaacgac
     4921 caatataagc agctgcacct aaagcagcgg tataaaccac accatgataa gacggatcag
     4981 gctcgtttaa catgggaaag cggtctttgt tggccaccca atcaaactta ccgctatcaa
     5041 taatcatgcc accaatggtt gtaccatgac caccaatata tttggttaag gagtgaacga
     5101 caatatcggc accatgttca aaggggcggc ataagacagg cgtagcgacg gtattatcca
     5161 caattaatgg cacaccatgt ttgtgagcaa tctccgctaa gcgttgtaag tcaacaacat
     5221 tgcccgcagg attaccaata gactcacaaa aaacggcttt agttttacta tctatcaaac
     5281 ggtcaaaagc ctcgtagtca tcgtgagagg caaaacgaac ttcaatgcct tggcgtggca
     5341 aagtatgggc aaataaattg tatgtaccac cgtataattg agaggtactg acaatattgt
     5401 caccaacttc ggtaattgct tgaatagcat aggtaatagc cgccatgcct gatgctaacg
     5461 ccaaaccacc aataccccct tcaatttgag ctaaacgttg ttctaaaaca gcggtagtcg
     5521 gattcataat cc
//
LOCUS       DAOQMB010000125         5514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6610_length_5514_cov_7.427551, whole genome shotgun sequence.
ACCESSION   DAOQMB010000125 DAOQMB010000000
VERSION     DAOQMB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5514
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6610_length_5514_cov_7.427551"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLY05_05700"
     CDS             complement(<1..760)
                     /locus_tag="PLY05_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HNC02833.1"
                     /translation="MTFGAITNFVPDENLRNIASKFNPEPSKNLPSIMFGGRSDLKFN
                     NKFLEDYPNSPQKRLEQIQRKHPEVLVLGTNEEYELKSSTFDSLDYVFLEKEPKNSYN
                     YWKLLGLYNGERTYRWIEKKYMEARYPENNNLEKFKVFVPKANGSGMMGEVLSTPVIG
                     TPSMSATPTFISIGAFDTEIEAKNLMNYIKSKFARALLGILKITQDNPPLKWTYIPLQ
                     DFTPNSDIDWSKSIAQIDQQLYGKYGLNQEEIDFI"
     gene            complement(723..4313)
                     /locus_tag="PLY05_05705"
     CDS             complement(723..4313)
                     /locus_tag="PLY05_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HNC02834.1"
                     /translation="MTNNNTLIKTTKLIYPQIYAYITPNYPKNEGWIKIGYTEHQDVD
                     IRIKQQTQTASIDYVTLWARPAQKLHNPNVLFKDKELHAYLRKYKKVEQRPSTEWFFY
                     NGTPEKALADFNDFRADKRSQSTENLDYQLRAEQKAAIEQTLHYAKHNPQGEFLWNAK
                     PRFGKTLTTYDFARQLDAHKVLIVTNRPAIANSWFDDFEKFIAWQTDFAFVSTSDSLK
                     DRPVMTRDEFLKQTASGKNRMLAFISLQDLKGAISFGGSFNKLKWVKDLAWDLLVIDE
                     SHEGVDTFKTDIAFDNITRNFTLHLSGTPFKAVASGKFSQNEIYNWTYADEQQAKANW
                     NNVEENNPYELLPRLNLFSYQMSQMITDTVNQGAQINGQNIDYAFDLNEFFETNDKGN
                     FVYEADVQKWLNALSHNEKYPFSTKELRNELKHTFWLLNRVASAKALQKLLQNHPVFE
                     NYEVILAAGDGRTNEDDQTINQTSLNRVKASIAKHDKTITLSVGQLTTGVTIPQWTAV
                     LMLSNLTSPSQYMQAAFRAQNPWAYAVDGQMRQKENAYVFDFAPERTLIIYDEFANNL
                     SSKTSNGGGTSDDRKDNIKQLLNFFPVIAEDSQGKMVELDVNQVLTIPKAIKAQEVVK
                     RGFMCNLLFNNVSGIFASPEVRQILEQLNPVDVGKTTPRHTEELIDTQGVKIDDDGDV
                     VVAQEIVIAQTNARFGEKVYSKITASVENIASTTSQNLSNIVANAFKENTQADVVALA
                     KEQGLSAKAAEQVVQQSANIIAKEIETVQKQADIKRKEAEVEYKKVVAEAQHDIAKVQ
                     QAKAVYEATQKHIEDNFKKDLVATVEAKSKELVQQSTQTILEKAEDKKKNTVEDDVRA
                     RLRGFARTIPSFLMAYGTPSTTLANFDSTIKASVFKEVTGISLDDFTILRDTHHFFDE
                     TVFNESVQEFLNKRRELGNYFDESQQEDIFDYIPPQQTNQIFTPKKVVKLMIDKLEAE
                     DPNLFTDPHKTFADLYVKSGLYLTEIVKRLYVGLADKMPDPDTRLKHILEQQVYGFAP
                     SEIIFNIARNFVFGFDEKVAKNINDSHIVCLDTTPYAKGQGDFEASCKQFFGAKQMKF
                     DVVVGNPPYQEISLVNNKQDAVYNYFYDLAEKISPKYCLISPARFLFNAGLTSKVWNQ
                     KMLKDEHLKVVYFNQDSSQVFPNTDIKGGGSGIISRYKNDFWGNYKLCPR"
     gene            complement(4316..5299)
                     /locus_tag="PLY05_05710"
     CDS             complement(4316..5299)
                     /locus_tag="PLY05_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011960378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNC02835.1"
                     /translation="MLTSTKATYNTSFEFPTVQCSNDSAQQREHHFEHDVSDIFKNTL
                     REQRSAILDILLIDRTASTVKTTKNIIWANEHYIDYDKDKYAATAHIKPELITGKMCQ
                     LIMPRALKSREHQKERTKSKAEVFTPTWIIKKQNDTLEQDYLNDDLETYVRRKWLEIT
                     CGEAPYMATRYDMATGEAIPLAERVGFVDRKLKRINANIDDKAEWQRLVEEAYKASYG
                     FEWNGDSLLLARENLLYTYQDFYVAKWQQQPTNGLLKEIAEIISYNVFQMDGLKYIIP
                     LSEKKEKITQISFSFSDDEPTQEQWLIQVGKRVKIMNWDKKKMELFDKGVK"
BASE COUNT         1525 a         1037 c         1049 g         1903 t
ORIGIN      
        1 ctataaaatc aatttcttct tggtttaggc catatttgcc ataaagctgt tggtcaattt
       61 gtgcaataga tttgctccag tctatgtcgg agttgggcgt aaagtcttgt aggggaatat
      121 atgtccactt taagggtgga ttatcttgag tgatttttaa tattccaagt aaggctcttg
      181 caaatttcga ctttatgtaa ttcatcaaat tctttgcttc aatctctgta tcaaatgcac
      241 caatagaaat aaatgttgga gtagctgaca tagaaggcgt tccaataaca ggtgtactta
      301 atacttcgcc catcatgccg ctaccatttg ccttaggtac aaaaacttta aacttttcta
      361 aattgttatt ctcaggataa cgagcctcca tatacttctt ttcaatccaa cgatatgttc
      421 tttctccatt atacaatccc aaaagcttcc aatagttata tgagttttta ggttcttttt
      481 ctaagaaaac atagtctaaa gagtcaaaag tggaagattt tagttcatac tcttcatttg
      541 tccctaatac taatacttcg ggatgtttac gctgaatttg ttctagacgt ttttgagggg
      601 agtttggata atcttcaaga aatttattgt taaactttaa atctgaacga ccaccaaaca
      661 ttattgatgg caagttttta gatggttctg ggttaaattt tgatgcaata ttcctcaagt
      721 tttcatctgg gacaaagttt gtaattgccc caaaagtcat ttttatatct cgatataata
      781 ccgctacccc cccccttaat atctgtgtta ggaaatactt gtgaggagtc ctgattgaaa
      841 taaactactt tcaaatgctc atctttaagc attttttgat tccaaacttt tgatgttaaa
      901 ccagcattaa acaaaaaacg tgcaggtgag attaaacaat atttagggct gattttttcg
      961 gccaaatcat aaaaatagtt atagacagca tcttgtttat tattgactaa agatatttcc
     1021 tgatacggtg gattacccac cacaacatca aatttcattt gtttagctcc aaaaaactgc
     1081 ttacagcttg cctcaaaatc gccttgtcct ttggcgtagg gtgttgtatc caaacacaca
     1141 atatgcgaat cgttgatgtt tttagcgact ttttcatcaa aaccaaacac aaaattacgc
     1201 gcaatattaa aaataatctc actgggcgca aagccatata cttgctgctc caaaatatgt
     1261 tttaaccgtg tatcagggtc gggcatttta tccgccaatc ccacatacaa gcgtttaaca
     1321 atttcggtca aatataaacc cgatttaaca tacaaatcgg caaaagtttt gtgagggtct
     1381 gtaaaaagat tggggtcttc agcctccagt ttatcaatca ttagtttaac cacttttttg
     1441 ggggtaaaaa tttgattggt ttgttggggc ggaatgtagt caaaaatatc ttcttgctgt
     1501 gactcgtcaa aataattgcc aagctcacgg cgtttgttta aaaactcttg caccgactca
     1561 ttaaacaccg tttcatcaaa aaagtggtgc gtatcacgca aaattgtaaa gtcatctagg
     1621 ctaatgcctg tcacttcttt aaacacgctt gctttaatgg tgctatcaaa attagcaagg
     1681 gtagtacttg gcgtaccata agccatcaaa aatgagggaa tcgtgcgggc aaaaccacgt
     1741 aaccgcgccc gaacatcgtc ttcaacggtg tttttctttt tatcctcggc tttttctaaa
     1801 atggtttgtg tggactgttg caccaactct ttgcttttag cttcaaccgt ggctacaagg
     1861 tcttttttaa agttgtcttc aatgtgtttt tgtgtggctt catatactgc ttttgcttgt
     1921 tgtactttag caatatcgtg ctgcgcttcg gcaaccactt ttttatattc aacttcggct
     1981 tctttgcgtt taatatcggc ttgcttttgc acggtctcaa tttctttggc aatgatattt
     2041 gccgattgtt gcaccacttg ttcggcagct tttgcgctta aaccttgttc tttggctaaa
     2101 gccacaacat ctgcttgggt gttttcctta aaggcattcg ccactatgtt ggataagttt
     2161 tggctagttg tactcgctat attttctaca cttgccgtta ttttgctata gactttttca
     2221 ccaaaacgcg cgttagtttg agcaatcact atctcttgag caacgaccac atcaccgtca
     2281 tcatcaattt ttacgccttg tgtatcaatc agttcttcgg tgtggcgcgg tgtagttttg
     2341 cctacatcaa cagggttaag ttgctccaaa atttgccgta cttcgggtga ggcaaaaatg
     2401 cctgatacat tattaaaaag taagttgcac ataaagccac gtttaaccac ttcttgggct
     2461 ttaatggctt tgggaatagt tagcacttga ttaacgtcaa gctcaaccat tttgccctga
     2521 ctatcttcgg caatgacagg aaaaaagttt aacaactgtt taatgttgtc tttacggtca
     2581 tcacttgtgc cgccgccatt actggttttg cttgataaat tattggcaaa ttcatcataa
     2641 ataatgagtg tgcgctcagg cgcaaagtca aatacatagg cattttcttt ttggcgcatt
     2701 tgcccatcaa ccgcataagc ccacggattt tgcgctctaa aagccgcttg catatactgg
     2761 cttggtgatg ttaggttgga taacatcagc accgctgtcc attgtggaat agttacacct
     2821 gttgttaatt gaccaacaga cagcgtaata gttttgtcat gcttggcaat ggatgcttta
     2881 acacggttaa gcgatgtttg attgatggtt tggtcatctt cattagtgcg gccatcacca
     2941 gcagcaagga tgacttcgta attttcaaaa actgggtgat tttgcaataa tttttgtaag
     3001 gctttggcac tggcgacacg gtttaatagc caaaaagtat gctttaactc attgcgaagt
     3061 tctttggttg aaaagggata tttttcgttg tgcgataggg cgttaagcca tttttgcaca
     3121 tcagcttcat aaacaaagtt gcctttatca ttggtttcaa aaaattcatt gaggtcaaag
     3181 gcatagtcta tattttggcc attaatttgc gcaccttgat taactgtatc agtaatcatt
     3241 tggctcattt ggtaactaaa caaatttaag cgcggcaata gctcataggg attattttct
     3301 tctacgttgt tccaattggc tttggcttgt tgttcgtctg catatgtcca attataaatt
     3361 tcgttttggc taaacttgcc cgatgcaacg gctttaaacg gtgttcccga taaatgtaag
     3421 gtaaaattgc gtgtgatgtt gtcaaaggca atatcggttt taaaggtatc aacgccttca
     3481 tgagattcat ctattaccag taaatcccat gctaagtctt tgacccattt taatttgtta
     3541 aaactacccc caaacgaaat tgctcctttt aagtcttgca aagaaataaa agctagcatt
     3601 cgattttttc cgctagcggt ttgtttcaaa aattcatcac gcgtcatcac agggcggtct
     3661 ttgagtgaat cgctagtcga aacaaaggca aaatcggttt gccatgctat aaatttttca
     3721 aagtcatcaa accatgagtt ggcaatagca gggcggttgg tgacaattaa cactttgtga
     3781 gcgtctaatt gacgtgcaaa atcataggtt gttaaggttt tgccaaatcg tggcttggca
     3841 ttccacaaaa attcgccttg tggattgtgt ttggcatagt gtagcgtttg ctcaatagcg
     3901 gctttttgct cggcacgtaa ttgatagtct aaattttctg tactttgact gcgtttgtcg
     3961 gctctaaagt cgttaaaatc agcgagtgcc ttttcgggtg tgccattata aaaaaaccat
     4021 tcggtacttg gccgttgttc aacttttttg tacttacgca aataagcatg cagctctttg
     4081 tctttaaaca acacattagg attgtgaagt ttttgagcag gtcttgccca taatgtcaca
     4141 tagtcaatac tggcggtttg cgtctgttgc ttaattcgaa tatcaacatc ttggtgttcg
     4201 gtataaccaa tttttatcca accctcgttt ttagggtaat tgggggtaat ataggcataa
     4261 atttgagggt aaatgagttt agttgtctta atgagggtat tgttgttagt catagctatt
     4321 ttactccttt atcaaaaagc tccattttct ttttatccca attcatgatt tttacacgct
     4381 tgcccacttg aataagccat tgttcttggg taggttcatc atcagaaaat gaaaatgaaa
     4441 tttgggtgat tttttctttt ttttcagaga gtggaataat gtattttaat ccatccattt
     4501 gaaaaacgtt gtaactgata atttcagcaa tttcttttaa taagccattt gtgggttgtt
     4561 gttgccactt agcaacataa aaatcttggt aggtgtaaag taagttttcg cgagccagta
     4621 gcaacgagtc gccattccat tcaaaaccat aacttgcttt gtaggcttct tcgaccaagc
     4681 gttgccattc agctttgtca tcaatattgg cattgatacg ttttagtttt ctgtccacaa
     4741 aaccaacacg ctcggcaaga ggtatggctt cgcctgtcgc catatcataa cgtgtggcca
     4801 tatacggagc ttcaccacaa gtaatttcta accactttcg tctaacatac gtttctaaat
     4861 catcattaag ataatcttgt tcgagggtat cattttgttt tttgataatc catgttggcg
     4921 taaaaacttc tgctttggat ttagtgcgtt ctttttgatg ctcacgagat tttaaggcac
     4981 gcggcataat taattggcac atttttcctg taataagttc gggtttaatg tgagcagttg
     5041 cggcatactt gtctttgtcg tagtcaatat aatgttcgtt tgcccaaata atatttttgg
     5101 tggttttaac agttgatgct gtgcggtcta ttaataaaat atcaagaata gcagagcgtt
     5161 gttcacggag agtattttta aaaatatcag agacatcatg ctcaaagtga tgttctcgtt
     5221 gttgagccga gtcatttgag cattgaacag taggaaattc aaagctggta ttatatgtcg
     5281 cttttgtaga ggtcaacata tcaaaaaagt catcatcaat aattataagg tttgttcagt
     5341 ataactgagt tatgcacaga cattaagcct aattttatgt tgacagtttt taaaaatctg
     5401 acgaatcaat aagtttaatt gatagaatac gctatattgc accttaatat tggaatcata
     5461 tgaaaaccct ttcccttaaa aaatatgaag accgccgttt acgcgaaggt catt
//
LOCUS       DAOQMB010000126         5485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6667_length_5485_cov_4.684530, whole genome shotgun sequence.
ACCESSION   DAOQMB010000126 DAOQMB010000000
VERSION     DAOQMB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6667_length_5485_cov_4.684530"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="PLY05_05715"
     CDS             <1..1826
                     /locus_tag="PLY05_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HNC02836.1"
                     /translation="FQELGSESAELLLDEELDFSEAPTADFLAAILPANQPKNHAFLY
                     KPVLACTIDHIIAATETTRGGKYILPCLRLLSSDLVIDEVDDFDGQDLIAIGRLIHLA
                     GMLGRKVMISSATIPPSLAEGFFNAYQEGWELYNAFKQQTQPIACIWVDEFKSLIETI
                     NATDSKQRTKHYQKTHQAFISKRIEQLKQQTIKRKGYIVDCEALYQQSTNNIESTEEQ
                     YFSLIQQQIIKLHQQHHTIDKQTGKKVSFGVVRVANIPPCIALSQYLLNVDWDNTISP
                     KIMAYHSRQVLLLRHEQETHLDEVLKRKEKIGEMPHAFNNYAIRQHLSHTNADHVVFI
                     LVATPVEEVGRDHDFDWAVIEPSSYRSIIQLAGRVRRHRKEAITHPNIAIMQYNLKGL
                     RHNNKPSYCRPGFENEKSLRLNNHDLKTLLNEKDLQHAINAIPRIQEANPLRHQEQLA
                     DLEHKAISNSIKNYNAKGAQSLQAWLSEYWWMTALPQQFNRFRASSPDIQLFLVWQDN
                     KAVFGIKDEKGYFIPREGDIFNVKPFVISPQAESRLWLKRDYYDSLCRLIENEEPNTL
                     DTEHTLRYKSLRYGEITIAERDKDKEFVYSDQFGLTLKTQN"
     assembly_gap    1940..2039
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2040..3304
                     /locus_tag="PLY05_05720"
     CDS             <2040..3304
                     /locus_tag="PLY05_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004895765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HNC02837.1"
                     /translation="EKKIASELKGKKNQDDDAILKARTKFQAEADNKYHLISWLEKII
                     AKAKPNITTHPAKFTNAKIKSDVSSFIFLGQKAQDGYLKTGNVNLTYAVDVSGDAATN
                     TIIFELYQLLSKHTSESQTLIDLFESDNNELKTFITNLSLDYEKLKDKALSVFFGDNT
                     SPVTNELIRQVYFPIEGNYHLLSITTASSILFEVKTRVDAFDKWIDGNHIRNVKKENK
                     FHADGFDELLNITEIGFSHNEFTKMGNVSYLNVRNKGIAYLLPSTPPTLQKRDIRLPT
                     YDFFKNSLWHGQFKDNFDALHKLMILDINNINIREGINRRIKFVIDQVLEKVFVIRAS
                     NIGWSETEHYKQLPLAQRIWLDDIHLEQRENQELWLEAVSRSFVGWMVHSYEASQKNN
                     GSVTLSTTESSHIRQFVEEAISQDKEFF"
     gene            3304..4188
                     /gene="csy2"
                     /locus_tag="PLY05_05725"
     CDS             3304..4188
                     /gene="csy2"
                     /locus_tag="PLY05_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HNC02838.1"
                     /translation="MRRFLLIPHIKIHNANAMSSPYTIGFPAMTAWLGAVHALQRYVQ
                     QQGFSDARFLKVGVSCHAFDLQTYKGRGDFVSSIIGTSNPLDKDGNRPAFIEEARCHL
                     TVSLLIEYEGIRKVKLNEFIALLNQSLPRFKMASGDILSVKPIEDLYLNEDESSELRK
                     ISNKLMLGHVLIERRDLMIKSMNSGKDALDALLDHLKLMHRAKQDADGNVTWTTERLE
                     KGWLVPIAVGFQGISDLAIAQNQRDSTIPHRFAESVITLGEFVMPYRLSDIDQMLWQY
                     HVDQSQNLYLCQTNTQTA"
     gene            4200..5213
                     /gene="csy3"
                     /locus_tag="PLY05_05730"
     CDS             4200..5213
                     /gene="csy3"
                     /locus_tag="PLY05_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005217651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HNC02839.1"
                     /translation="MAKVEKMIATVLAFEKKLVPSDGYLYGTTWEKRHQTSTPLKLIE
                     KSVRGTISNRLKDAIKSDPLKLNAEVEKPNLQKVDACALGEHHDTLKLHFTLKVLGGV
                     EKPSACNNEAFNQAYATAANIYVEHEGFTELAKRYAINLANGRYLWRNRVGAEKVEVV
                     ITAGSDTWTFNAHDFSLKNFDSSHANILTLASKIADALSGKIPYLLLNIEAYALVGSA
                     QEVYPSEELVLDKGKGDKSKILYSVNDVAAMHSQKIGNALRTIDTWYPEYAETGFPIA
                     IEPYGAVTNLGKAYRNPKEKADFYTLFDRFAVGEKLANKEQEHYVMAVLVRGGVFGQS
                     GKD"
     gene            5218..>5485
                     /gene="cas6f"
                     /locus_tag="PLY05_05735"
     CDS             5218..>5485
                     /gene="cas6f"
                     /locus_tag="PLY05_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005081865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HNC02840.1"
                     /translation="MKYYQELTLIAQAEVSLYVIWSKLYTQLHLAFVEQKDSQEKITY
                     GVSFPRYRLSSDKGIGFLGDKLRIFAHSTQELEQLNLNQWLARLN"
BASE COUNT         1772 a         1062 c         1036 g         1515 t
ORIGIN      
        1 ctttccaaga gttgggttct gaatctgccg aattactatt agatgaggaa cttgatttta
       61 gtgaagcacc aacagccgac tttttagccg ccattcttcc tgctaaccaa cctaaaaatc
      121 acgctttttt atacaagcct gttttggcgt gtaccattga ccatattatt gctgcgacag
      181 aaaccacacg cggcggtaaa tatattttgc cgtgtttacg tttattgtcc tccgatttag
      241 tgattgatga agtagacgat tttgatggtc aagatttaat tgctataggt cgcttaattc
      301 atttggcagg gatgctaggc cgtaaagtaa tgatttcttc ggctactatt ccgccctctc
      361 ttgccgaagg tttttttaac gcttatcaag agggttggga gttatataat gcgtttaaac
      421 aacaaacaca gcctattgct tgtatttggg ttgatgagtt taaaagcctg attgagacga
      481 ttaacgcaac agacagcaaa caacgcacca aacactatca aaaaacacat caggctttta
      541 ttagcaaacg cattgagcag ctcaaacaac aaaccattaa acgcaaaggc tatattgtgg
      601 actgtgaggc tttgtatcaa caaagcacca ataacatcga atctacagaa gaacagtatt
      661 tttcactcat ccaacaacag attattaaac tgcatcaaca acatcacacc attgataaac
      721 aaacaggcaa aaaagtctct tttggcgtag tgcgtgtggc taacattcca ccgtgtattg
      781 ccctcagtca gtatttactg aatgtcgatt gggacaatac catttcgccc aaaatcatgg
      841 cgtaccatag ccgtcaagtg ttgttattac gccatgaaca agaaacacat ttagatgaag
      901 tactaaaacg caaagaaaaa ataggcgaaa tgccacatgc gttcaataac tacgcgatta
      961 gacaacatct tagccatact aacgccgacc atgttgtttt tattttagtg gctacgcctg
     1021 tagaagaagt tggccgtgac catgattttg attgggcagt gattgagcca tcatcttatc
     1081 gttcgattat tcagttagcg gggcgcgttc gccgacaccg taaagaagcg attactcacc
     1141 ctaacattgc tattatgcaa tacaacctga aaggcttacg ccacaacaat aaacccagtt
     1201 attgcagacc cggctttgaa aatgaaaaga gcttacgcct aaataatcac gacttaaaaa
     1261 cgctattaaa cgaaaaagac ttacaacacg ccattaacgc tattccgcgt attcaagaag
     1321 ctaatccctt acgtcatcaa gagcaactgg ctgatttaga acataaagcg attagtaata
     1381 gcatcaaaaa ctataatgct aaaggcgcgc aaagtttgca agcatggtta agtgaatact
     1441 ggtggatgac tgctctgccc caacaattta accgttttag agcaagctcg cccgatattc
     1501 agttattttt ggtttggcaa gataacaagg ccgtatttgg tatcaaagat gaaaaaggat
     1561 attttattcc gcgtgaaggc gatattttta acgttaagcc ttttgttatt tcgccacaag
     1621 cagaatcacg cttatggtta aaacgtgatt attacgacag tctgtgtcgc ttaattgaaa
     1681 atgaagaacc taacacgcta gatacagaac acactctcag atataaatct ttacgctatg
     1741 gcgaaattac gattgccgaa cgtgataaag acaaagaatt tgtttattcc gaccaatttg
     1801 gcttaacact caaaacacaa aattaaaagg agatgatgat ggaagctatt attaattttc
     1861 tagctgcaca aaaagaggag tttctagaaa aaaagattgc cagtgagcta aaaggcaaaa
     1921 agaatcaaga tgatgatgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2041 agaaaaaaag attgccagtg agctaaaagg caaaaagaat caagatgatg atgcaatact
     2101 gaaagcgcga accaaatttc aagccgaggc tgacaataaa taccatctga tttcttggct
     2161 ggaaaaaatc attgccaaag caaagcctaa cataacaact cacccagcaa aatttactaa
     2221 tgccaaaatt aaaagcgatg tttcatcttt tatttttctt ggacaaaaag cgcaggatgg
     2281 ctatttaaaa acaggcaatg ttaatttaac ttatgcagtc gatgtttctg gcgatgccgc
     2341 aacaaatact atcatctttg agctatatca attactctct aaacatacaa gcgaaagcca
     2401 aacactgatt gatttatttg aatcagataa taacgaacta aaaacgttta tcaccaattt
     2461 atctttggac tacgaaaaat taaaagataa agcattatct gttttctttg gcgataacac
     2521 atcacctgtc acaaatgagc ttatcagaca agtctatttt cctattgagg gaaattatca
     2581 tttattgtcc attacgacag cttcaagcat tttatttgag gtaaaaacgc gcgttgatgc
     2641 ttttgacaaa tggattgacg gaaatcatat aaggaatgtc aaaaaagaaa ataaatttca
     2701 tgctgatggc tttgatgaac tactcaacat cacagaaatt gggtttagtc ataacgaatt
     2761 tactaaaatg ggtaatgtga gttacctaaa cgttagaaat aaaggcatag cttatttact
     2821 cccaagcacc cctcccactt tgcaaaaacg cgatatacgc ctacccactt acgatttttt
     2881 caaaaacagc ttatggcatg gccaatttaa agacaacttt gatgcgctgc ataaactgat
     2941 gattttggat attaacaata tcaatatacg cgagggaatt aataggcgta ttaaatttgt
     3001 gattgaccaa gtgctagaaa aagtctttgt cattagagca agtaatatag gttggtcaga
     3061 aacagaacac tataaacaac tacctttagc ccaacgtatt tggcttgatg acattcactt
     3121 agaacaacgc gaaaaccaag agctttggct agaggcggtc agtcgctcat ttgtgggttg
     3181 gatggtgcat agttatgaag ccagtcaaaa aaataatggc tcagtcaccc ttagcacaac
     3241 agaatctagc catattcgcc agtttgttga agaagcgatt agccaagaca aggagttttt
     3301 ttaatgcgcc gttttttatt gattccacat atcaaaatcc ataatgccaa tgcgatgagc
     3361 agcccttata ccattggctt ccctgcaatg acggcttggt taggtgctgt tcatgcgttg
     3421 cagcgttatg ttcaacaaca aggctttagt gatgcacgtt ttttaaaagt gggggttagc
     3481 tgtcatgcct ttgatttgca aacctataaa ggacgtggcg attttgttag ctcaattatt
     3541 ggcacatcga atcctttaga taaagacggt aatcggcctg cttttattga agaagcgcgt
     3601 tgtcatttaa cagtgtcttt attgattgaa tatgaaggca tacgcaaagt aaaacttaac
     3661 gagtttattg cgctactaaa tcaaagccta ccgcgtttta aaatggccag tggtgacatt
     3721 ctctctgtta aacctattga agacttatac ttaaacgaag atgaatcaag cgaattacgc
     3781 aaaatctcca acaaactcat gctaggtcac gttttaattg aacgccgcga cttaatgatt
     3841 aagtcaatga atagcggaaa agatgcccta gatgctttgc ttgaccactt aaaactcatg
     3901 caccgcgcca aacaagacgc tgacggtaat gtgacatgga caactgaacg cctagaaaaa
     3961 ggttggcttg tgccaattgc cgttggcttt caaggaattt ctgacttagc gattgcccaa
     4021 aatcaacgtg acagcactat cccacatcgt tttgccgaaa gtgttatcac actgggcgaa
     4081 tttgtaatgc cctatcgtct tagcgatata gaccaaatgc tttggcaata ccacgttgac
     4141 caaagtcaaa acttatacct ttgccaaacc aacacacaaa cagcctaagg agatttatca
     4201 tggctaaagt agaaaaaatg attgccaccg ttcttgcctt tgaaaaaaaa ttagttccat
     4261 cagatggtta tttatatggc acaacatggg aaaaacgtca tcaaacatcc acaccactaa
     4321 aactgattga aaagtcggta cgtggcacta tttctaaccg tctaaaagat gcgattaaaa
     4381 gtgaccctct taaactcaat gccgaagttg aaaaacctaa tctccagaaa gtagatgctt
     4441 gtgcattagg tgaacatcac gacacactca aattacactt taccttgaaa gtattaggtg
     4501 gagtcgaaaa gccttctgcc tgtaacaacg aagcctttaa tcaagcctat gctactgctg
     4561 ccaacattta tgttgaacac gaaggcttta ctgaactggc taagcgttat gccattaatc
     4621 ttgctaacgg tcgttatttg tggcgtaacc gtgtgggtgc agaaaaagta gaggttgtaa
     4681 ttacggctgg cagtgataca tggacattta atgcacatga ttttagctta aaaaactttg
     4741 atagcagtca tgcaaatata ttgactcttg ccagcaaaat tgcagacgca ttaagtggca
     4801 aaatacccta cttgctactc aatattgagg cttatgcctt agtagggtca gcacaagaag
     4861 tctatcctag tgaggaatta gtactggata aaggcaaagg cgacaaaagc aaaattttat
     4921 attctgttaa tgatgttgcg gcaatgcact cacaaaaaat tggcaatgcc ttacgcacta
     4981 ttgatacttg gtatcctgaa tatgcagaaa caggttttcc tattgctatt gagccttatg
     5041 gtgcagtcac caacttgggt aaagcctatc gcaatcccaa agaaaaagct gatttttata
     5101 ctttatttga ccgctttgct gttggcgaaa aactcgctaa caaagaacag gaacattatg
     5161 tcatggcagt tttagtgcgt ggtggcgtat ttggccaaag tggcaaggac taatgctatg
     5221 aaatactatc aagagctaac cttgattgct caagcagaag tctctttgta tgtgatttgg
     5281 tcaaaactct acacccaact acaccttgct tttgttgagc aaaaagatag tcaagaaaag
     5341 ataacttatg gtgtttcttt tcctcgctat cgcttaagct ctgataaagg cattggtttt
     5401 ttgggtgata aattgcgtat ttttgcccat tccacacaag agctagagca actcaatctt
     5461 aatcaatggt tggcgcgatt gaacg
//
LOCUS       DAOQMB010000127         5476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6684_length_5476_cov_13.826600, whole genome shotgun sequence.
ACCESSION   DAOQMB010000127 DAOQMB010000000
VERSION     DAOQMB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6684_length_5476_cov_13.826600"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..904)
                     /locus_tag="PLY05_05740"
     CDS             complement(56..904)
                     /locus_tag="PLY05_05740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC02841.1"
                     /translation="MANTDSSSISFTAYYTGEVWRQHGLSADAFKTYQGQALYYLGQP
                     IETLARVVAGMSTQTTLLQRHQIIDYVLTKAIEEQGITQIVEIACGLSPRGVRFCQHY
                     DHIHYIEADLPAMLGHKQKLLAENGLLNDRHRVVGINILEQNSHDALEEVFQRELDPT
                     QKTLVITEGLINYFDYPTISQFWTRLAQSLKQFPQGAYVTDLYPNFKWHPMVKIINSF
                     VFGLAKATQSHVTLHFNSQQAVIDGFKDCGFATTTVHVPEAYYHFLPIPTQRIASLVR
                     VVENWV"
     gene            complement(1019..1948)
                     /locus_tag="PLY05_05745"
     CDS             complement(1019..1948)
                     /locus_tag="PLY05_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02842.1"
                     /translation="MTQQNKKLKQKLLNKTLTAANKALEVAEDIVDMAQETAKSVVDT
                     ASDTARSTLQFTANGAQAIAQLAGGGAKMTAQLASDTAKAATQLAQEAALQMAQNYFS
                     KTTLTISLPESQLNKQLRQMTEQHPYFDDISVSCGSDRLTLHLDGHYQRVLYTVSLDF
                     DVLECKVSHDEQFLLLRHVDTRLETQLRQGRLLTNFALRHASHATAFAIKRLPLVVDP
                     ISFLMERVAGISNEGPHLWRVALHKDLMIELLHNRSWIVDKLLALADSTLIPGLSLIM
                     DSEDMLSKLVNQFEIRSMRVLPNRLELLVGISS"
     gene            complement(2021..2941)
                     /gene="ttcA"
                     /locus_tag="PLY05_05750"
     CDS             complement(2021..2941)
                     /gene="ttcA"
                     /locus_tag="PLY05_05750"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HNC02843.1"
                     /translation="MTVEIFTPDNPPQVKPAPVDESLVADRKSRTEFNKLQKRLRRLV
                     GQAIADYNMIEDGDKIMVCLSGGKDSYTMLDVLLSLQMSAPIKFDIVAVNLDQKQPNF
                     PEDVLPKYLTERGIPHFILEKDTYSIVKELTPEGKTYCAVCSRLRRGSLYGFAQEIGA
                     TKVALGHHRDDIMATLFLNLFHGGKLKAMPPKLLSDDGKNILIRPLAYCEEKDIIEYA
                     RYKAFPIIPCNLCGSQENLQRAKINDMLREWDKKHTGRLESIFRAIQNVAPSQLADRE
                     LFDFENLRSTSQPAAKPRDDRLLDMVNLML"
     gene            complement(2996..3916)
                     /locus_tag="PLY05_05755"
     CDS             complement(2996..3916)
                     /locus_tag="PLY05_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC02844.1"
                     /translation="MDALSHLLDDVHFSGAEYLYVNALQGWQFNLAKQTVFYVVLTGD
                     VKVKVAGQTHKLSTGNIVFMPYGAAHSFFDANFDAPKTQGFNLEPEFKGARYDPVVLG
                     HGHPQALVLGVRCLLDSEMAKPLLSSLPDYILIEDGLNGEGPAWLKLGLEFLALESER
                     FRPGRDTLINRLISMFFIECVRDYVEQLPVQSNTWLNAVRDPYLAPVLSAIHAQPAHP
                     WTVVDLAKLACLSRSSFAERFANMMGQPPLAYLTEYRLRQAARYLTIGDMSVNRVSER
                     VGYSSETAFSQAFKRQYGVSPSQYRKQGRD"
     gene            4184..5431
                     /locus_tag="PLY05_05760"
     CDS             4184..5431
                     /locus_tag="PLY05_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004961710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkane 1-monooxygenase"
                     /protein_id="HNC02845.1"
                     /translation="MNAKTPDHLLAETSQKSKWKDPKRYLWMLSPALPAIGIAALSAY
                     AIAPKKLRALAWVGPALVHGVVPALDRLIGQDQSNPPEDAVKDLENDKYYGRVVKAFV
                     PLQYGVTLMGAYLASRKNTPALDYLGLTLSVGVLNGIGINTAHELGHKHDKAHRLLAM
                     AALAPTAYTHFAVEHNFGHHKRAATPEDPASSRMGESFWKFLPRTVIGGFKSAIEIEK
                     ERLARKGKGFWSVDNELLQGWAMTGGLFAATTLLFGRRSIPFMVGQAAYGASLLEVIN
                     YVEHYGLKREKLANGKYERTAPTHSWNSNHIVTNLVLYQLQRHSDHHAHPSRSFQALR
                     HFEKAPQLPGGYASMLLPAYVPSLWYKLMDERVIKHYNGDLSKINMDPDEQGFLMQKY
                     APLAKRIKEENEQTLIEATPEAA"
BASE COUNT         1665 a         1139 c         1040 g         1632 t
ORIGIN      
        1 cttttaaata aacggttttt gggttagtca ttataaatct ctaaaatatt atttattaca
       61 cccaattctc tactacacgc accaagctgg caatacgttg tgtgggaatg ggtaaaaaat
      121 gataatacgc ttcaggcaca tggacggttg ttgtcgcaaa accgcagtct ttaaaaccat
      181 caatcaccgc ttgttgacta ttaaaatgca aagtcacatg gctttgtgtc gcttttgcca
      241 agccaaaaac aaagctatta ataattttta ccataggatg ccatttaaaa tttgggtaca
      301 aatcggtgac atacgctcct tgtggaaatt gtttgaggct ttgtgctaaa cgtgtccaaa
      361 attgactaat cgtgggatag tcaaaataat taattaagcc ttcggtaatg actaaggttt
      421 tttgtgtcgg gtctaattcg cgttgaaaca cttcttctaa agcatcatgg ctattttgct
      481 ccaaaatatt aatacccacg acacgatgac gatcatttaa caagccattt tcagcaagta
      541 atttttgctt atgccctaac atggcgggca aatccgcttc gatataatga atatggtcat
      601 aatgttggca aaaacgcacc cctcttggtg acaaaccaca agctatttct actatttggg
      661 taatgccttg ttcttcaatg gctttggtca agacataatc aataatttga tgacgttgta
      721 ataaagtggt ttgggtagac atccccgcca caacacgtgc aagggtttca attggttgac
      781 ctaaataata taaggcttga ccttgatagg ttttaaaagc atcagccgac aaaccatgtt
      841 gtcgccaaac ttcacctgtg taataggctg taaaactaat ggaggagcta tcggtgttag
      901 ccattgtttc attctcttat caataatttg ttagttatcg aacttacaaa aagacaagat
      961 aaatggctag agcttttagg gactttttta ttaagactgg cctaaaatga ttaggtattt
     1021 aagagctaat acccaccagt aactctaaac gattaggcaa gactcgcatt gagcgaattt
     1081 caaattgatt cactaattta gacaacatat cttcgctgtc catgattaat gataaaccgg
     1141 gaattaaggt tgaatcggct aaggctaata acttatccac gatccacgaa cgattatgta
     1201 ataattcaat cattaaatct ttatgcaagg ctacgcgcca caagtgcggg ccttcattgc
     1261 taatgcctgc taccctttcc attaaaaaac taatcgggtc aacgaccaac ggtaaacgtt
     1321 taatggcaaa agcggttgca tgagacgcat ggcgcaaggc aaaattggtt aataatcgtc
     1381 cttgccgtaa ttgtgtttct aaacgggtat caacatgacg caataacaaa aactgttcat
     1441 cgtgcgacac cttacactct aagacatcaa aatccaaact caccgtgtat aacacacgct
     1501 gataatggcc atctaaatgc aaagttaaac gatcactgcc acaactgaca ctaatatcat
     1561 caaagtatgg atgttgctcg gtcatttgcc gaagctgttt atttaattgg ctttcaggta
     1621 aggagatggt taaggtcgtt ttactaaaat agttttgcgc catttgcaaa gcggcttctt
     1681 gagcaagctg tgtagctgct ttagccgtat cagacgcaag ctgtgcggtc atctttgcac
     1741 cgccacctgc aagttgggca atcgcttgtg caccatttgc agtaaattgt aaggtactac
     1801 gtgccgtgtc actggcagta tcaaccaccg acttagcagt ttcttgagcc atatcaacaa
     1861 tatcctcagc aacttccaaa gctttatttg cagcagttaa ggttttattt aataactttt
     1921 gcttaagctt tttattttgc tgagtcataa aattttcctt gttgtttcaa gtttttaagt
     1981 cactgtaaca taacagactg tcacagtgta caacaaaact ttagagcatc aaattaacca
     2041 tgtccaacaa tctatcatca cgcggttttg cggcgggttg actggtgctt cttaaatttt
     2101 caaaatcaaa taattcacgg tcagctaatt gagacggggc aacattttga atggctctaa
     2161 aaatactttc taaacggcct gtatgctttt tgtcccactc acgcagcatg tcattaattt
     2221 ttgcccgttg taagttttct tgggagccac acaaattaca gggaataatc gggaatgctt
     2281 tataacgtgc atattcgata atatcttttt cttcgcaata ggctaatggt cgaattaaga
     2341 tatttttgcc atcatcagat aatagcttag gaggcatggc ttttaatttg ccaccatgaa
     2401 acaagtttaa gaataaagtt gccataatgt catcacgatg atgccccaat gcaacctttg
     2461 ttgcgccaat ttcttgggca aaaccatata agctaccacg acgcaaacgt gaacaaacgg
     2521 cacaataggt tttaccttca ggcgttaact ctttaacaat gctataggtg tctttttcta
     2581 aaataaaatg tggaatacca cgttctgtta aatatttagg taacacatct tcgggaaagt
     2641 ttggctgttt ttgatcgagg ttaaccgcca caatgtcaaa ttttatcggc gcgctcattt
     2701 gcaagctcaa taaaacatct aacatggtgt agctatcttt gccacccgat aaacacacca
     2761 taattttgtc gccgtcttca atcatgttgt agtcagcaat ggcttggcca actaaacgac
     2821 gcagacgttt ttgtaattta ttaaactctg tacggctctt acggtcagca accaaagact
     2881 catccacagg tgcaggttta acttgagggg gattatcggg cgtaaaaatt tcaacagtca
     2941 tggcgatcta aacaattatg aaaaaataag cttaatttta cctgatttta actgcctaat
     3001 cacggccttg ttttctgtat tggctaggcg aaactccata ttggcgttta aatgcctgac
     3061 taaaggccgt ttcagaacta tagcccactc gctcactcac ccgattcaca ctcatatcac
     3121 caatggttaa ataacgtgct gcttgacgta agcggtattc ggttaaatag gctaatggcg
     3181 gttgccccat catattggca aaacgctcgg caaaacttga acgcgacaaa caagccaatt
     3241 ttgctaaatc aaccactgtc cacggatgag caggctgagc atgaatcgca ctcagtacag
     3301 gtgctaaata agggtctcgc accgcattaa gccatgtatt actttgtact ggtaattgct
     3361 ctacatagtc ccgcacacat tcaataaaaa acatactaat gaggcgatta attaaggtgt
     3421 ctcgacctgg ccgaaaacgt tcagattcta aggctaaaaa ctctaaaccc aattttaacc
     3481 atgctggccc ttcaccattt aaaccatctt caatcaaaat ataatcaggc aaagacgaca
     3541 acaacggttt ggccatttca ctatctaata aacagcgtac ccctaaaact aaggcttgcg
     3601 gatggccatg ccctaacact acaggatcat aacgtgcgcc tttaaattcg ggctctaaat
     3661 taaaaccctg tgttttgggc gcatcaaaat tagcgtcaaa aaaactatgt gctgcgccat
     3721 aaggcataaa cactatattg cctgtgctta gtttatgggt ttgccctgca actttaactt
     3781 ttacatcacc tgtcaacacc acataaaaga cagtttgctt ggctaaatta aattgccaac
     3841 cttgtaaagc atttacatat aaatattctg caccactaaa atgcacatca tctaatagat
     3901 ggcttaacgc atccatcgtt tatctctctt tatgctttaa attcaaataa ttaaataaat
     3961 ggctttgcca tgctatctat tataatttta tttttttagc caattacaca ccagtaagcc
     4021 gtttttaaac aaaacgactg ttacattact caatgtcggc gtttaacgca agtttagcgg
     4081 ctttttgcaa ctggccgctt tgctctttag gatgcaatat atccctacac agtgtccaac
     4141 aattttcaca caatggcaaa tgccaaatta aaaggtgagc aatatgaacg caaaaacacc
     4201 agatcattta cttgctgaaa ccagtcaaaa aagcaaatgg aaagatccaa aacgttattt
     4261 atggatgtta agtcctgctt tacctgcaat tggtattgca gcactttctg cgtatgcgat
     4321 tgccccaaaa aaattacgtg ctttagcatg ggttggccca gcgttggttc atggtgttgt
     4381 gcctgcttta gatcgtttaa ttggtcaaga tcaaagcaat ccaccagaag atgcggtaaa
     4441 agatttagaa aatgacaaat attatggccg tgtggttaaa gcctttgtgc ctttgcaata
     4501 tggtgtaact ttgatgggtg cgtacttagc tagccgcaaa aacaccccag ctttagatta
     4561 tttgggttta acattatctg ttggtgtgct aaatggtatt ggcattaaca ccgcccatga
     4621 attaggccat aaacacgaca aagcacatcg cttattagca atggccgcat tggctcctac
     4681 tgcatacaca cattttgcgg tagaacataa ctttggtcat cacaaacgtg ctgccacgcc
     4741 agaagaccca gcaagtagcc gtatgggtga aagcttttgg aagtttttac cacgtaccgt
     4801 gattggtggt tttaaatcgg caatcgaaat tgaaaaagag cgtttagcac gtaaaggcaa
     4861 aggtttctgg tcggttgata acgaattatt gcaaggttgg gcaatgacag gtggtttatt
     4921 tgccgccact actttgttgt ttggtcgtcg ttctattcca tttatggttg gtcaagcggc
     4981 ttatggtgca agcttgttag aagtcattaa ttatgtagaa cattatggtt taaaacgcga
     5041 aaaattagcc aatggcaaat atgagcgtac tgcccccaca catagttgga acagcaacca
     5101 tattgtgact aatttagtgt tatatcaatt gcaacgtcac tctgaccatc atgctcatcc
     5161 aagccgtagc ttccaagcct tacgtcactt tgagaaagca ccacaattac ccggtggtta
     5221 tgcgtcgatg ttgttacctg cctatgtgcc ttctttgtgg tacaagttaa tggatgagcg
     5281 cgtaattaaa cactacaacg gtgatttaag caaaatcaat atggatcctg atgagcaagg
     5341 ctttttgatg caaaaatatg cgccattagc taagcgtatt aaagaagaaa acgagcaaac
     5401 gttgattgaa gcaacgccag aagcggctta aatctttcac tctcttgaga cttaaatgtt
     5461 agtttaagtc cctcat
//
LOCUS       DAOQMB010000128         5448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6744_length_5448_cov_4.286297, whole genome shotgun sequence.
ACCESSION   DAOQMB010000128 DAOQMB010000000
VERSION     DAOQMB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6744_length_5448_cov_4.286297"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLY05_05765"
     CDS             <1..350
                     /locus_tag="PLY05_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNC02846.1"
                     /translation="NSFNSYPMDKVAIAGAVAAFEDKAYFQQTCQAIIDGRIWLTAQL
                     EQLGFEVLPSAANFVFARHPQQDAAQLAQALREQAIIVRHFKQTRIEQFLRITIGKKW
                     DNQELVRVLLTLL"
     gene            613..1287
                     /locus_tag="PLY05_05770"
     CDS             613..1287
                     /locus_tag="PLY05_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02847.1"
                     /translation="MKKIILLTYLMLHSVLVFAADINKIIETGAWQLIKDDSVHKIKV
                     YGREENKFNYRTFKAEAILNAPLDHVLQSQLDIANYPKWVWSAIEAKALKKVADNEFY
                     YHLVSRTPLIPNRDTIVHTKINPYTQAKPSIELIMQGTPDYYPPQKDMVRVKVYQAVI
                     KLTPQGDQTLINYQGYIELGDDAPAWALNNYQKLAPHLSMLGLRRLLQSSLYKNKNFP
                     SPLIVR"
     gene            1495..3306
                     /locus_tag="PLY05_05775"
     CDS             1495..3306
                     /locus_tag="PLY05_05775"
                     /inference="COORDINATES: protein motif:HMM:NF018662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 domain-containing protein"
                     /protein_id="HNC02848.1"
                     /translation="MKYNKKLCLSPLFIAIAMSSFSPVYALEVPMNNEDINIRWDNTF
                     RYNYAHRIEAQDEALLKNPNFDDGNRNFDKGMVSNRLDILSELDVVYQNKHGVRLSMA
                     GWYDDAYNQGFDNKNVATSNHLEDGKAAIGVPNFVERYYQGPSGEILDAFVFTNVELA
                     EMPINIKAGRHTVYWGESLLFSGALHGVSYGQAAVDAAKGFAVPGTEVKELFRPLNQI
                     SAQMRIKPDLSLAMQYFFDWERYRLPDSGTYLGFNDALHESGESLISSGARLVRGEDS
                     APSGKGDMGLALRWSPEFLDGSVGFYVRRFSDTLPQLHVRPAVAALPAANCAALGFVT
                     LPTGACYINPSAASPTDLAAGRIGTYHLAYGDEVDLLGFSLAKGIAGVSVGIDLSYRK
                     NMPLTSDIVNILPTQLIAATGNKAAINYVPTDGETGGARGDTLHGVVNLLGVVGTTPM
                     WNQLTWATELTWNRWQKVTQNEAVFKGRDNYSVMVDGVPSTLDKVSKDFFGIAANVTP
                     MWYQVLPAVDLYLPLSVSVGLSGNSAVSLGGNEKSGTYSIGVGADIQQRYRVDLKFID
                     FLGEYQTNAAGMVQISNGNPTQLKDRGMVTLTFKTTL"
     gene            3365..4705
                     /locus_tag="PLY05_05780"
     CDS             3365..4705
                     /locus_tag="PLY05_05780"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HNC02849.1"
                     /translation="MKFSLTALMAALLCMNQANAAVSANEASQLGQKLTAIGAEKAGN
                     AEKTIPAYTGGLNKVPAGLRVTNGKRPSPFDEEKPSLVINSQNAATYSGKLSAGTQLL
                     FKRYPDFRMEVYPTYRTVAFPTAVSEATRKCAETAELTNNNNAVKGAKACIPFPIPKT
                     GAEAIWNHKLRYDGKSYEFNYEAYNVDRSGRAVMASKGENIVYYPFQDASNKTDQLLN
                     FWINYTGPARRAGERLLVIDPLDDVAVGRRAWQYMPGQRRTKLAPNVAYDAPNPSTAG
                     ASTYDDALMYNGAIDRYDWKLVGKKEMYVPYNNYKLSYFSTPEEALKPNFINPDVVRW
                     ELHRVWEVEASLKPNKRHVYSKRTFYLDEDSWIILASDQYDARGQLYRSGFAFMTMSY
                     DEVAPFTSNYVHYDFIAQMYAMNFWPSCSRCGLKYVPISPTIFTPDNMAGTGIR"
     gene            4846..>5448
                     /locus_tag="PLY05_05785"
     CDS             4846..>5448
                     /locus_tag="PLY05_05785"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HNC02850.1"
                     /translation="MTAITIKISLLVASLVAGHVTYAATSPDVLNTPAMMTQIATDKP
                     LFALAKAEQRLIAVGQRGHILYSDNQGAVWQQAKVPVSSDLTAVYFVSANKGWAVGHD
                     GVILVTNDKGQTWQKQLDGWQAAEIFKKQAASLNPEQHQSLMTELERIASQGADKSFL
                     DVWFANENMGFVVGAFGLALQTKDGGKTWQSLMAQVQNPNF"
BASE COUNT         1650 a          902 c         1198 g         1698 t
ORIGIN      
        1 aaaatagctt taactcttat ccaatggata aagtggcgat tgctggcgca gtggctgcat
       61 ttgaagataa agcctatttt cagcaaactt gccaagcgat tatagatggt cgtatatggt
      121 taaccgcgca attagagcaa ttgggttttg aggttttacc ctcagccgct aattttgtct
      181 ttgcgcgtca tccacagcaa gatgccgcac aattagctca agctttgcgt gaacaagcga
      241 ttattgttcg tcatttcaaa cagactcgga ttgaacagtt tttaaggatt actattggca
      301 aaaaatggga taatcaggaa ttagttaggg ttttattaac gcttttgtaa aacttggact
      361 gtcgtccaga aaaaagacga tcaaacctat tttaaggttg catcgtcttt ttttttacgg
      421 tattgtttgt gcagcttaca acatggccaa taggtatcct ctaactatct ctacgagagc
      481 ctaaagagtt tgattaagtg tcatatgttt gttggttata aatggttaga tcacttagtg
      541 atgtgataac acaacataca atttcgcata tagaaaagat aaataaattt gaaaattgta
      601 ggttatttaa aaatgaaaaa aattatttta ttaacgtatt tgatgttaca ttcagtatta
      661 gtttttgcgg cagatataaa taaaatcatt gaaacgggtg catggcaatt aattaaagat
      721 gatagtgttc ataaaataaa agtatatggc cgagaagaaa ataaatttaa ttatcgtacc
      781 tttaaagcag aagcaatatt aaatgcacct ttggatcatg ttttacaatc tcaattagat
      841 attgctaatt atccaaaatg ggtatggtcg gcaattgaag caaaagcatt aaaaaaggtg
      901 gcagataatg aattttatta tcatctggta tctcgtacgc cattaattcc taatagagat
      961 accattgtac atactaaaat caatccatat acgcaagcta aaccttcgat tgaattaatt
     1021 atgcaaggta cgcccgatta ttatccgcca caaaaagata tggttagggt taaagtttat
     1081 caagcagtga ttaaattaac gcctcaaggc gatcaaaccc tgattaatta tcaaggttat
     1141 attgaacttg gggatgacgc accagcatgg gcattaaata attaccaaaa attagcccca
     1201 catttgtcta tgttaggttt aagacgcttg ttacagagta gcctgtataa aaataaaaat
     1261 tttccatcac cattgattgt gcggtgatct agtctaaaat aaaaaatgct tagcctataa
     1321 atataaacta agctaaataa ttattatttt tttgtctttg gcaaaaaata taaataacga
     1381 gttgagaata tctaaactca atcaaaccac aataatattt atcaaaaaat aaatgtttta
     1441 agcaatagct gaaaggttat tgtttgacca ttttttatag ggggtagtta gctaatgaaa
     1501 tacaataaaa aattgtgttt atcgccatta ttcattgcca ttgctatgag tagtttcagt
     1561 cctgtttatg cccttgaagt gcctatgaat aatgaggata ttaatattcg ttgggataat
     1621 acctttcgtt ataattatgc gcatcgtata gaagcgcaag atgaggcatt attaaaaaat
     1681 cctaattttg atgatggtaa tcgtaatttt gataaaggca tggtgtctaa tcgtttagat
     1741 attttatctg agcttgatgt ggtttatcaa aacaaacatg gtgtacgttt gagtatggct
     1801 ggttggtatg acgatgctta taatcaaggt tttgataata aaaatgtggc tacctctaat
     1861 catttagaag atggcaaagc agccatcgga gttcccaact ttgttgagcg ttattatcaa
     1921 gggccatcgg gtgagatttt agatgccttt gtctttacca atgtcgaatt agctgagatg
     1981 ccaattaata ttaaagcagg tcgtcacacg gtgtattggg gtgaaagttt attatttagt
     2041 ggcgcgttac atggtgtgtc ctatggacaa gcagccgttg atgctgctaa gggttttgct
     2101 gtaccaggta cagaagtcaa agagttattt agaccattaa atcaaatttc ggcacaaatg
     2161 cgcatcaaac ctgatttgtc tttggcgatg caatactttt ttgattggga gcgttatcgt
     2221 ttacccgact caggtaccta tttaggtttt aatgatgcac tgcatgagag tggtgaatcc
     2281 ttaatcagca gtggtgcgcg tttggtaagg ggcgaagata gtgcgccatc gggtaaaggg
     2341 gatatgggtt tggctttacg ctggagtcct gaatttttag atggtagtgt cggattttat
     2401 gtacggcggt tttctgatac cttaccacag cttcatgtgc gtcctgcagt cgcggcctta
     2461 cctgctgcaa attgtgcagc tttaggtttt gttaccttac cgacaggagc ttgttatatt
     2521 aatccgtcag ctgctagccc aaccgattta gcggcagggc gtattggaac ttatcattta
     2581 gcgtacggtg atgaggttga tttattaggt tttagtttgg ctaaaggcat tgcaggggtg
     2641 agtgttggta ttgatttgtc ttatcgtaaa aatatgcctt taaccagtga tattgtgaat
     2701 attctgccta cacaattaat tgctgcaaca ggtaataaag cggctattaa ttatgtgcct
     2761 actgatggtg aaacaggtgg tgcacgtggt gataccttac atggcgtggt caatttgttg
     2821 ggggtggttg gcacaacccc gatgtggaat caattaacgt gggccaccga attaacatgg
     2881 aatcgttggc aaaaggtgac tcaaaatgaa gctgtattta aaggtcgtga taattacagt
     2941 gtcatggttg atggtgtgcc ttcaacgctt gataaagtca gcaaagactt ttttggtata
     3001 gccgccaatg tcaccccaat gtggtatcaa gtattgcccg cagttgattt gtatttacca
     3061 ctgagtgttt ctgtgggttt aagtggtaat tcagcagtca gtttaggtgg taacgaaaaa
     3121 tcaggcacgt atagtattgg tgtaggtgcg gatattcaac aacgttatcg ggttgattta
     3181 aagtttatcg actttttggg cgaataccaa accaatgcgg cgggtatggt gcaaatttcc
     3241 aatggtaatc caacacagtt aaaagaccgt ggtatggtga cactgacgtt taaaacaaca
     3301 ttataaaaca caataaggac tgaggaagaa cacagtccta agacaaaaaa ttaagaggtt
     3361 tattatgaaa ttttcgttaa cggcattaat ggccgctttg ttgtgtatga atcaggccaa
     3421 tgccgcagta tcggcaaatg aagccagtca gcttggccaa aaattgacag ctattggggc
     3481 agaaaaagca ggtaatgccg aaaaaactat tcctgcttat acaggtggtt taaataaagt
     3541 gcctgcgggt ttacgcgtga ctaatggtaa acgtcccagc ccttttgatg aagaaaagcc
     3601 tagtttagta attaatagcc aaaatgcagc gacttacagt ggcaaattga gtgcgggtac
     3661 acaattatta tttaaacgtt accccgattt tagaatggaa gtttatccaa catatcgcac
     3721 agtggctttt ccaacagccg tatctgaagc aacgcgtaaa tgtgccgaaa cagccgaatt
     3781 aaccaataat aataatgcgg ttaaaggagc aaaagcgtgt attcctttcc caattcctaa
     3841 aacaggtgca gaggctattt ggaatcataa attgcgctat gatggcaaaa gttatgagtt
     3901 taactatgaa gcttataatg ttgataggtc cggccgtgca gtaatggctt caaaaggtga
     3961 aaatattgtt tattatccct ttcaggatgc cagtaataaa accgatcaat tgttaaactt
     4021 ttggattaat tatacagggc cagcacgccg tgcaggggaa agattgttgg tgattgatcc
     4081 tttagatgac gttgctgttg gtcgtcgtgc ttggcaatat atgcctggcc agcgtcgtac
     4141 taaactcgca cctaacgtgg cttatgatgc gcccaatccc tctaccgcag gggcatcaac
     4201 ctatgatgat gctttaatgt ataacggtgc gattgatcgt tatgactgga agttagttgg
     4261 caaaaaagaa atgtatgtgc catacaataa ctataagtta agctatttta gtacccccga
     4321 agaagcgtta aagcccaatt ttattaatcc tgatgtggtt cgttgggagt tacaccgcgt
     4381 atgggaagtt gaggcaagct taaaacccaa taaacgccac gtttacagta aacgaacttt
     4441 ttatttagat gaagatagtt ggatcatttt agcaagtgac caatatgatg cgcgtggtca
     4501 attatatcgt tctggttttg cgtttatgac catgagttat gatgaagttg cgccatttac
     4561 ctcaaattat gtgcattatg attttatcgc acagatgtac gccatgaatt tttggccaag
     4621 ttgtagtcgt tgtggtttga aatatgtgcc aatttcgcca acaattttta cgcctgataa
     4681 catggcgggt acaggtattc gttgattata ttttaactct tctacgagga gagggagctt
     4741 catcgctaat accttgtttt gcgatgaaag tcccctatcc ctgtggaata gtgttagggt
     4801 gagggcttta gatggctttt gtatccattt tgaggaataa cgataatgac cgctataact
     4861 attaaaatat ctttactggt cgcgtctctt gttgctggtc atgttacgta tgctgcgact
     4921 tcgcctgatg tattaaatac cccagccatg atgactcaga tagcaacaga taaaccttta
     4981 tttgctttgg ccaaagccga acaacgatta attgcggttg gtcaacgtgg tcacattttg
     5041 tattcagata atcaaggtgc tgtttggcaa caagcgaaag tgcctgtcag ttccgattta
     5101 acggctgttt attttgtctc ggccaacaaa ggttgggcgg taggccatga tggagtgatt
     5161 ttagttacca acgataaagg acaaacatgg caaaaacagt tagatggttg gcaggctgct
     5221 gaaattttta aaaaacaagc agccagttta aacccagaac aacatcaatc attaatgact
     5281 gagttagagc gtattgctag tcaaggtgcg gataagtcat ttttagatgt atggtttgcc
     5341 aatgaaaata tgggttttgt agtgggtgca tttggtttgg cattacaaac taaagatggt
     5401 ggcaaaacat ggcaatcact gatggcacaa gtacaaaatc ccaatttt
//
LOCUS       DAOQMB010000129         5433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6772_length_5433_cov_12.519524, whole genome shotgun sequence.
ACCESSION   DAOQMB010000129 DAOQMB010000000
VERSION     DAOQMB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6772_length_5433_cov_12.519524"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="PLY05_05790"
     CDS             complement(<1..305)
                     /locus_tag="PLY05_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02851.1"
                     /translation="MRAEKTPLLPETWDDLEKILKQKAELALDRIEQALQKVDETVAL
                     VKAVGQDSAQLAVKEAKEAFLLAKRVDVEKNIKDLRKQAEDRIQEFADSLEQRREQE"
     gene            complement(315..1331)
                     /gene="hemH"
                     /locus_tag="PLY05_05795"
     CDS             complement(315..1331)
                     /gene="hemH"
                     /locus_tag="PLY05_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HNC02852.1"
                     /translation="MKANSAPPLGVVLVNLGTPDAPTPQAVRRYLRQFLSDRRVIEIP
                     PIVWQIILNLFILPFRPKRVAQLYAAIWQQDSPMRAILQQQSQLLAVQLQDVVPTQVI
                     VKAAMSYGNPSLPSALNQLRREGVEHIVVLPLFPQYSATSTAPVYDAMQRWCATQRNL
                     PSFTIIKDYFAHPQYIAAMANSIREFRQQHGTAQKLLMSFHGIPQPYADKGDPYPKRC
                     RCTAAHIAHILGLKDDEWAISFQSRFGKQEWVKPYTDVLLAEWAKQGVESVQIVSPAF
                     SADCLETLEELAEENKHLFLDAGGKQYHYIPALNQRADHIELLAQLVTPHLQAFWQTM
                     PYYH"
     gene            complement(1497..2150)
                     /gene="adk"
                     /locus_tag="PLY05_05800"
     CDS             complement(1497..2150)
                     /gene="adk"
                     /locus_tag="PLY05_05800"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNC02853.1"
                     /translation="MRVILLGPPGAGKGTQAQVITQQFQIPQISTGDMLRAAVKAGTE
                     LGVAAKKIMDAGGLVSDEIIIGLVKERLTQPDCVNGCLFDGFPRTIPQAQALINAGIA
                     IDHVVEIAVPDQEIISRLSGRRVHPNSGRVYHIQHNPPKVDGKDDVTGEDLVQRDDDK
                     EETIKKRLEVYHQQTAQLVGFYQQLAASGDTTAPKYARIEGVGSVEAISAKVTSVLK"
     gene            complement(2211..3488)
                     /gene="ccmI"
                     /locus_tag="PLY05_05805"
     CDS             complement(2211..3488)
                     /gene="ccmI"
                     /locus_tag="PLY05_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HNC02854.1"
                     /translation="MINSYSLAFISSAAALSFIVALVFIWPLLRSAREQNSSLLALNI
                     DVFKERLAELEQDKLDGKIDEETFVALKTELERQLLSLAEQMEQKNQQQASSSRGLIW
                     SLGLLIPLLGAGAYWTFMPHNQIWRWWQVQTETEPLINDLFANKQIEPERLAKQNLAD
                     FARVMQKQLQTNPNNVDGWYMLAMAYIQGELPDLALTALANANRLDPARDDIALTYAQ
                     TLVFTQQGQLSTKSRELLMQVLNKHPEHEGALLLMGMGAYRSGDYASALVFLPRLKQV
                     HIKRTGESQSDALTEIDKAIAIAQQGGEKLAENQTGIEVTVKLAKELQSRLQPNDTLF
                     VFARALNGSPIPLAVVRQPVSRFPLVVALTDKQSMMPTMKLSTVESVVVGARISKTGT
                     PQGASGDLEAIAVPVTQNGKLQHIELLINQVKP"
     gene            complement(3481..3942)
                     /locus_tag="PLY05_05810"
     CDS             complement(3481..3942)
                     /locus_tag="PLY05_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003172775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HNC02855.1"
                     /translation="MSIKAMIVILLSLYLPISYAAIEVYDFKTPAEEQRFQALIGELR
                     CPKCQNQNLSGSDAPIAKDLKDRTYQLLQEGKTDNEIRDYMVDRYGDFISYKPPVRST
                     TYLLWFGPFILLVMVVLIIIFRRRQVKPDASALTPEESARLAALLKRNSHD"
     gene            complement(3933..4484)
                     /locus_tag="PLY05_05815"
     CDS             complement(3933..4484)
                     /locus_tag="PLY05_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HNC02856.1"
                     /translation="MKMKALVWAFPLVLFLAVAAFLMTRLGQDPTALPSARLGKPFPN
                     FAVTDLDGKPVSLADIKGKPAIINVWASWCPSCKEEHPTLVELSKQGIPIIGLNYKDD
                     PQDALAYLAKRGNPFSLIIPDRKGDIGIDLGVYGAPETYLIDAQANVRYRYVGVFDAT
                     IWQTQLAPCYQQLLAGGEATACQ"
     gene            complement(4481..>5433)
                     /locus_tag="PLY05_05820"
     CDS             complement(4481..>5433)
                     /locus_tag="PLY05_05820"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HNC02857.1"
                     /translation="YAWLSRESFLLVNNLLLITATTVVLLGTLFPLVADALKLGKISV
                     GPPYFNALFVPVTLVLLVFLGLGSLVNWKRHQAKEFLPLWKVLLASIVLGIVFPLAYF
                     GNAPIRVALSLSLCFWVILSITRDIYLKAKTAKQGFGAGLLRLSRSYYGMQIAHLGLV
                     ITVIGVTLTSTYSVEKDVRLGMNDKVDVGAYQFEWQGVREYSGANFESVQAKVAVLKN
                     GEVIEYLYPEKRLYVVQNRSMTEAAIDGRLLRDVYVAIGEPLDNNQWAVRVYYKPFVR
                     WLWLGALVMALGGGVAMTDKRYRLVRQNAKVSNSAQVATK"
BASE COUNT         1676 a         1181 c          995 g         1581 t
ORIGIN      
        1 atttcttgct ctcggcgttg ctctaatgaa tcggcaaatt cttgtatgcg gtcttctgct
       61 tgtttgcgta aatctttaat atttttttct acatcaacac gtttagccaa taaaaaagct
      121 tcttttgcct ctttaacggc taattgtgct gaatcttggc caacagcttt aaccaaagcc
      181 actgtttcat caactttttg tagggcttgt tcaatacgat caagagccaa ttcagctttt
      241 tgtttgagta ttttttctaa atcatcccat gtttcgggca acaaaggcgt tttttctgca
      301 cgcataacaa gtccttagtg ataatatggc atagtttgcc aaaaagcctg caagtgtggt
      361 gtgactaatt gtgctaataa ctctatatga tctgcgcgtt gatttaaagc aggaatatag
      421 tgatattgtt tgccacctgc gtctaaaaat aaatgtttat tttcttctgc taactcttct
      481 aaagtttcta aacaatctgc tgaaaatgca ggagaaacga tttgtaccga ctctacacct
      541 tgttttgccc actccgccaa taaaacatcc gtataaggtt tcacccattc ttgtttacca
      601 aaccgtgact gaaaactaat cgcccactca tcgtctttta aacccaagat atgagcaata
      661 tgtgcggctg tacaacggca acgtttagga tacggatcgc ctttatcggc gtaaggttgt
      721 ggaatgccat gaaacgacat taataatttt tgtgctgtac catgttgctg acgaaattca
      781 cgaatactat tagccattgc tgcgatatat tgaggatgag caaaatagtc tttaataatg
      841 gtaaaactgg gtaaattgcg ctgtgtggca caccaacgct gcatggcatc atatacaggc
      901 gcggtggacg ttgccgaata ttgaggaaat aagggcaaca ccacaatatg ctccacacct
      961 tcacggcgta attgatttaa agcagatggt aaactgggat taccataact catggctgct
     1021 ttaacaatca cttgtgttgg cacaacatcc tgtaattgca cggctaataa ttgactttgt
     1081 tgttgcaaaa tagcacgcat tggcgaatct tgttgccaaa tagccgcata gagttgtgcc
     1141 actctttttg gacgaaaagg caaaataaat aaatttaaaa taatctgcca aacaataggc
     1201 ggaatctcaa tcacccgccg atcactcaaa aactgtcgca aataacgccg cacagcttga
     1261 ggagttggcg catcaggtgt gcctaaatta acaagcacaa cgcctaaggg gggtgctgaa
     1321 tttgctttca ttattgcctc ttacattcgt atcttaaaat tccctctgct aatttaggag
     1381 agggttaggg tgaggttatg tgtcgcttcg acaattgaac gttaattcag attatttgtc
     1441 tctagcacaa aataaaaaag gccgttaatc ggcctttttt tgtgcataga cacgtattat
     1501 tttaatacag aagtgacttt agcactaatt gcttcaacag agccaacacc ttcaatacgt
     1561 gcgtattttg gtgcagtggt gtcaccactc gcggctaatt gttgatagaa gcccactaat
     1621 tgtgccgttt gctgatgata aacttctaaa cgttttttaa tagtttcttc tttatcgtca
     1681 tcacgttgta cgaggtcttc gccagttaca tcgtctttac catccacttt aggaggattg
     1741 tgttgaatgt gatacacacg gccagaattt ggatgtacac gacgaccaga caagcggctg
     1801 ataatttctt gatcaggcac agcaatttca acaacgtggt caatcgcaat acctgcatta
     1861 attaaggctt gagcttgagg aatagtacgt ggaaaaccgt caaataaaca accattgaca
     1921 caatctggct gagttaaacg ttcttttact aaaccaatga taatttcatc agaaaccaaa
     1981 ccacccgcat ccataatttt tttagcagca acacccaatt cagtgcctgc ttttactgcg
     2041 gcacgcaaca tgtcgcctgt agaaatttgt ggaatttgaa actgttgagt aattacttgg
     2101 gcttgagtgc cttttcctgc accaggtggg cctaataaaa taacacgcat aatgctcccc
     2161 taatttaaaa taaaaagcaa aaattttgcc aagtttacca taaaaattat ttacggcttt
     2221 acttgattaa ttaacaattc aatatgttgt agtttgccgt tttgtgtgac aggcactgct
     2281 atcgcttcta aatcaccgct agctccttgt ggtgtgcctg ttttgctaat tcttgctccc
     2341 accacaaccg actcaactgt ggatagtttc atggtcggca tcatactttg tttatcagtt
     2401 aatgcaacta ctagcggaaa acggctgaca ggctgacgaa cgactgccaa aggtattggt
     2461 gaaccattta aagcacgagc aaaaacaaat aaagtatcat ttggctgtaa acgagactgc
     2521 aattcttttg ccaatttgac tgtcacttcg atgcctgttt gattttcggc taacttttca
     2581 ccaccttgtt gagcaatggc aatggctttg tcgatttccg ttaaagcatc agattgactt
     2641 tcacccgtac gtttaatatg tacttgtttt aaacgcggca aaaacaccaa agcactggca
     2701 taatcacctg agcgataagc gcccataccc attaacaaca atgcgccttc gtgttctgga
     2761 tgcttattta acacctgcat caataattct cgacttttgg tgctaagttg tccttgttga
     2821 gtaaatacca aagtttgcgc ataggttaag gcaatatcat cacgcgcagg gtctaaacga
     2881 ttggcattgg ctaaagcagt taaggctaaa tcgggtaatt caccttgaat ataagccatc
     2941 gccaacatat accaaccgtc aacattatta gggttagttt gcaattgctt ttgcatcaca
     3001 cgagcaaaat ctgccaaatt ttgtttggct aaacgttcag gctcaatttg tttgttagca
     3061 aataaatcat taatcagtgg ctcagtttcg gtttgtactt gccaccaacg ccaaatttga
     3121 ttatgtggca taaatgtcca atacgcacca gcacccaata atggaattaa caaacctaaa
     3181 gaccaaataa gccctcgtga actactcgct tgctgctgat ttttttgctc catttgttcg
     3241 gctaatgata ataattgacg ctctaattct gtttttaatg caacaaatgt ttcttcatca
     3301 attttaccat caagtttatc ttgctctaat tctgccaaac gctctttaaa aacatcaata
     3361 tttaatgcta ataacgagct attttgttca cgtgctgaac gtaataacgg ccatataaaa
     3421 actaaagcaa cgatgaagct taatgcggct gcactactaa taaaggctaa agaatatgag
     3481 ttaatcatga ctatttcgct ttaataaagc cgctagacgg gcagactctt cgggcgttaa
     3541 tgcggaggca tcaggtttaa cttggcgacg acggaaaata ataatcaaaa ccaccatcac
     3601 taataaaata aatggcccaa accacaataa ataggttgtt gaacggacag gcggtttata
     3661 gctaataaag tctccataac gatcaaccat atagtcacga atttcgttat ccgttttgcc
     3721 ttcttgtaac agttgataag tacgatcttt taaatctttg gcaatgggtg catctgagcc
     3781 tgataagttt tgattttgac actttggaca acgtaattcg ccaatgagtg cctgaaaacg
     3841 ctgttcttca gcgggcgttt taaaatcata tacctcaatt gctgcataac ttattggcaa
     3901 atagagactg agcaaaatca caatcatcgc ttttattgac atgctgttgc ctccccacct
     3961 gctaataatt gctgataaca aggggctaat tgtgtttgcc aaattgttgc atcaaacaca
     4021 cccacataac gatagcgaac attggcttgt gcatcgatta aataggtttc aggtgcgcca
     4081 tacacgccta aatcaatccc aatatcgcct ttacgatcag gaataatcaa actaaaagga
     4141 ttgccacgtt tagccaaata agccaaagca tcttgtggat catctttgta attcagaccg
     4201 ataatcggaa taccttgttt agacaattca actaaagttg gatgctcttc tttacaagaa
     4261 ggacaccatg acgcccaaac attaataatc gcgggtttgc ctttgatgtc tgctaaagaa
     4321 acaggcttac cgtctaaatc agtcacggca aaattgggaa atggcttgcc taaacgtgct
     4381 gaaggtaaag cggttgggtc ttgtcctaaa cgtgtcatca aaaaggcagc aactgctaaa
     4441 aataaaacaa gggggaacgc ccataccagt gccttcattt tcattttgtc gctacctgtg
     4501 ctgaattaga gacttttgcg ttttggcgca ctaaacgata acgcttatca gtcattgcta
     4561 caccgccacc caaagccatc accaaagcac ctaaccataa ccagcgaaca aatggtttat
     4621 aataaacacg aactgcccat tggttattgt ctaatggctc acctatcgcc acatacacat
     4681 cacgcaacaa acggccatca atcgctgcct cagtcataga gcgattttgc accacatata
     4741 aacgcttttc gggatataaa tactcaatca cttcgccatt ttttaaaaca gcaactttgg
     4801 cttgtaccga ttcaaaatta gccccactat attcgcgcac accttgccat tcaaattgat
     4861 atgcaccgac atcaacttta tcattcatgc ctaaacgtac atctttttcg acactatacg
     4921 ttgaagtcaa ggtaacacca ataacggtaa ttactaatcc caagtgggca atttgcatac
     4981 cataataact acgcgacaaa cgtaataaac ctgcgccaaa accctgcttc gctgttttag
     5041 ccttaaggta aatatcgcgt gtaatggata aaatcaccca aaaacataaa ctcaaactca
     5101 aagccacacg aatgggggca ttaccaaaat aagctaaggg aaaaacaata cccaaaacaa
     5161 tactcgctaa tagaactttc cataggggca aaaactcttt ggcttgatgc cgcttccagt
     5221 taaccaaaga gcccaagccc aaaaagacta ataacactaa ggtaacaggc acaaataagg
     5281 cattaaaata gggagggccg accgaaattt tgcctaattt taaggcatca gctactaagg
     5341 gaaataacgt acctaataac acgacagttg ttgccgtaat taataataag ttattaacta
     5401 ataaaaaaga ttctcgtgat aaccacgcat aac
//
LOCUS       DAOQMB010000130         5427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6790_length_5427_cov_14.871556, whole genome shotgun sequence.
ACCESSION   DAOQMB010000130 DAOQMB010000000
VERSION     DAOQMB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6790_length_5427_cov_14.871556"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..848
                     /locus_tag="PLY05_05825"
     CDS             147..848
                     /locus_tag="PLY05_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC02858.1"
                     /translation="MSLLTVKNLQIAYGGIQAVKGISFEIKQGELVSLIGANGAGKTS
                     TLNCLGGLLKPASGEISYQGQNLLTIPTHDRVKHGLALVPEGRGIFSKLTVAENLRMG
                     AYHRQLGKDELEQAYQRFPRLHERKEQLAGTLSGGEQQMLAIVRALLSKPKLLLLDEP
                     SMGLAPIMVEKIFSTISEVTKEGVAVLLVEQNAKAALALAQRAYVMESGVISLAGDAK
                     DLAADPRVRAAYLGE"
     gene            848..1147
                     /locus_tag="PLY05_05830"
     CDS             848..1147
                     /locus_tag="PLY05_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02859.1"
                     /translation="MIKKVLASMFMGYLVFVLVMWFFSAQQIKQTCQQIPMSLPVSKL
                     EPILDSLYLTKIMRRSSEQKIQTILLHSPYSMGRYTCQIQHDGQQVTHVNYQHLD"
     gene            1153..>1467
                     /locus_tag="PLY05_05835"
     CDS             1153..>1467
                     /locus_tag="PLY05_05835"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HNC02860.1"
                     /translation="MEMSVTTPAILFPAISLLMLAYTNRFLALATLIRSLHAQHEQKK
                     NDPSYRGQLANLRKRLRLIRRMQTFGGISFFFCVVSMLSLFFGNEQLGRWVFAVSLVC
                     LML"
     assembly_gap    1468..1567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1601..1882
                     /locus_tag="PLY05_05840"
     CDS             1601..1882
                     /locus_tag="PLY05_05840"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HNC02861.1"
                     /translation="MNYLSLEPFLTQHPDWRLRGTKLHREYVFKDFVQAFGFMSQVAL
                     LAERANHHPDWSNSYNRVSIDLTTHDAQSVTGKDLALAVAIEKVWLNYK"
     gene            1916..3367
                     /locus_tag="PLY05_05845"
     CDS             1916..3367
                     /locus_tag="PLY05_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004720426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC02862.1"
                     /translation="MTAMFTIQEADPQQIQNVFALQQATAIRWRESTAEQRIARLKSL
                     RDAVLAHRDAFYQAFAKDYRKPSAEVDVTELMPVLDEIRHATGRLKQWMKPEKVKPTS
                     LMLTTSAWIEYQPRGRVLIIAPWNYPLNLCFGPLVSALAAGNTAIIKPSEMMPNVSAV
                     MAKIIQETFTPQEVALFEGSLPTAEALLALPFDHIFFTGSPMVGKVVMAAAAKNLTSV
                     TLELGGKSPTIIDQSANLQVAAETLMWGKFLNNGQTCVAPDHIYVHQAVKDAFVAECR
                     KVIHARYGATAGEQKMNPDLTRIVNTRHTQRIANLLNEAVELGAKITVGGQTDIESCY
                     IAPTIVENIPTQAKIMSEEIFGPVLPIISFEHIDTVIAEINANPKPLALYIWSYNEEN
                     IEKVVSRTSSGGVCINHCLMQFVHGNLPFGGVNNSGIGNSHGHFGFKAFSHERAVVKA
                     SKLMMVRLFFPPYDESRRKVIKMTVDSMKWPML"
     gene            3602..>5427
                     /gene="tkt"
                     /locus_tag="PLY05_05850"
     CDS             3602..>5427
                     /gene="tkt"
                     /locus_tag="PLY05_05850"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004927438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNC02863.1"
                     /translation="MPAVALPQRRLANAIRALAMDAVQKANSGHPGAPMGMADIAEVL
                     WRGVFKHNPKNPNWINRDRFVLSNGHGSMLQYALLHLTGYDLSIDDLKHFRQLHSRTP
                     GHPEYGYTAGVETTTGPLGQGIANAVGFAIAEKTLAAQFNREGFDVIDHYTYTFLGDG
                     CLMEGISHEVCSLAGTLKLGKLVAFYDDNGISIDGEVEGWFTDNTAQRFAAYGWQVIG
                     PIDGHDSDAIRTAIIEAHSDTTRPSMIICKTVIGCGSPNKQGKEECHGAPLGAAEIAA
                     AREAMAWDYDAFEIPADVYEAWDCSVKGDVLEKNWQGQFDAYQKAHPDLAQELLRRLK
                     GELPASFALQAKNYIEEVALKGETIASRKASQNALNAFGPLLPELLGGSADLAGSNLT
                     LWKGCHGIQENAAGNYVFYGVREFGMSAIMNGIALHGGFIPYGATFLIFMEYARNAVR
                     MSALMKQRIIYVFTHDSIGLGEDGPTHQPIEQLASLRGTPNMSVWRPADAVESAVAWL
                     SALQKNDGPTSLIFSRQNLPHIERDALQLDNISKGAYVLAEEKGVLEAIIIATGSEID
                     LALKAQKILTEQGKGVRVVSMPSVDVFMAQDEAYHQKVLPNEV"
BASE COUNT         1490 a         1009 c         1207 g         1621 t
ORIGIN      
        1 ttgttaattg agcatgatgt taaattggtc atgggtttgt gtcatcgggt ggcggtgttg
       61 gatttcggtc aactgattgc cgaaggtgtc cctgccgatg tgcaaaaaga ccccaaagtc
      121 attgctgcct atttgggagg tagtgtatga gcttattaac cgtcaaaaac ttacaaattg
      181 cttatggtgg cattcaagcg gttaagggca ttagttttga gattaaacaa ggcgagttgg
      241 tgagcttaat tggtgctaat ggtgcaggca aaacctcaac cctaaattgt ttgggtggat
      301 tgctaaaacc cgcatcaggc gagattagtt atcaaggaca aaacttatta actattccta
      361 ctcatgatcg agttaaacat ggtttagcct tagtgcccga aggacgaggg atattttcta
      421 aattgaccgt tgccgaaaac ttacgcatgg gtgcttatca tcgtcagtta ggcaaagatg
      481 aactagagca agcctatcaa cgttttcctc gtttacatga acgcaaagag caattagcag
      541 gcacgttatc aggtggcgaa caacaaatgt tggccattgt gcgcgcttta ctgagcaaac
      601 ccaaattatt gttactcgat gagccatcaa tgggtttagc cccgattatg gttgaaaaaa
      661 ttttttctac tattagcgaa gtcaccaaag agggcgtggc ggtgttgctc gttgaacaaa
      721 acgctaaagc cgctttagcc ttggcacagc gtgcttatgt gatggaaagt ggcgtcatta
      781 gtcttgcagg ggatgccaaa gacttagccg ctgatccgcg tgtgcgagcc gcctatttgg
      841 gggagtgatg attaaaaaag tattggcaag tatgtttatg ggttatttag tctttgtgtt
      901 ggtgatgtgg tttttttctg cccagcaaat taagcaaacc tgccaacaaa taccaatgag
      961 cctgcctgtt tctaaacttg agccaatttt agattctttg tatttaacca aaatcatgcg
     1021 ccgatcatca gaacagaaaa tacaaactat tttgttgcat agcccctata gcatggggcg
     1081 ttatacttgc caaattcaac atgatggtca gcaagtgacc catgttaatt atcagcattt
     1141 ggattaaaca ttatggaaat gagtgtcacc acgcctgcaa tcttatttcc tgctatttct
     1201 ttgttaatgt tggcttatac caatcgtttt ttggccttag caaccctaat ccgtagcttg
     1261 cacgcccagc atgaacaaaa gaaaaatgat cccagttatc ggggccagtt agccaattta
     1321 cgcaagcgtt tgcgcttaat tcggcgtatg caaacctttg gtggtattag ttttttcttt
     1381 tgtgtggtct cgatgttaag tttgtttttt ggcaatgagc aactagggcg ttgggtattt
     1441 gcagttagct tggtgtgctt gatgttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnata gggatgttct agcatatagg tataataaca atgaactatc tcagtctaga
     1621 gccattttta actcaacatc ctgattggcg tttacgcggc actaaacttc atcgagagta
     1681 tgtctttaaa gattttgtcc aagcctttgg ttttatgagc caagtagctt tgttagccga
     1741 aagagcaaat caccatccag attggtcaaa tagttataac cgagtcagta tcgatttaac
     1801 cacacatgat gcgcaaagtg tcacaggtaa agatttagca ttagctgttg ccatagaaaa
     1861 agtttggcta aactacaaat aattaaattg aaaaaaattc tcagagggtt acatcatgac
     1921 cgccatgttt actatacaag aggcagaccc acagcaaata caaaatgtgt ttgccttgca
     1981 acaagcaacc gccattcgtt ggcgtgaatc aacagcagag cagcgtattg cacgtttaaa
     2041 aagcttaaga gacgccgtgt tagcgcatcg ggatgcgttt tatcaagcct ttgccaaaga
     2101 ctatcgcaag ccctctgccg aagttgacgt gactgaatta atgccagttt tagatgaaat
     2161 tcgtcatgca acaggtcgtt taaaacaatg gatgaaacct gaaaaagtta agccaaccag
     2221 tttaatgtta accaccagtg catggattga atatcagcca cgtggccgtg tattaattat
     2281 tgctccgtgg aactatccac ttaatttatg ttttggccct ttagtatcgg cattggcggc
     2341 aggcaatacc gccattatta agccttcaga aatgatgcct aatgtgtcgg cagtgatggc
     2401 aaaaattatt caagaaacct ttacccccca agaagtcgct ttatttgaag gtagtttgcc
     2461 aacggctgaa gctttattag ccttaccctt tgaccatatc ttttttacgg gttcgccaat
     2521 ggtgggcaaa gtggtgatgg ctgctgctgc gaaaaatttg accagtgtca ccttagaatt
     2581 aggtggcaaa tcacccacca ttattgacca aagtgccaat ttacaggtgg ctgctgaaac
     2641 gctaatgtgg ggtaaatttt taaataatgg tcaaacttgt gttgcacctg accatattta
     2701 tgttcatcaa gcggttaaag atgcttttgt cgcggagtgt cgtaaagtga ttcatgcgcg
     2761 ttatggtgca accgcaggcg agcaaaaaat gaatcccgat ttaactcgta ttgttaatac
     2821 tcgccatacc caacgtattg ctaatttatt aaatgaagcc gttgaacttg gcgcaaaaat
     2881 tactgtcggt ggccaaacag atattgaaag ttgttatatt gcaccaacga ttgttgaaaa
     2941 cattcctacg caagccaaaa tcatgagtga ggaaattttt ggcccagtat tacctattat
     3001 tagttttgag catattgata cggtgattgc cgaaattaat gccaatccta agcctttagc
     3061 gttatacatt tggagttata acgaagaaaa tatcgaaaag gtagtctctc gcaccagttc
     3121 gggtggggta tgtattaatc actgtttaat gcagtttgtg catggtaatt taccgtttgg
     3181 tggcgtgaat aattcgggca ttggtaattc gcatggtcat tttggtttta aagcattttc
     3241 tcacgaaaga gctgtggtta aagcgtcaaa attaatgatg gtgcgtttat tcttcccacc
     3301 ttatgacgag agtcgtcgta aagtcatcaa aatgactgtt gattcgatga aatggccaat
     3361 gttgtaaatt ttctcctgtt tgtaaggcgt ttctatttgc tctaaagcct taattaagcg
     3421 acaattacgc accttatttt taggcttgtg caattgtcgc cattaagtcg ctaaacattc
     3481 caatcaaatg cgcgtgtttt atcgctataa agccgttgat aaatatttga atgtaaacag
     3541 ctaagcgatg attgcataag tcccatagtt gattctcgac tgcccgccat tggagtcttt
     3601 tatgcccgct gttgccttac ctcaacgtcg tcttgctaat gctattcgcg cccttgccat
     3661 ggatgctgtt caaaaagcaa attcaggcca tccaggtgcg ccaatgggta tggccgatat
     3721 tgccgaagtg ttatggcgtg gtgtgtttaa acataatcct aaaaacccaa attggattaa
     3781 tcgtgaccgt tttgtgttat cgaatggtca tggttcgatg ttgcaatatg ctttattaca
     3841 tttaactggc tacgatttaa gcattgatga cttaaaacac tttcgccaat tacatagccg
     3901 cacaccgggt catcctgaat atggttatac cgcaggtgtt gaaacgacaa ctggcccatt
     3961 agggcagggt attgctaatg cagtgggttt tgcgattgcc gaaaaaacct tagcagctca
     4021 atttaaccgt gaaggttttg atgtcataga ccactacacc tatacgtttt tgggtgatgg
     4081 ttgtttaatg gaaggtattt ctcacgaagt atgttcttta gcaggcacat taaaattagg
     4141 taaattagtg gccttttatg atgacaatgg tatttcgatt gatggtgaag ttgaaggttg
     4201 gtttaccgat aataccgcgc aacgttttgc cgcttatggt tggcaagtga ttggcccaat
     4261 tgatggtcat gatagcgatg ccattcgtac tgcaattatt gaagcacata gcgataccac
     4321 acgcccaagt atgattatct gcaaaaccgt aattggttgt ggctcaccga ataaacaagg
     4381 caaagaagaa tgtcatggcg caccattagg cgctgctgaa attgctgctg ctcgtgaagc
     4441 aatggcatgg gattatgatg cttttgaaat tcctgctgat gtttatgaag cttgggactg
     4501 tagtgtaaag ggtgatgtgt tagagaaaaa ttggcaaggc caatttgatg cttaccaaaa
     4561 agctcaccca gacttagctc aagaattatt acgccgttta aaaggtgaat taccagcctc
     4621 ttttgcgtta caagctaaaa actatatcga agaagttgct ttaaaaggcg aaaccattgc
     4681 ttctcgtaaa gcctcacaaa atgctttaaa tgcctttggc ccattattac ccgagttatt
     4741 aggtggctcg gctgacttgg ctggctctaa tttaacctta tggaaaggtt gtcacggtat
     4801 tcaagaaaat gccgcaggta attatgtgtt ttatggggtg cgcgaatttg gtatgagcgc
     4861 gattatgaat ggtattgcct tacatggtgg atttattccc tatggtgcaa ccttcttaat
     4921 ttttatggaa tatgcgcgta atgcggtgcg tatgagcgcg ttaatgaagc agcgtattat
     4981 ttatgtcttt actcatgact caattggttt gggtgaagat ggcccaactc atcaaccgat
     5041 tgaacaatta gccagtttac gtggtacgcc taatatgtcg gtttggcgac ctgcggatgc
     5101 ggtagaatct gctgtggcat ggttgtctgc tttgcaaaaa aatgatggcc caacctcttt
     5161 aattttctct cgtcaaaact taccacacat tgagcgtgat gccttgcaat tagataacat
     5221 tagcaaaggt gcttatgtat tggccgaaga aaaaggtgta ttagaggcga ttattattgc
     5281 cacaggttca gagattgact tggcgttaaa agcgcaaaaa atcttaaccg aacaaggtaa
     5341 aggtgtgcgt gtggtgtcta tgccatctgt tgatgttttt atggcacaag atgaagcgta
     5401 tcaccaaaaa gtattaccta acgaagt
//
LOCUS       DAOQMB010000131         5374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_6908_length_5374_cov_14.586012, whole genome shotgun sequence.
ACCESSION   DAOQMB010000131 DAOQMB010000000
VERSION     DAOQMB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6908_length_5374_cov_14.586012"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="PLY05_05855"
     CDS             complement(<1..834)
                     /locus_tag="PLY05_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02864.1"
                     /translation="MSAIIRLTDALLNIQGIDAHKFLQGQTTTDFKEVTPEQSRLGGY
                     ANLKGRLVFSFRAIEWPAQSIHLVINQALLATAKNTLQKYIVFSKAQISTPDTSVIGV
                     LGAESEKLLTELFGFCPTTINQTLSNEQISLTRVDGDSRWLLLVKAEFSETVWQQLSQ
                     QATIASINDWRLAQIAAGETPVLAETTELYQPQELNYLPLNAISYNKGCYTGQEIIAR
                     LYFRGKLKQWTHRFTVKLSELPALNTVIYDATGHSQGHVVLAAQADAHTVELLAIVRH
                     DH"
     gene            911..1177
                     /locus_tag="PLY05_05860"
     CDS             911..1177
                     /locus_tag="PLY05_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HNC02865.1"
                     /translation="MRELTDAERAMFWRARRGLKELDVFFEPFMRDHYLLLPENEQVM
                     VAKLIKCEDPDLLAWFMGYEQPEDPDLVHIIEVFHERLGTGHSN"
     gene            1146..1562
                     /locus_tag="PLY05_05865"
     CDS             1146..1562
                     /locus_tag="PLY05_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02866.1"
                     /translation="MSVWELDTAIKPSRYQQWLNGLFYSVAMAAIWCAYLPTVAQLGL
                     TIVLLAVACLHSVKLAKTLPVVSHLSSKADDWFIELATGQRLKVKLNRYFLWRYLVVL
                     SYQSPHIRVPYVVVIFPDSIDKDNYRRLQVKLRLLS"
     gene            complement(1663..3282)
                     /gene="nadB"
                     /locus_tag="PLY05_05870"
     CDS             complement(1663..3282)
                     /gene="nadB"
                     /locus_tag="PLY05_05870"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNC02867.1"
                     /translation="MTHFDYDVLIIGSGAAGLTLALKLPKHLKIAVLSKASTSEASTY
                     YAQGGVAAVLDETDTTDSHVQDTLVAGAGLCHKDTVTFTVEHGAQAIQWLLQQGVRFT
                     LDDSEQLHLTREGGHSHRRIIHAADTTGVAISTTLVQRVHECDHIQLLENRVAVDLIT
                     TKKLGLEQEPNRVIGAYVLNQEHNRVERFRARFVALATGGASRAYFYTCNPDIATGDG
                     IAMAWRAGCRVANMEFNQFHPTSLYHPQAKSFLITEAMRGEGAILRLPDGERFMPRFD
                     SRAELAPRDIVARAIDFEMKRLGIRCVYLDITHKDPEFIISHFPNIHKTCLKYGIDIT
                     KDWIPVVPTAHFTCGGVMVNLASETDIENLYAIGETSFTGLHGANRMASNSLLECFVF
                     AMAAAQSIEQRLANTPPPPTCPEWDDSQVRHADEDVIILHNWDELRRFMWDYVGIVRT
                     DKRLERALRRIELLKVEIGEYYSNYKVSPNLLELRNLVLVSELIVRSALARKESRGLH
                     YTLDYPQMNTDLVDTVLTPPEFFLTQQEVIS"
     gene            3429..3854
                     /locus_tag="PLY05_05875"
     CDS             3429..3854
                     /locus_tag="PLY05_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HNC02868.1"
                     /translation="MLIQQAVIAKIDQQGIWVNAMRQSACGNCQQERSCQQSSDWLKS
                     SKQSALILVSPSQLAVNIGDQVEIAMPEQQLWQGLIFLYVLPLAALTLGAVIGQWYAQ
                     EMGAVLFSVCAFVVSLVIIHYTQQRQHDNCLPIISKKIT"
     gene            3943..5367
                     /locus_tag="PLY05_05880"
     CDS             3943..5367
                     /locus_tag="PLY05_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HNC02869.1"
                     /translation="MKEQQAKTVRTWLVGVICATGIQSSWAATAVMDFSDLVEKNAKA
                     VVNISASTKAKKRNAQQLQQQQVPDIFRHFFGDQFDFAPQQQNKQSFGSGFIISKEGY
                     VLTNNHVVDHADKVIVKLNDRREVEAEVIGTDERTDVALLKIKADNLPVVEIGNPDKL
                     KVGQPVLAIGSPFGFDYSATAGIVSAKSRALPNESYVPFIQTDVAINPGNSGGPLFNA
                     DGEVVGINSQIYSRSGGYMGLAFAIPIDVAMDVVDQLKDKGKVSRGYLGVVIQDIDRD
                     LAEAYGLAKPAGALVAKVLPDTPAEKSGLKEGDVIIAFNGQDIGLSSELPQMIGRAKV
                     GKKVPLTLLRDGKKMDIPFEVASLPDDEEDSPEKAAEPDLNSLGVGLRNLNDQEKTQL
                     KLEGGVLVLRLADDGAAADAGLRANDVIVRLNNQTIKDTQEFIKIAKKLPANKAVPIM
                     IYRAGQPRILALRIEGSDKKDSKK"
BASE COUNT         1622 a         1036 c         1131 g         1585 t
ORIGIN      
        1 atggtcgtga cgaacaatcg ctaatagctc aaccgtatga gcatctgctt gtgctgcaag
       61 taccacatga ccttggctat gacctgttgc atcataaatc acggtattta aagcaggtaa
      121 ttcgcttaac ttcaccgtaa aacgatgtgt ccattgtttc aatttgcctc taaaatacaa
      181 acgtgcaatg atttcttggc ctgtataaca gcctttgtta taactaatcg catttaatgg
      241 taaatagttt agttcttgag gttgataaag ttcggtcgtt tctgcaagga cgggtgtttc
      301 gcctgctgcg atttgtgcta aacgccaatc attgattgag gcaatagtcg cttgctgact
      361 gagttgttgc cagactgttt cgctaaactc tgctttaact aataataacc aacgagaatc
      421 accatccacc cgagtcagac tgatttgctc attgcttaat gtctgattaa tcgtggttgg
      481 acaaaaacca aataattcag tgagtaattt ttcactctct gcacctaaaa caccaatcac
      541 actcgtatcg ggtgtgctga tttgcgcttt agaaaaaaca atatattttt gcagggtgtt
      601 tttcgcagtg gctaataacg cttgattaat cactaaatga atactttgag caggccattc
      661 aatcgcgcga aaactaaaca ccaagcgacc ttttaaattg gcataaccgc ctaggcggga
      721 ttgctcgggt gttacttcct taaagtcggt ggtggtttgt ccttgtaaaa acttatgagc
      781 atcaatacct tgaatgttta acagagcatc ggttaaacga ataatcgcgg acataatatc
      841 ctcttatcta atcaactatt tttcagtaga aatagcatat aatactttaa tagaatcagc
      901 gagatgaaaa atgcgtgaat taacagatgc cgaacgtgcc atgttttggc gtgcaagacg
      961 tggtttaaaa gaattagatg tattttttga accgtttatg cgtgaccatt atttattatt
     1021 acctgaaaat gaacaagtca tggttgccaa attaattaaa tgtgaagacc ctgatttatt
     1081 ggcatggttt atgggttatg agcaacctga agaccccgat ttagtccata ttattgaagt
     1141 gtttcatgag cgtttgggaa ctggacacag caattaagcc atctcgttat cagcaatggc
     1201 ttaatgggtt attttacagt gtggctatgg cggctatttg gtgtgcttat ttaccaacag
     1261 tggcacaatt aggtttaacg attgtgctgc tagctgttgc ttgtttacat agtgttaaac
     1321 tcgccaaaac cttgccagtc gttagtcatt taagtagcaa agcagacgac tggtttattg
     1381 agttggcaac aggccaacgt ctaaaagtta aattaaatcg ttatttttta tggcgttatt
     1441 tggtggtgtt aagctatcaa agtccacata ttcgcgtgcc ttatgtggta gtgatctttc
     1501 ctgatagcat tgataaagat aactatcggc gtttgcaggt taagttgaga ttattatctt
     1561 aggacttaca cggttatcgc ttatttgctc gctaaataat cgttcttaac gatatttagc
     1621 ttacaaaacg tgctaattgc agcacaatgc gtaagttctg atctaactga taacctcttg
     1681 ctgagtcaga aaaaactcag gtggcgttaa cacagtatcc actaaatcag tattcatttg
     1741 tggataatct aaggtgtaat gcaaaccacg cgattctttg cgagcaaggg cagagcgaac
     1801 aattaactca ctgactaaca ctaaattgcg taactctaat aggttaggtg agactttata
     1861 attactatag tattcgccaa tttcgacttt taataattca atacgacgta aagcccgttc
     1921 taaacgttta tcagtacgaa caatacccac ataatcccac ataaaacggc gtaactcgtc
     1981 ccagttatgc aaaataatca catcttcatc ggcatgacgc acttggctgt catcccattc
     2041 agggcaagta ggcggtggcg gtgtatttgc caagcgttgc tcaatggatt gtgccgctgc
     2101 catcgcaaag acaaaacatt ctaataatga gttgcttgcc atgcgatttg caccatgtaa
     2161 acctgtaaac gaagtttcgc caatcgcata taagttttca atatcggttt cactggccaa
     2221 attaaccatc acgccgccac aggtgaagtg agcggttggc accacaggaa tccaatcctt
     2281 agtaatatca ataccatatt ttaagcaagt tttatgaata ttcggaaagt gactaatgat
     2341 aaattcgggg tctttgtggg taatgtctaa ataaacacaa cgaatgccaa gacgtttcat
     2401 ttcaaaatca atggcacgag cgacaatatc ccgtggtgca agttcagcac gactatcaaa
     2461 acgtggcata aaacgctcac catcaggtaa acgtaaaatt gcgccttcac cacgcatcgc
     2521 ttcggtaatc aaaaaagatt tagcttgtgg atgatataag ctggttggat gaaattgatt
     2581 aaattccata ttagctacac gacagccagc gcgccacgcc atcgcaatgc catcacccgt
     2641 agcaatatcg ggattacagg tataaaaata tgcacgactt gcaccacctg tggccaaagc
     2701 cacaaaacgt gctctaaaac gttcaactcg attatgctct tgattgagca cataagcacc
     2761 aatcacacga ttgggttctt gctccagccc aagttttttc gtggtaatta aatcaacggc
     2821 cactcgattt tcgagaagct gaatatgatc acattcgtgg acacgttgaa ccaaagtggt
     2881 agaaatagca accccagtgg tatcggcggc atgaataata cgacgatgag aatgcccccc
     2941 ttcacgggtt aaatgtagtt gctcactatc atctaaggta aagcgaaccc cttgttgcaa
     3001 cagccattga atcgcttgtg caccatgctc tactgtaaat gtgacggtat ctttatgaca
     3061 caatccagca cctgcgacca aagtatcttg tacatgactg tctgtggtat ctgtttcatc
     3121 taatactgcg gcaacaccac cttgtgcata gtaggtactg gcttcgctgg ttgaagcttt
     3181 ggataaaacg gcaattttta aatgctttgg caattttaac gccaacgtta agcctgctgc
     3241 accactacca ataatcagta cgtcataatc aaagtgtgtc atagtgtatt cgtcaaactg
     3301 ccattatgcg gtcataaaga gaagatatag ggcagagtat aaagcagagt gattttgtac
     3361 aatagtacaa atagtctatt attggacagc aatagtacaa ataacagtgc tttttgatcg
     3421 gattatctat gttaatacaa caagcggtga ttgctaaaat tgatcagcag gggatttggg
     3481 tcaacgctat gcggcaaagt gcgtgtggta actgccagca agagcgatcc tgtcagcaaa
     3541 gctctgattg gctaaaaagc tcaaaacaat cagcattaat tttagtctca ccgagtcaat
     3601 tagccgtgaa tattggtgat caagttgaaa tagctatgcc agaacaacaa ttgtggcaag
     3661 gattaatatt tttatatgtt ttacctttag cggcattgac cttgggggca gtgatcggtc
     3721 aatggtacgc tcaagaaatg ggggcagtgt tgttttcggt ttgtgctttt gttgtgtctc
     3781 tagtcatcat tcactatacc cagcaacgac aacacgacaa ttgtttacca attattagca
     3841 aaaaaataac atagcttgaa aaaacttaca cagcccctat agcgataatc ataaaagctt
     3901 tttatctttt agggcattca acttgcagtc aggatttaca atatgaaaga acaacaagca
     3961 aaaacagtgc gtacttggtt ggttggggtt atttgtgcca cagggataca aagttcatgg
     4021 gccgcaacgg cggtgatgga tttttctgat ctcgtggaaa aaaatgcgaa agccgtggtt
     4081 aatattagtg catcaaccaa agccaaaaaa cgtaatgcac aacagcttca acagcaacaa
     4141 gttcccgata tttttagaca tttttttggt gatcaatttg attttgcacc tcaacaacaa
     4201 aataaacaat catttggctc aggttttatt atttctaaag aaggttatgt tttaaccaat
     4261 aatcatgtgg ttgatcatgc cgataaagtg attgtcaaat tgaatgaccg ccgtgaagta
     4321 gaagctgaag tcattggtac agatgaacgt accgatgtgg cgctattaaa aattaaagca
     4381 gacaatctgc ctgtcgttga aattggcaat cccgataaat taaaagtggg tcaacctgtg
     4441 ttagccattg gctctccgtt tggttttgat tattcagcaa cagcgggtat tgtcagtgct
     4501 aagtctcgtg ctttgcctaa tgagtcttat gtccctttta ttcaaactga cgtagccatt
     4561 aaccctggta attcgggtgg ccccttattt aatgctgatg gtgaagtagt tgggattaac
     4621 tcacaaattt atagccgttc tggcggttat atgggcttag cctttgcgat tccgattgat
     4681 gtggcaatgg atgtggttga tcaactaaaa gacaaaggta aagtgtcgcg tggttatttg
     4741 ggggtagtga ttcaggatat tgatcgtgac ttagcggaag cgtacggctt agctaaacca
     4801 gcaggtgctt tggtcgccaa agtattgcct gatacccctg cggaaaaatc aggtttaaaa
     4861 gaaggcgacg tgattattgc ttttaatggc caagatattg gtttgtcttc tgaattaccc
     4921 caaatgattg gtcgtgctaa ggttggtaaa aaagtgccat taactttatt acgtgatggt
     4981 aaaaaaatgg atattccttt tgaagtcgcc tctttacccg atgacgaaga agattccccc
     5041 gaaaaagcag cagaacctga cttaaatagt ttaggtgtgg gtttacgtaa cttaaatgac
     5101 caagagaaaa ctcaactaaa attagagggt ggtgtgttgg tgttacgttt agcagatgat
     5161 ggtgcggcag ctgatgcagg tttacgggcg aacgatgtga ttgtacgttt aaataatcaa
     5221 accattaaag acacacaaga gtttataaaa attgccaaaa aactccctgc caataaagca
     5281 gtgccaatta tgatttatcg tgcaggtcag ccacgtattt tggctttacg tattgaagga
     5341 tcagataaaa aagacagtaa aaagtaattt ttac
//
LOCUS       DAOQMB010000132         5285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7100_length_5285_cov_17.187189, whole genome shotgun sequence.
ACCESSION   DAOQMB010000132 DAOQMB010000000
VERSION     DAOQMB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_5285_cov_17.187189"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /gene="tsaB"
                     /locus_tag="PLY05_05885"
     CDS             <1..684
                     /gene="tsaB"
                     /locus_tag="PLY05_05885"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNC02870.1"
                     /translation="IMPILALDTASEACSVAIHLDNGEIISRYEYAPRLQTQLLLPMV
                     DELLAQCQLSPQALTAVAYSRGPGSFTGVRISAAAAQGLAFGWDLPVLAISSLQTLAQ
                     GAYRVLQQEQVISVFDARMNEIYLGAYQLSGQLMQPVIEEVVCSPKQIPNIPEQDWAL
                     VGSGTIYADEIKQQTSIQQVFLDIHPQAYDVAVLAQQAWQQGLAQAPELALPVYLRDS
                     VWQKLPNKS"
     gene            696..1523
                     /locus_tag="PLY05_05890"
     CDS             696..1523
                     /locus_tag="PLY05_05890"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009512322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNC02871.1"
                     /translation="MDIILLLKAVLMGLIEGATEFLPVSSTGHLIVTASLINFWTPEK
                     RHVFEVAIQLGAILAVCYEYRQRLINVTQGLFARQQQAWIFASNILIAFLPAAIIGLF
                     ARDFIKGYLFNPVSVATALVIGGFVILYAENRQHKITVPEVDDMKPLDALKLGFAQCL
                     ALIPGTSRSGATIIGGLFFGLSRKAAAEFSFFLAIPTMVAATAYDVYKSRDDFIMADF
                     PVFAVGFIAAFIAGLLVVRTLVKYVANHDFKIFAYYRIVFGCFILATAWFGLIDWSH"
     gene            1523..2299
                     /locus_tag="PLY05_05895"
     CDS             1523..2299
                     /locus_tag="PLY05_05895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNC02872.1"
                     /translation="MFILYLADYQQQAQQLALTLGVEAFLLANTERKTILAWQKQGSL
                     AILLAGQVALQPLSKPLPNPVMVDWANKSLLWRLQHGGGRGELLAKACGIKKDYLPKV
                     IDATAGFGRDSLLLASLGCQVQLIERSPLVAAMLFDGWQRAAQEPFLQEILPRMSLHH
                     GHAVEFLQDLSAIDYPDVVYLDPMFPDRNNSAKVKQDMQSFHVAVGADDDADALLAPA
                     LAVAKKRVVVKRPRHAQPLAEQKPSLVYEGESSRFDVYLI"
     gene            2339..>2688
                     /gene="greB"
                     /locus_tag="PLY05_05900"
     CDS             2339..>2688
                     /gene="greB"
                     /locus_tag="PLY05_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015414.2"
                     /note="necessary for efficient RNA polymerase
                     transcription elongation past template-encoded arresting
                     sites; arresting sites in DNA have the property of
                     trapping a certain fraction of elongating RNA polymerases
                     that pass through, resulting in locked ternary complexes.
                     Cleavage of the nascent transcript by cleavage factors
                     such as GreA or GreB allows the resumption of elongation
                     from the new 3'terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HNC02873.1"
                     /translation="MSYYRSPLPPKSNLITPEGYKNLQDELDYLWRVKRPEVTRSVSE
                     AAAQGDRSENAEYIYGKKLLREIDRRVRFLQKRIPELKVVSEKPKDRQRIFFGAWVTL
                     EDVDSEERLSFRIVG"
     assembly_gap    2689..2788
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2796..3896
                     /gene="glnL"
                     /locus_tag="PLY05_05905"
     CDS             2796..3896
                     /gene="glnL"
                     /locus_tag="PLY05_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HNC02874.1"
                     /translation="MVLNPPSYKLLLEHLTTAVLLVDEHLCLTYLNPSAEMLLAVSRS
                     RAVGQPLAQIFFDTDGNSTLHKMANTLKTAHPFTKREARLRVAGIQEVVVDYTVASLL
                     TPNQPPELLIEIQQIDRLLRISREESILSNHQVTKQLVRGVAHEIKNPLGGIRGAAQL
                     LARALPDNNLKEYTQIIIEEADRLRNLADRMLGPRKLPDLQSINIHECLERVRSLILV
                     EAEGEVSIVRDYDLSLPELTADPDQLIRAILNITGNALQALRENVKQQLPPMITLKTR
                     AQRQFTIGSVRHRLVLRVDIIDNGPGIPAHLIDTIFYPMVTGRASGSGLGLAIAQDIV
                     HLYHGLIECESRVGQTVFSIFLPLENGHASKR"
     gene            3880..>5285
                     /gene="glnG"
                     /locus_tag="PLY05_05910"
     CDS             3880..>5285
                     /gene="glnG"
                     /locus_tag="PLY05_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HNC02875.1"
                     /translation="MPANDKVWIVDDDRSIRWVLEKALMQEGLEATAFDDAQVLLSKL
                     QKEQPHAIVTDIRMPNIDGLTLLAKIRVDYPDIPVIVMTAHSDLDSAVSSYQGGAFEY
                     LPKPFDVDEAINLVKRAIAHHHEQFQNTLPALSQTKGVDIIGESLAMQEVFRAIGRLS
                     HSHITVLINGESGTGKELVAHALHRHSPRTSRPFIALNMAAIPKDLMESELFGHEKGA
                     FTGANQLRQGRFEQANHGTLFLDEIGDMPLDTQTRLLRVLADGEFYRVGGHIPVKVDV
                     RIIAATHQNLERLVAEGKFREDLYHRLNVIRVHIPRLRDRREDIPMLAQHFLARAAKE
                     LNVEPKVLKLETATFLQQLPWPGNVRQLENTCRWLTVMASGREILPTDLPPELQQISS
                     HEVVRVAQSWEQALAEWARKELSSGTKMLLEQALPTFEKIMITAALNYTGGRRRDAAE
                     VLGWGRNTLTRKIKELKM"
BASE COUNT         1439 a          918 c         1162 g         1666 t
ORIGIN      
        1 attatgccta tattagccct tgataccgcc agtgaagctt gttctgtcgc gattcattta
       61 gataatggcg aaattatcag ccgttatgag tatgcgccgc gcttacaaac acaattatta
      121 ttaccgatgg ttgatgagtt attagcacaa tgtcaattat cgccccaagc tttaacggca
      181 gttgcttata gtcgtgggcc gggttcgttt acaggggtgc gtatttcggc agccgcagca
      241 caaggtttag cctttggttg ggatttacct gtgttagcca tttccagttt gcaaacctta
      301 gcgcaaggag cttatcgtgt tttacagcaa gaacaagtga tcagtgtttt tgatgcgcgt
      361 atgaatgaaa tttatttagg ggcttatcag ttaagtggcc agttaatgca gcctgttatt
      421 gaagaagtgg tctgctcacc taaacaaata cccaatatac ccgaacaaga ttgggcattg
      481 gtgggtagtg gtacgattta tgctgatgag ataaaacagc agacatctat acagcaagtt
      541 tttttagata ttcatccaca agcttatgat gtggccgtat tggctcaaca agcatggcaa
      601 caaggtttag ctcaagcacc agagttagcc ttaccagttt atttgcgtga tagtgtgtgg
      661 caaaaattgc ctaataaatc ctaagagagt attagatgga tattatttta ttattaaaag
      721 ccgtattaat gggtttgatt gaaggggcaa cagaattttt gccagtctct agtacaggcc
      781 atttaattgt taccgcgtca ttaattaatt tttggacacc cgaaaaacgc catgtttttg
      841 aagtggcgat tcagcttggt gcaattttgg cagtatgtta tgaatatcgt cagcgtttaa
      901 ttaatgtcac tcagggttta tttgcaaggc aacaacaagc ttggatattt gccagtaata
      961 ttttaattgc ttttttacca gcagcaatta ttggtttgtt tgcccgtgat tttattaaag
     1021 gttatttgtt taaccctgtt tcggtggcga cggccttggt gattggtggt tttgtcattt
     1081 tgtatgccga aaatcgtcaa cacaaaatta ccgtaccgga agtagatgat atgaaacctt
     1141 tagatgcgct aaaactaggt tttgcccagt gtttagcctt aattcccggt acatctcgtt
     1201 ctggtgcaac aattattggt ggcttgtttt ttggtttgtc tcgtaaagca gcggctgagt
     1261 tttccttctt tttagccatt ccaacgatgg ttgctgccac ggcttatgat gtctataaaa
     1321 gccgtgatga ttttattatg gcggactttc ctgtatttgc cgttggtttt atagctgcat
     1381 ttattgcagg tttattggta gtgcgtacct tagtcaagta tgttgccaac catgacttta
     1441 aaatattcgc ttattatcga attgtttttg gttgttttat tttagcaacc gcatggtttg
     1501 gtttgattga ctggtcgcat tgatgtttat tttgtattta gctgactatc aacaacaagc
     1561 tcaacaatta gccttaacac tcggtgttga ggctttttta ttggctaata ccgagcgtaa
     1621 gactatcttg gcatggcaaa agcagggcag cttggccatt ttattggcag ggcaagttgc
     1681 cttacaaccg ttaagtaaac ctttgcctaa tccagtcatg gttgattggg caaacaaatc
     1741 tttgttgtgg cgtttacaac atggtggtgg tcgaggcgag ttattggcta aagcatgtgg
     1801 tattaaaaaa gattatcttc ccaaagtgat tgatgccaca gcaggctttg gtcgagatag
     1861 tttgttgttg gcgtcattag gttgccaagt acaattgatt gaacgctcgc cattggtagc
     1921 agctatgctg tttgatggtt ggcaacgcgc agcacaagag ccttttttac aagaaatttt
     1981 gcctagaatg tctttgcatc atggtcatgc agttgaattt ttacaagatt tatcagccat
     2041 tgattatcct gatgttgttt atttagaccc catgtttcct gatcgaaata atagtgctaa
     2101 agtcaaacaa gatatgcaaa gctttcatgt cgctgttggt gcagatgatg atgccgatgc
     2161 gttattagct ccagccttag ccgttgccaa aaaaagagtt gtggttaaac ggccacgcca
     2221 tgcgcagcct ttagcagaac aaaaaccttc tttagtgtat gaaggtgaaa gcagtcgttt
     2281 tgatgtttat ttaatctgat ctaaaacatt aatttatttt tttaatatat tgattattat
     2341 gagttattat cgttctccat taccacccaa aagtaacctg attacgcctg aaggttataa
     2401 aaacttacaa gatgaacttg attatttgtg gcgagttaag cgtcctgaag tcacacgttc
     2461 tgtgagtgag gctgctgcac aaggtgatcg ttccgaaaat gccgaatata tttatggtaa
     2521 aaaattactg cgtgaaattg atagacgagt caggtttttg caaaaacgca ttcctgagct
     2581 aaaagttgtc agtgaaaaac caaaagaccg ccaacgaata ttttttggag cgtgggttac
     2641 attagaggat gttgacagcg aagaacgatt atcctttaga attgtgggnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnga ggaatatagt gttgaatcca cctagctaca
     2821 aactcttgtt agagcattta accaccgcag tgctgttagt ggatgagcat ttatgtctaa
     2881 cttatcttaa tccatcggct gaaatgttat tggccgttag tcgaagtcga gcagtaggtc
     2941 aaccattggc gcaaatattt tttgatactg atggcaacag tacattgcat aaaatggcaa
     3001 atactctcaa aactgcccat ccttttacta aacgtgaagc tcgtttgcgt gtggcaggta
     3061 ttcaagaagt ggtagtcgat tatacggtgg catcattatt aacgccgaat cagccgccag
     3121 aactattaat tgaaattcaa caaattgatc gtttattacg tatttcacgt gaagaatcta
     3181 ttttgtccaa tcatcaagtg actaagcaat tagtgcgtgg tgttgcccat gaaattaaaa
     3241 atccattagg tggaattcgt ggtgcggctc agttattggc aagagcatta cctgacaata
     3301 atcttaaaga atatacccaa attattattg aagaagctga tcgtttgcga aatttggctg
     3361 atcgaatgtt ggggccacgc aaattaccag atttacaatc tattaatatt catgaatgct
     3421 tggaacgtgt tcgctcttta attttggttg aagccgaagg cgaggtaagt attgtccgcg
     3481 attatgactt atctttgcca gagttaactg ctgatccaga tcaactcatt cgagctattt
     3541 tgaatattac aggtaatgcc ttacaagcat tacgcgaaaa tgttaagcaa caattgccgc
     3601 caatgatcac cttaaaaaca cgcgcacaaa gacagtttac cattggttcg gtgcgtcatc
     3661 gtttagtgtt gcgagtcgat attattgata atgggcctgg tattcctgca catttaattg
     3721 atacgatttt ttatccaatg gtgacagggc gtgcgagtgg ttcagggttg ggtttggcaa
     3781 ttgctcaaga tattgttcat ttatatcacg gtttgattga gtgtgaatct cgggttgggc
     3841 aaacagtgtt tagtattttt ttacctttgg agaatggcca tgccagcaaa cgataaagta
     3901 tggatagttg atgatgatcg ttctatacgt tgggtgttag aaaaagcatt aatgcaagag
     3961 ggcttagagg caactgcctt tgatgatgct caagtgttgt taagtaaatt gcaaaaagag
     4021 cagcctcatg ccattgtcac cgatattaga atgccaaata ttgatgggtt aacattatta
     4081 gctaaaattc gggtcgatta tcctgatatt cctgtcattg ttatgactgc tcattctgat
     4141 ttagattcgg cggtttcatc ttatcaaggc ggtgcatttg aatatttacc caaaccattt
     4201 gatgttgatg aagcaataaa tttagttaaa cgagccattg cgcatcatca cgagcagttt
     4261 caaaacacct tgcctgcact tagtcaaacc aaaggtgtag atattattgg tgagtctcta
     4321 gcgatgcagg aagtttttcg tgctattggt aggctatcac actcgcatat tactgttttg
     4381 attaatggtg aatctggtac aggtaaagaa ttggttgcac acgctttgca tcgccattca
     4441 ccgcgcacta gtcgcccatt tattgcctta aacatggcag caattcctaa agatttaatg
     4501 gaatcagaat tatttggtca tgaaaaaggt gcatttacgg gagccaatca attacgtcaa
     4561 ggtcgttttg agcaagctaa tcatggcaca ttatttttag atgaaatagg tgatatgcct
     4621 ttagataccc aaactcgctt attaagagta ttagccgatg gtgagtttta tcgagtaggc
     4681 gggcatatac ctgttaaagt tgatgtcagg attattgccg caactcatca aaacttagag
     4741 cgtttagtgg ctgagggtaa gtttagggaa gatttatatc atcgtttaaa tgtgattcgt
     4801 gtacatattc ctcgtttacg cgatcgtcgt gaagatattc ccatgctggc acagcatttt
     4861 ttggctcgag cagcaaaaga gttaaatgtg gagccaaaag ttttaaaatt agaaacggct
     4921 actttcttac aacagctgcc gtggcctggt aacgtccgtc aactagagaa tacttgtcga
     4981 tggttaaccg tgatggcctc tggccgagag attttgccaa cagatttgcc accagaatta
     5041 caacaaatta gcagtcatga agtggtgaga gttgctcagt cttgggagca agcattagct
     5101 gagtgggcgc gtaaagagct ttcttctggc acaaaaatgc tgttagagca agccttacct
     5161 acctttgaaa aaattatgat tactgccgct ttaaactata caggtggtcg tcgtcgtgat
     5221 gctgccgaag ttttaggttg ggggcgaaat accttaactc gcaaaattaa agagttaaaa
     5281 atgga
//
LOCUS       DAOQMB010000133         5258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7142_length_5258_cov_10.579858, whole genome shotgun sequence.
ACCESSION   DAOQMB010000133 DAOQMB010000000
VERSION     DAOQMB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_5258_cov_10.579858"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..816
                     /locus_tag="PLY05_05915"
     CDS             292..816
                     /locus_tag="PLY05_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02876.1"
                     /translation="MTKSERLLYVFASSVKAGGGIHHASELAFMLGEAHTPTFTKFLA
                     DCVKKGIIRRVAQGLFESLLTPPEPTTALYKIIKKLRGNVLTYISLESQLSHTGDISQ
                     VIMGRVTVVTKGRSGTFFTPYGVIEFTHTKKPVKDIAPNLYFDTDINMYRATAQQALA
                     DLKHCNRNLHMLEG"
     gene            818..1708
                     /locus_tag="PLY05_05920"
     CDS             818..1708
                     /locus_tag="PLY05_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC02877.1"
                     /translation="MLKQQIQQIVAENPAYAAITPVIEKEILHHDILAVMVKQGAMQQ
                     LTFIGGTSLRMCYNSARLSEDLDFNGGHDFKPSHFNGLEADIQQYLQHKYETTVWVNK
                     PNQHKQGNTSSWTISIIKEATRPDLPQQKIHIDVCAIPSFDIQKRPLLNHYNIMVSTE
                     GILVPVQSLEEILVDKLIALAYRARRIKPRDIWDIVWIKQRGVVLSEALFEQKLAARN
                     KQKQEFIELLKVQLEKLRQDDEVHDDFNKEMSRFIPQQIKERTIDNPDYWFYVQTEVC
                     DVANSLINKSLVKNKFDMGL"
     gene            complement(1941..4529)
                     /locus_tag="PLY05_05925"
     CDS             complement(1941..4529)
                     /locus_tag="PLY05_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02878.1"
                     /translation="MANRHDVIIWDADVYGAPDGFSEYTYEKAYALIKQPAEPSKKLL
                     AFARDVEKLSQSNDIPPVVAQYLVGFEDDIKAEGMAAYCFNLPEYNWQSIVKILLKEA
                     IKHGLALFDEQIILVLLPNGTILPENSRKSWLQILNAPKQKNAFPKTLIELSQLFKAD
                     IGELLANHDFVLDAESSEYDEIGFSYVRQTSSVNHKIGFGYAGGNGQFELGYSIQLAE
                     NTMIEIGHKSDFQYYGDGGILLNIPSLIMNKSRFSIDSWESYEQFKTILKRSALTWSD
                     TALDIKGLDALLNGNVDNRVKQEVHQFTYMPYALIVARLADNPYFEELAVSLGRWGVN
                     SNTSWPAGVKTPLEIAWPKLVNYLRDEVKPLVSNKNKSVLVVTDSDLILETVETNHQE
                     IEQNTFNIFDVINTDELEKNFPKTLTTFNDLISRAIGSLLKSYGFIFNSNSYCDNRYS
                     IRHLKLYDDGRSLHAINITIKEDDKNYFHVDAHLEIHNRVMESIINYYSDFDFKLWQE
                     GNRVDNWQAGGLVLELARILYPQQNSLIILDKQSLINFLSILEQSVLLWSNAALNIKG
                     IDALLNGDIDVNVKKFIQQFVYMPYALIAARLANNPLFEDLAINLAPTEHDSKIWRGK
                     GKIDPFVAWPKLVEFLREEEKTIASLINTKLTTPQKTKELPPAIKESLNQYLQEHTAL
                     DWPREYTFTFWLIQDDEALFDLLMELMNCMVFKILDKAEEAWQEANNPDGLEEDETAI
                     ALREKAELAKFPPIYAAIYRIMESIYSCHSPYEMYFNLLAWGKDGVAQLTEGLNLLGL
                     TQLADAYTTVNNSIPPYDPDEDLDKKICAQQFLSAFESVMEYDNTQQFADIAEAIRKN
                     YHLFLV"
     gene            4676..5095
                     /locus_tag="PLY05_05930"
     CDS             4676..5095
                     /locus_tag="PLY05_05930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNC02879.1"
                     /translation="MHPTTPEQITQWIQDSVGNAKDDGFRVMALRPLEADCILPYQQN
                     QLRAGGTLSGPTMMTLADAAMYAAVLAHYGKQLLAVTQDFSIHFLAKPSQHDLQAQAK
                     IIKAGKRTVVLTVHIYSQDVLVALATGTYALPTQALK"
BASE COUNT         1694 a         1004 c          943 g         1617 t
ORIGIN      
        1 cgcgtaagcc taaaggcagc caattatata aaccagtgcc cactttgcga attaaacccg
       61 cacgtaacat cagttggtgg gagataacaa cggcatcggc aggagtttca cgaagcgtag
      121 caagcaaaaa acgactagcg cgcataataa atttacatcc aagaaaacaa aatgatgccg
      181 attttagaac aactccatta aaattgccgc ttatttttga ccctttgctt gtagatagtt
      241 ggccacaggt ttacacttta taacgtatta agttattttg tgtaaatcta aatgacaaaa
      301 tctgaaagat tactttacgt ttttgcaagc tcagttaagg caggaggcgg cattcatcat
      361 gcgtcagaac ttgcgtttat gttaggtgaa gcccatacac caacctttac caaatttctt
      421 gctgactgtg ttaaaaaggg cattatcaga agagtagcgc aaggtctgtt tgaaagccta
      481 ttaaccccac ccgagccaac aacagccctt tataaaatta ttaaaaaact acgtggtaat
      541 gtcttaactt atattagttt agaaagccaa cttagccata caggtgatat ttctcaagtc
      601 attatgggta gagtaaccgt tgtcacgaag ggcagaagtg gtactttttt tacgccttat
      661 ggagtaatag aatttaccca taccaaaaag ccagtcaaag atattgcgcc aaatttgtat
      721 tttgataccg atataaatat gtatcgagca acagcccaac aagcactcgc tgaccttaaa
      781 cattgcaatc gtaatcttca tatgctggag ggctaagatg ttaaaacaac agattcaaca
      841 aattgtggca gaaaatccag catatgccgc cattacacct gtcatcgaaa aagaaatttt
      901 gcatcacgat attttagccg tcatggttaa acaaggtgct atgcaacaac tcacttttat
      961 tggtggtact tcgttgcgca tgtgctacaa cagtgcgcgc ttatccgaag accttgattt
     1021 taatggcgga catgatttta agccttctca ctttaatggc ctagaggctg acattcagca
     1081 atatcttcaa cataaatacg aaacgacagt atgggttaat aaacctaatc aacataaaca
     1141 aggtaatacg tcttcatgga caattagtat tatcaaagag gctacacgac cagatttacc
     1201 acagcaaaaa attcatattg atgtttgtgc gattccctct tttgatatac aaaaaaggcc
     1261 attattgaat cattacaata tcatggtctc aacagaaggg attcttgtgc ctgtacaatc
     1321 ccttgaagaa atattggttg ataagttgat agcattggct tatcgcgcac gacgaattaa
     1381 accccgtgat atatgggata ttgtttggat taaacagcgt ggcgtagttt tatcagaagc
     1441 actttttgag caaaaattag cggcaagaaa caagcaaaaa caagagttta ttgagttgct
     1501 caaagtgcag cttgaaaagc tcaggcaaga tgatgaagta cacgacgact ttaacaaaga
     1561 gatgagccgc tttattcctc aacagataaa agagcgaacg attgataatc ctgattattg
     1621 gttttatgta caaacagaag tttgtgatgt ggcaaatagt ttaatcaata aaagcttagt
     1681 aaaaaacaaa tttgatatgg gcttgtgatt tttactcgct caggacttac gcattttgcc
     1741 aaaattagcg cgttttgtga gcataaaatc gttgaaatgc gaacaaataa gcgataactg
     1801 tgtaagtcct aaactaagcc gctcaaataa aaacagccgc aaagcaaaca aaacaatgcc
     1861 gattttagaa caactccatt aaaattgccg cattattttt tggaaaacta agatgccctc
     1921 gccattggac gagggtaaga ttaaactaaa aataagtgat aattcttacg aatagcttcg
     1981 gcaatatcag caaactgttg tgtgttatca tattccatca cactttcaaa ggctgacaga
     2041 aattgttgag cgcaaatctt tttatctaag tcttcatcag gatcatatgg cggaatactg
     2101 ttattgacgg ttgtatacgc atcagcaagt tgagttaaac ccagtagatt caagccttct
     2161 gtaagttgtg ctacaccgtc ttttccccat gctaaaagat taaaatacat ttcatatggg
     2221 ctatgacaac tataaattga ctccataatc ctataaatag ctgcgtaaat aggtggaaat
     2281 ttagcaagct ctgctttttc tcgtaaagcg atagcagttt catcttcctc taaaccatcg
     2341 gggttattgg cttcttgcca tgcttcttcg gccttatcca atattttaaa caccatacaa
     2401 ttcatgagtt ccattagaag gtcaaataat gcctcatcat cttgaattaa ccagaatgta
     2461 aaggtgtatt cacgaggcca atctagggct gtatgttctt gtaagtattg atttaagctt
     2521 tctttaatag ctggtggaag ctctttagtt ttttgtggag tggtaagttt ggtattgatg
     2581 agagaagcta ttgttttttc ttcttcacga agaaactcaa ccaattttgg ccatgccaca
     2641 aatgggtcaa tttttccttt tccacgccaa attttagaat catgttcggt gggggctaaa
     2701 ttaatcgcta gatcttcaaa tagtgggtta ttggctaaac gtgctgcaat cagtgcataa
     2761 ggcatataaa caaattgctg gataaatttt ttgacgttga catcaatatc accatttagt
     2821 aaggcatcaa tacctttaat attgagtgcc gcatttgacc ataataaaac tgattgttcg
     2881 agtattgata aaaagttgat aaggctctgt ttatcaagaa taattaggct attttgttga
     2941 ggatataaga ttcttgcaag ttctaaaacc aaaccgcctg cttgccagtt gtcaacgcgg
     3001 ttaccttctt gccataactt aaaatcaaaa tcggagtagt agttaataat actttccatg
     3061 actctattgt gaatttctaa gtgcgcatca acatgaaaat aatttttatc atcttcttta
     3121 attgtgatgt ttatcgcatg tagtgatctc ccatcatcat aaagttttag atgcctgatg
     3181 gaatatctat tgtcacaata agaattactg ttaaatatga acccataact ttttaacagt
     3241 gagccaatgg ctctgctaat aagatcattg aaagttgtca gcgtttttgg gaaatttttt
     3301 tctaattcat cagtgttgat gacatcgaaa atattaaatg tattttgttc tatttcctga
     3361 tgattcgttt caacggtttc taagattaag tcagaatctg tgactactaa aactgattta
     3421 tttttattgc taacaagcgg tttgacttca tcgcgaagat aattaaccaa ttttggccat
     3481 gcaatttcga gaggagtttt tacccctgct ggccatgaag tattactatt aactccccac
     3541 cgtcctaaac taaccgccaa ttcctcaaaa taaggattat ctgctaatcg agcaacaatc
     3601 aacgcatagg gcatataggt aaattggtga acttcctgtt taactcggtt atccacatta
     3661 ccattaagta gtgcatccag tcctttaata tctaatgcgg tatctgacca tgttaaagcc
     3721 gaacgcttaa gaatagtttt aaactgttca taactctccc aactatcaat agaaaaccga
     3781 cttttgttca taataaggct aggaatattt aataaaatac cgccatcacc atagtactga
     3841 aaatcagatt tatggcctat ttcaatcata gtgttttctg ctagttgaat tgaatatcct
     3901 aattcaaatt gaccatttcc ccctgcataa ccaaagccaa ttttatgatt tacagatgat
     3961 gtttggcgta cataactaaa accaatttca tcatattcac tcgattctgc gtctaacaca
     4021 aaatcatgat tagccaataa ttctccaata tcagctttaa aaagctgaga aagctcaatc
     4081 aaggttttag gaaacgcatt tttttgtttg ggggcattta aaatctgtaa ccacgattta
     4141 cggctatttt caggcagaat agtgccatta ggtaataaaa ctaagatgat ttgctcatca
     4201 aatagggcta aaccatgttt aatggcttct tttaataaaa ttttgacaat gctttgccag
     4261 ttatattcag gcagattaaa acagtaagct gccatacctt ctgcttttat atcatcttca
     4321 aagccaacaa gatattgggc aactactggc ggtatatcat tggattgact aagcttttct
     4381 acatcacgcg caaaagcaag taatttttta ctcggttcgg ctggttgttt tatcaaagca
     4441 taggcttttt cgtaagtata ttcgctaaaa ccatcaggtg cgccataaac atccgcatcc
     4501 caaataatga catcatgacg attggccact tttaactcct gttagcatta ttatttattg
     4561 gctagtattg tttaagcata atggctaata aaacagccgc aaagcaaata aaacaatgcc
     4621 gattttagga taactacgtt aaaattgccg cttattttct agtgacaatc aatttatgca
     4681 cccgacaacg cccgaacaaa ttacccagtg gattcaagat agtgttggca atgccaaaga
     4741 tgatggtttt cgtgtgatgg ccttgcgccc tttagaagca gactgtattt taccctatca
     4801 acaaaatcaa ttgcgtgcag gtggaactct ctcagggcca accatgatga cccttgctga
     4861 tgcagccatg tatgcagcgg tgttggcaca ttatggcaaa caacttttag cagtgaccca
     4921 agattttagt atccattttt tagcaaaacc tagccaacat gatttgcagg cacaggccaa
     4981 aattataaaa gcaggcaaac gtactgtggt tttaacagta cacatttata gtcaagatgt
     5041 tttagtggcg ttggcaacag gcacttatgc tttacccaca caagccctta agtaaataaa
     5101 attaaactag gaagatttat gaccgttcaa cctttagctg ttagtcaatt atataaatct
     5161 gcacatgcag aactccctgt taatactcgc cgtctaaaaa cctttaatga ttttttaggc
     5221 caagatcgtg ccaaagaagc agtacacatg gctttagc
//
LOCUS       DAOQMB010000134         5230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7209_length_5230_cov_16.048696, whole genome shotgun sequence.
ACCESSION   DAOQMB010000134 DAOQMB010000000
VERSION     DAOQMB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_5230_cov_16.048696"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLY05_05935"
     CDS             complement(<1..255)
                     /locus_tag="PLY05_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC02880.1"
                     /translation="MGDKSNGTRNLLFLAGPVLDILAKGLTLVIDELDTNLHTLLVQA
                     LVRLFHRPDINHHGAQLIFTTHDTSLLDAYSQFRRDQVWFM"
     gene            389..1399
                     /locus_tag="PLY05_05940"
     CDS             389..1399
                     /locus_tag="PLY05_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNC02881.1"
                     /translation="MPKANKENAIYRQWAILKILSSQRRLETQQIHQLLLEQHEIAVH
                     ENTVLRDLKNLEKIGFPLNRSDTKPIDWWLKKEWQEKIAGMTEGEALLAMLVKEYLKE
                     ILPINTLKQLDDLFELAEKKLNSQQNNHASQWLAKIRVIAAQQPQLPPVVRDSVKAVI
                     SDALMHNQVIKAQYKSHIFLLSPLALLMRGQVIYLAAVEGDDVTQVKHFALHRFDSAS
                     IAYGEKFNSPENFNIDELLKAGWGHFQQADQEKNIRLVFWCDRYLKDHLAEMRLAANQ
                     WIDFTNPQDDHYMVTVKLPYTWQLKQWLLSQGSKVRVVKPIWLREALQEEIQAMLNNY
                     KS"
     gene            1547..2233
                     /locus_tag="PLY05_05945"
     CDS             1547..2233
                     /locus_tag="PLY05_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02882.1"
                     /translation="MLELDLENLDVSFDDEFLKNEKLTWLPWVGVNFQTTTVRTLVLG
                     ESTYNWNPSSQTVQDRIKKNNHLRLVHQNNAIKFKAKSEYTRNFERAVFNNKKVTPLM
                     AKQLWHSVVYHNLVLRCMKDVKARPKYDDYLVGWPETFKLFELLSVEQCICYGLEDKK
                     IKAFKEAATLCGYEFKVQKFSKIGKYTPKIITLNINNKLIKIAFIRHPSSFFSWKNWA
                     VFMKNTPIFV"
     gene            2298..2933
                     /locus_tag="PLY05_05950"
     CDS             2298..2933
                     /locus_tag="PLY05_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02883.1"
                     /translation="MKKIICRCLMVMMFSMQIIACTNSDVALVKSGVMSAYNKNTVGN
                     VFEASFDEAQWQAVTTKKGERVVRFTGKISQDLHDAVVADVEQKTQTLKVYQQEELKI
                     QYIQQLVKKQGIDGILFQQLNAEYGCVLRESSLAGGVIWWTPTCQDNANWGKYLEAAK
                     QAFLAQAWQVGDTVEVEWAISADGKQFDVTQMASPSWEGLAYEHILAAIYR"
     gene            2902..3456
                     /locus_tag="PLY05_05955"
     CDS             2902..3456
                     /locus_tag="PLY05_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNC02884.1"
                     /translation="MNISWRQFIVNLIKFMGSMMNIKEVLDELFSKPSALGNTSYQIK
                     PPMLLSDGLAQIYQAWELTGIYLLLEYQEGSDWNVALEQASGELLYVGKTQKNLCERI
                     KSHFGMKNLTIKTCENHRWHSVPSISDELKNRLSQGKVVIYPVECISDNLSAKALLPE
                     LIEKQLLVAFVLTHGHLPKLNLQF"
     gene            3540..4223
                     /locus_tag="PLY05_05960"
     CDS             3540..4223
                     /locus_tag="PLY05_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HNC02885.1"
                     /translation="MSELLTKYFFELNARLAVTQTIRREVDKRFSPDFNLFNLLLKDE
                     LGLSWFMRELLESNSAHGQQKLFRALFLQMIQEKQPHQANNKTLEILASQDDVQVNTE
                     VQTIYSKSSQRRMDILIHNRDYGLMIENKPWAADQHQQLTDYAEDLKKRFAGNFYIVY
                     LSPQGTDPSPESISTKELNKLKDEQRFYAMSLRDDLAYWARRCAEKSESEKMRQILLD
                     FSSYLSSTF"
     gene            4240..4794
                     /locus_tag="PLY05_05965"
     CDS             4240..4794
                     /locus_tag="PLY05_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02886.1"
                     /translation="MTIQQDEIISHALSSASNLELTLMIGQSYDTLRSKIIQNFASYF
                     QEKLQSAGWETEFKWWLEAPLGNYTGFYCRRKNWPPQLQLLVESKGTTGKYIYAVLKD
                     TDTLTHELFVNNLNMIKGKGKQSKICVWYQDLSTRFYDFTQKETLCLLYEKAEFAEYL
                     LAEIESLGSLIDEALLAKGIIHDR"
     gene            4784..>5230
                     /locus_tag="PLY05_05970"
     CDS             4784..>5230
                     /locus_tag="PLY05_05970"
                     /inference="COORDINATES: protein motif:HMM:NF020952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNC02887.1"
                     /translation="MTDDELTIVKGMIYGSKRIPIGYLQRKYRIGYNAAYRLITPLVE
                     EGLISPILANQPCVFNWEHQGWQEIAKQRHLWQPWALSAAEPKFFCDEEIEEDKCWAM
                     EQYDGRFAEYLAVFDTFTEALEWMLYEYVEMRDLLLQKTREFLQGKG"
BASE COUNT         1741 a          785 c         1139 g         1565 t
ORIGIN      
        1 cataaaccag acttggtcac gtctaaactg actgtaagca tcaagtaggg aagtatcatg
       61 agtggtaaaa atcaattgtg cgccatgatg attaatatca ggtctatgaa ataatcgaac
      121 cagagcttgc actaataggg tatgcaggtt ggtatctaat tcatcgatga ccagagttaa
      181 gcccttggct aaaatatcta aaacagggcc agctaagaat aataaattgc gtgttccatt
      241 tgacttatct cccaaatcaa aagtagtttg tattagcgat ttttcttatt ttaagatcag
      301 aaatttattt caccttcgac tcatatccga catattttgt cggatagtcc tgtatgctgt
      361 ctaaagatgt ataaccgagt tgtgaatcat gccaaaagca aataaagaaa atgctatcta
      421 caggcagtgg gctatcttaa aaattttatc ttcacaacgc cgtttagaaa cacaacaaat
      481 tcatcagcta ttactagagc agcatgagat tgctgtccat gaaaatactg tgcttagaga
      541 tttaaaaaat ttagagaaaa ttgggtttcc tctaaatcgt agtgacacta aaccgattga
      601 ttggtggttg aaaaaggaat ggcaagaaaa aattgcaggg atgacagagg gcgaggcttt
      661 gttagccatg ttagtgaaag agtatttaaa ggaaatttta ccaataaata cgctaaagca
      721 attggatgat ttatttgagt tggcagagaa aaagcttaat tcacaacaaa ataatcacgc
      781 aagtcaatgg ttagctaaaa tcagagtgat tgccgcacaa cagcctcaac taccaccagt
      841 ggttagagac agtgtaaaag cggtgattag tgatgcctta atgcacaacc aagtgattaa
      901 agcccagtat aaaagtcaca tttttttgtt gtcaccattg gcattattga tgcgtggaca
      961 ggttatttat ttggcagccg tagaaggcga tgatgtgacg caggtgaagc attttgcatt
     1021 acatcgtttt gactcagcca gtattgctta tggtgaaaag tttaatagtc cagaaaattt
     1081 taacattgac gaattgctta aagcaggatg gggacatttt caacaagctg accaagaaaa
     1141 aaacattcgt ttggtatttt ggtgtgatcg ctatttaaaa gatcatttag cagaaatgcg
     1201 tttagcggca aatcagtgga tagattttac aaatccacaa gatgatcact atatggtcac
     1261 ggtgaagttg ccttacacat ggcaattaaa acaatggtta ttgtcacaag gcagtaaagt
     1321 acgggttgtt aagcctattt ggttgcgtga ggctttacaa gaagaaatac aggctatgct
     1381 gaataattat aagtcctaaa tcgtatctga cgtattttgt cggatcggtt gggataataa
     1441 aagccctgtt aaaaaattag ataataagat gtttcacttt gtatttgctt ttaaaaatat
     1501 atgatgagaa tctatggtta gttgatgttt tattaagggg aaaataatgt tggaattaga
     1561 tttagagaat ttagatgtct cttttgatga tgagttttta aaaaatgaaa aactgacttg
     1621 gctaccgtgg gttggcgtaa attttcaaac aaccacagtg cgaactctag tgttaggtga
     1681 aagcacctat aattggaatc catccagtca gactgtgcaa gaccgtatca aaaaaaataa
     1741 ccatttaagg ttagtacacc aaaataatgc cataaaattt aaagcaaaat cggaatatac
     1801 ccgaaacttt gaacgtgcgg tgtttaataa caaaaaagtt acgccactca tggccaagca
     1861 actttggcat tcggtggttt atcataattt ggtgttacgc tgcatgaaag atgtgaaagc
     1921 gagaccaaaa tatgacgatt atctagtagg ttggcctgaa acctttaagc tttttgagtt
     1981 gctcagtgta gaacaatgta tttgttatgg cctagaagat aaaaaaatta aggcgtttaa
     2041 agaagcagcg acgttgtgtg gttatgaatt taaggtgcaa aaattttcta aaattggcaa
     2101 atacactccc aaaattatta ccttaaacat caataataag ttaataaaaa ttgcttttat
     2161 tagacatcct agctcgtttt tttcttggaa aaattgggca gtttttatga agaatacgcc
     2221 aattttcgta taagacacat tttgtcggat tggttgtgat aattaaacca tctaatgatt
     2281 tataggtggt tattgtgatg aaaaaaataa tttgtcgctg tttaatggtg atgatgttta
     2341 gtatgcagat aattgcttgt accaacagtg atgtggcttt ggttaagagc ggtgtgatga
     2401 gtgcttataa caaaaatacg gttggtaacg tatttgaggc ttcttttgat gaggcgcagt
     2461 ggcaggcggt caccactaaa aagggtgaaa gggtagttag atttacaggc aaaatttctc
     2521 aagatttgca tgatgcagtg gttgcagatg ttgagcaaaa aacacaaact ctaaaagttt
     2581 atcaacaaga agagttgaaa attcagtaca tacaacaatt ggttaaaaaa caaggtattg
     2641 atgggatatt gtttcagcag ttaaatgcag agtatggctg tgtgcttcgt gaatctagct
     2701 tagcaggtgg tgtaatttgg tggacaccaa cgtgccaaga taatgccaat tgggggaagt
     2761 atcttgaagc cgctaaacag gcttttttag cacaggcttg gcaagttggc gatacggttg
     2821 aagttgaatg ggctattagt gcagacggta aacaatttga tgtgacgcag atggcaagtc
     2881 cttcttggga aggcttagcc tatgaacata tcttggcggc aatttatcgt taatctaatc
     2941 aagtttatgg gtagcatgat gaacattaaa gaggtattag atgagttatt cagtaaacca
     3001 tctgctttgg ggaatacgag ttatcaaatt aagcccccaa tgcttttatc tgatgggcta
     3061 gcacagattt accaagcatg ggaattaact ggtatttatt tgttgctgga gtatcaggaa
     3121 ggtagtgatt ggaatgtagc tttagagcaa gcaagtggtg agctgctgta tgtaggtaaa
     3181 acgcaaaaaa atctgtgtga acgaattaaa agccattttg gaatgaaaaa cttaacaatt
     3241 aaaacatgtg aaaaccatcg ttggcactct gtgccatcaa tttctgatga gcttaaaaat
     3301 aggctctcac aaggaaaagt agtgatttat cctgttgagt gtataagtga taatttaagt
     3361 gcaaaggcat tgttgcctga gttaatcgaa aagcagttat tagtggcttt tgtattaacg
     3421 catggccatt tgccaaagtt gaatttgcaa ttttagaaac caatgtcaat cgcaaaaaac
     3481 atcttggttg gtgggtaaaa caatattcgg cctaatatgt agctgaaaag gagtttgaaa
     3541 tgtccgaact attaacaaaa tatttttttg aactcaatgc gcgcttagcc gtgactcaaa
     3601 ccattcggcg cgaagtagat aaacgatttt ccccagattt taatctattt aacttattac
     3661 taaaagatga gctaggtttg tcgtggttta tgagagagct tttagaaagt aactcagcac
     3721 atggccaaca aaagttattt agagccttgt ttttacaaat gatacaagag aaacaaccac
     3781 atcaggctaa caacaaaaca ttagaaatat tagcgagtca agatgatgtg caagtgaata
     3841 cggaggttca aaccatttac agtaaatctt cgcagcggcg tatggatata ctaattcata
     3901 atcgtgatta tggtttgatg attgaaaaca aaccgtgggc agcagaccaa catcagcaac
     3961 ttacagatta tgccgaagac ttaaaaaagc gatttgcagg taatttttat attgtgtatt
     4021 tgagtccaca aggtactgac ccatcgccag aaagtatttc gacaaaagaa ctaaataaac
     4081 taaaagatga gcaacgattt tatgccatgt cattacgaga tgatttggcg tattgggcaa
     4141 gaagatgtgc tgaaaagtct gagtctgaaa aaatgcggca gattctgtta gatttttcta
     4201 gctatttatc atcaacattt taatttataa ggaattgtga tgaccattca acaagatgaa
     4261 attattagcc atgccttaag cagtgccagt aatttagagt taacgctgat gatagggcaa
     4321 tcgtatgaca cattacgcag caaaattatt caaaactttg ccagttattt tcaagagaaa
     4381 ttacaaagcg ctggttggga gactgagttt aagtggtggc ttgaagctcc tttagggaat
     4441 tatacaggat tttattgtcg tagaaaaaat tggccgccgc aacttcagct tcttgttgag
     4501 tcaaaaggaa cgacaggtaa atatatttat gctgttttga aagatacaga taccttgacc
     4561 catgaattgt ttgttaataa tttgaatatg attaagggta aaggaaaaca aagcaaaata
     4621 tgtgtatggt atcaagattt gtcgactagg ttttatgact tcactcaaaa agaaacgctc
     4681 tgtttgttat atgaaaaagc cgagtttgct gagtatttgc ttgccgagat tgaatctttg
     4741 ggaagtctaa tagatgaggc gttattagcc aaaggtatta tccatgacag atgatgaact
     4801 caccattgtc aaaggtatga tttatggctc taaacgcatt cctataggtt atcttcaacg
     4861 caaatatcgc attggttaca acgcagcata tcggttaatc acaccattag ttgaggaagg
     4921 gttgataagc cccatcttag caaatcaacc ctgtgttttt aattgggagc atcaaggctg
     4981 gcaggagata gccaagcaac gtcatttatg gcaaccatgg gcgttgagtg cggcagaacc
     5041 taagtttttt tgtgatgagg agatagaaga ggataagtgt tgggcgatgg aacaatatga
     5101 tggccgattt gctgaatatc ttgcggtatt tgataccttt acagaagctc ttgaatggat
     5161 gttgtatgag tatgttgaaa tgagagactt gttattacaa aaaacgcgag agtttttgca
     5221 aggcaaaggt
//
LOCUS       DAOQMB010000135         5203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7270_length_5203_cov_15.621601, whole genome shotgun sequence.
ACCESSION   DAOQMB010000135 DAOQMB010000000
VERSION     DAOQMB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5203
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_5203_cov_15.621601"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(596..1873)
                     /locus_tag="PLY05_05975"
     CDS             complement(596..1873)
                     /locus_tag="PLY05_05975"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017385387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC02888.1"
                     /translation="MKLLSLNSCHVPDDLSTVTQYDPQSEMNPESVGMSDKQINKIWQ
                     ATEWLYKTGASPAITLCIRHKGKIILNRAIGHARGNGPEDTPDTPKILATPDTRICLF
                     SASKVVTAMLIHLLDELGEIDLLDPISYYIPEYGVNGKKNATIYHLLAHRGGIPSLPK
                     NTDPKLLFSPDSALDLLYKAKPIAPSGHRAAYHALTAGYILGEIIRRVTGKDSREFLA
                     EKISIPMDMPSFNFGLAPEYRDKVALNYSTGIKPVLLMDTFLRNILGGSMEDAEYYTN
                     DPQFMDTICPAGNIFATAEQASRFFQMLLDGGRYQDKQLMSPMTIRRATIEVARPEFD
                     AKLLLPMRYAMGPMLGAKPVGLYGLNTHHAFGHLGYTSIFCWADPDRDISASLLTTGK
                     FLVGTGLPALLHLIATISLQCKILPPSERRRFS"
     gene            complement(2058..2510)
                     /locus_tag="PLY05_05980"
     CDS             complement(2058..2510)
                     /locus_tag="PLY05_05980"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HNC02889.1"
                     /translation="MAKQSLITFFERIGLHLRTLLFDLNFQHYISLQIIPFCYLLVLI
                     STAGGLTFLVVTKFMQNVWLGLFYLCLSPMIFLIWVSICRVALELLIVVFRITAQLDH
                     VSSMNESLGEVTALTRPLSRLFKPNRPNSPPPSRPSREHTPPPTNHRR"
     gene            2787..3500
                     /locus_tag="PLY05_05985"
     CDS             2787..3500
                     /locus_tag="PLY05_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02890.1"
                     /translation="MKKFSTMLLITALLTPPLALAASNDEDLAEKQAESITIGSGWTL
                     IRNDTLRNIKTYAKQEDNKKFRSFKIDMIVDAPLETVAKVHFDVDNIKKWYWSTSESK
                     LLKKVSDTEYYYYQVYRTPIVPDRDVIIHAKIEPYTAEKGFMALNLEAVPDYMPIQGS
                     YVRMSAHNMAIKMTPIDNDKKTHLEVDGYIDPSGVSPAWAINFYQRKSPYITMVGLSR
                     MVQQPQYNGEAPTQFRYKR"
     gene            complement(3526..4050)
                     /locus_tag="PLY05_05990"
     CDS             complement(3526..4050)
                     /locus_tag="PLY05_05990"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNC02891.1"
                     /translation="MNLIKYAFLSLGLTLSQISLAANWVGTPAPAFNLSDQTGKVRQL
                     ADFKGKWLVMYFYPKNHTSGCTEEAKRFRDQYPQFQKQNINIVGVSLDSVESHKSFVK
                     DYNLPFTLLADTNKELAKKMGVLRGYGPVSFTSRETFLIDPQGTIVYHYDDVNPSAHA
                     TQVLADVAKLSAPK"
     gene            complement(4173..>5203)
                     /locus_tag="PLY05_05995"
     CDS             complement(4173..>5203)
                     /locus_tag="PLY05_05995"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNC02892.1"
                     /translation="LDAKQALIINGQPRFGYFDDALSTINSQDFAYETPFGSQRADWQ
                     KWLGFKEFQYFGGMSNQLIFGCAIAHLRYVAVAFVYVYDIEKRDLWSKSWRSPLGLGF
                     SLASNSLNGETHFKIPALVDIKLNYQDHPRQKTLIIKCKELHIEARMDESQLQPMSIC
                     TQTGYNGWTYTAKSAGQPLYGFVELKKQRFDLEKIKAYGSLDFSTGYMRRETWWNWAC
                     SAGEIQHGQRKGQSVGLNLSTGVNETSFSENCFWLNGKRYNLNHAQFEFDRKDTLKPW
                     KLTANNGQVQLNFQPMGMHREKVRAVYLNTHFRQMFGEFSGCIIVDGEEICLDGLKGF
                     VEDQYITW"
BASE COUNT         1659 a          957 c         1052 g         1535 t
ORIGIN      
        1 ttcatataca aataaacaac catcagtacc catgcactgg tactaaaatc atggttcatt
       61 gcataccaag gaatggcaat cagagcaata atcggggtgg acaataaaac actagcagct
      121 aaccagttaa cgggcggttt ttggttttga ctcatcgaga cactccaagc gcataaagcg
      181 agcaagagcc tagcagggga catgctaaac tctcaaattt gtgatagcta aaaccagaat
      241 agttttaaag cagctatctc ttgtaaattc agcacatagc tttggcaagg gctgtcgcta
      301 tatgcctagt agtttggtct aattggctgt gttctattta ataataggcg tatgacaata
      361 cgattattgc ttaaaaatac taaaagctga ttagggacaa taaatactaa aagtttaata
      421 tctattagcc aatcttgcca cgaaaatagt taatattttt ctagtgttag atccttaaag
      481 atcggcacta aaaacctatt gatagttttg cttgcaattt aatcaatttc tagctgagtg
      541 aatcttagcc aaataaaata caagtgtatt ttatttggcc attgtagcag cttttttaag
      601 aaaagcgacg tctttctgag ggagggagaa tcttacattg tagactaatg gtggcgatta
      661 aatgcaatag tgcagggagg cctgtgccta ccaaaaactt acctgtggtt aacaaactgg
      721 cagaaatatc acggtctgga tcagcccaac aaaaaatact ggtgtaaccc aaatgaccaa
      781 aggcatgatg agtgttaagg ccatataaac caactggttt tgcgcctaac attggcccca
      841 tcgcataacg catcggtaat aataatttgg catcaaattc gggacgggca acttcgatcg
      901 tcgcacgacg aattgtcatt gggctcatta attgtttgtc ttgataacgg ccaccatcta
      961 gtaacatttg aaagaagcgg ctggcttgtt cggctgttgc aaagatatta cctgctggac
     1021 aaatggtgtc cataaattga gggtcattgg tgtagtattc tgcatcttcc attgaaccac
     1081 ccaaaatgtt acgcaaaaag gtatccatta ataaaacagg tttaatacct gtactgtaat
     1141 tgagcgcaac tttatcacgg tattctggcg ctaaaccaaa gttaaaactg ggcatatcca
     1201 ttggaatact aattttttct gccaaaaact cacggctgtc tttgcctgtg acgcggcgaa
     1261 taatttcacc caaaatatag ccagcggtta aggcatgata ggcagcacga tggccactag
     1321 gggcaatcgg tttcgcttta tagagtaaat ctaaggcaga atcaggacta aataataatt
     1381 taggatcggt gtttttcggt aggctcggaa tgccaccacg atgggctaac aagtgataaa
     1441 tcgtggcatt ttttttgcca ttaacaccat attcaggaat gtaataacta atggggtcta
     1501 ataaatcaat ttcgcctagc tcatctaata aatgaatgag catagccgtg accacttttg
     1561 aggccgaaaa taaacaaata cgagtatcag gtgttgctaa gatttttggt gtatcaggtg
     1621 tatcttctgg gccattgcca cgagcatgac caatggcacg atttagaatg attttgcctt
     1681 tatgacgaat acagagcgta atagcaggcg atgcgccagt tttatataac cattcagttg
     1741 cttgccaaat tttattaatt tgtttgtcac tcatgcccac cgattcagga ttcatttcac
     1801 tttgtgggtc atattgagtc acagtgctta ggtcatcagg aacatgacaa ctatttaacg
     1861 ataataattt cataactatt ctcaggtcat tttaagaaca aaacagagtg gtgtctctat
     1921 tgcggttatg gacaaccgcg taagtcctac agatattgtg taattactat atcagcacca
     1981 tcgtactaaa aatagagaga attttaccga ttacgccaaa atgacactgc cctaaacgtg
     2041 gctttagggc agtgtgttta acggcgatga ttggtgggtg gtggcgtgtg ttcgcgtgat
     2101 ggacgtgatg gtggtgggct attagggcga ttaggtttaa atagtcgaga taggggacga
     2161 gttaatgcag tgacttcacc taaagattca ttcatgctag aaacatgatc gagttgggca
     2221 gtaattctaa aaaccacaat taataattcg agtgcgactc gacaaataga aacccaaatt
     2281 aaaaagatca ttggtgataa acacaaataa aataagccaa gccaaacatt ttgcataaat
     2341 ttggtgacca ctaaaaaggt aaggccacct gcggtgctga ttaataccaa tagataacaa
     2401 aaaggaataa tttgcaggct aatgtaatgt tgaaagttta ggtcaaacaa taaggtgcgt
     2461 aggtgtaagc caatacgctc aaaaaaagtg atgagtgatt gtttggccat aaacctataa
     2521 ctccagacaa aatttttaac aagttctaga gtgtacggct atcctgtatt gatgagaaga
     2581 gctattagtg cgttaactgt caaaaaaagc cgatgaatgg cattaattat gagataaaaa
     2641 cttttcgcta gttaattaag aaagtcatat aataaaattg tttcgatgaa ataataaagc
     2701 ttttttatta aagcaattaa taagatgcat caatttaagg agtgaggttg atgtatttag
     2761 ataaccacca aaagatgagt cctataatga aaaaattttc tactatgctg ttaattacag
     2821 cattattgac accaccacta gctcttgcag ccagtaatga tgaggatctt gccgaaaagc
     2881 aggctgaatc tattaccatt ggttcaggat ggacgctaat tcgtaacgat acgctgcgta
     2941 atattaaaac ctatgctaaa caagaagata ataaaaaatt tcgctcattt aaaattgata
     3001 tgattgttga tgccccttta gaaactgtgg ccaaagttca ctttgatgtg gataatatta
     3061 aaaaatggta ttggtcaacc agtgaatcaa aattactgaa aaaagtctca gacaccgaat
     3121 attattatta tcaagtctat cgcacaccga ttgtgcctga tcgtgatgtg attattcacg
     3181 ctaaaattga gccttatacc gctgaaaaag gctttatggc attaaatctt gaagccgtac
     3241 cagactatat gccgattcaa ggcagctatg tgcggatgag tgcgcataat atggcaatca
     3301 aaatgacgcc gattgataat gataaaaaaa cccatttaga ggttgatggt tatattgatc
     3361 caagcggtgt gtcaccagca tgggcgatta atttttatca gcgtaaatcg ccgtatatta
     3421 ccatggtggg cttatctcgg atggtgcaac agcctcaata taacggtgaa gcaccaacac
     3481 aatttcgcta taagcgttaa ttaaaaaata gagtgcctcg gcactctatt ttggtgcact
     3541 taatttagca acatcagcta gcacttgagt agcatgagca ctcggattga catcatcata
     3601 gtgataaaca attgtgcctt gagggtcaat taaaaaggtt tcacggctag taaaactcac
     3661 aggcccataa ccacgtaaca cgcccatttt tttggcaagt tctttgttag tatccgctag
     3721 taaggtaaag gggaggttat agtctttaac aaagcttttg tgggattcca cactgtctaa
     3781 actaactcca acaatattaa tgttttgctt ttgaaattgc ggatattgat ctcgaaaacg
     3841 ttttgcttct tcggtacaac cactggtatg atttttggga taaaaataca tgactaacca
     3901 tttaccttta aaatctgcga gttgtcgtac tttacctgtt tgatcactta agttaaaagc
     3961 gggtgcgggt gtgcctaccc agttagccgc taggctgatt tggctgagag ttaaaccaag
     4021 gctgagaaat gcatatttga ttaaattcac tttaatcttc ctcctttact gataacttat
     4081 ttttttcagg tgaaagttta tgaatgtggg ctgagcggag tcgaaaccag tgataacacg
     4141 tcgacttcac tcagtgtaga tggtaattgt cactaccacg taatatattg gtcttcaaca
     4201 aaacctttta agccatctaa gcagatttct tcaccatcca caataataca accgctaaat
     4261 tcaccaaaca tttgtcgaaa gtgggtattt aaatacacgg cacggacttt ttcgcggtgc
     4321 atacccattg gttgaaaatt cagttgtact tgaccattat tggcagtgag tttccacggt
     4381 tttaaggtgt ctttgcgatc aaattcaaat tgagcatgat ttaaattata acgtttgcca
     4441 ttgagccaaa agcaattttc actaaaactg gtttcgttga cacctgttga taaatttaga
     4501 ccaacagatt gacctttgcg ttgaccgtgt tgaatttcgc ctgcggaaca cgcccagttc
     4561 caccatgttt cacggcgcat ataacctgta gaaaaatcta agctgccata agctttgatt
     4621 ttttctaaat caaagcgttg ttttttcagt tcaacaaatc catataatgg ttggcctgcc
     4681 gatttagccg tatatgtcca accgttgtaa ccagtttggg tgcaaatgga catgggttgt
     4741 aactgacttt catccatacg tgcttcaata tgtagctctt tacatttgat aattaaggtt
     4801 ttttgtcgag gatgatcttg ataatttagt ttaatatcga ctaaggcagg aattttaaaa
     4861 tgggtttcac cattgaggga gttactagcc aagctaaagc ctaaacctaa tgggctacgc
     4921 cacgatttag accataagtc gcgtttttca atgtcataca cataaacaaa agcaacagcg
     4981 acataacgta aatgggcaat cgcacagcca aaaattaatt gattactcat gccaccaaaa
     5041 tattgaaact ctttaaagcc tagccatttt tgccaatcag cacgttgact gccaaaaggg
     5101 gtttcgtagg caaagtcttg gctattaatg gtgcttaaag catcatcaaa ataaccaaag
     5161 cgaggttgac cattgatgat taatgcttgt ttggcatcga gag
//
LOCUS       DAOQMB010000136         5196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7279_length_5196_cov_14.695974, whole genome shotgun sequence.
ACCESSION   DAOQMB010000136 DAOQMB010000000
VERSION     DAOQMB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7279_length_5196_cov_14.695974"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="PLY05_06000"
     CDS             complement(<1..568)
                     /locus_tag="PLY05_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004707608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNC02893.1"
                     /translation="MSRLILFNKPYGVLCQFRRTDERSTLADFFSDPAIHPCGRLDFD
                     SEGLLLLTDDGQLNARITEPKYKLPKTYWAQVDGEITDEAIKQLKQGIMLNDGMTMPA
                     IVKKIAEPAIWQRDPPIRYRANIPTSWIELTITEGRNRQVRRMTAAVGFPTLRLIRAQ
                     IGEWQINDLALGMSKEINVSLGRIKQVNH"
     gene            785..2047
                     /gene="icd"
                     /locus_tag="PLY05_06005"
     CDS             785..2047
                     /gene="icd"
                     /locus_tag="PLY05_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HNC02894.1"
                     /translation="MGYQKIVVPADGDKITVNADLSLNVPNFPIIPYIEGDGIGVDIS
                     PVMIKVVNAAVSKAYGSKRAISWMEIYCGDKSARLYGNNNYLPEETLEALREFVVSIK
                     GPLSTPVGGGLRSLNVALRQELDLYVCQRPVRWFKGVPSPVSHPELTDMVIFRENSED
                     IYAGIEWKADSPEAIKVIKFLREEMGVKKIRFPEGCGIGIKPVSKEGTQRLVRKAIQY
                     AIDNDKDSVTLVHKGNIMKYTEGAFRDWGYELAAERFGAELIDGGPWMSLRNPKTGKN
                     IIIKDVIADAFLQQILMRPAEYSVIATLNLNGDYISDALAAEVGGIGIAPGANIGGSI
                     AMFEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIITGTQGAISAKTVT
                     YDFERLMPDATLLRCSEFGDAIISHMED"
     gene            2327..3283
                     /locus_tag="PLY05_06010"
                     /pseudo
     CDS             2327..3283
                     /locus_tag="PLY05_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005239827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            3321..3797
                     /gene="ybeY"
                     /locus_tag="PLY05_06015"
     CDS             3321..3797
                     /gene="ybeY"
                     /locus_tag="PLY05_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNC02895.1"
                     /translation="MTKSLLTLSLQKATEQKSLPTNKDFKEWILQALAVANYAKPCEV
                     AIRLVDEEESHALNLQYRGKDKPTNVLSFPSDLPNEVLTSLKREPLGDIIICVPVVLA
                     EATEQHKTATAHWAHLTIHGTLHLLGFDHIDDEEAEEMEALEIDALQELGINNPYE"
     gene            complement(3902..>5196)
                     /locus_tag="PLY05_06020"
     CDS             complement(3902..>5196)
                     /locus_tag="PLY05_06020"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004719531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNC02896.1"
                     /translation="GVEKMTTTVRRVAIIGGNRIPFARSNGAYANVSNQDMLTAALNG
                     LVERFNLQGVRVGEVVAGAVLKHSRDFNLTRECVLSTKLSPATSAYDIQQACGTGLEA
                     AILVANKIALGQIECGIAGGVDTTSDAPLALNDDLRKILLEINRAKTNQDRLKAALKI
                     RPKHLAPEQPKNGEPRTGLAMGDHAAITALEWGITREDQDQLAFNSHKNMAAAYDRGF
                     FDDLVTPFQGLKRDNNLRKDISLEKLATLKTCFGKGEGATMTPANSTPLTDGASVVLL
                     ASEEWAKERGLPVLAYLSFTEVAAVDFVHQKEGLLMAPAYAVPRMLERAGLKLQDFDY
                     YEIHEAFASQVLSTLKAWEDTKFCKERLGLKKPLGKIDRSKLNVNGSSLAAGHPFAAT
                     GGRIIATLAKMLNEKGSGRGLVSICAAGGQGVVAIIEK"
BASE COUNT         1600 a         1054 c          998 g         1544 t
ORIGIN      
        1 agtgatttac ctgcttaatt cgccctaaac tcacattaat ttcttttgac atacctaatg
       61 ctaagtcatt aatttgccat tcaccaattt gtgctctaat caaacgtaag gttggaaaac
      121 cgactgccgc tgtcatgcgc cttacttgtc tattacgccc ttctgtaatt gttaattcaa
      181 tccaactagt tggaatattg gcacgataac gaatcggagg atcacgttgc caaattgctg
      241 gctcggcaat ttttttaaca atagcaggca tagtcatacc atcatttaac atgatacctt
      301 gttttaattg tttaatcgcc tcgtcagtaa tttctccatc cacctgcgcc caataggttt
      361 ttggcaattt atatttgggc tccgtaatac gcgcattgag ttgaccatca tccgttaata
      421 gcagtaagcc ttctgaatca aaatctaaac gaccgcaagg atgaatcgct ggatcagaga
      481 aaaaatctgc taatgttgag cgctcatcag tacgtctaaa ttgacataaa acgccataag
      541 gtttattaaa taagattaga cgactcatgt aaactctcaa aaatccaaca acaaatatca
      601 tttaaaatta tagtgatatt tattataaaa atgaattttg gcaaaaaaaa tctttgaaat
      661 acgattaaca taatcataat ttaaatctaa ggctaaacct taaatttata agcaaactgg
      721 cattaccatg ctaccatttt taatagactt ttgattttat taataacaag ggagtacctg
      781 cacaatgggt taccaaaaga ttgtagttcc agccgatggc gacaaaatca ccgtaaacgc
      841 tgacctttcc ttaaatgtac caaactttcc aattattcct tacatcgaag gtgatggtat
      901 tggggttgat attagccctg tcatgattaa agtggtcaat gccgccgtta gcaaagctta
      961 tggtagcaaa cgtgcgattt catggatgga aatctattgt ggtgataaat cagcaagatt
     1021 atatggcaat aataattatt tacctgaaga aactttagaa gccttacgcg agtttgtggt
     1081 gagtattaaa ggccctttat ccacaccagt tggcggtggt ttacgctcat taaacgttgc
     1141 tttacgtcaa gagttagatt tatatgtttg ccaacgtcca gtacgctggt ttaaaggcgt
     1201 acctagccca gttagtcatc cagaattaac tgacatggtt atcttccgtg aaaactctga
     1261 agatatttat gcgggtattg aatggaaagc tgatagccca gaagcgatta aagtcattaa
     1321 gtttttacgc gaagaaatgg gcgtaaagaa aattcgtttc cctgaaggtt gtggtattgg
     1381 tattaagcca gtttctaaag aaggtactca acgtttagtg cgtaaagcca ttcaatatgc
     1441 gattgataac gataaagact ctgtgacttt agttcataaa ggcaacatca tgaaatacac
     1501 cgaaggtgca ttccgtgatt ggggttatga gttagctgct gagcgttttg gtgctgagtt
     1561 aattgatggt ggcccttgga tgagtctaag aaatccaaaa acaggcaaaa acatcatcat
     1621 taaagacgtg attgctgatg cgttcttaca acaaatctta atgcgtcctg ctgaatactc
     1681 tgtcattgca actcttaact taaacggcga ctatatttct gatgcgttag ccgctgaagt
     1741 gggtggtatt ggtattgcac ctggtgcaaa cattggtggt tctattgcca tgtttgaagc
     1801 cacacacggt acagcaccaa aatatgctgg ccaagataaa gtgaatcctg gctcgattat
     1861 tttatctgca gaaatgatgt tacgtcatat gggctggact gaagcggctg atttgattat
     1921 tacaggtaca caaggcgcaa ttagtgccaa gacagttact tatgattttg aacgtttaat
     1981 gccagatgca accttattac gttgttctga atttggcgat gcgatcatta gtcatatgga
     2041 agactaagtc gttaaaatag acaatatcga taaaaagaga caggataact gtctcttttt
     2101 tctttttcgc cctttccata attttgtcca aaatgtcata ataagcagct attattaaaa
     2161 ataacaactc tggacttacg gatttcacct tcaggcacgc gtttggtgaa cataaaaccg
     2221 ttgaaaacgg ttgaaatgtg aacaaataaa cgataatcgc ataagtccta atttgttaat
     2281 aatattttta ggagcgagtt ttcgtttgaa cagcagcgtc acaactattc gtcacttaca
     2341 cttagagcct caacagcctc aacgcttgca cgccctatgc ggtgcatttc atcaaaacat
     2401 taaacaaatt gaaaaacgtt tgggaattca tattcatcat cgtggttatc attttcaatt
     2461 acacggttct ttaaacgatg tcactcatgg cgagcaactg ttaaaacgct tgtatgaaga
     2521 aaccgcgcaa agcccagaaa ttagctcaga aatggtgcat cttatgttac aaactagcca
     2581 agctccactc acaaatgata cagaagacga tcattctgac gataaatttc ctgtgttata
     2641 tagccgcaaa ggtggtattc gcccacgcgg acataatcaa gccaactacg tcaaaaatat
     2701 tttaaagcat gatattagtt ttggggttgg ccctgcaggg actggtaaaa cttatttagc
     2761 cgttgcggca gccgttgacc aactcgaaaa agatattatt aaacgaattt tattggtacg
     2821 ccctgccgtc gaagcaggtg aaaaactcgg ctttttacct ggtgatttat cagacaaaat
     2881 tgatccctat ttacgtcctt tatatgacgc cttatatgaa atgcttggtt ttgaacgtgt
     2941 tgccaaactg attgaaaaac aagtgatcga agttgcgccc ttagcctata tgcgcggtcg
     3001 caccttaaac aatgcttttg tcatcttaga cgaagcacaa aatactacgc ctgaacaaat
     3061 gaaaatgttt ttgacgcgtt tgggttttgg ttctcgagcc gtgattactg gtgatattac
     3121 ccaagttgat ttaccacgtg gtcatgagtc aggcttagca cactgcttac gcattttaac
     3181 cgatgttgac ggcattcata ttacgcgttt tagctctaaa gatgtagtac gtcatcattt
     3241 agtgcaacgc attgttgaag cctatgaagc atgggacaaa gaacatcctg tcacaccgcc
     3301 accaccacga agccaacgtt atgactaaat cactcctcac tttatcctta caaaaagcca
     3361 cagagcagaa atctttaccc acaaataaag actttaaaga gtggatttta caagcattgg
     3421 ccgttgcaaa ttatgctaaa ccttgtgagg tagccattcg tttagtggat gaagaagaaa
     3481 gtcatgcact caatttacaa tatcgtggca aagataagcc gacaaatgta ttatcttttc
     3541 cgagtgattt gcctaatgaa gtcctgacct cactaaaacg agaaccttta ggcgacatta
     3601 ttatttgtgt acctgttgta cttgctgaag cgactgaaca acacaaaacc gccaccgctc
     3661 attgggcgca tttaaccata catggtactt tacatttatt aggttttgat cacattgatg
     3721 acgaggaagc cgaagagatg gaagctttag aaattgatgc tttacaagag ttaggtatta
     3781 ataatcctta tgaataaata ggttctgttg atgttttgcg tgtgaaccac aatcaaaaca
     3841 ccaacagaac ctaatgttac ctctctttta aaagagaggt aactgactgg aagatgccta
     3901 attatttttc aataatcgca accacacctt gaccacccgc agcacaaatg gacactaagc
     3961 cacggccact gcctttttca tttaacattt ttgctaaagt agcaatgata cggccacctg
     4021 ttgctgcaaa tggatgacct gcggctaaag aagaaccatt gacatttaat ttgctacgat
     4081 caattttacc taaaggcttt ttcaaaccta aacgctcttt acagaacttg gtgtcttccc
     4141 atgcttttaa ggtagacaat acttgagaag caaaagcttc gtggatttcg taataatcaa
     4201 aatcttgtaa cttcaagcct gcacgctcta acatacgagg cacagcataa gctggagcca
     4261 ttaacaagcc ttctttttga tgcacaaaat caaccgcagc cacttcggta aagcttaaat
     4321 aagctaatac tggtaaacca cgctcttttg cccactcttc agaagccaat aacaccactg
     4381 aagcaccatc tgttaatggt gtggagtttg ctggcgtcat ggttgcgcct tcacctttac
     4441 caaaacaggt ttttaaggtc gctaattttt ctaaggaaat atccttacgt aaattgttgt
     4501 cgcgctttaa accttggaat ggagtcacta aatcatcaaa aaaaccacga tcataagcag
     4561 cagccatgtt tttatggctg ttaaaggcta attgatcttg gtcttcacga gtaatacccc
     4621 actctaaagc ggtaatcgca gcatgatcac ccattgctaa accagtacgt ggctcaccat
     4681 tttttggttg ttctggtgct aagtgttttg gacgaatttt taaggcggct tttaaacgat
     4741 cttgattggt tttagcacgg ttgatttcga gtaaaatttt acgtaaatcg tcatttaaag
     4801 ccaaaggtgc atcactggtt gtatctacac cacctgcaat accacattcg atttggccta
     4861 aagcaatttt gttggccact aaaatggcgg cttctaaacc tgtaccacac gcttgttgaa
     4921 tatcataagc agaggttgct ggcgataatt tggtacttaa tacacattca cgggttaagt
     4981 taaaatcacg gctgtgcttt aatactgcac cagcaacaac ttcaccaaca cgtacgcctt
     5041 gtaaattaaa acgctctacc aagccattta aggccgctgt taacatatct tggttggaaa
     5101 cattagcata agcgccgtta gaacgggcga aaggaatacg gttaccgccg ataatagcaa
     5161 cacgacgtac agtagtagtc attttttcta ctccaa
//
LOCUS       DAOQMB010000137         5195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7282_length_5195_cov_14.721790, whole genome shotgun sequence.
ACCESSION   DAOQMB010000137 DAOQMB010000000
VERSION     DAOQMB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7282_length_5195_cov_14.721790"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PLY05_06025"
     CDS             complement(<1..1327)
                     /locus_tag="PLY05_06025"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HNC02897.1"
                     /translation="MSTTKQTRCPHCTSVFNITDDQLAARGGHVRCGSCLQVFRADQH
                     LVDASQIVDIPAASPQAAAPISTPPVAPKNTSDSQMSMPVVAKEQHAPPQPQPAPAKK
                     RKKDDESWAMDLLGDLGAELEDDLANINPQKSVSAPISEPEEKPQPSKTKKPLFDDEI
                     SDLLQEAWVEPTDSAPLKGLGGVDKIKESADESWAQALMSELEEEEKKEKAKNYSMEL
                     APQRPKDPNAKTKTNEPTPEKTDTETSAKKSANSSNQDDDLLSFLNKSASTTAQPQRT
                     NATLPLEIKSQPMISINWGYWLTWSFMCIFATILLLVQYVYFHFNELAFDEKTRPQLV
                     QICSTLGCQVPEPPNASLISIEKLVIRKHPEIKEALQINAVVFNKADFAQPLPALKLT
                     LLNKKREVTAARAFQPKEYLHGDAAKILRRIPPETPIQIQLDIVNPKVDM"
     gene            complement(1495..>2016)
                     /gene="prmA"
                     /locus_tag="PLY05_06030"
     CDS             complement(1495..>2016)
                     /gene="prmA"
                     /locus_tag="PLY05_06030"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNC02898.1"
                     /translation="QPNAINLLLDPGLAFGTGTHPTTALCLEWLDGLDLQDKIVVDYG
                     CGSGILAIAALLLGAKHAYCVDTDPQALLATKDNAARNGVADKMTVCFPHDMPAIKAQ
                     TLVANILAGPLAELAPILADLCQTDANIALSGIIDSQVEDLREAYQPWFVLDQLQTKQ
                     ENWCRLSGKRLAN"
     assembly_gap    2017..2116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2206..3546)
                     /gene="accC"
                     /locus_tag="PLY05_06035"
     CDS             complement(2206..3546)
                     /gene="accC"
                     /locus_tag="PLY05_06035"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003652264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HNC02899.1"
                     /translation="MLSKVLIANRGEIALRVLRACKELGIRTVAVYSKADRDLLHVRL
                     ADESLCIGPALSKESYLNIPSIIAAAEATQAQAIHPGYGFLSENADFAEAVEQSGFTF
                     IGPRADNIRMMGNKVAAIEAMKAAGVPTVPGSDGAVNADSAVAIAQRIGLPIIIKAAA
                     GGGGRGMRVVHHEDEILNSLAITQSEAKAAFGDDTVYMEKFLQHPRHVEIQVLGDGEG
                     NAIHLGDRDCSLQRRHQKVVEEAPAPHIPDNVRAEVADACVKACQQMKYRGAGTFEFL
                     YEDGRFYFIEMNTRVQVEHPVTEMVTGVDIIKEQLRVAAGLGLSYRQEDIIIRGHAIE
                     CRINAEDPQSFLPCPGKINYYHAPGGLGIRMDSHIYTGYSVPPYYDSLIGKLIAFGNS
                     RAVALQRMRNALDEIVVEGIKSNIPLHRDIILTDAAFNEGGVDIHYLEKKLGLS"
     gene            complement(3554..4027)
                     /gene="accB"
                     /locus_tag="PLY05_06040"
     CDS             complement(3554..4027)
                     /gene="accB"
                     /locus_tag="PLY05_06040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HNC02900.1"
                     /translation="MDIRKVKKLIEMLEESTFDELEIKEGEESIRIARNRQSGVVSYA
                     APVAPMPVATAPTMTSTTPAAQAIGTAPPEDKPQTQGFIQRSPMVGTFYRAPSPTADN
                     FVEVGQTVKQGDTLCIIEAMKMMNQIQADKSGTIESILVDNGQVVEYDQALFTIV"
     gene            complement(4043..4492)
                     /gene="aroQ"
                     /locus_tag="PLY05_06045"
     CDS             complement(4043..4492)
                     /gene="aroQ"
                     /locus_tag="PLY05_06045"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004901488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNC02901.1"
                     /translation="MMAQILLLHGPNLNLLGTREPTIYGATTLAQINEQLATKAHQQG
                     YSLDYLQSNHEGVLVDKIQAAAHDGTQYIIINPAAFTHTSVAIRDAILAVKIPFVEVH
                     LSNVHARETFRHHSYFSDVAIGVICGLGAQGYDFALQYVINSLNHTK"
     gene            4772..5128
                     /locus_tag="PLY05_06050"
     CDS             4772..5128
                     /locus_tag="PLY05_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02902.1"
                     /translation="MILKPLLIFGVLSCLTIGQVYAEESSSVDAMMPNLVIDLSSVAS
                     QVWFAQAQIWGACSLVALLVAIFAWILLNINSLKQQDNQGDVLAKRVSLLWVAFLMVM
                     VVSFAIPLLRVWLVLA"
BASE COUNT         1461 a         1013 c         1055 g         1566 t
ORIGIN      
        1 tcatatccac ttttggattg acgatgtcca attgtatctg aataggtgtt tctggaggaa
       61 tacgtcggag aatttttgct gcatctccat gtaaatattc tttaggttga aaagcgcgcg
      121 cggcagtgac ttcacgtttt ttgtttaata aagtcaattt gagtgcgggt aatggttgtg
      181 caaaatctgc tttattaaac acaactgcgt tgatttgtaa tgcttctttg atttcgggat
      241 gtttacgaat aacgagtttt tcaatactta tcaaagacgc attcggcggc tctggtactt
      301 ggcaacctag agtgctacag atttgtacta actgtggacg tgttttttcg tcaaaagcta
      361 gttcattaaa atgaaaatag acatattgca ctaataataa aatagtggca aaaatgcaca
      421 taaagctcca cgttaaccaa taaccccaat tgatagaaat catgggttgt gacttgattt
      481 ctagagggag ggtagcgttg gtgcgttgag gttgagcggt tgtgctagca ctcttattta
      541 aaaagcttaa taagtcatcg tcttgattgc tactatttgc cgactttttc gcacttgttt
      601 cagtgtcagt tttttctggt gtaggctcgt tggttttagt tttagcatta gggtctttag
      661 gtcgttgtgg cgcaagctcc atgctgtagt ttttagcttt ttcctttttt tcttcttctt
      721 caagctcaga cattaacgct tgtgcccaac tttcatcagc actttcttta attttgtcaa
      781 caccacctaa accttttagc ggtgcagagt ctgttggttc aacccaagct tcttgtaata
      841 aatcactgat ttcatcatca aataacggct ttttggtttt gctcggttgg ggtttttctt
      901 ctggctcact gatgggcgca gatacagatt tttgagggtt aatattggcc aagtcatctt
      961 ctaattcggc acctaaatcc cctaacaaat ccatcgccca agattcatca tcttttttgc
     1021 gttttttagc gggtgctggc tgaggttgtg gcggagcatg ttgttcttta gcgacaacag
     1081 gcatagacat ttggctgtct gatgtatttt ttggagcgac tggtggtgta ctgattggtg
     1141 cagcagcttg aggtgatgca gcgggaatat caacgatttg tgatgcgtca accaaatgct
     1201 gatcggctct aaatacttgc aagcagctac cgcagcgtac atgaccacca cgagcggcta
     1261 gttgatcatc agtaatatta aaaacacttg tacaatgagg acagcgtgtt tgtttggttg
     1321 tgctcatagt ccctcaagct ccaaatcttg tgctaaaaat atgggagaag tcgatattat
     1381 atattttttc attatttttg tggattattt cacagaataa tcggatcttt agcctatata
     1441 tcgccgatta tgcgccgaga aacaggattt tgctgtctat cgacgcacaa atatttagtt
     1501 cgccaaacgt ttacccgata aacgacacca attttcttgt ttagtttgta gttggtctaa
     1561 aacaaaccac ggctgataag cttcacgtaa atcttcaact tgactatcaa taatgccaga
     1621 taaagcaata ttggcatccg tttggcataa atcggctaaa attggagcta actcggctaa
     1681 agggccagcc aaaatattag caacgagtgt ttgagcttta atggcaggca tatcatgtgg
     1741 gaaacacacg gtcattttgt cggcgacacc attacgcgca gcattatctt tggtggctaa
     1801 taaagcttga gggtcagtat cgacacaata agcatgtttt gcaccgagta ataaagccgc
     1861 aatggctaaa atgcctgagc cacatccata atcgacaaca atcttatctt gcaaatctaa
     1921 accatctaac cattctaaac ataaggcggt ggttgggtgt gtacctgtac caaaagccaa
     1981 accagggtct agtaataagt taatagcatt aggttgnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnngcgc cagcacctac cagttcgagg gcttcttcaa tacggtctgc
     2161 atcagctttt gagctgtcga ttttaagttg taaccaagcc atgctttatg ataatcccaa
     2221 ttttttctca agataatgaa tgtccacacc accttcgtta aaggcagcat cagtgaggat
     2281 tatatcacga tggagcggaa tattgctttt aatgccttca acaacaattt catcgagtgc
     2341 gtttctcatg cgttgtaaag caacagcacg actattacca aacgcaatta atttgccaat
     2401 caatgagtca tagtagggtg gcacgctata gcccgtataa atatgagaat ccatgcgaat
     2461 gcctaagcca cccggcgcat ggtaataatt gattttacca ggacagggca aaaagctttg
     2521 tgggtcttcg gcattaatac ggcattcaat cgcatgacca cgaatgataa tatcttcttg
     2581 acgatacgat aaacctaaac ctgcggctac ccgtaattgt tctttaataa tatcgacacc
     2641 agtcaccatt tcggtaacag ggtgttcaac ttgcacacgc gtattcattt cgatgaaata
     2701 aaaacggcca tcttcataca aaaattcaaa agtccctgca ccacgatatt tcatttgttg
     2761 acaagctttg acacaagcat cagcaacttc ggcacgaaca ttatcgggaa tatggggagc
     2821 aggggcttct tctacgactt tttgatggcg gcgttgtaaa gaacaatctc gatcacctaa
     2881 atgaatggcg ttgccttcac catcacctaa cacttgaatt tcgacatgac gtggatgttg
     2941 taaaaacttt tccatgtaaa cggtgtcatc accaaatgct gctttggctt cagattgagt
     3001 aatggcaaga ctatttaaaa tttcatcttc gtgatgaacc acgcgcatac cacgaccacc
     3061 accacccgca gctgctttaa taatgatggg taaaccaata cgttgagcaa tagcaacggc
     3121 actatcggca tttactgcac catctgaacc aggtacagtg gggactccag ccgctttcat
     3181 cgcttcaatg gcggcaactt tattacccat catccgaata ttgtcagcac gaggaccaat
     3241 aaaggtaaag ccagactgtt caaccgcttc ggcaaaatca gcattttcgg acaaaaaacc
     3301 ataaccaggg tgaatggctt gggcttgcgt ggcttcggca gcagcaataa ttgacggaat
     3361 gtttaagtaa ctttctttgg aaagtgcagg gccaatacat agcgattcat cggccaaacg
     3421 aacgtgtaat aaatcacgat cagctttaga gtacaccgca acggtgcgga tacctaattc
     3481 tttgcaggct cgtaacacgc gcagtgcaat ttcaccacgg ttagcaatta agactttgct
     3541 tagcatagcg gcctcataca atagtaaata aggcttggtc gtattcaacc acttgaccgt
     3601 tatccactaa aatggattca atcgtgcctg atttatcagc ttgaatttgg ttcatcattt
     3661 tcatcgcttc aataatacaa agcgtatcac cttgtttaac ggtttgaccg acttcgacaa
     3721 aattgtctgc agttggtgac ggagcacgat aaaatgtacc aaccatagga gagcgttgga
     3781 taaaaccttg cgtttgtggt ttatcttcgg gaggtgctgt accaatcgct tgagcagcag
     3841 gagttgttga tgtcattgta ggtgctgtag caacaggcat tggtgcaact ggcgcagcat
     3901 aactaacaac acccgattga cggttacgag caatacgaat cgactcttca ccttctttaa
     3961 tttctaattc gtcaaaagtg gattcttcta acatttcaat taatttttta actttacgaa
     4021 tatccataaa atactctcta aattattttg tgtgatttaa actgttaatg acatattgca
     4081 aggcaaaatc atagccttgc gcacctagtc cacaaatgac cccaatggca acatccgaaa
     4141 aataagaatg atgtctaaag gtttctcgtg catgaacgtt ggacaaatgg acttctacaa
     4201 aaggaatttt taccgctaaa atcgcatcac gaatggcaac agaggtatga gtaaaggcag
     4261 caggattaat aataatatat tgcgtaccat catgagcggc ggcttgaatt ttatctacca
     4321 atacaccttc atgattactt tgcaaataat ctaagctata gccttgttga tgcgctttgg
     4381 tagcaagttg ttcgttaatt tgcgctaacg tggttgcacc ataaatagtt ggctcacgtg
     4441 ttcctaatag atttaaatta ggcccgtgta acaacaaaat ttgtgccata attaatctcg
     4501 ttttgctgaa ttaaaattga ggcaaaggca ttttatcaaa ataactgctt aaaccttaaa
     4561 atgtgctata tgctagattc ttgttatttc atcaggtatc ttgagcctga tattttatcc
     4621 tgtgtagcaa tatagtgtta aattgccgtc aagataccga aacttcttat attacgcaat
     4681 tgcctaatgt gaaaaaaaca accgaaatta gcgtattttg ccgacatttt tacttaaaat
     4741 aacgacaaag tcagaatttt aggaaagcaa catgatacta aagcctttat tgatatttgg
     4801 tgtattatct tgcctaacaa tagggcaagt ttatgcagaa gaaagctcat cagtcgatgc
     4861 gatgatgcca aatttagtga ttgatttgtc gtcagttgct agtcaagtat ggtttgccca
     4921 agcacaaatt tggggggcgt gtagtttagt ggctttatta gtggcaattt ttgcgtggat
     4981 tttattgaat attaattcat taaaacaaca agataatcaa ggggatgtgt tagctaaacg
     5041 agtatcgtta ttgtgggtag cgtttttgat ggtgatggta gtgagttttg ccataccttt
     5101 gttgagagta tggctggtat tggcttaaat aactataggc aatgcctgtc agtaagtttt
     5161 tatatgagct tggtttcgac tccgctcaac cagcc
//
LOCUS       DAOQMB010000138         5194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7287_length_5194_cov_15.280016, whole genome shotgun sequence.
ACCESSION   DAOQMB010000138 DAOQMB010000000
VERSION     DAOQMB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7287_length_5194_cov_15.280016"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..492)
                     /locus_tag="PLY05_06055"
     CDS             complement(22..492)
                     /locus_tag="PLY05_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019454693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HNC02903.1"
                     /translation="MMNANDVLRSLRYTLDINETAVVGLLSLTGFQASTEQVSAWLAK
                     EDEANYQPCPELALAHFLDGLIIDCRGLPEGKDYQAPNPNIALSNNMVLKKLRIAFEL
                     QEDEMLETLDAAGLRVSKQELSALFRKEGHHNYRECGDQFLRNFLKGLNVRFRK"
     gene            complement(489..740)
                     /locus_tag="PLY05_06060"
     CDS             complement(489..740)
                     /locus_tag="PLY05_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004698872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HNC02904.1"
                     /translation="MKIIARNLPRALTEAQLKALFDEFGLVESCNLVLDKATGKSKGF
                     GFVEMPKHIEAKLAIKRLNAKKILDSVIRVKEAEEGAAK"
     gene            complement(788..>1405)
                     /locus_tag="PLY05_06065"
     CDS             complement(788..>1405)
                     /locus_tag="PLY05_06065"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HNC02905.1"
                     /translation="SDDDYDRQQWSRLWLVDPLDGTKEFVARTNDFTVNIALVVDHQA
                     VLGILHAPYYGLTYMAAKGCGAWLIDQHGEKQRLTTAKQLTPPRLLVSRFHANGKKNQ
                     ALLQHTQQRFGDYQVLEVGSALKMCRIAEGLADFYPRFGTTMEWDTAAGQIILEEAGG
                     ALVDRQGRPFLYNKRDSLANKDFIVVGDKAYVDDWLACMQVESAR"
     assembly_gap    1406..1505
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1506..2126
                     /gene="yrfG"
                     /locus_tag="PLY05_06070"
     CDS             <1506..2126
                     /gene="yrfG"
                     /locus_tag="PLY05_06070"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HNC02906.1"
                     /translation="DMDGTLLDLHFDNYFWQIHVPEQYSQKHGLSLEESKAVLYPRFA
                     AQQGSLNWYCLDYWSKDLGLDIVVLKHDIQERIALRTEALDFLQALAASHKQVWLVTN
                     AHPQALALKLNKTLISHFFDHIISSHQFGKPKENLQFWHDLQNYSHFVPEQTLFIDDS
                     ESVLKTAANFGIKHILSIKHPDSQTVQHRQSQFPMLEHFKDILPIA"
     gene            2263..2514
                     /locus_tag="PLY05_06075"
     CDS             2263..2514
                     /locus_tag="PLY05_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02907.1"
                     /translation="MSAALTLANLPEDLLSRLQEAAQQHGCSIEQEIESCLRKVLMPI
                     TTTEELISRTQELRQQFVCEADRLADVVRRLEEGKPCLL"
     gene            complement(2550..3212)
                     /locus_tag="PLY05_06080"
     CDS             complement(2550..3212)
                     /locus_tag="PLY05_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC02908.1"
                     /translation="MQNSDEMRTLVPKKTPKRGYGGRSAEQLAQERYERLMASALELF
                     GTVGYLPTTVEKLCAHAKVTPRHFYEHFKDREAILIAVFNDILQQSKHAVLSQMLNGE
                     LSLEQRFEAGVNAFLSAHLEDLRRVKITTQEILGVSQRVEAARNAAITEFALLIESYL
                     GLWVAAGKLPARNYKVLAFGLVGAMHELQIAWLNKQVPQEKAVLVEEMAFLMSCLVKG
                     VR"
     gene            3291..4070
                     /locus_tag="PLY05_06085"
     CDS             3291..4070
                     /locus_tag="PLY05_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC02909.1"
                     /translation="MSAKAIFITGAAAGIGAATARLFAKNGWFVGLFDIDQVGLAKLQ
                     QELGTQVSMTGVLNVADEQQWQQALHQFFSRTGRLDVLLNNAGILASGAFEHIPLQQQ
                     HRLLDVNIKGVLNGCYLAFPYLQQTAKSRVINLCSASALYGQPSLAVYSATKFAVRGL
                     TEALDLEWSAYNIRVMDVLPLFVQTNMVNNMDADSIKNMGVHLTAEDVAQTIYQAADY
                     QGKIAKVHWTVGLPTRLFYAVSGAAPDWLSRGINQFINRQK"
     gene            4161..>5194
                     /locus_tag="PLY05_06090"
     CDS             4161..>5194
                     /locus_tag="PLY05_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02910.1"
                     /translation="MSLQIPASMQRITDFDTTRQSPLPRERLEATRKAALAFRERFMD
                     EPEVLFYKSINLIRVPYPTWYGYSGVYAQSAYRFPFIHILNRLFVIQYRDFAGEVKTL
                     LFSPSDIDGNRETPFFKRLVEKLHLPKSAQDFMAPTYHTVESALASTGIRPEQVDYIS
                     YDHLHTQDLRKWLGTGANKGYFPNAKLLVHEQEWASTSALIPCQADWYCPHGIDGVNT
                     SRIIQFTGSIQLGRGVALVHTLGHTEGNHSLVARVKDGIRVTSENGVGADAYQPINST
                     INAIRRYAQATGAEVILNGNTLEGSNDQYISMVMEKTIAGASVNSDYPNCASSSEATP
                     YWLVPQQQSS"
BASE COUNT         1498 a         1142 c          917 g         1537 t
ORIGIN      
        1 ccccatctgc ttacaacaca actactttct aaaccttaca ttcaatccct tcaaaaagtt
       61 tcttaaaaac tgatcgccac actcacgata attatgatgg ccttcttttc taaacaaggc
      121 acttaactct tgtttagaca cacgcaaacc tgcggcatct aaagtttcga gcatttcgtc
      181 ttcttgtaac tcaaaagcaa tacgtaactt ttttaagacc atattattgc ttaatgctat
      241 gttgggatta ggtgcttgat agtctttgcc ttcaggtaaa ccacgacaat caataattaa
      301 gccatctaaa aagtgcgcta acgccaattc tggacaaggt tgataatttg cctcatcttc
      361 tttagccaac caagcagaga cttgctcagt gcttgcttga aagcctgtta atgacaataa
      421 acccaccaca gccgtttcat taatatcaag ggtataacgt aagctacgca acacatcatt
      481 agcattcatc attttgctgc accctcttcg gcttctttaa cccgaatcac actatccaaa
      541 atcttttttg cattcaaacg cttaattgct aattttgctt ctatgtgttt tggcatctca
      601 acaaaaccaa agcctttaga tttgccagtg gctttatcta aaaccaaatt acaagattct
      661 accaaaccaa attcatcaaa taaggcttta agttgtgctt cggtcaacgc acgtggcaaa
      721 ttacgggcaa taattttcat gtctattctc aaaaaactta gctaatcatc ataacatact
      781 tacaggttta gcgtgcgctc tcaacctgca tacaggccaa ccaatcatcg acataagctt
      841 tatcaccaac aacaataaaa tctttattgg ccaaactgtc acgtttgtta tataaaaatg
      901 gtcggccttg acgatctacc aaggcaccac ccgcctcttc taaaataatt tgtcctgccg
      961 cagtatccca ctccatggtt gtgccaaaac gcggataaaa atcggctaag ccctcagcaa
     1021 ttcgacacat ttttaaggca ctacccacct ctaacacttg ataatcacca aaacgttgct
     1081 gggtgtgttg taataaagct tgattttttt tgccattggc atgaaaacga ctgactaata
     1141 aacgaggcgg agtgagttgt ttggcagttg ttaaacgttg tttttcacca tgctgatcaa
     1201 tcagccatgc gccacagcct ttcgctgcca tatacgttaa gccataataa ggtgcatgta
     1261 atatccctaa taccgcttga tgatcaacca ctaaggcaat attgaccgta aagtcgttag
     1321 tacgtgccac aaattcctta gtgccatcca aaggatcaac caaccacaaa cgtgaccatt
     1381 gctgacgatc ataatcatca tctgannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnngatat ggatggtacg ttattagact tacattttga taattatttt tggcaaattc
     1561 acgttcctga acagtacagc caaaaacacg gactttcatt agaagaaagc aaggccgtat
     1621 tgtacccccg ttttgctgca caacaaggct ccttaaattg gtattgctta gattattggt
     1681 ctaaagattt aggcttagat attgttgttc taaagcatga tatccaagaa cgaattgccc
     1741 tacgcaccga agctctagac tttttacaag cattggctgc cagtcacaaa caagtttggc
     1801 tagtaactaa tgcccatccg caagccttag cactcaaact caataaaacc ttaattagtc
     1861 atttttttga tcatattatt agctcacacc agtttggtaa acccaaagaa aacctgcaat
     1921 tttggcatga cctacaaaac tactcacatt tcgtccctga acaaacgtta tttattgatg
     1981 acagtgagtc ggtgttaaaa acagcggcaa attttggcat taaacatatt ttgtcaatta
     2041 agcatcctga tagtcaaacc gtgcaacatc ggcaatcaca attccctatg ctcgagcatt
     2101 ttaaagatat tttgccaatt gcttagcttt tatgatgatt gataaaaatt gtctgacaat
     2161 atggtttgac agaccttgct catctagcga aaatcgcaaa ggtcttatcg ttcctatcaa
     2221 atcaaaacag tactttcgta ctaatttact tgagagtcac acatgtctgc tgctttaact
     2281 ttagctaatc tccccgaaga tttattaagt cgtttgcagg aagcagctca acaacatgga
     2341 tgcagtattg aacaagaaat tgaatcttgt ttgcgtaagg tgctaatgcc cattaccacg
     2401 actgaagaat taattagtcg tactcaagaa ctccgtcagc aatttgtttg tgaagccgat
     2461 cgtttggctg acgtcgtccg ccgcttagaa gaaggcaaac cctgtttgtt ataaatttta
     2521 tttggactag gttcgttcct agtccaactt taacgcacac ctttcaccaa acaactcatt
     2581 aaaaaagcca tttcctctac caacacagct ttttcttgag gtacttgttt gtttaaccat
     2641 gcaatttgta attcgtgcat cgctcccact aagccaaaag ccaagacttt gtagttacga
     2701 gctggcaact tgcctgcggc aacccacaga cccaaatagc tttcaattaa tagcgcaaat
     2761 tctgtaatag ctgcatttct ggctgcttca acacgttggc tcacccctaa aatttcttgt
     2821 gtggtaattt ttacccgacg cagatcttct aaatgtgcac ttaaaaaggc attaacacct
     2881 gcctcaaaac gttgttctaa agataattca ccattcaaca tttgactcaa tacagcatgt
     2941 ttgctttgtt gcaaaatgtc attaaacaca gcaattaaaa tcgcctcacg atctttaaaa
     3001 tgctcataaa aatgacgtgg tgtgactttg gcatgagcac ataatttttc gaccgttgtt
     3061 ggcaaatatc ccactgtacc aaacaattcc aaagcacttg ccattaatcg ctcatagcgc
     3121 tcttgagcca attgctcagc actacgccca ccataaccac gtttaggcgt tttttttggt
     3181 actaaagtac gcatttcgtc ggaattttgc atattgcccc ttgaattagg aaagacttct
     3241 ttcctaaaat aactgcatta tatatgaata aatttttctg ggggtattat atgtcagcta
     3301 aagctatttt tattaccggt gcggccgctg gcattggtgc cgccactgct cgtttatttg
     3361 ccaaaaatgg gtggtttgtt ggcttatttg atattgatca agttggtttg gccaaattac
     3421 aacaagagtt gggtacacag gtgtcaatga caggtgtgct taatgtcgct gatgagcaac
     3481 aatggcaaca agccttgcat caatttttta gcagaacagg ccgattggat gttttgttaa
     3541 ataatgctgg gattttagcc tctggagcat ttgaacatat tcccctacaa caacaacatc
     3601 gtttattaga cgttaatatt aaaggggtac tcaacggctg ctatttagct tttccttatc
     3661 tacaacaaac agccaaaagc cgtgtcatca acttatgttc agcatctgcc ttgtatggcc
     3721 aaccctcttt agctgtttat tcagcgacta aatttgcggt gcgtggttta accgaagcct
     3781 tagatttgga gtggtcggct tataatattc gtgtaatgga tgttttgccg ttatttgtac
     3841 aaaccaacat ggttaataac atggatgctg acagtattaa aaatatgggc gtgcacttaa
     3901 ctgccgaaga tgttgcccaa actatttatc aggcagcgga ttatcaaggc aaaatcgcca
     3961 aagtccattg gacggttggc ttacctactc gcttatttta tgccgtgtca ggtgctgcgc
     4021 ctgattggtt aagccgtggc attaatcagt ttattaatcg tcaaaaatag gcttcgacga
     4081 cgctcagcca actaaataac ttaacgcact aaccctagct ctctcctccg aatgataggg
     4141 agcttttcaa ggatacaatt atgagcctac aaattcctgc ctccatgcaa cgcatcaccg
     4201 attttgatac tactcgtcaa tcacctttac cccgcgaacg cttagaagcc actcgtaaag
     4261 ccgctttagc ctttcgtgag cgttttatgg atgaacccga agtcttattt tataaaagta
     4321 ttaatttgat tcgtgtgccc tatcccacat ggtatggcta tagtggggtc tatgcgcaaa
     4381 gtgcgtatcg ctttccattt attcacattt taaatcgctt gtttgttatt cagtatcgcg
     4441 actttgcagg tgaagttaaa actctattgt tttccccatc tgatattgat ggcaatcgcg
     4501 aaaccccatt ttttaaacgt ttggttgaaa aattacattt gcctaaatca gctcaagatt
     4561 ttatggcacc aacctatcat acggtagaaa gtgctttggc cagcacaggt attcgtccag
     4621 aacaagtgga ctatattagt tatgaccact tacataccca agacttgcgc aaatggctag
     4681 gaacaggcgc aaacaaaggc tattttccca atgctaaatt attggtgcat gagcaagaat
     4741 gggcatcaac cagcgcatta attccttgcc aagcggattg gtattgccct catggcattg
     4801 atggtgtgaa tacaagtcga attattcaat ttacaggcag tattcaactc ggccgtggcg
     4861 tggccttagt acataccttg ggacacaccg aaggcaatca ttcgttagta gcacgtgtta
     4921 aagatggtat tcgtgtcacc tcagaaaacg gtgtgggtgc ggatgcttat cagccaatta
     4981 attcaacgat taatgctatt cgtcgttatg cccaagcaac aggtgcagaa gttatcttaa
     5041 atggtaatac cttagaaggc agtaatgacc aatatatttc aatggtcatg gaaaaaacca
     5101 tcgctggagc atcggttaat tccgattatc ccaattgtgc ctcctcaagc gaggcaacac
     5161 cttattggct agtaccacaa caacaaagta gctt
//
LOCUS       DAOQMB010000139         5191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7291_length_5191_cov_15.952492, whole genome shotgun sequence.
ACCESSION   DAOQMB010000139 DAOQMB010000000
VERSION     DAOQMB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7291_length_5191_cov_15.952492"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..776
                     /locus_tag="PLY05_06095"
     CDS             21..776
                     /locus_tag="PLY05_06095"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC02911.1"
                     /translation="MKLPHIWQPDARLDNRHQLADWFGSPLGQEVLSAQQQILSHILP
                     DFFGYHALQLGQVVPCHLLQHSRIAHRIVADKHLATIHGLSPLLALPEQLPLATNSMD
                     VVLIHHLLDIAKSPHAVLREACRVVIPDGYLIIVGFNPWSLWGLWRFCRLPWSNTPWL
                     RRSLSAQRLADWLTLLDFDVVGVESAYFKPPIAQDTLRQYFQWLELVGAKYWSHGGAS
                     YIILAQKKESCMTPIRLRKPLLPLMPAPLLAEN"
     gene            785..1938
                     /gene="dnaQ"
                     /locus_tag="PLY05_06100"
                     /pseudo
     CDS             785..1938
                     /gene="dnaQ"
                     /locus_tag="PLY05_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005246882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
     gene            2143..3819
                     /locus_tag="PLY05_06105"
     CDS             2143..3819
                     /locus_tag="PLY05_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02912.1"
                     /translation="MLPRIDVTALNLIKTEVDASLTQIEGALSTFVEDNANNGPLAES
                     VEQMEQVYGALRLIDIHGAAELAEVLFALLRQVNDLQEKTPDIYYSALGSGMMVLGRY
                     LEYVQIKAVNLPQLLISSINECRYALGLPTLGEGYFLDVPFLPDSNIVEPLAIDNSQA
                     VALAKRIRLMYQIGTINLLKDEAQPVHFRLMRRALERATQLCTGKPLALLWWVGEAAL
                     EAFSQGVELNPARKRLLAQLEQQLKLLVLPQGLNQPVDMAVLANSLAVVGLGETGAKV
                     QEVQRAFDLSSSCISQSQLKAQQEAMYGPGGSVIKTVATVLKEDIARIKEVLDVIARG
                     SQQEEESYQTVSDAILKSSQTLIMLGLNDAANSLRAQANIVAQWTDTPTEAALNLLFD
                     VLLQADNAVATLDKEMSPGAQTIVNNTRISLHQLDEARGMLVQESRSGIELVKRSVLS
                     YLEGNGEDPMHLSNIPTTLQSVSGGLEFLLIPRGAAILRAAANFVDAWALSKKMPAMS
                     IIENFADVIACVDYYLESLQENKPVSEEVFTIGEDAMAELGYPIAKRKAA"
     assembly_gap    3878..3977
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4248..>5191
                     /gene="sohB"
                     /locus_tag="PLY05_06110"
     CDS             4248..>5191
                     /gene="sohB"
                     /locus_tag="PLY05_06110"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008745.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HNC02913.1"
                     /translation="MIDLLLQYGLFLAKVVTVVVAALFIIVTIANVGERRRHHDEDGD
                     IEVMPLNERLEHFANTLRMAVLDKNALKAYFKSQKDQEKSKEKQTNDDKKRVYVINFN
                     GDIRASATENLRQEITAILSIANKDKDEVVVNLESPGGMVHGYGLAASQLARLRDHQL
                     PLTICVDKVAASGGYMMACLANTIIAAPFAVIGSIGVVAQLPNFHRLLDKWDIDYEVL
                     TAGEYKRTLTMFGKNTDAGREKFIADLEDTHNLFKEFVTDYRPQLDIEKVATGEHWYG
                     KQALGLGLVDKLMTSDEYLVSLSKTADIFAVRFIHKQN"
BASE COUNT         1559 a          825 c         1123 g         1584 t
ORIGIN      
        1 acagcgatat tttttaacat atgaaactac ctcatatttg gcaacctgat gcccgtttag
       61 ataatagaca tcaattagcg gattggtttg ggagtccgtt aggacaagaa gttttgtctg
      121 ctcaacaaca gatattaagt catattttac ctgatttttt tgggtatcat gccttgcaat
      181 tggggcaagt cgtgccttgt catttattac aacacagtcg tattgcgcat cgtatcgttg
      241 ccgataaaca tttagcgact attcacggtc tatcaccatt attagcctta ccagagcaat
      301 tacctttagc aacaaatagt atggatgtgg tactcattca ccatttatta gatattgcca
      361 aaagtcctca tgcggtgtta cgcgaggctt gtcgagtggt tattcctgat ggctatttga
      421 ttattgttgg ttttaatccg tggagtttat ggggattgtg gcgtttttgt cgcttgccgt
      481 ggtctaatac accatggcta agacgttctt taagtgcgca acgtttggcc gattggctaa
      541 ccttattaga ttttgatgta gtaggggtag agtctgccta ttttaaacca cctatagcgc
      601 aagatacttt acgtcaatat ttccaatggt tagagcttgt gggcgcaaaa tattggtctc
      661 atggtggagc aagttatatt atcttggcac aaaaaaaaga atcttgtatg actcctatac
      721 gcctacgtaa acccttattg ccattaatgc cagccccatt attagcagaa aattgagtga
      781 atatatgcaa aaaatagaga tttataccga tggtgcgtgt cgaggtaatc caggtattgg
      841 tggttgggga gcattgttga tttatcaagg tacagaaaaa cgcctatacg gtagtgaaga
      901 aaacaccact aataatcgta tggaattgat gggcagtatt gaagccttaa aagctctaaa
      961 aagaccctgt gaagtcatct tatggacaga ctcacaatat gtcaaaaaag gcattagcga
     1021 atggttagca ggttggaagg caaaaggttg gcgtacagcc agtaatcaac ccgtaaaaaa
     1081 ccaagattta tggcaaatgt tagatcaaga agctaatcgt catcacatag attggcgatg
     1141 ggtgaaaggt cacgcaggtg aatatggtaa tgaagttgca gaccaattgg ctaatcaggc
     1201 aatagatgaa ttgttaggta aataaagatg agacaaattg tattagatac tgaaaccaca
     1261 ggtattagtc ctagtgatgg ccatcggatt attgaaattg gttgtttaga attagaaaat
     1321 cggcgtttaa ctggccgtag ttttcaccac tatttgcaac ctgatcgtga aatagaacaa
     1381 gaagcgattg cagtacatgg tattactaat gaatttttaa ttggtaagcc tagattttct
     1441 gatgttgctc aagagtttat gaagtttatt tcaggtgcag aattaattat tcataatgca
     1501 ccgtttgata ttggttttat caatcatgaa tttaaacttc tagataatag tttttctacg
     1561 attgtgggta tttgtcaggt tttagatacc ttagtgttgg cacgacaaaa acatccagcg
     1621 caaaaaaata atttggatgc attagctaga cgttatggtg cagatcatcg tgagcgcact
     1681 tatcatggtg cattattaga tgctgaaata ttagccgatg tatatttggc catgacaggt
     1741 ggtcaagtag gtttgagttt gcacgatgat gcagatggtg agggtggcca acaagaagtc
     1801 attcaacgtt tatctgcaaa tcgtttgcca ttaaaagtca ttaaggctaa tgagcaagaa
     1861 ttgagcgaac atgaagctcg tttaggtatt attgataaag cttgtggtgg aagttgtgta
     1921 tggcgtcagt tggcttaaat aaataaagat ggtataagta cactccaagt gaaacaatcc
     1981 ataatgctga gagttaattt aggtctaaaa ttgtgaaata gttaacaata ataaagaact
     2041 atggtacaaa ttagactagc ttagaatcca taaaagcatt atttttgtta taaatttttt
     2101 taaacagacc ctattaaaat caaacgatac aggaaggaac ccatgcttcc aagaatagat
     2161 gtaaccgcat taaatttaat taagaccgaa gttgatgcct ctttaacaca aatagagggt
     2221 gcgcttagta cttttgttga agataacgct aataatgggc ctttagcaga gtctgtagag
     2281 caaatggagc aagtctatgg cgcgttacgc ctaatagata ttcatggtgc tgctgaactt
     2341 gctgaagtat tatttgcatt attgcgccaa gtaaatgact tacaagaaaa aacacctgat
     2401 atttactatt ctgctttagg cagtggcatg atggtattgg ggcgttattt agaatatgtg
     2461 caaattaaag cggtgaattt gccacaatta ttaatttctt caattaatga atgtcgttat
     2521 gctttaggtt taccgacttt aggtgaaggt tatttcttag atgtaccttt tttacctgat
     2581 agtaacatcg ttgaaccctt agcaatagat aatagtcaag cagttgcttt ggcaaaacgt
     2641 attcgtttga tgtatcaaat cggtacaatt aatttattaa aagatgaagc ccaacctgtt
     2701 cattttcgct taatgcgccg cgctttggaa cgtgcaactc aattatgcac aggcaagcct
     2761 ttagctttat tgtggtgggt gggagaagca gcactagaag ctttttctca aggtgtggag
     2821 ttaaacccag cgcgtaaacg tttattagcg cagttggagc aacaattaaa gttattggtt
     2881 ttgccacaag gacttaatca gcctgtagat atggcagtat tggctaatag cttagcagtg
     2941 gttggtttgg gtgaaacagg tgctaaagtt caagaagtac aaagagcttt tgatttatcc
     3001 agttcttgta ttagccaaag tcaattaaag gcgcagcagg aagcgatgta tgggcctggt
     3061 gggtcagtca ttaaaactgt tgctactgtc ttaaaagaag atattgctcg tatcaaagaa
     3121 gtattagatg taatcgctcg tggctctcaa caagaggaag aaagctacca aactgtttct
     3181 gatgcaatcc taaaatcttc tcaaacgctg attatgttag gtttaaacga tgcagcaaat
     3241 tctttgcgcg cacaggcaaa tattgtcgca caatggacgg acactcccac agaggccgca
     3301 ttaaatctat tattcgatgt gttgttacaa gcagataatg ctgttgcaac actagataaa
     3361 gaaatgtctc ctggagcgca aactattgtt aataatactc gtatttctct ccatcaatta
     3421 gacgaagcac gcggtatgtt ggtccaggaa agtcgctcag gtattgaatt agtcaaacga
     3481 tcagtgttgt cttatttgga aggtaatggt gaagatccca tgcacttatc aaatattcca
     3541 acaaccttac aatcagtgag tggcggttta gagtttttgt taattccacg tggtgctgct
     3601 attttacgtg ctgccgctaa ttttgtggat gcatgggcgt taagtaaaaa aatgccagca
     3661 atgagtatta ttgaaaactt tgctgatgtg attgcttgtg tggactatta tttagaaagt
     3721 ttacaagaaa ataaacctgt cagcgaagaa gtgtttacta ttggcgaaga tgcgatggcc
     3781 gaattgggtt atcccattgc taagagaaaa gctgcttaat gacaagctca aacatacagc
     3841 cagaactgac cctctggctg ttatttaaag gtcaagannn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnngag tgggcaggtg gtgatattgt gttacaagag gaagaaatta
     4021 tggatgcaca attttttcct tatgatgatt taccagaagt tccacccgca ggcagtattg
     4081 ccacacaagt cattaatgcc actgttgctg aattagcaca gcgttatcca cgtatctagt
     4141 cgccaataaa agttggtgtt tttgtggttg cacacaaaac gccaacaaat tttatctgct
     4201 ttctgtttaa aatcgtcaga atttaatgtg tttctaggat catctgcatg attgatttac
     4261 tgttacagta tggattgttt ttggccaaag tcgtgacggt tgttgttgct gctttgttta
     4321 tcattgtcac tattgccaat gttggcgagc gtcgtcgtca tcatgatgaa gatggtgata
     4381 ttgaggtgat gcctttaaat gagcgtttag aacattttgc taatacttta cggatggcgg
     4441 tattagataa aaatgcctta aaagcctatt ttaaaagcca aaaagatcaa gaaaaaagca
     4501 aagaaaagca aacaaatgac gataagaaac gcgtgtatgt gattaatttt aatggtgata
     4561 ttcgtgcttc tgcaactgag aatttacgtc aagaaattac tgcaatttta agtattgcca
     4621 ataaagataa agatgaagtt gttgtcaatt tagaaagccc tggtggtatg gtgcatggtt
     4681 atggtttagc cgcatcacaa ctggcaagac tcagagacca tcaattaccg ctaaccattt
     4741 gtgtcgataa agtagccgct agtggtggtt atatgatggc ttgtttagcc aacaccatta
     4801 ttgctgcacc ttttgcggtg attggttcga tcggtgttgt ggctcagtta ccaaactttc
     4861 atcgtttatt agataagtgg gatattgatt acgaagtctt aaccgcaggt gaatataaac
     4921 gtactttaac catgtttggt aaaaatactg atgcaggtcg tgagaaattt atcgcggact
     4981 tagaagatac ccataacctt ttcaaagagt ttgtgactga ctatcgtcca caattggata
     5041 ttgaaaaagt ggcaacaggt gagcattggt atggtaaaca agctctaggt ttgggtttgg
     5101 tcgataaatt aatgaccagt gatgaatatt tagtgagttt aagtaaaaca gctgatattt
     5161 ttgcagtacg ttttattcat aaacaaaact t
//
LOCUS       DAOQMB010000140         5136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7434_length_5136_cov_14.265696, whole genome shotgun sequence.
ACCESSION   DAOQMB010000140 DAOQMB010000000
VERSION     DAOQMB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7434_length_5136_cov_14.265696"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2598
                     /gene="topA"
                     /locus_tag="PLY05_06115"
     CDS             <1..2598
                     /gene="topA"
                     /locus_tag="PLY05_06115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HNC02914.1"
                     /translation="IVESPAKAKTINKYLGKDFIVKSSIGHVRDLPVSGSGKDEKEEK
                     EAKTTTRARLTAEEKATKARSALFARMGVDPEHDWLANYQILPGKEKVVAELKSLANS
                     VETVYLATDLDREGEAIAWHLQQVIGGDPNRYQRVVFNEITKTAITAAFKQPARLNLD
                     RVNAQQARRFLDRVVGYMVSPLLWEKIARGLSAGRVQSVAVRLVVEKEREIRAFVPEE
                     FWDIHADTQTQGKAALGLQVSKYKEQTFKAKNEQEAKTAVAHLQKANYVVSKREDKPT
                     KTRPPAPFITSTLQQAASTRLGFSVKKTMTLAQRLYEAGYITYMRTDSTNLSQDAIQA
                     VRGFISHEFGNQYLPAKANIYASKSGAQEAHEAIRPSDVNAKPSSISGLERDAERLYE
                     LIWRQFVACQMPEAEYMSTSLAVAAGDYELRTRGRILTFDGFTKVLSAVQKENDDVVL
                     PNVKVGEALTLLKLLPTQHFTKAPPRYTEATLVRELEKRGIGRPSTYASIISTIQDRG
                     YVKLDSKRFYAEKMGDIVADRLLESFDNLMDYAFTAKLEEELDKVAEGDVNWKKLLND
                     FYSDFKAKLEKASGENGMRRNQPVEIGVPCHLCSRPMQVRTASTGVFLGCSGYALPPK
                     ERCKGTVNLTPVEHLALADNDEEGETNELRAKKRCPKCSTAMDSYLVDAERKLHVCGN
                     NPDCNGFVLEQGSYKLKGYEGPTVECDKCGGSMELKTGRFGAYFACSSCPNTRKVLKN
                     GEAAPPKAEPIHTDIKCSKVSDYFVLRDGAAGLFLAASQFPKNRETRTPSIAELKQVA
                     DRLDPKYHYLLSAPATDDYGNPSLLRFSRKTKEQFVATEEDGKPTGWQAFYRDGSWKI
                     TKGKMSKEK"
     gene            2675..3175
                     /locus_tag="PLY05_06120"
     CDS             2675..3175
                     /locus_tag="PLY05_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004710685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02915.1"
                     /translation="MRVARYGAGRTNQKFYFAKMAMQQIEQAVDEPSKQANYEAAVFH
                     LHGAFLAFLQELSRFYKLSPQLGTTEAIRLALDAKGQVSPEISQLQAVEDGGWLGELQ
                     QAFADCQLAPEPVEVQPEAELAEGLIMRAVTNPVEPEPVEQRLKAWHTQLQRLFSEFR
                     QEMVEF"
     gene            3175..3474
                     /locus_tag="PLY05_06125"
     CDS             3175..3474
                     /locus_tag="PLY05_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02916.1"
                     /translation="MPEAMLEIVELDDGDVVLRRVDSAEGSSEPFVRIHFSEEAKGLI
                     NGQSAQLGRLMISMGLQAVAKAHAEALKEAIESGELSDVDIETMEEAPPPNTKLH"
     gene            3839..4984
                     /locus_tag="PLY05_06130"
     CDS             3839..4984
                     /locus_tag="PLY05_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC02917.1"
                     /translation="MSLYTAYFNETHNQVRLTARKFVDQHIRPFINDWEEAGTFPREL
                     YNLAGEAGLLGIDHPEELGGSGTDIFMKIAASEEIMRAGSGGLAASLGSLDIGLPPIE
                     NWASQELKERIVPAVLRGEKIAALAITEPSGGSDVANLKTRAESDGDYYRVNGSKTFI
                     TSGHRADYYTVAVRTGGEGYGGISLLLIERDRAGFSVGQPLKKMGWWASDTAELFFND
                     VLVPKSNIIGAENAGFFVIMSNFQKERLNLAVMANMTAQLAVEECLKYAQQRVAFGKP
                     LAKMQVIRHKLADMATDLEASKEFTYRVAVQIASGIPAVKEVSMAKNFATRVSDSVTY
                     EAVQIFGGMGFMRESLVERLYRDNRILSIGGGTHEIMNEVIAKQLGL"
BASE COUNT         1552 a          948 c         1202 g         1434 t
ORIGIN      
        1 attgtggagt cgccagcgaa ggcgaaaacc attaataaat atttagggaa agattttatt
       61 gttaaatcaa gtattggtca tgtgcgtgat ttacccgtca gtggtagtgg caaagacgaa
      121 aaagaagaga aagaggccaa aacaaccaca agagcacgtt tgactgccga agaaaaagca
      181 acaaaagcac gcagtgcctt atttgctcgt atgggcgttg atcctgagca tgattggtta
      241 gctaactacc aaatcttacc cggtaaagaa aaggtagtcg ctgaattaaa atcgctcgcc
      301 aattcggtag aaaccgttta tctagccacc gacttggata gagagggaga ggcgattgct
      361 tggcatttgc aacaagtgat tggtggtgac ccaaatcgtt atcaacgtgt tgtatttaac
      421 gaaatcacca aaactgcgat tactgccgct tttaaacagc ctgcaagatt aaatttagat
      481 cgtgtgaatg cccaacaagc ccgccgcttt ttagatcgtg ttgtgggtta tatggtctcg
      541 ccattgttgt gggaaaaaat tgctcgcggt ttatcggcag gacgtgtgca atcggtggcg
      601 gtgcgtttag tggttgaaaa agagcgtgaa attcgtgctt ttgtgcctga agagttttgg
      661 gatattcatg ctgacaccca aactcaaggc aaagccgctt taggtttgca agttagcaaa
      721 tataaagagc aaacgtttaa ggccaaaaat gagcaagaag caaaaaccgc tgtggctcat
      781 ttgcaaaaag ccaattatgt ggttagcaaa cgtgaagata aaccgaccaa aacacgacca
      841 ccagcaccat ttattacctc aactttacaa caagcagcga gtacacgttt aggttttagt
      901 gttaaaaaaa ccatgacctt agcccagcgt ttgtatgaag caggttatat tacttatatg
      961 cgtaccgact caaccaattt gagccaagat gctattcaag cggtacgtgg ttttattagt
     1021 catgagtttg gcaatcaata tttacctgcc aaagctaata tttatgctag caaaagtggc
     1081 gcacaagagg ctcacgaagc gattcgacct tcagatgtta acgcaaaacc cagtagtatt
     1141 agtggtttag aacgtgatgc tgaacggtta tatgagttaa tttggcgtca atttgttgcg
     1201 tgtcaaatgc ctgaagccga atacatgagc acttcattag ccgtagccgc aggtgattat
     1261 gaattgcgta cacgcggtcg tattttgacg tttgatggct ttaccaaagt attgtcagcg
     1321 gtacaaaaag agaatgatga tgtggtcttg cctaatgtca aagtgggtga agccttaaca
     1381 ttactcaaat tgttaccaac acagcatttt accaaagcac caccacgcta caccgaagca
     1441 actttagttc gggagttaga aaagcgtggc attggccgcc catcaaccta tgcgtcaatt
     1501 atttcgacca ttcaagaccg tggctacgtt aaattagata gcaaacgttt ttatgccgaa
     1561 aaaatgggtg atattgttgc tgatcgtttg ttagaaagct ttgataactt aatggattat
     1621 gcgtttaccg ctaagttaga agaagagtta gataaagtcg ctgaaggtga tgttaattgg
     1681 aaaaagttgc tgaatgattt ttatagcgac tttaaggcca aactcgaaaa agcctctggc
     1741 gaaaatggta tgcgccgcaa tcaacctgtc gagattggtg tcccttgcca tttatgttca
     1801 cgtcctatgc aagtacgtac tgccagcaca ggcgtctttt taggttgtag tggttatgct
     1861 ttaccaccta aagagcgttg taaaggcacg gtcaatctca cacccgttga acatttagcc
     1921 cttgctgata atgatgaaga aggcgaaacc aacgaattac gcgccaaaaa acgttgtcca
     1981 aaatgcagca cggcaatgga tagctattta gttgatgctg agcgtaaatt acacgtttgt
     2041 ggtaataatc ctgattgtaa tggttttgtt ttagaacaag gcagttataa gctaaaaggt
     2101 tatgaagggc caacggtaga gtgtgacaaa tgtggcggct ctatggagct aaaaactggc
     2161 cgttttgggg cttattttgc gtgtagttca tgtccaaata ctcgtaaagt cttgaaaaat
     2221 ggcgaagctg ccccacctaa agcagaacca attcacaccg acattaaatg tagcaaggtg
     2281 agtgattatt ttgtgttgcg tgatggtgca gcagggttgt ttttggcagc tagccaattc
     2341 cctaaaaacc gtgaaacacg cacaccaagt attgctgaat taaagcaagt tgctgaccgt
     2401 ttagacccta aatatcatta tttattgtct gcacctgcaa cggatgatta tggcaaccca
     2461 agtttgttac gttttagccg caaaaccaaa gagcaatttg ttgcgactga agaagatggc
     2521 aagcctacag gttggcaagc attttatcgt gatggtagtt ggaagattac caaaggtaaa
     2581 atgtcaaaag agaagtagcg ttttaccctc actctaactc tctccctgcg ggagagggtt
     2641 ggggtgaggg cgtcaatcaa taaggtaaat atctatgcgc gtagcacgat atggtgcagg
     2701 ccgaaccaac caaaagtttt attttgctaa aatggcaatg caacaaatag aacaagcggt
     2761 tgatgaacct agcaaacaag cgaattacga agcagctgtg tttcatttac acggtgcgtt
     2821 tttagccttt ttgcaagaat taagtcgatt ttataaatta tcaccgcagc taggcacaac
     2881 cgaagcaatt cgtttggcat tagatgcaaa aggtcaagtg tcacccgaaa ttagccaatt
     2941 acaagctgtg gaagatggtg gttggttggg ggagttacaa caagcttttg ctgattgcca
     3001 attagctcca gaacctgttg aggttcaacc tgaagctgaa ttagcggaag gcttaattat
     3061 gcgtgcggtg acaaatcccg ttgaaccaga acctgttgaa caacgtctga aagcgtggca
     3121 cacgcaattg cagcgtttgt tttctgagtt tagacaagaa atggtagagt tttaatgcca
     3181 gaagcgatgt tagaaatagt tgaattagac gatggtgatg tggtgttacg tcgtgttgat
     3241 agtgctgaag gttctagcga gccttttgtg cgtattcatt tttctgaaga agccaaaggt
     3301 ttaatcaacg gtcagtcagc acaattaggg cgtttaatga ttagtatggg cttacaagcc
     3361 gttgctaaag cacatgctga agccttaaaa gaagccattg aaagtggcga gttatccgat
     3421 gtagatattg aaacaatgga agaagctcca ccacccaata ctaagttgca ttaaggggtt
     3481 atcgatctta cgattatgca catgataacc cccaaataac tagacaaatt taccaattct
     3541 aggcatgtgt tttttaacac atatcaccgt catatataca cgtatatttc tcattttcaa
     3601 atgatagaaa aataaccaag caaacgcttg gttgtttttt taaaatatgc cagtattgct
     3661 gatagcacat taggacttgc gctataccac tgcttgtgac ttacgcggtt atcgcttatt
     3721 tgcgcacatc atgggcattt ttagtgcctt tatgttcaca aaacgagcta atttcaacaa
     3781 agcgtaaatc cagaattcct agcgaaactt agttaactat cttacagagc aaaatatcat
     3841 gtcgctatac actgcttatt ttaatgaaac ccataaccaa gtacgtttaa ctgcccgtaa
     3901 atttgttgac caacatatta ggccgtttat taatgactgg gaagaagcgg ggacttttcc
     3961 gcgtgaatta tataacttgg caggtgaagc aggattatta gggattgacc acccagaaga
     4021 gttaggcggt agtggcacag atatttttat gaaaattgcg gccagtgaag aaattatgag
     4081 agctggatca ggtggtttgg cagccagttt aggttcgttg gatattggtt tgccacctat
     4141 cgaaaattgg gctagccaag aactaaaaga acgaattgta cctgcggtat tgcgtggtga
     4201 aaaaattgcg gcattagcca ttactgagcc gtcaggtgga tcagacgtcg ccaatctaaa
     4261 aactcgtgca gagagtgacg gtgattatta tcgggtcaat ggcagtaaaa cctttattac
     4321 ttctggacat cgtgctgact actatacagt ggctgtgcgt acaggtggtg aaggttatgg
     4381 cggcattagt ttgttgctta ttgagcgaga tagagcaggg ttttcagtcg gccaaccttt
     4441 aaaaaagatg gggtggtggg cgagtgatac cgcagaatta ttttttaatg atgttttagt
     4501 gccaaaatcg aatattattg gtgctgaaaa cgctggattc tttgttatta tgagcaattt
     4561 ccagaaagaa cggttaaact tggccgtcat ggcaaatatg accgcacagt tggcagtcga
     4621 agaatgttta aaatatgccc aacaacgtgt tgcctttggt aagcctttag ccaaaatgca
     4681 agtcattcgt cataagttag cggatatggc aaccgattta gaagccagta aagaatttac
     4741 ttaccgtgtt gcggtacaaa ttgcctcggg gattcctgcg gttaaagaag tgtcgatggc
     4801 aaaaaacttt gctacacggg tcagtgattc tgtcacctat gaagccgtac aaatttttgg
     4861 tggcatgggt tttatgcgcg aaagtctggt tgaacgttta taccgtgata atcgaatttt
     4921 atctattggt ggcggtactc acgaaattat gaatgaggta atcgccaaac aactgggatt
     4981 gtgatgagct ggcatttaat aaccgaacaa caacgattta aagagttatt aacaggatgg
     5041 gcgagtgcta aaacacttgc cttagatacc gagtttatgc gggttaatac ctttaatccg
     5101 caaattgctt tgttacaact caatgttaat caaaaa
//
LOCUS       DAOQMB010000141         5115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7490_length_5115_cov_15.507115, whole genome shotgun sequence.
ACCESSION   DAOQMB010000141 DAOQMB010000000
VERSION     DAOQMB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7490_length_5115_cov_15.507115"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..>1406)
                     /locus_tag="PLY05_06135"
     CDS             complement(66..>1406)
                     /locus_tag="PLY05_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HNC02918.1"
                     /translation="TGFDIIFFWVARMMMMTMHFMKNEDGTPQIPFKTVYVHGLVRDA
                     DGQKMSKSKGNVLDPLDIIDGIDLESLVGKRTTGLMKPQDAPKIEKNTRKEYPEGINA
                     YGTDALRFTFASLASTGRDINWDMKRLEGYRNFCNKLWNAARFVLSNCEGQDVAVDHD
                     NVELSVADKWIISRLQQAEQNVINALDSFRFDQAAQAIYEFVWNEYCDWYLELTKPVL
                     NGDSSPAAKAGTRRTLLRVLETMLRLMHPIMPYITEEIWQRVKGLVGKTGDSIMLQAY
                     PQPDLSKIDAQAEADVEWIKGVIAGIRNIRGEMNISPSKPLPILCHNVSAADATRLQA
                     YESQLRFLARLDSLTLLAADAELPPAATALVGDMAVLVPLKGFIDKDAEAARLNKEIA
                     RLEQEVTRIGGKLSNESFTAKAPVAVIAQEQKKLNDAKTAQAKLQEQLEKLKNL"
     assembly_gap    1407..1506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1507..2762)
                     /gene="valS"
                     /locus_tag="PLY05_06140"
     CDS             complement(<1507..2762)
                     /gene="valS"
                     /locus_tag="PLY05_06140"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNC02919.1"
                     /translation="MTLDATFNPKDSETRWYQQWETNGYFAPSGQGTPYCIMIPPPNV
                     TGSLHMGHGFNNTIMDSLIRYRRMQGYNTLWQPGTDHAGIATQMVVERQLAAQNISRH
                     DLGRDKFLEKVWQWKEQSGGTITRQIRRLGSSVDWTRERFTMDEGLSNAVKEAFVRLY
                     QDGLIYRGKRLVNWDPKLHTAISDLEVISEEENGSLWHFKYFLEDGSNHLVVATTRPE
                     TLLGDTAVAVNPEDDRYQALIGKKVRLPITGRLVPIVADSYVDKEFGTGCVKITPAHD
                     FNDFQLGKRHDLPLINIFDKDANILANFEVMAAGTFADLGQEAAPSEYAGLERFVARK
                     KLVKEAEENGWLDKIEPYKLKAPRGDRSGVIVEPWLTDQWYVKASVLAKPAIEAVEDG
                     RVKFVPEQYQNMYFAWMRDIQDWCISR"
     assembly_gap    3015..3114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3115..4235)
                     /locus_tag="PLY05_06145"
     CDS             complement(<3115..4235)
                     /locus_tag="PLY05_06145"
                     /inference="COORDINATES: protein motif:HMM:NF008737.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HNC02920.1"
                     /translation="MSRYLSTGSEWSFPLIAEYDAAIAEIAHGEFGIDTYPNQIEVIS
                     AEQMMDAYASVGMPIGYHHWSFGKHFVSTENAYQRGQMGLAYEIVINSNPCIAYLMEE
                     NTMTMQALVIAHACYGHNSFFKGNYLFKTWTDASSIIDYLVFARNYIRKCEERYGVTE
                     VEALLDSCHALMNYGVDRYKRPYPISAEEERLRQEEREEALQAQINELWRTLPINPKK
                     EADDSRGRFPSEPQENILYFFEKNAPLLEPWQREILRIVRKIAQYFYPQRQTQVMNEG
                     WATFWHYTLLNRLYDKGLVTDGFILEFLQSHTAVVYQPTFDKPYYRGINPYALGFAMF
                     QDIKRICQNPTEEDKYWFPDLVGKDWLETIKFAMQNFKD"
     gene            complement(4232..>5115)
                     /locus_tag="PLY05_06150"
     CDS             complement(4232..>5115)
                     /locus_tag="PLY05_06150"
                     /inference="COORDINATES: protein motif:HMM:NF003707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HNC02921.1"
                     /translation="LPNLIKRRLAGIETWEWQNAGIVSEGNPAKINIVRSLRAATSRR
                     IALGTSKKRSLRAKEQELAELLEQETSPERVAKIAQLREEISKLKIRLEKIPFIDTFD
                     LRYNHHIKVAKPSAQAVMFCIMDVSGSMSQVTKDMAKRFFLLLYLFLKRNYKKIEVVF
                     IRHHTSAKEVDEEEFFYSRETGGTIVSSALKLMNEIIQSRYPAEQWNIYGAQASDGDN
                     WSDDSPLCQKILEEQLLPALQYFSYIEITPRQHQALWLAYEDVLANHPDHFAMQQIIE
                     PSDIYPVFRELFARKPA"
BASE COUNT         1392 a         1052 c         1007 g         1464 t
ORIGIN      
        1 caacctaaat aagtaggttt agatagattt ttcatataca gtgtgtaacc ccacaagttt
       61 aataattaca agtttttcag tttttctaac tgttcttgca atttagcttg cgctgttttg
      121 gcatcgttga gcttcttttg ctcttgagca atcaccgcga caggcgcttt ggcagtaaaa
      181 ctttcgttac tgagtttgcc accaatcctc gttacttctt gctctaaacg ggcaatttct
      241 ttgttgagac gtgcagcttc ggcatcttta tcaataaagc ctttaagtgg tactaatact
      301 gccatatcac ccaccaaagc ggtggccgca ggcggtaatt cggcatcagc cgccaataac
      361 gttaagctgt ccaaacgtgc taaaaagcgt agttgagatt cataagcttg taaacgtgtg
      421 gcatcagccg cactgacgtt atgacataaa atcggtaaag gcttgcttgg cgaaatattc
      481 atttcaccac ggatattacg aatacctgca atcacccctt taatccattc cacatcagct
      541 tcggcctgtg catcaatttt gcttaaatca ggttgtggat aggcttggag cataatactg
      601 tcgcctgtct tgcccactaa acctttcaca cgctgccaaa tttcttcggt gatataaggc
      661 ataattgggt gcattaagcg caacatcgtt tctaaaacac gcaacaaggt acggcgtgtg
      721 cctgctttgg cggcagggct gctatcgcca tttaacacag gtttggttag ctctaaatac
      781 cagtcacagt attcattcca tacaaactca taaatcgctt gggcggcttg gtcaaaacgg
      841 aagctatcta aagcattaat tacgttttgt tcggcttgct gcaagcggct aataatccat
      901 ttgtcggcaa cgcttaattc aacattgtcg tggtcaaccg caacatcttg gccttcgcag
      961 ttgcttaaca caaaacgtgc ggcattccat aacttattgc aaaagttacg atagccttct
     1021 aaacgtttca tatcccagtt aatgtcacgg cctgttgacg ctaatgaggc aaaggtaaaa
     1081 cgtaaagcat ctgtgccata agcattaatg ccttcggggt attctttgcg ggtatttttt
     1141 tcgatttttg gcgcatcttg tggcttcatt aagccagtcg tgcgtttgcc aactaaagac
     1201 tctaaatcaa taccatcaat aatgtctaaa gggtctaaca catttccttt ggattttgac
     1261 attttttgac cgtcagcatc acgtactaaa ccatgaacat acacggtctt aaacggaatt
     1321 tgtggcgtgc catcttcatt cttcataaag tgcatggtca tcatcatcat ccgtgcgacc
     1381 cagaagaaga taatatcaaa accagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnncgcg aaatacacca atcttgaatg tcacgcatcc acgcaaaata catattttgg
     1561 tattgttcag gcacaaactt tacgcgacca tcttcaacgg cttcaatcgc aggtttggct
     1621 aataccgagg ctttaacata ccattggtca gtcaaccatg gttcaacaat cacgccagag
     1681 cggtcaccac ggggagcttt gagtttgtat ggttcaatct tatccaacca accgttttct
     1741 tcggcttctt taaccaattt tttacgggca acaaaacgct ctaagcctgc atattcgctg
     1801 ggtgcagctt cttgacctaa gtcagcaaaa gtgcctgcgg ccatcacctc aaaattcgcc
     1861 aaaatgttgg cgtctttatc aaagatgttg attaacggca ggtcgtgacg cttgcctaat
     1921 tggaagtcgt taaagtcgtg ggcaggggta attttgacgc aacctgtccc aaattcttta
     1981 tcaacataac tgtccgctac aatcggcact aaacggcctg taatgggcaa acgaactttt
     2041 ttgccaatca acgcttgata gcggtcatct tcagggttaa ccgcaacggc tgtatcaccc
     2101 aataaggttt cggggcgggt agtggcgacg actaaatggt tactgccatc ttccaaaaag
     2161 tatttgaaat gccacaatga gccattttct tcttcgctaa tcacttctaa gtcagaaatg
     2221 gcggtgtgta acttgggatc ccagttaacc aaacgtttac cacgataaat taagccatct
     2281 tgatacaaac gcacaaaagc ttctttaacg gcattagaca agccctcgtc catggtaaaa
     2341 cgttcgcgtg tccaatctac tgatgaacct aaacgacgaa tttgacgggt aatcgtgccg
     2401 cctgattgtt ctttccattg ccaaactttt tctaaaaatt tgtcacggcc taaatcatga
     2461 cggctgatgt tttgcgcggc taattggcgt tcaaccacca tttgtgtggc aatacctgcg
     2521 tggtctgtac ccggttgcca taaggtgttg taaccctgca tacggcgata gcgaattaag
     2581 ctatccataa tggtattgtt aaaaccatga cccatgtgca aagaacctgt gacattgggc
     2641 ggtggaatca taatacagta gggtgtgcct tgacctgatg gggcaaaata gccgttagtt
     2701 tcccattgtt gataccagcg agtttcgctg tcttttggat taaacgtagc gtcgagcgtc
     2761 ataacattac cgtgaattaa aacgaaagaa taaggatgta gttgtagcca gtatgtagcg
     2821 tagcgaaata cgggattaaa tcaggactta tgcaagtcct atacttagct aaaaattagc
     2881 ttaatagtaa cagattttga gggggggcaa caaaacatca atagccatag attttgttta
     2941 gacttgagac ataaaggcat tttaaatgcc tctatgtttt ttagccttgt ttaaaggttt
     3001 taatgccttc tttgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgtct
     3121 ttaaagtttt gcatggcaaa tttaatggtt tctagccaat ctttaccaac caaatcggga
     3181 aaccaatatt tgtcttcctc agttggattt tgacaaatac gtttaatgtc ttggaacatg
     3241 gcaaaaccca aagcataagg attgatgcca cgataataag gcttatcaaa cgtgggttga
     3301 taaacaacag ccgtatgtga ttgtaaaaac tctaaaataa agccatcagt caccaaacct
     3361 ttatcgtata aacgatttaa taaggtgtag tgccaaaagg ttgcccaacc ttcattcatg
     3421 acttgggttt gtcgttgagg ataaaaatat tgggcaattt tacgaacaat tcttaaaatt
     3481 tcacgttgcc acggttcaag caagggcgca tttttttcaa aaaaatataa aatgttttct
     3541 tggggttcag acggaaaacg cccacgactg tcatcggctt cttttttggg gttaatcggc
     3601 aaggtgcgcc acaattcatt aatttgtgct tgtaaagctt cttcgcgttc ttcttggcgc
     3661 aaacgctctt cttcggcaga aatagggtag gggcgtttgt aacgatcaac cccataattc
     3721 attaacgcat gacaagaatc taatagcgct tcaacttcag tgaccccata acgttcttca
     3781 cacttgcgaa tatagtttcg ggcaaagact aaataatcaa taatgctgct cgcatctgtc
     3841 catgttttaa ataaatagtt gcctttaaaa aaagaattat gaccataaca agcatgagca
     3901 atcactaagg cttgcatggt catggtattt tcttccatta aataggcaat acagggattg
     3961 gagttgatga caatttcata ggccaacccc atttggcctc gttgataagc attttcggtg
     4021 ctgacaaaat gtttgccaaa agaccaatga tgataaccaa tgggcatccc taccgaggca
     4081 tacgcatcca tcatttgttc ggctgaaatc acttctattt gattagggta ggtatctata
     4141 ccaaattcac catgagcaat ttcggcaatg gcggcatcat attcggcaat aagagggaaa
     4201 gaccattctg atcctgttga taaataacga ctcatgcggg cttcctcgca aatagttcac
     4261 gaaatacggg ataaatatca ctgggctcaa taatttgctg catggcaaaa tgatcgggat
     4321 ggttagctaa aacgtcttcg taagctagcc ataaagcctg atgttgtcgt ggcgtaattt
     4381 cgatataaga aaaatattgc aaggcgggta ggagttgctc ctctaaaatt ttttggcaca
     4441 acggtgagtc atcgctccag ttatcaccat ctgatgcttg agcaccgtaa atgttccatt
     4501 gttcagcagg ataacggctt tgaatgattt cattcatcag tttgagtgcg ctggagacaa
     4561 tcgtaccacc tgtttcacga gagtaaaaaa attcttcttc gtcaacttct ttggcactgg
     4621 tgtggtgacg aataaaaacc acttcgattt ttttgtagtt acgctttaaa aataaataca
     4681 ataataaaaa gaagcgtttg gccatgtctt tagtcacttg gctcattgag ccagatacat
     4741 ccataataca aaacatcact gcttgggcag agggtttggc gactttaata tgatggttat
     4801 aacgtaggtc aaaggtatca ataaacggaa ttttttctaa acgaattttt agtttagaaa
     4861 tttcttcacg caattgcgcg attttggcca ctcgttcggg cgaagtttct tgctctaaaa
     4921 gttcggccag ttcttgttct ttggcacgta agctacgttt tttacttgtc cctaaggcaa
     4981 tacgccgaga agtggcagca cgtaagctgc gaacaatatt aattttggca ggatttcctt
     5041 cgctaacaat acctgcattt tgccattccc atgtttcgat gcctgcaagt cgtcttttaa
     5101 tcaggttggg taggg
//
LOCUS       DAOQMB010000142         5090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7548_length_5090_cov_17.859384, whole genome shotgun sequence.
ACCESSION   DAOQMB010000142 DAOQMB010000000
VERSION     DAOQMB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7548_length_5090_cov_17.859384"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="lepA"
                     /locus_tag="PLY05_06155"
     CDS             <1..1173
                     /gene="lepA"
                     /locus_tag="PLY05_06155"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNC02922.1"
                     /translation="VSLVRVRHGRIKKGDRMLVKSTGQSHYVTSVGVFNPKHTETGSL
                     EAGEVGFVIAGIKDIFGAPVGDTITHHATPDVEILPGFKTVKPQVYAGVFPVDSSDFE
                     AFREALQKLKLNDSALFFEPESSDALGFGFRCGFLGMLHMEIVQERLEREYNLDLITS
                     APTVIYEAKKKNGDIVYVDNPSKLPEGSIIEELREPIAEVHILVPQEYVGNVMTLSTE
                     RRGVQKDMKFLGNQVALSYDIPMAEVVLDFFDKLKSTSRGFASLEYGFNRFETAKLVK
                     VDVLINGEKVDALAMICHANDARHRGLSLVDKMKDLIPRQMFDVAIQAAIGSQIIARS
                     TVKAMRKNVLAKCYGGDVSRKKKLLEKQKEGKRRMKQVGSVEIPQEAFLAVLKVDR"
     gene            1173..1955
                     /gene="lepB"
                     /locus_tag="PLY05_06160"
     CDS             1173..1955
                     /gene="lepB"
                     /locus_tag="PLY05_06160"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNC02923.1"
                     /translation="MDFDFALILTVITLVTGLICLADKLFFKQHQAVLNTAKSKQPAK
                     EHIIIEYAYSFFPVLAVVWVLRSFLFEPFTIPSGSMLPTLEVGDYILVNKYQYGLRLP
                     VLGTEIMPIGKPKRGDVMVFKYPPKPSMNFIKRVVGLPGDKVNFVNDTLYINGEAIER
                     KLLSQEPEVEPWQQYFEENLSGTKHTIRQEVGRYPAGKQWGYTVPEGHYFMMGDNRDN
                     SNDSRFWGFVPEKLIVGKAVYIWTHKEPGFNLPSFHRNSSIQ"
     gene            1981..2367
                     /locus_tag="PLY05_06165"
     CDS             1981..2367
                     /locus_tag="PLY05_06165"
                     /inference="COORDINATES: protein motif:HMM:NF027463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HNC02924.1"
                     /translation="MRKYQQGLSYWSVMFAVCFFAFLLKAGATIGPVYLDYYTLDKML
                     QAKFRETQVDKLEVKQFVKDLSAQMERNGIRDRTVEDLMVIKREGNLLIVELDYEERK
                     NFASNLDIVAHFKKNYSTEKPDGYVE"
     gene            2367..3041
                     /gene="rnc"
                     /locus_tag="PLY05_06170"
     CDS             2367..3041
                     /gene="rnc"
                     /locus_tag="PLY05_06170"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNC02925.1"
                     /translation="MIYQRLMSALGYKFKDEAWLKLALTHRSVSGNHNNERLEFLGDS
                     ILGMIVAEYLFQQFPKEKEGRLTRLRANLVKQDSLAEIARQLKLGDYLHLGAGELKSG
                     GFRRDSILADALEAILGAIYLDAQSIDPCRECLLRWYGQRLNDVAIEVTLKDPKSRLQ
                     EYLQSRHLALPTYSVQATWGEDHNQTFQVQCIIDNIPPVLATGASRRHAEQAAATLIL
                     QHLGVQ"
     gene            3046..3993
                     /gene="era"
                     /locus_tag="PLY05_06175"
     CDS             3046..3993
                     /gene="era"
                     /locus_tag="PLY05_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004939245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HNC02926.1"
                     /translation="MTDQVIENFFGNTQQEIDSENYRCGFVAIVGRPNVGKSTLLNHL
                     LGQKLSITSRKPQTTRHKIVGIVTENNVQAIYVDTPGMHKREPRAINRLMNQAAQSAL
                     KDVDLILFVVDGLKWTPDDELVLEKLAKVDTPVILVVNKVDTLTDKAVLLPYLETLNQ
                     KRHFSEVIPLSALRGHNLSTLHDIVGRYLPYAPPMYAEDQITDRSQRFLAAEMIREKI
                     MRQMGDEVPYDLMVQIDQFKIEGRLYRIDATILVERDGQKAIVIGEGGQKLKTIGQAA
                     RLDMEKLFDSKVMLTLWVKVKGGWSDDERALKSLGYGDV"
     gene            3993..4661
                     /gene="recO"
                     /locus_tag="PLY05_06180"
     CDS             3993..4661
                     /gene="recO"
                     /locus_tag="PLY05_06180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNC02927.1"
                     /translation="MTEIAPLCHGYLLHSRPYREKSRLTEWWTLEHGRISAVMRQNPP
                     PLFQPCLMAWRGKRPLKTLQNHESVGLPYILEGNATFAGFYLNELLVRLLAYEEAHTE
                     LFVLYSQTLASLKDNPHDLEPTLRHFERCLLASLGYALDFRIDAQQQVIQPNNFYLYE
                     PQNGFVLIDKIEHNVWQGKTLLAIAAEDWQLNTTKQAAKKIIRAALAVHLGNKPLKSR
                     ELFI"
     gene            complement(4698..>5090)
                     /locus_tag="PLY05_06185"
     CDS             complement(4698..>5090)
                     /locus_tag="PLY05_06185"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HNC02928.1"
                     /translation="WRGRAEIQVSSKDFKSAGELISQLQSKLQLNNLNFSVAPETRRE
                     LENQLISEAVAAFKTRADKIKTAWSAKGYKLIQMNLGTTNNQPPQPMYMMARAAKMEM
                     ADGAPAADYAGGQSRLNVQVSGSIELEQ"
BASE COUNT         1526 a          880 c         1116 g         1568 t
ORIGIN      
        1 gtttctttag ttcgtgtacg tcatggccga attaaaaaag gcgaccgtat gttggtaaaa
       61 agtactggcc aatcacatta tgtgactagt gttggtgttt ttaatcctaa acataccgaa
      121 acaggtagcc ttgaagcagg tgaagtaggc tttgtgattg caggtattaa ggatattttt
      181 ggtgcgcctg ttggtgatac cattactcat catgctacgc cagatgtaga aattttacca
      241 ggttttaaaa ccgttaaacc gcaagtttat gctggcgtgt ttcctgtaga ctccagcgac
      301 tttgaagcat tccgtgaagc cttacaaaag ctaaaactta atgattcggc cttatttttt
      361 gagccagaat cctctgatgc gctaggtttt ggttttcgtt gtggtttctt gggcatgttg
      421 cacatggaaa tcgttcaaga acgtttagag cgtgaataca atttagactt aattacctct
      481 gcacctaccg ttatttacga agccaaaaag aaaaacgggg acatcgtgta tgtggataac
      541 ccttcaaaat tacctgaagg ttcaattatc gaagagttac gtgaaccaat cgctgaagtg
      601 catattttag tgccacaaga atatgtgggt aatgtcatga ccctatcgac cgagcgtcgt
      661 ggtgtgcaaa aagatatgaa gttcttgggg aatcaagtag cgttgagcta cgacattccc
      721 atggccgaag tggtgttaga tttctttgat aaacttaaat caacctctcg tggttttgcc
      781 tctttagagt atggttttaa tcgttttgaa accgctaaat tggtgaaagt ggacgtgttg
      841 attaacggtg agaaagttga tgccttggca atgatttgtc atgccaatga tgctcgtcat
      901 cgtggtttat cgttagttga taaaatgaaa gatttaattc cacgtcaaat gtttgatgtt
      961 gccattcaag cggcaattgg tagccaaatt attgctcgct ccacggtaaa agcaatgcgt
     1021 aaaaacgtat tggctaaatg ttatggtggt gacgtatcgc gtaaaaagaa acttctcgaa
     1081 aaacaaaaag agggtaaacg ccgcatgaaa caagtgggta gcgtagagat tccacaagaa
     1141 gccttcttag ctgtattaaa ggtagataga taatggattt tgattttgca ctcatattga
     1201 cagttataac cttggtaacg ggtctaattt gtcttgcaga taaattgttt tttaaacagc
     1261 atcaagctgt cttgaatacg gcaaaatcaa aacaacctgc caaagagcat attattattg
     1321 agtatgctta ttcatttttt cctgttttag ctgtcgtttg ggtgttacgc tcatttttgt
     1381 ttgagccatt taccattcca tcaggttcaa tgttgcccac tttagaggtt ggcgactata
     1441 ttttagtgaa taaataccaa tatggtttac gtttacctgt gttaggcaca gaaattatgc
     1501 ctattggtaa gccaaaacgt ggtgatgtta tggtatttaa gtacccacct aagcccagta
     1561 tgaattttat taagcgtgta gtcggcttac caggcgataa agtgaacttt gtcaacgaca
     1621 cactgtatat caatggtgaa gcgattgagc gtaaactact ctctcaagag ccagaggtag
     1681 agccgtggca gcaatatttt gaagaaaatc tgagtggtac taaacacact atccgtcaag
     1741 aggtaggtcg atatccagcg ggtaaacaat ggggctatac tgtaccagaa ggtcattatt
     1801 ttatgatggg cgacaatcgt gataatagta atgacagtcg tttttgggga tttgtgccag
     1861 aaaaattaat tgttggtaaa gcggtttata tttggacaca taaagagcca ggttttaatc
     1921 tgccaagctt tcatcggaac agtagtattc aataataact atcattaaga gggtaagggt
     1981 atgcgtaagt atcaacaggg gttgtcatat tggtcggtga tgttcgctgt ttgttttttt
     2041 gcttttttat taaaagcagg agcgacaatt ggacctgtct atttggatta ttatacttta
     2101 gataagatgc tacaagcaaa gtttagagaa acacaagtcg ataaattaga agtaaagcag
     2161 tttgttaaag atctaagtgc acaaatggaa cgtaatggca ttcgtgatcg tactgtagaa
     2221 gatttaatgg taattaaacg cgaaggtaat ttattaattg ttgaattaga ttatgaagag
     2281 cgtaaaaatt ttgcatccaa tttagatata gtcgctcatt tcaaaaagaa ttattctaca
     2341 gaaaagccag atggatatgt agaataatga tatatcaacg tttaatgagt gctttaggat
     2401 ataaatttaa ggatgaagca tggctcaagt tagcactgac gcatcgtagt gtcagtggca
     2461 atcacaataa cgaacgatta gagtttttag gtgactcaat tcttggaatg atagttgctg
     2521 aatacttatt tcagcagttt cccaaagaaa aagaaggacg tctaactcgg ttacgtgcta
     2581 atttggttaa gcaagacagt ttggcagaga tcgcccgaca attaaaattg ggcgattatt
     2641 tgcatttagg tgcaggtgag ttaaaaagcg gtggttttcg ccgtgactct attttagcgg
     2701 atgcgttaga ggcaatttta ggggcaattt acttagatgc ccaaagcata gatccctgtc
     2761 gtgaatgctt attacgttgg tatggccaac gtttaaatga tgttgccatt gaggtgactt
     2821 taaaagaccc caaaagccgt ctgcaagaat atttgcaatc acgccattta gcgttgccaa
     2881 cctatagtgt gcaagcaaca tggggtgaag atcacaatca aacctttcaa gttcaatgta
     2941 tcattgacaa tattccacct gttttagcaa caggtgctag tcgccgtcat gctgagcaag
     3001 cagcggccac acttattctt caacatttag gtgtacagta atcatatgac tgaccaagta
     3061 atcgaaaact tttttggtaa tacccaacaa gagattgact cagaaaacta ccgctgtggt
     3121 tttgttgcta ttgtcgggcg gccaaatgtc ggtaagtcta ccttattaaa ccatttatta
     3181 ggtcaaaagt tatctattac ctcacgtaag ccacaaacca cacgccacaa aatcgttggt
     3241 attgtcactg aaaataatgt gcaagcgatt tatgttgata caccgggtat gcacaaacgt
     3301 gagccaagag cgattaatcg tttaatgaat caagcagcac aaagtgcctt aaaagacgta
     3361 gatttgattt tatttgtggt tgatggttta aaatggacac ctgatgatga gttggtgtta
     3421 gaaaaattag caaaggtaga tacgcctgtt atcctcgttg ttaataaggt tgatacttta
     3481 acggataaag cggtgttatt accttattta gaaactttaa atcaaaaacg tcatttttca
     3541 gaagtgattc ctttgtctgc attacgtggt cataatttgt cgacattgca tgatattgtt
     3601 ggccgttatt taccctatgc accacccatg tatgccgaag accaaattac cgatcgttca
     3661 cagcgctttt tagccgcaga aatgattcgc gaaaaaatta tgcgtcaaat gggtgacgaa
     3721 gttccttatg atttaatggt acaaattgat caatttaaaa tagaaggtcg tttgtatcgt
     3781 attgatgcaa ctattcttgt tgaacgtgat ggccaaaagg ccattgtgat tggtgagggc
     3841 ggtcaaaagc taaaaaccat tggccaagca gctcgtttgg acatggaaaa gctttttgac
     3901 agtaaagtga tgctcacgtt atgggtgaag gtcaagggtg gttggtcaga cgatgaacga
     3961 gccttaaaaa gcttgggcta tggtgatgtt taatgactga aattgcgccg ttatgtcatg
     4021 gatacttatt acatagtcgg ccatatcgag aaaaaagtcg tttaacagag tggtggacct
     4081 tagagcatgg ccgaattagt gcagtcatgc gccaaaatcc gccgcctttg tttcagccct
     4141 gtttaatggc atggcggggt aaaaggccac taaaaacttt gcaaaatcat gaaagtgtcg
     4201 gcttgcctta tattttagaa ggtaatgcta catttgccgg tttttatctt aatgagctat
     4261 tagtgcgctt attagcttat gaagaagcgc ataccgaact gtttgtgtta tatagtcaaa
     4321 ctttagcgtc tttaaaagat aacccccatg atctagaacc tacattacgt cattttgaac
     4381 gctgtttatt agcaagcttg ggttatgctt tagattttcg tatagatgct cagcaacaag
     4441 tgattcaacc aaataacttt tatttatatg agccgcaaaa tggttttgtg ctaattgata
     4501 aaattgaaca taacgtttgg caaggcaaaa cgttattggc aattgcggct gaagattggc
     4561 aattaaatac caccaaacaa gcagctaaaa aaattattcg tgcagcttta gcagtacacc
     4621 taggtaataa accgctaaaa agtcgagagt tatttatctg aaagtccctc ttcttgtgga
     4681 agagggctag ggtgaggtca ttgctctagt tcaatactgc cagacacctg tacatttaag
     4741 cgactttgac cgcccgcata gtcagcagca ggtgcaccat cagccatttc catttttgca
     4801 gcacgagcca tcatatacat tggctgtggc ggctgattgt tggttgtgcc taaattcatt
     4861 tgaatgagtt tataaccttt agcactccat gctgttttaa ttttatcagc acgagtttta
     4921 aaggctgcaa cagcttcact gattaattga ttttctaatt cacggcgagt ttcaggggca
     4981 acactaaagt ttaagttgtt taactgtagt ttactttgta attggctaat taactcacca
     5041 gcagatttaa aatctttact agagacttga atctccgcac ggccacgcca
//
LOCUS       DAOQMB010000143         5055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7646_length_5055_cov_42.443800, whole genome shotgun sequence.
ACCESSION   DAOQMB010000143 DAOQMB010000000
VERSION     DAOQMB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5055
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7646_length_5055_cov_42.443800"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            873..>1621
                     /locus_tag="PLY05_06190"
     CDS             873..>1621
                     /locus_tag="PLY05_06190"
                     /inference="COORDINATES: protein motif:HMM:NF020290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PriCT-2 domain-containing protein"
                     /protein_id="HNC02929.1"
                     /translation="MWSFEQYEGDKKPRKVPYYINGKKRQGTQGSPEDLKNLATYEQA
                     KSALEFGDYSGLGFAFLPNDGLIGIDIDHNEQRDPQLAQKIITGLNTYTELSPSGKGY
                     HLFVLGNTKTFKSNDIGIEVFANAQFFTMTGNQVDGTPNEVNQISEKALNRLREIVKP
                     PRENKPKNESQLPINERAKVESALAYIPADCGYDDWWKIGAAIYSEFGASGFSIWDYW
                     SAKSSKYSPRGMNNKYNSFANIHDIKIATVYR"
     assembly_gap    1622..1721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1722..2819
                     /locus_tag="PLY05_06195"
     CDS             <1722..2819
                     /locus_tag="PLY05_06195"
                     /inference="COORDINATES: protein motif:HMM:NF017116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE family protein"
                     /protein_id="HNC02930.1"
                     /translation="LLIPNAKFSIDNQANAALAHVLSWVNRFGMSTGNVQDFVTAIAD
                     NNQYNPVANWILSKEWDKKPRLMDFWSTIKEKEPHTLADGRQLKDVLLMRWMVSCVAA
                     VFNPDGISAHGVLVLQGDQYLGKTKWMKSLAPKELGVIKDGITLNPSDKDSVMQCVRN
                     WIVELGELDATFRKSDIASLKSFITSDRDVLRRPYARLESEFARRTVFFASVNPKQFL
                     HDPTGNRRFWTIECEYIDHSHTFDMQQVWAETYEQLYKKGVKCYLEPDEMAALNSRNE
                     SHTSDEPIKDLIDTAFNWLAPKAAWNYKSTATDICIWAGMQKPTKADINAASQHVTKK
                     YNVKTTYSNGRKVWLMPPRLNVIAQDPAFGE"
     gene            3023..4471
                     /locus_tag="PLY05_06200"
     CDS             3023..4471
                     /locus_tag="PLY05_06200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02931.1"
                     /translation="MINLRDYQQKAVAAIRHAYANKKRAPLLVLPTGGGKTTIFAYVT
                     QQASLKNNCVFLLCHRAELVKQISMTLAKFDVCHQVIAPPVIATQCRNAHYLEFGKAY
                     IAPSKVFVCSVQTLIKRLSTMPTPELIVCDEAHHLTPDSTWGKIIQSYPNARVLPVTA
                     TPCRLDGKGLKNICDDMIIGETMENLIKQGYLCNYRAFCPPNALDLTTVKKTAGDYNK
                     GQLAEAMDKPTITGDAVKHYLKLCNGKRAVAFCVSVAHAEHVAESFNLVGVPSESLDG
                     TLSPEERDARIKRFERGETLVLTSCDVISEGFDLPAIEAAILLRPTQSLSLYLQQVGR
                     ALRTFSGKKEAIILDHVGALSRHGLPDMDREWTLDGVSKKERQKNDEPDINITTCKQC
                     FGVFLAKQNQCPFCQWVKPAPKGREIKQEEGELTEFDKEQFKLKLKLETRAARDYDSL
                     VALGRQRGYKSPEFWARKQLEIREAYRAKSRN"
     gene            4476..4775
                     /locus_tag="PLY05_06205"
     CDS             4476..4775
                     /locus_tag="PLY05_06205"
                     /inference="COORDINATES: protein motif:HMM:NF020354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HNC02932.1"
                     /translation="MNKYLLHFSGAGAMVWRNNTGKFRSLTDPQRIVSVGQVGSADII
                     GVQPVTITPEMVGKVIGQAIAIEVKTPKGRQSEEQKNWQKAFEQHGGIYILGRGI"
BASE COUNT         1649 a          962 c         1016 g         1328 t
ORIGIN      
        1 gattgtcatt ttttagctcc acataaactt gtctagcttc agactttagc tgctcaagtt
       61 tatctgctcc gtgttttttg tcgtacaact caaaaaaacg gtcacgttcg ggcttttcta
      121 atcggcaaac gtatcttaat aagcagtggt gtttatattg ttcggtgttc ataggttgac
      181 aagtgtcctt ctagtgttta acaattttag ttgtccacca caaaaaaatc aatatatatc
      241 aatgctttaa tacttaaaag tgtacaaggt gtatatatat tatgtatata ataggatgta
      301 acaataggta tatagtatat ttatacttaa tatagggaaa cgagaaaaag acgtacactt
      361 aaacactaag aatcgcgcta agccttgcgg ctctaaggct ggcgcgtggt gtccaactaa
      421 tccacttgac caccaaaaac taacatgata cattaaacag attaaataca ttaaaaggct
      481 atttattatg tatctcattg aaaactcaaa caaaacaatt ttagagacga actggaggca
      541 tcaaaaatac ccatttcaac aaatggagat aggggagtat tttttagtgc caaaaactga
      601 ggtcaaacaa agcagtttgg ccgcgtccgc gtcttgggct agtaaaaaat tcggaaaaaa
      661 gtttataaca gagacttttg gcgaatttac aaaagttact agagtaaaaa aaccaaagac
      721 tgtatgatta ttttgcggct agactagacg gccatctagt gacaaaagcg attcgttacc
      781 gcttgccgcg cactatgtaa cgcataaaac caacaacgta aggtatcaat aatatgttcc
      841 cagtcgaatt aacgagccgt aaacagtggc ttttgtggtc atttgaacaa tacgaaggcg
      901 acaaaaaacc gcgcaaagtt ccgtattaca tcaacggtaa aaaacgtcaa ggcacacaag
      961 gcagccccga agatttaaaa aacttagcga cttacgaaca agcaaaatct gcattagagt
     1021 ttggcgatta tagcggctta ggttttgctt ttttgcctaa cgatggattg attggcattg
     1081 atattgacca caacgaacag cgcgacccac agttagcaca aaaaatcata acaggattaa
     1141 acacatacac cgaattatcc ccatcgggta aaggctatca tttatttgtt ttgggcaata
     1201 caaagacatt caaaagcaat gatataggca ttgaagtttt tgcaaatgcc caatttttta
     1261 caatgacagg aaatcaggtt gacggcacac ccaacgaagt taatcaaatt tctgaaaaag
     1321 ccttaaatcg cttacgtgaa attgttaaac caccacgcga aaataaacct aaaaatgaat
     1381 cacaactgcc gattaatgaa cgcgcaaaag ttgaatctgc attggcttac atccctgccg
     1441 attgtggtta tgatgattgg tggaaaatag gtgcggcaat ttatagcgag tttggcgcat
     1501 caggttttag catttgggat tattggtcag ctaaaagcag caaatacagc ccacgcggca
     1561 tgaataataa atataattcg tttgctaaca tccacgacat caaaatagcc acagtttacc
     1621 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nctattgata cctaatgcca
     1741 aattcagcat cgacaaccaa gccaatgctg cgcttgcaca cgttttatcg tgggtcaatc
     1801 ggtttggaat gtccacaggc aatgttcaag attttgttac agctattgca gacaataacc
     1861 aatataaccc tgttgctaat tggattttat cgaaagaatg ggataaaaaa ccgcgtttaa
     1921 tggatttttg gtcaacaatt aaagaaaaag aaccacacac gctagccgat ggccgccaat
     1981 taaaagatgt tttattaatg cgttggatgg tgtcatgcgt cgccgccgtt tttaatcctg
     2041 acggcatatc agcgcatggt gttttagttt tacagggtga ccaatatcta ggtaaaacaa
     2101 aatggatgaa gtcattagca cctaaagaat taggcgtaat caaagacggt attacattaa
     2161 acccaagcga caaagacagc gtaatgcaat gtgttcgtaa ctggattgtt gaattaggcg
     2221 aactagacgc aacgtttcgc aaatctgata ttgcgagttt aaaatcattt atcacatccg
     2281 accgcgatgt attgcgtcgc ccttatgctc gattagagag tgaatttgct agacgcaccg
     2341 tgttttttgc atcggttaac cctaaacagt ttttacacga ccctacaggc aatagacgtt
     2401 tttggacaat tgaatgtgag tatatagatc actcgcacac gtttgatatg cagcaagttt
     2461 gggctgagac ctacgaacag ttatacaaaa aaggtgttaa atgttattta gagcctgacg
     2521 aaatggccgc gcttaatagc cgtaacgaat cgcatacatc agacgaacct ataaaagatt
     2581 taattgatac cgcgtttaat tggcttgcac caaaagccgc ttggaactat aaaagcaccg
     2641 ccaccgatat ttgcatttgg gcaggtatgc aaaaaccaac aaaagcagat attaacgcgg
     2701 catcacagca cgtcacaaaa aaatacaatg taaaaacaac atacagcaat gggcgcaagg
     2761 tttggctcat gccaccgcgt ttgaatgtta tcgctcaaga cccagcgttt ggggagtaat
     2821 atggaatgta aacatattgg ccacagagta tttacaaaac gattatttca aaatctaaca
     2881 acacactact gtgtacaatg tttgaattgt gggcagatag ttaagtataa caacaaaata
     2941 tggttaaaac tggaggatat accaccaaca aaaacaattc ataattttga tgaatttttg
     3001 cttgaaaatg gaggtttaaa aaatgattaa cctccgagat tatcagcaaa aagctgttgc
     3061 tgccattcgc cacgcttacg cgaacaaaaa acgcgccccg ttgttggtat tgcccacagg
     3121 tggcggtaaa accacgatat ttgcttacgt tacacaacaa gcaagcctaa aaaacaattg
     3181 tgtgttttta ctatgccacc gtgccgagtt agtcaaacag ataagtatga cattagccaa
     3241 gtttgatgtt tgtcaccagg taatcgcgcc gcctgtcatc gccacccaat gccgcaacgc
     3301 gcattattta gaatttggta aggcatacat tgcaccgtca aaagtgtttg tttgcagtgt
     3361 acaaacattg ataaaacgat taagcaccat gccaacacct gaattgattg tgtgcgatga
     3421 agcgcaccat ctcacacctg atagcacatg gggcaaaatt atacagtcgt accccaatgc
     3481 gcgagtttta ccagtcaccg ccaccccatg ccgattagac ggcaaaggat taaaaaatat
     3541 atgtgatgat atgataattg gcgaaacaat ggagaaccta attaaacaag gttatttatg
     3601 caattatcgc gctttttgcc cacctaatgc actggattta acaaccgtaa aaaaaacggc
     3661 aggggattac aacaaaggac aattagcgga ggccatggac aaaccgacaa taacaggcga
     3721 cgcggtaaaa cactacctaa aactatgcaa cggcaaacgc gctgttgcgt tttgtgtatc
     3781 ggttgctcat gccgaacacg ttgcagaatc gtttaattta gttggtgtac caagcgaatc
     3841 gttagacggc acactatcac ccgaagaaag ggatgcacga ataaaacgct ttgaacgtgg
     3901 agaaacactt gtattaacat cgtgcgatgt tattagcgaa ggctttgatt tgccagcgat
     3961 tgaagccgcg atattgttac gaccaacaca aagtcttagt ctgtatttgc agcaagtagg
     4021 ccgcgcattg cgtacgttta gcggtaaaaa agaagccatt attttagacc atgttggcgc
     4081 gttatcacgt catggtttgc ctgatatgga tagagaatgg acgttagacg gtgttagcaa
     4141 aaaagagcgt caaaaaaatg acgaacctga tataaatatc acaacttgca aacaatgttt
     4201 tggtgttttt ttagcaaaac aaaatcaatg tccattttgc cagtgggtaa aacctgctcc
     4261 aaaaggcagg gaaataaaac aagaagaagg tgaattgaca gagtttgaca aagagcaatt
     4321 taagttaaaa ctcaagctag aaacgcgggc agctcgtgac tatgattcat tagtcgcgct
     4381 tggtcgtcaa cgcggctata aatcgcctga gttttgggcg cgtaaacaat tagaaattag
     4441 agaggcgtat cgtgcgaaat caagaaacta acattatgaa taaatacttg cttcattttt
     4501 caggagcagg tgcgatggtg tggcgaaaca acactggcaa atttagaagc ctgacagacc
     4561 cacaacggat tgtgtctgtc ggtcaggttg gcagtgctga tataatcggg gtacaacctg
     4621 tcacaataac gcctgagatg gttggaaaag ttatcgggca agccatagca atagaagtaa
     4681 aaacacccaa aggccgccag tcagaagaac aaaaaaactg gcaaaaagcc ttcgagcaac
     4741 atggagggat ttatatttta ggtcgtggta tttgattttt tcttatttgg ctttaattcg
     4801 tttaactcga ccaagctagg gtttggatat ttaaactctt ttaagagttg ataaaacggc
     4861 aatctgtaag tacggctgat atagtttgac gtcacccaat tagatactgt ttgattagtc
     4921 actcctaatc gctttgctac atatttttgg ctgccaaagt ggtcaaccat ctctttaact
     4981 gttcgcattt ttgcgccctc ttgttttgtg tcagtgcaat gtaatataaa cattttataa
     5041 aatcaatata aaata
//
LOCUS       DAOQMB010000144         5018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7730_length_5018_cov_8.879105, whole genome shotgun sequence.
ACCESSION   DAOQMB010000144 DAOQMB010000000
VERSION     DAOQMB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_5018_cov_8.879105"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1178
                     /locus_tag="PLY05_06210"
     CDS             126..1178
                     /locus_tag="PLY05_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004678766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02933.1"
                     /translation="MLKQASIKNLTVFSQANLEFASGLNIIVGENGMGKSHLLKVLYA
                     IIAAGCDPKSTTIPQPTQSHLQKAYADKLVKVLRPDGLGRLARRKQGRERCEIGLVFA
                     EPSANIQLSLSTASKSEVQIENAPTQWQAKSPVFFPTRELLTLCPWFIGFYDNYHLKF
                     EETWRDTCSLLIAPKLKGAREKQVANMLKPLEQAMGGKVVVDDLGNFYLQVTGEGKLE
                     MPLVAEGLRKLAMLAQLISTGTLLEQGYLFWDEPETNLNPKLIKVLAGVILELAKNGI
                     QVFIATHSLFLLRELEILAASKSFEKVDKRFFGLQPTEEGVEVAQGKTIEDIGDITAL
                     EESLQQSDRYLAISWH"
     gene            1198..1692
                     /locus_tag="PLY05_06215"
     CDS             1198..1692
                     /locus_tag="PLY05_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02934.1"
                     /translation="MTINVDGLKFDFETNWQASKYDEWSYYRNQFVKQFDGIKAVDLL
                     AISPDKITFFIEVKDYTHPDTEKPSNLPQAVADKVFDTLAALLPCSLLANDANEKAFA
                     KRVIQSKQLCVVLHMEQPRAHRPVVDPADILQKLKKLLRAIDAHPKVVSIQNNPLLPW
                     TVSK"
     gene            1692..4514
                     /locus_tag="PLY05_06220"
     CDS             1692..4514
                     /locus_tag="PLY05_06220"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HNC02935.1"
                     /translation="MSLKPWREIARPHKDVLEGTFKQSEFAADITQVANGTATPEYQD
                     AEKFFNRTFITEGMRLLLISVAQRLSGLGGDPVIQLQTAFGGGKTHTMLAVFHLATRK
                     VSTEKLLGVGPLLDKAGIFELPQARVAVIDGIKLSPSQPRKYGTVQINTLWGELAWQL
                     LGNEGYTLVAASDIDGTSPGKEILTELIRKAAPCVILIDELLAYVRQLELGQHYKGGT
                     FDSNITFIQALTEAMKAVPNSILLASLPQSELEVGGTMGQRALNSLEKYFARVETVWK
                     PVATEEAFEIVRRRLFEYPGDRAEVEGISRQFSDFYRKHAEKFPVETQSNEYFERLCH
                     SYPIHPEIFDRLYEDWSTLDKFQRTRGVLQYMAIVIHRLWNSENRDALIMPGSIPLED
                     GNVRDKSIHYLSPGWEPVIEREVDSIRSIPHDIESHSTLFGGVQAARRSARTIFLGSA
                     PSNSSQIVRGINVEHILLGAVQPGQSVGIFEDVLKRLRDRLHYLYSDQGRFWLDTKPN
                     LRREMESRKATINERDELIPLLKNQVTEIFGKNHHFSGIHVFTPSVDIPDDYGIGTRL
                     VVLPLNAGYSRSEVNSAFTAATEILRNRGEQPRQKQNRLIFMAADFDVVSRLKEQGRI
                     YLAWKSIVFDIDNGTLNQDLAHLNQAKRNRDGAIQTLKQLVREAYKWLLAPIESFVNG
                     KPNLTWEAVALSTTAPNFIQEVESRLREEEWVIFEWSPIHLRNMLKQWYLKNDVQEVN
                     ALKVWQDTCHYLYLPRLVNDQVFKNAIAQGVSSQDYFAFASAKEESGRYLGFSFGHNV
                     ISTLDGSSLLIDREAALAYLERIRPVPQPALDETSSEPIVIKPTGSTPSVAPVSDPRP
                     VATSAKTKKQFYGSISLDPVKAKMEFATIIDEVIQQFTSRLGVDVTISVEVQANSSMG
                     FDDNLQRAIKENCKVLKFNTAEFEEE"
BASE COUNT         1525 a          962 c         1075 g         1456 t
ORIGIN      
        1 gggcagaaga cgcacgaact tataacgagc tgattatttc gtggtcaacc attgtcgctg
       61 cttctcatga aagtgaaact ggctatgctg gcacacaacc cagtttagat ttgtaagagg
      121 aaattatgct taaacaagcc tctattaaaa atttaacggt tttttctcag gctaatcttg
      181 agtttgcctc tggcttaaat atcattgttg gcgaaaatgg catgggtaaa tcgcacttgc
      241 ttaaagtgct atatgccatt attgccgcag gttgcgaccc caaaagcacc accattcctc
      301 agcccacaca aagccatttg caaaaagcct atgccgataa actggttaaa gtgttacgtc
      361 ctgatggctt aggccgatta gctcgacgca agcaaggccg cgaacgttgc gaaattggct
      421 tggtgtttgc cgaaccatct gcaaacattc agttaagctt gtcaactgcc agtaaatccg
      481 aagtacaaat agaaaacgca ccaacacaat ggcaagctaa atcacctgtc tttttcccta
      541 cccgagaact actgaccctt tgtccgtggt tcattggttt ttatgataac tatcacctta
      601 aatttgaaga aacatggcga gatacctgtt cgttactcat agcacccaag ttaaaaggag
      661 cgcgtgaaaa gcaggttgca aacatgctta aaccgcttga gcaagcgatg ggtggcaaag
      721 tagtggttga tgatttaggt aatttttatt tgcaagtcac tggtgaaggc aagctagaaa
      781 tgcccttagt ggcagagggt ttgcgtaagc tggccatgtt ggcacaactg attagtactg
      841 gcacgctact cgaacaaggt tatttatttt gggacgaacc cgaaacgaat cttaatccta
      901 agctcattaa agtattagca ggcgtgatat tagaattggc taaaaatggt attcaggtgt
      961 ttattgcaac acatagcctg tttttgttac gcgaattaga aattttggca gcatcaaaaa
     1021 gctttgaaaa agtagataaa cgcttttttg gcttgcaacc aacagaagag ggagtcgaag
     1081 tagcgcaagg taagactatt gaagatattg gcgatattac tgccttagaa gaaagcttgc
     1141 agcaatcaga ccgatattta gcaatcagtt ggcactaaga cgacaaggaa tcaattcatg
     1201 acaataaatg ttgatggact taaatttgac tttgagacaa attggcaagc gagtaaatat
     1261 gatgaatgga gctattaccg caatcaattt gttaagcaat ttgatggcat taaagcggtt
     1321 gatttgttgg caatttcacc tgataaaatt acgtttttca tagaagtgaa agactatacg
     1381 catcctgata ccgagaaacc gtctaattta ccgcaagccg ttgctgataa agtgtttgac
     1441 acattagcag ctttattacc atgtagtttg cttgccaatg atgcaaatga aaaagccttt
     1501 gccaaaagag taatacaaag caagcagtta tgcgttgtct tgcacatgga acaaccacga
     1561 gcgcataggc cagtggttga ccctgccgat attttgcaaa aacttaaaaa attattaagg
     1621 gctattgatg cccatccaaa agttgtttca atacaaaaca acccattatt accttggact
     1681 gtgagtaagt aatgagccta aaaccttggc gagaaattgc tcgtccacac aaagatgttc
     1741 ttgagggaac ttttaaacaa tcagagtttg ctgccgacat tacgcaagtt gctaacggca
     1801 cagccactcc tgaatatcaa gatgccgaaa agttttttaa tcgcaccttt attacagaag
     1861 ggatgcgctt actactgatt tcggttgcac aacgtctttc tgggctgggt ggcgaccctg
     1921 ttattcaact gcaaacagca tttggtggtg gtaaaacgca taccatgctg gcggtctttc
     1981 acttagcaac acgcaaagtc agcaccgaaa aactattggg cgttggccct ctgctagata
     2041 aagcaggcat ctttgaacta ccacaagcgc gtgttgcggt aattgatggc attaaattat
     2101 cacctagcca accgcgtaaa tatggcacag tgcaaattaa taccttatgg ggtgagcttg
     2161 catggcaatt gctaggtaat gagggatata ccttagttgc agccagtgat attgatggaa
     2221 catcgccagg caaagaaatt cttactgagc taattcgtaa agcagcaccc tgcgtgattc
     2281 ttattgatga gctattggct tatgttcgtc aacttgagtt agggcagcat tacaaaggcg
     2341 gaacatttga tagtaatatc acctttattc aagcactcac cgaagccatg aaggctgttc
     2401 ccaatagcat cttactcgca tcactacctc aatccgaact ggaagtgggc ggcactatgg
     2461 gacaacgcgc tttaaactcc ttagagaagt attttgctcg ggtggaaacc gtttggaagc
     2521 cagtcgccac ggaggaagcc tttgagattg tgcgtcgtcg tctgtttgag tatccaggtg
     2581 atcgtgcgga agttgaaggc attagccgtc aattttcaga tttttatcgc aaacatgccg
     2641 aaaagtttcc tgtagaaacc cagtcaaatg aatattttga gcgactttgt cattcctatc
     2701 ccattcaccc agaaattttt gataggcttt atgaggattg gtcaacactc gataaatttc
     2761 aacggactcg tggtgtgctt caatatatgg caattgtgat acatcgcctc tggaactccg
     2821 aaaatcgtga tgccttgatt atgcctggct ccattccctt agaagatggt aatgtgcgag
     2881 ataagagtat tcactatctt tcaccaggct gggaacctgt gattgagcga gaagttgata
     2941 gcattcgttc cattccgcac gacatagaaa gtcacagtac attatttggt ggcgttcaag
     3001 cagcgcgacg ttcagcccga acaatatttt tgggtagtgc gccttcaaat agtagccaga
     3061 tagtacgcgg tattaatgtt gagcatattt tgctaggcgc agtacaacct ggccaatcag
     3121 taggtatttt tgaagatgtt ttaaaacgtc tgcgagaccg tttgcattat ttatattccg
     3181 atcaaggccg tttttggtta gacaccaaac ccaatctacg ccgagaaatg gaaagtcgaa
     3241 aagcaactat taatgaacga gatgagttga taccgttact caaaaatcaa gtcacagaaa
     3301 tttttggcaa aaatcatcat ttttcaggca ttcatgtttt tacgccatcg gttgatattc
     3361 ctgatgacta tggaatcggc acgcgattag tggtgttgcc tcttaatgca ggatacagcc
     3421 gtagcgaggt taattcagcc tttacagcag caacagaaat tttacgaaat cgcggtgaac
     3481 agccacgaca aaaacaaaat agattaattt ttatggccgc tgattttgat gttgtcagtc
     3541 gtttaaaaga gcaaggtcgt atttatttgg catggaaatc cattgttttt gatattgata
     3601 acggcactct caatcaggat ttggcacatc ttaatcaagc caaacgtaac cgcgatggcg
     3661 caattcaaac attaaaacaa ttggtacgag aagcctataa atggttgttg gcacccattg
     3721 aatcgtttgt gaatggcaaa ccaaatttaa cttgggaagc tgttgctctt tcgacaacag
     3781 caccaaattt tattcaagaa gttgaaagtc gcctgcggga agaggagtgg gtcatatttg
     3841 agtggtcgcc tattcattta cgcaatatgt taaaacagtg gtatttgaaa aacgatgtcc
     3901 aagaagtgaa tgcgctaaaa gtatggcaag atacttgtca ttacttatat ttgccgcgtt
     3961 tagtcaatga tcaagtattt aagaacgcca ttgctcaagg ggtttcaagt caggattatt
     4021 ttgcttttgc ctctgctaaa gaggagagcg gacgttattt aggattcagt tttggccata
     4081 atgttatttc aacattagat ggttcgtctc tgctaattga tcgtgaagct gcattggcat
     4141 atttggagcg aatacgtcct gtgccacaac cagcgttaga tgaaacctct agtgagccta
     4201 tagttattaa acctacaggt agcactccat cagtggcacc tgtatccgat cctcgtcctg
     4261 tggccacatc tgctaaaaca aaaaaacaat tttatggatc tatttcttta gacccagtga
     4321 aagctaagat ggaatttgct acgattatcg acgaagtgat tcagcaattt acgtcacgac
     4381 tcggtgtaga tgtcaccata tcggttgaag tccaagccaa tagttcgatg ggttttgatg
     4441 ataatttgca acgcgccata aaagaaaact gtaaggtact caaatttaat actgctgaat
     4501 ttgaggaaga ataaagcaga tactgcgtta ctattaagta acgcagtatc tctattcgac
     4561 ttaggggagt taccatgttc gtgtagaaat gataagtgtt gatattgtag gtcaattttg
     4621 gtacaaaatt tgtgtcaact acctcctgtt agcacatttt tgatatggtg gccatggttg
     4681 atagaaagtt gtgttaagtg ttcttgtgaa agtaacaatc gctaaaaatc aaatatatta
     4741 aaaggatgac tatgaatgca taacacagag cagatatttc taacaattgc caaataatag
     4801 actaatggaa aattcaattt attaggtgtg ttagtctgaa attagctttt tatcaaaaaa
     4861 ttagtattac tctaactacc tgttaatttc ttctaaagta ttaaatatgt taaaagtacc
     4921 attatacaaa tcttacattt attggcctct aattttctta gttgtgacga tactcacggc
     4981 tattaataac tatgcttttt atgaaactcg tgaaaaaa
//
LOCUS       DAOQMB010000145         5016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7740_length_5016_cov_13.100383, whole genome shotgun sequence.
ACCESSION   DAOQMB010000145 DAOQMB010000000
VERSION     DAOQMB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7740_length_5016_cov_13.100383"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="PLY05_06225"
     CDS             complement(<1..890)
                     /locus_tag="PLY05_06225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNC02936.1"
                     /translation="MQNSTWRSELVALKTLMAPILITQLAQAGYGFIDTVMAGQVSPL
                     DLSAVAIGSSVWLPLFLLISGIVMATTPLVAAAYGAKQYSEIPRITHQALWLGLGIGL
                     LGMWVIQHISFIFPLLDVPAHLQAKTELYLQGVSFGMPAVAIFCVLRFYSEALGHTKP
                     IMLISLLGILLNIPANYAFIYGWWFIPKMGGAGCGWATAIVMTTMMLVLLAYVLWSNA
                     FKQTRLLQHYFKPHLATIWQISKLGVPIGAAIFFEVSLFSCIAVLVSPLGELFVAAHQ
                     VSLSVTSMLFMVPLSLAISMT"
     gene            complement(890..1636)
                     /locus_tag="PLY05_06230"
     CDS             complement(890..1636)
                     /locus_tag="PLY05_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02937.1"
                     /translation="MHKKTELYTFETKNTIALQFNHDAIQSVMRKSDPFALEFEYTQA
                     MMAFLPLYEQIDHLLLIGLGGGSLSKFCYKQLPDSKITTVEISAEVISHRQLFQVPAN
                     NKRFKVLEADGAIYVQNKVDFCQVILLDAYDGEGMPDAFDEQFYQYCYQALHEQGVLV
                     VNLWRKDAYFRRNLDRIHRSFNEKTITLKCQSGNEIVFAFKNKQLAEFDEVWKQALIW
                     QKQTQINFPQFLEELVLSPKNTWFYNGRAD"
     assembly_gap    1768..1867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1905..2768
                     /locus_tag="PLY05_06235"
     CDS             1905..2768
                     /locus_tag="PLY05_06235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC02938.1"
                     /translation="MSNYASTVTPVGTANIPVRRMDFEFDDAIPKYWFDNNPMLTMLL
                     TGLSCVFPEGERMFMRAVRHYQDQVTDPVLQKEIRAFIGQEAHHGKEHQAFNEFMARK
                     GLPIAEIEQFVKQGIAFEEKHLSKQRMLAKTCALEHFTAMFAEMILEHPEFLDGIDER
                     LKPLWLWHAIEESEHKAVAYDVYQTTVGNYWTRTSEMAATTILFSFFTGLHTVQLLRA
                     SGEQRNIKAIAKGLNKLIGRKGFFRGMLPHYLAYYSPNFHPNQRDSTELREKGLTLLQ
                     QYLGDKKSLAA"
     gene            2845..4377
                     /locus_tag="PLY05_06240"
     CDS             2845..4377
                     /locus_tag="PLY05_06240"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC02939.1"
                     /translation="MSAAQFPERHIFTPIVIVGSGFAGIGTAIRLQQKGFNDFLMLEQ
                     ASNFGGTWRDNTYPGAECDVPSHLYSFSFEPNPHWSKAFAPQQEIQKYLLHCAQKHKL
                     ASKALFNAEVVNACFNEAKGEWTVTLADGCTVRCRLLIMGNGGLSKPSYPVIEGQQSF
                     QGKIMHTARWHESFDYAGKKVAVVGTGASAIQLIPELAKTAAQVVVFQRTPAWVMPKW
                     DTHFSAHLQQEFADHPNLRKALRYGIYWGAEAMSMALIWNSPLAKSMQWLGELHLKTI
                     KDNTLRTKLTPDYKVGCKRMLISNDYYPAMQRDNVQLVTNGIKKIDSTGIHSQLGEHY
                     PVDAIVYATGFQVNVAGSPFPITGLDNRQLMTEWTTGSEAYKGININGYPNLCLLMGP
                     NTAAGHTSVLVYLEGQIEYVLNYTKTLFDLSFKYLDIKKSAQDSFNKMIQRRMKYTVW
                     TTGCQSWYLTKEGKNSTLWPGFSWEYRLRTAFFNHDDYERVGAKYQKGVRTKKQKTIT
                     QS"
     gene            4427..>5016
                     /locus_tag="PLY05_06245"
     CDS             4427..>5016
                     /locus_tag="PLY05_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02940.1"
                     /translation="MNTWLSSSQNARFLSFVLVAIGFIVAVKLGLLVPIYAGLLAFCL
                     VTRFSDKIVDERIRSIRSKWIATSLVTALVVLVLVGAGAGIHAMLKATTDVHELMIKM
                     SEILHSARSWLPEKISNAIPQQSDLLTKLSDWLRTHATEIGTFGLGALKGIGLALFGV
                     LLGALIAVSDATRSSSFGVVTQNLLNQVVALRESFWR"
BASE COUNT         1538 a          901 c         1014 g         1463 t
ORIGIN      
        1 gtcatgctaa tcgctaaact taatggcacc ataaacagca tcgaagtgac tgataaactg
       61 acttgatgcg cagcgacaaa taattcccct aaagggctaa ctagcaccgc aatacacgaa
      121 aataaactca cttcaaaaaa aatagctgca ccaattggta cacccaattt gctaatttgc
      181 caaatggtgg ctaaatgggg tttaaaataa tgctgtaata aacgagtttg tttaaaagca
      241 tttgaccata acacataagc caacagaacc aacatcattg tggtcatcac aatagcggtt
      301 gcccaaccac aacccgcacc gcccatttta ggaataaacc accagccata aataaatgca
      361 tagttggcag gaatgtttaa taaaatccct aaaagactaa tcaacataat cggtttggtg
      421 tggcctaaag cttcgctata aaatcttaaa acacaaaaaa tggctaccgc aggcatacca
      481 aaagacacac cttgtaaata cagctcagtt tttgcttgta aatgggcggg cacatctaat
      541 aaaggaaaaa taaaactaat atgttgaata acccacattc ccaacaaacc aatgcctaaa
      601 cccaaccata atgcttggtg agtaatgcgt ggaatctcgc tgtattgttt tgcaccataa
      661 gccgcagcaa ctaaaggggt agtggccata acaatacccg aaatcagcaa aaataacggc
      721 agccaaacac tagaaccaat ggccactgct gataagtcta agggggaaac ttggccagcc
      781 attactgtat caataaaacc ataacctgct tgagcaagtt gagtaattaa aatcggcgcc
      841 attagggttt tgagtgcgac taattcacta cgccatgtag agttttgcat taatcggcac
      901 gcccattata aaaccatgta ttttttggac ttaaaaccaa ctcttctaaa aattgtggaa
      961 agttaatttg agtttgtttt tgccaaatta acgcttgctt ccatacctca tcaaattcgg
     1021 ctaactgttt atttttaaag gcaaagacaa tttcgttgcc actttggcat tttaaggtaa
     1081 tggttttttc attaaagcta cggtggatgc ggtcaagatt acggcgaaaa taagcatctt
     1141 tacgccacaa gttgacgact aacacacctt gttcatgtaa cgcttgataa cagtattggt
     1201 aaaattgttc gtcaaaagca tcaggcattc cttcgccatc ataagcatcc aataaaatta
     1261 cttggcaaaa atcaactttg ttttgcacat aaattgcacc atcagcttct aacactttaa
     1321 agcgtttgtt attagcgggt acttgaaata attgccgatg actaatcacc tctgcgctga
     1381 tttcaacggt ggtgattttg ctatcgggta attgtttata acaaaatttg gataaagagc
     1441 caccgcccaa accaattaat agtaagtggt caatttgctc atacaaaggt aaaaaagcca
     1501 tcattgcttg agtgtattca aactctaaag caaaaggatc acttttgcgc atcacacttt
     1561 gaatcgcatc atgattaaat tgaagggcaa tcgtgttctt ggtttcaaaa gtataaagtt
     1621 cagttttttt atgcataaga aatcaggttt ggcctaatat gacttgtaac ttggcacgat
     1681 agtctattac gttgaagggc agtttgcagg atactgcaaa catagacaca agtataacag
     1741 tttggactgg cgcgattatc gcttgttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnngtg aaatgcgtca gtcctcaaat ttcagaggtg taaaatgtca aactatgcaa
     1921 gcacagtcac tccagtaggt acagctaata ttccagtacg tcgtatggac tttgagtttg
     1981 acgatgcaat tcccaaatat tggtttgata ataaccccat gctgaccatg ttattaacag
     2041 gtttgtcttg tgtgtttcct gaaggcgaac gaatgtttat gcgtgcggtg cgtcattatc
     2101 aagaccaagt cacagaccct gtattgcaaa aagaaattcg ggcatttatt ggtcaagaag
     2161 ctcatcatgg caaagagcat caagcattta atgagtttat ggcgcgtaaa ggtttgccaa
     2221 ttgcagagat agagcaattt gttaaacaag gcattgcttt tgaagaaaaa catttgtcta
     2281 aacaacgtat gttggccaaa acctgtgctt tagaacattt tactgcaatg tttgccgaaa
     2341 tgattttgga gcatcccgaa tttttagatg gtattgatga gcgtttaaaa ccattatggt
     2401 tatggcatgc aattgaagag agtgaacata aagccgttgc ttatgatgtt tatcaaacaa
     2461 cagtaggtaa ttattggacg cgcaccagtg aaatggcggc aactacgatt ttatttagct
     2521 tttttacagg tttacatacc gttcaattat taagagcctc tggcgaacaa cgtaatatta
     2581 aggctatagc caaaggttta aataaattga ttggacgcaa aggctttttt aggggaatgt
     2641 tgccacatta tttggcttat tatagcccta attttcatcc taatcagaga gatagtactg
     2701 agctaagaga aaaaggctta actttgttgc agcaatattt aggtgataaa aaatcattgg
     2761 cagcttaatt gataagcgtt aatctacagg cgggagtttc ccgccttttt gtttttaaaa
     2821 gaataagaat taagagtgtt aataatgagt gctgcacaat ttcctgaacg ccatattttt
     2881 acccctattg tgattgttgg ttctggtttt gcgggcattg gcacagccat tcgtttgcag
     2941 caaaaaggct ttaatgattt tttaatgtta gagcaagcta gtaattttgg cggcacatgg
     3001 cgcgataata cttaccctgg tgcagagtgt gatgtgcctt cacatttata ttccttttct
     3061 tttgaaccca atccccattg gtctaaagcc tttgctcccc aacaagaaat tcaaaaatac
     3121 ttattacatt gtgcgcaaaa acataaactt gcttctaagg ctttatttaa tgccgaagtt
     3181 gttaatgcct gttttaatga agcaaaaggt gaatggacag tgactttagc tgatggttgt
     3241 acggtacgct gtcggttgtt aattatgggc aatggtggtt taagcaagcc aagctatcct
     3301 gtcattgagg gtcaacaaag ctttcaaggt aagatcatgc acacggcacg ttggcatgaa
     3361 agttttgatt atgcaggtaa aaaagtggct gtcgttggca caggtgcgag tgcgatacaa
     3421 ttgattcctg aattggccaa aacagcagcg caagtagtgg tgtttcaacg aactcctgca
     3481 tgggtgatgc ctaaatggga tacgcatttt tcggcacatt tacaacaaga atttgccgat
     3541 catcctaatt tacgcaaagc cttacgttat ggcatttatt ggggggcaga agccatgtcg
     3601 atggctttaa tttggaatag cccactggca aaaagtatgc aatggttggg agagttgcac
     3661 ttaaaaacca ttaaagacaa taccttgcgt acaaaattaa ccccagatta taaagtgggc
     3721 tgtaagcgga tgctgatttc taatgattat tatccagcga tgcagcgtga taatgttcag
     3781 ttagtgacca acggtataaa aaagatagat agcacaggca ttcatagtca attaggcgaa
     3841 cattatcctg ttgatgccat tgtgtatgct acaggttttc aagtcaatgt tgcaggtagc
     3901 ccatttccta tcacaggttt agataatcgc cagttaatga ctgaatggac aacaggtagc
     3961 gaagcctata aaggcattaa tattaatggt tatcctaatt tgtgtttatt gatggggcca
     4021 aatacggcag cagggcatac ctcagtatta gtctatttag aagggcaaat tgaatatgtc
     4081 ctcaattaca ccaaaacctt gtttgatctt tcgtttaagt atttagatat taaaaaatcg
     4141 gcacaagata gttttaataa aatgattcaa cgccgaatga aatataccgt ttggacaaca
     4201 ggctgccaga gttggtattt aaccaaagaa ggaaaaaact caaccttgtg gccgggtttt
     4261 tcttgggaat atcggttacg tactgccttt tttaatcacg atgattatga gcgagtcggg
     4321 gcaaaatatc aaaaaggtgt gcgtaccaaa aaacagaaaa cgatcacaca atcttaacaa
     4381 ataatcatgt taagctgagt tttatttttt ttcatggact tagtgtatga acacatggtt
     4441 gtcctcatca caaaatgcgc gttttctgtc atttgtgcta gtggctattg gttttatagt
     4501 tgctgtcaaa ttagggttat tagtaccgat ttatgctgga ttattagcct tttgtttggt
     4561 tacgcgcttt tcggacaaaa tagttgatga gcggattcgc tcaattcgct caaaatggat
     4621 agcaacaagc ttggtcacag cgttagtcgt gttagtgttg gttggcgcag gagcaggtat
     4681 tcatgccatg ctcaaagcaa ccaccgatgt ccatgagtta atgattaaaa tgagtgaaat
     4741 tttacattca gcacgctcat ggttacctga aaaaatcagt aatgctattc cacaacaaag
     4801 tgatttatta acaaaattga gtgattggtt gcgtacccat gctactgaaa ttggcacctt
     4861 tggtttaggg gcattaaaag gcattggttt ggctttgttt ggtgtgttat taggggcgtt
     4921 aattgcggtc agtgatgcca cacgctcaag tagttttggc gtagtaacac aaaatttact
     4981 caatcaagtg gttgccttac gtgagtcatt ttggcg
//
LOCUS       DAOQMB010000146         4951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7899_length_4951_cov_15.806985, whole genome shotgun sequence.
ACCESSION   DAOQMB010000146 DAOQMB010000000
VERSION     DAOQMB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7899_length_4951_cov_15.806985"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="PLY05_06250"
     CDS             complement(<1..740)
                     /locus_tag="PLY05_06250"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC02941.1"
                     /translation="MSKSLPTALAQWQAQGSYATFVGYNVFYRDFGTKHSDAILLLHG
                     FPSSSYDWHTLIPLLGDEKRIVCLDFLGFGLSDKPDQHSYSLMEQADIVELLLKQLGI
                     KRVKIIAHDMGASVTCELLARRERKLLGFNVQSVLLMNGSVYIDLAQLTPSQKLLRSP
                     FAEFFAKISLKPIFMLQLQKILAQKISKQELEMMWASLVYKQGKERLAQTISYIDERY
                     RFAHRWLPPLTRLDIPVLILWGQKDPVAV"
     gene            complement(751..2253)
                     /gene="ppx"
                     /locus_tag="PLY05_06255"
     CDS             complement(751..2253)
                     /gene="ppx"
                     /locus_tag="PLY05_06255"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HNC02942.1"
                     /translation="MNRQLTEGSLLAAIDLGSNSFHLAIARLDHGEVRRIDSLSEKVQ
                     LGAGFDKDKNLTLEAQARALACLARFAQRLQAIPLRYLRIVATNALRQANNSAQFIEQ
                     AEHILKKRIEIVAGREEARLIYLGVSHTLAEEGRRLVVDIGGGSTEFIIGENFEPLAT
                     ESLQMGCVAYTQRFFADGSISAKALDKAIMAARQEVMAIANAYRDLGWHSVVGSSGTI
                     KATRQILVQNGWASPEGFITKEGLAKVRDVVLACKHVSELNVVGLKDDRRAILPAGYA
                     IIQAVFDELNLETMNYSDGALREGVLYDMLGRFSHEDIRDRSANALMARYHVDTAQAQ
                     RVAQTAENFYQQLHSSLTIDEEDEDLLRRAALLHEIGLAISHSSYHKHGAYLLQYSDV
                     AGFSQAEQERLALLVACHRRKIRNEQKVQLMSKGGTSLFNIAVILRLAALVHHSRSPE
                     PLPDLKLSSDGLHFIMQFPDGWLASHPLTAADIEEEKDYLQAWDISLVAS"
     gene            2443..2772
                     /gene="trxA"
                     /locus_tag="PLY05_06260"
     CDS             2443..2772
                     /gene="trxA"
                     /locus_tag="PLY05_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008073352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HNC02943.1"
                     /translation="MSNPLIVNTTDAQFEADVISAEGLVLVDYWAPWCGPCKMIAPIL
                     DDLAGEYEGKVKIVKVNVDDNPASAAKYGVRGIPTLSLFKGGQVVATKVGALAKSQLT
                     AFLDSNI"
     assembly_gap    2907..3006
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3007..4059
                     /gene="rho"
                     /locus_tag="PLY05_06265"
     CDS             <3007..4059
                     /gene="rho"
                     /locus_tag="PLY05_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HNC02944.1"
                     /translation="SYLAGPDDIYVSPSQIRRFNLRTGDTIAGTIRPPKEGERYFALL
                     KVNHINYDSPENARNKILFENLTPLFPTERLVLELGNGSTEDLTARVIDLVAPVGKGQ
                     RSLIVAPPKAGKTILLQNIAHSIARNNPEVHLIVLLIDERPEEVTEMIRTVRGEVVAS
                     TFDEPPARHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGG
                     VDAHALERPKRFFGAARSIEEGGSLTIIATALVETGSKMDEVIFEEFKGTGNHEVHLD
                     RRIAEKRVFPSINIKKSGTRREERLMADDELKKVWILRKILHPMDEIAAIEFLLDKMK
                     DTKTNDDFFEAMKRKQ"
     gene            complement(4174..4371)
                     /locus_tag="PLY05_06270"
     CDS             complement(4174..4371)
                     /locus_tag="PLY05_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02945.1"
                     /translation="MTMVTLDVSRVDAKEIHQQILQLQAMQHDMLDRDSVAANTVAST
                     LKLLHRLEDELRHQARVDHHH"
     gene            complement(4442..>4951)
                     /gene="msrA"
                     /locus_tag="PLY05_06275"
     CDS             complement(4442..>4951)
                     /gene="msrA"
                     /locus_tag="PLY05_06275"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNC02946.1"
                     /translation="GGGCFWCLEAVFQRVKGVQQVTSGYAGGARANPSYQQICTGATG
                     HAEVVKIDFDANIISFEQLLTIFFAIHDPTTLNRQGNDIGTQYRSVIFCQSAEQRQIA
                     TAFIAKLNQEKVFSNAIVTQISDAVPFYAAEAYHQNYFNNHPQQGYCQFVVTPKVLKF
                     QQYFKAYHS"
BASE COUNT         1427 a         1016 c         1034 g         1374 t
ORIGIN      
        1 acagccacag gatctttttg tccccacaaa ataagtacag gaatatccaa acgggttaat
       61 ggcggcaacc aacgatgggc aaaacgataa cgctcgtcaa tataactaat ggtttgtgct
      121 aaacgttctt tgccttgctt ataaaccaaa ctcgcccaca tcatttctag ttcttgtttg
      181 ctgatctttt gggctaagat tttttgtaat tgcagcataa aaatcggctt gagcgaaatt
      241 ttggcaaaaa actcagcaaa gggcgaacgt aacagttttt gagagggcgt aagctgtgct
      301 aaatcaatat aaacgctgcc attcatcagt aataccgatt gcacgttaaa acctaataat
      361 ttgcgttcgc ggcgtgctaa taactcacag gtgacactag ctcccatatc atgagcaata
      421 attttgaccc gtttaatacc taattgtttt agcagcaact caacaatgtc tgcctgctcc
      481 attaaggaat aactgtgttg atcgggtttg tcagacagac caaagcctaa gaagtctaaa
      541 cacacaatgc gtttttcatc gcctaaaagc ggaatcaaag tgtgccaatc ataagagctt
      601 gacggaaaac cgtgtagcaa cagaatggca tcactgtgtt ttgtgccaaa atcgcgataa
      661 aaaacattgt agccaacaaa agtggcataa cttccttgtg cttgccattg ggcaagtgct
      721 gttggcagcg attttgacac aatcattctc ctagcttgca actaaactaa tatcccatgc
      781 ctgtaaataa tccttttctt cctcaatatc ggcggctgtt aaggggtggc tggctaacca
      841 accatcggga aattgcataa taaaatgcaa accatcgctg cttaatttga gatcaggtaa
      901 gggctctgga ctacggctat gatgtactaa tgccgctaga cgtaaaatca ccgcaatgtt
      961 aaataaagat gtaccacctt tactcattaa ttgcactttt tgttcattac gaattttgcg
     1021 gcgatgacaa gctactaata gggctaaacg ttcttgctct gcctgagaaa aaccagccac
     1081 atcagaatat tgcaacaaat aagcaccatg tttgtgatag ctgctgtgtg aaatggctaa
     1141 gccaatttcg tgtaataaag cggctcgacg caataaatct tcgtcttctt catcaatcgt
     1201 taaagagcta tgtaattgtt gataaaaatt ttcggcagtt tgcgctacac gttgagcttg
     1261 agcggtatcc acgtgataac gagccattaa tgcattggcg ctacggtcac gaatgtcttc
     1321 atggctaaaa cgacctaaca tgtcatagag tacgccttca cgtaatgcac catcagaata
     1381 gttcatggtt tcgaggttta actcgtcaaa tacggcctga ataatcgcat aacctgcggg
     1441 taaaatggcg cggcgatcat cttttaaacc aacgacattt aattcgctaa cgtgtttgca
     1501 agccaaaacc acatcacgaa ctttggctaa gccttctttg gttataaaac cttcagggct
     1561 tgcccaacca ttttgtacca aaatttgccg agttgctttg attgtgccac tagaaccgac
     1621 cacagaatgc cagcctaaat cacggtaagc attagcaatg gccatgactt cttggcgtgc
     1681 ggccataatc gctttgtcta aagctttggc actgatgctg ccatcggcaa aaaaacgttg
     1741 ggtataagcc acgcagccca tttgtaagga ttcggtggct aaaggctcaa aattttcacc
     1801 aataataaat tcggttgaac caccgccaat atcaaccacc aaacgacggc cttcttcggc
     1861 gagggtgtga gagaccccta aataaattaa ccgtgcttct tcgcggccag caacgatctc
     1921 aatgcgtttt ttgaggatat gttcggcttg ttcaataaat tgtgcggaat tatttgcttg
     1981 gcgtagggca ttggtggcga caatacgcaa ataacgcaaa ggaatggctt gtaaacgttg
     2041 ggcaaaacgc gccaaacacg ctaaagcgcg tgcttgtgct tctagggtga gatttttatc
     2101 tttatcaaaa cccgcgccta attggacttt ttcggacaaa ctatcaattc ggcgtacttc
     2161 accatgatct aaacgggcaa tggctaaatg aaaactattg gagcctaaat caatggcagc
     2221 aagcagacta ccttcagtca attgtcgatt catgaaatac ctgtttgatg agttggagcg
     2281 aatagacaaa attgccaatg gttacaaacc catgtgacaa acgcttgaaa aatggctttt
     2341 ttcgccccat aaattgtaaa tacacttttg tataagatat aacagctttg gtcgtataaa
     2401 aaaagtctgt tattatcgca ctttatttat ttggagaatc ccatgagcaa tccattaatt
     2461 gtcaatacca ctgatgccca atttgaagct gacgtgataa gtgccgaagg tttagtatta
     2521 gttgactact gggcaccatg gtgtggtcct tgcaaaatga ttgctccaat tttagacgac
     2581 ttggctggcg aatacgaagg caaggtaaaa attgttaaag taaacgtcga tgataaccca
     2641 gcaagtgctg caaaatacgg tgttcgtggc attccaacct taagtttatt caaaggtggt
     2701 caagttgttg caacaaaagt gggtgctttg gcaaaatccc aattgacagc cttcttagat
     2761 tctaatatat aatatacacc caaggcggcc aagtactttc taataggtcg cctttcgtcc
     2821 cgcgcagccg ctctgttagg catcctattt tataactctg tctacagcgc aactgatatt
     2881 ccccaagatt aatcaggact tatgcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnntctt atttggctgg ccctgacgat atttatgtca gtccttcaca aattcgtcgt
     3061 tttaacctca gaacaggtga cacgattgca ggtacaattc gtccacctaa agaaggcgag
     3121 cgttattttg ccttattaaa agtgaatcat attaactatg attcacctga aaatgcccgt
     3181 aacaagattt tatttgaaaa cttaacgcca ttattcccaa cagagcgttt agtccttgag
     3241 ttaggtaacg gctctaccga agatttaact gcccgtgtca ttgatttggt tgcgccagtg
     3301 ggtaaaggtc aacgttcgtt gattgttgca ccgcctaaag caggtaaaac cattcttttg
     3361 caaaatattg cgcactcaat tgcccgtaac aatccagaag ttcatttaat tgtattatta
     3421 attgatgaac gtcctgaaga agtgacagag atgattcgta cggtgcgagg cgaagtggtt
     3481 gcttcaacct ttgatgagcc gccagcccgt cacgttcaag tagcagaaat ggtcattgaa
     3541 aaagccaaac gtttggttga acacaaaaaa gatgtggtga ttttattaga ttcgatcacc
     3601 cgtttagcac gtgcctataa caccgtcatt ccttcatcag gtaaggtgtt aacaggtggt
     3661 gtggatgccc atgctttaga acgtccaaaa cgtttctttg gtgcagcaag gtcgattgaa
     3721 gagggtggct ctttaaccat tattgcgact gctttggtag aaactggttc taaaatggac
     3781 gaagttatct ttgaagaatt caaaggtaca ggtaaccatg aagttcactt ggatcgtcgt
     3841 attgccgaga aacgtgtatt cccatccatc aacattaaga agtcaggtac tcgtcgtgaa
     3901 gagcgcttga tggcggatga tgaattgaag aaagtatgga ttttacgcaa aatcttacat
     3961 ccaatggatg agattgccgc gattgagttc ttgttggata aaatgaaaga caccaaaacc
     4021 aatgatgact tctttgaagc catgaagcgt aaacagtaaa attttaaccc tcactctagc
     4081 tctctcccac agggagagag aacattgcct tgcgtttgga gtgatggcaa aaacaagacc
     4141 tcgtgctagg cagctagcac gaggtttttt attttaatgg tggtggtcaa cacgagcctg
     4201 atgacgcagt tcatcttcta ggcgatggag tagttttagg gtgctggcga cagtgtttgc
     4261 agcaacgctg tctcggtcaa gcatatcatg ttgcatggct tgaagttgta aaatttgttg
     4321 atgaatttct ttggcatcaa cacgactaac atctaaagtc accatcgtca tttgggcatc
     4381 tcctgtgcag attatgggta ctacacaaga tatagacgaa gaatgtaggg tttgccagtt
     4441 tttaactgtg atatgcttta aaatattgct gaaacttcaa cacttttggc gtaaccacaa
     4501 actgacagta gccctgttgt gggtggttat taaaatagtt ttgatggtag gcttcggcag
     4561 cataaaaagg cacagcatca ctaatttggg tcacaatggc attgctgaaa actttttctt
     4621 ggttaagttt ggcaataaaa gccgtggcta tttgtctttg ctcggcagat tggcaaaaaa
     4681 tcactgagcg atattgggtg ccaatatcat tgccttgacg gtttaatgtg gttgggtcat
     4741 gaatggcaaa aaagatagtt aacagttgtt caaagctaat tatattggca tcgaaatcaa
     4801 ttttaaccac ttcggcatga cctgttgcac cagtgcaaat ttgctgataa gatggatttg
     4861 cccgcgcacc gcctgcataa cccgatgtca cctgttgcac acctttgacc cgttgaaaca
     4921 cggcttctag acaccagaaa caaccaccac c
//
LOCUS       DAOQMB010000147         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_7936_length_4937_cov_19.536051, whole genome shotgun sequence.
ACCESSION   DAOQMB010000147 DAOQMB010000000
VERSION     DAOQMB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7936_length_4937_cov_19.536051"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1090
                     /locus_tag="PLY05_06280"
     CDS             113..1090
                     /locus_tag="PLY05_06280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HNC02947.1"
                     /translation="MKAILCKTHGLPETLVLEDIPSPQAGAKQVVISVKACGVNFPDT
                     LIIQNKYQFKPELPFSPGGEVAGVVKEVGEGVKHLKVGDTVIAMTGWGGFAEEVVTDG
                     SRVFPVPPVFDFKTAASFAYVFGTSYHALKDRAQLQAGETVLVLGAAGGVGLAAVQLA
                     KVMGAKVIAAASTAEKLAVCQQYGADAVVNYTEANWREQIKTLTDGKGVDVVYDAVGG
                     DYAEPALRSMAWRGRYLVVGFAAGDIPKVPLNLALLKGCAIVGVFWGEFAVRQKQDNQ
                     ANMMQLFAWLMQGQLKPHISAEYPLAQAAQALNDLMQRKVTGKAVLVME"
     gene            complement(1147..1392)
                     /locus_tag="PLY05_06285"
     CDS             complement(1147..1392)
                     /locus_tag="PLY05_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HNC02948.1"
                     /translation="MAHIKQFISTNRYTLKKTLSYYVMHITVAAIVAFMVTGNIWMAL
                     TLSMLEPSVQAVAFFFHERAWARKEQTTIAIPTVVHA"
     gene            1615..4863
                     /locus_tag="PLY05_06290"
     CDS             1615..4863
                     /locus_tag="PLY05_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HNC02949.1"
                     /translation="MDKPSTSLLRTIIAHPLFGNLLLACLYFLTGHIGLALAAAPNFA
                     TAVWPASGIALAWVLMFGWRFAIGVALGSFVVSSLETAKALELSVFEINHLIPLAIAC
                     GAAAQAILGAWLIKRYANYPNPLDTPVNVFKLLLLGGVIATLLNATWSVSVLYFSKAI
                     SQDFVLQSWFVWWVGDSIGVLVFAPMLLALGSDSPFFNKTRAVLVAFVSVTTFALAAL
                     IFLLAVQLEHKDRLAEFKAHTQQLMQQLQQRLQEYESFALFAQGFFAGSEFVSQEEFY
                     RFSKSWLQKHNEVQAVEWAPKVLLSQRSQWEKQQSELLKKAVLITERGGTQRDAVLMT
                     ARERELYFPILYIEPWQKNQQMLGYDTQSNEGSADLLKHIMSTELPLLSSPLKLIQSP
                     TKESGVLLYVPIFQPDVARSIDSLKGVLILVLKNEMVLAKVHQELIGKSFLLLVRDQH
                     SKQSIYGIEPSAAELKKVSSLQMYYQGKVSFGQRLWIVDIWPSGQKLASYQSWVTWLV
                     LVIGLLSTSLAVSYTLISTGYRQSLEKAVKQQTDTLVRQNQQLELARQDADKANAAKS
                     LFLANMSHEIRTPMSGVLGMTELLRDTSLNMQQQQYLQVIYASGKAMLSIINDILDYS
                     KIEAGKMEVEQIDVDLGEVLLECSSIFYLVAEQKGIDFIVSVEANTPTLVQTDPTRLK
                     QILLNLLSNAFKFTTNGQVSLRIYQRYSQDSWLYIEVADTGIGISAEQKHSLFDAFQQ
                     ANLSTTRQFGGTGLGLSISKQLAELMNGQIVVESELAKGSRFIVALPYVMATEQFKEQ
                     HRQASSLLKGLRVLLVDDNNDFGRHIVQQAELWGIHIDIIHDGEYALLLLKRAMQGQR
                     PYHVVIFNVQQSTISIETLAEQIKQSPELQSLKRILVSRVATQPTAQQLADLAIDAVM
                     QKPHSFMPMRDAILSVIPVEQIDQRYGALSPTQSSILAKKHILLAEDYMVNQMVIVAM
                     LQKMHITVDVVSNGKEALTAYRQQPQAYALILMDCEMPEQDGYVTTQLIRQFEQQQQL
                     KAIPIVALTAYVKAEQYKHCLEVGMNDFLSKPLTAQTLKACLLKNIGVDKTA"
BASE COUNT         1462 a          882 c         1075 g         1518 t
ORIGIN      
        1 ccgcaattaa acacagtcaa ttggtggtga ttccacaagc agggcatacc tcaaccgttg
       61 aaacacctga tgcagtcaat gcagccttag aaaagtttat aaaggtaaca taatgaaagc
      121 tattctttgc aaaactcatg gtctcccaga aactcttgtt ttagaagata ttccttcacc
      181 acaagcaggt gcaaaacaag tcgtcattag cgtcaaagcc tgtggtgtta attttcctga
      241 taccttaatt attcaaaata aataccaatt taaacccgaa ttgccctttt caccaggtgg
      301 tgaagtggca ggtgtggtta aagaagttgg cgaaggtgtt aaacacctaa aagtcggaga
      361 taccgtcatt gccatgaccg gctggggtgg ctttgccgaa gaagttgtca ctgatggctc
      421 acgggtgttt cctgtgccac ccgtctttga ttttaaaaca gcggcaagtt ttgcttatgt
      481 ctttggcacg tcctatcacg cattgaaaga ccgtgctcag ttgcaagcag gtgaaaccgt
      541 attggtcttg ggggcagcag gtggagtggg tttagctgcg gtgcaactcg ccaaagtcat
      601 gggggcaaaa gtgattgctg ccgcctcaac ggctgaaaaa ttagcggttt gccaacaata
      661 tggtgcagat gctgtggtca attacaccga agctaactgg cgtgaacaaa tcaaaacctt
      721 gactgatggc aaaggggtgg atgtagttta cgatgcggtg ggtggcgatt atgccgaacc
      781 cgccttgcgt tcaatggcat ggcgtggtcg ttatttagtg gttggctttg cggcgggtga
      841 tattcccaaa gtgccactaa atttagcttt actcaaaggt tgtgcaattg ttggtgtgtt
      901 ttggggagag tttgcggttc ggcaaaaaca agataatcaa gccaatatga tgcagttgtt
      961 tgcatggctg atgcaagggc aattaaaacc gcatatttcg gctgaatatc ccttggcgca
     1021 agccgctcaa gccttgaatg acttaatgca gcgtaaagta acaggtaaag cggtattagt
     1081 catggagtaa agtaatcatc gactccgtgc tttgcatgga gtcgacatga tgactaagat
     1141 gcttagctaa gcatgaacaa ccgttggtat ggcaatggtt gtttgctctt tacgcgccca
     1201 tgcccgttca tgaaagaaaa aagccaccgc ttgcacacta ggctccaaca tactcaacgt
     1261 taacgccatc caaatattgc ctgttaccat aaaagcgaca atcgcagcga cagtaatgtg
     1321 catcacatag taacttaagg tcttctttaa ggtataacga ttggtgctaa taaattgttt
     1381 aatgtgagcc atgatcgtat cctctaggtt atgctttcat agactccatc ttagagagaa
     1441 aaaattattg tgtaaaacta ttaattttta ttaatttaat agtttttata tattgatgtt
     1501 atgttttaaa agttctacaa actctatttt gacagtcgga caatttacgg ctaataatat
     1561 atttatatta aatttcaatc tattggtaat tgttttctag ggatatagga atttatggat
     1621 aaaccatcca cctcattgtt acgtactatt atcgctcatc ccttatttgg taatttactt
     1681 ttagcctgtc tttatttttt aacggggcat attggtttag cgttagccgc tgcgcctaat
     1741 tttgcaacgg ctgtttggcc tgcatcgggc attgctttag catgggtatt gatgtttggt
     1801 tggcgttttg cgattggtgt tgcattgggg tcttttgtgg tatctagctt agagactgcc
     1861 aaagccttag agttgagtgt gtttgaaatt aaccatttga ttccccttgc aattgcttgt
     1921 ggtgcagccg cacaagcaat attaggcgca tggttaatta aacgttatgc aaattatcct
     1981 aatcctttgg atacgccagt taatgttttt aaattgttac tacttggtgg tgtgatagcg
     2041 accttattaa atgcaacatg gagtgtatca gtcttatatt ttagtaaagc aattagccaa
     2101 gattttgttt tacaaagttg gtttgtatgg tgggttggtg acagtattgg tgtattggta
     2161 tttgccccca tgttattggc cttaggttca gacagtcctt tttttaataa aacaagggca
     2221 gtgttagtag cttttgtttc ggtaaccacc tttgcattag ctgcattaat ttttttatta
     2281 gctgtacagt tagaacataa agatcgatta gcagagttta aggcacatac tcaacaattg
     2341 atgcagcaat tacaacaacg cctacaagaa tatgaaagtt ttgctttatt tgcgcaaggt
     2401 ttttttgcag ggagtgaatt tgtctctcaa gaggagtttt atcgtttttc taaatcatgg
     2461 ctgcaaaaac ataatgaggt acaagcggtt gaatgggctc ctaaagtatt attatcacag
     2521 cgtagtcaat gggaaaaaca gcagtcagaa ctcttaaaga aagctgtctt gattaccgag
     2581 cgtggtggta cacaacgtga tgccgtgtta atgacagctc gtgagcgtga attgtatttc
     2641 cctattttat atattgagcc ttggcaaaaa aatcaacaaa tgttggggta tgacactcaa
     2701 agtaatgaag gcagtgctga cctactcaag catattatgt caactgaatt accgttgtta
     2761 tcgtcaccgc taaaattaat tcaaagtcca actaaagaat caggtgtttt attgtatgta
     2821 ccaatttttc aaccagatgt tgcacgtagt attgatagct taaaaggcgt tttaatttta
     2881 gtgttaaaaa atgaaatggt tttggctaaa gtacatcaag agctaattgg caaatccttt
     2941 ttattattag tgagagacca gcacagcaaa caaagcattt atggcattga accgagcgcg
     3001 gcagaattaa aaaaagttag ctccttacaa atgtattatc aaggcaaagt ctcttttggc
     3061 caacgattat ggatagtaga tatttggcct tcgggtcaaa aattggctag ctatcaatca
     3121 tgggtcacat ggttagtatt agttattggt ttattaagta ctagcttagc ggtgagttat
     3181 actttaatta gcacgggcta tcgtcaatct ttagaaaaag cggttaaaca acaaacagat
     3241 accttagtca gacaaaatca acagttagaa cttgcacgtc aggatgcaga taaagccaat
     3301 gccgccaaaa gtctgttttt ggctaatatg agtcacgaga ttagaacacc gatgagtggt
     3361 gtgttgggaa tgaccgaatt attacgtgat acctctttaa acatgcagca acaacaatat
     3421 ttgcaagtga tttatgcctc tggcaaagcc atgctcagta ttattaatga tattttggat
     3481 tattctaaaa ttgaagcagg caaaatggag gttgagcaaa tagatgttga tcttggtgaa
     3541 gtattactag agtgttcttc tattttttat ttagttgccg aacaaaaagg cattgatttt
     3601 attgtgagtg tcgaagccaa tacacctacc ttagtacaaa ccgatcccac tcgtttaaaa
     3661 caaattttgt taaatttgtt aagcaatgct tttaaattta ccactaatgg tcaagtgtcc
     3721 ttgcgtattt atcagcgata tagtcaagat tcgtggttat acattgaggt agccgatacg
     3781 ggcattggta tttctgctga gcaaaagcac agcttatttg atgcctttca acaagccaat
     3841 ttaagcacca cgaggcagtt tggtggcaca ggactaggct tgagtattag caaacaatta
     3901 gccgagttaa tgaatggtca aattgttgtc gaaagtgagt tagccaaagg ttctcgtttt
     3961 attgtggcat tgccttatgt gatggcaaca gagcaattta aagagcagca tcgtcaagcc
     4021 tcatcattat taaaaggttt aagagtcttg ttggtggatg ataataatga ctttggccgt
     4081 cacattgttc agcaagcaga attatggggc atacacattg atattattca cgatggtgaa
     4141 tatgccttgt tattgttaaa acgagcgatg caagggcaaa ggccgtatca tgtggttatt
     4201 tttaatgtgc aacaaagtac cattagcatt gaaactttag ccgagcaaat caaacaaagt
     4261 ccagaactgc aatcgttgaa gcgtattttg gtgtctcgtg tggcaacaca acctacggca
     4321 caacaactag cagatttggc aatagatgca gtgatgcaaa aaccacactc ctttatgcca
     4381 atgcgtgatg ctattttaag tgtgattcct gtcgagcaaa tagatcagcg ttatggcgca
     4441 ttaagtccaa cacaatcgtc aattttagct aaaaagcata ttttgttagc agaagattac
     4501 atggtcaatc agatggtgat tgtcgccatg ttgcaaaaaa tgcacatcac agttgatgtg
     4561 gtgagtaatg gtaaagaggc tttaaccgct tatcgccaac agccacaggc ctatgccttg
     4621 attttgatgg attgcgaaat gcctgaacaa gatggttatg tcaccacaca attgattcga
     4681 caatttgagc aacagcagca actaaaagca attcctattg tggcattaac cgcttatgtt
     4741 aaagctgagc agtataaaca ttgtttagag gtcggcatga acgatttttt aagtaagcct
     4801 ctaaccgctc aaacattaaa ggcttgttta ttaaaaaata ttggcgtaga taaaactgcg
     4861 taatgcaatg ctgccatatt cataaccctc atctagccaa gataaaacat cattatcttg
     4921 gcgaacccct gcaacat
//
LOCUS       DAOQMB010000148         4911 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8004_length_4911_cov_7.476524, whole genome shotgun sequence.
ACCESSION   DAOQMB010000148 DAOQMB010000000
VERSION     DAOQMB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8004_length_4911_cov_7.476524"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..389
                     /locus_tag="PLY05_06295"
     CDS             147..389
                     /locus_tag="PLY05_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HNC02950.1"
                     /translation="MNMPIHTHINNQPTYKMLRMKQLIEKTGLSRSTLYDIMDVKSKR
                     YDPTFPRAIKLTEATVCWLESEVNAWLESKIKNSRG"
     gene            complement(386..637)
                     /locus_tag="PLY05_06300"
     CDS             complement(386..637)
                     /locus_tag="PLY05_06300"
                     /inference="COORDINATES: protein motif:HMM:NF017243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HNC02951.1"
                     /translation="MNTLGAEMMSTWTKIEPTRNCYRFYAISLANDLFNAYVVSCEWG
                     RIGAKKFRRKVVVFNSLEEAMTQMKYEESLRVKHHYQPA"
     gene            1204..4266
                     /locus_tag="PLY05_06305"
     CDS             1204..4266
                     /locus_tag="PLY05_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HNC02952.1"
                     /translation="MKTIRNACELQPKALEINVGDQIEQLDQLIHDTNGKEYFEKTFI
                     TDGMKILLSKGIARLAGKTNDTVFHLKQAMGGGKTHLMVGFGLLAKDAKLRQDLIATI
                     SYQSDFNIAKIAAFNGRNNPHTYFWGEIARQLQKEGLFREYWESGAKAPDEQAWLRLF
                     EGDEPVLILLDEMPPYFHYYSTQVLGQGTIADVITRAFSNMLTAAQKKKNVCIVVSDL
                     EAAYDTGGKLIQRALDDATQELGRAEVSITPVNLESNEIYEILRKRLFVALPQKSEIA
                     EIASVYGARLAEAAKAKTVERSAEALANDIESTYPFHPSFKSIVALFKENEKFKQTRG
                     LMELVSRLLKSVWESKEDVYLIGAQHFDMSISEVREKLAEISEMRDVIARDIWDSTNS
                     AHAQLIDINNHNNHAKQVGTLLLTASLSTAINSVKGLSESEMLQCLIDPNNQASSYRT
                     ALAELQKSAWYLHQTQEGRHYFSHLENLTKKLQGYAEKAPQNKIDELVRQRLEEMYKP
                     TTKEAYDRVLPLPEMDEAEAALKTGRVLLIISPDGKTPPGVVAKFFRELINKNNILVL
                     TGDKSSIASIEKAARHVYAVRKANELPVSHLQRKELDEKKAQYQQDFQTTVLSVFDKL
                     LIPSFKGEDVLRAKVLDNTYPSNQPYNGEQQIIKTLTADPIKLYLQITENFDALRTRA
                     ESLLFGSQDEARKTDLLDKMKQKTQMPWLHNKGFDALIREAAQRGVWEDMGNGYITKK
                     PKPKMTDVMISEDNLPDDFGTVQLKIDVTNAGNSPRIHFAEDGVVSESSPILKENVLS
                     TKALRVQFLAVDPTGKNQTGTPKTWTNKLTIRNKFNEQSRTVELFVAPKGNIKYTLDG
                     SEARNGIEYTQEIKLGNQEANIYVFAECDGIEEKRKFSFPKAGSTEVPIIKEKPAVLV
                     SQPPKRLDSSAKTYDGLKLAKEKNIEFEHVMLTAGSSPKVITLSLGEIRINATFIEAA
                     LAHLQTLLPPDAPLVMSFKKVYTHTGHDLEQFAKQLSIEIKNGEVEQK"
     gene            4263..4841
                     /locus_tag="PLY05_06310"
     CDS             4263..4841
                     /locus_tag="PLY05_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3780 domain-containing protein"
                     /protein_id="HNC02953.1"
                     /translation="MSNTIDFGASSEFGMHHFYVEIPVAPRDAICIYEDFGFDGNDAK
                     RETVECRLMLARELWSKIRDDARRDFNARLKAKKQSTGSWTTGRVRLDRFLGRELCVL
                     AWAAEHASPDECLVICQKWLALRPEERWWLYSKTAAEAGLANQTQRGWRKALYCALSD
                     GQNIKATQKVTPKSKKKDDEGLETFNLNLFDV"
BASE COUNT         1657 a          904 c         1027 g         1323 t
ORIGIN      
        1 gccgaaggac gtttgggcgt ggtttaattc atcattaacg ggggactgat taaaggaggc
       61 cgaggttcgc ctagtgtgag gtaatttttt ataggcatag ccgatgttat gtggagagcc
      121 gtctccacgt aactgaggac tcgataatga acatgcctat ccatacccac attaacaatc
      181 aaccgactta caaaatgttg cgtatgaagc aacttataga aaagacaggc ttaagccgtt
      241 caacgctata cgacatcatg gacgtaaaat ccaaacgcta tgacccgacc tttccgcgtg
      301 caatcaagtt gaccgaagcg acggtatgtt ggctagaatc agaagtcaac gcatggcttg
      361 aatctaaaat caaaaacagc aggggttaag caggttgata atgatgttta acacgcaatg
      421 actcttcgta tttcatttga gtcattgctt cctctaaaga attaaaaaca acgactttac
      481 gcctaaattt tttagcccca atacgacccc attcacacga cacgacatag gcattaaaca
      541 aatcattagc caatgaaatg gcataaaagc ggtagcagtt acgagttggc tcaattttag
      601 tccatgttga catcatctca gcacccaaag tgttcaataa atcatcaata aactttaaag
      661 cagatttttt atattttcta gcttaaataa ccaagcaagt gacacaaatt gtgtcacttg
      721 cttggttatc taggtggggg cgttatcagg ggttgagtag tttaatcaat tcactgggac
      781 tttgataaat agtttcatac aactcgataa actccatcac atcactccac tctcaccccc
      841 aacattgtga ctatcttctt cataaaaaca tctatggatt aaagtggagg aatttacgaa
      901 caattataag gagttgcccg attactagcc cgtgatttat cttaaacaat agcaagagcc
      961 atgttatatt gaggctagac caaagggtgt tattcaacag actttaggct gagttcagta
     1021 tttaaattag aatatgattg attttagaca ttcaagccat cagcgtactg tgatgtgtat
     1081 gagattttaa ataaagtaat aaaatgcgct tcgcacattg gacacagcct ttttaaaaat
     1141 gattaactaa aaattagctg tattagcttt taattagaag aagtttaagt ttaggagttt
     1201 tagatgaaga cgattcgtaa tgcgtgcgaa ttacagccaa aagccttaga gataaatgtt
     1261 ggcgaccaaa tagaacagct agaccaactg atacacgata ctaatggcaa agagtatttc
     1321 gaaaaaacct ttattactga tggtatgaaa attttattgt ctaaaggcat tgcgcgtctt
     1381 gcaggcaaaa caaatgacac agtctttcat ctaaaacaag caatgggtgg tggtaaaact
     1441 cacttaatgg ttggctttgg attacttgca aaagatgcaa aacttagaca agacttaata
     1501 gcaacaatta gttatcaatc tgattttaat atcgccaaaa tagctgcatt taatggacgc
     1561 aataatccgc atacttattt ttggggagaa atagcacgcc agttacaaaa agaaggcttg
     1621 tttagagagt attgggagtc tggtgcaaaa gctcctgatg agcaagcatg gttacgttta
     1681 tttgaaggtg atgagcctgt tttgatttta ttggacgaaa tgccgcctta ttttcattac
     1741 tacagcactc aagtattagg gcaaggtacc attgctgatg tgattacccg cgctttttct
     1801 aatatgctca ccgcagcaca aaaaaagaaa aatgtttgta ttgttgtttc tgacttagag
     1861 gcagcctacg acacaggtgg aaagctaatt caacgtgctt tagatgatgc aactcaagag
     1921 ctaggtcgag ctgaggtatc catcacacct gttaatttag aatcaaatga aatttatgaa
     1981 attcttcgca aacgcttgtt tgttgccttg ccacaaaaaa gtgaaattgc agagattgca
     2041 tctgtatatg gagcaagact tgcagaggcc gcaaaagcaa aaacggttga gcgaagtgcg
     2101 gaggccttag caaatgatat agagtcaaca tacccattcc atccaagctt taagagtatc
     2161 gttgcgctat ttaaagaaaa tgaaaaattt aaacaaacgc gtggcttaat ggagttggtt
     2221 tctagattgc ttaagtcagt ttgggaaagt aaagaagatg tatatttaat tggcgcacag
     2281 cattttgata tgtcaatttc ggaggttaga gagaagttag ccgaaatttc agaaatgcgt
     2341 gatgtgattg caagagatat ttgggactca actaatagtg cgcatgcgca gcttattgat
     2401 ataaataacc acaataatca tgctaagcaa gtgggaacat tgttattaac agccagctta
     2461 tctacggcta ttaactcagt aaaagggctt tccgaaagtg aaatgctcca atgtttaatt
     2521 gacccaaata atcaagccag cagttataga acagctcttg cagagctgca aaaatcagca
     2581 tggtatttac atcaaacaca agaagggcga cattatttta gtcacctaga aaatctaacc
     2641 aaaaagctgc aaggctatgc agaaaaagca ccacaaaaca aaattgatga gcttgttcgt
     2701 cagcgtcttg aagaaatgta taagccaaca accaaagaag cctatgatag agtgttgcca
     2761 ttaccagaaa tggatgaggc agaagccgct ttaaaaacag gccgagtttt attaattatc
     2821 agtcctgatg gaaaaacacc acctggtgtt gtggcaaaat tttttagaga gctaatcaat
     2881 aaaaataata tactggtttt aacaggcgac aaatcgtcta ttgccagtat cgaaaaagcc
     2941 gctagacatg tgtacgcggt tcgtaaagcc aatgaactcc ctgtatcaca tcttcagcgt
     3001 aaagagcttg atgaaaaaaa agcccagtac caacaagact ttcaaacaac ggtactttct
     3061 gtttttgata aattgctcat tcctagcttt aaaggtgaag atgtattacg ggcaaaagtg
     3121 cttgataata catacccctc aaaccagcct tataatggcg aacaacaaat tattaaaacc
     3181 ttaaccgctg accccatcaa actgtattta caaattacag aaaactttga tgcgctgcgt
     3241 acaagagcgg aaagtttatt atttggctct caggatgaag cacgtaaaac cgacttatta
     3301 gacaaaatga agcaaaaaac acaaatgccg tggcttcaca ataaaggttt tgatgccttg
     3361 attagagagg cagcacaacg cggtgtttgg gaagatatgg gtaatggcta tattaccaaa
     3421 aagccaaagc caaaaatgac cgatgtgatg attagtgaag ataacttgcc cgatgatttt
     3481 ggcacagtac aattaaaaat agatgtgacc aatgcaggca atagtcctag aattcatttt
     3541 gccgaagatg gggtagtgtc cgaaagtagt cctatcttaa aagaaaatgt actctcgaca
     3601 aaagcgttaa gggttcagtt tttggccgtt gacccgactg gcaaaaatca aacaggaacg
     3661 cctaaaacgt ggacaaataa actcactatt cgtaataagt ttaatgagca atcaagaact
     3721 gttgaattat ttgttgcccc taaaggcaac attaaatata cccttgatgg ctctgaggct
     3781 agaaatggta ttgaatacac acaagaaatt aaattaggca atcaagaggc caacatttat
     3841 gtgtttgccg aatgtgatgg aatagaagaa aagcgcaaat ttagttttcc taaagctggc
     3901 tctacagaag tgccaatcat taaagagaaa ccagcggtat tggtcagcca accacccaaa
     3961 cgtttagata gttctgccaa aacctatgac ggcttaaaac ttgccaaaga aaaaaacatt
     4021 gagtttgagc atgttatgct tacggctggc tcttcgccaa aagtcattac tttgtcacta
     4081 ggtgaaatac gcatcaatgc aacatttatt gaggccgcat tagcccattt gcaaaccttg
     4141 ttgccaccag atgcgccgtt agtgatgagt tttaaaaagg tttataccca tacagggcat
     4201 gacttagagc aatttgccaa acaactgagc attgaaatca aaaatggtga ggtggagcaa
     4261 aaatgagcaa taccatagac tttggcgcgt ctagcgaatt tggtatgcac catttttatg
     4321 tagaaatccc tgttgcgcca cgcgatgcga tttgtattta tgaagacttt ggctttgatg
     4381 gtaatgatgc caaacgtgaa accgtagagt gtcgattgat gttggctcgt gagttgtgga
     4441 gcaaaattag agatgatgcc agacgtgatt ttaatgcgcg tttaaaagcc aaaaaacaaa
     4501 gcacaggcag ttggacaaca ggccgcgttc gtttagaccg atttttaggc cgagagttat
     4561 gcgtattagc ttgggctgcc gagcatgcct cgcctgatga gtgtttggtg atttgccaaa
     4621 agtggttggc actaagacca gaagagcgat ggtggttgta tagcaaaacg gctgccgaag
     4681 caggattagc caatcaaaca caacgaggtt ggcgcaaggc attgtattgt gcattgtctg
     4741 atggccaaaa cattaaagcc acgcaaaagg ttacgccaaa aagcaaaaaa aaagatgatg
     4801 aaggtttaga aacatttaac cttaatcttt ttgatgtgta aaacagagga aacagtcatg
     4861 caagcttatt acgaaatcgc aacctacatt cccagtaatc accagttaca t
//
LOCUS       DAOQMB010000149         4889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8061_length_4889_cov_14.411667, whole genome shotgun sequence.
ACCESSION   DAOQMB010000149 DAOQMB010000000
VERSION     DAOQMB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8061_length_4889_cov_14.411667"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PLY05_06315"
     CDS             <1..576
                     /locus_tag="PLY05_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNC02954.1"
                     /translation="SQLAQTGQALYTGTCRHQLHYQPACAARVLVPSSTYIQFEDRVF
                     GNQYEYLSQTVGDFVIYRRDGLFAYQLAVVVDDALQGITHIVRGADLLDNTSRQIYLQ
                     QQLGYPRINYAHLPLIVNHLGEKLSKQTGATALDLTTNPSPVLWQLLGLLGQHPPSCL
                     QFSAKDELLMWAIAHWQFANIPKQLKLEHIF"
     gene            595..771
                     /locus_tag="PLY05_06320"
     CDS             595..771
                     /locus_tag="PLY05_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02955.1"
                     /translation="MYIDRLVLTFIAAAYLLSPAIIEWWSLGGTEWTRPFMIWLGIIL
                     VSFWIAKSRDIHDL"
     gene            761..>1965
                     /locus_tag="PLY05_06325"
     CDS             761..>1965
                     /locus_tag="PLY05_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HNC02956.1"
                     /translation="MTFNAVQLFSLGFIYLFLLFLIAHFSETGLISTKWIRNPIIYVL
                     SLGVYISAWGVYGVVGFAYETGYNYLAFYLGVSGAFLLAPVLLSPILRLSKNHQLSSL
                     ADLFAFRYRSQFVGSLTTIFMLLALLPLLALQIRAVSDTANILTQENHHHSIAIVFCL
                     AITLFSLLFGAKPLTPQYKHDGLVVTVAVESIIKLIMMLAVAGYALVAIFGGSDGLQD
                     WLSHNKDALELLYIPLKENGIWHSLILAFLFSAVVMPYMYHMVFTENFQPRSLLTASW
                     GLPLYLWLMAICVPIILWAGIKLELGINPEYFTIAIAQASQSSLLSLLVFIGGISAAS
                     GTIVVTLLALSSMVLNHLVLPLAQPSPDYNLYHWLVWVRRLLIVLIMGFSYAFYALFT
                     HQHTMTEMA"
     assembly_gap    1966..2065
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2066..3070
                     /locus_tag="PLY05_06330"
     CDS             <2066..3070
                     /locus_tag="PLY05_06330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC02957.1"
                     /translation="SWNRTLMEMTGISDDEVIGSRLTDLPEPWQTLLNDFLHSPNVQL
                     YNQEVVSQAQSRWLNLHRAFIGENEKAGELSSSQVIVLEDVTELERLESQLAHNERLA
                     ALGRLAAGVAHEIGNPVTGIACLAQNLKAEYKDPEIVEASTLILTQTQRITNIVRSLV
                     GFARTDAHMTPNTYSAVNLHDIIDEAINLLKLSGGKDYQFDNFCTDNILVRGNGQKLM
                     QVFVNLLSNARDASPPQSHIIVEAHSNGNYIHIDVEDFGHGLPEGTIRSTLFEPFVTT
                     KETGKGTGLGLALVHGIIGEHGGKIQLMDKADYDQGRGVIVQITLPAWLNDSNENDSQ
                     "
     gene            3067..4476
                     /locus_tag="PLY05_06335"
     CDS             3067..4476
                     /locus_tag="PLY05_06335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNC02958.1"
                     /translation="MKHILIVEDEMLIRSSLRRLLQRHHYQVSEAGSVHEATGNYRLD
                     EFDLIITDLRLPGELGTSLIELAKPTPVLIMTSFGTMKSAVDAMKQGAVDYISKPFDH
                     DEMVLSVERILREQQLKVENTALKQQQKQSTINGIIGSCPAMQELFKRITRVAPMGST
                     VLVRGESGTGKELVAKALHQESPRKDHALISVNCAAIPESLIEAELFGYEKGAFTGAN
                     TQRKGLVEAADGGTLFLDEIGELPLEAQARLLRLLQEKEVRRLGATQTRSVDVRLVAA
                     THRNLKQLAAEGRFREDLYYRLNVVELIIPPLRERGSDIIEIAQVLLEKTCQRLHVEP
                     VSFDDDSLSAMLAYRWPGNVRELENAIERAVILAEGNTISPDLLAIELDLPILTKPIE
                     PTDTLIDTSPKALVKETSLSHKLNDSEFQEQVLRYQDQMSETELAKRLGISRKSLWER
                     RQRLGIPRRRSTHIGDDTE"
     gene            4486..>4889
                     /locus_tag="PLY05_06340"
     CDS             4486..>4889
                     /locus_tag="PLY05_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNC02959.1"
                     /translation="MLQYHSARDYGINPEDLGTPALQVINELHKAGFQAFVVGGGIRD
                     LLLGLHPKDFDIATNATPEQVKRVFGRQCQIIGRRFQLAHVYQERHLFEVATFRAPHD
                     NQSQRSQTSAHGMITRDNVWGTIDQDALRRDFT"
BASE COUNT         1342 a          974 c          995 g         1478 t
ORIGIN      
        1 agtcaattag cccaaactgg ccaagctctt tatacaggta cttgtcgtca tcaattacac
       61 tatcagcctg cttgtgccgc acgtgtatta gttccctcat caacctatat tcaatttgaa
      121 gatcgtgttt ttggcaatca atacgaatac ctgagtcaaa cagttggtga ttttgtcatt
      181 tatcgccgtg atggtttatt tgcctatcaa ctcgcggtag tggttgatga tgcactacaa
      241 ggcattactc atattgttcg tggtgcagat ttactcgata acaccagtcg acaaatttat
      301 ttgcaacaac aactaggcta tccacgcatt aattatgcac atctccccct gattgtgaat
      361 catttaggcg aaaaactgtc caaacaaaca ggagcaaccg cactcgattt aacgactaac
      421 cctagccctg tgttatggca acttttaggc ttattaggcc aacatccccc ctcctgctta
      481 caattctcag caaaagatga attattaatg tgggcaatcg cacattggca attcgcaaac
      541 attcccaaac agcttaaact agaacatata ttctaaaatt tttagggaat atccatgtat
      601 atcgatcgat tggtcttaac ctttattgct gctgcatatt tgttatcacc tgccattatt
      661 gaatggtgga gcttgggcgg cacagaatgg acacgccctt ttatgatttg gctaggcatt
      721 attttagtta gcttttggat tgcaaaaagt agagatattc atgaccttta atgcagtaca
      781 gttatttagc ttaggcttta tttatctatt tttattattt ttaattgccc actttagtga
      841 aacgggatta atctctacta aatggattag aaacccgatt atttatgttt tatcactggg
      901 tgtttatatc agtgcatggg gtgtttatgg cgttgtcggg tttgcctacg aaacaggtta
      961 taactattta gcgttttatt taggggtatc aggtgcgttt ttacttgcgc ccgttttact
     1021 tagccctatt cttcgcttat cgaaaaacca tcaacttagc tcgttggccg atttatttgc
     1081 tttcaggtat cgcagtcaat ttgtgggttc tttgactacc atttttatgc tcttagcctt
     1141 attaccgcta ttagcattac aaattcgagc agtgagtgat acggctaata ttttaacgca
     1201 agaaaatcat catcacagca ttgcgattgt tttttgtttg gcaattacct tatttagctt
     1261 attgtttggt gcaaaacccc tcacccctca atataaacat gatggtttag tcgtcaccgt
     1321 tgccgttgaa tcgattatta aattaataat gatgttagcg gttgcaggct atgcacttgt
     1381 cgcaattttt ggtggcagtg atggtttgca agattggctc tctcacaata aagatgcttt
     1441 agaactgtta tatattcccc tcaaagaaaa cggtatttgg catagcttaa ttctggcatt
     1501 tttgttttcg gctgtggtga tgccctatat gtatcacatg gtatttaccg aaaattttca
     1561 accacgctca ttattaaccg ctagttgggg tttgcctctg tatttatggt taatggcgat
     1621 ttgtgtaccg attattttat gggcaggtat taaactcgaa cttggcataa atcctgagta
     1681 ttttaccatt gccattgctc aagccagtca aagcagtctg ttgtcgttat tggtgtttat
     1741 tggaggtatt tcggcagcca gtggcacaat tgtcgtcacc ttattagctt tgtcttctat
     1801 ggtactcaac catttagtct tgccattggc acaaccctcg cctgattata atttgtatca
     1861 ttggttagtt tgggtcagac gtttattgat tgtgttgatt atgggctttt cttacgcttt
     1921 ttatgctcta ttcactcatc aacataccat gaccgaaatg gccatnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnagttg gaatcgtacc ttaatggaaa tgacgggtat
     2101 ttctgatgat gaagtgattg gctcacgact gaccgactta cccgaaccgt ggcaaacctt
     2161 actcaatgac tttttacata gccctaatgt gcagctatat aatcaagaag tggtcagcca
     2221 agcacaaagt cgttggttaa acctacatcg tgcctttatt ggtgaaaacg aaaaagcagg
     2281 tgaattaagt agcagccaag tgattgtttt ggaggatgtg accgaactgg aacgtctcga
     2341 atcacaactg gcacataatg aacgcttagc tgctttagga cgcttggcgg caggtgttgc
     2401 ccacgaaatt ggtaatcctg tcactggtat tgcttgtttg gcacaaaatc ttaaagccga
     2461 atataaagac cctgaaattg tcgaagccag tacactaatt ttgactcaaa cccaacgtat
     2521 cactaatatt gtccgctctt tagtcggttt tgcgcgcact gatgcccaca tgacacctaa
     2581 tacctatagt gcggtaaatt tgcatgacat cattgacgaa gcgattaatt tactcaaatt
     2641 atcaggcgga aaagactatc aatttgataa tttttgtaca gataatattt tggtgcgtgg
     2701 taatggtcaa aaactgatgc aagtatttgt taacttatta agtaatgcgc gtgatgctag
     2761 cccaccacaa agccatatta ttgttgaagc ccatagcaac ggtaactata ttcatattga
     2821 tgttgaagac tttggtcatg gtttacctga aggcacgatt cgctccacct tatttgaacc
     2881 ttttgtcacc accaaagaaa caggtaaagg aacaggttta ggcttagccc ttgtacatgg
     2941 tatcattggc gaacatggtg gcaaaattca gctcatggat aaagctgatt acgaccaagg
     3001 acgtggtgtg attgtccaaa ttaccttgcc tgcatggctt aatgacagta acgagaacga
     3061 tagtcaatga agcatatttt gattgttgaa gatgaaatgt tgattcgctc tagcctaaga
     3121 cggttattgc aacgacatca ttatcaagtc agtgaagctg gctcggtaca cgaagcgaca
     3181 ggcaactatc gtttagatga gtttgactta attattaccg atttgcgttt acccggtgaa
     3241 ttgggtacaa gtttaataga attggccaaa cctacacctg tgctaattat gaccagtttt
     3301 ggcacgatga aatcggcggt tgatgccatg aaacaaggcg cggttgacta tatttctaaa
     3361 ccctttgacc acgatgaaat ggtattaagt gttgaacgta ttttgcgcga acaacaactt
     3421 aaagtggaaa acacagctct caaacaacaa caaaaacaaa gcacgattaa cggcattatt
     3481 ggtagctgtc ctgcgatgca ggaattgttt aaacgtatta ctcgtgttgc acccatggga
     3541 tcaacggttt tagtgcgtgg cgaatcaggt actggcaaag agttagttgc taaagcctta
     3601 catcaagaaa gcccacgtaa agatcatgcc ttaatttcgg ttaactgtgc ggctattccc
     3661 gaatccttaa ttgaagccga attatttggc tacgaaaaag gcgcgtttac aggggcgaat
     3721 acacaacgta aaggtttagt cgaagccgcc gatggcggca cattattctt agacgaaatt
     3781 ggcgaattac cgctcgaagc ccaagcacga ttattacggt tattacaaga aaaagaagtg
     3841 cggcgtttag gagcaacaca aacccgctcc gttgacgtgc gtttagtcgc ggctactcat
     3901 cgaaatttaa aacaattagc ggctgaaggt cgttttaggg aagacttata ttatcgctta
     3961 aatgtcgtag aattaatcat tcctccctta cgcgaacgtg gcagtgacat catcgaaatt
     4021 gctcaggttt tattagaaaa aacctgtcaa cgtttgcatg ttgagcctgt ctcttttgat
     4081 gatgatagtt tgtcggcaat gttagcttat cgttggccgg gtaatgtgcg agagctagaa
     4141 aacgccattg aacgtgcggt cattttggcc gaagggaata ccattagtcc cgatttatta
     4201 gccattgaat tagatttacc cattttaacc aaacctattg agccaacaga cacactcata
     4261 gacacatcgc ctaaagcact tgtcaaagaa acttcgctta gccataagct caatgacagt
     4321 gaatttcaag agcaggtgtt acgttatcaa gaccaaatgt ccgaaactga actcgctaaa
     4381 cgtttaggca ttagtcgtaa atctttatgg gagcgtcgtc aacgcttagg cattccacgt
     4441 cgtcgtagta cacatattgg cgacgataca gagtaaacat catttatgct gcaatatcat
     4501 tctgcccgtg attatggaat caatccagaa gacctcggca cgcctgccct acaagtgatt
     4561 aatgaactac acaaagcagg ctttcaagcc tttgtcgttg gtggaggcat acgcgatctg
     4621 ttattaggtt tacaccctaa agattttgac atcgccacca atgccacgcc cgaacaagtt
     4681 aaacgtgttt ttggccgtca atgtcaaatt attggtcggc gttttcaatt agcgcatgtg
     4741 tatcaagagc gtcatttatt tgaagtcgcc acctttcgcg caccacatga caatcaaagt
     4801 caacgtagtc aaacctctgc acatggcatg attactagag ataatgtgtg gggaacgatt
     4861 gaccaagatg cgttacgccg tgactttac
//
LOCUS       DAOQMB010000150         4884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8084_length_4884_cov_12.249741, whole genome shotgun sequence.
ACCESSION   DAOQMB010000150 DAOQMB010000000
VERSION     DAOQMB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8084_length_4884_cov_12.249741"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..388
                     /locus_tag="PLY05_06345"
     CDS             194..388
                     /locus_tag="PLY05_06345"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC02960.1"
                     /translation="MRTNIVIDDALMHDALKATGLKTKKEAVELGLKMLIKLKQQESI
                     KAFRGKLKWEDDLEDMRLNP"
     gene            385..786
                     /locus_tag="PLY05_06350"
     CDS             385..786
                     /locus_tag="PLY05_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNC02961.1"
                     /translation="MILIDSSVWIDYFNGQPTPEANKLDSLLGVEPLAIGDLILTEVL
                     RGFRVDTDYQTAKKLLLTLTVFDMLGTDLAIQAADNFRFLRKRGVTVRKTADVIIATH
                     CIEYGHSLLFADKDFLPFVTHLGLKAVTTNT"
     gene            823..3564
                     /locus_tag="PLY05_06355"
     CDS             823..3564
                     /locus_tag="PLY05_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNC02962.1"
                     /translation="MLSPNQIRANARKFSLEWKDETRERAESQTFWNEFFAVFGVNRR
                     KVGIFEATFKKLKGTQGFIDVYWPKKLVCEQKSRGADLAKATTQALDYLQSIAKVAEE
                     DLPRYVIVCDFEHLHLLDIETQQQQKILVANLADNIELFGFISGYETEFRQQQEAVNI
                     AAAERMGRLHDQLKENGYDGHELRVMLIRLLFCLFAEDTQIFNKNQFYNFLVQRTQQD
                     GSDLAAWISQLFDTLNREKRLKNLDEQLNAFAYINGELFKEVIPPAAFDAKMRQELID
                     ACETDWQAISPEIFGALFQSVMNKEERRNLGAHYTSEQNILKVINSLFLQELRDELAT
                     IKTHKQVNMRAAKLDVFHDKIARLTFLDPACGCGNFLVVAYRELRLLELEVIEQIQKD
                     STQLIMDVSALIRCNVNQFYGIEIEEFPSQIARVAMWLVDHQMNLLVSDRFGMHYARI
                     PLKRSAAILNANALQTDWPVTDYILGNPPFIGSKLIDKNQRIAFDSVMSVVPKSGVLD
                     FVSAWYVKAAQLIKANAHIRCALVSTNSITQGEQVGILWGWMLAQGMVIQFAHRTFVW
                     QSAAKGKAAVHCVIIGFGTDNTLKKTIFDYEDGKGLPMPISANNINAYLVDAKNIFIE
                     NKRESLIDMSPMNYGSFALDDGYYTLDVEEVEALIKTNSAIQPFIKPFIGGRELLSGK
                     KRYCLWFFDANIKTLRSIPELMARIDAVQKWRSESDRENTKKLAQTPMRFAEIRQPKS
                     QYIAIPTVSSERRNYLPIAFLSPDVIASNQLYLIDNANLYIFGIMTSIMHNAWMRTTC
                     GRLKSDYRYSASIVYNNFPWPLNPTDKQKQTIEAAAQAVLDARAAHPTCSLADLYDPL
                     TMPANLLKAHQQLDKAVDAAYGKTKFANEAERVAFLFELYQQYTASIEQVKPKKTRK"
     gene            complement(3630..4586)
                     /gene="lipA"
                     /locus_tag="PLY05_06360"
     CDS             complement(3630..4586)
                     /gene="lipA"
                     /locus_tag="PLY05_06360"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNC02963.1"
                     /translation="MSEIVKVVTGEKLRGAEKVSRIPVKVIPTETLPRKPDWIRVRIS
                     NPNEVQRIKGLLREQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAH
                     GRPNALDADEPRHLAETVANLKLKYVVVTSVDRDDLKDGGAQHFVDCISQTRSLNPNT
                     KIEVLVPDFRGRMDIALRIMRECPPDVFNHNIETVPRIYKAIRPGSDYQHSLTLLKRF
                     KEECPDVTTKCGLMVGIGETKEEVLAVLDDLRAHNVDLVTIGQYLQPSKNHAPVDRFV
                     HPDEFKEYADYGKKLGFTNIWSGPMVRSSYHADRQYEGKALG"
BASE COUNT         1490 a          907 c         1023 g         1464 t
ORIGIN      
        1 atattaaacc cgaagcacgc gaacaaattt tggtggatta taaaaattat ttagccaatt
       61 tagacagttt aacaccgttg cgttatccaa aaatggatga gtttgatagc aagttgtatc
      121 cgaagtaatc gtagctaaaa ttatctataa tgtgtatatt aaaatataaa atacacatta
      181 tagagttcaa aaaatgcgta ccaatattgt tattgatgat gctttaatgc acgatgcttt
      241 aaaagccaca ggcttaaaaa ccaaaaaaga agcggttgag ttggggttaa aaatgctcat
      301 taaactcaag caacaagaat cgattaaggc ttttcgtgga aaattgaaat gggaagatga
      361 ccttgaagac atgagactca atccgtgata ctgatagact caagtgtttg gattgactat
      421 tttaatggcc aacccacgcc agaggccaat aaattagata gcttgcttgg tgttgaaccg
      481 cttgctattg gcgatttgat tttaacggag gtattacgcg gatttcgtgt agatacagat
      541 tatcaaactg cgaaaaaatt attgctcaca ttaactgtgt ttgatatgtt agggacagac
      601 ttagccatac aagccgccga taactttcgt tttttacgca agcgtggcgt taccgttcgt
      661 aaaacagcag atgtgattat tgccacacat tgtattgaat atggccattc tctgttattt
      721 gccgataaag actttttacc tttcgtcacc catttaggct taaaagcggt gacaacaaat
      781 acctaaacct gttttttaca cctcaataca aggagcgtta ttttgttatc ccccaatcaa
      841 attcgtgcca atgcgcgtaa atttagtctt gaatggaaag acgaaacacg cgaacgagcc
      901 gaatctcaaa ccttttggaa cgagtttttt gcagtatttg gcgttaatcg ccgtaaagtg
      961 ggtatttttg aagccacctt taaaaagctc aaaggcacac aaggctttat tgatgtgtat
     1021 tggccaaaaa aattggtctg tgagcaaaaa agtcgcggtg cagatttagc caaagccacc
     1081 acgcaagctc ttgattattt gcagtcaatt gccaaagttg ccgaagaaga tttgccgcgt
     1141 tatgtgattg tctgcgattt tgagcatttg catttattgg atatagaaac acaacaacag
     1201 caaaaaattt tagtggctaa cttagccgat aacattgagt tatttggttt tatttcgggc
     1261 tacgaaactg agtttagaca acagcaagaa gcggtcaaca ttgctgccgc cgagcgcatg
     1321 ggacgtttgc acgaccaact caaagaaaac ggttatgacg gccacgaatt gcgtgtgatg
     1381 ttgattcgct tgctattttg tttatttgcc gaagacacgc aaatttttaa taaaaatcaa
     1441 ttttataatt ttttagtgca acgtactcaa caagatggct cagatttagc ggcatggatt
     1501 agccaattat ttgatacgct aaaccgcgaa aaacgcctta aaaatttaga tgagcaatta
     1561 aacgcctttg cgtacattaa cggtgagctt tttaaagaag ttattccgcc tgcggctttt
     1621 gatgccaaaa tgcgtcaaga gttaatagat gcctgcgaaa cagactggca agcgatttct
     1681 cccgaaattt ttggcgcatt gtttcaatcg gtaatgaata aagaggaacg gcgtaactta
     1741 ggggcgcatt acaccagcga gcaaaacatt ttaaaagtga ttaactcgtt atttttacaa
     1801 gaattaagag acgaattggc aactattaaa acgcataaac aagtgaatat gcgtgccgct
     1861 aagttagatg tttttcacga taaaatagct cgattaacgt ttttagaccc tgcctgtggt
     1921 tgtggtaatt ttttggtggt ggcttacaga gaattgcgtt tattagagtt agaggtgatt
     1981 gagcaaattc aaaaagactc aacgcagtta atcatggacg tttcggcttt gattcgttgt
     2041 aatgttaatc aattttatgg cattgagatt gaggagtttc cgtcacagat tgcgcgggtg
     2101 gcgatgtggt tggttgacca tcaaatgaat ttgttggtgt ctgaccgctt tgggatgcac
     2161 tatgcgcgta ttccgctcaa gcgaagtgcg gctattttaa atgcgaatgc cttacaaacc
     2221 gattggcctg ttaccgatta tatattgggt aatccgccgt ttattggttc aaagttaata
     2281 gataaaaatc aacgtattgc ttttgatagc gtgatgtcgg ttgtacctaa atcgggtgta
     2341 ttagattttg tgtcggcatg gtacgttaaa gccgcgcagc ttattaaggc aaatgctcat
     2401 atacgttgtg ccttggtttc gaccaattcg attacgcaag gcgagcaagt gggtattttg
     2461 tggggttgga tgttggcgca agggatggtt attcaatttg cacaccgtac ttttgtttgg
     2521 caaagtgcgg ctaaaggcaa agcggcggtg cattgtgtga ttattggttt tggtacagat
     2581 aatacgctta aaaagacgat ttttgattat gaagatggca aaggtttacc tatgccaatc
     2641 agtgccaata atatcaatgc gtatttggta gatgctaaaa atatctttat tgaaaataaa
     2701 agagaatctc ttattgatat gagtccaatg aattacggaa gttttgcttt agatgatggc
     2761 tattacacct tagatgtaga agaggttgag gcactgatta aaacaaatag tgcgattcag
     2821 ccttttatta aaccatttat aggtggtaga gagttgttaa gtggtaaaaa aagatactgc
     2881 ctttggtttt ttgatgcaaa tataaaaacg ttaaggagta ttcctgaatt aatggcgcga
     2941 attgacgcgg ttcaaaaatg gcgttctgaa agcgatagag aaaatacaaa aaaattagca
     3001 caaaccccga tgcgatttgc tgaaattaga caacccaaaa gtcagtatat tgccatacct
     3061 acagtttctt cagaacgaag aaattattta cctattgcat ttttaagccc tgatgttatt
     3121 gcatctaatc aattatatct tattgataat gctaatcttt atatattcgg catcatgact
     3181 tccatcatgc acaacgcatg gatgagaaca acgtgcggac gtttaaaaag tgattatcgc
     3241 tattccgctt ccattgttta caacaacttt ccgtggccat taaacccaac cgacaaacaa
     3301 aaacaaacta tcgaagccgc agcacaagcg gtattagatg cacgggcagc acatccaaca
     3361 tgttccttag ccgatttata cgacccttta accatgcctg ctaacttgct taaagcccat
     3421 caacaactcg acaaagccgt agatgccgcc tatggcaaaa ctaaatttgc taatgaggcg
     3481 gagagggtag cgtttttatt tgagttgtat caacaatata ccgcgtctat tgagcaagtt
     3541 aagcccaaaa aaacgagaaa atagtattcc cttcgactcc gctcagggaa cagctttaat
     3601 ttttgttgat tgagcggagt cgaagccaat caccccaacg ccttcccctc atactgccta
     3661 tccgcatgat acgaagacct taccattggc ccactccaaa tattggtgaa gcctaatttt
     3721 ttgccataat cggcatattc tttaaattca tcaggatgga caaaacgatc aacaggtgcg
     3781 tgattttttg acggctgtaa atattggcca atggttacga ggtcaacatt gtgggcgcgt
     3841 aaatcgtcca atacggctaa aacttcttct ttggtttcgc caatcccgac catcaaacca
     3901 catttagtgg ttacgtctgg acattcttct ttaaagcgtt taagcaacgt gagagagtgt
     3961 tgataatccg aaccaggtct aatcgcttta taaattctgg gcacggtttc gatgttgtgg
     4021 ttaaacacat caggtggaca ttcacgcata atccgcaagg caatatccat acgacctcta
     4081 aaatcaggta ctaaaacttc aattttagta tttggattca acgagcgtgt ttggctaata
     4141 caatcgacaa aatgttgtgc accaccatct tttaaatcgt cacggtcaac cgaagtcact
     4201 acgacatatt ttaacttgag attagccacg gtttcggcta aatgacgtgg ttcatcagca
     4261 tctaaagcat taggacggcc atgtgcgaca tcgcaaaatg gacaacggcg tgtacaaata
     4321 tcacccataa tcataaaagt agctgtgcca ccgccaaagc attcaggcag attaggacaa
     4381 gcggcttctt cacaaacagt atgtaatttc tgttcgcgta ataagccttt aatacgttgg
     4441 acttcgttgg ggttagaaat acgaacacga atccagtcag gtttacgcgg tagggtttcg
     4501 gttggaatca ctttaactgg aatacgggaa actttttcgg caccacgtaa cttttcgcca
     4561 gtcacgactt taacgatttc gctcattagc aattgctctt gctgaaagga gatgaagaga
     4621 gggatgaagc tattttagcg taattgctac ttttacgcat ctttgattag gatttgctgt
     4681 tcattggcta gtttttggag attaacgaca attctttgac tttggatgcc tagttagagt
     4741 ttgcctatat agactgtttt taaaatcaag tattctcacg ctttaactca acctcccaaa
     4801 aaactcctca aattttacat attccatcaa gttagatgga gtaatagttt aggtattgat
     4861 tagatgaatg ctcgttttaa cagt
//
LOCUS       DAOQMB010000151         4863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8144_length_4863_cov_18.188020, whole genome shotgun sequence.
ACCESSION   DAOQMB010000151 DAOQMB010000000
VERSION     DAOQMB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8144_length_4863_cov_18.188020"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2046
                     /gene="recG"
                     /locus_tag="PLY05_06365"
     CDS             <1..2046
                     /gene="recG"
                     /locus_tag="PLY05_06365"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNC02964.1"
                     /translation="SVQQLKGVGTMLADKLAKLGLYTLQDVLFHLPRDYEDRSHITAI
                     DVVRSGASVLLEGEVLSCDVQNGKRQSLAVRLSDGSGQITLRFYHFYPQQKEHFKRNT
                     RLRVFGEVRLGASGLEMYHPEYKAVRVNEPLPDAKLTALYPTTEGLTQVRLRQLVSDA
                     LVFATPQSLPELLPPFINQRFDLLQALHTVHNPPNHIKRELLLLRVHPAQQRLAFEEL
                     TAHQISLAQRRQHVQILKAPVLAADCPLATQFLSLLPFTITNAQRRVWQEIAQDLQQA
                     KAMLRLVQGDVGAGKTVVAALAACHAIAQGWQVALMAPTEILAEQHFINFNQWFSQLG
                     FKVAWLSGKQGTKERNASLAVVASGEAHIVVGTHALFQDSVQFGRLGLVIIDEQHRFG
                     VEQRLALRQKGEQWGMCPHQLIMTATPIPRTLAMSAYGDLDTSVIDELPPNRTPVTTV
                     ALPNARRADVIERVRANCQTGKQAYWVCTLIEESEQLEAQAAQAIYEELQQELPELRL
                     GLVHGKLKASEKQAIMAEFKQGNLDLLVATTVIEVGVDVPNSSLMIIENAERLGLAQL
                     HQLRGRVGRGSTVSYCVLMYQPPLSHMGQERLAIMRATTDGFVIAEKDLELRGPGEVL
                     GTKQTGLVEFKVADLARDQQLLKTAQQLARNLLKDNPHNAQALVQRWLPNAPKYVAV"
     gene            complement(2142..>2766)
                     /locus_tag="PLY05_06370"
     CDS             complement(2142..>2766)
                     /locus_tag="PLY05_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNC02965.1"
                     /translation="MFKSLAADALGLSDIGKIIPPAEFDKTDVDDYVFHEDNEEIFVV
                     IKSKMDEYCFTNLAFIHLNGNLAISKKRMLNRYLYKHYSISDVRMETAGTVDLDVELK
                     FKVGGAEFSIDIDKKQITQIKAIYKALFAISERCKEIDRDMHVLQRTHDAVNAMFVLR
                     ELPENVVLNLPDIINQTVIQVEAGYHDRRKAIQQYDFAGVFKKFLPR"
     assembly_gap    2767..2866
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2867..4339
                     /locus_tag="PLY05_06375"
     CDS             <2867..4339
                     /locus_tag="PLY05_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008109641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance system multicopper oxidase"
                     /protein_id="HNC02966.1"
                     /translation="KTRQAVTVNGSTPAPTLYMKEGETVTIDVYNQLHEDSSIHWHGL
                     LVPSNMDGVPHLSFAGIKPQQTFRYQFPLIQSGTYWYHSHSGWQEQLGMYGALIIEPA
                     EGDPIKADREHVLILSDWTDDNPANILRFLKSESDFDNYQQPTFEELWHDIRQFGLKR
                     AFAKRQMWNQMRMSPTDFTDLSGVSTFTYLLNGSTSAANWTALFNRGETVRLRIINAS
                     AQSIFDFRIPELDMTVVGTDGGLVEPVTVDEIRIGVAETYDVLVKPQADAYTLFAQDI
                     ARSGFISGTLAVQQGLSAPIPAIDKAEWLTMTDMMGDMQGDEPAHHAHTEYSWTTDMR
                     VDYPRTNLDDAGINLRDNGRKVLTYADLYSFDELRDNEEEPTREIEMHLTGNMERYVW
                     GFDGVPYPEAKPVHIGVNERLRISLVNDTMMTHPMHLHGMWSDLRCPNGGLQVRKHTI
                     LVQPAQKVSFDVTGEQGRWAWHCHLAYHMESGMFREVVVD"
     gene            4448..>4863
                     /locus_tag="PLY05_06380"
     CDS             4448..>4863
                     /locus_tag="PLY05_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02967.1"
                     /translation="MKYLILLPISLVSSIAFSADNHQHDEHSQHQMTEISQPETHQHN
                     SQEANPSTSEHNHSSMNHDNMNHDMSSMQGGNAPADARHPDYSQGRVSSHTGTMHNHA
                     AWSVRINQLEQLNDDENTTVSEGVAWYGSDSQRLRLT"
BASE COUNT         1377 a         1001 c         1046 g         1339 t
ORIGIN      
        1 tccgtacaac agcttaaagg cgtgggcacg atgctcgccg ataagttagc caaattgggc
       61 ttatacacct tacaagatgt gctatttcat ttgccgcgtg attatgaaga tcgaagccat
      121 attactgcga ttgatgtggt cagaagcggt gcgagtgtgt tgttagaagg cgaagtgctg
      181 agttgtgatg tacaaaatgg caaacgtcaa tctttagccg tgcgtttgag tgatggttca
      241 gggcaaatta ccttacgctt ttaccacttt tatccccaac aaaaagaaca ttttaaacgt
      301 aatactcgtt tgcgggtgtt tggtgaagtg cgtttagggg ctagcggctt agaaatgtat
      361 catcctgaat ataaagcggt gcgcgttaat gaacctttac ctgatgccaa actcacggca
      421 ctctacccca ccactgaagg tttaacccaa gtgcgtttac ggcaactagt cagtgatgcc
      481 ttagtttttg ctacgcccca atcattgccc gaactactcc ccccctttat taatcaacgt
      541 tttgatttat tacaagcctt gcacacggta cataatccgc ccaatcacat taaacgtgaa
      601 cttttattgc tgcgtgttca tcctgctcaa caacgtttag cttttgaaga attaaccgcc
      661 catcaaatta gtttggcgca acgtcgtcag catgtgcaaa ttttaaaagc acctgtgtta
      721 gcggcagatt gcccgttagc tactcagttt ttaagtttat taccgtttac cattaccaat
      781 gctcaacggc gcgtgtggca agagattgcc caagacctac aacaagccaa agccatgtta
      841 cgtttggtgc aaggggatgt gggtgcagga aaaaccgtcg tggcggcatt ggcggcttgt
      901 catgcgattg ctcaaggttg gcaagtggcg ttaatggctc cgactgagat tttagccgaa
      961 caacatttta ttaattttaa tcagtggttt agccaattag gttttaaagt ggcttggttg
     1021 agtggcaaac aaggcactaa agaacgtaac gcctcgcttg ctgttgtggc gagtggcgag
     1081 gcacatattg ttgtgggtac acacgcctta tttcaagaca gtgtgcagtt tggtcgtttg
     1141 gggttagtaa ttattgatga gcaacatcgt tttggtgtcg aacaacgctt ggctttacgc
     1201 caaaaaggtg aacaatgggg catgtgtcca catcaattaa ttatgaccgc cacccctatt
     1261 ccgcgtactt tagcgatgag tgcttatggt gatttggata cctcagtcat tgatgagttg
     1321 ccgcctaatc gtacgcctgt cactacggtt gctttaccca atgcccgtcg tgctgatgtg
     1381 attgagcgtg ttcgagccaa ttgccaaaca ggtaaacaag cgtattgggt atgtacttta
     1441 attgaagagt cggaacaatt agaagcacaa gctgcccaag caatttatga agaattacaa
     1501 caagaattgc ccgaactgcg tttaggcttg gtgcatggca agttaaaggc cagcgaaaaa
     1561 caagcgatta tggctgagtt taaacagggt aatttggatt tgttagtggc aacaacagtg
     1621 atcgaagttg gtgttgatgt gccaaattcg tcattgatga ttattgaaaa tgccgagcgt
     1681 ttgggcttgg cgcaattgca tcaattacgt ggtcgggtgg gtcgtggtag tacggtgagt
     1741 tattgtgtgt tgatgtatca accaccgtta tcacacatgg gacaagaacg tttagcaatt
     1801 atgcgcgcca ccacggatgg ttttgtgatt gccgaaaaag atttggagtt gcgtgggcct
     1861 ggtgaagtat taggaactaa acaaacaggc ttggtagagt ttaaagtggc cgatttagct
     1921 cgcgaccaac agctattaaa aactgcgcaa caattggcgc gtaacttgtt aaaagataac
     1981 ccacataatg cccaagcctt agtacaacgt tggctgccca atgcaccaaa gtatgtggcg
     2041 gtttgatatt cattacgtca attaacaagc attgcatgaa ttgacgaaaa cccacgtttt
     2101 atacatcaaa aacgatgggt ttcgttcctc aacccatcta ctcaacgcgg caaaaacttc
     2161 ttaaacacgc cagcaaaatc atattgttga atggctttgc gtctatcgtg ataacctgcc
     2221 tcaacctgaa tcactgtttg attaataata tcaggcaagt tcaacaccac attttcgggc
     2281 aattcacgta acacaaacat cgcatttacc gcatcatgag tacgttgcaa aacgtgcata
     2341 tctctgtcta tttctttgca acgctcacta atggcaaata aggctttata aatggcttta
     2401 atttgagtga tttgtttttt atcaatatca atcgaaaact ctgccccacc gactttaaac
     2461 ttcaactcca catctaaatc gactgtcccc gccgtttcca ttcgcacatc actaatgctg
     2521 taatgtttgt ataaataacg atttaacatc cgttttttgc taatcgccaa attgccattt
     2581 aagtgaataa atgccaaatt ggtaaaacag tattcatcca ttttagactt gatgaccaca
     2641 aaaatttctt cattgtcttc atgaaaaaca taatcatcaa cgtcggtttt atcaaactca
     2701 gcagggggaa taatcttacc aatatcactc aaacccaaag catcggcagc taaactttta
     2761 aacattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaaa cacggcaagc
     2881 cgtgaccgtc aatggttcaa cacctgcacc aacgctgtat atgaaagaag gcgaaacggt
     2941 gactattgat gtttataatc aattacatga agacagctca attcattggc atggcttatt
     3001 agtaccatcg aatatggatg gtgtgccgca tttaagtttt gcaggcatca aaccgcaaca
     3061 aacatttcgt tatcaatttc cactcataca aagtggcact tattggtatc actctcattc
     3121 gggttggcaa gaacaattag gcatgtatgg tgcgctgatt attgaacctg ctgaaggaga
     3181 cccgattaaa gccgaccgcg aacatgtgtt aattttgtcc gattggacag acgataaccc
     3241 tgccaatatt ttgcgttttc tcaaaagtga aagtgacttt gataattacc aacaacccac
     3301 ctttgaagaa ttgtggcatg acattcgcca atttggctta aaacgcgcct ttgccaaacg
     3361 gcaaatgtgg aatcaaatgc gcatgagccc cactgatttt accgacttat caggcgttag
     3421 cacgtttacc tatttattaa atggctcaac cagtgctgct aattggacag cgttatttaa
     3481 tcgtggcgaa accgtgcgcc taagaattat taatgccagt gcacaaagta tttttgattt
     3541 tcgtattccc gaattagaca tgaccgtggt tggcacggat ggcggtttgg ttgaacctgt
     3601 gacggttgat gaaatccgta ttggcgtggc tgaaacttat gatgtcttag tcaaacccca
     3661 agctgatgcc tataccctgt ttgcccaaga tattgctcgt tcaggtttta ttagcggcac
     3721 attagcagtg caacaaggtt taagcgcacc cattcctgcg atagataaag ccgaatggtt
     3781 aaccatgacc gatatgatgg gtgatatgca aggcgatgaa cctgcacatc atgcccacac
     3841 tgaatatagc tggacaaccg atatgcgtgt tgattatccg cgcaccaatt tagatgatgc
     3901 aggtattaat ttgcgcgata acggtagaaa agttttaact tatgccgatt tatatagctt
     3961 tgatgagtta cgcgacaacg aagaagaacc gacacgcgaa attgaaatgc acttaacagg
     4021 caatatggag cgttatgtct ggggttttga tggtgtgcct tatcctgaag ccaaacctgt
     4081 gcatattggt gttaatgagc gactcagaat tagcttagtc aatgacacca tgatgaccca
     4141 tcctatgcat ttgcatggta tgtggagtga tttacgttgt ccgaatgggg gattacaagt
     4201 acgcaaacat acgattttgg ttcagccagc ccaaaaagta tcgtttgatg tcactggtga
     4261 acaaggtcga tgggcatggc attgtcattt agcctatcac atggagtcag gtatgtttag
     4321 agaagtggtt gtggattaaa ccctcaccct aaccctctcc caaagggaga gggaacttag
     4381 caatgaaagc tggggtgagg tttaaatgag aaaaaaagca ccacatctat cctatgagaa
     4441 caacataatg aagtatctaa ttttattacc catttcttta gttagctcaa tcgcatttag
     4501 tgccgacaat catcaacatg atgagcatag tcagcaccaa atgactgaaa taagtcagcc
     4561 tgaaacacat caacataaca gccaagaagc aaatcctagc acatctgagc ataatcatag
     4621 cagcatgaac catgataata tgaatcatga tatgagtagt atgcaaggtg gtaacgcccc
     4681 tgctgatgca cgtcatcctg attattctca aggtcgtgtt tctagtcata caggcactat
     4741 gcacaaccat gcagcatgga gtgtgcgtat taatcaactc gaacaactca atgacgatga
     4801 aaacaccacc gtcagcgaag gtgtggcgtg gtatggttct gatagtcaac gcttacgttt
     4861 aac
//
LOCUS       DAOQMB010000152         4861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8151_length_4861_cov_13.257595, whole genome shotgun sequence.
ACCESSION   DAOQMB010000152 DAOQMB010000000
VERSION     DAOQMB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8151_length_4861_cov_13.257595"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1365)
                     /locus_tag="PLY05_06385"
     CDS             complement(175..1365)
                     /locus_tag="PLY05_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016652695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HNC02968.1"
                     /translation="MKTLFKDFSFSAVIAGFVTVLVGFSSSGVLVFQAAQALGATPAQ
                     SSSWLWALCMGMGLTSIGLSWYYKTPIPTAWSTAAAAMLITIADHISLAEATGAFIVS
                     GLLITVAGFSGIFERLMKFVPMSLASAMLAGILLRFSLQAVNALQSHLAMILAMFIAY
                     LLCKRLKPRYAVLMALIVGIGTAYSQGLIHLQEISYQAVQPEWVKPSFSWHTVLGVAV
                     PLFLVTMTSQNMAGIAAIRSFSYHAPVSPLVGWTGLATVIFAPMGAFAISLAAITAAI
                     CLSSEAHEDKQRRYVAGIMSGIFYLLLGIFAATLANLLTALPKELVIGVAGIALLGTI
                     GNGLVQALAVEQEREAALICFVVTVSGINLLGIGAAFWGLIAGAFTLGLMGIVRVRGM
                     ANKN"
     gene            complement(1433..1645)
                     /locus_tag="PLY05_06390"
     CDS             complement(1433..1645)
                     /locus_tag="PLY05_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HNC02969.1"
                     /translation="MPYVNIKITKEGATAEQKAELIAGVTDLLVKVLNKNPATTFVVI
                     DEVDTDNWGVAGQSVTQRRIAERQNS"
     gene            complement(1743..2351)
                     /gene="gstA"
                     /locus_tag="PLY05_06395"
     CDS             complement(1743..2351)
                     /gene="gstA"
                     /locus_tag="PLY05_06395"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HNC02970.1"
                     /translation="MKLYYAAGACSLAPHIVSREADLNLDLIRVDTKSHLTADGVDFY
                     TINPKGSVPCLQLDNGQLLTEGPIICQYLADLANNTQLMPASGTLARYRVMEWQNYIT
                     SELHKSFSPLFNPNAGAETKALFAEQLKNKFTWLSSKLENTAYLTGDNFTAADAYLFV
                     VTNWSKFVGLDLSHLSALQAFMQRVATRPAVQMALKTEGLIK"
     gene            2569..3495
                     /locus_tag="PLY05_06400"
     CDS             2569..3495
                     /locus_tag="PLY05_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC02971.1"
                     /translation="MDRVEAMRVFCTVVEAGSFASAADKLTQSTSAVSRWVAQLESHL
                     QVRLLNRTTRRISLTESGQAYYERAVQWLHELENLESDVSTQAAMAQGLLKITTSLGF
                     ALSYLVPALDACQKVYPALKFQVSLSGNMVDLVEEGFDLALRVGSTGSQNVVAKQIGQ
                     TQLLYAAAPDYLKNYPPLNHPDDLLAHNCLIYQHHLSHNRLWTFLDKNQQQYDVTVQG
                     AISSDNSNFLAEAAAQGLGICCAPCFVLAPYLSNGQLVRILPDYHTPASHIYAVYPHR
                     KHLSAKVRLFIRFFEEWLAQQGLTDPTCGLGK"
     assembly_gap    3497..3596
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3750..4823
                     /locus_tag="PLY05_06405"
     CDS             3750..4823
                     /locus_tag="PLY05_06405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HNC02972.1"
                     /translation="MPIPTRAKILDFEKIGDPLADAVVDYLYQHKLRATNIWQLVQQQ
                     AQAGSKECQALCDEVFSVPEWVDWQQIERGAQVFLRSAPLALVAFALGALPLTYAVPN
                     IARVLASTKRLEQDAIRRLYETGSMVADVLAKGGLKADGVGLNSVLRVRLLHTFVRRH
                     VVIDDAKKGLNLPLAINQAEMAWVACGFSYVVLTGLERLGVYLTDDDKRAYHHLWRYA
                     NYLMGVNPALLADSPAQDALLYAQLKTVLCAPDDCGRLLTDTLAKSFAGQPPFFLPRR
                     ELGVLGSIVIPDKDLTQGLGFNLYPERHLLPLVKAVVYTASRTSTLATAQVADWGRDY
                     IRYVLDDGLKGRMADFQMQMGAH"
BASE COUNT         1416 a          979 c          938 g         1428 t
ORIGIN      
        1 tagtacatca caatattatt gctgctttat tcagtcatca tcatttagtg tgtttacaat
       61 cacaaatttt gaatatgcct agggtcgccg cgttgctgat aaaaattgac tcaggcaagg
      121 cacaaaacgc agcaaatagt tattctattg gcaagttttg tcacgcagaa tgagtcaatt
      181 tttattagcc atccccctca cacgaacaat acccatcagc cctaatgtga atgcccctgc
      241 aatcaatccc caaaaagctg cccctatgcc caataaatta atacctgata cagtgaccac
      301 aaaacaaatt aatgccgctt cgcgctcttg ttcaacagct aatgcttgaa ccaaaccatt
      361 accaatcgtg cctaataaag caatccctgc aaccccaatc actaactctt taggtaaagc
      421 tgtcaacaaa tttgctaaag ttgcggcaaa aatacctaat aacagataaa atatacccga
      481 cataatacct gccacataac gccgttgttt atcctcatgt gcctcactac tcaaacaaat
      541 tgctgcggta attgccgcta aactaatcgc aaatgcgccc attggtgcaa aaatcaccgt
      601 tgctaatcct gtccacccta ctaatggcga aacaggcgca tgataagaaa aactgcgaat
      661 tgccgcgatg ccagccatat tttgtgaagt catggtcact aaaaacaaag gcacagcaac
      721 ccctaacaca gtatgccaag agaagctcgg tttcacccac tctggttgca ctgcctgata
      781 gctgatttct tgcaaatgaa tcaagccttg actataagct gtgccaatac cgactattaa
      841 agccatcaat actgcataac gcggctttaa tcgcttacaa agtaaatatg cgataaacat
      901 tgccaaaatc attgccaaat ggctttgtag tgcgtttaca gcttgtaggc taaatctcaa
      961 taaaatacca gccaacattg ctgatgccaa agacatcggc acaaatttca ttaatcgctc
     1021 aaagatgccc gaaaaacctg caacggttat caataaaccc gaaacaataa atgcgcctgt
     1081 cgcttcggct aagctaatat ggtcagctat ggtaattaac atcgcggcgg cggctgttga
     1141 ccatgcagta ggaattggcg ttttgtaata ccaagataag ccaatactgg ttaaacccat
     1201 gcccatgcac aatgcccaca accaagagct actttgtgct ggtgttgctc ccaaggcttg
     1261 agcagcttga aacaccaaaa cgcctgagct actaaaaccg actaatacgg taacaaagcc
     1321 tgcaatcact gccgaaaaag aaaaatcttt aaataatgtt ttcataaaaa ttattaacaa
     1381 taaaaaatcc tcttagctac tttgattact aagaggattg atttcaaaaa aactaactat
     1441 tctgtcgttc agcaatacgc cgttgagtga cgctttgccc tgctactccc caattgtcag
     1501 tatcaacttc atcaataaca acaaaagtgg ttgcaggatt tttatttaat acttttacca
     1561 ataagtcagt gacaccagca atcagctcag ctttttgctc tgctgttgcc ccttctttgg
     1621 taattttaat attgacataa ggcataattt tttacttcct taagctgtcg tttgtgtact
     1681 cacatacctc cctgccttcg gcatcccccc ttattaaggg gggactgagg ggggtatttt
     1741 atttacttaa tcaaaccttc tgtttttaaa gccatttgta cggcaggacg ggtagccaca
     1801 cgctgcataa aggcttgtaa ggcacttaaa tgtgataaat ctaaaccaac aaatttagac
     1861 cagttggtga ctacaaacaa ataagcatct gccgccgtaa aattgtcacc tgttaaatac
     1921 gctgtatttt ctaatttact tgataaccaa gtaaatttgt tttttaactg ttcagcaaac
     1981 aatgctttgg tttctgcacc tgcatttggg ttaaacaaag gcgaaaatga tttgtgtaat
     2041 tcggaggtaa tataattttg ccactccatg actcgataac gagccaatgt gcctgatgct
     2101 ggcattaact gagtattgtt ggccaaatca gctaaatatt gacaaataat cggcccttca
     2161 gtgagtaatt gaccgttatc taactgtaaa catggcacac tacctttggg attaatagta
     2221 taaaaatcaa caccatctgc ggttaaatgg cttttagtat caacacgaat taaatctaaa
     2281 tttaggtctg cctcacggct aacaatgtgt ggagctaaag aacaagcacc tgctgcataa
     2341 taaagtttca ttattttttc ctcttatcag tggatcgttt gggtgatgat aataagactt
     2401 aacgcatttc atcgaaatta gcgcgttttg tgaacataaa accgttaaaa atggtttaaa
     2461 tgttcacaaa taagcgataa ccgcgtaagt cctaaataag ataactattt cttttagtgg
     2521 gataaagtga ttaaattaac ataatttatt taacaaagtt gaacaataat ggacagagtt
     2581 gaagcgatgc gcgtgttttg tacggtcgtt gaagcaggta gttttgccag tgctgccgac
     2641 aaactcactc aatccacctc tgccgtatct cgatgggtgg cacaattaga atctcatcta
     2701 caagtgcgtt tattaaatcg taccactcgg cgtattagct taaccgaaag tggtcaagcg
     2761 tattatgaac gtgcggtgca atggttacat gagcttgaaa acctagagtc tgatgtgagt
     2821 acccaagcag caatggcaca aggtttgtta aaaataacca cctctttagg ctttgcttta
     2881 agttatttag tgcctgcttt agatgcctgt caaaaagtat atcccgccct taaatttcag
     2941 gtgtcattat cgggcaatat ggttgatttg gtcgaagaag gttttgattt ggcgttaaga
     3001 gttggctcaa caggttcaca aaatgtggtg gcaaaacaaa taggccaaac tcaattgttg
     3061 tatgctgccg cacctgatta tctcaaaaat tacccacctt taaatcatcc tgatgattta
     3121 ttagcccata actgtttgat ttatcaacat catctcagcc acaatcgttt gtggactttt
     3181 ttggataaaa atcagcaaca atatgatgtc actgttcaag gagcaatctc tagtgataat
     3241 agcaactttt tggccgaagc tgcggcacaa ggtttaggca tttgttgcgc accttgtttt
     3301 gttttagcac cttatttaag caatggtcag ttagtgcgaa ttttgcctga ttatcacacc
     3361 cctgctagtc atatttatgc ggtttatcca catcgtaaac atttgtcggc taaggttcgc
     3421 ttatttattc gtttttttga agaatggtta gcacaacaag gtttaactga tccaacgtgt
     3481 ggtttaggca agtaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccaa
     3601 aaatccaacc aagcacttgc ttgttgaata tttattcagt atattggtac gaaagtgcca
     3661 catttaagct gattatttat tcgattgcgt gtttttgata aacataaaac cttaataaaa
     3721 gtatggtttt taataaagag gatatgattg tgcctattcc tactcgtgcc aaaattttag
     3781 actttgaaaa aattggtgac cctttagcag atgcagtggt tgattattta taccaacata
     3841 aactacgagc caccaatatt tggcaactgg tgcaacaaca agcccaagca ggcagcaaag
     3901 aatgccaagc gttgtgtgat gaagtgttta gcgtacctga gtgggtagat tggcaacaaa
     3961 ttgaacgtgg cgcacaggtg tttttgcgct ccgcaccatt ggctttagtc gcttttgcac
     4021 taggtgcatt gcccttaact tatgctgttc ctaacattgc ccgtgttttg gcctcaacca
     4081 aacgtttgga gcaagatgcg attagacgtt tatacgaaac aggttcaatg gtggctgatg
     4141 tgttggccaa aggtggccta aaagccgatg gcgtgggttt aaactcggtg ctaagagttc
     4201 gtttattaca tacttttgtg cgtcgtcatg tggtgattga tgatgctaaa aaaggcttaa
     4261 atttaccttt ggcaattaat caagccgaaa tggcgtgggt cgcttgtggc tttagttatg
     4321 tggtgctaac tggtttagag cgtttgggcg tgtatttaac cgatgatgat aaacgggctt
     4381 atcatcactt atggcgttat gccaattatt tgatgggggt caaccctgcc ttattggccg
     4441 atagccctgc tcaagatgct ttattgtatg cccaattaaa aacggtgtta tgtgcgcctg
     4501 atgattgtgg tcgtttatta acagatactt tagcaaaatc gtttgcgggt caacctccgt
     4561 tctttttgcc aagacgtgaa ttgggggtat tggggagtat tgtgattcct gataaagatt
     4621 taactcaagg cttaggcttt aatttgtatc cagaacggca tttattgcct ttagtaaaag
     4681 ctgtggttta taccgctagt cgcaccagta cccttgctac cgcgcaagtc gctgattggg
     4741 gacgtgatta tattcgttat gtgttagatg atggtcttaa aggtcgtatg gccgattttc
     4801 aaatgcaaat gggcgcacat taatattaaa ggcttgcaaa agctgagttt tggctcagct
     4861 t
//
LOCUS       DAOQMB010000153         4837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8216_length_4837_cov_16.161648, whole genome shotgun sequence.
ACCESSION   DAOQMB010000153 DAOQMB010000000
VERSION     DAOQMB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8216_length_4837_cov_16.161648"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4837)
                     /locus_tag="PLY05_06410"
     CDS             complement(<1..>4837)
                     /locus_tag="PLY05_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="strawberry notch C-terminal domain-containing
                     protein"
                     /protein_id="HNC02973.1"
                     /translation="SPEIRQQMDKEDDVYDFDFATLNVGQEEAADSIEKTQTTKKTLT
                     DLFYDHLKKGTLPENNAALRKMVAEFDGKEVDNYRLKEAQEMLEAAMARYSRDVVTSN
                     KNGDASTFNQLVDSYNKQPNLNIRTSTSVENQAYSTPAPLAFVAAKMANIGKSTKVYE
                     PTAGNGMLLITALPENATVNEMESIRFNNLKAQGFNAHQGDALKAIENGVIKPNSQDA
                     IITNPPFGSIKENGKTTKVEVDGYKIGKIDHLIAAEALKAMKPNGTAAIIIGADMVQG
                     GISTDDRIFFNWLYSNYNVVSHFEVDGKLYSRQGASWPVRVIIVHGKQESNSNSPVTG
                     AIPRYDNWKDVYDQYIKMVVASNNGGLSVGTRGSDNRIGNDTATTQPIDGVETTQTGA
                     RGRNTTRNNDDVDGASTRNVSNRTGNRSKQLRDSDNVQRFDERTNVQDNLAVGDNRDT
                     GGATRDNSGVGRGSKPNEHTGKDVAVLSDADNSFQVKYTPASSRKDEGVLIPINMRQP
                     LMESLLTLEDSVGDIDKYAAKELGYRNVEQLHDALMGLQVDSVASAIYQIKEKNKGII
                     IADQTGIGKGRQAAAIIRWAILNGHTPVFVTVKPQLFTDMYGDLSDIGSNDVKPFIVN
                     TGESISGKDGQKLHKTKPDAKHRKEIEDIASDGVLPDEYNAVFITYSQINKANNQQRL
                     LSSLSGNAIFILDESHNAGGASATGLFVQDLLKEAKGVVYLSATFAKRPDNMPLYFKT
                     DMGMAVGETSELSAAVIQGGLPLQTVISNSLVKAGQLFRRERSYNGVSIETKVDSQNK
                     LAHEKLSDEATKALRAIVAADKMFHNTYFARLEKAMEEQGQQAKAAGNKAEKSVSHHE
                     FSSVVHNFIRQMLLGLKVDTAANEAIAALKRGEKPLIALENTMGSFLNEYVSANGLKE
                     GDSLGNFGYRNVLSRALYRTRAVTIKDKKGKETRKEVGLDELDSETRAAYNAAQEIID
                     SLDISIPASPIDWMRNRLEEAGYSVAEITGRNMAVDYSTKTPTVSQVPLSEQNDKVGT
                     TRMFNNGELDAIILNVAGSTGISLHASEKFKDQRKRHMIVAQPAQDINIFMQMLGRIH
                     RTGQVILPKYTILSLDLPTEKRPTAVLNKKMKSLNANTSSNTESATSIVSLDMLNKYG
                     DKIVASYLSENQDVARKIGMEVHGDTSTSDTHDDFAKKATGRFALLPVSEQNRIYDEI
                     EPQYAALISYLDETGQNELEPKTVDFDAELKLEAIAVPATDPSTPFGQEAIYGEYKVK
                     PQGKPMSVDEVKALIVKNIGEGVMPAQHTQKLITDGQLAFDSFINTLNEKYKAAENEE
                     EAGIIQKNIVNQQAVFENTKSFIDSHRVGSIWQIDIKGDIYNAVITNVKSTFKKGAHG
                     NPFAQSKILISLAVNGELRTVTVPMTEFSRIEVTKYYGTAQDALKPREGDAGLAKIIT
                     GNLLAGYANLVGSDGRVISFTKKDGTTEQGILLPKKFNFKDNVRQDFAIKDANDAFKL
                     LKSDVTDILKTGVASRKGDIRIVHNTNTGGITIYTPKSKSTGGKWFLNKKLIDVVGDF
                     VSSGNMMVVNVPSKKAIDALSIVIEKTPLYVLPSQAEATKELLGYYDGQVNLKSGSNP
                     Q"
BASE COUNT         1211 a         1047 c          919 g         1660 t
ORIGIN      
        1 tcttgggggt tgctgcctga ttttagatta acctgaccat catagtatcc taaaagctct
       61 tttgttgctt ctgcttggct tggtaataca tataacggtg ttttttcaat aacgatggaa
      121 agagcatcaa ttgctttttt tgatggaaca ttaaccacca tcatattccc actactaaca
      181 aaatcaccga caacatcaat aagtttttta ttcaaaaacc attttccgcc tgttgatttt
      241 gattttggag tgtaaatagt aataccgcca gtattagtgt tatgaacaat ccgaatatca
      301 cccttacgac ttgctacgcc cgttttgaga atatccgtaa catccgactt caatagttta
      361 aaggcatcat ttgcatcttt aatagcaaag tcttggcgaa cattatcttt aaagttgaat
      421 tttttaggca acaaaatgcc ttgttcggtt gtgccgtctt ttttggtaaa tgaaataaca
      481 cgcccatctg acccaacaag atttgcatat cccgctaaaa gattgccagt aatgattttg
      541 gccaatcctg cgtcaccctc tctcggcttt aacgcatctt gcgctgtgcc gtaatacttc
      601 gttacctcga tacgagaaaa ctcggtcatt ggcactgtga cagttcttaa ctcgccatta
      661 accgccagtg agataagtat tttgctttgt gcaaatggat taccatgcgc tccctttttg
      721 aaagttgact taacattggt gataaccgca ttgtaaatat cgcccttaat atcaatctgc
      781 caaatactgc cgacacgatg gctatcaata aatgattttg tgttctcaaa tactgcttgc
      841 tgattcacaa tatttttttg aataatgcca gcttcttctt cgttttcggc tgctttgtat
      901 ttttcattaa gcgtgttaat aaaagaatca aacgccaatt gaccgtcagt aattagtttt
      961 tgtgtatgct gtgcaggcat aacaccttcg cctatgtttt tgacgattaa agccttgacc
     1021 tcatcaacac tcattggctt gccttgcggc tttaccttgt actcgccata aatagcctct
     1081 tggccgaatg gtgtacttgg gtctgttgca gggacagcta ttgcttctaa ttttaattca
     1141 gcatcaaaat caactgtctt tggctcaagc tcattttgtc ctgtttcatc aagatagcta
     1201 attaaggcgg catattgtgg ctcaatctca tcataaattc tgttttgctc ggaaactggc
     1261 aacaatgcga atctacccgt agcttttttt gcaaaatcat catgcgtatc ggacgttgat
     1321 gtatctccat gaacctccat gccaatcttt cttgcaacat cttgattctc gctaaggtaa
     1381 gaggcgacta ttttatcgcc atacttatta agcatatcaa gcgaaacaat agatgttgcc
     1441 gactcggtat tagatgaagt atttgcattg agtgacttca tctttttatt aagtacggct
     1501 gttgggcgtt tttcggttgg taagtccaaa gaaagaatag tgtacttagg caatatcact
     1561 tggccagtac gatgaatacg cccaagcatt tgcataaaga tgttaatgtc ttgtgctggt
     1621 tgagcaacaa tcatgtgccg tttgcgctgg tctttgaact tctcggatgc gtgtaagcta
     1681 atgcctgttg accctgccac atttaagata attgcatcaa gctcgccatt attaaacatt
     1741 cgtgttgtgc ctaccttgtc gttttgttct gacaatggca cttgagaaac ggtaggtgtt
     1801 tttgttgagt aatcaacagc catattacgg cctgtaatct cagcaacact atatcctgcc
     1861 tcctctagtc tgtttctcat ccagtcaata ggtgatgctg ggattgaaat atccagcgag
     1921 tcaataattt cttgtgctgc gttataggct gctcttgttt cgctatctaa ttcatccaat
     1981 cctacttctt ttctagtttc tttgcctttt ttatccttaa tggttacggc tcttgttcga
     2041 tacaaagcac gacttaatac atttctgtag ccaaaattgc caagcgaatc accctctttt
     2101 aaaccgtttg ccgaaacata ctcgtttaag aacgaaccca ttgtattttc taatgcaatc
     2161 aatggttttt cgccacgctt taacgcggca atggcttcat tagcggcagt atccacttta
     2221 aggccgagta gcatttgtct aataaagtta tgaaccacac tagagaattc atggtgacta
     2281 acagatttct ctgctttatt tcctgctgcc tttgcttgtt gtccctgctc ctccattgct
     2341 ttttctaatc gcgcaaagta agtgttatga aacattttgt cggcggcaac aattgctctt
     2401 aatgcttttg ttgcttcatc cgataatttt tcgtgtgcta atttgttttg agaatcaacc
     2461 ttcgtttcaa tgcttacgcc gttatagctt ctttcgcgtc taaataactg gccagccttg
     2521 actaatgagt ttgaaataac tgtctgtagc ggcaatccac cctgtattac tgctgcgcta
     2581 agttcgcttg tttcgccaac agccattccc atatcggttt taaagtaaag cggcatattg
     2641 tcaggacgtt tagcaaaagt tgccgataaa taaacaaccc ctttggcttc ttttagtaaa
     2701 tcctgaacaa acaaacctgt agccgatgca ccaccagcat tatgtgattc atcaagaata
     2761 aaaatcgcgt tacctgataa agaggaaagt aaccgttgct gattgtttgc tttattgatt
     2821 tgagagtagg tgataaatac cgcattgtat tcatcgggca atacgccatc ggaggcaata
     2881 tcctctatct cttttctgtg cttggcatct ggttttgttt tgtgtaattt ttgaccgtct
     2941 tttcctgata tggattcccc agtattaaca ataaatggtt ttacgtcatt actgccaatg
     3001 tcggataaat caccatacat atcagtgaat aattgcggtt taacggtaac aaaaacaggc
     3061 gtatggccat taagaatagc ccatcgaata attgctgctg cttggcgacc tttaccgata
     3121 cctgtttggt cggcaatgat aatgcctttg tttttttctt tgatttgata aatggctgac
     3181 gcaacagaat caacctgtaa tcccattaaa gcatcatgca attgctctac attacgataa
     3241 ccaagctctt tagcggcata tttatcaata tcgccaacac tatcctcaag ggttaataga
     3301 gactccatga gtggttgacg catattgata gggataagca ctccctcatc tttgcgtgat
     3361 gatgcaggtg tgtattttac ctgaaaagag ttatcagcat cggataatac agctacatct
     3421 tttccagtat gctcattagg ttttgaccct ctacccactc ctgaattgtc tcttgttgct
     3481 cctcctgtat ctcggttgtc tccaacggcc aaattatctt ggacattagt gcgttcatcg
     3541 aatcgctgta cattatcgct gtcgcgtaat tgcttacttc tgtttcctgt gcgatttgat
     3601 acatttcggg tagatgctcc atcgacatca tcattgtttc ttgtggtgtt tcgtcctctt
     3661 gcaccagtct gcgtagtttc gactccatca ataggctgtg tagttgcggt gtcatttccg
     3721 attctgttgt ctgaaccgcg agtaccgact gatagtcctc cgttgtttga ggccaccacc
     3781 attttaatgt attggtcata tacgtctttc cagttgtcgt aacgtgggat tgcgcccgtt
     3841 actggtgaat tgctattcga ttcttgtttg ccgtgaacaa taattacccg tacaggccaa
     3901 gatgcgccct gtcttgagta tagcttacca tcaacttcaa aatgactaac aacattgtag
     3961 ttgctgtata gccaattaaa gaaaatacgg tcatcggttg atataccgcc ctgaaccata
     4021 tccgcaccga taataatagc agccgttccg ttaggcttca ttgcttttaa tgcttcggct
     4081 gcaattaaat ggtcaatctt gccaatttta taaccatcaa cctcaacttt agttgtttta
     4141 ccgttttctt taattgagcc aaacggcggg ttagtgatga ttgcatcttg agagttaggc
     4201 ttaattacac cgttttcaat ggcttttagt gcatcgcctt gatgtgcatt aaatccttgt
     4261 gcttttaggt tattgaatcg gatgctttcc atttcgttta cggtagcgtt ttcaggcaat
     4321 gctgtaatca gcaacattcc attgcctgct gttggctcat aaacctttgt gcttttgcca
     4381 atgttagcca tttttgcagc aacaaatgct aaaggcgcgg gtgtgctata ggcttggttc
     4441 tcaacactgg tgcttgttct gatattcagg tttggctgct tgttgtaaga atcaaccaat
     4501 tgattaaatg ttgaagcatc accgtttttg tttgatgtca caacatcacg cgagtagcga
     4561 gccattgccg cctcaagcat ttcttgtgct tcttttaaac ggtaattatc gacttctttt
     4621 ccgtcaaatt cggccaccat cttacgcaaa gcagcattgt tttcaggcaa cgtccccttt
     4681 tttaaatggt cgtagaatag gtcggttaat gtttttttgg ttgtttgtgt cttttctatg
     4741 ctgtcggctg cttcttcttg acctacatta agtgtagcaa agtcaaaatc atatacatca
     4801 tcctctttat ccatttgttg acggatttcg ggtgata
//
LOCUS       DAOQMB010000154         4819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8286_length_4819_cov_14.162678, whole genome shotgun sequence.
ACCESSION   DAOQMB010000154 DAOQMB010000000
VERSION     DAOQMB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8286_length_4819_cov_14.162678"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLY05_06415"
     CDS             complement(<1..1055)
                     /locus_tag="PLY05_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC02974.1"
                     /translation="MVHLPKDETNLILTTETTADNAHSRRDFLKQSAAAAGVGLLGGG
                     IMSLSSSAWAAGSDKPEKTEVKVGFIPLTDCAPIVVASVLGFDKKYGIKITPSKEASW
                     AGVRDKLNNGELDAAHVLYGLIYGVQLGIGGPAKDMAVLMTLNQNGQAITLSNQLKEQ
                     GVVDGRSLKRMIDSNKREYTFAQTFPTGTHAMWLYYWLAAYGIDPMNDVKTIVVPPPQ
                     MVANMRVGNMDGYCVGEPWGARAISDGIGFTTATTQEIWPNHPEKVLGCTKAFVTQYP
                     NTARAMLMAVLEASRYIDDVKNRPAVAKLIADKSYVNAPEQVIEQRFLGNYDNGIGRK
                     WKDANPMQFYRNGEVSFP"
     gene            complement(1585..2160)
                     /locus_tag="PLY05_06420"
     CDS             complement(1585..2160)
                     /locus_tag="PLY05_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HNC02975.1"
                     /translation="MLKVLLVNDTNKQVGRLRTALIQAGCEVVEEVTSALMIPKRVAE
                     LKADVVIIDTESPSRDVLEQICVVTQDEPRPIVMVSEDTQPAAIKAAIKAGVSAYVVE
                     DIDVARLSAVLEVAQARFEQDQALMSQIRQAESKLNERKVVERAKGILMQLRGMTESE
                     AYHAMRKMAMDRNIRIVDVAQKLLAMNDLLA"
     gene            complement(2242..2832)
                     /locus_tag="PLY05_06425"
     CDS             complement(2242..2832)
                     /locus_tag="PLY05_06425"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HNC02976.1"
                     /translation="MTQHSQIKADPLTIARLLQDEQGNVPKGLPPIHKWHPPYCGEID
                     MQIKANGEWFYQGTPIQRHALVKLFSTILRKEEDGHFYLVTPVEKVRIQVEDAPLLAS
                     HVERVVENGVAYLRFKTLTEDVVVIDEQHALWVNYNDAGEPRPYIRVRDQLDALLTRS
                     VFYQLVEWAECIDLQDKSCYAIASAGQYFYLADVFD"
     gene            3182..4819
                     /locus_tag="PLY05_06430"
     CDS             3182..4819
                     /locus_tag="PLY05_06430"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005316675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HNC02977.1"
                     /translation="MEFDVVIVGGGPAGLSAAIRLRQLSIESGHDISVCLVEKGSEIG
                     AHILSGAVFEPRALNELFPNWKEEGAPLNTPVIKDEIHLLFSETRSKLMPDWAVPKAM
                     HNEGNYVISLGNLVRWLGTKAEELEVNLFPGFAASEILYHEDGSVKGVATGDMGIGKN
                     GQKKHSYTAGYELHAKYTLFAEGCRGHLGKQLISKFELDKDRDPQHYGIGIKELWEID
                     PSKHKPGLVLHGSGWPLSESGSAGGWFLYHAENNQVTLGLITDLAYSNPYLSPFDEMQ
                     RWKHHPVIKHYLEGAKRVSYGARAIIKGGLNSLPKLTFAGGCLIGDDAGFLNFAKIKG
                     SHTAMKSGMLCAEAVFEALKDGREGHDEVTTYTEKFENSWLYEELHRYRNFGPAMHRM
                     GFLMGAAFNFIDQNILPSGFPFTIHDNKPDYDCLDRKDLARKLEYPKPDGKISFDKLS
                     SVFISGTNHEEDQPIHLQLKDPSIPMSKNLPLYDEPARLYCPAGVYEVVSLAGEQRLQ
                     INAQNCVHCKTCDIKDPSQNINWVTPEGGGGPTYPNM"
BASE COUNT         1475 a         1049 c          866 g         1429 t
ORIGIN      
        1 gggaatgata cttcaccatt gcgataaaat tgcattgggt tggcatcttt ccatttacga
       61 ccaataccat tgtcataatt acctaagaaa cgttgttcaa tcacttgttc aggtgcattc
      121 acatacgatt tatcagcaat taatttagca acggctggac gattcttgac gtcatcaata
      181 taacgactag cttctaatac agccattaac atagcacgtg cagtgttggg atattgcgtt
      241 acaaaagctt tagtacaacc taagactttt tcgggatgat ttggccaaat ttcttgagtg
      301 gttgcggtag taaaaccaat gccatcactg attgcacgcg ctccccacgg ttcacccaca
      361 caataaccat ccatattacc gacacgcata tttgcaacca tttgtggagg tggcacaaca
      421 atggttttga catcattcat tggatcaata ccataagcag ctaaccagta gtacaaccac
      481 atagcatgtg tacccgttgg aaaggtttgt gcaaaggtat attcacgctt attagaatca
      541 atcatgcgct ttaatgaacg accatcaacc actccttgtt cttttaattg attcgataaa
      601 gtaatcgctt ggccattttg attaagagtc attaacacgg ccatatcctt agcaggccca
      661 ccaatgccta attgcacacc ataaatcaaa ccatataaca catgagcagc atctaattcg
      721 ccattattta atttatcgcg gacacctgcc caagaagctt ctttgcttgg ggtaattttg
      781 ataccatatt ttttatcaaa acctaaaact gatgccacaa caataggcgc acagtcagtt
      841 aagggaataa aacccacttt cacttctgtt ttttcgggtt tatcactacc tgctgcccat
      901 gcactgcttg ataagctcat tattccgcct cctaataacc ccactcctgc cgcagctgcc
      961 gattgtttta aaaaatcacg gcggctatga gcattatcag cagtagtttc agttgttaaa
     1021 attaaatttg tttcgtcttt aggcaagtgc accatgatca tcaacctcaa tcttggtaac
     1081 gccacctcta gatttattag gtgtttatac aattccaatg ccaacacacc ataaataacc
     1141 aaggttttgt tacatgattt attaaaaaga gttttttatc ctcaaaaacc ctaacaaaca
     1201 cccaaaaaaa aagcgtcctt cggctgtgaa gcctccagac gccagtgtcc caacttgtcg
     1261 ttgacctatg tactcacata agtcagcaaa tctgtattaa cttaaaggga ttttaatgat
     1321 ccactttaat tgcgtagcca actttggcta tttatcgctg tcacaatata tacagcaact
     1381 gttgtgccaa gtttttgacc ccatataaaa atcgtctttt cactatttag gtgcacgttt
     1441 tgcaccatga tagtgaatgg tgcacaaata aaagtggcta cttattaaat gatgcactca
     1501 aataacgtaa atccttgtta taaatgcgcc aaaataaaaa accgccctaa cggcggtttt
     1561 ttgaagtgct atttaattta ttttttaggc taataaatca ttcatcgcta aaagcttttg
     1621 agccacatca acaatccgaa tattgcgatc catcgccatt ttacgcatag catgataagc
     1681 ctcagactca gtcattcctc gtaattgcat taagatacct ttagcacgct caacaacctt
     1741 acgctcattt agtttagatt ctgcttgacg aatttgactc atcaaggctt gatcttgctc
     1801 aaaacgagct tgtgcgacct ccaaaacagc acttaaacga gctacatcaa tgtcttcaac
     1861 cacataagct gatacgcctg ctttaattgc tgctttaata gcggcaggtt gtgtatcttc
     1921 actcaccata acaataggcc ttggctcatc ttgagtcacc acacaaattt gctctaacac
     1981 atctcggcta ggtgattcgg tatcaataat caccacatct gccttcagtt ctgcgacacg
     2041 cttaggaatc atcaatgctg atgttacttc ttcaacaacc tcacatcccg cttgtattaa
     2101 ggcagtgcgt aaacgaccca cttgtttatt ggtgtcgtta accaacaaaa cttttaacat
     2161 tgccttgctc caaaactgct aaataaaaag ctttaaagca aagactatgc caaacacaaa
     2221 aaaaacatca aactttaaat cttaatcaaa aacatctgcc aaataaaagt attggcctgc
     2281 actagcaatg gcataacaag atttatcttg taaatcaata cattctgccc actcaactaa
     2341 ttgataaaaa acgcttcgtg ttagcaacgc atccaattga tcacgaactc tgatataagg
     2401 tctaggctcg cctgcatcat tataatttac ccatagcgcg tgttgctcat caataaccac
     2461 aacatcttct gtcaaggttt taaaacgtaa ataggcaaca ccattttcaa caactctttc
     2521 cacatgtgat gctaataatg gcgcatcttc aacctgaata cgaaccttct ccacaggtgt
     2581 cactaaataa aaatgaccat cttcttcttt acgcaaaatt gtactaaaca atttgactaa
     2641 ggcatgtcgc tgaatcggtg tgccttgata aaaccattca ccattcgctt tgatttgcat
     2701 atcaatctca ccacaatacg gtggatgcca tttatgaatc ggtggcaaac ctttaggtac
     2761 attaccctgt tcatcttgca acaagcgcgc aatagttaaa gggtctgctt tgatttgtga
     2821 atgctgagtc ataacaattc ctgtagattg tctaacaaaa tatcgtataa aacttgtaaa
     2881 ctaccgcaac aatacaaaac gcttatcata aaagcgaatg attgacttat ttagccgtaa
     2941 cttcatcgtg cttagccatt gaacaagggc aagaccacac gttaagttaa gcaaagataa
     3001 gcatttatag gtgtattatt ggtggtttta accaaaattg cgattaattt gcgatttttg
     3061 gccaacattc tatcgtaaag agttggcctt tataccctat gcttttatac tatcgcgcct
     3121 aaaattttag actagcttac cccagctaaa ttgcgaggag attaacgtgg aaagagaagc
     3181 aatggaattt gatgtcgtca tcgttggtgg cggccccgct ggtttatctg cagcgattcg
     3241 tttacgtcaa ttaagcattg aatctggcca tgatatctcg gtatgtctgg ttgaaaaagg
     3301 ttctgaaatt ggcgcacata ttttatctgg tgcagtgttt gagccacgcg ctttaaatga
     3361 attatttcca aattggaaag aagaaggcgc accactcaat acgcctgtta tcaaagacga
     3421 aattcactta ttgtttagtg aaactcgtag caaacttatg cctgattggg ctgtaccaaa
     3481 agccatgcac aacgaaggta actacgttat tagcttaggt aacttagttc gttggttggg
     3541 tacaaaagcg gaagaattag aagttaacct ctttcctggt tttgccgctt ctgaaatttt
     3601 ataccatgaa gatggtagcg taaaaggtgt tgcaactggt gatatgggta ttggcaaaaa
     3661 tggccaaaag aaacactctt acaccgcagg ttatgaatta cacgctaaat acaccttatt
     3721 tgctgaaggt tgccgtggtc atttaggcaa acaattaatc agcaagtttg aattagacaa
     3781 agaccgtgac cctcaacatt acggtattgg tattaaagaa ctttgggaaa ttgacccaag
     3841 caaacacaaa ccaggtttag tattacacgg ttctggctgg cctttaagtg agtctggtag
     3901 tgcaggcggt tggttcttat accacgctga aaacaatcaa gtcactttag gtttgattac
     3961 tgacctcgct tatagcaacc catacttatc accgtttgat gaaatgcaac gttggaaaca
     4021 ccatcccgtt atcaagcatt atttagaagg tgcaaaacgt gtttcttatg gtgcacgtgc
     4081 cattattaaa ggcggcttaa actccttacc taaattaact tttgctggtg gttgtttaat
     4141 tggtgatgat gcaggcttct taaactttgc caaaatcaaa ggctctcaca ccgctatgaa
     4201 atctggtatg ttgtgtgccg aagcagtatt tgaagcctta aaagatggcc gtgaaggtca
     4261 tgacgaagtc actacttata ccgagaaatt tgaaaattct tggttatatg aagagttaca
     4321 ccgctaccgt aactttggcc cagccatgca ccgcatgggc ttcttaatgg gtgctgcctt
     4381 taactttatc gaccaaaaca ttttgccgag tggtttcccg ttcactatcc atgacaacaa
     4441 accagactat gattgtttag atcgtaaaga tttggcgcgt aaattggaat atccaaaacc
     4501 agatggcaag atcagttttg ataaactgtc ttctgtgttt atttctggta ctaaccacga
     4561 agaagatcag ccaattcatt tacagctcaa agaccctagc attccaatga gcaaaaactt
     4621 gccattgtat gatgagccag cgcgtttgta ctgcccagca ggtgtgtatg aagtggtttc
     4681 tttagcaggt gaacaacgtt tacaaatcaa tgcgcaaaac tgcgtacatt gtaagacttg
     4741 tgatattaaa gacccctctc aaaacatcaa ctgggtaaca ccagaaggtg gtggtgggcc
     4801 tacatatcct aatatgtaa
//
LOCUS       DAOQMB010000155         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8291_length_4816_cov_16.616677, whole genome shotgun sequence.
ACCESSION   DAOQMB010000155 DAOQMB010000000
VERSION     DAOQMB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8291_length_4816_cov_16.616677"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..2360
                     /locus_tag="PLY05_06435"
     CDS             366..2360
                     /locus_tag="PLY05_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT and VWA domain-containing protein"
                     /protein_id="HNC02978.1"
                     /translation="MRALLQKTILGVAISTSVGVSQSTWAAGMLQVAGDKNQAIRIID
                     HDVAVTINNGFAKVEVTQRFANPNSDTVEAVYNFPLPKSASLSEMSILSGETRLNGEV
                     LPKKQAETIYQEQKSQGKEAGLAKQNSYKSLDFKVYPLMPKQDITVRFVYYQPVVLDT
                     GVGRFVYPLSEGGTDEAGQQFWAKNEQVDNSFSIDINVKSAWPIDSVRAPNLEQVAQI
                     SHKNAYQWQLKAEQMGKSLNQDVVFYYRLADNLAGRVEVIPYKAAKDKEGTFMLVVTP
                     GVDLKPLNAGADYTFVLDVSGSMDTKLATLIKGIEQTLGKMTGNDRFRVVAFNDTAQQ
                     VIPMTLATPDNVQRAIEQLKNLRSGGGTNLYSAVREGLSQLNADRVNNMILVTDGVTN
                     QGLLEPKAFADLVKQYDLRIFAFVMGNNANWPLVRLIADASGGFYAGVSNDDDIVGQV
                     LLAKSKVTSEALHHVDLTMDGVATYDMVGNLGKKIYRGQQVVMFGHYQQAGKVKLNLQ
                     ASLSGQDKTYSTEFNLPEVATANPELERLWALAKVEDVQKLSMLGVIPEEKAQKAVQD
                     LGVKYQIVTEETSMVVLKDDAFASRRIARQNQQRIAVEQQAQSVTAQQAPQNYRVDNS
                     QPTFNRPAHSLSRGGGGGAANPLMLLVLGLLAIFSFKRRC"
     gene            2505..2789
                     /locus_tag="PLY05_06440"
     CDS             2505..2789
                     /locus_tag="PLY05_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HNC02979.1"
                     /translation="MYNDQGIQQDSGSWIFFVKACFVCAIGALGGGIFLLPADMWIKG
                     YLLMGMLFTVGSTFTLAKTLRDQHEAQKLIHKISNAKTEKMLKELEMTGG"
     gene            2857..4200
                     /gene="yegQ"
                     /locus_tag="PLY05_06445"
     CDS             2857..4200
                     /gene="yegQ"
                     /locus_tag="PLY05_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HNC02980.1"
                     /translation="MTPELLLPAGSLDKMRTAYDFGADAVYAGLPRYSLRARNNEFRL
                     EQLSQGINEAHQRGKQFYVTANILPHNDKVRSFVRQLQPVVDMKPDAFIMADPGLIML
                     AREAWPDVNIHLSVQANTVNYAAVKFWQSVGVKRIILSRELSLDEIAEIRQQCPDMEL
                     EVFVHGALCIAYSGRCLLSGYFNRRDPNQGTCTNACRWNYKMHDAEENDSGDIVKLQG
                     FDFNKELEEANNNFSACGGTERHPEADKIYLIEEASREGELMPIMEDEHGTYIMNSKD
                     LRAIEYVDKLAKIGVNSLKVEGRTKSLYYVARTAQTYRRAIDDAVAGKPFNPELLLEL
                     EGLSNRGYTAGFLQRHASQDTQNYITGNSEAKRSQFVGQVLSVEEGWGVVEVKNHFEL
                     QDRLEIIHPQGNLQITLEKMLNTKGEAIQVAAGSGLTVKIPLDASYQGALLARLF"
     gene            4342..>4816
                     /locus_tag="PLY05_06450"
     CDS             4342..>4816
                     /locus_tag="PLY05_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02981.1"
                     /translation="MNMTYEFDAKLPIRDRQNLTLVRHTKQQMQLWVQRLPVNPLECG
                     KELYQVLTELTTLNIEAELRLELLSVLQMPVYTLIAALEKFYLQQPLLLAIQGKRTLA
                     LAQTLRQNLAINYKIIAVETTEKLKGKMGFLDFGRKAGQSLAATAIQHYILQAQRI"
BASE COUNT         1499 a          841 c         1054 g         1422 t
ORIGIN      
        1 ttcctaaaat ttgtttttct aaagcaatcc gttcttcacc aaaagggtta gtaaaaccta
       61 aattgcctaa agctttggct ttatgtagtt gagcagggct aaagagtgta tttgtcatct
      121 aaaaaaaact caatttgact tcgactacgc tcagtccaca caagtgtact ctgagcgtag
      181 tcgaagagtt tatcctacaa aatatgttta ttgctcttgc aaaatatgta tatcagtttt
      241 aatggctttg gcaaatgaaa agttgtgata aatgtatcta aatgattttt atcaataaaa
      301 ttataaaaaa tagcttggca tagatttggc tttatgaata acaactttat aaggagcaaa
      361 taacgatgcg agctttattg caaaaaacaa ttttaggtgt tgccattagt accagtgtcg
      421 gtgttagtca aagcacatgg gcagcaggta tgttgcaagt ggcaggtgat aaaaaccaag
      481 cgattcgaat tattgaccac gatgtggcag tgaccattaa taatggtttt gccaaagtcg
      541 aagtgaccca acgttttgca aaccccaata gtgatacggt tgaagcagtt tataactttc
      601 ctttgcctaa aagtgctagc ttgtcagaga tgtccattct gtcaggtgaa acacgtttaa
      661 acggcgaagt tttacccaaa aagcaagctg aaaccattta tcaagagcaa aaaagccaag
      721 gcaaagaagc aggcttagcc aagcaaaaca gctacaaaag tttagatttt aaagtttatc
      781 ccttaatgcc aaaacaagat atcaccgtac gttttgtcta ttaccaaccc gttgttttag
      841 atacaggtgt gggacgtttt gtttatccat tgtctgaagg cggcacagac gaagcaggcc
      901 aacaattttg ggctaaaaac gaacaagttg ataactcttt ctcgatagat attaatgtca
      961 aaagtgcatg gccgattgac agtgtacgtg cgccaaacct cgaacaagtg gcacaaatca
     1021 gccataaaaa tgcctatcaa tggcaactta aagccgagca aatgggcaaa agtctgaatc
     1081 aagatgtggt gttttattat cgtttggccg ataaccttgc agggcgtgtc gaggtgattc
     1141 cctataaagc agctaaagat aaagaaggca cttttatgtt ggtggtcaca cctggtgtgg
     1201 atttaaaacc cttaaatgca ggcgcggatt acacctttgt tttggatgtg tcaggcagca
     1261 tggacaccaa actggcgact ttaattaaag gtattgaaca aaccttgggc aaaatgacgg
     1321 gtaatgatcg ttttagagtg gttgctttta atgataccgc acaacaagtg attccgatga
     1381 ctttggccac gccagataat gttcaacgtg cgattgagca attaaaaaat ttacgcagtg
     1441 gtggtggtac taatttgtac agtgcggtga gagagggttt gagccaatta aatgccgatc
     1501 gtgttaataa catgattttg gtgactgatg gtgtgaccaa tcagggcttg cttgaaccca
     1561 aagcttttgc tgatttggtg aagcaatatg acttgcgtat ttttgccttt gtcatgggta
     1621 ataatgccaa ttggccatta gtccgcttaa ttgctgatgc ttcaggtggt ttttatgcgg
     1681 gtgtgtctaa tgatgacgac atcgttggtc aagtgttatt agccaaaagt aaagtgacca
     1741 gtgaggcttt gcatcatgtc gatttaacaa tggatggcgt agccacttat gacatggtgg
     1801 gtaatttagg taaaaaaatc tatcgtggcc agcaagttgt catgtttggt cattatcaac
     1861 aagcaggcaa ggttaagctg aatttacagg ctagtttaag tggtcaagac aaaacctata
     1921 gcaccgaatt taatctgccc gaagtagcaa cggccaaccc tgaactagag cgtttatggg
     1981 cattggctaa agttgaagat gtacaaaaac tgtcgatgtt gggggtgatt cctgaagaaa
     2041 aagcccaaaa agccgttcag gatttgggag tgaaatatca aattgtcacc gaagaaacca
     2101 gcatggtggt gttaaaagat gatgcttttg ccagccgtcg tattgcccgt caaaaccaac
     2161 aacgcattgc tgttgaacaa caagcacaaa gtgtaactgc ccaacaagct ccacaaaatt
     2221 atcgtgttga taacagtcaa ccaacgttta atcgacctgc acatagttta agccgtggtg
     2281 gtggtggcgg agcagccaat ccattgatgt tgttagtatt aggtttgttg gcgatatttt
     2341 cgttcaaacg ccgttgttaa aaacagcaaa atatgtatgt aagataaaca ttatttgtat
     2401 gtttgatttg gtgtttatct tgcatcttat tgaattaaat ataaaaaata gttggcatat
     2461 atttggctat atataagata acaataatca acgaggtgtt tatcatgtac aacgatcaag
     2521 gcattcaaca agactctggt tcttggatat tttttgttaa agcctgtttt gtctgtgcga
     2581 ttggggcttt aggtggtggt atttttttgc tacctgctga tatgtggatt aaaggttatt
     2641 tgttgatggg gatgttgttt actgttggct caacattcac tttagcaaaa actttgcgtg
     2701 accaacacga agcgcaaaaa ttgattcata aaatcagcaa tgcaaaaacc gagaaaatgt
     2761 tgaaagagtt ggaaatgact ggtggataaa taaaaactat ccctcaaaat ttgtaggggt
     2821 taaaatagcc aaaatttttg tagagagttt ttagctatga cccccgaatt attgttacct
     2881 gcgggtagcc ttgacaaaat gcgtactgct tatgactttg gtgcagatgc cgtgtatgca
     2941 ggtttacctc gttactcttt acgtgcgcgc aacaacgaat ttcgtttaga acaattatcc
     3001 caaggtatta acgaagccca tcaacgtggc aaacaatttt atgttaccgc caatattttg
     3061 ccgcataacg acaaagtacg cagttttgtt cgccaattgc aacctgtcgt tgatatgaag
     3121 cctgatgcct ttattatggc tgacccaggt ttgattatgt tggcacgcga agcatggcca
     3181 gacgtgaata ttcatttatc ggtacaagcc aacaccgtta actatgcagc ggttaagttt
     3241 tggcagagtg tcggtgttaa acgcattatt ttatcgcgtg aattatcgtt agatgaaatt
     3301 gccgaaattc gtcaacaatg cccagacatg gaattagaag tgtttgtcca cggtgcatta
     3361 tgtattgctt attcgggacg ctgtttgttg tcaggctatt ttaatcgtcg tgaccccaat
     3421 caaggcactt gcaccaatgc ttgtcgttgg aattataaaa tgcacgatgc cgaagaaaat
     3481 gattcgggtg atattgttaa attacaaggc tttgatttta ataaagagtt agaagaagcg
     3541 aataataact tttcagcgtg tggtggcaca gagcgtcacc cagaagcgga taaaatttat
     3601 ttaattgaag aagctagccg tgaaggcgaa ttaatgccga ttatggaaga tgaacacggc
     3661 acttatatta tgaactccaa agatttgcgt gccattgaat atgtggacaa attggccaag
     3721 attggtgtca attctttaaa agtcgaaggc cgaaccaagt ccttgtatta tgttgcaaga
     3781 accgcacaaa cctatcgtcg agccattgat gatgcagtcg caggaaaacc gtttaatcct
     3841 gagttgttat tagaattaga aggtttatca aaccgtggtt ataccgcagg tttcttgcag
     3901 cgtcatgcgt ctcaagacac acaaaactat attacaggta actcagaagc caaacgtagt
     3961 cagtttgttg gccaagtctt atcggttgaa gaagggtggg gcgttgttga ggttaaaaat
     4021 cactttgaat tacaagaccg tttagaaatt attcatcctc aaggcaattt gcaaattacc
     4081 ttagaaaaaa tgctaaacac taaaggcgaa gcgatacaag tggctgcagg cagtggctta
     4141 acagttaaaa ttcctttaga tgccagttat caaggtgcac tcttagcaag gttgttttaa
     4201 acgcattcta tcgccaaatt tgagcatttc gtcgttaaac tttgaggagt tttgatgaaa
     4261 tgcttaaact atcttatgaa aagtgtgata taacacacaa aaattataac gatatagggc
     4321 cgtgttgtag atattacagt gatgaatatg acctatgaat ttgatgccaa attacctata
     4381 cgtgatcggc agaatctaac gttagtaaga cacacgaagc agcaaatgca attgtgggta
     4441 cagcgtttgc ctgttaatcc tttagagtgt ggcaaagaat tgtatcaagt gttaacggag
     4501 ctaacgacct taaatataga agctgaatta cgtttagagt tgctcagtgt tttacaaatg
     4561 cctgtttata cactgattgc tgcattagaa aagttttatt tacagcagcc tttattatta
     4621 gccattcaag gaaaacgcac tttagcctta gctcaaactt tacgtcaaaa cttagccata
     4681 aactacaaaa ttattgcggt tgagaccact gaaaaactta aaggcaaaat gggcttttta
     4741 gattttggac gtaaagcagg acaaagttta gccgcgacag ccattcaaca ttatatttta
     4801 caagcgcaac gtattt
//
LOCUS       DAOQMB010000156         4809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8311_length_4809_cov_14.214135, whole genome shotgun sequence.
ACCESSION   DAOQMB010000156 DAOQMB010000000
VERSION     DAOQMB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8311_length_4809_cov_14.214135"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4405)
                     /locus_tag="PLY05_06455"
     CDS             complement(<1..4405)
                     /locus_tag="PLY05_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC02982.1"
                     /translation="MAIFVYITEKCQQRAKNHQLTDALDQLVADIEREQAFWRLDAFP
                     HPFWVKKRLGNRHTRLVCRLESHEIEGETHDVLVCLDIFQRGDKEYHKLYLQIRDLGD
                     VLYQQVTDPQLLITWLKQRCQQPMLPNLAKPTEEEDLFLYAVPSSTNHHEYGNIKDMV
                     YESWSWVEQGLKLCSPQQLEEISQQLAAWDQKTACLLLELEMGEAWILPFVQHHVCLL
                     TFNTKELVFTIQDLMQKGETLNIHDFQKFLVCAARRVYPQSLLKIPNQWLSVQHNFAA
                     NLAFSPEESHILHFTLQEERPFPLFINGRAGSGKSTILQYLFSGYLNFAYQEICSSKS
                     PIYPVYFTCNQTLTDRAAETINQILLACNPQQAWLPIIKTVCLEFRGFLLKLMPTEQQ
                     SLFGREKWLDYGRFRRLWEQKFGQQPQATQEYPASLCWHIIRSYIKGLNADYYLEPSD
                     YLDLAEDQRSVSDELYQTIYEKVWEKWYQPLCKKEGYWDDQDLARFVLEQYYVKPRFA
                     AVFCDEAQDFTRIEVQIVLRLNLFSARHLYPNQIQRVPLVFAGDQFQTLNPTGFRWET
                     IKAQVVEQFIFALDPAQHATLSDINYRELSYNFRSTPAIAQFSNIIHGLRAKLFGLKS
                     LVPQQSWQTSTNQRDVILIDTQDHTLTVQLAQQYDLVIVLPCLDGQETSYIQNNPILK
                     QYLHLREDGSTSIPAMSVIRAKGLEFQRVVIYGFAADCPVDLDLSGKALISAEQKIQQ
                     EYFLNRLYVAVTRARSQLLIIDSPTNNQQFWQHFQQPLSFPLQLMDLPNAQETWVEHL
                     GSLRAAVLADITPDANQILLMTENATKIYQEGIANQDPYLLRQAAGLYEQLGDYNQQQ
                     YCLAEVQYIAEDYYEAGILFEKTAHYERAFECFWRVQDWQKLYDVTTEQSWRPENIEN
                     KLREEMLELYIKKTSAIASYVNIIRKLVNHELPLSSNTYDKKAWHNLLTEILIQLPLD
                     KIDRHWKKLYNQLKLLDSHAYFSAVIHPAIFAEVAYLANVMDEACRWWEKAKEQYKLT
                     PPMPRYAEAVAVSRPFPKNLDALVVLQRNTAICQQFSDFLNIDDLSSHAWQLLLDVLF
                     RVLKNDELIALVNPKLLNLHRLDLLDALIDVIQQESSNYLKGWTKKLTKLRLFRAATQ
                     GDWTSVIEAFNQETEQDANGLTANIELILYGLAHSDAYSQMVIDDHLNTDAQTILQLI
                     KKRFLQQVVRQVSKQNHFKKGHSSQVSQEVSYVWRTDIKNPRLLGAVLERSQSFSDAL
                     QFYECLENNQRESHYAKRRWLACKLRQAEYFYKLSERYKDKLELSTRVQEREELESKL
                     RDTEQKAEQNQNTARREARLLGINNYDDIPQYPLLPTQEDIVNKVLEIKKDIVIIPET
                     QEPVVELVTEPVQNVSSSVVVEPTLEAVTVIDKPIIKQTLAIPFSKKSLSDAIVHEQE
                     LTSTLSVDVDYLHKGINDVMQATSLLSEMLAK"
BASE COUNT         1494 a          960 c          822 g         1533 t
ORIGIN      
        1 ttttagcaag catctccgat agcaagctag ttgcttgcat tacatcgttg atgcctttat
       61 gcaaataatc aacatccaca gataatgtac ttgttaactc ttgctcatga acaatggcat
      121 cagataacga ttttttgcta aacggtattg ctaatgtttg tttgatgatt ggtttgtcaa
      181 tgacagtgac agcctcaagt gttggctcaa ccaccacaga gctagatacg ttttggactg
      241 gctctgtgac caactctact actggctctt gggtttctgg tattatgacg atgtcttttt
      301 taatctccaa tactttattc acaatatctt cttgcgtagg caatagtgga tattgtggaa
      361 tatcgtcata gttattaata cccaacaaac gagcctcacg tctagctgta ttttgatttt
      421 gctccgcttt ttgctctgta tctcgcaatt ttgactctaa ttcctctcgc tcttgcactc
      481 gcgtacttag ctcaagcttg tctttataac gctcagacaa cttataaaaa tattctgctt
      541 gccgtaattt acatgccaac caacgccgtt tggcataatg gctttctctt tgattatttt
      601 caaggcattc gtaaaactgt aaagcgtcac taaagctctg actacgttct aatacagcgc
      661 ctaacaaacg aggatttttt atatcggtac gccacacata actcacttct tgagaaactt
      721 gcgaactatg ccccttttta aaatgatttt gtttactaac ttgtctcaca acttgttgca
      781 aaaaacgctt tttgataagt tgcaatatgg tttgagcatc agtgttcaaa tggtcgtcaa
      841 ttaccatttg actataagcg tctgaatgcg ccaagccata caatattaac tctatgtttg
      901 cggttaatcc gtttgcatct tgttctgttt cttggttaaa agcctctatc acagaagtcc
      961 aatcaccttg cgttgctgct ctaaaaaggc gtaatttagt gagcttttta gtccatcctt
     1021 tcagataatt gctcgactcc tgttgaataa cgtcaattaa tgcgtctaat aggtctaaac
     1081 gatgcaaatt tagtaattta ggattaacta aagcaatcag ctcatcattt ttaagtaccc
     1141 taaataaaac atctaacaat aactgccaag catgactact taaatcatct atatttaaaa
     1201 aatcactaaa ttgctgacaa attgccgtat tacgttgcaa aaccactaaa gcatctaaat
     1261 tttttggaaa aggacgactg acagccaccg cttccgcata acgaggcatt ggtggtgtta
     1321 atttatattg ctctttagct ttttcccacc aacgacaagc ctcatccatg acattggcta
     1381 aataggcgac ctcagcaaaa atggcagggt gaatcactgc tgaaaaataa gcatgactat
     1441 caagcagctt tagttggtta tagagttttt tccaatgtct gtcgatttta tcaagtggca
     1501 actgtatcaa aatttcagtg agtaaattat gccatgcttt tttatcatag gtattagatg
     1561 ataaaggtag ttcatggtta accagtttgc gtatgatatt gacataagaa gcaatagcac
     1621 tagtcttttt aatataaagc tctagcattt cttcgcgtaa tttattttct atgttttcgg
     1681 ggcgccacga ctgttccgtt gttacatcat ataatttttg ccagtcttgc actcgccaaa
     1741 aacattcaaa cgcacgttca taatgtgcgg ttttttcaaa caatataccc gcttcataat
     1801 aatcttctgc aatatactgc acttcagcca aacaatattg ttgttggtta taatcaccca
     1861 actgctcata caaacccgca gcttgcctta acaaataagg gtcttgatta gcaatacctt
     1921 cttgataaat ttttgtagca ttttcagtca ttaacaaaat ttgattagca tcaggcgtaa
     1981 tatctgccaa taccgctgcg cgtaggcttc ctaaatgctc aacccacgtt tcttgcgcat
     2041 ttggtaagtc cattagttgt aatggaaatg ataatggttg ctgaaaatgt tgccaaaatt
     2101 gctgattatt tgtgggggaa tcaataatta aaagctgact acgcgcacga gtcactgcaa
     2161 catacaaacg atttaaaaag tattcttgct gaattttttg ttcagcacta atcaatgctt
     2221 tacccgataa atctaaatcg actggacaat ccgcagcaaa gccataaata acaacacgct
     2281 gaaactccaa acctttagca cgaataacac tcatcgcagg aattgaagta cttccatctt
     2341 cgcgcaaatg caaatattgc ttcaaaatag gattattttg aatataactt gtttcttgac
     2401 catccaaaca gggtaaaaca atcaccaaat catattgttg agccaattgc acggtgagcg
     2461 tgtgatcttg tgtgtctatt aatatcacat ccctctgatt ggtagatgtc tgccaagact
     2521 gttgaggcac aagtgattta agaccaaaaa gttttgctct taaaccatga ataatattac
     2581 taaattgagc aatggcaggt gtggagcgaa aattataact tagctctcga taattaatat
     2641 ctgatagtgt tgcgtgttgt gcagggtcaa gggcaaaaat aaattgttct accacttgtg
     2701 ctttaattgt ttcccaacga aaccccgttg gattcagtgt ttggaattga tcacctgcaa
     2761 aaaccaatgg cacacgttgt atttgattag ggtacagatg acgcgccgaa aacaagttta
     2821 gccgcaaaac aatttgcact tcgatacgag taaaatcctg tgcctcatca caaaaaactg
     2881 cggcaaaacg tggcttaaca taatattgtt caagaacaaa acgtgctaag tcttggtcat
     2941 cccaatagcc ttctttttta cacaaaggct gataccattt ttcccatacc ttttcataaa
     3001 tagtttggta tagctcatca ctaacagaac gctgatcttc tgccaaatca agatagtcgc
     3061 taggttctaa ataataatcg gcatttaagc ctttaatata cgaacgaata atatgccaac
     3121 ataaacttgc aggatactct tgcgttgctt gtggttgttg gccaaatttt tgctcccaca
     3181 aacgcctaaa tcgaccataa tccaaccatt tttcacgacc aaaaaggctc tgctgttctg
     3241 taggcatcaa ctttagcaaa aagcctctaa actctaagca aaccgttttg ataataggta
     3301 gccatgcttg ttgcggatta catgccaata aaatttgatt aattgtctcg gcggcacggt
     3361 ccgtcagtgt ttgattacaa gtaaaatata caggataaat tggtgacttt gatgaacata
     3421 tttcttgata agcaaaatta agatacccac taaataaata ctgcaaaatt gttgatttac
     3481 cactccctgc acggccatta ataaataatg ggaatggccg ttcttcttgt agtgtaaagt
     3541 gtaaaatatg tgattcttcg ggagaaaatg ccaaattagc ggcaaaatta tgttgtacac
     3601 ttaaccattg attaggaatt tttaataacg attgtgggta aacacggcga gccgcacaca
     3661 ctaaaaactt ttgaaaatca tgaatattta atgtctctcc tttttgcatt aaatcttgaa
     3721 ttgtaaatac caattccttt gtgttaaatg tgagcaaaca cacatgatgt tgcacaaaag
     3781 gcaaaatcca agcctctccc atctctaatt caagcaacaa acaggccgtt ttttgatccc
     3841 atgctgccaa ttgttgacta atttcttcta attgttgagg tgagcataat tttagtcctt
     3901 gttccaccca cgaccacgat tcatatacca tgtctttaat attgccatac tcatgatgat
     3961 ttgtagacga aggtacggca tataaaaata aatcttcctc ctcagtgggt ttagcaaggt
     4021 tcggtaacat tggctgttgg caacgctgtt ttaaccatgt tattagcaat tgaggatctg
     4081 tcacttgctg atataaaaca tcacctaaat cgcgtatttg caaatacagc ttatggtatt
     4141 ctttatcacc acgctgaaaa atatctaaac acactaaaac atcatgtgtt tcaccctcta
     4201 tttcatggga ctccaaacga caaaccaagc gtgtgtgacg attacctaaa cgctttttaa
     4261 cccaaaaggg atgaggaaaa gcatcaagcc gccaaaatgc ctgttctcgc tcaatatcgg
     4321 caactaattg atctaatgca tctgttaatt ggtgattttt tgcacgttgt tggcactttt
     4381 cagtaatata aacaaaaata gccatgataa aaatatctca aaataaataa taacttataa
     4441 aatcttctaa gtgtttcgca gtgaattttg caaataatgc cgaaaaaata tgtggccgcc
     4501 ctaaatacat gcgtacttaa agatggctat tattcagagg attaaaaagg tagctcagag
     4561 cctcaacgat ggttgattgt tgatgactat ataaaacatt aatgcacaat acagaggatg
     4621 cagatattta ttccttaact tgttagcttt taagcaaaaa tcatccataa acatgatcat
     4681 gaccatgacc taagtaggag ttgttaatgc ggtgctcatt ttaagtatcg ttgttagtat
     4741 aatgttcgta aatagctttt attgatcaga ttgttcagtt tatgtaaata tccaagatac
     4801 tgttaatcc
//
LOCUS       DAOQMB010000157         4790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8352_length_4790_cov_17.118268, whole genome shotgun sequence.
ACCESSION   DAOQMB010000157 DAOQMB010000000
VERSION     DAOQMB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8352_length_4790_cov_17.118268"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLY05_06460"
     CDS             <1..594
                     /locus_tag="PLY05_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC02983.1"
                     /translation="TVGNYLMPPLIASFMGEHPQCQLSLEIANTSQIIQALEQFELDI
                     GFIEGFCHAPNLEVIPWQSDELVIFTAPSHPLAQKQLTQPLTEADFSEAQWILREAGS
                     GTREIFDNAVVGRLPHIHVLLELGHTEAIQRVVASGLGVGCASRLSLDEAIQNNTLQI
                     LQTPFLNLNRQLFVLIHQQKYRTYGVESFIKFATQTI"
     gene            758..2551
                     /locus_tag="PLY05_06465"
     CDS             758..2551
                     /locus_tag="PLY05_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02984.1"
                     /translation="MTTINQQLAEGLPKRDLSQLSLVSHSKNDMVKWVQALPIMNVGE
                     TTKQLYQTLQELTRVKMSEDTRFELLEELRPAVHNVLHALAKHYLNQSVLLPERANRV
                     ASLAQSLRHNLALGYKIVAYDCAEKLMTKLSIIGVGRRQQLQLAAQSFQRAISELNGL
                     LLETQLLYLSAPTGLWSDLHLLHHAANAHNVANTKVTDNHLQFEQDTTAHDSYLRAII
                     LSASQTNKLRQTEIKQIYDVSELWVTYLKLKNKFSVSDLLLIDIESDLAPTYVSKSTD
                     IPHAIYVDASKFIQHLQKIQSTPANQLHHGEQINPSLLQHLIGAWSAPSERTFPRRSY
                     EGQLLVCLGLTSAHYHFSQETEFDDFLNLPLDATEEEKNLFQRKGLGDADIGWDFHLG
                     NVPTISEEKKAQLTKGKSQKGLYPPHVTSIVNISPGGYCIRWDGDPPISLRTGEILAM
                     REPNDKTWTIGVIRWVKQLPTLGAEAGIEIISGSAIPCGARVLKKTGERTEYMRTFLI
                     KEVPSMARPATLITPNVTFRTGYNVMLRQSHDDYKVQLTTELFSTQSFSQFTFAWLQA
                     PTKPQNTANQQNSNTNTVEAKDDFDTLWGNL"
     gene            2658..4742
                     /locus_tag="PLY05_06470"
     CDS             2658..4742
                     /locus_tag="PLY05_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC02985.1"
                     /translation="MATKSETLRLLVLHSSADNTEDIIKSLKGAGTPTRPQLIATLED
                     FKELLKNQVWDMVLTAENLSDGAYNDVVQVIRDSDKDVPVIVLLNEYNEDIIVEALEM
                     GAVDAIVYNQHQHIVFCIRRELTNLYHRRAKRAAESFLKETEKRCQLLLENSRDAIAY
                     VHDGMHIYANKAYVELFGYESGEDLEGMPMVDLVGKQDLANFKDYLRAYSKGEKISND
                     LKFHGVKADGDQIDAMMQLSTASYDGERCTQIIIRRDDSAGQATLLAEQLKAATSIDQ
                     LTGLPNRQQMEEKLEETLGIARRDKTQSALLYMGIDNLPYITTTAGIVGADNVIVELS
                     KALANLMPNAILARFSDSVFAAIIQDTDSEKAKQTAEKTCQAIYERLFDIPNGKTIQT
                     TLSIGVAMIGETAPDIPELLSRAVNASEKVKLQNKGTGNGVSVYNPAENAAANDSALR
                     ELLVEALEQSKFKLLFEPLFDVEDETAQFYEVFVRLPLADGKVMAPDEFLDVAQRYQL
                     GTKIDRWVLINACKRLKEHLFRHPGSRLLLNLTAESLQDASLPELITKLTKAIDPKGS
                     PLVVQFSENDVVTYLKQAKEHSSLLKANGIDVSITNFGCTLNPLNTLKHVEAEYIKLD
                     RSFTQDLTQEANLDNTKKFASELNSLNKRVIVSYVENAATVSKLWTMGVRYLQGYYLQ
                     PPSENMVYESGE"
BASE COUNT         1544 a          924 c          868 g         1454 t
ORIGIN      
        1 acggtgggta attatttaat gccaccatta attgccagtt ttatgggtga acatccacaa
       61 tgtcaattaa gcctagaaat tgccaatacc agtcaaatta ttcaagcttt agaacaattt
      121 gaactggata ttggttttat tgaaggcttt tgtcacgcgc ctaatttaga agtcattcct
      181 tggcaatctg acgaattagt catatttact gcaccttctc acccactcgc ccaaaaacaa
      241 ttaactcagc cgcttaccga agccgatttt agtgaagcgc aatggatttt gcgtgaagct
      301 ggctctggta cacgcgagat ttttgataat gcggttgttg gtcgcttgcc acacattcat
      361 gttttattag aattaggcca taccgaagcc attcaacgag tcgtagccag tggtttgggt
      421 gtgggctgtg cttcacgact ttctttagat gaagcgatac aaaacaatac cttacaaatt
      481 ttacaaacac cctttttaaa tctaaatcgc caactttttg tgctaattca tcaacaaaaa
      541 taccgaacct atggcgtaga aagctttatt aaatttgcca ctcaaacaat ttaaccgtca
      601 ataatcttct tataagatat ttgcattact tatggttagc gtataatctt cgcttagtat
      661 aaaattcaaa gcgatacaca caaaaatacc attacgccac tattaacccc tattgtggta
      721 gtgagccaaa tggactcagc atcaggccga aaacactatg acaacaatta atcaacaact
      781 agcagaaggt ttacctaaac gcgatttatc tcaattaagt ttagtaagtc attctaaaaa
      841 tgatatggtt aaatgggttc aagccttgcc tattatgaat gtaggcgaaa caacaaaaca
      901 gttgtatcaa actctacaag aattaacacg tgtcaaaatg tctgaagaca cacgctttga
      961 actcttagaa gaattacgtc ctgctgtgca taatgtttta cacgccttag ccaaacatta
     1021 tctcaatcaa tccgttctat tacctgagcg tgccaaccgt gttgcttcat tagcgcaatc
     1081 tttacgtcat aatttagcat tgggctacaa aattgttgcc tatgattgtg ccgaaaaact
     1141 aatgacaaag ttaagcatta ttggtgtagg tcgtcgtcaa caattacagc ttgctgcaca
     1201 aagctttcaa cgtgccatca gtgaacttaa cggtttatta cttgaaacac aattattgta
     1261 tttatctgcc cccacaggat tatggtcaga tttacattta ttacatcatg ctgctaatgc
     1321 acataacgta gctaatacca aggtcacaga caatcactta caatttgaac aagatacgac
     1381 agcgcatgat agttatttac gagccattat cctgtctgct agccaaacta acaaattacg
     1441 tcaaactgaa attaaacaaa tttatgatgt atcagaatta tgggtcactt atctaaaatt
     1501 aaaaaataaa ttttcagttt cggatttatt actcattgat attgaaagtg atttagcacc
     1561 aacttatgtg tctaaatcaa ccgatattcc ccatgctatt tatgtggatg cctcaaaatt
     1621 tattcaacat ttacaaaaaa ttcaaagtac gcctgcaaac caactgcatc acggggaaca
     1681 aatcaatccg agtttattac aacatttaat tggtgcttgg agtgcacctt ctgaacgtac
     1741 attcccgcga cggagttacg aaggccagtt attggtttgt ttaggtttaa catctgcaca
     1801 ctatcatttt tctcaagaaa cagagtttga tgattttttg aatttacctt tagatgctac
     1861 tgaagaagaa aaaaatctct ttcaacgcaa agggcttggt gatgccgata ttggctggga
     1921 ttttcatcta ggtaatgtcc ccactatcag cgaagagaaa aaagcacaat taaccaaagg
     1981 caaaagtcaa aaaggcttat atccacctca tgtcacttca attgtcaaca ttagtccagg
     2041 tggctactgt attcgttggg atggtgatcc tcctattagt ttacgtaccg gtgaaatttt
     2101 agccatgcgt gaacctaatg ataaaacgtg gacaattggc gttattcgct gggttaaaca
     2161 actaccaaca ttaggtgcag aagctggcat tgagattatt tctggtagtg ctattccttg
     2221 tggtgcacgt gtactcaaga aaacgggcga acgtaccgaa tatatgcgta catttttaat
     2281 taaagaagtg ccatcaatgg caagacctgc tacattaatc actcccaatg tgacgtttag
     2341 aacaggctat aatgtcatgt tacgacaaag tcatgatgat tataaagttc aattgaccac
     2401 agagttattt agcactcaaa gttttagcca atttactttt gcttggttgc aggctccaac
     2461 taagcctcaa aatacagcaa atcagcaaaa ttctaatacc aatactgttg aagctaaaga
     2521 cgattttgat acactatggg gaaatttata aattttagct tatttttgtt gcgcagacca
     2581 ctcaaaaagt ggcaactgcg cttctagcta tctttatcgc tccatatatt ttttgaatcc
     2641 tataggcaat tatttttatg gctacgaaaa gtgaaacctt acgtctgcta gtattgcatt
     2701 cgtcagccga caataccgaa gatattatta aaagtttaaa aggtgcaggc actcctactc
     2761 gcccacaatt aattgcgaca ttagaagact ttaaggagct tctcaaaaat caagtatggg
     2821 atatggtttt gacagccgaa aacctcagtg atggtgctta taatgatgtc gtacaagtta
     2881 ttagagattc cgataaagac gtaccagtca ttgttttatt aaatgaatat aacgaagata
     2941 ttattgtcga agctttagaa atgggtgctg ttgacgccat tgtctataat cagcatcagc
     3001 atattgtttt ttgtattcgt cgagaattaa ccaatcttta tcatcgtcgt gctaaacgtg
     3061 ccgcagaatc atttttaaaa gaaaccgaaa aacgttgcca attgctactt gaaaactcac
     3121 gtgatgctat tgcttatgta catgatggta tgcacattta tgccaataaa gcatacgtag
     3181 aactatttgg ctatgaatct ggtgaagacc tagaaggaat gccaatggtt gacttagttg
     3241 gcaaacaaga cttagctaac tttaaagatt atttacgagc ttatagcaaa ggcgaaaaaa
     3301 tatctaatga tttgaagttt catggtgtca aagccgatgg ggatcaaatt gatgccatga
     3361 tgcaactttc tacagcaagt tatgatggcg aacgttgtac tcaaattatt attcgtcgtg
     3421 atgatagtgc tggccaagct acccttcttg ctgaacaatt aaaagcagct acctctattg
     3481 accaattaac aggcttacca aatcgtcaac agatggaaga aaaactagaa gaaactttag
     3541 ggattgctcg ccgtgataaa acccaaagtg ccttgctcta tatgggtatt gataatttac
     3601 catacattac aacaacggct ggtattgttg gagccgataa cgttattgtt gaattatcca
     3661 aagctttagc taatttaatg ccaaatgcca ttctagcgcg ctttagtgat agtgtatttg
     3721 ctgcaattat tcaagataca gactcagaaa aagctaaaca aactgccgaa aaaacctgcc
     3781 aagctattta cgaacgttta tttgatattc ctaatggcaa aaccattcaa acaacattga
     3841 gtattggtgt tgctatgatt ggtgaaaccg cacctgatat tccagaatta ttatctcgtg
     3901 cagtcaatgc gagcgaaaaa gttaaactac aaaataaagg gactggtaac ggcgtcagcg
     3961 tttacaatcc agccgaaaat gctgctgcta acgactcagc tttacgtgaa ttattagttg
     4021 aagccttaga gcaaagtaaa tttaaactat tatttgaacc tttatttgat gttgaagacg
     4081 aaacagctca gttttatgaa gtctttgtac gcctgccact cgccgatggc aaagtcatgg
     4141 cacctgatga atttttagat gtcgctcagc gttatcaatt aggcactaag attgatcgtt
     4201 gggtattaat caatgcttgt aaacgattaa aagagcattt atttcgtcac ccaggtagcc
     4261 gcttattact taatttaacc gctgagtctt tgcaagatgc gagcttgcca gagctaatta
     4321 ctaaactaac taaagcgatt gatcctaaag gtagtccact tgtagtacaa ttctctgaaa
     4381 atgacgttgt gacctacctc aaacaagcaa aagaacatag ctctttgtta aaggcgaatg
     4441 gtattgatgt ttctattacc aactttggtt gtacacttaa ccctttaaat acccttaaac
     4501 acgttgaagc agaatacatc aaacttgacc gttcttttac ccaagattta acccaagaag
     4561 ctaacttaga taatacgaaa aagtttgcca gtgaattaaa ttcattaaac aagcgagtga
     4621 ttgtttctta tgttgaaaat gctgccactg tttccaaact ttggacaatg ggagtacgtt
     4681 atctacaagg ttattattta caacctccct cagaaaacat ggtttacgag agcggtgaat
     4741 aaacaagggg caatgcccct tgtttcattt taggcaacat agtttatctt
//
LOCUS       DAOQMB010000158         4778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8382_length_4778_cov_5.301927, whole genome shotgun sequence.
ACCESSION   DAOQMB010000158 DAOQMB010000000
VERSION     DAOQMB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8382_length_4778_cov_5.301927"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..868)
                     /locus_tag="PLY05_06475"
     CDS             complement(50..868)
                     /locus_tag="PLY05_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02986.1"
                     /translation="MLKKVLLLSSLTLLAACNETDSNDISTDKLVLNGSLFVENDAAK
                     MIVDFTKEKSHVTVVLNGGDFVQATDSKSTNVMEYQDDTIWGSSYRATLPLDINNSYK
                     VIFNRAAQNQKFESTFPAIPAAFSIVAPIDKQTFSVTSNPTIDVKWDNKIPSDKLFLR
                     GRYSCGWTANPAVLDAKSQKMLSELTNGQAIDITLNDKERDLKTRILHVQEAVQSMTE
                     GLKNSYPDSTLTFNGCDVDLELIAKSFSTASAEFSGKSELQSYRKVTMETTLTP"
     gene            complement(928..2124)
                     /gene="purT"
                     /locus_tag="PLY05_06480"
     CDS             complement(928..2124)
                     /gene="purT"
                     /locus_tag="PLY05_06480"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013198146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HNC02987.1"
                     /translation="MKFGTPLSSNAIKVMLLGSGELGKEVVISLQRLGVEVIAVDRYA
                     NAPAHQVAHRAHIINMSNADELRALILAEKPHFIVPEIEAIATQVLVDIEQQGIAEVI
                     PTARAVNLTMNREGIRRLAAEQLQLPTSPYAFAESLEQLQAAAEKIGFPCFIKPVMSS
                     SGKGQSKLDSMDELAAAWDYALASGRVNHGKVILEGMINFDFEITLLTVRAVGASGQV
                     QTFFCDAIGHKQVAGDYVESWQPQAMSEVALAKARDIADKVTTNLGGRGIFGVELFVK
                     GDDVWFSEVSPRPHDTGLVTLASQYQSEFEIHARAILGLPVNTACHSVAASAVIYGGL
                     EAQGIAFEGVAEALKVPNSDLRLFGKPESFTKRRMGVALCRADNVDLARANAKESAGR
                     VKPILA"
     gene            2202..3113
                     /locus_tag="PLY05_06485"
     CDS             2202..3113
                     /locus_tag="PLY05_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNC02988.1"
                     /translation="MIIRQRQHWLRMLFIWRGSVLLRILPQLAAVTGLAILVTVLHGR
                     VFELKVPLTFVPFTLIGVALAVFLGFRNNASYDRYWEGRKLWGSLLSESRTLARQYLT
                     LVTDTQQQQTFVYSLAALSHALRHQLRGTEASHDLQRLLSVDVYQTLKNARFIPNLLI
                     LHLAQQLQKQRLQQNLEPILAAQMELPMAKLTENICGCERLSSTPLPYTYVVILHRTA
                     YLYCFLLPFGLVDAIGWTTPIMVAFIAYTFFALEALSDELEEPFGTSPNDLALEAMCV
                     MIETTLKEMLGEKDLPEAVRAVDFVVL"
     gene            3151..>4778
                     /locus_tag="PLY05_06490"
     CDS             3151..>4778
                     /locus_tag="PLY05_06490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HNC02989.1"
                     /translation="MNYQKQHLGGLDGLRAVAVLAVILYHLNLSNFFSGGFLGVDIFF
                     TLSGFLITSLLLKEYEKTQTIDLKIFYVRRMKRLLPAMLGVIVICSALVPFVAQDAVF
                     QLRKDIPAALLYYSNWWQIVSEQSYFEMMNRPPLLQHLWSLAIEEQFYVFWPLLVLII
                     VRYGHARWLLWFASSLMLISAACMGFLAIHNDIPLEADPNRLYLGTDTHTSGLFAGAM
                     LAYLWNPWRINFQSQKAYLPVVFSLLGVCGLLVLCALFTWANETQAWLYRGGFLGVAF
                     ATVFVIIGATYRGGYLEKALSIPIMRYLGERSYGLYLWHWPIFVLIRPQDFIINEWLT
                     QGLRLIVTLFAAELSYRYIETPLRFDHIHTLQRPKQWQWLGISAASIMAVVVLYQQPV
                     PMFTTTTEIISHEPVIMTTPNALASPAKIEVITPPHMVTGPIELTHMLAIGDSVMLGA
                     ADYLKRGVAGISVDAAVGRQGRDAIKLIRQLKADGKLPARLLIHMGTNGYLPVSQFKQ
                     LITDLSDREEIVLINIQANRRWMGDNNDLLNTFKT"
BASE COUNT         1310 a          930 c         1030 g         1508 t
ORIGIN      
        1 ccgcacggga ggctttttcc tcccctgagg atggacttaa aaaagtcgct tagggggtta
       61 atgtggtttc catagtcact tttcgatagc tttgtaattc acttttgccc gaaaactcag
      121 cacttgctgt actaaaactt ttagctatga gctctaaatc tacatcacaa ccattaaagg
      181 tcaatgtgct atcaggataa gaattcttta agccttcggt catagattga acagcttctt
      241 gaacatgtaa aatacgtgtc tttaaatctc tttctttatc atttaaagta atatcaatag
      301 cttgtccatt agttaattcg ctaagcattt tttgagattt ggcatctaac acggcaggat
      361 ttgcagtcca gccacagcta tatctgcctc gcaaaaaaag tttgtcgcta gggattttgt
      421 tgtcccattt cacatcaatg gttgggttag atgttacgga aaaagtctgt ttgtcaatag
      481 gagcgacaat actaaatgcg gcaggaatgg cagggaatgt actttcaaat ttttgatttt
      541 gtgctgcacg attaaaaata actttatagc tattattgat atctaaggga agtgttgcac
      601 gataagagct accccaaata gtatcatctt gatattccat gacattagta cttttgctat
      661 ccgttgcttg tacaaaatca ccaccattta gtacaacagt gacatgagat ttttctttag
      721 taaaatctac tatcatttta gcagcatcat tttctacaaa gagactacca ttgaggacta
      781 atttatctgt actaatatca ttagaatcgg tttcgttaca ggctgctagt agagttaagc
      841 tactaagtag taatactttt tttagcatga tatttccttt ctgtaagaca aaaatgattg
      901 gcttttgtct tctgaattgt ctaaaagtta agctaaaata ggctttactc gtccagcact
      961 ttctttggca tttgctcttg ctaagtcaac attatctgca cgacataacg ccacacccat
     1021 acgacgttta gtaaaacttt cgggtttgcc aaataaacgc aaatcgctat tgggtacttt
     1081 tagtgcttcg gcaacgcctt caaaagcaat gccttgcgct tctaaaccgc cataaattac
     1141 cgcactagcg gccacactat gacaagcggt attcacaggc aaacctaaaa tcgcacgagc
     1201 atgaatttca aattctgatt gatattgact agccaaagtg actaaacccg tatcgtgtgg
     1261 gcgggggcta acttcggaaa accatacatc atcacctttg acaaataatt caacgccaaa
     1321 aatgccacga ccgcctaaat tggtagtgac tttatcagca atgtcacggg cttttgctaa
     1381 ggcaacttca ctcatggctt ggggttgcca actttcgaca taatcccctg ccacttgttt
     1441 gtgaccaatc gcatcacaga agaaagtttg tacttggcca cttgcaccca cagcacgcac
     1501 agtgagcagg gtaatttcaa aatcaaaatt aatcatacct tcaagaatca ctttgccatg
     1561 attaacacga ccactggcta aggcgtaatc ccatgccgcc gctaattcgt ccatgctatc
     1621 taatttagat tgacctttac ccgaagagga catcacaggt ttgataaaac atggaaaacc
     1681 gattttctcg gcagccgctt gcagttgttc taaactttcg gcaaaagcat aaggagacgt
     1741 gggtaattgt agttgttcgg cagccaagcg gcgaatgcct tcgcggttca tggttaagtt
     1801 cacggcgcgt gcggtgggaa tgacttcggc aatgccttgt tgttcaatat caactaaaac
     1861 ttgggtggcg atggcttcaa tttcggggac gataaaatgc ggtttttcgg ccaaaattaa
     1921 tgcccgtaat tcgtctgcgt tactcatgtt gataatgtgg gcgcgatgcg ccacttgatg
     1981 ggcaggggca ttggcataac ggtcaacggc tatgacttcg acccctaaac gctgtaaaga
     2041 aattaccact tctttaccca attcgcccga acctaataac atgactttaa ttgcgttact
     2101 gcttagggga gtgccaaatt tcataacgga ttactctaaa agcccaaaat tgctgcgtat
     2161 aatataagac ttaggtatga cacggctatt ggaattatta tatgattatt cgtcagcgtc
     2221 agcattggtt acgaatgttg tttatttggc gtggttcggt gctgctgcga attttgccac
     2281 aattggcggc ggtgacaggt ttggctattt tagtcacggt gttacatggt cgcgtttttg
     2341 agttaaaagt gcctttaacc tttgtgccgt ttaccttaat tggggtagct ttggcggtgt
     2401 ttttgggctt tagaaataat gccagttatg accgttattg ggagggacgc aagttatggg
     2461 gcagtctgtt aagtgaaagc cgaactttag ctcgtcaata tctcacttta gtgacggata
     2521 ctcagcagca acaaactttt gtctatagtt tggcggcttt aagccatgct ttgcgtcatc
     2581 aattgcgtgg tacagaggca agccatgatt tacaacgatt attaagcgta gatgtttatc
     2641 aaacactcaa aaatgcgcgt tttatcccca atttattgat tttacacctc gctcaacagc
     2701 tacaaaagca acgcttacaa cagaatcttg agccaatttt agcggcacaa atggaactgc
     2761 caatggcaaa actgaccgaa aatatttgtg gttgtgagcg tttgagtagt acgcctttgc
     2821 cctataccta tgtggtgatt ttgcaccgta ccgcatattt atattgtttt ttattgcctt
     2881 ttggtttggt ggacgcgatt ggttggacaa ctcccattat ggtggcgttt attgcgtata
     2941 cgttttttgc cttagaagcc ttaagtgatg aattagaaga gccgtttggc actagcccca
     3001 atgatttagc cttagaagcc atgtgtgtga tgattgaaac gacactaaaa gaaatgttgg
     3061 gcgaaaaaga cttgcctgaa gcagtgagag cggtggattt tgtggtattg tgaagaagag
     3121 tattatagtg ctaactaagg taaggtacgt atgaattatc aaaaacaaca tttaggtggc
     3181 ttagatggat tacgtgctgt cgcagtttta gctgttattt tgtatcacct aaatctaagt
     3241 aatttttttt caggaggttt tttaggcgtt gatatttttt ttacgctttc tggatttttg
     3301 attactagct tattattaaa agaatacgaa aaaactcaaa ctattgattt aaaaattttt
     3361 tatgtccgtc gtatgaaacg cttattgcca gctatgttgg gcgttatcgt gatttgcagt
     3421 gccctagtgc cgtttgtggc gcaagatgct gtttttcagc ttcgtaagga tattcctgcg
     3481 gccttattgt attactctaa ctggtggcaa attgtaagtg agcaatcgta ttttgaaatg
     3541 atgaatcgcc cgccattgtt gcagcatttg tggtctttgg cgatagaaga gcaattttat
     3601 gtcttttggc ccttgcttgt attaatcata gtacgttatg gccatgctag atggttatta
     3661 tggtttgcca gttctttaat gcttatctcc gccgcctgta tgggcttttt ggccatacac
     3721 aacgacattc ctcttgaggc cgaccctaat cgattatatt tggggacgga tactcatacg
     3781 agcggtttgt ttgcgggggc aatgctagct tatttatgga atccctggcg catcaacttt
     3841 cagtcacaaa aagcttatct gccagtcgtt tttagtctgc ttggcgtatg tggcttgtta
     3901 gtattgtgtg cgttgtttac ttgggcaaat gaaacacaag catggttata tcgaggtgga
     3961 tttttaggtg tggcatttgc cacagtgttt gtcattatag gtgcaacgta tcgaggaggg
     4021 tacttagaaa aagcgttaag cattcccatt atgcgttatt tgggagaacg ttcttatggc
     4081 ttatatttgt ggcattggcc aatatttgta ttaattcgcc cacaagattt tatcattaac
     4141 gaatggctta cgcagggatt acgccttatt gtcacgttat tcgcagctga actctcttat
     4201 cgttacatag aaacgcctct acgctttgat catatccata cattgcaacg ccctaaacaa
     4261 tggcaatggt taggtatttc agcggcaagt attatggctg tcgttgtttt gtatcagcag
     4321 ccagttccca tgtttacgac cactacggaa ataataagcc atgagccagt tattatgaca
     4381 acaccaaacg cattagcatc gccagctaaa attgaggtga taacgccacc gcatatggta
     4441 actggtccga ttgaactaac tcatatgttg gcgattggtg attcggtaat gttaggggcc
     4501 gcagattatt taaaacgtgg agtagcaggt atcagtgtag atgcagcagt cgggcgacaa
     4561 ggacgtgatg ccattaaact gattcgccaa ttaaaagcag acggaaagct gcctgcacga
     4621 ttattaattc atatgggaac caatggttat ttgccagtct cacaatttaa acaattgata
     4681 actgatttat cagacagaga agaaattgtg ttaattaaca tacaggcaaa tcggcgttgg
     4741 atgggagata acaacgattt attaaatacc tttaaaac
//
LOCUS       DAOQMB010000159         4725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8527_length_4725_cov_7.392934, whole genome shotgun sequence.
ACCESSION   DAOQMB010000159 DAOQMB010000000
VERSION     DAOQMB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8527_length_4725_cov_7.392934"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2349
                     /locus_tag="PLY05_06495"
     CDS             127..2349
                     /locus_tag="PLY05_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02990.1"
                     /translation="MKLPLKTLCLAIAIVNMQSAFALESLDDETMSETTGEGIALLPE
                     NYSFMMNGANPSTGLYTDNSGAGTYGTGYMRFIPVGPLTTTATNNGYQKADAWLYGVT
                     LGQSKKAYGATIDSTDWGVPFGAISATPAATDFGRTITSWGTAENPWVVKTVTDNTVP
                     NFTGTTKSVTYLGLEAPLYNTAAISSLSASEQSAYNLKLGYWMDVFMRNAAVADSGTT
                     AELSNRLRLAFAWNGFSMNGSNFKIFQTLDGATAGGTYSATLKINGTDTLKTFNKGLS
                     TSYNQTFGMAGLLRFNSVPTDARRGTVSNQTNTRQIIRLDYNPLSNYDVNSNTRSGNY
                     FENGILQTTSGSIASAAAEGTNTTAGITETYQPMTETSLRGPAGQTPPYQASYPGTFP
                     NGGVCTDPDAIPNVGSGNDQYGQCVSNEGYTTRRFKAFGYNDWAPPSERSVMRISTRE
                     LGTAFAGTPALGGATGGVPNFQPGATAEGLFFYDVNINLVLGNLYQPIMLSTDGNNFS
                     FEMARIPNVSSIYTKIYTRYPGDLGDPGVTYEGSTCNIYQCGTHPVGTNTTISGNYQA
                     STATHSSITIGATERVNSKVVNSITYNNVNQIEAHKGVGAYGISIGELKSGSGIYSES
                     VVDYTQVWLRTRAHNYCGAFCGSSFGAWGAWQAQSPKTATTSTLHPSLDRPEPYETVQ
                     RQNYNNQILGIQTTMPVGTMASTLNAMGSPGGSVNNNFGSAVIDGLLIQHLKFSTTGL
                     "
     gene            2363..3043
                     /locus_tag="PLY05_06500"
     CDS             2363..3043
                     /locus_tag="PLY05_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02991.1"
                     /translation="MNRLTKIISSLICAMLIVSPSYAMDNLADEDLAEVSGQAGADLS
                     VKFTINHDANANFVCTDLVYCRLGMSLSNRYDDGSLDTYDINGNRTPSTTGRKQWLVF
                     KGIQGTINIQELKFDGEDVVYGSTTKASLKLGFTSTKPIELRNFGFSSLSIETDACAE
                     GNVACSMTATAGNTPGYLTPATLYGGVGFDATKEKGFLGLNINTNLSLTGSVKMFSCS
                     SANNHPRC"
     gene            3067..>4725
                     /locus_tag="PLY05_06505"
     CDS             3067..>4725
                     /locus_tag="PLY05_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02992.1"
                     /translation="MKKTIKKTRQFSLTILSLSILLAQSCYALQVLEDNDLRDVEGQD
                     GLDVNISYSEADIGNMYWEDSVGTATTGDPEQALRASANTVKIRDLKPADGLTLGGRY
                     QVDIGSNGTRTGLSLKTTLNPFTLSVDSFSICDLSAVCDPSGASGAYNGTVGSLAVQV
                     DSPMQIDITTPDGLFNETEQASLSLGVQNLNVYMGLKTAPAATTQNQLILKNFNFNFG
                     GKGVFYVDETKGLMLETSSGIASGAPKASKTQAPNTTHGYIDFTRVTDPDQTGAATGT
                     YGGTSAGLNLEFLTKKETAPSANTYSLADTKGLIRLGASGRMVNSYLQLRGISMNGVT
                     APTDNNVTNTHILNNVLGYASNNSGTGSGVTNTVMGSKGVNLRFRGEFTTAGDAMLGG
                     DDTKETKLEIGGAGQNAFGFEFSKLQPLAFGSTERAYFDSGDIYFNLANTRHLRMPEN
                     SVLMTSRFGGNTTTFLTSSADYVQQVHNFPDNPYSFVSAIRGSEFQAISRRGRFTSSS
                     NVSAGNAIAASDGTANEWGLGLPFYNLNANIAMYSSNYTGDVYTL"
BASE COUNT         1454 a          814 c         1033 g         1424 t
ORIGIN      
        1 aataccatgt aactgctgct tagctttggc aaagtggcaa aaaaatagca taatactaaa
       61 gagatttaat aattctgtac ttatttattc aaaaataaga cttacaacaa taaatagggc
      121 ttagctatga agttgcctct taaaaccctt tgtttggcta ttgctattgt aaatatgcaa
      181 tctgcttttg ctttagaatc gttagatgac gaaactatga gtgaaacaac tggtgaaggt
      241 attgctttat tgccagaaaa ttactctttt atgatgaatg gagctaatcc gtcaactggt
      301 ctttatactg acaatagtgg tgctggtact tatggtacag gctatatgcg ttttattcct
      361 gttggtcctt taactaccac tgcaacaaat aatggttatc aaaaggctga tgcttggttg
      421 tatggcgtaa ctttaggaca atctaaaaag gcgtatggag cgactataga cagtacggat
      481 tggggagttc cttttggtgc gataagtgct acgcctgccg cgacagactt tggtcgcaca
      541 attacatcgt ggggaacagc cgaaaatcct tgggttgtca aaacggttac tgataacact
      601 gtaccgaatt ttacgggtac aactaagtca gtaacatatt tgggattgga agccccttta
      661 tataatactg ccgctattag cagtttgtct gcctcagaac agtccgctta taacctaaaa
      721 ttaggctatt ggatggatgt gtttatgcgt aatgctgccg ttgcagatag tggaacaaca
      781 gcagaattaa gtaaccgttt gcgtttggct tttgcatgga acggttttag tatgaacggt
      841 tctaatttta aaatctttca aactttagat ggagcaactg caggcggcac atacagtgca
      901 acacttaaaa tcaatggcac tgatacacta aaaaccttta ataagggttt aagtacatcg
      961 tataatcaaa catttggtat ggcaggtttg ttgcgtttta atagtgttcc aacagatgcg
     1021 aggagaggta ctgtttctaa tcaaacaaat actcgtcaaa ttatccgact cgattataat
     1081 cctctttcaa attacgatgt taatagtaat acgcgctcag gtaattattt tgaaaatggt
     1141 attctgcaaa caacatcagg atctatagcg tcggcggcag ctgaaggcac taatacgacg
     1201 gcagggataa ccgaaaccta tcagccaatg actgaaacaa gtttaagagg tccagcaggc
     1261 caaacacctc cttatcaggc atcctatccg ggtacatttc ctaatggtgg tgtttgtacc
     1321 gatcctgatg caattcctaa tgtgggcagt ggtaatgatc agtatgggca atgtgtttct
     1381 aatgagggtt atacgacgcg tagatttaag gcatttggtt ataatgattg ggctccacca
     1441 tcggaaagaa gtgtaatgag gattagtact agagaattag gaactgcatt tgcgggaacc
     1501 cccgcattgg gaggggcaac aggtggtgtt cctaattttc aaccaggtgc gaccgctgaa
     1561 gggttatttt tttatgatgt aaatattaat ctggttttag gtaatttata tcagccaatt
     1621 atgttgagta cagatggtaa taacttttct tttgaaatgg ctcgtattcc aaatgtttct
     1681 agtatctata caaagatata tactcgctat ccgggtgatt taggggatcc tggggttact
     1741 tatgaggggt caacctgtaa tatttatcaa tgtggcacgc atccagttgg tacaaatacg
     1801 acaatcagtg gtaactatca agcatctacg gcaacacata gcagtatcac tataggggca
     1861 acagaaagag ttaactcaaa agtagttaat agtattactt ataataatgt taatcaaatt
     1921 gaagctcaca aaggggttgg ggcgtatggt atctctattg gagagttaaa gtcaggtagc
     1981 ggtatttata gtgagtctgt tgttgattat acacaagtat ggcttagaac aagagctcat
     2041 aattattgtg gcgctttttg tggtagctcc tttggcgcat ggggtgcttg gcaagcacaa
     2101 agtcctaaaa ctgcaactac aagcacacta catccttctt tggatagacc tgagccctat
     2161 gagactgttc aacgccaaaa ttacaataat caaattttag gtattcaaac tacaatgcct
     2221 gtcggtacaa tggcatcaac tctaaatgcg atggggtctc caggtggttc agtgaataac
     2281 aactttggct cagcagtgat tgatggctta ttaattcaac atttaaagtt ttcgactaca
     2341 ggtttataag aggataaaaa taatgaatcg cctcacaaaa ataataagta gcctaatttg
     2401 cgcaatgttg atagtgtctc catcttatgc catggataat ctggctgatg aagatttggc
     2461 agaagtttcg gggcaagcag gtgcagattt aagtgtaaaa tttaccatta accatgatgc
     2521 taatgccaat tttgtgtgta ctgacttggt ttattgtcgt ttgggaatgt cgcttagcaa
     2581 tcgttatgat gatggcagtt tagatactta tgatataaat ggaaatcgta ctccatcaac
     2641 aactggccgc aaacaatggt tagtctttaa gggtattcaa ggcacgatta atattcaaga
     2701 gttaaaattt gatggtgagg atgttgtata tggctcaaca accaaagcat cattaaaatt
     2761 gggcttcacg tcaacaaaac caatagaact tagaaatttt ggttttagct ctttatctat
     2821 cgaaacagat gcttgtgctg aaggaaatgt tgcctgttca atgacggcaa cagcaggtaa
     2881 tacaccaggt tatttaacgc ctgctaccct atatggcgga gtagggtttg atgccaccaa
     2941 agaaaagggc tttttgggct taaacattaa taccaattta tcattaacag gtagtgtaaa
     3001 aatgttttct tgctcaagtg ccaataacca tcctcgttgt taaactgaca aaagggtaaa
     3061 tatataatga aaaaaactat aaaaaaaacg cgtcagttta gtttaacaat tttatctctc
     3121 agtattttat tggctcaatc ctgttatgcg ctacaagtct tggaagataa tgatcttaga
     3181 gatgtagaag gacaagatgg tctagatgtt aatattagct actcagaagc agatataggt
     3241 aatatgtatt gggaagattc ggtgggtacg gcaacaacgg gagaccccga gcaagcctta
     3301 agagcttcgg ctaatacggt aaaaattaga gacctgaaac ctgctgatgg attaacattg
     3361 ggagggcgtt accaagttga tattggtagt aatggtacaa gaacaggttt atcactaaaa
     3421 actacattaa atccttttac cctctcagtt gatagttttt ccatttgtga tttgagtgcg
     3481 gtatgtgatc catcaggtgc atcaggtgct tataatggta cggtaggtag tttggctgtg
     3541 caagttgact cgcctatgca aatagatatt acgactcccg atggcttgtt taatgaaacc
     3601 gagcaagcct ctttgtcctt aggcgttcaa aatctgaatg tgtatatggg cttaaaaaca
     3661 gcacctgctg caaccacaca aaaccaatta attttaaaga attttaactt taattttggt
     3721 ggcaagggcg tattttatgt tgatgagacc aaaggattaa tgttagaaac tagcagtggt
     3781 attgcttcag gcgcaccaaa agcttcaaaa acccaagcac ctaataccac acatggttat
     3841 attgatttta cacgggtcac agatcctgat caaacagggg cggcaacagg cacttacggt
     3901 ggaacgagtg caggtttaaa tttagagttt ttaaccaaaa aagaaactgc gccttccgca
     3961 aatacttata gtttggccga cacaaaaggc ttgatccgct taggtgctag cggtcgtatg
     4021 gttaatagct atttacaatt acgaggcatc agtatgaatg gggtgacagc acccactgat
     4081 aacaatgtga caaacactca tattttaaat aatgttttag gttatgccag taataatagt
     4141 ggtacaggtt ctggggttac taatacagta atgggatcta aaggtgtaaa tttaagattt
     4201 agaggagagt ttacaactgc gggtgatgca atgttagggg gcgatgacac taaagagacc
     4261 aaactagaaa taggtggtgc tgggcaaaat gcctttggct ttgaatttag taaattgcaa
     4321 cctttagcat ttggatcaac agagagagcc tattttgata gtggtgatat ttactttaac
     4381 ttagccaata cgcgtcattt gcgtatgcct gaaaactcgg ttctaatgac ctctcgattt
     4441 ggtggaaaca caacaacatt tttaacatca tccgccgatt atgtgcaaca agtacataat
     4501 tttcccgata atccttatag ttttgtttcg gcaattcgcg gctctgagtt tcaggcgatt
     4561 tctcggcgtg gtcgttttac ctcaagtagt aatgtatcgg caggtaatgc tattgcagca
     4621 tcagatggaa cagcaaatga atggggtcta ggtttaccat tttataattt aaatgccaat
     4681 attgccatgt atagcagcaa ttatacaggt gatgtatata ccttg
//
LOCUS       DAOQMB010000160         4723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8542_length_4723_cov_4.196658, whole genome shotgun sequence.
ACCESSION   DAOQMB010000160 DAOQMB010000000
VERSION     DAOQMB010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8542_length_4723_cov_4.196658"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..>4723
                     /locus_tag="PLY05_06510"
     CDS             112..>4723
                     /locus_tag="PLY05_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC02993.1"
                     /translation="MYNVFDFRNNLIKDYSSFSRSFTRIAAVDILSEVNSQYDNGRYW
                     PEPLIQINPNYQRKGTVQQLAQEGVLHKECADIFQVEKLSHIPQPLHLYSHQLEAIAK
                     AKAKQSFIVTTGTGSGKSLSFFIPIIDQILKAKDLDKTPRTRAIIIYPMNALANSQLE
                     ELNKFLYGYQPEQQPFTVARYTGQENNIERESIAKNPPDILLTNFMMLELILTRFEET
                     DRHVVANCQGLDFLVLDELHTYRGRQGADVAMLVRRLRERLQAKNLICIGTSATMSST
                     GTVEDQKNTVAGVASKLFATTITANDVIGETLERVTDTNRNVDAIRLELAHSVNRKDF
                     YWPSFTAFQHDPLAIWVELTLGVELPKEGRPRRAKPKTVSEAASQLAKDAACSEQQAK
                     EVLQCFLVAAHEIKTPQGRPPFAFKLHQFISGAGKVLTTLEAVGKRYITLDAQRFAPN
                     RQEEGVLLYTAHFCRECGQEYHPVWIDKTGFSPRDIAEISADDQDTNYGFLCPVNKQQ
                     LYGGIEDLPEEWLDFSKAEPKVKPAYKKSVPDHVWVNAQGQQGQGESYWLIQGKFRFC
                     LHCGQQYEAHGKDVNRLSSLSGEGRSSATTMLTLSALRLLFAETDIPEGVPDPRKLLG
                     FTDNRQDAALQAGHFNDFVFLLTLRSALIAALYNNNGVLIEGDLADAVFKALGFESTD
                     IATRAEYLRNPSLRGFALQDAQRTLRFILGYRLLRDLRKGWRFNNPNLDQLNLLEIDY
                     LQLDDFCADGGEFSSSLLQKIGAEGRTAFARILFDEMRRSLCLESSYLDAIHQDKTRT
                     SAFSQLNEHWSFMSDEKLDTARYLILGKRPESKGKPRTDLVTAGLRSKVLKQLKRAKF
                     WSENIVADQGSHVWKNDAEIVEAIEDFLTAALNYGYVKKQTVDSHVVGWRLNANLMAW
                     RLPMSEKANEDNSKLAKPNAFFRQLYLAIAELLRMPSHPLFNFEAHEHTAQVDSAKRQ
                     LLEMRFRYTDKDRQDWLKNAANDGALKRLPVLFCSPTMELGVDISALNTVYMRNVPPT
                     PANYAQRSGRAGRSGQQALVITYCTALSPHDQWFFHHATEMVHGVVRAPTLDLSNKDL
                     VDSHLHAVWLSCTEQALDNSIAPLLILGDSEKPEKPIIDSVWAKFNQPEILARAKHAS
                     AGVITQLKEELKYATWFTADYVGQTIDESPRQFNEALERWRKLYEATQKQMDLADKIV
                     NSPVVSANERESARRRYSDAARQFSVLLKTRNGQNTDFYTYRYLASQGFLPGYNFPRL
                     PLMAWIPASAKSNSSGKEDEGGMVSRPRFLALSEFGPRSLIYHEGRMYRVVRAKLNVG
                     ASEHISGSGQLGTLSAMVCSVCGYGHLGNVEEGQPLKNTCDNCQSPLTDHDWVHNLYR
                     IETVETVAVERISINDEERQRQGFELQTTYRFLPAKGADIQKTESEVWQNNEVVAELT
                     YSPTAQLWRINRGWRRRTDKKRLGFYINPITGLWSKQDTDDEDTSTDVESAQSKVANQ
                     RIVPFVEDHRNILILTPVQRLSIEAIATLQAALKRGIEQTFQI"
BASE COUNT         1435 a          832 c         1095 g         1361 t
ORIGIN      
        1 ttatcctgtt gtaaatacaa gtaataaatc atatagcagg ggcaacgaat ggcggaaatg
       61 attatcaaaa gtatcgcccg acatagttgc ttttgataaa aaagggctta tatgtataac
      121 gtatttgatt ttagaaataa cctaattaaa gactacagtt cattttctcg tagttttacg
      181 cgtattgcag cagttgatat tctaagtgaa gtcaatagcc agtatgataa tggcagatat
      241 tggcctgagc cgttaataca aattaatccg aattatcaac gcaaaggaac ggttcagcaa
      301 ttagcacaag agggtgtatt acacaaagag tgtgctgata tttttcaagt agaaaagctt
      361 tcgcatatcc ctcagccatt gcatctatat agtcatcaat tagaggctat tgctaaggca
      421 aaagcgaagc aaagttttat tgtgactaca ggtacaggtt cgggaaagtc gttatctttc
      481 tttattccca taatcgatca aattctaaaa gcaaaagatt tagataaaac acccagaact
      541 cgcgcaatta ttatttatcc catgaatgcc ttggcaaata gccaactaga agagctgaac
      601 aagtttcttt atggctatca acctgagcag cagcctttta ctgttgctcg ttatacagga
      661 caagaaaata atatagaaag agaaagtatt gctaaaaatc cacctgatat tttgttaaca
      721 aactttatga tgttggagct gattctaact cgttttgaag agaccgatcg gcatgttgta
      781 gctaactgtc agggtttgga ttttttggtg ctagatgagt tgcataccta tcgaggacgg
      841 caaggcgcag atgtagccat gttggttcgg cgtttaagag aacgcttaca agctaagaat
      901 ttaatttgta ttggaacatc ggcaacaatg tctagtacag gtacagtaga agaccaaaaa
      961 aacacggttg caggcgtcgc tagtaagctc tttgcaacga ccatcacggc aaacgatgtg
     1021 attggagaaa ccttagagcg agtcactgat actaatcgta atgttgacgc tatccgtttg
     1081 gagttggcgc attcagttaa tcgaaaagat ttttattggc caagttttac ggcgtttcag
     1141 cacgacccat tggctatttg ggtagagctt actttaggtg ttgaactacc taaagagggg
     1201 cgaccacgtc gagctaaacc taaaaccgtt tcagaagcgg caagtcaatt agctaaagat
     1261 gcagcttgta gcgaacaaca agccaaagaa gtattgcagt gtttcttggt ggcagcacat
     1321 gagatcaaaa cgccacaagg taggccgccg tttgcattca agttgcatca atttattagt
     1381 ggtgcaggta aggtgttaac taccttggaa gcagttggta aaagatatat tactcttgat
     1441 gcacaaaggt ttgcacccaa taggcaagaa gaaggcgtgt tgttatatac agcacatttt
     1501 tgtcgggagt gtgggcaaga atatcatcct gtttggatag ataagactgg tttttcgccg
     1561 cgtgatattg ctgaaataag tgcagatgat caagatacta attatggctt tttatgccca
     1621 gtgaataagc agcaacttta tggtggtata gaggatttgc ctgaagagtg gttggatttt
     1681 tcgaaagccg aacctaaagt taagcctgcc tacaaaaaaa gcgtacctga tcatgtttgg
     1741 gtaaatgcac aaggtcagca aggtcaaggt gaatcatatt ggctcattca aggtaagttc
     1801 agattttgtt tgcactgtgg gcagcaatat gaggcacatg gtaaagatgt taatcgctta
     1861 tccagcttat ctggtgaggg acgttcctca gcaacgacta tgctgacttt aagtgcttta
     1921 cgtttgttgt tcgcagaaac agatatacca gagggtgtgc cagaccctag aaagttatta
     1981 ggttttaccg ataatcgtca agatgcggct ttgcaagcag gccactttaa tgattttgtc
     2041 tttttattaa cgttacgcag tgcattaatt gcagccttat ataacaacaa tggtgtattg
     2101 attgaaggcg acttagctga tgctgtgttt aaggctttag gctttgaaag cactgatata
     2161 gctactcgtg ctgagtattt acgtaacccg tctttaaggg gttttgcttt gcaagatgcg
     2221 cagcgaaccc ttcgctttat tttaggctat cgcctattac gtgatttacg caaaggttgg
     2281 cgttttaata atcccaactt agaccaactc aatttactag aaattgatta tttgcaattg
     2341 gatgactttt gtgcagatgg tggtgaattt tcctcatcat tattgcaaaa gattggggct
     2401 gaaggacgaa ctgcttttgc tcgtattttg tttgatgaaa tgagacgttc attgtgttta
     2461 gaaagtagct atttagatgc aattcatcag gataaaacaa gaaccagtgc ctttagccaa
     2521 ttaaatgagc attggtcgtt tatgtcagat gaaaagctcg atactgcacg ttatctaatt
     2581 ttaggaaaga gacctgaatc taaaggcaaa cctcgcactg atttagtgac ggcagggctg
     2641 cgatccaaag tattaaagca gttaaagcgt gctaaatttt ggagtgaaaa tattgttgcc
     2701 gaccaaggta gtcatgtatg gaagaatgat gcagaaattg ttgaagcgat agaggatttt
     2761 ttaacggcag cgttaaatta tggctatgta aaaaagcaaa ctgtagatag tcatgtagtt
     2821 ggctggcgat taaatgccaa tttaatggcg tggcgattac ctatgtcaga gaaagctaat
     2881 gaagataaca gtaagttagc taagcccaat gcctttttta gacagctata tttagccatt
     2941 gcagagttat tgcgtatgcc ttcgcatccg ttatttaact ttgaggcgca tgagcatacg
     3001 gcgcaagtgg actcggctaa gcgacagtta ttagaaatgc gattccgtta caccgataaa
     3061 gacagacagg attggttaaa aaatgcggct aatgatggtg cattaaagcg gttgcctgtt
     3121 ttattttgct cgccaacaat ggagttgggt gtggatattt ctgcgctcaa tactgtgtat
     3181 atgcgtaatg tgccacccac tcctgctaat tatgcgcaac gtagtggccg tgctggacgt
     3241 tcagggcaac aagctttggt aattacttac tgcactgcac taagcccaca tgaccaatgg
     3301 ttttttcatc atgcgactga gatggtacac ggtgttgttc gtgcaccaac attagatttg
     3361 tctaataaag atttggttga tagtcattta catgccgtct ggttgtcttg tactgagcaa
     3421 gccttggata acagtattgc acctttgtta atactaggtg attcggaaaa accagaaaag
     3481 ccgattattg atagtgtttg ggcgaaattt aatcagccag aaattttagc acgcgcaaag
     3541 cacgcttcag caggtgtaat aacacaacta aaagaggaac ttaaatatgc cacatggttt
     3601 acagcggatt atgtcgggca gacaatagat gagtcaccgc gtcaattcaa tgaggcttta
     3661 gagcggtggc gcaagctcta tgaggcaact cagaaacaaa tggacttagc cgataaaatt
     3721 gttaatagcc ctgttgtgtc agcgaatgag cgagaaagtg cgcgtcgccg ttatagtgat
     3781 gcagcacgcc agttttcagt gttgctaaaa acaagaaatg gccaaaatac agatttttac
     3841 acctatcgtt atttagctag tcagggattt ttgcctggtt ataattttcc tcgtttgccg
     3901 ttaatggcat ggattcctgc aagtgctaaa tctaattcat caggtaaaga agatgaagga
     3961 ggtatggtca gtcggccacg atttttagca ttgtctgagt ttggcccacg aagtttaatt
     4021 taccacgaag gacgtatgta tcgtgttgtg cgagccaaat taaatgtagg agcaagcgag
     4081 catatttcgg gtagtggaca attaggtaca ttatcagcaa tggtgtgtag tgtgtgtggt
     4141 tatggtcatc taggtaatgt agaagaaggc caaccgttaa aaaatacctg tgataactgc
     4201 caatctccat taacagacca tgattgggta cataacttgt accgtataga aacagtagaa
     4261 acagtggctg tagagcgtat ttctattaat gatgaagaac gccaacgtca aggctttgaa
     4321 ctgcaaacga catatcgttt cttgccagct aaaggcgcag atattcaaaa aacggagtct
     4381 gaagtttggc aaaataacga agtggtagct gagttaacgt attctccaac agctcagctt
     4441 tggcgaatca atcgagggtg gcgcagacgt accgataaaa aaagattagg cttttatata
     4501 aatccaatta cgggattatg gagcaaacaa gatacagatg atgaggatac ttcgactgat
     4561 gtcgaatcgg cgcaaagtaa agtagccaat caacgtattg tgccattcgt ggaagatcat
     4621 cgaaatattt tgatattaac tccagttcag cgattgtcga ttgaggcgat agcaaccttg
     4681 caagcagcac tcaagcgagg cattgagcaa acctttcaaa tag
//
LOCUS       DAOQMB010000161         4719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8552_length_4719_cov_15.546527, whole genome shotgun sequence.
ACCESSION   DAOQMB010000161 DAOQMB010000000
VERSION     DAOQMB010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8552_length_4719_cov_15.546527"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /gene="ppk1"
                     /locus_tag="PLY05_06515"
     CDS             <1..2019
                     /gene="ppk1"
                     /locus_tag="PLY05_06515"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HNC02994.1"
                     /translation="LSILDFHLRVLEQAVDTRHPLLERLNFLLIFSRNLDEFFEIRVA
                     GLLQQLSFDAASVSPDGLLVEDVLKRIAETSHKAVEKQYRILNDDLFPALAAENIRFL
                     KRDELNETQAHWVKHYFREQVLPVLTPISLDPAHPFPRLVNKSLNFIVSLEGKDAFGR
                     QISMAVVPAPRSLPRVVRLPDELTNGGDQHVLLSAIIHEHVSELFPGMVATGCYQFRV
                     TRNADLTVDEDADDLALALKGGLYSRRFGKAVRLEVADNCPKTISDYLLRKFGLKDQS
                     LYRVNGPVNLARLLSNFNRPRLRYTPFTPSIPSIVQKSESVFEALKKQDVLLHHPFES
                     FTPVINLLREAARDPQVLAIKQTLYRTGADSEIVNILAEAARHGKEVTAVIELRARFD
                     EESNIEVANKLQEAGAIVVYGIVGYKTHAKMILVVRREDNKLHRYVHLGTGNYHAVTA
                     RIYTDYGLMTANPELCEDVHKIFQELTGMGKTAKLKKLLHAPFTLHAQLIAYIEQEIR
                     HAQAGKAAHIIIKVNALTEKQLIQALYRASMAGVKVDLIIRSICCLRPQLAGVSENIR
                     VRSIVGRFLEHTRVFYFANDGEAKVYCASADWMDRNLFSRVEACFPIDDPTLKKRIIK
                     DGLLNYLTDNQLAWELQADGSWQRVQSAEGELPHQAQALLLEKLAKLA"
     gene            2099..3391
                     /locus_tag="PLY05_06520"
     CDS             2099..3391
                     /locus_tag="PLY05_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005089704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNC02995.1"
                     /translation="MDFVNNLFVIIALIASSAFFSCAEIALASSRKIRLQQLVEAGNV
                     NAAKVLALQEEPGNFFTVIQVALNAIAIMGGILGEQAFSPYIATLLENVFGKTAWLEQ
                     VSFLCSFTIVTSLFIIFADLMPKRLAIIYPEQFAVAIVKPILFFLGVLKPIVRVYDGI
                     ANLLFKLLNIKTDVEDAITFDDVSAMVDAGTASGALHKQEQHLIENVFELDTRTVTFA
                     MTSREHVIFLRLQDDEQMLTAKISDYPHSRFLVCNDHIDNVLGYVESKDILKRTLKQQ
                     AISLNNELQLKNLLAIPDTLTLSELLERFKVTNQDLAVILNEYALVVGIVTLNDVMKT
                     LMGDLVSPFIEEQIVKRDDNSWLIDGVTPIEDVRRALSIDSFPNEENYQTIAGLMMYL
                     LKKIPKRTDFIEYAGYKFEVVDIDNYRIDQLLVTKILS"
     gene            3394..3861
                     /gene="nrdR"
                     /locus_tag="PLY05_06525"
     CDS             3394..3861
                     /gene="nrdR"
                     /locus_tag="PLY05_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HNC02996.1"
                     /translation="MHCPFCRAPDTKVIDSRLVGEGEQVRRRRECVICGERFTTFEGA
                     ELVTPRVIKSDGSRVPFDDNKLRKSMMHALQKRPVSIEKIDSAIQFILHKLRASGERE
                     VKSIVIGELVMAELAKLDKVAYVRFASVYRDFQDVEAFNTEIEKLKQHESDHE"
     gene            3842..>4719
                     /gene="ribD"
                     /locus_tag="PLY05_06530"
     CDS             3842..>4719
                     /gene="ribD"
                     /locus_tag="PLY05_06530"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNC02997.1"
                     /translation="MSPTMNDQLYMARAIQLAQQGLYTTHPNPRVGCVIVKDGQIVGE
                     GFHARAGQAHAEVFALRQAGELAQGATAYVTLEPCAHFGRTPPCANALVEAKVARVVM
                     ASLDSNPLVAGKGQAILENAGIQTRVGVLEDQAKALNKGFFRRMQGGLPYVRLKTAAS
                     LDGRTAMQSGESKWITGTDARKDVQKLRAQSGAVITGIGTVLADNPSLTVRPDDWLDW
                     HYGDVVQPLRVVFDSQLATPIDAQLLSTQGVIIVTQQSLEHPKARALQTKGVEIWSFA
                     DREGVLQQVLTKLAQRG"
BASE COUNT         1323 a          845 c         1077 g         1474 t
ORIGIN      
        1 ctcagtattt tagattttca cttgcgtgtc ttagagcaag cggttgatac gcgtcatcct
       61 ttattagaac gattaaactt tttattgatt tttagtcgta acttagatga gttttttgaa
      121 attcgagttg cagggttatt gcaacaatta tcatttgatg cagccagtgt ttcacctgat
      181 ggtttgttag tcgaagatgt tttaaaacgt attgctgaaa cctcacataa agcggttgaa
      241 aaacaatatc gtattcttaa tgatgattta tttcctgcct tagccgctga aaatattcgc
      301 tttttaaagc gtgacgaact aaatgaaact caagcgcatt gggttaaaca ttattttcgt
      361 gagcaagtgt tacctgtgtt aacaccgatt agcttagatc ctgctcatcc atttccgcgt
      421 ttagtcaata aaagtcttaa ctttattgtc agtttagaag gaaaggatgc ttttggccga
      481 caaatttcaa tggcggttgt gcctgcacca cgttctttgc cgcgtgtggt gagattgcct
      541 gatgaattaa ccaatggtgg cgatcaacac gttttgttat cggcgattat tcatgaacat
      601 gtcagtgagt tatttccggg tatggtggcg actggatgtt atcaattccg tgttacccgt
      661 aacgccgact taacggtgga tgaagatgcc gatgacttag ccttagcgtt gaaaggtggc
      721 ttatattcgc gtcgttttgg taaagcggtg cgtttggaag ttgccgataa ttgcccaaaa
      781 acaattagcg attatttgtt gcgtaagttt ggtttaaaag atcagtcgtt gtatcgtgtc
      841 aatgggcctg tcaatttggc gcgtttatta tccaatttca atcgaccacg tttgcgttat
      901 acgcccttta cacccagtat tccgagtatt gtacaaaaat ctgaaagtgt gtttgaagcc
      961 ttaaaaaaac aggatgtgtt attgcatcat ccttttgaat catttacacc agtgattaat
     1021 ttgttgcgtg aagcggcacg agatccgcaa gtattagcga ttaaacaaac tttgtatcgt
     1081 acaggtgcag actcagaaat tgtcaatatt ttggccgaag ccgcacgtca tggtaaagag
     1141 gtcactgcgg tgattgagct acgcgctcgt tttgatgaag agtccaacat cgaagtagcc
     1201 aataaattac aagaagcagg ggcaattgtc gtttatggca tagttggtta taaaactcat
     1261 gccaaaatga ttttggtggt gcgtcgagaa gataacaaac ttcatcgtta tgtacattta
     1321 ggcacaggca attatcatgc agtcaccgca cgcatttata ccgattatgg tttaatgacg
     1381 gcgaatcctg aactctgtga agatgtgcat aaaattttcc aagaattaac aggcatgggt
     1441 aaaactgcaa aactcaaaaa attgcttcat gcgccattta ccttacacgc acaattaatt
     1501 gcctatattg agcaagaaat tcgtcatgcc caagcaggaa aagcagctca tattattatt
     1561 aaagtcaatg ccttaactga aaaacaatta attcaggctt tatatcgcgc ttcaatggca
     1621 ggggtgaaag ttgatttaat tattcgctcg atttgttgtt tacgtccgca attggcaggt
     1681 gtgtcagaaa atattcgtgt gcgttcaatc gttggacgct ttttagaaca tacacgcgtg
     1741 ttttattttg ctaatgatgg cgaggcaaaa gtgtattgcg ccagtgccga ctggatggat
     1801 agaaacttgt tttctcgagt tgaagcctgt ttcccaatcg atgatccgac cctgaaaaaa
     1861 cggattatta aagatggttt gttaaattat ttgaccgata atcaattggc atgggaacta
     1921 caagcggatg gtagttggca acgtgtgcaa tctgccgaag gggagttgcc acatcaagcg
     1981 caagcattat tgctagaaaa attagctaag ctggcttagg gtatctcaaa aggcgcaagg
     2041 ttgcgccttt cttatgtttg tctatattat gagggcatat tcatattttt gagccatcat
     2101 ggattttgtc aataatttat tcgtcatcat tgctttaatt gccagtagtg cgtttttttc
     2161 gtgtgccgag attgccttgg cttcatcgcg taaaattcgt ttacagcaat tagttgaagc
     2221 aggcaatgtt aacgccgcca aagtgttagc tttgcaagaa gaaccgggta atttttttac
     2281 cgtgattcaa gtcgcactta atgccattgc gattatgggc ggtattttag gcgaacaagc
     2341 tttttcgcct tatattgcca cgttgttaga aaatgtcttt ggtaaaactg cttggttaga
     2401 gcaagtcagc tttttatgtt cattcaccat tgttacctca ttatttatta tttttgccga
     2461 cttgatgccc aagcgtttag ccattattta tcccgaacaa tttgcggtgg cgattgttaa
     2521 accaattctg ttttttttgg gcgttttaaa gcctattgtt cgtgtttatg atggtattgc
     2581 aaatttattg tttaaattat tgaacattaa aaccgatgtt gaagatgcta ttacctttga
     2641 tgatgtgtcc gcgatggttg atgcaggcac agcatcaggt gcattgcata aacaagagca
     2701 gcatcttatt gaaaatgtct ttgagttaga tacccgcacc gtaacctttg ccatgacttc
     2761 gcgtgaacat gtgatttttt tacgcttaca agatgatgag caaatgttaa cggctaaaat
     2821 cagtgattat ccgcatagtc gatttttggt gtgtaacgat catattgata atgttttggg
     2881 ctatgttgaa tcgaaagaca ttttaaaacg aaccttaaaa caacaagcga tttcattaaa
     2941 taacgagtta cagcttaaaa atttattggc aattcctgat accttaacct tgtcggagtt
     3001 attagagcga tttaaagtca ctaaccaaga tttagcagtg attttaaatg aatacgcctt
     3061 agtggtgggg atagtcaccc tgaatgatgt gatgaaaact ttaatgggcg acttggtgag
     3121 tccttttatt gaagaacaaa ttgttaaacg tgatgataat tcgtggttaa ttgatggtgt
     3181 cactcccata gaagatgtac gccgcgcctt gtcaattgac agttttccta atgaagaaaa
     3241 ttatcaaacc attgctggtt taatgatgta tttattaaaa aagattccca agcgtaccga
     3301 ttttattgag tatgcaggtt ataaatttga agtggttgat attgataatt atcgcatcga
     3361 ccaattatta gtgaccaaaa tattgagtta attatgcatt gtccgttttg tcgagcacct
     3421 gatactaagg tgattgactc tcgcttagtg ggcgagggtg aacaagttcg tcgtcgccgt
     3481 gagtgtgtga tttgtggtga acgctttacc acgtttgaag gggcagaatt agtcacgcca
     3541 cgggtgatta agtccgatgg tagtcgtgtg ccatttgatg ataataaatt gcgtaaaagc
     3601 atgatgcacg ccctacaaaa acgccctgtt agcattgaaa aaattgatag tgcgattcaa
     3661 ttcattttgc ataaattacg agcatcgggt gagcgtgaag taaaatctat tgtcattggt
     3721 gagttggtca tggcggaatt ggcaaagctt gataaagtgg cttatgtccg ttttgcctcg
     3781 gtctatcgtg actttcaaga tgttgaagcc tttaacaccg aaattgaaaa gctcaaacag
     3841 catgagtccg accatgaatg atcagcttta tatggcacgt gccattcaat tggcacaaca
     3901 aggtttgtat accactcatc ctaatccgcg tgtcggttgt gtgattgtta aggatggcca
     3961 aattgttggc gaaggctttc atgcgcgtgc tggtcaagct catgccgaag tgtttgcttt
     4021 acgtcaggct ggtgaattag cacaaggtgc aacagcgtat gtgaccttag agccttgtgc
     4081 tcattttggc cgaacaccac cgtgtgccaa tgccttagtc gaggcaaaag tagcacgagt
     4141 ggtgatggcg agtttagata gcaatccctt ggttgcaggt aaaggacaag caattttaga
     4201 aaatgccggc attcaaacaa gggttggtgt attagaagac caagccaaag ccttaaataa
     4261 aggctttttt agacgaatgc aaggcggctt gccttatgtg cgcttaaaaa ccgccgccag
     4321 cttagatggt cgaactgcga tgcagtcagg tgaatcgaaa tggattactg gtacagatgc
     4381 acgcaaagac gtacaaaaac tacgcgcaca aagtggggca gtgattacag gaattggcac
     4441 tgtattagcc gataatccaa gtttaacggt tcgccctgac gattggttag attggcatta
     4501 tggcgatgtg gtgcagccat taagagtggt gtttgattcg caattagcga ctcctattga
     4561 tgcccaacta ttgtccacac aaggcgtgat tattgtcacg cagcaaagtt tagaacatcc
     4621 caaagcaaga gctttgcaaa ctaaaggcgt ggaaatatgg tcttttgcgg atagggaggg
     4681 ggttttacag caagtattaa ccaaacttgc tcaacgtgg
//
LOCUS       DAOQMB010000162         4674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8679_length_4674_cov_14.940030, whole genome shotgun sequence.
ACCESSION   DAOQMB010000162 DAOQMB010000000
VERSION     DAOQMB010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4674
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8679_length_4674_cov_14.940030"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1284)
                     /locus_tag="PLY05_06535"
     CDS             complement(256..1284)
                     /locus_tag="PLY05_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HNC02998.1"
                     /translation="MSVLPSVSQFPELHQLHQLMNQYPNQAKYHLLTHINVKQFKLPI
                     WSISLGSEDPTHPIIGFFGGVHGMERIGSQVLLAWLQNLLSRTTWDISLQHLLTQVKI
                     VFIPLLNVGGLLNNTRSNPNGVDLMRNAPIDAQDKTLWPLAGQRLSKRLPWYRGKQQQ
                     MELESQTLVNFVKQELLNRPFALAIDCHSGFGLRDRIWFPYAAHKQPPYHLAEAVALR
                     EIFNQSYPHHDFYIMEPQSLNYTTHGDLWDYLYDYQLNNQNNHLFMPFTLEMGSWLWV
                     KKNPQQLFNKTGLFNPILPHRLTRVLRRHLSLFDFLLKATASYQQWIPHNHHRLHLTN
                     IGIERWYT"
     gene            complement(1334..1855)
                     /locus_tag="PLY05_06540"
     CDS             complement(1334..1855)
                     /locus_tag="PLY05_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC02999.1"
                     /translation="MLSLLKKLAFISLAGLALPALAEQRPIQFPEYNSALTISGYTAQ
                     SLHSTHTPGWGIGGETTYSRDHFNQSGGFVYFPEGTDAPVFNLFIGTGYKRHAQFQVG
                     YGTENAVYRFRSENDLAYLWVLSRNLMNLNLTLPTRYDHYPNNRWVAIFSIEKYDDHT
                     YLDNISLGLGLRY"
     gene            complement(1977..2927)
                     /gene="corA"
                     /locus_tag="PLY05_06545"
     CDS             complement(1977..2927)
                     /gene="corA"
                     /locus_tag="PLY05_06545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HNC03000.1"
                     /translation="MLMAFSLNKGAIEQIAINAAADLGEHVIWVDLINPTEEERDWLR
                     VAYAQELPTIDDLYEIEASSRFYENEFGLHISSYFLNYADKNPENISAAFIFNGDRLF
                     TLREEELAAFRLFRMRGRKGLVNINNSKSILLGLFETKVENLADILERVHADLEETSQ
                     QVLGNAENDDMETLLTNIATQEDINGKVRISMMDLQRMLYLLLRAGMLNAEQSERLRD
                     IIRDSESLIAHSSFLFDKIKFLLDTTLGFINVNQNKIIKMFSVASVMFLPPTMVASVY
                     GMNFEFMPELKWQHGYLFGLFLMFLSAVVPFLYFKRRRWL"
     gene            complement(2995..3687)
                     /locus_tag="PLY05_06550"
     CDS             complement(2995..3687)
                     /locus_tag="PLY05_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNC03001.1"
                     /translation="MRLFFNQILASQALCLCCQYQKQAKFGFCLACYQDLPHLPRPCL
                     YCGLPLNEDNLCTCHPEDWPFTACLSAFEYQFPINKLIGQYKNQARLGLCQSFAQSLA
                     AQIKKQKQPLPQLLIPVPLSPQKLAKRGFNQSLELAKDLGKILSIPVDYQLLQTVGQA
                     KSQKELSKKQRQNLNAEQFYLSNKIQQTHIAIIDDVITTGATVKTIAQILQKQGVTHI
                     QAWSIARVMTKQ"
     gene            3777..>4674
                     /gene="bioB"
                     /locus_tag="PLY05_06555"
     CDS             3777..>4674
                     /gene="bioB"
                     /locus_tag="PLY05_06555"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_249191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HNC03002.1"
                     /translation="MTTLRHDWNRAELRALFDLPFNDLLFQAQTIHRQHFDANAVQVS
                     TLLSIKTGACPEDCKYCSQSGHYDTGLDKEKLLEIEKVVQEAQAAKDKGASRFCMGAA
                     WRSPRDKDMPYVLEMVKRVKGLGLETCMTLGMLAPEQAQALGEAGLDYYNHNLDTSPE
                     YYGKIITTRSYNDRLNTLANVRDAGIKVCSGGIIGMGEKREDRIGLLMELANLPAHPD
                     SVPINMLVKIAGTPLGDVEDLEPFEFVRTIAVARILMPKSFVRLSAGREKMNEQLQAL
                     CFFAGANSIFYGEKLLTTSNPET"
BASE COUNT         1460 a          815 c          973 g         1426 t
ORIGIN      
        1 gctaccaaaa ctaatcctaa taatgactta cgcataggat aaactccttt gtatggataa
       61 aattagccta atggctttaa tagctcaaat cgtagtctaa tcgctagcga ttggcaagca
      121 aaaagatgtt attgattata tcaaaatgat gatcggttca tttttgccgt tgtatggcaa
      181 tcaatgaaat cttgtattga taatattata taccagctca atttaatcgt gactttgtta
      241 aacaatttgt cacacttatg tataccaccg ttcaatgcct atattagtca aatgtaggcg
      301 atgatgatta tggggtatcc attgctgata agaagccgtt gcttttaata aaaaatcaaa
      361 taagctgaga tgacgacgta atacccgtgt taaacggtgt ggcaagatgg gattaaataa
      421 acctgttttg ttaaaaagtt gctgaggatt ttttttgacc cataaccaag accccatttc
      481 aagcgtaaat ggcataaata aatggttgtt ttgattgttt agttggtagt catacaaata
      541 atcccataag tcgccatgtg tggtgtagtt tagcgattgt ggttccatta tgtaaaaatc
      601 atgatgtgga tagctttgat taaaaatctc acgcaaggca acggcttctg ctaagtgata
      661 aggtggttgt ttgtgtgccg cataaggaaa ccaaattctg tcgcgtaagc caaaacctga
      721 atggcaatca atagccaagg caaatggtcg attaagtaat tcttgtttga caaaattgac
      781 taaagtttgt gactccagct ccatctgttg ttgttttccc cgataccaag gcagcctttt
      841 tgataatcgt tgtcctgcta atggccataa ggttttatct tgagcgtcaa ttggtgcatt
      901 acgcattaaa tccacgccat taggattgct acgtgtattg ttgagtaagc cacccacatt
      961 taataaggga ataaagacaa tttttacttg tgttaataaa tgctgtaaag aaatatccca
     1021 tgttgttcga ctgagtaagt tttgtaacca cgcaagtaat acctgacttc caatacgctc
     1081 catgccatgt acgccaccaa aaaaaccaat aatgggatga gtagggtctt cactacctaa
     1141 actgatagac caaatgggca atttaaattg tttgacatta atatgggtga gaaggtggta
     1201 cttcgcttga ttaggatatt gattcatcag ttgatgaagt tggtgtaact caggaaattg
     1261 gctgacagag gggagaacag acataatcaa catccagact tagagtggtg aactctaagt
     1321 ctgttagtaa tgtttaataa cgcaaaccta aacctaaaga aatattatct aaataggtgt
     1381 ggtcatcata tttttcgatg gaaaaaatgg caacccaacg attattaggg taatgatcgt
     1441 aacgggttgg taaagtgaga ttcaaattca ttaaattacg acttaaaacc cataaataag
     1501 ctaagtcatt ttcacttcta aagcgataaa ccgcattttc tgtgccataa cctacttgaa
     1561 attgagcatg acgtttgtag cctgtcccaa taaataaatt aaatactggc gcatctgtgc
     1621 cttctggaaa ataaacaaaa ccaccacttt gattgaaatg atcacgggag taggtggttt
     1681 ctccaccaat accccaacct ggtgtgtgag tgctgtgtaa actttgggca gtataaccac
     1741 tgatggttaa agcagaattg tattcaggaa attgaatcgg gcgttgttcg gccaatgcag
     1801 gcaaagcaag gcctgctaaa gaaataaagg ctagtttttt taagagtgat agcatggcat
     1861 acattccgtg cgaagcaaaa atcaggcgca ttatgccgtt ttttatgctt ttgggctatg
     1921 gttagataca ctttgcgtga tgatagatgt tgttttgtta tggttttaac aaaaaatcac
     1981 aaccaacggc gacgtttaaa atataaaaag gggactactg ctgataaaaa catcaagaat
     2041 aaaccaaaca aatagccatg ttgccatttg agttcgggca taaactcaaa gttcatgcca
     2101 tagacactag caaccatcgt tggtggcaaa aacatcaccg aggcaaccga gaacatttta
     2161 ataattttgt tttggttgac gttaataaaa ccaagcgtgg tatcgagtaa gaatttaatt
     2221 ttatcaaata aaaatgagct atgagcaatc aaggactcag agtcgcggat aatatcgcgt
     2281 aaacgttcac tttgctcagc attaagcata cctgcacgta acaatagata aagcatacgt
     2341 tgtaaatcca tcatcgagat acgtacttta ccattaatat cttcttgggt tgcaatattg
     2401 gtcaacaagg tttccatgtc gtcgttttcg gcattgccta gaacttgttg actggtttct
     2461 tctaaatcgg catgaacacg ttcgagaata tccgctaaat tttcaacttt ggtttcaaac
     2521 aagcccaata aaatggattt ggaattatta atattgacca aacctttgcg gccacgcatc
     2581 cgaaacaaac gaaatgcggc tagttcttct tcgcgcaggg taaataaacg atcgccatta
     2641 aaaataaatg cggcagaaat attttcggga tttttatcgg cataatttaa aaagtatgag
     2701 ctaatgtgta agccaaattc gttttcataa aaacgagagc tagcctcaat ttcatataaa
     2761 tcatcaatgg ttggtagttc ttgagcataa gcaactctta accagtcacg ctcttcttcg
     2821 gtgggattaa ttaaatcaac ccaaatgaca tgttctccca aatcagcggc agcattaatg
     2881 gctatttgct ctattgcgcc tttgttgagg gaaaaagcca ttaacataaa tatgctcacc
     2941 ttgatgagtt gtgggtgtaa ttggtcattt tagcaaaaaa gttttacaaa ttgtttactg
     3001 ttttgtcatc actcgtgcaa ttgaccacgc ttgtatatga gtgactcctt gtttttgcaa
     3061 tatttgtgca atcgttttaa cggttgcgcc tgtcgtaatc acgtcatcaa taattgcaat
     3121 atgcgtttgt tgaatcttgt tgctgagata aaattgttcg gcattaaggt tttgtcgttg
     3181 ttttttagat agctcttttt ggcttttagc ttggcctacg gtttgtaaga gctggtaatc
     3241 tacaggaata gacaaaattt tgcccaaatc ttttgctaat tcaagacttt gattaaaacc
     3301 acgtttagcg agtttttgcg gacttaaagg gactggaatt aataattgag gtaaaggttg
     3361 tttttgtttt ttaatttgag cggccaacga ttgagcgaaa ctttggcaca aacccaaacg
     3421 cgcttgattt ttatattggc cgatgagttt attaatcgga aattgatact caaaagcact
     3481 taaacaggca gtaaaaggcc aatcttcggg gtggcaagta cacaaattat cttcgtttag
     3541 gggtaaacca caatataaac aaggacgagg gaggtgaggt aaatcttgat aacaagctaa
     3601 acaaaaacca aattttgctt gtttttgata ttggcagcac aaacacaagg cttgagaagc
     3661 taatatttga ttaaaaaata atctcatgta aactttttaa tcctttaaaa gttgacggtg
     3721 ggtgatttta tcggcagtat caggcgtata aagctaattt taggatcata tacaaaatga
     3781 cgactttacg ccacgattgg aatcgcgctg agttacgcgc tttgtttgat ttaccgttta
     3841 atgatttgtt atttcaagcg caaactatcc atcgtcagca ttttgatgcc aatgcggtac
     3901 aagttagcac gttgttgtcg attaaaacag gcgcgtgtcc cgaagactgt aaatactgtt
     3961 cgcagtcagg tcattacgat acaggtttag ataaagaaaa gttattagaa atcgaaaaag
     4021 tggtgcaaga agcacaagca gccaaagaca aaggtgcaag ccgtttttgt atgggcgcag
     4081 catggcgtag tccacgcgat aaagatatgc cttatgtgtt ggaaatggtg aagcgcgtta
     4141 aaggtttggg tttagaaacc tgtatgacct tgggtatgtt agcgcctgaa caagcgcaag
     4201 ccttgggtga agcaggcttg gattattaca accacaattt agacacatca cctgaatatt
     4261 acggaaaaat tattactaca cgtagctata acgaccgttt aaatactttg gccaatgtcc
     4321 gtgacgcagg cattaaagtc tgttcgggtg gcattattgg catgggcgaa aaacgtgaag
     4381 accgtattgg cttgttaatg gaattggcga atttacctgc tcatcctgat tctgtgccaa
     4441 ttaatatgtt ggtcaaaatt gcaggcacac cattaggtga tgtagaagat ttagagccgt
     4501 ttgagtttgt gcgtacgatt gcggtggcac gaattttaat gcctaagtcc tttgtgcgtt
     4561 tgtcggcagg ccgtgaaaaa atgaatgagc aattacaggc tttatgtttt tttgctggcg
     4621 caaattcgat tttttatggt gaaaagctgt taaccaccag taaccccgaa accc
//
LOCUS       DAOQMB010000163         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8713_length_4662_cov_17.056870, whole genome shotgun sequence.
ACCESSION   DAOQMB010000163 DAOQMB010000000
VERSION     DAOQMB010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8713_length_4662_cov_17.056870"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLY05_06560"
     CDS             <1..720
                     /locus_tag="PLY05_06560"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HNC03003.1"
                     /translation="LSACDKPQAPKPVEKPTPVATTPSYAPLTIKNETWKKNKPPCKG
                     ENCPYIEVELQRVEDAALNTLIEKQLVNLVYIEDKQPTFADMSQLSQYFWPKTEGQWN
                     IYLQSKILRQKQHLLVVELNSDTYLGGAHGSPVTKYLNINRQNQQLLTLKDVLVTGQE
                     QAFWALVQKLHQQWLQENELTDPDLMTAWPFVTTDNIALGEKGIIVKYQAYAIAAYAF
                     GQPEFVVPYEQLKGIVKPEFL"
     gene            720..1538
                     /locus_tag="PLY05_06565"
     CDS             720..1538
                     /locus_tag="PLY05_06565"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005255151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNC03004.1"
                     /translation="MWIDTHCHFDIEEFAHDRAQVAADAYAQGVEAIAVIAYLAKYWP
                     QLLSVCQQFKQPQLLPVLGLHPCYISEHQNSDLGQLEQHLQNNDCIAIGEIGLDTYVA
                     EIKQPEFYQKQQQIFAEQLALAKQYQKSVVLHVRKAHADVFKIIKQQKFSEGGIVHAF
                     SGGVEEAKTYAKLGFKLGIGGSVTYPQAKRLHSVVNAMPIESLVIETDAPDMIPMPHR
                     QENAKYTRNSPAYLPSVAHALAQAKQLSSEQLAQILWKNSCQALHLSLKSSGFH"
     gene            1600..2376
                     /locus_tag="PLY05_06570"
     CDS             1600..2376
                     /locus_tag="PLY05_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HNC03005.1"
                     /translation="MQGLFERTHILLGDEKIERLKNARVFIAGLGGVGSFAAEALARM
                     GVGHLTLVDCDVVAASNVNRQLVALNSTVGQNKADIMAARVRDINPDVQLMVMTDFMN
                     RELFEAHFAQNQYDYVLDCIDSLNCKVALIEVAYKLGLNVASSMGAGGKTDPSQIKVS
                     DLYDTNVCHLASKMRQRLKRLNVGTGIRTVYSTEPPLPPLPPAPTSAGRPRAVNGTVS
                     YLPPLFGFTLAGLVVRDLTGISEPLKRKNVRKGYKPKGMT"
     gene            2531..3139
                     /locus_tag="PLY05_06575"
     CDS             2531..3139
                     /locus_tag="PLY05_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC03006.1"
                     /translation="MNWQSLWQSLNLVPPTNLLAELQTAYAQAQRFYHSQQHLQECLS
                     LLNEVEHLAKQPAELALALYFHDAVYNPQANDNEAQSAAWAVQVLTQCHAPQTLIQRI
                     EQLIIATQHHTIAKDDFDGQLLLDIDLAILGQESTRFNEYEQQIRQEYAWVSEQDYRI
                     GRSKVLQSFLQRKVIYQTDYFRQRFEQQARMNIQTALEGLAN"
     gene            complement(3141..4322)
                     /locus_tag="PLY05_06580"
     CDS             complement(3141..4322)
                     /locus_tag="PLY05_06580"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HNC03007.1"
                     /translation="MSHDPIVIVNGARTPMGGFQGSLSSVTAPELGAVAIKEAIARAG
                     IQPEDVQEVIMGCVLPAGLKQGPARQAMLKAGLPAATGATTINKLCGSGMKATMFAHD
                     LLVAGSVDIAVAGGMESMTNAPYVLTKARGGYRMGHGDIKDHMFLDGLEDAETGRLMG
                     SFAQEMATKLELTREQMDDFAIASLKRAQKAIAEGRFEAEIVGVPVKGRNGDTLVAID
                     EQPGNAKIEKIPSLKPAFAKDGTITAANASSISDGASALVLMRESTAKARGLQPLARI
                     VAHSTQSQHPSEFTIAPIGAMEKLFAKTGWNVADVDLFEINEAFAMVTMAAMIKHNIP
                     HDKVNVHGGACALGHPVGSTGSRIILSLIHALKQYGGKRGVASLCIGGGEATAMAIEL
                     V"
BASE COUNT         1471 a          934 c          971 g         1286 t
ORIGIN      
        1 ttatcggctt gtgataagcc ccaagccccc aaacctgtag aaaaacctac acctgtggct
       61 acaaccccca gttatgcacc gttaacgatt aaaaacgaaa catggaaaaa aaataaacca
      121 ccatgtaaag gcgagaattg tccgtatatc gaagtggaat tacaacgagt ggaggatgct
      181 gcacttaaca ctttgataga aaaacaatta gtcaatttgg tgtacataga agataagcag
      241 ccaacatttg ccgatatgag tcagctaagc cagtattttt ggccaaaaac cgaaggccaa
      301 tggaatatct atttacaatc aaaaatctta cgtcaaaaac agcatttatt agtagttgaa
      361 ctgaatagcg atacctattt aggtggcgca catggctcgc cagtgacgaa gtatttaaat
      421 attaatcgtc aaaatcaaca attattaacg ttaaaagatg tgttagtcac aggccaagaa
      481 caagcatttt gggcattagt acaaaaacta catcagcaat ggttgcagga aaatgagtta
      541 acggaccctg atttaatgac agcatggcca tttgtgacta ctgataatat tgctcttggc
      601 gaaaaaggca tcattgttaa atatcaagcc tatgccatcg cggcttatgc ttttggtcag
      661 cctgaatttg ttgtgcctta tgagcaatta aaagggattg ttaaaccaga gtttttgtaa
      721 tgtggataga tactcattgc cattttgata ttgaagaatt tgcccatgac cgcgcacaag
      781 tcgcggctga tgcctatgct caaggggtag aagcgattgc ggtcattgcc tatttagcca
      841 agtattggcc acaactacta tcggtttgtc agcaatttaa gcaaccacaa ttattaccag
      901 tgttgggttt gcatccttgt tatatcagcg aacatcaaaa cagcgattta ggacaactag
      961 aacaacattt gcaaaacaat gactgtattg cgattggtga aataggttta gacacttatg
     1021 ttgccgaaat caaacaacct gaattttatc aaaagcaaca acaaattttt gctgaacaat
     1081 tagccttagc caaacaatat caaaagtctg tggtgttaca tgtacgcaaa gcacatgcag
     1141 acgtctttaa aattattaaa caacaaaaat ttagcgaagg tggcatcgtt catgcttttt
     1201 cgggtggggt agaagaagca aaaacctatg cgaaattagg ctttaagttg gggattggtg
     1261 gcagcgtaac ctatccacaa gccaagcgtt tacatagcgt ggtgaatgcc atgccaatag
     1321 aaagtttggt gattgaaacg gatgcacccg atatgattcc catgcctcat cgtcaagaga
     1381 atgccaaata tacacgtaac tctcccgcct atttacccag tgttgcccat gctttagctc
     1441 aagccaaaca gctttcctca gaacagcttg cacaaatttt atggaaaaat agttgccaag
     1501 ccttgcattt atcgttaaaa agcagcggct ttcattaagc gtatttttta atatctatcc
     1561 tcgctacagg aggatgagat ttttatttag ggtgatttta tgcaaggtct gtttgaacgt
     1621 acccacatcc tattagggga tgagaaaatt gagcgtttaa aaaacgcgcg tgtttttatt
     1681 gcgggtttag ggggagttgg ctcgtttgct gccgaagcct tggcgcgtat gggcgttggc
     1741 catttaactt tagttgattg tgacgtggtt gctgctagta atgttaatcg tcaattagtg
     1801 gctttaaact ccaccgttgg ccaaaacaaa gccgatatta tggctgcacg tgtgcgtgat
     1861 attaatcctg atgtgcagtt gatggtgatg actgatttta tgaatcggga gttgtttgaa
     1921 gctcattttg ctcaaaatca atatgactat gtgttagatt gtattgattc tctcaactgt
     1981 aaagtggcgt tgattgaagt cgcttataag ttgggcttaa atgtggcttc tagtatgggg
     2041 gcaggtggca aaactgaccc ctcacaaatt aaagtcagcg atttatatga cactaatgtt
     2101 tgtcatttag ccagcaaaat gcgtcaacgt ttgaagcgtt taaatgtggg tacaggcatt
     2161 cgtacggtgt attctacaga gccaccctta ccgccgttgc cgcctgcacc cacatccgca
     2221 ggacgaccaa gagctgttaa tggcacagtc agttatttgc caccgttatt tggttttact
     2281 ttggcaggtt tagtggtacg cgatttaact ggtattagtg aaccattaaa acgcaaaaat
     2341 gtgcgtaaag ggtataagcc taaaggcatg acttaagaaa aataggagta ggcgtaggat
     2401 gggttgaggc acgaaaccca tcacattaat tagataaatc gcaggtttcc ttcgtcaacc
     2461 acacgctacc aaatgatgaa aacacattaa actaacgagt gcatcacttt aaatctaggg
     2521 aggaaattaa atgaactggc aatctctgtg gcaaagccta aatttagtgc ctcccactaa
     2581 tttattagca gaattacaaa cagcttatgc acaagcacag cgtttttatc acagccaaca
     2641 acatctacaa gaatgtttaa gcctattaaa tgaggtagaa catttagcca aacaacctgc
     2701 cgaattagcc cttgcgttat attttcatga tgcagtttat aacccacaag ccaacgacaa
     2761 cgaagcacaa agtgccgcat gggctgtgca agtattaact caatgccatg caccacaaac
     2821 attaatacag agaattgaac agttaattat agccactcaa catcacacta ttgccaaaga
     2881 tgattttgat ggccaattat tattggatat agatttagcg attttagggc aagaatcgac
     2941 tcgctttaat gaatatgaac aacaaatccg ccaagaatat gcgtgggtca gcgaacaaga
     3001 ttatcgtata ggtaggtcaa aggtattaca gagtttttta caacgaaagg ttatttatca
     3061 gactgattat tttagacaaa gatttgaaca acaagcgcgg atgaatattc aaaccgcgct
     3121 tgagggttta gctaattaaa ttaaactaac tcaatcgcca ttgcagtagc ttcaccacca
     3181 ccaatacata aagaagcaac accacgtttg ccaccgtatt gttttaaagc gtgaatcaaa
     3241 cttaaaatga tacgagaacc agtagagcca actggatgac ctaaagcaca agcaccacca
     3301 tgtacgttaa ctttgtcatg gggaatgttg tgtttaatca tcgcagccat tgtcaccatt
     3361 gcaaaagctt cgttgatttc aaataagtca acatcagcaa cgttccaacc tgtcttagca
     3421 aataattttt ccatcgcgcc aattggagca atggtaaatt cgcttggatg ttgagattgt
     3481 gtgctgtggg caacaatacg tgctaaaggt tgtaaaccac gtgctttagc agtgctttcg
     3541 cgcattaata ctaatgcaga tgcaccgtca gaaatagagc tggcgttagc ggcagtgatt
     3601 gtaccgtctt tagcaaatgc tggttttaag ctagggattt tttcgatttt agcattacca
     3661 ggttgttcat caatggcaac taaggtatca ccattacgac ctttaacagg cacaccaacg
     3721 atttcggctt caaaacggcc ttcagcaatc gctttttggg cgcgctttaa agaggcaatc
     3781 gcaaaatcgt ccatttgttc acgggttaac tctaattttg tcgccatttc ttgagcaaaa
     3841 gaacccatta aacgacctgt ttcagcatct tctaaaccgt ctaagaacat atggtcttta
     3901 atgtcaccat gacccatacg ataaccacca cgagctttgg ttaagacata gggtgcattg
     3961 gtcatagact ccataccacc tgcaacagca atatcaacac tgccagccac taataaatcg
     4021 tgagcaaaca tggtggcttt cataccagaa ccacaaagtt tgttaatggt ggttgcacct
     4081 gttgcggctg gcaaacctgc ttttaacatt gcttgacggg cagggccttg ttttaaacca
     4141 gcaggcaaca cacaacccat aatcacttct tgcacatctt ctggttgaat accagcacga
     4201 gcaatcgctt ctttaatagc aaccgcgcca agttctgggg cagtcacgct agataaagaa
     4261 ccttggaaac cacccattgg ggtacgagca ccatttacga taacaatagg atcatggctc
     4321 atttttaaac tccacttata tatgagatgt gctaaacaaa cctgacgatt atacggtatt
     4381 tctgtgcgga tgtaggtgtt tgagtggctt tttatttgac ttaatggttc aaaatacact
     4441 gtagtaaaaa tactacatca attgccatat tttgcgatag aaatgcaatc ttggaatcag
     4501 cataatacac acatggcgca ggatgatgca aaggatagca aaaaaggaca ggacataagc
     4561 cataggatgt agaggaagag gctaagatag ccaaacgatc ataagctttc aggatgatag
     4621 caaatgatta gcgtagcagc aaatggatac gcacagggag ta
//
LOCUS       DAOQMB010000164         4654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8740_length_4654_cov_4.208089, whole genome shotgun sequence.
ACCESSION   DAOQMB010000164 DAOQMB010000000
VERSION     DAOQMB010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8740_length_4654_cov_4.208089"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..750
                     /locus_tag="PLY05_06585"
     CDS             139..750
                     /locus_tag="PLY05_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC03008.1"
                     /translation="MTKIPTKHLPADKRRAITVDAVVALAAQQNPHDITTSAIAKHMG
                     VSQGALFKHFPSKDDILKAVMEWVAERLLSRIDAATLHAPTPIAAIEAIFLANIEFIV
                     EHPGAPRMMLGELQRNGQTAPKQLVHTIITRYRKRLYRLLEDAKNKGEVDQQLDIETA
                     ITMFIGTIQGLVVQSLITDDIQRIHHEAPRVLAIYLRGIGSNK"
     gene            747..1898
                     /locus_tag="PLY05_06590"
     CDS             747..1898
                     /locus_tag="PLY05_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC03009.1"
                     /translation="MMLSASKRRMMSIIFVISVLVALLIYVALRTGPLAPVIVTATTV
                     QSRSITPAVFGIGTIEARYTYKVGAITTSKLKRLDVNVGDAVHEKQVIGLMDSVDIDE
                     RIHAQQANRQRIEAIMRESTARQRYAQNQARHYEALFLTHAVSEDALRAKNLELEVAN
                     ATAAATKADFSRINADIEALNLLKANFQLIASADGIVTARTAEVGTTLLAGQPIIEFI
                     DPQTLWINVRFDQVNISGLRAKLPAHIVLRSHKNQILTGQILRIEPKADTITEEAMAK
                     VIFDSPPSPLPLIGELAEVTIHLPPLPAALVVPNAAIQRQGEQVGVWKIIENKPHFVA
                     VTLGEATLDGDVQIMKGLTLGDHIVVYSEKALLPLHELRIVKSIPEALK"
     gene            1895..3097
                     /locus_tag="PLY05_06595"
     CDS             1895..3097
                     /locus_tag="PLY05_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC03010.1"
                     /translation="MISLAGRDIFHAWGKFVFTGLGLGLLIGVTLIMAGVYRGMVDDG
                     KALLDNSGADLWVVQKDTLGPYAESSSLYGDLYRTVLAVSGVAEASNVTYLTMQVHKN
                     NQDIRSMVVGIMPHAHAGAVGWPPYLIAGRHLTRGHYEAVADIATHLKLGDIIKIRRN
                     HYTVVGLTRRMVSSSGDPMVFIPLKDAQEAQFLKDNQAIWQSRHRTESNTPFSPNTVS
                     EADKLTDSQFSNHLVNAILVRIKPHTSADEVAESIRRWKHLTVYTRVQMENILIGKLI
                     ATSAKQISMFLVILAVVSAAIVAFIIYTLTMDKIREIAVLKLIGTRNRTIAAMILQQA
                     LALGVIGFAVGKITATFAAPLFPKYVLLIPTDSLIGFFAVLTICALASVIAIRIALQV
                     DPAEAIGG"
     gene            3102..3812
                     /locus_tag="PLY05_06600"
     CDS             3102..3812
                     /locus_tag="PLY05_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC03011.1"
                     /translation="MNNGICIEGLKKRYGSGDSAFDALKDVNMQVKAGEVVGLIGPSG
                     SGKSTLLKCLGAVITPTAGRMTLGDEVIYDNGWKVSDLPALRRDKIGFIFQQPYLIPF
                     LNVIDNVALLPMLAGYSNEESRAKALSLLTALEVQHRAYAMPSLLSGGEQQRVAIARG
                     LVNRPPVILADEPTAALDSKRAMAVIRILNEMAAQFETAIIVVTHDEKIIPTFKRIYH
                     IRDGVTHEEQGEGRHIED"
     gene            3946..4278
                     /locus_tag="PLY05_06605"
     CDS             3946..4278
                     /locus_tag="PLY05_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC03012.1"
                     /translation="MQELDNNMQLTNHAMTRGDWDVVAKTALLIAEHPEPPFSEKLRI
                     MMFFGRDISRLKQLDGQTHQAAKNLATEAKTGNRQNIISSFNTLEQSCTNCHQQFKNA
                     FIKHFYKP"
     gene            4466..>4654
                     /locus_tag="PLY05_06610"
     CDS             4466..>4654
                     /locus_tag="PLY05_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC03013.1"
                     /translation="MRNYPINNLETLALELKTIRRRLKLSQQALAERTLLSRRTITNA
                     ETAHSVGLLEFTRMANALG"
BASE COUNT         1346 a         1067 c         1007 g         1234 t
ORIGIN      
        1 ccttgtgcat aagacaggcc tttgtgtgtt tgttttagaa gagtgaacca aaacgagtga
       61 agcttttact tacttggctt gacaagatta aaacctcagc taagatagta attactcact
      121 tactttatgg cgtattttgt gactaaaatt ccaaccaaac atttacctgc tgataagcgg
      181 cgagcaatca ccgttgatgc tgttgtagca cttgccgcac aacagaaccc gcatgacatt
      241 actacttccg ctattgccaa gcatatgggt gtttcacaag gcgcattgtt taaacacttt
      301 ccaagcaagg atgacatttt aaaagctgtt atggaatggg ttgctgaacg attgttatcg
      361 cgcattgatg ccgcaacact tcatgcaccc actcccattg ctgcgattga agcgatcttt
      421 ctcgctaata ttgaatttat cgtcgagcat ccaggggcac cacgcatgat gttaggcgaa
      481 ctacaacgta atggccaaac agcccctaag cagttggtac acacgattat tacgcgctac
      541 cgcaaacgtc tttatcgcct acttgaagac gctaaaaata aaggtgaagt cgatcagcaa
      601 cttgacatcg aaacggcgat tacgatgttt attggcacga ttcaaggctt agtggttcaa
      661 tcattaatca ccgatgatat acagcgcatt catcatgaag cacctcgagt attagcgatt
      721 tatttgcgcg gtattgggag taataaatga tgctatctgc aagcaaacgc cgaatgatga
      781 gtattatttt tgtcatttcg gtattagtcg cactcttaat atatgtcgcc ttacgcacag
      841 gccctcttgc gcccgttatc gtgaccgcca cgacggtgca atctcgctct attacacctg
      901 cggtatttgg tattggaacg atagaagccc gttataccta taaagtaggt gcaataacga
      961 ccagtaaact caaacgctta gatgtcaatg tcggtgatgc cgtccatgaa aaacaagtca
     1021 ttggattgat ggatagcgtt gatattgatg agcgcattca cgcccaacaa gccaatcgtc
     1081 aacgcataga agccatcatg cgtgaatcaa cagcgaggca acgttatgca caaaaccaag
     1141 cgcgtcacta cgaagcattg tttctcacgc atgcggtaag tgaggatgct cttagagcca
     1201 aaaatttaga acttgaggtt gccaatgcaa cagccgcagc caccaaagca gatttttcac
     1261 gtatcaacgc ggatattgag gcgttaaact tattaaaggc aaactttcaa ttaatcgcga
     1321 gtgccgatgg tattgtgacg gctcgtaccg ccgaagtggg gacaacgctc ttagctggcc
     1381 aacccatcat tgagtttatt gacccgcaaa ccttatggat caatgtccga ttcgaccaag
     1441 ttaacatctc gggtctgcgc gccaaactac ccgcgcatat tgtcctacgc tcccacaaaa
     1501 accagatttt aacaggccaa attttacgga ttgaacccaa agccgacacc atcaccgaag
     1561 aagcgatggc aaaagttatt tttgactccc ctccttctcc cttgccgtta atcggtgagt
     1621 tggccgaagt gaccatccat ttacccccac ttcctgcggc acttgtggtg cctaatgccg
     1681 caatacaacg ccaaggcgaa caagtgggag tctggaaaat catagaaaac aaaccccatt
     1741 ttgttgctgt gacattgggc gaagcgacgc tcgatggcga cgtacaaatc atgaaaggct
     1801 taacgcttgg tgaccacatt gtggtttata gcgaaaaagc cctactccct cttcatgagc
     1861 tgcgtatcgt taaaagtatt ccagaggcct taaaatgatc agtttggctg ggcgtgatat
     1921 ttttcacgca tggggaaaat ttgtttttac gggcttagga ctgggtttgt taatcggtgt
     1981 cacgctgatt atggcaggag tctatcgcgg catggtcgat gatggcaaag cactgctcga
     2041 taatagtggt gcagatttgt gggtggtaca aaaagatacg cttggaccct atgcggaatc
     2101 ctctagcctc tatggtgatt tatatcgtac cgtgttagct gtttcggggg ttgcagaagc
     2161 cagcaatgtc acttacttga ccatgcaagt acataaaaat aatcaagata ttcgttcaat
     2221 ggttgttggc attatgcctc atgctcatgc tggcgcagtc ggctggccgc cttacttaat
     2281 cgcgggacga catctcacgc gtggccatta tgaagccgta gccgacattg ccacgcactt
     2341 aaaactcggt gacatcatca aaattcgccg taatcactat acggtcgtcg gtttaacccg
     2401 tcgaatggtg tcttctagtg gtgacccgat ggtatttatt ccgcttaaag atgctcaaga
     2461 agcacagttt ttaaaagaca atcaggccat ttggcaaagt cgccatcgca ccgaatctaa
     2521 cacgccattt agtcctaata ccgtctctga agccgacaaa ctcacggata gtcaatttag
     2581 caatcatttg gtcaatgcca ttcttgtacg tattaaacct catacctccg ccgatgaggt
     2641 ggcagagtct attcgtcgat ggaaacatct cactgtttat acccgcgtac aaatggaaaa
     2701 tattctgata ggcaagttga ttgccacgtc agcaaaacaa attagtatgt ttttggttat
     2761 tttagcggtg gtcagtgccg ccattgtggc ctttattatt tacaccttaa ccatggataa
     2821 aatccgtgaa attgccgtcc tcaaactgat tggcacacgc aaccgaacca ttgcggccat
     2881 gattttacaa caagcactgg ccttaggcgt tattggcttt gcggttggca aaattaccgc
     2941 tacgtttgcc gcgccactat ttcctaaata cgtgctgtta atccccacag attcgctgat
     3001 tggcttcttt gctgtgttaa caatttgcgc cttagccagt gtcattgcga ttcgtattgc
     3061 cctacaagtt gaccctgccg aagcgattgg aggctaaacg gatgaacaac ggtatttgta
     3121 ttgaaggtct caaaaaacgc tacggcagtg gcgactctgc ctttgatgcc ttaaaagatg
     3181 tcaatatgca ggtaaaagcg ggcgaagtgg tggggttgat tggcccttct ggctcaggaa
     3241 aaagtacgct actcaaatgt ttaggcgcgg tgattacgcc aacagcagga cgaatgacgt
     3301 taggggatga agttatttat gacaacggct ggaaagtcag cgacttaccc gcgctaagac
     3361 gcgataaaat aggctttatt tttcaacagc cttatttaat tccattttta aatgtcattg
     3421 ataacgtcgc attgttaccc atgttggcgg gctattctaa tgaagaatca cgcgccaaag
     3481 ccttgtcgtt attgacggca ttagaggtac aacatcgtgc ttatgccatg ccttcgctac
     3541 tatcaggagg cgaacaacaa cgcgttgcta ttgcccgagg attagtcaat cgtccgcctg
     3601 tgattcttgc cgatgaaccc actgccgcac tcgatagtaa acgcgcaatg gcggtgatac
     3661 gcatactcaa tgaaatggcc gctcagtttg aaaccgccat tattgttgta acgcatgatg
     3721 aaaaaattat tccgacgttt aaacgaattt accatatccg tgatggtgta acccatgaag
     3781 aacaaggtga aggccgtcat attgaggatt aaaacaagag actattttat gtttatgcag
     3841 atgctatcaa aactagcgac cgtcttgcta ttgacaatat cgaccatagc aattacttgg
     3901 gcagatgaga caccacccaa aaacgcaatg gttttaagca aaataatgca agaactcgat
     3961 aacaatatgc aattaaccaa ccatgcaatg actcgtggag actgggacgt tgttgccaaa
     4021 acagcattac ttatcgccga gcatccagaa ccgcctttta gtgaaaaact acgcattatg
     4081 atgttttttg gccgagatat tagtcgatta aaacaattgg acggtcaaac acaccaagcg
     4141 gccaaaaatc tagccactga ggctaaaacg gggaatcgtc aaaatatcat aagctctttt
     4201 aacacgctcg aacaaagctg tacgaactgc caccaacagt ttaaaaatgc ttttattaag
     4261 cacttttata agccatagtt gtagcggctt gcatttgaaa aaatgctcat gcctgcattt
     4321 catcgcatca ggaaagcatt aactgcaaat gaaaaaacgg ccctaatcgg taactccccc
     4381 cacattaaca atcgcatcaa cacaaaacac acaatgcgcg ttataatgct caaatataaa
     4441 taatgtaaaa tatatgagta taaagatgcg caactatcct atcaataatt tagaaacact
     4501 ggcattagag ttaaaaacta ttcgacgtag gctaaaacta agccaacaag ccctagccga
     4561 gcggactttg ttatccagac gcacgattac caatgccgaa acagcacatt cagtcggttt
     4621 gttagagttt acacgcatgg ccaatgcctt aggt
//
LOCUS       DAOQMB010000165         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8784_length_4638_cov_15.985817, whole genome shotgun sequence.
ACCESSION   DAOQMB010000165 DAOQMB010000000
VERSION     DAOQMB010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8784_length_4638_cov_15.985817"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /gene="ccoP"
                     /locus_tag="PLY05_06615"
     CDS             <1..785
                     /gene="ccoP"
                     /locus_tag="PLY05_06615"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HNC03014.1"
                     /translation="IKEYDNPMPKWWQWLYWGGVAFFIFYVLAYPALGSYKGFLGWTS
                     VGEHDKDKAAYDAKYAPIFKAYAAKPIEELAHDAAAMKIAGRLYVNNCAACHGADARG
                     AKGYPNLTDNDWLYGGEPAQIVETITNGRLAVEGGSKMPAWGETLGEQGVKEVAAYVL
                     TLSDRKLSEADQKLAEAGQARFAVCAGCHGADGRGMHAVGAPNLTDTIWLYGGSQKAV
                     EETIRHGRNGAMPAWKDRLGEEKVHLLAAYVYSLSHGGAAKH"
     gene            953..2374
                     /gene="ccoG"
                     /locus_tag="PLY05_06620"
     CDS             953..2374
                     /gene="ccoG"
                     /locus_tag="PLY05_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HNC03015.1"
                     /translation="MSNKIPTQDQSPNPVQIHSPKLMHTDKAKIFPRSIRGFYQNIRH
                     GVMGLIFFIYVALAWVTWDGRQAVLFDLEHRQFFIFGWTFAPQDFFLLSWLLIIAAFS
                     LFVVTVFAGRVFCGYVCPQTTWTSLYLWIEKITEGERNARIKLDKAPMSVNKFVRRAV
                     KHFLWLLIAFATGFVFVGYFTPVRSLAEDFMTGNLGGWGYVFILLFTVLTYLNAGWMR
                     EQVCFYMCPYGRFQSVMFDKDTLIVSYDAERGESRGARKKTADPKAAGLGDCIDCQLC
                     VQVCPTGIDIRDGLQLECIQCAACIDACDSIMDQMGYQRGLVRYTTEHILKEKTGYHW
                     LRPRLIGYSAMVLIMMGLFVYTLAGRVPLSIDAIRDRNQLYRENSEGLIENVYTLKVM
                     NKSQQTQQYHLSLVEGQEHIQLMPIGDFSLAAGEVSNLPVTLQADPMDLKQTNTKISF
                     KVEAVADKNVTAQVESRFLSPSN"
     gene            2494..3009
                     /locus_tag="PLY05_06625"
     CDS             2494..3009
                     /locus_tag="PLY05_06625"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNC03016.1"
                     /translation="MTDTVDNKPWYKHPWLWFLIAIPVVSVTLSFTMLFVALNHKDSE
                     VEGDWRKNKKAIQENFNRENYASALSINALLSVNGNQLTVKISSPYPLDKDALPKQLN
                     VIFSHPTHENKDLRLVLNQQNNGDYQTTIGQEISGRYYLDVNTTVWRLKETIEMPLVA
                     PLTVKPLALDI"
     gene            3014..>4638
                     /locus_tag="PLY05_06630"
     CDS             3014..>4638
                     /locus_tag="PLY05_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNC03017.1"
                     /translation="MSESCFHCGEPLPATPYRLTILGKEQPLCCLGCYAVAETIVQNG
                     LESYYLERTVISPTAAVPEALEQLTGYDHPDTQKQFIHREQDLACAELSVEGVSCAAC
                     AWLIEKRLTQEQSVKQVSLNLSNHRLRLLWDDKKTPLSHLLVDLEKLGYKARPFRTDT
                     HASLLKKESRQLLMRLGVAGLGSMQAMMYAGSLYVAEDMTLDHRDFLRWVSLLITTPL
                     FFYSAYPFYRSAWFSLKARTLNMDVPVTIALILTFAASIYNTVIGAPDVYYDSVTMFI
                     FFLLAGRFVETQARRRASETANDLIPVIPRLSHRLLANGQTEDVPVTDLQIGEHILVK
                     AGETIPCDGQIIQGQSSVSEALLTGEALPISKQQGDTVVSGSQNHQHPLTIEVSQIAQ
                     ASTLATIHQLINRALAEKPELAEQADKFAHWFILSILLLSLVVYGAWSVVNPNFALLA
                     TIAVLVATCPCALSLATPVALTAATHRLAQQGFLVTRGHVINRLAQATHIIFDKTGTL
                     TTGELQLVKQQTLQGNDQEALLIAAALEQYSEHP"
BASE COUNT         1283 a          902 c          958 g         1495 t
ORIGIN      
        1 gcattaaaga atatgacaac cctatgccca aatggtggca gtggctctat tggggcggtg
       61 tcgcattctt tatattttat gttttagctt atccagcttt aggttcttac aaaggctttt
      121 taggttggac atcagttggt gaacacgaca aagacaaagc tgcatatgac gcaaaatatg
      181 caccaatctt taaagcttat gctgctaaac cgattgaaga attagctcat gatgccgctg
      241 caatgaaaat tgcaggtcgt ttatatgtca acaactgtgc agcttgtcac ggtgctgacg
      301 cacgtggtgc taaaggttat ccaaacttaa ccgataacga ttggttatat ggtggtgagc
      361 cagctcaaat cgtggaaacg attactaatg gtcgtttagc ggttgaaggt ggtagcaaga
      421 tgcctgcttg gggtgaaact ttaggcgaac aaggcgtcaa agaagttgct gcttatgtgt
      481 taactttatc tgaccgtaaa ttgtctgagg ctgatcaaaa attagctgaa gcaggtcaag
      541 ctcgttttgc agtatgtgct ggctgtcatg gtgctgatgg tcgtggtatg catgctgtag
      601 gcgcaccaaa cctaaccgat actatttggt tatatggtgg cagccaaaaa gctgttgaag
      661 aaaccattcg tcatggccgt aacggtgcta tgcctgcatg gaaagaccgt ttaggtgaag
      721 aaaaagtaca tttgctagct gcatatgttt actctttatc tcatggcggt gctgctaagc
      781 actaagcttt gttaggactt acgctttgcg taagtcctaa tgactgttat caatttttct
      841 tatcaattaa tttttatata tcagttatgc tctcatttag taattatatg tttatttgtt
      901 tgctttagca cataaagata taattattgc cacagaggta gccagtctca ttatgagtaa
      961 taaaattcct actcaagacc agtcccctaa tcctgttcaa attcacagtc ctaaattaat
     1021 gcacaccgac aaggcaaaga tttttcctcg ttcaattcgt ggtttttatc aaaatattcg
     1081 tcatggggtg atgggtttaa ttttctttat ttatgtcgct ttagcttggg tgacatggga
     1141 tggacgacaa gcagtattat ttgatttaga acatcgccaa ttttttattt ttggttggac
     1201 gtttgcccca caagactttt ttttattatc ttggctatta attattgctg cttttagcct
     1261 atttgtggtc actgtttttg caggacgtgt attttgtggt tatgtttgcc cacaaacaac
     1321 gtggacaagt ttgtatttgt ggattgaaaa aattaccgaa ggcgaacgta atgcacgtat
     1381 caagcttgat aaagctccga tgtcggtcaa taaatttgtg cgccgtgcag ttaaacactt
     1441 tttatggtta ttaattgctt ttgccacagg ttttgtgttt gttggctatt ttacgcctgt
     1501 gcgtagttta gccgaagatt ttatgacagg aaatttaggt ggttggggct atgtgtttat
     1561 tctacttttt actgtcttaa cctacctaaa tgcaggctgg atgcgcgaac aagtttgttt
     1621 ttatatgtgt ccttatggtc gttttcaaag tgtcatgttt gataaagaca ccttaattgt
     1681 gtcttatgat gcagagcgtg gcgaatctcg tggcgcacga aaaaagactg ccgaccccaa
     1741 agccgcaggc ttaggtgact gtattgactg ccaattatgt gttcaggttt gtccaacagg
     1801 cattgatatt cgtgatggtt tacaattgga gtgcattcaa tgtgcagctt gtattgatgc
     1861 ctgtgacagc attatggatc aaatgggcta tcaacgtggt ttagttcgtt ataccacgga
     1921 gcatattctc aaagaaaaaa caggttatca ttggttacga ccacgcctca ttggttatag
     1981 tgctatggta ttaattatga tgggcttatt tgtttatact ttggcaggcc gtgtacccct
     2041 aagtattgat gccattcgtg accgtaacca actttatcgc gaaaacagcg aaggcttgat
     2101 cgagaatgtc tatacgctaa aagtcatgaa taaatctcaa caaactcaac aataccacct
     2161 gtcattagtt gaaggtcaag aacatattca attaatgcca attggtgatt ttagtttggc
     2221 tgctggcgaa gtgtctaact tgcctgtcac gcttcaagca gaccctatgg atctcaaaca
     2281 aactaatacc aaaatctcct ttaaagttga agcagttgct gataaaaacg tcaccgcaca
     2341 agtggaaagc cgctttttat cgcctagtaa ctaagactgt aagacttatg ctgataagtc
     2401 ctccctattt catctttgga ctgatacaac gcccgttgcc tcagtcctac tctgactcaa
     2461 tacggatgat tcacgctttt gaggtaatca ccaatgactg atactgttga caacaaacct
     2521 tggtacaaac atccgtggtt atggtttttg attgcaatac ctgttgtttc tgtcacttta
     2581 agttttacca tgctctttgt tgctttaaat cataaagact ctgaggttga aggcgattgg
     2641 cgcaaaaata aaaaagccat tcaagaaaat tttaatcgtg aaaattatgc tagtgccttg
     2701 agcattaacg cgttactaag tgttaatggc aatcaattga ccgttaaaat ttctagtcca
     2761 tatcctttag ataaagatgc gctgcctaaa cagttaaatg ttattttttc tcatcctact
     2821 catgagaata aagatctgcg tttagtacta aatcaacaaa acaatggcga ctatcaaacg
     2881 actatcggtc aagaaatttc tggtcgttat tatttagacg tgaataccac tgtttggcga
     2941 ttgaaagaaa ctatcgaaat gcctttagtt gctcccttaa cagttaagcc tttagcctta
     3001 gatatttaag tctatgagtg aatcctgttt tcattgtggt gagcctttgc ccgccacacc
     3061 ttatcgttta acgattttag gtaaagaaca acctttatgt tgtttaggtt gttatgcggt
     3121 tgccgaaacg attgtgcaaa atggtctaga aagttattac cttgaacgta cagttattag
     3181 ccctactgcc gccgtacctg aagcactaga acaattaaca ggttatgatc atccagatac
     3241 acaaaaacaa tttattcacc gtgaacaaga cttagcttgt gccgaattaa gtgttgaagg
     3301 agtgtcttgt gccgcctgtg cgtggttgat tgaaaaacgc ttaacccaag aacaatctgt
     3361 taaacaagtt agcctcaatc tgagcaatca tcgcttgcgg ttgttgtggg atgacaaaaa
     3421 aacaccttta agtcatttac tagtagactt agaaaaactg ggttataaag cccgaccttt
     3481 tcgtaccgat actcatgcct ctttattaaa aaaagaatcc cgacaattat taatgcgatt
     3541 aggggtagca ggtttaggct ctatgcaagc catgatgtat gcaggtagcc tgtatgttgc
     3601 cgaagacatg accttagacc atcgtgattt tttacgttgg gtctctttat taattacgac
     3661 tcccttattt ttttattcgg cttatccttt ttatcgttcg gcttggtttt cgctcaaggc
     3721 acgcacactt aatatggatg tgcctgtaac aattgcattg atattaacgt ttgcagcgag
     3781 tatttataac accgtcattg gtgcacctga tgtctattat gactcagtga ccatgtttat
     3841 tttcttttta ttagcaggtc gctttgttga aactcaagca cggcgcagag ccagcgagac
     3901 agccaacgat ttaatccctg ttattccgcg tttaagtcat cgcttattag cgaacggtca
     3961 aactgaagat gtacctgtca ctgacttaca aattggtgaa catattttag ttaaagcagg
     4021 tgaaactatt ccctgtgatg gtcaaattat tcaaggccaa agtagtgttt cggaagcttt
     4081 attaacaggt gaagccttac cgattagcaa acaacaaggc gataccgtgg tcagtggtag
     4141 ccaaaatcat caacatcctt taaccattga agtcagccaa atagcacaag ctagcacttt
     4201 agccactatt catcagttaa ttaatcgtgc tttagccgaa aaacccgaac tcgccgaaca
     4261 agctgataaa tttgcccatt ggtttatttt aagcatttta ctgctatctc tagtggttta
     4321 tggcgcgtgg tcggtggtta atcctaactt tgccttatta gccaccattg ccgttttagt
     4381 agccacttgt ccatgtgctt tatcattggc cacacctgtt gccttgactg ctgccacaca
     4441 tcgtttagcg cagcaaggct ttttggtcac tcgtggtcat gtgattaatc gtttagccca
     4501 agccacacac attatttttg ataaaacagg cacgttaacc acaggtgagt tgcaactggt
     4561 taaacaacaa acgctgcaag gtaatgacca agaagcttta ttgattgctg ctgcccttga
     4621 acaatattct gaacatcc
//
LOCUS       DAOQMB010000166         4604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8892_length_4604_cov_15.583425, whole genome shotgun sequence.
ACCESSION   DAOQMB010000166 DAOQMB010000000
VERSION     DAOQMB010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8892_length_4604_cov_15.583425"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1367
                     /locus_tag="PLY05_06635"
     CDS             <1..1367
                     /locus_tag="PLY05_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03018.1"
                     /translation="NSSHKQGTVTVDMPQITPEQALQYAPDDSSLKAAKKLAPANQWQ
                     QLHLHQTDDQLAILWGEIRGSSLYQSAILLSDKFVFHCNCPSFKRPCKHSLALLICYA
                     QSQNLFKTQQATPDWLNKHIEKHQTSQAKKQEKAAAPAKVVDEAAQAKRAAAREQKVN
                     AATAELQQWLVDLLRTGLAQAKTLSYSHFERIKSRLVDGQASGLVSMVEDLQSALAHQ
                     DWQQRAAVQIGKIQLILAAYQRIEQLPAPLQADVRSIIGWTTAQEQVLANSEAAQDWL
                     IVGNRELEGVNGLRYRRQWLWHKPQNKAALVLQFAAGFQPLAPAWPNKHLFSAEVAWY
                     PSAWPQRIVIKSQTPLQLASTTTTGFSHIEQALEHQAQALAHNPFLAIFPMLLQQVVP
                     HYDNGQLWLIDKQQQALPLQVLNKTWPLLALSAGHPICVFGEWDGYVLTGLSAWTENE
                     VISL"
     gene            1364..2824
                     /locus_tag="PLY05_06640"
     CDS             1364..2824
                     /locus_tag="PLY05_06640"
                     /inference="COORDINATES: protein motif:HMM:NF039502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5691 domain-containing protein"
                     /protein_id="HNC03019.1"
                     /translation="MSAWQQLQVAALLGTQKNSPSPQWPTELAPLMSQFSENSLLNQL
                     AALSAYQRCAFSAITMANPTPSSPETLAASTRQQQKWLSYLLSYDGLDYLLDWLNLAS
                     KKQVAFPAAQLPELLDIGYKHKKLRLAICQVAGQRGAWLAERNTDWQWLQGGQISLEN
                     EQLSDYWHTASAASRELLFERLRLANPAQARDFLQQVWREEAATTRKLLLEKFSANLS
                     VEDHDFLESCLDDRSRVVNEQTVELLASLQGSLLQQRLQHTLSQHLTLKKGLIHKSLQ
                     IEAIEQVTDVLERDGLNPKAADIKNGLGEKAQWLRDVIAGVSLDWLSHHYELQAEAFI
                     HAALKTDWAEALLIGLSTAAIRQKHNVWLKALLDVDSKKLALPRFRLFDALSISDKES
                     WLLAHAGQSKKELERGSVFIQFFYQSQPWLWTHYFTIAVMDVYQQQLKLQQSNNQLGH
                     YLARYGDAQLAQKANQYPTQIINTWLMRMEIEQAFS"
     gene            2924..4003
                     /locus_tag="PLY05_06645"
     CDS             2924..4003
                     /locus_tag="PLY05_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004880886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03020.1"
                     /translation="MTNVLRQRAEHQFLHELDALAAQDHKTKPPQWKLSPQAVVTYLL
                     GGKLDNGVEITPKYIGNRRLMEIAVATLATDRALLLLGVPGTAKSWVSEHLAAAISGD
                     SSLLVQGTAGTSEEALRYGWNYAMLLAKGPSAEALVPSPMLNAMKDGKIARVEELTRI
                     PADVQDTLITLLSEKTLPVPELGSDFQAIKGFNVIATANNRDKGVNELSSALKRRFNT
                     VILPVPDNLEEEISIVQNRVASLGKVLELPDSPPAIEQIRRVVMVFRELRSGVTEDKK
                     AQLKMPSGTLSTAEAISVMSNGWALASHFGDGLMTAHDVAAGLMGAVLKDPVQDRVPW
                     QEYLETVMKERDGWKDLYRACKELD"
     gene            4021..>4604
                     /gene="rhuM"
                     /locus_tag="PLY05_06650"
     CDS             4021..>4604
                     /gene="rhuM"
                     /locus_tag="PLY05_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HNC03021.1"
                     /translation="MSDIAIYTNSSGHVEVTIEQDSVWLNLNQLSLLFQRDKSVISRH
                     IRNIFIEHELERVSVVANFAITANDDKNYQVEHFNLDVIISIGYRVKSQEGVRFRQWA
                     TRLLKQHLTQGYTLNQQRLQENSHALEAALRLVKRTAESQALTLETGRGLVDIITRYT
                     HTFLWLQRYDEGLLSEPTGIIGGQLAPLDVARHA"
BASE COUNT         1392 a          965 c          987 g         1260 t
ORIGIN      
        1 aaaattcttc ccataaacaa ggaactgtca ctgtggatat gccacaaatt acgcccgaac
       61 aagccttaca atacgcgcct gatgatagct cgcttaaagc cgcaaaaaaa ctagcacctg
      121 ctaatcaatg gcaacaactt catctgcatc aaaccgatga ccagttagcg attttatggg
      181 gagaaattag aggcagttcc ttatatcaaa gtgctatttt attatctgat aaatttgttt
      241 ttcattgtaa ttgtccaagc tttaaacgcc cttgtaagca cagtttagcc ttactgattt
      301 gttatgctca aagccaaaat ttgtttaaaa cacaacaagc tacccctgat tggctaaaca
      361 aacatattga aaaacatcaa actagccaag ccaaaaaaca agagaaagca gctgctcctg
      421 ctaaagtcgt tgatgaggca gcccaagcta aacgtgctgc cgctcgtgaa caaaaagtca
      481 atgcagctac ggctgaattg caacaatggt tggttgattt attacgaact ggtttagcac
      541 aagccaaaac cctgagttat agccattttg aacgcatcaa aagccgttta gtggatggcc
      601 aagccagtgg tttagtgagc atggttgaag atttacaatc tgctcttgcc catcaagatt
      661 ggcaacaacg tgctgccgtc caaataggca aaattcaatt aattttggca gcttatcaac
      721 gcattgagca attacccgct ccgttacaag cagatgttcg tagcatcatt ggttggacta
      781 ccgctcaaga gcaagtatta gccaatagcg aagcggctca agactggcta attgtgggta
      841 atcgtgagtt ggaaggtgtg aatggtttgc gttatcgccg acaatggttg tggcataagc
      901 cacaaaataa ggcagcatta gtgcttcagt ttgcggctgg ttttcaaccg ctagcccctg
      961 catggccaaa caaacatctg tttagtgccg aagtagcttg gtatcccagt gcatggccac
     1021 aacggattgt cattaaaagc caaacgccac tgcaattggc cagcacaaca accacaggtt
     1081 ttagccatat tgagcaagcc ttagaacatc aagcacaagc cctcgcccac aatccctttt
     1141 tagcgatttt tccaatgtta ttacaacaag ttgtgccgca ttatgataat ggtcaactct
     1201 ggttaattga taaacaacaa caagctctac ccctccaagt tttaaacaaa acatggccat
     1261 tattagcgtt gtcagcagga catccgattt gtgtgtttgg cgaatgggat ggttatgtgt
     1321 taacaggact cagtgcatgg actgaaaacg aggtgatttc tttatgagcg catggcaaca
     1381 actgcaagtc gcggccttat taggcacaca aaaaaatagc ccaagccctc aatggccaac
     1441 agaacttgcg ccattgatga gtcaattcag cgaaaatagc ttactgaatc aattagctgc
     1501 gctgagtgct tatcaacgtt gtgcttttag tgccattact atggccaatc ccacgccatc
     1561 atctcccgaa accttagcag caagcactag acaacaacaa aaatggctta gttatttatt
     1621 aagctatgat gggctagatt atttattaga ttggttaaac ctcgccagca aaaaacaagt
     1681 cgcttttcct gccgctcaat tacccgaact attagacatt ggttataaac acaaaaaact
     1741 gcgtttagcg atttgccaag ttgctggtca acgtggtgct tggttggctg aacgtaatac
     1801 cgattggcaa tggttacaag gggggcaaat ctcgcttgag aatgagcaat taagtgacta
     1861 ttggcatacc gcctctgcgg ccagtcgaga gttattattt gagcgtttac gtttggctaa
     1921 ccctgctcaa gcacgcgatt ttttacaaca agtttggcgt gaagaagcgg ctaccacgcg
     1981 caaattatta ctagaaaaat tttcggctaa tttaagcgtt gaagatcacg attttttaga
     2041 aagttgtttg gatgatcgta gtcgggttgt taacgagcaa accgttgaat tattagcgag
     2101 tttacaaggc tctttattac aacagcgttt acaacacaca cttagccaac atctgacact
     2161 caaaaaaggc ttaattcata aaagcttgca gattgaagct attgaacaag tgactgatgt
     2221 gttagagcgt gatgggctaa atccaaaagc agccgatatt aaaaatggct tgggtgaaaa
     2281 agcccaatgg ttgcgagatg tgattgctgg ggtgtcttta gattggctgt ctcaccatta
     2341 tgagttacaa gcagaagcat tcattcatgc ggcattaaaa actgactggg cagaagcctt
     2401 attaattggt ttgagcacag ccgccattcg ccaaaaacac aacgtttggc tcaaagcctt
     2461 actcgatgtt gatagcaaaa aattggcttt gccacgtttt cgtttatttg atgctctatc
     2521 aatctctgat aaagagtctt ggttattggc acatgcagga caaagcaaaa aagaactcga
     2581 aagaggctcg gtatttattc aattttttta tcaaagccaa ccgtggttat ggacgcatta
     2641 ttttactatt gcggtgatgg atgtttatca acaacaacta aaactacaac aatcgaataa
     2701 tcaattaggc cattatttag cacgttatgg cgatgcgcaa ttagcacaaa aagccaatca
     2761 atatcctact caaattatta atacatggtt gatgcggatg gagattgagc aggcgttttc
     2821 ttagcagcct gacgacattt attgtcgtta tatcactcag gctactagcc aaaaccaaca
     2881 caacaataca tcattcaaca atcaaatcta aggaaaagcc aacatgacta acgtactccg
     2941 ccaacgtgcc gaacaccaat ttttacacga actcgatgcc cttgctgctc aagaccataa
     3001 aactaaaccg ccgcaatgga aactatctcc tcaagctgtc gtcacgtatt tattgggtgg
     3061 aaaactcgat aatggcgttg aaattacccc caaatacatt ggtaatcgtc gtttaatgga
     3121 aattgccgtt gctaccttag ccactgaccg tgctttattg ttattgggag tacccggtac
     3181 agcaaaatct tgggtgtctg agcatttggc cgccgcgatt agtggtgata gtagtttgtt
     3241 agtgcaaggt acggctggta ctagtgaaga agcattacgc tatggttgga attacgccat
     3301 gttattggcc aaagggccta gtgcggaagc tttagtgcct agcccgatgc ttaacgcgat
     3361 gaaggacgga aaaattgccc gtgttgaaga gttaacgcgt attcctgctg atgtccaaga
     3421 taccttaatt accctgttat ccgaaaaaac cttacctgtt cccgaattgg gcagcgactt
     3481 tcaagcgatt aaaggcttta acgtgattgc gactgccaac aatcgtgaca aaggcgttaa
     3541 cgaattatcc tctgcgctta aacgccgttt taataccgtg attttgcctg tgccagataa
     3601 tttagaagaa gaaattagca ttgtgcaaaa tcgtgttgct tcgttgggca aagtgttaga
     3661 acttccagac agtccacctg ccattgagca aattcgtcgt gtggtgatgg tgtttagaga
     3721 gttacgttct ggcgtaacag aagacaaaaa agcgcaactt aaaatgccca gtggcacact
     3781 cagtaccgct gaagcaatct cggtgatgag taatggttgg gcattagcct cgcattttgg
     3841 tgatggctta atgaccgctc atgacgtggc agcagggtta atgggggcag tgttaaaaga
     3901 ccctgtgcaa gatcgtgtac cttggcaaga atatttagaa acagtcatga aagaacgtga
     3961 cggctggaag gatttataca gagcgtgtaa agaattggat taatttttag gaagaatcgt
     4021 atgagtgata ttgccatcta taccaacagc tctggccatg ttgaagtcac cattgagcaa
     4081 gacagtgttt ggcttaacct caatcaatta agcctgttat ttcaacgaga taaatctgtt
     4141 atttccagac atattcgtaa tatatttatt gagcatgaat tagagcgggt ttcagttgtt
     4201 gcaaattttg caataactgc taatgatgac aaaaattatc aagttgaaca ttttaatctt
     4261 gatgtgatta tttctattgg ctatcgtgtc aaatcacaag aaggtgtacg ttttcgccag
     4321 tgggcaacac gtttattaaa acaacactta actcaaggct acacactcaa tcagcaacga
     4381 ttacaagaaa acagtcatgc cttagaagca gcactacgct tggtaaagcg cactgcggaa
     4441 agccaagcct taacccttga aactggtcgg ggtctggtcg atattattac ccgttacacc
     4501 cacacttttt tatggctaca gcgttacgat gaaggtttat tgagcgaacc tacaggcata
     4561 atcggtggtc aacttgcacc cttagatgtc gctcgtcatg ccat
//
LOCUS       DAOQMB010000167         4593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_8920_length_4593_cov_15.838695, whole genome shotgun sequence.
ACCESSION   DAOQMB010000167 DAOQMB010000000
VERSION     DAOQMB010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8920_length_4593_cov_15.838695"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..96)
                     /locus_tag="PLY05_06655"
     tRNA            complement(10..96)
                     /locus_tag="PLY05_06655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(60..62),aa:Leu,seq:caa)
     gene            199..1236
                     /gene="queA"
                     /locus_tag="PLY05_06660"
     CDS             199..1236
                     /gene="queA"
                     /locus_tag="PLY05_06660"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017396016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HNC03022.1"
                     /translation="MQLSDFSYHLPDELIARYPLEQRSASRLLYLSQQGVQHKQVVDL
                     PTLIKPHDLLVFNDTRVLAARLYGQKSSGGKVEVLVERIISDDTALVHLRASNSPKAG
                     AQLLFANGQVQAQMLERVEDLFKLQFSQPIRGVLAQYGEMPLPPYLNRQVEQDDSERY
                     QTVYSDPNKQNSVAAPTAGLHFDDTLLANLKAAGANFAYVTLHVGAGTFRPVKTEDIR
                     DHQMHSEWLNVPEQTVQAINETKAKGGRVIAIGTTSVRALESAALYNDGQLKAWSGET
                     QIFIYPSYQWKVVEALFTNFHLPESTLLMLVCAFAGKEAVMNAYAQAVEQKYRFFSYG
                     DAMFVERSKIS"
     gene            1505..1859
                     /gene="rnpB"
                     /locus_tag="PLY05_06665"
     ncRNA           1505..1859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLY05_06665"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2030..>2148
                     /gene="mraW"
                     /locus_tag="PLY05_06670"
     CDS             2030..>2148
                     /gene="mraW"
                     /locus_tag="PLY05_06670"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNC03023.1"
                     /translation="MNNPFVHQSVLLHETVDAIVGDTNGIYIDATFGRGGHSRL"
     assembly_gap    2149..2248
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2249..2572
                     /gene="mraW"
                     /locus_tag="PLY05_06675"
     CDS             <2249..2572
                     /gene="mraW"
                     /locus_tag="PLY05_06675"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HNC03024.1"
                     /translation="ATRCFQAIRIAVNQELDDIKHFLPQAIEALKPQGKLAVISFHSL
                     EDRLIKQFLQKQARGDDLPRDLPVRSSELNPQVKLLGKTAASHQEVTANPRSRSAYLR
                     MAQKL"
     gene            2582..2881
                     /gene="ftsL"
                     /locus_tag="PLY05_06680"
     CDS             2582..2881
                     /gene="ftsL"
                     /locus_tag="PLY05_06680"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HNC03025.1"
                     /translation="MWWDVFKTSFKPNFNVIVAMLVIASLVGSSVAIALSVQHWRESF
                     QQLQDLKEEKKALEIEWGQLLLEQNSWGSYARIEQMAQEQQMQPTTVNSVVMVQP"
     gene            2878..>4593
                     /locus_tag="PLY05_06685"
     CDS             2878..>4593
                     /locus_tag="PLY05_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC03026.1"
                     /translation="MKFKLPSFKLNENEVYAGRHLLLQVVLAFIFLGISARAFYLHII
                     DRDFLRRQGDARMMRTEAIPAHRGMIMDRNGVPLAVSTPVTSIWVNPKEIYELANPPK
                     EDNPKKQKQPVSFPYEQVASAIGLDPVELRRKITNNPKKEFMYLKRHLAPDVAKVILD
                     RDFPAIYGLNEYRRYYPQGESTAHIIGFTDLDDKGKEGVELAFENQLAGAAGAQQIIQ
                     DRKGHKIKDVALLRPAKAGSDIYLSFDARIQYIAHRELARAVQEHEADAGYLVAIDVK
                     TGEIIAIVNQPAYNPNNRSKLIPDQLRNRSITDMFEPGSTMKIFTVMAALESGKYTTS
                     SLFNTNPGRYRVYNKIVSDHHNYGVIDLKTLITKSSNIASVQIAESLPKETLPMMHRR
                     FGFGQVTESGFPGESKGILQPPKRWGPVEIATMSYGYGVTVTALQLARAYAIVANHGV
                     SHPTSLTRINHASEGTPLISEKIVDQLFPMMESVVSSDGTALKAALPGYRVAGKTGTA
                     HKAQAGGYAKDAYMSLFAGFAPVSDPRIAMVVVMDGAKKGGYYGGTVSAPVFSRVMGE
                     ALRLMN"
BASE COUNT         1279 a          897 c         1024 g         1293 t
ORIGIN      
        1 ttggaatctt ggtgcctaga gccggactcg aaccggcacg cccgcaaagg cggtggattt
       61 tgagtccact gcgtctaccg attccgccac ctaggcaagg taggcttgcg attataatca
      121 ccaaatcatt agcgtcaagc tttttaataa aaacttgcta taatatgcgc cctatttgtc
      181 taattcttgc ccaaatccat gcaactgtct gattttagct atcatttacc cgatgaatta
      241 attgcccgtt atcctttgga acaacgctca gcatcccgtt tattgtattt gtcacaacag
      301 ggtgtacaac ataaacaagt cgttgattta ccaactctaa ttaagcccca tgatttattg
      361 gtgtttaatg atactcgtgt gttggcggcg cgtttatatg gccaaaaaag ttcaggtggc
      421 aaagttgaag tattggtgga gcgtattatt agtgacgata ccgctttagt gcatttacgt
      481 gcgagcaatt cacccaaagc aggtgcacaa ctgttgtttg cgaatggcca agtgcaagca
      541 cagatgctcg aaagggtaga ggacttattt aagttacagt ttagtcagcc gatccgtggt
      601 gttttggcac aatatggtga aatgccattg cctccctatc tcaatcgtca agtagaacaa
      661 gatgatagcg agcgttatca aacggtttat agcgacccca ataaacaaaa ctctgtggca
      721 gcacccaccg caggtttgca ttttgatgac accttattag ccaatttaaa agcagcaggt
      781 gcaaattttg cttatgtgac cttgcatgtg ggggcaggta catttagacc tgtgaaaaca
      841 gaggatattc gtgaccatca aatgcacagt gagtggttaa atgttcctga acaaaccgta
      901 caagcgatta acgaaactaa agctaaaggt gggcgggtga ttgcgattgg tacaaccagc
      961 gttcgtgctc tagaaagtgc tgctttgtac aatgacggtc agttaaaggc ttggtctggt
     1021 gaaacacaaa tttttattta tcccagttat caatggaaag tggtagaggc attgtttacc
     1081 aattttcatt tgcctgaatc caccttattg atgttggtct gtgcatttgc aggcaaagaa
     1141 gctgtgatga atgcttatgc acaagccgtt gagcaaaaat atcgcttttt tagttatggc
     1201 gatgcgatgt ttgtggaacg ttctaagatt tcttgaactg aattaaccca actcttaaag
     1261 gacaaattca atatttagct tgtgtgcgtt ttttaatcaa agttcgcggt tgacttgggc
     1321 ataaagaccc atttgatcgt ttatcccaat gatggagaat gggattttcc tgtcaaaaca
     1381 cgcacagtct ataacctcga agttgcggac tatcataact attttgttgg tgaaggtggc
     1441 atttgggtaa aatcaaatta aatttcttgc ctattataca aagcctgtta tccttgcgcc
     1501 cctgagagtc ggccagacgg tcgccgcctg caaaggggga ggaaagtctg ggcttcaaag
     1561 ggcaaagtgc taggtaacgc ctaggcagtg tgaactgacg gaaagtgcaa cagagagctg
     1621 aaccgccgat gtttggtgtg caaacaccaa gcaggtaagg gtgaaaaggt gcggtaagag
     1681 cgcaccgcgc aactggcaac aggagcggca gggtaaaccc cacttgaagc aagaccaaat
     1741 aggcatccat tggcgtggcc cgcgctggat gcgggtaggt tgcttgagcg tataggtgac
     1801 tatacgccta gaggaatgac cgtcctcgac agaacccagc ttatcggctg actctcattt
     1861 ttttacttag ctacttgacg ttaactcaag ctctccctat agtttgcagt ttgcgctgca
     1921 tcgtcttatt ctattcctat caacggctta aacgatttac gcgtcaaaaa gtaaactgtg
     1981 tggcaattgc ctaatcctaa ttattccccc tctctaaact ttaagtctta tgaacaatcc
     2041 ttttgtgcat caatcagtgt tattgcatga gacagtcgat gcgattgtcg gtgacacaaa
     2101 tggtatttat atcgatgcca cctttggtcg tggtggccat agtcgtctnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc aacacggtgt tttcaggcga taagaatagc
     2281 tgttaatcaa gaattagatg atattaaaca ctttttacca caagcgattg aggcattaaa
     2341 accacaaggt aaattggcgg tgattagttt tcattcttta gaagaccgtt tgattaaaca
     2401 atttttgcaa aaacaagcgc gtggtgatga tttgcctcgt gatttgcccg ttcgtagcag
     2461 tgagttaaat ccacaagtga agcttttagg caaaacggcg gcgagtcatc aagaagttac
     2521 agctaatcct cgttcacgca gtgcttattt acgcatggca caaaaattat aagagtcagt
     2581 tatgtggtgg gatgttttta aaaccagttt taaacccaac tttaacgtta ttgtggcaat
     2641 gcttgtgatt gcttctctag tcgggtcttc agtcgctatt gctttatcgg tacagcattg
     2701 gcgtgagtct tttcagcaat tacaagattt aaaagaagaa aagaaagccc ttgaaataga
     2761 atgggggcaa ttgttgttag agcaaaattc atggggaagt tatgctcgaa ttgaacaaat
     2821 ggcgcaagag caacaaatgc aacccacaac cgttaatagt gtggtaatgg tgcaaccatg
     2881 aaatttaaac tacctagctt taaattaaat gaaaatgaag tctatgcagg gcggcatttg
     2941 ttgttgcaag tggtgttagc gtttattttc ttgggtattt cagctcgcgc attttattta
     3001 cacatcattg atcgtgattt tttacgtcgt caaggtgacg ctcggatgat gcgtaccgaa
     3061 gctattcctg cgcatcgtgg catgattatg gatcgtaatg gtgtgccttt agcagtaagc
     3121 acacctgtca cttctatttg ggtcaaccct aaagaaattt atgagcttgc taacccaccc
     3181 aaagaagata atcccaaaaa acaaaagcag cctgtcagtt ttccttatga gcaagtggcc
     3241 agtgctattg gtttagaccc tgtcgaatta cgtcgtaaaa ttactaataa tcccaaaaaa
     3301 gaatttatgt atttaaaacg tcatttagct cctgatgttg ctaaagttat tttagatcga
     3361 gactttcctg ctatttatgg cttaaatgaa tatcggcgat attatccgca aggtgagtca
     3421 acggcacaca taattggttt taccgattta gatgataaag gtaaagaagg ggtagagttg
     3481 gcttttgaaa atcaattagc aggtgccgca ggcgcacagc aaattattca agaccgcaaa
     3541 ggccataaaa ttaaagatgt tgccttgctt cgtcccgcta aagcagggag cgatatttat
     3601 ttgagttttg atgcgcgtat tcaatatatc gctcatcgag agttggcgcg tgctgtacaa
     3661 gagcatgaag ccgacgcagg ctatttggtg gcgatagatg taaaaacagg tgaaatcatc
     3721 gccattgtta atcaacctgc ttacaatcct aataatcgta gtaaattaat cccagaccaa
     3781 ctgcgtaatc gttctattac cgatatgttt gaaccgggtt cgaccatgaa aatttttact
     3841 gtcatggcgg ccttagaaag tggaaaatat accacgtcaa gtttatttaa taccaatccg
     3901 ggacgttatc gcgtttataa taaaatcgta tcagaccatc ataactatgg cgtgattgat
     3961 ttaaaaacgt taattaccaa atcaagtaac attgcctcgg tacaaattgc agagtcttta
     4021 cccaaagaaa ccttacccat gatgcaccgc cgttttggct ttggtcaagt gacagaaagt
     4081 ggctttccgg gtgaaagtaa aggcatttta caaccgccaa aacgttgggg gcctgtcgaa
     4141 attgccacca tgtcttatgg ttatggggtg acggtaacag cactgcaatt agcaagagct
     4201 tatgccattg tggctaatca tggcgtatca caccccacct cattaacgcg tatcaatcat
     4261 gcatcagaag gaacaccatt gatttcggaa aaaattgttg accaattatt tcccatgatg
     4321 gaatccgtcg tctcatcaga cggtacagcc ttaaaagctg ctttacccgg ttatcgtgtc
     4381 gcaggtaaaa caggtacagc gcataaagca caagcgggcg gctatgccaa agatgcttat
     4441 atgtctttat ttgcagggtt tgcgcctgtc agtgacccgc gtattgcaat ggttgttgtc
     4501 atggacggtg ctaaaaaggg cggatattat ggcggtacag tctctgcacc tgttttttca
     4561 cgcgtgatgg gtgaggcgtt acgattaatg aat
//
LOCUS       DAOQMB010000168         4560 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9043_length_4560_cov_4.657936, whole genome shotgun sequence.
ACCESSION   DAOQMB010000168 DAOQMB010000000
VERSION     DAOQMB010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9043_length_4560_cov_4.657936"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLY05_06690"
     CDS             <1..678
                     /locus_tag="PLY05_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC03027.1"
                     /translation="MLGPIGWFATGLWLVIDLAGPAYRKTVPAVVHIAMLRQMLINRI
                     TIGVVGDGSSGKDSMIKSVFGIDTGNINPVAGSTKDAKIYKLGDSDAIQLVNYPGFND
                     IRQDVNDQTHDLLQYTDVFVMVVDITRGISQTEIEILKKLQTFQHPILICLNKIDLAR
                     PKDKPVLLQTAQERLKGFDLIETSFDPDPRLTDTKEGCFEVHAWICNELTKKGKETQH
                     IPKAVFS"
     gene            complement(726..1508)
                     /locus_tag="PLY05_06695"
     CDS             complement(726..1508)
                     /locus_tag="PLY05_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNC03028.1"
                     /translation="MNFLCRALIEKSGHDNGFENSLLNTEQQVLLGSARHTLQLSITQ
                     TNEGLAVQFSEPQFITLLAVEFPDFKQQQQALIVLENELGFLLKAAFKLAVLTPTEIT
                     KNYENQVQAELAKLPSNGTEVERMVRQRVGQQSFRQALMTYWGGACALTGVAIPDVLR
                     ASHAKPWAECVSDAERLDVYNGFLLNANLDALFDRFLISFDEQGYLLISSTLSQVDLL
                     SLGIKSGLKLRWINALHQPYLAFHRARFNQSSPLINRTYPVG"
     gene            complement(1505..2230)
                     /locus_tag="PLY05_06700"
     CDS             complement(1505..2230)
                     /locus_tag="PLY05_06700"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNC03029.1"
                     /translation="MAREKATMTDFFRFPHTPHIVWLGKDSPRDDKVLSPIEAQQLLA
                     HQVIVEEKLDGANLGFSVSANGELRAQNRGQYLLQPYVGQFEKLENWLKPRADSLFDA
                     LGENLMLFGEWCAAQHSLDYQTLPDWFLVFDVYDKQQQQFWSVTRRNQLAKQLDLAVV
                     PEVFRGRITLAQLKERIEQKQSLYRDGHLEGLVIRQDSQLWCEKRAKLVRADFTQAIT
                     DHWRKKRLQWNQLDFMTAPRKAI"
     gene            complement(2206..2721)
                     /locus_tag="PLY05_06705"
     CDS             complement(2206..2721)
                     /locus_tag="PLY05_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03030.1"
                     /translation="MKARYVVDTNVLIAASAIQNAKLDVTPDDPVLCQQVFEWLSQFE
                     QADSYLVLDGEGKIHEEYEKKLGFNDYGLQVLMLKYSQCQVDMVDVLYDDDGYAFLEE
                     PLASAIYDRSDRKIVAAALEGLKLGECEIANASDADWYDWTEALEQQGLLVHQIIPDW
                     SKQKWQEKKQR"
     gene            complement(2718..4037)
                     /locus_tag="PLY05_06710"
     CDS             complement(2718..4037)
                     /locus_tag="PLY05_06710"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HNC03031.1"
                     /translation="MFTMLQLKNFKAWRDSGEVQLAPVTMLLGANSTGKSSLLQSLLL
                     LKQTVNSPDRSIHLNLGGDEVNDYFNFGDFDDVLTQGASPREFGIGLGIKRIRPSHLT
                     SIESTRFIQTAKLLTTYKKITGGAPAIKDLLLESGDNKYRLLRKNKGAYAVFLGEGTE
                     QLGESRLYAPERSIAFSADAIQLLASHGGKAEDISLAIRRELESISYLGPLRRKPERD
                     YVWNKSKPGELGIDGGGVINAILSSALTKNKENDADNIIDGVSKWLARMGVAERLDVR
                     QLGRSTRYELVVIKDGIEANLRDVGIGISQVLPVLTLAYFAPQGSTILLEEPEIHLHP
                     LAQSVLAELFVEVSKERQVQFIVETHSEHIFRRMQTLMAKKAVTSDDCQMYFVEREGA
                     DAKLCKLDVDAYGRVKNWPNKFFGDALGETKEQARLMFERQKQERQE"
     gene            complement(4127..>4560)
                     /locus_tag="PLY05_06715"
     CDS             complement(4127..>4560)
                     /locus_tag="PLY05_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03032.1"
                     /translation="FDFAARQKIGGITLNFFIAKQFPVLPPSAYIQADLDYIVPRVLE
                     LIYTAHDLKPFAEDLGFYGEPFKFDPDRRAILRAELDAYYATLYGLSRDELRYILDPA
                     DVMGADYPSETFRVFKNNDIKAYGEYRTGRLVLEAWDRLHT"
BASE COUNT         1346 a         1045 c          843 g         1326 t
ORIGIN      
        1 atgcttggcc ctattggctg gtttgcaact ggactctggc ttgtgattga tctcgctggc
       61 cctgcctacc gaaagaccgt tcctgcggtg gtccatatag cgatgttgcg gcaaatgctg
      121 attaaccgta tcaccattgg cgtcgttggc gatggctctt ccggcaaaga ctccatgatc
      181 aaatctgtat ttggtataga tacaggtaac attaaccctg ttgctggctc cactaaagac
      241 gccaagattt ataagcttgg tgactctgat gcaatccaac tagtgaacta tccaggcttt
      301 aatgatattc gtcaggatgt taatgaccaa acacatgacc tccttcagta cactgatgtt
      361 tttgttatgg tcgtggacat cacgcgtgga atttctcaaa ctgaaattga aattcttaaa
      421 aaactacaaa cctttcaaca ccccatattg atctgcctta ataagataga tttagcccgc
      481 cctaaagaca aacctgtttt attacagacg gcacaagaac gactaaaagg ttttgacctc
      541 attgagacat catttgatcc agatcctagg ctcacagaca caaaagaagg gtgttttgag
      601 gttcatgctt ggatttgcaa tgagcttacc aagaaaggga aagaaactca gcatatcccc
      661 aaagcagtat ttagctaata ctaaaactac tataagctac ataaagtagc tcccaaataa
      721 ggtacctagc ctacgggata ggtacgatta attagagggc tagattgatt aaatctagct
      781 ctatgaaaag ctaaataggg ttgatgtaac gcattgatcc atctaagctt cagtcccgat
      841 tttataccaa gcgacaataa atctacttgt gataaagttg atgaaatcaa taagtaacct
      901 tgctcatcaa aactaatcaa gaatcgatca aacaaagcat ctaaattagc attcagtaaa
      961 aaaccattat aaacgtctaa tctttcggca tcggatacac attcagccca aggcttagca
     1021 tgactagcgc gtaatacgtc tggaatagca acacctgtta gagcacacgc tccaccccaa
     1081 taagtcatta aagcttggcg aaagctctgt tggccaacac gttggcgtac catacgctca
     1141 acttctgtgc cattgctagg taacttggcc aactcagctt gcacttgatt ttcgtagttt
     1201 ttcgtaattt cagtcggtgt taaaacagct aatttaaatg cagcttttaa caaaaaacca
     1261 agctcatttt ctagcacaat taaagcttgt tgctgttgct taaaatcagg aaattcgaca
     1321 gccaatagcg tgataaattg tggttcagaa aactgaacag ccagcccttc gtttgtttgg
     1381 gtaatgctaa gctgtagcgt atgacgcgca gagccaagca atacttgctg ttctgtattt
     1441 aacaggctat tttcaaaacc attatcatgg cctgattttt ctattaaggc acgacataaa
     1501 aaattcatat tgctttccgt ggagcagtca tgaagtcgag ctgattccat tgcaatcgct
     1561 ttttacgcca atggtcggtg atggcttgcg tgaaatctgc tctgactaat ttggcgcgtt
     1621 tttcacacca aagctgtgag tcttgacgaa tgactaagcc ttcaagatgc ccatctcgat
     1681 acaagctttg tttttgctct attcgctctt taagctgcgc taaggtaata cgcccacgaa
     1741 aaacctctgg tacaacagct aagtctaatt gtttagcgag ttgattacgg cgcgtgacac
     1801 tccaaaactg ttgttgttgt ttgtcataga catcaaagac caaaaaccaa tctggcaatg
     1861 tttgataatc gagggagtgt tgtgctgcac accattcacc aaacagcata agattttcgc
     1921 ctaaagcatc aaacaaacta tctgcacgag gttttaacca attctctaac ttttcaaatt
     1981 gccctacata gggttgcaat aagtattggc cacgattttg agcgcgtaat tcaccgttag
     2041 cactgaccga aaagcctaaa ttagcaccgt ccagtttttc ttctacaatc acttggtgcg
     2101 ctaatagctg ttgtgcttct attggcgata acactttgtc atcacgcggc gagtcttttc
     2161 ccaaccatac aatgtggggg gtatgaggga atctaaaaaa atcagtcatc gttgcttttt
     2221 ctcttgccat ttttgttttg accaatcggg gataatttga tgaacaagca acccctgctg
     2281 ctcaagtgct tctgtccagt cataccaatc ggcatcagac gcattagcta tttcacattc
     2341 acctaacttt agcccttcta aagcggcagc aactattttt cggtcagaac ggtcatagat
     2401 agcagaggct aaaggttctt ccaagaaggc gtagccatcg tcatcataaa gcacatccac
     2461 catatctact tggcattgac tatatttaag catcaatact tgcagtccat aatcattaaa
     2521 acctaatttc ttttcatatt cttcgtgaat tttgccttca ccatctaata ctaaataact
     2581 gtctgcctgc tcaaattgac tcaaccattc aaacacttgc tgacaaagta cagggtcatc
     2641 tggcgttaca tctagctttg cattttgtat cgcactagcg gcaatcaaaa cgttagtatc
     2701 aacaacataa cgcgccttca ttcctgacgc tcctgctttt gacgctcaaa cataagccgt
     2761 gcttgctctt ttgtttcgcc caaggcatca ccaaaaaact tgtttggcca gtttttgacg
     2821 cgcccataag catcaacgtc cagcttacat aacttagcat ccgctccctc tctttcaaca
     2881 aaatacattt gacaatcatc gctagtgacc gctttttttg ccattaaggt ttgcatacga
     2941 cgaaatatgt gttctgagtg ggtctcaaca ataaattgca cttgtcgctc tttactcacc
     3001 tcaacaaaca attctgctaa tactgattgt gctaacggat gtaaatgtat ttcaggctct
     3061 tctaataaaa tggttgagcc ttggggtgca aaataagcca acgttaatac aggcaatact
     3121 tgagaaatac caatgccgac atcgcgcaaa ttagcttcta taccatcttt aataacgact
     3181 aattcataac gggtagaacg ccctaattgg cgtacatcta aacgctctgc aacgcccatt
     3241 cttgctaacc actttgatac gccatcaata atattatcgg catcgttttc tttgtttttt
     3301 gttaatgcac tggataaaat ggcattaata acaccaccgc catctatgcc taattcgcca
     3361 ggcttgcttt tattccacac atagtcgcgc tctggcttac ggcgtaatgg ccctaaatag
     3421 ctgatacttt caagctcacg acgaatagcc aaactaatgt cttctgcttt gcctccatga
     3481 cttgccaata actgaatggc atccgccgaa aaagcaatag aacgttctgg cgcatataaa
     3541 cgactttcgc ctaattgctc agtgccctcc cccaaaaaga cagcataagc ccctttgttt
     3601 ttacgcagga gccgatattt attatcgcct gactctaata acaaatcctt tatagctggc
     3661 gcaccaccag ttattttttt atatgtagtg agaagcttag ctgtttgaat aaaacgagta
     3721 ctttcaatac ttgttaaatg cgatggacga atccgcttaa tgcctaaacc aataccaaac
     3781 tctcttggcg atgcaccttg cgtaagtaca tcgtcaaagt caccaaagtt aaaatagtca
     3841 tttacttcgt caccacctaa gttaagatgg atgctacggt ctggtgaatt aacggtctgc
     3901 tttaacaata ataaactttg caataaagat gatttacctg tggaatttgc gcccaatagc
     3961 atggtgacag gcgcaagttg tacttcgcca ctatcgcgcc atgctttgaa gttttttagc
     4021 tgtaacattg taaacataat taaactttcc taaattatca gccactcacc tgacaaaatc
     4081 acgtctataa cagcccactc aacttattat gggcttagtt taagtttcag gtgtgcagtc
     4141 tatcccacgc ctctaaaacc aatctacctg ttcgatattc accataagct ttgatgtcgt
     4201 tgtttttaaa gactctaaac gtttccgagg gataatctgc acccatgaca tcggcagggt
     4261 ctaaaatgta gcgtagttca tcacgagaca acccgtacag ggtcgcgtag taagcatcta
     4321 actcagcacg aagtatggca cggcggtcgg ggtcaaattt aaaaggctca ccataaaagc
     4381 ccaaatcttc ggcaaagggt tttaagtcat gggcggtgta aatcaactct aaaacacgcg
     4441 gcacgatgta atctaaatct gcttgtatat aagccgaagg aggaagtaca ggaaattgtt
     4501 tggcaataaa aaagtttaat gtaattcccc ctattttttg tcgagcagca aaatcaaaca
//
LOCUS       DAOQMB010000169         4559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9047_length_4559_cov_10.796181, whole genome shotgun sequence.
ACCESSION   DAOQMB010000169 DAOQMB010000000
VERSION     DAOQMB010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9047_length_4559_cov_10.796181"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4559)
                     /locus_tag="PLY05_06720"
     CDS             complement(<1..>4559)
                     /locus_tag="PLY05_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter-associated beta strand
                     repeat-containing protein"
                     /protein_id="HNC03033.1"
                     /translation="GNGRLVKTGGGTLTLNNTANDYNLGTVVDSTGVETAIVGNTVIN
                     GGVLAISNNDQLGIAAQTGKLSDDKDLYKWQLGNVLVDGGTLQTTQSLESDRHIYVGA
                     AGATVNVDGAANTTVLTGAIKNDGAVGGSLVKEGAGTLKIDLAFKNYLKSDDSFGITA
                     NDSIGTITVKEGVLEVFDTGKDLGGAANLVLAGGEFRVSNSSNIGFVTLQGTGGTIDT
                     TGATITTGEITGSGGLKKQGSGTLVLTGLNNQNFTGDTTISEGKVQIDSAQGLGTTGQ
                     LVLDGGSLQVVGNTEVTLDKKAVITSNNGTVDNVSSDVTLSGVVSGAGQLVKSGAGKF
                     SLTNEKNSFEGGVRIKDGTLEVSTDGSLGKANTQVLIDGATLAIAKDANLSRQIVLTE
                     KGGAVSVAGTNTATLSGTLSLDAGVLSADFTKSGTGTLNLTKDNTNFKGNTIIQEGTL
                     GITGKDNLAQGKLFLDGGNLRTNASLNFDKEIIINSTGGIDTSLDASGNKTEVTVISK
                     IQGNGGLVKTGEGTLTLSADNAYAGGTQVKGGTLVVNQDSNLGLQATDITLNGGTLQL
                     GAADAVSFKKGDAASRLLKVGQEGGGLDIGAFDLTIETDISGDGTLTQTGTAKKDANG
                     NIVKDQYGQTVYGTLTLKGNNTVSGITVESGTLKIDANQNLGADAAQLTLKDGTTLDT
                     TDANTKLDLAHGITVGDGINGATLRQDEDLSIRGLTGTDKATLRVDKGAKTLAFNGDS
                     SAFAGNLLVQGNIEVKQNLGTGNVEFNQGVESNAPDSNLHITGNATFDNQFKLNGQTT
                     INADKDTKSVFNKAVKNADYNTDPGNDLTVQYRTGSLIKTGEGEISLNAENLYSGNTD
                     IQQGTVTVANNKALGIGNVKLQNGTTLQSGADVTLANKVELVSGNVTLSTPQINPLTT
                     PQNTSLTLTDIVSGAASLTKTGTGSLILNGNNTYAGETIIKAGKVAINNAAALGVNTN
                     VELNANATLSVNVALEANQQPSTITLNKNIALKGASGSQVTLSATGANVVLDGMLSND
                     ANTVGIIKEGMGTLELSGTNTNYKGKTTIKEGNLAISEAKNLGGKVVVELAGGGLTLL
                     KDMTIGGGASTDAIYITGARGAINTATSSTLEVAFKGQGLFVKEGEGQLTLSGTNTEF
                     SGGVSIDKGTLTITKDDSLGKKDTNVILNGGTLQTDGVTTLNRDVQLNQDSTIKNIGA
                     STVGLNRLQGNYAVTLEGGGFNLKAKIDETTDPDTVVNVNQHNGTILKNTNVIIGNDA
                     DLGKSTGKLNFAGGATLDIQGVQGQVVQDESDAIRNISMRNMGVEGTGNVLDVNEFNV
                     TLGTLNSTLGKQTTEANTGITKQGTGNLILNKDNKDYKGTLTISEGAVFVSKDNALGV
                     GKAVFETVKLTTLAATNSTNNIGLNGALSIDGQTFDNTLNGVISGTGSLVKVGGRVDG
                     VDMGTGTLTLTGDNNYRGGTRIDAGKVLLNADSALGKGKAIFAKDTELTLDVNKKVLL
                     DTGIEQGFSVANAIEVKGEVDIHSG"
BASE COUNT         1248 a         1086 c          705 g         1520 t
ORIGIN      
        1 ctgccactat gaatgtcaac ttcaccttta acctcaatcg cattggcaac cgagaagcct
       61 tgctcaatac ccgtatcaag taatactttt ttattaacat ctaaggttaa ttcggtgtct
      121 ttggcaaaga tggctttacc ttttcctaac gcactatcgg catttaatag tactttacct
      181 gcgtcaatac gagtgccgcc tctatagttg ttgtctcccg ttagcgttaa tgtgcctgta
      241 cccatatcga caccatcaac acgaccaccc actttaacaa gactacctgt accactaata
      301 acgccattta aggtgttatc aaaggtttgg ccatcaatgc ttaatgcacc atttaaaccg
      361 atattattag tcgagttagt cgcagctaat gtggtcagtt taactgtttc aaaaacagcc
      421 ttacctacgc ctagcgcatt gtctttacta acaaaaacag caccttcact aatagttaag
      481 gtacctttat agtctttgtt atctttgttt agtattaagt tgccagtacc ttgcttggta
      541 atacctgtgt tcgcttcagt agtctgctta cctaatgttg agttaagcgt accgagagtt
      601 acattaaact cgttaacgtc taacacatta ccagtacctt caacacccat attgcgcata
      661 ctaatatttc gtatagcgtc actttcatct tgcacaactt gaccttgcac accttgtata
      721 tctaaggttg cgcctcctgc aaagttcaat ttacctgtgc tctttcctag gtcagcatca
      781 ttaccaataa tgacattagt attttttaag atggtgccat tatgttgatt aacattaaca
      841 acagtatcag ggtcggttgt ttcatctatt ttggctttta aattaaaacc tccaccttcc
      901 aacgtaaccg cataattccc ttgcaaacga tttaagccaa cagtactggc accaatattt
      961 ttaatagtgc tatcttggtt taactgcaca tcacgattta acgtagttac gccatcggtt
     1021 tgtaaggttc cgccatttaa aataacgttg gtgtcttttt tacctaaact atcatctttg
     1081 gtaatagtta aagtaccttt atcaatcgaa acaccgccac taaactccgt gtttgtgcct
     1141 gatagtgtta attgcccttc gccttcttta acaaacaaac cctggccttt aaaagccact
     1201 tctagggtac tgctagttgc ggtgtttatt gcaccgcgcg cacctgtaat ataaatagca
     1261 tcggtagatg ctccgccacc aatcgtcatg tcttttaata gagttaaacc accaccagcc
     1321 agctcaacca ctacctttcc acctaagttt ttggcctcac taatggctaa attaccttct
     1381 ttgatggttg ttttgccttt gtagttggtg ttagtaccac ttagctctaa agtacccatg
     1441 ccttctttaa taatacctac cgtattagca tcgttactta acataccgtc taaaacaaca
     1501 ttagcacctg tagcacttag tgttacttgc gaaccactag ccccctttaa ggcaatattt
     1561 ttatttaacg ttattgtgct aggttgttgg tttgcctcaa gtgcaacatt aacactcaat
     1621 gttgcattag cattaagctc aacattagta tttacgccta aagcagcagc attattaata
     1681 gcaaccttac ctgctttaat aatagtttcg cctgcgtaag tattattacc attaagtatt
     1741 aaactacctg tacctgtttt ggtaagagaa gcagcacccg aaacgatatc agtgagtgtt
     1801 aaagaggtat tttggggtgt ggttaatggg ttaatttgtg gcgtggacag tgtgacatta
     1861 ccactaacca gctcgacttt attagctaaa gtaacatcgg ctccactttg caaagttgtg
     1921 ccattttgta gttttacatt acctatgcct aatgctttat tattggcaac agtgaccgtg
     1981 ccttgttgta tatctgtatt gccactatac aagttttcgg catttaggct aatttcgcct
     2041 tcacctgttt taataaggct gcctgttctg tattgaaccg ttaaatcatt accagggtca
     2101 gtattatagt cggcattttt aacagcctta ttaaagaccg atttagtgtc tttatctgcg
     2161 ttaatggttg tttgaccatt taacttaaat tggttatcaa aagtcgcatt gcctgtaata
     2221 tgtaaattag aatcaggcgc attactttct accccctgat taaactctac gttacctgtg
     2281 cctaaatttt gtttaacttc aatgttgccc tgcaccaata aattaccagc aaaggcactg
     2341 ctatcaccat tgaaagcaag ggttttggca cctttatcaa cccttaaggt tgctttatct
     2401 gtacctgtta atccacgaat acttaggtct tcatcttgcc gtaatgtagc accattaatg
     2461 ccatcaccca cggtaatacc atgtgccaaa tcaagcttag tatttgcatc agtagtatct
     2521 aatgtagtgc catcttttag agtaagctgt gctgcgtctg cccctaagtt ttggttggca
     2581 tcaattttta atgtgccaga ctcaaccgta atgccactga cagtattatt tcctttaagc
     2641 gtcaatgtac catatacagt ttgaccatat tggtctttaa ctatgttacc atttgcatct
     2701 tttttagctg tgcctgtttg cgttaatgtg ccatcaccac taatatctgt ttctatggta
     2761 aggtcgaacg caccaatatc tagaccacca ccctcttgcc ctactttaag caagcgagat
     2821 gctgcatcac cctttttaaa acttaccgca tctgctgcac caagctgtaa tgtgccacca
     2881 tttaaggtaa tatccgttgc ttgcaaacct aaattactat cttgattaac aactaaagta
     2941 ccacctttaa cttgtgtacc gcctgcataa gcattgtcag cagataatgt taacgttcct
     3001 tcacctgttt taaccaaacc accattacct tgtattttac ttatcacagt gacttcggtt
     3061 ttattacccg aagcgtctaa tgaagtatca atcccacctg tgctattaat aataatttct
     3121 ttatcaaaat ttaaacttgc attagtacgc aagttaccac catctaaaaa taacttacct
     3181 tgtgctaaat tatctttacc tgtaattccc aaagttccct cttggataat ggtattacct
     3241 ttaaaattag tattatcttt ggttaagttt aatgtacccg tacctgattt agtgaaatcg
     3301 gcagataata cccctgcatc taatgacaat gtaccactta aggttgccgt atttgtgcct
     3361 gcgacagaaa ctgctcctcc tttttcggta aggacaattt gacgactcaa attagcatct
     3421 ttggcaatcg ctaacgttgc tccatcaatt aacacttgcg tatttgcttt acctaaagaa
     3481 ccatccgtac tcacctctaa tgtaccgtct ttaatacgta caccgccttc gaagctattc
     3541 ttttcattag tcaaagaaaa tttaccagca cccgacttca ctaattggcc agcaccactc
     3601 accacacctg ataaggtcac atcacttgac acattatcca cagtaccatt attactcgta
     3661 ataactgctt ttttatctaa tgtaacttct gtattaccca caacttgcaa agaaccacca
     3721 tccaatacta attggccagt agtacctaaa ccttgtgcag agtcaatttg cactttacct
     3781 tcactaatcg tggtatcacc tgtgaagttt tgattattta accctgttaa tactaatgta
     3841 ccactacctt gtttttttaa gcctcctgat ccagtaattt cccccgttgt aatcgtcgct
     3901 cctgttgtat ctatcgtacc acctgttcct tgtagtgtca cgaaaccaat attagaactg
     3961 ttgctaacac gaaattctcc accagctaac actaaattcg ctgctccgcc caagtcttta
     4021 cctgtatcaa agacttccaa aacaccttct tttaccgtga ttgtgccaat actatcgttc
     4081 gcggtaatgc caaaactatc atcacttttt aggtaatttt taaaagctaa atcaattttt
     4141 aacgtacccg ctccttcttt aactaaactt cctccaacag caccatcgtt tttaatcgcc
     4201 cctgttaaaa ctgtggtatt agctgcacca tctacattaa cggttgcacc tgccgcacct
     4261 acataaatat gacgatcaga ttctaaactt tgggttgttt gtaatgttcc accatctact
     4321 agcacattac ctaattgcca tttatacaag tctttatcat cgctcaactt tccagtttgt
     4381 gcggctatac ccagttggtc attattggag atggctaaca cccctccatt aataaccgta
     4441 ttgccaacaa tcgccgtttc gacaccagtc gaatcaacga ctgtgcctaa attataatca
     4501 ttagctgtat tattcagggt tagcgtacca ccacccgttt taactaaacg accattgcc
//
LOCUS       DAOQMB010000170         4519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9174_length_4519_cov_13.204973, whole genome shotgun sequence.
ACCESSION   DAOQMB010000170 DAOQMB010000000
VERSION     DAOQMB010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9174_length_4519_cov_13.204973"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLY05_06725"
     CDS             complement(<1..497)
                     /locus_tag="PLY05_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC03034.1"
                     /translation="MSHNQFDLLKTRRFLPFFSTQFLGAFNDNVFKQSLIILLAFNAA
                     SMTTLPASTLANLCAGLFILPFFLFSATAGQLADKYEKTKLIKIIKGAEIVIMLLAAI
                     GFHFQQLWLLLTALFLMGTHSTAFGPIKYAILPQALTKHELVGGNALVEMGTSLAILF
                     GSMLGG"
     gene            629..2416
                     /locus_tag="PLY05_06730"
     CDS             629..2416
                     /locus_tag="PLY05_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HNC03035.1"
                     /translation="MKRHWSPVFVLSLFVTVANAADNRFSELENYVSQFTKPDEKIKL
                     SIEGVSGEIKTNVEAYIGELTQEDLQQWRETQARIRKSSREALESVGYYQADIKIVKQ
                     DKAVLLTIQPNQAVIVSKINLQYQGEASNDIAFTALRETFPLKEGQVLHHGKYENAKA
                     LLQNMALERGYFEAKWLTHEVIVEQPSQQATINLVYDSGKRYKFGQTFFSGANQTQLP
                     VNLDLLVKFVPFKEGDDYEADKVIKLNKSLLDSRYFNQVKVRAESELAEEFFVPVNVS
                     LAAEKPNQLDIGAGYATDIGARLSSAWRRPLLNQRGHSIEVVSELSQVRQSLTMRYGI
                     PWTHPINDTLQISTGFKREDIDDSATTNNSVIGIERQKKRDSGWQTNESIRWSRESYH
                     QDNGEKGQSDLLLPSYSISRVRTKGSKTDPERGDRQYYQVEFASPDLLSDADLLSLRV
                     GWRWLNTFANRHQLALRADLGAIFSSNFDSVPLNMRFYAGGDQSVRGYDYKSLSPRDS
                     AGKVIGASNLMSLSAEYTFKLTSKWRLASFIDSGNAFDSASEPLKTGVGLGIRWISPV
                     GPVRLDVALPLDNTADNTPRIHFFMGPAL"
     gene            2416..>4519
                     /locus_tag="PLY05_06735"
     CDS             2416..>4519
                     /locus_tag="PLY05_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03036.1"
                     /translation="MLKKTSRFLWRSSCHLLVAVWLLLFLVWLVLVSLAHSTSGSRWL
                     LEKIAQQQKLIKYQVVSGNLIDGITLKNFRFTGKTFYLDAQELTLDILWIGLLNKELF
                     VEKLHGNGVQLVLHGQPNPHRTQLKHLKIPLRIVLSDGLLLNAKINKRGVITPIDRLA
                     LQQAQWQGEDLKFAELNVIHPQFSTLLQGQLAFKADYPLQAIGIVQAKFWQKQSVKPI
                     KIKVIGDLGNLGVNLVAKPVALSATLDVLEPSLDYVANVRWGYWQFPWLSAQNFVSRY
                     GQVNIRGNKKHLELSVNTDLQSNLLPRGEYRAFAETDWQHLKLLPLEARTILGGKLAL
                     NGQISWQNGIQWQLNTDWSNVDLAKKWPVINSYLPKLTGKLQTQGLASAQQSKLNIDA
                     QWANGEQWQIKQQNKDWFWHWQQQQQLQASWKNLNRSLNNLGAISSQQGNLSYVGKPN
                     AYQVNLNTQLLTTKTPVGDWQLQAKGNTQQLNIEHLVYQGQAGGVNAQGQLSWQQGLQ
                     LQGQFAVQDFRGDFWLPTWPTQLSGQSQIQLNINKNQKDITLSDSQLIGLVKNRAFNL
                     KTDLLAIDLQTLLAKKVPNFNNQQTQIIWGNNQLALEGGLQNNQWQLTVDNQLNDLNE
                     LLPSLQGKINGVLVLQGEQHKPAIISNLWAEKLAYNNIAVDELSISSTLPELGNVAGF
                     LQIAAHNLTAANRVIPDLT"
BASE COUNT         1481 a          733 c          957 g         1348 t
ORIGIN      
        1 ccacctaaca ttgagccaaa cagaatggct aaagaagtcc ccatttcgac taaggcatta
       61 ccaccgacta attcatgttt agttaatgct tggggcaaaa tggcgtattt aattgggcca
      121 aaagcggtgg agtgtgtacc cattaaaaat agggcggtga gtaataacca taattgctga
      181 aaatgaaagc caattgctgc caacagcata atgactattt cagccccttt aatgattttg
      241 attaatttcg ttttttcata tttatcagct agttggcctg ccgtcgctga aaataaaaag
      301 aatggtaaaa taaataagcc tgcacataaa tttgctaaag tgcttgcagg taaagtggtc
      361 atactggcgg cattaaaagc caataaaata attaaacttt gtttaaaaac attatcgtta
      421 aatgcaccta aaaattgtgt gctaaaaaaa ggtaaaaagc ggcgagtttt taaaagatca
      481 aattgattat gtgacatagt gtctatccgt agttatttta gttttagact aacattttga
      541 cgacgttttg taactatagc gatgataggc aagctatagt tgtgtaattc tcggtatgat
      601 taggtgagtt ttttaggatt gtgtgtagat gaagagacat tggtcacctg tttttgtttt
      661 atccttattc gtcacggtgg caaatgctgc cgataatcgt ttttcagagt tagaaaatta
      721 tgtctcacaa ttcaccaaac ctgatgaaaa aattaagtta agcatagaag gggtgtctgg
      781 tgaaattaag accaatgttg aagcttatat tggtgaatta acacaggaag atttgcaaca
      841 atggcgtgaa actcaagcac gcattcgcaa atcaagtcga gaagccttag agtcagtcgg
      901 ttattatcaa gctgatatta aaattgtcaa acaagataaa gcggtattac tcactattca
      961 gcctaatcag gctgtgattg tcagtaaaat caatctacag tatcagggtg aggcgagtaa
     1021 cgatattgcc tttactgcat taagagaaac ctttccctta aaagaagggc aagtcttgca
     1081 tcatggcaaa tatgaaaatg ccaaagcctt attacaaaat atggctttag aacgtggtta
     1141 ttttgaggct aaatggttaa cacatgaagt gattgttgag cagccaagcc aacaggccac
     1201 cattaattta gtgtatgaca gcggtaaacg ctacaaattt ggtcaaactt tttttagtgg
     1261 tgctaatcaa acacaattac ccgttaactt ggatttgttg gtaaagtttg taccttttaa
     1321 agaaggagac gattatgaag cggataaagt tattaaatta aataaaagtt tgctggatag
     1381 tcgttatttt aatcaagtta aagtgcgtgc tgaaagcgaa ttagcagaag aattttttgt
     1441 gcctgttaat gtcagtttag ctgccgaaaa acctaatcaa ttagatattg gtgctggcta
     1501 tgccactgat attggcgcaa gactaagtag tgcatggcgt aggccgttat taaatcaacg
     1561 gggtcatagt attgaagtag tcagtgagtt atcacaagtc aggcaatcat taaccatgcg
     1621 ttacggtatt ccgtggacac atccaattaa tgatacctta caaattagca caggttttaa
     1681 acgtgaagat attgacgata gtgctacaac taacaacagt gtgattggta tagagcggca
     1741 aaaaaagcgt gatagtggtt ggcaaaccaa tgagtcaatt cgatggagtc gagaatctta
     1801 tcatcaagat aatggcgaaa aaggacaaag tgatttgttg ttacccagtt atagtattag
     1861 tcgtgtgcgt actaaaggta gtaaaactga tcctgagcgt ggagatagac aatattatca
     1921 agtagaattt gcctcacccg atttattgtc agatgctgat ttattgtctt tacgagtggg
     1981 ttggcgttgg ctaaatacct ttgccaatcg tcatcaactg gcattgagag cagacttagg
     2041 ggctattttt agctctaatt ttgacagtgt gccattgaat atgcgttttt atgcaggtgg
     2101 cgaccagagt gtccgtggtt acgattataa aagtctttca ccgcgagata gtgcggggaa
     2161 agtgattggg gcgagtaact taatgtcgct cagtgctgaa tataccttta aattgacttc
     2221 aaaatggcgt ttagccagtt ttattgatag cggtaatgcc tttgattcgg ccagtgaacc
     2281 tttaaaaacg ggtgtaggct tgggcatacg ttggatatct cctgttggtc ctgtacgttt
     2341 agatgtggct ttgccattgg ataatacagc cgataataca ccacggattc atttttttat
     2401 ggggccagcc ttgtaatgct caaaaaaacg agtcgttttt tatggcggag tagttgtcat
     2461 ctgttggtgg cggtatggtt attgctattt ttagtatggc ttgttttggt gtcattggca
     2521 cattcgacca gtggttcacg ttggttgtta gaaaaaatcg ctcaacaaca aaagcttata
     2581 aagtatcagg ttgtatcggg taatttaatt gacggtataa cattaaaaaa ctttagattt
     2641 acgggtaaaa ctttttattt agatgctcaa gaattaacct tagatatttt atggataggc
     2701 ttgttaaata aagagttatt tgttgaaaaa ttacatggta acggtgtgca attagtatta
     2761 catggccaac ctaatccgca tcgtactcag ttaaaacatc tcaaaatacc gttgcgtata
     2821 gtcttaagtg atggtttgtt gctaaatgct aaaattaata agcgaggcgt gattacccct
     2881 attgatcgtt tagctttgca gcaagcccaa tggcaaggtg aagaccttaa gtttgctgaa
     2941 ttaaatgtaa tccatccgca attttcaact ttattacaag gacaattggc gtttaaagcc
     3001 gattatcctt tacaagcaat aggtattgtt caggccaagt tttggcaaaa acaaagtgta
     3061 aaacctatta aaatcaaagt aataggcgat ttgggtaatt tgggtgttaa tctagttgct
     3121 aaacctgtcg ccttaagcgc aacactggat gttttagagc caagtttgga ttatgtggct
     3181 aatgtgcggt gggggtattg gcaatttccg tggttaagcg cgcaaaattt tgtcagtcgt
     3241 tatgggcaag taaacattcg aggtaacaaa aagcatttag aactgagtgt aaatactgat
     3301 ttacaaagta atttgttgcc aagaggagag tatcgcgcct ttgccgaaac cgattggcag
     3361 catcttaaat tattaccttt agaagcaaga acgatactgg gtggaaaatt ggctttaaat
     3421 ggccaaataa gttggcaaaa tggcattcaa tggcaattaa ataccgattg gtcaaatgtc
     3481 gatttagcta aaaaatggcc agttattaac agttatttac ctaaactaac aggtaaatta
     3541 caaacacaag gtttggcttc ggcacaacaa tcaaaactta atattgatgc acaatgggca
     3601 aatggcgaac aatggcaaat aaaacagcaa aataaagact ggttttggca ttggcaacaa
     3661 cagcagcagc tacaagcttc atggaaaaac cttaaccgaa gtttaaataa tcttggcgca
     3721 atcagtagtc aacaaggcaa tttaagctat gtaggcaagc ctaatgctta ccaagtcaat
     3781 ttaaacacgc aattattaac cactaaaaca cctgtgggtg attggcaatt acaagctaaa
     3841 ggtaatacgc aacaattaaa cattgagcat ttggtctatc aaggtcaagc tgggggagta
     3901 aatgcccaag gacaactgtc atggcagcaa ggtttacaat tacaaggcca atttgctgta
     3961 caagattttc gtggtgattt ttggctaccc acatggccaa ctcaactctc aggtcaaagt
     4021 caaatacaac taaatattaa taaaaatcaa aaagatatta cattaagtga cagccaatta
     4081 attggattag ttaaaaatag agcatttaac ttaaaaacgg atttattagc cattgattta
     4141 caaacactgt tagctaaaaa agtgcctaat tttaataatc agcaaacgca aattatttgg
     4201 ggtaacaatc aattagccct agaaggtgga ttacagaata atcaatggca actaactgtt
     4261 gataatcaac tgaatgactt aaacgaatta ttgccgagtt tacaaggcaa aataaatggt
     4321 gtgttagtct tacagggtga gcaacataaa ccagccatta tcagtaattt atgggccgaa
     4381 aaattagcct acaataatat cgcggttgat gaactcagta ttagtagcac attaccagag
     4441 ttaggtaatg tcgcaggatt tttgcaaatt gctgcacata atttgactgc ggctaatcga
     4501 gtcattcccg atttgacca
//
LOCUS       DAOQMB010000171         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9213_length_4507_cov_17.052561, whole genome shotgun sequence.
ACCESSION   DAOQMB010000171 DAOQMB010000000
VERSION     DAOQMB010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9213_length_4507_cov_17.052561"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLY05_06740"
     CDS             complement(<1..311)
                     /locus_tag="PLY05_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03037.1"
                     /translation="MSQRPVGQFARHFFDNFIAALLMLLGLKRAFTHLHPTPVQFLSF
                     LLGSLLTSFSFDLISQGLDGELQPVGFAAYIIPPFLLLIVGLFLSQRYGLWRLTLAPV
                     I"
     gene            complement(380..1063)
                     /gene="bioD"
                     /locus_tag="PLY05_06745"
     CDS             complement(380..1063)
                     /gene="bioD"
                     /locus_tag="PLY05_06745"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HNC03038.1"
                     /translation="MPTYFIAGTDTHIGKTTIACALLAKAQQHGLSTLGIKPIASGCE
                     MTDDGLRNSDALALQAQSSIKVPYQEINPVTLAEPLSPHIAAQRANKRLTINQLVGYT
                     RATLSHRPQFAVVEGAGGWRVPISDRELLSALPKELKLPVILVVGIRLGCLNHAILTA
                     EAIAKDGLRLAGWVANCIDPEMAALEENIQTLKRILPAPCLGVVPFQTTAHIHVAEFV
                     NLPDETINK"
     gene            complement(1076..2005)
                     /locus_tag="PLY05_06750"
     CDS             complement(1076..2005)
                     /locus_tag="PLY05_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03039.1"
                     /translation="MRKIVTASLLALSLALTACASIETKLSDWAISMERSQADLELKE
                     IVVGEFTTPYLEGGQGETVLLIHGFQSNKDIWIRMAKQLTKNYHVIAIDLPAHGDSNI
                     LMDKSYTVPEQAKRVIAIMDKLQLTQPVHIMGHSMGGAISFHVAHLAPSYVKSLALMN
                     SAGVISPQPSELYLRNQKGENPLIVRNEEDYKKMLAFTMSEPPYIPSPVIATLTRISI
                     SREKLGEKIYQELNLKYSLNPEEILPQIKVPTLVLWGDQDKVINVSSTEVFKRLMPQA
                     QVMIFKGVGHAPQIERTKEAAQTYKAFLANIKS"
     gene            complement(2013..2801)
                     /gene="bioC"
                     /locus_tag="PLY05_06755"
     CDS             complement(2013..2801)
                     /gene="bioC"
                     /locus_tag="PLY05_06755"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HNC03040.1"
                     /translation="MTMLFNERIAQRFGSAAAHYDEQALAQRQSAQQLMVGQILQGRV
                     LDIGCGTGWLTRDIAEHHPIDVIVALDIALPMLKAEQLQHPSIIPIQADAAFLPFQNN
                     SFDAVVSNFALQWLASPQNFAQELARVLAKDGQFCLAIPVNGTLRELNQAWAQLDIRR
                     HTNQFFATQTWLDAFRYVGLTIQSQQQSAFYQYYDSTKSLLKSLKAIGANELQQPRQQ
                     GLWGRGQLLALEHAMEHYRQSQGIPLHYEVLMVYGQKVNLQHNI"
     gene            complement(2785..3561)
                     /locus_tag="PLY05_06760"
     CDS             complement(2785..3561)
                     /locus_tag="PLY05_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03041.1"
                     /translation="MSKLKVRHYSQKPLEIVLIHGWGLHSGVWQKLIEYLQTFANITV
                     IDRAGYGHSQIMSHEDELNEILASCPPKAVYIGWSLGVSVILQLATQHPQRVQAIVAL
                     AADPCFVARKDWPHAMPIETFQQFQQDTEENAYTALLRFMGLQTQGSNTQRQELRFLQ
                     QLLAQHPIPHHLQLLQGLADLAQDYRPLVEKIRCPMLWVYGTKDKLVNIDVDALLALN
                     SQITFGSFADAAHVLLLSHPEPLSDMIIRFLRGNGYDDAV"
     gene            complement(3554..>4507)
                     /gene="bioF"
                     /locus_tag="PLY05_06765"
     CDS             complement(3554..>4507)
                     /gene="bioF"
                     /locus_tag="PLY05_06765"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR00858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HNC03042.1"
                     /translation="SGAAHLVCGHHRQHQALEEELAAFCQRPAALLFSTGYMANLGVI
                     NALVGRGDAVFEDRLNHASLLDGGLSSGARFSRYQHNDVEDLQQKLQANDSLKKLVVT
                     DAVFSMDGDMALMPELVTVSQQQQAALMIDDAHGFGVLGNTGAGTSEHFGCTVDDVPI
                     YMATLGKAVGTFGAFVAGSQELIDYLRNFARPYIYTTALPPAIAAATRQSLRLIAEET
                     WRREQLQAHIQIFRQQVLAQGWSLMASDTAIQPLLVGDEQRALQLSQALADKGFWVTA
                     IRPPTVPVGESRLRVTLTAAHKTADVQALINALAELRTNDV"
BASE COUNT         1324 a         1024 c          884 g         1275 t
ORIGIN      
        1 aaaatgacag gagctaaagt taaacgccac aagccataac gctgtgacag aaataagcca
       61 acaatcaata acaaaaatgg tgggataata taggcagcaa aaccaacagg ttgtagttct
      121 ccgtctaaac cttgactaat taaatcaaag ctaaaactgg ttaataagct tcctaataaa
      181 aaacttaaaa actgcacagg tgtgggatgt aaatgggtaa atgcccgctt taagcctaat
      241 agcatcaata aagcagcgat aaaattatcg aaaaaatggc gggcaaattg gccaactggc
      301 cgttgagaca tgattgttat cctaataaat tagcgcgcga atcatagcat aaataaacct
      361 cagtaaatgc aagtggctat cacttattta ttgtctcatc aggcaaattt acaaactcag
      421 cgacatgaat atgcgctgtg gtttgaaaag gcacaacccc caaacagggt gcaggcaaaa
      481 tacgttttaa ggtctgaatg ttttcttcta aagcggccat ctctgggtcg atacaattag
      541 ctacccaccc tgccaaacgt aaaccatctt tcgcaatggc ctctgcggtt aaaatggcat
      601 gattcagaca acccaaacgt attcccacca ctaaaatcac aggcaatttt aactctttag
      661 gcaatgccga taatagctct cggtcactga ttggtacacg ccaaccacct gccccttcaa
      721 ccaccgcaaa ctgtggacga tgacttaacg tggcgcgggt ataaccgact aattgattga
      781 tcgttaaacg tttattggcg cgttgtgcgg caatgtgtgg cgacaaaggt tcagctaggg
      841 tgacaggatt tatttcttga taaggcactt ttatgctgct ttgcgcttgc aaggctaaag
      901 cgtcactatt acgcaaaccg tcatccgtca tctcacaacc tgaagcaatc ggttttatcc
      961 ctaaagtgct taaaccgtgt tgttgagcct tagctaacaa cgcgcaggcg atggtggttt
     1021 tgccaatatg cgtatctgtg cctgcaataa aataagtggg catttatcaa atcctttaag
     1081 attttatgtt ggctaaaaat gccttatagg tttgtgcggc ttctttagtg cgttcaattt
     1141 gtggcgcatg accgacgccc ttaaaaatca tgacctgtgc ctgaggcatc aatcttttaa
     1201 aaacttctgt gctagacaca ttaataactt tgtcttgatc gccccataat accaacgtcg
     1261 ggactttgat ttgcggtagt atttcttcgg gatttaacga atatttgagg tttaactctt
     1321 gatagatttt ttcacccagt ttttcacgag agatagaaat acgcgttaaa gtcgcgataa
     1381 caggtgaagg tatataaggt ggctcggaca tggtaaaagc taacattttt ttataatctt
     1441 cttcattacg cacaattaac ggattttcgc ctttttgatt acgcaaatat aactcactcg
     1501 gttgcggcga aatcactcct gccgaattca ttaatgccaa acttttaaca tagcttggcg
     1561 cgaggtgtgc gacatgaaat gaaatcgcgc cacccatcga atgccccata atatgtactg
     1621 gctgcgttaa ttgtagtttg tccatgatgg caattacccg tttagcttgt tcaggaacgg
     1681 tatagctttt atccatcaaa atattgctat cgccatgcgc gggtaagtca atggcaatca
     1741 cgtgatagtt tttggtcagt tgtttggcca tcctaatcca aatatcttta ttcgactgaa
     1801 aaccatgaat taataatacg gtttctccct gaccgccttc taaatagggc gtagtaaact
     1861 ccccaacgac gatttctttg agctcaaggt cggcttgtga ccgctccata ctaatcgccc
     1921 aatctgataa ttttgtctca atactggcac aagctgttaa tgccaagctc aaggctaata
     1981 aacttgctgt aactattttt ctcatggttt actcagatat tatgttgtaa attcactttt
     2041 tgaccgtaca ccatcaagac ttcatagtgt aaaggaatgc cttgtgactg ccgatagtgt
     2101 tccatcgcgt gctctaaggc taataattgc cctcgccccc ataaaccttg ttgacgtggt
     2161 tgttgcaact cgtttgcacc tatcgctttc agactcttta ataatgattt tgttgagtca
     2221 tagtattgat aaaacgcgct ttgttgctga ctttgtattg ttaaacctac gtatcggaat
     2281 gcgtctaacc atgtttgtgt agcaaaaaat tgattagtat gacgtctaat atcaagctgc
     2341 gcccatgcct gatttaattc tcttaatgtt ccatttacag gaatcgctaa acaaaactga
     2401 ccatcttttg ctaatacacg cgctaattct tgagcaaaat tttgcggcga tgccagccat
     2461 tgcaaggcaa aattactcac tactgcatca aagctattgt tttggaacgg aagaaaagcg
     2521 gcatcagctt gaatgggaat aattgaggga tgttgtaact gctcggcttt taacatcggc
     2581 aaagcaatat ctaaagcaac gatgacatca atcggatgat gttcagcaat atctcgcgta
     2641 agccaacctg tgccacaacc aatatctaag acccgacctt gtaaaatttg gccaaccatc
     2701 agctgttgtg ccgattgacg ttgtgccaaa gcttgttcat cgtaatgagc tgctgccgag
     2761 ccaaaacgct gagcaatccg ctcattaaac agcatcgtca tacccattac cacgcaaaaa
     2821 tcgtataatc atgtcgctta agggttcagg gtgcgacaac aataaaacat gtgctgcatc
     2881 cgcaaaagaa ccaaaagtga tttgagaatt aagcgccaat aacgcatcaa catctatatt
     2941 taccaattta tcttttgtac catataccca taacatcgga caacgaatct tttctactaa
     3001 agggcgataa tcttgcgcta aatcggctaa accctgtaaa agctgtagat gatgaggtat
     3061 ggggtgttgt gctaaaagtt gttgtaaaaa acgcaactct tgacgttgtg tgtttgagcc
     3121 ttgtgtttgt aaccccataa aacgcaataa agcggtataa gcattttctt cagtatcttg
     3181 ttgaaattgc tgaaaagttt ctataggcat ggcatgaggc caatctttac gcgcgacaaa
     3241 acaagggtcg gcagctaatg cgacaatcgc ttgtacacgt tgcggatgct gagtggccaa
     3301 ttgcaaaatc acactcacac ccaatgacca accgatgtac actgcttttg gtggacaact
     3361 tgctaaaatc tcatttaatt catcttcatg gctcattatc tgactatggc cataacctgc
     3421 tctatcaatc accgtaatgt tggcaaacgt ttgtaaatat tcaatcaatt tttgccatac
     3481 accactatgc aagccccaac catgaatgag tacaatctct aatggttttt gtgaatagtg
     3541 gcgtactttt agtttagaca tcgtttgtcc tcaactccgc taaggcattg atgagcgctt
     3601 gcacatccgc cgttttatga gcagcggtta aggtaactct taagcgcgac tcgccaacag
     3661 gcacagtggg tggacgaatt gcagtcaccc aaaaaccttt atcagccaaa gcctgactta
     3721 actgtaaggc acgttgctca tcacccacca ataaaggttg aatcgcggta tcgctggcca
     3781 ttaatgacca accttgtgct aaaacttgtt gtctaaaaat ttgaatatgc gcttgtaatt
     3841 gttcgcgtcg ccatgtttct tcagcaatta aacgcaaact ttgtcgtgtg gctgccgcaa
     3901 tagcaggcgg taaggcagtg gtataaatat agggtcgggc aaaattgcgt aaataatcaa
     3961 tcaactcttg gctacccgcg acaaatgcac caaacgtccc caccgcttta cccaaggtgg
     4021 ccatataaat cggcacatca tccaccgtac agccaaaatg ttcgcttgta cccgcacctg
     4081 tgttacccaa cacgccaaaa ccgtgggcat catcaatcat taatgccgct tgctgttgtt
     4141 ggctgaccgt taccaattca ggcattaatg ccatatcgcc atccatgcta aacacagcat
     4201 cagtcaccac taattttttc aggctgtcat tcgcttgtag tttttgctgc aaatcttcaa
     4261 catcattatg ttgataacga ctaaaacgcg caccgctgct taaaccacca tctaataaag
     4321 aagcatggtt caaacggtct tcaaatactg catctccacg cccgactagc gcatttatca
     4381 cccccaaatt agccatatac cctgttgaaa ataataaagc cgctggacgt tgacaaaaag
     4441 cggctaattc ttcttcaagg gcttgatgtt ggcgatgatg accacaaacc aaatgtgctg
     4501 caccact
//
LOCUS       DAOQMB010000172         4491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9273_length_4491_cov_16.847610, whole genome shotgun sequence.
ACCESSION   DAOQMB010000172 DAOQMB010000000
VERSION     DAOQMB010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_4491_cov_16.847610"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLY05_06770"
     CDS             complement(<1..491)
                     /locus_tag="PLY05_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03043.1"
                     /translation="MIRVLVVDDHEVIRIGLKGTLKDIKDILVVGEAKTGEEAVKIIP
                     LLHPNVVLMDVQMPGMDGIEATRKIVKHYPSSKILALSAHDEEPFLSNILAAGALGYV
                     TKGASLDEMIEAIHQVAAGQRYFGKDIASRVASNLLDKKTSPFALLSPRERQMAEMVV
                     NGQT"
     gene            1148..2452
                     /gene="pbpG"
                     /locus_tag="PLY05_06775"
     CDS             1148..2452
                     /gene="pbpG"
                     /locus_tag="PLY05_06775"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HNC03044.1"
                     /translation="MMKFKLALLPSLVLGLMLAVAPIQQTNAKEQTPAKVKVAKNDKK
                     ASADKAAKNKDKKTDSKVSAKKADKDKKLAKNATKNKKTTDLADKKSAKVKAKEARLA
                     AKEKNAKELRLAEKKQAERQKERLAERKERTRERLAQVSRTGRVAKVAKYAARPVVVA
                     AATASTAAIAANQINKPVIEQSNDIAQSLNLESKAALVMNAVTGEVLYAKNTNQAVPI
                     ASITKLMTAMVVLDAKLPLDEAITISEEDIDRMRNTSSRLSVGTVLSRAELLLLALMS
                     SENRAAHALARTYPGGVNAAVAAMNRKAAALGMTQTRFEDGTGLSSNNVASPKDLVAL
                     VKTSNTYPLIRRFSTTGGHAVAIKDSVQQFNNTNALVKNPDWEIGVSKTGFINEAGKC
                     LVMQAKIKSTPVVMVLMDSWGKYTRIGDAVRVKKWLESSSTN"
     gene            2527..3069
                     /locus_tag="PLY05_06780"
     CDS             2527..3069
                     /locus_tag="PLY05_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HNC03045.1"
                     /translation="MHEQYALSEETLTNLEDYFDSEDNEYGLNFCGTHGFLCAITVGP
                     NTDKTIWLDALFEGQVPQEWIADLEAWQKSIHALLYHEQTLRLPCDLVVSLDDDNDLT
                     DWCVGFMEGMFCNEEAWYAKNEDLVIELTLPIVSISGLIEDPELNKIRKNRKLLQQFA
                     REIPEVLTELYLLFNAPQTP"
     assembly_gap    3070..3169
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3170..4038)
                     /locus_tag="PLY05_06785"
     CDS             complement(<3170..4038)
                     /locus_tag="PLY05_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HNC03046.1"
                     /translation="MESIVVAALYQFKAVADPANLQQSLKDLCKTQEILGTLIVANEG
                     INGTVSGSRQAIDVLHQFLLAQGFDRMEYKESFAPEHTFRKLKIKLKKEIVTLGVHVD
                     PRDHVGTYLEPEQWHEFIQQDDVIIIDTRNDYEYVTGTFEGAIDPQTKSFGEFPQYVQ
                     EHLKDAKDKKIAMFCTGGIRCEKSTSYLLQEGFKEVYHLKGGILNYLAHIPDEKSLWK
                     GECFVFDRRVGVGHGVNLGDTAMCFGCGHPLFAGDTDSPKYEEGVSCPHCYDHTTEEQ
                     KARFRMRQSQHVL"
     gene            4066..4491
                     /locus_tag="PLY05_06790"
     CDS             4066..4491
                     /locus_tag="PLY05_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004640373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HNC03047.1"
                     /translation="MPNSRPKTPCVGLCSTVFGDTVCRGCMRFVHEVIDWNKYNPEQK
                     QIIWQRLDEHLRVILPQFVKIYDPRKIVARLQQQRIIYDEKNLWRGIYSLLRLHDKGF
                     IVLADLGFQVKPDYQWILVQQQLYQLASAYYQKDFARHL"
BASE COUNT         1423 a          836 c          768 g         1364 t
ORIGIN      
        1 gtttggccat ttacgaccat ttctgccatt tgcctttcac gcggagataa caaagcaaaa
       61 ggagatgttt ttttatctaa taagttactt gctactcgcg aggcaatatc tttaccaaaa
      121 tatcgctgac ctgccgctac ttgatgtatg gcttcaatca tttcatctaa ggatgctcct
      181 tttgtcacat aacccaaagc gcctgccgcc aaaatattag ataaaaaagg ttcttcatca
      241 tgcgcagata atgccaatat tttactgctt ggataatgtt tgacaatttt gcgcgtcgcc
      301 tcaataccat ccatacctgg catttgtaca tccatcaaaa caacattagg atgcaataaa
      361 ggaattattt ttacagcttc ctcacctgtt ttggcttcgc ccacaactaa tatatcttta
      421 atatctttta aggttccctt taaccctata cggataacct cgtgatcatc aaccactaag
      481 actctaatca aaatatcacc ccatcggttt ttagatatca tttattataa taagattaat
      541 gctttacgac ttatatcaca ataaaaaatg cagataaaat aagtaaaata atagaatctt
      601 gaagaatatg aaatattagt aatgctttag acagatatag caaaaactct cagccaattt
      661 cttaacaaaa agtggcttat atcacatttt ttgtctataa tttcgacagt ttttgtctta
      721 caataaacac aatatttttt tataaattgg tataaaacat ttagttcaga agtcatgcac
      781 gaaacattga atattaccta aattggtcta tgcttatatt aatagctaat taatttgtta
      841 caaaatcttg tgtttagctg tcacgctctt tgcatattac cttaagtagt tcatgcttaa
      901 agtattgtga aatagcataa atcggtttta gggcaaattt atgtattaat ctcatcaaac
      961 aataggatgt gtctgatgtg aaacgctaag acgcagctta tcaatcatga ctgccgctca
     1021 atagcaataa actttcagga catttaaaaa ataggatttt aaatcctatt ttttaattaa
     1081 attaaatttt tcactactct ctagtgacga gtatgttttt tgtttttata aacatgggtc
     1141 atatattatg atgaagttta aattggcgtt attaccttct ttagtgttag gtttgatgtt
     1201 ggccgttgcg cctattcaac aaactaatgc taaagaacaa actccagcta aggttaaagt
     1261 ggctaagaac gataaaaaag cctcagccga caaagctgcc aaaaataaag ataaaaagac
     1321 cgatagcaaa gttagtgcta aaaaagctga taaagacaaa aagctcgcaa aaaacgccac
     1381 taaaaacaaa aaaacgactg atttagcaga taagaaatca gcaaaagtca aagctaaaga
     1441 agctcgttta gctgcgaaag aaaaaaatgc taaagagctt cgtttagccg aaaaaaaaca
     1501 agcagaacgc caaaaagaac gtttagcaga acgtaaagaa cgtactcgtg agcgtttagc
     1561 tcaagtttct cgtacaggtc gcgtcgccaa agttgccaaa tatgcagctc gtcctgttgt
     1621 ggttgctgcg gcaactgcta gcactgccgc aattgcagct aatcaaatca ataaacctgt
     1681 gattgaacaa agcaatgata ttgcccaaag cctaaattta gagtctaaag cagcattagt
     1741 gatgaatgct gttacaggtg aagtgcttta tgctaaaaac actaatcaag cagtgccaat
     1801 tgcgtctatt actaaattaa tgactgcgat ggtggttttg gatgccaaat tgcctttaga
     1861 tgaagcaatt accattagcg aagaagacat tgatagaatg cgcaatacta gttctcgctt
     1921 aagtgtgggt acagtgttat ctcgtgcaga attattatta ttggccttga tgtcttctga
     1981 aaatcgcgct gctcatgctt tagcacgtac ttatcctggt ggtgttaatg cggctgttgc
     2041 tgcgatgaac cgtaaagcag cagctttagg catgacacaa acacgctttg aagatggcac
     2101 tggtttaagt agcaacaatg ttgcttctcc taaagattta gtggcacttg ttaaaacatc
     2161 taatacttac ccattaattc gtcgtttttc caccacaggt ggtcatgccg ttgctatcaa
     2221 agactcagta caacaattta ataacaccaa tgcattagtt aaaaatcctg attgggagat
     2281 tggcgtttct aaaacaggct ttattaatga agcaggcaaa tgtttggtga tgcaagccaa
     2341 aatcaaatcg acacctgttg ttatggtgtt gatggactct tggggcaaat acacgcgtat
     2401 cggtgatgca gttcgtgtga aaaaatggtt ggaatcaagt tctaccaact aaaatactcg
     2461 ctattctgtt aaaaagcctc taatcttaga ccataaaatt agaggctttt ttattggaat
     2521 attcttatgc atgaacaata cgctctttct gaagaaaccc taacaaacct agaagactat
     2581 tttgatagtg aagacaatga atatggctta aacttttgtg gcacacatgg ttttttatgt
     2641 gccattactg tagggccaaa taccgataaa accatttggc tagatgcctt atttgaaggt
     2701 caagttcccc aagaatggat tgccgattta gaggcatggc aaaaaagtat tcatgcttta
     2761 ctttatcatg aacaaacttt acgtttaccg tgtgacttag ttgttagctt ggatgatgac
     2821 aacgacttaa ccgattggtg tgttggcttt atggaaggta tgttttgcaa cgaagaagca
     2881 tggtatgcca aaaatgaaga cttggtgatt gagctaacat taccgattgt tagcattagt
     2941 ggtctcattg aagaccctga attaaataaa atccgcaaaa atcgcaagtt attacaacag
     3001 tttgcccgcg aaattcctga agtactgaca gagttatatt tattatttaa tgcgccacaa
     3061 acgccttaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttaatacatg
     3181 ctgactttgg cgcattctaa aacgtgcctt ttgttcttct gttgtatggt cataacaatg
     3241 agggcaactc acgccttctt catattttgg gctatcggta tcgcctgcaa acaacggatg
     3301 accacaacca aaacacatcg ccgtatcacc taaattgaca ccatgcccca cacctacacg
     3361 acgatcaaag acaaaacatt cgcctttcca caaacttttt tcatctggaa tatgggccaa
     3421 ataatttaaa ataccacctt taaggtgata aacctcttta aaaccttctt gcaataaata
     3481 acttgtggat ttctcacaac gaataccacc cgtacaaaac atcgcaattt ttttgtcttt
     3541 cgcatctttt aaatgctctt gaacatattg gggaaactcg ccaaagcttt tcgtttgtgg
     3601 atcaattgcc ccttcaaacg tgcctgtgac atattcataa tcattacgcg tatcaataat
     3661 aattacatcg tcttgttgaa taaattcgtg ccattgctca ggctctaaat aagtaccaac
     3721 atgatctcgt ggatcaacat gtacgcctaa ggtgacaatc tcttttttaa gtttaatttt
     3781 taactttcgg aaagtatgtt caggtgcaaa agattctttg tattccatac ggtcaaaacc
     3841 ttgtgctaat aaaaattgat gtaagacatc aattgcctga cgactaccag agaccgtacc
     3901 gttaatacct tcattagcca caatcagtgt tcctaaaatt tcttgtgttt tacacaggtc
     3961 ttttagagac tgttgtagat ttgcagggtc agcaactgct ttaaactgat ataatgcggc
     4021 aacaacgata gattccataa cttaaccttt taaacttaaa aactcatgcc caattcgcgt
     4081 cccaaaaccc cttgtgttgg tctgtgctct actgtatttg gtgatacggt ttgtcgtggc
     4141 tgtatgcgtt ttgtccacga agtgattgat tggaacaaat ataatccaga gcaaaaacaa
     4201 attatttggc agcgattaga tgaacatttg cgagtgattt taccacaatt tgtcaaaatt
     4261 tatgacccac gcaaaattgt cgctcgttta caacaacaac ggattattta tgacgaaaaa
     4321 aatctatggc gtggtattta tagtttatta cgtttacacg ataaaggttt tattgtttta
     4381 gcagacttag gctttcaggt taaacccgat tatcaatgga ttttagtcca acaacaactc
     4441 tatcaattag ctagtgctta ttaccaaaaa gattttgcca gacatttata a
//
LOCUS       DAOQMB010000173         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9276_length_4490_cov_8.003157, whole genome shotgun sequence.
ACCESSION   DAOQMB010000173 DAOQMB010000000
VERSION     DAOQMB010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9276_length_4490_cov_8.003157"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLY05_06795"
     CDS             complement(<1..396)
                     /locus_tag="PLY05_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03048.1"
                     /translation="MENLETIAEHSLGIVEHISKRAMQDLGFTDYGAEVPHSNITDNI
                     INIIQDSIDSVRRDNGVLALRPTFMRVDLEDIDENYCFSLYVTPVAPPTGLDGWRAKQ
                     LNFVSYRTDAGRVAELPCGSDFELQGKEYY"
     gene            complement(471..3191)
                     /locus_tag="PLY05_06800"
     CDS             complement(471..3191)
                     /locus_tag="PLY05_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNC03049.1"
                     /translation="MAIRRFSSRTERLDTEFLAQSLQGAAKYFRIAGYFRSSIFELVG
                     EQIAQIPEVKIICNSELDIADFQVATGRNTALKERWNAIDIEAEALLKKERYQILDQL
                     LQSGNVQIRVVPKERLFLHGKAGSIHYYDGTRKSFIGSVNESKSAFAHNYELLWQDDD
                     EESADWVEKEFWALWSEGIPLPETILAEINRVANRREITVDVLKPKDVPAAAMAEAPI
                     YRGGEQLQPWQRSFVAMFLEHREIYGKARLLLADEVGVGKTLSMATSALVSALLQDGS
                     VLILAPSTLTVQWQVEMMDKLGVPTAVWSSQRKVWLGVEGQMLSPRDDASSIKKCPYK
                     IAIVSTGLIMHQREHGQFIKEAGMLLKNKFGTVILDESHKARVRGGLGESALEPNNLL
                     AFMRQIGHRTRHLILGTATPIQTNVRELWDLLGILNSGAEFVLGDALSPWLDHEQATA
                     LITGKTVVQSEQEVWQWLSNPLPPANEHHLIQQVRDSLGIDPKSFVCSYRFDDLDYMI
                     KNMWLSECLAPEFFEENNPVLRHTVLRKRRQLEEDGLLEKVGVNTHPNINNLAQYQHR
                     FVGLGIPTNIPFQVAYEKAEEFSSLLQSRTKSGGFMKSLMLQRICSSFASGLKTAQKM
                     LEHTLPNEESEGLDNTDDHILSAMTSAEVACLKEIVTQLSRPEAIDSKLETVKWFLTD
                     FRTDGKTWLEHGCIIFSQYYDTAQWIAKELAKAYVGEVVAVYAGVGKSGLFKGEQFNH
                     VEREVIKSAVKKREIRLVVATDAACEGLNLQTLGTLINVDLPWNPSRLEQRLGRIKRF
                     GQSRKFVDMLNLVYSETQDEKVYNVLSERLRDTYDIFGSIPDIIDDDWIADEEKLKDR
                     MDEYMHERKKAQDAFSVRYRDTIDPQAHLWERCANVLSRRDIVSKLSEPW"
     gene            complement(3195..>4490)
                     /locus_tag="PLY05_06805"
     CDS             complement(3195..>4490)
                     /locus_tag="PLY05_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004965098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HNC03050.1"
                     /translation="GYIQGLFDLNVKEFPIAGNAEINSHPAQNLETENDIYITDPPYG
                     DAVKYEEITEFFIAWLRKNPPAEFAHWTWDSRRSLAIKGEDEGFRQGMVAAYRKMTQK
                     MPDNGIQVLMFTHQSGSIWADMASIIWASGLQVTAAWYVVTETDSALRQGANVTGTIM
                     LVLRKRHKALETFRDDLGWEIEEAVKEQVASLIGLDNSVREQGSEGLYNDADLQMAGY
                     AAALKVLTAYSRIDGKDMVVESEAPRQKGKKTFVDELIDFAVQTAVQFLVPVGFEKAE
                     WQKLQAIERFYLKMAEMEYQGAKTLDNYQNFAMALKVHHFEDVMSDSSKANAARLKLS
                     TEFKSALLSGDAEFANTPLRALLYAMFELSKEKEIEDVLYTLTENCPNYLQNKQLLAK
                     MADYLAEKRGVLKGTKTFTPEHEASYARILAEAIRTHRL"
BASE COUNT         1247 a         1021 c          812 g         1410 t
ORIGIN      
        1 ataatactct ttaccttgca gctcaaagtc tgagccacaa ggcaactcgg caactctacc
       61 tgcatcagtt ctatagctaa caaaatttag ttgtttagcc ctccacccat ctaatccagt
      121 aggaggtgcg acaggagtca catacaaact aaaacaataa ttttcatcta tatcttctaa
      181 atcaactcgc ataaacgtgg gtcttaaggc taaaacacca ttatcgcgtc taactgaatc
      241 aatagagtct tgaattatat ttattatatt atctgtaata ttagagtgtg gaacttctgc
      301 accataatcg gtaaaaccaa ggtcttgcat agctcgtttt gaaatatgtt ctacaatgcc
      361 taaagagtgt tcagcaatcg tttctagatt ttccatgtat ccaccaatct attaatgttg
      421 atatatttta agttctatca ttgttgttat ctgtacaatt aacctcaaac ctaccaaggc
      481 tcgctcaatt tactgacaat atctctacgt gacaaaacat ttgcgcaacg ctcccataaa
      541 tgcgcttgag ggtcaatagt gtctctgtac cttacactaa aagcatcttg agcttttttt
      601 cgctcgtgca tgtattcatc catacggtct tttaattttt cttcatcagc aatccagtca
      661 tcatcaataa tatctggaat actgccaaaa atatcgtaag tatcacgtaa gcgttctgac
      721 aacacgttgt aaactttttc atcttgtgtt tcgctataaa caagattgag catatcaaca
      781 aatttacgac tttggccaaa tcgtttaata cggcctagtc gttgttctaa acgagaagga
      841 ttccaaggaa ggtcaacatt aattagcgta cccaaagttt gtaaattgag tccttcacaa
      901 gctgcatccg ttgcgactac cagtctaatc tccctctttt taaccgctga cttaatcact
      961 tctcgctcaa catggttaaa ttgctcacct ttaaacaagc cacttttacc tacgccagca
     1021 taaacggcaa cgacctcgcc tacataggct ttcgctaact ccttcgctat ccattgtgct
     1081 gtatcatagt actgactaaa aataatacag ccatgctcta accatgtttt accgtctgtt
     1141 ctaaaatccg ttaaaaacca cttaaccgtt tcgagtttgg agtcaatggc ttcaggacga
     1201 gaaagctgag tcacgatttc ttttaagcaa gcaacttcgg cagaagtcat tgccgacaag
     1261 atatggtcat cggtattatc taatccctca ctttcttcgt ttggtagcgt atgttctaac
     1321 attttttgtg cagtttttaa gcctgacgca aagctagagc aaatacgttg caacatgagt
     1381 gatttcataa aaccacccga tttagtgcga gattgaagta atgagctaaa ttcttcggct
     1441 ttttcgtagg ccacttggaa tggaatattt gttggaatgc ctaaaccaac aaaacgatgt
     1501 tgatattgtg ctaaattgtt aatgttggga tgtgtgttaa caccgacttt ctcaagtaat
     1561 ccatcttctt ctaattgccg tcttttacga agaacggtat ggcgcaaaac aggattattt
     1621 tcttcaaaaa actctggggc taaacattca gataaccaca tgtttttaat catgtaatca
     1681 aggtcatcaa aacgataact acacacaaat gatttagggt ctatccctaa tgaatcacga
     1741 acctgttgta tcaaatgatg ctcatttgct ggtggcaaag gattagataa ccattgccaa
     1801 acttcttgct cagattgcac aaccgtttta cctgttatca aggcagtcgc ttgttcatgg
     1861 tctaaccaag gcgaaagtgc atcacctaat acaaactcag caccactatt taatatccct
     1921 aataagtccc acaactctcg tacattcgtt tgaataggcg ttgccgtacc caaaattaaa
     1981 tggcgtgttc gatgccctat ttgcctcata aaagccaata agttatttgg ctctaatgca
     2041 ctttcaccaa gaccacctct gactcgtgct ttatgtgact catctaaaat aacagtacca
     2101 aatttatttt ttaaaagcat acctgcttct ttaataaatt gaccatgttc gcgctggtgc
     2161 ataataagcc ctgtcgagac aatcgcaatt ttatacggac atttttttat agaagaagca
     2221 tcgtcacgcg gcgatagcat ttgaccttca acacccagcc acacttttcg ttgtgatgac
     2281 caaacggcag taggaacacc taatttgtcc atcatttcaa cttgccattg aaccgtcagt
     2341 gtagaaggtg ccaaaatcag cactgaacca tcttgaagta aggcactcac tagcgcactg
     2401 gtcgccattg acaatgtttt acctacaccc acttcatctg ccaataacaa cctagcttta
     2461 ccatagattt ctctatgttc taaaaacatc gccacaaaag aacgctgcca aggctgcaac
     2521 tgttctccac ctctataaat aggtgcttcg gccattgcag ccgcaggaac atctttgggt
     2581 tttaaaacat ctaccgtgat ttctcgacgg ttcgctactc tgttaatttc ggctaaaatt
     2641 gtttcgggta atgggattcc ttcactccac aaagcccaaa actctttttc tacccaatcg
     2701 gcactttctt cgtcatcgtc ttgccataac aattcgtaat tatgggcaaa agcacttttt
     2761 gattcattca ctgagccaat aaacgattta cgcgtgccgt cataatagtg aatagagcct
     2821 gctttaccat gcaaaaaaag tcgttctttt ggcacaactc gaatttgcac attacccgat
     2881 tgcaataatt ggtcaagaat ttgataacgc tcttttttta ataaggcttc ggcttctata
     2941 tcaatggcat tccaacgctc ttttaaagcg gtattacgtc ccgttgcgac ctgaaaatcg
     3001 gcaatatcta attcagaatt acaaataatt ttaacttcgg gtatttgtgc aatctgttca
     3061 cccaccaatt caaaaataga gcttctaaaa taccctgcaa tgcgaaagta ttttgcagct
     3121 ccttgtagcg attgggctag aaattccgta tctaatcttt cagtgcgaga tgaaaaacgc
     3181 cgaatcgcca tgttttacaa cctatgagtt ctaattgctt ctgccaaaat ccgtgcataa
     3241 ctggcttcat gttctggcgt aaaggttttt gtgcctttaa ggactccacg tttttcggcc
     3301 aaataatcgg ccatttttgc cagtagttgc ttgttttgta aataatttgg gcaattttct
     3361 gtgagcgtat aaagcacgtc ttctatctct ttttcttttg aaagctcaaa catggcataa
     3421 agcaatgctc ttagtggtgt gtttgcaaac tctgcatcac ccgacaacag ggctgattta
     3481 aactctgttg atagttttaa gcgtgcggca ttggctttgg agctatcact catcacgtct
     3541 tcaaaatgat gtacttttaa agccatagca aagttttgat agttatcgag tgtttttgcg
     3601 ccttgatatt ccatctcggc cattttgaga taaaaccgtt cgatagcttg tagcttttgc
     3661 cattcggctt tttcaaagcc aacaggcacc aaaaattgca ccgccgtttg taccgcaaaa
     3721 tcaattaact catccacaaa ggtttttttg cctttttgtc gaggggcttc tgactctacc
     3781 accatgtctt tgccgtcaat gcgtgaatag gctgttaaga ctttgagtgc ggcggcataa
     3841 cctgccattt gcaagtcggc atcgttatat aaaccttctg agccttgctc tcgaactgag
     3901 ttatctaaac cgattaacga ggcgacttgt tcttttacgg cttcttctat ttcccagcct
     3961 aagtcatctc taaaggtttc taatgctttg tggcgtttgc gtagcaccag cataatggta
     4021 cctgtgacgt ttgcgccttg acgcaatgct gagtcggttt cggtgacgac ataccatgcg
     4081 gcagtgactt gcaaaccact cgcccaaatg atgcttgcca tgtctgccca aatagagccg
     4141 ctttggtggg taaacattaa gacttgtatg ccgttgtcgg gcattttttg ggtcattttg
     4201 cgataggcgg caaccatgcc ctgacggaag ccttcgtctt cgcctttgat ggccagtgag
     4261 cggcggctgt cccatgtcca atgagcaaat tccgcagggg gatttttacg caaccatgca
     4321 ataaaaaatt cggtgatttc ttcatatttc accgcatcgc cataaggtgg gtcggtgatg
     4381 taaatgtcgt tttcggtttc gagattttga gctggatgtg aatttatttc tgcatttcca
     4441 gcaatcggaa attctttaac gttcaaatca aatagccctt gtatgtagcc
//
LOCUS       DAOQMB010000174         4486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9290_length_4486_cov_12.642067, whole genome shotgun sequence.
ACCESSION   DAOQMB010000174 DAOQMB010000000
VERSION     DAOQMB010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9290_length_4486_cov_12.642067"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2054)
                     /locus_tag="PLY05_06810"
     CDS             complement(<1..2054)
                     /locus_tag="PLY05_06810"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF004883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HNC03051.1"
                     /translation="MMPSIYTAEDALFLSPDLLGGKAANLAWMTREGFPVPRWWVVTT
                     AAFKQLLNENGLSDWIEQSLTGFNHSEDLTALEPIAAAIREKILAATIPQSLTQELKQ
                     SFGQLENVYFAVRSSVLGEDAQGASFAGQMDSYLFQRGLTAIEESLLQVCASAFNTRA
                     LQYRLTKGIALTDIRAAVIIQEMIEGEVSGVMFTANPLTGNRKQCLISAAWGVGEGIV
                     AGICNTDEYTIGLYDNPIEQKLADKDLAVVFNHEQGRGTLEIHVEDNKRHIACLNNNQ
                     IQQLRDIGKAIAEHRRFPQDIEWAYRQRQFYILQTRPVTHLPAPTQPTDRRVVFDNSN
                     IQESYCGVTTPLTFSFANNAYRTVYEQTMRVMGAPEKQIQDHRDMLANLLGLVHGRIY
                     YNINNWYRGLLLLPAFKTNKADMERMMGLTDPVDLVQDTQLTTGQKLRKLPQVLLALW
                     RLMRGFKKMPFLVQQFRQMFDTVYHNIPRKELHTFTTGELVEKAHYLDRELLQKWTTP
                     IINDFYVMMMNGNVHRTLVAAGFEQPAVLQNNLLSGEEGIESTEPTKMLLRMCSYIRQ
                     QPHLRQVIEAGDNKNLLERVQVHDANFYQQCQEYIEKYGDRTIGELKLESITLRQDAS
                     FLFAILKNYLSRDDLTLETLSANEARFRQEAEQQAFTAIAEKLGQRRLRQFKTHLAKL
                     RDA"
     assembly_gap    2165..2264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2265..4474)
                     /locus_tag="PLY05_06815"
     CDS             complement(<2265..4474)
                     /locus_tag="PLY05_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HNC03052.1"
                     /translation="MTSARPFIFSPHYLFGEEHHAKCSFSYQEQRLFAQPIDITKGQL
                     CIALDENSAVVPNVGSKLDFIQLTVLGRDFVLPCMYVQKVNATQFHATELHLTSESCE
                     QSNAIIWEISYLLRKMPALETEVFYNESTLPKVPARGLYTEEARQDRLDFVREHTAAK
                     LQEVSKITLDSRKLVSNIEALIGTVEIPVGVAGPLHIKGNHAGGLFYAPFATSEGALV
                     ASATRGATALSRSGGVTTRVLGQCMMRVPAFTFDNMNNALFFAEWVKDHFRDLAEEVT
                     KYSNYATLVKVDPHVMGRSVHVHFIYQTGDAAGQNMTTTCTWQACKWIMKSIKRFEGL
                     RIENFLIEANLSNDKKVTYQTFLKGRGIRVIAECLLTAESCEKILKVSPKQLVTAYQN
                     FVMGSIGAGMVGININVANVIGAMFTALGQDIACVHESSLAQLHIELTDDNNAYCTLT
                     LPSLVIGTVGGGTNLPQQRECLEMLGCAGPNKAHKLAEIIAGFCLALDISTLSAIAAD
                     HFARAHEKLGRNRPVNYLKLGDLDNNFFNLAANNIAPEAPLIVDAIQQKYESKGSSII
                     TELTSHKTNKLIGHFPFVLKTIEGEEFKVMTKIKPLDEEVIIMLNSMAGMCDSRLSQA
                     FNHFRHQIGFKGCHVKELAVMAQQDPRFTQHAPKVYGIIEDAEREAYVIVEEHLENVE
                     LLDSADDTREWSKQHIEAAIKGIAQVHSIWYGREQELLQQPWMIDYPTAQGMEE"
BASE COUNT         1300 a          916 c          883 g         1287 t
ORIGIN      
        1 gcatcgcgca atttggctaa atgcgtttta aactgacgta aacgacgttg acccagtttt
       61 tcggcaatgg cagtaaacgc ttgttgttcg gcttcttgac gaaatcgtgc ttcgttagcg
      121 gataaagttt ctaaggttaa gtcatcacga cttaaatagt tttttagaat agcaaataaa
      181 aagctggcat cttggcgtaa ggtaatcgac tctagtttga gctcaccaat ggttcgatca
      241 ccatattttt cgatgtattc ttggcattgc tgataaaaat tagcatcatg cacttgtacg
      301 cgttctaaca aatttttgtt gtcacccgct tcaataacct gacgtaaatg tggttgttgg
      361 cgaatgtaac tacacattct taacaacatt ttggtgggtt cggtactttc gataccttct
      421 tcaccagaca gcaaattatt ttgtaaaaca gcaggttgct caaagccagc agcaaccaag
      481 gtgcgatgga cgttgccatt catcatcatc acataaaagt cattaatgat cggtgttgtc
      541 catttttgca ataattcacg gtctaagtag tgggcttttt cgaccagttc acctgtggta
      601 aaggtatgta gttctttacg tggaatgttg tgataaacgg tgtcaaacat ttgtctaaat
      661 tgttgcacca aaaacggcat ttttttaaag ccacgcatta aacgccataa cgccaataac
      721 acttgtggca gtttacgtaa tttttggcct gtcgttaatt gggtatcttg caccaaatca
      781 acagggtcag tcagacccat catgcgttcc atgtcggctt tattggtttt aaaggctggc
      841 aacaacaata aaccacgata ccaattgttg atgttgtaat aaattcgacc atgcaccaaa
      901 cctaacaaat tcgccaacat atcacgatgg tcttgaattt gtttttcggg tgcgcccatt
      961 acacgcatcg tttgttcata aacggtacga taggcattgt tggcaaaaga aaaggttaac
     1021 ggggtagtga caccacaata cgactcttga atattggaat tatcaaagac cacacgacgg
     1081 tctgtgggtt gggttggcgc aggcaaatgc gtgacaggcc gtgtttgtaa aatataaaat
     1141 tgcctttgac gatatgccca ttcaatatct tgcggaaaac gacgatgttc ggcaatggct
     1201 ttgccaatat cgcgtaattg ctggatttga ttgttattta aacacgcaat atggcgttta
     1261 ttgtcttcaa catgaatttc gagcgtgcca cgaccttgtt catggttaaa caccacagca
     1321 aggtctttgt cggcaagttt ttgctcaatc ggattatcat ataagccaat ggtgtattca
     1381 tcggtattac aaatacctgc aacgatgcct tcacccacac cccaagcagc actgattaaa
     1441 cattgtttac gattgcctgt taaggggtta gcggtaaaca tcacgccaga cacttcacct
     1501 tcaatcattt cttgaataat gacggcggca cgaatgtcgg ttaaggcaat acctttagtt
     1561 aaacggtatt gtaaggcgcg cgtattaaag gcactggcac aaacttgtaa taagctttct
     1621 tcaatggcgg ttaaaccacg ctgaaacaaa tagctatcca tttggcctgc aaaagacgca
     1681 ccttgggcat cttcacccaa caccgaagaa cgtactgcaa aatagacatt ctctaactga
     1741 ccaaaagact gtttaagctc ttgtgttaat gattgcggaa tggttgctgc taaaatcttt
     1801 tcacgaatgg cagcggcaat cggttctaag gcggttaaat cttcgctgtg attaaagcct
     1861 gttaagcttt gttcaatcca atcactgagg ccattttcgt ttaataattg tttaaacgcg
     1921 gcagttgtta ccacccacca acgtggcaca ggaaaaccct cacgggtcat ccatgccaag
     1981 ttagccgctt taccacctaa taaatcagga cttaaaaata aagcatcttc cgcagtataa
     2041 atagaaggca tcatggttgg ccaactcctt ctaattctaa taaattacgg aaggcacgat
     2101 agacacgttc cataaattca taatgacgga aggtgtgagc catcatatac atcggtaaac
     2161 gattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttttct tccatgcctt
     2281 gtgcggttgg gtaatcaatc atccacggtt gttgtaataa ctcttgctca cggccatacc
     2341 aaatactatg cacttgggcg atacctttaa tcgcagcctc gatgtgttgt ttgctccatt
     2401 cacgggtgtc atcagcgctg tctaataatt caacattttc caaatgttct tcaacaatga
     2461 cataagcttc acgttcggca tcttcaataa tgccatacac tttaggcgca tgttgggtaa
     2521 agcgggggtc ttgttgtgcc atcactgcca attctttgac atgacaacct ttaaaaccaa
     2581 tttggtggcg gaagtggtta aaggcttgtg ataagcgaga atcacacata cccgccatgc
     2641 tattgagcat gataattact tcttcatcta aaggcttgat tttggtcatc accttaaatt
     2701 cttcgccttc aatagttttt aacacaaaag gaaagtgacc aattaattta ttggttttgt
     2761 gcgaagttaa ctcagtgata atactagagc ctttggattc atatttttgt tgaatagcat
     2821 caacaattaa tggtgcttct ggcgcaatat tatttgcggc taaattaaag aagttattgt
     2881 ctaaatcgcc gagttttaaa tagttaacag gacgattacg acctagtttt tcgtgagcgc
     2941 gagcaaaatg gtcagcggca atagcagaca atgttgaaat atctaaggct aaacagaaac
     3001 cagcaataat ctcagccaac ttatgtgctt tgttagggcc tgcacaaccc aacatttcta
     3061 aacattcgcg ttgttgtggc aagttagtac caccaccaac tgtgccaatc actaaactgg
     3121 gtaaggtgag ggtacaatag gcattgttat catcggttaa ttcgatatgt aactgcgcta
     3181 aagacgactc atggacacaa gcaatatcct gacccaaagc ggtaaacatc gcaccaatca
     3241 cgttggcaac gttaatgtta atgccgacca tgcctgcacc aatcgacccc atgacaaagt
     3301 tttggtaggc agtaactaat tgtttagggc taactttaag gattttttcg caactttctg
     3361 cggttaataa acattcggca atcacacgaa taccacgacc ttttaaaaag gtttggtagg
     3421 tgactttttt gtcattcgat aagttggctt cgattaaaaa gttttcgatg cgtaagcctt
     3481 caaaacgttt gatggatttc ataatccatt tacacgcttg ccatgtgcaa gtggtggtca
     3541 tattttggcc agccgcatca cctgtttggt aaataaaatg cacatgcaca ctacgcccca
     3601 tgacatgagg atcaactttt actaaggtgg catagttaga atatttagtg acttcttcgg
     3661 ccaaatcacg gaaatgatct tttacccatt cagcaaagaa caaagcattg ttcatattgt
     3721 caaaggtaaa ggcaggcaca cgcatcatac attgacctaa gacgcgtgtg gtaacaccac
     3781 ctgaacgcga taaggcggtt gcgccgcgtg ttgcagaagc gactaatgcc ccttcagagg
     3841 tggcaaatgg tgcataaaac aaaccacctg catgattgcc tttaatatgc aaagggcctg
     3901 caacacccac tggaatttca accgtgccaa ttaaagcttc aatgttactg actaatttac
     3961 gagagtctaa agtgatttta gacacttctt gtaatttggc agcggtatgt tcacgaacaa
     4021 aatctaaacg atcttgacgg gcttcttcgg tatataaacc acgcgcaggc actttgggta
     4081 aggtactttc gttataaaat acttcggttt ctaaagcagg cattttgcgt aacagatagc
     4141 tgatttccca aataatggca ttgctttgtt cacaagactc actggttaaa tgcaattctg
     4201 tggcatgaaa ttgtgtggca ttgacttttt gtacatacat acaaggtaaa acaaaatcac
     4261 gacctaaaac tgttaattgg ataaaatcaa gtttgctacc cacattaggt acaacagcac
     4321 tattttcatc taaagcaata cacagttgac ctttagtaat atcaatcggt tgagcaaata
     4381 ggcgttgttc ttgatagcta aatgaacact tagcatgatg ctcttcacca aataaatagt
     4441 gaggagaaaa aataaatgga cgtgcgctag tcattataaa atcctc
//
LOCUS       DAOQMB010000175         4485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9295_length_4485_cov_15.749210, whole genome shotgun sequence.
ACCESSION   DAOQMB010000175 DAOQMB010000000
VERSION     DAOQMB010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9295_length_4485_cov_15.749210"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..727
                     /locus_tag="PLY05_06820"
     CDS             359..727
                     /locus_tag="PLY05_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03053.1"
                     /translation="MASNYIIAILAEEHDKALIKSLLATFGDRGDNQWAFTEDMSNAD
                     VVVVDFESHAQKLPLRGAKHGHIVVAYSQQAPSNPPTPFMLPKPVRGRDFVKLLERME
                     DVLTAHDEDEFAKTQRRIVF"
     gene            complement(787..2163)
                     /gene="nhaD"
                     /locus_tag="PLY05_06825"
     CDS             complement(787..2163)
                     /gene="nhaD"
                     /locus_tag="PLY05_06825"
                     /inference="COORDINATES: protein motif:HMM:NF038006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HNC03054.1"
                     /translation="MKTIASLLLLLPSFSFANTGQDLTNQPVGFMVLAIFVASYICVI
                     FEEKTHIKKSLPVMLAAGLIWIVIAANWTGETDAVEQAVKHYLLEYGELFLFLLAAMT
                     YVNAITERGIFEALRVWLIGKGFNYRRLFWLTGFCAFFLSPVLDNLTTALILCAVLMA
                     VGKDNPKFISLGAINIVVAANAGGAFSPFGDITTLMVWQKGLVEFWTFFNLFLPSLVN
                     FVVPAACMSFAISTDTPPASHEPSPLKRGAWIVLGLFICTIILAVSFHSFLHLPPFLG
                     MMTGLALLKFYGYYLHQTHIKKASDQAHYGNVGDVQAFDSFKFVAQAEWDTLLFFFGV
                     IMCVGGLGFVGYLELASNFLYHDLGATTANVSIGLLSAVVDNIPVMVAVLQMNPDMNT
                     GQWLLVTLTAGVGGSLLSVGSAAGVAMMGQAKGYYTFFSHLKWTWAVALGYVLSIACH
                     FLVNKALF"
     gene            complement(2240..2761)
                     /locus_tag="PLY05_06830"
     CDS             complement(2240..2761)
                     /locus_tag="PLY05_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03055.1"
                     /translation="MKKLLVTSALLAFSVSASALDLKQLGQQAVNQGVQNAVTGAIEG
                     KSGKEILKDTGEQTKQQTQQQIQQEAAEMAKKAPTADSNPMGTIATDIAAQKAAQKAG
                     GGEKGQLASDLVKGVSGAIQTKMTTPAPEAAPAATPTDANKAPAANASETAKAATNKP
                     KANKGKKKAKAKK"
     assembly_gap    2790..2889
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2890..3824)
                     /gene="hemW"
                     /locus_tag="PLY05_06835"
     CDS             complement(<2890..3824)
                     /gene="hemW"
                     /locus_tag="PLY05_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004656085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HNC03056.1"
                     /translation="MSSLPPLSLYIHVPWCVRKCPYCDFNSHTSPQSLPEKAYVAALI
                     ADLQADLAWVQGRKIGSVFIGGGTPSLFSGQTYQDLFTQLRQHLDFSSDCEVTLEANP
                     ATVEHDNFEGYLVAGINRLSLGVQSFNPTHLQTLGRIHSQQDAFNAIQLAKTAGFSNF
                     NLDLMHGLPQQDLSQALEDIDYALSFKPTHLSWYQLTIEPNTAFYRQPPALPDDDILW
                     AIQEAGQQKLAQAGFQQYEVSAYSQQRPSKHNLNYWQFGDYLAIGAGAHGKVTTPEGI
                     FRYRKTRLPKDYLAAAPQQQARLGLEKIVTEDLYF"
     gene            3830..4288
                     /locus_tag="PLY05_06840"
     CDS             3830..4288
                     /locus_tag="PLY05_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HNC03057.1"
                     /translation="MAYWLMKSEPSVFGIDHLAQRQVALWDGVRNYQARNFLRQMKNG
                     DLAFFYHSNCKVAGIVGLMRIAKEGYDDPTQFDPEHKYYDPKSTLDKPRWTGVDVEFM
                     EKFQETLSLTELKAMPELADLALVRQGNRLSLMPVSEAQWQAILAKCQRA"
     gene            complement(4285..>4485)
                     /locus_tag="PLY05_06845"
     CDS             complement(4285..>4485)
                     /locus_tag="PLY05_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNC03058.1"
                     /translation="AEIATRSPDAVLAAKRVLNAMVSQPESDTLALEKRWQRRLLLGK
                     NFKIAGKRAKTPELDYVQREFD"
BASE COUNT         1327 a          841 c          889 g         1328 t
ORIGIN      
        1 attaaattta ctcttgaacg tgctttagag tttattgaag atgatgaatt ggttgaagtt
       61 acaccacagt cgattcgtat tcgtaaaaaa tatttaactg aaaacgaacg taaacgcatg
      121 ggtcgcgctt aatgactgaa acaaaggcct cttagaggcc ttttttttat atgtttgagt
      181 aattttgttt gttttttcgc aaaattaaag aatattactt gagtaaagta gagtttttat
      241 cttaaattaa tctagatttt cttttgctct ttttgttggt agtcaatgcc aaacggttga
      301 gtttactaaa atagagcaat ttaaagccaa tatctaaaaa aggaagagga tagagattat
      361 ggcatccaat tacataattg ctattttagc agaagagcac gacaaagctt tgattaaatc
      421 gctattagcc acttttggtg atcgtggcga taatcaatgg gcatttactg aagatatgtc
      481 taatgctgat gtggtggtgg tagattttga gtctcatgct caaaaattgc cattacgcgg
      541 tgcaaaacat ggtcatattg ttgttgctta ttcacaacaa gccccatcaa atccaccaac
      601 accatttatg ttgccaaagc ctgtgcgtgg tcgtgatttt gttaagttat tagaacgcat
      661 ggaagatgtt ttaacagctc atgatgaaga tgagtttgct aaaacccaac gccgtattgt
      721 gttttaattt tcttagaccc cctctaaatc ccccccttat cagggggaga cttaacttgg
      781 ttagctttaa aataacgctt tattcaccaa aaagtgacaa gctatactaa gtacataacc
      841 caaagcaaca gcccatgtcc attttaaatg gctaaaaaag gtgtaataac ctttggcttg
      901 tcccatcatc gccacaccag ccgcagagcc aaccgataat aaacttcccc ccacacccgc
      961 agttaacgtc actaataacc attgtcctgt attcatatcg ggattcattt gtaatacagc
     1021 aaccatcaca ggaatattgt ccaccacggc ggataataag ccaatagaga cgttggctgt
     1081 ggttgcacca agatcgtgat agagaaaatt agaagccaat tctaaataac ccacaaatcc
     1141 tagtcctccc acacacataa tcaccccaaa gaagaataac agcgtatccc actcggcttg
     1201 agcgacaaat ttaaagctat caaacgcttg tacatcacca acgttgccat aatgtgcttg
     1261 atctgaggct tttttgatgt gggtttgatg taggtaataa ccataaaatt tgagtaaagc
     1321 caaacctgtc atcattccca aaaaaggagg aagatgtaaa aagctatgaa aggataccgc
     1381 taaaataatg gtacagataa acaaacctaa aactatccat gcgccacgtt ttaaaggtga
     1441 tggttcgtgg ctagcaggcg gtgtatcggt actgatggca aaagacatac acgcggcagg
     1501 tacaacaaaa ttaaccaaac taggtaaaaa taaattaaaa aatgtccaaa attcgactaa
     1561 acctttttgc caaaccatta gcgtagtgat gtcaccaaag ggactaaacg caccgcctgc
     1621 attagcagcg acgactatat taattgcccc taaactaata aacttaggat tatctttacc
     1681 aacagccatt aaaactgcac ataaaattaa ggcagtggtt aaattgtcta aaacaggtga
     1741 taagaaaaaa gcacaaaaac ctgttaacca aaataaacgt cgataattaa agcctttacc
     1801 aattagccaa actcgtaatg cttcaaaaat gcctcgttca gtaatagcgt tcacataggt
     1861 cattgctgct aataaaaata aaaatagctc accatattct aataaataat gtttaacggc
     1921 ttgttcaaca gcatcggttt cgcctgtcca attagcagca atgacaatcc aaattaagcc
     1981 agcggccaac atgacaggta acgatttttt aatatgcgtt ttttcttcaa aaatcacaca
     2041 aatataagaa gcaacaaaga tagctaatac cataaaacca acaggttgat tggttaaatc
     2101 ctgtccagta ttggcaaagc taaaagaggg gagtagtagt aataaactag cgattgtttt
     2161 caaaatttac actccatgat agtttttatg atcatggagt gtatgccgat tttttgttaa
     2221 aaataaatca atttaagatt tattttttgg ctttagcttt ctttttgcct ttattcgctt
     2281 ttggtttgtt agtagctgct ttagcggttt ctgatgcatt agcagcaggg gctttattgg
     2341 catcagtagg tgtagcagca ggggctgctt cgggggctgg tgtcgtcatt ttagtttgaa
     2401 tagccccact gactcctttt actaaatcac tggctaattg gcctttttca ccaccaccag
     2461 ctttttgagc cgccttttga gcggcaatgt cagtagcaat cgtacccatt gggttactgt
     2521 cggcagttgg agcttttttg gccatttcag ccgcttcttg ctgaatttgc tgttgagttt
     2581 gttgtttggt ttgctcacct gtatctttta aaatttcttt gccacttttg ccttcaattg
     2641 cgccagtcac cgcgttttgt acgccttgat taactgcttg ttggccgagt tgttttaaat
     2701 ctaaagcaga tgcagaaaca gaaaatgcta ataatgcact ggtaactaat aattttttca
     2761 tgagagcctc gttgttgtaa attaggactn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnt caaaatataa atcttcagtg acgatttttt ctaagcctaa acgcgcttgt
     2941 tgttggggag cagcggctaa atagtctttg ggtaaacgcg ttttgcgata cctaaaaata
     3001 ccttcaggtg ttgtgacttt gccatgtgcg cctgcgccaa tggctaaata atcaccaaat
     3061 tgccaatagt ttaaattatg tttgctcgga cgttgttggc tataagccga cacttcatat
     3121 tgttgaaaac ctgcttgagc caatttttgt tggccagctt cttgaattgc ccacaaaata
     3181 tcatcatcag gcagggcagg tggttgacga taaaaagctg tatttggctc aatggttagt
     3241 tgataccatg ataaatgtgt cggcttaaaa ctgagcgcgt agtctatatc ttcgagggct
     3301 tggcttaaat cttgttgggg taagccgtgc attaaatcta aattaaaatt gctaaaacct
     3361 gctgttttgg ccaattggat ggcattaaaa gcatcttgtt gactgtgaat tctgcctaaa
     3421 gtttgtaaat gtgttgggtt aaagctttga actcccaacg acaggcgatt gatgcctgcc
     3481 actaaatagc cttcaaaatt atcatgctct actgtagcag gattggcttc aagagtcact
     3541 tcgcaatcgc tgctaaagtc tagatgttgg cgtaattgcg taaataaatc ttgataggtt
     3601 tggcctgaaa ataacgatgg tgtgccacca ccaataaaca cactaccaat ttttcgccct
     3661 tgcacccatg ctaaatcggc ttgtaaatca gcaattagcg ccgcgacata ggctttttcg
     3721 ggtaaacttt gcgggctggt gtgtgaatta aaatcacaat aggggcattt acgcacgcac
     3781 caaggcacat gaatatataa acttaacggt ggcaatgagg acataaataa tggcttattg
     3841 gttaatgaag tctgaaccca gtgtctttgg tattgaccat ttggcacagc gacaagtggc
     3901 attatgggat ggtgtgcgta attatcaggc acgtaacttt ttacggcaaa tgaaaaacgg
     3961 cgatttggcg tttttttatc attcaaattg taaggtggcg ggtattgtcg gtttaatgcg
     4021 tattgctaaa gaaggctatg acgacccgac tcagtttgat cctgaacata aatattatga
     4081 tccaaaatca accttagaca aaccgcgttg gacaggtgtg gatgttgagt ttatggaaaa
     4141 gtttcaggaa actttgtctt taaccgaatt aaaggcgatg cctgagttgg cagatttagc
     4201 tttagttcgt caaggcaatc gcttgtcatt aatgcctgtg agtgaagcac aatggcaagc
     4261 gattttggct aagtgtcagc gtgcttaatc aaattcacgc tgtacataat caagttctgg
     4321 cgttttggca cgtttaccag caattttaaa gtttttacct aataataagc gacgttgcca
     4381 acgtttttct aaggctaagg tgtcggattc tggttgggag accattgcat ttaaaacacg
     4441 tttggcggct aacactgcat caggcgaacg tgtagcgatt tcggc
//
LOCUS       DAOQMB010000176         4484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9298_length_4484_cov_16.171596, whole genome shotgun sequence.
ACCESSION   DAOQMB010000176 DAOQMB010000000
VERSION     DAOQMB010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9298_length_4484_cov_16.171596"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1184
                     /gene="thrS"
                     /locus_tag="PLY05_06850"
     CDS             <1..>1184
                     /gene="thrS"
                     /locus_tag="PLY05_06850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNC03059.1"
                     /translation="DVAANIGAGLAKAALAGKVNGQLVDTSYLITEDTHLSIVTEKDA
                     DGLEVLRHSCAHLLAMAVKEIFPSAQVTIGPVIEDGFYYDFAFERPFTTEDLAKIEKR
                     MEELAAKDTPVVRSLMSRPEAVKLFEEMGEQYKVEIISSINTDQDLSFYRQADFIDLC
                     RGPHVPSLGKLKAFKLMKVAGAYWRGDSNNAMLQRIYGTCWANKKDLQAYVTRLEEAA
                     KRDHRKLGKQLGLFHMQEEAPGMVFWHPNGWTTYQVLEQYMRQVQRENGYLEVKTPQV
                     VDRILWEKSGHWANYADNMFTTHSENRDYAVKPMNCPCHVQIFNQGLKSYRDLPLRLA
                     EFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIGSEAAAFIALTLQVYKDFGFD
                     DVA"
     assembly_gap    1185..1284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1285..1806
                     /gene="infC"
                     /locus_tag="PLY05_06855"
     CDS             <1285..1806
                     /gene="infC"
                     /locus_tag="PLY05_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011182684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNC03060.1"
                     /translation="KDAKQRAAINTEIRAKEVRLIDKDGGQVGVVSLREALTAAESVQ
                     LDLVEIVPNATPPVCRIMDYGKHVFEQKQKLKDAKKRQHQVQIKEIKLRPGTEEADYQ
                     VKLKALVRFLEDGDKAKITLRFRGREVAHQELGMKMLQRIEVDLSEHGTVEQHPKMEG
                     RMMSMLIGPKKKK"
     gene            1931..2128
                     /gene="rpmI"
                     /locus_tag="PLY05_06860"
     CDS             1931..2128
                     /gene="rpmI"
                     /locus_tag="PLY05_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNC03061.1"
                     /translation="MSAKIKTVRGAAKRFKKTANGFKRKQAFKRHILTKKSAKRIRQL
                     RGCTQVAAVDVPSVQRMLPNT"
     gene            2146..2502
                     /gene="rplT"
                     /locus_tag="PLY05_06865"
     CDS             2146..2502
                     /gene="rplT"
                     /locus_tag="PLY05_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNC03062.1"
                     /translation="MARVKRGVIAHRRHKKILDRAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQKKRQFRALWIARINAAARLNGLSYSRLINGLKKASVEVDRRVLADIAVHD
                     AVAFAALAQKAKASLA"
     gene            2606..3622
                     /gene="pheS"
                     /locus_tag="PLY05_06870"
     CDS             2606..3622
                     /gene="pheS"
                     /locus_tag="PLY05_06870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNC03063.1"
                     /translation="MTDLTVLVNDALAMINSAADAQALEQARVQFLGKKSQLTELSKG
                     LGALDPEARKEQGAKLNQVRDAINQALDARRQAFAAEALQRQLESERVDVTLAGRGTL
                     PAGLHPVTRVMNRITEFFTQAGFQVASGPEVEDDFHNFEALNIPSHHPARAMHDTFYF
                     DAHRLLRTHTSPVQIRVMEQQKPPIRIVCPGRVYRCDSDLTHSPMFHQVEGLLIDENV
                     SFAQLKSVIQNFLRVFFEKDLAVRFRPSYFPFTEPSAEVDIQCVMCDGHGCRVCKQTG
                     WLEVLGCGMVHPQVLKNCGIDSEKYSGFAFGMGVERFAMLRYGVNDLRLFFDNDLRFL
                     AQFK"
     gene            3681..4106
                     /locus_tag="PLY05_06875"
     CDS             3681..4106
                     /locus_tag="PLY05_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03064.1"
                     /translation="MSVAYYICADVPEADENHEFFMDMDGKALASVEEDVLEQLAQRA
                     NVKPLMQFFSMPQGEWDDYVDDIKDLLDEDEVFDPQELTWFSADEGLKTVLALIAEID
                     KTPQLLAQPEAVIDDLESMAAILSRLVEHGIRWHLAIDI"
     gene            4201..>4484
                     /gene="pheT"
                     /locus_tag="PLY05_06880"
     CDS             4201..>4484
                     /gene="pheT"
                     /locus_tag="PLY05_06880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004955715.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a tetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 2 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNC03065.1"
                     /translation="MQISESWLREWVNPSQLNTQQLGHQLTMAGLEVDALQPIAPAFT
                     GVLVGEVVAVEPHPQADRLRVTTVNVGQAENLQIVCGAANVRAGLKVPVAT"
BASE COUNT         1258 a          829 c         1020 g         1277 t
ORIGIN      
        1 gatgttgctg ccaatattgg tgcaggttta gccaaagctg ccttagcagg caaagtgaat
       61 ggccaattag tagatacttc ttatttaatt actgaagaca ctcatttaag tattgtcacc
      121 gaaaaagatg cagacggttt agaagtctta cgtcattctt gcgcccactt attagcaatg
      181 gccgtgaaag aaatatttcc cagtgcccaa gtgacgattg gcccagtgat cgaagatggt
      241 ttttattatg acttcgcgtt tgaacgtcct tttaccactg aagatctcgc caaaattgaa
      301 aaacggatgg aagagttagc ggctaaagat acacctgtgg ttcgctcctt aatgtcacgc
      361 ccagaggctg tgaaattatt tgaagagatg ggcgagcagt ataaagttga aatcattagc
      421 agcattaaca ctgatcaaga tttatctttc tatcgccaag ccgactttat cgacttgtgt
      481 cgtgggcctc atgtgccatc tttaggcaaa ctaaaagcct ttaaattgat gaaagtggca
      541 ggcgcttatt ggcgtggtga tagcaacaat gccatgttac agcgcattta tggtacttgt
      601 tgggcaaaca aaaaagacct acaagcctac gtcactcgtt tagaagaagc ggccaaacgt
      661 gaccatcgca aattgggtaa acaacttggt ttattccata tgcaagaaga agctccaggt
      721 atggtgtttt ggcatccgaa tggttggaca acctatcaag tgcttgagca atatatgcgt
      781 caagtacaac gcgaaaacgg ttatttagaa gtaaaaacgc ctcaagtggt tgaccgtatt
      841 ttgtgggaaa agtctggcca ttgggcaaac tatgccgaca atatgtttac cacacattcc
      901 gaaaaccgtg actatgcagt taagcccatg aactgtcctt gtcacgtaca gatttttaat
      961 caaggcttaa aatcttaccg tgatttgcct ttgcgtttag ctgagtttgg ctcttgtcat
     1021 cgtaatgagc cgtctggttc tttacatggc ttaatgcgcg tgcgtggttt tacccaagat
     1081 gatgcgcata ttttctgtac tgaagagcaa atcggtagtg aagcagcggc atttattgcc
     1141 ttaactttac aagtttacaa agattttggc tttgatgatg ttgcnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnaaagac gcgaaacagc gcgctgcgat taatacagaa
     1321 atccgtgcaa aagaagtccg tttaattgat aaagatggcg gacaagttgg tgtggtttcc
     1381 ttacgtgaag ccttaacagc agcagagtct gtgcaattag acttggtaga gattgtgcca
     1441 aatgccactc cgccagtatg tcgtattatg gattacggca agcacgtctt tgaacaaaag
     1501 caaaagttaa aagatgctaa aaagcgtcaa caccaagtac agattaaaga gattaagcta
     1561 cgtcctggta cagaagaagc tgattatcaa gtgaaattaa aagccttggt tcgcttctta
     1621 gaagacgggg ataaagccaa aattactttg cgtttccgtg gacgcgaagt tgctcaccaa
     1681 gagttaggca tgaaaatgct acaacgcatt gaagttgact taagtgagca tgggactgtt
     1741 gaacagcatc ccaaaatgga aggtcgtatg atgtctatgc tcataggacc gaagaaaaag
     1801 aaataattct ttggaattga tttcttaccc tcaggctaaa ttctgagatt aaagctcctc
     1861 taaagatttc tttggatggg cttggttttt tttaatttat gctaaaaggg ttcattttaa
     1921 cgaggtaatt atgtctgcca aaattaagac agttcgtggt gctgccaagc gcttcaaaaa
     1981 aacagcgaat ggctttaagc gtaaacaagc gtttaaacgt cacattctga ccaaaaaatc
     2041 tgctaagcgt attcgtcagt tacgtggttg tacacaggtt gctgctgtgg acgtgccatc
     2101 agtacaacgt atgttgccta acacctaatt tttggagatt aaagaatggc tcgtgtaaaa
     2161 cgtggtgtta ttgcccatcg tcgtcacaaa aaaatccttg accgtgctaa aggttactac
     2221 ggcgcacgtt ctcgtgtgta tcgtgttgct ttccaagctg ttatcaaagc tggtcaatac
     2281 gcttaccgtg accgtcgtca aaagaaacgt caattccgtg ctttatggat tgcccgtatc
     2341 aatgctgctg ctcgcttaaa tggtttgtct tacagccgtt taatcaatgg tctgaaaaaa
     2401 gcttctgttg aagttgaccg tcgcgtgtta gctgacattg cggtacatga tgctgttgcg
     2461 tttgctgctt tagctcaaaa agccaaagcc agtctcgcct aagcgttatt tatcaagagc
     2521 ctaaatttat atttaggctc gcaaaaaagg gggagggcta atggccactt cccctttttt
     2581 tatatttgtt ttgtaggaaa aaagaatgac tgacttaact gtgctagtga atgatgcact
     2641 ggcaatgatc aatagtgctg ccgatgccca agctttagag caagcacgag tacagttttt
     2701 gggcaaaaaa agtcaattaa ctgaattatc taaaggttta ggggcattag accccgaagc
     2761 gcgtaaagag caaggtgcta aattaaatca ggttcgtgat gccattaatc aagccttaga
     2821 tgcgcgtcgt caagccttcg cagcagaggc gttacagcgt caattagaat ccgagcgtgt
     2881 tgatgtaacc ttagctgggc gtggtacatt gcctgcagga ttgcatccag tcacacgggt
     2941 gatgaatcgt attactgagt tttttactca agcaggtttt caggtggcca gtggcccaga
     3001 agtcgaagat gactttcata attttgaagc attaaatatt ccctcgcatc atcctgcgcg
     3061 tgcgatgcat gatacctttt attttgatgc ccatcgtttg ttgcgtacgc atacctcacc
     3121 tgtacaaatt cgagtgatgg aacaacaaaa accgccaatt cgtattgttt gtccaggtcg
     3181 tgtctatcgt tgtgattctg acttaaccca ttcgccaatg ttccatcaag tggaaggctt
     3241 gttaattgat gaaaatgtca gttttgctca actaaaaagt gtgattcaaa actttttgcg
     3301 cgtgttcttt gaaaaggact tagctgtacg ttttcgccct tcatacttcc catttaccga
     3361 accatcggca gaagttgaca ttcaatgtgt gatgtgtgat ggtcatggtt gccgtgtctg
     3421 caaacaaaca ggttggttag aagtattagg ttgtggcatg gttcatcccc aagtgttaaa
     3481 aaattgcggt attgatagtg aaaaatacag cggttttgcc tttggtatgg gtgttgaacg
     3541 ttttgcgatg ttgcgttacg gcgtcaatga cttacgttta ttctttgata atgatttacg
     3601 gttcttagcg caatttaaat aattgtgtac ttatgcttaa tgcaaaaaga taagtactct
     3661 aggtaacgag gagaaattgc atgagtgtgg cttattatat ttgtgccgat gtgccagaag
     3721 ctgatgaaaa tcatgagttt tttatggata tggacggcaa agcgttagct agtgttgaag
     3781 aagatgtgtt agagcaattg gcgcagcgcg caaatgttaa accattaatg caatttttta
     3841 gtatgccaca aggtgagtgg gatgattatg tcgatgacat caaagatcta cttgatgaag
     3901 atgaagtctt tgacccacaa gaattaacat ggttctccgc agacgaaggc ttaaaaacgg
     3961 tattagcatt aattgccgaa attgataaaa ccccacaact tttggcacag ccagaagcgg
     4021 tcattgatga tttagaaagc atggccgcaa ttttaagtcg tttagttgaa catggcattc
     4081 gttggcattt agcgattgat atttaacctt cactctcttt ttctccgaca gcgatcagag
     4141 aatttcctat ccctgtggga gagggttgag gtgagggaaa agcaaattaa ggtatagata
     4201 atgcaaatta gtgaaagttg gctgagagag tgggtgaacc caagccagtt aaatactcaa
     4261 caattaggcc atcaattaac catggctggc ttagaagtcg acgccttaca acccattgca
     4321 cccgcattta caggtgtttt ggtgggtgaa gtagtggctg tagaaccaca tccacaagca
     4381 gatcgtttac gtgtgacgac tgtgaatgtt ggccaagcag aaaatttaca aatcgtgtgt
     4441 ggagcagcca atgtgcgtgc tggtttaaaa gtgccagttg ctac
//
LOCUS       DAOQMB010000177         4465 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9361_length_4465_cov_13.359410, whole genome shotgun sequence.
ACCESSION   DAOQMB010000177 DAOQMB010000000
VERSION     DAOQMB010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9361_length_4465_cov_13.359410"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLY05_06885"
     CDS             <1..1194
                     /locus_tag="PLY05_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HNC03066.1"
                     /translation="NCPFPSKSIAGVGVAFYVMAALCTHLKNLGWFNNRPMPRMSDFL
                     DIVALGTVADVVALDDNNRILVAQGLERIRAGQCREGIKALLAVAKRERETIQAEDLA
                     FAVAPRLNAAGRLDDMSHGIECLLADSPILAQQYAQELDDLNIERRSIEGDMQQQALA
                     TMQQLNFNEENLPPAIVLFDASWHQGVIGIVAGRLKEKFHRPSIVFAPVDEQSDLIKG
                     SARSISGIHIRDAIELVTVRHAGLVSYFGGHAMAAGMTIPRANFEAFQQAFSQVIAEQ
                     ATEEHLTATILTDGELAAQEINLELAQLLRHSGPWGQAFPEPIFTGEFELRDFTVLKE
                     KHLKWLLKHPVTGEDLEAIAFNVDMRQWSAQTQKLFLAYRLDVNTFRNKSRLQLKVDY
                     WRALS"
     gene            1234..1497
                     /locus_tag="PLY05_06890"
     CDS             1234..1497
                     /locus_tag="PLY05_06890"
                     /inference="COORDINATES: protein motif:HMM:NF020265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNC03067.1"
                     /translation="MQAINVGMREFRANLPHYLLKMGEPIAVTRHGTTIGYFIPSQVK
                     ENEQMLELSLEDAMLLVEALDNPVTANPKLQNAFKKYQQTQKF"
     gene            complement(1617..2036)
                     /locus_tag="PLY05_06895"
     CDS             complement(1617..2036)
                     /locus_tag="PLY05_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC03068.1"
                     /translation="MNNIETYVLDTHAWIWQLFNPAKLGQKARQLLEQADNNDGVHIV
                     IPAVVLAEVCMIAQKQRIANFKPEHIPLLITTIKQHPAYTLSTLDDKIILASQQFIDI
                     PDIFDRLIATEANLLNAQLVSCDSVIRASQVKHLLVW"
     gene            complement(2038..2265)
                     /locus_tag="PLY05_06900"
     CDS             complement(2038..2265)
                     /locus_tag="PLY05_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03069.1"
                     /translation="MSLENTIIARLHCLDDANLYKVLDFIEHIDTLAKKPPKMTGLFA
                     HLNTTDNEISAALDTAKLERESALNQSLDTL"
     assembly_gap    2326..2425
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2426..2974
                     /locus_tag="PLY05_06905"
     CDS             <2426..2974
                     /locus_tag="PLY05_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131667308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNC03070.1"
                     /translation="EGDYAFGWLRTETGVHRLVRKSPFDSGNGRHTSFAAVFVSPEVD
                     DNIEIEINPADVRTDTYRASGAGGQHINKTDSAVRLTHIPTGTVVACQNERSQHANRD
                     RAWKMLRARLYELEILKKNAAKQALEETKSENGWGSQIRSYVLDQSRVKDLRTSVEHS
                     RPDAVLDGDLDDFIQASLKMGL"
     gene            2991..>4465
                     /gene="lysS"
                     /locus_tag="PLY05_06910"
     CDS             2991..>4465
                     /gene="lysS"
                     /locus_tag="PLY05_06910"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002054306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNC03071.1"
                     /translation="MSNTPEIQVSENELIAQRQQKLAELRTKGQAFPNDFRRDSYAQN
                     LQDKFAANTKEELEAGEKQVVSVAGRIMLKRVMGKASFITVQDMTGRIQFYVAQNSIG
                     EDTYADFKTWDIGDIVGGQGYIFKTKTGELSIHIEQVRLLTKSLRPLPDKFHGLSDQE
                     MKYRQRYVDLIMSEETRNAFKVRSQVIDGIRRFLRGRDFMEVETPMMHVIPGGAAAKP
                     FATHHNALDMPLFLRIAPELYLKRLVVGGFERVFEINRNFRNEGVSTRHNPEFTMIEF
                     YQAYADYKDLMDLTEAMLKTVALEVLGKTEVDYQGEVYDFGAPFVRMSMVDAILHFNP
                     QYTREQLWDMAAATQIVEKDIKEKVKPTWGIGKLQTIIFEETVETKLRQPTFITEYPA
                     EVSPLARRNDDNPFITDRFEFFIGGREIANGFSELNDPIDQAERFMQQVAEKDAGDDE
                     AMHYDADYVRALEYGLPPTAGEGIGIDRLVMLFTNSPSIRDV"
BASE COUNT         1270 a          815 c         1007 g         1273 t
ORIGIN      
        1 aattgtcctt ttccgagtaa aagcattgca ggggtgggtg tggcttttta tgtcatggcg
       61 gccttgtgta cccatcttaa aaatctgggt tggtttaaca acagacctat gccccgaatg
      121 agcgattttt tggatattgt tgcgttggga acggtcgctg atgtggtggc tttggatgac
      181 aataaccgta ttttagtggc gcaaggacta gagcggatta gagcagggca atgtcgagaa
      241 gggattaaag ccttattggc agtggcaaaa cgtgaacgcg aaaccattca agccgaagat
      301 ttagcctttg ccgttgcacc acgcctgaat gccgcagggc gtttggatga tatgtcgcat
      361 ggtattgagt gtttgttggc ggattcaccg atattagctc aacaatatgc ccaagaatta
      421 gatgacttaa atattgagcg acgcagcatt gaaggggata tgcagcagca agccttagca
      481 accatgcaac aacttaattt taatgaagag aatttgccac ctgcgatagt cttatttgat
      541 gcttcttggc atcaaggtgt cattggcatt gtcgcagggc gtttaaaaga aaagtttcat
      601 cgaccttcga ttgtttttgc gcctgtcgat gagcagagcg atttaattaa aggctcggca
      661 cggtcaatta gcggtattca tattcgtgat gccattgagt tagtcacggt acgtcacgca
      721 ggattggtca gttattttgg tggtcatgcg atggccgcag gcatgactat tccacgtgcg
      781 aattttgagg cttttcaaca agcgtttagc caagtgattg ctgagcaagc caccgaagaa
      841 catttaaccg caaccatttt gaccgatggt gaattagctg cacaagaaat taatttggag
      901 ttagcacaat tattgcgcca cagtggcccg tgggggcaag cctttcctga gccgattttt
      961 acaggcgagt ttgaattgcg tgattttacg gtgttaaagg aaaaacactt aaaatggttg
     1021 ttaaaacatc ctgtgacggg tgaagattta gaagcgattg cgtttaatgt ggatatgcgt
     1081 caatggtcag cacaaactca aaagctattt ttggcctatc gtttggatgt gaataccttt
     1141 agaaataaaa gccgcttgca gttgaaagtg gattattggc gagcattgag ctagtatcaa
     1201 aaatagtatt tttgtgtaca cattggaggt attatgcaag ccattaacgt aggtatgcga
     1261 gaatttagag caaatttgcc acattattta ttaaaaatgg gtgaaccgat tgccgtgaca
     1321 cgacatggga caacgatagg ctattttatt cctagccaag taaaagagaa tgagcaaatg
     1381 ctagagttgt cattagaaga tgcgatgttg ttagtggaag cattagataa tcctgtaaca
     1441 gctaacccaa aactacaaaa tgcttttaaa aaatatcaac aaacacagaa attttagtat
     1501 gtattgacca tagcaaattt ttgaaaatat gtaaggatag aaaaaatatg aaaaaatttg
     1561 tttgcgcgct tttaccaccg acttgcgtaa gctgttaaat ggtcaaaaat aactttttac
     1621 caaaccagca aatgcttaac ttgactagcc cgaataactg aatcacaact tacaagctga
     1681 gcattgagca aattggcttc tgtcgcaatt aaacggtcaa aaatatcagg aatatcaata
     1741 aactgttgac tagccaaaat aattttgtca tcaagcgtac ttaatgtata agcaggatgc
     1801 tgtttaatgg tggtaatcaa caacggaata tgctcaggct taaagtttgc tatgcgctgc
     1861 ttttgagcaa tcatacacac ttcggccagc acaaccgcag ggataacaat atgtacgcca
     1921 tcgttattgt cggcttgctc aaggagttgt ctagcctttt gcccaagttt ggcaggatta
     1981 aataattgcc atatccaagc atgagtgtct aaaacataag tctcaatgtt gttcaattta
     2041 cagggtatcc aacgattgat ttaaagctga ttctctttct aatttagccg tatctaatgc
     2101 cgcactaatc tcattatcgg tggtgtttaa atgagcaaaa agccctgtca tttttggcgg
     2161 ttttttagct agggtgtcga tatgctcaat aaaatcaagc actttatata agttggcatc
     2221 atctaaacaa tgcaaacgcg ctattatcgt attctctaaa ctcatgttca tgtgcttgcc
     2281 tcaatgtttt attgatgaac aggtcgcttg ttttatcata gcaagnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnngaagg cgattatgct tttggttggt tgcgtaccga
     2461 aacaggcgtg catcgtttag tacgaaaatc cccctttgac tcaggcaatg gccgtcatac
     2521 ctcgtttgca gcagtgtttg tctcgcccga agtggacgac aatattgaaa ttgaaatcaa
     2581 tcctgccgat gtgcgcactg atacctatcg tgccagtggt gcaggtggtc agcatattaa
     2641 caaaacggac tctgcggtac gtttaacgca cattcccaca ggcacagtgg ttgcgtgtca
     2701 aaatgagcgt tcacaacatg ctaaccgtga ccgtgcatgg aaaatgttac gtgcgcgttt
     2761 atatgagtta gaaattttaa agaaaaatgc ggcaaaacaa gccctcgaag aaaccaaatc
     2821 tgaaaatggt tggggtagcc aaattcgttc ttatgtttta gaccaatcgc gtgttaaaga
     2881 tttacgcacc agtgtcgaac attcgcgtcc cgatgccgtg ttagacggcg atttagatga
     2941 ttttattcaa gcttcattaa aaatgggttt gtaattttag agaaaatacc atgagcaata
     3001 cgccagaaat tcaagtatct gaaaatgaat taattgccca gcgtcagcaa aaattagctg
     3061 aattacgcac caaaggccaa gccttcccca atgacttccg tcgtgatagt tatgcccaaa
     3121 atttacaaga taagtttgcg gctaacacga aagaagaatt agaagcaggc gaaaagcaag
     3181 tggtgtctgt ggctggccgt attatgctca aacgggtgat gggtaaagcc agctttatta
     3241 ctgtgcaaga tatgacaggt cgtatccagt tttatgttgc ccaaaacagt attggcgaag
     3301 acacctatgc cgatttcaaa acatgggata ttggcgatat tgtcggtggt caaggttata
     3361 tttttaaaac caaaacaggt gaattgtcga ttcatatcga acaagtgcgt ttgttaacca
     3421 agtctttacg tccattgcct gataaatttc atggtttatc tgaccaagaa atgaagtacc
     3481 gtcaacgtta tgttgactta attatgagcg aagaaacgcg caatgcgttt aaagtgcgtt
     3541 cacaagtgat tgatggtatt cgccgctttt tacgtggccg tgactttatg gaagtcgaaa
     3601 cgccaatgat gcacgtcatt cccggtggcg cagccgcaaa gccgtttgca acgcatcata
     3661 atgccttaga tatgccgttg tttttgcgta ttgcccctga gttatacctc aaacgtttag
     3721 tggttggcgg ttttgagcgt gtgtttgaaa ttaaccgtaa cttccgtaac gaaggtgttt
     3781 ctacacgtca taatccagaa tttactatga ttgagtttta tcaagcctat gccgattaca
     3841 aagacttgat ggacttaacc gaagcgatgt taaaaaccgt tgctttagaa gtattgggta
     3901 aaaccgaagt cgattatcaa ggcgaagtgt atgactttgg cgcgccgttt gtacgcatga
     3961 gcatggttga tgcgattttg cattttaatc ctcaatatac ccgtgagcaa ttatgggata
     4021 tggccgctgc gactcaaatt gttgaaaaag acattaaaga gaaagtgaag ccaacatggg
     4081 gtattggtaa attacaaacc attatttttg aagaaacggt tgaaaccaag ttacgtcaac
     4141 ccaccttcat tactgagtat cctgctgaag tatcaccttt ggcgcgtcgt aatgatgata
     4201 atccgtttat tactgaccgt tttgagttct ttattggtgg gcgtgaaatt gccaatggtt
     4261 tctcggagtt aaatgacccg attgaccaag ctgagcgttt tatgcagcaa gtggctgaaa
     4321 aggatgcggg tgatgacgaa gccatgcact atgatgctga ttatgtgcgt gccttagaat
     4381 atggtttacc accgaccgca ggtgagggca ttggtattga ccgtttggtg atgttgttta
     4441 ctaactcgcc atcgattcgt gatgt
//
LOCUS       DAOQMB010000178         4459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9387_length_4459_cov_16.906449, whole genome shotgun sequence.
ACCESSION   DAOQMB010000178 DAOQMB010000000
VERSION     DAOQMB010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9387_length_4459_cov_16.906449"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..3451
                     /locus_tag="PLY05_06915"
     CDS             65..3451
                     /locus_tag="PLY05_06915"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC03072.1"
                     /translation="MKSTNFEFLRPENNLLANLGGLAEAVLHLDPGSALTRLRGFAEE
                     LTKAIYKEELLPRMPQSSFYELIKSPVFEDCVSKSLIHQINFLRIQGNDTAHGGEGEL
                     RNAQIALGTAHQLAMYMGIKYYGKKKEHIPAFVDVKDPTATFNQLQKSVSSYEKELQK
                     QQEELERVMEQLERERIKNIEKIEAPAKPDQQKRQQQSQKVADSLQWSEAKTRALLLD
                     AMLLQAGWDVHNTEFVGQEVEVDFPENPSGKGYVDYVLWGDNGQPLAVIEAKKSGNTN
                     LQAGREQARLYADAFERMGYQRPIIFYSNGYETFIWDDHQYNTYRPVYGFYSKDSLDY
                     LIYQRHYRAAELEKFNPELSIADRPYQIEAIKTVAANFQNQRRKALIIQATGTGKTRV
                     AIALVELLLRTGWAKRALFLCDRKELRVQADDAFKQNLPSEPRCVIGETNKIDQSARI
                     YIATYPAMMNRFAQLDVGFFDLIIADESHRSIYNKYRDLFDYFDALQVGLTATPVKFI
                     SRNTFDMFGCETTDPTFEFGLDAAINNEPPYLVPFRVKDMTTDFLREGIHYNDLTDAQ
                     KRQLEEDLGTEEARNTTIAGKDIGRKIFSEDTDRIILENLINNGIKDETGSLVGKTII
                     FAQRQDHAEHLEKLFCKLYPQYGSKVCKVIHNAIPHVESLIKEFKKPNNDFRIAISVD
                     MLDTGIDVPEVVNLVFAKPVKSWVKFWQMIGRGTRLRPNLFGLGKHKSEFLIFDHYGN
                     FDFFEQEYQEPEDVSAKSLLQTTFEARLELAQAALKANHPSAFDTAIELLRADINDLP
                     DASIAVKRQLRVVHQLQQNDLLKKFDAKTQHVLENTIAQLMSTRVLQDKHATQLDKLI
                     ATIQRCLVEKASCLEDGRDQLLAEIDKLAVNIQAVRQKDAMIAEVRSADFWQNLTIDK
                     LEKARKELRSIMKYRQTGTGGLYAMPTTKTGDTGVCESEREVKIAGANEAMIYRRRLK
                     AILDNMIAVNPTLQKIRKGEPIAEIELRTLTSTILTNHPSVSLSVLNEFYGRTAEQLD
                     LTVREIIGLDPQAIEDHFKGFLHLHPTLTAQQVRFMNLLKNYIAQHGSIVIEKLYDAP
                     FTAVSHQGIDGVFTADDVGELISVLKPFIHDVSEATNATY"
     gene            3560..>4459
                     /locus_tag="PLY05_06920"
     CDS             3560..>4459
                     /locus_tag="PLY05_06920"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC03073.1"
                     /translation="MLTGKIRNDIDKLWEKFWTGGITNPLTVIEQISYLMFARMLDMQ
                     EEIAERKANRTGKDFERLFANTPEGQLLRWKNFKNLNGKELHKHLKQHVFPYFAKLGK
                     TAHGHDGNAIDGLGHIGEYMQDADLEIKNESVLVSAVEMVDALPLTQSDVKGDIYEYL
                     LSKLTTAGINGQFRTPRHIIDAMIELIDPQPTETVCDPACGTAGFLARTMEYLNRVHS
                     SEVGTFVDEDGNKHYSGDLLEPYRSHINSQMFWGFDFDTTMLRVSSMNMALHGVNGAN
                     ILYQDSLSKSIKEHFPQQEENFFD"
BASE COUNT         1411 a          799 c          984 g         1265 t
ORIGIN      
        1 aatcgtaata aaataaactt ggcacgccat tcgctatatg gctggtatga acagaatata
       61 ttttatgaaa tcgacaaact ttgaattctt gcgacccgag aataatctgc tggctaacct
      121 aggtggttta gctgaggcgg tactacatct cgatccagga agtgcattaa cacggctacg
      181 cggttttgct gaagaactga ctaaagctat ttataaagaa gagctgctgc cgcgtatgcc
      241 acagtctagc ttttatgaat taataaaaag cccagttttt gaagattgtg ttagcaagtc
      301 actcatccat caaattaact ttttgcgtat tcagggtaat gatactgcgc atggtggaga
      361 aggtgagttg cgtaatgcac aaatagcatt aggcacagcg catcaattag ctatgtatat
      421 gggtatcaaa tattatggca aaaagaagga gcatattcct gcttttgtcg atgtgaaaga
      481 tccaacagct acctttaacc aattacaaaa atcagtctct agttacgaga aagaattgca
      541 gaagcaacag gaagagcttg agcgtgtgat ggagcaactt gagcgtgagc gcattaaaaa
      601 catcgaaaaa atcgaagctc ctgcaaaacc agatcaacaa aaacgtcagc aacagagcca
      661 aaaagtcgct gatagcttgc aatggagcga ggcaaagact cgagccttat tgcttgatgc
      721 gatgttgtta caagcgggat gggatgttca caataccgag tttgtaggtc aggaggtaga
      781 ggtagacttt ccagaaaatc caagtggcaa aggttatgtg gattatgtgc tttggggaga
      841 taatggtcag ccacttgccg tgattgaagc aaaaaaatca ggcaacacca atcttcaagc
      901 agggcgtgag caagcgcgac tatatgctga tgcttttgaa cgtatgggat accaacgtcc
      961 aattattttc tattctaatg gctacgagac ttttatttgg gatgatcatc aatacaacac
     1021 ttatcgtcct gtgtatggtt tttatagtaa ggacagccta gattatttga tttatcaacg
     1081 ccattatcgt gctgctgaat tagaaaaatt taatccagag ttaagtattg ccgatcgtcc
     1141 ttaccaaata gaagccatta aaaccgttgc cgccaacttt caaaatcagc gtcgtaaggc
     1201 tcttatcatt caggcaacag gtacaggtaa aacacgtgtg gctattgctt tagttgagtt
     1261 actgttgcgt acaggttggg caaaacgtgc gctatttctt tgtgatagaa aagagttgcg
     1321 tgtacaggca gatgatgcct ttaagcaaaa cctaccttca gaaccacgtt gtgtgattgg
     1381 tgaaacgaat aaaattgatc aaagtgcgcg tatttatata gctacttatc ctgcgatgat
     1441 gaaccgcttt gctcaattag atgtaggatt ttttgatttg attattgcag atgaatcaca
     1501 tagaagtatt tataacaaat accgtgattt gtttgattat tttgatgctt tacaagtagg
     1561 attaacggca actcctgtca agtttattag ccgtaatacc tttgatatgt ttggctgcga
     1621 aactactgat ccaacttttg agtttggatt agatgcagcg attaataatg agccaccgta
     1681 tttggtaccg tttcgggtca aagatatgac caccgacttt ttacgagagg gtattcacta
     1741 caacgattta actgatgcac aaaaacgtca acttgaagaa gacttgggca cagaagaagc
     1801 tcgtaataca acaattgcag gtaaagatat tggccgtaaa atctttagtg aagatacgga
     1861 tcgtattatt ttggaaaacc taattaataa tgggatcaaa gatgaaacgg gttctttagt
     1921 tggtaaaact attatttttg cccagcgtca ggatcatgcc gaacatctcg aaaaattgtt
     1981 ttgtaagtta tatccacagt atggtagcaa agtgtgtaag gtaattcaca atgctattcc
     2041 tcatgtagaa agtctcatta aagagtttaa aaagcccaac aatgattttc gtattgccat
     2101 ttcggtggat atgctcgata caggtattga tgtgcccgag gtggttaatc ttgtttttgc
     2161 taaaccagtc aaaagctggg taaagttttg gcagatgatt ggtcgaggca cgcgtttgcg
     2221 tcctaatctt tttggcttgg gcaagcacaa aagcgagttt ttaatttttg atcattacgg
     2281 taatttcgat ttttttgaac aggaatatca agaaccagaa gatgttagtg ctaagtcttt
     2341 actacaaacc acgtttgaag cacgtcttga attagctcaa gcggctctaa aagcaaatca
     2401 tccttctgct tttgatacgg cgattgagtt gttgcgtgcg gatattaatg atttgccaga
     2461 tgcgagcatt gcggtaaaac gtcaattacg cgtggtacat caattacagc aaaatgattt
     2521 attaaaaaag tttgatgcaa aaacacagca tgtactggaa aatacaatag cgcagttaat
     2581 gtcaactcgt gtattacagg ataaacatgc aacgcagtta gataaattaa ttgcaacaat
     2641 acaacgctgt ttggtagaaa aagccagctg tttagaggat ggtcgagacc aattattggc
     2701 agagatcgat aaattagcag ttaatattca ggcggttcga caaaaagatg ccatgattgc
     2761 ggaagtgcgt agtgctgatt tttggcaaaa tcttacgatt gataagttgg aaaaagcgcg
     2821 taaagagttg cgaagcatta tgaaatatcg ccaaacaggt acaggtggat tatatgccat
     2881 gccaacaacc aaaacaggcg atacgggtgt gtgcgaaagc gaacgagagg taaaaattgc
     2941 aggggctaac gaagcaatga tttatcggcg caggttaaaa gccatattgg ataacatgat
     3001 tgcagttaat cccacactgc aaaaaatccg aaaaggtgaa ccaattgccg aaattgagtt
     3061 aagaacactg acttcaacca ttctcaccaa tcatcctagc gtcagcttga gtgtattgaa
     3121 tgagttttat ggtcgaacag cagaacagct cgatttaaca gtacgcgaaa ttattggctt
     3181 ggatccacaa gcgattgaag atcatttcaa aggtttttta catttgcatc caacactcac
     3241 agcccaacaa gtgcgcttta tgaatttatt aaaaaattat attgcccagc atggttctat
     3301 tgtcattgaa aagctatatg acgcgccttt tactgccgtc tcacaccaag gaattgatgg
     3361 ggtatttact gctgatgatg tgggtgagct gatttcggtt cttaagccat ttatccatga
     3421 cgtatcggaa gcaacaaacg caacttatta aagcatgtag gggtcactct gcaatgatta
     3481 gattcaaaca tttcatttat gctttgtgac aagcaacaat acatgagaaa caacacaaaa
     3541 ccattaagag agctatttaa tgctaacagg taaaattcgt aacgatatcg acaagctttg
     3601 ggaaaaattt tggacaggcg gtatcaccaa tccattaaca gtgattgagc aaattagcta
     3661 tttaatgttt gcacgtatgt tggatatgca agaagaaatt gccgagcgca aagccaaccg
     3721 tacaggtaaa gacttcgagc gtttgtttgc caatacaccc gaagggcagc tattgcgttg
     3781 gaagaatttt aaaaacttaa atggcaaaga gttacataaa catttaaaac aacatgtttt
     3841 tccttatttt gccaaactgg gtaaaaccgc acatggccac gatggtaatg ctattgatgg
     3901 attagggcat attggcgaat acatgcagga tgcagacctc gaaattaaaa atgagtcggt
     3961 acttgtgagt gcggttgaaa tggttgatgc gctaccgctg acgcaaagtg atgtaaaagg
     4021 tgatatttac gaatatctac ttagtaagtt gaccaccgca ggaatcaatg gccaatttcg
     4081 tacgccacgc cacattattg atgccatgat tgagctgatt gatccgcaac ccacagaaac
     4141 cgtatgcgac cctgcctgtg gtacagcggg gtttttggcg cgtacgatgg aatacctaaa
     4201 ccgcgttcat tctagcgagg taggtacctt tgtggatgag gatggtaaca aacattattc
     4261 tggtgatttg ttggagcctt accgtagcca cattaacagc caaatgtttt ggggctttga
     4321 ttttgatacc actatgctac gcgtttctag catgaacatg gcattacatg gtgtcaatgg
     4381 tgccaacatt ttgtatcaag atagccttag taagtctatt aaagagcatt ttcctcaaca
     4441 agaagaaaac ttctttgat
//
LOCUS       DAOQMB010000179         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9437_length_4444_cov_14.673730, whole genome shotgun sequence.
ACCESSION   DAOQMB010000179 DAOQMB010000000
VERSION     DAOQMB010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9437_length_4444_cov_14.673730"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..321
                     /locus_tag="PLY05_06925"
     CDS             64..321
                     /locus_tag="PLY05_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNC03074.1"
                     /translation="MAIEFAIQKWGNSAAIRLPAPLLSQLNLHLGDKFTAKICNEGLM
                     LIPVKKRYSLDELLAQCDTQAEPPQDLAVWLDTPAVGDEIW"
     gene            315..668
                     /locus_tag="PLY05_06930"
     CDS             315..668
                     /locus_tag="PLY05_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ChpB family
                     toxin"
                     /protein_id="HNC03075.1"
                     /translation="MVKRSVFERGDIIRVCLNPTSGRELQGDFRPALVLSPKKFNELG
                     MTLVAPITQGGDFSRFAGFAVTLSGAGTNTQGAILVNMIRMMDVTARKAEKVEVAPAA
                     IVEETLMRLEAILER"
     gene            complement(727..1203)
                     /gene="greA"
                     /locus_tag="PLY05_06935"
     CDS             complement(727..1203)
                     /gene="greA"
                     /locus_tag="PLY05_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNC03076.1"
                     /translation="MQRYPMTVAGADALRAELDRLKNVERPRITAAIAEARAHGDLKE
                     NAEYHAAREQQSFAEGRIKEIEAKLSNVQIIDITKMTNNGKVIFGATVDIENVETGEV
                     KTYQIVGEDEADIKLGKISSGSPIARGLMGKEEGDTVTITTPSGKVEYEISEVRYI"
     gene            complement(1206..4421)
                     /gene="carB"
                     /locus_tag="PLY05_06940"
     CDS             complement(1206..4421)
                     /gene="carB"
                     /locus_tag="PLY05_06940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNC03077.1"
                     /translation="MPKRQDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPSMADSTYIEPITWQTVAKIIEKERPDAVLPTMGGQTALNCALAL
                     DANGVLEKFGVELIGATKEAIEKAEDRKLFDQAMRKIGLECPRASIAETMEQALEIQS
                     RFGFPVIIRPSFTMGGSGGGIAYNREEFVEICERGFDLSPTHQLLIDESLIGWKEYEM
                     EVVRDKNDNCIIICSIENFDAMGVHTGDSITVAPAQTLTDKEYQIMRNASIAVLREIG
                     VETGGSNVQFGIDPKTGRMVVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LQNDITGGKTPASFEPSIDYVVTKVPRFTFEKFPQAEQRLTTQMKSVGEVMAIGRTFQ
                     ESVQKALRGLEVGADGFSPKLNLAAPESEDKLRHELKNPGPDRIWYIGDAFRAGLSVE
                     QVYEYSAVDPWFLVQIEDIIKAEAQVKQLGFAGCSRDVLWGLKRKGFSDSRLASLLGV
                     SEKQLRKKRWELGVYPTYKRVDTCAAEFATSTAYMYSSYEEECEAQPSQRDKIVVLGG
                     GPNRIGQGIEFDYCCVHAALAMREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLED
                     VLEIVRVEQPKGVIVQYGGQTPLKLARHLEAAGVPIIGTSPDAIDRAEDRERFQQMVE
                     RLNLRQPPNRTARNPEEGVRLAAEIGYPLVVRPSYVLGGRAMEIVYNEEELRRYMREA
                     VQVSNESPVLLDRFLDDAIEVDVDCVSDGETVVIGGIMQHIEQAGIHSGDSACSLPPY
                     ALDEQVQNVMRQQSIDMARELGVVGLMNVQFAVKNNDVYVLEVNPRASRTVPFVSKCI
                     GVSLAKVAARCMAGQSLPSQNFTKEIIPPYFSVKEAVFPFAKFPGVDPILGPEMKSTG
                     EVMGVGKTFGEAFHKAVLGSNDKLPTGGCAFISVRDSDKALLPQLGRDLVALGFSLVA
                     TGGSQKILAQAGVPCKRVNKVTEGRPHIVDMLKNGEISLIINTTEGKQAQQDSFSIRR
                     SALQGKVYYTTTISGASAVCQALNAKGELSVARLQDLHLEV"
BASE COUNT         1237 a         1068 c          907 g         1232 t
ORIGIN      
        1 cattgcccaa cccccaaaaa gtatctacaa tgaatataca aaccaattag gagtcacatc
       61 atcatggcaa tcgagtttgc tattcaaaaa tggggtaaca gcgcggccat tcgtttacct
      121 gcaccattac tctctcaatt aaacttacat ttaggcgata aattcaccgc caaaatatgt
      181 aatgaaggct taatgctgat tccagttaaa aaaagataca gcttagatga actcttagcg
      241 caatgtgata ctcaagcaga gccaccacaa gatttagccg tttggctaga cacccctgct
      301 gtcggtgatg aaatatggta aagcgtagcg tatttgaacg tggtgatatt attcgtgtgt
      361 gcttaaatcc aacatcaggc cgtgaattac aaggtgactt tcgccctgca ctggtacttt
      421 cgccaaaaaa atttaatgaa ttgggtatga cattagttgc cccgattaca caaggtggcg
      481 atttctcgcg ttttgcaggc tttgccgtca ccttgtcagg cgcaggcaca aacacgcaag
      541 gcgcgatttt ggttaatatg atacggatga tggacgtaac agcacgtaag gccgaaaaag
      601 ttgaagtagc acctgcggct attgttgagg aaacgttaat gcgtttagag gcgatattag
      661 aacgctaaat aaaaaagccc catcaatcgc aaaattgaca aggcttttta gagttagcaa
      721 aacaagttat atataacgta cttcactaat ttcgtattca actttgcctg acggtgtggt
      781 aatcgtcacg gtgtcgcctt cttctttgcc cattaaaccg cgtgcaatgg gcgaaccaga
      841 ggaaatttta cctaacttaa tatcagcttc gtcttcgcca acaatttgat aggttttaac
      901 ttcacctgtt tcgacgtttt caatatcaac cgttgcccca aaaattacct tgccattatt
      961 ggtcatcttg gtgatgtcaa taatttgcac attcgataat ttggcttcga tttctttaat
     1021 acgaccttct gcaaaacttt gttgttcacg tgccgcatga tattcggcgt tttcttttaa
     1081 atcgccatgt gcgcgtgctt cagcaatcgc cgcagtaatc cgcggacgct caacattttt
     1141 taatcggtcg agttcagcgc gtaaggcatc tgcccccgcc actgtcatcg gataacgttg
     1201 catgactaaa cctccaaatg caagtcttgt aaacgagcaa ccgataactc acctttggca
     1261 tttaaggctt ggcacaccgc actcgcaccg ctgatggtgg tggtgtaata cactttacct
     1321 tgtaaagcac tacggcgaat cgagaacgaa tcttgttgcg cttgtttgcc ttcagtggtg
     1381 ttgataatca aactaatttc gccatttttg agcatatcca caatgtgtgg gcggccttcg
     1441 gtaactttat tcacccgttt gcaaggtaca cctgcttgtg ccaagatttt ttgactgccg
     1501 cctgttgcta ccaagctaaa acctaaagcc actaaatcac gacctaattg gggcaacaag
     1561 gctttgtcgg aatcacgcac cgaaataaaa gcacaaccgc ctgtgggtaa tttatcgtta
     1621 gaacctaata cggctttgtg gaaagcttca ccaaaggttt tgccaacgcc catgacttcg
     1681 cctgttgatt tcatttctgg ccctaaaata gggtcaacac ctggaaattt agcaaacggg
     1741 aataccgctt ctttgacgct aaaataaggt ggaatgattt ctttagtaaa gttttggctt
     1801 ggtaagcttt gaccagccat acaacgtgca gccactttgg ctaatgacac accaatacac
     1861 ttcgacacaa acggcacagt acgtgaggca cgtgggttta cttctaaaac atacacatcg
     1921 ttgtttttaa ccgcgaattg tacgttcatt aaacccacaa cacccaactc acgcgccata
     1981 tcaatcgatt gttgacgcat cacgttttgt acttgctcat ctaaggcata tggtggcaaa
     2041 gaacacgcgg agtcgccaga gtgaatacct gcttgttcga tgtgctgcat aatgccgccg
     2101 ataactactg tttcgccatc agacacgcaa tccacgtcaa cttcaatggc atcatccaag
     2161 aaacggtcta ataatacggg ggattcatta gaaacttgta ccgcttcacg catataacga
     2221 cgtaattctt cttcgttata aacgatttcc atcgcacgac cacccaacac ataggaagga
     2281 cgtaccacta aaggataacc aatctcggcg gctaaacgta caccctcttc tgggttacgt
     2341 gcagtacggt ttggtggctg acgtaaattt aaacgctcaa ccatttgttg gaaacgttcg
     2401 cggtcttcgg cacggtcaat cgcatctggg ctagtaccaa taattggcac acccgcagct
     2461 tctaaatgac gagctaattt taagggtgtt tgaccaccgt attgcacaat cacgccttta
     2521 ggctgttcaa cacgaacaat ctctaacacg tcttcgaggg taactggctc aaagtacaaa
     2581 cggtcagaag tgtcatagtc ggtagaaacg gtttctgggt tacagttaac cataatcgtt
     2641 tcataaccat cttcacgcat cgctaaggct gcatgcacac aacaatagtc aaactcaata
     2701 ccttgaccaa tacggtttgg cccaccacct aacaccacaa ttttgtcacg ttgtgaaggc
     2761 tgtgcttcac actcttcttc atacgaagaa tacatatagg ctgtgctagt ggcaaattca
     2821 gcggcacagg tatcaacacg tttgtaagtt ggataaacac ccaattccca acgttttttg
     2881 cgtagttgct tttcgctaac gcctaataaa ctggctaaac ggctatcaga gaagccttta
     2941 cgttttaagc cccacaatac atcacggcta caacccgcaa aacctaactg tttgacttgg
     3001 gcttcagctt tgatgatgtc ttcgatttgc accaaaaacc acgggtcaac cgcagaatat
     3061 tcatacactt gttcgacgct taaccctgca cggaaggcat cgccaatgta ccaaatacgg
     3121 tcagggccag ggttttttaa ctcgtgacgg agtttgtctt cagattctgg cgcagctaaa
     3181 ttcaattttg gactaaaacc atctgcacca acttctaaac cacgtaaggc tttttgtacc
     3241 gactcttgga aagtacggcc aatcgccatc acttcaccca ccgacttcat ttgagtggtt
     3301 aagcgttgtt cggcttgtgg gaatttctcg aaggtaaatc taggtacttt ggtcactaca
     3361 taatcgatgg agggttcaaa agacgcaggc gttttgccgc ctgtaatgtc gttttgcaat
     3421 tcatctaagg tataacccac cgctaattta gcggccactt tggcaatggg gaaacctgtg
     3481 gctttggagg ctaaagcaga tgaacggcta acgcgtgggt tcatctcaat cacaaccata
     3541 cgacctgttt tagggtcaat accaaactga acgttagaac cacctgtttc tacgccaatc
     3601 tcacgcaata ccgcaatact ggcattacgc atgatttgat attctttgtc ggttaaggtt
     3661 tgtgcagggg caacggtaat cgagtcacct gtatgcacgc ccatggcatc aaagttttca
     3721 atcgaacaga taatgataca gttgtcgttt ttatctctca caacttccat ttcgtattct
     3781 ttccaaccaa tcaacgattc gtcgattaac aattgatgag tgggtgataa atcaaaacca
     3841 cgctcacaaa tttcgacaaa ttcttcacgg ttgtaagcaa taccaccgcc cgaaccaccc
     3901 atggtaaagg atggacgaat aatgactgga aaaccaaaac gtgattgaat ttctaaggct
     3961 tgttccatcg tttctgcaat agaggcgcgt ggacattcta agccaatttt acgcatcgct
     4021 tggtcgaata atttacggtc ttccgctttt tcaatcgctt ctttggttgc accaattaac
     4081 tcaacaccaa acttttctaa tacaccgtta gcatccaagg ctaaagcaca gtttaatgcg
     4141 gtttgaccac ccatggttgg caataccgca tctggacgct ctttttcgat aattttagca
     4201 accgtctgcc atgtgattgg ctcgatatag gttgaatcgg ccatgctagg gtcggtcata
     4261 atggtcgcag gattggagtt aaccaaaatg acgcgatagc cttcttcgcg cagagcttta
     4321 caggcttgtg cgccagaata gtcaaactca cacgcttggc cgatgacaat cggacctgca
     4381 ccaataatca gaatgctttt aaggtcttgc cgttttggca tcactctact ccaataaaac
     4441 gctt
//
LOCUS       DAOQMB010000180         4392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9612_length_4392_cov_16.253632, whole genome shotgun sequence.
ACCESSION   DAOQMB010000180 DAOQMB010000000
VERSION     DAOQMB010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9612_length_4392_cov_16.253632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="PLY05_06945"
     CDS             complement(<1..1283)
                     /locus_tag="PLY05_06945"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HNC03078.1"
                     /translation="MLFDRINTLLPYIKTGGHRINVPRDLKSITTINKDRECSPESLG
                     LSSSAIDKIWQSVEAYYKTGLHPAITLVIRYKGQIVMSRGLGYSHVGAAGESPDDGSK
                     LATADTPLCLFSGSKAISAMLIHKLAEEGKLNIHDRVSQYIPEYASHGKHLTTIHDLL
                     THKAGIRIMPIANPTPELMYDFDTVVKILAESPPVGTPKKQQAYHAVTAGYILGAIAQ
                     KASGETLPQLLQRVLAEPLGCEHFTFGMAEDRRHQAAVSLPTGLDKVPVISKMLHHML
                     GVSDRDIVSAINTPDAHEAVIPAANIYCSAEEVCRFYQMMLDGGLWQGKRVFETTTIN
                     NATRRGKLLFDHSAKAPMRYSAGFMRGEKLWTIFGANTADAFGHLGFINILCWADPAR
                     EISVAFLNTGKSLAPEGFIGFANVTSTISSVFKAK"
     gene            1397..>1641
                     /locus_tag="PLY05_06950"
     CDS             1397..>1641
                     /locus_tag="PLY05_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03079.1"
                     /translation="MLINHQQPHIPAYIAIILVEWALSKGFSQQQLAGCLALDSLQLL
                     YADNYRLAAEDYENLLNLLYGQGYVDLGLMIGTQVNVS"
     assembly_gap    1642..1741
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1742..2281
                     /locus_tag="PLY05_06955"
     CDS             <1742..2281
                     /locus_tag="PLY05_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC03080.1"
                     /translation="DFSPLFVHFIGQQPSYHQALQDYLRCPIYYQQADCQFSMDKRLL
                     SLPLKTANALTAKQLLILCQQLLAEQQHHVLRVDEIVQRVQQELRASPNYWPSMPEVA
                     KRLAMSERTLRRKLQAAQTDFQEQVEQVRQQLAKQLLANKRVTVEQAAAVLGYSEAAS
                     FRRAFHRWMGVSPQGYRQG"
     gene            2360..2962
                     /locus_tag="PLY05_06960"
     CDS             2360..2962
                     /locus_tag="PLY05_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03081.1"
                     /translation="MKSQDILLVLKLLAMEIRQQEDELYTLRPHDWWCGWHDDSALKT
                     ISIEWTYQQLSNSLGISASECHGAVSRCLQNQLLRLSRKTQRPIPIARNITEFCVHGL
                     KYMLPLEVGAVVRGIPTTYAAPVLVGQLLGAGDLPYVWPDGTGKVMGISLLPIHKSVT
                     KAVKNDPILYELLALVDSIRMGHAREAELAKNLFIQRVKI"
     gene            2959..3648
                     /locus_tag="PLY05_06965"
     CDS             2959..3648
                     /locus_tag="PLY05_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNC03082.1"
                     /translation="MNNQKVMIEMIDVVAQSLGDDLLEKVVFVGGCTTALLVDDVVTL
                     QHIRSTEDVDMIVEVLSKSEYQDLCQKLRQRGFKESVEDDVICRWRLGSMIVDVMPTD
                     QEILGFSNIWYPEAVEFAKSYQLPSNRIIRVVSPTYFLATKIEAFAGRGNNDFLASQD
                     IEDILSLIDGCSSLIADLHAANKKVKSAIAAAIQTFLNESDFEYQVQSVAQDRQHENL
                     LFERLIEISHL"
     gene            3751..4083
                     /locus_tag="PLY05_06970"
     CDS             3751..4083
                     /locus_tag="PLY05_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC03083.1"
                     /translation="MHKVLFLSLSVFVLSACGNKTSLQPITLSEQQQKLFEQSCKTCH
                     ANPASPAPQVGDKAAWDKRLEQGLPTLVQHAIQGFNAMPAGGLCTTCTPPDFEAMIVY
                     MATAQTQK"
     gene            4154..>4392
                     /locus_tag="PLY05_06975"
     CDS             4154..>4392
                     /locus_tag="PLY05_06975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNC03084.1"
                     /translation="MLNRRQFLSFSAASGASLALSACYKAPNHPVPASQNAQGHYIWQ
                     NWAGSEYAYPSKRLAPSTLEELKTALANSTAPIRCV"
BASE COUNT         1291 a          809 c          912 g         1280 t
ORIGIN      
        1 tacttagcct taaaaacaga actaatcgta ctggtcacgt tcgcaaaacc aataaatcct
       61 tcgggggcta aagatttgcc agtatttaaa aacgccaccg aaatctcacg tgcaggatca
      121 gcccaacaca aaatattaat gaagcctaaa tgaccaaagg cgtcagcagt atttgcacca
      181 aaaatcgtcc ataatttttc gccacgcata aaaccagcag aataacgcat cggtgctttg
      241 gcagagtggt caaacaataa tttaccacgt cgtgttgcat tgttaatcgt ggtggtttca
      301 aacacgcgtt taccttgcca caaaccgcca tctaacatca tttgataaaa acgacacact
      361 tcctcagcac tacaataaat attggccgca ggaataacgg cttcatgggc atctggtgtg
      421 ttgattgctg aaacaatatc acggtcactg acacccaaca tatgatgcag cattttgcta
      481 atcacaggca ctttatctaa acctgttggc aagctaaccg ctgcttgatg acgacgatct
      541 tcagccatgc caaaagtaaa gtgttcacaa cctaatggct cagctaaaac tcgttgtaat
      601 aactgtggca aagtttcgcc agacgctttt tgggcaattg cccccaaaat atagcctgct
      661 gttacggcat gataagcctg ttgttttttt ggtgtgccaa caggtggaga ttcggccaaa
      721 attttcacga ctgtatcaaa gtcatacatt aattcgggag ttggattggc aattggcatg
      781 atccgaatgc ctgccttgtg ggttaataaa tcatgaatcg tggttaaatg tttgccatga
      841 gacgcatatt cgggaatata ttggctcaca cgatcatgaa tattgagttt accttcttcg
      901 gctaatttat gaatgagcat agcactaatg gctttagagc cagaaaacaa acataatggt
      961 gtatcagcag tcgctaattt tgaaccatca tcaggcgatt cacccgctgc acccacgtgg
     1021 ctataaccca aaccacgact catgacgatt tggcctttat aacgaatgac taaggtaatt
     1081 gcaggatgta agccagtttt ataatacgct tcaacacttt gccaaatttt atcaatcgct
     1141 gatgacgata aacctaaact ttcaggtgaa cattcacggt ctttgttgat agtggtaata
     1201 ctttttaaat cgcgtggtac attaatgcgg tgaccacctg ttttaatata aggaagtaag
     1261 gtattaatac ggtcgaataa cataaagtcg tgtcctgtgg ttaaaaatgg acttgtgtta
     1321 gctatgtgct cataataaat caaaaataaa taaaagcctt gttaaacgat gactttcgcc
     1381 cattaggata gccaatatgc tcatcaatca tcaacaaccg catattcctg cttatattgc
     1441 cattattttg gttgaatggg cattaagtaa gggttttagt cagcaacagt tggcaggctg
     1501 cttagcctta gatagtttac aactgttata tgctgataat tatcgtttgg cggcagaaga
     1561 ttatgaaaat ttattaaatc tgttatatgg tcaaggttat gtagatttgg gtttaatgat
     1621 tggtacacaa gtgaatgtat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 ngatttttcg ccgttatttg tccattttat cgggcaacaa cccagttatc atcaagcctt
     1801 gcaagactat ttgcgctgtc caatttatta ccaacaagcc gattgccaat ttagcatgga
     1861 caaacgtttg ttgtccttac cattaaaaac ggccaatgca ctcaccgcca aacaattatt
     1921 aattttgtgc cagcaattat tagcagaaca acagcaccat gtattgcgtg ttgatgaaat
     1981 tgtccaacgt gtgcaacagg agttaagagc tagccccaat tattggccaa gtatgcccga
     2041 agttgccaaa cgtttggcca tgtcagagcg tacgttaaga cgtaagttac aagcggctca
     2101 aaccgatttt caagagcaag ttgagcaggt gaggcaacaa ttggctaaac agctattagc
     2161 caataaacgt gtgacggtag aacaagccgc tgcggtgttg ggctatagcg aagcagccag
     2221 ttttagacgt gcttttcatc gttggatggg ggtttcgcca caaggttatc ggcagggttg
     2281 atattttctt gactttttta tttttttgta tttaatttat tctcaattcg cgaacagaga
     2341 aaatgtgggt ttttcaatta tgaaaagtca agatattttg ttggttttaa agttgcttgc
     2401 tatggagata agacagcaag aagacgagtt atatactctt aggcctcatg attggtggtg
     2461 cggttggcat gatgatagtg cgttgaaaac aatctcaata gagtggacat atcaacaatt
     2521 atctaacagt cttggcatta gtgcatctga gtgtcatggt gcagtaagtc gatgtttaca
     2581 aaatcaatta ttgcgtcttt ctagaaaaac acaaagacca attcctattg caagaaatat
     2641 aactgagttt tgtgtgcatg gtttgaaata tatgttaccg cttgaggttg gagcagtggt
     2701 cagaggtatt ccaaccacgt atgctgcccc tgtgttagtc gggcaactac ttggtgcagg
     2761 cgatttgcct tatgtatggc ctgatggtac aggtaaagtc atgggaattt cattattgcc
     2821 aatacataag tcagtgacaa aagcagtaaa aaatgatccg attttatatg agctattagc
     2881 attagtagat tcaataagaa tggggcatgc tcgagaggca gaattggcga aaaatctctt
     2941 tatacagagg gtgaaaattt gaataatcaa aaagtcatga ttgagatgat tgatgttgtt
     3001 gctcaaagtt tgggtgatga tttattggag aaagttgtat ttgtgggtgg ctgtacaacg
     3061 gctttactgg ttgatgatgt ggtgacattg caacatattc gtagtactga agatgtggat
     3121 atgattgttg aagtattgtc taaatctgaa tatcaggatt tatgccaaaa attacgccaa
     3181 cgcggtttta aagaatctgt tgaagatgat gttatttgta gatggcgttt aggttcgatg
     3241 atcgttgacg tcatgccaac cgatcaagaa atattgggtt ttagtaatat ttggtatcct
     3301 gaagctgtag agtttgctaa gtcgtatcaa ttaccctcta atcgtattat tcgagttgtt
     3361 agtcctacct attttttggc aactaaaatt gaagcattcg caggcagggg aaataacgac
     3421 tttttagcga gtcaagatat tgaggatata ctaagtttga tagatggatg ttcttcacta
     3481 attgcagatt tgcacgcggc taataaaaaa gtgaaatcag ccattgcagc agccatacag
     3541 acttttttga atgaatcaga ttttgaatat caggtacagt cagttgctca agataggcaa
     3601 cacgaaaatc tcttatttga gcgtttaatc gaaattagtc atttataaaa tggccgaaaa
     3661 tgtccccttt gttgtccctg cctgtgctag tatcaaatga tgataattct tagtatataa
     3721 aaataattaa atctctctga ggttggcaat atgcacaaag ttttgttttt aagcttgtcc
     3781 gttttcgtgt tatcagcgtg tggtaataag accagtttac aacccatcac gttaagcgag
     3841 cagcaacaaa aactgtttga gcaaagctgc aaaacttgtc acgctaatcc ggctagtcca
     3901 gctccacaag taggcgataa agcggcatgg gacaaacgtt tagagcaagg cttacccact
     3961 ttagtacaac acgcgattca aggttttaat gccatgcccg caggaggctt gtgtactact
     4021 tgtacaccgc ccgattttga agccatgatt gtgtatatgg ctacggctca aactcaaaaa
     4081 taatatcccc ctccctaagt ctcccctaaa ttagggggag ggcatctaaa cagaggaacg
     4141 aggttaaaat atcatgttaa atcgtcgcca gtttttaagt tttagtgccg cgagtggagc
     4201 aagtttagcc ttatctgctt gttataaagc ccctaatcat cctgttcctg ccagccaaaa
     4261 tgcacaaggt cattatattt ggcaaaattg ggcaggcagt gaatatgctt atccgagcaa
     4321 acgtcttgcc cctagcaccc tcgaagaatt aaaaacagct ttagccaata gcacagcacc
     4381 cattcgttgt gt
//
LOCUS       DAOQMB010000181         4359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9726_length_4359_cov_16.804368, whole genome shotgun sequence.
ACCESSION   DAOQMB010000181 DAOQMB010000000
VERSION     DAOQMB010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9726_length_4359_cov_16.804368"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1151
                     /locus_tag="PLY05_06980"
     CDS             39..1151
                     /locus_tag="PLY05_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC03085.1"
                     /translation="MTATHWQQYKGQIGLWAMVLAVLWWWPEESTSKYSPSEPFIELS
                     SVDVVTVKNTRIAQDIAVTGTIAPVRQTILNARVLGEVKQVFIREGQSVKAGQVLVVQ
                     DDRDLVARLQQAEASLLTAKAELALAQQNLDRIKPLQQQNYASSNDLANAERQVDIRH
                     AQVKSAEVSVIQAKQQLADMAVRAPYAGTVSERLVDAGQSVAPNTPLLKLVDLSQVEL
                     VAQVAATEVAAIKVGQVIYFTADGYADQTFAGRITRINPVAKAGSRRVDVYALVNNQK
                     GLLRGGLFAKGYVRDDQAQQGVAVPFSAVQERQGQKVVHVIRDNRLVAQEVSVGRRDE
                     AKSLALVTGLQAGERVLLLPPLPQNEGRIVRFKDGR"
     gene            1153..4359
                     /locus_tag="PLY05_06985"
     CDS             1153..4359
                     /locus_tag="PLY05_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03086.1"
                     /translation="MWLTRLSIHNPVLATMMMLALMVLGIFSWQKLSVEEFPDIEYPY
                     VMIVTDYPAASPEAVELEITRKLEDSINTIAGVRRIISTSYEGRSRIAVEFDLGVPIT
                     TAVQDVRDKVAAVKVKFRPEIKDPLVERYRPESSPIMSIAFQAQNLSARELTTVVEQQ
                     VVRRLQSVTGVGKVEVVGGVRREIAIHLQPERMQALGVGVGEVLAALRSDSADLPSGT
                     VVNDVQEKVVQVEGRMIEPQDFARLVVAMRGQSPVYLEQVAEISDSEQEAQSLALVNG
                     QRAVSIDIVKMTGANTLAVVDALRAASDELRPSLPTGVRMLEVADSAKSVRASLKEVQ
                     STLIEGAILAVTVVFLFLGSWRSTVITSLTLPIALLGAVFGLYLCALTLNTMTLMALS
                     LCIGLLIDDAIVVRENIVRHAAMGKSSYHAAMDGTREIGLAVLATTLTIVVVFLPVAF
                     MGGIIGRFFYQFGVAVACAVLLSMFVSFTLDPMLSSIWHDPHEHKAPRTEDEKLNKFL
                     QRFEKHQQPKTLVGQYVEQFLTRFDVTLEKFGQQYARVIRWALNHRRETLIVAFSSLF
                     LAFFLAKFVGKEFVPEPDLSEISVKLATPVGSSLAYTQQKVEQIEAVIRSYDGVQATY
                     ATINSGMDAGKNKVGIRVRLVPKKERKVSQKELVSILRQRLSQMAGVEISSVAAAKES
                     ISGGLKPIQISLQGQDLNQLRTIAEEYQAKVSKIDGVVDLESSLKAARPAYAIKVDRE
                     KAADMGVSISQIGTALRPLLAGDAVTTWQDAQGENYDVRVQLSQTDRQNRDDIWALPL
                     TSSRLINANGQPEMVMLGEVARIDDAQGASQINRRNLYREVLFSANVQGRPAGDVGAD
                     MREVASQMQLPAGYRFQIQGQNKDMDESMGYAKTALILAVLFIYFVLGAQFNSFIHPL
                     TIMSSLPLSLIGVFLALFVCRSTLNIFSLIGIVMLMGLVTKNAILLVDFIQHSIKEGM
                     NREQAILVAARTRLRPILMTTAAMVVGMLPLALGLGEGSEQRAPMAHALIGGVLTSTI
                     LTLVVVPVLYTLFDDLSKVFKTKNTLSKATHSQGLEHK"
BASE COUNT         1168 a          868 c         1070 g         1253 t
ORIGIN      
        1 cgtattcctt attttgaaag tgggaggttg taaatacaat gacagcaaca cactggcagc
       61 aatataaagg acaaattggc ttatgggcga tggttttggc cgtattatgg tggtggccag
      121 aagaatcaac gagcaaatat agcccaagtg agccctttat cgagctaagc tcagtggatg
      181 tggtcacggt aaaaaacacc cgtatcgctc aagatattgc agtgacaggt acaattgccc
      241 ctgtacgtca aaccatctta aacgcccgtg tgttaggtga agtcaaacaa gtgtttattc
      301 gtgaggggca atcggtcaaa gcagggcaag tcttagtcgt ccaagatgat cgtgatttag
      361 ttgctcgttt acaacaggcc gaagcaagtt tgttaacggc taaagcggag ttagctttag
      421 ctcaacaaaa tttagaccgt attaagccgt tacaacaaca aaattatgcc tcaagcaacg
      481 atttagctaa tgcggaacgt caagtcgata ttcgccacgc acaagttaaa tcagccgaag
      541 tctcggttat tcaagccaaa caacaattag ccgatatggc agtgcgtgcc ccttatgcag
      601 gtacagtgtc tgaacgttta gtggatgcag ggcagagtgt tgcgcccaat acaccgttat
      661 taaaattagt tgatttaagt caagttgaat tggttgctca agtcgcagct accgaagttg
      721 cggcaattaa agttggtcaa gtgatttatt tcaccgcaga tggttatgcc gaccaaactt
      781 ttgcagggcg tatcacgcgc attaatcctg tcgccaaagc aggtagccgt cgggttgatg
      841 tttatgcctt agtcaataat caaaagggct tattacgcgg tggtttgttt gccaaaggtt
      901 atgtccgtga cgatcaagct caacaaggcg tcgctgtacc attctctgcg gtgcaagaac
      961 gtcaagggca aaaagtggtt catgtcattc gtgataatcg tttagtggct caagaagtga
     1021 gtgttggccg tcgtgatgaa gcaaaaagtt tagcactggt cacaggctta caagcgggtg
     1081 agcgcgtttt gttgttgcca cccttgccac aaaacgaagg acgtatcgtc cgttttaagg
     1141 acggtcgtta agatgtggtt aacccgttta tctattcata atcctgtttt agccaccatg
     1201 atgatgttgg cattaatggt attggggatt ttttcgtggc aaaaactcag tgttgaagag
     1261 tttcccgata ttgaataccc ctatgtgatg attgtcaccg attatccagc cgcgtcaccc
     1321 gaagccgttg aattagaaat tacccgcaaa ttagaagata gcatcaatac cattgcaggg
     1381 gtgcgtcgga ttatttctac ctcttatgaa ggccgttctc gcatcgctgt agagtttgat
     1441 ttaggcgtac ccatcaccac tgccgtacaa gatgtccgtg ataaagttgc cgcagtaaaa
     1501 gttaaatttc gtcccgaaat taaagaccca ttggttgagc gttatcgtcc cgaatcttcg
     1561 ccgattatgt cgattgcttt tcaggcacaa aacttatcag cacgtgaact aaccaccgtt
     1621 gttgaacaac aagttgtccg tcgtttacaa agtgtgactg gtgtgggtaa ggtggaagtc
     1681 gttggtggtg tacgtcgaga aatagccatt catttgcaac cagaacggat gcaagcgttg
     1741 ggtgttgggg taggtgaagt attagccgcg ttgcgtagtg atagtgccga tttaccgtct
     1801 ggtacagtgg ttaatgatgt acaagaaaaa gtagtccaag ttgaaggccg aatgattgaa
     1861 ccacaagact ttgcgcgttt agtggtagcg atgcgtggtc aatcacctgt gtatttagag
     1921 caagttgccg aaattagtga cagcgaacaa gaagcccagt ccttagcgct agtgaatggc
     1981 caacgagcag tcagtattga tattgtcaaa atgacgggtg ctaatacttt agccgtggtg
     2041 gatgctttgc gtgcagcctc tgatgaatta cgtcctagct taccaacagg cgtgagaatg
     2101 ttggaagtgg cggacagtgc caaatcggta cgtgcctctt taaaagaagt gcaatcgacc
     2161 ttaattgaag gggcgattct agcagtgacg gtggtatttc tctttttagg ctcatggcgt
     2221 tcaaccgtaa ttactagttt aaccttgcca atcgccttat taggtgcagt gtttggtttg
     2281 tatctgtgtg ccttaacgct gaataccatg accttaatgg cgttatcgtt gtgtattggc
     2341 ttgttaattg acgatgccat cgtagtacgt gaaaacattg tccgtcatgc agcaatgggc
     2401 aaaagctctt atcatgcggc tatggatggc acacgcgaaa ttggtttggc ggtgttagcg
     2461 actactttaa cgattgttgt cgtcttctta cctgtcgcct ttatgggcgg tattattggc
     2521 cgtttctttt atcaatttgg tgtggcagtt gcttgtgccg tattgctgtc aatgtttgtc
     2581 agttttaccc tcgacccgat gttgtcgagt atttggcacg atccacatga acacaaagcc
     2641 ccacgcaccg aagacgaaaa actcaataaa ttcttacaac gttttgagaa gcaccaacag
     2701 cctaaaacgc tggtggggca atatgtagag cagtttttaa cacgttttga tgtgacctta
     2761 gaaaaatttg gtcaacagta tgcccgtgtc attcgttggg cattaaatca tcgccgtgaa
     2821 accttaattg tggctttttc ttccttattt ttagcgttct ttttggcaaa gtttgtcggc
     2881 aaggagtttg tacctgagcc tgacttaagc gaaattagtg ttaaattagc tacacccgtt
     2941 ggatcatctt tagcctatac ccaacaaaaa gttgagcaaa tagaagcggt gattcgtagt
     3001 tatgacggtg tacaagccac ctatgccacc attaacagtg gcatggatgc gggcaaaaac
     3061 aaagtcggta ttcgtgtgcg tttagtgcct aaaaaagagc gtaaagtcag ccaaaaagaa
     3121 ttggtcagta ttttacgcca acggttaagc caaatggcag gggtggaaat tagttcggta
     3181 gctgctgcca aagaatcaat tagtggtggc ttaaaaccga ttcaaatttc cttgcaaggc
     3241 caagacctta atcaattacg tactattgcc gaagagtatc aagccaaagt cagcaaaatt
     3301 gacggtgtgg ttgatttaga aagctcactc aaagcggcac gtcctgccta tgccattaaa
     3361 gttgaccgtg aaaaagcggc agatatgggc gtatccatta gtcaaattgg tacagcgtta
     3421 cggcctttat tggcaggcga tgcggtcacc acttggcaag atgcccaagg tgaaaactat
     3481 gatgtgcgtg tacaactatc acaaacagac cgtcaaaacc gagatgatat ttgggcttta
     3541 cccttaacta gcagtcgttt aataaatgct aatggtcaac ctgaaatggt gatgttgggt
     3601 gaagtagcga gaattgatga tgcccaaggc gcatcacaaa ttaatcgccg taacttatac
     3661 cgtgaagtgt tgttctcggc gaatgttcaa ggccgtcctg cgggtgatgt cggggcagat
     3721 atgcgtgaag tagccagtca aatgcaattg ccagcagggt accgtttcca aattcaaggc
     3781 caaaacaaag acatggatga gtctatgggt tatgccaaaa ctgcgttaat tttggcggtg
     3841 ttgtttattt attttgtgtt gggtgcgcaa tttaatagtt ttattcatcc cttaacgatt
     3901 atgtcttcat tgcctttatc gttgattggt gtgtttttgg cgttgtttgt ttgtcggagc
     3961 acgctcaata ttttctcctt aatcggtatt gtcatgctga tgggcttggt gactaaaaat
     4021 gccatcttat tggtcgattt tattcagcat tccattaaag agggtatgaa ccgtgaacaa
     4081 gccattttag tggctgcacg cacccgttta cgtcccattt taatgaccac ggcggctatg
     4141 gtggttggca tgttgccttt agccttaggc ttaggtgaag gctcagaaca acgtgcgcca
     4201 atggctcatg ccttgattgg tggcgtgtta acctccacca ttttaacctt agtggtagtg
     4261 ccagttttat ataccttgtt tgatgattta agtaaggtgt ttaagacaaa aaatacgcta
     4321 agcaaagcca cacactctca aggtttggag cataaataa
//
LOCUS       DAOQMB010000182         4347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9768_length_4347_cov_7.145154, whole genome shotgun sequence.
ACCESSION   DAOQMB010000182 DAOQMB010000000
VERSION     DAOQMB010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9768_length_4347_cov_7.145154"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1983
                     /locus_tag="PLY05_06990"
     CDS             <1..>1983
                     /locus_tag="PLY05_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03087.1"
                     /translation="ETTSMSLIGADHSELPLTVTDFEQATTQAPEGAICAVLKLPLTW
                     LQNISVIDMPGLGSVSQRHEAYTRAQIQQSDAILYLIEPRGPNQSDLQMLQMISQYGK
                     RVKVLVTRWDEVEAAAQRGEKTPNLEQWANQIYQQTGLKVRLAPVSYEGIGKDDILEF
                     IDRAKEDIDLIRLNRFKAELTPILQNALGINNDQQQACQTQTEEQIRQLHSELIERKQ
                     ALLELKNQLYAQQQSEQQDIQTQSKQSATVQRQQLQNDLQVIAHDLDNEEAWQSFGHK
                     GGELVNNALATLAAHLCQQSQHYGKPNLPDVYTTQFNLRFPEPSAIEVDSFLEVSKLE
                     QLQQALLNKEQEAQQKQEQLQNFSQDTGVADEEDALRQLLVQRQYIQNQDIAHVVERT
                     GGSNVGSMLGRVFGEVADIGVMFLSPAAIGAKVASVVGKGAKSVNIAVSVKKVASATS
                     KGVKAIQANNKSKLTGVMPPEIVDKVGVLEKFSLAYWGERIGSWLDGAPNEYLVKDPQ
                     AVAEQQQALALLDSQMMQLRRELERKEDLANERQLTGWALEQNRQEQARIQADITRLT
                     QQVAERKQQALTEQQEQHQRMIMQYSERAMSQWLRSYDQQADSMQALLVAQVKQYWVN
                     YVEQTLADRIQELDNLQTQIQAAPQQKAQRLAQLQQ"
     assembly_gap    1984..2083
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2211..2783
                     /locus_tag="PLY05_06995"
     CDS             2211..2783
                     /locus_tag="PLY05_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03088.1"
                     /translation="MANKKSSFKSGYKSPFQNNQENNSQSLQESPSYSNHKLSEFLQH
                     TMGNLTEAYLLSLGELAALKETYLKERLAQVQYNDKQLKKDLEQAKKTLEVYKPQVTL
                     LGELQRSEKELKKELKQVQQEFERCKPQVHLLAELQRSERELKKELHQAKKTIQDYKD
                     KADKSASLATGSSEQILLAQLEALKRLKQK"
     gene            2811..4307
                     /locus_tag="PLY05_07000"
     CDS             2811..4307
                     /locus_tag="PLY05_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNC03089.1"
                     /translation="MNKAPYYFSLVTAALNRFSEIMQPNNTLKDGCLTARVVGEFSAG
                     KTRLLSELLKDDIPPALFPVSSIERQTKLQLEITYGETAELSLIEREEDYDEAIELQK
                     LTVFPSRDELSDNFLPETHRLRLSIPEQRLILPNGDGWGDEKIPMRLFLIDTPGWNSG
                     EDDIAEREASQVMTGYHNLGLVYVSDANRLDGNVNQQHLKDFLEVLQSADFLQQAHLL
                     FVVTHCPSKDQERLKTRATNHVLNLWQELGYDKESLSLYVMCVDFAEFETEQRAEFRE
                     EFWHNLLAPLGEPQPIPDPWINAIHHWPKEWDIRPKLQQAWQQINHAKATLSLAMTEG
                     QFVKGMNMSRLLGLSAEQVQEKVMNTWLKQLKIDSKESIAATFTYTCALQKQHPLEFW
                     WKNYWQARMQDTLVASQKFMIAFEQAVATINPKTEDLQQHFAEQLEVLYQQALESLDS
                     SFTRLVETAQCLVSETALEKVIATLLTLSLLESRYNQHYNNALQGASL"
BASE COUNT         1440 a          805 c          946 g         1056 t
ORIGIN      
        1 tgaaaccacc tccatgtcac tgattggtgc tgaccatagt gagttaccac ttaccgtcac
       61 agactttgag caggccacta cgcaagcccc tgaaggagct atctgtgcgg tgcttaaatt
      121 gccattaaca tggttgcaaa atatcagcgt gattgatatg cctggtttag gcagtgtatc
      181 gcaacgtcat gaggcctaca cacgcgcaca aatacagcaa agtgacgcta ttttatattt
      241 aattgaacca cgaggcccta atcaaagtga tttgcagatg ctgcaaatga ttagtcaata
      301 tggcaaacga gtaaaagtgc tagtcacccg ttgggatgaa gtggaagctg ctgcgcaacg
      361 tggcgaaaaa acacctaact tagagcaatg ggcaaatcaa atttatcaac aaacagggct
      421 aaaagtacgt ttggctcctg taagctacga aggtatagga aaagacgata ttttagaatt
      481 tatcgaccgt gctaaagagg atatagacct tatacgactt aatcgtttta aggccgaatt
      541 aactccgatt ttgcaaaatg ctttaggtat taataacgac caacaacaag cctgtcagac
      601 tcaaaccgaa gaacaaataa gacaattaca cagtgaattg attgagcgca aacaagcctt
      661 acttgagctg aaaaatcagc tttacgcgca acaacagtca gagcaacaag acattcagac
      721 acaaagcaaa caaagtgcga cagtccagcg acagcagttg caaaatgact tgcaagtgat
      781 tgctcatgac cttgacaacg aagaggcatg gcaatcgttt ggccataaag ggggagagct
      841 ggtaaataat gcgttggcaa cattagcagc gcatttgtgc cagcagtctc agcattacgg
      901 taagcctaac ttgcctgatg tttacacaac gcaatttaat ttacgctttc cagaaccttc
      961 tgccatcgaa gttgacagct ttttagaagt aagcaagtta gaacagctac agcaagcatt
     1021 acttaacaaa gagcaagaag cacagcaaaa gcaagaacaa ctgcaaaact tttctcaaga
     1081 cacaggtgtt gctgacgaag aagacgcatt acgccaactg cttgtacaac gtcaatacat
     1141 acaaaatcaa gacattgcgc acgttgttga acgtacaggc ggtagtaatg tgggttcgat
     1201 gttgggacgt gtttttggcg aagtggccga cattggcgtg atgtttttga gtcctgctgc
     1261 tattggagct aaagtagcct ctgttgtggg caagggggca aagagtgtca acattgctgt
     1321 gagtgttaaa aaagttgcca gtgcaactag caagggtgta aaagcaatac aggccaataa
     1381 taaatcaaaa cttacagggg ttatgcctcc cgaaattgtg gacaaagtag gggttttaga
     1441 aaaattttcg ttggcttatt ggggcgaaag aataggcagt tggttagatg gtgcgccaaa
     1501 tgagtatctt gttaaagacc ctcaggccgt tgccgagcaa cagcaagctt tggcattact
     1561 tgatagtcaa atgatgcagt tgcgccgaga attagaacgt aaagaggatt tggctaacga
     1621 acgacagcta acaggttggg cattagagca aaatcgccaa gaacaagcgc gcatacaagc
     1681 cgacataact cgcctaactc agcaagtggc tgagcgtaaa caacaagcgt tgactgagca
     1741 gcaagagcaa caccagcgca tgattatgca atacagcgaa cgtgccatga gtcaatggct
     1801 tagaagttat gaccaacagg cagatagtat gcaggccttg ttagttgcgc aggttaaaca
     1861 gtattgggta aattatgttg aacaaacatt agcggataga atacaagagc ttgacaattt
     1921 gcaaacacaa atacaagcag cacctcaaca aaaagcacaa agactagcgc aactgcaaca
     1981 agannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnggattgg ctaggttggt
     2101 ctatcaatgg ttttttgatg tcaaaactaa agagcgcagg tgactttgta gttaataaag
     2161 atagagttgc caaatatatt gatgtggatg tttaagcgtt aggagcttgt atggctaata
     2221 agaaaagttc ttttaaaagt gggtataaaa gcccttttca gaataatcaa gaaaataatt
     2281 cacagagtct gcaagaaagc ccttcttata gcaatcataa actgagtgag tttttacagc
     2341 atacgatggg taatttaaca gaggcttatt tattgagtct aggggagtta gctgcattaa
     2401 aagaaacata tctaaaagaa cggttggcac aagtacagta caacgataag caattaaaaa
     2461 aagaccttga gcaagcaaaa aaaacacttg aagtgtataa gccacaagtg acactgctag
     2521 gggaattaca acgcagcgaa aaggagttaa aaaaagaact caaacaagta cagcaagagt
     2581 ttgagcgatg caaaccgcaa gtacatttgt tagcagaatt acaacgtagc gaaagggagt
     2641 taaaaaaaga gcttcatcag gccaaaaaaa ctattcaaga ttataaggat aaagctgata
     2701 aatcagcatc tctagcaact gggtcgtcag agcaaatttt attggcacaa cttgaagcgt
     2761 tgaaacgtct aaagcaaaaa taatcactat caattttttt aggagtacac atgaacaaag
     2821 ccccatatta tttttcgttg gtgacagcgg ctctcaatcg cttttcagag ataatgcagc
     2881 caaataatac gcttaaagat ggttgtttaa cagctcgtgt tgtaggtgag ttttcggcag
     2941 gtaaaacgcg gctattgagt gaactattaa aagatgacat tccacctgct ttatttcctg
     3001 tcagctctat cgaacgtcaa accaaacttc aactcgaaat tacctacggt gaaactgctg
     3061 agttaagcct gattgagcgc gaagaagact atgacgaagc gattgaactg caaaaattga
     3121 cggtattccc ttcacgagat gaattgagcg ataatttttt gccagaaacg catcgtttac
     3181 gcctaagtat tcctgaacaa cgcttgatac ttcctaatgg tgatggctgg ggcgacgaga
     3241 aaatacccat gcgcctattt ttaattgata cgcctggttg gaactctggc gaggatgata
     3301 ttgctgagcg tgaggctagc caagtaatga caggctacca caatttgggc ttggtctatg
     3361 tgagtgatgc taaccgttta gatggtaatg ttaaccaaca gcatttgaaa gattttttag
     3421 aagtattaca aagcgcagac tttttacagc aagcacattt gttgtttgtt gtcacgcatt
     3481 gcccaagcaa agaccaagaa cgcctcaaaa cacgtgcgac aaaccatgtg ttgaatctgt
     3541 ggcaagaatt aggctatgac aaagaatcat taagcctata tgtgatgtgt gtggactttg
     3601 ctgaatttga aaccgagcag cgtgcagaat ttagagaaga gttttggcac aatttgttag
     3661 cccctttagg tgaacctcag cctatacccg acccgtggat aaatgctatt catcattggc
     3721 ctaaagaatg ggatattcgc ccaaaattac aacaggcttg gcaacaaata aatcatgcta
     3781 aagccacatt gtcacttgcg atgactgagg ggcagtttgt gaaaggcatg aatatgtcac
     3841 gtctgttagg tttatcggca gagcaagtgc aagaaaaagt catgaataca tggcttaaac
     3901 aactaaaaat tgatagtaaa gaatctatcg cagccacttt tacctataca tgcgccttac
     3961 aaaagcagca tccacttgaa ttttggtgga aaaactactg gcaagccaga atgcaagata
     4021 ctttagtagc aagccagaaa tttatgatag cttttgagca agcagtagca acaatcaatc
     4081 ccaaaacaga agatttacaa caacattttg cagagcagtt agaggtacta tatcagcaag
     4141 ccctagaaag tcttgactct agctttacgc gtttagttga aacagcacaa tgcttggtga
     4201 gtgaaacagc cttagaaaaa gtgatagcta ccttattaac actgtcattg ttagaaagtc
     4261 gttacaatca gcattataac aatgccttgc aaggagcatc actatgataa ttcaggactt
     4321 tttaaaaaat agtagtcaag aagttgt
//
LOCUS       DAOQMB010000183         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_9800_length_4340_cov_17.904317, whole genome shotgun sequence.
ACCESSION   DAOQMB010000183 DAOQMB010000000
VERSION     DAOQMB010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9800_length_4340_cov_17.904317"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2601
                     /locus_tag="PLY05_07005"
     CDS             <1..2601
                     /locus_tag="PLY05_07005"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNC03090.1"
                     /translation="TDEQRAIVEHYQGAAIVYAVAGAGKSTTMAHRVQHLVEQYHINP
                     TRILVCSFSRETVNDIKQKIESLGVQNTSCYTFNALGRRIVQHAIKLGYFPNFDESQI
                     EFRSSQLSMRALVELGKQLGQNFNQLDVNQEDLQTFISICKGNLAYADLLEAKLPIHC
                     QQWATQATNSNPYYLKAYKLYEQIRQQQNLLTFDDQLLLAWEALERFDEIRTWAKSSF
                     DFVLVDEFQDVNKVQAQIADILTEDHRNYMAIGDDDQCIYEWRGADVRFILDFKKRYH
                     ATEFIISDNFRCPAEVTLLAGQVIAKNTDRHLKDLVSQRGFGGNVQLKGFQYDSGVAH
                     YVVEEYKKYLAQGGNAADCMILVRSYSQTAMIEAKLIQEDLAYQIIGNPRFYERPEVK
                     VLFTYLGFARQEADYRKQQNGQLTEQYIRRFGEILRQPNRYLSQQWINEFLTRGQQQN
                     GSLVDFLANNLHLASNEASQKRLEKLAYTLKELSKQLEHDAGSVVSWLVNELDYFESI
                     KKNAAIPELGEERCQNIHALINYAQKKGTVLAFLAHLRRLHLEDEQGDNSKPRLQMMS
                     IHRAKGLEWPVVFVPCCAEEQLPSSHKDNLEEERRLLYVAMTRSKQHLHLLYAPQDKM
                     SSFLKTVQAEQVLKTATELKELLNNSANTVQPQQMIHLALAVKQYPLMRYLRCWWPAA
                     ADFKQHLWQQAELALAQQQSAQLQIGQLKTQISEQNSLEQQQKDLKSMERRLLLFKAR
                     HVTVLLNQTAADNIQQQRFYFELNDSSGVIAVLNQTGQKIGIVDKQASRFPLEEVLDW
                     SWLMATVTIGFRFSYLKRTMNVTLQLSDKLSTLKKQKELAVPLHLQYLASESFKEDMQ
                     QLMGVLNPIM"
     assembly_gap    2651..2750
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3050..>4340
                     /gene="hflX"
                     /locus_tag="PLY05_07010"
     CDS             3050..>4340
                     /gene="hflX"
                     /locus_tag="PLY05_07010"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNC03091.1"
                     /translation="MEFFERHQGGEKAILVHLELKQYSQDEDLSEFKLLALSAGAEIL
                     SIVTGSRQKPDAKFYAGSGKVDEIAQLVQATGAELVLFNHSLSPAQERNLERAFQCRV
                     LDRTGLILDIFAQRAQTFEGKLQVELAQLQHLSSRLVRGWTHLERQKGGIGLRGPGET
                     QLETDRRLLKNRVVMLSERLEKVRQTRHQGRASRQKAAIPTISLVGYTNAGKSTLFNR
                     LTKAEVYAADQLFATLDPTLRRIDIGSLGTVVLVDTVGFVRHLPHQLVEAFRATLEET
                     VEADLLLHVVDAASSERDIQITAVNEVLAEIGSESRILMIYNKIDCLESHPPRIDYDA
                     EGKPCAVWLSARDNLGIELLEQALAELLVEDLQNFTLQMPPQYAKLRAQLFALNTVQD
                     EQLNDDGSSQLKIQLSLTELDILLRRMGLKRSDVGLSD"
BASE COUNT         1321 a          761 c          904 g         1254 t
ORIGIN      
        1 actgatgaac aacgtgcaat tgttgaacat tatcaaggtg cagcgattgt ctatgccgtt
       61 gcaggcgcag gtaagtcaac aaccatggcg catcgtgtgc aacatctggt tgaacagtat
      121 catattaatc caacacggat cttagtttgt tcatttagtc gtgaaacggt taatgatatt
      181 aaacaaaaaa ttgaatcatt aggtgtacaa aataccagtt gttatacttt taatgcttta
      241 ggtcgacgaa ttgttcaaca cgccattaag ttaggttatt tccctaattt tgatgaaagc
      301 caaattgagt ttcgcagcag tcaattgtcg atgcgtgctt tagtggagtt gggtaaacag
      361 cttggccaaa actttaatca acttgatgtt aatcaagaag atttacaaac ttttatttct
      421 atttgcaaag gcaatttagc ttatgcagat ttgttagaag ccaagctacc cattcattgt
      481 cagcaatggg caacacaagc gacaaactcg aatccctatt acctcaaagc ttataaatta
      541 tatgagcaaa ttcgccaaca acaaaatttg cttacctttg atgaccaatt attattagcg
      601 tgggaagcct tagagcgttt tgatgagatt cgtacatggg caaaatcgag ctttgatttt
      661 gttttggttg atgagtttca ggatgttaat aaagtccaag cacaaatcgc cgatatttta
      721 accgaagatc atcgcaacta tatggcgatt ggtgatgatg accaatgtat ctatgaatgg
      781 cgtggggcgg atgttcgttt tattttagat tttaaaaagc gttaccatgc cacagagttt
      841 attatttccg acaattttcg ttgtccagca gaagtaactt tattggcagg ccaagtgatt
      901 gccaaaaata ccgatcgaca tctgaaagat ttagtttcac aacgtggttt tggtggcaac
      961 gttcaattaa aaggctttca atacgatagt ggtgtagctc attatgtggt tgaagaatat
     1021 aaaaaatatt tagcacaagg tggtaatgct gctgattgta tgatcttagt gcgctcttat
     1081 tctcaaacag cgatgattga ggccaaactg attcaagaag atttagccta tcaaattatt
     1141 ggcaatccgc gtttttatga acgtcctgaa gtcaaagttt tgtttaccta tttgggcttt
     1201 gcgcgtcaag aagccgatta tcgcaaacaa caaaatggcc agttaaccga acaatatatt
     1261 cgccgttttg gtgaaatttt acgtcagcca aaccgttatt tatcacaaca atggattaac
     1321 gaatttttaa cgcgtggtca gcaacaaaat ggctcgttgg tcgatttttt ggcgaataat
     1381 ttacacttgg ccagtaatga agccagtcaa aagcgtttag aaaaattagc atacacctta
     1441 aaggaattaa gcaagcaatt agagcatgat gccggctcgg tagtgtcttg gttggtaaac
     1501 gagttagatt attttgagtc cattaaaaag aatgccgcga ttccagaatt gggtgaagaa
     1561 cgctgtcaaa atattcatgc gttaattaac tacgcacaga aaaaaggcac tgttttagca
     1621 ttcttggcac atttacgccg tttgcattta gaagacgagc agggtgataa cagcaaaccc
     1681 cgcttgcaaa tgatgtctat tcatcgggct aaaggtttag agtggccagt tgtttttgtg
     1741 ccttgttgtg cggaagaaca attaccttct agccataaag ataacttaga ggaagaacga
     1801 cgcctgttgt atgtggcaat gacgcgtagc aaacagcact tgcatttatt atatgcgcct
     1861 caagataaaa tgagttcttt tttaaagaca gttcaagcag agcaagtctt aaaaaccgcc
     1921 accgaactca aagagctatt aaataattca gcaaatacgg tgcagcctca acaaatgatt
     1981 catttggcct tagcggttaa acaatatcca ctgatgagat atttgcgttg ttggtggcca
     2041 gccgcagccg attttaaaca gcatttgtgg caacaagctg aactcgcctt agctcagcag
     2101 caaagcgccc aactacaaat tggccaatta aaaacacaaa tatctgagca aaattcgcta
     2161 gagcaacaac aaaaagactt aaaaagtatg gagcggcgtt tattgttatt taaagcacgt
     2221 catgtcacgg tgttactcaa tcaaacggcg gctgataata ttcaacaaca acgcttttat
     2281 tttgagctta acgatagcag tggggttatt gccgttttaa atcagacagg gcaaaaaatt
     2341 ggcattgttg ataaacaagc ctctcgtttt cctttagagg aagttttaga ttggtcttgg
     2401 ttaatggcaa cggtcaccat tggttttcgt ttttcttatt taaaacgtac gatgaatgtc
     2461 acattgcaac tttccgataa actttctacg ctaaaaaaac aaaaagaatt agcagtgcca
     2521 ttgcatttgc aatatttggc ctcagagagt tttaaagaag atatgcaaca actgatggga
     2581 gtattaaatc ccattatgta ggaatagata tgctagaaga taatcaacat gacgaaggac
     2641 aacaacaaac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aatgaggtta
     2761 aacatcgggt cgcgtggcta tgtcggccat gtcataatca tgtccataac atgctaagta
     2821 ataagcaatt ggagctggaa tacaatacgc tagagtcatt agcaacacat ccagatattg
     2881 ccaaatttgc ccaatggatt agtaataaaa ctatctgtta aaacttacgc ggttatcgct
     2941 tatttgtttg catttcaagc gtttttaacg cttttatgct cacaaaacgc gctaattttg
     3001 gcaaaatgcg taagttatat ggtttaaaag tttaagagag aaacgattag tggaattttt
     3061 tgaacgacat caagggggtg aaaaagccat ccttgtgcat ttagagttaa aacagtattc
     3121 acaagatgaa gatttaagtg aatttaagtt attagcttta tcggcaggcg cggaaatttt
     3181 gtcgatagtg actggttcac ggcaaaagcc tgatgctaaa ttttatgcag gttcaggtaa
     3241 agtcgatgaa attgctcaat tagtgcaagc gacaggcgcg gaattggtat tgtttaatca
     3301 ttctttaagc cctgcacaag aacgtaattt agagcgcgcc tttcagtgtc gagttttgga
     3361 tagaactgga cttattttag atatttttgc tcaacgcgca cagacttttg aaggcaagct
     3421 acaggtcgaa cttgcccaat tacaacatct tagctcacgt ttggttcgtg gttggacaca
     3481 cttagaacgt caaaagggtg gtattggttt acgtgggcct ggtgaaacac aattagaaac
     3541 tgaccgccgt ttgctaaaaa atcgggtggt gatgttatct gaacgcttag agaaagtacg
     3601 acaaactcgc caccaaggcc gtgcttcacg ccaaaaagca gctattccca ctatttcttt
     3661 agtgggttat accaacgcag gcaaatcgac gttatttaac cgtttaacta aagcagaagt
     3721 ttatgctgcc gatcaattgt ttgccacctt agacccaacc ttaagacgca ttgatattgg
     3781 cagtttaggc acagtcgttt tagtcgatac ggtgggcttt gtgcgtcatt taccacacca
     3841 attggtagaa gcatttagag caaccttaga agaaacggtt gaagcagatt tattattgca
     3901 tgtggttgat gcggccagta gcgaacgcga cattcaaatt acggctgtga atgaggtttt
     3961 agccgaaatt ggcagtgaaa gtcgtatttt gatgatttat aacaaaattg attgtttaga
     4021 aagtcatcct ccacgtatag attatgatgc agaaggcaag ccatgtgcgg tgtggttatc
     4081 ggcacgtgat aatttaggta ttgagctgtt agagcaggca ttagctgaat tattagtcga
     4141 agatttgcaa aattttacac tacagatgcc ccctcagtat gccaaactac gtgcccaatt
     4201 atttgcgctc aataccgtgc aagatgaaca gcttaatgat gatggctcga gtcaattaaa
     4261 gattcaacta tcattaacag aattagatat tttgttgcgg cgtatgggtt taaaacgtag
     4321 tgatgtagga ctttctgacc
//
LOCUS       DAOQMB010000184         4284 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10024_length_4284_cov_17.207141, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000184 DAOQMB010000000
VERSION     DAOQMB010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10024_length_4284_cov_17.207141"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(185..610)
                     /gene="trxC"
                     /locus_tag="PLY05_07015"
     CDS             complement(185..610)
                     /gene="trxC"
                     /locus_tag="PLY05_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HNC03092.1"
                     /translation="MTNIHYVCPHCEAINRFDKQRLNQQPKCGKCHQLLLTGEPIELN
                     NNNFDKFISRNDLPVVVDFWASWCQPCQMMAPHFAKAAQTLNGQYVFAKVNTEEAQQI
                     AARYGIRSIPTLMVFESGNEKKRVAGAMSAPQLVQWLTA"
     gene            complement(603..1484)
                     /locus_tag="PLY05_07020"
     CDS             complement(603..1484)
                     /locus_tag="PLY05_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03093.1"
                     /translation="MIEPVEERHERGFNVNGRLIAARCWHDKEKKPLLALHGWLDNAA
                     SFDLLAPALDDYYIVALDFAGHGYSEHRPKGVRYHLIDHVDDVLAVVKQLGWTRFSIV
                     GHSMGAGIAALFAAAMPEYIEKLIMIEGLGPYTGPADKAVEYLRTSLVEWQDFDEAPR
                     VIPSFDVAVKARMNGLLPVGEQAAQLLCARGTKAVEGGLIWSTDKRLRLNSPTRFYEQ
                     QVCAYLAAITAPSLLIMAEKTLPFFNVEDYQRRVAAHPNLQLVKLAGGHHLHIDDNVE
                     QVISTVKRFLAGVTTDD"
     gene            complement(1484..1918)
                     /locus_tag="PLY05_07025"
     CDS             complement(1484..1918)
                     /locus_tag="PLY05_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004671119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC03094.1"
                     /translation="MKQQRIEIIYDFPQPVEKIFKWIGDHNNLGEIFFPFKVTRIKDG
                     QGDVNGVGSVRKLSAFPLIPVEETVTAFQPNQLIEYRVTSKLAPIKDHLGVMLFESYN
                     GGTRLRYTITFTGVVPLLGPALKVGLDQGIKRGLAKLAKKSL"
     gene            complement(1915..2352)
                     /locus_tag="PLY05_07030"
     CDS             complement(1915..2352)
                     /locus_tag="PLY05_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNC03095.1"
                     /translation="MWQEPYWLWAAFACLLLIVEMLTGTFFFLAMAAAAAGTTVVAYF
                     SPDYLSQWVAFAVLSVIALLVWKKIRPAHQPHHDAASALNNRTRHLIGRQAILTEPIS
                     NGVGRINVDDSWWKVTGEDLPVGTHVQVIAVHDMVLTVEKVQK"
     gene            complement(2356..3297)
                     /locus_tag="PLY05_07035"
     CDS             complement(2356..3297)
                     /locus_tag="PLY05_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNC03096.1"
                     /translation="MGSFVVFMFVGFAIVLAYKSIQQVPQGYEYTVESFGRYTKTLQP
                     GLHFLMPFVERVGAKLNMMEQVLDVPPQQVISRDNAMVTIDAVCFFQIIKAAQAAYEV
                     SNLEYAIRNLTMTNIRTVIGSLELDAMLSQRDQINAQLLSTIDQATNPWGVKITRVEI
                     KDITPPHDLVEAMASQMKAERTKRAQILDAEGHRQAEILRAEGDKQAKILKAEGDRQA
                     AFLESEARERQAQAEAQATLVVSQAIAQGDLQAVNYFVAQKYIEALGEIAKAPNNKLV
                     MMPLEASGVIGSIAGIKDLVTELKTEQTPTPQRSNLR"
     gene            complement(3440..4111)
                     /locus_tag="PLY05_07040"
     CDS             complement(3440..4111)
                     /locus_tag="PLY05_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03097.1"
                     /translation="MMPIVSFAAKGDDLEELRADSKRNNEWILVKKDQLKDITTWAKR
                     EDGKNIRSFKIEMIAQADLETIARVHFDVDNIKRWFWETKESKLLKKVNDKEYYYYQV
                     FNAPLTIPDRDAIIRVQVEPFNAKRGYMAMKLTFIAEFMPAQPGKVRVDALNYYLRFT
                     PIDKNTTRIEVDGYVDPGGITPAWAINFVQRTAPYTTMIGLARMLQLPYYREGKEALD
                     FTYME"
BASE COUNT         1306 a          967 c          743 g         1268 t
ORIGIN      
        1 gaatgaaaac gtgccaacat cactaaacag gctaaacctg tatcaatatt atttggcgtg
       61 ggtatggttg ttggattaag ggtttctgat gacacgaatg acttccgatg tttgtactta
      121 aaataaaaaa ggctctatat ttttagagcc atattatact tataaatccg cataaaatgt
      181 gaagttaagc tgttaaccat tgcactaatt gtggtgctga cattgcacct gcaacacgtt
      241 ttttctcatt ccctgattca aagaccatta atgtcggaat actgcgaatg ccataacgag
      301 ctgctatttg ctgcgcttct tcagtattga ctttggcaaa cacatattga ccatttaaag
      361 tttgtgctgc tttggcaaaa tgtggcgcca tcatttggca aggttgacac caacttgccc
      421 aaaaatcgac aaccacaggc aaatcattac gactaataaa cttatcaaaa ttattgttgt
      481 ttaattcaat aggctcgcct gttaataata attgatgaca tttaccacat ttgggctgtt
      541 gatttaaacg ctgtttgtca aaacgattga ttgcttcgca atgagggcaa acatagtgaa
      601 tattagtcat ctgtggtcac tcctgccaaa aagcgtttta ctgtactgat tacttgttca
      661 acattatcat caatatgtaa atgatggccg cctgctaatt ttacaagctg caaatttggg
      721 tgtgcagcca ctcgacgttg ataatcttca acattaaaaa acggtaaagt tttttcagcc
      781 atgattaata aggatggagc ggtaatcgcg gctaaataag cacacacttg ttgctcataa
      841 aagcgtgttg gtgagttaag tcgtaaacgt ttatcggttg accaaattaa accgccctcc
      901 actgctttag tgcctcgcgc acacaacaat tgtgcagctt gctcacccac aggtaacaaa
      961 ccattcatcc gtgctttaac ggcaacatca aatgagggaa tcactcgtgg tgcttcatca
     1021 aagtcttgcc actccactaa agaagtacgc aaatactcta ctgctttgtc ggcagggcct
     1081 gtataagggc ctaaaccctc aatcataatt aatttttcaa tatattcagg cattgccgcc
     1141 gcaaataagg cggcaatgcc tgcccccatc gaatgaccaa caatactaaa acgtgtccaa
     1201 ccgagttgtt taaccactgc taaaacatca tccacatgat cgataagatg ataacgcaca
     1261 cctttgggtc gatgttcact ataaccatgc ccagcaaaat ctaaggcaac aatataataa
     1321 tcatctaagg ctggagcgag taaatcaaaa ctcgcggcat tatctaacca accatgcaaa
     1381 gccaataacg gctttttttc tttatcatgc caacaacgtg cagcaattaa acgcccatta
     1441 acgttaaaac cacgctcatg tcgttcttcg acaggttcta tcattacaaa ctctttttcg
     1501 ctaatttggc taaaccgcgt ttaatacctt gatctaagcc aacttttaac gctggcccta
     1561 ataagggtac aacacctgta aaggtaatgg tgtaacgtaa acgtgtgcca ccattataag
     1621 actcaaataa catcacccct aaatggtctt taataggtgc taatttactg gtgacacgat
     1681 attcaattaa ttgattaggc tgaaaggccg tcacggtttc ttcaacagga attaagggaa
     1741 aagcactgag cttacgcacc gaacctacgc cattaacatc accttgacca tctttgatac
     1801 gagtcacttt aaagggaaag aaaatctcgc ctaagttgtt atgatcacca atccatttaa
     1861 aaatcttctc aacaggttgc ggaaaatcat aaataatttc aatacgttgt tgtttcattt
     1921 ttgcaccttc tcaacagtaa gaaccatatc gtgtacggca ataacttgta catgtgtacc
     1981 cacaggcaaa tcttcacctg ttaccttcca ccaactatca tccacattta ttcgaccaac
     2041 accattgctg attggctccg ttaaaatggc ttgtcggcca attaaatgcc gtgttcgatt
     2101 atttagagca ctcgctgcat catgatgcgg ctgatgtgct ggtcgtattt ttttccaaac
     2161 caagagggca atgacactta atactgcaaa tgccacccat tgactcaaat aatctggact
     2221 aaaataagca acgacggtcg tgcctgcggc tgcggctgcc atcgctaaaa agaaaaatgt
     2281 gccagtgagc atttccacaa tgagcaataa acaggcaaat gctgcccata accaataagg
     2341 ctcttgccac atagatcacc tcaaattaga acgctgtgga gtgggtgttt gctcagtttt
     2401 taactcagtg actaaatctt taatacctgc aattgagcca atcacgccag atgcctcaag
     2461 tggcatcatc actaatttat tgttaggagc tttggcaatt tcgcccaagg cttcaatata
     2521 tttttgtgcg acaaaataat tcaccgcctg caaatcacct tgagcaatcg cttgtgatac
     2581 cactaaagtc gcttgtgctt cggcttgtgc ttgacgctca cgtgcttctg actctaagaa
     2641 tgccgcttgt ctatcacctt ctgctttgag aattttggct tgtttatcac cttctgcgcg
     2701 taaaatttcg gcttgacgat ggccttcggc atctaaaatt tgtgcccgct tagtacgctc
     2761 tgctttcatt tgtgaagcca ttgcttcaac caagtcatgt ggcggggtaa tatctttaat
     2821 ttctacacgg gtaattttta ccccccaagg attggtagct tggtcgatgg tactcaataa
     2881 ttgagcatta atttgatcac gctgtgacaa catcgcatcc aactctaaag agccaatcac
     2941 tgtccgaata ttggtcatgg ttaaattgcg aattgcgtat tctaagttac tgacttcata
     3001 agctgcttga gccgctttaa taatttgaaa gaagcagacc gcatcaatcg taaccatcgc
     3061 attatcacgc gaaatcactt gttgtggtgg cacatctaaa acttgctcca tcatattcag
     3121 cttagcccca actctctcta caaatggcat caaaaaatgt aaacctggtt gcaatgtttt
     3181 tgtataacga ccaaaagact cgacggtata ttcataacct tgtggtactt gctgaatcga
     3241 cttatacgcc aaaacaatcg caaaaccaac aaacataaaa acgacgaagc ttcccatgtc
     3301 ttactccact tacttaaatc ttcagcatat gctttttatc attgcccatc ttgtaccttg
     3361 tttttgctct atgaagcaag caatagacaa cgaaaaaaca caaaaaaggc ctcaattgag
     3421 gcctttagaa tatgtaattt tactccatat atgtaaagtc tagtgcttcc tttccttctc
     3481 gataataagg taactgcaac atacgtgcta aaccaatcat cgttgtatag ggagcagtac
     3541 gctgaacaaa attaattgcc catgcaggtg taatcccacc aggatcaaca taaccatcaa
     3601 cttcaatacg tgtggtattt ttatcaatag gagtaaaacg tagataataa tttaaagcgt
     3661 ccaccctaac tttacctggc tgtgcaggca taaactccgc aataaaggtc aacttcatgg
     3721 ccatatatcc ccgtttggca ttaaacggtt caacttgcac acgaataatg gcatcacgat
     3781 caggaattgt taatggtgca ttaaagacct gataataata atattcttta tcattgacct
     3841 tttttagcaa ctttgattct ttggtttccc aaaaccaacg cttaatgtta tccacatcaa
     3901 aatgaacacg cgcaatcgtt tctaaatctg cttgtgcaat catttcaatt ttaaatgagc
     3961 gaatattttt accatcttcg cgttttgccc acgtggttat atcttttaac tgatccttct
     4021 ttactaaaat ccattcatta ttacgtttgc tatctgctct taattcctca agatcatcac
     4081 ctttagccgc aaatgaaaca atgggcatca tcaataaaga tgcacatata gaacttaata
     4141 atacagtttt ttttaaacac gaaatcgccc tctctatgat tcgatgttaa cctgaaattt
     4201 aattattagt gcatttactc taaaacgtca ttggccgtat cggacagata cccccccaaa
     4261 taagctaaaa agccccaaaa gggg
//
LOCUS       DAOQMB010000185         4283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10029_length_4283_cov_15.531220, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000185 DAOQMB010000000
VERSION     DAOQMB010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10029_length_4283_cov_15.531220"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLY05_07045"
     CDS             <1..795
                     /locus_tag="PLY05_07045"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNC03098.1"
                     /translation="DFEQGFMLLSDLGDTLLRPCLTEQTVDGLYQQAMREILVMQQSP
                     SPENYPIPPYDQTRLLFEMSLFKDWFVAQYLDLSLTPEEIKVIDDCCQQIATAIAEQP
                     TVFVHRDYHSRNLMLLANGKLGVIDFQDALMGAITYDLVSLLRDAYVEWPNQQVVAWV
                     EDFRQLLVAQGRLTTVSQPQFLTWFDWMGAQRHLKVLGIFARLSIRDGKHGYLNDIPL
                     VFKYLLNEIKDYPEFNALNQLLISKILPSYLAKNPQAAAMLGEFVQ"
     gene            792..1478
                     /locus_tag="PLY05_07050"
     CDS             792..1478
                     /locus_tag="PLY05_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNC03099.1"
                     /translation="MKAMILAAGLGNRMRPLTLTTPKPLLAVGGKPLIVWHIDALKAV
                     GITELVINTAWLGHKLHEALGDGAAYGVNIQWSDEQEPLETAGGIQQALPLLGNEPFL
                     LINGDIWLRYDFKRLINKDLGQQLAHLVLVNNPPQHPKGDFAFCKDQSNNLVLPEGEE
                     KYTFAGVSVLSPQLFADLPAGKAPLAPLLRQAIAQGLVSAEHHAGAWVDVGTPERLAD
                     LDQLILSQHI"
     gene            1489..>2144
                     /gene="rpe"
                     /locus_tag="PLY05_07055"
     CDS             1489..>2144
                     /gene="rpe"
                     /locus_tag="PLY05_07055"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNC03100.1"
                     /translation="MKDFVIAPSILSADFARLGAEVDAVLAAGADWVHFDVMDNHYVP
                     NLTVGPMVCKALRKYGVTAPIDVHLMVSPVDRIIGDFIEAGASYITFHPEASDHIDRS
                     LQLIKSGGCKAGLVFNPATPLHYLDYVLDKVDMVLLMSVNPGFGGQQFIAKTIDKIAQ
                     ARQIIDNSGFDIRLEVDGGVTTKNIRQIAQAGADSFVAGSAIFGAESYQQAINTMRQE
                     "
     assembly_gap    2145..2244
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2274..3758
                     /gene="trpE"
                     /locus_tag="PLY05_07060"
     CDS             2274..3758
                     /gene="trpE"
                     /locus_tag="PLY05_07060"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNC03101.1"
                     /translation="MLSLEQFSAFATQGYNRIPLALQLLADFDTPLSVYAKLANQPYT
                     YLFESVHGGERWGRYSMIGLAAQKRLEVRGHTVQLYQQQQLIDEQTLDDPLAFIEQYQ
                     QQFKVPVVEGLPRFTGGLVGYFAYDTVRLFEKKLAKIQKPDPLNVPDIQLMLSEEVVV
                     FDNLKESVFIIVHVDPQQPDAYQQGVQRLQQLALQLQQNNVCLPTYVETSTPISEADF
                     VSSFGQDAFKAGVERVKEYILAGDVMQVVPSQRMTVTFKHSPLALYRALRHLNPSPYM
                     YFVNCGDSHIVGSSPEILARVQEGKVTVRPIAGTRPRGKTPELDNALAQELLSDPKEI
                     AEHLMLIDLGRNDAGRVSEVGKVQVTEQMVIERYSHVMHIVSNVEGVLKPNVSAIEVL
                     KAALPAGTLSGAPKVRAMEIIDELEPIKRGIYGGAVGYLGWNGEMDTAIAIRTAVLKD
                     GVMHAQAGAGVVADSIPESEWQETLNKLRALFKAAELASKGLKA"
     gene            3892..3967
                     /locus_tag="PLY05_07065"
     tRNA            3892..3967
                     /locus_tag="PLY05_07065"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3925..3927,aa:Thr,seq:tgt)
     gene            4028..4112
                     /locus_tag="PLY05_07070"
     tRNA            4028..4112
                     /locus_tag="PLY05_07070"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4062..4064,aa:Tyr,seq:gta)
     gene            4133..4206
                     /locus_tag="PLY05_07075"
     tRNA            4133..4206
                     /locus_tag="PLY05_07075"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4165..4167,aa:Gly,seq:tcc)
BASE COUNT         1191 a          803 c          936 g         1253 t
ORIGIN      
        1 gattttgagc agggctttat gttgttaagt gatttgggcg ataccttatt aagaccgtgt
       61 ttaaccgaac aaaccgttga tggtttatat caacaagcga tgcgtgaaat tttagtcatg
      121 cagcaaagtc catcacctga aaactatccc attccacctt acgatcaaac gcgcttattg
      181 tttgaaatga gtttgtttaa agattggttt gtggcgcaat atttggactt atcattaaca
      241 cctgaagaaa tcaaagtgat tgacgactgt tgtcaacaaa ttgccaccgc cattgctgag
      301 caaccgacag tgtttgtcca tcgtgattat cactcacgta atttaatgtt attggctaat
      361 ggcaaattag gtgtgattga ctttcaagat gcactcatgg gcgcaattac ttatgacctc
      421 gtctctttat tgcgagatgc ttatgtcgaa tggccaaatc agcaagttgt tgcatgggtt
      481 gaagatttta gacaattatt agttgcacaa gggcgattaa caacagtcag tcaaccacag
      541 tttttaacat ggtttgattg gatgggggca caacgtcatc ttaaagtgct agggattttt
      601 gcacgcttaa gtattcgtga tggcaaacat ggatatttaa acgatatacc tttggtgttt
      661 aaatatttat taaatgaaat caaagactac cctgaattta atgctttaaa ccagttatta
      721 attagcaaaa tcctgcccag ttatttagcc aaaaatccac aagcagcagc aatgttaggg
      781 gaatttgtac aatgaaagcg atgattttag ctgctggttt aggcaatcgt atgcgcccct
      841 taaccttaac aacaccaaag cctctattag ctgtgggagg taaaccctta attgtttggc
      901 atattgatgc cttaaaagcg gtaggtatta ccgaattagt gattaatacc gcatggttgg
      961 ggcataagct gcacgaagct ttaggcgatg gcgcggcgta tggtgtaaat attcagtggt
     1021 cagatgagca agagccttta gaaactgcag gtggcattca gcaagcatta cccttattgg
     1081 gtaatgagcc atttttgcta attaatggcg atatttggtt acgttacgat tttaaacgtt
     1141 taatcaataa agatttaggc caacagttgg cgcatttggt gttagtcaat aatcccccac
     1201 aacatcccaa aggcgatttt gcattttgca aagaccaatc aaataactta gttttgcctg
     1261 aaggtgaaga aaaatatact tttgcagggg tcagtgtatt atccccgcaa ttatttgccg
     1321 atttgcccgc aggtaaagcc ccattagccc ctttgttacg tcaggccatt gctcaaggtt
     1381 tagtctccgc agagcatcat gcaggggcat gggtggatgt ggggacaccc gaacgtttag
     1441 cggatttaga tcaattaatt ctttctcaac atatttagcg agtttcacat gaaagatttc
     1501 gtcattgctc cttcgatttt atctgctgat tttgcccgat taggcgcaga agtagatgcc
     1561 gttttagctg caggagcaga ctgggtacat tttgatgtga tggataacca ttatgttccc
     1621 aacctcactg ttgggccaat ggtctgtaaa gctttacgca aatatggcgt cacagcaccg
     1681 attgatgtgc atttaatggt cagtcctgtc gatagaatta ttggtgattt tattgaagca
     1741 ggagcaagtt atattacctt ccatccagaa gcctcagatc atattgatcg ctctttacag
     1801 ttaattaaaa gcggtggttg taaggcgggt ttagtattta atcctgcaac acccttgcat
     1861 tatttggatt atgtgttaga taaagtagat atggtgttgt taatgagtgt caaccctggt
     1921 tttggtggtc aacaatttat tgccaaaacc attgataaaa ttgcgcaagc tcgacaaatt
     1981 attgataaca gtggttttga tattcgttta gaagtggatg gtggcgtcac aaccaaaaat
     2041 atccgtcaaa ttgcccaagc aggagcagat agctttgtgg caggttcggc aatttttggt
     2101 gcagaaagtt atcagcaagc aatcaacacc atgcgtcagg agttnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnntaaatt agccaaaata ttagggtgtt tttatgttat
     2281 ctcttgagca attctctgcc tttgccacgc aaggctataa tcgtattcct ttagcattac
     2341 aacttttagc cgattttgat accccattat cggtctatgc caaattggcc aatcagccat
     2401 atacctattt atttgagtct gtacatggtg gtgaaagatg ggggcgttat tccatgattg
     2461 gtttagcggc acaaaaacgt cttgaggttc gtggtcatac tgtacaactc tatcaacagc
     2521 aacaattgat agatgaacaa accttagatg atcctttagc cttcatcgaa caatatcagc
     2581 aacaatttaa agtgcctgtt gttgaaggct tgccacgttt tacaggtggt ttggtgggct
     2641 attttgctta cgatactgtc cgtttgtttg aaaagaaact tgctaaaatt caaaagccag
     2701 acccgttgaa tgtgcctgat attcagttga tgttgtcaga agaagtggtc gtttttgata
     2761 acctaaaaga aagtgtcttt ataattgttc atgttgatcc acaacaaccc gatgcttatc
     2821 aacaaggtgt acaacgttta caacaacttg ctttgcagct gcaacaaaat aatgtttgtt
     2881 tgccaacata tgtagaaaca tcaaccccca tcagtgaggc ggattttgtt tctagttttg
     2941 gtcaagatgc atttaaagca ggcgttgagc gcgttaagga atacattttg gcgggtgatg
     3001 tgatgcaagt tgtgccatca caacgcatga ccgtcacctt taagcattcg ccattagcct
     3061 tatatcgtgc tttgcgtcat cttaatccct caccttatat gtattttgtc aattgtggtg
     3121 atagccatat tgtcggttct agccctgaaa ttttggcacg tgtacaagag ggcaaagtca
     3181 cagtacgacc aattgcaggc actcgtccac gtggtaaaac acctgagctc gataatgcct
     3241 tggctcaaga gttgttatct gatcccaaag aaattgctga acatttgatg ttgattgatt
     3301 taggacgtaa tgatgcaggt cgagtcagtg aagtgggtaa agtccaagtc acagagcaaa
     3361 tggtcattga gcgttattct catgtcatgc acattgtttc caatgttgaa ggtgtactca
     3421 aacctaatgt cagtgcgatt gaggtattaa aagccgcttt acctgcagga actttgtcag
     3481 gtgcacctaa agtacgtgcc atggaaatta ttgatgagct agagccaatt aagcgcggta
     3541 tatatggcgg ggcagtgggc taccttggct ggaatggaga aatggatact gccattgcca
     3601 ttcgtacggc tgtactaaaa gatggtgtga tgcatgcgca ggctggggca ggggtagtag
     3661 cggattctat tcctgagtca gaatggcaag aaactttaaa taagcttcgt gccttattta
     3721 aagcagcaga attagccagt aaagggctaa aagcgtaatc ttgtccaaaa aacacgcgat
     3781 atgattaaaa aaagtgcatt cgatgcaaaa agctgcaaaa acttaaaaaa aaatgcaata
     3841 agtacttgcg ctactaaaaa aaattgctag aatgcacccc actttcgcaa tgccggtata
     3901 gctcagttgg tagagcaact gacttgtaat cagtaggtcg tgagttcgag tcccactgcc
     3961 ggcaccattc ttaaagcgat aaatctagag cttacctgac cataggctca aattgcagta
     4021 ccgcgttgga gaggttgggg agcggtcaaa cccatcagac tgtaaatctg ccgtcttaga
     4081 cttcgaaggt tcgaatcctt ccctctccac catatttacc aaaaaataaa gcgcgggtgt
     4141 agtttagtgg tagaacttca gccttccaag ctgatagtgc gggttcgatt cccgccaccc
     4201 gctccaatta ggtaagcttt attgttaggc tcatatagct cagtcggtag agcacttcct
     4261 tggtaaggaa gaggtcaccg gtt
//
LOCUS       DAOQMB010000186         4282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10034_length_4282_cov_13.481665, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000186 DAOQMB010000000
VERSION     DAOQMB010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10034_length_4282_cov_13.481665"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLY05_07080"
     CDS             complement(<1..1073)
                     /locus_tag="PLY05_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03102.1"
                     /translation="MKNIPFAPKILSAAVLSVLLAACGGGGDDDGTATVAPVVTESSI
                     TGTAVKGLLQAAKVTAYKVVDGKKGDKITETTTDNNGAYTLKVSGYSGAVILEMTADA
                     STKMLCDIPTGCEGSVEFNAPIGVNGLVLQTVLPELKATNKTAITPFTHLAAKYAEDK
                     GFNKTNIETALTQIADLFNLPALNDTTAVNPSGNLATANTAEQQYAIMNAAIAQLAGK
                     VADISAKLNALAVEINSKNGQLQSSEAVGKNDKLDLADVLAAAKAVAESNQFKNAVNS
                     AVKTLLAAQLELANKSQDITTAAPTNGAGLDDLAKAKAFVNSASLLLSTLQQYDDQSF
                     IKTAEQKVKSIQALTDGDSLIFDA"
     gene            complement(1219..1542)
                     /locus_tag="PLY05_07085"
     CDS             complement(1219..1542)
                     /locus_tag="PLY05_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC03103.1"
                     /translation="MAFVVTENCIKCKYQDCVEVCPVDCFYEGPNFLVIHPDECIDCA
                     LCEPECPANAIFSEDEVPKGQEQYIKLNAELAEVWPNITEKGDAPADAKQWDGVKDKL
                     QYLER"
     gene            complement(1674..4256)
                     /gene="mutS"
                     /locus_tag="PLY05_07090"
     CDS             complement(1674..4256)
                     /gene="mutS"
                     /locus_tag="PLY05_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNC03104.1"
                     /translation="MMQQYLRIKADYPHALVFYRMGDFYELFFDDAHKAARLLDITLT
                     HRGKTAGNPIPMAGVPFHAAEGYVAKLVKLGEAVVICEQVGDPATSKGPVERKVVRIL
                     TAGTVTDEAFLDAKQEALLLAIHAYQQELGLAVLDISSGRFSVLSCELSPDGLAAELA
                     RLNPAEILISEDSPVLAWLDNRPIRKYPPWQFGLETAQHILCQQFAVKDLQGFGCQHL
                     PAAIIAAGALLQYAKETQKTALPHLQGLRVEQASDFIYLDAATRKNLELTQTLVGEFQ
                     YSLAWVLDVCQTPMGSRLLRRWLHQPVRHVPTLLARQAAITELSTDYYYEKLQSHLQA
                     IGDCERILARVALKTARPRDLSRLREVFAELPALQQQLKLAKDSKIQALADDIQEFPQ
                     LHQLLSKAIVDNPPVVIRDGGVLAEGFDAELDELRAISTNAGDYLLQLELKEREATGI
                     ANLKIGYNRVSGYYIELTRAQADNAPAHFIRRQTLKNAERFITPELKVFEDKALSAAA
                     KALAREKFLYEQLLEGFSQYLAPLQHMSLALAELDVLVALADRAASLNWVKPQLVSQV
                     TVDIKQGRHPVVEAVLNSPFTANDTLLTSKQKMLIITGPNMGGKSTFMRQTALIVLLA
                     HIGSFVPAQAATIGCIDRIFTRIGSSDDLAGGRSTFMVEMTETANILQNATEQSLVLM
                     DEVGRGTSTFDGLALAWAAAVHLAQQIKALTLFATHYFELTSLPQYYQGVANVHVAAS
                     DYGDHLVFLHQVTEGAASKSFGLQVAKLAGVPMAVIQAAQHKLQELEQSPNAQKAKQK
                     PAPMQAGLFAEPVVVEKVVEKIIEKPQVSQVEQALKAIDIDNLTPRAALQQLYDLQAL
                     LMKS"
BASE COUNT         1241 a          943 c          819 g         1279 t
ORIGIN      
        1 gcatcaaaaa tcaaactatc accgtcagtt aatgcttgaa tggacttaac tttctgctct
       61 gcggttttaa taaagctttg gtcatcatac tgttgtaagg tacttaataa caaactagca
      121 ctattaacaa aggctttagc tttagccaaa tcatctaaac cagccccatt agtaggagct
      181 gcggttgtta tatcttggct tttgttagct aactctaatt gtgctgccaa tagagtttta
      241 actgcgctat ttacagcatt tttaaactga ttggactcag caacggcttt tgcagctgcc
      301 aatacatcag caaggtctaa cttgtcattt ttaccaacag cttcgctgct ttgtaactgg
      361 ccatttttgc tattaatttc cacagccagt gcgtttaatt tagccgaaat atccgccact
      421 ttgcctgcta actgagcaat cgccgcattc ataatcgcat attgttgctc tgcggtatta
      481 gctgttgcta aattacctga tggattaacg gctgttgtgt catttaatgc aggcaaatta
      541 aataaatcag caatttgtgt taaagctgtt tcaatatttg ttttattaaa gcctttgtcc
      601 tcggcatatt tggccgctaa atgggtaaag ggcgtaatgg ctgttttgtt ggtagctttt
      661 aactctggca atacggtttg taaaaccaaa ccattcaccc caataggtgc attaaactcg
      721 acactgccct cacaacctgt tggaatatca cacaacattt tagttgaagc atcggcggtc
      781 atttctaaaa taactgcacc actatagcct gatactttaa gtgtatatgc accgttattg
      841 tcggtggttg tttcggtgat tttatcacct ttttttccat caactacttt ataagcggtg
      901 actttagccg cttgtaataa acctttaacg gctgtacctg taatacttga ttctgtaaca
      961 acaggagcga ctgttgctgt tccatcatcg tcaccaccgc caccacaggc agcaagtaat
     1021 actgataaaa cagcagcaga aagaatttta ggtgcaaatg gaatgttttt cattagaaac
     1081 ttccttgatt aaataaaacg tgattcgagt gcaaaatact atcattgaaa aacaattcaa
     1141 aaaactggct agccaacaaa aacattacaa aacgtaacaa aaaagccgcg aaattcgcgg
     1201 cttttttgtt aaaacaaatt aacgctctaa gtattgtaat ttgtctttta caccatccca
     1261 ctgtttagcg tcagcaggtg catcaccttt ttcggtgatg tttggccata cttcggctaa
     1321 ttcagcattg agcttgatgt attgttcttg accttttggc acttcatctt cagagaaaat
     1381 cgcatttgct ggacactctg gctcacacaa agcacagtcg atacattcat caggatgaat
     1441 aaccaaaaag tttggacctt catagaagca atctacagga catacttcta cgcaatcttg
     1501 atatttacat ttaatacagt tttcggtaac gacaaaagcc atgttgtcct gcctcttaat
     1561 ttaaacaatc aaatttaagc tgatataacg cctaaatctt aaccaatggg aacataattc
     1621 aacaatagtc ttattctacg catgattaat cccatttgta agccctaatc tacctagctt
     1681 ttcatcaata atgcttgtaa gtcatataac tgttgtagag cagcacgagg cgttaggttg
     1741 tcaatatcta tcgcttttag ggcttgttcg acttggctta cctgtggctt ttcaataatt
     1801 ttttcgacca ctttctcaac aacaacaggt tcggcaaata agcccgcttg catgggagca
     1861 ggtttttgtt tggctttttg agcattagga ctttgttcta gctcttgtag tttgtgctga
     1921 gcggcttgaa taaccgccat aggcacacct gctaacttag cgacttgcaa accaaaactt
     1981 ttactggctg cgccttcggt gacttgatgc aaaaatacta aatgatcgcc ataatcactc
     2041 gcagcaacat ggacattcgc gacgccctga taatattgtg gcaaactggt aagttcaaaa
     2101 taatgggttg caaacaaagt taacgctttg atttgttgcg ccaaatgcac agccgctgcc
     2161 catgctaagg ctaaaccatc aaaggtgctt gtacctctcc ctacttcatc cattaacact
     2221 aaactttgtt cggtagcgtt ttgtagaata ttcgcggttt cggtcatttc aaccataaag
     2281 gttgaacggc cacctgctaa atcatcagat gagccaatac gggtaaaaat tcgatcgata
     2341 caaccaatag tcgctgcttg tgcaggcaca aaactgccaa tatgcgctaa tagcacaatc
     2401 aacgccgttt gacgcataaa cgttgattta ccgcccatat ttggccctgt gataatcaac
     2461 attttttgtt tagaggttaa taaagtgtcg tttgcggtaa atgggctatt taacacggct
     2521 tcaaccacag gatgacgacc ttgtttaata tcaacagtaa cttgtgacac cagttgtggc
     2581 ttcacccaat ttaacgaagc tgctctatcg gccaaagcca ccaacacatc aagctctgcc
     2641 aaagctaaac tcatgtgttg tagcggagct aaatattgtg aaaaaccctc taaaagttgc
     2701 tcgtataaga acttttcacg agctaaggct tttgcggcag cagataaggc tttgtcctca
     2761 aacactttga gttcgggcgt gataaaccgt tcggcatttt ttagggtttg acgacgaata
     2821 aaatgggctg gggcgttatc ggcttgggca cgagttagct caatataata accactgaca
     2881 cggttatagc caatttttaa gttggcaata cctgttgctt cgcgttcttt taattctagt
     2941 tgtaataaat aatcgcctgc attggtgcta attgcgcgta attcatctaa ctctgcatca
     3001 aaaccttctg ccaatacgcc accatcacga atcaccacag gtgggttgtc aacaatggct
     3061 ttgctcaaca actgatgtaa ttgtggaaac tcttggatat catctgctaa ggcttgaatt
     3121 ttgctgtctt tagccagttt taattgctgt tgcaaggcgg gcaattcggc aaacacttct
     3181 cgcaaacggc ttaaatcacg cggacgtgcg gtttttaatg ccacccttgc taaaattcgc
     3241 tcacaatcac caattgcttg caaatgactc tgcaattttt cgtaataata atcggtgctt
     3301 aattcggtaa tagctgcttg tcgagctaac aatgtcggca catgacgaac aggctgatgt
     3361 aaccaacgac gtaataaacg gctacccatc ggtgtttggc aaacgtctaa cacccatgcc
     3421 aaactatatt gaaactcacc cactaaggtt tgcgttaact ctagattttt gcgagtggct
     3481 gcatctaaat aaataaaatc actggcttgt tcgacgcgta aaccttgtag atgtggtaaa
     3541 gcagtttttt gggtttcttt ggcatattgc aacaatgcac cagcggcaat aatggctgca
     3601 ggtaaatgtt gacaaccaaa accttgcaaa tctttaacgg cgaattgttg gcataagata
     3661 tgttgtgccg tttctaaacc aaattgccac ggcggatatt tacgaattgg ccgattatca
     3721 agccatgcta atacaggcga atcctcacta attagaattt cggcaggatt taaacgagct
     3781 aattcagcag ctaaaccatc aggcgataac tcacaactga gtacactaaa acgaccacta
     3841 ctaatatcta aaaccgctaa acctaattct tgctgatagg catgaatcgc taataataaa
     3901 gcctcttgtt tagcatctaa aaatgcttca tcagtcactg tacctgcggt taagatacgg
     3961 acaaccttgc gttcaacagg ccctttgctg gtggcaggat cgcctacttg ttcacaaatg
     4021 accaccgcct cgccaagctt aaccaacttt gccacataac cttcggccgc atgaaatggc
     4081 acacctgcca tgggaatggg atttcctgcg gttttgccac gatgggttaa ggtaatatct
     4141 aataaacgtg cagccttatg cgcatcatca aaaaacaatt cataaaaatc gcccattcga
     4201 taaaaaacta aggcatgggg ataatcggct ttgatgcgta aatattgctg catcatcggg
     4261 gtgtggtcgg caaaatcagt ca
//
LOCUS       DAOQMB010000187         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10095_length_4267_cov_14.264482, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000187 DAOQMB010000000
VERSION     DAOQMB010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10095_length_4267_cov_14.264482"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..265)
                     /locus_tag="PLY05_07095"
     CDS             complement(26..265)
                     /locus_tag="PLY05_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03105.1"
                     /translation="MSRSKRHTPKCGICKCDSEKSDKKIWHQRMRAAIKQYLHATNFD
                     EKLPPHVKEVSNVWAFGKDGKSWFNQKNHPDLMRK"
     gene            complement(351..1244)
                     /locus_tag="PLY05_07100"
     CDS             complement(351..1244)
                     /locus_tag="PLY05_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC03106.1"
                     /translation="MSNKKRGLGNGRGLDALLGAVRQAKEEVAAVETGHIPQVGMSLL
                     NLPLEQLQRGRYQPRRDMNPVALQELADSIKAQGVMQPIVVRSIGENSYEIVAGERRW
                     RAAQMAGLDSIPAIIRQINDDTAMAMALIENIQREDLNPMEQATAMHRFVEEFGLTHQ
                     QIAEAVGKARATVSNLLRLMALPREVKTLLEHGDIEMGHARALLALPENQQAEAARLT
                     VAKGFSVRQTESLVRSLLAAQDAPQPEKRVDPNVAQLQQSLIDKLGAAVQIDYNKKGQ
                     GKLIIRYNSFEELDGILAHIQ"
     assembly_gap    1298..1397
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1398..1780)
                     /locus_tag="PLY05_07105"
     CDS             complement(<1398..1780)
                     /locus_tag="PLY05_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013198089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03107.1"
                     /translation="MTQILAIANQKGGVGKTTTTVNLAASLAAVKRRVLLIDLDPQGN
                     ATMGSGVDKNNLPYSVYDVVLGDTPIKVAIQKAPKAGYDVLGANRDLSGAEISLFDLE
                     RRELRLKEAIEQIAKSYDFILIDCAP"
     gene            complement(1802..2425)
                     /gene="rsmG"
                     /locus_tag="PLY05_07110"
     CDS             complement(1802..2425)
                     /gene="rsmG"
                     /locus_tag="PLY05_07110"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNC03108.1"
                     /translation="MSTPQQLLNQGIDQLGLNIAPQAITQLEFYMAALQKWNAAFNLT
                     AIREPKDIVIKHFLDSLTVLPHVKHDTLLDVGTGAGFPALVLAIAQPQLQVDLLDSNS
                     KKTRFLRQMVAELALKNVRVHHARVEQANLTAQKQVISRAFSSLVDFCQWCEHLLAED
                     GVLLAMKGQYPQAEIDDMPASMRVLSSTPLNVPFLDEARHLLVLGRT"
     assembly_gap    2564..2663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2664..4255)
                     /gene="mnmG"
                     /locus_tag="PLY05_07115"
     CDS             complement(<2664..4255)
                     /gene="mnmG"
                     /locus_tag="PLY05_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNC03109.1"
                     /translation="MRYPKVYDVIVIGGGHAGTEAALAAARMGCQTLLLTHNVETLGQ
                     MSCNPAIGGIGKSHLVKEIDALGGAMALATDKGGIQFRVLNGSKGPAVRATRAQADRV
                     LYKAAVRSILENQANLDIFQQAADDLIVEGDTVKGVISQTGIHFSATSVVLTTGTFLG
                     GTIHIGLENYRGGRAGDPPSIALAHRLRELPLRVGRLKTGTPPRIDAKSVDFSVMTEQ
                     PGDTPTPVMSYLGSLEMHPKQISCWITHTNEKTHDIIRSGLDRSPMYTGVIEGVGPRY
                     CPSIEDKIHRFADKLSHQIFIEPEGLNTHELYPNGISTSLPFDVQIQLVRSMKGLENA
                     HIIRPGYAIEYDYFNPQDLQHTLETKVIKNLFFAGQINGTTGYEEAGAQGLLAGINAG
                     LRAQDKEGWYPTRDQAYLGVLVDDLITLGTKEPYRMFTSRAEYRLLLREDNADARLTE
                     IGRKLGLVDDVRWRVFSEKQEAIAQQTQRMKETFIHPQTELAQRFTEMTGQELARENN
                     LLDLLKRPQVSFEQLATLTETQV"
BASE COUNT         1116 a          961 c          800 g         1190 t
ORIGIN      
        1 ttcgttcctc aacccatgct acagactact ttctcattaa atcaggatga tttttctgat
       61 taaaccaact cttaccatct ttaccaaatg cccacacatt agaaacttct ttaacatgtg
      121 ggggtaattt ttcgtcaaag ttagtcgcat gtaaatattg tttaattgcc gcacgcatac
      181 gttgatgcca tattttttta tcggactttt cactgtcaca cttacatatt ccacatttag
      241 gcgtatgacg tttggagcga ctcattttta taatttccca aatatgtaat gtgggttgac
      301 gcaggaaacc ctcaaataac tatatcatgg gtttcgttcc tcaacccatg ctactgaata
      361 tgcgccaaaa taccatccaa ctcttcaaac gagttataac gaataattag cttaccttgg
      421 ccttttttgt tgtaatcaat ctgcactgct gcacccaact tatcaatcaa gctttgctgt
      481 aattgtgcaa cattggggtc aacacgcttt tcgggttgtg gtgcatcttg tgcagctaac
      541 aaactacgca ctaatgactc ggtttggcgc accgaaaagc ctttagcaac ggttaatctt
      601 gccgcttctg cctgctgatt ttcgggtaac gccaacaaag cacgcgcatg ccccatttca
      661 atatcgccat gctctaataa cgtttttact tcgcgcggta aggccattaa acgcaataaa
      721 ttactgactg tggcgcgggc tttacccacc gcttcagcga tttgttgatg ggttaaacca
      781 aactcttcaa caaaacggtg cattgccgtt gcttgctcca ttgggtttaa atcttcacgt
      841 tgaatatttt caatcaacgc catcgccatt gccgtatcat cattaatttg acgaataatc
      901 gcaggaatac tgtccaaacc tgccatttgt gccgcacgcc aacgacgctc acctgcgaca
      961 atttcatagc tattttcgcc aatacttcta actacaatcg gctgcatcac accctgtgct
     1021 ttaatagaat cggctaattc ttgcaaagca acaggattca tgtcgcgacg tggttgataa
     1081 cgaccacgct gcaattgctc taagggtaaa tttaataaac tcatacccac ttgcggaata
     1141 tgacctgttt caacggcagc cacttcttct tttgcctgac gaacagcacc taacaaggca
     1201 tctaaaccac ggccattgcc taaaccacgt tttttgttac tcatgctgtt tttctctttt
     1261 tatctttttt aatcaattcg gcagccaaac ttaaatannn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnggc gcacagtcga ttaaaataaa atcataactt ttggcgattt
     1441 gttcaatggc ttcttttagg cgcaattcac ggcgttctaa atcaaataag ctaatttctg
     1501 caccgcttaa atcccgattc gcacccaaaa cgtcataacc tgcttttggc gctttttgaa
     1561 tggcgacttt aatgggggta tcccccaaaa ccacatcata aaccgaataa ggcaaattat
     1621 ttttatcaac tcccgatccc atcgtggcat taccttgtgg gtctaagtcg attaataaca
     1681 cgcgacgttt tactgctgct aaagaagcgg ctaagttaac ggtggttgtg gttttgccaa
     1741 caccaccttt ttgattagca atcgccaaaa tttgcgtcat tttgtgactt cctaataaaa
     1801 cttatgttcg tcccaatacc aataaatgtc gggcttcatc caaaaaaggc acattcaagg
     1861 gcgtcgatga taacacgcgc atgcttgctg gcatgtcatc aatttctgct tgtggatatt
     1921 gtcctttcat cgccaacaat acgccatcct cagccaataa atgttcacac cattgacaaa
     1981 aatccaccaa actagaaaac gctcggctaa taacttgttt ttgtgccgtt aaatttgctt
     2041 gctcaacccg tgcatgatgc acacggacat tcttgagtgc taattctgcc accatttgcc
     2101 gtaaaaaacg ggtctttttg ctattactgt ctaacaaatc gacttgtaat tgtggttggg
     2161 caatcgccaa caccaacgca ggaaaccctg cgcctgtacc cacatctaac aatgtgtcat
     2221 gtttaacatg gggtaacacg gttaatgagt ccaaaaagtg tttaatcaca atatctttgg
     2281 gctcacggat ggcagtgaga ttaaaagcgg cattccattt ttgcaaagca gccatataaa
     2341 actcaagttg agtaatggct tggggtgcaa tatttaagcc gagttggtca ataccttgat
     2401 tgagcagttg ttgaggcgta gacataatag gataaaattt ttagacaggt ataagatcag
     2461 gggcaaaata tctttgcccc tcagtcgaca gtaaaagcgt ttagcccacc aatttcatat
     2521 tggctaaacc gctctttttc aaataaatca tcaacaatga cacnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnacttgtg tctcagtcaa agtggccaat tgttcaaagc
     2701 tcacttgtgg gcgttttaac aagtctaaca aattgttttc acgcgctaat tcttggccag
     2761 tcatttcggt aaagcgttgc gccaattcgg tttgtggatg aataaaggtt tctttcatgc
     2821 gttgggtttg ttgggcaatg gcttcttgct tttcgctaaa tacacgccaa cgcacatcat
     2881 ccactaaacc tagtttgcga ccaatttcgg ttaaacgcgc atcggcattg tcttcacgca
     2941 acaacaaacg atattcggcg cgtgaggtaa acatgcggta tggttctttt gtacccaacg
     3001 taatcaagtc atcaaccaat acccctaaat aggcttggtc acgggttgga taccagccct
     3061 ctttgtcttg agcgcgtaaa ccagcgttaa tgcctgctaa taaaccttgt gcacctgctt
     3121 cttcataacc cgttgtgccg ttaatttgcc ctgcaaagaa taagttttta atcactttgg
     3181 tttcgagggt atgttgcaaa tcttgtggat taaaataatc gtactcaatg gcataacctg
     3241 gtcgaataat atgggcgttt tctaaaccct tcattgaacg aaccaattga atttgcacat
     3301 caaacggcaa actggtggaa ataccattcg gatacaattc gtgagtattt aaaccttcgg
     3361 gctcgataaa aatttggtgt gataatttgt cggcaaaacg atgaattttg tcttcaatag
     3421 acgggcaata acgcgggcca acaccctcaa ttacgcctgt gtacatcggt gaacgatcta
     3481 aaccactgcg aataatgtca tgggtttttt cgttggtatg ggtaatccag caactaattt
     3541 gtttggggtg catctccaaa ctgcccaaat aggacatgac aggcgtgggt gtgtcacctg
     3601 gttgttcggt catcacgcta aaatcaacac ttttggcatc aatacgcggt ggtgtaccag
     3661 tttttaaacg accaacacgt aatggcaatt cacgcaaacg atgtgctaaa gcaattgaag
     3721 gtggatcacc tgcacgacca ccacggtagt tttctaagcc aatgtgaatc gtaccaccca
     3781 aaaacgtacc tgtggttaac accacagagg tggcagaaaa gtggatacct gtttggctaa
     3841 tcacgccttt aacagtatcg ccttcgacaa tcaagtcatc agccgcttgt tggaaaatat
     3901 cgaggttagc ttggttttcg agaatgctac gcacagccgc tttgtaaagt acacggtcgg
     3961 cttgggcgcg tgtggcgcgg acggctggcc ctttgctgcc atttaatacc cgaaattgga
     4021 tgccaccttt gtcggtggct aaggccattg ccccacctaa agcgtcaatt tctttgacta
     4081 aatggctttt gccaatgccg ccaatggctg ggttacagct catttgacct aaagtttcga
     4141 cgttatgcgt caacaatagg gtttgacagc ccatacgagc agcggctaag gctgcttctg
     4201 tgcctgcatg gccaccacca atgacaatca catcgtaaac tttaggataa cgcataacac
     4261 cacaccg
//
LOCUS       DAOQMB010000188         4257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10135_length_4257_cov_17.757972, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000188 DAOQMB010000000
VERSION     DAOQMB010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10135_length_4257_cov_17.757972"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..727
                     /locus_tag="PLY05_07120"
     CDS             44..727
                     /locus_tag="PLY05_07120"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNC03110.1"
                     /translation="MERQRVSVQEATKQRETSIALFESNLKELKAYAPTPEMNEGLKK
                     VETVWMGYKKRVLQTPSREEALTVIRQSDEVLKLSESVVKMVQEYSKVSSARLVNISG
                     RQRMLSQRIAKLYLTKSWGLTYEGLDQDLTTAINDYENALAELTASPLNTDEIKQGLG
                     KVQGIWKFSKSGFALSNENLYVPTAIANTTENLLKHMQAITKQYEVQMQIAANKSSNN
                     SNNNSSNKT"
     gene            complement(755..1168)
                     /locus_tag="PLY05_07125"
     CDS             complement(755..1168)
                     /locus_tag="PLY05_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03111.1"
                     /translation="MLVVKRWLQFVAIVHIIMGLLLPIIVHIDLVQPYFRLMADTFAL
                     NHQQDLLFLQFMIGLFGPTVASWGVLFFGIVTYAFSQPNKGVWWLMILACVVWAAYDS
                     IYSSLFGLWINAVINAIAFVSIVLPLCMVKKEFCD"
     gene            1292..1594
                     /locus_tag="PLY05_07130"
     CDS             1292..1594
                     /locus_tag="PLY05_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03112.1"
                     /translation="MTTIKQIRMTQIASLIGLVIIIIIGLTFVGAILGLYHLPTEKGN
                     FLGEIFLVDSCITGSLLLIVRRARCPVCKNVFVGKTEPRWFSKTCKNCGRRSGDTH"
     gene            complement(1718..2464)
                     /locus_tag="PLY05_07135"
     CDS             complement(1718..2464)
                     /locus_tag="PLY05_07135"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNC03113.1"
                     /translation="MKRLVLAAALSMTTIAWAAEPVAATPATTVFANEDAKVAYSLGY
                     IFGKNNATAIENLDIEKFVAGFKEGYAGKQGALSEEEIKKILTDYKERRVAEAQAEFQ
                     KQSADNKARSETFLAENAKKTGVVTTTSGLQYEVLTQGTGIQPKATDTVEVHYEGKLT
                     DGTIFDSSIQRGQTASFRLDQVIAGWTEGLQLMKAGSKYRFTIPSALAYGEMGAGSIP
                     PNAVLVFDVELIKVTKEEGAAKTEKKAKKK"
     gene            2866..3555
                     /locus_tag="PLY05_07140"
     CDS             2866..3555
                     /locus_tag="PLY05_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03114.1"
                     /translation="MPSKKKAAKTEQLVVDTSEQLQKLAKKAQKEAEKYIESARKLVE
                     KAEASYKDILAKLAQVIKNGDVEAKKLLEDAQSHAEAALEAAKKDYEKAKSIFQTSEL
                     RSYVQTKLAKGKEAAVALVEEVKKEATQAVAAVEKEAANLAQEVKKDAAQVAEAVEKA
                     TAPVKRAAARKAPAKKATTEAATPAAPKRPATRKAPVKKAPVADATPATTDAPAAEAA
                     ATTSSNETPAA"
     gene            complement(3607..3924)
                     /locus_tag="PLY05_07145"
     CDS             complement(3607..3924)
                     /locus_tag="PLY05_07145"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HNC03115.1"
                     /translation="MSGILMCNELDQAKKWVFDHIQQYGKIAALIVIEPELQGLQELV
                     EWQDIEHDEYIQQHTKALAIVGSEQWSEDAILFLLGGLLPFPIKYFQPKEQPLAEAWL
                     LSH"
     gene            4067..>4257
                     /locus_tag="PLY05_07150"
     CDS             4067..>4257
                     /locus_tag="PLY05_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03116.1"
                     /translation="MSYLSLIYRQNGIWLSKRHLSWQEWQIIYPDYISSLDNWSCEEL
                     TDFLQEEYPDLSPDAATQIA"
BASE COUNT         1438 a          851 c          720 g         1248 t
ORIGIN      
        1 caaagcctat ttgttctaca ataagtaggt tttgataagt tttatggaac ggcaacgagt
       61 aagcgtccaa gaagccacca aacaacgtga aacaagtatt gctctatttg aatctaattt
      121 aaaagagtta aaagcctatg cgcctacgcc tgaaatgaat gaaggtttaa aaaaggtcga
      181 aactgtttgg atgggctaca agaaacgtgt tttgcaaaca ccaagtcgcg aagaagcctt
      241 aactgttatt cgtcaaagtg atgaagtgtt aaaactcagt gaatcggttg tcaaaatggt
      301 tcaagaatac agcaaagttt ctagtgcgcg gttggtgaat atttcagggc gtcaacggat
      361 gctaagtcaa cgcattgcta aattatactt aaccaaaagt tggggcttaa cgtatgaagg
      421 tttagatcaa gacttaacca cggcaattaa tgattatgaa aatgccttag ccgaattaac
      481 tgctagcccg cttaatactg atgaaatcaa acaaggttta ggtaaagtgc aaggtatttg
      541 gaagttttca aagtcggggt ttgctttatc taacgaaaat ctttatgtgc caaccgcaat
      601 tgctaataca actgaaaact tactcaaaca tatgcaagcg attaccaaac aatatgaagt
      661 tcaaatgcaa attgctgcca ataaaagtag taataatagt aacaacaata gcagcaataa
      721 aacctaagtc atcactgaca taaacaagga tgttttaatc acaaaactct tttttaacca
      781 tacatagagg caacacaata ctcacaaaag caatggcgtt aattaccgca ttaatccaca
      841 aaccaaataa gcttgaataa atactatcgt aagcagccca aacaacacat gcgagaatca
      901 tcaaccacca aacacctttg ttgggttgac taaacgcata agtaactatg ccaaaaaaca
      961 aaacgcccca actcgccact gttggcccaa ataaaccaat cataaactgc aaaaacaata
     1021 aatcttgttg atgatttagc gcaaaagtat ccgccattaa ccgaaaatag ggttgcacca
     1081 aatctatatg aacaatgata ggcaataaca accccataat aatatgaact atggccacaa
     1141 actgcaacca gcgcttaaca actaacatac tgacacccta tcgtcatttc agctaaattt
     1201 atcggaaggc aatagccaat ttaatatata aaactataat aacaatgtga taactatcac
     1261 aaaaaatcta gcaatttcgt gagacgtatt agtgaccacc attaaacaaa ttcgtatgac
     1321 acaaatcgca agcctaattg gattagttat tatcattatc atcggattga catttgttgg
     1381 agcgattctt ggtctttatc atttacccac agaaaaaggt aattttttag gtgaaatttt
     1441 tttagttgat agttgtatta cagggagtct gttactgatt gtcagacgag cacgttgccc
     1501 tgtttgtaaa aatgtttttg ttggcaaaac cgaaccacgc tggtttagca aaacatgtaa
     1561 aaactgtggc cgtcgcagtg gtgatacgca ttaatatagg agcggatgaa gggagccgaa
     1621 gccttgccaa aagcgttata agcctctacc attaaattac aattgctaat tagagactgc
     1681 ttttacagtc tctaatcgta gactcagcga ttttatgtta ttttttcttc gcttttttct
     1741 ctgtttttgc tgcgccttct tctttagtga ctttgattaa ctctacatca aaaaccaaaa
     1801 ctgcatttgg tggaatagaa ccagcaccca tttcaccata agccaaagcg gaaggaatgg
     1861 taaaacgata tttactacca gccttcatta actgcaaacc ttcagtccaa ccagcaatca
     1921 cttgatctaa tctaaaagaa gctgtttgac cacgctgaat agaactatca aaaatggttc
     1981 catccgttaa tttaccttca taatggactt caacagtatc tgttgcttta ggttgaatac
     2041 cagtaccttg agttaacacc tcatactgca aaccagatgt tgtagtgaca acacccgttt
     2101 ttttggcatt ttctgctaaa aaagtctcgc tgcgagcttt gttatctgca ctttgttttt
     2161 gaaattctgc ctgtgcttcc gcaacacgac gctctttata atctgttaaa atctttttaa
     2221 tttcctcttc agataaagca ccttgcttac ctgcatatcc ttctttaaat cctgcaacaa
     2281 atttttcaat atccaaattc tcaatagcag tggcattatt tttgccaaaa atatacccta
     2341 aactataagc aacttttgca tcttcattag caaacaccgt tgttgcaggt gtcgcggcta
     2401 caggctcagc agcccacgcg atcgtcgtca ttgataatgc agctgccaaa actaaacgtt
     2461 tcatatatac tccaatctca gttgttttta tcatcttaga atttacgcgg ccatcgcctt
     2521 tagtgtacaa gacccgcttg ttttgacttt gttaaacaca agcattcggt aatatgcgca
     2581 aatctgactc tctaactaaa aaatattatt aagtcgctaa gcataagatg tgttatgttt
     2641 ttatgctaga caagccgcta ttatttttgg ctaatttact tagtaaaata gtaattttgg
     2701 tgttttaaac taaatttctt cttattatta tttaaaaaaa taaaaaaaca gtcatccaac
     2761 caataaattg tatttttgtt agacaatttt tattctatct gtcaccattt tgagttaatt
     2821 ttgctaataa gcccttacct cttttgattt tctaggagca ccaccatgcc aagcaagaaa
     2881 aaagccgcca aaactgaaca attagttgtt gatactagcg aacaattaca gaagttagcc
     2941 aaaaaagcgc aaaaagaagc tgaaaaatat attgaatctg ctcgcaaatt agttgaaaaa
     3001 gccgaagcta gctacaaaga cattttagct aaattggctc aagtcattaa aaacggcgat
     3061 gtagaagcta aaaaattgtt agaagacgct caaagtcatg ctgaagctgc tttagaagct
     3121 gcgaaaaaag actacgaaaa agctaaaagt attttccaaa ccagcgaatt acgttcttat
     3181 gttcaaacta aattagctaa aggcaaagaa gctgctgttg ctttagttga agaagtgaaa
     3241 aaagaagcca ctcaagctgt tgctgctgtc gaaaaagaag ctgctaatct tgctcaagaa
     3301 gtgaaaaaag atgctgctca agttgctgaa gctgttgaaa aagcaaccgc tcctgtaaaa
     3361 cgcgctgcag ctcgtaaagc tcctgctaaa aaagcaacta cagaagctgc aacacctgct
     3421 gcacctaaac gtcctgcaac acgcaaagct cctgttaaaa aagctccagt tgctgatgca
     3481 acaccagcta caacagacgc tcctgctgct gaagccgctg caaccactag cagcaacgaa
     3541 acaccagccg cctaagtatt tcgtggttga tgtccaaggg cgaccttagg tcgccctttt
     3601 tatttgttaa tggcttaata accaagcttc agctagaggc tgctctttag gttgaaagta
     3661 ctttattggg aatggtaaca aaccgcctaa taaaaataga atagcatctt ctgaccattg
     3721 ctctgaaccg acaatcgcta atgcttttgt atgttgttga atatattcat catgctctat
     3781 atcttgccat tcaactaact cttgtaaacc ttggagttct ggctcaatga caattaatgc
     3841 agcaattttg ccatattgtt gaatatggtc aaatacccat tttttggctt gatctagttc
     3901 attacacatt aatatgcctg acactttagc aatcgctaaa ttttgctcaa ataagcctaa
     3961 agcaatcgac ttaatttgat tccctaataa atataaaatt tgttttaatg ataatcatta
     4021 attacttgca atgctctaac aaattgtcta actaaagaat tattttatga gttacttatc
     4081 gctaatttat cggcaaaatg ggatatggct atccaaacgt catttatctt ggcaagaatg
     4141 gcaaataatc tatcctgact acattagctc attagataac tggtcatgtg aagagttaac
     4201 ggattttttg caagaagaat atccagattt atcgcctgat gccgctacac aaatagc
//
LOCUS       DAOQMB010000189         4246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10184_length_4246_cov_15.215700, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000189 DAOQMB010000000
VERSION     DAOQMB010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10184_length_4246_cov_15.215700"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLY05_07155"
     CDS             complement(<1..378)
                     /locus_tag="PLY05_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03117.1"
                     /translation="MQNKPIYSSIILLLCLSSTSLANDFSDNKRLYRYYNDKGIPTVS
                     DQVSEEHIRRGYDIVDRQMQVIRRIPPFDEAVYQKDKAKRDNAFKQQQEDAKILRLYS
                     SARDAELARNRQLDTLSTGIGYNS"
     gene            531..1301
                     /locus_tag="PLY05_07160"
     CDS             531..1301
                     /locus_tag="PLY05_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC03118.1"
                     /translation="METIFVPLIDSLILFVISLVANLLSALAGGGAGLLQLPALLHLG
                     LPFGMALATHKIASVALGVGATTRHWQTGKLKWRFALLILLAGLPGVWLGTQLILAIP
                     EQIAQFALGVLTLGLGVYSLLKKDLGLTSQRIHRDKIGLIIGCLGLFIIGILNGSLTS
                     GTGLFVTIWLVRWFGFDFTRAVSYTLILVGFFWNGLGALFLGLQTPVAWSWLPALLLG
                     SLIGGYLGAHWALLKGNQLVKRSFEVLTILVGLSLLFT"
     gene            complement(1290..3386)
                     /locus_tag="PLY05_07165"
     CDS             complement(1290..3386)
                     /locus_tag="PLY05_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC03119.1"
                     /translation="MTTLSPSITEALTWQISPLFVIFALICAGGHFYSITTISQHHHS
                     LFKDRSPLNLIWYLIINTVLALNIWFIDLLLMLGTRPQFSIQSAYITWSLAVSFLSSF
                     IFVLIFSQFKQHKLSQLLFIISSTLAISFIHLLTTLSLVSYNYFETDYLLFFTSMSLC
                     LVSSLLLNEFSFKNQTFYDYKYYIALLLFSLCLVISYRINLSAFDVFSVPSLVTDSVL
                     SQKALTAIAIVLFLVIINILLLISLHSYQKLKNQQQALDSAEQQLQKLQGNHSSLEQL
                     AHYDTLTGLFNRHAFMDAFAARLTESKQSANKLAIMFIDLDNFKRINDSLGHSAGDEL
                     LRIISRRLRSVLRGHDLIGRIGGDEFCLVAPIGTTPEAKVIANRILHKMQEPITLSGH
                     VANTTISIGISLFPYDGDNQELLIKNADHALYQSKGSGRNTLNFYSDYLQHKSHRELS
                     LQKDLHSAITESQLFVEYQPVIDLKTKKLVSLEALVRWLHPQKGILTPEHFINIAEFN
                     GFVALVDMWVIRQICRDIKALQALGSPLMISMNCSALNINNDNFIPESLAILDEEKID
                     YQWFCFELSESVLYEHRHKAPIFLSKLHESGLKLIVDDFGSGASSLIWLKTLPITQLK
                     LDRSLLLDPNNPEDKEIVAALIAMSHKLGWQVTAKGIELSEQIELLNTELCDYAQGYM
                     FGKPMPFTKIIELLGK"
     gene            3657..>4246
                     /locus_tag="PLY05_07170"
     CDS             3657..>4246
                     /locus_tag="PLY05_07170"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HNC03120.1"
                     /translation="MTLADRVFAQIQDAIVKGQLKAGVKMSEAELTARYGVSRGPLRE
                     ALRRLEARKLLTRIPHVGVRVVELTIDDVLQMYQVREVLEGMAARLAAEHVTEEEVQG
                     LKQLLQQHQQQTELQTGKGYYQEEGDFDFHYRIVKASRNEVLMQMLCGELYHRVRLYR
                     YQFSVTEGRPHKAFAEHSRIIEAIEAKDGEMAELLMR"
BASE COUNT         1301 a          669 c          849 g         1427 t
ORIGIN      
        1 actgttgtaa ccaatacctg ttgataatgt atctaattga cgattacgag ccaattccgc
       61 atcgcgagca gaagaatata agcgtaaaat ttttgcatct tcttgttgct gtttaaaagc
      121 attgtctcgc ttagctttat ctttttgata tacagcttca tcaaatgggg gaatacgtcg
      181 gatgacttgc atttgcctat caacaatatc gtaaccacga cgaatgtgtt cttcactaac
      241 ttggtcactg acagttggta tgcctttgtc attatagtag cgataaagcc gtttattgtc
      301 ggagaagtca ttagcaagac ttgtagagct taaacaaagt agcaagataa ttgatgagta
      361 aatcggtttg ttttgcataa aaaacggcca ataaggtttg tgtggtttta ttttatttag
      421 catattctgt aaaaaatcat caattaaaat agtaaaggtt tgttattatc tgtaatccct
      481 caatcttaat atagctattt ccttttgtta aggttttatt tgtttgttct gtggaaacta
      541 tttttgtgcc attaatcgac tccctcattt tgtttgtgat ttctttagtt gcaaatttat
      601 tatctgcttt agcgggtggg ggggcaggtt tattacaatt gcctgcgtta ttgcatcttg
      661 gtttaccttt tggtatggca ttggcaacgc acaagattgc cagtgttgct ttaggggttg
      721 gtgcaacaac acgccattgg caaacaggta aattaaaatg gcgttttgcg ctgttaattt
      781 tgcttgctgg tttacctgga gtttggctag gaacacaatt aattttagcc attcctgaac
      841 aaatagccca gtttgctttg ggggttttaa ccttagggtt aggcgtttat tctttattaa
      901 aaaaagattt aggattaaca agtcagcgta ttcatcgaga taaaataggc ttaatcatag
      961 gctgtttagg tttgtttatt atagggatat tgaatggttc tttgacatca gggactggtt
     1021 tatttgtgac tatctggtta gttcgatggt ttggttttga ttttacccgt gcggtatctt
     1081 ataccttgat tttagtcggt tttttttgga atggtttagg tgcattattt ttgggcttac
     1141 aaacacctgt cgcttggtct tggttacctg ccttattatt ggggtcttta attggtgggt
     1201 atcttggtgc tcattgggct ttattaaagg gtaatcaatt agtgaagcgt agttttgaag
     1261 tgttgacaat ccttgtcggt ctaagcttat tatttaccta gtaattcaat gattttagta
     1321 aatggcatgg gtttaccaaa catatatcct tgagcgtagt cacaaagttc ggtgtttaat
     1381 aattcaattt gttcggataa ttcaatccct tttgcggtta cttgccaacc aagtttatga
     1441 gacatagcaa ttaaagctgc aacaatttct ttatcttcag ggttgttggg gtctaataat
     1501 aaactacgat ctagttttaa ttgcgtgata ggtaatgttt ttaaccatat caatgaagat
     1561 gctcctgaac caaaatcatc gacaattaat tttagtccag attcatgcag tttagataaa
     1621 aaaatcggag ctttatgtcg atgctcatac aaaacgcttt cagataattc aaaacaaaac
     1681 cattgataat caattttttc ttcatcaaga atggcaagac tttctgggat aaaattatca
     1741 ttattaatat tgagagctga gcaattcata ctaatcatta acggcgaacc taaagcttgt
     1801 aatgctttga tatcacggca aatttgacga ataacccaca tatcaactaa ggcaacaaaa
     1861 ccgttaaatt ctgcaatatt aataaaatgt tcaggtgtta gaatgccttt ttgtggatgt
     1921 agccaacgca ctagggcttc taagctaact agttttttgg tttttaaatc aataacgggt
     1981 tggtattcca caaaaagctg agattcagta atggcagaat gtaggtcttt ttgtaaagaa
     2041 agctcccgat gtgatttgtg ttgtaaataa tcactgtaaa agtttaaagt atttcgacct
     2101 gaaccttttg attgataaag tgcgtgatca gcatttttga tgagtaattc ttgattgtca
     2161 ccatcatagg gaaataagct aatgccaata ctgatcgttg tattggcaac atgcccactc
     2221 aaggtaattg gctcttgcat tttatgcaat atacgatttg caatcacttt tgcttcgggt
     2281 gttgtaccaa tgggtgcaac tagacaaaac tcatctccgc caattctgcc aattaagtca
     2341 tgtccacgta aaacacttct taatcgtcga gaaataattc tcagcaattc atcgcctgca
     2401 gagtggccta aggagtcatt gattcgttta aaattgtcta aatcaataaa catgatcgct
     2461 aatttattgg ctgattgctt agattccgtt aaacgagcgg caaaggcatc cataaaggca
     2521 tgacgattaa ataaaccagt taatgtgtcg taatgagcga gttgctcaag agaggaatga
     2581 ttaccttgta atttttgtaa ttgttgttcg gcactgtcga gtgcttgttg ttgatttttg
     2641 agtttttgat aactatgtaa tgatatcaac aataaaatat taataattac taaaaacaaa
     2701 acaatggcta ttgctgttaa agctttttgt gataatacgc tatctgtgac taaacttggc
     2761 acgctgaaaa cgtcaaaagc acttaaattt atacgatatg aaatgaccaa acataaacta
     2821 aataataata aagctatgta gtatttatag tcataaaatg tttgattttt aaaagaaaat
     2881 tcatttagta aaaggctgct aactaagcat aaactcattg aggtaaaaaa gagtaaatag
     2941 tctgtttcaa aatagttata gctgactaag ctcagcgtgg ttaataaatg gatgaatgat
     3001 atggctaagg tgcttgatat aataaacaat agttggctta acttgtgctg tttaaattga
     3061 ctaaaaataa gcacaaaaat aaaactactc aaaaagctaa ccgccaatga ccatgtgata
     3121 tatgcgcttt gaatggaaaa ttgtggacga gtacctaaca ttaatagcag atcaataaac
     3181 cagatattta atgctaatac ggtattgatg attaaatacc aaattaaatt caatggtgaa
     3241 cgatctttga ataaagagtg gtgatgttgg ctgattgtgg tgatgctata aaaatgccct
     3301 ccagcgcaaa ttaaggcaaa aatgacaaat aaaggcgata tttgccaagt taatgcttca
     3361 gtaatagaag ggctaagtgt agtcatataa aagagacttc cttgaatctg aatagtttta
     3421 aaacgaataa tgcagtaaaa aattttgtcg attgttgact aaattacatt aatcatcaag
     3481 catattttaa atagtcattc ttatgctgag aatttcattt ttttgtcgac aaaatattag
     3541 tttttatttg actgtttgtc ttttttgctt attattgctc tactttttat aagattgtag
     3601 acaatattgt gcagcaagct ccttcttcaa tcatagaatc ttcaaatgaa gctaatatga
     3661 ctttagccga ccgagtattt gcccaaattc aggatgcgat tgttaaagga cagctcaaag
     3721 ctggcgtaaa aatgtccgaa gcggagctaa ccgcccgtta tggtgtttca cggggtccat
     3781 tgcgcgaagc cttacgccgt ttggaagcac gaaaattatt gacccgtatt cctcatgtgg
     3841 gagtgcgtgt tgttgaacta accattgatg atgtgttgca gatgtaccaa gtgcgtgaag
     3901 tcttggaagg catggctgca cgtttggcgg ctgagcatgt taccgaagaa gaagtacaag
     3961 gcttaaagca actgttgcaa cagcaccaac aacaaaccga attacaaaca ggtaagggct
     4021 attatcaaga agaaggtgat tttgattttc actatcggat tgtaaaggcc agtcgtaatg
     4081 aagtgttgat gcaaatgctc tgtggtgagt tatatcatcg ggtgcgtttg tatcgttatc
     4141 agttttctgt gaccgaaggt cgtccccata aggcatttgc cgaacattcc cgaattattg
     4201 aagccattga agccaaagac ggtgaaatgg ctgagttgtt aatgcg
//
LOCUS       DAOQMB010000190         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10199_length_4241_cov_15.604396, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000190 DAOQMB010000000
VERSION     DAOQMB010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10199_length_4241_cov_15.604396"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="groEL"
                     /locus_tag="PLY05_07175"
     CDS             complement(<1..905)
                     /gene="groEL"
                     /locus_tag="PLY05_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNC03121.1"
                     /translation="MAAKDVKFGDSARQKMINGVNILADAVKVTLGPKGRNVVIDKAF
                     GAPHITKDGVSVAKEIELKDKFENMGAQLVKEVASKTADVAGDGTTTATVLAQAILNE
                     GLKAVAAGMNPMDLKRGIDKAVRAAVAEIKAIATPCADSKAVAQVGSISANSDTTIGD
                     LIAQAMDKVGKEGVITVEEGSGFEDTLEVVEGMQFDRGYISPYFINKQDSMTAELDSP
                     FILLVDKKISNIRELIPVLEGVAKSSKPLLLIAEDVEGEALATLVVNSMRGIVKVCAV
                     KAPGFGDRRKAMLQDIAILTGGQVIA"
     gene            complement(948..1238)
                     /locus_tag="PLY05_07180"
     CDS             complement(948..1238)
                     /locus_tag="PLY05_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNC03122.1"
                     /translation="MKIRPLHDRVVIRREEQETKTAGGIVLPGSAAEKPSQGEVIAVG
                     NGQITESGEVRPLDVKVGDKVLFGQYSGSTVKVDGEELLIMKESEILGVLEA"
     gene            complement(1391..1993)
                     /locus_tag="PLY05_07185"
     CDS             complement(1391..1993)
                     /locus_tag="PLY05_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HNC03123.1"
                     /translation="MALLLVIGFLIADIVLSIWVGKQVGGWMLLLWFVMAFIVGRQIM
                     KGASKELTPQLQQAQMGGAVDPNANFLAAICQALAGFLLILPSVLTDLMAVLLLLPPV
                     QKALQGKMQQAFAARGANLMMMGGFGRAGQSPFGQQSPFGNNPFAKGDVFDGEATEVK
                     AKPTPAVSNMRVTTATQKPIQTSTEVKGGMKVTTRLDKNN"
     gene            complement(1996..2700)
                     /locus_tag="PLY05_07190"
     CDS             complement(1996..2700)
                     /locus_tag="PLY05_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HNC03124.1"
                     /translation="MANSELQQLLLQSYQGVLSTHSQHLQGYPFGSVVPFCLDYQGRV
                     IILISELAQHTKNLKVDKCCSLLVFAEGDDIQAAARLTIIADATPVAQHEVETIAQRY
                     YRYFPQSKDFHNIHDFSFWVLQPVKYRYIGGFGKIHWAEPQTLANPFNAETETDMVEH
                     MNQDHADALPLYAKLANIVANNVTISMTGIDSLGFHLRIGEKIIYISFPQAVNSPQQV
                     RQTLVELLKIARQTGN"
     gene            2813..4090
                     /locus_tag="PLY05_07195"
     CDS             2813..4090
                     /locus_tag="PLY05_07195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC03125.1"
                     /translation="MTNPTTRTYTLKEAFAQPAALIMFFFGFTSGLPFLLVGGTLSAW
                     LKDSQVSLEMIGLISYASLTYSLKFLWSPFVDQAKLPILHKLGQRRSWLLLAQIMLCT
                     SLLAMSVISPHALTLFVLFVFLAAFAGATQDIVVDAYRIEIAPAEIQGALAATYTLGY
                     RFALLASGALALMLADHVSWPLIYQLMAGLVILMMLATYLAKEPQRQQSTFNSFGEMM
                     TDGVIGPFKDFFARYKGALGVGLLLFMGLFKIADQMLGVMAIPFYLDSGFTKTQIGAV
                     SKLFGVWVGIAGAFIGGASVVRFGIERTLFVGMVVGALSNLLFILLSVFPANINIFVL
                     VIGGENFAGGFLGTAAVAWLSSLVNQHYTATQYALFSSLVTLPGKLIGGLSGFMVTSI
                     GYVGFFVFSTVSILPALILFIWLRKKIQVNKFY"
BASE COUNT         1234 a          872 c          774 g         1361 t
ORIGIN      
        1 gcaatcactt gaccgccagt taagatagcg atgtcttgta acatagcttt acgacggtcg
       61 ccaaaacctg gggctttaac ggcacacact ttaacaatac cacgcatgct gttaacaact
      121 aacgttgcta aggcttcgcc ttctacatct tcagcaatta ataacaatgg cttgctagac
      181 ttagcaacac cttctaatac aggaatcaat tcacggatat tagagatttt tttgtcaacc
      241 aataaaatga atggagagtc taactcagca gtcatagaat cttgtttgtt gatgaagtat
      301 gggctgatgt aaccgcgatc aaactgcata ccttcaacca cttctaaggt atcttcaaaa
      361 ccagaacctt cttcaacggt gataacgcct tctttgccca ctttatccat ggcttgagca
      421 atcaaatcac caatagttgt gtcagagtta gcagaaatag aacctacttg agcaacggct
      481 ttgctgtcag cacaaggtgt tgcaatcgct ttgatttcag caacagcagc acgaacagct
      541 ttgtcgatac cgcgttttaa atccattgga ttcatgccag cagcaacggc ttttaaacct
      601 tcatttaaaa tagcttgagc taatacagta gcagttgttg taccgtcacc agccacatca
      661 gcagttttag aagcaacttc tttaactaac tgtgcgccca tgttttcgaa cttgtctttt
      721 aattcgattt ctttagcaac agagacacca tctttagtga tgtgtggtgc gccgaaagct
      781 ttgtcgatga caacgttacg gccttttgga cctaaagtta ctttaacagc atcagctaaa
      841 atgtttacgc cattaatcat tttttgacga gcggagtcac cgaattttac gtctttagca
      901 gccatttttg actaacctca aaatacttaa tattaacaat ccaagaatta ggcttctaat
      961 acgcctaaaa tttctgattc tttcataatc aataattctt caccgtcaac tttaacagtg
     1021 ctaccagaat attgaccaaa taatactttg tcgcccactt tgacatctaa aggacgaact
     1081 tcgccagact cagtgatttg accattacct actgcaatca cttcaccttg agaaggtttt
     1141 tcagcagcag aacccggcaa tacgataccg ccagcggttt tggtttcttg ctcttcacgg
     1201 cgaataacca cacggtcatg taaaggacga attttcattg ttcgcgctcc tgaatgcgtt
     1261 acatatatca ttagattaaa aactcactgc gtatagcagc gagactgtga aaaaatgggg
     1321 acagctttac cagcttcaag gggaaaaaat aaaaataaga gcagatagac cttatccgct
     1381 caaaaaatag tcagttattt ttatcaagtc gagtagtgac tttcatgcca cctttgactt
     1441 ctgttgacgt ttgtataggt ttttgagttg ctgtagtcac acgcatatta cttacggctg
     1501 gtgttggttt agctttaact tcagttgcct caccatcaaa gacatcacct ttcgcaaagg
     1561 gattattacc aaaaggcgat tgttgtccaa atggtgactg gcctgctcga ccaaaaccac
     1621 ccatcatcat taaatttgca ccacgcgctg cgaaagcttg ttgcatttta ccttgtaatg
     1681 ctttttgcac aggtggtaac aataacagta ccgccatcaa atcagtgagt acactgggca
     1741 aaatcaataa aaagccagct aatgcctgac aaattgcagc aagaaaattg gcattgggat
     1801 caactgcccc acccatttgt gcttgttgta attggggagt taactctttg cttgcacctt
     1861 tcataatttg acgaccgaca ataaaagcca tcacaaacca taatagcagc atccaaccac
     1921 ccacttgttt accgacccaa atactcaaaa cgatatcggc gattaaaaaa ccaattacta
     1981 ataataaagc catgtttaat ttcctgtttg acgcgctatt tttaataact caactaatgt
     2041 ttgtcgtact tgctgtggac tattgactgc ttgtgggaag ctaatataaa taattttttc
     2101 gccaatgcgt aaatgaaagc ccaaactatc aatacccgtc attgatatag tgacattatt
     2161 tgcaacaata ttggctagtt tagcatataa cggtaaagca tcagcatggt cttgattcat
     2221 atgctcaacc atatcagtct ctgtttctgc attaaaagga ttcgccaaag tttgcggttc
     2281 tgcccaatga attttgccaa aaccaccaat ataacggtat ttgacgggtt gtaagaccca
     2341 aaaactaaaa tcgtgaatgt tgtggaaatc ttttgattga gggaaataac gataataacg
     2401 ctgagcgatt gtctctacct catgttgagc aacaggcgta gcatcagcaa taatggttaa
     2461 ccttgctgct gcttgaatat catcaccctc agcaaacacc aacaaagaac aacatttatc
     2521 aacttttaaa tttttagtat gttgagctaa ctcagaaatt aaaataatga ctcgcccttg
     2581 atagtctaaa caaaatggca caacagagcc aaaaggatag ccttgcaaat gttgagaatg
     2641 ggtacttaac accccttgat aactttgtaa caataattgt tgcaattcgc tatttgccat
     2701 aacttcaccc aaacttgatc tttttcaaga ttatcataaa gcgatctttg ccatctgtta
     2761 ttgttttgca ttactctctg cccatcattc ataaaattta acttcaacca tcatgactaa
     2821 tcctacaact cgaacctata ccctcaagga agcctttgcc caacctgcgg cattgattat
     2881 gttctttttt ggctttacct ctggtttacc ctttttattg gtcggaggca cattatctgc
     2941 atggttaaaa gatagccaag tttctttaga gatgattggc ttaattagtt atgcatcttt
     3001 aacttattcg ctaaaatttt tatggtcacc ctttgtagat caagcaaaat tacctatttt
     3061 gcataaacta ggtcaacggc gtagttggct attactggca caaattatgt tatgtactag
     3121 cttattggcg atgtcagtta ttagtcctca cgccttaaca ttgttcgtct tatttgtttt
     3181 tttagccgca tttgcaggcg caactcaaga cattgttgtc gatgcttacc gtattgaaat
     3241 tgcacctgcc gaaatacagg gggcattagc cgccacttat acgctaggtt atcgttttgc
     3301 tttacttgct tcgggagcgt tagctttgat gttagctgac catgtctcgt ggccattaat
     3361 ttaccaattg atggcaggtt tagtcatatt aatgatgctt gccacttatt tagccaaaga
     3421 accacagcgt caacaaagta ccttcaactc ttttggcgaa atgatgactg atggggtgat
     3481 tggccctttt aaagactttt ttgctcgata taaaggtgct ttaggggttg gtctattact
     3541 atttatgggc ttatttaaaa tagccgacca aatgttaggt gtcatggcta ttccctttta
     3601 tttagatagt ggttttacta aaactcaaat tggtgcagtt tccaaattat ttggcgtatg
     3661 ggtaggtatt gcgggtgcat ttattggtgg tgcaagtgtg gttcgatttg ggattgaacg
     3721 cactttattt gtgggcatgg tcgtcggtgc attaagtaac ttattgttta ttttattgag
     3781 tgtatttcct gccaatatta atatttttgt cttagtcatt ggtggtgaaa actttgctgg
     3841 tggatttttg ggaacagccg ctgttgcatg gttatcatct ttagttaatc aacactatac
     3901 cgccacccaa tatgctttat ttagctcttt agtgacttta cctggtaaat taattggtgg
     3961 actatcaggc tttatggtga caagcatagg ctatgtagga ttttttgttt tttcgactgt
     4021 ctctattttg cctgcgttaa tactatttat atggttgcgt aaaaaaatac aggttaataa
     4081 attttattaa caatcaaaca acagcaaaaa caagctttta agctatctta tcaaagagac
     4141 ttcggctgtt tgttaacagc cgatttaata ataacaacag aggaactcta atctatgaaa
     4201 ctttatccac gttatttagc cattgcgatt agtttattaa g
//
LOCUS       DAOQMB010000191         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10234_length_4231_cov_11.510536, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000191 DAOQMB010000000
VERSION     DAOQMB010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10234_length_4231_cov_11.510536"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLY05_07200"
     CDS             <1..534
                     /locus_tag="PLY05_07200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HNC03126.1"
                     /translation="VEKVLALGQGKQWRVVDLGTGSGAIALSLAKEHPEWAVVATDLY
                     PQSLAIAQQNAEKNQITNVEFVQGSWFEPLTGLFDCIVSNPPYIIENDPHLQALSHEP
                     QRALVAAEQGLADLRHIVQLSGKFLCDQGYLVLEHGFDQGQAVRDLLQASGFSQIMTG
                     QDFGGNDRYTVARLHKS"
     gene            547..>1295
                     /gene="moeB"
                     /locus_tag="PLY05_07205"
     CDS             547..>1295
                     /gene="moeB"
                     /locus_tag="PLY05_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HNC03127.1"
                     /translation="MLTDDELLRYNRQIMLPQWDIAAQERIKASKALIVGMGGLGCPA
                     ALYLASAGVGELWLADFDTVEVSNLQRQVLYRDSDIGSPKAQAAAKHLQQVNPYIVLR
                     PMLEALNVDNLLAIVQQVDVVLDCSDNFTTRDAVNTACVQANKPLISAAAIGLSGQLS
                     VFDGGHEHRPCYRCLYPQADEQSMTCSESGVLATLVGVMGTLQAHEALKCLSGLGEGL
                     YGKLLLWDADMPTWRTLKFRKDPACPVCGQN"
     assembly_gap    1296..1395
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1397..2722
                     /locus_tag="PLY05_07210"
     CDS             1397..2722
                     /locus_tag="PLY05_07210"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNC03128.1"
                     /translation="MNRYATFGNALKGIVRIGETTAILTTTGLSWLRGNRPPTPRLVR
                     QTFERLGATYVKLGQFVASSPSLFPPEYVNEFQYCLDKTQALPFKYIRQVIEEELKQP
                     LDKVFASVDETPLASASIAQVHAATLITGESVVIKVQKPDVKNILLTDLNFIYWATRI
                     FERLFPKIKFASLSAIVSEIQECMMEEVDFYKEAENIKQFNEFLKATNNPLAIAPQVY
                     PQASSLRVLTMERFYGVPLTDLETIKRYAKDPANTLINAMNTWFSSLMFCESFHADLH
                     AGNLMVLTDGRIGFIDFGIVGRVKPDTWSATMAFMESIGTGDFEKMADSMMKIGMTSQ
                     KVNTTDLAKDLEVIYQAFTVVDPTSVLNGAVNDNEINQLMLEIVAVGEKHGIRFPRAF
                     ALLFKQILYFDRYIKILAPDMELFNDDRLQMFGSLEDLSGKKPSPRVLH"
     gene            complement(2739..4172)
                     /gene="phrB"
                     /locus_tag="PLY05_07215"
     CDS             complement(2739..4172)
                     /gene="phrB"
                     /locus_tag="PLY05_07215"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF007955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNC03129.1"
                     /translation="MKHLMWFRADLRTIDNTALTAACQHPQAEVIGLYFISAEQWQKH
                     HTAGCKVEFELATVQQLSNRLQQLNIPLIVINSPNFNSQITDLVKFCQKENITHVFYN
                     TQYEINELQRDAQIEQQLRQLNVQVSSFDDQCIVTPSRVLTNDGRFYTVFTPFKKTWL
                     QQINIGGIKVLAAPQPRAHRAGQQATPIPSHLNSFSSHISQETAQLYWPAGEEAALKR
                     LDIFCQHHIRGYKDNRDYPLHPEGTSRLSPYLAVGSISARQCFQAAINEQQAYGHSDG
                     IDCWISELAWRDFYKHLMVAYPHLCKHKAFKANTDNIVWRHDQADFQRWCEGKTGFPI
                     VDAAMRQLNTIGWMHNRLRMVTAMFLTKDLFIDWRWGERYFMEHLIDGDLAANNGGWQ
                     WSASTGNDAAPYFRIFNPALQSQKFDANGDFIRRYVPELAHLDSKTIHEPHGKQRQLF
                     LDYPLPMVNHKQACAFAVEQFEKIKAT"
BASE COUNT         1201 a          761 c          898 g         1271 t
ORIGIN      
        1 gtcgaaaaag tcttggcttt ggggcagggt aaacagtggc gcgtggttga tttgggcaca
       61 ggcagtggtg cgattgcctt aagccttgcc aaagaacacc ctgaatgggc tgtggtagca
      121 actgatttat atccgcaaag tttagcaatt gctcaacaaa atgctgaaaa aaatcaaatt
      181 accaatgttg aatttgtgca gggttcgtgg tttgagccgt taacaggttt atttgattgt
      241 atagtcagta atccccctta tattatcgaa aatgatcccc atttacaggc attaagtcac
      301 gagccacaac gcgctttggt agccgctgaa caaggtttag ccgatttgcg acatattgtc
      361 caattgtctg gcaagttttt atgcgatcaa ggttatttag tgttagaaca tgggtttgac
      421 caaggccaag cggtgcgtga tttgttacaa gcatcaggat ttagccaaat tatgacaggc
      481 caagattttg gtggaaatga cagatatacc gtagcaagac tgcataagtc ttaaatagag
      541 ccaaaaatgc ttacagatga cgagttactc cgctataacc gccaaattat gttgccccaa
      601 tgggatattg ctgctcaaga acgcattaaa gcctctaaag ctttgattgt cggtatgggc
      661 ggtttgggtt gtcctgctgc tttatatttg gccagtgcgg gtgtgggcga gttgtggctt
      721 gccgactttg atacggtgga agtttctaat ttacaacggc aagtgttata tcgagatagc
      781 gatattggct caccaaaagc ccaagcagcg gctaaacatt tgcaacaagt gaatccatat
      841 attgtcctta ggccaatgct tgaggcttta aatgtcgata acttattggc cattgtgcaa
      901 caggttgatg tggtattgga ctgtagcgac aattttacca cacgtgatgc agtgaatacg
      961 gcttgtgtac aagccaataa accattgatt tcggcagctg ccattgggtt gagtggccaa
     1021 ctgtcggtgt ttgatggtgg gcatgaacat cgtccttgtt atcgctgttt gtatcctcaa
     1081 gcagatgaac aaagtatgac atgcagcgag tctggtgtat tggcgacctt agttggcgtc
     1141 atgggaactt tacaagcaca tgaggcatta aaatgtttaa gcggtttagg agaggggttg
     1201 tacggcaagt tactactatg ggacgccgat atgcccacat ggcgcacact caaatttcgc
     1261 aaagatcccg cttgtcccgt gtgtggccaa aacaannnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnaatga atcgttatgc aacgtttggt aatgctttaa aagggattgt
     1441 tcgcattggt gaaacaacgg ccattttaac aaccacagga ttaagttggt tgcgtggtaa
     1501 tcgtccacct acacctcgtt tagttcgcca aacctttgaa agattagggg caacatacgt
     1561 taagttaggc cagtttgtgg ctagttcacc ctcattattt ccacctgaat atgtcaatga
     1621 atttcagtat tgtttagata agacgcaagc tttacccttt aaatatattc gccaagtgat
     1681 tgaagaagag ttaaaacaac ccttagacaa agtgtttgcc agtgttgatg aaacgccatt
     1741 agcttctgct tctattgctc aagttcatgc agcaacctta ataacaggtg agtcagttgt
     1801 tattaaagtg caaaagcctg atgttaaaaa tattttatta acagatttaa attttattta
     1861 ttgggcaaca cgtatttttg aacgtttatt tccaaaaatt aaatttgctt cgttatcagc
     1921 catcgtatcg gaaattcaag aatgtatgat ggaagaggtt gatttttata aagaagccga
     1981 aaacatcaaa caatttaacg agtttttaaa agctaccaat aatcctttag ccattgcgcc
     2041 acaagtgtac ccacaagcat ccagcttgcg tgtgttaacg atggaacgtt tttatggtgt
     2101 gccattaacc gatttagaaa ccattaagcg ttatgctaaa gatcctgcaa acactttaat
     2161 taatgccatg aacacttggt tttctagttt aatgttttgt gaatctttcc atgcggattt
     2221 acatgcaggt aacttaatgg tgttaaccga tggccgtatt ggttttattg attttggcat
     2281 tgtcggtcga gttaagccag atacatggtc agcaacgatg gcttttatgg agtcaattgg
     2341 cactggtgac tttgaaaaaa tggccgattc gatgatgaaa attggtatga ccagccaaaa
     2401 agttaacaca actgatttag ccaaagattt agaagtgatt tatcaagctt ttaccgtggt
     2461 tgatccaacc agtgtgttaa atggcgcggt taacgacaat gaaattaatc aattgatgtt
     2521 ggaaattgtc gccgttggtg aaaagcacgg cattcgtttc ccacgtgcgt ttgctttgtt
     2581 atttaagcaa attttatatt ttgatcgtta tatcaaaatt ttagcaccag acatggaatt
     2641 gtttaatgat gatcgtttgc aaatgtttgg cagtttggaa gatttaagtg gtaaaaaacc
     2701 aagccccaga gtgttacatt gaaacattta aaagcgactt aggtcgcttt tattttttca
     2761 aactgctcaa ctgcaaaggc acacgcttgt ttatgattta ccatcggtaa tgggtaatct
     2821 aaaaataatt gtcgttgttt gccgtgtggt tcgtgaatgg ttttgctgtc taaatgagcg
     2881 agttcaggca cataacggcg aatgaaatcg ccattggcat caaacttttg gctttgtagc
     2941 gcagggttaa aaattctaaa atagggtgct gcatcattac ctgttgatgc gctccattgc
     3001 caaccgccat tattggctgc caaatcacca tcaattaaat gttccataaa ataacgttcg
     3061 ccccaacgcc aatctataaa caaatctttg gttaaaaaca tggctgttac catgcgtaaa
     3121 cggttgtgca tccaaccaat ggtgtttagt tggcgcattg ctgcatcaac aatcggaaaa
     3181 ccggtttttc cttcacacca gcgttgaaag tcggcttggt catgtcgcca gacaatatta
     3241 tcagtgttgg ctttaaaggc tttatgtttg cataaatgtg gataagcaac cattaagtgt
     3301 ttataaaaat cacgccatgc taattcgcta atccaacaat caataccgtc actatgacca
     3361 taagcttgtt gctcattaat tgccgcttga aaacattgtc tagcagaaat cgaaccaact
     3421 gctaaataag gcgataaacg gctagtacct tcgggatgta atgggtaatc acgattatct
     3481 ttataaccac gaatatgatg ttggcaaaaa atatctaaac gttttagagc cgcttcttca
     3541 cccgcaggcc aataaagctg tgccgtttct tgactaatgt gcgagctaaa tgaatttaaa
     3601 tgtgagggaa tgggggtggc ttgttgtcca gcgcgatgtg ctcgaggctg aggtgcggct
     3661 aatactttaa ttccaccaat attaatttgt tgtaaccacg tttttttaaa gggtgtgaaa
     3721 acggtataaa aacgcccatc attggttaat acacgcgatg gtgtaacaat acattggtca
     3781 tcaaaactag atacctgaac attcaattgt ctcagttgtt gctcaatttg agcatcacgt
     3841 tgtaattcat taatttcgta ttgtgtatta taaaaaacat gagtgatgtt ttctttttga
     3901 caaaatttaa ctaaatcagt tatttgggag ttaaagttag ggctatttat aacaattaac
     3961 ggaatattta attgctgtaa acggttgcta agttgttgaa cagtcgctaa ttcaaattca
     4021 actttacagc cagccgtatg gtgtttttgc cattgttccg cactgataaa atataagccg
     4081 atcacctctg cttgtggatg ttgacaagca gcggtgagag cagtgttatc aatggtgcgt
     4141 aaatcggcac gaaaccacat taaatgcttc atgtttatta cctaacgata ggtcttaaat
     4201 ctaaaccttt ttgtctgcgc cattgcaaaa c
//
LOCUS       DAOQMB010000192         4191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10395_length_4191_cov_12.772002, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000192 DAOQMB010000000
VERSION     DAOQMB010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_4191_cov_12.772002"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..1373
                     /gene="hisS"
                     /locus_tag="PLY05_07220"
     CDS             81..1373
                     /gene="hisS"
                     /locus_tag="PLY05_07220"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNC03130.1"
                     /translation="MSVKITAIRGMNDILPIPTKENPVTTQRWQAVEKVLRQLMHQYG
                     YKEIRLPIVENTPLFKRAIGEVTDIVEKEMYTFEDKGGDSVTLRPEGTAGCVRACLEH
                     GLTYNQTQRLWYTGPMFRYERPQKGRYRQFHQFGVETFGMKSADIDAELILMTARLWR
                     ELGLSEVVRLELNTLGESASRQAYKDALVAYLSQHLDSLDEDSKRRLNTNPLRILDSK
                     DPKTQAVLANAPQLFDYLDEESLQHFASLRAVLDAAGVAYTVNPRLVRGLDYYNKTVF
                     EWVTNALGAQGTVCAGGRYDGLVTQLGGQATPAVGFAIGLERLVLLLDTLQQQNDSSE
                     ADIYLVSEANTINQALLLAELLRNSLTKYRIVSHCGGGQFKNQLKRADKTGAQAALLL
                     GENEVAEQKVTVKWLQTGEQQQVNQADICTYLAQHLGA"
     gene            1376..2071
                     /locus_tag="PLY05_07225"
     CDS             1376..2071
                     /locus_tag="PLY05_07225"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC03131.1"
                     /translation="MSTHDDEQLENLKRFWQDYGTPIIVGASIALAVFAGWRYWQQSQ
                     LQTTALAANAYQASQDAYQKLAANSDDKAANTDLQREGQKLMQEFSKTPYATNAAFLL
                     AKRAIELKDYKEAEKHLRFVLNQEKGDEGLKSIATIRLAMVLSEKGDNKGALTLLNTE
                     TNPAFTPSREELRGDLLKANGDIAGAQKAYQAAMTALVERKEPRPLLEMKMADVGLEM
                     TEIKRPSPVKEPS"
     gene            2219..>2675
                     /locus_tag="PLY05_07230"
     CDS             2219..>2675
                     /locus_tag="PLY05_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC03132.1"
                     /translation="MIKRLSLAAIVIAVAACSSNPNEHKPSPLPVLPAKHEQIKLIQK
                     QDWRVGKGLIANEVAPQLVSQNNMLYVANHRGLVMAIEANQGKSWQQDIKQAVTGLQA
                     AYGRVVLSSAKGDIYVLDATTGQPRWQKNLNAPILAAATQTPERVIVQSN"
     assembly_gap    2676..2775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2776..3519
                     /locus_tag="PLY05_07235"
     CDS             <2776..3519
                     /locus_tag="PLY05_07235"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNC03133.1"
                     /translation="PILAAATQTPERVIVQSNDGKVYGLDAKTGNTVWVFDTPTAPLS
                     LRGYASPLVIDDQVIVAGATGKIFALEAQTGIALWESRVASSNGRSELERMVDVDGQM
                     LVDSEQKLYVASYQGQLSALALQADKKPVPLWQENLSSYNSLAEGLGNIYAVDSSDHI
                     VAFDKQTGQVVWRQKDFAWRGLSQPVTANNLLFVGDKQGYVHIMAQSDGKLLGRFKVK
                     GAVADLAIFNQALWVYSQQGQLTTWPLPQ"
     gene            3593..>4191
                     /locus_tag="PLY05_07240"
     CDS             3593..>4191
                     /locus_tag="PLY05_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HNC03134.1"
                     /translation="MKPVIALIGRPNVGKSTLFNQLTKTRNALVANLPGLTRDRQYGD
                     GHFENKSFIVIDTGGLGESDEGIDAYMAEQAKTAIQEADIVLFVVDARAGLLGSDEMI
                     ADHLRRLNKQSFLVVNKVDGLHEDAATAEFHRLGFSRIFQTAASHGRGVFQLISDLLE
                     PFPEDEAELSPEETGIKIAVVGRPNVGKSTLVNRLLGED"
BASE COUNT         1235 a          765 c          916 g         1175 t
ORIGIN      
        1 gctcttgtta aatcttgctc attaatcata actggcgtaa attacgctgc ttttaattta
       61 gtccgtattg agaatccatc atgtccgtta aaatcactgc cattcgtggt atgaatgata
      121 ttttacctat ccccaccaaa gaaaatcctg ttactacaca acgttggcaa gcggttgaga
      181 aggtattaag acagttgatg catcaatatg gctataaaga aattcgtttg ccgattgttg
      241 aaaatacgcc tttgtttaaa cgagccattg gtgaagtcac tgatattgtt gaaaaagaaa
      301 tgtacacctt tgaagataaa ggtggtgact ctgtgacctt gcgtcctgaa ggtacagctg
      361 gttgtgtgcg tgcttgttta gaacacggtt taacctacaa ccaaactcaa cgtctatggt
      421 acacagggcc gatgtttcgt tatgagcgtc cacaaaaagg ccgctatcgt caattccatc
      481 aatttggtgt tgaaactttt ggcatgaaat cggcagatat tgatgccgaa ttaattttaa
      541 tgaccgcacg tttgtggcgt gaattaggtt tgagcgaagt tgttcgttta gagctaaata
      601 ctttaggcga aagtgcgtct cgtcaagctt ataaagatgc gttggtggct tatttaagtc
      661 aacatcttga tagtttagat gaagattcaa aacggcgttt aaacaccaat cctttgcgta
      721 ttttagacag caaagatccc aaaacgcagg cagttttagc caatgctcct caattatttg
      781 attatttaga tgaagaatct ttacaacatt ttgccagttt aagagccgta ttagatgcgg
      841 ctggtgtcgc ctataccgtt aatcctcgtt tagtacgtgg tttggattat tacaacaaaa
      901 ctgtttttga atgggtaaca aacgcattag gtgcacaagg tacagtttgc gcgggtggtc
      961 gttatgatgg tttggtaaca caattgggtg gacaggcaac acctgccgtg ggttttgcca
     1021 ttggtttaga acgtttggtt ttgttattgg ataccttaca acaacaaaac gacagcagcg
     1081 aagcggatat ttaccttgtt tctgaagcaa ataccattaa tcaagcgttg ttgttagcag
     1141 aactactaag gaactcttta acaaagtatc gtattgtaag ccactgtggt ggtggtcaat
     1201 ttaagaatca attaaaacgc gccgataaaa cgggtgcaca agccgcctta ttattaggtg
     1261 aaaatgaagt cgctgaacaa aaagtcacgg ttaagtggtt acaaacaggc gaacagcaac
     1321 aagttaatca agcagatatt tgcacatatt tagcacagca tttaggagca taagcgtgag
     1381 tacacatgat gatgaacaac tagaaaacct caaacgcttt tggcaagact atggtacacc
     1441 gattattgtt ggggcaagta ttgcattagc agtatttgct ggttggcgtt attggcaaca
     1501 aagccaactc caaaccaccg cattagctgc aaatgcttat caagcctctc aagatgctta
     1561 tcaaaaatta gctgcaaact cagatgacaa agcagcaaat actgatttgc aacgtgaagg
     1621 tcaaaagtta atgcaagaat ttagtaaaac accttatgca acaaatgctg catttttgtt
     1681 ggctaaacgt gctattgaat taaaagacta taaagaagcc gaaaaacatt tgcgttttgt
     1741 gcttaatcaa gaaaaaggtg atgaaggttt aaaaagtatt gccactattc gcttggcgat
     1801 ggttttgtct gaaaaaggtg acaataaagg tgcattaact ttattaaata cagaaactaa
     1861 tccagcattt acgcctagtc gtgaagaatt gcgtggagat ttgttaaaag caaatggtga
     1921 tattgctggc gcacaaaaag cttatcaagc agcaatgact gctttagtag agcgcaaaga
     1981 gcctcgtccc ttgttagaaa tgaaaatggc agatgttggc ttagaaatga cagaaatcaa
     2041 acgtccttca cctgttaaag aaccttctta aaacctgtta gctccctcgc ccctttgggg
     2101 agaggggtaa gtagtagcct gtgtgaagta taacgtaaca caggacaaaa ttttgtaagc
     2161 attccctgta tttcgctacg ctacatacag gctactctta tcatcaagga gtttgtcgtt
     2221 gattaaacgt ctatccttag ctgccatcgt tatcgctgtt gccgcttgta gctctaatcc
     2281 taatgagcat aaaccttcac cgttgccagt attacctgcc aagcatgagc aaattaagct
     2341 aatacaaaag caggattggc gagtgggtaa aggcttaatt gcaaatgaag ttgctccaca
     2401 attagttagt caaaacaata tgttatatgt ggcaaaccat cgtggtttgg ttatggcaat
     2461 tgaggcaaat caaggcaaat catggcaaca agatattaaa caagctgtaa ctggcttaca
     2521 ggctgcttat ggacgagtag tattgtctag tgcaaagggt gatatttatg ttttagatgc
     2581 cactacggga caacctcgtt ggcaaaaaaa cttaaatgcc cctattttag cggctgctac
     2641 tcaaacgccc gaacgagtta ttgtgcaaag caacgnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnncctat tttagcggct gctactcaaa cgcccgaacg agttattgtg
     2821 caaagcaacg atggcaaagt gtatggcttg gacgcaaaaa cgggtaatac cgtatgggta
     2881 tttgacacac caaccgcgcc tttaagttta cgtggttatg ccagtccttt agtgattgat
     2941 gatcaagtga ttgtggcagg ggctacaggc aaaatctttg ctttagaagc tcaaacaggc
     3001 attgccttgt gggaaagtcg agttgcttct tctaatggac gctctgaatt ggagcgtatg
     3061 gtcgatgtag atggccaaat gttagtagat agtgagcaaa aattatatgt agcgagttat
     3121 caaggacagc tctccgcttt agctttacaa gccgataaaa agcctgtccc attgtggcaa
     3181 gagaatctat ctagctacaa ctcattagct gaaggtttag gtaatattta tgcggttgac
     3241 agcagtgatc atattgtggc ctttgataaa caaacaggcc aagtggtatg gcgacaaaaa
     3301 gactttgcat ggcgaggttt atcccaacca gtcactgcaa ataatttatt atttgtgggg
     3361 gataaacaag gttatgtgca tattatggcg caaagtgatg gtaaactatt aggccgtttt
     3421 aaggtaaaag gcgcggttgc cgatttagct atctttaatc aagccttatg ggtctatagt
     3481 caacaagggc aattaactac ttggccttta ccacaatagt ctcatttgac cccttctacc
     3541 agaaaaagaa ggggactaag cccgcatcgc ctgcatagtc gagttattag caatgaaacc
     3601 tgttattgcc ctcattggcc gtcccaacgt tggtaaatcc acattattta atcaattaac
     3661 caaaacccgt aacgctttag tggccaattt accgggactg actcgtgatc gtcagtatgg
     3721 tgatggccat tttgaaaaca aatcgtttat cgtgattgat acaggtggtt tgggcgagag
     3781 tgacgaaggc attgatgcct acatggcaga gcaagccaaa acagcgattc aagaagcgga
     3841 tattgtttta tttgtcgttg atgcacgcgc aggtttatta ggctctgacg aaatgattgc
     3901 cgatcattta cgccgcctta ataaacaaag ttttttggtc gtcaataaag tagatggttt
     3961 gcatgaagat gcagcgacag ccgaatttca tcgtttaggg tttagccgca tttttcaaac
     4021 cgccgctagt catggtcgtg gtgtttttca gttaattagt gatttattag aaccattccc
     4081 cgaagatgaa gccgaattat cacccgaaga aacagggatt aaaattgcgg ttgttggtcg
     4141 tcctaatgta ggtaagtcaa ccttagtcaa tcgtttatta ggcgaggaca g
//
LOCUS       DAOQMB010000193         4183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10425_length_4183_cov_16.990552, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000193 DAOQMB010000000
VERSION     DAOQMB010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_4183_cov_16.990552"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLY05_07245"
     CDS             complement(<1..509)
                     /locus_tag="PLY05_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HNC03135.1"
                     /translation="MFAPSFVFAETAPYITTMDKISAGDTAWMLTSAALVLLMTIPGL
                     ALFYAGMVRKKNVLSTALQSFAICAVITVVWVVIGYSLAFTPSSSPYIGTFDRLFLDG
                     MNFDKIQGTLSVSHIAPTIPESVFVVFQLTFAIITPALITGAFAERIRFGALIIFMAV
                     WSVLVYAPVA"
     gene            complement(801..1865)
                     /gene="hemE"
                     /locus_tag="PLY05_07250"
     CDS             complement(801..1865)
                     /gene="hemE"
                     /locus_tag="PLY05_07250"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HNC03136.1"
                     /translation="MSPLKNDRFLRALLRQPVDVTPVWMMRQAGRYLPEYRATRQQAG
                     DFMALCKNPQLACEVTLQPLERYPLDAAILFSDILTVPDAMGLGLYFETGEGPRFKKT
                     VKTEADVVALPIPNAEKDLDYVINAVKTIRGALNGRVPLIGFSGSPWTLATYMIEGGS
                     SKDFRAAKNMMYQQPQVLHMLLDKLAVSIIDYLNAQIRAGAQAVQIFDSWGGALSAAA
                     YQEFSLAYMKKIIDGLIKEHDGRKVPVILFTKGGGLWLESMATTGADALGLDWTIDIG
                     AARQRVGSKVALQGNMDPSVLYGSPQRIRDEVAHILAGFGHGEGHVMNLGHGIDQHTS
                     PEHAGAFINAVHELSAKYHQ"
     gene            complement(1917..>4183)
                     /locus_tag="PLY05_07255"
     CDS             complement(1917..>4183)
                     /locus_tag="PLY05_07255"
                     /inference="COORDINATES: protein motif:HMM:NF020921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HNC03137.1"
                     /translation="ERQGLCSQEQALRLFAAADYLCNMAMWLTVHMTYAKNVYLDGRE
                     LQASDFKKIPEGHTGGALNIVPAYVGYLLANALTGKTRAWLMGQGHCVAAIDAVNILL
                     GNTEPEQAARYPLSDAGLSQLAQDFYSYQIGADGHPAVPLGSHVNPYTAGGIIEGGYL
                     GFAELQYVHMPLPKQELVAFLSDGAFEEQRGSDWVPRFWRGEDTGLVMPIMIANGRRI
                     DQRTTMSQSGGVYWFKEHLKLNGFDPIEINGRDPAAFAWAIISMAQNLQQQHQDIQQG
                     KAHYPVYLPYAIAETVKGFGFVNAGTNAAHNLPIAQSPALHEAARVLFNQGCAPLYVP
                     SKQLIWAITMLNNHSFANRPLEKDHCLRKLQVSDLVAPPIKYLSLGEQLAPMVAIDKY
                     FSDLVTANPELRVRVANPDEMRSNRMNLTLDRIKHRVLDVELDEAEAVQGGVITALNE
                     EAVVSAVLANKQGLNIAVSYEAFAVKMLGAMRQEAIFARHLAELGRDLNWHSVPIVVS
                     SHTWENGKNEQSHQDPTLSEAWLGEMSDVAPVYFPCDANTAVAQLAALYQQKGRIATV
                     VAAKSVLPVWCDATQAQLAAQQGAITLRHDHQADVQLLAIGAYQLQEIERAAKRLRAK
                     GVGVSVVAIVEPARFRIARDAREAAYVWDDATVSNIVPEVQKRLFVCHGRPEVMTGVL
                     RRLDTGYQHSRFVGYHNRGGTLDVFGMLYANKITWAHIIFYVAELLAEDAQLWLSAEE
                     YLAVQGKGELECLR"
BASE COUNT         1281 a         1019 c          767 g         1116 t
ORIGIN      
        1 gcaacaggcg cataaaccaa aactgaccat actgccataa aaataatcaa cgccccaaaa
       61 cgaatacgct cagcaaatgc gcctgtaatt aatgctggag taataatggc aaacgttaat
      121 tgaaaaacaa caaatacaga ttcggggata gtcggtgcaa tgtgactaac actcaatgtt
      181 ccttgtattt tgtcaaaatt cataccatct aaaaataaac gatcaaatgt gccaatataa
      241 ggactagaac ttggtgtaaa agctaaacta taacctatca ccacccacac gacggtaatc
      301 accgcacaaa tagcaaaact ttgtaatgcg gtacttaata catttttttt acgcaccatg
      361 cctgcataaa ataaagccaa accaggtatg gtcatcaaca acactaaagc agcagatgtg
      421 agcatccacg cggtatcacc tgcgctaatc ttgtccatcg ttgtaatata aggtgctgtt
      481 tcagcaaata cgaaggatgg cgcaaacatg attgttgcaa ataataaata acacaacaga
      541 tcaagcggcc tattcatgac atacgctcct catttatttc taaatagggg gaatgcaaaa
      601 cttaggccaa ttaattaata gaatcaatgc actacaaaag catatcttgc gctttattag
      661 tgcattgata attagctgat gtttattata tttatgatga tctatttact tttcagtgca
      721 tattcagggc agaaatgcac taaagttgac taatctacga ctatttagcc ttgcactaaa
      781 tagtcgaaaa tagtgcaagc ttattgatga tacttcgcac ttagctcatg aaccgcatta
      841 ataaatgccc ctgcatgttc aggtgacgta tgctgatcaa taccatgccc taaattcatc
      901 acatgacctt caccatgacc aaagcccgct aagatatgag cgacttcatc acgaatgcgt
      961 tgtgggctgc catataacac tgaaggatcc atatttcctt gtaaagcgac tttgctgcca
     1021 acacgctgac gggcagcacc aatatctatt gtccagtcta agcccaaagc atccgcacct
     1081 gttgttgcca ttgactcaag ccacaaaccg ccacctttag taaacaaaat gacgggcact
     1141 ttgcgaccat catgctcttt gattaaacca tcaatgattt ttttcatata agctaaagaa
     1201 aattcttggt aagcggctgc tgataatgcc ccaccccaac tatcaaaaat ttgtacagct
     1261 tgtgcacctg cacgaatttg cgcatttaaa taatcaataa ttgatacggc taatttatct
     1321 aacaacatat gcaacacttg tggttgttga tacatcatgt tttttgcagc acgaaaatct
     1381 ttactcgaac caccttcaat catataggtg gctaatgtcc acggactgcc cgaaaaacca
     1441 attaacggca cacgaccatt gagcgcgcca cgaatggttt tgacggcatt aatgacatag
     1501 tctaaatctt tttcagcatt cggaatgggc aacgccacaa catccgcttc ggttttaact
     1561 gtctttttaa aacgagggcc ttcgcctgtt tcaaaataca agcctaatcc cattgcatca
     1621 ggcacagtca aaatatccga gaataaaatc gctgcatcca acggataacg ctctaaaggc
     1681 tgtaaggtga cttcacacgc caattgtgga tttttacaca aggccataaa gtcgcctgct
     1741 tgttggcgag tggcacgata ttctggcaaa taacgtcctg cttgacgcat catccagaca
     1801 ggggtgacat caacaggttg acgtaataaa gcacgtaaaa aacgatcatt ttttaaaggg
     1861 gacataaaag tttggctcta ggctaaataa attttaaaaa ttatctcaca ttctgtctat
     1921 cttaaacact ccaactctcc cttaccttgt actgccaaat actcttctgc cgatagccac
     1981 aattgagcat cttcagccaa caattcagca acgtaaaaaa taatgtgtgc ccacgtaatt
     2041 ttgttggcat acaacatccc aaacacatct aatgtaccac cgcgattatg ataaccaaca
     2101 aaacgactat gttgataacc tgtatccaaa cgtcttaata cacctgtcat cacttcaggg
     2161 cgaccatgac agacaaataa gcgtttttgt acctctggca caatgttact cacggttgca
     2221 tcatcccaca cataagccgc ttcacgtgca tctctagcga ttctaaaacg ggctggctca
     2281 acaatcgcca ccaccgacac tccgacacct tttgcacgta agcgtttggc agctcgttca
     2341 atttcttgca actgatacgc accaatcgcc aataattgca catcggcttg atgatcatgg
     2401 cgtaaagtga ttgcgccttg ttgtgccgct aattgtgctt gggtggcatc acaccaaaca
     2461 ggcaacacac tcttagccgc caccactgtg gcaatacggc ctttttgttg atacaaggcc
     2521 gccagttgcg caacagcggt attggcatca caaggaaaat agacaggcgc aacatcactc
     2581 atttcaccta gccaagcttc acttaaagta gggtcttgat gggattgttc gtttttgccg
     2641 ttttcccacg tatgagaact gaccacaatc ggtacactat gccagttaag gtctcggcca
     2701 agctcagcta aatgacgggc aaaaattgct tcttgacgca ttgcgcccag catttttacc
     2761 gcaaaggcct cataactaac ggcaatattt aaaccttgtt tattggctaa cactgctgaa
     2821 acgacggcct cttcatttaa ggcggtaatc acgccacctt gcaccgcttc ggcttcatcc
     2881 aactccacat ctaacacccg atgtttaata cgatctaggg ttaaattcat gcgattacta
     2941 cgcatttcat caggattagc gacccgtact cttaattcag gattagccgt caccaaatca
     3001 ctaaaatatt tatcaatcgc caccattggc gcaagttgtt cgcccaagga taaatactta
     3061 atcggagggg caactaaatc gctcacttgt aactttcgta aacagtggtc tttttctaaa
     3121 ggacgattag caaaactatg gttattgagc atggtaattg cccaaattaa ttgtttgctt
     3181 ggcacatata acggcgcgca accttggtta aataaaacac gtgccgcctc gtgtaaggct
     3241 ggactttgag caatcggtaa attgtgtgcg gcattagtgc ctgcattaac aaagccaaag
     3301 cctttcacgg tttcagcaat cgcataaggc aaatagacag gataatgcgc tttaccttgc
     3361 tgaatatctt gatgctgttg ctgtaaattt tgtgccatgc taataatcgc ccacgcaaac
     3421 gccgcagggt cacgaccatt aatttcgata ggatcaaagc cattcagttt aagatgctct
     3481 ttaaaccaat agacaccgcc cgattgtgac atggtggtac gttggtcaat acgacggcca
     3541 ttggcaatca taatcggcat aactaagccc gtatcttcac cacgccaaaa acgtggcacc
     3601 caatcactac cgcgttgctc ttcaaaagcc ccatcactca aaaacgcaac taactcttgt
     3661 ttgggtaaag gcatatgcac atattgcaat tcggcaaaac ctaaataacc gccctcaata
     3721 atgccacctg cggtataggg attaacatga ctgcctaatg gcacagcagg atgaccatct
     3781 gcaccgattt gatagctata aaaatcttgg gctaattggc ttaaaccagc atcgctcaat
     3841 ggataacgtg cggcttgttc aggctcggta ttgcccaata aaatattgac tgcatcaatc
     3901 gccgccacac aatgcccttg ccccatcaac catgcgcgcg ttttacctgt taaggcatta
     3961 gccaacaaat aaccaacata cgcaggcaca atatttaatg ccccgcctgt atgtccttct
     4021 gggatttttt taaaatcact ggcttgtaat tctcggccat ctaaatagac atttttagca
     4081 taagtcatgt gtacggttaa ccacatggcc atattgcaaa gataatcagc cgctgcaaat
     4141 aagcgtaatg cttgctcttg gctgcataaa ccttgtcgtt cta
//
LOCUS       DAOQMB010000194         4174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10463_length_4174_cov_17.534839, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000194 DAOQMB010000000
VERSION     DAOQMB010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10463_length_4174_cov_17.534839"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PLY05_07260"
     CDS             complement(<1..371)
                     /locus_tag="PLY05_07260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HNC03138.1"
                     /translation="MNLTFETFLPLLPVGILGATTVVVMLAIAFLRHHWWNATITVVG
                     LNLALASVIALVWQGTAPTAITPLLVVDNFAYFYMALILVAALACSTLTHAFIDSYKN
                     NREEMYLLLLIATAGALVLVC"
     gene            complement(379..1944)
                     /gene="nuoM"
                     /locus_tag="PLY05_07265"
     CDS             complement(379..1944)
                     /gene="nuoM"
                     /locus_tag="PLY05_07265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HNC03139.1"
                     /translation="MILSALILIPFIGGFLCWVGEKLGLGVPRWIALISMAAVLGLGC
                     QLWMQGDYSLANATVGTAPHWQLEERYTWIPHLGVSIHLALDGLSLLMVMLTGLLGIM
                     AIGCSWTEIQRHVGFFHLNLLWSLGGVIGVFISLDMFLFFFFWEMMLVPVYFLIALWG
                     HAGSDGKSRIYAANKFFIFTQAGGLIMLVGILALVLVNYQATGVLSFDYDALLGAHQY
                     MSPNLEMALMLTFFVAFAVKLPIVPVHSWLPDAHAQAPTAGSVDLAGILLKTAAYGLL
                     RFGLPFFPNASAEFAPIAMWLGIFGVFYGAFLAFAQTDIKRLIAYTSISHMGFVLIGI
                     YSGNLLTLQGLVIQMLAHGICAGGLFILSGQLYERIHTRDLREMGGLWGRFSYLPALL
                     MVFAAALLGIPGTANFIGEFLILLGAFKVVPLITIIATASLILAGVYSLIMIHRALFG
                     QAKSDEKLPDLSGRELALVVSLVGILVFLGLYPQPVLNVSQASMARIEQIYAPKAITV
                     PAVINTPAPTLAP"
     gene            complement(1954..3819)
                     /gene="nuoL"
                     /locus_tag="PLY05_07270"
     CDS             complement(1954..3819)
                     /gene="nuoL"
                     /locus_tag="PLY05_07270"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004794316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HNC03140.1"
                     /translation="MNLLYLTFVFPLVGFLLLASFRQRLSENVAAVIGVGSMGLSALT
                     ALYVGMDFLQNVPQGGSYVQTLWTWMQIGDFNASFGLHLDGLSLTMLGVVTGVGFFIH
                     LFASWYMRGENGFERFFSYMNLFVASMLLLVLGDNLVLLYLGWEGVGVCSYLLIGFYY
                     FDKANGEAAIKAFTVTRVGDVFMAIGLFILFKELGTLDIQQIVKLAPTHWQANDPMLV
                     FATLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFL
                     MTPEVLHLVGVVGAVTLVLAGFSALVQTDIKRVLAYSTMSQIGYMFLALGVGAWHAAI
                     FHLMTHAFFKALLFLSAGAVIIACHHEQNIFKMGGLYKKIPFVYACFLIGGGALAAFP
                     FITVGFYSKDAILLQAYASGNTDLFWAGLVGALFTSIYTFRLIYIVFHGKENGHAHEI
                     HGVSYWLPLGVLLVLSTGVGAFIHQPLEGVLPAFASPISEETKHFVEILAIGTGLVGL
                     AIGAALFMGERRVPTALANSAVGNALSRLWMNGWGFDWLYNWLFVKPYLLIAHLNRHD
                     GIDKAIGLIPRLARLGHSAVSTSENGRLRWYTASVLLGAVLVLGMVLYLQLNLRG"
     gene            complement(3816..4124)
                     /gene="nuoK"
                     /locus_tag="PLY05_07275"
     CDS             complement(3816..4124)
                     /gene="nuoK"
                     /locus_tag="PLY05_07275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HNC03141.1"
                     /translation="MIAIPMEHGLALAATLFVLGLVGVIVRRNILFMLMCLEVMMNAA
                     ALAFVVAGSRWAQPDGQVMFILILTLAAAEAAIGLAIVLQFYRRHHSLDIDVASEMRG
                     "
BASE COUNT         1494 a          998 c          772 g          910 t
ORIGIN      
        1 ctacaaacca ataccaaagc acctgcggtt gcaatcaaca acaacaaata catttcttca
       61 cggttgtttt tgtaactatc aataaaagca tgggttaagg tggaacaagc caatgctgcc
      121 accaaaatca aagccatata aaaataagca aaattatcaa cgaccaataa cggcgtaatg
      181 gctgtcggtg ctgtaccttg ccaaactaaa gcaatcactg aagccaaggc aaggtttaaa
      241 cccaccactg tgatcgttgc attccaccaa tgatgacgca agaaggcaat tgccaacatc
      301 accacaaccg ttgttgcacc caaaatgcca actggcaaca gaggtaaaaa agtctcaaaa
      361 gtcagattca taataacctc agggagccaa tgtgggtgct ggggtattga ttacagcagg
      421 cacagtgatg gcttttggag cataaatttg ctcaattcgt gccattgatg cttgcgaaac
      481 atttaataca ggttgtggat ataaacctaa aaacaccaag atacccacta aactgaccac
      541 taatgccaat tcacgacctg ataaatcagg caatttttcg tcagattttg cctgaccaaa
      601 taaggcacgg tggatcataa ttaaggagta aacacctgcc aaaattaaac tagcagtggc
      661 aataatggtg attaacggca caaccttaaa tgcacccaac aaaatcaaga attcaccaat
      721 aaagtttgct gtaccaggaa tccctaataa agcggcggca aataccatta ataacgcagg
      781 taaatagcta aaacgtcccc acaaaccacc catttcacgc aagtcacggg tgtggatacg
      841 ctcatacaat tggccagata aaataaacaa accacctgca caaataccat gtgccaacat
      901 ctgaatcact aaaccttgta aggttaataa gttaccagag tagataccaa tcaacacaaa
      961 acccatgtgg gaaatactgg tataagcaat taaacgttta atatcggttt gggcaaaagc
     1021 caagaatgca ccataaaaca cgccaaaaat acccaaccac atggcaattg gcgcaaattc
     1081 agcagaggca ttcgggaaga atggcaaacc aaaacgcaat aaaccataag ccgctgtttt
     1141 taacaaaata cctgctaagt caacactacc tgcggtaggg gcttgggcat gggcatctgg
     1201 caaccaagag tgaactggca caatcggtaa cttaacggca aaagcgacaa agaacgttaa
     1261 cattaaggcc atttctaaat taggtgacat gtattgatgt gcacctaata aggcatcgta
     1321 atcaaaactt aatacgcctg ttgcttggta attgactaat actaaagcca aaatacccac
     1381 caacataatc aagccgccag cttgggtaaa aataaagaac ttattagctg cataaatacg
     1441 gcttttacca tcagagcctg catgacccca taaggcaatt aagaaataaa caggaactaa
     1501 catcatctcc caaaagaaga agaataagaa catgtctaaa gagatgaata cgccaataac
     1561 accacccaaa ctccataata agttaaggtg gaagaagcca acatgacgtt gaatttctgt
     1621 ccaagaacag ccaatggcca taatacccaa taagcctgtt aacatgacca tgagtaaaga
     1681 caagccatct aaggccaaat ggatactcac acctaagtga ggaatccatg tataacgctc
     1741 ttctaattgc caatgaggcg ctgttccaac cgttgcattt gctaaagagt aatcaccctg
     1801 catccacaac tgacaaccta aacccaacac ggctgccata ctaatcaagg caatccagcg
     1861 tggaacaccg aggccaagct tttcgccgac ccaacacaag aaaccaccaa taaaggggat
     1921 taaaatcagt gctgaaagaa tcatggtctt gacttagcct cttagattta attgtagata
     1981 caacaccatg cctagcacta aaactgcacc aagcaataca ctggctgtgt accaacgtaa
     2041 acggccattt tcggatgtgc tgacggcact atgtcctaaa cgggcaagac ggggaatcaa
     2101 accaatggct ttatcaatac cgtcatgacg atttaaatgg gcaatcaata aataaggctt
     2161 aacgaataac cagttatata accagtcaaa accccaacca ttcatccaca aacgagacaa
     2221 ggcattaccc actgcactat ttgctaaagc agttggaaca cgacgttcac ccataaataa
     2281 cgcagcacca atcgccaagc ctactaaacc tgtaccaatg gctaagattt caacaaaatg
     2341 tttggtttct tcgctaattg gactggcaaa agctggcaat acaccttcta aaggttggtg
     2401 aataaatgca cctacgccag tagataacac taataacacc cctaaaggta accagtaaga
     2461 aacgccatga atttcatggg catgaccatt ttctttgccg tggaacacaa tataaattaa
     2521 gcggaaggta taaatagagg taaacaaagc accgactaaa cctgcccaaa ataaatcggt
     2581 attaccactg gcgtaggctt gcaacaaaat cgcatcttta ctgtagaaac ctacagtaat
     2641 gaatgggaac gctgccaatg caccgccacc aatcaagaaa caggcataga caaaaggaat
     2701 ctttttgtac aaaccgccca ttttgaaaat gttttgctca tggtgacaag caataattac
     2761 cgcacctgcg gataagaaca ataaggcttt aaagaaggca tgagtcatta agtggaaaat
     2821 tgctgcatgc catgcaccca cacccaaagc taagaacata taaccaattt ggctcatggt
     2881 tgaataagcc aacacacgtt tgatgtcggt ttgtactaaa gcagagaaac ctgctaatac
     2941 taaggtgact gcacccacga cacccactaa gtgtaaaact tctggggtca ttaagaacaa
     3001 accatgggta cgtgcaatta aatacacacc agcggtaacc attgttgctg cgtggatcaa
     3061 tgcagaaaca ggtgttgggc cagccatcgc atccgccaac catgtttgta atggtaattg
     3121 tgctgattta cccaccgcac cacctaataa cattaaggta gcaaagacca acatcgggtc
     3181 attagcttgc caatgggttg gtgctaattt aacgatttgc tgaatgtcta aagtgcctag
     3241 ttctttaaat aaaataaata aaccaatggc cataaacaca tcacccacac gtgtgacggt
     3301 gaatgcttta atcgctgctt cgccattggc tttgtcaaaa tagtagaaac caattaataa
     3361 ataactacaa acgcctaccc cttcccaacc taggtacaac agcactaagt tgtcacctaa
     3421 caccaataac aacatactgg caacaaatag gttcatataa gagaagaaac gctcaaagcc
     3481 attttcacca cgcatatacc atgaggcaaa taagtgaatg aaaaagccaa cacccgttac
     3541 tacacccaac atggttaagg acaaaccgtc taaatgcaaa ccaaaactgg cgttaaagtc
     3601 gccaatttgc atccatgtcc acaaggtttg cacataagag ccgccttgtg gcacgttttg
     3661 taagaagtcc atgcccacat ataaagctgt caaagcggat aagcccatcg agccaacacc
     3721 aatcactgcc gcgacgtttt cagacaaacg ttggcggaat gaggctaaca acaaaaatcc
     3781 cactaagggg aaaacaaagg ttaaataaag taagttcatc cgcgcatctc acttgccaca
     3841 tcaatatcga gcgaatgatg gcgacgataa aactgcaaca caatggccaa gccaatggca
     3901 gcttcggcag cagctaaggt taaaattaag ataaacatga cttggccatc aggttgtgcc
     3961 caacgactac ctgcaacaac aaacgctaag gctgctgcat tcatcatcac ttctaaacac
     4021 atcaacataa agaggatatt acggcgcaca atcacgccaa ctaaccccag tacaaagagc
     4081 gtggccgcga gtgccaaacc gtgctccatg ggtattgcta tcatacctgt accgcctttt
     4141 tgcctaggcg cattgcgcca tttaatttga aatc
//
LOCUS       DAOQMB010000195         4153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10547_length_4153_cov_14.408980, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000195 DAOQMB010000000
VERSION     DAOQMB010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10547_length_4153_cov_14.408980"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLY05_07280"
     CDS             complement(<1..428)
                     /locus_tag="PLY05_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017402001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HNC03142.1"
                     /translation="MAIEIKAPQFPESVADGTVAAWHKKVGDKVNRDEVLADIETDKV
                     VLEVVAPADGVLAQIVKAEGESVLSLETLAIFETGTAGLTLPASSAPVAEPAPTLAAT
                     VAASASADLAPAVRKAVAENQLDAGQISGTGRGGRITKEDV"
     gene            complement(451..3285)
                     /locus_tag="PLY05_07285"
     CDS             complement(451..3285)
                     /locus_tag="PLY05_07285"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HNC03143.1"
                     /translation="MHNGLMHQRSLSQLSAENAAYVEDMYEQFLTAPQSVSDEWRAYF
                     EQLPAYNGQQQDMPHKNVREQFLLLARNSTRVQPMQASSVSTEHEKRQVGVLQLIAAY
                     RNRGHQKAKLDPLKLMEREHVPDLELAAHGLSRADFDTVFQTGNLAIGKAEATLGEMV
                     NAMEAIYCGSIGAEYMHIVDTAEKRWIQSRLEGVRGNPNFAPEVKKHILERLTAAEGL
                     EKYLGNKYVGAKRFGLEGGESFIPLVDEIIQRAGGQGTKEIVIGMAHRGRLNLLVNSM
                     GKNPADLFDEFEGKTVNKYGSGDVKYHQGYSSNVMTAGGELHLALAFNPSHLEIVSPV
                     VEGSVRARQVRRDDIGGQKVIPIIVHGDAAFAGQGVVMETFQMSQTRGYTVGGTIHII
                     INNQVGFTTNKREDARSTEYCTDVAKMVQAPIFHVNGDDPEAVVFITQLALDFRMEFK
                     KDVVIDLICYRRRGHNEADEPSATQPLMYQVISKLPSTRTLYADKLVSANVLTRAEAE
                     AMVEDYRQALENGQHVVKSLVREPNKALYVDWTPYLGHKVIDDWSTGISKEKIAELAS
                     KLVKLPDDFVVQRQVQKVLEDRTQMWTGNMELNWGAAEVMAYASLLDEDYLVRITGQD
                     VGRGTFSHRHAILHSQKDAKSYAPLKAIRPDQHRFAMYDSLLSEEAVLAFEYGYATTY
                     PKSLIIWEAQFGDFANGAQVVIDQFICSGETKWERICGLTMLLPHGFEGQGPEHSSAR
                     LERFLQLCAEHNMQVCTPTTPAQIFHLLRRQAVRPLRKPLIVMTPKSLLRHKLATSKL
                     DELTTGGFQTVLPEVEAINPQAVTHVILCGGKVYYDLLEKRRDLKRDDVAIIRVEQLY
                     PFPEEKLIETLQPYVNAQSVVWCQEEPMNQGAWYSTRHRFQRVVAAWKPELDISYTGR
                     PSSAAPACGSPYLHAKQQAALIAAAFKLEQ"
     gene            complement(4061..>4153)
                     /gene="sdhB"
                     /locus_tag="PLY05_07290"
     CDS             complement(4061..>4153)
                     /gene="sdhB"
                     /locus_tag="PLY05_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681505.1"
                     /note="part of four member succinate dehydrogenase enzyme
                     complex that forms a trimeric complex (trimer of
                     tetramers); SdhA/B are the catalytic subcomplex and can
                     exhibit succinate dehydrogenase activity in the absence of
                     SdhC/D which are the membrane components and form
                     cytochrome b556; SdhC binds ubiquinone; oxidizes succinate
                     to fumarate while reducing ubiquinone to ubiquinol; the
                     catalytic subunits are similar to fumarate reductase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HNC03144.1"
                     /translation="IMNCVSVCPKGLNPTKAIGHIRNMLLEQAS"
BASE COUNT         1227 a          934 c          787 g         1205 t
ORIGIN      
        1 acatcttctt tagtgatgcg accaccacga cctgtaccag aaatttgacc tgcatctaac
       61 tgattttcag ccactgcttt gcgaacagct ggtgctaaat ctgcacttgc actcgccgca
      121 actgttgctg ctaaagtagg tgctggctca gcaactggtg cggagctagc gggcaaggtt
      181 aaacctgctg tacctgtttc aaaaatagct aaagtttcta aacttaagac agactcgcct
      241 tctgctttaa cgatttgtgc caaaacacca tcagcagggg caactacttc taacacaact
      301 ttatcagttt cgatatctgc taaaacttca tcacgattaa ctttgtcgcc cacttttttg
      361 tgccaagcag caactgtgcc atcagcaacg gactctggga actggggagc tttaatttcg
      421 atagccatac gagtaatcct tctttattaa ttactgttca agtttaaacg cagcggcaat
      481 aagtgctgct tgctgtttgg catgaagata aggtgagcca caggctggtg cagcagagga
      541 tggacgacct gtgtaactaa tgtctaattc aggtttccat gctgcaacaa cacgttggaa
      601 gcgatgacga gtgctatacc atgccccttg attcattggc tcttcttgac accaaaccac
      661 actttgcgcg ttgacataag gttgtaaggt ttcaatcaat ttttcttcag ggaatggata
      721 caattgttca acacgaataa tggcgacatc atcacgtttt aaatcacggc gtttttctaa
      781 caagtcataa taaaccttgc caccacataa aatgacatgg gtcacggctt gcggattaat
      841 cgcttccact tctggcaata ccgtttggaa accacctgtg gttaattcat ccaatttgga
      901 agttgctaac ttgtgacgca acaaactctt tggagtcatg acgatcaatg gtttacgcaa
      961 tgggcgtact gcttgacgac gtaacaaatg gaaaatttgt gcgggcgttg ttggcgtaca
     1021 tacttgcata ttatgttcag cacacaactg caagaaacgc tctaaacgag cagatgaatg
     1081 ctcagggcct tgaccttcaa aaccatgtgg caacaacatg gttaagccac aaatacgctc
     1141 ccacttggtt tcaccactac aaataaactg gtcgatgacg acttgtgcgc cattagcaaa
     1201 gtcaccaaat tgcgcttccc aaataatcaa tgattttgga taagtagtgg cataaccata
     1261 ttcaaaggct aaaactgctt cttcggacaa cagcgagtca tacatggcaa aacgatgctg
     1321 atcaggacga attgctttta gaggtgcgta agatttggca tctttttgac tatgaagaat
     1381 agcatgtcga tgtgagaatg taccacgacc aacgtcttgg ccagtaatac ggactaagta
     1441 atcttcatct aacaaggacg cataagccat cacttcggct gcaccccagt ttaattccat
     1501 attacccgtc cacatttgtg tacggtcctc tagtactttt tgaacctgac gttgcacaac
     1561 aaagtcatca ggtaatttga ctaatttgct agctaattca gcaattttct ctttactgat
     1621 acctgttgac caatcatcaa tcactttgtg acctaaataa ggcgtccaat caacatataa
     1681 cgctttatta ggctcacgca ccaaggattt aacaacgtgt tggccatttt ctaaagcttg
     1741 gcgataatct tcgaccatcg cttcggcttc ggcacgcgtc aatacattag cagacactaa
     1801 cttatcggca tacaaagtgc gtgtgctggg caatttgcta atgacttgat acatcaaagg
     1861 ctgagtcgct gatggctcat cggcttcatt gtgaccacga cgacgataac aaatcaaatc
     1921 aataacaacg tcttttttga actccatgcg gaaatctaaa gccaattgcg taataaacac
     1981 gactgcttct ggatcatcac cattgacatg aaaaatgggt gcttgcacca ttttggcgac
     2041 atcggtacag tattctgtcg aacgagcatc ttcacgttta ttggtggtaa accctacttg
     2101 gttattgatg atgatatgaa ttgtgccacc cacagtataa ccacgagttt gcgacatttg
     2161 gaaagtttcc atgaccacgc cctgacctgc aaatgcagca tcaccgtgaa caataatagg
     2221 aataactttt tgaccaccaa tatcatctcg gcgtacttga cgcgcacgca ccgaaccttc
     2281 aacaacaggc gaaacaattt ctaaatgtga agggttaaaa gctaatgcta aatgcagttc
     2341 accaccagcc gtcatcacat ttgatgaata accttggtga tacttcacat cacctgaacc
     2401 gtatttattg acggttttac cttcaaattc atcaaataga tcggctgggt ttttacccat
     2461 gctatttact aacaaattta aacggccacg gtgcgccata ccaataacaa tttctttggt
     2521 accctgtcca cctgcacgtt gaataatttc atccactaaa ggaataaaac tttcaccgcc
     2581 ttctaaaccg aagcgtttag caccgacata tttattacct aaatattttt ctaaaccttc
     2641 agcagcggtt aaacgctcta aaatatgttt tttaacttcg ggagcaaaat taggattacc
     2701 acgcacccct tccaaacggc tttgaatcca acgtttttcg gctgtatcaa caatgtgcat
     2761 atactccgca ccaatggagc cacaataaat agcctccata gcgttgacca tttcgcccaa
     2821 agttgcttcg gctttgccaa tggctaaatt acctgtttgg aatacagtat caaagtcagc
     2881 acgagacaag ccatgtgctg ccaactctaa atcgggaaca tgttcacgtt ccattaattt
     2941 gagtgggtca agctttgctt tttgatgacc acggttacgg taagcagcaa ttaactgtaa
     3001 gacacccacc tgacgctttt cgtgttcggt actcacacta ctggcttgca tcggttggac
     3061 acgggtactg ttgcgggcta ataataaaaa ctgttcacgg acatttttat ggggcatatc
     3121 ttgttgttga ccgttgtaag cgggtaactg ttcaaaataa gcacgccatt cgtcagagac
     3181 agattgaggc gcagttaaaa actgctcata catgtcttct acataggctg cattttcagc
     3241 cgaaagctgg gacaagcttc gctggtgcat tagaccattg tgcatggtaa atctctctcg
     3301 caacatatgc taaatacctt agttgttttt agcagtgtta cttttccttt aggattaaaa
     3361 aatcctgtgt tttatcatac gatacgcaaa tgactggata agccatttac agccaaggtt
     3421 gaatcgtgtg tcacacgctc aactacgtaa gtagggtttt atgttataca tcaacacgtt
     3481 aagatttaca cgtgaaaatc ttcaactaaa ccctcaaatt gttaacttaa ccgtgaatta
     3541 cttcactttg tttagcatga cgctattaaa cttgaccatc aaacaacgtc tgcatcaatt
     3601 taagatatag cctaaagcac aatgagcata agatattgat tagatgtgac agctttatgc
     3661 cacaagctct ttacgagcta tgctggtgca tttttatcaa ttctacattt ctatgtactt
     3721 ttatagtgca tctcagttaa gtgttgcacc gactaccagc attggtagag actaagcaaa
     3781 aaaactgagg aaataaatag cttttatcaa aaaagattta gtgtttttaa tattttgctc
     3841 aataaaacac ataacaagct tgattgaccg actttgtcag ctacttcaat aacaacagca
     3901 tcaacgctga cccgcccacg ttagcaggat ttgaaggttt attagcaaga taacttgctg
     3961 ttctttgaaa tattttgcaa tacatatgcc aaattgtttc aaaaacaaaa aaactcatca
     4021 ggtgatgagc ttttttgctg atatttcaaa caatcaagac ttaacttgct tgttctaata
     4081 acatattgcg gatatggcca atagccttag ttggatttaa gccttttgga catacgctaa
     4141 cgcagttcat aat
//
LOCUS       DAOQMB010000196         4145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10588_length_4145_cov_14.132274, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000196 DAOQMB010000000
VERSION     DAOQMB010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4145
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10588_length_4145_cov_14.132274"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..282
                     /locus_tag="PLY05_07295"
     CDS             1..282
                     /locus_tag="PLY05_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016138215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HNC03145.1"
                     /translation="MCSIYKSSKKDEMYLYVRKAEGFARVPEALLTMFGTPKLFGTLL
                     LTPSKKLARADVHLVLADLAEKGFYLQMPPPREDYMLDLFRPDESKYQQ"
     gene            295..609
                     /locus_tag="PLY05_07300"
     CDS             295..609
                     /locus_tag="PLY05_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03146.1"
                     /translation="MLGYVVAWVVYVASALAIWWVYQRGIARLLPETIRSVVAVLMMC
                     VLLTPWPIDQETWLLAPAIIATIFNVFSGFGIAALKSLSPILLVSTIACFVAWLKTRK
                     SY"
     gene            619..1458
                     /locus_tag="PLY05_07305"
     CDS             619..1458
                     /locus_tag="PLY05_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC03147.1"
                     /translation="MQFTGTEAYVATEDLKLAVNAAIKLQKPLLIKGEPGTGKTLLAE
                     QVAQSLGLKLIQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDNRVFDIKNYIKQGKLWE
                     AFASDERVVLLIDEIDKADIEFPNDLLTELDKMEFFVYETGETIKAKYRPIVIITSNN
                     EKDLPDAFLRRCFFHYITFPDAETMKRIVAVHYPNIQQTLVKEALDIFFDLRKVAGLK
                     KRPSTSELIDWLKLLMSDDLPEDILRNRDTRTAIPPLHGALLKNEQDVHLLERLAFMS
                     RRG"
     gene            complement(1554..1964)
                     /locus_tag="PLY05_07310"
     CDS             complement(1554..1964)
                     /locus_tag="PLY05_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03148.1"
                     /translation="MKVVDTNILARFFIDDPDDEQACLQRPIATIILSESVYVPITVI
                     LEFEWVMRGFYKLDRAEILHIYHVLLSFAHVQIEDHTIVLTALSYYQQGLDFADSLHA
                     ARSQAYDSFITFDVKFFKKAQRITTLNVELAKSV"
     gene            complement(1961..2203)
                     /locus_tag="PLY05_07315"
     CDS             complement(1961..2203)
                     /locus_tag="PLY05_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03149.1"
                     /translation="MMTALIINLPDELVQKAEQIGILQTALLSEYVSQFLEEKIRQQS
                     RMTDVSAFSGLVKAKSTGKKRSLDDFDAASFVESSQ"
     assembly_gap    2478..2577
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2578..3291
                     /gene="pyrD"
                     /locus_tag="PLY05_07320"
     CDS             <2578..3291
                     /gene="pyrD"
                     /locus_tag="PLY05_07320"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HNC03150.1"
                     /translation="FNNLGVDHLVNQVKKAKYQGVLGINIGKNFDTPVENAADDYVHC
                     LEKVYDLASYVTVNISSPNTQGLRSLQTGSAFSDLLSQLKETQARLATQYGHYVPLVV
                     KIAPDLQDEEIEYMADQLVQYKIDGVIATNTTITRPGIESLNLASETGGLSGKPLKAL
                     SNYTLSRLCTALEGRIPVMGVGGITDGMSAADKITTGASLVQLYTGFIYQGPQLIEDA
                     VAAIKALEQLQSAAQEDVV"
     gene            3293..3823
                     /locus_tag="PLY05_07325"
     CDS             3293..3823
                     /locus_tag="PLY05_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HNC03151.1"
                     /translation="MPLADILILGLLAISIWKGFSRGIIREGLALAGWVVGLIVAING
                     YQSFAEVLAPFISTPSLQKAVAFLTLCLSMVILSTLIALVLAELLKALALRPVDKLAG
                     AGFGLARGLLIVLLAIGLMAPFVQDDAWWQEASMPKALLPYVPVAHLVTDEIKKQVHH
                     LPQTKIQIPTRQPSQQ"
     gene            3832..>4145
                     /locus_tag="PLY05_07330"
     CDS             3832..>4145
                     /locus_tag="PLY05_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNC03152.1"
                     /translation="MCGIVGIAGKSPVNQELYDALTMLQHRGQDAAGIVTAQDGRLFL
                     RKDNGMVRDVFHTRHMLRLVGNFGIGHVRYPTAGSSSSAEAQPFYVNSPYGITLAHNG
                     NLT"
BASE COUNT         1171 a          748 c          841 g         1285 t
ORIGIN      
        1 atttgtagta tttacaaaag ttcaaaaaaa gatgaaatgt atttgtatgt acgtaaagca
       61 gaaggatttg ctcgagtgcc agaagcttta ttaaccatgt ttgggacacc caaattgttt
      121 ggtacgttgc tgttaacacc ttcaaaaaaa ctggcgcgtg ccgatgttca tttggtattg
      181 gccgatttgg ccgaaaaagg tttttattta caaatgcctc caccgcgtga agattatatg
      241 ctcgatttat tccgtcctga tgaaagtaaa tatcagcaat aaaggagtag tgctatgttg
      301 ggatatgtgg tggcttgggt ggtttatgta gcctcagcct tagccatatg gtgggtttat
      361 caacggggta ttgcacgttt attacccgaa acaatccgtt cagtagtagc tgtactcatg
      421 atgtgtgtat tattgacacc gtggcctata gatcaagaaa cttggttgct agctcctgcg
      481 attattgcca ccatttttaa tgttttttcg ggttttggta ttgctgcttt aaaaagttta
      541 tcaccgattt tattggtttc aaccattgcg tgttttgtgg cttggctaaa aacacgtaaa
      601 tcttattgag agcatgatat gcaatttact ggtacagagg cttatgtagc caccgaagac
      661 ttaaaattag ctgttaatgc agccattaaa ttacaaaagc cgttattaat taagggcgaa
      721 ccaggcactg gcaaaacctt attagcagag caagttgctc aatctttggg gttaaagctg
      781 attcaatggc acattaaatc aacaaccaaa gcacaacaag gcttgtatga gtacgatgct
      841 gtgtctcgtt tgcgtgattc gcagttaggt gataatcgcg tttttgatat taaaaactac
      901 attaaacaag gtaaattgtg ggaagcattt gccagtgatg agcgtgtggt gttgttgatt
      961 gatgaaatag acaaagcgga tattgagttt cccaatgact tgttaaccga actcgataaa
     1021 atggaatttt ttgtttacga aacaggcgaa accattaaag ccaaatatcg cccaattgtg
     1081 attattacct ctaacaacga aaaagattta cctgatgcct ttttacgccg ttgttttttt
     1141 cactatatca ctttccctga tgccgaaacc atgaaacgga ttgtggcagt gcattatccc
     1201 aatattcagc aaaccttagt caaagaagct ttagatattt tctttgattt gcgtaaagtc
     1261 gcaggtttaa aaaagcgtcc ttcaacctca gagttaattg attggttaaa attattaatg
     1321 tctgatgatt tacccgaaga tattttacgt aaccgtgata ctcgtaccgc gattccaccg
     1381 ttgcatggtg ctttactaaa aaatgagcaa gacgtgcatt tgttggagcg tttggcattt
     1441 atgagtcgga gagggtgaaa tcctcctccc caaccctcct ttgataaagg agggggcttc
     1501 atcgctgatg acgtactgtg cgttttaatg aggtgtatca gtgataatag ccattatact
     1561 gatttagcca gctcaacatt aagcgtcgtt attcgctgcg cttttttgaa aaatttaaca
     1621 tcaaaagtga taaaagagtc ataagcctgt gagcgtgcag catgtaaact atcagcaaaa
     1681 tctaaacctt gttgataata gcttaaagcc gttaaaacga tagtatgatc ttcaatttga
     1741 acatgggcaa aactgagtaa aacgtgataa atatgtaaaa tttcagcacg atctagcttg
     1801 tagaaaccac gcatcaccca ttcaaactct aaaatcacag tgattggaac ataaacagat
     1861 tctgataaga taattgttgc aattggacgt tgcaggcaag cttgttcatc atcaggatca
     1921 tcaataaaaa aacgagcaag tatgttggta tcaacgactt tcattgtgat gactcaacaa
     1981 agctagcagc atcaaaatcg tctaaactcc ttttttttcc tgtactttta gctttaacta
     2041 aacctgaaaa cgctgaaaca tctgtcatgc gactttgttg tcgaattttt tcttctaaaa
     2101 actggctaac atattcacta agtaaagctg tttgtaatat accgatttgt tcggcctttt
     2161 gtactaattc atcaggtaaa ttaatgatta aagcggtcat catatacctc gttatctttt
     2221 gtatgggctt aaaatcatag catatttggg gagatgcact gtcaaaatta ttgtttgtga
     2281 ttttttaagc aagagcgtat atcaactatg gtaacaccta tgtgaataat gcctatcaac
     2341 cataaaacgt gtataatccg cgccttgcta ggtttaaaag ggtttgttta ctgacaaacc
     2401 ctgaggtgcg ttatgtatta tcccttaatc cgttctttgt tatttcgttt tgatgcagaa
     2461 acatcccatc atgttgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
     2581 aataatttgg gtgttgacca tttagttaat caagtcaaaa aagccaaata tcagggtgta
     2641 ttagggatta atattggcaa aaactttgat acacccgtcg aaaatgcggc tgatgattat
     2701 gtgcattgtt tagaaaaagt ttacgatcta gcctcttatg tgacggtgaa tatttcttca
     2761 ccaaataccc aaggtttacg ctctttacaa actggttcag cgttctctga tttattaagc
     2821 caactcaaag aaactcaagc tcgcttggct acacaatatg gccattatgt gccattagta
     2881 gtaaaaattg cacccgattt acaagatgaa gaaattgagt acatggccga tcaattggtg
     2941 caatacaaaa ttgacggtgt gattgccacc aataccacca ttaccagacc aggtattgaa
     3001 agcttaaact tagccagtga aacgggtggt ttaagcggta aacctttaaa agctttatct
     3061 aattacacct tgtctcgttt gtgtactgct ttagagggac gtattcctgt gatgggagta
     3121 ggtggtatta ccgatggtat gtccgccgcc gacaaaatta ccacaggcgc aagtttggta
     3181 cagttatata caggctttat ttatcaaggc ccacaattga ttgaagacgc tgtcgctgcg
     3241 attaaagcct tagaacagtt acaatctgct gcacaagaag atgtagtcta agatgccatt
     3301 agccgatatt ttaattttgg gtttgcttgc tatttcgatt tggaaaggct ttagccgtgg
     3361 cattattcgt gaaggcttgg ctttagcagg gtgggttgtt ggcttaattg tggccattaa
     3421 tggctatcaa agctttgctg aggtattagc accttttatt agcacgccct ctttgcaaaa
     3481 agcggttgcc tttttaacct tgtgcttaag tatggttatt ttaagcactt taattgcgtt
     3541 agtattagct gagttgctta aagccttagc cttgcgtcct gttgataaat tagcaggtgc
     3601 aggattcggc ttggcacgcg gcttattaat tgtgttattg gccattggtc taatggcacc
     3661 gtttgttcag gacgatgctt ggtggcaaga agcaagtatg cccaaagcat tgttacctta
     3721 tgtacctgtt gcacatctcg ttactgatga gatcaaaaaa caggtgcatc atttacccca
     3781 aaccaaaatt caaattccta ctcgtcaacc tagccaacag tgaggtaaat tatgtgcggt
     3841 atcgtcggca tagctggtaa atcgcctgtt aatcaagaac tttatgacgc actgacgatg
     3901 cttcaacatc gtggccaaga tgcagcaggg attgtcaccg cacaagatgg acgtttattt
     3961 ttgcgtaaag ataacggcat ggtacgtgat gtttttcata cacgtcacat gttgcgttta
     4021 gtgggtaact ttggcattgg tcatgtgcgt tatccaaccg caggctcctc tagctctgcc
     4081 gaagctcaac cgttttatgt caattcacct tacggtatta ctttggcgca taatggtaat
     4141 ttaac
//
LOCUS       DAOQMB010000197         4134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10636_length_4134_cov_16.443981, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000197 DAOQMB010000000
VERSION     DAOQMB010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10636_length_4134_cov_16.443981"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>2600)
                     /locus_tag="PLY05_07335"
     CDS             complement(156..>2600)
                     /locus_tag="PLY05_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNC03153.1"
                     /translation="RFETFIYFSLAAIMPGLFLLMSYPSIVNSALMGVSGLFFFFVLL
                     AGRRLSDLSYQSLWLQLRNEDLIHFLENSKTSAESLNQQLTQEIQDRRQTESQLQEIN
                     LNLEQKVRERTLALSDINRALRNSQERLTLAIDASGIGLWDWNMTTNEIFHSNFEQLL
                     GYSKLEMKNLMGHMQEIIHPDDYPSVRHALVQHLRKKRLNYEVAYRIRHRSGYWVWVE
                     DNGRVVAWDEKGKPLRLIGTRRDITPEREIEAKLRLSASVFEHAAEGIYILDESFCFL
                     SVNPRFSQMLGFNHEELEGKNIIDYVVETSGHRQATYVNILSQLKTVGDWQGELTDYH
                     KNGTPIPLWLHITAVVNHDEHIVHYVGIVSDLSQRKEAEEKLKYLANYDRLTGLANRN
                     LFYDRLHSAISKGREQATGFAIIHIDLDRFRTLNDSLGHEVGDNLLKKVAERISHSAG
                     DANTVARLGSDEFALMLDQTTDHKVIESLCEKIIEQLRKPFKIAEHELMLGASIGVSI
                     FPEHGRELQILINNADLALQQAKRMGGNSTHFFNSNVKAISIEQVNLESALRKAIFRD
                     EFVVFYQPKLCLKSNRIIGTEALVRWQHPSLGLLAPGQFIPLAEEAGLISAIGEIVLD
                     KACRQTHAWHNLGFQITTSVNVVAQQLQRGNLLDIVDRVLLNTELDPLSLELELTESS
                     FMEDKETVRTTLENLRYRGIKIALDDFGTGYSSLSYLRDFPIDVLKIDQSFVFRIGQT
                     EKDDAIVKAIFAMGHSLGMDIVAEGVETIENLDFLKAQGCDMVQGYLISRPIPAEQLT
                     AMLNEEAHNTTLKNLA"
     assembly_gap    2601..2700
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2776..3732
                     /locus_tag="PLY05_07340"
     CDS             2776..3732
                     /locus_tag="PLY05_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HNC03154.1"
                     /translation="MKLQPLVLALGLGLGVATAHALDAGMPEYQKTAGISGNLSSVGS
                     DTLANLMTFWAEDFKKQYPNVNIQIQAAGSSTAPPALAQGTANFGPMSRAMKDNEIQD
                     FEKKHGYKPTAIPVAVDALAVFVHKDNPIKGLSMPMVDAIFSTTRKCGFEKAVNTWGD
                     AGLTGTWASRTLQLFGRNSVSGTYGYFKEHALCKGDFKSNVNEQPGSASVVQSISNSI
                     NGVGYSGIGYVTSGVKAVPIKNSDGEFIAATAKNALSGKYPLARYLYVYVNKHPKKPL
                     APLEAEFVKLVLSKQGQEVVEKDGYIALPASEVKKVRAKLGL"
BASE COUNT         1240 a          772 c          779 g         1243 t
ORIGIN      
        1 cttttgcacc tcctgcatta ggtcaaacca cattaaccaa tgagcaacaa gctgctttaa
       61 caacgattat tacaaccagt tggtaataaa aaagcccctt gtgtatttac acaaggggct
      121 ttttttagtt aacgtcaacc tctattttag cgatattaag ccaagttttt taatgtggtg
      181 ttgtgagctt cttcattaag catcgctgtt aattgctcag caggaatcgg cctactgatt
      241 aaatatcctt gcaccatgtc gcagccttgt gcctttaaaa agtctaaatt ttcaatagtt
      301 tctactcctt cagcgacaat atccatacct aggctatgtc ccattgcaaa aatagcttta
      361 acaatggcat cgtctttttc ggtttggcca attctaaaca caaaagattg atctattttg
      421 agaacatcaa tcggaaagtc acgtaaataa gataaagaag agtagcctgt accaaaatca
      481 tccaaagcga tttttatgcc tcgataacgc aaattctcta aagtagtgcg tactgtttct
      541 ttatcttcca taaaagaaga ttcggtgagc tctaattcta ggcttaaagg atcaagctcg
      601 gtatttaata aaacacgatc aacaatatca agtaaatttc ctcgttgcag ttgttgggcg
      661 acaacattta ccgaggttgt aatttgaaaa cctaaattat gccaagcatg ggtttgacga
      721 caagctttat ctaaaactat ttcaccaata gcagaaatga ggccagcttc ttctgctaaa
      781 ggaataaatt gaccaggtgc taacagtcct aaactcggat gttgccaacg cactaaagct
      841 tcagtgccaa taatacgatt gctttttaaa cacaatttgg gttgataaaa aactacaaat
      901 tcatctctaa aaatcgcttt gcgtaaagca ctttctaaat taacttgctc aatcgaaatc
      961 gcttttacat tagagttaaa gaagtgtgtg ctgttgccac ccatacgttt agcttgttgt
     1021 agtgcaagat cagcattatt aatcagaatt tgtagctcgc ggccatgttc tggaaaaata
     1081 ctgacaccaa ttgaggcacc aagcataagt tcgtgttcag caattttgaa gggtttacgt
     1141 agttgctcaa tgattttttc acataaactt tcaatcactt tatgatcggt ggtttggtct
     1201 aacattaagg caaactcatc actgcctaaa cgtgcgacag tgttggcatc acccgcacta
     1261 tgactaatac gctcagcaac ttttttaagt agattgtcac ctacttcatg acccaagctg
     1321 tcatttaagg ttctaaaacg gtctaaatca atatgaataa ttgcaaagcc tgtggcttgt
     1381 tcgcgtccct tgctaatggc actgtgtagg cgatcataaa ataagttacg attggctaag
     1441 cctgttagac gatcataatt ggctaaatat ttgagttttt cttctgcttc tttacgttgg
     1501 cttaaatcag aaacaatccc cacgtaatgc acaatatgtt catcatgatt gactacagca
     1561 gtaatgtgta accaaagggg aatcggtgtg ccgtttttat ggtagtcagt taattctcct
     1621 tgccaatcac ccacggtttt aagttggctt aagatattta cataggttgc ttggcgatga
     1681 ccagatgttt cgacaacata gtcaataata tttttgcctt ctaattcttc atgattaaag
     1741 ccgagcattt gactaaaacg aggattaacc gataaaaagc aaaatgattc gtctaagata
     1801 taaatacctt cggcagcatg ttcaaatacc gaagcggata accgtaattt tgcttcaatt
     1861 tcgcgctctg gcgtaatatc tcggcgtgta ccaattaaac gtaaaggttt gcctttttca
     1921 tcccaagcaa cgactcgacc attatcctca acccagaccc aataaccaga acgatgtcta
     1981 atgcgataag cgacttcata attgagtcgt tttttgcgta aatgttgaac taaggcatga
     2041 cgtactgaag gataatcatc aggatgaata atttcctgca tgtgtcccat aagatttttc
     2101 atttctaatt tggagtagcc taaaagctgt tcaaaattgg aatgaaaaat ttcattggtg
     2161 gtcatattcc aatcccacaa accaatgcca gaagcatcaa tagctaaagt cagtcgttct
     2221 tggctattgc gtagagcacg gttaatgtca gataaggcta aagttctttc gcgtactttt
     2281 tgttctagat taaggttaat ttcttgcagt tggctttcgg tttgtcgacg atcttgaatt
     2341 tcttgagtaa gttgttgatt taatgattcg gcagaagttt tagagttttc taaaaagtga
     2401 attaagtctt cattgcgcag ttgtaaccat aaagattgat aactgagatc acttaaacga
     2461 cgacccgcta acaagacaaa aaagaaaaat aaaccactca ctcccatcaa tgcagagtta
     2521 acaatagatg gatagctcat taacaaaaat aagccaggca tgatggctgc taatgaaaaa
     2581 taaataaagg tttcaaaacg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 gattgtcata atactgtcat aaatccttcg taatatgcca tcatcttaaa gaatcatact
     2761 cattaggagt ttcttatgaa gctgcaacct ttagttttag cattaggttt gggtttaggc
     2821 gtagcaacag cccatgcttt agatgctggt atgcctgaat atcaaaaaac ggctggtatt
     2881 tctggtaact tatccagtgt aggttctgac accttagcca atttaatgac attttgggca
     2941 gaagacttta aaaagcaata tcctaatgtc aacattcaaa ttcaagcagc aggttcttcg
     3001 actgcaccac ccgcattagc acaaggtact gccaactttg gtccaatgtc tcgtgcaatg
     3061 aaagacaacg aaattcaaga ttttgaaaag aaacatggtt ataagccaac tgctattcct
     3121 gtggctgtgg atgccttagc tgtatttgtt cataaagata accccatcaa aggcttatct
     3181 atgccaatgg ttgacgctat tttttcaaca acacgtaagt gtggttttga aaaagcggta
     3241 aacacatggg gtgatgcagg tttaactggc acttgggcaa gtcgtacctt acaattattt
     3301 ggtcgtaact ctgtttctgg tacgtatggt tattttaaag aacacgcttt gtgtaaaggt
     3361 gactttaaat ccaacgtcaa tgaacaacca ggttcggctt ctgtggtgca atccatttct
     3421 aattcgatta acggtgtcgg ttattcgggt attggttatg tcacctcggg tgtaaaagct
     3481 gttccaatta aaaacagcga tggcgaattt attgctgcaa ccgccaaaaa tgctttatca
     3541 ggcaaatatc ctttagcacg ttatttgtat gtgtatgtta acaaacaccc taaaaagcct
     3601 ttagcaccat tagaggctga atttgtgaaa ttagtattat ccaaacaggg acaggaagtg
     3661 gttgagaaag atggttacat cgctttacca gctagcgaag tgaagaaagt acgagcgaag
     3721 ctaggtttgt aatactgtat aataaaaagg ggctgacagc ccctttttgt ttttagccta
     3781 gatgtgagct taatggttgt gagtcaaaat tgctgcaatc ttaagcgatt taaggctgtt
     3841 atcattcgca tagtcctaat ctttagggct tacgcatttc accgaaatta gcgcgttttg
     3901 tgaacataaa atcgttgaaa acgattgaaa tgtgagcaaa taagcgataa ctgcgtaagc
     3961 cctaatattg tttaattaat tcgagagaag tatgagtaaa ccagagcgtg ttcgcctaga
     4021 tgcagcaaca atggctgccc tagaccgtcg ccgccgtatc aaagacaaaa tcgctaatgt
     4081 gacgattggt tttggcggtg tatttgtggt gttagccatt accttgattt ttgt
//
LOCUS       DAOQMB010000198         4109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10768_length_4109_cov_16.497040, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000198 DAOQMB010000000
VERSION     DAOQMB010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10768_length_4109_cov_16.497040"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..217
                     /locus_tag="PLY05_07345"
     tRNA            142..217
                     /locus_tag="PLY05_07345"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:175..177,aa:Trp,seq:cca)
     gene            245..631
                     /gene="secE"
                     /locus_tag="PLY05_07350"
     CDS             245..631
                     /gene="secE"
                     /locus_tag="PLY05_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNC03155.1"
                     /translation="MTTQAEDVTTNNSLNTVKWVVAIAILIAATVGNRYAPELLPQLS
                     SWVRIVALVVLAVGALAITFTTTQGQSFIKLLKEAQVEARRIVWPTKDETMQTTMIVC
                     AVVVVMSLLLWGIDTLFGWMISAVIG"
     gene            646..1182
                     /gene="nusG"
                     /locus_tag="PLY05_07355"
     CDS             646..1182
                     /gene="nusG"
                     /locus_tag="PLY05_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNC03156.1"
                     /translation="MSKRWYIVHAYSGFEKQVMRSLQERIKRAEMEHLFGEVLVPTEE
                     VVEIKNGVKRKSERKFFPGYVLVHMEMNDDSWHLVKDCPKVMGFIGGKADKPAPITDK
                     EADAILSRLRQSTDAPRPKTLFEPGEVVRVVDGPFADFNGAVEEVHYEKSRLRVAVMI
                     FGRATPVDLEFNQVEKVS"
     gene            1329..1757
                     /gene="rplK"
                     /locus_tag="PLY05_07360"
     CDS             1329..1757
                     /gene="rplK"
                     /locus_tag="PLY05_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNC03157.1"
                     /translation="MAKKIDAYVKLQIPAGKANPSPPIGPALGQRGVNIMAFCKEFNA
                     ATQKMEVGQPTPVVITVYSDKSFTFTMKSPPATFLLKKAAGIKSGSKRPNTQKVGKVT
                     RAQLEEIVNIKKADLTAADMDAAVRTLSGSARSIGIEVEG"
     gene            1762..2460
                     /gene="rplA"
                     /locus_tag="PLY05_07365"
     CDS             1762..2460
                     /gene="rplA"
                     /locus_tag="PLY05_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HNC03158.1"
                     /translation="MAKLTKRQKAIKAVVQAGKAYGIEEAVNVLSSLPPAKFKESIDI
                     AVNLGVDPRKSDQVVRGASVLPHGTGKTVRVAVFAQGANAEAAKAAGADIVGFDDLVA
                     SVQAGNLDFDVVIATPDAMRVVGKLGTILGPRGLMPNPKVGTVTTDVTTAVKNAKGGQ
                     VRYRVDKGGIIHTTVGQVGFSAQAIKENVESLIADLKKAKPATSKGAYVKKVTLSTTM
                     GPGLAIDQSSLATA"
     gene            2804..3304
                     /gene="rplJ"
                     /locus_tag="PLY05_07370"
     CDS             2804..3304
                     /gene="rplJ"
                     /locus_tag="PLY05_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNC03159.1"
                     /translation="MTLKLEDKKEIVAEVNKVASGAFSAVVADYRGLTVGQLTKLRTK
                     AREQGVYVRVIRNTLARRAVEGTNFACLQDSLVGPSIIGLSLSENDMGAAARLFKDFA
                     KDNQKLVVKALGFDGKLYDAKDIDRIASLPNHKQALSMLLSVIQAPVSKFGRLMTALK
                     EQKEAA"
     gene            3358..3729
                     /gene="rplL"
                     /locus_tag="PLY05_07375"
     CDS             3358..3729
                     /gene="rplL"
                     /locus_tag="PLY05_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNC03160.1"
                     /translation="MALSKDEILNAVAELTVMELVDLIAAFEEKFGVSAAAVAVAAGP
                     AAAAAPVEEQTEFNVVMTSFGANKVNVIKAIREITGLGLKEAKDMVEGAPSVVKEGIS
                     KDDAAALKKKLEEAGATVEVK"
BASE COUNT         1158 a          782 c          946 g         1223 t
ORIGIN      
        1 gtgaaggcgg ccgtacagtc ggtgctggtg ttgttgctaa aatcatcaag taattgatgt
       61 gttagacttg tgatggtaaa ctatcacaag tcagcaagat taaggcttgg tgcagctaag
      121 cctttaatcg gattaaaatt taggccagta gttcaattgg tagagcaccg gtctccaaaa
      181 ccgggtgttg ggggttcgag tccctcctgg cctgccaaat tcctatctaa taagctgacg
      241 gtttatgacc acacaagccg aagacgtaac tacgaataat agtcttaaca ctgtcaaatg
      301 ggtggtggct attgctattc ttattgcggc aaccgtgggt aatcgttatg cgcccgagtt
      361 gcttccgcaa ttatcttcat gggttcgcat tgttgcactt gttgtattgg ctgtaggtgc
      421 tttagcaatt acctttacaa caacacaagg ccaatccttt attaagctct taaaagaagc
      481 ccaagttgaa gcccgtcgta ttgtttggcc aactaaagac gaaacgatgc agacaacgat
      541 gattgtttgt gctgttgtcg tagtgatgtc tttattgttg tggggcattg ataccctatt
      601 tggttggatg atttcggctg ttattggcta atagaagagg catttatgag taagcgttgg
      661 tatatcgttc atgcctattc aggcttcgaa aagcaagtca tgcgttcttt gcaagagcgt
      721 attaagcgtg ctgaaatgga acatctgttt ggtgaagtat tagtaccaac agaagaagtt
      781 gtcgaaatta aaaatggtgt taagcgcaag agtgagcgta aattctttcc aggttatgtg
      841 ttggtgcata tggaaatgaa tgatgactct tggcacttgg ttaaagattg tccaaaggta
      901 atgggtttta ttggcggtaa agcagataag ccagcaccaa ttactgacaa agaagccgat
      961 gcaattttaa gtcgcttacg tcaaagtaca gatgcaccac gtccaaaaac cttgtttgaa
     1021 ccaggtgaag tggtacgtgt tgttgatggg ccttttgctg actttaatgg ggcagtggaa
     1081 gaagttcatt acgagaagag tcgtttacgt gttgcggtca tgatctttgg tcgtgcaaca
     1141 cctgttgatc ttgaatttaa tcaggtcgaa aaagtttctt aagaacactt gttttatgtg
     1201 ctgaacataa aacgaaacac gaacagtcct acgttaaatt atctgaaaag gtattttaag
     1261 gtttgggctg ttttttatca ctcttatatt cggggagcct tacggcgtta ttacccaagg
     1321 agtctattat ggctaagaag attgatgctt acgttaagct acaaatccca gcaggtaaag
     1381 ctaatccatc tccacccatt ggtccagcat tgggtcaacg tggtgtgaac attatggctt
     1441 tctgtaaaga gttcaacgca gcaacacaaa aaatggaagt gggtcaacca actccagttg
     1501 tgatcactgt ttactctgac aaaagcttta cctttaccat gaaaagccca cctgcaacct
     1561 tcttattaaa gaaagctgct ggtatcaaat ctggttctaa gcgtcctaac acccaaaaag
     1621 tgggtaaagt gactcgtgca cagttagaag aaatcgtcaa tatcaagaaa gctgacttaa
     1681 cagcagcaga tatggatgca gcggtacgta ccttgtctgg ttctgcccgt tctattggta
     1741 ttgaggtaga gggttaataa catggctaaa ttaactaagc gtcaaaaggc gattaaagcc
     1801 gttgttcaag caggcaaagc ttacggtatc gaagaagcgg ttaacgtatt aagctctttg
     1861 cctccagcaa agtttaaaga gtctatcgat attgctgtta acttaggtgt tgatccacgt
     1921 aagtctgacc aagtagttcg tggtgcttct gtattaccac acggtacagg taaaactgta
     1981 cgtgttgctg tgtttgctca aggtgcaaat gctgaagcag ctaaagcagc tggtgctgat
     2041 atcgttggtt ttgatgattt agtcgctagc gttcaagctg gtaacttaga ttttgacgtt
     2101 gtaattgcta cgccagacgc aatgcgtgtg gttggtaagt taggtacaat tttaggtcca
     2161 cgtggtttaa tgccaaaccc taaagttggt actgtaacaa ctgacgttac aacagctgtt
     2221 aaaaatgcta aaggcggtca agtacgttac cgtgttgaca aaggcggtat tattcatact
     2281 accgttggtc aagttggctt ctctgctcaa gcaatcaaag aaaacgttga atctttaatt
     2341 gctgatttga agaaagctaa accagcaacc tctaaaggtg cttatgtgaa gaaagtaact
     2401 ctttctacca cgatgggccc aggtttagcc attgaccaat cttctttagc aacagcttaa
     2461 gaaattaaac ttaggtgcgc tgagtgccta agttaaaaaa ttgcggattt ccgcagcaag
     2521 gactttgata cgcttcttag tttggactta cgcggttatc gcttatttgt tcacatgaca
     2581 accgttttaa acggtttaat gttcgcaaaa cgcgctaatt tcgatgaaat gcgtaagtcc
     2641 tgagcgtcaa agacctcagg cgtaagtgct tgtcacttgc ttaaaagcgg ttaataaacc
     2701 aaagcctgcg tagacgcggt gtggttcagg tttctgtacc cccgcgaatg gcttaaagcc
     2761 aaggtatttt acctttgtgc ttttttattc ttaggaggtt acagtgactc ttaaattaga
     2821 ggataaaaaa gagatagttg ccgaagtcaa caaagttgct tctggcgcat tctctgctgt
     2881 ggtcgccgat taccgtggtt taactgtagg tcagttaacc aagttacgta ccaaagctcg
     2941 tgagcaaggc gtatatgtgc gtgttattcg taacacctta gcacgtcgtg cagtagaagg
     3001 tactaacttt gcttgcttac aagactcgtt agttggtcca agcattattg gtctctctct
     3061 gtctgaaaat gacatgggtg cagccgctcg tttgtttaaa gattttgcta aagacaacca
     3121 aaaattagtt gttaaagcat taggttttga cggcaaattg tacgatgcca aagacatcga
     3181 cagaattgct tctttaccaa accacaaaca agctctgtct atgttgttgt ctgtcatcca
     3241 agctccagtg tccaagtttg gccgcttgat gactgctttg aaagaacaga aagaagccgc
     3301 ttaatcgcag cttaacaaat ttaaccatac ccccacattt tattggagtg tattaacatg
     3361 gccttgtcca aagacgaaat tttaaatgcc gttgctgaat taacagtaat ggaattagtt
     3421 gacttaatcg ctgctttcga agagaagttt ggcgtttctg ctgctgctgt tgctgttgct
     3481 gctggtccag ccgctgctgc tgctccagtt gaagagcaaa cagaatttaa cgttgttatg
     3541 accagcttcg gcgcaaacaa agttaacgtt attaaagcaa tccgtgaaat cactggctta
     3601 ggcttaaaag aagctaaaga catggttgaa ggtgctcctt ctgttgttaa agaaggcatc
     3661 agcaaagacg acgcagctgc tctcaagaag aaattggaag aagctggcgc aacagttgaa
     3721 gttaagtaat cagcctaaat ttaggtgttt acttgcagct tcaaatgagc gcaacaatgg
     3781 ctggtggctg tttaagccgc cggccttttt ctgttttagt gcatggtaat ttagaaacga
     3841 tttgacaaga aacaattaag gctttatctc cacttgttaa tagatttggc ttagtttttt
     3901 cttgcaaata gtttaccaag caggctctag caaagtatta attttgcaag tgtttgtgat
     3961 agcagtctcg ccagtgtttt actggttttc attcgagttt agtctagctg aggactccag
     4021 atggcatact catacaccga aaaaaaacgc atccgaaaaa acttcggcaa attgcccacc
     4081 ttaatggatg tgccacattt attaggcat
//
LOCUS       DAOQMB010000199         4106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10781_length_4106_cov_11.421871, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000199 DAOQMB010000000
VERSION     DAOQMB010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10781_length_4106_cov_11.421871"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..3220)
                     /locus_tag="PLY05_07380"
     CDS             complement(158..3220)
                     /locus_tag="PLY05_07380"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HNC03161.1"
                     /translation="MARMKIFNTLEQAAFDSPPVFNSVERKQFFALPLILKDVIIKLR
                     TPTNKVCFLATVGYFKARRKFFAGQVNVADIEYIARQLNLSPTEVDVSTYTKFACHHH
                     QRIILDYFGCRPFDVKTMAFAKSEIAILVPVQYQPKKILIEIIQILTREKIEVPSYNI
                     LADLIISAINQHKRKLSQIIDQSLSEKQRGLLDALLEKEIDNSTMEGWHYRLTLLKRP
                     SQSTLPAKIKANLADLDALQTLYLDIRPIISQLHLGHECIKYYAYSVIKSQIPQVSRR
                     AAADRRLHLIAFVAYQTFKLNDTLIDTLLNVVQATANSAEKAQKDAYFQAREQRSQSL
                     VLLTDKLRHSIRETLSSIKDIVANADLSDSQKVAMIDIALSAQPAKATLETKLDDFKR
                     QTTILYQGQDYLSSLELNSLKLQLRVADIIRKIQFSPNCSLPNLWMALVYYQQREGHI
                     DKQAPVDFLSAEERMALTTTEGRFRVSLYKALLYVRIADAIKSGALNLVHSEKYRAFD
                     EYLIPKVDWQNARANYLHRAQLEEFVDCKTTLHQLGEAVDKGYSKLNQRLSSEQKTYL
                     TLRKDGSFHVSTPKKEDVDCAPLGSFFPERKYISMLEMLATVDKASNFLDEFEHWQVK
                     YQRAKPSKKLFMAGVIGFGCDIGHRKLAQTSKQIDEGDLDNVINWYFSLQNVQNANDR
                     ILRLLDKMNLPNIYRSDADILHTSSDGQKFEVAVDSLNANYSFKYLGKDKGVSVVTFI
                     DMRDLMWHSAVISSSEREAAYVIDGLMHNDVIQSDIHSTDTHGYSEVIFAATHLLHVG
                     FAPRIKNLGHQHLYAFKKRQHYQDLNYTLLPDKTIREDLIVQQWDEILRFVATIRLKM
                     TTASQLFKRLNSYSRQHPLYSALKEFGKIPKTLFILQYCDDLPLRQAIEKQLNKGEGS
                     NKFSRAISFGHNQEFTYGEKEDQEIAEGCRRLIKNAIVCWNYLYLARALADEKDEERK
                     SALIEAIRNGSVTTWKHFNLHGEFDFSDERMIDSMGLIPPRNISIKRE"
     gene            3380..3994
                     /locus_tag="PLY05_07385"
     CDS             3380..3994
                     /locus_tag="PLY05_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC03162.1"
                     /translation="MSKTIAYLRVSTADQDLEKNKGDILFFANHHNLGQVTFVEEVVS
                     GRKPWRERHIANILEQLQAGDVMIVAELSRLGRSMLECMEILALATQKRIRVYSVKGD
                     WQLDDSIQSKIIALVFSMAAEIERDLISKRTTEALRFKKAQGMTLGRPKGIGKSKLDV
                     FRPEIEGLLANGATQKFIARRYHTTEANLHHWLKRHGLKKAKPI"
BASE COUNT         1224 a          791 c          825 g         1266 t
ORIGIN      
        1 gtagttcttt ggtgaggttg tcggcatatt tgcttttgta ttcttcgctg tgcagcaaac
       61 cgtagggtgc atttaaacaa acgacagaaa agtagcatag gatttcacgg aaactaaatg
      121 ataccaatgc cttcaacagg ttttcgacct cccattttca ctctcgttta atactgatat
      181 ttctgggagg tatcagcccc atagagtcga tcatccgttc atccgaaaaa tcaaactccc
      241 catgcaagtt aaaatgcttc caagtcgtga ctgagccatt gcgaattgct tcgattaacg
      301 cagatttgcg ctcttcatct ttttcatctg ccaatgcccg tgcaagatat aaataattcc
      361 aacagacgat ggcattttta attaaacggc gacagccttc agcaatttct tggtcttctt
      421 tttcgccata ggtaaattcc tgattgtgcc caaaagaaat agctcgcgaa aatttatttg
      481 atccctcacc cttgttaagt tgtttttcaa tagcctgcct taatggtaag tcatcgcagt
      541 attgtaaaat gaacaatgtt ttgggtattt tgccaaactc ttttaacgca ctatatagcg
      601 gatgttgtcg agaataagaa tttaaccgct taaatagctg agaagctgtg gtcattttta
      661 accgaatagt ggccacaaag cgcagtattt catcccattg ctgcacaatt aaatcctcac
      721 gaattgtctt atctggcaat aaagtatagt ttaagtcttg ataatgctga cgttttttaa
      781 atgcgtacaa atgctgatgc cctaagtttt taatgcgtgg tgcaaaacca acatgcagga
      841 gatgagttgc agcaaagata acctctgagt aaccatgcgt atccgttgaa tgaatatcac
      901 tttgaatcac atcattgtgc atcaaaccgt caatcacgta tgctgcttcg cgctcagagg
      961 aactaatgac agcggaatgc cacattaaat cacgcatatc aataaatgtg acaacactca
     1021 ctcccttgtc tttacctaaa tatttgaatg aataattcgc attcagcgaa tcaaccgcaa
     1081 cttcaaattt ttgcccatca ctagaagtat gtaatatgtc agcatcgctc ctgtaaatat
     1141 tgggcaagtt cattttatct agcaatcgca atatgcggtc attggcgttt tgtacgtttt
     1201 gcagtgaaaa ataccaatta atgacgttat ctaagtctcc ctcatcaatt tgtttagagg
     1261 tttgtgccag tttacgatgc ccaatatcac aaccaaagcc aatcacacct gccataaata
     1321 gctttttgct tggtttagcg cgttggtatt tgacttgcca atgctcaaat tcatccaaaa
     1381 aattgcttgc cttatccacc gtcgctagca tctctaacat tgagatatat tttctctctg
     1441 ggaaaaaaga gcctaaaggt gcacaatcaa catcctcttt tttaggtgtg ctaacatgaa
     1501 acgacccatc ttttcgcaat gtcaggtacg ttttttgttc agagctaaga cgttgattta
     1561 atttgctata ccccttgtca accgcctcgc ctagttgatg aagtgttgtc ttgcagtcaa
     1621 caaattcttc tagttgcgca cgatgtaaat agtttgcacg ggcattctgc caatccactt
     1681 tcggaattaa atattcatcg aaagcacgat atttttcaga atggactaaa ttcaatgcgc
     1741 ctgatttaat tgcatcagca atacgaacat acaataaagc cttgtagaga gatacgcgaa
     1801 atctaccttc cgttgtggtg agtgccatcc gttcctcagc agacagaaaa tcaacggggg
     1861 cttgtttatc aatatgtcct tctcgttgct gataatacac cagtgccatc cataaattgg
     1921 gaaggctaca gttaggcgag aattgtattt tgcgtataat atcagccaca cggagttgaa
     1981 gtttaagcga gttcagttct agtgaggaca aataatcttg gccttgatac agtatggtcg
     2041 tttggcgttt aaagtcatcc aatttagtct ccaacgtggc cttagcaggc tgcgcactca
     2101 gggcaatgtc aatcatagcg actttttggc tatcactcag atctgcatta gccacaatat
     2161 ctttaatgga tgacaacgtt tcacgaattg aatggcgtaa tttatcggtt aatagaacca
     2221 gagattggct gcgttgttct cgtgcttgaa aataggcatc tttttgagct ttttcggcac
     2281 tattggctgt tgcttgtaca acgtttaaca acgtatcaat cagtgtgtca ttcagtttaa
     2341 aggtctgata cgctacaaat gcaatgaggt gtaagcgtcg gtcagcagca gctcgacgtg
     2401 atacttgggg aatctgtgat ttaatcaccg aataggcata atatttaata cattcatgac
     2461 ccaagtgaag ttgtgaaatg ataggtctta tgtctaaata gagcgtttgc agagcatcta
     2521 agtcggctaa attagctttg attttcgcgg gtaatgtcga ttgtgagggg cgttttaaga
     2581 gagtaaggcg ataatgccat ccttccatcg ttgaattatc gatttctttt tctaacagtg
     2641 catcaagcag ccctcgttgt ttttcactga ggctctgatc gataatttga ctgagtttac
     2701 gcttatgttg attaatggct gaaataataa gatctgccaa tatattgtaa ctgggtactt
     2761 cgattttttc acgagtaagt atctgaataa tttcaataag tattttttta ggttgatatt
     2821 gaacaggtac caaaatagct atttcgcttt tagcaaaagc cattgttttt acatcaaaag
     2881 ggcggcatcc aaaataatct aggataatgc gttgatgatg atgacacgca aatttagtat
     2941 aggtagagac atcaacctct gttgggctaa gattcagttg acgtgcaata tactcaatat
     3001 ctgcgacatt aacctgacct gcaaagaatt tccgtcgagc tttaaaataa ccaacagtgg
     3061 ctaaaaaaca aaccttgtta gtgggtgtgc gtagtttgat gatgacatct ttcaggatga
     3121 ggggtaatgc aaaaaactgc ttacgctcga cactgttaaa gactggggga gaatcaaagg
     3181 cagcttgctc cagtgtattg aatattttca ttcttgccat tagatgaact caccatagaa
     3241 tcgaaaacga taaaaatatt attgttaaaa tgaacattta tacaataatt tatagttagc
     3301 acaactcatt gatattgaaa taacgaaaac tataaaataa atcagatatt ttggttttta
     3361 ttaaatagca ggcaatggca tgagtaaaac aattgcttat ctgcgtgttt caaccgcaga
     3421 ccaagacctt gagaaaaata agggcgatat tttatttttt gctaaccatc ataatttagg
     3481 tcaagtaacg ttcgttgaag aagtagtttc gggtcgtaag ccttggcgag agcggcatat
     3541 tgccaacatc cttgagcaat tacaggcagg tgatgtgatg attgtcgcgg aactgtcacg
     3601 cttaggccga agtatgttgg agtgtatgga aatactggcg ttggctacgc aaaaacgtat
     3661 tcgagtgtat tccgttaaag gggattggca gctagacgat agcattcaga gtaagattat
     3721 tgccctagta ttttcgatgg cagctgaaat tgagcgcgat ttaatttcta agcgaacaac
     3781 agaagcatta aggttcaaaa aggctcaagg aatgactttg ggtcgtccta aaggtattgg
     3841 caaaagtaaa ttggatgttt ttagaccaga aattgaaggc ttattagcaa atggagcaac
     3901 tcaaaagttt attgcaaggc gttatcacac aacggaagcc aatttacacc attggcttaa
     3961 aaggcatggg ttaaaaaagg cgaagcccat ttaaccgtta ttcagctata aaatcctatg
     4021 ctacttttct gtcgtttgtt taaattcacc caagctatgg cacttggtaa acattccacc
     4081 acaagaccat gatgccgtta tcgctt
//
LOCUS       DAOQMB010000200         4104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10790_length_4104_cov_15.942949, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000200 DAOQMB010000000
VERSION     DAOQMB010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10790_length_4104_cov_15.942949"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1346)
                     /locus_tag="PLY05_07390"
     CDS             complement(120..1346)
                     /locus_tag="PLY05_07390"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HNC03163.1"
                     /translation="MEKLSSIVSRVANQTYGHSRRFLPEQPWWNSLEPSFHKFPDDFY
                     LDFKTQMMVEGTAALDIGLRTAMASLASVCCLPFTLSPKQLAEDYQDRFFYQKLGDTH
                     DPTQFFKKPTEKVVVHRHPAGVMDYKPDDGGRCELLSFESPFETVNPKKREDYAKLTH
                     NRMAWAQHWQHGDKPRPTICMIHGFMADPYWFNSKWLALPWFYKQGYDILLYTLPFHG
                     RRRAPLEPFGGYGYFAHGILHINEAVAHAVYDFRLFMDYLESTGVEKMGVTGISLGGY
                     TSAVLAAVEDRLQFCMPNVPVVSLVDLLYEWFPVAPLVKTGLRMAGINIHEARHTLSV
                     QCPLTYKPKLPKEQLMIISGAGDRLAPPKHSRLLWEHWDRCRLHWFPGNHILHLDQGK
                     YLKDMAQFLRDIEFNK"
     gene            1501..1836
                     /locus_tag="PLY05_07395"
     CDS             1501..1836
                     /locus_tag="PLY05_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03164.1"
                     /translation="MSQDNQQEGAFLLSLKRNNDKIREDRALAIAEDAQLIFKREIED
                     MAIQLKRLQRERDGLLDLSPTTADSLVLANDFDAKTFVEKDLQLGIQMRNLEIRLEIA
                     KQRYAFLFV"
     gene            1854..2699
                     /locus_tag="PLY05_07400"
     CDS             1854..2699
                     /locus_tag="PLY05_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03165.1"
                     /translation="MGYTHYSVNDRRQRAEAAGFATRSRDEIFSQQKSQSIHESMDPR
                     KALLRESRDSATHPNSIPIIIALDVTGSMGQIPHSLVIDGLPTLMSTIIQHGFPDPQI
                     LFMAIGDQECDRFPLQVGQFESGDAELDLWLTRTYLEGGGGGNGGESYHLAYAFAGLR
                     TAHDAWDKRKQKGLLFTIGDEPCLSHLPPVVLREVMLEESQQTWTVEQLLQLVQQKYD
                     VFHLHLLQGSAGSRSLGYWQQLLGQHCLVIDNISKIPQVIAQTVIQHAQQYATKKPTV
                     SELML"
     gene            2696..3787
                     /locus_tag="PLY05_07405"
     CDS             2696..3787
                     /locus_tag="PLY05_07405"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNC03166.1"
                     /translation="MKQQAKIVLGLAFGDEGKGLSTDYLCQQYPNSLVVRFSGGQQCG
                     HQVRIGQERHIHSNFGSGTLRGLPSFFTEHCCFYPVTLLNEYKILINKGVSPVLYLHP
                     LCKLTTPFDVLANRFSERQKTHGSCGLGIGKTMQREQMGYHLFAIDTLYPELLSQKLN
                     LLRHYYFQHYSFSSDELTWLSQELALFQQAIIDRPYQIADYDLWQQYSHFIFEGSQGI
                     LLDQKHGLFPHVTYANTTSKNAIELCQRFNIAYEIFYVTRCYGTRHGKGWLAKETDQL
                     NIVANPHETNQFNDYQGEFRLAEIDYSSLNYALAVDAIYAQHCPKHLVVTCLDQRPDF
                     TFDYERLNTTFNHIYESYSDNSDNLQVIK"
     gene            3866..3942
                     /locus_tag="PLY05_07410"
     tRNA            3866..3942
                     /locus_tag="PLY05_07410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3900..3902,aa:Pro,seq:ggg)
BASE COUNT         1287 a          860 c          738 g         1219 t
ORIGIN      
        1 gaccccagca ttatgagtgc tgtgctctaa ccaactgagc tacatagcca tctactcaac
       61 aagtgaggcg catattttct ttataaacca acatcatgtc aagcatttat taactaaaat
      121 tacttattaa actcaatatc gcgcaaaaat tgagccatat cttttaaata cttcccttga
      181 tctaaatgca aaatatgatt cccaggaaac caatgcaaac gacatctatc ccaatgctcc
      241 cacaacaaac gactatgctt gggtggtgca agtctatctc ctgccccact aataatcatt
      301 aattgctctt tgggtagttt tggcttatag gtgagcgggc attgtacgga caaggtatga
      361 cgagcctcat gaatattaat acccgccata cgcaagccag ttttaactaa aggggcaaca
      421 ggaaaccact catacaacaa atcaaccaaa ctcactacag gcacattagg catacaaaac
      481 tgtaatctat cctccactgc tgctaatacc gcactggtat atcctcctaa agaaatacct
      541 gtcacaccca ttttttccac acctgtactt tctaaataat ccataaataa acgaaaatca
      601 taaaccgcgt gagccactgc ctcattaata tgcaaaatac catgagcaaa atatccataa
      661 ccgccaaaag gctctagagg tgcacgacga cgaccatgaa atggcaaggt atataataaa
      721 atgtcataac cttgcttata aaaccaaggc aaagctaacc atttactatt aaaccagtaa
      781 ggatcagcca taaaaccatg aatcatacaa atggttggtc ttggtttgtc accatgttgc
      841 caatgttgcg cccatgccat acggttgtga gttaatttgg cataatcctc acgctttttg
      901 ggattcaccg tttcaaaagg actttcaaac gataacaact cacagcgacc accatcatca
      961 ggtttataat ccatcacgcc tgctgggtgt cgatgtacca ccactttttc ggttggcttt
     1021 ttaaaaaatt gtgttggatc gtgagtatca cctaatttct gataaaaaaa tcgatcttga
     1081 taatcctctg ctaattgttt gggtgataac gtaaatggta aacaacaaac actggctaac
     1141 gaagccatgg ctgtccgtaa accaatatcc aaagctgctg tcccctcaac catcatttga
     1201 gttttaaaat ctaaataaaa atcatctgga aacttatgaa agctaggctc taaagaattc
     1261 caccacggtt gctcaggcaa aaagcgacga gaatggccat aggtttgatt ggcaacacgc
     1321 gaaacaatag aactcagctt ttccatagtc ttaaactcaa cttattggac gatagtacgg
     1381 taaaatagcc tatcttctac tctgctgaca agccttaaaa atattttcat ttatttattg
     1441 tctttaggac actaattatc tatactcact tcaatacaaa cataaatagg agtcttcatc
     1501 atgagtcagg acaatcaaca agaaggtgct tttttactat cactcaaacg taataacgac
     1561 aaaattcgag aagatcgtgc actggcaatt gcagaagatg cccaacttat ttttaagcgt
     1621 gaaatagaag acatggccat tcaactgaag cgtctgcagc gtgaacgtga tggtttatta
     1681 gatttatcgc ctaccacggc ggatagtttg gttttagcca atgattttga tgccaaaacc
     1741 tttgttgaaa aagatctaca gttaggcatt caaatgcgta atttagaaat ccgcctcgag
     1801 attgctaaac aacgttatgc ctttttattt gtttaattaa atggagaacc atcatgggat
     1861 acacacacta ttctgttaat gatcgtcgtc aacgtgctga agctgctggc tttgccacac
     1921 gtagtcgtga tgagattttt agccaacaaa aatcacaatc catccacgaa tctatggatc
     1981 cacgcaaagc tttattaagg gaaagtcgcg attcagccac tcaccccaac tctattccta
     2041 ttatcattgc cttagatgtg acgggttcaa tgggacaaat tcctcatagt ttggtgattg
     2101 acggcttacc aaccttaatg tcgaccatta ttcaacatgg ttttcctgat ccacaaattt
     2161 tatttatggc aattggcgat caagaatgtg accgtttccc actacaggtt ggccaatttg
     2221 aatctggtga tgctgagtta gatttatggc taacacgcac ctatttagaa ggcggtggtg
     2281 gtggaaatgg tggtgaaagt tatcacctag cctatgcttt tgcaggttta cgcactgcac
     2341 atgatgcttg ggataaaaga aaacaaaaag gtttgttatt taccattggt gatgaacctt
     2401 gtttgagtca tttaccccct gtagttttac gcgaagtcat gcttgaagaa agccaacaaa
     2461 cgtggacggt tgaacagcta ttacagttag tacaacaaaa atatgatgta tttcatttac
     2521 atctattaca aggctctgcg ggaagtcgtt cattaggcta ttggcaacag ctattaggtc
     2581 agcattgttt agttattgat aatattagca aaataccaca agtgattgca caaacagtga
     2641 ttcaacatgc tcaacaatat gccactaaaa aaccaaccgt atctgagctg atgttatgaa
     2701 acagcaagca aaaattgttt taggcttagc cttcggtgac gaaggcaagg gtctaagcac
     2761 agactattta tgtcaacaat atcccaatag ccttgttgtc cgcttttctg gcggacaaca
     2821 atgtggccat caagtgcgta ttggtcaaga acgacatatt cactcaaatt ttggcagtgg
     2881 tacattgcgt ggtctgccct cttttttcac agagcattgt tgcttttatc ctgtgacttt
     2941 actcaatgaa tataaaatat taatcaataa aggcgtgtca cctgttttat atttgcatcc
     3001 tttatgcaaa ttaaccacgc cttttgacgt attagccaat cgctttagtg aacggcaaaa
     3061 aactcatgga agttgtggtt taggcattgg taaaaccatg cagcgcgaac aaatgggtta
     3121 tcatttattt gccattgata ctctctatcc agagttatta agccaaaagc ttaatttact
     3181 gcgtcattat tattttcagc actatagctt tagctctgac gaattgacgt ggttaagtca
     3241 agagctcgcc ctttttcaac aagccattat tgaccgtccc tatcaaatcg ctgattatga
     3301 cttatggcaa caatattcac attttatttt tgagggttca caaggcattc ttcttgatca
     3361 aaaacatggc ttgtttcccc atgtaaccta tgccaacacc accagcaaaa atgctattga
     3421 gctatgtcaa cggtttaata ttgcttatga aattttttat gtgacgcgtt gttatggcac
     3481 acgtcatggt aaaggttggt tagccaaaga aaccgatcaa cttaacatcg tggctaaccc
     3541 acatgaaacc aaccaattta atgactatca aggtgaattt agattagcag aaatcgatta
     3601 ttcatcttta aattatgctt tagcggtcga tgccatttat gcccaacact gcccaaaaca
     3661 tttggtagtg acttgtttag atcagcgacc tgattttaca tttgattatg agcgattaaa
     3721 tactactttt aatcatattt acgagtctta ttcagataac agtgataatt tacaagttat
     3781 taagtaagtg gtttattcct taaacagtta agcatgacaa aatactagac aaatcatcaa
     3841 aatccgctaa tatagcagcc ctcttcgggg cgtagcgcag cctggtagcg cacttgcatg
     3901 gggtgcaagg ggtcgcgagt tcaaatcccg ccgtcccgac caataaaatc aaagggttat
     3961 gtttaacgac ataacccttt tttattgaaa aaggcattta gggtatctat tagggtatct
     4021 ttcaaaattc tataccccct aaaaatagca aaatcctact tggtggcagt agcttttaaa
     4081 gtcttcttaa tatcgtttag tttt
//
LOCUS       DAOQMB010000201         4072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_10952_length_4072_cov_16.258402, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000201 DAOQMB010000000
VERSION     DAOQMB010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10952_length_4072_cov_16.258402"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PLY05_07415"
     CDS             complement(<1..261)
                     /locus_tag="PLY05_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC03167.1"
                     /translation="MGAQWKAKHKEVAANAKGKIFGKLSKEIMVAARNGADPAMNPRL
                     RLAVEQAKKASMPRDTLERAIKKGAGLLDGGVNFEKVTYEGFA"
     gene            complement(292..1089)
                     /locus_tag="PLY05_07420"
     CDS             complement(292..1089)
                     /locus_tag="PLY05_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HNC03168.1"
                     /translation="MWELLWPAWLAGSLLALVAAPLGCLLVWRRLAYFGDSLSHATLL
                     GVALSLWLHIPVWGGLVAVCVMIALILASLLAKRQLPSDALLMAVSTTTLSAGLIAIS
                     KMDEVRVDLLSYLFGDLLSISSTDLPVFVSCLGLVLLGLAYFWRDLLLTTIDEELAFV
                     EGLPVTKLRLLLLILLALVVTVAMKAVGSLLITALLVIPSLVARPLVNTPTQMVLLAA
                     MTGVTAVSGGLLSSFYWDTPVGASIVVSAALLFVLAQVKVLMTRLKA"
     gene            complement(1093..1860)
                     /gene="znuC"
                     /locus_tag="PLY05_07425"
     CDS             complement(1093..1860)
                     /gene="znuC"
                     /locus_tag="PLY05_07425"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HNC03169.1"
                     /translation="MTEILVELKQVQLVAEQRTILRDIDLVLPRGQIVTLIGPNGSGK
                     SSLVKIVVGLQKASAGQRQLSAKLRIGYMPQALKLDKSLPLTVRHFLQLSRTKPSPIE
                     LLIALNDVGAEPLLEQSIHTLSGGEWQRVLLARALLQKPDLLVLDEPMQGVDLTGQEE
                     LYNLIAQLPEKYHCGVLMVSHDLHLVMAATDSVICLNGHICCHGHPESVSNHPEYLRL
                     FGLQGSKGLAVYSHHHDHQHDAHGCVHPHHSPVKQES"
     gene            complement(1853..2332)
                     /locus_tag="PLY05_07430"
     CDS             complement(1853..2332)
                     /locus_tag="PLY05_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNC03170.1"
                     /translation="MSQHAFHPHHHEHCVHDALQSAEVLCAERGVRLTAIRKRVLELV
                     WASHKPLGAYAILDKLTDEGHKPAPPTVYRALEFLLEQGLIHRIASLNAFLGCVHPSE
                     QHLACFFLCQQCGNAEELQNSDLLQQELQQRAASTGFLIRQPILEVTGLCKQCQHHD"
     gene            2414..>2748
                     /locus_tag="PLY05_07435"
     CDS             2414..>2748
                     /locus_tag="PLY05_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNC03171.1"
                     /translation="MSIKHFFATVSLLVISNAASAQIVTTIKPLTLIAQEVTQGIEQP
                     IQLLPVGASAHHYALKPSQRVLLQKANLIVWVGNSHETFLQTTLKPLSNVLTFEQLPN
                     LKKLAWRDL"
     assembly_gap    2749..2848
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2889..3980)
                     /locus_tag="PLY05_07440"
     CDS             complement(2889..3980)
                     /locus_tag="PLY05_07440"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC03172.1"
                     /translation="MNLLDLLLSTDKKQRLRIRRSLLASLVFLISIGMMIYLSYIGKM
                     SIQATSLLSLGMISTCLAFYLALRTDFNLRFKEPALTLPQILVAMTWICAAYANTGQA
                     HAGTLMLYALVMVFGIFVMEPRRSVISASYGVLAMVSTMLYKMLTDSQAYPWQIEIVY
                     LLLVVTILPTIARLSEQLSAMRRRLKIQKNDLEQACTQLERVAAYDELTGLINRRRMT
                     EVLAEHSRNQTRQTEGFAIAMIDLDYFKMINDKYGHAIGDQVLSVFGESARKVLREID
                     ILSRWGGEEFLLLMPKTNNGHPNIGIERLRAYLACVQPCPQIPELKISFSVGFTSYEK
                     GESIYDTIKRADGALYEAKAKGRNCTVII"
BASE COUNT         1294 a          930 c          672 g         1076 t
ORIGIN      
        1 ggcaaaacct tcgtaggtaa ctttttcaaa gttaacacca ccatccaaca agccagcccc
       61 ttttttaatc gcacgttcta gggtatcacg tggcatagag gcttttttag cttgttctac
      121 cgctaaacgt aaacgtggat tcatggcagg atcagcacca ttacgcgccg caaccataat
      181 ttctttagac aatttaccaa aaattttacc tttggcattt gccgcaactt ctttgtgctt
      241 ggctttccac tgtgcgccca taattgttct cttttaaaaa taatacctaa atcaagcttt
      301 caagcgagtc atcaacactt tgacttgagc taaaacaaat aacaacgctg cgctgacaac
      361 aatacttgcc ccaacaggcg tatcccaata aaaactcgat aacaagccac cactcacagc
      421 cgttacccct gtcattgccg ctaataatac catttgtgtg ggcgtattga ctaagggtcg
      481 cgccactaaa ctaggaatga ctaataacgc ggtaattaac aaactaccga cagccttcat
      541 cgccaccgtc accaccaaag ccagcaaaat gagcaataat aaacgtaatt tggtgacagg
      601 taggccttca acaaaagcca actcttcatc aattgtagtc aataataaat cacgccaaaa
      661 ataagctaac cccaacaaca ctaagcccaa gcaactgacg aacacaggta aatcggtact
      721 actaatactt aataaatcac caaacaagta acttaacaaa tctactcgca cttcatccat
      781 tttactaatg gcaatcagac ctgcactcaa agttgtcgtt gataccgcca ttaacaaagc
      841 atctgacggc aattgtcgtt tagccaacaa acttgccaaa atcaaagcaa tcatcacaca
      901 aaccgccacc aatccgcccc aaacaggaat atgtagccac aagcttaagg ccacacctaa
      961 taaggttgcg tgcgacaaac tatcgccaaa atatgccaaa cgtcgccaca ccaacaaaca
     1021 acctaagggt gcagctacta aggccaataa cgaacctgct aaccatgcag gccataataa
     1081 ttcccacatg atttatgact cttgtttaac aggtgaatgg tgcggatgaa cacaaccatg
     1141 agcatcatgt tgatggtcgt gatggtgact atacactgct aagcctttgc taccttgcaa
     1201 accaaacaaa cgcaaatact caggatgatt actgactgac tctggatgac catgacaaca
     1261 aatatggcca tttaaacaaa tcacactatc ggttgccgcc atcaccaaat gcaaatcatg
     1321 cgagaccatt aacaccccac aatgatattt ttcgggtaat tgggcaatca ggttataaag
     1381 ctcttcttga cctgttaaat ctaccccttg cattggctca tctagcacta acaaatcggg
     1441 tttttgtaat aaagcacgcg ccaataagac tcgttgccac tcaccccccg ataaagtatg
     1501 aatcgactgc tctaataacg gttctgcgcc tacatcattt agagcaatta ataattctat
     1561 agggctaggt ttagtgcgac ttaactgcaa aaaatgtctt actgttaatg gtaaagattt
     1621 atccaattta agggcttgcg gcatataacc aatacgcaat ttagcggata actgtcgctg
     1681 tcctgcacta gctttttgta aaccaacaac aatcttcact aaagaggatt tacccgaacc
     1741 atttgggcca atcagcgtca caatttgtcc tcgtggtaat accaaatcaa tatcacgcaa
     1801 aatcgtgcgt tgttcggcga ctaattggac ttgtttcagc tctactaaaa tttcagtcat
     1861 ggtgttgaca ctgcttacac agccccgtca cttctaaaat tggctggcga attaaaaagc
     1921 ctgtgctagc agcgcgttgt tgtaactctt gttgcaacag gtcgctattt tgcaactctt
     1981 cggcattacc acattgctga cacaaaaaga aacacgccaa atgctgctcg gagggatgca
     2041 cacagcctaa aaatgcattt aaagaagcaa tccgatgaat taagccctgc tctaataaaa
     2101 actctaaagc acgatatacg gtaggtggtg ctggcttatg tccttcatca gtcaacttat
     2161 cgagaatggc atacgcacct aatggtttat gacttgccca caccagttcc aacacacgtt
     2221 tacgaatcgc ggttaaacgc acaccgcgct cagcacataa aacctcggca gactgtaagg
     2281 catcatggac acaatgctcg tgatgatgag gatgaaaagc gtgttgactc ataaccgtac
     2341 tgatagtaaa aaaattaaat taatgcaata atataacatt ccatgctcaa ttcctaaagc
     2401 tgtattctct gttatgtcga tcaaacattt ttttgctact gttagtctat tagtcattag
     2461 caatgccgca tctgcacaaa ttgtcaccac catcaaacct ttaaccttaa ttgctcaaga
     2521 agtgactcaa ggcattgagc aaccaataca acttttacct gtaggcgcat ctgcacatca
     2581 ttacgctctt aaaccatcgc aacgggtgtt attacaaaaa gccaatttga ttgtttgggt
     2641 aggcaatagt catgaaactt ttttgcaaac aacgctaaaa cctttaagca atgttttaac
     2701 ttttgaacag ttaccaaatc tcaaaaaatt ggcatggcga gacttaaann nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnca aggttttggg caaatggctc aagcaattta
     2881 tgattgtatt aaataatcac cgtgcaatta cgccctttag ctttagcttc atacaatgct
     2941 ccatcagcac gtttaatcgt gtcataaata gactctcctt tttcgtaaga agtaaaacca
     3001 acagaaaagc taatttttaa ctcaggaatt tgtgggcaag gttgaacaca ggccaaataa
     3061 gcacgtaatc gctctatgcc aatattagga tgaccattat tggtctttgg cattaataat
     3121 aaaaactctt ctccacccca tcgactcaaa atatctattt ctcttaaaac ttttcgagca
     3181 gactcgccaa aaacagataa cacttgatcg ccaatagcat ggccatattt atcattaatc
     3241 attttaaaat aatcaagatc aatcatggca attgcaaaac cttctgtttg ccgagtttga
     3301 ttgcgactat gctcggccaa tacttcggtc atacgacgac gattaatgag tcccgtcaat
     3361 tcatcataag ctgcaacacg ctctaattgt gtacatgctt gttcaagatc atttttttgt
     3421 atttttaaac gacggcgcat ggccgataac tgctctgata agcgtgcaat cgtaggtaaa
     3481 attgttacca caagcagcaa atagactatt tctatttgcc aaggataggc ttgagaatcc
     3541 gttagcattt tatataacat ggttgatacc attgctaaca caccataact agcggatatc
     3601 accgaacgac gtggctccat gacaaaaatg ccaaatacca tgactaaggc atacagcatt
     3661 aatgtgcctg catgcgcctg acctgtattg gcataagcgg cacaaatcca agtcatcgct
     3721 actaaaattt gtggcaaagt taaggctggc tctttaaagc gcaaattaaa gtccgttctt
     3781 aaagcaagat aaaaagccaa acaagtgcta atcatgccta aagataataa gcttgttgct
     3841 tgtatcgaca ttttgccaat gtaagacaaa tatatcatca tgccaatact aatcaaaaat
     3901 actaagctgg ctaataaaga gcgtctaatg cgtaaacgct gttttttatc cgtagataac
     3961 aataaatcta ataaattcat tttgttgttt tctttttata gttcaggacc tacctatttc
     4021 accttcaggc gcacattttg tgagcataaa atcgttaaaa atacgagcca at
//
LOCUS       DAOQMB010000202         4018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11177_length_4018_cov_17.303810, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000202 DAOQMB010000000
VERSION     DAOQMB010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11177_length_4018_cov_17.303810"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1191
                     /locus_tag="PLY05_07445"
     CDS             16..1191
                     /locus_tag="PLY05_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004828905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC03173.1"
                     /translation="MSSVHVPEQVAPMIPRTLFNSDHEAFRKTARRFFEQEIAPFHEK
                     WEEQQHLDRDLWNKAGALGLLCPTMPEEYGGSGVDRLYSVIMMEEQARVGDSASGFSL
                     HSDIVANYINNFGNEAQKQEWLPKMASGEVVSAIAMTEPGTGSDLQSVQTRAIDMGDH
                     YVLNGSKIFITNGYLCDMAVVVAKTGNSEKGAQNISLLIVDAHSEGFSKGKPLKKVGM
                     KGQDTCELFFNNVKVPKENLLGMEGMGFIMLMKELAWERMMIGIMCAAAAEHALAHTV
                     EYVKGRKVFGKPVAAYQNTRFKLAELRSEIQIARVFVDRCIEQVLKHELSIEGACAAK
                     YWVSDLLSKVVDECVQLHGGYGFMLEYPIARAYIDNRANRIYGGTNEIMKELISRSI"
     gene            complement(1341..1679)
                     /locus_tag="PLY05_07450"
     CDS             complement(1341..1679)
                     /locus_tag="PLY05_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016541465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HNC03174.1"
                     /translation="MSSLPSCPQCNSEFTYEDGDLYICPECSHEWPKTAATASEDNRI
                     IKDANGNVLTDGDTVTVIKDLKVKGSSLVVKVGTKVKNIRLVDGDHDIDCKIDGIGAM
                     KLKSEFVKKV"
     gene            complement(1738..>2502)
                     /gene="metF"
                     /locus_tag="PLY05_07455"
     CDS             complement(1738..>2502)
                     /gene="metF"
                     /locus_tag="PLY05_07455"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNC03175.1"
                     /translation="VHQRLATLNPAYFSVTYGAGGSTRERTLSIVDQIQQAAPIAVAP
                     HLSCIGDNKSEIAELLHRYKNQGIKHLVALRGDLPSGQMGLGELPYARDLVEFVRQET
                     GDHFHIEVAAYPEMHPQSYDFDSDVKNFIAKVNAGANSAITQFFFNADAYFYFVEKVR
                     KAGVTIPIVPGIMPITNASNLIRFADGCGADIPRWIRKQLAAYGDDTASIKAFGEEVV
                     TKLCQTLLQGGAPSLHFYTMNQAEPTTRLCQLLDLA"
     assembly_gap    2503..2602
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2603..4003)
                     /gene="ahcY"
                     /locus_tag="PLY05_07460"
     CDS             complement(2603..4003)
                     /gene="ahcY"
                     /locus_tag="PLY05_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004648276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNC03176.1"
                     /translation="MTAMTSVGDYKVADISLAAWGRKEIILAEAEMPALMGLRRKYAA
                     SKPLAGAKIIGCIHMTIQTAVLIETLIDLGAEVRWSSCNIFSTQDQAAAAIAAVGIPV
                     FAWKGETEEEFWWCIEQTILKDGKPWDANMILDDGGDVTYIVHEKYPEMLKTIHGITE
                     ETTTGVHRLIEMWKKGTLKVPAINVNDSVTKSKNDNKYGCRHSLNDAIKRGTDMLMAG
                     RRALVIGYGDVGKGSAQSLRQEGMIVRVTEIDPICAMQACMDGYEVVSPYIDGNNTGL
                     DVDINKTLLGETDLLVTTTGNMNVCDAAMLRNLKNGAVVCNIGHFDTEIDTAYMRTNW
                     HWEEVKPQVHKVYRTNAADVNPADSNYLLLLSEGRLVNLGNATGHPSRIMDGSFANQV
                     LAQMYLYEQKFADHSPAVQERMLKVEVLPKKLDEEVAAAMVAGFGGVLTKLTPAQAEY
                     IGVSVDGPYKQESYKY"
BASE COUNT         1139 a          795 c          831 g         1153 t
ORIGIN      
        1 aataggagta atagaatgag cagcgttcac gttccagagc aagtcgcccc aatgattcct
       61 cgtactttgt ttaatagcga ccatgaggca tttcgtaaaa ccgcccgtcg tttctttgaa
      121 caagaaattg ccccatttca tgaaaagtgg gaagagcaac agcatttaga tcgtgactta
      181 tggaacaaag caggtgcttt aggtttgtta tgcccaacca tgcctgaaga atatggtgga
      241 tcgggtgttg accgtctata tagcgtaatt atgatggaag agcaggcacg tgttggtgat
      301 tctgcttctg gtttctcttt acactcagat atcgttgcca attatatcaa taactttggt
      361 aacgaagctc aaaagcaaga atggttgcca aaaatggcct ctggtgaagt ggtctctgcc
      421 attgcgatga ccgaacctgg tacaggctca gatttgcagt ctgtacaaac tcgcgccatt
      481 gatatgggcg atcactatgt tttaaatggc tctaaaatct ttattaccaa tggttatttg
      541 tgtgacatgg ccgtggttgt tgccaaaaca ggtaatagcg aaaagggcgc acaaaatatt
      601 tctttattga ttgttgatgc tcatagcgaa ggctttagca aaggcaaacc attgaaaaaa
      661 gtgggtatga aaggccaaga tacttgtgaa ttattcttta ataacgtcaa agtgcctaaa
      721 gaaaatttat taggtatgga aggcatgggc tttatcatgt taatgaaaga gttagcatgg
      781 gagcgcatga tgattggtat tatgtgtgcg gctgctgctg aacacgcgtt ggcacatacg
      841 gttgaatatg taaaaggccg taaagtgttt ggtaagccag ttgcagctta tcaaaatacc
      901 cgttttaaat tagcagaatt acgctctgag attcaaattg cgcgtgtgtt tgttgaccgt
      961 tgtattgagc aagtcttaaa gcatgagtta agtattgaag gtgcttgtgc tgctaagtat
     1021 tgggtgtctg atttgttaag caaagtggtt gatgagtgtg tgcaattgca tggtggttat
     1081 ggcttcatgc tcgagtatcc aattgcccgt gcctacattg ataaccgtgc taaccgtatt
     1141 tatggtggta caaacgaaat tatgaaagag ctaatttcgc gtagtattta aatcagtttc
     1201 ccctgttaat caggggagtt tgattaaagc agagttgtcg ttggtggttt tgacaacgac
     1261 ttcctcttct aaagtcttga gtattttaga agagggagct taagtctgta ggacgtatac
     1321 gactctcgtt agtcttgacc ttaaaccttt ttcacaaact cagatttgag cttcatcgcc
     1381 ccaatcccat caattttaca atcaatatcg tggtcaccat cgactaaacg aatattttta
     1441 actttagtac ccactttaac gaccaacgaa gaacctttaa ctttaaggtc tttaatcacg
     1501 gtgaccgtat cgccatcggt taaaacattg ccattagcat ctttaataat acgattgtct
     1561 tcgcttgctg ttgctgctgt ttttggccat tcgtgactac attcaggaca aatatataaa
     1621 tcaccatctt cataggtaaa ttcagaattg cattggggac aagatggcaa agagctcata
     1681 gaaaattcct agaaaaatgc cctctctttg gtggagaggg ttgaggtgag ggagggctta
     1741 agctaaatct aataactggc acaagcgagt agttggctcg gcttggttca tggtgtaaaa
     1801 gtgtaagcta ggtgcgccac cttgtaataa ggtttgacag agtttagtca caacttcttc
     1861 gccaaaggct ttaatactgg ctgtatcatc accataagcg gccaattgtt tacgaatcca
     1921 acgtggaata tctgcaccac aaccatcggc aaaacgaatt aaattgctgg cgttggtaat
     1981 gggcataata ccgggaacga tcggaatggt aacgcctgct ttacgtacct tttcaacaaa
     2041 gtaaaaatac gcatcggcat taaagaaaaa ttgggtaatc gcactattag cacccgcatt
     2101 aactttagca ataaaatttt taacatcact gtcaaaatca taagattgag gatgcatttc
     2161 gggataagca gccacttcaa tatgaaaatg atcgcctgtt tcttggcgca caaactccac
     2221 caaatcacgc gcatagggta attcgcctaa acccatttgg cctgacggta aatcaccgcg
     2281 taaggcgact aaatgtttaa tgccttgatt tttataacgg tgtaagagtt cagcaatttc
     2341 gcttttgttg tcaccaatac aagataagtg tggtgcaacg gcaatcgggg cagcttgttg
     2401 aatttggtca acaatcgaca atgtacgttc acgtgttgaa ccaccagcac cataagtcac
     2461 agaaaaatag gcagggttta aagtggctaa acgttgatga acnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnttagtact tataactttc ttgcttatat gggccgtcaa
     2641 cagagacgcc aatatattca gcttgagcag gtgttaattt ggttaacaca ccaccaaaac
     2701 ctgcaaccat tgctgcggca acttcttcat caagtttttt cggtaaaact tcaactttga
     2761 gcatacgctc ttgcacagca ggagaatggt cagcaaattt ttgctcatac aaatacattt
     2821 gggctaacac ttggttggca aaagaaccat ccataatacg agatgggtga cctgttgcat
     2881 tccctaaatt taccaaacga ccttcagata ataataacaa gtagttacta tcggctgggt
     2941 taacgtccgc tgcattagtg cgataaacct tgtgtacttg tggtttaact tcttcccaat
     3001 gccagttagt acgcatataa gccgtatcaa tttcggtatc aaaatggcca atattacaaa
     3061 ccactgcacc gtttttgagg ttacgcaaca tcgccgcatc acagacattc atattgcctg
     3121 tggtggtaac taataaatca gtttcaccta ataaggtttt gttgatatca acatctaaac
     3181 ctgtgttgtt gccatcaata tatggagaaa ctacttcgta accatccata caggcttgca
     3241 ttgcacaaat tggatcaatt tcggtgacgc gcacaatcat gccttcttga cgtaaagatt
     3301 gagcagaacc tttacccaca tcaccataac caatcactaa agcacgacga cctgccatta
     3361 acatatctgt gccgcgttta atggcatcat ttaaggagtg gcggcaacca tatttgttgt
     3421 cattttttga cttggtcaca gaatcattga cgttaatagc aggcactttt aacgtacctt
     3481 ttttccacat ttcaattaaa cgatgcacac ctgtggtggt ttcttcagta ataccatgaa
     3541 tggtttttaa catttctgga tatttttcgt gaacgatata ggtcacgtca ccaccatcat
     3601 ccaaaatcat gttggcatcc cacggcttac catcttttaa aatggtttgc tcgatacacc
     3661 accaaaattc ttcttcggtt tcacctttcc atgcaaagac gggaatgccc accgcagcaa
     3721 ttgccgcagc agcttgatct tgtgtggaga agatattgca agatgaccaa cgaacttctg
     3781 cccctaaatc aattaaggtt tcgattaata cggcagtttg aatggtcatg tggatacagc
     3841 cgatgatttt tgcacctgct aaaggtttgc tagcagcata cttgcggcgt aaacccatta
     3901 aggcaggcat ttcggcttcg gcgagaatga tttctttacg accccaagcg gctaaagaaa
     3961 tatcggcaac tttatagtcg ccaacggatg tcattgcagt catgacagac tcctatgg
//
LOCUS       DAOQMB010000203         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11192_length_4014_cov_4.468300, whole genome shotgun sequence.
ACCESSION   DAOQMB010000203 DAOQMB010000000
VERSION     DAOQMB010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11192_length_4014_cov_4.468300"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..984
                     /locus_tag="PLY05_07465"
     CDS             100..984
                     /locus_tag="PLY05_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011112293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNC03177.1"
                     /translation="MDNLTELYCLVDNFCQVFEPLWEQHLLTTGKKQRRRTASLSLSE
                     LITLVILFHQLRFRHFKTFYQEYVQVYLRREFPSLPSYNRCVELMPRCFSALFAFFQT
                     VKGQCTGINFIDSTCLAVCHNRRISRHRVFARYAGRGKNSVDWFYGFKLHLVINHRGE
                     VIDFRLTAGNVDDRKPVKGFCQKLFGLLVGDKGYVGAALSEELTQQGVQLITPVRRNM
                     TPPKRTAFERYLLQHRSLIETVNDELKNLCQIEHSRHRSLNNFLVNLMAGLVAYSLAP
                     KKPKLPIKVNYPFQSLAT"
     gene            1298..>4014
                     /locus_tag="PLY05_07470"
     CDS             1298..>4014
                     /locus_tag="PLY05_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HNC03178.1"
                     /translation="MSQSANSSINFDLSNINMWNGGDATGFDLANVITANTGLFEHFA
                     EDNLIGYKVDLSHHVQDTLAGIDYSYDYGLEVTAGLILKAKATLGELDLSYKIDAVND
                     LPTEVKSGDRFDIDTSKWTALSQLTSLGPSIDIQIGTTFTLNAYMRNLTMDFPAIDPV
                     SLGTLFDASVSFSHDFINTEDDLDLTYSLPSPTSLVSISGELPNGLAGTSGEPATTQN
                     ALPNVVSQAQQEGYLLEANLDLIELAKLFVPAGKAIPDVSKDLYIDAGSGNWIPKAYD
                     PDSPEGLPVEIDSDGDGSIDEDKNKNIITLNYLLLDAQLKGGFKAFQEFTFKPQAVEI
                     RMEMKVDGQVVEVQTGKLGDIFGFTAPEAGYGNIEITPYYTLKGQIENKTGVIADMRL
                     ELSAFDFGATFENVTIGGQTFNPDIKFDLFGEDKALIDVALPDGGLQSDPWIWYNKTF
                     DVNSATLVGDTVGLVYGDYFASSNVQSISYAGDAFDNEIKGHNNHDFLEGLGGNDTIR
                     GYSGMDLIFAGDGQDVVYAGADDDTVDGNAGNDFLYGGKGYDTLYGDAHNNDSETGDD
                     FLSGDEGNDTLYGGNGHDYLDGGTGYDELHGGAGDDLLTSNDGDALYGEAGNDTYHLG
                     RGNYVLGDTQALGESWENNRYVFHATGVSYENTRFYRDGDSLQVVFSDYGFNQLGSMT
                     IASYGNQKVAEGLFIYNTDGSPNNNNYVGGFNLATVWSVISSEDDIPEKSLAVAGRFS
                     VLPSEAGSIGIGAPRPLVSEGDDVIDGTTGDDVINALGGNDWINVRRGNDTVYGGEGN
                     DSISLMVDGNDVVDGGLGVDSLSLSLGELNHYGVAGIWWLGIDATGQTITPVESITAF
                     DTIDTYVKTAITNTLTVYGNYSFSYSPNIANVTFNAIENLSVSASTIAET"
BASE COUNT         1130 a          721 c          933 g         1230 t
ORIGIN      
        1 acctgagttc gggataagaa aaataaggaa cgtacctttg aaaagggata gagtttgggt
       61 aacgacactc aaaaccagcc cgaaacaaaa ggtacgctca tggataatct taccgaactt
      121 tactgtcttg ttgataattt ttgtcaagtt tttgaaccac tatgggaaca acacttactg
      181 accacaggaa aaaagcaacg acgtcgaacc gcctctttat cgctttctga attaatcacc
      241 ctcgttattt tgttccatca gttacgcttt cgacacttta agacctttta tcaagagtat
      301 gtgcaagtct atcttcgtcg agaatttccc tctttgccca gttataaccg ctgtgttgaa
      361 ttgatgccgc gttgtttctc cgctttattt gctttctttc aaaccgtcaa aggccaatgc
      421 acaggcatta actttattga ttctacctgt ttagctgtgt gtcataatcg ccgtatttct
      481 cgacatcggg tgttcgcacg ttatgcagga cgtggtaaaa acagtgtaga ctggttttat
      541 ggctttaaac tccatttggt gattaatcat cgtggcgaag tgattgattt tcgtttgacc
      601 gcagggaatg tggatgaccg taaaccggtg aaaggctttt gccaaaagct ctttggacta
      661 ttggtcggtg ataaaggcta tgtaggtgct gctttatctg aagaactaac tcagcaaggt
      721 gtacagttaa ttacccctgt acgtcgaaac atgactcccc cgaaacgaac cgcctttgag
      781 cgatatttac ttcaacatcg ctctttaatt gaaactgtca atgatgagtt aaagaatctg
      841 tgtcagattg agcattctcg acataggtct ttaaacaatt tcttagtcaa tttgatggca
      901 ggtttagtgg cttattctct agctcctaaa aagccaaaac tccccattaa ggtcaattat
      961 ccctttcaat cccttgcaac ataaggaaat taaaccgaac tcaggttaag ttgccatttt
     1021 tacctgctcg ttttgttcca tgtgcataca ctgaaaccaa cggccaaacg tttgatatta
     1081 actgactggc attacttcat ttgaagggct ttttgctttc tagactttct aaatccgcat
     1141 gcggaaacat tactttctgt ttaatcagga taatctatat gtgattgtct agcttttatc
     1201 atttaacctg tcataaacat tacatctaac ttgactctta tggtaatact cgtttaaaaa
     1261 ccattcagta ataactaaaa atttatggat ccttgccatg tcccagtctg ccaactcttc
     1321 aattaatttc gacctctcca atatcaatat gtggaacggt ggtgatgcca ctggttttga
     1381 tttagccaat gtgattactg ctaatacagg actatttgaa cattttgccg aagataactt
     1441 aattggctat aaagttgacc tttctcatca tgtgcaagac acattagcag ggattgatta
     1501 ttcctacgat tatggcctag aagtcaccgc aggtttaatt ttaaaagcca aagccacctt
     1561 aggcgaacta gatttaagct acaaaataga tgctgtgaat gacctgccaa ctgaggttaa
     1621 atcgggcgat cgctttgata tagacaccag taaatggaca gcactgtcgc aactcaccag
     1681 tctcggccct tctatagata ttcaaattgg tacaacgttt acattaaacg cttatatgcg
     1741 taatttaaca atggattttc ctgcgattga ccctgtgagt ttgggtacgt tatttgatgc
     1801 tagtgttagc tttagccatg actttatcaa taccgaagat gatttagatt tgacttattc
     1861 gctacctagc ccaacaagtt tagtcagcat cagtggtgaa ctacccaatg gtttagcagg
     1921 cacatcgggt gagccagcaa ccacgcaaaa tgctttgcct aatgtagtct ctcaggcgca
     1981 acaagagggc tatttattag aggctaacct cgatctaatt gaattagcca aattatttgt
     2041 accagcaggc aaagccattc ccgatgtgtc taaagattta tatattgatg cggggagtgg
     2101 caattggata ccaaaagcgt acgatccaga tagtcctgaa gggctaccag tagaaattga
     2161 cagtgacggt gatggcagca tagacgaaga caaaaacaaa aacattatca cgctcaatta
     2221 cttattatta gatgcccaat taaaaggtgg ttttaaagcc tttcaagagt ttacttttaa
     2281 gccacaggct gtcgaaattc gtatggaaat gaaagttgac ggccaagtgg tagaagttca
     2341 aacaggcaaa ctaggtgata tatttggttt tactgcgccc gaagcaggtt atgggaatat
     2401 tgaaattacg ccgtattaca cccttaaagg gcaaatagaa aataaaacag gcgtgattgc
     2461 tgatatgcgt ttagagctat cggcttttga ttttggcgca acttttgaaa atgtcaccat
     2521 tggtgggcaa acctttaacc ctgacattaa atttgattta tttggcgaag ataaggcgtt
     2581 aattgatgtc gcgctacccg atggcggttt gcagtctgat ccgtggattt ggtacaacaa
     2641 aacctttgat gtcaatagtg ctacgttggt gggagacacc gttggtttgg tgtatggtga
     2701 ttactttgca tcatcgaacg ttcaatcaat atcttatgca ggtgatgctt ttgataatga
     2761 aattaaaggc cacaacaacc atgatttttt agaaggctta ggcggtaacg ataccattcg
     2821 tggctattca ggaatggatt taatttttgc aggtgatggc caagatgtag tgtatgcagg
     2881 cgcagatgac gatacggtag atggtaatgc aggtaacgac tttttatatg gcggtaaagg
     2941 ctacgatacc ctatatggtg atgcccataa taatgatagc gaaacaggtg atgatttctt
     3001 atcgggtgat gaagggaacg atacgcttta tggggggaat ggtcacgact atttagacgg
     3061 tggtacaggt tacgatgagt tgcatggtgg tgcaggcgat gatttattaa ccagtaatga
     3121 cggtgatgcc ttatatggtg aggcaggtaa cgatacttac catttagggc gtggcaatta
     3181 tgtgttgggt gatacgcagg cgttgggtga gagttgggaa aacaaccgtt atgtgtttca
     3241 tgcgacaggt gtgagttacg agaatacgcg cttttatcgt gatggtgata gtttgcaggt
     3301 ggtgttttca gattatggct ttaatcagtt aggcagtatg acgattgcga gttatggcaa
     3361 tcaaaaggtc gccgaaggct tgtttattta taacaccgat gggtcgccca ataacaataa
     3421 ttatgtgggt gggtttaatt tggcgacagt gtggtcggtg atttcgtctg aggatgatat
     3481 tcctgagaag agtttggcgg ttgcagggcg gtttagtgtg ttgccgagtg aggcggggag
     3541 tattggcatt ggtgcaccga ggccgttggt gtcggaggga gatgatgtta ttgatggaac
     3601 aactggtgat gatgttatta atgctttagg tgggaatgat tggattaatg tgaggagagg
     3661 taacgacacg gtttatgggg gagaaggcaa cgacagtatt agtttaatgg tagatggcaa
     3721 tgatgttgta gatggaggtt taggggtaga tagtctgagt ttgagtttag gcgaattaaa
     3781 tcattatggc gtggctggta tttggtggct aggtatagat gctacaggac aaacgattac
     3841 gcctgttgag tcaattactg catttgacac aattgacacg tatgtcaaaa cagcgatcac
     3901 taatacctta acggtttacg gtaattacag tttttcttat tctcccaata ttgctaatgt
     3961 aactttcaat gctattgaaa atctcagtgt gagtgcaagc actattgctg aaac
//
LOCUS       DAOQMB010000204         3993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11288_length_3993_cov_15.244794, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000204 DAOQMB010000000
VERSION     DAOQMB010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_3993_cov_15.244794"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..450
                     /locus_tag="PLY05_07475"
     CDS             52..450
                     /locus_tag="PLY05_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03179.1"
                     /translation="MLQQNQPFSWGWILASVGIFVITQLVLAYIVGQVLLGARIPHGL
                     QQMVQGFLMLLSYFVGGFLIGVVSPKIRIIEPALGAFASIFLVVIFSFLMPNSYVHFE
                     WLRLLIGGVIAFGLAMYGAVLGEKLTKQLP"
     gene            complement(495..1034)
                     /locus_tag="PLY05_07480"
     CDS             complement(495..1034)
                     /locus_tag="PLY05_07480"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HNC03180.1"
                     /translation="MTTTTPLATGYTEIAGVKLANGKSIEGQNLVLNGAGLRTLALFK
                     VYVAALYLGQKNNNAEEIINSAAPQCIELVLKRDVDAKTISGSFRDALKQNLSKEELQ
                     ALEARLDELEGAINNIRQIKEGDRLCIDFAGNGLTRIWFNDALKETISGESLSSALLK
                     IWLGKKPVQDNLKKALLGA"
     gene            1282..1938
                     /locus_tag="PLY05_07485"
     CDS             1282..1938
                     /locus_tag="PLY05_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004948903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNC03181.1"
                     /translation="MTPLAQQLEQQLQRTLATATITAQSLPDVDDLSLYLLNPDYPRT
                     PMSSEQMQAIWQEPAYWIFCWASGLAMAKWLKENPDYVRGKRVLDFGAGSGVVAIAAK
                     QAGAAEVVACDIDPLALLACKANAELNGVELSYSQNFYQLTERYDVLLAADVLYDASN
                     RHFLDEFLARAQQVLVADSRVKNFQHPHYQKLAEQGATTWPDLDEFDEFRVVRFYGSC
                     "
     assembly_gap    1939..2038
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2039..2791)
                     /locus_tag="PLY05_07490"
     CDS             complement(2039..2791)
                     /locus_tag="PLY05_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03182.1"
                     /translation="MDWNQVLSYAAKATGFLKKAVDTLAEERDLHLPESVLNTALSGL
                     ASRSKHLHLLNFELHEDWFDVDLVYEHQGFEFGIKASFDVEKITFDHHKQSLSLRERR
                     PIEFTPRRFKSSWQQLGFNLWSWWLKQVKKTTPLLHTLNNIEGMSAKNGMYRMDFSPH
                     IRRKPAFVATLYALDIKEIRILDNEVYLRGSADLKSLNLLNELYKLLPQQDLTDAKNS
                     PTHNVTAISNKPLTEEEADLIIDKILASKTKE"
     gene            2863..3099
                     /locus_tag="PLY05_07495"
     CDS             2863..3099
                     /locus_tag="PLY05_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03183.1"
                     /translation="MSDLLAQKAHLEARYAELNQRLQAIRQDYANGLDADSEERAQQL
                     ENEEVLAEIMRVAQEDLAKVAQQLLEVSALLAKA"
     gene            3099..>3993
                     /locus_tag="PLY05_07500"
     CDS             3099..>3993
                     /locus_tag="PLY05_07500"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNC03184.1"
                     /translation="MATPLKEQLADFAIHTIADVLQKIYPNFASQSFIAQALQGLETL
                     ELKQRVQHIIVVLANYLPQDFEQTAAILQEIPEHWPSQINQAYSAFVAWPLIDYVAVY
                     GLAQPQIALPTLAKLTPLFTAEFAIRPFLQHHFEITYGYMQQWAQHEHEHIRRLASEG
                     LRSRLPWGQRVAKLLAEPQWAITILQQLKDDSSDYVQRSVANNLNDLSKDYPQQVIEL
                     AQQWLKDTPTAQRQWIIKHGTRSLIKAGHADALGLLGYKTQIAIENIKFKVDKTHVNM
                     GESVSLHLSFDLPFAQALVIDY"
BASE COUNT         1140 a          710 c          796 g         1247 t
ORIGIN      
        1 tttcgatgaa atgcgtaaat cctgattata ttttttgaag gaaaagacaa gatgttgcaa
       61 caaaatcagc cattttcatg gggatggata ttagccagtg tggggatttt tgtcattaca
      121 caactcgttc ttgcttatat tgtcgggcaa gtattattag gtgcgcgtat tccacatggt
      181 ctgcaacaaa tggtacaagg ctttttgatg ttattaagct attttgttgg cggttttttg
      241 attggagtgg tgtcgcccaa aattagaatt attgagcctg cattgggtgc gtttgcgtca
      301 atttttttag tggtcatctt ttcgtttttg atgcccaata gttatgtgca ttttgaatgg
      361 ttacgattgt taattggtgg tgtaattgct tttggtttag caatgtatgg tgcagtgttg
      421 ggcgaaaagt taaccaaaca actgccataa atcgctacaa agaaggtctg ctagagacct
      481 tcttgtactg atttttaagc acctaataac gcttttttaa gattgtcttg aacaggcttt
      541 ttacctaacc aaatttttaa taacgcactt gataaacttt cgccagaaat agtctctttt
      601 aaagcgtcat taaaccaaat acgagttaag ccattacctg caaagtcgat acacaaacga
      661 tcgccttctt taatttgacg aatgttatta atggctcctt ctaactcatc aagacgagct
      721 tccaaagctt gtaattcctc tttggataaa ttttgtttta aagcatcacg aaaactaccc
      781 gaaatagttt tggcatcgac atcgcgtttt aaaactaatt cgatacattg tggtgctgcg
      841 ctattgatga tttcttcggc attattattt ttttggccaa gatataaagc ggcaacatag
      901 actttaaaca gtgctaaggt gcgtaaacct gcaccattga ggactaagtt ttgtccttcg
      961 attgatttac cattagctaa cttaacgcca gcgatttctg tgtaccctgt tgctaaagga
     1021 gttgtggtgg tcatgatcaa aatcctctaa ataagtgctt atgatgttca gtttttaaaa
     1081 ctcatactta gttgttataa gcacggctat tatatagata tatatatata tgtaacagta
     1141 gtcatctaaa atgaatggta gtcataagga ttttcaaata agcatcgtgt gtttttggtg
     1201 agagattgtt gaggagtgat ggtgaggaaa atcgatcaaa aatgttataa ttggctttca
     1261 acccctgttt tattagccat tatgacccct ttagcccaac aacttgagca acaattacaa
     1321 cgtactttag ccacggcgac aattacggct caatccctgc ccgatgtcga tgatttatcc
     1381 ttatatttat taaatcctga ctatcctcga acaccaatga gtagtgagca aatgcaagcc
     1441 atttggcaag agccagccta ttggattttt tgttgggcat caggtttagc aatggctaaa
     1501 tggctgaaag aaaatcctga ttatgtgcgt ggtaaacggg tgttggattt tggtgcgggt
     1561 tctggtgttg tagccattgc cgctaaacaa gcaggggcag ccgaagtggt cgcctgtgat
     1621 attgatcctt tagccttatt agcctgtaaa gccaatgctg agttaaatgg tgttgaatta
     1681 agttatagcc aaaattttta tcaattgacg gaacgttatg acgtattgtt agccgcagac
     1741 gtattatatg acgcgagcaa tcgacatttt ttggatgagt ttttagcacg tgcacaacag
     1801 gtattggttg ccgattcgcg ggtgaaaaac tttcagcatc ctcactatca aaaactggca
     1861 gagcagggag caactacatg gccagattta gacgagtttg acgaatttag agtagtgcgt
     1921 ttttatggtt cttgttgann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     2041 attccttggt cttagacgcc aagattttat caataattaa atcagcctct tcttcggtta
     2101 atggtttatt gctaattgcc gtaacattat gtgttgggga gttttttgca tcagttaaat
     2161 cttgttgtgg cagcaattta tacaactcat taagtagatt cagcgacttc aaatcggcag
     2221 agccacgcaa atacacttca ttatccaaaa tacgaatttc tttaatatct aaagcataga
     2281 gtgtggccac aaaagcaggt ttgcggcgaa tatgcggcga aaagtccatg cgatacatac
     2341 catttttcgc actcatgcct tcaatattat tgagtgtatg cagcagaggt gtggtctttt
     2401 taacttgttt tagccaccaa gaccataaat taaagccaag ttgctgccat gatgatttaa
     2461 aacgacgtgg tgtaaattca attgggcgac gttcgcgtaa acttaaactt tgtttatgat
     2521 ggtcaaaagt aattttttcg acatcaaagc tcgctttgat accaaattca aaaccttgat
     2581 gttcatagac taaatcaaca tcaaaccaat cttcatgcaa ttcaaaattt aataaatgta
     2641 aatgtttgga gcgcgaagcc aagcctgata atgcggtgtt taacacagat tcaggcaaat
     2701 gcaaatcacg ctcttcggct aaggtatcaa ccgctttttt aagaaaacct gtcgcttttg
     2761 cagcgtaact taatacttga ttccaatcca tattttacct ttattattca tcgcaaacat
     2821 gtatataatc aaaaatagcc ttaattttta tggggaaatt ctatgtccga tttattagct
     2881 caaaaagccc atttagaagc acgttatgcc gaattaaacc agcgtttgca agctattcgc
     2941 caagattatg ccaatggttt ggatgctgat agtgaagaac gcgcccaaca actcgaaaac
     3001 gaagaggttt tagctgaaat catgcgtgtt gctcaagagg atttagcaaa agtagcacaa
     3061 caattgcttg aagtttcggc attattggct aaagcgtaat ggccacacca ttaaaagagc
     3121 aattagcaga ctttgctata cacactatag ccgatgtttt gcaaaaaatt tatccaaatt
     3181 ttgctagtca gtcttttatt gctcaagctt tacaaggttt agagacttta gagctaaaac
     3241 aacgggtgca acatattatt gttgttttag ctaactacct tccgcaagat tttgagcaaa
     3301 cagccgccat tttgcaggag attcctgagc attggccaag ccaaattaat caagcgtata
     3361 gtgcgtttgt cgcttggcca ttaattgatt atgtggcggt atatggttta gcgcaaccac
     3421 aaattgcctt gccaacctta gccaaattaa cgcctttatt tactgccgaa tttgccattc
     3481 ggcctttttt acaacatcat tttgaaatca cttatggtta tatgcagcaa tgggcgcaac
     3541 atgagcatga gcatattcgg cgtttagcct ctgaggggct acgttcacgt ttgccgtggg
     3601 gacagcgtgt ggctaagtta ttggctgaac cacaatgggc aatcaccatt ttgcagcagc
     3661 tcaaagatga ttcgagtgat tatgtgcaac gttcagtggc caataactta aatgatttaa
     3721 gtaaagatta cccacaacaa gtcattgagt tagctcaaca atggcttaaa gatacgccaa
     3781 ctgctcaacg gcaatggatt attaaacatg gtacacgcag cttgattaaa gcggggcatg
     3841 cggatgcttt gggtttattg ggttataaaa cacaaatagc cattgaaaac attaagttta
     3901 aagtcgataa aacacacgtt aatatgggcg agtcggtgag cttacattta tcttttgact
     3961 tgccatttgc tcaggcttta gtgattgatt atg
//
LOCUS       DAOQMB010000205         3984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11329_length_3984_cov_5.401629, whole genome shotgun sequence.
ACCESSION   DAOQMB010000205 DAOQMB010000000
VERSION     DAOQMB010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11329_length_3984_cov_5.401629"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(420..1511)
                     /locus_tag="PLY05_07505"
     CDS             complement(420..1511)
                     /locus_tag="PLY05_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03185.1"
                     /translation="MRPFLIVLICSLFYVSQTIAEPLNSIEDINILSGDEHDITYYIT
                     IKEVNNHWDINSINKDFMTPDDYKSVEILGVSEDLKTIKPAFQKYYGGKKGSFACSKG
                     GEEYTRQRAPDVPNVYNPCESKLTSVNKTKAIVSNTFNTAFRLGLNIATGQVVYSVDT
                     DKAKIKEIIQETQLLEKLKNYIHEQKLASYREEFKTAYSSKKLQSIIEKYQGNDPENL
                     IPSAIERKKTLELKEADEQQKMLEKRKVELEHQAAIDKKKAEIAYQEEVEHRKFIAEQ
                     ERERQVRYKQLMSDVKSFQATIKMGIQTNCGPVLETKGTLIKVYFPVKDYGNEHWIKK
                     STLYPSIYPCSFANGRYIEPNVYGAYQEN"
     gene            complement(1508..>3984)
                     /locus_tag="PLY05_07510"
     CDS             complement(1508..>3984)
                     /locus_tag="PLY05_07510"
                     /inference="COORDINATES: protein motif:HMM:NF019530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HNC03186.1"
                     /translation="SVSTFSSWTTKTFETVFSLPNGMRYSQHGPLFGQSLVQDMLAYR
                     PNNANLTNSLQSFWQNCVFYDIALGFYTFDELSAQSDILSFLKTRTAMTRGFYNEAGG
                     AKTFVDCRTGATTLEQDLLVEQQDFKSRSGNKANVTMKSTDAMASAGWTAMPVAFAYL
                     SNVSTTASKLLIQSAMMNSMDTGLSSMAGNVDADAAIQGYALARAERERETTFGVMGA
                     MAAEMLPLLKNLMEGLMYAVFPLVGLAILFPNGWRAFGYYARMLIWIGLWPVAFALLH
                     YAMTYFGSFAVQKAAAHYDLLGNFSESKYNMYSQMGIMDVMKKYEAIAGYMMTMIPMV
                     TYVMVAQGGAMMAGMLGRVMDGYAKPAESAAMEASAGNFSVGNTNFDNQIAFQHMSAP
                     SQTAGFGQINQGLRQQMYSEGAVTTTFAQSNLPITANTVQNIGSRLQTEHAQAVERSH
                     EASIAQLNATQDVIRQLSSQGSSFNQTHSKGTSTGSTESTSQQNMTNHVTQAAKEWVN
                     QNSHSDETLRRLSAEWGVSGGVNSQNQLIGKLVGAVTGAHAEVSGKVGGGVAGTTSNS
                     DVQQLAQRFAASETFQHTLTKLASDQIANMNSNTTTGSTEQREALEKSVSKMHSATEQ
                     QAKTLRESERTSKAEADFRENSASITTPMVGAINNIARQRLTDEGIYEAVAALERGDL
                     KDDNLQRLQEVIKDVVTGQVQTSITANVAQDIRADYQQDKVSVPHQAVKPNHDTHDKV
                     QGVANQVSTAQAASQQSINSSNTDINNKQNEIAADGQHTRQTITDEVGVSQPESRHAD
                     SGMTFIANQFNDTLERTRNIGRGENK"
BASE COUNT         1050 a          890 c          712 g         1332 t
ORIGIN      
        1 ttagggtcaa cagcgcgagc tgattaactc ttccctgatt actaagggat taagaccttt
       61 ttttactgtc ataattgaca gataaagacg ttaaaactct tccctgatta ctaagggatt
      121 aagacttatc gtggttgcca attcgcaacc actagtttga ttaactcttc cctgattact
      181 tgtcaacgcc aatttaaaat tgaccctctt agatcgttgt attaagtata aaatttaagt
      241 aagtttaggt tttaagtatt taaactcggt gtattgccaa ctaaggtagc ctacgcgccc
      301 cactatatgg ttggggcgcg tttgctgttt tttataaaac tcttcattta aagcgttttt
      361 taatgttagc ccatgccaac atcacacagt aaaacatatt ctcccttagc atattatgct
      421 taattttctt ggtatgcccc atatacattt ggctcaatat atcgcccatt agcaaaagaa
      481 catgggtaaa ttgaggggta caaggtactt tttttaatcc aatgctcgtt tccataatct
      541 ttgacaggaa aatacacttt tattagcgtt cctttagttt ccaaaacagg gccacaattg
      601 gtttgaatac ccatttttat tgttgcttga aagcttttta cgtcactcat taattgtttg
      661 tatctaactt gcctttctct ctcttgttca gcaatgaatt tacgatgctc gacttcttcc
      721 tgataagcaa tctcagcttt tttcttatca atagccgctt gatgctctag ctcaactttg
      781 cgcttttcta acattttttg ctgctcgtca gcttccttta attcaagtgt ttttttacgt
      841 tctatcgcac tgggtattaa attttctgga tcattacctt gatatttttc aataatactt
      901 tgcaactttt tagaactata agctgtttta aattcttctc ggtaagaagc aagcttttgc
      961 tcatggatat agttttttaa tttctccaac agttgtgttt cttgaatgat ctctttgatt
     1021 ttagctttat ctgtatcaac gctatatacc acttgcccag tggcaatatt taagcctagt
     1081 cgaaatgccg tattgaatgt attgctaaca atagccttgg tcttattcac agaagttagc
     1141 ttactctcgc aaggattata tacattagga acatcaggtg ctctttgcct cgtatattct
     1201 tctccacctt ttgaacatgc aaaactgcct ttttttccac cataatattt ctgaaatgct
     1261 ggtttaattg tctttaaatc ttctgaaacg cctaatattt caacagattt ataatcatca
     1321 ggggtcataa agtctttatt tatactgttg atgtcccaat gattattaac ttcttttatt
     1381 gttatatagt aggtaatatc atgctcatca ccacttagaa tattaatatc ttcaatgcta
     1441 ttcaaaggct cagcgatggt ttggctcaca taaaatagcg aacaaatcaa aactattaga
     1501 aacggtctca tttattttct cctctaccga tattgcgcgt tctctctaaa gtgtcattaa
     1561 actgattggc aataaacgtc atcccagaat cagcatgacg tgattcaggt tgagaaacgc
     1621 ctacttcatc agtaatggtt tgtcgtgtgt gttggccatc tgctgcaatt tcgttctgct
     1681 tgttgtttat gtctgtatta gagctattaa tgctttgttg actggctgcc tgagctgtag
     1741 agacttggtt tgcaacacct tgtaccttgt cgtgtgtatc atgattgggt ttgactgctt
     1801 gatgagggac agaaacttta tcttgttgat aatcggcacg aatatcctgt gccacattag
     1861 cggtgatgct agtttgcacc tgtcctgtca caacatcttt aataacctct tgtaatcttt
     1921 ggaggttatc atcttttaaa tcaccacgct caagggcagc aactgcctca taaatacctt
     1981 catctgttaa gcgttgtcgt gcaatgttat taatcgcacc aaccatcgga gttgtaatag
     2041 atgctgaatt ttctctaaaa tcagcctctg ccttagaggt gcgctcagac tctctaagcg
     2101 ttttagcttg ttgttctgtt gctgagtgca tcttactgac tgatttttca agagcctcac
     2161 gctgctcagt gctaccagtc gtggtattac tattcatatt agcaatttga tcactcgcca
     2221 atttagtcag cgtatgttgg aatgtctcac ttgccgcaaa acgttgtgcc aattgctgta
     2281 catcactatt tgatgttgtg cctgctactc ccccaccgac tttaccggat acctccgcat
     2341 gtgcgcctgt taccgcacca acgagcttcc caatcaattg attttgtgag tttacgccac
     2401 ccgatacacc ccattctgct gacagtctgc gtagtgtttc atcagagtga ctattttggt
     2461 taacccattc tttggctgcc tgagtcacat gattcgtcat attttgctga cttgtactct
     2521 ctgtactacc tgtacttgtg cctttactat gcgtttggtt gaagctagaa ccctgactac
     2581 ttaactgacg aataacatct tgagtcgcgt ttaattgagc aatgctggcc tcatgactac
     2641 gctcgacagc ttgagcatgc tcagtttgca aacgtgaacc tatgttttga acggtattcg
     2701 ccgtaatagg taaattactt tgagcaaagg ttgtggtaac tgcaccttcc gaatacatct
     2761 gttgtctgag tccctgattg atttgaccaa atccagccgt ttgacttggc gcactcatat
     2821 gttgaaatgc tatttgattg tcaaaattag tattaccaac actaaagtta cctgccgatg
     2881 cctccatagc agccgactct gcgggtttgg catagccatc catcacacgg cctaacatac
     2941 ccgccatcat tgcaccacct tgagcaacca tcacataagt caccattgga atcatcgtca
     3001 tcatataacc tgcaatggct tcatattttt tcatcacatc catgatgccc atttgactat
     3061 acatattgta ttttgactca ctaaaattac ccaataaatc ataatgagca gccgcttttt
     3121 gtaccgcaaa gctcccaaaa taagtcatag cataatgtaa taaggcaaat gcgactggcc
     3181 acaatccaat ccatatcaac atccttgcat aatagccaaa agctctccaa ccattaggaa
     3241 acaagatagc tagtcccacc aaaggaaaca ccgcatacat taaaccttcc atgagatttt
     3301 ttaggagtgg cagcatttct gcggccattg cccccatgac accaaacgtg gtttcgcgct
     3361 cacgctcggc tcttgctagt gcatatcctt gaatggctgc atccgcgtca acattacctg
     3421 ccatagacga taagcctgtg tccattgagt tcatcattgc actttgtatc agtagctttg
     3481 aagcggtggt tgacacatta gaaagataag caaatgcaac gggcattgct gtccaacctg
     3541 cactcgccat ggcatcagtt gatttcatgg tcacattggc tttattacca ctgcggcttt
     3601 taaaatcctg ttgttcgact aacaagtctt gctctagagt tgttgcgcct gttctgcaat
     3661 caacaaacgt tttagctcct cccgcctcat tataaaagcc ccttgtcatg gcggttcgtg
     3721 tttttaaaaa gcttaaaata tcactctgag cggagagttc atcgaaggta taaaaaccca
     3781 acgcaatgtc ataaaaaaca caattttgcc aaaagctttg caggctgttg gtaagattgg
     3841 cattatttgg acgataagca agcatatctt gcacgagcga ttgcccaaat aaagggccat
     3901 gttgcgaata cctcatgcca ttaggcaacg agaatactgt ctcgaaggtt tttgttgtcc
     3961 acgaactaaa ggtactgacg ctac
//
LOCUS       DAOQMB010000206         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11579_length_3937_cov_17.262751, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000206 DAOQMB010000000
VERSION     DAOQMB010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_3937_cov_17.262751"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..789
                     /locus_tag="PLY05_07515"
     CDS             181..789
                     /locus_tag="PLY05_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03187.1"
                     /translation="MFKYSSKIIITLLTCSLFTACSSTPTNKSLVKEQLSIEKQRRNA
                     AQDLAEQTLDTMPSWAMTAPRPDNTGVYAVGVAESDKVQIAIKKAQLQAQYGLAKIFN
                     QELAGSEQMMQQDAGAGSSSSYQSLIQSLVNYVPVVGFEVVKQEVKAVNGEFQAYTLL
                     KLPYAEFNKALQSQKQQAQNTDMKQAFADLERRLQQHQLQKK"
     gene            808..>1333
                     /locus_tag="PLY05_07520"
     CDS             808..>1333
                     /locus_tag="PLY05_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03188.1"
                     /translation="MMRSCLSLSISCCLLLCATSGWAWNKDYQTEYVLGDSDSRLTAK
                     QQALKQIQLQAMQEAGTYIQGQTQLLNDKLDEKINQVSAVIVKLQVLTEHFELTATGQ
                     QKLRLMARANVDESVLQQRVQALQQETNQAKQQQPILTAIQQEQQKLMLDNQLLRRQL
                     QVLSQQGQLPTNISP"
     assembly_gap    1334..1433
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1579..2343)
                     /gene="hisF"
                     /locus_tag="PLY05_07525"
     CDS             complement(1579..2343)
                     /gene="hisF"
                     /locus_tag="PLY05_07525"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNC03189.1"
                     /translation="MTLAKRIIPCLDVDKGRVVKGVQFLDIRDAGDPVEVAKRYDQQG
                     ADEITFLDITASHEGRDTTLKTVERMASEVFVPLTVGGGIRKLEDIRNLLNAGADKVS
                     INSAAVTNPDFVREAAERFGSQCIVVAIDAKKVADGRWEIFTHGGRKPTGINAVEWAV
                     KMANYGAGEILLTSMDADGTKNGYDLGVVRAVSDAVNVPVIASGGVGNLQHLADGILQ
                     GRADAVLAASIFHFGEYTVPQAKAFLREQGIVMREI"
     gene            complement(2396..3127)
                     /gene="hisA"
                     /locus_tag="PLY05_07530"
     CDS             complement(2396..3127)
                     /gene="hisA"
                     /locus_tag="PLY05_07530"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005087083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNC03190.1"
                     /translation="MLIIPAIDLKDGKCVRLQQGRMEDNTVFSDDPVGMATHWVEQGA
                     RRLHLVDLNGAFAGTPIHSEVVKAIAKKHPALPIQIGGGIRSLETIESYIKAGVTWVI
                     IGTKAVKEPQFVVDACREFHGRIIVGIDAKDGWVATDGWAEVSQVKAEDLAKNFAQAG
                     VSAIVYTDIARDGMMQGVNVQATAALARASGLPVIASGGVTNLDDIRRLKTVAHDGVI
                     GAITGRAIYEGTINVAEAQQLADAE"
     gene            complement(3237..3869)
                     /gene="hisH"
                     /locus_tag="PLY05_07535"
     CDS             complement(3237..3869)
                     /gene="hisH"
                     /locus_tag="PLY05_07535"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNC03191.1"
                     /translation="MTTVAVLDYGMGNLHSVAKALEHVGASKVIITHDPALVRAADRV
                     VFPGVGAMRDCMAGMKAVGVDAEVREAASQKPVLAICVGMQALMEHSEENGGVDCLAM
                     FNGKVKFFGNDLVENGQRLKVPHMGWNEVNHTIDHALWRDIPQNSRFYFVHSFYVDVA
                     DSQLIAGRSHYGLDFTTAISHDNLFAVQFHPEKSQKMGLQLLKNFVEWRV"
BASE COUNT         1172 a          872 c          774 g         1019 t
ORIGIN      
        1 agcggttttc tgatgtacta gccaacaatt tttgttggct atacctacat aataagtagt
       61 ttttatgtgg gaaatatatt tttatatatt ttaaattatt gattttaaaa gtattaaaag
      121 ttggcatggt atctgtaatg attaagttga gtttataaca tcaacaacaa ggacattacc
      181 atgtttaagt attcttcaaa aattatcatc actcttttaa cttgtagctt gtttacggct
      241 tgtagttcca ctcccaccaa caaaagtttg gtcaaagagc agttaagcat cgaaaaacaa
      301 cgtcgaaatg ccgctcaaga ccttgccgag caaaccttag ataccatgcc ttcatgggcg
      361 atgactgcgc cacgtcctga taatacgggc gtatatgcgg tgggtgtggc cgaaagtgat
      421 aaagtgcaaa ttgcgattaa aaaagcccaa ctgcaagcac agtacggttt ggcaaaaatt
      481 tttaatcaag agttggcagg tagcgaacaa atgatgcaac aagatgcggg tgcagggtca
      541 agtagtagtt atcagagttt gattcagtct ttagtgaatt atgtacccgt tgttggtttt
      601 gaagtggtaa aacaagaagt taaagccgtc aatggagagt ttcaggctta taccctgtta
      661 aaactacctt atgcagagtt taataaagcc ttacaaagcc aaaaacaaca agcacaaaat
      721 accgatatga aacaggcttt tgcggatttg gagcgtcgtt tacaacaaca tcaattacaa
      781 aaaaaataac tctaagttga ggttgttatg atgaggtcgt gtttgtcatt gagcataagt
      841 tgttgtttat tgctttgtgc cacatcagga tgggcatgga ataaagacta tcaaaccgaa
      901 tatgtattgg gtgatagcga tagtcgatta actgccaaac aacaggcttt aaagcaaatt
      961 caattacaag ccatgcaaga ggcaggcacg tatatacaag gtcaaacaca attgctcaat
     1021 gacaagttgg atgaaaaaat caaccaagtc agtgcggtaa ttgttaaatt acaggtatta
     1081 accgagcact ttgagttaac cgcaacagga cagcaaaagt tgcggttaat ggctcgtgct
     1141 aatgttgatg agtccgtatt acaacagcga gttcaagcct tgcaacagga gactaatcag
     1201 gcaaaacaac aacaaccaat tttaacggcg attcaacaag agcaacaaaa gctaatgttg
     1261 gataatcaat tattacgtcg acagctacag gtattaagtc aacaagggca attacccaca
     1321 aatatttcgc ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntttattg
     1441 tgtgcggtta agttgcctaa acaatgcagt ccattagaga tagagtcttt gggtaaagat
     1501 aatggtcaga tgtcggttgc taaaagagtg attgattcat attagtagcg aagtttagtt
     1561 aacttcgctc tttcgggttt aaatctctct catcacaatc ccttgctcac gcaaaaacgc
     1621 ttttgcttga gggacggtgt attcgccaaa gtggaaaata ctggcagcta acaccgcatc
     1681 agcacgccct tgcaaaatac catcggccaa atgctgcaaa ttacccacac cacctgatgc
     1741 aatcacaggc acattcaccg catcactcac tgcacgtaca acacctaagt catagccatt
     1801 tttagtgcca tctgcatcca tactggttaa taaaatttca cccgccccat agttggccat
     1861 ttttaccgcc cattcaacgg cattaatgcc tgtcggttta cggccaccgt gggtaaaaat
     1921 ttcccaacgg ccatcggcaa cttttttggc atcaatggca accacaatac attgtgagcc
     1981 aaaacgctca gccgcttcac ggacaaaatc agggttagtc accgctgcgg agttaatact
     2041 cactttatca gcacctgcat tgagtaaatt gcgaatatct tccaacttgc gaatgccacc
     2101 acccactgtt aacggcacaa acacttcgga agccatgcgt tcaacggttt ttaaagtcgt
     2161 gtcacggcct tcatggctgg cggtaatatc caaaaaggta atttcgtctg caccttgttg
     2221 gtcatagcgt ttggccactt caacagggtc accagcatca cgaatatcta aaaactggac
     2281 acctttgacc acacggcctt tatcaacatc taaacaagga ataatacgtt tggcgagagt
     2341 cataagcata cctactccct ctccctatgg gagagggctg gggagagggt taaagttatt
     2401 cagcatccgc taattgttgg gcttcagcaa cgttaatcgt accttcataa atagcacgac
     2461 ctgtaattgc accaatcacg ccatcatgcg caacggtttt taagcggcga atatcatcta
     2521 agttagtcac cccacctgat gcaatgacag gtaaaccact ggcgcgtgct aaagccgcag
     2581 ttgcttgtac gttaacacct tgcatcatgc catcacgagc aatatcggtg taaacaatgg
     2641 cgctaacacc tgcctgagca aagtttttag cgaggtcttc tgcttttact tgagaaactt
     2701 cagcccaacc atccgttgcc acccaaccat ctttggcatc aatgccaaca atgatacggc
     2761 catgaaactc acgacaagca tcaacaacaa attgaggctc tttaacggct ttagtgccaa
     2821 taatgaccca cgtaacaccc gctttaatat aagactcaat cgtttctaat gagcgaatac
     2881 ccccaccaat ttgaattggt agtgcaggat gttttttagc aatggctttc accacttcac
     2941 tgtgaatggg tgtgcctgca aaagcaccat ttaaatcaac gaggtgcaaa cgacgtgcgc
     3001 cttgttctac ccaatgtgtg gccatgccaa caggatcatc ggaaaacacg gtgttatctt
     3061 ccatacggcc ttgttgcaaa cgtacacact taccatcttt aaggtcaatt gcagggataa
     3121 ttaacataaa attctcaatt taatgttggc ggtggttaat ggcttcgact ccgctcagcc
     3181 aacttcgttc cctgagcgga gtcgaataag ggcgttctct gagcggagtc gaagagctaa
     3241 acacgccatt caacaaagtt ttttaacaac tgtaagccca ttttttggct tttttcaggg
     3301 tgaaactgta cggcaaataa gttgtcgtgg ctaatggcgg tggtaaagtc caaaccataa
     3361 tgactacggc cagcaattaa ttggctatca gcaacgtcga cataaaaact atgaacaaag
     3421 taaaaacggc tgttttgtgg aatatctcgc cataaggcat gatcaatagt atggtttact
     3481 tcattccaac ccatgtgtgg cacttttaag cgttggccat tttcaaccaa atcattgcca
     3541 aaaaatttaa ccttaccgtt aaacatcgcc aaacaatcaa cgccgccatt ttcttcggag
     3601 tgttccatta aggcttgcat acccacacaa atggctaaca caggtttttg gctggctgct
     3661 tcacgtacct cagcatcaac accaaccgct ttcatgcctg ccatacaatc acgcattgct
     3721 cccacaccag gaaacaccac acggtcagcc gcacgcacta aagcagggtc atgggtaata
     3781 atgactttac tcgcgccaac gtgttctaag gctttggcga cagagtgcaa attacccatg
     3841 ccataatcta aaactgcaac ggtagtcatt ataagctacc tttggttgag ggcatttgac
     3901 cagccatacg tgggtctaac tcagcagcca tacgcaa
//
LOCUS       DAOQMB010000207         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11580_length_3937_cov_12.767388, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000207 DAOQMB010000000
VERSION     DAOQMB010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11580_length_3937_cov_12.767388"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLY05_07540"
     CDS             complement(<1..194)
                     /locus_tag="PLY05_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNC03192.1"
                     /translation="MHQTILDNLSRIEEQYQVTILWACESGSRAWGFPSPDSDYDVRF
                     IYAQKPSSYIRVFDKRDVIE"
     gene            400..711
                     /locus_tag="PLY05_07545"
     CDS             400..711
                     /locus_tag="PLY05_07545"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNC03193.1"
                     /translation="MMKKLVTFMAVVALNGCAVVASPVGNGALFTATKGPVTATNLDS
                     ASKSGEACSQNLLGFIVSGDASIATAKKAGNISKVATVDHSSVAFLGLYSQFCTQVTG
                     E"
     gene            complement(795..2165)
                     /gene="purB"
                     /locus_tag="PLY05_07550"
     CDS             complement(795..2165)
                     /gene="purB"
                     /locus_tag="PLY05_07550"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNC03194.1"
                     /translation="MLSTLTALSPVDGRYASKADSLRPWFSEFGLIRARVTVEVRWLQ
                     QLSKHPQISEVPEFSPQTYMALEEIVSLFDEEDAQWIKNKERITNHDVKAVEYFLKDK
                     ASANPELAAVSEFIHFACTSEDINNLSHGLMLKAGRDIALEQMTAVYEAIRQLAHRYA
                     DLPMLSRTHGQTASPTTLGKEMANVAYRLGRQIQQFKAVEILGKINGAVGNYNAHLSA
                     YPDVDWAENAKQFIEGLGLNFNPYTTQIEPHDYIAELFDAVKRFNTILIDFNRDVWGY
                     ISLGYFKQRLKEGEVGSSTMPHKVNPIDFENSEGNLGIANATLAHLGEKLPISRWQRD
                     LTDSTVLRNLGVGLAHSLIAYEAALKGIGKLEVNPNRLAEDLDNAWEVLAEPIQTVMR
                     RFGISEPYEKLKAFTRGKVITAADIQSFLNSEKMASLPAEAKAALAALTPASYIGNAA
                     QQAKAV"
     assembly_gap    2367..2466
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2467..3612
                     /locus_tag="PLY05_07555"
     CDS             <2467..3612
                     /locus_tag="PLY05_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HNC03195.1"
                     /translation="NTPELGLMGVTEPELHGATRNPWNLQRTSGGSSGGAAAAVAAGI
                     VPMAAGGDGGGSLRIPASCCGLVGFKPSRGRNPYGPAMAEPWFGQVQEGVISRSVRDT
                     AAMLDATHGRDVGALYDAPTVKGTFLAAVSKSPKRLKIAYSQEALLSDALLDAECLRG
                     LKYTVDLLSSLGHEVVEATPPIDKRQLADGYLLRIAAATAGDVFATEQIIGRPVELED
                     FETETWSLARLGQSFSAYDLDNANRMIYQQVRIFETWMQDYDVFLTPTLAAPPIALGG
                     FRLQGVEKLLSPIAKRFKLGGLVHYLPILQHLAQQNFQWVVSTPVMNMTGNPSVSLPL
                     HWSQDNLPIGMMFTARYGDEMTLFKLAGQLEQAKPWFNLRPAIFAGS"
     gene            3616..>3937
                     /locus_tag="PLY05_07560"
     CDS             3616..>3937
                     /locus_tag="PLY05_07560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNC03196.1"
                     /translation="MKKWWLIMCSTWWLTACQSVSYYGQSIKGQWQILANRQPLEQVI
                     KHPQSSPALVKQLQYIQHIRDFSQELSLPTKGQYDTYVDIKRSAAMWSVAATPELSLT
                     AKTWC"
BASE COUNT         1111 a          743 c          828 g         1155 t
ORIGIN      
        1 tcttcaatca catcacgttt atcaaacacc cgaatataac tactaggttt ttgagcatag
       61 ataaaacgta catcataatc actatcaggt gagggaaaac cccatgcgcg gctacccgat
      121 tcgcacgccc ataaaatagt cacttgatat tgttcttcga tacgagacag attgtcgagg
      181 atagtttggt gcatgacttt aaaaacaaat tgtatttact aacactgttt cttaatttat
      241 ccttgcgctc cctttaaaaa ggctcttata ataagagttt agaagggtaa aacgtagatt
      301 gattaaggct caaaagacaa taacgaggcg ttatggttct tagctcttat tctcgcatac
      361 atcttgatga atagtgtgta tttttttagg gatacaaaaa tgatgaaaaa attagtcact
      421 tttatggctg ttgtcgcatt aaatggttgt gctgttgtcg cttctcctgt tggtaatggt
      481 gctttgttta cagcaacaaa agggcctgtc acagctacaa atttggactc tgcaagtaaa
      541 tcaggtgaag catgttcaca aaacttgtta ggttttattg tgagtggtga tgcttctatt
      601 gctactgcta aaaaagctgg caatattagt aaagtcgcta cagtcgatca ttcttctgtt
      661 gcatttttag gcttatattc acaattctgt acgcaagtaa caggtgaata atagatcaat
      721 ttagatagat ctaaattgct gatcgcctta tgaaaggtac acataattat catgtgtacc
      781 ttttaatttt aatgtcaaac agcctttgct tgctgtgcgg cattaccaat ataactagca
      841 ggtgttaaag cggctaatgc tgctttggct tcggcaggta aacttgccat tttttcactg
      901 tttaaaaagc tttgaatatc ggcggcggta atgactttgc cacgagtaaa agcttttaat
      961 ttttcgtaag gttcagaaat accaaaacga cgcatcaccg tttgaatagg ttcagccaac
     1021 acttcccaag cattgtctaa atcttctgct aaacgatttg gattaacttc gagtttgcca
     1081 atacctttta aagcagcttc ataagcaatt aagctgtgcg ccaaaccgac acctaagtta
     1141 cgcaatacgg tggagtcagt taagtcacgt tgccaacgag aaataggtaa cttttcacct
     1201 aaatgcgcca atgttgcatt ggcaatacct aagttacctt cggaattttc aaagtcaatc
     1261 gggttaactt tgtgaggcat ggtggacgaa cccacttcac cttcttttaa acgttgttta
     1321 aagtaaccta aagaaatata accccaaacg tcacggttaa agtcaatcaa aatcgtgtta
     1381 aagcgtttta ccgcatcaaa caactcggca atatagtcgt gtggttcaat ttgggtggtg
     1441 taggggttaa agtttaaacc taaaccttca ataaactgtt tggcattctc tgcccaatca
     1501 acatcaggat acgctgacaa gtgtgcattg tagttaccca ctgcaccatt gattttgcct
     1561 aaaatttcaa cggctttaaa ttgttgaatt tggcgaccta aacgataggc cacattcgcc
     1621 atttctttac ccaaggttgt gggtgaggct gtttggccat gagtacgaga caacatgggc
     1681 aaatcggcat aacgatgcgc taattgacga atcgcttcat aaacagccgt catttgttcg
     1741 agagcaatat cacgacctgc ttttaacatc aaaccatgac ttaggttatt aatgtcttcc
     1801 gaagtacaag caaagtgaat aaactcactg actgcggcta attctggatt agcagacgcc
     1861 ttatctttta aaaagtattc aaccgctttt acgtcatggt tagtaatacg ctctttgttt
     1921 ttaatccatt gcgcatcttc ttcgtcaaat aagctgacga tttcttctaa agccatatag
     1981 gtttgtggtg agaattctgg tacttcgcta atttgtgggt gtttgctgag ttgttgtaac
     2041 caacgcactt caacagtaac acgagcgcga attaagccaa actcactaaa ccaaggacgt
     2101 aaactatcgg ctttgctggc atagcggcca tcaacaggag aaagggcagt gagtgtgctt
     2161 agcatgggta cgctccgaca agaaaaaaat caaagcgcga atgataaagg atttactggc
     2221 ctactgcatc ttttgtgtga aatgacgtac tattaacgaa agtttttaca ataaaaagag
     2281 accaataaca tgacaggttt tagtgattac gccaattatg atgctttagg tttggccgat
     2341 ttggtcaaaa agggcgaagt aacaccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnaaca ccccagaatt aggtttgatg ggggtgactg aacctgaatt acatggcgca
     2521 acgcgtaatc cttggaattt acaacgcacg tctggtggtt ctagtggtgg tgcagcggcg
     2581 gcggtggcgg cagggattgt gccaatggct gctgggggtg atggtggcgg ttcattacgc
     2641 attcctgctt cttgttgtgg tttagttggt tttaaaccta gtcgtggtcg caatccttat
     2701 gggcctgcaa tggctgaacc gtggtttggt caagtgcaag aaggcgttat ttcgcgttcg
     2761 gtgcgcgata cagccgcaat gttagatgct acgcatggtc gtgatgttgg ggctttgtat
     2821 gatgcaccta ctgttaaagg cacattttta gcggcggtga gcaaatcgcc taaacgctta
     2881 aaaatcgctt atagccaaga agcattatta agtgatgctt tattagatgc agaatgttta
     2941 cgcggactca aatataccgt tgatttatta agctctttgg gtcatgaagt ggtagaagcc
     3001 acaccaccga ttgataaacg ccaactagcc gatggttatt tattaagaat tgccgcagca
     3061 accgcagggg atgtatttgc cactgaacaa attattggtc gtccagttga actagaagat
     3121 tttgaaaccg aaacatggtc tttagcaaga ttaggacaat cgtttagtgc ctatgattta
     3181 gataatgcta atcgcatgat atatcagcaa gtccgcattt ttgaaacatg gatgcaagac
     3241 tatgatgtgt ttttaacgcc aaccttggca gctccaccga ttgctttagg tggctttcgt
     3301 ttgcaaggag tggagaagtt gttatcacca attgctaaac gttttaaatt aggtggactg
     3361 gtgcattatt tgcctatttt gcagcattta gcacagcaaa attttcaatg ggtcgtctcg
     3421 acacctgtaa tgaatatgac gggtaatcct tcagtgtctt tacctttgca ttggtcacaa
     3481 gataatttgc caataggtat gatgtttacc gcacgttatg gtgacgaaat gactttgttt
     3541 aaattagcag gacaactaga gcaagccaaa ccgtggttta atttgcgtcc tgcaattttt
     3601 gcaggctctt aaccgatgaa aaaatggtgg ttaatcatgt gttcaacatg gtggctaaca
     3661 gcttgccagt ctgtcagtta ttatggccaa agcattaaag gccagtggca aattttggcg
     3721 aatcgtcaac ctttagaaca ggtgattaaa cacccacaaa gctcaccagc cttagtcaaa
     3781 caattgcaat atattcaaca tattagagac ttttctcaag aattatcatt acccactaaa
     3841 ggccaatatg atacctatgt ggatattaaa cgctctgcgg cgatgtggtc ggtggcggca
     3901 acgccagaat tatcattaac ggctaaaaca tggtgtt
//
LOCUS       DAOQMB010000208         3917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11676_length_3917_cov_15.852667, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000208 DAOQMB010000000
VERSION     DAOQMB010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11676_length_3917_cov_15.852667"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1258
                     /gene="mraY"
                     /locus_tag="PLY05_07565"
     CDS             176..1258
                     /gene="mraY"
                     /locus_tag="PLY05_07565"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNC03197.1"
                     /translation="MLLWLADYLAQYYKAFHVFNYLTLRAMLSILTSLAIAIWFGPKM
                     IDYLRKLKFGQAVRTDGPQTHLVKSGTPTMGGALILVSIGVSTLLWANLSNPYIWIVL
                     GVMVVFGAVGWVDDYRKVVEKNPRGLPAKWKYFWQSVGGIAAAVALYYLAKTPAETTL
                     IFPFFKDLVLPLGVFYILLTYLVIVGSSNAVNLTDGLDGLAIMPTVMVAGALALFAYL
                     TGNIKFAEYLHIPYVAGSGELIVICCSIIGAGLGFLWFNAYPAEVFMGDVGALALGAV
                     LGVIAVIVRQEIVLFIMGGVFVMETVSVMLQVGSFKLRGKRIFRMAPIHHHYELKGWP
                     ETKVVIRFWIISFMLVLLGLTTLKIR"
     gene            1266..2621
                     /gene="murD"
                     /locus_tag="PLY05_07570"
     CDS             1266..2621
                     /gene="murD"
                     /locus_tag="PLY05_07570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNC03198.1"
                     /translation="MAGLIQREGLRVVIGLGKTGLSCVRYLRGLGYKVAVNDTRDNPP
                     NLAELQAEFPDVEISLGKLDEHLLLKAHEIIASPGISINEPELLSARLNAGISVIGDI
                     ELFCRAVDKPIVAITGSNAKSTVTTLVGLMAENAGINVAVGGNLGTPVLELLNQPADL
                     YVLELSSFQLETTHSLRAAAAVVLNISEDHMDRYANMQEYHKAKHRIYRNCHFYITNR
                     QDLLTVPLLPEAIPNTSFGLDKPDLNTYGVIREQGKNYLAKGQAKLLATDEMGIFGEH
                     NVANALAALALGEAVNIPMAAMLQTLKAFTGLKHRCQLVVKDQGVAWYNDSKGTNVGA
                     TLAAINGLGAAISGKVLLIAGGVGKGQDFSPLSPALAQYGKALILLGEDAEKIDQAVR
                     ADIPKVHVSSLVAAVQQAQQLAKEGDAILLSPACASFDMFKHYEDRGEQFVAAVKEIT
                     R"
     gene            2672..3865
                     /gene="ftsW"
                     /locus_tag="PLY05_07575"
     CDS             2672..3865
                     /gene="ftsW"
                     /locus_tag="PLY05_07575"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNC03199.1"
                     /translation="MKIALKKPSFSIPRHWIVPDWQQEFTARRLLMLAVAMLACLGVT
                     IVGSASMSIASADFNNPFYFFTRHLVYMMLGVTAATIAANLPMSFWQKWYPVILLIAV
                     ILLIAVLTPLGKRVNGSARWLGVGGLTIQPSEVGKFAVVLYMSIYLVRRQDQVRAGIK
                     GVLPASFILGIVIALFMGEPDFGASLVLMVSVFGMLFLAGAPLKHFMILGVAAVSGAV
                     GLAFSAEYRVKRLMSFWDAWDDAYGTDYQLSQSLIAFGRGDWFGVGLGHSVQKLFYLP
                     EAHTDFVFAIFAEEFGLVGVFVLFFAFYLFVQAGIRIGRNAINKGLLQEGYLAYGMIF
                     LMLMQIIINISVCTGLFPTKGLTLPFISYGGSSLIMVFAMFGILLRIDTETQQLQSTA
                     KGEQK"
BASE COUNT          977 a          667 c          936 g         1337 t
ORIGIN      
        1 tggctattcc tcatggggca accattttgg tgaaaggttc acgcagcgca cgcatggaaa
       61 acgtcgtaca agcattattg gagtaatctc tcaccctgac catctatccc tatgagagag
      121 agttagagtg agggcagaaa attgttaatt tttagttttt aaaggagcaa ccttgatgtt
      181 gctgtggtta gccgattatt tagcacaata ttataaagcc tttcatgtgt ttaattactt
      241 aaccttgcgc gcgatgttaa gtattttaac ctcattggcg attgccattt ggtttggccc
      301 taaaatgatt gattacttgc gtaaattaaa gtttggtcaa gcagtgcgta ccgatggccc
      361 acaaacgcat ttggtcaagt ctggtacgcc aacgatgggt ggtgcattaa ttttagtgtc
      421 tatcggtgtg agtaccttat tgtgggccaa tttaagcaat ccttatattt ggattgtttt
      481 aggggtcatg gtggtttttg gtgcagtcgg ttgggtagac gattatcgta aagtggtcga
      541 aaaaaatccg cgtggcttac ctgctaaatg gaagtatttt tggcagtcag tgggcggtat
      601 cgctgctgct gttgctttgt attatttggc caaaacgcct gccgaaacca ctttaatttt
      661 ccccttcttt aaagatttag tcctgccttt aggcgtattt tatattctgt taacctatct
      721 ggtgattgtt ggtagtagta atgctgttaa cttaaccgat ggtttagatg gtttagcgat
      781 catgcccacc gtcatggtgg caggtgcatt agcccttttt gcttatttaa caggtaatat
      841 taaatttgct gaatatttac atattcccta tgtggctggc tcaggtgaat taattgttat
      901 ttgctgttct attattggtg caggtttagg atttttgtgg tttaacgctt atcccgccga
      961 agtatttatg ggtgatgttg gcgcattagc tttgggtgcg gtgttgggtg tgattgcggt
     1021 gattgttcgt caagaaatcg tcttatttat tatgggcggt gtctttgtca tggaaactgt
     1081 ctcggtcatg ctgcaagtcg gctcgtttaa attacgtggc aaacgtattt ttcgcatggc
     1141 gcccattcat catcactacg aattaaaagg ctggccagaa accaaagtgg tcatccgctt
     1201 ttggattatt agctttatgt tggtgttatt aggtttaact acgttaaaga ttcgttgagg
     1261 catctatggc tggtctgatt cagcgtgaag gtttgcgtgt tgttattggt ttaggcaaaa
     1321 cgggattgtc ttgtgtgcgt tatttgcgtg gtttgggtta caaagttgcg gttaatgata
     1381 cccgcgataa cccaccgaat ttagctgagc tacaagccga gtttcctgat gtggaaatta
     1441 gtttgggtaa gttggatgag catttattat taaaagccca cgaaattatt gccagtccgg
     1501 gtatttcaat taatgagcca gagttattgt ctgcacgttt aaatgctggc atttcggtga
     1561 ttggtgatat tgagttattt tgtcgtgccg ttgataaacc cattgtcgcc attacaggtt
     1621 ctaatgccaa aagtacggtg acgaccttag ttgggctaat ggccgaaaat gcaggcatta
     1681 acgttgctgt tggtggtaat ttgggtacac ctgttttaga actactcaat caacctgcgg
     1741 atttatatgt cttagagcta tctagttttc aattagaaac aacacacagc ttacgcgctg
     1801 cagcggcggt ggtcttgaat attagcgaag atcatatgga tcgttatgcc aatatgcagg
     1861 aatatcacaa agccaaacac cgcatttatc gtaattgcca tttttatatc accaatcgcc
     1921 aagatttatt aactgtgcct ttgttgccag aagcaattcc caataccagt tttggtttag
     1981 ataagcctga tttaaatacc tatggcgtga ttagggagca aggcaaaaat tatttggcca
     2041 aaggacaagc aaaactgtta gcaacagacg aaatgggtat ttttggtgag cataatgtcg
     2101 ctaatgcttt agcggcttta gcattaggtg aagccgttaa tattccaatg gcggcgatgt
     2161 tgcaaacctt aaaagcattt acagggctaa aacatcgttg ccagttagtc gttaaagatc
     2221 aaggagttgc ttggtataac gattctaaag gcactaacgt tggcgcaact ttagcagcaa
     2281 ttaatggttt gggtgcagca attagcggca aagtattatt gattgcaggg ggagtgggta
     2341 aaggccaaga ttttagtcct ttgtcacccg ccttagcaca atatggcaaa gccttaattt
     2401 tattaggcga agatgctgaa aaaattgatc aggccgttcg tgcggatatt cctaaagttc
     2461 atgtctctag tttagtggct gctgtacaac aagctcaaca attagccaaa gaaggggatg
     2521 ctattttgtt atctccagcc tgtgcgagct tcgatatgtt taaacattat gaagatcgtg
     2581 gtgagcaatt tgttgccgca gttaaagaga tcacccgtta attattccct cttaaccctc
     2641 tttcaaagag ggtatttact gagcatcatt tatgaaaatt gcgctcaaaa aaccttcttt
     2701 ttctattcct agacattgga ttgtccctga ttggcaacaa gaatttactg ctcgtcgttt
     2761 attgatgtta gcagtggcga tgttagcttg tttaggggtg acgattgttg gctcggcatc
     2821 gatgagtatt gccagtgctg actttaataa tcctttttat ttttttacac gccacttagt
     2881 ttacatgatg ttaggtgtca cagcggcaac gattgccgct aatttgccca tgagtttttg
     2941 gcaaaaatgg tatcccgtca tcctactgat tgcggtaatt ttgctgattg cagtgttaac
     3001 gccattaggt aaacgtgtta atggtagtgc cagatggttg ggggtgggtg gtttaaccat
     3061 tcagccatca gaagtgggta aatttgctgt cgttttatat atgtcaattt atttagttcg
     3121 ccgccaagac caagtacgag caggcattaa aggtgtttta cctgcaagtt ttatcttagg
     3181 tattgtcatt gccttattta tgggtgaacc agactttggt gctagtttag tattaatggt
     3241 ttcggtattt ggtatgttgt ttttagcagg tgcgccactt aaacacttta tgattttagg
     3301 ggttgctgcg gtcagtggtg cagtgggttt ggctttttct gccgaatatc gagttaaacg
     3361 tttaatgtca ttttgggacg catgggacga tgcctatggc acggactatc aattatcaca
     3421 aagtttgatt gcctttggtc gtggtgattg gtttggtgta ggtttaggcc atagtgtgca
     3481 aaaactattc tatttgcctg aagcacatac tgacttcgta tttgctattt ttgctgaaga
     3541 atttggtttg gttggtgtat ttgttttgtt ttttgccttt tatttatttg tgcaagctgg
     3601 tattcgtatt gggcgaaatg ccattaataa gggcttgtta caagaaggct atttagctta
     3661 tggcatgatt tttttaatgt taatgcaaat tattattaac atttcagttt gcacaggctt
     3721 atttccaacc aaaggcttaa ccttaccctt tatcagttat ggtggcagta gcttaattat
     3781 ggtatttgcg atgtttggta ttttgttgcg aatagatact gaaactcagc aattacaatc
     3841 gaccgcaaaa ggagagcaaa aatgaccgtt aaatcggtag tggtgatggc aggtggtaca
     3901 ggtgggcatg tttttcc
//
LOCUS       DAOQMB010000209         3910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11706_length_3910_cov_12.029313, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000209 DAOQMB010000000
VERSION     DAOQMB010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11706_length_3910_cov_12.029313"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1324)
                     /locus_tag="PLY05_07580"
     CDS             complement(44..1324)
                     /locus_tag="PLY05_07580"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNC03200.1"
                     /translation="MILAKLTQRFTRRPLATALGLSLLVHLSLLWDGEFELPDLLADN
                     TDEVLSKKKAESVPRVKLNIKPPARPKAQLGATIVTIVEEAPPAANKVAPKEQPKAPK
                     KQLAKNPTKPKVEEPPILSGDPLPNSHSNNTVPENPQTDNQTQGTPDNAVPLGDTVPP
                     PPEELAKTPPTPPVNEPPPSFPIEIMATYKASFMGFSGNIKQVWRMEGESYYIENEAS
                     KFGFSVKMVSEGSVSQSGIHPEHYKMYVNNKLRNFADIDRSTSSIRYGKNNDVKYSAL
                     QGEIQDAASLPFQVAVNFEAGKNNQMQVTTGSAIYNIDLKIIAQEDIKLPAGVLRTLH
                     LQGQRINISTGTALQGYDVWLAIDYRNFPVKFRGPTSKGEVLEYSVKSLSFEGQTVLG
                     KNIKPEFEANEPTTLPPELLEQKQPTISVGDDAP"
     gene            complement(1339..2622)
                     /gene="hemL"
                     /locus_tag="PLY05_07585"
     CDS             complement(1339..2622)
                     /gene="hemL"
                     /locus_tag="PLY05_07585"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004936403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNC03201.1"
                     /translation="MSRNQELFAAACRHIPGGVNSPVRAFKGVGGTPIFFKKGQGAYL
                     WDADDKPYIDYVGSWGPMILGHAHPDIVKAVQEAAVDGLSFGAPTEAEINMADRVCEL
                     MPSMDMVRMVSSGTEATMSAIRLARGYTKRDKLLKFEGCYHGHADSLLVKAGSGLLTL
                     GVPTSLGVPADLAQHTLTLPYNNIDALQQLFAQLGHEIACVIVEPVAGNMNCVPPSRE
                     FLQTMRDLCSQYGAVLIFDEVMTGFRVALGGAQALYNIKPDLTCLGKVIGAGLPVGAF
                     GGKREIMECIAPLGGVYQAGTLSGNPLAMRAGLAMLALISQTGFYEQLAANLSKLLAG
                     LQQRADAANIPFTTTQVGGMFGLFFTSKKDISSYDDVMACDVERFRRFFHLMLEEGVY
                     LAPSAFEAGFISSKHGDAEIQATLDAAERVFARLS"
     gene            complement(2638..3246)
                     /gene="thiE"
                     /locus_tag="PLY05_07590"
     CDS             complement(2638..3246)
                     /gene="thiE"
                     /locus_tag="PLY05_07590"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNC03202.1"
                     /translation="MTQLYLITDSQLLAGRLLPAVEQALIGGAKLIQYRDKSTDTSKR
                     FHEAQQLKALCQCYNVPLIINDDLELAAQLGVGVHLGQQDGSIALARQRLGQDAIIGA
                     TCHNSLEFAKQAATQGASYLAFGAFYPSSTKPLAQAADLQTLTQAKQLFSLPVVAIGG
                     INLDNARPLIDAGADYLAVISDIFALPTANICQQAEAYLRLF"
     gene            complement(3357..>3910)
                     /locus_tag="PLY05_07595"
     CDS             complement(3357..>3910)
                     /locus_tag="PLY05_07595"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HNC03203.1"
                     /translation="AIKCGMLGTPETVEVVKQICQKNPHIPLILDPVLVANSGGSLTV
                     EGFIDSLKQLFPYAHLLTPNTVEAERLTGLSVADNELDVISTLTDLGVKAILLKGGHE
                     QGQMLRNRLYQDNKLIQTSHWPRLNAQFHGSGCSLASAISAGIANGLPLPIAVTRAEV
                     WLQRSLQNADFPHKNGQAIPRRI"
BASE COUNT         1133 a          825 c          827 g         1125 t
ORIGIN      
        1 aatctaatta tttggcatat ctatatagct ttaacatata acattaaggt gcatcatcac
       61 caacactaat cgttggttgc ttttgttcta ataattcagg tggtaaagta gttggttcat
      121 tagcctcaaa ctcaggctta atatttttac ccaatacggt ttggccttca aaacttaatg
      181 atttaacact gtattctaat acttcgcctt tgcttgtcgg gcctctaaat ttaacaggaa
      241 agtttcgata atcaatggct aaccaaacat catagccttg taaggcagta cctgtgctaa
      301 tgttaatgcg ttgaccttgt aagtgtaggg ttcttaaaac cccagcaggc agtttaatat
      361 cttcttgggc aataattttt aaatcaatat tataaatggc actacctgtg gttacttgca
      421 tttggttatt tttgccagct tcaaaattga cggcaacttg aaatggcaag ctggctgcat
      481 cttgaatttc gccttgtaac gcagaatatt tcacatcgtt atttttgcca tatcgaatac
      541 tgcttgtact tcggtctata tcagcaaaat tacgcaattt gttattaaca tacattttgt
      601 agtgttcggg atgaatacca ctttgtgaca cactgccttc actcaccatt ttgacactaa
      661 agccaaattt agacgcttca ttttcaatat agtaactttc accttccata cgccaaactt
      721 gtttaatgtt gccactaaag cccataaatg aggctttgta ggttgccata atttcaatgg
      781 ggaaagaggg gggcggttca ttaacaggtg gtgtaggtgg tgttttggct aattcttcgg
      841 gtggcggagg tacagtatcg cctaaaggca cagcattatc aggcgtgcct tgggtttgat
      901 tgtcagtttg tggattttct ggaacagtat tattgctatg actgttgggt aaagggtcgc
      961 cacttaaaat cgggggttct tcgactttag gtttagtggg atttttggcc aattgttttt
     1021 taggggcttt gggctgctct ttgggagcaa ctttattggc cgcaggtggt gcttcttcga
     1081 caatagtgac aatcgttgcg cctaattgtg ctttaggacg tgcagggggc ttaatattga
     1141 gtttaacgcg tggtacagac tccgcttttt tcttagacaa cacttcatcg gtgttatcag
     1201 caagtaagtc aggtagctca aattcaccat cccataacaa ggacaaatgc actaacaacg
     1261 ataaccccaa agcagtcgct aaggggcggc gtgtaaatcg ttgtgttagt ttggctaaaa
     1321 tcacgcgtta acaacctatt agcttaaacg tgcaaacaca cgttctgctg catctaatgt
     1381 tgcttgtatt tctgcgtccc catgtttgct agaaataaag ccagcttcaa aagcagaagg
     1441 ggctaaatac acgccttctt ctaacattaa atggaagaaa cggcggaaac gctcaacatc
     1501 acaagccatg acatcgtcat aagaactaat gtcttttttg ctggtaaaga ataaaccaaa
     1561 cataccgcct acttgggtgg tggtaaaggg aatattcgca gcatcagcac gttgttgtaa
     1621 gccagctaag agtttgctca aattggcggc aagctgctca taaaaaccag tttggctaat
     1681 taaggctaac attgctaagc ctgcacgcat tgctaaagga ttgcctgata atgtccctgc
     1741 ttgatacaca ccgcctaaag gtgcgataca ttccataatt tcacgtttgc caccaaaagc
     1801 gcccacaggt aaaccagcac caatcacttt acctaagcaa gttaaatcgg gtttgatgtt
     1861 gtataaggct tgtgcgccac ctaaggcaac cctaaaacct gtcatcactt catcaaaaat
     1921 taataccgca ccatattggc tacataagtc acgcatggtt tgtaaaaatt cgcggcttgg
     1981 tggtacacag ttcatgttac ctgcaacagg ctcaacaatc acacaagcaa tttcgtggcc
     2041 aagctgggca aacagttgct gtaaagcgtc gatattattg taggggagag tcagtgtgtg
     2101 ttgggctaaa tcggcgggta cacctaaaga ggtgggtaca cctaaggtta ataagcctga
     2161 gccagctttc actaataaag agtccgcatg gccgtgataa caaccttcaa attttaataa
     2221 tttatcgcgt ttggtgtagc cacgagccaa acgaattgca ctcatggtgg cttctgtgcc
     2281 agagctaacc atacgcacca tatccataga gggcattaat tcacacacac gatcagccat
     2341 attaatttcg gcttcggtcg gcgcaccaaa acttaaacca tcaaccgcgg cttcttgtac
     2401 ggctttaaca atatcaggat gggcatgacc taaaatcatc ggcccccatg agccaacata
     2461 atcaatataa ggtttgtcat cggcatccca caaataagcg ccttggcctt ttttgaagaa
     2521 aattggtgta ccacctacgc ctttaaaagc acgtacaggc gagttgactc caccagggat
     2581 atgacgacaa gcagcagcaa ataattcttg attacgactc atggagaaac tctataacta
     2641 aaataaccgt aaatacgcct cagcctgttg gcaaatattg gctgtaggca aagcaaatat
     2701 atcactaatt accgcaagat aatcagcccc tgcatcaatc agtggtcgag cattgtccaa
     2761 gtttatgccg ccaatagcaa ctacaggtaa gctaaacaat tgtttggctt gcgttaatgt
     2821 ctgtagatcg gctgcttgtg ctaagggttt ggtgctggat ggataaaatg cgccaaaagc
     2881 taaatagctt gcgccttgag tggcggcttg tttggcaaac tcaagcgagt tatggcaggt
     2941 tgcgccaata attgcgtctt gacccaaacg ttgacgcgct aaagcaattg agccatcttg
     3001 ctgaccaaga tgcacaccaa cccctagttg cgctgcaagc tcaagatcat cattaataat
     3061 caagggtacg ttgtagcatt ggcatagggc tttaagttgt tgtgcttcat gaaaacgttt
     3121 gctggtgtcg gttgatttgt cgcgatattg aattaacttt gcaccaccaa ttaaggcttg
     3181 ctcaacagca ggcaataaac gtcctgctaa taactggcta tcggtgatga ggtagagttg
     3241 cgtcattaaa tttgcccaaa aagataccgt aatgtcagtg agttaaatga ttattgttgg
     3301 tttcgactcc gctcaaccaa cgaaaaatgc accctgagcg gagtcgaagg gatattttaa
     3361 atacgtcttg gaatggcctg tccattttta tgaggaaaat cagcgttttg taaactgcgt
     3421 tgtaaccaaa cttcagcacg agtaaccgca attggcaacg gtaaaccatt agcaatacca
     3481 gcactaatcg ctgatgctaa actacaacca gaaccatgaa attgagcatt taaacgcggc
     3541 caatgactgg tttgtataag tttattatct tgatataaac gattacggag catttgaccc
     3601 tgttcgtgac cgcctttaag caagatggct tttacaccta aatcagttaa agtactaatg
     3661 acatctaact cattatcagc aacagataat cctgttaaac gctcagcttc tacggtattg
     3721 ggtgttaaca aatgagcata gggaaacaat tgtttaaggc tgtcaataaa cccctcaaca
     3781 gtgagtgagc caccactatt agcgactaag acagggtcta aaatgagtgg aatatgagga
     3841 tttttttggc aaatttgttt aaccacttca actgtttcgg gtgttcccaa catgccacat
     3901 ttaatggctt
//
LOCUS       DAOQMB010000210         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11719_length_3907_cov_13.721963, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000210 DAOQMB010000000
VERSION     DAOQMB010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11719_length_3907_cov_13.721963"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="PLY05_07600"
     CDS             complement(<1..971)
                     /locus_tag="PLY05_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009508435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNC03204.1"
                     /translation="MSSSSLSISGSAVKPLFAYDKYWAECFGAAPFLPMSRAEMDSLG
                     WDACDIILVTGDAYVDHPSFGMAIIGRLLEAQGFRVGIIAQPDWNSAQDFKKLGEPTL
                     FFGVTAGNMDSMINRYTADRKIRSDDAYSPHGEGGKRPDRCSLVYAQRTREAYPHIPI
                     VMGGIEASLRRIAHYDYWQDKVRRSLLLDAKADLLVFGNAERAIVDIAHRVANGDNIR
                     SITDIRGTAFMRRDTPEGWFELDSTNVDEIGRVDPIINPYVNTQDLAVCDVEKEKGLT
                     ETDVQVVTLQPSKALKHRLPARDKTVIRLPAYEQVKNDPVMYAHANRV"
     gene            1198..1998
                     /gene="rsmA"
                     /locus_tag="PLY05_07605"
     CDS             1198..1998
                     /gene="rsmA"
                     /locus_tag="PLY05_07605"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNC03205.1"
                     /translation="MTDSLHGHKARKRFGQNFLEDDNVINNIVHAIAPQVGDNMVEIG
                     PGLAALTRFLLPACQKLHVIELDRDLAARLPNTLAGLGELHLHQADALNFDFAQLASI
                     EHPLRIVGNLPYNISTPLIFHLLSFSHIVKDMTFMLQKEVVDRLAGEPSTADYGRLSV
                     MVQYRCQVDWLLDVPPEAFNPPPKVQSAVVRLTPWQNSPYNCQNETLLQHIVSTAFNQ
                     RRKTLRNCLKGLAEDADFIAVDIDPSLRAEVLSVQQFVQLTNQLNRHV"
     gene            1991..2371
                     /gene="apaG"
                     /locus_tag="PLY05_07610"
     CDS             1991..2371
                     /gene="apaG"
                     /locus_tag="PLY05_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HNC03206.1"
                     /translation="MSNTEHQIIVSVKSEYLAEQSSPQDSRFVFAYHISIQNQGAKKA
                     QLLTRHWIITDGEARTEEVRGDGVVGEQPQIEAGSTYKYSSGAILKTPVGSMHGSYGM
                     IDEQGQHFEAAIPAFSLAVPRMLH"
     gene            2497..>2622
                     /locus_tag="PLY05_07615"
     CDS             2497..>2622
                     /locus_tag="PLY05_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC03207.1"
                     /translation="MADYAIGDVQGCADALEELLEVINFDPQHDCLWFAGDLVARG"
     assembly_gap    2623..2722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2723..3076
                     /locus_tag="PLY05_07620"
     CDS             <2723..3076
                     /locus_tag="PLY05_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HNC03208.1"
                     /translation="PALWHEHLRGAKRWRVITNYLTRMRLCNLAGELEFSHKEGLDDL
                     PDGYYPWFNLPNPSIKNTRILFGHWAALEGNGPQAPIIALDGGCVWGGQLLAYRLDDG
                     RFFSATKACIDCGLE"
     gene            3241..>3907
                     /locus_tag="PLY05_07625"
     CDS             3241..>3907
                     /locus_tag="PLY05_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03209.1"
                     /translation="MSLFQHYQSRFDHSRPEEYTLEQYLNICKNDATAYATAAERLLM
                     AIGEPEVIDTSRDPRLSRIFSNKVIRRYPAFSEFYGMEEAVEKIVSYFRHAAQGLEEK
                     KQVLYLLGPVGGGKSSLAERLKSLIEKTPFYAIKDSPINESPLGLFNIEEDGDILEQD
                     YNIPKRYLRGIMSPWAVKRLQEFGGDISKFKVVKIYPSILHQLAVSKTEPGDENNQDI
                     SALV"
BASE COUNT         1122 a          794 c          760 g         1131 t
ORIGIN      
        1 acacgattag catgagcata catgacaggg tcatttttga cttgttcata agcaggcaaa
       61 cgaatcacgg ttttatcacg tgctggcaaa cggtgcttta atgctttact gggttgcagg
      121 gtaacaactt gcacatcagt ttcggttaag cctttttctt tttcaacgtc acaaacggct
      181 aaatcttgag tattaacata gggattgata atagggtcaa ctctccctat ctcatcaaca
      241 ttggttgaat ctaattcaaa ccaaccttct ggcgtatcac gacgcataaa cgctgtgcca
      301 cgaatatccg taatactgcg aatgttatca ccattggcta cgcgatgagc aatatcaaca
      361 atggcacgtt cagcattacc aaacaccaat aaatcggctt tcgcatctag caataacgaa
      421 cgacggactt tatcttgcca ataatcataa tgtgcaatac gccgtaaact ggcttcgata
      481 ccacccatca caatcggaat atggggatag gcttcgcgtg tacgttgggc atacactaag
      541 gagcagcggt caggacgttt accaccctcg ccatgtggtg aataggcatc gtcggagcgg
      601 attttacgat cagccgtata acggttaatc atcgaatcca tattgcctgc ggtgacgcca
      661 aaaaacagcg ttggctcacc gagctttttg aaatcttggg cagaattcca atcgggctgc
      721 gcaataatac ctaccctaaa tccttgcgct tctaataaac gaccaataat ggccatgcca
      781 aaactgggat ggtcaacata ggcatcacct gtcaccaaaa taatgtcgca agcatcccag
      841 cccaaactat ccatttcagc acgactcatc ggcaaaaatg gggcagctcc aaaacactct
      901 gcccagtatt tatcgtaggc gaataaaggc ttaacagcag aacctgaaat agataggctg
      961 gacgatgaca tagcaattgc actcaagtaa atagcttttc tattataccc gactaaacta
     1021 ctcagaaaaa cattgttttc aataacaacc ttgagaggct gttgatcttt tattttttct
     1081 agatcaacac ttcaaacaaa acaatcagtg gatgatgaaa acacactaaa ttcgatgaaa
     1141 tgcgaaagtc ttgctattca tgtataattt cacaaatttt ttgatgactg ataaataatg
     1201 accgactctt tacacggcca caaagctcgc aaacgttttg gccaaaactt tttagaagat
     1261 gataacgtca tcaataatat tgttcatgcc attgccccac aagtgggtga caatatggtt
     1321 gaaattgggc ctggtttagc ggcattaact cgctttttat tacctgcttg tcaaaaactt
     1381 catgttattg agcttgatcg tgatttagcc gctcgtttgc caaatacctt agcaggttta
     1441 ggcgaactac atttgcacca agctgatgcc ttaaactttg attttgcaca attagcaagc
     1501 atcgaacatc ctttaagaat cgttggcaat ctcccttaca atatttctac accattaatc
     1561 tttcatttat tgagctttag ccatatcgtc aaagacatga cgtttatgct acaaaaggaa
     1621 gtcgttgacc gtttagcagg agaaccgtca accgctgatt atggtcgttt gagtgtcatg
     1681 gtacagtatc gttgccaagt ggattggtta ttagatgtac cacccgaagc atttaatcca
     1741 ccacctaaag tacaatctgc ggttgtacgc ttaacacctt ggcaaaattc accgtataat
     1801 tgccaaaatg aaactttact gcaacatatt gtcagcacag cctttaatca acgtcgcaaa
     1861 accttgcgta actgtttaaa aggattagcg gaagatgctg attttattgc agtcgatatt
     1921 gaccccagct tgcgtgctga ggttttaagc gttcaacaat ttgtgcaact tactaatcag
     1981 ttaaaccgtc atgtctaata ccgaacacca aattattgtc agcgttaaaa gtgagtattt
     2041 agccgagcaa tctagcccac aagacagtcg ttttgtgttt gcttaccata ttagtattca
     2101 aaatcaaggt gccaaaaagg ctcaattatt aacccgccat tggattatta ccgatggtga
     2161 agcccgtacc gaagaagttc gtggtgacgg tgttgtcggt gagcaaccac aaatcgaggc
     2221 gggttcgact tataaatata gcagcggtgc tattttaaaa acgcctgtcg gtagtatgca
     2281 cggttcttat ggcatgattg atgagcaagg ccagcatttc gaagcagcaa tccctgcttt
     2341 tagtcttgct gtgcctagaa tgctacatta ataggactta cgcggttatc gcttatttgc
     2401 tcaccaaata atcgttttca acgatttttt cgttcacaaa acgcgttaat ttcggtgaaa
     2461 tgcgtaagtc ctaactaatt aatagagaga aaatacatgg ctgactatgc catcggtgat
     2521 gtacaaggct gtgctgatgc ccttgaagaa ttactagagg tcattaattt tgaccctcag
     2581 cacgattgct tgtggtttgc gggcgacctt gtcgctcgtg gcnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nncctgcttt atggcatgag catcttcgag gtgcaaaacg
     2761 ttggcgtgtc atcaccaatt atttaacccg tatgcgtctg tgtaatttag caggtgagtt
     2821 agagtttagt cataaagaag gtttagacga tttgccagac ggttattatc cttggtttaa
     2881 tttgcctaat cctagcatca aaaacactcg tattttattt ggtcactggg cagcactcga
     2941 aggaaatggc cctcaagcac cgatcattgc tttagatggt ggttgtgttt ggggtggaca
     3001 attattagcc taccgcttag atgatggtcg cttttttagt gccactaaag cgtgtattga
     3061 ttgtggatta gaatagactc tctgatacct taaagcttgg cacaagccct gcatgatttt
     3121 atttagttct taatttttaa cataaacgct aaaaactttt ttaggcaaaa ttgtctttta
     3181 gtgtctaagc tgaatatata aggacactgt tatttacagt ataaaatcaa gaggttcgtt
     3241 atgagtttgt ttcaacatta tcaatcacgt tttgatcata gccgtcccga agaatatact
     3301 ttagagcaat atcttaatat ttgtaaaaat gatgctacgg cttatgctac cgctgccgaa
     3361 cgtttattaa tggcgattgg cgagccagaa gttattgata ccagtcgtga tccacgtctt
     3421 tcaagaattt tttctaacaa agtcattcgt cgctatcctg catttagtga attttatggc
     3481 atggaagaag ccgttgaaaa aattgtttct tatttccgcc acgccgcaca aggcttagaa
     3541 gagaaaaaac aagttttata tctattaggc cctgtcggtg gcggtaaaag ctctttggca
     3601 gaacgactca aatctctcat agaaaaaaca cctttttacg ctattaaaga ctcaccaatt
     3661 aacgaatcac cgttaggttt atttaatatc gaagaagatg gcgatattct tgagcaagat
     3721 tacaacatcc ccaaacgtta tttacgtggc atcatgtcgc cgtgggcagt taaacgttta
     3781 caagagtttg gcggtgatat tagtaaattt aaagtggtta aaatttaccc cagtatttta
     3841 catcaactcg ctgtttctaa aactgaacct ggtgatgaga ataatcaaga catttccgca
     3901 cttgtgg
//
LOCUS       DAOQMB010000211         3898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11778_length_3898_cov_15.102524, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000211 DAOQMB010000000
VERSION     DAOQMB010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11778_length_3898_cov_15.102524"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..1148)
                     /gene="serC"
                     /locus_tag="PLY05_07630"
     CDS             complement(66..1148)
                     /gene="serC"
                     /locus_tag="PLY05_07630"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNC03210.1"
                     /translation="MRAYNFCAGPAALPEAVLKQAQAELVDYQGKGLSVMEMSHRSAD
                     YVAIAEQAEQDLRDLLNVPSNYKVLFLQGGASQQFAMIPMNLLRGKTSADYVNTGMWS
                     DKAIKEAKKFCQVNVVASDEANNFRAVPAFESWQRNADAAYLHYTPNETIHGVQFDFI
                     PEVGDVPLVADYSSIILAEPLDVSKFGLIYAGAQKNIGPAGLVVVIVREDLLGQTIKG
                     TPTMLDYKIHADNGSMYNTPSTYAWYLSGLVFKWLKTQDGLEKMAEINRRKATKLYAA
                     IDGSGGFYANPVAIANRSMMNVPFTLADSSLDKAFLKEAEANQLLNLAGHRSVGGMRA
                     SIYNAVPEEGIDTLVSFMQDFAKRHG"
     gene            complement(1203..>1635)
                     /locus_tag="PLY05_07635"
     CDS             complement(1203..>1635)
                     /locus_tag="PLY05_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005169983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HNC03211.1"
                     /translation="DSDEVVLKAGQAQVLIACANGYGKRTPVSKFTLRKRGGQGVVAI
                     QTSERNGELVAAVQAGEGKDLLLISDMGTLVRTRIDEVSVSSRTAQGVTLIRLSNDEQ
                     LVAVVSLDAPEEEVIEADNTEEVVVATETVVTSTNEQAEAV"
     assembly_gap    1636..1735
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1736..3870)
                     /gene="gyrA"
                     /locus_tag="PLY05_07640"
     CDS             complement(<1736..3870)
                     /gene="gyrA"
                     /locus_tag="PLY05_07640"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNC03212.1"
                     /translation="MTEFAPEIVPVNIEDELRQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHELGNDWNKSYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYLL
                     VDGQGNFGSVDGDAPAAMRYTEVRMTRLAHDLLADLEKETVDWLPNYDGSEMIPAVLP
                     TRIPNLLVNGSSGIAVGMATNIPPHNITEVINACLAYVDDNDITVDGLMQYVTGPDFP
                     TGGIINGRSGIVDAYRTGRGRILIRAKYHIETDSKTSRHTVVFTEIPYQLNKARVIEK
                     IAELVKDKKIEGISELRDESDKDGMRIVIDLKRGENPEVLVNNLFAQTPLESSFSFNM
                     LALQDGQPRILNLRDLIAAFVRHRQEVVTRRTVFELRKARERGHIVEGLAVALANIDE
                     IIQLIKTSATGAQAKERLLEKVWKEGGVLALLERAGDKNACRPETLPEYLGLVENGYK
                     LSPEQAQAILDLRLHRLTGLEQDKLLAEYEELLGKIAALLAILADSNLLMDVIRGELK
                     EVLATYGDGRKTEIIASRLDLSREDLTPDEQVVLTISHTGYAKTQPLSDYKAQKRGGR
                     GKSATSMKDEDYIEHLLVASTHDTVLCFTSLGRVHWIKVYDLPQAGRGSKGKPLVNLL
                     QLANDEKVTAILPLREYAEGQYIFMATADGTVKRVELSAFSRPRSLGLNAINLEGDNT
                     LIGVAVTDGQQDIMLFSNQGKAIRFRETDV"
BASE COUNT         1111 a          890 c          712 g         1085 t
ORIGIN      
        1 ttctttgaat aataaaaaaa ggcttagatt ttactctaag ccttttttct tcgccgtcag
       61 cttaattaac catgacgttt agcaaaatct tgcataaaac tcactaaggt atcaatgcct
      121 tcttcgggaa cggcattata tatacttgca cgcataccac cgacagagcg atgacctgct
      181 aagtttaata attgattagc ttcggcttct tttaagaaag ctttgtctaa actgctatct
      241 gctaaggtaa atggcacatt catcattgag cggttggcaa ttgctacagg gtttgcataa
      301 aaaccaccac taccatcaat tgctgcatac aattttgtgg ctttacgacg gttaatttcg
      361 gccatttttt ctaggccatc ctgagttttt aaccacttaa acactaaacc agacaaatac
      421 cacgcataag ttgatggtgt gttatacata gagccattat cagcatggat tttgtagtca
      481 agcatggttg gcgtgccttt gatggtttga cccaataagt cttcacgcac aatcacgacc
      541 actaaacctg ctgggccaat atttttttgt gcacccgcat aaattaagcc aaatttagac
      601 acgtctaaag gttcagccaa aataatcgac gagtaatcgg ccactaacgg cacatcaccc
      661 acctcaggaa taaagtcaaa ttgcacacca tgaatcgttt catttggggt atagtgcaaa
      721 taagcggcat cagcattacg ttgccatgac tcaaaggctg gcactgcgcg gaagttattg
      781 gcttcgtcac tggcaactac atttacttgg cagaattttt tggcttcttt aatggcttta
      841 tcagaccaca taccggtatt aacataatcg gcagaagttt taccacgcaa caaattcatc
      901 ggaatcatgg caaattgttg tgatgcaccg ccttgtaaaa ataatacttt gtagttactg
      961 ggcacattta ataaatcacg taagtcttgc tctgcttgtt cagcaatcgc aacataatcc
     1021 gcgctacggt gactcatttc catcaccgac aaacctttgc cttgatagtc aaccaattcg
     1081 gcttgggctt gttttaagac ggcttcgggt aatgcagctg gacctgcaca aaagttatat
     1141 gctctcatga aaatacctta actcattgag cctctaacag aggctctgtc aaacttaaat
     1201 cattaaaccg cttcagcttg ttcgttagtg ctagttacga ctgtttctgt tgcaacgacc
     1261 acttcttcgg tgttatccgc ctcaatcacc tcttcttcgg gtgcatcaag actgactacc
     1321 gccaccaact gctcgtcatt acttaatcta atgagcgtaa caccttgtgc tgtacgagat
     1381 gacaccgaca cttcatcaat acgagtacgc actaaagtac ccatatcaga aattaataat
     1441 aagtctttgc cttcacctgc ttgcacagcg gctactaact caccattacg ctcactggtt
     1501 tgaatggcca caacgccttg tccaccacgt ttacgcaagg taaatttgct cactggtgta
     1561 cgtttgccat aaccattagc acaagcaatt aatacttgtg cttgaccagc ttttaacact
     1621 acttcatcgc tgtcannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacatc
     1741 ggtttctcta aagcgaatcg ctttaccctg attgctaaac aacataatgt cttgttggcc
     1801 atcagtcact gcaacaccaa ttaaggtgtt atcaccttct aaattaatgg catttaaacc
     1861 taagctacgt ggacgagaga atgcagataa ttccacgcgt tttaccgtac catctgcggt
     1921 tgccatgaag atatattgac cttcggcata ttcacgcaat ggtaaaatag cggtaacttt
     1981 ttcgtcattg gctaattgca acaagttaac taatggcttg ccttttgaac cgcggccggc
     2041 ttgtggcaag tcatacactt taatccaatg cacacgacct aaagaggtaa aacacaatac
     2101 agtgtcgtga gtgctcgcca ccaataaatg ttcaatataa tcttcatctt tcatgctggt
     2161 tgcagattta ccacgaccac cacgtttttg cgctttataa tcacttaaag gctgtgtttt
     2221 ggcataacct gtatgagaaa tggttaaaac tacttgctca tctggtgtta aatcttcacg
     2281 gcttaaatct aaacgagagg caataatttc ggttttacga ccatcgccat aagtggctaa
     2341 aacttctttt aactcgccac gaatcacatc catcaacaaa ttagaatcgg ccaaaatagc
     2401 taatagtgca gcaattttgc ctaataactc ttcgtattct gctaagagtt tatcttgttc
     2461 taaacctgtt aaacggtgta aacgtaaatc taaaatcgct tgggcttgtt cgggcgataa
     2521 tttgtagcca ttttcaacca aacctaaata ttcaggtaag gtttcaggac gacaggcatt
     2581 tttgtcacct gcgcgctcta acaacgctaa aacaccacct tctttccaca ctttttctaa
     2641 taaacgctct ttggcttgtg cgcctgttgc tgaggtttta attaattgaa taatttcatc
     2701 aatattggct aaagcaaccg ctaaaccttc aacaatatga ccgcgttcac gcgctttgcg
     2761 taattcaaaa acggtacgac gggtaacaac ttcttgacga tgacgcacaa aagcagcaat
     2821 caagtcacgc aaattgagaa tgcgtggctg accgtcttgt agagccaaca tattaaagct
     2881 aaaagaagac tctaacggtg tttgagcaaa caagttattg actaatactt cggggttttc
     2941 accacgtttt aagtcaataa caatacgcat accatctttg tcagactcgt cacgtaattc
     3001 gctaatgcct tcaatctttt tgtctttgac taattcggca attttttcaa tgacacgggc
     3061 tttgttaagc tgataaggaa tttcggtaaa cactaccgta tgacgagagg ttttgctgtc
     3121 ggtttcaata tggtatttcg cacgaattaa aatgcggcca cgacccgtac ggtaagcatc
     3181 aacaatacca ctgcgaccat taataatgcc acctgttgga aagtctgggc cagtcacata
     3241 ctgcatcaaa ccatcaacgg taatgtcatt atcatcaaca taagccaaac aggcattaat
     3301 cacttcagta atgttgtgtg gtggaatatt ggtggccata cccacggcaa tacccgatga
     3361 accattgact aataaattgg gaatacgcgt tggtaacacc gcaggaatca tttcgctgcc
     3421 atcatagttg ggcaaccaat cgacggtttc tttttcgagg tcagccaaca aatcatgcgc
     3481 caaacgggtc atacgcactt cggtataacg catcgcagca ggcgcatcgc cgtccaccga
     3541 accgaagtta ccttgaccat caaccaataa ataacgcaag ctaaatggct gtgccatacg
     3601 cacaatggta tcatacaccg ccgtatcacc atgagggtga tatttaccga tgacatcacc
     3661 gacaacacga gcggattttt tataggattt attccagtcg ttaccaagtt cgtgcatcgc
     3721 aaataacaca cgacgatgaa caggctttaa gccatcacgc acatcgggca aagcgcggcc
     3781 aacaataacg ctcatggcat aatctaaata ggattggcgt aattcgtctt caatattgac
     3841 agggacgatt tctggggcaa attctgtcat gcgtgttctc gacgggcata aaaaggga
//
LOCUS       DAOQMB010000212         3897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11785_length_3897_cov_16.093441, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000212 DAOQMB010000000
VERSION     DAOQMB010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11785_length_3897_cov_16.093441"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLY05_07645"
     CDS             complement(<1..441)
                     /locus_tag="PLY05_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC03213.1"
                     /translation="MKDDVIQRIGPNKVVTLHFAVCLANGQLLDTTREREQPVSFTVG
                     DGSLLEGFEKAIFGLKAGDKRSIFIEAKNGFGEWQEGNVQTFDKNLFTDELSVGLVVS
                     FADKSKAELAGVVKEITDQQVTVDFNHPLASRDLLFEVDIISVRD"
     gene            complement(444..938)
                     /gene="lspA"
                     /locus_tag="PLY05_07650"
     CDS             complement(444..938)
                     /gene="lspA"
                     /locus_tag="PLY05_07650"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNC03214.1"
                     /translation="MSSLHLKNLTWLLLSIIIIVIDQVTKLYFNQKLQLGQSIELLPV
                     LNWTLAYNYGAAFSFLHDAGGWQRWFFAGIAVVVSLGLTIWLVKLEKNAKFLAFAIAL
                     LLGGAIGNLYDRMTYGYVIDFIHVFYQTWHFPIFNFADCAITVGAIMLMIDSLFFEQK
                     RQVS"
     gene            complement(1078..3897)
                     /gene="ileS"
                     /locus_tag="PLY05_07655"
     CDS             complement(1078..3897)
                     /gene="ileS"
                     /locus_tag="PLY05_07655"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011279671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNC03215.1"
                     /translation="MTVDYKSTLNLPETAFAMKADLAKREPNWVKDWQENKLYQKIRQ
                     ARAGRPQFILHDGPPYANGNIHIGHSVNKILKDIIIKSKTLAGFDAPYVPGWDCHGLP
                     IELNVEKKFGKAGHKVSAKEFRQHCRDFAIGHVENQKADFQRLGVLGDWDNPYLTMNF
                     QQEADIIRSLGKIAANGHLHQGLKPVHWCMDCGSALAEAEVEYEDKQSHAVDVAFAVQ
                     DVADFAKRANLTNVPAAVDFVIWTTTPWTLPANQAVSLHPELVYVVVQATIEQQTRVL
                     VLAQALYEATLARYQAEDIQVLAEVAGAAFEHVALQHPFYERTVPVIVGEHVTADAGT
                     GAVHTAPGHGHDDFVVGKKYNLKVDNPVDNNGVFLPNTPLFAGMHVNKANEPIIETLK
                     ANNRLLAHVKIKHSYPHCWRHKTPIIFRATPQWFISMEQKGLRDAAMQAIDEVQWLPN
                     WGKARIVSMMQGRPDWCISRQRTWGVPIALFVHKITGELHPQTIDLIEKVALEVEKAG
                     IDAWWDIDAAVLLGAEAENYLKVTDTLDVWFDSGVTHSCVLTRPDLRYPADLYLEGSD
                     QHRGWFQSSLLTALAANGDKPFKTVLTHGFTVDEKGHKMSKSLGNVVSPQDIMKTLGA
                     DIIRLWVAATDYSGEMSVSKVILERMSDAYRRIRNTMRFLLANLAGFDPAQHLVPTND
                     MLALDRWVVDKAAQVQTDIIAAYEAYEFHNIYHKLHNFCSLDLGSFYLDIIKDRQYTT
                     QADSLARRSAQTALYHIIQALVRWCAPILSFTAHEIWQEIPQAGAESVFLTEWYQGLA
                     TLPEDDSQPMLTRAYWQQVMAVKTAVNKEIEEARTRKEVGSGLSAEVTLYVTPELKAR
                     LALLADELRFVLITSSATLSDQNGEGQVTDVVGLNVKVTPSTYTKCVRCWHYRADVGS
                     HAEHPEVCARCVENVAGQGEIRHFA"
BASE COUNT         1164 a          898 c          741 g         1094 t
ORIGIN      
        1 atcgcgaaca ctaatgatgt cgacttcaaa aagcaagtct ctactggcta aagggtgatt
       61 aaaatccaca gtgacctgtt gatctgtgat ttcttttact acgcctgcta gctctgcctt
      121 gcttttatca gcaaacgaga caactaagcc aacacttaat tcgtcagtaa ataaattttt
      181 atcaaacgtt tggacattgc cttcttgcca ttcgccaaaa ccatttttag cttcaataaa
      241 aatcgagcgt ttatcacctg cttttaggcc aaagatggcc ttttcaaagc cttctaataa
      301 actgccatca cctacggtaa aactgacagg ctgttcacgc tcacgtgtgg tatctaagag
      361 ttggccattg gccaaacaca ccgcaaaatg caaggtaact actttgtttg ggccaatacg
      421 ttgaattacg tcgtctttca ttatcaagat acctgtcgtt tttgctcaaa aaataaagaa
      481 tcaatcatta acatgattgc acccactgtg atggcacaat cagcaaaatt aaaaatgggg
      541 aaatgccatg tttgataaaa cacatgaata aaatcaatca cataaccata agtcatacgg
      601 tcatataaat tcccaattgc tccgcctaat aataaggcaa tggcaaaggc taaaaatttg
      661 gcattttttt ctagttttac tagccaaatg gtcaaaccta aactcaccac aacggctatg
      721 cctgcaaaaa accaacgctg ccagccacct gcgtcatgta aaaagctaaa tgctgcacca
      781 tagttataag ccaaagtcca atttaaaaca ggtagcagct caatcgactg gcctaactgt
      841 aatttttggt taaaatatag tttcgtcact tggtcgataa cgataatgat aatgcttaac
      901 aacaaccaag ttaagttttt taaatgcaaa gatgacattt actgttctct aaagtcaaaa
      961 ctagctgtta ctccctcacc ccaaccctct cccacagggc gagggagttt catcgctaaa
     1021 gtcttattaa atcggacgta ttacaacata aagccctcac atagagggct ttattgatta
     1081 ggcaaaatgt cgaatttcgc cctgccctgc aacgttttct acgcaacgag cacaaacttc
     1141 tggatgttcg gcatgactac ccacatcggc acggtaatgc caacagcgca cacacttggt
     1201 gtatgttgat ggggtcactt taacgtttaa gcctactaca tcggttactt gtccttcgcc
     1261 attttggtcg gacaaagttg cgcttgaggt aattaacaca aaacgcaatt catcggccaa
     1321 taaagctaaa cgcgctttta attcaggggt aacatataaa gtcacttcgg cagataaacc
     1381 cgaacccact tctttgcgag tacgtgcttc ttcgatttct ttattaaccg ctgtttttac
     1441 ggccatgact tgttgccagt aagcacgagt caacatgggt tggctgtcgt cttcgggtaa
     1501 agtcgctaaa ccttgatacc attcggtcaa aaatactgat tccgcacctg cttgtggaat
     1561 ttcttgccaa atttcgtgcg cggtaaaact caaaataggc gcacaccagc gtactaaagc
     1621 ttgaatgata tgatacaagg cagtttgggc tgaacgacgc gctaaagaat cagcctgtgt
     1681 ggtgtactga cggtctttaa taatgtctag gtaaaaacta cctaaatcta aagaacagaa
     1741 gttatgtagc ttatggtaaa tattatgaaa ttcatacgct tcataagcgg caataatgtc
     1801 ggtttgcacc tgtgctgctt tatcaactac ccaacgatct aaagccaaca tgtcattggt
     1861 tggcacaaga tgttgtgcag gatcaaaacc tgctaagttg gctaataaga agcgcatggt
     1921 gttacgaata cggcgataag catcactcat tcgttctaaa atgactttag aaacagacat
     1981 ttcaccactg taatcggtcg ctgccaccca taaacgaata atgtctgcac caagcgtttt
     2041 cataatgtct tgtggagaca caacattacc caaggattta gacattttgt ggcctttttc
     2101 atccaccgta aaaccatgcg ttaataccgt tttaaatggc ttatcaccat tcgcggctaa
     2161 agcggttaat aaagacgatt gaaaccaacc acgatgttgg tctgaacctt ctaaatataa
     2221 atcggcagga taacgcaaat ctggacgagt taagacacaa gaatgtgtta caccagaatc
     2281 aaaccatacg tctaaggtat cagtgacttt taggtagttt tctgcttctg cacccaataa
     2341 aaccgccgca tcaatatccc accatgcgtc aatacctgct ttttcaactt caagcgcaac
     2401 cttctcgatc aagtcgatgg tttgtgggtg taactcgcct gtgattttat ggacaaataa
     2461 cgcaattggt acaccccacg tccgttgacg cgaaatacac caatcagggc ggccttgcat
     2521 catagataca atacgtgcct taccccaatt gggtaaccat tgtacttcgt caatcgcttg
     2581 catcgctgca tcacgtaagc ctttttgttc catgctaata aaccattgtg gcgttgcacg
     2641 gaaaataatg ggtgttttat gacgccagca atgggggtaa ctgtgtttga ttttaacgtg
     2701 cgctaataaa cggttattgg cttttagcgt ttcaatgatt ggctcattgg ccttgttaac
     2761 gtgcatacct gcaaacaagg gtgtatttgg caaaaacacg ccgttattat caacagggtt
     2821 atcaactttt aagttatatt ttttgcccac cacaaagtca tcatgaccat gaccaggtgc
     2881 cgtatgtacc gcgcctgtcc ctgcatcagc cgtcacatgt tcaccaacaa tcacaggcac
     2941 agtacgttca taaaatggat gttgtaacgc aacatgctca aaagctgcac ctgcgacttc
     3001 agccaacact tgaatatctt ctgcttgata acgtgccaat gtcgcttcat acaaggcttg
     3061 agctaacact aaaacacgtg tttgttgctc aatggtcgct tgaacgacta catacactaa
     3121 ttcaggatgc aaactgaccg cttggttagc aggcaacgtc caaggtgtgg ttgtccaaat
     3181 aacaaaatcc accgcagcag gtacgttggt caaattggca cgtttcgcaa agtctgcaac
     3241 atcttgtaca gcaaatgcga catcaaccgc atgagattgt ttatcttcat attcaacttc
     3301 ggcttcggcc aaggcagagc cacaatccat acaccaatgc acaggtttta aaccttgatg
     3361 caaatggcca ttggcggcaa ttttgcctaa gctacgaata atgtccgctt cttgttgaaa
     3421 attcatggtc aagtaaggat tatcccaatc ccccaacacg cctaaacgct gaaagtcagc
     3481 tttttggttt tcaacatggc caatggcaaa gtcacgacaa tgttgacgaa actctttggc
     3541 agacacttta tgccctgctt taccaaattt cttttcgaca ttaagttcga taggcaggcc
     3601 atgacaatcc caacctggaa catagggcgc gtcaaagcct gctaaggttt tggatttgat
     3661 aataatatct ttgagaattt tattaaccga atgaccaata tgaatattac cattggcata
     3721 gggaggacca tcatgcaaaa taaattgtgg acgaccagca cgtgcttgac gaatcttttg
     3781 atataacttg ttttcttgcc aatcctttac ccaatttggc tcacgcttgg ctaaatcagc
     3841 tttcatcgca aaggccgttt cgggcaaatt tagcgtgctt ttataatcaa cagtcat
//
LOCUS       DAOQMB010000213         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11821_length_3891_cov_5.795360, whole genome shotgun sequence.
ACCESSION   DAOQMB010000213 DAOQMB010000000
VERSION     DAOQMB010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11821_length_3891_cov_5.795360"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1984
                     /locus_tag="PLY05_07660"
     CDS             <1..1984
                     /locus_tag="PLY05_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03216.1"
                     /translation="QYLKYKLKRWQTWSNNKRQQFAANTACQLAQVSFNDHHPNSLRH
                     LPTHHHWQILIDETGSEFDNSKHLSVHDTKLGRMVALAIPVNKVNLPPLKNNFHATNE
                     SDAILDATIKTLLDNRVGIVGITVNDALLGKSSRWFSGIYLLMRMVLRLLPINGQGKV
                     EFFIEQRSSFDANMSLFPIQQLLETELQALDAERFKHVSIALQFAHKNHHPANGYVDA
                     LAHLWAAGSANSKTRLKRSALRGHCLLEPQHDVIERSYAAIDQQHILSPKDWYVLVSA
                     VSNEPSSSLLHNVLAELGKHIQQQTTIWQGYLAFVNQLLQHKNYHLLELKTALDWLAN
                     YTPKECALPPMLTLHWYMARLACANHLGQHDLPLLSQSLTLAQGLLDENAMEVCHLHL
                     RIAVAATNQFEFDSAQDILDFWQSQDPRLMGLNNYGKYLSSLGQLAAFRGQNNQAISY
                     FDQAIRTFDQLSDPQEKRKQQRQTEIYRLIALTDNEGVSDQALQQALEAFWQKPLASI
                     ATLLGRSDNDQRFEQHLLLRVMVTRPALQQDSYLETASDWQFEQTHPWPLIAAYRAVL
                     LQTKQPIKASLWMHKAINTCREAQGATLQWMGEILALWVQQYGIQIDALPSKSLQQLQ
                     TQLPHAPWTSLEKLKNASNRSQAMQGLIACLPFNFH"
     gene            1996..3213
                     /locus_tag="PLY05_07665"
     CDS             1996..3213
                     /locus_tag="PLY05_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03217.1"
                     /translation="MDLYVLIVSDHNLAELRACLYFKPRYIWLVASQRMQEPAKRLLK
                     VLQAQCPSAQLDILRAMPDIPFEGNTLEETAAWIKQVFAPKINQFNNAIQQQCVLNMT
                     GGTKTLSYLLTRAYEWLEIHYQPFGNKVPLERFCLSAQGLNILPNIDLANVTISPKDN
                     ALLYMDFVRSHTPNPIRKHSNSLTVALSRLEQNQEWITLTKALEQGWSKNESGHFVSV
                     AIDADNTSTLLAQIKALNGLFNNSQHPQPLTLQNNTLSMPSFSHKPYEKWRKWVSGDW
                     YEQLIEHWLKTEYHIKPEYILTNVQLSNKTDPQGQESDTLLQFNNKLYVIEIKADIPK
                     YKTLGEMENQLTSLSGQLGKVENVLIVSPMLKDRYSNEQWLNFELRCKNKNVKLVVVA
                     TKQDLGVILNGHH"
     gene            3310..3630
                     /locus_tag="PLY05_07670"
     CDS             3310..3630
                     /locus_tag="PLY05_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNC03218.1"
                     /translation="MTTYTENSFWNKIKRVFKAAGREVIEKALWLHYAAQDPNVPLAA
                     KTTIYTALAYFILPVDLIPDFLPMGYTDDFGVLITAVSTVSMYITDEVKQKANDTLRQ
                     WFGS"
BASE COUNT         1222 a          825 c          733 g         1111 t
ORIGIN      
        1 ccaatactta aaatacaaac tcaaaagatg gcaaacatgg agcaacaata aacgccaaca
       61 atttgctgcc aacaccgctt gtcaattagc acaagttagc tttaacgacc atcaccccaa
      121 cagcctacgt catttgccta cacatcatca ttggcaaatt ttgattgacg aaacaggcag
      181 cgagtttgat aacagcaaac atctgagtgt acacgatacc aaattagggc gtatggttgc
      241 cttagccatt cctgtcaaca aagtaaattt acctccctta aaaaataact ttcatgccac
      301 caatgaatcc gacgcgatct tagatgcaac catcaaaacc ttgcttgata atcgcgtagg
      361 tattgttggc attaccgtta atgatgcttt attgggtaaa agctcaagat ggtttagtgg
      421 tatatatttg ttaatgcgta tggtgttacg tttattgccc attaatggtc aaggtaaagt
      481 tgagtttttt attgagcaac gtagtagctt tgatgccaat atgtctctct ttcctattca
      541 acaactactc gaaaccgaac tccaagcctt agatgccgag cgatttaagc atgtgagtat
      601 tgcgctacag tttgctcata aaaaccatca tcctgccaac ggttatgttg atgcactcgc
      661 ccatttgtgg gcagctggct ctgccaatag caaaacacga ctcaaacgca gtgctttgcg
      721 tggtcattgt ttgttagagc cacaacacga tgtgattgag cgcagttatg ccgccattga
      781 ccaacaacat atccttagcc ccaaagattg gtatgtttta gtcagtgcag tgagcaatga
      841 gccaagcagt agtttattgc ataatgtctt ggctgagctg ggcaagcata tacaacagca
      901 aaccacgatt tggcagggat atttggcttt tgtaaatcaa ttactacaac acaaaaacta
      961 tcatttgctt gagcttaaaa cggcattaga ttggctagcc aactatacgc ccaaagaatg
     1021 tgctttaccg cctatgctca ctttgcattg gtatatggct cgcttagcat gtgcgaatca
     1081 tttaggccaa catgatctgc ctttgttgtc gcaatcatta accttggcac aaggcttact
     1141 tgatgaaaat gccatggaag tttgtcattt acatttgcgt attgcagtgg cagccaccaa
     1201 ccaatttgaa tttgactcgg cacaagacat tttagatttt tggcaatcac aagacccacg
     1261 tttaatggga ttaaataact acggcaaata tctgtctagc ttaggccagt tagcagcgtt
     1321 tagaggccaa aataatcaag cgattagcta ctttgaccaa gcgatacgca cttttgacca
     1381 attaagcgac ccacaagaaa aacgtaaaca acaacgtcaa accgaaatat accgattaat
     1441 tgcactcacc gacaacgaag gagtgtctga ccaagcctta caacaagcct tagaagcttt
     1501 ttggcaaaaa ccactagcaa gcattgccac gttgcttggc cgcagtgata acgaccaacg
     1561 ctttgaacaa cacttattgt tacgggttat ggtaacaaga ccagcattac aacaagatag
     1621 ctacttagaa accgcatccg attggcaatt tgaacaaaca cacccttggc cgttgattgc
     1681 cgcttatcgt gctgtgctat tacaaaccaa acagcccatt aaagcgagtt tgtggatgca
     1741 caaagccatc aatacttgtc gtgaggcaca aggagcaacc ttacaatgga tgggcgaaat
     1801 attggcatta tgggtgcaac aatatggcat tcaaatagac gcactgccaa gcaaatcctt
     1861 acagcaactg caaactcaac tgcctcatgc gccatggaca tctttagaaa aactaaaaaa
     1921 tgcgtctaac cgtagccaag ccatgcaagg cttaatagca tgtttaccgt ttaattttca
     1981 ctaaggaaca aaaaaatgga cttgtatgtg ttgattgtct ctgaccacaa cttagccgaa
     2041 ctacgcgcgt gtttatattt taaaccgcgt tatatttggc tagtggccag tcaacgtatg
     2101 caagaacctg ccaaacggtt attaaaagta ttacaagcac aatgcccaag cgcacagtta
     2161 gacattttac gagccatgcc tgatattcct tttgagggca acaccttaga agaaacagcc
     2221 gcttggatta aacaagtttt tgcgccaaaa attaatcaat ttaacaatgc tattcaacag
     2281 caatgtgtgc ttaatatgac gggcggcact aaaacattat cttatttact tacccgtgct
     2341 tatgaatggc tagaaattca ttaccaacct tttggcaata aggttccatt agagcgtttt
     2401 tgtttatctg ctcaaggctt aaacattttg ccaaatattg atttagccaa tgtgactatt
     2461 tctcccaaag ataacgcttt gttatatatg gactttgttc gctcacacac gcctaaccct
     2521 attcgtaagc actcaaacag tttaacggta gcactgtctc gacttgagca gaatcaagaa
     2581 tggataacac taactaaggc tttagaacaa ggttggtcta aaaatgaatc aggccatttt
     2641 gtttcagttg cgattgatgc cgacaataca agcacattgt tggcacagat taaagcgtta
     2701 aacggcttat ttaataacag tcaacatcca cagccattaa ccctgcaaaa taacaccttg
     2761 agtatgccct cttttagcca taagccttat gaaaagtggc gcaaatgggt gtcaggtgat
     2821 tggtatgaac aactcattga gcattggttg aagactgaat accacataaa gcctgagtat
     2881 attttaacta acgtacagtt aagcaataag actgacccac aaggtcaaga aagcgacacc
     2941 ttattgcaat ttaacaataa actttatgtg attgaaataa aagccgatat tcccaaatat
     3001 aaaacactag gcgagatgga aaatcagctt acatctttat cgggacaact tggtaaagta
     3061 gaaaacgtgt taattgtgtc gcctatgtta aaagacagat atagcaatga acaatggcta
     3121 aattttgagt tacgctgtaa aaacaaaaac gtaaaattag tcgttgttgc tacaaagcaa
     3181 gacttaggtg tgatactcaa cggccatcat tagcccgaat atacatcgta agcttgtaaa
     3241 gtctctgaca gtgaaagtac tgagctatgc tactcgtact taattacctc actgttacgg
     3301 agcattatca tgaccactta taccgaaaac tctttttgga acaaaattaa aagagttttt
     3361 aaagccgcag gccgtgaagt catcgaaaaa gcattgtggc ttcactatgc cgcgcaagac
     3421 cctaatgtac cattagctgc taaaaccacc atttacaccg ccctcgccta ttttattttg
     3481 cctgttgatt taattcccga ttttctgcct atgggttata ccgatgattt tggcgttctt
     3541 attacagccg ttagcaccgt atctatgtac attaccgatg aagttaaaca aaaagccaat
     3601 gacacactaa gacaatggtt tggaagttga ccaccgctta tattagtgca tgagtctgtt
     3661 agtcatcttt ccataagctt gcaatttata aatcgccatt tgttatcaca cataattgat
     3721 tttaactttt ttggttttta aggtaactca tcatgaatac agtttttttg atttctactt
     3781 ttgtggcttg tgttgttttt ttggttattg gtatggtgat tggtcgtagc ctttccaaaa
     3841 ccatgttatt gagcttagaa agccagttaa aaacccagtt tgatgccgag a
//
LOCUS       DAOQMB010000214         3888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11838_length_3888_cov_16.946778, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000214 DAOQMB010000000
VERSION     DAOQMB010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11838_length_3888_cov_16.946778"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLY05_07675"
     CDS             complement(<1..236)
                     /locus_tag="PLY05_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HNC03219.1"
                     /translation="MSKILLKWLNYLKPNFKVLSAKERGLSTLGALLGLMLSSLICWY
                     FLRGINPWYIAPMGASSVLLFAVSSSPLAQPWNML"
     gene            complement(306..2384)
                     /gene="glyS"
                     /locus_tag="PLY05_07680"
     CDS             complement(306..2384)
                     /gene="glyS"
                     /locus_tag="PLY05_07680"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNC03220.1"
                     /translation="MTTLPFLVELGCEELPPKSLKTLAESFLAGIEAGLKQAGLSYDH
                     ARYYAAPRRLAVSVQHLALQQPDRTVHVDGPPVKAAFDANGNPTQAAVGFAKKNGVDI
                     SEVDRSGEKLRVSKHIQGETAVALLPQIVQQALDNLPIAKRMRWGASKVEFVRPTQWL
                     VMLLGDKVVPTTILSQQADRITRGHRFMAQDERLALHYTNYPASLEHSYETQLEMAYV
                     VPDFAKRQQMIVAAVEKLAAEQGGKALMPAALLDEVTALVEYPVPLVCRFEERFLHVP
                     QEALISTMQDNQKYFCLVDNNGKLLPRFITVANVKSTNAKFIVEGNEKVVRPRLTDAE
                     FFFKQDKKVTLESRNERLKTVVFQAQLGTVYEKAERISELAAFIAAQIGGDVALAQRA
                     GILAKCDLATEMVGEFPELQGIAGYYYAQNDGEANDVALALNEQYLPKFAGDVLPSTN
                     TGLAVAIADKLDTLVGIFGINQPPTGSKDPFGLRRAAIGLLRIIIEKELALDINVLSQ
                     QAVNAYGSKLSNANTVNEVFEFLLGRYRAMYEEQGIAVDVIQAVQTLKPAQPLDFDRR
                     IKAVANFRNLAEAEALAAANKRVANILSKDGVTVTGGVDNSLLSEAAEQALAAQVASL
                     QTQLAPLFAAGDYTPALSALAALRAPIDQFFDSVMVNAEDAAIRQNRLNLLASLRELF
                     LKTADISQLQ"
     gene            complement(2384..3346)
                     /gene="glyQ"
                     /locus_tag="PLY05_07685"
     CDS             complement(2384..3346)
                     /gene="glyQ"
                     /locus_tag="PLY05_07685"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNC03221.1"
                     /translation="MNSNAIPQAINTFQGLILALQNYWAQQGCVVLQPYDMEMGAGTF
                     HTATFLRALGPETWNAAYVQPSRRPADGRYGENPNRLQHYYQFQVVLKPNPDNIQELY
                     LNSLRHIGIDPTVHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGVEC
                     YPVTGEITYGLERLAMYLQGVDSVYDLTWTDGQFGKVTYGDVFHQNEVEQSTFNFEHA
                     NVEKLFDLFDFYESEANRLMALPQPLPLPAYEMVIKASHSFNLLDARRAISVTERQRY
                     ILRVRQLARAVAQSYVQARARLGFPMASIELRDEVLAKLAAESK"
     gene            3615..>3888
                     /locus_tag="PLY05_07690"
     CDS             3615..>3888
                     /locus_tag="PLY05_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03222.1"
                     /translation="MSMPHYLINNKLKPLVVALSVVVVTPIYANDSLWDMSLEDLGKI
                     RVTTLASGTATPLDKAAAIATVITAEDIEAMGANDISQVLETVPDLH"
BASE COUNT         1152 a          888 c          765 g         1083 t
ORIGIN      
        1 agcatattcc acggttgtgc taagggacta cttgatacag caaataacaa aaccgatgac
       61 gcgcccatgg gagcaatata ccaaggattt atgcccctta aaaaatacca acaaataagc
      121 gatgaaagca ttaaaccaag taatgcacct agcgtactca aacctcgctc tttagcggac
      181 aaaaccttaa aattaggctt taaataatta agccatttaa gtaatatttt tgacacaact
      241 caaccaatac attgaattaa aaagaaaaac ggctcaactt gagccgtttt atggttcata
      301 atccgttact gcaactgcga aatatccgcc gtcttcaaaa acaattcacg caaactcgcc
      361 aataaattta agcgattttg acgaatggct gcatcttccg cattcaccat taccgaatca
      421 aaaaactggt caatcggtgc gcgtaaggcc gccaaagcag ataacgctgg tgtgtaatca
      481 cctgcggcaa ataatggggc taattgtgtt tgtaagctcg ccacttgggc ggctaaggct
      541 tgttcagccg cttcgcttaa caaactatta tccacaccac ccgtcaccgt cacgccatct
      601 ttgcttaaaa tattggccac acgtttatta gcagccgcta aagcttcagc ttcggctaag
      661 ttgcggaagt tcgctaccgc tttaatacga cggtcaaaat ctaaaggttg agcgggcttg
      721 agcgtttgta ccgcttgaat gacatcaacg gcaatgcctt gttcttcgta catagcacga
      781 taacgaccta ataagaactc aaatacctca ttgacggtat tcgcattgct gagtttgctg
      841 ccataggcat tgaccgcttg ttgactcaag acattaatat ctaaggctaa ttctttttcg
      901 ataataatgc gtaacaaacc aatcgccgca cgacgtaaac caaacgggtc tttgctgcct
      961 gtgggtggtt gattgatgcc aaaaataccc actaaggtat cgagtttgtc agcaatggca
     1021 accgctaaac ctgtgttggt gctaggtaaa acatcacctg caaatttagg taaatattgc
     1081 tcatttaacg ccaaagcgac atcgttggct tcaccatcgt tttgggcata ataataacca
     1141 gcaatacctt gtaactcagg aaactcgcca accatttcgg tggctaaatc acacttcgct
     1201 aaaatgcctg cacgttgtgc caatgccaca tcaccgccaa tttgggcggc aataaatgcg
     1261 gccaattcgc taatacgttc ggctttttcg tacactgtgc ctaattgggc ttggaaaacc
     1321 acggttttta agcgttcatt acgactttca agtgttactt ttttgtcttg tttaaagaag
     1381 aattcggcat cggttaaacg tgggcgcacc actttttcgt tgccttcaac aataaacttg
     1441 gcattggtgc ttttgacatt agcaacagta ataaaacgtg gcaataattt gccgttgtta
     1501 tcaaccaaac aaaaatactt ttggttatct tgcatggttg aaatcaaggc ttcttgtgga
     1561 acgtgcaaaa agcgttcttc aaaacgacac accaacggca ctggatattc caccaaggcg
     1621 gtgacttcgt ccaataacgc cgcaggcatt aaggctttgc cgccctgctc agccgctaac
     1681 ttttctaccg ccgccacaat catctgttga cgtttggcaa aatcaggcac aacgtaagcc
     1741 atttctaatt gggtttcgta gctatgctct aagctggctg gataattggt gtaatgaagc
     1801 gctaaacgtt catcttgtgc cataaaccga tgaccacggg taatacggtc agcttgttga
     1861 cttaaaatag tcgttggtac aaccttgtca cccaacaaca tcaccaacca ttgggttgga
     1921 cggacaaatt caactttaga cgctccccaa cgcatacgct tagcaattgg caagttatct
     1981 aaggcttgtt gcacaatttg tggcaacaaa gcaacggctg tttcaccttg aatatgcttg
     2041 ctgacccgta atttttcgcc gctacggtca acttcgctaa tatccacacc attctttttg
     2101 gcaaaaccta cagccgcttg ggtgggattg ccattggcat caaacgctgc tttcacaggt
     2161 gggccatcaa catgcacagt gcggtctggt tgttgcaagg ctaaatgttg cacgctcact
     2221 gctaaacgac gtggtgcagc ataataacgt gcatggtcat aacttaaacc cgcttgtttt
     2281 aagcctgctt caatgcctgc taaaaaactt tctgctaaag tttttaagga ttttggtggt
     2341 aactcttcac agcctaattc aactaaaaaa ggtaaagtgg tcattatttg ctctccgctg
     2401 ccaattttgc caagacttca tcacgtaatt cgatgctcgc cattggaaag cctaaacgag
     2461 cacgcgcttg cacataactt tgtgctaccg cacgtgctaa ttgacggaca cgcaaaatat
     2521 aacgctgacg ctcggtgacc gaaatcgcac gacgcgcatc taataagtta aagctatgag
     2581 aggctttaat caccatttca taagcaggca acggtaaggg ttgtggtaaa gccattaaac
     2641 ggttggcttc actttcgtaa aaatcgaata aatcaaatag tttttcgaca ttggcatgtt
     2701 caaagttaaa ggttgattgt tcaacttcat tttgatggaa cacatcacca taggtcactt
     2761 taccaaattg gccatctgtc catgttaagt cataaacaga atccacacct tgcaaataca
     2821 tcgctagacg ttctaaacca taggtaattt cgcccgtcac aggataacac tctacaccac
     2881 ccacttgttg aaagtaggta aattgagtga cttccatgcc gtttaaccac acttcccaac
     2941 ctaagcccca tgcacctaat gtcggagatt cccagttatc ttcgacaaaa cgaatatcat
     3001 gcacggtggg gtcaatgcca atatgacgca aagaatttaa atataactct tgaatattat
     3061 ctggattggg ttttaacacc acttgaaatt gataatagtg ttgcaagcgg ttggggtttt
     3121 cgccataacg accatccgca ggacgacgtg acggctgcac ataggcggcg ttccatgttt
     3181 ctgggcctaa agcacgtaag aaagttgcgg tatgaaaagt acctgcaccc atttccatat
     3241 cgtagggttg taacacgaca cagccttgct gcgcccaata attttgtaag gccaaaatca
     3301 atccttgaaa ggtattgatg gcttgaggaa ttgcattgct gttcattgtt atctgatgac
     3361 taagaataaa aaagtgacct agtataatcg cggcttaggt gtagggctag acagtgtttg
     3421 attttattaa acataaatga cacgctagac cactgcttaa aaaacaatct aactttgatt
     3481 gaccaacgat agccattagg cttaacttaa caatgacgct aaaaatgtat accaaatcac
     3541 tttttaccgc ctaaaacaac aaactctaag catttatacc tgttaatcaa aaaaaatcta
     3601 ttaggttaat catcatgtct atgcctcact atttaattaa taataaactt aaacctttag
     3661 ttgttgctct ttctgttgtt gtagtgacac caatttatgc taatgacagt ttatgggata
     3721 tgtctttaga agatttaggc aaaattcgag tcaccaccct tgcatcgggt acagcaaccc
     3781 cgcttgataa agcagctgct attgcaaccg ttattactgc tgaggatatt gaggcaatgg
     3841 gtgccaatga tattagtcaa gttttagaga cagtgcctga tcttcatg
//
LOCUS       DAOQMB010000215         3878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11903_length_3878_cov_8.560555, whole genome shotgun sequence.
ACCESSION   DAOQMB010000215 DAOQMB010000000
VERSION     DAOQMB010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11903_length_3878_cov_8.560555"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLY05_07695"
     CDS             <1..571
                     /locus_tag="PLY05_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC03223.1"
                     /translation="GGIAAALYLRSKKQYDKALNILEMIANYLSNSQMRTRLLVVRAN
                     QIVIWSLQGQHSFAKQQLVELFNNVGLQCAIKTIFDEAPSFGELLRDAHEEGLICLPD
                     IYLQLYHDVLYPSQKPVTLPHKPSLEELTAKEYEVLALMQKGCSNKEIGDVLNISLST
                     TKWHIKNIFTKLNVANRSAAVSLSFNRRL"
     gene            complement(688..1770)
                     /locus_tag="PLY05_07700"
     CDS             complement(688..1770)
                     /locus_tag="PLY05_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HNC03224.1"
                     /translation="MADIRSLLTMMLQQGASDMILTAGYKPYIKVNGVMHIAYAPSLA
                     QGETATLALELLSPQQRQAFSEQKEANLAVSFDGIGRFRVNLYYQKGQISIAFRHIKD
                     HVLSFEQLGVPKQAIDFAMLKRGLVLITGMAGSGKSTTLAAMIEHRAKHLTGHIITIE
                     DPIEYILNHSRALVEQREIGFDTLSFNEALRNVLRESVDVIVIGEIRDYETAKQAVAY
                     ADGGILCLATMHANSADQAVSRLLNFFPMDMREQVLLDLSLNLKGILCQRLLPSQGKQ
                     VLASEVMVQSAYLSDLIVAGKLYQLKDAMRKSTEEGMQIFDESLFRLYKAGLITLDEA
                     MHNADSQADLSVRVRLASWDFPLQLL"
     gene            1861..2097
                     /locus_tag="PLY05_07705"
     CDS             1861..2097
                     /locus_tag="PLY05_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03225.1"
                     /translation="MASNVNYANKIPYLDTITDSIGLRNYYQHYHQVTSHSAPQLLLE
                     SGLIKQQTLNEILSASDYKQDQHLGFNLYQKKTD"
     gene            2093..3631
                     /locus_tag="PLY05_07710"
     CDS             2093..3631
                     /locus_tag="PLY05_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HNC03226.1"
                     /translation="MNDEDLGHLLGACFGLPFVYLHHFDVDLNAVQILPADFARKHHV
                     MPLLFLENHKLVIAVFDPTDNQLLENLQFISGHIVELAVATLDDILLSISTYYSEQEM
                     AQALANVEGLIKATPEINSDNPINEKILERPVVKLGQNLITDAIVSRASDIHIRPTQH
                     SVDILFRIDGMLAHQRSLNKQLQHIITTRIKILGGMDISERRLPQDGRSKIHFAEKDV
                     DLRLSVIPSIYGEDVVIRLLDTQFAMQHLEDLGYEGDDANRIKNVLSRNNGMFLITGP
                     TGSGKSTTLYTVLEQIRNKNINIITIEDPVEYHIDGIMQIQVHPQIGYSFARALKHIL
                     RHDPDVIMVGEIRDEETAKMAVESALTGHLMLSTLHTNSAATTITRFLEIGIEPYMLA
                     STLLGVLAQRLARCNCQHCLVVEEVDSSIRQVMGASTDEVFYKGAGCEICKHRGVKGR
                     RAVYELLIVNSEIRQNIHHDINADLLQQIAIKEGMTPLTQHALHLARQKIISLSEAYR
                     VRLD"
     gene            complement(3591..>3878)
                     /locus_tag="PLY05_07715"
     CDS             complement(3591..>3878)
                     /locus_tag="PLY05_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03227.1"
                     /translation="SLHLSFDLPFAQALVIDYVLYLPRANGKHSQKVFKWKTAKFSQG
                     QQQLTTNFSFKEITTRRYYAGEHRFVVLVNGQERAEASINLNVLDKPLTTK"
BASE COUNT         1241 a          741 c          664 g         1232 t
ORIGIN      
        1 tggtgggatt gccgctgcct tatacttacg cagcaaaaaa caatatgaca aagcccttaa
       61 cattctggag atgattgcta attatttaag caatagtcaa atgcgtactc gtttgcttgt
      121 ggttagagcc aatcaaattg tcatttggtc attacaaggt caacatagtt ttgccaaaca
      181 acagttggtt gaattattta ataatgttgg cttgcaatgt gcgattaaaa ccatttttga
      241 tgaagccccc tcctttggtg aattgttgcg tgatgcacat gaagaaggtt taatttgcct
      301 gcctgatatt tatttacagt tatatcatga tgtcctttat ccaagccaaa aacccgtcac
      361 actccctcat aaacctagct tggaggagtt aacagctaaa gaatatgagg tcttagcttt
      421 gatgcaaaaa ggttgtagca ataaagaaat aggtgatgta ctaaatattt ctttatcaac
      481 aaccaaatgg catatcaaaa atatttttac caaattaaat gtggctaatc gttcagcagc
      541 ggtatctttg tcatttaatc gaaggttgta ggtttacatg catattaaga cttctctatt
      601 atcgtttatt tgcgcacact tcaattgttc taaacgattt tatgttcata aaacacacta
      661 atttcagcga agtgcgtaag tccctattta taacaactgc aatggaaaat cccatgaggc
      721 taaacgcact cgtactgata aatcagcttg agagtcagca ttatgcatag cctcatctaa
      781 ggtaatgagt cctgctttat agagtctaaa caaagattca tcaaaaattt gcataccttc
      841 ctctgtactt ttgcgcatgg catcttttaa ttgatataat ttacctgcaa cgattaaatc
      901 agataaataa gctgactgaa ccatcacttc gcttgctaac acttgcttac cttgagaagg
      961 taataaacgc tgacataaaa taccttttaa attaagagat aaatctaata atacttgctc
     1021 acgcatatcc ataggaaaga aatttaataa tcgactgact gcttgatctg cactattagc
     1081 atgcattgtt gctaaacaca aaataccgcc atctgcataa gcaactgcct gtttagccgt
     1141 ttcataatct ctaatttcac caatgactat aacatccact gactctcgta aaacattacg
     1201 taaagcctca ttaaaactta aagtatcaaa gccaatctct cgttgctcaa ctaatgctct
     1261 actatggttt aaaatatatt ctatcgggtc ttcaatagta ataatatggc ctgttagatg
     1321 tttagcccta tgttctatca tggctgctaa agtggtactt tttcctgagc ctgccatgcc
     1381 tgtaattaat accaaacctc ttttcagcat tgcaaaatca atggcttgtt ttggcacacc
     1441 taattgttca aatgacaaaa catgatcttt gatatgtctg aatgcaatgg aaatttgccc
     1501 tttttgataa tataaattta ccctaaaacg accaatacca tcaaaagata cagctaaatt
     1561 tgcttctttt tgctcactaa acgcttgtct ttgctgtggt gataataact ctaatgctaa
     1621 ggtcgccgtt tcaccttgtg ccagagaagg agcataagca atatgcatca cgccattaac
     1681 tttaatatag ggcttatagc ccgccgtcaa aatcatatct gatgcgcctt gttgcagcat
     1741 catcgtcaac agactacgaa tatctgccat tcttccccct ccttatattg caaggcttga
     1801 cgctagagca gaagctactt atgctcataa aaactacttt tcttttttgt gtaaatcatt
     1861 atggcaagca acgttaacta tgccaataaa attccgtatt tagatacaat tacagatagt
     1921 attggtttac gtaactacta tcagcattac catcaagtta catcacattc tgccccacaa
     1981 ttattattag aatcggggtt aattaaacag caaactttaa acgaaatttt atctgcttcg
     2041 gactataaac aagatcaaca tttaggtttt aatttatatc aaaaaaaaac tgattaatga
     2101 cgaagattta ggccatttat taggtgcttg ttttggctta ccttttgtat atttgcatca
     2161 ttttgatgtt gatctaaatg ctgtgcaaat cttacctgct gactttgctc gtaaacatca
     2221 tgtgatgcca ttattatttt tagaaaatca taaactagtg attgcagttt ttgatcccac
     2281 ggataatcaa cttttagaaa atctacaatt tattagtggt catattgttg aattagccgt
     2341 cgcaacacta gatgatatct tattatctat atcgacctat tatagtgaac aagaaatggc
     2401 tcaggcacta gccaatgttg aaggtttaat taaagctacc cccgaaatta atagtgataa
     2461 tccaattaac gaaaaaatat tagaaagacc tgttgttaaa ttaggtcaaa acttaattac
     2521 cgatgctatt gtcagtcgcg catctgacat tcatattcgt ccaactcaac atagtgttga
     2581 tattttattt agaattgatg gcatgctagc gcatcaacgc agcttaaata aacaactaca
     2641 gcatattatt acaacacgca tcaaaatttt aggcggcatg gatatttctg agcgtcgctt
     2701 accccaagat ggccgttcaa aaatacattt tgcagaaaaa gatgtagatt tacggttgtc
     2761 ggttattcct agtatttatg gtgaagatgt cgtcattcga ctgttagaca cacaatttgc
     2821 tatgcaacac ttagaggact taggttatga gggtgacgat gcgaatcgaa ttaaaaatgt
     2881 actaagccgt aataacggca tgtttttaat tacaggacca acaggatcag gaaagtcaac
     2941 aacactctat acggtacttg agcaaatccg taataagaac ataaatatca tcaccataga
     3001 agaccctgtc gaataccata ttgatggtat tatgcaaatt caggttcatc cccaaattgg
     3061 ttatagtttt gcacgcgcat taaaacacat tttaagacat gaccctgatg tgattatggt
     3121 gggtgagatt cgtgatgaag aaacagccaa aatggcggtc gaaagtgcgt taacaggcca
     3181 tttaatgtta agtaccttac ataccaacag tgcagcaacc acaattaccc gctttttaga
     3241 aattggtatt gagccttata tgttggctag taccttactt ggtgtattgg cacagcgttt
     3301 agcacgttgt aattgccaac attgcttagt tgttgaagaa gttgactcca gtattcggca
     3361 agtcatgggt gcaagtactg atgaagtctt ttataaaggt gcaggctgcg aaatatgtaa
     3421 acatcgtgga gtaaaaggcc gccgagcagt ttatgagtta ttgattgtta atagtgaaat
     3481 tcgtcaaaat attcatcatg atattaatgc cgatctttta caacaaattg caattaaaga
     3541 aggcatgacc ccactcactc aacatgccct acatttagcg cggcaaaaaa tcatttcgtt
     3601 gtcagaggct tatcgagtac gtttagatta atactggcct ctgccctttc ttgaccattc
     3661 accaacacca caaaacgatg ttcacctgca taataacggc gtgtggtaat ttctttaaaa
     3721 ctaaagtttg tggttaattg ctgttgtcct tggctaaatt tggctgtttt ccatttaaat
     3781 actttttgtg aatgtttacc attcgctcgc ggtaaatata aaacataatc aatcactaaa
     3841 gcctgagcaa atggcaagtc aaaagataaa tgtaagct
//
LOCUS       DAOQMB010000216         3872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11938_length_3872_cov_17.363112, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000216 DAOQMB010000000
VERSION     DAOQMB010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11938_length_3872_cov_17.363112"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="PLY05_07720"
     CDS             complement(<1..2135)
                     /locus_tag="PLY05_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC03228.1"
                     /translation="MTAYLFSTITPTPAGFIYTPEIFRKDLLSRVFAIYCQQPEPIIQ
                     LANHHIEQVKGLVIYPAEQCCLQQISPHFWKMGIPSMAEAHLYNLVNPLLTTLESLVH
                     SHDHNIDLQRRLKRINRDLEQVQYDYHRATHALAQQVQHLTTIENKLRHGEAKLKRII
                     DLLPQQIYAIDENNTILLVNQAYAQAHNLSIEQIIGQQTKDIAPPDDVASGWFKTAQE
                     ANYKVRQEQIRVDIPERPLATMNGQRIFHVTKIPFNEDNEHQLGVLTVATDITEHKQA
                     AQAIQKLNFELEARVAERTAALEQANLHLQQAKTQAESANEAKSLFLAMMSHEIRTPM
                     NGIVGMIDLLKETTLDHEQANMLSTIRESSFVLLNLLDDILDFSKIEAGRLSLERIPF
                     SLSELIESVTETLMPNALQKDISLSCFINPDLPDRLEGDPVRLRQILLNLCSNAIKFT
                     HPSTSKQGMVQIRADIHALHANAVELMLQVEDDGIGIAPDIIHQLFQPFSQAENSIRR
                     NYGGTGLGLSICRRLTEIMGGYIEVNSSVGIGSCFSVFVRLPIHPHTANPINLTQQKI
                     IAVLDETPLRKIIYSYLNAAHADVAIFSDVNQIDNSIINHQSSILLLDSRWPTPPTHL
                     AHLPTILLTHRHDKASLGHHLPDKQWYLWISPLRRDDLYQSILLASGVLKNTYAHPHQ
                     INNNRKQPLGIIEAEQQGALILVAEDNPV"
     gene            complement(2132..3259)
                     /locus_tag="PLY05_07725"
     CDS             complement(2132..3259)
                     /locus_tag="PLY05_07725"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HNC03229.1"
                     /translation="MEALSVSTQIPDAYRAGVYLGEHLAALNPEVVFLFTSIDYGHNS
                     DILAGLQDSLNQPNCIIIGNSGDGFYETHGVGEYGAAALALNSHGQVQWQLKIGHHVT
                     EQPQQATRTALQEHQQASLIFMISDFHADASLIEEVIEHEIKTPIVGGFAADDQRWDQ
                     CALYTCDGVINDCVIALHAIGNINFSIFVDNSITPIGHSGTIEQAESNRIDRIGGMDA
                     MRFIEQETGKPILRSDRGNISLTIINHENFKRLRSIKSDIGENAQSIFLYGGISTGQK
                     VQVCIARPDEIIHEVYQLAQQAQSASFQPIAALIISCAGRKSLLGTQLNHEIDAITKN
                     FQQISLIGFPSLGEIAPLRLKDGYSANLFHNMTYVLVLIGL"
     gene            complement(3382..>3872)
                     /locus_tag="PLY05_07730"
     CDS             complement(3382..>3872)
                     /locus_tag="PLY05_07730"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC03230.1"
                     /translation="MNSKVSSVSKIDNHVYKIFLNDLNAKRTVFGGLIMSIADRLAVV
                     VAERHSGHTCVTASVDSWHFVAPAQENDTLLFSAAINKAWTSSMEIGVRVEAENSYLG
                     TKRHIVSAYFTFVALDAEGKPAPVPALVPENEKEQQRFEAAEIRRQARLSVREQLKQY
                     KA"
BASE COUNT         1174 a          689 c          776 g         1233 t
ORIGIN      
        1 ttaacaggat tatcttctgc cactaaaatt aatgcgcctt gttgttcggc ttcaataata
       61 cctagcggtt gtttgcggtt attgttgatt tgatgaggat gagcgtaagt attttttaag
      121 acaccacttg ctaataaaat gctttggtac aaatcatcac gacgtaatgg tgaaatccat
      181 aaataccatt gtttatcagg taagtgatgc cccaagctgg ctttgtcgtg acgatgtgtt
      241 aataaaatag tgggtaaatg ggctaaatga gtgggtggag taggccaacg actatctagt
      301 agtaaaatac tggattgatg attaatgatt gagttatcta tttgattaac atcagagaaa
      361 attgctacat ctgcatgagc agcatttaaa taactataaa taattttgcg taaaggtgtt
      421 tcatctaaca ctgcaataat tttttgttgt gttaaattga taggattggc tgtgtgtgga
      481 tgaatgggca atcgtacaaa gacactaaaa caactgccaa taccaacaga actattaact
      541 tcaatataac cacccataat ttcagttaga cgacgacaaa tcgataatcc taaacctgtg
      601 ccaccataat tacgacggat agagttttcg gcttggctaa aaggctgaaa taattgatga
      661 ataatatcag gtgcaatacc aataccgtca tcttctactt gcaacataag ttctacggca
      721 ttggcatgta aggcatggat atcggctctg atttgaacca tgccttgttt acttgtactg
      781 ggatgagtaa atttaatggc attactacat aaattgagca atatttgtcg tagtctaaca
      841 ggatcgcctt ctagcctatc gggtaggtct gggttaataa aacagcttag agaaatatct
      901 ttttgtaagg cattaggcat gagtgtttca gtgacacttt ctatcagttc actcaatgaa
      961 aacggaattc tttctaggct taaacgacca gcttcgattt tagaaaagtc taaaatatca
     1021 tctaataaat taagcaatac gaatgaagat tcacgaatag tgctaagcat attggcttgt
     1081 tcatgatcaa gtgtcgtttc ttttaataaa tcaatcatgc caacaatgcc attcattggc
     1141 gtacgtattt cgtggctcat cattgccaaa aataaacttt tggcttcatt agcactttcg
     1201 gcttgtgttt ttgcttgttg taggtgcaaa ttggcttgtt ctagtgcggc agtgcgttca
     1261 gcaacccgtg cttctaattc aaaattgagt ttttgaattg cttgggcagc ttgtttatgt
     1321 tcagtgatat ctgtcgcaac ggttaatacc cctagctgat gttcattgtc ttcattaaaa
     1381 ggaatttttg tgacatgaaa aatacgctgg ccattcatgg ttgccagagg tctttcagga
     1441 atatcaacac gaatttgttc ttgacggact ttatagttag cttcttgagc ggttttaaac
     1501 caaccactag caacatcatc aggtggagca atatctttag tttgttgacc aataatttgt
     1561 tcaatactca agttatgtgc ttgagcatag gcctgattga ctaacaaaat cgtattgttt
     1621 tcatcaattg cataaatttg ttgtggtaat aaatcaatga ttctttttaa ttttgcttcg
     1681 ccatgacgca gtttgttttc gatagttgtt aaatgttgaa cttgttgagc taaagcatga
     1741 gtggcacgat gataatcata ttgaacttgt tctaaatcac gatttattcg ttttagtcgg
     1801 cgttgtaaat caatattgtg atcgtgcgag tgtactagac tttctaatgt agttaataaa
     1861 ggatttacta gattgtatag atgagcctca gccattgagg ggatgcccat tttccaaaaa
     1921 tggggactaa tttgttgcaa acaacattgt tcggcaggat aaatcactaa gcctttgact
     1981 tgctcgatat gatgattagc cagttggata atgggttctg gttgttgaca ataaatggca
     2041 aatactcgac ttagtaagtc tttacgaaaa atttcaggtg tataaataaa acctgctggg
     2101 gtgggagtga tggtactaaa aagataagca gtcataagcc aatcaacact aaaacataag
     2161 tcatattatg aaataagttt gcactataac cgtcttttag gcgtaagggg gcaatttctc
     2221 ctaatgatgg aaagccaatt aagctaattt gttgaaagtt tttggtaatt gcgtctattt
     2281 catgattgag ttgtgtgcct agtaggcttt ttcgacctgc acaactaata attaatgcgg
     2341 caattggttg aaaacttgcc gattgggctt gttgtgcaag ctgatagacc tcatgaatga
     2401 tttcatcagg acgagcaata caaacttgta ctttttgtcc tgtactgatc ccgccataca
     2461 aaaatatact ttgtgcattt tcaccaatat cacttttgat ggagcgtagg cgtttaaagt
     2521 tttcatggtt aataatagtt aatgaaatat ttccgcgatc actgcgtaaa atgggtttac
     2581 ctgtttcttg ttcaataaag cgcatggcat ccataccacc aatacgatca atacgattac
     2641 tttcagcttg ttcaattgtg ccactatggc caattggggt aattgagtta tcaacaaaaa
     2701 tagaaaagtt tatgttgccg atagcatgaa gggcaatcac acaatcatta ataacaccgt
     2761 cacaagtata aagtgcacat tgatcccatc gctgatcatc agcggcaaaa ccacccacaa
     2821 ttggcgtttt tatttcatgt tcaatgactt cttcgattaa agaagcatcg gcatgaaaat
     2881 cggagatcat gaaaattaaa ctagcttgtt gatgctcttg tagcgcagtg cgtgttgctt
     2941 gctgtggttg ctcagtaaca tgatggccaa ttttaagctg ccattgtact tgtccatgac
     3001 tatttagggc taaagctgcc gcgccatatt cacccacgcc atgagtctcg taaaaaccat
     3061 caccactatt gccaataata atgcagttag gttgattgag gctgtcttgt aatccagcaa
     3121 gaatgtcact gttatgacca taatcaatag atgtaaacaa aaaaacaact tctggattaa
     3181 gtgcagctaa gtgttcacct aaataaacac ctgctcgata ggcatctggg atttgggttg
     3241 agacagacag ggcttccatc gcattgctca tataattttt agtattgcat aacttatagg
     3301 acaaatttgt taaagctaca agagcacaag aacttgatac atctatttaa tactaaggcg
     3361 tgcgttttgt gagcttataa attaggcttt atattgtttt aattgctcac gcaccgataa
     3421 acgagcttgt cggcgaattt ctgcggcttc aaaacgttgt tgttctttct cgttttcggg
     3481 aacaagggca ggaacgggcg cgggtttccc ctcagcatca agggcaacaa aggtaaaata
     3541 ggcagaaaca atatggcgtt ttgtgcctaa ataactgttt tcggcttcaa cacgtacacc
     3601 aatttccata gaagatgtcc atgctttatt aatcgctgcg ctaaataaca aggtgtcatt
     3661 ttcttgggca ggggcaacaa aatgccaaga atccaccgaa gcagtcacac aagtatgacc
     3721 actatgacgt tctgcgacga ctactgctaa acgatcagca atgctcataa ttaagccacc
     3781 aaatacagta cgttttgcgt ttaaatcatt gagaaaaatt ttataaacat gattgtcgat
     3841 tttggaaact gaagaaactt tgctgttcat aa
//
LOCUS       DAOQMB010000217         3870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_11952_length_3870_cov_6.817038, whole genome shotgun sequence.
ACCESSION   DAOQMB010000217 DAOQMB010000000
VERSION     DAOQMB010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11952_length_3870_cov_6.817038"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3870
                     /locus_tag="PLY05_07735"
     CDS             <1..>3870
                     /locus_tag="PLY05_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03231.1"
                     /translation="RQQMDKEDDVYDFDFAILNESTSQDTQNDTTPSQTSRDNVGERD
                     KTPESSQVQRASKSEPAQANTEKRDDGVSANSGGRGDRGNLSNESGKSKTRSQESDVG
                     SARNQSSKLTDSTRSTESSDGTNLRNYRIQPNELKRTGSWRATAEQNVQIVELVKTII
                     AENRAATPEEKALLTKFTGWGASEIANGIFPNQYGQYKDANWKALGERLEAALSPEEY
                     AQAKRTTQYAHYTSEPIIRSVYAALSRFGFNGGSLIEPGMGVGLFNGLMPDAMANNSQ
                     YTGIEYDTITGNIAKLLYPDSNVIVGDFTETKLPKNFFDVAIGNPPFGSIKIQSDPEY
                     KKQGFLLHDYFFAKTIDRVKEGGLLVFVTSKGTMDKASDRARKYLSDRADLVGAIRLP
                     QTAFKDNAGTEVVTDVIFLRKRMAGQEPAGEAWAGLKEITTPQGATSINEYFATHPEM
                     ILGKSALTGSMYRANEYTVEPIAGSDIETLFSEAINNLPENIYTPQRGSKAEQAAVQR
                     RDYDPKIKKEGGIYVADDGVLMQANNGQGTPLTQKQNSKHELVDLSPKEMQWLKDYVG
                     VRDALKQAQYDQLQDNPNWQQSLETLNKTYDAFVKKHGFMLEHSVSERENEDGSTTVT
                     KRFKNKSIMGLDAESALINALEQINGDNSISKAPALLGRVINKPSTPVITNTQDAMFV
                     VFNELGRFDLAKVAKLINKSEKDVITELGDSIYEDPSSSWTTADDYLSGNVVRKLKEA
                     EQAARLNPKYQRNVTALKAVQPPALAPQDINVQLGAAWIPASDIGLFASEVLGEQRFK
                     VSYSPLTGQWSVEGTANYSSEWGIPAKDSKDILEAVLNNRQIKVTYTDANKKTHTDTE
                     KTEKANDIAKKMRESFKRWLWQDVPRTERLVTYYNEHFNNIAPRSFDGQHLTLPGVSS
                     RFDLRPHQKRAIWRTIQQGDVYYAHAVGAGKTFTMIASGMEERRLGLVKKPMYVVPNH
                     MLAQFSKEFLELYPMANIMVADEQNFHTHNRRKFVAQAALNNPDAIVITHSAFGRIGM
                     SSDYSAKFINNQIADWQEALQEVGNDERITRKQIERRIEQLENKLKALLDGAKDQLLN
                     FEELGVDRLYVDEGHEFRKLDFPTNRGNIKGIDPSGSQKSMDLYMKVEYLRSKNPNRS
                     LVMASGTPVTNTMGELFTVQRFFNPSQLEEDGDSRFDAWSAHYGEVVDGLEQNAAGGY
                     ETVSRFAKFVNVPELMSRVRYFMDILTSNQLGDLVQRPDVIGGGRQVIVTPAPDGYKE
                     YQKDLEKRIKAIRDRKAPPKPGDDIIL"
BASE COUNT         1208 a          791 c          921 g          950 t
ORIGIN      
        1 tccgtcaaca aatggataaa gaggatgatg tatatgattt tgattttgct atactgaatg
       61 aaagcacctc acaggacaca caaaatgaca ccacaccaag ccaaacaagt cgagacaatg
      121 ttggagagcg agataaaacg cctgaatcca gccaagtaca acgagctagt aagtcagaac
      181 ctgctcaagc caacactgaa aaacgtgatg acggagtatc tgcaaacagt ggaggacgag
      241 gagacagagg caatctttca aatgaatcag gaaagagcaa aacacgaagc caagaatccg
      301 atgtgggcag tgcaagaaat caatcgtcta aactcacaga ttcaacacgc agcactgaat
      361 caagcgatgg aacgaattta cgcaattacc gaatccagcc caatgagtta aagcgtacag
      421 gcagttggag ggcaaccgca gagcaaaacg tacagattgt tgagcttgtc aaaaccatta
      481 ttgccgaaaa tcgtgccgcg acacccgaag aaaaagcctt attgactaag tttacaggtt
      541 ggggcgcgtc tgaaattgct aacggcatat tccccaatca gtacggccaa tacaaagatg
      601 ctaactggaa agcattaggg gagcgattag aagccgccct aagccccgaa gaatacgccc
      661 aagccaaacg cactacacaa tacgcgcatt acaccagtga gccaattatt cgcagtgtgt
      721 atgctgcatt gtcacgtttt ggttttaatg gcggttcact gattgagcct ggtatgggcg
      781 ttggtttatt taatggtttg atgcctgatg ctatggctaa taacagtcaa tacacaggca
      841 ttgaatacga caccatcacg ggcaacattg ccaaactgct ttaccccgat agtaatgtca
      901 ttgtgggtga ctttaccgaa actaagctgc ctaaaaactt ttttgatgta gcaattggta
      961 atccgccttt tggtagcatc aagattcaat ctgaccctga atataaaaaa caaggctttt
     1021 tgctgcacga ttactttttt gccaaaacca ttgaccgtgt caaagagggt ggtttgttgg
     1081 tgtttgtgac atcaaaaggc acaatggata aagccagtga ccgtgcgcgt aagtatttat
     1141 cagaccgtgc cgatttagtg ggggcaatcc gtttgccaca aacagcgttt aaggataatg
     1201 caggtacgga agtggtgact gatgttatct ttttacgcaa gcgcatggca gggcaagagc
     1261 cagcagggga ggcatgggca gggttaaaag aaatcaccac gccacaaggc gcaacatcaa
     1321 tcaatgagta ttttgccact caccctgaaa tgattttagg caagagtgcc ttaacaggtt
     1381 caatgtaccg tgccaatgaa tacacggttg aaccgattgc agggagcgat attgagacct
     1441 tgtttagcga ggcaatcaat aacctacccg aaaacattta cacgccacag cgaggcagta
     1501 aggccgaaca agcagccgta caacgtagag actatgaccc taaaattaaa aaagagggcg
     1561 gtatttatgt agccgatgat ggtgtgttga tgcaggcaaa caatggtcaa ggcacaccac
     1621 tcacccaaaa acaaaacagc aaacatgaat tagtggattt aagtcctaaa gagatgcaat
     1681 ggctcaagga ttatgtgggg gtgcgtgatg cgcttaaaca ggcgcaatat gaccaactgc
     1741 aagataaccc caactggcaa cagtctttag agacacttaa caaaacctat gatgcctttg
     1801 ttaaaaagca tggttttatg ctagagcata gcgtgtctga gcgtgaaaac gaagatggct
     1861 caaccactgt gacaaagcga tttaagaata agtctatcat gggcttagat gcagaaagcg
     1921 cgttgataaa tgctttagag caaataaatg gcgataactc catctctaaa gctcctgctt
     1981 tattaggtcg cgtaattaac aagccctcaa cgcctgtcat tactaacacc caagatgcaa
     2041 tgtttgtggt gtttaatgag ttggggcgtt ttgacttagc caaagtcgcc aagttaatca
     2101 acaagtccga aaaagacgtg attaccgaat tgggtgattc gatttatgaa gacccatcta
     2161 gtagctggac aacagccgat gattatttgt cgggcaatgt ggttcgcaaa ctcaaagaag
     2221 ctgaacaagc ggcgcgattg aaccccaaat atcagcgtaa cgtaacagcc ttaaaagcgg
     2281 tgcaaccgcc agcccttgca ccacaagata ttaacgtaca gcttggcgcg gcatggattc
     2341 ctgccagtga tattggcttg tttgcgtctg aagtattggg tgagcaacgc tttaaggtaa
     2401 gctattcgcc attaacaggt caatggtcag tggaaggtac tgctaattat tcgtctgagt
     2461 ggggtattcc tgctaaagat agtaaggata ttttagaggc ggtgttaaac aaccgtcaaa
     2521 ttaaagttac ttacactgat gccaacaaaa aaacacatac cgacaccgaa aaaaccgaaa
     2581 aagccaacga tattgccaaa aagatgcgtg aatcgtttaa gcggtggttg tggcaagatg
     2641 ttccgcgtac tgagcgatta gtgacttatt acaatgagca ttttaataac attgctccac
     2701 ggagttttga tggtcagcac ttaacattac ctggtgtgtc ttctcgtttt gatttgcgcc
     2761 ctcatcaaaa acgcgctatt tggcgaacta ttcagcaagg tgatgtttac tatgctcatg
     2821 cggtcggggc aggcaagacc tttactatga ttgcttcggg catggaagaa cgcagattag
     2881 gcttggttaa aaaaccgatg tatgttgtac ctaatcatat gttggcacaa tttagtaaag
     2941 agtttttaga gctttaccca atggccaata tcatggtggc tgatgagcaa aactttcaca
     3001 cgcacaaccg ccgcaagttt gtagcacaag cggcgttaaa caatcctgat gcgattgtga
     3061 ttactcattc tgccttcggg cgtattggca tgagtagtga ctactcggcc aagtttatca
     3121 ataatcaaat tgcagactgg caagaagcat tgcaagaagt tggcaatgat gaacgcatta
     3181 cacgaaaaca aatagaaaga cgtatagagc agcttgaaaa caaactcaaa gccttacttg
     3241 atggcgcaaa agaccaattg cttaattttg aggagttggg cgttgacagg ctttatgtag
     3301 atgaagggca tgagtttaga aaattagact tccccaccaa tcgaggcaac attaaaggta
     3361 ttgacccgtc aggctcgcaa aagtcgatgg acttgtacat gaaagttgaa tacttgcgta
     3421 gtaaaaatcc caatcggtca ttggtgatgg cttcgggaac gcctgtgact aacactatgg
     3481 gcgagttgtt taccgtgcaa cgattcttta acccatcaca attagaagaa gatggcgata
     3541 gtcgttttga tgcgtggtcg gcgcattatg gtgaagtggt ggacggatta gaacaaaatg
     3601 ccgcaggagg ttatgagact gtcagtcgct ttgctaagtt tgtgaatgtg cctgagttaa
     3661 tgagccgtgt tcgctacttt atggatattt tgaccagtaa tcagttgggc gacttggtgc
     3721 aacgtcctga tgttatcggt ggtggtcgtc aagtaattgt tacgcctgcc cctgatggct
     3781 acaaagagta tcaaaaagac ctagagaagc gcattaaagc cattcgtgac agaaaagctc
     3841 cacccaaacc aggtgacgat attattttaa
//
LOCUS       DAOQMB010000218         3848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12068_length_3848_cov_15.235434, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000218 DAOQMB010000000
VERSION     DAOQMB010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12068_length_3848_cov_15.235434"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="rluB"
                     /locus_tag="PLY05_07740"
     CDS             complement(<1..869)
                     /gene="rluB"
                     /locus_tag="PLY05_07740"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HNC03232.1"
                     /translation="MSEKLQKVLARVGLGSRRHMEEAIVEGRVSVNGKIAEIGQRIEA
                     TDELRIDGRRVSFTLEEETRRRVLVYYKPEGEICSRQDPEGRPTVFDNLPPLNGERWV
                     MVGRLDINSSGLLLFTNDGELANRLMHPSNEVEREYAVRVMGEITPEMRQNLLNGVML
                     EDGPAKFESMSEVGGEGINRWYEVVVKEGRNREVRRLFESQGLKVSRLLRIRYGTVSL
                     ARHLRIGRWLELDKDEIDGLAALVKLKPRIGTGLYGLAKRRAEKLEENPLPARRGGYL
                     RQQKRDVAEKRGKS"
     gene            complement(994..1575)
                     /gene="scpB"
                     /locus_tag="PLY05_07745"
     CDS             complement(994..1575)
                     /gene="scpB"
                     /locus_tag="PLY05_07745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNC03233.1"
                     /translation="MKLSQSLLKNILESAIFASPSPLSVEELLSLFADEEKPTRQQIQ
                     TLIKELQNDYATRPLEVVEVASGYRFQVRAKFSPWISKMWQDKPPKLSRAMLETLAII
                     AYRQPVTRAEIEAIRGVVVSTQIIKALFDRDWIVVVGHREVPGRPALLATTREFLDAF
                     SLKGLADLPPLAALRDLDTIEAELATTNRVEKE"
     gene            complement(1572..2405)
                     /locus_tag="PLY05_07750"
     CDS             complement(1572..2405)
                     /locus_tag="PLY05_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNC03234.1"
                     /translation="MTELVINTPEVLMLDNQQQALARVYDEFITQVPDDLYIPPDALA
                     VFLEAFEGPLDLLLYLIRKQNLDILDIPIAEITRQYISYIELMQELNIDLAAEYLLMA
                     ALLAEIKSRLLLPKPKNPEAEEDDPRAELIRRLQEYERFKKAAEDLGDLPQEGRDIFL
                     THVEPPVRELLATEPFVGDFSLLLEAMRDVLQRAQLQKSHIIAEEGLSLEQRFREVLA
                     RLASGETVIFYELFTVKEGRIGVVVTFMAILELLKQKMIELVQESVFAPIYVREYREL
                     A"
     gene            complement(2413..3624)
                     /locus_tag="PLY05_07755"
     CDS             complement(2413..3624)
                     /locus_tag="PLY05_07755"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNC03235.1"
                     /translation="MSVAASQRVLSGMRPTGQLHLGHYHGVLKNWAKLQYEHDCFFFV
                     ADWHALTTAYEDPRIIKQSVFAMVVDWLAAGVNPKTATIFVQSQMPQHAELHLLLSMM
                     TPLSWLERVPTYKDQQEKLKEKDLATYGFLGYPLLQSADILLYRAGLVPVGADQVAHI
                     ELTREVARRFNHLFGKEIGFEDLVAAAIQKMGKKASKVYVDLRKKYQESGDVEALATA
                     QALVQAQQNITLGDKERLLGSLEGGGKIILPEPQPLLTPAAKMPGLDGQKMSKSYGNT
                     ISLREAPDDVDQKVRRMPTDPARIRRTDAGNPDVCPVWQLHEIYSDESTKQWVQQGCR
                     SAGIGCLECKKPITEAINAELEPMRVRAREYEESPELIYSLLTEGAERARDVAEDTLK
                     DVRSAMGLNYR"
     gene            complement(3667..>3848)
                     /locus_tag="PLY05_07760"
     CDS             complement(3667..>3848)
                     /locus_tag="PLY05_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03236.1"
                     /translation="YLYKKTEKTIAKMKYTSNINPQPVFGMRLVLLPFLGIILHVYGS
                     FCFLGRPSGLPFFFV"
BASE COUNT         1145 a          930 c          675 g         1098 t
ORIGIN      
        1 gatttgcctc gcttctcggc aacatcgcgt ttttgctgac gcaaatagcc accacgacgc
       61 gctggtaaag gattttcctc taatttttca gcacgacgtt tagccaaacc atacaagcct
      121 gtaccaatac gcggttttag cttaactaaa gccgccaagc catcaatttc gtctttatct
      181 aactctaacc aacgtccaat acgcaaatga cgcgctaaag acactgtgcc ataacgaatc
      241 cgtaataaac ggctaacttt taaaccttgt gattcaaata aacgccgtac ttcgcggttg
      301 cggccttctt tgaccaccac ttcataccag cggttaatgc cttcaccacc cacttcgctc
      361 attgactcaa acttggcggg gccatcttcg agcatcacgc catttaataa gttttgacgc
      421 atttctgggg taatttcgcc catcacacgc accgcatatt cgcgctcaac ttcattagac
      481 ggatgcatta aacgatttgc caattcacca tcgtttgtaa ataacaataa accactactg
      541 ttaatatcca aacgcccaac catgacccaa cgctcaccat ttagcggagg taagttgtca
      601 aatacggttg gccgaccttc agggtcttgg cgagaacaaa tttcgccttc aggcttgtaa
      661 tacactaaca cacggcgacg tgtttcttct tcaagggtaa aagacacacg acggccatca
      721 attcttaatt cgtctgttgc ttcaatgcgc tgaccaattt cagcaatttt accattgacg
      781 ctaacacggc cttcaacaat cgcttcctcc atgtgacgac gagaacctaa accaacacgg
      841 gctaggactt tttgcaattt ttcgctcatg gctcttcaat actcactatt taaccaacag
      901 tattaatctt tcagacaaga gttttcactt tgtctacttt ggattggtgc tatagcacta
      961 atccttctct accctacagt cacaatgtgg ctattattct ttttccacac gatttgttgt
     1021 agccaattcg gcttctatag tatctaaatc acgcaaagct gctaagggcg gcaaatcagc
     1081 taagcctttc aaactaaacg catccaaaaa ctcacgagtc gttgccaata aagcgggtct
     1141 acctggcact tcgcgatgac caacaaccac aatccaatct cggtcaaaaa gggctttaat
     1201 gatttgcgta ctcaccacga caccacgaat tgcttcaatt tcagctcggg taacaggttg
     1261 gcgataagca ataatcgcta aagtttctaa catcgcccgt gagagcttgg gtggcttgtc
     1321 ttgccacatt ttgctaatcc acggactaaa cttcgcacgg acttgaaaac gataaccaga
     1381 ggcaacctca accacttcta aagggcgcgt tgcatagtca ttttgcaact ctttaatcag
     1441 cgtttgtatt tgttgacgag tcggtttttc ctcatcagca aataaactta ataactcttc
     1501 aaccgacaaa gggctaggac tggcaaatat cgcactttcg aggatatttt tcagtaagct
     1561 ctgactgagc ttcatgctaa ctccctatat tctctcacat aaatcggcgc aaaaacgctt
     1621 tcttgtacta attctatcat tttttgtttc aataactcta aaatagccat aaaagtcaca
     1681 acaacaccga ttcgcccttc tttgacggtg aataactcat aaaatataac cgtttcacca
     1741 gacgccaaac gcgctaaaac ttctctgaaa cgttgttcta aacttaagcc ttcttctgca
     1801 atgatatgac ttttttgcaa ctgcgcacgt tgtaatacat cacgcattgc ttctaataac
     1861 aaagaaaaat cgcccacaaa aggctcagtc gccaataact ctcgaactgg cggttcgaca
     1921 tgggttaaaa aaatgtctct accttcttgt ggtaaatcac ccaaatcttc ggctgccttt
     1981 ttaaaacgct catactcttg taaacgacga attaactcgg cgcgtgggtc atcctcttcg
     2041 gcttcaggat tttttggctt aggcaacaat aaacgcgatt taatttcggc caacaaagcc
     2101 gccattaata aatactctgc tgctaaatca atatttagct cttgcattaa ttcaatataa
     2161 ctaatgtatt ggcgggtaat ttcggcaatg ggaatatcta atatatccaa gttttgcttg
     2221 cgaattaaat acagcaataa atctaaaggc ccttcaaatg cttctaaaaa tactgccaag
     2281 gcatcaggcg gaatatataa atcatcaggc acttgggtaa taaactcatc ataaacacgc
     2341 gccaatgcct gttgctgatt atccaacatc aaaacttcgg gcgtatttat cacaagttcg
     2401 gtcattttgt gtttaccgat aatttaaacc catcgcacta cgcacatctt tcagtgtgtc
     2461 ttcagcaaca tcacgtgcac gttcagcacc ttcagttaat aaactataga ttaactctgg
     2521 gctttcttcg tattcacgcg cacggacacg cattggctct aattcggcat taatcgcttc
     2581 ggtaattggc tttttacact ctaaacaacc aataccagct gagcgacaac cttgttgaac
     2641 ccattgtttg gttgattcat ccgaatagat ttcatgtaat tgccacactg gacacacatc
     2701 aggattaccc gcatcggtac gacgaatacg agcaggatca gttggcatac gacgcacttt
     2761 ttgatcaacg tcatcaggtg cttcacgcaa agaaatggta ttaccataag atttagacat
     2821 cttttggccg tctaaaccag gcattttagc cgcaggtgtt aacaaaggct gtggttctgg
     2881 cagaataatt ttgccaccac cttctaaaga gcctaataaa cgctctttgt cacccaacgt
     2941 aatattttgt tgagcttgca ctaaggcttg tgcagtggct aaagcttcaa catcacccga
     3001 ctcttgatat tttttgcgta aatcaacata cactttgcta gcttttttgc ccattttttg
     3061 aatggcggct gcaactaagt cttcaaagcc aatttcttta ccaaataaat gattaaaacg
     3121 acgagccact tcgcgagtta attcgatatg tgcgacttgg tctgcaccga caggcactaa
     3181 acctgcacga tataataaaa tatcggcact ttgtaataag ggatagccta aaaagccata
     3241 ggtggctagg tctttctctt ttaacttttc ttgttgatct ttataggttg gcacacgctc
     3301 taaccatgat aaaggcgtca tcatcgacaa caataaatgc aactcagcat gttgtggcat
     3361 ttgtgattgg acaaaaatag ttgctgtttt agggtttacg ccagcggcca accaatcaac
     3421 caccatcgca aaaacacttt gtttaataat gcgaggatct tcataggccg ttgttaaagc
     3481 gtgccaatct gcaacaaaaa agaaacagtc atgctcatat tgtaattttg cccagttttt
     3541 tagcacacca tgataatggc ctaaatgtaa ctgacctgtt ggacgcatac ctgataaaac
     3601 acgttgagaa gcggcgacac tcaccctaaa cactccaaaa gagaaaaaga tttttaaatt
     3661 atacgattag acaaagaaaa aaggcagacc cgaaggtctg cccaaaaaac aaaaactacc
     3721 ataaacatgg aggatgatac ctaagaaagg caacaaaacc aacctcatcc caaagactgg
     3781 ctgtggattg atattactcg tatatttcat ttttgcaatg gttttttcgg tttttttata
     3841 taaataaa
//
LOCUS       DAOQMB010000219         3824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12193_length_3824_cov_13.706819, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000219 DAOQMB010000000
VERSION     DAOQMB010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12193_length_3824_cov_13.706819"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PLY05_07765"
     CDS             <1..263
                     /locus_tag="PLY05_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03237.1"
                     /translation="CLKQENCSTWALLGAWQGTAEGLHLQALAEDTGFLADLNAEAEL
                     SDIVNQLHIQRLKKALHHSPDLMTTIKLKKELAAATAALAKK"
     gene            362..2257
                     /gene="rpoD"
                     /locus_tag="PLY05_07770"
     CDS             362..2257
                     /gene="rpoD"
                     /locus_tag="PLY05_07770"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNC03238.1"
                     /translation="MTTENVQNESEAQTTSQLAALITRGKEQGYLTYAEVNDHLPESI
                     TETEQIEDIIQMLTDLGIPVHERAPEADDLTLDETPDASDDDVAAADAIAVLTSVENE
                     PGRTTDPVRMYMREMGTVELLTREGEIAIAKRIEEGVRDVLHSMACWPTVVPSVLADY
                     DRVGTGERRLAEILVGYLDLEDTSIPEVIEEAELDLDAEAEAPVKAADIDLDSEESEG
                     SDDEEEGESGPDPEIARQRFAELTEQYQKTLAVLKNHDRYSAEGKAAINDLANVFMLF
                     KLTPRLFEFFGQELRAGQDAVRAQEKAIMVLCIRRAKMDRKTFISEFPGHEINLSWVD
                     SQIATNKSWSTGLKQFRDEIIKAQQKLIDLEQELGLRVAEIKDINRRMSIGEAKARRA
                     KKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATW
                     WIRQAITRSIADQARTIRIPVHMIETINKINRVSRQMLQEMGREPSPEELGERLDMPE
                     DKVRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTMTSPVDAATAEGLREATREVLA
                     NLTPREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEHL
                     RSFLDNE"
     gene            2416..3333
                     /locus_tag="PLY05_07775"
     CDS             2416..3333
                     /locus_tag="PLY05_07775"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC03239.1"
                     /translation="MNPFNLQDITTFLAIVDSGSLTQAAERLEVSKSIVSKRLKRLEE
                     MLGVALLHRSTRGITPTEQGQAFHLRASNILQELEATVDEMTEGNQNLCGSLRIAAPV
                     TFGTMYLGRLVFPFISKHPRLHVAIHLDDKRVDIVQEQFDLAIRISAEPGLSLKARQL
                     GLCKLIVVCSPEYARQYNLKSLNEDLFAHEYIGYSNTPDSQLRAILTEQNQHSQLTSR
                     FTANSGEMNRDAAIAGLGLAVLPLFMVAEALRTGQLINAMPDLELEPIPIYAVYPPTR
                     HVPKKIRMLIDHLCQELGDNPPWEQDLPL"
     gene            complement(3390..3710)
                     /locus_tag="PLY05_07780"
     CDS             complement(3390..3710)
                     /locus_tag="PLY05_07780"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HNC03240.1"
                     /translation="MFPIKLNQEWTGLMEQYQQDHQDPRNQFCHKIGIPLIAGSLPLG
                     ATIIGLPLAVPMFTVGWGFQFVGHYFEGKKPSFVEDRRQLVVGVLWWTKKVGLKLVKT
                     VDEA"
BASE COUNT         1176 a          805 c          799 g         1044 t
ORIGIN      
        1 tttgtcttaa acaagaaaat tgtagtacat gggcattatt aggcgcgtgg caaggtacag
       61 ccgaaggttt acatttacaa gctttagccg aagacacagg atttttagcc gatttaaatg
      121 ctgaagccga gttaagcgat attgtgaatc aactacatat tcaacgacta aaaaaagctt
      181 tacatcacag tcccgattta atgaccacca ttaaattaaa aaaagaactg gctgccgcaa
      241 ctgctgctct tgcaaaaaaa taaatgaatc cctatttagc gactataatc taaagaagct
      301 taaaccttat tgacagacca tgctatacta gtagtcttgt ctgcatcaca gagaaaatcg
      361 tatgactacc gagaacgtgc aaaacgaaag tgaagcccaa accacctcac aacttgcagc
      421 cctgattact cgtggtaaag agcaaggtta tttaacctat gccgaggtca acgaccattt
      481 acccgaatct atcactgaaa cggagcaaat tgaagatatt attcaaatgc tcaccgattt
      541 gggtattcca gttcacgagc gcgcccctga agccgatgat ttaacgcttg atgaaacacc
      601 tgatgccagt gatgacgatg tcgcggctgc cgatgcgatt gccgttttaa cctcggtaga
      661 aaacgaacct ggtcgcacca ctgacccagt gcgtatgtat atgcgtgaaa tgggtacagt
      721 tgagctgtta acccgcgaag gcgaaattgc cattgccaaa cgtatcgaag aaggtgtgcg
      781 tgatgtatta cattcaatgg cttgttggcc aaccgttgta ccgagtgtct tagccgatta
      841 tgaccgtgtc ggtacgggcg aacgccgttt ggccgaaatt ttagtcggtt atttagacct
      901 cgaagacacc tctattccag aggtgattga agaagcggaa ctcgatttag acgcagaagc
      961 cgaagcacct gttaaagcgg ccgatattga tttagatagc gaagaaagtg aaggtagtga
     1021 cgatgaggaa gaaggcgaat ctggcccaga ccccgaaatt gcacgacaac gttttgctga
     1081 attaaccgaa caatatcaaa aaacgctagc ggtattaaaa aaccacgatc gttatagtgc
     1141 cgaaggcaaa gcagcgatta atgatttagc caatgtgttt atgttgttta aattgacacc
     1201 tcgtttattt gaattttttg gtcaagaatt acgtgctggt caagatgcag ttcgcgccca
     1261 agaaaaagcg attatggtat tgtgtattcg ccgtgccaaa atggatcgca aaacctttat
     1321 tagtgaattt cccggtcacg aaattaattt aagttgggtt gatagccaaa ttgccaccaa
     1381 taaatcatgg tctactggtt taaaacaatt ccgtgatgaa atcatcaaag cccaacaaaa
     1441 attaattgat ttagaacaag aattgggttt gcgtgttgct gaaatcaaag acatcaaccg
     1501 tcgtatgtcg attggtgaag ccaaagcccg tcgtgccaaa aaagaaatgg ttgaagccaa
     1561 cttacgtttg gttatctcga ttgccaaaaa gtacaccaac cgtggtttac aattcttgga
     1621 cttaatccaa gaaggtaaca ttggcttgat gaaagcggtt gataaatttg aatatcgtcg
     1681 tggttataaa ttctcaactt atgcaacatg gtggattcgt caggcgatta cccgttcgat
     1741 tgctgaccaa gcgcgtacga ttcgtattcc tgtacacatg attgaaacca ttaacaaaat
     1801 taaccgtgtt tctcgtcaaa tgttacaaga aatgggacgt gaaccctcac ccgaagaatt
     1861 gggcgagcgt ttagatatgc ctgaagacaa agtgcgtaag gtgttaaaaa ttgctaaaga
     1921 gcctatctcc atggaaacac cgattggtga tgatgaagat tcgcatttag gtgactttat
     1981 tgaagacacc accatgacgt cacctgttga tgcagcaacg gctgaaggtt tacgtgaagc
     2041 cactcgtgaa gtgttggcca acttaacacc acgcgaagca aaagtgttaa gaatgcgttt
     2101 tggtattgat atgaataccg accacacctt agaagaagtg ggtaaacaat ttgatgtaac
     2161 acgcgaacgt attcgtcaaa ttgaagccaa agcgttgcgt aaattacgtc acccttctcg
     2221 ctccgaacat ttacgttcgt tcttggataa tgagtagctt cgactacgct cggctaccac
     2281 gactctgctc tttgagcgga gtcgaagggt gaggtctttt aaaaagactt ttaccaaaag
     2341 cctctttacg ttagtctagc gtaaagaggc tttttagttt tcagcgatat taaaaccact
     2401 agaacaacaa acatcatgaa tccatttaat ctgcaagata tcaccacttt tttggccatt
     2461 gttgatagtg gtagtttaac ccaagccgcc gaacgccttg aggtatccaa atcgattgtc
     2521 agtaagcgcc ttaaacgttt agaagaaatg ttaggtgtcg ccttattaca tcgctcaaca
     2581 cgcggcatta cccctaccga acaaggccaa gcttttcatt tgcgcgcctc taatattttg
     2641 caagaacttg aagccaccgt tgatgaaatg accgaaggta atcaaaactt atgtggctcg
     2701 ttgcgtattg ctgcacctgt gacctttggt accatgtatt tagggcgatt agtatttcct
     2761 tttattagta aacacccacg cctacatgtg gccattcatt tagatgataa acgtgttgat
     2821 attgttcaag aacagtttga ccttgcaatt cgtattagtg ccgaaccggg tttgtcactc
     2881 aaagcccgac aattaggctt gtgtaaacta atcgttgttt gtagtccaga atatgctcga
     2941 caatataatt taaaaagttt aaatgaggat ttatttgccc atgaatatat tggctacagc
     3001 aacactccag acagtcaatt aagagctatt ttaaccgaac agaatcaaca tagccaactt
     3061 acaagtcgat ttacagcgaa tagtggtgaa atgaatcgag atgcagccat tgcaggatta
     3121 ggcttggcag ttttaccatt atttatggtg gctgaagcct taagaacagg tcaattaatt
     3181 aatgccatgc ctgatttaga attagagcct ataccgattt atgcggttta tccaccaaca
     3241 cgtcatgtac ccaaaaaaat cagaatgttg attgaccatt tatgtcaaga attgggagat
     3301 aatccaccgt gggaacagga tttaccgttg taaaaagaca gatattgccc tcaccccaac
     3361 cctctcccac aggagaggcc ttcttctatt taagcttcat ccacggtttt aactaatttt
     3421 aaaccgactt tttttgtcca ccacaaaaca cccaccacta actgacggcg atcttcaaca
     3481 aaagatggtt ttttgccttc aaaataatga ccaacaaatt gaaagcccca accaacagta
     3541 aacataggta cagccaatgg caagccaata atggttgccc ctaaaggtaa agagcctgca
     3601 attaaaggaa taccgatttt gtgacaaaat tgattacgtg ggtcttgatg gtcttgttga
     3661 tattgttcca tcaagcctgt ccactcttgg tttaatttaa ttggaaacat gtgcttaccc
     3721 tctaatagcc ttgtatatgg gcagaatttg acatcctccc cgccctcaaa gggcgaggat
     3781 tccttctaca agacggcaat gtcctgccgc acacctcact tagt
//
LOCUS       DAOQMB010000220         3785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12406_length_3785_cov_15.746381, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000220 DAOQMB010000000
VERSION     DAOQMB010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12406_length_3785_cov_15.746381"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2579)
                     /gene="acnD"
                     /locus_tag="PLY05_07785"
     CDS             complement(<1..2579)
                     /gene="acnD"
                     /locus_tag="PLY05_07785"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HNC03241.1"
                     /translation="MNSQFRKKLPNTNLDYFDARAAVDAIKAGAWATLPYTARIHAEN
                     IVRKADPAIINDCLTQLIERKRERDFPWFPARVVCHDILGQTALVDLAGLRDAIADQG
                     GDPAKVNPVVPVQLIVDHSLAVECGGFDPDAFQKNRDIEERRNEDRFHFINWTKTAFD
                     NVDVIPAGNGIMHQINLEKMSPVIHSDNGVAYPDTCVGTDSHTPHVDALGVISVGVGG
                     LEAENVMLGRASWMRTPDMVGVELTGTRQAGITATDMVLALTEFLRKEKVVGAYLEFY
                     GEGVPCLTIGDRATISNMAPEYGATAAMFSIDNQTLDYLRLTGRSDAQVALVETYAKE
                     AGLWSDSLVNAQFERVLKFDLSTVVRNMAGPSNPHRRLPVSALKERGIAVDLDKARAE
                     EAQGLMPDGAVIIAAITSCTNTSNPRNVIAAGLLARNARQLGLVRKPWVKSSLAPGSK
                     VVELYLKEAGLLEDLEALGFGIVAFACTTCNGMSGALDPKIQQEIIDRDLYATAVLSG
                     NRNFDGRIHPYAKQAFLASPPLVVAYAIAGTVRFDIEQDVLTVVDGKEIRLKDIWPSD
                     EEIDAIVAKAVKPEQFNQVYIPMFEKQDAEKAASPLYDWRPQSTYIRRPPYWEGALAG
                     ERTLKGMRPLALLPDNITTDHLSPSNAILMNSAAGEYLHKMGLPEEDFNSYATHRGDH
                     LTTQRATFANKELVNEMAIVDGQVKKGSLARVEPEGQVMRMWEAIETYMNRKQPLIII
                     AGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFKAGTTRKTL
                     GLDGTETYDVVGKLTPRADLTLVVNRKNGETVEVAVTCRLDSDEEVSVYEAGGVLQRF
                     AKD"
     assembly_gap    2580..2679
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2680..>3785)
                     /gene="prpC"
                     /locus_tag="PLY05_07790"
     CDS             complement(<2680..>3785)
                     /gene="prpC"
                     /locus_tag="PLY05_07790"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF009006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HNC03242.1"
                     /translation="KKSVALSGTAAGNTALCTVGRSGNDLHYRGYDILDIATTSEFEE
                     VAYLLVHGKFPNTAELAGYKAKLKALRGLPAAVKVALEQLPPSSHPMDVMRTGVSVLG
                     CVKPEKEDHNHPGARDIADKLMACLGSMLLYWYHFAYNGKRIDVETDDDSIGGHFLHL
                     LHGQKPSESWVRAMHTSLILYAEHEFNASTFTGRVVAGTGSDMYSAICGAIGALRGPK
                     HGGANEVAFEIQKRYDTPDEAEADIRERVGRKEVIIGFGHPVYTVSDPRNVVIKKVAH
                     ELSVEQSNTKMYDIAERLESVMWEIKKMFPNLDWFSAVSYHMMGVPTDMFTPLFVIAR
                     TSGWSAHIIEQRIDGKIIRPSANYTGPEDQKFVP"
BASE COUNT         1044 a          917 c          740 g          984 t
ORIGIN      
        1 aagtccttag caaaacgttg caacacacca cctgcttcat aaacagacac ttcttcgtca
       61 gaatccaaac gacacgtcac tgctacttca acagtttcac catttttacg attaaccact
      121 aaggttaaat cggcgcgtgg tgttaattta cctacaacat cataagtttc tgtgccatcc
      181 aagcccaaag tcttacgcgt tgtgccagct ttaaactcta aaggcaatac acccatacca
      241 atcaaattag tacggtgaat acgctcaaag ccttcagcca caatcgcttc aacacctgct
      301 aaacgcacgc ctttagccgc ccagtcacgg ctagaaccct gaccataatc cgcacccgca
      361 ataataatca acggttgttt gcggttcatg taggtttcaa tggcttccca cattctcatc
      421 acttggcctt ctggctcaac acgtgccaac gagccttttt tcacctgacc atccacaatc
      481 gccatttcgt taaccaattc tttgttagca aaagtcgcac gttgagtggt caaatggtca
      541 ccacggtggg tggcgtagct gttaaagtct tcttcaggca agcccatttt atgcaagtat
      601 tcacctgcgg cactgttcat caaaatcgca ttcgatggcg ataaatggtc agtggtgatg
      661 ttgtctggca acaaggctaa tggacgcatc cctttgagcg tgcgctcccc tgccaatgca
      721 ccttcccaat acggtggacg gcgaatataa gtgctttgtg gtcgccagtc atacaacgga
      781 gaagcagctt tttcggcatc ttgcttctca aacatgggga tataaacttg attaaattgt
      841 tctggcttaa ccgccttggc gacaatcgca tcaatttctt cgtcacttgg ccaaatatct
      901 tttaaacgaa tttctttgcc atcgaccacc gttaacacat cttgttcaat atcaaaacgt
      961 actgtacctg caatcgcata agccacgact aagggtggag atgccaagaa agcttgttta
     1021 gcatagggat gaatacgacc atcaaagtta cggttacccg ataatacagc cgtagcgtac
     1081 aaatcacggt caatgatttc ttgttgaatt ttcgggtcta atgcaccact cataccatta
     1141 cacgttgtac aagcaaacgc aacaatacca aaaccaagtg cttctaggtc ttctaataaa
     1201 cctgcttctt ttaagtacaa ttcaacaact ttagagccgg gtgctaaaga ggatttaacc
     1261 caaggcttac gcaccaaacc taattgacga gcattacgtg ctaacaaacc agccgcaatc
     1321 acgttgcgtg ggttggaagt gttagtacaa ctggtgattg ctgcaataat cactgcccca
     1381 tcaggcatta aaccttgtgc ttcttcggca cgcgctttgt ctaaatcaac ggcaatacca
     1441 cgctctttta aggcagaaac aggcaaacgg cgatgtgggt tagatgggcc agccatgttt
     1501 ctcacgactg ttgacaagtc aaatttaagc acacgctcaa actgtgcatt gaccaaactg
     1561 tcagaccaca aacctgcttc tttggcatag gtttcaacta aggcaacttg agcatcagaa
     1621 cgacccgtca aacgcaaata atctaaggtt tggttgtcga tagagaacat tgctgccgtt
     1681 gcgccatatt caggtgccat gttggagata gttgcgcggt caccaatggt taaacacggt
     1741 acaccttcac catagaactc taaataagca ccaaccactt tttctttacg caaaaattcg
     1801 gttaaagcta agaccatatc cgttgcggtg atgcctgctt gacgtgtgcc agttaattca
     1861 acaccaacca tgtctggtgt acgcatccat gaagcgcgac ccaacatcac gttttcggct
     1921 tctaaaccac cgacacccac cgaaataacc cctaaagcat caacgtgtgg tgtgtgtgaa
     1981 tctgtaccga cacaggtatc aggataggcc acgccattgt cactgtgaat cacaggtgac
     2041 attttttcga ggttgatttg gtgcataatg ccgttacccg ctgggataac atcgacatta
     2101 tcaaaggctg tttttgtcca gttaataaag tggaaacggt cttcgttgcg gcgttcttcg
     2161 atgtcgcggt ttttttgaaa agcatcaggg tcaaaaccac cacactctac ggctaaagaa
     2221 tggtcaacga ttaattgcac tggcacaaca ggattaactt tggctgggtc gccaccttgg
     2281 tcggcaatcg catcacgtaa gcctgctaag tcaactaaag cggtttgacc taaaatatca
     2341 tgacaaacca cacgcgcagg aaaccacgga aaatcgcgtt cacgtttacg ctcaattaat
     2401 tgtgttaaac agtcattgat aatggctgga tcggctttgc ggacaatgtt ttcggcatga
     2461 atacgtgcgg tatagggcaa tgttgcccaa gcacctgcct tgatagcatc aaccgcagca
     2521 cgtgcgtcaa aatagtctag gttggtattg gggagttttt tgcggaattg gctgttcatn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna atggcacaaa tttttgatct
     2701 tctgggccag tgtagttagc acttggacga ataatcttgc catcaatacg ttgttcaata
     2761 atgtgtgcag accaaccaga ggtacgggca atcacaaaca acggtgtaaa catatccgtt
     2821 ggcacaccca tcatgtgata gctcaccgcc gagaaccaat ctaagttcgg gaacatcttt
     2881 ttgatttccc acatcacact ttctaaacgt tcagcaatgt catacatctt ggtattgctt
     2941 tgctcaacac ttaattcatg agcaaccttt ttaatcacca cgttacgtgg gtcgctaacc
     3001 gtataaacag gatgaccaaa accaataatc acttctttgc ggcctacacg ctcacgaata
     3061 tctgcttcgg cttcgtctgg tgtgtcgtaa cgcttttgga tttcaaaagc cacttcgtta
     3121 gcaccaccat gtttagggcc acgcaatgca ccaatcgcac cacaaatcgc tgaatacata
     3181 tcagagcctg taccagcaac cacacgacct gtaaacgtag aggcattaaa ctcatgctct
     3241 gcgtataaaa ttaaggaggt gtgcatagca cgaacccagc tttcgcttgg cttttgacca
     3301 tgtaacaaat gtaagaaatg accaccaatg gagtcgtcat cggtttcaac atcaatacgc
     3361 ttgccgttat aagcaaagtg ataccaatac aacaacatag aacccaaaca agccattaat
     3421 ttgtcggcaa tgtcacgcgc accagggtga ttgtggtctt ctttttcagg tttcacacaa
     3481 cctaataccg acacacctgt tctcatcaca tccattggat gagaagatgg tggtaattgt
     3541 tctaaagcaa cttttaccgc cgctggcaaa ccacgtaagg cttttaattt ggctttataa
     3601 cctgctaatt cagctgtgtt tggaaatttg ccatgcacca ataaatacgc tacttcttca
     3661 aattcactgg tcgttgcaat atctaaaatg tcataaccac gatagtgtaa atcgttacca
     3721 ctacggccaa cggtacataa agcggtgtta cctgcggctg tgcctgataa agcaacggat
     3781 ttttt
//
LOCUS       DAOQMB010000221         3771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12494_length_3771_cov_17.606566, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000221 DAOQMB010000000
VERSION     DAOQMB010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12494_length_3771_cov_17.606566"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1561
                     /gene="ahpF"
                     /locus_tag="PLY05_07795"
     CDS             17..1561
                     /gene="ahpF"
                     /locus_tag="PLY05_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HNC03243.1"
                     /translation="MLDANLKAQLQAYLERLVNPIELIASLDDSQAAQELHQLLQVIA
                     GLNAKIRLAEPVSGERQPSFAIARVGETPRVRFAGIPLGHEFTSLVLALLQVSGYAPK
                     IEAAIAEQIKGLSTRLNFETYVSLSCHNCPDVVQALNIMAVLNPNISHTMIDGALFQQ
                     EVTDKNIMAVPSVRLNGAEFGQGRMTLEEILAKVDTEAEAKAAAQLHDKAPYDLLIVG
                     GGPSGAAAAIYAARKGIRTGVVAERFGGQVMDTLAIENFISVKETDGAKLARSLEEHV
                     KSYEVDIINNQRASRLSQDDLFHVELASGATLRSKAILIATGARWREMNVNGEKEYRG
                     RGVAYCPHCDGPLFKGKAVAVIGGGNSGVEAAIDLAGIVSHVTLLEFDSKLRADAVLQ
                     KKLYSLPNVTVITSALTTEVHGDGQKMTGLSYTNRLNNESQHLAVAGVFVQIGLLPNT
                     DWLKDTLNLTNRGEIEVNARGETSMAGVFAAGDVTTAPFKQIIIAMGEGAKASLAAFD
                     YLIRQQ"
     gene            1796..3262
                     /locus_tag="PLY05_07800"
     CDS             1796..3262
                     /locus_tag="PLY05_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03244.1"
                     /translation="MAKLLFTILRILTEKLRWLVLIVIVLLAAFWLKHEWQQLEQAEQ
                     QYLQQRQTQIVLNKRYQELKIAQQKTAQATAKLETQLANRQTLVTRLEQELSELRQQR
                     DDLWKEHWLSRKNPLSETAQQLREYKIKIAALTAQLALAKQAQQLWQDKVSNSAIIAE
                     NKRLIEELLQTKQALNHISNTVQHSQHLVNSSLLTQLKQAISEILPMAIGILLAAILT
                     PIIIKIVLYFLVAPIVSRLKAVQIDASIQEPYLPIATGHISQHALTVELLPHQELLLH
                     PDYLQSFSQRAEKTTQWFLNASIPLTSWAAGLVTLVRIRSAETEVVQLASMYHPFEEL
                     VIISLPPNSRLVCKPRGLVGVIKDRNHPVVISKHWRLFSLHSWLTLQLRYLIFHGECQ
                     LIIKGSGGVVVESAQQSRLIQQSATLGFSSDLAYSNYRCETFVSYYLGKDSLFNDRFQ
                     GKTGLYFYEQSPLHQPTQGKASKRVEGIWDALLKVFGI"
BASE COUNT         1162 a          767 c          740 g         1102 t
ORIGIN      
        1 ctactggagt catatcatgt tggatgccaa cttaaaagct caattacaag cctatttaga
       61 acgactggtt aatcccattg aattgattgc cagtttagat gacagccaag ccgcacaaga
      121 attgcatcag ttattgcaag taattgctgg tttaaatgcc aaaattcgtc ttgccgaacc
      181 tgtctctggt gaacgtcaac cttcatttgc gattgcacgt gtgggagaaa cacctagagt
      241 acgttttgca ggcatccctt tagggcacga atttacctct ttagtattag ccttattaca
      301 agtcagcggt tatgccccca aaattgaggc ggcgattgct gagcaaatta aaggcttgtc
      361 tactcgctta aattttgaaa cctatgtttc gttgtcttgt cacaactgtc cagacgtggt
      421 gcaagcctta aacattatgg cggtacttaa ccccaatatc agtcatacca tgattgatgg
      481 ggcgttgttt caacaagaag tgaccgataa aaacattatg gctgtgccat ctgtacgttt
      541 aaatggtgcc gagtttggtc aaggccgaat gactttagaa gaaattttag ccaaagtaga
      601 tactgaagct gaagccaaag ccgcagctca gttacatgac aaagcccctt atgatttgtt
      661 aattgtcggt ggtggcccat caggggcagc ggctgcgatt tatgccgccc gtaaaggcat
      721 tagaacgggt gtcgttgctg aacgttttgg tggccaagta atggatacct tagccattga
      781 aaactttatc tcggtgaaag aaactgatgg cgcaaaatta gcacgttctt tagaagaaca
      841 cgtaaaaagc tatgaggttg atattatcaa caatcaacgt gccagccgtt taagccaaga
      901 tgacttattt catgtggaat tagccagtgg cgcaacctta cgcagcaaag ccattttaat
      961 tgcgacaggt gcacgttggc gcgaaatgaa cgtcaatggt gaaaaagaat accgtggtcg
     1021 tggggtagcc tactgcccac actgtgatgg gccgttattt aaaggcaaag ccgttgcagt
     1081 gattggcggt ggtaattcgg gcgtagaagc agcgattgat ttagcaggta ttgttagcca
     1141 cgtgaccttg cttgagtttg atagcaaact acgtgctgat gcggtgttgc aaaagaaact
     1201 ctatagtttg cctaatgtca ccgtaatcac ttcagcctta accactgaag tacatggcga
     1261 tggccaaaaa atgacggggc taagttatac caatcgctta aacaatgaga gtcagcattt
     1321 agcggttgct ggcgtgtttg tccaaattgg tttattgccc aatactgatt ggttaaaaga
     1381 cacactcaac ttaactaatc ggggtgaaat tgaagtcaat gcgcggggcg aaacctcaat
     1441 ggcgggtgtg tttgccgcag gtgacgtgac gactgccccc tttaagcaaa ttattattgc
     1501 tatgggtgaa ggtgcaaaag cctcattagc agcgtttgat tatttgatta gacagcaata
     1561 aactccgaac ttaggcattg catcgcaatt agcacatttt acaaacaaat aggcgataat
     1621 gcctaagtcc taaatggcag aataggggct aaaatcagct cctattctta attgcgacaa
     1681 atctagtttt ttatgttttt tctgattgag cttggcttta ttttgttagt ttttggtggt
     1741 ttggctttat tggtatggta taaaagtcga aagcaggacg acgattaact taaccatggc
     1801 caagctctta ttcactatac tgcgtattct gaccgaaaaa ctgcgttggt tggtactcat
     1861 tgttattgtg ttactagcag ctttttggct caaacatgaa tggcaacaac ttgaacaggc
     1921 tgagcaacag taccttcaac aacgtcaaac tcaaattgtt ctcaacaaac gctaccaaga
     1981 gctaaaaata gctcaacaaa aaacagctca agccacagca aaattagaaa cacaactcgc
     2041 taatcgccaa accttagtga ctcgcttaga acaagagttg agtgagttgc gtcaacaacg
     2101 cgatgactta tggaaagaac attggttatc acgcaaaaat cctttatctg aaacagccca
     2161 acaactccgt gaatataaaa ttaaaattgc tgctttaaca gcacaactag cccttgctaa
     2221 acaggcacaa caattatggc aagacaaagt cagcaattca gctattattg ccgaaaataa
     2281 acgtctaatc gaagagcttc tgcaaaccaa acaagcctta aatcatattt caaacacagt
     2341 acaacatagt caacatttag ttaatagcag cttattaaca caactcaaac aagccatcag
     2401 cgaaatttta cccatggcta taggcatttt attagctgct atactaaccc ctattatcat
     2461 taaaattgtc ttgtattttt tagtagctcc tattgtctct cgtttaaaag ccgtacaaat
     2521 tgatgctagc attcaagagc cttatttacc gattgcaaca ggtcatatct cacaacatgc
     2581 cttgactgtt gagttactgc cgcatcaaga gttattacta catcctgatt atttgcaaag
     2641 ctttagtcaa cgtgccgaaa aaacaacgca atggttttta aacgcctcta ttccattaac
     2701 cagttgggca gcaggtttag tcactttagt cagaatacgc agtgcagaaa cagaagtcgt
     2761 acaactggcc tcaatgtatc atccttttga agaattggtg attattagcc tccctccaaa
     2821 tagccgtctg gtttgtaaac ctcgtggttt agtaggcgtt attaaagatc gtaatcatcc
     2881 tgttgttatt agcaaacatt ggcgattatt tagtttacat agctggttaa cactacaatt
     2941 acgttatttg atttttcatg gtgaatgtca attaattatt aaaggcagtg gcggtgttgt
     3001 cgtcgaatca gcacaacaat cacgccttat tcaacaaagt gccactttgg gttttagcag
     3061 tgatttagct tatagtaact atcgttgtga aacctttgtg tcctattatt taggcaaaga
     3121 ctctttattt aatgatcgtt ttcagggcaa aacaggccta tatttttatg agcaatcacc
     3181 cttacatcaa ccaacacaag gcaaagctag caaacgagtt gaagggatat gggacgcctt
     3241 attaaaagta tttggtattt aatcgttaaa aaatcaccat tcatcgactg ttcattcaca
     3301 gcaacaatta actctagtag attaaatcaa caagcttata tttactaaaa ttttaacttt
     3361 aaacgccatt tttgatataa aaagtcaaaa ttcatacttt ttcacgccat cattgcgaaa
     3421 agacgccaaa aaatcatcct caaattcatc ctttttttca tccttacggt tgatttttag
     3481 ccttttagtt tagttcatca tacgtcccat caagagcagt aatgctcata aaaaaaccta
     3541 aaacaaccat aacaaactca aaaactcagg cattttcaac atcccaattt tgagatgctt
     3601 agagaatgag ttgacaggat gacaaccatg aaaaaattat taacgctttc tgccgtcgct
     3661 ttagctagct cattaatggt cgctgcacca accgcgcaag ctggttacac tacaaccaaa
     3721 tatcctactt tattagtaca cggtatttta gggtttaaaa atttttgggg t
//
LOCUS       DAOQMB010000222         3768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12509_length_3768_cov_15.452734, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000222 DAOQMB010000000
VERSION     DAOQMB010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12509_length_3768_cov_15.452734"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLY05_07805"
     CDS             complement(<1..746)
                     /locus_tag="PLY05_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC03245.1"
                     /translation="MTLPITQTLLVTEDKGILHITLNRPESRNSMSLQMVQELQALFT
                     WGAENHIRAVVLRGAGGHFCAGGDIRDMMTIRQQAVENPHAYQNFNRTFGHLLTQVNN
                     APFLVITVIEGAVLGGGFGLTCVSDVALAKADAQFGMPETRLGIIPAQIAPFIVQRLG
                     LTQARRLALLGMRINGQQAQAMGLVHEVYADNDSLETALARTINDLRQCAPNATQVTK
                     ALLHQVGEQPLNQVLDDAAEAFACAIQSAEG"
     gene            complement(951..2114)
                     /locus_tag="PLY05_07810"
     CDS             complement(951..2114)
                     /locus_tag="PLY05_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC03246.1"
                     /translation="MKFTPEHDALRRTTKQFVDSQINPFVDEWEEAGIFPAHELFRKM
                     AELGLLGVCKPEENGGMGLDYSYNMVVTEELGKINCGGVPMAIGVQTDMATPALARFG
                     SKELRDEFLTPAIAGEYVASIAVSEPHAGSDVAAIKTTARKDGDDYIINGTKMWITNS
                     TQADFLCLLANTSDDKPHVNKSLIVVPTKAAGVSFSKKLNKLGMRSSDTAQIFLDNVR
                     VPQRYVIGAEGMGFMMQMMQFQEERLYGAANAVGGLESCIDKTIAYCRERTTFGQALI
                     DNQSIHFRLAELRTEVECLRALTYQACEQHISGQDVTLLASMAKLKAGRLMREVTDSC
                     LQYWGGQGFMWENPIARAYRDGRLVSIGGGADEIMLGIICKLTNTLPSKKKKA"
     assembly_gap    2127..2226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2227..>3768)
                     /locus_tag="PLY05_07815"
     CDS             complement(<2227..>3768)
                     /locus_tag="PLY05_07815"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HNC03247.1"
                     /translation="ADYQANCQAMLKQIDEVAAIKQKVLERSLQEKPKFTKRGKMLPH
                     ERVQHLLDAGSPFVELCGLAGYMLDDDKDGTEAGGNMIAGIGVINGVRCLVGASNSAI
                     KGGTISPSGLRKSLRLQQIARQNKLPMVTLAESGGANLNHAADIFVEGARSFANQARL
                     SAMGIPQITVVHGNATAGGAYQPGLSDYMVVVRGKTEMFLAGPPLLKAATGEVATEED
                     LGGAAMHAQVAGTAEYLAENDADGIRQARDIVSMLGWDKAQLNHDAPEPRYPIDDILG
                     IIPCDLRKPYDVRQVIACLVDDSNFLDFKTEFDNQTICGWANIGNVTIGIIGNNGPIT
                     PQGAAKASQFIQLCDQTNRPLLFLHNTTGFMVGTESEQNGVIKHGSKMIQAVANTRVP
                     KLSIVIGGSYGAGNYAMCGRGLDPHFIFAWPNSKTAVMGGAQAGKVLRIVTEAKQQKM
                     GITPDENVLNFLEMSTAAKLESQSTALFNTAKLFDDGVIDPRDTRKLLVFLLQTIKEA
                     EQRQL"
BASE COUNT         1013 a          852 c          772 g         1031 t
ORIGIN      
        1 ccttcggcac tttgaatggc acaagcaaag gcttctgcgg catcgtctaa tacttggttt
       61 aaaggctgtt cgccaacttg gtgtaataag gctttggtca cttgtgtggc attgggtgca
      121 cactgacgta aatcattaat agtgcgagca agggcggttt ctaagctgtc attatcggca
      181 tagacttcat gcaccaaacc catcgcttgg gcttgttgac cattgatacg cattcccaat
      241 aacgccaaac gtcgtgcttg ggttagcccc aaacgttgaa caataaacgg tgcaatttga
      301 gcaggaataa tgcctaagcg agtttctggc ataccaaact gtgcatcggc tttggctaag
      361 gcaacatcac taacacaggt caaaccaaaa ccaccaccta agactgcacc ttcaatgacc
      421 gtgatgacta agaatggcgc attattgact tgcgtcaata agtgaccaaa ggtgcgatta
      481 aaattttgat aggcatgagg attttcaaca gcttgttgac gaatggtcat catgtcacga
      541 atatcaccac ccgcacaaaa atgtccacct gcaccgcgta agaccacagc acgaatatga
      601 ttttctgcac cccatgtaaa tagggcttgt aactcttgca ccatttgcaa actcatggag
      661 ttacggcttt ctgggcgatt cagggtgata tgtaaaatgc ctttatcttc agtgactaat
      721 aaagtttggg tgataggtaa tgtcatagtt ttttcctaaa tggtaacggc gtaggatggg
      781 ttgaggtacg aaacccatct ttttaggact tacgcggtta tcgcttattt gctcacattt
      841 aaatcttttt taacgatttt atgctcgcaa aacgcgctaa tttcggtgaa atgcgtaagt
      901 cctacttgta tgttgtgatg ggtcaacgac ccatcctacg agttgcacaa ttaagctttc
      961 ttctttttgc taggtaaggt attggttaat ttacaaatga tgccaagcat aatttcgtca
     1021 gctccaccac caatagacac caaacgaccg tcacggtaag cgcgagcaat ggggttttcc
     1081 cacataaagc cttgaccgcc ccaatactgt aagcagctat cagtgacttc gcgcattaaa
     1141 cggcctgctt ttaatttagc catggaggct aataaagtga cgtcttgacc actaatgtgt
     1201 tgttcacaag cttggtaggt gagtgcgcgt aaacattcca cttcagtgcg taattcagct
     1261 aaacggaagt gaatggattg attatcaatt aaagcttgac cgaacgtggt gcgctcacgg
     1321 caataggcaa tggttttgtc gatacagctt tccaaaccac ccaccgcatt ggccgcaccg
     1381 tataaacgtt cttcttggaa ctgcatcatt tgcatcataa agcccatgcc ttccgcacca
     1441 atcacataac gttgtggcac acgcacgttg tctaagaaaa tttgagcagt atcagaagaa
     1501 cgcatgccta atttattcaa ctttttggag aatgacacgc cagcagcttt agtgggtacg
     1561 acaattaagg acttgttaac gtgaggttta tcatcactgg tattggctaa caaacacaaa
     1621 aagtctgctt gtgtggagtt ggtaatccac atttttgtac cattgatgat gtaatcatca
     1681 ccatctttgc gtgcggtggt tttaatggct gccacatcag aaccagcatg aggttcagac
     1741 acagcaatcg aggcaacata ttcgcctgca atagcaggag ttaaaaactc atcacgtaat
     1801 tctttagaac caaaacgggc aagggcaggt gtggccatat cggtttgtac cccaattgcc
     1861 atcggtacac caccacaatt aattttgcct aattcttcgg taacgaccat gttgtaagag
     1921 tagtctaaac ccataccgcc gttttcttca ggcttacaca cacccaataa acccaattca
     1981 gccatttttc taaataactc atgggctggg aaaatacctg cttcttccca ttcatcgaca
     2041 aaaggattga tttgactatc aacaaattgt ttggtggtgc ggcgtaaagc gtcatgttct
     2101 ggggtaaatt tcatcttgct gtcctcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnttaa ctggcgttgt tctgcttctt taatggtttg taataagaaa acgagaagtt
     2281 tgcgggtgtc tcgtgggtca atcaccccgt catcaaacag ttttgcagta ttaaacaaag
     2341 cagtggattg cgactctaat ttggcagcag tactcatttc taagaaatta agcacatttt
     2401 cgtcaggggt aatgcccatt ttttgttgtt tggcttcagt gacaattctt aatactttgc
     2461 ctgcttgtgc gccacccatc acggctgttt tcgagtttgg ccatgcaaaa ataaaatgag
     2521 ggtctaaacc acggccacac atggcatagt tacctgcacc ataagagcca ccaatcacaa
     2581 tagatagctt cggtacacgg gtattggcca cggcttgaat catttttgag ccatgcttaa
     2641 tcacgccatt ctgctcagat tctgtaccta ccataaagcc tgttgtattg tgtaaaaaca
     2701 ataatgggcg attggtttgg tcacaaagct gaataaattg gctggctttg gcagcgcctt
     2761 ggggagtgat tgggccgtta ttgccaatga tgccaatagt tacgttaccg atattggccc
     2821 aaccacaaat cgtttgatta tcaaattcgg ttttaaagtc taaaaagtta gagtcatcga
     2881 ctaaacaggc aatcacctga cgtacatcat aaggcttgcg taaatcacaa ggaataatgc
     2941 ctaaaatgtc atcaatcgga taacgtggct ctggtgcatc atgattcagt tgtgctttgt
     3001 cccaacctaa catcgagaca atatcacgtg cttggcgaat gccatcagca tcgttttcgg
     3061 ctaaatattc cgccgttcct gcaacttgag cgtgcattgc cgcaccgccc aaatcttctt
     3121 ctgtcgccac ttcaccagtt gccgctttta atagcggagg gcctgccaaa aacatttcgg
     3181 ttttgccacg caccaccacc atataatcgg ataaacccgg ttgataagca ccaccagccg
     3241 tcgcattacc atggacaacg gtaatttgcg gaatacccat tgctgataaa cgggcttggt
     3301 tagcaaaaga acgcgcacct tcgacaaaaa tatcggcagc atgattgagg tttgcaccac
     3361 cactttcggc caacgtgacc attggcaatt tgttttgacg ggcaatttgt tgcaaacgta
     3421 agcttttacg caaaccactg ggcgaaatcg tgccgccttt gatggcagaa ttacttgcac
     3481 ccaccaaaca acgcacacca ttaatcacac caatgcctgc aatcatattg ccgccagctt
     3541 cggtgccatc tttgtcatca tcgagcatat agcctgctaa accacacaac tccacaaatg
     3601 gcgaacctgc atctaatagg tgttgtacac gttcatgagg caacattttg ccacgcttag
     3661 taaatttggg cttttcttgt aaagaacgtt ctaaaacttt ttgtttaatc gctgccactt
     3721 cgtcaatttg tttgagcatg gcttggcaat tggcttgata atcagcac
//
LOCUS       DAOQMB010000223         3752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12597_length_3752_cov_15.549905, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000223 DAOQMB010000000
VERSION     DAOQMB010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12597_length_3752_cov_15.549905"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..>1201
                     /gene="glyA"
                     /locus_tag="PLY05_07820"
     CDS             81..>1201
                     /gene="glyA"
                     /locus_tag="PLY05_07820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNC03248.1"
                     /translation="MFSNINLAQFDPDLAQAISQEDARQEAHIELIASENYCSPLVMA
                     AQGSKLTNKYAEGYPGKRYYGGCEYVDIVESLAIERAKKLFGADYANVQPHSGSQANG
                     AVFLALLNAGDTVLGMSLAHGGHLTHGAKVNFSGKSYNAVQYGLNPTTGEVDYDEVER
                     LALEHKPKMIIAGFSAYSRIMDWQRFRDIADKVGAYLMVDMAHVAGLVAAGVYPNPVQ
                     IADVTTTTTHKTLRGPRSGLILARKNEEIEKKLNSAVFPGNQGGPLMHVIAAKAVCFL
                     EAMQPSFVDYQKQVVKNAQTMAAVFIERGYDVVSNGTDNHLFLVSLVGKELTGKEADA
                     ALGKAGITVNKNAVPNDPRSPFVTSGIRVGTPAVTTRGFA"
     assembly_gap    1202..1301
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1447..2565
                     /locus_tag="PLY05_07825"
     CDS             1447..2565
                     /locus_tag="PLY05_07825"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HNC03249.1"
                     /translation="MRYSVLWPVLLTGLLAGCLSEQKETNAVSNTTNANTTQMVALGD
                     KLFHDVSLSSQGNQACASCHNVNQAFSDADQTMPVSKGSEVGQFGTRNTPAASYTRFI
                     PDLHQEQEDGETLWKGGLFLDGRVNSLEEQAQKPFLNPAEMNNKDAADVVAKLRASTN
                     ANLFREVFGSDALDIGKEDQAFGQMAQAIAAFERTPVFSPFSSKFDFYLQGKAQFTTL
                     ELQGMSLFIRADKGNCAACHPVTASGDGTPALFTDFSYDNIGVPRHPNRQFFTENFVD
                     EGLAKTTNDPMLRGQFRVATLRNIAKTAPYMHNGRFDNLREVVEFYNTRDVEPQRWGE
                     PEVPETVNRDELGNLRLTDREIDAIVAFLNTLSDGYQP"
     gene            2583..3749
                     /locus_tag="PLY05_07830"
     CDS             2583..3749
                     /locus_tag="PLY05_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC03250.1"
                     /translation="MFRPLLTALTVAIFASHSYAESLDDLRAELATQKAQIAAQQSQL
                     EALATAVENQKATASNSATTVGFYGEVHYNSFKEPDPAIGKNNVHAHRAVILLSHAFN
                     DNLRFYSEVEFEGAADETEIETELEQLFIDWRVHPKLALNIGQFLLPVGLLNESHEPN
                     VFYGVERNPVEEKIIPATWWEKGVMVRAVPAEGLAVDVAVHNGLRGDINTLGGADGLR
                     EFRQEFGGSRAEDLAYTLRVKYQVINGLELAASFQQQENITQSHDLVVGGKASAQLMT
                     AHVDYHWQGFGLRVLGAQWDIDNAVAKATGADKLAGFYVEPSWKVTDKVGVFARYNQW
                     NTAANQVGKSDDEQLNVGVNYWIDPRVVLKADIQNTNQKNKAGDGFNLGVGLSF"
BASE COUNT         1052 a          659 c          834 g         1107 t
ORIGIN      
        1 tgagagaaaa tcattatcgt ttatcaataa gcatgttaaa atataaaatc taatttccct
       61 ccttgtcttg agactctgtg atgtttagca atatcaatct tgcccaattt gaccccgatt
      121 tagcccaagc tattagccaa gaagatgcac gccaagaagc gcacatcgag ttaattgctt
      181 ccgaaaacta ctgctcaccc ttagtcatgg ccgctcaagg gtctaaatta accaataaat
      241 acgccgaagg ttatccaggt aagcgttatt atggcggttg tgagtatgtt gatattgttg
      301 aaagtttagc gattgaacgc gccaaaaaat tgtttggtgc tgactatgcc aacgtacaac
      361 cacactcagg ctctcaagca aacggtgcgg tatttttagc cttattaaat gcgggtgaca
      421 ccgtattggg tatgagctta gcacatggtg gtcacttaac tcacggtgca aaagtaaact
      481 tctctggtaa gtcctataac gcggttcagt atggtttaaa ccccacaact ggcgaagttg
      541 attatgacga agttgaaaga ttagccttag aacacaagcc aaaaatgatt attgcaggtt
      601 tctctgcgta ttctcgtatt atggattggc agcgtttccg tgacatcgct gataaagttg
      661 gtgcgtattt aatggttgat atggcacacg ttgctggttt ggttgctgcg ggtgtttatc
      721 caaacccagt acaaatcgct gatgtgacca ccacaaccac tcacaaaacc ttgcgtggcc
      781 cacgttctgg tttgattttg gcacgtaaaa acgaagaaat cgaaaagaaa ttaaactctg
      841 cggttttccc aggtaatcaa ggtggcccgt taatgcacgt gattgctgca aaagcggtgt
      901 gtttcttaga agcgatgcaa ccttcatttg ttgattacca aaaacaagtg gttaaaaacg
      961 cccaaacaat ggctgctgtg tttattgagc gcggttatga tgtggtttct aatggcacag
     1021 acaatcactt atttttagtg tccttagtag gtaaagagtt aacaggtaaa gaggctgatg
     1081 ctgctttagg caaagctggt attactgtta acaaaaacgc tgtaccaaac gacccacgtt
     1141 caccatttgt gacgtctggt attcgtgttg gtacaccagc agtgacaaca cgtggttttg
     1201 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaaagcta aataaaaaaa
     1321 gacgctatat gcgtcttttt ttatttatat aaaccttatt ttttttgtgg ttttatgata
     1381 aatgtattta caaatataaa cgataactat tatcattaat aatatattta tgttgggtaa
     1441 tggtatatgc gttattcggt tttatggcct gtgttattaa caggactttt agcaggctgt
     1501 ttatcagagc aaaaagagac aaatgcggta agcaatacga caaatgccaa taccactcaa
     1561 atggtggctt taggcgataa gctttttcat gatgtttctt tgtcgagtca aggtaatcaa
     1621 gcttgtgcct cttgtcacaa tgtcaatcag gctttttcag atgctgatca aacaatgcct
     1681 gtgtctaaag gctctgaagt agggcagttt ggtacacgta atacacctgc tgccagttat
     1741 acccgcttta ttccagatct tcatcaagaa caagaagatg gcgaaacctt gtggaaaggt
     1801 ggtttgtttt tagatggccg cgtcaatagt ttagaagaac aagcccaaaa gccgttttta
     1861 aatcctgccg aaatgaataa taaagatgct gcggatgttg ttgccaaact acgtgccagt
     1921 accaatgcca atttatttcg tgaggtgttt ggcagtgatg ctttagatat tggcaaagaa
     1981 gatcaagcat ttggtcaaat ggcacaagcc attgccgctt ttgaacgcac acctgttttt
     2041 agcccattta gctcaaagtt tgatttttat ttacagggca aagcgcaatt caccacctta
     2101 gagttgcaag gcatgagttt atttattcgt gcagataaag gcaattgtgc tgcttgtcat
     2161 cctgtcacgg ccagtggcga tggaacaccc gcattattca ccgattttag ttatgacaat
     2221 attggtgtac cacgtcatcc aaaccgtcaa ttttttacag aaaattttgt tgatgagggt
     2281 ttagctaaaa ccaccaatga tcccatgtta cgtggccaat tccgtgtcgc gacactacgt
     2341 aatatcgcta aaactgcacc ctatatgcac aatggtcgtt ttgacaactt acgtgaggtg
     2401 gttgagtttt ataacacgcg tgatgttgag cctcaacgtt ggggtgaacc tgaagtccct
     2461 gaaacagtga atcgtgatga gctagggaat ttgcgtctaa ctgaccgaga aattgatgcg
     2521 attgttgcct ttttaaatac tttatctgat ggttatcagc cttaaaaacc ttggagttta
     2581 ttatgtttcg tcccttatta acggcattaa ctgtagccat ctttgctagt catagttatg
     2641 cagagtcttt agacgattta cgtgctgagt tagctacgca aaaagcgcaa attgctgcac
     2701 aacaaagtca attagaagca ttagcaacgg ctgttgaaaa ccaaaaagcg actgcaagca
     2761 atagtgcaac gacagtcggt ttttatggcg aagtgcatta caacagcttt aaagagcctg
     2821 atcctgccat tggtaaaaat aatgttcatg cccatcgtgc agtgatttta ttaagccatg
     2881 cctttaatga taatttacgc ttttatagtg aagttgagtt tgagggagca gcagacgaaa
     2941 ctgaaattga aacggaatta gagcaattat ttattgattg gcgtgtacat ccaaaattag
     3001 ccttaaatat tggtcagttt ttattacctg tcggtttatt aaatgaaagc catgaaccta
     3061 atgtgtttta tggtgttgag cgtaaccctg ttgaagagaa aattattcct gcaacatggt
     3121 gggaaaaagg cgtgatggtg cgtgctgtgc ctgctgaagg cttagcggtg gatgtggctg
     3181 tgcataatgg tttgcgtggt gatattaaca ccttgggtgg ggcagatggt ttgcgtgagt
     3241 tccgtcaaga gtttggtggt tcacgtgctg aagatttagc ctataccttg cgggtaaaat
     3301 accaagtgat taatggctta gaattggcgg ctagttttca acaacaagaa aatattaccc
     3361 aaagccatga tttagtcgtt ggtggcaaag catcggcaca gttaatgacg gcgcacgttg
     3421 attatcattg gcaaggtttt ggtttgcgtg tgttgggtgc gcaatgggat attgataatg
     3481 ctgttgctaa agcgacgggt gctgacaaat tagcaggctt ttatgttgag ccatcatgga
     3541 aagtgactga taaagttggt gtttttgcac gatataatca atggaatacg gctgctaatc
     3601 aggtgggtaa aagcgatgat gagcagttaa atgttggcgt aaattattgg attgatccgc
     3661 gtgttgtctt aaaagcggat attcaaaata ccaaccaaaa aaataaagca ggtgatggtt
     3721 ttaatttagg ggttggtttg agcttctaag aa
//
LOCUS       DAOQMB010000224         3720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12773_length_3720_cov_17.876398, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000224 DAOQMB010000000
VERSION     DAOQMB010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12773_length_3720_cov_17.876398"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3432
                     /locus_tag="PLY05_07835"
     CDS             <1..3432
                     /locus_tag="PLY05_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03251.1"
                     /translation="GRPFGDGLAPKRTAASKASIAYQFTSFDIVNSCFSEISAQAANV
                     GICWQSVQKHISFNGRPLKNGIALTPKYNGKVFVIASEQAYKKHGQVVGYYPIITFKT
                     QKHGGITEVFNGLEAAQDYYNRLQGNSYLSGNNPIRIDWEAKRQAEQRAKLAEEKAAK
                     EEAAYKAKRLKDFADVFNTLPREVGDHAYLVKKFGDLAPQAAQSIDLRRGKDYMGDFI
                     AYALYDESMSVCGYQRIYAKIPKGREDNKDIIGKAKGAFALVGDAKKIETRANIAEGL
                     ANAITYHLATDKPCFVALFANNLKLVADFIRKHYADSISNITMIADNDRKNLPINGNI
                     GVYKAIQAINYGNDSKDDYLIIPSCDKPINWDFSDVLVNNDYDIKAVLSAIDNKDNRY
                     YPNRDPIESRLNCIELMKKSQREKSIKSLLAQAVKLAPMRPIDSSLNTIKARLSDDYS
                     ALIDSLTHWVNKLYNAKKEAVKPLFSFTTKLPIFFSQQNIAKINAQRLLDVHGFYVDC
                     RGTGEGKTLLMAEIAAQAKKKKLRVGYIAHRVSLIANSAKRLELENYQDLKPFDMPDV
                     QGSAICANSLDKLYFSRFFDNCDILFIDEVKQVLEHVLYGTMKSESRAKVLESIKYAI
                     QNAKLVICADADLDQETLDWLNSCRPVTHEIKSDLSGKPPKVDKTVFFGKYDIIQTSA
                     IHSVLSGKKTLIQCDTKTETEVMAKLLTDKGVNADDILIINRDTKAEALEAEFLKDPN
                     AYLAQYQPRVVLASPTISSGFSIELQGAFDAVYLLMTGILTPTEIMQTSARYRLVKEL
                     FIGFSEQNSNRKPTTTEAQKLFGDMVIRGKIRAFFDKETDNLLIEAQPSELDKWFYKL
                     STKRELSLKDYANKTLLCFEAKGYTVKPFVNDESSTVSKVDEIKTLSKQAKKAVKEER
                     IFSIINADAISKDTANQYEKITDKLNLKNKRELTRFKMSEALATESLKVTDVEFCENS
                     GFKKLSRFEIMQSIEADCIEFDKYQAQTKTDLSQMTKATEQNSIYKMLFETLGINQQS
                     GEGSFTHTQAVKALKELQTRHNELAALGLGNFEQVKEKTAIRSIGYLLDKLGLNTAAF
                     KNKGKRHYMIEPDSWECMTNYAKQRKAKNIHTLKTGQLNNGNVFFFWTDKMQKCA"
BASE COUNT         1303 a          713 c          741 g          963 t
ORIGIN      
        1 gggcgaccat ttggcgatgg cttagcccct aagcgaacag cagcaagcaa agcaagtata
       61 gcatatcaat tcaccagttt tgatattgtt aattcctgtt ttagcgaaat ctcagctcaa
      121 gcggcaaatg ttggcatttg ttggcaatcg gtacaaaagc acatctcatt taatggcaga
      181 cccttaaaaa atggtattgc cttaacgcca aaatataacg gcaaagtctt tgttattgcg
      241 tcagaacagg cttataaaaa gcatggtcaa gttgtcggtt actatccgat aatcactttt
      301 aaaactcaaa agcatggcgg cattaccgaa gtttttaacg gattagaagc ggcacaggat
      361 tactacaacc gattgcaagg caatagttac ttatcaggta acaatccgat aagaatagac
      421 tgggaagcca aacgccaagc ggaacaaaga gccaaactag ccgaagaaaa agcagcaaaa
      481 gaagaagccg cttacaaagc aaagcgttta aaagattttg ctgatgtttt taatacttta
      541 ccgcgtgaag ttggcgacca tgcttacctt gttaagaagt ttggagacct tgcaccacag
      601 gccgcgcaat caatcgacct tagacgcggt aaggattaca tgggggattt tatcgcctac
      661 gccttgtatg acgaaagtat gagtgtatgt ggctatcaga gaatttatgc caaaataccc
      721 aaaggccgcg aagataacaa agatattata ggcaaagcta aaggcgcatt tgctttagta
      781 ggtgatgcca agaaaatcga gactagagca aatatagccg aaggtttggc caatgcaatc
      841 acttatcact tagcaacaga taaaccgtgt tttgttgcgt tattcgctaa taatctaaaa
      901 cttgttgctg attttatccg taagcactac gcggacagca ttagcaatat aaccatgatt
      961 gcagataatg accgtaaaaa cttgcccata aatggcaata taggcgttta taaagctatt
     1021 caagcaatca attatggcaa cgattcaaaa gacgattatt taataattcc gtcttgtgat
     1081 aagccgataa actgggattt tagcgatgtt ttagtaaata atgattacga cattaaagcc
     1141 gttctatctg ccattgataa caaagataat cgttattacc cgaaccgtga cccgatagaa
     1201 agccgattaa attgcattga gttaatgaaa aaatctcagc gcgaaaagag tataaaaagc
     1261 ctattagctc aagccgtaaa acttgcgcca atgcgaccaa tagacagctc actaaacacg
     1321 attaaagcgc gtttaagcga tgattacagc gcgttaatag attcccttac tcattgggta
     1381 aataaactct acaacgcaaa aaaagaggcc gtaaaaccac tttttagctt taccactaaa
     1441 ttacctattt tcttttctca acaaaacatt gccaagatta acgctcaacg attgctagat
     1501 gttcacggtt tttatgttga ttgcagaggg acaggcgaag gtaaaaccct attaatggcc
     1561 gaaatagcag cccaagccaa aaagaaaaaa ttaagagttg ggtatattgc ccaccgtgta
     1621 agcctgattg ccaattctgc aaaacgcctt gaattagaaa actatcaaga cctaaaaccg
     1681 tttgatatgc ctgatgttca aggttctgct atttgtgcga actcattaga taagctctac
     1741 ttttcaaggt tttttgataa ttgcgatatt ttatttattg atgaagtaaa gcaagtttta
     1801 gaacacgtcc tttacggaac aatgaaatca gaatcacgcg ccaaagtgtt ggagagcatt
     1861 aaatacgcaa tacaaaacgc taagctagtt atttgtgctg atgcggattt agaccaagaa
     1921 acactagact ggcttaactc atgccgacct gtcacccatg agataaaaag cgacctaagc
     1981 ggaaagccgc caaaagttga taaaaccgtg ttttttggta aatacgacat aatccaaacc
     2041 agtgctattc actcggtatt atcaggcaaa aaaacgctta tacaatgcga caccaaaacc
     2101 gaaaccgaag ttatggccaa gttgctcaca gacaagggcg ttaatgctga tgatattctg
     2161 ataatcaaca gagacaccaa agccgaagcc ttagaagcag agtttttaaa agaccctaat
     2221 gcctacttag cccaatatca gccgcgtgtt gtgttggcta gtccgactat ctcaagcggt
     2281 tttagtattg aattacaagg cgcgtttgat gccgtctatt tgctgatgac aggtatttta
     2341 acgcctactg aaattatgca aacctcagcg cgttatcgcc ttgttaaaga gctatttatt
     2401 ggttttagtg aacaaaacag caatagaaag ccaaccacca ccgaagcgca aaagttattc
     2461 ggtgatatgg tgattagggg caagattaga gcgttttttg ataaggagac agataattta
     2521 ttgattgaag ctcaaccgtc agaattggat aaatggtttt ataaactcag caccaagcgc
     2581 gaactaagcc tcaaagatta cgccaataaa accctacttt gttttgaggc caaagggtac
     2641 acggttaaac catttgtgaa tgatgaatcg tcaaccgtat caaaagttga tgaaataaaa
     2701 acgctgtcca aacaagcgaa aaaagccgtt aaggaagaaa ggatattttc tattatcaat
     2761 gctgatgcca ttagcaaaga cacggcaaac cagtacgaaa aaatcacgga taaactcaac
     2821 ttaaaaaaca agcgcgaact aacacgcttt aagatgagtg aagccttagc aacagaatcg
     2881 ttaaaagtca cagacgttga gttttgcgaa aactcaggat ttaaaaagct gtcacgattc
     2941 gagattatgc aaagcattga agctgattgc attgagtttg ataaatacca agctcaaacc
     3001 aaaacggatt tatcacaaat gaccaaagcg acagaacaaa acagcattta taagatgcta
     3061 ttcgagacgc ttggtataaa ccaacaatcg ggcgaaggtt cttttaccca tactcaagct
     3121 gtcaaagcat taaaggaact gcagacacgg cataacgagt tagcagcatt aggactaggc
     3181 aattttgagc aagtcaaaga aaagaccgct atacgctcaa tcggttatct attggataaa
     3241 ttgggactaa atacagccgc ttttaaaaac aaaggtaaac ggcattacat gattgagcct
     3301 gatagttggg agtgtatgac taattacgcc aaacagcgaa aggcgaagaa tatacacacc
     3361 ctaaaaacag gacagttaaa taacggtaat gttttctttt tttggacaga taaaatgcaa
     3421 aaatgcgcct aaatgatggt tcgggacaga taaaatgagt attttgttta aaagagaaaa
     3481 tttatctgtc ccgacaatga agcaaaaccc aaaggattgc agtattttta tgcccttaat
     3541 gatgtcagat aaatcatggt ttttagtttt tggagcagta cgcgcgtatt gtacggaaaa
     3601 ctcaagcaca cgtttataag taaacttttg ttagctttgg caaacaaaac cttgccgtaa
     3661 tatagccgta atctcaccct tttttatagc gttcaaaaac tggcaatata aacaaaatag
//
LOCUS       DAOQMB010000225         3701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12878_length_3701_cov_13.075425, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000225 DAOQMB010000000
VERSION     DAOQMB010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12878_length_3701_cov_13.075425"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /gene="ispH"
                     /locus_tag="PLY05_07840"
                     /gene_synonym="lytB"
     CDS             <1..585
                     /gene="ispH"
                     /locus_tag="PLY05_07840"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394089.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC03252.1"
                     /translation="GHPEVEGTMGQYDTQFGGRIYLVEDEEDVAKLVVNNPQQLAFVT
                     QTTLSVDDTARVIDALRLKFPAIQGPRKDDICYATQNRQDAVKTLAQNCDIVLVVGSP
                     NSSNSNRLRELAERMGSKAYLIDNAEQLQKEWFDSTQKIGLTAGASAPEILIQQVVQR
                     LQQWGGQTPQEITGREENVVFSMPKELRIPSIAL"
     gene            853..1374
                     /locus_tag="PLY05_07845"
     CDS             853..1374
                     /locus_tag="PLY05_07845"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNC03253.1"
                     /translation="MSKQWGFTLVEVMVSIAVIAVIVVFAFPAMQLMQANSRMASVAN
                     DLAADLKNARSRAIVTRSNITFSANTGGWSNGWSASFQVNQSDSSNQKVSVLVLEKLQ
                     LPAGISVTQSVSPLTFNGATGRVADSSNASVDVVFRVCDTKISKETGSNVLLNRFGRV
                     LIQRHNDDSTCNQ"
     gene            1387..1896
                     /gene="pilV"
                     /locus_tag="PLY05_07850"
     CDS             1387..1896
                     /gene="pilV"
                     /locus_tag="PLY05_07850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNC03254.1"
                     /translation="MFNQFPALNKQKGFGMTEVIVSMLVLGVAVIGFSALQVRALDVT
                     GDSMFRTQAIALAQDLGERIMLNPNGATKYKATWNATNSSDCETASCTPEQMAEYDIK
                     SVTNTAKQILPNGQIKLIKCIERNNYCVYVTWNKTQPNAEGAADSCSNENDTFVTNAD
                     CVKLETSLL"
     gene            1893..2789
                     /locus_tag="PLY05_07855"
     CDS             1893..2789
                     /locus_tag="PLY05_07855"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNC03255.1"
                     /translation="MMTQYSKGFTLIELMVSLTLALIIMLSAMQLLLTNQMSFNLQSG
                     MGNVQENGRFALDYINSTVRSAEYSQNIATPTTGIIMFKDELPSDAANKIEDSHVTNN
                     DITLIETQVSPGVTKNKGYSDQLVTRMWISQAMIDGGFRDCEGNAVTTPGFVVTRYFV
                     RDDLTSNKEALACDAGFYQADAASVSNYKDNGVVLLSTIDSFQVLFGVSAFPLPALGQ
                     RFPVQYMTAKAYTDIPVASRPAIVSLRIGLVVRSNEKVNTGFNSTPEKITVLDKEISG
                     SNLDDKFVHRLFTSTLALRNAI"
     gene            2800..3486
                     /locus_tag="PLY05_07860"
     CDS             2800..3486
                     /locus_tag="PLY05_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03256.1"
                     /translation="MNKTPIKTLQQGMALISVLVILLIVTLLGVTAMRMGLSGLMLAN
                     NSQVSQLLFQSADMGTVQLTNTINENILDSMDVTGVIGNASGADTHLCLKPTTTGTNF
                     KYFKAGACDPTSSSDYLSKREVVLTQVSYSRRTITDGESSNDLDDISLTGSSISVTGA
                     AEKLKTFSTAVIPSFGTVSASVIKNCLNKANADDEESDNASAETITDCLTDVGAVFTT
                     HATEYRIQRQ"
     gene            3503..>3701
                     /locus_tag="PLY05_07865"
     CDS             3503..>3701
                     /locus_tag="PLY05_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03257.1"
                     /translation="MKNNKSISFKKNYLATAIAYVYVSLAVAPVYASDTEIYVDSTQS
                     SAVSPNLMMVFDTSGSMAECVD"
BASE COUNT         1171 a          578 c          798 g         1154 t
ORIGIN      
        1 ggacatcccg aagtggaagg caccatgggg cagtacgaca ctcaatttgg tggtcgtatt
       61 tatttagtag aagatgagga agatgttgct aaattagtgg taaacaatcc acaacagtta
      121 gcattcgtca cacaaacaac cttatcggta gatgacaccg cacgagtgat tgatgcttta
      181 aggctaaagt ttccggcgat acaaggcccg cgcaaagatg atatttgtta tgccactcaa
      241 aatcgtcaag acgctgttaa aacactcgcc cagaattgtg atattgtgtt agttgtgggc
      301 tcaccaaatt cctcaaactc taatcgttta cgtgaattgg ctgagcgtat gggttctaaa
      361 gcctatttga tagataatgc agaacaatta caaaaagaat ggtttgattc aacacaaaaa
      421 atcggtttaa cagcaggtgc ttcagcgcct gaaatattga ttcaacaagt tgtgcaacgt
      481 ttacaacaat ggggcggtca gaccccacaa gaaatcacag gaagggaaga aaatgtggtc
      541 ttttcaatgc ctaaagagct acgaattcct tctattgcat tataaaaaca atttaaattc
      601 atatccaaag ggtgacattt attgtcactc tttgacgcct attgtctaac aaaaaatgta
      661 acaaaatatt aaaaatgacc tcttggtcaa ttaaaaatag ccgactgtcg cgttctgctt
      721 gaggagtggt tatttaatgt ctaatgttca cttaacaaag tcggtactgt gtcaggtact
      781 gttaaattag acactaaata gggctttaat aaatataaga tcagttctaa tttaagtgtt
      841 atggggtgtg tgatgtctaa acagtggggt tttacattag ttgaagttat ggtgtccata
      901 gctgtgatag cagttatagt agtctttgct tttcctgcaa tgcaattgat gcaggcaaac
      961 tcacgcatgg catcagtagc taatgatttg gcagcagact taaagaatgc tcgttctaga
     1021 gcaattgtaa ctcgtagcaa tattacattt agtgcgaata caggcggatg gtcaaatggc
     1081 tggtctgctt cttttcaggt aaatcaatct gatagtagta atcaaaaagt atctgttctt
     1141 gttttggaaa agcttcaatt gccagcaggt ataagtgtta cacaatcggt gagtccattg
     1201 acgtttaatg gtgctacagg acgggttgct gatagtagca atgcttccgt ggatgttgtt
     1261 tttagggttt gtgataccaa aatttctaaa gaaactggtt ctaatgtatt gttgaatcgt
     1321 tttggtcgcg tgttaataca acgccataat gatgactcta cgtgtaatca ataaaaggag
     1381 ataagaatgt ttaatcaatt tcctgcatta aacaaacaga aaggttttgg tatgacagag
     1441 gttattgtct ccatgttagt tttgggcgta gctgttatag gcttttctgc tttgcaggtt
     1501 agagctttag atgtaactgg agattcaatg tttagaactc aggcaattgc tctagcgcaa
     1561 gatttggggg agcgtattat gttaaaccca aatggagcaa caaaatataa agcaacatgg
     1621 aatgcaacta atagcagtga ttgtgaaaca gcaagttgta cgcctgaaca aatggcagaa
     1681 tatgatatta aaagtgttac caacacagct aaacaaatct tgcctaatgg tcaaataaaa
     1741 ttaattaagt gtatagaacg aaataattat tgtgtgtacg ttacttggaa taaaacacaa
     1801 cctaatgctg agggagctgc tgatagctgt tctaatgaaa atgatacttt tgtaaccaat
     1861 gctgactgcg ttaaattgga gacttcatta ttatgatgac tcaatatagc aaaggtttta
     1921 ccttaataga gttaatggtt tctttgacat tagccttgat tattatgttg tctgctatgc
     1981 aattgttatt aaccaaccaa atgagtttta acttgcaaag tgggatgggg aatgttcaag
     2041 aaaatggccg ttttgcactg gattatatta atagtacagt tcgtagtgca gagtacagcc
     2101 aaaatattgc aactccaaca acaggcatta ttatgtttaa agatgagctg ccgagtgatg
     2161 ctgccaataa aatcgaagat agtcatgtta caaataatga tattactctt atagaaacac
     2221 aagtttcacc tggtgttact aaaaataaag gctatagcga tcaattggta actcgtatgt
     2281 ggattagcca agcgatgatt gatggtggtt ttagagattg tgaggggaat gctgttacta
     2341 ctcctggttt tgtggttaca cgctactttg ttcgtgatga tctaacaagc aataaggagg
     2401 ctttggcttg tgacgcaggt ttttatcaag ccgatgcagc ttctgtcagt aactataaag
     2461 ataacggagt agttttactc tctactatag attcatttca ggttttattt ggcgtaagtg
     2521 ctttcccttt acctgcatta ggtcaacggt ttcctgtgca atatatgaca gcaaaagctt
     2581 atacagatat tccagttgca tctcgtccgg cgattgtgtc attacgtatt ggtttagtgg
     2641 ttcgctctaa tgaaaaagtt aacacgggat ttaatagtac acctgaaaaa ataactgttt
     2701 tagataaaga aatttctgga agtaatttag acgataagtt tgttcatcgt ttatttacga
     2761 gtacgttggc attgcgaaat gcgatttgag ggtaaaataa tgaataaaac acccatcaaa
     2821 accttacagc aaggcatggc tttgatatct gtattggtta ttttgttaat tgttacattg
     2881 ttaggtgtaa ctgcaatgcg tatggggctg tctggattaa tgttggcaaa taatagtcaa
     2941 gtatcacaat tgttatttca gtcagcagat atgggtacgg tgcaattaac caatactatt
     3001 aatgaaaata tattggactc tatggatgta acaggagtaa ttggtaatgc atcaggtgcg
     3061 gatactcatt tgtgtctaaa gcctactact actggaacaa attttaaata ctttaaagct
     3121 ggagcttgtg atcctactag tagtagtgat tatttgagta aaagagaagt tgttttaact
     3181 caagtaagtt atagccgtcg cacgattaca gatggtgagt ctagtaatga tttagatgat
     3241 atttctttaa ctggaagtag tatatcggtc acgggagcag cagaaaaatt gaaaactttt
     3301 tcaactgccg ttattccaag ctttggaaca gtaagtgcga gtgtaatcaa aaattgtttg
     3361 aataaagcaa atgctgatga tgaagaaagt gacaatgcat cagcagaaac aattacagat
     3421 tgtttaactg acgtgggtgc agtatttaca actcatgcaa ccgaatatcg tattcagcgc
     3481 caataacata aacaggtgag ttatgaaaaa caataaatct atcagtttca aaaaaaacta
     3541 tttggcaaca gccattgcct atgtttatgt aagtttggca gtggctccag tgtatgcgag
     3601 tgacaccgaa atttatgtgg atagtacaca atcttcggcg gtgtcgccta atttaatgat
     3661 ggtatttgat acttcgggta gtatggctga gtgtgttgac t
//
LOCUS       DAOQMB010000226         3688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12948_length_3688_cov_18.498486, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000226 DAOQMB010000000
VERSION     DAOQMB010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12948_length_3688_cov_18.498486"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="PLY05_07870"
     CDS             <1..1029
                     /locus_tag="PLY05_07870"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNC03258.1"
                     /translation="FIYAPADIENVKASHAGTNNGDMIPPTWVGSGFISHQMNDNLTV
                     GVGNYAPFGLSTNYESSFQGRFFGDKSKVKVIAIQPTIAYKINDNFSVGLGLSASKIE
                     GLLSAAVAPIPSNPSLEVMGDDISFGYNLGLLWKLDEQTRVGFNYRSETEYNLEGTTK
                     VFNFPVNGAYLNYDANLDISTPATIEVSISHQLNSDTNIHASIGLTQWSVLENLIIKN
                     QNAPSNFATITEELHWEDSWAYSVGADWKSGHGVTYRAGLGVDNTPVTDEHRSVRVPS
                     EDRTVLSLGLSYPFNDKMSIDAAYMFLKEKTAHIDRVKGAGNSGVTYSADYKGVAHLI
                     GAQLNMKF"
     regulatory      1248..1442
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1518..3320
                     /locus_tag="PLY05_07875"
     CDS             1518..3320
                     /locus_tag="PLY05_07875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC03259.1"
                     /translation="MFSRSFLAASIAACSSFVFAAEATLPTTVVTATRTTQSVDDSLA
                     SVQVITQEQLTQHPSQDLGEILRFKAGIDVARLGGFGGQTSTFIRGTESNHTLVLIDG
                     VRINSATSSQANIQHLTLDDIERVEIVKGAMSSLYGSEAIGGVINIITKTPNKTSSQA
                     NITSGSHNLIKGGFNQTVKHNQFSALINANAIYTDGFPIIEKNAQEYGYKNQGVNLKA
                     NYDLGFSQIAVSTRQNKGTTEYNNFGSFATQDFENQLISLSAKGDINDKLNSQIRLSQ
                     LKDYIDQNQSTSIAHTKQQQADWQNTFALSSEITLVAGITQTNTEAQYDNGFGTKYDK
                     EQDNLAVYLQQQSEFGQISTQLSVRHEDYDSFGTHQTGTLALGYSLNNQHRIYANYGT
                     AFKAPDLNDLYGYGGNVNLKPEESTSVEVGSKHTIGQFKLNTAFYQYDIDNLISCVGG
                     FPCQNINVDKASIKGVELGISWQQDSLMFGLNGGYNHAQDDMTKQDLLRRPRRSLSFT
                     TGYNQEKWGISSEVLAKSHALDAPLFGSNTPRRLAGYAVANMNAYWQVVPSTKLRVSL
                     ENITDKTYSFAYSGATTRYLATPFTASISADVKF"
     gene            3378..>3688
                     /locus_tag="PLY05_07880"
     CDS             3378..>3688
                     /locus_tag="PLY05_07880"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HNC03260.1"
                     /translation="MGHRLSKIYTKTGDDGTTGLGDGSRVAKDSLRVEAYGTVDELNS
                     FIGLLRAELTANHHMETILSKIQHELFDLGGELCIPNFILIKEEAISRLEQAIDSLNE
                     D"
BASE COUNT         1135 a          671 c          719 g         1163 t
ORIGIN      
        1 tttatttatg cacccgctga cattgaaaac gttaaagcat cacatgcagg aacaaataat
       61 ggcgatatga ttccacctac ttgggttggt tcgggtttta ttagtcacca aatgaatgat
      121 aatttaactg ttggtgtggg taattacgca ccatttggtt tatcaactaa ttatgaaagc
      181 tccttccaag gtcgtttttt tggcgataaa agcaaagtca aagttattgc tattcagcca
      241 acgattgctt acaaaattaa tgacaacttt tctgttggtt taggtttgag tgctagcaaa
      301 attgaaggtt tattatcggc tgcggtagct ccaatcccta gtaatcctag tcttgaagtc
      361 atgggtgatg atataagttt tggctataac ttaggcttat tatggaaact cgatgaacag
      421 actcgtgttg gttttaatta ccgaagcgaa accgaatata acttagaagg aacaacaaaa
      481 gtttttaact ttccagtcaa tggtgcatat ttaaactatg atgcaaattt agatatttcc
      541 accccagcaa ccatagaggt atctattagc catcagctca atagtgatac taatattcat
      601 gcaagtattg gcttaactca atggagtgtt ttagaaaact taatcattaa aaatcaaaat
      661 gcaccatcta attttgccac tattacagag gagttacatt gggaagactc atgggcatat
      721 agtgttggtg cagactggaa aagtggtcat ggcgtcactt atcgtgcagg tttaggtgtc
      781 gataatacac cagttactga cgagcatcgc agtgtacgtg tgcctagtga agatagaacc
      841 gtattaagtt taggattaag ttatccattc aatgataaaa tgtctattga tgcggcctac
      901 atgtttttaa aagagaaaac agctcacatt gatcgcgtga aaggcgcagg caatagtggt
      961 gtaacatata gcgcagatta taagggtgtt gctcatttaa ttggcgcaca gttaaatatg
     1021 aagttttaaa cgctaaaacc tccagtcaaa attgtgctat tcggacaaac taaaaattaa
     1081 atttagtttg tccaaccttt gtcttgcatc tctcacacat cttacctaat atccgccctg
     1141 ctttatggtg tccgtttaag tacggtttga ctttttagga cttacgcggt tatcgcttat
     1201 ttatgcatat ttcaggcatt tttaatgccc ttatgttcat aaaacgcgct aatttcaacg
     1261 aaatgcgtaa accctatcat gttacaaatg ggaaactggt gcaagtccag tgctgccccc
     1321 gcaacggtaa ctatgtttgt ttgttttata tgccactgtt tttaaatggg aaggctaaat
     1381 aaacggctgt cttagacagt cagcatagga gtccggagac cgaccatagt gcctatttaa
     1441 cgctcttttt ttggacaagt ggcgttttct tttcattttt tcgctgtttg cggggaacca
     1501 aacagtcgga gttatttatg ttttctcgtt catttttagc agctagtatt gctgcctgct
     1561 cgtcttttgt ttttgctgct gaggctacgt tgccaacaac cgtcgtcact gcaactcgta
     1621 ccacacaatc tgttgatgat tctttagcct ctgttcaggt gattactcaa gaacagttaa
     1681 ctcagcaccc ctctcaagat ttgggtgaaa tattaagatt taaagcaggt attgatgttg
     1741 cacgtttagg tggttttggc ggacaaactt ccacttttat tcgtggtaca gaaagtaacc
     1801 ataccttagt gctaattgat ggtgtacgta ttaactcagc cactagcagt caagccaata
     1861 ttcaacattt aactttagat gatattgaac gcgttgaaat tgttaaaggg gcaatgtcta
     1921 gcctatatgg ttctgaggcc attggtggtg tgattaatat tattactaaa acaccaaaca
     1981 aaacttctag ccaagccaat attacgtctg gtagtcataa cttgattaaa ggtggtttta
     2041 accaaacagt taagcacaat cagttttctg cgttaattaa tgctaatgct atttataccg
     2101 atggttttcc gattatcgaa aaaaacgctc aggaatacgg ctataaaaat cagggcgtta
     2161 atttaaaagc caattatgat ttaggcttta gtcaaattgc tgtcagcaca cgacaaaata
     2221 aaggtactac cgaatataat aattttggct catttgccac tcaagacttt gaaaatcaac
     2281 tcattagctt gagtgctaaa ggcgatatta atgacaaatt aaacagtcaa attcgtttaa
     2341 gtcagctcaa agattatatt gatcaaaacc aaagtacctc tattgcacat accaagcaac
     2401 aacaagccga ttggcaaaat acctttgcac tttcctcaga aatcactttg gtagcaggca
     2461 tcacccaaac caatactgaa gcacaatatg acaacggttt tggtactaaa tacgataaag
     2521 agcaagataa cttagctgtt tatctacaac agcaatcaga gtttggtcaa attagcaccc
     2581 aactttctgt tcgtcatgaa gactatgatt cgtttggcac acatcaaaca ggtactttag
     2641 ctttaggcta tagcctcaat aatcaacacc gtatttatgc caattatggg acagcattta
     2701 aagcacctga tttaaacgat ctatatggtt atggtggtaa tgttaattta aaacccgaag
     2761 aatcaacttc tgtagaggtg ggtagtaaac atacaatcgg ccaattcaag ctaaatacgg
     2821 ctttttatca atatgatatt gataatttaa ttagttgtgt gggtggtttt ccttgccaaa
     2881 atattaatgt tgataaagca tcaattaagg gtgtagagct tggcattagt tggcaacaag
     2941 atagtttaat gtttggtttg aacggtggtt ataatcatgc ccaagatgat atgactaaac
     3001 aagatttatt acgtcgtccg cgtcgtagct taagctttac tacaggctac aaccaagaaa
     3061 aatggggtat ttcaagcgaa gtattggcta aaagtcatgc gttagatgca ccattatttg
     3121 gcagtaacac accacgtcgt ttagctggct atgctgtcgc taatatgaat gcttattggc
     3181 aagttgtacc aagcactaag ttacgtgtgt cgctcgaaaa tatcaccgac aaaacctata
     3241 gttttgctta ttctggggca acaacacgtt acctcgccac tccattcact gcttcaatca
     3301 gtgcagatgt aaaattctaa tccaattgat tatcggggta gcctttatgg ttacccccat
     3361 tattaaagag ctatgttatg ggacatcgtt tatcaaaaat ttataccaaa acaggtgatg
     3421 acggcacaac aggtttaggt gatggtagcc gtgtggctaa agatagttta cgtgttgaag
     3481 cctatggcac agtggatgaa ctcaatagtt ttataggttt attacgcgca gaattaacag
     3541 ccaatcatca catggaaact attttatcta aaattcaaca tgaattattt gatttgggtg
     3601 gtgagttatg tattcccaat tttattttaa tcaaagaaga agcaatctca cgtttagagc
     3661 aagcgattga ctctttaaat gaggattt
//
LOCUS       DAOQMB010000227         3684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_12971_length_3684_cov_5.662993, whole genome shotgun sequence.
ACCESSION   DAOQMB010000227 DAOQMB010000000
VERSION     DAOQMB010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12971_length_3684_cov_5.662993"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2627)
                     /locus_tag="PLY05_07885"
     CDS             complement(<1..2627)
                     /locus_tag="PLY05_07885"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC03261.1"
                     /translation="MMTEDQLEQEMLGYLADIGYQCINGLDIAPDSSQPERSNYQQVI
                     LTERLRQAIHRLNPQIPAFAREQALSQVLNLDSPIQLVANRRFHHYLVNGVAVEYQKD
                     GETRGDFVRLADFENQKNDWLAVNQFTIKGAKHTRRPDVLLFLNGLPLVVIELKNPAD
                     ENADIWKAYEQLQTYKEQISDLFQYNEILVISDGTEARMGSLSSNAERFLAWRTIDGN
                     TLDPLGQFNELETLTRGILTPNLLLDYLRFFVLFEDDGQLIKKIAGYHQFHAVRSAIN
                     QVIAASQPQGNQKGGVVWHTQGSGKSITMTCFAARVMRETAMHNPTIVVITDRNDLDG
                     QLFGVFSLAQDILREQPVQAESRQDLRDKLSSRPAGGIVFATIQKFMLADEETVFPVL
                     SDRHNIVVIADEAHRTQYGFNAKLKVKRTRPTTAANDAYANAEPQTLMAAEPKADYQA
                     SYQVGYAQHLRDALPNATFVAFTGTPVSSEDRDTRAVFGDYIHIYDMQQAKEDGATVA
                     IYYESRLAKLGLKESELPQIDEEVDELAEDEEEAEQSRLKSRWAALESIVGAPPRIAQ
                     VAADLVSHFEERNQAQQGKAMIVAMSREICVHLYNEIVKLRPDWYNEDPEKGAIKIVM
                     TCSASDKALLRPHIYSHIVKKRLEKRFKDPHDPLKLVIVRDMWLTGFDAPCVHTLYVD
                     KPMKGHNLMQAIARVNRVFKDKQGGLVVDYIGIANELKSAIKEYTNSKGRGRPTVDAH
                     EALAVLLEKMDIIRGLLHGFDYSGFLTGGHKTLAQTANYVLGLEDGKKRFADNTLALS
                     KAFSLCCTLDEAKALREEIAFFQAVKVLLTKRDTSQKKLSNEERELAIRQIIGQAIVS
                     EDVVDIFNAVGLEKPNIGL"
     gene            complement(2662..3063)
                     /locus_tag="PLY05_07890"
     CDS             complement(2662..3063)
                     /locus_tag="PLY05_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03262.1"
                     /translation="MNGYLLDTNACIAILKGNPTILANIRRVGVRQLYLCAPVKAELW
                     FGACKSAKTVENKQKLETFFAELPSLAFDDKVIKTQAEVRMALFAKGTPIGPYDMQIA
                     AIALSYDLTVVTHNVREFERVPDLLIEDWLV"
     gene            complement(3063..3305)
                     /locus_tag="PLY05_07895"
     CDS             complement(3063..3305)
                     /locus_tag="PLY05_07895"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNC03263.1"
                     /translation="MNIAQTLQTHILALPTSFQVEVLDFVEYLEKKYGVGLAAKANDN
                     EQTEAFIARMTHSLGDDFPDDINDVDLGEDSVKDFL"
BASE COUNT         1002 a          802 c          707 g         1173 t
ORIGIN      
        1 aacaagccga tattgggctt ttccaaacct accgcattaa aaatatcaac tacatcttcc
       61 gagacaatcg cttgaccaat aatttgtcga atggctaatt cacgctcttc gttgctcaat
      121 ttcttttggc tggtgtcacg cttggtgagc aacactttaa ccgcttgaaa aaaagcaatc
      181 tcttcgcgca gggctttagc ttcatctagg gtgcaacaca aactaaaggc tttacttaac
      241 gccagtgtat tgtcggcaaa gcgttttttg ccatcttcta agcccaaaac atagttagcc
      301 gtttgcgcta aggttttatg gccacctgtt aaaaagccac tgtaatcaaa accatgcaac
      361 aagccacgaa taatatccat tttttctaat aacacggcta aggcttcgtg ggcatcaacg
      421 gtgggtcggc cacgtccttt gctgttggtg tattccttaa tggcactttt taattcattg
      481 gcaataccaa tatagtccac caccaaaccg ccttgtttgt ctttgaatac ccgatttaca
      541 cgggctatgg cttgcattag gttgtggcct ttcatgggct tatcgacata cagggtatgc
      601 acacacggtg catcaaaccc tgttagccac atatcacgca caatcaccaa ctttaagggg
      661 tcatgtggat ctttaaagcg tttttcgagg cgttttttga caatatggga atagatatga
      721 ggccgtaata aggctttatc gctggcactg catgtcatca ctattttaat tgcgcctttt
      781 tcggggtctt cgttgtacca atcgggacgt agcttgacga tttcgttata cagatgaacg
      841 cagatttcgc ggctcatggc aacaatcatc gctttgcctt gttgtgcttg attgcgttct
      901 tcaaaatgag acactaaatc tgctgcgact tgagcaatac gcggtggtgc gccgacaatg
      961 ctttctaacg ctgcccaacg gcttttgagg cgtgattgtt cggcttcttc ttcatcttcg
     1021 gctaattcat ccacttcttc gtcaatctgt ggtaattcgc tttcttttag ccctaatttt
     1081 gccaaacgcg attcgtaata aatggccacc gttgcgccat cttctttggc ttgctgcata
     1141 tcgtagatat gaatgtaatc accaaatact gcacgagtgt cacggtcttc gctagagaca
     1201 ggcgtacctg taaacgcaac aaaagtggca ttcggcaaag catctcgcag atgttgagcg
     1261 taacccactt gataactggc ttgataatca gcctttggct ctgccgccat taacgtttga
     1321 ggttctgcat tggcataagc atcgttagcg gcagttgttg ggcgagtgcg tttgactttg
     1381 agtttggcat taaaaccata ttgggtgcga tgcgcttcat cagcaataac cacaatattg
     1441 tgacggtctg acaagacagg aaatactgtt tcttcatcgg cgagcataaa cttttgaatg
     1501 gtggcaaaga caataccgcc cgcaggccgt gatgatagtt tgtcacgcaa atcttggcgt
     1561 gattcggctt gcacaggttg ttcgcgcaaa atatcttgtg ctaatgaaaa cacaccaaat
     1621 aactgaccat ctaagtcgtt acggtcggtt atgaccacaa tcgttgggtt gtgcatggct
     1681 gtttcgcgca tcactcgtgc cgcaaaacag gtcatggtaa tactttttcc gctaccttgc
     1741 gtatgccaaa ccaccccgcc cttttgatta ccttgtggtt gtgaagctgc aatcacttga
     1801 ttaatggcag agcgaaccgc atgaaattga tgataccctg caattttttt aatcagttgg
     1861 ccgtcatctt caaacaacac aaaaaagcgc aaataatcca gcaataaatt gggcgttaaa
     1921 ataccgcgtg ttagcgtttc tagttcgtta aattggccaa gtgggtctaa ggtgttgccg
     1981 tctatggtac gccatgccaa aaagcgttca gcattgctcg ataacgaccc cattcgtgct
     2041 tctgtgccgt cactaatcac taaaatttcg ttgtattgaa ataaatcgct gatttgctct
     2101 ttgtaggttt gtagttgctc gtaggctttc caaatatcgg cattttcatc ggcaggattt
     2161 tttaattcta tgactaccag tggtaagcca tttaaaaaca atagcacatc aggacggcgt
     2221 gtatgttttg cgcctttaat ggtaaattgg ttgaccgcta accagtcatt tttttgattt
     2281 tcaaagtcgg ctaaacggac aaaatcacca cgcgtttcac cgtctttttg atattcaacc
     2341 gccacaccat tgactaaata atgatgaaaa cggcgattag ccaccaattg tatggggcta
     2401 tccaaattta gcacttggct tagggcttgc tcacgggcaa aagcaggaat ttgtggatta
     2461 agccgatgga tagcttggcg taggcgttcg gttaaaatga cttgttggta gttgctacgt
     2521 tcgggttgtg agctgtctgg agcgatgtct aaaccattaa tacactgata acctatgtct
     2581 gctaaatagc ctaacatttc ttgttctaat tggtcttcgg tcatcatctg agagtagtcc
     2641 ttttttatat gtttattatc gctaaaccaa ccaatcttct atcagcaaat caggtacacg
     2701 ttcaaactcg cgcacattat gtgtcactac tgttaaatca taactcaagg caatagctgc
     2761 tatttgcata tcatatgggc caattggtgt tcctttagca aacaaagcca tacgtacctc
     2821 agcctgtgtt ttaatcactt tatcatcaaa agctaaactg ggtaattctg caaaaaaagt
     2881 ctctaatttt tgtttgttct caactgtttt agcactttta cacgctccaa accataactc
     2941 cgctttaaca ggtgcacata aatataattg gcgtactcct actcggcgaa tattagccaa
     3001 gatcgtagga ttgcctttta atatggcaat acacgcattg gtatctaaca aatatccatt
     3061 cattacaaaa agtcctttac actatcttca cctaagtcca catcattaat atcatcagga
     3121 aaatcatcac ccaagctatg cgtcatacgc gctataaaag cttctgtttg ctcattatcg
     3181 ttggctttag cagctaatcc tacgccatat tttttttcta aatactcaac aaaatccaaa
     3241 acttccacct gaaaactggt tggtaaagcc aaaatatgtg tttgaagtgt ttgtgcaata
     3301 ttcattttta agcccttaac aggttaaata atcttagatt ctatcatcaa aaatgatacc
     3361 ttaatgtaat atagcttgac acgagatact cttgttctaa ttggtcttcg gtcatcattg
     3421 atgagtagtc cttttaaatt tactgctatt acaagcactt gctttaagct tagttgttat
     3481 ttcaacgctc taacatttgt taatatacta acaatttagt catcaccttg ctaaataatt
     3541 gttcatctta aataaatttt ttgttaatca tatagttata atcttattaa ggacgttttc
     3601 tcttgtatat gactacgttt tccttgctat taatttgata aaaaaacttt ttaaatcatt
     3661 agcttaatat ttattaaggg caaa
//
LOCUS       DAOQMB010000228         3680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13000_length_3680_cov_6.409655, whole genome shotgun sequence.
ACCESSION   DAOQMB010000228 DAOQMB010000000
VERSION     DAOQMB010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13000_length_3680_cov_6.409655"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="PLY05_07900"
     CDS             complement(<1..181)
                     /locus_tag="PLY05_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03264.1"
                     /translation="MILKKHCLATAVACAYMTLAIAPAYASDTEIYVDNIQSSAIAPN
                     LMMVFDTSGSMAECVD"
     gene            complement(228..914)
                     /locus_tag="PLY05_07905"
     CDS             complement(228..914)
                     /locus_tag="PLY05_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03265.1"
                     /translation="MNNKSSSAKKLQQGAALIAVLVVLLIVTVLGVTAMRMGLSGLVL
                     ANNSQVSQLLFQSADMGTVQIRKTIEMDVKGALQLTGVVGNPTGGDTHLCFRPRDNGT
                     DFKHFKTGACDPSDTASYLSQRAIVLTQVSYNRNVITDGDSSNDVDDISREGSSTSAT
                     GASERLRTFSTSVVPSFGSASQTTIKECLQQPADDEDVQNSSVYTITDCLTKVGAVFT
                     THVTEYRIIK"
     gene            complement(926..1783)
                     /locus_tag="PLY05_07910"
     CDS             complement(926..1783)
                     /locus_tag="PLY05_07910"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HNC03266.1"
                     /translation="MMTQHSKGFTLIELMVSLTLALVIMLSAMQLLLTNQMSFNLQSG
                     MGNVQENGRFALDYINRMVRSAEYSQNTANPTIGIITNQAELPGLINNDLVTRNDLTN
                     LGKGLSDQLVTRMWVSQAMIDGGFRDCEGNAITTPSFVVTRYFVRADSVTGSDAALAC
                     DAGNYAVDAAAVASYGDDGVVLLSTIDSFQVLLGVSAFPVPALGQRFPVRYMTAKIYD
                     DTPVASRPMIVSLRMGLLVRSNEKVNTGFNDEPKPIQVLDKLILGSALNDKFIHRVFT
                     STLALRNAI"
     gene            complement(1780..2286)
                     /gene="pilV"
                     /locus_tag="PLY05_07915"
     CDS             complement(1780..2286)
                     /gene="pilV"
                     /locus_tag="PLY05_07915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HNC03267.1"
                     /translation="MFNQSCALNKQQGFGMTEIIVSMLVLGVAVIGFAALQIRALDTT
                     GEAMFRTQAMALAQDLGERIILNPDGATTYKNWTTGSTVNCETGNCTPADMAKYDITG
                     VIKLAQDTLPNGQITVLKCISQNNYCVYVSWNKTKPNADAAADSCSQTDDRYVPGADC
                     VKLETSLL"
     gene            complement(2296..2826)
                     /locus_tag="PLY05_07920"
     CDS             complement(2296..2826)
                     /locus_tag="PLY05_07920"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HNC03268.1"
                     /translation="MSKQRARGFTIIELMVSVAVIATILAFAFPSMRLMQTNSRMASA
                     ANDLATDLKQGRAAAVTNRTNFTLAAQVGGWSNGWQATFMASTDAAENSVQTQRMALQ
                     KIGLPPGTTIVQSVNPLIFNGVTGRVTDNAGNSVDVVFRICNSQISNETGFDVMLNRF
                     GRILIQRHTNSTVCGS"
     gene            complement(3096..>3680)
                     /gene="ispH"
                     /locus_tag="PLY05_07925"
                     /gene_synonym="lytB"
     CDS             complement(3096..>3680)
                     /gene="ispH"
                     /locus_tag="PLY05_07925"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394089.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNC03269.1"
                     /translation="GHPEVEGTMGQYDTQFGGQIYLVEDEQDVAKLIVKNPQQLAFVT
                     QTTLSVDDTARVIDALRAKFPAIQGPRKDDICYATQNRQDAVKTLAQSCDIVLVVGSP
                     NSSNSNRLRELAERMGAKAYLIDNAEQLQKEWFDSTQKIGLTAGASAPEILIQQVVQR
                     LQQWGGQTPQEITGREENVVFSMPKELRIPSIAL"
BASE COUNT         1120 a          820 c          598 g         1142 t
ORIGIN      
        1 agtcaacaca ctcagccata ctacccgaag tatcaaatac catcattaaa ttaggtgcaa
       61 tggctgatga ttgtatgtta tccacataaa tttctgtgtc actggcataa gcaggcgcaa
      121 ttgctaaggt catataagca caagcaacag ctgttgctaa gcaatgtttt tttaatatca
      181 tagggttact gctcttcata acacacctgt ttaatttttg ttaggcctta tttgataata
      241 cgatattcag tcacatgtgt tgtgaatacc gcacctactt tcgtcaaaca atcagtaatt
      301 gtgtaaaccg atgaattttg tacatcttca tcatcagctg gttgttgcaa acattctttg
      361 atagtcgtct gactagctga accaaaacta ggtactacag atgttgaaaa cgttcttaat
      421 ctttcactag ctccagttgc ggaagtacta ctcccttctc gtgaaatatc atcaacatca
      481 ttactagagt ctccatcagt tatcacattt cgattataag agacctgcgt caacactatt
      541 gccctttgac tcaaataact tgcagtatca cttggatcac aagctccagt tttaaaatgc
      601 ttaaaatcag taccattatc tctaggtctg aaacacaaat gcgtatcacc acccgtcgga
      661 ttacctacca cacctgttaa ctgtaaagct ccttttacat ccatttctat tgttttgcgt
      721 atttgtactg tccccatatc ggcagattga aataataatt gagatacctg actattattg
      781 gccaatacta atcctgacaa ccccatccgc attgcggtta cacctaaaac agtcactatt
      841 aacaatacaa ctaatacagc tattaacgct gcaccttgct gcaacttttt agcagatgat
      901 gacttattat tcattttatc gcacctcaaa tagcattacg caacgctaaa gtactggtaa
      961 acactcgatg tataaattta tcgtttaaag cactacctaa aataagttta tcaagaactt
     1021 gtatcggctt aggctcatca ttaaatcctg tattaacttt ttcattggag cgaaccaaca
     1081 agcccattcg taatgacaca atcattggac gtgatgcaac gggagtatcg tcataaattt
     1141 tggctgtcat atatcgaact gggaaacgct gtcccaacgc tggaacagga aaagcactta
     1201 caccgagcaa aacttgaaaa ctatcaattg ttgacaatag aactactcca tcatcaccat
     1261 aagatgcgac tgctgctgca tctacagcgt aattaccagc gtcacatgcc aaggcagcat
     1321 cactacctgt tacagagtct gcacgaacaa agtagcgtgt cactacaaaa ctaggcgtag
     1381 taatcgcgtt accttcacaa tccctaaaac caccatcaat catggcttga ctaacccaca
     1441 tacgagtaac caattgatca ctcaagccct tacccagatt agtcagatcg tttctagtta
     1501 ctaagtcatt atttatcaaa ccaggaagtt ctgcttgatt ggtaataatt ccgatggtcg
     1561 ggttagctgt attttgacta tattctgcac tacgcaccat tctattaata taatccaacg
     1621 caaagcgacc attttcttgc acattcccca taccactttg caaattgaaa ctcatttgat
     1681 tggttaataa caattgcata gccgataaca tgatgactaa agccaatgtt aacgaaacca
     1741 ttaattctat caaggtaaaa cctttactgt gttgagtcat cataataatg aagtctccaa
     1801 tttaacgcag tctgctccag gaacatatct atcatctgtt tgtgaacaac tgtcagcggc
     1861 ggcatcggca ttcggttttg ttttattcca tgagacataa acacaataat tattttgact
     1921 aatacactta agcactgtta tttgaccatt aggcaatgta tcttgtgcta attttattac
     1981 accagtaata tcatattttg ccatatcagc aggagtacaa tttcctgttt cacaattaac
     2041 tgtactacct gtagtccaat ttttgtacgt tgtcgcacca tcaggattta atataattcg
     2101 ctcacccaaa tcttgagcca aagccattgc ttgtgttcta aacattgcct cacctgttgt
     2161 atcaagagca cgaatctgca aggcagcaaa gcctataact gcaaccccca aaaccaacat
     2221 agacacaata atctcggtca tgccaaatcc ttgttgtttg tttaatgcac atgattgatt
     2281 aaacatttta atctcctaac taccacaaac agtgctatta gtatgacgct gaatcaatat
     2341 acggccaaaa cgattcaaca taacatcaaa accagtttca ttagaaattt gactgttaca
     2401 aattctaaaa accacatcaa ctgaattacc tgcgttatct gtaactcttc ccgttacgcc
     2461 attaaaaatt aacggattga ctgattgaac aatcgttgta cctggtggta atcctatttt
     2521 ttgcaaagcc attctttgtg tttgtactga gttttctgct gcatcggtac tcgccataaa
     2581 cgttgcttgc caaccatttg accacccccc cacttgcgca gcaagagtaa agttggtacg
     2641 gttagttact gcagcagcac gtccttgttt taaatctgtt gctagatcat ttgcagctga
     2701 ggccatccgt gaattagttt gcattaatcg catagatgga aaagcaaatg ccaaaatagt
     2761 agcaattaca gcaactgaaa ccatcagctc tattatggta aatcctcttg ctcgttgttt
     2821 agacataaaa cacaccccgt aacacttaaa ttagaaccga tcttatattt attaaagccc
     2881 tatttagtgt ctaatttaac agtacctgac acagtaccga ctttgttaag tgaacattag
     2941 acattaaata accactcctc aagcagaacg cgacagtcgg ctatttttaa ttgaccaaga
     3001 ggtcattttt aatattttgt tacatttttt gttagacaat aggcgtcaaa gagtgacaat
     3061 aaatgtcacc ctttggatat gaatttaaat tgtttttata atgcaataga aggaattcgt
     3121 agctctttag gcattgaaaa gaccacattt tcttcccttc ctgtgatttc ttgtggggtc
     3181 tgaccgcccc attgttgtaa acgttgcaca acttgttgaa tcaatatttc aggcgctgaa
     3241 gcacctgctg ttaaaccgat tttttgtgtt gaatcaaacc attctttttg taattgttct
     3301 gcattatcaa ttaaatatgc cttagctccc atacgctcag caagttcacg cagtcggttt
     3361 gagttagagg agttaggaga accaacaacc aatactatat cacaactttg tgctagagtt
     3421 ttaactgcat cttgacgatt ttgtgtcgca taacaaatat catctttacg cgggccttga
     3481 atagctggga acttggcgcg tagggcatca atcactcgtg cggtgtcatc taccgataac
     3541 gttgtttgtg tgacaaaagc taactgttgt ggatttttca caattaactt cgcaacatct
     3601 tgttcatctt caaccaaata aatttgacca ccaaattgag tgtcgtactg ccccatggtg
     3661 ccttccactt cgggatgtcc
//
LOCUS       DAOQMB010000229         3658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13116_length_3658_cov_12.854566, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000229 DAOQMB010000000
VERSION     DAOQMB010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13116_length_3658_cov_12.854566"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            219..1208
                     /locus_tag="PLY05_07930"
     CDS             219..1208
                     /locus_tag="PLY05_07930"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNC03270.1"
                     /translation="MSAPTLNQYLVQAKVPAQLQQLITQVSVACKAISVAIGKGALAG
                     ILGQAGSDNIQGEEQKKLDVISNDIMLAAGLGSGSIAGLASEEMDDIFPLPDGTPLGD
                     YLMLFDPLDGSSNIDVNVSVGTIFSILKKPLVAQSVITTEDFLQAGSQQVCAGYAVYG
                     PSTLLVLTLGNGVDCFTLDREIGEFVLTQTNMTIPADTKEFAINMSNMRFWEQPVRRY
                     VDEMVAGKTSVRGKDFNMRWVASMVADVHRILTRGGIFMYPYDVKDPSKAGKLRLMYE
                     ANPMSFIVEQAGGASTTGRERILDLKPTGLHQRVPVILGSKNEVELVTGYHHE"
     gene            complement(1292..3334)
                     /locus_tag="PLY05_07935"
     CDS             complement(1292..3334)
                     /locus_tag="PLY05_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03271.1"
                     /translation="MAHTWRFFRSSGFDQVVIDSVADLENLGELDKKLWAALACPTKG
                     LELDARTLAYIDADGDGRIRVPELLAAIDWTLVHLSQKEVLFQTAGLSLAAIDGQHAD
                     GAKILAAAQQLLQRLGKATATTINADDTQDLGKMFPANQVNGDGLIPAALAVEQELAD
                     AINDMIATLGGEVDRSGEQAVSADKIKQFFADIRTMSEWESRENSTISLFGQQTAAAY
                     DAFSAVTAKIDDYFVRVQLAEFDDKATVALNASDAQYAALAGQLLAVNNSDTQSLPLA
                     KIAANQALNLTQGINPAWQAAIDNFSQLVLANNSQELTLAQWTQIKADFAAYSAWLQE
                     KPALAVVNLDATKRQMLANSLIEQALLALVEDDLKVADAANALVDLDKLVRYQANLVT
                     LVNNFVSFSDFYSRKEKAIFQAGTLFIDGRACDLAVRVNDMAKHGSMAGLSNTYLVYC
                     DCTRKDSPEKMTIVAAVTAGDVGNLMVGRNGVFYDRNGKDWDATVVKLIENAISVKEA
                     FWTPYRRLGRMVSNQLQKMASEQDKAIEAKTSENVTAGIGKVQAAAKTPADAAKPATP
                     PAPFDVAKFAGIFAAMGLAVGAIGTAIASVVSGFMALIWWQMPLAIIGLLLMISGPSM
                     IMAWFKLRQRNLSPILDANGWAVNTNAKVNIAFGESLTILATLPKGAQRSLRDPFA"
BASE COUNT         1081 a          808 c          661 g         1108 t
ORIGIN      
        1 tcaccgcctt atatcctcgg cctaaaggcg cgaggtttta cggcgatttt gataatgaat
       61 gtgaagaaca accaaacgtg aaaacgtgaa atatatcaca ttttagtggt tgaccaagac
      121 ggatttacca tagtttttaa aacacctagc atcaagttca tatctaagcg ataatttagt
      181 tattatcccc tcatctcgat caacacaatg gagagattat gtccgcgcct acccttaatc
      241 aatatttagt gcaagccaaa gtgcctgccc aattacaaca gttaatcacc caagtgagtg
      301 tggcttgtaa agcaattagt gttgctattg gtaaaggcgc attagcaggt attttaggac
      361 aagcaggttc tgacaacatt caaggtgaag aacaaaagaa gctagatgtc atttctaatg
      421 acattatgct tgccgcaggt ttaggttcag gcagtattgc tggcttagcc tccgaagaaa
      481 tggatgatat ttttccttta cctgatggca caccattggg cgactattta atgttatttg
      541 atcctttaga tggttcatcc aatattgatg tcaatgtttc ggtggggact attttttcta
      601 ttttgaaaaa gcctttagta gcacaaagcg tgattactac cgaagacttt ttacaagcag
      661 gtagccaaca agtttgcgca ggttatgctg tctatggccc ttctacacta ttagtgttaa
      721 ctttgggtaa tggtgtagat tgttttactt tagatcgtga aattggtgag tttgtcttaa
      781 ctcaaacaaa tatgaccatt cctgctgata ccaaagaatt tgccattaat atgtctaata
      841 tgcgtttttg ggagcaaccc gttcgccgtt atgttgacga aatggtagca ggcaaaacta
      901 gtgtacgtgg caaagatttt aatatgcgct gggttgcttc tatggtagcc gatgttcatc
      961 gcatcttaac gcgtggtggc atcttcatgt atccctacga tgtaaaagac ccaagcaaag
     1021 caggcaaatt acgcttaatg tatgaagcta atccgatgag ttttattgtt gaacaagcag
     1081 gcggtgcgtc tacgacaggt cgtgagcgta ttttagacct caaaccaact ggtttacatc
     1141 aacgtgtgcc tgtgatttta ggttctaaaa acgaagtgga attagtgaca ggttatcatc
     1201 acgagtaacc actaggcttc gactccgctc agccaacacg actccgctca gcccacgcgg
     1261 agtcgttcct tgagcgaagt cgaaaggcga cttaagcaaa tggatctctt aaactccgtt
     1321 gtgcgccttt aggtaaggtc gctaaaatag tcaaactttc accaaaggca atgtttactt
     1381 tagcattagt atttactgcc caaccattgg catccaaaat cggcgataag ttacgttgac
     1441 gtaatttaaa ccatgccata atcattgatg gtcccgaaat catcaacaat aatccaataa
     1501 ttgccaatgg catttgccac catatcaacg ccataaatcc agaaactaca gacgcaatgg
     1561 ctgtaccaat tgccccaact gccaagccca ttgctgcaaa aatacctgca aatttagcca
     1621 catcaaaggg ggctggcggt gtcgctggtt ttgcagcatc tgctggcgtt ttggctgctg
     1681 cttgcacctt gccaatccct gcggtcacat tttctgatgt tttagcttca atcgctttgt
     1741 cttgctctga tgccattttt tgtagttgat tgctcaccat acgccccaaa cgacgataag
     1801 gtgtccaaaa agcctctttc acactaatcg cattttcaat taacttgaca acggttgcat
     1861 cccaatcttt accgttacga tcataaaata caccattacg cccaaccatc aaattaccaa
     1921 catcacctgc ggtaactgct gcaacaatgg tcattttttc ggggctgtct ttacgtgtac
     1981 aatcacaata aaccaaataa gtgttactta gtcctgccat agaaccatgt ttagccatat
     2041 cattaacacg cactgccaaa tcacaagcac gaccatctat aaacaatgtt cctgcctgaa
     2101 aaatagcttt ttctttacga ctataaaaat cactaaatga gacaaaatta ttgactaaag
     2161 tcactaagtt ggcttgataa cgcactaatt tatccaaatc gactaatgca ttggcggcat
     2221 cagcaacctt caaatcgtcc tcaactaatg ctaataaagc ttgttcaatc aaactattag
     2281 ctaacatttg tcgtttagtg gcatccaaat taacaactgc taaggctggt ttttcttgca
     2341 accacgcact ataagcagca aagtcagctt tgatttgtgt ccattgtgct aatgttagtt
     2401 cttgactgtt atttgctaaa accaactgac taaaattatc aatggctgct tgccatgcag
     2461 gattaatccc ttgtgttaaa tttaacgctt gattggcagc aattttagct aatggcaaac
     2521 tttgtgtatc agaattattt accgctaata attgacctgc taatgcagca tattgtgcat
     2581 ccgaggcatt taacgccacg gttgctttat catcaaattc agctaattgt acacgaacaa
     2641 aataatcatc aatctttgct gttactgcgg aaaaagcgtc ataagcagcc gctgtttgtt
     2701 gtccaaataa ggaaattgtt gaattttctc gactttccca ttcgctcatg gtgcgaatat
     2761 cggcaaaaaa ctgtttaatt ttatcggcag atactgcttg ctcaccacta cggtcaactt
     2821 caccacctaa agtcgcaatc atatcattga tggcgtctgc caactcttgc tcaaccgcta
     2881 aagctgctgg aattaaacca tcaccattga cttgatttgc aggaaacatt tttcctaaat
     2941 cttgagtatc atctgcgtta atcgttgttg ccgttgcttt acccaaacgc tgtaataatt
     3001 gttgtgcagc ggctaaaatc tttgcaccat cggcatgttg accatcaatc gccgctaagc
     3061 ttaaacccgc tgtttgaaac aatacttctt tttgactcaa atggactaat gtccaatcaa
     3121 ttgcggctaa taattctggc acgcgaatac ggccatcacc atcagcatca atataagcta
     3181 aagtgcgagc atctaactcc aaacctttag ttggacaagc taaagctgcc cataattttt
     3241 tatcaagctc acccagattt tctaaatcgg ccaccgaatc aataaccact tgatcgaaac
     3301 cactactacg aaaaaaacgc cacgtatgag ccatattact ctcactaaag ttataagaaa
     3361 aaagcaaaac taagaggact tttataacca atccttcttt tattaggata attgttaatc
     3421 ggccaactat ggccttttgt ctatttcatc acaattttat gaataattta ttctaaaaag
     3481 gtcactattt catctctttt tgtagaaatg cgattaaata ctcgatgatt ttttgtctgt
     3541 ttggatcact ttcattgact aattgatgcc tagcctctgg caaccacaat acctgctcta
     3601 ctgcaaaacg ttgtttaata aaatgaatat tatactcgcc attgaccgta ccatcttg
//
LOCUS       DAOQMB010000230         3650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13170_length_3650_cov_15.310431, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000230 DAOQMB010000000
VERSION     DAOQMB010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13170_length_3650_cov_15.310431"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="PLY05_07940"
     CDS             <1..1137
                     /locus_tag="PLY05_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC03272.1"
                     /translation="NAPINALSVSARSEGIFVQAADSASFWSLHTEHPDISMKSAWGK
                     VWYESYPEPSYTWQSTSGNADFEGKMSLMPLTFGTLKAAFYAMLLAAPLAILGAMYTA
                     VFMSPALRTKVKPAIELMQALPTVILGFLAGLWLAPTLEANLPGIVSLLIITPIIILV
                     CGFIWVQIPLEKRNRIPDGWAPVILIAPILLGGWISFASSQTIELQFFGGDIRHWLTY
                     EAGITFDQRNSLIVGLAMGFAVIAPIFSIAEDALFAVPRHLTNGSLALGATQWQTLTR
                     VILPTASPGIFSAVMIGFGRAVGETMIVLMATGNTAVMEWNIFEGMRTLSANVAVEMG
                     EAEVGSTHFRILFLSALVLFVLTFILNTVAEVVRSRLRKKYGSL"
     gene            1148..2803
                     /gene="pstA"
                     /locus_tag="PLY05_07945"
     CDS             1148..2803
                     /gene="pstA"
                     /locus_tag="PLY05_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNC03273.1"
                     /translation="MKLREYFRSGEAWVWLNAGAVAISLIMVIGILGLIAVRGLGHFW
                     PSDVMQAQYTYQGQTETIIGELYDEKVDTAKRVREAGIEIPENVSTVTRYSIKQGNKD
                     VTGQDFRWVLASNLQKQQYPADIVALERYEWGAFYGRLKAVKENGVVIADGDTAWQQL
                     QTRLQRTNDFHDQIADIEKGEIGRINFQLEKLRLQERKLELDKQLTDAARADLQAKRD
                     EQNKKYELLKAKLEQLNRDMERDTAIFVVANGTEKEIALSKIVAANKPNDMSVFAKLA
                     AYGHNAWAFLSDDPREANTEGGVFPAIFGTVLMVFMMTIIVTPLGIIAAIYLREYASQ
                     GVITQTVRIAVNNLAGVPSIVYGVFGLGFFVYFLGGNIDKLFYPESAPAPVFGTPGLM
                     WSAITLALMTLPVMIVSTEEGLSRIPRSLKEGSLALGATKAETLWRVILPMTMPSIMT
                     GMILAIARAAGEVAPLMLVGVVKLAPTLPVDLNAPFIHLERKFMHLGFHIYDVGFQSP
                     NVEAARPLVYATAFLLVLIVAALNLTAVALRNRLREKYKALES"
     gene            2965..>3650
                     /gene="pstB"
                     /locus_tag="PLY05_07950"
     CDS             2965..>3650
                     /gene="pstB"
                     /locus_tag="PLY05_07950"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017394957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNC03274.1"
                     /translation="MADEDICLQTQNLNLFYGQKQALFDINMTIPRKRVTAFIGPSGC
                     GKSTLLRTFNRMNDLVDGCRIDGKILLDGNDIFDRGMDITSLRRRVGMVFQKPNPFPK
                     SIYENVAYGLRLQGVNKKSVLDDVVEHSLKGAALWDEVKDRLHESALGLSGGQQQRLV
                     IARAIAIEPEVLLLDEPASALDPISTLKIEELIHELRDRFTILIVTHNMQQAARVSDY
                     TAFMYLGKLI"
BASE COUNT          966 a          663 c          846 g         1175 t
ORIGIN      
        1 aatgcaccga ttaatgcctt gtcggtgtct gctcgttccg aaggaatctt tgttcaagcc
       61 gcagatagtg ccagtttttg gtctttacat accgaacatc ctgatatttc tatgaagtca
      121 gcatggggta aagtttggta tgaatcttac ccagaaccaa gttatacatg gcaatctacc
      181 tctggtaatg ccgactttga aggcaaaatg tcattaatgc ctttaacctt tggtacactc
      241 aaagctgctt tttatgccat gttattggct gcaccgttag cgattttagg ggctatgtac
      301 acggctgtgt ttatgtctcc agccctacgc actaaagtca aaccagcaat tgagttaatg
      361 caagctttac caacggttat tttaggtttc ttagcaggtt tatggttagc accaacctta
      421 gaagccaatt tgccgggtat tgtcagttta ttaattatta cacccatcat cattttagtt
      481 tgtggtttta tttgggtaca aattccctta gaaaagcgca accgtattcc tgatggttgg
      541 gcacctgtga ttttaattgc acccattttg ttaggtggtt ggatttcttt tgcctcctca
      601 caaaccattg aattgcagtt ttttggtggt gatattcgtc actggttaac ctatgaagca
      661 ggtatcacct ttgaccaacg taactctcta attgttggct tggcgatggg ttttgcagtg
      721 attgccccta ttttctccat tgctgaagat gccttatttg ccgtacctcg tcatttaacg
      781 aatggttctt tagctttggg tgcaacccaa tggcaaacct taaccagagt tattttacca
      841 actgcctcac ctggtatttt ctcggcggtg atgattggtt ttggtcgtgc tgtgggcgaa
      901 acgatgattg tgttaatggc cacaggtaac acagcggtga tggaatggaa catttttgaa
      961 gggatgcgta ccttatcagc caatgtcgcg gtagaaatgg gtgaggccga agtcggttca
     1021 acacacttta gaattttatt cttatcagcc ttagtgttgt ttgttttgac ctttatttta
     1081 aatactgttg cagaagtggt aagaagccgt ctgcgtaaaa agtatggttc tttatgaggc
     1141 ttaatccatg aagttacgtg aatattttag aagcggtgaa gcgtgggttt ggctaaatgc
     1201 aggtgcagta gccatttctc tgattatggt cattgggatt ttgggtttaa tcgctgtgcg
     1261 tggtttgggg catttttggc cttcagatgt tatgcaagcc caatatactt atcagggaca
     1321 aactgaaacc attattggtg agctttacga cgaaaaagtg gatactgcta agcgcgtgcg
     1381 tgaggcaggt attgagattc ctgaaaatgt tagcaccgtc actcgttact ccattaaaca
     1441 aggtaacaaa gatgtgactg gccaagattt tcgttgggta ttagccagta atttgcaaaa
     1501 gcaacaatat cctgccgata ttgttgcttt agaacgttat gaatggggcg cgttttatgg
     1561 ccgtttaaaa gcggttaaag aaaatggtgt ggtgattgcg gatggcgata cagcatggca
     1621 acaattacaa acacgtttac aacgtaccaa tgattttcat gaccaaattg cggatattga
     1681 aaaaggtgaa attggtcgta ttaattttca actcgaaaaa ctacgtttac aagagcgtaa
     1741 gttggaatta gacaagcaat taactgatgc agctcgtgct gatttacaag cgaaacgcga
     1801 tgagcaaaac aaaaagtacg aattgttaaa ggcaaaatta gagcaattaa atcgtgatat
     1861 ggagcgtgac acggcgattt ttgtggtggc aaatggtacc gagaaagaaa ttgccttaag
     1921 caaaattgtt gctgctaata agccaaatga tatgagcgtc tttgccaaat tagctgcgta
     1981 tggccataac gcatgggcgt tcttgagtga cgatccacgc gaagccaata ctgaaggtgg
     2041 tgtattccct gcaatttttg gtacggtgtt aatggtattt atgatgacca ttattgtcac
     2101 gcctttagga attattgcag caatctattt gcgtgaatat gctagccaag gagtaattac
     2161 tcagacagtt cgtattgccg taaataactt agcaggcgta ccatctattg tgtatggtgt
     2221 gtttggttta ggtttttttg tttacttttt aggtggcaat attgataaat tgttttatcc
     2281 tgagtcagca ccagcgcctg tttttggtac accagggcta atgtggtcag ctattacctt
     2341 agcactaatg actttgcccg tgatgattgt ctcaaccgaa gaaggtttgt cacgtattcc
     2401 tcgtagctta aaagaaggtt cgttagcttt aggtgcaacc aaagccgaaa ccttatggcg
     2461 tgtgatttta cccatgacca tgccgagtat tatgacaggt atgattttgg cgattgctcg
     2521 tgctgcgggt gaagttgcgc cattaatgtt ggtgggcgtg gttaaattag ctcccacctt
     2581 acctgttgac ttaaatgcgc catttattca cttagagcgc aaatttatgc acttaggctt
     2641 tcatatttat gacgtaggtt ttcaaagtcc aaacgtcgaa gccgcgcgac ctttagtgta
     2701 tgccactgcc tttttattgg tattgattgt ggctgcttta aatttaacgg ctgttgcttt
     2761 acgcaaccgc ttgcgtgaaa aatacaaagc acttgagtct taatatcaag atttagcttt
     2821 aacaaagcta aatgtgttta gagtgaatac agtatgactt tagaattgga aaagccaacc
     2881 atgaatgctc caaaatctgc cactccaacg cctgcctatt cgattgatat ggaggcttta
     2941 ggtcgccaaa atcgtaattt gaacatggca gacgaagata tttgtttgca aacgcaaaat
     3001 ctcaatttgt tttatgggca aaagcaagct ttgtttgata ttaatatgac cattcctcgt
     3061 aagcgtgtga cagcgtttat tggaccttcg ggttgtggta aatcaacctt attgcgtacc
     3121 tttaatcgca tgaacgattt ggttgatggt tgtcgtattg acggtaaaat tttattagat
     3181 ggcaatgata tctttgaccg tggtatggat attactagct tacgtcgtcg cgtaggtatg
     3241 gtgtttcaaa agcccaatcc ctttccaaaa tccatttatg aaaatgtcgc ttatggtttg
     3301 cgtttgcaag gcgtgaataa aaagagtgtg ttggatgatg tggtcgaaca ctcattaaaa
     3361 ggcgcggctt tatgggatga ggtaaaagac cgtttgcatg aatcagcttt aggtttgtca
     3421 ggtggtcaac aacaacgttt ggtgattgcg cgtgccattg ctattgagcc agaagtgtta
     3481 ttgcttgatg agccagcttc ggcacttgac ccgatttcga ccttaaaaat cgaagagttg
     3541 attcatgaat tacgtgatcg cttcactatt ttgattgtga ctcataatat gcaacaggca
     3601 gcgcgtgtgt ctgattacac agcctttatg tacttgggta aattgattga
//
LOCUS       DAOQMB010000231         3636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13266_length_3636_cov_7.712929, whole genome shotgun sequence.
ACCESSION   DAOQMB010000231 DAOQMB010000000
VERSION     DAOQMB010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13266_length_3636_cov_7.712929"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1057)
                     /locus_tag="PLY05_07955"
     CDS             complement(155..1057)
                     /locus_tag="PLY05_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005292459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03275.1"
                     /translation="MSLQQQITTHIVKQSLRASFKFTSTLPLPVSALRTGMELGASLF
                     PVNQQVGVNRTVIGGVAAELVSSQPQAKQVILHCHGGAFFTGSAKTHRALASDLSVLS
                     GADVYMLDYRLAPEHPYPAALDDAYSAYMALLDKGYQAQQIILAGDSGGCAHILNLAL
                     RLREQGQALPAGLAMISPFVDLTLSMPSVVRHKRLDPMVTAHALKRGADAYRGQIAAN
                     DRRVSPLFADLTGLPPLLIQVGEQEILLDDALALERIARAAGVEVTCEVYDSMWHNFQ
                     MFSQFVHKADFALQQIGRFVKRCA"
     assembly_gap    1058..1157
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1159..2670)
                     /locus_tag="PLY05_07960"
     CDS             complement(1159..2670)
                     /locus_tag="PLY05_07960"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008941185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC03276.1"
                     /translation="MTANAQNLGQSVAKGQKTKLWQVIVIGSGFAGLGAAIKLREAGI
                     NDFIVLEKANELGGVWRENTYPGCACDVPSLLYSYSFAPNPHWSRVFAEQAEIKAYLQ
                     KVAQDYGVIPHIYMQQEMLDAAWDNQENLWRVKTQDQEYQARFVIMACGPMHEPVYPK
                     IKGLADFKGTIFHSARWRHDYDLTGKRVAVIGTGASAIQFVPKIQPKVAELVLFQRTP
                     QWILPKLDQALPPIAQKLFKNLPLTQLAMRGAIYSVFETLNGGMHHPNVMKQFQRLAL
                     FNLHKTVKDPVLRKKLTPNFIIGCKRVLQSNEWYPAMIQPNVTHVFEGVKEIKAHSIV
                     GDDGVQRPVDAIILGTGFEISAPPIAQRVRGVTGKSMAEVWQGSPEGYMGTMVAGCPN
                     GFLMFGPNLAVSSSAFLIIEAQLTYIVDALKQAEQYGLVKIEVDPVRQQQFNQTVQAA
                     LQNTVWNKGGCQSYFIDVNGRNSTLWPWSTIEMRKQLSRFNLDEYLCKTEVTA"
     gene            2913..3542
                     /locus_tag="PLY05_07965"
     CDS             2913..3542
                     /locus_tag="PLY05_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005190658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC03277.1"
                     /translation="MNDDSSLSRAERNRQQLRSDILQAAFIEFAERGYHQTAISDIAK
                     RLGIGHGTFYRHFTNKRDILEHVIDDIASKIIATLEAENAPDAVTSLEAYKEQCARIA
                     NRLSEVLTANPAIARLILLEATSIDAEMTKRLMNLIDWGGQLVAAYMINGVKLGYFRK
                     DLDCQSTGHAVVGMILVCALRFLNNPNHPDEQQRLAIAVQRLLVDGIKS"
BASE COUNT         1048 a          805 c          679 g         1004 t
ORIGIN      
        1 cttaaaaatg caaagcaagc gtatgcagtt attgatgcaa caaatggtac aacccatacc
       61 tgcctaaaca taaacaggac ttacgcattg tgctgcaatg cgtaagtcct aataaagtgt
      121 ttcacttata aaaaagagcc gttaggctct ttttttatgc acatcttttc acaaaccgcc
      181 ctatttgctg caaggcaaaa tctgctttat gcacaaactg gctaaacatt tgaaagttat
      241 gccacatact gtcatagact tcacaagtca cctctacccc agctgctctc gctatacgct
      301 ctaaagctaa ggcatcatcc aataaaatct cttgctcacc cacttgaatc aataacggtg
      361 gtaaacccgt taaatcggca aacaaaggcg acacacgtcg atcattagcc gcaatttgac
      421 cacgataagc atcagcacca cgctttaagg catgtgcggt gaccatcgga tctaaacgtt
      481 tatgacgtac aacagacggc atacttaagg ttaaatccac aaatggcgaa atcatggcta
      541 aacctgctgg caaggcttgg ccttgctcgc gcaagcgtaa agcaagattg agaatatgcg
      601 cacagccacc tgaatcacca gccaagataa tttgctgtgc ttgatagcct ttgtctaata
      661 aagccatata ggcactatag gcatcatcaa gcgcagcagg atatggatgc tcaggggcta
      721 aacgataatc aagcatataa acatctgcac cactcaacac actcagatca ctggctaaag
      781 cacgatgagt tttggctgag cctgtaaaaa atgcaccacc atgacaatgc aaaatgactt
      841 gtttggcttg aggctggcta ctgactaatt ctgccgctac cccaccaatc accgtacgat
      901 tcacacccac ttgttgattg acaggaaata aactagcccc caactccata cctgttctta
      961 aagcactgac aggtaaaggt aaggtgctgg taaacttaaa actggctctt aaactttgtt
     1021 taacgatgtg ggtggtaatt tgctgttgta aactcatnnn nnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnctc atgctgtcac ctcggttttg caaagatatt catctaaatt
     1201 aaaacgactc agttgtttac gcatttcgat ggttgaccac ggccacaagg tactatttcg
     1261 accattaaca tcaataaaat agctctgaca accgcctttg ttccacactg tattttgcaa
     1321 agctgcttgt accgtttggt taaattgttg ttggcgaaca ggatcaactt ctattttcac
     1381 taagccatat tgttccgctt gtttgagtgc atcgacaata taagtcagtt gcgcttcaat
     1441 gattaaaaat gccgaagagc tgactgctaa gttagggcca aacattaaaa agccattcgg
     1501 acaacctgcc accatcgtcc ccatatagcc ttcaggactg ccttgccata cctcagccat
     1561 acttttacct gtgacaccac gcacgcgttg cgcaatcggt ggagcactaa tttcaaaacc
     1621 tgtccccaaa ataatcgcat ctacaggtct ttgcacgccg tcatcaccaa caatactatg
     1681 agctttgatt tctttaacac cctcaaatac atgagtcaca ttgggctgaa tcatggcggg
     1741 ataccattca ttcgattgca acacacgttt acagccaata ataaaattgg gggttaactt
     1801 tttgcgtaaa acagggtctt taacggtttt atgcaggttg aataaggcta aacgctgaaa
     1861 ttgtttcatc acgtttggat gatgcatgcc accatttaag gtttcaaaaa cgctatagat
     1921 tgcgccacgc atcgctaatt gggttaacgg caaattctta aacagttttt gggcaatcgg
     1981 cggtaaggct tggtctaatt taggcaaaat ccattgtgga gtgcgttgaa acaacaccaa
     2041 ctctgctact ttgggttgaa tttttggcac aaactgaatt gctgatgcgc ctgtaccaat
     2101 cactgcaaca cgtttgcctg ttaaatcata atcatgtcgc caacgtgctg aatgaaaaat
     2161 cgtaccctta aaatcagcta aacctttaat ttttggatag acaggctcgt gcatcggccc
     2221 acaggccata atcacaaaac gtgcttgata ttcttggtct tgagttttta cacgccaaag
     2281 attttcttga ttatcccatg cggcatccaa catttcttgt tgcatataaa tatgcggtat
     2341 taccccataa tcttgcgcta ctttttgcaa ataggcttta atttcggctt gttcagcaaa
     2401 gacgcgtgac caatgcggat tgggggcaaa agaatacgaa tacaacaatg atggcacatc
     2461 acacgcacaa cctggatagg tattctcgcg ccatacccca cccaactcat tggctttttc
     2521 taagacaata aaatcgttaa tgcctgcctc acgcaattta atggctgctc ccaaacctgc
     2581 aaaaccgcta ccaatgacaa tgacttgcca taattttgtt ttttgaccct tagcgactga
     2641 ttggccaaga ttttgtgcat ttgctgtcat ctttcacccc aaacttacaa tacggacata
     2701 caattccaat acggaacaag atgtcacatt tagaatttat tgtcaatttt tatttattag
     2761 tatgaatctt gtgttattct ttagtaataa attcaaacct ctacgccctc accccaatcc
     2821 tctccaacaa tgagaggaag ttctctctca ctgagaaaga gaactagaat gaagggggaa
     2881 acgttaatac gctaaaaatt aggttatatg ccatgaatga cgatagctct ttgagccgtg
     2941 ctgaacgcaa tcgccaacaa ttacgcagtg atattttgca ggccgctttt atcgagtttg
     3001 ctgaacgtgg ttatcatcaa acagctattt ctgatattgc taaacggttg ggaattggtc
     3061 atggcacgtt ttatcgtcac ttcaccaata aacgcgatat tttggaacat gtcattgatg
     3121 acattgccag caaaattatt gcgacgctag aggccgaaaa tgcgcctgat gcggtgactt
     3181 ctttagaagc ctacaaagaa caatgtgcgc ggattgccaa ccgtttatct gaagtgttga
     3241 cggctaaccc tgcgattgct cgactgattt tgctagaagc cacctcgatt gatgccgaaa
     3301 tgaccaaacg cttaatgaac ttaattgatt ggggtggaca gttagtggcg gcctatatga
     3361 ttaatggcgt caaacttggt tactttagaa aagatttaga ttgtcaatcc acaggtcatg
     3421 cagtagttgg catgattttg gtttgtgcct tacgcttttt gaataatcct aatcatcctg
     3481 atgaacaaca acgtttagcg attgcggtac aacgcttatt agtcgatggg attaaaagtt
     3541 aaatccttaa tatttatcag gtgtttattc agcaaaatcc ctttatgatg caagcaagat
     3601 tttcgtgagc atcatttatg gcgactttac aaaccc
//
LOCUS       DAOQMB010000232         3614 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13398_length_3614_cov_7.454060, whole genome shotgun sequence.
ACCESSION   DAOQMB010000232 DAOQMB010000000
VERSION     DAOQMB010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13398_length_3614_cov_7.454060"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1024
                     /locus_tag="PLY05_07970"
     CDS             479..1024
                     /locus_tag="PLY05_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03278.1"
                     /translation="MPRRTWTNEQRAAQAEKMRKRKIWELSTGPKTTKGKQKVAQNGR
                     KNATGKNLTKDEYIALIKQMNPKLVEDSELLEEMLSGITFKDDKATKKPNKDIEPMIR
                     TITVVNGVFREVKRDMWTIKDVETARKKVDVIIKRLSQVVNMTMDTCQQLHNLHKKYE
                     YEEEAAKIKKRASFNNPFPHG"
     gene            1046..1939
                     /locus_tag="PLY05_07975"
     CDS             1046..1939
                     /locus_tag="PLY05_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03279.1"
                     /translation="MKAYPYPIQQPVNAPQPKLPPPKYDQWGKINPDLLAQIYVVDKD
                     GNSEKGGYRVVCAITEGTFEVGSEYSSPFDANPDQRLPTLMGMIQSGEGVTALGNIMA
                     NIGATETGTQIGGVAAGVGAALNVATGGLSGSFFGGVVSKAVNLKNEIQSLEGKTNLT
                     KVNSTQIFVNTKPIEISVTLYFRAWRDAKKEVEEPVSYLERWALPRSLWDRSIVESVT
                     GRIAKGESGLSGLFPSDAPPFVALQYAKKTFLPFTLRNVSKPLVVERDSQMNALAYVV
                     NAQLSSRTAWDASDIRKLYGG"
     gene            2034..2519
                     /locus_tag="PLY05_07980"
     CDS             2034..2519
                     /locus_tag="PLY05_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03280.1"
                     /translation="MSAYMSTYQSNHQAGEAYAFAAMLLEQQANITRLGGYHPLVDTL
                     LAIVKEQNIATVEAFYIVQNQQKQLFAKTEQDAHKAMAAYDEHIAKHTEPLKFLRAKK
                     NDLEAKLGAVTGKINSYPNVKKNKEAILTQQGFSKEQIIQIISLDDTLPQLQQEQQAI
                     S"
     gene            2687..2914
                     /locus_tag="PLY05_07985"
     CDS             2687..2914
                     /locus_tag="PLY05_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03281.1"
                     /translation="MFGQGAFSAPKKPTVDPVSSRVVWRISYVVVLNGRPISRYKDVG
                     NHSAIGAMIGSILSGGDKVSVFTVQKIKVAV"
     gene            2911..3414
                     /locus_tag="PLY05_07990"
     CDS             2911..3414
                     /locus_tag="PLY05_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03282.1"
                     /translation="MNTKEKAKELAKLAQSLKNTCYHEVGHCVVARELGIKSEWEIIF
                     EYEKDKDYIYTQLLGVTSFKRDNSLLTDIRGRIIGLAGVVAEAYLLDSNVEPLQIYRD
                     IKVGLIELSDTDRVFVSDYFSRQDVGLCLDIVKQRFDLITQDAESGFSYYRYLLDECS
                     SLLDEEN"
BASE COUNT         1178 a          653 c          763 g         1020 t
ORIGIN      
        1 tgatgttatc cgcgatacca tacgccaaga actacaccac taatcaacac aggggcaaat
       61 aagccccttt ttattggcct catgtttatt tactggctca aaatcgacac gttttaggct
      121 tatctttaag gcttaaacat agccttatca ctggaaaaaa acaggggtca aactaatagt
      181 gagccttttt ccaatttttc ggtatttttc aagtgctttt ttcggtatca aaaccttagc
      241 tatcaatgct tagaccacta ttgaaatact ggaaaaatca actatcagtt aaaaactatc
      301 agtttgcttg ttatttttgt cctatctgta aaaaagcctt atttttcaat acttaaaagc
      361 ctacattatg ttttagtggc ggtgtactgg aacacctatg agggcggtgt ccttgtccta
      421 tgcgaaaata tccttagacc caaactaata gttatctttt cggtaaaaag gtgatgtcat
      481 gcctagacgt acttggacaa acgaacaaag agccgcgcaa gctgaaaaaa tgcgtaaacg
      541 taaaatatgg gagttatcga cagggccgaa aaccaccaaa ggcaaacaaa aggttgccca
      601 gaatggccgt aaaaatgcca caggtaagaa tttaaccaaa gatgaataca ttgccctcat
      661 taagcaaatg aatcctaaac tggttgaaga ttcagaacta ctggaggaga tgcttagcgg
      721 tattaccttt aaagacgaca aggccactaa aaagcctaat aaagacattg aacccatgat
      781 tagaactata actgtggtta atggcgtatt cagagaggtt aagcgcgata tgtggacaat
      841 taaggacgta gagacagcgc gtaaaaaagt tgatgtgatt attaaaaggc tatctcaagt
      901 cgtgaatatg acaatggata cttgccaaca actgcataac ctacacaaaa aatatgaata
      961 tgaggaagaa gcggcaaaga ttaaaaaaag agcctctttt aacaatccat tccctcatgg
     1021 ttaatccata aaaaaggcat aaattatgaa agcataccct tatccaatcc aacagccagt
     1081 caatgcacct caacccaaac taccacctcc aaaatacgac caatggggaa aaataaaccc
     1141 tgatttattg gcacaaattt atgttgttga taaagatggc aacagtgaaa aaggggggta
     1201 tcgtgtggtt tgtgccatca ccgaaggcac atttgaagtc ggtagtgaat atagtagccc
     1261 ctttgatgct aatcccgatc aacgcttacc tactttaatg ggcatgattc aaagcggtga
     1321 aggcgttacg gctcttggta atattatggc caatattgga gcaacagaga caggtactca
     1381 aattggagga gtggcagcag gtgtaggtgc agcattgaat gtggccactg gtggtctgtc
     1441 aggttctttt tttggtggag ttgttagtaa agcagtaaat ttaaaaaatg aaattcaatc
     1501 gcttgaaggc aaaactaact taaccaaagt caacagtaca caaatttttg tgaatactaa
     1561 gcctatcgaa ataagcgtca ctttatattt tcgtgcgtgg cgagatgcta aaaaagaagt
     1621 tgaagaacca gtcagctacc tagaacgatg ggcattacct cgctctttat gggatagaag
     1681 tattgtagaa agcgtgactg gacgtattgc aaaaggtgaa agtggattaa gcggtttatt
     1741 tccctccgat gcaccgccat ttgtcgcttt gcaatacgct aaaaagacct ttttaccctt
     1801 taccttgcgt aatgtctcta agcctttagt cgttgaacgc gatagccaaa tgaatgcttt
     1861 ggcttatgtg gttaatgctc aattatctag tcgtaccgca tgggatgcgt ccgatattag
     1921 aaaattatac ggaggctaaa gcatatagat tatgtactgg aactatcagc aaactaatag
     1981 atacaaactg agtgacaata ctcgcaccta aacaccttta gagagttatt tttatgtccg
     2041 cttatatgtc cacttaccaa tcaaatcatc aagcaggtga agcctacgcc tttgcagcca
     2101 tgttattaga acagcaagct aacattactc gtcttggggg ttatcatccg ttggttgata
     2161 ccttactggc aatcgttaaa gaacagaaca ttgccaccgt tgaagccttt tatattgtgc
     2221 aaaaccaaca aaagcagcta tttgccaaaa cagaacagga cgcacacaag gcaatggcgg
     2281 cttatgatga acatattgcc aagcacaccg agcctctcaa gtttttacgc gctaagaaaa
     2341 acgacctaga ggcaaaacta ggcgcggtga ctggcaaaat aaatagctat ccaaacgtca
     2401 aaaagaacaa agaggcaata ctcactcaac aaggctttag caaagagcag attattcaga
     2461 ttattagcct agatgatacc ttgccccagt tacagcaaga acaacaggcc attagttaag
     2521 aactggcaga agtgtcggca gactttcaaa gcatttatga cacggctagt aaaattaaga
     2581 gcgacttgtt ttacaggcta tcgagagaag ccgcttaatg acaaagggtt gcgtttaaaa
     2641 cgtgaccctt tggttaattg tttgaatttg agcaagtgag gtttttatgt ttggacaagg
     2701 cgcattttca gcaccgaaaa agccaacggt tgacccagtg agcagtcggg tggtatggcg
     2761 tatcagttat gttgttgtgc ttaatgggcg accaattagt cgttataaag acgtaggcaa
     2821 ccattcagcc attggtgcaa tgattggtag tattttgtca ggtggtgaca aggtatctgt
     2881 ttttactgtg caaaaaatta aagttgcggt atgaatacca aagaaaaagc taaagaatta
     2941 gcaaaactag cacaatcatt aaaaaatacc tgttatcacg aggtggggca ttgtgtcgtt
     3001 gcaagggaac taggtatcaa atcggaatgg gaaattattt ttgaatatga gaaagataaa
     3061 gattatatct atacccaact tttaggggta acgtctttta agcgcgataa tagtttacta
     3121 acggatatta gaggccgcat tattggcttg gcaggggtgg tggcagaggc ttatttgttg
     3181 gatagcaatg tcgaaccttt acaaatttat cgtgatatta aagtcggcct aattgagcta
     3241 tcagacacag acagagtttt tgtatctgat tatttttcta ggcaagatgt tggcctttgc
     3301 ttagatattg ttaaacagcg ttttgattta attactcaag atgctgaaag cggattttct
     3361 tattatcgct atttgcttga tgagtgtagc agccttttag atgaagaaaa ttaaacacac
     3421 cgccaccatg taggattttg ctatttttag ggggtcactt ttcttttttg gggcattata
     3481 gggggcattt ttgatatgca ttaaaaataa atattatata aaacaattat ttaagttaat
     3541 tttgtgattc ctgtcgagta aaaactaata aataatcggt gttattgttg attgcacagg
     3601 ctatttgtgt agcg
//
LOCUS       DAOQMB010000233         3587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13565_length_3587_cov_16.569083, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000233 DAOQMB010000000
VERSION     DAOQMB010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13565_length_3587_cov_16.569083"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..79)
                     /locus_tag="PLY05_07995"
     tRNA            complement(3..79)
                     /locus_tag="PLY05_07995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43..45),aa:Val,seq:gac)
     gene            complement(127..2142)
                     /locus_tag="PLY05_08000"
     CDS             complement(127..2142)
                     /locus_tag="PLY05_08000"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HNC03283.1"
                     /translation="MNRLASRYALLSALFIVILPHWQRLDLWLDIAILLCFLWRIPAI
                     EQRLPLPNTLIKALLVIASIAAIKYAYNTWFGPEAGTAFLILCAALKLLETRDERDCY
                     VVLTLSYFILAAQFLFEKSLWTTLYTALGIIWVTMAYVAQNQSISIKIALKKSLMLLG
                     QALPLMIVLYLFFPRLPPLWSLKLTEGTGKTGMSDNMSPGDLAKLSQSTELAFRVEFE
                     DKKNIPPKSELYWRGLTLSNFDGTTWRTSQLSVFADENAAWSEFPLPQWAETQIQIVQ
                     QKPTHYKVILEPTDQVWLYSLMVPYSRDKGIGLTRDFRLVSRVPVFERFSYEVMQFKK
                     ISLDANLPFWLRQEMLKLPEKGNATAHQMAQQWRKFYASDEAYIQAVLAWFRKSNFYY
                     TLEPPLLGENRIDDFLFKTKRGFCEHYSSAFTFLLRAAGIPARVVVGYQGGEWSPTGD
                     SWQVRQMDAHAWVEAWLPNKGWTLLDPTGAIAPERIEKGMTDLADNQQVWGSSTLSTL
                     KYSNYKLFSQLRNMADYLNYRWQKDIVGYDSAQQDQLLLKLLGDSSIWKRIAIMFVAL
                     FVIAGLLAVWTILKGQKRHHPADKVILNLSYRLTKRGLSRQQGEGVVAYLQRLQIAQP
                     HWQEVLIAIEQEYCLLRYQQNASSSTQKLKQLLHKLPSYQPQKTKQV"
     gene            complement(2139..3110)
                     /locus_tag="PLY05_08005"
     CDS             complement(2139..3110)
                     /locus_tag="PLY05_08005"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNC03284.1"
                     /translation="MAIIDRLKQAYKTRWQQWLSHRIPRAKEVILNQRRVFIFLSSYG
                     GMMSLLLLALFIAGINYANNLILGFCFFLGSLLVIGIHHTFANLSGLKINAIVAGEAF
                     AGDVVAFTIRLTPTNKRLYYSLLLDWGGVKEQVEIVDGQTEVTLFLRTEQRGRFQPPR
                     LKMTTFYPLGLLRAWTWLDLDLTAIVYPQPIASDELPSGSGDKEQAEHSQKRKVGIED
                     FEGLKKFNEGDPLAHVSWKHLARGQGMLVKTYSEPVTGSNTLDYLAFQGLDKENRLSR
                     LAWWVLRLTEQQQPFALKLPHKKIEVGTGHQHQQQCLRALALFEEPL"
     gene            complement(3114..>3587)
                     /locus_tag="PLY05_08010"
     CDS             complement(3114..>3587)
                     /locus_tag="PLY05_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNC03285.1"
                     /translation="QMGTFPLPESQLDRFLMRIQLGYPDPRAERELLTGKDRRQMLTL
                     LPALADAERLQAWQEQILTIHLSDAVIDYLQRLVIHTRQSRDFPHGLSPRGLLALKRA
                     TQAWAFIEGRNYAIPEDVQAVLPAVAEHRLRGSMEDQQKMHSLTSQLLNAVDVIE"
BASE COUNT         1121 a          769 c          636 g         1061 t
ORIGIN      
        1 tttggtagac gtaactggac tcgaaccagc gacccccacc atgtcaaggt ggtgctctaa
       61 ccaactgagc tatacgtcta aagagggcat aattctatat aagcccttac aaataagcaa
      121 gtctttttat acttgcttcg ttttttgtgg ttgataactg ggcaatttat gaagtaattg
      181 cttgagtttt tgggtacttg atgatgcatt ttgctgataa cgcaacagac aatattcttg
      241 ttcaatagct ataagaactt cttgccaatg tggctgtgca atttgtaaac gctgcaaata
      301 tgccacgaca ccttctcctt gctgcctaga taaacctcgc ttggttaaac gatatgacaa
      361 attaagaatc actttatctg ctggatgatg tcttttttgg ccttttaaaa ttgtccacac
      421 tgccaacaac cctgctatca caaataaagc aacgaacata atggcaatac gtttccaaat
      481 tgaggagtca cctaagagct ttaacaacag ttgatcttgt tgagccgaat cataacccac
      541 aatatctttt tgccagcgat aatttaaata atctgccata tttctcaatt ggctaaacaa
      601 tttataatta ctatatttta aagtgcttag agttgaactt ccccaaactt gctgattatc
      661 tgctaaatct gtcatgcctt tttcaatacg ttcaggtgca atagctcctg tagggtctaa
      721 tagtgtccaa cctttattag gcaaccatgc ttctacccat gcatgggcat ccatttgtct
      781 tacttgccac gagtctcctg ttggactcca ctctccacct tgatacccaa cgacaacacg
      841 agcaggaata cccgctgccc gtaataaaaa agtaaatgcc gatgaataat gctcacaaaa
      901 tccgcgctta gttttaaata aaaaatcgtc aatccgattt tctcctaaca aaggcggctc
      961 taaggtgtaa taaaaattac tttttctaaa ccatgctaat acagcctgaa tatatgcttc
     1021 atccgaagca taaaactttc gccattgttg agccatttga tgcgcagttg cattaccttt
     1081 ttcgggtaac tttaacattt cttgtcttaa ccaaaatggc aaattggcat ccaaacttat
     1141 ttttttaaat tgcatgactt catagctaaa gcgttcaaac acaggaacac gactaaccaa
     1201 tctaaaatca cgcgtaagac ctattccttt atctcgagaa tatggcacca tcaaactata
     1261 caaccacact tggtcagttg gctccaaaat aactttataa tgtgtgggtt tttgctgaac
     1321 aatctgtatt tgagtttcag cccattgagg taaaggaaac tctgaccatg ctgcattttc
     1381 atcggcaaaa acagagagtt gacttgtgcg ccatgtcgtt ccgtcaaaat tacttaaggt
     1441 taaacctcgc caatataatt ctgatttagg tggaatattt tttttgtcct cgaactcaac
     1501 tctaaaagct aattcagtag attgacttaa tttagccaaa tctcctggag acatattatc
     1561 ggacattccc gtttttcctg ttccctccgt tagcttaaga ctccataaag ggggtaaacg
     1621 cggaaaaaat aaatataaaa cgatcatcaa tggcaaagct tgccccaata acatcaagga
     1681 ttttttaagg gcaattttga tagatatact ttgattttga gccacataag ccattgtcac
     1741 ccaaataata cccaaagcag tatataacgt tgtccataaa cttttttcaa agagaaattg
     1801 tgctgccaaa ataaaataag acaaggttaa aaccacataa cagtcacgct catcacgggt
     1861 ttctaataat tttaatgccg cacacaaaat taaaaatgcc gtgcctgctt cggggccaaa
     1921 ccatgtgttg taagcgtatt taattgccgc aatactggct ataactaaca aagctttaat
     1981 aagagtattg ggtaacggta aacgttgttc aatggcagga atgcgccata aaaaacacag
     2041 caaaattgca atatcaagcc acaaatctaa acgttgccaa tgtggcaaaa tcactataaa
     2101 taatgctgat aataaagcat aacgcgaagc caaacgattc ataatggctc ctcaaataag
     2161 gccaacgctc gcaaacattg ttgttgatgt tgatggcctg tacctacttc tattttttta
     2221 tgcggcaatt ttaaggcaaa tggctgttgt tgctcggtta atctaagcac ccaccatgcc
     2281 aaacgcgata atctgttttc tttatctaag ccctgaaagg ctaaataatc taaagtattg
     2341 ctacctgtga caggttcact ataagttttg actaacattc cttgtccacg tgctaaatgt
     2401 ttccatgata catgggctaa tggatcaccc tcattaaatt tttttaagcc ttcaaaatct
     2461 tcaatgccta ctttgcgttt ttggctatgt tctgcttgtt ctttatcacc agagccagaa
     2521 ggcaattcat ctgaagcgat aggttgagga tagacaattg cggttaaatc taagtctagc
     2581 catgtccatg cgcgtaataa acccaaggga taaaatgtgg tcatttttaa acgcggcggc
     2641 tgaaaacgtc ctcgttgctc agttcgtaaa aataaagtca cctctgtttg accatctaca
     2701 atttcaactt gctctttaac tcccccccaa tccaataaca aagaataata taagcgttta
     2761 ttggtgggtg ttaatcgaat cgtaaaggcg acaacgtccc ctgcaaaagc ctcacctgcg
     2821 acaatggcgt taatttttaa ccccgataaa ttggcaaaag tatgatgaat ccctatcacc
     2881 aataaactac ccaagaaaaa acaaaaaccg agtattaaat tgttagcgta gtttatgcct
     2941 gcaataaata atgccaataa caataacgac atcataccgc cataagacga taaaaaaata
     3001 aatacacgtc gttgattaag aatgacttct ttggctcgtg gaatgcggtg gcttaaccat
     3061 tgctgccaac gtgttttgta tgcttgtttt aggcggtcta taattgccat gacttactca
     3121 atgacatcaa cagcgtttaa taattgactg gttaatgagt gcatcttttg ttgatcttcc
     3181 atactgcctc ttaaacgatg ctcagcaacc gcaggcaaaa cggcttggac gtcttcggga
     3241 atagcataat tacgtccttc aataaacgcc catgcttgtg tggcacgttt aagagctagc
     3301 aaacctcgcg gagataaacc atgtgggaaa tcgcgacttt gacgggtgtg aatgactaaa
     3361 cgttgtaaat aatcaatcac tgcatcactc aaatgaatag tgagaatttg ctcttgccat
     3421 gcttgcaaac gttcggcatc agccaacgca ggtagcaaag tcaacatttg gcgacggtct
     3481 ttaccagtta ataattctcg ctctgcacgt gggtctggat aacctagctg aatacgcatt
     3541 aaaaaacgat ctaattgcga ttcaggcaat gggaaagtgc ccatttg
//
LOCUS       DAOQMB010000234         3583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13599_length_3583_cov_4.145692, whole genome shotgun sequence.
ACCESSION   DAOQMB010000234 DAOQMB010000000
VERSION     DAOQMB010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13599_length_3583_cov_4.145692"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..767)
                     /gene="cas1"
                     /locus_tag="PLY05_08015"
     CDS             complement(207..767)
                     /gene="cas1"
                     /locus_tag="PLY05_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNC03286.1"
                     /translation="MNTLFVDKRGIELKVDNNALLVYQDKTRINTVPLSPLKRIFIQG
                     DVQLSASVLAKLGEYKIGVVVLYGLKAEPTLLMPCYGLDAQRRQAQYALSQQDLLCVA
                     FAKQLVAQKIQGHRQLLQEMERCHPQHKSQFYNAIKRLDGIVIKPLKQASNIDTVRGL
                     EGAAAACYFETLAYGLPASLNFDGRN"
     gene            complement(803..1087)
                     /gene="cas2"
                     /locus_tag="PLY05_08020"
     CDS             complement(803..1087)
                     /gene="cas2"
                     /locus_tag="PLY05_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNC03287.1"
                     /translation="MSRDLYLVCYDISDEDTLQQVRQYLQGWRVQGQKSAMECWFTAN
                     ELRTAKDHLLSLIDVQTDRLHIIKLDPRQPVLHIGKHRDFEQNMGSFFIH"
     gene            complement(1097..1840)
                     /locus_tag="PLY05_08025"
     CDS             complement(1097..1840)
                     /locus_tag="PLY05_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03288.1"
                     /translation="MSLFGDLFDFVVDTVVDVVETAGTIVVDTVELAGTVATGAIEVA
                     ADVVETTSSIAADTLDFTGGILADAMDVTVEVASDLVDGIGSMASDSLDLTGELVAGL
                     IGVILGNGNSNEEREQAERRYERQLSALREKSDTAIVQYKQKTQQTYNQRLASAIEKQ
                     HTQRQALLSKALTDLNQERRFVRDCIGQLKTHKDRLNSQLTICFKTHQQEILKQEIKA
                     VNQLLSPLYGRLKQINSHCDELTISAYKP"
     gene            complement(2047..2526)
                     /locus_tag="PLY05_08030"
     CDS             complement(2047..2526)
                     /locus_tag="PLY05_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03289.1"
                     /translation="MANLPLITVVADVVGNLINGHYRHQQQMTQLENEYRLEKARIEQ
                     DTTLSCKKIDALLSYALKDLEVKVEHHRQHFSLLVNSLNLQLEESRVLQQHINDLMKV
                     AINPQEVPEIHIMVLKILDTMLEAQRQNSTQRHNLLVQHLQNSMLIDSNLGGFLPKL"
     gene            complement(2549..2938)
                     /locus_tag="PLY05_08035"
     CDS             complement(2549..2938)
                     /locus_tag="PLY05_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03290.1"
                     /translation="MATWPIASLEELSKQGYLVRLSTYLLQQQALQKNKSWQHKLMKQ
                     QASFLVDYDLLFRRITHLLVTQGYDFSSLHPHQTLKKLLLLLDSNAFSEAELSHLVEC
                     RHNVKYGFMDKATPQAVAILADLRVGV"
     gene            complement(2941..3492)
                     /locus_tag="PLY05_08040"
     CDS             complement(2941..3492)
                     /locus_tag="PLY05_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC03291.1"
                     /translation="MMTSFSQPLNTNWVYVSEQGFSALKQQLETTQARYAEVCEQRRI
                     AHELSGDGWHDNPEFNRQQQMEAFLNGELKRLLENFQKAKIIYIQEGARPTDAVSIGS
                     VVEVEITNVFSGNSHLEVWEIVGHGESNAELKKLAYDVPLARAMMNLQIDDCSDEFEL
                     RGQEVFVSINKLFASRQQAGLGD"
BASE COUNT         1045 a          748 c          684 g         1106 t
ORIGIN      
        1 gtggaacata ataaaaacta ataaaaagca ggttttaata gtttttggct attttgttaa
       61 agaacataaa actgacgcat ttaaccgcac cagttttatc ataggggcgt tcccaagact
      121 ttaattataa ccaaattaaa aaaattttct agttaaaaaa tagccattaa cagcgtacat
      181 ccgtgtactg gtatggctaa ccaatcctaa ttgcggccat caaaatttaa acttgcaggc
      241 aaaccataag ccagtgtctc aaaataacag gctgctgctg ccccctccaa tccgcgtacc
      301 gtgtcaatgt tgctggcttg tttaaggggt ttaataacaa tgccatctaa acgtttaatg
      361 gcattgtaaa attgtgattt atgttggggg tggcagcgtt ccatctcttg taataattgc
      421 cgatggcctt gtattttttg ggctaccagt tgtttggcaa aggccacaca taataaatct
      481 tgttgactca gggcatattg tgcctgacga cgttgagcat ctagtccata acagggcatc
      541 aaaagggtgg gttcggcttt tagaccatac agcaccacta caccaatctt atactcaccc
      601 aacttggcca agacactggc actcagctgc acatcacctt gaataaaaat acgttttagc
      661 ggggaaaggg gaacggtatt aatacgtgtt ttatcttgat aaacaagcaa ggcattatta
      721 tcaactttga gttctatacc acgtttatca acaaataaag tattcatggt ggttctcgtt
      781 gttgttttat gggaagggcg gtttaatgaa taaaaaacga ccccatgttt tgctcaaaat
      841 cacgatgttt gccaatgtgt aatacaggct ggcgtgggtc taatttaata atgtgtaagc
      901 ggtctgtttg cacatcaatc aggcttaaca aatggtcttt ggcggtgcgt aattcattgg
      961 cggtaaacca acattccatc gccgattttt gtccttgcac gcgccagcct tgcaaatact
     1021 gacgcacttg ttgcaaggtg tcctcgtcgc taatgtcata acacaccaaa tataaatcac
     1081 ggctcatgtg gggctcctaa ggtttatatg cactgattgt taattcatca caatggctat
     1141 tgatttgttt aagtctgcca taaagggggc ttaataattg attaaccgct ttgatttctt
     1201 gttttagaat ctcttgttgg tgtgtcttaa aacatatagt taactggcta tttaaacggt
     1261 ctttgtgtgt ttttaattgg ccaatacagt cacgcacaaa acgacgctct tggttaagat
     1321 cggttaatgc tttgcttaat agtgcttgac gttgcgtatg ttgtttttct atggctgatg
     1381 caagacgttg attgtaagtc tgctgtgttt tttgtttgta ttgcacaatc gcagtgtcac
     1441 ttttttcacg caaggcagat aattgacgct cataacgacg ttcggcttgt tcgcgctctt
     1501 cgttagaatt gccattgcct aaaataacac caattaagcc tgcaactaat tcgcctgtta
     1561 gatctaggct atcactagcc atactaccaa tgccatcaac taaatcactg gcgacctcaa
     1621 cggtaacgtc catggcatca gcaagaattc cgccagtaaa atcaagcgta tctgcagcaa
     1681 tgctactggt tgtttcaacc acatcagctg caacctcaat cgcgcctgtc gccacagttc
     1741 ctgccaattc aacggtatca acaacaatag ttcctgctgt ttccaccaca tcaaccacgg
     1801 tatctacaac aaaatcaaac aaatcaccaa ataaagacat tatcgttacc tcaaggcttg
     1861 cgttgcaaaa atgaatgagc gaatcaaaac agtggggtga catccttgtc ggctctcctt
     1921 gcttaagaaa gcaaccagat aatggcaata atcatagcca cctcctattt gttgttaatg
     1981 acgtttgctt gttggttatt ttatggtgtc attgcttaaa tgacgcaatt cgcaagctta
     2041 tgtcgtttat aatttgggta aaaaaccacc caagtttgag tctataagca ttgagttttg
     2101 taaatgttga accaataagt tatggcgttg tgtggagttt tgtcgttgag cttctagcat
     2161 ggtatctaaa atttttaaaa ccattatgtg aatttctggc acttcttgag ggttgatggc
     2221 cactttcatc aagtcattaa tgtgttgttg taatactcta ctttcttcta actgtaaatt
     2281 taagctgtta actaataagg aaaagtgttg acgatgatgt tctactttga cttctaaatc
     2341 ttttaaggca taacttaaaa gggcatcaat ttttttgcag gaaagtgtag tatcttgctc
     2401 aatacgtgct ttttctaaac ggtactcatt ttcaagctga gtcatttgtt gttgatgacg
     2461 ataatgacca ttgattaaat tgccaaccac atcagcgact acggtaataa gtggtaaatt
     2521 agccatgata agtacctcta taaaaacatt aaacgcctac gcgtaaatcc gccaaaatcg
     2581 ccacggcttg tggggtcgct ttatccataa agccatattt aacgttatgc cgacactcca
     2641 ccaaatgact taattccgcc tcgctaaacg cattgctatc taacagcaac aacagttttt
     2701 tgagcgtttg gtgagggtgc aaactcgaaa agtcataacc ctgcgtcact aataaatggg
     2761 taatgcgtcg gaataacaaa tcatagtcca ccaaaaaact tgcttgttgt ttcataagct
     2821 tgtgttgcca tgacttattt ttttgcaggg cttgttgttg caataaataa gtgcttaaac
     2881 gaactaaata accttgtttg cttaattctt ctaagctagc aataggccat gttgccatga
     2941 ttagtctcct aaacctgctt gttggcgtga agcaaagagt ttgttaatgc tgacaaagac
     3001 ttcttgacca cgtagctcaa actcgtccga acaatcatct atttgaagat tcatcatggc
     3061 acgcgccaaa ggaacgtcat aagccagttt ttttaattcg gcattagact caccatgacc
     3121 gacaatttcc catacttcca agtggctatt accagagaag acgttggtga tttccacctc
     3181 aaccacagac ccaatactca cggcatcggt tggacgtgcg ccttcctgaa tataaattat
     3241 tttggctttt tggaagtttt ctaacaaacg ctttaactcg ccatttaaaa aggcttccat
     3301 ttgctgttga cgattaaact cagggttatc gtgccaacca tcaccactta actcatgggc
     3361 aatgcggcgt tgctcacaca cttcggcata acgtgcctgc gttgtttcta attgctgttt
     3421 taaggccgaa aaaccttgtt cactcacata aacccaattt gtatttaatg gttgagaaaa
     3481 agatgtcatc ataaaaaact ccaaaataat taagcagctt taggccaaat agcggcacta
     3541 tacgcacata aaaatcgacc caataaacag gctaattcat ggc
//
LOCUS       DAOQMB010000235         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13604_length_3582_cov_5.345336, whole genome shotgun sequence.
ACCESSION   DAOQMB010000235 DAOQMB010000000
VERSION     DAOQMB010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13604_length_3582_cov_5.345336"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2738
                     /locus_tag="PLY05_08045"
     CDS             <1..2738
                     /locus_tag="PLY05_08045"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNC03292.1"
                     /translation="NFRSAPPIVRFSNVVQVLRALLFKLNSLCPQIAWGQSTANYSPV
                     MKFSIHDKDVWQSIKKFKDIVFIVPCAENEETDYIQKHPFLKTYIQVNEDGSTSLPVL
                     SASRAKGLEFNRVVVLDFASHIPHKLSDILARYKDQDVNMESDLALQYFLNRLYVAVT
                     RAKRQLIILDTDHSLQDFWHYFTDPNNLLPLHYLRQSKEVWQSQLGVIADMPLSAITD
                     ADNNEDNALDNAREFARNGRDQKDAYLLRQAGSLYRQVDEHLDSLLCFAEAYQLDKQF
                     SQAAKYFIQANSFDEAFLCYWRGQKATWADLLNVASKKTTLEARLEFRFASLYRNTRA
                     SEQEIAKLFTVLSKNSKQVILEEDEDLKAWQYVCEHLLNRLVKTKNSQSWHEVSKALN
                     LICQQQPLFKSIILLRHRAEIAFLAKEYQLARQLWEESKENKPNNYYIAVAETEPFPA
                     NIAALQKLKRFSTIIEQFENYVHKEQLQAQDWSVIGNTLCKEEAFDKLINIMPKLRHN
                     LELIKEIRRSAERKKKPLLVMRCRAAEIVDWIQTEQWDELLKYWRDKVDEKQKTFIIP
                     VIVRSLARSDGYLKLSDAKLEGDDLKKREQLQEIVRQQYLGSKEDKTPLFKVPEELIF
                     EVGAAFERARRFVDILNYYEAMIQRFPQHETELAKRWIVCKERQVKYYENIAKRSDDP
                     NEREKSKQIAEDNNNRATRARHKLDLSVTDDLGDFPVLSTLQELMIECLTEAEKTLVL
                     APTINTSPKKIEQELELVPEIPVDTISATSEVEAVVQQAEVEILAVTQAKSDNLEQPT
                     EETGITNKTFSAQPRAPKTDHKVKENIKLELGEYGIHIIRKNQRINISDEEKGETFSI
                     KSAGEELTGDWMMEHQEDFILLKGTPLWLHITQHEQQKIIKIGHSVLGLILDIAV"
     gene            2743..3060
                     /locus_tag="PLY05_08050"
     CDS             2743..3060
                     /locus_tag="PLY05_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03293.1"
                     /translation="MPIAKSKMYLYLPKYAERARYLSPLSMVANFNIYFYCLLGSQGK
                     EHIEAAQKHTKIKIVAGVESSKEARQALAQAYPNIMLFNDILALKQASTTLGLQGLIL
                     AFI"
BASE COUNT         1211 a          609 c          714 g         1048 t
ORIGIN      
        1 ataacttccg ctctgcacct ccgattgtta gatttagtaa tgtagttcaa gtattgcgtg
       61 cgttattatt taaactaaac agcctatgcc cacaaattgc atggggacaa tctacggcta
      121 actatagccc tgtcatgaag ttttcgattc atgataaaga tgtttggcaa agtatcaaaa
      181 aatttaaaga tattgtgttt attgtgcctt gtgccgaaaa cgaagaaacc gactatattc
      241 aaaagcatcc ttttttaaag acatatattc aagttaatga agatggttca acctctttgc
      301 ctgtattaag tgcatcacga gcaaaaggtt tagaatttaa tcgtgttgta gtattggatt
      361 ttgctagtca tatccctcat aagttgtctg atatcttagc tcgttataaa gaccaagatg
      421 ttaatatgga aagcgacctt gccttgcaat actttttaaa tcgtttgtat gtggctgtta
      481 ctagagcaaa acgacaattg attattttgg ataccgatca tagcttacaa gatttttggc
      541 attattttac cgatcctaat aatttattac ctttgcatta tttacgtcag tctaaagagg
      601 tttggcaaag ccaattaggg gttattgctg atatgcccct aagtgctatt actgatgctg
      661 acaataatga agacaatgcg cttgataatg ctagagaatt tgcgcgtaat ggtcgcgatc
      721 aaaaagatgc ttacttgtta cgtcaagcag gtagtttata tcgacaagtt gatgaacatt
      781 tggatagttt attatgtttt gccgaggctt accaattaga taagcaattc tctcaagcgg
      841 cgaaatactt tattcaggca aatagttttg acgaagcttt tttgtgttac tggcgcggtc
      901 aaaaagcgac ttgggcggat ttgttaaatg ttgctagcaa aaaaacaaca ctagaagcgc
      961 gtttagagtt tcgttttgct agtttgtatc gtaatactag agcgagtgaa caagaaatag
     1021 ccaaattatt tacggtgcta agcaaaaata gcaaacaagt gatactagaa gaggatgaag
     1081 atttaaaggc atggcaatat gtttgtgaac atttattaaa tagattggtc aagacaaaaa
     1141 actctcaatc ttggcatgag gtttctaagg ctttaaatct tatttgccaa caacagccgc
     1201 tgtttaaatc gattatattg ttacgacatc gtgcagagat agctttccta gcaaaagagt
     1261 accaattagc tcgccaacta tgggaagagt ccaaagaaaa caaacccaat aattattata
     1321 ttgctgttgc tgaaacagag ccatttccag cgaatattgc agcactacaa aaactaaaac
     1381 gcttttcaac gattattgag caatttgaaa attacgttca taaggaacaa ttacaagctc
     1441 aagactggtc tgttattgga aatacgttat gcaaagaaga agcttttgac aaacttatca
     1501 atattatgcc caagttgcgt cataatcttg aactgattaa agagattagg cgtagtgctg
     1561 aaagaaagaa aaaacctctc ttagtaatgc gctgtcgtgc cgctgaaatt gtggactgga
     1621 tacaaacaga acaatgggac gaattactca aatattggcg tgacaaagta gatgaaaagc
     1681 aaaaaacatt tattattcca gtgattgtac gaagtttggc tcgttctgat ggctatttaa
     1741 aattatctga tgctaaatta gaaggggatg acctgaagaa acgtgagcaa ttacaagaga
     1801 ttgttcgtca acagtattta ggctcaaaag aagataaaac ccccttattt aaagtaccag
     1861 aagaattaat atttgaagtg ggtgccgcat ttgaacgagc acgtcgattt gttgatattt
     1921 tgaattatta tgaggccatg atacagcgtt ttcctcaaca tgaaactgaa ttggctaaac
     1981 gttggattgt gtgcaaagag cgtcaggtta aatattatga aaatatagct aaaagaagtg
     2041 acgatcctaa tgaacgtgaa aaaagtaaac aaattgctga ggacaataat aatcgtgcca
     2101 ctagagcaag gcataaactt gatttaagtg tgacagatga cttaggcgat tttcctgtat
     2161 tgtcaacatt gcaagagctg atgattgagt gtttaacaga ggcagaaaaa accttagtat
     2221 tggctcctac tatcaataca tcacctaaaa agatagaaca agagcttgaa ttagtgcctg
     2281 aaataccagt tgataccata tctgccacat cagaagttga agctgttgtt cagcaagcag
     2341 aggtagagat attagcagtc actcaagcca aatctgacaa ccttgagcaa ccaaccgaag
     2401 agacaggcat cacaaacaaa acgttttcag cccagcctcg tgccccaaaa actgaccata
     2461 aagtcaaaga aaatattaag ctagagttag gtgaatatgg catacatatt attcgtaaaa
     2521 atcagcgtat taatattagt gatgaagaaa aaggtgaaac tttttcaatt aagtcagcag
     2581 gagaggaatt aacgggtgat tggatgatgg agcatcagga agattttatt ttattaaaag
     2641 gaacacctct atggctacat attactcagc atgaacagca aaaaattatc aaaattggcc
     2701 atagtgtttt aggtctaatt ttagacattg ctgtttaagt ttgtgccaat cgctaaaagt
     2761 aaaatgtatt tgtatttgcc caaatatgct gagcgagcta gatatttaag cccgctcagt
     2821 atggttgcta atttcaatat ctatttttat tgtcttctgg gttcacaggg taaagaacat
     2881 attgaggcag ctcaaaagca taccaaaata aaaattgttg caggggtaga gtcatcaaaa
     2941 gaggccagac aagcccttgc tcaagcgtat cctaacatta tgctatttaa tgatatattg
     3001 gctctcaaac aagcaagcac aacattaggt ttacaaggct taattttagc cttcatttga
     3061 agtttaatcc tagcaaaaaa tcttaatctt tacttaggca cagattgtaa tggtcttaat
     3121 cccataaagt ctgggaaggt gttcaaacga cattcacatt ctatctacca ccttgggcat
     3181 tgtgtcttaa tcccataaag tctgggaaga ggttcgaact tacgttgcta cgcgagtcat
     3241 ttattatcaa ggtcttaatc ccataaagtc tgggaagagg ttcgaaccac tttaactaag
     3301 aggaaactaa aaatgactac agtcttaatc ccataaagtc tgggaagagg ttcgaaccta
     3361 tgccttcgat gccgaaggta atcgcttcac gtcttaatcc cataaagtct gggaagaggt
     3421 tcgaactaaa cggctatgta gagaccttaa tcccaaccgt cttaatccca taaagtctgg
     3481 gaagaggttc gaactgtata cactgattag aaatgcctta aaagagtctt aatcccatag
     3541 agtctgggaa gaggttcgaa ctgttaaagc aggtaacact gc
//
LOCUS       DAOQMB010000236         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13770_length_3554_cov_15.838239, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000236 DAOQMB010000000
VERSION     DAOQMB010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_3554_cov_15.838239"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(203..1045)
                     /locus_tag="PLY05_08055"
     CDS             complement(203..1045)
                     /locus_tag="PLY05_08055"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HNC03294.1"
                     /translation="MKQYLELMRHIKDNGTFKGDRTGTGTYSVFGYQMRFNLAEGFPL
                     VTTKKVHLKSIIHELLWFLSGDTNVKYLQDNGVSIWNEWATAEQCARFGRSEGELGPV
                     YGHQWRNFGATKNADGSFSPDGADQIAALVHDLKHNPNSRRLIVSGWNPQEANQVALP
                     PCHTLFQFYVANGKLSCQLYQRSADVFLGVPFNIASYALLTMMLAQVCDLQLGEFIWT
                     GGDCHLYSNHLEQTELQLSREPLPLPTMRLNPNVKDIFAFKYDDFTLENYQSHAVIKA
                     PVAV"
     gene            complement(1057..1854)
                     /gene="lgt"
                     /locus_tag="PLY05_08060"
     CDS             complement(1057..1854)
                     /gene="lgt"
                     /locus_tag="PLY05_08060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNC03295.1"
                     /translation="MLTYPQIDPVAISLGPLQVHWYGLMYLCAFGMAYFLGTLRTKRM
                     NPVWSADQLSDLIFYAAMGVILGGRCGYVFFYSFDQFLANPLWLFQVWKGGMSFHGGF
                     LGVMAAMLFFAKRYKLGFWQVMDFVAPLVPTGILFGRLGNFIGGELWGRPVVDPNYPL
                     AMVFPHVDNLPRHPSQLYEAFLEGALLFAILWWFSSKPRPVKAVSGVFALGYGLARFT
                     VEFFREPDADKGFIAFNWLTMGQLLSTPLIFIGILLLVLAYRQPAKV"
     gene            1929..2696
                     /locus_tag="PLY05_08065"
     CDS             1929..2696
                     /locus_tag="PLY05_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HNC03296.1"
                     /translation="MCLLAFAWQVHPNYPLILIGNRDEFYARPAQAIDYWQDAPFILA
                     GRDEQEGGTWLGVTKDGRWAALTNYREPNAPKGSLSRGHLVAEFLKADSEPLAYAQQV
                     FTQAEQYSGFNLLVGNKQQVAVVSNRGTEPQLLNQGIYGLSNQLLDSPWPKSQRLKAG
                     LSQLMQHDHFDLQQALLLLQDQTQPDDADLPNTGVGLVWERLLATAFIKSPVYGTRAS
                     SILLMSNDNIQLTEQTWLSGEQGELKQLGFSINTFIL"
     gene            complement(3121..>3554)
                     /locus_tag="PLY05_08070"
     CDS             complement(3121..>3554)
                     /locus_tag="PLY05_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03297.1"
                     /translation="IDIAATLNEAQKLLAQLSQGILVILVFVVLAGCLVLIASLAASF
                     DSRLHEAALLRALGAKRQQLQWRTGIELAILGTLAGLLAVILTEIITALISVFLLEGK
                     AHIHAWLWLTPLASASLVLVIGLWHLRKTWQVSPMVVLKEV"
BASE COUNT         1126 a          735 c          726 g          967 t
ORIGIN      
        1 cgggtaaata ccacggcaat ttgttatcaa tgccaataca gccattttcg gcaactgcaa
       61 caattaaaga gagtttcatc gttaatatat tctcagaaaa ctcctccacc tgataagggg
      121 gaggttggaa ggggattagt ttgtactgtg taaaacctca ccctaaccct ctgcttaaaa
      181 aggagagaga acttttctag ctttatactg ccacaggcgc tttaatgacc gcatgggatt
      241 gataattttc tagcgtaaaa tcgtcatatt taaaggcaaa aatatcttta acatttgggt
      301 ttaaacgcat ggtcggtaag ggcaacggtt cacgactaag ttgtaattca gtttgctcga
      361 ggtgattgct atataaatgg caatcaccac ctgtccaaat aaactcacct aattgcaaat
      421 cacagacttg tgccaacatc atggttaata aagcataaga tgcaatatta aacggcacgc
      481 ctaaaaacac atccgcacta cgttgatata attggcagga taatttgccg ttagccacat
      541 aaaattggaa taaggtgtga caaggtggca acgccacttg attagcttct tgcggattcc
      601 aaccagagac aattaaacgg cgagagtttg gattatgttt gaggtcatgg acgagggcgg
      661 ctatttgatc agcaccatca gggctaaaac taccatcagc gttttttgtt gcgccaaaat
      721 tacgccattg atggccatag acaggcccta attcgccttc actacggcca aaacgtgcgc
      781 attgttcggc agttgcccat tcgttccaaa tactaacacc gttatcttgc aagtatttaa
      841 cgttggtatc accacttaaa aaccataata attcgtgaat aatcgactta agatgtactt
      901 ttttagtggt cactaaggga aagccttccg ccaagttaaa gcgcatttga tagccaaata
      961 ccgagtacgt gcctgtgcct gtgcggtcac ctttgaatgt gccattgtct ttgatatgac
     1021 gcattaactc taaatattgt ttcatagcgg atcacctcaa actttagcag gctgacggta
     1081 tgccaatacc aataataaaa taccaataaa aatcaatggg gtgctgagca gttgtcccat
     1141 cgttaaccaa ttaaaggcaa taaaaccttt gtcagcatca ggttctctaa aaaattcgac
     1201 agtaaatcgt gctaagccat aacccaaagc aaatacgcct gatactgctt tgacggggcg
     1261 aggtttgctg ctaaaccacc ataaaatggc aaataataaa gccccctcta aaaaggcttc
     1321 atatagttgc gaagggtggc gtggcaggtt gtccacatga ggaaatacca ttgctaaagg
     1381 ataattgggg tcaacaacag gacgccccca taattcccca ccaataaaat tacctaaacg
     1441 accaaataaa atacctgttg gtacaagcgg tgcaacaaaa tccatcactt gccaaaagcc
     1501 taatttatag cgtttggcaa aaaataacat agcagccatt acgcccaaaa agccaccatg
     1561 aaaactcatg ccacctttcc acacttgaaa aagccacaaa gggtttgcta aaaactgatc
     1621 aaagctataa aagaagacgt aaccacaacg tccgcctaaa ataacaccca tcgcagcata
     1681 aaaaatcagg tcagataatt gatcggcaga ccaaacgggg ttcatgcgtt tggtgcgtaa
     1741 agtgcctaaa aaataggcca tgccaaaggc acataaatac attagaccat accaatggac
     1801 ttgaagtggc cctaaactaa tggcaacagg gtctatttgt ggatatgtca gcataaatga
     1861 tgtttccaac aagatttaac taaaaatttt tctattttat cttaaatttt aaaaatgagg
     1921 gtgtagttat gtgtttatta gcgtttgctt ggcaagttca tcccaattac cccttaattt
     1981 taattggcaa ccgtgatgag ttttatgcgc gccctgctca agcgattgat tattggcaag
     2041 atgccccatt tattttggcg ggtcgagatg agcaagaggg tggaacgtgg ctaggtgtga
     2101 ccaaagatgg gcgttgggcg gcattaacca attatcgtga acccaatgca ccaaaaggct
     2161 cgttatcacg cggtcatttg gtggcagagt ttttaaaggc agatagtgag cctttggctt
     2221 atgcccaaca agtatttacc caagctgagc agtatagtgg ctttaatttg ttggtgggta
     2281 ataaacaaca agtagccgta gtcagtaatc gaggtacaga gccacagtta ttaaaccaag
     2341 ggatttatgg tttaagtaat cagttattag actcaccgtg gccgaaatct cagcgtttaa
     2401 aagcaggttt aagccaatta atgcaacacg atcattttga tttgcagcag gctttgttgt
     2461 tgttacaaga tcaaacgcaa cctgatgatg ctgacttgcc aaatacgggg gtgggtttgg
     2521 tgtgggaacg tttgttagca acggcgttta ttaagtcacc tgtgtatggc acacgtgcct
     2581 caagtatctt gttaatgagc aacgataaca ttcaattaac cgagcaaaca tggttgagtg
     2641 gagaacaagg ggagttaaag caattaggtt ttagtatcaa tacattcata ctgtaatggt
     2701 ttaatagagc aggacagtaa ctcaatggct tcaggttgaa taggtatagt caaagaatct
     2761 gaaaactgct acatgattca tctgaaaagt cctgtaattt aaagcgtttt tacgatgctc
     2821 tttgtggttg ttttaatttt ctttcacttt ggtattactc aaaaaaagac cttaattcac
     2881 cctcaagcta accctgaaaa atgccaagag tttattgagc agctagaggc ttacgaaacc
     2941 aacaaacgac cgattattta ccttgatgaa agtggcttta aggcacatga tcacagggtt
     3001 tatgtgtatc gaaaatattg cctctagcaa atattaccat gcaggtggtt catcaaagcg
     3061 gtctttggtg caattgcccc agttagaccg cacgattact ttgggttttg aagtggattt
     3121 ttagacttct tttaacacca ccattggcga cacttgccat gttttgcgta aatgccataa
     3181 gccaatcact aataccaaac ttgcactggc taacggtgta agccataacc aagcatgaat
     3241 atgcgctttg ccttctaata aaaatacgct gattaaggca gtaataatct ctgttaaaat
     3301 gacggctaat aaacctgcca atgtgcctaa aatcgcgagt tcaatacctg tacgccattg
     3361 cagttgttga cgttttgctc ccaatgctcg taacaaggca gcctcatgca agcgactatc
     3421 aaaactagcc gctaaactgg caattaacac taaacagcct gccaacacca caaacaccaa
     3481 aatcaccaaa ataccttggc tgagttgtgc caacagtttt tgtgcttcat taagtgtggc
     3541 ggcaatatca ataa
//
LOCUS       DAOQMB010000237         3548 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13809_length_3548_cov_10.577727, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000237 DAOQMB010000000
VERSION     DAOQMB010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13809_length_3548_cov_10.577727"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..704)
                     /locus_tag="PLY05_08075"
     CDS             complement(66..704)
                     /locus_tag="PLY05_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC03298.1"
                     /translation="MTRILLVDDHTLFRSGIRALLASQEDIDVVGEAGEGSEALKLCQ
                     ILKPDIVLLDLNMPGLSGLEVLKLMLEDSHQQLVIMLTLSEEAEDLLKALQLGAKGYL
                     LKNSNVDYLVNAIRLVAQGGTAIQPEMTSTLVAGLRQNKKEETEEKEQLTLREKEVLK
                     LVSSGQSNKEIARKLDIGESTVKFHIQSILRKLNLSSRVQAAVYASQHPHIS"
     gene            complement(713..2653)
                     /locus_tag="PLY05_08080"
     CDS             complement(713..2653)
                     /locus_tag="PLY05_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNC03299.1"
                     /translation="MPTIINNLKTRLTTKITAMLLAALCVALLAIGYTLWLSWQLEGA
                     AAAINDSGSLRMRAWKIAALLNQTNQDRSFTPQQLAEVKTIQQQFEITLAELRKGDPQ
                     RPLFLPRQRQIQQQLTILERTWQQQFVPFLSNVQQYKHLNPQQAQIWLTQTNLFVEQI
                     YQTVNLIEEEYARQTFWLRSSQMMLIFMAFIGTLVMIILLNLMIIRPVNSLHQGIQQM
                     YNGDFSVRLTIEGEDEFAQLSAGFNAMANQLQQLYNNLEQRVNEKTESIAKKNRYLSV
                     LYDFSQKLNQHHSLEELSRHFLQRLMLEFNATGGSVRLIDKTANKIYSVVDEGIPPSL
                     SQQMQCQQLGSCFCTQAAEQAKTAINNLNQHFSPHFSHCLAAGFKMVLAIPIQTADKN
                     QGIFNLHFSEEREISAEEILLLETLGQQLGIAIDNQRLTGKEKELAVFEERNLVAQGL
                     HDSIVQSLSFLNIQLQLLQTAVKQQNNQQINTIIPLLQTGVQQGYEDLRELMSNFRSR
                     YDDSQLEVAINAIIRRFSEQTNIPVTLKLDNLGKPLANNERLQILFIIQEALSNIRKH
                     AQAKHININIINHADVDILINDDGIGFNHQEVQSSHHQHIGLKIMAERAKRINAQLDI
                     ESTPMIGTTIKLHLSQNQRQNA"
     gene            2837..>3548
                     /locus_tag="PLY05_08085"
     CDS             2837..>3548
                     /locus_tag="PLY05_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC03300.1"
                     /translation="MVSENKQKYSVLIVSTLAFTVCFMTWMMFGVIGIPIKKMLSLNA
                     TEFGLLTSTPILTGALSRIFLGAWTDKYGGRIVLFILMLSCVIPIYLISYATEYWQFL
                     VLGLFVGLAGGSFSVGTPYVARWFEKSQQGFAMGVFGAGNSGAAVNKFIAPVLVVSFG
                     WAFVPQVYAAILLVTAILFWFFSYSNPNHLVGKSVTLREQLVVLKDPRVWKYCQFYSI
                     VFGGYVALALWMVQYYVGE"
BASE COUNT          968 a          593 c          719 g         1268 t
ORIGIN      
        1 ttttgtctgc tggtgttgat ggcattaaag tggctgaagc cgttgctttg agtataaccc
       61 aaaatttatg atatatgcgg atgttgactc gcataaacag cggcttgcac acgactactt
      121 aaattaagtt ttcgtaaaat actttgtata tgaaacttaa ctgtactttc accaatatca
      181 agtttacgag caatttcctt attactttgt cctgacgaaa caagttttaa aacttctttt
      241 tcgcgtagag tgagttgctc tttttcttca gtctcttctt ttttgttttg tcgtaaacca
      301 gcaactaatg tagaggtcat ttcgggctga atggccgtgc ctccttgtgc gacaagtcgg
      361 atagcgttga caagatagtc aacattgcta tttttgagta aatagccttt tgcccctaat
      421 tgcaaagctt ttaataagtc ttcggcttct tcagagagag taagcataat taccagttgt
      481 tgatggctat cttctaacat gagttttagc acttctaaac ctgataaacc tggcatattt
      541 aagtctaata aaacaatatc gggtttcagt atttgacaaa gtttaagtgc ttcagaacct
      601 tcgccagctt caccaacgac atcaatatcc tcttggcttg ctaacaatgc tcgaatgcca
      661 cttctaaata aagtatggtc atcgactaat aaaatacgtg tcataagaac tcctaagcat
      721 tttgtcgttg attttggctt agatgaagtt taattgttgt gccaatcatg ggagtagatt
      781 caatgtctaa ttgagcatta atgcgttttg ctcgctcagc cataattttt aaaccaatat
      841 gttgatgatg gctagattgt acttcttgat gattaaagcc aataccatca tcattgatta
      901 aaatatcaac atcggcatga ttgataatgt tgatattgat gtgtttagct tgtgcatgtt
      961 tgcgaatatt tgataaggct tcttgaataa taaacaggat ttgtagccgt tcgttgttag
     1021 ccaagggttt acctaagtta tctaatttaa gtgttacggg aatattggtt tgttcactaa
     1081 agcgtcgaat aatggcatta atagccactt caagttgact atcatcataa cgactacgaa
     1141 agttgctcat tagctcgcgt aagtcttcat aaccttgttg tacacctgtt tgcagtagag
     1201 gaatgatagt attaatttgt tgattgtttt gctgtttaac tgctgtttgt aataactgaa
     1261 gttgtatgtt caggaaactt aaactttgta caatactatc gtgtaagcct tgagcaacca
     1321 aattacgctc ttcaaaaacg gcaagttctt tttctttgcc tgttaatctt tgattatcaa
     1381 tcgctattcc taattgttgt cctagggttt ctaataacaa aatttcttct gcactaattt
     1441 cgcgctcttc actaaaatgc aaattgaaaa taccttgatt tttatctgcc gtttgaattg
     1501 gaattgctaa aaccattttg aagcctgcgg ctaaacaatg ggagaaatgt ggagaaaagt
     1561 gttgattaag attgttaatc gcagttttgg cttgttcggc tgcttgggta caaaaacagc
     1621 tacctaattg ttggcattgc atttgttgtg ataaagaggg tggaatgcct tcatcaacaa
     1681 cgctgtaaat tttattggcc gttttatcaa ttaaacgtac gctaccccct gtggcattaa
     1741 actcaagcat taaacgttgc aaaaaatgtc gagataattc ttctaaagaa tggtgttggt
     1801 tgagtttttg gctaaaatca taaagtacag ataaataacg attttttttg gctatacttt
     1861 cggttttttc attaacacgc tgttctagat tgttgtaaag ttgctgtaat tgattagcca
     1921 ttgcattaaa accagcactt aattgggcaa attcatcttc tccttcgatg gttaaacgta
     1981 ccgaaaaatc tccgttatac atttgttgta tgccttgatg taggctattg acgggtctaa
     2041 taatcattaa atttaataaa ataatcatga ctaatgtgcc aataaaagcc ataaaaatca
     2101 acatcatctg tgatgaacgt aaccaaaaag tttggcgagc atattcttct tcgataaggt
     2161 taaccgtttg ataaatttgt tctacaaaca gatttgtttg tgttaaccaa atttgggctt
     2221 gttgtgggtt taaatgtttg tattgttgta cattacttaa aaagggaaca aattgctgtt
     2281 gccatgttcg ttctaatatc gtgagttgtt gttgaatttg ccgttgcctt ggtaaaaata
     2341 aaggtcgttg ggggtcgcct ttgcgtaatt ccgccagtgt aatttcaaat tgttgttgaa
     2401 tagttttgac ttcagctaat tgttgtggcg taaaagatcg gtcctgattt gtttgattaa
     2461 gtaaggcggc tattttccat gctctcattc ttaaactgcc actgtcatta atggctgccg
     2521 ccgcaccctc taattgccat gataaccata aggtataacc aattgctaat aaagcaacac
     2581 ataatgcagc caataacatg gctgtgattt tggtggttaa tcgtgtttta agattgttaa
     2641 taattgttgg cataaaaaga aatattgtag gcagtttatt gtttattcca gcacagttta
     2701 gcttgcacta aacctagtct aaaggctagg tcttgcctct tcttttagat acacctcgct
     2761 atgcgtttga ccgataacct tacaagtgta atgggtttac tcttggtata gctcctaaaa
     2821 aggtggattg ctaaaaatgg tctcagaaaa caaacaaaaa tattcggtat taatagttag
     2881 cacattggct tttacggtgt gttttatgac atggatgatg tttggtgtga taggtattcc
     2941 tatcaaaaaa atgctcagtc tcaatgccac cgaatttggt ttgttaacat caacccctat
     3001 actgactggc gcattatcac ggatattttt gggggcatgg acggataaat atggcggtcg
     3061 tatcgtttta tttattttga tgctgagctg tgtcattcct atttatttaa ttagttatgc
     3121 cactgagtat tggcagttct tggtattggg gctatttgtt ggtttagcag gtggcagttt
     3181 ttcggtggga actccctatg tggctcgttg gtttgagaaa agtcaacaag gttttgcgat
     3241 gggggttttt ggtgcaggta actcaggagc tgcggttaac aaatttattg cccctgtgtt
     3301 ggtggtttct tttggttggg cttttgtgcc acaggtttat gccgccattt tattagttac
     3361 tgccattttg ttttggtttt ttagctatag caaccctaat catttagtgg gtaagtcggt
     3421 gacattgcgt gagcaattag tcgttcttaa agacccacgt gtttggaagt attgccaatt
     3481 ttattccatt gtttttggcg gttatgtcgc tttggcatta tggatggtgc agtactacgt
     3541 gggtgaat
//
LOCUS       DAOQMB010000238         3532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_13903_length_3532_cov_12.780270, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000238 DAOQMB010000000
VERSION     DAOQMB010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13903_length_3532_cov_12.780270"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLY05_08090"
     CDS             <1..285
                     /locus_tag="PLY05_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03301.1"
                     /translation="GGTTWQLDSGQRNSARRKPKKSKLNALKTGEYTADKVAERKRKA
                     KVKKSCGWGCSYGDKRFRWNRSINYCGFMKTTKKAEREKAKERVKAALKD"
     gene            complement(654..1145)
                     /locus_tag="PLY05_08095"
     CDS             complement(654..1145)
                     /locus_tag="PLY05_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC03302.1"
                     /translation="MLKKILVMTFIMANIANADEITIENNQLKFSQPLIFATQTEELL
                     PDNKVTVEQIKTFLEKKSYISTLRIEGHVNSFEDEQKNLLLSAERGLSVAKALVEAGV
                     DCQRLLISAFGSSKPIEANSTPEGRAANNRVDLFVAALRGKAIGGMPLEGVGQRVGDA
                     CAK"
     gene            complement(1175..2098)
                     /gene="ylqF"
                     /locus_tag="PLY05_08100"
     CDS             complement(1175..2098)
                     /gene="ylqF"
                     /locus_tag="PLY05_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HNC03303.1"
                     /translation="MSINWFPGHMNKAIREIKEILPKVDLVIEVLDARLPYSSSNPVI
                     AKFCSDKPSLKLLTKSDLADPIITEQWLAYFRQQSQTQALAITTEKPEQIRGLIGDIK
                     QMLAGKEDKLGNLTALITGIPNVGKSTLINTLAGKQIAKAANEPAVTQGQQRIDLRNG
                     LILIDSPGILWPKIENPKSGYRLALAGSIKNTAMNHEDVACFGAEFFLKQYPEFMKKR
                     FKLEDLPSHEVEFLEIIGKQRGCLGSGGYVDFHKIATILLLEFRSAILGRISLETPEV
                     AIAEQQEVERILAEKAAQKELEGQQKKKRKR"
     gene            complement(2202..>3532)
                     /locus_tag="PLY05_08105"
     CDS             complement(2202..>3532)
                     /locus_tag="PLY05_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNC03304.1"
                     /translation="AYEQVKNDPVMYAHANRVLHLETNPGNARALVQRHGEQEVWLNP
                     PPIPLSTEEMDYVFDLPYARVPHPVYGDAKIPAYEMIRFSVNIMRGCFGGCTFCSITE
                     HEGRIIQNRSEDSIIREIEKMRDVPGFTGVVSDLGGPTANMYRLACKSPKIEAACRKP
                     SCVYPDICENLNTDHSHLIQLYRRARQLKGVKKILIGSGLRYDLAVRSPEYVRELVTH
                     HVGGYLKIAPEHTEEGVLSKMMKPGIGAYDKFKQLFERFSKEAGKEQYLIPYFIAAHP
                     GTRDEDMMNLAIWLKKNGFRADQVQTFYPSPMATATAMYHSGKNPLKKVTRKSEAVEI
                     VKGERRRRLHKAFLRYHDPNNWPMLREALKEMGRADLIGNGKHHLIPNYQPQTDGTYQ
                     TARKKNSSTEQGVSQKVTAKKGRILTQHTGLPPREGVDKNKVKRPVKKRG"
BASE COUNT         1059 a          739 c          682 g         1052 t
ORIGIN      
        1 ggaggtacaa catggcagct agacagtgga cagaggaaca gcgcaaggcg caagccgaaa
       61 aaatcaaaac ttaatgcgct aaagactggc gagtacacag ccgacaaagt agctgaacga
      121 aaacgcaagg caaaagtaaa gaaaagttgt ggctgggggt gttcctatgg cgataagcgt
      181 tttagatgga ataggtcaat aaattattgt ggatttatga aaaccactaa aaaagctgaa
      241 agggagaaag ccaaagagcg agtaaaggca gcattaaagg attagggggt attatcttac
      301 aaagttatta agagttaatt tttaataacc tttggctatt agtaccgcca ccatgacaaa
      361 aacattttgt aatcgggtat ttttggctaa aaaatgcttt ttaggggggg gtcacttttc
      421 ttttttgggg cattataggg ggcatttttg ataaattaaa aaataaaaac tatataaaac
      481 aataagttaa attaactttg tgaagccctt actggcacca aatagacttc taagcaagtc
      541 attaccgtat aaaaagcctt gattttcaac gaatcagggc ttttttattg tctatagtat
      601 tctatggtag tcattgaaag aggcaaaaat attgggtact tttatgggta actttatttt
      661 gcgcaagcat cacccacacg ttggccaaca ccttccaaag gcatcccacc aatcgcttta
      721 ccgcgtaagg cagcaacaaa caagtcgaca cgattattag ctgctctgcc ttcaggcgtt
      781 gagttagctt caataggttt gctgctacca aaggcactaa ttaataaacg ttggcaatca
      841 acccctgctt cgactaaggc tttagccacc gataaaccac gctctgcact gagcaacaaa
      901 ttcttttgtt catcttcaaa actattaaca tggccttcaa tccgtaaagt tgaaatgtat
      961 gactttttct ctaaaaaggt tttaatttgt tcaacagtca ccttgttatc aggcaataat
     1021 tcttcggttt gtgtggcaaa tattaaaggt tggctaaact ttaattgatt gttttcaatg
     1081 gtaatttcat ctgcattggc aatattagcc atgatgaaag tcatcacaag tatttttttc
     1141 aacatgatct gtcctcccca atgtatctaa tcaattaacg tttacgtttc tttttttgtt
     1201 ggccttccaa ctctttttgt gcagcttttt cggccaaaat ccgctcgact tcttgctgtt
     1261 ccgcaatggc gacttcggga gtttctaggc taatacgacc taaaatggca cttctaaact
     1321 ctaataacaa aatagtggca attttatgaa aatcgacata accaccactg cccaaacaac
     1381 cgcgttgttt gccaataatt tctaaaaact ccacttcatg gctgggcaaa tcctctaatt
     1441 taaaacgttt tttcataaat tcgggatact gttttaaaaa gaactcagca ccaaaacaag
     1501 ccacatcttc atggttcatg gcagtatttt taattgaacc tgccaaggct aaacgataac
     1561 cactttttgg gttttcaatt tttggccaca aaatacctgg tgaatcaatc aaaattaaac
     1621 cattgcgcaa atcaatccgt tgttgacctt gagtcaccgc aggttcgtta gcggctttag
     1681 caatttgttt acctgctaaa gtattaatta aggtcgattt acccacgttg ggaatccctg
     1741 taattaaggc ggtcaaatta cctaacttat cctctttacc ggccaacatc tgtttaatgt
     1801 cgccaattaa gccacggatt tgctcaggct tttcggtagt aatcgccaac gcttgggttt
     1861 ggctttgttg tctaaaataa gccaaccatt gttcggtaat aatcggatcg gctaaatcac
     1921 ttttggtcaa taattttaaa ctgggtttgt cgctacaaaa tttagcaatc acaggattag
     1981 aactgctata gggtaaacga gcatctaaaa cttcaatcac caaatccact tttggtaaga
     2041 tttctttaat ttcacggatg gccttgttca tgtggccggg aaaccaatta atactcatat
     2101 ctttaaatca catagtctgt atagctacta ttgtaacggc caataacaca aaaaacagcg
     2161 ataaaatact tattgcaaaa cgtgcgaatt agccataaaa atcaacctcg ttttttcaca
     2221 ggtcgtttta ccttgttttt atccacacct tcacgcggtg gtaagcctgt atgttgggtt
     2281 aaaatgcggc cttttttcgc agtgactttt tgagaaaccc cttgttcagt agaagagttt
     2341 ttcttccgtg ctgtttgata cgtgccatct gtttgtggtt gataattagg aataagatga
     2401 tgtttaccat tgccaattaa atcagcacgt cccatttctt ttagggcttc gcgtaacatt
     2461 ggccaattat tcgggtcgtg ataacgtaaa aacgctttat gcaaacgacg acgacgctca
     2521 cctttgacaa tctccaccgc ttcgcttttg cgcgtcactt ttttgagtgg atttttaccc
     2581 gaatgataca tcgcagtagc cgttgccatt ggcgaagggt aaaacgtttg tacttggtcg
     2641 gctctaaagc catttttctt gagccaaatc gccaaattca tcatatcttc atcgcgtgtg
     2701 cctgggtggg cagcaataaa gtagggaatc aaatactgct ctttgcctgc ttctttagaa
     2761 aaacgttcaa agagttgttt aaatttgtcg tatgcaccaa tacccggttt catcattttt
     2821 gataaaacac cctcttcggt atgttcgggc gcaattttta aataaccacc gacatgatgc
     2881 gtgactaatt cacgcacata ttcaggcgaa cgcaccgcta ggtcataacg taaacctgaa
     2941 ccaatgagaa tttttttaac gcctttgagc tgccgcgcac gacgatacaa ttgaataaga
     3001 tgcgaatggt cagtattcag gttttcacaa atatcaggat acacacacga gggcttacga
     3061 caagccgctt caatcttggg tgatttacac gccaagcgat acatattggc ggtaggcccg
     3121 cccaagtcag aaactacacc cgtaaaaccg ggtacgtcac gcattttttc gatttcacga
     3181 ataatgctat cttcagaacg gttttgaata atccgacctt catgctcggt aatcgaacaa
     3241 aaagtacagc caccaaaaca gccacgcata atattgaccg agaaacgaat catctcatag
     3301 gcaggaattt tggcatcacc atacacagga tgtgggacac gtgcataggg caaatcaaag
     3361 acataatcca tttcttccgt gcttaacgga atcggcggtg gattaagcca tacttcttgc
     3421 tcaccgtgac gttgcactaa agctcgggca ttaccaggat tagtttctaa atgcagcaca
     3481 cgattagcat gagcatacat gacagggtca tttttgactt gttcataagc ag
//
LOCUS       DAOQMB010000239         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14010_length_3515_cov_16.536705, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000239 DAOQMB010000000
VERSION     DAOQMB010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14010_length_3515_cov_16.536705"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1304
                     /locus_tag="PLY05_08110"
     CDS             <1..>1304
                     /locus_tag="PLY05_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002048094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNC03305.1"
                     /translation="TELKNQADGEAIHVFGQNLKNLLMAAPAGQRATMGLDPGLRTGV
                     KVAIVDKTGKLVAYTAIFPHQPKNEWHESLAVLAKLCAQHHVELIAIGNGTASRETDQ
                     LAAELIKKHSELKLTKIVVSEAGASVYSASALAAEEFPELDVSYRGAVSIARRLQDPL
                     AELVKIEPKAIGVGQYQHDVNQPQLARGLDAVVEDCVNAVGVDVNTASVALLTRIAGL
                     NKTIAQNLVNFRNEHGAFANRENLKKVPRLGAKTFEQAAGFLRISGGDNPLDSSSVHP
                     EAYPVVEKILRNKGLTMSDLMGNSEKVRSIKAQDYIDERFGLPTIQDILQELEKPARD
                     PRPAFVAPQFMEGVTDIKDLVVGMVLEGVVTNVTNFGAFVDIGVHQDGLVHISMLSES
                     FVKDPHAVVKAGDIVKVKVIEVDVARKRIALTRRLNDKAAAQT"
     assembly_gap    1305..1404
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1405..2253)
                     /gene="hslO"
                     /locus_tag="PLY05_08115"
     CDS             complement(<1405..2253)
                     /gene="hslO"
                     /locus_tag="PLY05_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005400917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNC03306.1"
                     /translation="MIMPDIRQRFYFADSPVRGEIVQLEQSLAAIFSRHQYPAAVQRL
                     LAELVTASVLLTATLKLEGRLSLQAQGKGALKMMMAECNHRHEVRAIAQVAEDADFAD
                     DATLKDLLTDGLLVITLEPEHGQRYQGIVALEGNSIAEYLENYFAQSEQLPTRLWLTA
                     NGVKGSGLLLQALPHTTDEDADRWPRLLQLTETVKNEELLDLSAEEVLYRLYHEESVT
                     LPASEAVSFACTCSREGTAQALMSIGQAECEDILAEQNDIRLHCQFCHNEYVFDASDI
                     KMLFKVQ"
     gene            2665..>3515
                     /locus_tag="PLY05_08120"
     CDS             2665..>3515
                     /locus_tag="PLY05_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HNC03307.1"
                     /translation="MKLNRIALAVLLAAPMAMSAHAGLYSVTPLVGYHSPDQGDAGAY
                     VGLAAGVKLVSSVAAELEYGSASDATLLNANLLISPQSWSNGTLSPYILAGVGQQDLD
                     AIKGVTKERTDTVMNLGGGAFYSLNDVLSLRGEARVIHNSDWSKTDYLVLTGLQYSFG
                     SQPASEVVTAPVYVAPVEPINVVPADTDGDGVVDANDRCPNTPSGWEVGVDGCPLDSD
                     KDRVPNSIDECPNTPNGMQVGANGCPLDEDKDGVTDAKDKCPGTALNVIVDANGCAKT
                     VTKEIKEA"
BASE COUNT          995 a          676 c          754 g          990 t
ORIGIN      
        1 acagaattaa aaaatcaagc cgatggcgaa gcgattcatg tctttggcca aaatttgaaa
       61 aacttgttaa tggctgcacc tgcgggtcaa cgggcaacca tgggtttaga ccccggttta
      121 cggacaggcg taaaagtcgc cattgttgat aaaacaggca aattagtcgc ttatactgct
      181 attttccccc atcaacccaa aaacgaatgg catgaatctt tagcggtttt agccaaatta
      241 tgcgcccaac atcacgtgga gttaattgcc attggtaatg gtactgcatc acgagagact
      301 gaccaattag ccgccgaact gattaaaaaa cacagcgaat taaaattaac caaaattgtc
      361 gtcagtgaag caggggcatc ggtgtattct gcatcagcat tggctgccga ggaatttcct
      421 gagttagatg tctcgtatcg gggcgcggtg tcgattgccc gtcgtttgca agacccttta
      481 gccgaattag tcaaaattga acctaaagcg attggcgtgg gtcaatatca gcatgatgtt
      541 aatcagcctc agttggcgcg tggtttggat gcggtggtgg aagactgtgt taatgcggtg
      601 ggtgttgatg tgaatacggc atcagtggcc ttattaaccc gtattgcagg tttaaataaa
      661 accattgccc aaaacttggt gaattttaga aatgaacatg gtgcatttgc caatcgtgaa
      721 aatctgaaaa aagtgccacg cttaggcgca aaaacctttg agcaagcggc aggctttttg
      781 cgaatttcag ggggagataa tcccttagat agttctagtg tccatcctga agcctatcct
      841 gtggtagaaa agattttgcg taacaagggc ttaacaatga gcgatttaat gggcaatagc
      901 gaaaaagtcc gttccattaa agcccaagac tatattgatg agcgttttgg tttacccacg
      961 attcaagata ttttacagga attagaaaaa ccagcacgtg acccacgacc tgcttttgtt
     1021 gcaccacagt ttatggaagg tgtcaccgac attaaagact tggtggtcgg tatggtgtta
     1081 gaaggtgtgg tcactaatgt cacgaatttt ggtgcgtttg tcgatattgg cgtacatcaa
     1141 gatggcttag tacatatttc aatgttgtca gaaagttttg tcaaagaccc tcatgcggtg
     1201 gtcaaagcag gcgacattgt caaagtgaaa gtgattgaag tggatgtggc acgtaaacgg
     1261 attgcgttaa cacgccgctt aaacgacaaa gctgcggcac aaacnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnttgcac tttaaataac atttttatat cactggcatc
     1441 aaacacatat tcgttgtgac aaaattgaca atgcaaacga atgtcgtttt gttcggccaa
     1501 aatatcctca cattccgctt gaccaatact cattaacgct tgtgccgttc cctcgcgtga
     1561 acaagtacag gcaaagctca cagcttcact ggcaggcaaa gttaccgatt cttcatggta
     1621 caaacgatat aacacttctt cggcagataa atccaacaac tcttcatttt taaccgtctc
     1681 cgttaactgc aataaacgcg gccaacgatc agcatcttca tcagtagtgt ggggcagagc
     1741 ttgcaataat aatccgctac ctttaacacc attggcggtt aaccataaac gagtgggcaa
     1801 ttgttcagat tgagcaaaat aattctctaa atactcagca atactattgc cttccaaagc
     1861 aacaataccc tgataacgtt ggccatgttc aggctctagc gtaatcacca acaaaccatc
     1921 cgttaataaa tctttcaagg ttgcatcatc agcaaaatca gcgtcttctg ctacttgagc
     1981 aattgcccgc acctcatgac gatgattaca ttcggccatc atcattttta atgcaccttt
     2041 gccttgtgct tgtaagctta aacgaccttc aagttttaag gtggctgtta atagcacact
     2101 cgctgtgact aactcggcta ataaccgttg aacagccgca ggatactgat gacgactaaa
     2161 aatcgcggct aaactctgtt ctaattgcac aatttcacca cgaacagggc tatcagcaaa
     2221 ataaaaacgt tgacgaatat cgggcataat catagtcctg ttcaatagtt acaaaagcag
     2281 attgtatata gggcgttttt ggcaagtctc aatagacttt catcggtatt ttagagcggt
     2341 ttaacagttt ttattagttt aaagacacat ttatcactat tatccgcagc aattgtgtca
     2401 aaagaagcgg ctttgtcata catcttgcta taaaaatatc catttattgg gtacaatagt
     2461 ttttaagatg atacaaaaaa cgttgttttg tttgacatca agacttgacc aaataggcgt
     2521 aactcgttac tttttgcaag actgttggca ttatagatat gttgtgcttg acaaaaagag
     2581 ctttgatgct cttttgactt gctaagtaaa ggtttgaata acgccacctt aggtaaaaga
     2641 cattcctaaa tcaggaggaa ctcaatgaaa ctcaatcgga ttgcactcgc tgtattatta
     2701 gcagccccga tggctatgtc tgctcatgca ggtttatatt ctgtaacacc attagtgggt
     2761 tatcactcac ctgatcaagg cgatgctgga gcttatgtag gtttggcagc tggtgttaaa
     2821 ttagtctcca gtgttgcggc agaattagag tatggctctg cttctgatgc aacattactc
     2881 aatgctaatc ttttaatttc gcctcaatct tggagtaatg gtactttatc tccttacatt
     2941 ttagcaggtg ttggacaaca agatttagat gcaattaaag gtgtgactaa agaaagaact
     3001 gacactgtga tgaatttagg tggcggtgct ttttattctt taaatgatgt attgtctctt
     3061 cgtggtgagg ctcgtgtcat tcataactcc gattggagta aaacagacta tttggtttta
     3121 acgggtttgc aatactcgtt tggtagtcaa ccagctagtg aagttgtcac tgctcctgtt
     3181 tatgttgcac ctgttgagcc aatcaacgtt gtgcctgctg ataccgatgg cgatggtgtg
     3241 gttgacgcaa atgaccgttg tccaaatacg ccctctggtt gggaagttgg agtggatggt
     3301 tgtcctttag atagcgacaa ggatcgtgta cctaatagta ttgacgaatg tccaaataca
     3361 ccaaacggta tgcaagttgg tgctaatggt tgtccattag atgaagataa agatggtgtg
     3421 actgacgcta aagataagtg tccgggtaca gctttaaatg tgattgttga tgccaatggt
     3481 tgtgctaaaa cagtgactaa agaaattaaa gaagc
//
LOCUS       DAOQMB010000240         3505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14088_length_3505_cov_14.191014, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000240 DAOQMB010000000
VERSION     DAOQMB010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14088_length_3505_cov_14.191014"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            471..1865
                     /locus_tag="PLY05_08125"
     CDS             471..1865
                     /locus_tag="PLY05_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HNC03308.1"
                     /translation="MLTEFRIKNFKAWQDTGDVRLAPLTVIFGANSAGKSSLGHLLLA
                     LKQTVLSVDRKRALQLGDENSLIDLGTFAECLHNHDIEQQLKFNLGWKLPTKLEVKDP
                     ITQTRFVGNELSLSVAMKSDVREQPIVQKLNYSLKEEQKPVLQIAYTSDGKNKLELTS
                     EEYKFVRTTGRVWKLDAPDKFYRISDQSRARFQNADFLSDFALQTEAALNSVYYLGPL
                     RDHPKRIYSWSGETPDSVGQRGEFAIAAILAANAQERKLSRGFRMRQYRFDEFIAQWL
                     KDLGIIESFVVKPVAEGRKEFEVLVKTHASASEVKITDVGFGVSQVLPALVQAFYCPF
                     DSTIWMEQPEIHLHPQVQAELADVFISATQARENGKERNVQLIVESHSEHFLNRLQRR
                     VAEGVVKPEDVAVYFCRRAGATTELEPLRLNMFGEIENWPENFFGDEMADIAGRTLAA
                     VQRRKELKAKGQDK"
     gene            1862..2383
                     /locus_tag="PLY05_08130"
     CDS             1862..2383
                     /locus_tag="PLY05_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03309.1"
                     /translation="MNKLVIDTNVLLVANEQHHDVSDECIEECIRRLRTIQESGITII
                     DDGYRILGEYQHKTRLNPPKGVGDVFLKWLLRNPQRIESVSLNETAQDCFVEFPDSVL
                     EPLFDAPDRKFAAVAYAHPDKPPILQAADCKWLDWWPVLNTKGVRVEFLCPTDACKFY
                     QNKFPNKAKPELP"
     gene            2383..3081
                     /locus_tag="PLY05_08135"
     CDS             2383..3081
                     /locus_tag="PLY05_08135"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HNC03310.1"
                     /translation="MSDFFRFPHTPHLAWLAVGTPRDDKVLSSQEVAALLAGDVVVEE
                     KLDGANLGISLDTKGQLRSQNRGQYLSEPHAGQFSRLPAWLAQHREALYSVLTHDLIL
                     FGEWCAARHSLDYAALPDWFLLFDVYDRSVGRFWSTSRRNILAHNTGLLTVPQVMHGK
                     FTLAELKTVVMDTPSHYRQGALEGVVIRSESADWCESRAKLVRPDFTQSIETHWRKRT
                     IEWNRVDYSANISS"
BASE COUNT         1027 a          602 c          838 g         1038 t
ORIGIN      
        1 catgttttta gcgcgaaaaa cacgataaat gaattgctta tttttttaga aaaatatgtt
       61 tagatatgag gtaagcgttt tatatggcgc agttttgatg tgaaagtggc gcagttatgg
      121 cgcagtttta aatataatgt cattcagact ggcatgtttt agataagttg cacgtgctat
      181 taaatgtcaa gattaaatta atcaaatgag tatcttctaa tgagcagtat catgaagcga
      241 gctaaatcaa tttaaatcag tcattggagc ctgtataaat taaactgtgt caatgaagca
      301 gagcagtagc atttccaata tatagtaaca atatcaccaa aagcctcata agtttttgag
      361 aaaagtgttt gtttacggct tgatagtcgt caagaggatg ggctgttgtt tactaagcgt
      421 ataggtgtgg aagttaagct actagctatt tagataatga gaaattaatc atgttgaccg
      481 aatttagaat aaagaatttt aaagcatggc aagatactgg tgatgttcgg ttggcaccac
      541 tcacggtaat ttttggtgcc aatagtgctg gaaagagcag cttgggacat ttattgttag
      601 cactcaagca aactgtattg tcggtagatc gcaaacgggc attacagttg ggtgatgaaa
      661 attcgctgat tgatttggga acttttgctg aatgcttaca caatcatgat attgagcagc
      721 agttaaaatt taatttgggt tggaagttgc ccacaaaatt ggaagtaaaa gatccaatca
      781 cacaaactcg gtttgttgga aacgagttga gcctttcagt agccatgaaa agtgatgtaa
      841 gagaacagcc tattgttcag aagttgaact attccttgaa agaagaacaa aagccagttc
      901 ttcaaatcgc ttatacgagt gacggcaaaa acaagctgga gcttacttcg gaagagtata
      961 aatttgtcag aacaacaggt agggtgtgga agcttgatgc accagacaaa ttttatcgta
     1021 tttcagatca aagccgagcg cgcttccaaa atgccgattt tttgtcagat tttgcgttgc
     1081 aaactgaggc tgccctcaac agtgtttatt atttaggccc tttacgcgat catcccaaac
     1141 gtatttattc ttggtcaggt gaaacaccag atagtgtagg acaacgaggt gagtttgcga
     1201 ttgcagcaat tttggctgcc aatgctcagg agcgtaagct cagtcgtggt tttagaatga
     1261 gacaataccg ctttgatgag tttatagcac aatggctaaa agatttaggc attatcgaaa
     1321 gctttgttgt caagcctgtg gccgaaggtc ggaaagagtt tgaggttttg gtaaaaaccc
     1381 atgccagtgc cagtgaagtg aagattaccg atgtgggttt tggtgtttct caagttttgc
     1441 cagcattggt tcaggccttt tattgtcctt ttgattcgac tatatggatg gagcaacccg
     1501 agattcattt gcatccacaa gtacaggctg aattggcgga cgtatttatc tctgcaacac
     1561 aggcgcgtga aaatggtaaa gagcgtaatg ttcagttgat tgtagaaagt cattctgaac
     1621 actttcttaa tcgcttgcaa cgtcgtgtag cggaaggtgt ggtcaagcca gaagatgttg
     1681 ctgtgtactt ttgtaggcgt gcgggtgcaa cgacagagct tgagccactg cggttgaaca
     1741 tgtttggtga aattgaaaat tggccagaaa acttttttgg tgacgaaatg gcagacatag
     1801 ctggtcgcac attggcagca gtacagcgta gaaaagaatt aaaagccaaa ggacaggata
     1861 aatgaacaag ctagtcattg ataccaatgt gttgctagtg gcaaatgagc aacatcatga
     1921 tgtatctgat gagtgtattg aggagtgcat tcgtcgattg cggactatac aagaatctgg
     1981 tatcacaata attgatgatg gttaccgaat tctcggtgaa tatcaacata agacacgact
     2041 caatccaccc aagggcgtag gtgatgtttt tttgaagtgg ttgttgcgta acccacaaag
     2101 gatagagtcg gttagcttga atgagaccgc acaagattgt tttgtagagt ttcctgattc
     2161 tgtattggaa cctttgtttg atgcaccaga tcgtaaattt gccgccgtcg catacgctca
     2221 tccagacaaa cctcctattc tgcaagcagc tgattgcaaa tggctggatt ggtggccagt
     2281 tttaaatacc aaaggggtgc gagtcgagtt tttgtgtcca acggacgctt gtaagtttta
     2341 ccaaaacaag ttccctaata aggcaaagcc tgagttacca taatgtctga cttcttccgt
     2401 tttccacaca caccacactt agcttggttg gcagtaggta caccgcgcga tgacaaagtc
     2461 ctgtcttctc aggaagtagc agcattactt gctggggatg tcgtcgtgga agaaaaatta
     2521 gatggagcca acctagggat ttcattagat acaaaaggac aattgcggag tcaaaaccga
     2581 gggcaatacc tgtccgaacc ccatgcaggt caattttccc gtttgcctgc atggttagcc
     2641 cagcataggg aggccttgta ttccgtactc acccacgatt tgattttgtt tggcgagtgg
     2701 tgtgctgcac gccattcgct cgactatgcc gcactaccag attggttctt actgtttgat
     2761 gtttatgacc gttctgttgg ccgcttttgg agtacatcaa gacgcaacat actagcgcat
     2821 aacacaggat tattgacagt accacaagta atgcacggta agttcacatt ggctgaattg
     2881 aagacagtag tgatggatac gccaagccac tatcgtcagg gggctttgga gggggtggtg
     2941 attcgttctg agtctgctga ttggtgtgag agccgtgcta agttggttcg ccctgacttt
     3001 acacaatcga tcgaaaccca ctggcgcaaa cgcacgattg agtggaatag agttgactat
     3061 tcagcaaata tttcgtcatg aatccactgc aactggccat tatagaaaaa ataggaaact
     3121 ataatgactt taaacatgta attgtttttt aaacacttta tttggttgga gcttgtcatt
     3181 tcttgggtaa ttaggtctat ggctattgct gtcgtgtaga agagttcaag ctgatttaaa
     3241 aaaattagta tgactatgat tcttccaata catctaaaca atcatcaata tttgtcatgt
     3301 ttttggtgtg aaaaaaatga caaatgaatt gcttattttt taagaaaaat atgcttagtg
     3361 atgtctgagc gttttatatg gcgcagtttt gatgatggag atatgagaaa tggtgaagtg
     3421 ctaataaaag ttacgtatga agtcatcgat tcatgtaatg tctaataaag ttgtgtagtg
     3481 atggtaatgt caggggactt gatat
//
LOCUS       DAOQMB010000241         3495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14171_length_3495_cov_16.136628, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000241 DAOQMB010000000
VERSION     DAOQMB010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14171_length_3495_cov_16.136628"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /gene="leuC"
                     /locus_tag="PLY05_08140"
     CDS             complement(<1..1370)
                     /gene="leuC"
                     /locus_tag="PLY05_08140"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNC03311.1"
                     /translation="MSAKTLYDKLWDDHLVKQRDDGSCLIYIDRQLLHEVTSPQAFDG
                     LKLANRQPWRLDANIATPDHNVPTDKAERSQGIAGIKDETSRIQVQTLDDNCKEFGVV
                     EFDIFDERQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFACLAHGIGTSEVEHVLA
                     TQCLVQKKSKNMLVKVDGVLGEGVTAKDVVLAIIGKIGTAGGTGYAIEFGGQVFRDMS
                     MEGRMTVCNMAIEAGARVGMVAVDEKTIAYFKDKPFAPKAEQWDAAVAYWQGLTSDAD
                     AQFDAVVELDGNAIEPQVSWGTSPEMVLPISAKLPTLDSANDAVQRSSWQRAYQYMGL
                     TPEQAITDIKLDRVFIGSCTNSRIEDLRSAAAVIKGRKVANTVKQAMVVAGSGLVKKQ
                     AEAEGLDKIFTEAGFEWREPGCSMCLAMNADKLLSGEHCASTSNRNFEGRQGNGGRTH
                     LVSPAMA"
     gene            complement(1504..1725)
                     /locus_tag="PLY05_08145"
     CDS             complement(1504..1725)
                     /locus_tag="PLY05_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011281225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HNC03312.1"
                     /translation="MINQVVAGRYRHYKGKEYEVLAVATHSETEEKLVVYRLLYGDFS
                     YWVRPLTMFTETVIVDGIEQPRFSLIEKS"
     gene            complement(1722..2021)
                     /locus_tag="PLY05_08150"
     CDS             complement(1722..2021)
                     /locus_tag="PLY05_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03313.1"
                     /translation="MVKYVIGERQVLAKMKNGEFRLVKLQVGLPEPLENRWQSEIALD
                     GLYPNVVKVPGVDSFQCLNLAFGVVRTALEKFIESGGQLYWKDGSGPLGLVDIFS"
     gene            complement(2033..2404)
                     /locus_tag="PLY05_08155"
     CDS             complement(2033..2404)
                     /locus_tag="PLY05_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC03314.1"
                     /translation="MRFCLIIVILFVNTCVLAQDLYRWQDEQGKWHFGDAASTNGQNR
                     QIIEPAEYTTNVVKTKKLRLPSSKPTKVKIVKRVKSKKTESLEEKKLRCEAKREELRF
                     QAFRYEERNQYDRECVSDMKW"
     gene            2561..>3495
                     /locus_tag="PLY05_08160"
     CDS             2561..>3495
                     /locus_tag="PLY05_08160"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC03315.1"
                     /translation="MASLPSVLTGSINSLSLAANTFSLCTPLFGLALLKTLPIKPLQK
                     ACTQGSTFIAETWIGINSKMFDLTHQTQYNITGDLDKISYNGWYMVVANHQSWADIFI
                     LQKIFNRHAPFFKFFLKQELIYVPVMGLCWSALDYPFMKRYSKEFLAKHPELKGKDLE
                     TTRKACEKFKHTPVSIMNFLEGTRFTPQKHQSQQSPYQHLLKPKAAGIAYALNIMGER
                     IDTLIDVTIHYPDDRPSFWEFMCGTSKNIDVHIQTRPIPAEFNRGDYENDEVFRKNFQ
                     TWINQLWQDKDKLLGEMAKRYSQPLQNIIEFKKAS"
BASE COUNT         1075 a          796 c          583 g         1041 t
ORIGIN      
        1 gccatcgctg ggctgactaa atgcgtacga ccaccattgc cttgacgacc ttcaaagtta
       61 cggtttgagg ttgacgcaca atgctcacca gacaataatt tgtcagcatt catcgccaaa
      121 cacattgaac aacctggttc acgccattca aaacctgctt cggtaaagat tttgtctaaa
      181 ccttcggcct cggcttgttt ttttaccaaa ccagaacctg caaccaccat tgcttgtttg
      241 acggtattgg ccactttacg gcctttaatc actgctgccg cggagcgcaa atcttcaata
      301 cgtgagttgg tacaagaacc aataaacaca cgatctaatt taatatcggt aatcgcttgt
      361 tcaggtgtta agcccatata ttgataagca cgttgccaag agctacgttg taccgcatcg
      421 ttagcactgt ctaaggtcgg taatttagca ctaattggca ataccatttc gggtgaagtt
      481 ccccaagaca cttgcggctc aatggcatta ccatctaatt caaccaccgc atcaaattga
      541 gcatcagcat cagatgttaa gccctgccaa tacgcgacgg ctgcatccca ttgctccgct
      601 ttaggtgcaa atggcttgtc tttgaagtaa gcaatggttt tttcgtccac cgccaccatg
      661 cccacgcgcg cacctgcttc gatagccata ttacacacgg tcatgcggcc ttccatgctc
      721 atgtctctga acacttgacc accaaactca atcgcgtagc ctgtaccacc tgccgtacca
      781 attttgccaa taatcgctaa aacgacatct ttagcagtca caccttcgcc caatacgccg
      841 tcaactttga ccaacatatt tttggatttc ttctgcacta aacattgagt cgctaatacg
      901 tgctctactt cgctagtgcc aataccatgt gctaaacaag caaatgcacc atgcgttgcc
      961 gtatgagaat caccacaaac tacggtcata ccaggtaaag ttaaaccttg ctctgggcca
     1021 accacatgaa caatgccttg acgctcatca aaaatatcaa actcaacgac accaaactct
     1081 ttgcagttgt catctaaggt ttgtacttga atgcgtgaag tttcgtcttt aatgccagca
     1141 ataccttgac tacgctcggc tttgtcggtt ggcacgttgt ggtctggcgt ggcaatgttg
     1201 gcatctaaac gccaaggttg acgattggcc aactttaagc catcaaaagc ttgagggctg
     1261 gtaacttcgt gtaataattg acggtcaata taaattaaac aagaaccatc atcacgttgc
     1321 ttgactaagt ggtcatccca caatttgtca tataaggttt tggctgacat ggctatttcc
     1381 tcatgtgtca ttataggact tacgcgtaag tcctgactat ttaaatacat gagtcgattg
     1441 taagagcgcg tctaaaataa aacaatttta taatttttat aaattaaata actttttgga
     1501 atactaagac ttttcaatca aactaaaacg tggctgctcg atgccatcca caataacggt
     1561 ttctgtaaac atcgttagtg gccgcaccca ataagaaaaa tccccatata aaagacgata
     1621 taccactaat ttttcttccg tttcactgtg agtagcaacg gcaagcactt cgtattcttt
     1681 acctttataa tgacgataac gacctgcaac aacttgattt atcatgaaaa aatatccact
     1741 aatccgagag ggccagaacc atctttccaa tataattgac cacctgattc aataaacttt
     1801 tctaaggccg tacgtaccac gccaaaggct aaatttaaac attgaaacga atcaacacca
     1861 ggcactttaa ccacattggg atacaagcca tctaaagcta tttccgattg ccaacgattt
     1921 tctaaaggct caggtaaacc cacttgtagc ttcaccaatc gaaactcacc atttttcatt
     1981 ttggccaaaa cctgacgttc gccaatgaca tatttcacca tttgtttatc atttaccatt
     2041 tcatgtcact cacacattcg cggtcatact gattacgttc ctcataacga aatgcttgaa
     2101 accgcaactc ctcgcgcttt gcttcgcaac gtaatttttt ttcttctaaa ctttcagttt
     2161 ttttagactt aactcgttta acaatcttaa cttttgttgg tttgcttgag ggtaatctta
     2221 acttttttgt tttcacaaca ttcgttgtat attcagcagg ctcaataatt tgacgatttt
     2281 gaccattcgt actagccgca tcgccaaaat gccattttcc ttgttcatct tgccagcgat
     2341 ataagtcctg agctaaaaca caagtattaa caaataatat cactattatt aaacaaaaac
     2401 gcatagtata tccttataaa aaaatcctaa gcatttgatt gtagttttaa attagtaaaa
     2461 aaaccatcca aaattactgt ctaaaaaata actatccaaa gccaatcaaa taatatacac
     2521 ttgtatatta tctaataaat caatgtgtat tgaggttatt atggcatctc taccttcagt
     2581 cttaacgggt agcattaata gtttatccct cgctgcaaat acctttagtc tttgtactcc
     2641 cctatttggt ttagcattgc taaaaacttt acccatcaaa ccattacaaa aagcctgcac
     2701 acaaggcagt acctttattg cggaaacatg gattggtatc aatagtaaga tgttcgattt
     2761 aacccatcaa acgcaataca acattacggg tgatttagat aaaatcagtt ataacggctg
     2821 gtatatggtg gttgccaacc atcaatcttg ggcagatatt tttattttgc aaaaaatctt
     2881 taatcgtcat gcgccatttt ttaaattttt tctcaagcaa gaattaattt atgtgcctgt
     2941 catgggctta tgttggtctg cgctagacta cccttttatg aaaagatata gcaaagagtt
     3001 tttagctaaa catcctgagt taaaaggcaa agatttagaa acaacccgta aagcctgcga
     3061 aaaatttaaa catacgcctg tttcaattat gaactttctg gaaggcacac gttttacacc
     3121 acaaaaacat caaagccaac aatcccctta tcaacactta ctgaaaccca aagcagcggg
     3181 tattgcttat gccctaaata tcatgggaga gcgtattgac acattgattg atgtgacaat
     3241 tcattatcct gatgaccgac ctagcttttg ggaatttatg tgtggcacgt ctaaaaatat
     3301 tgatgtgcat atacaaacac gccctattcc tgcggaattt aatcgtggtg attatgaaaa
     3361 tgatgaagtg tttagaaaaa actttcaaac ttggattaat caactgtggc aagataaaga
     3421 caagctgcta ggtgaaatgg ccaaacgtta cagtcaaccc ttacaaaata ttattgaatt
     3481 taaaaaagcc agtta
//
LOCUS       DAOQMB010000242         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14205_length_3490_cov_12.384862, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000242 DAOQMB010000000
VERSION     DAOQMB010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14205_length_3490_cov_12.384862"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..718
                     /locus_tag="PLY05_08165"
     CDS             35..718
                     /locus_tag="PLY05_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNC03316.1"
                     /translation="MKTICYVDGYNLFYGCLKHSQDKWLDLYKLLYQQIIYPQNPKSQ
                     LVKIKFFTADIKAKIATHGQDANEAQQAYHRALEKCYPDTIEIIKGYYSLEKAELLAY
                     QNPPDKQNRVEVWRLEEKQTDVNIAVHAYRDAIKGDAEQLVFVSNDTDLEPALSMIRQ
                     DMGDTVKMGVIFPIRQQEGLERRPPNGRLSKYTDWTRRYITAEELAASHLPLKIPTNK
                     KTIFKPTYW"
     gene            739..>2183
                     /locus_tag="PLY05_08170"
     CDS             739..>2183
                     /locus_tag="PLY05_08170"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNC03317.1"
                     /translation="MNNLPKFQEEYSAKIPALTLLVSLGWNFLSPAQALASREHKTSN
                     VVLKNILREELKKRHFHYAGKDYPLSEKAIDTLLSEVCNPALNEGLGVANERIYNHML
                     YGITVTEFVDGKKASVTIPLIDWANFANNRFDFTEEFSVLRENGLDSRRPDIVCFVNG
                     LPLVVIEAKRPDGHAGKAPTIAEGISQSLRNQRHDEIPSLFAYTQLLLSINGIEGRYG
                     TTLTKEKFWAAWREEDISDAAMYAIKNTKLNINQLNTLFSYRPVGDRKWYEQLVAGGE
                     LMVTEQDRLLISLLQPSRLLEMVRYFVLFDKKVGKIIARYQQVFGIKRLIERINQQRP
                     DGGREGGVIWHTTGSGKSFTMVFLSKALILHDSLKKCRIIVVTDRIDLESQLSKTFAS
                     GGELASKKDREEALATSGRGLAEKIGKGQERIVFSLVQKFNTAAKLPECYNDSPDIIV
                     LIDEGHRSQGGENHIRMKQALPKAAFVAFTGT"
     assembly_gap    2184..2283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2284..>3490
                     /locus_tag="PLY05_08175"
     CDS             <2284..>3490
                     /locus_tag="PLY05_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC03318.1"
                     /translation="KGQIACDSKISAIKYKQFLDEVMAILEPEQQFETAVVISAPDTR
                     EGNTDVDEKSMPLVSKWWKDNVGSQDESVYTKQVISRFEDDPKLKLLIVVDKLLTGFD
                     EPQNAVLYIDKPLKSHNLIQAIARVNRLHDKKKFGLLIDYRGILAELDTTIANYQDLA
                     NRTQGGFEIDDLLGLYSQMSSEYKRLPRLYQNLWAIFKDVKNKNDIEQLRQVLIPHVQ
                     EVNGELVDVHLKVRDDFYEALTEFASCLQIALQSMSFFDDKSFSDADRQHYKDTVKQL
                     SSLRQLVRRDAGETVDYDQYAEQVKKLLDKHVVGVGVKESEGVYEVGKMGRQLKPEEW
                     NADKTRNETDIIKTRVTKMIEQELRDDPYAQESFSKLLRQAIEEAESLFDHPLKQYLL
                     FSEFEEKVK"
BASE COUNT         1059 a          635 c          756 g          940 t
ORIGIN      
        1 ttaggcggtt ttttcatttt agagtttaaa aatagtgaaa acaatttgtt atgttgatgg
       61 ttataacttg ttttatggct gtttaaaaca cagtcaagat aaatggctag acctttataa
      121 attgctgtat cagcaaatta tttatccgca aaatccaaaa agtcagcttg ttaaaatcaa
      181 attttttacg gctgacatca aagctaaaat tgctacgcat ggtcaagacg caaatgaagc
      241 gcaacaagcc tatcatcgtg ccttagaaaa atgttatccc gatactatag aaattattaa
      301 gggttattac tctttagaga aagctgagtt gttagcctat caaaaccctc ctgataaaca
      361 aaaccgcgtt gaagtatggc gtttagaaga aaagcaaaca gacgttaata ttgccgttca
      421 tgcttataga gatgctatca agggcgatgc agagcaatta gtgtttgtgt ctaatgatac
      481 cgatttagag cctgctctta gtatgattcg ccaagatatg ggcgatacag taaaaatggg
      541 tgttattttt cctattcgcc agcaagaggg cttggagcga agacctccta atgggcgttt
      601 aagtaaatat acggattgga ctcgacgtta tattaccgcc gaagaattag ctgcatcaca
      661 cttgccatta aaaataccca ccaataaaaa aactattttc aaacccacat attggtaaca
      721 gtgttgcaag gaatagccat gaataatctc cccaaatttc aagaagaata cagtgccaaa
      781 attcctgcat taactttgtt ggtatcactg ggctggaact tcttatcgcc agcgcaagca
      841 ctcgcctcaa gagagcataa aaccagtaat gtagtcctaa aaaatatttt acgcgaagaa
      901 ttaaaaaaac gtcattttca ctatgcaggt aaagactatc ccctcagtga aaaagctatt
      961 gataccttgt tgtcagaagt gtgcaatcca gccctaaatg aaggtttagg cgtggctaat
     1021 gagcgtattt ataatcatat gctgtacggt attaccgtta ccgagtttgt agatggtaaa
     1081 aaagccagtg tcacgattcc tttaatagat tgggctaact tcgctaataa tcgttttgat
     1141 tttaccgaag aatttagtgt tttacgcgag aacggactgg attcgcgccg tcctgatatt
     1201 gtttgttttg tgaacggctt acctttagtg gtaatagaag ccaagcggcc agatggtcat
     1261 gcaggtaaag ctcccaccat tgccgaaggt atttcgcagt cattgcgtaa tcagcgtcat
     1321 gatgagattc cttccttgtt tgcttatacg caactgttgt tatctattaa cggcatagaa
     1381 gggcgttatg gcacaacctt aaccaaggaa aaattttggg cagcatggcg cgaagaagac
     1441 attagcgatg ctgctatgta tgccattaaa aataccaagc tcaatatcaa tcaacttaat
     1501 acgctatttt cttatcgccc cgtaggcgat agaaagtggt atgagcagct tgtggctggt
     1561 ggtgagttaa tggtgacgga gcaagaccgc ctactcatta gcttactgca accgtctcgc
     1621 ttattagaga tggtgcgtta ttttgtgttg ttcgataaaa aagtcggcaa gattattgcg
     1681 cgttatcagc aagtattcgg cattaagcgg ctgattgagc gtatcaatca gcaacgaccc
     1741 gatggtggac gagaaggcgg tgtgatttgg cacacgacag ggtcaggtaa atcgttcacg
     1801 atggtatttt tatcgaaagc cttaattttg catgacagtc tcaaaaagtg ccgcattatt
     1861 gtcgtcactg accgtatcga cctagagagc caattaagta aaacctttgc ctctggtgga
     1921 gagttagcca gtaaaaaaga ccgtgaagaa gcactcgcta catcgggcag aggcttggcc
     1981 gaaaaaattg gcaaaggtca agagcgtatt gttttctctt tagttcaaaa gtttaatacc
     2041 gccgctaaat tgcccgaatg ctataacgac agccccgata ttattgtgct aattgatgaa
     2101 ggccatcgta gtcaaggtgg ggaaaatcat atccgtatga aacaagcctt accgaaagcg
     2161 gcttttgttg cgtttacggg tacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnntaaaagg ccaaattgcc tgtgatagca agatttcggc tatcaagtac aaacagtttt
     2341 tagatgaagt gatggcaatt ttagagccag aacaacagtt tgaaacggca gttgtgattt
     2401 ccgcacccga tacgcgtgaa ggcaataccg atgttgatga aaagtctatg ccgctagtga
     2461 gtaaatggtg gaaagataat gtcggctcac aagatgagtc cgtttatacc aaacaggtaa
     2521 tcagccgttt tgaagatgac ccaaaactca agttgttgat cgttgtcgat aaactgttaa
     2581 cgggttttga tgagccacaa aatgccgtgt tatatattga taaaccgtta aagtcgcaca
     2641 accttatcca agccattgcc cgtgtcaatc gtctgcacga taaaaagaag tttggcttat
     2701 taatagatta tcgtggcatt ttagctgaat tagacaccac aattgccaac tatcaagact
     2761 tagccaaccg tacacagggc ggttttgaga tagatgattt acttggctta tatagccaaa
     2821 tgagtagtga atataaacgt ctgccgcgtc tttatcagaa cctttgggcg atatttaaag
     2881 atgtcaaaaa caagaatgac atcgagcagt tgcgacaagt attgattcct cacgtccaag
     2941 aagtgaatgg cgagttggtt gatgttcatt tgaaagtcag agatgacttt tacgaagcgt
     3001 taacggagtt tgcgagttgc ctacaaattg ccttgcagtc aatgagcttt tttgatgata
     3061 agagctttag cgatgccgac cgtcagcact ataaagacac ggtaaaacaa ctcagcagct
     3121 tacgacaatt agtcagacgt gatgcagggg aaacagtgga ctatgaccaa tatgctgaac
     3181 aagtcaaaaa gctattggat aagcacgttg ttggtgttgg ggtaaaagag tcagaaggcg
     3241 tttatgaagt cggtaaaatg ggtcgtcaac tcaaaccaga agaatggaac gccgacaaaa
     3301 cgcgcaatga gactgacatc attaaaacac gcgtcactaa aatgattgag caggagttac
     3361 gcgatgaccc ctatgctcag gagtcttttt ctaagttatt gcgtcaagcg atagaagaag
     3421 cagaaagcct atttgaccat ccacttaaac agtatttgtt gttttctgag tttgaagaaa
     3481 aggtaaaaga
//
LOCUS       DAOQMB010000243         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14235_length_3485_cov_11.604082, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000243 DAOQMB010000000
VERSION     DAOQMB010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_3485_cov_11.604082"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..886
                     /locus_tag="PLY05_08180"
     CDS             272..886
                     /locus_tag="PLY05_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03319.1"
                     /translation="MSSNFSWFLRFVIYIPLTVAILFMVSYSYLTSRFDKNFSECLQK
                     VPLNQQNVSAREMTSFVGCLKSKGNFFISRMMREERLYQYTQPKMQCELVGKWYVSDG
                     YQEYWLTIQSNSQFFTEPLLNAKNQESGIILHKGIWISVDKQTAIQFFDGEYFWPIRE
                     YKVNWLSDRHFALVNILEDKQVYFYRNSPLDQACSNSKNITKSN"
     gene            960..2858
                     /gene="htpG"
                     /locus_tag="PLY05_08185"
     CDS             960..2858
                     /gene="htpG"
                     /locus_tag="PLY05_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNC03320.1"
                     /translation="MTDTVQKETLGFQTEVSKLLHLMIHSLYSNPEIFLRELISNASD
                     AADKLRFEALTHADWLSEDPELKIWIDVDKEAKTITLRDNGIGMSRQEVIDNLGTIAR
                     SGTSEFVKNLTGDQKKDAQLIGQFGVGFYSAFVVAQKVEVYTRRAGAAASDAVKWESE
                     GEGAFTVESIDKAERGTTIVLHLRPTQEEFADAFRLKNIIHKYAEHIAIPVCMKAQKW
                     NEEGKEEVGDYEVVNKAAALWTRPRNEISEEEYQGFYKHIAHDYENPLAWNHNKVEGK
                     LEYTSLLFVPKRAPHDLYQRDGVRGLKLYVQRVFIMDDAEQFLPMYLRFVKGIVDSND
                     LPLNVSRELLQSGKVVDSMKSALTRRVLDMLTSMADSKAEDYAVCWAQFGNVLKEGVA
                     EDQSNQDKVLKLLRFASTLSGDAVESVALADYVARMKEGQEKIYYLTAENFTAASQSP
                     HLEVFRKKGVEVLLLTDRLDEWLMTHLTEFDGKQFQHVAKGDLDLGSLSSEEDKKAEE
                     QLQETSQPLVERLQKILADKVQAVKATQRLTDSPACLVRSQYDLGAAMRQLLEASGQK
                     LPESKPTLEINPSHPLVARLSNTNDDARFETLALIVLDQATLAEGSALADPAAYVKRL
                     NSLLMELA"
     gene            2960..>3485
                     /locus_tag="PLY05_08190"
     CDS             2960..>3485
                     /locus_tag="PLY05_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC03321.1"
                     /translation="MSLSKLRVAVLGGGSFGTVIANLAASNGCQTTQWLRDVELVAEM
                     QATHQNTRYLKGFDLHPTLQFSTDLAQTVSQADLVFVAIPSKAFRQVIQTAKPHFQAK
                     QMLVSMTKGIEADTFSLMSQIIREEVPNARLGVLSGPNLAKEIAAKMPAGTVIASLDP
                     DLRATVHQVLACPYF"
BASE COUNT         1073 a          607 c          765 g         1040 t
ORIGIN      
        1 tggatgcaaa gattaactca acattttcaa aaagttgtgt ggttaaatcc tgagtcagaa
       61 agagcgtggc tgcacacaca atcaattagt cacattaaaa agttattaga tgagcgtatg
      121 tatccattaa ccttagaggg tttggaggca gccagtcgat atttgagcaa ataaaatttg
      181 ccagccccta gaggctgttg gcgtttggcg tgtgagctgt aatcaaaacg tcaacagacc
      241 ttaaagataa taaagaaaca aggagaatag agtgagcagt aattttagtt ggtttttgcg
      301 ttttgtaatt tatatcccgt taacggtagc tattcttttt atggtttcat atagttactt
      361 gacttctcgt tttgacaaga actttagtga atgcttacaa aaagttcctt taaatcaaca
      421 aaatgtatcc gctcgagaaa tgaccagttt tgtgggctgt ttaaaaagca aaggtaattt
      481 ttttattagt cggatgatgc gagaagagcg actatatcaa tatacacaac cgaagatgca
      541 gtgtgaatta gtcggtaagt ggtatgtttc cgatggctat caagagtatt ggttaactat
      601 tcaatctaat tctcagtttt ttactgagcc attgctaaac gcaaaaaatc aagagagtgg
      661 tattatattg cataagggca tttggatttc tgtagataaa caaacagcaa tccaattttt
      721 tgatggcgaa tatttttggc caatcagaga atataaagtc aattggttaa gtgatagaca
      781 ttttgcattg gtcaatattt tagaagataa gcaagtttat ttttatcgta attcgcctct
      841 agatcaagca tgtagcaatt caaaaaatat aacaaaatcc aactaaacgc ttgaagttaa
      901 tgtcttcacc cccatgttga ggaacacttg ataatttttt tattaaagga gtcctcaaca
      961 tgactgatac tgtacaaaaa gaaaccttag gctttcagac tgaagtctcc aaactattac
     1021 atctcatgat tcactcgtta tatagtaatc ctgagatttt cttgcgtgaa ctcatttcta
     1081 atgcctctga tgctgctgat aaattacgtt ttgaagcctt aactcatgct gattggttaa
     1141 gtgaagatcc tgagttaaaa atttggattg atgttgataa agaagcaaaa actattacct
     1201 tgcgtgataa cggtattggc atgagccgcc aagaagtaat tgataattta ggtacgattg
     1261 cacgctctgg cacatcagag tttgttaaaa atttaacagg cgatcaaaag aaagatgctc
     1321 aattaattgg tcaatttggt gttggttttt attctgcctt tgttgttgcc caaaaagtag
     1381 aagtctatac ccgtcgtgca ggggcagccg cttctgacgc ggtgaagtgg gaatctgaag
     1441 gcgaaggcgc atttactgta gaaagcattg acaaagctga gcgtggcaca accatcgttt
     1501 tacatttacg tccaacccaa gaagagtttg ctgacgcttt ccgcttaaaa aatatcattc
     1561 acaaatatgc cgaacatatt gccattcctg tgtgtatgaa agcacaaaaa tggaatgaag
     1621 aaggcaaaga agaagtgggt gattatgagg tcgtgaataa agcagccgct ttatggacac
     1681 gcccacgcaa cgaaattagc gaagaagaat atcaaggttt ttataaacac attgcccatg
     1741 attatgaaaa cccattagca tggaaccaca ataaagttga aggtaaatta gaatatacct
     1801 cgttgttgtt tgtgccaaaa cgtgcgcccc atgacttgta tcaacgtgat ggtgtacgcg
     1861 gcttaaaatt gtatgtgcaa cgtgtgttta ttatggacga tgccgaacaa tttttgccaa
     1921 tgtacttacg ttttgtcaaa ggtattgttg actctaatga cttaccactc aacgtttctc
     1981 gtgagttatt acaatcaggc aaagttgttg atagcatgaa gtctgcttta acacgtcgtg
     2041 tgttagatat gttgaccagc atggccgaca gcaaagccga agactacgcg gtttgttggg
     2101 cgcagtttgg taatgtctta aaagaaggtg ttgctgaaga tcaaagtaac caagataaag
     2161 tcttaaaatt attaagattt gcctctacct tatcgggcga tgcggttgag tctgtggctt
     2221 tagccgatta tgtcgcgcgt atgaaagaag gccaagagaa aatttattat ctcaccgccg
     2281 aaaactttac tgccgctagc caaagtccac atttagaagt gttccgtaaa aaaggtgtag
     2341 aggtattgtt gttaacagac cgtttagacg aatggttaat gacccattta accgagtttg
     2401 atggcaaaca gttccagcat gttgctaaag gggacttgga cttaggcagt ttaagcagcg
     2461 aagaggacaa aaaagctgaa gaacaattgc aagagaccag tcagccttta gtggaacgtt
     2521 tgcaaaaaat cttagctgac aaggtgcaag cggtaaaagc tactcaacgc ttaacggatt
     2581 cgccagcttg tttggtgcgt agccaatatg atttaggtgc agccatgcgc caattattag
     2641 aggcaagtgg ccaaaagtta cctgagtcta aaccgacctt agaaattaac ccaagtcatc
     2701 ctttagtggc gcgtttaagc aacacgaatg atgacgcacg ttttgaaacc ttggccttaa
     2761 ttgtcttaga tcaagcaacc ttagcagaag gtagtgcact ggccgatcca gcggcttatg
     2821 tgaagcgttt aaatagctta ttgatggaat tagcgtaagc ttattatcaa ttgataaagg
     2881 gcgcgtatgc gccctttatt ttgcctaaat gcctaaagtt tgctatggtg gatagcagtg
     2941 attttaaatg ggaatagtta tgagcttatc taagctgcgt gttgctgtgt tgggtggggg
     3001 tagttttggt actgtcattg ccaatttagc agcaagtaat ggttgtcaaa ccacgcaatg
     3061 gttacgtgat gttgaattag ttgctgaaat gcaggctacc catcaaaata cccgttatct
     3121 taaaggtttt gatttacatc ccactttaca atttagcacc gatttagcgc aaacagtgag
     3181 tcaagcggat ttagtgtttg tggctattcc cagtaaagct tttagacagg tgattcaaac
     3241 ggcaaaacct cattttcaag ctaaacaaat gcttgtcagt atgactaaag gcattgaggc
     3301 agataccttt tcgttaatga gtcaaattat tcgtgaagaa gttcccaatg cacgtttagg
     3361 tgtgttaagt gggcctaatt tagccaaaga aattgctgcg aaaatgcctg caggtacggt
     3421 gattgctagt ttagaccccg atttacgtgc tactgttcat caagtgttag cttgcccata
     3481 cttta
//
LOCUS       DAOQMB010000244         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14399_length_3459_cov_36.192421, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000244 DAOQMB010000000
VERSION     DAOQMB010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14399_length_3459_cov_36.192421"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3392
                     /locus_tag="PLY05_08195"
     CDS             <1..3392
                     /locus_tag="PLY05_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNC03322.1"
                     /translation="ASKAIITHRFTSFDVLNDCFNEFNSLAASVGIDGRALLNKIDSF
                     NGQKINKHIALTKNDPVFAFVREYVFYWNKEPVGHYPVITLNTSKHGGQSETFNGLEA
                     ATARYKRALGLDDAKGLSRTKTAPKRDFEAEAKAHKAQAEKKAAQEAAYKAKRLKDFA
                     DVFNTLPREVGDHAYLVKKFGDLAPQAALSIDLRRGKDKLGDFVAYALHGQNGRIVGY
                     QRIYAEIPKGRDDNKDFIGSMGGAFAIVGDAEAVTGRAYIAEGLANAITYHLATGKPC
                     FVALNAGNLKNIVPFVRGNYADNITILADNDRKNLAQNGNTGINAALKAITYGGKQYD
                     YLIIAPCDNAVNWDLNDVLVSDGYNIEAVQAVVNNKENRRSVNIDPLGQRLESIELLP
                     RNQREKAVYALLAYAVDCAPIRPLDKSLERIKRVLGHEWSAFLESKALWISQKYNAKI
                     EAVKPLFSFKDNIAVHFAPQTMAKHTADTLFKNGGLYIDNRGTGSGKTLNMAEIVKLA
                     KYYKAQGERVGYISHRVSLSANSSARLALENYQELKPHDMPDVQYMAIVANSLAKWHF
                     AAFYEDVDVLFIDEIKQVLEHIAVGTFDNRERAKVLATIKKAIKNARLVICADADLDQ
                     TTLNFLKSCGKPIRRIASDISGNAPKIDKPVIYSDTKTIRDEAIRSAIAGNKLLIHCD
                     TKNETESLKLVLIASGLSESDILVVNSDTKAEPNEALFLKDPDAYLAQYKPRVVLASP
                     TIGSGFSIEKNYFDDVYMLLTGILTPTDIMQMSARYRPAKRLFIGFEDKNNRPEATSE
                     GAKLLGDIFINKRLKLAIDPMTDKLTIDVKASELDTLRYKILMQQEESRKDYANKTLL
                     CFIAKGHEVKRLSIEDANGSLSYKTASKQAKKAVKERRIVAIINSDVIDEQQAKQHEK
                     NANTTKLKEKLELARFKITDQLAINDPSPEDVKFWEDKGLKKIHLFETLRANEQACID
                     FDKQQAMTQENLSLRTKALEKHRIYTMIFDRLGIDKQTGKGSFSFTQALDTLKRLQVH
                     HNELAALGFGNFEKINERYAIRSITNLIKRLGLDTEAKQTRQGRTYSIKETSWQCMVI
                     YSDNRAKRAIHALKLSEAANVSPLANMTDDEILKNWGQKTA"
BASE COUNT         1172 a          712 c          744 g          831 t
ORIGIN      
        1 cagcaagcaa agcaattata acacatcgtt ttaccagttt tgatgtctta aacgactgct
       61 ttaatgagtt taactcgctt gccgcaagtg ttggcattga tggccgcgca ctgctaaaca
      121 agattgattc atttaatggt caaaaaataa ataaacatat cgcactcaca aaaaacgacc
      181 ctgtttttgc ttttgttcgt gagtatgttt tttattggaa caaagagcca gttggccatt
      241 atcccgttat cacattaaat acatcgaaac acggaggaca aagcgaaaca tttaacggat
      301 tagaagcagc aacagcgcgt tataagcgtg ctttgggctt agatgatgca aagggactaa
      361 gcagaacaaa gacagcacct aagcgcgatt ttgaggccga agctaaggcg cacaaagcac
      421 aagcagaaaa gaaagccgca caagaagccg cttacaaagc aaagcgttta aaagattttg
      481 ctgatgtttt taatacttta ccgcgtgaag ttggcgacca tgcttacctt gttaagaagt
      541 ttggagacct tgcaccacaa gccgcgttat ctattgacct tagacgggga aaagacaagc
      601 taggggattt tgttgcctat gctttacacg ggcaaaatgg ccgcattgtt ggctatcagc
      661 gaatttatgc cgaaataccc aaaggccgcg atgataacaa agattttatc ggttcaatgg
      721 ggggcgcatt tgccattgta ggcgatgccg aagcggttac aggccgcgca tatatagccg
      781 aaggtttagc aaacgccata acctaccacc tagcgacagg aaagccgtgt tttgttgcgt
      841 taaatgcagg aaacctaaag aacatcgtgc cgtttgttcg tggtaactat gccgacaata
      901 taacgatact ggcagacaac gacagaaaga accttgcaca aaatggcaat acaggcataa
      961 acgcggcact taaagcgatt acatacgggg gcaagcaata cgattactta attattgcgc
     1021 cttgtgataa tgccgtaaac tgggatttaa acgacgtttt agtgagtgat ggctacaata
     1081 tcgaagccgt tcaagccgtc gttaataata aagaaaaccg tagaagcgtg aacattgacc
     1141 cactagggca acgcttagaa agtatcgagt tgctaccccg taaccaacga gaaaaggccg
     1201 tttatgcact gttagcttat gccgtagatt gtgcgcctat acgcccacta gataagagtt
     1261 tagagcgtat caagcgcgtt ttaggccatg agtggagcgc gtttcttgag tctaaagcgt
     1321 tatggataag ccaaaaatat aacgccaaaa ttgaggccgt aaagccgtta tttagcttta
     1381 aggataatat cgccgttcac tttgcacctc agacaatggc caaacataca gccgatactt
     1441 tgtttaaaaa tggtgggcta tacatcgaca atcgcggtac aggttcaggc aaaaccttga
     1501 acatggccga aatagttaaa ctggcaaaat actataaagc gcaaggcgaa agagtaggct
     1561 atatatcgca tcgagtaagc ctaagcgcga acagctcggc acgactagcc ctagaaaact
     1621 atcaagagct aaaaccccat gatatgcccg atgttcagta tatggctatt gttgcgaact
     1681 ctttggctaa atggcacttt gccgcgtttt atgaagatgt cgacgtgctt tttattgatg
     1741 agattaagca agttttggaa catatagccg tcggtacttt cgacaatcga gaacgcgcca
     1801 aagtattggc aaccattaaa aaagccatta aaaacgcacg acttgttatt tgtgctgatg
     1861 ccgaccttga ccaaacgacc ttaaattttc tcaagtcatg cggcaaaccg attagacgta
     1921 tagcaagcga tataagcggc aacgcgccaa aaattgataa accagtcatt tactcagata
     1981 ctaaaactat tagagatgaa gccatacgtt cagcgattgc aggaaataaa ctactgattc
     2041 attgcgacac caaaaacgaa acagagagcc ttaaattggt attgattgcc tcaggactaa
     2101 gcgaaagcga tattttagta gtaaacagcg acaccaaagc agaaccaaac gaggcgttat
     2161 ttttaaaaga ccctgatgcc taccttgcac aatataagcc gcgtgttgtg ttagccagtc
     2221 cgaccattgg cagtggattt agcattgaaa aaaactactt tgatgatgtt tatatgcttt
     2281 tgactggcat tttaacgccc accgatataa tgcaaatgtc ggcacgttat cggcctgcta
     2341 aacgcttatt tattggtttt gaggataaga acaaccgacc agaggcaacg tccgaaggtg
     2401 caaaactatt aggcgatata tttataaaca aacgcttaaa actagccatt gacccaatga
     2461 cagacaaatt aacgattgat gttaaagcct cagagcttga caccttgcgt tataaaatac
     2521 ttatgcaaca ggaagaaagc cgaaaggact acgctaataa aacattgctt tgctttattg
     2581 ctaaaggcca tgaagtgaaa cgactcagca ttgaagatgc aaacggttca cttagttata
     2641 aaacagcatc gaagcaagcc aagaaagccg ttaaagaacg tcgtattgtt gcaattatta
     2701 attccgacgt aatcgacgaa cagcaagcca agcagcacga aaagaacgcg aacacgacca
     2761 agttaaaaga aaagttagaa ctggcacgtt ttaaaataac agaccagtta gcaattaatg
     2821 accctagccc cgaagatgtt aaattttggg aagataaagg cttaaagaaa attcatttat
     2881 tcgagacttt acgggcaaac gaacaagcct gtattgactt tgataagcaa caggcaatga
     2941 ctcaagaaaa cttatcattg cggacaaaag cactcgaaaa gcatcgaatt tacacgatga
     3001 tttttgacag actggggatt gataagcaaa cgggcaaagg ttcgtttagc tttactcagg
     3061 cattagacac acttaaaagg ttacaggttc accataacga actagccgcg cttggttttg
     3121 gtaactttga gaaaataaac gaacggtacg ccattcgctc aattacaaac ctgattaaac
     3181 gactcggact agacaccgaa gcaaaacaga cccgacaagg ccgcacttat tcgattaaag
     3241 aaacaagttg gcaatgtatg gttatttact cagacaatcg agcaaagcga gctattcacg
     3301 cgctaaaact aagcgaggcc gcgaacgtgt caccacttgc gaacatgacc gatgacgaaa
     3361 tactaaagaa ttgggggcaa aaaacagcat aatgccaaca gataaaatac tattgtctaa
     3421 gcaaaagtaa attctatgtg ttggcaaaca gcataaacc
//
LOCUS       DAOQMB010000245         3455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14428_length_3455_cov_20.265588, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000245 DAOQMB010000000
VERSION     DAOQMB010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14428_length_3455_cov_20.265588"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2234)
                     /locus_tag="PLY05_08200"
     CDS             complement(<1..2234)
                     /locus_tag="PLY05_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNC03323.1"
                     /translation="MQIYSKDGKLISEFGEKHSRPLHYNEIPPLLVKAFLAAEDDTFF
                     VHQGISLKGLARAFVDLAESGSIQSGGSTITMQVAKNYFLSSQRTFSRKFTEILLAQN
                     IEQALTKQEIFELYVNKIYLGQRAYGIGAAAEVYYNKKNIHTLSLAEMAMIAGLPKAP
                     SKYNPVVNPKRAIERRNWILGRMLKLGFINKAAHDQAIQEGTGIEYRSEVADVSAPYL
                     AEMVRAALTARFGETVLGSGYKVYTTVRSDIQNAAVQSVIDGLMAYDLRHGWRGAEAN
                     SEDKPLSHFDVVGGLSPAQVLKVNKHSVEALMRDGQTVTIPWGGLSWARAYKSVNSVG
                     PSPNKASQIVKVGDIIRIKKAGGIWVLRQIPKVQGELIALNPETGAIEALAGGFDFNI
                     SQFNHAIQGWRQAGSTLKPFVYALALERGFTPYSVVSDSPMTIGNWSPSNADGRFMGP
                     ITLRRALYLSRNLVSVRLLQSVTLDRARQYLPRFGFIDDKLPNNLTLALGTAQVVPLQ
                     MATAYAAFANGGYRVNPYFIERIEDTKGTVLFQAKPEQVCKPCENPALANEMMTPRPE
                     TPPSEIVGQGESVKVIEAPSEENQLTAPSTTLTLPDYPIAKRIMSEKASKDMANILRD
                     VILHGTARSALRLGRSDIAGKTGTTNDAKDAWFAGFNPKLVAVSWVGFDQPASLGRLE
                     YGGYAALPLWNSFMDYALADIPEQWIDGSRPQAAKPKANTANNSSSAPSDASPTTEPQ
                     NNSET"
     gene            2575..>3455
                     /locus_tag="PLY05_08205"
     CDS             2575..>3455
                     /locus_tag="PLY05_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNC03324.1"
                     /translation="MPFLSKKNTSLLGLDISSTSVKLLELSLQGERYRVESYGVEPLP
                     QNAVVEKNIADVEGVGDAITRLIAKAKPKTKNVAVAVSGSAVITKTIEMDADLSDDER
                     ESQIRLEADQYIPYPLEEISLDFEVIGPSTSNPERVSVLLAASRTENIELRTDTLVIA
                     NLTAKIVDVEAYAMERAFDLLVDSLPCGRDGTVAIIDIGASMTTLSVVNDGKIIYTRE
                     QMFGGKQLTEEIQRRYGLSFEEAGRLKKEGGLPDDYEVEVLRPFKEAVVQQVTRSLQF
                     FFSSSQYNDVDHILLAGG"
BASE COUNT         1024 a          672 c          734 g         1025 t
ORIGIN      
        1 gtttcgctat tattttgcgg ttcagttgtt ggactagcgt cacttggtgc agaactgcta
       61 ttgttagctg tattggcctt cggtttggct gcttgtggac gagaaccatc aatccattgt
      121 tctggaatat ctgctaaggc ataatccata aatgagttcc ataagggtag agctgcataa
      181 ccgccatatt ctaaacgtcc taatgaggca ggttgatcaa aaccaaccca actgactgca
      241 actagtttgg gattaaaacc tgcaaaccat gcatctttag catcatttgt cgttcctgtt
      301 tttcctgcaa tatcactacg tcctaagcgc aatgcgctac gtgcagttcc atgtaaaatg
      361 acatcacgta agatattagc catatctttg gatgctttct cactcataat acgtttagca
      421 ataggatagt ctggtaatgt taaagtggta cttggtgcag tgagttgatt ttcttctgag
      481 ggggcttcaa tcactttaac ggattcccct tgaccaacaa tttcgctagg tggagtctct
      541 ggacgaggtg tcatcatttc attggcgagt gcaggatttt cacacggctt acaaacttgc
      601 tcaggttttg cctgaaataa aacagtgcct ttagtgtctt caattcgctc aataaagtaa
      661 ggatttacac gataaccacc atttgcaaag gctgcatagg cagtggccat ctgtaaggga
      721 acaacctgtg ccgtccctaa tgctaaggta agattattcg gtaatttgtc atcaataaaa
      781 ccaaaacgag gcagatactg acgtgctctg tctaaagtaa cggactgtaa taaacgtaca
      841 gacaccaaat tacgggataa atacaaagct cgtcttaaag taatgggacc cataaagcga
      901 ccgtcagcat tactaggact ccaattacca attgtcatag gtgagtcact gacaacactg
      961 taaggtgtaa atcctcgctc taatgccaac gcataaacaa acggtttgag ggtagagcca
     1021 gcttgacgcc aaccttggat agcatgatta aattggctga tattaaaatc aaatccccct
     1081 gcaagtgcct caattgctcc tgtttctggg ttgagtgcaa ttagttctcc ttgtactttg
     1141 ggaatttgac gtaaaaccca aattcctcct gcttttttga tgcgaataat atcgccaact
     1201 ttaacaattt gactggcttt attaggcgaa gggcctacgc tattaaccga tttataggct
     1261 cttgcccaac tcaatccacc ccatggaata gtgactgttt gaccgtcacg catgagagct
     1321 tcaacagagt gtttattaac ttttagcact tgagcaggcg atagaccgcc aacaacatca
     1381 aaatgagaaa ggggtttatc ttcgctattg gcttctgccc cgcgccaacc atgccgtaaa
     1441 tcataagcca ttaaaccatc aattacggat tgaaccgctg cattttgaat atcggagcgg
     1501 acggttgtat aaactttata accactgcct aatactgttt cgccaaagcg agccgttaaa
     1561 gccgcacgca ccatttcagc aagataaggt gctgatacgt ctgcaacttc actccgatat
     1621 tcaatccctg ttccttcttg aatagcttga tcgtgagccg ctttgttaat aaagcctaac
     1681 tttaacatac gccccaaaat ccaattacgc cgctctatgg cacgtttagg attaacgacg
     1741 ggattatatt ttgaaggggc tttaggcaga cctgcaatca tcgccatttc tgctaaagaa
     1801 agagtgtgaa tatttttttt attgtagtaa acttcggcag cagcaccaat gccataagca
     1861 cgttggccaa gataaatttt attgacgtaa agctcaaaaa tttcttgttt agttaacgct
     1921 tgctcaatat tttgtgctaa aagaatttcg gtaaatttac ggctaaatgt acgctgtgat
     1981 gataaaaaat aatttttcgc aacctgcata gtaattgttg acccacctga ttgaatagag
     2041 ccactttcgg ctaaatcaac aaatgcacgt gcgagacctt ttaaactaat gccctgatga
     2101 acaaaaaaag tatcatcttc agcagctaaa aaggctttga ctaataaagg cggaatttca
     2161 ttgtaatgaa gagggcgact atgcttttcg ccaaattcac tgatgagttt tccgtcttta
     2221 ctgtaaattt gcaaaggggt ttccagttta atttcgcgga gctgttcaac atttggcaat
     2281 tgtggtgtaa ggtataaata gacaccgctg ctaagtattg atacgctaag cagacctaat
     2341 aagccgaaag cgaccaatgg atgaaaattg attgatttaa gatttatttt gttcattgga
     2401 aaccaaggtt aataacaaac taggtgacag tcactaattt atggtagatt atacctatat
     2461 aagtacttgt gcggtacttg tagtcgtgtt aagactatca tttaatgtta attggcaaga
     2521 atgtggtata actctgcatt cttattacaa aaataaaagg gaacaaacaa cgtgctgccc
     2581 tttctcagta aaaaaaatac aagcctattg ggcttagaca ttagttctac atccgtcaaa
     2641 ctcttggagc ttagtctaca aggcgagcga tatcgtgtgg aaagttatgg ggtcgagcca
     2701 ctgccgcaaa atgctgtggt tgagaaaaat attgctgatg ttgaaggtgt gggtgatgca
     2761 attactcgat tgattgctaa agctaagcct aaaacaaaga atgtagctgt cgcagtttca
     2821 ggttcagcag ttattactaa aacaattgag atggatgctg atttgtccga tgatgagcgt
     2881 gagtctcaaa ttcgtttaga agctgatcag tatatcccat acccattgga agaaattagc
     2941 ctagattttg aagtgattgg gccatcaaca agtaatccag agcgggtgag tgttttgttg
     3001 gcagcttctc gtacagaaaa tatagaattg cgtactgata cattggtaat tgcaaattta
     3061 accgcaaaaa tagtcgatgt tgaagcgtat gccatggaac gtgcattcga tttattagtg
     3121 gacagtttgc cgtgtggtcg tgatggtact gttgctatta tagatattgg ggcatcaatg
     3181 actacactga gtgttgttaa cgatggtaaa attatttaca cgcgtgaaca aatgtttggt
     3241 ggaaagcaat taacagaaga aattcaacgg cgttatggtt tatcttttga ggaagcggga
     3301 cgattaaaaa aagaaggtgg cctccccgac gattatgaag tagaggtatt gcgccctttt
     3361 aaagaggcgg ttgtccagca agtgacacgc tctttgcagt ttttcttttc ttcaagtcaa
     3421 tataatgatg ttgatcatat attattagca ggtgg
//
LOCUS       DAOQMB010000246         3454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14438_length_3454_cov_9.726979, whole genome shotgun sequence.
ACCESSION   DAOQMB010000246 DAOQMB010000000
VERSION     DAOQMB010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14438_length_3454_cov_9.726979"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /gene="narH"
                     /locus_tag="PLY05_08210"
     CDS             <1..1438
                     /gene="narH"
                     /locus_tag="PLY05_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HNC03325.1"
                     /translation="WTSRQGMEYAWFNNVETKPGIGYPDDWENQERWNGGWERLSNGK
                     IRPKLGAKWRVLANIFANPDLPQIDDYYEPFTFDYSHLQQAPESKVTPTARPVSTITG
                     KVMDKIEKGPNWEEILGGEFSKRSADYNFQEVQKEIYGQFENTFMMYLPRLCEHCLNP
                     ACVAACPSAAIYKREEDGIVLIDQDKCRGWRMCVSACPYKKIYFNWESGKSEKCVFCY
                     PRIEQGEPTVCSETCVGRIRYLGVLLYDADLIEQAASTENEGDLYQAQLNMFLDPNDP
                     QVIAAAKEEGIPDSWMEAARRSPVYKMAIDWKIAFPLHPEYRTLPMVWYVPPLSPIQN
                     AHAKGQMGTNGVIPDVSSLRIPLRYLANLLTGGNEAPVKLALERLLAMRVYKREQQVY
                     QQKNYLVLRQTGLNEAMVEEMYRYLAIANYEDRYVIPSSHREYAGRAFDVRSSCGFSF
                     GNGCSNGDNKVDLFGKQRKANLWGGTHE"
     gene            1431..2084
                     /gene="narJ"
                     /locus_tag="PLY05_08215"
     CDS             1431..2084
                     /gene="narJ"
                     /locus_tag="PLY05_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HNC03326.1"
                     /translation="MNKPSLIFSALSHLLLYPDDVMRQQLSEVRDILREERRLSEATR
                     RALLAFAEDLQQGDGWDVQEMYVNLFDRTRSLSLHIFEHVHGESRDRGQAMVDLINLY
                     QEHGLVLQAENRELPDYVPLFLEFLSTLETDAAHDLLKEPLHVLQAIGARLRQRDSAY
                     GLIFSALEELAGARSLIATENDEEQDIDEAWQDAEVRFLDAATPSCRPQTATAMRGE"
     gene            2086..2781
                     /gene="narI"
                     /locus_tag="PLY05_08220"
     CDS             2086..2781
                     /gene="narI"
                     /locus_tag="PLY05_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HNC03327.1"
                     /translation="MEHLANWINTLFFGIYPYIAFAVFAVGCVVRFDREQYSWKASSS
                     QLLDKSSLRLGSNLFHIGVLFIIVGHLVGLLTPTSIYTQFITVPQKQLLAMVAGGIFG
                     ALCFTGLLLLITRRLNNPRLREHTRFSDWLVLWMLFIQVSLGLSTLFVSAQHLDGGSM
                     LNLSHWAQHIVTFQPNAADFIKDEHWLFKVHIWLGLSLFVVAPFSRLVHVLSAPIWYV
                     FRPYQVVRSRWTR"
     gene            2794..3258
                     /locus_tag="PLY05_08225"
     CDS             2794..3258
                     /locus_tag="PLY05_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03328.1"
                     /translation="MQQDVYVNHKLIPEDMIGQEMQYHPAQSKQEAWQKAAEALVLRE
                     LLLQEAFKEHLTDVDNDEAEIIDALLAKKIDIQEPSQEECLAFYQAQKHRLLDSAGQI
                     IPYSQLELLIKTELAARTGRLAVKDYLQTLVEQSQIQGLVIGQTWLPVRMMN"
     gene            3274..>3454
                     /locus_tag="PLY05_08230"
     CDS             3274..>3454
                     /locus_tag="PLY05_08230"
                     /inference="COORDINATES: protein motif:HMM:NF016303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HNC03329.1"
                     /translation="MDYLHMPVGQIAAKLSGSTAVFQKYGIDFCCGGKQVLNDVLSKK
                     RLDAPTVLRELIALEK"
BASE COUNT          973 a          635 c          802 g         1044 t
ORIGIN      
        1 gtggacatca cgccaaggga tggaatacgc gtggtttaac aacgtcgaaa ccaaaccagg
       61 tattggttat cctgacgatt gggaaaatca agagcgttgg aatggcggct gggagcgttt
      121 aagtaatggc aaaattcgcc ctaaattggg tgcaaaatgg cgagtattag cgaatatttt
      181 tgccaatcct gacttgcctc aaatagacga ttattacgag ccatttacct ttgattatag
      241 tcatttgcag caagcacccg aatccaaagt cacgccaacg gcgcgtcctg tttcgaccat
      301 tactggcaaa gtcatggata aaatcgaaaa agggccaaat tgggaagaaa ttttaggcgg
      361 agaatttagt aaacgctcgg cagactataa ttttcaagag gttcaaaaag agatttatgg
      421 tcaatttgaa aatacattta tgatgtattt accacgttta tgcgagcatt gtcttaatcc
      481 tgcttgtgtg gctgcttgtc caagtgcggc tatttataaa cgtgaagaag acggcattgt
      541 tttaatcgac caagataaat gtcgtggttg gcggatgtgt gtatcagcct gcccatacaa
      601 aaagatttat tttaattggg agtcgggtaa gtccgaaaaa tgcgtatttt gttatccacg
      661 cattgaacaa ggtgaaccaa cagtttgctc cgaaacgtgt gtggggcgta ttcgttattt
      721 aggcgtatta ttgtatgacg ctgatttaat tgagcaagct gctagcaccg aaaacgaagg
      781 cgatttatat caagcccaat taaatatgtt tttagacccc aatgacccac aagtcattgc
      841 cgctgcaaaa gaagaaggta ttcctgattc ttggatggaa gcggctcgtc gctcacctgt
      901 ctacaaaatg gcgattgatt ggaaaatcgc ttttccttta catccagaat atcgcacctt
      961 gcccatggtt tggtacgttc caccattatc gcctattcaa aatgcccatg ctaaaggaca
     1021 aatgggcaca aatggtgtga ttcctgatgt gtcgtcattg cgtattccct tgcgttactt
     1081 agctaattta ttaacaggag gtaacgaagc acccgttaaa ttggctttag aaagattgtt
     1141 agcgatgcgt gtctataaac gtgagcagca agtctatcaa caaaaaaact atcttgtatt
     1201 gcgtcaaaca gggcttaacg aagcgatggt tgaggaaatg taccgttatt tagccattgc
     1261 caattacgaa gaccgttatg tgattcctag ctcacatcgt gaatatgcag gcagagcgtt
     1321 tgatgtgcgt tcaagttgtg gttttagctt tggcaatggt tgctctaatg gtgacaataa
     1381 agtcgatttg tttggcaaac aacgcaaagc caatttgtgg ggaggcacac atgaataagc
     1441 ccagcctaat ttttagtgca ttaagccatt tattacttta tccagatgat gtgatgcgtc
     1501 aacaacttag cgaagtgcgg gacattctgc gtgaagaacg acgtttatcc gaagctacgc
     1561 gccgtgcttt attagccttt gccgaggatt tacaacaagg ggatggttgg gacgtgcaag
     1621 agatgtatgt caatttgttt gatagaacac gctcattatc gttgcatatt tttgaacacg
     1681 ttcatggcga atcgcgtgat agaggtcaag cgatggtgga cttaatcaat ctttatcaag
     1741 agcatggttt ggtgttacag gccgaaaacc gtgagttacc agactatgta ccgttgtttt
     1801 tagagttttt atcaacctta gaaaccgatg ctgctcatga tttgctcaaa gagccactcc
     1861 atgttttaca agccataggc gcgcgtttac gtcaacgtga ttcggcgtat ggtttgatat
     1921 ttagtgcctt agaggagcta gcaggtgcgc gttctttaat tgccaccgaa aacgatgaag
     1981 aacaagacat tgatgaagca tggcaagacg ccgaagtgcg ctttttagac gcagcaacgc
     2041 cttcttgtcg tccacaaacc gcaacagcaa tgcgaggtga ataacatgga acatttagca
     2101 aattggataa ataccctatt ttttggcatt tatccttata tcgcctttgc cgtttttgcg
     2161 gtgggttgtg tggttcgttt tgaccgtgag caatattcgt ggaaagccag ctcatctcag
     2221 ttattagata aaagcagttt acgcttaggc agtaatttat ttcatattgg tgtgctgttt
     2281 attattgtgg ggcatttagt aggcttgtta acccccacca gtatttatac tcaatttatt
     2341 accgtgccac aaaaacaact attggcaatg gtggcaggcg gcatttttgg cgcattgtgt
     2401 tttacaggtt tattgttgct cattactcgt cgtttaaaca atccacgttt acgcgagcat
     2461 actcgttttt cagattggct agtgttgtgg atgttgttta ttcaagtgtc tttgggttta
     2521 agcactttgt ttgtttctgc tcaacattta gatggtggca gtatgcttaa tttatcgcat
     2581 tgggcgcagc atattgtcac tttccaaccc aatgcggctg attttatcaa agatgaacat
     2641 tggctgttta aggtgcatat ttggttagga ttatcgttat ttgttgttgc accgtttagt
     2701 cgtttagttc atgtgttaag tgcgccaatt tggtatgtgt ttagaccata tcaagtggtg
     2761 agaagtcgtt ggactcgtta agagaggtgt cacatgcagc aagatgttta tgttaatcat
     2821 aaattaattc ctgaagatat gattggccaa gaaatgcagt atcaccctgc acaaagcaaa
     2881 caagaagcat ggcaaaaagc cgctgaagcc ttggtattgc gcgaattgtt attacaagag
     2941 gcttttaaag agcatttaac cgatgttgat aacgatgaag ccgagattat tgatgcttta
     3001 ttggcaaaaa aaatagatat ccaagaacca agccaagagg agtgtttggc tttttatcaa
     3061 gcccaaaaac atagattact cgacagtgca gggcaaatta ttccctattc gcagttagag
     3121 ctgctgatta aaaccgaatt agctgctcga acgggtcgtt tggctgttaa agactacttg
     3181 cagactttag ttgagcaaag ccaaattcaa ggccttgtta ttggtcagac ttggttacct
     3241 gtgcgtatga tgaactaatt tggggagaca aacatggatt atttacatat gcccgtcggt
     3301 cagattgccg ctaaattatc tggctcaacc gctgtatttc aaaaatatgg tattgatttt
     3361 tgttgtggcg gtaaacaagt gctaaatgac gtgttaagca aaaaacgatt agatgcacca
     3421 actgtgttac gagagttaat tgcgctagaa aaaa
//
LOCUS       DAOQMB010000247         3450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14478_length_3450_cov_14.329013, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000247 DAOQMB010000000
VERSION     DAOQMB010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14478_length_3450_cov_14.329013"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1411)
                     /locus_tag="PLY05_08235"
     CDS             complement(155..1411)
                     /locus_tag="PLY05_08235"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNC03330.1"
                     /translation="MSKFKPASAFDSNTPSAAPTTTAPVVPEPNFSSSTNHALVTAPE
                     HTPVVYQAPTQPASYLDMVLAKHEQRLQQEGSDVNKIRAEVAKIAQSFDPRRADAIHT
                     LGSQSGAKVVQYSDKLLSQVKSKDLEGLGDNLNEVVLLAKGINISSLVDNSKSKIPLI
                     GGLIDSFKMNKEKVLGRYESLSKQIEKLVKEMQGSQVRLANRIQDLEKVYTFNVEEYH
                     TLECYILVGEVKIEELRAEIAERQAAPAATNPMEAQAISNLQDVLNRLEKRVHDLRTM
                     QMVAVQTAPMIRMVQSNNQLLIDKFRNLQELTIPSWKKQFTLAVALIEQQKAVELTQK
                     IDDTTNDLMRRNAELLKQNSVNTARANQRAVVDIETLEYVQQTLISTIEEVEQIQRDG
                     EQARADAVIKMESMKGELITKLGGRT"
     gene            complement(1408..>1838)
                     /locus_tag="PLY05_08240"
     CDS             complement(1408..>1838)
                     /locus_tag="PLY05_08240"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNC03331.1"
                     /translation="FKDLTQYLLAGQLRLEEEQQLMQQLMQQQNPVAANVFAQQALID
                     KQNALSRFERRLQTLKVLRHTVLLRIGQLRLEQQNTLTLIDQANETLNLVVPAWKQQI
                     LALFSLSSSDNNSLLYQQLADTQQSLHQKLLSLNETKERS"
     assembly_gap    1839..1938
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1939..2633)
                     /locus_tag="PLY05_08245"
     CDS             complement(<1939..2633)
                     /locus_tag="PLY05_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNC03332.1"
                     /translation="MVKTFHQKNVEKNQQMTTSSPVIVTDTEITPKTFHGKQSTVMDN
                     NQPKPLFKPNVQATADEQSLQQVQGSIARLQLDDYEFSASDIAKATLLKAAIRIDDPS
                     YVTDYGAEACQQSETILNQLNTISRNDYAEGVRKYLGYILTDTQKVDIEAISKGGEKS
                     FFSRLFSNGISTKSDFLGLERDIKSNMTFCQNRLNQLKKTQQIFSELFAKNEQQFKDL
                     TQYLLAGQLRLEE"
     gene            complement(2644..>3450)
                     /locus_tag="PLY05_08250"
     CDS             complement(2644..>3450)
                     /locus_tag="PLY05_08250"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNC03333.1"
                     /translation="GLIIGSIAAWFPHGPAWVIRRNLKLCFPEQSERWVNDTTKLNLI
                     YTAQTALEFTKTWGMPPEYSLSQMTEVENMEIFTDAVKSPKGCFILVPHHGNWEFMNA
                     WVSSHCSPVIMYKPSKNKAMDLFVRQARSRLNSTLVPTDESGVKAVFKALKSGGVSII
                     LPDHTPHDNGGMFAPFFGTSVWTGILASKLIQKTQCEVIMLSCLHHPSGKGFKITVDK
                     VEPDVYSKDLLTSVSAVNKSVENLIRRNPAQYQWVYKRFKKCEHLKNVYQ"
BASE COUNT          920 a          647 c          672 g         1111 t
ORIGIN      
        1 cttaaattaa tcgattcgcc tttttttaag ttaatagcca tgtttttgct cctaatgaaa
       61 tttgttgaga atattaatta tcccttcgac tccgctcagg gaacagagcg gtggttgagc
      121 ttggccagcc ctgagcctgt cgaaggggcg aaacctaagt tcgaccacct aatttagtaa
      181 ttaattcgcc tttcatgctt tccattttaa tcacagcatc agcacgtgct tgttcaccat
      241 cacgttgaat ttgttcaact tcttcaatgg tagaaattaa ggtttgttgc acatattcaa
      301 gggtttcaat atcaactact gcgcgttgat tagcacgagc tgtatttacc gagttttgtt
      361 ttaataattc agcattacga cgcattaaat cgttggtggt atcatcaatt ttttgtgtca
      421 attcaacggc tttttgctgt tcaatcaaag caaccgctaa ggtaaattgt tttttccaag
      481 aggggatagt gagctcttgt aaattgcgga atttatcaat caataactga ttgttgctct
      541 gaaccatacg aatcatcggt gcagtttgta cggcaaccat ttgcatggta cgtaaatcgt
      601 gaacccgttt ttctaaacga ttgagtacat cctgcaagtt agaaatcgct tgagcttcca
      661 tcggattagt agcggcaggc gcggcttgac gctcagcaat ttcggcgcgt aactcttcga
      721 ttttgacttc acccactaaa atataacatt caagggtgtg atattcttca acgttgaagg
      781 tataaacctt ttctaaatcc tgaatacgat tggctaagcg tacttgtgag ccttgcattt
      841 ctttgacaag tttttcaatt tgtttgctta aactctcata acgacctaaa actttctctt
      901 tattcatttt aaaagagtca attaagccgc caattaacgg aattttggat ttagaattat
      961 caactaagct agaaatatta atacctttgg ccaataacac gacttcattt aagttatcgc
     1021 ccaaaccttc taaatcttta cttttaactt gcgataataa cttgtcagag tattgcacca
     1081 cttttgcacc agattgtgag cctaatgtgt gaatggcatc agcacggcga gggtcaaaac
     1141 tttgggcaat tttagccact tcagcccgaa ttttattaac gtctgagcct tcttgttgca
     1201 aacgttgttc atgtttggcc agtaccatat caaggtaaga tgcaggctgt gtgggtgctt
     1261 gataaaccac aggcgtgtgt tcaggggcag tgactaaagc atgattagtg ctactgctaa
     1321 aattgggttc aggcacaaca ggggcagtgg ttgttggggc agcagaaggg gtattgctgt
     1381 caaaagcaga agcaggttta aatttgctca tgatctttcc ttagtctcat ttaaagagag
     1441 tagtttttgg tgtaatgatt gttgtgtgtc agcaagttgt tgatataaca aactgttatt
     1501 atcactacta gagagtgaga ataaagctaa gatttgttgt ttccaagcag gcaccactaa
     1561 atttagggtt tcattcgctt gatcaattaa ggttagcgtg ttttgttgtt ctaaacgtag
     1621 ttgaccaata cgtaataaca ccgtatgtcg taaaaccttt agcgtttgca atcttcgttc
     1681 aaagcgagac agggcatttt gtttgtcaat taatgcttgt tgcgcaaaga cattagccgc
     1741 cacaggattc tgttgttgca tcagttgttg catcagttgt tgctcttctt ccaaacggag
     1801 ttgacccgct aacaaatact gggttaagtc tttaaattnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnntc ttcttccaaa cggagttgac ccgctaacaa atactgggtt
     1981 aagtctttaa attgctgctc atttttggca aataattcgc taaaaatttg ttgggtcttt
     2041 ttgagttggt ttaagcgatt ttggcaaaac gtcatattac ttttaatatc acgttctaag
     2101 cctaaaaagt ccgattttgt gctgatgcca ttgctaaaca aacgcgaaaa aaaagatttt
     2161 tctccgcctt tactaatcgc ttcaatatcc actttttggg tgtcggttaa aatatagccc
     2221 aaatatttac gcacgccttc ggcataatca ttacgcgaaa tagtattgag ttgattgagg
     2281 atggtttcac tttgttggca agcttctgcg ccataatcgg tgacataact cggatcatca
     2341 atgcgaatag cggcttttag caaagtcgct ttggcgatgt cactggctga aaactcatag
     2401 tcatctaatt gtagacgggc aattgagcct tgtacttgtt gtaggctttg ctcatcagcc
     2461 gttgcttgca catttggttt aaataaaggt ttgggttgat tgttgtccat tactgttgat
     2521 tgcttaccat gaaaagtctt gggagtaatt tcggtatccg tcactataac aggtgaagag
     2581 gtggtcattt gttggttttt ttcaacattt ttttgatgaa aggttttgac caccgatgat
     2641 ctcctattga taaacgtttt ttaaatgctc acatttttta aaacgtttat atacccattg
     2701 atattgggct ggattgcgcc gaatcaagtt ttcaacactt ttgtttaccg cagaaaccga
     2761 cgttaataaa tctttgctat aaacatcagg ttctacttta tcaacggtaa ttttaaaacc
     2821 ttttcctgaa ggatgatgta aacacgataa cataatcact tcacattgag tcttttgaat
     2881 cagtttagac gctaaaatgc ctgtccacac cgaggttcca aaaaatggag caaacatccc
     2941 accattatca tgtggggtat ggtcgggtaa aataattgac acaccacctg attttagggc
     3001 tttaaacacc gcttttacac cactttcatc agtcggaaca agtgtgctat ttaaacggct
     3061 ccgtgcttga cggacaaaaa gatccatcgc tttgttttta ctcggtttgt acataatcac
     3121 ggggctacaa tgagagctta cccatgcatt cataaattcc caattgccat gatgcggcac
     3181 taaaataaaa cagccttttg gtgattttac tgcgtctgta aaaatctcca tattttcgac
     3241 ttcggtcatt tgacttaggc tgtattcggg tggcatcccc catgttttag taaactctaa
     3301 ggcggtttgt gcggtataaa ttaaattgag tttagttgtg tcattcaccc aacgttcact
     3361 ttgttcggga aagcataatt taagattacg acgaattacc caagcagggc catgtggaaa
     3421 ccatgcggca atactgccaa tgattaagcc
//
LOCUS       DAOQMB010000248         3393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_14925_length_3393_cov_6.090773, whole genome shotgun sequence.
ACCESSION   DAOQMB010000248 DAOQMB010000000
VERSION     DAOQMB010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14925_length_3393_cov_6.090773"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PLY05_08255"
     CDS             <1..879
                     /locus_tag="PLY05_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HNC03334.1"
                     /translation="LQAGRSSVDRWSTWYEVGGVDGLKTKGVGRPRRQPKSLICGVLK
                     ILLDYTPRTFGFQRSRWSSELFSLLLNKLCKVKVHSSTIRKWLPEIGIVWRRAAPTLY
                     IQDPEKDVKLAAIFKALENCSADHPVFYQDEVDIHLNPKIGADWGYRGKQRKVATPGQ
                     NKKHFLAGALHSQTGQISYVKGNRKTTDLFLELLKKLKRQYRRAKTITLIVDNYIIHK
                     SKKAQKWLSENEKFKLLFLPVYSPWHNKIEKLWHALHETITRNHQCKNMDELLEQVYY
                     FMDTVAPFPGGRHGLK"
     gene            905..1258
                     /locus_tag="PLY05_08260"
                     /pseudo
     CDS             905..1258
                     /locus_tag="PLY05_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1219..1920)
                     /locus_tag="PLY05_08265"
     CDS             complement(1219..1920)
                     /locus_tag="PLY05_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03335.1"
                     /translation="MKPTEEQIAAINSALEGITFKISAYAGSGKTSTLKLIGNTLSDQ
                     QGIYLAFNKAIADDAKKRFPQNVKCQTFHSLAFKQTPDYIKEKLNFNRLLPRKMAEML
                     CLQDYRVPLRKYHSASLTTKEDHHDSLATTDSFKESYAEYRTCTSYDQAMILNRAIGL
                     YCRSKELTVTPAIIIKAMPQWIDEADCRDLIGNLATCANILWKKLIDEHTPIKITHIV
                     EPLLTFTNSSAKFRC"
     gene            complement(1999..>3393)
                     /locus_tag="PLY05_08270"
     CDS             complement(1999..>3393)
                     /locus_tag="PLY05_08270"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNC03336.1"
                     /translation="VANSSLKLNASKTMEIAQSLFESGHITYHRTDNPNMSAEGFDAA
                     SQWLTSKGFTPNNTPHKWPAKDNAQGAHEAIRPTNFNNIVVDSNSQVQQLYALIHERA
                     VASCMPSAIDEVTTIEACSENHYTFNEEQIKALFKAVASVELVKGWRQLMVLDSPDDK
                     ESLGKIQKVPRVGDIHGVIASKIDSKMTAPPPRYTEGSLIKKLEALGIGRPSTYATIV
                     QNIISKSYIQVGSPDNKDKNKQSLFPTELGIAIIDALKQQKFLDYDFTANLEDELDEI
                     ANSATTYNQVISQLYDVIQQDITAIKLDRGLLPLGEFEQACPKCSKAIKRLQKKDKSG
                     HFWVHIEEADAEGCERFIADNRGKPVKLESLGQPCPKCEKAIKRVQKRDKSGFFWVHV
                     DEVDANNCERFIPDNRGKPTKLESVNQPCPKCTKLIKRLQKKDKSGYFWVHIEEADAE
                     GCGRFIADNQGKPI"
BASE COUNT          924 a          679 c          698 g         1092 t
ORIGIN      
        1 ctgcaggctg gacgttcgtc ggttgaccga tggtcaacct ggtatgaagt cggcggcgtc
       61 gatggcctta aaaccaaagg tgtgggtcga ccgcgtcgac agccaaaaag tttgatttgc
      121 ggtgtcctaa aaatcttgtt ggactacacg ccgaggactt ttggttttca gcgttcgcgc
      181 tggagttctg agctgttttc tttattactt aataagttgt gtaaggtaaa agttcatagc
      241 tctacgataa ggaaatggtt gcctgaaatc ggcatcgtat ggcgtcgggc agcgccaacc
      301 ctctacattc aagacccgga gaaggatgta aagttagccg caatcttcaa agcacttgaa
      361 aactgtagtg cggaccatcc ggttttttat caggatgaag tggatattca cctgaacccc
      421 aagattggcg cggactgggg ttacagagga aagcagcgca aagtggccac gccaggtcag
      481 aacaaaaagc attttctggc cggagcgctg catagtcaga ctggccaaat cagttatgtt
      541 aaaggcaatc gtaaaaccac cgatttgttt ttagagctgc taaaaaaact aaaaaggcag
      601 taccggcgcg ccaaaacaat tacgctcatt gtggacaact acatcatcca caagagtaaa
      661 aaagcacaga agtggctgag cgaaaacgag aaattcaagc tgctgttttt accggtctat
      721 agtccgtggc ataacaaaat agaaaaactc tggcatgcgc tgcatgaaac gatcacccgt
      781 aatcatcagt gcaaaaacat ggatgaatta ctggaacagg tttactactt tatggacaca
      841 gtggcgccat ttccgggtgg gcggcatggg ttgaagtagg tgtatcacaa ttaggatcag
      901 ttatttagcc gatcgtattc gaaggcactt gcgcccacta tataccacga tcaacttctc
      961 ttgtaataca actaccagcc catggctttc tgcgttagca tggatgaaag acgttttttc
     1021 taagcaacaa cgtttatctc aacgaccgct acatgaatgt attgaagaca ctgtcccgca
     1081 acgattgcga ccctacctgc tcacctttga ctcaaatgga aaggtcactg gtgttgatgc
     1141 taaccgttac gaattttgga tttatcgtca actgcgaaag cgtttaaagt cgggagaaat
     1201 ctatgtggat gacagtattc aacatcgaaa ttttgctgat gaattagtga atgttagtaa
     1261 tggctcaact atatgcgtta ttttaatcgg tgtatgttca tcaatgagtt ttttccataa
     1321 aatattcgcg caagtagcta aattaccgat taagtctcta caatcggctt catcaatcca
     1381 ttgtggcatg gcttttatta tgattgctgg tgtaacagtt aattctttac ttcgacaata
     1441 aaggccgatt gcacgattca aaatcattgc ttgatcgtaa gaggtacatg tacgatattc
     1501 tgcataactt tctttgaatg agtctgtggt tgctaagctg tcgtgatgat cctcttttgt
     1561 tgtgagagat gcagaatgat attttcttaa agggactcta tagtcttgta aacacaacat
     1621 ttcggccatt tttctaggta aaagccgatt gaaatttagt ttttctttaa tgtaatcagg
     1681 tgtttgtttg aatgctaaac tatggaatgt ttggcactta acattctgag ggaatctctt
     1741 tttggcatcg tctgcaattg ctttattaaa agctaggtat ataccttgtt ggtcgctcaa
     1801 cgtattacca ataagtttta gtgtgctagt ttttccagag ccagcataag cagatatttt
     1861 aaaagttatg ccttctaatg cagaattgat tgctgctatt tgctcttcgg taggtttcat
     1921 ttttgaatat cctagcagat ctttcttaaa aatctattac tcgtttttaa ttggttatga
     1981 cgaataaagt agctttagtc atataggctt gccttgatta tcagcaataa accgtccaca
     2041 gccttcggca tcagcttcct caatatgtac ccaaaaataa cccgatttgt ctttcttttg
     2101 caagcgttta atcagtttag tgcatttagg gcatggctga tttactgatt ctaattttgt
     2161 tggcttccct cgattatctg gaataaaacg ctcgcagtta ttagcatcaa cttcatcaac
     2221 atgcacccag aagaaacctg atttatctct tttctgcaca cgtttaatgg ctttttcaca
     2281 cttagggcat ggttggccaa gagactctag ctttactggc ttgccacgat tatcggcaat
     2341 aaatcgttca cagccttcgg catcagcttc ctcaatatgt acccaaaaat gaccagattt
     2401 gtctttcttc tgtaggcgtt taatcgcttt tgagcactta ggacacgctt gctcaaactc
     2461 tcctaatggc agcaagcctc tgtctagttt tattgcagta atatcttgtt gaataacatc
     2521 atataattgg ctaataacct gattatatgt tgtggcacta ttggctattt catccagttc
     2581 atcttccaaa ttggctgtaa aatcataatc taaaaatttt tgctgtttta aagcatcaat
     2641 aatggctatt cctaattcag ttggaaacaa agattgtttg tttttatctt tgttgtcggg
     2701 tgaaccaact tgtatatagg atttgctaat gatgttttgt acaatcgttg cataagttga
     2761 aggccgccct attcccaaag cttctaattt ttttattaag ctaccttcgg tatatcttgg
     2821 cggtggtgcg gtcattttgc tatcaatttt acttgctatt acgccatgaa tatcgcctac
     2881 ccgtggcact ttttggattt tacctaaaga ctctttatca tctggactat ccaacaccat
     2941 aagttgtcgc caacctttaa ccagctcaac actagcgact gctttaaaca aggccttaat
     3001 ttgttcttca ttaaaggtat agtggttttc gctacacgct tcaatagtcg tgacttcatc
     3061 tatcgctgat ggcatacaag aggccactgc cctttcatgg atgagcgcgt acagttgttg
     3121 tacttgagag tttgagtcaa caacaatgtt attaaaatta gttggtctaa tcgcctcatg
     3181 tgcgccttgg gcgttatctt tggctggcca tttatggggt gtattattgg gagtaaagcc
     3241 tttggatgtt agccattgac ttgcagcatc aaaaccttct gccgacatat ttggattatc
     3301 agtacggtga taggtaatat gtcctgattc aaacaaactt tgtgctatct ccattgtttt
     3361 actagcattt aatttcaatg atgagttagc aac
//
LOCUS       DAOQMB010000249         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15125_length_3368_cov_16.841533, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000249 DAOQMB010000000
VERSION     DAOQMB010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15125_length_3368_cov_16.841533"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLY05_08275"
     CDS             <1..492
                     /locus_tag="PLY05_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNC03337.1"
                     /translation="TEAVWGLGCDPFNEQAVLDLLALKQRPMAKGLILIAANVEQVEP
                     YLQLLTSEQRQTVVDSWPAAKTWVVPVEEQFPIWVRGEHLSVAVRVSAHPPVQALCNA
                     FGGAIISTSANITGKKTAKTTLQIRQQLADGVDYIFSAPLGGLKNPSQIRDARTGQIL
                     RAS"
     gene            504..1418
                     /gene="hemF"
                     /locus_tag="PLY05_08280"
     CDS             504..1418
                     /gene="hemF"
                     /locus_tag="PLY05_08280"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HNC03338.1"
                     /translation="MSANQPDLMAVKAFLLSLQDHICQELLAEDGATAFREDTWQRAE
                     GGGGRSRVLENGAVIEKGGVNFSHVFGSKLPPSATAHRPELAGRSFQAMGVSLVIHPR
                     NPYAPTSHANIRIFIAEKEGEAPVWWVGGGFDLTPYYGFVEDCQAWHQTAKDLCQPFG
                     ADVYATYKKWCDDYFYLKHRQEPRGIGGLFFDDLNQWGFDKCFAFIKAVGKGYIEAYK
                     PILAKRKNTPYGERERNFQLYRRGRYVEFNLVHDRGTIFGLQTGGRTESILMSLPNLV
                     AWQYDYHPEPNSVEARFYQEFLIAKEWV"
     gene            1422..2249
                     /gene="aroE"
                     /locus_tag="PLY05_08285"
     CDS             1422..2249
                     /gene="aroE"
                     /locus_tag="PLY05_08285"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNC03339.1"
                     /translation="MTDRYAVVGNPIGHSRSPRIHRAFAALTQQDLSYEAILSPLEGF
                     RALVNEFFSAPEAKGLNITLPFKEQAYDLSEVKSLRATQAGAVNTLMKGKDGRLYGDN
                     TDGIGLVRDMTENLGWSLANKRILVVGAGGAVRGVLGALLEKNPSQLWVANRTHSKAQ
                     NLANIFRAYGNIAAIRFEDILPQSVDIIVNGTSASLMGDLPPLANNILAEGACAYDMA
                     YGKPQMPFLDWATAQQAQVSDGLGMLIEQAAESFFIWRGVRVQTAELLAEIRKELRV"
     gene            2246..3346
                     /gene="nemA"
                     /locus_tag="PLY05_08290"
     CDS             2246..3346
                     /gene="nemA"
                     /locus_tag="PLY05_08290"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HNC03340.1"
                     /translation="MTMSQLFNPIQVGALSLKNRVIMAPLTRMRSQDETDVPVLPLMA
                     EYYRQRANAGLIISEASQISPQGKGYMGTPGIYSAEQVAAWQQITQAVHQAGGKIAIQ
                     LWHVGRVSHHSLQPNKQLPVSASAIAYVNKTTIRGEDGKPLRVPCDTPRALDLEEIPS
                     LINDYRQATINAREAGFDMIEIHAAHGYLLHQFQSAESNQRQDQYGGSLENRARLTLE
                     VVDACLAVWDAAHVGIRISPLGIFNGLDDQAGLEMGLYLAHEFNKRGLAYLHLSEPDW
                     AGGPAHTDEFRQALREAFTGVIIGAGNYTLDKANVLLDKGFIDAAAFGRPYIANPDLA
                     IRLQKNAELNQLNPDTLYGGGAEGYTDYPALV"
BASE COUNT          935 a          628 c          809 g          996 t
ORIGIN      
        1 accgaagccg tttggggctt ggggtgtgac ccgtttaatg aacaggcggt gcttgattta
       61 ttagccttaa aacagaggcc aatggctaaa ggtttaattt taatcgctgc aaacgtggag
      121 caagttgaac cttatttgca attattaacc tcagaacaac gacaaacagt ggttgattca
      181 tggccagcgg caaaaacatg ggtagtccct gttgaagaac aatttccaat ttgggtgcga
      241 ggcgaacatt tgagtgtggc cgtaagagtg tctgcacatc cacccgttca ggctttatgt
      301 aatgcctttg gtggggctat tatttccacc agtgctaata ttacaggcaa aaaaactgcc
      361 aaaaccacat tacaaattag gcaacagtta gcagatggtg tagattacat cttttccgcg
      421 cctttaggtg gcttaaaaaa tccttcgcaa attcgagatg cccgtactgg ccaaattttg
      481 cgtgcttctt aacagagaaa tctatgtctg ctaatcaacc agatttaatg gctgtcaaag
      541 cctttttatt atccttacaa gatcatattt gccaagagtt attagccgaa gatggtgcaa
      601 ccgcgtttag ggaagataca tggcaacgtg ccgaaggtgg cggtggtcgc tcgcgtgtgt
      661 tagaaaatgg cgcagtgatc gaaaaaggag gagtaaattt ttctcatgtt tttggtagta
      721 aattaccccc ttcagcaaca gcgcatcgtc ccgaattagc aggacgctct tttcaagcga
      781 tgggcgtgtc attagtgatt catccgcgta atccttatgc accaacctcg catgccaata
      841 ttcgtatttt tattgccgaa aaagaaggtg aagcacctgt ttggtgggta ggtggtggtt
      901 ttgacttaac gccttattat ggttttgttg aagattgcca agcatggcat caaaccgcaa
      961 aagacctttg ccagcctttt ggtgcagacg tttacgccac ctataaaaaa tggtgtgatg
     1021 attattttta cctcaagcat cgtcaagaac cacgcgggat tggtggtttg ttttttgatg
     1081 atttaaatca atggggtttt gacaagtgtt ttgcttttat taaagcggtg ggtaaaggct
     1141 atatagaggc gtataagcca attttggcga agcgcaaaaa tacgccctat ggtgagcgtg
     1201 agcgtaattt tcaattatat cgccgtggtc gttatgtcga atttaatttg gtacatgatc
     1261 gtggcacaat ttttggatta caaacaggtg gacgtactga gtctattttg atgtcattgc
     1321 ccaatttagt ggcatggcaa tatgactatc accctgagcc aaattcggtt gaggcgcgtt
     1381 tttatcaaga gtttttaatt gctaaagaat gggtataaat aatgactgat cgttatgctg
     1441 ttgtcggcaa tccaattggc catagtcgct cgccacgtat tcaccgtgca tttgcggcgt
     1501 taacacagca ggatttaagc tatgaggcaa tactgtcgcc tttagaggga tttagagcct
     1561 tagtaaacga gtttttttct gcaccagaag ccaaaggctt aaatattacc ttgccattta
     1621 aagaacaagc ttatgattta agtgaagtaa agtctttaag agcaacacaa gcaggggcag
     1681 taaatacctt aatgaaaggt aaagatggcc gtttgtatgg cgataatacc gatggcattg
     1741 gccttgtgcg tgatatgacc gaaaacttag ggtggagctt agccaataaa cggattttag
     1801 tcgtcggtgc aggtggtgca gtacgtggtg ttttaggcgc attattagaa aaaaatccta
     1861 gtcaattatg ggtagctaat cgtacccata gcaaagcgca gaacttggct aatatttttc
     1921 gtgcttatgg taatattgct gcgattcgtt ttgaggatat tttgccccaa agcgtggata
     1981 ttatcgttaa tggtacatca gcctcattaa tgggggattt accaccatta gccaataata
     2041 ttttggctga aggggcttgt gcttatgata tggcctatgg caaaccacaa atgccctttt
     2101 tggattgggc aacagcacaa caagcacaag tgagcgatgg tttagggatg ttgattgaac
     2161 aagccgccga aagctttttt atttggcgcg gtgtgcgtgt gcaaacggct gaattattag
     2221 cagaaattag aaaagaatta agagtataac catgagtcaa ttatttaatc ctattcaagt
     2281 gggtgcattg agtttaaaga atcgtgtgat tatggcgcct ctaacacgga tgcgtagcca
     2341 agatgagact gatgtgcctg tcttgccgtt aatggcagag tattatcgtc aacgagctaa
     2401 tgcgggtttg attatcagtg aagcttcgca aatttcgccc caaggcaagg gctatatggg
     2461 tacaccaggg atttatagtg ctgagcaagt ggctgcatgg caacaaatta ctcaggcggt
     2521 acaccaagca gggggtaaga ttgcgattca attatggcat gttgggcgtg tgtcacatca
     2581 ctcattacag cccaacaaac aattgcctgt ttcggcctct gcgattgctt atgtcaacaa
     2641 aaccacgatt cgtggcgaag atggcaaacc tttacgcgtg ccttgtgaca cgcctcgtgc
     2701 attagattta gaagaaatcc ccagtttgat taatgattat cgtcaagcca ccatcaatgc
     2761 gcgtgaagca ggctttgata tgatagaaat tcatgcggca catggttatt tgttgcatca
     2821 atttcaatca gccgaaagca atcagcgtca agatcaatat ggtggcagct tagaaaatcg
     2881 tgcgcgttta accttagaag tggttgatgc ctgtttggcg gtgtgggatg cagcccatgt
     2941 tggtattcgt atttcaccat taggcatttt taatggttta gacgatcaag caggtttaga
     3001 aatgggcttg tatttagctc atgaattcaa caaacgtggt ttagcttatt tgcatttatc
     3061 tgagccagat tgggcaggtg gccctgcaca cacagatgaa tttagacaag ccttacgtga
     3121 agcttttaca ggtgtcatta ttggtgcagg aaattacacc ttagataaag ccaatgtctt
     3181 gttagataaa ggatttattg atgccgcagc ctttggtcgt ccttatattg ctaatccaga
     3241 tttagccatt cgtttgcaaa aaaatgctga gttaaatcaa ttaaatcctg acacattata
     3301 tgggggtggt gcagaaggtt ataccgacta tcctgcattg gtttaataaa tacgcaagct
     3361 ctttttgg
//
LOCUS       DAOQMB010000250         3368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15126_length_3368_cov_16.376094, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000250 DAOQMB010000000
VERSION     DAOQMB010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15126_length_3368_cov_16.376094"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="PLY05_08295"
     CDS             complement(<1..221)
                     /locus_tag="PLY05_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002067913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HNC03341.1"
                     /translation="MAGRIAQNFIDEVLRRTDIVDLIGSYVRLKKAGKNYSACCPFHH
                     EKTPSFSVNRDKQFYHCFGCGASGNALSFV"
     gene            complement(225..617)
                     /locus_tag="PLY05_08300"
     CDS             complement(225..617)
                     /locus_tag="PLY05_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03342.1"
                     /translation="MRLLLDTHIFLWFISGHARLSPQLVEILRNPQHQIFVSVISIWE
                     CTIKQQLGKLPLPAPAPIYLPLQREKHQFHSLDISEFSLKHLYNLPSIHRDPFDRMLI
                     SQALAHQLTLVSEDNAIAAYSVPLLKLD"
     gene            complement(617..856)
                     /locus_tag="PLY05_08305"
     CDS             complement(617..856)
                     /locus_tag="PLY05_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03343.1"
                     /translation="MVTVSVQEIQSNFLHYLSYLQSGESIIIMQENHAIAELSPAKNS
                     SIVQRPFALCQQDFVVPDNFDEPLPNDILDAFEGK"
     gene            complement(923..>1063)
                     /locus_tag="PLY05_08310"
     CDS             complement(923..>1063)
                     /locus_tag="PLY05_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007115521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNC03344.1"
                     /translation="LINAAVAEVGATSARDMGKVMNILRPQIAGRADGAEVSKLVKAK
                     LG"
     assembly_gap    1064..1163
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1338..1553)
                     /gene="rpsU"
                     /locus_tag="PLY05_08315"
     CDS             complement(1338..1553)
                     /gene="rpsU"
                     /locus_tag="PLY05_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HNC03345.1"
                     /translation="MPLVKVKEGEPFDVALRRFKRSCEKAGVLADVRKRDFYEKPTQE
                     RKRKKAAAVKRYAKKLARESVKTTRMY"
     gene            1765..2781
                     /gene="tsaD"
                     /locus_tag="PLY05_08320"
     CDS             1765..2781
                     /gene="tsaD"
                     /locus_tag="PLY05_08320"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004900574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNC03346.1"
                     /translation="MRVLGIETSCDETGVAVYDSEQGLLAHRLYSQIQLHADYGGVVP
                     ELASRDHVRKLIPLIKEVLAAANSQATDINGIAYTSGPGLAGALMVGALTGRSLAYAW
                     RVPAIGVHHMEGHLLAPLLEENKPEFPFIALLVSGGHTQLLKVEGIGQYELLGESVDD
                     AAGEAFDKVAKMLHLDYPGGPNVARLALQGNSEAFNFPRPMLDRAGLDFSFSGLKTAV
                     LNANNQTEDYPQKKADIAASFEAAIVDSLVKKCERALKQTGLKRLVMAGGVSANLRLR
                     AQLQDLATRKRWQVFYPSAAFCTDNGAMIAYAGCQRLVAGQQENLSVTVKSRWPMVEL
                     PAVN"
     gene            2795..3355
                     /locus_tag="PLY05_08325"
     CDS             2795..3355
                     /locus_tag="PLY05_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03347.1"
                     /translation="MLRYWLMLLLLVQSFCLQAETLTINEKSFKCLSQMTPVRGFFVD
                     SLTGNLADTLAVAQSPTGGVYPVGSVVQLVPTEVMVKREQGFSPVTKDWEFFELDVDK
                     QGSKIRKRGFADVNNRFKKNCFACHAAAKPEWDMICEDSHGCEKLPIPEHVIVALQKT
                     DPRCPTNDATAMQRFSAWLVQKFSPN"
BASE COUNT          974 a          621 c          705 g          968 t
ORIGIN      
        1 acaaaactta aggcattacc tgacgcgcca caaccaaaac agtgataaaa ctgtttgtca
       61 cgattaacac taaacgatgg ggttttttcg tggtgaaaag gacaacaagc cgaatagttt
      121 ttgcctgctt tttttaggcg cacataacta ccgattaaat cgacaatatc ggtacgtctt
      181 aacacttcat caataaagtt ttgtgcaata cggccagcca tagtttaatc aagttttaac
      241 agaggaacgg aataggcggc aatcgcatta tcttcactca ctaaggtcag ttgatgggct
      301 aatgcttgac tgattaacat tctatcaaat gggtctcgat ggatagaggg aagattatat
      361 aagtgtttta aactaaactc gctaatgtcg aggctatgaa attgatgttt ttctctttgc
      421 agtggtagat aaataggcgc aggtgcgggc aaaggaagtt tacctaattg ttgtttaatg
      481 gtgcattccc aaattgaaat cacactgaca aaaatttgat gttgagggtt acgaagtatt
      541 tctacaagtt gaggagagag acgtgcgtga ccactgataa accataaaaa gatgtgagta
      601 tctaataata agcgcattat ttaccttcaa aggcatctaa aatgtcatta ggtaaaggct
      661 catcaaaatt atcaggtaca acaaagtctt gttggcataa agcaaatggg cgttgaacaa
      721 tagagctatt ttttgcaggg cttaactcag caattgcatg attttcttgc atgataataa
      781 tgctttcgcc agattgtaga taagacaaat aatgtaaaaa attactttgt atttcttgca
      841 cagatacagt gaccattaag cacctcaata cgttaatatc ctttgtttaa ggatattaac
      901 agtatagcca acaaatacca aatcaaccca atttagcttt taccaattta gacacttcag
      961 caccatcggc acgacctgca atttgtggac gtaaaatatt catcacttta cccatatcac
     1021 gcgcactcgt tgcacccact tcggcaacag cagcattaat taannnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnngcttctt tcatggcatc ggttaattga gcttttaaag
     1201 tcatgttaat cttccttatg tttttttgac catcaataaa aaataaacgc cttatgagtc
     1261 ataaggcgtt tatttaacaa atcaccgttt tacgatgatt ttactatcta gcccccaaat
     1321 agggctaaga taaataatta gtacatacgg gtggttttaa ccgactcgcg tgctaatttt
     1381 ttagcataac gtttaacagc agcagctttt ttacgcttgc gctcttgagt tggcttctcg
     1441 tagaagtcac gtttacgcac atcagctaaa acacctgctt tttcgcaaga acgtttaaaa
     1501 cgacgtaatg ctacgtcaaa tggttcgcct tcttttactt tcactagggg catgaatcat
     1561 caccttaatc taaaaacgaa aaccacccta gtcgggcaga aaaccaagct acttgtattt
     1621 agcacaagct agctatgaat acaaagaggc ggcaatttta cgcaaagata gcgttaacag
     1681 caagcagcgc gtacaataaa ctctagttaa ttgtcaataa attcgctctt ttttgcaaaa
     1741 aaaggctaat taaaggggta aattatgcgt gtcttgggga tagaaacctc ttgtgatgaa
     1801 acaggtgttg ccgtttatga tagcgaacaa ggcttattag ctcaccgttt atacagtcaa
     1861 attcagcttc atgccgatta tggtggtgtt gtgccagagt tggcctcgcg tgaccatgtg
     1921 cgtaaattga ttcctttaat taaagaagtg ttggccgctg caaatagcca agccacagac
     1981 attaatggca ttgcctacac ttctgggccg ggtttggctg gtgcattaat ggtcggggca
     2041 ttaacaggcc gtagtttggc ttatgcgtgg cgtgtacctg cgattggtgt gcatcacatg
     2101 gaagggcatt tattagcacc tttattagaa gaaaataagc cagagtttcc ctttattgcc
     2161 ttattggtat ctggtggcca tacgcaacta ttaaaggttg aaggtattgg ccaatatgaa
     2221 ttattgggtg aatcggttga tgatgccgca ggtgaagcct ttgataaagt cgccaaaatg
     2281 ctccatcttg attacccagg tgggccaaat gtggccagac tcgccttaca aggcaatagt
     2341 gaagctttta attttccgcg tcctatgctt gaccgagcag gtttagattt ttcatttagt
     2401 ggtttaaaaa cggctgtgtt aaatgcgaat aatcaaaccg aagattatcc acaaaaaaag
     2461 gcggatattg ctgccagttt tgaagcggcg attgttgata gccttgttaa aaaatgtgaa
     2521 cgagccttaa aacaaacagg gttaaagcgt ttggtgatgg ctggcggcgt gagtgctaat
     2581 ctgcgtttac gcgcccagtt acaagacttg gcaacacgta aacgttggca agtattttac
     2641 ccctcagctg ctttttgtac cgataatggt gcaatgattg cttatgcagg ttgtcagcgt
     2701 ttggtggcag ggcagcaaga aaacttatcg gtgacagtaa aatcacgttg gccaatggtt
     2761 gaattgccag cagttaatta agaggggata gaaaatgcta cgttattggt taatgttatt
     2821 gctattggtg caaagttttt gtttacaggc ggagacttta accattaacg aaaaatcctt
     2881 taaatgcctc agccaaatga cacccgtacg tggttttttt gttgatagct taacaggtaa
     2941 tttggccgac actttagcag tagctcaatc acccacaggc ggagtttatc ctgtgggttc
     3001 tgtggtgcaa ctcgtaccca ccgaagtgat ggtcaaacga gaacaaggtt ttagtcctgt
     3061 gactaaggat tgggagtttt ttgagttgga tgttgataaa caaggctcaa aaattcgcaa
     3121 acgtggtttt gctgatgtga ataaccgctt taaaaagaac tgtttcgcct gtcatgccgc
     3181 agcaaaacca gaatgggata tgatttgtga agactcacat ggttgtgaaa agctgcctat
     3241 tcctgagcat gtaatcgttg ccttacaaaa aaccgatcca cgttgcccta ctaatgacgc
     3301 aacggctatg cagcgtttta gtgcgtggtt ggtacaaaaa ttctcgccaa attgattgca
     3361 tctttagt
//
LOCUS       DAOQMB010000251         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15233_length_3352_cov_16.628147, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000251 DAOQMB010000000
VERSION     DAOQMB010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15233_length_3352_cov_16.628147"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1573
                     /locus_tag="PLY05_08330"
     CDS             95..1573
                     /locus_tag="PLY05_08330"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNC03348.1"
                     /translation="MKVSLNIQMGQHLTMTPQLQQAIRLLQLSTIELQQEIQNALDSN
                     PLLELHEDYDNNATTKAETNTDDNRQDDYETELSASTVDINDVQNIPEDLPVDSAWDD
                     IFTGNSANTNTPDEDDEGFEARSTVAETLYDHLLWQVNLTPFTITDRTIAVCIIDSLD
                     ENGYLHNDLEDILEAAQNILPNEDIELDEIVAVLHLIQQFEPSGVAARNLQECLKIQL
                     KQLPEDTAHRENALLIVNEHLDLLAAQDQQGLLKRTKFSESQLRAAVGLIQTLNPYPG
                     GKIGQDENSYIIPDVVVSKQKNRWRVELNPDTAPKLRINTSYASLIRRADSSADNTYL
                     KNHLQEARWFLKSLQSRHETLLKVATCIVEHQRAFLEQGPEAMKALVLRDIAEEVELH
                     ESTVSRVTSQKYMLTPRGLFELKYFFSSHVGTSSGGECSSTAIRAMIKKLVSQEDPRK
                     PLSDNKIADLLQEQGIEVARRTIAKYRESLNIPSSSDRKRLL"
     gene            1880..2170
                     /gene="raiA"
                     /locus_tag="PLY05_08335"
     CDS             1880..2170
                     /gene="raiA"
                     /locus_tag="PLY05_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNC03349.1"
                     /translation="MQMIISGHHVEVTPALKEYVQAKFAKVERHFDHITSMQVILSIE
                     KLAQKAEATIRLSGAELFANAESIDMYAAIDLLSDKLDRQIVRHKEKLKSHH"
     gene            2353..3210
                     /gene="rapZ"
                     /locus_tag="PLY05_08340"
     CDS             2353..3210
                     /gene="rapZ"
                     /locus_tag="PLY05_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HNC03350.1"
                     /translation="MNNLLIVSGRSGSGKSSALNILEDLGYYCIDNLPLSLLPSLIVH
                     LKSTSSIQKVGVGVDVRSLPDDFNNFHQVISDIEKLGIQTNTLFLDARDDILIARFSS
                     TRRRHPLSDQNTSLKEAIEKETLLLDPIASKASIYLDTSSLNVHELQHLLAVRLSGDN
                     QVTLLLESFGYKHGVPLDADFVFDVRCLPNPHWQSSLRELTGKQQEIKDFLNGHPEVE
                     ALYYDISQFLIKWLPRLSADHRHYLTVAIGCTGGQHRSVYMVERLYESLKTILPHIQT
                     LHRELVRRK"
BASE COUNT         1120 a          644 c          578 g         1010 t
ORIGIN      
        1 gaattaatgg actgtttttt gcatggcgat tttgagagag acgttatata ctgatattta
       61 ttctcagtct agttagtaaa gtgtagattt gccaatgaaa gtctctctca atattcaaat
      121 gggtcaacat ctgaccatga ccccacaatt gcaacaagcg attcgtctct tgcaattatc
      181 gactattgaa ttacaacaag agatccaaaa cgcattagac tctaaccctt tattagaatt
      241 acacgaagat tatgataata atgctacaac taaagcagaa actaataccg atgataaccg
      301 tcaagatgat tacgaaaccg aattatcagc aagcactgtt gatattaatg atgtacaaaa
      361 tattcccgaa gacttgcctg tagatagtgc atgggatgac atttttacag gtaatagtgc
      421 caataccaac acccccgacg aagatgatga aggttttgaa gctcgctcta cagttgccga
      481 aactttatac gatcatttat tatggcaagt taatctgaca ccattcacta tcactgaccg
      541 aaccattgcg gtttgtatta tcgactcttt agatgaaaat ggctatcttc ataacgattt
      601 agaagatatt ttagaagctg cacaaaatat tttacctaat gaagatatag agttagatga
      661 aattgttgca gtattacatt taattcaaca atttgaacct tcaggcgttg ctgcacgcaa
      721 tttacaagaa tgtttaaaaa ttcaactcaa acaactgccc gaagatactg cccatcgaga
      781 aaatgcttta ttgattgtta atgaacattt agatttacta gcagcccaag accaacaagg
      841 cttattaaaa cgtaccaaat ttagcgaaag tcagttacgc gctgctgtag gattaattca
      901 aacacttaat ccctaccctg gtggcaaaat tggtcaagat gaaaatagct atatcattcc
      961 tgatgtggtt gttagcaaac aaaaaaatcg ctggcgtgtt gagctaaatc cagacacagc
     1021 cccaaaattg cgaatcaata ccagttatgc aagcctcatt cgtcgagcag atagtagcgc
     1081 ggacaatact tatctaaaaa atcacttaca agaagcacgc tggtttttaa aaagtttaca
     1141 aagccgacac gaaaccctgc tcaaagttgc cacctgtatt gtggaacacc aacgtgcttt
     1201 tttagaacaa ggcccagaag ccatgaaagc cttagtttta cgtgacattg ctgaagaagt
     1261 tgaattacac gaatcaactg tctctcgtgt cactagtcaa aaatatatgc tcacacctcg
     1321 cggcttattt gaactcaaat atttcttctc tagtcatgta ggcacaagct ctggtggaga
     1381 atgttcttca accgcaattc gagcaatgat taaaaaatta gtcagccaag aagatcctcg
     1441 caaacctttg tctgataaca agatcgctga tctattacaa gaacaaggta ttgaagtcgc
     1501 acgtcgtacc attgctaaat atcgcgaatc gctcaatatt ccatcatcta gtgatagaaa
     1561 acgcttgctt taactttttt tttgtggcaa aacagaatca cgagataaat attttaagtt
     1621 tcatcttact gtaacacttt gttactaaag gacttaaaaa attctcgttg gcttttagcg
     1681 aagagctaac ttcgttaggc cgaacgacct gaaccatcaa ttaacatccg ctaaaaactt
     1741 atagcaaagt gttaattaga ctatgatggc cgcccgacca aacaggctct tcgcaatggt
     1801 cttatgtaat acttatttga ttaataataa gttttcataa gtctttgagt ttgtcacaaa
     1861 aacacgagga acggttatta tgcaaatgat tatcagtggt catcacgtcg aagttactcc
     1921 cgccttaaaa gaatacgtgc aagccaaatt cgccaaagta gaacgccact ttgaccatat
     1981 taccagcatg caagttatcc tcagcattga gaagttagcc caaaaagcag aagcaacgat
     2041 tcgcttatct ggtgctgaat tattcgctaa tgcagaatct atagatatgt atgctgctat
     2101 tgacttactc agtgacaaat tagaccgcca aattgtgcgc cataaagaaa agttaaaaag
     2161 tcatcactaa taaaaaaagc ccaatcaatg attgggcttt tttatcattc ttcacaaatc
     2221 agataaaaat tggcttcata ttatattaaa agatagacag ttgcataaaa tcactatatt
     2281 tttagcattt ctaacataac gatttcgtta aaatattcat gtaattcaat tattacattg
     2341 caaaaaatta ttatgaacaa tttattaatt gtaagtggtc gctctgggtc tggcaaaagt
     2401 tcagctctaa atatattaga agacttaggc tattactgca tagacaactt acctcttagc
     2461 ctactcccct cactcatcgt ccatcttaaa agcacatcat ctattcaaaa agttggtgtg
     2521 ggtgttgatg tacgcagttt acctgatgat tttaataatt ttcatcaagt catttctgat
     2581 attgaaaaac taggcattca aactaataca ctttttttgg atgctcgtga tgatatttta
     2641 atcgcgcgtt ttagttcaac acgtcgtcgt catccattaa gtgaccaaaa cacttctctt
     2701 aaagaggcca tagaaaaaga gacgttgtta ttagatccaa tagcatctaa agcaagtatt
     2761 tatttagata ctagtagtct aaatgtgcat gaactacagc atttattagc tgttcgatta
     2821 tcaggtgata accaagtgac cttgttatta gagtcatttg gctacaaaca tggtgtacca
     2881 ttagatgccg attttgtctt tgatgtacgc tgtttaccta atccacattg gcaaagctcc
     2941 ctacgtgaat taacaggtaa acaacaagaa attaaagatt ttttaaatgg tcaccccgaa
     3001 gttgaagctt tatactatga tataagtcag tttttaatca aatggttacc tagactaagt
     3061 gctgaccatc gccattattt aacagttgcg attggttgta caggcggtca acatcgctcg
     3121 gtatatatgg tcgaacgatt atatgagtct ttaaaaacca ttttacctca cattcaaact
     3181 ttacatagag aattggttcg tcgtaaatga ttcaagcgga tatagaaatt atcaataaac
     3241 tcggattaca tgcgcgtgct acagccaagc tgattgcact gacctcgact tttcgttgta
     3301 atatcaaaat tactaaaggg actcgtaccg tagatggtaa aaatatgatg gg
//
LOCUS       DAOQMB010000252         3351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15241_length_3351_cov_13.968143, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000252 DAOQMB010000000
VERSION     DAOQMB010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15241_length_3351_cov_13.968143"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="PLY05_08345"
     CDS             <1..2227
                     /locus_tag="PLY05_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03351.1"
                     /translation="GLGISMTKGGDIITLGKDLQHASQKLRDSLPAGIELQQVQDQPK
                     SVNRSVQEFVKTLAEALVIVLVVSFLALGLHTKPLRVDMRPGLVVGLSIPLVLAITFL
                     IMDRWGIDLHKISLGSLIIALGLLVDDAIIIVEMMVRKLEEGYDRLSASIYAYQTTAM
                     PMLTGTLITAAGFLPIGLANSAVGEYTFAIFAVTTTALIVSWFVSVYFVPFLGYRLLK
                     THHAGSAEHHEVFDSPFYNAVRNTVAWCVNHRWITLLATLAALGAGIMGMKLVEKQFF
                     PDSNRPEMVIDLWLPEGSSLTNTESIVKRVEQRLLAIPEAGSVTSFVGQGAPRFYLSL
                     DQIFPQNNVAQLVIMPPSLEAREKIRHQLPVMLQEEFPEIRGRARLLPNGPPVPYPVM
                     FRVIGDNPDKLRAIADEVKTIVRADSAMRGVNDNWSEEVKSLHLDIDQQRARALAVTS
                     SDIATASETILSGQTIGTYREENDQIPIILRQPKEERDSMTALTGTYLPTGSGQSIPL
                     AQIARPHLQWEYGVVWRQNRQFAITVQGDVADGIQPATVALRINEKLEALRAKLDAGY
                     HIEIAGTVAESQKGTSSIQANVPLMLFIIFTLLMLQLRSFPRALLVFLTGPLGVIGAA
                     LMMLALNRPMGFVATLGIIALNGMIIRNSVILIDQIEQDIANGVDRWQAVVEAAVRRF
                     RPIILTAAAAVLAMIPLMHSTFWGPMAVAIMGGLIVATALTLLSLPAMYAIFFSVKKA
                     V"
     gene            2314..2745
                     /locus_tag="PLY05_08350"
     CDS             2314..2745
                     /locus_tag="PLY05_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNC03352.1"
                     /translation="MSTAYKTDIIAWANEQAAFIRAGRFDLLDLENIAEEIEDVGKSE
                     QRELENRMSILLAHLLKWQFQEARRGSSWQLTIKEQRKRIIMRLKETPSLKKSLNDDN
                     WQEAVWSDAVAKAAEETGLDNFPEVCPWTFEQILNQDFLPE"
     gene            complement(2871..>3351)
                     /locus_tag="PLY05_08355"
     CDS             complement(2871..>3351)
                     /locus_tag="PLY05_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03353.1"
                     /translation="ELIERFEDKWDWHNFSWNEALPWSLELIERFEDKWDWQSLSGNE
                     ALPWSLELIERFEDKWGRHNFSWNKALPWSLELIERFEDKWDWQSLSGNKALPWSLEL
                     IERFEDKWDYFWLGLNQKAIECLPKLSLEDIDEVMSYHFPLPQISKQSNDFDDDLPF"
BASE COUNT          923 a          716 c          720 g          992 t
ORIGIN      
        1 tggtttaggc atttccatga ccaaaggcgg tgacatcatc accttgggta aagacttaca
       61 acatgccagt caaaaactac gcgacagctt acctgctggt attgaattgc aacaagtaca
      121 agaccaacct aaatcggtta atcgctcagt gcaagagttt gttaaaacct tagccgaagc
      181 cttagtaatt gttttagtgg ttagcttttt ggcgttaggc ttacacacca aaccgttaag
      241 agtcgatatg cggccgggtt tagtggtcgg tttatcgatt cctttggtat tagcaattac
      301 ctttttaatc atggacagat ggggcattga tttacacaaa atctcgctcg gctcgctgat
      361 tattgccctt ggcttgttgg tggatgatgc gattattatt gtcgaaatga tggtgcgtaa
      421 actcgaagaa ggttatgacc gtttaagtgc gagtatttat gcttatcaaa ccactgctat
      481 gcccatgctc acaggcacat taattacagc cgcaggcttt ttaccgattg gtttagccaa
      541 ttctgcggtg ggtgaataca cctttgccat ttttgcggta acgacgacag ccttaattgt
      601 ctcgtggttt gtctcggtct attttgtgcc atttttaggg tatcgtttat taaaaactca
      661 tcatgcaggt tcagccgaac atcacgaagt ctttgattcg cctttctata atgcggtgcg
      721 taacaccgtg gcatggtgtg tgaatcaccg ttggattacc ttactcgcta ccctcgccgc
      781 gcttggtgca ggtattatgg gcatgaaatt ggttgaaaag cagtttttcc ccgactccaa
      841 ccgtccagaa atggtcattg atttatggtt gcccgaaggt tcttcgttaa ccaataccga
      901 aagtatcgtc aaacgagttg aacaacgttt attagccatt cccgaagcag gttcggtgac
      961 ttcgtttgtt ggtcaaggcg caccgcgttt ttacttatcg ttagaccaaa tctttccgca
     1021 aaataacgtc gcccaattgg tgattatgcc gccctcttta gaagcgcgtg aaaaaattcg
     1081 tcatcaatta cctgtcatgt tacaagagga atttccagaa attcgtggcc gtgcgcgatt
     1141 attacccaat ggcccacctg tgccctatcc tgtgatgttt agagtgattg gtgataatcc
     1201 tgataaattg cgtgcgattg ccgatgaagt taaaaccatt gttcgtgcag actctgcgat
     1261 gcgtggtgtt aacgataact ggtctgaaga agtcaaatct ttacacttag atattgacca
     1321 acaacgggcg cgtgctttgg cagtcactag ctctgatatt gccaccgcta gcgaaaccat
     1381 tttatcagga caaactattg gcacataccg cgaagaaaac gaccaaatcc caattatttt
     1441 gcgtcagcct aaagaagaac gagacagcat gactgcctta acaggtactt atttgcccac
     1501 aggtagtggc cagtctattc ctttagcaca aattgcgcgt cctcatttgc aatgggaata
     1561 tggtgtggtg tggcggcaaa atcgtcagtt tgccattacc gtacaagggg atgtggctga
     1621 tggtattcaa cctgccaccg ttgccttacg cattaatgaa aaacttgaag ccttacgcgc
     1681 taagttggat gctggctacc atattgaaat tgcagggaca gtcgctgagt cacaaaaagg
     1741 cacatcgtcc attcaagcca atgttccttt gatgttattt attatcttta ccttgttgat
     1801 gttgcaatta cgtagcttcc cacgcgcgtt attggtgttt ttaacaggac ctttaggagt
     1861 gattggtgcc gcgttaatga tgttagcact caaccgtccc atgggttttg tggcgacact
     1921 cggtattatt gcccttaacg gtatgattat tcgtaactca gtgattttga ttgaccaaat
     1981 tgagcaagat attgccaatg gtgttgatag atggcaagcc gtggttgaag cggcggtacg
     2041 tcgttttaga ccgattattt taacggctgc ggctgcggtt ttagcgatga ttccgttaat
     2101 gcacagcacc ttctgggggc caatggctgt ggccattatg ggtgggttga ttgtggcaac
     2161 ggctttgact ttgctcagtt tacctgcgat gtatgccatt ttctttagcg tgaaaaaagc
     2221 agtgtaaaat gatgaaatta gcccgtatgt gccaaaatac gggttatatt attaagtaaa
     2281 cacaggctaa atacacaagt tgagggttaa acgatgagta ctgcctacaa aactgacatt
     2341 attgcatggg caaacgaaca agctgcgttt attcgtgcgg gtcgctttga cctactagat
     2401 ttggaaaaca ttgccgagga gatagaagac gtgggcaaaa gtgaacaacg cgaacttgaa
     2461 aaccgtatgt ctatactatt ggcgcattta ttaaaatggc aatttcaaga ggcaagacgc
     2521 ggttcaagtt ggcaactgac gattaaagaa caacgtaaac gtattattat gcgacttaaa
     2581 gaaacgccta gcttaaaaaa atcgctaaac gatgacaatt ggcaagaagc ggtatggtct
     2641 gatgcagttg caaaagctgc cgaagaaaca ggtcttgata actttccaga agtttgccca
     2701 tggacatttg agcaaatact taatcaagat tttttgcctg agtagtacag cctagtagcc
     2761 cgtgtgaagt gcaacgcaac acgggattta tggccaaatc aaagcaataa gtatctgttt
     2821 tatatatatt taatcccgta tttcgctacg ctgcatacag gctactcttg ctaaaatggc
     2881 aaatcatcat caaaatcatt tgattgcttt gatatttggg gtaacggaaa gtgataactc
     2941 atcacttcat caatatcctc tagtgataat tttggtaaac actctatagc cttttgattg
     3001 agacctaacc aaaagtaatc ccatttatct tcaaaccgtt caataagctc taatgaccac
     3061 ggcaaggctt tattccctga caaactctgc caatcccact tatcttcaaa tctttcaatt
     3121 aactctaatg accacggcaa ggctttattc catgaaaaat tatgcctacc ccacttatct
     3181 tcaaatcgtt caattaactc taatgaccac ggcaaggctt cattccctga caaactctgc
     3241 caatcccatt tatcttcaaa ccgttcaata agctctaatg accacggcaa ggcttcattc
     3301 catgaaaaat tatgccaatc ccacttgtct tcaaatcttt caatcaattc t
//
LOCUS       DAOQMB010000253         3315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15517_length_3315_cov_13.618712, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000253 DAOQMB010000000
VERSION     DAOQMB010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15517_length_3315_cov_13.618712"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..984)
                     /locus_tag="PLY05_08360"
     CDS             complement(112..984)
                     /locus_tag="PLY05_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03354.1"
                     /translation="MKFFRHLVLSSMLISSAQTYAANWVDDLLNLTGTSLIFEQNIVQ
                     QALQDNQTIPDLNQLLENSHFQAWAGARLWIVYSANGQLQSYPLKEAINNEANQRGLL
                     LSNKLSANDITLLNNAITSYKTNSQPLKTLLQQQQYDGLVLINQKDNIFTWKILTPNQ
                     QFLGAISQDGLIYLPHIWAENLGMAWQWPDLNNDFLVRIDNIKLFEQFIQSEQILKTH
                     CSQVRLLSIDGYQANFACQTNLSYSQLHDKLRLIPLFSLKQNAVAASSLPTPVLMGRQ
                     LSQRFLNYQWLNNF"
     gene            1140..>2143
                     /gene="purM"
                     /locus_tag="PLY05_08365"
     CDS             1140..>2143
                     /gene="purM"
                     /locus_tag="PLY05_08365"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016137333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNC03355.1"
                     /translation="MSNTQTSLSYKDAGVDIDAGDHLVERIKGVAKKTTRPEVLGGLG
                     GFGALCEIPAGYKQPVLVSGTDGVGTKLKLALQLKRHESIGVDLVAMCVNDLVVCGAE
                     PLFFLDYYATGHLSVDTAATVITGIGEGCLQAGCALVGGETAEMPGMYEGEDYDLAGF
                     CVGVVEKSQIIDGSKVKAGDVLIGIASSGVHSNGYSLVRKVIEVAQADLSQPLDGKTL
                     ADVVMAPTRIYVKSMLSLIKAVPVHAIAHITGGGLPGNLPRVLPEGADAVIDEKSWQW
                     PAIFQWLQQQGNVERFEMYRTFNCGVGMVVVVPADKADEAIAHLQANGEQAWRLGQIQ
                     P"
     assembly_gap    2144..2243
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2244..2585
                     /locus_tag="PLY05_08370"
     CDS             <2244..2585
                     /locus_tag="PLY05_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004799523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNC03356.1"
                     /translation="FEGKLINIHPSLLPLYKGLHTHQRVLATGDKLHGCSVHFVNAEL
                     DGGAVIAQAVLTVAANEAEQSLIQRVHKLEHIIYPLVLNWLSYGRLSYQQGLPVLDGQ
                     TLSTPMKLLFD"
     gene            2631..3314
                     /locus_tag="PLY05_08375"
     CDS             2631..3314
                     /locus_tag="PLY05_08375"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HNC03357.1"
                     /translation="MRFLLLITSLSLSVKTFAFDLQPYTATYQFNLNQQITGTATRTL
                     SKQENNHYRYQFNATAAIANANEVSEFIFDKQQVQSLSYQNSKQVLFKNRVDQATFDW
                     DKQQLTTLRKGQNETFPLTDKTVLDPLNLEIQIRQDLSQQTKPQLKEYLLGDAKGFQP
                     LKFIVDGVEKIKIPYGEIEALKVTRIHQSSDRQTQFWLAKSLDYLPVKVVQMDDGAIY
                     TIELQALQK"
BASE COUNT          978 a          534 c          674 g         1029 t
ORIGIN      
        1 gcaaaaaaag gcataagaat agattgtagg gcatagagca gccaaattaa taccgcaaaa
       61 actacccaaa taggccaacg attaattata ttattcatca tttatattat tctaaaaatt
      121 gtttaaccat tgatagttta aaaagcgttg gctgagttgt ctgcccatta aaacaggtgt
      181 aggcaaactg cttgccgcta ctgcattttg tttgaggcta aatagaggaa tcagccgaag
      241 tttatcatgc aattggctat aacttagatt agtttgacaa gcaaaattgg cttgatagcc
      301 gtcgatgctt aataaacgta cttgcgaaca atgtgttttt aaaatttgtt cactttgaat
      361 aaattgctca aatagtttga tgttatctat tctaactaaa aaatcattat ttaaatctgg
      421 ccattgccat gccatgccta aattttctgc ccaaatgtga ggtagataga ttaatccatc
      481 ttggctaatc gcgcctaaaa actgttgatt aggtgttaaa attttccatg taaatatatt
      541 gtctttttga ttaattaata ccaagccatc atattgctgt tgttgtaata aagtttttaa
      601 tggctgagag ttggttttat aactcgtaat cgcattattg agtagcgtaa tgtcatttgc
      661 ggaaagttta ttgcttagaa gtaatccacg ttgattggct tcattgttaa tggcttcttt
      721 aagtggataa ctttgtaatt ggccatttgc agaatataca atccacaagc gagcaccagc
      781 ccatgcttga aaatgactat tttctagaag ttgatttaag tcagggatag tctgattgtc
      841 ttgtaaagct tgttgtacga tgttttgttc aaaaatcaaa ctcgtacccg tcagattaag
      901 taaatcatct acccagttag ctgcataagt ttgagcggag ctgataagca tagaacttag
      961 tactaaatga cgaaaaaact tcatttatga catcctgata agacaaactt gcgtaccata
     1021 cgcaacttga cgtggaattt ctaggcttta ttgctgttaa tagtggagca aagcacgata
     1081 aaacgctaaa atgcgccccc aaatttcctt tttatttatt tttgtggaaa gaaatcacca
     1141 tgagcaatac acaaacttcg ttaagctaca aagatgcagg cgttgatatt gatgcaggcg
     1201 atcatttagt tgaacgtatt aaaggtgtcg ctaaaaaaac gacacgtcct gaagtattgg
     1261 gtggtttagg tggttttggt gctttatgtg aaattccagc aggctacaaa caacctgttt
     1321 tagtctctgg cacagatggt gtgggtacta aattaaaatt agctttacag ttaaagcgtc
     1381 acgaaagcat tggtgttgat ttagtggcga tgtgtgtcaa tgacttagtg gtgtgtggtg
     1441 cagagccatt attcttttta gattattatg caacaggcca tttatcagtt gatacagctg
     1501 caacggtgat tacaggtatt ggtgaaggtt gtttacaagc aggttgtgct ttagtcggtg
     1561 gcgaaactgc cgaaatgcca ggtatgtacg aaggcgaaga ttacgactta gcaggttttt
     1621 gtgtcggtgt tgtcgaaaaa tcacagatta ttgacggctc taaagtcaaa gcaggtgatg
     1681 tattaattgg tattgcttcg agtggtgttc actccaatgg ctattctttg gttcgtaaag
     1741 tgattgaggt tgctcaagcg gatttaagcc aaccattaga cggcaaaacc ttagccgatg
     1801 tggtgatggc acccacccgt atttatgtta aatcgatgtt gtctttaatt aaagctgtac
     1861 cagtacatgc gattgctcat attactggtg gcggtttacc aggtaattta ccacgtgtgt
     1921 tgccagaagg tgctgatgcg gtgattgatg agaaatcttg gcaatggcca gccattttcc
     1981 aatggttaca acagcaaggt aatgttgaac gttttgaaat gtatcgtacc tttaactgtg
     2041 gtgtgggcat ggttgtggtt gtgcctgccg ataaagccga tgaagccatt gctcatttac
     2101 aagccaatgg tgaacaagca tggcgtttag gtcaaattca gccnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnntttgaag gtaaattaat taatattcat ccctcgttat
     2281 tgcctttata taaaggtttg catacccatc aacgtgtatt ggcaacgggt gataaacttc
     2341 atggttgttc cgttcacttt gttaatgctg agttagatgg tggggcagtg attgcccaag
     2401 cggtgttaac ggtggcggct aacgaagccg aacaatcttt aatacaaaga gtacataaat
     2461 tagaacatat tatttatcct ctcgtgctaa attggcttag ttatggccgt ttgagttatc
     2521 aacaaggttt gcctgtgtta gatggtcaaa ccttgtctac accaatgaag ttgttatttg
     2581 attaaagttc tgcctctctc tgtgggagaa ggtttggctg aggggtaaat atgcgttttt
     2641 tgttgttaat cacgagttta agtcttagtg taaaaacctt tgcctttgat ttgcaacctt
     2701 atacagcaac gtatcaattt aatttaaatc aacaaattac tggcacagct acacgcacct
     2761 taagcaaaca agaaaacaat cattaccgtt atcaatttaa tgcaacagcc gctattgcca
     2821 atgctaatga agtgagtgag tttatttttg ataaacaaca agttcagtcg ttaagttatc
     2881 aaaatagtaa acaagtttta ttcaaaaatc gtgttgatca ggctactttt gattgggata
     2941 aacaacaatt aacaaccttg cgtaaaggcc aaaacgaaac atttccctta accgataaaa
     3001 cggttttaga ccctttaaat ttagaaatcc aaattcgcca agatttaagc cagcaaacta
     3061 aaccacaatt aaaagaatat ttattgggtg atgcaaaagg ttttcagcct ttaaagttta
     3121 ttgtggatgg tgttgaaaaa attaaaatcc cttatggcga aatagaagct ttaaaagtga
     3181 cgcgtattca tcagtcttcc gatagacaaa cgcagttttg gctagccaaa tccttagatt
     3241 atttgcctgt taaagtggtg caaatggatg atggtgcaat ttataccatt gaattgcaag
     3301 cattgcagaa gtgag
//
LOCUS       DAOQMB010000254         3314 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15527_length_3314_cov_15.449218, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000254 DAOQMB010000000
VERSION     DAOQMB010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15527_length_3314_cov_15.449218"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..244
                     /locus_tag="PLY05_08380"
     tRNA            168..244
                     /locus_tag="PLY05_08380"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Pro,seq:tgg)
     gene            265..341
                     /locus_tag="PLY05_08385"
     tRNA            265..341
                     /locus_tag="PLY05_08385"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:299..301,aa:Arg,seq:tct)
     gene            370..445
                     /locus_tag="PLY05_08390"
     tRNA            370..445
                     /locus_tag="PLY05_08390"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:His,seq:gtg)
     gene            607..819
                     /locus_tag="PLY05_08395"
     CDS             607..819
                     /locus_tag="PLY05_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005015822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03358.1"
                     /translation="MSSGKSEDFELDDAFDEESSGDFDNDDVSKSNNVKNSLTKRRVI
                     DDILEERRLQRKIKDYDYDLDDDDDK"
     gene            complement(857..1837)
                     /locus_tag="PLY05_08400"
     CDS             complement(857..1837)
                     /locus_tag="PLY05_08400"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNC03359.1"
                     /translation="MLSAQFLRHSLLLFVCLNISFAYGQAKKLSTITTPPNFKVAFIG
                     DQGLGLKSQQVLHLIKQEGAQAVIHLGDFDYYNQPQAWDEQINQILGKNFPYFAVIGN
                     HDLDKWPEYQQLLKQRMARIGLSWQGELGEQATITYNGLLMVMIGIGTKGDADVHNQY
                     LAQQLQQNNAIWRVAAWHKNQQAMQAGGKIDETGWQVYETARLQGAIIATGHEHSYSR
                     SYLLNNMQQQTIENKENTLKLSKGKSFVFVSGLGGIEERIQRQSGQHWAKIYAKTCLA
                     LDPVCLPNAKSGALFATFNVDKQANKAKFYFKNINQQIIDEFTVISEIKP"
     assembly_gap    1963..2062
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2067..>3314
                     /gene="dnaE"
                     /locus_tag="PLY05_08405"
     CDS             2067..>3314
                     /gene="dnaE"
                     /locus_tag="PLY05_08405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNC03360.1"
                     /translation="MSFVHLRLHSEFSLVDSIVRIGDAVKACQKANMPALALTDLSNF
                     YGLVKFYKAAQGKGVKPIFGADLWVAEDNGNHYLVTVLAMNEKGYFNLIEVISQSFIQ
                     GQQLGKAIVQREWLEQNNAGLIVLLGKQSYVGSLLLGADASSAIPVLQQWQSIFNDRL
                     YLELQRTSRTGDEAFVHAAVDLATKTDCPVVATNDVRFLTPDDFEAHEVRVCIAQGYV
                     LGDPKRPRDYSAEQYLKTPEQMIELFKDIPEAIENTLEIAKRCSVGLRLGKSFLPNYP
                     IPEGMTMDEFFIQISREGLEERLLSLLDKTQPNYEQQYKIYHDRLDFELKIINQMGFP
                     GYFLIVMDFIAWAKNNGVPVGPGRGSGAGSLVAYSLKITDLDPLKYDLLFERFLNPER
                     VSMPDFDIDFCMDGRDRVIDYVAN"
BASE COUNT          890 a          588 c          716 g         1020 t
ORIGIN      
        1 caagcattag tgcctttgca acgaatgtta gattttgcta agtcaaaacg ctgataaaat
       61 aagcagtcaa tcaaagagtt gaaaaaaaaa gttgacgaga gggactgata tttataatat
      121 acagccctcg ctgatgcaga ccaaatcaag tcggtgagtc aagtagtcgg ggcgtagcgc
      181 agtctggtag cgcatctgtt ttgggagcag agggtcgtag gttcaaatcc taccgtcccg
      241 accattttaa gtcaaatgag cctcgcgctc gtagctcagt tggatagagc atcggccttc
      301 taagccgagg gtcgcaggtt cgaatcctgc cgggcgcgcc atgtgtccag ccaaaaaaat
      361 ctctctatgg tggttgtagc tcagttggta gagccccgga ttgtgattcc ggttgtcgtg
      421 ggttcgagcc ccatcatcca ccccaattct ccttctaaat ccttaaaaat atcacctttc
      481 gtcagttttt ttgatttttc tatttacttt tctaaaatat tttagtatat agccgctcct
      541 tgtattgatg gcttaacttg tattgtgagc tgttttacga agatgcataa agattaagag
      601 gtgactatgt ctagtggcaa atcagaagac tttgagttag atgatgcttt tgatgaagag
      661 tcgagtggtg actttgacaa tgacgatgtc agtaaaagta ataacgtaaa aaactcttta
      721 actaagcgtc gtgtcattga tgatatttta gaagagcgtc gtttgcagcg taaaatcaaa
      781 gattatgatt acgatttaga cgatgatgat gacaaataaa gaaaacccgt gaaaacgggt
      841 ttttttgtgc aaaaaattaa ggtttgatct cactaatcac tgtaaattca tcaataatct
      901 gttggttgat atttttaaag taaaatttgg ctttattggc ctgtttatcg acattaaagg
      961 tcgcaaacaa tgcccctgat ttagcattcg gcaaacacac agggtctaaa gctaaacaag
     1021 ttttggcata tatttttgcc caatgttgac ctgattggcg ttgaatccgt tcttcaatgc
     1081 cgcctaagcc tgatacaaag acaaagcttt tacctttgct tagctttaag gtattttctt
     1141 tattttcaat cgtttgctgc tgcatattgt tgagtaaata gctgcgtgaa taagagtgtt
     1201 cgtggcctgt tgcaatgatt gctccctgta aacgtgctgt ttcataaact tgccagcctg
     1261 tttcgtcaat cttgccacct gcttgcatgg cttgttggtt tttatgccat gcagcgacac
     1321 gccaaatcgc gttgttttgt tgtagttgtt gcgccaaata ttgattatgc acatcagcat
     1381 cgccttttgt gccaatacca atcatcacca tcaataaccc attgtaggta atggttgcct
     1441 gttcacctaa ttctccttgc catgacaggc cgatacgtgc catccgttgt ttaagcagtt
     1501 gttgatattc tggccatttg tctaaatcat ggttgccaat cacggcaaaa taaggaaagt
     1561 ttttgcctaa aatttgatta atttgctcat cccacgcttg gggttggttg tagtaatcaa
     1621 aatcacctaa atgaataacc gcttgtgcgc cttcttgttt tatcaagtgc aaaacttgtt
     1681 gagatttcag gcctaaacct tgatcaccaa taaacgccac cttgaagttg ggaggagtgg
     1741 tgattgttga taatttttta gcttggccat aagcaaagct aatgtttaaa cagacaaaca
     1801 gaagtaggct atgacgcaaa aattgagctg ataacatggt tgtgatcaaa tagttgttgt
     1861 tgtcgagttt taatatagga cttaggtttt tcaccgaaat tagtgcgtaa gtcctaaata
     1921 atagtcacaa aaattgtctg attgatgatt ttcgcgtact atnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnctttatga gctttgtcca tttgcgctta cacagtgaat
     2101 tttccttggt agattctatc gtacgcattg gtgatgccgt caaagcgtgt caaaaagcca
     2161 atatgcccgc tttggcgttg acggatttaa gtaattttta tggtttggta aagttttata
     2221 aagcagcaca aggtaagggt gtcaagccga tttttggtgc agatttatgg gtggccgaag
     2281 acaatggtaa tcattatctc gtcactgtct tggcgatgaa tgaaaaaggc tattttaatt
     2341 taattgaagt gatttcccaa agttttattc agggacagca attaggcaaa gcgattgtgc
     2401 aacgggaatg gttggaacaa aacaatgcag gtttaatcgt tttattaggc aaacaaagtt
     2461 atgttggttc gttgttatta ggtgcagatg ccagttcggc tattcctgtg ttgcagcagt
     2521 ggcaaagcat ttttaacgat cgattatact tagaattaca acgtacaagt cgtacaggtg
     2581 acgaagcctt tgtccatgcg gcggttgatt tagcaacaaa aaccgattgc ccagtggtgg
     2641 caaccaatga tgtacgcttt ttaacacctg atgattttga ggcgcatgaa gtacgggtgt
     2701 gtattgccca aggttatgtg ttgggtgatc ccaaacgtcc acgagattac agtgccgagc
     2761 agtatctaaa aacacctgaa caaatgatcg agttgtttaa agatattcct gaagcgattg
     2821 agaatacgct agaaattgcc aaacgttgtt cggtaggctt acgtttaggt aaaagctttt
     2881 tgcctaatta tcccattccc gaaggtatga ccatggatga gtttttcatc caaatttcgc
     2941 gcgaaggttt agaggaacgt ttacttagtc tattagataa aacgcagcca aactatgaac
     3001 agcaatataa aatttaccat gatcgcttag actttgagtt aaaaattatc aatcaaatgg
     3061 gtttccccgg ttactttttg attgtgatgg actttattgc atgggcaaaa aataacggtg
     3121 tccctgttgg gccgggtcgt ggttcggggg ctggttcttt ggtggcctac tcactcaaaa
     3181 ttaccgactt agacccgtta aaatacgatt tgctgtttga acgttttttg aatccagaac
     3241 gggtgtcgat gcctgacttt gatatcgact tttgtatgga tggtcgagac cgcgtgattg
     3301 attatgtggc caat
//
LOCUS       DAOQMB010000255         3289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15708_length_3289_cov_14.580705, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000255 DAOQMB010000000
VERSION     DAOQMB010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15708_length_3289_cov_14.580705"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="purH"
                     /locus_tag="PLY05_08410"
     CDS             <1..943
                     /gene="purH"
                     /locus_tag="PLY05_08410"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003659336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HNC03361.1"
                     /translation="QFIKAQDLRYGENPHQKAAFYVEAQPKEASIATAKQLQGKELSY
                     NNIADTDAALECVKSFVKPACVIVKHANPCGVAVSLDGIKAAYDLAYQTDTESAFGGI
                     IAFNRELDAETAKAIVERQFVEVIIAPKVSAEALAITAAKQNVRVLECGQWSAERSQQ
                     LDYKRVNGGLLVQDLDIGMITEGDLKVVTKRAPTEAEIHDLIFAWKVAKFVKSNAIVY
                     AKGRQTIGIGAGQMSRVNSARIAAIKAEHAGLQVQGAVMASDAFFPFRDGIDNAAKVG
                     ISCVIQPGGSMRDAEVIAAADEHGMAMVFTGMRHFRH"
     gene            1000..2295
                     /gene="purD"
                     /locus_tag="PLY05_08415"
     CDS             1000..2295
                     /gene="purD"
                     /locus_tag="PLY05_08415"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004654484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNC03362.1"
                     /translation="MNILILGSGGREHALAWKCAQDPRINTVFVATGNAGSATEAKCQ
                     NVAINMLDNQALLNFAQENNVELTIVGPEAPLVNGVVNAFRAAGQKIWGPTQYAAQLE
                     GSKAFAKDFLARHQIPTAYYGVFTDTHQALAYVREKGAPIVIKADGLAAGKGVIVAMT
                     LDEAEAAVTDMLSGNKFGSAGARVVIEEFLDGEEASFICMVDGVNALAMATSQDHKRI
                     GDADTGPNTGGMGAYSPAPVVTPEVHARVMREVIMPTVQGMAAEGHPYTGFLYAGLMI
                     DATGAPKVIEFNCRFGDPETQPIMMRLQSSLVELVEAGLAQQLPTEAQWDARPAVGVV
                     MAAGGYPNDVRKGDVIKGLDILHTHSKVFHAGTSLENGEVVTNGGRVLCVTALGKTVS
                     EAQANAYRHVQEITWADEYHRTDIGYRAIAREQQAQQQQ"
     gene            complement(2378..3172)
                     /locus_tag="PLY05_08420"
     CDS             complement(2378..3172)
                     /locus_tag="PLY05_08420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC03363.1"
                     /translation="MSGHRRTALITGASAGIGATFARFLAAQGYDLVLVARRVERLNE
                     LAKELNQQFGIKSYVFAADLTDKQAPTAIMAFVKEQSVVIDMLINNAGLSGKTAFADT
                     PWQDLANEIQLMITAVTELAYRVIPDMKQQSWGRIINLSSLAAFSPPGASLLYTGIKS
                     YVLNISESLDMELKPYGIHVTALCPGFTHSEFHDVMGTRDTANHLPAILWQQPEAVVQ
                     EGYEAVMKGKPVCVPGIVNKVIASSMRPIPESARYFLGKTFNPFKN"
BASE COUNT          926 a          695 c          732 g          936 t
ORIGIN      
        1 ccagtttatt aaagcgcaag atttacgtta tggtgagaat cctcatcaaa aagccgcttt
       61 ttatgttgaa gctcaaccca aagaagcgag cattgccacc gcaaaacaat tgcaaggcaa
      121 agaattatct tataacaaca ttgctgacac ggatgcagcc ttagaatgtg ttaaatcctt
      181 tgtgaagcca gcttgtgtga tcgtcaaaca cgccaaccct tgtggtgttg ctgtgtcctt
      241 agatggtatt aaagcggctt atgacttagc ttaccaaacc gatactgagt ctgcttttgg
      301 tggcattatt gcctttaacc gtgagttaga tgccgaaact gccaaagcga ttgtcgaacg
      361 tcaatttgtt gaagtgatta ttgcaccaaa agtcagtgcc gaagctttag ccatcactgc
      421 tgctaaacaa aatgtgcgtg ttttagaatg tggtcaatgg tctgctgaac gtagccaaca
      481 attagattac aaacgtgtta atggtggttt gttagtacaa gatttagata ttggcatgat
      541 taccgagggt gacttaaaag tagtcactaa acgcgcccct accgaagccg aaattcatga
      601 tttaattttt gcatggaaag tggccaaatt tgttaaatcc aatgccattg tttatgccaa
      661 aggccgtcaa actattggta ttggtgcagg ccaaatgagc cgtgttaact ctgcgcgtat
      721 tgctgcgatt aaagccgaac atgcaggctt gcaagtacaa ggtgcagtca tggcttctga
      781 tgccttcttc ccattccgtg atggcattga taatgctgct aaagtgggca ttagctgtgt
      841 aattcaacct ggtggctcta tgcgtgatgc cgaggtgatt gccgctgctg atgagcatgg
      901 catggcgatg gtgtttacag gtatgcgcca cttccgtcat taataattgg ccattaaagg
      961 cttggttatc caagcctttt ttgtttttga ggattaaaaa tgaatattct tattttaggc
     1021 tcaggtggcc gcgaacacgc ccttgcttgg aaatgtgccc aagacccacg tattaatacc
     1081 gtttttgttg cgacaggcaa tgcaggctca gccactgaag ccaaatgcca aaatgtggcg
     1141 attaatatgc ttgataacca agccttatta aattttgccc aagaaaataa tgtcgaatta
     1201 accattgttg gcccagaagc tcccttggtt aatggtgtgg tgaatgcttt tagagcagca
     1261 ggccaaaaaa tttgggggcc tactcaatac gcagcacaat tagaaggctc aaaagccttt
     1321 gccaaagact ttttagcccg tcatcaaatt ccaacagcct attacggtgt atttaccgac
     1381 actcatcaag ccttagccta tgttcgtgaa aaaggcgcac cgattgtgat taaggcggat
     1441 ggtttagccg caggcaaagg cgtgattgtc gccatgacct tagacgaagc cgaagccgcc
     1501 gtaacagata tgctatctgg caataagttt ggcagtgctg gtgcgcgtgt ggtgattgaa
     1561 gagtttttag atggcgaaga agccagtttt atttgtatgg ttgatggggt taatgcctta
     1621 gccatggcga ctagccaaga ccataaacgt attggtgatg ccgatacagg accaaacaca
     1681 ggcggtatgg gtgcttattc acctgcacct gtggttacgc ctgaagtgca tgctcgtgtg
     1741 atgcgtgaag tgattatgcc cactgttcaa ggtatggcgg ctgaaggtca tccatatact
     1801 ggctttttgt atgcgggttt aatgattgat gcaacaggcg caccaaaagt tattgagttt
     1861 aactgtcgtt ttggtgatcc tgaaacacaa ccgattatga tgcgtttgca atcttcatta
     1921 gtggaattag ttgaagcggg tttagctcaa caattaccca ccgaagctca atgggatgct
     1981 cgccctgcgg tgggtgtggt catggctgcg ggtggttatc caaatgatgt gcgcaaaggc
     2041 gatgtcatta aaggtttaga tattcttcat actcacagca aagtctttca tgctggtact
     2101 agcttagaaa atggtgaagt cgtcactaat ggtggtcgtg tgttatgcgt aactgcttta
     2161 ggcaaaacgg taagtgaggc acaagccaat gcttatcgtc atgtacaaga gattacatgg
     2221 gctgatgaat atcatcgtac ggatattggc tatcgtgcta ttgctcgtga acaacaagca
     2281 cagcaacagc agtaagcatt ttaagtatgg tgagtgaaag tcgtggtggt tgagcggatc
     2341 gaaaccactt cgactccgct cagtgtgcat aattgcttta attcttaaac ggattaaacg
     2401 tttttcccaa aaagtatcgt gctgactcag gaatggggcg catactactg gcaatcactt
     2461 tattgacgat acctggtaca cacactggct tccctttcat caccgcttca taaccctctt
     2521 gcactacagc ttctggttgt tgccacaaaa tcgcaggcaa atgatttgct gtatctcgtg
     2581 tccccatcac atcatgaaac tcactatggg taaaaccagg acataaggcc gtgacatgaa
     2641 tcccataagg ctttaattcc atatctaatg attctgaaat attcaagaca taacttttaa
     2701 tgcctgtgta taacaagctt gcacccggtg gagaaaaggc cgccaatgac gataaattaa
     2761 taattctacc ccaactttgt tgtttcatat caggaatcac acgatatgct agctcggtca
     2821 ccgctgtaat catcaattga atttcattgg ctaagtcctg ccaaggcgta tcagcaaaag
     2881 ccgttttacc tgataaaccc gcattattaa ttaacatatc aatcacaacg ctctgctctt
     2941 taacaaaagc cataatcgct gtaggtgctt gtttatctgt taaatctgcc gcaaatacat
     3001 aacttttaat accaaattgc tgattgagct ctttagctaa ctcatttaag cgctcaacac
     3061 ggcgagcaac caaaaccaaa tcataacctt gtgctgctaa aaaccgcgca aaggttgcac
     3121 caatacctgc tgatgctcct gtaattaatg ccgtacggcg atgaccactc ataaaacacc
     3181 tcttattaag ctgataacca caatcttgaa cagaattcaa gataacttac tcttgaataa
     3241 caatcaagaa gaatctgact atgatttatg ctcagatttt tctacactg
//
LOCUS       DAOQMB010000256         3267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15903_length_3267_cov_17.333748, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000256 DAOQMB010000000
VERSION     DAOQMB010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15903_length_3267_cov_17.333748"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLY05_08425"
     CDS             <1..500
                     /locus_tag="PLY05_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03364.1"
                     /translation="IDIVKKLPPDMLQSKYQSMLAGTPLNKYLGNPEQQKAITDYNLM
                     LENLRLYIEKPAGAISNYEQGILGRAIGILEGNYSNEQKESALKRVKGIFDNQKNVAE
                     NVLKQNKAERFNYNLQKDKPLRFTKQANSQQRREYGEAIFAAKGDPELIREIQEHARE
                     LGVLE"
     gene            500..>1813
                     /locus_tag="PLY05_08430"
     CDS             500..>1813
                     /locus_tag="PLY05_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03365.1"
                     /translation="MGDYDHILKKSQPTKTVEKTKQSEPSDAAVLASSLAKGVAGIPD
                     MFVNAAPNLVGLGMAGSGFVSNELAERLGRDTTLGGYFSKVNELANQGLSNFAYAPSI
                     ANKALRAVAPIQDPVTPSQRVIDWAAQSATGALLTPAASVKDFALNAAKFGGYGGLGG
                     VVAEETGSPVAGIAASIAAPVALNAGANLASRINQPFTVGGRENIVGQRLVNIVQDPV
                     AASAALKNAPKPLVKGSQATSGQVLAQQTGDKNLLRAEDVVRQKIGIADDINARYAQN
                     QAARQAEIARLGGGGTAQDAVTIAREKLAQNKLILEQQKARLMQSQQARGQQAAQQQQ
                     TALSDLGSPTSADDAAQRLASVADPILAQKQKDIGNAFESVDPFDEVKALRIPMAKIE
                     GILQNNYGGLSLAQDSKVRQAIKLINEAASQNKAAKDIPELAIVKP"
     assembly_gap    1814..1913
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1914..3251
                     /locus_tag="PLY05_08435"
     CDS             <1914..3251
                     /locus_tag="PLY05_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03366.1"
                     /translation="DLELFNENLKLNPDLLGSTMTYKQMQTIQSRIGQLQREAIKSGS
                     SVDAKTLTELKHLIRGTMDDEVKRGVVPADIAESYKKAIGLKADFEKRYGSAPISDLR
                     FRGYERQSQNLATLPKKLVSTREGLESFKKSFGSEKEARQALSDYIASDFRTQVLNIG
                     NGGLKSGWEEKAFKFIKDNKLALDEFGDLSKKIMFAIQKSKNTQQLQKSFDDELTNLA
                     KKYALRESKQLEQGGKVFNNLKDPDKVIDAFFSSSMRENDAKFIIELASESKVFNRAL
                     RGAISDKINKLNDNQMIKFLEDSKNIKLIESLYGKSTSSALNKVLQDAKRDAMGRTGL
                     GSGSQTFGRGQIDKLMNQNIYALDALGGTLGGLVGSLLGNPILGVVGGASVAHKTKQI
                     TMALDEQMAKAFLNSDEAARLIDKKLSSGNYLKNAKSTATKNIVPASQNEDKK"
BASE COUNT         1077 a          503 c          695 g          892 t
ORIGIN      
        1 ctattgatat tgtaaaaaaa ttgcctcctg atatgttgca atcaaaatat cagtccatgc
       61 ttgctggcac tccattaaat aaatatttgg gcaatcctga acagcaaaaa gcaataacag
      121 attataactt gatgcttgaa aatttacgtt tgtatatcga aaagcctgct ggtgcgattt
      181 ctaactatga gcaaggtatt ttaggtcgtg ctattggtat tttagagggt aattattcaa
      241 atgaacaaaa agagtctgca ttaaaacggg tgaaaggcat atttgacaat caaaaaaatg
      301 tagccgaaaa tgttttaaag caaaataaag cagagcgttt taattataat ttacaaaaag
      361 ataagccgtt aagatttaca aaacaggcca actcacagca aagacgagaa tatggcgaag
      421 ctatttttgc cgcaaaaggt gatcctgagc taatcagaga gattcaagaa cacgcgcgcg
      481 aattgggagt gcttgaataa tgggcgatta tgaccacatt ttaaaaaaat cacaaccgac
      541 aaagacagta gaaaaaacaa aacagagtga gcctagtgat gctgcggttt tggcttcatc
      601 tttggccaaa ggtgttgctg gcatacctga tatgttcgta aatgctgcgc ctaatcttgt
      661 cggcttaggt atggctggta gcggttttgt atctaatgaa ttggctgaaa gattagggcg
      721 agatacaaca ttaggcggtt atttctcaaa agtaaatgaa ttggctaatc aagggttatc
      781 taactttgct tatgcgccaa gtattgcaaa taaagcatta agggctgtcg ctcctattca
      841 agaccctgtt acgcctagtc aaagagttat tgattgggct gctcaaagtg ccacaggtgc
      901 attactgact cctgcagcta gtgtaaaaga ctttgcatta aatgcggcta aatttggcgg
      961 ttatggtggc ttgggtggtg ttgtcgctga ggaaactggc tcacctgttg cgggtattgc
     1021 cgcgtcaatt gctgcgcctg ttgcgttaaa tgcgggtgct aaccttgcat caagaattaa
     1081 tcagcctttt acggttggcg gtagagaaaa tatagtaggt caaagacttg tcaatattgt
     1141 gcaagaccct gtagcggcta gtgctgcttt aaaaaatgcg cctaagccat tggttaaagg
     1201 ttcgcaagca acaagtggcc aagtattagc acaacaaaca ggtgataaaa acctattaag
     1261 agccgaagat gttgtaaggc aaaaaatagg tattgctgac gatattaacg cacgttatgc
     1321 acaaaatcag gctgcaaggc aagctgagat tgctcgttta ggtggtggtg gtactgctca
     1381 agatgcggtt acgattgcgc gtgaaaaatt agcacaaaac aaattgattt tagagcaaca
     1441 aaaagcgcgt ttaatgcaat ctcaacaagc aagaggccaa caggcagccc aacaacaaca
     1501 aacggcatta agtgatttgg gttctccaac aagtgcagac gatgcagcgc aaagattggc
     1561 tagtgttgct gatcctattt tagctcaaaa acaaaaagac attggcaatg cttttgaaag
     1621 tgttgaccca tttgacgagg taaaagcact tcgcatacct atggcaaaaa tagagggtat
     1681 tttacagaat aattacggtg gattatcgct tgctcaagat agtaaagtta gacaagccat
     1741 taaactaata aatgaggcag ctagccaaaa taaagctgct aaggatattc cagaattggc
     1801 aattgttaag ccannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngatttag
     1921 aattgtttaa tgaaaaccta aaacttaatc ctgatttgct tggttcaacg atgacctata
     1981 agcaaatgca aactattcaa agcagaatag ggcaattaca aagagaagct attaagagtg
     2041 gttctagtgt tgatgctaaa acattaacag agttaaagca tttaatacgc ggcaccatgg
     2101 atgatgaagt taagcgcgga gttgttcctg ctgatattgc cgagtcatat aaaaaagcaa
     2161 taggcttaaa ggctgatttt gaaaaacgtt atggttctgc gcctatttct gatttacgtt
     2221 ttaggggtta tgagcgtcaa agtcaaaatt tagcaacatt acctaaaaaa cttgtgtcaa
     2281 ctcgtgaggg tttggagtct tttaaaaaat cttttggcag tgaaaaagaa gcacgtcaag
     2341 cgttaagtga ttacattgca tccgatttta gaacacaagt tttaaatatt ggtaatggtg
     2401 gattaaagag tggatgggaa gaaaaggcat ttaagtttat taaggataac aaattagctt
     2461 tagatgaatt tggtgattta tctaaaaaaa tcatgtttgc tattcaaaag tcaaaaaaca
     2521 cacaacaatt gcaaaagtca tttgatgatg aattgactaa tttggcaaaa aaatatgctt
     2581 tgcgtgaatc taagcaatta gagcaaggcg gcaaagtgtt taataaccta aaagaccctg
     2641 acaaggttat tgatgctttt ttttcgtcat ctatgcgcga aaatgacgct aaatttatca
     2701 ttgagttggc aagtgaaagt aaggttttta atcgtgcttt gcgtggtgca atatccgaca
     2761 aaataaataa attaaatgac aatcaaatga tcaaattttt ggaggattcc aaaaacatca
     2821 aattgattga atcattgtat ggcaaaagca catcaagcgc attgaataaa gtgcttcaag
     2881 atgctaaacg agatgcaatg ggtcgtactg gtttaggttc aggttcacaa acatttgggc
     2941 gcggtcaaat tgataaatta atgaatcaaa atatttacgc gcttgatgct ttaggcggta
     3001 cacttggcgg attagttggc tcattattag gtaatccaat tctaggtgtt gtaggtggtg
     3061 ctagtgttgc acataagact aagcagatta caatggcatt agatgaacag atggcaaagg
     3121 cgtttttaaa tagtgatgag gctgctaggt tgatagataa aaagttatct agcggtaatt
     3181 atttaaaaaa tgcaaaatca acagctacta aaaatattgt tccagcttct caaaatgagg
     3241 ataaaaaata atgtcttatt cactttc
//
LOCUS       DAOQMB010000257         3266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15914_length_3266_cov_16.431330, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000257 DAOQMB010000000
VERSION     DAOQMB010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15914_length_3266_cov_16.431330"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="PLY05_08440"
     CDS             complement(<1..212)
                     /locus_tag="PLY05_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03367.1"
                     /translation="MTRLKFLALSISLFTAACSPHRLIGNQINHFSQENVLPVVMASD
                     DITMVCHANETNTPLLMSFNKFGVDS"
     gene            453..1412
                     /gene="miaA"
                     /locus_tag="PLY05_08445"
     CDS             453..1412
                     /gene="miaA"
                     /locus_tag="PLY05_08445"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005405518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNC03368.1"
                     /translation="MQNLFVESENKPVILLMGPTASGKTDLAIRLAQEFPVEIISVDS
                     SMVYTGLDIGTAKPTQSELTLAPHRLIDIRDPAQPYSAADFRSDALAHIAEIHQQGRI
                     PLLVGGTMLYFKVFKDGLANLPQANEEIRQKILLQAQEQGWPAIHQLLVQVDPITAQR
                     LKPTDAQRLQRALEVYYVTGVPLSTWHAQQQREELPFQYYQFALLPKAREILHQRIEL
                     RFDKMLEQGFIEEVIRLKQREDLNLDLPSMRSVGYRQVWEYLDKQYDFDEMRFRGIVA
                     TRQLAKRQHTWLRSLEHDVHQLDTEDAWIVLQKCLKKIIFSRK"
     gene            1565..1786
                     /gene="hfq"
                     /locus_tag="PLY05_08450"
                     /pseudo
     CDS             1565..1786
                     /gene="hfq"
                     /locus_tag="PLY05_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
     gene            2257..>3266
                     /locus_tag="PLY05_08455"
     CDS             2257..>3266
                     /locus_tag="PLY05_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC03369.1"
                     /translation="MSRWQRLRTTPEDKKAAAIAQLQQQLQQSQQAVAQRQQALPAVT
                     LNEDLPVSQRAQEIIQAIQQHQVVVLAGETGSGKTTQLPKLCLLAGRGVYGFIGHTQP
                     RRLAARSVAARIAEELNSPLGEVVGYKVRFNEACKAESYIKLMTDGILLAELANDRYL
                     NAYDTLIIDEAHERSLNIDFLLGYLRQLLAKRPDLKVIITSATLDVERFSRHFNGAPI
                     IQVEGRTFPVEVRYRPMAEESVQSDEDEGFELLEEAMPRAVVNAVEECIEHSRQQGRA
                     GSGDILVFASTEREIRDLADTLRKYGPPHTEILPLYARLSLNEQQRIFHPTGKGRRII
                     IAT"
BASE COUNT         1008 a          592 c          687 g          979 t
ORIGIN      
        1 ctactatcaa cgccaaattt attaaatgac attaataatg gcgtattggt ttcgttagca
       61 tgacaaacca tcgtgatatc atcactggcc atgacaacgg gtaacacgtt ttcttgtgaa
      121 aaatgattga tttgattacc aattaagcga tgtggtgagc aagcagcagt aaataagctg
      181 atacttaatg ctagaaactt aaggcgagtc atggataagt tatcccatat ttaaaaggca
      241 ctttaggcac ttttaggtgt tgttatagtg ttttattcta tgagtatctg cgaaaattgt
      301 tggtttatgg cgtatatcgc ttgatacttg ttcaatggag ttaatgtaat acgacttgat
      361 tgtaatcgca attatatcat gatgattaag tccgaaaaaa tgtgatgttg tctgtatttt
      421 atacaccttt aaaattgaga gaattttaat taatgcaaaa tttatttgtt gaatcagaaa
      481 ataaacccgt tattttatta atggggccta ccgcaagtgg caaaacagat ttagcaattc
      541 gcttggcaca agagtttcct gtagaaatta tttctgttga ctctagtatg gtctatacag
      601 gtttagatat tggtacagca aaacccactc agtcagagtt aacattagca ccacaccgct
      661 taattgatat tcgtgatcct gcccaacctt attccgcagc agattttaga agtgatgctt
      721 tagcacatat tgcagagatt caccaacaag gacgaatccc cttattagtg gggggaacga
      781 tgttatattt taaggtgttt aaggatggtt tagccaattt gcctcaagcc aatgaagaaa
      841 tcagacaaaa aatcttatta caagcacaag agcaaggttg gcctgctatt catcaattat
      901 tggtacaagt tgaccccatc accgcacagc gtttaaagcc gacagatgcc caacgtttac
      961 aacgtgcctt agaagtttat tatgtgacgg gtgtgccttt atcaacatgg catgcacaac
     1021 aacaacgaga agaattgcca tttcaatatt atcagtttgc cttattgcca aaagccagag
     1081 aaattttgca tcaacgtatt gaattacgtt ttgataaaat gttagagcaa ggttttattg
     1141 aggaagtaat acgtttaaaa caacgggaag atttaaactt agatttaccc tctatgcgct
     1201 cagtgggtta tcgtcaagtt tgggaatatt tagacaaaca atatgatttt gacgagatgc
     1261 gttttcgtgg tattgtggca acgcgtcagt tagccaaacg tcaacataca tggttgcgta
     1321 gtttagaaca cgatgttcac cagcttgata cagaagatgc ttggattgtc ctacaaaaat
     1381 gcttgaaaaa aattatattt agccgaaaat gagcagcagg tttaattgac tttcaattgc
     1441 cttagagtgg ggttgtccga gtaattaaaa agatttgtat taagcggcta acggcactct
     1501 gttttttatt ttgttttttg cactaattaa attttatctg ccgttttggc agtggagacg
     1561 aatcatgtct aaagggcaaa ccttacaaga cccgttttta aatgcgctgc gtaaagagcg
     1621 tattcctgtg tctattttct tggttaatgg cattaaatta caaggtcaaa tcgaatcttt
     1681 tgatcaatat gtcgtgttat taaaaaacac agttagccaa atggtttaca aacacgcgat
     1741 ttctaccgtt gttccatccc gtaacccacg tccagctggc gcaccaaatg caccaattgg
     1801 cccaatgacc agtggtacaa tgggaacagg ttatccatat gatgatcaaa tggacgatca
     1861 agacttaggc tattaatctc gtaagagcct agtctgtact ttaaaaaagg cttcatggtt
     1921 agcgtgaagc ctttttgtat tttagaagat taggacttac gcattttgca aaattagcgc
     1981 gttttgtgag cataaaaccg ttgaaaatgg ttgaaatgtg aacaaataag cgataaccgc
     2041 gtaagtccta agatgatgat agtaaaattg tgtgtaaagt aaaaaaatag agatcaatag
     2101 caatttaacg gcttaacaat tttgaaatac gattagaccc tacgttaatc agtcaaaaat
     2161 taaatataag ccttgaattt ttaagaatta tgtatgcaaa aacaccaatt tttttctgct
     2221 ttagataaaa gtcttactca agaccgtcca cgttttatga gtcgttggca gcgtttacgt
     2281 accacacctg aagacaaaaa agctgccgct attgctcagt tacagcaaca gttacaacaa
     2341 tctcaacaag cagtggcgca acgtcaacag gctttgcccg cagtgacctt gaatgaagac
     2401 ttacctgtta gccaacgtgc gcaagaaatt attcaagcca ttcaacaaca tcaagtggtg
     2461 gttttagctg gcgaaacagg ttcaggcaaa accacgcaat tacccaaatt atgtttattg
     2521 gcagggcgtg gtgtgtatgg atttattggc catacccaac ccagacgttt agcggcgcgg
     2581 agtgtggcag cacgtattgc cgaagaatta aactcaccgt tgggcgaagt ggttggttat
     2641 aaagttcgtt ttaatgaagc gtgtaaagcc gagtcgtata tcaagttaat gactgacggt
     2701 attttgttgg cggaattagc caatgaccgt tatctaaatg cgtatgacac gctcattatt
     2761 gatgaagcac atgaacgtag cctaaatatt gattttttat tgggttattt gcggcagtta
     2821 ttggccaaac gtcctgattt aaaagtgatt attacctccg caaccttaga tgttgagcgg
     2881 tttagtcgtc attttaatgg tgcgccaatt attcaagtgg aaggacgtac ctttcctgtg
     2941 gaagtgcgtt atcggccaat ggctgaagaa agcgtacaat ctgacgaaga tgaaggcttt
     3001 gagctacttg aagaagctat gccgcgagcg gtggttaacg cggtagaaga atgtattgaa
     3061 cactcacgcc aacaggggcg agcaggtagc ggtgatattt tggtgtttgc cagtaccgaa
     3121 cgtgaaattc gcgatttagc cgatacctta cgcaaatatg gcccaccaca taccgaaatt
     3181 ttgcccttat atgcacgttt atcgcttaac gagcaacaac gtatttttca tccgacaggc
     3241 aaaggccgac ggattattat tgccac
//
LOCUS       DAOQMB010000258         3258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_15973_length_3258_cov_16.037777, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000258 DAOQMB010000000
VERSION     DAOQMB010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15973_length_3258_cov_16.037777"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..434)
                     /locus_tag="PLY05_08460"
     CDS             complement(105..434)
                     /locus_tag="PLY05_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03370.1"
                     /translation="MAGLKGLKKGDRVDLEIQAESFISGAKERIDALSITPTTDKVVE
                     TKERNRVFERYTFSLNPNVSATIDTISMLPRHFKVNRSEVVRVAIEYLNTLPEAQLIE
                     ILQKAKQ"
     gene            complement(436..1074)
                     /locus_tag="PLY05_08465"
     CDS             complement(436..1074)
                     /locus_tag="PLY05_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004898139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03371.1"
                     /translation="MILLVGGEKGGSGKSCLAQNIAVYLQHRQKDVLLLDADPQGTTT
                     DWAKEREENDGLTNLPCVQASGNIRQILKDLAKRYQVIVVDAGGQDSEALRSAMTVAT
                     HLLLPFRPKRRDLKTLVHVSQLVKLAKAVNPDMLVRGVITQCPTLPSQTQRILDAKEA
                     CQSFGIEPLQSITCNRNVYDDADENGSSVLEVETDLKAKEEVESLVAEFLEV"
     assembly_gap    1214..1313
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1445..2308)
                     /locus_tag="PLY05_08470"
     CDS             complement(1445..2308)
                     /locus_tag="PLY05_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HNC03372.1"
                     /translation="MNDNSNNQLIVKSNRLIEASYRLTLNEQRIILLAISRVRRDREL
                     QLDDVFEVHASDLVSYFGVDPKTSYSELIDVSKTLFNRHVTLENPYPNDPRVKTLLTR
                     WVSSIAYLPEHGCIRLRFSQDVLPFLSLLETQFTRYKLEVVGKMTSIYAVRLYELLIQ
                     WGSVGKREIEIEWLKQRFQIEDKYPAIKDLKKYVIDVAVAQINQHSDMNVTYTQRKTG
                     RVVSHLIFAFELKKSVVPKKAKSLKLKNNIDASAPETIFGVLKADIEKLARPGESYED
                     AASRIKKMNLS"
     gene            complement(2570..2854)
                     /locus_tag="PLY05_08475"
     CDS             complement(2570..2854)
                     /locus_tag="PLY05_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018679947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03373.1"
                     /translation="MLLCSNPLFSLGQTVATPNALDLLAQHNTSCFSLLARHQSGDWG
                     NVPAEDALSNQQAIERGYRIMSVYPLETGNVWIITEADRSVTTLLLPEEY"
     gene            complement(2879..>3258)
                     /locus_tag="PLY05_08480"
     CDS             complement(2879..>3258)
                     /locus_tag="PLY05_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase or metal-binding protein"
                     /protein_id="HNC03374.1"
                     /translation="GKSTTQSHRSAIYYVDLTLRDNHTLAEALQQAKTLNQQRLEIGF
                     QQQALDQAAEEGFNNAQFEFSEEEIPELLEEFYPNPDDDNSAQGQSTSNNPNNTSSTP
                     KTANGVLKQKLGQRLQAQSTSTN"
BASE COUNT          970 a          631 c          499 g         1058 t
ORIGIN      
        1 gttatgcaag ttatgcaagt tatgcaagtt atgcaagtta tgcaagtttg tttgaaatca
       61 gtttaatgtc aacattcttt cgcttattcg cttacaccag aaacttattg cttagctttt
      121 tgtaaaatct caatcaattg tgcttctggc aaagtgttta aatattcgat agcaactctt
      181 acaacctctg atcggttcac cttaaaatga cgaggtagca tagaaatagt atctattgtt
      241 gcgctcacat taggatttaa cgaaaaagta taacgctcaa aaacgcggtt tctttctttg
      301 gtctcaacaa ctttatctgt tgtcggtgtt attgatagag catcaattct ttctttagct
      361 ccactaataa aactttcagc ctgaatttca agatcaacac gatcaccttt ttttaagcct
      421 tttaaacctg ccatattaaa cctctaaaaa ctcagcgact aacgattcaa cttcttcttt
      481 agcttttaaa tcagtttcta cttctaaaac agaagatcca ttttcatcag catcatcata
      541 aacattacga ttacaggtaa ttgattgaag tggttcaatc ccaaaagatt ggcaagcctc
      601 tttagcatct aaaatacgtt gtgtttgact gggcaaagtt ggacattggg taattacacc
      661 acgaaccaac atatctggat ttactgcttt cgcaagtttt accaattggc tcacatgaac
      721 taaagttttt aagtcacgtc tcttgggtct aaaaggtaat aataaatgag tagcaactgt
      781 cattgctgag cgtaacgctt ctgagtcctg accaccagca tccaccacaa taacctgata
      841 acgcttggct aaatctttta aaatttggcg aatattaccc gatgcttgca cacatggaag
      901 attggtcaat ccatcatttt cttcacgttc tttagcccaa tccgttgttg ttccttgagg
      961 atcagcgtct aataacaaaa catctttttg acgatgttgc agataaactg caatattttg
     1021 agcaagacaa ctttttcctg aaccgccttt ttctccaccc accaataaaa tcatggtcta
     1081 agtctccaag ttttttattt gccaatttga tgaaaatttt ctagttatca ctcaaagtat
     1141 gataacacca acttacttaa cttacttaac ttacttaact tacttaactt acttaactta
     1201 cttaacttac ttannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncttactt
     1321 aacttactta acttacttaa cttacttaac ttacttaact tacttaacaa ggagtgtata
     1381 taattatcac cttgaatacc gtcttttcct ccctttatac tatttaagtt attgatatta
     1441 aaagctaaga taaattcatc tttttaatcc ttgaagcagc atcttcataa ctttcgcctg
     1501 gtcttgccaa tttttctata tcagctttta aaactccaaa gattgtttct ggagcagaag
     1561 catcaatatt atttttgagt tttaaagatt ttgctttttt aggaactact gatttcttta
     1621 actcaaacgc aaaaatcaaa tgactaacca ctctccctgt tttgcgttga gtataagtaa
     1681 cattcatatc cgaatgttgg ttgatttgag caactgcaac atcaataaca tactttttta
     1741 aatctttaat tgcaggatac ttatcttcaa tttggaatcg ttgcttgagc cattcaattt
     1801 ctatttccct ttttcctaca ctgccccatt gaatgagtaa ttcatataaa cgaacagcat
     1861 agatgctcgt catctttcca actacttcta atttatatcg tgtaaattgt gtttctaata
     1921 gcgataaaaa tggcaataca tcttgagaga aacgcaaacg aatacaacca tgttcaggca
     1981 agtatgcaat agagctaacc catctcgtta ataacgtttt aacacgaggg tcatttggat
     2041 aaggattttc taaagtaaca tgacggttaa ataatgtttt cgatacatca atcagctcac
     2101 tataagaagt ttttggatca acaccaaaat aagacactaa gtcactcgcg tgtacttcaa
     2161 acacatcatc taattgcagc tcacgatcac gtctgacacg agaaattgcc aaaagaatga
     2221 tacgctgctc atttaatgtt aagcgataac ttgcctctat taatcgattc gactttacaa
     2281 tcagttggtt attagaatta tcattcatag caatttaagg aacaaaaaaa aacactcctt
     2341 taataccgta aatccctccc tttatcaacc gtaaattact cctttaatac cgtattttcc
     2401 tcccttaata ccgtaaatcc ctccctttat catgttacaa gcaattgaaa ataaaaataa
     2461 aatcgacacg gaaaaggtat taaaagtatt taaaaggtat aaaaaagaaa ttttttgaaa
     2521 aatttataaa agatgatgat atctattgga ttagatatca tcattaaaat taatactcct
     2581 ctggcaacaa caacgttgtc accgacctat cggcctcggt aataatccac acgttgcctg
     2641 tttccaatgg gtaaaccgac ataatacgat agccacgttc aattgcttgt tgattgctta
     2701 acgcatcttc ggcaggcaca ttgccccaat cacctgattg atgacgagcc aataagctaa
     2761 agcaagaagt attgtgttga gctaacaggt caagggcatt gggtgtggcc accgtttgac
     2821 cgagtgaaaa taaaggattg ctgcacagta acatgatgat tctccgtgta ggttaaagtt
     2881 aattagttga agtgctttgt gcttgtaaac gctgaccgag cttttgcttt aaaacgccat
     2941 tggcggtttt aggggtagag cttgtattgt ttggattgtt tgaagtcgat tgaccttgtg
     3001 ccgagttatc atcatcagga ttgggataaa actcctccaa caactcagga atttcttctt
     3061 cgctaaattc aaattgtgcg ttgttaaatc cttcttctgc cgcttggtct aaggcctgtt
     3121 gctgaaagcc gatttctagc cgttgttggt ttagcgtttt ggcctgttgc aaggcttcgg
     3181 ctaaagtgtg gttatcacgt agtgttaaat ccacgtaata aatcgctgag cgatgacttt
     3241 gggtggtact tttacctc
//
LOCUS       DAOQMB010000259         3241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16112_length_3241_cov_13.315756, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000259 DAOQMB010000000
VERSION     DAOQMB010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16112_length_3241_cov_13.315756"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..857
                     /gene="ubiE"
                     /locus_tag="PLY05_08485"
     CDS             105..857
                     /gene="ubiE"
                     /locus_tag="PLY05_08485"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HNC03375.1"
                     /translation="MTNAKDNSTTHFGYQTVKTEEKVQKVAEVFHSVAAKYDLMNDLM
                     SMGIHRLWKRFTITLSGVRRGQQVLDIAGGTGDLARVFAREVGANGRVVLADINDSML
                     KVGRDKLIDGGYTGVEYVQANAECLPFADNTFDIITIAFGLRNVTDKDAALRSMYRVL
                     KPNGRLLVLEFSKPIYEPLSKVYDAYSFSVLPAMGQLITQDADSYRYLAESIRMHPDQ
                     NTLKEMMQNAGFISCDYHNLTGGIVALHRGYK"
     gene            857..>1459
                     /locus_tag="PLY05_08490"
     CDS             857..>1459
                     /locus_tag="PLY05_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HNC03376.1"
                     /translation="MLLPTVIALAAFEDMLNKALDLDPATRLQLNAQTGKSLLLNIQY
                     PDLSILVFFDDGKVRLTPAEDHLSLEPTATVTATSFDYIKQAFNKDQMITQSGLQLEG
                     DIFFLQTLQQIGLALDIDWEFGLSQLIGDIPAQQIGQGIRSLFSFAKQAAQSFLTHGG
                     EFLREESQLLPRKWQVDDFIEEVQELRTDIERLEARIALLK"
     assembly_gap    1460..1559
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1560..3184
                     /locus_tag="PLY05_08495"
     CDS             <1560..3184
                     /locus_tag="PLY05_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004932515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HNC03377.1"
                     /translation="PRLLEIWRICAAYRLDTLIPADAPVPLPVKLGLFILRIHPAWWS
                     NPVLTTYDDGERLRLALQELGVLFIKLGQLLSTRADLLPPNWIAELSKLQDRVPPFES
                     LLATQIVEQQLNAPLADIFSSFEQTPLAAASIAQVHTATLVDGREVIVKIVRPNLEQA
                     ISQDFAILKTASLWLEERTPAAAALHLHHVVCDYEQVLLSEIDLLQEAANTTRMRNNF
                     PNSSLIYIPEVHHKWCRSQVMVAERIYGVPISDKKTFIEKGVNLQVLAEKGLTIFFTQ
                     VFNHNFFHADMHPGNIFVDISKPENPKYIALDCAIMGELSDTDHLTVARLMMAVMQHD
                     FQQLINIAAQAGWIPPSTDTPALTREMRRLLSPMLQKSIAELDFAPILMDILEVARRY
                     HLEIPPQLVLLLKTLVHVEGLGRELYPDLDIWSLGKPLLAQWLSTRLNPVESMKKLGQ
                     QTPAMVLGLAELPNLIYDSLRGLKQNNAWQEKQWRELQNLHYQLAQSRRQDWLAFLAL
                     LTGAAGVSASDGLFALICAGLVVIAVIWRVLV"
BASE COUNT          879 a          652 c          650 g          960 t
ORIGIN      
        1 ataaaaaggc gcatcatgcg cctttgtttt ttgtcacaaa acttgattat ccctgtaatt
       61 catccgacaa tcagcacatc tttttgtcca ctcagttaaa gaccatgact aacgccaaag
      121 acaactctac tactcacttc ggctatcaaa ccgttaaaac agaagaaaaa gtccaaaaag
      181 ttgctgaagt attccattca gttgctgcca aatacgattt aatgaacgat ttgatgtcaa
      241 tgggtattca tcgtttgtgg aaacgtttta ccattacctt gtctggtgtg cgtcgtggtc
      301 agcaagtgct tgatattgca ggtggcacag gtgatttagc gcgtgttttt gcccgtgaag
      361 tgggtgcaaa tggtcgagtg gtgttagccg atattaacga ctccatgctc aaagttggcc
      421 gcgacaaatt aattgatggc ggttatacag gcgtggagta tgtgcaagct aatgctgagt
      481 gcttgccttt tgccgacaac acttttgata ttattaccat tgcctttggt ttgcgtaatg
      541 tcaccgacaa agatgctgcc ttacgttcta tgtatcgggt gttaaagccc aatggccgtt
      601 tattagtgtt agagttttct aagccaattt atgagccttt atccaaagtt tatgatgctt
      661 attcgtttag tgtgttacct gcaatggggc aactcatcac ccaagatgcg gatagttatc
      721 gttatttagc cgaatctatt cggatgcatc ccgaccaaaa caccctaaaa gaaatgatgc
      781 aaaatgcagg ttttatcagt tgtgattacc acaatttaac aggtggtatt gtggctttac
      841 atcgaggcta taaataatgt tattaccgac cgtcatcgcc ttggctgctt ttgaggatat
      901 gctcaataaa gccttagact tagaccctgc cactcgttta caactaaatg cacaaacagg
      961 caaaagttta ttattaaata ttcagtaccc cgacttgagt attttggtgt tttttgatga
     1021 tggtaaagtc cgtttaaccc ctgccgaaga ccacttaagc ttagagccaa ccgccactgt
     1081 aaccgccacg agttttgatt acatcaaaca agcgtttaat aaagatcaaa tgattaccca
     1141 aagtggttta caacttgaag gtgatatttt ctttttacag accttacaac agattggttt
     1201 ggccttagac attgattggg agtttggcct aagccaattg attggcgata ttcctgccca
     1261 acaaattggc caaggtattc gcagcttatt tagctttgcc aaacaagcag cacaaagctt
     1321 tttaacacat ggtggtgagt ttttacgcga agaatcacaa ctgttaccac gcaaatggca
     1381 agttgatgac tttattgaag aagtacaaga acttcgcacc gatattgagc gtctagaagc
     1441 acgtattgcg ttattaaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     1561 gccccgttta ttagaaatct ggcgtatttg tgcggcctat cgtttagata ccctgattcc
     1621 cgcagatgcg cctgttccac tgcctgttaa attaggttta ttcattttac gtattcaccc
     1681 tgcatggtgg tctaaccctg tgttaaccac ctatgatgat ggtgagcgtt tgcgcttagc
     1741 cctacaagag ctaggtgtgt tatttattaa actcggtcag ttattatcca cacgcgccga
     1801 tttattaccg cctaattgga ttgcagaact cagtaaacta caagatcgag tccctccctt
     1861 tgagagtttg ttagccacac aaattgttga gcaacaacta aatgcaccac tggcagatat
     1921 ttttagctct tttgaacaaa caccgttagc cgccgcctcc attgcccaag tgcacaccgc
     1981 caccttagtc gatggtcgtg aggtgattgt taaaattgtt cgccccaact tagaacaagc
     2041 gattagccaa gattttgcta ttttaaaaac cgcttccttg tggttagaag aacgcacacc
     2101 tgccgccgcc gccctacatt tacatcatgt ggtttgtgat tacgaacaag ttttattaag
     2161 tgaaatagac ttgctccaag aagccgcaaa caccacacga atgagaaata actttccaaa
     2221 ctcttcgttg atttatatac ccgaagtgca tcataaatgg tgtcgttcgc aagtgatggt
     2281 ggctgaacgt atttatggtg taccgatttc ggacaaaaaa accttcattg aaaaaggcgt
     2341 taatttacaa gtcttagccg aaaaaggctt aacgatattt tttacgcagg tgtttaatca
     2401 caactttttt catgctgata tgcaccctgg taatattttt gttgatattt ctaagcccga
     2461 aaatcctaaa tatattgcct tagattgtgc gattatgggt gaattaagcg acacagacca
     2521 tttaacggtt gcacgcttaa tgatggccgt aatgcaacat gacttccaac aattgattaa
     2581 tattgctgct caagcgggct ggattccacc gtcaacagat acaccagctc ttacccgcga
     2641 gatgcgccgt ctgttaagcc ccatgttaca aaaatcgatt gctgaattgg attttgcacc
     2701 gattttaatg gatattttag aagttgcacg acgctatcat ttggaaatcc cgccccaatt
     2761 agtcttattg ctaaaaacat tagtccatgt tgaaggcttg ggtcgagagc tttatcctga
     2821 tttagatatt tggtcattag gcaagccatt attggctcaa tggttaagca ctcgcctaaa
     2881 ccctgttgag tcaatgaaaa aactcggcca acaaacccct gctatggtct taggtttagc
     2941 cgaattgccc aatttgattt atgatagttt gcgtggttta aaacaaaata acgcatggca
     3001 agaaaaacaa tggcgtgagc tgcaaaactt acactatcaa ttagcccaaa gtcgtcgcca
     3061 agactggtta gcctttttag cactattaac gggtgcagcg ggggtgagtg ctagtgatgg
     3121 attgtttgcg ctaatttgtg caggtttagt ggttatcgcc gtcatttggc gagtattggt
     3181 ataaattatg agttttttag agacagtaaa atttgatgag caaggtttga ttcctgccat
     3241 t
//
LOCUS       DAOQMB010000260         3234 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16184_length_3234_cov_4.539792, whole genome shotgun sequence.
ACCESSION   DAOQMB010000260 DAOQMB010000000
VERSION     DAOQMB010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16184_length_3234_cov_4.539792"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="PLY05_08500"
     CDS             complement(<1..427)
                     /locus_tag="PLY05_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03378.1"
                     /translation="MSYAKWKNQNQSIATATVATVATLEQKIGFESDKKLSSVAKVAS
                     VAVAASENKKKSEPLPKIEPLSKSPVFGGGNSVILANGANQQTANGPVKKETYSPLED
                     GQLNRKSEDKQSPKNLGDVFKTEQTTAPNIRDTFTQIEET"
     gene            complement(430..3069)
                     /locus_tag="PLY05_08505"
     CDS             complement(430..3069)
                     /locus_tag="PLY05_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNC03379.1"
                     /translation="MFNRDLLPTPHAYYGQQFTLKGNKTKHLVPCCFHHDKTASLSLD
                     MVSGRFNCFGCGASGGDIVDFHQLKTGLSFVEAAKDLGAWVDTSTDTPEQLAARKAKI
                     EQANKEYKARQAQAAKDETSQAQAYIPLVAAILKQCTKPMLEPPYCVIKRINSHNLTT
                     TSATDIQRFTLPKDPSEPTAKPITVCYGFNGLLTVAILETLDGEPVALQVFDGVANNK
                     GKYARWIIGKPVVMGAYYHLGDFTAPSLVLITEGIADAISCYEATGYPCLAAISKGQL
                     ANAAQAVKSKYPQALIVLCGDNDADGGGEVEANKAAHAVNGVAVIPKGLESVKDFNDL
                     HQQHGLEAVKKMINEAIQTKKAVPPSQDGTAPLNDDLARESIDDVGNGGDGQGDEWQE
                     PEPIIAPQEMADYPIEHLPEPLRLLVLEIVDYLQCPIAMAANAVLAALSLSIQGLVNV
                     ARDNELIGAVSLFLMVIAESGERKSACDSLVTSHIKELDKQRLLADLDVLKFWQSDMD
                     TWTAEREGLISAIKQTAKNGDSTHSLKEQLKQLDEEKPKKPRGKTMLYEDATIEGLTK
                     GLFNNCPSGGVFSSEAGVVFGSHGMNSDNSMRNMATLNKLWDGGDVITNRADITKNML
                     LTGRRLTLSLATQESTVRAFFDSSKGLARGTGFGARFLIAWPKSTQGTRLYKAPPLSW
                     PNREAFVKRTTELLNTILTIDEESGALQPITLYLSPQAHDLWVEFYNYTEQELRIGGE
                     LTDIKDVTSKAADNVARIAALFHVYEHGLTGHISEAHILSANYLMDWYLMEAKRFFSE
                     AVLSKELRNTVLLDSWLIQHCQEQQTNHISTRDIQRKCPNSLRDKDLIDAILKHLADL
                     NRVKIVNQGKKKLIALNPKLLGV"
BASE COUNT          807 a          722 c          686 g         1019 t
ORIGIN      
        1 aggtttcctc aatttgagta aacgtgtccc gaatgtttgg ggcggtggtc tgctcagttt
       61 tgaatacatc tcctaaattt ttaggcgatt gcttatcctc acttttgcgg tttagctgtc
      121 cgtcctccaa tggtgaatag gtttcttttt taacagggcc atttgctgtt tgctgattag
      181 caccattggc caaaatgaca gaattaccgc caccaaaaac agggctttta gataaaggct
      241 ctattttggg gagtggctct gattttttct tattttcact tgctgcgact gcgacacttg
      301 cgacttttgc gacagatgat agttttttat cactttcaaa tcctattttt tgctcaagtg
      361 tcgcaactgt cgctactgtc gcggtcgcaa tagattgatt ttgatttttc catttagcgt
      421 aagacacgat tacaccccca ataacttagg gttaagtgca atcaatttct ttttaccttg
      481 attgactatt ttgacgcgat tcaagtctgc taaatgcttc aaaatggcat caattaaatc
      541 tttgtctcgt aaagagtttg ggcattttcg ctgaatatcc ctagtgctaa tgtggttcgt
      601 ttgctgttct tggcaatgtt gaataagcca actatccagt aataccgtgt tgcgtaattc
      661 tttggataat accgcctcac taaaaaagcg tttagcctcc atcaagtacc aatccattaa
      721 ataattagca cttaaaatat gcgcctccga aatatggcca gttaagccat gttcgtacac
      781 atgaaacaag gcggctattc ttgccacgtt atcggctgct ttactggtta cgtctttaat
      841 gtcggttagt tcgccaccta tgcgtaattc ctgctcggta tagttataaa actccaccca
      901 taaatcatgt gcctgtggtg ataaatacaa ggtaataggc tgtaatgcgc ctgattcttc
      961 atcaatggtt aaaatcgtat tgagtaactc ggtagtacgt ttaacaaacg cctcccgatt
     1021 aggccatgac aaaggcgggg ctttgtataa gcgtgtccct tgtgtcgatt ttggccaagc
     1081 gattaggaat ctagccccga atcctgtacc acgcgctaat cctttactac tgtcaaaaaa
     1141 tgcgcgtacc gtgctttctt gcgtggctaa agacaaggtt aaacgtctgc cagtgagtag
     1201 catattcttg gtaatatcgg ctcggttagt aatcacatcg ccaccgtccc aaagtttatt
     1261 aagtgtggcc atgttacgca tagagttatc gctattcatg ccatgactac caaaaacgac
     1321 acctgcctca ctagagaaca cgccaccact agggcaattg ttaaataagc ctttggttaa
     1381 gccctcaatg gttgcatctt catacaacat ggttttgccg cgtggttttt taggcttttc
     1441 ttcgtcaagc tgtttaagtt gctctttgag cgagtgggta gaatcaccat ttttagcggt
     1501 ttgtttaatg gcactaatta aaccctcacg ctcggctgtc catgtgtcca tatccgattg
     1561 ccaaaatttg agtacgtcta aatctgctaa taaacgttgt ttatccagtt ctttaatatg
     1621 actggtgact aagctatcac acgcactttt acgctcgcct gattcagcaa tcaccattaa
     1681 aaacaaagat actgcaccga ttaactcgtt atctcttgcc acattaacca agccttgaat
     1741 acttaacgat aaggcggcaa gcacggcatt ggctgccatt gctatagggc attgcaagta
     1801 atcgactatc tcaagcacta ataaacgtaa gggttcgggc aagtgttcta tggggtaatc
     1861 tgccatttct tgaggggcaa taatcggttc aggttcttgc cattcatcgc cttgaccatc
     1921 gccaccattg cccacatcgt ctatactttc ccttgctaaa tcgtcattta aaggggctgt
     1981 accatcttgg ctagggggta cggccttttt tgtttgtatc gcttcattaa tcattttttt
     2041 aaccgcctct aagccgtgtt gttggtgtaa atcattaaag tcttttacac tctccaaacc
     2101 tttaggaata accgctacac cattcaccgc gtgggctgct ttgttggctt caacttcacc
     2161 gccaccgtct gcatcgttat ccccacaaag tacaatcaag gcttgagggt atttgctttt
     2221 aacggcctgt gctgcattag ctaattgccc tttactaatt gcagctaaac aggggtagcc
     2281 tgtcgcctca tagcagctaa tggcatcagc tataccctca gtaattagca ctaggctagg
     2341 ggcagtaaaa tcacctaaat gataataagc acccatgacg acaggcttgc caataatcca
     2401 ccgtgcatat ttccctttgt tattggctac accgtcaaaa acctgtaggg caactggttc
     2461 accgtctagc gtttccaaaa tggcaaccgt taataagcca ttaaaaccat aacaaaccgt
     2521 gataggttta gccgttggtt cgctagggtc tttaggtaag gtaaaccgtt gtatatcagt
     2581 agcagaggta gtggttaagt tatggctgtt aatgcgttta atcacacaat aaggcggctc
     2641 tagcataggt ttggtgcatt gcttgaggat tgcagccact aaaggaatat aggcttgtgc
     2701 ttggcttgtt tcgtctttgg cggcctgtgc ttgacgtgcc ttatattcct tattggcttg
     2761 ctcaattttg gcttttcggg cggctaattg ttcgggtgtg tcggtagagg tgtccaccca
     2821 tgcccctaag tctttggcgg cttctacaaa actcaatcca gtctttaact ggtgaaagtc
     2881 cactatatcg ccaccacttg caccacaacc aaaacagtta aaacggcctg ataccatatc
     2941 taggctaagg cttgcggttt tgtcatggtg aaaacaacaa gggactaaat gcttagtttt
     3001 attgccttta agggtgaatt gttggccata gtaggcgtga ggggtaggga gtaaatcacg
     3061 gttaaacatg atatgcctcc ttgtcatttt cgcttgtatc agtgtttaag gtggcggtag
     3121 ctgcaatcgt attgtctgct gcttgcgctg cactatgacg cgctttaatg cgttgttgac
     3181 tttgattaaa tagctctaag ttgtgggctt tggcatcatt ggcaacaatg gcgc
//
LOCUS       DAOQMB010000261         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16262_length_3224_cov_6.275797, whole genome shotgun sequence.
ACCESSION   DAOQMB010000261 DAOQMB010000000
VERSION     DAOQMB010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16262_length_3224_cov_6.275797"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PLY05_08510"
     CDS             complement(<1..953)
                     /locus_tag="PLY05_08510"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HNC03380.1"
                     /translation="MSRIMLIIATGQNAANIQPLSETSLKPDAVCIAVTQTMMPQFND
                     LKNQITHLGIRVEEPLLLTNELDTAQLEQTFSQWLLEKHTDNEVLVNLAGGLKTITIA
                     AYKVFSALGAKIFYTHFDGQIIWVDAPLDTSLRIKPNISLAAYLKIYQYEITKKQNLA
                     DISKGHKQYVQGLSNYLRHNFDMRCHAISHLNWLAKPIIDSINKHERVSSKNHPKQSL
                     PTLFIENPKFNSQLDELYQAIPYIEEVFNISPKRLSFSSVESAKIVAGAWLDILVAET
                     LRQLPELNFRDICINVSFSKSTQRQGKVTFNELDVMALYGT"
     gene            complement(999..2921)
                     /gene="rmuC"
                     /locus_tag="PLY05_08515"
     CDS             complement(999..2921)
                     /gene="rmuC"
                     /locus_tag="PLY05_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNC03381.1"
                     /translation="MNTLIFISAFVACVVFLVIGIVIGRSLSKTMLLSLESQLKTQFD
                     AEKQKQVLEIQSFQQKLALKEQQNEQLEQFISNLKQDLQKNLTSLDNVRNQQAELNAK
                     LSASLSTEQGLTIRLQERDSIIAALNQEVVTLGKDKISQQELIEQQKVQLGQAQEHQQ
                     QLVLLRNEQQSKEQNLQATQQELTKAHAHIAELEMANQKDEKLIAEREKTIHQMQLSY
                     DTLSMQYEQHNQTIKQQNALIGQAQAEKEQLEQLQQQLQRKDESIQRLQNEATSYIST
                     IKELKTAAEKDAKNMAEKLALLEDNKSQLKQEFENLANKIFDNKQQHFATVSEHSLNS
                     LLNPFKEQLAAFRQRVDEVHSNNIEGQTTIKTELEKLRELNTRINDEAANLTKALKGD
                     KKLQGSWGEQKVDLLLQRSGLRKDIEYSKEENFKNEDGDNQRPDFVVKLPEGKHLIID
                     SKMSLVAYTQYVSADNEQARKQALEQHIKAVKEHINTLSQRKYHSLNGLNTPDFVFLF
                     MPIEPAYILIAEHAPEIFEQAYNKNIAIVTAANLLPVLRVVANLWSIQRQNQTTKELA
                     DQASRVYDKLRIFVEKMEDIGTHLNKAQDCYRDSFNSLRDGKGSLTKIVSNFVELGVK
                     VNKQLPASVTMEDLDN"
     gene            complement(3044..>3224)
                     /locus_tag="PLY05_08520"
     CDS             complement(3044..>3224)
                     /locus_tag="PLY05_08520"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HNC03382.1"
                     /translation="IYTALAYFILPVDLIPDFLPMGYTDDFGVLITAVSTVSMYITDE
                     VKQKTSATLKQWFGS"
BASE COUNT          918 a          546 c          588 g         1172 t
ORIGIN      
        1 ttagtaccat ataaagccat aacatctaat tcattaaagg taactttgcc ttgacgttgg
       61 gtgcttttgc taaatgagac attaatgcaa atgtctctaa aatttaactc agggagttga
      121 cgtaatgttt ctgcaactaa aatatctagc catgcgccag caactatttt tgctgattct
      181 acagagctaa agctaagacg tttaggactt atattaaaaa cttcttcgat ataagggatg
      241 gcttgatata actcatcaag ttgagaatta aattttggat tttcaataaa taaagttggc
      301 agactttgtt taggatgatt tttagatgag actcgctcat gcttgttgat gctatcaata
      361 atgggtttgg ctagccaatt taagtggcta attgcatgac atctcatatc aaagttgtga
      421 cgcaaataat ttgacaatcc ttgaacgtat tgtttgtgtc ctttagaaat atcagcaaga
      481 ttttgttttt tagtaatttc atactgataa attttaagat aggctgccaa actaatatta
      541 ggcttaatcc gtaatgatgt gtctaaaggt gcatctaccc aaataatttg accatcaaaa
      601 tgtgtataaa atatttttgc gcctaaagcc gaaaatacct tgtatgcagc aatggtaatg
      661 gtttttaaac ctcctgctaa attaactaag acttcattat ctgtatgttt ttctaacagc
      721 cattggctaa atgtttgttc taattgcgca gtatcaagct cattggttaa taataaaggt
      781 tcttcaacac gaatgcccaa atgagtaatt tgatttttca aatcattaaa ttgtggcatc
      841 atggtttgtg tgactgcaat acaaactgca tcaggtttta agctagtttc gcttaatggc
      901 tgaatgtttg ccgcgttttg tcctgttgca ataattaaca taatacgaga cataagattt
      961 tcctataaaa tagattttta aaaaagaggg gtatttattt agttatccaa atcttccatc
     1021 gtcacacttg ctggtaattg tttattaact ttaacgccta gctcaacaaa gttactaacg
     1081 atttttgtaa ggctaccttt accatctctt aggctattaa atgagtcgcg ataacagtct
     1141 tgagctttat taagatgtgt gccaatgtct tccatttttt caacaaaaat gcgtaactta
     1201 tcatacacac ggcttgcttg atcggccaat tctttagtgg tctgattttg acgttgaata
     1261 ctccataagt ttgcaaccac acgcaacaca ggtaataagt ttgctgctgt tacaatggct
     1321 atatttttgt tgtaggcttg ctcaaaaatc tctggcgcat gctcagcaat taaaatatat
     1381 gcaggctcaa tgggcataaa caaaaacaca aaatcgggcg tgtttaaacc attcagtgag
     1441 tgatatttac gttggctaag tgtgttaatg tgttccttaa cggctttgat gtgttgttct
     1501 aaagcctgtt tacgcgcttg ttcgttgtcg gcagacacat attgcgtata agccactaaa
     1561 gacattttgc tatcaataat caaatgtttg ccttcgggaa gttttactac aaagtctggg
     1621 cgttgattat cgccgtcttc atttttaaag ttttcctctt tgctgtattc aatatctttg
     1681 cgcaagccag aacgttgtaa taacaaatcg actttttgct caccccaaga gccttgtagc
     1741 ttcttatcac cttttaaggc tttggttaag ttagcggcct cgtcattaat acgagtattt
     1801 aattcacgta gcttttctag ctctgtttta atagtagttt ggccttcaat attattgcta
     1861 tgcacctcgt caacacgttg gcgaaaagct gctagttgct ctttgaatgg attaagtaaa
     1921 ctatttagac tgtgttcgct tactgtcgca aagtgttgtt gtttgttgtc aaaaatttta
     1981 ttagcaagat tttcaaattc ttgtttaagt tgagatttat tatcttctag caaagcgagt
     2041 ttttcggcca tatttttggc atctttttct gcagcagttt ttaactcttt gatggtgcta
     2101 atgtacgatg tggcttcgtt ttgtaaacgt tgaatagatt catctttgcg ttgtagttgt
     2161 tgctgtaatt gttcaagttg ttctttttcg gcttgagctt ggccaatcaa ggcattttgt
     2221 tgtttgatgg tttggttatg ttgttcatat tgcatagata aagtgtcata actcaattgc
     2281 atttgatgga tagttttttc acgttcggca ataagttttt catctttttg gtttgccatt
     2341 tctaattcgg caatatgtgc atgcgcttta gttaattctt gttgtgtggc ttgtagattt
     2401 tgttctttgc tttgttgttc attgcgtaat aatactaatt gttgttgatg ttcttgcgct
     2461 tggccaagtt gaactttttg ttgctcaatc aactcttgtt ggctgatttt gtctttgcct
     2521 aatgtcacga cttcttggtt gagagcagca atgatactat ctcgttcttg taaacgaatt
     2581 gtcaaacctt gctctgtaga aaggctagca ctgagttttg catttaactc cgcttgttga
     2641 tttctaacat tgtctaaaga cgttaagttt ttttgcaaat cctgtttaag attgctaata
     2701 aattgttcaa gctgctcatt ttgctgttct tttaaagcca atttttgctg aaaagattgt
     2761 atttctaata cttgtttttg cttctcggca tcaaactggg tttttaactg gctttctaag
     2821 ctcaataaca tggttttaga taaactacgt ccaatcacta taccaataac caaaaaaaca
     2881 acacaagcta caaaagcaga aataaaaatg agcgtattca tcgcgagtca ccttaaacaa
     2941 tcaaaaagtt aaaatcaatt atgtgtgata acaaatggca atttataaat tgcaagctta
     3001 tggaaagatt actaacagac tcatgtgcta atataagtcg tgttcaactt ccaaaccatt
     3061 gctttaaggt tgcacttgtt ttttgtttta cctcgtcagt gatgtacata gacacagtgc
     3121 taacagctgt aataagaacg ccaaaatcat cggtataacc cataggcaaa aaatcgggaa
     3181 ttaaatcaac aggcaaaata aaataggcga gggcggtgta aatg
//
LOCUS       DAOQMB010000262         3218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16317_length_3218_cov_14.583307, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000262 DAOQMB010000000
VERSION     DAOQMB010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16317_length_3218_cov_14.583307"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="PLY05_08525"
     CDS             <1..852
                     /locus_tag="PLY05_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004656345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HNC03383.1"
                     /translation="SSFACAVPGIMATRSISDTRDRLTTIMVAPLMTCSARLPVYALL
                     IGAFIPAQTVWGVFNLQGLVLFALYFGGIFSALVVATIIKWLRKDKSEHALLLELPSY
                     RLPDLRNVSLGLYERAGIFIKRVGGIILALTILLWVLLTFPQAPADATMPAIDYSFAG
                     RIGHALAVIFEPIGFGWQICIALIPAMAAREVVVSALGTVYAIAGNEELITEQLGTLI
                     AHQWSLATALSLLVWFVFAPQCLATLATVKRETASWRTVAIMAVYLFGTAYLASFITY
                     RVVLALS"
     gene            849..1118
                     /locus_tag="PLY05_08530"
     CDS             849..1118
                     /locus_tag="PLY05_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03384.1"
                     /translation="MNELIQIAIVSVIVLWSVWLTIKRYLPHWLYAQQQQLADYCLQH
                     KWMWLAHSLQPAAPIKTGCGSGCSSCSQGCPTSQTTEQVVRFVNK"
     gene            1186..>1505
                     /locus_tag="PLY05_08535"
     CDS             1186..>1505
                     /locus_tag="PLY05_08535"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNC03385.1"
                     /translation="MSHITKVIAILVSGIASLALAFVAFVLFIIDPNDYKSEIYKVVK
                     DKTDMDLAINDRIEWQLWPDIGLKLGKTSLTDSAAQQNLVNIQQASVSVQVMPLLSKQ
                     IAIDS"
     assembly_gap    1506..1605
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1606..>3218
                     /locus_tag="PLY05_08540"
     CDS             <1606..>3218
                     /locus_tag="PLY05_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HNC03386.1"
                     /translation="TISLDQEAQRYTLKGLTARSHLQGTLLPAPMIIDLKSDIVADVK
                     QQQHQLDNLKLAVDYQDANLTAPAKLELAANINANLQQQLINIKALQLSSSYPQKGLK
                     APATAKIQGDITANLTAQLWQIPNLVLEASYPASNLAAPATIKLTANSEANLQQQLIK
                     LTALKVAASYPDASRPSPITADVNGAVTANLSSGAVDFSPLDLQAKLSDKSFKKEMPI
                     HLVAPISANWKQGKIALNGFDLNALAINTKGQLLISLPALASTAAVKPPVTQGMSITG
                     NISTSPFNLRQLMQDLGMAAPVTKNANTLKTISVNSQISGNEQSVFLKGLKLKLDNTN
                     LTGDVGVNDFKAPKINARLAVDSINVDDYLPPTDPKAKPSTGGLLPIELLKQQNLDVG
                     LTIGALTVMNYPIKQFQVTAQANQGLVQVGKLAGGIYSGTFNLPTTIDVRGKEPLLTV
                     QPNIQQIEIANLVQQFTKQDLFTGKANYQGKLQLTGNSVQAWLHSISGNSSLKFDNGV
                     LKGVNMMKLVTSEMGKYQALLPYVTGKNA"
BASE COUNT          936 a          567 c          653 g          962 t
ORIGIN      
        1 tccagttttg cttgtgctgt acctggtatt atggcgacgc gaagtattag tgatacccgt
       61 gaccgtttaa cgacgattat ggttgcgcca ctgatgacct gttctgcgcg tttacccgtc
      121 tatgctttat taattggtgc atttattcct gcacaaacag tatggggagt ttttaattta
      181 caggggcttg tgctatttgc cttatatttt ggtggtattt ttagtgcttt agtggttgct
      241 acgattatca aatggttacg taaagataaa agtgaacatg ctttattatt agaattaccc
      301 agttatcgtt tgcccgattt acgcaacgtg agtttaggtt tatacgaaag ggcaggtatt
      361 ttcattaaac gggtgggtgg cattatttta gcattaacca ttttattatg ggtgttatta
      421 accttccctc aagcacccgc agatgcgacc atgccagcca tagattatag ttttgcggga
      481 cgtattggcc atgctctagc agttattttt gagcccattg gttttggatg gcaaatttgt
      541 attgccctta ttcctgcaat ggccgcacgc gaagttgttg tatcggcttt gggaacagtt
      601 tatgccattg ctggtaatga agaattgatt acagaacaat taggtactct aattgcacat
      661 caatggtcat tagcaacagc attatcgtta ttggtatggt tcgtatttgc gccacaatgc
      721 ttggcaacat tagccactgt taagcgcgaa acagcttctt ggcgtactgt ggcaattatg
      781 gcagtgtatt tatttggtac agcctatctt gcatcattta tcacttatcg ggtggtgtta
      841 gccttatcat gaatgagttg attcagatcg caatagtgag cgtgattgtg ttgtggagtg
      901 tatggctaac cataaagcgt tatttacctc attggttgta tgcacaacaa cagcaattag
      961 cggattattg tttgcaacat aaatggatgt ggcttgctca tagcttacag cctgctgctc
     1021 ctataaaaac gggatgtggt agtggttgta gtagttgttc tcaaggctgt ccaacatcgc
     1081 aaacaacaga gcaagttgtc agatttgtta ataaataagc aataactcct tcttgttaaa
     1141 tatgctatct ttgccttgtt ttgaatttga caaggtgtct atacgatgtc acacattacc
     1201 aaagttatag ccattttagt gagtggtatt gctagcctcg ccttggcatt tgtcgccttt
     1261 gtgttgttta ttattgatcc taacgattat aaaagcgaaa tttataaggt tgtgaaagac
     1321 aaaaccgata tggatttggc cattaatgat cgtattgaat ggcaactgtg gccagacatt
     1381 ggtttaaagc ttggcaagac cagtttaacg gatagtgccg cacaacaaaa tctggtcaat
     1441 attcaacaag cgtctgtttc tgtgcaagtg atgccgttat taagcaaaca aatagcgatt
     1501 gattcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnacgat tagtctagat
     1621 caagaagcac aacgctatac gcttaaaggt ttaaccgcac gtagtcattt gcaaggcaca
     1681 ttattacccg ccccaatgat tattgattta aaatcggata ttgttgctga tgtaaaacaa
     1741 cagcaacatc aactcgataa tctaaagtta gcagtcgatt atcaagatgc gaatttaaca
     1801 gcaccagcca agctagagtt ggcagcgaat attaacgcaa atttgcaaca acaattaatt
     1861 aatatcaagg ctttacagtt aagcagtagt tatccacaaa aaggcttaaa agcccccgca
     1921 acggcaaaaa ttcagggcga tattacagca aatctaacag ctcaattatg gcagattcct
     1981 aatttagtcc tagaagctag ttatccagca agtaatttag cggcacctgc cacgattaaa
     2041 ttaaccgcca atagtgaagc caatttgcaa caacaactga ttaaattaac tgctttaaaa
     2101 gtcgcagcta gttatcctga tgcaagcaga cctagtccga ttacggcgga tgttaatggc
     2161 gcagtgacag caaatttaag ctcaggtgcg gttgattttt cacctttaga tttgcaagct
     2221 aaattgagcg ataaatcctt taagaaagaa atgcctatcc atttagtcgc accaatttct
     2281 gcaaattgga aacagggaaa aattgctctt aatggttttg atttaaatgc cttggcaatt
     2341 aataccaaag ggcagttatt gattagtttg ccagctttag ccagtacggc tgcagttaag
     2401 ccccctgtga ctcaagggat gagtattact ggcaacatca gcacttcgcc ctttaattta
     2461 cgccaattaa tgcaagattt aggtatggca gcccctgtca ctaaaaatgc taatacctta
     2521 aaaacaatca gtgttaacag tcaaattagt ggtaatgaac aaagtgtatt tttaaaaggt
     2581 ttaaagctaa aactggataa caccaattta acaggtgatg tgggtgttaa tgattttaaa
     2641 gctcccaaaa ttaatgctcg tttagcggtt gattcgatta atgttgatga ttatttgccc
     2701 cctacagatc ctaaagccaa acctagcaca ggcggtttgt tgcctataga gttattaaag
     2761 caacaaaatt tagatgttgg gctgaccatt ggtgcgttaa cggtgatgaa ttatcccatc
     2821 aaacaatttc aagtgaccgc acaagctaat caagggttag tgcaagttgg aaagttggca
     2881 ggtggtattt acagtggtac gtttaattta ccaacaacca ttgatgtacg tggtaaagag
     2941 ccattattaa ctgtacaacc caacattcaa caaattgaaa ttgccaattt agtgcagcaa
     3001 tttaccaaac aagacttatt tacaggtaaa gctaattatc aaggtaagtt acagttaaca
     3061 ggtaattctg tacaagcgtg gttacatagc attagcggta attcaagttt aaaatttgat
     3121 aatggcgtgt taaaaggcgt gaatatgatg aagctcgtta ccagcgaaat gggcaaatat
     3181 caagccttat tgccgtatgt gacaggtaaa aatgctga
//
LOCUS       DAOQMB010000263         3212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16367_length_3212_cov_15.917643, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000263 DAOQMB010000000
VERSION     DAOQMB010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16367_length_3212_cov_15.917643"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1578
                     /locus_tag="PLY05_08545"
     CDS             172..1578
                     /locus_tag="PLY05_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC03387.1"
                     /translation="MLHQLKLRSKLFLLISSANFILACLLYWFNAVSFSSGFSHYLGQ
                     QFLAQQQNLAIALVNHYQQHQGWQNLQAQPEVFFNFARQYAKHSNEHHPPPKPKEHFL
                     PPPPPNHYDDWFGLSILTPQKRNLFTGRPIPKEAALLPLYNQQQLIGYLSYLPQHEQS
                     QQLNQAFKQQQQQSFIISSVSLLVISILIAWLIAQWLNKRLLPLNQATKALSAGDYHY
                     RIPLQSEDELDELAKAINQLAADLAHSLHSRQQWIIDISHELRTPLTILSGELAALLE
                     GIRPLSMQQIESLWQEIKRLSRLVDDLHLLSQADAGSLSYHWQTLDLSECLEHVLFNV
                     DLEFAKKNIDIIFQPNPVFIEGDHQRLQQLWLNLAQNTLRYTDVNGALKIDIVSNNDS
                     VTVIWQDSAPSVPDADLQRLTERLFRVDSSRHRQTGGSGLGLSIVKAIADAHHASLTA
                     SHSELGGLCWHISFPKQP"
     gene            1583..2350
                     /locus_tag="PLY05_08550"
     CDS             1583..2350
                     /locus_tag="PLY05_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03388.1"
                     /translation="MQTAHILLVEDETKIAQLLQDYLQHEGFVCSLLDSGDLVEPFLA
                     THNCQLILLDIMLPAISGLELCKRIRQSSHIPIILLTARVEEIDRLLGLELGADDYIC
                     KPFSPREVVARVKAVLRRSTLPTSVTAPEKPPVLHLDKASFQAKLHGQDLALTAVEFS
                     LLAVLVEQPTRIFSREQLMNAMYQDHRVVSDRTIDSHIKKIRRKMASICPDQEFIHAL
                     YGVGFDILPTLNTSTKLSNLGRGFLLQDAHVRARECS"
     gene            complement(2286..>3212)
                     /locus_tag="PLY05_08555"
     CDS             complement(2286..>3212)
                     /locus_tag="PLY05_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004814062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HNC03389.1"
                     /translation="AFDIAKYTAGASKELNLHSQTIQAITEELVLRRKQFKKAKLNWR
                     VSNPTSPKKSLGWIPFKKTAIKYQHGQVKYGNHWFGLWDSYGLSTYNIKTGSFVQDSR
                     GCWYVCLVVDNAAKVASRGTESVGIDLGLKDFATLSNGEKIESQRTYRRYEQKLGIAQ
                     RARNKKRVKAIHAKIKQIRHNHLHQESTKLVKNYAAIFVGNVNAKSLAKTKLAKSVLD
                     AGWTLFRTLLKYKSENAGVWFDEVNEQYTTQTCSCCGSRDSSPKGRAGLGIREWTCVQ
                     CGTLHDRDVNAAKNILALGHERLAEGILALGY"
BASE COUNT          897 a          670 c          612 g         1033 t
ORIGIN      
        1 gctattgggg cgatgcacgc actcatggtc ggcaaatttt gccaaaaatt aatggcggtg
       61 atggctttta ttatgccttg ttagagaaaa aattttaaaa tccttttttc ttgcaccatt
      121 acgcaattaa ttaagcatac aatctgctct aaactctttt gagcagattg tatgttgcac
      181 caactaaaat tacgcagcaa actattttta ttaattagca gtgctaattt tatattagcc
      241 tgtttgttat attggtttaa tgcggtaagt tttagctcag gttttagcca ttatttaggc
      301 caacagtttt tagcacaaca gcaaaattta gcgattgctt tagtgaatca ttatcagcaa
      361 catcaaggct ggcaaaactt acaagctcag cccgaagtat tttttaattt tgcacgacaa
      421 tacgcaaaac attctaatga acatcatcct ccaccaaagc ctaaagagca ttttttacca
      481 ccaccgccac cgaatcatta tgatgattgg tttggtttaa gtattttaac gccacaaaaa
      541 cgtaatttgt ttacaggccg ccctattcct aaagaagcgg cattattacc tttatataat
      601 caacaacaat taattggcta tttaagttat ttacctcaac acgaacaaag ccaacaactc
      661 aatcaggctt ttaaacaaca acaacaacaa agctttatta ttagcagtgt gagtctgttg
      721 gtgatttcaa ttttgattgc atggctaatt gcccaatggc taaacaaacg ccttttaccg
      781 ttaaatcaag cgacaaaagc cctgagtgcg ggcgattatc actatcgtat tcctttgcaa
      841 agcgaagatg aattagatga actcgccaaa gcgattaatc aattagcggc tgatttagcg
      901 catagcttac atagtcgaca acaatggatt attgatattt cacatgaact gcgtacacct
      961 ttaaccattt tatctggcga attagcagca ttactggaag gcattcgtcc tttatccatg
     1021 cagcagatag aatcgttatg gcaagaaatt aaacgtttat cgcgcttagt tgacgactta
     1081 catttacttt ctcaagccga tgcaggctct ttaagttatc actggcaaac ccttgattta
     1141 agtgaatgtt tagaacatgt gttatttaat gtcgatttag agtttgctaa gaaaaatatt
     1201 gacattattt ttcaaccaaa tcctgtattc attgaaggcg atcatcaacg cttacaacaa
     1261 ttatggctaa acttggcaca aaacacctta cgttacaccg atgttaatgg cgcactcaaa
     1321 attgacattg tcagcaataa cgattcagtg actgtcattt ggcaagactc cgcacctagt
     1381 gtacctgatg ctgatttaca gcgtttaacc gaacggttat ttagagtgga tagttctcgt
     1441 catcgacaaa caggcggttc aggcttaggc ttatccattg ttaaagccat tgccgatgca
     1501 catcatgcaa gcttaacggc tagtcacagt gagttaggtg gcttatgttg gcacatttct
     1561 tttcctaagc aaccataaaa ttatgcaaac agctcatatc ttattagtcg aagatgaaac
     1621 caaaatcgca caactgctgc aagactatct acaacatgaa ggctttgttt gtagcttatt
     1681 agatagcggt gatttggttg agcctttttt agcaacgcat aattgccagt tgattttatt
     1741 agacattatg ctgcctgcta tttctggcct agaattatgc aaacgcatta ggcaatctag
     1801 ccatattccg attattttac tcactgctcg tgttgaagaa atagatcgtt tacttggttt
     1861 agagttaggt gcagatgact atatttgtaa gccctttagc ccgcgtgaag tggtggctcg
     1921 tgtgaaagcg gttttgcgtc gtagtacatt gccaaccagt gtcacagccc ccgaaaagcc
     1981 acctgtatta catctcgata aagccagttt tcaagccaag ttacatggtc aagatttggc
     2041 tctaaccgca gttgaattta gcctattagc cgttttagtt gaacagccaa cacgtatttt
     2101 tagccgcgaa caattaatga atgctatgta tcaagatcat cgcgtggttt ctgatcgcac
     2161 cattgatagc catatcaaaa aaattcgccg caaaatggca agcatttgcc cagaccaaga
     2221 atttattcat gccttatatg gtgttggttt tgacatcctc cccaccctca atacttcgac
     2281 taagctcagt aacctagggc gaggattcct tctgcaagac gctcatgtcc gagcgcgaga
     2341 atgttcttag cggcgtttac gtcgcggtca tgcagtgtac cacactgcac acaagtccat
     2401 tctcttattc caagtcctgc tctacctttc ggactactgt cgcgtgagcc acagcacgag
     2461 caagtttggg tggtatattg ttcattcact tcgtcaaacc atacccctgc gttctcgctt
     2521 ttgtacttga gtagggttct aaacagtgtc cagcctgcat ctaataccga ttttgctagc
     2581 ttagttttgg ctagactttt ggcattgacg ttgccaacaa atatcgcggc atagttttta
     2641 actaattttg tactttcttg gtgtaagtga ttatgtctaa tttgtttaat tttggcatgt
     2701 atcgctttaa cgcgtttttt gtttctcgca cgttgagcaa taccgagttt ttgttcgtat
     2761 cttcggtagg tgcgttggga ttctatcttt tctccattag acaaggtagc aaagtctttt
     2821 aaacctaagt ctataccaac agattctgtg cctcttgagg cgactttagc cgcattatca
     2881 acgaccaagc acacatacca acagccacga ctatcttgga caaatgagcc tgttttgatg
     2941 ttatatgttg acaatccgta actatcccac aaaccaaacc agtgattgcc atatttgact
     3001 tggccgtgtt gatatttaat cgctgtcttt ttgaagggta tccaaccaag tgatttttta
     3061 ggggatgtgg gattcgatac gcgccagttg agtttggctt ttttgaattg tttgcgtctt
     3121 aacactaatt cttcggtaat cgcttgtata gtttggctgt gcagatttaa ttcttttgat
     3181 gcgcctgctg tgtattttgc aatatcaaaa gc
//
LOCUS       DAOQMB010000264         3204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16435_length_3204_cov_6.462369, whole genome shotgun sequence.
ACCESSION   DAOQMB010000264 DAOQMB010000000
VERSION     DAOQMB010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_3204_cov_6.462369"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLY05_08560"
     CDS             complement(<1..198)
                     /locus_tag="PLY05_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03390.1"
                     /translation="MLLLRQILGRFNFPAMLAFLTVVAFTTLRLLLTVISWPQLDHNL
                     SELASVFLRGLLFDGVVAVYVY"
     gene            564..1103
                     /locus_tag="PLY05_08565"
     CDS             564..1103
                     /locus_tag="PLY05_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNC03391.1"
                     /translation="MKGIVFNLLEDVVTAHHDADTWDALLDKAGLSGAYTSLGSYPDK
                     EIERLVLATAEMLQISLDEVLCWFGQQAIPLLAQRYPVFFANHQSTRSFLLTLNNIIH
                     PEVKKMYKGAEVPTFDFNDNDEHLLVMGYYSARQLCPLAEGLTRGAAAYFKENIQFSH
                     TACMREGDLKCVFHVSFNP"
     gene            1116..3119
                     /locus_tag="PLY05_08570"
     CDS             1116..3119
                     /locus_tag="PLY05_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNC03392.1"
                     /translation="MTSAPNPEVEIARLKKRLERERQARLEAETIAEQGLRSLYEKQQ
                     ELELLESIAVAANQANSLHEILELVMSRICQFSHWPIAHAYLRDSHLSTRLYPSQTWY
                     RATRYNIQAFCELTEKTYVNYGEDVSGQAMATGESTWLELNHDCTLPRQQAALACGIK
                     TAFALPILAGKQVIAVIEFFHHLQLRADPKLSHLLSQVGLQLGRIADRQLAEARLLHD
                     AFHDPLTKLPNRAFFLDRLQRAIARRQRFGNYKFAVLFIDLDRFKVINDSLGHAAGDS
                     LIRHIAERLSTALQEKFSIASYSEFKSVLARAGSDEFVLLLEYIENPQDVLTVIARIH
                     QALANPFVIHEQALHVSISIGVASPDNQLSADDILRNADLAMYEAKRQGGGNVQFFDQ
                     TMHDKALKRLLVENDIRHALQQQQFVLFYQPIVSLQTGHIVGVEALIRWQKTPDKLIF
                     PNDFIFIAEESGLITQIDMWVLRQACQTVQSWQKRFPNQPLTVSVNLSARSFALPNIV
                     EIISHTLAETGVRPSAIRLEITESMTMNDTEKVIAMLVELREMGLHLSIDDFGTGYSS
                     LSYLHRFPADILKIDRSFVSRMDESYECLQIINSIMSLARNLNMKVIAEGPETEAHVQ
                     QLQELDCNFGQGYFFSRPISQDQLEALLTKQWSYNLPHIVNHS"
BASE COUNT          976 a          653 c          603 g          972 t
ORIGIN      
        1 atacacatag acggcaacaa caccatcaaa taataagcca cgtaaaaata cgctcgccaa
       61 ctctgaaaga ttatgatcta actgaggcca tgagatgact gtcaataaca ggcgtaaagt
      121 cgtaaatgct acaacagtta aaaaggctaa catggcgggg aaattaaaac gccctaaaat
      181 ctgtcggagc aataacatag aataccaact taagctttac aaaaaatcac cacatattta
      241 tgaagcacta aataacgctt aattaagagt tattaattaa agctactcta ttctattttt
      301 cttaaagaaa acttaatgat ttagtattta tcatgttaaa aataacctta atataattaa
      361 ggagataaaa aaatctagca aatacttgct aattaaaaaa gcaaaacgaa ttgcatactt
      421 atgattactt tgctatataa atgttacata taatttttta ataatgaatt tagccacatg
      481 aattggcact taaattgctg gtcttttttt cgatccagat ttaaatttta tacctatagc
      541 attgctgata tagggagttt tatatgaaag gtattgtctt taaccttctt gaagacgtgg
      601 tcactgctca ccatgatgcg gatacatggg atgcccttct agacaaagcg ggtttatcgg
      661 gcgcatatac ctcgttgggt agttatccag acaaagagat tgagcgatta gtgttggcta
      721 ccgcagagat gttgcaaatt tcacttgatg aagtgttatg ttggtttggt caacaagcaa
      781 ttcccttgtt agcacaacgt tatcctgtct tttttgccaa tcatcaatcg acacgttctt
      841 ttttattaac cttaaataat attattcatc ctgaagttaa aaagatgtac aaaggtgcag
      901 aagtacccac tttcgacttt aatgataatg atgaacattt attagtaatg ggctattact
      961 ctgctagaca attgtgccct cttgctgaag ggctaacacg tggtgcagct gcctatttta
     1021 aagaaaacat tcagttctcc catacagctt gtatgcgtga gggtgatctt aaatgtgtgt
     1081 ttcatgttag ttttaatcct taagagtagc aaagaatgac ttctgcacca aaccctgaag
     1141 tagaaatcgc tagactcaaa aaacgcttag agcgtgagcg ccaagcgcgt cttgaggccg
     1201 agactattgc tgagcagggt ttacgctctt tatatgaaaa acaacaagaa cttgaattat
     1261 tggaatctat agccgttgcc gctaaccaag ccaactcctt acatgaaatc cttgaattag
     1321 tcatgagtcg aatttgccaa tttagccatt ggccgattgc ccacgcctat ttacgggata
     1381 gccatttatc cactcgctta tacccctcac aaacatggta tcgtgccacg cgttataata
     1441 ttcaagcgtt ttgtgagtta accgaaaaaa cctatgttaa ctatggtgaa gatgtttctg
     1501 gacaagcaat ggctacggga gaaagcacat ggctagaact caatcatgat tgcacactgc
     1561 ctagacaaca agcagcgtta gcctgtggca ttaaaacggc ttttgcattg ccgattttgg
     1621 caggcaaaca agtgattgct gtgattgaat tttttcacca tttacaatta cgtgctgatc
     1681 ccaaactcag tcatttactc tctcaagtag gtttacaatt aggtcgaatt gccgatcgac
     1741 aactagccga agcacgttta ctgcatgatg cctttcatga ccctttaacc aaactcccca
     1801 atcgggcatt ctttttagat cgtctacaac gagcaattgc acgccgacag cgttttggca
     1861 actacaaatt tgccgtattg tttattgatc ttgatcgttt taaagtcatt aatgatagtt
     1921 tgggacatgc cgcaggtgat agtttaattc gtcatattgc tgagcgttta tcaactgcct
     1981 tacaagaaaa gttttctatt gcctcatata gcgaatttaa aagtgtgctt gcacgtgcgg
     2041 gtagtgatga gtttgtgttg ctcttagagt acattgaaaa tccacaagat gtgttaacgg
     2101 ttattgcacg tattcatcaa gctttagcca acccctttgt gattcacgaa caagcattac
     2161 atgttagtat tagtattggc gttgcttccc ccgacaatca attatctgcc gatgacattt
     2221 tgcgtaatgc tgacttagcc atgtacgaag ccaaacgcca aggtggaggt aatgttcaat
     2281 tttttgatca gaccatgcac gacaaagcac tgaagcgttt attggtcgaa aatgatattc
     2341 gccatgcctt acaacaacaa caatttgtgt tgttttatca gccgattgtc tccttacaaa
     2401 ctggacatat tgttggcgtt gaagccttaa tacgctggca aaaaactccc gacaaattga
     2461 ttttccccaa tgattttatt tttattgccg aagaaagtgg cttaattacc caaattgata
     2521 tgtgggtgtt acgccaagcc tgccaaacag tgcaatcatg gcaaaaacgc tttcccaacc
     2581 aacctctcac cgtgagtgtt aatttatctg ctcgctcttt tgctttgccc aatattgtcg
     2641 aaattattag tcatacctta gctgaaacag gtgttcgccc ctcagccatt cgcttagaaa
     2701 ttaccgaaag tatgaccatg aatgacaccg aaaaagtcat tgccatgttg gttgagctac
     2761 gtgaaatggg actacatttg agtattgatg actttggtac aggctattca tcattaagct
     2821 acttacatcg cttccctgcc gatattttaa aaattgaccg ctcttttgtc tcgcgtatgg
     2881 atgagagtta tgagtgcttg caaattatta actccatcat gagtttagct cgcaacctaa
     2941 acatgaaagt aattgcggaa ggccccgaaa ctgaagccca cgttcaacaa ttacaagagc
     3001 tagattgcaa ctttggtcaa ggctactttt tctctagacc cattagccaa gatcagcttg
     3061 aagccttatt aaccaaacaa tggagctaca acctccccca cattgtcaac catagttaac
     3121 tgtttgtggt gtgcaagttt aaagcagtgg tgtaaaacca ctggccttgc tctttgacaa
     3181 attgtgagcg ctcatgcaaa acat
//
LOCUS       DAOQMB010000265         3181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16616_length_3181_cov_14.207614, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000265 DAOQMB010000000
VERSION     DAOQMB010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16616_length_3181_cov_14.207614"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1223
                     /locus_tag="PLY05_08575"
     CDS             78..1223
                     /locus_tag="PLY05_08575"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC03393.1"
                     /translation="MTPKPTFSHLGIDSLLVKAVNELNYQQPTPIQQTAIPAILQGRD
                     VVAGAETGSGKTAAFALPLLQRLSQQAQTSQAKGNQIRALILAPTRELAIQIGQAINS
                     YGQYLQPRLKVCTVFGGVSINPQMLSLRGGADIVVATTGRLLDLMAHNALKCDALAYL
                     VLDEADKMLGLGFSEELTQILKALPKKRQNLLFSATFPPELQQLIEQLLHQPLEINLA
                     KQDENLIEQHVYVVEQERKNAALIHLIKSENWQQVLVFASAKNTCNRLVLKLAKAGLQ
                     AAAFHGDLSQSARNKALEDFKAKKIRVLIATDVAARGIDIQQLPYVVNFELPRSANDY
                     IHRIGRTGRAGQQGYAVALITEEDEDHFRLIEKRMKKRLPREQILGL"
     gene            1233..2177
                     /locus_tag="PLY05_08580"
     CDS             1233..2177
                     /locus_tag="PLY05_08580"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF003558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HNC03394.1"
                     /translation="MSVYTELTLSEIQEFVSKYQLQAIAYQPIQAGIENSNYFVSTAN
                     GQEFVLTIFEELNGQEANFLAPLLQHLQQAGVAVAAPLIANNGQSLLTIKDKAAQLAP
                     RIQGQHPLQVNVAQAQVIGEQLAKLHLALKKYPLKRSNAHGAAWWQKEAAKARNGMTQ
                     VEQLILDTVLNDFEETTEDFDDLPKGLIHGDLFRDNTLFAGDELKAILDFSEAGKDYW
                     LLDIAITINDFCSDWPNVTLNQELYQAFLTGYQKKRQLTEDEQEVLPTFLTMAATRFW
                     LSRLSVAKRNVAEGRTGEHVLQKDPQEMLAMVKARLAE"
     gene            2241..3080
                     /locus_tag="PLY05_08585"
     CDS             2241..3080
                     /locus_tag="PLY05_08585"
                     /inference="COORDINATES: protein motif:HMM:NF024899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HNC03395.1"
                     /translation="MVNIGQVNRLEVVEQLKQHYLLEGGRYGDIPIAKQDVPQGTKVG
                     QSIDAFVFIDEEGYLAASSQQPVAQVGQFASLKVLSVTPEGAWLDWGIEPPLFLSRKE
                     QQHVLEPNRYCLVRLCFEERKGIYATTYLNNFLQNECTEFKQGDKVSILIASPTELGF
                     RVIVNHRYWGALYANEVFQQVYKGQILEAYVKRLREDKRLDLSLQPLGFAKVTGITDD
                     ILQRLKQQKGYLMLSDKSSPDEIYAAFGVSKKVFKQAIGTLFKQRQIIIEEQGIRLAN
                     RKI"
BASE COUNT          988 a          585 c          682 g          926 t
ORIGIN      
        1 agtctaaaaa taaggcgagt aacataacag aattacgtta aaatagcggc tacttttaat
       61 ctttgaaacg attgttaatg acacctaagc ctacttttag ccatcttggt attgattctt
      121 tattagttaa agcagtcaat gagcttaatt accaacaacc cacccccatt caacaaaccg
      181 ctattcccgc aattttgcag ggacgtgatg tggtggcagg cgcagaaaca ggctcaggca
      241 aaaccgctgc gtttgcttta ccgttgttgc aacgtttatc tcagcaagca caaacaagtc
      301 aggcgaaagg caatcagatt cgcgccttaa ttcttgcccc aactcgtgaa ttagccatac
      361 aaataggtca ggccatcaat agttatggcc aatatttgca gccacgtctc aaggtatgta
      421 cggtgtttgg tggtgtctcc attaacccac aaatgttgtc tttgcgtggt ggtgcagata
      481 ttgttgtggc gacaacaggc cgtttgctcg atttaatggc gcataatgcg cttaaatgtg
      541 atgctttagc gtatttagtg ttggatgaag ccgacaagat gcttggatta ggctttagtg
      601 aagaattaac ccaaattctt aaagctttgc caaaaaaacg ccaaaacttg ttattttcgg
      661 caacatttcc acccgaatta caacagttga ttgagcaatt attacatcaa cctctcgaaa
      721 ttaaccttgc gaaacaagac gaaaatttaa ttgaacagca tgtatatgtc gttgagcaag
      781 aacgtaaaaa tgcggcttta attcatttaa ttaaaagtga aaattggcaa caagtattag
      841 tatttgccag tgctaaaaac acctgtaatc gtttggtgtt aaaattggcc aaagcaggac
      901 ttcaagccgc cgcgtttcat ggcgatttaa gtcaatcggc gcgcaataaa gctttagagg
      961 attttaaagc caaaaaaatt cgcgtgttga ttgcgacgga tgtggctgca cgaggtattg
     1021 atattcaaca attgccttat gtggtgaatt ttgaattgcc acgctcagcc aatgattata
     1081 ttcatcggat tggtcgcaca ggccgtgcag gtcaacaagg ttatgctgtg gcattaatta
     1141 ctgaagaaga tgaagatcat tttcgattaa ttgaaaaacg catgaaaaaa cgtttgccgc
     1201 gtgagcaaat tcttggactt taggagaaaa taatgtctgt ttataccgaa ttaaccttaa
     1261 gcgaaattca agagtttgtt agcaaatacc aattacaagc gattgcctat caacctattc
     1321 aagcaggaat agaaaacagt aattattttg ttagcactgc caatggccaa gaatttgtgc
     1381 taaccatttt tgaagagtta aatggccaag aggcaaattt tttagcgcct ttattacagc
     1441 atttacaaca agctggcgtg gctgtagccg ctccgttaat cgccaataat ggccaaagtt
     1501 tattgaccat taaagataaa gccgcgcaat tagcaccacg cattcagggt caacatccct
     1561 tacaggttaa cgttgctcaa gcacaagtta ttggtgagca actggctaaa ttgcatttag
     1621 ctctaaagaa atacccactc aaacgcagca atgcgcatgg ggcagcttgg tggcaaaaag
     1681 aagccgccaa agcacgcaat ggcatgactc aagttgaaca gttgatttta gatactgtgc
     1741 tcaatgattt tgaagaaacc accgaagatt ttgatgattt gcctaaaggc ttaattcatg
     1801 gtgatttatt tcgcgataac accttatttg ctggtgatga actcaaagct attttagatt
     1861 tttccgaagc gggtaaagat tattggttgc tagatattgc tattaccatc aatgattttt
     1921 gtagtgattg gccgaatgta acgcttaatc aagagttata tcaagccttt ttaactggct
     1981 atcaaaaaaa gcgccagtta accgaagatg agcaagaagt cttacccacg tttttaacca
     2041 tggcggcaac acgtttttgg ctatcacggc tcagtgttgc caaacgtaat gttgccgaag
     2101 gtcgcacagg tgaacatgtg ttacaaaaag acccgcaaga aatgttagcg atggttaagg
     2161 cacgtttggc agaatagggg ctgtcaaaaa atcagcaccc cctaaaccct gtagaaattg
     2221 taatttttta gagaaataac atggttaata ttggccaagt caatcgttta gaagtggttg
     2281 agcaactcaa gcagcattat ttattggaag ggggacgtta tggtgatatt ccgattgcta
     2341 aacaagatgt tcctcaaggg actaaggttg gccaatctat agatgctttt gtatttattg
     2401 acgaagaagg ttatttagcc gctagcagtc aacagcctgt tgctcaggtg ggacaatttg
     2461 cttccttgaa agtgttatca gtgacaccag aaggggcatg gcttgattgg ggcatagaac
     2521 cgcctttatt tttatcgcgt aaagaacagc agcacgttct agaaccaaat cgctattgtt
     2581 tggtacgttt gtgttttgaa gaacgtaaag gtatttatgc aacgacatat ttaaataact
     2641 ttttgcaaaa tgaatgtaca gaatttaaac aaggcgataa agtctccatt ttaattgcga
     2701 gtcccacaga gttaggtttt cgtgtgattg tcaatcatcg ttattggggg gcgttatatg
     2761 caaatgaagt gttccaacaa gtctataaag gtcaaatatt agaggcctat gttaagcgtt
     2821 tgcgcgaaga taaacgttta gacctctcgt tacaaccttt ggggtttgct aaggtgactg
     2881 gtattactga cgatattttg caacgcctaa agcaacaaaa aggttatttg atgttgagcg
     2941 acaaaagtag tcctgatgaa atttatgctg cctttggcgt gagtaaaaag gtatttaaac
     3001 aagcaattgg tacgttattt aaacaacgtc aaattattat cgaagaacaa gggattcgct
     3061 tagcgaatcg taaaatttag agtgtattca atacattaca acttaggact tacaccatta
     3121 tcgcttattg gctcgtattt ttaacgattt tatgctcaca aaatgtgcgc ctgaaggtga
     3181 a
//
LOCUS       DAOQMB010000266         3173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16692_length_3173_cov_18.780949, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000266 DAOQMB010000000
VERSION     DAOQMB010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16692_length_3173_cov_18.780949"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..773)
                     /locus_tag="PLY05_08590"
     CDS             complement(54..773)
                     /locus_tag="PLY05_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03396.1"
                     /translation="MLSIAHPIIRFPLILMAAAFAIWATQTGIAGLYQFQAQSHFETW
                     QNLRLSKPNYTVSPEEYQDISEKYRQSIKLTPYNADFYTAFADMQLWYLANTPNIPIQ
                     EQKNIKANILSHYRTALAQRPTWPYSHIYFAVIKARFGDIDNEFIQSLHTANKLGKNE
                     VDVIRITVELGLALWSQLDQNTHKLIANAIERSIAWNLNEQFNNKERIFALSLVGMYQ
                     KQKEICALMSIENQKKSNMCD"
     gene            1093..1338
                     /gene="rpsP"
                     /locus_tag="PLY05_08595"
     CDS             1093..1338
                     /gene="rpsP"
                     /locus_tag="PLY05_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016140011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNC03397.1"
                     /translation="MVVIRLARGGAKKRPFYQVVVTDSRNARDGRFIERVGFFNPVAQ
                     GAAEKVRLDTDRYNYWISQGAQPSDRVAQLAKTAVTA"
     gene            1346..1879
                     /gene="rimM"
                     /locus_tag="PLY05_08600"
     CDS             1346..1879
                     /gene="rimM"
                     /locus_tag="PLY05_08600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNC03398.1"
                     /translation="MAIPAIDQLLTIGRIQSAYGIKGWVWIYANTEPMDNVFAYTPWY
                     LQIGKDWREIEVVEWREQGKGIIARLAGCNDRNAAEALHNAIIWTAKANLPDLPEGDY
                     YWSDLVDLQVWTVNGQLLGQVYSLMETGANDVLVVRACAGSIDSQERLIPWIPEQVVT
                     AVDIVNRRLVVDWDIDF"
     gene            1897..2667
                     /gene="trmD"
                     /locus_tag="PLY05_08605"
     CDS             1897..2667
                     /gene="trmD"
                     /locus_tag="PLY05_08605"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNC03399.1"
                     /translation="MWFGVITLFPEMFKAISDFGMTGRAVKHNLLQLEMWNPRDFTED
                     NYRRVDERPYGGGPGMVMLIEPLAKAIVAAKARAASLGISAPVIYLSPQGQQFNEIAT
                     QQLAKAQGFILLCGRYEGVDERLLAQYVDAEWSIGDYVLSGGELPAMVLIDAISRRID
                     GVLGDKQSAEQDSFVSGLLDCPHYTRPEVYQGHAVPDVLLSGNHALIQRWRWQESLRR
                     TLIRRPDLLASATLNAEQRRLLTQIKHELSNDDIGQSN"
     gene            2804..3172
                     /gene="rplS"
                     /locus_tag="PLY05_08610"
     CDS             2804..3172
                     /gene="rplS"
                     /locus_tag="PLY05_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNC03400.1"
                     /translation="MSGKHPIIQSIEQAQMKQDVPAFGAGDTVVVQVKVKEGDRERLQ
                     AYEGVVIAKKNAGLNSAFTVRKISNGVGVERVFQTHSPLVASIEVKRRGAVRRAKLYY
                     LRDRTGKSARIKEKLVARAK"
BASE COUNT          862 a          543 c          740 g         1028 t
ORIGIN      
        1 tgagtattgg ttggttttaa ggcaagggat tgggagaact ttattgagtt ctcctaatca
       61 cacatattcg attttttctg attttctata gacattaaag cacagatttc tttttgtttt
      121 tgatacattc ccacaaggct tagagcaaaa atacgttctt tgttgttgaa ttgttcattt
      181 aaattccatg caattgaacg ttcgatagca tttgcaatta gcttatgagt gttttgatct
      241 aattgagacc acaaggctaa tcctaattcg acagtaatac gaatcacatc aacttcattt
      301 ttgcctagtt tattggcagt atgtaaggat tggataaatt cattatcaat atcaccaaaa
      361 cgggctttaa tcactgcaaa ataaatatgg ctatatggcc atgtggggcg ttgtgccagt
      421 gcagtgcggt aatggcttaa aatatttgct ttgatgtttt tttgttcttg gataggaatg
      481 ttgggggtat tggctaagta ccacagttgc atatctgcaa aagcggtata aaaatcagcg
      541 ttgtaggggg ttagcttaat gctttggcgg tatttttcgc taatgtcttg gtattcttcg
      601 gggctaacag tgtagtttgg tttgcttaga cgcaagtttt gccatgtttc aaaatggctt
      661 tgtgcttgaa attgatacag accagcaatc cctgtttgtg ttgcccaaat tgcaaaagct
      721 gcggccatta aaattaatgg aaaacgaatg attggatgag caatagacaa cataacggtg
      781 atttctgagc gaaaagcaaa aaataatctt cgctatatac cataactttg tcgcttttgt
      841 tagctaatct cttttataaa agcgcaaatt cccgtacaat gctcgccctt tgaagactac
      901 gatcttaaaa gtaggccgat gtttaatcat aaattgataa aatcacctat tttagagtga
      961 gtcttgattt gtaggactga cgcggttatc gctgatctgt tcgttaaatc atctttagat
     1021 gatgtataac gagcaaaacc cgctagttgc gatgcaaagc gtaagtcata ttcttttttg
     1081 tggaatacaa ttatggtcgt tattcgttta gctcgtggcg gtgccaaaaa gcgtcctttt
     1141 tatcaagttg ttgtgactga cagtcgcaat gctcgtgatg gtcgttttat tgaacgtgtt
     1201 ggttttttca acccagttgc tcaaggcgct gctgaaaaag tgcgtttaga cactgatcgt
     1261 tataactatt ggatcagcca aggtgctcaa ccttctgatc gtgttgctca attagcaaaa
     1321 actgctgtta ctgcttaatt gtgtgatggc cattcctgca attgaccaat tattaacaat
     1381 tggccgtatt caatctgctt atggcattaa aggttgggta tggatttatg ctaatacaga
     1441 gccaatggac aatgtctttg cttatacccc atggtatttg caaattggta aagattggcg
     1501 tgaaattgag gttgttgagt ggcgagaaca aggtaaaggt attattgcac gtttggctgg
     1561 ttgtaatgat cgtaatgctg ctgaagcttt acacaatgca attatttgga cggcaaaagc
     1621 taatttacca gaccttcccg agggtgatta ttactggtca gacttagttg atttacaagt
     1681 ttggacagtg aatggtcaat tattagggca ggtgtatagc ttaatggaaa cgggtgccaa
     1741 tgatgtgtta gttgttcgcg cttgcgcagg cagcatagac agtcaagaac gccttattcc
     1801 gtggattcct gaacaagttg tgacagctgt tgatattgtt aatcgccgct tggtggtgga
     1861 ttgggatatt gacttttaaa tcgcgagaac acggtgatgt ggtttggcgt tattacgcta
     1921 tttcctgaga tgtttaaagc catttctgac tttggcatga cggggagagc agtaaagcac
     1981 aatttgcttc agttagaaat gtggaatcca cgcgatttta cggaagataa ttatcgtcgg
     2041 gttgatgagc gtccctacgg cggtgggccg ggtatggtga tgttaatcga gcctttggcg
     2101 aaagcaattg ttgctgcaaa agcacgtgct gctagtttag gtatttctgc gcctgtcatt
     2161 tatttgtcgc cacaagggca acaatttaat gagatagcga cacagcaatt agcaaaagct
     2221 cagggcttta ttttgttgtg tggtcgttat gaaggtgtag atgagcgttt actagcacaa
     2281 tatgttgatg cggaatggtc aattggcgat tacgtgttgt ctggtggtga gctaccagcc
     2341 atggtgctaa ttgatgcaat cagcagacgt attgatggag tattagggga taaacagtca
     2401 gccgaacagg actcttttgt gtctggtttg ttagattgcc cacattatac tcggccagaa
     2461 gtgtatcagg gacacgctgt tcctgatgta ttactttctg gtaatcatgc actgattcag
     2521 cggtggcgat ggcaagaaag tctgcgtcgt actttaatac gtcgtcctga tttgttagcc
     2581 tctgcaactt tgaatgcaga gcaacgtcga ttgttaactc aaatcaaaca cgagttaagc
     2641 aatgacgata taggtcagtc taattaatga tagaattttg cccagtgctt aagttctaat
     2701 taagactgtg aattaacagt tatatgcgcc gagtaatgac tcatagcctc tgtaacttat
     2761 cagtatttat aatcacatac tgattctttt taggagtttt accatgagtg gtaagcaccc
     2821 aattattcag agcatcgaac aagctcaaat gaaacaagac gtacctgctt ttggtgctgg
     2881 tgacactgtt gttgtccaag taaaagtaaa agaaggtgat cgtgagcgtt tacaggccta
     2941 tgaaggtgtt gtaattgcta agaaaaatgc aggtttaaac tctgctttta ccgtacgtaa
     3001 aatttctaac ggtgttggcg ttgaacgtgt tttccaaacg cactctccat tagtggcttc
     3061 tatcgaagta aaacgccgtg gtgctgtacg tcgtgctaaa ttgtactacc tccgtgatcg
     3121 tactggtaag tctgcacgta ttaaagaaaa attggttgct cgcgctaaat aat
//
LOCUS       DAOQMB010000267         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_16943_length_3148_cov_12.202069, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000267 DAOQMB010000000
VERSION     DAOQMB010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16943_length_3148_cov_12.202069"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..2786
                     /locus_tag="PLY05_08615"
     CDS             15..2786
                     /locus_tag="PLY05_08615"
                     /inference="COORDINATES: protein motif:HMM:NF028103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HNC03401.1"
                     /translation="MTDYSRSNILLPHFEEAEAFLNALDGKDAQFTFQTFDDVEVWSD
                     EKQKTIKRMDKSLVRVCHGTFNEHKAKLAELNKLGGGVFVTINETDLTGRKKENILKV
                     RAVFVDLDGSPLQPILDLEEDLQPHIIIESSPNKWHAYWLVDNCSLELFPHVQRALAA
                     KFNGDIAVNNINRVMRLAGFSHNKGESFITRIHTMQDDLYPYSVNKLIVGLGLELEQP
                     TQKKQTFDSFSNATHSGDFETVKRAAQGRWAAIFSRLGIALPPDNKHAPCPACGGTDR
                     FRYDNQDGHGTFICNQGGNGILSGDGFALIQHKTGATSSEVLNMVRGIVMPDYQKSKK
                     YQSANGGEDKIISHIDNSGHKKSQPTVLIEMADDCELFHTSDDEAYAVVKTPTSRQVF
                     AVRSAGFRRYLSQRYFKLFGKGVGNQALQDALITIEAKANFNGTLRQVYKRVANLGDR
                     IYLDLCDEKWRVIEVTAAGWQVLNCSPVMFTRTKDMLSIPEPKNDASDAILSVNDLVK
                     NKEEKYKNIYLLKRFVNVRDEDYPLIYAFILACLRGIKPYPVLLLQGESGTGKTTTAR
                     NMRGLIDPSKADVLGKPKDENELKTCANNSYILAIDNLSGLSKDFSDVVCQIATGASS
                     SKRKLYTDNDVETYEIARPIILNGIDDIASRGDLLNRSFQVNLPALNDDKKGDEQELA
                     TQYNQAKPVILAAFLDMLSVGLRDYDKIEKIKTSGRFVSLPRFVTAAERGLGLSELFA
                     KTYDQNTKQGVELALETSPLAQALITFMSLKTIWTGTIGELFQQLNDRFKTDFLTLQS
                     GSWPKTPRYLSGELTRLAPQLRQTHIDIKTKKESHGMTITLEKVRKIASLASLASLNN
                     KNNELQDEPSVIVASGLHHSSVINDEVASFSIIEEPSHIKGYDANDANDARFSKNSNN
                     SAMTWEADL"
     gene            2783..>3148
                     /locus_tag="PLY05_08620"
     CDS             2783..>3148
                     /locus_tag="PLY05_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03402.1"
                     /translation="MTAQDVLKMAFDCGVLIQVKGDKLHLEAKEAPPNHLVAEIRHYK
                     ADLIALLAANDPVKNESYPPLEDGQLNRKSADKQSPKNLGDALKTEQTTAPNFRDTFT
                     PTAETSAVEQQPPFSLNNYS"
BASE COUNT         1022 a          627 c          643 g          856 t
ORIGIN      
        1 acaggtaatt aacgatgact gattatagcc gtagcaatat cttattgcca cattttgaag
       61 aagccgaagc ctttttaaac gccttagacg gtaaagacgc gcaatttaca ttccaaacct
      121 ttgatgatgt tgaagtgtgg agtgatgaaa agcaaaaaac cattaaacgc atggataaaa
      181 gcctagtgcg cgtttgtcat ggcacattta acgaacacaa agccaaactt gccgagctta
      241 acaaacttgg tggcggtgta tttgtcacga ttaacgaaac cgacctaaca gggcgcaaaa
      301 aagaaaatat cttaaaagta agagccgttt ttgttgattt agacggttca ccacttcaac
      361 cgattttaga cctagaagaa gatttacagc cccatattat tattgaatca agccctaata
      421 aatggcacgc ctactggctt gtagataatt gcagcctaga actattccct catgttcaac
      481 gtgccttagc cgctaaattt aacggtgata tagcggtgaa taacattaac cgtgttatga
      541 gacttgcagg gtttagccat aacaaaggcg aatcatttat cacgcgcatt cataccatgc
      601 aagatgacct ttacccctac agcgtgaata agttaattgt tggtttaggt ttggaattag
      661 aacagccaac acaaaaaaaa cagacttttg atagcttttc taatgctact cactcaggcg
      721 attttgagac cgtcaaacgt gcagcacaag gacgatgggc tgctattttt agccgtcttg
      781 gtattgcact accacccgat aataaacacg cgccttgccc tgcttgtggc ggtacagacc
      841 gctttagata tgacaatcaa gatggacacg gtacatttat ttgcaatcag ggcggcaatg
      901 gtattttgtc aggtgatggt tttgcattaa ttcaacacaa gacaggtgca accagtagcg
      961 aagtcttaaa catggttcgc ggtattgtta tgcctgatta tcaaaaaagt aaaaagtacc
     1021 agtctgctaa tggtggcgag gataaaatca ttagccatat tgataattca ggccataaaa
     1081 aatcacagcc tactgtactt attgaaatgg cagacgattg tgagttattt cacacgtctg
     1141 atgatgaagc ctacgccgtt gttaaaacgc caacatcacg gcaagtattt gctgtccgta
     1201 gtgcaggttt tagacgttat ttaagtcagc gttattttaa attgtttggt aaaggtgtcg
     1261 gcaaccaagc cttacaagat gcactaatta cgattgaggc aaaagccaat tttaacggca
     1321 cattgcgtca agtatataaa cgtgtagcta atcttggcga ccgcatttat ttagatttgt
     1381 gtgatgaaaa atggcgcgtt attgaagtca cagccgcagg ttggcaagtg cttaattgct
     1441 caccagtcat gtttactcgc acaaaagata tgctatccat tcctgagcca aaaaatgacg
     1501 ctagtgacgc tattttatct gtgaatgatt tagtaaaaaa taaagaagaa aagtataaaa
     1561 acatatactt actcaagcgt tttgtgaatg ttcgtgatga agactatcca cttatttatg
     1621 cgtttatatt ggcttgcttg cgcggtatca agccgtaccc cgttttatta ttacaaggtg
     1681 aatcaggtac aggaaaaacg accacagcgc gaaatatgcg cggtttaatt gacccatcaa
     1741 aagcagatgt tttaggtaag cctaaagacg aaaatgagct taaaacctgt gctaataact
     1801 cttacatttt ggcaattgat aatttatcag gcttatcaaa agatttttct gatgttgttt
     1861 gtcagattgc aacaggtgca tcaagtagca agcgcaaact ttacacagat aatgacgtag
     1921 aaacttacga aattgcccga cctattattc tgaatggcat tgatgatatt gcctctcgtg
     1981 gcgatttatt aaaccgtagt tttcaagtga atttacccgc attaaatgat gataaaaaag
     2041 gcgatgaaca agagttagct acccaataca atcaagctaa acctgttatt ttggccgctt
     2101 ttttagatat gttgtctgtg ggattgcgtg attacgacaa gatagaaaaa ataaaaacca
     2161 gtggccgctt tgtcagtctg ccgcgctttg taaccgcagc agaaagaggc ttaggactgt
     2221 ctgagttatt tgcaaaaacc tatgaccaaa acactaaaca aggtgttgaa ctagcactag
     2281 agacttcgcc attagcgcaa gcattgataa cttttatgag ccttaaaaca atatggactg
     2341 gcacaattgg cgaattattc caacaactca atgaccgctt taaaactgac tttttaacat
     2401 tgcaatcggg ttcgtggcct aagactccaa gatatttaag tggcgaatta acacggcttg
     2461 ctccacagct acgccaaacg cacatagata ttaaaactaa aaaagaatct catggtatga
     2521 ccattacttt agaaaaagtt agaaaaatag cgtcattagc gtcactagca tcattaaata
     2581 ataaaaacaa cgagttacag gacgagccta gcgtcattgt agcgtcagga ttgcatcatt
     2641 ctagcgtcat aaatgatgag gtagcgtcat ttagcatcat agaagaacct agtcatatca
     2701 agggttatga cgctaatgac gctaatgacg ctagattctc aaaaaattct aacaatagtg
     2761 ctatgacttg ggaggctgat ttatgaccgc ccaagacgta ctaaaaatgg cttttgattg
     2821 tggtgtactt attcaagtaa aaggcgataa attgcatctt gaggccaaag aagcaccgcc
     2881 taatcattta gtggcagaga tacgccacta caaagcggat ttaattgcgt tactggctgc
     2941 taatgaccct gttaaaaatg aatcctatcc cccattggag gacggacagc taaaccgcaa
     3001 aagtgcggac aagcaatcgc ctaaaaattt aggagatgcg ctcaaaaccg agcagaccac
     3061 cgccccaaac tttcgggaca cgtttacacc aacggcggaa acctccgcag tggagcaaca
     3121 acccccattt agcctaaaca attacagt
//
LOCUS       DAOQMB010000268         3115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_17274_length_3115_cov_14.659804, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000268 DAOQMB010000000
VERSION     DAOQMB010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17274_length_3115_cov_14.659804"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="PLY05_08625"
     CDS             <1..414
                     /locus_tag="PLY05_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HNC03403.1"
                     /translation="VGVSYDKDNNRSCVLRVSATGFSVLLTGDMEAPAEQILLKQQKN
                     LQSDILLLGHHGSKTSSTEAFVQAVQPKLAIVSAGYLNHFGHPAPMVVQRLQKRQIDI
                     DNTIEQGTLRYHLHKQGFILQEAWRNRGHYWLNMP"
     gene            536..1123
                     /locus_tag="PLY05_08630"
     CDS             536..1123
                     /locus_tag="PLY05_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC03404.1"
                     /translation="MLPILIASVLAMAICFERAWTLRASRVAPPNLLAEVWGWIKSNQ
                     LDAEKLRQLKANSPLGEILAAGLLNSRHGREIMKECIEESANVVIHHMERYLTVLGTL
                     AMISPLLGLLGTVVGIIEAFMAINSSGMGDPALLASGISKALITTAGGLFVAIPAMIM
                     HRYFARHITTMAVEMEQQAIKLVDIVHGDREVDFK"
     gene            1195..1620
                     /locus_tag="PLY05_08635"
     CDS             1195..1620
                     /locus_tag="PLY05_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005177483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC03405.1"
                     /translation="MKFRRPQPENLEINLTPLIDCLLFLIIFLMISTTFNKASKLQIV
                     LPEAKGDVAKPSKAKSIEIGVSAGGVYTINGQELAVKDAKQLQSAIEKISDGKRDMPF
                     IIAADGKASHQAVITVMDVAGQMGFVNLSISTRLASENP"
     gene            1620..>3115
                     /locus_tag="PLY05_08640"
     CDS             1620..>3115
                     /locus_tag="PLY05_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HNC03406.1"
                     /translation="MTQATGWQTYKRLLGYSKAHWKAFVLGVLGFILNAQTEWAAAEQ
                     IKFIINAIQNKSQADKNLFPLLVIAIFFFRGVGTFMGTYYLSLVARNVVYQLRKELFA
                     KLLVLPNHYYHLHSAGHLSAKLIYDVEQVTEAASEALKTLVREGFVVIGLLGYLFYTN
                     WRLSISLLIIGPIAAALVRTASKRFRKLSHKIQNTMGDVNHIVNETIQGFAVVKSYGG
                     QEFETKRFEQASQENLKQSMKMVVTSALNTPIIQFLMAIAMGGVVWLALQPHILGDVS
                     AGAFVAYITAAGLLSKPVRSLTDVNSKIQKGIAAAQSVFDLLDCPVEPDTGTYQAKKV
                     EGRLEFKNLSFSYPTGDTVLHNIDLTIPAGKTVALVGRSGSGKSTLVSLIPRFYETTE
                     GQILLDGHALSEYTLANLREQIATVSQKVMLFDNTIRHNIAYGDLANKTDAEVEAAAK
                     AAYAHDFIMKLPEGYQTRVGQDGTQLSGGQRQRIAIARALLKNAPILI"
BASE COUNT          884 a          550 c          710 g          971 t
ORIGIN      
        1 gttggggtaa gttatgataa agataataac cgttcttgtg tgttaagagt cagtgcaaca
       61 ggatttagtg tgttgttaac aggtgatatg gaagctccag ccgaacaaat tttattaaaa
      121 cagcagaaga atttgcaaag tgatattttg ttgttggggc atcatggtag caaaacctca
      181 agcacagagg cttttgtgca agcggttcag cccaaactgg cgattgtttc agcaggttat
      241 ttaaatcatt ttggtcatcc tgcacctatg gtggtgcaac gattacaaaa acgacaaata
      301 gacattgata atacgataga gcaaggcaca ctgcgttatc atttgcataa acagggattt
      361 attttgcaag aagcttggcg taatcgtggt cattactggc taaatatgcc ttaaatggtt
      421 aaagtatgcg gtcttgacgt tgggcttttc gcgtttacat ctatgctagt ccgatcaaat
      481 tttcaggtat gaggtataaa acgtgtggga attagttaaa tctggcggcc tcttaatgtt
      541 gccaatttta attgcttcag tattagcaat ggcgatttgt tttgaacgtg catggacatt
      601 aagagcaagt cgagttgcac cacccaattt attagccgag gtttggggtt ggattaaaag
      661 caatcaatta gatgctgaaa agttacgtca gctcaaagct aattcccctt taggcgaaat
      721 tttagcggct ggtttattaa attcgcgtca tggccgcgag attatgaaag agtgtattga
      781 ggaaagtgcc aatgtggtta ttcatcacat ggagcgttat ttaacggttt taggcacatt
      841 agcgatgatt tcgccattgt tgggtttgtt ggggacagta gttggcatta ttgaagcttt
      901 tatggccatt aatagctcag gcatgggcga tcctgcatta ttagcgagtg gtatttctaa
      961 agccttaatt accactgcag gtggtttgtt tgtggcaatt cctgcaatga ttatgcaccg
     1021 ttattttgct cgtcatatca ccacaatggc ggttgaaatg gaacaacaag ccattaaatt
     1081 ggtcgatatt gttcatggtg atcgtgaagt cgattttaaa taatagagcc tcacttgttg
     1141 tgttccctct cctttgggag agggctaggg tgagggtaat atagagtcta gtgtatgaaa
     1201 tttcgtcgtc cacaacccga aaatttagaa atcaacctaa cgccattgat tgattgtttg
     1261 ttgtttttaa ttatcttttt gatgatttct accactttta ataaagccag taaattacaa
     1321 atagtcttgc ccgaagctaa aggggatgta gcaaaaccca gcaaagccaa gtcgattgaa
     1381 attggcgtga gtgcgggtgg ggtttatacc attaatggcc aagaacttgc cgttaaagat
     1441 gccaaacaat tacaaagtgc gattgaaaaa atcagtgatg gcaaacgaga tatgcccttt
     1501 attattgccg ctgatggtaa agctagccat caagccgtga ttaccgtgat ggatgttgca
     1561 gggcaaatgg ggtttgtcaa tttaagcatt agcactcgtt tggctagtga gaatccataa
     1621 tgacacaggc aacaggttgg caaacttata aacgcttatt aggttatagc aaagcacatt
     1681 ggaaagcgtt tgtattgggt gtattaggct ttattctaaa tgcccaaacc gaatgggcgg
     1741 ctgccgaaca aatcaaattt attattaatg ccattcaaaa caaaagccaa gccgataaaa
     1801 atttgtttcc attattagtg attgccatct tcttttttag aggtgttggc acttttatgg
     1861 gtacgtatta tttgtctttg gtggcgcgta atgtggtgta tcagttacgc aaagagctat
     1921 ttgctaaact tttagtctta cctaatcatt attatcatct acattcggca gggcatctta
     1981 gtgccaaatt aatttatgat gtcgagcaag tgactgaagc cgctagtgag gccctaaaaa
     2041 ccttagtgcg cgaaggtttt gtggtgattg gtttattagg ttatttgttt tataccaact
     2101 ggcgactctc tatctcattg ttaattattg gcccaattgc cgctgcgttg gtacgtactg
     2161 ccagcaaacg ctttcgtaaa ttgtcacaca aaatccaaaa taccatgggc gatgtgaatc
     2221 atatcgtcaa tgaaaccatt caaggttttg cagtggtcaa aagttatggt ggccaagaat
     2281 ttgaaaccaa acgttttgag caagcttcgc aagaaaatct gaaacaaagc atgaaaatgg
     2341 tagtcacttc ggcactcaat acaccaatca ttcagttctt aatggcaatt gccatgggcg
     2401 gtgtggtgtg gttggccttg caaccacata ttttgggtga tgtgtccgca ggtgcatttg
     2461 tcgcttatat taccgccgca ggtttgttgt ctaaacctgt ccgttcttta accgatgtga
     2521 atagtaaaat tcaaaaaggt attgccgcag cgcaatcggt ttttgattta ttggattgtc
     2581 ctgttgaacc agatacaggg acttatcaag ccaaaaaagt ggaaggtcgt ttagagttta
     2641 agaatttgtc gtttagttat cccacaggtg acactgtctt acacaatatt gatttaacca
     2701 ttcctgcggg taaaacagtg gctttagttg gccgttcagg ctctggtaaa agtactttgg
     2761 tgagtttgat tcctcgtttt tatgaaacca cagaaggcca gattttatta gatggccatg
     2821 ccttgtcaga atacacctta gcgaatttac gtgagcaaat tgctaccgtt agccaaaaag
     2881 tcatgttgtt tgataatacc attcgtcaca atattgctta tggtgatttg gccaataaaa
     2941 ccgatgccga agtagaggct gctgctaaag ccgcgtatgc ccatgatttt attatgaagt
     3001 taccagaagg ctatcaaacg cgagttggtc aagatggtac acaactatca ggtggacaac
     3061 gtcaacgtat tgctattgcc cgtgccttat taaaaaatgc gccaattcta atttt
//
LOCUS       DAOQMB010000269         3102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_17411_length_3102_cov_3.002297, whole genome shotgun sequence.
ACCESSION   DAOQMB010000269 DAOQMB010000000
VERSION     DAOQMB010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17411_length_3102_cov_3.002297"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3102)
                     /locus_tag="PLY05_08645"
     CDS             complement(<1..>3102)
                     /locus_tag="PLY05_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004661761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC03407.1"
                     /translation="ASDTTTKSSRSSRTNESRTPINRERDSAIVRTSTRAGSVTQTAK
                     EAEQLKAEGTPTQWGDAANIDKALPYLLPEQRDDVAKLEKRLIEENKNGMLFTNSTGT
                     GKTFTGLGAVKRFANAGKKNILIVSMNDKIIRDFIKSAKSLNLDVHQLDGITDNGKDK
                     IVATTYANMAQNNTLGMRDWDLIVVDEAHNLMQGEKGEDTNALAKLRALSGHHAGFHE
                     WVKMRHADKDPKPIGEDEEGRPIYDQEQVAAWEAFKKPLREQWQKTWTEQPKGRTKVI
                     FLTATPFSYVKTLDWAEGYLFDFVPPADKFSNKTDAMGLAYNSGGAQERFYMNNFGYK
                     MRYNRLTRPDGRVDVGILERKFAEKLKTEGAMSGRELSVPFDYDRKFVLVNSKIGEKI
                     DEGFKFLWDTKGKDGRSKYSDLLRVINKRFDYLARLQLLEAIKAEEAIDQIKKHLALG
                     RKVIVFHDFNEGGTTNPFVMKGFSGDDASAVEAQYDDFKRERPDLINLKLDIPAPLVT
                     LRRAFSNALMFNGTVSKGQRAKNADLFNSDDSGLNVMIAQSDAAATGISFHDTTGKHQ
                     RVIINIGMPTKPAKLRQTEGRIYRVGQASNAIQRYLTTGTGWERSAFAQKIAERAETV
                     DNLAQGESAVVSIKDALVRAYEEAEYFEPSENDGVGGKAYDEENSRVMALSPFERAKT
                     DYWVKQKVTAKRNYREGKEWYATPEPVGLFMVNLAGAHSGDDILEPSAGDGAIGRYMP
                     SDASVTFIEPSDSLASRARMNNTNANVIVDDFESHGSNNKYDAIVMNPPFGQGGSIAI
                     KHLVKAFEHLRDGGRVVALLPVGKMDELIAKYQADGYFDKIYTVAEFTLPSSTFENAG
                     TRVNTKIHVFEKHENKADAPQGMIRKDLSHYDDISGLFDAIENINIKPRKPRLDEALA
                     EYGIQVYPERSKYIVTGEGLKRMDIKSLLFTPYDKPNADGDYVEKYNYSKQYLRLLKE
                     NNTATTEQFNQGLSANNLPIKYQDPRSGFKHNVWDGFGEKPYWLTQALQYGKKLEDFA
                     VEKP"
BASE COUNT          789 a          715 c          594 g         1004 t
ORIGIN      
        1 aggcttttct acggcaaaat cttctagttt tttaccatat tgcaaggctt gtgttagcca
       61 atatggtttt tcaccaaaac cgtcccaaac attatgctta aagccgcttc gtgggtcttg
      121 gtatttaatc ggcaaattgt ttgcgcttaa tccttgatta aattgctcgg ttgttgcggt
      181 attgttttct ttgagaagtc ttagatattg tttgctgtag ttgtattttt caacataatc
      241 accgtcagcg tttggcttgt cgtaaggggt aaataacagg ctcttaatat ccatccgttt
      301 taacccctca ccagtcacaa tatacttgct acgttcaggg taaacttgaa taccatactc
      361 ggctaaggct tcatctaagc gaggcttacg cggcttaatg ttgatattct caatcgcatc
      421 aaaaaggccg ctaatatcat cgtaatgcga taagtctttt ctaatcatgc cttgaggcgc
      481 atcagcttta ttttcgtgct tctcaaaaac atgaatttta gtattaacgc gagtacctgc
      541 attttcaaag gtggagcttg gtaatgtaaa ttcggctacc gtgtaaattt tgtcaaaata
      601 accatcggct tgatatttcg caatcaattc atccattttg ccaacaggca ataacgcaac
      661 cacacgccca ccatctcgca aatgctcaaa ggcttttact aggtgtttaa tggctatgct
      721 tccaccttga ccaaaaggcg gattcatcac aatagcatcg tatttgttgt ttgagccgtg
      781 actttcaaag tcatcaacaa tcacgttagc attagtgtta ttcattcgcg cacgactagc
      841 caaagaatcg ctaggttcaa taaacgtaac actggcatca cttggcatat aacggccaat
      901 tgcgccatct cctgcgcttg gctctaaaat atcatcgcca ctatgcgccc ctgctaaatt
      961 aaccataaac aagccaacag gttcaggcgt tgcataccac tctttgcctt cacggtagtt
     1021 acgttttgcg gttacttttt gctttaccca ataatcggtt tttgcacgtt caaagggtga
     1081 taaggccatg actcgactat tttcttcatc ataggctttt ccccctacac cgtcattttc
     1141 tgagggttca aaatactctg cttcctcata ggcgcgtact aaagcgtctt tgatactaac
     1201 aactgctgat tcaccttgag ccaagttatc gacagtctcc gcacgttctg caatcttttg
     1261 agcaaaggca ctacgttccc aacctgtacc agtagttaaa tagcgttgaa tagcattaga
     1321 ggcttggcct acacgataaa tacggccttc ggtttggcgt agtttggctg gctttgtcgg
     1381 cataccaata ttgataatca cgcgctgatg cttgcctgtc gtatcgtgaa aactgatacc
     1441 tgtagcggct gcatcggatt gggcaatcat cacatttaag ccgctatcat cactattaaa
     1501 aaggtcggca tttttggcgc gttgaccttt agatactgta ccgttaaaca ttaaggcatt
     1561 agaaaatgct ctacgcaagg taactaatgg agctggaata tctaatttaa ggttgattaa
     1621 atcggggcgt tcacgcttaa agtcatcgta ttgcgcttct actgctgacg catcatcacc
     1681 tgagaatcct ttcatcacaa aggggttagt tgtaccaccc tcgttaaaat cgtggaatac
     1741 aatcacttta cggcctaaag ccaagtgctt tttaatttgg tctatggctt cttcggcttt
     1801 gatggcctct agtaactgta aacgtgctaa atagtcaaaa cgcttattga taactcttaa
     1861 taaatcgctg tatttgcttc taccgtcttt gcctttggtg tcccataaaa acttaaagcc
     1921 ctcatcaatt ttttcaccga ttttgctatt aactaacaca aacttacggt cataatcaaa
     1981 cgggacagac aattcacggc ctgacattgc cccctctgtt tttagctttt cggcaaactt
     2041 acgctccaaa atacctacat caacacgacc atcggggcgc gttaatcggt tataacgcat
     2101 tttatagcca aaattattca tgtaaaagcg ttcttgtgcg ccacctgaat tataagctaa
     2161 tcccattgca tctgttttat tgctaaattt atcggctggc ggcacaaaat caaacaaata
     2221 gccttctgcc caatctaatg ttttaacgta gctaaatggg gttgctgtta aaaagataac
     2281 cttagtacgg ccttttggtt gctctgtcca agttttttgc cattgttcgc gtaatggctt
     2341 tttaaaagcc tcccatgccg caacctgttc ttggtcgtaa atcggtctgc cttcttcgtc
     2401 ttcaccaatt ggcttagggt ctttatccgc atggcgcatt tttacccatt catggaaacc
     2461 tgcatgatgg cctgataagg cgcgtaactt agctaaagca tttgtatctt cgcctttttc
     2521 accttgcatg aggttgtggg cttcatcaac aacaattaaa tcccaatcac gcataccaag
     2581 cgtattgttt tgcgccatat ttgcgtatgt tgtagcgaca atcttgtctt tgccgttgtc
     2641 ggtaataccg tctaattggt gtacgtctaa attaagcgat tttgcagact taataaaatc
     2701 acgaataatt ttgtcattca tggacacaat taagatgttc tttttgcctg cattagcaaa
     2761 acgctttacc gcgcctaaac ctgtaaaggt cttacctgta cctgttgaat tggtaaacaa
     2821 catcccattt ttattttctt cgattaaacg cttttctagc tttgccacgt catcgcgctg
     2881 ttcaggcaac aaatagggca atgctttgtc aatgtttgca gcatcgcccc attgggtagg
     2941 tgtaccctct gcttttaact gttccgcctc tttagcggtt tgggttacgc ttccagctcg
     3001 agtagaagtg cgtacaattg cgctatctcg ttctctgtta ataggtgttc gtgactcatt
     3061 agtgcggctg cttcggctgc tcttagtggt ggtatcactt gc
//
LOCUS       DAOQMB010000270         3093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_17509_length_3093_cov_5.053983, whole genome shotgun sequence.
ACCESSION   DAOQMB010000270 DAOQMB010000000
VERSION     DAOQMB010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17509_length_3093_cov_5.053983"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..>3093
                     /locus_tag="PLY05_08650"
     CDS             46..>3093
                     /locus_tag="PLY05_08650"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HNC03408.1"
                     /translation="MERALEATDKIINANISYVKAFNTTPQKHKSLVKELLSALGDTV
                     GEGIIETDAKLLAEAIYNKDYKIEHSTKFYEGMSQYAVKVLQTSDVIAALNPVKGDDV
                     DALKVIDAITHNEFFQEIFSTVIQKAASSAAKAMFKGGFRKKAAQFNVASKAASLTWG
                     IGTKLLPMMWDSCTTSKKLSIGIVNGKISQYQAPKVDLWIEDLTTGKPVYWYNNMGES
                     LSASGDTLTAIQGRKYRIHLKSKQAGILDYSLDNNKSDAVHKSWIRTPVKVNGLFIRR
                     TTVENENQILAHKQLCARRALQDGNTMVYGLNQFTTLSGFACVEGQVGTGDAQLFSPP
                     FNQTQGFVQHNGNVNYATTTYREGLGFSSWQELTSGATKNLGFGGEVSDTFDFTEDKL
                     TKSLFIEIEGFNDEIQQGEDAKQIFDIRVVNNADAVISSNAKPSAANGPLYVGDAITA
                     KVTQIVGKVVSVFWQLINSLGEVVKSMSAVVAESASFATDNLSGGTYEIKAELRDENA
                     QPIGVVSQSIPLLQTDFKSVNVQSMPVGVPVAITVYGKDLPSTTVFAVEGVLCEQPLI
                     DGVSVTQICTASEVKNNVKLTVKRKTQVDEGSRIFSINITPKSVAGQFVVPATSTGVE
                     FIPPIDNTQRVSELGLVVGYGASRRNMTCSFTSTGNTKTYKDYPDVDANGYSTDILPL
                     LNGFANMALLATKQQGSNTQTLLIGNSQTVSIAAGQTLNFYVNDDNYGDNEGALTVNY
                     QCTQLTATIQPSTTTPVAQSPITIGFVDTVIANVKDVVWAVSDAVSEIFVSVRNGLSE
                     LFDYSFSKSGDYKVTATVRDANNQTIATSSTQLTVTAAPPTASLLGSATTGIVGQPVV
                     FVLNNSKALEGKICSYLFAGGGQIGDSGGGDCSNASQVLVLPSITELYTTAGTYTATL
                     TVTDSQGKTATTSHIITVTEATQTASTGLLNDTGITQCSDQSTLFADCTAASMGGWFG
                     LNQDGETGRDLLAVKGTLAKTGAGDAGFDFTKISATGLPLPASA"
BASE COUNT          957 a          663 c          692 g          781 t
ORIGIN      
        1 agaaataagc attaaatcta taaaaagtag atttgagtaa gacttgtgga acgggcttta
       61 gaggcaaccg acaaaataat taatgccaat ataagctatg taaaagcatt taatacaacc
      121 ccacaaaaac acaaaagtct tgtcaaagag ttacttagtg cgctgggtga tactgtaggg
      181 gaaggcatta tagaaactga cgctaaactt cttgcagagg ctatttataa taaagattac
      241 aagattgaac acagcaccaa gttttatgag ggaatgtcgc aatatgccgt taaagtgcta
      301 caaaccagtg atgtaattgc cgcgctgaat cctgtaaaag gtgacgatgt tgatgcgctt
      361 aaagtcattg atgccattac ccacaatgag ttttttcaag aaattttttc aacagtcatt
      421 caaaaagcag cctcaagtgc ggcaaaagcc atgtttaaag gaggattccg taaaaaagca
      481 gcacagttta atgtggcatc aaaggcggct tcactgacgt ggggtattgg cacaaaactg
      541 ttacctatga tgtgggatag ctgtaccacc tccaaaaaat tatccatagg gattgttaat
      601 ggcaaaatca gccaatatca agcacccaaa gttgacctgt ggatagaaga cctaacaaca
      661 ggaaagcctg tatattggta taacaatatg ggtgaatcct tgagtgcatc gggtgatacc
      721 ctgaccgcca ttcaaggccg taaatatcgt attcatctta aatcaaagca agcaggcata
      781 ttagactaca gcctcgataa taataaatca gatgccgtac ataaaagttg gatacgtacg
      841 cctgttaaag tgaatggatt gtttattaga cgtacgacgg tagaaaacga aaaccaaatt
      901 ttagcgcata aacaactttg cgctcgtcgt gctttgcaag atggcaatac catggtttat
      961 ggccttaatc aatttacaac gttgtcaggc tttgcttgtg ttgaggggca agttggcacg
     1021 ggtgatgccc aattattttc accgccgttt aatcaaacac aaggttttgt gcaacacaat
     1081 ggcaacgtaa actatgcaac cacaacctat cgtgaaggct taggttttag ttcatggcaa
     1141 gagttgacct ctggcgcaac caaaaattta ggttttggtg gtgaagtatc tgacaccttt
     1201 gattttacag aagataaatt aactaaatcc ttatttattg aaattgaggg ttttaatgat
     1261 gaaattcagc aaggggagga tgctaaacag atttttgata ttcgcgttgt taataatgcc
     1321 gatgcagtga ttagcagcaa tgccaaaccc agtgcagcca atggtcctct ttatgttgga
     1381 gatgcaatta cagcaaaagt gactcaaata gttggcaaag tggtgagtgt attttggcag
     1441 ttgattaaca gtttgggcga agtggtcaag agcatgtcgg cagttgttgc agagagtgct
     1501 tcatttgcaa ccgacaacct gtcgggtggc acgtatgaga taaaagcaga gttacgagac
     1561 gaaaatgcac agcctattgg cgtggtgagt caaagcatac ccttgctaca aaccgatttt
     1621 aaatctgtca atgtgcaaag tatgcctgtg ggtgtgcctg tggccattac tgtgtatggc
     1681 aaagacttac cgagtacaac ggtttttgcg gtagagggtg ttttgtgtga gcagccactg
     1741 attgatggcg tttctgtcac gcaaatatgt accgcctctg aagtcaaaaa taatgttaaa
     1801 ttgacggtca agcgtaaaac acaagtcgat gaaggcagtc gtatttttag tattaacatt
     1861 acgccaaagt cagtagcagg tcaatttgtt gtgcctgcca ccagcacagg tgtggagttt
     1921 attccaccaa tagacaacac gcaaagggtg agcgagttgg ggctggtagt gggctatggt
     1981 gcaagccgcc gcaatatgac gtgttcattt accagcactg gcaacacaaa aacctacaaa
     2041 gactacccag acgtagatgc taatggctac agcaccgata ttttaccgtt attgaacggc
     2101 tttgctaata tggcattatt agccaccaaa caacaaggtt caaacacaca aactttgtta
     2161 attggcaata gccaaaccgt gagtattgcc gcaggtcaaa cgcttaactt ttatgttaat
     2221 gatgataact acggcgataa cgaaggcgca ttaaccgtca attatcaatg cactcaatta
     2281 accgccacca ttcaaccctc taccacaacg cctgttgcac aatctcccat taccattggc
     2341 tttgtagata ccgtcattgc caatgtaaaa gatgtggtat gggcggtgag tgatgccgtg
     2401 agtgaaattt ttgtgagtgt acgcaatggc ttaagtgagt tatttgacta cagcttttct
     2461 aaatcaggag actacaaggt aacagccacc gttagagatg ccaacaatca aaccatagca
     2521 accagtagta cacaactcac cgtcacagcc gctcccccca ccgcctcctt actcggctca
     2581 gccaccacag gcatcgttgg ccaacccgtt gtatttgtac ttaacaacag caaagcctta
     2641 gagggcaaaa tttgctcata cctctttgca ggtggtggac aaatcggtga tagtggcggt
     2701 ggtgattgca gcaatgccag ccaagtctta gtgctaccct ctattaccga gctttacacc
     2761 accgcaggca catacacagc cacactcacc gtgaccgaca gccaaggcaa aacagccacc
     2821 acaagccata taataaccgt gaccgaagcc acccaaacag cctctactgg cctattaaac
     2881 gacacaggca ttacccaatg cagtgaccaa agcaccctgt ttgccgactg caccgcagcc
     2941 agcatgggtg gttggtttgg cttaaaccaa gacggcgaaa caggccgcga cttgttagcc
     3001 gttaaaggca cattagccaa aacaggtgca ggtgacgcag gttttgactt tacaaaaatt
     3061 agcgcaacag gcttaccgct acccgccagt gct
//
LOCUS       DAOQMB010000271         3062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_17818_length_3062_cov_10.363153, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000271 DAOQMB010000000
VERSION     DAOQMB010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17818_length_3062_cov_10.363153"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1858
                     /locus_tag="PLY05_08655"
     CDS             <1..1858
                     /locus_tag="PLY05_08655"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNC03409.1"
                     /translation="AFSGLRVLLTAISWPQLDNNPAELASVFLRGLLFDGVVAVYVYG
                     FFAVFGTLIPEKWRKSQWGHKATLVIASIYLFALGFVIVAEWFFWQEFNARFNFIAVD
                     YLVYRREVTGNISESYPVVPILSMVALVATSIAYLLRHRIQQSINHPLNRKHRLLALV
                     TAILLMLGVGQLRGNDVPQLSNNMYIEELAHNGVFQFFYAFRHNHLDYPTFYQTITDL
                     QKASHLLKTQVVLAHERLLYPHELFDVTRQVNVPEVERRLNVMLITVESLSADYMAAF
                     GNQENLTPHLDALSKQGLFFSQYYATGNRTVRGLEALTLGIPPTAGSAVVRRPHNDGL
                     FSLASVLNKKAYDSKFIYGGYGYFDNMNTFFVGNGYQVIDRTDFKEDEQGFANVWGVA
                     DEYLYLRTLREADKSYAAKKPFFSIVMTTSNHRPYTFPQGRINAPQKKWSSAVKYTDW
                     AIANFIKQAKQKPWFNDTIFVISADHCSGSAGKTAVPVERYHIPLIIYAPQHITPKQI
                     DQVASQMDVPPTILGLLNMDYVSRFVGRDILRTPKEEQRALIATYQRLGLYKNDDLVI
                     LSPQKHVEIHRQPLATDQVLRAEANPELVQEAISYYQGTDYMLKHGLLKAVP"
     gene            2029..>3062
                     /gene="carA"
                     /locus_tag="PLY05_08660"
     CDS             2029..>3062
                     /gene="carA"
                     /locus_tag="PLY05_08660"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNC03410.1"
                     /translation="MSKPALLALADGSIFRGTAIGADGSTVGEVVFNTALTGYQEILT
                     DPSYCQQIVTLTYPHIGNTGINNEDEEAAKLWAAGLIIRDLPLVASSFRSQETLSDYL
                     KRHGIVGIADIDTRRLTRILREKGAQNGCILAGDNISEEAALAAARGFGGLKGADLAK
                     VVTVTDNYLWTEGSWQLGQGHVTPKEPAKFKVVAYDFGVKRNILRMLVDRGCDVTVVP
                     AQTPADVVLAMNPDGVFLSNGPGDPEPCTYAIEAIKQFLTTDIPVFGICLGHQLLALA
                     SGAKTLKMKFGHHGANHPVQDLATGRVMITSQNHGFAVDEPSLPANLRATHKSLFDGS
                     LQGIHRTDKPA"
BASE COUNT          824 a          573 c          674 g          991 t
ORIGIN      
        1 ggcttttagt ggcttgcgtg tgttattaac ggctatttct tggccacagt tagataacaa
       61 cccagctgag ttggcgagcg tatttttacg tggtttattg tttgatggtg ttgttgctgt
      121 ctatgtgtat ggtttttttg ctgtttttgg cacattgata cctgaaaaat ggcgcaagag
      181 ccaatggggg cataaagcaa ccctagtcat cgccagtatt tatctatttg ctttaggctt
      241 tgttattgtt gcagagtggt ttttttggca agagtttaat gctcgtttca attttattgc
      301 tgttgattat ttagtctatc gtcgagaagt gacgggtaat atttcggagt cttatcccgt
      361 tgtacctatt ctgtcgatgg tggctttggt tgctaccagt attgcttatt tattgcgtca
      421 ccgtatacaa caaagtatta accatccttt aaatcgtaaa caccgtttat tggccttagt
      481 cactgctatt ttattaatgc tcggtgtagg acagttacgt ggcaatgatg tgccacaatt
      541 atcgaataat atgtacattg aagagttggc acataatggt gtttttcagt ttttttatgc
      601 ctttaggcat aatcacctag attatccaac tttttatcaa accataaccg acttacaaaa
      661 agcctcccac ttattaaaaa cgcaagtggt cttagctcat gagcgtttgt tatatcctca
      721 tgaattgttt gatgtcaccc gtcaggttaa tgtgccagaa gttgaacgcc gtttaaacgt
      781 gatgctaatt acggtcgaaa gtttaagtgc cgattatatg gcagcatttg gtaatcaaga
      841 aaacttaacc ccacatttag atgcactcag caaacaaggc ctatttttta gccaatacta
      901 tgcaacaggc aatcgtaccg tacgcggttt agaagcctta actttaggta ttccacccac
      961 cgcaggcagt gctgtggtgc ggcggccaca taatgatggt ttgttttctt tagcttctgt
     1021 gctgaataaa aaagcttatg acagtaaatt tatttatgga gggtatggct actttgacaa
     1081 catgaatacc ttttttgtgg gtaatggtta tcaggtcatt gaccgtacag actttaaaga
     1141 agatgaacaa ggttttgcta atgtgtgggg agtcgctgac gaatacttat atttacgcac
     1201 tttgcgtgaa gcggataaaa gttatgccgc gaaaaagcct tttttttcta ttgtcatgac
     1261 cacctctaat catcggcctt atacctttcc tcaaggccgt attaatgcac cacaaaaaaa
     1321 gtggagtagt gcagttaaat ataccgattg ggcgattgct aattttatta agcaggccaa
     1381 acaaaagccg tggtttaacg atactatttt tgtgattagt gccgaccatt gttcaggcag
     1441 tgcaggtaaa acagctgtac ctgtagagcg ttatcatatt cctttaatta tttatgctcc
     1501 acaacatatc accccaaaac aaattgacca agtcgctagt cagatggatg taccaccaac
     1561 cattttgggt ttattaaata tggattatgt cagccgtttt gttggtcgtg atattttgcg
     1621 tacgcccaaa gaagaacagc gagcattaat tgcgacctat cagcgtttgg gtttgtataa
     1681 aaatgatgat ttagtcattt tatcaccaca aaaacatgtt gaaattcatc gtcaaccttt
     1741 ggcaaccgat caagtgttac gtgcggaggc aaatcctgag ttggtgcaag aagcaattag
     1801 ttattatcaa ggtacagatt atatgttaaa gcatggactg ttgaaggcag tgccttaatt
     1861 ttaaaattgc aaacaaaatt ggactttttg cttggttttt cgtaaaatcg cgcaaatttt
     1921 ttctaagcga ggcgaatcag tcagcgggat gacaaaatca ccgttgtatc tagttctgtc
     1981 tcgctttttt gtaagtgtac aatcgcaaag tttagcgcgg aggttcgttt gagtaagccc
     2041 gcccttttag ccctagcaga tggcagtatt tttcggggaa ctgctattgg tgctgacggt
     2101 agcactgttg gggaagttgt ttttaatacg gccttaacag gttatcaaga aatcctcacc
     2161 gacccttctt attgtcaaca aatcgttacc ttaacctatc cgcatattgg taatacaggt
     2221 atcaataacg aagatgaaga agctgcaaaa ttatgggcag caggtctaat tattcgtgat
     2281 ttgcctttgg ttgccagtag cttccgtagt caagaaacct tgtctgacta cttaaaacgt
     2341 catggtattg tgggtattgc tgatattgac actcgtcgtt taacccgtat tttgcgtgaa
     2401 aaaggcgcac aaaacggttg tatcttagca ggcgacaata tcagtgaaga agctgccttg
     2461 gctgctgcac gcggttttgg tggtttaaaa ggtgctgact tagccaaagt cgtgaccgtg
     2521 accgataatt acttgtggac agaaggttct tggcaattag gtcaaggcca cgtaacaccc
     2581 aaagagccag ccaaatttaa agtggttgct tatgactttg gtgtaaaacg caatatttta
     2641 agaatgttgg ttgaccgtgg ttgtgatgtg actgttgtac ctgcacaaac ccctgcggat
     2701 gttgtgttag ccatgaatcc tgatggtgtg tttttgtcta atggtcctgg tgatcctgag
     2761 ccatgtactt atgccattga agccattaaa caattcttaa ccacagatat tcctgtgttt
     2821 ggtatttgtt tgggtcatca attattggcc ttggcttctg gtgcaaaaac cttaaaaatg
     2881 aaatttggcc atcatggtgc gaatcatcct gtgcaagatt tagcaacagg tcgggtaatg
     2941 attaccagcc aaaatcatgg ttttgcggtg gatgaaccaa gtttgccagc aaacttgcgt
     3001 gcgacacaca aatccttgtt tgatggttct ttacaaggta ttcatcgtac cgataaacct
     3061 gc
//
LOCUS       DAOQMB010000272         3045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18012_length_3045_cov_9.020401, whole genome shotgun sequence.
ACCESSION   DAOQMB010000272 DAOQMB010000000
VERSION     DAOQMB010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18012_length_3045_cov_9.020401"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..693)
                     /gene="lolD"
                     /locus_tag="PLY05_08665"
     CDS             complement(13..693)
                     /gene="lolD"
                     /locus_tag="PLY05_08665"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004861696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HNC03411.1"
                     /translation="MSKIILEAQGLCKSFKEEGMQTVDVLKNVSLTIQRAEFVAIVGS
                     SGSGKSTLLHILGGLDVPTQGKVTVLGQNLSGLNEVKRGELRNRHLGFVYQFHHLLSE
                     FTALENVCMPLLLRQEATVKQAEQSAKTLLERVGLAHRIHHKPAELSGGERQRVALAR
                     ALVTKPDLVLADEPTGNLDHHTALEVQNLFKELKSELGMSLIIVTHDLALAQSADRIL
                     TMQSGELV"
     gene            complement(686..1921)
                     /locus_tag="PLY05_08670"
     CDS             complement(686..1921)
                     /locus_tag="PLY05_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNC03412.1"
                     /translation="MFKPVSLLIGLRYTRAKRRNHFISFISLTSIIGLVLGVAVLITV
                     LSVFNGFDRELKNRILGMVPHATISSQQPFHDWPKLAEYAKHNPEVLAVAPFSQLQGM
                     ITANGQVTGALISGIDPVYEKQVSIIHHHMAQGHLDSLQANTFGIVLGEGLANNLGVQ
                     LGDKLTLVLPEAAVSPAGVIPRFKRFTVVGIFKVGADVDGMLAYIHIADAGKLLRIGD
                     QVQGVRLKLHDIFQSRPVLLDLLSQLPSYFEARDWTQTHGNLFSAIQMEKAMMGLLLL
                     LIVAVAAFNIVSSLVMVVTDKKADIAILRTLGASPQTITRIFMVQGAVIGLVGTLGGV
                     LLGVTLALNISDFISWVEQVTGASLFDAYFINYLPSELDWNDVAWVSFMSALLSFLAT
                     IYPARRAAKVQPAEALRYE"
     gene            2161..2760
                     /locus_tag="PLY05_08675"
     CDS             2161..2760
                     /locus_tag="PLY05_08675"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC03413.1"
                     /translation="MTKANQDNPFERRLLNRVDSTLSLHYQPISSADSLKDPYDSNFS
                     LPRYFTLFAEIEQVDSVQRSQMQLLERENERLHGLFQAVNLKIELLTGAIYDGLAKLL
                     SPIPQRVNLSESGLSFHATEAITPGTHLHLAISHLEHHYHIAATARVVYCEDEDLEGF
                     RTGVYFININPSDRARLAQDIARKQQENEALQPFANPEE"
     gene            complement(2838..>3045)
                     /locus_tag="PLY05_08680"
     CDS             complement(2838..>3045)
                     /locus_tag="PLY05_08680"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656800.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNC03414.1"
                     /translation="GESFLDCINENALTVVQAIVEPSLATAQGEERFQFEREGYFVAD
                     LKDCQAGKLVFNRTVGLRDSWGGA"
BASE COUNT         1001 a          697 c          508 g          839 t
ORIGIN      
        1 aagggcaacc aattagacca actcaccact ttgcatagtt aaaatacgat ctgctgactg
       61 tgctaaggct aaatcatgag tgacaataat caaactcata cctaactcgc ttttcagctc
      121 tttaaataaa ttctgcactt ctaaagcggt atgatgatct aaattacctg ttggctcatc
      181 ggctaatact aaatcgggtt tagtgactaa tgcacgagct aaagccacac gttgccgttc
      241 accacctgat aactccgcag gtttatgatg aatacgatgc gccaaaccca ctctttctaa
      301 tagcgttttt gccgattgtt cggcttgttt gactgtggct tcttgacgca acaataaagg
      361 catacaaaca ttttctaagg cggtaaactc agacaataag tgatgaaact gatacacaaa
      421 gcctaaatga cgattacgca actcgcctcg tttcacttca tttaaaccac taagattttg
      481 tcctaaaaca gtgactttac cttgagttgg cacatccaag ccccccaaaa tatgcaacaa
      541 cgtgctttta cccgatcctg agctaccaac aatcgccaca aactccgcac gctgaatagt
      601 caaactgacg ttttttaaca catcaaccgt ttgcataccc tcttctttaa aagatttaca
      661 taagccttga gcttctaaaa taattttact cataacgtaa agcctcggca ggttgaactt
      721 tagccgcacg tcgtgctgga taaattgtcg ctaaaaagct tagtaatgcc gacataaaac
      781 tcacccatgc cacatcgttc caatccaatt ctgagggcaa ataattaata aagtaggcat
      841 caaacaaaga agcacctgtc acttgctcaa cccaagaaat aaaatcactg atatttagag
      901 ccaatgttac gcccaataac acaccaccta atgttcctac taagccaatc actgcgcctt
      961 gcaccataaa aatacgcgta atggtttgtg gtgacgcgcc taatgtccgc aaaatagcaa
     1021 tatcggcttt tttatcggtg accaccatca ctaaacttga aacaatatta aaggcagcca
     1081 ccgcaacaat taacaacaac aacaagccca tcatggcttt ttccatttga atagcactaa
     1141 acaaatttcc atgtgtttga gtccaatctc gtgcttcaaa ataactgggt aattggctta
     1201 ataaatctaa taacacagga cgcgattgaa aaatatcatg gagttttaaa cgtacacctt
     1261 gtacctgatc gccaattctt aataacttgc ctgcatcagc aatgtgaata taagccagca
     1321 tgccatcaac atctgcacca accttaaaaa tccccacaac agtaaaacgt ttaaaacgtg
     1381 gaatcacccc tgctggcgat acggctgctt ctggcaatac caaggttaat ttatcaccta
     1441 attgcacacc taagttattt gctaatcctt cacccaacac gataccaaaa gtattggctt
     1501 gtaaactgtc caaatgtccc tgtgccatgt gatgatgaat aatggatacc tgcttttcgt
     1561 aaacaggatc aatacccgaa attaacgcgc ccgtcacttg accattagcc gttatcatcc
     1621 cttgtagttg gctaaagggt gcaaccgcaa gcacttcggg attatgttta gcgtattcgg
     1681 ctaatttagg ccaatcatga aaaggttgtt gtgagctaat cgtggcatga ggaaccatac
     1741 ccaaaatacg attttttaat tcacgatcaa atccattaaa taccgataat accgtgatta
     1801 aaaccgccac ccccaacact aaaccaataa tggaagtcaa agaaataaaa gaaataaaat
     1861 gatttcgacg cttggctcgg gtataacgta aaccaattaa taatgaaaca ggcttgaaca
     1921 tgctaaaaat ttaaccatac tattaataac ttatttaagt tataacttag cttaagtatg
     1981 ctgtttaaca acgccacttt gactaagcta taagttaaat acgccacatt atcaataaat
     2041 taacctaact tactgtgcta ataatcttta ctacaacacc ttatttggtg taaaaagcat
     2101 aacaaattaa ataaaacatc tgtcataaat atggcaagta tgccttaaga gtggaacatc
     2161 atgactaaag ccaatcaaga taatcctttt gaaagacgct tacttaatcg tgtggattcc
     2221 acactatctc tgcattatca accaattagt agtgcagatt cgttaaaaga tccttatgac
     2281 agtaattttt cgttaccgcg ttattttacg ctatttgctg agattgagca ggtagatagt
     2341 gttcaacgta gtcaaatgca attattagaa cgtgagaacg agcgtttaca tggcttattt
     2401 caggccgtca acctcaaaat tgagctatta acaggcgcga tttatgatgg cctagctaag
     2461 ttattatccc ctattcccca gcgtgttaat ttaagcgaaa gcggtttatc ttttcatgcc
     2521 accgaagcga ttacaccggg tacacaccta catttagcca tcagtcatct tgagcatcat
     2581 tatcatattg ccgccacagc acgtgtggtt tattgtgaag atgaagattt agaaggcttt
     2641 agaacaggcg tgtattttat taatataaat cccagtgatc gtgcgcgttt agctcaagat
     2701 attgcccgta aacaacaaga aaatgaagca ttacaacctt ttgccaaccc tgaagaataa
     2761 ccgtagggat aacccttgtg gttatcctgt tgaatttgaa ataaaagggt aagcacaagg
     2821 cttaccccta caaaaaatta tgcgccaccc caagagtcgc gtaagcctac agtgcggtta
     2881 aaaaccaact tacctgcttg gcaatctttt aaatcagcaa caaaatagcc ttcacgctca
     2941 aattgaaaac gttcttcgcc ttgcgcggtt gccaaactag gctcaacgat ggcctgcaca
     3001 acggttaaag cattctcgtt aatacaatct aaaaagctct cgcct
//
LOCUS       DAOQMB010000273         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18044_length_3042_cov_14.782056, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000273 DAOQMB010000000
VERSION     DAOQMB010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18044_length_3042_cov_14.782056"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLY05_08685"
     CDS             complement(<1..218)
                     /locus_tag="PLY05_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC03415.1"
                     /translation="MSYNFDQILNHHQQNSIKHNANRQFFGTDDVLPLWVADMDFAVA
                     PAISQAIQQRSQYPIFGYTFYPETMYQA"
     gene            complement(215..2326)
                     /gene="ovoA"
                     /locus_tag="PLY05_08690"
     CDS             complement(215..2326)
                     /gene="ovoA"
                     /locus_tag="PLY05_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011182050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-histidylcysteine sulfoxide synthase"
                     /protein_id="HNC03416.1"
                     /translation="MDTPLFSRTPLLTGHDVESTRQEMLLYFLDTFNRYESLFECLAN
                     EEAYYKKPISLRHPLIFYLGHTATFFVNKLLLVHLIDQRINPRFESMFAVGVDEMSWD
                     DLNDAHYDWPSVNEVKAYRQQVRTLVSQLIQEAPLTLPINWQNPWWAIIMGIEHERIH
                     LETSSVLIRQHQLAYVKPHPAWTAHHQTAQAPNNDLVTIPAGQVTLGKQKDDAYYGWD
                     NEYGQHSADVASFQASRYLVSNQEFLAFVADNGYQQAAYWSEEGWAWREFAQAQYPTF
                     WRKQGHDWQLRLMTEEIPMPWDWPVEVNYHEAKAFCQWLSQQQQQQIRLPSEDEWQRL
                     YQYAQLDHPTAGQRCQANLHLDYAASACPVTQFQHGELFDVVGNVWQWTETPTYPFTG
                     FDVHPIYDDFTTPTFDDRHNLIKGGSWISCGNESHHNSRYAFRRHFFQHAGFRYIVAD
                     HQPVSVSSRYETDKHMSEYAEFHYGDNYFGVANFSQSLAQLAIAQMGNRPARHALDLG
                     CASGRASFELAKHFQQVTGIDFSARFIQQGVQLAEQGVLRYAICEEGELVSYKERQLR
                     DVNLHHVRHKVSFYQGDACNLKAQFTDYDLIFAANLIDRLYDPAKFLSTIHQRLTMSG
                     VLMIASPYTWLEEHTPRANWLGGFKKDGESFSTFDGLTAILSKHFRLMTEPQAVPFVI
                     RETKRKFQHSLSEVSFWERIQ"
     gene            2433..2921
                     /locus_tag="PLY05_08695"
     CDS             2433..2921
                     /locus_tag="PLY05_08695"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HNC03417.1"
                     /translation="MLQGFPPIASAHSQVLILGSMPSVASLQAQQYYAHPRNAFWPIQ
                     QALWGIADQASYAQRVQILQAKGVAVWDVLASCKREGSLDRAIEGDSVVVNDFKSFFA
                     EYQQMKAIFFNGATAENYFKKYVLLKPSLVFQRLPSTSPAHASLSFEQKLAKWQQIKH
                     YV"
BASE COUNT          857 a          613 c          700 g          872 t
ORIGIN      
        1 aacgcctgat acatcgtttc gggataaaag gtataaccaa aaatcggata ttggctgcgc
       61 tgttgaatcg cttggctaat cgcaggtgca acggcaaaat ccatatctgc cacccacaac
      121 ggcaacacat cgtctgtgcc aaaaaattgg cgattggcat tgtgcttaat gctgttttgc
      181 tgatggtggt ttaagatttg gtcaaaatta taactcattg aatacgttcc caaaaactca
      241 cttctgataa gctatgttga aatttacgtt tggtttctct aatcacaaac ggcaccgctt
      301 gcggttcggt cattaatcta aagtgtttgc taagtatcgc ggttaaacca tcaaaggtgc
      361 taaaactttc gccatctttt ttgaagccac ctagccagtt ggcgcgtggt gtatgttctt
      421 ctaaccatgt atagggtgaa gcaatcatta ataccccact catggttaag cgttgatgaa
      481 tcgtgcttaa aaatttggcg gggtcatata aacggtcaat taaattggcg gcaaaaatga
      541 ggtcatagtc ggtaaattgg gctttgagat tacaggcatc accttgataa aaactgactt
      601 tatggcgcac atgatgcaag tttacatcgc gtaattgtcg ctctttataa ctgaccaatt
      661 cgccttcttc acatatcgca taacgtaaaa ccccttgttc ggccaattgt acgccttgtt
      721 gaataaaacg tgccgaaaaa tcaatgccag tgacttgctg aaaatgtttg gctaattcaa
      781 aactggcgcg accagaggcg caacctaaat caagggcatg gcgagcaggg cgattgccca
      841 tttgtgcaat ggccagttgg gcaaggcttt ggctaaagtt ggcaacacca aaatagttat
      901 cgccataatg aaactcggca tattcagaca tgtgtttgtc ggtttcgtaa cgtgagctga
      961 cggatactgg ttgatggtca gccacaatgt atctaaagcc tgcgtgttga aaaaagtgac
     1021 ggcggaaagc ataacgtgag ttgtgatggc tttcgttgcc acaagaaatc catgagccgc
     1081 ctttgattag gttatggcgg tcgtcaaagg tgggggtagt aaagtcatca taaatcggat
     1141 gtacatcaaa ccctgtaaac ggataagtgg gcgtttctgt ccattgccaa acattaccca
     1201 cgacatcaaa caattcgcca tgctgaaact gcgtgacagg acaagccgac gctgcataat
     1261 ctaaatgtaa gttcgcctga caacgttgac cggcagtggg gtggtcaagt tgggcatatt
     1321 ggtacaggcg ttgccattca tcttcactgg gtaaacgaat ttgttgttgc tgttgttggc
     1381 ttaaccactg acaaaaggct ttggcttcat gataattcac ttcgacaggc caatcccaag
     1441 gcatggggat ttcttcggtc attaagcgta attgccagtc gtgaccttgt ttacgccaaa
     1501 aagtcggata ttgtgcttgg gcaaactcac gccatgccca accttcttcg ctccaataag
     1561 ccgcttgttg ataaccattg tcggcaacaa aggctaaaaa ctcctgatta ctgacgagat
     1621 aacgactggc ttgaaagcta gcgacatcgg cactgtgttg gccatattca ttgtcccaac
     1681 cataataggc atcgtctttt tgtttgccca acgtgacttg gcctgctgga atggtgacta
     1741 aatcattgtt gggtgcttga gctgtttggt ggtgtgctgt ccatgcagga tgtggtttaa
     1801 cataagccag ttgatgttgg cgaattaata ccgaggaggt ttcgagatga atccgttcat
     1861 gctctatccc catgatgatt gcccaccacg gattttgcca attaatgggt aaggtgagtg
     1921 gcgcttcttg aatcagttgg ctaactaatg tgcgtacctg ttgacgatag gctttaacct
     1981 cattaacact tggccagtca taatgggcat cgttaaggtc gtcccaactc atttcatcca
     2041 caccaacggc aaacattgac tcaaaacgtg ggttaatgcg ttggtcaatt aaatgcacta
     2101 ataacagttt gttgacaaaa aaagtcgcgg tatggcctaa ataaaaaatt aagggatgac
     2161 gtaggctaat cggttttttg tagtaggctt cttcgttggc caagcactca aacaaggatt
     2221 cataacggtt aaacgtgtct aagaagtaaa gcaacatttc ttggcgtgtg ctttctacat
     2281 cgtgacctgt taataagggt gtgcgtgaga ataaaggcgt atccataatg ttctcaattt
     2341 ttcttttgaa taaatacttt gtaatagaaa gatattttat tgtctagtaa acaaggtata
     2401 tacctaatat ttggatagat aagagcagat aaatgttaca aggttttcca ccaattgcga
     2461 gtgcccatag tcaagtgttg attttgggca gtatgcccag tgtcgcttcc ttacaagcac
     2521 aacaatatta tgcccatcca cgtaatgcct tttggccaat tcagcaagca ttatggggta
     2581 tagccgatca agcgtcttat gcgcaacgtg tgcaaatttt gcaagcaaaa ggcgtggcgg
     2641 tgtgggatgt acttgcctct tgtaaacgtg aaggcagttt ggatcgtgcg attgaaggtg
     2701 attcggtagt agttaatgat tttaaaagct tttttgctga atatcagcag atgaaagcga
     2761 tattttttaa tggtgcaacc gccgaaaatt attttaaaaa atatgtgttg cttaagccat
     2821 cattggtttt tcagcgtttg ccatcaacca gtcctgctca tgcgagtcta tcatttgagc
     2881 aaaaactggc taaatggcaa cagattaagc attatgttta ggccataacc aatcactaaa
     2941 cacctgacta cttttagccg cgatgagttg tgaacagata aataaaggag catctacagg
     3001 gcgaatgcct gcaaaagtat cagttaaggt gcgcgcaaca gt
//
LOCUS       DAOQMB010000274         3040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18066_length_3040_cov_15.764489, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000274 DAOQMB010000000
VERSION     DAOQMB010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18066_length_3040_cov_15.764489"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1597
                     /locus_tag="PLY05_08700"
     CDS             <1..1597
                     /locus_tag="PLY05_08700"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC03418.1"
                     /translation="HHLLNEAIQHSMNAQDYTLAAQLMEDNSSQLFSLGRITTAVTWA
                     YQLPSSILANCPKLSMLCAWAGLVMDNLSEIDRHLASIHQYLSNNAIDTNKQHRLLGQ
                     IALIRGCQYCLTSDIERAEASVMEALNYLSPKQVLYSGASVCLGFCYYVQGKIDKAQR
                     LFMANAHISQAKYNLMIPIFAVLGLGRCYLLRGELEAAEQIYNRALAECIAFGWQDVP
                     VCGMLHLGLGELAYESNQLVEAILHLEKGVAMTRIGQMQFFNAWGRVLLAQAQIALKQ
                     TVVLSAIEEAELIRYSERFLIEIPPLSASLVQLWLQQQRFDCVQQWLNNAQLPVKGEL
                     VFERWAEYLVLCRFFIKQQRYQETAYILDKLYDYAENQQHRRLLVEVLLIQSIMQYQQ
                     HLLSKAADSLQKAIISTHDSKFLRLFAHEDHLLETLLPLISKSLIHHPLMCDIMALLA
                     PHTETPAVTDNTSLSSYLVLSKKERLVARKMISGASSQEIAEMLCVSLSTIKTHTQNI
                     YTKLGVNKRPQAIEMLLRLNLAT"
     gene            complement(2065..2451)
                     /gene="gloA"
                     /locus_tag="PLY05_08705"
     CDS             complement(2065..2451)
                     /gene="gloA"
                     /locus_tag="PLY05_08705"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005176820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HNC03419.1"
                     /translation="MRLLHTMLRVVDLEKSLAFYTQVLGMQLLRRKDYPEGRFTLAFV
                     GYGAEHEGAVIELTHNWDTTSYELGNAYGHIALEVDDVYQACERIKAAGGKVTREAGP
                     MKHGTTILAFVEDPDGYKIELLGSKS"
     gene            complement(2465..>3040)
                     /locus_tag="PLY05_08710"
     CDS             complement(2465..>3040)
                     /locus_tag="PLY05_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03420.1"
                     /translation="LLTAMRMPPSEEELQQAGLSLVIQKPTSANSLRDALLKLLGCQE
                     QYRSSSEKRQDIGIIKHILQGKNVLIAEDNQVNQMVIVGMLKRLGMTADIAKNGEEAL
                     ALYQQNYERYQLVLMDCEMPEIDGYQATQQIRAFEHEYNLKSVTIVALTAHAMRDQQQ
                     RCVQAGMDDFLTKPLAFERLKQLLMQIFWQK"
BASE COUNT          977 a          621 c          517 g          925 t
ORIGIN      
        1 acatcattta ttaaacgaag ccattcaaca tagtatgaat gctcaagatt acacactagc
       61 tgcacagtta atggaagata atagctcaca actattttct ttaggccgaa ttactactgc
      121 tgtcacttgg gcataccaat taccttctag catcttagcc aactgtccca agttatctat
      181 gttatgtgct tgggcaggtt tggtgatgga caatctctca gaaatagatc gtcacttagc
      241 ctctattcat caatacctca gtaataatgc tattgatacc aacaaacaac atcgcttgtt
      301 aggtcaaata gccttaattc gtggttgcca atattgccta accagtgata ttgaacgtgc
      361 cgaagcctct gtgatggaag ctctaaatta tttgtcaccc aaacaagtgt tatatagtgg
      421 cgcatccgtt tgtttgggct tttgttatta tgtacaaggc aaaattgaca aagctcaacg
      481 attgtttatg gccaatgccc atattagcca agccaaatat aacttaatga ttcctatttt
      541 tgccgtctta ggtttaggac gttgttattt attacgtggc gaactagaag cagctgaaca
      601 aatttataat cgcgctttag cagaatgtat cgcttttggc tggcaagatg tgccagtctg
      661 tggaatgttg catttaggat taggtgaatt ggcttacgaa agtaatcaat tagttgaagc
      721 gatattgcat ttagaaaaag gcgttgccat gacacgcatt ggtcaaatgc agttttttaa
      781 tgcttgggga cgtgtgttat tagcacaagc tcaaattgcc ctaaaacaaa ccgtcgtttt
      841 atcagctatc gaagaagcag agctaattcg ttattccgaa cgctttttaa ttgaaattcc
      901 gccactctct gcgagcttag tacaactttg gttgcaacaa cagcgttttg attgcgtaca
      961 acaatggcta aacaatgcgc aattacctgt taagggtgag ttagtttttg agcgttgggc
     1021 agaatactta gtattatgtc gcttttttat taaacaacaa cgctatcaag aaacagctta
     1081 tatattagac aaactatatg actatgcaga aaaccaacaa catcgtcgtt tactggttga
     1141 ggttttgctt attcaatcca tcatgcaata tcaacaacac ttattgagta aagctgccga
     1201 cagtttacaa aaagcgatta tttccactca tgacagtaaa tttttacgtt tgtttgctca
     1261 cgaagaccat ttgttagaga ccttgctacc actgattagc aaaagtctga ttcatcatcc
     1321 tttaatgtgc gatatcatgg ctttacttgc tccccataca gaaacaccag cagttaccga
     1381 taacacatct ttatcttctt atttagtatt aagcaaaaaa gaacgcctag tcgcccgaaa
     1441 aatgattagc ggagcaagca gccaagaaat agccgaaatg ctttgtgtgt ctctaagtac
     1501 cattaaaaca cacacacaaa atatttatac caaacttggt gttaacaaac gtccccaagc
     1561 gatagaaatg ttactgcgct taaatttggc tacatgacgt tacctaaact aaacacttgt
     1621 tacggtaaca tacaaaagct gttacttaga cactgtcaag atgttacctt tttaagaaac
     1681 agttcacata accacagcca tcaaaaaaat taactatttg atatataaac aaaaaatatt
     1741 ttatataaaa gttggcacgc tatctgcaat atatattatg cgtagcaaat aacaacaaat
     1801 agtgcagcgc gtaacaacaa cgacaataaa ccataataat aagatgaaaa gtggtttgtg
     1861 caatgtcagg aaggcaacat cgtacaaata gcttattgag tggccaaaaa aataacaagc
     1921 ctgccactaa acagagtaga taactggcga ataaaaacaa taagcgtcag agcaagcgat
     1981 aaataacaac aataatcgcg gcaaacaata aaaataagag gcaaattagt gcaaacgtgg
     2041 gagtcctaga ctcccacgcc tcttctagct tttagaacct aataattcaa ttttgtaacc
     2101 atctggatct tcaacaaaag ctaaaattgt tgtgccatgc ttcattggcc ctgcttcacg
     2161 tgttactttg ccgcctgctg ctttaatgcg ctcacatgct tgatacacat catcaacttc
     2221 taaagcaata tgtccataag cattgcctaa ttcatatgat gtggtgtccc aattgtgcgt
     2281 taactctatc accgcacctt catgttctgc accatagccc acaaacgcta aagtaaaacg
     2341 tccttcggga taatctttac gtcttaacaa ctgcatccct aaaacttggg tataaaaagc
     2401 taaagatttt tctaaatcaa ccacgcgcaa catggtgtgt aataaacgca tagatatttg
     2461 actcctattt ttgccaaaaa atttgcatta ataattgttt taaacgctca aacgctaaag
     2521 gtttggttaa aaaatcgtcc atgcctgctt gtacacaacg ctgctgttga tctcgcatag
     2581 catgagccgt taatgcaaca atcgtgaccg attttaaatt atattcatgt tcaaaagcac
     2641 gtatttgctg cgtagcttga tagccatcaa tttcgggcat ttcacaatcc attaaaacta
     2701 attgataacg ttcataattt tgttggtata aagccaatgc ttcttcgcca tttttggcaa
     2761 tatctgcggt cattcctaat ctttttagca taccaacaat taccatttga tttacttgat
     2821 tatcctcagc aatcaacaca tttttaccct gtaaaatatg tttaataata cctatatctt
     2881 gacgcttttc actggaagat cgatattgtt cttggcatcc taataatttt aacagcgcgt
     2941 ctctcaagct attcgcagaa gtaggttttt gaataaccaa gctcaaacct gcttgttgta
     3001 attcttcttc actaggcggc attcgcattg ccgttaataa
//
LOCUS       DAOQMB010000275         3024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18249_length_3024_cov_15.098686, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000275 DAOQMB010000000
VERSION     DAOQMB010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18249_length_3024_cov_15.098686"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1887
                     /locus_tag="PLY05_08715"
     CDS             <1..1887
                     /locus_tag="PLY05_08715"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNC03421.1"
                     /translation="SMKFPLFILSSLFISQFSFASNLDTWQQQAKQQNLANHPYWRIL
                     LRYEQQKDSQWQSIVNDKTFFLSAEGQHNSQAELQATLAALASDNITDKAVSCQFPAR
                     VSWLKTQLQISDSQLPKVDCPALQTWLTGINPHQATLVFAADFINNPSSMFGHTLLRI
                     DTAEQTEDTRLLAYAVNYAAQTNTANGLEFAYKGLTGGYAGAFSILPYYEKVKEYNDF
                     ENRDLWEYQLNFTPEEITQGLKHLWEIKRVNFPYYFLSSNCSYQLLGLLEAARPDTYL
                     RKDFPVYAIPTDTLRRVLEEKNILRKLVYRPANGTTLAYHAQQNNPLLNKAAKALAKD
                     VNYSVAHLNSADQARAYEMAYDYLYYLFLAHKVDKDTAPSQLRQLLIKRSQYQIADQR
                     ITPPQPKVDPAQGHGTTRLQFGLSHQQDQDISFAEWRPAYQDLLDNDQGYRRGAGIDF
                     LRLRVGYNHSTQQAKLLNFTLLNIDSLASGNDFIQPLSWSFALGLEQEAIDNQQFSEQ
                     KQHTVSYVRGGAGVASPLHSQDWLCFALAQGHFQAATAIEDGWRVGVGGKLGCRQQTT
                     FGQLLLDVQPMYYSDHQTLTTTTRAAYQWQYSVNQGLRVQLLNQQQKHKDWQSIEASW
                     VRYF"
     gene            2061..2981
                     /locus_tag="PLY05_08720"
     CDS             2061..2981
                     /locus_tag="PLY05_08720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNC03422.1"
                     /translation="MTEPSLLNKIKGSTRLIILTGSTALHSLGIMAHYPLKKLLPTAT
                     SDKFVHPAIFKWAENWTRVNNLFMNRLPDIKFEVDLPAEINYDGKYFIIANHQTWVDI
                     IVLQYAFHKRTTFIRFFTKQELAYIPFLGLALYVMDFPYMKRYSKETLNKHPELQGKD
                     IETTKQSCSQIKRNPFAILNFLEGTRITPKKHQLQQSPYQYLLKPKSGGMAFALKALG
                     SEIKELIDVTVYYPEGAPRFIDFWSGKTKTVIIKGKIRAIPEGFSDGDYENDTEFRER
                     FQTWVNELWQEKDEELAKFNQQLPTNKPQS"
BASE COUNT          970 a          625 c          518 g          911 t
ORIGIN      
        1 tcaatgaaat ttcctctatt tattcttagc tctttattta ttagtcaatt tagttttgcc
       61 tcaaacctag acacatggca gcaacaagcc aaacaacaaa atctagctaa tcatccctac
      121 tggcgcattt tattacgtta tgaacaacaa aaagatagtc aatggcagag cattgttaat
      181 gataaaacct tctttttgag tgctgaaggc caacataata gccaagcaga gctacaagcc
      241 accttagctg ctttagcgtc cgacaacatc acagataaag cagtaagttg ccagtttcct
      301 gctcgggtca gttggttaaa aacacaatta cagatttctg acagccaact cccaaaagtt
      361 gactgccctg ccttgcaaac atggttaaca ggcattaacc ctcaccaagc aactttagtc
      421 tttgctgctg actttattaa taatccttca tccatgtttg gtcatacttt attaagaata
      481 gatactgccg aacaaactga agacactcgt ttattggctt atgctgtcaa ctatgccgca
      541 caaaccaata ctgccaatgg tttagagttt gcttataaag gtttaacagg cggttatgca
      601 ggggcttttt cgattttgcc ctattatgaa aaagtcaaag aatacaatga ttttgaaaat
      661 cgtgatttgt gggaatatca gctcaatttt acacctgaag aaattactca aggtctaaaa
      721 catttgtggg aaattaaacg ggttaatttt ccttattatt ttttatcgtc aaattgctct
      781 tatcagttat taggtttatt agaagcggct cgacctgata cttatttacg caaagacttt
      841 cctgtttatg ccattccaac tgacaccttg cgtcgagtat tagaagaaaa aaacatttta
      901 cgcaaattag tttatcgtcc tgcaaatggc acaacactgg cctatcatgc ccaacaaaat
      961 aacccacttc ttaataaagc cgcaaaagcc cttgctaaag atgttaacta ttctgttgcc
     1021 cacttaaata gtgccgacca agctcgtgct tatgaaatgg cttatgatta tttatattat
     1081 ttatttttgg cgcataaagt cgataaagat accgcaccta gtcaacttcg ccaattatta
     1141 ataaaacgca gccaatatca aatcgctgat caacggataa cgcctcctca gcccaaagtt
     1201 gatcctgcac aaggtcatgg cacaacacgt ttacaatttg gcttgagtca ccagcaagat
     1261 caagacatca gttttgccga atggcggcct gcttatcagg atttattaga caatgatcaa
     1321 ggctatagac gtggtgcagg cattgatttt ttacgtttgc gtgttggtta taaccactcg
     1381 acacaacaag ccaaactgtt aaatttcacc ttgcttaaca ttgactcctt ggcatcaggc
     1441 aacgacttta ttcagccttt atcatggtct tttgccttag gtttagaaca agaggccatt
     1501 gataaccaac aatttagtga gcaaaaacaa cataccgtta gttatgtgcg tggtggcgca
     1561 ggtgtggcta gtcctttaca cagccaagac tggttatgtt ttgctttagc acaaggtcat
     1621 tttcaagcag ctaccgccat tgaagatggt tggcgcgttg gtgtgggagg aaaactcggc
     1681 tgtcgtcaac aaacaacgtt tggtcaatta ttattagatg tacaacccat gtattacagt
     1741 gaccatcaaa cgttgaccac caccacacga gccgcttatc aatggcagta ttcagtaaat
     1801 caaggtttac gagtgcaact gttaaatcag caacaaaaac acaaagactg gcaaagtatt
     1861 gaagcaagtt gggtgcgtta tttttaatca tctaccttta aaacatgata agcgtcatac
     1921 agttaaccaa attttggccg tttataataa ttttacactt gtaaaataat cgaattttgc
     1981 cattagtcaa agctaagttg ttgcagtctt aacaacaagc ccctaacatt accaagtttt
     2041 catatttgga tctgactggc atgactgagc cttcgttgct aaataaaata aaaggtagca
     2101 ctcgtttaat aattttgact ggtagtactg cgctacattc tttaggcatt atggcacact
     2161 acccccttaa aaaattatta ccaacagcca ccagtgataa atttgttcat cctgctattt
     2221 ttaaatgggc agaaaattgg acacgtgtta ataacttatt tatgaatcga ctacctgata
     2281 ttaaattcga agtcgattta cctgcagaaa ttaattacga tggcaaatac tttattattg
     2341 ccaatcatca aacatgggtt gatattattg ttttgcaata cgcttttcac aagcgcacca
     2401 cctttattcg tttttttacc aaacaagaat tagcctacat tcccttttta ggcttagcgt
     2461 tatatgtcat ggattttcct tacatgaagc gttactccaa agaaacatta aacaaacatc
     2521 cagaactaca aggcaaagac attgaaacaa ccaaacaatc ttgtagtcaa attaaacgta
     2581 acccttttgc tatcttaaat tttctagaag gcacacgtat cacccctaaa aaacaccaat
     2641 tacaacaatc gccatatcaa tatctattaa aaccaaaatc aggcggaatg gcttttgccc
     2701 tcaaagcatt aggtagcgaa attaaagagt taattgatgt caccgtatat tatcccgaag
     2761 gagcacctcg ctttattgat ttttggtcag gaaaaaccaa aacagtcatc atcaagggta
     2821 agattcgtgc catccctgaa ggctttagcg atggtgatta tgaaaacgat actgaattta
     2881 gagaacgctt tcaaacttgg gttaatgaat tgtggcaaga aaaagatgaa gaactcgcca
     2941 aatttaatca acaacttcca acaaataagc ctcaaagcta gttagctcgt catttaatca
     3001 tgctagaatt gttcgctttt ttat
//
LOCUS       DAOQMB010000276         2994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18580_length_2994_cov_18.139503, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000276 DAOQMB010000000
VERSION     DAOQMB010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18580_length_2994_cov_18.139503"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..584)
                     /gene="tolR"
                     /locus_tag="PLY05_08725"
     CDS             complement(147..584)
                     /gene="tolR"
                     /locus_tag="PLY05_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HNC03423.1"
                     /translation="MEQSPFQRPKRHLLAEMNVVPYIDVMLVLLVIFMITAPMLTQGV
                     KVELPKVASETIQVNSEEPVVVSIKADGSYWLKQGKDDNQSMALDELTSHLLTAQQQQ
                     PNLQVLINGDTQVPYGEVVKLMAALQKAQIIRVGLLTEPPQDQ"
     gene            complement(587..1255)
                     /gene="tolQ"
                     /locus_tag="PLY05_08730"
     CDS             complement(587..1255)
                     /gene="tolQ"
                     /locus_tag="PLY05_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016140259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HNC03424.1"
                     /translation="MTEQLSVIGLISHASIPVQLVMLSLVAASVYSWTLIAKYRTVHR
                     RAAQDLKTFEEEFWSGADLGSLYQRSQHNTDRQGTEALFYAGFKEFMRLKQKGSPHEF
                     IMEGVTRAMRITLVQEQNRLEGKLPALASIASTAPYVGLFGTVWGIMSSFIGLAKVQQ
                     ATLATVAPGIAEALIATAIGLFAAIPAVLAYNHFMANSDVLNNKYSMFADEFLGILHR
                     EGSY"
     gene            complement(1261..1653)
                     /gene="ybgC"
                     /locus_tag="PLY05_08735"
     CDS             complement(1261..1653)
                     /gene="ybgC"
                     /locus_tag="PLY05_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HNC03425.1"
                     /translation="MTVFSLNIRVYIEDTDAAGIVYYVNYLKFMERARTEYLRHIGFG
                     HYLLTEDYLFVVRKAQIDYKQPAKMDDELRVTAQIIKQGKASLLFEQCIYRQDTLLSR
                     AEIDIACVAKQSLKPCALPTRLTELLTK"
     gene            complement(1653..2681)
                     /gene="ruvB"
                     /locus_tag="PLY05_08740"
     CDS             complement(1653..2681)
                     /gene="ruvB"
                     /locus_tag="PLY05_08740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNC03426.1"
                     /translation="MAHDRIIAPHAQRQEEIIDRAIRPTSLADYIGQPKVREQMEIFI
                     HAAKTRGEALDHTLIFGPPGLGKTTLANIIAREMEGELKSTSGPVLEKAGDLAALLTN
                     LEEGDVLFIDEIHRLSPVIEEILYPAMEDYQLDIVIGEGPGARSIKLDLPPFTLVAAT
                     TRAGLLTSPLRDRFGIVQRLEFYNVVDLSTIVSRSARLLNVPMEEAGAVEIARRSRGT
                     PRIANRLLRRVRDYAEVKSNGVVTGEVACAALNMLDVDHHGFDNLDRRYLLALMEKFN
                     GGPAGVEALAASIAEDSGTLEDVVEPYLIQQNMVMRTARGRIATTQAYLNFGMTMPDS
                     LKTLTGQA"
BASE COUNT          878 a          620 c          655 g          841 t
ORIGIN      
        1 gtcaccaatg tcgcttgaat agcaggtgta ggcggtggca attcgggcac aaaatgttca
       61 gaaaaaatcc acaatgcagc aatgattgcc acatgcaata acaatgattg aacaaaaggg
      121 ctatgcaaat aacgactaat catggattac tgatcttgtg gcggttcagt gaggaggcca
      181 acacggatga tttgcgcttt ttgaagagca gccatcaatt tgaccacttc accataaggc
      241 acttgcgtat caccattaat taatacttgt aagttgggtt gttgctgttg agcggttaat
      301 aaatggctgg ttaactcgtc taaggccatg ctttgattat cgtctttgcc ttgttttagc
      361 caataactac cgtccgcttt aatgctaacc accacaggtt cttcactatt aacttgaatg
      421 gtttcgctgg ccactttggg tagctcaact tttacacctt gcgtgagcat tggcgcggta
      481 atcataaaaa tgactaataa caccaacatt acgtcgatat aaggcacgac gttcatttcg
      541 gctaataaat ggcgtttagg acgttgaaat ggactttgtt ccatgtttaa taactgcctt
      601 cacggtgcaa aatgcctaag aattcatcag caaacatact gtatttgtta tttaatacat
      661 cactattagc cataaaatgg ttgtaggcta ataccgcagg aatggcggca aacaagccaa
      721 tggcggtggc aattaatgct tcagcaatac caggtgcaac cgtggcgagg gtggcttgtt
      781 gaactttagc caagccaata aacgagctca taatacccca aaccgtacca aacaaaccga
      841 cataaggggc agtggaggca atactggcta aagcaggtaa tttaccttca aggcggtttt
      901 gctcttgaac aagggtaata cgcatggcac gagtaacacc ttccataata aattcatggg
      961 gtgagccttt ttgtttaaga cgcataaact ctttaaagcc tgcataaaac aaggcctctg
     1021 tgccttggcg gtcagtatta tgctgactgc gttgatatag gctgcctaaa tctgcacctg
     1081 accaaaactc ttcttcaaat gttttaaggt cttgagcggc acgacgatgt acagtgcgat
     1141 atttggcaat taatgtccat gaatacacag aggcagccac taaggacaac atgactaatt
     1201 gtacaggaat gctggcatgg ctaattaagc caattacaga gagttgttcg gtcataatgc
     1261 ttacttagtc aataattcgg ttaaacgagt gggtaaggca cagggtttta ggctttgctt
     1321 tgcaacacaa gcaatgtcaa tttcagcgcg cgataataac gtatcttgac gatagataca
     1381 ttgctcaaat agcaaagagg ctttgccttg tttaataatt tgagccgtga cacgcaattc
     1441 atcatccatt ttagcgggtt gtttgtagtc aatctgcgct tttcgtacaa caaaaaggta
     1501 atcttctgtt aataaataat ggccaaagcc aatatggcgt agatattcgg tacgggcgcg
     1561 ctccataaat ttaagataat tgacataata aacaatgcca gccgcatcgg tatcttcgat
     1621 ataaacacga atatttaaac taaacacggt cattatgctt ggcctgttaa ggtttttaag
     1681 ctatcaggca tggtcatgcc aaaatttaaa taggcttgag tggtggcgat acgcccacgc
     1741 gcagtacgca tgaccatatt ttgttgaatt aaatagggtt caaccacatc ttcaagtgta
     1801 cctgagtctt cggcaatcga ggcggctaag gcttcaacgc ctgcggggcc accattaaat
     1861 ttttccatta aagctaataa ataacgacga tcaagattat caaagccatg atggtcaaca
     1921 tctagcatat tcagtgcagc acacgccact tcacctgtaa ccacgccatt actttttact
     1981 tcggcataat cacgcacacg acgtaataaa cggttggcga tacgtggtgt gccacgcgaa
     2041 cgacgcgcaa tttcaaccgc gcctgcttct tccataggga catttaataa tcgtgctgaa
     2101 cggctgacaa tggtgcttaa atcgacaaca ttataaaact ctaaacgctg gacaatgcca
     2161 aaacgatcac gtaatggcga ggttaacaaa cctgcacgag tagtggcggc aacaagggta
     2221 aaagggggta agtctagttt gattgagcgt gcaccaggcc cttcaccaat aacaatatcc
     2281 agttggtagt cttccatggc tggatataag atttcttcaa taacaggtga taaacgatga
     2341 atttcatcaa taaataagac atcgccttct tctaagttcg tcagtagggc ggctaaatcg
     2401 cctgcctttt ctaaaacagg gccactggtc gatttgagtt cgccttccat ttcacgggca
     2461 ataatattgg caagggtggt ttttcccaaa ccgggtgggc caaaaataag ggtgtggtct
     2521 aaggcttcgc cgcgtgtttt ggccgcatga ataaaaattt ccatttgttc gcggactttg
     2581 ggttggccaa tatagtccgc caaactcgtt ggtcgaattg cgcggtcgat aatttcttct
     2641 tggcgttggg cgtggggagc aataattctg tcgtgtgcca tgatggtgag ctgtaaggtc
     2701 aattattttg tgggcatggt agcatagctt aattggctaa agataaacaa ttgtggctgc
     2761 atggcagttt ggcgtcataa tttgacgttt ggcataagac tcaaaaagaa aaaaactaat
     2821 aatctttgtg tttagttttc aagctttgtt attggtgttg gctgttaatt ctactcagaa
     2881 aatcaaaaca ctctagtcct atacaatcgt cacgtaaagt acaaaattta tcgccttttc
     2941 catttgtaat gcactgacac acaatgacgt tttcatatga ttagccattg ccca
//
LOCUS       DAOQMB010000277         2991 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18618_length_2991_cov_15.655654, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000277 DAOQMB010000000
VERSION     DAOQMB010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18618_length_2991_cov_15.655654"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLY05_08745"
     CDS             complement(<1..945)
                     /locus_tag="PLY05_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC03427.1"
                     /translation="MNKVGVVLFDGCWGSQLMTILDFMAIYQRLANNKILISSVDYQL
                     YALRQIGVTLANGMRVETQPLTDTITHDFLIIVGIEYRHLQQLLTSQQLQHSVLATVL
                     CQSTRVLTVSTASFLIAELNLWPTQHLVTHWQFEQVFRKRYPHYKLKSEVEFVDNGQL
                     CSAMGINGALYCLSQYLLRFGEKNLVERCLTIMSKNLDSAEHLWLSDSFAYKQHHYPH
                     ILAIQQFIDHHYQDNLCLAFLANKFHLSESTLKRHFKLATTLSISDYYQLIRIARMKY
                     LLVNTTLTIEQICQEIGYADSRFVRRLFVKMTGQSAKAF"
     gene            1047..1844
                     /locus_tag="PLY05_08750"
     CDS             1047..1844
                     /locus_tag="PLY05_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003152688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03428.1"
                     /translation="MSSAITCPHQDIYINHPQGQIFSRIWTPLNYQASLAPIILLHDS
                     LGCVAVWRDFPMQLAICSGRQVIAYDRLGFGQSSARLKKIAFSFIEDEAKNDFALVKQ
                     QLNIKEFVVLGHSVGGSMAVHCAASYPQHCQGLITIAAQAFTETNTRVGLQQAKIQFA
                     DPQQFARLIKYHGQKSAWVLSSWLDTWLDAAFDHWSLAEVLPKVLCPTLAIHGQFDEY
                     GSMAHPKTIAQSVSGQGRWWIVEGAYHVPHREKNEEVLVALKEFLTP"
     gene            2001..2657
                     /locus_tag="PLY05_08755"
     CDS             2001..2657
                     /locus_tag="PLY05_08755"
                     /inference="COORDINATES: protein motif:HMM:NF036520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC03429.1"
                     /translation="MARSDTMQATQRPRQRVSPVQERSRQKLNTILDATANLLVQQGV
                     EATTMLAIAEAADLSPATVYHYFENRLAIFAALAERTMTMVDNNLSERLNAFAVSNEQ
                     STHELLQTLYQLYHQTPGYVSLLTALRAEPSLQALVRESNRRIADVICEVLVRRTSLA
                     LRRAQRLGWILSQSCDQVLQEALLSTPEEAQALLEELTEMVDTLLVHYIQNSKNSALN
                     "
     gene            complement(2650..>2991)
                     /locus_tag="PLY05_08760"
     CDS             complement(2650..>2991)
                     /locus_tag="PLY05_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC03430.1"
                     /translation="PYQAAYAGTKAFVTNFAQSLALEQHQQNLSLTVFSPGGIDTEMT
                     SNSRLKYFQNTPLLQSVASCASEGIAAMQARKNLAVSGLLNRQQLFVSRFLPRQLIGL
                     ITRKAYAQAFN"
BASE COUNT          873 a          603 c          638 g          877 t
ORIGIN      
        1 aaaagccttg gccgattgcc ctgtcatttt gacaaataaa cgtctgacaa aacgggagtc
       61 ggcatagcca atttcttgac aaatttgctc aatggttaag gtggtattaa ctaataagta
      121 tttcatgcgg gcaattctaa ttaattgata ataatcagaa atactcaagg tcgtggctaa
      181 tttaaagtga cgttttaagg tgctttcact cagatgaaat ttattggcta aaaaagctaa
      241 acaaagatta tcttggtagt gatggtcaat aaactgttga atcgccaata tatgtggata
      301 atgatgttgt ttgtaggcaa aactatcact caaccataaa tgctcagcac tatcaagatt
      361 tttactcata atcgttaagc aacgttcaac aagattcttt tcgccaaaac gcaataaata
      421 ttggctaaga caatacaacg ccccattaat gcccattgca ctacataact gaccgttgtc
      481 tacaaactca acctcacttt tgagcttgta atggggataa cgtttgcgaa aaacctgttc
      541 aaattgccaa tgagtgacga ggtgttgcgt tggccataga tttaattctg caatcaaaaa
      601 actggccgtt gataccgtta acactcgtgt gctttgacat agcacagtgg ccaaaacgct
      661 gtgttgtagt tgttggctag ttaataattg ttgcaaatgg cgatattcaa tacccacgat
      721 aatgagaaaa tcatgtgtta tcgtatctgt taagggctga gtttctacgc gcataccatt
      781 ggctaaagtg actccaattt gtctaagagc ataaagctga taatcaacag agctaatcaa
      841 tattttgttg ttagcaagac gttgataaat agccataaaa tctaaaattg tcatcagttg
      901 agaaccccaa caaccatcaa ataacaccac acctacttta ttcattgaac taatccgccc
      961 gaaacttaat ctattacacc atcgtttaat tatccgcttt tttttaagat aaatttcatc
     1021 ataaaacaag ttgttgagtc aaaaatatgt cgagtgctat tacttgtccc catcaagata
     1081 tttatatcaa tcacccacaa gggcaaattt ttagccgtat ttggacacct cttaattatc
     1141 aagcaagttt agcccccatt atattgttgc atgactcttt aggttgtgtg gcggtgtggc
     1201 gtgactttcc catgcagttg gcgatttgtt caggccgaca agtgattgct tatgatcgtt
     1261 taggttttgg tcaatcgtca gcacgtctta aaaaaattgc ttttagtttt attgaagatg
     1321 aagccaaaaa cgattttgcg ttggttaaac agcaacttaa tatcaaagaa tttgtggttt
     1381 tggggcatag tgtggggggg agtatggcag tgcattgtgc ggcgtcttat ccacaacatt
     1441 gccaaggctt gattaccatt gctgcccaag cctttaccga aaccaatacc cgtgttggtt
     1501 tgcaacaagc aaaaatacaa tttgctgatc cacaacagtt tgctcgttta atcaagtatc
     1561 atggccaaaa atcggcgtgg gtgttgtcat catggttgga tacttggtta gatgcggctt
     1621 ttgatcattg gtctttagca gaggtgttgc ccaaggtgct ttgtccaaca ttggcgattc
     1681 atggtcagtt tgatgagtat ggcagcatgg cacatccaaa aactattgca cagtcggtga
     1741 gtggtcaagg tcgatggtgg atagttgaag gggcatatca tgtgccgcat cgtgaaaaaa
     1801 acgaggaagt gttagtggcg ttaaaagagt ttttaactcc atgaaatgag ttgagagatt
     1861 gtttgaaaaa taacgagatt gaaaaactca atccatttgg gcaatgctga taaatttgct
     1921 aaaatcgcca tggttatttt aggacttacg cggttgatga aaggcctaag tcctgtattt
     1981 tttattcaaa ggataagctc atggcacgtt cagacacaat gcaagccact caacgtccac
     2041 gccagcgtgt gtcacccgta caagaacgtt cacggcaaaa gctcaatacc attttagatg
     2101 ccaccgccaa tttgttggtg caacaaggtg ttgaagccac caccatgctc gccattgcag
     2161 aagccgccga cttatcgcca gcaaccgtgt atcactattt tgaaaatcgt ttggcgattt
     2221 ttgccgcctt agccgaacgt accatgacca tggttgataa taatttgtca gagcgtttaa
     2281 acgcctttgc ggtatctaac gaacaaagca cccacgaatt actccaaacc ttgtaccagt
     2341 tgtatcatca aaccccaggt tatgtctcgt tgttgaccgc gttacgtgcc gaaccttcct
     2401 tgcaggcctt agtgcgtgaa tcaaatcgcc gtattgccga tgtgatttgt gaagtattgg
     2461 tgcgacgtac ctccttggcg ttacgtcgcg cccaacgttt aggctggatt ttaagccagt
     2521 cgtgtgatca agtattgcaa gaagcgttat tatccacgcc cgaagaagcg caagccctat
     2581 tagaagaact caccgagatg gtggatacac tgttagtcca ctatattcaa aactctaaaa
     2641 attctgcgct taattaaacg cttgggcata cgctttgcgt gtaatcaaac caattaattg
     2701 ccgtggcaaa aaccgactca caaaaagctg ttggcgattt agcaaaccag aaacagcgag
     2761 atttttgcgt gcttgcatgg cggcaatgcc ttcactggcg catgatgcta cgctttgcag
     2821 taatggcgta ttttgaaaat actttaaacg actattgctg gtcatttcgg tatcaatgcc
     2881 acctggtgaa aagaccgtta aagacaggtt ttgctgatgc tgttcgaggg caaggctttg
     2941 ggcaaaattg gtgacaaaag cttttgtacc tgcataggcc gcttggtagg g
//
LOCUS       DAOQMB010000278         2988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18647_length_2988_cov_13.479373, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000278 DAOQMB010000000
VERSION     DAOQMB010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18647_length_2988_cov_13.479373"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..602)
                     /locus_tag="PLY05_08765"
     CDS             complement(3..602)
                     /locus_tag="PLY05_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNC03431.1"
                     /translation="MSKLILDPTTIQKWRQDIVFKENVLGDDYTFHSTWGIFSPEAVD
                     EGSRLLLEHVDFKATDNCLDVGCGYGVLGMVCAKKAPQGTSLLLDKDFVAVDYARKNC
                     ALNGLNNTEVILSNGFSAVGDRKFDIILSNLPAKVSKEQHYLYLYDALQHLSEGGRFY
                     VVTINGLREFISRTFKEVFGNYEKLKQGKTYTIAMAEKR"
     gene            complement(599..952)
                     /locus_tag="PLY05_08770"
     CDS             complement(599..952)
                     /locus_tag="PLY05_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03432.1"
                     /translation="MKSLNFLFVATIITSLTACSSAPEKGSFAERLNLRGDQAKGIAK
                     QWDEGQSLVTKGEKMKVTGQKTIEDGKSKITKGERMIVDGDALINKGKQMMTDSESAY
                     QNMRATPIPLPATQP"
     gene            complement(1034..1801)
                     /locus_tag="PLY05_08775"
     CDS             complement(1034..1801)
                     /locus_tag="PLY05_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNC03433.1"
                     /translation="MRIVSVNVNGLRSAERKGFFDWLENSQADVVCLQEIRIQSHQME
                     LAHQPAGWFHHFFPAQKAGYAGTAIYSRQQPDLVTQGLGFSLCDDEGRYLAAQFGDLT
                     IASLYLPSGSSSPEAQAKKDDFLQQFLPLLSQWRSEQKSMIICGDWNIAHKEIDLKNW
                     KGNLKNSGFLPHERAWLDTLYQELGYVDAYRQLYPTEEAQAYTWWSNRGQAWQNNVGW
                     RIDYQVTSPDFAHKVKQAFVYKDSRFSDHAPLIIDYQ"
     assembly_gap    1820..1919
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2075..>2988
                     /gene="gshB"
                     /locus_tag="PLY05_08780"
     CDS             2075..>2988
                     /gene="gshB"
                     /locus_tag="PLY05_08780"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HNC03434.1"
                     /translation="MKLLIVMDDIRHINIKKDSSIAMLWAAAKRGYELFYAEQDDLFI
                     ENGEAYGQLKPLQVFESKEKWHEFGEAQNIALAEMDVILMRKDPPFDMRFIYTTYILE
                     RAEQAGVLVVNKPQSLRDCNEKLFATQFAHCMVPTLVSSQMPRIKQFIAQHQDVIVKP
                     LDGMGGTGIFRLQHQGLNIGATLETLTQNGTLPVMAQRYIPEIVKGDKRILVIDGKPV
                     PYCLARVPQQGETRGNLAAGGSGVPQLLTPRDYWLASQVGQELKKRGLLFVGLDVIGD
                     YITEINVTSPTCIREIDARYQFGIADRL"
BASE COUNT          872 a          570 c          566 g          880 t
ORIGIN      
        1 gattaacgct tttcagccat agcaatcgta taggttttac cctgttttag cttctcataa
       61 ttaccaaata cttccttaaa agtacgcgaa ataaactcac gtaaaccatt aatcgtcact
      121 acataaaagc gtccaccttc gcttaaatgc tgtaaggcat catataaata caaataatgt
      181 tgctctttgc tcactttagc aggtaagtta gacaaaatta tatcaaactt acgatcacct
      241 acggcactaa agccattact taaaatcact tccgtattgt ttaagccgtt cagtgcgcaa
      301 tttttacgag cataatcaac agcaacaaaa tctttatcta ataataaact tgtaccttgt
      361 ggtgcttttt tggcacaaac catacctaaa acaccataac cacagccgac atctaaacag
      421 ttatcagttg ctttaaaatc aacatgttcg agtaacagac gtgaaccttc gtcaacagct
      481 tcgggagaaa aaatgcccca agtggaatga aaggtatagt catcacctaa gacgttttct
      541 ttaaaaacaa tatcttgtcg ccatttttgg atggtagttg gatctaaaat taatttgctc
      601 atggttgtgt cgctggcaga ggaataggag tggcacgcat attttgatac gcactttcgg
      661 agtcagtcat catttgtttg cctttgttaa ttaaggcatc accatcaaca atcattcgct
      721 cacctttggt gattttgctt ttgccatctt caatggtttt ttggccagtc actttcattt
      781 tttcgccttt agtcactaaa ctttggcctt catcccattg tttagcaata cctttggctt
      841 ggtcaccacg taaatttaaa cgttccgcaa aactaccttt ttcgggcgca ctagaacaag
      901 cggttaaact agtaataatt gtagcaacaa ataaaaaatt taaagatttc atgatggata
      961 ctccatttta ttgtttaatc aaatagtcat taaacaatat cgctaaaact aacgattgat
     1021 aagaagattt ttgttattgg taatcaataa ttaatggtgc atggtcagaa aagcgactgt
     1081 ctttataaac aaaagcttgc ttaactttat gagcaaagtc tggactagtc acttgataat
     1141 caatacgcca accgacattg ttttgccatg cttgaccacg atttgaccac catgtatagg
     1201 cttgcgcttc ttcggttggg taaagctgac gataagcatc cacatagcct aattcttgat
     1261 acaacgtatc taaccaagcg cgttcatggg gtaaaaagcc agagttcttt agattgcctt
     1321 tccagttttt taaatcaatt tctttgtggg caatattcca atcaccacaa ataatcatcg
     1381 acttttgctc actgcgccat tggcttagaa ggggtaaaaa ctgttgcaga aaatcatctt
     1441 ttttggcttg agcttcgggt gagctagagc cagacggcaa atataaggag gcaatagtta
     1501 aatcgccaaa ttgtgcggct aaataacgac cttcatcgtc acataaacta aaacctaaac
     1561 cttgagtaac aagatcaggc tgttgacgag aataaatggc tgtacctgca taacccgctt
     1621 tttgagcagg aaagaaatga tgaaaccagc ccgcaggttg atgcgctaat tccatttgat
     1681 ggctttgaat tcgaatttct tgcaaacaca ccacatcggc ttggctgttt tctaaccaat
     1741 caaaaaagcc tttacgttcg gctgagcgta aaccattaac gtttactgag acaattctca
     1801 ttgtttttgc cttatcttan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1921 atattcctgt cgtgtctatc attaaattag atcatattgt tgaatttttg cgtgagcgtg
     1981 gtggtcatca agaattggtc aatagtatga gacaatatcg tgctaaatat ggtgcaaaag
     2041 atttaagcga gtggggcgaa gaagtatgaa gcccatcaaa ctattgatag tgatggatga
     2101 tattcgtcac atcaatatca aaaaagattc cagtattgcc atgctttggg ctgctgcaaa
     2161 acgtggctat gagttgtttt acgccgagca agatgatttg tttattgaaa atggcgaagc
     2221 atatggtcaa ttaaaaccct tgcaggtatt tgaaagtaaa gaaaaatggc acgaatttgg
     2281 cgaagcgcaa aatatcgctt tggccgaaat ggatgttatt ttgatgcgca aagatccgcc
     2341 ttttgatatg cgctttatct acacgacata tattttagag cgtgccgaac aagctggcgt
     2401 tttagtggtc aataaacctc aatctttacg tgattgtaat gagaaattgt ttgctacaca
     2461 gttcgctcat tgtatggtgc caaccttagt atccagccaa atgccgcgca ttaaacaatt
     2521 tattgcgcaa catcaagacg tgattgttaa accattggat ggcatgggtg gaacaggtat
     2581 ttttcgccta cagcatcaag gattaaatat tggtgcaacc ctagaaacac tcacccaaaa
     2641 tggcactttg cctgtgatgg cacaacgcta tattcctgag attgtcaaag gggataagcg
     2701 aattttggtc attgatggca aaccagttcc ctattgtttg gcgcgtgtgc cacaacaagg
     2761 tgaaacgcgc ggtaatttgg cggcaggtgg cagcggtgtg ccgcaattat taaccccacg
     2821 agattattgg ttggctagtc aagttggcca agagctaaaa aaacgtggtt tgttatttgt
     2881 tggtttagat gttattggtg actatatcac cgagattaat gtcactagcc caacctgtat
     2941 tcgggaaatt gatgcacgtt atcagtttgg gattgccgac cgactaat
//
LOCUS       DAOQMB010000279         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_18679_length_2985_cov_16.337543, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000279 DAOQMB010000000
VERSION     DAOQMB010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18679_length_2985_cov_16.337543"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..345
                     /locus_tag="PLY05_08785"
     CDS             40..345
                     /locus_tag="PLY05_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03435.1"
                     /translation="MLEQDLRALNSRIDSLINKVQTLSAENNSLKQALGEFQSVKTKI
                     ESLTTENDRLRQQENVWLTERQKLLAEREELSRKNDHARARVEAIILRLKALETPEA"
     gene            415..708
                     /locus_tag="PLY05_08790"
     CDS             415..708
                     /locus_tag="PLY05_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNC03436.1"
                     /translation="MSSQNVDLVIFDKTYKIESPTEEQESLLAAAKYLDGKMKEIRQA
                     ARGLESERVAVLAALNICHELFQSKQQADMAQKIGQALQILTGKIDKTLAQNS"
     gene            778..975
                     /gene="ssrS"
                     /locus_tag="PLY05_08795"
     ncRNA           778..975
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLY05_08795"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            988..1569
                     /locus_tag="PLY05_08800"
     CDS             988..1569
                     /locus_tag="PLY05_08800"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HNC03437.1"
                     /translation="MSNQRQLLRKTLRQKRKSLSFYQQKKSAHQLANQFRHLTIFKTA
                     NKIAVYQATDGEINPYCLMNLIWAYGKQCYLPVLRRFPNQELGFVRVYKTSRLHRHRY
                     GFKEPKAKHRLFIYQLDLVCLPLVGFDATGKRLGMGGGFYDRSLARSPKKHVYKLGLA
                     HDCQQIEQLQSQSWDIPLDAIITPSQLIRVTNC"
     gene            complement(1566..1784)
                     /locus_tag="PLY05_08805"
     CDS             complement(1566..1784)
                     /locus_tag="PLY05_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03438.1"
                     /translation="MPTERNSPLEDDFTIHTQFYVAVRRATGRVIDLVWFKQNKEYAR
                     AVLDYADAISDREVRDLSRRLRFAKRLH"
     gene            complement(1890..2543)
                     /locus_tag="PLY05_08810"
     CDS             complement(1890..2543)
                     /locus_tag="PLY05_08810"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005278751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNC03439.1"
                     /translation="MNLAIFDLDNTLIAGDSDHAWGEFLVNKNLVDSQWYKAQNDKFY
                     ADYCAGQLNIIAYSEFVFSFLAQHDTAYLQQLHQQFMSEVIRPIMLPKAQALIAEHCA
                     AGDELLIITATNRFITQAIGEAFGIPQVLATEPEKIDGRYTGKVLGVPCYKEGKITRY
                     QAWLAQQNKRFDKVTFYSDSHNDLPLLSIVDVPVVVDADAKLLAHAQEKGWQILSLR"
     gene            complement(2548..>2985)
                     /locus_tag="PLY05_08815"
     CDS             complement(2548..>2985)
                     /locus_tag="PLY05_08815"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004968267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNC03440.1"
                     /translation="VWQQQQIIAQFIISSALKGAGEIQGSEQTPRGQHIIRAKIGQNQ
                     PINSVFKARRPTGEIYNAQLAAQFPTRDWILTRIMWLSGCELGKNRGGRYDTMRRYIY
                     IHGTPDTEPMGIPKSHGCIRMRNEDIIQLFDLVPVYTPVIIHE"
BASE COUNT          946 a          607 c          552 g          880 t
ORIGIN      
        1 tctatattag ccgccaaatg cttgtagtgc gacaaaaaaa tgctagaaca agacttacgc
       61 gcgttaaatt cgcgcattga cagcttaatt aataaggttc aaaccctcag tgccgaaaat
      121 aatagcttaa aacaagcctt aggtgagttt caaagcgtta aaacgaaaat tgagagttta
      181 accaccgaaa acgacagact tcgtcaacaa gaaaatgttt ggctgactga acgccaaaaa
      241 ctgcttgctg aacgtgaaga attatctcgc aaaaacgacc atgcgcgtgc gcgtgtcgaa
      301 gcgattattt tacgtttaaa agccttagaa acgcccgaag cttaatttat tgacaacatt
      361 atgcactaat ttgctgaatt agttgtgttg tctgtgcttg ttgaggaaag aaaaatgtct
      421 agtcaaaacg ttgatctcgt tatttttgat aaaacttata aaattgaatc acctaccgaa
      481 gaacaagagt ctttattggc tgctgctaaa tatttagacg gcaaaatgaa agaaattcgt
      541 caagcagcgc gtggtttaga aagtgaacgt gttgcggttt tagcggcctt aaatatttgc
      601 catgagttat ttcaaagtaa gcagcaagcg gatatggctc aaaaaattgg ccaagctttg
      661 cagattttga caggcaaaat tgataaaact ttagcccaaa atagctaatt tttgttttaa
      721 ctgcttaact actgagcatg aatacttgca agttatgttc agtcggttat actgaacaca
      781 tctctaaagt gtacgccaat ctatcgcgtc ccctgagccg ataaattttt cttatggaaa
      841 tgacgtattg ttttggtgtg caggtctgct aagccgcaag gcatttagtc cagaaagcct
      901 aaaaaagcat acttgtttcc tccttgaacc tctgggttca agggtctaga tggatagcgg
      961 cactttggag ctttatctaa actttttatg tccaatcaac gtcaactact gcgtaaaacc
     1021 ttacgtcaaa aacgtaaaag tctgtcgttc taccaacaaa aaaaatcggc acatcaactt
     1081 gctaatcaat ttcgtcatct cactatcttt aaaaccgcca ataaaatagc agtttatcaa
     1141 gcgactgatg gtgaaataaa tccttattgt ttaatgaatc taatatgggc ttatggtaaa
     1201 caatgctact tacctgtact cagacgattt cccaatcaag aattaggttt tgtgcgtgtt
     1261 tataaaacga gtcgtctgca ccgccaccgc tatggtttta aagagccaaa ggctaagcat
     1321 agattattta tttatcaact agacttggtt tgtctgcctt tggttggatt tgatgccaca
     1381 ggtaagcgat taggcatggg tggcggattt tacgatcgaa gcttagctcg ctccccaaaa
     1441 aaacatgttt ataaattagg tttagctcat gattgccaac aaatagaaca attacaaagc
     1501 cagtcttggg atattccttt agatgctatt atcacacctt cccaattgat tcgggtaact
     1561 aattgctaat gtaaacgctt agcaaagcgt aaacgtctag ataaatctct gacttcgcga
     1621 tcgctgattg catcagcata atccaataca gcacgagcat attctttatt ttgtttaaac
     1681 caaacgagat caataacacg ccctgtcgca cgccgtaccg caacataaaa ttgagtatga
     1741 atggtaaagt catcttctaa tggcgagttt cgttcagtag gcataatagt ctcgtccaaa
     1801 aactaagcac tctgaacaat aacatccata agacatacaa tcaggaaata ttgttcaaca
     1861 attgaacaac tccatcactt atgccaagat caacgcaatg acaaaatctg ccaacctttc
     1921 tcttgggcat gggctaataa tttagcatca gcatccacca caacaggcac atcaacgatt
     1981 gataacaaag gtaaatcgtt gtgtgaatcc gaataaaaag ttactttatc aaaacgttta
     2041 ttttgctgtg ctaaccaagc ttgataacgg gtaattttac cttctttata acaaggcaca
     2101 cccaacactt tacctgtata acgcccatct attttttctg gctcagtggc taatacttgt
     2161 ggaataccaa aagcctcacc aattgcttga gtaataaaac gattagttgc agtaataatc
     2221 aacaattcat cccctgcggc acaatgctct gcaattaaag cttgtgcttt aggcaacatg
     2281 attggacgaa tcacttcact cataaattgt tgatgtaatt gttgtaaata cgcagtgtca
     2341 tgttgtgcta aaaagctaaa gacaaattca ctataggcga taatatttaa ttgacctgca
     2401 caataatcgg cataaaactt atcattttgg gctttatacc attggctatc gactaaattt
     2461 ttattaacca aaaactcgcc ccatgcgtgg tcagaatctc ctgcaattaa ggtattgtct
     2521 aaatcaaaaa tggctaaatt catggtctta ctcgtgaatg ataacaggcg tataaacagg
     2581 gactaaatca aaaagttgga taatatcttc attgcgcatt ctaatacagc catgtgattt
     2641 gggaatgccc atcggctcgg tatctggagt gccatgaata taaatataac ggcgcatggt
     2701 atcataccga cctcctcggt ttttacccaa ctcacatccc gataaccaca taatacgcgt
     2761 taaaatccaa tcccgtgtgg gaaactgtgc ggctaattgt gcattataaa tttcacccgt
     2821 tggtcgccgt gctttaaata cgctgttgat gggttgattc tggccaatct ttgcacgaat
     2881 aatatgttgt ccacgtgggg tttgctcact gccttgtatt tcacctgccc cctttaaggc
     2941 ggatgaaata ataaattgtg cgattatttg ttgctgctgc catac
//
LOCUS       DAOQMB010000280         2954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19027_length_2954_cov_14.432218, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000280 DAOQMB010000000
VERSION     DAOQMB010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19027_length_2954_cov_14.432218"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>1234
                     /locus_tag="PLY05_08820"
     CDS             21..>1234
                     /locus_tag="PLY05_08820"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020752838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HNC03441.1"
                     /translation="MLNWDDFYAEEKPKAKAPATPAAPAQTVSQKPTTPSPAMAPAQP
                     ITTTAALDRANAALANLDVAAGLEDLEMGAARIQVDDKRMINCKADLNQLVPFKYDWA
                     WQKYLDGCANHWMPQEINMTKDIALWKTPEGLTDDERLIVMRSLGYFSTADSLVANNL
                     VLAVYRLITNPECRQYLLRQAFEEAIHTHAYQYCIQSLGMDEGEVFNMYREVPSVAKK
                     AAWSISHTHALSNPNFKTGTAEADQELLRNLIGFYGVTEGIFFYCGFTQILSMGRRNK
                     MTGVAEQFQYILRDESMHLNFGIDVINQIKIENPHLWTAAFQQEVIQMILEGTQLEIE
                     YARDTMPRGVLGMNAAMMEEYLQFIANRRLTQLGLPEQFAGVKNPFPWMSEIMDLRKE
                     KNFFETRVTEYQV"
     assembly_gap    1235..1334
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1335..1912)
                     /locus_tag="PLY05_08825"
     CDS             complement(<1335..1912)
                     /locus_tag="PLY05_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03442.1"
                     /translation="MIWFLLIVMFIVIVSYLNWQQKIKRMDVIKRLVFDASIQRRVAE
                     TYPHLTPTQVKEVLEGLRDYFYCVLMMRGKGMLSMPSQAVDEAWHAFILSTKAYESFC
                     QRAFGKFLHHHPAETMQTRTQATIGIKRTWNLACRHEQINPALPSRLPRLFALDAMLM
                     IPSGFVYSLNCMAANNPSDSSYCASDIGGGCGG"
     gene            2104..>2954
                     /locus_tag="PLY05_08830"
     CDS             2104..>2954
                     /locus_tag="PLY05_08830"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNC03443.1"
                     /translation="MSDNNKHATTPTKRFMRLAGMTASITGKVMSNSVKSLVGSPEQK
                     AEARSEMYHEIGKQIANTLGEMKGAVMKVGQIASQYQDIFPPEIAAAMTKLQKEAPPM
                     PFSVIEQQILHELGAKPHEKFAEFDETPFAAASIGQVHKAKLPTGEDVIVKVQYPGVE
                     ECCESDLKHLRMALKLAGVIKLSKEVQEKLFDEIRTSLHAELDYLQEAANLREFKAFH
                     ANDDKLIIPSVFDDFSSKRVLTLSYEAGDSLNTAKHYPHHVRNEIGERLFHTLCEELY
                     HLQALHC"
BASE COUNT          882 a          591 c          591 g          790 t
ORIGIN      
        1 tattttaaga gagaaccacc atgttaaatt gggatgattt ttatgccgaa gaaaagccta
       61 aggctaaagc acctgcaacg ccagcagctc ctgcacaaac agtttcgcag aagccaacta
      121 ctccgtcgcc agcaatggca ccagctcagc ccatcacaac cacagctgcc cttgaccgag
      181 caaacgcagc tcttgccaat ttagatgtgg cggctggcct agaagattta gaaatgggcg
      241 cagcgcgtat ccaagttgac gataagcgca tgattaattg taaggccgac cttaaccaac
      301 tggtgccgtt taagtacgac tgggcatggc aaaaatattt agatggttgt gcaaaccact
      361 ggatgccaca agaaattaac atgaccaaag acattgcctt gtggaaaaca ccagaaggtt
      421 taaccgatga cgagcgttta atcgtcatgc gttctttagg ttatttttca acggctgata
      481 gcttagtggc gaataacttg gttttagcgg tttaccgttt aattaccaat ccagaatgcc
      541 gccaatattt attgcgtcaa gcctttgaag aagcgattca cactcacgcc taccaatact
      601 gtattcaatc attgggcatg gatgaaggcg aagtgtttaa tatgtatcgt gaagtgccat
      661 ctgtggccaa aaaagccgca tggagcatta gccatactca tgctttatct aatcccaact
      721 ttaaaacagg cacagccgaa gccgaccaag aattgttacg caatttaatt ggtttttatg
      781 gcgtgaccga aggcattttc ttctattgtg gttttaccca aattttaagt atgggtcgcc
      841 gcaacaaaat gactggcgtt gctgagcaat tccaatacat tttgcgtgat gaatcgatgc
      901 acttaaactt tggtattgat gttatcaacc aaatcaaaat tgaaaatcca catttgtgga
      961 cggctgcttt ccaacaagaa gtcattcaaa tgattttaga aggcactcaa ttagaaattg
     1021 aatatgcgcg tgacactatg cctcgtggcg tattgggcat gaatgcggcc atgatggaag
     1081 aatacttgca gtttattgct aaccgtcgtt taacccaatt aggtttacct gagcagtttg
     1141 ctggcgttaa aaatccattc ccgtggatga gtgagattat ggatttacgc aaagagaaaa
     1201 acttctttga aacgcgtgtg actgagtatc aggtnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnccccca cagccgccac caatgtcact agcacaataa ctgctatcac
     1381 ttgggttatt agcagccata caatttaaac tataaacaaa cccacttgga atcattaaca
     1441 tcgcatctaa agcaaataat ctaggtaatc gtgatggcaa agcaggatta atttgctcat
     1501 ggcgacacgc caaattccat gttcgtttaa tgccaatcgt ggcttgagtt cgtgtttgca
     1561 ttgtttcggc aggatgatga tgtaaaaact taccaaaggc gcgctggcag aaactttcat
     1621 aggctttggt tgataagata aaggcatgcc atgcttcatc aactgcttgt gaaggcatac
     1681 tcaacatacc tttaccacgc atcattaaca cacaataaaa ataatctctt aagccttcta
     1741 agacttcttt cacttgtgta ggcgttaaat ggggataagt ttcggcaact cgacgttgaa
     1801 tagaggcatc aaacactaaa cgtttaatca catccatgcg ttttattttt tgttgccaat
     1861 tcaaataact gacaatcaca ataaacatga caattaaaag aaaccagatc atttttattc
     1921 tccttcaaga ctcacgcttt tagccttgtt tttttgttat ttaagttgct attagttata
     1981 tgactaaaat atgacaaaat gatgacaaaa atatcagcgc cttgatgaca aatgacagat
     2041 tgacaagctc caagataact ttaggataag cttttacccc ttatatgttt aggatgcgtt
     2101 agcatgagtg ataataataa acatgccaca acccccacca aacgttttat gcgtttagct
     2161 ggcatgacag caagtattac agggaaagtc atgagtaatt cggtcaaaag ccttgttggc
     2221 tcacccgaac aaaaagccga agcccgtagt gaaatgtatc atgaaattgg caaacaaatt
     2281 gccaataccc ttggggagat gaaaggtgcg gtcatgaagg tcgggcaaat tgcctcacaa
     2341 tatcaagaca tctttccgcc cgaaattgct gctgcaatga ctaaattgca aaaagaagca
     2401 ccgcccatgc cttttagcgt gattgaacaa caaattttgc atgagctggg cgcaaaacca
     2461 cacgaaaaat ttgccgaatt tgatgaaaca ccgtttgctg cggcgtctat tggtcaagta
     2521 cacaaagcca aattgccgac aggcgaagat gtcatcgtta aagtgcaata tccgggtgtt
     2581 gaagagtgtt gtgagtctga tttaaaacac ctacgcatgg cgctcaaatt agcaggtgtg
     2641 attaagctga gcaaagaggt acaagaaaaa ctctttgatg aaattagaac ctctcttcat
     2701 gccgaattag actatttgca agaggccgct aatttacgtg aatttaaagc cttccatgcc
     2761 aacgatgata agctgattat tccttctgtt tttgatgatt tttcgagtaa gcgggtgtta
     2821 acgttaagtt atgaagcggg cgatagttta aataccgcga aacattatcc gcatcatgtg
     2881 cgtaatgaaa ttggtgaacg cttatttcat accttgtgtg aggagctcta tcatctgcaa
     2941 gccttacatt gtga
//
LOCUS       DAOQMB010000281         2950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19077_length_2950_cov_15.821762, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000281 DAOQMB010000000
VERSION     DAOQMB010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19077_length_2950_cov_15.821762"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2950
                     /locus_tag="PLY05_08835"
     CDS             <1..>2950
                     /locus_tag="PLY05_08835"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HNC03444.1"
                     /translation="IANFARSQAVFSQDIQIATTLVISFPSSEADSIRINNFFNVDAT
                     GKIETYSKDAIETLIFKDGTVLTAAQIVGIFNNTPPQIINDPLNAEVNEDGSITLDLL
                     SNVSDVNGQSLTITKVDLDDPTQGSLGFLDSQEIVFTPAANFNGAVSLTYTVSDGVAS
                     VTGKTIIQVMPINDAPTALLPLDEQSVDEGQSFVFTIPTEAFTDIDGDNLAYSASLVD
                     GSSLPSWLSFNDKTQTFSGIPSFSDAGTLQVKVVATDPDGLFTSQIFTLDINDVNQAP
                     IAISDAPLLITNEDTDLAIDLSLRIADPDGDDFTIESASSTAGTVVINADKTLTYIPT
                     ADFYGIADLTYTVKDSQGATFTKTESITVNAVNDAPVAASPSPITNQQGNEGEAFNYK
                     IPVNAAFNDVDSDEGGIIYSVAGLPDWLSFDSRKFILSGTPTFNDAGKHIITVTATDD
                     GGLSASTSFELTINNVNREPVVSINPIYIDEDSPIQDIDVVSSIYDPDTNDTISISDV
                     SVDASQGVAILNADKTISFTPANDFNGTAIINFVVSDGTAFVNSSVEVVVQPVNDAPV
                     LTSTPIDLNDPIEDTSYVLYANDLLQGYTDIDGDILSIIKLTVSNGTLVDNNDGSYVY
                     TPNLDYFGNIQLNYYVSDGIAETPVTRTLNVINQNDAPTGTATAILNNGQEDVAYIVK
                     ASDLLQGFSDVDGEALSVINLSANQGTVIDNLDGTFTIQAPQNYNGAINLNYQVTDST
                     YTLSANQTFVLVPVNDAPNTQDIIGNLQINEGQSFLYALSANSFKDLDGDVLTYTATL
                     ENGDPLPNWLIFNQNTLGFSGTPSFSDAGVLQITVVASDGSLTAAQTFALQIQDVNRA
                     PMALDVLGNFSTNEDQSLDNINVLSLVADPDGDPLTVTSAVITAGQGTISINANQTLN
                     FNPTPNFNGSVTISYRVEDGRGGFTDRQDTIVVNPINDAPIAPSTPVVLANGTEDNTY
                     TINVSDLLQGY"
BASE COUNT          905 a          507 c          588 g          950 t
ORIGIN      
        1 attgcaaact ttgcgcgtag tcaggcagta tttagccaag atattcaaat tgcaacaacc
       61 ttagttatta gttttccctc ctcagaagca gacagtattc gcattaataa cttttttaat
      121 gttgatgcca caggcaaaat tgaaacctat agtaaagatg ccattgaaac attgatattt
      181 aaagatggta cagttttaac agcagcacaa attgttggta tttttaataa cacacctccc
      241 caaattatta atgatccatt aaatgccgaa gtcaatgaag atggatcaat taccttagat
      301 ttattatcga atgtttctga tgtcaatggt caatctctaa ccattactaa agttgatttg
      361 gatgatccta cacaaggctc tttgggcttt ttggatagcc aagaaatagt ttttactcct
      421 gctgccaatt ttaatggtgc tgtgagtttg acttataccg tcagtgatgg tgttgccagt
      481 gtaacaggga aaaccattat tcaggtgatg ccaattaatg acgcaccaac cgctttatta
      541 ccattagatg agcaaagtgt tgatgaaggg cagtcatttg ttttcactat tcctaccgaa
      601 gcatttactg atattgatgg cgataattta gcctatagtg cgagtttggt ggatggtagt
      661 tcattgccat cttggctcag ttttaatgat aaaactcaga catttagtgg tattccaagt
      721 tttagtgatg caggtacatt acaagtaaaa gtggttgcaa cagaccctga tggtttattt
      781 acttcacaaa tttttactct agatattaat gatgtgaatc aagcacctat tgccattagt
      841 gatgcaccat tgttaatcac taatgaagat actgacttag caatagatct gtcactccga
      901 attgcagatc ctgatggtga tgactttact attgaaagtg ccagtagtac ggcaggtaca
      961 gtagtcatta atgctgataa aaccttgact tatataccaa cagcagattt ttatggcata
     1021 gcagacctca cttatactgt taaagatagt caaggtgcta cgtttaccaa aacagaatct
     1081 atcaccgtta atgcggttaa tgatgcacca gtagctgctt caccatcacc aatcactaat
     1141 cagcaaggta atgaaggaga ggcatttaac tataagatac ctgtaaatgc tgcttttaat
     1201 gatgtagata gtgatgaagg tggcattatt tatagcgttg ctggtttgcc cgattggtta
     1261 agttttgata gtagaaaatt tattttatcg ggtacaccaa catttaatga cgcaggtaag
     1321 catattatta ccgttacagc aacagatgat ggtggtttgt ctgcaagtac cagctttgaa
     1381 ttaacgatta ataatgttaa tcgtgaacca gttgtgtcta ttaatccgat ttatatagat
     1441 gaagatagtc ctatacaaga tattgatgtt gtatcgagca tttatgatcc tgatacgaac
     1501 gacacaatta gtatttcgga tgtcagtgtt gatgccagtc agggagtggc tattttaaat
     1561 gccgataaga caatcagttt tactcctgca aacgatttta atggtacagc gattattaat
     1621 tttgtggtga gtgatggaac tgcatttgtt aattcttcgg ttgaagtggt tgttcagcct
     1681 gttaatgatg cacctgtatt aacaagtacc ccgattgatt taaatgatcc tatagaagac
     1741 acctcttatg tgttgtacgc taacgattta ttgcagggtt ataccgatat agatggcgat
     1801 atattgagta ttattaaact gacggttagc aatggcacct tagttgataa taatgatggc
     1861 agttatgtgt atacgcctaa tctcgattat tttggcaata ttcaactaaa ttattatgtg
     1921 tcagatggta ttgctgaaac accagttaca cgtacactaa atgtcattaa tcaaaatgat
     1981 gcgccaacag gtaccgcaac agccattcta aacaatggtc aggaagatgt agcgtatatt
     2041 gttaaagcca gtgatttatt acagggcttt agtgatgtgg atggcgaagc attatctgtt
     2101 atcaatttga gtgctaatca aggcacggtc atcgataatt tagatggtac atttaccatt
     2161 caagcaccac aaaattataa tggcgctatc aatttaaact accaagtgac ggatagcaca
     2221 tataccttat cggccaatca aacttttgtc cttgtaccag tgaatgatgc gccaaataca
     2281 caggatatta ttggtaatct acaaattaat gagggacaaa gttttcttta tgctttgtca
     2341 gccaatagtt ttaaagacct agacggagac gtattaacgt acacggctac tttggagaat
     2401 ggtgatcctc taccaaattg gttgattttt aatcagaata cattaggttt tagtggtaca
     2461 ccgagtttta gtgatgcggg tgttttacaa attaccgtgg ttgctagtga tggctctcta
     2521 acagctgcac aaacatttgc attacaaata caagatgtga atcgtgcgcc tatggcctta
     2581 gatgtgttag gtaatttttc aacaaatgaa gatcaatctt tagataatat taatgtttta
     2641 tcattagttg cagaccctga tggtgacccc ttaacagtca cttcggcggt gataactgca
     2701 gggcaaggta caattagtat taatgctaat caaactttaa attttaatcc tacgcctaat
     2761 tttaatggtt cagtgacgat tagttatcgt gttgaggatg gacgaggtgg ctttaccgat
     2821 cgacaagata caattgttgt taacccaatc aatgacgcac cgattgcgcc ctcaacgcct
     2881 gttgtcttag cgaatggcac agaagataac acttatacga ttaacgttag tgacttatta
     2941 caaggctaca
//
LOCUS       DAOQMB010000282         2948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19102_length_2948_cov_17.704459, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000282 DAOQMB010000000
VERSION     DAOQMB010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19102_length_2948_cov_17.704459"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="PLY05_08840"
     CDS             complement(<1..247)
                     /locus_tag="PLY05_08840"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNC03445.1"
                     /translation="MENKSAVQLLAALAQDSRLTIFRLLVQQGETGLPVSKISEALGG
                     MAGATLSFHLKELSHAGLIDAQQQGRFIFYKANLSQMK"
     gene            481..1170
                     /locus_tag="PLY05_08845"
     CDS             481..1170
                     /locus_tag="PLY05_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNC03446.1"
                     /translation="MRHLFFVLLAVFSIAVYADKEDLQSLQKQQNDWYVVKQDNTRHI
                     TTYAKFEDGKKLRSFKIEATVDASLATLASIHFDIANIKKWYWETLESKLINKISDTE
                     YLYYMQFNTPLAPDRDVVIHAKVQPFQDVIHPYLLLQLRAAPSALPVPKNFTRVSAFD
                     MDIKFTPLVDGKTKMEVQGYIDPSGELPAWTVNLFQRQAPYSTMLGLYRMIQKPEYTH
                     NKIPLAFTYMR"
     gene            1357..>2948
                     /locus_tag="PLY05_08850"
     CDS             1357..>2948
                     /locus_tag="PLY05_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020443490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC03447.1"
                     /translation="MLRLTELRLPIDHQPEAIKTAIAKKLAISEADIQHIHIHKRSYD
                     ARKVSQIVLIYIVDIDVNNEAKLLAKFKNDPHVQASPDMAYKFVAQAPNMLTERPIIV
                     GFGPCGILAALVLAQMGFKPIVLERGKEVRQRTKDTWGLWRKNILNPESNVQFGEGGA
                     GTFSDGKLYSQIKDPRHLGRKVLDEFVKAGAPEEILYISKPHIGTFKLVTMVETIRQT
                     IESLGGEIRFQQKVVDVLIDNGQIRGVRLENGEELHSRHVVMALGHSARDTFTMLHER
                     GVYMEAKPFSIGFRIEHPQGMIDRCRFGKFAGHPLLGAADYKIVHHAKNGRAVYSFCM
                     CPGGTVVAATSEPNRVVTNGMSQYSRNERNANSGLVVDIQPSDFGDSSPLAGIAFQRI
                     WESKAFELGGGTYAAPAQLVGDFLAGRPSTKVGTVEPSYKPAVNWCDLSSALPEYVIT
                     AMREALPEFDKKLKGYAMHDAVLTGVETRTSSPLRITRNEHYQSLNVKGLFPAGEGAG
                     YAGGILSAGVDGIKVAEAVALS"
BASE COUNT          844 a          544 c          645 g          915 t
ORIGIN      
        1 ttttcatttg cgacagattg gctttataaa aaataaaacg gccttgttgt tgtgcgtcga
       61 taagacctgc atgactgagt tctttaagat gaaaggataa ggtcgcgcca gccataccgc
      121 ctaatgcctc gctgatttta ctgacaggca aacctgtttc gccttgctgt actaataagc
      181 gaaagattgt taatcgagag tcttgagcga gtgccgcaag cagttgaacg gctgatttgt
      241 tttccatcgt ttttgccaaa ccaaaatttt agtaaatttt actatatttc tagaaatata
      301 gaaataaaac tcaagctagt tgtttaacaa aatatgctgt tttttaacca tttgtcgctc
      361 tttaagcgtc gtttatcgtg tgtgttggtt aaaagtaggg ttgttaggct gtttttataa
      421 aagtgtcatt aattacttgt agattgatct ttgccgataa ctggtttgga gggcatggtc
      481 atgagacatt tgttttttgt actattagct gtgtttagta tagctgttta tgcagataaa
      541 gaagacctac aaagcctaca aaaacaacaa aacgattggt atgtggtaaa acaggataac
      601 actcgtcata taacgactta tgccaaattt gaggatggca aaaaactgcg ttcctttaaa
      661 atagaagcaa cggttgatgc atcattagca actttagcaa gtattcattt tgatattgcg
      721 aatattaaaa aatggtattg ggaaaccttg gagtccaaat taattaacaa aatctcagat
      781 accgaatatt tatattatat gcaatttaac acccctttgg caccagatag ggatgtggtg
      841 attcatgcta aagttcaacc ttttcaagat gttattcacc cttatttgtt gttgcaatta
      901 cgtgctgccc catcagcatt gcctgtcccc aaaaacttta cccgtgtgag tgcatttgat
      961 atggatataa aatttacgcc attagttgat ggaaaaacta aaatggaagt acaaggctat
     1021 attgatcctt cgggtgagtt gcctgcatgg acagtcaatt tatttcagcg tcaagcacct
     1081 tattcgacga tgcttggctt atatagaatg attcaaaagc cagaatatac acataataaa
     1141 atacctttag cgtttactta tatgcgttga tggttttttc tggtgcaagc tctctaaatt
     1201 tagggagctt ttttgcaccc acaatcaaaa cgtcaatagc ccctatttaa aaaataaaaa
     1261 aatctaggtg ttatttgact ggctaagcta aaattacttt cttgaaattt tagcttgtac
     1321 cactgatttg tgggtatttt ttagactgga caccccatgt tacgcctcac cgaattacgt
     1381 ttacctattg atcatcaacc cgaagccatt aaaactgcga ttgctaaaaa acttgcgatt
     1441 tctgaggccg atattcaaca cattcatatc cataaacgta gttacgatgc gcgtaaagtc
     1501 agtcaaattg tcttaattta tattgtcgat attgacgtta acaatgaagc caaactatta
     1561 gccaaattca aaaatgatcc tcatgtacaa gcatcacctg atatggccta taagtttgtt
     1621 gcccaagcac caaacatgtt aacagagcga ccgattattg tcggttttgg cccgtgtggt
     1681 attttggcgg ctttagtctt ggcacaaatg ggttttaaac cgattgtgtt agaacgtggc
     1741 aaagaagtgc gccaacgcac caaagacaca tggggtttat ggcgtaaaaa tatcctcaat
     1801 cccgaatcga atgttcagtt tggtgaaggt ggggcaggca cgttttctga tggtaagtta
     1861 tatagccaaa tcaaagaccc ccgtcactta ggtcgtaaag tcttggatga atttgtcaaa
     1921 gcaggcgcac ctgaagaaat tttgtatatc agcaaaccgc atattggcac atttaaattg
     1981 gtaacgatgg tcgaaaccat tcgccaaacc attgagtctt taggtggaga aattcgcttt
     2041 cagcaaaaag tggttgatgt gttgattgat aatggccaaa tacgtggcgt gcgtttagaa
     2101 aatggtgaag agttgcatag ccgtcatgtg gtaatggctt taggccatag tgcgcgtgat
     2161 acttttacca tgttgcatga acgtggtgtg tatatggaag ccaaaccgtt ttctattggt
     2221 tttcgtatcg aacatccaca aggcatgatt gatcgttgtc gttttggcaa atttgcaggg
     2281 catcctttat tgggggcagc cgattataaa attgtacatc acgccaaaaa tggtcgtgcc
     2341 gtttatagtt tttgtatgtg tcctggtggc acagtggtgg cggcaacctc tgagcctaat
     2401 cgagtggtta ccaatggcat gagccaatat tcgcgtaatg aacgtaatgc caattctggc
     2461 cttgtagttg acattcagcc tagcgatttt ggcgatagtt caccacttgc aggtattgcc
     2521 tttcaacgca tttgggagtc aaaagccttt gaattgggcg gtggtactta tgctgcaccc
     2581 gcacaattag tgggagattt tttggcaggg cgtccatcaa ccaaggttgg aacagttgaa
     2641 ccttcttata aacctgcggt gaattggtgt gatttaagct cagccttacc tgagtatgtg
     2701 attacggcca tgcgtgaggc tttgcctgag tttgataaaa aactgaaagg ttatgccatg
     2761 catgatgcag tattaacagg ggttgaaacc cgtacctcat cgccattacg catcacgcgt
     2821 aacgagcatt accaaagctt gaatgtcaaa ggtttattcc ctgcgggcga aggagcaggc
     2881 tatgcaggcg gtattttgtc tgctggtgtt gatggcatta aagtggctga agccgttgct
     2941 ttgagtat
//
LOCUS       DAOQMB010000283         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19179_length_2942_cov_16.576030, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000283 DAOQMB010000000
VERSION     DAOQMB010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19179_length_2942_cov_16.576030"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2942
                     /gene="recC"
                     /locus_tag="PLY05_08855"
     CDS             <1..>2942
                     /gene="recC"
                     /locus_tag="PLY05_08855"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNC03448.1"
                     /translation="PASFMWECFGKIVPETPKSPILSPQAMHWRLFTALENLPNDEIY
                     QVLHRYLERNPQPLVGRWQLAGRIAEVFGYYRTYRRDWLALWRQGQLVDKPFRHQEWQ
                     AALWQQLFAEEHHQQGYLIKQFYWQLKNHPHLLKRLPQRLAIFTTVRLPPHELEFFRV
                     LSLFIDIHFYHFNPSGQYWADSVDEKWLAKMQVKQPTRMALYDKGHPLLTAWGKQHRD
                     TFRLLSQLSGGEQDNEWIDVFPEIPVKHLLSQLQDSIHRLDHIEEATWTIAEDDQSIQ
                     IHSCHSLTRQLEVLHDTLLHLFAQEPSLKPQDVVVMLPDIASASGAIEAVFGTVSKDR
                     YIPWQLTGVASVAENSLWRAFESLYSLSQERFALSTFIDWLSLAPVLSYYDLVAEDIE
                     RCVSLLEKAAVYRGLDGLHRAQMTGDNTDTDDVHTFLFGLERLLLATALPSDYQELYA
                     GIQPVADIEASDFALIGVLCQAMSDIAERRYAWQQRQHALQGLAQLQADLLKFFVAQV
                     GSRAWESLTKAIEELQETLTISPINEPIPLSLLLSDLQQRVAASAPGAVPGGVVHFSR
                     LGSLRLLPYKVIAMLGLEDTAFPRREQNNEFDLLSQDDQPRAGDRSRRDDDKAMFLEN
                     LMTAEEHLLLFYNGFSHHSATHYPPSSLISQLLDYLSRRVIGGKTEVEQHCCYFHRLQ
                     AFSPSYFGALSDKKLPISFAQEWLPAAQAVLTTSVVPSCFVNTSLPLDETLKQVSLVS
                     LYRFYQHPAKYFLSQQAKLHIQTLQDQHRDEEPLRLDNLAKWQLRSQLVHEQQLPLAR
                     FRASGQLPVGVIGEVYCQQSAQDLQQLKQAIQRYQLDYQKQHYLFTLLDDNQHSWQLS
                     AHLAPVDLPYQLEYIVSKCRDKHLLNAWLHHLLWQLAGANRPTYWLFLDQAYCFQPIP
                     RLQAEHYVQVYYRYLQQGLQQPLLLSVEAAFSYMTKLNGKQADDAAKAALKIWSAQAQ
                     EDVYWRQV"
BASE COUNT          821 a          592 c          642 g          887 t
ORIGIN      
        1 cctgccagtt ttatgtggga atgttttggc aaaattgtcc ccgaaacacc aaaatcgcca
       61 attcttagtc ctcaagcgat gcactggcga ttatttactg ccttagaaaa tttgcctaat
      121 gacgaaattt atcaagtctt acatcgttat ttagagcgta atcctcaacc tttagtgggg
      181 cgttggcaat tagcagggcg cattgctgaa gtatttggtt attatcgtac ctatcgacgt
      241 gattggttgg ctttgtggcg acaagggcaa ttagttgata aaccttttcg tcatcaagaa
      301 tggcaagccg ccttatggca acaattattt gccgaagaac atcatcaaca aggctattta
      361 attaaacaat tttattggca gttaaaaaat catccgcatt tgttaaaacg cttgccacaa
      421 cgcttggcga tttttactac agtacgtttg ccaccccacg aattagagtt ttttcgggtg
      481 ttaagtctgt ttattgatat tcatttttat cattttaacc ccagtggcca atattgggcc
      541 gacagtgttg acgaaaaatg gctggctaaa atgcaggtta aacaaccgac acggatggct
      601 ttatatgata agggacatcc gctattaacg gcatggggca aacaacatcg cgataccttt
      661 agattattgt cgcaattgtc gggtggtgag caagataatg aatggattga tgtttttcct
      721 gaaattccag ttaaacatct gttatcacag ctacaagata gcattcatcg tttagatcat
      781 attgaagagg ccacatggac aattgccgaa gacgatcaat ccattcaaat tcatagttgc
      841 catagtctta cccgacaatt agaagtctta cacgatacct tattacattt atttgcccaa
      901 gaacccagct taaaaccaca agatgtggtg gtgatgttgc ccgatattgc cagtgccagt
      961 ggtgcaatcg aagcggtgtt tggcacagtc agcaaagatc gttatattcc ttggcagtta
     1021 actggggtgg ctagtgttgc cgaaaatagc ttgtggcggg cgtttgaaag tttatacagc
     1081 ctaagccaag agcgttttgc tttatcaacg tttattgatt ggctgagttt agcgcctgtg
     1141 ttgagttatt atgatttagt ggcagaagac atcgaacgtt gtgtcagctt attagaaaaa
     1201 gccgccgtgt atcgtggttt ggatggtctg catcgtgcac aaatgacagg tgataatact
     1261 gatacggatg atgtgcatac ctttttattt ggtttagagc gcttattatt agcaactgcc
     1321 ttacccagtg actatcaaga gctgtatgca ggcattcaac ctgttgctga tattgaggcg
     1381 agtgattttg ccttgattgg ggttttgtgc caagcgatga gtgatattgc cgaacgccgc
     1441 tacgcatggc aacaacgtca gcacgcctta caaggtttgg cacaacttca ggcagatttg
     1501 ttaaagtttt ttgttgctca ggttggttcg agagcatggg aaagcttaac caaagccatc
     1561 gaagagctgc aagaaacctt aaccatttca cccatcaatg aacccatacc tttaagttta
     1621 ttattatctg atttacaaca acgtgttgcg gcttctgcac ctggtgctgt accgggtggc
     1681 gtcgtgcatt ttagccgttt aggctcatta cgtttattgc cctataaagt catcgccatg
     1741 ttgggcttag aggacaccgc ctttccgcgt cgggaacaaa ataatgaatt tgacttgtta
     1801 agccaagatg accaaccaag ggcaggtgac cgttcacggc gtgatgatga caaagcgatg
     1861 tttttagaaa acttaatgac tgccgaagaa catttgttgt tgttttataa tggttttagt
     1921 catcacagtg ccacgcatta cccgccatca tcgttaatta gccaattatt agattattta
     1981 agtcggcgag tgattggcgg caaaactgag gtcgaacaac attgttgcta ttttcatcgt
     2041 ttacaagcct ttagtccgag ttattttggt gcgttgagtg ataaaaagtt gcccatcagt
     2101 tttgcccaag aatggttgcc tgcggcacaa gcagtgttaa ccaccagtgt tgtgccttcg
     2161 tgttttgtca atacgtcatt gcctttggat gaaactttaa aacaagtgag ccttgtcagc
     2221 ctatatcgtt tttaccaaca tcccgctaaa tattttttaa gtcaacaagc caaactgcat
     2281 attcaaactt tacaagacca acatcgagac gaagaaccct tacgtttaga taatttggcc
     2341 aaatggcaat tacgcagcca attggtgcat gagcaacaat tgcctttggc gcgatttcgt
     2401 gccagtggcc aattgcctgt tggggtgatt ggtgaagtgt attgccaaca aagtgcacaa
     2461 gatttacaac aactgaaaca agcgattcaa cgctatcagt tggattatca aaagcagcac
     2521 tatttattta ctttattgga tgataatcag cattcatggc aattaagcgc gcatcttgcc
     2581 cctgtcgatt taccgtatca attggaatat attgtttcta aatgccgaga taaacattta
     2641 ttaaatgcgt ggttgcatca tttattatgg caattagcag gcgcaaatcg accaacctat
     2701 tggcttttct tagaccaagc ctattgtttt caaccgattc cacgcttaca agccgaacat
     2761 tatgtgcaag tttattaccg ctatttacaa caaggtctac aacaaccgtt attgttaagt
     2821 gtcgaagccg cttttagcta tatgacgaaa ttgaatggca aacaagcgga tgatgccgcc
     2881 aaagctgctc taaaaatatg gagcgcacaa gcccaagaag atgtgtattg gcgacaagtg
     2941 tt
//
LOCUS       DAOQMB010000284         2942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19181_length_2942_cov_6.281261, whole genome shotgun sequence.
ACCESSION   DAOQMB010000284 DAOQMB010000000
VERSION     DAOQMB010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19181_length_2942_cov_6.281261"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLY05_08860"
     CDS             <1..374
                     /locus_tag="PLY05_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HNC03449.1"
                     /translation="LLGSLELISSTAKSQYRFNIGTHESLHDAVFAFALLDFWDRWEQ
                     ETGASQRTLSFSAVAHWYNSPGRVFKLSEDAVADRLSRIAEITNNYLTWTDQTGIRQV
                     TRVGSTPLEESKMAILRGAYV"
     gene            367..>2942
                     /locus_tag="PLY05_08865"
     CDS             367..>2942
                     /locus_tag="PLY05_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC03450.1"
                     /translation="MSKTLSDVIDIKRRFLKSINLNLDFGRYDAIDGYILQSSTQKLL
                     HTLGHHLTHTQQKAFTLTGPYGTGKSLLALTIASLAGGTNILRNQAKAILSNDDTNAF
                     VQYFSTDKPWLVLPITGKRSSIDQELKLAISTAIPETEKSKKSVIKQLKHITETNSEY
                     EGVLLIIDELGKFLEYAAYSGEDVGFYQELAEMAARSKGKLVILGILHQAFEQYASRL
                     GKETQQEWAKVQGRYVDIPLSTTTDETLYLLSKAIEVNSTYSKHLNSAESIAQFIPNQ
                     QQTNIASVLKDCWPLHALTALMLGPSSRKRYGQNERSIFSFLASAEPLGFYEITTSLV
                     ANQEHSLYWPHHYWDYLRANFEPTILVSSDSHRWSLTLDALQRAESLYPVLHLQIIKT
                     LGLLDLFRSSLGLYAKPELLYASIPATKNEITQALQELSQSSILIFRKHTESWAIYAG
                     SDFDIEEAIQQAKHELGALNLSQIDNILELNPVIARRHYAEKGAMRWFDKKFVLEQDV
                     SKHLKSFTINGSKCGEFLLVLQTHSQSLASIATLQKELSVQAPQGVVIGISNEAETLI
                     QKLEDLRSLDFIRKHRLELHTDQVAMREVDARIHAIRLSLGDTIQNLFNQAQWMYQDK
                     LQSGNLTQIASEIANHIYSDSPIILSELVNRNHLSANAIKAQKDLLRAMLSSSHLENF
                     GFESMSAPAGLYRTTLKTLGMHNSIDNIWHITKPNGSSPKSASAVNLWQAAEDFIFKS
                     QQSTPLNILYQLWTQAPYGIKKGLLPIFALAFYLANRKHLALYMDGVFTPDLTEATLD
                     EWLQDPKRVSWRFVKLDSSEQDILHTLATTLSKQLNKKISEDPLDSARALVSIVYSIP
                     MWT"
BASE COUNT          951 a          574 c          519 g          898 t
ORIGIN      
        1 cactgcttgg ttctttagag ttaatatcaa gcacagctaa aagccaatat cgttttaata
       61 ttggcactca tgaatcatta catgatgccg tgtttgcatt tgcattatta gatttttggg
      121 atcgctggga gcaagaaaca ggagcctctc aacgaacttt atcttttagt gctgttgctc
      181 attggtataa ctcgcctgga cgtgtattca aactatcaga agatgctgtt gctgatcgct
      241 tatctcgcat tgctgaaatt acaaataatt atcttacatg gactgatcaa actgggattc
      301 gccaagttac tagagttggc tcgacaccat tagaggaaag taaaatggct attttaagag
      361 gtgcttatgt ctaaaacttt gtcagatgtc attgatatta aaagacgctt cttaaaatct
      421 attaatttaa atttagactt tggacgttat gatgcaattg atgggtatat attacaatca
      481 tcaacgcaaa agctattaca tacgcttgga catcatctca ctcacactca acaaaaagca
      541 tttacattga cagggcctta cggtactggc aaatcattac ttgcattgac catagcatct
      601 cttgcaggtg gtactaatat tcttcgtaat caagcaaaag cgatattatc caacgatgac
      661 acaaatgctt ttgttcaata tttttctact gataaaccgt ggcttgtgtt accaatcaca
      721 ggtaaacgca gcagtatcga tcaagaactc aagttggcga ttagcacagc aatacccgaa
      781 actgaaaaat ctaaaaaaag tgtaatcaag cagcttaaac atataacgga aacaaattca
      841 gaatacgaag gtgtactact cattattgat gaattgggta aatttttaga gtacgctgca
      901 tattcaggtg aagatgttgg cttttaccaa gagcttgctg aaatggcagc tcgctctaaa
      961 ggcaaacttg ttattttagg catactacat caagcatttg agcaatatgc ctcacgtttg
     1021 ggtaaagaga ctcaacaaga atgggcaaaa gtacaaggcc gatatgtcga tataccgtta
     1081 agcactacga ctgacgaaac tctttactta ctttctaaag ctattgaagt aaacagtaca
     1141 tatagcaaac atcttaattc ggcagagtct attgctcaat ttattcccaa tcaacaacag
     1201 actaatattg cctcagtact caaagattgc tggccactac atgctttaac ggcattaatg
     1261 cttggcccct cctcacgtaa aagatatggg caaaatgaac gcagcatatt cagcttttta
     1321 gcatcagcag agcctttggg cttttatgaa atcactacat ctttagttgc taatcaagaa
     1381 catagccttt attggccgca tcattattgg gactatttaa gagctaattt tgaacctacg
     1441 attttagtat catcagatag ccatcgatgg tctttaacct tagatgcctt acaacgtgca
     1501 gaatccctct accctgtact acatcttcaa attattaaaa cgcttggatt acttgattta
     1561 tttcgcagca gtcttggctt atatgctaag cctgaattac tttatgcaag tattcctgca
     1621 actaaaaatg agattactca agccttacaa gaattatctc aatcatcaat tttgattttt
     1681 agaaaacaca cagagtcttg ggctatttat gcgggtagtg actttgatat tgaagaagcc
     1741 atacagcaag ccaaacatga acttggagca cttaatctaa gtcaaattga taatatttta
     1801 gagttaaacc ctgttatcgc acgtcgtcac tatgcagaaa aaggtgcaat gcgttggttt
     1861 gataaaaaat ttgtccttga acaagatgta agcaaacact taaaaagctt tacaatcaat
     1921 ggcagtaaat gtggtgaatt tctattagta ttacaaacac acagtcaatc attagctagt
     1981 atagctacgc tacaaaaaga gctttcagtt caagcacctc aaggtgtggt aattggtatc
     2041 tctaacgaag cagaaacgtt aatacaaaaa ctagaagact taagaagctt agattttatt
     2101 cgtaaacatc gtttagagtt acatacagac caagttgcca tgcgtgaagt agatgcaagg
     2161 attcatgcta ttaggctgtc tttgggggat acaatccaaa acttatttaa tcaagctcaa
     2221 tggatgtatc aagataagtt gcaatctggc aacctaaccc aaattgcctc tgaaattgct
     2281 aatcatatct actctgactc ccctattatt ttaagtgagt tggtcaatcg taatcatctt
     2341 tctgccaatg cgattaaggc acaaaaagac ttacttcgtg ccatgctctc aagtagtcat
     2401 ttagaaaact ttggttttga aagcatgtct gcgccagcag gcttgtatcg tactacctta
     2461 aaaacattag gcatgcacaa tagcatagac aatatttggc acattactaa acctaatggc
     2521 tcatccccca aatcagcaag tgctgttaat ctttggcaag ctgctgaaga ttttattttt
     2581 aaatctcaac aatctacgcc gttaaatatt ttatatcaat tatggactca ggctccttat
     2641 ggtattaaaa aaggcctact acctattttt gctttagctt tctatcttgc taacagaaaa
     2701 catttagcac tctatatgga tggggtattc acaccagacc tgacagaagc aacgcttgat
     2761 gaatggttgc aagaccctaa aagagtttca tggcgttttg ttaaacttga ctctagtgag
     2821 caagatattt tacacactct tgctacgaca ctaagtaagc aactaaacaa aaaaattagc
     2881 gaagacccat tagattctgc acgcgctctt gtttctattg tctattctat tcccatgtgg
     2941 ac
//
LOCUS       DAOQMB010000285         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19226_length_2938_cov_18.398890, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000285 DAOQMB010000000
VERSION     DAOQMB010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19226_length_2938_cov_18.398890"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..842)
                     /locus_tag="PLY05_08870"
     CDS             complement(39..842)
                     /locus_tag="PLY05_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HNC03451.1"
                     /translation="MANFIKYAVIGGIALVLVLILNPFVVIGAGQRGVVLNFGAVQKE
                     VLDEGLHFRVPIMQKVVIMNVQVQKGEGQGDAASKDLQQVTTNVAVNYHLDPQKVAQV
                     YQKIGTFDAVGDRIILPAVSESVKAATAQYTAEELISKRQEVREKIRQHLLTRLAVYG
                     VIVDDFSIVNFSFSQEFNNAIESKTTAEQLKLKAERDLERIKIEAEQKITQAQAEAES
                     LRLQKENVTENLIKLRQIEMQQRAIEKWDGKLPQVTGGATPFIDLRQGQ"
     gene            919..2859
                     /gene="dsbD"
                     /locus_tag="PLY05_08875"
     CDS             919..2859
                     /gene="dsbD"
                     /locus_tag="PLY05_08875"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HNC03452.1"
                     /translation="MMLFLTSLTRAEELKSFAGGASFSSSGNKTQFLKAEQAFMLQYE
                     QKGQELILNFTIAPNYYLYKERFKFQVNNGNAILGQAYYSLEPEWKDDAEFGRVQVFH
                     QSLSITMPVKGNGEILVRWQGCADAGLCYAPQEQLISLAGAPSSSPATLINNTTVNRE
                     SVSIPIPTLIPLVSYDIVEEETPVNENIITETPNSDNAKLITKNTATLILPSTTDSTT
                     TIPTTTSTINNDFKLPENPMLALLTLFVLGLGLAFTPCVLPMLPIVANIVARQHASNA
                     RQGLFLGFAYAIGVASSYGLLGVLVSAFGSQVNLAAWLQHPSILISFSVLFIILALAS
                     FDVFHLQLPQSWQHRLDQLSKTGKAGSFIGTWLTGFFSALVVSPCVSAPLAGVLLSVS
                     TLGNPLMGGIALFMLGLGLSTPLMILAASEGKFMPKAGLWLNRVKQTFGVLLLAVAVA
                     LLSRVITDASMLFLWAALCVGSGFWINQWGGKWRVVWRSIAYMLLIWGATLLVGAALG
                     SNDPVKPLAIIKNNTVANFSSPSTEKHTIHSVDELQQYVAQAKQENKILLVDFYADWC
                     VSCKIMEREIFNQADVQNQLQNWLIVKADVTQNSATERMLQQQLAVFGPPALVFFKDG
                     QEVSRLVGEVSKSQFLEHISSM"
BASE COUNT          840 a          552 c          576 g          970 t
ORIGIN      
        1 gggggttttc tttgttagaa gttagtactg actcaaaact attgtccttg tcttaaatca
       61 ataaacgggg ttgcaccacc tgtcacttgt ggcaatttac catcccattt ttcaatagca
      121 cgttgttgca tttcaatttg acgtaattta atcaaattct cagtcacatt ttctttttgc
      181 aatcgcaaag actccgcttc agcttgagct tgagtaattt tttgttcggc ttcaatttta
      241 atccgttcta aatcgcgctc agcttttaac ttcaactgtt cggcagtggt tttagattca
      301 atcgcattat taaactcttg agaaaacgaa aaattaacaa tcgaaaaatc atcaacaata
      361 acgccataaa ccgctaaacg agttaataaa tgttgacgta ttttttcacg gacttcttga
      421 cgtttagaaa tcaattcttc ggcggtatat tgagcagtcg ctgctttcac agactccgaa
      481 accgcaggca aaataattct gtcaccgaca gcatcaaatg tgccaatttt ttgatatacc
      541 tgagccactt tttgtgggtc taaatgatag ttaactgcca cattggtggt gacttgttgc
      601 aaatctttag aggcggcatc accttgtcct tcaccttttt gcacttgcac attcataatc
      661 accacttttt gcataattgg cacacgaaaa tgcaagcctt catctaaaac ttctttttgc
      721 actgcgccaa aattaagcac aacacctcgt tgaccagccc caatgaccac aaaagggttt
      781 aaaattaaca caagcactaa tgcaataccg ccaataaccg cgtattttat aaaatttgcc
      841 atttgcccaa ctcctgttga tttttgagcg ataatttaga tgaccgtaaa caagcttatc
      901 acgatagttt tgacttttat gatgttattt ttaacgtcgc tgacaagggc tgaagaatta
      961 aagagtttcg ctggtggcgc atcgtttagt agtagtggca acaaaacaca atttttaaaa
     1021 gccgaacaag cttttatgtt gcaatacgaa caaaaaggcc aagagttaat tcttaatttt
     1081 accattgcgc ctaattatta tttatacaaa gaacgtttta aatttcaggt caataatggc
     1141 aatgccattt taggccaagc ttattatagc cttgagccag aatggaaaga tgatgcagaa
     1201 tttggccgag tacaagtttt tcatcaaagc ctaagcatta ctatgcctgt taaaggtaat
     1261 ggcgaaattt tagttcgttg gcaaggatgt gctgatgcgg gtttatgtta tgccccacaa
     1321 gagcaactaa ttagtctggc tggcgcacct agtagtagcc ctgctacctt aattaataat
     1381 acgactgtta atcgagaatc ggtcagtatt cctattccta cactgattcc attagttagc
     1441 tatgatattg ttgaagaaga aacgcctgtt aacgaaaaca ttataactga aacacccaac
     1501 tcagataatg ccaagctgat taccaaaaat acagccacct taattttgcc atcaacgacc
     1561 gactcaacaa caactattcc gacaacgact tcaaccatta ataatgattt taaattacct
     1621 gaaaatccaa tgttggcctt gttaacacta tttgttttag gcttaggttt agcatttacc
     1681 ccttgcgtat tacccatgtt acccattgtt gctaatatcg ttgcgaggca acatgctagc
     1741 aatgctcgac aaggtttatt tttaggtttt gcttatgcca ttggcgtggc tagtagctat
     1801 ggtttgttag gtgttttggt gtctgctttt ggcagtcaag ttaatttagc ggcttggcta
     1861 caacatcctt ctattttaat tagttttagt gttttattta ttattttggc tttggctagt
     1921 tttgatgtat ttcatttgca actgccacaa agttggcagc atcgtttaga ccaactgagc
     1981 aaaacaggca aagcaggtag ttttattggc acatggttaa caggcttttt ctcggctttg
     2041 gttgtttctc cgtgtgtatc agcaccatta gcgggtgttt tattatctgt ttcaacgctg
     2101 ggcaatccac taatgggtgg catagcgttg tttatgttgg gtttaggttt aagcacacct
     2161 ttaatgattt tagccgccag tgaaggcaaa tttatgccta aagcaggttt atggcttaat
     2221 cgcgtcaaac aaacctttgg tgtgttatta cttgctgttg cggttgcttt gttatcacga
     2281 gtgattacag acgctagtat gttattttta tgggcggctt tatgtgtagg cagtggattt
     2341 tggattaacc aatggggtgg caaatggcgt gtggtatggc gcagtattgc ctatatgtta
     2401 ttaatttggg gcgcgacttt attggttggc gcggctttag gcagcaatga ccctgttaaa
     2461 ccattggcaa ttatcaaaaa taatactgtc gctaattttt catcaccatc cacagaaaaa
     2521 cataccattc atagtgttga tgaattacaa caatatgtcg cccaagctaa gcaagaaaat
     2581 aaaatattgt tggtagattt ttatgcagat tggtgtgtta gctgcaaaat tatggaaaga
     2641 gaaattttta atcaagcaga tgtacaaaat caattacaaa actggttaat tgttaaagct
     2701 gatgtgactc aaaatagtgc gactgaacga atgctacaac aacaattagc ggtttttggc
     2761 cctcctgctt tagtcttttt taaagatggg caggaggtct ctcgtttggt gggtgaagtc
     2821 tcaaaaagtc aatttttaga gcatatcagt tcgatgtgag aggttggcaa acgcataagt
     2881 ttaaggaagt ttgctggttt ttctcgtctg ctaacaagca ttgttgattt agtatcaa
//
LOCUS       DAOQMB010000286         2938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19227_length_2938_cov_14.660076, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000286 DAOQMB010000000
VERSION     DAOQMB010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19227_length_2938_cov_14.660076"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLY05_08880"
     CDS             <1..513
                     /locus_tag="PLY05_08880"
                     /inference="COORDINATES: protein motif:HMM:NF019473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC03453.1"
                     /translation="TITTLGETSMTAQQNQQLVDNFYKAFQQKDGEAMAKSYADNATF
                     SDPAFSNLQGQQVGDMWRMLTERAQNFSLQYKVLTVGEQQGEAEWTATYTFSQTGRTV
                     VNHIHSYFTFKDGKIIAQQDKFDVWRWSSQALGLKGTLLGWTPLVKNKIQQQASKSLA
                     IYQQQKNKAK"
     gene            complement(568..>1621)
                     /locus_tag="PLY05_08885"
     CDS             complement(568..>1621)
                     /locus_tag="PLY05_08885"
                     /inference="COORDINATES: protein motif:HMM:NF019379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1615 domain-containing protein"
                     /protein_id="HNC03454.1"
                     /translation="PVLVPTTTPTKTNGISVDEARLLLQSLLPIGIKDRNGWRDDILS
                     AFTGLKIPYEAQYFCGALAIIEQESSWQSDPTVANLNKIVWQEIEKRADKYHIPMFAV
                     KLALLKPSPDGRSYNARIDALRTEKQMNALFEDIVSDAQKIGLPFSMKNPIRTGGPMQ
                     VSVEFAEAHSKAWPYPYSYKGSLRQEVFSRRGGLYFGIANLLQYRANYPQMKYRFADF
                     NAGRYSSRNAAFQAAVSTLSKKKLVLDGDLLVYTSNKASSTQQQLVKLAPRLGLSAKA
                     IERDLQLEKSEAFYQTALFKKVFELANQQTGRVLPQQVMPQIKLVSPKITRKLTTEWF
                     AERVDGRYQTCMNKQK"
     assembly_gap    1622..1721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1796..>2938
                     /locus_tag="PLY05_08890"
     CDS             1796..>2938
                     /locus_tag="PLY05_08890"
                     /inference="COORDINATES: protein motif:HMM:NF039651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC03455.1"
                     /translation="MNNKSKYYMAASALMAASIATAAPTASTKPVVAGSILSRVQAFL
                     DKPATWDKIPKNVTLCMFAPGGIQGKTFQYVRNNMSELTKYTAIAKDIGIDFNVTMTS
                     PTNMRIDLASSKLKRKVSTDVKFNIYNDERVASEDFKVGQCDGIAMSNIRARQYNQFV
                     GSLDAIGAVLSYKQLGEVLNILAKPEMNKYMVSQNYEIVGVIPMGAAYIMVNDRKINT
                     ISKAAGKKVAVFDFDKSQAKMVQNIGAQPISVDFSNVGGKFNNGQVEIMAAPALAFEP
                     LELYRGMTDAQGNARGAIIKFPLLQVTGVLMMYQNKFPDGLGPLIREFISGQLAPAYQ
                     FVLETENEIPSKYWMDLPEADKPGYVAMMRTARIQMTKEGHYDKQMM"
BASE COUNT          853 a          542 c          604 g          839 t
ORIGIN      
        1 accatcacaa cattagggga aacatctatg actgctcaac aaaatcaaca attagtggat
       61 aatttttata aagcgtttca gcaaaaagat ggcgaagcca tggcaaaaag ttatgccgat
      121 aatgcgacgt ttagcgaccc tgcatttagc aatttacaag gacaacaagt aggggatatg
      181 tggcgaatgt tgactgagcg tgctcaaaac ttttcattac aatacaaagt gttaacagtg
      241 ggtgagcaac aaggcgaagc agagtggaca gcaacttata ccttttcgca aactggccga
      301 accgtggtta atcatattca cagctatttt acgtttaaag atggcaaaat tatcgcccaa
      361 caagataaat ttgatgtgtg gcgttggtct agccaagcgt taggtttaaa aggcacattg
      421 ttgggttgga cacctttagt gaaaaataaa attcaacaac aagcgtctaa atcgttggct
      481 atctatcaac aacaaaaaaa taaagcaaaa taatagtgtc acaattttcc ctctcctgtt
      541 aggagagggt tagggtgagg tctgatttta cttctgttta ttcatacagg tttgataacg
      601 accatctaca cgctcagcaa accattctgt ggtcagtttg cgggtaattt ttggactaac
      661 caatttaatt tgtggcatca cttgttgagg caaaactctg ccagtttgtt gattggctaa
      721 ttcaaatact tttttaaaca gggcagtttg ataaaaagct tcgctttttt ctaattgcaa
      781 atctcgttca atcgctttag cagataaacc caaacgtgga gcaagtttca ctaattgttg
      841 ttgagtgcta ctggctttgt tgctggtgta caccaataag tcaccatcca acaccaattt
      901 ctttttgctt aatgtgctca cagctgcctg aaaagctgca ttacgactgc tatatcgtcc
      961 tgcattaaaa tcggcaaaac ggtatttcat ttgtgggtag ttggcgcgat attgcaataa
     1021 attagcaata ccaaaatata aaccaccacg acggctaaac acttcttggc gcaaactgcc
     1081 tttataacta tagggatatg gccacgcttt gctgtgtgct tcggcaaact ctacgctaac
     1141 ttgcatgggc ccacctgtac gaatggggtt tttcatcgaa aagggtaaac caattttttg
     1201 ggcatcactg acaatatcct caaacaaagc attcatttgt ttttcggttc ttagcgcatc
     1261 aatccgcgca ttataactac ggccatcagg tgaaggtttt aataacgcca atttaacggc
     1321 aaacatcgga atatgatatt tatcggcacg tttttcgatt tcttgccaaa caattttatt
     1381 taaattagcc acggttggat cactttgcca actcgactct tgttcaataa tcgctaatgc
     1441 cccacaaaaa tattgtgctt catagggaat ttttaaacct gtaaaggccg ataaaatatc
     1501 atcacgccag ccgttacggt ctttaatgcc tataggtaat aaactttgta ataacaatcg
     1561 tgcttcgtca acactgatgc cattggtttt agttggtgtt gttgttggca caagcacagg
     1621 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natatgatga ttgtcactat
     1741 tttattgtct gttcaccgat tacattgatt gcatcaacgt ttgggggata caacaatgaa
     1801 caacaaaagc aaatattata tggcagcttc ggcattaatg gcggcaagta ttgccacggc
     1861 tgcacccaca gcaagcacaa aacctgtggt tgcaggttct attttaagcc gagtacaagc
     1921 ctttttggat aaacccgcaa catgggacaa aatccctaaa aatgtgacct tatgtatgtt
     1981 tgcaccgggt ggcatacaag gcaaaacttt tcagtatgtt cgcaacaata tgtcggagtt
     2041 aaccaaatac accgccattg ctaaagacat tggtattgat tttaacgtta ctatgacttc
     2101 gccaaccaat atgcgtattg atttagcctc aagcaaatta aagcgcaaag tcagtacaga
     2161 cgtaaagttt aatatttata acgatgaacg tgttgcctcc gaagatttta aagttggtca
     2221 gtgtgatggt attgccatga gtaatattcg cgcacggcaa tataatcagt ttgttggtag
     2281 tttggatgcg attggcgcag tgttgtctta taaacagtta ggcgaagtat taaatatttt
     2341 ggccaaaccc gaaatgaata aatatatggt tagccaaaat tatgaaattg tcggtgtgat
     2401 tccgatgggc gcggcctata ttatggttaa tgaccgcaaa attaacacca tttctaaggc
     2461 agcaggcaaa aaggtggcgg tgtttgattt tgataaatca caagcaaaaa tggtgcaaaa
     2521 cattggcgca caacccattt cggtcgattt ttctaatgtt ggcggaaagt ttaacaatgg
     2581 ccaagtagag attatggctg ctcctgcctt ggcgtttgag cctttagagt tgtatcgtgg
     2641 tatgaccgat gcacaaggca atgcgcgtgg cgcgattatt aagtttccct tgttgcaggt
     2701 gacgggtgtg ttgatgatgt atcaaaacaa gtttcctgat ggtttagggc cattgattcg
     2761 tgagtttatt tcgggacaac ttgcgccagc ctatcagttt gttttagaga cagaaaacga
     2821 gattccgtct aaatattgga tggatttgcc cgaagccgat aaaccaggtt atgtggcgat
     2881 gatgcgtacg gcgcggattc agatgactaa agaaggtcat tacgataaac aaatgatg
//
LOCUS       DAOQMB010000287         2917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19468_length_2917_cov_13.659679, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000287 DAOQMB010000000
VERSION     DAOQMB010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19468_length_2917_cov_13.659679"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..79)
                     /locus_tag="PLY05_08895"
     tRNA            complement(4..79)
                     /locus_tag="PLY05_08895"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44..46),aa:Asn,seq:gtt)
     gene            complement(255..1439)
                     /locus_tag="PLY05_08900"
     CDS             complement(255..1439)
                     /locus_tag="PLY05_08900"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNC03456.1"
                     /translation="MELRLSDRVLSIKPSPTLAVTNRAAELKAAGKDIIGLGAGEPDF
                     DTPEHIKAAAIKAIQDGFTKYTAVDGTAGLKKAIIAKLKRDNGLDYQANQILVSCGGK
                     QSFFNLALAFLNKGDEVIIPAPYWVSYPDMVLIADATPVCIECPQSQNYKITPAQLEA
                     AITPNTKLLVLNSPSNPTGMVYSKEELLALAEVLRKHPHVWVATDDMYEHIIWTKEPF
                     VNIVTVAPDLYDRTLVLNGVSKAYAMTGWRIGYAAGPAKVIGAMKNVQSQSTSNPTSV
                     SQVAAEAALNGDQDVLKPMVAAFKERHDYVVGALNQIAGVSCSPADGAFYAFANVEGA
                     IAKMGLKNDLEFSEVLLNTVGVAVVPGSAFGLDGHMRISYATSLKTLEDAIARIKKAI
                     EG"
     gene            1583..>2917
                     /locus_tag="PLY05_08905"
     CDS             1583..>2917
                     /locus_tag="PLY05_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC03457.1"
                     /translation="MQTKQAFQLQTEFQPSGDQPTAIQALCDSIQQGKQHQLLLGVTG
                     SGKTFTIANVIAKLQRPTIIMAHNKTLAAQLYSEFKSFFPNNAVEYFVSFYDYYQPEA
                     YVPSSDTYIEKDSAINEHIDQMRLSATKALLERRDAIIICSVSAIYGLGDPDAYLKML
                     LHVSRGERIDQRAILRRLAEMQYQRNDLEFSRGTYRVRGEVIDVFPAESDDEAIRISL
                     FDDEVEGIHYFDPLTGAPLRKAARVTIYPKTHYVTPKEQIEQALGTIRAELEPRLQWF
                     MGENKLIEAQRLKERTQYDLEMLQQLGYCNGIENYSRHLSGRKEGEAPPTLFDYVPAD
                     ALLVIDESHVTVPQIGAMYKGDRARKENLVNYGFRLPSALDNRPMKFEEWEKISPQTI
                     FVSATPANYEQEHCPIAIEQVVRPTGLVDPDIEIRPAFTQVDDLMSEINARIL"
BASE COUNT          875 a          578 c          616 g          848 t
ORIGIN      
        1 atatggctcc ccaacctgga ctcgaaccag ggacacacgg attaacagtc cgttgctcta
       61 ccgactgagc tattggggaa cgtctgtcgg tgtggacgct atattagctt aaatttaaaa
      121 aaggtcaata ctttgaatgg tttgattgct taattgttgt gcaaaatgtg tgattctgct
      181 tattttttaa taattattaa ataacatatg caaaagtcct ctcctattac taggagagga
      241 ggcgcaatta aagcttagcc ttcaattgcc tttttgatac gagcaatcgc atcttctaag
      301 gtttttaagc tagttgcata agaaatacgc atgtggccat ctaaaccaaa agcagaacca
      361 ggtacaacag caacaccaac ggtatttaat aatacttcag aaaattctaa atcgttcttt
      421 aaacccattt tggcaattgc accttcaaca ttagcaaagg catagaatgc accatcagca
      481 ggtgagcaag aaacaccagc gatttggttt aatgcaccaa ccacatagtc atggcgttct
      541 ttaaatgcag caaccattgg ctttaatacg tcttgatcgc catttaaagc agcttctgcg
      601 gcaacttgag acacagaagt tgggttagat gtagattgag attgtacgtt tttcattgca
      661 ccaatgactt ttgctgggcc agcagcataa ccaatacgcc agccagtcat agcataggct
      721 ttagatacgc catttaacac taaagtacgg tcatataaat ctggtgcaac agtgacgata
      781 tttacaaatg gctctttagt ccaaatgata tgttcgtaca tgtcgtcagt ggcgacccaa
      841 acgtgaggat gtttgcgtaa aacctcagcc aaagccaata attcttcttt agaataaacc
      901 atacctgttg ggttagaagg tgagtttaag actaataatt tggtgtttgg tgtaatcgct
      961 gcttctaatt gagcaggggt gattttatag ttttgggatt gtggacattc gatacaaaca
     1021 ggtgttgcat ctgcaatcaa taccatgtct ggataagaaa cccagtatgg tgcaggaata
     1081 atgacttcat cacctttgtt taaaaaggct aaggctaagt tgaagaagct ttgtttgcca
     1141 ccacaagaaa ccaaaatttg attcgcttga taatctaaac cattatcacg ctttaattta
     1201 gcaataattg cttttttaag accagctgta ccatcaacag ccgtgtattt ggtaaagcca
     1261 tcttgaattg ctttaatagc agccgctttg atatgttctg gggtatcaaa atctggttcg
     1321 ccagcaccca aaccaataat gtctttacct gccgccttta attcagccgc acggttagtt
     1381 accgctaaag taggagaagg tttgatgctt aatacacggt cagataagcg tagttccacg
     1441 gaattaacct cttgtttatg ttaagacagc ttacaacgat aggtttaacg tcataaaatg
     1501 acgttacaat aaccttgtca gtatgcaagt tttaaattgg gcgtaataat accgcaaaat
     1561 atcgtcagat aagagaccta aaatgcaaac aaaacaggct tttcaattac aaaccgaatt
     1621 tcagccttct ggcgatcaac caacagccat tcaagccttg tgcgatagca ttcaacaggg
     1681 caagcaacat caattgttat taggggtcac aggctcggga aaaaccttca ctattgccaa
     1741 tgtcattgcc aaattgcaac gaccgaccat tattatggcg cataacaaaa ccttagccgc
     1801 gcagttgtat agtgaattta aaagcttttt ccccaataac gccgttgagt attttgtgtc
     1861 tttttatgat tactatcagc ctgaagctta tgtgccgtca tctgatactt atatcgaaaa
     1921 agattccgct attaatgaac acattgacca aatgcgcttg tctgccacta aagccctgtt
     1981 agagcggcgt gatgcgatta ttatttgtag tgtgtcagcg atttatggtt tgggtgatcc
     2041 tgatgcctat ttaaaaatgt tattgcatgt gagtcgtggc gaacgaatcg accaacgagc
     2101 tattttgcgt cgtttggccg aaatgcaata tcaacgtaat gatttagagt ttagtcgagg
     2161 gacttatcgg gtgaggggtg aggtgattga tgtttttcct gccgagtctg atgatgaagc
     2221 aataagaatc agtttgtttg atgatgaagt agagggaatt cattattttg acccgttaac
     2281 gggcgcacca ttgcgtaaag cagcacgagt cactatttat ccaaaaacac actacgtcac
     2341 ccccaaagag caaatcgaac aagcacttgg cactattcgc gccgaattag aaccgcgttt
     2401 gcagtggttt atgggcgaaa acaaactgat tgaagcacag cgtttaaaag agcgtaccca
     2461 atatgattta gaaatgttgc aacaactcgg ttattgcaat ggtatcgaaa attattcgcg
     2521 gcatttgtct ggacgaaaag agggcgaagc tccgccaacc ttgtttgatt atgtgcctgc
     2581 cgatgcgctg ttggtgattg atgaatctca tgtcaccgta ccacaaattg gtgcaatgta
     2641 taaaggcgat agagcgcgta aagaaaactt agtgaattat ggttttcgat tgccgtcggc
     2701 cttagataat cgaccaatga agtttgagga atgggaaaaa attagcccac aaaccatttt
     2761 tgtctcggca acccctgcca attatgagca agaacattgc ccgattgcca ttgagcaagt
     2821 ggttcggcct acgggtttgg ttgatcctga cattgaaata cgtcctgctt ttacccaagt
     2881 tgatgattta atgtcagaaa ttaatgcgcg tattttg
//
LOCUS       DAOQMB010000288         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19584_length_2907_cov_7.827489, whole genome shotgun sequence.
ACCESSION   DAOQMB010000288 DAOQMB010000000
VERSION     DAOQMB010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19584_length_2907_cov_7.827489"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..733)
                     /locus_tag="PLY05_08910"
     CDS             complement(107..733)
                     /locus_tag="PLY05_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HNC03458.1"
                     /translation="MRTLALCLAVSSLAFAPVAAMAESPWTVKVGFSQVNPKSDNGRL
                     NNALDSEVSSEVGFTPAIEYKFAPNIVGEVLLAIPFEHEVKVTGGVLNDAKVATFKHL
                     PPTFSAKYLFTPDAAFSPYVGLGLNYTLVFDEKTTGALLGTKLKGKDSFGVAANVGFE
                     YRMPNSPWGITADVRYIKIKSDLTLNGADIGTLTVDPMVLGVSAAYHF"
     gene            complement(1016..2140)
                     /locus_tag="PLY05_08915"
                     /pseudo
     CDS             complement(1016..2140)
                     /locus_tag="PLY05_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005222287.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            complement(2293..>2907)
                     /locus_tag="PLY05_08920"
     CDS             complement(2293..>2907)
                     /locus_tag="PLY05_08920"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393102.1"
                     /note="Catalyzes the only substrate-level phosphorylation
                     in the TCA cycle; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HNC03459.1"
                     /translation="GIKLVVCITEGVPTIDMLYVKEYIVRKGGVRMIGPNCPGIITPG
                     QSKIGIMPGHIHLPGKVGIVSRSGTLTYEAVKQTTDLGFGQSTCIGIGGDPIPGMTFI
                     DALALFQNDPQTEAIIMVGEIGGSAEEEAAAFIKANVTKPVVSYIAGVTAPAGKRMGH
                     AGAIISGGKGTAAEKFAALEAAGVKTVANPAELGKAIAEVTGWK"
BASE COUNT          889 a          625 c          501 g          892 t
ORIGIN      
        1 actctatcaa ttagctagtg cttattacca aaaagatttt gccagacatt tataaaaaat
       61 cgctcaaaga aatgaggtag gcacaaggcc tacccctaca caaattttaa aagtgatatg
      121 ctgcactcac acctaaaacc attggatcaa cagttaatgt accaatatct gcaccattta
      181 acgttaagtc acttttaatt ttgatataac gtacatcggc ggtaataccc caaggactat
      241 ttggcatacg gtattcaaaa cccacatttg ccgccacacc aaaactgtct ttacctttta
      301 actttgtacc aagcaatgca cctgtagttt tttcatcaaa aactaaggtg taatttagcc
      361 ccaaaccgac ataaggtgaa aaagccgcat cgggagtaaa taaatatttt gcactaaacg
      421 taggtggtaa atgcttaaat gttgctacct tagcatcatt taaaacacca ccagtaactt
      481 taacctcatg ctcaaaagga atcgctaaca gtacctcacc aacaatattg ggagcaaatt
      541 tgtattcaat agcaggggta aaaccaactt ctgaacttac ttctgaatcc aaagcattat
      601 tcaaacgacc attatcactt ttaggattaa cctgactaaa accaactttt acagtccaag
      661 gggattcagc cattgctgcc actggagcaa aagctaaaga actcactgcc aaacacaatg
      721 ctaaagtacg cataagcgtt ctccttacta atgggactaa atatttcaaa atgtttctta
      781 ctttttgaga atatgcctat ttagccataa tgtccatatt ttttacatgg ttttattgca
      841 aaaattgacc atataatata tatggtcaat ttgcttaata agatttgggt ttagcacctg
      901 ctgcggcttg ggcttgtagc tctttttcgc gtttccattg attaatcttt tgttgctcaa
      961 gttcttcttt gcgtttagcg tccatttctt cggggctaga acgatcaaaa cttaatgatg
     1021 cacttgaaaa agtattaggt tcgccacgtt tactttttaa tttaatattt aaacgcaatt
     1081 cattggcaga atcggcatta cgaatggctt cttcatacga aatatgacca tcgttatata
     1141 gctcaaataa tgcccagtca aaggtacgca tccccaattc acgcgatttt tccataatcg
     1201 gtttaatgcc atgaaactca gatttaaaaa tcaattctga aatcatttgc gtatttaata
     1261 gaatttcgat agccgctttt cgccctttac catcttgggt acgtaccaaa cgttgtgaaa
     1321 taatcgctcg taagttagct gataaatcca tcaacaattg gttacggcgt tcatcaggga
     1381 aaaagttgat aatacgatct aaggtttggt tggtactatt agcgtgtaat gttcccaaac
     1441 acaaatgtcc tgtttcggca aaagcaatcg catgctccat ggtttctgca tcacgaattt
     1501 caccaatcaa aataacatct ggagcttggc gtaaggtatt ttttagcgcg tgatgccaac
     1561 tgtgagtatc tacgcctacc tcacgatggg taattaatga catcttcgat tgatgaacat
     1621 attcaactgg atcctctacc gtaataatgt gtcctttaga ggtacgatta cgatgatcaa
     1681 tcatggctgc caaagacgtt gatttaccag aacctgtgcc tcctaccacc aaaacaagtc
     1741 cgcgcttatt cataatcact tctttgagta cttctggtag cgctaatttt tcaaaattag
     1801 gaatttctgc cgcaatggta cgaatcacca tgccgacaca ttgttgctga acaaaaacat
     1861 tcacccgaaa acgtgaaaca ttaggaacat taatcgcaaa attacactcc atttctttgg
     1921 caaattcagc acgttgggta tcattcataa tactttgtgc taatttttcg gtaatttctg
     1981 gagtaagctt ttgattagat aatggctgca tatcaccatt aattttcata ctaggcggaa
     2041 aatcattggc aataaataaa tctgagccgc ctgcttgatt catggcagtc agcaatttca
     2101 tcatataggc gcgagcttgt tcttcggtaa aggttgacat aattttatag ttttcaataa
     2161 attttaagat ggctaacttt agcaaaacat agttaaatcg tttttggttt tttgtgaact
     2221 atttcacgct ttaaaaacaa aaagccatcg tggtttcacg atggcttttt tattgcgaag
     2281 tgctaactcg gtttatttcc aaccagtcac ttcggcaatg gctttaccta attcggctgg
     2341 gttagcaacg gttttcaccc ctgctgcttc taaagctgca aatttttcgg cagccgttcc
     2401 tttaccaccc gagataatcg cgcctgcatg tcccatacgt ttacccgcag gcgctgtgac
     2461 acctgcaata taagacacaa caggtttagt cacgttggct ttaataaatg ctgctgcttc
     2521 ttcttcggca gaaccaccaa tttcaccgac cataataatc gcttcggttt gtggatcatt
     2581 ttggaataag gctaaagcat caataaacgt cataccggga atggggtcac caccaatacc
     2641 aatacaggta ctttgaccaa aacctaaatc ggtagtttgt ttaaccgctt cataagttaa
     2701 tgtacccgag cgagaaacaa tcccaacttt acctggtaag tgaatatgac cgggcataat
     2761 accaattttg gattgaccgg gtgtaataat acccggacag ttaggaccaa tcatacgcac
     2821 accgccctta cggacgatat attcttttac gtataacata tcaatggttg gtacaccttc
     2881 ggtaatacaa accactaatt taatgcc
//
LOCUS       DAOQMB010000289         2903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19633_length_2903_cov_4.074789, whole genome shotgun sequence.
ACCESSION   DAOQMB010000289 DAOQMB010000000
VERSION     DAOQMB010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19633_length_2903_cov_4.074789"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PLY05_08925"
     CDS             <1..699
                     /locus_tag="PLY05_08925"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HNC03460.1"
                     /translation="EQTFQIEESELVAEPLPTTDNRQAILFYEAAEGGAGVLTRLAQE
                     PNSLALVASTALQIMHFNKPEQGEWTIEGLAKQELRKADGSRICEAGCYQCLLSYFNQ
                     PDHDYIDRRQKDTLALLVSLANAHVTSKVTMPVELSSNSDSLANQWLNALKSYALRHP
                     DAFNVPVNQGAATAFAQYKSARLLVFIEPISTDVSVSVVNKGWQVLDFSQVSQWPTLF
                     EQYASLIGKIENLQ"
     gene            696..>1230
                     /locus_tag="PLY05_08930"
     CDS             696..>1230
                     /locus_tag="PLY05_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03461.1"
                     /translation="MTALNIEFVAGNLVRARGREWVVQPDSQGQLLRLRPLGGADDDT
                     IAIVPELEFEPVVPAVFPWPTTGQIGNHAAALLLKDALRLKLRSGAGPFRSFGNIAVE
                     PRAYQLVPLLMALRLSTVRLLIADDVGIGKTIEAGLIVRELMDRGEVARLAVLCPPHL
                     VEQWQQELATHFNLQAVA"
     assembly_gap    1231..1240
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1241..>2903
                     /locus_tag="PLY05_08935"
     CDS             <1241..>2903
                     /locus_tag="PLY05_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC03462.1"
                     /translation="NSASRIERDVPHGVSLFDHYHCVVVSLDYIKSERHREHFLSIAP
                     ECIIVDEAHTCATNGQGKQLRFQLLQRLVADANRHLIMLTATPHSGDEVAFYNLLSLL
                     NPAFSVLQTRTSAADPLRQELARHFVQRRRKDIDEWQDTRGFPKRMKTEITYQLKGAW
                     GEFFDDVQAYCRELAENIEQTANGNARLIWYATLALLRCVASSPAAAEKALTTRLDGK
                     LEEDEYLADSRLYDGGDDDLQSNDQEPSACLQEAGRLEELIATATRLKGKSGDPKLVA
                     LIQHVDDLIKEGFHPVIFCRYVATAHYVADHLKKHFSSKVVTIDAVTGEYTSEERKQR
                     VEALAEEPARILVATDCLSEGVNLQHVFTAVIHYDLAWNPTRHEQREGRVDRFGQPAE
                     QVRCTMLYGQDNPVDGFVLHVILRKADAIRQELGVLVPMPEDAQRINQALVKAALMKR
                     DRSPKFQQAVLDFGDELENDLKPLQAQWHDALEKAKANRTVFAQRRIKPEEVLPEWQK
                     QQTALGSEADVQRFVQNACARLNAPLEPSCGNSFRLLPQHLPQVLR"
BASE COUNT          800 a          581 c          696 g          816 t
ORIGIN      
        1 gagcaaacct ttcaaataga agagtctgag ttagtcgcag agccattacc gacaacagac
       61 aacagacaag cgattttgtt ttatgaagcg gcggaaggtg gtgcaggcgt attaacacga
      121 ttagctcaag agccaaatag tctggcatta gtggcaagta ccgctttgca aattatgcac
      181 tttaataagc cagagcaagg ggaatggacg atagagggtt tggcaaagca agaattgcgt
      241 aaagccgatg gttcacgaat atgtgaagca ggttgttatc aatgcttatt gtcgtacttt
      301 aatcagcctg accatgacta tattgaccgc cgccagaaag acaccttggc gttattagtg
      361 tcacttgcta atgctcacgt gacatctaaa gtgacaatgc ctgttgagct atcatcaaat
      421 agcgactcac tggctaatca atggcttaat gctttaaaat cttatgcctt gcgtcaccca
      481 gatgcgttta atgtgcccgt gaatcagggt gcagcaaccg cttttgctca atacaaaagt
      541 gcacgattat tagtgtttat tgaacctatt agcaccgatg tcagcgtgag tgttgtcaat
      601 aaaggttggc aagttttaga cttttcacag gtttcacaat ggccaactct ttttgagcag
      661 tatgccagtc ttattggcaa aatcgagaat ctacaatgac ggcattaaat atagagtttg
      721 tggcaggtaa tttagtccgc gctcgtggcc gtgagtgggt ggtacaacct gactcgcaag
      781 gtcaactatt gcgtttgcgt cccttaggtg gtgcagatga cgataccatt gctattgtac
      841 ctgaattaga gtttgagcct gttgtacctg ccgtatttcc gtggccaaca actgggcaga
      901 taggcaatca tgccgctgct ttattattaa aagatgcttt gcgcctgaag ttacgttcgg
      961 gtgcagggcc ttttcgttct tttggtaata tcgcggtaga gccaagggct tatcaattag
     1021 tcccattgtt gatggccttg cgtttaagca cggttagatt actaattgca gatgatgttg
     1081 ggattggtaa aaccatcgag gctggtttaa ttgttcgtga attaatggac agaggtgagg
     1141 ttgcgcgtct agcggtattg tgtccgccac atttagttga acaatggcag caagagttag
     1201 caacgcattt taacttacag gcggttgcct nnnnnnnnnn aatagtgcca gtcgtattga
     1261 gcgtgatgtg cctcatggtg ttagtttatt tgaccattat cattgtgtcg tggttagcct
     1321 tgactacatt aaaagcgaac gccaccgcga gcatttttta tcaattgccc ccgaatgtat
     1381 tattgttgat gaggcacata cttgcgccac gaatgggcaa ggcaagcaat tacgttttca
     1441 actattgcag cgtttagtgg cagatgctaa ccgtcattta attatgctaa cagcaacgcc
     1501 tcattcgggt gatgaagtcg ctttttataa tttattatcc ttattaaatc ctgcgtttag
     1561 tgtcttgcaa acacgcacct cggcggcaga ccccttacgc caagagcttg ctcgtcattt
     1621 tgttcagcgt cgtcgtaaag atattgacga gtggcaagat acacgcggct ttcccaaacg
     1681 catgaaaaca gaaattacct atcagttaaa aggagcatgg ggtgagtttt ttgatgatgt
     1741 tcaagcctat tgtcgagagt tggcggaaaa cattgagcaa accgctaacg gtaatgctcg
     1801 cctgatttgg tatgcgacac tcgcattatt acggtgtgtc gcttcatcac cagccgccgc
     1861 cgaaaaagca ttaacaacac gcttagacgg taaattagaa gaagacgaat atttagcgga
     1921 tagccgtctg tatgatggcg gtgatgatga tttgcaaagt aatgaccaag aaccgtctgc
     1981 ctgtttacaa gaagcagggc gtttagaaga gttgatagct accgctaccc gtttgaaggg
     2041 caaatcaggt gatccgaagt tagttgcttt aattcaacat gttgatgact tgattaaaga
     2101 aggttttcat ccagttattt tttgtcgata tgtggcaaca gcgcattatg tcgctgacca
     2161 tcttaaaaaa catttttcat ccaaagtagt gaccatagat gcggtaacgg gtgaatatac
     2221 ctccgaagaa cgtaagcaac gcgtggaggc attagccgaa gaacctgcgc gtattttggt
     2281 agctaccgat tgtttgtcag agggggtgaa tttacaacat gtatttacgg cggttattca
     2341 ttacgattta gcgtggaatc caacacgcca cgagcaacgg gaaggtcgtg tcgatcgttt
     2401 tggtcaacca gcagaacagg tacgttgtac tatgctttat ggacaagata accctgtaga
     2461 tggttttgtc ttacatgtta ttttacgcaa agcggatgct attcgccaag agctaggcgt
     2521 attagtgcca atgcccgaag atgctcaacg catcaatcag gcattagtta aggctgcatt
     2581 aatgaaacgt gaccgaagcc ccaagtttca acaagcagta ttagactttg gcgatgaatt
     2641 agaaaatgat ctaaagcctt tgcaagcaca atggcatgat gctcttgaaa aagccaaagc
     2701 taaccgcacg gtttttgccc aacgacgtat aaagcccgaa gaagttttac ctgaatggca
     2761 aaaacaacaa acagctttag ggagtgaggc agatgtacaa cgcttcgttc aaaatgcctg
     2821 tgcgcgttta aatgcacctt tagagccaag ttgtggtaat agttttagat tgctacctca
     2881 gcatttgcca caagtgttac gct
//
LOCUS       DAOQMB010000290         2894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19728_length_2894_cov_16.447693, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000290 DAOQMB010000000
VERSION     DAOQMB010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19728_length_2894_cov_16.447693"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="PLY05_08940"
     CDS             complement(<1..1160)
                     /locus_tag="PLY05_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005217605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03463.1"
                     /translation="MSQEQQRRWRLLLGNMPQLTPELNARDLGMDAALAALYNQGDEG
                     EDQGFASSQKSKQRQAGLGGSAPKVSRWLGDIRQYFPSSVVRVLQKDAYQRLNLEQML
                     LEPEMLQAVEPDVHLVSTLISLNHLMPAKTKETARLVVRKVVDELERRLAGKMQQAVT
                     GSLNRANRTRRPKRAQDIDWARTIRANLQHYLPEHNTIIPQNLVGYGRHRQSVKDIIL
                     CIDQSGSMASSVVYASLFGAVLASIKAVSTQLVVFDTAVVDLTPMLDDPVDVLFGTQL
                     GGGTDINKALAYCQGLIRRPEDTILVLISDLYEGGNNTELLKRTASLIGSGVQMISLL
                     ALADDGAPCFDARNAAQFTALGSPTFACTPDLFPDLMAAALNKQDVGVWAAQ"
     assembly_gap    1161..1260
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1261..>2894)
                     /locus_tag="PLY05_08945"
     CDS             complement(<1261..>2894)
                     /locus_tag="PLY05_08945"
                     /inference="COORDINATES: protein motif:HMM:NF040143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HNC03464.1"
                     /translation="PIGLLAQAAGYYDSERFWEHLVEQRPHVGEVFQAIHEAMSAIRE
                     QVIDDGSPRQQREACREAYMRQCLRQAEKDGYQQIVVVCGAWHTPALVELKTTKKTDT
                     ALLKTLNKIKTQAAWIPWTYSRLLTSSGYGAGIESPAWYQHLWTYYQHDPIDAEKISI
                     SWLAKFAQELRAQGLDASSAQLIDAARLIASLAALRGRELPDLDDLNEAIVSILHHGN
                     ALPSQLAHKMLVGEVLGSVPDDVPSLPIQQDFLKLCKSLRLKQEADSKALELDLRKDL
                     DLARSQFLHRLNLLGITWGNYQGGGGRGSFKENWILQWQPELSLKLLETAVWGQSIQE
                     ATDNYIQHQLIHEQHIAKVAQLMQSVLLADLAQTMPALIRHIMNLSAQSSDISHLIDA
                     LEPLANTWRYSDVRKTDSQAVGDVVESFVTRICIGLLPACNSLNDDAAEQRLTELKTI
                     DRVLQRLEKPSLTQAWHQTVLKLTTMANLHGLIAGWCCRIAQNYELLSAEQAAERFAL
                     ALSQANNPEQAAHWFAGFLTDQGLSLLHDEALWQLV"
BASE COUNT          756 a          669 c          599 g          770 t
ORIGIN      
        1 ttttgagcag cccaaacgcc aacatcttgt ttattgagtg cagctgccat taaatcggga
       61 aataaatcgg gtgtacaggc aaaagtgggt gaacctaaag cggtaaattg agcggcatta
      121 cgcgcatcaa aacatggcgc accatcatcc gccaacgcca acaaactaat catttgcaca
      181 ccactaccaa tcaagctcgc cgtacgtttg agcagctctg tgttattgcc accttcgtat
      241 aaatcactaa ttaataccaa aatagtatct tcaggccgac gaatcaaacc ttggcaataa
      301 gcaagggctt tattaatgtc cgtaccaccg cccaattgtg taccaaacaa cacatcaaca
      361 gggtcatcta gcatgggcgt taaatcgacc accgctgtat caaacaccac taattgtgtg
      421 ctaacggctt taatggaggc taacaccgcc ccaaataaac tggcataaac caccgaactc
      481 gccattgagc cactttggtc gatgcacaaa ataatgtctt taaccgattg gcgatgtcga
      541 ccataaccca ctaaattttg cggaataatg gtgttatgtt cgggtaaata gtgctgtaaa
      601 ttagcacgaa tggttcttgc ccaatctata tcttgggcgc gttttggccg acgtgtgcga
      661 ttggctcgat tgaggctacc tgtcacggct tgttgcattt tacctgccaa acgccgctct
      721 aattcatcga ctaccttacg taccacgagt cgtgcagttt ctttggtttt agcaggcatt
      781 aaatgattca gactaatcag cgtactgact aaatgcacat caggttcaac tgcttgtagc
      841 atttctggct caagcaacat ttgttctagg tttagtcgct gataagcatc tttttgcaac
      901 actctgacca ctgatgaggg aaaatattgc ctaatatcac ccaaccaacg gctgactttg
      961 ggcgcagaac cccccaaacc tgcttggcgt tgttttgact tttggctgct ggcaaaccct
     1021 tgatcttcac cttcgtctcc ttggttatat aaagcggcga gggcagcatc catgcctaaa
     1081 tcacgggcat ttaattcggg ggttaattgc ggcatattgc ctaataataa acgccagcgg
     1141 cgttgttgtt cttggctcat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 tcaaccagtt gccataaggc ttcatcatgc aacaaactta aaccttggtc ggttaaaaag
     1321 cctgcaaacc aatgtgccgc ttgttctgga ttattggcct gagataaagc taaggcaaag
     1381 cgttcagccg cttgttctgc gcttaataac tcataatttt gggcaatacg acaacaccaa
     1441 cctgcaatca aaccatgcaa attagccata gtagttagtt taagcacggt ctgatgccat
     1501 gcttgagtca aagagggttt ttctaaacgc tgtaacacgc ggtcaatggt ttttagttcg
     1561 gttaaacgtt gttcggcggc atcatcattt aagctattac aggcagggag caagccaata
     1621 caaatacggg tgacaaaact ttccaccaca tcacccaccg cttggctatc ggttttgcgc
     1681 acatcactat aacgccatgt attagctaat ggctcaaggg catcaattag gtggctaata
     1741 tcgctgctct gtgccgataa attcataata tggcgaatta gcgcaggcat ggtttgggct
     1801 aaatcggcca ataacaccga ctgcattaat tgtgcaactt tagcaatatg ttgctcgtga
     1861 atcagttggt gttgaatata attatccgtc gcctcttgaa tgctttgtcc ccaaaccgcc
     1921 gtttctaaaa gttttaggct gagttcgggt tgccattgta aaatccaatt ttctttaaaa
     1981 ctgccacgac cgccaccgcc ttgataattg ccccatgtaa tgcctaataa attcaagcga
     2041 tgcaaaaatt ggctacgggc aaggtctaag tctttacgta aatctagttc taaggctttg
     2101 ctgtcggcct cttgttttaa gcgcagactt ttacacagtt ttaaaaagtc ttgttgaatc
     2161 ggcaggctag gcacatcatc aggcacagag cccaacacct cacccaccaa cattttatgg
     2221 gctaattggc tgggtaaagc attgccatga tgcaaaatgc tgacaatggc ttcgtttaaa
     2281 tcatctaaat cgggtagctc tcgcccccgt aacgctgcca aagaagcaat taaacgtgcg
     2341 gcatcaatca attgtgcact agaagcatct agaccttgag cgcgtagctc ttgagcaaat
     2401 ttggccaacc aactgatact gattttttcg gcatcaattg ggtcgtgttg ataataagtc
     2461 cataagtgtt gataccaagc aggcgactcg atgcccgcac cataaccact gctggttaat
     2521 aaacggctgt aagtccacgg aatccatgcg gcttgagttt tgattttatt gagggttttt
     2581 aacagggctg tatcggtttt tttagtggtt tttagctcaa caagggcagg ggtatgccat
     2641 gctccacaca ccacgacgat ttgttgataa ccgtcttttt cggcttgacg caaacattga
     2701 cgcatatagg cttcgcggca ggcttcacgt tgttggcgcg ggctgccatc gtcaatcact
     2761 tgctcacgaa tcgccgacat ggcttcatgt atggcttgaa agacctcacc aacatgaggg
     2821 cgttgttcga ctaaatgctc ccaaaaacgt tcactgtcat aatagcctgc ggcttgtgcc
     2881 aataaaccaa tggg
//
LOCUS       DAOQMB010000291         2880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_19903_length_2880_cov_15.182655, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000291 DAOQMB010000000
VERSION     DAOQMB010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19903_length_2880_cov_15.182655"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLY05_08950"
     CDS             <1..465
                     /locus_tag="PLY05_08950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770766.1"
                     /note="NuoCD; NDH-1 shuttles electrons from NADH, via FMN
                     and iron-sulfur (Fe-S) centers, to quinones in the
                     respiratory chain; subunits NuoCD, E, F, and G constitute
                     the peripheral sector of the complex; in Escherichia coli
                     this gene encodes a fusion protein of NuoC and NuoD that
                     are found separate in other organisms; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HNC03465.1"
                     /translation="GYENFDFEVPIAHNGDAYDRCLVRIEEMRQSIRIIKQCLENMPA
                     GDYKADHPLTCPPPKEKTLQHIETLITHFLSVSWGPVMPAGEACQLIEATKGINSYYL
                     TSDNATMSYRTRIRTPSFAHLQQIPSVINGSMIPDLIAYLASIDFVMADVDR"
     gene            564..1073
                     /gene="nuoE"
                     /locus_tag="PLY05_08955"
     CDS             564..1073
                     /gene="nuoE"
                     /locus_tag="PLY05_08955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004794328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HNC03466.1"
                     /translation="MIICTSSRPKFTDGYVMSEQERHDIEHALEHYEDSRAASIDALK
                     IVQKKHGWVPDSAIYAIADIIKIPASDVDGVATFYNKIFRKPVGRNVIMVCDSIGCFL
                     TGYEDLIAEIKQQLNIDYGQTTPDNRFTLLPICCLGNCDKGPTLMINEDTHGPVSIEQ
                     VSSLLEQYP"
     gene            1070..2365
                     /gene="nuoF"
                     /locus_tag="PLY05_08960"
     CDS             1070..2365
                     /gene="nuoF"
                     /locus_tag="PLY05_08960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008941874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HNC03467.1"
                     /translation="MTVVSADTRPLTWRLQHGAEITDIKTYESLEGYAAVRKAIKDMS
                     PQDVQKLVKDANLRGRGGAGFPTGVKWSLIPMGEDAGVKYLVCNADEMEPGTFKDRLL
                     MEKLPHQLVEAMIVAGYAIQATRAYIFLRGEYILAADRLNAAIEQAKAAGYLGNNILG
                     SGWSMDLYVHTGAGRYICGEETALINSLEGRRANPRAKPPFPQIAGAWGKPTIVNNVE
                     TLCNIAPIIMRGAEWFKGLSEGKSPDAGTKLYGVSGRAKRTGVWELPIGTTARELLEV
                     HAGGMKDGLNLKCWLPGGASTDFLMPEHLDLAMDYDTIMKAGSRMGTGLIMVVDDKQN
                     MISVVRNLEEFFARESCGWCTPCRDGLPWGVKILRALERGEGHPDDIEQLSKLTRDLW
                     IGKTFCAHAPGAMEPLMSALKYFRSEFEQGIAKDAMING"
     gene            2372..>2880
                     /locus_tag="PLY05_08965"
     CDS             2372..>2880
                     /locus_tag="PLY05_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNC03468.1"
                     /translation="MATIHVDGKQYDVDGADNLLQACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYANAEDKRGRLVMSCMTPSTDNTFISIDDAEAKEFRASVVEWLMTNHPHD
                     CPVCEEGGHCHLQDMTVMTGHDRRRYRFTKRTHQNQDLGPFINHEMNRCIACYRCVRY
                     YKDYAGGED"
BASE COUNT          782 a          594 c          698 g          806 t
ORIGIN      
        1 ggttatgaaa actttgactt tgaagtgcca attgcccata acggtgatgc ctacgaccgt
       61 tgtttggtgc gtatcgaaga aatgcgtcaa agtattcgta ttatcaagca gtgtttagaa
      121 aatatgcctg cgggtgatta taaggctgac catccgttaa cctgtccacc acccaaagaa
      181 aaaaccctac aacacattga aaccttgatt acccacttct tgagtgtatc gtgggggccc
      241 gtcatgcccg caggtgaagc ctgccaattg attgaagcca ctaaaggtat caatagttat
      301 tatttgacca gtgataacgc caccatgagt tatcgtacgc ggattcgtac accaagtttt
      361 gcccatttac agcaaattcc atcggtcatt aatggcagca tgattccaga cttgattgct
      421 tatttagcca gtatcgactt tgtaatggct gacgttgacc gttaagagct gaacttgcgt
      481 atcgcacccc cctcagtccc cccttgttaa ggggggatgc caaaggcagg ggggtatgta
      541 agttcttaga gaggcttgaa aaaatgatta tttgtactag tagccgtccc aaatttactg
      601 acggttatgt gatgtctgaa caagagcgtc atgatattga acatgcgctt gagcattatg
      661 aagattctcg tgcggcgtct attgatgcgc taaaaattgt gcaaaagaag catggttggg
      721 tacctgacag tgccatttat gccattgccg atattattaa aatccctgct tccgatgtcg
      781 atggcgttgc cactttttat aacaagattt tccgtaagcc tgttggccgt aacgtgatta
      841 tggtttgcga cagtatcggt tgttttttaa caggttatga agatttaatt gctgaaatta
      901 aacagcaatt aaacatcgac tatggccaaa ccacgccaga taaccgtttt accttattac
      961 ccatttgctg tttaggtaac tgtgacaaag ggccaacgtt aatgattaac gaagacaccc
     1021 acggaccagt cagcattgaa caagtttctt ctttattgga gcagtatcca tgactgtggt
     1081 atctgccgac actcggccat taacatggcg actccaacat ggcgcagaaa ttaccgatat
     1141 taagacctat gagtctttag aaggttatgc agcagtacgc aaagccatta aggatatgtc
     1201 gccacaagac gtacaaaaac tggtcaaaga tgccaattta cgcggacggg gcggtgcagg
     1261 cttccccaca ggcgtgaagt ggagcttgat tccgatgggc gaagacgcag gggtaaaata
     1321 cctcgtctgt aacgccgatg aaatggaacc gggtacattc aaagaccgct tattgatgga
     1381 aaaattaccc catcaattag ttgaggcgat gattgttgcg ggttatgcca ttcaagccac
     1441 acgtgcctat atcttcttgc gtggcgaata cattttagca gcagaccgct taaatgctgc
     1501 gattgaacaa gccaaagccg caggttattt aggtaacaat attttaggct ctggctggag
     1561 tatggattta tatgttcata caggcgcagg ccgttatatt tgtggtgaag aaaccgcatt
     1621 aattaactct ttagaaggcc gtcgtgctaa tccacgcgcc aaaccaccgt tcccacaaat
     1681 tgctggggca tggggtaaac caacgattgt taacaacgtt gagaccttat gtaacatcgc
     1741 gccaatcatt atgcgtggtg ctgaatggtt taaaggtttg tctgaaggca aaagtcccga
     1801 tgcaggcact aaattatatg gtgtctctgg tcgtgctaaa cgtacaggcg tttgggagtt
     1861 acccattggc acaaccgcac gtgaattgtt agaagttcat gcaggtggta tgaaagatgg
     1921 tctgaattta aaatgttggt tacctggtgg ggcatcaact gactttttaa tgcctgaaca
     1981 tctcgattta gccatggatt acgacaccat tatgaaagca ggcagccgta tggggactgg
     2041 cttgattatg gtggttgatg acaaacaaaa catgatctct gtggtgcgta accttgaaga
     2101 attctttgca cgagaatctt gtggttggtg tacaccgtgt cgtgacggtt tgccgtgggg
     2161 tgtgaaaatc ttacgtgctt tagaacgtgg tgaaggtcat cctgatgata ttgagcagtt
     2221 aagcaaactg actcgtgatt tgtggattgg taaaaccttc tgtgctcacg caccaggtgc
     2281 gatggagcca ttaatgagtg cgctcaagta tttccgctct gaatttgaac aaggcatcgc
     2341 caaagatgcc atgataaatg ggtaaacagg catggcaacg attcatgtag atggcaaaca
     2401 gtatgatgtt gatggtgctg ataacctgct tcaagcctgt ttgtctttag gtttagacat
     2461 tccttatttt tgttggcatc ctgccttggg cagtgtgggt gcttgccgtc aatgtgcggt
     2521 taagcaatat gctaacgccg aagataaacg tggtcgtttg gtgatgtctt gtatgacacc
     2581 ttccaccgac aacaccttta tttcgattga tgatgccgaa gctaaagagt ttagagccag
     2641 tgtcgtggaa tggttaatga ccaaccaccc acatgactgt ccagtttgtg aagaaggtgg
     2701 tcactgtcat ttacaagata tgaccgtgat gacaggtcat gaccgtcgcc gttatcgctt
     2761 cacgaaacgt acgcatcaaa accaagattt aggcccgttc attaatcatg aaatgaaccg
     2821 ttgtatcgcc tgttatcgtt gtgtgcgtta ttacaaagac tacgcaggtg gcgaagactt
//
LOCUS       DAOQMB010000292         2870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20017_length_2870_cov_15.843694, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000292 DAOQMB010000000
VERSION     DAOQMB010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20017_length_2870_cov_15.843694"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /gene="rlmH"
                     /locus_tag="PLY05_08970"
     CDS             complement(<1..371)
                     /gene="rlmH"
                     /locus_tag="PLY05_08970"
                     /inference="COORDINATES: protein motif:HMM:NF000986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HNC03469.1"
                     /translation="MRIRLLAVGQKMPDWVTTAYLDYAKRLNDDVRLELIEIPAAKRS
                     KNSEVDKMKQQEGEALLAAIHHNERVIALEVGGKEYSTHQLSQQMDNWLQGGQNVCLL
                     VGGPDGLSSDVIKRADGLWSLS"
     gene            complement(373..735)
                     /gene="rsfS"
                     /locus_tag="PLY05_08975"
     CDS             complement(373..735)
                     /gene="rsfS"
                     /locus_tag="PLY05_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HNC03470.1"
                     /translation="MKADNVSEQILTQALDALADIKAQNVLSIDVRNLTGIAEYMVIA
                     SGTSTRHVKALADNVVEKCKEAGFRPLGVEGERDAEWVLIDLAGVIVHVMLPTTRRFY
                     DLERLWMTPEAQQALPQT"
     gene            complement(725..1381)
                     /gene="nadD"
                     /locus_tag="PLY05_08980"
     CDS             complement(725..1381)
                     /gene="nadD"
                     /locus_tag="PLY05_08980"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HNC03471.1"
                     /translation="MSHLIAYFGGSFDPVHQGHLATAKFLVAYFQLNKLVFLPAYLSP
                     LKSQSLAAHHRVAMLKLAIQDETTFALDEQEVLQPSKSYTINILKNLRQVYGSTQPLA
                     FVMGMDSFLSLPKWHLWQELTDFSHLIVVNRPHYSAIFNTELQQWLNKRRCNQRQMLE
                     YQPAGLVYFVDSPFYAISSTDIRKALQQGHSTHQQLIPAVANYIYHHHLYGATATNES
                     "
     gene            complement(1405..2658)
                     /locus_tag="PLY05_08985"
     CDS             complement(1405..2658)
                     /locus_tag="PLY05_08985"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004854996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNC03472.1"
                     /translation="MSVQAYMQQLGQDARSASRVIARATTREKNAALQAIYQSLQANQ
                     EKVLAANAQDMAKGRASGLDAALLDRLELTPSRFAGMLEGLEQVIALTDPIGVITDLA
                     YRPSGIQVGKMRVPLGVIGIIYESRPNVTLEAAALCLKSGNATILRGGSEAINSNNAI
                     AECIQQGLVSANLPASVVQVVNTTDRAAVGQLITMPEYVDVIVPRGGKGLIERISNEA
                     RVPVIKHLDGNCHVFIEREADINKALTIAFNAKTHRYGVCNAMESLLIDAPIAAEILP
                     KLAELYASKNVELRGCERTRAIIDAQVASEQDWYEEYLAPVLAIKVVDNLDAAIEHIN
                     KYSSHHTEAIVTENYTKARRFLAEVDSSSVMVNASTRFADGFEYGLGAEIGISTDKIH
                     ARGPVGLEGLTSQKWIVLGDGHIRG"
BASE COUNT          872 a          602 c          584 g          812 t
ORIGIN      
        1 gataacgacc ataggccatc ggcacgttta atgacgtctg aacttaaacc atcagggcca
       61 ccaaccagta agcatacatt ttggccacct tgtagccaat tatccatttg ttgactaagt
      121 tgatgtgtgg agtattcttt gccgccaact tctaaggcaa tgactcgttc attatggtga
      181 atagcagcaa gtagggcttc accttcttgc tgcttcattt tatcgacttc gctattttta
      241 ctgcgtttgg ccgcaggaat ttcgattagc tctaaacgaa catcatcatt aagacgtttg
      301 gcatagtcca aataggcagt tgtgacccaa tctggcattt tttggccaac agcgagtaaa
      361 cgaatacgca tattaagttt gaggtaaggc ttgttgcgct tctggagtca tccacaaacg
      421 ctctaagtca taaaaacgac gtgtagtggg taacattacg tgaacaatca cgccagctaa
      481 atcaattaaa acccactcgg catcacgctc gccttcaaca cctaaagggc gaaagccagc
      541 ttctttgcat ttttctacca cgttgtcagc aagtgctttt acgtggcgag tagaagtacc
      601 actggcaatc accatatatt cggcaatacc agttaaatta cgaacatcaa tagacaaaac
      661 attttgtgct ttaatatcag caagtgcatc taaggcttga gttaaaattt gttcagaaac
      721 attatcagct ttcattcgtc gctgtcgctc cgtagaggtg atggtgataa atatagttgg
      781 caacggcagg aattaattgt tgatgggttg aatgcccctg ttgtaatgcc ttgcgaatat
      841 ccgtactgct aatggcataa aaagggctat caacaaaata aaccaagcct gctggttgat
      901 attctaacat ttgtcgttga ttgcagcgac gtttattcaa ccattgttgc aattctgtat
      961 taaaaatggc ggaatagtgg ggtctattga ccacaatcag atgggaaaaa tcagttaact
     1021 cttgccataa atgccattta ggcaaactta aaaagctgtc catgcccatg acaaaggcca
     1081 agggttgtgt tgagccataa acttggcgca agttttttaa gatgttaatg gtataagatt
     1141 ttgacggttg taaaacttct tgctcatcaa gagcaaaggt tgtttcgtct tgaatagcca
     1201 atttaagcat cgctacacgg tgatgtgcag ccaatgattg gctttttagt ggagataaat
     1261 aagctggcaa aaagaccaat ttatttaact gaaaataagc cactaaaaat tttgcggtgg
     1321 ccaaatgacc ctgatgtaca gggtcaaatg agccaccaaa gtaggcgatt aggtggctca
     1381 tattatttag gcgtaaataa acgattatcc acgaatatgg ccatccccta acacaatcca
     1441 tttttgagag gttaaacctt ctaaacccac aggaccacga gcatggattt tgtcggtaga
     1501 aatgccaatc tctgcaccta agccatattc aaagccatca gcaaaacggg tagaggcatt
     1561 aaccatcacc gagctagagt caacttcggc taagaagcga cgcgctttgg tgtagttttc
     1621 ggtcacaatc gcttcggtat ggtgtgagct atatttgttg atatgctcaa tcgccgcatc
     1681 taagttatca accactttaa tggctaaaac gggtgctaaa tactcttcat accaatcttg
     1741 ttcactagca acttgggcat caataatggc acgagtacgc tcacaaccac gtaattcaac
     1801 atttttactg gcatataatt cagcaagctt tggcaaaatt tctgctgcaa ttggcgcatc
     1861 aattaataag ctttccatgg cattacacac gccataacga tgtgttttgg cattaaaagc
     1921 aatggttaaa gctttgttaa tatcggcttc acgttcaata aaaacgtggc agttgccatc
     1981 taaatgttta atcacaggca cacgtgcttc attgctaata cgctcaatta agcctttgcc
     2041 gccacgaggt acaatcacgt ccacatattc gggcatggta attaattggc caactgctgc
     2101 gcgatcagtg gtattgacca cttgaaccac actagcaggt aaattggcag acactaaacc
     2161 ttgttgaata cattcagcaa ttgcattgtt ggagttaatg gcttctgaac caccgcgtaa
     2221 aatagttgcg ttacctgatt ttaagcacag cgcagcagct tctaaagtca cgttagggcg
     2281 ggactcataa ataatgccaa taacacccaa aggcacacgc attttgccca cttgaatgcc
     2341 tgacggacga taggctaaat cggtgattac accaatcgga tcagttaaag caatcacttg
     2401 ctctaagcct tctaacatac ctgcaaagcg actaggtgtt aattctaagc gatctaataa
     2461 tgcagcatct aagccactag cacggccttt ggccatatct tgggcattag cggctaacac
     2521 tttttcttgg ttagcttgta atgattgata aatggcctgt aatgcggcgt ttttttcacg
     2581 agtggttgcg cgtgcaatga cccgtgaagc agagcgggca tcttgcccta attgttgcat
     2641 ataagcttga acggacatgg taaatctcgg tcttgaagcc aaaaaaggtt ttgataaaaa
     2701 atacaaagat gaactagatt ataacagttg cgcttttaga gactagtgaa aatataacaa
     2761 tcatccgcca caatgtaaga atttggtgaa taacatagtt aaaaaagctt tttaatgatt
     2821 caaaaaattg caaagattat gttataaatt gccctcttaa cctaaacaac
//
LOCUS       DAOQMB010000293         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20207_length_2855_cov_4.944643, whole genome shotgun sequence.
ACCESSION   DAOQMB010000293 DAOQMB010000000
VERSION     DAOQMB010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20207_length_2855_cov_4.944643"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..1034)
                     /locus_tag="PLY05_08990"
     CDS             complement(372..1034)
                     /locus_tag="PLY05_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC03473.1"
                     /translation="MYIVIEGLKGVGKSTLLDALCLRLKQENVRFSLVTPTRPLPRFC
                     LVEFLAKHCRVLMRSDFFRCYLYRYRSNQHARQADWTQPLVLGDRSILTSLVTRWPKD
                     NQAQYIKKTKAQECEIPWPDEVIYLQMPLEPLLLRLKQRQRDYGKQDECVERLNLAFE
                     AYQELQRQGECLGVPSDLEWSVVDANQSPEQVLEAVYALLKLRCPKAWLRKQQMFHVE
                     SV"
     assembly_gap    1212..1311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1312..1527
                     /locus_tag="PLY05_08995"
     CDS             <1312..1527
                     /locus_tag="PLY05_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03474.1"
                     /translation="HRICDYPNKALVATMMCSAPTQQGHTRLLVSDDRQGIDPNVEQS
                     PLSSHELACLLGQFLCAYGSTMWPKAA"
     gene            1550..2098
                     /locus_tag="PLY05_09000"
     CDS             1550..2098
                     /locus_tag="PLY05_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HNC03475.1"
                     /translation="MLSSFQAQINNSVFVSEQGFIALQEQLNKTQARYLEVCEQRRIA
                     HELSGDGWHDNPEFNRQQQMEAFLNGEVKRLVQTLEKAKIFTVQEHARQIDKVWLGSV
                     VELEVTQCLTGNTHHEIWEIVGHGESNAELKKLAYDVPLARAMLTLQVDDCSDEFVLR
                     GEEVFISVVKLYASRQQAGLGE"
     gene            2098..2505
                     /locus_tag="PLY05_09005"
     CDS             2098..2505
                     /locus_tag="PLY05_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03476.1"
                     /translation="MMTTWAANELKTLAQQGYLVYLHTPYLQQQANRKNQAWQHKLMN
                     QPMSFLTQYDLLFRRLTQLLILQGYDFSNFHPHQTLKKLLLLLEGNLYSNAELSHVVE
                     CRHNLKYGFMDSPTPQAIKILDELSTRLQPFNA"
     gene            2532..>2855
                     /locus_tag="PLY05_09010"
     CDS             2532..>2855
                     /locus_tag="PLY05_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03477.1"
                     /translation="MANLPLITVVADVVGNLINGHYRHQQHMAELENEYRLEKARIEQ
                     ETIISCKKIDATLHYALANLAAQMEQSRQQFDLLVKNLDLQLEDNKKLEQHITELMKV
                     ATNPAE"
BASE COUNT          851 a          557 c          532 g          815 t
ORIGIN      
        1 taggccacta ctttgaggga gtattttttt aggttttctt ttattcataa tcgtaggata
       61 gtgaaccaaa cggaggttaa tagatcttag ctcattgtct cgactgtaca cagtattaat
      121 ggcaacaatt atgcgggtga caggtgatgt gtcaaaataa cccagctccc gttttggttc
      181 actttcttac aaataaccgt attgctgtgt tatatttttc catgaatcac aatttttgcc
      241 atttcaagaa tttatcgcat aatctttgtt atgttaaata gactcaaaat cccacaaaaa
      301 tagcttattt attaagcgtt ttgttttatt tgttacataa tacattattt gttatattta
      361 atacataggc tctaaacact ctccacgtgg aacatttgtt gttttcgtaa ccatgcttta
      421 ggacaacgca gctttagcaa agcataaacc gcctcaagta cttgctctgg cgactgattg
      481 gcatcaacaa ctgaccactc taaatcagat ggcacaccta aacactctcc ttgccgttgt
      541 aattcttggt aagcttcaaa cgccaaattt aaacgctcca cacactcatc ctgttttcca
      601 taatctcgtt gacgttgttt taatcgcaat aacaatggct ctaaaggcat ttgcaaataa
      661 atgacttcat caggccaagg aatttcgcat tcttgagctt tggtcttttt aatatactga
      721 gcttgattat cttttggcca acgggttact aaactggtca aaatactacg atcacccaat
      781 actaaaggtt gagtccagtc tgcttgacgt gcatgttgat tagaacggta acgatataaa
      841 taacaacgaa aaaaatcaga acgcatcaac acacggcaat gctttgctaa aaactcaact
      901 aagcaaaatc ttggcaatgg tcgtgtggga gtgactaaac taaaacgcac attttcttgt
      961 tttaggcgca aacacaaggc atccaacaaa gttgatttgc ccacgccttt taaaccctca
     1021 atgacaatat acatcaacgc caccccacta aaaatgcagt ggtgctatgg tggcctaagc
     1081 ggtcttaaca aagaaaaaac acaagcttgc agaaaagccg ttttgaggat ttggtttgca
     1141 taatagccac accgcgatga ttttggtgtg gtgaaataat gttagcactt acaaacaata
     1201 tggttagcaa tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncatcgtatt
     1321 tgtgattatc ccaacaaagc tttagtggcc acgatgatgt gctctgcccc cactcaacaa
     1381 ggtcacaccc gtttattggt cagtgacgac cgtcaaggca ttgatcctaa tgttgagcaa
     1441 agtcctttgt ctagtcacga actcgcttgt ttgttggggc agtttttatg tgcttatggc
     1501 tcaaccatgt ggcctaaagc cgcctaatta tttttttgga gtttttatca tgttgtcttc
     1561 ttttcaagcc caaatcaaca actcggtttt tgttagcgaa caaggtttta tcgctctaca
     1621 agaacaacta aacaaaactc aggctcgtta cttagaagtt tgcgagcaac gtcgaattgc
     1681 ccacgaatta agtggtgatg gctggcacga taacccagag tttaatcgtc agcaacaaat
     1741 ggaagccttt ttaaatggcg aggttaaacg cttagtgcaa acacttgaaa aagccaaaat
     1801 ttttaccgtg caagaacacg ctcgccagat agacaaagtg tggttgggtt ctgtcgttga
     1861 gctagaagta acccagtgtt taacaggcaa tacccaccac gaaatatggg aaattgttgg
     1921 tcatggtgaa tctaatgccg agctaaaaaa attggcttat gacgtgccat tggcgcgtgc
     1981 catgttaacg ctgcaagtcg atgattgctc agacgaattt gtgttacgag gtgaagaagt
     2041 ttttatcagt gtggttaagt tgtatgcctc acgtcaacaa gcaggcttag gagaataatg
     2101 atgacaacat gggctgccaa cgaacttaaa acattagctc aacaaggtta tttggtgtat
     2161 ttgcatacgc cctatttgca gcagcaagca aatcgtaaaa atcaggcatg gcaacacaag
     2221 cttatgaatc aacctatgag ctttttgacg cagtatgatt tgttattccg tcgtttaacg
     2281 cagttattaa ttttacaagg ttatgacttt tctaattttc acccacacca aaccttaaaa
     2341 aagctgttgt tgctgttgga aggtaatttg tatagcaatg cagagttaag tcatgtggtc
     2401 gaatgtcggc ataaccttaa atatggcttt atggatagtc caacaccaca agccataaaa
     2461 atattggatg agttaagcac tcgtcttcaa ccgtttaacg cataaattca cgatgaacag
     2521 aggtgcatat catggctaat ttacccctta ttaccgttgt ggctgatgtg gttggcaatt
     2581 taattaatgg ccattatcgt catcaacagc acatggccga acttgaaaat gaatatcgtt
     2641 tagaaaaggc tcgtatcgaa caagaaacca tcattagctg caaaaagata gatgcaaccc
     2701 ttcactatgc tttagcaaat ttggctgcac aaatggaaca atctcgccaa cagtttgacc
     2761 tattggtaaa aaacttagat ttgcaattag aggataataa aaaacttgag cagcatatta
     2821 ccgagttaat gaaagtcgcc acaaaccccg cagaa
//
LOCUS       DAOQMB010000294         2854 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20219_length_2854_cov_8.401929, whole genome shotgun sequence.
ACCESSION   DAOQMB010000294 DAOQMB010000000
VERSION     DAOQMB010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20219_length_2854_cov_8.401929"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLY05_09015"
     CDS             <1..705
                     /locus_tag="PLY05_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HNC03478.1"
                     /translation="PTRPRHYLYLIQEHLQGQSLRVWRKKNPHAPVETIIKFAKPIIK
                     ALRALHRRETLHQDIKPDNLFLTQSGCIKLIDFGSASVGSLAENLNLRAGAAEYAAPE
                     YALGIARDGRADQFSLAVTLYELLTGHYPYGENYKDCTTPKQFRKLSYTSACQYNPHI
                     PLWIDAALGQALSLNPEQRYADLSEFLHDLERPNPHLSLKSKPWIEQNPLVFWQVVSL
                     LLFLSNFLVLFLWLHG"
     gene            complement(682..1467)
                     /gene="mazG"
                     /locus_tag="PLY05_09020"
     CDS             complement(682..1467)
                     /gene="mazG"
                     /locus_tag="PLY05_09020"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNC03479.1"
                     /translation="MSYNIDDLRQIMVQLRSENGCNWDKQQSFESLVPYAIEEAHEVA
                     EAVINKNYQHLCEELGDLLLQVVFHAQIAQEQQLFSFDDVVNGISEKMIRRHPHVFAE
                     QHDLTPEQVSQQWATIKAQEKQNQLQDYLLDSVSLGRPALSVAEKLQKTVAKVGFDWP
                     DVRAVMLKIREELKEIEAAFQETPEKLEEEIGDLLFCVVNLMRHLKLDSEQTLKKANA
                     KFVRRFNFVEEQLKIRHKNLTDSDLAEMEHLWQLAKSTMQPQE"
     gene            1702..>2854
                     /locus_tag="PLY05_09025"
     CDS             1702..>2854
                     /locus_tag="PLY05_09025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC03480.1"
                     /translation="MSSYTKIFALSATLIAPFAQAATIIPAPPALENKAFVLMDYDSG
                     KILAESNAQEHLAPASLTKMMTSFLVEQRLLKGQLKEEDQVLMSQNAWCRGTSKESCM
                     YVPLNSYASVLDMLRGIIIQSGNDASKAVAEHLAGSETAFAELMNGEAKHLGMKNTQF
                     KNATGLTEDGHYTSAYDMALLAHAIIKDSSKYYPIYSEKEFTYNNIKQGNRNALLFTD
                     PTVDGLKTGHTDAAGYCLTASSKRGSMRLISVVMGANSMQARADQTRALFNWGFSNFE
                     SAQIRPKGQELAKPVVWFGQGESVSVGLANDMNVSLARGQKDRLQISIQVPSSLDAPI
                     KQGQEVGKIIATLDGKTVGEQALVALQPVEQAGFFTRVWHGIKRFFANLF"
BASE COUNT          844 a          594 c          528 g          888 t
ORIGIN      
        1 cctactcggc cacgccatta tttatattta attcaagaac atttacaagg tcaaagctta
       61 cgagtgtggc gcaaaaaaaa tcctcatgca cctgtagaaa cgattatcaa atttgccaaa
      121 ccgattatta aagctttacg tgctttacat agacgcgaaa ctttacacca agatattaaa
      181 cccgataatt tatttttaac ccaaagcggc tgtattaaat tgattgattt tggcagtgct
      241 agcgtgggga gtttggctga aaatcttaac ttacgtgcag gcgcggctga atatgccgca
      301 cccgaatatg ctttaggtat tgctcgtgat ggtcgtgccg atcaatttag tttagctgtg
      361 acattatacg aactattaac gggtcactat ccttatggag aaaactataa agactgcaca
      421 acacccaaac aatttcgcaa acttagctat accagtgctt gccaatataa tccgcatatt
      481 cctttgtgga ttgatgccgc acttggccaa gcgttaagcc taaacccaga acaacgttat
      541 gccgacctca gcgaatttct acacgattta gaacggccaa atcctcattt aagtcttaaa
      601 agcaaaccgt ggatagagca aaatccttta gtgttttggc aagtcgtttc tttattatta
      661 tttttaagca attttttggt gttattcttg tggttgcatg gttgatttag ccaactgcca
      721 caaatgctcc atttctgcca agtcactatc agtcagattt ttatgacgta tttttaattg
      781 ctcttcgaca aaattaaaac gccgcacaaa tttggcattg gcttttttaa gggtttgctc
      841 actatctaat tttaaatggc gcattaaatt gaccacacaa aacaacaaat caccaatttc
      901 ttcttcgagt ttttcgggtg tttcttgaaa tgcagcttct atttccttta attcttcacg
      961 tatttttaac attacggcac gcacatcagg ccaatcaaaa cctacttttg ccaccgtttt
     1021 ttgcagcttt tcggccacag acagcgcagg ccgccccaaa gacaccgaat ccaacaaata
     1081 atcttgcagc tgattttgtt tttcttgagc tttaatggtt gcccattgtt ggctcacttg
     1141 ttcgggcgtt aaatcatgtt gctcagcaaa aacatgcggg tgacgacgaa tcatcttttc
     1201 actaatacca ttaacaacat catcaaaact aaataattgc tgctcttggg caatttgtgc
     1261 atgaaacacc acttgcaata ataaatcacc caattcttca cataaatgtt gataattttt
     1321 attgatgacc gcttcggcaa cttcgtgggc ctcctcaatt gcataaggca ctaaggattc
     1381 aaagctttgt tgcttatccc aattacaacc attttcagag cgcagttgca ccataatttg
     1441 acgtaaatca tcaatgttat acgacataaa aattttctct ttattaggac ttacgcggtt
     1501 atcgcttatt tgttcacatt tcaggcattt tcaatacctt tctcatggag accgttatag
     1561 accaatatac tcacaaaacg cgctaatttt ggcaaaatgc gtaagtcttg tttattttgt
     1621 caagcaagtt atacaaacct ttttgcagaa taatgctaag atttgcgcca attaatttaa
     1681 cagattcata ggctttgtgt tatgtctagc tacaccaaaa tttttgcgct gagtgccact
     1741 ttaattgctc cttttgctca agccgccacc attattcctg caccacctgc tttagaaaat
     1801 aaagcttttg tgttaatgga ttatgattct ggcaaaattt tagccgagtc aaatgcccaa
     1861 gaacatttag cacccgcttc tttaaccaaa atgatgacca gttttttggt agaacaacgt
     1921 ttattaaaag gccaactcaa agaagaagac caagtattaa tgagccaaaa cgcatggtgt
     1981 cgtggtacaa gcaaagaatc ttgtatgtat gtgccgctta atagctatgc ctctgtgtta
     2041 gatatgctac gcggcattat tattcagtca ggtaacgatg cctctaaagc cgttgccgaa
     2101 catttggcag gcagcgaaac cgcttttgcc gaattaatga atggcgaagc caaacattta
     2161 ggcatgaaaa acactcaatt taaaaatgca acaggcttaa ccgaagatgg ccattatacc
     2221 tcagcttatg acatggcttt attggcacac gcgattatta aagatagcag caaatattac
     2281 cccatctact ccgaaaaaga atttacctac aacaacatca aacaaggcaa ccgtaatgcc
     2341 ttgttgttta ccgacccaac cgttgacggt ttaaaaacag gtcataccga tgccgcaggt
     2401 tattgtttaa ccgcttcgag taaacgtggt tcgatgcggt taatttcggt ggtgatgggt
     2461 gcgaatagta tgcaagcacg tgccgaccaa actcgtgctt tatttaactg gggttttagc
     2521 aattttgaaa gcgcacaaat tcgtcctaaa ggtcaagagt tagccaaacc agtggtgtgg
     2581 tttggtcaag gtgaaagtgt atcggttggt ttagctaatg atatgaatgt ttctttagca
     2641 cgtggtcaaa aagacagatt acaaattagt attcaagtac ctagttcttt agatgcaccg
     2701 attaaacaag gtcaagaagt gggtaaaatt attgccactt tggatggtaa aactgttggt
     2761 gaacaagctt tagttgcctt acaacctgtt gaacaagcgg gcttctttac tcgtgtttgg
     2821 cacggtatta aacgcttttt tgctaatttg tttt
//
LOCUS       DAOQMB010000295         2845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20348_length_2845_cov_3.325448, whole genome shotgun sequence.
ACCESSION   DAOQMB010000295 DAOQMB010000000
VERSION     DAOQMB010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20348_length_2845_cov_3.325448"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PLY05_09030"
     CDS             complement(<1..623)
                     /locus_tag="PLY05_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HNC03481.1"
                     /translation="MQLTTKGVVASFLIQHYIRLSGRIGFPFEQLLREANVQFTDLNP
                     SHGWVNQDILERLSQSTFLYYPDPLLGLRFLKMAEPAMLGVLGYMVQSSPTLLEMFHA
                     LNEFGHLVSTVSRPMLIHEPGVVLWTVRHQSDDLFFVRQCDETYIMACAILVKRQCPQ
                     ALKAIRFSHDPALENQHIHPIYEQLCRCPIYFNQPHSALVLDAQYLN"
     gene            1000..1920
                     /locus_tag="PLY05_09035"
     CDS             1000..1920
                     /locus_tag="PLY05_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005223425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HNC03482.1"
                     /translation="MKNIITATGIVLSLLAAAPTQAASTYSQTKYPVVLAHGMMGWDT
                     MLGIEYWYQIPNDLRDNGASVYTTQASGLESSEVRGEQLLKQVKDILAITGKGKVNLI
                     GHSHGGHSVRYVAAMIPNNIASVTTMATPAKGAPMADLIINGGSLGSVGTTIIQAAGT
                     MLNLLNGGSKLGLDVNANKSLASLSTKGSAEFNVKFPQAIPTTACGEGEYEVNGVKYF
                     SFAGTSPTTNALDLLDPAVKLVSLAFTNGEANDGFVGRCSAHVGKVVRDNYNMNHIDF
                     MNHIFGLRGLTTDPKAVYREQLNRLKLAGM"
     gene            2032..2799
                     /locus_tag="PLY05_09040"
     CDS             2032..2799
                     /locus_tag="PLY05_09040"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HNC03483.1"
                     /translation="MKIKERVIKNTWLLVEKICDYYLDKQLKAYVPPQKKHLIEPDYQ
                     QQIINFITQNPVNCSTVYLLEIMPEIVVTSTMVGRIGGAAIGVSQAQWPCYQQQPMDH
                     VITIDLSETPELKHGKLSNARAIAVFIKDKIDNQAYEPLTNEVSIRILSEQDIQQGEY
                     SPQQTDAATAYQLVPFVVPDFVFKEFYLLSTQEKEIKQAWDHIWSAYIGGQATWIQNE
                     EYEGQFIGQLSDLTDELNLGDNGVLYIFANTAFWQCH"
BASE COUNT          926 a          496 c          596 g          827 t
ORIGIN      
        1 ctatttaaat attgagcatc taacactaag gcagagtggg gctgattaaa ataaatggga
       61 caacgacaaa gttgctcata aatagggtga atgtgttgat tttctaaggc aggatcatgg
      121 ctaaagcgaa tggctttgag tgcttgtgga cattgacgtt tgaccaaaat ggcacaagcc
      181 ataatatatg tttcatcaca ctgacggaca aaaaataaat catcagactg atggcgcacc
      241 gtccacaaca ccacaccagg ttcatgaatc agcataggcc gagacaccgt cgacactaaa
      301 tggccaaact catttaaggc atgaaacatc tctagcaatg ttggtgaaga ctgcaccata
      361 taccctaaaa cacccaacat tgcaggttca gccattttta aaaaacgcaa acccaataaa
      421 ggatcaggat aatacaaaaa tgttgattgt gataaacgct ctaaaatatc ttggttgacc
      481 cagccatgac tagggttaag gtcggtaaat tgcacattcg cttcgcgtaa aagctgttca
      541 aaaggaaaac caatacgacc actcagccga atgtaatgtt ggataagaaa agaggcgaca
      601 acccctttag ttgttaattg catattcata gtgattattt ttttttgaat aagcagaaaa
      661 ggcgttgatc aagtcaactg aacaaaaatt atctactact aaagattgta aagtaaagcg
      721 tcttgtcaaa aaacgttaaa attttgttta aagatgtcat ggcctttttt gtgcgccgcc
      781 atgtccgttt ttgtcgttta ttttgtgccg ttcgtcggga tacactgcct accagttcac
      841 ggtatgttgc taacagcata acgggctaaa acaaaaaact ggactgatgc attctaccag
      901 aattagtgtg ttttgtgagc ataaaacgac tggatgtggt ttttatgtca acgaatgcag
      961 taattgcatg agtctgaata aaacttcagg gcggacacta tgaaaaatat tattactgca
     1021 acaggtatcg ttttaagctt attagcagca gcacctacac aagcagcttc tacctattca
     1081 caaaccaaat atcccgtcgt tttagcgcat ggcatgatgg gttgggacac catgttaggg
     1141 attgagtatt ggtatcaaat tcctaatgat ttacgtgaca atggtgcaag tgtttacact
     1201 actcaagcct ctggtttaga aagctctgaa gtccgtggcg agcagctctt aaaacaggtt
     1261 aaagatattt tagcgattac aggtaaaggt aaagtgaact tgattggcca tagtcatggt
     1321 ggtcattcag tgcgttatgt tgcagcaatg attcctaata atattgcctc agtgacgaca
     1381 atggcaacac ctgcgaaggg cgcaccaatg gccgatttga ttattaatgg tggctcatta
     1441 ggcagtgttg gcacaaccat tattcaagcc gcaggaacaa tgttgaattt attaaatggt
     1501 ggcagcaaat taggtttgga tgtgaacgcg aataagagct tggcttcttt gagcaccaaa
     1561 ggttctgccg agtttaatgt taagtttcct caagcaattc caaccacagc atgtggtgag
     1621 ggtgagtatg aggtgaatgg tgttaagtat ttctcttttg ctggcacatc accaacaact
     1681 aatgcgttag atttgcttga tcctgccgtg aagttagttt ccttagcatt taccaatggt
     1741 gaagccaatg atggttttgt gggacgttgt agtgctcacg ttggtaaagt ggtgcgtgat
     1801 aactataata tgaaccacat tgattttatg aatcatatct tcggtttacg tggtttaaca
     1861 accgatccaa aagcagtgta tcgtgaacaa ttaaaccgcc taaaattagc aggtatgtaa
     1921 ttttgttgat gatgtagggc ggcttaggcc gcctttttta ttgagttaat atagggcttg
     1981 ataaacattg gttttgttag gagaataaag ttttactatg gagtaaacag tatgaaaatt
     2041 aaggaaagag tgataaaaaa cacatggttg ttagtagaaa aaatatgtga ttattatctc
     2101 gataaacagc ttaaggctta tgtacctcct caaaaaaaac atctcattga acctgattat
     2161 cagcaacaaa ttatcaactt tattacacaa aacccagtga attgttcaac ggtatattta
     2221 ttagaaatta tgcctgaaat agtggttacc tcaacaatgg tagggcgtat tggcggtgct
     2281 gcaattggag tgagccaagc gcaatggcca tgttatcaac aacagcctat ggatcatgtc
     2341 attacgattg atttatcaga aacacctgaa ctaaaacatg gaaaattatc aaacgcacga
     2401 gcaatcgctg tttttattaa agataaaata gataatcagg cttatgagcc gttgaccaac
     2461 gaagtctcta tacgcatttt atcagagcaa gacattcaac agggtgaata tagtcctcaa
     2521 caaacagatg cagcaacggc ttatcaactt gtaccatttg ttgtgcctga ttttgttttt
     2581 aaagaatttt atttattatc aacacaagaa aaagaaatta agcaagcatg ggatcatatt
     2641 tggtcagctt atattggggg acaagctaca tggattcaga atgaggagta tgaagggcaa
     2701 tttattggcc aactatctga tttaactgat gaattaaatt taggtgataa tggcgtttta
     2761 tatatatttg caaatacagc cttttggcaa tgccattaat gataaactga tacgaaaaat
     2821 aatctttcaa agcgcaaaac atgac
//
LOCUS       DAOQMB010000296         2835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20462_length_2835_cov_14.248561, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000296 DAOQMB010000000
VERSION     DAOQMB010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20462_length_2835_cov_14.248561"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PLY05_09045"
     CDS             <1..663
                     /locus_tag="PLY05_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03484.1"
                     /translation="MSIKIAPAPWQLTGQGYLMVVHLPQDFLDNQSFIEPHLQASRKG
                     HLAYVAFADYQQSDVGPYHELLYVGGQLKIGQKHHYSISKIYVSTQDSIDNGQANWGI
                     PKEKAYFAVDYGQDKQDHIKVTTETGEVIANLRFSHHLLSFPVNTRLVPKALRTLAQE
                     WQGQRFYFPLEAQGHMKPALIKESFFDQRYFPDLNQGRVLACFKVTDFSLVFPVPKTQ
                     AV"
     gene            777..1655
                     /locus_tag="PLY05_09050"
     CDS             777..1655
                     /locus_tag="PLY05_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03485.1"
                     /translation="MINALKQTQRFLGLGDIPAHKLKARYSEDAYYLHVQGVDVRIKE
                     SGQGEPLILLHGFSASADTWDGWRKELSDQFRVIAIDVPPFAITGPLPVGKTTPNAVV
                     HFINAVTERLGLKQFYLGGNSLGGYLSWRYTVHYPTKVKKLILVDSAGYSMPTPAAVL
                     LTRHQLTRHFVKFVTPLPFVVQSVRSVYGDSSRIPRGTIRRYQDLLRREGTRAAIADM
                     MRGIITDSPDIKLIRTPTLVIWGAKDTWIPLKHGHQFQRDIANAKLVIYDDLGHIPME
                     EDPKRTAADARHFLTR"
     gene            1652..>2835
                     /gene="rhlB"
                     /locus_tag="PLY05_09055"
     CDS             1652..>2835
                     /gene="rhlB"
                     /locus_tag="PLY05_09055"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HNC03486.1"
                     /translation="MSDTNLATFNQFQLSPLLQRAIADLGFSHCTPIQSQVLHFTLAG
                     HDAIGRAQTGTGKTAAFLITLINDLLKIPPSEDRYLGETRALVLAPTRELALQIAKDA
                     EDLAKYSKLRIVTLVGGMDYEKQKQQLYSDFVDIVVATPGRLIDFVEQKAIYLDQLDV
                     LVIDEADRLLDMGFIPAIKRIVRACPPKTHRQTLFFSATFNQDVLNLADQWLFNPVKV
                     EIEPAQVTNETVQQVVYIVSAEQKYDLLYNLIQLDNFERVIVFANRRDQVRRLADMLK
                     HHRISCAVLSGEVPQEKRVKTLENFREGRVQVLVATDVAGRGIHIDDVSHVINFTLPE
                     QTDDYVHRIGRTGRGGASGVSISFACEDDAFLIPNLEAAIGRKLDCTHPPAELLQKPR
                     GR"
BASE COUNT          812 a          548 c          614 g          861 t
ORIGIN      
        1 atgagcatca aaattgcgcc tgcaccgtgg cagttaacag gacaaggtta tttaatggtg
       61 gtgcatttgc cccaagattt tttggataat caaagtttta ttgaaccgca tttacaagct
      121 agtcgcaaag gtcatttggc ttatgttgct tttgctgatt atcaacaatc ggatgttggc
      181 ccttatcacg aattattata tgtgggtggg cagctaaaaa ttggccaaaa acatcattat
      241 tctatttcaa aaatttatgt gtccacacag gactcaattg ataatggtca agcgaattgg
      301 ggcattccca aagaaaaagc ctattttgcg gttgactatg gtcaagataa acaagatcac
      361 attaaagtca ccaccgaaac aggagaggtg attgctaatt tgcgttttag ccatcattta
      421 ttgtcgtttc ctgttaatac ccgtttagta ccaaaagctt tgcgtacctt ggctcaagag
      481 tggcaaggcc aacgttttta ttttccttta gaagcacaag gacacatgaa acctgcttta
      541 attaaagaaa gtttttttga ccaacgctat tttccagact taaatcaagg acgtgtattg
      601 gcttgtttta aggtcacaga tttttcttta gtattccctg tcccaaaaac acaagcagtt
      661 tagccataac aatacaaccg caattggcgt attttgtcct taaattagta cgttggtaca
      721 aatccctcac ataaaaagaa agtgaggggg caatgataaa aatagaggtt cttatagtga
      781 taaacgcact caaacaaact cagcgatttt taggcttggg tgatattcca gcacacaaat
      841 taaaagcacg ttatagcgag gacgcttatt atttgcatgt acaaggagtt gatgtgcgta
      901 ttaaagaaag tggccaaggt gagccattaa ttttattaca tggtttttcg gcttcggctg
      961 atacatggga tggatggcgt aaagagttaa gcgaccagtt tcgcgtcatt gccattgatg
     1021 tgccgccatt tgccattact ggcccattac ctgtgggtaa aaccacaccc aatgccgttg
     1081 tccattttat taatgcggtg actgaacgtt tgggtttaaa gcagttttat ttaggtggaa
     1141 attctttagg tggttatttg tcttggcgtt ataccgttca ttatcctacc aaagttaaaa
     1201 aattaatttt agtagatagt gcaggttatt cgatgccaac gccagcggca gtattattaa
     1261 cgcgtcatca attaactcgt cattttgtta agtttgttac gccattaccg tttgtggtgc
     1321 aaagtgtacg ttctgtatat ggtgatagca gccgtattcc tcgtggaacg atacgccgtt
     1381 atcaagattt attgcgtaga gaggggacac gcgcggcaat cgctgatatg atgcgcggta
     1441 taattaccga ttcacctgat attaagttga ttcggacgcc aacccttgtg atatgggggg
     1501 caaaagacac atggattcct ttaaaacacg gtcaccaatt ccaacgagat attgccaacg
     1561 ctaagttagt gatttatgac gacttagggc atattccgat ggaggaagac cccaaacgaa
     1621 cggcagccga tgcccgacac tttttaacac gatgagcgat actaatttag ccacttttaa
     1681 ccagttccag cttagcccgt tattacaacg ggcaattgcc gatcttggat ttagccactg
     1741 cacccccatt cagtctcaag ttttacattt tacccttgca ggccatgatg ccattggtcg
     1801 agcgcaaaca ggcacaggta aaacagcggc cttcttaatt accttaatta atgacttatt
     1861 aaaaataccg ccttctgaag atcggtattt aggtgaaact cgcgctttag tgttagcacc
     1921 cacccgcgag ctagccttac aaattgctaa agatgctgaa gatttagcca aatacagtaa
     1981 attgcgtatt gtcaccttag ttggtggcat ggactacgaa aagcaaaaac aacaacttta
     2041 tagtgatttt gttgatattg tggtggctac accaggtcga ttgattgatt ttgttgagca
     2101 aaaagccatt tatttagatc aattagatgt gttggtgatt gacgaagccg atcgtttatt
     2161 agacatgggc tttattcctg cgattaaacg tattgtccga gcttgcccac ccaaaaccca
     2221 tcgccaaact ttatttttct cggcaacttt taatcaagat gtgttaaatc ttgccgatca
     2281 atggttattt aatcctgtta aagtcgaaat tgagccagca caagtcacta atgaaaccgt
     2341 acaacaagtg gtttatattg tcagtgccga acaaaaatac gatttgttat ataacttgat
     2401 tcaattagat aattttgagc gagtgattgt ttttgctaat cgccgcgatc aagtgcgccg
     2461 tttagcggat atgctcaagc atcatcgtat ttcttgtgcg gtgttatcag gcgaagtgcc
     2521 acaagaaaag cgcgttaaaa ccctcgaaaa tttccgtgaa ggtcgtgtgc aagtattagt
     2581 tgccaccgat gtcgcagggc gtggtattca tattgatgat gtcagccatg tgattaactt
     2641 taccttaccc gaacaaaccg atgattatgt gcatcgtatt ggtcgaacag gtcgtggtgg
     2701 ggcaagtggg gtgagtatta gttttgcttg tgaagatgat gcctttttga ttcctaattt
     2761 agaggcagca attggtcgta aattggattg tacacatcca cctgcggagt tgttgcaaaa
     2821 gcctagagga agata
//
LOCUS       DAOQMB010000297         2834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20479_length_2834_cov_15.074127, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000297 DAOQMB010000000
VERSION     DAOQMB010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20479_length_2834_cov_15.074127"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="PLY05_09060"
     CDS             complement(<1..615)
                     /locus_tag="PLY05_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03487.1"
                     /translation="MIKPLFLMDLDDTLLQTARKMPADMPRQVAALDKHGQPLSFTNP
                     KQQAFIDWLLLCADVVPVTARSVDAFKRVQIPFKQGAICAHGAVILQANGKLDKDWHA
                     LMQQRLNPYLSLLDDIEKTLASIGQQLNLTLRTWLVEEQGLACYALAKQSGHHDQVLA
                     DVEQKIKQQFDLKDFYIHRNANNLAILPKVLHKQFAVEYWLQQDE"
     gene            complement(608..1717)
                     /locus_tag="PLY05_09065"
     CDS             complement(608..1717)
                     /locus_tag="PLY05_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004927253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HNC03488.1"
                     /translation="MTSSFLVQLSRGTLNVSVEQASIAPEQLFDFAERRNPKRAFLFV
                     SKVLGRHIPVKPSLMAQAFNQLAAQIPDHLPEPVLVIGMAETAVGLGAGVHRALQQRY
                     PNAMYWMSTRHSLGTPLFARFEEEHSHASSHLLHWPVDEALKQQLLASKTLIIVDDEA
                     STGKTFINLAQALQDAGLQQIERIIAATLTDWSGDAVQAKLGKKAQTISLLSGSYQWQ
                     ADLNAPLPEMPKVDSVAQGAWQPNLALDWGRLGVIQHQATLNDKVSVDKGEKILVLGT
                     SEYVWRSFLLAEHLEQQGAEVYFASTTRSPIAVGHAIKIARAFSDNYGLGIANFSYNV
                     DVNQYDKIMICSETPAAMVDQALLVELKAQVIVDD"
     gene            complement(1767..2399)
                     /locus_tag="PLY05_09070"
     CDS             complement(1767..2399)
                     /locus_tag="PLY05_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018676840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03489.1"
                     /translation="MNKYNSNIHHRRSIRLKGYDYAQAGLYFITICCQDRAHLFGEII
                     NEVMVLNEYGKIAFTEWENSMNIRDNIHLHDFVIMPNHIHGIIEIVFKKANQDAEELE
                     IAPITKFQSPSQTIGSIVRGYKIATIKKIKNKVLEESSMQCKGECKGELQFALTHTFA
                     LTEKIIQLDFKIWQRNYYEHIIRDEASHKRISDYILNNPAQWTDDKFYQT"
     gene            complement(2488..>2834)
                     /locus_tag="PLY05_09075"
     CDS             complement(2488..>2834)
                     /locus_tag="PLY05_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HNC03490.1"
                     /translation="GQLPVLNLYSASQEGRFLPDNQRLMVYNNQSLYQWHSNKLLFPN
                     ERLTPEQKRRVGYFVFHQQQWYLVNEALPDLMNVASKQTIAIGDKIALVDGLQLLLSR
                     GEGSRVVVVQMV"
BASE COUNT          861 a          600 c          496 g          877 t
ORIGIN      
        1 ttcatcctgt tgtaaccaat attcaacggc aaattgttta tgcaatactt taggcaaaat
       61 tgctaaattg ttagcgtttc gatgaatata aaaatctttt aaatcaaact gttgctttat
      121 cttttgctca acatcagcca acacctgatc gtgatgacca ctttgtttgg ctaaggcata
      181 acacgctaag ccttgctcct caaccaacca tgttcttaaa gttaaattaa gttgctgacc
      241 aatactagct aaggttttct caatatcatc taacaaactt aaatacggat ttaaacgttg
      301 ttgcattaaa gcatgccaat ctttatccaa tttaccattg gcttgtaaaa tgactgcacc
      361 atgcgcacaa attgctcctt gcttaaaagg aatttgcact cgtttaaaag catccacact
      421 acgcgccgtg acaggcacaa catcggcaca aagcaacaac caatcaataa aagcttgttg
      481 tttgggatta gtaaaactta aaggctgtcc atgtttatct agagcggcga cttgtcttgg
      541 catatcagca ggcattttgc gtgccgtttg caacaaggtg tcatctaaat ccattaaaaa
      601 tagaggttta atcatcaact atcacctgtg cctttaactc tactaacaat gcttgatcga
      661 ccattgccgc aggtgtttcg ctgcaaatca taattttatc atattgatta acatcaacat
      721 tataagaaaa attagcaatg cccaagccat aattatcact aaaggcacga gctattttaa
      781 tggcatgacc cacagcaatc ggtgagcgtg tggttgaagc aaaatacacc tctgcaccct
      841 gttgttctaa atgttcagct aataaaaatg atcgccaaac atattcactt gtgcctaaca
      901 ctaatatttt ttcaccttta tctactgaca ctttatcatt taaagtagct tgatgctgaa
      961 ttactcccaa acgtccccaa tctaaagcta aattgggttg ccacgcgcct tgagctactg
     1021 aatcgacttt cggcatttcg ggtaatggtg cattaagatc agcttgccat tgatatgaac
     1081 ctgacaataa ggaaatagtt tgggcttttt tgcctaactt tgcttgcacc gcatcacccg
     1141 accaatcggt taaagtcgca gcaataatac gctcaatctg ttgcaaacct gcgtcttgta
     1201 aagcttgtgc caaattaata aaggttttac ctgttgaggc ttcgtcatcg acaataatta
     1261 aagttttgct ggctaatagt tgttgtttta aggcttcatc aacgggccaa tgcaataaat
     1321 gagaactggc atgactatgc tcttcttcaa aacgcgcaaa caatggtgtt cccaaactgt
     1381 gacgagtact catccaatac atggcgtttg ggtagcgttg ttgtaaagcg cgatgcacac
     1441 ccgcacctaa acccaccgcc gtttcagcca taccaatcac caagacaggt tcaggcaaat
     1501 gatcaggaat ttgtgctgct aattgattaa aagcctgtgc catcaatgaa ggtttaacag
     1561 gaatatgtcg gcctaagact ttagagacaa acaaaaacgc ccgtttagga ttacgccgct
     1621 cagcaaaatc aaataactgc tcgggcgcaa tactcgcttg ctcaacgctg acgtttaatg
     1681 tgccacgact gagctgtacc aaaaaagaag aagtcatatt tgccacttgg ttaaaatttc
     1741 atggctaatt ttaacagtaa ataggattat gtttgataaa acttgtcgtc tgtccattgg
     1801 gcaggattat taagaatata atctgaaatg cgcttatggg atgcttcatc acgaataatg
     1861 tgttcgtaat agttacgttg ccatatttta aaatctaatt gaataatttt ttctgtcagg
     1921 gcaaaggtat gggtaagggc gaattgcaat tcgcccttac attcgccctt acattgcata
     1981 ctactttcct ctaaaacttt attttttatt tttttaatag tggctatttt gtagcctctg
     2041 acgatagagc ctatagtttg tgatggtgat tgaaattttg tgattggagc aatttctaac
     2101 tcctctgcat cttgattggc ttttttaaaa acaatttcaa taatgccatg aatatgattg
     2161 ggcataataa caaaatcatg caagtgaata ttatctctaa tgttcatcga gttctcccac
     2221 tctgtaaaag ctatttttcc atattcattt aacaccatga cttcattaat aatttcacca
     2281 aataaatgcg ctctatcctg acagcatatt gtaatgaagt acaatcctgc ctgtgcatag
     2341 tcgtatcctt ttaatcggat agatctacga tgatggatat tggaattgta tttattcatc
     2401 ttttttcctt gttggggcat tagtatgggc gaattgcaat gctatagtac gggcgaattg
     2461 caattcgccc atacttacaa ttcgctccta caccatctgt accaccacta cccgactacc
     2521 ctcaccgcgt gataacaaca attgcaaacc atccaccaac gcgattttat caccaatagc
     2581 aatcgtttgt ttactggcca cattcatcaa atcgggcaaa gcctcattga ccaaatacca
     2641 ttgctgttga tgaaaaacaa aataaccgac tcggcgtttt tgttcaggtg tcaaacgttc
     2701 attaggaaat aataatttat tgctatgcca ttgatataac gattgattgt tatagaccat
     2761 caaacgctga ttatcaggca aaaatcgtcc ttcttggctg gcagagtaca aatttaacac
     2821 aggtaattgg ccaa
//
LOCUS       DAOQMB010000298         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20494_length_2833_cov_14.000360, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000298 DAOQMB010000000
VERSION     DAOQMB010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20494_length_2833_cov_14.000360"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2833)
                     /locus_tag="PLY05_09080"
     CDS             complement(<1..>2833)
                     /locus_tag="PLY05_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HNC03491.1"
                     /translation="IVPAEIKALHEYGVTKIFSPEDGATLGLQGMINLCAQGADQDLA
                     HQYPVDFADLQAADVMLRQRTLARIISALEADVLPYKKELLAAAVDKKTPVLGITGTG
                     GAGKSSLTDEILRRFRLDFNDSINIAIISIDPSRRKSGGALLGDRIRMNAIHHPNMYM
                     RSLATRSTGGEISQALPDVIAACKVANFDLIIVETSGIGQGDAAIVPYVDLSLYVMTP
                     EFGAASQLEKIDMLDFADIVAINKFDRKGAKDALRDVCKQVQRNREAFMQMPETMPVF
                     GTIAARFNDDGVTALYQQLKADLISKGWVAPKNSQLPVVSVRSSSQQQSIVPPAKVRY
                     LAEIADAVRTYHHYVEQQAQLARQRQQLQATQLMLKDAPSPVGEGWGEGLTQLIEQKD
                     AQLSHESKQLLARWSELKQRYSQDELVVKIRDKELRTKLTYTSLSGNKIPKVALPKFH
                     DAGDILAWQLRENIAGEFPFTAGVFPFKREGEDPTRMFAGEGDAFRTNARFKKVSEGM
                     PAKRLSTAFDSVTLYGNDPAERPDIYGKIGNSGVSIATLEDMKVLYGGFDLTHPMTSV
                     SMTINGPAPTILAFFLNTAIDQNIEKFVAKEQRQPDDAELANIKQWTLENVRGTVQAD
                     ILKEDQGQNTCIFSTEFSLKVMGDIQQYFVEHNVRNFYSVSISGYHIAEAGANPISQL
                     AFTLANGFTFVEAYLARGMSIDDFAPNLSFFFSNGMDPEYTVLGRVARRIWAVTMREK
                     YGANERSQKLKYHIQTSGRSLHAQEIDFNDIRTTLQALIAIYDNCNSLHTNAYDEAIT
                     TPTEESVRRAMAIQLIINREWGLAKNENPNQGSFIIDELTDLVEEAVLKEFEAISERG
                     GVLGAMETGYQRGKIQEESMYYEHLKHSGEYPIIGVNTFRNPKGDATPQSLELARSTD
                     SEKQSQLQRLADFQHRYAQQAPQALARLQQ"
BASE COUNT          781 a          668 c          583 g          801 t
ORIGIN      
        1 cttgttgcaa acgtgctaag gcttgtggtg cttgctgggc ataacggtgt tgaaagtccg
       61 ccaaacgttg taattgcgat tgtttttcgc tatcggttga acgcgccaac tccaaacttt
      121 gtggtgtggc atcgccttta ggattacgga aggtattcac accaataatc ggatattcgc
      181 cactgtgttt taagtgttcg taatacatag actcttcttg aatcttgcca cgttgataac
      241 ctgtttccat tgcgcccaac acgccaccac gctcactaat tgcttcaaac tcttttaaca
      301 ccgcttcttc gactaaatca gtcaactcgt cgataataaa actaccttga ttggggtttt
      361 catttttcgc caaaccccat tcacggttaa taatgagctg aatcgccatt gcccgccgca
      421 cactttcttc ggtaggtgtg gtaatcgctt cgtcataagc attggtgtgt aaactattgc
      481 agttatcata aatggcaatc aatgcctgta acgtggtgcg aatatcatta aaatcaattt
      541 cttgggcgtg taaactacga ccgctggttt gaatatgata tttgagtttt tgactacgtt
      601 cgtttgcgcc atatttttcg cgcatggtca ctgcccaaat acgccgagcg acacggccta
      661 acacagtata ctcaggatcc ataccattgc taaaaaagaa cgataaattc ggcgcaaaat
      721 catcaatact catgccacgc gctaaataag cctcaacaaa ggtaaaacca tttgctagcg
      781 taaaggctaa ttgcgaaatg gggttcgcgc ctgcttcggc aatgtgatag cccgaaatag
      841 acaccgagta aaaattacgc acattatgtt caacaaaata ttgctgaata tcacccatca
      901 cttttaagct aaattcggta gaaaaaatac aggtattttg accttggtct tcttttaaaa
      961 tatcggcttg caccgtgcca cggacatttt ctagcgtcca ttgtttaatg tttgccaatt
     1021 ctgcatcgtc gggttggcgt tgttctttgg cgacaaattt ttcaatattt tggtcaatcg
     1081 cggtgtttaa aaagaacgcc aaaatagtcg gtgctgggcc attaatggtc atcgacaccg
     1141 aggtcatggg atgggttaaa tcaaaaccac catacaagac tttcatatct tctaaagtcg
     1201 caatcgacac gcccgaatta ccaattttgc cataaatatc aggacgctca gcaggatcat
     1261 taccatacaa agtgactgag tcaaacgcgg ttgataaacg tttagcaggc ataccttcgg
     1321 aaactttttt gaagcgtgca ttggtacgaa aggcatcacc ttcacccgca aacatccgtg
     1381 tcgggtcttc accctcacgt ttaaacggaa acacccctgc ggtaaaaggg aactcccctg
     1441 caatattttc gcgtaattgc cacgctaaaa tatcacctgc atcatgaaac tttggcaaag
     1501 ccactttggg aattttgttg cctgataagc tggtataggt cagtttggta cgcaattctt
     1561 tatcacgaat tttaaccacc aattcatctt ggctataacg ttgttttaac tccgaccaac
     1621 gggctaatag ttgtttagat tcatggctga gttgtgcgtc tttttgctcg atgagttgcg
     1681 ttaagccctc accccaaccc tctcccacag gagagggagc atcttttaac atcaattgag
     1741 tcgcttgtaa ttgctgacgt tggcgcgcca attgtgcttg ttgctcaaca taatggtgat
     1801 aagtacgcac tgcatcggca atttcggcta aataacgcac ttttgcaggt ggcacaatgc
     1861 tttgttgttg gctcgaacta cgtacactca ccacaggcaa ttgactgttt ttgggtgcta
     1921 cccaaccttt agagattaaa tcggctttga gttgctgata taacgccgtg accccatcat
     1981 cattaaaacg cgccgcaatc gtgccaaaaa cgggcatggt ttcgggcatt tgcataaagg
     2041 cttcacgatt tcgttgcact tgtttgcaca catcacgtaa ggcatctttt gcgcctttgc
     2101 ggtcaaactt gttaatggcg acaatatcgg caaaatcgag catatcaatt ttttcgagct
     2161 gtgaggccgc accaaactca ggggtcatga catataagga caaatccaca taaggcacaa
     2221 tcgccgcatc accctgacca atgcccgacg tttccacaat aatcaaatca aaattggcca
     2281 ctttgcaagc ggcaatcaca tcgggcaaag cttgcgaaat ttcgccacct gtactgcgtg
     2341 tggccaacga acgcatatac atattaggat gatgaatggc attcatacgg attctatcgc
     2401 ccaataatgc gccacctgat ttacggcgtg atgggtcaat cgaaataatg gcaatattaa
     2461 tgctatcgtt aaaatctaaa cgaaaacggc gtaaaatctc atcggttaaa ctcgactttc
     2521 ctgcaccacc tgtacctgta atccccaaaa cgggtgtttt tttatcgacc gcagccgcta
     2581 gtaattcttt tttataaggt agaacatcgg cttctaaggc agaaataatc cgcgctaagg
     2641 ttcgctgacg caacatgaca tcagccgctt gtaaatcggc aaaatcaaca gggtattgat
     2701 gggctaaatc ttggtctgca ccttgcgcac aaaggttaat catgccttgt aaaccaaggg
     2761 ttgcaccgtc ttctggggaa aaaatcttcg tcacgccata ctcatgtaag gctttaattt
     2821 cggcaggcac aat
//
LOCUS       DAOQMB010000299         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20495_length_2833_cov_13.771778, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000299 DAOQMB010000000
VERSION     DAOQMB010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20495_length_2833_cov_13.771778"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLY05_09085"
     CDS             <1..434
                     /locus_tag="PLY05_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008306328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC03492.1"
                     /translation="DYAHHMVLPTICLTIGGFATLTMLTKNAFLDEIHKQYVTTAKAK
                     GLNDSQVLYGHVFRNAMLIIIAGFPAALLGVFFTGSFLVEVIFNLDGLGLLSFESVIN
                     RDYPVIFGTLFIFTLIGLVLRIISDVTYMLVDPRIDFNSRG"
     gene            437..1456
                     /locus_tag="PLY05_09090"
     CDS             437..1456
                     /locus_tag="PLY05_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNC03493.1"
                     /translation="MALSPLNQRRIANFKANKRGYYALWLFLVLFVFSLFAEFWANDK
                     PLLVSYKDSLYTPIFKDYPETTFGGTLNSNTDYRDPYVAGLINEQGWMIWPPIRFSYS
                     TINYNLTVPAPAPPSADNWLGTDDQGRDVLARIIYGFRISILFALTLTIGSSVVGIIV
                     GAVQGFYGGWVDLIGQRIIEIWSGLPMLFMLIIMASLVEPNFWWLLGLMLLFSWTELT
                     GLVRAEFLRGRNLEYVRAARALGLSDSKIMFRHILPNAMVSTLSFLPFIFTGAIGLLT
                     SLDFLGFGLPVGSPSLGELVAQGKNNMSAPWLGLSAFFTLAILLTLLVFIGEAVRDAF
                     DPRKQ"
     gene            1431..>2833
                     /locus_tag="PLY05_09095"
     CDS             1431..>2833
                     /locus_tag="PLY05_09095"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HNC03494.1"
                     /translation="MPLTPANNDPLLVVNDLHVRFYNTGTQSTEPVKGVSFSLHQGEM
                     LAIVGESGSGKSLTAQSIMRLLPDSARYQGQILFNGEDVLTVPIERLQQLRGDKIGMI
                     FQEPMTSLNPLHTIEKQISENILQHQQLSKAQAQQRTLELLKEVGIPEPEKRLNSYPH
                     ELSGGQRQRVMIAMALANNPKLLIADEPTTALDVTLQEQILHLLQQLQAKFGLAVLLI
                     SHDLSLVRRFAQRVAVMQHGLMVEIKATQQLFAEPEHPYTQMLLAAEPDGQPTPVAED
                     AATILKIDQLRLWFPIKQGFWQRVVGHIKAVDGIDLHLKAGETLGVVGESGSGKSTLA
                     FAITRLSPITEGTIIFAGQDIVTLSQKQLRPLRKDLQIVFQDPYGSLSPRLSVGQIIS
                     EGLELLGLAKEEQEQRVIEALTAVELDPNSRHRYPHEFSGGQRQRIAIARALVLKPKL
                     LILDEPTSALDRTVQKQ"
BASE COUNT          745 a          574 c          604 g          910 t
ORIGIN      
        1 tagattatgc tcaccacatg gtcttaccca ccatttgttt aaccattggc ggttttgcta
       61 ccctaaccat gctcaccaaa aatgcttttt tagatgaaat tcataaacaa tatgtcacta
      121 ccgctaaagc taaaggacta aatgacagtc aagtgttata tggtcatgtt tttagaaatg
      181 cgatgttaat tattattgct ggctttcctg cggcattatt aggcgtattt tttactggct
      241 catttttggt tgaagtcatt tttaacttag atggtttggg tttattaagt tttgaatcag
      301 tgattaaccg tgattaccct gttatttttg gcactttatt tatttttacg ctgattggtt
      361 tagtgctgcg tattattagt gatgtgacat atatgttagt tgatccacgc attgatttta
      421 atagtcgggg ataatcatgg ctttatctcc tcttaatcaa cgacgtattg ccaactttaa
      481 agccaacaaa cgtggttatt atgcgttatg gctattttta gtgctttttg tatttagttt
      541 atttgccgaa ttttgggcaa atgataaacc gttattagtc tcgtacaaag acagtttata
      601 cacaccaatt tttaaagatt atcccgaaac cacctttggc ggtacgctta acagtaacac
      661 cgattatcgt gacccttatg ttgcgggttt aattaatgag caaggttgga tgatttggcc
      721 accgattcgt tttagttata gcaccattaa ttacaatctc accgtccctg cacctgcgcc
      781 gccttctgcc gataattggc tcggtacaga tgaccagggc cgagatgtct tggcgcgcat
      841 tatttatggt ttcagaattt cgattttatt tgctttaacc ttaactattg gtagctctgt
      901 ggttggtatt attgttgggg cagttcaagg cttttatggc ggatgggttg atttaattgg
      961 tcaacggata atcgaaattt ggtctggcct accgatgtta tttatgctga ttattatggc
     1021 gagtttggtt gagccaaatt tttggtggtt gttaggttta atgttgctgt ttagctggac
     1081 ggaactgaca ggtttagttc gtgccgagtt tttgcgaggg cgtaatttag aatatgttcg
     1141 tgccgcacgc gctttgggac tcagtgatag caaaattatg tttagacaca ttttgcccaa
     1201 tgccatggtt tctaccctgt cttttctgcc ctttattttt acaggtgcga ttggtttatt
     1261 aacttctcta gacttcttag gttttggttt acccgttggc tcaccgtcgt tgggggaatt
     1321 agttgcccaa ggcaaaaata atatgagtgc gccttggttg ggtttatcgg ctttttttac
     1381 tttagccatt ttattaacgc ttttagtttt tattggtgag gcggtacgtg atgcctttga
     1441 cccccgcaaa caatgatcca ctgttagtgg tcaacgattt acatgtgcgc ttttataata
     1501 caggcacaca aagcactgaa cctgtcaaag gcgtgagctt tagccttcat caaggagaaa
     1561 tgcttgcgat tgtgggtgaa agtggttcgg gtaaatcctt aactgcgcaa tctataatga
     1621 gattattacc cgattcagca cgttatcaag ggcaaatttt atttaatggt gaagatgtcc
     1681 taaccgttcc tatcgaacgt ttacaacaat tacgcggtga taaaattggc atgatttttc
     1741 aagagccaat gacctcactt aacccgttac ataccatcga aaagcaaatt agcgaaaata
     1801 tcctgcaaca ccaacaactc agtaaagcac aagcgcaaca acgcacctta gaattactaa
     1861 aagaagttgg cattcctgag cctgaaaaac gtcttaacag ctatccacat gagttatcag
     1921 gcggtcagcg gcaacgggtg atgattgcca tggccttagc caataatcct aaattattga
     1981 ttgctgatga gccaaccact gcccttgatg tgacattaca agagcaaatt ttgcatttat
     2041 tacaacagct acaagccaaa tttggtttag cagtgctgct aatttcccat gatttaagtt
     2101 tagttcgacg ttttgctcaa cgtgttgcgg tcatgcagca cggtttaatg gtcgaaatta
     2161 aagccacaca gcagttattt gctgaacctg aacatcccta cacccaaatg ctcttagccg
     2221 ctgagcctga tggacagcca acacctgtgg ccgaagatgc tgccactata ctcaaaattg
     2281 accagctacg tttatggttt cccattaaac aaggtttttg gcaacgggtg gttggtcata
     2341 ttaaagccgt ggatggtatt gatttacacc tcaaagcagg cgaaaccttg ggtgtggtcg
     2401 gtgaaagtgg ctctggcaag tctaccctcg cctttgccat tacccgactc agccctatta
     2461 ccgaagggac gattatattt gcaggtcaag acattgtgac tttgtcacaa aaacaacttc
     2521 gtccgctacg caaagatcta caaatcgtct ttcaagaccc ttatggcagc ttaagcccac
     2581 gtttaagtgt cggtcagatt atcagtgaag gcttagaatt acttggtttg gccaaagagg
     2641 aacaagagca acgggtgatt gaagcattaa ctgccgttga gcttgatccc aactcacgtc
     2701 atcgttaccc ccatgaattt tcgggcggtc aacgtcaacg catagccatt gcgcgtgcgc
     2761 tggtgctaaa accaaagtta ttaattttgg atgaacccac ctcagcactg gatagaaccg
     2821 tacaaaaaca aat
//
LOCUS       DAOQMB010000300         2830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20550_length_2830_cov_12.916757, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000300 DAOQMB010000000
VERSION     DAOQMB010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20550_length_2830_cov_12.916757"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLY05_09100"
     CDS             complement(<1..440)
                     /locus_tag="PLY05_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC03495.1"
                     /translation="MNTLLNPTTSEVTAMLLPINAVERETGISKELLRMWERRYGFPQ
                     PQRDAQGDRVYPTEQINKLRIIRRLLDAGFRPSKVINLEMPALEELVGSSHGNPSPAV
                     PNNIESELLSILKGRDPYRVREYLNHQLIRMGLESFILDLVQHT"
     gene            complement(437..964)
                     /locus_tag="PLY05_09105"
     CDS             complement(437..964)
                     /locus_tag="PLY05_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HNC03496.1"
                     /translation="MKKFAAISASLLLSACMTNPMEQYKNTTPSLDLKQYLNGELEAW
                     GQFQDRSGKLIKRFHVDMTATWQGNKGVLDEKFSYDDGSKQQRIWYLEATGQGQYIGR
                     ADDVVGIAKGQVSGSVLNWQYTLALPVNGKIYHVNFDDWMYLHDDKTLVNRAVMSKFG
                     FRLGEVTLFFRKKSS"
     gene            complement(969..2174)
                     /locus_tag="PLY05_09110"
     CDS             complement(969..2174)
                     /locus_tag="PLY05_09110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNC03497.1"
                     /translation="MSIPSTLLSNQVPLKASLFIKMLDKLAYGSMRLTLPDGEKIFFN
                     GKHAGIEADLHIKNWLAVSKILQSGDIGVAEAYRDDWIDSDNMLNVLLLALANEQALD
                     KALYGSFLGTLIYRVKHWLNRNTRANSKKNIHAHYDIGNDFYRLWLDPSMTYSSAYFS
                     GQSQSLEQAQYAKYDRLLKQLNVKAGDHILEIGCGWGAFAEYAAREYGCFVTGISLSK
                     EQLAYAEQRIANKPYANQIHFKFCDYRDLRGKYDAIVSIEMLEAVGEQWWPTYFAKVA
                     SCLRQGGKAAIQTITISNERFDDYRLKTDFIQQYIFPGGMLPSPEKLYQQSQQANLTI
                     TDFVDFGLDYAHTLKLWRESFDAQLPQIRALGFDEAFIRIWRFYYWYCEAGFLTKRTG
                     VCQVLLTRE"
     gene            complement(2171..>2830)
                     /locus_tag="PLY05_09115"
     CDS             complement(2171..>2830)
                     /locus_tag="PLY05_09115"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HNC03498.1"
                     /translation="LSARHQLARAGFGYNRFNLLSFYEQDHGNGQDAEQWIKQILADK
                     NLDQIANGEIWLQSQPRVLGYVFNPVSFWYCYDQQQQLRVILCEVNNTFGERHCYLLT
                     ASHHQVIDNHTTLNCEKIFHVSPFFAVEGEYQFRFVHKKNTRSVAINYFVNQQLTLKT
                     VVTGQAQDLSLSNICQQLAKLGWTTVLVVARIHWQALKLWRKGAIFHRKPLPPQMEIS
                     S"
BASE COUNT          881 a          567 c          538 g          844 t
ORIGIN      
        1 ttagtatgtt ggactaaatc taaaataaat gactctaaac ccattctgat taattgatgg
       61 tttaagtatt cacggacacg ataagggtca cgacctttaa gaatggacaa taattccgat
      121 tcaatattat taggcacagc agggctagga ttaccgtgac tagagccgac taactcttct
      181 aaggctggca tttccaagtt aatcacttta cttggtctaa aacctgcgtc taataaacga
      241 cgaataatac gcagtttatt gatttgctcg gtgggataaa cacgatctcc ttgtgcatca
      301 cgttgtggtt gaggaaaacc ataacgccgc tcccacatgc gcaataactc tttagaaata
      361 cctgtttctc gttcaacagc attgatgggt agtaacatag ctgttacctc gctagtagta
      421 ggatttaata gggtattcat gaacttttct tccgaaaaaa taaggtgact tcacctaaac
      481 gaaagccaaa cttagacata actgcacgat taactaaagt tttatcatca tgcaaataca
      541 tccagtcatc aaaattaaca tgataaattt tgccattgac gggcaaagct aaggtgtatt
      601 gccaatttaa aactgaccca gaaacttggc ctttagcaat accaaccaca tcgtcagcac
      661 gaccaatgta ctgaccttgg cctgttgctt ctaaatacca aatacgttgt tgtttgctgc
      721 cgtcgtcata actaaacttt tcatctaaaa cgcctttatt gccttgccat gtggcagtca
      781 tatcaacatg aaaacgttta attaacttgc ctgagcgatc ttgaaactgt ccccaagctt
      841 ctaactcacc attgagatat tgctttaaat ctaagctagg ggtggtgttt ttatactgtt
      901 ccatgggatt agtcatacaa gcactcaata ataaggaagc actgattgca gcaaattttt
      961 tcatgatgtt attctcgggt gagtaacact tggcaaacac cagttcgttt ggttaaaaaa
     1021 cctgcctcgc aataccaata ataaaaacgc caaatacgaa taaaggcttc atcaaaacct
     1081 aaagcacgaa tttgaggaag ttgtgcatca aaactttcac gccataattt gagagtgtgc
     1141 gcgtaatcta ggccaaaatc gacaaagtcg gtgatggtta aattagcttg ttgactttgt
     1201 tgatagagtt tttcgggtga gggcagcatc ccaccgggaa aaatatactg ttgaataaaa
     1261 tcagttttta accgatagtc atcaaaacgc tcattactaa tggtaatggt ctgaatcgct
     1321 gctttgccgc cttgacgtaa acaactagct actttggcaa aataagttgg ccaccattgt
     1381 tcgcccactg cttctaacat ttcaatagag acaatcgcat catatttacc gcgcaaatca
     1441 cgataatcac aaaacttaaa atgaatttga ttggcataag gtttattagc aatacgttgt
     1501 tcggcataag ctaattgctc tttagataaa gaaatacctg tgacaaaaca accatattcg
     1561 cgtgctgcat attcggcaaa cgcgccccaa ccacaaccga tttctaaaat atggtcacct
     1621 gctttaacgt ttaactgttt gagtaaacgg tcgtatttgg cgtattgtgc ttgttctagg
     1681 ctttgagatt ggccactaaa ataagctgag gaataagtca ttgatgggtc gagccatagg
     1741 cgataaaagt cgttaccaat gtcataatga gcatgaatat tctttttact gttggctcta
     1801 gtattacgat ttaaccagtg tttaacccga taaattaatg ttcctaaaaa actgccatat
     1861 aaggctttat caagggcttg ctcattggct aaggctaata acaacacatt aagcatgttg
     1921 tcactatcta tccagtcatc gcggtaagct tcagcaacgc caatatcacc actttgtaaa
     1981 atcttactca cagcaagcca atttttgatg tgcaaatcag cttctatccc tgcgtgtttg
     2041 ccattaaaaa agattttttc tccatcaggt aaagtcagac gcattgagcc atatgccagt
     2101 ttatccagca tttttataaa tagactggct ttgagtggca cttgattaga taacaaagta
     2161 gatgggatgc tcatgaacta atctccattt gtgggggtaa tggcttgcga tgaaaaattg
     2221 cacctttacg ccatagtttt aaggcttgcc aatgaatacg ggcgacaact aataccgttg
     2281 tccaacctaa tttggcgagc tgttggcaaa tattgctcaa gctcaaatct tgggcttggc
     2341 ctgtgaccac tgtttttagg gtgagttgtt gattaacaaa ataattaatc gcgacgctac
     2401 gtgtattttt tttatggaca aaacgaaatt gatattcacc ttcaaccgca aaaaagggtg
     2461 aaacatgaaa gattttttcg caatttaaag tggtgtggtt atcaattact tgatgatgag
     2521 aggcagtgag taaataacaa tgccgttcgc caaaggtatt attaacctcg cacaaaatca
     2581 ctctaagctg ttgttgttgg tcatagcagt accaaaaact gacaggatta aacacatagc
     2641 ccaaaacacg aggttggctt tgtagccaga tttcgccatt agcgatttgg tctaagtttt
     2701 tgtcggctaa aatttgttta atccattgtt cggcatcttg gccattacca tgatcttgtt
     2761 cataaaaaga caataaatta aaacggttat aaccaaagcc agctcttgca agttgatgac
     2821 gcgcgctgag
//
LOCUS       DAOQMB010000301         2813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20754_length_2813_cov_15.751994, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000301 DAOQMB010000000
VERSION     DAOQMB010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20754_length_2813_cov_15.751994"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLY05_09120"
     CDS             complement(<1..874)
                     /locus_tag="PLY05_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03499.1"
                     /translation="MFKHYQGVVLSSLLFLTACNDSKEENVIVPVTTNSLAQAAAAVI
                     ASHTLFDTVITEHTNTIEEVAVEKGSASIACGTSVNAGSFLLTLKTETTYPKDGVLMN
                     HNCQEGGETFNGKLDYSCLDNNCDTGNSVATVLSWTHPAIVSFEPVVTGVWTWKLNNS
                     KQFEDEFKGEVTIKKANLSTKFAFGEGVKRVFVGKNTFDVQGKLDVSQGNSVNCVDGA
                     VSYQSTQSLTMASGSQRLTGGVMTISNSLGETGVVTFNADGSLKVSKNGVETVVPKAD
                     FESYCGLKEAYGLSE"
     gene            987..1406
                     /locus_tag="PLY05_09125"
     CDS             987..1406
                     /locus_tag="PLY05_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03500.1"
                     /translation="MSVTAEQIAQLNLLNRSCFYREKSLFVKVKVENIELTDNQCQIT
                     ISYLSPQSGKARSLNVGGNLEFFSLSSHALCLGGYIAFFLIFDEDLVQKLTVMLTDYP
                     HLDLEQRFEIGYDASLNKLKTIEYYLQYQYSSDELED"
     gene            complement(1444..2763)
                     /locus_tag="PLY05_09130"
     CDS             complement(1444..2763)
                     /locus_tag="PLY05_09130"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HNC03501.1"
                     /translation="MGKNVVVLGTQWGDEGKGKIVDLLTDNAAAVVRYQGGHNAGHTL
                     VVGGEKTVLHLIPSGILREGVLCLIGNGVVLAPDALLKEMNTLEAKGVPVRQRLRISP
                     NCPLILPYHCALDKAREAARGKSAIGTTGRGIGPAYEDKAARRGLRLGDLIRGDGFAE
                     KLAAVMEYHNFQLQHYYKADPVDFQQTLDAALAWGEQIKDLVADVSTILHDFRRAGKD
                     LMFEGAQGSLLDIDHGTYPFVTSSNTTAGGTSTGSGFGPLYLDYVLGITKAYTTRVGG
                     GPFPTELPYDAASDSGDAIGKHLGVKGHEFGATTGRQRRCGWFDAVVLRRAVEINSIS
                     GLCLTKLDVLDGLDSIKICVGYKSLKPNSVECLSSDAESFAHVEPIYEEMAGWEGTTV
                     GAKSLDDLPANARAYIKRIEEVVGCPIDIISTGPDREETIVLRHPFN"
BASE COUNT          883 a          657 c          478 g          795 t
ORIGIN      
        1 tctccgacaa gccataagcc tcttttagcc cacaatacga ctcaaaatct gcttttggca
       61 caaccgtttc caccccattt ttactcacct ttaacgaacc atccgcatta aaagtcacca
      121 caccagtttc acccaaacta ttactaatcg tcatcacacc accagtcaaa cgttggctac
      181 cacttgccat tgttaaactt tgtgtggact gataactcac cgctccatca acacaattta
      241 cagaattacc ttgcgaaaca tctaacttac cttgcacatc aaaggtattt ttgccaacaa
      301 atacgcgctt taccccctcg ccaaaagcaa attttgtact caaattagct tttttaatag
      361 ttacctcacc tttaaactca tcttcaaatt gcttactatt atttaacttc catgtccaaa
      421 ccccagttac aacaggctca aatgacacaa tcgctggatg cgtccatgac aacactgttg
      481 ctacactatt accagtgtca caattattat ccaaacagct atagtccaac ttaccattaa
      541 atgtttcacc cccctcttga cagttatgat tcatcaacac accatcttta gggtaagttg
      601 tttctgtttt gagggtcagc aaaaaactgc ctgcattaac acttgtacca caagcgatgc
      661 ttgccgaacc tttttcaaca gccacctcct ctatggtatt ggtatgttca gtaatgacag
      721 tatcaaataa ggtatggcta gcaattaccg cagcggcagc ctgagctaag gaatttgtag
      781 tgactggcac aatcacgttt tcttctttac tatcattaca agcagtcaaa aataaaagac
      841 tagacaaaac aacaccttga taatgcttaa acatcttttt tctctcacaa gtttattgat
      901 gaagcattga ttttgtcaga gtgatacgac aaaaaatgtc gtgaaaatag tgataattga
      961 tttattttta gagtggagat ttagcaatgt cagtgacagc cgaacaaatt gcacaactca
     1021 atttattaaa ccgtagttgt ttttatcgag aaaaatcttt atttgtcaaa gtgaaagttg
     1081 agaatattga attaacagat aatcagtgtc agataactat cagttattta tcgcctcaaa
     1141 gcggtaaagc tcgtagtttg aatgtaggtg gcaacttaga attttttagt cttagctcac
     1201 atgctttatg tttgggcgga tatattgctt ttttcttaat ttttgatgag gatttagtcc
     1261 aaaaactaac agtgatgctt actgactatc cacacttaga cttagaacaa cgctttgaga
     1321 taggctatga tgcgtcattg aacaaattaa aaactattga atactatttg caatatcagt
     1381 acagctcaga tgagttagag gattaatatt aaagggcaga ctcataggtc tgccccaaca
     1441 acatcaatta aacggatgtc ttaacacaat cgtttcttcg cggtctgggc ctgttgaaat
     1501 aatatcaatt gggcaaccta ccacttcttc gatacgttta atataagcac gggcattagc
     1561 tggcaaatcg tctaaagact tagcaccaac ggttgtacct tcccaacctg ccatttcttc
     1621 ataaataggc tcaacatgag caaaagattc tgcgtcagag cttaaacatt ccacactgtt
     1681 tggctttaaa gatttatagc caacacaaat tttaatgctg tccaaaccat ccaacacatc
     1741 taacttagtt aaacataagc cagaaataga gttgatttcc acagcacgac gtaaaacaac
     1801 ggcatcaaac caaccacaac gacgttgacg gcctgtggtt gcaccaaact cgtggccttt
     1861 aacgcctaaa tgtttaccaa tggcatcacc tgaatcactc gcagcgtcat aaggcaattc
     1921 ggttgggaat gggccaccac caacacgtgt ggtataagct ttggtaatac ctaaaacgta
     1981 atctaagtac aatgggccaa aaccagaacc tgtgcttgtg ccacctgccg tggtatttga
     2041 ggaagtcaca aacggataag taccgtggtc gatgtctaat aaagagcctt gtgcgccttc
     2101 aaacatcaaa tctttacctg cacgacggaa atcgtgaaga atcgttgata cgtcagcaac
     2161 gaggtcttta atctgttcgc cccaagctaa tgctgcgtct aaggtttgtt gaaaatcaac
     2221 aggatcagct ttgtagtagt gctgcaattg gaagttatga tattccatca ccgctgctaa
     2281 cttttcggca aaaccatcac cacgaatcaa atcacccaaa cgcaaaccac gacgtgcggc
     2341 tttgtcttca taagctgggc caataccacg acccgttgta ccaatcgcag atttaccacg
     2401 tgcagcttca cgcgctttgt ctaaagcaca gtgataaggc aaaattaatg ggcaatttgg
     2461 cgaaatacgc aaacgttgac gaacaggcac acctttcgct tctaaggtgt tcatttcttt
     2521 taataacgca tctggtgcta ataccacacc attgccaatt aaacacaata cgccttcacg
     2581 caaaatccca gaaggaatta agtgcaaaac tgttttttcg ccaccaacaa ctaaggtatg
     2641 acctgcgtta tggccgcctt gataacgcac gacggctgct gcattatcgg tgagcaaatc
     2701 aacgattttg cctttacctt catcacccca ttgtgtacca aggacaacaa cattcttacc
     2761 catttaagat aaccctatga gaaacaataa aataaacagg acttaggcat ttt
//
LOCUS       DAOQMB010000302         2795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_20984_length_2795_cov_6.137226, whole genome shotgun sequence.
ACCESSION   DAOQMB010000302 DAOQMB010000000
VERSION     DAOQMB010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20984_length_2795_cov_6.137226"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..493)
                     /locus_tag="PLY05_09135"
     CDS             complement(38..493)
                     /locus_tag="PLY05_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004711692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HNC03502.1"
                     /translation="MTNKSAGFTLIEVMVAVVIIGILLATAVPSYQQYVIRGNRANMQ
                     AQMLTIASALERYRAQNFRYLGATLTNTGIYGGAVFPLQGQAHYSLQLTGDNTDGTPN
                     TTWVLTAKATSNTQKQDGVIMLNSIGQRCWLAGATTTTCTLGDAAQGWN"
     gene            complement(559..>2795)
                     /locus_tag="PLY05_09140"
     CDS             complement(559..>2795)
                     /locus_tag="PLY05_09140"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HNC03503.1"
                     /translation="LGPLSSAEANMQQVASLGGGKTYKATDTGALVAGLLDVVEESMK
                     KSGTISAAGVAVNQLNRLNHLDQLYYAVFDPRPNSLRWEGNLKRYRLGNDGAIYDDTS
                     PPLGPQNAVDSTTGFFKKEAKSFWSTEVDGDIASMGGAASKLPIPNDRKMYTYTGALT
                     TQTAPVNLTPIDLTNNAFNSYAQGKTGTTGDEYLNLMKWYQGYDVSSLVDDPANATTF
                     RYRMGGALHSRPLLINYGFNGSLSTAANATNQKNYVFFSTMEGTLHAIDANTGIEKFS
                     FIPGEKLSVLKELYTNPSSALPAFGMDLTWTSYRKDNDGSGQIGTGDKVWLYGGMRMG
                     GDDYYALDVTDLNSPKLMFAIDGGKAGTKYARMGQTWSEPVITNIYLQGKPKRVMIFG
                     GGYDEKHENHNLALPFADIDKGNQVYIVDAEKGDLLWMASGNSTDAPNKLVADMKFSI
                     PSSPKALDLDSDGIADVIYIGDLGGQLFRIDLDRKASSNANIAKRVKLIAQIGQTEPS
                     VTINLAAQRRFYEPPAVATFTDSSGQRFATVAIGSGYRSRPLNTITDERFYTFFDYDV
                     TKSNLLSLADTALQPVIKHADLALLDLQSSAIKQDGIDQTKKGWYVDFSTSGEKSLAS
                     PLIYSGQLLFTSYTPSQSMSSNCSPVTGNTSLYVMCMPYGKLCAAGEGAQSPASSPNA
                     NKYKQDNIMLGLGGEPTIMRGPDEDGNDSDRIIIGTTVKEFNKDVPAPTLQPTKKWRE
                     KNPK"
BASE COUNT          838 a          615 c          456 g          886 t
ORIGIN      
        1 atgaagatat atcaggtgcg gccaaaataa actgccctta attccatcct tgagccgcat
       61 cacctaaagt acaagttgtt gttgttgctc ctgccaacca acaacgttgg cctatactat
      121 tcaacataat aaccccatct tgtttttgag tattacttgt tgcttttgct gtcaaaaccc
      181 acgttgtatt tggcgttcca tccgtattat cgcctgtcaa ttgtaatgaa taatgagctt
      241 gtccctgtag aggaaaaaca gcaccaccat aaatacccgt gttggtaagg gtagcaccca
      301 agtatctaaa attttgtgcc ctataccgtt ctagtgcgga tgcaatagtt aacatttgtg
      361 cctgcatatt agcacggtta ccacgaataa catattgttg gtaacttggc acagccgtag
      421 caagtaaaat acctattatg actacagcca ccatcacttc aattagcgta aatcctgcac
      481 tcttattagt catgattaac ctcttctgtc atattaatta acaacaagcg atccttacag
      541 gatcgcttgt tattaatact attttggatt tttttcacgc cattttttgg ttggttgtaa
      601 tgtcggagct ggaacatctt tgttaaattc tttcactgtt gtaccaataa ttattctatc
      661 tgagtcgttt ccatcttcat ctggtccacg cataattgtt ggctctcccc ctagccccaa
      721 cataatatta tcttgtttat atttattagc attaggcgat gaagctggcg attgcgcccc
      781 ctctcctgct gcacataatt tgccataagg catacacatt acatataaac ttgtattacc
      841 tgttacaggc gaacaattgc tagacattga ttgacttggc gtataacttg taaacaacaa
      901 ctgaccactg taaataagtg gagaagctaa tgatttctcg cctgatgtag aaaagtcaac
      961 ataccaacct tttttggttt gatctatacc atcttgcttg atagctgacg attgtaaatc
     1021 taataatgct aaatcagcat gttttataac tggctgcaat gccgtatctg ctaaacttaa
     1081 caaattagat ttagttacat cataatcaaa aaaggtataa aatcgttcat ctgtaatcgt
     1141 atttaatggc cgacttctat aaccactacc gattgctaca gttgcaaaac gctgaccaga
     1201 actatcagta aaagttgcga ctgctggagg ctcataaaaa cgacgttgag ccgctaaatt
     1261 aatagtaaca cttggctcag tttgaccaat ttgggcaatt aacttcacac gtttagcaat
     1321 attagcattt gaagaagcct tacgatccaa atctatacga aaaagctgcc cccccaaatc
     1381 accaatatag atcacgtcag caataccatc cgaatctaaa tcaagtgctt taggcgatga
     1441 cggaatacta aacttcatat cagcaaccaa tttattagga gcatctgtgc tatttccact
     1501 cgccatccat agtaagtcac ctttttcggc atcaactata tagacttgat tgcctttatc
     1561 tatatcggca aatggcaaag ctaaattatg attttcatgc ttttcatcat aacctccacc
     1621 aaaaatcatt actctttttg gctttccttg taagtaaata tttgtaataa ctggctcaga
     1681 ccatgtttgt cccattctgg cgtatttagt ccctgcttta cctccatcaa tcgcaaacat
     1741 taatttggga ctatttaaat ctgtaacatc taaagcatag taatcatcgc cccccatgcg
     1801 cataccacca tacaaccaaa ctttatcgcc tgtaccaatt tgtcctgagc catcattatc
     1861 tttacgatag gatgtccaag ttaaatccat accaaaggca ggtaatgcag atgagggatt
     1921 cgtatataac tcttttaaca ctgatagttt ttcaccaggt ataaagctaa atttttctat
     1981 accagtatta gcatcaattg catgaagagt tccctccata gtactaaaaa acacgtaatt
     2041 tttttggtta gttgcattag ctgcagtgct taaactacca ttaaaaccat agttaattaa
     2101 tagtggacgc gaatgaagag ctccacccat gcgataacga aatgtagttg catttgcagg
     2161 atcatcgacc aaagagctaa catcataccc ttgataccac ttcattaaat taaggtattc
     2221 atcacctgtg gtgccagttt tgccttgagc ataagagtta aaagcattat ttgttaaatc
     2281 tataggtgtt aaattaactg gtgctgtctg tgttgttaag gctcctgtat aggtgtacat
     2341 ttttcgatca ttaggaatag gtaatttgct tgctgctccc cccattgaag caatatcacc
     2401 atcaacttcg gtactccaaa aacttttagc ctctttttta aagaaacctg tggtagagtc
     2461 aactgcattt tgtgggccta aaggaggact agtatcatca taaatagcac catcgttgcc
     2521 taatctataa cgttttagat ttccttccca acgtaaacta ttaggtcttg gatcaaatac
     2581 cgcataatat aattgatcta aatggtttaa acggtttaac tggttaacag caacacctgc
     2641 cgcagaaatc gtgccgcttt ttttcatcga ctcttcaaca acatccagta agcctgcaac
     2701 caatgcacca gtatctgttg ctttataagt ttttccgcca cctaaagacg ctacttgctg
     2761 catattcgct tctgcactag acaacggacc taaaa
//
LOCUS       DAOQMB010000303         2784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21150_length_2784_cov_5.660682, whole genome shotgun sequence.
ACCESSION   DAOQMB010000303 DAOQMB010000000
VERSION     DAOQMB010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21150_length_2784_cov_5.660682"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..339
                     /locus_tag="PLY05_09145"
     CDS             16..339
                     /locus_tag="PLY05_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008306802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC03504.1"
                     /translation="MEFIETPTFTRLVQELLPDEQYHKLQVSMMEDPTSGAIIKGSGG
                     IRKLRYALPNTGKSGGVRVIYYWVSAKGIFYMLLIYPKSAKDNLTDREIAILRELVKE
                     LSNAH"
     gene            329..622
                     /gene="nadS"
                     /locus_tag="PLY05_09150"
     CDS             329..622
                     /gene="nadS"
                     /locus_tag="PLY05_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HNC03505.1"
                     /translation="MHTDLFNELTQSLKEATAIAKGQIQPSRRFVLDTPDVKAVREKT
                     GLSQAEFALRLHVSPRTLQNWEQNRRTPTGAAAALIRLLNARPDVIDDFAALG"
     gene            735..1031
                     /locus_tag="PLY05_09155"
     CDS             735..1031
                     /locus_tag="PLY05_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNC03506.1"
                     /translation="MTQQDPINIVESCGNVFLDLGFPPEEAVILAMRADLMAKLRLLI
                     DAKGWTQAEASERLKIGQSRVSDLVRGKWDKFSLDMLITLAVRSGLPCELKVAA"
     gene            1096..>2784
                     /locus_tag="PLY05_09160"
     CDS             1096..>2784
                     /locus_tag="PLY05_09160"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNC03507.1"
                     /translation="MAKVTANFISEDNIEQATIALLQNTFAYQHINAFTSNPNDLNDQ
                     TGRTDKRDVVLQQRLQAKCQQLNPQVPLATIQTAIQQFMDRRVAMSPISANIQLYHHI
                     KDGITISYQDANGTKHSNEFLTLIDFKQPQNNEFLIVSQLWIQTTGHHAKAAYRRPDL
                     ILYVNGIPLVFIELKNSNVKLKNAFDDNLTSYKNDIPQLFLYNAFCVLSNATDTKVGS
                     FTAQWEHFFNWLRVNQEKEKVDKADIEAKGTSLEYLLNGLLNPTRLLDYVENFILFQH
                     DKYKIIAQNHQFLGVNHAFDNFLNREQLDGRLGVFWHTQGSGKSFSMIFYVKKIFRKV
                     QGNFSFVVVTDRDDLDGQIYKNFISTQTITEKEAAQPKNSSHLRELLHQNTKIVFTLI
                     QKFRYDKGQVYPQLFNPKAREVIVIVDEAHRTQYETLAENMRTGLTGAHFLAFTGTPI
                     LKQKKTKQWFGDYVSEYTFQQAMEDKATLPLFYEKRVPEVLLQNDDLNEELAEILEDE
                     ALTDAQQEKLERQFSQELEVIKRDDRLDTIAQDIVSHFPQRGYLGKMTHYILSNG"
BASE COUNT          946 a          556 c          536 g          746 t
ORIGIN      
        1 aacaatgagt cgcgcatgga atttatcgaa acgccaactt ttacacgtct cgttcaagag
       61 ctgttacctg atgaacaata tcacaagtta caagtctcta tgatggaaga cccgacttct
      121 ggagcaatta taaaagggag tggtggtatt agaaaattac gctacgcatt acccaataca
      181 ggaaaaagtg gtggcgtaag agtgatttat tactgggttt ccgccaaagg aattttttat
      241 atgttgctga tttatcctaa atcggctaaa gacaacttaa ctgaccgcga aattgctatt
      301 ttgcgagaac tcgtcaagga gttatcaaat gcacactgat ttatttaacg aactgactca
      361 aagtctaaaa gaagcaacag ccatagctaa aggccaaata cagccaagtc gccgttttgt
      421 attggatacg cctgatgtta aagctgtacg tgaaaaaacg gggctttctc aggcagaatt
      481 tgctcttcgt ttgcatgtta gccctcgtac attgcaaaac tgggagcaaa atagacgtac
      541 tccaacaggg gcggcagcag cacttatacg tttattaaat gcacggcctg atgtaataga
      601 cgattttgct gcattgggtt aatcattttt taacgcacat aagtgtcagc aaatctcaag
      661 caggcagcct aaaacacgcc gcgaagatat tgaacttgcc aaaaagagat acaaggaaat
      721 tattggaggc taatatgact caacaagacc ctattaacat tgtcgaatct tgcggcaatg
      781 tatttttaga cttgggtttt ccacccgaag aagcggttat tttggctatg cgtgcagact
      841 taatggcaaa attacgtttg ttgattgatg ccaaaggatg gacacaagca gaggcatcag
      901 aacgcctaaa aataggccag tcacgagtgt ccgacttggt gcgcggtaaa tgggataaat
      961 ttagtttaga tatgttaatt accttggccg tgaggtcggg cttgccctgt gagttaaaag
     1021 ttgcagcata agacctgtta agacagaaga gaagtataac aataatcaac agcgataaat
     1081 ttgcaaggaa aacaaatggc caaagtcaca gcaaacttta ttagcgaaga caacatcgaa
     1141 caagccacca tcgccttatt acaaaatacc tttgcttatc aacatattaa cgcttttaca
     1201 tctaacccca acgacttaaa cgaccaaaca ggccgcaccg ataaacgtga tgtcgtattg
     1261 caacaacgcc tacaagccaa atgccaacaa cttaatccac aagtgccgct tgccaccata
     1321 caaaccgcta ttcaacagtt tatggacaga cgtgttgcca tgtcgcccat cagtgccaat
     1381 atacaacttt atcatcacat aaaagacggc attaccatta gctatcaaga tgcaaacggc
     1441 acaaaacaca gtaacgaatt tttaacgctg atagatttta aacaaccgca aaataacgaa
     1501 tttttaattg tgtctcaact gtggattcaa accacaggcc accacgcaaa agcagcctat
     1561 cggcggccag atttaatact gtatgttaat ggcattccac tagtatttat tgagcttaaa
     1621 aactcgaatg tcaaactcaa aaacgccttt gatgataatt taacgagcta caaaaacgat
     1681 attccgcaac tgtttttata taatgcattt tgtgtgttat ccaatgccac cgacaccaaa
     1741 gtcggcagtt ttactgccca atgggaacac ttttttaatt ggttgcgtgt taatcaagaa
     1801 aaagaaaaag tcgataaagc cgacatcgaa gccaaaggca caagccttga atatttgctc
     1861 aacggcctat taaatcccac acgcttattg gattatgtcg aaaattttat tttgtttcag
     1921 catgataaat ataaaattat tgcccaaaat catcaatttt taggggtaaa tcatgctttt
     1981 gacaactttt taaaccgtga gcaattagat ggccgtttag gtgtgttttg gcatacacaa
     2041 ggctcgggta aaagtttttc gatgattttt tatgtcaaaa aaatctttag aaaagtgcaa
     2101 ggtaatttta gttttgtggt agtgactgac cgcgatgatt tagacggaca aatttataaa
     2161 aactttattt ctactcaaac cattaccgaa aaagaagcgg cacaacccaa aaatagcagc
     2221 catttaagag agttattaca tcaaaatacc aaaatagtct ttacgctaat tcaaaaattt
     2281 agatacgaca aaggccaagt ctatccacag ctttttaatc ctaaagcgcg tgaagtgatt
     2341 gtgattgttg acgaagccca tcgcacccaa tacgaaacct tagccgaaaa tatgcgaaca
     2401 ggtttaacag gcgcgcactt tttggccttt acaggcacac cgattttaaa acagaaaaaa
     2461 accaaacaat ggtttggtga ctatgtctcg gaatatacct ttcaacaagc aatggaagac
     2521 aaagctactt tgccgttatt ttatgaaaaa cgtgttcccg aagtattact gcaaaatgac
     2581 gacttaaacg aagaattagc cgaaattttg gaagatgaag cactaacaga cgcacaacaa
     2641 gaaaagcttg aacgtcaatt ttcgcaagag ttagaagtga ttaaacgaga tgaccgttta
     2701 gacaccattg ctcaagatat agtgagtcat tttccccaac gtggttattt gggtaaaatg
     2761 actcactata tcttgagcaa tggt
//
LOCUS       DAOQMB010000304         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21231_length_2779_cov_17.170338, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000304 DAOQMB010000000
VERSION     DAOQMB010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21231_length_2779_cov_17.170338"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLY05_09165"
     CDS             complement(<1..383)
                     /locus_tag="PLY05_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 associated
                     domain-containing protein"
                     /protein_id="HNC03508.1"
                     /translation="MAEITVKQLAQIVNRSVETLLVQMKDAGLRTRGADDFVSDTEKQ
                     QLVAFLKRSHGDTSEDKQITLKSKTKTTIKTPISGGKAKTVNVEVRKSRTFVKPTDAD
                     LAQDIVARKAAEEAAHKAAEEAARKA"
     gene            complement(491..1960)
                     /gene="nusA"
                     /locus_tag="PLY05_09170"
     CDS             complement(491..1960)
                     /gene="nusA"
                     /locus_tag="PLY05_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNC03509.1"
                     /translation="MSKEILLVVESVSNEKGIAREAIFQALEQALVAATKKKYDIDEM
                     DVRVSINRKTGDYDTFRRWTVVADHDHEMPACQDAISDVDTSKWQIGDVREEQIPSIE
                     FGRIAAQTAKQVIVQKVREAERALVVDAYRQRVGELVSGEVKKITRDGVIVDLGNNAE
                     GYLAREQMMPKENFRVKERVNSILYAVNSEGRGAQLLLSRTCPEMLISLFQKEVPEIG
                     EEIIEVKGAARDPGIRAKIAVKTNDHRIDPVGACVGMRGTRVQAVTAELGGERVDIVL
                     WDDNPVQFVINALQPAEVVSIVLDEDLHAMDVAVDSDQLAQAIGRAGQNVRLASELTG
                     WRLNVMTIEEANAKQASESQQFVDAFVRDLEVDEHIATILVSEGFTTLEEVAYVPIEE
                     MLAIVDFTEDLVNNMRQKAKDVLLTQALVSQEKAEPAHDLLAMEGMTRPLAYALAARG
                     VITMEDLAEQAIDDIADIEGLDAEQAAQLIMKAREPWFQ"
     gene            complement(1988..2449)
                     /gene="rimP"
                     /locus_tag="PLY05_09175"
     CDS             complement(1988..2449)
                     /gene="rimP"
                     /locus_tag="PLY05_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HNC03510.1"
                     /translation="MAVSQKVQTLITMLQPAVEACGFELWGLEFFPQGKRSVLRIYID
                     GAEGVTVEGCSQVSHQVSGILDVEDPISGEYLLEVSSPGWDRPLFTLPHYQRFVGSEV
                     NLRLVAPINARRRYKGVIQAVIDNTIELAFEGQMIRIPFAQIDKANVVPSV"
BASE COUNT          806 a          695 c          459 g          819 t
ORIGIN      
        1 gccttacgtg ctgcctcttc agcggcttta tgtgctgctt cttctgctgc tttacgtgca
       61 acaatatctt gcgctaaatc agcatctgtc ggtttgacaa aagtacgtga tttacgcact
      121 tcaacattaa ccgtttttgc tttaccacca gaaatagggg ttttaatcgt ggtcttagtt
      181 ttacttttca gcgtaatttg cttatcttct gaggtatcac catgactacg ctttaaaaac
      241 gcaaccagtt gctgcttttc ggtatcagag acaaaatcat ccgcaccacg cgttcttaac
      301 cctgcatctt tcatttgaac taacaaggtt tctacagaac gatttactat ttgtgctagt
      361 tgtttgacag ttatttcagc catgtggcat gtcactccaa aacgattcgg gaactcacac
      421 aaacctaaac gaggtgcgtg agtttatacc tgaggactaa gactttttaa gtctgtttta
      481 gtcctatgat ttattgaaac cacggttcac gggctttcat aattaattgg gctgcttgtt
      541 ctgcatccaa gccttcaata tctgcgatat catcaatggc ttgttcagct aaatcttcca
      601 tagtaatcac accacgagct gccaaagcat aagcaagtgg acgagtcatc ccttccattg
      661 ctaataaatc atgggcaggt tcagcttttt cttgcgagac aagtgcctgt gttaacaaaa
      721 cgtcttttgc tttttgacgc atgttattga ctaaatcttc agtaaaatca acgattgcca
      781 gcatttcctc aatcggtacg taagcaactt cttcgagtgt ggtaaaacct tcactcacca
      841 aaatagttgc aatatgttca tcaacttcta aatcacgaac aaaagcatca acaaattgtt
      901 gtgattcaga agcttgtttg gcattagctt cctcaatagt catgacgttt aaacgccaac
      961 ctgttaattc cgaagctaaa cggacatttt gtcctgcacg accaatggct tgcgctaatt
     1021 gatcgctgtc tacagccaca tccatagcgt gtaaatcttc atctaaaaca atagaaacca
     1081 cttctgctgg ttgtaaggcg ttaatcacaa actgaactgg attatcatcc cacaacacaa
     1141 tatcaacccg ctcaccaccc aactccgcag tcactgcttg tacacgagta ccacgcatac
     1201 ccacacatgc acctacagga tcaatacgat ggtcattagt tttcacggct attttagcac
     1261 gaataccagg atcacgagcg gctcctttga cttcaataat ttcttcgcca atttctggca
     1321 cttctttttg aaacaaagag atcaacattt caggacaggt tcgtgacaat aacaattgtg
     1381 caccacgccc ttcactgttc accgcataca aaatactatt cacacgttct ttgacacgaa
     1441 aattttcttt cggcatcatt tgttcacgtg ctaaatagcc ttctgcgttg ttacccaagt
     1501 caacaatcac cccatcacgg gtaatttttt tcacttcacc agaaactaat tcacccacac
     1561 gttgacgata agcatctacc accaaagcac gctccgcttc gcgtactttt tgtacaatca
     1621 cttgtttagc ggtttgtgca gcaatacgac caaattcaat cgaaggaatt tgctcctcgc
     1681 gaacatcacc aatttgccat ttgctggtat ctacatcact aatagcatcc tgacaagctg
     1741 gcatttcatg atcatggtct gcaaccaccg tccaacgacg aaatgtatcg taatcacccg
     1801 ttttacgatt aatgctgaca cgcacatcca tctcatcaat atcatatttc tttttggttg
     1861 cagcaactaa agcttgctct aatgcctgaa aaattgcctc acgtgcaata cctttttcat
     1921 tactgacgga ctcaaccacc aataaaatct ctttgctcat actactgccc tgatataaca
     1981 aaattattta gacactagga accacattgg ctttatcaat ttgtgcgaag ggaatacgaa
     2041 tcatctgccc ttcaaatgcc agctcaatgg tattatcaat cacagcttga ataacgcctt
     2101 tataacgacg acgcgcatta attggcgcaa ccaagcgtaa attcacttca ctaccgacaa
     2161 aacgctgata atgcggcaag gtaaacaatg gacgatccca acccggtgat gatacttcta
     2221 acaaatactc acccgaaatt gggtcttcaa catccaaaat accgcttact tgatgactaa
     2281 cctgtgaaca cccttcaaca gtgactcctt ctgcgccatc aatataaata cgtaacaccg
     2341 agcgtttgcc ctgtggaaaa aactcaagtc cccacaattc aaagccacaa gcctcaacag
     2401 ctggctgcaa cattgtaatg agcgtttgaa ctttttgcga aaccgccatg tacttctctt
     2461 cttactttag aacttacact gtcattgttt atttgcttac atatcaatcg tctttaacgc
     2521 tgttatgctt acaaaacacc taagccctgt tcttatcaaa aataaaaaat gggcataacg
     2581 cccactctca taaagtctat tacatattag cagcatagac ttttttctga tctttattgt
     2641 aaaaataaaa atcagtttct ttcaggtgca aaaaagccca catatagtgg gcttctttga
     2701 cataaaaact tggtagcggg ggctggattt gaaccaacga ccttcgggtt atgagcccga
     2761 cgagctacca gactgctcc
//
LOCUS       DAOQMB010000305         2768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21396_length_2768_cov_15.070770, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000305 DAOQMB010000000
VERSION     DAOQMB010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21396_length_2768_cov_15.070770"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLY05_09180"
     CDS             complement(<1..428)
                     /locus_tag="PLY05_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03511.1"
                     /translation="MLTHLAIKQFTLVENLELDIAQGFTVITGETGAGKSILLDALGL
                     CLGDKADGQAVRHGADKAELSALFDVSQLPQAQAWLTERDLAEDECCLRRVVYKEGRS
                     KAWVNGSVVTLAELRSLGELLINIHSQHAHQSLLKKETHR"
     gene            585..>1111
                     /gene="grpE"
                     /locus_tag="PLY05_09185"
     CDS             585..>1111
                     /gene="grpE"
                     /locus_tag="PLY05_09185"
                     /inference="COORDINATES: protein motif:HMM:NF010748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNC03512.1"
                     /translation="MTEQQKDVDNAQINAEIEQELNEYLEVTETDVEALTAKVTQLEA
                     QLKDTQLRAQAEIRNIQARAERDVTNAHKFGVEKFANSLLDVVDNLERAVAAAPQNEA
                     NKNLVEGLQLTHKAFLDALKKFGVEAVDPAGQAFSAELHQAVATQPASASNPANTVVT
                     VYQKGYTLSGRLMRPA"
     assembly_gap    1112..1211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1351..>2768
                     /gene="dnaK"
                     /locus_tag="PLY05_09190"
     CDS             1351..>2768
                     /gene="dnaK"
                     /locus_tag="PLY05_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNC03513.1"
                     /translation="MGKIIGIDLGTTNSCIAIMEGDKAKVIENAEGARTTPSIVAYTE
                     NEVLVGAGAKRQAVTNPKNTLFAVKRLIGRKFKDDVVQKDIGMVPYEIVAADNGDAWV
                     SARGNRMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDSQRQATKDAGRIA
                     GLDVKRIINEPTAAALAFGMDKKEGDRKIAVYDLGGGTFDISIIDIADVDGEQSFEVL
                     ATNGDTFLGGEDFDLRLIDYLADEFKKEQSINLKGDPLAMQRLKEAAEKAKIELSSSQ
                     QTEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVARTIEPCKIALKDAGLSVGQIG
                     DVILVGGQTRMPLVQQKVKEFFGKEPRKDVNPDEAVAVGAAIQGAVLSGGVTDVLLLD
                     VTPLTLGIETMGGVMTALIEKNTTIPTKKSQVFSTADDNQTAVTIQVYQGERKMAQQN
                     KLLGRFDLSDIPPAPRGMPQIEV"
BASE COUNT          849 a          541 c          569 g          709 t
ORIGIN      
        1 tgtcgatggg tttctttttt gagtaggctt tgatgagcat gttggctatg aatattaatt
       61 aacagttcac ccaaagagcg caattcagcc agtgtgacta cactaccatt aacccatgct
      121 ttcgagcgtc cttctttata aaccacacgc cgcaaacaac attcgtcttc ggctaaatca
      181 cgctctgtga gccatgcttg tgcttgaggc aattgactga catcaaataa agcactgagt
      241 tccgctttat ctgcgccatg acgcacggct tggccatcgg ctttatcgcc taaacataag
      301 cccaaagcat ccaataaaat agacttacca gcacctgttt cccccgtaat caccgtaaat
      361 ccttgggcta tatctaattc taagttttca acaagggtaa attgtttaat ggctaaatgc
      421 gtcaacatga tgatcagtat cagtaaaaaa gtttgcgtga taataccctg actttttatc
      481 taagcaatag ccgaagcact tgaataattg gtgactaacc ccattaaatt tgcagatttt
      541 attggctaaa gcccaaaagt taatttttga ttggggagaa aatcatgacc gaacagcaaa
      601 aagatgttga taacgcacaa attaatgccg aaatcgaaca agagttaaat gaatatttag
      661 aagtcaccga gactgacgtt gaagcgttaa ccgccaaagt cacacaatta gaagcacaac
      721 tcaaagatac tcaattacgt gctcaagcag agattcgtaa tattcaagcg cgtgctgaac
      781 gtgatgtgac caatgctcat aaatttggtg ttgaaaaatt tgccaatagc ttattagacg
      841 tggtggataa cttagagcgt gctgtagccg cagcaccaca aaacgaagct aacaaaaact
      901 tggttgaagg tttgcaatta acgcacaaag cctttttaga tgcgttaaag aaatttggtg
      961 ttgaagcagt tgatccagca ggtcaagcgt ttagtgctga gttacatcaa gcggttgcca
     1021 ctcaacctgc gagtgcaagc aatccagcca atacggtcgt gacggtttat caaaaaggtt
     1081 acaccttgtc tggtcgttta atgcgtccag cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn naaaaaaacc ttgaaaaatt taaggtaatc accatatcag aatcatgtaa
     1261 acgatgtgat gaaaaagcaa gcatcttcaa acaaataatc taacgaaggc tcaataagtc
     1321 cttcgattaa caccgcttta ggagcaaaaa atgggcaaaa ttattggtat cgacttaggc
     1381 acaaccaact cttgtatcgc tattatggaa ggcgataaag ccaaagttat cgaaaatgcc
     1441 gaaggcgcac gtactacacc ttctatcgtg gcttataccg aaaacgaagt tttagtcggt
     1501 gcaggcgcaa aacgtcaagc agtaacgaat cctaaaaaca ccttattcgc ggttaagcgt
     1561 ttaattggtc gtaaatttaa agatgatgtt gtgcaaaaag acatcggcat ggtgccttac
     1621 gaaatcgttg ctgctgacaa tggcgatgct tgggtatctg ctcgcggtaa ccgcatggca
     1681 cctccacaaa tctctgctga agtcttgaag aaaatgaaaa agacagcaga agattattta
     1741 ggtgagccag tcacagaagc cgttattact gttcctgctt actttaacga cagccaacgt
     1801 caagcaacta aagatgcagg ccgtattgct ggtttagatg ttaaacgtat cattaacgaa
     1861 ccaacagcgg ctgctttagc ctttggtatg gacaaaaaag aaggcgaccg taaaatcgct
     1921 gtctatgact taggtggtgg tacattcgat atttctatta tcgacatcgc tgatgttgat
     1981 ggcgaacagt cttttgaagt attagccact aatggtgata ctttcttagg tggtgaagat
     2041 tttgacttac gcttaattga ttatttagca gatgagttca aaaaagaaca aagcatcaac
     2101 cttaaaggtg atcctttagc gatgcagcgt ttaaaagaag cggctgaaaa agccaagatt
     2161 gaattatctt ccagtcaaca aaccgaagtt aacttacctt acatcactgc tgatgccaca
     2221 ggtccaaaac accttaacat caaagtgaca cgtgctaagt tagaatcttt ggttgaagat
     2281 ttagtcgcac gcaccatcga gccatgtaaa attgccttaa aagatgctgg tttaagtgtt
     2341 ggccaaattg gtgacgtgat tttggtgggt ggtcaaactc gtatgccttt agttcaacaa
     2401 aaggttaaag agttctttgg taaagagcca cgtaaagacg ttaacccaga cgaagcggtt
     2461 gccgtgggtg cagcgattca aggtgcggtg ttatcgggtg gtgtaacaga cgtattgttg
     2521 ttagacgtaa cgccattaac ccttggtatt gaaacgatgg gtggtgtgat gactgcctta
     2581 atcgagaaaa acaccacgat tccaaccaaa aaatcacaag tgttctcgac tgctgacgac
     2641 aaccaaactg ctgtgaccat tcaagtttac caaggtgagc gtaaaatggc acaacaaaac
     2701 aaattgcttg gccgttttga tttaagtgat attccaccag caccacgtgg tatgccacaa
     2761 atcgaagt
//
LOCUS       DAOQMB010000306         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21410_length_2767_cov_15.716445, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000306 DAOQMB010000000
VERSION     DAOQMB010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21410_length_2767_cov_15.716445"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="PLY05_09195"
     CDS             <1..1110
                     /locus_tag="PLY05_09195"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HNC03514.1"
                     /translation="EIIAGETRIAATPDTVKKLVAAGHKVVVQAGAGVKASLPDANFS
                     AVGASIVDATTAYASDIILKVQAPTAEEIASIKDGATIIAMFDPYRNANLQAFADKQL
                     NCFALELLPRTLSRAQNMDVLSSQSNLAGYKAVIMAADEYQRPFPMFMTSAGTVKPAR
                     VVIMGVGVAGLQAIATAKRLGAVVEATDLRPTAKDQVESLGGKWLDVPMSDEEKENAK
                     KAAASGYAWMPGPQYIADQAVIVDKAVSQADIVITTALIPGRNAPRLIKAETVAKMKA
                     GSVILDMAVETGGNVEGSVLNQITTTDNGVKIIGIPNIPAQLAAQSSALYANNVYNFL
                     ATLFNKEGQMVVNLEDEIQKALLITQAGQLIFGKK"
     gene            1122..1460
                     /locus_tag="PLY05_09200"
     CDS             1122..1460
                     /locus_tag="PLY05_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HNC03515.1"
                     /translation="MNEVAQVAVQAAEHAASVPFVSVFTIFVLAIFVGYYVVWGVTPA
                     LHTPLMAVTNALSGIIVVGAMLQAVTIDGSDITATSLLGAFAVFLASINIFGGFAVTE
                     RMLDMFKKKK"
     gene            1473..>2767
                     /locus_tag="PLY05_09205"
     CDS             1473..>2767
                     /locus_tag="PLY05_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HNC03516.1"
                     /translation="MQGFAQYADWFYLVGAILFILTLRGLSGPKTAIMGNRYGMMGMA
                     LAVLTTFFVAPSPVLGLIIGAMVAGAAIGIYRARTVPMTQMPETVAMMHSLVGLAAVA
                     IAIAAVLHTGVKHDGIHRFELFVGCFVGAITFTASVFAYGKLAAKKWAKTVKGSWVKP
                     TQAALFIAMLGFGTNYFVSEDLTAFYLMTAFALAFGYIWIAPIGGGDMPVVVSLLNSF
                     SGWAAAGIGFTLNNSMLIIAGSLVGSSGAILSYVMCKAMNRSLMNVLFGGFGATATDT
                     ADSGEVKQKNYKSGSPEDAGFLMSNASSVIIVPGYGMAQGRAQNAVKELYNTLKAEGV
                     NIRFAIHPVAGRMPGHMNVLLAEADVPYEDILEMDEINSDFAGTDVVLIIGANDVVNP
                     AAKDDKSSPIYGMPILEAYKAQTIMVIKRSMSTGYAGLD"
BASE COUNT          715 a          536 c          682 g          834 t
ORIGIN      
        1 gaaatcatcg ctggcgaaac acgcattgca gctacgcctg ataccgtcaa aaaattggtg
       61 gcggcaggtc ataaagtagt tgtgcaagcg ggtgctggtg taaaggccag tctgccagat
      121 gccaatttca gcgcagtagg cgcgtctatt gttgatgcta caacggctta tgccagtgac
      181 atcattctta aggtacaagc accaaccgca gaagaaattg cgtcgattaa ggacggggca
      241 acgattattg ccatgttcga cccttatcgt aatgctaatt tgcaagcctt tgctgataag
      301 caattaaatt gctttgcctt agagttatta ccacgtactt tgtctcgtgc gcaaaatatg
      361 gacgttttat cctcccagtc taacttggca ggttataaag cggtcattat ggcagctgac
      421 gaatatcaac gtccattccc aatgtttatg acctccgcag gtacggtaaa accagcgcgt
      481 gtggttatta tgggtgtggg tgtggcaggt ttacaagcga ttgcaactgc taaacgtttg
      541 ggtgcagtgg tagaagcaac agacttacgt ccaaccgcta aagaccaagt tgagtcttta
      601 ggtggcaagt ggcttgatgt gccaatgtct gatgaagaga aagaaaacgc taaaaaagcc
      661 gctgcgagtg gttatgcgtg gatgcccggc ccacaataca ttgctgacca agcggtgatt
      721 gttgacaaag ctgtttctca agcagacatc gtgattacga ctgccttgat tccaggccgt
      781 aatgctcctc gtttaattaa agccgaaact gtggccaaaa tgaaagctgg ctcagtgatt
      841 ttagatatgg cagttgaaac aggtggcaac gtcgaaggtt ctgtgttgaa tcaaattacc
      901 acgaccgaca acggcgtaaa aatcattggt atcccgaata ttcctgcgca attagccgca
      961 caatcttcgg ctttatatgc caacaacgtc tataacttcc tcgcgacttt attcaacaaa
     1021 gaaggtcaaa tggtagttaa cctcgaagac gaaatccaaa aagccttgtt aattactcaa
     1081 gcaggccaat taatttttgg taagaagtga ggagctaagt catgaatgaa gttgctcaag
     1141 ttgccgttca agcggccgaa cacgcagcca gcgtcccttt tgttagtgtc ttcacgattt
     1201 ttgtgttggc aatttttgtc ggttattacg ttgtttgggg cgtaacccca gccttgcata
     1261 caccattaat ggcggtaact aatgctttgt caggcattat tgttgtcggt gcgatgttac
     1321 aagcagtgac cattgatggc agtgacatca ctgcaaccag tttattaggc gcatttgccg
     1381 tgttcttggc cagtatcaac atctttggtg gttttgccgt caccgaacgt atgcttgata
     1441 tgttcaagaa aaagaagtaa gaggtgacga cgatgcaagg ttttgctcaa tacgcggatt
     1501 ggttttacct agttggtgct atcctcttta ttttgacatt acgtggttta tctggtccaa
     1561 aaacagcgat tatgggtaac cgttacggga tgatgggtat ggcattagcc gtattaacca
     1621 cattcttcgt tgcgccaagt cctgtgttag gtttaattat tggtgcaatg gttgcgggtg
     1681 ctgctatcgg tatttatcgt gcgcgtactg tacccatgac ccaaatgcca gaaactgtag
     1741 caatgatgca ctctttagtg ggtttagctg cggtagcgat tgcgattgcg gcggtattac
     1801 acacaggtgt aaaacacgat gggattcatc gttttgaatt gtttgtcggt tgttttgttg
     1861 gtgcgattac ttttacggct tcggtgtttg cttatggcaa attggcagct aaaaagtggg
     1921 ctaaaactgt taaaggtagc tgggttaagc caacacaggc cgctttgttt attgcgatgt
     1981 taggttttgg tacaaactat tttgttagtg aagacttaac agctttctat ttaatgaccg
     2041 cttttgcttt agcctttggt tatatctgga ttgcaccaat tggtggcggt gatatgccag
     2101 tggttgtgtc tttattaaac tctttctctg gttgggcagc ggctggtatt ggttttacct
     2161 taaataactc gatgttgatt attgcgggtt ctttagtggg ttcttctggt gcaattttgt
     2221 cgtatgtgat gtgtaaagcc atgaaccgtt cattaatgaa cgtattgttt ggtggttttg
     2281 gtgcaacagc aactgatacg gctgatagtg gcgaagttaa gcaaaagaac tacaaatctg
     2341 gttctccaga agatgcaggt ttcttaatgt ctaatgctag cagcgttatc attgttccag
     2401 gttatggtat ggctcaaggc cgcgcacaaa atgcggttaa agagttatat aacaccttaa
     2461 aagcagaagg cgtgaatatt cgttttgcga ttcacccagt tgcgggtcgt atgccaggtc
     2521 acatgaacgt attgttggca gaagctgacg taccttacga agacatctta gaaatggacg
     2581 aaattaactc cgatttcgca ggcactgatg tagtgttaat tatcggtgct aatgacgttg
     2641 ttaaccctgc tgctaaagat gataaaagct ctccaattta tggtatgcct attttggaag
     2701 cttacaaagc tcaaaccatt atggtgatta agcgttctat gtctacaggt tatgcaggct
     2761 tagataa
//
LOCUS       DAOQMB010000307         2767 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21412_length_2767_cov_13.431785, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000307 DAOQMB010000000
VERSION     DAOQMB010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21412_length_2767_cov_13.431785"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /gene="serA"
                     /locus_tag="PLY05_09210"
     CDS             complement(<1..1142)
                     /gene="serA"
                     /locus_tag="PLY05_09210"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HNC03517.1"
                     /translation="MSQLSLDKAKIRFLLLEGVHKNALDVLSNNGYTNIEYLRGALDE
                     DALIEKIKDVHFLGIRSRTNVTEKVLAAANKLISIGCFCIGTNQVNLKAAMEKGIPVF
                     NAPYSNTRSVAELVLGETILLLRGIPEKNALVHRGGWSKSAEGSFEARGKVLGIVGYG
                     SIGTQLSVLAESLGMQVIYFDVVTKLPLGNARQVGSMSELLAQADVVSLHVPELASTK
                     NMMGAEQFAQMKKGSIFINAARGTCVVIDDLAAALESGHIAGAAIDVFPKEPKANDEE
                     FLSPLRKFDNVILTPHIGGSTLEAQANIGLEVAEKFARYSDTGTTLSAVNFPEVAIPL
                     QAGKHRLLHIHNNVPGVLSAINRVFAENNINISAQSLMTNEAIGYLV"
     assembly_gap    1181..1280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1371..>2767
                     /locus_tag="PLY05_09215"
     CDS             1371..>2767
                     /locus_tag="PLY05_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC03518.1"
                     /translation="MLNPTIITQLQAIVGETRVKTDADSLKTWGCDWTKHYTPSASAI
                     VFPATTEEVQAIVKLANQENIVLVPSGGRTGLSAGAVAANNEVVISFDRMNKIVQFYP
                     ADRMVKVQAGVVTEQLQQFADEQGLYYPVDFASAGSSQIGGNIGTNAGGIKVIKYGMT
                     RAWVLGLTVVTGKGDILHLNKGMMKNATGYDLRHLFIGAEGTLGFVTEAEIKLERKPR
                     DLRVLVLGTPDFDSVMKILSAFQAKLDLTAFEFFSDLAVQCVTASGDVQRAFPEATPF
                     YALLEFEALSPAIEEQAMQVFEQCMEEGWVINGVMSQNETQAQNLWRLREDISETIAK
                     YTPYKNDISVLITHVPAFIADIDAIVQENYPDFDIVWFGHIGDGNLHLNILKPANLSK
                     DEFFGKCQVVNKKVFETVQKYNGSISAEHGVGMTKKPYLDYSRSPEEIAYLQAIKLAF
                     DPNNIINRGKIFDVK"
BASE COUNT          789 a          525 c          592 g          761 t
ORIGIN      
        1 accaaataac caatcgcttc gttagtcatt aagctttgag cagaaatgtt gatgttgttt
       61 tcggcaaata cacggttaat tgcagataac acacctggta cgttgttgtg gatgtgcaat
      121 aagcggtgtt tgccagcttg taaaggaatg gccacttctg ggaagtttac tgctgataag
      181 gttgtgcctg tatcagaata acgagcaaat ttctcagcca cttctaaacc gatattggct
      241 tgggcttcta aggtagaacc gccaatgtgt ggtgttaaaa tgacgttatc aaatttgcgt
      301 aatggcgata aaaattcttc gtcattcgct ttgggctctt tagggaacac gtcaatcgcc
      361 gcacctgcaa tatgaccact ttctaaagca gccgctaagt catcaatgac tacacaagta
      421 ccacgagccg cattaatgaa gatgctacct tttttcattt gggcgaattg ttcagcaccc
      481 atcatgtttt tggtagaggc taattcgggt acatgtaaag aaacgacatc agcttgggct
      541 aataattcgc tcatagaacc gacttgacga gcattaccca aaggtaattt ggtgacaacg
      601 tcaaaataaa tcacttgcat acccaaagat tcagctaata ccgaaagctg agtaccaatg
      661 ctgccgtaac caacaatacc taaaactttg ccacgtgctt caaaagagcc ttcagcagat
      721 ttagaccaac caccacgatg tactaaagcg ttcttttcag gaataccgcg taataacaaa
      781 atggtttcgc ctaagactaa ctcagcaacc gagcgagtat tagaataagg cgcgttaaat
      841 acgggaatgc ctttctccat ggcggctttg aggttaactt ggttagtacc aatacagaag
      901 cagccaatag aaatcagttt attagccgca gctaatactt tttcggtgac attggtgcga
      961 gagcgaatac ctaaaaagtg gacatctttg attttttcga tgagcgcatc ttcatcaaga
     1021 gcaccacgta agtattcaat attggtatag ccgttattac ttaatacatc gagggcgttt
     1081 ttgtgtacac cttctaacaa caaaaagcga attttggctt tatctaaaga caattggctc
     1141 atgacaattc ctgaaggccg tggagaaaga cgatggactt nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn aagtcctaaa aataagtcga atatgataac ataagccgat
     1321 actgtatagc caataggcaa tacgctgtaa tgctttttag gaatagacac atgctaaatc
     1381 caaccattat tacccaactc caagccattg ttggcgaaac tcgtgttaaa accgatgctg
     1441 acagcctaaa aacatggggc tgtgattgga caaaacacta caccccctct gcgagtgcca
     1501 ttgtttttcc tgcaacgacc gaagaagtac aagcgattgt caaattggcc aatcaggaaa
     1561 atattgtctt agtcccatca ggtggtcgca caggtttgtc cgcaggtgct gtcgcggcca
     1621 ataatgaggt ggtgattagc tttgaccgta tgaacaaaat tgttcagttt tatcctgccg
     1681 accgtatggt caaagtacaa gcaggggtgg tgactgagca attacaacaa tttgcggatg
     1741 agcaaggttt gtattacccc gtagattttg cctcggcagg ttctagccaa attggtggca
     1801 atatcggcac taacgcgggt ggtattaaag ttattaaata cggcatgact cgtgcttggg
     1861 tcttgggttt aaccgtggtc acaggtaaag gcgatatttt acacctcaat aaaggcatga
     1921 tgaaaaatgc cacaggttat gacttacgcc atttgtttat tggcgcagaa ggcacgttgg
     1981 gttttgttac cgaagcagaa attaaactcg aacgtaaacc gcgtgatttg cgtgtgttgg
     2041 tgttgggtac acctgacttt gactcggtca tgaaaatttt atcggctttt caagctaagt
     2101 tagatttaac cgcgtttgag tttttctccg atttagcggt gcaatgtgtg actgccagtg
     2161 gcgatgtaca gcgtgccttt cctgaagcaa cgccgtttta tgcgttgtta gaatttgaag
     2221 ccttatcgcc tgctattgaa gaacaagcca tgcaagtgtt tgagcaatgt atggaagagg
     2281 gttgggtaat taatggggtg atgagtcaaa acgaaaccca agcccaaaac ttgtggcgtt
     2341 tgcgtgaaga tatttctgaa accattgcca aatatacacc gtacaaaaat gatatttcgg
     2401 tattgattac tcacgttcct gcgtttattg ccgatattga tgccattgtg caagaaaact
     2461 atcctgactt tgatattgtc tggtttggtc atattggcga tggtaatttg cacttgaata
     2521 ttttaaaacc tgctaacttg agcaaagatg agttttttgg caaatgccaa gtggttaata
     2581 aaaaagtgtt tgaaacagtg caaaaataca atggttctat ttcggcagaa catggtgtcg
     2641 gtatgaccaa aaagccgtat ttagactata gccgtagccc cgaagaaatt gcttatttac
     2701 aagcgattaa attggcattt gaccccaata acattattaa tcgcggtaag atttttgatg
     2761 tgaagta
//
LOCUS       DAOQMB010000308         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21713_length_2745_cov_8.411524, whole genome shotgun sequence.
ACCESSION   DAOQMB010000308 DAOQMB010000000
VERSION     DAOQMB010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21713_length_2745_cov_8.411524"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="PLY05_09220"
     CDS             <1..2083
                     /locus_tag="PLY05_09220"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNC03519.1"
                     /translation="SAAFGGLVFFSGVFAKQASAPSPVEPNKEEVKAARSTVMMLKHL
                     HYEHKKLNDALSSQILDRYLKDLDSTRAYFLASDIAEFEEYRYLLDDDLNRGDLTHGF
                     AIYARYQQRFNQRLDYVLKELKKGSKNFDFSVDDSLEIKRENVPWAKEAQELDTLWYK
                     RLKSAVLSLKLSGKKDEEIFKLLTKRYETQRNNMNRNKADDVFQTFMNAFTETYDPHT
                     EYFSPRTSENFNINMSLSLEGIGAVLQAEDDYTKIVRLVPAGPADKSKQLKPNDRIVA
                     VAQGDGEFVDVVGWRVDEVVDLIRGPKNSTVRLQVLPANAVDEHQTKVISIVRQTIKL
                     EEQAAQKRVITINRQGKDYKIGVIKLPTFYADFAAMQAGDPNYRSTTRDVAKLIEELK
                     AEQIDGMVLDLRNNGGGSLSEANSLIGLFIETGPTVQVKNANLEVELLGDSNPAVAYD
                     GPLVVMTNRLSASAAEIVAGAIQDYGRGLVVGSTTYGKGTVQALRELNHGQLKLTEAK
                     FYRISGASTQHKGVEADIHFPNIFQGGDIGESYLENALPWDTISPARYLPYSGFSRQL
                     PILKLKSQQRQTSNPDFKYLNAQITLAESIKKETTISLNEAKRKKEQEELDNKRLAIE
                     NERRAAKGQELLKTWREAEAANGDDKEDELADNNKKEKPEDEAFVTEAAEVLLDATLT
                     ENPKETIAKGR"
     gene            complement(2080..2433)
                     /locus_tag="PLY05_09225"
     CDS             complement(2080..2433)
                     /locus_tag="PLY05_09225"
                     /inference="COORDINATES: protein motif:HMM:NF017868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HNC03520.1"
                     /translation="MENTYLFVYGTLRQQSTHPMGLWLAQHAEFIGHAKVRGHLYQVS
                     YYPAVVDGDSWIEGDVYACAADLWPILDEFEEVNTQNPEYERRLTPVLLTTGQWLTAW
                     VYWYLQPTDHLRRVV"
     gene            2432..>2745
                     /locus_tag="PLY05_09230"
     CDS             2432..>2745
                     /locus_tag="PLY05_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNC03521.1"
                     /translation="MSLPLVLASTSIFRRELLTRLTSDFSQDSPNVDESPLANESAPQ
                     LAQRLAILKAQALTTQYPRHLIIGSDQVASFDNKLIGKPHTHQNAIQQLLTFSGQRVD
                     FYT"
BASE COUNT          876 a          537 c          547 g          785 t
ORIGIN      
        1 gtcagccgct tttggtggct tagtcttttt ttcaggtgtg tttgccaaac aagcctccgc
       61 tccctcacct gttgaaccaa acaaggaaga agttaaagca gcgcgttcca cggtgatgat
      121 gcttaaacat ttgcattatg aacacaaaaa actcaatgac gcattatcga gtcaaatttt
      181 agaccgttat ctaaaagatt tagactcgac acgtgcctat tttttggcca gtgatattgc
      241 tgagtttgaa gaatatcgtt atttgctaga tgatgactta aatcgtggcg atttaacaca
      301 tggttttgcc atttatgcac gttatcaaca acgttttaat caacgtttag attatgtgtt
      361 aaaagaactc aaaaaaggca gcaaaaactt tgactttagt gttgatgata gtttagaaat
      421 caaacgtgaa aatgtgcctt gggcaaaaga agcacaagag ttagatacct tatggtacaa
      481 acggctaaaa tcagcggtgt taagccttaa actgtctggc aaaaaagatg aagaaatctt
      541 taaactatta accaaacgtt acgaaaccca acgtaacaac atgaatcgta ataaagcgga
      601 tgatgtattt caaactttta tgaacgcatt taccgaaact tacgatcctc ataccgaata
      661 cttttcacca cgcacttccg aaaacttcaa tattaatatg agtttatctt tggaaggcat
      721 tggcgcagta ttacaggccg aagacgacta tactaaaatt gtgcgtttag tacctgctgg
      781 ccctgcggat aaatccaaac aactcaaacc taatgatcgt attgtcgctg ttgcccaagg
      841 tgatggtgaa tttgttgatg tggttggttg gcgtgttgat gaggtggttg atttaattcg
      901 tggtcctaaa aatagcaccg tacgtttaca agtgttgcct gctaacgcgg ttgatgaaca
      961 tcaaacaaaa gtgattagta ttgttcgtca aactattaaa ctcgaagaac aagccgcgca
     1021 aaaacgtgtc attaccatta atcgtcaagg taaagattat aaaattggtg tgattaaatt
     1081 gccaactttt tatgccgatt ttgccgctat gcaagcaggt gatccaaatt atcgtagcac
     1141 cactcgtgat gtggctaaat taattgaaga attaaaagcc gagcaaattg acggcatggt
     1201 cttagattta cgcaataatg gtgggggttc tttaagtgag gctaattcct taattggttt
     1261 atttatagaa actggcccaa ccgttcaagt taaaaatgcc aacttggaag tggagttatt
     1321 aggcgactct aatcctgcgg ttgcttatga tggcccactg gtggtaatga ccaaccgttt
     1381 atcggcttcg gctgccgaaa ttgttgcagg tgctatacaa gattatggtc gtggtttagt
     1441 ggttggttct accacttatg gtaaaggtac agtgcaagcg ttacgcgaat taaatcatgg
     1501 tcaattaaaa ctgaccgaag ctaagtttta ccgtatttcg ggcgcaagca cccaacacaa
     1561 aggggttgag gcagacattc acttcccaaa tatttttcaa gggggtgata ttggcgaatc
     1621 ttatttagaa aacgccttac cgtgggatac aatttcacct gctcgatatt taccttattc
     1681 gggttttagt cgtcaattgc ctattcttaa attaaagtcg caacaacgtc aaacaagcaa
     1741 tccagatttt aaatatttaa atgctcaaat tacgttggct gaaagtataa aaaaagaaac
     1801 aaccatttct ttaaacgaag ccaagcgtaa aaaagaacag gaagaactgg ataacaaacg
     1861 tcttgcaatt gaaaatgaac gtcgtgccgc taaaggccaa gagttattaa aaacatggcg
     1921 cgaagctgag gcagcaaatg gcgacgataa agaagacgag ttagctgata ataacaaaaa
     1981 agaaaaaccc gaagacgagg catttgtcac tgaggcggct gaggtgttat tggatgccac
     2041 tttaaccgaa aaccccaaag aaacaattgc taaaggccgt taaactacgc gccgtaaatg
     2101 gtcagttggc tgtaaatacc aatataccca tgccgttaac cactgtcctg ttgtgagcag
     2161 gacaggtgtt aaccgccttt cgtattctgg attttgtgta tttacttctt caaattcatc
     2221 taaaattggc cacaaatctg ccgcacaggc atacacatca ccttcaatcc aactatcccc
     2281 atctacaaca gcaggataat aactgacttg atacaaatgc cctctcactt tagcatgacc
     2341 aataaattca gcgtgttgtg ctaaccataa acccattgga tgggtggatt gttgacgtaa
     2401 cgtgccataa acaaataaat aggtgttttc catgtcttta cctttagtat tagcgtctac
     2461 atcaattttt agacgcgagt tattaacacg tttaaccagc gattttagtc aagatagccc
     2521 aaatgttgat gaatcgccac ttgctaatga atctgcgcca caattagccc aacgtttggc
     2581 catactcaaa gcgcaagcat taacaacaca atatccacgt catttaatta ttggttctga
     2641 ccaagtggct agttttgaca ataaacttat tggtaaacca catacccacc aaaatgctat
     2701 tcaacaatta ttaactttta gtggtcaacg tgtcgatttt tatac
//
LOCUS       DAOQMB010000309         2736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_21853_length_2736_cov_15.525550, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000309 DAOQMB010000000
VERSION     DAOQMB010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21853_length_2736_cov_15.525550"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /gene="metG"
                     /locus_tag="PLY05_09235"
     CDS             complement(<1..1583)
                     /gene="metG"
                     /locus_tag="PLY05_09235"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020443732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNC03522.1"
                     /translation="MTRRLLVTSALPYANGSIHLGHLVEYIQTDIWVRFQKSRGNDCI
                     YVCADDAHGTAIMLKAEQNGISPEQQIANVKAEHEADFAGFHIAFDNYHSTHSEENRE
                     FSRFIYLKNRDAGHIATRPIRQLFDPEKQLFLADRFIKGECPKCGEKDQYGDSCEKCG
                     ATYAATELKNPYSTISGATPVEKESEHFFFKLPDFEAMLKEWTRSGRLQPEVANKLGE
                     WFDSGLADWDISRDAPYFGFEIPDAPNKYFYVWLDAPIGYMASFKNLCDKRGLNFDDY
                     WALDAESKTEVYHFIGKDIVYFHALFWPAMLDGAGFRKPTGIFAHGFLTVNGQKMSKS
                     RGTFIKARSYLDNLNPEYLRYYFASKLSASVEDIDLNLEDFMFKVNSDLVGKVVNIAS
                     RCAGFINKQFDGLLSASCAEPELLAEFVNAGTTIAEHFESRDFGKAVREIMALADKAN
                     QYIDEKKPWALAKDPANKEQVQAVCSVGLNLFRQLITYLSPILPITAAASQQFLNVDS
                     LAWHAREQLLLGHKINAFTP"
     gene            1694..>2736
                     /gene="apbC"
                     /locus_tag="PLY05_09240"
     CDS             1694..>2736
                     /gene="apbC"
                     /locus_tag="PLY05_09240"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HNC03523.1"
                     /translation="MSISRSKVEAVLKQHQDPYLNKDYGQLVESISIENEQVQVILTL
                     PYPFASLGRALEQQLADAIQSKTGAKQVKVQLQLDVPRAKPQEGVEGVPPIKNVIVVA
                     SGKGGVGKSTTAVNLALALAQEGATVGVLDADIYGPSQPTMLGLSGQTPKIQDNAFVP
                     LQAHGLQVMSIGFLTGDDNTPVVWRGPKATGALIQLFGQTAWHDLDYLIVDMPPGTGD
                     IQLTLAQRIPVVGSVIVTTPQDIALLDAKKGIEMFRKVNIRVLGVVENMSLHICSNCG
                     HAEAIFGAGGGERIANDYQTELLGQLPLDKVIREQTDAGKPTVLAEPNGRISEMYTDI
                     ARKIAAKLALEDSV"
BASE COUNT          841 a          550 c          548 g          797 t
ORIGIN      
        1 ggggtaaagg cattaatttt atgacccaac aataattgct cacgtgcgtg ccacgccaaa
       61 gaatcaacat tcaaaaattg ctgactggct gcggctgtga taggcaaaat aggggataaa
      121 taagtgatta attgacggaa taagtttaag cccacactac aaaccgcttg tacttgctct
      181 ttgttagcag ggtctttggc taatgcccac ggctttttct cgtcaatata ttggttagct
      241 ttgtcggcta aggccataat ctctcgaacg gctttgccaa agtctcgact ttcaaaatgc
      301 tcggcaatgg ttgtacctgc attaacaaat tcagccaata gttcaggctc agcacagctt
      361 gccgataata aaccatcaaa ttgtttatta ataaaacctg cacaacgact ggcaatgtta
      421 acgactttac ccaccaaatc actgtttacc ttaaacataa agtcttcaag gttaaggtca
      481 atgtcttcaa cactagccga taatttagag gcaaagtaat aacgcaaata ttcaggattc
      541 aagttgtcta aatagctacg tgctttaata aatgtaccgc gtgatttaga cattttttgg
      601 ccatttaccg ttaaaaagcc atgtgcaaaa ataccagttg gtttacggaa acccgcacca
      661 tctaacatgg caggccaaaa tagcgcgtga aaataaacaa tatctttacc aataaaatga
      721 taaacttcgg ttttgctttc ggcatctaat gcccaataat catcaaagtt taggccgcgt
      781 ttgtcacaca aattcttaaa actggccatg taaccaatag gtgcgtctaa ccacacataa
      841 aaatacttat taggtgcatc aggaatttca aagccaaaat agggcgcatc tctcgatata
      901 tcccaatcgg ctaaaccact gtcaaaccat tcacctaatt tattagccac ttcaggttgc
      961 aagcgaccag aacgcgtcca ttctttgagc atggcttcaa agtctggcaa tttaaagaaa
     1021 aaatgttctg attctttttc aacaggtgtt gcaccagaaa tggtggagta aggatttttt
     1081 aactcagttg ctgcgtaagt cgcaccgcat ttttcgcaag agtcaccgta ttggtctttc
     1141 tcaccacatt taggacattc acctttaata aagcggtctg ccaaaaacaa ttgcttctca
     1201 ggatcaaata attggcgaat agggcgagtg gcaatatggc cagcatcacg attttttaaa
     1261 taaataaaac gtgaaaactc acggttttct tcggaatgtg tggagtgata attatcaaat
     1321 gcaatatgaa agcctgcaaa atcagcttca tgctctgctt taacattggc aatttgttgt
     1381 tctggactaa tgccgttttg ttctgctttt aacatgattg ccgtgccgtg agcatcatcc
     1441 gcacacacat aaatacaatc attgccccga gatttttgaa aacgtaccca aatatcggtt
     1501 tggatatatt ctactaaatg acctaaatga atagagccat tggcataagg taaagcactg
     1561 gtcactaata aacggcgtgt catatttctc acactactca tatatgaatt taaaaataaa
     1621 gttgcctact ataaccgata ttgaaaatac ggtagcatta ccagccgaaa attttcttta
     1681 ttgagtgaat tttatgtcga tttcacgttc caaagtcgaa gctgtactca aacagcatca
     1741 agacccgtat cttaataaag attatgggca gttggttgag tcgatttcta tcgaaaatga
     1801 acaagttcaa gtcattttaa ccttgccgta tccttttgca agtttaggcc gtgccttaga
     1861 gcaacagttg gccgatgcta ttcaaagtaa aactggtgca aagcaagtta aagttcaatt
     1921 acagcttgat gttccccgtg ctaaaccaca agaaggcgta gaaggggttc cacctatcaa
     1981 aaacgtaatt gttgttgcat caggtaaagg cggtgttggc aaatccacaa ctgccgttaa
     2041 cttagcttta gccttagccc aagaaggtgc gactgttggt gtgcttgatg ccgacattta
     2101 tggcccaagc caaccaacga tgttgggttt aagcggccaa acacccaaaa ttcaagataa
     2161 tgcttttgtt cctttgcaag cacatggctt acaagtgatg tcgattggct ttttaacagg
     2221 tgatgataat acgcctgttg tatggcgagg cccaaaagcg acaggtgcgt taattcaatt
     2281 atttggtcaa acagcatggc atgatttgga ctatttaatt gtcgatatgc caccgggtac
     2341 aggtgatatt caattaacct tggcacaacg tattcctgtg gtgggttcag tgattgtcac
     2401 tacgccacaa gatattgctt tattagatgc taaaaaaggc attgaaatgt tccgtaaggt
     2461 caacattcgg gtgttgggtg tggttgaaaa tatgtctttg catatttgtt ctaactgtgg
     2521 tcatgcagaa gcaatttttg gcgcaggtgg tggtgagcgt atcgctaacg attatcaaac
     2581 tgaattatta ggccaattac cacttgataa agttattaga gagcaaactg atgctggtaa
     2641 accaaccgtt ttagctgagc caaatggtcg tattagtgaa atgtacaccg atattgcgcg
     2701 taaaattgcc gcaaaattag ctttagaaga cagtgt
//
LOCUS       DAOQMB010000310         2723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22030_length_2723_cov_16.104948, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000310 DAOQMB010000000
VERSION     DAOQMB010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22030_length_2723_cov_16.104948"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1957)
                     /gene="dxs"
                     /locus_tag="PLY05_09245"
     CDS             complement(74..1957)
                     /gene="dxs"
                     /locus_tag="PLY05_09245"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNC03524.1"
                     /translation="MMFYEIPLNRPNTPLLDSVPYPSDLRKLSLSSLPQLADELRAYL
                     LWCTGQTGGHFGAGLGVIELTIALHYVYDTPFDRLLWDVGHQAYPHKILTGRKEQMLS
                     MRQKDGLAAFPNRAESEYDTFGVGHSSTSISAALGMSLAARQQNSGRKVAAIIGDGAM
                     TAGMAFEALNDAAHNKADMLVILNDNQMSISHNVGGLSNYLARIWSSKQYIALREEGK
                     KVLATMPTAWNFARRTEESVKHMTASTGTLFEAIGFNTVGPIDGHDVIELVRVLRNLR
                     KAKGPQLLHLYTTKGKGFAPAEADPIGYHAITKIAPASERSTSPSPKKPKYSDVFGEW
                     LCDMAAEDSRLIGITPAMCEGSGMVKFAKQFPNRFHDVAIAEQHAVTLAAGMACEGLK
                     PVVAIYSTFLQRGYDQLVHDVALQNLDVTFGIDRAGLVGEDGPTHAGAYDYAYMRTIP
                     NIVICAPADENECRQLLYTAYQHQGCAAVRYPRGTGTGVHIDTQMTAIPIGKGEIKRL
                     GQKVAILAFGSMLSPSLQAAENLNATVANMRFVKPLDEQLIRELAQEHSLLVTVEEHA
                     IMGGAGSAVNEFICAQGLGISVLNLGIPDAFIEHASHSDMLKACGLDAQGIINTIKQR
                     IAV"
     gene            complement(2047..>2723)
                     /locus_tag="PLY05_09250"
     CDS             complement(2047..>2723)
                     /locus_tag="PLY05_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNC03525.1"
                     /translation="SAAGSVELVHCYSLVHDDLPCMDDDDLRRGVPTCHKAFDEATAL
                     LAGDTLQALAFVALSQSTLSDQQKSQQVQKLALASSDMAVGQALDLAGEGQQLSVAQL
                     EQIHRHKTGALIKASVLMGAIAAGCTDEATLQALEIYANKLGLAFQVHDDVLDVIGDT
                     QKLGKQAGADAELNKATYPALLGLSQAQQLATELHDQAIAAIKPLGCKANALVWLADF
                     LVDRDH"
BASE COUNT          771 a          594 c          601 g          757 t
ORIGIN      
        1 aacctaaata agtaggttta gatagatttt tcatatacag tgtgtaaccc taattaacaa
       61 aagtgagtga tttttatact gctatacgtt gtttgatggt gttgatgatg ccttgagcat
      121 ctaatccaca ggcttttaac atgtcgctgt ggcttgcatg ttcgataaat gcatcaggaa
      181 tgcccaagtt gagcacagag atgcccaagc cttgtgcgca aataaattca tttaccgcgc
      241 tgccagcgcc acccataatt gcatgttctt caaccgtcac caataaacta tgttcttgcg
      301 caagttcacg aatcagttgt tcgtccaatg gcttcacaaa acgcatattg gcgacagtgg
      361 catttagatt ttcggcagct tgtaggctcg ggcttaacat actaccaaac gctaaaatgg
      421 ctactttttg gccaagtcgt ttaatttcgc ctttaccaat agggatagcg gtcatttgtg
      481 tgtcgatatg aacgcctgtc cccgttccgc gtggataacg taccgccgca cagccttgat
      541 gttggtaagc ggtgtacaat aattggcgac attcattttc atcagcagga gcacaaatga
      601 caatattggg aatggtgcgc atataggcat aatcataagc gcctgcatga gttggcccgt
      661 cttcacccac taaaccagca cgatcaatac caaaggtaac gtctaaattt tgcaaggcaa
      721 catcgtgaac tagttgatca tagccacgtt gtaaaaaggt tgagtaaatg gcaaccacag
      781 gctttaaacc ttcacaagcc ataccagcag caagggtaac ggcatgttgt tcagcaatgg
      841 ctacgtcgtg aaagcgatta ggaaactgtt tggcaaattt gaccatgcca gagccttcgc
      901 acatggcagg agtaatgcca attaaacgag agtcctcagc ggccatatca cataaccact
      961 caccaaacac atctgaatat ttaggctttt tcgggctagg gctggtgctg cgttcactgg
     1021 ctggtgcaat cttggtaatg gcatgataac caattgggtc agcttcggca ggggcaaagc
     1081 ctttgccttt ggtggtatat aaatgtaata actgagggcc tttggctttg cgtaaattgc
     1141 gtaatacccg aactaattca atgacatcat gaccatcaat tgggccaact gtgttaaaac
     1201 caattgcctc aaataatgtg cctgttgagg cggtcatgtg tttgacactt tcttcggtac
     1261 gacgagcaaa attccatgcg gttggcatcg ttgctaagac ttttttgcct tcttcacgca
     1321 aggcaatata ttgtttgctt gaccaaatgc gtgctaaata attcgataaa ccaccgacat
     1381 tatgagaaat tgacatctga ttgtcattca aaatgactaa catatctgct ttgttatggg
     1441 cggcatcgtt gagcgcttca aaggccatgc ctgcggtcat tgcaccatcg ccaataatgg
     1501 cggcaacctt tcgacctgag ttttgttggc gtgcagccaa agacatgcct aacgcggcag
     1561 aaatggaggt tgaagagtga ccaacgccaa aggtgtcata ttctgattct gcgcgattag
     1621 gaaaggcggc taaaccatct ttttggcgca tagatagcat ttgttctttg cggccagtta
     1681 aaattttgtg tggataagct tgatgaccca catcccataa caaacgatca aacggtgtgt
     1741 cataaacata atgtaaggca atggtgagtt cgatcacccc taaacctgca ccaaaatgac
     1801 cacccgtttg acccgtacac cacaataaat aggcacgtaa ttcatccgct agctgtggca
     1861 aactggataa ggagagtttg cgtaaatcgc tgggataagg cacggagtcc aataaagggg
     1921 tattgggacg atttaatgga atctcataaa acatcatagc tttattaatc aaatttggga
     1981 cttgggctaa ttatagcgtt ttaggctata ttaggtattg tttattgctg tactaaatga
     2041 caagtcttaa tgatctctat caaccaaaaa atcagctagc cacactaagg cattggcctt
     2101 acaacctaaa ggtttaattg cggcaatggc ttgatcatga agctcagtgg ccaattgctg
     2161 tgcttggctt aatcctaata aggcaggata agtggcttta ttaagttcgg catctgcgcc
     2221 tgcttgtttg ccgagttttt gtgtatcacc aatcacatcc aacacatcat catgtacttg
     2281 aaaggccaaa ccaagtttat tggcataaat ttcaagagct tgtagtgtgg cttcatccgt
     2341 gcaaccagca gcaatcgctc ccattaacac acttgccttg attaatgcac ctgttttatg
     2401 gcgatgaatt tgctcaagtt gagccacaga aagctgttga ccttcacctg ccaaatcaag
     2461 ggcttgacca acggccatat cactactggc taaagcgagt ttttggactt gttggctttt
     2521 ttgttggtcg gataatgtgc tttggcttaa agcaacaaaa gcaagagctt gtaaggtatc
     2581 tcctgctaat aaagctgtgg cttcatcaaa tgctttgtga caagtgggca cgccacggcg
     2641 taaatcatca tcatccatgc aaggcagatc gtcatggact aaagaataac aatgcactaa
     2701 ttcaacagac cctgccgcag agt
//
LOCUS       DAOQMB010000311         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22228_length_2708_cov_48.937429, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000311 DAOQMB010000000
VERSION     DAOQMB010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22228_length_2708_cov_48.937429"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2708)
                     /locus_tag="PLY05_09255"
     CDS             complement(<1..>2708)
                     /locus_tag="PLY05_09255"
                     /inference="COORDINATES: protein motif:HMM:NF032893.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLxRFG domain-containing protein"
                     /protein_id="HNC03526.1"
                     /translation="RFSRAEDKAKAAKDYVLDSVRIKRQVAENLHAKAELGWWSKTIG
                     TQLNTAKKFKDFDRVYKLLQRLLQHQSSDVMDALEVAPTLLEKLDDWQDLKNQVKAIG
                     RNAWQGLKHKLTDAVTDRMAVAKVIFSETLAKKPLSQAELEQKLTPQQLKIYEEARAA
                     INVSLKNTAKSEIIRSLLQNDAINFDTVEKLLNQQTGFDEFIDEMLENVGARISNAQE
                     ELSQAETDVEKQAIKDELKRLADMYNSISDVNTKVYKLIDEAYAPLMRFGDYALTMRD
                     KTTGKVLSFMMFESKAERNRMVNYLGTEYGDTVTLETDTMSTEDFKMFKGVTPETVAL
                     FAKELGLDKNEAYQGYLKLAIPATSALTRRIQRKGTAGYNEDIQRVVSAFVMSNARYS
                     AKNIYLAKADREISEIESGASVRDQAKKMRDNTIEPVDTFAWLRDLLFVWNLGGSVMF
                     AFLNMTQPFLQTIPMLSQYVNTSFATKHVWRGIGVAGKAMATGNAPKGYEAEYKRAVH
                     EGVVDPQNTFMLTGLERGGTGTTSKVWTGTKHILGIFAQMSESLNRKAVFIASLEVAN
                     AKGDAWLKQKGFDSAYDFAKDVIAQTQGVYNKANSSNWAHNPVGAPLLVFKQFSINYV
                     EQMIRMWKNQSAGEEGKKAVLLAMMMLFSLAGGMGLPFIKDIFDGSETIAAFMGNPVN
                     IEREMRLMGKQYLGETTTGALMDGWLNKLVIGEVLGADVQSRTGMGDLIPATNFFNKT
                     IYGTDQQVKEGMSIFGAAGGLGDKVGDSLNFMANDEWGDAFINILPRFATSMYRGVEM
                     MTTGETTDSKGRKLFDTTFGEGLVKFLDSQPVRMAELSRARGLEYADIAIQRSKIDYY
                     RKKWIKAAMDEDFAKIEEIEQDIADWNEENEKYPVIFDRQRAYQSA"
BASE COUNT          717 a          656 c          469 g          866 t
ORIGIN      
        1 ttggccgatt gataagcacg ctgacggtca aaaataacag gatatttttc gttttcttca
       61 ttccaatctg caatatcctg ttctatttcc tcaatcttgg caaaatcttc atccattgcc
      121 gccttaatcc attttttacg gtaatagtca atcttgctac gctgaatagc aatatctgca
      181 tactctaatc ctcttgctct tgataactca gccatgcgta caggttgaga atccaagaac
      241 ttaactaacc cctcaccaaa agtcgtatca aataattttc ttcctttact gtccgtggtt
      301 tcgcctgtgg tcatcatctc aacaccgcga tacatactgg ttgcaaagcg aggtaagata
      361 ttaataaacg catcgcccca ttcatcattg gccataaaat taagtgaatc acccaccttg
      421 tcgcctaatc cgccagcagc cccaaaaatg gacatcccct ctttgacttg ttggtcagtg
      481 ccgtaaatgg ttttattaaa aaagtttgtt gcaggtatta aatcccccat gcctgtccta
      541 ctctgaacgt cagcccccag cacctcacca atcactaatt tattgagcca tccatccatt
      601 agtgcgcctg tcgtggtttc accaaggtac tgcttgccca ttaagcgcat ttctctctcg
      661 atgttgactg gattccccat aaatgccgcg attgtttcag aaccatcaaa aatatcttta
      721 ataaatggca atcccatacc gccagccaaa gagaaaagca tcatcattgc aagtaatact
      781 gcttttttac cttcttctcc tgcactttgg ttcttccaca tacgaatcat ttgctcaaca
      841 tagttaatgc tgaactgctt aaacacaagc aatggcgcac ccacagggtt atgcgcccaa
      901 ttactactgt tagctttatt gtaaacgcct tgtgtttgag caattacatc ttttgcaaaa
      961 tcataagcag aatcaaaccc tttttgtttt aaccatgcgt cacctttagc atttgccact
     1021 tctagcgagg caataaatac ggctttacgg tttaaagatt cgctcatctg agcaaatata
     1081 ccaagtatat gttttgtacc tgtccatact ttacttgttg tacctgtgcc gccacgctct
     1141 aaacctgtca acataaaggt attttgtggg tcaacaacac cctcatgtac agcgcgtttg
     1201 tactcagctt cataaccctt tggcgcgttg cctgtggcca ttgccttgcc tgcaactcca
     1261 atccctctcc agacgtgttt tgtcgcaaag ctagtattaa cgtattggct caacataggg
     1321 attgtttgta aaaatggttg ggtcatattt aaaaaggcaa acatcaccga gccgcccaag
     1381 ttccacacga acaacaagtc acgcaaccat gcaaaagtat caacaggctc aatcgtgttg
     1441 tcgcgcattt tcttggcttg gtctcttacc gatgcaccgc tttcaatctc actgatttcc
     1501 ctatctgcct tagcaagata aatgtttttg gcagaataac gcgcattact cattacaaat
     1561 gcggaaacga cacgctgtat atcttcgttg tagcctgctg tgcctttacg ttggatgcgt
     1621 cttgttaatg cgctagtggc aggaatagca agctttaaat aaccctgata cgcttcgttt
     1681 ttatcaagac caagctcttt ggcaaataat gcgactgtct caggcgttac ccccttaaac
     1741 attttaaaat cttcggtact catggtgtcg gtttctaaag tgacggtatc gccatactct
     1801 gtacctaagt aattcaccat gcgattgcgc tcagccttag attcaaacat cataaatgat
     1861 aaaaccttac ctgtggtttt atcacgcata gtcagggcgt aatcaccaaa acgcatcaaa
     1921 ggtgcgtatg cttcatcaat cagcttgtaa accttagtat ttacatcgct aattgagtta
     1981 tacatatcgg ccaatcgttt taattcatcc ttaatggctt gcttttcgac atcagtttca
     2041 gcctgtgata attcctcttg tgcattgcta atacgcgcac ctacgttttc aagcatttca
     2101 tcaataaatt catcaaagcc tgtttgttgg tttaataact tttcaacggt atcgaagtta
     2161 atcgcatcgt tttgtaatag gctgcgaata atctcagact ttgccgtgtt ctttaacgat
     2221 acgttaatcg ctgctcgcgc ttcttcataa atcttcaatt gttgtggtgt tagcttttgc
     2281 tctaactcag cttgtgataa aggttttttg gctaatgttt cactaaaaat aaccttagca
     2341 actgccattc tgtcggtaac tgcatcggtc agtttatgct ttaaaccttg ccaagcgttt
     2401 ctaccaatag ctttaacttg attcttaagg tcttgccagt cgtctaactt ttcaagcaaa
     2461 gttggggcaa cttctaaagc atccataaca tcagaagatt ggtgttgtaa taaacgctgc
     2521 aacagtttgt acactcggtc aaagtcttta aactttttag cagtgtttaa ttgtgtgcca
     2581 atggttttac tccaccagcc aagctctgcc ttagcgtgta agttttcagc cacctgtctt
     2641 ttaatccgta ccgaatctaa aacatagtct tttgctgctt tggctttatc ttcggcacga
     2701 ctaaagcg
//
LOCUS       DAOQMB010000312         2707 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22243_length_2707_cov_19.158748, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000312 DAOQMB010000000
VERSION     DAOQMB010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22243_length_2707_cov_19.158748"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /gene="leuA"
                     /locus_tag="PLY05_09260"
     CDS             complement(<1..1721)
                     /gene="leuA"
                     /locus_tag="PLY05_09260"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC03527.1"
                     /translation="MMLKNPATKYRAFPAINLPNRQWPSQTITHAPIWMSTDLRDGNQ
                     SLFEPMNGERKMQMFKMLCAIGFKEIEVAFPSASQTDFDFVRTLIEDGHIPDDVTIIV
                     LTQAREHLIRRTVESLRGAKKAIVHVYNATSQPFRDVVFNMSKDEVVTMAVNAVALIK
                     ELTAAQPDTQWQLEYSPETFTATELDFAKQVCDAVTETWGATPDNKVILNLPATVEVA
                     TPNVYADQIEWMHRYLARRDSVIISVHPHNDRGTAVAATELALMAGAERVEGCLFGHG
                     ERTGNVDLVTLALNLYSQGISPNLDFSDVNSVARTVEYCTQLPVHPRHPYVGDLVFTA
                     FSGSHQDAIKKGFSAQQADSYWNMPYLPIDPADVGRSYDSVIRVNSQSGKGGVSYLLE
                     NSYGIVMPRRLQVEFSGVVQQHTDQHGCEVNANDIWQLFDQTYLNQQASIAYQEHHLF
                     EHDKQQGIRLVLNINGTTQILTGHGNGPIDASVNALQHAGIHIQVRSYEERSINIPSP
                     QPSPTGRGSNEGSHAQASAFIEVSQHGTTASQYGVGIDSNIITASVKALISGANRVMG
                     QNLASKVG"
     gene            1980..2447
                     /locus_tag="PLY05_09265"
     CDS             1980..2447
                     /locus_tag="PLY05_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009394930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNC03528.1"
                     /translation="MQLDRYDRQILELLQQDSRISNQDLADKIGLTPSPCLRRVKTLE
                     DLGFITAYRALVDAKKLGLSLMALIHISMDQHTPERFKYFEESIVDIPEVLECLLITG
                     QSADYQLKVVVKDMDGFQELLLNRITRITGVTGVHSSFVLRKVMDRTALPVSL"
BASE COUNT          781 a          569 c          572 g          785 t
ORIGIN      
        1 cccactttac tagccaaatt ttgccccatc acacggtttg caccgctaat taacgctttt
       61 accgatgcag taataatatt gctatcaata ccaacaccat attgactggc cgttgtgcca
      121 tgttgactca cctcaataaa ggcactggct tgagcatgag aaccttcatt actccctctt
      181 ccagtgggag agggttgggg tgaggggata ttaatcgaac gctcctcata actacgcact
      241 tgaatatgaa tgcctgcatg ttgcaaggca ttgacactcg catcaattgg gccattacca
      301 tgacctgtca aaatttgggt tgtgccatta atatttaaca ccaaacgaat gccttgttgc
      361 ttgtcatgct caaacaaatg atgttcttga taagcaatac tcgcttgttg atttaaataa
      421 gtttggtcaa acaattgcca aatatcgttg gcattgactt cacaaccatg ttgatcggta
      481 tgctgttgta ccacacccga aaactccact tgcaaacgac gtggcatgac aatgccatag
      541 ctgttttcta acaagtagga cacgccacct ttgcccgatt ggctattgac ccgaatcacc
      601 gaatcataac tacggccaac atcggcaggg tcaatcggta agtagggcat attccaatag
      661 ctatcggctt gctgcgcgga aaaacctttt ttaatcgcat cttggtgtga acccgaaaac
      721 gcggtaaaga ccaaatcacc gacatacgga tgacgtggat gcacaggcaa ttgcgtgcag
      781 tattcaacag tacgtgctac cgaattaaca tccgaaaaat ctaaattggg actaatgcct
      841 tggctatata aatttaatgc caaggtcact aagtcaacat tacctgtacg ttcaccatga
      901 ccaaacaaac agccttccac acgttctgca cctgccatca atgccaattc ggtggcagca
      961 acggctgtgc cacggtcatt atgcggatga acactgataa tcacgctatc acgacgggct
     1021 aaataacggt gcatccactc aatttggtcg gcataaacat taggtgttgc cacttctacc
     1081 gtcgcaggca agtttaaaat cactttgttg tctggcgttg ccccccatgt ttcggtgacg
     1141 gcatcacaca cttgtttagc aaaatctaac tcggtggcgg taaaggtttc tgggctgtat
     1201 tccaattgcc attgtgtgtc tggttgtgca gcggttaact ctttgattaa agccacggca
     1261 ttgacagcca tcgtcaccac ttcatcttta ctcatgttaa agaccacatc gcggaacggt
     1321 tgagaggtgg cattgtaaac atggacaatg gcttttttag ccccacgcaa cgactcaacg
     1381 gtacgacgaa tcaaatgttc acgcgcttga gttaagacaa taatcgtcac gtcatcagga
     1441 atatgaccat cttcaatcaa ggtgcgcaca aaatcaaaat cggtttgtga ggcagaaggg
     1501 aatgccactt caatttcttt aaaaccaatc gcacacaaca tcttaaacat ttgcattttg
     1561 cgttcgccat tcattggctc aaacaatgat tgattaccat cacgcaaatc agtgctcatc
     1621 caaatggggg catgagtgat ggtttggctt ggccattggc gattgggcaa attaatggca
     1681 ggaaaagcac ggtatttagt cgcaggattt tttaacatga tggcatctcg tttaagcctc
     1741 acacggtttg tttagttctc tctccctttg ggagagagct agagtgaggg taattaattt
     1801 ttcattgctt gtatgataaa taaaactcaa gagcagagga ttgcgaaata aggcttaatt
     1861 tgcagctttt aatataaaaa attgctaaaa ataggttttt attgaaatat tattgcgtaa
     1921 atatattttt tgttggtagg atgggttgag gaacgaaacc catcattaaa ggttggctta
     1981 tgcaactcga ccgctacgat cgccaaattt tagagctatt gcaacaagat agtcgcatca
     2041 gcaatcaaga ccttgccgat aaaattggct taacgccttc accgtgtttg cgacgcgtca
     2101 aaactttaga agacttaggt tttattaccg cctatcgtgc tttagttgat gctaaaaaat
     2161 tgggattaag cttaatggcg ttgattcata tttcgatgga tcagcatacg cccgaacgtt
     2221 ttaaatattt tgaagaaagc attgttgata ttcccgaagt attagagtgt ttgttaatta
     2281 ctggtcaatc tgctgattat caactcaaag tagtggtcaa agatatggat ggttttcaag
     2341 agctattgct caatcgtatt acccgaatta caggtgtaac gggtgtgcat agtagttttg
     2401 tgttgcgtaa agtcatggat cgaactgcgt tgcctgtgag tttataatgc ggacttacgc
     2461 atttcaccga aattagcgcg ttttgcgagc taaaaatcgt tgaaaacgat tgtttggcga
     2521 gtaaataagc gataaccgag taagtcctaa aatgtcctca ttaatttatc tctcttacca
     2581 acaacatgaa gtgatattag accgagactt tcaatttatt gttttggcta atggtttgaa
     2641 ttggctgtcg gagtcaacat atcgtttaat gatgggaaaa ttggccgata tttgccaaac
     2701 agaaaga
//
LOCUS       DAOQMB010000313         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22351_length_2701_cov_14.620937, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000313 DAOQMB010000000
VERSION     DAOQMB010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22351_length_2701_cov_14.620937"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLY05_09270"
     CDS             complement(<1..187)
                     /locus_tag="PLY05_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03529.1"
                     /translation="MIQITTTGIGQTLKSIVFLCSLLSLPSLSYAQDDDIEELRQPTP
                     VVSEKPKAETPKTVEKKT"
     gene            complement(184..483)
                     /locus_tag="PLY05_09275"
     CDS             complement(184..483)
                     /locus_tag="PLY05_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012315618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNC03530.1"
                     /translation="MWYAIIAEDKPDSLSQRLAARPDHVARLKQLQAEGRLFVAGPHP
                     AIDSEDPAEAGFTGSLIIAEFASLTEAQAWAANDPYIAAGVYQQVTVKPFKKVLP"
     gene            complement(485..1027)
                     /gene="ispZ"
                     /locus_tag="PLY05_09280"
     CDS             complement(485..1027)
                     /gene="ispZ"
                     /locus_tag="PLY05_09280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein IspZ"
                     /protein_id="HNC03531.1"
                     /translation="MKQWLDFIPLFAFFATFKFLGIFPATAVLIGSSILVYGGLWLKE
                     RHLENGQKITLVATILFGSATLLLHDEAYLKWKAPIINWIFALAFLISMYIGKEPLVQ
                     KMLGKAFDMPATVWRKLNWAWIGFFIFAGASNAYVAFYFEKYWVDFKVFGSLAMTIIF
                     MIAQFALLSRYLRPELTQEK"
     gene            1085..1933
                     /locus_tag="PLY05_09285"
     CDS             1085..1933
                     /locus_tag="PLY05_09285"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNC03532.1"
                     /translation="MPSIDLHCHSFFSDGTQSPSQLVQQALAVQLQYLALTDHDSVNG
                     LAEAHQAAQGTNLTIINGVEISAVWQKQLIHIVGLGIDPQNAHLLAGLAEQAQARAKR
                     GKLIAEKLTQLGIANTWPEVLALAEGDENRVGRAHFAQCLLNRGVVNNLQKAFDKYLA
                     TGKPAAVPMPWTDMLMAIAWIQQAGGVAVLAHPARYNLSQTKLRALLADFKQAGGQAM
                     EVNTANEKVNIINNLAALSQRFELYASQGSDYHGTTMPWLKLGHFAQQPSICQPVWQL
                     LNINLN"
     gene            1961..2584
                     /locus_tag="PLY05_09290"
     CDS             1961..2584
                     /locus_tag="PLY05_09290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNC03533.1"
                     /translation="MSQYFHIHPDNPQPRLIKQAVQIIQNGGVIAYPTDSAYALGCHL
                     GDKNALERIIRIRQLTDKHHFTLLCRDLSDIATYAKVDNPTYRLLKAHTPAAYTFILT
                     ASSEVPRRLMHPKRKTIGLRVPDHAICQALLAELNEPLLTATLQLPNESEPLYDPEDI
                     FERLGKQLDLVINGGFGTLIPTTVVDLSESGNPIIIREGAGDISPFI"
BASE COUNT          863 a          518 c          525 g          795 t
ORIGIN      
        1 tggttttttt ctctaccgtc tttggtgttt cagctttggg tttttcactc acgacagggg
       61 taggttgtct taactcttca atatcatcat cttgtgcata acttaaggac ggcaatgaga
      121 gtaaactgca caaaaagaca atagatttca atgtttgacc tatacctgtc gtggtgatct
      181 gaatcatggt aatacttttt taaagggttt tacggtaact tgttgataaa cgcctgcggc
      241 aatataaggg tcattagcag cccatgcttg tgcctctgtt aagctagcaa attcagcaat
      301 aattaaactg cccgtaaaac ctgcttcggc ggggtcttca ctatcaattg caggatgtgg
      361 gccggcaaca aataaacggc cttcagcttg taattgtttt agacgagcaa catggtcagg
      421 acgagcagct aaacgttggc taaggctatc aggtttatcc tcagcaataa tggcatacca
      481 catattattt ctcttgagtt aattcgggac gtaagtagcg agataataaa gcaaattgtg
      541 caatcataaa aatgatggtc atcgctaaac tgccaaaaac tttaaagtcc acccaatatt
      601 tttcaaaata aaaggcaaca taggcattac ttgcccctgc aaaaataaaa aagccaatcc
      661 atgcccaatt gagtttacgc caaacagttg caggcatatc aaaggcttta cctaacattt
      721 tttgtactaa aggctctttg ccaatataca tgctgattaa aaaggccaag gcaaaaatcc
      781 aattaataat aggagctttc cattttaaat aggcttcatc atgtaataac agcgttgcac
      841 taccaaataa aatcgtggcg acgagggtta ttttttggcc attttcgagg tggcgctctt
      901 ttaaccacaa accgccataa accaaaatag agctgccaat taatacagcc gttgcaggaa
      961 aaatacctaa aaacttaaaa gtggcaaaaa aggcaaataa aggaataaag tcaagccatt
     1021 gtttcataat ctgatgcttt gtgggacaat ttagcgaatc ttaatcatct ttttgtcatc
     1081 tgtcatgcct tctattgatt tacattgtca tagttttttt tctgacggca cacaaagccc
     1141 cagtcaactt gtacaacaag cgcttgcagt ccaactacaa tacttagcat taaccgacca
     1201 tgatagcgtc aatggtttgg cggaagcgca tcaggcagcg caaggcacta acttaaccat
     1261 tattaatggc gtagaaattt cggcagtgtg gcaaaagcaa ttaattcata ttgttggcct
     1321 aggcattgac cctcaaaatg cacatttatt agcaggattg gcagagcaag cccaagcaag
     1381 agccaaacgc ggcaaattaa ttgctgaaaa attaacgcaa cttggtatag ccaatacatg
     1441 gccagaggtg ttggccttag ccgaaggcga tgaaaataga gtggggcgcg cacattttgc
     1501 ccaatgttta ttaaatcgag gcgttgtcaa caatttgcaa aaagcctttg ataaatattt
     1561 agccacaggt aaaccagcgg ctgtgcctat gccttggaca gatatgttaa tggcgattgc
     1621 atggattcaa caagcaggtg gggtagcagt gttggcacat cctgcacgtt ataacttatc
     1681 acaaaccaaa ttgcgtgcct tattagccga ttttaaacaa gcaggtggtc aagccatgga
     1741 agtgaatacg gccaatgaaa aagtgaatat tattaataat ttggcagctt tgtcacaacg
     1801 atttgaatta tatgcttcgc aaggcagtga ttatcatggt acaaccatgc cttggttaaa
     1861 gcttggccat tttgctcagc agccaagcat ttgtcagcca gtttggcagt tattaaatat
     1921 taatctaaat taatataata atatttaagg gtaaatatat atgagtcagt attttcatat
     1981 tcatccagat aatcctcagc cgcgtttaat taaacaagca gtacagatta ttcaaaatgg
     2041 cggggttatc gcttatccta ctgattctgc ttatgcttta ggctgtcatt taggcgataa
     2101 aaatgcttta gagcggatta ttcgtattag gcaattaacc gataaacatc attttacctt
     2161 attatgtcgc gacttatctg atattgccac gtatgccaaa gttgataatc ccacctatcg
     2221 tttattaaag gcgcatacgc ctgctgcata taccttcatt ctaacggcaa gtagtgaagt
     2281 gcctagacgc cttatgcacc caaaacgtaa gactattggt ttacgtgtac cagaccatgc
     2341 catttgccaa gccttattag cagagttgaa tgagccatta ttaactgcta cattacagtt
     2401 acctaatgaa tctgaaccct tatatgatcc cgaggatatt tttgaacgtt tagggaaaca
     2461 attagattta gtgattaatg gtggatttgg tacgttaata ccaaccacag tggtggattt
     2521 aagtgagtca ggtaatccaa ttataattcg tgagggtgca ggcgatatta gcccatttat
     2581 ttaatgagaa tatttcttaa ttacctaagt ttatcatcca taaaatgaga tatttatcat
     2641 aattaattta tttatataaa aaaaccgaaa aaaccattgc aaaaatgaaa tatacgagta
     2701 a
//
LOCUS       DAOQMB010000314         2701 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22352_length_2701_cov_13.831822, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000314 DAOQMB010000000
VERSION     DAOQMB010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22352_length_2701_cov_13.831822"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            406..777
                     /gene="clpS"
                     /locus_tag="PLY05_09295"
     CDS             406..777
                     /gene="clpS"
                     /locus_tag="PLY05_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HNC03534.1"
                     /translation="MSNMFVDELISIQMANHDDMANDGDTAVIEQTAKPQLKKPSFYQ
                     VILLNDDYTPMDFVVEVLEDFFAMNIEQATQVMLTIHHQGQAVAGLYPKDIAETKAQQ
                     VIDFARHNQHPLMCRVEQKPD"
     gene            906..>2701
                     /locus_tag="PLY05_09300"
     CDS             906..>2701
                     /locus_tag="PLY05_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002125880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03535.1"
                     /translation="MLSRDLEVSLNLAFRDARAKRHEFMTVEHLLLALLDNESAVRVL
                     KSCGGDIATLRRELSQFIEETTPKIPTKDTDRETQPTLGFQRVLQRAVFHVQSSGKKE
                     VLGANVLVAIFSEQESQSVYFLKKQNISRLDIVNFISHGISKQSDENKADDDANEQIQ
                     NQTDEDGELVPKSALAAYTSNLNELALAGKIDPLIGRDAEIERAAQILCRRRKNNPLL
                     VGEPGVGKTAIAEGLAWLIVSNKAPEPLAKSVVYSLDLGALLAGTKYRGDFEKRFKAV
                     LSELNKQQDAVLFIDEIHTIIGAGAASGGVMDASNLLKPVLVNGSLRCMGSTTYQEYR
                     GIFEKDRALARRFQKIDIPEPTVGETYQILKGLKSRFEEHHGVRYSEKALEVAAELAS
                     RYINERFLPDKAIDVIDEAGALQRLQPVSKRKKTIGVGEIEEVIAKIARIPPKSVSAN
                     DKEALRNLERDLKMTVFGQNDAITALSSAIKLSRAGLKAPEKPVGCFLFAGPTGVGKT
                     EVTKQLAKILGIELVRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTEAISKHPHCV
                     LLLDEIEKAHPEVFNLLLQVMDHGTLTDNNGR"
BASE COUNT          842 a          465 c          580 g          814 t
ORIGIN      
        1 ttagatgcta aacgtgcgat tcgtcaaggc gcagaattag ccgcgcagat ttaacgtaac
       61 ttcctcaaca taatccttcg gcttcgctca gggcgcattt ttgtattggt tgagcgaagt
      121 cggggtgatt gccctttgac ttcgctcagg gaacagagtc tgactgacat tagggtaata
      181 cacgatttaa aaccgcttac aaaataaata aaaaaattgc ttatttttca tcattaagct
      241 ttaaaatagc tgccgtttag cttttcattt ataagctatc ttttatcgta ctttcactct
      301 actttgtcgc aaaatacgaa attgcacttg agattttgca caattccacc cataactttg
      361 ttaaggctta tagaaatatg agcttatgtt tgtaggctgc ttgttatgag caatatgttt
      421 gtagatgaac taatatcaat tcaaatggcc aatcacgatg acatggcaaa tgatggtgat
      481 accgctgtta tagagcaaac agcaaaacca cagctcaaaa aaccatcgtt ttatcaagtg
      541 attttgctaa atgatgacta cacacccatg gattttgtgg tagaagtatt agaagatttt
      601 tttgcaatga atattgagca agcaacacaa gttatgctaa ctattcatca tcaaggacaa
      661 gcggttgcag ggttatatcc aaaagatata gctgaaacca aagctcaaca agtgattgat
      721 ttcgcgcggc acaatcagca ccccttaatg tgtcgagttg agcaaaagcc tgattaaaaa
      781 caggatttat aggtaaaggc ttaaattttg ctcaattaaa gcggctgtta caaaaggccg
      841 cttgatccaa gcaataaaaa taagcgataa ccatgtaagt cctacctaaa aatataggag
      901 ttgctatgtt aagtcgcgat ttagaagttt cattaaattt ggcctttcgt gatgcccgtg
      961 cgaaacgcca tgaatttatg accgttgagc atctcctatt agctttatta gacaatgaat
     1021 ccgcagtaag agtgcttaaa tcctgtggtg gcgatattgc aaccttaaga cgtgaattaa
     1081 gtcagtttat tgaagaaact acccctaaaa ttccgactaa agatacagat cgtgaaactc
     1141 aaccgacact gggttttcaa cgggtgttgc aacgtgctgt atttcatgtg cagtcatcag
     1201 gtaaaaaaga agttttaggt gcaaatgttt tagtcgcgat tttttcagaa caagaatcgc
     1261 aatctgttta tttcttgaaa aagcaaaaca ttagtcgttt agatattgtt aactttattt
     1321 cgcatggcat tagtaagcaa agtgatgaaa ataaagcaga tgacgatgca aatgagcaaa
     1381 ttcaaaacca aacagatgaa gatggtgaat tagtaccaaa gagtgcattg gctgcttata
     1441 ccagtaactt gaatgaattg gccttagctg gaaaaattga tccgctgatt ggtcgtgatg
     1501 ccgaaattga acgtgctgcc caaattctgt gtcgtcgtcg taaaaacaat cctttgttag
     1561 tgggtgaacc gggtgtgggt aaaactgcga ttgcagaagg cttggcatgg ttgattgtaa
     1621 gcaataaagc accagagcca ttagcgaaat cggtggttta tagcctagat ttaggtgcgt
     1681 tattggcagg cacaaaatat cgtggtgatt ttgaaaaacg ttttaaagcg gtattaagtg
     1741 aactaaataa acaacaagat gctgttttgt ttattgatga aatccatacc attattggtg
     1801 caggtgctgc aagtggtggt gtgatggatg cttctaattt acttaagcct gtcttagtta
     1861 atggttcgtt gcgttgtatg ggttcgacaa cttatcaaga gtatcgtggt atttttgaaa
     1921 aagatcgtgc tttagcgcgt cgtttccaaa aaattgatat tcctgagcca actgttggcg
     1981 aaacttatca aattttgaaa ggtttaaaat cacgttttga agagcatcat ggcgtacgtt
     2041 atagcgaaaa agccctagaa gttgcggctg aattagcaag tcgttatatc aatgagaggt
     2101 ttttacccga taaagccatt gatgtgattg atgaagcagg tgcattacaa cgtttacaac
     2161 ctgttagcaa acgcaaaaaa accattggtg ttggtgaaat tgaagaggtg attgccaaaa
     2221 ttgcccgtat tccaccaaaa tctgtctcgg cgaatgataa agaagcttta cgtaatttag
     2281 agcgtgatct caaaatgact gtatttggtc aaaatgatgc tattactgct ttatcttcag
     2341 cgattaaatt atcacgtgca ggtttaaaag cacccgaaaa acccgttggc tgtttcttat
     2401 ttgcagggcc tacaggtgtt ggtaaaaccg aagttaccaa acagttagcg aaaattttag
     2461 gcatagaatt agtgcgcttt gatatgtccg aatatatgga gcgtcatact gtttcgcgtt
     2521 tgattggtgc gccaccaggt tatgttgggt ttgatcaagg cggcttatta accgaggcga
     2581 tttcaaaaca tcctcattgt gttttattgc tagatgaaat tgaaaaagcc catccagaag
     2641 tctttaactt gttattacaa gtgatggatc atggcacgtt aactgataac aatggccgta
     2701 a
//
LOCUS       DAOQMB010000315         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22403_length_2698_cov_10.315551, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000315 DAOQMB010000000
VERSION     DAOQMB010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22403_length_2698_cov_10.315551"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLY05_09305"
     CDS             <1..1077
                     /locus_tag="PLY05_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC03536.1"
                     /translation="MSDILVTGASGFIGKFLILALVKKGHRVFALFRKPTEQFPQLIA
                     WLATKGVAEAQIVPIQGDLSVANVGISEENWQKMANVSVIYHSAAMFGWNLSEQQARQ
                     VNVKGVLELLTLAQQKLSLKQVVQVSGYMLTMTEHLQQLGIEGLAENANWSKIYQQVG
                     VYEASKIEAHFAIKRLSKQLNIPLTVIHPATVIGDHQQGELASQQPFYQTLVDLKAGK
                     LFAVPSGQGYRLPLGSVAYLTQFMANVIDYPQTINQEYVLADNSTPNLKQVLTVCAKA
                     IAIKPPMLSIPIPVLKCVLTSEFVAKKAGMSLEMLHFFRQESLATEATQQLANMMALP
                     LPSLEHCLQQTAKFVARQSLAKAS"
     gene            complement(1049..2374)
                     /locus_tag="PLY05_09310"
     CDS             complement(1049..2374)
                     /locus_tag="PLY05_09310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC03537.1"
                     /translation="MSYWWARLKPKSAVWRTTLVISIVLLVSQYLSLAFFWWNLYLPE
                     MRQHGHYVAVNLSLLRNAEDDVLQNPQALEMQAWVEKATGIEVVRNPEEFPTLKSKPF
                     AEFFTQELEEQLRRELHEPVDVYFEFKPTPRLWMYIPSMKDVWVREPLLFFAQYNPFI
                     IIAWVIGVPIMAIIAIVILVRQLNRPLKRLELAARRVAKGQHATMLEIKRGPSEIRAV
                     NAAFNQMTLEIQQAAKDRTVMLAGISHDLRTPLTRLRLTAEMMSDKELAEGMILDIED
                     MDAILEQFIAFMRDGSDEALEPCDINGILSEVAAQFSQQTAIEFEPTPQLPLIAVRRT
                     AIKRLVGNLVNNALRYAHPPIELNATYNREYLMISVRDHGTGIEESKIPELLQPFVRG
                     ESARTTQGSGLGLAIVARIVKMHKGLLDIKNHPEGGLEITVSLPLPKIA"
     gene            complement(2385..>2698)
                     /locus_tag="PLY05_09315"
     CDS             complement(2385..>2698)
                     /locus_tag="PLY05_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HNC03538.1"
                     /translation="ETVSFGEWELDLSTRQFRKNGEDVPLTTGEFAVLKALVQHPREP
                     LTRDKLMNLARGREWGAMERSIDVQVSRLRRLIEEDSTKARYIQTVWGVGYVFVPDGK
                     E"
BASE COUNT          786 a          547 c          579 g          786 t
ORIGIN      
        1 atgagcgata ttttagtcac aggtgcaagc ggctttattg gtaagttttt aatcttggca
       61 ttggttaaaa aaggccatcg ggtgtttgcc ttatttagaa aaccaacaga gcaatttcca
      121 cagttaatag catggctagc aacaaaaggc gtggctgagg cgcaaattgt gcctattcaa
      181 ggcgatttgt cagtcgctaa tgtgggcata agcgaagaaa attggcaaaa aatggccaat
      241 gtttcagtga tttatcatag cgcggcgatg tttggttgga atttatcaga gcagcaagct
      301 cgacaagtaa atgttaaagg tgtgcttgag ttattgacct tggcacaaca aaagttgtcc
      361 ttaaaacagg tggtacaagt atcaggttat atgttaacca tgactgaaca tttacaacaa
      421 ttgggtattg aaggtttggc cgaaaatgct aattggtcaa aaatctatca gcaagtaggc
      481 gtgtatgaag cctcaaaaat tgaggcacat tttgctatta aacgtttaag taaacagctt
      541 aatatacctt taacggtgat tcatcctgcc acagtgattg gtgatcatca acaaggggag
      601 ttggctagtc agcagccatt ttatcaaact ttagtcgatt taaaggcggg caagttattt
      661 gctgtacctt cggggcaggg ttaccgtttg ccattgggga gtgtagctta tttaacgcag
      721 tttatggcta atgttattga ttacccacaa acgataaatc aagaatatgt tttggctgat
      781 aattccacac caaacctaaa gcaagtatta acggtgtgtg ctaaggctat tgccattaaa
      841 ccacctatgt tgtcgattcc cattcctgtt ttaaaatgtg tgttgactag cgagtttgtc
      901 gctaaaaaag caggtatgag tttggagatg ttacattttt ttcgtcaaga atccttagcc
      961 acagaagcca cacaacaact ggccaatatg atggccttac ccttacctag tttggagcat
     1021 tgtttacagc aaacggcaaa gtttgtcgct aggcaatctt tggcaaaggc aagctaacag
     1081 taatttctaa accaccttct gggtggtttt taatatctaa taaacctttg tgcattttaa
     1141 caatacgcgc cacaatggct aaacctaatc ccgaaccttg tgtggtgcgt gccgattcgc
     1201 cacgaacaaa gggttgtaat agttcgggaa ttttactttc ttcaatgcct gtaccgtggt
     1261 cacgaacact aatcatcaag tattcacgat tataagtggc gttaagttct ataggggggt
     1321 gggcataacg taaagcatta ttgaccaagt taccgactaa acgtttaatc gcggtacgtc
     1381 taacggcaat caggggtagt tgtggcgttg gttcaaattc aatggcggtt tgttgtgaaa
     1441 attgggcggc tacttccgac aaaataccat taatgtcaca cggctcaagg gcttcatctg
     1501 aaccgtcacg cataaaggca ataaactgct ctaaaatcgc atccatgtct tcaatatcta
     1561 aaatcatgcc ttcggctaat tctttatcag acatcatttc agccgttaaa cgtaaacggg
     1621 tgaggggagt gcgtaaatca tgggaaatcc ctgctagcat taccgttcta tctttggcag
     1681 cctgttgaat ttcgagagtc atttgattaa aagcagcatt aactgctcta atttctgaag
     1741 ggccgcgttt gatttcaagc atggtggcgt gttggccttt agcaacccga cgagcagcta
     1801 attctaaacg ttttaatggt cgatttagtt gccgtaccaa aatcacaata gcaataattg
     1861 ccataattgg cacaccaatc acccaagcaa taataataaa tgggttgtat tgggcaaaaa
     1921 atagtaaagg ctcacgcacc cacacatctt tcatggaggg aatatacatc cacaagcgtg
     1981 gtgtgggttt aaattcaaaa tacacatcaa caggttcatg cagttcgcgg cgtaattgtt
     2041 cttctagttc ttgggtaaaa aattcggcaa agggtttgct tttgagtgtt ggaaattctt
     2101 caggattacg caccacctcg atgccagttg ctttttcaac ccatgcttgc atttcgaggg
     2161 cttgtggatt ttgtaataca tcatcttcgg cattacgcaa caaagacaaa ttaaccgcaa
     2221 cataatggcc gtgttggcgc atttctggca gatataaatt ccaccaaaaa aacgccaacg
     2281 ataagtattg acttactaaa agtacaatac taatcactaa ggttgtgcgc catactgcac
     2341 ttttgggttt aagtcttgcc caccaatagc tcatgattgt ttagttactc tttgccatct
     2401 ggtacaaaca catatccgac tccccaaacc gtttggatat aacgcgcttt ggtggagtct
     2461 tcttcaataa gtcggcgcaa acgagatact tgcacatcaa tagaacgctc cattgctccc
     2521 cattcacggc ctctcgctaa attcatcaac ttgtcacgag tgagcggttc acgtggatgt
     2581 tgcactaagg cttttaagac ggcaaattca cccgtagtga gtggcacatc ttcaccattt
     2641 ttacgaaatt gacgggtgga taaatctaat tcccattcac caaaactgac ggtttcgg
//
LOCUS       DAOQMB010000316         2695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22443_length_2695_cov_16.757576, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000316 DAOQMB010000000
VERSION     DAOQMB010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22443_length_2695_cov_16.757576"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLY05_09320"
     CDS             <1..722
                     /locus_tag="PLY05_09320"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNC03539.1"
                     /translation="KPKPIEKEPVAKPKATEEQVKAAKQQAQQQLASVADDLAALRDL
                     DVVATSKPATGGLQKDTGAAPAGTSRNMLTSKAGASSGGVAYGGPASSGFGGGVAGGS
                     GSKGNLGLGSKGTTDMSGKQIAAAGGSGSGEGGKTRGEGGIGKRTQEDIRKIFDQNGG
                     RLNNAYQRALKDDPSMEGTVRLKLTIDPSGKVTGCEVASSDLNNPELEAKIVSLVKGF
                     NFGADEVEVWKGTYPVNFYPN"
     gene            829..918
                     /locus_tag="PLY05_09325"
     tRNA            829..918
                     /locus_tag="PLY05_09325"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:863..865,aa:Ser,seq:tga)
     gene            1047..>2695
                     /locus_tag="PLY05_09330"
     CDS             1047..>2695
                     /locus_tag="PLY05_09330"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNC03540.1"
                     /translation="MSQTIVNAPAFVKHQKLIAWVAEIAALTQPDRIEWCDGSEEENQ
                     RLLDLMVSTGMMKKLNQEKRPNSYLASSDPSDVARVEERTFICSAKREDAGPTNNWED
                     PVVMRTKLNGLFEGCMRGRTMYVVPFSMGPLGSHIAQIGIELSDSPYVVVNMRIMARM
                     GKAVYDVLGEDGSYVPCVHTVGKPILTAADDVKWPCNPEKYIVHYPETREIWSFGSGY
                     GGNALLGKKCLALRIASVMGRDQGWLAEHMLILGVTNPQGEKHYVAAAFPSACGKTNF
                     AMLVPPAGFEGWKIETVGDDIAWIKPGTDGRLYAINPEAGFFGVAPGTNTTTNPNCMA
                     TLDKNVIFTNVALTDDGDVWWEGLTKTAPAHLIDWTGQDWTPDCGRKAAHPNSRFTVA
                     AAQCPSIDADWENPNGVPISAFIFGGRRGDTMPLVVEAADWQAGVYKAATMGSETTAA
                     AVGQQGVVRRDPFAMLPFCGYHVGDYFAHWLALGEKLQASGATLPRIFNVNWFRTDEN
                     GKFIWPGFGQNMRVLKWIIDRCENRAQGEETPIGIVPRYAD"
BASE COUNT          719 a          563 c          647 g          766 t
ORIGIN      
        1 ttaagccaaa gccaatcgaa aaagaacccg ttgctaaacc caaagcaact gaagaacaag
       61 tgaaagcggc taaacagcaa gctcagcaac aacttgcttc tgtggctgat gatttagctg
      121 ctttacgtga tctggacgtg gtagcaacct ctaaacccgc tacaggtggt ttgcaaaaag
      181 acactggtgc tgcacccgca ggcacaagtc gtaatatgct caccagtaaa gcaggagcta
      241 gttctggtgg tgttgcctac ggtggcccag caagctcagg ttttggtggt ggtgttgcag
      301 gtggtagtgg cagcaaaggt aatcttggct taggctctaa aggcacaacc gacatgagtg
      361 gcaaacaaat tgctgcggct ggtggttctg gcagtggtga aggtggtaaa acccgtggtg
      421 aaggtggtat tggcaaacgt actcaagaag atattcgtaa gatattcgac caaaatggcg
      481 gccgtttaaa taatgcctat caacgtgcat taaaagatga ccccagcatg gaaggtactg
      541 tacgcttaaa actgactatt gatccaagtg gtaaagtcac aggttgtgag gttgcttcaa
      601 gtgacttaaa taatcctgag ttggaagcca aaattgtctc tttagttaaa ggttttaact
      661 ttggtgcaga tgaggttgaa gtatggaaag gtacttatcc tgtcaacttc taccccaact
      721 aatgacataa aatgtcaaaa aaaggcgcgt aatgcgcctt tttttatcaa aaagtcttga
      781 gatttagtga cattttcaaa aaattagggt attcttcgcg gcctaatcgg aggcgtggcc
      841 gagcggttta aggcaccggt cttgaaaacc ggcgacggtt taccccgtcc gtgagttcga
      901 atctcaccac ctccgccaat ttttttcttg gtttccccaa tattggaaac caacttagct
      961 atcagcaaat cttattcttc ttttatacac tatcaggtca cccctgtagt gcacggatgt
     1021 ttttttagtt gattgggcct cacaccatga gtcaaacgat tgttaacgct cccgcttttg
     1081 ttaaacacca aaaacttatt gcttgggttg ctgaaattgc agctcttact caaccagacc
     1141 gcatcgaatg gtgtgatgga agcgaggaag aaaaccaacg cttattagac ctcatggtca
     1201 gcacagggat gatgaagaaa ttaaatcaag aaaaacgtcc caattcttat ttagctagct
     1261 ctgatccttc tgatgttgct cgtgttgaag agcgcacctt catttgctct gctaagcgtg
     1321 aagatgctgg cccaacaaac aactgggaag atccagttgt aatgcgtacc aaattaaatg
     1381 gcttatttga aggctgtatg cgtggtcgta ccatgtatgt tgtgcccttc tctatgggtc
     1441 cattaggcag ccatattgct caaattggta ttgaattatc cgacagccct tacgttgttg
     1501 tgaatatgcg tattatggcg cgtatgggca aggctgttta tgacgtattg ggtgaagacg
     1561 gcagttatgt gccttgcgta cacactgttg gtaagccaat tttaaccgct gctgacgatg
     1621 ttaaatggcc atgtaaccca gaaaaataca ttgtgcatta tccagagact cgcgagattt
     1681 ggtctttcgg ttctggttat ggtggaaatg ccttattggg caaaaaatgc ttagccttac
     1741 gtattgcttc tgtgatgggt cgcgatcaag gttggttagc tgaacatatg ttaattttgg
     1801 gcgttactaa cccacaaggc gaaaaacact acgttgctgc tgctttccca tctgcctgtg
     1861 gtaaaacaaa ctttgcgatg ttagtaccgc cagcaggttt tgaaggctgg aaaattgaaa
     1921 ctgtaggcga cgacattgct tggatcaaac ctggtactga tggtcgttta tacgcgatta
     1981 atccagaagc aggtttcttc ggtgttgcac ctggcacaaa caccacaacc aatccaaact
     2041 gtatggcaac attagataaa aacgttatct ttaccaacgt tgcattaaca gatgatggtg
     2101 atgtttggtg ggaaggttta accaaaactg ctccagcaca tttaattgac tggactggcc
     2161 aagattggac acctgactgt ggccgcaaag ctgctcatcc aaactctcgc ttcacggttg
     2221 ctgctgccca atgtccatct attgatgctg attgggaaaa tccaaacggt gtaccaatct
     2281 ctgcattcat tttcggcggt cgtcgtggcg acaccatgcc tttagtagtt gaagctgctg
     2341 attggcaagc gggtgtttat aaagccgcaa cgatgggctc tgaaaccact gctgctgctg
     2401 ttggccaaca aggtgttgtt cgtcgtgacc catttgcgat gttgcctttc tgtggttacc
     2461 acgtaggtga ttattttgct cactggttag ctttgggtga aaaattacag gcttctggcg
     2521 caactttacc acgtattttt aacgttaact ggttccgtac agacgaaaat ggcaagttta
     2581 tctggccagg ttttggtcaa aacatgcgcg ttttaaaatg gatcattgat cgttgtgaaa
     2641 atcgtgctca aggcgaagag acacctattg gtattgttcc tcgttatgcc gattt
//
LOCUS       DAOQMB010000317         2680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22665_length_2680_cov_15.955810, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000317 DAOQMB010000000
VERSION     DAOQMB010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22665_length_2680_cov_15.955810"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1281
                     /gene="nhaA"
                     /locus_tag="PLY05_09335"
     CDS             88..1281
                     /gene="nhaA"
                     /locus_tag="PLY05_09335"
                     /inference="COORDINATES: protein motif:HMM:NF007111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HNC03541.1"
                     /translation="MNQPYKPTTGLWVLTSLLKRFLQSESKAGIILILCTLTSLMLAN
                     SAVGESYTHFWHINIAGMSLEHFINDALMAIFFLLVGLEIERQIYVGELRQLKAALLP
                     IFAAIGGMCVPALIHYGFNGGTPYQDGFGIPMATDIAFALGVLLLLGNKVPVALKIFL
                     VALAIIDDLGAIIVIAIFYSKGIAWTYLAAALAIFAVLFVCNRMNVNRLSPYLVLGAV
                     AWYCMLHSGIHATITGVILAFLIPFTDGGRESPSYILEHKLTSIVAFAILPLFALANT
                     AIVIPETWADQLLTTHSLGIMLGLLLGKPIGVFLFSYLAIKLGWSTLPPEMNWKHVWG
                     VGLLAGIGFTMSMFITVLAFDSPELVISAKIAIMLGSLIAAVLGVIWLLVFAKHHEAE
                     QQHHV"
     gene            1274..1585
                     /locus_tag="PLY05_09340"
     CDS             1274..1585
                     /locus_tag="PLY05_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HNC03542.1"
                     /translation="MYKITFYVPITHVEQVKQAMFVAGAGRIGDYDCCAWQVLGVGQF
                     RPLVGAKPFIGQIDQIEQVAEYKVEMVCDEAYLSAVIKALKANHPYQQPAYDVTKNLD
                     I"
     gene            1679..2674
                     /locus_tag="PLY05_09345"
     CDS             1679..2674
                     /locus_tag="PLY05_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC03543.1"
                     /translation="MSERTDAAILLRFVYQAMRNAGLPAEQVLMGAGVGLNHLDANRR
                     TPSAAQLAFWQSAEKISKDPHIGLHLGEHLPLYRGQVLEYLFLSSSTFGEGLKRVLAY
                     QRLISDMLQAQLVINDDECYLANMLNDSTFRHTAECIMVAVLRFFRFVSEGQFQPLMI
                     YFTHEDGANAEEYERVYGCPVVLGADEIRLYFKPEVLKNRIWQAEPELLRFHEQLAHE
                     KLQELARFDLVAEVRRAIGESLESGTTSLETVAKRLNIAPRRLRSQLTEANTSFNQVL
                     ADYRCRLAKRLLAQTSESIEQIVYLTGFSEPSTFYRAFKRWTNLTPIEYRKKKQG"
BASE COUNT          761 a          445 c          587 g          887 t
ORIGIN      
        1 taatggatag tgaggaatct ggtcaatttt aaaattgtaa gtaatcattg aatttataat
       61 gattaatgaa aaatatggga gaaatacatg aatcaaccct ataaacccac tacaggacta
      121 tgggtcttaa cctcattatt aaagcgtttt ttacaaagcg aaagtaaagc aggcattatt
      181 ttaatcctgt gtacgctcac ttcattaatg ttagctaatt cagcagtagg tgagagttat
      241 acccattttt ggcatattaa cattgcaggc atgagtcttg agcattttat taatgatgca
      301 ctcatggcta ttttcttttt attagtcggt ttagaaattg aacggcagat ttatgtgggt
      361 gagttgcgtc aacttaaagc ggctttactc ccgatttttg cggcaatagg tggcatgtgt
      421 gttcctgcat taattcatta tggttttaat ggtggcacac cttatcaaga tggttttggt
      481 attcccatgg ctaccgatat tgcttttgcg ttaggcgtgt tgttgctgtt gggtaataaa
      541 gtacccgttg ccctaaaaat atttttagtc gctttagcga ttattgatga tttaggggca
      601 attattgtca ttgctatttt ttatagcaaa ggcattgcat ggacatattt agcggctgca
      661 ttagccattt ttgcagtgtt atttgtctgt aatcgcatga atgtgaatcg tttaagccct
      721 tatttagtat tgggggcggt ggcatggtat tgcatgctgc actcagggat tcacgcgacg
      781 attacaggtg taattttagc attcttgatt ccatttacag atggcggtcg tgagtcacct
      841 tcgtatattt tagaacataa actcacaagt atcgtagcgt ttgccatttt gcctttattt
      901 gctctcgcta ataccgcgat tgtcattcct gaaacatggg cagaccaatt attaacaaca
      961 catagtttag ggattatgtt gggcttatta ttaggcaagc ctattggcgt atttttgttt
     1021 agttatttag cgattaaatt gggatggtca acattgccgc cagaaatgaa ttggaaacac
     1081 gtatggggcg ttgggctatt ggcaggtatt ggttttacca tgtcgatgtt tattacggta
     1141 ttagcttttg atagtcctga actcgtcatt agtgccaaaa ttgccattat gttaggctct
     1201 ttgattgcgg cggttttagg agtgatttgg ttattagtct ttgctaaaca ccatgaagca
     1261 gagcaacaac atcatgtata aaatcacttt ttatgtgcct atcactcacg ttgaacaagt
     1321 caaacaagca atgtttgttg caggagcagg acggattggt gattatgatt gttgtgcatg
     1381 gcaagtgttg ggagtggggc agtttcgccc cttagtaggt gcaaaacctt ttattggcca
     1441 aatagaccaa attgagcaag tggctgaata taaagttgaa atggtttgtg atgaagctta
     1501 tttatcagca gtaataaaag cattaaaagc caatcatcct tatcaacaac ccgcttatga
     1561 tgtgacaaaa aatttggata tttagccaat agactgatag taattacttg attttataaa
     1621 aaataaggct ttactattag tcaatcaacc tttgtcccta cactaaagga gaacaataat
     1681 gtcagaacgt accgatgccg cgatattatt acgttttgtt taccaagcta tgcgtaatgc
     1741 aggcttacct gctgagcaag tgttaatggg ggcaggcgtt ggtttaaatc atttagatgc
     1801 aaatcgtcgc acgccttcgg cggcacaatt ggcgttttgg caatctgccg aaaaaatttc
     1861 taaagaccca cacattggtt tacatttagg tgagcattta cctttgtata gagggcaagt
     1921 attagagtat ttgtttttaa gtagctcaac ctttggtgaa ggcttaaaac gagtcttagc
     1981 gtatcaacgt ttaattagcg atatgttaca agcccaatta gttattaatg acgatgaatg
     2041 ttatttggcg aatatgctca atgattcgac ctttaggcat acggctgaat gtattatggt
     2101 agcagtgttg cgcttttttc gttttgtttc cgaaggccaa tttcagccat taatgattta
     2161 ttttacccat gaagatgggg caaatgccga agagtatgag cgtgtttatg gttgccctgt
     2221 ggtattaggg gcagatgaaa tccgtttata ttttaagccc gaagtgttaa aaaatcgtat
     2281 ttggcaagca gaaccagaat tattaagatt tcacgagcaa ttagctcacg aaaaactcca
     2341 agagttagcc cgttttgatt tggttgcaga agtaagacgt gctattggtg agtctttaga
     2401 aagtggcaca actagtcttg aaacggttgc taaacgttta aatattgcac cacggcgatt
     2461 acgcagccaa ttaaccgaag ccaataccag ttttaaccaa gtattagcgg attatcgttg
     2521 tcgtttggcc aaacgtttgt tagcacaaac cagtgaaagt attgaacaaa ttgtctattt
     2581 aacgggattt tcagagccaa gcacttttta tcgtgccttt aagcggtgga cgaatttaac
     2641 gcccattgag tatcgtaaaa agaaacaagg ctaatcattt
//
LOCUS       DAOQMB010000318         2679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22680_length_2679_cov_26.678735, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000318 DAOQMB010000000
VERSION     DAOQMB010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22680_length_2679_cov_26.678735"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2679
                     /locus_tag="PLY05_09350"
     CDS             <1..>2679
                     /locus_tag="PLY05_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNC03544.1"
                     /translation="AQFTFQTFDDVKGRQDKTLVRIYHGTFNEHKAKLAELNKRGGGV
                     FVTINETDLRGRSLANMLKVRALFVDLDGSPLQPILDLPEDLHPHIIIESSPDKWHCY
                     WLVNNCELEQFKPLQQALAAKFDGDKQVCDLPRVMRLAGFSHNKGESFITRIHTMQDN
                     LYPYSVNKLIVGLGLNTNVSHRDIFPNNTFNTIDEEDFTIGLPPEADVIADLRSALEY
                     LPCEDYHDWIRQAMHLKTLGNEGLDLWLAWSSKSAKFDRAEALNKWHSLQANRTDYKA
                     IFNEAKAKGWINPLSNQSAAYELPDPLPITSSFAPVMPFSADLLPTALQEFVYDEADR
                     MPAPVSFVAVSVLVALGSVIGASCGIKPKQKDDWLIVPNLWGGIVAPPTSKKTPAIDS
                     GMRPLNFLINKARESYESDLKSHEKDKMLYKSKLEGLEADLKSASKTSSKNKPQKTRE
                     ELAELICQERENEPMPPCLRRYKTNDCTVPKLGELECANPNGILVLRDELIGLLAGLD
                     KEGKEEDRAFYLEGFNGTGSYDTDRIMRGSLFIKNHCLSVFGGIQPDKLIAYLEQAYS
                     GLGNDGLLQRFQMLVYPDSIEWQYRDRYPNREAANVVFDIFKKLSETDFIEFGAYPTD
                     EYNKRPYFRFSLDAQAFYVDWTHKLNTQTIPNEENTVIQQHLGKYERLLPALALIFHL
                     IDCASIGQANGFGVSLEAIQRAARWCEYLETHARRVYGLIDGMGMRPALTLSKKIEAL
                     IKRTAKTDETPENWLKDGFTLRDVRRKQWQHLKDDNAIEKALTVLADNYWIVPNDTHN
                     SKNGGRPTTRYFISQKLKTSIPPTAKTDETQKMNNFSEKTAQKIGFGSFGSTHLGDFE
                     NLENDSLILTKNELDDDFKTPIDPNDKKVSCSMCEH"
BASE COUNT          870 a          540 c          552 g          717 t
ORIGIN      
        1 acgctcaatt cacattccag acctttgatg atgtcaaagg ccgtcaagac aaaacacttg
       61 tccgtattta tcatggcaca tttaacgaac acaaagccaa acttgccgaa cttaacaaac
      121 gcggtggcgg tgtatttgtc acgattaacg aaaccgacct aagaggccgt agccttgcta
      181 atatgcttaa agtgcgagcg ttatttgttg acttagacgg ttcaccactt caaccgattt
      241 tagacctacc cgaagaccta cacccccata tcattatcga gtctagccct gataaatggc
      301 attgctactg gcttgtaaac aattgcgagc tagagcaatt taagccatta cagcaagcac
      361 ttgccgctaa gtttgatggt gataaacaag tatgtgactt gccgcgcgtg atgcgtttag
      421 caggttttag ccataacaaa ggcgaatcat ttataacgcg cattcatacc atgcaagata
      481 acctttaccc ctacagcgtg aataagttaa ttgttggttt aggcttaaat actaatgtct
      541 cgcatagaga tatatttcct aacaatactt ttaatactat tgatgaagag gattttacga
      601 tagggctacc acccgaagcc gatgtcatag ccgacctaag aagcgcactt gagtatttac
      661 cttgcgagga ttatcacgac tggataagac aagccatgca cctaaaaacg ctaggcaatg
      721 agggcttaga cctatggctt gcatggtcaa gcaaaagtgc aaagtttgat agagccgaag
      781 cccttaacaa atggcactca ttacaagcca atagaacaga ctacaaggcc atttttaacg
      841 aggctaaagc taaagggtgg ataaacccat taagtaatca aagcgcggct tatgagctac
      901 ctgacccctt acctattaca agcagctttg cccctgtcat gccgtttagt gctgatttat
      961 tgcctactgc attgcaagag tttgtatatg atgaagcaga cagaatgcct gcccccgtgt
     1021 catttgttgc ggtatctgtt ttagtagcat tgggaagtgt aattggtgca agttgtggta
     1081 tcaaaccaaa acaaaaagat gattggttaa ttgttcctaa tttatggggt ggcattgttg
     1141 caccgcccac ttctaaaaaa acccctgcta ttgactcagg aatgcgccca cttaactttt
     1201 taatcaacaa agcacgcgaa tcttatgaga gcgacttaaa aagccatgaa aaggataaaa
     1261 tgctttataa gtcaaaatta gagggcttag aagccgacct aaaaagcgcg tctaaaacat
     1321 catcaaaaaa taagcctcaa aaaacacgcg aagaattagc agaactcatt tgccaagaaa
     1381 gagaaaacga gcctatgccg ccctgtttaa gacgttataa aaccaatgat tgcaccgtac
     1441 ccaagttagg ggaattagag tgtgcaaacc ctaatggcat tcttgttttg cgtgatgaat
     1501 tgataggctt gcttgcaggt ttggataaag agggcaaaga agaagaccgc gctttttatt
     1561 tagagggctt taatggtact ggcagttacg acacagacag aataatgaga ggaagtcttt
     1621 ttattaaaaa tcactgttta tcagtgtttg gaggcataca acccgataaa ctaatcgctt
     1681 atttagagca agcgtacagt ggtttaggca atgatggctt actgcaacga ttccaaatgc
     1741 ttgtctatcc tgattctatc gagtggcagt accgagaccg ataccctaat cgtgaggccg
     1801 ccaatgttgt ttttgatata ttcaaaaaac tgtcagaaac cgattttata gagtttggcg
     1861 catacccgac agatgaatat aacaaacgtc cttactttcg tttttcttta gatgcacagg
     1921 cgttttatgt tgactggact cataaattaa acacgcaaac gatacctaat gaagaaaata
     1981 cggttattca acaacactta ggcaagtatg agcgtttgtt acctgcatta gcgttaatct
     2041 ttcacctgat tgattgtgca agtattggcc aagccaatgg ttttggcgta agtcttgaag
     2101 caatccaaag agccgcgcgt tggtgcgagt atttagaaac tcatgcaagg cgcgtttatg
     2161 gcttgattga tggtatggga atgcgccccg ccttgacgct ttctaaaaaa atagaagcac
     2221 tcataaaacg tactgccaaa actgacgaaa cccccgaaaa ttggttaaaa gatggcttta
     2281 ccttgcgaga tgttagacgc aaacaatggc aacacttgaa agacgataat gctatcgaaa
     2341 aggcattaac ggtacttgct gataattatt ggattgtccc aaatgacacg cacaattcaa
     2401 aaaatggagg aagaccaaca acacgctatt ttatttctca aaaattgaaa acatctatac
     2461 cacctactgc caaaactgac gaaactcaaa aaatgaacaa ttttagcgaa aaaactgctc
     2521 aaaaaatagg ttttggcagt tttggcagta cccatctagg ggactttgaa aatttagaaa
     2581 atgattctct gattttgaca aaaaatgaat tggatgatga ttttaaaaca cctattgacc
     2641 caaatgacaa aaaggtgagt tgtagtatgt gcgagcatt
//
LOCUS       DAOQMB010000319         2667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22892_length_2667_cov_15.263783, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000319 DAOQMB010000000
VERSION     DAOQMB010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22892_length_2667_cov_15.263783"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="PLY05_09355"
     CDS             complement(<1..1065)
                     /locus_tag="PLY05_09355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNC03545.1"
                     /translation="MSSLAPVLFSELASTNGKKIAIAQLNSEKTLNSLTLPMIELLYP
                     QLQQWAKDDNVVAVVLQGAGEKAFCAGGDVKKICVAVREHGIDDKTAVDFFSAEYRLD
                     YLIHQFAKPFIVWATGFVMGGGIGLLAGASHRIVTETSRLAMPEITIGLYPDVGGSWF
                     LARLGKVGLFLGMTGAQINAADALQTGFASHALPSSARQQVLEQLALIEWAKDRQQHP
                     QQISQLLDQLALKELPQGQLALAQTDIEQRTQANNLLELYQQLIQPSSNAWLDKAATT
                     LKRGSPTSAALIYRQWQQGSTQSLADCFRQELTLSVQCARHPDFIEGVRALLVDKDQH
                     PQWQPATIEQVTEAWLDGHYQ"
     gene            complement(1062..1793)
                     /gene="ung"
                     /locus_tag="PLY05_09360"
     CDS             complement(1062..1793)
                     /gene="ung"
                     /locus_tag="PLY05_09360"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005304723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HNC03546.1"
                     /translation="MNTAVSVSLNSQEQEALARVQLDESWKAALASTFLQPYMANLRE
                     FLAEQRKLGKIIYPPAGEYFNALNTTPLSQVKVVILGQDPYHGVGQAHGLCFSVRRGV
                     SVPPSLQNIYRELFNDVGAQPVRHGDLSSWAAQGVLLLNSVLTVEAGQPASHQGRGWE
                     LFTDEIIKVLNEQCENLVFLLWGSYAQKKGQHIDTNKHLVLKATHPSPMAANRGGFFG
                     CKHFSQTNAYLAEHGLEPINWALPA"
     gene            complement(1833..2528)
                     /locus_tag="PLY05_09365"
     CDS             complement(1833..2528)
                     /locus_tag="PLY05_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HNC03547.1"
                     /translation="MSAMSSKSAKASYHGRKPARQGSEQRRLAILQAALRVMVRDGVR
                     GVRHRAVAQEADVPLSATTYYFKDIHDLLADALTLYAKDVMENFVNPFWVRIHEWLLV
                     NFPLYSGTDLKAVLPEVIEHMVDVAIDEILDRLKNHRDKLLTEQAFWYAVLQDERLHP
                     LALDYSQSVLRDITKMLELIGVEQAQNAGRLILMTIRHIEIQGLLQPVDSYDREVMKQ
                     LLLLQVRSVCHIN"
BASE COUNT          830 a          608 c          476 g          753 t
ORIGIN      
        1 ttgataatgg ccatctaacc atgcttcggt cacttgctca atggttgcag gttgccattg
       61 tggatgttga tctttatcga ccaataaggc acgtacacct tcaataaaat ctggatgacg
      121 cgcacattgc accgataaag ttaattcttg tctaaaacaa tcggccaaac tttgtgtgct
      181 gccctgctgc cactgacgat aaattaacgc agcactggtt ggtgaaccac gctttaaggt
      241 ggttgctgct ttatcaagcc aagcattgct actcggctga atcagttgtt gataaagctc
      301 tagcaaatta ttagcttggg tacgttgttc aatatccgtt tgagctaaag ctaattgacc
      361 ttgaggcagt tctttaagag ccaattgatc taagagttgg ctaatttgtt gtggatgttg
      421 ttgtctatct ttcgcccatt caatcaaggc taattgttca agcacttgtt gacgtgccga
      481 gcttggcaag gcatggctag caaacccagt ttgtagggca tccgcagcat taatttgcgc
      541 gccagtcata cccaaaaaca aacccacttt gcctaaacgt gccaaaaacc acgagccacc
      601 gacatcagga tacaaaccaa tggttatttc gggcatcgcc aaacgggatg tttcagtaac
      661 aatacggtgt gatgcccctg ccaacaagcc gatgccacca cccataacaa aacctgtcgc
      721 ccaaacaata aacggcttgg caaattgatg aatcaagtaa tctaaacgat attcagcact
      781 aaaaaaatca actgccgttt tatcatcaat gccatgttca cgtactgcga cacaaatctt
      841 tttaacatca ccacctgcac aaaaggcttt ttcacctgcc ccctgtaaaa cgaccgccac
      901 cacattgtca tcttttgccc attgttgcag ttgtggatac aacaactcaa tcataggtaa
      961 agttaatgag tttaaggttt tttcactatt taattgcgca atggcaattt ttttaccatt
     1021 ggtggaggct aattcactaa ataaaacagg tgctaaagaa ctcatgcagg taatgcccaa
     1081 ttaattggct ctaaaccatg ttctgctaaa tacgcattgg tctgagaaaa atgtttgcaa
     1141 ccaaaaaaac caccacgatt ggcagccatt ggcgatggat gggtcgcttt tagcactaaa
     1201 tgcttattgg tatcaatgtg ttgccccttc ttttgggcat aacttcccca aagcaagaag
     1261 actaaatttt cacattgctc atttaatact ttaataattt catcagtaaa taattcccaa
     1321 ccacgacctt gatgtgaggc aggttggcca gcttctactg tcagtacact gttaagtaat
     1381 aacacccctt gtgccgccca tgagcttaaa tcgccatgac gaacaggctg cgcgcccaca
     1441 tcattaaaca actcacgata aatattttgc aaagatggag gaacagacac accacgacgc
     1501 acagaaaaac acaaaccatg ggcttggcca acaccgtgat atgggtcttg tcctaaaatc
     1561 accactttga cttgtgacaa gggtgtagtg tttaaggcat taaaatattc gcctgcgggg
     1621 ggataaataa tcttacctag ttttctttgc tcagctaaaa actcacgcaa attagccata
     1681 taaggttgca aaaatgtgga agctaaggct gctttccatg actcatctaa ttgaactctg
     1741 gctaatgcct cttgttcttg gctgtttaaa gatacagata cggctgtatt catgctcaaa
     1801 tatccttaaa aattataaaa atacgctttt tcctaattaa tatgacaaac agaacgtact
     1861 tgtaataaca ataactgttt catcacttca cggtcatagc tatctacagg ctgcaataaa
     1921 ccttgtattt ctatatgacg aatggtcatt aaaattaaac gacctgcatt ttgtgcttgc
     1981 tcaacaccaa tcaactctag cattttggta atatcacgca acacactttg actataatct
     2041 aaggctaaag gatgcaaacg ctcatcttgt aacacggcat accaaaaagc ttgttccgtt
     2101 agcaatttat cccgatgatt ttttaagcga tctaatatct catcaatcgc cacatcaacc
     2161 atatgttcaa tcacttcggg caacacagct tttaaatctg tgcctgaata tagtggaaaa
     2221 ttaactaata accattcatg aatacgcacc caaaatggat taacaaagtt ttccatcaca
     2281 tctttggcat ataaggttaa agcatcagcc aataaatcat gaatatcctt aaaataataa
     2341 gttgttgctg acaacggtac atcagcttct tgagcaactg cccgatggcg tacaccacgc
     2401 actccatcac gcaccatgac ccgcaaagcg gcttgtaaaa tcgctaaacg acgctgttca
     2461 ctgccctgac gtgcaggttt tcgaccatga taagaagctt tcgctgattt agatgacatt
     2521 gcagacatga aaacaacact actcaaagaa aatctatata aaatcttatt atctattcta
     2581 acgaaatagt ttatcgtttg atatgaaata cacgccaatg ttgacagcaa acaccctact
     2641 ttgagtacaa ttggcgtatt agctgta
//
LOCUS       DAOQMB010000320         2666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_22911_length_2666_cov_27.911145, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000320 DAOQMB010000000
VERSION     DAOQMB010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22911_length_2666_cov_27.911145"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..1298
                     /locus_tag="PLY05_09370"
     CDS             213..1298
                     /locus_tag="PLY05_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HNC03548.1"
                     /translation="MENDQEKSPVSVTSLAAEETLDKARNSEVLDFPHRIERYGRARK
                     RALQMFYYLGAYQPPLPPLPPLLSPDKLPKQTESQLIKKSVARLKECGNWLHFRNYYT
                     VDNIRLINAVFCMQHLICPLCAIRRGAKYLSAYLKKYQQIKTEYPDLKPCIITFTVKN
                     HEDLVKVFSHLKDSLKTIIDTRRKYLSNPLRNRYTELSKVQGAVYTFELTNIGNGWHP
                     HVHMIALCSEIPIEAQIRKEWKSITGDSYIVDVTPFRTPKNYIHDNMYTDIQGYEVEA
                     FTEVFKYAVKFSDLSVQDNWYAYTKLKGQRLMGSFGLFRAVEVSEELTDELLEEELPY
                     IDLLYRFVRGHYNLANIIHPTDETSDK"
     gene            1851..2315
                     /locus_tag="PLY05_09375"
     CDS             1851..2315
                     /locus_tag="PLY05_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03549.1"
                     /translation="MSHYLIIHWSSVMNSISKSNEPSNLDMFMAQVTGTGETDFLGTE
                     HQISLRVNGHIHAQITAIMGIYDMHAREKGRRAPSRNNVLNDLLSIALEAVYSRLGDD
                     DRQCFDQLFSPACSGALRYVEEEEMEDFKTEMRHQAKKHEAAKNSKASKGDE"
     gene            2318..2641
                     /locus_tag="PLY05_09380"
     CDS             2318..2641
                     /locus_tag="PLY05_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017519115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03550.1"
                     /translation="MSLANALPDSPMFLLVGRLLHVFKTPSGTKKTGEAYEGQDKVQI
                     LGNIPLPDGGHRLDLVTLTTKDAALFEPFVGEVISCPIGCYAFNKTVGYFVPTGGKPV
                     VYCDK"
BASE COUNT          814 a          481 c          577 g          794 t
ORIGIN      
        1 ggtaaacctg tcgtctattg cgataaatag aggcattacg gaggggtttt ttttaggggt
       61 tgatgttcag acgtaaggtc gaacgcaagc cctaaaaatg cccctctttt gggatgtttg
      121 tcagtttgcg aagccttacc ttgatactat acaactaaag cgtcgggaac gtataaaaaa
      181 tgaacaattt gactaaaaag tgagcttttt tgatggaaaa tgaccaagaa aaaagccctg
      241 tcagtgttac cagcttggcg gcagaagaaa cactagacaa ggctcgaaat tccgaagttt
      301 tagacttccc ccatagaata gaacgatacg ggagagcgcg caagcgtgcc ttgcaaatgt
      361 tttactattt gggtgcttat cagccgcctt tacccccttt accgcctctt ttaagccctg
      421 ataaattgcc taaacagacg gaaagccaat tgattaaaaa atcagttgct cgtcttaaag
      481 agtgtggtaa ttggttgcat tttagaaatt attatacggt tgataatatt agattgataa
      541 atgctgtttt ttgtatgcag catcttattt gccctctttg tgctatccgt agaggtgcaa
      601 aatacctgtc tgcttatctt aaaaagtacc aacaaataaa gactgagtat cccgatttaa
      661 agccctgtat tattactttt actgtcaaaa accatgagga tttagttaag gtttttagtc
      721 atctaaaaga ttctttaaaa accattattg atactcgtcg aaaatatttg tccaatcctt
      781 taaggaatag atataccgaa ctgtcaaaag tacaaggtgc ggtttatacc tttgaactta
      841 ccaatattgg taatggttgg catcctcatg tccacatgat agccctttgc tctgagattc
      901 ctatagaggc tcaaattcgt aaagaatgga agtctattac tggcgatagc tatatcgttg
      961 atgtaacccc ctttagaact cctaaaaatt atatacatga caacatgtat acagatatac
     1021 aaggatatga ggttgaagct tttacagagg tatttaagta cgctgtaaag tttagtgatt
     1081 tgtcagtaca agacaattgg tatgcctata ccaagctaaa aggtcaacgt cttatgggta
     1141 gttttgggct ttttagagct gttgaggtat ctgaagaact aacggatgag ctactggagg
     1201 aggaactccc ctatatagat ttactctata ggtttgtcag gggtcactat aaccttgcga
     1261 atatcattca tccaaccgac gagacaagcg acaagtagaa ccaacaaagg ggggcacccg
     1321 cgcgcggggg gatgcctttt ttgcgttcga ccttgtgctt gtccaagcgt aggacgatgg
     1381 gaggcggata aggaggagga gacgacgacg cagacaacga ccagaggaca agcgtcaaaa
     1441 aacacgtttg ctacgaaatg taaaagagaa atcatatttt ttgctatgaa atgtattgag
     1501 attcctcttg ttatcactgc catagggttg gggggtcttg ggggaatcat tcccccaagt
     1561 cgggggggct ggggggcgaa gcccctccag tattaggcgc gaaatgtatt ttttaaataa
     1621 ctctacctat aaacgcgccg aaacgtgagc gaagcgaaca cggaaagaaa aacagactaa
     1681 taaaaaactc aatcttattt gtggccaaga tatgcgccaa cctaaagcga taacccaccc
     1741 ctaattaacc aaaaacaatt gattgcctaa gatgatttag ccgcgcgtat gcgcgtaatt
     1801 agcttgcttt tttttatatt ttgtttttat attaaataat gattctttaa atgagtcatt
     1861 atttaataat tcactggagt agtgttatga actcaatttc taaatcaaat gaaccatcta
     1921 atcttgatat gtttatggct caagttacag gtactggtga gactgatttt ttaggtacag
     1981 agcatcaaat ttctttgcgt gttaatggtc atattcatgc tcaaataaca gcaattatgg
     2041 gcatttatga tatgcatgct cgtgaaaaag gtcgtagggc tcctagcaga aacaatgttt
     2101 taaatgattt gctttcgatt gctttagagg ctgtttattc tcgcttaggc gatgatgata
     2161 ggcagtgttt tgaccaatta ttttctcctg cctgttctgg tgctcttcga tatgttgaag
     2221 aggaagaaat ggaagatttt aaaacagaaa tgagacatca agccaaaaag catgaagctg
     2281 ctaaaaatag taaagcatct aaaggagatg aataaaaatg tctttagcta atgcgttacc
     2341 cgatagcccc atgtttttat tagttggtcg tttacttcat gtttttaaaa ctccatcagg
     2401 aactaaaaaa acaggtgaag catacgaagg ccaagataaa gttcaaatct taggaaacat
     2461 tccgcttcca gatggtggtc accgtctgga cttagttacc ttaacaacga aagatgcggc
     2521 cttatttgag ccatttgttg gcgaggtcat aagctgtcca attggctgct acgcgtttaa
     2581 caagactgta gggtactttg tccctactgg tggtaaacct gtcgtctatt gcgataaata
     2641 gaggcattac ggaggggttt ttttta
//
LOCUS       DAOQMB010000321         2658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23057_length_2658_cov_16.954284, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000321 DAOQMB010000000
VERSION     DAOQMB010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23057_length_2658_cov_16.954284"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /gene="earP"
                     /locus_tag="PLY05_09385"
     CDS             <1..1009
                     /gene="earP"
                     /locus_tag="PLY05_09385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HNC03551.1"
                     /translation="LQVRLWVDDLASFAKLESAIQVNLDQQHYLGIDIRYWTAVAFIN
                     SPTPHEMVIEGFGCRLPDSFLQAMAQQSPPPKWLNLEYLSAEAWTLDCHGLNSIHPTL
                     ALKQQFFFPSFEVRGGGLLRERDLIQQRDAFNADLQKQFWQQLGYSQATDADYRLSLF
                     AYENLAAPALLQTLSQQLARGFIAIPQGRIVHSLETWLNKPLKVGDCVQVGSISLAIL
                     PFLSSSDYDHLLWACDINCVRGEDSFIRAHWAAKPLLWHIYPQDEDAHLDKLAAWQQL
                     SQAVIDQEWQQLQRAWVKQDTNPEIWQTLLSTAQANTKGHQQFCDYLTKQDDLCQRLL
                     A"
     gene            1141..1707
                     /gene="efp"
                     /locus_tag="PLY05_09390"
     CDS             1141..1707
                     /gene="efp"
                     /locus_tag="PLY05_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNC03552.1"
                     /translation="MKTAQELRAGNVFMVGTEPMVVVKAEYNKSGRNAAVVKMKLRNL
                     LNGQQTETVYKADEKFEPVFLESKDVTFSYFADPMYIFVDADYEQYEVEKENLGDAVN
                     FIEDGMEETCQAVFYNGKAISVELPTTIVRKVAYTEPAARGDTSGKVMKQAKLANGYE
                     LQVAAFIETDERIEIDTRTGEFKSRAKG"
     gene            complement(1747..2598)
                     /locus_tag="PLY05_09395"
     CDS             complement(1747..2598)
                     /locus_tag="PLY05_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03553.1"
                     /translation="MLSISRYVSIMMTGMNLLPTIGWAAEQMVLTREDQRPVTVVKYI
                     PQQSCQGVAIISHGAGGSEYGYSYLANFLQQQQWLVLVVDHQESNSEVLKQLLRRQGM
                     IEALTELVTTASAYQGRFMDISMAQAWAKQHCAGSLQVLLGHSMGAATTMLLAGAENN
                     LNVLAPKLSFDAYIALSPQGVGSIFPKDAWRHINKPVLLLTGTKDRELHGSWQSRQDA
                     FYSLPAGCKWLGVINGANHLNFAGKGASGKTERLVTQTVAEFIEGVKQKQCQPSLKLD
                     DLTLKSK"
BASE COUNT          793 a          592 c          511 g          762 t
ORIGIN      
        1 cctacaggtt cggctgtggg tggatgattt ggccagtttt gccaaattag aatctgctat
       61 tcaagttaat ctagatcaac aacattattt aggcatagac attagatatt ggacagcagt
      121 ggcttttatt aactcgccta cacctcatga gatggtgatt gaagggtttg gctgtcgttt
      181 acccgatagc tttttacaag caatggctca acaatcaccg ccacccaaat ggctaaattt
      241 agaatatctc tctgccgaag cttggacatt ggattgtcat ggtcttaact ctattcatcc
      301 aacgttggca ctcaaacaac agtttttctt tcctagcttt gaagtgcgtg gtggtggctt
      361 attacgagaa cgtgatttaa ttcaacaacg cgatgcgttt aacgcggatt tacaaaaaca
      421 attttggcaa caactcggct atagccaagc cacggatgcc gattatcgtc tgtcattgtt
      481 tgcttatgaa aacttggctg cgcctgccct acttcaaact cttagccaac aattagcacg
      541 aggctttatt gccattccac aaggccgaat tgtacatagc ttagagactt ggctgaacaa
      601 acccttaaaa gttggcgatt gtgtgcaagt tggctcaatc agcttagcga ttttaccgtt
      661 tttatccagc agtgattatg accatttatt atgggcgtgt gacattaatt gtgtgcgtgg
      721 tgaagactca tttattagag cgcattgggc agccaaaccg ttgttatggc atatttatcc
      781 ccaagatgaa gatgcccacc tagacaaact agcggcttgg caacaactca gccaagcggt
      841 tattgaccaa gaatggcaac aactacaacg agcatgggtt aaacaagaca caaatcctga
      901 aatttggcag acattgttaa gcaccgctca agccaataca aaaggtcatc aacaattttg
      961 tgactattta actaaacaag atgatttatg ccagcgttta cttgcctaaa atatttaagg
     1021 caaaggatat tgatgaatgt cttttgctta aatagcagcc tttagggtag aatatggcac
     1081 ttttttaggg tttagaatgt tttttaaacc tagtctcaat ttttgctgtg tgataatccc
     1141 atgaaaaccg ctcaagaact ccgtgctggt aatgtattta tggttggcac tgagccaatg
     1201 gttgttgtaa aagccgaata caacaaatct ggccgtaatg ctgccgttgt aaaaatgaaa
     1261 ttacgtaact tattaaacgg tcaacaaact gaaaccgttt ataaagccga cgaaaaattt
     1321 gaacctgtat tcttagaaag caaagacgtg actttctctt actttgctga ccccatgtac
     1381 attttcgtag acgctgacta cgaacaatac gaagttgaaa aagaaaactt aggtgatgcg
     1441 gttaacttta tcgaagacgg tatggaagaa acttgccaag ccgtattcta taacggtaaa
     1501 gcgatttctg tagaattgcc aacgactatc gtgcgtaaag ttgcttacac cgagccagct
     1561 gctcgtggcg atacttctgg taaagtcatg aaacaagcta aattagctaa tggttatgaa
     1621 ttacaagttg ctgcatttat cgaaaccgat gagcgcatcg aaattgatac tcgcactggc
     1681 gaattcaaat ctcgtgctaa aggctaattt agcacaagtt taaaaaaggc gcgtgatgcg
     1741 ccttttttat ttacttttta gagttaaatc atctagtttc aaggaaggct ggcactgctt
     1801 ttgtttaaca ccttcaataa attcggcaac tgtttgagtg actaaacgtt ctgtcttgcc
     1861 cgatgctccc ttccctgcaa aatttaaatg atttgcacca ttaatcacgc ccaaccattt
     1921 acaacctgcg ggcaaactat aaaacgcatc ttggcggctt tgccaagagc catgcagctc
     1981 tcgatcttta gtgcctgtta acaacaaaac tggcttatta atatgacgcc acgcatcctt
     2041 gggaaaaata ctgcccacac cttgtggcga taaagcaata taagcatcaa acgacaattt
     2101 aggggcaagc acatttaaat tattttctgc tcctgccaat aacatcgtcg tcgctgcgcc
     2161 catcgaatgc cccaacaaca cttgtaatga acctgcgcaa tgttgttttg cccatgcctg
     2221 cgccatacta atatccataa atcgcccttg ataggcgctt gcggttgtca ccaactcagt
     2281 taatgcctca atcatccctt gacggcgtaa taattgtttt aatacctcac tattactttc
     2341 ttgatggtca actaccaaca ccaaccattg ttgctgttgt agaaaattgg ctaaatagct
     2401 atagccatat tctgaaccac ctgcaccatg agaaataatc gcgacaccct gacaactctg
     2461 ttgggggata tatttcacca ccgtcacagg acgctgatct tctcgagtca acaccatctg
     2521 ctcagccgcc caacctatcg ttggtaaaag attcatccct gtcatcataa tagacacata
     2581 acggctaatg cttaacataa gaatccctat ctataaaaag cttaaaacta agccaattaa
     2641 accacccacc aacgtacc
//
LOCUS       DAOQMB010000322         2654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23110_length_2654_cov_14.894960, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000322 DAOQMB010000000
VERSION     DAOQMB010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23110_length_2654_cov_14.894960"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLY05_09400"
     CDS             <1..518
                     /locus_tag="PLY05_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03554.1"
                     /translation="KRIPMMFIFGSAEQIAQSIYPILAISTLLTLLVCLLVYKVFGIS
                     KWIMVALAVLLLTATATFSTFNLSTMFYQLSLQGQQVTITLAVPDVYRQSFQAHQLKY
                     VDAVTPNPKYDLCHVVLEDTQGMLYQSLDTDVKRCEDIQKTLVQALQLKARPPKPKSS
                     HSPPPLPSHVK"
     gene            complement(524..1531)
                     /locus_tag="PLY05_09405"
     CDS             complement(524..1531)
                     /locus_tag="PLY05_09405"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HNC03555.1"
                     /translation="MQGVYVASDLANLLRAYLDKYQIDAPSIRHQLAAWPPHAQMPMK
                     VWWQLLEEMQTLLHEPALGVKIGQCVQPQHTGVLAYLIMHCATLGEALLHFQRFQKLL
                     HNMSDVLLTSHQQALKLSWDLSLGASTQLSDEVFMSGLITFVKQMVQGMGLQPVALHF
                     MHPAPCDPQLYQELIGCPVFFNAKQVSIDIPLEALSLTINSQNPYLLELLAKQAEALI
                     DQSTHDDLLLETIRRFCAERLHQHVPTLDEVASTLNLSTRTLHRRLADRGLNFHHLLA
                     ETRFRLAKHYLSDPRLQLNQIALLLGYSEQSAFTRAFSAWAGISPQRFRNRSPFGRGL
                     G"
     gene            1665..2552
                     /locus_tag="PLY05_09410"
     CDS             1665..2552
                     /locus_tag="PLY05_09410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HNC03556.1"
                     /translation="MSDVVHALMFIVLFGGLFISVIAAEAYYWHKKGRPEMYSFKETI
                     TNMMMGASYKIVDGIAVALFIRVFFDWVYQFGFQYKPVHGIFSLILIFLVVDASFYLC
                     HFMMHKVRWFWTSHVTHHSAERMNYSTALRQNFTMVFNGAWLFWWVPVALVGFDKNWV
                     TIAIELNLAYQFFLHTETRSPLDKFGFILNTPSHHRVHHGSQPAQIDTNFGGVLIIWD
                     KLFGTFVPEEKAGKITYGLTERQPHSLNPFFLQVHEWADMFKDFKASGYDFRVFVKGP
                     GWKSDKISTENNKHKPLAN"
BASE COUNT          801 a          545 c          498 g          810 t
ORIGIN      
        1 ttaagagaat ccccatgatg tttatttttg gtagtgctga acaaattgcc caatctattt
       61 atcccatttt ggcaattagt acgctcttaa cactcttagt gtgtttattg gtttataaag
      121 tttttggcat ttctaaatgg attatggttg ctttagcggt gttgttatta accgcaacag
      181 caacatttag cacctttaat ttaagcacca tgttttatca actcagctta caaggccaac
      241 aagtcactat tactttagct gtgcctgatg tttatcgaca aagctttcaa gcacaccaat
      301 taaaatatgt tgatgctgtt acccctaacc ctaaatatga tttatgtcat gtggtgttag
      361 aagacaccca aggcatgctc tatcaaagtt tggatactga cgttaaacgt tgtgaagata
      421 tccaaaaaac cttggttcaa gccttacaac tcaaagcacg tccaccaaaa cccaaatcta
      481 gtcacagccc acccccattg cctagtcatg tgaaataact ttctcacccc aaccctctcc
      541 caaagggaga cctatttcta aaacgttggg gtgaaatccc tgcccatgca ctaaatgcgc
      601 gggtaaacgc actttgttcg gaatagccca ataacaaagc aatttgatta agctgtaaac
      661 gggggtcaga caaataatgt ttagccaaac gaaaacgggt ttcggctaat aaatgatgaa
      721 aatttaagcc tctgtctgcc aaacgccgat gcaaagtgcg tgttgataaa tttaaggtgc
      781 ttgctacttc atccaaagta ggcacatgtt gatgtaatcg ctcagcacaa aaacggcgta
      841 tcgtttccaa taacaaatca tcatgagtag attggtcaat taatgcctca gcttgtttag
      901 ccaacagttc taacaaataa ggattttggc tattaatcgt taaactcaat gcttcgagcg
      961 gaatatcaat cgacacttgt ttagcattaa aaaatacagg acagccaatc aactcttgat
     1021 ataactgagg atcacaaggc gcaggatgca taaaatgtaa agccacaggt tgcagcccca
     1081 tgccttgcac catctgctta acaaaagtaa ttaaaccact cataaacact tcatcagaca
     1141 attgggttga tgcacctaaa ctcaaatccc aactcaattt tagggcttgc tgatgactgg
     1201 ttaacaacac gtccgacata ttgtgcaata gcttttgaaa acgttgaaaa tgcaacaaag
     1261 cctcacccaa ggttgcacaa tgcataatca aatacgccaa tacacccgta tgttgtggtt
     1321 gcacacattg gccaattttt acccctaaag ctggctcgtg taataacgtt tgcatttcct
     1381 ctaacaattg ccaccatact ttcatgggca tttgggcatg aggcggccat gcggctaatt
     1441 gatgacgaat actgggcgca tcaatctgat atttatctaa ataggcgcgt aataaattgg
     1501 ccaaatcact agcgacataa acaccttgca tacagccctc ttattggcga ttactgttaa
     1561 aaacatggcg aatactgtca atacagtata acaaacaaac ggcaaaatac acctcatcaa
     1621 gtggcactaa agtgccaaat aataaagctt agagggttca agtcatgtct gatgttgttc
     1681 atgccttaat gtttatcgtc ttgtttggtg gattatttat tagtgtcatt gctgctgaag
     1741 catactattg gcacaaaaaa ggccgtcctg aaatgtactc gtttaaagaa acgattacca
     1801 acatgatgat gggcgcaagt tataaaattg ttgatggtat tgctgttgct ttatttattc
     1861 gcgtgttttt tgattgggta tatcaatttg gttttcaata taaacctgtg catggcattt
     1921 ttagccttat tttgattttt ttggtggtcg atgccagctt ttatttatgt cattttatga
     1981 tgcataaagt acgttggttt tggacgtcac acgtcactca tcattcagcc gaacggatga
     2041 attattcaac tgccctacgc caaaatttta ccatggtatt taatggcgca tggttatttt
     2101 ggtgggttcc tgtggcttta gtcggttttg ataaaaactg ggtgacgatt gccattgaat
     2161 taaacttagc ttatcaattc tttttacaca ccgaaacgcg cagcccatta gacaagtttg
     2221 gttttatttt aaatacacca tctcatcatc gtgttcatca tggcagtcaa ccagcacaaa
     2281 ttgatactaa ctttggcggc gtattgatta tttgggataa attgtttggt acttttgttc
     2341 ccgaagaaaa agcaggcaaa attacttatg gcttaactga acgccaacct cattcactta
     2401 atccattttt cttacaagtg catgaatggg ccgatatgtt caaagatttt aaagcgagtg
     2461 gttatgactt tcgagtgttt gtgaaagggc cgggttggaa aagtgataaa atctccactg
     2521 aaaacaataa acacaaacca ttagccaact aaccacaatc aacccgctct atgcgggttt
     2581 ttatttttct ctaggcaata aaaaagcctt gcattgctgc aaggcttttt ttgaattcat
     2641 ggtggcctgg gccg
//
LOCUS       DAOQMB010000323         2649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23194_length_2649_cov_17.023901, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000323 DAOQMB010000000
VERSION     DAOQMB010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23194_length_2649_cov_17.023901"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLY05_09415"
     CDS             complement(<1..224)
                     /locus_tag="PLY05_09415"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNC03557.1"
                     /translation="MLRQRTLKNTIRATGIGLHSGQKVYLALKPHEVDGGIVFRRTDL
                     DPPVDIPSNALLVNETTMSSNLIKDGVKVGT"
     gene            complement(395..1600)
                     /gene="ftsZ"
                     /locus_tag="PLY05_09420"
     CDS             complement(395..1600)
                     /gene="ftsZ"
                     /locus_tag="PLY05_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNC03558.1"
                     /translation="MGIFKIADDQQTHKGLGGPVIKVIGVGGGGGNAVDYMVQSHLQG
                     VEFICINTDLQALNAMTSQKKIQIGVELTRGLGGGGKPEIGRQAALENREDVIDALKG
                     ADMVFITAGMGGGTGTGAAPVVAELARELGVLTVGVVTRPFSFERRDILAEQGIVELS
                     QHVDSLITIPNSKLLKVYGNKITMIEAFKRANNVLFEAVRGISDMITRPGMINLDFAD
                     VRNAMSDRGLAMMGTGVASGADRAREATIKAINSPLLDDIKLEGATGLLVSVAGNADV
                     SLEEFDTVGEIVAPLAEQGVAIKVGMIVDEDIGDDIRVTVVATGLSKAPQLATAGVQA
                     AATVNRPRSPLGTPAPAAPTRNYDEFDTPSVLKKQQTATPAPAAKPEAAPKSDLLNIP
                     SFLRRQNNS"
     gene            complement(1813..>2649)
                     /gene="ftsA"
                     /locus_tag="PLY05_09425"
     CDS             complement(1813..>2649)
                     /gene="ftsA"
                     /locus_tag="PLY05_09425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNC03559.1"
                     /translation="YDIRNPMGMSGVRLEGHYHLVTCGLTALQNLEKCVHNCNLGVDD
                     VVLEPYAAANAVLTDDEKTLGVCLIDIGAGTTDIAVYIHGALRYTEIIPVGGEHVTND
                     IAQVLRTPLAEAEQLKVKQGCAQSISVKREEYINVPTTGDRAQRKLERHVLASIIEAR
                     YLDLFEFIQRRFDELEISDYLHAGIVLTGGAARIEGAAALADSVFGAQKTRIGTPSLR
                     AEGNGQLVQSPVYATSVGLLMAGRDKILADTDKQPVRRTGLSINADGFIAKLRAWLEK
                     NF"
BASE COUNT          795 a          622 c          458 g          774 t
ORIGIN      
        1 gtaccgactt tgacaccatc tttaattaaa ttagatgaca ttgtggtttc attaaccaat
       61 aaagcatttg acggaatatc aacaggcgga tctaaatctg tgcgtctaaa gacaataccc
      121 ccatccacct catgaggttt gagggctaaa taaacttttt gaccgctgtg caggccgata
      181 cctgtagcgc ggatggtatt tttgagggtt cgttgacgca gcattgtgac aaaatgtttc
      241 agagctaaac gctgatacta acatttttta gattttgtgc aagttttgtc tgtaatttaa
      301 ccatactttc ttgcataatt tgcctatatt tacagcaatt aaaacaaaaa ccccctcaat
      361 agagggggtt agtcaattta ttagtttaaa aaatttaact attattttga cgacgtaaga
      421 atgatggaat atttaacaaa tcagactttg gtgcagcttc tggcttagct gctggcgctg
      481 gagtggctgt ctgttgtttt ttcaacacag acggtgtgtc aaattcatca taattgcgtg
      541 ttggcgcagc aggagctggt gtacctaatg gtgaacgtgg acgatttaca gttgctgccg
      601 cttgcacacc tgctgttgct agctgaggtg ctttgcttaa acctgttgca acaacagtta
      661 cacgaatatc atcacctata tcttcatcaa caatcatacc cactttaatc gcaacaccct
      721 gctcagccaa tggtgcaaca atttcaccca cagtatcaaa ctcttctaaa ctaacatcag
      781 cattacctgc aacagatacc aataaaccag tcgcaccttc taatttgatg tcatctaata
      841 atgggctgtt aatagcttta atcgttgctt cacgcgcacg gtctgcacct gatgccacac
      901 ctgttcccat catcgccaaa ccacgatcac tcatggcgtt acgcacgtca gcaaagtcta
      961 ggttaatcat accaggacgg gtaatcatgt cagaaatacc acgcacggct tcaaataaga
     1021 cattgttagc ccttttaaac gcttcaatca tggtaatttt attaccataa acttttaata
     1081 atttagagtt tggaatggta attaacgagt caacgtgttg agataactca acaatacctt
     1141 gttcggccaa aatatcacga cgctcaaaag aaaacgggcg cgtcaccaca cctacggtta
     1201 atacccctaa ctctcgagcc aactcagcaa cgacaggggc agcacctgtg cctgtaccac
     1261 cacccatccc tgcggtaata aagaccatat ctgcgccttt taaagcatca ataacatctt
     1321 cacgattctc taaagcagct tgacgaccta tttcaggctt gcctccacct cccaaaccac
     1381 gcgttaattc tacgccgatc tgtattttct tttgagaagt cattgcattc aatgcttgca
     1441 aatcggtgtt aatacaaata aattctacac cttgtaaatg actttgcacc atatagtcca
     1501 cagcattacc accgccacca ccaacaccaa ttaccttgat aactggacca cctaagcctt
     1561 tatgtgtttg ttggtcatct gcaattttaa aaatgcccat cgaaattctc ctactcaaag
     1621 agtactgttt acaatcatca ctccttgtat gactataagc ctcgtaagat tgacacgtat
     1681 ataagtcacg cgtaaatctt tgttaataaa taaagttgtt gtgttcatta caaacacaat
     1741 agcttttcta ttttaacaat atacaaagag atatgcttag aaaaagtgac tctttgtata
     1801 atatgttaca tattagaaat ttttctctaa ccaagctcgc aacttagcaa taaaaccatc
     1861 ggcattaatg gataagccag ttcgtctgac aggttgctta tcggtatcag ctaaaatttt
     1921 gtctcgaccc gccatcaaca aaccaacact ggttgcataa actggacttt gcactaattg
     1981 accattaccc tcagcacgca aactgggtgt gccaatacgt gttttttgtg caccaaacac
     2041 actgtccgct aaggctgctg cgccctcaat acgtgctgca ccacccgtca agacaatacc
     2101 tgcatgtaaa taatcactta tttctaattc atcaaagcgt ctttgaatga attcaaagag
     2161 atccaaataa cgtgcctcaa taatagaggc taaaacgtgt ctttcaagct tacgttgcgc
     2221 tctatcacct gtagttggca cattaatata ttcttctcgt ttcaccgaaa tagattgagc
     2281 acagccttgt ttgactttta attgttcggc ttccgctaag ggtgtgcgta atacttgggc
     2341 aatatcgttg gtgacatgct caccacccac aggaataatc tcggtataac gtaacgcacc
     2401 atgaatatac acagcaatat cggttgtgcc tgcaccaata tcaatcaaac agacacctaa
     2461 ggttttttcg tcatcggtta atacggcatt agcggcggca tagggttcta aaaccacatc
     2521 atctacacct aaattacagt tatgaacaca tttttctaag ttttgtaagg cagttaaacc
     2581 acaagtgact aaatgataat gcccttctaa acgtacgcct gacatgccca tcggattacg
     2641 aatatcgta
//
LOCUS       DAOQMB010000324         2646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23234_length_2646_cov_10.610189, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000324 DAOQMB010000000
VERSION     DAOQMB010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23234_length_2646_cov_10.610189"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="PLY05_09430"
     CDS             complement(<1..497)
                     /locus_tag="PLY05_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03560.1"
                     /translation="MFKHLLSGSLAALCLLPMQQVFSHEISDEGKIVPILNMGGRYLA
                     TDKDYPSSRLNNALEANGQQAYQQKNALDYIDLGLKVNWADDLSSYVKASYHGVDLGE
                     ELAVEQAWLKYAYDVNHENMLTIKAGRQNVPLGQQNLEHSHNWLMGVTPLVMRASIAG
                     SWLDDG"
     gene            complement(499..1659)
                     /locus_tag="PLY05_09435"
     CDS             complement(499..1659)
                     /locus_tag="PLY05_09435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HNC03561.1"
                     /translation="MAKCVIPVLLSWSLVACNTGTVTEDTSLQSAAWQWQLPANFPVP
                     FVPADNPMTTAKVELGRHLFYDLRLSGNGTQACGTCHLQEKAFTDGLAVAKGSTGEFH
                     PRNSQHLANVVYNTTLTWANFSLSSLERQMEVPMFGEDPIELGINDKNKEEVLQRFVQ
                     DSQYQQLFSQAFPEQNQAINYANIIKAIASFQRALISGNSRYDQSLTGKATLTASEIR
                     GKDLFFGEKAECFHCHGSFNFNDQVRHAGSRIVETPFHNTGLFNIGGTGEFPFPNRGI
                     FELSGKAEDMGKFRASSLRNIAVTAPYMHDGSIATLEAVLDFYAAGGRNITEGIHAGD
                     GRKNPFKSELVTRIDLNEQEKADIVAFLKTLTDDEFLTNPKFANPFTQAKQP"
     gene            complement(1861..>2646)
                     /locus_tag="PLY05_09440"
     CDS             complement(1861..>2646)
                     /locus_tag="PLY05_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="HNC03562.1"
                     /translation="NKTVEIRDLRFYVAEVKLINDKNEAVAVALKESPENFNQRYGVT
                     LLDFEDATGVCAEVGTADTYTTIQGTIPQGNYTGIQFILGVPDTGVDSTGKTIALSHT
                     ETTSMLAPLDVAAMAWSWQGGRKFVKVEVNPVGGVLNQKGTADTSDDANEKVWTVHLG
                     ATGCTDKGATESPLTRYECSNPNLTTIKLPTFDYTKQKVVLDIPALFMGSDVALNKVA
                     ALGCMSGKPDTDCNAVFDALGINLETGKTSTTKAQSIFKVVNK"
BASE COUNT          803 a          572 c          459 g          812 t
ORIGIN      
        1 ccatcatcta gccaactccc tgcaatactg gctctcatca ctaaaggtgt aacacccatt
       61 aaccaattat gactatgctc taaattttgt tgacccaaag ggacattttg ccgccctgct
      121 ttgatagtta acatattctc gtgattaaca tcataagcat atttaagcca tgcttgctct
      181 acagctaact cctcacccaa atctacaccg tgataagaag ctttaacata actacttagg
      241 tcatctgccc aattaacttt tagccctaaa tcaatgtaat ctaaagcgtt tttttgttga
      301 taagcttgct gaccattcgc ctctaaagca ttatttaaac gactgcttgg ataatcttta
      361 tcagttgcta aatatcgtcc acccatattt aaaatcggga ctattttgcc ttcatcgcta
      421 atttcgtgac taaacacttg ctgcatgggt aacaaacata aggcggctaa actaccgctt
      481 aatagatgct taaacatatt agggctgctt cgcttgcgta aatggattgg caaatttagg
      541 attagttaaa aactcatcat ccgtgagtgt ttttaaaaaa gcgacaatat cggctttctc
      601 ttgctcattg aggtctattc ttgttactaa ttcactttta aaaggatttt tgcgaccatc
      661 ccctgcatga attccctcag taatattacg tccacctgcg gcataaaaat ccaaaacagc
      721 ctctaaggtt gctatagaac catcgtgcat ataaggtgcg gtaaccgcaa tattacgcaa
      781 actagaggca cgaaacttgc ccatatcttc agctttgcca gataattcaa aaatgccacg
      841 attggggaaa ggaaattcac ctgtaccgcc aatattaaat aagcctgtat tatgaaatgg
      901 tgtttctaca atgcgtgaac ctgcatgacg cacttggtcg ttaaaattaa aactaccatg
      961 acaatgaaaa cattcggctt tttcaccaaa aaataaatct ttaccgcgta tttcgctagc
     1021 agtcagcgtg gctttgcctg ttaatgattg gtcataacgc gaatttcccg aaatcaaggc
     1081 acgttgaaaa ctggcaatcg ctttaataat attagcgtaa ttaatcgctt gattttgttc
     1141 tggaaatgct tgactaaata attgttgata ttggctatct tggacaaaac gctgtaacac
     1201 ttcttcttta tttttatcgt taatccctaa ctcaataggg tcttcaccaa acattggcac
     1261 ttccatttgg cgttctaagc tgcttagact aaagtttgcc catgtgagcg tggtgttata
     1321 cacaacattg gctaaatgtt gagagttacg cggatgaaac tctcctgttg agcctttagc
     1381 aacggccaaa ccatcggtaa atgctttttc ttgcaaatga caagtaccac aagcttgtgt
     1441 gccattaccc gataaacgca gatcataaaa tagatgccgt cccaattcga cttttgcagt
     1501 cgtcattggg ttatcggcag gcacaaatgg cacaggaaaa ttggcaggta attgccattg
     1561 ccatgccgct gactgtaagg acgtatcttc tgtgactgta cctgtattac aagccactaa
     1621 agaccatgat aaaagcacag gaattacgca ttttgccaaa attagcgcgt tttgcgagca
     1681 taaaatcgtt aaaaacgatt gaaatgcgaa caaataagcg ataaccgcgt aagtcctaaa
     1741 caaagttaaa agaggatatt tcatagttta taaacaaaaa aactcatgta gctaaggttt
     1801 tgccacatga gttataaatc aaaataaaag tttagcttga gattttagca cagccagtta
     1861 ctacttatta accactttaa agatagattg tgctttggtg gtactggttt tgcctgtctc
     1921 taaattaata cctaaagcat caaaaaccgc attacaatca gtatcaggtt tacccgacat
     1981 acaacccaat gccgcaactt tgtttaaagc cacatctgaa cccatgaata atgcgggaat
     2041 atccaacact actttttgtt tggtataatc aaacgttggt agtttaatag tggttaaatt
     2101 tggattagaa cactcataac gtgttaaggg tgattcggtc gcgcctttat cggtacaacc
     2161 tgttgcgcct aaatgcactg tccaaacttt ttcattagca tcatcagagg tatctgcggt
     2221 acctttttga ttaagcacac caccaacagg attaacttcg actttgacaa atttacgacc
     2281 accctgccaa ctccatgcca tagcagccac atctaaaggt gctaacattg atgttgtttc
     2341 ggtatgactt aaagcgatgg ttttacctgt actgtcaacg cccgtatcag gtacgcctaa
     2401 aataaactga atccctgtat aatttccttg tggaattgtt ccttgaattg tcgtataagt
     2461 gtctgctgtt cctacttcgg cacatacgcc tgttgcatct tcaaaatcta ataatgtcac
     2521 gccataacgt tgattaaaat tttcaggact ttcttttaac gccaccgcta ctgcttcatt
     2581 tttgtcatta atcaatttca cttcggcaac ataaaaacgt aaatcgcgaa tttcaacggt
     2641 tttatt
//
LOCUS       DAOQMB010000325         2636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23401_length_2636_cov_16.601705, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000325 DAOQMB010000000
VERSION     DAOQMB010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23401_length_2636_cov_16.601705"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..497)
                     /locus_tag="PLY05_09445"
     tRNA            complement(421..497)
                     /locus_tag="PLY05_09445"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(461..463),aa:Arg,seq:ccg)
     gene            complement(592..680)
                     /locus_tag="PLY05_09450"
     tRNA            complement(592..680)
                     /locus_tag="PLY05_09450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Leu,seq:taa)
     gene            complement(710..783)
                     /locus_tag="PLY05_09455"
     tRNA            complement(710..783)
                     /locus_tag="PLY05_09455"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(749..751),aa:Cys,seq:gca)
     gene            1079..1930
                     /gene="folD"
                     /locus_tag="PLY05_09460"
     CDS             1079..1930
                     /gene="folD"
                     /locus_tag="PLY05_09460"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HNC03563.1"
                     /translation="MSALVLDGKKLASLIEVELSARVAVIKEKTQQIPILATILVGDD
                     PSSATYVRMKGNACRRVGMDSLSVILPASTTTEQLLAQIQELNANPNVHGILLQHPVP
                     HQIDERACFDAIDLKKDVDGVTCLGFGRMSMGEEAYGSATPQGIMTLLKHYQVELAGK
                     HAVVVGRSAILGKPMAMMLLAANATVTICHSKTQNLAELVKQADIVVGAVGKPRFIKA
                     EWIKDGAVVVDAGYHTVDGGVGDIELAPLTERVSAYTPVPGGVGPMTIATLMTQTVMA
                     AEKAMAT"
     gene            2027..2302
                     /locus_tag="PLY05_09465"
     CDS             2027..2302
                     /locus_tag="PLY05_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HNC03564.1"
                     /translation="MSITVQNLEAWELPRHHTFKVLGEMQYPLAQVVADIAIKHCPSF
                     DAAKISMRSSSGGKYLSVTVEVYLETKDQINGLYEDFNAAPEIKLVY"
     gene            2359..>2636
                     /gene="lipB"
                     /locus_tag="PLY05_09470"
     CDS             2359..>2636
                     /gene="lipB"
                     /locus_tag="PLY05_09470"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013357092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HNC03565.1"
                     /translation="MRTFTDQRTDQTADELWLLQHPAVFTLGQAGKEHHILMAGDIPV
                     VKTDRGGQVTYHGQGQLVGYCLFDVKRLGIGVRELVEKIEDILVAVLQ"
BASE COUNT          767 a          444 c          601 g          824 t
ORIGIN      
        1 ctgcaccagt acttgcacct gcggctgcac ccaacatgcc accagctttt ttgccataat
       61 gacgtttagt aatttgtctt gattgacaat ttggacaatg tttagccatg atgaataatc
      121 ctaagaaaca ataattcata aaaagataag atgaaggtaa gacttcatta agatgatata
      181 tatttgaaaa attttggaat gaggtcatgg ttaaaaattg caatgttata tcattacaat
      241 ttttgagcat tgttatttgg ctaaaaactc agtagaagag agtgatgaaa tgaccgtaaa
      301 aagaaaaaag gtgtagttgt aaatagagga gtgtcaggat ctaatagcta tttaatttcc
      361 gtacagattc cgtatgagaa aattttatcg attttatttg ttgtaactta ttgatttaaa
      421 tggcgcgccc ggcgggattc gaacccacga cccctgcctt cggagggcag tactctatcc
      481 agctgagcta cgggcgcgta agagttgcaa tataacgttt tactattatt tgagcaataa
      541 aaaacccttg caatgcaagg gttcttgtat atacagcgca tgctgttata atggtgccta
      601 gggccggaat cgaaccggca cggctgtcac cagccgaggg attttaagtc ccttgtgtct
      661 accaatttca ccacctaggc aaaaatcttt tcagcgtcac taacctaact ggaggctgag
      721 gtcggaatcg aaccggcgta cacggagttg cagtccgctg catgaccact ctgccaccca
      781 gcctttaatt tgctagctgc ctgctgagga cgcctatgtt atatagaaag aaaaaaaacg
      841 tcaacacttt ttttcttttt gataatcaag tgattataaa atagactttt tgtctaaaag
      901 ttgatctttt tgtttgattg cttggcttta aacaaaagaa agttgtctgt catgcataaa
      961 taatcttcgt gaaaaaacaa ctaactttgg tgtgggtaat gctaaaatag ccacagcttg
     1021 cgtttagacg ttggttcaac cgttccttaa tcttaaaagt tttaataaca ggttttttat
     1081 gtctgcttta gttcttgatg gtaaaaagct cgcttcttta atagaagttg aattatctgc
     1141 acgagtggct gtaattaaag aaaaaactca acaaattccc attttagcaa ccattctggt
     1201 gggtgatgat ccatcttccg caacctatgt gcgtatgaag ggcaatgctt gtcgtcgtgt
     1261 gggtatggat tctttatcag ttatcttgcc tgctagcacc acaactgaac aattattagc
     1321 acaaattcaa gagttaaatg ccaatcctaa tgtgcatggt attttgttgc agcatcctgt
     1381 ccctcatcaa attgatgaac gtgcttgttt tgatgcaatt gatttaaaga aagatgttga
     1441 tggtgtcaca tgtttaggct ttggtcgtat gagcatgggt gaagaagctt atggttctgc
     1501 aacaccacaa ggcattatga ctttattaaa gcattatcaa gtagagttgg cgggcaaaca
     1561 tgcggttgtg gtcgggcgta gtgccatttt gggcaaacca atggcgatga tgttgttggc
     1621 agctaatgcc accgtgacga tttgccattc taaaacccaa aacttagctg aattagtgaa
     1681 gcaagccgat attgttgttg gtgcggtagg taaaccacgt tttattaaag cagagtggat
     1741 caaagacggc gctgttgtgg ttgatgctgg ttatcatacg gttgatggtg gtgtgggtga
     1801 cattgagtta gcacctttga cagaaagagt gtctgcttat acgcctgttc ctggtggtgt
     1861 tggcccaatg accattgcaa cattaatgac tcaaacggtc atggccgccg aaaaagcaat
     1921 ggcaacttga ttttagaaga tttggccgta tttataaggt tgaaaaagac atgcaccatg
     1981 tttgttaata tggtgtatgt tttattgttg ttgaagagag tgaattatga gcataactgt
     2041 gcaaaatctt gaggcatggg agttgccacg tcatcatact tttaaagtat taggagagat
     2101 gcaatatcct ttagcacaag tggtggcaga tattgcgatt aaacattgtc caagttttga
     2161 tgcagctaaa attagcatgc gttcttctag tggtggtaaa tatttatcgg tgactgtgga
     2221 agtttattta gaaaccaaag atcaaatcaa tggtttgtat gaagatttta atgcagctcc
     2281 cgaaattaaa ttggtttatt aaaattgtta ttgattaagg atttaggctg cttgccgttt
     2341 gaacaggttt ggcagcaaat gcgtacattt actgatcaac gcaccgatca aacggctgat
     2401 gagttatggt tgttgcaaca tcctgctgtg tttacgcttg gacaagcagg taaagagcat
     2461 catatcttaa tggcaggtga tatacctgtg gttaaaaccg atcgtggtgg ccaagtcact
     2521 tatcatggtc aagggcagtt ggtaggatat tgtctgtttg atgtgaaacg attaggtatt
     2581 ggtgttcgag agttagtaga aaaaattgaa gatattttag tagccgtttt gcaaga
//
LOCUS       DAOQMB010000326         2626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23552_length_2626_cov_17.321276, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000326 DAOQMB010000000
VERSION     DAOQMB010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23552_length_2626_cov_17.321276"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..2609
                     /locus_tag="PLY05_09475"
     CDS             114..2609
                     /locus_tag="PLY05_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC03566.1"
                     /translation="MNINRPHLLRSTTGLVVIALCVVASITVILAFQPYIQHEKQLIE
                     QHQHQKAELIADDLDNTFNTISQQILNLSILSASYSNNPTLLEQTLTNLLRASSPENI
                     YGMGIWFKQGQSPHPRYPLYGPYAYYQNKKMTLTYDWMSESYNFPKQEWFKIILAGQG
                     QQQCTKPYLDNGVMYVSCGRSFPMSPTAKGVVTIDIILPQLDELLAKYSTNQQEIILI
                     SDSKNQHLIAHPHSQALLKQFKQAKSILDIKISDILPTKDQKQWIILEKKLNMGWSIQ
                     VLSRKTWIEREVNALNQRLYMWLLFIWLAGVLIDSFWMYSTKRIRNALNSSVVWRNAL
                     SDVIPTGIFSANFDGQITWANPVFSKLTYQDKFPTPLIDIICFEDQPKFRLFWHRFCT
                     EGKVSATEFKLRMGTQKWVSLRLALASEHNLDTPTIAGVLDDVSERRRHEQELRHAKD
                     QAEEANRSKGEFLAMMSHEIRTPMNGVIGMSSLLLDTPLNTEQRDFAETIQNSANILL
                     KIINDILDVSRIEAGKLPIENYAFRTESLITEVINLITPITEQKHLPLYKDISEHLPL
                     MLEGDADRIKQVLLNLLNNAIKFTETGSITLKIEINHLDQQNAHLTFNVIDTGIGLSE
                     EQQRRIFQPFTQADSSTTRRYGGTGLGLTICRHLVELMGGEIHCESQLGQGSRFWFRL
                     PLKISQQQTINPEKTQISLPTFAQQFTILVVEDNPVNQQVVCKMLQKLGLHYEIAQNG
                     REALMQLSSSNLFDLVLMDCQMPVMDGFEATKRWRNQEQVLTLRRIPIVALTANAMQG
                     DEERCLEAGMDAHLAKPINLEALATVLAKWLTV"
BASE COUNT          839 a          514 c          473 g          800 t
ORIGIN      
        1 cccctacaat aaaaatcttg cttttgattc tgtgcagcca agatgttctg tgttaagttc
       61 tgaatgaggc tttttaatca gccatactcg cttttattat tattagtaaa cacatgaata
      121 tcaaccgccc acacttactt cgttcaacga caggtcttgt cgtcatcgct ttatgtgttg
      181 tcgcgagtat tactgttatc ttagcctttc aaccctatat tcaacatgaa aaacaactga
      241 tcgaacaaca tcaacatcaa aaagctgaat tgattgctga tgatcttgat aatacattta
      301 atacgattag ccaacagatt ctaaatttaa gtatattaag tgctagttat tctaataatc
      361 ccactctctt agaacaaact cttaccaatc tactgcgtgc ctcttcgcca gaaaatattt
      421 atggtatggg aatttggttt aaacaagggc aatcgccaca tccacgctat cccctttatg
      481 ggccttatgc ctattatcaa aacaagaaaa tgactctaac ttatgactgg atgtcagaaa
      541 gttataattt tcccaaacag gaatggttta aaatcatttt agctggccaa ggtcaacaac
      601 aatgtacaaa accttatttg gataatggtg tgatgtatgt cagttgtggg cgtagttttc
      661 cgatgagtcc cacagcaaaa ggagttgtca ccattgatat aatattgcct cagcttgacg
      721 agttattggc caaatacagc acaaatcaac aagaaatcat tcttattagt gacagtaaaa
      781 accaacactt aattgcacat cctcatagcc aagctttatt aaaacaattc aaacaagcta
      841 aaagcatttt ggatattaaa attagcgata ttttaccaac taaagatcaa aaacaatgga
      901 ttatcttaga aaagaaactc aatatgggtt ggtctattca agttttaagc cgaaaaacgt
      961 ggattgaacg tgaagtaaat gcacttaacc aacgtttata tatgtggtta ttatttattt
     1021 ggttggcagg cgttttaatt gatagttttt ggatgtatag caccaaacgg attcgtaatg
     1081 ctttaaatag ctctgttgtt tggcgtaacg ccttaagcga tgtgattcca acaggtattt
     1141 tttcggccaa ttttgatggt caaattacat gggctaaccc cgttttttct aaattaacct
     1201 atcaagataa attccctacc cccttgattg atattatttg ttttgaagat caacccaaat
     1261 ttagactttt ttggcatcgc ttttgtaccg aaggcaaagt ttcggccact gagtttaaac
     1321 tacgaatggg tacacaaaaa tgggtatcct tacgtttggc tctcgcctct gaacataatt
     1381 tagatacgcc aactattgca ggcgtattag atgatgtgag tgaacgtcgc cgtcacgaac
     1441 aagaattgcg tcatgctaaa gatcaagctg aagaagccaa tcgcagtaaa ggtgaatttt
     1501 tggccatgat gagccatgaa attcgcactc ccatgaatgg cgtcattggc atgtcgagct
     1561 tattacttga tactccactc aataccgaac aacgtgactt tgctgagacc attcaaaact
     1621 ctgctaatat tttattaaaa attattaatg atattttaga tgtttcaagg attgaagcag
     1681 gcaaacttcc tattgaaaac tatgctttcc gtacggagtc actcattaca gaagtcatta
     1741 atttaattac ccccattacc gaacaaaaac atttacccct ttataaagat atatccgaac
     1801 acttaccgct catgctggaa ggagatgctg accgtatcaa acaggtatta ctaaatttgt
     1861 taaataatgc cattaaattt accgaaacag gctccatcac gcttaaaatt gaaattaatc
     1921 atttagacca acaaaatgcc cacttaacct ttaatgtcat tgatacaggt attggcttaa
     1981 gcgaggagca acaacgacgt atttttcaac cctttactca agccgacagc tcaacgacac
     2041 gccgttatgg tggcacaggt ttaggcttaa ctatctgccg tcacttggtt gaattgatgg
     2101 gtggcgaaat tcattgtgaa agccaattag gtcagggttc acgtttttgg tttagattgc
     2161 cactcaaaat tagtcagcaa caaactatta accctgaaaa aactcaaatt agtttaccta
     2221 cttttgcaca gcaatttacc attctggtgg ttgaagacaa cccagtcaat caacaagttg
     2281 tgtgtaaaat gctacaaaaa ttgggcttgc actatgaaat agcacaaaat ggtcgtgaag
     2341 cactgatgca actgagtagc tctaatttgt ttgatttagt gctgatggac tgtcaaatgc
     2401 ctgtcatgga tggttttgaa gcaaccaaac gttggcgtaa ccaagaacaa gtattaactt
     2461 tgcgtagaat tcctattgtc gcactgacag caaatgccat gcaaggcgat gaagagcgat
     2521 gtttagaagc aggaatggat gcgcatttag ccaaaccaat taacttagaa gctttagcga
     2581 ctgtgttggc taaatggtta actgtgtaaa actatccctt cgactc
//
LOCUS       DAOQMB010000327         2619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23666_length_2619_cov_5.888456, whole genome shotgun sequence.
ACCESSION   DAOQMB010000327 DAOQMB010000000
VERSION     DAOQMB010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23666_length_2619_cov_5.888456"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..2075
                     /gene="brxL"
                     /locus_tag="PLY05_09480"
     CDS             3..2075
                     /gene="brxL"
                     /locus_tag="PLY05_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system Lon protease-like protein BrxL"
                     /protein_id="HNC03567.1"
                     /translation="MNEHFSGLVVRKDLVKTVKGNAIVPSYVLEYLLGQYCASNDELT
                     IQNGISTVKEILRKHYVHRNESGLVRSIIKEKGRHKVIDRISVALNEKKDAYEAEFAN
                     LGIKKVIIDSHTVKAHPKLLVSGVWCIADVEYDFSEDNDASPWILGSLKPIQLSHLDF
                     DAYTQARRFFSTNEWIDMLIQSMGFEPSQFSKRNKFNQLVRLIPFCERNYNLIELGPK
                     GTGKSHIYSEFSPHGILISGGEVTTPKLFVHNGTGKVGLVGYWDTIAFDEFAGKKKRV
                     DKALVDIMKNYMANKTFSRGIETLGAEASMVFVGNTQHSVPHMLKHSDLFDELPESYH
                     DSAFLDRIHFYIAGWEVDIIRGEMFSQGYGFVVDYLAEVLRALRSHDYSDRYKPYFTL
                     SSDLSTRDRDAINKTFSGLMKILYPDGQACESDIQELLNFAMEGRKRVKDQLLRIDNT
                     YSQVSFSYRNQRDEIHHITTKEEDTYPNYYHKKTNISDTAISASYQDDALFAIPSTSI
                     TNESATKTNDQVKEKSELTEQQITRPEGATGFSLHDCITPYLQQATEITISDPNIGLI
                     HQQDNLLELLTTIANAKSQTQQSNVQLIITTDLFIEEQQDSDLQHIAKACLDVGINLT
                     WQFVPSTDITESFIQTNTGWRIRVHQGLDIYQPYSFNDKFNLMKRLPDYRVCKAFEMD
                     IVSISAEQ"
     gene            2178..2549
                     /locus_tag="PLY05_09485"
     CDS             2178..2549
                     /locus_tag="PLY05_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03568.1"
                     /translation="MMNLYTIEAELNYHDLPVNLKEGWSSAFGAAFLDYVGENQIIKY
                     PVSINGRLTKKCIFIGDEQLANEFVAYLQKNADKKKRSIKFMVRKIDYTDLNYRRKTA
                     QAEANTNDAKIRETLELFCYN"
BASE COUNT          857 a          483 c          509 g          770 t
ORIGIN      
        1 aaattaatga gcatttttcg ggtttagtgg tgcgtaaaga cctcgtaaaa acagtcaaag
       61 gtaatgcgat tgtcccttct tatgtgttgg agtatttgtt gggtcaatat tgtgccagta
      121 atgatgaatt aactattcaa aatggcatta gtaccgtcaa agagatttta cgtaaacatt
      181 atgtacatcg taatgagtca ggcttggtgc gctcgattat taaagaaaaa ggtcggcaca
      241 aagtcattga tcgtatcagt gtcgcactca acgagaaaaa agatgcctat gaagccgagt
      301 ttgctaactt aggtattaaa aaagtcatta ttgattctca taccgtaaaa gcgcatccca
      361 agttattggt cagtggcgtt tggtgtattg cggatgttga gtatgacttt agtgaagaca
      421 atgatgcttc gccgtggatt ttaggctcat taaaacccat ccagctctca catctagatt
      481 ttgatgctta tactcaagcc agacgctttt tttctaccaa tgaatggata gatatgctca
      541 tacaaagtat gggctttgaa ccttctcagt tcagtaaacg taataaattt aaccaactcg
      601 tgcgcctgat tcctttttgt gagcgtaact acaacctaat tgagttaggc cctaaaggaa
      661 caggtaaatc gcacatttac tccgaatttt cgccacatgg aattttaatt tcaggcggag
      721 aagtcaccac gcctaaattg tttgttcata atggcacagg taaagtaggt ttagttggtt
      781 actgggacac tatcgctttt gatgagtttg caggaaaaaa gaagcgtgtt gacaaagcct
      841 tagtcgatat tatgaaaaac tacatggcca acaaaacttt ttctcgtggt attgaaaccc
      901 ttggcgcaga agcatcaatg gtatttgtgg gtaacaccca gcacagtgtt cctcacatgc
      961 ttaagcacag cgacttattt gacgaactcc ccgaaagcta tcatgactcc gcgtttcttg
     1021 accgcattca tttttatatt gctggttggg aagttgatat tattcgtggt gaaatgtttt
     1081 cgcagggcta tggctttgtc gttgattatt tagccgaagt attacgcgct ttacgcagtc
     1141 acgattattc cgaccgctac aagccctatt ttacgctatc gtctgattta tcaactcgtg
     1201 atcgtgatgc cattaacaaa acattttcag gcttaatgaa aattttatat cctgatggtc
     1261 aagcgtgtga aagtgatatt caagaactgc ttaattttgc gatggagggg cgtaaacgag
     1321 tcaaagacca actgctccgc atagataaca cctatagcca agtctctttt agttatcgca
     1381 atcaacgtga tgaaatacac cacatcacca ccaaggaaga agacacttac ccaaactact
     1441 accacaaaaa aaccaacata agcgacacgg ctattagtgc gtcatatcaa gacgatgcgt
     1501 tatttgctat acctagcaca tcaatcacca acgaatcagc aacaaaaacg aatgaccaag
     1561 ttaaagaaaa atcagaatta accgaacaac agataacacg gccagaaggc gcaacaggct
     1621 ttagtctgca tgattgtatt acgccttatt tgcaacaggc gactgagatt accatcagtg
     1681 acccgaacat cggtttaatc caccaacaag acaacttact agaactatta actacgattg
     1741 ccaatgccaa atcacagacg caacaaagta acgtccagct cattatcact accgatttgt
     1801 ttatagaaga acaacaagac agtgatttac aacatatcgc taaggcatgc ttagatgttg
     1861 gcattaattt aacgtggcaa tttgtaccat caacagatat cactgagtct tttatacaaa
     1921 caaatacagg ttggcgaatt cgggtacatc aaggtttaga tatttatcaa ccctattcat
     1981 ttaatgataa atttaatctg atgaagcgac ttcctgacta tcgtgtttgt aaagcttttg
     2041 aaatggatat tgtgtcgatt agtgcagaac aatagtgtta ttagggatgt tatcaatcaa
     2101 ttggtttgga ctattgttaa ttaagctaaa aattatacaa ttgaaaaagt tagtattttt
     2161 aaatataagg agattgaatg atgaatctat acaccattga agccgaacta aactaccatg
     2221 atttgccagt taaccttaaa gaaggttggt cgtcagcttt tggagcagct tttttagatt
     2281 atgtgggtga aaatcaaata attaagtacc cagtaagcat caacggacga ctaacgaaaa
     2341 agtgtatttt tattggtgat gaacaacttg ctaatgaatt tgtggcctat ctgcaaaaaa
     2401 acgctgacaa aaagaaacgc tcaatcaaat ttatggtgcg taaaattgat tatactgatt
     2461 tgaattatcg aagaaaaact gctcaagcag aagcaaacac aaatgatgca aaaattagag
     2521 agactttgga gttgttttgt tataactaaa aaaaagccct tcaaatgaag taatgccagt
     2581 cagttaagag ctgactggca tttttttata ggatatttt
//
LOCUS       DAOQMB010000328         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_23988_length_2597_cov_14.880409, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000328 DAOQMB010000000
VERSION     DAOQMB010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23988_length_2597_cov_14.880409"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2105)
                     /gene="nirB"
                     /locus_tag="PLY05_09490"
     CDS             complement(<1..2105)
                     /gene="nirB"
                     /locus_tag="PLY05_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HNC03569.1"
                     /translation="MRIVMIGHGMVGHRFLENLAKKATVPHEVIVLAEEPRPAYDRVQ
                     LSAFFSGKTAEDLSVTPNDFFSNTGYGLQLNTRATGIDTAAKTVTTQNGDVFHYDKLV
                     LATGSYPFVPPVQGNNREGCLVYRTIEDLYAIKETAASSKVGVVVGGGLLGLEAAKAL
                     KDLGLETHVVEFAPRLMAVQIDDMGGKVLRKKIAELGVGIHTERNTVEIVDGANCRHA
                     MKFSDGTSLETDMILFSAGIRPRDDIARANGITVGPRGGIVVDNFCRTNAPDVYAIGE
                     CALWDGKIYGLVAPGYQMAEVACTHILGNDDKQFTGADMSTKLKLMGVDVASIGDAHG
                     ATAGSLNYSYIDERNQVYKKLVVSNDGKKLLGAVLVGDVEAYGDLLQLKLNDMTLPEK
                     PEALILPATDGSKPAGLGVDALPMTAVICSCNNVTKQDLCDAIEGGAMELGELKACTK
                     AATSCGGCSALVGQVLKCELQKRGVEVKKDICEHFAYSRQEMYHLAKIGNIRTFEDFI
                     AKHGKGDGCDICKPTAASIFASLWNEHILKKPLAGLQDTNDYFLANMQKDGTYSIVPR
                     IPGGEITPEGLIIIGEIAKKYKLYTKITGGQRIDLFGARVEQLPVIWKELVDAGFESG
                     HAYGKSLRTVKSCVGSTWCRYGVQDSVGFAIDLENRYRGLRSPHKLKFAVSGCTRECA
                     EAQSKDVGVIATEKGWNLYV"
     gene            complement(2244..>2597)
                     /locus_tag="PLY05_09495"
     CDS             complement(2244..>2597)
                     /locus_tag="PLY05_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HNC03570.1"
                     /translation="QRYAWLDGDNLTALAFFGAEADLPPRAWLMSLLNQPLDKLSRRA
                     LLSGKPADPNADVGRIICACFGVGEKTIERAIATNNLKSVAEIGKCLKAGTNCGSCQP
                     ELQKILSRLIPVAQA"
BASE COUNT          744 a          610 c          499 g          744 t
ORIGIN      
        1 acatataagt tccagccttt ttcggtggca atcactccta cgtctttgct ttgtgcttcg
       61 gcacattcac gcgtacaacc actcaccgca aatttgagtt tgtgtggact acgtaaaccg
      121 cgataacgat tttctaaatc aatggcaaaa ccgaccgagt cttgtacccc ataacgacac
      181 catgttgaac caacacaaga ttttacggta cgtaacgatt taccgtaggc atgacctgat
      241 tcaaaacctg catcgactaa ctctttccaa atgacaggca attgttcaac ccgcgcacca
      301 aataagtcga tacgttggcc acctgtgatt ttggtgtaca gtttgtattt tttggcaatt
      361 tcaccaatga tgattaaacc ttcaggtgta atttcgccac caggaatacg tggcacgatg
      421 gaataagtac catctttttg catattagct aagaagtagt cattggtgtc ttgtaagcct
      481 gctaagggct ttttgagaat atgttcattc cacaacgagg caaaaattga ggcggcagtg
      541 ggtttacaaa tatcacaacc atcgcctttg ccatgtttag caataaaatc ttcaaacgta
      601 cggatgttgc caattttggc aaggtgatac atttcttgac gtgaataagc gaagtgttcg
      661 caaatgtctt ttttgacttc cacaccacgt ttttgtaatt cacattttag tacttgtccg
      721 actaaggcag aacaaccacc acaactggtt gctgctttgg tgcaggcttt taactcgcct
      781 aactccattg caccgccttc aatggcgtca cacaaatctt gtttggttac attgttacaa
      841 gaacaaatca ccgctgtcat gggtaaagca tcaacaccca aaccagcagg tttgctacca
      901 tctgtggctg gtaaaattaa ggcttctggt ttttctggca aggtcatatc gttaagcttg
      961 agctgtaata aatcgccata agcttcaaca tcacctacta acaccgcacc taataacttt
     1021 ttaccatcat tagaaacaac aagcttttta taaacttgat tacgttcatc aatatagctg
     1081 tagtttaaac tgcccgccgt tgcaccatgt gcatcaccaa ttgacgcaac atccacaccc
     1141 attaatttga gtttggtgct catgtctgcg ccagtaaatt gtttgtcgtc attgcctaaa
     1201 atatgggtac aggccacttc tgccatttga tagcctggtg ccaccaagcc ataaattttg
     1261 ccatcccaca aagcacactc accgattgca tagacatcag gggcattggt acgacagaag
     1321 ttgtcaacaa caataccgcc acgtggccca acagtaatac cattcgcacg agcgatatca
     1381 tcacgtggac gaatacccgc agagaataaa atcatgtcag tttcgaggct agtaccatca
     1441 gagaacttca tggcatgacg acagtttgcg ccatcaacaa tttcaacagt gttacgctcg
     1501 gtgtgaatgc ctacgcctaa ttcggcaatt tttttgcgta atactttgcc acccatatcg
     1561 tcaatttgca ccgccattaa acgtggggca aattcaacaa cgtgagtttc taaacctaag
     1621 tctttcaaag ctttagcagc ttctaaacct aacaagccac caccaacaac cacacctact
     1681 tttgagctag cagctgtttc tttaatggca tataaatctt cgatggtacg atataccaag
     1741 caaccttcac ggttgttacc ttgtacaggg ggtacaaaag gataagaacc tgtggctaat
     1801 actaatttgt cataatgaaa cacatcacca ttttgtgtag tgaccgtttt ggcagcagta
     1861 tcaatgcctg tggcacgagt atttaattgc aagccataac ctgtgttact aaagaaatcg
     1921 tttggtgtaa cactgaggtc ttcggcggtt ttaccactaa agaaagcgga taattgtaca
     1981 cggtcatacg caggacgtgg ttcttctgcc aacacaataa cttcgtgtgg cactgttgct
     2041 tttttggcaa ggttttctaa gaaacggtga ccaaccatac cgtgaccaat catgactata
     2101 cgcattgctg actcctgctg tatttgctag ctagaggtac ctgtttaaac ggggtactaa
     2161 cagacggcgt tgtctgcggg tatttaacag aacacgtttg ttgatactgc caacattacc
     2221 agtatccgtg tttgttgctc agtttaagcc tgagcaacgg gaattaagcg agaaaggatt
     2281 ttctgtaact ctggctgaca tgatccacaa ttcgtgccag cttttagaca tttaccaatt
     2341 tctgccacac tttttaaatt atttgtagca attgctcgtt ctatagtctt ttctcctacg
     2401 ccaaaacagg cacaaataat tcttccaaca tcggcattag ggtcagcagg cttacctgac
     2461 aataaagcgc gacgactgag tttatctaaa ggttgattta acaaagacat taaccatgcg
     2521 cgtggcggta aatctgcttc agcaccaaag aaagccaatg ctgttaaatt atcaccatcc
     2581 aaccatgcat aacgttg
//
LOCUS       DAOQMB010000329         2576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24334_length_2576_cov_13.138040, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000329 DAOQMB010000000
VERSION     DAOQMB010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24334_length_2576_cov_13.138040"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="PLY05_09500"
     CDS             complement(<1..1100)
                     /locus_tag="PLY05_09500"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004723762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC03571.1"
                     /translation="MCAASAGQRGRRVLVLDSSNKVGKKILMSGGGRCNFTNLFVESS
                     NYLSANPHFCISALNRYTQWDFIALVEKHRIAYHERKHGQLFCNDSAKDILAMLLAEC
                     ERTQHVTIQTHCQIEKISPVDDGEHRFHLQTSKGQFACQSLVIATGGLSIPTMGASAF
                     GYEVAKQFGHQVLPTRAGLVPFTFTDNIKAVTERLSGLALDVTMTAKDNTSFNENLLF
                     THRGLSGPVALQLSNYWQLGETVAVNLLPQLEMNNWLKEQKQQHPKSLLRTQLIPLLS
                     KNLVLELQALHWPSYAETPLAEIPDSVLLTVAEALQAWPLKPSGTEGYRTAEVTLGGV
                     DTRELSSKTMESKKQKGLYFIGEVVDVTGWLGG"
     gene            1255..2250
                     /locus_tag="PLY05_09505"
     CDS             1255..2250
                     /locus_tag="PLY05_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03572.1"
                     /translation="MYSDEDLDSAINANILNQESVKNFRQFIAQQRHSALNDEEHFRL
                     ISGFNDVFVSVACLLFLFSLSWLGSQLNSMLSALLVMIGSWLLAKPFVIKRRLALPAI
                     VLLFSYVGSFFWLSLILFTLIYEDAAALGAGIATLVGAYVHWRYFKVPITIAAGVACL
                     VGSVISFISEFSTLKTFMPAVFFMGGLFCFMYAMWWDSQDRHRQTRKSDVAFWLHLLA
                     APLIVHPIFTSIGIFDTNSNLLQISLVLLLYASLAIVSLIVDRRALMLSALFYALYAL
                     THMLKTYGSLDQGFALAGLMIGGALLILSVFWHSTRALLIRHLPIAYQHKLPTVQ"
     gene            2266..2499
                     /locus_tag="PLY05_09510"
     CDS             2266..2499
                     /locus_tag="PLY05_09510"
                     /inference="COORDINATES: protein motif:HMM:NF022435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HNC03573.1"
                     /translation="MDTSKHTLATLFAQLGLANSPEDINQFIATHQISAGTPLAQASF
                     WTPAQANLIRKALADDADWSEVVDSLAVRLSAS"
BASE COUNT          709 a          553 c          497 g          817 t
ORIGIN      
        1 ccacctaacc agcctgtcac atccactact tctccaataa aatacaagcc tttttgtttt
       61 ttactttcca tcgtcttaga cgatagctct cgtgtatcca caccacctaa agttacttct
      121 gcggtacgat atccttctgt gcctgatggc tttaatggcc aagcttgcaa agcctcagcc
      181 acagttaaca acacactatc aggtatttca gccaatggcg tttcggcata acttggccaa
      241 tgcaaagctt gtaattccaa cactaagttt ttacttaata atggaattaa ctgtgtacgt
      301 aataatgatt taggatgttg ctgcttttgc tcttttagcc aattattcat ttctaattgt
      361 ggcaacaaat tcactgctac cgtttcgcct aattgccaat agttggataa ttgcaaagca
      421 acaggcccgc ttaagccccg atgggtaaat aacaaatttt cgttaaaact ggtgttgtct
      481 ttggcggtca tggtcacatc taaagctaaa cccgatagac gttctgtgac tgccttaatg
      541 ttatccgtaa aggtaaatgg cactaaacct gcgcgtgttg gcaacacctg atgaccaaac
      601 tgtttagcca cttcataacc aaaagcactc gcccccatcg tgggaatcga caaaccgcct
      661 gtggcaatca ctaacgactg acaagcaaat tgacctttac tggtctgcaa atgaaaacga
      721 tgctcgccat catcgactgg gctaattttt tcaatttgac aatgggtttg aatggtcacg
      781 tgttgagtac gttcgcactc tgccaacaac atggctaaaa tatctttagc cgagtcatta
      841 caaaataatt gaccgtgttt acgttcgtga taagcaatgc gatgcttttc aaccagtgca
      901 ataaaatccc attgagtgta acgattgagt gcagaaatac aaaaatgtgg gtttgcagat
      961 aaataattac tagactcaac aaataaattg gtaaaattac aacggccacc gcctgacatt
     1021 aaaatctttt taccgacttt attgctacta tctaacacca acacgcgacg gccacgctgt
     1081 cctgcactgg ctgcacacat taatcccgag gcaccgccac ctataataat gacatcaacg
     1141 tagttcaaag aggtattctc tatttcaaaa acaaactagg cgttccattt tatcaaaaaa
     1201 cacttcatta attatgctat tttgtgggta taacctaaaa ataggacatt ccctatgtac
     1261 agtgatgaag accttgattc cgctatcaat gcaaacatac tcaatcaaga gtctgtaaaa
     1321 aactttagac agtttatcgc acagcaacga catagtgcct taaatgatga agaacatttt
     1381 aggttaattt cgggctttaa cgatgtattt gtcagcgttg cctgtttact ttttttattc
     1441 tctttatcat ggttaggctc acagcttaac tccatgttga gtgctttatt agtgatgata
     1501 ggttcttggt tattggctaa gccttttgtt atcaagcgtc gtctagcttt gccagccatc
     1561 gtgttactat ttagttatgt gggtagtttt ttttggctct ctcttatttt atttacctta
     1621 atttatgaag atgctgctgc attaggtgca ggcattgcaa ccttagtcgg ggcatatgtt
     1681 cattggcgat actttaaagt tccgattacc attgcggcag gtgttgcgtg tttagtgggc
     1741 agtgtcatca gttttatatc cgaattttcc acgttaaaaa cgtttatgcc tgctgtcttc
     1801 tttatgggtg gtttgttttg ttttatgtat gcgatgtggt gggatagcca agaccgccat
     1861 cgtcaaacac gcaaatctga tgtcgctttt tggttgcatt tattagctgc gccgctgatt
     1921 gttcacccca tttttacgtc aataggtatt tttgatacca atagcaattt gctgcaaatt
     1981 agcctagtgt tattgttgta tgcaagtttg gcaattgttt ctttaattgt ggatagacgt
     2041 gcgctcatgc tttcggcctt gttttatgcc ctatatgcac tgacacacat gttaaaaacc
     2101 tatggctcat tagaccaagg ctttgcttta gcaggattaa tgattggcgg tgcattactg
     2161 atattgtcag tattttggca tagcactcgc gcgttattaa ttcgtcattt gcctattgcg
     2221 tatcaacaca aactacctac ggtacaataa atcacggaga taatcatgga taccagcaaa
     2281 cacacgcttg cgactctttt tgcacaactc ggcttagcca atagtccaga agacattaat
     2341 caatttattg ccacgcatca aatcagtgcg ggtacgccct tggcacaagc atctttttgg
     2401 acacctgcac aagcaaatct tattagaaag gcattagcag acgatgcaga ctggagcgaa
     2461 gtggttgata gtttagcggt acgtcttagc gcaagttaat gtgtgccatt gattgcacta
     2521 aaaaactatt atcgctacaa tctcacgtta ctgccgaggg gtgctgcacc agttaa
//
LOCUS       DAOQMB010000330         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24358_length_2575_cov_5.814286, whole genome shotgun sequence.
ACCESSION   DAOQMB010000330 DAOQMB010000000
VERSION     DAOQMB010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24358_length_2575_cov_5.814286"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1964
                     /locus_tag="PLY05_09515"
     CDS             <1..1964
                     /locus_tag="PLY05_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03574.1"
                     /translation="IINQQLSEWQQYFEQQSQALQQQQQIINQKLAQLLSNNGDLHQQ
                     KAQLQQQLQQLNNTSQRQALLAQQFALVANRQQLETQLAAVSNQLDQQTALVQQASSR
                     PLASIERELTRCQQEIVNTERELASLSDNLYLALQQQLESQQLDLINRLFNKQVMLLD
                     SQHYQLNSTLFTQWLSKNAIENLELAGLTLKLEQISAQHSQSSAEQLQQRLQELKQTL
                     ADYQQQVAAAKAIEQAKQRKLALAQEVKHIERDLADFDELTGLNNSSEQRQQQQQQCQ
                     QQLASIDSQLTQFQAYYQQQQTHASQLSEKLTQLNDKHQEINRQRNQRKDDAVVFAYL
                     DELPHQPWLTISEPVLDDLANNLAAYHKDCQTLINLDEQMKTRLAELHAGGLTKFQYS
                     EQPEQEIRRIIEFAELLPQEAQSLERKARDAVINVTVCLRQLRDGLLTFKSKMKEFNN
                     LINRRQISDLSKFKIEPQEETALVEAINLLINTAEKVNTGDTFELFNQASVLDDEALN
                     RAKNLLVGEGKAHDFLRVEHLFRLSFIVAKMNGAPESFEDIDSAASNGTVLMAKLVTG
                     LALLHLMQDKRHQVRAVCYLDEASALDIRNQKILIDTAEEFGFSLIFASPTPLITARY
                     CVPISRHQGWNQISQKSWQILSNKEDSVL"
     gene            1961..>2575
                     /locus_tag="PLY05_09520"
     CDS             1961..>2575
                     /locus_tag="PLY05_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03575.1"
                     /translation="MSRPLIDALNKLIVANSMLPSSQFSPKQQQALNSFSQQTNAISQ
                     RPQGRGTVYQVINLPRVELELKTLQPINAENLANDLPQRSINIGLYRNSKAKQHNHDV
                     SYILMKAISPSVIWQKENGHYLDVSENTRLSGVASLMITASDNWCSQQPLWLVENQAL
                     FDNLSWLPDDATGTIVYYAGQLHGVLLNWLAQKPRCREVILFPDY"
BASE COUNT          887 a          498 c          503 g          687 t
ORIGIN      
        1 caatcattaa ccaacaactc agcgaatggc aacagtattt tgagcagcaa agccaagcat
       61 tacagcaaca gcagcagatt attaaccaga agttagcaca actactcagt aataacggcg
      121 atttacatca gcaaaaagca caattacaac agcagttaca gcagcttaat aacactagcc
      181 aacgtcaagc cttattagca caacaatttg ctttggtcgc taaccgtcaa caattagaaa
      241 cacagcttgc cgctgtaagc aatcaattag accaacaaac ggcattagta cagcaagcca
      301 gtagtcgtcc tttggcgagt attgagcgtg aattaacacg ttgtcagcaa gaaattgtta
      361 acactgagcg agaactagcc tcattgagcg ataatttgta tttggcctta caacagcaac
      421 tcgaatcaca acaacttgat cttataaata ggctgtttaa caaacaagtg atgttattag
      481 atagtcagca ttatcaatta aatagcacgt tatttacgca atggttgagt aaaaacgcca
      541 tagaaaacct cgagctagca ggtttaacct taaagctaga gcaaattagc gcacaacaca
      601 gtcagtcaag tgccgaacaa ctccaacagc gattacaaga gcttaaacaa acattagcag
      661 attatcagca acaagttgca gctgccaaag ccatcgagca agctaaacaa cgtaaattag
      721 ccttagcaca agaagtcaaa catattgagc gtgatttggc agattttgat gaactaacag
      781 gcttaaataa cagcagcgag caacgccagc aacaacagca gcagtgccaa caacaattag
      841 ccagtattga cagtcaactg acacagtttc aagcatacta ccaacaacag caaacacacg
      901 ctagtcagct gagtgagaaa ctcacccaac ttaacgataa acatcaagag attaaccgtc
      961 aacgtaatca acgcaaagat gatgcagtcg tatttgctta tttagatgaa ttgccccatc
     1021 aaccgtggct gactatcagc gaacctgtgt tagatgactt agcgaataat ttagctgcct
     1081 atcacaaaga ctgccaaacg ctgattaacc tcgatgagca aatgaaaacg cgtttagctg
     1141 agttacacgc aggaggctta actaagtttc agtacagcga acagccagaa caagaaatta
     1201 gaagaattat tgaatttgca gaacttttac cccaagaagc tcaatcttta gagcgtaagg
     1261 cgcgtgatgc agtcattaat gtgacagtgt gtttgcgtca attgcgagat ggtttgttga
     1321 cttttaaaag caaaatgaaa gagtttaata atttaatcaa tcgtcgtcag atatctgatt
     1381 tatctaaatt caaaattgag ccgcaagaag aaacagcatt agttgaggca attaatttat
     1441 taattaacac agcggaaaag gtgaacacag gtgatacctt tgaactattc aatcaagcta
     1501 gtgtcctaga tgatgaggct ctcaatagag ctaaaaattt gttagtaggc gaaggtaagg
     1561 cgcatgattt tttaagggta gaacatttat ttagactctc atttattgta gcaaaaatga
     1621 atggtgctcc cgaaagtttt gaggatatag atagtgcagc ttctaatggt acggtattga
     1681 tggctaagtt ggtaacaggt ttagctctat tacatttaat gcaggataaa cgtcatcaag
     1741 tacgtgcggt ctgttattta gatgaagcct ctgctttaga tatacgcaat caaaaaatcc
     1801 ttattgatac agctgaagaa tttggattct cattaatttt tgcctcacca accccactca
     1861 ttactgcgcg ttattgtgtc cctattagcc gtcatcaagg atggaatcag attagtcaaa
     1921 agagttggca aattttatct aataaagaag atagtgtgct atgagtcgtc ctttgattga
     1981 tgcactaaat aaactcattg tggcaaacag catgttgcca agtagtcaat ttagccccaa
     2041 acaacaacag gcattaaata gcttttccca acaaacaaac gcgattagtc agcgtccaca
     2101 aggcagaggt actgtctatc aagtgatcaa tttgccgcgt gttgagttag agcttaaaac
     2161 cttacaaccg ataaatgctg aaaacttagc taatgacttg cctcaacgta gtattaatat
     2221 tggcttatat cgaaacagca aagctaaaca acataaccat gatgttagtt atatattgat
     2281 gaaagccatt agtccatcgg tgatttggca gaaagaaaat ggtcactatt tggatgttag
     2341 tgagaatact cggttatcag gtgttgctag tttaatgata acagcatcag ataattggtg
     2401 ttctcaacaa cctttgtggt tagttgaaaa tcaagcctta tttgataact tatcatggtt
     2461 gccagatgat gcgacaggaa cgattgttta ttacgcagga caattacatg gtgtgttatt
     2521 aaattggttg gcgcaaaaac cacgttgtcg tgaggttatt ctatttcccg actat
//
LOCUS       DAOQMB010000331         2574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24372_length_2574_cov_16.537912, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000331 DAOQMB010000000
VERSION     DAOQMB010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24372_length_2574_cov_16.537912"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="PLY05_09525"
     CDS             complement(<1..143)
                     /locus_tag="PLY05_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC03576.1"
                     /translation="MRIIFAGTPVFAAESLRAILHTQHEVVGVYTQPDRPVGRGRELK
                     PSPV"
     gene            complement(262..768)
                     /gene="def"
                     /locus_tag="PLY05_09530"
     CDS             complement(262..768)
                     /gene="def"
                     /locus_tag="PLY05_09530"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNC03577.1"
                     /translation="MAKLPILHFPDSRLRTKAKPVSVVDDAIRQLVKDMFETMYEAPG
                     IGLAATQVNHHIRLVVIDITEDKSQPLVFINPEITPLTEDKAPYEEGCLSVPTVYDVV
                     ERPTRIKVNALNEQGEAFEMEVDGLLAVCLQHELDHLEGKLFVDYLSTLKRQRIKTKL
                     QKLQKQQA"
     gene            1170..2270
                     /locus_tag="PLY05_09535"
     CDS             1170..2270
                     /locus_tag="PLY05_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HNC03578.1"
                     /translation="MKKLVLLALVSAMFSAPLQADVKSSDVKESAPERYTVKKGDTLW
                     DIANRYLQDPWRWPEIWQANQQVRNPHLIYPGDKLLLCHIEQRAVVAVDDGGGCEGIL
                     ANMTNKASVSGGSGNVKLSPEIKVENLELAIPAIHLKDIRAYLNDGRVLDASEFEKAP
                     YVLSASDKRVLAGQGDIVYIRGGKDLVLGESLSVYRKGVRYDDPDTKEYLGFEAEDIA
                     SGRIIAINGEVATLQLTRGTQEVRIGDRLFVNEARAITPIFYPSNPSDVKTGRILRIM
                     GSLGSGGLNSVIVLNRGERDGVKQGNTFAIYQRGGVITDRLKGDLVRLPSERAGLAMV
                     FRTFDKVSYAIILRASTRIAIGDEVRPPISGD"
     gene            2321..>2574
                     /locus_tag="PLY05_09540"
     CDS             2321..>2574
                     /locus_tag="PLY05_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03579.1"
                     /translation="MSQFLFVDWLIVWRLVEQSTSSFYKLYRQFTSPKLALLSTAQHW
                     QQAGVNEKLALNLAAWQQHDAKLYADIQHSLDFIQQQQISV"
BASE COUNT          791 a          467 c          548 g          768 t
ORIGIN      
        1 acaggactgg gtttgagttc tcggccacga cccacagggc ggtctggttg ggtataaacg
       61 ccaaccactt catgttgagt atgtaagatt gcacgtaaag attctgccgc aaagacaggt
      121 gtaccagcaa agatgatacg caggggactg gccaagaaaa taaacctcag aatgtaggat
      181 gggttgagga atgaaaccca tcaataaatc caattataca ataacaaaaa attaaaggct
      241 tggtgttaat gccaacacaa atcaagcctg ttgtttttgc agtttttgca atttcgtttt
      301 aatacgttga cgttttaagg tagataaata atcaacaaat aacttacctt ctaagtggtc
      361 aagctcatgt tgcaaacaaa cagctaataa accatccact tccatttcaa aggcttcgcc
      421 ttgctcattc aacgcattca ctttaatgcg agttggacgc tcaaccacat cataaacggt
      481 tggtacagat aaacaacctt cttcataggg ggctttatct tcagtgagtg gtgtaatttc
      541 gggattaata aaaaccaaag gttggctttt gtcttcggta atatcaatga ctaccaaacg
      601 aatatgatga ttcacttgtg tagccgccaa accaataccc ggtgcctcgt acatggtttc
      661 aaacatatct ttgactaatt gacgaatagc atcatccacc acagagacag gtttagcttt
      721 tgtgcgcaag cgagaatcag gaaaatgcaa aataggaagt tttgccataa tacgaataac
      781 ctatagatat tagacggggc taggccgaaa atgtgatttc ttcaaagaaa acagacaata
      841 agcctttgag agttcacaaa acacaataaa atccctaatc aagggcaatg gtgagcattg
      901 ccaagctgag taagtgcctc tacaatcacg agcataacgc tattataagc gattcgttta
      961 aatggcacat aaacaaataa agcaaaaatt taattaatac ggtttaatct gtgttttaag
     1021 tgcttagact aaatctagga cttacgcggt tatcgcttgt ttgttcatat tttaaccgtc
     1081 tttgacggtt ttatgctcac aaaacgcgct aattttggca aaatgcgtaa gccctataat
     1141 caacagtccc gccaacaagg gaatagataa tgaaaaaatt ggtgttactc gcgctcgttt
     1201 cggcaatgtt tagcgcacca ttgcaagctg atgttaaatc ctctgatgtg aaagaaagcg
     1261 cacctgagcg ttacacggtg aaaaaaggtg atactctttg ggatattgcc aatcgttatt
     1321 tgcaagaccc ttggcgttgg ccagagattt ggcaagccaa ccaacaagtt cgtaacccac
     1381 acttaattta tccaggtgac aaattattat tatgtcatat tgaacagcgt gctgttgttg
     1441 cagtagatga tgggggtggt tgtgagggca ttttagccaa tatgactaac aaagcctctg
     1501 ttagtggtgg tagtggtaat gttaagttaa gccctgaaat taaagttgaa aacttagagt
     1561 tagccattcc agctattcac ttaaaagata ttcgcgccta cttaaatgat ggtcgtgttt
     1621 tagatgcctc tgaatttgaa aaagcccctt atgtattatc tgcatcagat aaacgcgttt
     1681 tagcaggaca aggtgatatt gtttatattc gtggtggtaa agatttagta ttaggtgaga
     1741 gtttgagtgt ttatcgtaaa ggtgtacgtt acgatgatcc agacaccaaa gaatatttag
     1801 gctttgaagc agaagatatt gcttctggcc gtattatcgc aattaatggc gaggttgcga
     1861 ctttacagtt aacacgtggc actcaagaag ttcggattgg tgatcgttta tttgttaatg
     1921 aagctcgtgc aattacgcct attttttatc caagcaatcc aagtgatgta aaaacagggc
     1981 gtattttgcg tattatgggg tctttagggt cgggcggctt aaatagtgta atcgtactaa
     2041 atcgcggtga acgagatggc gttaaacaag gcaatacctt tgccatttat caacgtggtg
     2101 gtgtgattac tgatcgttta aaaggcgatt tagtgcgttt gccctcagag cgtgcaggtt
     2161 tagctatggt attccgtact tttgataaag taagttatgc cattatctta cgtgcatcga
     2221 cccgtattgc tataggtgat gaagtacgtc caccaatcag tggtgattga ttattaaaag
     2281 gctgttttca cagcctttta ttttggcaaa aggatttgtg atgtcccaat ttttatttgt
     2341 agattggtta attgtgtggc gtttagttga acaatcaacc agtagttttt ataaattata
     2401 ccgtcaattt acatctccta aactggcttt gttgtcaacg gcacaacatt ggcaacaagc
     2461 aggcgttaac gaaaaattag ctcttaattt ggctgcatgg caacaacatg atgctaaatt
     2521 atatgcagat attcaacaca gcttagactt tattcagcaa caacaaatca gtgt
//
LOCUS       DAOQMB010000332         2569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24461_length_2569_cov_14.651551, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000332 DAOQMB010000000
VERSION     DAOQMB010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24461_length_2569_cov_14.651551"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..>1208
                     /locus_tag="PLY05_09545"
     CDS             4..>1208
                     /locus_tag="PLY05_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNC03580.1"
                     /translation="MSFTKVLIANRGEIACRVIRTAKQLGYHTVAVYSDADCNAPHTQ
                     LADEAIHIGESNVSASYLSIDKIIAAAKTTGADAIHPGYGFLSENEDFATACANNQIT
                     FIGPPANAIYAMGSKRQAKIMMLKAGVPCVPGYEGDNQDEQFLAAQAEKIGLPVMIKA
                     SAGGGGRGMRCVTQASELLASIRTARSEAQNAFGSGELILEKAVINPRHVEIQVFADQ
                     HGNCVYLGERDCSIQRRHQKVVEEAPCPVMTPELRQAMGEAAVAAAKAVNYVGAGTVE
                     FLLDNQGEFYFLEMNTRLQVEHPVTELITGQDLVAWQLRVAAGEPLPLRQEQIQLNGH
                     AIEVRLYAEDPQFNYLPQTGKILRWQAATGAQVRIDDGVQTGGEVSPFYDPMIAKVIA
                     WGATRQEAAR"
     assembly_gap    1209..1308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1309..1824
                     /locus_tag="PLY05_09550"
     CDS             <1309..1824
                     /locus_tag="PLY05_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013196811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methylcrotonyl-CoA carboxylase"
                     /protein_id="HNC03581.1"
                     /translation="TLPAKFLLLTIGEQKQHVQVTAPLHSQQPWLLQVNEQTFAIDIY
                     QNNNGLLRYAINGHRRQLNYALANNSVYLDAGQGQLVARDDTHSPPQTADAASQNKIL
                     APMDGLLVNILAKVGDTVSAGQTLLLLEAMKIEHPLKAPCAGVVKEILVHEKQQVKKR
                     QLLLVIEPNAV"
     gene            1842..>2569
                     /locus_tag="PLY05_09555"
     CDS             1842..>2569
                     /locus_tag="PLY05_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC03582.1"
                     /translation="MSLKNQTVFITGASRGIGRAIALRCAREGANVVIAAKSNEPHPK
                     LGGSIHSVADEVVAAGGQALAILLDVRDEVAVQKAMQQAAEHFGGIDILINNAGAIAL
                     TNVENTPLKKYDLIQSINHRAVFVCSQAALPYLKKSSNGHILSLSPPINLNPKWLTVF
                     APYTLSKYGMTLLSIGMAQEFKDYGICVNTLWPETYIATAAVTNNIANDEAVDICRKP
                     DIMADAAFAIISHRETGQHYLDEGV"
BASE COUNT          703 a          552 c          550 g          664 t
ORIGIN      
        1 attatgtctt ttaccaaagt attaattgct aaccgtggcg aaattgcttg ccgcgtgatt
       61 agaaccgcca aacaactcgg ctatcacact gttgccgttt actcggatgc cgattgcaat
      121 gcccctcaca cccaattagc cgatgaggca attcatattg gcgaatcgaa cgtctctgca
      181 tcttatttat ctatagacaa aattattgct gccgccaaaa caacaggggc agatgcgatt
      241 caccctggtt atggcttttt gtctgaaaat gaagactttg ctaccgcttg cgccaataac
      301 caaattacct ttattggccc acctgctaat gctatttatg cgatgggcag caaacgtcaa
      361 gccaaaatca tgatgctcaa agcaggcgtg ccttgtgtac caggttatga aggcgacaat
      421 caagatgagc aatttttagc cgcacaagcc gaaaaaattg gcttacccgt gatgattaaa
      481 gcctccgcag ggggtggtgg tcgtgggatg cgctgtgtta cccaagccag cgaattattg
      541 gcctcgattc gtactgctcg ctctgaggca caaaatgcct ttggtagtgg tgagttaatt
      601 ttagaaaaag cggttatcaa cccacgccat gttgaaatac aagtatttgc cgaccaacat
      661 ggcaactgtg tgtatttggg tgaacgcgac tgttcgattc aacgccgcca ccaaaaagtg
      721 gttgaagaag ccccttgtcc tgtcatgaca ccagaattac gtcaagcgat gggcgaagct
      781 gctgttgctg ctgccaaagc ggtgaattat gtgggtgcag gtacggttga gtttttatta
      841 gataatcaag gcgagtttta tttcttagaa atgaataccc gcttacaggt tgaacatcct
      901 gtcaccgaat tgattacagg ccaagattta gtcgcttggc aattacgtgt tgccgctggt
      961 gaacctttac cgcttcgcca agagcaaatt caactcaatg gccatgccat tgaagttcgt
     1021 ttgtatgctg aagacccaca atttaactac ttgccacaaa caggcaaaat cttacgctgg
     1081 caagcggcaa caggcgcaca agtaaggatt gatgatggtg tgcaaacagg tggtgaagtc
     1141 agtccctttt atgacccaat gattgccaaa gtcattgcat ggggagcaac acgccaagaa
     1201 gcagcacgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnac tttgcctgct
     1321 aaatttttgt tattaaccat tggtgaacaa aaacaacatg tgcaagtcac tgccccactc
     1381 catagccaac agccttggtt attgcaagtg aatgagcaaa cttttgcgat tgatatttat
     1441 caaaataaca acggcttatt acgttacgct atcaacggtc atcgtcgcca actcaactat
     1501 gccttagcca ataacagcgt ttatttagat gcagggcaag gtcaacttgt cgctcgtgat
     1561 gacacccaca gcccaccaca aaccgctgat gcggcaagtc aaaataaaat cctagcacca
     1621 atggatggtt tgttagtcaa tatcttggca aaagttggcg acaccgtgag tgcaggtcaa
     1681 actctgttgt tactcgaagc gatgaaaatt gaacatccac tcaaagcgcc gtgtgctggg
     1741 gtggtcaaag aaattttagt ccacgaaaaa caacaggtaa aaaaacgtca attgttgttg
     1801 gtgattgaac cgaatgcagt ttaattaatt gggagaatcc tatgtcttta aaaaatcaaa
     1861 ccgtctttat tacgggtgct agtcgtggta ttggtcgcgc cattgcctta cgttgtgcgc
     1921 gtgaaggggc taatgtggtg attgctgcca aatcaaacga gccacatcca aaactgggtg
     1981 gtagcattca tagtgtggct gatgaagtgg ttgctgctgg tggccaagcc ttagctattt
     2041 tgctagatgt tcgtgatgaa gtcgcggtac aaaaagccat gcaacaagcc gcagaacatt
     2101 ttggtggcat tgatatttta attaacaatg ctggtgccat cgccctgacc aatgttgaaa
     2161 acaccccctt aaaaaaatat gatttaattc aatccatcaa tcatcgtgcg gtatttgttt
     2221 gtagccaagc ggctttgccc tatctaaaaa aatccagcaa tggccatatt ttgagtttat
     2281 caccacccat taacctcaat ccaaaatggt taacggtgtt tgcaccttat accttatcca
     2341 aatatggcat gacgttattg tcaattggca tggctcaaga atttaaagac tatggcattt
     2401 gtgtcaacac tttgtggcca gaaacctata ttgccacagc cgccgtcact aacaatattg
     2461 ccaatgatga agccgtcgat atttgccgta aacctgacat tatggctgat gccgcctttg
     2521 ccattattag ccaccgcgaa acaggccaac attatttaga tgaaggggt
//
LOCUS       DAOQMB010000333         2567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24498_length_2567_cov_14.086783, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000333 DAOQMB010000000
VERSION     DAOQMB010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24498_length_2567_cov_14.086783"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2072)
                     /locus_tag="PLY05_09560"
     CDS             complement(<1..2072)
                     /locus_tag="PLY05_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03583.1"
                     /translation="MRFDRFTAKLQQALGEAQSLAVGKDHTTIDAVHLLLAFIQQQGG
                     SIKPLLTQSGVNMASLDKKLNELLERAATVNKPTGDVQIGQELQRVFNLADKLAQQRQ
                     DQFIASELVLLAMIEQGGDTAKALQQAGLNKQQLQQAIQQLRGDSPVTDSNAEDNRQA
                     LSKYTIDLTERAAKGKLDPVIGRDDEIRRTIQVLARRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLRNKKVLSLDMGALIAGAKFRGEFEERLKAVLNDLAKQEGNIILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYVEKDAALERRFQ
                     KVLVDEPSVTDTIAILRGLKERYELHHGVDITDPAIIAAAKMSHRYITDRKLPDKAID
                     LIDEAASRIRMEMDSKPEVMDKLDRRIIQLKMEREALKQEEDKASKVQLELVEGQIQK
                     LEREYADLNEIWQAEKASVKGSQNIKEELEKAKFAFEQAQRKGDTNEMARLKYGVIPD
                     LEKQLHQADDAEQSTHQLLRNKVTEVEIAEVVSKATGIPVAKMMEGDREKLLRMEEAL
                     HQRVVGQHEAVEAVSNAVRRARAGLSDPNRPSGSFLFLGPTGVGKTELCKALANFLFD
                     TDDAMIRIDMSEFMEKHSVSRLIGAPPGYVGYEEGGYLTEAVRRKPYSVVLLDEVEKA
                     HPDVFNIL"
BASE COUNT          678 a          598 c          464 g          827 t
ORIGIN      
        1 aataaaatat taaacacatc gggatgggct ttttccactt catccaacaa caccacggaa
       61 tatggcttgc gacgcacagc ttcggttaaa taaccgccct cttcataacc gacataacct
      121 ggtggcgcac caattaagcg tgacacactg tgtttttcca taaactcaga catatcaatc
      181 cgaatcatcg catcatcggt atcaaacaag aaattcgcta atgccttaca taactcggtt
      241 ttaccgaccc ctgttggccc taaaaataag aaagaaccac tagggcgatt ggggtcagac
      301 aagcccgcac gcgcacgacg taccgcatta gatactgctt ctactgcttc atgttggcca
      361 acgacgcgct gatgtaaagc ttcttccata cgcaataatt tttcgcggtc accttccatc
      421 attttagcca caggaatgcc tgttgctttg gaaacgactt cggcaatttc tacctctgtg
      481 actttatttc tcaatagttg atgtgtgctt tgctcggcat catcggcttg atgaagttgc
      541 ttttccaaat caggaatcac cccatattta aggcgtgcca tttcattggt gtcgccttta
      601 cgttgagctt gctcaaaggc aaacttggct ttttccaatt cttctttaat gttttgtgag
      661 cctttaacac tggctttttc tgcctgccaa atttcgttaa ggtcagcata ttcacgctct
      721 agcttttgaa tttggccttc aactaactct aactgcactt ttgaggcttt gtcttcctct
      781 tgttttaagg cttcacgctc catttttaat tgaataatac ggcggtcaag tttatccatg
      841 acttcgggtt tggagtccat ttccatccga atacgtgagg ctgcttcatc aattaaatca
      901 atggctttat ctggtaactt acgatcagtg atataacgat gtgacatttt tgctgcggca
      961 ataatcgcag ggtcagtaat atccacacca tgatgcaact cataacgctc ttttaggcca
     1021 cgtaaaatgg caatggtatc ggtcacactc ggctcatcaa ccaatacttt ttggaaacga
     1081 cgttctaaag cggcatcttt ttcgacatat tgacgatatt catctaaagt tgttgccccg
     1141 acacaatgca gctccccacg cgctaaagca ggcttgagca tattgcctgc atccatcgcg
     1201 ccatcggctt tacctgcacc caccatcgtg tgcaactcat caataaataa aataatattg
     1261 ccttcttgtt tggccaagtc attcagcacc gcttttagac gttcttcaaa ttcacctcta
     1321 aattttgcac ctgcaatcaa tgcgcccatg tctaaagaca acactttttt attgcgtaaa
     1381 ccttctggca cttcaccatt aataatacgt tgtgctaaac cttcaacaat cgcggtttta
     1441 cccacaccgg gttcaccaat taacacagga ttgtttttgg tacgccgtgc caacacttga
     1501 atggtgcggc gaatttcgtc atcacgacca atgacagggt ctaatttgcc tttggcagcg
     1561 cgttcggtta aatcgatggt gtatttactt aaggcttgac gattgtcttc ggcattacta
     1621 tcagtcacag gactatctcc gcgtaattgt tgaatcgctt gttggagttg ttgtttgttt
     1681 aagcctgctt gttgtaaggc tttggcggtg tcgccaccct gctcaatcat cgccaacagc
     1741 actaactcac tggcaataaa ttggtcttgg cgttgttgag ctaatttatc agccaaatta
     1801 aacacacgct gtaactcttg gccaatttgt acatctcctg ttggcttatt aaccgtcgcc
     1861 gcacgttcta ataattcatt cagcttttta tctaagctcg ccatattcac gcctgattgc
     1921 gttaataaag gtttaatcga gccaccttgt tgctgaataa aggctaataa taaatgtacc
     1981 gcatcaatcg tagtatggtc tttacctacc gctaaggatt gagcctcacc taatgcttgt
     2041 tgtaatttgg cagtaaaacg atcaaatctc ataatttagt tcctacttgt ctttgactta
     2101 gcacgctcta agtcttaaaa aaattaaggt tattgtagaa atgaggtata gacaaaagat
     2161 ttcaagacgc aaatttttct tttttcataa accaacttga gcacttgaaa acaaacaaag
     2221 gctgcccatt tagatgcatt tttaacaggc tttctccact aaatcttact tatccgcata
     2281 gtcacttaat gaaaatctct gattgtttca ttttatgcaa tagtgtattt ctttttgtgt
     2341 acttgatagt acatattctt tcattaaaat acccaataga tcttgactat tttactcggt
     2401 tataaatgtt tatgaggtgt attatgcggc ttattttggc tgcggcctca ttatctttag
     2461 tgcttgtttc aacatctgcc catagtgaca acaataataa caatcagcta ggactagagc
     2521 ctaatgatag tggttggtcg cttaatctcg acaacgattt gtttacc
//
LOCUS       DAOQMB010000334         2562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24597_length_2562_cov_15.854009, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000334 DAOQMB010000000
VERSION     DAOQMB010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24597_length_2562_cov_15.854009"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLY05_09565"
     CDS             complement(<1..227)
                     /locus_tag="PLY05_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03584.1"
                     /translation="MTHHYPDLTLPYERHYLGLYQGQVKDSFFELPQANEAAKTWLWI
                     DEIVNNNDELLQGIHSPHEYSGYDLHVRKQPV"
     gene            complement(362..2530)
                     /locus_tag="PLY05_09570"
     CDS             complement(362..2530)
                     /locus_tag="PLY05_09570"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004701954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HNC03585.1"
                     /translation="MTERVQRGELAIAKELYDFIVNQAIEGTGISVDDYWTKFEAIVA
                     DLMPKNKALLAKRDAIQAQIDAWHQANPKFDFAAYKAFLTEIGYLVAEGEDFSVNPQN
                     VDDEIARIAGPQLVVPVKNARYALNAANARWGSLYDALYGNDVISEEGGADKGKGYNP
                     ARGAKVIAYARTLLDTAAPLATGSHADSVAYAIVNGALEVSLKDGSKVGLASPEKLVG
                     YAGDLSAPTSILLKNNGLHIDIQFDGNHPVGQQDPAKIKDLVIESALTTICDCEDSVA
                     AVDAEDKVEVYGNWLGLMKGNLVESFEKGGKTLTRTMNADRTYTALNGGSLTLHGRSL
                     LLVRNVGHLMTNPAVLYKGEEIPEGIMDALVTVLIAKHDLLGNGSLKNSRTGSVYIVK
                     PKMHGPEEVAFANEIFTRVEEALGLAQNTLKMGIMDEERRTTVNLKECIRAAKDRVMF
                     INTGFLDRTGDEIHTSMEAGAVIRKADMKKAKWIASYENWNVDIGLACGLQGKAQIGK
                     GMWAMPDLMKDMVEQKIAHPMAGANTAWVPSPTGATLHSMHYHKVNVFARQDELKSRA
                     RANVDDILTIPLAENTDWSAEEIKNELDNNCQGILGYVVRWIDQGVGCSKVPDINNIG
                     LMEDRATLRISSQHVANWLRHGICTQEQVLEAMQRMASVVDGQNANDPLYKPMAPNFD
                     DSVAFQASKELVFEGCAQPNGYTEPVLHRRRRELKAKIAG"
BASE COUNT          739 a          592 c          468 g          763 t
ORIGIN      
        1 acaggctgtt tacgcacatg taaatcataa ccgctgtatt catgtgggct atgaatccct
       61 tgcaataact catcattatt attaacaatt tcatcaatcc atagccatgt tttggcggct
      121 tcatttgcct gtggcaattc aaaaaaactg tcttttacct gaccttggta taatcccaaa
      181 taatgtcttt cgtaaggcaa ggttaaatca ggataatgat gcgtcataaa aactcctttt
      241 agtcgtattt acccccctgc cttcggcatc cccccttaat aaggggggac tgaggggggt
      301 agcaataaaa aaggacactt acagctttcg ccataagtgc ccttctttta gcgtctaaaa
      361 attaaccagc aatttttgct tttaattcac gacgacgacg gtgtaagact ggctcagtat
      421 aaccgtttgg ttgagcacaa ccttcaaaca ctaactcttt agacgcttgg aaagccacgc
      481 tgtcatcaaa gtttggtgcc attggtttgt ataatgggtc attagcgttt tgaccatcaa
      541 ccacagaggc catacgttgc atagcttcta acacttgttc ttgtgtacaa ataccatgac
      601 gtaaccagtt agctacgtgt tggctggaaa tacgcaaggt tgcacggtct tccattaagc
      661 caatgttgtt aatatctggc actttagaac aacccacacc ttggtcaatc caacgaacca
      721 cataacctaa aatgccttgg caattgttat caagttcatt tttgatttct tcagcagacc
      781 aatcagtatt ttctgctaaa ggaatggtta agatgtcatc aacattagca cgagcacgag
      841 actttaattc gtcttggcga gcaaacacat ttactttatg gtaatgcata gagtgtaaag
      901 tcgcgcctgt tggtgatggt acccaagccg tatttgcgcc agccattggg tgagcaattt
      961 tttgctcaac catgtctttc attaagtctg gcattgccca catgccttta ccgatttgtg
     1021 ctttaccttg taaaccacaa gctaaaccga tatcaacgtt ccagttttcg taggaagcaa
     1081 tccatttagc ttttttcatg tcagctttac gaatcaccgc acctgcttcc atagaagtat
     1141 ggatttcatc accagtacga tctaagaagc ccgtgttaat gaacatcaca cgatctttag
     1201 cagcacggat acattctttg aggttaacag tggtacgacg ctcttcgtcc ataataccca
     1261 tttttaaggt attttgtgct aaacctaaag cttcttctac acgagtgaag atttcgttag
     1321 caaacgcaac ttcttctgga ccgtgcattt ttggcttaac aatatacaca gaacctgtac
     1381 gagaattctt taaagagcca ttgcccaata aatcgtgttt tgcaatcaat accgtcacta
     1441 aggcatccat aatgccttct ggaatttctt cacctttata caatacggct gggttagtca
     1501 ttaaatgacc aacgttacgc actaataata aagaacgacc atgcaaagtg aggctgccac
     1561 catttaaagc agtgtaagta cgatcagcat tcatggtacg agttaaggtt ttgccacctt
     1621 tttcgaaaga ctcaaccaag ttgcctttca ttaaacccaa ccagttgcca tagacttcaa
     1681 ctttgtcttc agcgtcaacg gcagccacag agtcttcaca gtcacaaata gtggttaacg
     1741 cagactcaat tactaaatct ttgatttttg ctgggtcttg ttgaccaaca ggatgattgc
     1801 catcaaattg aatatcaatg tgcaaaccgt tgttttttaa taaaatgcta gttggcgcac
     1861 taagatcacc agcataaccc actaattttt ctggggatgc taaaccaact ttgctgccat
     1921 cttttaagct aacttctaat gcaccattaa caatagcata agcgacggaa tcggcatgag
     1981 agccagttgc caaaggagcg gctgtgtcta ataaagtacg agcataagca ataactttag
     2041 caccacgagc tgggttgtag cctttgcctt tgtctgcacc accttcttcg ctaatcacat
     2101 cgttgccata caaggcatcg tataagctac cccaacgagc attagctgcg tttaaggcat
     2161 aacgcgcatt tttaacaggc acaactaatt gtgggccagc aatacgggca atttcgtcat
     2221 ctacgttttg tggattaacg ctaaaatctt cgccttctgc gactaaatag ccaatttctg
     2281 ttaaaaaggc tttgtaagca gcaaagtcaa attttggatt agcttgatgc catgcgtcaa
     2341 tttgcgcctg aattgcatca cgcttggcta ataaagcttt gttttttggc attaaatcag
     2401 caacaatcgc ctcaaactta gtccagtaat catcaacgct gattccagta ccttcaatcg
     2461 cttgattaac aataaaatca tacaattctt tagcaatcgc taactcacca cgttgaacac
     2521 gttcggtcat cttgtcaccc tttaatcaat aagccacgcc aa
//
LOCUS       DAOQMB010000335         2559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24652_length_2559_cov_12.904553, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000335 DAOQMB010000000
VERSION     DAOQMB010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24652_length_2559_cov_12.904553"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PLY05_09575"
     CDS             <1..243
                     /locus_tag="PLY05_09575"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNC03586.1"
                     /translation="LERWGARTLDLDILLYDQQVIDTEQLIVPHKELVNRCFVTLPLL
                     DINPTLTLPNGVKVSSLACATLSDDIYVLADNCHWY"
     gene            357..1148
                     /gene="panB"
                     /locus_tag="PLY05_09580"
     CDS             357..1148
                     /gene="panB"
                     /locus_tag="PLY05_09580"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNC03587.1"
                     /translation="MPITLTTLRDCKKRGEKFAMITCYDTIFAQTMNAAGVECLLVGD
                     SLGMVLQGHSSTLPVTMQDMIYHTQCVARGNNNAFLLADMPFMSYATVNDALNNAAAL
                     MRVGAHAVKLEGGAWLADTVTQLARGGIPCCVHMGLTPQSVHVFGGYKVQGKTADAAA
                     KLIEEAKIVEQAGAALILLECVPSAVAAQVTASVSIPVIGIGAGKDCDGQVLVVHDML
                     GLHQGKAARFVKNFLTGQASIQDAFVAYVKAVKEQSYPAPEHGFN"
     gene            1150..2004
                     /gene="panC"
                     /locus_tag="PLY05_09585"
     CDS             1150..2004
                     /gene="panC"
                     /locus_tag="PLY05_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNC03588.1"
                     /translation="MQTVHTIQAVREFVNAAKQQGKKVGFVPTMGNLHAGHIHLIAQA
                     KTQADVVICSIFVNPTQFGANEDFGSYPRTLEADSQQLQAAACDLLFAPSAQEMYQNG
                     FSQHTVVTVNGLSEQLCGQFRPTHFAGVATVVSKLFNIVQPDMAFFGEKDFQQLAVIR
                     CLTTELAFPIEIVGVATQRAEDGLALSSRNGYLSAEERAKAPMIYQMLQQLKTAIDNG
                     HRDYTMLCDAAMLHLQKSGFDPDYIAVKRAIDLQDANADDLDLVLLVAARLGKTRLID
                     NIAFKLDN"
     gene            2189..>2559
                     /locus_tag="PLY05_09590"
     CDS             2189..>2559
                     /locus_tag="PLY05_09590"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004990637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HNC03589.1"
                     /translation="MQCTMLKAKLHRAVVTHAEVDYEGSCAIDGNLLDLSGILEYEQI
                     QIYNVTNGERFTTYAIRAEAGSRIISVNGAAAHKAQPGDKVIICAYANYAAAELINFK
                     PRLIYCNPDNTVSHSANAIPV"
BASE COUNT          713 a          549 c          550 g          747 t
ORIGIN      
        1 cttgaacgtt ggggcgcacg caccttagat ttagatattt tattatatga ccaacaagtc
       61 attgataccg agcaactgat tgttccacat aaagaattag tcaatcgttg ttttgtgact
      121 ttacctttat tagatataaa ccctaccctt acactgccaa atggtgtaaa agtgtctagc
      181 ctagcctgtg ctaccctatc cgatgatata tatgtacttg cagataattg ccattggtat
      241 tagccctcac cccaaccctc tcccacaggg cgagggagtt ctctctccct ttgggagaga
      301 gctagagtga ggggaaatta acaaattgtg cttttatctt aattatgagg ttatcaatgc
      361 caatcaccct aacgacactc cgcgactgca aaaaacgtgg cgaaaaattt gccatgatta
      421 cctgttatga caccattttt gcccaaacaa tgaatgcggc tggtgtggaa tgcttattag
      481 tcggcgactc tttaggcatg gtcttacaag gccatagctc aacgctacct gtgactatgc
      541 aagacatgat ttatcatact caatgtgtgg cacgcggcaa caataatgcc tttttattgg
      601 ccgatatgcc gtttatgtct tatgccaccg ttaatgatgc cctaaacaac gcggcagcat
      661 taatgcgtgt tggtgctcat gcggttaagt tagaaggtgg tgcttggtta gctgacactg
      721 tcacccaatt agcccgtggt ggcattcctt gttgtgtcca catgggctta acgcctcaat
      781 ctgtgcatgt ttttggtggc tataaagtac aaggtaaaac ggctgatgct gctgctaaat
      841 taattgaaga agccaaaatt gttgagcaag caggtgcggc attgattttg ctcgaatgcg
      901 tacccagtgc tgttgccgca caagtcacag cgagcgtctc tattcctgtc attggtattg
      961 gtgcaggcaa agactgtgac ggtcaagttt tagttgttca cgatatgtta ggcttgcacc
     1021 aaggcaaagc tgcgcgtttt gtcaaaaact ttttaacagg ccaagccagc attcaagatg
     1081 cttttgtagc ttatgtgaaa gccgtaaaag agcaaagcta ccctgcaccc gaacatggat
     1141 ttaattaaca tgcaaactgt acataccatt caagccgtgc gtgagtttgt taacgctgcc
     1201 aaacaacaag gtaaaaaagt gggctttgtg ccaacgatgg gcaacttgca tgcaggtcat
     1261 attcatctaa ttgctcaagc caaaacacaa gccgatgtgg tgatatgcag catttttgtc
     1321 aatcccacgc agtttggtgc caatgaagac tttggcagtt atccacgtac cttagaagct
     1381 gatagccaac aattacaagc ggcggcttgt gatttgctct ttgccccttc tgcacaagaa
     1441 atgtatcaaa atggctttag ccaacatact gtggtgacag tgaatggctt aagtgaacaa
     1501 ctttgtggtc aatttagacc aacgcatttt gcaggtgttg ctactgttgt cagtaagcta
     1561 tttaacattg tacaacctga catggcattt tttggcgaaa aagactttca acaattagcc
     1621 gtgattcgtt gtttaactac tgagttagct tttccgatag agattgttgg tgttgccact
     1681 caacgtgccg aagatggcct agcactgagt tcacgtaatg ggtatttatc tgccgaagaa
     1741 cgtgctaaag ctccaatgat ttatcaaatg ttgcagcagc taaaaactgc aattgacaat
     1801 ggtcatcgtg attacaccat gttatgtgat gcagcgatgt tgcatttaca aaaatctggc
     1861 tttgatcccg attatattgc tgttaaacgt gcaattgacc tacaagatgc caatgcagat
     1921 gacttagact tggtattatt ggtggctgcg cgtttaggca aaacccgttt aattgataat
     1981 attgcgttta aattagacaa ttaagcagca aaatttcgga caatcgtcca ataagttatt
     2041 tttattacag atataggttg aatcggggac aagattacgg caaaatgacg cgcccatttt
     2101 tatgactatt tataatggtt gaagttataa ataatcagcg taaaatgtcc cgaacaagcc
     2161 ctgcgacccc cctacggagg caaccgccat gcaatgcacc atgcttaaag ccaaactcca
     2221 ccgtgctgtt gtgactcacg ctgaagttga ttatgaaggc tcttgcgcca ttgacggtaa
     2281 cttgctcgat ttatcgggca ttttagagta tgaacaaatt caaatttaca acgtcaccaa
     2341 tggtgaacgt tttaccactt acgcaattcg tgccgaagct ggctcacgta ttatttccgt
     2401 taacggtgcg gctgctcaca aagcccaacc tggtgacaaa gtgattattt gtgcctatgc
     2461 caattatgca gctgccgaat taattaactt taaacctcgc ttaatttatt gcaacccaga
     2521 caatactgtt agccatagtg ccaatgctat tcctgtgca
//
LOCUS       DAOQMB010000336         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24768_length_2553_cov_5.515212, whole genome shotgun sequence.
ACCESSION   DAOQMB010000336 DAOQMB010000000
VERSION     DAOQMB010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24768_length_2553_cov_5.515212"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLY05_09595"
     CDS             complement(<1..240)
                     /locus_tag="PLY05_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03590.1"
                     /translation="MMTKTMRSVIIADAGPLIHLDELGCLDVLTCYPEVLLPEAVWQE
                     VMQHRPQALQHSLVNFKQQQLKIISLQVEALVPLYT"
     gene            complement(230..424)
                     /locus_tag="PLY05_09600"
     CDS             complement(230..424)
                     /locus_tag="PLY05_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03591.1"
                     /translation="MSMIHAEVPDGLLQQAQLLVQRGWANNIQELVNESLRRYIESHQ
                     EALTEQFIREDVEWGLHGDD"
     gene            complement(470..2026)
                     /locus_tag="PLY05_09605"
     CDS             complement(470..2026)
                     /locus_tag="PLY05_09605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HNC03592.1"
                     /translation="MNIIELERTLWATADKLRANMDAAEYKHIVLGLIFLKYISDTFT
                     ARRAELLKRFVDENDEYYFADATPEELQAELEDRDYYREVNVFWVPEAARWDVLLAAA
                     KQPDIGKKIDDALAVIESENPKLKGILDKRYARAQLPDGKLGELVDLVSTIGFGADAS
                     QARDILGQIYEYFLGQFASAEGKRGGQFYTPASIVKTLVAVLAPHSGKVYDPCCGSGG
                     MFVQSEKFIEAHGGKLGDVSIYGQESNPTTWRLAAMNLAIRGIEFNLGREPADTFTRN
                     QHADLRADFILANPPFNISDWWHGSLMGDSRWEYGDPPQGNANFAWLQHMLYHLKPTG
                     RAGIVLANGSMSSTQNSEGQIRQAMIEADVVEVMVALPSQLFFNTQIPACLWFLAKQK
                     TTRKGEVLFIDARKLGQMISRAQREMTDADIALIGDTVAAWRGEAGAKDYADVAGFCR
                     SVPLAEIAEHGHVLTAGRYVGAEEVEDDDEAFADKMQKLTEKLAEQMAKGAELDQVIR
                     QKLAGLGYEF"
     gene            complement(2131..2343)
                     /locus_tag="PLY05_09610"
     CDS             complement(2131..2343)
                     /locus_tag="PLY05_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03593.1"
                     /translation="MDSENKQRLRGLMLNANLDTLAVKQLLEQQTKRKYSIRTVQAWA
                     ADSSKASSRECPEWVLENLEQIVKGL"
     gene            complement(2336..>2553)
                     /locus_tag="PLY05_09615"
     CDS             complement(2336..>2553)
                     /locus_tag="PLY05_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03594.1"
                     /translation="LVLQQNKGKIEHAQVEKKTIKADSMQLLQQGKKLIVAVKSRKAS
                     YDIELPIAAQQLQELLQQLLKESYVDG"
BASE COUNT          689 a          625 c          487 g          752 t
ORIGIN      
        1 ggtgtataac ggcactaagg cttccacttg taacgaaatg atctttaatt gttgttgttt
       61 aaagttaacc aaagaatgtt gtaaagcctg tggccgatgc tgcataacct cttgccaaac
      121 ggcttcaggc aacaacacct cggggtagca ggtcaataca tctaaacaac ccaattcatc
      181 taaatgaata agtggacctg catcagcgat aatcaccgac cgcatagttt tagtcatcac
      241 cgtgtaagcc ccactccaca tcttcacgaa taaactgttc ggttaatgcc tcctgatgag
      301 actcaatata acgccgtaaa gactcattaa ctaactcttg aatgttatta gcccagcctc
      361 gctgtactaa taattgtgct tgctgcaaca agccatcggg aacttccgca tgaatcatgc
      421 tcatgattgt ctcctcaata tattttctta aatcaaaatt aatgtatgat taaaattcat
      481 acccaagccc tgccaacttc tgcctaatca cttggtctag ctctgcccct tttgccattt
      541 gctcggctaa tttttcggtc aatttttgca ttttatcggc aaaggcttca tcgtcatcct
      601 ccacctcctc cgcacccaca taacgtcctg cggtcaacac atggccatgc tcggcaattt
      661 ccgccaaagg cacactacga caaaagccag caacgtctgc ataatctttt gcccctgcct
      721 caccacgcca agcagccacc gtgtcaccaa ttaacgcaat atcggcatcg gtcatttccc
      781 gttgagcacg actaatcatc tgccctaatt tacgcgcatc aataaacagc acttcgcctt
      841 tacgtgtggt tttttgtttg gctaaaaacc acaaacaagc aggaatttgg gtgttaaaaa
      901 ataattgact tggcaaagcc accatcactt ccaccacatc ggcttcaatc attgcttggc
      961 gaatttgccc ttcgctattt tgtgtcgaac tcattgaacc attggccaac acaatccctg
     1021 cgcggcctgt cggtttaagg tgatacaaca tgtgttgtaa ccatgcaaag ttggcgttgc
     1081 cttgtggtgg gtcgccatat tcccaacgtg aatcacccat taagctaccg tgccaccaat
     1141 cactaatatt aaacggtggg ttggccaaaa taaaatcagc gcgtaagtca gcgtgttgat
     1201 tgcgggtaaa ggtgtcagca ggttcgcggc ctaaattaaa ctcaatgccc cgaatggcaa
     1261 ggttcatggc agccagtcgc catgtggttg ggttggactc ttggccataa atcgacacat
     1321 cacccaattt gccgccgtgt gcttctataa atttttcgga ctgaacaaac atgccacccg
     1381 aaccacaaca cgggtcatac actttgcctg aatgtggcgc aagcacggcc actaaggttt
     1441 ttacaatact ggctggggta tagaactgac cgccgcgttt gccttcggca ctggcaaatt
     1501 ggcctaaaaa gtattcataa atttggccta aaatatcacg cgcttgactg gcatctgcac
     1561 caaaaccaat ggttgacact aaatcaacca attcgcccaa cttgccgtca ggcaattgcg
     1621 cacgcgcata acgcttgtct aaaatacctt taagtttggg gttttcgctt tcaataacgg
     1681 ctaaggcatc atcaattttt ttgccaatgt caggctgttt agccgcagct aataacacat
     1741 cccaacgtgc tgcttcggga acccaaaaca cattgacttc gcggtaataa tcgcggtctt
     1801 ctaattctgc ttgcagctct tcgggtgtag catcggcaaa ataatactca tcgttttcat
     1861 caacaaatcg tttgagcaat tcggcacgac gtgcggtaaa agtatcagaa atatatttta
     1921 aaaaaattaa gcctaaaaca atgtgtttat attcggcggc atccatgtta gcgcggagtt
     1981 tatcggcagt tgcccataag gtgcgttcta gttcgatgat gttcatacta tcccttaacc
     2041 tatttattgc gtaaaaataa ctatgacgat gtttaatgtg gacgactaaa atcaagtaat
     2101 ttataacaat tgagcatgct caatttttgc ttaaagtcct ttaacaattt gctctaagtt
     2161 ctctaaaacc cattctggac actctctgga gcttgctttt gacgagtcag ccgcccacgc
     2221 ttgcacggtt ctaatactgt atttacgttt ggtttgctgt tctaggagtt gtttaacggc
     2281 caaagtatct aaattagcat taagcattaa accgcgaagt ctttgtttgt tttcgctatc
     2341 catcaacgta actttccttg agtagttgtt gtaataactc ttgtaattgt tgtgctgcta
     2401 ttggcaactc tatgtcataa ctggccttac gcgatttaac cgcaacaatc aattttttac
     2461 cttgttgtag caattgcatg ctatcagctt tgatggtttt tttctcgact tgagcatgct
     2521 caattttgcc tttgttttgt tgcaaaacca gat
//
LOCUS       DAOQMB010000337         2546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24875_length_2546_cov_17.282216, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000337 DAOQMB010000000
VERSION     DAOQMB010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24875_length_2546_cov_17.282216"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PLY05_09620"
     CDS             complement(<1..327)
                     /locus_tag="PLY05_09620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC03595.1"
                     /translation="MVSPQSITLRIMHTDDLAAVCAIEKQVQYAPWSEKLFAEGLERH
                     RCMVAINQQQQIVGFSIVQFIVDEAHLLNIAVEPTQQKQGIGKVLLDDVLVNSQQKKA
                     STIFLEV"
     gene            complement(306..1109)
                     /locus_tag="PLY05_09625"
     CDS             complement(306..1109)
                     /locus_tag="PLY05_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03596.1"
                     /translation="MSNIAVEGRRQAYLAALGIPVWTVRAESQLADVSDPVAFVPYYL
                     EQLIDEQSISYQPHKEQAVTVVEQELPKPIVKQPVHSFDEPPLESYLNDPEYQRAEEF
                     DDFVRARTALTEDVKPTPTEQTELQQRVVVNDTQPITFRFAVYGCGAWQLIVPRSQLL
                     SPSEMTLLANIQRVMQSENAPPLLFVWPMVHNYALPRHRQAAQEALAFFFRNQALSDK
                     GYIVLTDHEQDLVDLLRASTDKTVIQQASLSLLLQQPLQKKALWLALNQ"
     gene            complement(1113..>2546)
                     /locus_tag="PLY05_09630"
     CDS             complement(1113..>2546)
                     /locus_tag="PLY05_09630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNC03597.1"
                     /translation="MKVDVIEAGFAIASHGDFLAVKAVAEAVKDSRICSLARARAEDI
                     DRAAEALKNANAARIHTFIATSPIHMKYKLRLEPDAVVENAVRAIKHARNWVEDVEFS
                     CEDAGRSELDFLCRIIEAAIDAGARTINIPDTVGYAIPQQFGELIHNIITRVPNADKA
                     IFSVHCHNDLGLAVSNSLAAVLNGARQVECTINGLGERAGNAALEEVVMAIRTRSDLF
                     PVETGIYTPEIVPTSRLVSGITGFPVQPNKAIVGANAFAHESGIHQDGVLKHRETYEI
                     MRAEDVGWNANRMVLGKHSGRAAFKARLEQLGIDAGTDNSFNSLFMRFKELADKKHDI
                     FDEDLHALVSDSQNEVAETYKLNSLEVTSKTGTKPEAKLTLVVNGQEKQVTAIGSGPV
                     DATFRAIEAVADSGTTLELYSVNAITSGTDSQGEVTVRLNQAGRIINGHGADTDIVIA
                     SAKAYLDALNLMVANVARAHPQEEDNI"
BASE COUNT          752 a          588 c          488 g          718 t
ORIGIN      
        1 cacttctaaa aaaatagtgc tggctttttt ttgctgacta ttaactaata catcatctaa
       61 taaaacttta cctatgccct gcttttgctg tgtgggttca acagcaatat ttaacagatg
      121 cgcttcatcg acaataaatt gaacaattga gaaaccaacg atttgctgtt gttgattaat
      181 tgctaccata caacgatgac gctctagccc ttcagcaaat aatttttctg accaaggggc
      241 gtattgaact tgtttttcga tagcacacac agcagctaaa tcatctgtgt gcataatgcg
      301 taaggttatt gattgagggc taaccataag gcttttttct gtagaggttg ctgtaataac
      361 aaacttaaac tagcttgctg aataaccgtt ttgtcagtgc tggcacgtaa taaatccact
      421 aaatcttgct cgtgatcggt taaaacaata taccccttat cacttaaggc ttgattacga
      481 aagaaaaaag ctaaagcttc ttgcgctgcc tgacgatggc gtggcaaggc atagttatgc
      541 accattggcc aaacaaacaa taaaggtggt gcattttcac tctgcatcac tcgttgaata
      601 ttcgccaaca aagtcatttc actgggactt aataattgtg aacgcggcac aatcagttgc
      661 caagcaccac aaccatatac agcaaaccga aaagtaatgg gttgtgtatc gttaacgaca
      721 actcgttgct gtaactccgt ttgttcggtc ggtgtgggct taacgtcttc agttaatgct
      781 gtacgcgcac gaacaaaatc atcaaactct tcagcacgtt gatattcagg atcatttaaa
      841 taactttcta aagggggctc atcaaagctg tgaacgggtt gcttaacgat tggtttgggc
      901 agctcttgct caacaacagt gactgcttgt tctttatgcg gctgataact gattgattgc
      961 tcgtcaatca gttgctctaa ataatacggc acaaaagcaa caggatcact cacatcggca
     1021 agttggcttt cggctctgac tgtccagact ggaatgccta atgccgccaa ataagcttgg
     1081 cggcggcctt caacggcaat attgctcata aattaaatat tatcttcttc ttgaggatgg
     1141 gcgcgtgcga cattagccac catcaagtta agtgcatcta aataggcttt ggcactggca
     1201 atgacaatgt cggtgtccgc accatgacca ttaataatac ggccagcttg attcagacgc
     1261 accgtcactt caccttgaga gtctgtgcca ctggtaatcg cattcaccga atacaattct
     1321 aatgttgtac cagagtctgc aactgcctca atcgctctaa aggtcgcatc aacagggcct
     1381 gagccaatcg ctgttacttg cttttcttga ccattgacca ctagtgttaa ttttgcttca
     1441 ggtttggtgc ctgttttaga agtgacttct aaactattga gtttgtaagt ttcagccact
     1501 tcgttttggc tatcactgac taaagcatgc aaatcttcat caaagatatc atgttttttg
     1561 tcagccaatt ctttaaaacg cataaacaag ctattaaagg aattgtcagt gcctgcatca
     1621 atgcctaatt gctccaagcg tgctttaaaa gcggcacgac cagaatgttt acctaaaacc
     1681 atacgattgg cattccaacc cacatcttcg gcacgcataa tttcgtaagt ttcacggtgt
     1741 tttaacacac catcttggtg aatacctgat tcatgcgcaa aagcatttgc acccacaatc
     1801 gctttattag gctgcacagg aaagcctgta ataccagaca ctaaacgtga ggtgggtaca
     1861 atttcagggg tataaatacc tgtttcgaca ggaaataaat cactacgggt acgaatggcc
     1921 atcaccactt cttctaaagc agcattgcct gcacgctcac ccaaaccgtt aatggtacat
     1981 tcaacttgac gtgcaccatt taacacagcc gccaaagaat ttgataccgc taaacccaaa
     2041 tcattatggc aatgtaccga aaaaatcgct ttgtcagcat tagggacacg agtaataatg
     2101 ttatgaatca gctcaccaaa ttgctgagga atcgcataac ctacggtatc aggaatatta
     2161 atggtacgcg cacccgcatc aatggctgct tcaataattc gacataaaaa atctaattcc
     2221 gaacgtcctg catcttcaca agaaaactcg acatcctcaa cccaattacg tgcatgttta
     2281 atcgcgcgta ctgcgttttc aactacggca tcgggttcta aacgcaattt gtatttcatg
     2341 tgaatcgggc tagtagcaat aaaagtatga atacgtgccg cattagcatt ttttaaggct
     2401 tcggcagcac gatcaatatc ttctgcacgc gcacgcgcca acgaacaaat acgcgaatct
     2461 tttaccgctt cagcaacggc tttaactgcc aaaaaatcac catgactggc aatggcaaaa
     2521 cctgcttcaa taacatcaac tttcat
//
LOCUS       DAOQMB010000338         2539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_24983_length_2539_cov_12.936393, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000338 DAOQMB010000000
VERSION     DAOQMB010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24983_length_2539_cov_12.936393"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /gene="zapE"
                     /locus_tag="PLY05_09635"
     CDS             complement(<1..643)
                     /gene="zapE"
                     /locus_tag="PLY05_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNC03598.1"
                     /translation="MSPQSVKLLSPLARYQQDLATGTFQPDGAQLAAVQELQRIYDEL
                     VDRYQATQKSKGLGRIKLAKRLQQPVLGLYLWGGVGRGKTYLMDLFFEALPFRRKMRM
                     HFHRFMQRVHKELNEFRGEKNPLELVADRIHADAVVICFDEFFVTDITDAMLLKGLFE
                     QLFARGVSLVATSNIVPDRLYENGLQRERFLPAITLVKDNCQVLNVDSGIDYRL"
     gene            complement(640..1257)
                     /locus_tag="PLY05_09640"
     CDS             complement(640..1257)
                     /locus_tag="PLY05_09640"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03599.1"
                     /translation="MSATSYVQGQAGRIEVRLLMPQHAKANVFAVVCHPHPLYGGTMD
                     NKVVTTLTRTFKDLAIPVVSFNFRGVGQSEGLHDYARGEVDDLLAVIAWAKQQTQAEK
                     LYLAGFSFGSYVAAVAASRLSHVQLQQLLLVAPPVHHYAFNELALPIEQTLVIQGDAD
                     EVVPAQEVFDWAAQQQITVLKFPNCSHFFHGRLVELRQAVLENLV"
     gene            1362..1808
                     /locus_tag="PLY05_09645"
     CDS             1362..1808
                     /locus_tag="PLY05_09645"
                     /inference="COORDINATES: protein motif:HMM:NF018049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HNC03600.1"
                     /translation="MEWLLYIGLIAIGFIGGYVFQIYRNSDSRVRELESHLHSLQGKY
                     ESYQESVTSHFSHSAQLVNNLTNAYREVHEHLQKGAHELCADNKRHTSNNPATAFLGL
                     EATKESFPQAAGSYENKFLEPLEPPRDYAPKSANDKGMLDEEYGFK"
     gene            1860..2093
                     /gene="vapB"
                     /locus_tag="PLY05_09650"
     CDS             1860..2093
                     /gene="vapB"
                     /locus_tag="PLY05_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNC03601.1"
                     /translation="METAKLFHSGRSQAVRLPKAFRFSGEEVAIKHFGNGVLLLPLQN
                     PWQLMQDALDEFEPEFLLERETQTEQLREDLQF"
     gene            2090..2485
                     /locus_tag="PLY05_09655"
     CDS             2090..2485
                     /locus_tag="PLY05_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03602.1"
                     /translation="MIYLLDTNICIYIINNKPLHVFQRFQKYQLNEIAISSISAAELA
                     FGVQKSGSLRNRQALEKFLAPLDILPFNEECIWQYAQLRTQLQQQGKPIGSLDMLIAA
                     HALSINATLVTNNTQEFERIQGLNLENWI"
BASE COUNT          797 a          515 c          481 g          746 t
ORIGIN      
        1 gcaaacgata atctatacca gaatcaacat ttaatacctg acaattatct ttgactaaag
       61 taatagcagg taaaaaacgt tcgcgctgca aaccattttc atataaacgg tctggcacaa
      121 tattactggt ggccaccaaa ctgacaccac gggcaaataa ttgttcaaat aaacctttta
      181 ataacatcgc atcggtaata tcagtaacaa aaaactcatc aaaacaaatc accactgcat
      241 cggcatgaat acggtctgcc actaactcta aaggattttt ttcgcctcta aactcgttaa
      301 gttctttgtg tacacgctgc ataaaacggt gaaagtgcat ccgcatcttg cgtctaaagg
      361 gtaaggcttc aaaaaataaa tccattaaat aagttttgcc tcgccctacg cccccccata
      421 aatacaaacc caatacaggt tgttgcaaac gtttggctaa tttaatccgt cccaagcctt
      481 tgcttttttg tgtggcttga taacggtcaa ctaattcatc ataaattctt tgtaactctt
      541 gtacggcggc caattgtgcg ccatctggct gaaatgtgcc tgtcgctaaa tcctgttgat
      601 aacgagctaa tgggcttaat aacttaacag attgtggact catacaaggt tttccaatac
      661 ggcttggcgt aactcaacta aacgaccatg aaaaaaatgc gagcaattag ggaattttaa
      721 caccgttatt tgttgttgtg ctgcccaatc aaagacttct tgtgcaggca caacttcatc
      781 agcatcccct tgaatgacca aagtttgttc tatgggcaat gctaactcat taaaggcata
      841 atgatgcaca ggtggtgcaa ctaacaataa ttgttgtaat tgcacatgac tcaaacgact
      901 tgcagccaca gcagccacat aagaaccaaa agaaaagcct gctaaataga gcttttcggc
      961 ttgcgtttgt tgtttagccc atgcaataac agctaataag tcatccactt caccgcgtgc
     1021 atagtcatgt aagccttcac tttggccaac tccacgaaag ttaaacgaaa ccacaggaat
     1081 ggctaaatct ttaaaggtgc gggttaaggt ggtaacaact ttgttgtcca ttgtgccacc
     1141 atataaagga tgaggatgac aaacgacagc aaatacatta gctttggcgt gttgtggcat
     1201 taataaacgc acttcgatgc gacctgcttg accttgtaca tagcttgttg cagacatttg
     1261 cagtctccta cagtattttg atggcgaatg ttatagacta gtgtcgtcaa tgccaagaca
     1321 agcaagacaa aattcagtcg atagtgagat aagggggttt tatggagtgg ctattgtata
     1381 ttggcttaat tgctatcggt tttatcggtg gctacgtttt tcagatttac cgtaatagcg
     1441 atagccgagt acgcgaatta gaaagccatt tacattcatt acaaggcaag tatgaaagct
     1501 atcaagaatc cgtgactagc catttttctc attctgcaca attggtcaat aatttaacca
     1561 atgcctaccg tgaagtgcat gagcatttac aaaaaggcgc acatgagctt tgtgccgaca
     1621 ataaacgtca taccagtaat aatccagcta cggccttttt aggtttagaa gcgactaaag
     1681 agtcttttcc tcaagcggct ggctcttatg aaaacaaatt tttagagcct ttagagccac
     1741 ctcgtgacta cgcgcctaaa agtgccaatg ataaagggat gttagacgag gaatatgggt
     1801 ttaaataata ttgtgctaca atggtaatta ccactgataa ctaccaaata tcaatcatca
     1861 tggaaacagc aaaacttttt cattctggtc gtagccaagc agttcgtttg cccaaagcat
     1921 ttcgtttttc tggcgaagaa gttgccatta aacattttgg caatggtgtg ttactgttgc
     1981 ctttacaaaa tccttggcaa ttaatgcaag atgctcttga tgaatttgag cctgagtttt
     2041 tgttagaaag agaaactcaa accgaacaac tgcgagagga tttgcagttt tgatttatct
     2101 tttagatact aacatttgca tttatattat caataacaaa ccattacacg tctttcagcg
     2161 ttttcaaaaa taccaattaa atgaaattgc tatttctagc attagtgcgg ctgaattagc
     2221 ttttggtgtg caaaaatcag gttctttacg caatcgtcaa gctttagaga aatttttagc
     2281 tccactggat attttgccgt ttaatgaaga gtgtatttgg caatatgctc aattacgcac
     2341 acaactccaa caacaaggta aacctatcgg cagtttggat atgttaattg ctgcacatgc
     2401 cttatccata aatgccacct tagtcaccaa taatactcaa gaatttgaaa gaattcaggg
     2461 gcttaattta gaaaactgga tataaaaaag gcggaaattt ccgccttttt tattggttaa
     2521 gccattactt tgcagagtg
//
LOCUS       DAOQMB010000339         2536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25058_length_2536_cov_2.973801, whole genome shotgun sequence.
ACCESSION   DAOQMB010000339 DAOQMB010000000
VERSION     DAOQMB010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25058_length_2536_cov_2.973801"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1183)
                     /locus_tag="PLY05_09660"
     CDS             complement(<1..>1183)
                     /locus_tag="PLY05_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03603.1"
                     /translation="NAPTPLPPTAPSNAPTPLPPTARPNQQGAGSNNQSPNNGNSAGQ
                     GAPDTARINRQRDANGRFTRGDNAESAESNTQGRGNRDSRGRFTGGNGQNAAERSAVS
                     RITDSLKDLNNTLNVNANTDRIDPTIDAIKELGSIASVGIDAGKKALSVSNTLIAKPA
                     MALGRGIKGLFKPKTDAINSPVAWYKRIWLTLRQGNRQDQTQHAQEQRRLDELIRGQG
                     QRGSSDSSFLMMLGLGIAALLAAIKNIKFPSLDDIKDKLKGLGTDNQSPVPVLKFPKF
                     GGTIIQANPALKWLSETKVGQTVSKFLKRLPFISSAIEAAAGGINAVNIANDANLTEE
                     QKQRKQSENAGATAGAIGGGLGGAAAGAAIGTALIPIPILGTIIGGVIGGWLGTDAGR
                     IV"
     assembly_gap    1184..1283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1284..1612)
                     /locus_tag="PLY05_09665"
     CDS             complement(<1284..1612)
                     /locus_tag="PLY05_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03604.1"
                     /translation="MSDIRIDDDGFLVGDGEQQRTEAENVLMREIKADTTTIVNLLKG
                     TVKLQRETLKATQNNSSPKPNGGNNGGNGSNNQQPKVNLVHPNRAAPTPSNAPTPLPP
                     TAPSNAPT"
     gene            complement(1615..>2536)
                     /locus_tag="PLY05_09670"
     CDS             complement(1615..>2536)
                     /locus_tag="PLY05_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03605.1"
                     /translation="GIKSRDITAEDLEAFRKNIETVRKVYKQGITADQVLNMSLDIDL
                     ERAKKQIFTALPTRYKGNVLHFMTNASKESDVKQHYVTVKFLDLVAIATTPIPLLTAT
                     KQVARGYLAFDCDCGRHTFWYRYISTIGGFNAGRAETGFPKIRNPNLHGVACKHVLKV
                     MSVIDTALSMQAIKEMVKIARKSAKPIDELPENQLSAKEVKDAAKRQLEKAHHERNQV
                     RRASDDPVKQRRAKKEAERLAKASQDKVKQQREERKRIITSGSPMERLNLRIDLENRV
                     KTLQREYSLSANENLLDAIENLQNDINRLI"
BASE COUNT          525 a          573 c          506 g          832 t
ORIGIN      
        1 acaatacgac ctgcgtctgt acctaaccaa ccgccaataa cccccccgat aattgtcccc
       61 aatataggaa tagggattaa cgctgtacca atagccgccc ctgctgccgc accccctaaa
      121 ccaccaccga ttgcccctgc ggttgcccct gcattttcgg attgtttacg ctgtttttgc
      181 tcctctgtta aattagcatc attagcaatg tttaccgcgt ttatgccgcc tgctgctgct
      241 tcaatcgctg acgaaataaa gggcaagcgt tttaaaaact tgcttaccgt ttgcccgact
      301 ttggtctcgc ttaaccattt taaagctgga ttagcttgaa taatcgtgcc gccaaatttt
      361 gggaatttta gcactggtac aggtgattga ttgtccgtac ctaatccttt tagtttgtct
      421 ttaatatcgt ctaacgaggg gaatttaatg tttttaatgg ctgctaataa tgccgctatg
      481 cctaagccaa gcatcattaa aaaactgcta tcactactgc cgcgctgtcc ttgacctctg
      541 attaattcat ctaaacgtct ttgttcttgc gcgtgttgtg tttggtcttg acgattacct
      601 tgccttaacg tcaaccaaat acgcttatac caagctacag gcgaattaat cgcatcggtt
      661 ttaggtttaa acaatccttt aatgccacgg cctaatgcca ttgctggttt agcaatcaag
      721 gtattgctca cgcttaatgc ttttttacct gcatcaatgc ccacactcgc aatactgcct
      781 agctctttga ttgcatcaat agtcgggtct attctgtcgg tattagcatt aacatttaaa
      841 gtgttattta agtcttttag gctatctgtg atgcgtgata ccgcgctacg ctctgccgcg
      901 ttttgaccat tgccgcctgt aaaacgacct ctactgtcac gattgccgcg cccttgtgtg
      961 ttgctttctg cgctttcagc attatcaccc cttgtaaacc gcccattagc atcacgttgt
     1021 cgattaattc gtgcggtatc gggtgcgcct tgtcctgcac tgttaccgtt attaggcgat
     1081 tgattattgc tacctgcccc ttgttgatta ggccgtgctg ttggcggtaa aggtgttggc
     1141 gcgttacttg gtgctgtcgg tggcaaaggt gttggcgcgt tacnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnngttggcg cgttacttgg tgctgtcggt ggcaaaggtg
     1321 ttggcgcgtt acttggtgtt ggtgctgctc ggtttgggtg aacaaggttt acttttggct
     1381 gttgattgtt gctaccattg ccgccattat tgccgccatt aggttttggg ctactgttat
     1441 tttgcgttgc ttttaaggtt tcgcgttgta gcttaactgt gccttttaac agattaacaa
     1501 tggttgtagt gtcggcttta atctcgcgca ttagcacatt ttcggcctct gtgcgttgtt
     1561 gttcgccatc gcctactaaa aagccatcat cgtctatgcg tatatctgac atgattaaat
     1621 caacctgtta atatcattct gtaagttttc aatcgcatct aacaagtttt cgttagccga
     1681 tagactgtat tcgcgctgca aggtttttac tctgttttct aaatcaatgc gtaaattcaa
     1741 acgctccata ggcgaaccac tggtaataat gcgtttacgt tcctcgcgtt gttgcttaac
     1801 cttgtcttgt gacgctttcg ctaatcgctc ggcctctttt ttggctctac gttgtttaac
     1861 agggtcatcg cttgcacgtc ttacttggtt tctttcgtgg tgcgcttttt ctaactgccg
     1921 tttagccgcg tctttaacct ctttagccga taactgattt tcgggtaatt catcaatcgg
     1981 cttggcactt ttacgcgcta tttttaccat ttctttaatg gcttgcatcg acaaggcggt
     2041 atcaatcacg ctcattactt ttaaaacgtg cttacacgct actccgtgca aattagggtt
     2101 tctaattttt gggaatcctg tctcggcacg tcctgcatta aagccgccaa tggttgatat
     2161 gtacctgtac caaaaggtgt gtctgccaca atcacaatca aaggctaagt aacctcgcgc
     2221 cacttgctta gtagctgtca ataatggaat aggcgttgtt gcaatggcta ccaagtctaa
     2281 aaacttaaca gtcacataat gctgtttaac gtctgattct ttgcttgcat tcgtcataaa
     2341 atgcaaaaca ttacccttgt aacgtgttgg caatgctgta aatatctgtt ttttggcgcg
     2401 ttctaagtct atatctaaac tcatgtttag cacttggtcg gcagttatgc cttgtttata
     2461 gactttgcgt accgtctcaa tgttttttct aaaggcttct aaatcctcgg cagtaatatc
     2521 tcggctttta atgcct
//
LOCUS       DAOQMB010000340         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25062_length_2535_cov_9.486290, whole genome shotgun sequence.
ACCESSION   DAOQMB010000340 DAOQMB010000000
VERSION     DAOQMB010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25062_length_2535_cov_9.486290"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PLY05_09675"
     CDS             <1..746
                     /locus_tag="PLY05_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNC03606.1"
                     /translation="LYFTESLKSSLFVQQSHNDWKPFSFISKGKVQGIFIDLMRGGAE
                     KAGYKVNFKAMPWDVCLNQVKTGNLDGGVPASYKADRAEFMYYPDDAATNNDSKWAVG
                     KADYVVLVKVASKDGNYEYEGDPKTLPQPIFVPRSYSVADDLKKLGLQVSTNARYDEV
                     NLYQLLQSSGGSAVAAGVAVNSILENKFFTGKFKISRQSYTTKTYYMPMGKLAKTPKA
                     DMQKIWDGIAELRADNAWMRKTIAKYQKE"
     gene            840..2072
                     /locus_tag="PLY05_09680"
     CDS             840..2072
                     /locus_tag="PLY05_09680"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC03607.1"
                     /translation="MKKLSLLASLVSIFSINAAQAADADLQQRVSTLEAELAKLKAQP
                     VLTRVDGGLRAQSANGSSKFRFAGRVQADYAFYDKDVKDLGSGSELRTLRMGSRGTLA
                     KNWDYKMEADFTTGGTVRITEGYVAYTGMQNAVVQVGNIFEMYGLEEYSSSVDTTFME
                     RSVAVDAFVPDYNQGIGYIRWGDAYNFSAGIYGDSTNNAQAASNESAGTSARLVFAPL
                     HASGDVLSLGMSAYWRDVTSDAWRVRARPNSHVTPTRLVDTGNITDVSTATALALEAS
                     MTKGAFSVQAEYNQQMLNRNHGAKDADFKGWYIYGSYFLTGESRPWDMASATYGRPSP
                     KQAGGAWEVALRFDQLDLDDTGAGIKGGEMKTATLGLNWYPVNNVRFSTNLIKVDSEK
                     AGVEDNPNIIQMRAQVAF"
     gene            complement(2183..>2535)
                     /locus_tag="PLY05_09685"
     CDS             complement(2183..>2535)
                     /locus_tag="PLY05_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016652682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNC03608.1"
                     /translation="WAARFFKTRSLAKDAIEGGKVHCEGQRVKVSKEAKIGMELTIKQ
                     GFEDKTVVITALSEVRRGAPEAQLLYNETEDSIAKRQHLNEQRKAANLAYPDHRPNKK
                     ERRQIHKFQDSWRD"
BASE COUNT          725 a          463 c          596 g          751 t
ORIGIN      
        1 ctctttattt tacagaatcc ctcaaatcat cgctatttgt gcaacaaagc cataacgact
       61 ggaaaccgtt ctcttttatt tccaaaggaa aagtacaggg gatttttatt gatttaatgc
      121 gtggaggagc agaaaaagct ggttataaag ttaactttaa agcgatgccg tgggatgtgt
      181 gtttaaatca ggttaaaaca ggtaatttgg atggtggtgt acctgcttca tacaaagcgg
      241 atcgtgctga atttatgtat tatcctgatg atgctgccac taataatgat tctaaatggg
      301 cagtaggcaa agcagattat gtggttttgg ttaaggttgc cagtaaagat ggcaactatg
      361 aatatgaagg agatcctaaa actttaccgc aacccatttt tgtccctcgt agttattcgg
      421 tggccgatga cttaaaaaaa ctaggtttac aagtcagcac caatgcgcgt tatgacgagg
      481 ttaatttata tcaactatta caaagcagtg gaggatcggc ggttgccgca ggtgtggcgg
      541 tgaatagtat tttagaaaac aagtttttta caggcaaatt taaaattagc cgtcagtcat
      601 ataccaccaa aacctattat atgccaatgg gtaaattagc caaaacgcct aaagcagata
      661 tgcaaaaaat ttgggatggt attgcggagt taagagcaga taacgcgtgg atgcgtaaaa
      721 cgattgctaa atatcaaaaa gaataaaaca gatattgcgt gataaatcgc ggatttgtca
      781 cgcttgtatt aggcattatt aatcaatgcc tacatatttt aataacgagg aaaataacaa
      841 tgaaaaaatt gtccctatta gctagtttgg tgagtatctt ttctattaat gcagcacaag
      901 ccgccgatgc tgatttgcag cagcgagtga gcacattgga agcagaacta gccaaattaa
      961 aagcacaacc tgtgttaacg cgtgtcgatg gtggtttacg cgctcaatca gccaacggca
     1021 gcagtaaatt tcgttttgct ggtcgggtac aggctgatta tgctttttat gataaagacg
     1081 tcaaagattt ggggagtggc agtgagttgc gtaccttacg tatgggttca agaggcacat
     1141 tagctaaaaa ttgggattat aaaatggagg ctgattttac cacaggtggt acagtccgta
     1201 ttaccgaagg ttatgttgca tatacaggta tgcaaaatgc agtggtgcaa gtgggtaata
     1261 tttttgagat gtatggttta gaagagtaca gcagctctgt agatacgacc tttatggagc
     1321 gttcggttgc ggttgatgcc tttgtgccag attataatca aggcattggt tatattcgtt
     1381 ggggcgatgc ctacaatttc agtgcaggta tttatggtga ttcaacaaat aatgcccaag
     1441 ctgcaagcaa tgaatcagca ggaacttctg cacgtttagt ctttgcacca ttacatgctt
     1501 ctggtgatgt tctttcctta ggtatgagtg cttattggcg tgatgtaacc agtgatgctt
     1561 ggcgtgttcg tgccagacca aattcacatg tgacgcccac acgtttggtt gatacaggta
     1621 atattaccga tgtctcaact gcaacagccc tggccttaga agcctcaatg accaaaggtg
     1681 cattctcggt acaagcagaa tacaatcagc aaatgcttaa tcgtaatcat ggtgctaaag
     1741 atgcagactt taaaggttgg tacatttacg gcagttactt tttaacaggt gaaagtcgtc
     1801 cttgggatat ggcttcggca acttatggcc gtcctagtcc taaacaagct gggggcgcat
     1861 gggaagtcgc tttacgtttt gatcagctag accttgatga tacaggggca gggatcaaag
     1921 gcggagaaat gaaaacggca acgttgggtt taaattggta tcctgtcaat aatgtccgct
     1981 ttagtaccaa tctgattaag gttgattcag aaaaagcggg tgtagaagat aatcccaata
     2041 ttatccaaat gcgtgcccaa gtggcgtttt aacaaagctt atttttggtt tcgactccgc
     2101 tcaaccaacg caaaaatgcg ccctgagcgg agtcgaaggg taggctgtgt tctttaaatg
     2161 aatggctgta ggagggtact tatcagtctc gccatgaatc ttgaaactta tgaatctgtc
     2221 gtcgttcttt tttgttcggg cggtggtcag gataggctaa gtttgcggct ttacgctgtt
     2281 catttaaatg ctgacgtttc gcaatactgt cttcggtttc gttatataac agttgggctt
     2341 ctggtgcacc acgacgcact tcagagagag ccgtaatgac cacggtttta tcttcaaagc
     2401 cttgtttaat ggttaactcc atacctattt ttgcttcttt actgacttta actcgttgcc
     2461 cttcacaatg aaccttgccg ccttcaatgg catcttttgc taacgaacgt gttttaaaaa
     2521 aacgtgcagc ccata
//
LOCUS       DAOQMB010000341         2533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25104_length_2533_cov_14.086360, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000341 DAOQMB010000000
VERSION     DAOQMB010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25104_length_2533_cov_14.086360"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="PLY05_09690"
     CDS             <1..1317
                     /locus_tag="PLY05_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HNC03609.1"
                     /translation="IFQTVKHKVLQTKPFDEFIEQPIPEVATLKLEEIDVSNPFLYKQ
                     KKWQSYFKRLRDEAPVHYQKHSPFGAFWSVTRYEDIVFVDKNHDIFSAEPAIIIGQTP
                     EGLPAEMFIAMDPPKHDKQRQAVQDVVAPKNLKELEGLIRTRTQQVLDELPVNTPFDW
                     VQNVSIELTTRMLATLFDFPYEQRHKLVYWSDIIAGSPQATGGPGSSNDEFYNAAYEM
                     TKQFSELWYQKAADKAAGKPMGYDVMSMLVYSKDTEDLIKKPMEFMGNLALLIVGGND
                     TTRNSMSGGVYALNLFPNEFTKLKNNPSVIPNMVSEIIRWQTPLAYMRRIAKQDVELN
                     GQLIKKGDKVVMWYVSGNRDERAIDNPDAFIIDRKGARNHLSFGFGVHRCMGNRLAEL
                     QLRILWEELLKRFDNIEVLAEPEIVQSNFVRGYAKMMVKVTPKKSV"
     gene            1386..>2533
                     /locus_tag="PLY05_09695"
     CDS             1386..>2533
                     /locus_tag="PLY05_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC03610.1"
                     /translation="MSNQVCLIIGASHAAAQLATSLRQEGWQGDIIMIGDEAYLPYHR
                     PPLSKTFLAGDKEIDDLLIRPASFYDKSAIQFKQGRVTYIDRQQQKITLNDGTQLNYD
                     KLALCLGARVRTAGLTGENLAGVHYLRNIADIQAIKPYVVQGKRAVIIGGGYIGLETA
                     ASLKKLGMDVVVLEMAERVLQRVTAPEMSEFYTRIHQQQGVAIYTKMTVSEIVGEEQV
                     RAVKCADGREFLADLVIIGIGVVPNVELAEQADLEVNNGVVVDEFCQTNDPNIVAAGD
                     CANHFNAIYGRRMRLESVPNASDQAKSAAAAICGVHKPYKSLPWFWSDQYDLKLQIAG
                     LSQGYDQVVIRGDSQHSRSFAAFYLQQGKLIAADCINRPQEFMLSKKI"
BASE COUNT          743 a          445 c          600 g          745 t
ORIGIN      
        1 atttttcaaa cggtgaaaca taaagtatta caaactaagc cctttgatga gtttattgag
       61 cagccgattc ctgaagtagc gacacttaaa ttagaagaaa ttgatgtcag taatccattt
      121 ttatataagc aaaaaaaatg gcagtcttat tttaaacgcc tgcgtgatga agcacctgtt
      181 cattatcaaa aacatagtcc atttggtgca ttttggtcgg tgacacgtta tgaagatatt
      241 gtttttgttg ataaaaatca cgacatattt tctgctgaac ctgcaattat tattggtcaa
      301 acgcccgaag gtttacctgc tgaaatgttt attgcgatgg atccacctaa gcatgataaa
      361 cagcgtcaag ccgtacaaga tgtggttgct cccaaaaatt taaaagaact agagggttta
      421 attcgcacac gcacccaaca ggtattagat gaattgcctg tgaatacccc ttttgactgg
      481 gtacaaaatg tgtcgattga attaaccaca cgtatgttgg cgactttgtt tgattttcct
      541 tatgaacaac gtcataaatt ggtttattgg tcagatatta ttgcaggtag cccacaagca
      601 acaggcgggc cgggtagtag taatgatgag ttttacaatg ccgcttatga gatgaccaaa
      661 caattttctg agctttggta tcaaaaagcg gcagataaag ctgctggtaa acccatgggt
      721 tatgatgtga tgagtatgtt ggtgtattcc aaagacaccg aagacctcat taaaaagcca
      781 atggagttta tgggcaattt agccttgtta attgtcggtg gtaatgacac cacacgtaat
      841 tcgatgtcgg gtggggtgta tgctttaaat ttattcccga atgagtttac caagctcaaa
      901 aataatccga gtgtgattcc aaacatggtc tcggagatta ttcgttggca aacgccctta
      961 gcttatatgc gtcgtattgc taagcaagat gtggagttaa atggccagtt aattaaaaag
     1021 ggcgataagg tcgtcatgtg gtatgtgtca gggaatcgag atgagcgcgc gattgacaat
     1081 cccgatgcct ttattattga tcgtaaaggt gcacgtaacc atttatcgtt tggttttggt
     1141 gtccatcgtt gtatgggtaa tcgtttagcc gaattgcaat tgcgtatttt gtgggaagag
     1201 ttattaaaac gttttgacaa tattgaagtg ttggctgagc cagaaatagt acagtctaac
     1261 tttgttagag gttatgccaa aatgatggta aaagtcacgc ctaaaaagtc cgtttaaaat
     1321 cgtttgaatg accatcaccc caaccctctc ccaaaaggag agggaactat tgcaggatta
     1381 acaccatgtc aaatcaagtt tgtctgatta ttggtgccag ccatgcggct gcacagttag
     1441 ccaccagttt gcgtcaggaa ggttggcaag gcgacattat catgatcggc gatgaagctt
     1501 atttgccata ccatcgtcca cctttatcaa aaactttttt agcaggtgat aaagaaatag
     1561 atgatttgtt gattcgccca gcgagttttt atgacaaaag tgccattcag tttaaacaag
     1621 ggcgagtgac atacattgat cgtcaacaac agaaaatcac tcttaacgat ggcacacagc
     1681 ttaactacga taagctagcc ttgtgtttag gtgcaagagt acgcaccgca gggctaacag
     1741 gcgaaaattt agctggtgtg cattatttgc gtaatattgc ggatattcag gcaattaaac
     1801 cgtatgttgt acaaggcaaa cgtgcagtga ttattggtgg cggttatatt ggcctagaaa
     1861 cagcggcttc cttaaaaaaa ttaggtatgg atgtggtggt gctagaaatg gcggagcgag
     1921 ttttacaacg cgtcactgcg cccgaaatgt ctgagtttta tacccgtatt catcaacaac
     1981 aaggcgtagc catctatacc aaaatgactg tgagtgaaat tgttggtgaa gaacaagtgc
     2041 gtgcagtgaa atgtgcggat ggacgagagt ttttggcaga cttggtgatt attggtattg
     2101 gggttgtgcc taatgttgag ttggccgagc aagcagattt agaggtcaat aatggggttg
     2161 tggtcgatga attttgccaa accaatgatc cgaatattgt ggcagcaggc gattgtgcca
     2221 atcattttaa tgccatttat ggccgtagaa tgcgtttaga gtctgtgcct aatgccagcg
     2281 accaagccaa atcagcggca gcagccattt gtggtgtgca taagccttat aaaagtttgc
     2341 cgtggttttg gtcagatcaa tatgatttga agttacaaat tgctggttta agccaaggtt
     2401 atgaccaagt ggtgattcgt ggtgatagtc aacatagtcg cagttttgcc gcattttatt
     2461 tgcagcaggg caaattgatt gctgcggatt gtattaaccg cccacaagaa tttatgctca
     2521 gtaaaaaaat tat
//
LOCUS       DAOQMB010000342         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25509_length_2512_cov_4.030525, whole genome shotgun sequence.
ACCESSION   DAOQMB010000342 DAOQMB010000000
VERSION     DAOQMB010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25509_length_2512_cov_4.030525"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLY05_09700"
     CDS             complement(<1..695)
                     /locus_tag="PLY05_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC03611.1"
                     /translation="MANKTRQRKKAADLGTTSEATTALPTTIESSLPTTTPLVLAETN
                     QFVAIDSSAITTAEQSENTTNSAVTLTEDTPLVVPTVIEQFNNWCLANHQWSVMAEAV
                     VGLTHRDKNLPCQDAVACQTVPRTCLVVCDGAGSSVLSELGANALCQGLTLLCHSLEA
                     LWHDLLDNEATNDSLLNTLTRIVLRHAKGIMQNLATTHKREVRDFRSTLLMLVVGKAH
                     LFWLKVGDGALVI"
     gene            complement(746..1858)
                     /locus_tag="PLY05_09705"
     CDS             complement(746..1858)
                     /locus_tag="PLY05_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03612.1"
                     /translation="MYLDVIQLMNVFTNNLKTHKANTSPSTAQSNTAATSTTQQAQTM
                     TAITVYDANHSAKKLGVVLAKGGEGSVYPLQERSEVLVKLYHPEVLKKLQPALQQKIG
                     AMLQHKPQKTDLLSWPVLDVFNQQGDWVGYGMRRKQGLKLQFLAHALLYKKHFPHFNR
                     RDIVQVLLSLIEQVQQLHRQKIMIGDYNLNNFLCDPHSKTISLIDCDSYQCQFNGQFF
                     PCPVGSPDLTAPEHQNKSFKDVVRNPQSEVFSLAIIMFKCLMLDRHPYDMVNGEDPVS
                     NMNSGRFAYGVGNQGIPKGDWYNIWSHMPFQLKSLFITTFSEGALNPQVRPSLDKWHE
                     VLSRYVKEMDKGWHEVAIKPAAAKTRDYRGSNSMSS"
     gene            complement(1943..>2512)
                     /locus_tag="PLY05_09710"
     CDS             complement(1943..>2512)
                     /locus_tag="PLY05_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03613.1"
                     /translation="GNAINQLNQGFQHFINAVQQDEVAACSVEVGVITAGGVVREQLA
                     FTSAISLESVQAFGASGNTPLGEAVNLALNKLEQRKKEYQRNGVAYYQPWLIIISDGA
                     PTDSYQHAAAKSRQLSQERKLVCLPIGVEGANTAVLSEFSSRGAKALSGLKFHEFFEW
                     LSASMSRVSASASTSSAVNLPPTNSWEAI"
BASE COUNT          699 a          568 c          519 g          726 t
ORIGIN      
        1 tcaatcacca acgcaccatc ccccactttt aaccaaaaca aatgcgcttt gcccaccacc
       61 aacattaata aggtagagcg aaaatcgcgc acctcacgtt tgtgagtcgt tgccaagttt
      121 tgcataatgc ctttggcatg gcgcagcaca atgcgagtga gcgtatttaa taagctgtca
      181 tttgttgcct cgttatccaa caagtcgtgc cataatgcct ctaagctatg acataacaac
      241 gttaaacctt ggcacagggc atttgcgcct aattccgata acaccgagct acctgcacca
      301 tcacacacca ccaaacaagt gcgtggcacg gtttgacaag ctaccgcatc ttgacagggt
      361 aaatttttgt cacgatgcgt taaaccaacc acggcttcgg ccatcaccga ccattgatga
      421 ttggccaaac accagttgtt aaattgctca ataacggtag gaacaactag aggagtgtct
      481 tctgtgaggg tgacagcact attggtggta ttttcgcttt gctcggctgt ggtgattgca
      541 gaactgtcta tggccacaaa ttgattggtt tctgctaaca caagcggtgt tgtggtaggc
      601 aggcttgatt caatagttgt tggtaacgct gttgttgctt cacttgttgt gcccaagtcg
      661 gctgcttttt tgcgttgacg cgttttgtta gccatgattt gtttccacct taatataaat
      721 tgacacataa acatcagtac tgagcttagc tactcatgga gtttgaacca cgataatctc
      781 tggttttggc agcagcaggt ttaatagcca cttcgtgcca acctttgtcc atttctttaa
      841 catatcttga taacacttcg tgccatttgt ctaaactggg tcggacttgt ggattaagcg
      901 caccttcgct aaaggtagta ataaacaagc ttttaagttg aaagggcata tggctccaaa
      961 tgttatacca gtcgcctttg ggaataccct gattgcctac accgtaagca aaacgacccg
     1021 aattcatatt gctaacgggg tcttcaccat taaccatatc gtaagggtgg cgatccaaca
     1081 tcaaacactt aaacataata attgccaaag aaaacacttc cgattgagga ttgcgtacta
     1141 catccttaaa tgatttattt tgatgttcgg gagcagtaag atctggacta cccacaggac
     1201 aaggaaaaaa ttgaccatta aattggcatt gataactatc gcaatcaatc aaactaatgg
     1261 ttttgctgtg tgggtcgcac aaaaaattat tgaggttgta atcgccaatc ataatttttt
     1321 gacgatgaag ttgttgaact tgctcaatta aacttaacag tacttgcaca atgtcacggc
     1381 gattaaagtg aggaaaatgt tttttatata acaaagcatg tgccaaaaac tgcaacttta
     1441 aaccttgttt gcggcgcatt ccataaccca cccaatcgcc ttgttggtta aaaacatcaa
     1501 gcacaggcca actcagtaaa tcagtttttt ggggtttgtg ctgcaacatt gcgccaattt
     1561 tttgttgtaa agcaggttga agttttttta agacttcggg gtgatataac ttaactaata
     1621 cctcgctacg ctcttgtaaa ggatatacac tcccttcacc acctttagct aaaacgacac
     1681 ctaacttttt ggcagagtga ttagcgtcat aaacggttat tgcagtcatc gtttgcgcct
     1741 gttgtgtggt tgaagtagca gccgtatttg actgagcggt agatgggcta gtgttagctt
     1801 tatgggtttt aagattatta gtgaagacgt tcattagctg gattacatct aaatacatga
     1861 cttgctcccc gttttggata aacaccttaa ctgcttgaca tggtcatcat tgcccccaca
     1921 acagggggca tggctaaatg acttaaattg cctcccaact attagtagga ggaagattta
     1981 ctgccgaact ggttgatgcg gatgcagaaa cacgactcat gctggccgat aaccattcaa
     2041 aaaactcatg aaacttaaga ccgcttaatg ctttggcacc gcgtgacgaa aactcactca
     2101 ataccgccgt atttgcacct tctacaccaa tcggcaaaca tactaatttg cgctcttgag
     2161 acaactgacg gcttttggcg gctgcgtgtt gataactgtc ggtaggcgca ccatcgctga
     2221 taataattaa ccacggttgg taataagcaa caccattacg ctgatactct tttttgcgtt
     2281 gttctaattt gtttaaggcc aaattaaccg cttcgcctaa tggggtatta ccgctcgcac
     2341 caaaagcctg taccgattcc aacgagatgg cactggtaaa ggctaattgc tctctcacca
     2401 caccgcctgc ggtaatcacg cccacttcta ccgagcaagc cgctacttcg tcttgttgaa
     2461 ctgcattaat aaaatgttga aagccttgat tgagctgatt gatggcattg cc
//
LOCUS       DAOQMB010000343         2510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25544_length_2510_cov_10.000407, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000343 DAOQMB010000000
VERSION     DAOQMB010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25544_length_2510_cov_10.000407"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1031)
                     /locus_tag="PLY05_09715"
     CDS             complement(117..1031)
                     /locus_tag="PLY05_09715"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HNC03614.1"
                     /translation="MNDDLDPKLQHLYRQIPKEQPSQAVDSAILQAAQQSIANKTYWR
                     PVWGIAASVVMMSSMVWYWQAQQPQELARAVAVSASLPKPMMESVAKPVEQDEQAVNL
                     LSTDNLSAAPTIEQKPAKKSESLAKTKAQNPPQVINELKELAAAPAPVMSVEKPQQLN
                     KQMMADKNIVAKEEIESPLAASTVAKLEQTSSSLRVKQSLNKTTESHNQQAIERIRRV
                     FEKNFVELNSVYQQALKEDANLEQGVVHFKLIIDEKGTVASCMVASSTLNNPELEAKF
                     VEIVKKFNFGEATEEWKGTYPVYFDNTQ"
     gene            complement(1028..1597)
                     /locus_tag="PLY05_09720"
     CDS             complement(1028..1597)
                     /locus_tag="PLY05_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC03615.1"
                     /translation="MITKQDGQQSDEQLMLAYQAGQQKAFDELYQRYRQPIFAYLMRN
                     IGSQMQAEEVYQEVWLSLIKQRTRYTATSSFRSYLFCIAHSRLLDFYRQQGKLAANDQ
                     LHEDDATLIACAWHEPDMQLEEQRLIAKLQWCISQIPEEQRAVLVLKLESELSLQTIA
                     EQLNIAFETLKSRFRYAQAKLQRCLGEES"
     gene            complement(1672..>2510)
                     /locus_tag="PLY05_09725"
     CDS             complement(1672..>2510)
                     /locus_tag="PLY05_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3520 domain-containing protein"
                     /protein_id="HNC03616.1"
                     /translation="KQGINRILLATDGDFNVGVTSHEQIKEMVKRERETGVTLSTLGF
                     GQGNFNDAMMEQIADVGNGNYSYIDSVSEARKVLVDELSSTFNTVAKDVKVQIEFNPN
                     WVSEYRLIGYENRQLNREDFNNDKVDAGDVGAGKSVTAIYEITPKGAASSVDPLRYQN
                     EKQASTTKPTPEFAFLKIRYKQPHATSSTLLTQPLMLPKSITPFTSATNDHRFATAVA
                     GFGQLLRQSSNMGNLSYGQVLQIAQQAKGIDKMGTRAEFIQLVQNVQALSQKPEAQAQ
                     AE"
BASE COUNT          685 a          545 c          435 g          845 t
ORIGIN      
        1 gtgcatggga aatcgccgca tttgctttta aagatgaacc aagccctgcg gaattagcag
       61 aagtcggtat tgttattgag taatcattaa atgctcaaac tttttgagca tttattttat
      121 tgtgtattat caaaatacac aggatatgta cctttccact cttctgttgc ctcaccaaaa
      181 ttaaactttt ttactatctc aacaaatttt gcttctaact caggattatt taaggtgcta
      241 gaagcaacca tacaacttgc tactgtgcct ttttcatcaa taatcagttt aaaatgtacc
      301 actccttgtt ctagattcgc atcttctttt aatgcttgtt gataaacgct attaagctca
      361 acaaaattct tttcaaatac tcgtctaatg cgctctatag cttgttgatt atgagactca
      421 gtagttttat ttagactttg tttcacacgc aatgaggatg atgtttgttc taatttagcc
      481 accgtacttg ccgccaaagg tgattctatt tcttctttag ccactatgtt tttatcagcc
      541 atcatttgct tatttaactg ttgaggtttt tctacagaca tcacaggtgc aggagcagca
      601 gctaactctt ttaactcatt gataacttga ggaggatttt gtgctttggt tttggctaat
      661 gattctgatt ttttggctgg tttttgctct atagtcggtg cagcacttag attgtctgta
      721 gataataaat ttacagcttg ttcatcttgt tctactggtt tggcaacaga ctccatcatg
      781 ggttttggta atgaagcgga taccgccacc gcacgtgcta actcctgtgg ttgttgcgcc
      841 tgccaatacc aaaccatact actcatcatc accacacttg ctgcaatgcc ccacacaggc
      901 cgccaataag ttttgttcgc tattgattgt tgtgctgcct gtaaaatagc agagtcaact
      961 gcttggctag gctgctcttt aggaatctgc cgatataaat gctgtaattt agggtctaaa
     1021 tcatcattca cgattcttct cctaaacaac gttgtaattt tgcttgtgca taacgaaaac
     1081 gactttttaa ggtctcaaag gcaatattga gctgctcagc aatggtctgt aagcttaatt
     1141 cagactctaa ttttaggact aaaactgctc tttgctcttc tggaatttgg ctaatacacc
     1201 attgcaattt cgcgattaaa cgctgctctt cgagttgcat atcaggctca tgccacgcac
     1261 aagcaattaa cgtagcatca tcttcatgca gttggtcatt agccgctaac ttaccctgct
     1321 gccgataaaa atctaataaa cgactatgcg caatacaaaa caaataactt ctaaaactgg
     1381 atgttgctgt gtagcgtgtg cgttgtttaa ttaaacttaa ccacacctct tgataaactt
     1441 cttcggcttg catttgcgag ccaatattac gcattaaata ggcaaaaata ggctgtcgat
     1501 aacgttgata caattcatca aaggcttttt gttgtcctgc ctgataggca agcattaatt
     1561 gctcatcaga ctgctgtcca tcttgcttag ttatcattat ctatccgttt tagactctta
     1621 ttgattaaat ttaccaagtt ttagctaaaa cggatagatc atttaaacaa actactcagc
     1681 ttgagcttgc gcttcaggtt tttgacttaa tgcctgaaca ttctgcacca actgaataaa
     1741 ctctgctcgt gtccccattt tatcaatccc tttagcttgc tgtgctattt gtaaaacttg
     1801 cccataactc aaattgccca tattgcttga ttgacgcaat aactgaccaa agcctgcaac
     1861 ggcggtggca aaacgatgat catttgtcgc acttgtaaag ggggtaatac ttttgggcaa
     1921 cattaatggc tgagttaaca aagtgctact tgttgcatga ggttgtttgt agcgaatctt
     1981 taaaaaagca aactcaggcg ttggtttagt ggtagatgct tgtttttcgt tttgataacg
     2041 taacggatca accgatgatg ctgcaccttt aggagtgatt tcataaatgg ctgtcacgct
     2101 cttacctgcc cccacatcgc ctgcatcgac tttatcatta ttaaaatctt cgcggtttaa
     2161 ttgacgattt tcgtaaccaa ttaaacgata ttcactcacc caattaggat taaattcaat
     2221 ttgtacctta acgtctttag caacggtatt aaacgtgcta cttaattcat ccaccaatac
     2281 tttacgcgcc tcactcaccg agtcgatata actgtaatta ccattaccca catcggctat
     2341 ttgctccatc atcgcatcat taaagttacc ttgaccaaaa cctaaggttg ataacgtcac
     2401 tcctgtttca cgctcacgtt tgaccatctc tttaatttgc tcgtgactgg tcacacccac
     2461 attaaaatca ccatcggttg ccaataaaat acggttaata ccctgtttaa
//
LOCUS       DAOQMB010000344         2509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_25575_length_2509_cov_3.559902, whole genome shotgun sequence.
ACCESSION   DAOQMB010000344 DAOQMB010000000
VERSION     DAOQMB010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25575_length_2509_cov_3.559902"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..383
                     /locus_tag="PLY05_09730"
     CDS             126..383
                     /locus_tag="PLY05_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03617.1"
                     /translation="MNQHAVNLFTMQLVFNRLLKLASQKMQVLIVDYYTIATSLNITN
                     NQFILSIKLLSKSDYIEHIFDGDNLYFKITNIGMEYANEQV"
     gene            370..1278
                     /locus_tag="PLY05_09735"
     CDS             370..1278
                     /locus_tag="PLY05_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03618.1"
                     /translation="MSKYRKIDVRIWNDAKFREFSHNAKLVFFLLLTHPNMTSIGAMR
                     ATLSGLAEEMNMDLEAFREAFREAFRKGMVKHDERACLIALPNFIKYNQPESPNVVKG
                     WVNALDLLPECDLKNEVISISANALEGYGKAFQEALPKAFREAFRKGMPNQEQEQEQE
                     QDNNNNTHEDEYIKNATKDTRKTFAIDFNWRPDKTMFAAYCQRKLVKPSQLSEDLFNK
                     FVDKVSAKGECKTEAQWCENLASYLKTCLSNPPKPTQQQKTDYTNMPPPLEPIRPMPK
                     VKLTPEQRAEQDRLMAELIQKTKMGL"
     gene            1281..>2509
                     /locus_tag="PLY05_09740"
     CDS             1281..>2509
                     /locus_tag="PLY05_09740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC03619.1"
                     /translation="MFEEEKIPFSESLEQTILGSMFTLDLYSIVAEIVTEDDFYFESH
                     RIFVRTIRGMVARGVVIDMAMVIDELKRLNVYESLGGDNRVMEFLKGLYASSEKNLIQ
                     YCERLKELSLERKMLGAAQKITHMILHKDGELTTRAMIETAEREILQVSNSSGIGANE
                     MQSKDSKDAIQTVINAIETARSRKDGELSGVRTGLKELDEYTDGFQKGDLIFIGARPS
                     MGKTTLGLNFAESAFLTQKLPVVIFSMESPTVQITQRLIAAHSGVNMGNIVRGEFDPY
                     QFEKVSNTIATLKNSNFIITDKGSLSPSDMRQLLRRIAREHGGIGMVLADYVQLMKLH
                     DNHKKTRNDELSEISRDLKNIAKEFNCPFLCLAQLSKDCERRPDKRPMMSDLRDCGGL
                     EQDADMIIMIYRNEVYYP"
BASE COUNT          843 a          460 c          542 g          664 t
ORIGIN      
        1 tgttttagaa ttatgctata ataaccaagc cgctacgcag gaagtcatga gcctgtggaa
       61 gtctttacct actggacgag cggcttattt cttttaagta ggtcacaatt tagtaggtgt
      121 caaacatgaa tcaacatgcc gttaatctat tcacaatgca gcttgttttt aacaggttgt
      181 taaagttagc gtctcaaaaa atgcaagtct taattgttga ctactataca atagcaacaa
      241 gcctaaatat aactaacaat caatttattt tgtccataaa actcttatca aaaagcgatt
      301 atatagaaca tattttcgat ggcgataatt tgtatttcaa aattacaaac attggaatgg
      361 agtatgcaaa tgagcaagta tagaaaaata gatgttcgta tttggaatga tgcgaagttt
      421 agagagtttt cacacaatgc taaattagtg ttttttttgc ttttaactca cccgaacatg
      481 acttcaatcg gtgcaatgag agctacatta tcaggcttgg ccgaagagat gaatatggac
      541 ttggaagcct ttcgggaagc ctttcgggaa gcctttcgaa agggtatggt gaagcatgac
      601 gaaagagctt gtttgatagc cttgcctaac ttcattaagt acaaccaacc agagtcgcca
      661 aacgtggtta aagggtgggt aaatgcgctt gatttactgc ctgaatgcga ccttaaaaac
      721 gaggtaatct ctatatctgc caatgctttg gaaggctatg gcaaagcctt tcaggaagcc
      781 ttacctaaag cctttcggga agcctttcga aagggtatgc ctaatcaaga acaagaacaa
      841 gaacaagaac aagataataa caacaacacg cacgaggacg agtacatcaa aaacgcaaca
      901 aaggacacac gcaaaacatt cgcgattgat ttcaactggc gaccagataa aactatgttt
      961 gcagcttact gccaacgtaa acttgttaaa cctagccaac taagcgagga tttatttaat
     1021 aagtttgttg acaaagtaag cgcaaaggga gagtgtaaaa cagaggcgca atggtgcgaa
     1081 aacttagcga gttatttaaa aacttgtctt agcaatccac ccaaacctac acaacaacaa
     1141 aaaaccgatt acacgaatat gccgccaccg cttgagccta ttcgaccaat gccgaaagtc
     1201 aagctgacac ctgagcagcg agccgaacaa gatagattga tggccgagct aatacaaaaa
     1261 actaaaatgg gactttaacc atgtttgaag aagaaaaaat accatttagc gaatcgctag
     1321 aacaaacaat acttggctca atgtttacgc tagatttata ttcgattgtt gccgaaattg
     1381 ttactgaaga tgatttttat tttgaatctc accgcatttt tgtgcgaact attcgcggca
     1441 tggttgcgcg tggtgttgta attgatatgg caatggtcat tgatgagcta aaacgcttaa
     1501 atgtgtatga gtcgcttggc ggtgacaatc gcgtgatgga gtttttaaaa gggctttacg
     1561 catctagcga aaaaaactta atccaatatt gtgaacgatt aaaagagtta agtcttgagc
     1621 gtaaaatgtt gggagctgct caaaagatta cacacatgat tttacacaaa gatggggagc
     1681 taacgaccag agccatgatt gaaacagcag agcgcgagat attgcaggtt agcaacagct
     1741 caggcatagg agcaaacgag atgcaaagca aagactcaaa agacgcaata cagaccgtta
     1801 ttaacgcgat agaaacggca cgaagccgaa aagatgggga attatcagga gtcaggacag
     1861 ggctaaaaga gcttgacgaa tataccgatg gattccaaaa aggcgacctg attttcatcg
     1921 gggctaggcc aagtatgggc aaaacaacac ttggtttaaa ttttgctgaa tctgcttttt
     1981 taactcaaaa attgccagta gttatttttt cgatggaatc gcctacggta caaattacac
     2041 aacgattgat tgcagcacac tcaggtgtaa acatgggcaa cattgtacgc ggtgagtttg
     2101 acccatacca atttgaaaaa gtatcaaaca ccattgccac cttaaaaaat tctaatttta
     2161 ttatcacgga caaaggcagt ttatcgccat ctgatatgcg ccaattattg cgtagaatag
     2221 cccgtgagca tggaggtatt ggcatggtgt tggctgatta tgtgcagtta atgaaactgc
     2281 acgataacca caaaaagact cgtaatgatg aattaagcga gattagccgc gacttgaaaa
     2341 acattgcaaa agagtttaat tgcccttttt tgtgcttggc tcagttgtct aaagattgtg
     2401 aaagacgacc tgacaaacgc ccgatgatgt cagacttgcg cgattgtggt ggattagagc
     2461 aagacgcgga tatgattatt atgatttatc gtaatgaggt ttattatcc
//
LOCUS       DAOQMB010000345         2484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26035_length_2484_cov_7.456155, whole genome shotgun sequence.
ACCESSION   DAOQMB010000345 DAOQMB010000000
VERSION     DAOQMB010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26035_length_2484_cov_7.456155"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..927
                     /locus_tag="PLY05_09745"
     CDS             364..927
                     /locus_tag="PLY05_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03620.1"
                     /translation="MAQFILSDDGTPLFAVITYKEFQSVAHILNEKKQEDLVTELLPV
                     KKEPVLANANDLLSKDGRFISLPHGGGYQLNIAQLVDYFLSNSISVMAINQRAQKYEI
                     YPPIQRNTLDPLIRWNCLRQTPYFNTMQSVQLFIDALVSTGLFTRTTHYYPNVFTRAV
                     NSISIVRDEALAYLKKYPISEKWTIPK"
     gene            complement(1348..2088)
                     /locus_tag="PLY05_09750"
     CDS             complement(1348..2088)
                     /locus_tag="PLY05_09750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNC03621.1"
                     /translation="MNVLSNEPVGRLWYGDIEINYQIANRERVTRRVLIKVHPDCRVE
                     VHAPESATLEAIHHAVKQRARWIAQQLAEFEAQLTYVTPRCYVSGESHYYLGKQYLLK
                     VIEDPLQPQQVKLLRGKIQVCVHQKNAEVVKGLLKEWYKKRAKEVFQRRLEVLLPQAL
                     WVVAPPVIRIQTMQKQWGSCSPEGRITLNPNLVKAKRDCVDYVILHELCHLAEHNHSE
                     HFYRLMTQVMPNWQIVKKELDDRAAVFL"
     gene            complement(2069..>2484)
                     /locus_tag="PLY05_09755"
     CDS             complement(2069..>2484)
                     /locus_tag="PLY05_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HNC03622.1"
                     /translation="HPLKQYLLFSEFEEKVKERKLDDLPSRFIGNRYPQAYFGVFKTV
                     LPDVISRLSQDENKDGLEIWIQLAFKVDELVNQAVLENSISPQSIESEIRKALLPLIF
                     KTCKDIGAGMDQAKAIVEMIVQITRVGLLHERIEQ"
BASE COUNT          755 a          491 c          429 g          809 t
ORIGIN      
        1 cctaagaaac cccgaaaaac agatcaaaaa aacaccaatt atttcctcgc ataacagcca
       61 ttatgttaag tacactgttt ttattcacat catcacaata attgttagtt agttaaattc
      121 aattctgtag gcatcttttc gagtctatcc atgaactgtt attagtagct tggtatctta
      181 cttgatctag gtattattta gattaagtaa gtaattaagt aagtttttct atatttacaa
      241 ttaagatttt tatttgatta catctatatt gtaaaaaaat tacaaccata ttgggtattg
      301 aaaaagaaga taaataggag tattgttatt actccaagca acgagtttct ataggcggtt
      361 tttatggcac aatttattct tagtgatgat gggacaccat tgtttgctgt tattacatac
      421 aaagaatttc agtcagttgc acatattttg aatgaaaaaa aacaagagga tttggttacg
      481 gaattgctcc ctgttaagaa agagcctgta ttggcgaacg cgaatgattt gctatcaaaa
      541 gatggacgtt ttattagtct tcctcatggt ggtggctatc agttaaatat tgctcagctt
      601 gttgactatt ttttgagcaa ctctatttct gtgatggcta ttaatcaacg agcacagaag
      661 tatgagattt atccaccaat ccaacgtaat acgcttgatc cattgatacg ttggaattgc
      721 ttaagacaaa caccctattt caatactatg caatctgtgc agttatttat tgatgcttta
      781 gtttcaactg gattatttac acgtacaaca cattactatc cgaacgtgtt cacacgagct
      841 gtaaattcaa tatctattgt aagagatgag gcattagcgt acctcaagaa ataccctata
      901 tcagaaaagt ggactatccc taagtaatag gtcagctatt aaaagtggtg gattactcca
      961 ccacataaca ccttgcctta aaatttaaag gaaagtgctt ggttgttact tatagagtga
     1021 agccattgat tagcgatgtt tatagcagct gttacactgt caatcactga aagctcccta
     1081 cattggctgt taacctcagc aacaaatact atttgaccac attttgtttc aaggattctt
     1141 gcttcatata agcatgaatt gcttgaatca tcacacttgg ccattttgat agtataacta
     1201 tcatgtaaac taaatttgtg tcgcatttta ttgctccaat atattttcaa agaaacaagc
     1261 cactatggct tatccctatt ttaattcgca aactgggaaa gagtgggtga gtaagagagt
     1321 tttataaacg ctatcaaata gctcagctca caaaaacaca gccgccctat catcaagctc
     1381 ttttttaaca atctgccaat taggcatcac ttgagtcatt aaccgataaa aatgctcgct
     1441 atggttatgt tcagcaagat gacacagttc atggagaata acgtagtcaa cacaatcacg
     1501 ttttgctttg actaaattag gattgagcgt gattctcccc tctggtgaac aactacccca
     1561 ctgtttttgc atcgtttgta ttctgattac aggaggtgct acaacccaca atgcttgtgg
     1621 caataagact tctaaacggc gttgaaaaac ctctttggca cgttttttat accactcttt
     1681 taatagtccc ttaacaacct cagcattctt ttgatgaaca caaacttgta ttttccctcg
     1741 taacagtttg acctgctgag gttgtaatgg gtcttctatc acttttaaaa gatactgttt
     1801 gcctaaataa tagtgactct cgccactgac ataacaacga ggggtgacat aggttaattg
     1861 ggcttcaaac tccgccaact gctgagcaat ccaacgcgct ctttgtttta cggcatggtg
     1921 tattgcttca agcgttgcgc tttctggcgc gtgaacctcg acacgacaat caggatgaac
     1981 cttaatgaga actcgtcgtg tgacacgctc tcggttggcg atttgataat taatttcaat
     2041 atcgccatac cataaacggc ctacaggctc attgctcaat acgttcatgc aataacccaa
     2101 ctcgtgtaat ttggacaatc atttcgacaa tcgctttggc ctgatccatc cctgcaccaa
     2161 tatctttgca ggttttaaaa ataagcggca ataacgcttt tcgtatttct gattcaatgc
     2221 tttgtgggct gattgagttt tctaatactg cttgatttac caactcatca actttaaaag
     2281 ccaactgaat ccatatttct aaaccatctt tattttcatc ttgggataaa cggctgatta
     2341 catcaggcaa cactgttttg aatacgccaa aataggcttg agggtaacga ttgccgataa
     2401 atcgactcgg taaatcatcc agtttccgct cttttacctt ttcttcaaac tcagaaaaca
     2461 acaaatactg tttaagtgga tggt
//
LOCUS       DAOQMB010000346         2478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26142_length_2478_cov_17.647544, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000346 DAOQMB010000000
VERSION     DAOQMB010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26142_length_2478_cov_17.647544"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLY05_09760"
     CDS             complement(<1..458)
                     /locus_tag="PLY05_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03623.1"
                     /translation="MTFKKILLIDDDVVLQAIAQDHLTRDGFNVVCASDAATGIQAVI
                     DEKPDLVLLDVVLPDSDGYKVCQELRALPISVHLPIIMLTGLDDSHSIVQAYDYGATD
                     FFVKPINWKLLGYRIHYVLRANRTLEQLAHSEAALANAQRLAHLGSWEWYP"
     gene            complement(588..968)
                     /locus_tag="PLY05_09765"
     CDS             complement(588..968)
                     /locus_tag="PLY05_09765"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HNC03624.1"
                     /translation="MHYQKNTTKIDEATLTSLGGASGLASPLVRRVITLYRQEAEKMV
                     HGIEKALSGGDVATAFRAAHSLKSSSASIGAHTLSEVAKQIEALLKQGQLQQAQPWLV
                     VLRTEYTQLMDELVFFEQKISAIE"
     gene            complement(1260..2033)
                     /gene="rph"
                     /locus_tag="PLY05_09770"
     CDS             complement(1260..2033)
                     /gene="rph"
                     /locus_tag="PLY05_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HNC03625.1"
                     /translation="MANLQQALAASITKDTVFRRGNGRNPDELRAIKITRNYTKHAEG
                     SVLIEFGDTKVLCTASVEVGVPRFLKGKGEGWITAEYGMLPRATHTRNDREASKGKQG
                     GRTLEIQRLIGRSLRAMVDLKKLGEHTITIDCDVIQADGGTRTASITGGAVALVDALN
                     HMRAKKMIKHDPLTQLIAAISVGVVKGQAVLDLDYDEDSNCDTDMNVVMSQLGGFVEV
                     QGTAEGKPFSREQADAMLILAEQGIKTLIEKQQDALKQS"
     gene            2210..>2478
                     /locus_tag="PLY05_09775"
     CDS             2210..>2478
                     /locus_tag="PLY05_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03626.1"
                     /translation="MIYSMTAFARRELRGDFGLLVCEIRSVNQRYLEANLRLSDVVRE
                     LEMPLRDKLRQQLSRGKVDVWIKHEAIEGNVSNIHINQDLAARLVA"
BASE COUNT          756 a          512 c          438 g          772 t
ORIGIN      
        1 ggataccact cccaactccc taagtgtgct aaacgttgtg catttgctaa agcagcttca
       61 ctatgagcaa gttgttctaa agtacgattg gcacgtaaca cataatgaat acgatagccc
      121 aacaacttcc agttaatggg ttttacaaaa aagtcagttg caccataatc ataagcttga
      181 acaatagagt gactatcatc taagcccgtc agcataatga ttggcaaatg tacagaaatc
      241 ggtaaagcac gtaattcttg acagacctta tagccatcag agtctggtaa aaccacatct
      301 aataatacaa gatcaggttt ttcatcaata actgcttgaa tacctgttgc cgcatcactg
      361 gcacacacga cgttaaagcc atcacgtgtt aaatgatctt gagcaatcgc ttgtaatact
      421 acatcgtcgt caattaataa aatcttttta aaagtcatga tgatactcaa attgtatcaa
      481 atcgcagata gaatgcttgg aatgtacaca cttcacataa gtttgttaat agccatgact
      541 aaatctatag caaaaacaaa cttaactgtg caaatctcaa actaaaacta ttctatcgcc
      601 gatatttttt gttcaaagaa taccagctca tccatcaact gagtgtactc agtacgcaat
      661 acaacaagcc aaggttgtgc ttgctgcaat tgaccttgtt tgagaagtgc ctcaatttgt
      721 ttagccactt cagacaaagt atgcgcacct atggaagctg acgaagattt gaggctatga
      781 gctgcccgaa aagcagtagc aacatctcca ccagacaagg ctttttcaat cccatgaacc
      841 attttttcgg cttcttggcg atacaaagta atcacccgtc taaccaatgg tgacgctaac
      901 cccgacgcac cccctaaact tgttagcgta gcttcatcta tttttgttgt atttttttga
      961 taatgcattt aattttttat tccctaatgt gtcgcactat gctgccaact gataaagttg
     1021 tttaacatct ttatgctttt gactaaattg acatatttgc tacacaattc acaataatta
     1081 tagcgaattc gctaggcaaa agtatttata ccgcaaattt atatcagttt tatgatgagc
     1141 atctcatttt tagcacaaat attatgctat tggatcagct tagcattata taaaaacgct
     1201 agaaaccata aaaaagggtt tagaatgata tttgtacaat tctaaaccca tcctcgcaat
     1261 taggattgct ttaaagcgtc ttgttgtttt tcaattaaag ttttaattcc ttgttcagct
     1321 aaaattaaca tcgcatctgc ttgttcacgg ctaaacggtt taccttcggc tgtgccttgt
     1381 acttccacaa aaccacctaa ttggctcatg acgacattca tatcggtatc gcaattagag
     1441 tcttcgtcat aatctaaatc tagcactgct tgtcctttaa ccactcctac cgaaatggcg
     1501 gcaattaatt gggttaatgg gtcatgttta atcatttttt tagcacgcat atggtttaag
     1561 gcatcgacta aagccactgc cccacctgta atcgatgctg tgcgtgtgcc accatcagct
     1621 tgtatcacat cacagtcaat cgtaattgta tgttctccta atttttttaa atcaaccata
     1681 gcgcgtaaac tacggccaat taaacgttgg atttctaaag tacgaccacc ttgtttgcct
     1741 tttgatgctt cgcggtcatt acgggtatga gtagcacgag gcaacatgcc atattcagca
     1801 gtaatccaac cttcgccttt gccctttaaa aagcgtggca cacctacttc gacagaagca
     1861 gtacacaata ctttagtatc accaaactca attaacactg agccttcagc atgtttagta
     1921 taattacggg taattttgat ggcgcgtaat tcatcagggt tacgtccatt accacgtcta
     1981 aatacagtat ctttagtaat agatgcggct aaagcttgtt gtaaattggc cattgtgata
     2041 atcctctatt tataaatcga ttaggctctg aaatttttag tcaaaatttg caggctttaa
     2101 aacgcactga ttgcagcact atgcctaatc tatgacaaaa ttttgcgccg attatacgat
     2161 gaagccgtta caatcggcgg ttaagaataa aattttttga ggtttattga tgatttacag
     2221 tatgacggct tttgcccgcc gcgaattaag aggcgatttt ggattattag tgtgtgaaat
     2281 tcgctctgta aatcaacgtt atttagaagc gaatttacgc ttgtcggatg tggtgcgtga
     2341 gttagaaatg cccttacgcg ataaacttcg tcaacaatta agccgtggta aagtcgatgt
     2401 gtggattaaa catgaagcga ttgaaggcaa tgtctctaat attcatatta atcaagattt
     2461 agcagcacgt ttagtggc
//
LOCUS       DAOQMB010000347         2471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26279_length_2471_cov_16.940811, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000347 DAOQMB010000000
VERSION     DAOQMB010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26279_length_2471_cov_16.940811"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..2400
                     /locus_tag="PLY05_09780"
     CDS             196..2400
                     /locus_tag="PLY05_09780"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNC03627.1"
                     /translation="MSRVYLQVALPVPLAQVFDYSIPASFSHTPHIGARIKVPFGKRE
                     LIGVIIGVTETPESPLDKIKPARQLIDEHSLFPPQLMQLLTWAAQYYHYPIGEVFSTA
                     LPTLLRQGEDLYDNPIHWRLTPVGLQITADTLSRSAKQQAAWQALEPHQSHGISEDFL
                     KKVQIERATLQALAKKGFAEEFGQLAIQTGTPQLKYAESPLVANESQTTAIHAITEHL
                     GQFKSFLLDGVTGSGKTEVYLQSIASCLAQHKQALILVPEIGLTPQTVARFRSRFAVE
                     IVMLHSGLTDRERLRAWRRAYLGHAAIVIGTRSTLFTPMPHLGLIIVDEEHDLSFKQQ
                     DNFRYNARDLAIFRAQLASCPVVLGSATPSLESLANAKQGRYQHLFLKERAANVSQPT
                     IKLIDLRGQKLQAGLSPALKEAISLRLKKDEQVIIFLNRRGFAPVLLCHDCGWQAKCP
                     RCDAKITLHRNPLRLHCHHCGFSQQPPNFCPECNSKELKAIGAGTVRVEEALQELFPK
                     VPIHRIDRDSTQRKDSWHNLYADIENNKAAILVGTQMLAKGHHFPNVTLVALLEADSG
                     LFGADFRASERVAQLITQVAGRAGRAHKAGLVLLQSHQPEHPLLQQLLSTGYQGFAEL
                     ALAEREMIGMPPYGYLALVRAEAHYPEMALNFLTEAMSRGQVLDNAPEFWGPIPAPME
                     KKAGIFRAHLLLKSSQRRRLQQFLHRWLTIVNDLPEAKRLRWHIDVDPQELA"
BASE COUNT          702 a          520 c          524 g          725 t
ORIGIN      
        1 ggtaattgca gccagcgact ccaaaaaata aatcgagctg ctgcgtgtgt aaactgttga
       61 ctcataaaaa ctcctcatta aaccaaagat gatactcttg tctttacaca ccaaaaataa
      121 gtcggcatag tttagggctt attacacttt ttttgtatta gcttgttata caagtttcag
      181 gattttcatt ttttaatgtc acgcgtttat ttacaagtcg ctttacctgt gcctttagca
      241 caagtgtttg actacagcat tcctgcgagt tttagtcata cgccacatat tggcgcgcgg
      301 attaaagtac cttttggcaa acgtgagtta attggcgtga ttattggtgt gaccgaaaca
      361 ccagaatcac cacttgataa aattaaacct gcccgacagt taattgatga gcattcctta
      421 tttccccccc aactgatgca attattaacg tgggcagcac agtattatca ttaccccatt
      481 ggtgaagtat ttagcactgc tttgccgacc ctgttgcgtc aaggtgaaga cttatacgac
      541 aaccccattc attggcgttt aacacctgtt ggtttacaaa ttaccgctga cactctaagc
      601 cgctctgcca aacaacaagc cgcatggcaa gctcttgaac cccatcaaag tcatggcatt
      661 agtgaagatt ttttaaagaa agttcaaatt gaacgcgcta cgctgcaagc cttagccaaa
      721 aaaggttttg ccgaagaatt tggtcaatta gccattcaaa caggcacacc acaactcaaa
      781 tatgccgaat cacccttagt tgccaacgaa agccaaacca ccgccattca tgctattacc
      841 gaacatttag gccaatttaa aagctttttg ttagatgggg tgacaggcag tggtaaaacc
      901 gaagtgtatt tacaaagtat tgcttcatgt ttagcgcaac acaaacaagc tttaatatta
      961 gtacctgaaa ttggtttaac cccacaaact gtggctcgtt ttcgttctcg ttttgccgtt
     1021 gaaattgtca tgctgcattc aggcttaacc gatagagaac gtttgcgcgc ttggcgacgt
     1081 gcttatttag gtcatgcggc aattgtgata ggcacacgct ctaccctatt tacccccatg
     1141 ccccatttag gcttgattat tgtggatgaa gaacatgatt tatcctttaa acaacaagat
     1201 aactttaggt ataacgcgcg tgatttagcg atttttcgtg ctcagttggc aagttgtcct
     1261 gtggtgttag gttcagcgac ccctagctta gaaagcttgg ctaatgccaa acaaggccgt
     1321 tatcagcatt tatttttaaa agagcgtgcg gcaaatgtct cgcaacctac gattaaattg
     1381 attgatttac gtggtcaaaa actccaagca ggtttaagcc ctgcactaaa agaagcgatt
     1441 agccttcgac taaaaaaaga tgaacaagtg attatctttt taaatagacg aggttttgca
     1501 cctgttttgt tgtgtcatga ttgtggttgg caagcgaaat gtccgcgttg tgatgccaag
     1561 attactctac acagaaatcc cttgagattg cattgccatc attgtggctt tagtcagcag
     1621 ccacccaatt tttgtcccga atgcaacagc aaagaattaa aagccattgg tgctggcaca
     1681 gtgcgtgttg aagaagcctt acaagagtta tttcccaaag tgccgattca tcgtatagat
     1741 agagatagca ctcaacgtaa agatagttgg cacaatttat atgctgacat cgaaaataac
     1801 aaagccgcga ttttggttgg cacacaaatg ttggctaaag gtcaccactt ccctaatgtg
     1861 acgttggttg cgttattaga agccgatagt ggtttgtttg gggcagattt tagagccagt
     1921 gaacgtgttg ctcaattaat tactcaagtg gcaggtcgtg caggtcgcgc acataaagca
     1981 ggtttggtgt tgttgcaaag tcatcaacct gaacatccgt tattgcagca attattaagc
     2041 acaggttatc aaggttttgc tgagttggcg ttagctgagc gcgagatgat tggtatgcca
     2101 ccgtatggtt atttggcctt agttcgtgcc gaagcgcact atcccgaaat ggcactcaat
     2161 tttttgaccg aggcaatgag tcgaggtcag gtattggata atgcacctga attttggggg
     2221 cctattcctg cgcctatgga aaaaaaggca gggattttta gggcgcattt gctgttaaaa
     2281 agtagccaac gtcgtcgcct tcaacagttt ttgcatcgtt ggttaacgat tgtgaatgat
     2341 ttacctgaag caaaacgttt acgttggcat attgatgtag atccgcaaga gttggcttaa
     2401 gattcccaat cctctattaa caaaccttct atacgactaa actcacgaac attatgcgtc
     2461 actaaaacac a
//
LOCUS       DAOQMB010000348         2468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26337_length_2468_cov_44.138417, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000348 DAOQMB010000000
VERSION     DAOQMB010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26337_length_2468_cov_44.138417"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PLY05_09785"
     CDS             complement(<1..695)
                     /locus_tag="PLY05_09785"
                     /inference="COORDINATES: protein motif:HMM:NF023080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HNC03628.1"
                     /translation="MVTSDLIRSSLRLIGAISSSEAPAADESMDALEALNMMLNSWGA
                     VRFLSKNTPTITHTLNGSTSYTIGSGGDIDTIRPSAIFTAYYTLGGLDYPLQVLDYKT
                     YSEIGTKNIGAIPEYVVLKPDYPLSTIYVFPVGSGGVLTLSAVRPQVELNINDDVQDI
                     YPPEWIRAIKYNLAIEIAPEYGVTVSPEIVAIAKEAKDTVMRSMISIPKAKFDPLLND
                     GYTTASRSFMAGGG"
     gene            complement(737..1963)
                     /locus_tag="PLY05_09790"
     CDS             complement(737..1963)
                     /locus_tag="PLY05_09790"
                     /inference="COORDINATES: protein motif:HMM:NF023081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HNC03629.1"
                     /translation="MANTILTSQIITREAQRILHQKLNIVGNMNRQYDDRFAKRGAKI
                     GTSLDLRLPAKFTVRTGSSYSGQNVVERKVNLPVATVKGVDMAISDLEMALSLDDFSK
                     TILEPAMAQLAAQIEYDAINALYKYVPNYVGTVSTQLDYKKFQQAGQVLTENLAPSSQ
                     RCFILNPSSRVEFSDAVKGLFQSSTNIENQYKEGIVGRTGGFDVFENTMIPAHTTGTY
                     AGTPLTNGANQGSTGADNAYATTSSLITDGWSSTSLKAGDIITIADVYEVHPETKQST
                     GKLKKFTVTADISDTAGAITMTISPAIIAGGAYQNVTARAGDNKAITVLGTSATAYGQ
                     NIAFHKDAFAFVTADLEVPNGVDMAAREVYDGVSMRFVRWFDGDAGEFKSRIDILYGY
                     APVYPELAVRAVHQLA"
     gene            complement(1974..>2468)
                     /locus_tag="PLY05_09795"
     CDS             complement(1974..>2468)
                     /locus_tag="PLY05_09795"
                     /inference="COORDINATES: protein motif:HMM:NF020866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03630.1"
                     /translation="SDFDTFDDYTEALAEYKYNQKTQAQAQQYNQQAQAQKVAQEWQS
                     KVEKVRAVAPDFDEAFANVAEITFAQSTLDAVAGHEKGAEIAYLLGKDPVKAYQIASL
                     PPMQQLMAIGEIAAKTNLTRPKTVTNAPPPVKPVSGKSSIVDPNSLSMKEWVAYRNKQ
                     LRQK"
BASE COUNT          719 a          521 c          509 g          719 t
ORIGIN      
        1 ccacccccag ccataaacga gcgtgatgca gtggtataac catcattaag caatgggtca
       61 aactttgctt taggtatgct aatcatagac cgcataactg tgtctttagc ttctttggca
      121 atggctacaa tctcaggtga aacagtgaca ccatattcag gcgcaatctc aatagccaaa
      181 ttatatttaa tggcacgaat ccactcagga gggtaaatat cttgcacgtc gtcattgata
      241 tttaactcaa cctgtgggcg aactgccgat aatgttaaaa cgccaccact accgacagga
      301 aagacataaa tcgtgctaag cggataatca ggtttaagga caacatactc aggaattgcc
      361 ccgatgtttt tagtaccgat ttcgctatat gttttgtaat ctaaaacttg taagggataa
      421 tctaagccgc ctaatgtgta ataagcggta aaaatagcac ttgggcgaat agtatcaata
      481 tcgccaccac tgcctattgt ataagaagtt gaaccgttta aagtatgcgt aattgttggc
      541 gtgttcttag ataagaaacg aaccgcaccc cacgaattaa gcatcatgtt taatgcttct
      601 aatgcgtcca tagactcatc agcagcagga gcttcacttg aactaatcgc gccgattaag
      661 cgcaaagacg agcgaattaa atcggacgtg accataagcc acctatagaa aaaagctcat
      721 ccttgagcaa cagaggttaa gctaattgat gaacggcacg aacagccaac tcaggataaa
      781 caggcgcata accgtaaagg atgtcgatac gagatttaaa ctcgcctgca tcaccgtcga
      841 accagcgaac aaaacgcata gatacaccgt cgtaaacttc acgagctgcc atatctacgc
      901 cgtttggcac ttctaagtca gcagtgacga aagcaaaagc gtctttgtgg aaagcgatgt
      961 tttgaccgta agcggttgca gaagtaccta acacagtgat tgctttgtta tcaccagcgc
     1021 gagcagttac gttttgataa gcaccaccag caataattgc agggctgatt gtcatagtaa
     1081 ttgcgccagc agtatcagaa atatcagcag ttacggtgaa tttctttaac ttgcctgtgc
     1141 tttgcttagt ttcgggatgc acttcgtaaa catcagcaat agtgataatg tcaccagctt
     1201 ttaagcttgt gcttgaccaa ccgtcagtaa ttaagcttga tgttgtagca taagcattat
     1261 cagcaccagt cgagccttgg tttgcgccat tggttaatgg tgtacctgca taagtacctg
     1321 tggtatgtgc aggaatcatt gtattttcaa acacatcaaa accaccagta cgacctacaa
     1381 taccctcttt gtattggttt tcaatgttag tagagctttg gaataagcct ttaaccgcat
     1441 cgctaaactc aacacgtgag gatggattca agataaaaca acgctgagag cttggtgcta
     1501 gattttcggt taatacttgg ccagcttgtt ggaatttttt gtaatctaac tgagtagata
     1561 cagtaccaac gtaattggga acgtacttat ataacgcatt gattgcatca tattcgattt
     1621 gagcagctaa ctgtgccatc gctggctcta aaatagtctt gctgaaatca tctaaagata
     1681 acgccatttc taaatcagaa atagccatgt caacgccttt gaccgttgcc acaggtaagt
     1741 tgactttgcg ctctactacg ttttgaccgc tataactcga gcctgtgcgg acagtgaact
     1801 tagcaggtaa gcgcaaatct aaagaagtac cgatttttgc accacgttta gcaaaacgat
     1861 catcatactg acgattcatg ttaccaacga tattaagttt ttggtgcaaa atacgttggg
     1921 cttcgcgtgt gataatttga gacgttaaaa ttgtattagc catgatttaa aactcacttc
     1981 tgacgtaatt gtttattacg atacgctacc cactctttca ttgataagct gttagggtca
     2041 acaattgagg attttccgct tactggctta acaggtggcg gcgcattcgt aacggttttt
     2101 ggtcttgtta aattagtttt tgctgcaatt tcaccaattg ccattaattg ttgcataggt
     2161 ggtaatgatg caatttgata cgcttttaca gggtctttac ccagcaaata agcgatttca
     2221 gcaccttttt catgccctgc aacagcatct agtgttgatt gtgcaaaagt aatttcagcg
     2281 acattggcaa atgcttcatc aaaatcaggt gcaacagctc ggactttctc aactttactt
     2341 tgccactctt gcgccacttt ttgagcttgc gcttgttggt tgtattgttg tgcctgtgct
     2401 tgtgtctttt gattgtactt atactcggct aaggcttcgg tataatcgtc aaaagtatca
     2461 aaatcaga
//
LOCUS       DAOQMB010000349         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26376_length_2466_cov_7.514309, whole genome shotgun sequence.
ACCESSION   DAOQMB010000349 DAOQMB010000000
VERSION     DAOQMB010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26376_length_2466_cov_7.514309"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLY05_09800"
     CDS             complement(<1..346)
                     /locus_tag="PLY05_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC03631.1"
                     /translation="MAEIKKVVLAYSGGLDTSVIVKWLQENYQCEVITFTADIGQGEE
                     VEPARAKAQAMGVKSIYIEDLREEFVRDFVFPMFRANAIYEGEYLLGTSIARPLIAKR
                     LVEIAQEQGADAI"
     gene            complement(422..1765)
                     /locus_tag="PLY05_09805"
     CDS             complement(422..1765)
                     /locus_tag="PLY05_09805"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC03632.1"
                     /translation="MLKETSAPTTSIQHPIAALSQNQLAHERVAIMTLLANRRRLGER
                     FAEELEDNYREFLVQRAISLMKSNKWIAVLFYLFLGLLTYQQVRYVSTFASLQHDLVI
                     WAMIYFSGAFIFSAIGICVANSKLDKHYAWYIGIASFVGLTSMSVAASSFDSIYINQQ
                     ASYVVIFIYMLIYSLGSLRIFTTLLIGLFSALLALTIIGTIGLPVEFGQFAQYAGLSN
                     MMGFLIASMLDQRDRRGFLQARLIDIEKQQLNQLSQEMARLSQEDGLTGLANRRHFNE
                     TLAREWAIAEREKQSIALIFIDVDHFKPYNDTYGHLEGDKVLSQVGKTLKKMAKRPAD
                     LAARYGGEEFVLLLPNTTVEGAFAVAEEVRQAIDDLAIPHKSSKAAKCVSVSIGLSAL
                     VPNGQNTITTLIDNADEGVYAAKKAGRHRIRIYPTPEEVNQRRETMLNHLNVKKT"
     gene            1899..>2466
                     /locus_tag="PLY05_09810"
     CDS             1899..>2466
                     /locus_tag="PLY05_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC03633.1"
                     /translation="MTTQLTITRPDDWHIHLRDGVALNNTVADVARYFGRAICMPNLV
                     PPVKNTEEALAYYQRIQAATPANSAFDPLMVLYLTDHTNAAEIAVAKASGKVKAVKLY
                     PAGATTNSHAGVTSLEKVYPVLEQLAEHNMPLLVHGEVTHSDIDIFDREKVFIDTILM
                     PLTQRFPTLKIVLEHITTADAVQFVQQAG"
BASE COUNT          761 a          535 c          424 g          746 t
ORIGIN      
        1 aaatagcatc tgcgccttgt tcttgagcaa tttcaactaa acgcttggca attaaaggac
       61 gtgcaatcga tgttcctaat aaatactcac cttcataaat ggcattagca cggaacatag
      121 gaaacacgaa gtcacgcaca aactcttcgc gtaaatcttc gatataaata cttttaacac
      181 ccattgcttg tgccttagca cgagcaggtt caacttcttc gccttgacca atatcagctg
      241 taaaggtaat tacttcacac tgatagtttt cttgtaacca ctttacaatt acagacgtgt
      301 ctaagccacc tgaataagct aagacaactt ttttaatttc tgccattttt tcttaccttt
      361 aagccttcga aaaaaacgcg ctattttacg tcatttacca cgttattatg aaagttttta
      421 tctaggtttt tttaacattt aagtggttca gcatggtttc acgacgctga ttaacctctt
      481 caggggttgg ataaatacga attcgatgcc gtcctgcctt tttagcggca tatacccctt
      541 catcggcatt atcaattaaa gtggtaatag tgttttgacc attaggcact aaagccgata
      601 aaccaatact caccgaaaca cattttgcag ctttagagct tttatgcgga atcgctaaat
      661 catcaatggc ttggcgtact tcttcagcaa cagcaaaagc cccttcgaca gtagtattgg
      721 gcaataacaa aacaaactct tcaccaccat aacgagccgc taaatcggct gggcgtttgg
      781 ccattttttt gagtgtttta cccacttggc ttagcacttt atcgccttct aaatggccat
      841 aggtatcgtt ataaggttta aaatggtcaa catctataaa aattaaggca atagattgct
      901 tttctcgctc tgcaattgcc cactctctgg ctaaagtttc attaaaatga cgacgattgg
      961 ctaagcctgt tagaccatct tcttgagata accgcgccat ttcttgacta agctgattga
     1021 gttgttgttt ttctatatca attaaacgtg cttgtaaaaa gccacgtcta tctcgttggt
     1081 ctaacataga ggcaattaaa aaacccatca tattagataa acctgcatat tgtgcaaact
     1141 gaccaaattc aacaggcaaa ccaattgtcc caatgatagt taatgctaat aaagccgaaa
     1201 acaaaccaat caataaggtt gtaaaaattc gcaaacttcc caaactataa atcaacatat
     1261 aaataaaaat aaccacataa ctggcttgct gattaatata aatactatca aatgacgagg
     1321 ctgcaacact catactggtc aagcctacaa aagaggcaat accaatatac caagcataat
     1381 gtttatccaa ctttgaatta gcgacacaaa taccaatcgc actaaaaata aatgccccac
     1441 taaaataaat cattgcccat atgactaaat catgttgcag tgaagcaaaa gttgagacat
     1501 aacgcacttg ctgataggtt aacaatccta aaaacaaata aaataaaacg gcaatccatt
     1561 tattagactt cattaagcta atggctcgct gcaccaaaaa ctcacgataa ttatcttcta
     1621 gctcctcggc aaaacgctct cctaagcgac gacgattggc taataatgtc ataatagcta
     1681 cacgctcatg cgctagttgg ttttgactta atgccgcaat agggtgctga atactcgttg
     1741 ttggcgcaga agtttctttt agcattattt gtctcttgtt tttagatggc ttttttattg
     1801 tgtttggtct gagattttta tgcgatataa tagctcatct tcatcgttgc taaagatagc
     1861 gcaatttttt attaatttca cttttaatct gataatttat gactactcaa ctcactatta
     1921 cccgtcctga tgactggcat attcacctgc gtgatggcgt ggctcttaac aatacagtcg
     1981 ccgatgtagc acgttatttt ggtcgggcaa tttgtatgcc caatcttgtt ccacctgtca
     2041 aaaacaccga agaagcctta gcgtattatc aacgcattca agcagcgacg cccgcgaaca
     2101 gtgcgtttga ccctttgatg gttttatatc tcaccgacca caccaatgcg gccgagattg
     2161 ctgttgctaa agcctctggc aaagtaaagg cagtcaaatt atatcctgcg ggtgcaacga
     2221 ctaactctca tgcaggcgta acctcattag aaaaagtgta tcctgtatta gagcaacttg
     2281 ccgagcataa tatgcctttg ttggtacatg gcgaagtcac gcatagtgat attgatattt
     2341 ttgaccgtga aaaagtattt attgatacga ttttaatgcc cttaacccaa cgttttccaa
     2401 cgctaaaaat tgtcttggaa catattacca ctgctgatgc tgtgcaattt gtgcaacagg
     2461 caggtg
//
LOCUS       DAOQMB010000350         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26570_length_2455_cov_6.545000, whole genome shotgun sequence.
ACCESSION   DAOQMB010000350 DAOQMB010000000
VERSION     DAOQMB010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26570_length_2455_cov_6.545000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PLY05_09815"
     CDS             <1..211
                     /locus_tag="PLY05_09815"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004279842.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNC03634.1"
                     /translation="GESFLDCINENALTVVQAVVEPSLATAQGEERFQFEREGYFVAD
                     LVDCQAGKLVFNRTVGLRDSWGGGA"
     gene            complement(251..850)
                     /locus_tag="PLY05_09820"
     CDS             complement(251..850)
                     /locus_tag="PLY05_09820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNC03635.1"
                     /translation="MTKANQDNPFERRLLNRVDSTLSLHYQPISSADSLKDPYDSNFS
                     LPRYFTLFAEIEQVDSVQRSQMQLLERENERLHGLFQAVNLKIELLTGAIYDGLAKLL
                     SPIPQRVNLSESGLSFHATEAITPGTHLHLAISHLEHHYHIAATARVVYCEDEDLEGF
                     RTGVYFININPSDRARLAQDIARKQQENEALKPFANPEE"
     gene            1034..2281
                     /locus_tag="PLY05_09825"
     CDS             1034..2281
                     /locus_tag="PLY05_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HNC03636.1"
                     /translation="MFKPVSLLIGLRYTRAKRRNHFISFISLTSIIGLVLGVAVLITV
                     LSVMNGFDRELKNRILGMIPHATISSREPFHDWPKLAEYAKHNPEVLAVAPFSQLQGM
                     LTANGQVTGALISGIDPVYEKQVSIIGQHIDRAKGQGSLDNLQANGFGIVLGEGLANN
                     LGVQLGDKLTLVLPEAAVSPAGVIPRFKRFTVVGIFKVGADVDGMLAYIHIADAGKLL
                     RIGDQVQGVRLKLHDIFQSRPVLLDLLSQLPSYFEGRDWTQTHGNLFSAIQMEKAMMA
                     LLLLLIVAVAAFNIVSSLVMVVTDKKADIAILRTLGASPQTITQIFMVQGAVIGLVGT
                     VGGVLLGIFLSLNISGFITWVEQVTGASLFDAYFINYLPSELKWSDVAWVSLVSALLS
                     FLATIYPARRAAKVQPAEALRYE"
     gene            2274..>2455
                     /locus_tag="PLY05_09830"
     CDS             2274..>2455
                     /locus_tag="PLY05_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC03637.1"
                     /translation="MSRIVLEAQGLCKSFKEEGMQGVDVLKNVNLTIERAEFVAIVGS
                     SGSGKSTLLHILGGLDV"
BASE COUNT          662 a          419 c          573 g          801 t
ORIGIN      
        1 aggcgagagc tttttagatt gtattaacga gaatgcttta accgttgtgc aggccgttgt
       61 tgaaccaagt ttggcaacag cgcaaggtga agaacgtttt caatttgagc gtgaaggcta
      121 ttttgttgct gatttagtcg attgccaagc aggtaagttg gtttttaacc gcaccgtagg
      181 cttgcgcgac tcttgggggg gcggagctta atgaggctca taaaaaaggc gaactgatgt
      241 tcgccttttt ttattcttca ggattagcaa aaggctttaa ggcttcgttt tcttgttgtt
      301 tgcgggcaat atcttgggct aaacgagcac gatcacttgg atttatatta ataaaataca
      361 cgcctgttct aaaaccttct aaatcttcat cttcacaata aacaacacga gccgtggcag
      421 caatatggta atgatgctct aaatggctaa tggctaaatg caagtgtgtt cccggtgtaa
      481 tggcttctgt ggcatgaaaa gacaaaccac tttcacttaa attaacacgt tgtgggatag
      541 gcgacagcaa cttagcaaga ccatcataaa tagcgcctgt taatagctca attttgaggt
      601 taacggcctg aaataaacca tgtaaacgct cattttcacg ttctaataat tgcatttggc
      661 tacgttgaac actatctacc tgctcaatct ctgcaaatag cgtaaaatag cgtggtaacg
      721 aaaagttact gtcataaggg tcttttaacg aatctgcact actaattggt tgataatgca
      781 gggatagtgt ggagtccaca cgattaagta agcgtctttc aaaaggattg tcttgatttg
      841 ctttagtcat gatatcccac tcttaaggca tgcttgccgt atttatgact gatattttat
      901 ttagttgatg atgcctttta caccaaacag tatgttggag taaagattat tatctaagca
      961 acatcgttta atttgttggt aatttggctt acttactgtg gtaagtcatt aatagtatgg
     1021 ttaaaatttt aacatgttca agcctgtttc gttactgatt ggtctgcgtt atacacgcgc
     1081 caaacgtcgt aatcatttta tttcttttat ttccttaacc tctattatcg gtttggtatt
     1141 gggtgttgcg gtgcttatta cggttttatc agtgatgaac ggttttgacc gtgaattaaa
     1201 aaaccgtatt ttaggcatga tacctcatgc cactattagc tcacgtgagc catttcacga
     1261 ttggcccaaa ttggccgaat atgccaagca taatcccgaa gtacttgctg ttgcaccatt
     1321 tagtcaatta caaggtatgt taacggccaa tggccaagtg acaggcgcac ttatttcggg
     1381 gattgatcct gtctatgaaa aacaagtgtc aattattggt cagcatattg atcgggctaa
     1441 aggtcaggga agtttagaca atttacaagc caatggtttt ggcattgtct taggcgaggg
     1501 attagcaaat aatttaggcg tgcaattggg tgataaatta actttagttt taccagaggc
     1561 ggctgtgtca cctgcgggcg tgattccccg ttttaaacgt tttaccgtgg ttggtatttt
     1621 taaagttggt gctgatgttg atggcatgtt ggcatatatc catattgctg atgcaggcaa
     1681 attgttaaga attggtgacc aagtacaagg agtgcgatta aagctacatg atatttttca
     1741 atcacgtcct gtattgttag atttactcag ccaactaccc agttattttg agggcagaga
     1801 ttggacacaa acgcatggca acttgtttag tgcgattcaa atggaaaaag ccatgatggc
     1861 cttactccta ttattaattg tggcggtggc ggcttttaat atcgtctcta gtttagtgat
     1921 ggtggtaaca gataaaaaag cagatattgc catcttgcgt actttgggtg cttcgccaca
     1981 aaccattact caaattttta tggtgcaagg ggcagttatt ggtttagtgg ggacagtggg
     2041 cggtgtgttg ttaggtattt tcttatcatt aaatatcagt ggttttatta cgtgggttga
     2101 gcaagtgaca ggtgcttcgc tgtttgatgc ctattttatt aattatttgc cctcagaatt
     2161 aaaatggagt gatgtggcat gggtcagttt ggtgtctgca ttattgagct ttttagcaac
     2221 catttatcca gcaagacgcg cggccaaagt acaacctgca gaggctttac gttatgagta
     2281 gaatcgtttt agaggcacaa ggtttatgta aatcctttaa ggaagaggga atgcaagggg
     2341 ttgatgtatt aaaaaatgtc aatttaacca ttgagcgtgc agaatttgtg gcgattgtcg
     2401 gcagttctgg ttcaggcaaa agcacgttgt tgcatatttt ggggggcttg gatgt
//
LOCUS       DAOQMB010000351         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26607_length_2453_cov_15.709758, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000351 DAOQMB010000000
VERSION     DAOQMB010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26607_length_2453_cov_15.709758"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1102)
                     /locus_tag="PLY05_09835"
     CDS             complement(2..1102)
                     /locus_tag="PLY05_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HNC03638.1"
                     /translation="MLNKALNIANQWALQKVSRDWIDYIASNTMTDFNRWFNDFIWTY
                     DLKAMVTAIETQADGVKTIHLLPNQHWKTYQAGQFVELVLEIDGQPHHRYYSLSPMTT
                     KGTFSITVKKQADGVVSSYLTEQLQVGQSVKLNLPQGDFVYKQQQKLLFICAGSGITP
                     CYSIISDLLAQNKAVDLAIYAQFSKQKDVIFANSLKQQWPAQGVSVEVAFTQETDSQH
                     WLLTAENLLVQYPDLLERDVYLCGPTGFMDNMINALVAANFNLAKLHCERFVTVENIE
                     SGNLDFKVVQPSIYFKHLNQHIQLTTEDEGKSLLQIARQYGVNLESGCQKGMCGTCKL
                     TLKEGKIAGNQLGNAVYLCTSYPDSARIVLDA"
     gene            complement(1208..2350)
                     /locus_tag="PLY05_09840"
     CDS             complement(1208..2350)
                     /locus_tag="PLY05_09840"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HNC03639.1"
                     /translation="MVETLNKPQPIKQQNTVVRDDAFYDQIEQEFEAIKQRHRAKMGA
                     EDVAYIKGLRLKSRIFEVLGRGLLWTSRNPLTFGAGVGFLFLHRNIEAIEIGHNVLHG
                     QYDYFPEIPQFHSHNFKWKAPIDEEGWRREHNAMHHVHTNVYEKDPDLNHGLLRTNDL
                     VPWQKSHLWQVPLYFGVVYPTMLYRFNAQNLGFTQEYRAEAFDTGNQGYAVVDTSYAS
                     KEQREKMHSRSVNRVLFKEYLVFPLLAKLTGRSALKVLAGNFLADTINNYWIGMTIQA
                     THFTEDLQDEAAVDHKGRWYLSQLESSVNFKGNRWQSILWGHLNYQIEHHLFPDTPSH
                     RYPDMAKDVKALCQKYNLTYKCNPSWAKAIKNYTAVFWKYSFPTKK"
BASE COUNT          760 a          512 c          447 g          734 t
ORIGIN      
        1 tttaagcatc caacacaatt cttgccgaat caggataaga ggtacacaaa tacaccgcat
       61 tacccaactg attgcccgca attttgcctt ctttaagggt cagcttacac gttccacaca
      121 tacccttttg acaaccagac tccaaattaa ctccatactg acgagcaatc tgcaataaac
      181 ttttgccttc gtcttcggtc gttaactgaa tatgttgatt gagatgttta aaataaatgc
      241 tcggttgcac cactttaaaa tctaaattgc cagactcaat attttcaacc gtaacaaaac
      301 gctcacaatg caatttggct aaattaaaat tagcggccac caaagcatta atcatgttat
      361 ccataaagcc tgttggccca cacaaatata catccctttc taacaagtca gggtactgaa
      421 ccaataaatt ttcagcagtt aataaccaat gttgtgagtc ggtttcttga gtaaatgcaa
      481 cttcaacaga aacgccttgt gctggccatt gttgctttaa actattggca aaaatcacat
      541 ctttttgctt agaaaactgg gcataaattg ctaaatcaac ggctttattt tgtgctaata
      601 aatcactaat aatcgaataa caaggggtaa tgcccgaacc agcacaaata aacaataatt
      661 tttgctgttg cttataaaca aagtcacctt ggggtaaatt gagtttgaca ctctgcccca
      721 cttgtaattg ctcagttaaa taactcgaca caacaccatc cgcttgtttt ttaacagtaa
      781 tgctaaatgt gcctttggtg gtcataggag acaaactgta ataacgatga tgcggctgac
      841 catctatctc aagcaccaac tcaacaaact gacctgcttg atacgttttc caatgttgat
      901 tgggtaataa gtgaatggtt tttaccccat cagcttgcgt ttcaatggcg gtgaccatcg
      961 cttttaaatc gtaggtccaa ataaaatcgt taaaccaacg attaaaatcg gtcatggtat
     1021 tgctggcaat ataatcaatc caatcacggc taactttttg taatgcccat tgattggcaa
     1081 tatttaatgc tttgttgagc ataaaaaatc ctaaaagtct aatcaaagtt tgatttagcc
     1141 ctcaccccaa ccctcttcca cagggagagg gtgttatata tgagagagga ctcatgtcag
     1201 ggaaagctta ttttttagtt ggaaatgaat atttccaaaa tacggcggta tagtttttaa
     1261 tggcctttgc ccaactggga ttgcacttat aggttaagtt atatttttgg cacagagctt
     1321 tgacatcttt agccatgtcg ggataacggt gtgaaggcgt atcgggaaat aaatggtgtt
     1381 cgatttgata attcaaatgt ccccacaaaa tgctttgcca acgattgcct ttaaaattta
     1441 ctgaggattc taactgcgat aaataccaac ggcctttgtg gtcaacggct gcttcgtctt
     1501 gtaagtcttc ggtaaagtgt gtggcttgaa tggtcatgcc aatccaataa ttgttaatgg
     1561 tatcggctaa aaaattacct gctaacactt ttaaagctga acgacctgtc agtttcgcta
     1621 acaaaggaaa aaccaaatat tctttaaaca atacgcgatt caccgagcga gagtgcattt
     1681 tttcgcgttg ttctttgctg gcataactgg tatcaacgac ggcataacct tgattacctg
     1741 tatcaaaggc ttcggcacga tattcttggg taaaacctaa attttgtgcg ttaaaacgat
     1801 ataacatcgt tggataaacc acgccaaaat ataacggcac ttgccacaga tgcgactttt
     1861 gccacggtac taaatcgtta gtacgcaaca aaccatgatt gaggtctggg tctttttcat
     1921 agacattagt atgaacgtgg tgcatggcat tatgctcacg acgccaacct tcttcatcaa
     1981 tcggtgcttt ccacttaaag ttgtgggaat ggaattgggg aatttcgggg aaataatcat
     2041 attgaccatg caacacattg tggccaattt cgatggcttc gatgttgcga tgtaaaaata
     2101 gaaaaccaac acccgcacca aaggttaagg gattacggct tgtccacaat aaaccacggc
     2161 ctaacacctc aaaaatacgt gactttagac gcaacccttt aatataggcc acatcttccg
     2221 cccccatttt tgcgcgatgg cgttgtttaa tcgcttcaaa ttcctgttca atttgatcat
     2281 aaaaagcatc atcacgaaca actgtatttt gttgtttaat gggctgtggt ttattaaggg
     2341 tttctaccat agttgcgacc taaactaaac tatcaataaa aaaagccgct tattctaatg
     2401 ctttctagct taaaaaaacg catatatccc gaaaatttgt gtacaaatgt ata
//
LOCUS       DAOQMB010000352         2451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26653_length_2451_cov_6.983306, whole genome shotgun sequence.
ACCESSION   DAOQMB010000352 DAOQMB010000000
VERSION     DAOQMB010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26653_length_2451_cov_6.983306"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="PLY05_09845"
     CDS             <1..626
                     /locus_tag="PLY05_09845"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNC03640.1"
                     /translation="VKFFAPVHEGQKTGWFYDHRESRAQLQRVVKGKRVLDVFCYAGS
                     WGVQAAAFGAAEVTCVDASEFALNIVQKNAQANGLENIKTLQGDAFTMLTQLKQQGEQ
                     FDVIVLDPPAFMQKRKDHANGLQAYRRINELAMRLLVRDGILVSGSCSMHLKTEELVD
                     VIRGASRHLDKTAQVVFIGGQGADHPVHPAIPETSYLKCVMARVLGA"
     gene            complement(662..>935)
                     /locus_tag="PLY05_09850"
     CDS             complement(662..>935)
                     /locus_tag="PLY05_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03641.1"
                     /translation="SFDLTIETIVSGLGSIPIPTVTIQNIPKPETQDIFCNDDEVNDA
                     LPDGAITITACSFSGNVGTISVQLNQGILLNYTVKYTYTPTSSIRS"
     assembly_gap    936..1035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1036..2003)
                     /locus_tag="PLY05_09855"
     CDS             complement(<1036..2003)
                     /locus_tag="PLY05_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03642.1"
                     /translation="MNKSHLLGGGLTLLASLMLAACNSNDDDNAAIPAVVQTTQVTVT
                     PSLGKILNAKVVLKNAKTGATLGNGNTGSTGIATFTATKTSDPVVVEVQGVDGAQGAT
                     YFDEAKNANIPLPATQKIRAIVPTLADNANVGVTALTEVATQAAIKAAGDLSKITTQI
                     ATTANDQVKTLLAKELGSASLLTPPTIIGKDTIVKNVITARNAANDYALKLAALAKLG
                     QGNTPVLDLLQKLSDDLSDNKLDGMKGSTDIDYSTGETADITDALQAYIAAYANAAQI
                     NTIYTSAILNSFAVTEGTITITITNGSSGSGSGSGSGSGSGSGSGSG"
BASE COUNT          663 a          425 c          508 g          755 t
ORIGIN      
        1 gcgttaaatt ttttgcgcct gtgcatgaag gacaaaaaac gggttggttt tatgaccacc
       61 gtgaatcacg cgcccaatta caacgggtag tcaaaggcaa acgtgtgtta gatgtgtttt
      121 gttatgcagg cagttggggc gtgcaagccg ctgcttttgg tgcggcagaa gtgacttgtg
      181 ttgatgcctc tgaatttgcc ttaaacattg tacaaaagaa tgcccaagct aatggcttag
      241 aaaatataaa aaccttacag ggtgatgcct ttacgatgtt aactcagctt aaacagcaag
      301 gcgaacagtt tgacgtgatt gtgttagacc ctccagcgtt tatgcaaaaa cgtaaagacc
      361 atgccaatgg tttacaagcc tatagacgca ttaatgaatt ggcgatgcgt ttattagtgc
      421 gtgatggtat tttggtgtct ggctcttgtt cgatgcactt aaaaaccgaa gagttggttg
      481 atgtgattcg tggtgcaagt cgtcatttag ataaaaccgc gcaagtggta tttattggcg
      541 ggcagggggc tgatcatcct gttcatccag ccattcctga aacatcctac ttaaaatgtg
      601 tgatggcaag ggtgttgggg gcttaaataa aaaagggcat cgaaagatgc cctttttggt
      661 gttaggatct aatagaggat gtgggagtat aagtgtattt gacggtataa tttaacaaaa
      721 tcccttgatt taattgaact gaaatagtac caacattacc agaaaaggaa caagcagtaa
      781 tagtgattgc tccatctggc agggcatcgt tgacttcatc atcattacaa aatatatctt
      841 gtgtttctgg tttagggatg ttttgtattg tgactgttgg aataggaatg ctacctagtc
      901 cagaaacaat agtttcaatg gtaagatcaa aactannnnn nnnnnnnnnn nnnnnnnnnn
      961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnccgct accgctaccg ctaccgctac cgctaccgct accgctaccg
     1081 ctaccgctac cgctactacc attggtaatc gtaatagtaa ttgtgccttc tgtgactgca
     1141 aaactattta aaatggcaga ggtataaata gtattgattt gtgcggcatt tgcataagcc
     1201 gcaatatagg cttgtagtgc gtcagtaata tcagcagttt cgcctgtaga gtaatctata
     1261 tctgtgctgc ctttcatgcc gtctaactta ttgtcactaa ggtcatcaga aagtttttgt
     1321 aataaatcaa gtacaggtgt attgccttgc cccaattttg ccagtgctgc cagttttaaa
     1381 gcatagtcat tagccgcatt acgtgcggta attacatttt taacaatagt gtctttgcca
     1441 ataatggtgg gtggcgttag aagggaagca gagcctagct ctttggctaa taatgtttta
     1501 acttgatcgt ttgctgtagt ggcaatttgt gtggtgattt tagacaaatc accagcggct
     1561 tttatggcag cttgtgtggc cacttcggtt agcgcagtca caccaacatt cgcattatcc
     1621 gcgagcgtgg gaacaatagc tcgaattttt tgtgtggcag gtaatggaat attggcattt
     1681 ttggcttcat caaagtatgt tgcaccttgc gcaccatcaa cgccttgaac ttcaacgaca
     1741 acagggtcag aggtttttgt tgccgtaaat gtagcaatac ctgtactgcc tgtattacca
     1801 ttacctaaag tcgcgccagt tttagcgttt tttaatacca ctttggcatt taaaattttg
     1861 cctaaagagg gagtaactgt cacttgtgta gtttgtacta cggcaggtat agcagcatta
     1921 tcatcatcat tactattaca ggcagctaac attaaactcg ctaataaagt taaaccacca
     1981 cctaataagt gagatttatt catattttat ctccatgtta tcttaagtta ttgttatata
     2041 agattaaaaa ctaaatctat ataaaatata gctttcttag gacttacacg gctgtcgctt
     2101 atttgttcgc taaacaatcg ttttaaacga tttttaactc acaaaacgcg ctaatttcga
     2161 tgaaatgtgt aagtcttttt tctgttgaaa atctaagcta ttttttgagt atatctatgc
     2221 ttattattga aaacaatatc agaagtaaaa ttacaaaaaa tatttaatta ttgtgaaatg
     2281 ttgttgatac tcattatttt taggacttac tccatgtagc agtgagtcct aattaagcgt
     2341 tgtcgttagg cagaaataat ttttggagta gatttggggt gttgagtgct tgtttgagac
     2401 cgcttattgc tactcaccga tttttgacgc ggcatagagc tattgccaac c
//
LOCUS       DAOQMB010000353         2447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26758_length_2447_cov_4.229097, whole genome shotgun sequence.
ACCESSION   DAOQMB010000353 DAOQMB010000000
VERSION     DAOQMB010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26758_length_2447_cov_4.229097"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLY05_09860"
     CDS             <1..230
                     /locus_tag="PLY05_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03643.1"
                     /translation="NGYLGSDYGSDAKSLLQKALHSGVADAFIEKMKKDLPILSVIPQ
                     ENIGLYSIPEPPDKLRLFIAIDGITSVEITP"
     gene            227..2344
                     /locus_tag="PLY05_09865"
     CDS             227..2344
                     /locus_tag="PLY05_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03644.1"
                     /translation="MTTKKEFLAQADIELLNPQYGKIAELKQAQDPFVVAQIGAMAQM
                     LELYSTQQNIAETEVFLKARDATILADATLKGILPLANCAKVTVSVVNPSLTNSVTLA
                     YGRRLLDDKGRVWRVASTLVVAANSTGTVQLHQNEIRTVTHTIATSEPFYSIVIPKNE
                     EALHLENIRIKDTIGNTDNLYRYAPEFMNVLNGERVYHLETDESRQINIRLGAQDGAS
                     LVYGFQPPVNTVLAIELTETQGLLDIALNASFSLEYSNNINDDNLKITVSAINSLGAN
                     PLSLSVLSVLSRYAALYDHNAVYLSDFDYLIRRYHGDSINFLAVWNEQIHEQAYGYSV
                     ANINKLFIAVQAKNSLEQANIVNEIHTLIQRADNSYKRQIVTVIEQPYPLAITASVAA
                     IHDSVQVEQQIKDTLLAIYGKGQIKVSQGLKNNFNRQEIYQTLKRAIPALQDSISDFN
                     VVVDSNLLPNPVLPAHYFYLHSSTNFSVSHAIKRCRRWTMELTPLAPLQASAEFWDDI
                     DETSIESELKQLFIALFEHFLRTKFRRIDSYGYPHLLDNADFETIERFVKLDGLSLLN
                     RETNNQPYMREVFRAWRGQHQRRGLGFLEFYLQMLWPNAWEIKQQYHPIATENNYPAN
                     ITLSDLGNSFLTSRVSVLLDPTQLTNSNEIPKMIPSLKRVVPARIVLNVAIGISVEPP
                     VDMNIGMAFTPTFYMSLEDGAVV"
BASE COUNT          777 a          476 c          484 g          710 t
ORIGIN      
        1 ctaatggcta tttaggtagt gattacggca gtgatgcaaa gtcgttatta caaaaagcct
       61 tacattcagg cgtagctgat gcttttatcg aaaagatgaa aaaagactta ccaattttga
      121 gtgtgatacc acaagaaaat atcggcttgt attctattcc tgagccgccc gacaaattac
      181 gcttatttat tgcgatagat ggtattacta gcgtagagat taccccatga ccactaaaaa
      241 agagttttta gcgcaagccg atattgaatt acttaatcct caatacggca aaattgcaga
      301 gttaaaacaa gcacaagacc cttttgtggt tgcccaaatt ggtgcaatgg cgcaaatgtt
      361 ggagctatac agcactcaac aaaacattgc cgaaactgag gtatttttaa aagcgcgtga
      421 tgcaacaatt ttagccgatg ctacgctaaa agggatttta ccccttgcaa attgcgccaa
      481 agtcacagta agcgtggtta atccaagttt aacaaacagt gttaccttag cttatggcag
      541 acgtttatta gatgacaaag gccgtgtatg gcgcgtagcc tcaacattag ttgttgctgc
      601 aaacagcact ggtacagtgc aattacatca aaacgaaata cgcaccgtta ctcacaccat
      661 tgctactagc gaaccgtttt acagtattgt catacctaaa aacgaggaag ccttacactt
      721 agaaaatatc agaattaaag acaccatagg caatacagat aacctatacc gctatgcacc
      781 tgagtttatg aatgtgttga acggtgaacg tgtttatcat ttggaaacag acgaatcccg
      841 acaaattaac ataagactag gcgcacaaga cggagcaagc cttgtttacg gttttcaacc
      901 gccagttaat accgttttag cgattgaatt aaccgaaaca caaggtttgt tagatattgc
      961 gttaaatgct agttttagcc tcgaatatag caataacatt aatgacgata acctaaaaat
     1021 tacagtcagt gcgattaact cattaggtgc gaatccctta tcattgtcag tattgagcgt
     1081 gttatcacgt tatgcagcat tgtatgacca taacgcagtc tatttatcgg attttgatta
     1141 tttaattaga cgctaccatg gcgacagtat taactttttg gcggtgtgga acgagcaaat
     1201 acacgaacaa gcctatggtt acagtgtagc caacatcaat aaattattta ttgccgtaca
     1261 agctaaaaac agcttagagc aagccaacat tgtcaatgag attcacacct taatacagcg
     1321 agctgataac agttataaac ggcaaattgt tacagttatt gagcagcctt atcctttggc
     1381 cattaccgca agcgtggcag caattcacga tagcgtacag gttgagcaac agattaaaga
     1441 taccttatta gcgatttatg gaaaggggca aattaaagta tcgcaagggc ttaaaaataa
     1501 ctttaatcgt caagaaattt accaaacctt aaagcgtgct attccagctt tgcaggattc
     1561 aatcagcgat tttaatgtgg tggttgacag taatttgcta cctaatccag tcttgccagc
     1621 gcattatttt tacttacact caagtaccaa ttttagcgtc agtcacgcaa ttaagcgatg
     1681 caggaggtgg actatggaat taacgccttt agcaccatta caagccagtg ctgagttttg
     1741 ggacgatata gacgaaacca gtattgaatc tgagcttaaa cagttgttta ttgctttgtt
     1801 tgagcatttt ttacgcacca agtttagacg tattgatagt tacggctatc ctcatttatt
     1861 agataacgct gattttgaaa ccatagaacg ctttgtcaaa cttgacggat tgagtttatt
     1921 aaaccgtgaa accaataatc aaccctatat gcgcgaagtg tttagagcat ggcgcggaca
     1981 acatcaaagg cgaggtttag gttttttaga gttttacttg caaatgcttt ggcctaatgc
     2041 gtgggaaatt aaacaacagt atcacccaat tgcaacagaa aataactacc cagctaacat
     2101 tacattgtcc gatttgggta atagtttttt aaccagtcgt gtgtcagttc ttttagaccc
     2161 aacacaatta accaactcaa acgaaatacc taaaatgatt ccgtccttaa agcgtgttgt
     2221 gccagcgcgt attgtgctta atgtggcgat tggtattagt gttgagccgc ctgtagatat
     2281 gaatatcggt atggcgttta cacctacgtt ttatatgagc ttagaagatg gtgcagtggt
     2341 ttgagtgtgt aaccacgcaa aaacccctat caaaactcat tcataatagc tttaacttaa
     2401 ccgttaatga gattttatta tgtctgtctc cccagccgat ttattag
//
LOCUS       DAOQMB010000354         2444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26805_length_2444_cov_14.789870, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000354 DAOQMB010000000
VERSION     DAOQMB010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26805_length_2444_cov_14.789870"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..955)
                     /gene="trpA"
                     /locus_tag="PLY05_09870"
     CDS             complement(146..955)
                     /gene="trpA"
                     /locus_tag="PLY05_09870"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNC03645.1"
                     /translation="MSRIASCFQNLAKQNKAALIPYITAGDPHPRYTVSMMHALVAGG
                     ADIIELGVPFSDPMADGPTITKAHERALVHNTSSHDVLAMVKEFRQSNQQTPIVLMGY
                     LNPLEIIGYEAFAADAQQAGVDGILTVDLPPEESECLVAALKAHQLDPIFLLAPTTTD
                     DRIASITAAASGYVYYVSLKGVTGSNALDVESVKERIATIRQFSQLPIGVGFGIKDAQ
                     SAAEVARCANGVVVGSVLVNRIAELQQTPELIPAQLQAIMSEMRQAMDATV"
     gene            complement(958..2169)
                     /gene="trpB"
                     /locus_tag="PLY05_09875"
     CDS             complement(958..2169)
                     /gene="trpB"
                     /locus_tag="PLY05_09875"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004732965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNC03646.1"
                     /translation="MTAINFSNFPDARGHFGVHGGRFVSETLMAALLDLEKLYARLKD
                     DANFRAEFDRDLAFYVGRPSPLYHAERWSRELGGAQIYLKREDLNHTGAHKVNNTIGQ
                     ALLAKHSGKTRVIAETGAGQHGVATATIAARLGMECVVYMGAEDVKRQAMNVYRMKLL
                     GATVVPVTSGSKTLKDAMNEAMRDWVTNVDNTYYVIGTVAGPHPYPMLVRDFQSIIGR
                     EARQQCLAQTGRLPDALVACVGGGSNAIGLFHPFLTDEAVAMYGVEAAGDGIDSGRHS
                     APLSAGKIGVLHGNRTYLMTDPNGQIIETHSISAGLDYPGVGPEHSWLKDIGRVNYVA
                     ITDDEALNAFRHVTRTEGIIPALESSHALAYTHKLAPTLAKDKIIICNLSGRGDKDLL
                     TVAKLDGIEVV"
     gene            complement(2166..>2444)
                     /locus_tag="PLY05_09880"
     CDS             complement(2166..>2444)
                     /locus_tag="PLY05_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-(5'-phosphoribosyl)anthranilate isomerase"
                     /protein_id="HNC03647.1"
                     /translation="AKIAEYPQASGILLDAWHPQLKGGTGESFDWRHWPQSTKPLILA
                     GGLTPENVSEAIHLTQAYAVDVSGGVEVSKGIKEFTLLQAFMAGVTAA"
BASE COUNT          719 a          601 c          466 g          658 t
ORIGIN      
        1 ttttacctgc attgggcaaa atttttccta accagctcat agaaaaactc tctttaagtt
       61 aatttattcc ctcaccccaa ccctctccca cagggagagg gagaaagcag ggagttaccc
      121 cttacttaac aagggtgatg aggatttaaa cagtagcgtc catggcctga cgcatttcac
      181 tcattatagc ttgtaattga gcaggaataa gctctggtgt ttgctgtaat tcagcaatac
      241 gattaaccaa tactgaacct acaaccaccc cattagcaca acgcgcaact tcggctgccg
      301 attgagcatc cttaatacca aaaccgaccc caattggtaa ttggctaaat tgacgaattg
      361 ttgcaattcg ctctttaacc gattccacat ctaaagcatt agaacctgtt acgcccttta
      421 acgacacata atagacataa ccgctcgctg ccgcggtaat gctagcaata cgatcatcag
      481 tagtagttgg tgctaataag aaaatcgggt ctagttggtg tgcttttaaa gcagccacta
      541 aacattcact ttcttcgggc ggtaaatcta ccgttaaaat gccatccaca cctgcttgtt
      601 gggcatcagc cgcaaaagct tcataaccaa tgatttctaa agggtttaaa tagcccatta
      661 acacaattgg tgtttgttgg ttgctttggc gaaactcttt gaccatcgct aacacatcat
      721 gactgctagt attatgcacc aaagcacgtt catgagcttt agtaatcgtt ggaccatcag
      781 ccattggatc agaaaatggc acacccaatt caataatatc agcaccaccc gctaccaacg
      841 catgcatcat actcacggta taacgcggat gtggatcacc tgcggtaata taaggaatta
      901 aagcggcttt attttgcttg gctaagtttt gaaaacagct tgcgattctg gacataatta
      961 caccacttcg attccatcta attttgcaac agttaataag tctttatcac cacgtccaga
     1021 caaattacaa ataatgattt tatctttagc caacgttggg gctaatttgt gggtataggc
     1081 taaggcatga cttgattcta aggcaggaat aataccttcg gtacgtgtga catgacggaa
     1141 agcatttaac gcctcatcat cggtaatcgc cacataattt acccgaccaa tatcttttaa
     1201 ccagctatgc tcagggccaa caccagggta atcaaggcca gcagaaatag aatgggtttc
     1261 aatgatttga ccatttggat cggtcattaa ataggtacga ttaccatgta acacaccaat
     1321 tttccctgca cttaaaggcg cagagtgacg acccgaatca ataccatcac cagctgcttc
     1381 aacgccatac atcgccaccg cttcatcggt taaaaatgga tgaaacaaac caatcgcatt
     1441 agaaccgcca cccacacacg ccactaaagc atcaggtaaa cgacctgttt gtgctaaaca
     1501 ttgttgacgc gcttcacgac caataatgga ttgaaaatca cgaactaaca ttggataagg
     1561 atgtgggcca gccactgtac caatcacata gtaagtatta tcaacattcg ttacccagtc
     1621 acgcatggct tcattcatag cgtctttgag ggtttttgaa ccagaggtta caggcacaac
     1681 ggttgcacct aaaagcttca tgcgataaac gttcattgct tgacgtttaa cgtcttctgc
     1741 gcccatatac accacacatt ccatgcctaa acgtgcggca atggtggcag ttgccacacc
     1801 atgctgacct gcacctgttt cggcaatcac acgggttttg cccgaatgct tagccaacag
     1861 cgcttgacca atggtgttat tcactttatg tgcgcctgta tggtttaagt cttcgcgctt
     1921 taaataaatt tgcgcgccac ctaattcacg gctccaacgc tcagcatgat ataacggtga
     1981 aggacggcca acataaaaag ctaagtcacg gtcaaactcg gcacgaaaat tggcatcatc
     2041 ttttaaacgt gcatataact tttctaaatc taataaagcg gccattaatg tttcagacac
     2101 aaaacgacca ccatgtacac caaaatgacc acgcgcatct gggaaattgc taaaatttat
     2161 cgcagtcatg ctgcagttac tcccgccata aaggcctgta ataaggtaaa ttctttaatg
     2221 cctttgctaa cttctactcc accactgaca tccaccgcat aggcttgggt cagatgaatg
     2281 gcttcgctga cattttctgg ggttaaacca cccgctaaaa tcaggggctt ggtgctttgt
     2341 ggccaatgtc gccaatcaaa actttcaccc gttccacctt ttaattgtgg atgccatgcg
     2401 tctaataaaa taccgcttgc ttgaggatat tccgctattt tggc
//
LOCUS       DAOQMB010000355         2439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26902_length_2439_cov_4.380453, whole genome shotgun sequence.
ACCESSION   DAOQMB010000355 DAOQMB010000000
VERSION     DAOQMB010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26902_length_2439_cov_4.380453"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..471
                     /gene="csx16"
                     /locus_tag="PLY05_09885"
     CDS             181..471
                     /gene="csx16"
                     /locus_tag="PLY05_09885"
                     /inference="COORDINATES: protein motif:HMM:NF021190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HNC03648.1"
                     /translation="MTVYFVSRHAGAKEWAALQGLTCDVCVPHLDPVLVKEGDVVIGT
                     LPINLAAIVCEQGGRYFHLSLELPLEARGKELSPSDLQKYGAKLEEFVVRRG"
     gene            complement(458..1663)
                     /locus_tag="PLY05_09890"
     CDS             complement(458..1663)
                     /locus_tag="PLY05_09890"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HNC03649.1"
                     /translation="MSRVMLIIATGQNAANIQPLSEPSLKPDSVCIAVTKDMEKHYEE
                     LKNQIGALGIKVEPPLVLQQELNVLSLEDTFSHWLLDKHSDNEVFVNLAGGLKTITVA
                     AYKTFSALGAKVFYAHYDGQIVWLDIPQEAALRITLTPNIQLAAYLKIYQYEIINKQT
                     IANIAYEHKQYAHGIATYLRHHFESRCAAISHLNWLAHPIIEQVNKSEFSSKNKSHQN
                     QPILYIENPKTNSQIHELYQAIPYIEKIFSYTPKRLTFSSAESAKIVGGGWLDILVAE
                     TLRELTELNFRDISVNVSFSKSTQRKGKQTHNELDVMALYGTHLLIVECKTINWDSNK
                     EASPLESIYKLAALADIGGLNTHAVFISLYELPANAKTRAAENNIIVIDGKKLLDLPA
                     LIKQGINPA"
     gene            complement(1697..>2439)
                     /locus_tag="PLY05_09895"
     CDS             complement(1697..>2439)
                     /locus_tag="PLY05_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNC03650.1"
                     /translation="LQGSWGEQKVDLLLERAGLRKNIEYSKEENFKNDEGKNQRPDFV
                     VKLPEGKQIIVDSKMSLVAYMEYVAADNDKARQEALSKHIKAVEFHINDLGSRQYHTL
                     QGLNTPDFVFLFMPIEPAYILISEHAPELFEKAYEKNIAIVTAANLLPVLRVVANLWS
                     IQRQNQTTKELADQASLVYNKLRIFIEKMEDLGKHIDKAQVSYRESFNSLKDGQGSLT
                     KTVNKFVELGVKVNKQLPSSVLIEDLGE"
BASE COUNT          726 a          445 c          461 g          807 t
ORIGIN      
        1 gacgttgagg cataaccgca tactccttag aaagagcaga attagcatca agaaacttga
       61 gggatgtatg gatgatagat tgaattttag gcaggttgtt gtttcataag cttgcaaaat
      121 cgcactttgc aattagcctt aatttatagt tgccttactt aatttagagt tgattacaca
      181 atgactgttt actttgtttc tcgtcatgct ggtgcaaaag aatgggcagc tttacaaggt
      241 ttgacctgtg atgtatgcgt gccgcattta gaccctgttt tggtcaaaga aggagatgta
      301 gtgattggta ctttacccat taatttagcg gcaattgtgt gcgagcaggg cggacgttat
      361 tttcatttaa gtttggagtt gcctttagag gcacgcggta aagagttaag ccccagtgac
      421 ttgcaaaagt atggggctaa gttggaagag tttgtggtta ggcggggttg ataccttgct
      481 taatcagcgc aggcaagtct aaaagttttt tgccatctat aacaataata ttattttcgg
      541 cagcacgagt tttggcatta gcaggtaatt catacaaaga aataaaaacg gcatgggtat
      601 ttaaaccacc aatatcggct aatgcggcta atttataaat agactctaaa ggcgatgcct
      661 ctttattact gtcccaatta atcgttttgc attcaacaat taaaagatgc gtgccataga
      721 gtgccatgac atctagttca ttatgtgttt gtttgccttt acgttgcgta cttttgctaa
      781 atgacacatt aaccgaaata tccctaaagt ttagctctgt taattcgcgc aatgtttcag
      841 ctactaaaat atcaagccat ccgccaccta caatttttgc agattcagca gagctaaacg
      901 ttaaacgctt gggtgtataa ctaaatattt tttctatgta gggaatggcc tgatatagct
      961 catgaatttg tgagtttgtt ttaggatttt ctatataaag aatgggttgg ttttgatgag
     1021 acttgttttt ggatgaaaac tcagacttat taacttgctc aataataggg tgagccaacc
     1081 aattaagatg gctaattgca gcacatcgac tctcaaaatg atgacgtaaa taagtagcta
     1141 ttccatgtgc atattgttta tgttcataag caatattagc aatggtttgt ttgttgataa
     1201 tttcatattg atagattttt agataagcag ctaattgaat attgggcgta agcgtaatac
     1261 gcaaagcagc ttcttgagga atatctaacc aaacaatttg gccatcataa tgcgcataaa
     1321 aaacttttgc accaagtgcc gaaaaggttt tataggctgc tacagtaatt gtttttaggc
     1381 cgccagccaa attaacaaaa acctcattat cactatgttt gtcgagtagc caatggctaa
     1441 aggtatcttc taaagataaa acatttaatt cttgttgtag aactaaaggg ggttcaactt
     1501 tgatacctaa agccccaatt tgatttttta attcttcata atgtttttcc atatctttgg
     1561 taacggcaat gcaaacacta tcaggcttta gactcggctc acttaatggc tgaatgttgg
     1621 cggcattttg gccagtagca ataattaaca tgacacgtga cataatttta ctccctcaca
     1681 taattctaaa aaatagttac tcacccaagt cttctatcaa cacactagac ggtaattgct
     1741 tattgacttt tacgcctagt tcaacaaatt tgttaacggt tttagttaag ctaccttgac
     1801 cgtcttttaa gctattaaac gactctcgat agcttacttg agctttatca atatgtttac
     1861 caaggtcttc catcttttcg ataaaaatgc gtaacttgtt ataaacgagg cttgcttgat
     1921 cggctaactc tttggtggtt tgattttgac gttgaatgct ccataagtta gcaacaacac
     1981 gcaacacagg caataagtta gcagctgtca caatggcaat atttttttca taagcttttt
     2041 caaaaagctc tggcgcgtgt tctgaaatca aaatataggc aggctcaatg ggcataaaca
     2101 aaaacacaaa atcgggcgta tttaatccct gtagtgtatg atattgacga ctacctaaat
     2161 cgttaatatg aaactctacc gctttaatat gtttgcttag ggcttcttga cgtgccttgt
     2221 cgttatcggc agccacatac tccatataag cgactaaaga cattttgcta tcaacaataa
     2281 tttgcttgcc ttctggcaat ttaaccacaa aatcaggacg ttgattttta ccctcatcat
     2341 ttttaaagtt ttcttctttg ctatattcaa tatttttacg caatcctgca cgctctaata
     2401 acaaatccac tttttgttcg ccccatgaac cttgaagct
//
LOCUS       DAOQMB010000356         2438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26911_length_2438_cov_14.851448, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000356 DAOQMB010000000
VERSION     DAOQMB010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26911_length_2438_cov_14.851448"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..542)
                     /locus_tag="PLY05_09900"
     CDS             complement(87..542)
                     /locus_tag="PLY05_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNC03651.1"
                     /translation="MQLNKFTDYGLRLLMYLIKSSDQLHTIAQVAHDLHISENHLVKV
                     SHFLAQQGWIISTRGKGGGIRIHPEAMYLSIGEVIRTLEHDEKVIDCLGIECNLRRDC
                     HLKSLFPLAIEQFYQYLNQYQLKDAWRALANEPSKLTQHNNLIQTINPS"
     gene            complement(651..1862)
                     /gene="hmpA"
                     /locus_tag="PLY05_09905"
     CDS             complement(651..1862)
                     /gene="hmpA"
                     /locus_tag="PLY05_09905"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008302999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HNC03652.1"
                     /translation="MTPEQIQLVQSTVPVLRENGVLLTKHFYQRMLGNHQNLKEVFNL
                     GHQRSGAQAQALANAVLAYAENIENPAVLMPVVELIANKHVSLNIQAPDYSIVGENLL
                     HSISEVLGIAMDHPLIEAWAIAYGQLADIFIQTERAIYQQHQQTKGSWLGWRAFKIAR
                     KTVESEEVTSFYVEPVDGGELPTYQAGQYISVRVFVEELGLKQPRQYTLSTHPQPNYL
                     RISVKREQGNNADQAAGWVSNTLHQLPEGAEIEVSAPTGNFFLINPNNKNVFISGGIG
                     LTPMIAMLNQLDSLGMPQPVHFFHACRNSEVHVMKKHIDQLKGQYPQFKTCIAYEFPH
                     EGNVQGQDYDIAGRLDLNHVDPSLLPKDADYYLCGPMPFMAEQQKTLIKLGINAAQIR
                     SEAFGTGGVSH"
     gene            2028..2231
                     /locus_tag="PLY05_09910"
     CDS             2028..2231
                     /locus_tag="PLY05_09910"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HNC03653.1"
                     /translation="MTINAGLRLVAGTFVIISVLLAHFVNPMWFLFTVFIGLNLIQSA
                     FTGWCPMVWLLGKLGFKREVVVN"
BASE COUNT          701 a          477 c          469 g          791 t
ORIGIN      
        1 tgctttctag cttaaaaaaa cgcatatatc ccgaaaattt gtgtacaaat gtatagatag
       61 tttttttgat cgttgatatg aataatttat gacggattaa ttgtttgaat gagattattg
      121 tgttgagtta gtttgctagg ctcatttgct aaggctcgcc acgcatcttt taattggtac
      181 tgattgagat actgataaaa ttgctcaatt gctaatggga acaatgattt aagatggcag
      241 tctcgacgta aattacattc tatgcccaaa cagtctatca ccttctcatc atgctccaag
      301 gttcgaataa cttcaccaat agataaatac atggcttcgg gatgaatacg aataccgcca
      361 cctttgcctc gtgtactaat aatccaacct tgctgcgcca aaaaatgact gactttcact
      421 aaatgatttt cagagatatg taaatcatgg gcaacttggg caatggtatg taattgatca
      481 ctggatttga ttaaatacat taataaacgt aagccgtagt cggtaaattt atttaattgc
      541 acaatttgct ctatctatca aaatcttgag atgacaatta tacccttctt ttatggtgtt
      601 ttgttgagtg tggctgttgg gattattcaa cctcaacagc ctttctttgg ttaatgggac
      661 acaccacctg taccaaatgc ttcactacgg atttgtgctg cattgatgcc caatttaatc
      721 aaggtttttt gttgctctgc cataaatggc atcgggccac acaaataata atccgcatct
      781 ttgggcaata aagatggatc aacatgattt aagtctaaac gccctgcaat atcatagtct
      841 tgtccctgca cattgccttc atgtggaaac tcataggcaa tacacgtttt gaattgtgga
      901 tattgtcctt ttaattgatc aatatgcttt ttcatgacat gaacttcgct attacgacag
      961 gcatgaaaaa aatgtactgg ttgaggcatt cctaaactgt ccaattgatt caacatagca
     1021 atcatgggag ttaagccaat accaccactg ataaaaacat tcttgttgtt tggattaatt
     1081 aaaaagaaat ttcctgttgg tgctgacact tcaatttctg cgccttctgg caattggtgc
     1141 aaggtattag aaacccaacc tgctgcttga tcggcattat tgccttgttc acgcttaacc
     1201 gaaattcgta aatagttcgg ttgtgggtgg gttgataaag tgtattgacg aggttgttta
     1261 agacccaatt cctcaacaaa gacacgcacc gaaatatatt gcccagcttg ataggtaggt
     1321 aattctccac catcaacagg ctcgacataa aaagaagtca cttcttcgct ttctactgtt
     1381 ttgcgagcga ttttaaatgc tctccagccc aaccaactgc ctttggtttg ttggtgctgt
     1441 tggtaaattg cacgttctgt ttgaataaaa atatcagcta attggccata agcgattgcc
     1501 catgcttcaa ttaatggatg atccatagca atgcctaaga cctcactaat tgaatgtaat
     1561 aaattttcgc caacaatact gtaatcaggt gcttgaatat ttaaactcac atgtttattg
     1621 gcaatcagct caacaacagg cattaaaacc gctggatttt ctatattttc agcatatgcc
     1681 aacacagcat tcgccaacgc ttgggcttgt gctccacttc gctggtgccc caaattaaat
     1741 acttctttta ggttctgatg attacccagc atacgctgat aaaaatgctt ggttagcaaa
     1801 accccatttt ctcgtaaaac gggcacagta gattgtacta attgaatttg ctctggtgtc
     1861 atggctcgcc cctttggtaa caatacaatt aaatataaga tgcatttaaa atacacctta
     1921 taaaatattt gtacaagtga attaattgat tcattcaatt gatgcttgcc aatataccat
     1981 agggggtata gtatattgac tatagacaaa taacagaggt aaagatcatg actattaatg
     2041 caggcttacg tttggtggca ggtacgtttg tgattatcag cgtgttatta gcgcactttg
     2101 ttaatccaat gtggtttttg tttaccgtat ttattggcct aaacttgatt caatctgcgt
     2161 ttacaggttg gtgtccgatg gtatggttat taggcaaatt gggctttaaa cgcgaagttg
     2221 tggttaattg agttattccc tcccctaaat taggggaggg ttagggtggg gttatgctca
     2281 tagaccccct ccctgcctcc ccctaaatta gggggaggag ttttctgata atctttagca
     2341 ttggaatcac gccatgaaaa ctgctttagc attagccagt ctcaccttag tcacttcggt
     2401 agcggctgcc cccttaaaaa cgaccacggt gagttatc
//
LOCUS       DAOQMB010000357         2437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_26932_length_2437_cov_13.288413, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000357 DAOQMB010000000
VERSION     DAOQMB010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26932_length_2437_cov_13.288413"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLY05_09915"
     CDS             complement(<1..381)
                     /locus_tag="PLY05_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC03654.1"
                     /translation="MLVKVPQQVKTLEKIPSFGCLDDSPRGRVLRAAAHLFRVNGYEG
                     TTVREIASLVGIQSGSLFHHFKSKEEILFTVMKEVIEYTSTKQNDAIGQVSSYREKLK
                     ALIISELYAINGVTCDAMTVLVFEW"
     gene            659..>2437
                     /locus_tag="PLY05_09920"
     CDS             659..>2437
                     /locus_tag="PLY05_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016143011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1446 domain-containing protein"
                     /protein_id="HNC03655.1"
                     /translation="MASKNSIRIGCASGFWGDTNTAAFQLVRQTQIDYLVFDYLAEIT
                     MSIMAGAKLKKPDAGYATDFVAHVMSRLLPEIAEKNIKVISNAGGVNPLACRDALEKL
                     IKEAGLNLKVAVVLGDDLVNQQQTYRDLNTKEMFSGEDMPPMLLSMNAYLGAIPIRDA
                     LAQGADIVITGRVVDSAVVLGPLLHEFNWSVDDYDKLAQGSLAGHIIECGAQCTGGNF
                     TDWQDVPHYDNMGFPVAECYADGAFVISKPEKTGGLISTATIREQLIYEIGDPQAYFL
                     PDVVCDFTQVQVEQVGDNLVKVSGAKGRAPSDSYKVSATYMDGYRLIVSFAMIGIDAV
                     AKGQRVAESIFSKVNKVLAKRGAPPLSSTHIEIVGSEQAYGANARPEAKQCREITVRM
                     VARYPVQEGLLFLASEIAQASTGMAPGLAGIMGGRPKPSPVIRLFSCLVPKASVPVSL
                     DISNERIAVTLPTEGGFVPAERLASGEVADNSQVSHKVPLVQLAWARSGDKGDSSNIG
                     VIARKPEYLPWIRASLTEEAVAAYCQHWLETRSEVIRYELPSLNALNFMLTYALGGGG
                     IASPRLDPQGKAHAQQLLAMPVKIPVE"
BASE COUNT          668 a          491 c          549 g          729 t
ORIGIN      
        1 ccattcaaat accaataccg tcatggcatc acaggtaaca ccattaatgg catataactc
       61 actaataatt aatgctttta atttttctcg ataactagaa acttggccaa ttgcgtcatt
      121 ttgtttggtc gaggtatatt caatgacctc tttcatgacg gtaaataata tctcttcttt
      181 gcttttaaaa tgatgaaata aactacccga ttgaatccca actaaagagg cgatttcgcg
      241 caccgttgtg ccttcatagc cgtttactcg aaacaaatgt gccgccgcac gcaacactcg
      301 gccacgagga gaatcatcta aacaaccaaa actgggaatc ttttctagcg tcttcacttg
      361 ttgcggtact tttaccaaca tggttgtctc tcttttaaaa acagtgtttt tgccacagaa
      421 attaagctgt aatttttaca agtcgttaca aatgtttgat gaggattcat ttgaatacac
      481 gtcaaagcat tggtaagact gtcgtcaacc gctaaatggc attaccaata caaaaattat
      541 cttgttatag attttacctg aatttgagta tgccgtgaaa ttgcctcttg cacaagcaag
      601 cacttgcttg gtatgttacg catattcgca aaaagatgca aagcatacgg gaaatgttat
      661 ggcaagtaaa aactctattc gaattggttg tgcatctggc ttttggggcg ataccaacac
      721 agcggcgttt caattagtgc gtcaaactca aattgattat ttggtatttg attatttggc
      781 tgaaattacc atgtcgatca tggcgggtgc caaattaaaa aaacctgatg caggttatgc
      841 caccgatttt gtcgctcatg tgatgtcacg tttgctgcca gaaattgccg aaaaaaatat
      901 taaagtgatt agtaatgctg gcggggttaa tcctctcgcc tgtcgtgatg ctttagaaaa
      961 attaattaaa gaggctggct taaatctcaa agtggctgtg gtcttgggtg atgatttagt
     1021 taatcaacaa caaacctatc gtgatttaaa taccaaagaa atgtttagtg gcgaagatat
     1081 gccaccgatg ttgttaagca tgaatgctta tttaggcgcg attcctattc gtgatgcctt
     1141 agcacaaggt gcagacattg tcattactgg ccgcgtggtt gattccgccg tggttttagg
     1201 cccattgttg catgagttta attggtcggt tgatgactac gacaaactcg cacaaggctc
     1261 tttggcaggt catattattg agtgtggcgc acaatgtaca ggtggtaatt ttaccgattg
     1321 gcaagatgtc ccccactacg acaatatggg cttccctgtc gctgagtgtt atgccgatgg
     1381 tgcatttgtt attagtaagc ccgaaaaaac aggcggttta attagcaccg ccaccattcg
     1441 tgaacaatta atttatgaaa ttggtgatcc acaagcttat tttttacctg atgtggtgtg
     1501 tgacttcact caagttcagg ttgagcaagt cggcgacaac ttagttaaag tcagtggtgc
     1561 aaaaggccgc gcgccgagtg atagctataa agtttcagcc acttatatgg atggctaccg
     1621 tttaattgtc agctttgcca tgattggcat tgatgcggtg gccaaaggtc aacgggttgc
     1681 tgagtccatt tttagcaaag tgaataaagt cttagctaaa cgtggtgcac caccgcttag
     1741 ctctacacat attgaaattg ttggcagtga acaggcatat ggtgcaaatg cacgaccaga
     1801 agccaaacaa tgccgcgaaa ttactgtgcg catggtggct cgttatcctg ttcaagaagg
     1861 tttattgttt ttggccagtg aaattgccca agcctcaaca ggcatggcac ccggtttagc
     1921 aggtattatg ggtggtcgtc caaagccctc tccagtgatt agattatttt cttgcttagt
     1981 tccaaaagct tctgtgcccg ttagtttaga tattagcaat gagcgtattg cggttacttt
     2041 acccacagaa ggtggttttg tgcctgctga gcgtttagcc agtggcgaag tggcagataa
     2101 cagccaagtg tcacataaag tgcctttagt gcaattagcg tgggcgcgta gtggtgataa
     2161 aggtgactcg tcaaatattg gtgtaattgc ccgtaagccc gaatatttac cgtggattcg
     2221 tgccagctta accgaagaag cagttgccgc ttattgtcaa cattggttag aaacccgtag
     2281 tgaagtaatt cgttatgagt tacctagctt aaatgccctg aattttatgc tgacttatgc
     2341 cttgggcggc ggcggcattg ccagtccacg tttagaccca caaggcaaag ctcatgccca
     2401 acaactgttg gctatgcctg tcaaaattcc tgtagaa
//
LOCUS       DAOQMB010000358         2431 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27066_length_2431_cov_5.013468, whole genome shotgun sequence.
ACCESSION   DAOQMB010000358 DAOQMB010000000
VERSION     DAOQMB010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27066_length_2431_cov_5.013468"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..>895)
                     /locus_tag="PLY05_09925"
     CDS             complement(153..>895)
                     /locus_tag="PLY05_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019384256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03656.1"
                     /translation="AWNAVSDFLGGSSSSDGRRDRDSYSSSSESYHTSKVTNYDPDKV
                     KVAELENERVGLVKEAQLELLEFNAKIEAAMIEARCRGFHAMQQAMMGMLKEVNVLAE
                     ERLMLLEKGSLQQIQQVEAMYADLKFDVQQDDFLQNKVPQLLNLAGQFPEGSEMRQTF
                     MKGIDQEMATHFSFKTNQLNKLNERCQIVVSSVISSKERMQAHIDTVITKRIEHIEQV
                     MQNNAQLEFKNPVLQLNSSATNETAKLD"
     assembly_gap    896..905
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1020..1430)
                     /locus_tag="PLY05_09930"
     CDS             complement(1020..1430)
                     /locus_tag="PLY05_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004681970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC03657.1"
                     /translation="MKKYMLDTNMIIYLIKQKPQSVMQHIMTFADDEQLVMSFVSYAE
                     LLKGVERSSKKQQALANLEQIIKQIQVVYPTDAKICHYYAQHFERLKQMGTPIGMNDL
                     WIACHALAEQAILVTHNIREFNRITDLSVENWVE"
     gene            complement(1427..1696)
                     /locus_tag="PLY05_09935"
     CDS             complement(1427..1696)
                     /locus_tag="PLY05_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNC03658.1"
                     /translation="MNQIVMPIIQQGNCQMVNIPNELRLDAERVEIYRNEAGDLVLHP
                     LAPNRGEALLQALSAFDDEFIDIMEDILAESHAENILNTSERDTL"
     gene            complement(1720..>2431)
                     /locus_tag="PLY05_09940"
     CDS             complement(1720..>2431)
                     /locus_tag="PLY05_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005251394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03659.1"
                     /translation="LEQSLDFVQEIVKSEQQTTESYLILVDKNQQDLLQQGINNSVEA
                     EQITAWFSEWRQERLLMLKQWQPLIEAGLQGVISEQTVLDTLQCMQHYQQQLDAFYLQ
                     KRLGIHTTYAFFPNGHRQEKLEKEQELIKLNHQFMQQLEKVIFSTSHTAQKIWLVRFS
                     EVWQQSMVQEIIGFLEKEQLIDRSDIVQIMSEELRQLQQQSFASVLQDAKDYSQALAQ
                     REKDTSTLIFKMRKALKT"
BASE COUNT          692 a          515 c          374 g          840 t
ORIGIN      
        1 tcacggactg gaatctaaat ccagcgcgtc taccaatttc gccactggcg caaggcggac
       61 tatcttacaa taaaaacaat aaaattcaaa gatttttgtt aatatgcaaa ttagttgctg
      121 ccaattaata ctcaacaatc attttgtata ttttaatcta atttagccgt ttcgtttgtt
      181 gccgatgaat tcagttgtaa aactggattt ttaaattcta attgagcatt attttgcatg
      241 acttgctcta tatgctcaat acgtttagta atgaccgtat caatatgcgc ttgcattcgc
      301 tctttactgc taatcaccga gctaacaaca atttggcaac gctcatttaa tttattcagt
      361 tgattggttt taaagctaaa gtgtgttgcc atctcttggt caatgccttt cataaaggtt
      421 tgacgcatct ctgaaccttc aggaaactga cctgctaaat ttaataattg tggcactttg
      481 ttttgtaaaa aatcatcttg ttgtacatca aattttaaat cggcatacat ggcttcaact
      541 tgttgaattt gttgcaaact acctttttct aacagcatca atctttcttc ggccaagaca
      601 ttcacttctt ttaacatgcc catcatcgct tgttgcatgg catgaaagcc tcggcaacgc
      661 gcctcaatca tagcggcctc aatcttggcg ttaaattcga gtaactctaa ttgtgcttct
      721 ttgactaaac ccactcgctc attttctagc tcagcgactt tgactttatc ggggtcataa
      781 ttggttactt tactggtgtg ataactttcg ctagaagatg aatagctgtc tctatctcgt
      841 ctgccatcgc tacttgatga accacccaaa aagtcactaa ccgcattcca agcatnnnnn
      901 nnnnnacctc tatcacttcc accatcatta ctagaaccgc cactaaaaaa attgcctatt
      961 gcactggccg cattgctaac aaaactacct aaaccactaa aaaatcccat gtttatctcc
     1021 tattcaaccc agttttcaac acttaaatct gtaatccgat taaattcgcg tatattatgt
     1081 gtcactaata tggcttgttc ggcaagcgca tgacacgcta tccataaatc gttcatgccg
     1141 attggtgttc ccatttgttt taatcgttca aaatgctgag cataataatg gcatattttg
     1201 gcatctgttg gataaaccac ctgtatttgt ttgataattt gctcaagatt agcaagtgcc
     1261 tgctgctttt tactactacg ctcgacacct tttaacaatt cggcataact cacaaacgac
     1321 atcactaatt gctcatcatc tgcgaaggtc ataatgtgct gcatcacact ctgaggcttt
     1381 tgcttaatca aataaataat catattagtg tcaagcatat actttttcat aatgtgtctc
     1441 gttcagacgt atttaatatg ttttctgcat gagactcagc caaaatatct tccataatgt
     1501 caataaattc atcatcaaaa gcagataatg cttgtaataa agcttcgcca cgatttggtg
     1561 ctaatggatg taacaccaag tcacctgcct cattacgata aatttctaca cgttctgcat
     1621 caagtcgtaa ttcattagga atgttgacca tttgacaatt accttgttga ataatcggca
     1681 tgacaatttg gttcattact tatctcctca ttacagtaat tacgttttta acgctttacg
     1741 cattttaaaa atcagtgtgc tagtatcttt ttctcgttga gctaaagctt ggctataatc
     1801 tttggcatct tgcaacacac tggcaaaact ttgctgttgt agttgacgta gttcttcact
     1861 cataatttgc acaatatcac tacggtcaat taattgctct ttttctaaaa agccaataat
     1921 ttcttgaacc atgctttgtt gccacacttc actaaaacgc actaaccaaa ttttttgtgc
     1981 ggtgtgactg gttgaaaaaa tcaccttttc taattgctgc ataaactgat gattgagctt
     2041 gattaactct tgttcttttt cgagtttttc ttgacgatga ccattaggaa aaaacgcata
     2101 agtggtatga atacctaaac gtttttgcaa ataaaaggca tctaattgtt gttgataatg
     2161 ttgcatacat tgcaacgtgt ccaacacggt ttgttcgcta atcacgcctt gtaaacctgc
     2221 ctctatcaat ggttgccact gttttaacat taacaagcgt tcttgtcgcc attcgctaaa
     2281 ccatgccgtt atttgttcag cttctacact attatttata ccttgctgca ataaatcttg
     2341 ttgattttta tcaactaaaa ttaaataact ttcggtggtt tgttgttcag atttgacaat
     2401 ttcttgcaca aaatctaagg attgctctaa c
//
LOCUS       DAOQMB010000359         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27163_length_2427_cov_3.234401, whole genome shotgun sequence.
ACCESSION   DAOQMB010000359 DAOQMB010000000
VERSION     DAOQMB010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27163_length_2427_cov_3.234401"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLY05_09945"
     CDS             complement(<1..553)
                     /locus_tag="PLY05_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03660.1"
                     /translation="MHKISLFLLALLTNISYADDCAKANELIQQTPQNSTAILNNYEQ
                     ALKLCTGQTGLYYAYGVALMQAEQYQAAYDAFKQALSLLNQNSQAPDFAKKRFSILLR
                     QSESQLAQYNKDANYNRGAVILSLSSVYAEAKASEFIIPKEFIALQERFQEQVNNEPL
                     SGQELKLALRSARDLAVEAPLAIE"
     gene            complement(566..2230)
                     /locus_tag="PLY05_09950"
     CDS             complement(566..2230)
                     /locus_tag="PLY05_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HNC03661.1"
                     /translation="MKKFYFYHLSSLVLGLSLTSNALANHIALLVGINDYPSPNQLEG
                     AVNDVQAIKKTLIDKWQFSEKDIVTLINKDATFNNIINELNRLKTRSKANDQVFIYFS
                     GHGTSSIDKGLKLALPSTSGAFIPVDFPLSGKHTLDSLQKTMIVGKWHLQPILKELEQ
                     DRSLFIAMDSCYSGNAVRALNQYPFHKRYMPVAFDDAFSDNLISTTNTKSTDNYPYKN
                     VVFLSAASDAEQADDISGDNLSFAPTLDGKPHGAMTDALLRVLNGTVAEADKNHDKQL
                     SYNEIHQAVRSLVQKNHNHTPMLLPSLPEDGQNITDHILFKQKDIILREPTSSTPKLQ
                     LKIDSPTPAVLEKLLNRPELNFSNTPDFIVSQTTKGLDLKKATGETILANSQLSDIKN
                     RLASQIWLQQHLARLSSTESLNLQTNKHERGNNFIANDTLAFMVKSSANAYLVLINIA
                     ANGEISVLYPSNTKGKEGQKIPAQQLVTIPSNDPQNWIRVVPPFGVDQVVAFALPQKP
                     RHWDELPNMVSLLPNTPQLVALDELLTSQKDIAWQQLELRSFPSAP"
     gene            complement(2243..>2427)
                     /locus_tag="PLY05_09955"
     CDS             complement(2243..>2427)
                     /locus_tag="PLY05_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03662.1"
                     /translation="SWGVNLQAAEPRGQSQVAFIVTPIDKTLEDVVQIDNGKLAAVAF
                     PTNSDWGIQRVYLEIK"
BASE COUNT          726 a          430 c          480 g          791 t
ORIGIN      
        1 attcgatggc caacggtgct tcaacagcta agtcacgtgc actacgtaag gctaatttca
       61 gttcttggcc tgataaaggt tcgttattga cttgttcctg aaagcgttct tgtaatgcaa
      121 taaactcctt gggaattata aattcagagg cttttgcttc agcataaaca ctagataaac
      181 tcaaaataac cgcaccacga ttatagttag catctttatt gtattgtgcc agttggcttt
      241 cgctttgacg taagagaata ctgaagcgtt tcttggcaaa atcaggagct tggctatttt
      301 gatttaataa cgataaagct tgtttaaagg catcatatgc ggcttgatat tgctcagcct
      361 gcatcaatgc aacgccataa gcataatata aacctgtttg acccgtacac agctttagag
      421 cttgctcata attgttcagt atagcagtag agttttgtgg cgtttgctgg atgagttcat
      481 tggctttggc acaatcatct gcataactga tattagttaa taacgctaat aggaaaagac
      541 tgattttatg cataaaaata accttttacg gagcagatgg aaaagagcgc aattcaagtt
      601 gttgccatgc tatatctttt tgactggtta acaattcatc aagtgcaaca agttgaggcg
      661 tattaggtaa taagctaacc atattgggta actcatccca atggcgtggt ttttgcggta
      721 aagcaaaagc aaccacttgg tcgacaccaa aaggaggaac aacacgaatc cagttttgag
      781 ggtcattact aggaatggtt actagttgtt gtgctggtat tttttgcccc tctttacctt
      841 tagtattact tggatataac acagaaattt caccgtttgc ggcaatatta attaatacta
      901 aataggcatt tgcggaactt ttaaccataa atgccaaggt gtcattggca ataaaattat
      961 tgccacgctc atgtttgttg gtttgtagat tgagagattc tgttgatgat aaacgagcta
     1021 aatgttgttg tagccaaatt tgacttgcca aacgattttt aatgtcacta agctgactat
     1081 tagccaaaat cgtctcacca gttgcttttt ttagatctaa acctttggtt gtctgactga
     1141 caataaaatc aggcgtatta ctgaagttta attcaggacg attcagcagc ttttctaaaa
     1201 ctgctggtgt agggctgtcg attttaagtt gtagttttgg agtagaagag gttggctctc
     1261 ttagaataat atctttttgt ttaaataaaa tatggtctgt gatgttttgt ccgtcttctg
     1321 ggaggctggg taataacata ggcgtatggt tatgattttt ttgtaccaaa ctacgtactg
     1381 cctgatgtat ttcgttataa cttaattgtt tatcatgatt tttatcagct tctgcaacag
     1441 tcccatttaa aactcttagc aaagcatccg tcattgcacc atgcggtttg ccatccaatg
     1501 ttggggcaaa actgagattg tcgccagaaa tatcatctgc ttgttcggca tcactggctg
     1561 ccgataaaaa aacaacattt ttatagggat agttgtcagt agatttggta ttagtggtgc
     1621 taatcaaatt gtcactaaat gcatcatcaa aagcgacagg catataacgt ttatgaaagg
     1681 gatattggtt taatgcacgt accgcatttc ctgaataaca gctatccatt gctataaaca
     1741 aggaacgatc ctgttctaac tctttaagaa tcggttgcaa atgccattta cccacaatca
     1801 tggttttttg taggctatct aaggtatgtt tgccagataa gggaaaatcg acgggaataa
     1861 atgctcctga ggtagaggga agtgccagtt ttagcccttt atctatggaa ctagttccat
     1921 gtcctgagaa ataaataaaa acttgatcat tagcttttga acgagtttta agtctattca
     1981 gctcattgat aatattatta aaagtggcat ctttattaat taaggtgaca atatcttttt
     2041 cagaaaactg ccatttatca ataagagtct ttttgatggc ttgaacatca ttaaccgcac
     2101 cttctaattg attagggctt gggtagtcat tgatgccaac taataacgca atatggttgg
     2161 ctaatgcgtt ggatgttagg ctaagaccta acacaagact tgataaatgg taaaaataaa
     2221 atttcttcat gatcatcgcc aattatttaa tctctagata aacgcgctga atgccccaat
     2281 cactattagt aggaaaggca acagcagcta atttaccatt atcaatttgc acaacatctt
     2341 ctagagtttt atcaattggc gtcacaataa aggcaacttg gctttgacca cgaggttctg
     2401 ctgcttgtaa attaacaccc catgagc
//
LOCUS       DAOQMB010000360         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27242_length_2422_cov_7.511618, whole genome shotgun sequence.
ACCESSION   DAOQMB010000360 DAOQMB010000000
VERSION     DAOQMB010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27242_length_2422_cov_7.511618"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..2384)
                     /locus_tag="PLY05_09960"
     CDS             complement(6..2384)
                     /locus_tag="PLY05_09960"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNC03663.1"
                     /translation="MAIKKSALYSSLWRGCDELRGGMDASQYKDYVLVLLFLKYVSDK
                     YADLDPDDADIVIPKGSSFADMVALKGDKEIGDKINKHIIAPIAEANNLKGIIDVTDF
                     NAEEKLGKDKEMVDRLTKLIAIFEGLDFSSNRAEGDDLLGDAYEYLMRHFATESGKSK
                     GQFYTPAEVSRVIAKIIGVNTATLASQTIYDPTCGSASLLLKAHDEAQVDISIYGQEM
                     DNATAALAKMNMILHGCSTADIVRGNTLSNPAFLDKHGQLKTFDFAVANPPFSTKAWR
                     NGFLPEEDSFNRFAYGIPPDKNGDYAFLLHILSSLNSTGKGAVILPHGVLFRGNAEAD
                     IRKTMITQGYIKGIIGLPANLFYGTGIPACIIVLDKENATARKGIFMVDASKGFSKDG
                     NKNRLRHQDIHKIVDVFTKQLELPKYSRLVAVSEIEANEYNLNIPRYIDSSEAEDLQN
                     IEAHLLGGIPHADIDDLARYWQVLPSLRSALFAPTTRPLFSQLTVAIANIKSTIFSHA
                     EFTAFNNNITALFNTWRSAHLPMLQGLTVGCHPKTLIQTLSESLLTTFKDAPLLDNYD
                     IYQHLMTYWDDVFQDDVYLISAEGWQAVLDNKPNEDLIPKPLIIARYFAAQQTNIEQL
                     EANRDAITRQMEELDEEHGGDDGLLADAKNDKGSVNKASLKMRLTAIKHDASAHEERQ
                     LLNQYAALIEQQATANKAIKDAQKALDVLVHKQYFDLDENAVKTLVINDKWLSRLAAD
                     VQSELDRVSQALTGRIKQLAERYETPLPLLSQELEGLSAKVDEHLKKMGFVW"
BASE COUNT          662 a          542 c          501 g          717 t
ORIGIN      
        1 cgcccctacc aaacaaaccc catcttcttt aaatgctcat ccaccttagc actcaaaccc
       61 tcaagctctt ggctcaacaa cggcaacggc gtttcataac gctctgccaa ctgcttaatg
      121 cggcctgtta aggcttggct gacacggtct agctcacttt gcacatctgc tgccaaacga
      181 cttaaccatt tatcgttaat gactaaggtt ttaaccgcgt tttcgtctaa gtcaaaatat
      241 tgcttatgca ccaacacatc caaggctttt tgtgcgtctt taatggcttt gttagcggtg
      301 gcctgttgct caattaatgc cgcatattgg tttaatagtt ggcgttcttc atgtgcgctg
      361 gcatcgtgtt taattgccgt taaacgcatt tttaacgagg ctttattcac cgaacctttg
      421 tcatttttag catcggctaa caaaccatca tcgccgccat gttcttcgtc taattcttcc
      481 atttgccgcg taatcgcatc ccgattggct tctaattgct cgatgttggt ttgttgtgca
      541 gcaaaataac gggcaataat taacggctta ggaatcaaat cttcattcgg cttgttatct
      601 aataccgctt gccaaccttc ggcactaatc aaatacacat catcctgaaa cacatcatcc
      661 caataagtca ttaaatgctg ataaatatca taattatcta acaaaggcgc atctttaaag
      721 gtagttaata agctttcgga cagtgtctga atcaaggttt ttggatgaca gcccaccgtt
      781 aaaccttgca acatgggcaa atgcgcgctg cgccatgtgt taaacaaggc ggtgatgttg
      841 ttgttaaacg cggtaaattc ggcatgacta aatatcgtgc ttttaatgtt ggcaatggcc
      901 acggttaatt gactaaacaa ggggcgagta gttggcgcaa acaaagccga acgtaaagag
      961 ggcaatactt gccaataacg agcaaggtcg tctatatcgg catggggaat accacccaac
     1021 aaatgcgctt cgatattttg caggtcttcg gcttcgctac tgtcgatata acgcggaatg
     1081 ttgaggttgt actcgttggc ttcaatctca ctcactgcca ctaaacgcga atacttgggc
     1141 agttcgagct gtttggtaaa cacatcaact attttatgaa tgtcttgatg acgtaaacgg
     1201 tttttgttgc cgtctttgct aaagcctttg ctggcatcga ccataaaaat gcctttgcga
     1261 gcagtggcgt tttctttatc caagacaata atacaagcag gaatgccagt gccataaaac
     1321 aagttagcag gcaagccaat aatgccttta atatagcctt gagtaatcat ggttttgcga
     1381 atatccgctt cggcattacc tctaaacaac acaccatgcg gcaaaatcac tgctcctttg
     1441 cctgtgctat ttaacgagct aagaatgtgc agcaaaaaag cataatcgcc atttttgtcg
     1501 ggtggaatac cataagcaaa gcggttaaaa ctgtcttctt cgggcaaaaa gccattacgc
     1561 catgcttttg ttgaaaatgg tggattagct accgcaaagt caaaggtttt aagttgaccg
     1621 tgtttgtcca aaaaagcagg gttagataag gtattgccac gcacaatgtc cgctgttgag
     1681 caaccatgca aaatcatgtt cattttggca agggcggcgg tggcgttgtc catttcttga
     1741 ccataaatag aaatatcaac ctgtgcttcg tcgtgggctt ttaacaacag cgaggcagag
     1801 ccacacgttg ggtcataaat ggtttgactg gcgagggtgg cggtattaac gccaataatt
     1861 ttggcaataa cccgtgacac ttcggctggg gtataaaatt ggcctttgct tttgcccgat
     1921 tcggtagcaa agtggcgcat caagtattcg taggcatcac ccagtaagtc atcaccttcg
     1981 gcgcggttgc tactaaaatc taagccttca aagatagcga taagcttggt tagacggtca
     2041 accatttctt tatctttgcc gagtttttct tcggcattaa agtcagtaac gtcaataatc
     2101 cctttaaggt tatttgcttc ggcaatgggg gcaataatat gtttattgat tttgtcgcca
     2161 atttctttgt cgccttttag cgcaaccata tcggcaaaac tactaccttt gggaataaca
     2221 atatcggcat catctgggtc taaatcggca tatttatcag agacgtattt taaaaataac
     2281 aacaccaata cataatcttt gtattgcgag gcatccatgc cgccgcgtag ttcgtcacat
     2341 cctctccata aagagctgta cagtgctgat tttttaatgg ccatttgtgc tatccgttaa
     2401 tgcaaatttt taaaaaatac ta
//
LOCUS       DAOQMB010000361         2417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27337_length_2417_cov_4.061812, whole genome shotgun sequence.
ACCESSION   DAOQMB010000361 DAOQMB010000000
VERSION     DAOQMB010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27337_length_2417_cov_4.061812"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1154
                     /gene="cas1"
                     /locus_tag="PLY05_09965"
     CDS             168..1154
                     /gene="cas1"
                     /locus_tag="PLY05_09965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNC03664.1"
                     /translation="MESIYIDRRDTTLYVEGARLLVHHPDMVKAQSIPLSHLRFVVIS
                     ANVSLTTNVLQAFSKAEIACIVINPRKTDDMTFTMPFRHGHAERRLAQYDLYHRADLR
                     QQAAQNLVLFKITTQQKTLQRLLINHPQHKRGLTKSIESLQGIRQILKEQAISIERLR
                     GFEGAAASNYFTGLSYCVAASLYFTGRNRRPPKDPVNAILSLTYSLIHYESIRCLTAV
                     GLDPMIGFYHDLSYKRESLACDMVELLRARIDYWVIQQFNQQVLRVDHFSMDESVNGG
                     CTLGKAGRACFYAAYEYQAKHWRKVLRQIARNWLKQLKPYFMTLTDEDNNHE"
     gene            1147..1920
                     /locus_tag="PLY05_09970"
     CDS             1147..1920
                     /locus_tag="PLY05_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03665.1"
                     /translation="MSDYQSLVLKSAALRRLWLQDNGLRIMPKFSSERRVPLCRLLRV
                     ECYGDIGQVKDGFCTLLKIAEQHIPVTFFDSSNHVCAQLYHPCPKTKPLALWLEHCFC
                     EQACQEDYKNWLNNQHYALLAHLHITQGSLTTRQQLLQQQLIVYLKSRQLDSVFTELY
                     QQGKRMLTVVLGEQLMAHGLAAGTSLRNKLFSDLHDLMEYWIQGDILQWLQRFSNVND
                     HLKRDFYSHQYNALSEKITLILTQLQEMLEQYALEQLHN"
     gene            1942..>2417
                     /locus_tag="PLY05_09975"
     CDS             1942..>2417
                     /locus_tag="PLY05_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03666.1"
                     /translation="MKIIFSHIPFIISADTEKLSRVERAGYYRNLGITQEQFEQRQTS
                     IKARHDQTLNEMLNNTAPKITLAHTDSEHKLLSQEQIELYYEFGIEPPIEKLNFTQEF
                     DREIYAIYGIEPFLPSTLKSTFYFVMSEGGLSIKEHGAINDALDEFVSKIAIKGLR"
BASE COUNT          738 a          496 c          458 g          725 t
ORIGIN      
        1 accgttaccg aacgtcagcg tttactcaag cgtgttttag gcgtgattga tgaaaccgaa
       61 gacttctttt tgattgtgcc acttcgccaa agtacgcctg ttaaagccat tggcaccgcc
      121 actccccctg cggatgacaa ctttttatat attggttaga ggtttttatg gaaagcattt
      181 atatagaccg tcgtgatacc accttgtatg tcgaaggcgc acgcttgctg gtgcatcatc
      241 ctgatatggt taaggcacaa tctattcctt tatctcattt acgatttgta gtcatttcgg
      301 ccaatgtgtc tttaaccacc aatgttttac aagcctttag caaagccgaa attgcctgta
      361 ttgtcattaa tccacgcaaa accgatgaca tgacctttac catgccgttt agacatggcc
      421 acgccgaacg ccgtttagcc caatatgact tatatcatcg tgccgattta cgccaacaag
      481 ctgctcaaaa cctcgtactc tttaaaatca ccacccaaca aaaaacctta caacgtctat
      541 taattaatca tccccagcat aaacgcggtt taactaaatc cattgaaagt ttacaaggca
      601 ttcgtcaaat cttaaaagaa caagccatca gtattgagcg tttacgaggt tttgaaggtg
      661 cggccgccag taattatttt acaggactca gttattgtgt cgccgccagt ttgtacttta
      721 cgggtcgcaa tcgccgtcca cccaaagacc ctgtcaatgc tattttgtct ttaacctaca
      781 gcttaattca ttatgaaagt attcgttgct taacagcggt cggcttagac ccaatgattg
      841 gtttttatca tgacctaagt tacaaacgcg aatccttagc ctgcgacatg gtagagctat
      901 tacgcgctcg tattgactat tgggtgattc aacagtttaa ccaacaagta ttaagagttg
      961 accattttag tatggatgag agcgtcaatg gtggttgcac actgggcaaa gcaggacgcg
     1021 cttgttttta tgctgcctat gaatatcagg ccaaacactg gcgcaaagtc ttaaggcaaa
     1081 ttgctcgcaa ctggcttaaa caacttaagc cctattttat gactttgact gatgaggata
     1141 acaaccatga gtgattatca aagtttagtc ttaaaatctg ctgccttacg gcgtttgtgg
     1201 ttacaagata atggcttgcg cattatgccc aaatttagca gcgaacgacg tgtgcctttg
     1261 tgtcgcttgt tgcgtgtgga gtgttatggc gatataggtc aagttaaaga tggattttgt
     1321 accctactca aaattgccga acagcatatt cctgtcactt tttttgatag tagcaatcac
     1381 gtatgtgctc agctttatca tccctgcccc aaaaccaaac cattagcttt gtggttggaa
     1441 cattgttttt gtgagcaagc ctgccaagaa gattataaaa actggttaaa caatcaacat
     1501 tatgcattat tggcacattt gcatattaca caaggtagct taacaacacg tcagcaatta
     1561 ttgcaacaac aactgattgt ctatcttaaa tctcgtcaat tagacagtgt atttaccgag
     1621 ctatatcaac aaggcaaacg tatgttaacg gtggtattag gtgaacaatt aatggcacat
     1681 ggtttggcgg ctggcacaag cttgcgaaat aagctcttta gcgacttaca tgatttaatg
     1741 gaatactgga tacaaggtga tattttgcaa tggctacaac gcttttctaa tgttaatgat
     1801 catttaaaac gtgattttta tagccatcaa tacaacgctc tttctgaaaa aataacactg
     1861 atactcactc aactacaaga aatgttagaa caatacgctt tagagcaatt acataactag
     1921 tttttaaatg aggtaaccat tatgaaaatt atatttagtc atataccttt tattataagt
     1981 gctgatacag aaaaacttag tcgtgttgaa cgtgcaggtt attaccgaaa tcttggcatt
     2041 acacaagaac agtttgaaca acgacaaaca agtattaaag cacgtcatga ccagacattg
     2101 aatgagatgc ttaacaatac agccccaaaa ataacccttg cacatacaga cagcgagcat
     2161 aaattactct cgcaagagca gatagagctt tattatgagt ttggcataga gccacctatc
     2221 gaaaaactca attttacaca agaatttgat cgagaaattt atgctatata cggcatagag
     2281 ccatttttac cgtcaacatt aaagtcaact ttttattttg taatgtccga aggtggctta
     2341 agcattaaag agcatggtgc tataaacgat gctttagatg agtttgtctc taaaattgcg
     2401 attaaaggat tgcgcaa
//
LOCUS       DAOQMB010000362         2412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27450_length_2412_cov_3.447179, whole genome shotgun sequence.
ACCESSION   DAOQMB010000362 DAOQMB010000000
VERSION     DAOQMB010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27450_length_2412_cov_3.447179"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1807)
                     /locus_tag="PLY05_09980"
     CDS             complement(<1..1807)
                     /locus_tag="PLY05_09980"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004669060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HNC03667.1"
                     /translation="MNSIDQIERITQNRVVNLFKQQLGYHYLGDWQDRANNRNIEVPY
                     LSAFLQQQGHSQALINKAIYELQKTASDQTLNLFYRNKTVYQLLRYGLKIKADIGENT
                     QTIWLIDWKNHHNNHFAFAQEVTIKGANAKANNKRPDIVLYVNGIALGVIELKRSTVS
                     VSEGIRQNLDNQKPQFIEPFFSTQQLVMAGNDSQGIRYGTTLTAEKYYLTWKEPFANN
                     SLSLLDVHLCQLCEKTRFLELIHDFVVFDFGTKKLCRPNQYFGIKAAQAYIKRREGGI
                     IWHTQGSGKSLTMVWLTKWIRENIPDARVLLITDRTELDEQIEKVFMGVNEKIYRTKS
                     GADLIAQLNVTTEWLLCSLIHKFGNKEEGDIQHYVDEVKKFLPLNFKAKGDIYVFVDE
                     CHRTQSGALHEAMKSILPHAVFIGFTGTPLLKADKQKSIEIFGSYIHTYKFNEAVKDG
                     VVLDLRYEARDIDQYLTSPKKIDEWFESNTKDLTDIAKAQLKARWGTLQKVLSSKDRL
                     QMIVNDIMMDMAKKPRLMDGRGNAMLVSSSIYQACKFYELFQQTPLQGKCAIVTSYKP
                     TTTDIKGEETGHGLTEKLHQYDIYQKMLNGKDANKF"
     gene            complement(1910..>2412)
                     /locus_tag="PLY05_09985"
     CDS             complement(1910..>2412)
                     /locus_tag="PLY05_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03668.1"
                     /translation="QGYNYAQAGAYFVTICSQQRAHLFGEIAEGKMLLNECGHIVEQV
                     WHELPQHYANISLAEFVIMPNHIHGVIILTENISVRAGVGVGFVRAGFKPAPTQHGLS
                     EIIRGFKTFSARRINQYRNSIGVPVWQRNYYEHIIRNDMDYQRIAEYIINNPQRWTED
                     SLNVHM"
BASE COUNT          605 a          492 c          535 g          780 t
ORIGIN      
        1 caaatttatt ggcatctttg ccgttgagca ttttttgata aatgtcgtat tgatgcagct
       61 tttcggttaa gccatgacct gtttcttcgc ctttaatatc ggtggtggtg ggtttgtaag
      121 aggtgacaat cgcgcatttg ccttgtaacg gtgtttgttg aaatagctca taaaacttac
      181 aggcttgata aatactgcta gataccagca tggcattgcc acgaccatcc attaagcgcg
      241 gttttttggc catatccatc atgatgtcgt tgacaatcat ttgtaggcgg tctttggagc
      301 taaggacttt ttgcagtgtt ccccaacggg ctttaagttg cgctttggca atgtcggtta
      361 agtctttggt attggattca aaccattcat ctattttttt gggagaggtg agatattggt
      421 caatatcacg cgcttcgtag cgtaaatcta acaccacgcc gtctttaacc gcctcattaa
      481 atttgtaggt gtgaatataa ctgccaaaaa tttcaatgct tttttgtttg tcagctttga
      541 gtaggggcgt acctgtaaaa ccaataaaca cagcatgagg caaaatgctt ttcatggctt
      601 catgcaatgc tccagattgc gtgcggtgac attcatccac aaaaacataa atatcgcctt
      661 tggccttaaa gttaagcggt aggaattttt taacctcatc gacatagtgc tgaatatcgc
      721 cttcttcttt gttgccaaat ttatgaataa gcgaacacaa cagccattct gtggtgacat
      781 tcagttgagc aattaaatcc gcgccgcttt tagtgcgata aatcttttca ttgactccca
      841 taaacacttt ttcgatttgt tcatcgagtt cggtgcggtc ggtaatgagc aacacacgcg
      901 catcggggat attttcgcgt atccatttgg tcagccaaac catggttaaa ctcttgccac
      961 tgccttgtgt gtgccaaata atgccacctt cacggcgttt tatataagct tgagcagctt
     1021 taatgccaaa gtattgatta ggccgacata gttttttagt gccaaaatca aacaccacaa
     1081 agtcatgaat caactctaaa aagcgcgttt tttcgcacag ttggcacagg tgaacgtcta
     1141 ataggcttag gctgttattg gcaaacggtt ctttccaagt gaggtaatat ttttcggcgg
     1201 tgagagttgt gccataacgt atgccttggc tgtcattacc tgccatgact aattgttgtg
     1261 tgctaaaaaa tggctcaata aactgcggtt tttgattgtc taaattttgc cgtatgcctt
     1321 cactcaccga caccgttgag cgtttgagtt caatcacccc caaagcgata ccattaacat
     1381 atagcacaat gtcggggcgt ttgttgttgg ctttagcgtt cgcgccttta atagtcactt
     1441 cttgggcaaa agcaaaatga ttattgtgat ggtttttcca gtcaatcagc cagatggttt
     1501 gggtgttttc gccaatatcg gctttaattt ttaggccata acggagcaac tgataaaccg
     1561 ttttgttgcg ataaaacagg tttaacgttt ggtcgctggc ggttttttgt aactcataaa
     1621 tggctttatt gataagtgct tgactatggc cttgttgttg caaaaacgcg cttaggtatg
     1681 gcacttcgat gttgcgatta ttggcgcggt cttgccaatc gcctaaataa tggtagccga
     1741 gttgttgctt aaacagatta acaactcggt tttgagtgat gcgttcgatt tggtcgattg
     1801 agttcatggt ttttatgtct tttataaatt gatattgtag gggcgggttt ccgtaagggc
     1861 gggtttccgt aagggcgggt ttccgtaagg gcgggtttca aacccgcccc tacatatgaa
     1921 catttaatga gtcttccgtc catctttgcg gattattgat aatatattca gcaattcgtt
     1981 ggtagtccat gtcgttgcga ataatatgct cgtaatagtt acgctgccat acaggtacac
     2041 ctatcgagtt gcgatattgg ttgatacgcc gcgccgaaaa tgttttaaag ccgcgtataa
     2101 tttcggatag cccatgttgt gtaggggcgg gtttaaaacc cgcccttacg aaacccaccc
     2161 ctacacccgc ccgtacagat atattttcgg ttaatataat aacgccatgt atatgattgg
     2221 gcataatcac aaattcagcc agtgagatat tggcatagtg ttgtggcagt tcgtgccaaa
     2281 cttgctcaac gatatggccg cactcattta gcagcatttt tccttcggca atttcaccaa
     2341 ataaatgcgc cctttgttgg ctgcaaatgg tgacaaaata cgcacctgct tgtgcgtaat
     2401 tatatccttg ta
//
LOCUS       DAOQMB010000363         2410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27487_length_2410_cov_4.418259, whole genome shotgun sequence.
ACCESSION   DAOQMB010000363 DAOQMB010000000
VERSION     DAOQMB010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27487_length_2410_cov_4.418259"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1905
                     /locus_tag="PLY05_09990"
     CDS             49..1905
                     /locus_tag="PLY05_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HNC03669.1"
                     /translation="MTKPCYWQHCAAAFKEQGQALSSSPVANNSNKAWIVNFNNGNDN
                     NNNKNNNNYVRLVRSSECLPVLVLPSVCFYHQYNVRLPLFQRGIEGDLEILPNPPLAK
                     EGTEISPSPSLQKRGTSSQKTITHKEQGLKPLYQAFTQAKKRKKPSYNRLMFEQDWLS
                     HLFTLLNALQRHTWRPLPSVCFVAKRPKAREIHAPDFADRVVHHWLVPQLEAIYEPVF
                     IYDSFANRKHKGTHAAVSRLQDFVRQVHSGQGGGYYLQLDIHNFFNSIHRPTLYGLLK
                     AKIHPNPPLPKEGDSSLKRVQSVSPFVKGGLRGICNQQISPNPSLQKRGTIQCKSTKR
                     LGTQEHLLRTIHALLRQHPKHLASHSRATPQELALVPAHKQLKNAAKGCGLPIGNLSS
                     QFFANVYLNELDQFVKHTLKAKRYVRYVDDFVLVHHNKAQLEAWLEQIEQFLAQRLQL
                     KLKNDIKLQPLTSGIDFLGYWIYPTHKLVRPRVIHHAKQKLSAWQSAYVRRTAQGWLI
                     NATQQEQAAIRSVWASYQGHFGHANTVKLEQFFYKRYGWLQQIMPYVAPIPTRKIHPS
                     PPLQNKTKSPLTPLYQRGGQGGASIQSVSPFVKGGLRGIHSPPFYIKAKETP"
     gene            1902..2405
                     /locus_tag="PLY05_09995"
     CDS             1902..2405
                     /locus_tag="PLY05_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC03670.1"
                     /translation="MNKPNLFSVIALLLAITHSHAQTCNPNITRSAPDSRYELLNNGT
                     EVKDSQTGLIWQRCSLGQTWSGTSCTGTATTYNWTNALQTAANMGNGWRVPNIKELDS
                     LVEQACYNASINETYFPNTASNWYWSSSPVAHYSNAAWIVSFSNGYADYGSKYANLYV
                     RLVRSSQ"
BASE COUNT          697 a          620 c          527 g          566 t
ORIGIN      
        1 ccctctccct agtggagagg gctgggggga gggtacagag gtgcaaccat gaccaagccg
       61 tgctactggc aacactgtgc ggctgcgttt aaagagcagg ggcaagcgtt gtcgtcctcg
      121 cctgtggcta acaatagtaa caaagcgtgg attgtcaact tcaacaacgg caacgacaac
      181 aacaacaata agaacaataa caactatgtg cggttagttc gttccagtga gtgtttacct
      241 gtgcttgtgc tgccgtctgt ttgtttttat catcaatata atgtgcgcct cccccttttt
      301 caaaggggga ttgaggggga tttagaaatc ctccctaacc ctcctttggc aaaggagggg
      361 acagaaatct cccctagtcc ctctttacaa aagaggggaa cttcatcgca aaaaacaata
      421 actcataaag agcaaggtct taaaccccta tatcaagcct ttacccaagc caaaaagcgc
      481 aaaaaaccaa gctataatcg tttaatgttt gagcaagact ggttaagcca tttatttacc
      541 ctcttaaacg ccttacaacg tcacacatgg cgacccttgc ccagtgtgtg ctttgtggcc
      601 aaacgcccca aagcccgcga aatacatgcg ccagactttg ctgacagggt agtgcatcat
      661 tggctggtgc cgcagttaga agccatttac gaacccgtgt ttatttacga cagctttgcc
      721 aaccgcaaac acaaaggcac tcatgcggca gtcagccgtc tacaagactt tgtgcgccaa
      781 gtacacagtg gacaaggcgg tggttattat ttgcagttag atattcacaa tttttttaat
      841 agcattcatc ggcctacttt gtatggtttg ttaaaagcta aaatccaccc caaccctcct
      901 ttaccaaagg agggggattc gtcgctaaaa agagtgcaga gcgtctcccc ctttgtcaaa
      961 gggggactaa gggggatttg taatcaacaa atctccccta acccctcttt gcaaaagagg
     1021 ggaacaatac aatgcaagag tacaaagcgt ctggggactc aggagcattt attacgcacc
     1081 atacacgcct tattgcgtca acaccccaaa caccttgctt cgcatagtcg cgccacacca
     1141 caagaattag cccttgtgcc agcgcacaaa caactcaaaa atgccgccaa aggctgtggc
     1201 ttgcccattg gcaatttaag cagccagttt tttgccaatg tttaccttaa cgaactcgac
     1261 caatttgtaa aacacacttt aaaggccaaa cgctatgtgc gttatgtaga tgactttgtg
     1321 ctagtgcatc acaacaaagc ccaattggag gcatggcttg agcaaataga gcagttttta
     1381 gcccagcgtt tgcagctaaa gctcaaaaac gacatcaaat tgcagccttt aacctcaggt
     1441 atcgactttt tggggtattg gatttaccca acgcacaagc tagtaaggcc acgcgtgata
     1501 catcatgcca aacaaaaact cagtgcatgg caaagtgcct atgtgcgtcg caccgcacaa
     1561 ggttggctta ttaacgccac ccagcaagag caagccgcta tacgcagtgt atgggcaagt
     1621 taccaaggcc attttggcca tgccaatacc gttaagttag agcagttttt ttacaaacgc
     1681 tatggttggt tgcagcaaat aatgccttat gttgcgccaa tacccacacg caaaatccac
     1741 cccagccctc ctttacaaaa caagacaaaa tctcccctaa cccctcttta tcaaagaggg
     1801 ggacagggcg gtgcaagcat acaaagcgtc tccccctttg ttaaaggggg actaaggggg
     1861 attcattcgc caccttttta catcaaagcc aaggaaacac catgaacaaa cctaatcttt
     1921 ttagcgtaat tgccttgcta ttggccatca cccatagcca tgcccaaacc tgcaacccca
     1981 acataacccg tagcgcacct gacagccgtt atgagctact taataacggc acagaggtta
     2041 aagactcaca aacaggctta atatggcagc gttgcagctt aggccaaacg tggagcggca
     2101 caagctgcac aggcacagcc acaacgtata attggacaaa tgccctgcaa acggcagcca
     2161 atatgggtaa tggctggcgc gtgccaaaca ttaaagagct agatagctta gtcgagcaag
     2221 cgtgttacaa cgcgagtatt aacgagactt actttcctaa taccgccagc aattggtatt
     2281 ggtcgtcctc gcctgtggct cactatagta acgccgcgtg gattgtcagc ttcagcaacg
     2341 gctacgccga ctacggcagt aagtacgcta acctctatgt gcggttagtt cgttccagtc
     2401 agtaaattgg
//
LOCUS       DAOQMB010000364         2395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_27785_length_2395_cov_10.280769, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000364 DAOQMB010000000
VERSION     DAOQMB010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27785_length_2395_cov_10.280769"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2360
                     /locus_tag="PLY05_10000"
     CDS             <1..2360
                     /locus_tag="PLY05_10000"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03671.1"
                     /translation="NDFLGQDRAKEAVHMALAMPHDGYNIFAIGENGLGKRTMIKRLL
                     IDVAQHEEAPSDWCYVNNFSDPRTPIALELPAGKAPAVKKSLTKLWRSISRAVQASFQ
                     HETYVGRVEMLKGELTQAQQTALQELAQEGEKRHLKLLLRTPGGHGFSPIGEDGEVMS
                     VEAFNALTPEEQQQRKDAMQEMEKRLQKLADRLGRLEDQSRDQVQKLNDEVTLLAVEP
                     LIKKVKEQYEDLKPVVDYLSAYQQDIVDNVDLIVNNQEMETDVVANVSSDNAIPSRYQ
                     INVVVTHNPKKGAPVVFEDLPTHYNLMGHVEQVTYMGTVATDFTLIRGGALHRANGGY
                     LLLEAEQVLEQPYAWQGLKRALRSRSLKLSSLEQMLTLTGTISLEPDAIPLDVKIVLL
                     GDRETFHLLQEYDPELEQLFKIRADFANSMSRTIEHEQKYAHFLADCVAKEKLMPFDK
                     SALMVLIEESARNAEDQHKLSLHAASVGDLLREANYWALQQQQKLVSAEHVRLALAGQ
                     ERRRGQLRELYLEDIAQGTQLILCDGAVVGQVNALTVIHYADSEFGLPSRITATVHQG
                     GGDVLDIERTVDLGGSLHAKGVLLLSSYLKSKFGRDKPLHFSASIAMEQNYGGVEGDS
                     ATMAELAALVSAITELPIRQDLGITGSMNQMGEIQPIGGVNAKIEGFFAACQLKGMTG
                     NQGVIIPEQNVRNLMLRPEVLQAVEQGQFSIYAVSHAYQALELLLSRAMGEMNQKGKY
                     PTDSIMALVLAQLKHWHDIEKGDKPKKKKVKKAKKMKEANLPNGG"
BASE COUNT          730 a          437 c          563 g          665 t
ORIGIN      
        1 ttaatgattt tttaggccaa gatcgtgcca aagaagcagt acacatggct ttagctatgc
       61 cgcacgatgg ttataatatt tttgcgattg gcgaaaatgg cttgggcaaa cgtaccatga
      121 ttaaacgttt gttgattgat gttgctcagc acgaagaagc accaagtgat tggtgttatg
      181 tcaataactt tagcgatcca cgcacgccca tcgctctaga attaccagca ggaaaagcac
      241 ctgccgtaaa gaaaagttta accaaattat ggcgcagtat tagccgtgca gtacaagcca
      301 gttttcaaca tgaaacttat gttggtcgtg ttgaaatgct caaaggagag ctgacacaag
      361 cacagcaaac cgctttgcaa gagttggcac aagaaggtga aaagcgccat cttaaattat
      421 tgttacgcac accgggtgga catggttttt cgccgattgg tgaagatggc gaggtgatga
      481 gtgttgaagc atttaatgcc ttaacgcctg aagagcaaca gcaacgcaaa gatgccatgc
      541 aagaaatgga aaaacgcttg caaaaattgg cggatcgttt ggggcgttta gaagatcaaa
      601 gccgtgatca agtacaaaag ctgaatgacg aagtgacatt attggcagtt gagccattaa
      661 tcaaaaaagt caaagagcaa tatgaagact taaagcctgt ggtcgattat ttgtcggctt
      721 atcaacaaga tattgtcgat aacgtagatt tgattgtcaa caatcaagaa atggaaaccg
      781 atgttgtggc taatgttagc agtgacaacg ccattccttc acgttatcaa attaacgttg
      841 ttgttactca caatcccaaa aaaggcgcgc ctgttgtttt tgaagattta cccacgcact
      901 acaacttaat gggacacgtt gaacaagtca cttatatggg cacagtggct accgatttta
      961 ccttgattcg tggtggggca ttacatcgtg cgaatggcgg ttatttgttg ctagaagcgg
     1021 agcaggtgtt agaacagcct tatgcgtggc aagggctaaa acgtgcttta cgttcacgtt
     1081 ctttaaaatt gtcgtcttta gagcaaatgt taaccttaac agggactatt tccttagaac
     1141 cagatgctat tcccttagac gtaaaaattg tgttattggg tgatcgagaa acttttcatt
     1201 tattacaaga gtacgatcct gaattagaac aattatttaa aattcgtgcc gattttgcca
     1261 atagtatgtc acgtaccatt gagcatgaac aaaaatatgc tcatttttta gcggactgtg
     1321 ttgctaaaga aaaattaatg ccatttgata aaagtgcatt gatggtgtta atcgaagaaa
     1381 gtgcgcgtaa tgctgaagat caacacaaat tatctttaca cgctgccagt gtcggcgatt
     1441 tattgcgtga agccaattat tgggcgttac aacagcaaca aaaattagtc tctgccgaac
     1501 atgtgcgttt agccttagca ggtcaagaac gtcgtcgtgg tcaattacgt gagttgtact
     1561 tagaagatat tgcccaaggc acacagttaa ttttgtgtga tggtgcagtg gttggtcaag
     1621 ttaatgcctt aacggtcatt cattatgctg acagcgaatt tggtttaccg tctcgtatta
     1681 ccgcaacagt gcatcaaggt ggcggtgatg tcttagacat tgagcgtacc gttgatttag
     1741 gcggctcttt gcacgccaaa ggggtattgc tgttatccag ttatttaaag tctaaatttg
     1801 gccgtgataa acccttacat ttctcagcca gtattgctat ggagcaaaac tatgggggcg
     1861 tggaaggtga tagcgcaacc atggccgaat tagcagcctt agtttctgcc attactgagc
     1921 tgccaattcg tcaagattta ggcattactg gttcgatgaa ccaaatgggt gaaatccaac
     1981 cgattggtgg ggtaaatgcc aaaattgaag gcttttttgc tgcttgccaa ttaaaaggca
     2041 tgacaggcaa tcaaggggtg attattcctg aacaaaatgt gagaaacctc atgttgcgcc
     2101 cagaagtttt gcaagcggtt gaacaaggtc aatttagtat ttatgcggtg agtcatgcct
     2161 atcaagcttt agagttgttg ttatcacgcg ctatggggga gatgaatcaa aaaggtaaat
     2221 atccgaccga cagtattatg gctttagtgt tggcacagct caaacattgg catgacattg
     2281 aaaaaggcga taaacccaaa aagaaaaagg tgaaaaaagc taagaaaatg aaagaagcca
     2341 acttgcccaa tggcggttaa tttgttgata ctaaatcaac aatgcttgtt agcag
//
LOCUS       DAOQMB010000365         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_28064_length_2382_cov_11.598195, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000365 DAOQMB010000000
VERSION     DAOQMB010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28064_length_2382_cov_11.598195"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /locus_tag="PLY05_10005"
     CDS             <1..1965
                     /locus_tag="PLY05_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC03672.1"
                     /translation="AMGANDISQVLETVPDLHVGISDQVFAPKYNIRGITSNRNAQTL
                     MLINGIPITNLFTGNRGQIWMGMPVKGVARIEIIRGPGSALYGADAFAGVINIITKNA
                     DDIYGTKLGAGVGNFGQRSAWMQHGSTHNSYKTSFNLEAYKTEGWDSIIKSDAQTLND
                     QAFQTNASLAPNKVNTMKEVLEARFELTGLNDILRIGYQGNYNIGTGAGLIQALDPEG
                     RFKAERFNVDYAYNFNELVENWQIQTRTSFYRSTQQVEKNLLLFPRNFALSFDGNKTF
                     FKYPDGFIGNPSLKEDHARFDVNGLFKGLNKHLIRVGTGFYWGDIFEVTESKNFFSNY
                     TPRPSLTDVSDTNDAFLPEKERTNYYAFGQDEWQIANTLQLTTGLRYDHFSDFGSTIN
                     PRLALIWANTPDITTKLLYGRAFRAPSISELFVDNNPVQNGNPNLKPEIIDTYELALS
                     HQVSDNLLYNTNIYYYKIKDVCLLTDFVHKGEYNTNIYYYKIKDYITLVPINAFANQM
                     QNSGKRHGHGLSAEVDYSALDNLRLVANYSYQLSKDDATKTASGDTPNHQLYLRGEWE
                     ITPAWLLSPQVNVIGQQKRAAGDIRPPVASYSTVDISLRKKHYSQPLSFAVSVHNLFN
                     ADVRESSPAAVADDYPMAKRSLMAEVNYQF"
     gene            complement(1991..>2382)
                     /locus_tag="PLY05_10010"
     CDS             complement(1991..>2382)
                     /locus_tag="PLY05_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03673.1"
                     /translation="QVIQAELALGRTIYGYGRPLAKLDERIPHTLALARKLGLAEGKH
                     LQLALSVYRYLKQRKGLSMNIAAIDAALAADIGLSPEEYQLVMSPCFIAGMTPCYLDG
                     RDKPEGAFFPMRCSSVQYHGVAQREWT"
BASE COUNT          714 a          496 c          448 g          724 t
ORIGIN      
        1 gcaatgggtg ccaatgatat tagtcaagtt ttagagacag tgcctgatct tcatgtgggt
       61 atttctgacc aagtattcgc tcctaaatat aatattcgcg gtattacctc caatcgtaat
      121 gcccaaacct taatgctaat taacggtatt cccattacga atttatttac aggtaatcgc
      181 ggtcaaattt ggatgggaat gcctgtgaaa ggtgttgctc gtattgaaat cattcgtggg
      241 ccaggttcag ccttatatgg tgcagatgcg tttgcaggtg tcattaatat tattactaaa
      301 aatgctgatg atatttatgg cacaaaattg ggtgctggtg taggtaattt tggccaacgt
      361 agtgcttgga tgcaacatgg ctctactcat aactcttata aaacttcttt taatttagaa
      421 gcttataaaa cagagggttg ggactctatt attaaaagtg atgcacaaac tttaaacgac
      481 caagcttttc aaaccaatgc ttctctcgct ccgaataaag ttaatactat gaaagaggtg
      541 cttgaagcac gttttgaact aacaggttta aacgatatat taagaatagg ctatcaaggc
      601 aattataata taggaacggg agctggctta attcaagcct tagaccccga aggccgcttt
      661 aaagctgaac gttttaatgt tgattatgcc tacaacttta atgaattagt cgaaaattgg
      721 caaatacaaa cccgcaccag cttttatcgc agcacgcaac aggtagagaa aaatttactc
      781 ctttttccgc gtaatttcgc cctgtctttt gatggcaata aaaccttttt taagtatcct
      841 gatggtttta ttggtaatcc atccctcaaa gaagaccacg cccgctttga tgtcaatggc
      901 ttatttaagg gcttaaacaa gcatttaatt cgagtaggaa ctggctttta ttggggtgat
      961 atttttgaag tgacagaaag taaaaacttt ttttccaatt acactccacg cccaagccta
     1021 acggatgtaa gtgataccaa cgatgccttt ttacccgaaa aagaacgtac aaactactat
     1081 gcttttggcc aagatgaatg gcaaattgcc aatacccttc aattaacaac aggcttacgt
     1141 tatgatcatt tttcggactt tggctcaacc attaacccac gtttggcctt aatttgggca
     1201 aatacccctg atattactac caagctattg tatggtagag ccttccgtgc gccctcgatt
     1261 tcggaattat ttgttgataa taatcctgtt caaaacggta atcccaatct aaagcctgaa
     1321 attattgata cctatgaatt agctctctct catcaggttt cagataattt actgtataac
     1381 accaatattt attattataa aatcaaagac gtttgtctgc taactgattt tgtacataag
     1441 ggcgagtata acaccaatat ttattattat aaaatcaaag actacattac ccttgtgcct
     1501 atcaatgcct ttgctaacca aatgcaaaat agtggcaaac gacatggaca cggtttgagt
     1561 gccgaagtgg actattctgc tttagacaac ctacgtcttg tggctaatta tagttatcaa
     1621 ctctctaaag atgatgcgac caaaaccgca tcaggtgaca cccccaatca tcaactgtat
     1681 ttgcgcggcg aatgggaaat cacccctgca tggttactta gccctcaagt caatgttatt
     1741 ggtcagcaaa aacgtgctgc gggtgatata cgccctcctg ttgcctcata cagcactgta
     1801 gatattagcc ttcgtaaaaa gcactattca cagcccctat cttttgctgt ctcggtacat
     1861 aatttattta atgctgatgt tagagagtct agtccagcgg ctgttgccga cgattatcct
     1921 atggccaaac gtagcttaat ggcagaggtc aattatcagt tctgaattag taggggctta
     1981 ggatattgtg ctaagtccac tctctttgtg caacgccatg atactgcacg ctgctacaac
     2041 gcattgggaa aaaagctcct tcgggtttgt ctcggccatc taaataacaa ggggtcatcc
     2101 ctgcaataaa acaaggactc ataactaatt gatattcttc gggacttaaa ccaatatcag
     2161 cagctaatgc agcatctatc gcggcaatat tcatcgacaa gccttttcgc tgttttaaat
     2221 aacgatacac cgacaaagct aattgcaaat gtttgccttc tgccaaacca agttttcgtg
     2281 ctaatgctaa agtatgagga atacgttcat ctaatttggc caaaggccga ccataaccat
     2341 aaattgttcg ccctaaagct aattcagcct gtatcacttg tt
//
LOCUS       DAOQMB010000366         2370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_28314_length_2370_cov_16.313175, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000366 DAOQMB010000000
VERSION     DAOQMB010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28314_length_2370_cov_16.313175"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1963)
                     /locus_tag="PLY05_10015"
     CDS             complement(104..1963)
                     /locus_tag="PLY05_10015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC03674.1"
                     /translation="MTNAHDAMPLSNSVLNNSEIIIENEFAALGLHPAIVKALDDLNY
                     KKPTPVQAQAIPAFLAGRDLLVSSQTGSGKTAAFMLPALQLISQKPRPEVVKPQRGVK
                     GRRPRPAPACPHLIVLTPTRELAQQVTSATTQFDKYLRRTICASIVGGMPYPRQLEML
                     AKMPDILVATPGRLLDHMNSGRIDLSQLSMLVFDEADRMLDMGFSEDIDAIVGQTPET
                     RQTLMFSATLDGRTGELAADMLRDPVRVAIAQEQMDYKRIEQRVHYVDDVAHKEKLLD
                     HLLESEDLQQMIIFTATKAEADILADDLSEKGHAASALHGDMKQSMRNRTLQGLRRGE
                     IKVLVATDVAARGIDVPTISHVVNYGLPKHGEDYIHRIGRTGRAGRSGVAINLIQHGD
                     RFKWQRIERLLPVRVEVSEVEGLEPQRAPKPKTGRDGGAGRNKDRWSNDRRERRANNG
                     GGGRRDSRIDDRAFREERAPRRDDRPRFDDSPRFEQQRPRTERFEREPVRAERFDREP
                     ARAERFERDTPRQSQGQDRFNDRDIYRDNAPRRPQAQAAQPRFNDAPYSYETERPRRS
                     PRRDDFNQEAAPRRFNEDRPAREENRSRKPAFAGTDSFKEQRPRRGSQEWVRR"
BASE COUNT          661 a          489 c          577 g          643 t
ORIGIN      
        1 ataactggat tttagacgaa ggaaaatggg cttatttaaa aattaaggta ggaaaagagg
       61 ggaaatccct gactcgttaa gagtcaggga tagggtttag cgattagcga cgaacccact
      121 cttgtgagcc acgacgtgga cgctgttctt tgaagctatc tgtaccagca aaagcaggtt
      181 tgcggctacg attttcttca cgtgctggac gatcttcgtt aaaacgacgt ggtgcagctt
      241 cttgattgaa gtcgtcacgg cgtgggctac gacgtggacg ctctgtttca tagctatatg
      301 gtgcatcgtt aaagcgtggt tgtgcagcct gtgcctgtgg acgacgtggt gcattatcac
      361 gataaatgtc acgatcatta aaacgatctt gaccttggct ttgacgtggt gtgtcacgtt
      421 caaaacgttc ggcacgagct ggctcgcggt caaaacgctc agcacgaaca ggttcacgct
      481 caaagcgctc tgtacgtgga cgttgttgtt caaagcgagg gctatcgtca aaacgaggac
      541 ggtcatcacg acgtggtgca cgttcttcac ggaaagcacg atcatcaatg cggctatcac
      601 gacgaccacc accgccattg ttggcacggc gttctctgcg gtcattgctc caacggtctt
      661 tattgcgacc tgcgccacca tcacgacctg tttttggctt aggtgcgcgt tgtggctcta
      721 aaccttcaac ttcagaaact tcaacacgta ctggcaacaa acgttcaata cgttgccatt
      781 taaaacgatc accgtgttga attaagttga tggcaacacc actacgacct gcacgacctg
      841 tacggccaat acggtggatg tagtcttcac catgcttagg taaaccgtag ttaacaacgt
      901 gagaaatagt tggcacgtca ataccacggg cagcaacgtc agtcgctact aatactttga
      961 tttcgccacg acgtaagcct tgtaaggtgc ggttacgcat agattgcttc atgtcaccat
     1021 gtaaagcgct ggcagcatgg cctttttcgc ttaagtcatc agctaagatg tcagcttcag
     1081 ctttggtggc ggtgaagata atcatttgtt gtaaatcttc actttctaac aaatgatcga
     1141 gtaatttttc tttgtgagca acatcgtcaa catagtgaac acgttgttca atacgcttgt
     1201 aatccatttg ctcttgagca attgctacac gcactggatc acgtaacata tcagcggcta
     1261 attcgccagt acgaccatct aaggttgcgg agaacattaa ggtttggcga gtttcggggg
     1321 tttgacccac aatcgcatca atatcttccg agaagcccat atctaacata cggtcagctt
     1381 catcaaaaac taacattgat aattgactta aatcaatacg gccagagttc atgtggtcta
     1441 ataaacgacc tggtgtggcg actaaaatgt caggcatttt ggctaacatt tcgagctgac
     1501 gtgggtaagg cataccacca acaatactag cgcaaatggt gcggcgtaag tatttgtcaa
     1561 attgtgtggt tgcggatgtg acttgttgag ccaattcgcg tgttggggtt aatacaatta
     1621 aatgtggaca agcaggtgca ggacgcggac gacgaccttt tacgccacgt tgtggtttaa
     1681 ccacttctgg acgtggtttt tgagaaatta attgtaaagc aggcaacata aaagcggcag
     1741 ttttgcctga gcctgtttgg ctagatacta ataaatcacg acctgctaag aaggcaggaa
     1801 tcgcttgtgc ttgtacaggt gttggttttt tataatttaa atcatcaagt gctttgacaa
     1861 tagcagggtg taagcctaaa gcggcaaact cattttcgat aataatttca gaattgttga
     1921 ggacagaatt agacaaaggc atagcatcat gagcattggt catgttagta cttctataag
     1981 ttaggactta cgcattggat gggaaaacat ctataaatca acagaataat gaatgttgat
     2041 tgtaaattgt aagtcaatag ataaccgata ttttggcgaa aggctaagtt taggagcgtg
     2101 ttgttttgag acgtgggtta taccaaaata agtgatataa aagtacacgg agggtatcac
     2161 cctataacaa gacggtcatc ctaagaaatt ttggtttacg tatgtaagcc atcacaaatt
     2221 agccgctacg gcgtcgcgcc aatcaagtca gtaccaataa ggtattgacg atagtggggg
     2281 cagattgccc aacagactta taagaggaga aatatcctcg ttaagataga gattggaggc
     2341 gatgctcaac tgcgttgtta acgcggcgcg
//
LOCUS       DAOQMB010000367         2348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_28817_length_2348_cov_14.386829, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000367 DAOQMB010000000
VERSION     DAOQMB010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28817_length_2348_cov_14.386829"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="PLY05_10020"
     CDS             <1..363
                     /locus_tag="PLY05_10020"
                     /inference="COORDINATES: protein motif:HMM:NF026954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HNC03675.1"
                     /translation="YVIGMLSGVMGAAGFSLTAPVCELLQQPELLRQELILDIEHGLV
                     GKTAIHPTQIAIIHQALQVSVEDYQAASLILNQSAPAVFQHNGAMCEPATHYRWAKRI
                     LERAYWYGMSGASEKLIV"
     gene            complement(386..>1189)
                     /locus_tag="PLY05_10025"
     CDS             complement(386..>1189)
                     /locus_tag="PLY05_10025"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC03676.1"
                     /translation="LFRLNRIGSDKKDKLTREELHTILQDVFLPAKHRNMLLGILELD
                     NITVNDIMIPRQEVIGINLDDDIEDIVDILRGTHHTRLPVYKGELNNVVGILHVRNAT
                     RFLGQDNLSKSEMMQYIREPYYVPESTPLQAQLLQFQKQKRRLGLVVDEYGVVQGIVT
                     LEDILEEIVGEFTTNIAENHQDILPHSDGYFMMDGSLSIRDINRSMHWQLPTDGAKTL
                     SGLILEQLEIIPDGVVGLRLEDFYIEVMQIRDNTIKAAKIKRLILEEVA"
     assembly_gap    1190..1289
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1518..2330)
                     /gene="ccsA"
                     /locus_tag="PLY05_10030"
     CDS             complement(1518..2330)
                     /gene="ccsA"
                     /locus_tag="PLY05_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNC03677.1"
                     /translation="MVAQFAGFLAVACYLVASGILCYRLVNKQTASKVFVLSLGAIAL
                     PLHGFNIQQHIYSAQGLDLGFFNIISLIAWLIACIHIGISSYRPLFVMSIFAFPMAAF
                     GLVLSEIFHAPYQPLTHLPKGAESHIILSILAYSVLLMAALHAVLLAIQNKSLKKHRQ
                     QHKPLLIQLMPPLQTMESVLFDFIAFGFGLLSLAIISGFISLENMFAQHVAHKTILTI
                     FAWLIFGTLLAGHHWQGWRGQRAVRFTLLGFAFLFVGFLGSKVVLELILQKV"
BASE COUNT          752 a          435 c          417 g          644 t
ORIGIN      
        1 tatgtgattg gtatgttgtc gggggtgatg ggcgcggcag gttttagttt aaccgcacct
       61 gtttgtgaat tactccaaca gcctgagttg ttacgtcaag aattaatttt agatatagaa
      121 catggtttag taggtaaaac cgccattcat cccacccaaa tagcgattat tcatcaagct
      181 ttacaagtga gtgttgagga ttatcaagcg gctagtttaa ttcttaatca aagcgcgcct
      241 gcggtgtttc aacataatgg cgcaatgtgt gaacctgcca cccattatcg ttgggcaaag
      301 cgcattttag agcgtgctta ttggtatggc atgagtgggg caagtgaaaa gctgattgtg
      361 tgattattta ggagctaatt ggctcctaag cgacttcttc taaaattaag cgtttaatct
      421 tagcggcttt aatcgtatta tcacgaatct gcatcacttc aatataaaag tcttctaaac
      481 gtaaaccaac gacaccatca ggaataattt ctaattgctc cagaattaag ccgcttaaag
      541 tttttgcacc atcagtaggc aattgccagt gcatggagcg attgatatct cgaatgctta
      601 aactaccatc catcataaaa taaccgtcac tatgcggtaa aatatcttga tggttttcgg
      661 caatgttagt ggtaaattca cccacaattt cttctaaaat atcttctaaa gtgacaatgc
      721 cttgcaccac accatattca tcaaccacta agcccaaacg gcgtttttgt ttttggaatt
      781 gtaaaagttg agcctgtagc ggagtgcttt ctggtacata ataaggctca cgaatatatt
      841 gcatcatctc gcttttgctt aaattgtctt gtcccaaaaa gcgtgtggca ttgcgtacgt
      901 gtaaaatacc aacgacatta tttaattcgc ctttatatac gggcaagcgt gtgtgatgtg
      961 taccacgtaa aatatcaaca atatcttcaa tgtcatcgtc taggttaatg ccaatcactt
     1021 cttggcgtgg aatcataata tcgttaacgg taatgttatc gagctctaaa atacctaaaa
     1081 gcatattgcg gtgtttggca ggtaaaaaca catcttgcaa aatcgtgtgt aattcttcgc
     1141 gggttagttt gtcttttttg tcactgccaa ttcgatttaa acgaaacaan nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng catttttatc acctaatttg gctttatgct
     1321 ttaagcgata gcggttcgct gtcatcactc ctgtttcgga ggcagaaaaa aaagcagaac
     1381 aagcaataaa caaaactaac aggccaaata acagccctaa attggagtcg tccaagtcaa
     1441 taaacctata ataatagcta ttacctatct aaacgatatt aggtaaaata agtcaaatat
     1501 aatcttgctt aatatgttta cactttttgc aaaatcaact ctaaaacgac tttgctgcct
     1561 aaaaagccaa caaacaaaaa ggcaaaacct aataaagtaa aacgtactgc acgttgacca
     1621 cgccaacctt gccaatgatg tcctgctaat aatgtgccaa aaatgagcca tgcaaatatt
     1681 gttaaaatgg ttttatgagc cacatgttgg gcaaacatat tttctaaact gataaaacca
     1741 ctaataatgg ctaatgacaa taaaccaaac ccaaaggcaa taaaatcaaa taaaacggac
     1801 tccatggttt gtaaaggcgg cattaattga attaacaaag gcttgtgttg ttgacgatgt
     1861 tttttgagac ttttgttttg tatggccaat aaaacagcat gaagggcagc cattaataac
     1921 acgctatagg cgagaatgga taaaataata tggctttctg caccttttgg gagatgcgtt
     1981 aaaggctgat aaggtgcatg aaatatttct gataacacta agccaaacgc cgccattgga
     2041 aaggcaaaaa tactcatgac aaataatggc ctataactac taatgccaat atgaatacac
     2101 gcaatcagcc atgcaataag cgaaataata ttaaaaaatc ctaaatctaa gccctgtgcg
     2161 ctataaatat gttgttgaat attaaaacca tgtaggggca gagcaatagc acctaaacta
     2221 agcacaaaaa ctttagaagc cgtttgttta ttgacaaggc gataacataa gatgccactt
     2281 gccaccaaat agcaggcaac cgctaaaaat cctgcaaatt gagcaaccat aatgccaaag
     2341 accaatta
//
LOCUS       DAOQMB010000368         2347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_28843_length_2347_cov_15.731675, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000368 DAOQMB010000000
VERSION     DAOQMB010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28843_length_2347_cov_15.731675"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="PLY05_10035"
     CDS             complement(<1..1583)
                     /locus_tag="PLY05_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03678.1"
                     /translation="MTWRLFWRELRAGEFTLLIAALILAVTATTTLSFFTHTLAQAIN
                     QQAARLLAADVVVVSSRPLRADWSQLAQQHNLQTSQTIEFATVAQQGDNFQLSSIKAV
                     ADNYPLRGTLSTQPKMTTRTPAQGTVWVEERLLNLLNSQVGQRITLGEAEFIIAATIA
                     VDADMGGSFSAFSPKIIINIADVPKTNAIQQGSRVSYRFLVAGKSQAVSQFSQQAKDQ
                     LNDYERLRDIHSANKQLSKPIINANSYLSLASIASVLLAGIAVALAAQRFSQRHFDSF
                     ALMRCLGYSRQQLINLYASQLAAVSGFGLVIGSSLGLAFSLLLFELLGQFLPVSDLSF
                     DFIQPLITGLSTALLTLIGFALPAFLRLAQVSPLRVMRRELTPVSLSMLSSTGIALVA
                     LFILLSLETGNITLTAIVLVGGLLLAGLLGVCCGWLLGYLRTRIKQQAFANLTRQPQQ
                     TIGQILALALGFTAVLLVMVLRSDLFQQWQTDLPPNTPNHFAVTIPAEQKDSLQQQLE
                     QRHWSHTEFYPVVRGRLIA"
     gene            complement(1580..2251)
                     /locus_tag="PLY05_10040"
     CDS             complement(1580..2251)
                     /locus_tag="PLY05_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005262377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNC03679.1"
                     /translation="MTTLTPALLVANLNKTIATTEHSLTILQQIDLRIEQGSTVAIVG
                     SSGSGKSTLLGLLAGLDTPSSGSIQLLGQELSTLSEDKRASLRQQYVGFVFQSFHLLP
                     HLTALENVLLPLSLSKNTDSAWAKQCLEQVGLGHRLHHTPKQLSGGEQQRVAIARAFA
                     CKPKILFADEPTGNLDSQTGQHIIDLLFNLNTQHGTTLVLVTHDLKLAERCQQRLTLA
                     QGHML"
BASE COUNT          711 a          497 c          521 g          618 t
ORIGIN      
        1 atggcaatca aacgcccacg aaccacagga taaaattcgg tatgcgacca atgacgttgc
       61 tcaagttgtt gctgtaggct gtctttttgt tcggcaggaa tagtgacagc aaaatgattg
      121 ggggtatttg gtggcaagtc agtttgccat tgttgaaata aatcgctgcg taataccatc
      181 actaacaaca ctgccgtaaa acccaacgct aaggccaaaa tttgaccaat cgtttgttgg
      241 ggttgacgcg ttaaattagc aaaggcttgc tgcttaatgc gagtacgtaa atatcctaat
      301 aaccacccac aacatacacc taataatcca gcaagcagca aaccgccgac taaaacaata
      361 gcggtgaggg tgatattgcc tgtttctaaa cttaataaaa taaataaggc gactaaagca
      421 atgcctgtgc tgctcagcat cgacaaagaa actggcgtta attcgcgtcg catcacgcgt
      481 aatggcgata cttgcgctag ccgtaaaaaa gcaggtagcg caaaaccaat cagcgtcagg
      541 agggcagtgc ttaaacctgt gattaagggt tgaataaaat caaaactcaa atcgctaaca
      601 ggcaaaaact gacctaataa ttcaaacaat agcagcgaaa atgccaaacc taaactacta
      661 ccaatcacta aaccaaaacc actcactgcc gctaattgac tggcatacaa gttaatcaat
      721 tgttggcgcg aatagcccaa acacctcatc agtgcaaaac tatcaaaatg ccgttggcta
      781 aaacgttgtg ctgctaaagc aacggcaatc cctgctaata acacactggc aatactggct
      841 aaacttaaat aactattggc attaataatc ggtttactga gttgtttatt ggcactgtga
      901 atatcgcgta ggcgttcgta atcattgagt tggtctttgg cttgttgaga aaattggcta
      961 acagcttggc ttttacctgc cactaaaaag cgataactca cgcggctacc ttgttgaatg
     1021 gcattggttt taggtacatc cgcaatattg ataataattt tgggtgaaaa agccgaaaag
     1081 ctaccgccca tatcggcatc aacggcaatg gttgcggcaa taataaactc cgcttcacct
     1141 aaggtaatac gttgaccgac ttggctattg agtaaattta acaaacgctc ttcgacccat
     1201 actgtgcctt gtgcgggcgt gcgagtggtc attttaggtt gtgtgctaag tgtgccacgc
     1261 aaaggataat tatcggcaac cgctttaatg ctgcttagtt gaaaattgtc accttgttga
     1321 gcaacagtgg caaattcaat ggtttgactg gtttgtaagt tatgttgttg tgccagttgt
     1381 gaccagtcag cgcgtagtgg acgactactc accactacca cgtccgcagc caataaacgt
     1441 gccgcttgtt gattaatcgc ttgggctaag gtgtgggtaa aaaaactcag agtcgtggtg
     1501 gcggtgactg ccaaaatgag tgcggcaatc agtaaagtaa attcacctgc gcgtaattca
     1561 cgccaaaata aacgccatgt cataacatgt gtccttgcgc taaggttaaa cgctgctgac
     1621 aacgctcggc aagttttaag tcatgggtca ctaatactag ggttgtgcca tgttgcgtat
     1681 tcaaattaaa caataaatca ataatatgct gccccgtttg gctatctaaa ttacctgttg
     1741 gctcatcagc aaataaaatt ttgggtttac aggcaaaagc acgcgcaatg gcaacacgtt
     1801 gttgctcacc accagacagt tgtttgggtg tatggtgcaa acgatgaccc aaaccgactt
     1861 gttctaggca ttgttttgcc catgcgctat ctgtattttt acttaatgac aagggcagta
     1921 acacattttc taaggcggtt aaatgcggta acaaatgaaa cgattgaaaa acaaaaccca
     1981 catattgctg gcgtaaacta gcacgcttat cttcggacag ggtgcttaat tcctgaccta
     2041 ataattgaat agagccagag ctgggtgtgt ctaagcctgc cagtaatccc agtaaagtcg
     2101 atttaccaga gccagaacta ccgacaatag caacggtaga gccttgttcg atacgcaaat
     2161 caatctgttg caaaatcgtc aacgaatgct ccgtagtggc aatagttttg ttaaggttgg
     2221 caacaagtaa agcgggtgtg agtgtagtca tggcaaaatt ttggcttggt atcatgttaa
     2281 tcttagtaag cgggcttaat acagcttatg cagccagtat tgtggtcttt ggtgatagta
     2341 tcagcgc
//
LOCUS       DAOQMB010000369         2340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29027_length_2340_cov_6.063020, whole genome shotgun sequence.
ACCESSION   DAOQMB010000369 DAOQMB010000000
VERSION     DAOQMB010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29027_length_2340_cov_6.063020"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="nagZ"
                     /locus_tag="PLY05_10045"
     CDS             complement(<1..961)
                     /gene="nagZ"
                     /locus_tag="PLY05_10045"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNC03680.1"
                     /translation="MNGALMLDVAGLELDAEDKEVLRHPQVGGLIFFARNFAGIEQIQ
                     ALIKSIRAIRPEIIIAVDQEGGRVQRFKDGFVKLPPMKTIGDLFSKDAHKANELAYQC
                     GWLMAAEVLAIGVDISFAPVLDVDFGLSRVIGDRSIHSQPEVATALLRSYIQGMHAAG
                     MAATGKHFPGHGSVEADSHVAIPVDNRTFAQIDAFDLVPFKALSNQLQGIMPAHVIYP
                     EVDNRPAGFSPIWLQDLLRQKMGFEGVIFSDDLSMEGAGVAGGYADRADAALEAGCDM
                     VLACNNRSGAVQILEYLEKHPRMPHQHRFNALRGKGKLAWNDLP"
     gene            1131..>2340
                     /locus_tag="PLY05_10050"
     CDS             1131..>2340
                     /locus_tag="PLY05_10050"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNC03681.1"
                     /translation="MAAITRKHILWSAAFGGLVFFSGVFAKQAAAPSPVEPNKEEVKA
                     ARSTVMMLKHLHYEHKKLNDALSSQILDRYLKDLDPSRAYFLASDIAEFEAYRYLLDD
                     DLNRGDLTHGFAIYTRYQQRFNQRLDYVLKELKKGSKNFDFTINESLEVKRETVPWAT
                     EAKELDTLWYKRLKSAVLSLKLSGKKEEEIFKLLTKRYETQRNNMNRNKADDVFQTFM
                     NAFTETYDPHTEYFSPRTSENFNINMSLSLEGIGAVLQAEDDYTKIVRLVPAGPAEKS
                     KQLKPNDRIVAVAQGDAEFVDVVGWRVDEVVDLIRGPKNSTVRLQVLPANAVDEHQTK
                     IISIVRQTIKLEEQAAQKRVMTITRQDKDYKIGVIKLPTFYADFAAMQAGDPNYRSTT
                     RDVAKLIDELK"
BASE COUNT          748 a          507 c          411 g          674 t
ORIGIN      
        1 gtggcaaatc attccatgct aattttccct taccccgtaa agcattaaaa cgatgctgat
       61 gaggcatacg tggatgcttt tctaaatatt ctaaaatttg tactgcacct gatcggttgt
      121 tacaggccaa caccatatca caaccagcct ctaaagcagc atcggctcta tcggcataac
      181 caccagcaac accagcccct tccatactta aatcgtcaga aaaaataaca ccttcaaaac
      241 ccattttttg ccgtaataaa tcttgcagcc aaattggcga aaatcctgct ggacgattat
      301 caacttctgg ataaataaca tgagcaggca taatcccttg taattgatta ctcaatgctt
      361 taaacggcac aaggtcaaag gcatcaatct gggcaaatgt acgattatcc acaggaatcg
      421 ccacatgaga gtcagcttct acagaaccat gtccaggaaa atgtttacct gttgccgcca
      481 tacctgctgc atgcatacct tgaatatatg agcgtaataa agccgttgcc acttctggtt
      541 ggctatgtat agaacgatca ccaatcaccc gacttaaacc aaaatccaca tctaatacgg
      601 gcgcaaaact aatatcaaca ccaattgcta agacttctgc tgccattaac caaccacatt
      661 gataggctag ttcatttgct ttatgagcat ctttactaaa caaatcacca atcgttttca
      721 ttggtggtaa tttaacaaag ccatctttaa aacgctgtac tcgtccgcct tcttggtcaa
      781 cagcaatgat gatttcggga cgaattgcac gaatactttt tattaaggcc tgaatttgtt
      841 ctatacctgc aaaatttctg gcaaaaaaga ttaaaccacc aacctgcgga tgacgcaaaa
      901 cctctttgtc ctctgcatct aactccaaac ctgctacatc aagcatcaat gcgccattca
      961 ttttttagct cctatttagg gataattaaa attatgacta attttaatta aattttctct
     1021 gtatattctg tgttttttcg caatataacc gctacaaaat tcagcaatga ctctctataa
     1081 tcaatcctaa gtcagtagcc cgctgacact ttgtaaatat taagaatcac atggccgcaa
     1141 ttactcgtaa acatatttta tggtcagccg cttttggtgg cttagtcttt ttttcaggtg
     1201 tgttcgccaa acaggcagca gctccatcac ctgttgaacc caacaaggaa gaagttaagg
     1261 ccgcacgctc aaccgtgatg atgctcaagc atcttcatta tgaacacaaa aaactcaatg
     1321 atgctttatc aagccaaatc ttagatcgtt atcttaaaga tttagaccct tctcgtgctt
     1381 acttcttggc aagtgatatt gccgaatttg aagcctatcg ctacttatta gatgacgact
     1441 taaaccgtgg tgatttaaca catggttttg ctatttatac acgctatcaa caacgtttta
     1501 atcaacgttt agattatgtg ttaaaagagc taaaaaaagg tagcaaaaat tttgatttta
     1561 cgatcaatga aagcttagag gttaaacgcg aaacggtgcc ttgggcaacc gaagctaaag
     1621 aattagatac tttatggtac aaacgcttaa aatcagcggt gttaagcctt aaattatcag
     1681 gtaaaaaaga agaagaaatt tttaaattat tgactaaacg ttacgagact caacgtaaca
     1741 atatgaatcg taataaagca gatgatgtgt ttcaaacttt tatgaacgca tttactgaaa
     1801 cttatgatcc ccataccgag tatttttcgc cacgtacctc tgaaaacttc aatattaata
     1861 tgagtttatc tcttgaaggg attggtgccg tattacaagc tgaagatgac tataccaaaa
     1921 ttgttcgtct agtccctgca ggacctgctg aaaaatctaa acagcttaaa cccaacgacc
     1981 gtattgtggc tgttgcccaa ggtgatgctg agtttgttga tgtcgttggt tggcgtgttg
     2041 atgaggtggt cgatttaatt cgtggcccca aaaatagtac tgtacgttta caagttttac
     2101 ctgctaacgc agtagatgaa catcaaacca agattatcag tattgttcgt caaaccatta
     2161 agctcgaaga gcaagccgca caaaaacgtg taatgaccat aacacgccaa gacaaagact
     2221 ataaaattgg tgtaattaag ctgccaacgt tttatgccga ttttgcagcc atgcaagcag
     2281 gagaccctaa ttatcgcagt accacgcgtg atgtggccaa actgattgat gaactaaaag
//
LOCUS       DAOQMB010000370         2336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29118_length_2336_cov_16.425690, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000370 DAOQMB010000000
VERSION     DAOQMB010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29118_length_2336_cov_16.425690"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLY05_10055"
     CDS             <1..237
                     /locus_tag="PLY05_10055"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNC03682.1"
                     /translation="MIYESLTAGAQVGVFAVPRLQKDRITQAVDDLAAQEKLVLFNQW
                     QQNQQMADKQSFCEAQRAAQWLLEQEGLWPNAIK"
     gene            216..1271
                     /locus_tag="PLY05_10060"
     CDS             216..1271
                     /locus_tag="PLY05_10060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC03683.1"
                     /translation="MAKRYKIVHVMATAGGVVGGLEKHTLELCAALARKHEVHLLADR
                     AYQGVCDKRIHFHPINFQQSRWNPFLYWQIAQTINQIQPNIVHAQAGKAASIIRWLKW
                     LFKQVKFVATVHGTKKDISAYAAMDGVITVSRQLAEAFGNHPHLKVIYNGARASPKLN
                     GRERKLLRAELLAAEDLPIVMAVGRLAPVKAFDVLLKAFVGIDARLVIVGDGDERESL
                     QQLAEQLGLSHRVLFLGHRQDVGSLLQVADLCVISSHQEDYPLVMIEALQQGCLLIST
                     AVSGVKEILPPALLTAPNDVQELHNLLYVTLPRIETLRSNFLPLFLRAQQELTIEGMT
                     ERTLAFYSELLENTHEG"
     gene            1261..>2336
                     /locus_tag="PLY05_10065"
     CDS             1261..>2336
                     /locus_tag="PLY05_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNC03684.1"
                     /translation="MKAKVQTVQPVTASMWLLAFALSTPILLLPFARAFIAPLGVLAV
                     MGLFMLIGLLRHRGTFNSEDKALQVLPRVFLFIWLPMLISLIDAEYPKQVLKAVQLYP
                     LYTLMALAVIVILRANNVLKPLAIIISWIVCIWAFDGVAQAALGIDMFNIPLDRAGVD
                     SAKANAFFTSPNKYGFYMGMMAATPLFTMYLCGVNRLTHILVSLLLMASVLVGASRGG
                     WVMFACAFVIYVYLVFIKPAKRPLVPILVIPSLIAVVFMIALQFGTGMQERMSRFNGL
                     LEKVDYQTLNIALSGRVDIYQAAWEIGKQHPINGVGVDSFDKVYLSYLPQNNMWQTMI
                     VDAPPHPHQVMLEIWSGAGSIGIIG"
BASE COUNT          661 a          397 c          508 g          770 t
ORIGIN      
        1 atgatttatg aaagtttaac agcaggtgct caagttggtg tattcgctgt acccagatta
       61 caaaaagatc gtattactca agcagtggat gatttagccg cgcaagaaaa attagtgtta
      121 tttaatcagt ggcagcaaaa ccaacaaatg gcagataaac aatctttttg cgaagcacaa
      181 cgtgcggctc aatggttgtt ggagcaagaa gggctatggc caaacgctat aaaatagtac
      241 acgtcatggc cacagcaggt ggcgtggttg gtggtttgga aaaacatact ttagagcttt
      301 gcgcagcttt agcacgcaaa catgaggtac atttattggc ggacagagcc tatcaaggtg
      361 tatgtgataa acggattcat tttcacccaa ttaattttca acaaagtcgt tggaatccct
      421 ttttatattg gcaaattgca caaacgatta atcagattca acctaatatt gtccatgctc
      481 aagcaggtaa agcagccagt attattcgtt ggttaaaatg gttatttaaa caagttaaat
      541 ttgtggcaac cgtacatggc actaagaaag atattagcgc ttatgcggca atggatggtg
      601 tgattacggt gagtcggcag ttggcagagg cctttggaaa tcatcctcac cttaaagtta
      661 tttataatgg tgcaagagct agccccaaat taaatggccg agagcgtaag ttattacgcg
      721 ctgagttatt agcggccgaa gatttaccca ttgtcatggc tgttggtcgt ttagcacctg
      781 ttaaagcctt tgatgtgtta ttaaaagcct ttgtgggtat tgacgctcga ttagtgattg
      841 tcggtgatgg agatgagcga gagtctctac aacaattagc tgaacaatta ggtttaagtc
      901 atcgagttct ttttcttggc catcgacaag atgtaggaag tttattacaa gtggctgatt
      961 tgtgtgtgat ttcttcgcat caagaagact atccattagt catgattgaa gccttacaac
     1021 aaggttgttt gttaatttca acggcagtga gtggtgttaa agaaattctg ccacctgctt
     1081 tattaactgc gccaaatgat gtacaagaat tacataatct actctatgtt acactaccta
     1141 gaattgaaac cttacgttca aattttctcc ccctattttt acgtgcgcaa caagaattaa
     1201 ccattgaggg gatgactgaa cgaacactcg cattttattc tgaattattg gaaaacaccc
     1261 atgaaggcta aagttcaaac ggtacaaccc gttactgcaa gtatgtggtt gctagcattt
     1321 gctttaagta cccccattct tttattacct tttgctcgtg cttttattgc acctttaggc
     1381 gtattggcag tcatgggctt atttatgctg attggtttat tacgtcatcg gggaacgttt
     1441 aatagtgaag ataaagcatt acaggtatta ccgcgcgtat ttttgtttat ttggcttccg
     1501 atgctgattt cattaattga tgcagaatat cctaagcaag tccttaaagc agtacagtta
     1561 tatccgcttt atacgttgat ggccttggct gttattgtta ttttacgtgc taacaatgta
     1621 ttaaagcccc tagctatcat catcagttgg attgtttgta tttgggcatt tgatggtgtt
     1681 gcacaagcag ctttaggcat agatatgttt aacattccat tagatcgagc aggtgtggat
     1741 tcggcaaaag cgaatgcttt ttttacttca cctaataaat atggatttta tatgggaatg
     1801 atggcagcaa cacctttatt taccatgtat ttatgtggag taaatcggct gactcatatt
     1861 ttagtcagct tgttattaat ggcctccgta ttagttggtg catcacgcgg tggttgggta
     1921 atgtttgcct gtgcttttgt tatttatgtg tatttggtat ttattaaacc tgcaaaacgc
     1981 ccattagtac ctattttagt cattccaagt ttaattgctg ttgtgtttat gatcgctttg
     2041 caatttggca caggtatgca agaaagaatg agtcgtttta atggcttatt agaaaaggtt
     2101 gattatcaaa cattaaatat tgcattaagt ggtcgagttg atatttacca agcagcatgg
     2161 gaaattggta aacagcatcc aattaatggg gtaggtgttg atagttttga taaagtttat
     2221 ttatcctact tacctcaaaa taatatgtgg caaacgatga ttgttgatgc acctccacat
     2281 ccacatcaag tgatgttaga aatttggtct ggtgcaggtt ctatcggcat tatagg
//
LOCUS       DAOQMB010000371         2331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29235_length_2331_cov_8.095782, whole genome shotgun sequence.
ACCESSION   DAOQMB010000371 DAOQMB010000000
VERSION     DAOQMB010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29235_length_2331_cov_8.095782"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PLY05_10070"
     CDS             <1..1405
                     /locus_tag="PLY05_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC03685.1"
                     /translation="HNTLTSYLVRVLLVMTGNYGRKGGNIFVQLFGPNMPYWSTMTKA
                     LESSIESIPALLPIPQLPPAIIPEEILSQHPDRIRALICDGANPIASYPDTKRFKEAF
                     AQLDLLVVIDPAMSETAQMADYVLPSCTGYEKWEFSIFPKDIIAPQVRPPVVNPIGEA
                     LPEVEIYYRLARAMGLVGAAPKFLHRLAKQARKPLMTPAYLAAVNSYALASSKLQGVG
                     AIVAHSAFLMYETLGKTLPNPMLAYIWLLTLGYATTRRDQVIRALPEAKAMLNPFALT
                     EYLFAKILANPQGVLLGHYEIEHNFEYYSKHQDGKIRLYFADYAQDLSRLIAQDVEPD
                     EKDYPFVLNGGLRTGFTANTIMRDPSWRKGRAIHASLYMSEADAAALNAKEGDLVKIS
                     SKRSSVIAPVKVDKNTLTGHLHLPNILGQYYPDPVTGELKANGIAINELVDLTDRDPY
                     TGCPHTKRIRCKVELFN"
     gene            1425..>2331
                     /locus_tag="PLY05_10075"
     CDS             1425..>2331
                     /locus_tag="PLY05_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020443459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HNC03686.1"
                     /translation="MRYLRTPDSCFANLPEYPFVPHYLMVDDTEGGQLRMHYLDEGER
                     QAPVVLMLHGEPSWSFLYRKMIPLVVAAGYRVIVPDLIGFGRSDKPTEQSDYSHSRHI
                     FWVDSLLKQLQLKDMVLVCQDWGGLIGLRLVAEQPELFAGVVAANTMLPAFPLKHPNL
                     RAAFSKWQQLRATVGFGSWFSFSQLYPSWTAAFVINLGTALRLSPEIKAAYNAPYPDQ
                     TYMAGARVFPRLVPSEMKQNSQAWKKLINFNKPFLCAFSDKDPIMSSMAGVFPTLVAG
                     CRGQSHTTIYNGGHFLQEDQGEQLAE"
BASE COUNT          642 a          456 c          511 g          722 t
ORIGIN      
        1 acataacacc ttaacatctt atttagtgcg cgtgttatta gtgatgaccg gtaattatgg
       61 ccgaaaaggg ggtaatattt ttgtgcagtt atttggccca aatatgccat attggtcaac
      121 gatgactaaa gctttagagt cttctatcga gtcaattcct gctttattac cgattccaca
      181 attaccaccc gccattattc ccgaagagat tttatcgcag catcctgacc gtatccgtgc
      241 cttgatttgt gatggagcca atccaatcgc cagctatcct gataccaaac gttttaaaga
      301 agcctttgct cagttagatt tattggtggt gattgaccca gccatgagcg aaaccgcaca
      361 aatggccgat tatgttttgc ccagttgtac aggttatgaa aaatgggaat tttcgatttt
      421 tcctaaggat attattgcgc cacaagtacg cccacctgtg gttaatccta tcggtgaagc
      481 cttacctgaa gtagaaattt attatcgttt ggcacgagct atgggcttag tgggggctgc
      541 acccaaattt ttacatcgtt tagccaaaca agcgcgcaaa ccgttaatga cacctgctta
      601 tttagccgca gtcaatagtt acgccttagc cagtagcaaa ttgcaaggtg taggtgcgat
      661 tgttgcccat tcggcttttt taatgtacga aaccttaggt aaaaccttac ctaatccgat
      721 gttggcgtat atttggttat tgactttagg ttatgcaacc acacgtcgtg accaagtcat
      781 tcgtgctttg cctgaagcca aagcgatgct taatcctttt gctttaacag agtatttatt
      841 tgccaaaatt ttggctaatc cacagggcgt tttattaggc cattatgaaa tagagcataa
      901 ttttgaatat tactccaaac atcaagatgg caaaattcgt ctatattttg ctgattatgc
      961 tcaggattta agtcgtttga ttgctcaaga tgtagagcct gacgaaaaag attatccttt
     1021 tgtcttaaat ggtggattaa gaacaggttt tactgccaat accattatgc gtgacccgag
     1081 ttggcgtaaa ggacgtgcta ttcatgcctc gttatatatg tcggaagcag atgccgccgc
     1141 tctaaatgcc aaagaaggcg atttagtcaa aatcagctcc aaacgttctt cggtgattgc
     1201 acctgttaaa gtcgataaaa ataccttaac aggccatttg catttaccta atattttagg
     1261 gcagtattat cccgatccag tcacaggcga attaaaagcc aacggtattg ccattaatga
     1321 gttagttgat ttaaccgata gagaccctta tacaggttgt ccgcatacca aacggattcg
     1381 ttgtaaagtg gaattgttca attaaaacaa aaacgaggaa ccctatgcgt tatttaagaa
     1441 ctccagatag ctgttttgct aacttacctg agtatccgtt tgtgccacat tatctgatgg
     1501 ttgatgatac cgaaggtggc caattgcgta tgcattatct tgatgaaggt gagcgtcaag
     1561 cacctgtggt gttaatgctt catggtgagc ctagttggag ttttttatat cgtaaaatga
     1621 ttcctttggt agttgcggca ggctatcggg tgattgtgcc agatttaatt ggttttggtc
     1681 gttctgataa gcctactgaa caaagcgatt atagtcatag tcggcatatt ttttgggtgg
     1741 atagtttatt aaaacaatta caacttaaag atatggtgtt ggtgtgccaa gattggggtg
     1801 ggttgattgg cttacgctta gtggcagagc agcctgaatt atttgcaggg gtggtggcag
     1861 caaataccat gttgccagca ttcccattaa aacatcccaa tttacgagct gcttttagca
     1921 agtggcaaca acttagagca accgtgggtt ttggcagttg gtttagtttt tcccaattat
     1981 atccatcatg gacagccgcc tttgtgatta atttaggtac ggctttacgt ttatcaccag
     2041 aaattaaagc cgcttataat gcaccatatc ctgaccaaac gtatatggca ggggcgcgag
     2101 tttttccacg tttagtgccc agcgaaatga agcaaaatag ccaagcatgg aaaaagctga
     2161 tcaattttaa taaacctttt ttatgtgctt ttagtgataa agacccgatt atgtcgtcaa
     2221 tggcgggagt tttccctaca ttagtggcag gatgtcgcgg ccaatctcat actaccattt
     2281 ataatggcgg ccactttttg caagaagacc aaggtgaaca attggcggag g
//
LOCUS       DAOQMB010000372         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29530_length_2317_cov_12.438108, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000372 DAOQMB010000000
VERSION     DAOQMB010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29530_length_2317_cov_12.438108"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..830
                     /locus_tag="PLY05_10080"
     CDS             42..830
                     /locus_tag="PLY05_10080"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNC03687.1"
                     /translation="MRLSTNLTIKFSTLLVLSQLAWSGQAEDIAQEADKRVHGYGDST
                     ANLKMTLISASNETSTRQLRVKSLDLGKDGDRSLLIFDTPKDVSGTALLSHTQATGDD
                     LQWLFLPSLARVKQISGRNKSGPFMGSEFSFEDMVISYWQKYSYKLLKEEKCAANNNL
                     TCFVLEKTPLDTNSGYSKQLVWIDKQDYLQHRIEFYDRKSSLAKVYTASDFKKYQANL
                     WRPSSMLMVNQQTRRQTRLDWSDYQFKTGLNKDDFTENALKRQK"
     gene            903..2183
                     /locus_tag="PLY05_10085"
     CDS             903..2183
                     /locus_tag="PLY05_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03688.1"
                     /translation="MLLRHVCHSIFALSLLSSVAQAYELKSELRLESTNFFEQGAQQQ
                     SQFTGSIAAQVEVNQALTNSQQLQLRVFGRVDEQDNHRSGLDVREALWTYAQQAWQVR
                     AGVGQVFWGTTEAQHLVDIVNQTDYLASLDGDVKLGQPLVNVSWEQNQHLVDFYVLPY
                     FRERPFAGADGRLRLPYVIDSEASYESSAKRQHLDVAFRYQFNQQNLRVGLSGFSGTS
                     REPILSPVIDPSQFSYMATPFGFVPTGFVGNYQPYLQVYYPIIQQLGIDAQYIIGDCL
                     LKLEAIDRHGFGQRYQGVDTGLEYTQVRAFDSNVDVGWLAEYLYDSRNNTATTSFEHD
                     VFVGWRFAFNDASSSELLTGVISDIHSHEQAWKIKGSTRLNDSSKISMEGRFFHTKQD
                     VPSPFSALSTQPSDEKLYPLTDDSFLRAEFSYYF"
BASE COUNT          695 a          454 c          460 g          708 t
ORIGIN      
        1 gaatttaatc accttatatt atacagatat gcaaggcata catgcgcctg tcaaccaatt
       61 taacaattaa attcagcacc ctattagttt taagtcaatt agcgtggtct ggtcaagccg
      121 aagatatagc ccaagaagca gataagcggg ttcatggtta tggtgattct accgccaatt
      181 taaaaatgac cttaatcagt gctagtaatg aaacctctac tcgtcaatta cgtgttaaaa
      241 gcctagattt aggcaaagat ggtgatcgta gcttattaat ctttgatacg cctaaagacg
      301 tttctggcac tgccctgtta tctcataccc aagcgactgg tgacgattta caatggttat
      361 ttttaccgag cttggcacgt gttaaacaaa tttctggccg caataaatct ggcccattca
      421 tggggtcaga gttttccttt gaagatatgg tcatttctta ctggcaaaaa tacagctaca
      481 aactgcttaa agaagaaaaa tgtgctgcta ataataattt aacgtgtttt gttttagaaa
      541 aaacgccgtt agataccaac tctggctata gcaaacaatt agtgtggatc gataaacaag
      601 attatttgca gcatcgtatt gaattttatg atcgcaaaag tagcttagcc aaagtgtata
      661 ccgccagtga ttttaaaaaa taccaagcca acttatggcg accaagctcc atgttaatgg
      721 tgaatcaaca aacacgtcgg caaacccgtt tggattggtc ggattaccaa tttaaaacag
      781 gcttaaacaa agatgacttt actgaaaatg ctttaaaacg acaaaaataa tttttttgat
      841 gaaatttaat caataagtcg ttcgttgtgt ttaaaatcct aacattttaa tatgagtttt
      901 aaatgttact acgtcatgtt tgccacagta tttttgcgtt aagcctcctc agcagtgttg
      961 ctcaagctta tgagcttaaa tcagaattac gtcttgaaag cactaatttt tttgagcaag
     1021 gcgcacaaca gcaaagccaa ttcacaggct caattgcagc tcaagttgaa gtcaatcagg
     1081 ctttaaccaa tagccagcaa ttacaactac gcgtttttgg ccgtgttgat gaacaagata
     1141 atcatcgctc tggattggat gtgcgtgagg ctttgtggac ctatgctcag caagcgtggc
     1201 aagtgcgtgc aggtgttggc caagtatttt ggggaacaac cgaagcacaa catttggtgg
     1261 atattgttaa tcaaaccgat tatttggcta gcttagatgg cgacgttaaa ttaggtcagc
     1321 ctttagttaa tgtcagttgg gaacaaaatc agcatttagt ggatttttat gttttaccct
     1381 attttagaga gcgtccattt gcaggggcag atggtcgttt acgtttgccc tatgtgattg
     1441 actctgaagc aagttatgaa tcaagcgcaa aacgtcagca ccttgatgtt gcctttcgtt
     1501 atcaatttaa ccaacaaaat ttacgggtag gtcttagtgg ttttagtggt acatcgcgcg
     1561 agccaatctt aagccctgtt attgacccaa gccaatttag ctatatggcc accccttttg
     1621 gttttgtacc aactggcttt gttggcaatt atcagcccta tttacaagtt tattatccaa
     1681 tcattcaaca acttggcatt gatgctcaat acattattgg cgactgttta ttaaaactcg
     1741 aagcgattga tcgacatggt tttggtcaac gttatcaagg cgtagacaca ggcttggaat
     1801 atacccaagt tcgcgctttt gacagcaacg ttgatgttgg ttggttagct gaatatttat
     1861 atgacagtcg caataacacc gccaccacca gttttgaaca tgatgtgttt gtgggttggc
     1921 gttttgcctt taacgatgcc agctctagcg aattattaac aggtgtgatc agcgacattc
     1981 actctcacga acaagcatgg aaaattaaag gcagcactcg cctcaatgat tccagcaaga
     2041 tcagcatgga aggtcgtttt tttcacacca aacaagacgt acctagccct ttttctgcat
     2101 taagcacgca gccttcagat gaaaaactgt atcccttaac cgatgacagt tttttacgtg
     2161 ccgaatttag ctattatttt tagagattaa tcatgaaaaa accccataac ctgactttac
     2221 aagatgcgtg gccaaaacgc tttgttggct ggatgaatga acgttttccg ttccataaaa
     2281 cttatcaaaa cctacttatt gtagaacaaa taggctt
//
LOCUS       DAOQMB010000373         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29587_length_2315_cov_5.393805, whole genome shotgun sequence.
ACCESSION   DAOQMB010000373 DAOQMB010000000
VERSION     DAOQMB010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29587_length_2315_cov_5.393805"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="PLY05_10090"
     CDS             <1..967
                     /locus_tag="PLY05_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03689.1"
                     /translation="GLSAVLQARVHAIREATDQLNEGVHLNNMDMMRHAAINLAELFR
                     QISQQLDQDRHAILEIAEKAKATDSQLSATYRYRQVLQAYDKYVEPMAQMMDTGIHGT
                     FYPYLEQAERVLDNAVERLTTQGSLYSQRQHIRQVAFATKDLRQLGREVLKQCSDTLL
                     PLREEIRQHNQLSTSISYLLSQVRKRSLNKTFRFASLPLWQRDMPRRVTVGDEVLTIM
                     AEAKNYQPISIGFPEEMTEISQTVLDIVDDIAVSQHLFSQLPVKDVLSWLQQHYGYLS
                     DSTLLRLYHYLIQQEAWLCFQAEQAVLCELKQINVYHYPHGVASH"
     gene            964..1539
                     /locus_tag="PLY05_10095"
     CDS             964..1539
                     /locus_tag="PLY05_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03690.1"
                     /translation="MNLLTINLAKLHKLSELFQFFNSGKHLNRLSEPELWAALEQEQN
                     QYELLFEHLGYRLRVDGRGFAWFHTEDSNSNVSKATRNLALVLMVLFDYQADNQQSLA
                     RFCDWRIDRVLLQAVFDKHRELLLAESLDIESITQVYDTAVRYGFAQAQDSTWRLLPA
                     AVRYLDHFQELARHQMTENELMDAAEEEQVS"
     gene            1536..>2315
                     /locus_tag="PLY05_10100"
     CDS             1536..>2315
                     /locus_tag="PLY05_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03691.1"
                     /translation="MITYGFQRLVLLNSAGYQRAELPLDASVSLVAPNNTGKTSLINA
                     LQFLLIVDKRRMDFGANDLDKSRRFYFPNNSAYILLEVFLPDSGTVVLGCVGKGVSHD
                     YEYFAYQGQLNIEEYRLADGSLVSQPQLKNQLLQYQRLVFSYNTSDFIEVLYGRRKKR
                     VANEPDFAVFKLEHSSQVDAFQRVLTRTLRLDKLRSHEVKAYLLSIFKRDLPDANIDF
                     KKEWDTAFAEVNREREKYQAAVKQQAEIAQLEQKQQSRLQIR"
BASE COUNT          739 a          391 c          470 g          715 t
ORIGIN      
        1 aggtttatcg gctgttttac aagcgcgagt acacgctatt cgagaagcaa cagatcaact
       61 taatgagggc gtgcatctca ataatatgga tatgatgcgt catgcagcga ttaatttagc
      121 ggaattattt cgacaaatca gccaacagct tgaccaagac cgtcatgcta ttttagaaat
      181 tgcagaaaaa gcaaaagcaa cagatagtca attatctgcc acatatcgtt atcggcaagt
      241 gctacaagcc tatgataaat atgtagagcc tatggcacaa atgatggata caggcattca
      301 tggtacgttt tacccttacc ttgagcaagc tgaacgcgtc ttagataatg ctgtagaaag
      361 attgaccacc caaggctcgt tatatagcca acgccaacat attcgccaag tagcttttgc
      421 taccaaagat ttacgacagt tgggacgtga agtcttaaaa caatgtagtg atacattact
      481 ccccttacgt gaggaaataa gacaacataa tcaattaagt acgtcaatta gctatttatt
      541 aagccaagtt cgtaaacgta gccttaataa aacgtttcgt tttgcatcac taccattatg
      601 gcaacgtgat atgccaaggc gagtcaccgt aggcgacgaa gtattaacga tcatggcgga
      661 ggcaaaaaat tatcagccaa tcagtattgg ctttcccgaa gagatgactg agattagcca
      721 aacagtatta gatatagttg acgatattgc ggtgagtcag catttatttt ctcaattacc
      781 tgtgaaagat gttttatctt ggttgcaaca gcattatggt tatttaagcg attcgacttt
      841 gttgcgttta tatcattatt taatacagca ggaagcatgg ctgtgttttc aagctgagca
      901 agctgtattg tgcgagctta aacaaattaa tgtctatcat tatccacatg gagtggcatc
      961 acattgaatt tactcacgat taatttagct aaacttcata agttaagcga actatttcaa
     1021 ttttttaaca gtggtaaaca cctaaaccgt ttaagtgagc ctgaattatg ggcagcttta
     1081 gaacaagaac aaaatcagta tgaattatta tttgaacatt taggttatcg cttgcgtgtt
     1141 gatggacgtg gttttgcatg gtttcatacc gaagatagta atagtaatgt gtctaaagca
     1201 acacgcaatt tggcattagt gttaatggta ttatttgatt atcaagctga taaccaacaa
     1261 tctttagcac gtttttgtga ttggcgaatt gatagagttt tgctacaggc agtttttgat
     1321 aaacaccgtg agttgttact ggctgaatct ttagatatag aaagtatcac tcaagtttat
     1381 gataccgccg tacgttatgg ttttgctcaa gcacaagata gcacatggcg tttattacct
     1441 gcggcagtac gttatttaga tcatttccaa gagttagcac gacatcaaat gacagaaaat
     1501 gagctaatgg atgcagcaga ggaagagcaa gtatcatgat aacctatggt tttcaacgtt
     1561 tagtattgtt aaacagtgca ggatatcaac gcgcagagtt acccttagat gcctctgttt
     1621 ctttagtggc acccaataat acaggtaaaa ctagtctgat taatgcttta caatttttgt
     1681 taattgttga taaacgtcgt atggattttg gtgcaaatga tcttgataaa agccgtcgat
     1741 tttattttcc aaataacagc gcatatattt tattagaagt ttttttgcca gattcaggta
     1801 cagtggtatt aggttgtgtt ggcaaaggtg taagccacga ttatgaatat tttgcttacc
     1861 aaggacagtt aaatattgaa gaatatcgtt tggctgatgg cagcttagtg tcacaaccac
     1921 aacttaaaaa tcagttatta caatatcaac gtttggtgtt ttcttataat acaagtgact
     1981 ttatagaggt attatatggt aggcgtaaaa agcgtgttgc aaacgagcct gattttgcgg
     2041 tgtttaaatt agaacatagt agccaagtag atgcatttca acgggtatta acacgtactt
     2101 tacgccttga taagttgcgt tcccatgaag ttaaagccta tttattaagt atttttaaac
     2161 gtgatttacc cgatgcaaac attgatttta aaaaagaatg ggatactgct tttgctgaag
     2221 ttaatcgtga gcgtgaaaaa tatcaagctg ccgttaagca acaagccgag attgcacaac
     2281 tagagcaaaa acaacaaagt cgtttacaaa tacgt
//
LOCUS       DAOQMB010000374         2305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29817_length_2305_cov_9.681333, whole genome shotgun sequence.
ACCESSION   DAOQMB010000374 DAOQMB010000000
VERSION     DAOQMB010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29817_length_2305_cov_9.681333"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..1056
                     /locus_tag="PLY05_10105"
                     /pseudo
     CDS             210..1056
                     /locus_tag="PLY05_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249535.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
     gene            1058..2164
                     /locus_tag="PLY05_10110"
     CDS             1058..2164
                     /locus_tag="PLY05_10110"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC03692.1"
                     /translation="MAIPFLDLKTINAQMQEEIEQAALRVLRSGWYILGEELQNFEKQ
                     FAQWCGVEHVIGVANGLDALTLTLRAWKELGLIKEGDEVIVPANTYIASILAITENRL
                     KPVLVEPDTITFNIDINKIEAVITSKTRVILPVHLYGRLVDMPALLKLAKQYDLLVLE
                     DAAQAHGASLEGKKAGAWGDSAGFSFYPGKNLGALGDAGAITTNNLELANVLRALRNY
                     GSHKKYHNQYQGVNSRLDEIQAAILAVKLKYLDMQTRQRQAIASAYQQYIANPDIVLP
                     HAGVEQQHVWHLFVIKSDKRQQLQAYLHSHGIETGIHYPIAPHQQPAYACFKHMSLPI
                     SEKLHQQVLSLPIWPNMTQQQIERVVSVCQEFSA"
BASE COUNT          728 a          331 c          498 g          748 t
ORIGIN      
        1 aaaagacagt ttttattggt ttttagtgtc ataatccgca cttttgctaa gtatgtagct
       61 tataaaattg ggcgttatga acgattatta ccattaggct taaaacgttt tttaagtatg
      121 catcgtggtt attggaagca gtgagttaaa ttactcatta aattttgagg ttgtcaatga
      181 ttaaagagct aaaggctgga tggcataaaa tggattttat ggttatgggt gatgagcgtg
      241 gaagcctaac agcattagag tccactaaaa atataccttt tcaaatacag cgagtttatt
      301 acatctatca taccttacct aatgtaagaa gaggcttgca tgctcataaa gatttgcaac
      361 aagtagcgat ttgtgtagct ggaagttgta aatttttggt agatgatggt aaaaacaaag
      421 aagtcatctt gttggataaa gcgactaaag gcctttatat tggtgcgatg gtatggcgag
      481 aaatgtttga ttttacagaa gattgtgtct taatggtatt agcaaatcga cattatgaag
      541 agtctgatta cattcgctct taccaacatt ttttggagtt agtaccaaat ggttgagata
      601 catcctttag ctgatgttaa gcaagccact attggtaata aaacccgtat ttggcagttt
      661 gccattgtgt tagatggtgc tgtaattggt gaagattgta atatttgtgc gcatactcta
      721 atagaaggaa aagtggttat tggaagttat gtaactatta aaagtggtgt gtacatttgg
      781 gatggtatta ccattgagga ttatgttttt ttggggccaa atgttacttt tactaatgat
      841 ttgtttccta ggtctaagca atatccttta gagtttgtta agacaaaaat ttgtcgaaat
      901 gcttctattg gtgctaatgc gactattttg gcgggtataa caataggtga atatgcaatg
      961 gttggagctg gctctgtagt aaccaaagat gttcctccat atgcggttgt tgttggcaat
     1021 ccagctcgta taatgcgttt tttagagaaa aattaaaatg gccattcctt ttttagactt
     1081 aaaaacaatt aatgcgcaaa tgcaagaaga aattgagcag gcagctttaa gagttttgcg
     1141 ttcgggttgg tatattttag gtgaagagtt acaaaatttt gaaaagcaat ttgctcaatg
     1201 gtgtggtgtt gagcatgtta ttggtgtggc aaatggttta gacgctttaa cgctgacttt
     1261 aagagcatgg aaagagctag gattaataaa agaaggtgat gaagttattg ttcctgctaa
     1321 tacgtatatt gcgagcatct tagcaattac ggaaaataga ttaaaacctg tactcgttga
     1381 accagataca atcaccttta atattgatat aaacaaaata gaagcggtta ttacttcaaa
     1441 aacgcgagtt attttacctg tacatttata tggtcgttta gttgatatgc cagcattatt
     1501 aaaactggcg aagcagtacg atctgttggt gttggaggat gctgcacaag ctcatggagc
     1561 ttcacttgaa ggtaaaaaag caggtgcgtg gggggattcg gctggcttta gtttttatcc
     1621 tggtaaaaac ttaggagcat tgggagatgc aggagcaatt acaacaaata acttagaatt
     1681 agcgaatgtt ttgcgcgcat tacgtaatta cggctcacat aaaaaatatc ataatcaata
     1741 tcaaggggtg aacagtcgct tagatgaaat tcaagcggca atcttagcgg taaaattaaa
     1801 atacttagat atgcaaaccc gacaacgtca agcaattgca agcgcatatc aacagtatat
     1861 tgctaatcct gatattgtat tgcctcatgc tggtgtagaa caacaacatg tttggcattt
     1921 atttgttatt aaaagtgata aacgtcaaca attacaagca tatctacatt cacacggtat
     1981 tgagacaggt attcattatc ctattgcacc tcaccaacag cctgcttatg cttgttttaa
     2041 gcatatgtct ttgccaataa gtgaaaagtt acatcaacaa gtcttgagtt taccaatttg
     2101 gccaaatatg acacaacaac aaatagaacg tgttgtaagt gtttgtcaag agtttagtgc
     2161 atgagtacgt ttgctaaaac aacggcatta aatggtattg caatgatcgt taagttaatt
     2221 gccgcattat tagctaataa atttgtcgca gtcatgttag gtcctcaagg actggctatt
     2281 tatgggcaat ttcaaagtat tctga
//
LOCUS       DAOQMB010000375         2304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_29849_length_2304_cov_17.373055, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000375 DAOQMB010000000
VERSION     DAOQMB010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29849_length_2304_cov_17.373055"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..>2304
                     /locus_tag="PLY05_10115"
     CDS             184..>2304
                     /locus_tag="PLY05_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03693.1"
                     /translation="MTNLKISLANNRLQRLELLDLLAHSKDFSLTLILAPAGCGKSTL
                     LQQWRQDNPQLSTATLSLHRRHHDPVVFLRQFHELLAKQFSHINLLSINAFNARLEQA
                     DTLAQALLESFAQIDHEFYLIIDDFHYATAPIIQELFALLLADLPNHIHLILATRSYP
                     DFSLSRLKLDDKLLVIDSHDLRLPPHQLNELCQVLQQPQLTKHETDELFRLTEGWLAG
                     IKLALLVRAKTGTLSTHNIQGSQPEIVDYFVNVVLNELSNDVREYLLASALFDKFDAK
                     LMQHILPHLDSKLILQRIIQKGLFINLIDEHQQSYRYHPLFQKSLIIYLQQHHSNSYV
                     EHLHNCAADYFLVHNDSESALYHSQHLQESDRFLQILETCCQTWFKEGQLLLILRWLD
                     KVKQEHRLAYPNLALLQLAALIFSRQFSQAKYLLEVIKQHFLISPNPALNDILFFLDK
                     VFNLFDRDNYEQVNDLHELDYQQCTHDIRVASPIFMARHYMLKGQCEEAIRQAHKGHV
                     LLKESGHEYMSSFTKVIIILSERELGHILIARQMTQEFFEQYIQQENTPCWINAATCM
                     AVSLYEQNRRDEAKSLCEKLIMAVDYACATELVFYVYVTLARLQGSQNPHRASQLLLQ
                     LRRILRHGTYNRFLNQLLAEELSHALRSNKINQIKHIVEDYDLVEHLQAGVWQQAPNH
                     YQETWVYGGIAAALYLRSKKQYDKA"
BASE COUNT          712 a          433 c          392 g          767 t
ORIGIN      
        1 gttataacac gcttttacgt cattttttgc ttggttagta tgttttttta ggccaaaccc
       61 gtttcattag ccccatcttt tttacatttc cccatacttt aggagggcta gtttttagaa
      121 tttagggtta taatctaagc aaataaaaat aacagctcat tttccgattc atggaacctc
      181 attatgacta atttaaagat cagtcttgca aacaatcgtt tgcaacgtct tgaattatta
      241 gacttattag cacatagcaa agactttagt ttgacactca ttttagctcc tgctggctgt
      301 ggaaagtcaa ccttactgca acaatggcga caagataatc cacaattaag cacagccacc
      361 ttaagtttgc atcgacgtca tcacgatcct gttgtttttt tacgccaatt tcatgagtta
      421 ctcgctaagc agtttagcca cattaattta ttatcaatta atgcttttaa tgctcgtttg
      481 gaacaagcag acactttagc acaagcgtta ttggagagtt ttgcgcaaat tgaccatgag
      541 ttttatttaa ttattgacga ctttcattat gcaactgccc ccatcattca agaattattt
      601 gcattattgt tagccgattt acctaatcat attcatctta ttttggccac gcgtagctat
      661 cctgattttt ccttaagtcg gctcaagcta gatgataaat tattggtgat tgatagccac
      721 gacttgcgct taccacccca tcaactcaat gagctatgcc aagttttgca gcagcctcaa
      781 ttaaccaaac atgaaactga tgagttgttt cgtttaaccg aaggttggtt agcaggaata
      841 aaattagctt tattggttag agccaaaact ggcacattaa gtacgcacaa tattcaaggc
      901 tcacaacccg aaattgtcga ttattttgtc aatgttgtct taaatgaatt gtctaatgac
      961 gtccgtgaat atttattagc tagtgcctta tttgataaat ttgatgccaa attaatgcag
     1021 catattttgc cgcatttaga tagcaaactg atattacaac gaattattca aaaaggcttg
     1081 tttatcaatc tcatagatga acatcaacaa agctatcgtt atcatcctct atttcaaaaa
     1141 agcctcatca tttatttaca acaacatcat tcaaatagct atgttgaaca tttacataat
     1201 tgtgccgcag attatttttt agttcataac gacagtgaat cagctttata tcactctcaa
     1261 catctacaag aatcggatag atttttgcaa attttagaaa catgttgtca aacatggttt
     1321 aaagaaggtc aacttttatt aattttgcgc tggttagata aagttaaaca agaacatcgc
     1381 ttagcgtatc ccaatttagc tttgttgcaa ttagcggcgt taattttttc tcgtcaattt
     1441 agccaagcta aatatttatt agaggtgata aaacaacatt ttttaatatc acccaaccct
     1501 gcactcaatg acatattatt ctttttagat aaagtcttta atttatttga ccgcgataat
     1561 tatgagcaag ttaatgattt gcatgagcta gactatcaac aatgtactca tgatattcgt
     1621 gttgcctcac ctatttttat ggctcgtcac tatatgctaa aaggacaatg tgaagaagcc
     1681 attcgccaag cacataaagg ccatgtgtta ctcaaagaat ctggccatga atatatgtct
     1741 agttttacta aagtgattat tattttatca gagcgtgagc ttggccatat tttaatcgcc
     1801 agacaaatga ctcaggagtt ttttgagcaa tatatacaac aagaaaatac cccctgttgg
     1861 attaatgcag cgacttgtat ggcggtgagt ctatatgagc aaaatcgtcg agatgaggcc
     1921 aaaagtttgt gcgaaaagct gattatggcg gttgattatg cctgtgcaac cgagttagtt
     1981 ttttatgttt atgtcaccct tgctcgttta caaggctcac aaaaccctca tcgtgcaagt
     2041 cagttattac tgcaattacg acgtattttg cgtcatggca cttacaatcg ttttttaaat
     2101 cagttattgg ctgaagaact tagtcatgcg ttgcgtagca ataaaattaa tcaaattaaa
     2161 catattgttg aagattatga tctagtcgaa catctacagg caggagtttg gcaacaagca
     2221 ccgaatcact atcaagaaac atgggtgtat ggtgggattg ccgctgcctt atacttacgc
     2281 agcaaaaaac aatatgacaa agcc
//
LOCUS       DAOQMB010000376         2298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30002_length_2298_cov_14.437361, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000376 DAOQMB010000000
VERSION     DAOQMB010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30002_length_2298_cov_14.437361"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PLY05_10120"
     CDS             complement(<1..1063)
                     /locus_tag="PLY05_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016145412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNC03694.1"
                     /translation="MDNLLDKAGSVRQGEELDITRIDQFLKSQLPSLQGEPRLEQFAG
                     GASNLTYLLAYADRDLILRRPPFGHKAKSAHDMLREAHIMTALKPVYPYVPSVLASSH
                     ADEVMDVDFYVMERIKGIIPRQNLPKSLNLNPEQTRQLCINVIDKMIELHQVDYRAAG
                     LSHLGKGEGYVKRQIDGWTDRYEKARTPDVGSFADVMAWLKAKMPSQDVATCLIHNDF
                     RFDNVVLNPDNPLEVIGVLDWEMATLGDPLMDLGNTLAYWVQADDDGVMQMMRRQPTH
                     LAGMLTRQEVIDYYGQKTGYQVDNFDFYSIYGLFRLAVIAQQIYYRFYHGQSKNTQFA
                     MFGQMVNYLEQRCLGLIANS"
     gene            complement(1069..2298)
                     /locus_tag="PLY05_10125"
     CDS             complement(1069..2298)
                     /locus_tag="PLY05_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC03695.1"
                     /translation="MDFSYSAKAQDYLARLQQFMRDEVYPAEAAYFQSLSHSPNHQDW
                     RQPEVMEHLKVKAKAAGLWNLFLPDEEYGAGLSNSDYAPLAEMMGTSFIASEVFNCNA
                     PDTGNMEVLVKYGSEQQKQQWLLPLLSGEIRSAFCMTEPDVASSDATNMQATAIIDGD
                     EVVINGRKWWSTGIGHPNCKILIFMGLTDPTAEKHRQHSMVLVPIDAQGVKVERMLPV
                     FSSFDEPSGHGEVSFTNVRVPISNIIAGAGRGFEIAQGRLGPGRIHHCMRAIGASERA
                     LTLLCQRAGTRVAFGKPIAKLGANPDIVANCRMAIEQARLLTLKAAWMMDTVGVKGAM
                     SEISQIKVVAPNVAQQVTDAAIQIHGGAGLSDDFPLAALYAYARVLRLADGPDEVHRA
                     LIAKLEYKKQIAKLAGA"
BASE COUNT          675 a          566 c          424 g          633 t
ORIGIN      
        1 tagagtttgc gattaaacct aaacaacgtt gctcaagata attgaccatt tggccaaaca
       61 tggcaaattg ggtatttttg ctttggccat gataaaaacg ataataaatt tgttgggcaa
      121 tcaccgctaa acggaacaag ccataaatac tataaaaatc aaaattatcg acttgataac
      181 ctgttttttg gccataataa tcaatcactt cttggcgcgt taacatacct gccaaatggg
      241 tgggctgacg acgcatcatt tgcatcaccc catcatcatc tgcctgcacc caatacgcta
      301 aagtgttgcc taaatccatt aaggggtcgc caagtgtggc catttcccaa tctaacacac
      361 caatcacttc taaaggatta tcaggattta gcacgacatt atcaaaacga aaatcattat
      421 gaatcaaaca agtagccaca tcttgactgg gcattttggc ttttaaccat gccatcacgt
      481 ccgcaaaact gccgacatcg ggcgtgcgtg ctttttcata acggtctgtc caaccatcaa
      541 tttgtcgttt gacatagcct tcacctttgc ccaaatggct taaacctgct gcgcgataat
      601 ccacttgatg caattcaatc attttatcaa tgacattaat gcacaactgg cgcgtttgct
      661 cagggtttaa attgagtgat ttaggcaagt tttgccgagg aataatgcct ttaatccgtt
      721 ccatgacata aaaatcgaca tccatcacct catctgcatg gctactcgcc aatacgcttg
      781 gcacataagg atagacaggt ttgagtgcgg tcataatgtg tgcttcacgc aacatatcat
      841 gtgccgactt agctttatgg ccaaacggtg gacgacgcaa aatcaagtca cgatcggcat
      901 acgctaataa ataggttaaa tttgacgcgc cacctgcaaa ctgttctaaa cgtggttcac
      961 cttgtaaaga cggcaactga ctttttaaaa attggtcaat acgggtaata tctaactctt
     1021 caccctgacg caccgaacct gctttgtcta aaagattgtc catactagtt atgctcctgc
     1081 caatttagcg atttgctttt tatattctaa tttggcaatc aaggctctat gcacttcatc
     1141 agggccatcc gctaaacgta atactcgtgc ataagcatat aatgccgcta aggggaaatc
     1201 atcggacaag cctgcgccac catgaatttg aattgccgcg tccgttactt gttgggcaac
     1261 attgggcgca accactttaa tttgggaaat ttcgctcatt gcccctttta cacccacggt
     1321 atccatcatc catgctgctt ttaacgttaa taatcgtgct tgctcaatcg ccatgcgaca
     1381 gttagcaaca atatctggat ttgcgcctaa tttagcgata ggcttaccaa aagccacccg
     1441 tgttcccgca cgctgacaca acaatgttaa agcacgttca gacgcgccaa tcgcacgcat
     1501 acaatgatga atacggcctg gccctaaacg cccttgtgca atttcaaaac cacgtcctgc
     1561 ccctgcaata atgttactaa tcggcacacg cacattagta aaggacacct caccatgacc
     1621 tgatggctca tcaaaactgg aaaaaacggg caacattctt tctactttga cgccttgagc
     1681 atcaatcggc actaatacca ttgaatgttg acgatgtttt tcggctgttg gatcggttaa
     1741 gcccataaaa atcaaaattt tgcagttggg atgaccaata cctgtactcc accacttgcg
     1801 gccattaatc accacttcat caccatcgat aatcgcggta gcttgcatat tagtggcatc
     1861 actcgaggca acatcaggtt cagtcataca aaatgccgaa cgaatctcac cgcttaataa
     1921 tggcaataac cattgttgtt tttgctgttc agaaccatat ttaaccaaga cttccatatt
     1981 gcctgtgtcg ggcgcattac agttaaatac ttcagaagca ataaaagacg tacccatcat
     2041 ttcggctaag ggtgcataat cactattact taaacctgca ccatattctt catcaggcaa
     2101 aaacaaattc cacaagccag cagccttcgc tttgactttt aagtgctcca tcacttcagg
     2161 ctgtcgccag tcttgatgat ttggactatg acttaaactt tgaaaatagg ccgcttcggc
     2221 aggataaacc tcgtcacgca taaactgttg caaacgtgcc agataatctt gggctttggc
     2281 agaataagaa aaatccat
//
LOCUS       DAOQMB010000377         2296 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30044_length_2296_cov_6.717537, whole genome shotgun sequence.
ACCESSION   DAOQMB010000377 DAOQMB010000000
VERSION     DAOQMB010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30044_length_2296_cov_6.717537"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1529)
                     /locus_tag="PLY05_10130"
     CDS             complement(72..1529)
                     /locus_tag="PLY05_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC03696.1"
                     /translation="MSGQKQRLLQALSHANNYQEWLAIAQELDQVDGYLDWRASDGSA
                     FFHENLIKEHIQKMQALRQSGDWQHLAVLLQESVYRHLGELNNPALYQYARSGTKYIV
                     GQYLDEVEQVMRTLSQQKIPQMSEQQKLALFEQAERVYGRPALMLSGGAAFGIYHVGV
                     TKALWEAQLLPQTIAGSSMGAIVAAAVCNRSDAELDIFYREQLSDIHRHAIRWRRPTD
                     IKKYKTALDETQIFEHIVTNVGSATFLEAYQRSGRVLNISVSPTRTHQKPRILNYLTA
                     PNVLIEYAAQASCAIPYMFPPVALQAKSSSGQKVPYMSTETWIDGTIHGDLPKERLAR
                     LHNVNQTIVSQANPHVIPFITHKKQRGVQAIGKHIISSLIHTTSAEFLDISKHLFQKT
                     PLSPVFNQAHAVAAQSYLGDINIQFPFSVKAYLKVIANPSPAGLVDYIRMGEQATWPQ
                     MAMIRDLTRISRVFPECIALIKQRILAETDENVPK"
     gene            complement(1816..2106)
                     /locus_tag="PLY05_10135"
     CDS             complement(1816..2106)
                     /locus_tag="PLY05_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03697.1"
                     /translation="MKHIIINMLCSFLILGCSKVKFEPFKIEFLENEYGYKMPIDLAG
                     KHNPEFIKCIFSKDDNADVIDVGYSKEVAFYVVNCPRTHERVYVFRTNTKRL"
BASE COUNT          674 a          446 c          446 g          730 t
ORIGIN      
        1 atttaagttc atgaaccaat tctgacatca agcgttgtca acttctagtg tctttacagg
       61 catttttata gtcattttgg gacattttca tcagtttcag ccaaaatacg ttgtttgatg
      121 agggcaatac attctggaaa aacacggcta attcgggtta agtctcgaat catggccatt
      181 tgtggccacg ttgcttgttc ccccatgcga atataatcga ccaaacccgc ggggctagga
      241 ttagcaatta cctttaagta ggctttaaca ctaaacggaa attgaatatt gatatcgccc
      301 aaataacttt gtgccgccac cgcatgggct tgattaaaga cagggcttaa gggtgttttt
      361 tgaaaaagat gtttagaaat atctagaaac tctgcactag tggtatgtat taacgaagaa
      421 ataatatgtt tgccaatggc ttgcacaccc cgttgttttt tatgagtaat aaaaggaatg
      481 acgtgtggat tagcttggct gacaatggtt tgattaacat tatgcaaacg tgccaaacgc
      541 tctttgggca aatcaccatg aatcgtgcca tcaatccatg tttcggtact catataaggc
      601 actttttgtc cagaagatga ttttgcttgt aaggcgacag gtggaaacat atacggaatt
      661 gcacaggatg cttgagcggc atattcaatc aagacattag gtgcggttaa ataatttaaa
      721 atacgtggct tttggtgagt tcgtgtgggt gataccgaaa tatttaacac ccgaccacta
      781 cgttgatacg cttccaaaaa cgttgcgctc ccaacattag tgacaatatg ttcaaatatc
      841 tgtgtttcat ctaaagcggt tttatatttt ttgatgtctg ttggtctgcg ccaacgaatc
      901 gcgtggcgat gaatatcgct taattgttca cgataaaaga tatccaactc ggcatcactg
      961 cgattgcaca ccgccgccgc gacaatcgcg cccatacttg agccagcaat ggtttggggt
     1021 aataattgtg cttcccataa ggctttcgta acgccaacat gataaatacc aaaggctgcc
     1081 ccaccactca acattaaggc aggacggcca taaacacgtt cggcttgttc aaataaggcg
     1141 agtttttgtt gttcggacat ttggggaatt ttttgttggc tcaaggttcg catcacttgt
     1201 tcgacttcat ccaaatattg gccaactatg tattttgtgc cagagcgtgc atattgatat
     1261 aaggcaggat tgtttaattc gcccaaatgt cgatacacgc tttcttgtaa taacaccgct
     1321 aaatgttgcc aatcacctga ctggcgtaag gcttgcattt tttggatatg ttctttaatc
     1381 aaattttcat gaaaaaacgc cgatccgtca ctggcacgcc aatccaaata accatctact
     1441 tggtctaatt cttgggcaat cgctaaccat tcttgatagt tattggcgtg agataaggct
     1501 tgtaataagc gttgcttttg accagacatg attttttcct aaaaaacggt ggttattctt
     1561 aggattctaa accatttttt agtcagatta ttgattaaat atacacttgt attttaatta
     1621 ttacctaaaa actgaaaaga gatataaaac tggtttcgta atgtgggttg acgcaggaaa
     1681 ctgacaattg agatttatca tcaccgtggg tttcgtgcct caaccacact ttacaaaatt
     1741 ttaatcttgc aaaaatattt acacagtatg tcactaggtt gcatattgtc tacgatactg
     1801 cctagcttat gatagttaca gtcgcttggt atttgttcta aatacataaa ctctttcatg
     1861 cgttcgtgga caatttacca cataaaaagc tacttcttta gaatagccta catcaataac
     1921 atcagcattg tcgtctttgg agaaaataca ttttataaac tcaggattgt gtttacccgc
     1981 taaatcgatt ggcattttat agccatattc attttctaaa aattcgattt tgaatggttc
     2041 aaattttact tttgaacatc ctaatatcaa aaatgaacat aacatattaa ttataatatg
     2101 cttcataatc taggaagctt ccaagtttcg tagtgtgggt tgacgtagga aacccacaat
     2161 tgagatttat catcatcgtg ggtttcgttt ctcaaccaca cgctaagtaa taaaaggcga
     2221 ccacaagggt cgcctctacc gtcaaaccgc ttcttgtaat tgttgcaaaa tcgctggatt
     2281 ttcgagggta gaaata
//
LOCUS       DAOQMB010000378         2293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30126_length_2293_cov_2.895442, whole genome shotgun sequence.
ACCESSION   DAOQMB010000378 DAOQMB010000000
VERSION     DAOQMB010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30126_length_2293_cov_2.895442"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLY05_10140"
     CDS             complement(<1..1203)
                     /locus_tag="PLY05_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03698.1"
                     /translation="MSRTYANKTTAAIGEYFQSAQLRLKDGKNTKDVFDSVCHDNSVQ
                     TPKVLDTLLGKLGGFEQDVFDSVVHGMNVYEKAHGCEAPADVIEQALHNAVASQMTLG
                     ELNIRLDSATESHIDAYSLQPNRAIVSIMSAMAEAIPFAGYVPADIGSNEAKSLILHN
                     RAKSNWGGYAANGAIDGTFNGEPYMMPNRLIALTTANQTAYTGTARAQYQAGSRLVVD
                     GASAGLKLMAGRTQILVNGFVVAKDMRGSSLGGTTAVSGSTVIGVTEYTITGTVNNDT
                     GAIALTFSPALPNNTSVVAEVIADFERDPDTIPSFGFEVESFSYYAAGHRAMTELTMD
                     SQSQVRNEMNLDPASNSLFSLRTQLTNEQHYHALQLGYLISGNNSLTHDFNLAAQFAA
                     KSISDIFAD"
     gene            complement(1348..>2293)
                     /locus_tag="PLY05_10145"
     CDS             complement(1348..>2293)
                     /locus_tag="PLY05_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03699.1"
                     /translation="SEDVPQFKSGAESDTGVVEALGGKFDRTGVAPIQVWERTDGRLE
                     IISGRHRTDLARRSGEKTIPAQVHKESEGFTAKQAMMLDAELNIRDGQGKVKDYVDYF
                     THSEISEDEAERRGLIARSIGQRAFAIASKGSEELITLHRNDMISDQAAAEIADIAPN
                     NSAMQAVGLRVLQDNKPLNNALNTIKAVMALTREKGQEPDTFDLFGFDDSALKEAEAM
                     AKIASRKQRQIAEQLNAIKGAVKNPKLAAKHGVVVENEADALAKVKTMTAQKARWDNW
                     SSYADLLAEVRKELNDKLDSLDYEDDDYYWFCQEELAA"
BASE COUNT          618 a          518 c          477 g          680 t
ORIGIN      
        1 atcggcaaaa atatcgctaa tgctttttgc agcaaattgg gcggctaaat taaagtcatg
       61 cgtcaagctg ttattgcctg aaattaaata acctaactgc aaagcgtgat aatgttgctc
      121 attggttaat tgagtacgca agctaaacaa gctgttagag gctgggtcta ggttcatctc
      181 attgcgtact tggctttgag aatccatagt caattcagtc atagcgcggt gtcctgcggc
      241 ataataactg aaagattcga cttcaaaacc aaaagaggga atagtgtctg ggtcacgctc
      301 aaaatcagca atcacttcgg caacaacact ggtattgttg ggcaaagctg ggctaaaggt
      361 taaagcgatt gcgcctgtat cgttattgac tgtacctgta atggtgtatt cggtaacgcc
      421 gataacggta gagccactga cggcggttgt accacccaaa gatgagccac gcatatcttt
      481 agcaacaaca aaaccgttga ctaaaatctg tgtgcggcct gccatgagtt ttaagcctgc
      541 gctagcacca tcaacgacta aacgactgcc tgcttggtat tgtgcgcgtg ctgtgcctgt
      601 ataagcggtt tggttggctg tggttaaagc aatcaaacgg ttgggcatca tataaggctc
      661 accgttgaat gtgccgtcaa ttgcgccatt agctgcataa ccaccccaat tagacttggc
      721 gcgattgtgt aaaattaacg atttcgcttc attagagcca atatcggctg ggacatatcc
      781 tgcaaacgga attgcctcag ccatagccga cataattgac acaatggcgc gattaggttg
      841 caagctgtaa gcgtcaatat gcgattcggt agcagaatcc aagcgaatgt ttaactcacc
      901 taaagtcatt tgtgatgcaa cagcattgtg taaggcttgt tcgataacat cggctggagc
      961 ttcgcaacca tgcgcttttt catacacatt cataccgtgt acgacagaat caaacacatc
     1021 ttgttcaaaa ccgcccaatt tgcctaataa cgtatctaac actttaggcg tttgtacgct
     1081 gttatcgtgg caaacggaat caaatacatc tttggtgttt ttaccgtctt ttaaacgtaa
     1141 ctgtgcggat tgaaagtatt cgccaatagc agcagttgtt ttgtttgcat aagtacgaga
     1201 catgaatcta tcctctatca aaaaaaggga tttgttttag atagaaagat tgtgacacaa
     1261 aaaaataggt acttttttga gtggttacat gattgaaagg cgtaaaaaaa gcccactttt
     1321 tagggtgggc aaactcgcta agcaaaacta ggcggctaac tcctcttggc aaaaccaata
     1381 ataatcgtca tcttcgtaat ctaacgaatc taatttgtcg tttaactctt tgcggacttc
     1441 tgccaataaa tcggcataac tcgaccagtt atcccaacgt gctttttgtg cggtcatggt
     1501 tttaactttg gctaaagcgt ctgcttcatt ttcaactact accccatgtt tagcagccaa
     1561 tttaggattt ttaaccgccc ccttaatcgc atttaactgt tctgctattt gtctttgttt
     1621 acgactggca attttggcca ttgcttcggc ttcttttaac gcgctatcgt caaagccaaa
     1681 caagtcaaaa gtatcaggtt cttgtccttt ttcgcgtgtt aaagccataa ctgccttaat
     1741 agtattaagt gcattgttta atggtttgtt gtcttgcaac acgcgcaaac caactgcttg
     1801 catcgcactg ttattgggtg caatatcggc aatctctgcg gctgcttggt cactaatcat
     1861 atcgttacgg tgtaaggtga ttaactcctc actgccttta ctagcaatag caaaagcacg
     1921 ttgaccaatg ctacgcgcta tcagtcctct acgctcggct tcatcttcgc taatttcgct
     1981 atgggtaaag taatcaacat aatcttttac cttgccttga ccatcacgga tatttaactc
     2041 ggcatctagc atcatggctt gcttagcggt aaagccctct gattctttgt gtacttgggc
     2101 tggaattgtc ttttcaccac ttctacgcgc caagtcggta cggtgacgac ctgagataat
     2161 ctctaaacgt ccgtcagtac gttcccatac ttgaataggt gctacgccag tacggtcaaa
     2221 cttgccacct aaagcctcta ccacacctgt atcactttct gcaccacttt taaactgtgg
     2281 cacgtcttcg gat
//
LOCUS       DAOQMB010000379         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30183_length_2290_cov_8.943177, whole genome shotgun sequence.
ACCESSION   DAOQMB010000379 DAOQMB010000000
VERSION     DAOQMB010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30183_length_2290_cov_8.943177"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="PLY05_10150"
     CDS             complement(<1..1107)
                     /locus_tag="PLY05_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03700.1"
                     /translation="MDYILAAKLRIPTSPVQLVNRPRLISAQLSPITLICAPAGYGKT
                     VLASSWVNSLDMRCAWLSLDHTDNDTTQFMMHLVSAIQSQFPDFANQSHHLSSSNQLP
                     AISGLTRALLNDLGQLTEPLCLVLDDLHCLHEPFLHDALAFMIERLPPQLHLILTSRT
                     IPPFSLARLRAQRQLTEIYTQDLRFSVEEVQQFCNQSMQLKLTPDLVKSLETRTEGWI
                     VGLQLAALSLSNTSNKSMFIKNFAGDDRHITDFLMDEVLNQLSDELQKFLLETSLLGQ
                     FNASLCDSLRQTNNSRQLINELEKQNLFISCLDHKRGWYRYHHLFASLLKSRLEELYS
                     PDDITTLHQRASLWFTQHHLLNEAIQHSMNAQDYT"
     gene            complement(1571..>2290)
                     /locus_tag="PLY05_10155"
     CDS             complement(1571..>2290)
                     /locus_tag="PLY05_10155"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03701.1"
                     /translation="RGEQLIKEMQDLKAIYGYSKFNLIGHSQGSPTSRYAAAVRPDLV
                     ASVTSVNGVNKGSRVADVVRKVAPAGTVTEAVFKTVADGLAGFLTFVYGTPTLDQSSI
                     NALNSLTTPGLTTFNSKYPAGVPSACGEGAYSQNYTKNGVTHKVNYYSWGGVSQLTNV
                     LDPLDAGIGLLSAAFLFSGEKNDGLVGECSQRLGKVVRSNYWNNHLDAINGFFGIVNL
                     FETNPKTIYRAHANRLQTAGL"
BASE COUNT          734 a          397 c          469 g          690 t
ORIGIN      
        1 tgtgtaatct tgagcattca tactatgttg aatggcttcg tttaataaat gatgttgtgt
       61 aaaccacaaa ctagcacgtt gatgtaaggt ggtgatgtca tctggactat atagttcttc
      121 taggcgactt tttaatagtg aagcaaataa atgatgataa cgataccagc cacgtttatg
      181 atctaaacag ctaataaata agttttgttt ttctaattca ttaattagct gacgactatt
      241 gtttgtctgg cgtaagctat cacacaaact ggcattaaat tgccctaaca aactggtttc
      301 taataaaaat ttttgcagct catctgagag ttgatttaac acttcatcca tcaaaaaatc
      361 agtaatgtgc cgatcatcac ctgcaaaatt tttaataaac atagacttat tgctggtatt
      421 gcttaacgaa agtgcagcaa gttgtaaacc aacaatccag ccttcagtgc gtgtttctaa
      481 gctttttact aaatcgggcg ttaattttag ttgcattgat tgattgcaaa attgctgaac
      541 ttcttcaaca ctaaaacgta agtcttgggt ataaatttcg gtgagttgcc tttgagcgcg
      601 taagcgtgcc aaagaaaacg gcggtattgt tcggctggtg aggattaggt gtagttgtgg
      661 tggtaagcgt tctatcataa atgctaaagc atcatgcaaa aatggctcat gtaaacaatg
      721 taaatcgtct aatactaaac ataatggctc agtaagttgt cccaaatcgt ttaataaagc
      781 gcgtgttaag cctgaaatag caggtaattg attgctacta gataaatgat ggctttggtt
      841 agcaaaatca ggaaattgac tttgaatggc actgactaag tgcatcataa attgagtggt
      901 atcattatct gtatggtcta gtgataacca tgcacaacgc atatcaagtg agttgaccca
      961 agagctagct aatacggttt taccatagcc agcaggagcg caaatcagcg taatagggct
     1021 aagctgtgca gatattagtc gaggtcggtt gactaattgc acagggcttg taggtatgcg
     1081 caatttagca gcaaggatat aatccatcgt cttaaccatt gtggtgtgat gagggcttac
     1141 agcttggctt atggcaaatt gagtcgttta gataagcgtt aatcaatagc gtttaagctt
     1201 accagccgat tgtaagacct gttattgttg ttgtctttgc gtattttatt gacgatagat
     1261 gactaattat tgataaaaaa tgacaatgtt gtcggatgag tagaaaataa gctagacaga
     1321 ggttaaacaa agaaaaacta gtagtcttaa agacgaggaa aacagtcaca agggacgagg
     1381 actgagtttg atgttgttgt gttgacgcaa caacatcaca agacttagta gcaaaaaaat
     1441 tattaacagc ttttgactta gatatttgac cgaaattagg ttctaagtca aaagtcatta
     1501 acaaggtata tttgcaaaaa taaataatac aagttaaata aataaccgtc ttgtaacatt
     1561 atcgtattaa ttacaagcca gcagtttgta aacggttggc atgagcgcga taaattgttt
     1621 ttggattggt ttcaaataag ttaacaatac caaagaaacc gttaatagca tctaaatggt
     1681 tgttccaata gttagagcga accactttac ccaaacgctg tgaacactca cctaccaaac
     1741 cgtcattttt ttcgccacta aataagaagg cggctgataa caaaccaata cctgcatcta
     1801 aggggtcaag cacattggta agttggctga caccacccca agaatagtaa ttaactttgt
     1861 gtgttacacc gtttttagtg tagttttggc tataagcacc ttcgccacaa gcactaggta
     1921 cacctgctgg atatttgcta ttaaaagtag ttaaaccagg agttgtgagg ctatttaaag
     1981 cgttaattga gctttgatct aaagtcggag tgccataaac aaaagttaaa aagcctgcta
     2041 agccatcggc aactgtttta aagaccgctt ctgtgactgt acccgctgga gcaactttac
     2101 gaactacgtc tgctactcgt gagcctttat taacaccatt gacacttgtt acagaagcaa
     2161 cgaggtctgg acgaacagca gcagcataac gtgaagttgg gctgccttgg ctatgaccaa
     2221 ttaagttaaa tttgctataa ccataaattg cttttaggtc ttgcatttcc ttaattaatt
     2281 gttcgccacg
//
LOCUS       DAOQMB010000380         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30359_length_2283_cov_19.048923, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000380 DAOQMB010000000
VERSION     DAOQMB010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30359_length_2283_cov_19.048923"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..273)
                     /gene="rpsO"
                     /locus_tag="PLY05_10160"
     CDS             complement(4..273)
                     /gene="rpsO"
                     /locus_tag="PLY05_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNC03702.1"
                     /translation="MALTVAQKQAIMAKFARGEADTGSPEVQVALLSAQIDDLQAHFK
                     AHKHDHHSRRGLIRMVNQRRSLLDYLNRKDNARYAALIQALGLRR"
     gene            complement(549..1481)
                     /gene="truB"
                     /locus_tag="PLY05_10165"
     CDS             complement(549..1481)
                     /gene="truB"
                     /locus_tag="PLY05_10165"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNC03703.1"
                     /translation="MTKQPQIKRVRRPIDGILLLDKPQGMTSNYALQRVKHLFNAEKA
                     GHTGSLDPLATGLLPICLGEATKYSQHLLDADKVYRTRMRLGQKTTTADAEGEVIEEK
                     SVPTLNAEQIEQVLARFRGTIQQVPSMYSALKKDGKPLYELARKGIEVAREPRTVQIY
                     RLELLNVEPLYWELEVACSKGTYIRNLVEDIGDVLGCGAHVAELRRLASGCFQLDEHL
                     TLESLQQIADEQGLAGLDELLLAPWAAIADKPRVTLSDNASYYILHGNPVRVNNLPIN
                     EDVLIFDAQNRFLGVGYMNDDGLLAPRRLLKTSY"
     gene            complement(1493..1876)
                     /gene="rbfA"
                     /locus_tag="PLY05_10170"
     CDS             complement(1493..1876)
                     /gene="rbfA"
                     /locus_tag="PLY05_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004978763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNC03704.1"
                     /translation="MSQRMQRLADQIQRELADLIRKDLKDPRLGMITISAVKVSKDMG
                     YADVYVTVLGKDLTADYAPSLEVLNHAAGFLRAALGKSLQIRMIPRLRFHYDEVVMRG
                     NHMAALIHQAVSADEANHPNNEPSE"
     gene            complement(1954..>2283)
                     /locus_tag="PLY05_10175"
     CDS             complement(1954..>2283)
                     /locus_tag="PLY05_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC03705.1"
                     /translation="AMSGLLTPERRETIVGVAQVREVFHSSRFGAAAGCMVLEGIVYR
                     NKPIRVLRDEVVIFQGELESLRRFKDDMQEVRHGMECGMAVKGYNDIRVGDKIEVYDV
                     QHVQRTL"
BASE COUNT          683 a          526 c          422 g          652 t
ORIGIN      
        1 gtcttaacga cgtaaaccta atgcttggat caatgcagca taacgagcat tgtctttgcg
       61 gtttaagtag tctaacaagc tacgacgttg gttaaccata cggatcaaac cacgacgaga
      121 atgatggtcg tgtttgtgtg ctttaaagtg agcttgtaaa tcgtcgattt gagcagacaa
      181 taaagcaact tgaacttctg gggaaccagt gtcagcttca ccacgggcaa atttagccat
      241 gatggcttgt ttttgggcaa ctgttaatgc cattttaaac tccacgagat gcgtcagtga
      301 tcacgtttag ccgtgcatct ctacagctaa cgtcagaaaa tctcttactt agacccaata
      361 agctcatgag ccacagatac caagtatcga agtcgggtat tttaacgatt aacgcaacat
      421 tacgctatgg attttatgta gagaaatcgg gttcttgaca gtaacaaatg tcactatctt
      481 gagttatttg ataaatttgc tgaaaatctg ttcagtgatg caactatgca ctgctgaaca
      541 gattaaaatt aatacgatgt ttttaacaat cgtcttggtg ctagtaaacc atcatcgttc
      601 atataaccta cgcccaagaa acgattctgt gcatcaaaaa tcaacacatc ttcattaatt
      661 ggtaagttat ttacgcgaac agggttgcca tgcaaaatat aataactggc attgtcactt
      721 aaggtgactc ggggcttatc ggcaatggcc gcccacggag ctaagagtag ctcatctaat
      781 cctgccaaac cttgctcatc agcaatttgc tgcaaagatt ctaatgttaa atgctcatct
      841 aactgaaaac agccactggc caaacgacgc aattctgcaa catgtgcacc acaacccaac
      901 acatcgccaa tatcttctac caagttgcga atataagtgc ctttactaca ggctacttct
      961 aattcccaat ataatggctc aacgttgagt agttctagac gataaatctg cacagtgcgt
     1021 ggttcacggg caacttctat gcctttacga gcaagctcat ataagggttt gccatctttt
     1081 tttaatgcag aatacattga aggcacttgt tggattgtgc cacgaaaacg cgctaatact
     1141 tgttcaattt gctcagcatt gagtgtgggc actgattttt cttcaatcac ctcaccttct
     1201 gcatcagccg tggtcgtctt ttgtcccaaa cgcatccgtg ttcgatacac tttatcggca
     1261 tctaacaaat gttgagagta ttttgtggct tcacctaaac aaatgggcaa caaccccgtt
     1321 gctaaaggat ctaaactacc tgtatgcccc gctttttctg cattaaataa gtgtttaaca
     1381 cgttgtaagg cataattaga ggtcatgcct tgtggcttat ctaacaacaa aatgccatca
     1441 attgggcgac gtacacgttt gatttgtggt tgtttagtca taaatgccaa acttattccg
     1501 acggctcatt attaggatga ttggcttcat cagcactcac tgcttgatga attaatgcgg
     1561 ccatgtgatt accacgcata accacttcat cataatggaa acgtaaacgt ggaatcatcc
     1621 gaatctgtag acttttgcct aaagcggcac gcaaaaagcc tgctgcatga ttcaacacct
     1681 ctaaactggg tgcataatca gctgttaaat ctttacccaa aaccgtgaca tagacatcgg
     1741 cataacccat atctttactg actttcaccg cagaaatggt aatcatccct aaacgcggat
     1801 cttttaagtc tttgcgaatt aaatcagcca attcacgctg aatttgatcc gctaaacgtt
     1861 gcattcgttg actcactaat gcctcgacta aatcattaca tcaagccact ctaaccctca
     1921 acgtatttga ggattagaga aacttatcaa atattacaaa gtacgttgta cgtgttgtac
     1981 gtcataaact tcgattttgt cgccaacacg gatgtcgtta tagcctttaa ccgccatacc
     2041 acattccata ccatgacgta cttcttgcat atcatcttta aagcggcgta aagattccaa
     2101 ttcaccttgg aaaataacca cttcatcacg caagacacga atcggtttat tacgataaac
     2161 aataccttca agtaccatac aacctgctgc tgcaccaaaa cgagaagagt ggaacacttc
     2221 acgcacttga gctacgccaa caatcgtttc acgacgttct ggcgttaaca agccagacat
     2281 agc
//
LOCUS       DAOQMB010000381         2280 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30437_length_2280_cov_7.105169, whole genome shotgun sequence.
ACCESSION   DAOQMB010000381 DAOQMB010000000
VERSION     DAOQMB010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30437_length_2280_cov_7.105169"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..934)
                     /locus_tag="PLY05_10180"
     CDS             complement(56..934)
                     /locus_tag="PLY05_10180"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC03706.1"
                     /translation="MNDKNQLFQHAQARMRALAYQLLGSIHDADDVLQEAWIRWYKID
                     NPAFYHQGYLHRIVVNLCLDLLRQRKSQQKQYIGWWLPEPDMAEDNFVEELINIEDQY
                     QQASFAFLHVLEQLNPVERAVFVLRELFDSEFDKIAEYLSKTSSNCRQIYSRAKRKLK
                     DTPARHETSADQHQQLLMSFLMACQQGNEQALQQLLTRDAILYSDGGGNVKAALRPIY
                     SAAKTIRLLLALARKQSSEAQWQLQLINGRLAAVSRLNGQLQSVMTICANQEGIKEVF
                     IMLNPEKLNIQQKTAS"
     gene            complement(1084..>2280)
                     /locus_tag="PLY05_10185"
     CDS             complement(1084..>2280)
                     /locus_tag="PLY05_10185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC03707.1"
                     /translation="IEDTTMTNTTQPTVIILGASYAGLMACARLLHSRQKIQIMVIDA
                     KTHFEQKIRLHETLVGDETRRLALAPLLKKRGVTFIQAKVNGLRPDTQQVLLEDGQRL
                     NYDYLIYALGSVPSQKIHGSEQHAISMASCDQLQHTHLQLQQLAQQQGHIVVLGAGLT
                     AIEVCTELATRFSGLKLSLISKQFPSFHYHPHAQQHLTKFFAQQQIDIIADHVDHIDQ
                     KQLHTKQQQIIPFDLCIDCTGLKANPLAQQSQLKVDELGRLYVDEYLRLLGSETIFVV
                     GDAARVTVNQQILRMSCATAMPIGAHAGTNIVRLLAKKPLVAFNFAYLAQFLSLGRKN
                     GLMQMVDEQDQARSQISTGFWAARYKEWICKMTINMIYWELRTGLKLYFWPKQSSLTT
                     YQLPNN"
BASE COUNT          677 a          424 c          447 g          732 t
ORIGIN      
        1 ttaaccaata aacctttaga tattaagcaa attggtcaac aaattgcgca acagtttaag
       61 atgcagtttt ttgctgaatg ttaagttttt caggattaag cataataaac acttctttga
      121 taccttcttg attggcacaa atagtcatga ccgattgtaa ttgaccattt aaacgagata
      181 ctgccgcgag tcgtccatta atcaattgca attgccattg tgcctcgctg ctttgttttc
      241 tggctaatgc taacaataac cgaatcgttt ttgcggcact ataaatgggt cgtaaggctg
      301 ctttgacatt acctccgcca tctgaatata aaattgcatc tcgggttaac agttgttgta
      361 gggcttgttc atttccttgt tggcaagcca ttaaaaagct cattaataat tgttgatgtt
      421 gatcagcaga tgtttcatga cgagcagggg tatctttgag tttacgtttg gcacggctat
      481 aaatttgtcg acaattactg gatgttttgc ttaagtattc agcaattttg tcaaactcac
      541 tatcaaataa ttcccgtaac acaaaaacgg cacgttccac agggttgagt tgttctaaaa
      601 catgcaaaaa ggcaaatgag gcttgttgat attgatcttc aatattgatt aattcttcaa
      661 caaaattgtc ttcggccatg tctggctcag gtagccacca accaatatat tgtttttgtt
      721 ggcttttacg ttgtcgtagc agatcaaggc ataaatttac gacaatacga tgcaaatagc
      781 cttgatgata aaaagcaggg ttgtcaattt tataccaacg tatccatgct tcttgtaaaa
      841 catcatctgc atcatgaata gagcctaata attgataggc taatgcgcgc atacgtgctt
      901 gtgcatgttg aaacagttga tttttatcat tcatatcatg ctcctatggg tgttatgatt
      961 gacaactttg tgtaaatgag gacttacgca tttcaccttt aggcgcgggt tttgtgagca
     1021 taaaggtgtt aaaaacaact caaatgtgaa caaataagca ataaccacgt aagtcctaaa
     1081 atgttaatta tttggtaatt gataggttgt tagtgaagat tgtttcggcc aaaaatagag
     1141 ttttaagcca gtcctcaact cccaataaat catgttgata gtcattttgc aaatccattc
     1201 tttgtaacgc gctgcccaaa agccagtgct aatttgcgag cgagcttgat cttgctcatc
     1261 gaccatttgc attaagccat ttttgcgtcc caaactcaaa aattgggcaa gataggcaaa
     1321 attaaaggcg actaagggtt ttttggctaa tagccgcaca atatttgtac cagcatgtgc
     1381 accaataggc attgcggttg cacaagacat gcgcaatatt tgttggttaa ccgtcactcg
     1441 tgctgcatca ccaaccacaa aaatagtctc actgcctaat aaccgtaaat attcatcaac
     1501 atacaaacgc cccaactcat caactttcag ttggctttgt tgtgccaatg gattggcttt
     1561 taaccctgta caatcaatac ataaatcaaa aggaatgatt tgttgttgtt tggtgtgtag
     1621 ttgtttttgg tcaatatgat cgacatgatc tgcaataata tcaatttgtt gttgggcaaa
     1681 aaacttggtt aaatgttgtt gtgcatgagg atgatagtga aaagaaggaa attgtttgct
     1741 gattaagctt agttttagcc cagaaaaacg tgtggccaat tctgtacaaa cttcaattgc
     1801 ggttaaacct gcaccaagaa ccacaatatg accttgttgt tgtgctaatt gctgtaattg
     1861 taggtgagtg tgttgcaatt gatcacagct agccatactg attgcatgtt gttcactgcc
     1921 atgaattttt tggctaggca cactgcctaa agcataaatc aaataatcat aatttaaacg
     1981 ttggccatcc tctaacagta cctgttgagt atctggacgt aggccattaa cttttgcttg
     2041 aataaatgta accccacgtt tttttagcaa cggagcaaga gcaagacggc gtgtctcatc
     2101 accaactaag gtttcatgta agcgaatttt ttgttcaaaa tgcgttttgg catcaattac
     2161 catgatttgt attttttgac ggctatgtaa aaggcgagca caagccatta agcctgcata
     2221 actagcccct aaaatgatga cagttggctg tgtggtatta gtcatagtgg tatcctcgat
//
LOCUS       DAOQMB010000382         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30512_length_2277_cov_12.157066, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000382 DAOQMB010000000
VERSION     DAOQMB010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30512_length_2277_cov_12.157066"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1986)
                     /locus_tag="PLY05_10190"
     CDS             complement(148..1986)
                     /locus_tag="PLY05_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03708.1"
                     /translation="MMINANYLFTQTALAVGTQGTTDVLLRFNSLSTNTTQRQLNIAL
                     VIDRSGSMAGSSLKYALDAAQNVIDQLSPTDIISVVAFDDVISTIISPQHAINKQDLK
                     QRIRQIRAGGTTNLSGGWLEGCKHVQTHYNPTAINRVLLLTDGQANEGITDKRILTNT
                     AAKKAEQGIITTTLGFGANFEEDLLISMAKAAQGNFYFIQSKDEASQVFSIELESLKS
                     VVAQNLTATLTPIGNVSIEQCLTSHHQEKIGNTMTISLGDVYANEDKLLGLTLNLPCQ
                     EHIGLNSLLSVTFSADIVNHHHIEQVHGELTVNLPIVSVEEAAIAPSTGVRIDIARLK
                     IACAKDTALRLADQKQFVPAQQTLISLISQLQQQGLHEHFEIAEEIEQLNYFATRIAN
                     RNLSNETRKELRDQSFQGFHRNRMELNTRGIETSPEITQLKLVSDIGTGIELICVREH
                     GKLRIKINNNLFDSSINVQFPRAIRAEGAKYVVEDLEPSADGSFYRVKGAIHRLVGAG
                     ETDIFQRPQRDSIVRTGKASKVSISFHDLEVADSVGDCILVQCVNDGSKLRARVVSDG
                     FDPTWNMRFPRSIREEGMLYVVDEVITGPDGKSYIACGKVKRFEQI"
     gene            complement(2177..>2277)
                     /locus_tag="PLY05_10195"
     CDS             complement(2177..>2277)
                     /locus_tag="PLY05_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNC03709.1"
                     /translation="LKSALALVDIRVLDHFIVGEYEVISFAERGLL"
BASE COUNT          681 a          444 c          444 g          708 t
ORIGIN      
        1 gcatgattgc tccttacggg ttaacagccc aaacttaatg taggggcaag gagtaataaa
       61 gcctttttaa gaggcttcgc tatacctttg cacagtatca ctatgcaaag ggggacagat
      121 tataaaagtt agcctgatga atagcaatta aatttgttca aaacgtttga cttttccaca
      181 agcaatataa gatttgccat caggccctgt aattacttcg tcaaccacat acaacatacc
      241 ttcctcacga atgctgcgtg gaaagcgcat attccatgtg gggtcaaaac catcagacac
      301 gacacgggca cggagtttgc tgccatcatt aacacattgc accaaaatac aatcacccac
      361 agagtctgca acttctaagt catgaaaact aatcgagact ttgctcgctt tgcctgttct
      421 aacaatggaa tcacgctgtg ggcgctgaaa aatatctgtt tcaccagcac caactaaacg
      481 atgaattgca cctttgacac ggtaaaaact accatccgca gaaggctcta ggtcttcaac
      541 tacatatttt gcgccttctg ctcgaatagc acgtgggaat tggacattga ttgatgagtc
      601 aaaaagatta ttattgattt tgatgcgtaa cttaccatgt tctcgcacac aaatcagttc
      661 aatacccgtt ccaatatcag aaactaattt gagttgggta atttctgggc ttgtttcaat
      721 gccacgagta tttaactcca tacgattacg gtggaaacct tggaacgatt ggtcacgtaa
      781 ttctttgcgg gtttcattgg ataaattacg attggcaata cgagtcgcaa aataattgag
      841 ttgttcaatt tcttcggcaa tttcaaagtg ctcatgtaaa ccttgttgtt gtaattgact
      901 aattaggcta attaaggtct gttgagcagg cacaaattgt ttttggtcgg caagtcgtag
      961 agcagtatct tttgcgcaag caatttttaa gcgagcaata tcaatacgaa cacctgtgga
     1021 tggggcaata gcagcttctt caacacttac aatcggtagg ttgacagtta actcaccatg
     1081 aacttgctca atatgatgat gattgacaat atctgcactg aatgtaactg agagtaaaga
     1141 attaagcccg atgtgttctt ggcatggtaa gtttaaggtt aaccctaata atttatcttc
     1201 attggcatac acatcaccaa gactaatggt catggtattg ccaatttttt cttgatggtg
     1261 ggatgttaaa cattgctcaa tgctgacgtt gccaatgggg gttaatgttg cagttaagtt
     1321 ttgagcaaca acagatttta aggactctaa ttcaatacta aaaacttggc tagcttcgtc
     1381 tttgctttga ataaaataaa aattaccttg agctgcttta gccatactaa ttaataagtc
     1441 ttcttcaaaa ttcgcaccaa aacccaaggt agtggtaata atcccttgtt ccgctttttt
     1501 agccgcagta ttagtcaaaa tgcgtttatc tgtaatacct tcattggctt ggccatctgt
     1561 taataataaa acacgattaa tagcggttgg gttgtagtgt gtttgcacat gtttacagcc
     1621 ttctaaccat ccaccagaca aattggttgt accacctgca cgtatttggc gaatacgttg
     1681 ttttagatct tgtttattaa tggcatgttg gggggaaatg atcgtactaa tcacatcatc
     1741 aaaggccaca actgaaataa tgtctgtagg gcttaattga tcaatcacat tttgtgctgc
     1801 atccagagcg tatttcagac tagagccagc cattgaaccg ctacggtcaa tcaccagagc
     1861 aatatttaat tgacgttgag tggtattagt agaaagactg ttaaaacgta ataaaacatc
     1921 cgttgtgcct tgagttccta cagctaaagc agtttgggta aaaaggtaat ttgcgttaat
     1981 catcatatag ttccttattt aaattgtttg taggtttatc tgctgattgc ctatctgcaa
     2041 aaatcctaca tgacatcgtg ctaaatggtt tgaagatcct gttcggtgtt taatcctgtt
     2101 catggagtga tattttatat gttttatttt ttttaaatag gtatgttaac gactgttgcg
     2161 acattagttg tcgctgttac agcaggccac gttccgcaaa cgaaatcacc tcatactccc
     2221 caacaataaa atgatctaat acgcgaatat caactaaagc caaggctgat tttaagc
//
LOCUS       DAOQMB010000383         2272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30646_length_2272_cov_10.672982, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000383 DAOQMB010000000
VERSION     DAOQMB010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30646_length_2272_cov_10.672982"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="PLY05_10200"
     CDS             complement(<1..166)
                     /locus_tag="PLY05_10200"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNC03710.1"
                     /translation="MAQFTVTGEVDPFLHISLQQGEKIYCESNSMVMMEDTLDLKGKM
                     GGFGQALMRSF"
     gene            complement(186..1463)
                     /locus_tag="PLY05_10205"
     CDS             complement(186..1463)
                     /locus_tag="PLY05_10205"
                     /inference="COORDINATES: protein motif:HMM:NF021641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03711.1"
                     /translation="MDMIRGQKIKISDVTSGVFQIQVQLVAAMAIDVSCFGIDAQQKL
                     SDERYMIFFNQPNSPCGGVKLVNNQANQTSFQIDLSKIPSTIDKLVFTAAIDGQGTMA
                     QLQSSSLSVGSNCRFTFSGQDFAAERAIMIAELYRKEGVWRLAAVGQGFNGGLDALVT
                     HFGGEVAAPVSAPTPTIKPSLVKRVQLEKRIEQEAPQLVNLVKKAQISLEKSGLGEHR
                     AKVCLCLDISASMSDLYSKGQIQTFIERILALACRFDDDGEVDVFLFGQKSHHAPTID
                     LQNYQQYIRTLIKQYPLEGGTHYSPVMKMIRHHYLYDDSDLKSPARTSAELPVYVMFV
                     TDGATSNRKAAENQILYASFQPIFWQFMGIGRSGAFEFLEKLDDLKGRYVDNADFFSV
                     KSPQELTDEQLFARLMNEYPKWLLQAQQKQLLL"
     gene            complement(1520..2095)
                     /locus_tag="PLY05_10210"
     CDS             complement(1520..2095)
                     /locus_tag="PLY05_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019458110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC03712.1"
                     /translation="MAISLQKGGNVNLSKEAPTMTKMIVGLGWDVRATDGAAFDLDAV
                     AFVLGADGKVRKDTDFVFFNNKQNAEGSVAHGGDNRTGAGDGDDETIVVDLTKMPADA
                     EKVAVCVVIYDADSRRQNFGQVSRAYVRVINDAGQQEIARYDLSEDGSTEAAMIFGEV
                     YRYNGEWKFKAVGQGFKGGLGPLAASYGVNV"
BASE COUNT          719 a          503 c          351 g          699 t
ORIGIN      
        1 caaaactacg cattaaagct tgaccaaaac cacccatttt accttttaaa tctaaagtat
       61 cttccatcat caccatggaa ttagattcac aatagatttt ttcaccttgt tgcaaactaa
      121 tatgcaaaaa cggatcaacc tcacctgtga ctgtaaattg tgccatttat ttattaccta
      181 tctatttata acaataattg tttttgttgc gcctgtaata accatttggg gtattcattc
      241 attaaacgcg caaacaactg ttcatccgtt aattcttgag ggcttttaac actaaaaaaa
      301 tcagcattat caacataacg cccttttaaa tcatctagct tttctaaaaa ctcaaaagca
      361 cccgaacgtc ctatacccat aaattgccaa aaaataggct gaaatgaggc atataaaatt
      421 tgattttctg ctgctttacg attacttgtt gcaccatcag tcacaaacat cacatataca
      481 ggtaattcag ctgaagtacg agcgggcgat ttaagatcac tatcatcata caaataatga
      541 tgacgtatca ttttcatgac aggagaatag tgtgtaccac cttctaaggg atattgtttg
      601 attaaggtgc ggatatattg ctgataattt tgtaaatcaa tggttggtgc atgatgagac
      661 ttctgaccga ataaaaacac atctacttca ccatcatcat caaaacgaca ggctagtgcc
      721 aaaattcgct caataaatgt ttgtatttgt cctttactat ataaatcgct catcgaggca
      781 gaaatatcta aacataaaca aactttagct cgatgctcgc ctaggcctga tttttctaag
      841 gaaatttgtg cttttttaac aagatttact aattgaggcg cttcttgctc aatacgcttt
      901 tccaattgca cccgtttaac taaagagggt ttaatggttg gtgttggagc agatactggt
      961 gcagccactt ctccaccaaa atgcgttact aaagcatcta aaccgccatt aaagccttga
     1021 ccaactgctg ctagtcgcca tactccttct ttgcggtata gctcagcaat cataatcgct
     1081 cgttcagctg caaaatcttg accactaaag gtaaaacgac aatttgaacc cacagacaaa
     1141 gaacttgatt gcaattgcgc cattgttcct tgaccatcaa tcgccgcggt aaagactaac
     1201 ttatcaattg tggaaggaat tttactaaga tcaatttgaa aactggtttg attcgcttga
     1261 ttattaacta atttaacacc accacatggg ctattgggct gattaaaaaa aatcatatac
     1321 cgctcatcac tgagcttttg ttgggcatca atcccaaaac aactgacatc tattgccatt
     1381 gcagcaacta attgcacttg tatttgaaac acacccgacg tgacatcaga tatttttatt
     1441 ttttgcccac gaatcatatc catgatttta tcctaaaaac aaaaaggcga gacaacctcg
     1501 ccttttatca ttatcgtatt tatacgttaa cgccataact agcggctaaa ggtcctaaac
     1561 cacctttaaa accttgacca accgctttaa atttccactc accgttataa cgatatactt
     1621 caccaaaaat catcgcagct tctgtagaac cgtcttcaga taagtcataa cgagcaattt
     1681 cttgctgacc tgcatcatta atcacacgga cataagcacg agacacttga ccaaaatttt
     1741 gacgacggct gtctgcatca taaatcacca cacaaacagc gactttttct gcatccgcag
     1801 gcatttttgt taaatcaaca acaatcgttt cgtcatcgcc atcacctgca cctgtacggt
     1861 tatcaccgcc atgagcaact gagccttccg cattttgttt attgttaaaa aatacaaaat
     1921 cggtgtcttt gcgaacttta ccatcagcac ccaatacaaa agcaactgca tctaagtcaa
     1981 aggctgcacc atcagttgcg cgtacatccc aacctaaacc aacaatcatt ttagtcatcg
     2041 ttggggcttc tttgcttaag ttaacattgc cgcctttttg taagctaatt gccatcttta
     2101 tctccaaata atcatgtatt gatagttgaa gagaatatgg cgttttttat tgatattttc
     2161 aaaagagaat ttataggaga attgttaaga aaacatatta ttttttcttc cccccaataa
     2221 aaaacccttc tagttttgct agaagggttt ttttgattgg agtctggcga tg
//
LOCUS       DAOQMB010000384         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_30890_length_2262_cov_7.861803, whole genome shotgun sequence.
ACCESSION   DAOQMB010000384 DAOQMB010000000
VERSION     DAOQMB010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30890_length_2262_cov_7.861803"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PLY05_10215"
     CDS             <1..324
                     /locus_tag="PLY05_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004663679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03713.1"
                     /translation="DGSRLSVVGRYDLTQGALQFSDPIDDSIFGFDNMSGMIQFNNNI
                     VINKDSVAFKYAFEFNPDKTASKVFRIKDFNLYPPGTARAQRLGEMVITGGRYTAELN
                     IKPRN"
     gene            342..1994
                     /locus_tag="PLY05_10220"
     CDS             342..1994
                     /locus_tag="PLY05_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004669905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03714.1"
                     /translation="MNNKNIFKKTLIASALSVALTPIIALADMAELSDSEMSDEVGQA
                     LLSMGYTAPSGSGTGVTTSDYAYYKLGLEAKMEINLNIRNLQLGCGGRNGPGACDIDI
                     ENLSLSGPADGKTTDLGTPANNGIPLWTTAGGRPNSSAVLTNPFVEFAIKNPNTAATR
                     EIVGFRLSAEEILGHMSAGTVNDNVGGFSTGGGIKSFSGYLKVGNTPVKAATQEAIFG
                     TTPDQAIYGKITSLGNARTVHANVNKMTPGAPGFTAPGNSNANFVTSPLTGNKMWGIK
                     VPSVAVNFDFPQTSVVGNRMSQLNLVVRDVPVGNTINGAPNDGFGRIAIGRDSGGISM
                     VLDEDILGLVGTATFFMGANNASGTVAACGASYSAANCSYISNLKANVTVKQNFNLVH
                     NLPISSGGYLSLQREALRWPGSASAFKYDLTTKAITNVADNYVASPTELFNAGDIAQP
                     GWWLSFKDPLDFGALNPTTNIPMNDVLPQIATFITDYLSNNNIAVGVGDSLGALFGSP
                     LYKGLGDITLASNARAVMVLQNLLLDGNQQPVSNCWGGLKFC"
BASE COUNT          668 a          370 c          493 g          731 t
ORIGIN      
        1 gatggtagtc gtttatcagt agttggtcgt tatgatttaa cacaaggtgc attacagttt
       61 agcgacccta ttgatgactc gatttttggc tttgataaca tgtcgggtat gattcaattt
      121 aataataata ttgtgattaa taaagatagt gttgctttta aatatgcgtt tgaatttaac
      181 ccagataaaa cagcaagcaa agtttttcga attaaagatt ttaacttata tccacctggt
      241 actgctcgcg ctcaacgatt aggcgaaatg gtcattacag gtggccgtta cactgcagag
      301 ctgaatatta aaccacgcaa ctaagctaaa agggtaatgt catgaataac aaaaacatat
      361 ttaaaaagac gttaatcgct tcggcattgt ctgttgcgct aactccaata atagcattag
      421 cggatatggc cgaactaagc gatagtgaaa tgtctgacga agtaggtcag gctttattat
      481 caatgggcta cacagcgcct agtggttcag gaacgggtgt cacaacatct gactatgctt
      541 attataaatt gggtttagaa gccaaaatgg agatcaattt aaatattcgt aatttgcaat
      601 tgggttgtgg tggtcgcaat ggtcctggtg cttgcgatat tgatattgaa aacttatctt
      661 tgtctgggcc agcagatgga aaaactacag acttagggac tcctgcaaat aacggtatcc
      721 cactgtggac aactgctggt ggtaggccaa atagtagtgc agtattaaca aatccttttg
      781 tggagtttgc gatcaaaaat cctaatacag cagcaacccg agagattgtt ggttttagat
      841 tgagtgccga agaaatttta gggcatatgt cagcgggtac ggttaatgat aatgttggcg
      901 gtttttccac aggtggtggg ataaaatctt ttagtggtta tcttaaagtc ggtaacacac
      961 ctgttaaagc ggcaactcaa gaggcaattt ttggtacaac tccagaccaa gcaatttatg
     1021 gtaaaattac ttcgttgggt aatgctcgca cagtacatgc aaatgtaaat aaaatgactc
     1081 ctggtgcccc aggctttacg gcacctggta acagcaatgc aaattttgtc actagtccgc
     1141 taacgggaaa taaaatgtgg ggtattaaag taccatcagt tgctgttaat tttgattttc
     1201 ctcagacatc cgtggttgga aatcgtatgt ctcagttaaa cttggttgtg cgagatgtac
     1261 cagttggaaa taccatcaat ggagctccaa atgatggctt tggacgcatt gctattggtc
     1321 gtgatagcgg cggtattagt atggtattag atgaagatat attaggttta gtgggaactg
     1381 cgacgttttt tatgggagca aataatgcta gtgggaccgt ggccgcatgt ggtgcctctt
     1441 atagtgctgc taattgttca tatatttcaa atctaaaagc caatgttacc gttaaacaaa
     1501 attttaattt ggttcataac ttgccaatca gctcaggagg ttatttgtcc ttgcaaagag
     1561 aagctttgcg ttggccaggc agtgctagtg cgtttaaata tgatttaacc acgaaagcaa
     1621 ttacaaatgt ggcagataat tatgtggcat ctccaacaga gttgtttaac gctggagata
     1681 ttgctcaacc aggctggtgg ttatcgttta aagatccttt agattttggt gcattaaacc
     1741 caacaaccaa tattccaatg aatgatgttc tacctcaaat agcaactttt attactgatt
     1801 atttatcaaa taataatatc gctgttggag ttggcgattc tttaggagca ttatttggtt
     1861 cgcctttata taaaggttta ggcgatatca cacttgccag caatgcgcgt gcggtcatgg
     1921 tattacaaaa tctattgtta gatggtaacc agcagcctgt ttctaattgt tggggtggat
     1981 taaagttttg ttaatcaaag cggcgtgagc cgctttttta tgatctgttg attattagct
     2041 tatctaaatg gataagttaa tattttagca gtcttaaaag attattctaa aataaatacg
     2101 ataaatctaa aaaaacgtag attcactctt ttctaaaatc aaaagttccg tataatcttc
     2161 gcctttgttt attttattat tggcttgttt ttgacaagct aactttaagt tgctttatct
     2221 ttagaggatg tcatgcaagt ttctgttgaa aatgtttctg gc
//
LOCUS       DAOQMB010000385         2251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31163_length_2251_cov_17.880237, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000385 DAOQMB010000000
VERSION     DAOQMB010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31163_length_2251_cov_17.880237"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLY05_10225"
     CDS             complement(<1..947)
                     /locus_tag="PLY05_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03715.1"
                     /translation="MKLSHYLRLGVVCSVIGVQTLISGCSQVVKGGAHIALSFSESYI
                     VPPILQLNDAPMACASGESLTPLILATQGMGADPAKLAVFMYVSAGLCAEDRALENEL
                     RYLRASRANMIEEAQDARINQKRWAALAARRQQEAYNRFVERFEKEFRFKIGASCPTL
                     KEDFDKLVYLVGLASGMQAIVNDIAAQGVVGVPKDIAAIVERGMACLDNAEYWGVPMA
                     TRAAVWSLLPGASEGKPDPFTVMKDSTRVGERKGVRLAHAIYALAAQAKGDEALLRDA
                     LKTYGAANNMAVNPQYRLLDALGGMMVQTISDRYWTEKTG"
     gene            complement(1283..>2251)
                     /gene="pnp"
                     /locus_tag="PLY05_10230"
     CDS             complement(1283..>2251)
                     /gene="pnp"
                     /locus_tag="PLY05_10230"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNC03716.1"
                     /translation="ENFMLHYNFPAYSVGETGRESGPKRREIGHGRLARRGVQPMLPS
                     PEKFPYAVRVVSDITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGDRF
                     AVLSDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQARGGRLHI
                     LNAMNQVIANARPEVSIHAPTYTTLQINPEKIRDVIGKGGATIRAICEETKATIDIED
                     NGFVRIYGETKGATQAALAKVQAITAEVEVGAVYDGLVSKVVEFGAFVTIMPGTDGLV
                     HISQITNERVANVSDYLKEGQTVKVRVMDVDQRGRIKLSMKDVSNA"
BASE COUNT          696 a          518 c          379 g          658 t
ORIGIN      
        1 cctgtttttt ctgtccaata acggtcagaa atagtttgta ccatcatgcc gcccaaagca
       61 tccaataagc gatattgtgg gttaactgcc atattgttgg ctgcaccata agtttttaaa
      121 gcatcgcgca ataaagcttc atcaccttta gcttgtgctg ctaacgcata aatagcatgc
      181 gccaaacgca ctcctttacg ctcaccaaca cgggtagaat ctttcatgac agtaaatggg
      241 tctggtttac cttcgctagc accaggcaat aatgaccaaa cagcagcacg tgttgccatt
      301 ggtacgcccc agtattcagc attgtctaaa caagccatac cacgttcaac aatcgctgcg
      361 atgtcttttg gtacacccac aacaccttga gcagcaatat cgttaacaat agcttgcata
      421 ccagaggcta gacccaccaa ataaaccaac ttgtcaaagt cttcttttaa agttggacaa
      481 gaagcaccga ttttaaaacg aaattctttt tcaaaacgtt caacaaaacg attataggct
      541 tcttgttggc gacgtgcggc taatgcagcc caacgctttt ggttaatacg cgcatcttgt
      601 gcttcttcaa tcatattggc acgagaagca cgtaaataac gtaattcatt ttctaaagca
      661 cgatcttcag cacataaccc tgcagataca tacataaata cagctagctt agctgggtca
      721 gcacccatgc cttgtgtcgc taaaattaac ggtgttaatg attcacctga agcacaagcc
      781 attggtgcat catttaactg caaaattggt ggaacaatat aactttcact aaaagacaaa
      841 gcgatatgag caccgccctt aacaacttgt gagcaccctg atattaatgt ctgtacacca
      901 attacagaac aaaccacacc taaacgcagg tagtgtgata atttcatgcc ttatctccta
      961 gatttgtttt tttaatatat acacgttgat catggttggc ttgatgatta acgcaaaatt
     1021 aacgccaaga ttaataacat aaatcctagc atggttatta tttgaattgt ttatttgtca
     1081 aacaattact ttaaatatca cttaacctca aaaaacatca atcattattg aatgttctta
     1141 tacttaagcc atgataatgt cttattttta aaggtcgcaa tgattgttaa atttttatag
     1201 aacgtatcgc tctacttaaa cactctttcg ttgtaatcaa tgatacgagg gacaatcttg
     1261 acagatcatc cctcctctct ttttaagcgt tgcttacatc cttcatagat aacttaatac
     1321 gaccacgttg gtcaacatcc atgacacgaa ctttaacagt ttggccttct tttaagtaat
     1381 cactgacatt agcaacacgt tcgttggtga tttgggagat atgaaccaat ccatctgtac
     1441 ccggcataat ggtcacaaat gcaccaaact ctacaacttt agaaactaat ccatcgtaaa
     1501 ccgcgcctac ttctacttca gcagtaatcg cttgtacttt ggctaaagct gcttgtgttg
     1561 cgcctttagt ttcaccataa atacgcacaa agccgttgtc ttcaatatca atcgtggctt
     1621 tagtttcttc acagattgca cgaattgttg caccaccttt accaatcaca tcacgaattt
     1681 tttctggatt gatttgtaaa gttgtataag ttggtgcatg aatgcttact tctggacgag
     1741 cattagcaat cacttgattc atagcattta aaatatgcaa acgaccacca cgtgcttggt
     1801 ttaaagcaac ttccataatt tcttcggtga tgccttcaat tttaatatcc atttgtaaag
     1861 cagtaatacc attggcacta cctgctactt taaagtccat atcacctaaa tgatcttcat
     1921 cacctaaaat atctgaaagc acagcaaaac gatcgccttc tttaactaag cccatcgcaa
     1981 taccagcaac aggtgctttt aatggcacac cagcatccat taatgctaaa gaagcaccac
     2041 agaccgaagc catagaagaa gaaccattgg attcggtaat gtcagaaaca acacgcacag
     2101 cataagggaa tttttctggg gatggcaaca tgggttgtac accacgacgc gctaaacgac
     2161 catgaccaat ttcacgacgt tttggcccag attcacgacc tgtttcgccc acagagtaag
     2221 ctgggaagtt gtaatgcaac atgaagtttt c
//
LOCUS       DAOQMB010000386         2248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31243_length_2248_cov_5.010032, whole genome shotgun sequence.
ACCESSION   DAOQMB010000386 DAOQMB010000000
VERSION     DAOQMB010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31243_length_2248_cov_5.010032"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /gene="cas10"
                     /locus_tag="PLY05_10235"
     CDS             complement(<1..1383)
                     /gene="cas10"
                     /locus_tag="PLY05_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HNC03717.1"
                     /translation="MTTQENNTFWQTKLLARIHDPAEKAIVLMNTPEGHEGGTVADLK
                     KEFFNQAQLTELYPKVQKADHWAAAADRPTLPKADKWAQINFLNDAALIHPLSGEHFR
                     INGLSGTDIEAIKKASLAHSKSLIQRDKNGNIDYKKTNLALWRFQSEGKMLEGGWGAL
                     WEQLPADTRIPDHTIWTHLDLTSAFAGCFTAQESGDTPVLFSMQFGPVQDFIAQARST
                     SDLWAGSHLLSRLTWEGIKVLCQELGPDSVLIPQLRGVAMVDAWLYKEEGLAFDLFEQ
                     AGAEWTIKNDDSNPLFAAALPNKFTALVPKSKVKDLAEKIEQHVKSWVLKQGQDMLIK
                     LLLKADINPDDNLPCYKQLTEQLAGFPEVYWAAVPFDIISADSNGKATLKQDKLQEIQ
                     GSFIPVGKSFLETNVWGLLSKGLTDDNITFWQPNAGVLFPAIYELLERLTASVKQARH
                     FEQLQQKGYRC"
     gene            complement(1388..2215)
                     /locus_tag="PLY05_10240"
     CDS             complement(1388..2215)
                     /locus_tag="PLY05_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03718.1"
                     /translation="MVWYAENAKKALSDMQLAEALRFGSVEKGKNGKARIQIRFDEEA
                     KNPTGFVSGIRNDQNQSLQYLGFGPFVQPTNPSSKKALNIGAKVKFKVLIQGTNQQET
                     ETFTKELDKTMFLLQQFGTLGSRANNAWGSLHISGDIQPTSLTSYSLSLSQCLANDWK
                     KAITKDNKGLMIWHTNPFHNSDDAMKRLKTFRKEGHNSLAKQMGKRPLVNAPVKGNQN
                     RYPSQFVLKVIKEGGLYRGQVTLLAHKWHNDPRDLTAIITTIATYLDDHKDFERIKG"
BASE COUNT          600 a          460 c          449 g          739 t
ORIGIN      
        1 acagcgataa cctttctgtt gtaattgttc aaaatgacgt gcttgtttaa cactggcggt
       61 taagcgttcg agtaattcat aaatagcagg aaagagtacg ccagcatttg gttgccaaaa
      121 ggttatattg tcatcggtta agcctttaga taacaagccc catacgttag tctctaaaaa
      181 acttttgcca acagggataa agctgccttg aatctcttgt agcttatctt gcttgagtgt
      241 ggctttgccg ttgctatcgg cgctaataat atcaaacggc acagccgccc aatacacttc
      301 gggaaagcct gctaattgct cggtgagttg tttgtaacaa ggtaggttgt catcggggtt
      361 aatgtctgct tttagcaaaa gtttgattaa catatcttgg ccttgcttta aaacccaaga
      421 cttaacatgc tgttctattt tttcggctaa gtctttgact ttagatttgg gtactaaggc
      481 cgtaaattta ttgggcaggg cagcggcaaa taaggggtta ctgtcgtcgt ttttgattgt
      541 ccattctgcg cctgcttgct caaacaaatc aaaggctaat ccttcctctt tataaagcca
      601 cgcatcgacc atagctacac cgcgtaattg aggaatcagt acgctgtcag ggcctaattc
      661 ttggcatagt accttaatac cttcccatgt taagcgtgat aataaatgtg agcctgccca
      721 taagtcggag gtagaacgcg cttgagcaat aaaatcttgc actgggccaa actgcatcga
      781 aaataaaaca ggggtatcac cgctttcttg ggcagtaaaa caaccagcaa aagccgaagt
      841 taaatctaaa tgtgtccaaa tggtgtggtc tggaatgcga gtatcggcag gtaattgctc
      901 ccacaatgcg ccccagcccc cttcaagcat ttttccttcg gattggaaac gccataaggc
      961 taaattggtt ttcttatagt caatgttgcc atttttatcg cgctgaatga ggcttttact
     1021 gtgcgccaaa ctggcttttt taatggcttc tatatctgtg ccacttaagc catttatcct
     1081 aaaatgctca cccgataaag gatgtattaa agcggcatca ttcaaaaagt taatttgcgc
     1141 ccatttatca gctttgggta gtgtaggacg atcggctgcg gcggcccaat gatcggcttt
     1201 ttgtacttta gggtataact cagtcagttg agcttgatta aaaaactcct ttttaaggtc
     1261 agcgactgtg ccgccttcat ggccctctgg tgtgttcatc aacacaatgg ctttttcggc
     1321 agggtcgtga atacgagcga gaagtttggt ttgccagaat gtattatttt cttgagtagt
     1381 cattttttta tcctttaata cgttcaaaat ctttatggtc gtctaaatac gttgcaatag
     1441 tcgtaataat ggctgtcaag tctcgcggat cgttgtgcca tttatgagcc aataaagtaa
     1501 cttgtcctcg atatagacca ccttctttga ttactttcaa gacaaattga ctgggataac
     1561 gattttgatt tcctttaaca ggtgcgttaa ctaatggtct ttttcccatt tgctttgcta
     1621 atgagttatg cccttctttt cgaaaagttt tcaatctttt cattgcgtca tcactattat
     1681 ggaaaggatt tgtatgccaa atcatcaatc ctttattatc tttagtaatc gcttttttcc
     1741 agtcgtttgc taaacattga ctcaagctaa ggctgtatga tgttaggcta gttggttgaa
     1801 tatcacccga aatatgtaaa ctaccccacg cattattggc acggctaccc aaggtgccaa
     1861 attgctgtaa taaaaacatg gttttatcta attcttttgt aaaagtttca gtctcttgtt
     1921 gattggtacc ctgaatgaga actttaaatt taacttttgc accaatattt aatgcttttt
     1981 tactacttgg attggtaggt tgaacaaaag ggccaaaacc taagtattgc aatgattgat
     2041 tttggtcatt acgaatacca gaaacaaaac ctgttggatt cttagcttct tcatcaaaac
     2101 gaatttgtat tcgagcttta ccgtttttac ctttttctac actgccaaag cgtaaagctt
     2161 ctgctagttg catatctgat agtgcttttt tagcattttc ggcataccaa accactctcc
     2221 accactgtca aatcaacgct ttgatggc
//
LOCUS       DAOQMB010000387         2243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31368_length_2243_cov_16.970293, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000387 DAOQMB010000000
VERSION     DAOQMB010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31368_length_2243_cov_16.970293"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="PLY05_10245"
     CDS             complement(<1..227)
                     /locus_tag="PLY05_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03719.1"
                     /translation="MRLLIAEDTPSDRLILANLIKRLDHEVILAVDGKEAVELYNEHR
                     PDIVLLDVMMPYKTGTEVAREIRQIAGEELVP"
     gene            complement(254..559)
                     /locus_tag="PLY05_10250"
     CDS             complement(254..559)
                     /locus_tag="PLY05_10250"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNC03720.1"
                     /translation="MAILTTSSADGKELIIKIKGRFDFSTHQEFRNAYEQAPSEMSCY
                     VIDLKDATYLDSSALGMLLLLRDHAGGDSSQVRIINCNPDVKKILTISNFEQLFTIV"
     gene            965..2158
                     /locus_tag="PLY05_10255"
     CDS             965..2158
                     /locus_tag="PLY05_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNC03721.1"
                     /translation="MNQPYSAKKVALVLSGGGARAAYQVGVLKAISELSYSHCANLFP
                     IICGTSAGGLNAAGLACRADCLGEAVSQLEFVWSNFKTSQVYRTDWAGVLHCAARFLW
                     TMAFGRLHKDRPVSLLDNSPLYFLLERELLLSRLNDVISAEYLQALCITASGYTSGES
                     VSFFQGRADLEGWKRARRVGIPTLIRTEHLLASAAIPLLFPAIKLNREYFGDGALRQL
                     APMSPALHLGAEKILVIGVSNENNKERQKVDSYPSIAQVVANILNSSFIDSLESDIER
                     LTRINRTISHIPAEIREKNISLRHVDSLVIAPSSEIFDQIAMKHAMDLPRSIRTFVYG
                     SGATKRGGAGVLSYLLFEGAYCKELIDVGYADGMARATEIKQFLHLDQPSDHVCQLLD
                     FKNRA"
BASE COUNT          651 a          413 c          428 g          751 t
ORIGIN      
        1 ggaactagct cttcgcctgc aatttgacga atttcacgcg caacttcagt acctgtttta
       61 taaggcatca tcacatctaa caggacaata tctggccgat gctcgttgta tagctcaaca
      121 gcttcttttc catctactgc caaaatcact tcatgatcaa gacgtttaat taagttagcc
      181 aaaattaaac gatcacttgg cgtatcttca gcgattaaca gacgcataag ccacctccct
      241 gctttaagac tatttacaca attgtaaata attgctcaaa attagagatg gttaagattt
      301 ttttgacatc aggattacaa ttaatgattc tcacttgaga actgtccccc cctgcatgat
      361 ctcttaaaag caacaacatg cctaatgcag agctgtctaa ataagttgca tcttttaaat
      421 caatcacata acaggacatt tcgctgggtg cttgctcata agcattacga aattcttggt
      481 gggtgctaaa atcaaaacgc cctttaattt taataattaa ttctttgcca tcggctgaac
      541 ttgtggtaag tatcgccata aatatctccc tctacatgct gatgccatca ctattggtta
      601 tacagccaat aatattaaat tagtatattt gcttcttatt ctaaagcaaa ctttatacgc
      661 taagaaaggc tatcaggatg atttaattgt ttttttagaa catttgtttg gtttttatct
      721 gttttataca taccaaacac agtttgtatt tatacgatga ttttatctaa aacgctatag
      781 cacagttcac aaaaaactga ctgataagtt tctaattgat tcaatgcgaa aaaatcagca
      841 attactatac ttagactatg tgggcttacg tggctatctt tgaatagctt tcgcttgtaa
      901 agcgcggaac ttgctgagat atgcgtaaac tctgacgata aaaaccttgt ttaggagcaa
      961 caagatgaat cagccctatt ctgctaaaaa agttgcttta gttttgtctg gaggtggagc
     1021 cagagccgct tatcaagtgg gtgttttaaa ggcgatcagt gaattgtctt attctcattg
     1081 tgccaattta tttcctatta tttgtggcac atctgctgga ggcctcaatg ccgcaggttt
     1141 ggcttgccgt gctgattgtt taggagaggc agtttcacaa ttagaatttg tttggtcaaa
     1201 ctttaaaacc tcacaagtgt atcgcacaga ttgggctggc gttttgcatt gtgctgctcg
     1261 ttttttatgg acaatggctt ttggtcgttt gcataaagat cgacctgtgt ctttattgga
     1321 taattcacca ttatactttt tattagaacg cgaattatta ctttctcgtc ttaacgatgt
     1381 gattagtgcc gaatatttac aagctctttg tattacagca tcaggctata ccagtggtga
     1441 atcagtcagt ttttttcagg ggcgcgctga tttggaaggt tggaaacgcg ctagaagagt
     1501 tggtatccca acactaattc gcactgaaca tttattagca tcggctgcta ttcctctact
     1561 atttcctgcg ataaaactta atcgtgaata ttttggtgat ggagccttac gacaactcgc
     1621 gccgatgagt cctgccttac acttaggcgc agaaaaaatt ttagtgattg gtgtgagtaa
     1681 tgaaaataat aaagagcgtc aaaaagtcga tagctatcct agtattgctc aagttgttgc
     1741 taacatttta aatagctctt ttattgatag cttagaaagt gacattgaaa gactcactcg
     1801 aattaatcgt accattagtc atattcctgc tgagattaga gaaaaaaata tttcattacg
     1861 acatgttgat tctttagtga ttgccccttc tagtgaaatt tttgatcaaa tcgccatgaa
     1921 acatgctatg gatttgccac gtagtattcg aacatttgtt tatggcagtg gtgcaacaaa
     1981 acgtggcggc gcaggtgtgt taagttattt actatttgag ggagcttatt gcaaggagct
     2041 aattgatgtt ggttatgctg atggtatggc tagagcaact gagattaaac agtttttaca
     2101 tttagatcag cctagtgacc atgtttgcca gttactagac tttaaaaata gagcttaggg
     2161 attattagcg gctgttaaca ttttgcctgt gagcaaccac aatcaaaacg caaaacaatc
     2221 ctattatctt tatgcttttt tat
//
LOCUS       DAOQMB010000388         2226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31773_length_2226_cov_20.697835, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000388 DAOQMB010000000
VERSION     DAOQMB010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31773_length_2226_cov_20.697835"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PLY05_10260"
     CDS             complement(<1..346)
                     /locus_tag="PLY05_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03722.1"
                     /translation="MKIPPHIQVFGDVCFMGDCPTEDAELITFFNELAKLYPSLAKVA
                     IHPDNEGLVLGSGHLHHARQKAKGAIKKGAADIIICGNPTFVCEMKRQDHTKSRWQDG
                     QLEFLENAQRLGA"
     gene            complement(343..2205)
                     /locus_tag="PLY05_10265"
     CDS             complement(343..2205)
                     /locus_tag="PLY05_10265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC03723.1"
                     /translation="MNLRPYQQNAVDSAIQYIRKTKAPCLLELATGAGKSWIAAAIAH
                     WVTTNTHKKVLVLQPSKELTEQNYDKFLATGQKASIFSASAGSKCTRYDVVYGTPKTV
                     LNKIDRFGDKFACVIIDEAHMITPTIKEIIKRIKQRNNNLRVIGMTATPYRMYTGYIY
                     SQDETTGRALNDDLAINPYFASLIYSIKTRELISMGFLTEAHTEATTAHYDADKLELN
                     SRGQFNANEVSSVFENQERLTSLIVQDVMQKAQNRKGVMFFASTVRHAQEIMQSLPSD
                     NAMMLGGDINMDKRTREKLISDFKAQKYKYIVSVGTLTTGFDAPHVDCIAVLRATESA
                     SLFQQIIGRGLRLADGKNDCLVLDYAENIKRHDLFIDMFEPTIKTKKQGESECIDVSC
                     PFCGMTNSFALRKNPDKQKIDEQGYFLDLAGNRVLLGFDKKDKPLYMPAHYGRRCNGF
                     VKSPLNDGELWLCEYRWAYKECENCGHENDIAARYCENKQCKHELVDPNEKLNIQHAQ
                     ANRDSNKPIIERVLFFTVTKRVSKAGNNMLMVDYVTKNTNFRAFFVCDFDKAFVVKRW
                     KMLNESLFGQDVIFQSVHDFMDNYTNQMPITVTYKRNQNTKYIDVLNHNEELIA"
BASE COUNT          572 a          472 c          419 g          763 t
ORIGIN      
        1 acgcgcctaa tctttgcgcg ttctctaaaa actctaattg cccatcttgc caacggcttt
       61 ttgtgtggtc ttgtcttttc atctcacaaa caaaagtggg gttgccgcat attattatgt
      121 cagcagcccc tttttttatc gcgcctttgg ctttttgtcg cgcatgatgc aagtggccac
      181 tgccaagcac taagccctca ttgtcggggt gtattgcgac ttttgctagg cttggataaa
      241 gttttgcaag ctcattaaaa aatgtaatta gttcggcgtc ttctgttggg caatcaccca
      301 taaaacaaac atcgccaaaa acttggatat gcggtggtat tttcatgcaa ttaactcctc
      361 gttatggttc aaaacatcaa tatatttggt gttttggttg cgtttatatg tgacagtaat
      421 aggcatttga ttagtatagt tatccataaa atcatgcaca gattgaaaaa taacatcttg
      481 accaaatagt gactcgttta acattttcca tctttttaca acaaaagcct tatcaaaatc
      541 acagacaaaa aacgctctaa agtttgtgtt tttggtcaca tagtcaacca tcaacatatt
      601 gttgccagct ttgctaacgc gctttgtgac tgtaaaaaat agcacgcgct caataattgg
      661 tttgttagag tctctgtttg cttgtgcgtg ttgtatattt agtttttcgt tagggtcaac
      721 tagctcatgt ttgcattgtt tgttttcaca ataacgcgct gcaatatcgt tctcatgtcc
      781 gcaattttca cactctttat atgcccagcg atactcacac agccacaatt cgccatcatt
      841 taatggcgat tttacaaatc cgttacacct tctgccataa tgtgcaggca tataaagcgg
      901 tttatctttt ttatcaaaac ctagcaaaac tctattacct gccaaatcca aaaaataccc
      961 ttgttcgtca attttttgct tgtcgggatt ttttcgcaaa gcaaagctgt ttgtcatgcc
     1021 gcaaaatggg caagatacgt ctatacactc agactcgcct tgtttttttg ttttaatggt
     1081 tggctcaaac atatcaataa acaagtcatg ccgctttata ttctcagcgt aatccaatac
     1141 taaacaatca tttttcccat ctgcaaggcg caagcctcgc ccgataattt gttgaaataa
     1201 tgacgcgctt tcggtagctc taagcactgc tatacagtca acatggggcg cgtcaaaacc
     1261 agtggtcaat gtgccaacac tcacaatgta tttgtatttt tgcgccttaa aatcacttat
     1321 tagcttttcg cgtgtgcgct tatccatgtt tatatcgcca cccaacatca tggcgttatc
     1381 gcttggtagg ctttgcataa tctcttgagc atggcgcacg gttgatgcaa aaaacatcac
     1441 gccttttctg ttttgcgctt tttgcatgac atcttgcaca ataagcgatg ttaaacgctc
     1501 ttgattttca aacacgctag acacttcatt ggcattaaat tgtccacgac tgtttagctc
     1561 taatttgtca gcgtcataat gtgcggtggt tgcttcggtg tgcgcttctg ttaaaaagcc
     1621 catgcttatc aattcgcgtg ttttgatgct gtaaataagc gatgcaaagt aggggttgat
     1681 agctaagtca tcatttaatg cgcgtcctgt tgtctcgtct tgtgaataaa tataccccgt
     1741 gtacattctg tagggggtgg cagtcatacc aataacccta agattattgt tgcgctgttt
     1801 tattctttta ataatctcct tgatggttgg cgttatcatg tgcgcttcat caatgattac
     1861 acaagcgaat ttatcaccaa atctatcaat tttgttcagg acggttttag gtgtgccata
     1921 aacgacatca taacgagtac attttgagcc agcactggca ctaaaaattg atgctttttg
     1981 acctgtcgct aaaaatttat cgtaattttg ttcggtcaat tcttttgacg gttgcaaaac
     2041 taatactttt ttatgtgtgt tggttgttac ccagtgcgcg attgctgctg ctatccaaga
     2101 cttaccagcc cctgttgcta attctagcaa gcaaggtgct tttgttttgc gtatgtattg
     2161 tatggccgaa tcgactgcat tttgttgata tggtctaagg ttcatgtttg ctactcctta
     2221 gctcaa
//
LOCUS       DAOQMB010000389         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31956_length_2217_cov_10.846438, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000389 DAOQMB010000000
VERSION     DAOQMB010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31956_length_2217_cov_10.846438"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /gene="mreB"
                     /locus_tag="PLY05_10270"
     CDS             <1..743
                     /gene="mreB"
                     /locus_tag="PLY05_10270"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNC03724.1"
                     /translation="KKNMRVLVSVPAKATLLEHRAVREAVDGAGANDIRLIHQAMAAA
                     IGAGLPIDQACGSMVVNIGAGTTEIAVISLLGSVYTDSLRVGGDMMNERITTYIRRKY
                     GCLIGEATAEHIKQEIGMALVNPEQQQNTMEVRGRHLSAGVPTAIKVSAAEIAEVLQE
                     PLRNIINAIKNALEDMPAELSGDIAERGIVLTGGVAKLAQIDKLFSQEVGLPMILAEE
                     PESCVIRGMGQALAFLDNPAYDSLFVYS"
     gene            753..1661
                     /gene="mreC"
                     /locus_tag="PLY05_10275"
     CDS             753..1661
                     /gene="mreC"
                     /locus_tag="PLY05_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC03725.1"
                     /translation="MANPFDRQKRRFNQFPTHGYRLLMIGVIGGSVFWLNTRYPTVVE
                     PVHHYGQQLVEPFIRLLSYPQRVIDAIDQTLRTPQHLRQENKLLSRVIVQQSVELQQL
                     AELKAENDRLRALVNMQSRLDENLVNAEVVSSVVQLEQQVLMLNKGLREGLQVGYPVL
                     DAKGIMGRVIESSSRLAKVMLLTDSRHAIPVRVKRTQEHAILKGTGDHSRLRLEYAPD
                     SMDIKQGDILESSGMGQYYPAGYPVAVVIDVQKIGGTHFANVIAQALADINHSKQVIV
                     LFKQAPMIDDAPKIEALLPKNTSDVP"
     gene            1658..2134
                     /gene="mreD"
                     /locus_tag="PLY05_10280"
     CDS             1658..2134
                     /gene="mreD"
                     /locus_tag="PLY05_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNC03726.1"
                     /translation="MSRWTVQRVIMVSFLMGLLLSLFPLPLHIRQWWPEWLLLMAIFW
                     GIHQPQLFSVGTAFILGLVVDAITGSILAMHAIPYAMVVYLTRLWGQNFPLWTMIEQL
                     LFISFLLVVATLNHLFLLLFSPLMFSPLVVYIQILMSSLVAWFVINRSLVHWHRQF"
BASE COUNT          619 a          387 c          510 g          701 t
ORIGIN      
        1 ttaaaaagaa tatgcgtgtt ttggttagtg tacctgccaa agccacttta ttagaacatc
       61 gtgccgtacg cgaagctgta gatggtgcag gtgctaatga catccgttta attcatcaag
      121 cgatggctgc tgcgattggt gcaggcttac ccattgatca agcctgtggt tcgatggtgg
      181 tcaatattgg cgcaggtact accgaaattg ccgtgatctc cttattaggt tcggtttata
      241 ccgactcttt gcgtgtcggt ggtgatatga tgaatgaacg tattaccacc tatattcgtc
      301 gtaaatatgg ttgtttgatt ggcgaagcga cagcagaaca tattaagcaa gaaataggga
      361 tggctttagt taatcccgaa caacaacaaa atacgatgga agtcagaggg cgtcatttgt
      421 cggcaggtgt gccaacggca ataaaagtca gtgccgcaga aattgctgaa gtgttacaag
      481 agccgttgcg caatattatt aacgccatta aaaatgcttt agaagatatg cctgccgaat
      541 tatcgggtga tattgctgag cgtggtattg tgctaacagg tggtgtggct aaattagcgc
      601 aaattgataa attatttagc caagaagtag gtttgccaat gattttggca gaagaacccg
      661 aaagttgtgt cattcgtggt atggggcagg ctttagcctt tttagataat cctgcttacg
      721 acagtttatt tgtttattcc taaacaggcg atatggctaa tccttttgat cgacaaaaac
      781 gccgatttaa tcaatttcct acgcatggtt atcgactatt aatgataggc gtgattggtg
      841 gtagtgtatt ttggttaaat acccgttatc ccaccgttgt cgagccagtc catcattatg
      901 ggcagcaatt ggttgagcct tttattcgtt tattaagtta tccgcaacgt gtcattgatg
      961 cgatagatca aaccctacgt actcctcaac atttacgcca agaaaataaa ttattatcac
     1021 gcgtgattgt ccagcaatcg gttgagttac aacagcttgc cgagctaaaa gcagaaaatg
     1081 accgtctgcg cgctttagtt aatatgcagt cgcgcttaga tgaaaatttg gttaatgcgg
     1141 aggttgtgtc ttccgttgtg cagttagagc aacaagtatt aatgctcaat aaaggtttaa
     1201 gagaaggttt acaggttggt tatcccgttt tagatgcaaa aggcattatg ggacgtgtga
     1261 ttgagtccag tagtcgttta gcgaaagtga tgttgttaac cgatagtcgc catgcaattc
     1321 ctgttcgagt aaaacgtact caggaacacg cgattttaaa aggtacagga gatcatagtc
     1381 gtttgcgttt agaatatgca ccagactcga tggatattaa acaaggagat attttagaaa
     1441 gctcaggcat ggggcagtat tatcctgctg gttatcctgt tgctgtagtg atagatgtac
     1501 aaaaaattgg tggaactcac tttgccaatg tcattgcaca agcacttgca gatatcaatc
     1561 acagcaaaca agtgattgta ttgtttaaac aagcaccaat gattgatgat gccccaaaaa
     1621 tagaagcttt attgccgaaa aatacgagtg atgtgccatg agtcgttgga cagtacaacg
     1681 agtcataatg gtaagttttc ttatgggctt gttgttatcg ctgttcccct tgccgttgca
     1741 tattcgacaa tggtggccgg aatggttgtt gttaatggct attttttggg gaattcatca
     1801 gccacaactg tttagtgtcg gaacagcttt tatattaggt ttagtggttg atgcaatcac
     1861 aggtagcata ttagcgatgc acgccattcc ctatgcgatg gttgtctatt taacaaggct
     1921 ttggggacaa aactttccat tatggacgat gatagaacaa ttattattca ttagtttctt
     1981 gttagtagta gctacattaa atcatctgtt tttattgttg ttttcgccac tcatgtttag
     2041 tccattagtg gtgtatatac aaatcttgat gtcaagttta gtggcttggt ttgttattaa
     2101 tcgtagtttg gtgcattggc acagacaatt ttaagagtca ttatatgctt tatttagcat
     2161 ccacatccgt tccataaaac ttatcaaaac ctacttattg tagaacaaat aggcttt
//
LOCUS       DAOQMB010000390         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_31971_length_2216_cov_7.085146, whole genome shotgun sequence.
ACCESSION   DAOQMB010000390 DAOQMB010000000
VERSION     DAOQMB010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31971_length_2216_cov_7.085146"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="PLY05_10285"
     CDS             <1..2057
                     /locus_tag="PLY05_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC03727.1"
                     /translation="GGQGNHLGGGYGDATLKALGVKYRSNALAQEKTGEFWVQGKMFG
                     TGAHGDFHHCEVAVFLGKNPWQSHGFAQTRVLLKEIQKDPKRAMIVIDPRVSETAAMA
                     EYHLQVKPATDAWCLAALIAIIVQNNWHKADWLAQHTTNFAKIAPLFAKINIAEYAQI
                     CGVELSLLQAAAQRIAQAKSVSIFEDLGTQMNQHSTLNSYLQRILAVVTGNYGKEGTA
                     NAFVPFFSLAKASKGETGGSSKKTPPSPSNKGRLSPVTQSKIIIGLIPCNVIPDEILT
                     DHPKRFRAMIVESGNPVHSLADSQRMREAMRALEFSLVIDVAMTETARQASYVLPASS
                     QFEKAEATFFNIEFPKNAFHVRQPLFEPLAGTLPEAEIHARLLEAMGELSEQDYLPLR
                     MALKLDQVKFGQRKLGELQLLKEPLARKAFMAAFVSQAALSKKFVPYAPVILYRTLGQ
                     TLPKGMETAALVWALSLFHVMDNPKTAARVGFAGLAPLAGDRLFQAIIDNPSGVVYAD
                     RTYAESWQAIKRPENRIELYIAELVGEIEKLATDKPEAVNPDYPFVLSAGERRNDTAN
                     TAIRDASWHKRGRFGTLRINAQDAENLGCVDGDLVQLSTARGSVQVPLEVSKMMQRGH
                     VSLPNGQGLHYTNSNGEVIQQGIAPNELTDNQRRDFFAGTPWHKSVPARIEKVNVIQI
                     QE"
BASE COUNT          646 a          457 c          506 g          607 t
ORIGIN      
        1 gcggtggcca aggcaatcat ttaggcggtg gttatggtga tgccacactc aaagcgttag
       61 gcgttaaata ccgttctaat gccttagccc aagaaaaaac aggcgaattt tgggtacaag
      121 gcaaaatgtt tggcacaggt gcacacggtg attttcatca ttgtgaagtg gcggtatttt
      181 taggaaaaaa tccttggcaa tcacatggtt ttgcacaaac cagagtgttg ttaaaagaaa
      241 ttcaaaaaga ccctaaacgc gcaatgattg tcattgaccc acgtgtcagt gaaacggcag
      301 caatggccga ataccactta caagtcaaac ctgccactga cgcatggtgt ttggccgcgt
      361 taatcgccat tattgtgcaa aataattggc acaaggccga ttggttggca caacacacca
      421 ccaattttgc caaaattgcc ccgttatttg ccaagataaa tattgccgaa tatgcccaaa
      481 tttgtggggt agagctaagt ttattacaag cggctgctca acggattgct caagcaaaaa
      541 gtgtgtcgat ttttgaagac ctcggcacac aaatgaatca acattcaacc ttaaatagtt
      601 atttgcaacg tattttagca gtagtgacag gcaattatgg caaagaaggc acagccaatg
      661 cttttgtgcc gtttttctcc ttagccaaag ccagtaaagg cgaaacaggt ggcagcagca
      721 aaaaaactcc accatcaccc agtaataaag gccgtttgag tcctgttact caatctaaaa
      781 ttattattgg tctcattccg tgtaatgtca ttcctgatga aattttaacc gatcatccta
      841 aacgttttag agcgatgatt gttgaaagtg gtaatcctgt gcattcttta gccgatagtc
      901 aaagaatgcg tgaagccatg cgcgctctgg aatttagttt ggtgattgat gttgcgatga
      961 ccgaaacggc acgtcaagcg agttatgtgt tacctgcatc aagtcagttt gaaaaagccg
     1021 aagccacttt ttttaatatt gaatttccta aaaatgcctt tcatgtccgt caacctttgt
     1081 ttgagccatt agcaggcaca ttgcccgaag ccgaaattca tgcacgttta ttagaagcga
     1141 tgggcgaatt atccgaacaa gattatttac cgttacgcat ggcacttaaa ctcgaccaag
     1201 ttaaattcgg tcaacgcaaa ttaggtgagt tacaactatt aaaagagcca ttagcgcgta
     1261 aagcctttat ggcggcattt gtgagccaag cagcactatc gaaaaagttt gtaccttatg
     1321 cacctgtgat tttataccgt actttaggcc aaaccttgcc taaaggcatg gaaacagcgg
     1381 ctttagtgtg ggcattgtcg ttatttcatg tcatggataa tcccaaaacc gccgcacgag
     1441 tgggttttgc agggctcgca ccattagctg gtgaccgatt gtttcaagca attatagata
     1501 atccgtcggg cgtggtgtat gccgaccgta cttatgccga aagttggcaa gcgattaaac
     1561 gtcccgaaaa ccgtattgag ttatatattg ctgagttagt tggtgaaata gaaaaactcg
     1621 ccactgacaa acccgaagca gttaatcctg attatccttt tgtgttgtcc gcaggtgagc
     1681 ggcgtaatga taccgcgaat accgccattc gtgatgccag ttggcataaa cgtggtcgtt
     1741 ttggtacttt gcgtattaat gcccaagatg ccgaaaattt aggctgtgtt gatggtgact
     1801 tagtgcaatt aagtactgcc agaggctctg tgcaagtgcc actagaagta tcaaaaatga
     1861 tgcaacgagg tcatgtgtca ttacctaatg gccaaggttt gcattatacc aatagcaatg
     1921 gtgaagtaat tcagcagggt attgctccaa acgagttaac cgacaatcaa cgccgtgact
     1981 tttttgcggg cacaccgtgg cataaatctg tgcctgctcg tatagaaaaa gtcaatgtga
     2041 tacagattca agagtaaaac cactgggggg agtcatgtac ataatcaatt tttggagaaa
     2101 aatatgaccg cagctcaggt ttttaatggc gcagtcaatg tgccaaccaa tgcaagcaat
     2161 ttagcaaccg cttgtgcctt gtgttcacat aattgtggct tacgtgtcga tgtggc
//
LOCUS       DAOQMB010000391         2207 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_32245_length_2207_cov_4.180762, whole genome shotgun sequence.
ACCESSION   DAOQMB010000391 DAOQMB010000000
VERSION     DAOQMB010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32245_length_2207_cov_4.180762"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLY05_10290"
     CDS             <1..725
                     /locus_tag="PLY05_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004670550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC03728.1"
                     /translation="GTDPDSDLAVIKIDLKDLPTIPFKSSANEVGDVVLAIGNPFGVG
                     QTVTQGIISALGRNGLGINTFEDFIQTDAAINPGNSGGALVDVAGNLVGINTAIYSRS
                     GGSMGIGFAIPSQLAQSVMMEIISHGQVVRGWLGVEIKQDNAGFNEDGSLNNKVSEGV
                     EIGGIVRGGPADEGGIRPNDMILSINNQKVSSANQLMNTIAKIKPDSKINVVVKRGNQ
                     EVELGVTVGKRPKMEQSQIDEE"
     gene            complement(765..>2207)
                     /locus_tag="PLY05_10295"
     CDS             complement(765..>2207)
                     /locus_tag="PLY05_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNC03729.1"
                     /translation="QDVGRGTHQLVAARYTFRRLFLVGLTALLADAVGFAVLMVIDIP
                     VIRELALTASIGVAVLIFTNLILLPVLLSYTGVSPTAAQASLTIEEQEKTGKGLGRVW
                     GLLNKFTERKYATIAVSTAGVLTIVGLALSTQVKVGDLDAGAPELRPNSQYNLDNAYI
                     TQNYALSSDVFAVIVKTNPDGCMKYQTLVAADRLAWALEQQQGVQGTSSLVNAVRQIT
                     AGSYEGNPKWFTINRNQDVINYGAQQASTSWPDLFNFECSVMPVIAYLHDHKADTLSR
                     VAATAEEFAKQNSTPDQQFLLAAGSAGIDAAINSVVKEANRQMLLYVYAAVIALCWLA
                     FLSWRAVVVAVIPLMMTSILCEALMVILGIGIKVATLPVIALGVGIGVDYALYLLSLQ
                     LANQRRGEPLTQAYQSAVRFTGKVVALIGVTLAGGVVTWIFSPIKFQADMGILLTFMF
                     ILNMIGALVLIPALSHFLLPTEKVFKEETV"
BASE COUNT          694 a          463 c          434 g          616 t
ORIGIN      
        1 ttggcacaga ccccgacagc gatttggcgg tgattaaaat tgatttaaaa gacttaccca
       61 ctattccttt taaaagctct gccaatgaag ttggtgatgt ggtgttagcg attggtaatc
      121 cttttggtgt tggccaaacg gtaactcaag ggattatttc ggctttaggg cgtaatggtt
      181 taggcattaa tacttttgaa gattttattc aaaccgatgc agcgattaat cctggtaatt
      241 cgggtggggc attagtcgat gtggcaggta atttagttgg gattaataca gcaatctact
      301 ctcgctctgg tggctcaatg ggcattggtt ttgccattcc ctcacaatta gcacaatcgg
      361 tgatgatgga aattattagt catggtcaag tggtgcgtgg ctggttaggt gttgaaataa
      421 aacaagataa tgctggtttt aatgaagatg gctctctaaa taataaagtt agcgaaggcg
      481 tagaaattgg aggcattgtc cgtggtggtc ctgcggatga aggcggcata cggcctaatg
      541 atatgatttt aagtatcaac aaccaaaaag taagttctgc caatcaactc atgaatacta
      601 ttgccaaaat taaacctgat agcaaaatta atgtggtagt caaacgcggt aaccaagagg
      661 ttgaattagg cgtgaccgtt ggcaaacgcc ctaaaatgga acaaagtcaa attgacgagg
      721 aatgataaaa tgttcccatt gaaaacaatg ggaacagcta ttatctaaac cgtttcctct
      781 ttaaacactt tttcggtagg taacaaaaag tgtgataaag cgggaattaa cactaatgca
      841 ccaatcatgt ttaaaataaa cataaaggtt aacaaaatgc ccatatccgc ttggaattta
      901 atgggtgaga aaatccatgt gacaacacca cctgctaagg ttaccccaat tagcgcaacc
      961 actttacccg taaaacgtac tgccgattga taagcctgag ttaatggttc accacgacgt
     1021 tgattagcta attgtaaact taacagatat aaggcgtaat ccacaccaat acccacccct
     1081 aacgcaatta caggtaaagt cgcgacttta ataccaatac ctaaaataac cattaatgcc
     1141 tcacacaata tcgaagtcat cattaatgga atcaccgcca ccaccactgc gcgccaactt
     1201 aaaaaagcca accaacataa agcaatcact gctgcataaa catacaacaa catttgacgg
     1261 ttagcctctt tcaccacact gttaatggca gcatctatcc ctgcactacc tgcggctaat
     1321 aaaaattgct gatcgggtgt gctattttgt ttggcaaact cttcggcagt ggcggccact
     1381 cggcttaaag tgtcggcttt atggtcgtgt aaataggcaa taacaggcat caccgaacat
     1441 tcaaaattaa ataaatcggg ccaactggta ctagcttgtt gtgcgccata gttaatcaca
     1501 tcttgattac gattaatggt aaaccacttt ggatttcctt cataactgcc tgcggtaatt
     1561 tgacgcactg cattcactaa agaacttgta ccttgtacac cttgttgctg ctctaaagcc
     1621 catgctaaac gatcagccgc cactagcgtt tgatatttca tacaaccatc aggattggtt
     1681 ttgacaatca ctgcaaacac atcactggat aaagcataat tttgggtaat atacgcatta
     1741 tctaaattat attgtgagtt tggtcggagt tctggtgcgc ctgcatcaag gtcccctact
     1801 ttgacttggg tactcaatgc caaaccaaca attgttaata ctccagcagt actgacagca
     1861 atcgttgcat atttacgttc ggtaaattta tttaatagcc cccatacacg acctaaacct
     1921 ttacctgttt tttcctgttc ttcaattgtt aaagaagctt gtgcggcggt tggacttaca
     1981 cctgtatagg acaataaaac aggtaataaa atcaaattgg taaaaattaa gactgctaca
     2041 ccaatactag ccgtgagtgc taattctcga ataacaggta tatctatcac catcaatacg
     2101 gcaaaaccga ctgcatcggc taataatgca gttaatccca ctaaaaataa acgtctaaat
     2161 gtataacgtg cggccactaa ttgatgtgta ccacgtccga catcctg
//
LOCUS       DAOQMB010000392         2202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_32380_length_2202_cov_4.100605, whole genome shotgun sequence.
ACCESSION   DAOQMB010000392 DAOQMB010000000
VERSION     DAOQMB010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32380_length_2202_cov_4.100605"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLY05_10300"
     CDS             <1..599
                     /locus_tag="PLY05_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002116854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNC03730.1"
                     /translation="MLAIVWFDKIRRNPEDHASPEVKERRVGFEEGVRMATNDTIGHI
                     GALIMLMALSVSIGGVIERSGMMDAVPESFGSVYLAVSILLVLLVFVGMVMDPFGAVI
                     LVSATVAPIAYKNGIDPVHFWMIVLTAFELGYLSPPVALNQLLTRQVVGEEVMDEADA
                     EVRHLSFYWRYERWILPCMVMAPALLLVAYVPLFFYAK"
     gene            658..1230
                     /gene="rrtA"
                     /locus_tag="PLY05_10305"
     CDS             658..1230
                     /gene="rrtA"
                     /locus_tag="PLY05_10305"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HNC03731.1"
                     /translation="MSKINISLIITLWLVLCSLQMIGINHFYFERDLLGQGQYWRLLT
                     AHFVHLNVTHLVFNLAGLSLVLIILDRTWSLAAWLCLIVFSAVAQSLAFYWFLPHVKA
                     YVGFSGVIHSLYVAGAVILLQKRQDRYLAIVLLILVTLKLLTENIGQGISVTEKMIGG
                     HVLVEAHLYGAIIGLIYALVALSFRRIKDN"
     gene            1300..1971
                     /locus_tag="PLY05_10310"
     CDS             1300..1971
                     /locus_tag="PLY05_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNC03732.1"
                     /translation="MFRWFSLLVVWCCLFTNSVLAEESSSTDKLILDKNNIKIWSYKV
                     PQSPLYGFKAVTTVKSTLSGLVSLITDTQNANHWLYRTSKIDVLARDDGQQFFTIRVI
                     TDVPWPLTDREALVDVRITQDKTGRVRIDSNESVSANKYPVADCCLRMPMVKGYWLFK
                     PVGNGMVEVTMSGHADPGGMIPKGLVNFLIQEHPYNTLQGLRRVIGDVGYQKAQYPHI
                     VESVQ"
     gene            1982..>2202
                     /locus_tag="PLY05_10315"
     CDS             1982..>2202
                     /locus_tag="PLY05_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03733.1"
                     /translation="MDELVVIAKPPKKKTTSLKQWVWRIIGGLVVILVTYHLWLLFSI
                     WWWIDHNPQSTSFMETQREVLQDKNPDAE"
BASE COUNT          592 a          341 c          498 g          771 t
ORIGIN      
        1 ttatgttggc aattgtctgg tttgataaaa ttcgtcgcaa tccagaagat catgcttcac
       61 cagaagtcaa agagcgtcgt gtaggctttg aagaaggcgt acgtatggca accaatgaca
      121 ctattggtca tattggtgca ttgattatgc tcatggcttt atcggtcagt atcggtggtg
      181 tgattgaacg ttcaggtatg atggatgcag tgccagaaag ctttggtagt gtttatcttg
      241 ctgtttctat tttattagtg ttgttagtct ttgttggtat ggtgatggat ccattcggtg
      301 cggttatttt ggtgtcagca accgttgcac cgattgccta taagaatggt attgatcctg
      361 tccatttctg gatgattgta ttaactgctt ttgaattagg ttatttgtct ccacctgttg
      421 ccttgaacca actcttgact cgtcaagtag tcggtgaaga ggtgatggat gaggctgatg
      481 ccgaggtacg tcacttatcc ttctactggc gttatgagcg ttggatttta ccttgtatgg
      541 taatggctcc agccttattg ttagtagcct acgtaccgtt gttcttctac gctaaataaa
      601 tgtgtattta gcaaaagggc cagcattcgc tggccttttt tttagagcta aatttaaatg
      661 tccaaaatca atatcagctt aattatcacc ttgtggttag ttttatgtag tctacaaatg
      721 atcggaataa atcattttta ttttgagcga gacttattag gacagggaca atattggcgt
      781 ttattaacag cacattttgt tcatttaaat gttacgcatc tcgtttttaa tttggctggt
      841 ttaagcttag tgttgattat tttggatcgt acttggtctt tggcggcatg gttatgctta
      901 atcgtttttt cggcagttgc acaaagtctg gcattttatt ggtttttgcc acatgtcaaa
      961 gcttatgttg gtttttctgg cgtgattcat agtttatacg ttgctggtgc ggtgatcttg
     1021 ctacaaaaac gccaagatag atatttagca atcgttttat taattctagt cacattaaaa
     1081 ctactgacag aaaatattgg acaaggtata tctgtcactg agaaaatgat tggtggtcat
     1141 gttttggtcg aagctcattt atatggtgca ataataggct taatctatgc cttagttgca
     1201 ttgagtttta gaagaatcaa agataactaa taatagtgca tattctacgt tttaattttc
     1261 acagtagtgc ttaagcctaa tataagagaa gccttattca tgtttagatg gtttagtctt
     1321 ttagttgttt ggtgttgttt atttactaat tctgttttag ctgaagagtc gagctcaaca
     1381 gataagctaa ttttagataa aaataatatt aaaatttgga gctataaagt gccacaaagt
     1441 cctttatatg gctttaaagc agtcacaaca gtaaaaagca ctttatcagg attggtgagt
     1501 ttaattactg atactcaaaa tgcaaatcat tggttatatc gtacgagtaa aattgatgtc
     1561 ttggcgcgtg atgatggcca gcagtttttt actattcgtg tgattaccga tgtgccgtgg
     1621 cctctcacag accgtgaagc gctagtggat gtgcgcatta ctcaagacaa aacggggcga
     1681 gtgcggattg atagcaatga gtctgtgagt gcgaacaaat atcctgtcgc tgattgttgt
     1741 ttaagaatgc cgatggttaa aggctattgg ttatttaaac ctgtaggtaa tggcatggtt
     1801 gaggtaacca tgtcggggca tgccgaccct ggtggaatga ttccaaaagg cttggtaaac
     1861 tttttaattc aagagcatcc atataatacc ttacaaggtt tacgtcgtgt tattggtgat
     1921 gtaggctatc aaaaagcaca atatcctcat attgttgaaa gtgttcaatg agtgaataac
     1981 tgtggatgag ttagttgtaa ttgctaaacc acctaagaaa aaaacaacaa gcctaaaaca
     2041 atgggtttgg cgtattattg gtggtttggt ggttattttg gtgacttatc atttatggtt
     2101 attgttcagt atttggtggt ggattgatca taatccacaa tctacctcgt ttatggaaac
     2161 acagcgtgag gtcttacaag ataaaaaccc tgacgctgag tt
//
LOCUS       DAOQMB010000393         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_32554_length_2194_cov_6.558205, whole genome shotgun sequence.
ACCESSION   DAOQMB010000393 DAOQMB010000000
VERSION     DAOQMB010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32554_length_2194_cov_6.558205"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="PLY05_10320"
     CDS             complement(<1..1157)
                     /locus_tag="PLY05_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNC03734.1"
                     /translation="MKKILLLGALLPNVMWAQVEMPFSALLPPENEVRQSLEQHPMVQ
                     ANQATMDYERANAQRLIQGEDPWQARFALQQRRNNQANLNSVEQTMGLEKRLRLPSKV
                     AMDKQLAQTALSIAQLSRGDAWHEAAKILLADWFAVSKAQANLQRIDEQLALLDKQLA
                     VINKRISAGDAPRLERLLVEQERAKLALQRQQQHAELAQQQLNLQRYYRGQPLSYIDL
                     SQIPSQLNLGDEQTWIKQVLAANHELELAEEHSKRQDLHTQRVALENKPDPTLGITYS
                     REQAGQENIIGLSVSVPLFNPAHKSLVNMAQAQASQAHYQQQQTAQRLTQETKQRLLS
                     LSAAQQQLQQAQDLVTQQQANSQLFAKAYQLGEVSINDWLLSMRQYVEASQ"
     gene            complement(1159..1473)
                     /locus_tag="PLY05_10325"
     CDS             complement(1159..1473)
                     /locus_tag="PLY05_10325"
                     /inference="COORDINATES: protein motif:HMM:NF023014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3240 family protein"
                     /protein_id="HNC03735.1"
                     /translation="MSQQVLLQLNISARIRAEFLDHLRLVPHLVSGCTIVDAAGFGAQ
                     QHYGSALEKVLGQTQRQLVMMVLAQDNVSAVLAHIRQQFISPDIQYWLTPVLASGRLS
                     EV"
     gene            complement(1476..>2194)
                     /locus_tag="PLY05_10330"
     CDS             complement(1476..>2194)
                     /locus_tag="PLY05_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03736.1"
                     /translation="QSIALSSVATLVADEGVVKIERENASRMSVIRANVKGRDLVGFV
                     DEAKAKVQQQVKLPVGYRLAWGGQFENQQRAAARLMTVIPAALAMIFFLLFATLGSVR
                     QAALVFVNIPFALIGGVVALAITGQYLSVPASVGFIALMGIAVLNGLVMIGYFNQLLA
                     RGLDVATVVKQGAMRRLRPVMMTASIAAFGLVPLLFATGPGSEIQQPLAVVVIGGLVS
                     CTLLTLVLLPNLFRRFGVER"
BASE COUNT          603 a          527 c          447 g          617 t
ORIGIN      
        1 tgctgactgg cctcgacata ctgacgcata cttaacagcc aatcattaat actcacttcg
       61 cccaactgat aggctttagc aaataactga ctattggctt gttgctgtgt gactaagtct
      121 tgtgcctgtt gtagttgctg ttgtgctgcc gacaaactca ataaacgttg tttggtttct
      181 tgtgtcaaac gttgtgccgt ttgttgttgt tgataatggg cttgactggc ttgagcttga
      241 gccatattta ctaaggattt atgcgcagga ttaaacaacg gcacactgac acttaagccg
      301 ataatatttt cttggccagc ttgctcacgg ctataagtaa tccctaatgt ggggtcaggt
      361 ttgttttcta aagccacacg ctgggtatgt aggtcttgac gtttactatg ctcttcggcc
      421 agttctaatt cgtggtttgc ggctaagact tgcttaatcc atgtttgctc atcgcccaaa
      481 tttaattgac tgggtatttg acttaaatct atatagctta acggttggcc acgataataa
      541 cgctgtaaat ttaactgttg ttgtgctaat tcggcgtgtt gctgttgacg ttgcaaagcc
      601 aactttgccc gctcttgctc aaccaatagt cgctctaaac gaggtgcatc acctgcacta
      661 atgcgtttat taatcactgc taactgttta tctaataagg ctaattgctc atctatacgt
      721 tgtaaattcg cttgcgcttt gctaaccgca aaccaatcag ccaataatat ttttgctgct
      781 tcgtgccacg catcaccacg actgagctga gcgatgctta gtgccgtttg tgccagttgt
      841 ttatccatcg ctactttgct gggtaaacgt aaacgttttt ctaaacccat agtttgttct
      901 acactattta aattagcttg attattacgg cgttgttgta aggcaaatct tgcttgccac
      961 gggtcttcac cctgaattaa acgctgggca ttagctcgtt catagtccat ggttgcttga
     1021 ttggcttgta ccatcggatg ttgctctaga ctttgacgca cctcattttc tggcggtaat
     1081 aaagcactaa atggcatctc cacttgcgcc cacatcacat tcggcaacaa agcacccaac
     1141 aaaagtattt ttttcatctt agacctcact caaacgacca ctagccaaca caggtgttaa
     1201 ccaatattga atatcaggcg agataaattg ttgacgaata tgggctaata ccgcagacac
     1261 attgtcttgg gctaatacca tcatcaccaa ttgccgttgg gtttgaccta agactttttc
     1321 taaagccgaa ccatagtgtt gttgtgcgcc aaaacctgcg gcatcgacaa tggtgcaacc
     1381 cgagactaag tgtggcacta agcgtaaatg atctaaaaat tcggcacgaa tccgcgcgct
     1441 aatgttgagt tgtaataaca cttgctgact catgattaac gctccacacc aaaacgacga
     1501 aataaattag gcaacaacac caaggttaat aaggtacaac tgactaatcc gccaatcacc
     1561 accaccgcta aaggttgttg aatttccgaa cccggccctg tggcaaataa caacggtact
     1621 aaaccaaaag cagcgatgct agcggtcatc atcacaggcc gcaaacggcg catagctcct
     1681 tgtttaacca cggtagcaac atctaaacca cgcgccaata attgattaaa ataaccaatc
     1741 atcaccaagc cattgagtac ggcaatgccc attaaggcaa taaaacccac tgaggcaggc
     1801 acagacaaat attgtcctgt gatcgctaaa gccactacgc caccaattaa agcaaaagga
     1861 atattgacga acactaaggc ggcttggcgc actgagccta aggtggcaaa taataagaaa
     1921 aatatcatcg ctaaggcggc aggaatcacc gtcattaacc gtgcggcggc acgttgttga
     1981 ttttcaaatt gtccacccca tgccaaacga taaccgacag gtaatttaac ctgttgttgg
     2041 actttggctt tggcttcgtc gacaaaaccc accaagtcac ggcctttgac attggcgcga
     2101 atcactgaca tccgtgaggc attttcacgc tcaattttga ccacgccttc atctgccact
     2161 aatgtggcca ctgaggacaa tgcaatactt tggc
//
LOCUS       DAOQMB010000394         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_32750_length_2187_cov_10.636961, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000394 DAOQMB010000000
VERSION     DAOQMB010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32750_length_2187_cov_10.636961"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="PLY05_10335"
     CDS             <1..209
                     /locus_tag="PLY05_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03737.1"
                     /translation="LKIWSAQAQEDVYWRQVLPETEREQLPSEFIEQALELYQPIFNH
                     SKALNWRTLAQDMDAIECSRIVNN"
     gene            263..529
                     /locus_tag="PLY05_10340"
     CDS             263..529
                     /locus_tag="PLY05_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004678281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNC03738.1"
                     /translation="MQNTAAVERIHLRASLEAKQTIEQAAALSGVSVSAFVLEHAYQA
                     ARKAVLEQQQISLGREDWAALEKALENPAPPTKTMLDFMRGENI"
     gene            529..1014
                     /locus_tag="PLY05_10345"
     CDS             529..1014
                     /locus_tag="PLY05_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC03739.1"
                     /translation="MFDIDVLSRQYQRDRFDCGQVDLNTFIKQYALQQQGRFLSQTYV
                     AYNPTKQVIGFYSLANGSTLRDDLPITEQKRLPRYPIPCVIIGRFAVDINYQGKGLGQ
                     ALLRHAFKKIIDISTLTGTAYILVHAKDDKAASFYQKLGFQSFPQQALTLFLPVASLP
                     R"
     gene            1035..>2187
                     /locus_tag="PLY05_10350"
     CDS             1035..>2187
                     /locus_tag="PLY05_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNC03740.1"
                     /translation="MTKQIPALNCPLYGVQLIEASAGTGKTWTLSALFARLIVQKKLE
                     VKHILAITFTKAAAAELRDRIRKRLLEVKYTLEQEQPNPEEVVGDYLYGWLQKNGGKQ
                     QGIDRLQNAIVNFDEATITTINGFCQGELVEQALASGMPFETTLISDTAPILHDIIQD
                     YCRQQWRQAEPLLVNYFLSKEEFSLSSLYAFVSGILHQGQARVLKPSFLFNGQLYRQT
                     EQWFNKLQQLWQQQGREISQLFYQHKHTLNGNKYREDSVTKWLNGLDLAMSSGLMELL
                     SNDDAQTAIHKLSYSTLQESLKKNSPDFPQHRFFDDCEHLAELLAKTDKQLQLQGIAF
                     KCDLLIHSGKALLERLHAKQAQSFDMQIKGLAKALTHADTGQVLAQALRQ"
BASE COUNT          687 a          423 c          439 g          638 t
ORIGIN      
        1 ctctaaaaat atggagcgca caagcccaag aagatgtgta ttggcgacaa gtgttacctg
       61 aaaccgaacg tgaacagttg ccttcggagt ttattgagca agccctagag ttatatcagc
      121 cgatttttaa tcatagcaag gcattaaatt ggcgaacctt ggcgcaggat atggacgcta
      181 tagaatgctc tcgaattgtt aataattaat tataagtcta taatgtacgc tatatcggtg
      241 tacatttttg ggggagctta tcatgcaaaa tacagccgct gtagaacgta tacatttacg
      301 agcctcactt gaagctaaac aaacaataga gcaagccgcc gctttatctg gcgtgagcgt
      361 gagtgccttt gtcttagaac atgcctacca agctgctcgt aaagcggttt tagaacaaca
      421 acaaattagc ttaggacgtg aagattgggc agcactagag aaagctttag aaaaccctgc
      481 accacccacc aaaacaatgc ttgattttat gcgtggagaa aatatctaat gtttgatatt
      541 gatgtactca gccgacaata tcaacgtgat cgttttgatt gtggacaagt tgaccttaat
      601 acatttatta agcaatatgc tttacagcaa caaggtcgtt ttttaagcca aacctatgtc
      661 gcttataatc ctacaaagca agtaattggc ttttatagtt tagctaacgg tagtacattg
      721 cgtgatgatt taccaataac agaacaaaaa cgattacctc gttatcccat tccttgtgtg
      781 attattggtc gttttgcggt ggatataaat tatcaaggta aagggctagg gcaagcttta
      841 ttacgccatg cttttaagaa aattatcgat atatccacac tgacaggaac agcctatatt
      901 ttggtacatg ctaaagatga taaagcagca tctttttatc aaaagcttgg ttttcaatca
      961 ttcccacaac aagcattaac cttatttttg cctgttgcat cactgcctcg ctagtctgag
     1021 ttggcgcaat taatatgact aaacaaattc ctgccttaaa ttgtccttta tatggcgtgc
     1081 agttaattga agcttccgca ggaacaggta aaacgtggac attgtcggca ttatttgcgc
     1141 gtttaattgt gcaaaaaaaa ctcgaagtca aacatatttt agctatcact tttaccaaag
     1201 cggcggcggc agagttgcgc gaccgtatta gaaaacgttt attagaagtt aaatataccc
     1261 tagagcaaga gcagccaaat cctgaagagg tggttggtga ttatttgtat ggctggttgc
     1321 aaaaaaatgg tggtaaacaa caaggtatcg accgcttaca aaacgccatt gttaactttg
     1381 atgaagctac catcaccacc attaatggtt tttgccaagg tgaattggtt gagcaagctt
     1441 tagcgagtgg tatgcccttt gaaaccacgt taattagtga taccgcaccc attttacatg
     1501 acattattca agattattgt cggcaacaat ggcgacaagc tgaaccatta ttagtcaatt
     1561 attttttaag caaagaagag ttttctttaa gcagcttgta tgcttttgtt tcgggcattt
     1621 tgcatcaagg tcaagcgcgt gtccttaaac cgagtttttt atttaatggt caattgtacc
     1681 gacaaaccga gcaatggttt aacaaactcc aacaactttg gcaacaacaa ggacgcgaga
     1741 ttagccaact tttttatcaa cacaaacaca cgttaaacgg taataaatat cgggaagatt
     1801 ccgtcactaa atggcttaat ggcctagatt tggctatgag ttctggtttg atggaattat
     1861 tgagcaatga tgatgcgcaa accgccattc acaaactcag ttatagtacg cttcaagagt
     1921 ctttgaaaaa aaatagccct gatttccctc aacatcgctt ttttgatgac tgtgagcatt
     1981 tagccgaatt attagcaaaa accgacaaac aactgcaact acaaggcata gcttttaaat
     2041 gtgatttatt aatccatagt ggcaaagcgt tattagagcg tttacatgcc aaacaagcgc
     2101 aaagttttga tatgcaaatt aaaggcttgg ctaaagcctt aacccatgcc gatacaggtc
     2161 aggtgcttgc ccaagctttg cggcaaa
//
LOCUS       DAOQMB010000395         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_33203_length_2171_cov_9.077032, whole genome shotgun sequence.
ACCESSION   DAOQMB010000395 DAOQMB010000000
VERSION     DAOQMB010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33203_length_2171_cov_9.077032"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLY05_10355"
     CDS             complement(<1..230)
                     /locus_tag="PLY05_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004921413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNC03741.1"
                     /translation="MTTRLTLTRPDDWHIHLRDGVALNNTVADVARYFGRAICMPNLV
                     PPVKTTEDALAYYQRIKAATPSNSSFDPLMVL"
     gene            451..1794
                     /locus_tag="PLY05_10360"
     CDS             451..1794
                     /locus_tag="PLY05_10360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNC03742.1"
                     /translation="MLKETTAPATSIPHPIAALSQNELAHERVAIMTLLANRRRLGER
                     FSDSLEENYREFLVQRAIGLLKANKWIALLFYLFLGLLTYQQVRYVSTFASLEHDLAI
                     WAFIYFGGAFVFSAIGVCVSQAKLDKYYSWYIGIASFIGLTGMSVTASAFDSIYINQQ
                     ASYVVIFIAMLIYSLGSLRIFTTLLIGLFSALLALTIISTIGLPVEYGQFAQYAGLSN
                     VMGFLIASMLDQRDRKGFLQARLIDIEKQQLNQLSQEMARLSQEDGLTGLANRRHFNE
                     TLAREWAIAEREKQSIALIFIDVDHFKPYNDTYGHLEGDKVLSQVGKTLKNMAKRPAD
                     LAARYGGEEFVLLLPNTTIEGAFALAQEVRQAIDDLAIPHKSSKASKCVSVSIGLSAL
                     VPNGQNTISTLIDNADEGVYAAKKAGRHRIRVYPVPEEIAQRKETMMNNLNIKKS"
     gene            1873..>2171
                     /locus_tag="PLY05_10365"
     CDS             1873..>2171
                     /locus_tag="PLY05_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010196371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNC03743.1"
                     /translation="MAEIKKVVLAYSGGLDTSVIVKWLQENYNCEVVTFTADIGQGEE
                     VEPARAKAQAMGVKDIYIEDLREEFVRDFVFPMFRANAIYEGEYLLGTSIARPLIA"
BASE COUNT          687 a          353 c          470 g          661 t
ORIGIN      
        1 tataaaacca ttaagggatc aaagctgctg ttgcttgggg tagcggcttt aatacgttga
       61 tagtacgcca aagcatcttc ggtggtttta acaggtggca caagattagg catacaaatc
      121 gctcgtccaa aataacgagc aacatccgct actgtgttgt ttaaggctac gccatcgcgt
      181 aggtgaatat gccagtcatc aggacgagta agggttaagc gagtagtcat aaagagtcgg
      241 tcaaattaaa agtgaaatta agagaaaaca gaccctaggc atttcactga aattagcacg
      301 ttttgcgagc atgaaatgta agcaaataag cgataattgt ggaagtccta gaaaagtacg
      361 ctatctttag caaaagttga gttattataa cgtaaaaaat catagactct aaacactata
      421 aaaaagccat ctaaaaataa gaggcaaata atgctaaaag aaacaacggc tcctgctacc
      481 agtattcctc atcctattgc agcattgagt caaaatgagt tagcccatga acgtgtggcg
      541 attatgacct tattggccaa tcggcgtcgt ttaggtgaga gattttctga tagtttggaa
      601 gaaaattatc gcgagttttt ggtgcaaaga gccattggtt tattaaaagc caataaatgg
      661 attgcgctgt tattctatct ctttttgggt ttgttaactt atcaacaagt acgttatgtc
      721 tctacctttg cctctttaga acacgattta gccatatggg cgtttattta ttttggtgga
      781 gcatttgttt ttagtgcgat aggtgtgtgt gtttctcaag caaaattaga taaatattat
      841 tcttggtata ttggtatagc gtcatttatt ggtttgacag gcatgagtgt caccgcatct
      901 gcttttgata gtatttatat taatcaacaa gccagttatg tggtcatttt tattgccatg
      961 ttaatttata gtttgggaag tttgcgtatt tttactactt tattaattgg tctattttcg
     1021 gctttattag cgttaacaat tattagcact attggtttac ctgttgaata tgggcaattt
     1081 gctcagtatg caggcttatc aaatgtgatg ggatttttaa ttgcctctat gttagatcaa
     1141 cgcgatagaa aaggtttttt acaagcacgt ttaattgata ttgaaaaaca acaacttaat
     1201 caactaagtc aagaaatggc gcgtttatca caagaagatg gtttaacagg tttggcaaat
     1261 agacgtcatt ttaatgaaac tttggcgagg gagtgggcaa ttgcagaacg agaaaaacaa
     1321 tccattgcct tgattttcat cgatgttgac cacttcaaac cttacaacga cacctatggt
     1381 cacttagaag gtgataaagt gttaagccaa gtaggtaaaa cgctcaaaaa tatggccaaa
     1441 cgtcctgcgg atttggcggc acgttatggt ggcgaagaat ttgtattgtt gttgcctaat
     1501 accactattg aaggggcttt tgcattagca caagaagttc gacaagccat tgatgatcta
     1561 gcaattcctc acaaaagctc aaaagcttct aaatgtgttt cggtgagcat cggtttatcg
     1621 gctttagtgc ctaatggtca aaatacgatt agtacactga tagataatgc tgatgaagga
     1681 gtttatgcag cgaaaaaagc aggaagacat agaattagag tttaccctgt cccagaagag
     1741 attgctcaac gtaaagaaac aatgatgaat aacttgaata taaaaaaatc ctagtgaaaa
     1801 ctctcatagt caatagcacg gtaaatggcg taaaatagga cgtttttttc aaaggcttaa
     1861 aggtaagcaa acatggcaga aattaaaaag gttgttttag cctattcggg tggcttagac
     1921 acatcagtaa ttgtcaaatg gttacaagaa aattacaact gtgaagtggt gacctttacc
     1981 gccgacattg gtcaaggtga agaagtagag cctgcacgtg ctaaagcgca agcaatgggt
     2041 gttaaagaca tctatatcga agatttacgc gaagagtttg tccgtgattt tgtattccca
     2101 atgtttcgtg ccaatgccat ttatgaaggc gagtatttat taggtacgtc gattgcacgt
     2161 ccattaattg c
//
LOCUS       DAOQMB010000396         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_33385_length_2165_cov_16.723223, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000396 DAOQMB010000000
VERSION     DAOQMB010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33385_length_2165_cov_16.723223"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="PLY05_10370"
     CDS             <1..370
                     /locus_tag="PLY05_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03744.1"
                     /translation="KTMKPLFDTFRSHSLEILANLKERYGLLLRRDLVSAKLLHKEGQ
                     LFLEYTVTDKDNYSFDNKVITKSLTLTAEINDALSLQNSIFENARYIVEELPPTSLAQ
                     MADIFDESSAAAIINYAQKS"
     gene            563..1492
                     /locus_tag="PLY05_10375"
     CDS             563..1492
                     /locus_tag="PLY05_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNC03745.1"
                     /translation="MDILAQQLINGLILGSIYALIALGYTMVYGILGLINFAHGEIVM
                     IGAMVCITCLNLLLGHAGDTSPLLLVFASVLMAIPVCAALGYSIERIAYRPLRNAPRL
                     APLITAIGVSIVLQNMAMIIWGRQYVPFPAIFENAPHQVFGASITSLQISIIVLATLM
                     MGALWLLVQKTKLGRAMRATAQSPQVASLMGVNVNQVIAMTFILGSCLGAVAGIMVAA
                     NYGQAHAYMGFLLGLKAFCAAVLGGIGNIAGAVIGGILLGIIESVGAGYLGDLTNNFL
                     GSQYQDIFAFLVLILVLVFRPSGLMGEKMAERP"
     gene            1492..>2165
                     /gene="livM"
                     /locus_tag="PLY05_10380"
     CDS             1492..>2165
                     /gene="livM"
                     /locus_tag="PLY05_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Part of the ABC transporter complex LivFGHMJ and
                     LivFGHMK involved in the high-affinity transport of
                     branched-chain amino acids; LivFGHMK is specific for the
                     transport of leucine, while LivFGHMJ is a transporter for
                     leucine, isoleucine, and valine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HNC03746.1"
                     /translation="MKKIWLGYGLLALGLVFAPFLVGSFGASWVRILDFCLLYIMLAL
                     GLNIVVGYAGLLDLGYIAFYAVGAYVMAILASPHLELHYSAWMLLPFGAFLACVAGVL
                     LGAPTLRLKGDYLAIVTLGFGEIVRLFMNNLDRPVNITNGPQGINMVDNIKMGSYDFG
                     QGFDIAGHSFGSAYSYYYLFLVLALLTIFICIRLEDSRIGRAWMAIREDELAAKAMGI
                     NTRNIKL"
BASE COUNT          537 a          369 c          506 g          753 t
ORIGIN      
        1 aaaaaccatg aaaccgttat ttgatacttt tcgctctcat tctttagaga ttttagccaa
       61 tttaaaagaa cgttatggct tattgttacg tcgtgattta gtcagtgcca aattactgca
      121 caaagaagga caattatttt tagaatatac cgtcaccgat aaagataatt attcttttga
      181 taataaagtg attaccaaaa gtttaacttt aaccgctgaa attaatgatg cgttgagtct
      241 acaaaattct atttttgaga atgcgcgtta tattgttgaa gaattaccac cgaccagttt
      301 ggcacaaatg gctgacattt ttgatgaaag ttcagcagct gcgattatta attatgcgca
      361 aaagagctaa gtcttaaaag tctgtcaggt catagagcat tagtcgattg gcttagtttg
      421 ctacaatacc ccccaaagag gctatgtttt aaaacagagc ttacaacttg taagctctta
      481 gtagtcgtaa cttggcaagc atacagaacg ggcgtattag aaacttatca tacgtcagtc
      541 ttcagtaaag agagttgttg ttttggatat cttagcccaa caattaatta atggcctgat
      601 cttaggcagt atttatgcgt taatcgcgct tggttatacc atggtctatg gtattttggg
      661 cttgattaac tttgcgcatg gcgaaattgt catgattggt gcaatggtat gtattacctg
      721 cctcaattta ttattaggcc atgcaggtga cacctcgcca ctattattag tgtttgccag
      781 cgtgttaatg gccattcctg tgtgtgctgc tttaggttat agcattgagc gtattgcgta
      841 tcgacctttg cgcaatgctc cacgtttagc acctttaatt accgcaattg gcgtatctat
      901 cgtgttgcaa aatatggcaa tgattatttg gggcagacaa tatgtgccgt tccctgccat
      961 ttttgaaaat gcgccacatc aagtgtttgg ggcatcaatt acctcattac aaattagcat
     1021 tattgtttta gcaaccctga tgatgggtgc tttgtggtta ttggtgcaaa aaaccaaatt
     1081 gggtcgtgca atgcgtgcca ctgctcaaag tccacaagtt gcttcgttga tgggtgttaa
     1141 tgtcaaccaa gtgattgcca tgacctttat tttaggctct tgcttaggtg cggtggcggg
     1201 tattatggtt gctgcaaact acggacaagc ccatgcctac atgggctttt tattgggtct
     1261 aaaagccttc tgtgccgcgg tattgggtgg cattggcaat attgcagggg cagtgattgg
     1321 cggtattttg ttgggcatta ttgaaagtgt tggtgcaggt tatttaggtg atttaaccaa
     1381 taacttttta ggcagccaat accaagatat ttttgccttt ttggttttga ttttagtctt
     1441 agtgtttaga ccatcgggtt tgatgggcga aaaaatggcg gagcgaccct aatgaagaag
     1501 atttggttag gctatggctt gctagcatta ggcttagtgt ttgcgccgtt tttggttggc
     1561 tcgtttggcg catcgtgggt gcgaatttta gatttttgtt tgttatacat tatgttggcc
     1621 ttggggctaa atattgttgt tggttatgcg ggtttattag atttaggcta cattgctttt
     1681 tatgcggtgg gtgcttatgt catggcgatt ttggcttctc cacatttaga gctacattat
     1741 tcggcatgga tgttattgcc ttttggtgcg tttttagcct gtgttgcagg ggtgttattg
     1801 ggtgcaccta ccttacgttt aaaaggcgat tatttagcca ttgtcaccct tggttttggt
     1861 gaaattgttc gtttgtttat gaataacctt gaccgtccag tgaatattac caatggcccg
     1921 caaggcatta acatggttga taatatcaaa atgggtagtt atgactttgg tcaagggttt
     1981 gatattgcag ggcactcgtt tggttcggcc tatagctatt attatctgtt tttagtgttg
     2041 gcgttattaa ccatttttat ctgtatccgt ttagaagact cacgcattgg tcgagcatgg
     2101 atggcgattc gtgaagatga attagccgct aaagcgatgg gcattaacac gcgcaatatc
     2161 aaact
//
LOCUS       DAOQMB010000397         2152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_33766_length_2152_cov_8.889843, whole genome shotgun sequence.
ACCESSION   DAOQMB010000397 DAOQMB010000000
VERSION     DAOQMB010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33766_length_2152_cov_8.889843"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLY05_10385"
     CDS             complement(<1..926)
                     /locus_tag="PLY05_10385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNC03747.1"
                     /translation="MSNELSKDHQVTVLAHPLMKQYFESTVNFVPVDFSRSRWSLSLH
                     RDLIRILNQIKPNVIHAQANKAALLVKRIRPFVKCSSRFVATLHNQKDNTRMFEGFDN
                     VIVVSPRLASLVKNTPTTAIYNGVRLPSSSKIVTRQEIAQEFGLDAQTPILIAVGRLV
                     DAKGFDVLIEAAAKAQVQLLIVGDGELKADLQKKINQLQATVVLAGFRQDVLRLMSAT
                     DGFILSSRHEGFAYVFVEALLSKRTIIATSIPMVKDFMPEELIVPIADVDALATKLKW
                     AVSHLSQWQYLMQPSFNKAQNLLTLEAMAAST"
     gene            complement(996..>2152)
                     /locus_tag="PLY05_10390"
     CDS             complement(996..>2152)
                     /locus_tag="PLY05_10390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC03748.1"
                     /translation="GCSTLIAFIRDTKLNQAVIAIILPPRERFRPADAGAVGLTVKDF
                     VLASQYASRSKLVVIGRETYPFANVPYQYVSQSFLGRVLGTTKGYAYDCCQWLKQHRS
                     IAIVEVHNRIPLALFIKKKYPHLKVCLHLHNDPYGMQGAKTQTQRHDLLVSLDRIYCV
                     SHYVRRQLLVNLEEYTAKTQVIYNALALPQKLINLERKQNYIVYVGRFIPEKGVLELA
                     QALALVLPQFPQWKAVFLGAWGFGHAAGKSVYEQSVYTALADIGAQVEFKGHVSQAEV
                     MQTLAISSVVVVPSTCGEAFGRVALEAMSMTNAVVVSHLGALPEIVGDAGVILVKLTP
                     DYLAQQLINLLLHPDKIRSLATAAQQQSQFKFSLATQVAQLDDSRQQFME"
BASE COUNT          650 a          478 c          403 g          621 t
ORIGIN      
        1 aacgtagatg ctgccattgc ctctaaagtc aacagattct gcgccttatt aaaactaggc
       61 tgcatcaagt actgccattg ggacagatga gagactgccc actttaactt agttgctaat
      121 gcatcaacat ctgcaatagg aacaatcagc tcttctggca taaaatcctt taccatagga
      181 attgaagtcg caattatggt tcgtttagac agtaatgctt cgacaaaaac ataagcgaac
      241 ccttcatgac gggaactcaa aataaaccca tcagtagccg acattaaacg tagtacatct
      301 tgcctaaaac ccgccaaaac gactgttgct tgtaattggt taattttttt ctgtaaatct
      361 gctttgagct caccatcccc cactattaac aattgtactt gtgcctttgc tgccgcctct
      421 attaacacat caaatccttt tgcgtcaact aagcgtccga cggctatcaa gatgggggtc
      481 tgcgcatcta agccgaactc ttgagcaatc tcttgacgag ttacgatttt tgaagacgat
      541 ggtaaacgaa cgccattgta aatggcagta gttggcgtat ttttgactaa tgaggcaagt
      601 cgaggactaa ccacaatcac attatcaaag ccttcaaaca tacgagtgtt gtccttttga
      661 ttgtgtaaag tagctacaaa tcgactactg cacttaacaa aaggacgaat tcgctttacc
      721 aataaagcag ctttgttggc ctgtgcatgg ataacatttg gcttaatttg attaagaatt
      781 cgtattaaat ctctatgtaa acttaatgac cagcgtgagc gagagaagtc tacaggcaca
      841 aaattaacag tactttcaaa atactgtttc atgagggggt gagcgagcac cgtcacttga
      901 tggtctttac ttaactcatt actcaattca atgacgtgct tttctaaacc tccatgttcc
      961 caactggcaa caatttggca aatatgcata aggctttact ccataaactg ttgacgtgag
     1021 tcatctaatt gagcaacctg cgttgctaaa ctgaatttga attgcgactg ctgctgagct
     1081 gctgtcgcaa gggaacgtat tttatctgga tgcaagagca aatttatcaa ttgttgtgcc
     1141 aaataatcag gtgttaattt gaccaaaata actcccgcgt caccaacaat ctcaggtaaa
     1201 gcgcctaaat gcgataccac aacagcattt gtcatactca ttgcttctaa cgcaacacga
     1261 ccaaatgctt cgccacaggt tgatggcaca actacaacag aagatattgc taaagtttgc
     1321 atcacttctg cttgtgatac atgaccttta aactcgactt gtgccccaat gtcagccaac
     1381 gctgtatata cagattgctc atacacactt ttacccgcag catgaccaaa cccccaagca
     1441 cccaaaaata ctgccttcca ttgtggaaac tgcggcaata ccagcgctaa ggcttgtgct
     1501 agctcaagta cccctttttc gggaataaaa cgtccaacat atacaatata attttgcttt
     1561 cgttcaagat ttattagttt ttgtggcagt gctaaggcat tataaataac ctgtgttttt
     1621 gctgtatatt cttctaagtt tacgagaagc tgacgacgca cataatgact gacgcaataa
     1681 atcctatcaa gagacactaa taaatcgtgc cgttgtgttt gcgtttttgc accttgcata
     1741 ccatatggat cattatgcag atgtaaacac acttttaaat gtgggtattt tttttttata
     1801 aataatgcta aagggatgcg attatgtact tcaacgatgg ctatcgaacg gtgttgctta
     1861 agccactggc aacaatcata ggcataacct ttagttgtac ccaatacacg ccccaaaaac
     1921 gactgtgata catattggta aggtacgtta gcaaaaggat atgtttctcg gcctatgact
     1981 actaactttg aacgtgacgc atactgactt gccaacacaa agtctttgac tgttaatcca
     2041 acagcccctg catctgcggg gcgaaaccgc tcacgaggag gcaaaataat tgctataact
     2101 gcttgattca attttgtgtc tctgataaag gcaataagcg ttgagcatcc at
//
LOCUS       DAOQMB010000398         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34328_length_2132_cov_5.313914, whole genome shotgun sequence.
ACCESSION   DAOQMB010000398 DAOQMB010000000
VERSION     DAOQMB010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34328_length_2132_cov_5.313914"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..949)
                     /locus_tag="PLY05_10395"
     CDS             complement(242..949)
                     /locus_tag="PLY05_10395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNC03749.1"
                     /translation="MNTEQLTINDLTIDVVRKNIKNLHLGVYPPHGHVRVAVPLYIDN
                     EAVRLAIVSRLGWIKRQQAKFDKQPRLSAREYITGESHFVQGRRYLLNVIYHAAAPKV
                     ILQHTTLDLYVRHGSDTAKRQAVLQAWHRQQLKTQIPDLIAKWQPIMGVEVADWGVKQ
                     MKTRWGTCNIRDRRIWLALELAQKPLHCLEYVVVHEMTHLLERHHNDRFKAYMTKFLP
                     NWQSIKHELNQLPLVTA"
     gene            complement(939..>2132)
                     /locus_tag="PLY05_10400"
     CDS             complement(939..>2132)
                     /locus_tag="PLY05_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
                     /protein_id="HNC03750.1"
                     /translation="LLIVVDKLLTGFDAPSATYLYIDKQMRDHGLFQAICRVNRLHGE
                     DKEYGYIIDYKDLFKSLESTFKDYTSGALDAFDAEDVAGLLSDRLDKAKERLEEAREG
                     IKALCEPVALPRSTNDYLHYFCATDSGNVEQLKNNAPKRLMLYKMTAALIRAFANLSG
                     EMEKAGYSPNDIHTIKQEVEHYDNVRTAIKLQSADYIDLKVYEPAMRFLIDTYIRAGE
                     SEKISAFDEVSLVQLMVQKGSQALDSLPDNLRTNPEAVAETIENNVRKLIIDEQPINP
                     KYYEQMSDLLDKLIEQRKQEAIEYQAYLAQIIELARQAVHPNTTSYPQSLNSAAQRAL
                     YDNLSHNEALAIAVHNTVMQTKKDDWRNNTGKTKEVRRAIKEVLPDKTLLDTVFSLVQ
                     NQHEY"
BASE COUNT          558 a          435 c          460 g          679 t
ORIGIN      
        1 gcaccacctt gacatggtgg gggtcgctgg ttcgagtcca gttacgtcta ccaaatttta
       61 tatataaatc agccgcttac aatagcggct tttttatttc tgtgtcacaa aataaaacgt
      121 gtcaccatag tgtcacctag ctcaagaaaa gttgagtttc taaaaactcg aattttttgt
      181 attgaccaat gccatacgca actttattta ttggtttgta ggcatcttgg ttagtcagta
      241 ttcacgctgt aactaagggt aattggttta attcgtgttt gatgctttgc caattgggta
      301 gaaatttagt catatacgcc ttaaagcggt cgttatgatg gcgttctaaa agatgcgtca
      361 tttcatgcac aacgacatat tccaaacaat gcaaaggttt ttgcgctaac tctaaggcta
      421 gccaaatacg gcggtcacga atgttgcacg ttccccagcg tgttttcatt tgttttacgc
      481 cccaatcggc aacttcgact cccatgatgg gctgccactt ggcaattaaa tcgggtattt
      541 gagttttgag ttgttgccta tgccatgctt gtaacaccgc ttgacgtttg gcggtatcac
      601 tgccgtgacg gacgtataaa tcaagggtgg tatgttgcag aatcacttta ggagcagccg
      661 catgataaat cacatttagc aaatagcggc gaccttgaac aaaatgactt tcccctgtga
      721 tgtattcacg ggcggaaagg cgcggttgtt tatcgaattt ggcttgttgg cgtttaatcc
      781 agcctaagcg tgacacaatc gccaagcgaa ctgcttcatt atcaatatac aatggcacag
      841 caacccgaac atggccatgt ggtggataca cacccaaatg caggtttttg atgtttttac
      901 gcaccacatc aattgtcaga tcgttaatgg tcagttgttc agtattcatg ttgattttgt
      961 actaagctaa aaacagtatc caatagggtt ttgtcgggca atacctcttt aatcgcacgt
     1021 ctgacttctt tggttttacc tgtattattg cgccagtcgt cttttttggt ttgcatgacc
     1081 gtattgtgaa cggctatagc taaggcttca ttatggctaa gattatcgta taacgcacgt
     1141 tgagcagcac tgttaagact ttgaggatag ctagttgtat tggggtgaac agcttgtctg
     1201 gccagctcaa taatttgggc aagataggct tgatactcaa tagcctcttg tttgcgttgt
     1261 tcgattaact tgtctaataa atccgacatc tgttcgtagt atttggggtt aattggttgc
     1321 tcatcaataa tcaatttacg aacgttattt tcgatggttt cggcaacagc ttcaggattg
     1381 gtgcgtaggt tatcgggtag ggagtcgagg gcttgactgc ctttttgtac cattaattgt
     1441 accagtgaca cttcatcaaa cgcagagatt ttttcgcttt cacctgcccg aatataggtg
     1501 tcaattaaaa aacgcatcgc aggttcatac acttttaggt caatatagtc ggcactttgc
     1561 agtttgatgg cggtacgcac attatcgtaa tgctcaactt cctgcttgat ggtgtgaatg
     1621 tcgtttgggc tataacctgc tttttccatt tcgccactca gattggcaaa agcacgaatc
     1681 aaggcagccg tcattttata cagcattaat cgtttaggtg cattgttttt gagttgctca
     1741 acattgcctg agtctgtggc acaaaaataa tgtaaataat cgttggtgct acgaggcaag
     1801 gcgacaggct cacataaggc tttaatgcct tcacgagctt cttcgagtcg ttctttggct
     1861 ttatctaagc ggtcgcttaa taatcctgcc acatcttcag catcaaacgc atctaatgca
     1921 ccagaggtgt aatctttaaa ggtactttct aggcttttaa agaggtcttt gtagtcaata
     1981 atataaccat attctttgtc ttcaccatgc aaacgattaa cccgacaaat ggcctgaaac
     2041 aagccgtggt cacgcatttg tttgtcgata tacaaataag tagcagaggg tgcatcaaaa
     2101 cctgttaata atttatcaac cacaattaac ag
//
LOCUS       DAOQMB010000399         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34335_length_2132_cov_4.050554, whole genome shotgun sequence.
ACCESSION   DAOQMB010000399 DAOQMB010000000
VERSION     DAOQMB010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34335_length_2132_cov_4.050554"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PLY05_10405"
     CDS             <1..404
                     /locus_tag="PLY05_10405"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNC03751.1"
                     /translation="FNTITPSSLPTIQVKDKRIKLLLTSPAIFGKGSQLPSLEDKQDG
                     RIYFKGGSAKVVAASIPRHHVISGWDLANWQPKPAERVVPTGAVYWLDDVQYNGTSLQ
                     TALQDLLLCHLDPQRKAEGYNACLIANWTNP"
     gene            426..1355
                     /gene="cmr4"
                     /locus_tag="PLY05_10410"
     CDS             426..1355
                     /gene="cmr4"
                     /locus_tag="PLY05_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HNC03752.1"
                     /translation="MFTAQKTLFLYAVSPVHMGAGQAIGLIDNPIQREVHTGHPVFAG
                     SGIKGAVRHRFYSLLPQTDNRLTRYFGAESDKASDYAGAVSFSDAQLVLFPVRCTKAG
                     YVYATSPLALARAKRLLQQSGLDTWNDLTSPTTNHCLVTQEHLLVEKINDKGQRNKML
                     YVEAYELNAKPDEKLKAIATILANYALSDDQDFFKHKIISDLVLMTDDDFGYFVKNAT
                     SIEPHVRIVPETGVAKDGGLFYTENLPPESVMIQNVMATAERSKAGTDKAEDVLAFVL
                     DGDLGLNGKLLQVGGDATTGRGQVLVNVAGGAK"
     gene            1352..1750
                     /gene="cmr5"
                     /locus_tag="PLY05_10415"
     CDS             1352..1750
                     /gene="cmr5"
                     /locus_tag="PLY05_10415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HNC03753.1"
                     /translation="MSQTLDQKRAAFVWQQLAPLKQRNPAVFDAYTKLSKGAGTLIMQ
                     NGLMPTLAFYGEKSKGSASLAGGQEHELLLAHLLAWLNQQQLLSTASFAPAMAALLTK
                     TSPEYRAITEECLALIRWIRHFASALNKTE"
     gene            1756..>2132
                     /locus_tag="PLY05_10420"
     CDS             1756..>2132
                     /locus_tag="PLY05_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03754.1"
                     /translation="MGQAATPRYMPDRNDAFYAELSPALAFYTFFPIWDNSWRLDGHY
                     KSDALKKVLTYSKSAQAQTTALIERQKNLISKTDIKHRLSITAKSISPFMTGTGMEHP
                     LENGFAFLQPYGLPYLAGSSIKGV"
BASE COUNT          654 a          443 c          430 g          605 t
ORIGIN      
        1 ggtttaatac cataacacct tcatcattac caacgattca agttaaagat aaacgcatta
       61 aattattact cactagccct gctatttttg gcaaaggttc acaattacct agcctagaag
      121 ataaacaaga tggccgaatt tattttaaag gtggctctgc caaagtggta gctgccagca
      181 ttcctcgtca tcatgttatt tcgggatggg acttggctaa ttggcagcca aaacctgctg
      241 aacgtgttgt accaacaggt gcagtatatt ggctagatga tgttcaatat aacggcacaa
      301 gcttgcaaac agcactacaa gacttacttt tgtgtcatct tgacccacaa cgcaaagcag
      361 aaggctataa cgcttgccta atagccaact ggacaaatcc ataatttgta ttaaaggaaa
      421 tttttatgtt tactgctcaa aaaactttat ttttatatgc tgttagccct gtacacatgg
      481 gcgcaggtca agcaattggc ttaattgata atccgattca acgcgaagta cacacaggcc
      541 atcctgtttt tgctggctct ggcattaaag gtgctgtacg ccatcgtttt tatagcttat
      601 tacctcaaac cgataaccgt ttaacccgtt attttggtgc tgaatctgac aaagccagtg
      661 attatgcagg ggctgtgagt tttagtgatg cgcaattagt gttgtttcct gtgcgttgta
      721 ccaaagcagg ttatgtatat gccaccagtc cattggcgtt agcgcgcgcc aagcgtttat
      781 tgcaacaaag tggcttagat acttggaatg atttaaccag tcctactact aatcactgtt
      841 tggtgacaca agaacatttg cttgtagaaa aaataaatga caaaggccaa agaaataaaa
      901 tgctttatgt tgaagcctat gagttaaatg ccaagccaga tgaaaaactc aaagcgattg
      961 ccacaatatt ggctaattat gctttatcag acgaccaaga cttttttaaa cacaaaatca
     1021 tcagcgattt ggtgctaatg actgatgatg attttggcta ctttgtaaaa aatgctacca
     1081 gtattgagcc tcatgtacgc attgtgccag aaacaggtgt cgccaaagat ggtggtttgt
     1141 tttataccga aaacttaccg cccgaaagtg tcatgattca aaacgtcatg gctacagcag
     1201 agcgcagcaa agcaggcaca gacaaagccg aagatgtttt agcttttgta ttagatggtg
     1261 atttaggctt aaacggcaaa ttattacaag taggtggtga tgctactaca ggacgcggcc
     1321 aagtattagt caatgtggca ggaggtgcaa aatgagccaa actttagacc aaaaacgtgc
     1381 agcctttgtt tggcaacaac tcgctccttt aaagcaacgt aatccagctg tttttgatgc
     1441 ctataccaaa ctttctaaag gtgcaggcac attgattatg caaaatggtt taatgccaac
     1501 cttagctttt tatggcgaaa aatcaaaagg gtcagcctca ttggcaggtg gacaagaaca
     1561 tgagttatta ctggctcatt tattggcatg gctcaatcaa caacaattat tatcaacagc
     1621 atcattcgcc ccagcaatgg cagcactact cactaaaacc agtcccgaat atagagccat
     1681 taccgaagaa tgtttggcgt taattcgctg gattcgtcac tttgccagtg ccttaaacaa
     1741 aacggagtaa ttaacatggg acaagctgcc accccacgct atatgccaga ccgtaatgat
     1801 gccttttatg ctgagctttc gccagcacta gcgttttata ctttttttcc tatttgggat
     1861 aactcttggc gactagatgg acattataaa agtgacgcat taaaaaaggt actcacttat
     1921 tcaaaatccg cccaagcaca aaccacagcc ttaattgaac gccaaaaaaa cttaattagc
     1981 aaaacggaca tcaagcatcg tttgtctatt accgctaaaa gtatcagtcc ctttatgaca
     2041 ggtactggca tggagcatcc gttagaaaat ggctttgctt ttttacagcc ttatggcttg
     2101 ccttatttag caggcagcag tattaaaggt gt
//
LOCUS       DAOQMB010000400         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34444_length_2128_cov_16.363242, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000400 DAOQMB010000000
VERSION     DAOQMB010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34444_length_2128_cov_16.363242"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..610)
                     /locus_tag="PLY05_10425"
     CDS             complement(113..610)
                     /locus_tag="PLY05_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC03755.1"
                     /translation="MKVALNTNFGRIVLELDEVNAPKTSANFVSYVKSGHYNGTIFHR
                     VIPNFMIQGGGFEANMNQKSTKAPIENEANNGLKNDVGTIAMARTQDPHSASAQFFIN
                     TKKNDFLNHSSKTVQGWGYAVFGKVVEGMDVVTAIEKVRTGNKGYHGDVPVEPVVIET
                     AEVVE"
     gene            766..2128
                     /locus_tag="PLY05_10430"
                     /pseudo
     CDS             766..2128
                     /locus_tag="PLY05_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803942.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
BASE COUNT          616 a          409 c          448 g          655 t
ORIGIN      
        1 gtagcgtgtg gttgacgtag gaaacccacg attcacattt aattatcgtg ggtttcgttc
       61 cttaacccac gctacgctta ctgggcaaaa tgcccagctc attatttata aattactcaa
      121 caacttctgc tgtttcaatg acaactggct caacaggcac atcaccatga taacctttgt
      181 tgccagtgcg tactttttca atagctgtaa caacgtccat accttcaacc actttaccaa
      241 ataccgcata accccaacct tgaacggttt tgctggaatg gtttaaaaag tcattctttt
      301 tagtgttaat aaaaaattga gcagaggcag agtgtggatc ttgagtacga gccatcgcaa
      361 tcgtgccaac atcatttttt aaaccgttgt tagcttcgtt ttcgatagga gctttagtag
      421 atttttgatt catgttggct tcaaaaccac caccttgaat cataaagttt ggaataacac
      481 ggtggaaaat agtgccgttg tagtgaccac ttttaacata gctaacaaag ttagcagatg
      541 tttttggtgc gttaacttca tctaattcta aaacaatacg accgaaatta gtatttaatg
      601 caactttcat tctcgtttcc tagtttgttg agggtttact acttgagact aggctaagca
      661 atcgctagac ttgtcttttt gatatttgca cctaagcatt atagcaagtg cgtttactag
      721 aagctaattt acgctaatca tatgagttgt ttaatggaag ccaaagattt tattcgtgca
      781 caaattgttg aagatttggc cgcaggcaaa catgccaaag ccattacccg ttttccgcca
      841 gagcctaatg gttatttaca cattggccat gccaaatcaa tttgtttgaa ttttggtatt
      901 gcggtcgaga gtcagggtga atgtaatttg cgttttgatg acactaatcc cgaaaaagag
      961 tcgcaggaat atattgatgc cattaaacgt gatgtcgagt ggatgggttt taattgggca
     1021 ggcgatgttc gttatgcctc caattatttt gatgcattgc atgattatgc cattgcctta
     1081 attaaaatgg gcaaggcgta tgtagactca caaacacccg aacaaattca acaaacgcgc
     1141 ggtagcttta gtgaagcagg taaaaactca ccctatcgtg atcgtagtgt tgaagaaaac
     1201 ttagcacttt ttgaaaaaat gcgtttgggt gaatttgccg aaggtgagtg tgtgttgcgt
     1261 gcaaaaattg atatggcatc aggcaatatt aatttacgtg accctgtttt atatcgcatt
     1321 aaaaaagtgg cacatcatca aacaggggat aaatggctga tttatcctat gtatgattat
     1381 acccacccaa tttcggatgc cttagaaaac atcactcatt ctttgtgtac tctagagttt
     1441 gaagatcatc gaccttttta tgattggttg ttagacaccc tacaaacgcc agcgcatcca
     1501 caacaaattg agttttctcg tttaaatctc aattacaccg tcaccagtaa gcgtaagctg
     1561 aaaaaattgg ttgatgaaaa aattgtcaat ggttgggatg atcctcggat gccaaccatt
     1621 gcaggtttgc gtcgtcgtgg ttatacacct gaatcattac gcgatttctg tgagcgcatt
     1681 ggcatcacca aaagtgatgg tgtggttgat tttagcttat tagagttttg tatccgtaac
     1741 cacttagaaa atacagcacc acgtgctatg gcggtgttaa atccgttaaa agtagtgatt
     1801 accaatgttg aagaggcaac cttagcctta tcttcagcac gtcatcccaa cttacctgaa
     1861 ttgggtgagc gcgaaattcc gtttaccaaa gaaatcttca ttgatgccgc agactttagc
     1921 gaaaacccac ccaaaggtta taaacgtttg gtagtcggtg gcgaagtgcg tttgcgtcac
     1981 tgttatgtga ttcgttgtga tgaagtggtc aagaacgaag caggtgagat tgttgagcta
     2041 cgttgttctg ccgatttaaa tactttgggc aaaaatcctg aaggacgtaa agttaaaggc
     2101 gtgatccaat gggtatcagc tacattag
//
LOCUS       DAOQMB010000401         2125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34540_length_2125_cov_4.603865, whole genome shotgun sequence.
ACCESSION   DAOQMB010000401 DAOQMB010000000
VERSION     DAOQMB010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34540_length_2125_cov_4.603865"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(134..886)
                     /locus_tag="PLY05_10435"
                     /pseudo
     CDS             complement(134..886)
                     /locus_tag="PLY05_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            1087..2052
                     /locus_tag="PLY05_10440"
     CDS             1087..2052
                     /locus_tag="PLY05_10440"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNC03756.1"
                     /translation="MAIAFDSKAKASSNYRRIQRFIHDRKLIDFNHVSRFIMTLFGFL
                     EQDYYLTFDRTNWKWGKKNINILLLAIAYKGIAIPIYWLLLNKKGNSSTRERIALLKR
                     FTTQFGKKRIIRFLADREFVGEEWFNRLKSENIDFVIRIKKNNLISNSRGDLLPAEQL
                     FRHLRVGESMILVGARQAYNTSVFVSALRLKDGELLIVVTGRPCTDAIDTYARRWQIE
                     TLFSCLKSRGFNFEDTHVTGRCRIKSLLVVLVIAFCWAHRVGEWTHDNVCSIKIKKHK
                     RPEKSIFRVGLDVISECLFKSKHSRKTKLNSLFLHLKPTLESSHE"
BASE COUNT          633 a          438 c          429 g          625 t
ORIGIN      
        1 ttggtaactc tcttcttttc aagtcctttt gtcagattaa gtctgaacta atacagctaa
       61 gttgtaatgt ccgcttttgg gctaagttga aatgtccata ttttatttaa cttagccatt
      121 agtatctgag tttttacgta caaatcccta catttaattg attaattgcg ttctgtatat
      181 ctgtttcatt caaagctaat ggcggttttt tacctaaagc tttaccacgt tttcgtattt
      241 ccccataatt tgcgggaacc gattgcgagc gttgattatc acgctgcgct tgtactaatt
      301 gcgccatctg taaaacatga cccaaacgct tattatcaac aatcgtcccc tgatcaactt
      361 ccgataagcg atcataaatc gtgaagggca gggaaacacc atttgctcga ggctcaatcc
      421 gcccatcagc gtaatgatat acatcaatat agcacccaac caatgcacga gccgatggcg
      481 tatcttctaa caaataaagc cgtttatcat attgcagcgt taaacttttc gatacacaac
      541 gaggctcacg ccatgcaaaa atcaaatcaa gattttcatc ctgccttaat ggcctatgca
      601 catcatgatc acttctcggc ggcttagcaa agcgagcgtt atagtccgcg ataaactcag
      661 gcatgaacgc attagcctct gtcatagtgc tgatcccacg caatctcaac tccttaacta
      721 atcgatcttg tagcgtcaga ttagctcgct caacacgccc tttagcttgg ctggaattcg
      781 cacaaatccc ttcaatattg agttcgtaga gtgcgcgacc aaactgtgta tgaccatcac
      841 ctccacgtat gtccttatta ttaacacgaa aaacactagc tttatcctct gtacatgaca
      901 aaaaagaaaa acccgttgaa aatggggcat aatattgaat ttttgcgaaa cctaaaacca
      961 tgcaacactt caacgagcta gccgttattc taaaccagca ttttaaatgg aacaaggcgc
     1021 gcatgaattg ccttgtctta atggtgatag ccttgttttt acaaggagac gacaaacctc
     1081 agcaaactgg cgattgcttt tgatagcaaa gcgaaagcga gttctaacta tagacgaata
     1141 caacgcttta tacatgatag gaagctgatt gattttaatc atgtttcaag atttatcatg
     1201 acgctatttg gttttttgga gcaggactat tacctcacgt ttgaccgtac caattggaag
     1261 tggggcaaga aaaacatcaa catactcctg cttgcgattg cttataaagg gattgccata
     1321 ccgatttact ggctgttgtt gaataaaaag gggaactcaa gcacgcgaga acggattgcg
     1381 ctactgaagc gctttaccac acaatttggc aagaagagga ttatcaggtt tcttgctgac
     1441 cgtgagtttg tgggtgaaga atggtttaac aggctaaagt cagagaatat tgactttgtg
     1501 attcggatca agaaaaacaa tcttatcagc aacagtcgag gcgatttgct ccctgctgag
     1561 caactatttc ggcatttaag agtgggtgaa agcatgattt tggtaggcgc aagacaagcc
     1621 tacaatacct ctgtttttgt atcggctttg cgattgaaag acggtgagct attaattgtt
     1681 gtaacaggtc gtccttgcac ggatgccatt gatacttatg caagaagatg gcaaatagag
     1741 acattattta gctgcttaaa aagtcgggga ttcaactttg aagacactca tgtcactggg
     1801 cgttgtcgga ttaagtcgtt acttgttgtg ctggtaattg ctttttgttg ggcgcatcgt
     1861 gtgggtgaat ggacgcatga taatgtgtgc tctattaaga taaaaaaaca taaacgccct
     1921 gaaaaaagta ttttcagggt gggattggat gtcattagtg agtgtctttt taagtcaaaa
     1981 cacagcagaa aaacaaaact taactccctg tttttacatc taaaaccaac acttgaaagc
     2041 tctcatgaat aatttttgtc atgtacagag aaaatcacct agacctgctg cattttcagg
     2101 ggcaataacg agcacttgtt caaaa
//
LOCUS       DAOQMB010000402         2119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34710_length_2119_cov_28.819283, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000402 DAOQMB010000000
VERSION     DAOQMB010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34710_length_2119_cov_28.819283"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(513..1670)
                     /locus_tag="PLY05_10445"
     CDS             complement(513..1670)
                     /locus_tag="PLY05_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03757.1"
                     /translation="MALASLYVKRGLSSRELSRQLAHSKLTGHDCALLRPFAEKTNFT
                     LSELKAFDGKPSCRPKYFFPLASQWLSKADKLALGVGVTRIGSPCRASAPLPCLRINN
                     SNDSPLTAPKLVNPEPVIVPSWVRKSEFKVKTEVMALVARYGIEHIGFLTFTFADDVQ
                     CHKEATKRLNSLITGVLRDRYIKAIRVSERQNSGRWHFHLVIVCKQDIRRGVDFQAIK
                     ERRYKDGANSALRSEWAFWRKTSKKYGFGRTELLPIRKTGEALAAYVAKYISKGFAYR
                     QQQDKGARLYSVIGGDRVCSSRFSFHSLKTFLWRKKVDMWAASWSSALARVALSGGHA
                     PVDALNYDDLPSLFGKRWAWRYRDDIMAMMPVLTPEQFNHFIAFNPSILPH"
BASE COUNT          666 a          459 c          438 g          556 t
ORIGIN      
        1 gcaacctttc acttgtttat gttgttgtgg ggttgtgcca gttaatttta agttatacat
       61 catcgctcaa atctaataga accctccgaa ttattcaaga cctgataata cccatcgtta
      121 aaataaaatg attatttcta tttaatacaa agtaaatagt aataagtatt aagtaataag
      181 tgttatttaa tataatttta agggttgatt aaggtgcggt taattgactt ttattctaac
      241 gatataatca ccgctcatta ggtattgcat attaataaat acgcatggtt aatcgcgcat
      301 ctagccttta agagttattt tggtagagac gatgattaaa gctataggct acgcaccgcg
      361 ctttgtcgtt gtcgttccct ccgcctccgc gcttggtgct tgcttttgac tgtttgataa
      421 aataataaag taagataaac gataataaag taaagtaata aaataagata aaagataacg
      481 taatcaataa gataagttat taaattatct tatcaatggg gtaagataga cggattaaag
      541 gcaataaagt ggttaaattg ctcaggagtc aagacaggca tcatagccat aatgtcatca
      601 cgataccgcc aagcccatcg cttaccaaac aaagacggca aatcatcata attcagggca
      661 tcaacagggg catgaccacc cgacaaggca acacgggcaa gggcagaaga ccacgaagcc
      721 gcccacatat cgactttttt acgccaaagg aaggttttaa ggctatggaa cgagaaacga
      781 gacgagcaga ccctatcacc gcctatcact gaatagagcc ttgcgccctt gtcttgttgt
      841 tgcctataag cgaagccttt agaaatatac ttggccacat aagcagctaa cgcctctcca
      901 gttttacgaa tagggagcaa ctcagtacga ccgaaaccat actttttaga agttttgcgc
      961 cagaacgccc attcagaacg caaagcagaa ttagcaccat ctttataacg ccgctcttta
     1021 attgcctgaa agtcaacacc gcgcctaata tcttgtttac aaacaatgac taagtgaaaa
     1081 tgccaacgcc ccgaattttg acgctcagag acccgaatcg ctttaatata cctatcacgc
     1141 aagacacccg ttatcaacga attaaggcgt tttgttgcct ctttatggca ctggacatca
     1201 tcggcaaatg tgaaagttaa gaatccaata tgttcaatac cataacgcgc gactaaagcc
     1261 atgacctcag tcttgacctt aaattcagac ttacgaaccc acgatggcac aatgacaggt
     1321 tcaggattca ccaattttgg tgcagtgagt gggctatcgt ttgagttgtt tattctaaga
     1381 caaggaaggg gcgcagacgc gcggcaggga ctgccgatgc gcgtcacacc cacccccaaa
     1441 gctaacttat cagccttgct taaccattga gaagcgagcg gaaagaagta tttaggacga
     1501 catgaaggct tgccatcaaa ggcttttaac tcagataggg taaaattggt tttttcagca
     1561 aagggacgca ataacgcgca atcatggcct gttagcttgc tatgagctaa ttgtcgggat
     1621 aattcacgag aggagaggcc acgcttaacg taaagagacg ctagagccat cttagccgcc
     1681 ttgcaaacgc aagacagcaa aaacttaata aggcaagccg tagggactta ccaagcagcc
     1741 aaaaagccgc tagaatagtt ttcatcagtc tagtatccgc ctgtgaaacg aatttgtaga
     1801 ctggtcatgg cgtgaagtgt tccaccactt gcacgccatt tttattggcg cactaagtta
     1861 gcagaaccac accgaacggc atagtttgca taatcactcc ttacagcaca ttctagggta
     1921 ggttgctacg accttaacca acgaagacgg caattgtgta atttttgagt ttaaataata
     1981 gagcctaata gaccagtaat ccttgttagc agcctcttta cagtgctgcc gcgctgatgt
     2041 ataacttgtc gttcaagcgg acacgaactc gggcggagtt aataatttat attaaccgcg
     2101 tgccgcttaa ctcgtcgtt
//
LOCUS       DAOQMB010000403         2112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34925_length_2112_cov_9.629071, whole genome shotgun sequence.
ACCESSION   DAOQMB010000403 DAOQMB010000000
VERSION     DAOQMB010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34925_length_2112_cov_9.629071"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1119)
                     /locus_tag="PLY05_10450"
     CDS             complement(97..1119)
                     /locus_tag="PLY05_10450"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNC03758.1"
                     /translation="MTNVISSHDFSLYLHELLQAKAQQQVEPLRLLVAQYQGVLAHFS
                     HLQEEVNKLLTPQTGRDYSLVAVALKQGLQELEKLLNGLIKAHDDLLLSVVEVDDISA
                     LAELFSCRIAQQVLQKTSTARRKQLDKQKHKLLASETESKDLVKEDLLATIVDQTNDF
                     VAQTKEDRMLNKLEAQFSGWRKIGASGEELGVLAASWAAVVDEKTKLMWAINTNLIEK
                     YPNCSDLMWFYPELKGGEGFANQGKNTTDWLRYVNTKGWCGFYDWRLPTLEELRGLLT
                     TEVSRYYHIREDIFTDMQALGSRFWSSTVDHKSTEYAWAMYFGYGHDGLAHKSYSLNV
                     RLVRDI"
     gene            complement(1112..1873)
                     /locus_tag="PLY05_10455"
     CDS             complement(1112..1873)
                     /locus_tag="PLY05_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03759.1"
                     /translation="MITKQSLALDQPIEKQQLPLFWRLGKLNTGQPLLWPINYQQPII
                     IKCVHKTHETAHRFLETLLLSLWQQTGGQQLQIVLYEASLKRVFHYSSQLPVQVLYHK
                     QHVKKHIADLHALAQQRQDILKQLNYVSWFDYINQQSQVEPLQLVVLSQLWPDPELLA
                     DLSDLCRYGGQFGILPVLVVSEHFFPAIADDWYAQFLEECSLKALVVELKQGYYVQIK
                     HHQLQEIADLYAFFKPKIDRYSESVLTAVCRGEYD"
BASE COUNT          676 a          440 c          326 g          670 t
ORIGIN      
        1 gaactcacgc gccaacaacg tattgaagaa attgcacgaa tgtcaggtgg tttaacgatt
       61 actcaagaaa ccttaaaaca tgccgaaagt atgttatcaa atatctcgga ctaaacggac
      121 attaaggcta taacttttat gtgctaagcc atcatgtcca taaccaaaat acattgccca
      181 agcatattct gtacttttat gatcaacagt tgatgaccag aaacgacttc ctagagcttg
      241 catatccgta aaaatatctt cacgaatatg ataataacga gacacttccg ttgtgagtaa
      301 gcctctcaac tcttctaaag ttggtaaacg ccaatcataa aaaccacacc aacctttggt
      361 attcacataa cgtaaccaat ctgtagtatt tttaccctga ttggcaaacc cttctcctcc
      421 ctttaactca ggataaaacc acatcaaatc actacaattg ggatattttt ctattaaatt
      481 cgtattaatt gcccacatca attttgtttt ttcatcaaca acggcagccc aactagcagc
      541 caaaacacct agctcctcac ctgatgcacc aattttgcgc caaccactaa attgggcttc
      601 taatttattt agcattctgt cttctttagt ttgggcaaca aaatcattag tctggtcaac
      661 tattgttgct aaaagatcct ctttcactaa atctttcgac tctgtttcac tcgccaaaag
      721 cttgtgtttt tgcttatcta attgtttacg acgtgcagtt gatgtttttt gtaatacttg
      781 ctgagcaata cgacagctaa ataactcggc taaggcacta atatcatcaa cttcaaccac
      841 acttaacaat aagtcatcat gggctttaat taagccatta agtagttttt ctagctcttg
      901 taaaccttgt ttaagcgcaa ctgcaactaa actataatca cgtccagttt gtggtgtaag
      961 caatttattt acttcttctt gcaaatgtga aaaatgagcc aataccccct gatattgggc
     1021 aactaacaga cgtaatggtt caacttgttg ttgggcttta gcttgcagta attcgtgtaa
     1081 ataaagacta aagtcatggc tagaaatcac gttagtcata ctcccctcga caaactgctg
     1141 ttaataccga ctcagaataa cgatcgattt ttggcttaaa aaaagcatat aaatcagcaa
     1201 tttcttgtaa ttgatgatgt ttaatctgta cataatatcc ctgttttagc tcaacaacta
     1261 aggcttttaa actacactct tcaagaaatt gtgcatacca atcatccgca attgcaggaa
     1321 aaaaatgctc tgatactact aaaacaggca aaataccaaa ttggccacca taacgacata
     1381 aatcacttaa atctgctaat aactctgggt ctggccatag ttgactcaac accactaatt
     1441 gtagcggttc tacttgggat tgctgattta tataatcaaa ccatgagaca taatttaatt
     1501 gttttaaaat atcttgacgc tgttgagcaa gtgcgtgcaa atcagcaatg tgttttttaa
     1561 cgtgttgttt atgatataaa acttgtacag gtagttgact actataatga aaaactcgtt
     1621 ttaaagatgc ttcatacaat actatttgta attgctgtcc accagtttgt tgccacaaac
     1681 ttaataataa cgtctctaag aaacggtgtg ctgtttcatg cgttttatga acacatttta
     1741 taataatcgg ctgttgataa ttaattggcc ataacaatgg ctgaccggta tttagtttgc
     1801 ccaaacgcca aaataatggt aattgttgtt tctcaatagg ctgatctaag gccaaacttt
     1861 gtttggtaat cacaacgcct cctatactag ggactattga tgtttggcag ataagcaata
     1921 atcaaaacgc caacagcccc tattattatt taagaaatac tggcttttat tgccgtaact
     1981 gctttaatcg ttcagcaatc ttcccctcca aaccacgctc ggttggccag taaaaaagtt
     2041 tattttttaa tttatcaggt aaaaattgct gaccaacagc atgtccattt tcatcatgat
     2101 cataacgata ac
//
LOCUS       DAOQMB010000404         2111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_34965_length_2111_cov_4.685798, whole genome shotgun sequence.
ACCESSION   DAOQMB010000404 DAOQMB010000000
VERSION     DAOQMB010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34965_length_2111_cov_4.685798"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1311)
                     /locus_tag="PLY05_10460"
     CDS             complement(<1..1311)
                     /locus_tag="PLY05_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03760.1"
                     /translation="MKLYEALKKAKSEEDVKDIYIKALGLKSFTKGLIDIQTKEMWFE
                     AKDTGKHSSYAMFTQLLHYVQQSLNKGEAVPPFLAVIDTEKAAIMKSSDVIPFLEKKT
                     IKWGKSASQYTREALEAVSAYIGTYFVSFKLATHEEEFISTVKAAIKSGDIIRTQITP
                     DNLKQVFDKWVNLIGKEINGVAETDYALLFFADIMNDGKISTHKDLPAKLVFMDDKPA
                     FMLNGKVYELGNKEGYRRFWAIYHRPPKEEYRNYLLERRDTLIPLEERSFKGAYYTPL
                     AVVDKAYDLLAETLGNNWQKNYIVWDMCCGVGNLEVKHSNPRNVFMSTLDQADIDVMR
                     ASKICVAATRFQYDYLNDDITDEGKIDYSLTQKVPAALQQAIKDGKKILVLINPPYAE
                     AANSDNTTDNMGAENKKGVSKTKVAASMGDYGYATRELFIQFLIR"
     gene            complement(1344..1730)
                     /locus_tag="PLY05_10465"
     CDS             complement(1344..1730)
                     /locus_tag="PLY05_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014980410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03761.1"
                     /translation="MRRILLDSCVWLWWLDDNPALGKQAKALINDARNQVFISAATVW
                     EIAIKRQKGNLQIDGDLQNLVLEDGFIALNMDLFHAQQAAMLPPIHQDPFDRMLITQA
                     QAEGLELMTADTVIPKYGVRIILARV"
     gene            complement(1727..1972)
                     /locus_tag="PLY05_10470"
     CDS             complement(1727..1972)
                     /locus_tag="PLY05_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015068187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC03762.1"
                     /translation="MSIIQVNMHEAKSQLSYLSERVLAGDKVIIAKAGKPCVELLPHV
                     QTQPVRQAGRLKGKIVIPDDFDSTANDIIDSFYGSQL"
BASE COUNT          624 a          463 c          321 g          703 t
ORIGIN      
        1 cctaattaaa aattgaataa ataactcacg agtggcataa ccataatcgc ccatacttgc
       61 cgccactttg gttttagata cgcctttttt attttctgcc cccatattat cagtagtgtt
      121 gtcagagttc gccgcctccg catatggcgg atttatcaac accaatattt ttttgccgtc
      181 tttaatggct tgttgcaagg cggcaggcac tttttgagtg agtgaataat caatcttgcc
      241 ttcatccgta atatcatcat ttaaataatc atactgaaaa cgtgtcgctg ccacacaaat
      301 tttactggct ctcatcacat caatatctgc ttggtcgagc gtactcataa acacattacg
      361 cggattactg tgtttaacct ccaaattgcc cacaccacaa cacatatccc acacaatata
      421 atttttttgc caattattac ccaacgtctc ggctaacaaa tcataagcct tatctaccac
      481 cgctaaaggt gtgtaatacg cgcccttaaa actacgttct tctaaaggaa ttaaggtgtc
      541 tctgcgctct aacaaataat tacgatattc ttcttttggc ggacggtgat aaattgccca
      601 aaatcggcga tagccttctt tattacctaa ctcatatact ttgccattga gcataaatgc
      661 aggtttatca tccataaata ccagtttggc aggtaggtct ttatgggtac taattttgcc
      721 atcattcata atatcggcaa aaaacagtaa ggcataatcg gtttcggcca cgccgttaat
      781 ttctttacca attaaattaa cccatttatc aaatacttgt tttaagttat cgggcgtaat
      841 ttgggtgcga ataatatcgc ccgattttat ggcagcttta acggtggata taaattcttc
      901 ttcatgcgtt gctaatttaa acgatacaaa gtatgttcca atataagcag aaaccgcttc
      961 taacgcctct cgggtatatt ggctggcaga ctttccccat ttaatggttt tcttttctaa
     1021 aaagggaatc acatcactgc ttttcataat cgcagctttt tcggtatcaa tcaccgcaag
     1081 aaatggtgga acagcttcgc ctttattgag tgattgttgt acataatgca acaattgcgt
     1141 aaacatcgca taactgctgt gttttcctgt atctttggcc tcaaaccaca tttctttggt
     1201 ttgaatatct atcaaccctt tagtaaaact ctttaatcct aaagctttaa tataaatatc
     1261 ttttacatct tcttcgcttt ttgctttttt aagtgcttca tataatttca taaatactcc
     1321 gttatatctt ttaaatcaat gcgttaaaca cgcgccaaaa ttattcttac gccatattta
     1381 ggaatcactg tatcagccgt catcaactct aaaccttcgg cttgtgcttg agtaatcaac
     1441 attctatcaa aagggtcttg atgaatcggt ggcaacatgg ccgcctgttg agcatggaat
     1501 aagtccatat ttagggcaat aaaaccatct tctaacacta aattttgtaa gtcaccatca
     1561 atttgcaaat ttcccttttg ccttttaatc gcaatttccc atacggttgc tgcactaata
     1621 aacacttgat tacgagcatc attaattagt gcttttgctt gtttacctaa cgctggatta
     1681 tcatcaagcc accacaacca tacacaacta tcaagcaaaa ttcgtctcat agctgactgc
     1741 cataaaaact atcaataatg tcatttgctg ttgaatcaaa atcatcaggt atgactattt
     1801 taccttttaa ccgccctgct tgacggactg gttgtgtttg aacatgaggt aataattcaa
     1861 cacatggttt tcctgcttta gcaattatca ctttatcacc tgctaataca cgctcagaca
     1921 agtatgacaa ctgacttttt gcctcgtgca tattgacttg aataatagac atgattttta
     1981 ccttagctaa acttagtcta gttttaatgt agtttaaata gattttttta gcaaccaaaa
     2041 aagtcactgt gataaccatt gcccaacccc caaaaagtat ctacaatgaa tatacaaacc
     2101 aattaggagt c
//
LOCUS       DAOQMB010000405         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35325_length_2100_cov_13.204890, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000405 DAOQMB010000000
VERSION     DAOQMB010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35325_length_2100_cov_13.204890"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2100
                     /gene="ppc"
                     /locus_tag="PLY05_10475"
     CDS             <1..>2100
                     /gene="ppc"
                     /locus_tag="PLY05_10475"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HNC03763.1"
                     /translation="GAPAQAGSVRELLPRLLSKGLNPEQIWQAIQQLDIELVLTAHPT
                     EISRRTLIQKYDNIAECLQALDYIHLTPIERQHQLDKLKRHIIAAWRTDEIRRQRPTP
                     VDEAKWGFATIEQTLWYSIPQFLREFDAVIFEHTGHHLPLTQAPIRFASWMGGDRDGN
                     PNVTHAVTQEVLLLSRWQAADLYWRDIDALRGELSMQDCSPSLRAVVGDNCREPYREL
                     LRTVRDRLAATRDWLTARLRGEIYDDSQIYQHSEQLLQPLLLCYESLISCNMADIAHD
                     ELANIIRRVACFGLQLLKLDIRQESTRHKDALNAITDYLQLGDYQQWNETQRREFLLQ
                     ELNNPRPLLPRHLKTQADSLINQAPVQEVLATFKMLAQQPSESLGAYVISMAKQASDV
                     LAVMLLQKEAEIAQPMRVVPLFETLADLNNAAKCLDELLSIDWYKQAINGHQEVMIGY
                     SDSAKDAGFLTANWAQYRAQEELTAVAKQHGIKLTLFHGRGGSISRGGAPTHQALLSQ
                     PPESVQGKIRVTEQGEMIRFKFGMQGIALRNLELYAAATLEATLLPPPAAKAEWRELM
                     TRMTQTSLAVYKETVHGEPEFIRYLRTVTPELELQMLPLGSRPARRHMNGGIESLRAI
                     PWVFAWTQVRLMLPAWLGTGRALSHAMRDGYTPVIKDMREHWPYFQTLLDMLEMVLAK
                     ADSAIAAHYEQQLTDDPA"
BASE COUNT          578 a          480 c          466 g          576 t
ORIGIN      
        1 ggtgcgcccg ctcaagcagg ttcagtacgc gaattattac cgcgcttatt aagcaaaggg
       61 ctaaatcctg aacaaatttg gcaagcgatt caacaactgg atattgagct agttttaacc
      121 gctcacccca ccgaaattag tcgccgtacc ctaattcaaa aatacgataa tattgctgag
      181 tgcttacaag cccttgatta tattcactta acacccatcg agcgccaaca ccaactcgac
      241 aaactcaaac gccatattat tgccgcatgg cgcaccgatg aaattcgccg tcaacggcca
      301 acccctgttg atgaagcaaa atggggcttt gcgactatcg aacaaacttt atggtatagc
      361 attccgcaat ttttacgtga atttgatgcg gtaatttttg agcatacagg ccatcattta
      421 cctttaactc aagcaccgat tcgttttgcc tcatggatgg gtggcgaccg tgatggtaac
      481 cctaatgtca ctcatgcggt aactcaagaa gtattattgt tatcacgctg gcaagctgcc
      541 gacctttatt ggcgtgatat tgatgcttta cgtggcgaat tatccatgca agattgtagc
      601 ccttccctac gtgctgtggt gggcgataat tgccgtgaac cgtatcggga attattaaga
      661 acagtgcgtg accgcttggc ggcaactcgt gattggctca cagcacgttt acgtggtgag
      721 atttatgatg atagccaaat ttatcaacac agcgaacaac ttttacaacc cttattattg
      781 tgttatgaat cattaattag ctgcaatatg gccgacattg cccatgatga acttgctaac
      841 attattcgcc gtgtcgcttg ttttggtttg caattactca agctcgatat tcgtcaagaa
      901 tccacccgac acaaagatgc gctcaatgcc attaccgatt atttacaact gggtgattat
      961 caacaatgga atgaaacaca acgtcgtgaa tttttactac aagaactcaa taatcctcgg
     1021 cctttattac ctcgtcactt aaaaacacag gcagatagct taattaacca agcccctgtg
     1081 caagaagttt tggcgacgtt taagatgttg gcacaacaac cttcggagtc attgggtgcg
     1141 tatgtgattt cgatggccaa acaagctagt gatgttttag cggtgatgtt attacaaaaa
     1201 gaagccgaaa tcgcccagcc aatgcgtgtt gtaccgttat ttgaaacttt ggctgacctt
     1261 aataatgccg ccaagtgttt agatgaatta ttgagcattg attggtataa acaagcgatt
     1321 aacggccatc aagaagtgat gattggttac tctgactccg ccaaagatgc aggcttttta
     1381 accgccaatt gggcacaata tcgtgcccaa gaagaactga cggcggttgc caaacaacat
     1441 ggcattaaac tgacgttatt tcatggtcgt ggtggctcta ttagtcgagg tggtgcgccc
     1501 actcatcaag cgttgttatc tcaacctcct gaatcagtac aaggaaaaat tcgcgtcacc
     1561 gaacaaggtg aaatgattcg ctttaaattt gggatgcaag gcatcgcctt acgcaatttg
     1621 gagttatatg cagcagcgac tttagaagca acattactgc ctcctcctgc ggccaaagcg
     1681 gaatggcgcg aattgatgac gcgcatgacg caaacttctt tagccgttta taaagaaact
     1741 gttcatggcg agcctgagtt tattcgttat ttacgcaccg tcacgcctga gctagaacta
     1801 caaatgttgc cgcttggctc tcgacctgct cgccgccaca tgaatggtgg gatagagtcg
     1861 ttacgtgcga ttccttgggt gtttgcatgg acacaagtgc gcttaatgtt gcctgcatgg
     1921 ttaggcacag gtcgggcatt gagtcatgcc atgcgtgatg gttacacgcc tgtcattaaa
     1981 gacatgcgtg agcattggcc atactttcaa accttgttag atatgctcga aatggtatta
     2041 gccaaagccg attcagcgat tgccgcacat tacgaacaac aactgacaga tgatcctgct
//
LOCUS       DAOQMB010000406         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35372_length_2099_cov_3.055773, whole genome shotgun sequence.
ACCESSION   DAOQMB010000406 DAOQMB010000000
VERSION     DAOQMB010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35372_length_2099_cov_3.055773"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2099
                     /locus_tag="PLY05_10480"
     CDS             <1..>2099
                     /locus_tag="PLY05_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03764.1"
                     /translation="MYFDGVSLTKTGATHSTSASHTGNLHTTINLDIGSSGASTGVAL
                     QSSAYFGTLKADSLKICDSTGATCGDSYGSFAVQSQEDIVFNLTTQNGLFNKNALMNV
                     DLGLKNLNFYLTQQAAGAINNQFIVRNFNFNFTGKGYIYVGDSTETNPSYQNALVLET
                     RDAANYIDLNKVEDVDYAGKFNAGLNIDMVYKGNTGTTLSTKDPMNPVKGVLRLGASG
                     RATNASLVVKGVDASTILGAAYAVDGVTAGTGTGGSLAGSQGLYMRVRADFTKKVWDS
                     VTNTYNNDDAGIVGGFADNGEGSTLELGHGGTNAYGVEFSNLTPLLIRKDLSLAGRPL
                     NTDRAYFDTGDVYFNLINSKKVQLPVNDTLNNSRLTIGSTTGNITTDADYSHLVHNAA
                     ANPNALAIAVRGFNFNAVARTTRFIASSDVTGADIPTNAAQNWGLGLPFYNLNANMIT
                     YGTQAPDGSERLGFALGLSTEGVNKTALPDGTPIGARTTSIMVIDGASNALDGGNPTN
                     YYIGLRNIDMLITAYGTLGLENNKINLTMPKLTIAGAMELAGGYLPGSRYSSNFGSGA
                     GQCTAANEVACYVSTDSFTKKDDVFLGVKLKLDGSMNLDIVPGVDTLSGNRLSFEGNY
                     DLKGNVTESGVQYTTSTIQFVDPVDGSIVGFDNITGNIGFNNQIKINKDTIAFSYAFT
                     FNPNQVQDKVFRVRDIN"
BASE COUNT          637 a          321 c          472 g          669 t
ORIGIN      
        1 atgtactttg atggagtgag tctaactaaa acaggggcaa cacatagtac aagtgcttct
       61 catacaggca atcttcatac cactattaat ttagatattg gctcaagtgg tgcttctaca
      121 ggagtcgctt tgcaatctag tgcttatttt ggcacgctta aagccgatag tttaaaaatt
      181 tgtgacagta ctggtgcaac ttgtggtgat agctatggca gttttgcggt tcaatcacaa
      241 gaagatatag tttttaactt aacaacacaa aatggcttgt ttaataaaaa tgcacttatg
      301 aatgtggatt tggggcttaa aaaccttaat ttttatttaa ctcagcaggc agctggagcg
      361 attaataacc agtttatagt aagaaatttt aattttaact ttacagggaa aggttacatt
      421 tatgtgggtg atagcactga aacaaatcca agttatcaaa acgctttagt tttagaaaca
      481 cgtgacgcgg ctaattacat agatttaaat aaagttgaag atgtcgatta tgcaggaaaa
      541 tttaatgctg gcttaaatat agacatggtt tacaaaggaa atacaggcac aactttatct
      601 acaaaagacc caatgaatcc agttaaagga gtgttacgtt taggtgcttc tggtcgtgct
      661 actaatgcaa gtttagttgt taaaggagtg gatgcaagca ctattttggg agcggcttat
      721 gctgttgatg gtgtaacggc agggacaggg acgggtggtt ctttggcggg tagtcagggt
      781 ttgtatatgc gtgttagagc tgattttacc aaaaaggtgt gggactctgt taccaatact
      841 tataataatg atgatgctgg tatagtcggg ggatttgctg ataatggcga aggcagtaca
      901 ttagagctag ggcatggtgg gactaatgct tatggtgttg agtttagtaa tttgacccct
      961 ttgttaatta gaaaagattt gtctctcgct ggtaggccgc ttaatacaga tagagcatat
     1021 tttgatacag gcgatgtgta ttttaactta atcaactcca aaaaagtgca gttgcctgtt
     1081 aatgacacat taaataattc gcgcttaacc attggtagta ccacaggcaa tattacaacc
     1141 gatgccgact attcccattt agtgcataat gctgctgcta atcccaacgc tttagcaatt
     1201 gctgttagag gatttaattt taatgcagta gcacgaacaa ctcgctttat agcaagtagt
     1261 gatgtgacag gggcagatat ccctactaac gcagcgcaaa attggggatt aggtttgccc
     1321 ttttataatc tcaatgccaa tatgattact tatggaactc aagcacctga tggttcggag
     1381 cgtttaggat ttgctttggg attaagcaca gaaggtgtca ataaaaccgc tctgcctgat
     1441 ggtacaccaa taggtgcacg tacgacgtct attatggtga ttgatggtgc atcaaatgcg
     1501 ttagatggtg gtaatccgac gaactattat attggtttac gcaacattga tatgttaatt
     1561 acggcttatg gcacattggg tttagaaaat aataaaatta atttaacgat gcctaaactt
     1621 actatagcag gtgccatgga gttagcaggt ggttatttac caggctctcg ttacagctct
     1681 aactttggta gtggtgcggg gcaatgtacg gctgctaatg aagttgcttg ttatgtttct
     1741 acagatagtt ttactaaaaa agatgatgtg tttttaggag ttaaactgaa gctagatggc
     1801 agtatgaact tagacattgt gcctggtgtt gatacactat caggcaatcg tttaagtttt
     1861 gagggtaatt atgacttaaa aggtaacgtc acagaaagtg gtgttcaata tacaacaagt
     1921 accattcaat ttgttgatcc tgttgatggc tctattgttg gttttgataa tattacaggt
     1981 aatattggtt ttaataacca aattaaaatc aataaagata caattgcttt tagttatgct
     2041 tttacgttta atccaaatca agtgcaagac aaagtattta gagttcggga tattaactt
//
LOCUS       DAOQMB010000407         2097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35411_length_2097_cov_13.713516, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000407 DAOQMB010000000
VERSION     DAOQMB010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35411_length_2097_cov_13.713516"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..440
                     /locus_tag="PLY05_10485"
     CDS             96..440
                     /locus_tag="PLY05_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNC03765.1"
                     /translation="MSQKNPCLTCGACCGNFRVSFYWAETTLGQENGVPIELTENINS
                     FYNCMKGTNQTNPRCIALIGTIGETVSCSIYPQRSSTCREFNIIDEQGNIDDRCTKAR
                     AKYGLLPIVNLE"
     gene            complement(475..1263)
                     /locus_tag="PLY05_10490"
     CDS             complement(475..1263)
                     /locus_tag="PLY05_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HNC03766.1"
                     /translation="MGFMNGKRVLIVGVASKLSIAYGIAAAMHREGAQLAFTYQNEKL
                     KARVEEFAANFGSNLVFPCDVSNDEEINQLFANLGQQWDSLDSLVHSVAYAPADQLEG
                     DFTDVTTREGFRMAHDISSYSLTALTKAAKPLLVKAQGNVITLTYQGSERVLPNYNVM
                     GLAKASLEANVRYLASSLGPLGVRVNGISAGPIRTLAASGIKNFRKMLAYNEAATPLR
                     RNVTIEEVGNAAAFLCSSLASGISGEILYVDGGFNTTAMPAGEI"
     gene            complement(1277..1996)
                     /locus_tag="PLY05_10495"
     CDS             complement(1277..1996)
                     /locus_tag="PLY05_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03767.1"
                     /translation="MKLIIALLFLVLQSMTYAQECVVLLHGLGRSTWSMRQMQSTLEK
                     QKYLVINQNYLSREKSIPELAQIVGETLQRCEKLQPTAIHFVTHSLGGILVRAYFHQH
                     PTHPLVKRVVMLGAPNRGSEVVDRYKNSWWFKWMTGPAGQQLGTDTTGLLHQLKPLPF
                     EVGIIAGNSSSDPWFAALFSGANDGKVSVESTKLTEMKDFIEVENGHTFMANSRNVIN
                     QVSAFLATGSFIKKQHLATRK"
BASE COUNT          657 a          414 c          393 g          633 t
ORIGIN      
        1 cgtttctaat tactaaaagc tttatttgat tataacgccc caattggggc gttatttatt
       61 ttgtaatgtt aacctaattt gtttttagaa ttgttatgag ccaaaaaaat ccttgtttaa
      121 catgtggagc atgttgtgga aattttagag tatcatttta ttgggcagaa accactttag
      181 ggcaagaaaa tggtgtgcct attgaactta cggaaaatat taatagtttt tacaattgta
      241 tgaagggaac aaatcaaact aatccgcgtt gtattgcact gataggaact attggtgaga
      301 cagtttcttg ttcaatttac cctcaacgct caagcacttg ccgagaattt aatattattg
      361 atgagcaggg caatattgat gatcgttgta cgaaagcacg tgctaaatat gggttattgc
      421 ctattgtgaa tttggaatga cttacgttgt tgcaacgtaa gtcatcatac acggttaaat
      481 ctcgccagca ggcattgcgg tagtattaaa accaccatca acatataaaa tttcaccact
      541 aatgccagag gctaatgaag agcataaaaa tgcagccgca ttgcctactt cttcaatagt
      601 gacattacgg cgtaaaggtg tagcggcttc attataagcg agcatttttc taaagttttt
      661 aatgccacta gcggctaagg tacgaatggg gcctgcagag atgccattca cgcgcacacc
      721 caaagggcct aagctggaag ctaaataacg cacattggct tctaaagagg ctttggctaa
      781 acccatgacg ttataattgg gcaaaacacg ctctgaacct tgataggtta aagtaattac
      841 attgccttga gctttaacca gtagtggctt tgcagctttt gttagcgccg ttaagctata
      901 actactaata tcatgcgcca ttctaaagcc ttcacgggtg gtgacatcag taaaatcacc
      961 ttctaattga tcggcaggag cataagcaac cgaatgcacc aaactatcta agctatccca
     1021 ttgttgacca agattggcaa ataattgatt aatttcctca tcattactca catcacaagg
     1081 aaacactaaa ttagagccaa aattagcagc aaattcttca acgcgtgctt ttagtttttc
     1141 attttgatag gtaaaggcta attgtgcgcc ttcacgatgc atggctgcgg caataccata
     1201 agcaatagat aatttgctag caacaccgac aattaacaca cgtttaccgt tcataaaacc
     1261 cataattagc tccaatttat ttgcgagtgg caagatgctg ttttttgata aaagagcctg
     1321 ttgccaaaaa agcactgact tgattaatca cgttgcgcga gtttgccata aaagtatgac
     1381 cgttctctac ctctataaaa tctttcattt cggtcagttt ggtactttcg acagaaactt
     1441 taccatcatt tgcaccacta aataacgctg caaaccacgg atcagagcta gagttgcctg
     1501 caataatgcc tacttcaaaa ggaagcggtt ttaattgatg gagtaaacct gttgtatctg
     1561 tacctaattg ttgtcctgct ggccctgtca tccatttaaa ccaccagcta tttttataac
     1621 gatcaacgac ttcgctgcca cgattgggcg cacctaacat caccacacgt ttaactaaag
     1681 gatgagtagg gtgttgatga aaataagctc tcaccaaaat gccacctaga gaatgcgtga
     1741 caaaatgaat agccgttggt tggagttttt cacaacgttg aagtgtttca ccaacaattt
     1801 gtgcaagttc aggaatagat ttttctcgag ataaataatt ttgattaatc actaaatatt
     1861 tttgtttttc taaggtgctt tgcatttggc gcattgacca cgtagagcga cctaaaccat
     1921 gaagtaatac cacacattct tgagcataag tcatagactg taataccaaa aacaataatg
     1981 caataatcag tttcatcctt taaatccttg atgccatatc aaaaaaacag ttccttattt
     2041 taacgtaatt agctgcattt aagcactatt ggcaaaagct gcttgcaata attgttg
//
LOCUS       DAOQMB010000408         2096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35439_length_2096_cov_7.886330, whole genome shotgun sequence.
ACCESSION   DAOQMB010000408 DAOQMB010000000
VERSION     DAOQMB010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35439_length_2096_cov_7.886330"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..682)
                     /locus_tag="PLY05_10500"
     CDS             complement(107..682)
                     /locus_tag="PLY05_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019458110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC03768.1"
                     /translation="MPISLSKGGNVNLSKEAPSMTKMIVGLGWDVRATDGAAFDLDAV
                     AFVLGANGKVRNDNDFIFFNNKQNAEGSVIHGGDNRTGDGDGDDETIIVDLSKMPADA
                     DKVAVCVVIYDAESRRQNFGQVSRAYVRVVNDAGQQEIARYDLSEDGSTEAAMIFGEV
                     YRHNGEWKFKAVGQGFKGGLGPLAASYGVNV"
     gene            complement(785..1363)
                     /locus_tag="PLY05_10505"
     CDS             complement(785..1363)
                     /locus_tag="PLY05_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC03769.1"
                     /translation="MALSLQKGGNLSLSKAAPNLTHILIGLGWDVRTTDGAAFDLDAS
                     LFMLGENGKVRSDADFIFYNQAKSSCGSVEHTGDNRTGAGDGDDESIKVNLTGVPANV
                     SRLVVTVTIHEADSRRQSFGQVGGAFMRIVNAADSSEIARFDLSEDYSTETAMIFGEV
                     YRHGGEWKFKAVGQGYSGGLYAMCQQFGVNIG"
     gene            1672..1944
                     /locus_tag="PLY05_10510"
     CDS             1672..1944
                     /locus_tag="PLY05_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03770.1"
                     /translation="MLAFGQTVERLLKTYDEDEQKEFFLKGIAADIANTHKATYNIEI
                     SEIGFWLLLLKILFDTAKQHSVVIFDQEAWIACVSPNTILPELAEL"
BASE COUNT          644 a          475 c          360 g          617 t
ORIGIN      
        1 catctcttta ttttacagaa tccctcaaat catcgctatt tgtgcaacaa agccgctcct
       61 aaataaaagg cgagaaaatc tcgcctttat cacgacctct tagtgtttag acattcacac
      121 cataactggc ggctaatggg cctaaaccac ctttaaaacc ttgaccaacg gctttaaatt
      181 tccattcacc attatgacga tatacttcac caaaaatcat cgcggcttcg gtagaaccgt
      241 cttcggataa atcataacgg gcaatttctt gttgaccagc atcgttaacg acacgcacat
      301 aagcacgaga cacttgacca aagttttgac gacgactttc agcatcataa atcaccacac
      361 acaccgcaac tttgtcggca tcggcaggca ttttgcttaa atcaacaata atagtttcat
      421 cgtcaccatc accatcacct gtacggttat caccaccatg aatcaccgaa ccttcggcat
      481 tttgtttgtt gttaaaaaag ataaagtcgt tatcgttacg cactttacca tttgcgccta
      541 acacaaaagc cactgcatct aagtcgaatg ctgcaccatc agtggcacgc acatcccaac
      601 ccaaaccgac aatcattttg gtcatgcttg gggcttcttt acttaaatta acgtttccgc
      661 cttttgataa actaattggc attttttgct cctacataca ttaaacaatc aataatagat
      721 actactaact aagtttaccc ttcgactccg ctcagggtac ttggtggctg agcgaagtcg
      781 aatcttaacc aatattcaca ccaaattgtt ggcacatcgc atataaacca ccgctataac
      841 cttggccaac ggctttaaac ttccactcac cgccatgacg atatacttca ccaaaaatca
      901 tggctgtttc ggttgaataa tcttcgctta aatcaaaacg ggcaatttcg ctgctgtccg
      961 ccgcattgac aatacgcata aatgcaccgc caacttgacc aaaactttgt cgacgactgt
     1021 ctgcctcgtg aattgttacg gtaacaacca aacgcgacac attagcaggt acccctgtga
     1081 ggttaacttt gatcgactca tcgtcaccat cacctgcacc tgtacgatta tcccctgtat
     1141 gttccaccga accacaggac gatttggctt gattataaaa aataaaatcg gcatcagaac
     1201 ggactttacc gttttcgcct aacataaata aacttgcatc taagtcaaat gctgcaccat
     1261 cagtggtacg cacatcccaa cctaaaccaa ttaaaatgtg ggtcaaatta ggggctgctt
     1321 tacttaaact taaattgcca cctttttgta aacttaaggc catgtctttt tctccttgta
     1381 accgtgttta agatattgag cgttttactg ttttttcaaa gttgaatgta tagtaaattt
     1441 gttacaatag cttctttctt tataaaccgc caattaattt cttagagtgt ttaggcactg
     1501 ttgacgtttt gtgtgtgagc agtgttgctg ataaaaataa aaaggacaaa taatggctaa
     1561 tcgtcacgat gtatatattt gggatgcaga tgtttatggc aagcctcaac actttgaaga
     1621 ggcgatggat atcgcctagc caaacaacgc attgaaacac aaattgctaa tatgctggct
     1681 tttggtcaaa ccgtagaaag attactcaaa acttatgatg aagatgaaca aaaagagttc
     1741 tttttaaagg gaatagcggc cgatattgcc aatactcata aagccactta caacatagaa
     1801 atttctgaga ttggattttg gctgttatta ttaaaaattt tatttgatac tgccaaacaa
     1861 cattccgtgg ttatttttga ccaagaagca tggattgcgt gtgtgtcacc caatacgatt
     1921 ttacctgagt tggcagagct ataatagcaa agaaccgttg aaaaactcac aaattttcat
     1981 aatatttagt gggaaaagga cgaatgatga atattttttt caataaaaaa cccttctagt
     2041 gtttgctaga agggtttttg tatttggagt ctggcgatga cctactctca catggc
//
LOCUS       DAOQMB010000409         2095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35462_length_2095_cov_17.973039, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000409 DAOQMB010000000
VERSION     DAOQMB010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35462_length_2095_cov_17.973039"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="PLY05_10515"
     CDS             complement(<1..1433)
                     /locus_tag="PLY05_10515"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HNC03771.1"
                     /translation="MHAACYYTISTQQPSRHFFQVSLLIRQPEPTGQIVWLPNWILGS
                     YMIRDFARHISSVKAKTANGLLLNVKKLAKNKWQVQAHDGDIILEYDVYAWDLSVRGA
                     HLDQTHGYFNGTCLLLAVQGKENALHQLNLLTPQGLSWRVATTLAAVNIDKNGFGLYQ
                     AANYLDLVDHPVEMADFTELSFTSGQTPHKMVITGKHRADTQRLADDLQKICQAAINF
                     WGDEQPPFNQYLFQVMAVGNGYGGLEHRSSTSLLCSRDDLPLVTEPKQKISEKYKTFL
                     GLCSHEYFHSWLVKRIQPDVLVSPDLNQENYTELLWVFEGFTSYYDDLLLARAGIIST
                     QEYLDLLAQTITRHIKTLGRFKQTVTESSFDAWTKYYKPDENTPNSVVSYYVKGALVA
                     LCLDLKLRQLSQQKISLDMVMRELWLNYGKVGKGVNNNTVQEIAQRLIHQDLAEFFNQ
                     CLYSTEELPFVMLLSEQGIQLSYKYDNG"
     gene            1470..>2095
                     /locus_tag="PLY05_10520"
     CDS             1470..>2095
                     /locus_tag="PLY05_10520"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03772.1"
                     /translation="MNNPHLSTEATLLYLPTPDGHKIPIRHWPVSQPRAVLHFVHGMA
                     EHSGNYDDVAAFFNQHHFAVLAHDHRCHGLSTTQLGNVDKIQNWQGVLEDMPLIHNWI
                     KATYQQTPVIIVGHSMGSFVSQHFVQHHPHAVQALALAGSDFLPTWFTGAASRLAKIE
                     TCRQGANGRSDLIHGLAFGKFGKAIKNARTEFDWLSRNPAWVDKYIADP"
BASE COUNT          650 a          420 c          357 g          668 t
ORIGIN      
        1 ccattatcgt atttataact taactgtatg ccttgttctg acaataacat cacaaaaggc
       61 aattcttctg tagaatataa acactgatta aaaaactcgg ctaaatcttg atgaattaaa
      121 cgctgagcaa tttcttgaac agtattatta ttcactcctt taccaacttt gccataattt
      181 aaccataatt cacgcattac catatctaaa ctaatttttt gctgacttaa ttgccgtagt
      241 tttaaatcta aacacaaggc aactaatgca ccttttacat aataactgac cacactattt
      301 ggtgtatttt catctggttt ataatatttt gtccacgcat caaaactaga ctcggtgacg
      361 gtttgtttaa atcgacctaa agttttaata tgacgagtaa tggtttgtgc caataaatct
      421 aaatattctt gagtagagat aatacctgca cgtgctaata ataaatcatc ataataagag
      481 gtaaagcctt caaataccca taataattcg gtgtaattct cttgatttaa atcaggactt
      541 actaaaacat caggttgaat acgcttaacc aaccaagaat gaaaatattc atgactacat
      601 aaccctaaaa aggttttgta tttttcacta attttttgtt ttggttcagt gactaaaggt
      661 aaatcatcac gcgagcataa taaactggtt gaacttctat gctctaaacc accataacca
      721 ttgcctactg ccatcacttg aaataaatac tgattaaatg gcggctgctc atctccccaa
      781 aaattaatcg cggcttgaca aattttttgc aaatcatccg ctaaacgttg ggtgtctgct
      841 cgatgtttgc ccgtaatcac cattttatgt ggcgtttggc cgctagtaaa acttagctcg
      901 gtaaaatcag ccatttcgac aggatgatcg actaagtcca aataattagc ggcttgataa
      961 agaccaaaac catttttatc aatatttacg gctgccaagg ttgttgccac acgccaagat
     1021 aagccttgag gtgttaacaa gtttaattga tgtaaagcat tttctttgcc ttgaacggct
     1081 aataacaagc aagttccatt aaaataacca tgtgtttgat ctaaatgtgc gccacgtact
     1141 gataaatccc atgcatatac atcatattct aggataatat cgccatcgtg cgcttggact
     1201 tgccatttgt tttttgctaa ttttttgaca tttaataaca agccattggc agtttttgcc
     1261 tttacactgc taatatgtcg cgcaaagtca cgaatcatat agctacccaa aatccagttt
     1321 ggcagccaaa caatttgccc tgtcggctca ggttggcgaa ttaacaacga tacttgaaaa
     1381 aaatgacgac ttggctgttg ggtagaaata gtgtaataac acgctgcgtg cataaatttt
     1441 accgttttat agaataattg gattaaagaa tgaacaaccc gcatcttagt acagaagcga
     1501 ctttattata tttacccaca cctgatggcc ataaaattcc tattcgccat tggcccgttt
     1561 cgcaaccacg tgcagtcctt cattttgttc atggcatggc cgaacatagt ggtaactatg
     1621 atgatgttgc agcttttttt aatcaacatc attttgcagt tttagcacac gatcatcgtt
     1681 gtcatggctt atcaaccaca caattaggta acgttgataa aattcaaaac tggcaaggcg
     1741 tactagaaga tatgccttta attcataatt ggattaaagc aacttatcag caaacgcctg
     1801 tcattattgt tggtcacagc atgggttctt ttgtcagcca acattttgta caacatcacc
     1861 ctcatgcggt gcaagcctta gcattagctg gttcagactt tttacccact tggtttacag
     1921 gtgcagcatc acgtttagca aaaattgaaa cttgtcgtca aggggcaaat ggtcgtagtg
     1981 atttaattca tggcttggct tttggtaaat ttggcaaggc gattaaaaat gcccgtaccg
     2041 agtttgactg gttatcacgc aatccagcat gggttgataa atatattgct gatcc
//
LOCUS       DAOQMB010000410         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35625_length_2090_cov_14.501229, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000410 DAOQMB010000000
VERSION     DAOQMB010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35625_length_2090_cov_14.501229"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLY05_10525"
     CDS             complement(<1..215)
                     /locus_tag="PLY05_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004797814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC03773.1"
                     /translation="MKSDRILIVDDEAAIREMISIALDLAGFDTIEAEDALQAHHKIV
                     DERPSLVLLDWMLPGMTGIELCRRLKR"
     gene            complement(389..1975)
                     /locus_tag="PLY05_10530"
     CDS             complement(389..1975)
                     /locus_tag="PLY05_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNC03774.1"
                     /translation="MNIKDIHKLEQATKKGRTELFRLGLGLIFMVGVMLYASAKGVAG
                     NEGTLVIVIAAAIGAYMAMNIGANDVANNVGPAVGSKALTLFGALVIAAIFEAAGALI
                     AGGEVVGTIRNGIINPNLITDSQTFVWIMMAALLAAALWLNVATAIGAPVSTTHSIVG
                     AIIGSGIAASGMDIVNWGKMTEIAASWVISPVLGGLVAAAMLYFVKRTIIYQSDMVGA
                     ARKIVPWLIAFMGWTFGTYLILKGLNRVWKVSFLDASLIGVGIAVLLFLFTRWRLSQF
                     SPQQDAKQSVNQLFTIPLIFAAALLSFAHGSNDVANAVGPLAAIVDVYSSVDHIVHKE
                     AAIPMWVMVVGALGISLGLALYGPKVIRTIGSEITDLDQTRAYCIAMSATITVIIASQ
                     MGMPVSSTHIAVGGVFGVGFLREYLKYNYDKMVAQIKAHHPEGDQVAIDDFLAKFNKA
                     SIDEKGVLLRELKAQAKRQMDPAHFSKAERNALKKVYRTQLVQRSQLLRIAAAWVITV
                     PATALMAGLIFFIIKGVTAS"
BASE COUNT          674 a          447 c          400 g          569 t
ORIGIN      
        1 tctcgtttta aacgacggca taactcaata cctgtcatac caggtaacat ccagtctaat
       61 aaaacaaggc tagggcgttc atcaacaatt ttatggtggg cttgtaaggc atcttcggct
      121 tcaatggtat caaaacctgc taaatcgagg gcgatgctaa tcatttcacg aatggcggct
      181 tcgtcatcga caattaaaat acggtcactt ttcatggagc ttttcctcgt acttaagacg
      241 actaggctaa taagagtctt gagtcggttg tttgaggcta ttacatgaca aacgtgttgc
      301 agtattatga caaatacaaa tgatgatgaa aataaaaagg ctgttgtttt cacaacagcc
      361 tttagtttaa aaactcaata gatagtgttt agcttgctgt tacgccttta ataataaaga
      421 aaattaatcc agccattaat gctgttgcgg gtacagtaat cacccacgca gcagcaatac
      481 gtaataattg tgagcgttgt actaattggg tacgataaac ttttttgagg gcattgcgtt
      541 ctgctttaga aaaatgggca ggatccattt gtcttttggc ttgcgctttt aactctctta
      601 ataaaacgcc tttttcatca atagaggcct tattaaattt ggctaaaaaa tcatctatag
      661 cgacttggtc accttcggga tgatgggctt tgatttgagc gaccatttta tcgtaattat
      721 attttaaata ttcgcgtaaa aagccaacac caaatacgcc accaacggca atatgggtag
      781 agctgacagg cattcccatt tgactggcaa taatcacggt aattgttgct gacatagcaa
      841 tacaatatgc tctagtttgg tcaaggtcgg taatttcaga gccaattgtt ctaatgactt
      901 tagggccata taaagccaaa cctaaagaaa tacctaatgc accaaccacc atcacccaca
      961 tgggaatagc cgcttcttta tgaacaatat ggtcaacaga actataaacg tcaacaatcg
     1021 ctgctaaagg gcctacggca ttagcaacat cattagagcc atgtgcaaaa cttaataagg
     1081 ccgcagcaaa aatgagtgga atagtaaata attgattaac actttgtttg gcatcttgtt
     1141 ggggactaaa ttgacttagt ctccaccgtg taaataaaaa taataacacc gcgatgccca
     1201 cgccaattaa actcgcgtct aaaaaactga ctttccaaac gcggtttaag cctttgagta
     1261 taagatacgt gccaaatgtc caccccataa aagcaattaa ccacggcaca attttacgcg
     1321 cagcacccac catatcgctt tgataaataa tggttctttt aacaaaatac aacattgccg
     1381 cagcgactaa accacctaat acaggcgaaa tgacccaact ggcagcaatt tcagtcattt
     1441 tgccccaatt aacgatatcc ataccactgg ctgcaatacc actgccaata attgcaccaa
     1501 caatagaatg tgttgtggag acaggtgcac caatagcggt tgccacattt aaccataatg
     1561 ctgctgctaa taaagccgcc atcatgatcc aaacaaaggt ttgtgaatca gtaattaagt
     1621 tagggttaat aatgccatta cggatagtgc cgacaacttc gccccccgca attaatgcac
     1681 ctgctgcttc aaaaatagca gcaatcacta aagcaccaaa taaggttaag gcttttgagc
     1741 caaccgcagg accaacatta ttggcgacat cattagcacc aatgttcatg gccatataag
     1801 caccaatcgc ggcagcgatg acaatcacta gcgtaccttc gttacctgca acacctttgg
     1861 cggaggcata cagcataaca cccaccataa aaattaagcc taagcctaaa cgaaatagct
     1921 cagtgcggcc ttttttggtg gcttgttcca atttatgaat atctttgatg ttcacgatgg
     1981 atttctcaga tgaatttggg aggcggatta tgacagaaat gtgacaatag atggtgtaaa
     2041 gcggcgttat tgaaaaacaa atagtggtgt ttttttaatc gcagacaaga
//
LOCUS       DAOQMB010000411         2088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35694_length_2088_cov_18.195770, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000411 DAOQMB010000000
VERSION     DAOQMB010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35694_length_2088_cov_18.195770"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..740
                     /locus_tag="PLY05_10535"
     CDS             129..740
                     /locus_tag="PLY05_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
                     /protein_id="HNC03775.1"
                     /translation="MEHLSIGETGKLLGVSVSTLRRWEVEKKLLPAYRTIGGHRRYLW
                     QDIQHFCGQFAPELRKTIAYARVSSHDQKDDLERQAQRLEQFCTEQGFDNVTVLKDLG
                     SGLNYHKKQFKQLLRMIALGQVAHLVLTHKDRLLRFGADIIFQLCQIHQTQVTIIEQD
                     NDISLEKSLVADVIELMTVFSARLHGSRSHKNKKAVKETVAIC"
     gene            779..1945
                     /locus_tag="PLY05_10540"
     CDS             779..1945
                     /locus_tag="PLY05_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC03776.1"
                     /translation="MLSTAHQIELKPNNKQANYLARACGVARLAYNWGLANWQEKYQA
                     HQQDNSLPKPNEQLLRKEFNAIKHEKYPFVLEVTKYASQQALKNLGTAFKRFFKGEAS
                     YPQFCKKGHHDSFYIGNDQFKVNDKRIWIPNLGWVKTKESYKHQGAGKINNAVISRQA
                     DRWYVSISVSLPIDKKPARQPVEKQDCVGVDLGISHLATCSDGHKVENPKALSKNLKK
                     LKRLSQALSRKVKGSHNRYNAKLQLSTLHQRIKNIRKDALHKLSYYLTTKFNTVVLED
                     LNVKGMMQNGKLSRAIADVGFYELRRQVEYKAKYYGAKVIFADRFYPSSKLCSVCGFK
                     HDGLTLKDREWDCPHCKTSHERDVNAALNLRQLGLTKIAASSTVTACGEFGSGV"
BASE COUNT          710 a          403 c          421 g          554 t
ORIGIN      
        1 aaaagcgtca tgctatctca tgcgattatt tgaatttatt aacattaaaa agggtaatta
       61 acagcacctt gaaaagctat tcaaacctac ttatttagta tattttttag actaaatgag
      121 tttaaagcat ggaacattta agtattggag aaacaggaaa attattaggc gtatcggtct
      181 caacgcttag aagatgggaa gtagaaaaga agttgttgcc tgcgtatcgc accattggcg
      241 gtcatcggcg ttatttatgg caagatatac agcatttttg tggtcaattt gcccccgaac
      301 tgcgtaaaac catcgcttat gcacgagtat cctcgcatga ccaaaaagac gacttagaac
      361 ggcaagccca acgattggag caattctgca cagaacaagg ttttgataat gtcaccgtat
      421 taaaagactt aggcagtggt ttaaattatc ataaaaaaca gttcaaacaa ctacttagaa
      481 tgatagcctt aggtcaagtt gcgcacctcg ttttaaccca caaagacaga ttattgcgtt
      541 ttggtgcaga tattattttt cagttatgtc aaattcatca aacgcaagtc acgattatag
      601 aacaagacaa cgacatcagt ttagaaaaaa gcttagttgc cgatgtcata gaattaatga
      661 ccgtattttc agcacgatta cacggctcac gctcacacaa aaacaaaaaa gccgttaaag
      721 aaacagtagc tatctgttag aattagtcaa tcattttctg ttgaatgaat cgtttttcat
      781 gctcagcacc gcccatcaga ttgaacttaa acccaacaac aagcaagcca actatttagc
      841 gcgtgcttgt ggtgttgcgc gtttagcgta taactggggc ttagcaaact ggcaagaaaa
      901 atatcaagcc catcaacaag ataacagttt acccaaaccg aatgaacaat tactaagaaa
      961 agaatttaac gccatcaaac acgaaaaata tccctttgtt ttagaagtca ccaaatacgc
     1021 ctcccaacaa gcgttaaaga atttaggcac agcgtttaaa cgttttttta aaggtgaagc
     1081 cagttatccg caattttgta aaaaaggtca tcacgactca ttttacattg gcaatgacca
     1141 atttaaagtc aacgacaaac gcatatggat acccaatctc ggttgggtaa agaccaaaga
     1201 aagctataaa catcaaggag caggcaaaat aaacaatgct gtcatcagcc gacaagctga
     1261 tagatggtat gtgagtattt cggtgagttt gccgatagac aaaaagccag cacgtcagcc
     1321 agtcgaaaaa caagattgtg tgggtgtgga tttaggaatt agccatttag ccacctgttc
     1381 agatgggcat aaagtggaaa atcctaaggc tttatcaaaa aacctaaaga aactcaaacg
     1441 cttaagccaa gcgttaagtc gcaaggtaaa aggctcacac aatcggtaca acgccaaatt
     1501 acaactgtcc acactgcatc aacgcatcaa aaacattcgc aaagatgcct tacacaaact
     1561 gtcttattac ctgacaacaa aatttaacac cgttgtttta gaagacctaa atgtgaaggg
     1621 tatgatgcaa aatggaaagt tatcgagagc catcgctgat gttggctttt acgaattaag
     1681 acgacaggta gaatacaagg cgaagtatta cggtgcaaaa gttatttttg ccgaccgttt
     1741 ttatccaagc tcaaaattgt gttcagtgtg tggttttaag catgacggtt tgacgttaaa
     1801 agaccgtgaa tgggattgtc cacactgtaa gacaagccat gagagagatg ttaatgctgc
     1861 tttgaattta aggcagctcg gtttaacaaa aattgccgcg agttcaacgg tgaccgcttg
     1921 tggagagttt ggctctggcg tttagtggca acactaagcg tgaaaccagc tctgtgaagc
     1981 aagaaacaag cattaaatct attttaggta gatttgagta agtcttgtgg aacggtctgg
     2041 tctcatacgg tctctggttc agctctggta cattacatat acctcact
//
LOCUS       DAOQMB010000412         2085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_35812_length_2085_cov_16.634975, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000412 DAOQMB010000000
VERSION     DAOQMB010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35812_length_2085_cov_16.634975"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="PLY05_10545"
     CDS             complement(<1..1067)
                     /locus_tag="PLY05_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016145890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC03777.1"
                     /translation="MTELNTELEQFRDMVKRFLDKEIKPNYEQWEKDGIIPRDVWFNM
                     GANGLLCVDQPEEYGGVGVSYLYSMVVLEEASRANFASLATGLSVHSDIAAPYVQHIG
                     NEAQRQQWLPKMISGEVIGAIGMTEPGAGSDLQAIRTTAIRNGDHYVLNGSKTFITNG
                     QHADLVILAAKTDPNGGAKGVSLFLVDMNLAGCARGRNLEKMGLHSQDTSELFFDNVK
                     LPLDSLLGEEGKGFAYLMNELPRERLNLAVCAVAASEGLLDATIAYTQERKAFGNPIS
                     KLQNTRFTLATCHTDIAVNRAFINQCIDQYMVGKLDASTAAVAKLSTTDMQCRVADAC
                     LQLFGGYGYMMEYPAARAYVDA"
     gene            complement(1098..1751)
                     /locus_tag="PLY05_10550"
     CDS             complement(1098..1751)
                     /locus_tag="PLY05_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNC03778.1"
                     /translation="MNTVNDIQAGAAIYNKTVLKVYDVWVHGFSNHYLWRCPTTHLVA
                     LYQRYTSNNHLEVGVGTGLFLQRVQFTQLKPRLVLMDLNPNTLEIVSKKLQRIQPAGV
                     INDILAGQQPNLPLFDSIAFNYVWHCLPAEADKEQAFVNLAKFLQPNGVLFGSTLLAK
                     DIQQNLPAKALMKLYQHKGIFGNQYDDLTRLETALQQNFSRYQIQQIGSAALFVAWK"
BASE COUNT          636 a          408 c          422 g          619 t
ORIGIN      
        1 gcatcaacgt aggctcgtgc agcaggatat tccatcatat agccataacc accaaataat
       61 tgtaaacaag catcggcaac acggcattgc atatcggtgg tggataattt tgcgacagca
      121 gctgtggaag catcaagttt gccaaccata tattgatcaa tacattgatt aataaaagca
      181 cgattaacgg caatatcggt atgacaagtc gctaaggtaa agcgagtatt ttgaagttta
      241 ctgataggat taccaaaggc tttgcgttct tgcgtatagg caattgtggc atctaataaa
      301 ccttcagaag ctgcaaccgc acacaccgct aaatttaaac gctcgcgtgg caactcattc
      361 attaagtagg caaatccttt accttcttcg cctaataagc tgtctaaggg taatttgacg
      421 ttatcaaaga ataattcaga cgtgtcttga gagtgtaagc ccattttttc aaggttacga
      481 ccacgcgcac aacctgccaa attcatatcc actaaaaata agctaacgcc ttttgcgcca
      541 ccattagggt cagttttagc cgctaaaatg actaagtcgg catgttggcc attggtaata
      601 aaggttttag agccgtttaa tacataatga tcgccattgc gaatagctgt ggtacgaatc
      661 gcttgtaagt cagagccagc acctggttcg gtcataccaa tcgcaccaat gacttcacca
      721 ctaatcattt tgggtaacca ttgttggcgt tgtgcttcat tgccaatatg ttgcacatac
      781 ggtgcagcaa tgtcggagtg aacggataaa ccagtggcta aagaggcaaa gttagcacga
      841 gaggcttctt ctaaaaccac catagaatat aaataagaga caccaacacc accgtattct
      901 tcgggttggt caacacataa caagccatta gctcccatgt tgaaccacac gtcacgtgga
      961 ataatgccgt ctttttccca ttgttcataa ttaggtttaa tttctttgtc taagaaacgt
     1021 ttgaccatgt cacggaattg ttcgagttct gtattaagtt cagtcatggg gctagcctct
     1081 gaggttggga gtaaaagtta tttccatgca acaaataatg ctgcactacc aatttgttga
     1141 atttgataac gtgaaaaatt ttgttgtaag gccgtttcta gccgtgtgag atcatcatat
     1201 tgattgccaa aaataccttt atgttgataa agcttcatta aggcttttgc aggtaagttt
     1261 tgttgtatgt ctttggccaa tagggtagaa ccaaacaaca caccatttgg ctgtagaaat
     1321 ttggccaaat taacaaaggc ttgctcttta tcggcttcgg caggcaaaca gtgccaaaca
     1381 taattaaatg caatggagtc aaataacggt aagttgggtt gttgacccgc taaaatatcg
     1441 ttgatgacac ccgcaggttg aatgcgttgc agttttttcg agacaatttc tagcgtattc
     1501 ggatttaaat ccatgagtac aagacgcggc tttagttgtg taaattgtac acgttgtaaa
     1561 aatagccccg taccaacacc cacttctaaa tgattgttgc tggtataacg ttgatataac
     1621 gcaactaaat gtgtggttgg acaacgccac aaataatgat tagaaaaacc atgtacccat
     1681 acatcataaa ctttgagtac ggttttgttg tagattgctg cccctgcttg aatgtcattt
     1741 actgtgttca cgtagatatc cgtttgcata aggtattttc tgtacaattg tattgtaaat
     1801 aatttaatta gtatatttgc ttttttgaca aaagtattta ggggctgagc cttttgttac
     1861 aaagacaaag ataggacaaa aactgccaaa aatttaccga aatgcccaaa aaaatggcca
     1921 gacctgtcaa tggtttgtgg ttaaaaatga ttagaataaa aacactaaat atgatgatgt
     1981 gctctgcact ctccataacc accgtttgag gtcataattt atgccagctt ataaggctcc
     2041 attgcgcgat atgcgctttt tgatgaatga agtgtttgat tttcc
//
LOCUS       DAOQMB010000413         2072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_36236_length_2072_cov_4.914229, whole genome shotgun sequence.
ACCESSION   DAOQMB010000413 DAOQMB010000000
VERSION     DAOQMB010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36236_length_2072_cov_4.914229"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="PLY05_10555"
     CDS             complement(<1..205)
                     /locus_tag="PLY05_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03779.1"
                     /translation="MLIVSGAKALSTFKSQRLLADLQAIQPSIVGLISRFVHVVAVNQ
                     ALNDLEQQQLHELLHYGEEFNAEG"
     gene            492..1868
                     /gene="mltF"
                     /locus_tag="PLY05_10560"
     CDS             492..1868
                     /gene="mltF"
                     /locus_tag="PLY05_10560"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HNC03780.1"
                     /translation="MLGKLALPANYFTLKKIVSLKRIILATFLFCGALLCHPSASYHI
                     DKVTDRGTLRVIGIASPTTFLPHGDAGTRGLQYELLRKFAEELKLKLEIEQAPNAEAV
                     VRALRAGKADIGITGLASRDPRLKSVLTSAPYLQEQQQLVGRYNNPITLENSQIAVSN
                     NSIEQLQVQNIEPNAEIISIPNATPDLLLTKLLDGSADYAVLNKAEFEARRAAFPELQ
                     AKETLTDIKLSWALRPKDQHLLKLANYFLQQSSTDGTLQRLTRFYSQGETFNSLGVKT
                     FQRDMQARLPLYQHKFEQQADKHNIDWRLLAAIGYQESKWNANAISPTGVAGLMMLTQ
                     STAKEMGISNRQNPTQSIEAGAAYYQLMTQKFAKTVREPDRTWMALAAYNMGYGNIVK
                     AQKITQQMGNDPSKWLDVSRHLRQLPRAGQALVYVQEVRRYYDALLLTSHKQTWVASI
                     DKRQNAVQ"
     gene            complement(1876..>2072)
                     /locus_tag="PLY05_10565"
     CDS             complement(1876..>2072)
                     /locus_tag="PLY05_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004860823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-specific adenosine deaminase"
                     /protein_id="HNC03781.1"
                     /translation="IHARISRVVFATTEPKAGSLVSARRQLETGYYNHVFSFEGGLLA
                     DEASKQLSDFFRGRREAKKA"
BASE COUNT          623 a          454 c          423 g          572 t
ORIGIN      
        1 aaccctcggc attaaactct tcaccataat gcaataattc atgtaattgt tgttgttcga
       61 ggtcattaag agcttgatta acggccacaa catgcacaaa gcgactaatt aaaccaacaa
      121 tactcggctg tatggcttgc aaatctgcca acaagcgttg acttttgaag gtagataagg
      181 ctttagcacc actaacaatt aacatgacac actcactcat tagagggatg ggagaagaga
      241 taaaactgag ggcgtgtatt gtacccgaat tgtggcaaat ggtggcaatt tcgtcgtttt
      301 tggcctgata tttttagcct acttacttta tcttcttgtc agttatgcac aaaaactcga
      361 cagtctttcg caaacttttt tatcggcaaa tggctacact ttgtcctaca tactcagtat
      421 aaaaaagctg ttgtttgatt tttaagccag aaacgattaa aattcaacca acactctacg
      481 ggaggtcgcg catgttaggc aaattggcct tgccagccaa ctactttacg ctcaaaaaaa
      541 tagtgtcatt aaaacgtatc atattggcaa cttttttatt ttgtggcgca ttattgtgtc
      601 atccttctgc gtcttatcac attgacaaag tgaccgaccg tggcacatta cgtgtgattg
      661 gtattgctag tcctacgact tttttacctc atggcgatgc tggaacacgc ggccttcaat
      721 atgaattact gcgtaaattt gccgaagaac tcaagctcaa gctagaaatt gaacaagccc
      781 ctaatgccga agctgtggtg cgcgccttgc gtgctggcaa agccgatatt gggattacag
      841 gtttggcaag tcgtgacccg cgtctaaaat ctgtattaac gagtgctcct tatttgcaag
      901 agcagcaaca gttagtcggt cgttacaaca accctatcac gctagaaaac tcacaaattg
      961 cggtgagtaa taacagtatt gagcaattac aagttcaaaa catagagcct aatgccgaga
     1021 ttattagcat tcctaacgca acacctgact tgctcttaac gaaattatta gatggttctg
     1081 ccgattatgc agtgcttaat aaggcagagt ttgaggcgcg tcgtgcggct tttcctgagt
     1141 tacaggcaaa agaaactctt acggacatta aactctcatg ggcattacgc cccaaagatc
     1201 aacatttgct caaattagct aattattttt tacaacaaag ttcaaccgat ggcactttac
     1261 aacgccttac tcggttttat agtcaaggcg aaacttttaa tagccttggc gtcaaaactt
     1321 ttcagcgcga tatgcaagct cgtttaccat tgtatcaaca taaatttgag caacaagccg
     1381 acaaacacaa tatcgactgg cgtttattag cagcgattgg ttatcaagaa tccaaatgga
     1441 atgctaatgc tatttcgccc acaggtgttg caggcttaat gatgctaaca caaagtaccg
     1501 ccaaagaaat gggaattagc aatcgccaaa acccaactca aagcattgaa gctggtgccg
     1561 cttattatca gctcatgact caaaaatttg ctaaaacggt acgcgagcct gatagaacat
     1621 ggatggcctt agctgcttat aacatgggtt atggcaatat tgtaaaggca caaaaaatta
     1681 cccaacaaat gggtaatgac ccgagtaagt ggttagacgt aagtcgtcat ttgcggcagt
     1741 taccgcgtgc tggtcaggcg ttggtgtatg tgcaagaagt aagacgttat tatgatgcct
     1801 tgctgttgac cagccataaa caaacttggg tggccagcat agacaaacgg caaaatgcgg
     1861 tgcaataggc taacattagg ctttcttcgc ctctcgcctc cccctaaaaa aatctgacaa
     1921 ctgtttactg gcctcatcgg ccagtaaacc gccctcaaaa ctaaacacat ggttgtaata
     1981 acctgtttct aactgccttc tagcactgac taaagagcct gctttgggtt ctgtggttgc
     2041 aaaaacaact cgactgattc gagcatgaat ca
//
LOCUS       DAOQMB010000414         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_36605_length_2060_cov_17.999002, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000414 DAOQMB010000000
VERSION     DAOQMB010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36605_length_2060_cov_17.999002"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1965)
                     /gene="acs"
                     /locus_tag="PLY05_10570"
     CDS             complement(1..1965)
                     /gene="acs"
                     /locus_tag="PLY05_10570"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HNC03782.1"
                     /translation="MSIESVLQENRQFAPSAEFVAGARVKSLAEYQAMCQQAEADYEG
                     FWAGLANERLTWHKPFTQVLDQSNPPFYKWFADGELNVSYNCLDRHLPDLAHKIAVIF
                     ESDNGTVTKVTYAELHRLVCQFANGLKSLGVTKGDRVVVYMPMSIEAVVAMQACARIG
                     AIHSVVFGGFSAKSLQERIIDAGAKIVITANGGVRGGKIVPLKATTDEALALGGCECV
                     ERVIVYKRVVHEDAPWTIGRDMWWHDLVKGKPETCEPTWVNAEDPLFILYTSGSTGKP
                     KGVQHSSAGYLLGAVNTMLLTFDYRPDDVFWCTADVGWITGHSYVAYAPLAVGATQVI
                     FEGIPTYPDAGRFWQMIERHRVTTFYTAPTAIRSLIKLGGDLPKQYNLSSLRLLGSVG
                     EPINPEAWMWYHEVVGGGRCPIVDTWWQTETGSHMITPLPAAIATKPGSCTLPIPGIM
                     VDIIDEAGEPVEKGKGGFLVIKKPFPSQVRTLWKDPERFKKSYFPEEYGGKYYLAGDS
                     AHKDADGYIWIMGRIDDVLNVSGHRLGTMEIESALVGNTKVAEAAVVGRPDPIKGEAV
                     VAFVVLKGARPSGDEAKALVAELRDWVAKEIGAIAKPDDIRFGDNLPKTRSGKIMRRL
                     LRTIAKGEQITQDISTLENPAILQQLQEAV"
BASE COUNT          577 a          530 c          395 g          558 t
ORIGIN      
        1 tcaaaccgct tcttgtaatt gttgcaaaat cgctggattt tcgagggtag aaatatcttg
       61 ggtgatttgt tcccctttag cgatagttct caacaaacga cgcataattt tacccgaacg
      121 tgttttgggt aaattgtcgc caaaacgaat atcatcaggt ttagcaatcg caccaatttc
      181 tttggcaacc caatcacgca actcagccac taaggctttt gcctcatcac cagacggtct
      241 tgcgcctttt aacaccacaa aagccaccac cgcttcgcct ttaatggggt caggacgtcc
      301 aaccaccgct gcttcggcca ctttggtatt gcctactaaa gcagattcaa tttccatggt
      361 tcccaaacga tgaccagaga cgtttaacac atcgtcaatc cgtcccataa tccaaatata
      421 accatcagca tctttatgcg cagagtcccc tgctaaataa tattttccac catattcttc
      481 aggaaaatac gattttttga agcgttctgg gtctttccac aaggtacgca cttgggaagg
      541 aaacggcttt ttaatcacta aaaagccgcc tttacccttt tcgactggct cgcctgcttc
      601 atcaataata tcaaccataa tccctggaat cggcaaggta catgaacccg gtttagtagc
      661 aatcgccgca ggtaagggcg taatcatgtg gctacctgtt tcggtttgcc accatgtatc
      721 cacaatcgga caacgaccac cacccacaac ttcgtgatac cacatccaag cttcggggtt
      781 aattggctca cccaccgaac ctaataagcg caatgacgac aagttatatt gcttaggtaa
      841 atcaccccct aatttaatta aagagcgaat ggctgtgggt gcggtataaa atgtcgtgac
      901 gcgatgacgt tcaatcattt gccaaaaacg tcctgcatcg ggatacgttg gaatgccttc
      961 aaaaataact tgcgttgccc caacagcaag tggcgcatag gcaacataac tatgacccgt
     1021 aatccaaccc acatcggctg tacaccaaaa cacatcatca ggacgataat caaaggttaa
     1081 taacatggta ttaaccgcac ctaacaaata gcctgcactg ctatgctgga cacctttggg
     1141 tttgcctgtt gaccccgagg tatacaaaat aaataagggg tcttcggcat tcacccatgt
     1201 tggctcgcag gtttcgggtt tgcctttgac aaggtcatgc caccacatat cacgaccaat
     1261 cgtccacggc gcatcttcat gcaccacacg cttataaaca atgactcgct caacacattc
     1321 acaaccaccc aaagctaaag cttcgtctgt ggtggctttt agaggcacaa ttttgccacc
     1381 acgcacgcca ccattggcgg tgatcacaat ttttgcacct gcatcaataa tgcgttcttg
     1441 taaacttttt gccgaaaagc caccaaacac gactgaatga atcgcaccaa tacgcgcaca
     1501 ggcttgcatc gccaccactg cttcgatgct catcggcata tacaccacga cgcggtcgcc
     1561 tttggttacg cctaaacttt ttaagccatt ggcaaattgg cacaccaaac ggtgtaattc
     1621 ggcataagta actttggtaa ctgtgccatt atcactttca aaaatgactg caattttatg
     1681 ggctaaatcg ggtaaatgac ggtctaaaca gttataagag acgtttaatt caccatcggc
     1741 aaaccactta taaaatggtg ggttgctttg atctaatact tgagtaaacg gtttgtgcca
     1801 cgttaaacgc tcgttggcta atcctgccca aaaaccttca taatcggctt cggcttgttg
     1861 gcacatggct tgatattcgg ccaaactttt aacccgtgcg cctgcgacaa attcagcact
     1921 gggggcaaat tgacgatttt cttgtaatac tgactcaata gacattttct actccttgag
     1981 agactaaatt tttatcaggc caaaagttat aacacgcttt tacgtcattt tttgcttggt
     2041 tagtatgttt ttttaggcca
//
LOCUS       DAOQMB010000415         2060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_36623_length_2060_cov_3.614963, whole genome shotgun sequence.
ACCESSION   DAOQMB010000415 DAOQMB010000000
VERSION     DAOQMB010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36623_length_2060_cov_3.614963"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..1262
                     /locus_tag="PLY05_10575"
     CDS             255..1262
                     /locus_tag="PLY05_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNC03783.1"
                     /translation="MMKIQHHTIANHYVAATLAGAVAQGYVADELLHQAGLSTDIINQ
                     TDIRLPPSQYARLVKHLWRLLDDEFLGLTAHRCPIGSFALMAELLIYSKTLGEALQQC
                     ARFYRLLTHDMLIGLEVKQGQAQLSLTLNQPNHDHQHFLLEFSLVMSSRLASWLIRER
                     IPLQAASFRHSPPAHVHEYPTLFPCTLLFEQSRDALLFDASWLDRPIVRQLSELQTLL
                     PSLPAAFLVKPVFHGSYTQRVRELIQHDLDNFAELEVVAQHFWLTGRTLRRKLISEGS
                     SFQGIKDAIRRDLAIDYLAQGKLSLQEISLNLGFAEASAFIRAFKQWTGYTPRAYQVR
                     G"
     gene            1345..>2060
                     /locus_tag="PLY05_10580"
     CDS             1345..>2060
                     /locus_tag="PLY05_10580"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC03784.1"
                     /translation="MATTLIYGGSGGIGQAIAKRLHQQGQHLHLVGRDAEKLAAFAQT
                     IGASYTCGDVTNPELFAQATAEAGEQLTGLVYAVGNINLKPLRRLTIEDFQQDYAVNS
                     IGAALAAQAAFPVLKKNSSASVVFFSSIASQQGFSNHASISMAKAAVNGLTLALAAEF
                     APHIRVNTIAPSLTATPLAASLTNNPTVAEAIANLHPIPRLGTADDIAALACFLLSSD
                     SSWLTGQILHVDGGRSTLRP"
BASE COUNT          566 a          440 c          452 g          602 t
ORIGIN      
        1 gcataatatt gatagaggca tcgtggccat caaacaagct ggccgccgtg acaaaacgaa
       61 ctttattagt gggtgtatag attgcaggtt tagcaggtgc aagggtcatt taatggccac
      121 tcccatcgtt aacagagctt tatttaataa tagctgagtt tggccaatgg taaaaaaatg
      181 cacaatgtta aaaaggttga attaattgac aaaaacggac aagatgaatg tatgactatt
      241 ttaatgaatg actcatgatg aaaatccaac atcatactat cgccaaccat tatgttgcgg
      301 cgaccttagc aggcgcagtt gctcagggtt atgttgccga tgagttatta catcaggcag
      361 ggctttcaac cgatattatc aatcaaacag acattcgttt gccgccaagc caatatgcaa
      421 gattggtcaa acacctttgg cgattattag acgatgagtt tttaggctta actgcccatc
      481 gttgccccat tggtagtttt gcgctaatgg ccgaattatt aatttatagc aaaaccttgg
      541 gcgaagcctt gcagcaatgt gcgcgttttt atcgtttatt aacccatgat atgttaattg
      601 ggctagaggt taaacaaggt caagcacaat tatccttaac cctgaatcaa cccaaccatg
      661 accaccagca ttttttatta gagttttctt tggtgatgtc tagccgtttg gccagttggc
      721 tgattcgtga acggattcct ttgcaagcgg caagctttag gcactcaccg cctgcccacg
      781 ttcatgaata cccaacgctg tttccttgta ccttgttatt tgaacaaagc cgtgatgctt
      841 tgttgtttga cgccagttgg ctagacaggc cgattgtgcg ccaactctct gagttacaaa
      901 cgttgttacc cagcttacct gccgcttttt tagtaaaacc tgtgtttcat ggcagttata
      961 cccaacgagt acgcgagtta atacaacatg atttggataa ctttgccgag ttggaggtag
     1021 tcgctcaaca tttttggtta acaggccgca ccttgagacg caaattaatc agtgaaggta
     1081 gtagttttca aggcattaaa gatgccatcc gccgagattt agcgattgat tatttggcac
     1141 agggcaagct gtctttgcaa gaaatttcgc taaacttagg ctttgccgaa gccagtgcgt
     1201 ttattcgtgc ctttaaacaa tggacaggtt atacacctag agcctatcaa gtacgtggct
     1261 gaaatttgtc caagctatgt ttgtgtcttt ttattgtttt agcgtcaata attaaaacat
     1321 taatcacaga ctgtattaga catcatggca acaacactta tttatggtgg cagcggcggc
     1381 attggccaag ccattgctaa acgcctacat caacaaggcc aacacctaca tttagtgggg
     1441 cgtgatgctg aaaaattggc agcctttgcc caaacgatag gcgcaagcta tacctgtggt
     1501 gacgtgacaa atcctgaatt atttgcccaa gcaactgccg aagcaggtga acaattgaca
     1561 ggtttggtgt atgcggtggg gaacattaac ctaaaacctt tacgccgact gacgattgaa
     1621 gactttcaac aagattatgc cgtcaacagt attggcgctg ctttagctgc acaagcagct
     1681 tttcccgttt tgaaaaagaa tagctcagcc tccgtagtgt ttttttcgag tattgctagc
     1741 caacaaggtt ttagcaatca tgcgtcaatt agtatggcta aagcggctgt gaatggttta
     1801 actttggctt tagcagcaga gtttgcgcct catattaggg tcaataccat tgcgccttca
     1861 ttaaccgcta cacctttagc cgcttcatta accaataatc ccactgtggc agaagccatt
     1921 gccaatttac accctattcc acgtttaggc acagcagacg atatcgcagc tttagcctgt
     1981 tttttattaa gcagtgatag cagttggctg acagggcaaa ttttgcatgt ggatggtggt
     2041 cgctctacgc ttagacctaa
//
LOCUS       DAOQMB010000416         2058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_36681_length_2058_cov_17.686470, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000416 DAOQMB010000000
VERSION     DAOQMB010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36681_length_2058_cov_17.686470"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..862
                     /locus_tag="PLY05_10585"
     CDS             77..862
                     /locus_tag="PLY05_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNC03785.1"
                     /translation="MKTISSRQNPLIKEINLLANNPAERRKQFKTVLDGVHLVEAALA
                     HGVVLENVFVSEHGLRSVEIANLVDRLENQMSCILVTDNVFNAISPTDHPTGILAVIS
                     WHNPAFVLPTQSCVVLETVQDSGNLGTILRTAAAAGIQHILLSDGCAQAWSPRVLRAA
                     MGAHFSLNIYEKVDLLSVLPAYQGQLLATGLTQAKSLYSLNLNQSTAWLFGSEGQGLS
                     EQVMRLAKQQVIIPMSTGVESLNVAAAVAVCLFEQRRQQMRNA"
     gene            complement(920..1534)
                     /gene="rnt"
                     /locus_tag="PLY05_10590"
     CDS             complement(920..1534)
                     /gene="rnt"
                     /locus_tag="PLY05_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004972676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HNC03786.1"
                     /translation="MPHALAQRFRGYYPVIIDVETGGFNAATDGLLEIAAIPLTLDEQ
                     GLFKPLTALHAHIAPFEGAILNPEALKFTGIDPFNPLRQAENEKDAFRRIFHDLRHWQ
                     KEHDCKRTILVGHNANFDLQFLKAAVERTGLKNANPFHPFSVFDTVSLAGLAYGQTVL
                     AKACISAGIDFQNEQAHSALYDAQKTAELFCLIINKWQSLGGLA"
     gene            complement(1534..>2058)
                     /locus_tag="PLY05_10595"
     CDS             complement(1534..>2058)
                     /locus_tag="PLY05_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009501550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNC03787.1"
                     /translation="VLEHITTADAVQFVQQAGDNIGATITAHHLLFNRNHMLVGGIRP
                     HYYCLPILKRQTHQQALLTAATSGSPKFFLGTDSAPHAIHAKEAACGCAGSYTAHAAI
                     ELYAEAFEQMNALDKLEGFASFYGADFYGLPRNTDTITLVKEEWQVPDNYAFGQSVVV
                     PIRAGDKIAWRVLP"
BASE COUNT          625 a          392 c          442 g          599 t
ORIGIN      
        1 ttgtgtaaaa cttaaatcag atgccaaaaa aggcagacca aaggtctgcc ttttttattt
       61 ttaggaaata aagttaatga aaacaatcag ctcacgccaa aatccactta ttaaagaaat
      121 caatttgtta gcgaataatc cagcagaacg tcgtaagcaa tttaaaacag tcttggatgg
      181 tgtgcattta gtcgaggcgg cactggcaca tggtgtcgtc ttagaaaatg tgtttgttag
      241 tgaacatggt ttgcgctcag ttgaaattgc aaatttagtt gatcgtttag aaaatcaaat
      301 gtcctgtatt ttggtaacag ataacgtatt taatgcgatt agccctacag atcatcctac
      361 gggtatttta gcggttattt cgtggcacaa tcctgctttt gttttgccaa cacaatcttg
      421 tgtggtgtta gaaacagtgc aagattcagg aaatttaggc actattttgc gtactgccgc
      481 agcggcaggt attcaacata tcttattaag tgatggttgt gcgcaagctt ggtcgcctcg
      541 tgtattgcgt gcagcaatgg gggcacattt tagtttgaat atttatgaaa aagttgactt
      601 gttatctgtg cttcctgctt atcaaggaca attgttagca acaggtttaa cacaagcaaa
      661 atcattgtat agtttaaact taaatcaatc gacagcatgg ctatttggca gtgaagggca
      721 agggttatca gaacaagtga tgcgcttggc taaacaacaa gtcattattc ctatgtcaac
      781 aggtgtggag tcgttaaatg ttgccgcagc ggtggcggtg tgtttgtttg agcaaagaag
      841 gcaacaaatg cgaaatgctt agaatttagg ggctgttgaa cagcccctag ttatcatgat
      901 tcataaacgt caacagaccc taggctaagc cgcccaagct ttgccattta ttaataatta
      961 aacaaaataa ttctgctgtt ttttgtgcat catataaagc tgaatgtgct tgttcgtttt
     1021 gaaaatcaat ccctgcactg atacacgctt tggctaatac ggtttgacca taagccaaac
     1081 ccgccaaact gacggtatca aacaccgaaa atggatgaaa cggattagca ttttttagcc
     1141 cagtacgttc aacagccgct tttaaaaatt gcaaatcaaa attggcatta tggccaacca
     1201 aaatggttcg tttacagtca tgttcttttt gccaatggcg taaatcatga aaaatacgtc
     1261 gaaaggcatc tttctcattt tcagcttggc gtaaaggatt aaaagggtca atacctgtaa
     1321 acttaagtgc ttctggattg agtatagccc cctcaaatgg agcaatatgc gcgtgtaaag
     1381 cagttaatgg tttaaataaa ccttgctcat ctaaagtgag aggtatagcg gctatttcta
     1441 ataagccgtc tgtggcggca ttaaagccgc cagtttccac atcaataata acagggtaat
     1501 agcccctaaa acgttgtgcg agtgcgtgtg gcattatggc aatactcgcc acgcaatttt
     1561 atcaccagcg cgaataggta caaccacact ttgaccaaaa gcataattat cagggacttg
     1621 ccattcctct ttgaccaacg taatagtgtc tgtgttacgg ggtaagccat agaagtctgc
     1681 tccatagaag ctggcaaaac cttctaattt atctaaagca ttcatttgtt caaaagcttc
     1741 agcatacagt tctatcgcag catgagcggt ataagaacct gcacaaccac aagcagcttc
     1801 tttggcatga atggcatgag gtgcgctatc tgtacctagg aaaaatttgg ggctaccaga
     1861 tgttgcggca gtcaataagg cttgttgatg tgtttggcgt tttaaaatcg gtaagcaata
     1921 gtagtgaggg cgaataccac caaccaacat atgattacgg ttaaataata aatggtgagc
     1981 ggtaattgtt gcaccaatat tatcacctgc ctgttgcaca aattgcacag catcagcagt
     2041 ggtaatatgt tccaagac
//
LOCUS       DAOQMB010000417         2052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_36890_length_2052_cov_5.310466, whole genome shotgun sequence.
ACCESSION   DAOQMB010000417 DAOQMB010000000
VERSION     DAOQMB010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36890_length_2052_cov_5.310466"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..1087)
                     /locus_tag="PLY05_10600"
     CDS             complement(137..1087)
                     /locus_tag="PLY05_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNC03788.1"
                     /translation="MGFKVLFILMLLLSSVNCAAKTYKCSGNYFGDKPISDACKEINI
                     IGSSQNDSASSKIIRQKHGDVINLSNIPSSSHIHQNSHMVNPQKAVIPFFIDKASNYF
                     EGGNEKYFSHFMSRWNSIDIPIKILHIGDSHVRSGIGAQVSGHVLQGERKAVVYRSKG
                     LNGATLQGVDKVLNLESELGYFNPDLVVLDFGSNELHSRQLALSSSLEQAMESAISRI
                     RAVKPDTVIVFAAPQDMNIKQAVSVQEYTANMRRIALDNDCLLWDWHRVAGGVGAMRS
                     SWYVNGLARADFVHLTAKGYRVKGELFANALLNTIKQYQR"
     gene            complement(1087..1734)
                     /locus_tag="PLY05_10605"
     CDS             complement(1087..1734)
                     /locus_tag="PLY05_10605"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HNC03789.1"
                     /translation="MKGILWAVSAACLLSSFSHIAIAQNPTKPKDNITINLDYSLDLL
                     EFKIEPKDSYRYIKAPLTNKPTEATQALFKKYPSHHMVWQVFSEICNLGIREPKIVMS
                     QALLETGHFKAKFLMERNNLFGFRAQRYLSFSNWKESVRYYRDWQVRRYKPHDKNYYA
                     FLNRIRYGAPTYSQHLQQTSLWKHDCSVLNQVSTVDQLALFQVPLKKEAVNQFGG"
BASE COUNT          614 a          449 c          355 g          634 t
ORIGIN      
        1 caccgccttt gcgggcgtgc cggttcgagt ccggctctag gcaccaagat tccaagatag
       61 tcaatatctt ttaaaaagcc ttgatttgta atgaatcagg gcttttttat tgtccatagc
      121 gtttatgata ttacatttaa cgttgatatt gcttaatcgt atttaacaaa gcattagcaa
      181 acaactcgcc tttcacacga taacctttgg ctgtcaaatg cacaaaatcc gcacgagcca
      241 agccattgac ataccacgac gaccgcatag cccctacacc acccgccacg cggtgccagt
      301 cccacaacaa gcagtcatta tctagcgcga ttctgcgcat attcgctgta tactcttgca
      361 cagataccgc ctgtttaatg ttcatgtctt gcggtgccgc aaacacaatc actgtatcag
      421 gtttaacggc gcgaatacgc gaaatagcac tttccatcgc ctgctctaat gaagatgaca
      481 acgccaactg ccgactatgt aactcattac tgccaaaatc taacaccact aaatcaggat
      541 taaaatagcc aagctcactc tctaaattaa gaaccttatc aaccccttgt aatgtcgctc
      601 cgttcagtcc tttactgcga taaacgactg ctttgcgctc cccttgtaac acatggcctg
      661 atacttgcgc accaatgcca cttcgtacat gagaatcacc aatatgaagt attttaatgg
      721 gaatatctat agagttccaa cggctcataa aatgcgaaaa atatttctca ttgccgcctt
      781 caaaataatt actcgcctta tcaataaaaa atggtatcac cgctttttgt ggatttacca
      841 tatggctgtt ttggtgtatg tgggatgatg aaggaatatt gctcaggtta atcacgtcac
      901 cgtgtttttg tcggataatt tttgaggagg ctgaatcatt ttggctgctg ccgatgatat
      961 ttatctcttt acaagcatca ctaatcggtt tatcaccaaa ataattgcca ctgcatttat
     1021 aggtttttgc cgcacagtta acactcgaca acaacaacat cagtatgaat aaaaccttaa
     1081 atcccattat ccaccaaatt gattcacagc ttctttttta agtggcactt gaaacaacgc
     1141 caattgatca acagtagaca cttgatttaa caccgaacaa tcgtgcttcc ataaagacgt
     1201 ttgttgtaaa tgctggctat aagtgggggc accataacga atacggttta aaaaagcata
     1261 ataattctta tcgtgaggct tatatctgcg tacttgccaa tcacggtaat aacgcacgct
     1321 ttctttccag ttactaaaac ttaaataacg ctgcgctcta aagccaaata aattattgcg
     1381 ctccattaaa aatttagcct taaagtgccc agtttctaac aaggcttgac tcatgacaat
     1441 tttcggctcc cgaatgccta aattgcaaat ttctgaaaaa acttgccata ccatatgatg
     1501 agaaggatat tttttaaaca aggcttgtgt agcctctgtg ggtttgtttg tgagtggtgc
     1561 tttaatataa cgataactgt ctttgggctc tattttaaac tctagtaaat ctagagaata
     1621 atctaagtta atggttatat tgtctttagg ttttgtggga ttttgtgcga tagcgatgtg
     1681 agaaaacgat gacaacaaac aagcagccga tacagcccat aatatacctt tcataaacaa
     1741 acctcaataa aagcattaca ctgagtgtag agtacgtttt tcctctaacc aaacataacc
     1801 ttttgtttca catacaaaaa aatattttta ttatttttca cctgccaatc ttacaagagg
     1861 caggctaaaa atgatgcttt tttcgtcaga caattgtaag attagttgtc gttattcggc
     1921 cattttggtc aagttttctg cttttgatgg gctgttttca tctaacattt tccatagttt
     1981 ttgcaaaaac aagttcgcac tatatttgta gccctctgct gtgagatgaa ttaaatctcg
     2041 ttgagctaat ac
//
LOCUS       DAOQMB010000418         2045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37141_length_2045_cov_5.511055, whole genome shotgun sequence.
ACCESSION   DAOQMB010000418 DAOQMB010000000
VERSION     DAOQMB010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37141_length_2045_cov_5.511055"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(375..653)
                     /locus_tag="PLY05_10610"
     CDS             complement(375..653)
                     /locus_tag="PLY05_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HNC03790.1"
                     /translation="MLTIKVKKQFKKDLKKALLDPKRNTDLLKKLIDEHLIHTGTVPV
                     EYQPHPLTGNWKPHMECHIQPDFLLIWDVDWQAKHLILVRCGSHSELF"
     gene            complement(640..906)
                     /locus_tag="PLY05_10615"
     CDS             complement(640..906)
                     /locus_tag="PLY05_10615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNC03791.1"
                     /translation="MHTHKAEIVRARIAPDIKHNAEQVLASLGMSMSDAIRIFVSQVA
                     IRQSFPIELKTPNQITLEAMQAEAEQAEYTSASTLFDELSHVDD"
     gene            complement(1139..1885)
                     /locus_tag="PLY05_10620"
     CDS             complement(1139..1885)
                     /locus_tag="PLY05_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNC03792.1"
                     /translation="MATTNSNKLTDTAVKSAKPKDAPYKLSDGGGMYLLIKPNSGKYW
                     HLAYRFNNKQKLLALGVYPEVSLKEARLKRDQARKLLQDGTDPSEAKKEEKRLSSLQS
                     DNTFEAIANEWLSKRTNLTATTVKSYQRYLSYAFAAFGHKPVNEVTSPDVLALCRTLE
                     ARGTTETAHRIKMTCGLVFRYAIATGRTTYEPTQALRGALEPVVTTHQAAITDPSQVA
                     RLMQDIQGYEGHYTTVYALKLAPLVFVLPR"
BASE COUNT          594 a          399 c          406 g          646 t
ORIGIN      
        1 ggctttggac gaaaacaaat ctgaatgatg acgaagaaaa actacgattc tctgaatagc
       61 tgtggtttaa ctatacaaaa atagctctaa gcattggcat ataaggcttt gtgcgtgttt
      121 acacggtgcg cgatgttgtc acctgttgcg tactgtggcg gtggtttttt aattacgttg
      181 acaaggtgac ttatcaaaaa tgcttctaac cattgatacg gttagtttta acggtctttt
      241 cttgtttcca attcgtcaac gtaggtcaac gtaaactact ttttagtgtg tttaaaccat
      301 caatataagc ctaaaacgtg tcgattttga gccagtaaat aaacatgagg gcaagcggtc
      361 ttttgtataa aaaactaaaa taactctgaa tgactgccgc atctgactaa aattaaatgt
      421 ttagcttgcc agtcaacatc ccaaatcaat aaaaaatcag gctgtatatg acattccata
      481 tgaggcttcc aattacctgt taaagggtgt ggctgatatt caactggtac tgtacctgta
      541 tgtattaaat gctcgtcaat caacttttta agtaagtctg tattgcgttt agggtctagt
      601 aatgcttttt ttaaatcctt tttaaattgc tttttaacct taatcgtcaa catggcttaa
      661 ctcatcaaac aaagtactcg cgctggtgta ttctgcttgc tcagcttcgg cctgcattgc
      721 ttctaaggta atctgattag gcgtttttag ttcaataggg aatgattgtc taatagctac
      781 ctgactaaca aagatacgaa tagcatcgga catactcata cctaaagaag ctaatacttg
      841 ctctgcatta tgtttaatat cgggagcaat acgcgccctt acaatttcag ctttatgagt
      901 gtgcatggct tttctcctat aatgatttta cactgtagca caaaaaggct acagtgtaaa
      961 aaagatacac tcaacaaaaa aatgggtatg cctaaaactg gatacacccc ttaacctcaa
     1021 aaaaatgata accctaaatt aaaaattagc ctcattttct aatcgttgcc cctcactgca
     1081 atacctacca agactaggct tacacacctt acaagctgtc cagtgattga taaatgtctc
     1141 atctagggag aacaaatacc aaaggggcta atttaagcgc gtaaacagta gtgtaatgcc
     1201 cctcatagcc ttgtatgtcc tgcatcaatc gggcaacttg cgaggggtca gtaatcgcgg
     1261 cttgatgcgt ggtgacaact ggctctaatg cacctctaag ggcttgtgta ggctcataag
     1321 tggtgcggcc tgtcgcaata gcatatctaa acactaaacc acaggtcatt ttgatacggt
     1381 gggctgtttc tgttgtgcct cttgcttcaa gagtacgaca caaggccaaa acatcggggc
     1441 tagttacttc attaactggt ttatggccaa aggctgcaaa agcataggat aaataacgct
     1501 gataactttt aacggtggta gcggtgagat tagtacgctt gcttagccat tcattcgcta
     1561 tggcttcaaa agtgttgtct gattgcaaag aagataaacg cttttcttct tttttggctt
     1621 cgcttgggtc tgtgccgtct tgtaatagtt ttcgggcttg gtcgcgtttt aaacgtgctt
     1681 cttttagcga gacttcggga tatacgccta aagctaacaa tttttgcttg ttattgaatc
     1741 ggtaagctaa atgccagtat ttgccactat taggctttat gagcaaatac ataccgccac
     1801 catctgatag cttgtaaggt gcatctttag gctttgcact ttttacggca gtgtcggtta
     1861 atttattgct gttggtagtt gccatgttgg tatctttttt gatggtagtt gactgatacc
     1921 aacgaaaata ccaacaaaaa acttggctta caatatctat aatgaacact atagacaata
     1981 aaaaagcctg taagtattgc tactacaggc tttcttgttc taggctgaac accttagaac
     2041 tatat
//
LOCUS       DAOQMB010000419         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37163_length_2044_cov_17.373555, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000419 DAOQMB010000000
VERSION     DAOQMB010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37163_length_2044_cov_17.373555"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..976)
                     /locus_tag="PLY05_10625"
     CDS             complement(38..976)
                     /locus_tag="PLY05_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004695578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HNC03793.1"
                     /translation="MAGSSLLALIDDIATLLDDVAAMTKVAAKKTSGVLGDDLALNAQ
                     QVAGVKADRELPVVWAVAKGSLKNKAILVPAALLISAFANWAIMPLLMLGGLFLCFEG
                     VEKLIHPLLHSHDEDEQHHQELVQAVTNPEVDILAFEKEKVTGAIRTDFILSAEIIVI
                     TLGTVAQTTFINQVIVLIGIAILMTVGVYGLVAGIVRLDDMGLHLTQQANAAKQQLGR
                     FLLALAPKLMKFLSIAGTAAMFMVGGSILVHGLPVVHHAIEHSAETASHIAVVGGLLK
                     FVVGMLAEMLVGIVAGLAAVIVMTGVSKVLPKKVEA"
     gene            complement(980..1876)
                     /locus_tag="PLY05_10630"
     CDS             complement(980..1876)
                     /locus_tag="PLY05_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016650967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC03794.1"
                     /translation="MLKKLLAQGLRKIFKLTPQAHIYSPPLAEDEQAIELTYLGTAGF
                     IIKNQQRTVVLDPYLSRIGVTELLRPLHTNTALLKKYIPKANDVLIGHAHYDHILDAP
                     DLCKQTGARLIGSRATCMVGQAAGLPESQLVETEGREDIACGAWTVRGLPSIHGKAIM
                     GRIPIPGDILSPPVWPPKMLDLKHGLVLNWLVDTGKLKIVHIDSADFIAEELKGVQAD
                     VVCLCAIGRKYRPNYVKEVVELLKPKWIVPCHWDTMFTPFEQEPDYIPSVDLAGFVQE
                     IKDAGVEPIVMPMRGKQWFKRA"
BASE COUNT          630 a          466 c          349 g          599 t
ORIGIN      
        1 tgtcagccac agcattatgc ggctgacaat agatgtatta agcctctact tttttaggca
       61 atactttgct aacacctgtc ataactatca ctgcggctaa acctgcgaca ataccgacta
      121 acatttcagc taacatacca acaacaaact ttaataagcc accaaccaca gcaatatgtg
      181 aagcagtttc tgcactgtgt tcaatcgcat gatggacaac aggcaaacca tgaaccaaga
      241 tgctaccacc gaccataaac atcgctgctg tgcctgcaat cgataaaaac ttcattaatt
      301 taggggctaa tgccaataaa aagcggccta attgttgttt ggcagcattg gcttgttgag
      361 tcaggtgtaa acccatatca tctaagcgaa caatacctgc caccaaacca taaaccccta
      421 ccgtcattaa aatcgcaata ccaattaaaa caatcacttg attaataaat gtggtttgtg
      481 ccactgtgcc taaggtaata acaataattt ctgctgataa aataaaatcg gtacgaattg
      541 cacctgtcac tttttctttt tcaaacgcca aaatatccac ttcgggattc gtcacggctt
      601 gtactaactc ttggtgatgt tgttcatctt catcatgact atgcaataag ggatgaatca
      661 gtttctccac gccttcaaaa cacaaaaaca aaccacccaa cattaataac ggcataatcg
      721 cccaattagc aaaggcacta attaatagtg cggctggcac taaaatcgct ttatttttta
      781 acgatccctt agcgactgcc caaactactg gcaactctct gtcagctttt acccctgcta
      841 cttgttgggc atttaaggct aaatcatctc ccaagacgcc tgatgttttt ttggctgcga
      901 ctttcgtcat tgctgccaca tcatctaata atgtggcaat atcatcaatt aatgctaata
      961 ggctactacc tgccatattt tatgctctct taaaccattg ttttccacgc atgggcatga
     1021 cgataggctc tacccctgca tctttaattt cttgcacaaa gcctgccaaa tcaacactcg
     1081 gaatataatc tggctcttgt tcaaacgggg taaacatcgt atcccaatga caaggcacaa
     1141 tccatttagg ctttaacaat tcaaccactt ctttgacata attaggccga tattttcgac
     1201 caatcgcaca caaacaaaca acatctgctt gcacaccttt taattcttcg gcaataaaat
     1261 cagccgaatc aatatgaaca atttttaatt tgcctgtatc tactaaccaa tttaaaacca
     1321 aaccatgttt taaatcgagc atttttggcg gccaaacagg tggtgacaaa atatcaccag
     1381 ggataggaat acgccccata atcgctttgc catgaatcga aggtaaacca cgcactgtcc
     1441 atgcaccaca ggcaatatct tcacggcctt cggtttcaac taattgcgac tcaggtaaac
     1501 ctgctgcttg accaaccata catgttgccc gtgaaccaat taaccgtgcg cctgtttgtt
     1561 tacataaatc tggtgcatcc aaaatatggt cataatgggc atgaccaatc aacacatcat
     1621 tggctttggg aatatatttc tttaataatg ctgtattagt atgtaatggc cgtaagagtt
     1681 cagtgacacc aattcgactc aaataagggt ctaagaccac agttctttgt tgatttttaa
     1741 taataaaacc cgctgtacct aagtaggtta attcgatggc ttgttcatct tcggctaaag
     1801 gaggactata aatatgcgct tgtggtgtta atttaaagat tttgcgtaag ccttgagcga
     1861 gtaacttttt tagcatagca cctttatcca tgatgattat caaattaagt ttaatgcttt
     1921 atgatgattg tttgcacctt agacgattaa cgtaacaaag acaagtgagt aaatgatgac
     1981 aaagatcggt tttattggca tgggtttaat gggtagtcgg atgacttctc gtttgttaca
     2041 agcg
//
LOCUS       DAOQMB010000420         2044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37169_length_2044_cov_5.922071, whole genome shotgun sequence.
ACCESSION   DAOQMB010000420 DAOQMB010000000
VERSION     DAOQMB010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37169_length_2044_cov_5.922071"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PLY05_10635"
     CDS             <1..377
                     /locus_tag="PLY05_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004663455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC03795.1"
                     /translation="KQKEGKISIIWNHAVDEVLGDDSGVTGVRVKSTVDGSTQVVDVA
                     GVFIAIGHKPNTDIFAGKLAMKNGYLTVKSGTEGNATMTSVEGVFAAGDVADHIYRQA
                     ITSAGSGCMAALDAEKYLDNLK"
     gene            complement(420..>2044)
                     /locus_tag="PLY05_10640"
     CDS             complement(420..>2044)
                     /locus_tag="PLY05_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03796.1"
                     /translation="AFKDGSYSYEVIFNNNALYEINLSLTSFLGQFTPTATTQPTTET
                     CASKNLAVATLSSIADYNGDYKEAGTVVFNLNTATAKASVKGTEANITEVCGPTVITS
                     GTNHVLITDKGNVTLFKTTAGVQSAEGYEFVNKNNTFYGEKAGAALTNLAASGTATGT
                     ISNVQYNKDGTTTGGTGQVRSKVYTFSGDLNGTYTIKIKDTAQNLRYDVNGTNGKPSS
                     VNLYSAESSASPLFVYTCDDSSCLSKVSLTQSSTGTTVKFTDMKMRGVYQAQYDAIIN
                     GQSLLTANTLCESTGHDTKLGFKNAPSDFCSFSYTYGTSTAISSPDIYTFKDSTDPMA
                     NAKVTVLNGSVQTFQLENSKYAYACGVGNMPACVGITIQSNTNSNIEFKFANVQVANV
                     DSTKQGMIIDSGLLMHKKATQTTTPVNLAACLTQKTSVSAINCSAQVIPDFTLNMRSS
                     VTLINNSCTVSKVGQILTATSGAISISAKLNEETIDLARVDSTYSLVNATDVVNNLSN
                     TVQITYSSAGAITNVIAQGNGGGQGFFCGPNQ"
BASE COUNT          585 a          383 c          414 g          662 t
ORIGIN      
        1 ctaaacaaaa agaaggcaaa attagcatta tttggaatca tgccgttgat gaagtgttag
       61 gtgatgattc tggtgtgact ggtgtgcgtg ttaaaagtac ggttgatggt agcacacaag
      121 ttgtcgatgt cgcaggtgta tttattgcca tcggacacaa gccaaatacc gatatttttg
      181 caggcaaatt agccatgaaa aatggctatt taacggtaaa aagcggcact gaaggcaacg
      241 caacaatgac ttctgttgag ggtgtatttg cagcaggcga tgtggctgac cacatttatc
      301 gtcaagcaat tacctcggca ggttcgggct gtatggctgc gttagatgcc gaaaagtatt
      361 tagataattt gaagtaaaat ataaagaggt tagctttagc taacctcttt ttttgcacct
      421 tactgattag ggccacagaa aaagccttgg cctcctccat taccttgtgc gataacattt
      481 gttatagcac ctgcactgct gtatgttatt tgtacagtat tagacagatt attgactaca
      541 tccgtagcat ttactagaga atatgtactg tctactctag caagatctat tgtttcctca
      601 ttgagttttg cagaaataga aattgcccca gatgtagcgg ttaaaatttg cccaacttta
      661 ctaacagtgc aactgttatt aattagtgta acggagctac gcatatttaa tgtaaaatct
      721 ggaatcacct gagcactaca gttaatagca ctaacactag ttttttgtgt taagcaagct
      781 gctaaattaa ctggagtcgt cgtttgtgta gccttcttat gcatcaacaa accactatca
      841 ataatcattc cttgtttagt agagtcaaca ttggcaactt gcacattagc aaacttgaat
      901 tctatgttgc tgttcgtatt actttgaatg gtaattccca cacaagctgg catattgcct
      961 acaccacaag cataagcata tttactattt tctaattgga atgtttgtac actaccattt
     1021 agaacggtta cctttgcatt tgccattgga tcagttgaat ctttaaaagt gtaaatatca
     1081 ggcgagctta tcgctgtcga tgtgccatag gtataactaa aactacaaaa atcactgggt
     1141 gcatttttaa agcctaattt ggtatcgtgg cctgtagatt cgcataaggt gttagcagta
     1201 agcagagatt gaccattgat aatggcatca tactgagctt ggtaaacacc acgcattttc
     1261 atgtcagtaa atttgacagt cgttcctgta gagctttgag ttaaactcac tttgctaaga
     1321 caactgctat catcacaagt ataaacaaac aatggtgaag cagaactttc tgcagaatat
     1381 aaatttacac tgctaggttt gccgtttgtg ccatttacgt cataacgtaa attttgtgcg
     1441 gtgtctttga ttttgatggt gtaagtgcca tttaaatcgc cagaaaaagt gtagactttt
     1501 gagcgaacct gacctgtacc acctgttgtt gtaccgtctt tgttgtactg cacattggaa
     1561 atggtgcctg ttgctgtgcc actggctgct aggttagtta aagctgcacc cgctttttca
     1621 ccataaaacg tattgttttt gttgacaaac tcataacctt ctgcactttg tacacctgcg
     1681 gttgttttaa ataaagtgac attaccttta tcggtaatta acacatgatt tgtgccgcta
     1741 gttatcactg ttgggccgca tacttcagta atattagcct ctgtgccttt tacagaagct
     1801 ttggctgtgg ccgtatttaa attaaatacc acagtacccg cctctttata atcgccattg
     1861 taatcagcaa tgcttgaaag tgttgcgaca gctagatttt tgctggcaca ggtttcggtt
     1921 gttggttgtg ttgttgctgt cggtgtaaat tggccaagaa acgatgtcaa tgacaaatta
     1981 atttcataaa gggcattatt attaaaaata acctcatagc tatagctacc atctttaaag
     2041 gcat
//
LOCUS       DAOQMB010000421         2042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37238_length_2042_cov_8.137896, whole genome shotgun sequence.
ACCESSION   DAOQMB010000421 DAOQMB010000000
VERSION     DAOQMB010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37238_length_2042_cov_8.137896"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="PLY05_10645"
     CDS             complement(<1..584)
                     /locus_tag="PLY05_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03797.1"
                     /translation="MNKYLSSPFLTSEKIATVKMQFLPVATATVATVVTLEQKTGFED
                     DKKLSSVTKVTSVTVTDSENQKKTLTSQNGNFGFLTELNKISYLTPLNLCERDYLVTK
                     PKTRPSDVKKSHLTNNSKVYVRVNSKSADSSQLAKINQLSFFEGFNCLDLVAVTGNNA
                     RQELRKLLTTVQYHHTLVDVFLCLSFANCLFTGE"
     gene            complement(577..846)
                     /locus_tag="PLY05_10650"
     CDS             complement(577..846)
                     /locus_tag="PLY05_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03798.1"
                     /translation="MATKTKAKKPPLLVWQPRGKEQPSPTIVRPFNVKAADRLPIPIY
                     YLNKTGAWTPIENGFAIPRDRIQDLIDVLTQALAMPYPDKEQPHE"
     gene            complement(847..1227)
                     /locus_tag="PLY05_10655"
     CDS             complement(847..1227)
                     /locus_tag="PLY05_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03799.1"
                     /translation="MDNPTQRITPLISSLETTLEQCTTALNGGGDVAQVIHCLTTIKQ
                     ALAKLRPVIDSLVEAEEQQSLCLFGINDAAIELVLSLDEKQPNYKPRPHAENIELIHS
                     ILECLPDAPPPDNVVNMPVKKEVH"
     gene            complement(1230..1484)
                     /locus_tag="PLY05_10660"
     CDS             complement(1230..1484)
                     /locus_tag="PLY05_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HNC03800.1"
                     /translation="MSVIKPKNPAPYTAETTPQIRFIRKKEVMHRTGLPSSTIYDLMA
                     KGLFPQKITLAGGKAVAWLESDIEQWLKQCLENSTNQGAA"
     gene            complement(1795..>2042)
                     /locus_tag="PLY05_10665"
     CDS             complement(1795..>2042)
                     /locus_tag="PLY05_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HNC03801.1"
                     /translation="SEQMCGHGFRAMARTILDEVLDYPIEVIEMQLAHAVKDANGRAY
                     NRTKYREQRHKMMQHWSDYLDGLQVDNVVAVKFGNGG"
BASE COUNT          559 a          395 c          412 g          676 t
ORIGIN      
        1 aattcacctg taaacaggca attggcaaat gataggcata aaaaaacatc aactaacgtg
       61 tgatgatatt gtacggttgt taagagtttt cgaagctctt gccgcgcatt attgccagtc
      121 accgccacca agtcaagaca attaaacccc tcaaaaaatg ataattgatt gatttttgct
      181 aattgtgacg agtccgcact tttactattt acccttacat aaaccttact attatttgtg
      241 aggtgagatt ttttcacatc actaggtcta gttttcggct tagtcaccaa ataatcacgc
      301 tcacacaaat ttaagggcgt taagtaactg attttattta attctgtcag aaaaccaaaa
      361 ttaccgtttt gactggtaag agtttttttc tgattttcac tatctgtgac tgtgacactt
      421 gtgacttttg tgacagatga tagtttttta tcatcttcaa accctgtttt ttgttcaagt
      481 gtcacaactg tcgctactgt cgcggtcgca acaggtaaga attgcatttt tacggtagct
      541 attttctcgc tcgtcaaaaa tggggagctt agatatttat tcatggggtt gctccttatc
      601 gggataaggc attgctaagg cttgagtcaa aacatcaatc aggtcttgaa tacggtctct
      661 aggaatagca aaaccgtttt ctatgggtgt ccatgcgcct gtcttattta ggtaataaat
      721 ggggataggt agcctatccg ccgccttaac attaaacggc ctaacaatgg tcgggcttgg
      781 ctgctctttg ccacgtggtt gccataccaa caaaggcggt tttttggctt tagttttagt
      841 tgccatttag tgaacctcct ttttaacagg catattcacc acgttatcgg gtggcggtgc
      901 atcgggtaaa cactccagta ttgaatgaat aagttctatg ttttcagcgt gtggacgtgg
      961 tttgtaattg ggctgttttt catctaagga caaaaccaat tcaatggccg cgtcattgat
     1021 tccaaatagg cataaagact gttgttcctc agcctctacc aagctatcaa taacaggccg
     1081 taacttggct aaagcctgtt taatggtggt taagcagtga atgacctgtg caacatcacc
     1141 gccaccattt aaggcggtgg tgcattgctc tagggtggtt tctaggctgc taataagggg
     1201 ggtaatgcgt tgagtagggt tatccatgat taagcggctc cttgattagt tgaattttca
     1261 aggcattgct ttaaccattg ctctatatca ctttcaagcc aagcaacggc tttacctcct
     1321 gccagggtga ttttttgagg aaataagcct ttggccatta agtcatagat agttgatgac
     1381 ggtaggccag tacggtgcat cacttctttt ttacgaataa agcgaatttg tggggtggtc
     1441 tcagcggtat aaggtgctgg gttctttggt ttaataaccg acatgataaa agcctctaag
     1501 gtgttcaata cagaacgagg cgcatattat ttatcaagtt ttttagggtc ttttttaaaa
     1561 gttccagtag atgttaaaaa acaatcaact tttataaaat tgtggataac taagggtttt
     1621 attgtgtttg cctgtggata agtcaaacac aaaatattgt ttttatttta atttgttatt
     1681 gtatgttttt tatacatatc tgtatgaaaa atatacacaa aaagagcttt taagctgttt
     1741 ttacacaaat acacctttta tcgtcaaaaa ataattttga tgatttttta agaattagcc
     1801 cccattgcca aacttaaccg ccaccacatt atcaacctgt aaaccgtcta agtaatcgct
     1861 ccagtgttgc atcatcttat gacgttgctc tcggtacttg gtacggttat aggctctacc
     1921 gttggcatct ttaacggcat gggcaagctg catctctata acttcaatcg gataatctaa
     1981 aacctcgtct aatatggttc tagccattgc cctaaagcca tgcccacaca tttgctcact
     2041 gg
//
LOCUS       DAOQMB010000422         2040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37295_length_2040_cov_8.528967, whole genome shotgun sequence.
ACCESSION   DAOQMB010000422 DAOQMB010000000
VERSION     DAOQMB010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37295_length_2040_cov_8.528967"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..416
                     /locus_tag="PLY05_10670"
     CDS             45..416
                     /locus_tag="PLY05_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC03802.1"
                     /translation="MSINAPAKAPAFCKVQVRFMLNDEIALGSGKVELLAAIAETGSI
                     AAAGRKMNMSYRRAWLLVETMNRCFMSPLVVSCKGGNHGGGAEVTELGQQVITLYQEL
                     LATIQSQTQQQQQQLLALLKP"
     gene            complement(405..1103)
                     /gene="modB"
                     /locus_tag="PLY05_10675"
     CDS             complement(405..1103)
                     /gene="modB"
                     /locus_tag="PLY05_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNC03803.1"
                     /translation="MLITVELWQSLWLTIKLAMSATILLMLLTIPLSYYLYYGRSRWW
                     LLIELIVTLPMVLPPTVIGFYLLILFSPQHWFGKVWQQVFDSTLVFSFSGLVIASVLY
                     SLPFALQPMQSAFKQVDESLIEGAVALGATPKQVFYTIVIPVARYGIFSGALLSFAHI
                     LGEFGVVLMLGGSIPNETRVASIALYDELQKLNYPAAHQFALLLLILSAMMLFAIKIW
                     QRSQESRPRLKLGL"
     gene            complement(1110..1856)
                     /gene="modA"
                     /locus_tag="PLY05_10680"
     CDS             complement(1110..1856)
                     /gene="modA"
                     /locus_tag="PLY05_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC03804.1"
                     /translation="MILMLTLLLSITSAWAANSLTIAAAANLVYCINDLNKVFNQQYP
                     KVRVTVTTGASGNFFAQIQQNAPYELFLSADTLYPQQLVDRQLADSKSLKVYTLGQVV
                     LWSNKPDIKVSQGLDILKATEIKRIAIANPDTAPYGMAAKLALQNNGLWDSVQRKLVF
                     AESIAQTMQFVQTQNAEVGIVALSLLKAPQLQQQGHYWLIPQEDYPPLKQAMVVTKQG
                     QKNEWAYRYAAFLQSAKAREIFSQYGFLQP"
BASE COUNT          684 a          415 c          352 g          589 t
ORIGIN      
        1 acaaccgcaa cagataaaga ctataaaact agcttagagt ttagatgtca ataaacgccc
       61 cagccaaagc acctgctttt tgtaaagtgc aggtgcgttt tatgcttaat gatgaaattg
      121 ctttaggttc tggcaaggta gaattacttg ccgccattgc cgaaacaggc tctattgcgg
      181 cagcaggccg caaaatgaat atgagctatc gtcgagcatg gttattagtc gaaaccatga
      241 atcgttgctt tatgagtcct ttagttgttt cttgtaaagg cggtaatcat ggtggaggtg
      301 cagaagtcac tgaattaggc caacaagtca tcactttata ccaagaattg ttagcaacca
      361 tccaatctca aacacagcag caacaacaac aattattagc tttattaaag ccctaatttt
      421 aatctcggtc gggactcttg cgagcgttgc catattttga tagcgaataa catcatagcc
      481 gataaaatta ataacaataa tgcaaactga tgcgcagcag gataatttaa tttttgtaac
      541 tcatcataca aagcgataga ggctacacga gtttcattag gaatacttcc tcctaacatt
      601 aacacaacac caaactcacc taaaatatgt gcaaaactta acagtgctcc actaaaaata
      661 ccgtagcgag caactggaat gacgatagta taaaatactt gtttaggcgt cgctcctaat
      721 gctacagcac cttctattaa gctttcatct acttgtttaa aggcactttg catgggttgt
      781 aaagcaaaag gcaagctata caataccgaa gcaatcacta gtccagaaaa agagaaaact
      841 aaagtgctat caaaaacttg ctgccatact ttgccaaacc aatgttgtgg tgaaaatagt
      901 attaataaat aaaagccgat cactgttggt ggtaatacca taggcagagt gacaatcaat
      961 tctattaaca accaccaacg actacgccca taatacaggt aatagcttaa aggaatcgtt
     1021 aataacatta ataaaatcgt agcactcatg gctaacttta ttgttagcca taacgactgc
     1081 cataattcaa cggtaatcaa catactgggt tatggctgta aaaaaccata ttgactaaaa
     1141 atttcccttg ctttagcaga ttgcaaaaat gcagcataac gatatgccca ctcatttttt
     1201 tgaccttgtt tagtcaccac cattgcttgt tttaatgggg gataatcttc ttgaggaatg
     1261 agccaatagt gtccttgttg ttgtaattgt ggtgctttta acaacgataa ggccacaata
     1321 cctacctctg cattttgagt ttgtacaaac tgcatagttt gagcaatact ttcggcaaag
     1381 actaattttc tttggacaga gtcccataag ccattatttt gtaaggccaa tttagcagcc
     1441 ataccataag gagcagtatc aggattagca atcgcaatcc gcttaatttc tgtagctttt
     1501 aaaatatcta gcccctgact aaccttaata tcaggcttat tactccacaa tacaacctgc
     1561 cctaatgtat aaactttcaa acttttacta tctgctaatt gtctgtcaac taattgctgt
     1621 ggatacaaag tatccgcaga taaaaacaat tcataaggag cattttgctg aatttgagca
     1681 aaaaaattac ctgaagcacc tgtagtcact gttactctaa ctttaggata ttgctgatta
     1741 aaaactttat tcaaatcatt tatgcaatat actaaattcg ctgctgcggc aatcgtcaaa
     1801 ctattcgccg cccaagcaga tgttatggat aataacaaag ttaacattaa tatcatgcga
     1861 gacaacatac atgccaccga tatatctcat taaacatatc ggctattgta ggcaaacatt
     1921 ttacaaaaag caatttacgg taaaatggcc attgctggat taactaacca cccttcaacc
     1981 attagcacgg cggcagttga gtctaaagaa ggtaatttgc ctttgcgttg ttgaccaata
//
LOCUS       DAOQMB010000423         2038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37374_length_2038_cov_16.029249, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000423 DAOQMB010000000
VERSION     DAOQMB010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37374_length_2038_cov_16.029249"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /gene="parE"
                     /locus_tag="PLY05_10685"
     CDS             complement(<1..1493)
                     /gene="parE"
                     /locus_tag="PLY05_10685"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNC03805.1"
                     /translation="MTTYNADAIEVLSGLDPVRRRPGMYTDTSRPNHLAQEVIDNSVD
                     EAMAGHACQIQVILHEDNSLEVIDDGRGMPVDMHPEEKMSGVELILTKLHAGGKFSNK
                     NYQFSGGLHGVGISVVNALSSQVQVKVKREGQEYAISFANGNKSKELEVIGSVPKRQT
                     GTSVRFWSDPQYFDNPKFNTKALKHLLKAKAVLCPNLQIKWIDKQAKEEETWYYEDGL
                     RAYLIDSCKDFECLPAEPFVGDFKGEHAACEFAVLWQPEGGETIQESYVNLIPTVQHG
                     THVNGLRQGLTDAMREFCEFRNLLPRNVKLAPEDVWERVAFVLSMKMQEAQFSGQTKE
                     RLSSREASVFVQGVVKDAFSLWLNQHPDVGQQLAELAIASANKRLRASKKIERKKITQ
                     GPALPGKLADCAGQDVMRGELFLVEGDSAGGSAKQARDKEFQAIMPLRGKILNTWEVA
                     ADQVLASQEVHDIAVAIGVDPANDDLTGLRYGKICILADADSDGLHIA"
     gene            complement(1490..>2038)
                     /locus_tag="PLY05_10690"
     CDS             complement(1490..>2038)
                     /locus_tag="PLY05_10690"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HNC03806.1"
                     /translation="IHGFNSSPQSHKAGLLRQWLAQHRPDIQFITPDLNIFPQQAINL
                     LVQLVQQYPQAGLLGSSLGGFYATWLNQHYGNKAVLINPAVKAYDLLRSLLGEQRNYH
                     TGKGYVLTQAQVDELLAIEVDALKYPERLWILLQTADETLDYRLALAKYPLSPMLVEE
                     GGNHAFDSFEHHIPAIIDFLYL"
BASE COUNT          602 a          491 c          375 g          570 t
ORIGIN      
        1 gcaatatgca aaccatcgga gtcggcatcc gccaaaatac aaattttgcc ataacgtaac
       61 cctgtcaaat catcattggc agggtctaca ccaattgcaa ccgcaatatc atggacttct
      121 tgagaagcca atacttggtc tgccgccact tcccatgtgt ttagaatctt accacgcaac
      181 ggcataatcg cctgaaactc tttgtcacgc gcttgtttgg ccgaaccacc tgccgagtca
      241 ccttctacca aaaataactc acctctcatc acatcttggc ctgcacaatc ggctaactta
      301 cccggtaaag cggggccttg agtaattttt ttacgttcaa tttttttgct agcacgtaaa
      361 cgtttattgg cactggcaat cgctaattca gctaattgtt gccccacatc aggatgttga
      421 ttgagccata acgaaaacgc atctttaacc accccttgca caaacaccga ggcttcgcgt
      481 gacgataaac gttctttggt ttgccccgaa aattgcgctt cttgcatttt catcgacaat
      541 acaaacgcca cacgctccca cacatcttca ggggctaact taacattacg gggtaataaa
      601 tttctaaact cacaaaactc acgcatggca tcagttaagc cctgacgcaa accattaacg
      661 tgtgtgccat gttgtacggt aggaattaaa ttaacatagg attcttgaat ggtttcgcca
      721 ccttcgggtt gccacaatac cgcaaactca caagcagcat gttccccctt aaaatcgccg
      781 acaaaaggct cggcaggtaa acattcaaag tctttacaac tgtcaattaa atacgcgcgt
      841 aagccatctt cgtaatacca tgtctcttct tctttggctt gtttatcaat ccatttaatt
      901 tgcaaattag gacataaaac cgctttagcc ttgagtaagt gttttagtgc tttggtatta
      961 aatttaggat tatcaaaata ttgtgggtct gaccaaaaac gcacactcgt gcctgtttga
     1021 cgtttgggca cagacccaat cacctctaat tctttcgatt tattgccatt ggcaaaactg
     1081 atggcatatt cttgaccctc tcttttaact ttaacttgta cttggctgga taaggcgttg
     1141 accaccgaaa tacccacacc atgcaaacca cccgaaaact gatagttttt attagaaaac
     1201 ttaccgcccg catggagttt ggtcaaaatt aactccacac ctgacatttt ttcttcaggg
     1261 tgcatatcaa caggcatacc gcggccatcg tctatcactt ctaaagaatt atcttcgtgc
     1321 aaaatcactt gaatttgaca cgcatggcca gccattgcct catcaaccga gttatcaatc
     1381 acttcttggg ctaaatgatt gggacgactg gtatcggtgt acataccagg acggcggcga
     1441 acagggtcta aacccgataa aacttcaata gcatcagcgt tataagtagt cataagtata
     1501 aaaaatcaat aatcgcagga atatggtgtt caaaactatc aaaggcatga ttaccgccct
     1561 cctccaccaa cattggagac aagggatatt ttgccaaagc tagcctatag tctaaagtct
     1621 catcggcggt ttgtaatagt atccataagc gttctggata cttcaacgcg tcaacttcta
     1681 tcgctaataa ttcatcaact tgggcttggg ttaaaacgta acctttgcct gtatggtaat
     1741 tacgttgctc acccaacagt gaccttaata aatcgtaggc tttaactgca ggattaatta
     1801 ataccgcctt attgccataa tgttgattga gccaagtggc ataaaaacca cccaacgaac
     1861 tgcctaataa accagcctgt gggtattgtt gcactaattg gactaataag ttaatggctt
     1921 gctgcggaaa aatatttaaa tcgggagtaa taaactgaat atcaggccga tgttgagcta
     1981 accattgacg caacaaacct gctttatgag attgagggga actattaaaa ccgtgaat
//
LOCUS       DAOQMB010000424         2037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_37413_length_2037_cov_5.541877, whole genome shotgun sequence.
ACCESSION   DAOQMB010000424 DAOQMB010000000
VERSION     DAOQMB010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37413_length_2037_cov_5.541877"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PLY05_10695"
     CDS             complement(<1..875)
                     /locus_tag="PLY05_10695"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004670442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC03807.1"
                     /translation="MAKIQNRQLTAEELAQFGAELDALRARVQADLGEKDAKYIENIY
                     KSVRYTEIMGRGLLFLSFLPPAWLAGTALLGVSKILENMELGHNVMHGQYDWMRDSRF
                     NGKSYEWDIVGTADNWRKTHNFKHHTYTNIKGMDDDVGYGILRLFPEQRWQPFYLAQP
                     IYSVIFAALFQWGVAIQDLELGKYFAGKKSKEQLRAELKPIWAKTKKQLLKDYLLFPA
                     LAGPLFLPVFLGNLAANGIRNFWTFTIIFCGHFTTDVEIFHKSVLENETRGSWYLRQL
                     RGSSNLTGGKLFHIMS"
     gene            complement(888..1931)
                     /locus_tag="PLY05_10700"
     CDS             complement(888..1931)
                     /locus_tag="PLY05_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HNC03808.1"
                     /translation="MLKELLKPFAAEQITDFWAKELGLTWTWHKPIARIVKRQIEAKD
                     TVSLWLKPNRRFLGFNAGQHISLTVQVNGVYLTRSYSLSHAPQKNGLLRITIKQEAQG
                     KVSQYLTQQAQVGDTFEISQAFGEFSLSAKKPNLLLAAGSGITPLMSLLHTATSQTTL
                     LYWAKTRAELSFVAELKQLKKANPLITVHYFLTQEKELLSHEHHGRISEEILTKLVPD
                     LENYHVLACGNSGFVEQVKTLTSQAPSLQVEAFTPLIPQTTDSTETVQVYLSKSQKTL
                     SLPQGQSLLNALEEQGVYPPSGCRMGICHSCSCQKQSGTTQDLQNGEINQEPNSAVRL
                     CVSLPQSDLVLEI"
BASE COUNT          650 a          403 c          337 g          647 t
ORIGIN      
        1 gacataatat gaaataactt accaccagtt aaatttgatg agccgcgtaa ttgacgtaaa
       61 taccaagacc cacgtgtttc attctctaaa acacttttat ggaaaatttc aacatcagtg
      121 gtaaagtgac cacagaaaat aatggtaaat gtccaaaaat tgcgaatgcc attagccgcc
      181 aaattaccta aaaacacagg taaaaataaa ggacctgcta aagccggaaa taataaataa
      241 tctttaagca attgtttttt ggtttttgcc caaataggtt ttaattccgc acgtaactgc
      301 tcttttgact ttttacctgc aaaatattta cctagctcca agtcttggat ggcaacgccc
      361 cactgaaaca acgcagcaaa aattaccgaa taaattggct gagccaaata aaatggctgc
      421 caacgctgct ctgggaataa acgtaaaata ccataaccta catcatcatc catgcctttg
      481 atattggtat aggtatgatg cttaaagtta tgagttttac gccagttgtc agccgtacca
      541 acaatatccc attcataact tttgccatta aaacgtgaat cacgcatcca atcgtattga
      601 ccgtgcatga cgttatggcc taactccata ttttctaaaa ttttagacac acctaataaa
      661 gctgtgcctg ctaaccatgc tggtggtaaa aagcttaaaa acagtaaacc acgccccata
      721 atttcggtat agcgcaccga tttataaata ttttcaatat acttagcatc tttttcgcct
      781 aagtccgctt gaactctagc acgtaaagca tctaattcag caccaaattg agcaagctct
      841 tctgccgtta attggcgatt ttgtatctta gccatttctt aaatctccta aatctctaaa
      901 accaaatcag attgcggaag actaacacat aagcgcactg cactatttgg ctcttgatta
      961 atttcaccat tttgtaaatc ttgagtagtg cctgactgtt tttggcaact acaactatga
     1021 caaattccca tacgacaacc agaaggtgga taaacgccct gttcttctaa agcatttaat
     1081 aatgattgac cttgtggtaa agacaatgtt ttttgtgact tgcttaaata aacctgaaca
     1141 gtctctgtgg aatctgtagt ttgaggaatt aacggtgtaa aagcttctac ttgtaaactt
     1201 ggtgcttgag aagttaaggt ctttacttgc tcaacgaaac ctgaattacc acaagcgagc
     1261 acatggtaat tttctaaatc tggcacaagt tttgtcaaaa tttcttcgct aatgcgacca
     1321 tgatgttcgt ggcttaataa ttctttttct tgcgttaaaa aataatggac ggtaattagt
     1381 ggattagctt ttttgagttg ttttaattcg gcaacaaaac ttaattcagc acgtgttttt
     1441 gcccaatata ataacgttgt ttggctggtg gcggtgtgta ataaactcat caatggtgta
     1501 ataccactac ctgcagctaa taataaatta ggctttttgg cacttaaact aaactcacca
     1561 aatgcttggc taatttcaaa agtatcgccc acttgtgctt gttgagttaa atattgactc
     1621 actttgcctt gggcttcttg tttaatggta attctaagca agccattttt ttgcggtgca
     1681 tgactcaagc tatatgagcg agtcaaatac acaccattca cttgtacagt cagactaatg
     1741 tgttggcctg cattaaatcc taaaaaacga cgatttggct ttaaccaaag actcaccgta
     1801 tctttggctt caatttgacg tttaacaata cgagctattg gcttatgcca tgtccatgtt
     1861 aaacctaatt ctttcgccca aaagtctgta atttgttcag cagcaaaagg ttttaacagc
     1921 tcttttaaca tgatgatcga caccaaatga ttgatatgtg tagatcataa tcaattaata
     1981 tacatttgta tatacaaatg tatattaata ttattacagt tacaaaagat aattatt
//
LOCUS       DAOQMB010000425         2011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_38348_length_2011_cov_9.655930, whole genome shotgun sequence.
ACCESSION   DAOQMB010000425 DAOQMB010000000
VERSION     DAOQMB010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38348_length_2011_cov_9.655930"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PLY05_10705"
     CDS             <1..1599
                     /locus_tag="PLY05_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC03809.1"
                     /translation="DTQAYATIIELFKDACVKYANKPAFSNLGHTLTFADVDKYSASF
                     AAWLQRNTDLKVGDRIAIQLPNMLQYPIAVFGALRAGLVVVNTNPLYSLRELENQFKD
                     SDCKAVVTLESLARNIQEVLPQTNIRHLIISQAGDLLPLTKRLFINHIVKYLKHKIPR
                     YQLPNAVRFADILKTPPHKFKEVTLSANNIAVLQYTGGTTGIAKGAVLTHSSITSNML
                     QTFAFLSTSLQVGRETMVCPLPLYHVFAFGLSCMGMILGIHTVLITDPRQTNDLINEL
                     KKYRFTSIMGINSLFVSLLNHPDIKTVDFSSLKLTIAGGSLIQPHVAEHWQQVTGCKI
                     CEGYGMTEASPVIAMNPPLAIQTGTIGLPVPNTSIRIVGDDNQDCPLGECGELWVKGP
                     QVMKEYWRHAQATADTITFDGWLKTGDIAQIQADGYLRIVDRKKDIITIGGFNVYPNE
                     IEAVVGQHPAIIECAAVGMIDEQGNEAIKLFIVRKDAQLTENQLQDYLLGQLATYKLP
                     QQIVFVESLPKSSIGKVLRRELKV"
     gene            complement(1596..1832)
                     /locus_tag="PLY05_10710"
     CDS             complement(1596..1832)
                     /locus_tag="PLY05_10710"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNC03810.1"
                     /translation="MADNQSNYPIAIPIIDVDHCTGCGRCVAACPPHVLWLESEKANG
                     WGKKTAVLHTPQECTACAKCYVVCPFDAITMVRT"
     gene            complement(1842..>2011)
                     /locus_tag="PLY05_10715"
     CDS             complement(1842..>2011)
                     /locus_tag="PLY05_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HNC03811.1"
                     /translation="YGWQELARFININCFKNEPSIKSSLVFLRRTPWARHEVEQFYLK
                     YGHEDWLKDQL"
BASE COUNT          647 a          335 c          391 g          638 t
ORIGIN      
        1 gatacacaag cgtatgcaac aattatcgag ttatttaaag atgcttgtgt taaatatgcc
       61 aataaaccag cattctccaa cttaggacat accctaactt ttgctgatgt tgataaatat
      121 agtgcttctt ttgccgcatg gttacaacgt aataccgatc taaaagtagg ggatcgtatt
      181 gcgattcagt tgcctaatat gctgcaatat cctattgcgg tatttggtgc attacgcgcg
      241 ggattagtgg tggttaatac caatccttta tattctttgc gtgaattaga aaatcaattt
      301 aaagattctg actgtaaagc tgttgtgact ttagaaagtc ttgccagaaa tattcaagaa
      361 gtattaccac aaaccaatat tcgccattta attattagcc aagcaggtga tttattacca
      421 ctcaccaaac gtttatttat taatcatatt gttaaatatt taaaacacaa aattccacgt
      481 tatcaattac ccaatgcggt acgttttgct gatatattaa aaacgccacc acataaattt
      541 aaagaggtga cattatcagc taataatatt gctgttttac aatatacggg tggaacaaca
      601 ggtattgcta aaggagcagt attaacacat agtagtatta ccagtaatat gctgcaaact
      661 tttgcttttt tatcgacaag tttacaagta gggcgagaga cgatggtttg tcctttgcct
      721 ttatatcatg tctttgcgtt tggtttgtct tgtatgggga tgattttggg tatacataca
      781 gtattgatta ctgacccaag acaaaccaat gatttaatta atgagctaaa aaaatatcgt
      841 tttacgagca taatgggaat taactcttta tttgtgtctt tattaaatca tcctgacatt
      901 aaaaccgttg atttttcgtc attaaaacta accattgcag gcggttcact cattcaacct
      961 catgttgcag aacattggca acaagtgacg ggatgtaaaa tatgtgaagg ttatggcatg
     1021 actgaagcat caccagtgat agcaatgaat ccgcctttag ccattcaaac gggaacgatt
     1081 ggtttacctg ttccaaatac atcaataagg atcgttggtg atgataatca agattgccct
     1141 ttgggtgaat gtggtgagct atgggttaaa ggcccacaag tgatgaagga gtattggcgt
     1201 catgctcaag caacggctga taccattact tttgatggtt ggttaaagac aggtgatatt
     1261 gctcaaattc aagcagatgg ttatttacgt attgttgatc gcaaaaaaga tattattact
     1321 ataggaggat ttaatgttta tcctaatgaa attgaagcag ttgtggggca acatccggcc
     1381 attatagaat gtgcggctgt agggatgatt gatgaacaag gtaatgaagc aattaaatta
     1441 tttatagttc gtaaagatgc tcaattaacc gaaaatcaat tacaagatta tttattaggt
     1501 caattagcaa cttataaatt accacagcaa attgtttttg tggaaagttt accgaagtct
     1561 tctattggta aagttttaag aagggaatta aaggtttagg tgcgaaccat ggtaatggca
     1621 tcaaaaggac aaacaacata acatttggca caggctgtac attcttgggg ggtatgtaaa
     1681 actgcagttt ttttgcccca accattagct ttttcggatt ctaaccataa tacatggggc
     1741 ggacaggctg cgacacatcg tccacaacct gtacaatggt caacatcaat aataggaatg
     1801 gcaattggat aatttgattg attatcagcc ataatatgaa tttacagttg atcttttaac
     1861 cagtcttcgt ggccatattt tagataaaat tgttcaactt cgtgtcttgc ccaaggagta
     1921 cgacgcaaaa atactaaact cgacttgatg ctaggctcat tcttaaaaca attaatgtta
     1981 ataaaacgag ccagttcttg ccaaccgtat a
//
LOCUS       DAOQMB010000426         2009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_38428_length_2009_cov_16.370522, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000426 DAOQMB010000000
VERSION     DAOQMB010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38428_length_2009_cov_16.370522"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLY05_10720"
     CDS             <1..904
                     /locus_tag="PLY05_10720"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03812.1"
                     /translation="IKRERETILLSDGDHLFLDFHYPSQETANSPLVLVIHGLSGSSD
                     SHYVIGLQKSLATQGIPSVAMNCRGSIQANHAVRAYHAGASDDVRDVFTYLTEHQTRP
                     VIIVGYSLGGSMTLKTLSELGKHPRLLAGVAVSTPLELAPCANRLDAGISIIYRQYLL
                     NGLHELWQKKYQHFLAQDDTVALQRLDNVLQHAPFKSFWQFDDLLMAPLHGFANVDDY
                     YQRCRPNQFLSKIQVPTLVIHALDDPFMSVDVIPAEHALSPQIRFELAKHGGHVGFIG
                     GSLQQPEYYLESRIPAFINSILSP"
     gene            complement(956..1046)
                     /locus_tag="PLY05_10725"
     tRNA            complement(956..1046)
                     /locus_tag="PLY05_10725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1010..1012),aa:Ser,seq:gga)
     gene            1180..>2009
                     /locus_tag="PLY05_10730"
     CDS             1180..>2009
                     /locus_tag="PLY05_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004678880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HNC03813.1"
                     /translation="MSDAHSQQTPLHLLTLGALGVVFGDIGTSPLYALKECFHASHGL
                     AITHDNVLGLLSTIFWAITLVVSIKYIFFIMRADNNGEGGIMALLALSLRNKTISNTQ
                     RLFLVSIGLFGAALFFGDGIITPAISVLSAVEGLKLVTPVFDPYVIPITIGVLISLFM
                     IQRHGTGSVGKLFGPVMLLWFSTLAILGISNISQYPAVLHLVNPYWSIQFILHHPTIA
                     FITLGAVVLTITGGEALYADMGHFGRKPINYAWFGLVFPALILNYCGQGALLLTHPEA
                     V"
BASE COUNT          516 a          390 c          405 g          698 t
ORIGIN      
        1 cattaaacgt gagcgcgaaa ccattttatt aagtgatggc gatcatttgt ttttagactt
       61 tcattatcct agccaagaaa cagccaattc gcccttagtg ttagtaattc acggcttaag
      121 tggttccagt gactcgcatt atgtcattgg tctacaaaag agcttagcca ctcaaggtat
      181 tcccagtgta gccatgaatt gtcgcggttc tattcaagcc aatcatgcgg tacgcgctta
      241 tcatgcaggt gcgagtgatg atgtgcgtga tgtgtttacc tatttaaccg aacatcaaac
      301 acgtcctgtg attattgttg gctactcttt gggtggcagc atgaccttaa aaacgctgtc
      361 tgaattaggt aaacatccac gtttattggc aggtgttgcc gtttctaccc ccctagagct
      421 tgcgccatgt gctaatcgtt tagatgcagg gatttcgatt atttaccgcc aatatttgtt
      481 aaatggttta cacgagttat ggcagaagaa atatcaacat tttttagcac aagatgatac
      541 ggtggcttta cagcgtttag acaatgtcct tcaacatgcg ccttttaaat ccttttggca
      601 atttgacgac ttattaatgg caccattaca tggttttgcg aatgttgatg attattatca
      661 acgctgtcgt cccaatcagt ttttaagcaa aattcaagtg ccaacgttgg tgattcatgc
      721 gttagatgat ccgtttatgt cagtggatgt tattcctgcc gaacatgcct tatcaccgca
      781 aattcgtttt gaacttgcca aacatggcgg tcatgtgggg tttattggtg gtagcttaca
      841 acagcccgaa tattatttag aaagtcgtat tcctgctttt attaatagta tcctcagtcc
      901 atgatttttt tatttgtcta aaaacaaaaa accgcgacaa tgcgcggttt taatctggcg
      961 gtgagagagg gattcgaacc ctcgatacgc tattaacgta tacacgcttt ccaggcgtgc
     1021 gccttcaacc actcggccat ctcaccatat ttagtcttta tgactaacaa ggcgcgcaaa
     1081 tatatactaa tttattagaa aaatcaaaag tattttttat tttttgctca gcggctaatg
     1141 agtgatacac tatagcttac cgtaggctga gattttttta tgagtgatgc gcatagtcaa
     1201 caaacgccct tgcatttatt aacgctaggt gcattaggtg tcgtatttgg agatattgga
     1261 acaagtccgt tatatgcctt aaaagaatgt tttcacgcta gtcatggtct tgcaattact
     1321 catgataatg ttctgggttt gttatctact attttttggg caatcacttt agttgtgtcc
     1381 atcaagtata tcttttttat tatgcgtgca gacaacaatg gtgaaggcgg tattatggcg
     1441 ttacttgcct taagcctgcg taataaaacc attagcaata cccaacgact atttttagtg
     1501 agcatcggtt tatttggtgc agcactcttt tttggtgatg gcattatcac cccagccatt
     1561 tcagttttat cagccgtaga aggcctcaaa ttagtcactc ccgtttttga tccttatgtt
     1621 attccaatta cgattggcgt attaatttca ttatttatga ttcaacgaca tggcacaggc
     1681 tctgttggta agctatttgg ccctgtgatg ctattgtggt ttagtacatt ggcaattttg
     1741 ggcatctcca atatttctca ataccctgct gttttacact tagtcaatcc ttattggtca
     1801 attcagttta ttttgcatca tccaacgatt gcctttatca ctttaggtgc tgttgtctta
     1861 accattacgg gtggtgaagc tttatatgca gatatggggc attttggtcg aaaacctatt
     1921 aattatgcgt ggtttggttt agtttttcct gcattaattt taaattattg tggacaaggt
     1981 gcattattat taacgcatcc agaagctgt
//
LOCUS       DAOQMB010000427         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39210_length_1987_cov_19.613354, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000427 DAOQMB010000000
VERSION     DAOQMB010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39210_length_1987_cov_19.613354"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1830)
                     /gene="glmS"
                     /locus_tag="PLY05_10735"
     CDS             complement(1..1830)
                     /gene="glmS"
                     /locus_tag="PLY05_10735"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNC03814.1"
                     /translation="MCGIVGAVAERNVAAILIEGLKRLEYRGYDSAGLAVIHNSSVVR
                     ERRSGKVRELEIAAEAAQVNGHIGIAHTRWATHGAPTQDNAHPHVSGDIAVVHNGIIE
                     NYEELRHELTTQGYVFVSQTDTEVVAHLVHRELQHTHDLFAAVKKSVTYLEGAYALGI
                     IRASEPARLIAVRKGSPLVIGLGIGENFIGSDQLALLPVTNRFLYLNEGDMAEVYREQ
                     VKVYNEDGQPIQHPITELDAQLHNSDKGGYRHYMLKEIYEQPQAIQRTLDGRVVDGLV
                     NPQAFGVKAPDILAKVKHIQIIACGTSYHAGYVARYWFEALAGVSCNVEVASEYRYRE
                     TVLPENTLLVTISQSGETADTLAALREAQKRGILASLCICNVPTSTLVRESDLSFITH
                     AGPEIGVASTKAFTTQLVALMLLVLNVGHVRGRLNQETEAKIVRQLQNLPVVLESVLG
                     LDKAIERMAIAFGEKHHTLFLGRGAQYPVAMEGALKLKEISYIHAEAYAAGELKHGPL
                     ALVDKDMPVVVVAPNNDMIDKLKSNLQEVRARGGQLFVFADHHAQIRPEEGMTVLGIP
                     SVADEVAPIVYTIPLQLLSYHVAVIKGTDVDQPRNLAKSVTVE"
BASE COUNT          599 a          489 c          362 g          537 t
ORIGIN      
        1 ctactccacc gttacactct tcgccaaatt acgcggttgg tcaacatctg tgcctttaat
       61 aactgccaca tgatacgaca ataactgcaa cggaatagta tagacaatcg gtgcaacctc
      121 atcagccaca ctcggaatcc ccaacacagt cataccttct tcgggacgaa tttgtgcatg
      181 atgatcggca aagacaaata attgaccacc acgcgcacgc acttcttgta agtttgattt
      241 taacttatca atcatgtcgt tattaggtgc aaccaccacc acaggcatat ctttatcaac
      301 gagagctaaa ggcccatgtt ttaactcacc tgcggcatag gcttcggcat gaatataaga
      361 aatctcttta agctttaacg ccccttccat tgccacagga tattgcgcac cacgacctaa
      421 gaataaggta tgatgttttt caccaaaagc aattgccata cgctcaatcg ctttgtctaa
      481 gcccaagaca ctttctaaca caacaggtaa gttttgcaat tggcgaacaa tcttggcttc
      541 ggtttcttgg tttaaacgac cgcgaacatg accgacattt aacaccaaca acatcaatgc
      601 cactaattgt gtggtaaagg ctttggtaga cgccacacca atttctgggc ctgcatgggt
      661 aataaacgat aaatcagact cacgaaccaa ggtggaggtt ggcacattac aaatacacaa
      721 agaagctaaa atgccacgtt tttgtgcttc acgtaaagcc gctaaagtat ctgccgtttc
      781 gcccgattgc gaaatagtga ctaacaaggt attttcgggt aatactgttt cgcggtagcg
      841 atactcgctc gctacttcaa cattacacga gacacctgct aaagcttcaa accaataacg
      901 tgccacataa cctgcgtgat aactggtacc acaagcaata atttgaatat gtttaacctt
      961 agccaaaata tcaggtgctt taacaccaaa agcttgtgga ttgactaatc catcaacgac
     1021 acgaccatct aaagtgcgtt gaatcgcttg tggctgctca taaatttctt tgagcatata
     1081 gtgacgatag ccacctttat cactgttatg taattgtgca tctaattcag taatcggatg
     1141 ttgaataggc tgtccatctt cgttataaac tttgacttgt tcacgatata cttctgccat
     1201 atcaccttcg tttaaatata aaaaacgatt agtgacaggt aacaacgcta attgatcaga
     1261 gccaataaaa ttttcaccta tacctaaacc aatgactaag ggcgaacctt tacgcaccgc
     1321 aattaaacgt gcaggttcag aggcacgaat aatgcctaac gcgtaagcac cttctaaata
     1381 agtcacagat tttttaaccg cagcaaataa atcgtgtgta tgctgtaatt cacgatgcac
     1441 caaatgggca accacctcgg tatcggtttg agaaacaaaa acataacctt gagtggtcaa
     1501 ttcatggcgt aattcttcat aattttcaat aatgccgtta tggacaacgg caatatcacc
     1561 agagacatgc ggatgggcat tatcttgggt tggtgcacca tgtgttgccc aacgagtatg
     1621 agcaatgcca atatgaccat taacctgtgc tgcttcagcc gcaatctcta attcacggac
     1681 tttacctgaa cgacgctcgc gcactacgct actattatga atgaccgcca agcccgcaga
     1741 gtcatatcca cgatactcta aacgttttaa gccttcgatt aaaatggctg ccacattacg
     1801 ttcggcgact gcgcctacaa tcccacacat agtttctctc ctaaactatt agttattttt
     1861 tcttttgggg acgattccat ccctcaatgt tacgctgaat gccacgtgca accgccaaat
     1921 gattagcagg cacatcttta ctaatcgcag aacctgcacc tgttgttgct ccatcaccaa
     1981 tgacaac
//
LOCUS       DAOQMB010000428         1986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39247_length_1986_cov_18.125842, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000428 DAOQMB010000000
VERSION     DAOQMB010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39247_length_1986_cov_18.125842"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..>1986
                     /locus_tag="PLY05_10740"
     CDS             146..>1986
                     /locus_tag="PLY05_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC03815.1"
                     /translation="MIQYQDLTLRRSGRVLFANAAGMIHPSWKVALTGENGAGKSSFF
                     SALLGGLTPDAGTLSRPQSWTVAHMAQEVTALGRTAIDFVLDGDKEWRQINDALETEQ
                     DDNKLADLYSEFDTIDGYTAPARAAQLLAGLGFSETAQQKTVSEFSGGWRMRLNLAQA
                     LMCRSDLLMLDEPTNHLDLDAILWLEDWLNRYQGTLILISHDRDFLDATVGHILHIEQ
                     QTMTLYTGNYTTFERTRAERLAQHQQAFEKQQSEMAHLQKYIDRFRAQATKARQAQSR
                     IKQLERMVLIAPAHVDSAFSFSFREPAKMGTPLVKLDAVNAGYGDKVIVNNINLNISP
                     DTRIGLLGPNGAGKSTLIKTLVGELPLLAGERVCSEHLVLGYFAQHQVDHLDMQASPL
                     LALTRIAPRTSELELRKFLGSFGFNGDRVTTTVENFSGGEKARLALALIVWQRPNVLV
                     LDEPTNHLDLEMRHALSMALQAFTGALIVVSHDRQLLGSCCDEFMLVADGKIDIFGGS
                     LVDYAAWLKEWRAKQDVKVESTATRQEKREERQQVKSTGDNLHILRPLRQQAEKLEAK
                     FNRLTEQQSHLASQLEDMSLYQDNNKDKLKKLLAEKSKVDSELAEVEV"
BASE COUNT          576 a          425 c          445 g          540 t
ORIGIN      
        1 cgacaggaat cgaacctgta attgcccctt aggagggggc cgttatatcc atttaactac
       61 gaggagaaaa cggcggcaat tttacccaaa gtcatgataa accgctacaa tataccacct
      121 cttttgttgt tctagcctca tcaccatgat tcaataccaa gacttaacct tgcgccgtag
      181 tggccgcgta ttatttgcca atgctgctgg catgattcac ccttcatgga aagttgcact
      241 cactggtgaa aatggcgcag gcaaatctag ctttttttcg gctttattgg gtggtttaac
      301 tcccgatgct ggcacattat cacgaccaca aagttggaca gtggcacaca tggcgcaaga
      361 agtcaccgca ttaggtcgta ctgccattga ttttgtcttg gatggtgata aagaatggcg
      421 acaaattaat gatgccctcg aaaccgaaca agacgacaat aaactagccg atttatacag
      481 tgaatttgac accattgacg gttataccgc gcctgctcgc gcagcacaat tattagcagg
      541 attgggcttt agtgaaacgg cacaacaaaa aaccgttagc gagttttcgg gcggttggcg
      601 aatgcggtta aatttggcac aagcattaat gtgccgttct gatttattga tgcttgacga
      661 accgaccaac cacttagact tagatgcgat tttgtggctt gaagattggc tcaatcgtta
      721 tcaaggtaca ttaattttaa tttcccacga tagagacttt ttggatgcca ctgttggtca
      781 tattttgcat atcgaacagc aaaccatgac cctatataca ggcaattaca ccacctttga
      841 gcgcacccgt gctgagcgtt tggcacaaca tcaacaagct tttgaaaaac aacaaagtga
      901 aatggcgcat ttgcaaaaat atatcgaccg tttccgtgcc caagccacta aagctcgcca
      961 agctcaaagt cggattaaac aactcgaacg catggtgtta attgcacctg cccatgttga
     1021 ctctgcattt agctttagct ttagagagcc tgcgaaaatg ggtacaccac tggtaaaact
     1081 agatgcagta aatgcaggtt atggcgataa agtcattgtc aataatatca atctcaatat
     1141 tagccctgat acacgcattg gtcttttagg ccctaatggt gcaggtaaat cgactttgat
     1201 taaaacttta gtcggtgaac tgcctttatt agcaggcgag cgcgtatgta gcgaacattt
     1261 agtgttaggt tattttgcgc agcatcaagt tgaccattta gatatgcaag cttcgccctt
     1321 gttagcccta acccgtattg caccacgtac ctccgaactc gaattgcgta agtttttggg
     1381 tagctttggt tttaatggtg accgtgttac aacaacggtt gagaattttt cgggtggtga
     1441 aaaagcccgt ttggccttag ccttaattgt ttggcaacgt cctaatgtgt tagtgttaga
     1501 cgaaccaacc aaccacttag acctcgaaat gcgccatgcc ttaagtatgg ctttgcaagc
     1561 ctttacgggg gcattgattg tcgtctcgca cgatagacaa ctcttgggca gttgttgtga
     1621 cgaatttatg ttggtggccg atggcaaaat tgatattttt ggtggttcat tagttgatta
     1681 tgccgcatgg ctgaaagagt ggcgtgctaa acaagatgtt aaagtcgaaa gtacagcaac
     1741 tcgccaagaa aaacgcgaag aacgccaaca agtcaaaagc actggtgata atttgcatat
     1801 tttgcgccct ttacgccaac aagccgaaaa gttggaggcc aagtttaatc gtctcaccga
     1861 acagcaaagc catttggcca gccaacttga agatatgagt ctttatcaag ataacaataa
     1921 agataagctc aaaaaattac tggccgaaaa aagtaaagta gatagcgaac tggcagaggt
     1981 agaagt
//
LOCUS       DAOQMB010000429         1985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39292_length_1985_cov_4.602073, whole genome shotgun sequence.
ACCESSION   DAOQMB010000429 DAOQMB010000000
VERSION     DAOQMB010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39292_length_1985_cov_4.602073"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1685
                     /locus_tag="PLY05_10745"
     CDS             <1..1685
                     /locus_tag="PLY05_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HNC03816.1"
                     /translation="RLDTIAQDIVSHFPQRGYLGKGMMVAVDQFTAVRMYEKVKTLWD
                     KEIRQLQSQIYQTKNDLQKQQLKNRLKYMQSVQMAVVISDPSADKARFEQQGLAIQAH
                     IDRLHKLDEHGCDVEDNFKNPEHPLQLVFVCAMWLTGFDAPTVSTLYLDKPMKGHTLM
                     QTIARANRVTSYRIMNDVGQMVEKTNGEIIDYYNVFRNLKAALKDYGQGEDGAESPVQ
                     EKQALFTMLDEAIAQTIAFCQDKNIDLTKMLQQDLGTFKQNALFKDYADILLSKDEYR
                     KSYNVHFNTVAALYEACKPNILATPKPIVAVMQFLQGFVAAMVERQELSGVVNQVADL
                     LDRSVVVDSSHAVNEPKAAYPIIASKKWDLSTVDFDKLKEQFKATKHKHIAIADMRAF
                     LDKKLLEMLKQNSGRMSFAERYQKVIENYNAGATATEQFFEELQQFTQSLQTEEQRHI
                     REGLTEDELEVYDLLLKDKLSKAEEQQVKLAAKDLIIKLIGASPKVLIQDWWKDAQTQ
                     AVVKSLIEDVLDKDLPNSYEPIVFKEKVSHVFDLLKTLAMNDEKWANVPQSA"
BASE COUNT          685 a          354 c          405 g          541 t
ORIGIN      
        1 acaggctcga taccattgct caagatatag tgagtcattt tccacagcgc ggctatttgg
       61 gtaaaggtat gatggtggcg gttgaccaat ttaccgccgt acgtatgtac gaaaaagtca
      121 aaaccttgtg ggacaaagaa attcggcaac tacaaagcca aatttatcaa actaaaaatg
      181 acctccaaaa gcaacaactc aaaaatcgct taaaatatat gcagtcggtg caaatggcgg
      241 tggtgattag tgaccccagt gccgacaaag cgcgttttga acaacaaggt ttggctattc
      301 aagctcatat cgaccgttta cataaactcg atgaacacgg ctgcgatgtg gaagataact
      361 ttaaaaatcc agagcatcct ttacagttag tgtttgtctg tgccatgtgg ttaacaggtt
      421 ttgatgcgcc taccgtctca accttgtatt tagataaacc catgaaaggc cacaccttaa
      481 tgcaaaccat tgcgcgtgcc aatcgggtga cttcctatcg tattatgaat gatgtagggc
      541 agatggtaga aaaaacaaat ggtgagatta ttgattatta taatgttttt agaaacttaa
      601 aagccgctct taaagattat ggacaaggtg aagacggtgc agaatcgcct gtccaagaaa
      661 aacaagccct gtttactatg ctcgatgaag cgattgctca aacaatagcg ttttgccaag
      721 acaaaaacat tgatttaacc aagatgctcc aacaagattt aggcacattt aaacaaaatg
      781 ccttgtttaa agattatgct gatattttgt tatctaaaga cgaatatcgc aaaagttata
      841 acgtgcattt taatacggtt gctgccttat acgaggcgtg taaacctaat attttggcaa
      901 cgcctaaacc cattgtcgca gttatgcagt ttttgcaagg ttttgtcgcg gcaatggttg
      961 agcggcaaga attgtcgggc gtggttaatc aggtggcaga tttattagac cgtagtgtcg
     1021 tggttgatag tagccatgcg gttaatgagc ctaaggccgc gtatccgata attgctagta
     1081 aaaaatggga tttaagcacc gttgattttg acaaactcaa agagcaattt aaggcaacca
     1141 aacataaaca tattgccatt gccgatatgc gtgccttttt agataaaaaa ttgcttgaaa
     1201 tgctcaaaca aaatagtggt cggatgagtt ttgccgagcg ataccaaaaa gtcatcgaaa
     1261 actataatgc aggtgcaacc gcgaccgaac aattttttga agagttacaa cagtttaccc
     1321 aaagtttaca aacagaagag caacgtcata ttcgtgaagg gcttacagaa gacgagttag
     1381 aagtttatga tttactttta aaagacaaac tcagcaaagc cgaagagcaa caagtaaaat
     1441 tagccgctaa agatttaatc attaaattga ttggggcaag ccccaaagtg ctaattcaag
     1501 attggtggaa agacgcacaa acccaagcgg tggttaaatc tttgattgaa gacgtgttag
     1561 ataaagactt gcctaatagt tatgaaccca ttgtttttaa agaaaaagtc agtcatgtgt
     1621 ttgatttact aaaaacactg gcgatgaatg atgaaaaatg ggcaaatgtg cctcagtcag
     1681 cctaaaatga gttttttaag agtaaaaata cggtgactac attaaacaat ctactcagta
     1741 cacaacaatt aatcaagctt atgaagaggc atttaggttg atatgcaaaa taggcgatat
     1801 tttaaaacaa caaaaacaaa gcttagcatt tagtgattat gtggcagagt taagtgtcaa
     1861 ctacaagtta aaacgtaatt ttatggcgat gctgaaaaaa ataccgcccc actaaaaaat
     1921 aaaaatttat tttaaccctt ttttaaaatc ttggcgttta cttaacaaaa taaccccatc
     1981 ctgta
//
LOCUS       DAOQMB010000430         1983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39360_length_1983_cov_20.013485, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000430 DAOQMB010000000
VERSION     DAOQMB010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39360_length_1983_cov_20.013485"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="PLY05_10750"
     CDS             complement(<1..1380)
                     /locus_tag="PLY05_10750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNC03817.1"
                     /translation="MTHNTAPTISGYTIHEVLGAGGMATVYLATQHSLQRQVAIKVLE
                     ANQEAQLYQRFINEAHIVASLHHPAIISIYDVQQLADGRCYLVMELIKSGDLSQYKGQ
                     KFEPKRALAIIKQIAQGLAVVHHQGLIHRDIKPANILFRDHEHIVITDFGIAKNTQIN
                     QELTQHGVVVGSPSYSSPEQAQCQEIDHRSDLYSVGVIFVEMLLGYNPYRGENYTQTV
                     VNHVQMDIPTLPQELSPYQAIVNKLLAKQPDQRFSDSQSLVNAIDGLLTVSIPIYSPT
                     VSTQKKSVSKQKVIVGVVAILLFLLALITAPMVKKYWQVRQLLQQAEQRLQQKKWLSP
                     ANDSAQFFYQQALNLDADNQQAKTGLTTAKNQQIAYWLTQAETKFASGDLSTPKDDNA
                     VFYFQQIQTIDAKQPQAQAGLQRIAQVYLGLAQEDYQQKQFEQALNAIKLGLLAQPNH
                     SELLKLAAEH"
     gene            1484..1888
                     /locus_tag="PLY05_10755"
     CDS             1484..1888
                     /locus_tag="PLY05_10755"
                     /inference="COORDINATES: protein motif:HMM:NF020652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HNC03818.1"
                     /translation="MRLLAYLITSCLISFQAYAAENANSQLQQLASQAKAENSAFTAF
                     NAQRGQQFFTSTHGSDWSCASCHTKNPLQTGKHDVTGKIIQPMAPSANPARFTDVAKT
                     QKWFKRNCKDVVGRECTAQEKGDVLTWLISLK"
BASE COUNT          586 a          380 c          402 g          615 t
ORIGIN      
        1 atgttcggca gcgagtttta ataattctga atgattgggc tgagccaata agccaagttt
       61 aatagcgttg agtgcctgtt caaattgttt ctgctgataa tcttcctgtg ctaagccaag
      121 ataaacttgg gcaatacgtt gcagaccagc ttgtgcttgt ggctgtttag catcaatcgt
      181 ctgtatttgc tgaaagtaaa ataccgcatt gtcgtctttg ggagtcgata aatcgccaga
      241 agcaaatttg gtttcggctt gtgttagcca ataggcgatt tgttgatttt tggcagtcgt
      301 taaacctgtt ttggcttgtt gattatcggc atctaaattt aaggcttgtt gataaaaaaa
      361 ctgtgcactg tcattggcag ggctgagcca tttcttttgt tgtaaacgct gttctgcctg
      421 ttgcaataat tgacgaactt gccaatattt tttgaccatt ggcgcagtaa taagtgctaa
      481 taaaaacaat aaaatagcaa caacgccgac aataactttt tgcttactaa cagacttttt
      541 ttgtgtgcta acagtaggtg aataaattgg gattgatacg gttaataaac catcaatcgc
      601 attaactagg ctttggctat cgctaaaacg ttgatcgggt tgtttggcta ataatttatt
      661 gacgatggct tgatagggac tcagttcttg aggcaaggtt ggaatatcca tttgcacatg
      721 attaaccacc gtttgggtat aattttcacc acgataaggg ttatagccca ataacatttc
      781 aacaaaaatc acgccaacac tatataaatc actacgatgg tcaatttctt ggcattgtgc
      841 ttgttcggga ctactatacg atggactacc aaccaccaca ccatgttggg ttaattcttg
      901 attaatttga gtatttttag caataccaaa atcggtaatg acaatatgtt cgtggtctct
      961 aaacaaaata ttcgcaggtt taatatctcg gtgaatcaaa ccttgatgat gaaccaccgc
     1021 taaaccttga gcaatttgtt taataatcgc caatgcgcgt tttggttcaa acttttggcc
     1081 tttatattga ctcagatcac cgcttttaat taactccatt accaaataac aacgaccatc
     1141 ggctaattgc tgcacatcat aaatgctaat aatcgcaggg tggtgcaaag aggccacaat
     1201 atgcgcctca ttaataaaac gttggtacag ttgagcttct tgattagcct ctaaaacttt
     1261 aatcgctact tggcgttgca aagaatgttg tgtggctaaa tagacggtcg ccataccacc
     1321 cgcccctaag acttcatgga tggtgtagcc tgaaatagta ggagctgtat tgtgggtcat
     1381 tattgttaaa aacctatcag taaaataatc tgcatcgaat taagctttat ttaagtttaa
     1441 aattttatag tcattttaaa caagccatga ttggggaaga acaatgcgtt tattagccta
     1501 tttgatcaca agttgtctaa ttagttttca agcgtatgct gccgaaaacg ccaatagtca
     1561 gttacaacaa cttgctagtc aagcgaaagc tgaaaactca gcatttaccg cgtttaatgc
     1621 ccaacgaggt caacagtttt ttacatcaac gcatggtagt gattggagtt gtgccagttg
     1681 tcacaccaaa aatccactgc aaacgggcaa gcatgatgtc acaggaaaaa taattcagcc
     1741 aatggcacca tcagccaatc ccgctcgttt tactgatgtc gctaaaacac aaaaatggtt
     1801 taagcgtaat tgcaaagatg ttgtcggtcg tgagtgtacc gcccaagaaa aaggcgatgt
     1861 gttaacatgg ttaatatcat taaagtagtt aaatcctccc cagccctcct ttaagaaagg
     1921 agggagttcc ccctttttag gggtaatgct ggtcagttaa gcatttcttg ttgatttcga
     1981 ctc
//
LOCUS       DAOQMB010000431         1982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39402_length_1982_cov_13.417748, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000431 DAOQMB010000000
VERSION     DAOQMB010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39402_length_1982_cov_13.417748"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1982
                     /gene="glnD"
                     /locus_tag="PLY05_10760"
     CDS             <1..>1982
                     /gene="glnD"
                     /locus_tag="PLY05_10760"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HNC03819.1"
                     /translation="YRQLLKDCQQQLWERFNNGEDVRLLVQARAETVDLVLQHVWRRF
                     DLEQETEVALLAVGGYGRGELHPYSDVDVLVLHRHNQLSQELEQKISQFITLLWDLGL
                     DIGSSVRSLETCIDKVNEDVTIATTFLENRTLCGDDGLRQNLLELIAPLWSNAVFFEA
                     KRDEQIARHRKHNDTEYNLEPDLKNAPGGLRDIQTICWVTKRHFQSSNLYDLVRNGFL
                     TEFEYKKLYEGETFLWRVRFALHYIAKRNENKLLFDYQRTLALQFGYSDNNANRAVEQ
                     FMKQYYRVAMHLSMLNEMLLQYFDEAILKADEPANIEVLSEDFQLVNNQLEVRHHQVF
                     ARNPSALMELFAVLSDNPDIEGVRASTIRLIMVEARKINDDFRNNPQNKAYFIEILRS
                     SRYLFSTLRRMKRYGVLGQYLPAFGAIIGQMQYDLFHIYTVDAHTLLVIKNMRRFRYA
                     ETREQFPVVAEVAQNLNKPELLYLAGLFHDIGKGRGGDHSELGSVDAIEFCLSHGLSQ
                     RSANLVAWLTKHHLLMSMTAQKQDLTDPEVIHNFAKQVGDIAHLDYLYALTVADICAT
                     NPKLWNSWRASLLRQLYQQTRRALRRGLNNPIDYEELIAETRITAMEELLAEGFDEQA
                     ITAIWQNLGDDYFLRETAKDVAWHTREILQHGNSTQP"
BASE COUNT          565 a          380 c          418 g          619 t
ORIGIN      
        1 tatcgtcagt tattaaaaga ttgtcaacaa caactttggg aacgttttaa taacggtgaa
       61 gatgtgcgtt tattggttca ggctcgcgca gaaactgttg atttagtgtt gcaacacgtt
      121 tggcgacgct ttgatttgga gcaagaaaca gaggttgcct tattagccgt tggtggttat
      181 ggtcgtggtg agttacaccc ctattctgat gttgatgttt tagtgttaca tcgtcacaac
      241 caactcagcc aagaacttga gcaaaaaatt agtcaattta ttaccttatt atgggatttg
      301 ggtttagaca ttggttcgag cgtccgttcc ttagaaacgt gcattgataa agtcaatgaa
      361 gatgtcacca tcgccactac ttttttagaa aatcgtacct tgtgtggtga tgatggttta
      421 cggcaaaact tgttagagtt aattgcgcct ttgtggtcta atgccgtctt ttttgaagct
      481 aaacgtgatg agcaaattgc gcgtcaccgc aaacataatg ataccgaata taacctcgaa
      541 cctgacctaa aaaatgctcc cggtggtttg cgtgatattc aaaccatttg ttgggtgacc
      601 aaacgccact tccaaagctc aaacttatat gacttagtta gaaatggctt tttaaccgaa
      661 tttgaataca aaaaactgta cgaaggtgaa acctttttat ggcgagtacg ttttgctttg
      721 cactacattg ccaaacgtaa tgaaaacaaa ttgttatttg attatcaacg caccttagca
      781 ttgcagtttg gttatagcga caataatgcc aatcgtgcgg ttgagcagtt tatgaaacag
      841 tattatcgcg ttgccatgca cttgtcgatg ctcaacgaaa tgctgttgca atactttgat
      901 gaagcgattc taaaagcaga tgagcctgcg aatattgaag tattgtctga agacttccaa
      961 ctggtcaata atcaacttga agttcgccac catcaagtgt ttgcccgcaa ccctagtgcg
     1021 ttaatggagt tatttgcagt tttatctgac aacccagata ttgagggggt tcgtgctagc
     1081 accattcgtt tgattatggt tgaagctcgt aaaatcaatg acgacttccg taataatccg
     1141 caaaacaaag cctattttat tgaaatttta cgttcatcac gttatttgtt ttcgacatta
     1201 agacgcatga aacgttatgg ggttttaggc caatatttac ctgcctttgg tgcgattatt
     1261 ggccaaatgc aatatgactt atttcatatt tatacggttg atgcccatac cctgttggtc
     1321 attaaaaata tgcgccgttt tagatatgcc gaaacacgcg agcaatttcc tgtggttgct
     1381 gaagttgccc aaaatcttaa taaaccagaa ttattgtact tagctggttt attccatgat
     1441 attggcaaag gtcgtggtgg agaccactca gaacttggtt cagttgatgc cattgaattc
     1501 tgtttaagtc atggtttatc gcaacgtagt gccaatttag ttgcatggtt aaccaaacat
     1561 catttattga tgtcgatgac cgcccaaaaa caagatttaa ctgaccccga agtcattcat
     1621 aactttgcca aacaggtggg tgatattgct cacttagatt atttatatgc tttaacagtc
     1681 gcggatattt gtgcaactaa tccaaagcta tggaactctt ggcgtgcgtc cttattaaga
     1741 cagctttatc agcaaacccg tcgtgcctta cgtcgtggtt taaacaaccc cattgattat
     1801 gaagaattga ttgccgaaac acgtattacc gctatggaag agttattggc cgaaggtttt
     1861 gatgagcaag cgattaccgc tatttggcaa aacttaggtg atgattattt cttgcgtgaa
     1921 acggccaaag atgtggcatg gcatacccgc gaaattttgc aacatggcaa cagcacacaa
     1981 cc
//
LOCUS       DAOQMB010000432         1976 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39652_length_1976_cov_12.559604, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000432 DAOQMB010000000
VERSION     DAOQMB010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39652_length_1976_cov_12.559604"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="PLY05_10765"
     CDS             complement(<1..569)
                     /locus_tag="PLY05_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03820.1"
                     /translation="MENLTIDTQLVWMTCGIDAGYQILLAPPEGSKSWVDLLEGAFPN
                     FELGGSNLKTVEINYDGNKALALAFFDPELCDASSRPIKHFMLFLGLNESVIKRAPSL
                     LIEPLLQQLAPAFREICRASRYSSEQDIMKLAQNKQTERIIHLEFKSKLATVAAVVSK
                     KVEASKAAPNRATAAHGQDTIEEHEALSMP"
     gene            complement(579..1718)
                     /locus_tag="PLY05_10770"
     CDS             complement(579..1718)
                     /locus_tag="PLY05_10770"
                     /inference="COORDINATES: protein motif:HMM:NF040097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03821.1"
                     /translation="MAKQDKPLQTLKIAFLGGRGCGKTTLLASYLGHMASSRWQNEHH
                     YYLSTPDSSDSKRLNELFQGLCNGFFPEATIKRASAYRFQMHIQDCEGVPLEIQWLDY
                     PGEWWEREPVDEKEKKQRDDCLQRMVNSHVCFLVIDGAQFQRHGETYLRAHLAHMTNE
                     IANLLRQTGHQKKKNNPLKDMVWVMALSKADMLESQLTAADFADFVNRYAGDQLDTLR
                     QRLGEAGCPSFGMNYLVFSSVLGVGQRIIDINKTIGLDLIAPLALRTILEKELQNSEK
                     SAVLKRIFAGSADRGALQALFNLLRQILAKWPDRSTRDLGDLLFTKAETWLNGRAQDL
                     QNEMNQYQKQGYSLQVAESVLKLALSKPENRRYFYVPLLRIGASR"
BASE COUNT          619 a          443 c          325 g          589 t
ORIGIN      
        1 ggcattgaga gtgcttcatg ttcctctata gtatcttgtc catgagcagc agtagcccta
       61 ttaggagctg ctttacttgc ctcaaccttt ttactcacaa cagcagcaac cgttgccaat
      121 ttacttttaa actctaaatg aataatccgt tcagtttgtt tattttgtgc aagcttcata
      181 atatcttgct ctgagctata acgtgaagcg cgacaaatct ctctaaaagc aggtgctaat
      241 tgttgcaata aaggctcaat taataatgaa ggtgcgcgtt taatgacact ctcatttaat
      301 cctaaaaaca acataaaatg tttaattggg cgtgagcttg catcacacaa ctcaggatca
      361 aaaaacgcta aagccaacgc tttattacca tcataattaa tttcaacggt ttttaaattt
      421 gaaccgccca actcaaaatt aggaaaagcc ccttctaata aatcaaccca agatttagaa
      481 ccttcaggtg gtgctaataa aatttgataa cccgcatcta tgccacaagt catccatact
      541 aattgcgtgt ctatggttaa attttccatg ccctacctct agcgactcgc cccaatacgt
      601 aataatggca cataaaaata ccgccgattt tcgggcttac ttaaggcgag tttaagcact
      661 gactccgcaa cttgcaagct atatccttgt ttttgatact gattcatctc attttgcaaa
      721 tcttgagcac gaccattcaa ccacgtttct gccttggtaa acaataaatc acctaaatcg
      781 cgtgttgagc ggtctggcca tttagccaaa atttgccgca ataaattaaa caatgcctgt
      841 aaagccccac ggtcggcact gcctgcaaaa atacgtttta acacggctga cttttcactg
      901 ttttgtagct ctttttctaa aatggtacgc aaggccaaag gtgcaattaa atctaaacca
      961 atggttttat taatatcaat aatccgttga ccaacaccta ataccgacga aaagactaaa
     1021 taattcatgc caaaagaggg acaacctgct tctcctaaac gttggcgtaa agtgtctaat
     1081 tgatcgcctg cataacgatt aacaaaatcg gcaaaatcgg cggcggttaa ttggctttcc
     1141 aacatatccg ccttacttaa agccataacc caaaccatat cttttaaggg attatttttt
     1201 ttcttttgat gtcctgtttg ccgcaacaag ttggcaattt cattggtcat atgggctaaa
     1261 tgagcgcgta aataggtttc gccatgacgt tgaaactgcg caccatcaat caccaaaaaa
     1321 catacgtgac tattaaccat acgctgtaaa caatcatctc gttgtttttt ctctttttca
     1381 tcaacgggtt cacgctccca ccactcacca ggataatcta accattgaat ctctaaaggc
     1441 acaccttcac aatcttgaat atgcatttga aaacgataag ctgaggcacg tttaatggtc
     1501 gcttcgggaa aaaaaccatt acataagccc tgaaatagct catttaaacg cttagagtcg
     1561 ctggaatctg gtgtactgag ataataatga tgttcattct gccaacgact actggccata
     1621 tgacctaaat aactggctaa taacgtagtt tttccgcaac cacgtccgcc taaaaaagct
     1681 atttttaatg tttgtaaggg tttatcttgt ttggccatga gtatctaaag cctgagctgt
     1741 aatttcgtag aatttaaaaa atagtaacca attatcaaaa aacgttatca aaatagccgt
     1801 ctttccaagt ctatttttgc gtcaaactcg gctactctgc ctaaatgtta acaacgacgc
     1861 aagcaagttt ttgcaagaat tgtgcaaata tcttacaaat atactctgtg actaacacca
     1921 caaatcagat attaatatgc tcttgacgtg cgccacttgg caaaaataca cttgtt
//
LOCUS       DAOQMB010000433         1974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39723_length_1974_cov_15.831162, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000433 DAOQMB010000000
VERSION     DAOQMB010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39723_length_1974_cov_15.831162"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..>1974)
                     /gene="mrdA"
                     /locus_tag="PLY05_10775"
     CDS             complement(89..>1974)
                     /gene="mrdA"
                     /locus_tag="PLY05_10775"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNC03822.1"
                     /translation="LGFCTLRLKSRPSPTNPDPLKNSALESRIFRNRIWVTAFFIALA
                     FSTLLIRYGYLQILEHQNYKTLADSNRIKLQAIAPPRGYIYDRNGILLADNRPIFTAM
                     VNRQEIDNLDQTIERLTPIFQLTPEDIQRFKNRVKNAPRYESVAIKLNLRESDIARFS
                     EVAFLFKGVNIEVKLARYYPYGELFAHVLGYVGRISDKEAATLNAERYAGTDLIGKTG
                     LEKYYESILQGKAGYQQIEANAHGQVLRLLERTEPTRGNDLVLHLDYGLQKMLSEKLA
                     GKRAAVVAIDPKTGGVLAFVSTPSFDPNPFVAGVPNNLYNFWRDHPDVPLYNRALQGI
                     YPPASTIKPIFGMAGLHYGLVDWNYKIQDRGRYSIPGDWHVFRDWKKSGHGTVDLHRA
                     VEISCDTYFYQLAYKIGVDRFYDWMQNFGFGKPTGIDLAGEKTGVLPSTAWKKRVLKA
                     PWYTGEMISVGIGQGYFTTTPLQLAMATAIMANKGQHLTPHLLKSTTGIYPYQVINQP
                     DGQVPFAGQADNWDKMHQAMKAVVHGRGTAGNLRKDLEGYEIAGKTGTAQVKGIKQGA
                     RYNEKELDERHWDHAWFMGFAPADNPQVAVAVLVENGKHGNSAAGPIAKAVFDYVIHQ
                     MKR"
BASE COUNT          597 a          406 c          388 g          583 t
ORIGIN      
        1 tttataatgg cggccacttt ttgcaagaag accaaggtga acaattggcg gaggtggtag
       61 tcaaattttg tcaaaaaatc aggtctagtt aacgcttcat ctgatgaatg acgtaatcaa
      121 acacggcttt ggcaataggc ccagcggcac tgttgccatg tttgccgttt tctactaaca
      181 cagcaacagc aacttgtgga ttatcagcag gggcaaaccc cataaaccat gcatgatccc
      241 aatgacgttc gtctaattct ttttcgttgt agcgtgcgcc ttgtttaata cctttgactt
      301 gtgcagtgcc tgtttttcct gcgatttcat aaccttctaa atctttgcgt aagttgccag
      361 ccgtgccgcg accatgcaca accgctttca tggcttgatg cattttgtcc caattatctg
      421 cttggccagc aaaaggaact tgaccatcag gttgattaat cacttgataa ggatatatac
      481 ctgtggttga ttttaataaa tgtggggtta aatgttggcc tttattggcc ataatagcgg
      541 tagccatcgc taattgtaat ggtgtggtgg taaaatagcc ttggccaata cccaccgaaa
      601 tcatctcacc tgtgtaccaa ggcgctttaa gaacacgttt tttccatgcc gtcgaaggaa
      661 gaacccctgt tttttcacct gctaaatcaa tacccgttgg tttgccaaaa ccaaagtttt
      721 gcatccaatc ataaaagcga tcaacaccaa ttttataagc caattgataa aaataagtat
      781 cacaagaaat ttctactgca cgatgtaaat cgactgtgcc atgaccagac tttttccaat
      841 ctctaaatac atgccagtca ccgggaatac tataacgacc tctatcttga attttataat
      901 tccaatcgac taaaccataa tgtaaacctg ccataccaaa aataggttta atggttgagg
      961 cgggcggata aataccttgt aaggcacgat tatataaagg tacgtcagga tggtctcgcc
     1021 aaaaattata taaattatta ggaacacctg cgacaaaagg attgggatca aaacttggtg
     1081 tactgacaaa ggctaaaaca ccacctgttt tggggtcaat ggcaacaaca gcagcacgtt
     1141 tgccagctag cttttcgcta agcatttttt gcaaaccata atctaaatgg agtaccaaat
     1201 cattgccgcg agttggttct gtgcgctcca atagccttaa tacttggcca tgagcattag
     1261 cttcaatttg ttgataacct gctttgcctt gcaaaataga ctcataatat ttttctaagc
     1321 ctgtcttgcc aatcaagtct gtaccagcat agcgttcagc gttaagggtt gcagcttctt
     1381 tgtcagaaat ccttcccaca taccctaaaa catgggcaaa taactcccca taaggataat
     1441 aacgtgctag tttaacctcg atatttacgc ctttaaataa aaaagcgact tctgaaaagc
     1501 gagcaatatc agattcgcgt aaatttaatt taatggcaac cgattcatag cgtggtgcat
     1561 ttttgactct atttttaaaa cgctgaatat cttcaggtgt tagctgaaaa ataggcgtta
     1621 aacgttcaat agtttgatct aaattatcaa tttcttgacg attgaccatt gcggtaaaaa
     1681 tagggcgatt atcggctaat aaaataccat tacggtcata aatataacca cgtggaggcg
     1741 caatcgcttg tagtttaata cggttactgt cggcaagcgt tttataattt tggtgttcta
     1801 agatttgtaa gtagccataa cgtattaaaa gggtactaaa tgccagtgca ataaaaaagg
     1861 cagtcaccca aatacgatta cgaaaaatac ggctttcgag agcactattt tttagtgggt
     1921 ctggattggt tgggctagga cgtgatttga ggcgcaaagt acaaaatcct aatc
//
LOCUS       DAOQMB010000434         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_39886_length_1970_cov_4.049608, whole genome shotgun sequence.
ACCESSION   DAOQMB010000434 DAOQMB010000000
VERSION     DAOQMB010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39886_length_1970_cov_4.049608"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1178)
                     /locus_tag="PLY05_10780"
     CDS             complement(<1..1178)
                     /locus_tag="PLY05_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HNC03823.1"
                     /translation="MTSLFHLFPRRHPSLPQNDNTNGQKHRAEQLLNSQDKYQWTETL
                     PNVVGVPMATEVHFSDEPTLEWLLLVSKTGLEIAENFLAVKLDQLDDDESTETQAKLA
                     NIHKLLQEIEALQQHNHEPSAIETIIHTTRQLLQQHNISFDGLLKQFKELSQDTDLSG
                     LSPDYLESYQQLFKTIPLPAIAKTFIDDESFARYRVAGPNPMLIQKLTEIPANFAFNN
                     QQYQQVMGQQDNLNLALAESRIYFIDYKELSLLADHTGQSSQGQTKYVYAPMALFAIP
                     QGGKSLVPVAIQCGQDHNQYPLFLQADSKQPLAWWGWQIAKTIVQVAEGNYHELFVHL
                     ARTHLVLEAFTVATHRHLAECHPVNILLLPHCEGTLFINNSAANSLIAPNAPIDHIFG
                     A"
     gene            1447..>1970
                     /locus_tag="PLY05_10785"
     CDS             1447..>1970
                     /locus_tag="PLY05_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03824.1"
                     /translation="MKKIITLASLLVSASTCFADSAWPLSQAMELQADTPVNRLQILG
                     AHNAWNDSSATWANQVWALDKLMDNGVRNIDLDIHNDGGVVKLCHQDCGAIYAAVDSY
                     PNELQKIANWLAAHPKEIIFIDLEDRVGNQSLVEAPLKSIFGSMLYLPSDKPSNRWET
                     PREMIAKGKRVIVKG"
BASE COUNT          579 a          331 c          415 g          645 t
ORIGIN      
        1 gcgccaaaaa tatgatcaat aggcgcatta ggcgcaatta aactattggc tgcggagtta
       61 ttaataaata aagtcccttc acaatgaggt aatagcaaaa tattaacagg atgacattcg
      121 gctaaatggc ggtgtgtagc aacagtaaat gcttctaaaa ccaaatgtgt acgcgctaaa
      181 tggacaaaaa gttcgtggta gttaccttca gcaacttgca caatagtttt ggcaatttgc
      241 caaccccacc atgctaaagg ttgtttgctg tctgcctgta aaaataatgg gtattggttg
      301 tgatcttggc cacattgaat ggcaacaggc accagtgact tacctccttg tggaatggca
      361 aagagagcca ttggcgcata aacgtatttt gtctgacctt gactactttg gcctgtatgg
      421 tcggctagta aagataattc tttataatca ataaaataga ttctactttc tgctagagct
      481 agattaaggt tgtcttgttg ccccatgact tgctgatatt gctgattatt aaaagcaaaa
      541 ttagcaggga tttctgttag tttttgaatc agcattgggt tagggccagc aacacgataa
      601 cgtgcaaaac tttcatcatc aataaaggtt ttagcaatag caggcaatgg aatggtttta
      661 aagagttgtt gataactctc aagataatca ggagataaac cacttaaatc tgtatcttgg
      721 ctgagttctt taaattgttt aaggagtccg tcaaaactga tattgtgttg ttgtagcaat
      781 tgtcgagtgg tatgaataat ggtttcaatg gcacttggtt catgattatg ttgttgtaag
      841 gcttcaatct cttgcaaaag tttatgaata ttagcgagtt ttgcttgggt ctctgtagac
      901 tcatcatcat ctaattggtc aagtttaact gctaagaagt tttcggcaat ttctaggcct
      961 gttttgctga ctaataataa ccattctaaa gttggctcat cagaaaaatg tacttctgtg
     1021 gccattggca cgcctacaac atttggcagg gtttctgtcc attgatattt gtcttggcta
     1081 ttaagcagtt gttcagcacg atgtttttgg ccgttagtat tatcattttg tggtagcgat
     1141 ggatgacggc gtggaaataa atgaaataag gaagtcatag tcatatccct tgataaatga
     1201 gtggtttaaa aaatatcagg gatcgttttg cataaataaa gcgttagact tttttattgt
     1261 atcttccctg acatgtttaa tttggtcatt ttttttataa aagcaagcag ttttttatta
     1321 actttgtatg ccaataaaag cttacttatg acgccaagta tcaatcttta atctatgcta
     1381 attagtacaa tttttgccca taatttatgc ttgctgcata gcatccccgc atattagagg
     1441 gttatcatga aaaaaattat tacgcttgct agtttattag tctctgcctc aacctgtttt
     1501 gccgattctg cttggccatt aagccaagcg atggaattac aagccgatac gcctgtcaat
     1561 cgtttgcaaa ttttaggcgc gcataatgcg tggaacgatt cttcagcaac ttgggcaaac
     1621 caagtgtggg ctttagataa gttaatggac aatggtgtgc gtaatattga cttagatatt
     1681 cacaatgatg gtggggtggt taaattatgt catcaagatt gtggcgcaat ttatgccgct
     1741 gttgatagct atcctaatga attacaaaaa attgctaatt ggttagcagc acatcctaaa
     1801 gaaattattt ttattgattt agaagaccgt gtgggtaatc aatccttagt ggaagcaccg
     1861 ctcaagtcga tttttggttc aatgttatat ttgcccagtg ataagccaag taatcgctgg
     1921 gaaacaccac gcgaaatgat cgccaaaggc aaacgtgtca ttgttaaagg
//
LOCUS       DAOQMB010000435         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_40005_length_1967_cov_4.827929, whole genome shotgun sequence.
ACCESSION   DAOQMB010000435 DAOQMB010000000
VERSION     DAOQMB010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40005_length_1967_cov_4.827929"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..>1967)
                     /locus_tag="PLY05_10790"
     CDS             complement(219..>1967)
                     /locus_tag="PLY05_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03825.1"
                     /translation="RGYLFIMSVLMLVVAVVEGVSWGYFGTQMSDYPLVSGTVMGVLV
                     FLFIWLFDRTLMTADYLQEEHAATLRGKAYSLNNADSNTKLGILFKYFNKHKVFLIRI
                     IIAISSLIIVAPYVSELSFYQEIKNKQHSYFLQEVTLVKQQHIQQKQQAIDSLAQQIA
                     TLNRKYQDETSGKLSGVRGRGAAAKAIEQELHSLQAQHQQLTTALQHYISRVEQAVVQ
                     HNLSELAALDIKIRQDSAILRKQAVQDIKTQHPQEYWQIEMTIRVLLAMFAIGLFSMK
                     WLQPRSLKLYFSSQLQQQWSLYCLGKYDAVLPSQQRRDWLLNSQDAVPEEFEQIIIAC
                     MNNFTAQAEYEANQQQLAKQRETAQQLHQQRMAHNHAHRQHLEQERAFFEQNTASRIA
                     EIDNLEHNYVAQHGQKIEQLKTQEQQLIDDLHVLEKEHKTQQDRVKAREFRIGLGVKE
                     YHDIQELLQQTRERPDSDTIEVLRTIADLENGLRSQQERLDRQRAELLGFEANQQCFE
                     ENHRLLHNRLLDIQSQLHDLQQPLQALNHARAQIEQKRIECLLAQGLHDSPFKAFNEQ
                     ELPHIMTTLSKAITLN"
BASE COUNT          557 a          375 c          398 g          637 t
ORIGIN      
        1 ccgcgccatg aaacaatcgc gtggaacata ataaaaacta ataaaaagca ggtttaaata
       61 gtttttggct attttattaa agaacctaaa gctgacgcat tttaccgtat cagctttata
      121 ataggggcgt tcccaagcct cttaatatac aagatgttaa gcgttttaac catcaaattg
      181 tgaccaaaaa aaaatgatac tacatcgcat aaaaccactt agtttagggt gatggcctta
      241 gacagtgttg tcattatgtg gggcagttct tgttcgttaa atgccttaaa gggactgtcg
      301 tgtaagccct gtgccaataa acactcaatc cgcttttgtt caatttgtgc ccgagcatgg
      361 ttaagtgcct gtagtggctg ttgcaagtcg tgtaattggc tttgaatatc taacaagcgg
      421 ttatgcaaca aacgatgatt ctcttcaaaa cactgttgat tagcttcaaa acccaacagt
      481 tcggcacgtt gtctgtccaa acgctcttgt tggctacgca ggccattttc gaggtcggca
      541 atggtgcgta acacctctat tgtatcactg tcaggacgtt ctcgggtttg ttgtaataac
      601 tcttgaatat catgatattc tttgacacct aaaccaattc taaattcacg tgctttaaca
      661 cggtcttgtt gtgttttatg ttctttttct aatacatgta aatcgtcaat taattgctgt
      721 tcttgtgttt tgagttgctc aattttttgg ccatgttgcg ctacatagtt gtgttctaag
      781 ttatcaatct cggcaatacg gctagccgta ttttgttcaa aaaaggcacg ttcttgctca
      841 aggtgttgac gatgggcatg gttgtgtgcc atgcgttgtt gatgtaattg ttgggctgtt
      901 tcgcgctgct tggctaattg ttgttgattt gcttcatact cagcttgagc ggtgaaatta
      961 ttcatacagg caattatgat ttgctcaaac tcttcaggta ctgcatcttg actgtttaat
     1021 aaccaatcac gacgttgctg actgggtaac acagcatcgt atttgcccaa acaatataag
     1081 ctccattgtt gttgtaattg actggaaaaa tagagtttta gcgaacgagg ctgcaaccat
     1141 ttcatgctaa ataagccaat cgcaaacatg gctaataaca cacgaatggt catttcgatt
     1201 tgccagtatt cttgtgggtg ttgggtttta atatcttgaa ccgcttgttt gcgcaaaata
     1261 gcactatctt gacgaatttt aatatctagg gcggcaagtt cggaaagatt atgttgaaca
     1321 acggcttgct ctacacgaga aatatagtgt tgtaatgcgg tggtgagttg ctgatgttga
     1381 gcttgtaaac tatgtaattc ttgttcgata gcttttgctg cagcaccacg accacgcacc
     1441 cccgacaatt tacccgaggt ttcgtcttgg tatttgcggt taagtgtggc aatttgttgc
     1501 gctaaactat caattgcttg ttgtttttgc tgaatatgtt gttgtttaac aagcgttact
     1561 tcttgcaaaa aatagctgtg ttgcttattt ttaatttctt gataaaacga taactctgat
     1621 acgtaaggtg caacaataat taaagaacta atagcgatga taatacgaat caaaaaaacc
     1681 ttatgtttat taaagtattt aaataatatg cctagtttag tgttgctatc agcgttgtta
     1741 agcgaatagg ctttgcctcg taaagttgcc gcgtgttctt cttgcaaata atcggcagtc
     1801 attaaagtgc ggtcgaacaa ccaaataaac aaaaatacca aaactcccat gactgtgccg
     1861 ctgaccaaag gataatcact catttgcgtg ccaaagtatc cccaagaaac accttcaact
     1921 acagcgacga ctagcattaa aaccgacatg ataaataaat aaccacg
//
LOCUS       DAOQMB010000436         1962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_40216_length_1962_cov_8.869953, whole genome shotgun sequence.
ACCESSION   DAOQMB010000436 DAOQMB010000000
VERSION     DAOQMB010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40216_length_1962_cov_8.869953"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1962)
                     /locus_tag="PLY05_10795"
     CDS             complement(<1..>1962)
                     /locus_tag="PLY05_10795"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HNC03826.1"
                     /translation="TMTGNTGNNQLDGTAGNDTLTGGTGADTLIGGLGDDVLNGSDGN
                     DVFIGGAGNDIISGGRGSDTVYFNLGDGNDIIDNQTDDINTTTDVLVFGQGIALNDLT
                     FMRYNNSADSTVLINGTTDSILVKNYFSQPQYYNNIQLHFADGTVVTYQQLLHMGYVN
                     QGDDSNNALNGFWGNDTLIGGLGDDTLIGGLGTDSYQLGLGDDTVIYHYNQDGGDYVA
                     DDLTNEQINAGISDGIDTYKIFNVPNSPVIQFGIEANTERYPNLVLTIGDNNVNLGTQ
                     FADDWRDSVSVGWQGIYIENFFTRNPAGSNNGTPVLTAASAQDVFVFYSDDGVTEINR
                     LTGQEVYDFYFAPQNHAPAQTEALAILANGIEDTTYTLFASDLLQGYTDADGDTLSVI
                     NLQATHGSLVDNQNGTYSFTPNANFNGTINLSYQVSDGHGGIAEASNSFVLDAVNDIP
                     FISNMSGEVGEDGFITGGGGYYDVEGDVLSYRIIGDVPEGFVLDGNNGAFGFSYTPTV
                     ADQTLKVGESKQLSVQYVANDGQADSLPATIQITINGVNDAPTGSATAILSNTTANTT
                     YTLLASALLQGFSDVDGDTLSINNLMADHATVVNNSNGTFTLMLEANYSGVVGLSYQV
                     TDGHNSSVDATQSFNVVGGNNTTGTTGAD"
BASE COUNT          598 a          444 c          317 g          603 t
ORIGIN      
        1 tatctgcgcc tgttgtacca gtagtattgt taccaccgac aacattaaag gattgtgttg
       61 catcaacact gctgttatgc ccatctgtca cttgatagct taaaccaact acaccactat
      121 aatttgcctc taacattaaa gtaaaagtac cattgctgtt attaactacc gtggcatggt
      181 ctgccattaa gttatttatt gacagggtat caccatcaac atcactaaaa ccttgtaaca
      241 aggcacttgc caaaagtgtg taagttgtgt ttgccgtcgt gtttgacaat atagcggttg
      301 cactacctgt tggagcatca ttcacgccat taatagttat ttgaattgtt gcaggtaaac
      361 tgtctgcttg accatcatta gccacatatt gaacactaag ctgcttgctt tcacctactt
      421 ttaaagtttg atctgctact gttggtgtgt aagaaaaccc aaatgcaccg ttgttgccgt
      481 ctaacacaaa cccctcagga acatctccaa taatacgata acttagcaca tctccctcta
      541 catcataata tcctcctcca cctgtgataa atccatcctc acccacttca ccactcatat
      601 tagatataaa cggtatatca tttactgcat ctaaaacaaa gctattgctt gcctcagcta
      661 tgccaccgtg tccatcactc acctgataac ttaaattgat tgtgccatta aagttagcat
      721 tgggtgtaaa agagtaagtg ccgttttgat tatcgactaa gctaccatga gttgcttgta
      781 aattgatcac acttaacgta tcaccatcag catcggtgta gccttgtaat aaatcactgg
      841 caaataatgt atatgtagta tcttctatgc catttgctaa aatggcaaga gcttcggttt
      901 gagcaggtgc atgattttgt ggagcaaaat aaaagtcgta aacttcttga ccagtgagac
      961 gattaatttc agtcacgcca tcatcagaat aaaaaacaaa cacatcttga gcggatgccg
     1021 ccgttaatac aggagttcca ttatttgaac ctgcgggatt acgcgtaaag aaattttcga
     1081 tataaatacc ttgccatcca actgatactg aatctcgcca atcatccgca aactgagtgc
     1141 ctaaattaac attattatct ccaattgtta gtaccaaatt tggataacgc tcggtattag
     1201 cctcaatacc aaattgaatg actggagaat taggaacatt gaatatttta taagtatcaa
     1261 taccatcgct gatgccagca tttatttgct cgtttgttaa atcgtcagca acataatctc
     1321 caccatcttg attgtagtga tatataactg tgtcatcacc taagcctaat tgataactat
     1381 ctgtccctaa acctcctatt aacgtgtcat ctcctaaacc gccaatcagc gtatcatttc
     1441 cccaaaaacc attgagagca ttattgctat catcaccttg attaacatag cccatatgca
     1501 aaagctgctg ataagtgacg actgtaccat cggcaaaatg cagttgaata ttgttgtaat
     1561 actgaggttg gctaaaatag tttttgacta aaatgctgtc cgttgtacca ttaattagca
     1621 cggtagaatc agcactattg ttataacgca taaaggttaa atcatttaaa gcaatacctt
     1681 gcccaaacac taaaacatct gtggttgtat taatatcatc ggtttgattg tctatgatgt
     1741 cgttaccatc acctaaatta aagtaaacgg tatcgctgcc tctgccacct gaaataatgt
     1801 cgttacctgc acctccaata aatacatcat taccatcact gccatttaat acatcatcac
     1861 ccaaaccacc aatcagtgta tctgcacctg taccacccgt taaggtgtca ttacctgctg
     1921 tgccatcgag ttgattatta cctgtattgc ctgtcatagt at
//
LOCUS       DAOQMB010000437         1954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_40526_length_1954_cov_5.097420, whole genome shotgun sequence.
ACCESSION   DAOQMB010000437 DAOQMB010000000
VERSION     DAOQMB010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40526_length_1954_cov_5.097420"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..686)
                     /locus_tag="PLY05_10800"
     CDS             complement(24..686)
                     /locus_tag="PLY05_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03827.1"
                     /translation="MNVSANNHLASFAQIVKNSTQRFAALPAHVQWDIYSTLKPNHYI
                     CQHESQLMAYNALFANQHFAKLIDLFDFVFGRFIEIEQITALQNTTQPISVIDYGCGQ
                     GLASMVLQEFNRERQLGYQFEEVILIEPSALALKEAVKNNSLYYTNIKQYCRKFDQIT
                     ADDLHTKHQVTIHLLSNVLDLPNIDVTALASCIKPSLSGVNIFVCCSPNFSNAVNGIK
                     RF"
     gene            complement(836..1210)
                     /locus_tag="PLY05_10805"
     CDS             complement(836..1210)
                     /locus_tag="PLY05_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNC03828.1"
                     /translation="MLIKLVRIGRDAEFRLANGKPLLNVSVVYDVGFGQHKKPQWLNL
                     TLWGQQAEKVQPYFTKGKQIVIRADDIHIEEYNGKSSLKGTLIGFDFVQNAPAQEAPK
                     PNNPPPTHQPPPPFDDLYDIPF"
     repeat_region   1322..1921
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1322..1357
                     /rpt_unit_seq="gtcttaatcccttagtaatcagggaagagttttaac"
BASE COUNT          619 a          338 c          399 g          598 t
ORIGIN      
        1 cagttaatgg cattaaacga ttttcaaaat cgtttaatgc cattaactgc attgctaaag
       61 ttggggctac aacatacaaa gatatttacg ccagataaac ttggtttaat acaactagca
      121 agagctgtga catcaatatt gggtaaatct aaaacattag ataataaatg aatagtcacc
      181 tgatgtttgg tgtgtaaatc atcagcagta atttggtcaa atttgcgaca gtactgctta
      241 atgttagtgt agtacagtga gttgttttta acggcttctt ttaaagccaa agcggaaggc
      301 tcaatcaaaa ttacttcttc aaactgatag cctaattgac gttcacgatt aaattcctgt
      361 aacaccatac tcgccaaacc ttggccacaa ccatagtcaa tgacactaat cggttgagtg
      421 gtattttgta atgcggtaat ttgttctatt tcaataaagc gaccaaaaac aaaatcaaat
      481 aaatcaatta atttggcaaa atgctgattg gcgaataggg cattatacgc catcaactgg
      541 ctttcatgtt ggcaaatata gtgattaggt ttgagcgtgc tataaatatc ccattgcaca
      601 tgagcaggca aagcggcaaa acgttgagtg ctgtttttga cgatttgagc aaaactggcc
      661 aaatgattat ttgcagaaac gttcattgca acacccttat aaatagtcgt taagaagtta
      721 acgaataatt tatcagggcg tgtgtgcaaa tatggttttt gcaagcttgc aaggattgtt
      781 agaaaaaagc caaaaacaat taacataaac ccgctgttgg cgggtttggt gtagtttaaa
      841 acggaatgtc gtaaaggtca tcaaaaggag gtgggggttg atgggttggg ggtggattat
      901 tgggttttgg agcttcttga gcgggggcat tttgcacaaa gtcaaaacct attaatgtgc
      961 cttttagact acttttgcca ttatattctt cgatatgaat atcatcggca cgaataacaa
     1021 tttgcttgcc tttagtaaag tagggttgca ctttttcggc ttgttgcccc catagggtga
     1081 ggttgagcca ttgcggtttt ttgtgttgac caaagccaac atcatatacc accgagacat
     1141 ttaataaagg cttgccattt gccaaacgaa attcggcatc acggccaata cgtactaatt
     1201 tgattaacat attaactcct aatagcgttg cttttcctaa ttttaatttg agtggttaaa
     1261 gtcccataaa gtctgggaag aggttcgaac aaaaagtttg ggtcatcact gagtatgatc
     1321 ggtcttaatc ccttagtaat cagggaagag ttttaactgg gagcaatata cgctaccgat
     1381 aatctataca ggtcttaatc ccttagtaat cagggaagag ttttaacgct atttatagca
     1441 cagataacct atacagtgaa aagtcttaat cccttagtaa tcagggaaga gttttaacct
     1501 tgaccaaacc ataattgact catcaattag agagtcttaa tcccttagta atcagggaag
     1561 agttttaaca gcacagaaaa cgcacctagt taatggtgtg cttgggtctt aatcccttag
     1621 taatcaggga agagttttaa ctttttacaa gctatcagga tgcaaaaaac gcgggtgtct
     1681 taatccctta gtaatcagcg aagagtttta acactacttt taaggcggat tatcttgtac
     1741 ctgccttgtc ttaatccctt agtaatcagg gaagagtttt aacaacagca aattctagct
     1801 ttagtgcaat tggtgaagtc ttaatccctt agtaatcagg gaagagtttt aacgcaatat
     1861 gtgctagaga actttaccac tataagtctt aatcccttag taatcaggga agagttttaa
     1921 caccagtttg gggcgaaaac ttaactaagt catt
//
LOCUS       DAOQMB010000438         1950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_40678_length_1950_cov_4.928232, whole genome shotgun sequence.
ACCESSION   DAOQMB010000438 DAOQMB010000000
VERSION     DAOQMB010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40678_length_1950_cov_4.928232"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1312)
                     /locus_tag="PLY05_10810"
     CDS             complement(<1..1312)
                     /locus_tag="PLY05_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03829.1"
                     /translation="MFLPDQLEKAALGRANYQTEADYRIPKGLKLKDEFSRAFQIASA
                     QWKHFASQQERQDLDTYQLTQRFVVELLQDALGYSLVEINTITLAERIYPVTHMATHH
                     VPIIIAPYTLGLDDADERFAIVGSGIRKKSPFQLAQEFLNASQQHTWAIVSNGKQIRL
                     LRDSATLTRPSYVEFDIQDMLGGLRFAEFEACWRLLHVSRTYGGEAEANIWEMWRNDG
                     QSEGTRVREGLREGVTQALLTLGEGFLQHPANEPLRQALMDGRLSKDEYFRQLLRLVY
                     RLIFLFTVEERGLLHPQDDSAQAVAARSAYAQGYALARWRERCLQRRARNRFDDIWTG
                     IRIVFKGLAQGEPRLALPPLGGLFAQDQCPYLDTAVLSNAHILEAISQLRWSNLSGNL
                     APVDYRNMGSEELGSVYESLLELVPDVDISARHFGFVGLTTEGSS"
     gene            complement(1394..>1950)
                     /locus_tag="PLY05_10815"
     CDS             complement(1394..>1950)
                     /locus_tag="PLY05_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03830.1"
                     /translation="GMEKEQRIDFSQLHRSHPLVSLLAEHLLEEALLGDNPVAARAAV
                     TVTTEVDAVTTLYVLRLRHQLSYQKRGQSHHLMAEETVTIAVRGRVQPEWLTDEVASQ
                     LLLCQPSGNLNTAFMQSEITKALEFLQQNSRYLESLAHQRAEILLADHRRVREAARDI
                     GQYAVVPCLPIDVMGVYVLLPDEL"
BASE COUNT          560 a          472 c          399 g          519 t
ORIGIN      
        1 ggctactgcc ttcggtggtt aagcctacaa aaccaaaatg acgcgctgaa atatcaacat
       61 ctggcacaag ctccaacaag ctttcgtaca cagaacctaa ctcttctgaa cccatattgc
      121 gataatcgac aggcgcaaga ttacccgata aattagacca ccgtaattga ctaatggctt
      181 ctaaaatatg ggcattactt aatacagccg tatcaagata tgggcattga tcttgagcaa
      241 ataatccacc taaaggtggc aacgctaaac ggggttcacc ttgtgccaac cccttaaaca
      301 caatacgaat gcctgtccat atatcatcaa aacgattacg cgctcgacgc tgcaaacaac
      361 gttcacgcca ccgtgctaaa gcataaccct gagcgtaagc actacgcgct gccacggctt
      421 gggcagagtc atcttgaggg tgtaacaagc cacgctcttc cacggtaaac aaaaaaatca
      481 gccgataaac caaacgtaat aactgccgaa aatactcgtc tttactaagc cttccatcca
      541 ttaatgcttg gcgtagtggc tcattggcag gatgttgtaa aaagccttca cccaaggtta
      601 ataaggcttg tgttacacct tcgcgtaagc cttcacgcac acgcgtgcct tcgctttggc
      661 catcattgcg ccacatttcc caaatattgg cttctgcctc accaccataa gttcggctca
      721 catgcagcaa tcgccaacag gcttcaaact ccgcaaagcg taaaccacct agcatatctt
      781 gaatatcaaa ctcgacataa ctcggacgag ttaacgtagc agagtcacgt aataatcgta
      841 tttgtttacc gttactgacg attgcccaag tgtgttgttg gctggcattt aaaaactctt
      901 gggctaattg aaacgggctt tttttgcgaa tgccgctacc tacaatggca aaacgctcat
      961 cggcatcatc taaacctaat gtgtatggcg caataataat aggcacatga tgcgtggcca
     1021 tgtgggtaac aggataaatg cgttcagcga gtgttatcgt attgatttcg acgaggctgt
     1081 aacccagtgc atcttgtaat aactcaacaa caaaacgttg cgtcagttga tatgtgtcaa
     1141 ggtcttgacg ctcttgttgg ctggcaaagt gcttccattg tgcgctggca atttgaaagg
     1201 cgcggctaaa ctcatcttta agtttgagtc ctttaggaat acggtagtct gcttctgttt
     1261 gataattggc acgacctaaa gcagcttttt cgagctggtc tggtaaaaac aaactgccct
     1321 ctaaacgtag cgttggcatt gacagcgtgg atactatttt ttttcgtata attcgcataa
     1381 ccgtgttaat cccttaaagc tcgtcgggta ataaaacata aacacccatt acatctatgg
     1441 gcaaacaagg aacaacggca tattggccaa tatcacgggc agcctctcgc acacgacgat
     1501 ggtctgcaag taaaatttcg gctcgttgat gtgccaatga ctctaagtaa cgactatttt
     1561 gctgtaaaaa ttctaaggct ttggtaattt cgctttgcat aaaagcggtg tttaaattac
     1621 cacttggttg acacaacaat aattggctcg ccacttcgtc agttaaccat tcaggttgaa
     1681 cacgaccacg cacagcgatt gtcactgttt cttcggccat caagtgatga ctttggccac
     1741 gcttttgata actcagttga tggcgcaaac gtagtacata caaggtagta acggcatcaa
     1801 cctctgttgt cacagtaacg gctgcacgtg ctgcaacggg gttatcacct aacaaagcct
     1861 cttctaataa atgctcggct aatagcgata ccaacggatg acttcgatgt aattgactaa
     1921 aatcaatacg ctgttctttt tccatgcctt
//
LOCUS       DAOQMB010000439         1949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_40714_length_1949_cov_5.438754, whole genome shotgun sequence.
ACCESSION   DAOQMB010000439 DAOQMB010000000
VERSION     DAOQMB010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40714_length_1949_cov_5.438754"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLY05_10820"
     CDS             complement(<1..325)
                     /locus_tag="PLY05_10820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNC03831.1"
                     /translation="MKIYIDPDTGREYSIDPTKCGAVTEPLITGIGNRVDLQDKYEGC
                     LLGLACGDAVGTTVEFKQRGSFAPVTDMVGGGFFDLKAGQWTDDTSMALCLAHSLLEQ
                     KGFDAK"
     gene            complement(456..>1949)
                     /locus_tag="PLY05_10825"
     CDS             complement(456..>1949)
                     /locus_tag="PLY05_10825"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNC03832.1"
                     /translation="LGLTTNNGTDVKVKLLKEESSLKKGVITLKSSQVLPQVITLIPN
                     DYINPQPIPTALQQVIEAISTHGNTTHCAIQHFLAKQPPKIAGHTDGAIVNATDSSKR
                     LEQIIQTVKKLNNSYLIIQGPPGAGKSYTAKHIIAELLKLGKKIGISSHSHKAINHLL
                     LTSVQYCQAQNIRATFVCTREDEHLTKLGVCVTKNSDLHTHLQNACVLGTTAWGFARE
                     DMINQLDYLFIDEAGQVAVANLIAMSRSAKNLILMGDQMQLGQPTQGSHPLQSGLSIL
                     DYFLGDTPTIANEMGVFLGESYRMHPLVNQFVSDYIYDGKLHTHADNVKQRIHVPSDY
                     QGELNKEAGIVFVPVEHQGNKQASDEEVDKIKLLVDELLTRQLTNKQGETRPVTLDDM
                     LFIAPYNHQVRKLQDKLGSQAKIGSVDKFQGQEAPIVFLSMCASDANDSPRGLDFIFN
                     KNRLNVAISRAKSLVVVVANKDLAIAKVNTIEQLKMVNLFCALVQEQ"
BASE COUNT          571 a          407 c          329 g          642 t
ORIGIN      
        1 ctttggcatc aaaacctttt tgctccaaca aactatgagc caaacataag gccatcgacg
       61 tgtcatctgt ccattgaccc gcttttaaat caaaaaagcc tccacccacc atatcagtca
      121 caggcgcaaa acttcctctt tgcttaaact caacagtagt acccacagca tcaccacaag
      181 ccaaacccag taaacaacct tcgtatttgt cttgtaggtc aactcgatta ccaatacctg
      241 taatcaatgg ttcagtaaca gcaccacact tggtagggtc aatactatat tcacgtccag
      301 tatcagggtc tatgtagatt ttcataatca actcaattta ttgtcttagg gacactaaaa
      361 gtagtttaat agacttttta cactatttca ataccatcaa accaagaacg acttgttatg
      421 tcgttgttgg tttgatgggg ataatcatct atctattatt gttcctgtac caatgcacaa
      481 aataagttca ccatttttaa ttgttcaatc gtattgacct tggctatagc taaatctttg
      541 ttggcaacca caacaaccaa acttttagca cgcgagatag cgacattaag gcgattttta
      601 ttaaaaataa agtctaaacc gcgtggtgag tcgttggcat ccgaagcaca catacttaaa
      661 aatacaatcg gtgcttcttg cccctgaaat ttatcaacac taccaatctt agcctgtgaa
      721 ccgagtttat cttgcagttt tctgacttga tgattataag gggcaataaa taacatatca
      781 tctaaagtaa caggtcgcgt ttctccttgt ttatttgtca gttgccttgt taataactca
      841 tcaactaata atttaatttt atcaacttct tcatcagagg cttgtttatt gccttgatgc
      901 tcaacaggca caaaaacaat acctgcttct ttatttaact cgccttgata atcgctaggt
      961 acatgtattc gttgcttgac attatcggcg tgggtatgca actttccatc gtaaatataa
     1021 tcactgacaa attgattgac taatggatgc atacggtaac tttcgcccaa aaaaacaccc
     1081 atctcattgg ctatggttgg cgtgtcacct aaaaaataat ctaatattga caatccactc
     1141 tgtaaggggt gtgaaccttg cgtcggttgc cccaactgca tttggtcacc cattaaaatc
     1201 aagtttttag cactccgact catggctatt aaattagcaa cggctacttg acctgcctca
     1261 tcaataaata aatagtccag ttgatttatc atatcctcac gggcaaaacc ccatgcggtt
     1321 gttcctagta cacaagcgtt ttgtaaatga gtatgtaaat cactgttttt agtcacgcaa
     1381 acacctaatt tagttaaatg ttcatcttct ctagtacaga caaatgttgc ccgtatgttt
     1441 tgtgcttggc agtattgcac gctagttaac aacaaatgat tgatggcttt atggctatga
     1501 ctactaatgc caattttttt acctaatttg agtaattcgg caataatatg ctttgctgta
     1561 taagacttgc ctgcacctgg tggcccctga atgattaaat aactattatt caatttttta
     1621 acggtttgaa taatttgttc gagtcgtttt gatgagtctg tggcattaac aatagcacca
     1681 tctgtatggc ctgcaatttt tggcggttgt ttggctaaaa agtgttgtat ggcgcaatgt
     1741 gtggtgttgc catgagtgct aatcgcttca ataacttgtt gtaatgcagt tggaataggc
     1801 tgtgggttaa tataatcatt aggaattaaa gttataactt gcggtaaaac ctgactactt
     1861 tttagtgtaa tcaccccctt ttttaacgat gactcctctt ttaacaattt aactttaaca
     1921 tctgtaccat tgtttgtggt taatcccaa
//
LOCUS       DAOQMB010000440         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41084_length_1939_cov_4.070064, whole genome shotgun sequence.
ACCESSION   DAOQMB010000440 DAOQMB010000000
VERSION     DAOQMB010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41084_length_1939_cov_4.070064"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1939
                     /locus_tag="PLY05_10830"
     CDS             <1..>1939
                     /locus_tag="PLY05_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03833.1"
                     /translation="VADVNSLGGYVRTFEVTPNLTALAARGVSLAQLQMAIEANHRSD
                     GAGRLVQHEESILVRSDSRIRSIDELKQLLVASVNGVPIFVADVAEVKIGSLTRYGAV
                     TQNGQGEAVEGIVLGLRGANARLLVSEVRHQLQEIEASLPKGVTLKPFYDRGDLVNRA
                     IHTVTKSLAEAIVLVLVLLMLFLGNLRAALVVAVILPLSALGTFLLMRYFGLSANLMS
                     LGGLAIAIGLLVDAAVVVVENIEAHQAEIIEKKQQAMLPPLHVIYRAVQEVAVPVSAG
                     MVIIMLVFLPLLTLQGLEGKLFAPVALTIVFALSASLLLSLTVIPVLASFVLKAQVHH
                     TPWLVARLEAGYQRLLDWSFVHTKWVYTGAALALVGALALFPFIGKSFMPTMDEGDII
                     MQLEKLPSIQLNSSTAIDLDVQQRILAKVPDIQRIVARTGSDELGLDPMGLNETDTFL
                     VLKPKEQWQAKNKEQIIDQVRAVIADVAGVNFSFTQPIEMRTSEMLSGVRGDLAVKVY
                     GSDLAELSQIAEQIANILQGIQGSEDVMTLENSGVQYLQVQIDRVAAGRVGLSSEDIQ
                     AMLRTLVEGQTISSVLEQGKPTPLVMRGAEALRLSTETFSQLQLPLANGQSIALSSVA
                     KLVADEGVVKIERENASRMSVI"
BASE COUNT          494 a          366 c          507 g          572 t
ORIGIN      
        1 agttgccgat gttaatagcc taggcggtta tgtcagaacc tttgaggtaa caccgaatct
       61 caccgcttta gcggcgcgtg gtgtgagctt ggcacaatta caaatggcga ttgaagctaa
      121 tcatcgtagt gatggcgcag gccgtttggt gcaacacgaa gagtcgattt tagtgcgcag
      181 tgatagccgt attcgcagta tagacgaact caaacaatta ttagtggcga gtgttaacgg
      241 tgtgccgatt tttgtcgctg atgttgctga ggttaagatt ggtagtttaa cgcgttatgg
      301 tgcagtcacc caaaatggtc aaggtgaagc tgtcgaaggc attgttttgg gcttacgtgg
      361 cgcaaatgcc cgtttattgg ttagtgaagt ccgtcatcaa ctgcaagaaa ttgaagccag
      421 tttgcccaaa ggagtgactt taaaaccgtt ttatgaccgt ggcgatttgg tcaatcgtgc
      481 gattcatacc gtcaccaaat ctttagctga agcgattgtc ttggtgttag tgttattgat
      541 gttgttttta ggcaatttac gtgcagcttt agtggtggcg gtgattttgc cattatcggc
      601 attaggcacg tttttgttga tgcgttattt tggtttatcg gccaatttaa tgtcgcttgg
      661 tggtttggcg attgccattg gtttattggt cgatgcggct gtggtggtgg tagaaaacat
      721 cgaagcgcat caagctgaga ttatcgaaaa gaaacaacaa gcgatgctgc cgcccttaca
      781 cgttatttat cgtgcagtgc aagaagtggc tgtgcctgtg agtgcaggca tggtgattat
      841 catgttggta tttttaccct tactcacgtt acaaggttta gaagggaagt tatttgcacc
      901 tgttgcatta accattgtct ttgccttgtc tgcttcgttg ttgttgtcct taaccgtgat
      961 tcctgtgttg gcttcttttg tgttaaaggc acaagttcat catacgccgt ggctcgttgc
     1021 acgtttagaa gcgggttatc aacgtttgct ggattggtcg tttgtgcata ctaaatgggt
     1081 ttatacaggt gcagcactcg ctttagtggg tgcattagcc ttgtttccgt ttattggcaa
     1141 atcgtttatg ccaaccatgg atgaaggcga cattattatg caattggaaa aactgccatc
     1201 cattcaactg aatagctcaa cagccatcga tttagatgta caacaacgta ttttggcgaa
     1261 agtgcctgat atacaaagga ttgtcgccag aacaggctca gatgagctgg gtttagaccc
     1321 catgggttta aatgaaaccg acaccttttt ggtgttaaaa cccaaagagc aatggcaagc
     1381 caaaaataaa gaacaaatca ttgaccaagt gcgtgcagtg attgccgatg tcgcaggggt
     1441 aaattttagt ttcactcaac ccattgaaat gcgtacctca gaaatgttgt caggggtgcg
     1501 tggtgatttg gcggttaaag tgtatggctc agacttggcc gagttaagcc aaattgctga
     1561 gcaaattgcc aacattttac aaggcattca gggcagcgaa gacgtaatga ccttagaaaa
     1621 ttcgggtgtg caatatttac aagtccaaat tgaccgtgtg gccgcaggtc gggtgggttt
     1681 aagcagtgaa gacattcagg cgatgttaag aaccttagtc gaagggcaaa ccattagcag
     1741 tgttttagag caaggtaaac caaccccctt agtgatgaga ggggcagaag ccctacgttt
     1801 atccaccgaa acgtttagcc aattgcaact acctttagcc aatggccaaa gtattgcgtt
     1861 gtcctcagtg gccaagttag tagctgatga aggcgtggtc aaaattgagc gtgaaaatgc
     1921 ctcacggatg tcagtgatt
//
LOCUS       DAOQMB010000441         1933 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41331_length_1933_cov_5.461661, whole genome shotgun sequence.
ACCESSION   DAOQMB010000441 DAOQMB010000000
VERSION     DAOQMB010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41331_length_1933_cov_5.461661"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1933
                     /locus_tag="PLY05_10835"
     CDS             <1..>1933
                     /locus_tag="PLY05_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03834.1"
                     /translation="GDLIDGIQLKNFRFTGKTFYLDAKQLTLDIVWIGLFRKELLVEE
                     LHGQGVQLVLHGQANPNRTKLKHLNIPFKIILNEGVLTDAQINKRGLITPIQRLALYR
                     AQWKGEDLQLAELDFVHPNFSTQLSGHLAFKNDYPLDAKGLLFVPFWQQHHLKPIKIK
                     ALGDLGNLAIDLVSKPVALSGTIDVLQPQLKYAANLRWSYWQFPWFSNQQFVSRYGQM
                     NIRGNKQQLELSINTDLQSNLLPRGEYRAFAETDWHKIQLLPLEARTSLGGKLAIRGE
                     VSWPKNQGIHWQLKTDWSNVDVAKQWPLVKPYLPILTGQLQTQGMTSAQKSKLDLNAQ
                     WISGEQWQIKQANNNWFWHWQQNQQLQASWKNLQRSVASLANINSQHGTLHYVGNPKA
                     YQLDFAANMASAKTPVGDWQIHGIGQPHQFDFKKLNYQGSAGQLNAQAQVAWQQGIQL
                     NGHVALQDFQSQFWLPTWPAQLSGETNFLLKTNKNQKDIQLTQSRLTGLLKNRDFNFN
                     SSFLNVDLQKLLSKETPSFYSQQTQITWGKNQLAVEGGLQNNQWQLTLDNQLNDLNEL
                     LPSLEGKVNGVVVLQGEQHKPAIISNLWAEKLAYEPTNLAIDELSISGTLPELGNVAG
                     FLQIAAHNLTAANRVIPDLT"
BASE COUNT          651 a          333 c          374 g          575 t
ORIGIN      
        1 ggtgatttaa ttgacggcat acagcttaaa aattttcgtt ttacaggcaa aactttttat
       61 ttagatgcta aacaattaac attagatatt gtatggatag gtttgtttcg taaggaatta
      121 ttagtagaag aattgcatgg tcaaggtgtg caattggtgt tgcatggtca ggcaaaccct
      181 aatcgaacca agttaaaaca tttaaatatt ccattcaaaa ttattttaaa cgaaggggta
      241 ttaaccgatg ctcaaattaa taagcgtgga ctaattaccc ctattcagcg tttagcctta
      301 taccgtgcgc aatggaaagg cgaagattta cagttagctg aattagattt tgtccatcct
      361 aatttttcta cacagttaag cggccatctt gcctttaaaa atgattatcc tttagatgcc
      421 aaaggactgc tatttgtacc attttggcag caacatcacc ttaaacccat aaaaattaaa
      481 gccttgggcg atttaggtaa tttagcgata gatttggtct ctaaacctgt tgcgctgagc
      541 ggtacgatag atgttttaca gccacagtta aagtatgccg ctaatctacg ttggagttat
      601 tggcaatttc cgtggtttag caatcagcag tttgtcagtc gttacggaca aatgaatatt
      661 cgtggtaata aacaacaatt agaactgagt ataaatactg atttacaaag taatttgtta
      721 ccaagaggtg agtatcgtgc ctttgccgaa accgattggc ataaaattca gttactacct
      781 ttagaagcca gaacaagttt aggcggtaaa ttagcgattc gtggtgaggt cagttggcca
      841 aaaaatcaag gtatccattg gcaactcaag acagattggt caaatgttga tgttgccaaa
      901 caatggccgc tagtaaaacc gtatttgcct atcttaacag gtcaattaca aactcaaggg
      961 atgacctctg cacaaaaatc caaacttgat ctcaatgcac aatggataag tggtgaacaa
     1021 tggcaaatta agcaagctaa taataattgg ttttggcatt ggcagcaaaa tcaacaacta
     1081 caagcatcat ggaaaaatct gcaacgcagt gttgcttcat tagctaatat caatagtcaa
     1141 catggcactc ttcattatgt gggaaatcct aaagcctacc aactagactt tgctgcgaat
     1201 atggcaagtg ctaaaacgcc tgtcggtgat tggcaaattc acggtattgg tcagccacat
     1261 caatttgatt ttaaaaagct gaattatcaa ggtagtgccg gacaattaaa tgctcaggca
     1321 caagttgctt ggcaacaagg catacagctc aatggtcatg ttgcattgca agattttcaa
     1381 agtcaatttt ggttaccaac ttggcctgca caactgtcag gtgaaacaaa ttttttacta
     1441 aaaacaaata aaaatcaaaa agatatacag ttaactcaaa gtcgattaac tggtttatta
     1501 aaaaatagag attttaactt taatagttct tttttaaatg ttgatttaca aaagctttta
     1561 tctaaagaaa cccccagttt ttatagtcaa caaactcaaa taacttgggg taaaaatcag
     1621 cttgctgtag aaggtggctt acagaataat caatggcaat taaccctaga taatcaatta
     1681 aatgatttaa atgaattgct accaagttta gaaggcaaag tgaatggtgt tgttgtgctg
     1741 caaggtgagc agcataaacc agcaattatt agtaacttat gggcagaaaa acttgcatac
     1801 gagccaacta atttagctat tgatgaatta agtatcagtg gcacattacc agagctaggt
     1861 aatgtggcag gttttttaca aattgctgca cataatttga ctgcggctaa tcgagtcatt
     1921 cccgatttga cca
//
LOCUS       DAOQMB010000442         1927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41524_length_1927_cov_16.684829, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000442 DAOQMB010000000
VERSION     DAOQMB010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41524_length_1927_cov_16.684829"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..206)
                     /locus_tag="PLY05_10840"
     tRNA            complement(132..206)
                     /locus_tag="PLY05_10840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(171..173),aa:Glu,seq:ttc)
     gene            complement(226..1734)
                     /gene="gltX"
                     /locus_tag="PLY05_10845"
     CDS             complement(226..1734)
                     /gene="gltX"
                     /locus_tag="PLY05_10845"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005018016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNC03835.1"
                     /translation="MTVVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGQFILRIED
                     TDQVRSTRESEEQILASLKWLGLTWDEGPDIGGKHAPYRQSERSAIYKQHADQLVTDG
                     HAFHCFCDSHRLEALRQQQQADKQMLGYDGHCLQLSAQEVAEKLAAGESSVIRMKVPR
                     EGVCEIDDLLRGKIDIEWSNVDMQVLLKADGLPTYHLANVVDDHLMEITHVIRGEEWI
                     NSAPKHLLLYKYFGWQAPVLCHMPLLRNPDKSKLSKRKNPTSITYYQRMGYLPQAVLN
                     YLGRMGWSMPDEREKFSLTDMIEHFDLKRVSLGGPIFDVAKLSWLNGQWLKELSVPDF
                     MQAFQNWAINSDYLSLVAKQIQPRIETLSDAVNWASFFFAGTVNLSADKFTHKKLSPE
                     EIKRILQYALWDFEQLRIWNHDQISLVLSQLSAALGYKLRDFMFPFFVAIAGSPSSTP
                     VMESMGILGADLTFARLRHAIDILGGLSKNEQSEWQKHWSSLKSVDSTSEDL"
BASE COUNT          604 a          390 c          384 g          549 t
ORIGIN      
        1 tttagagtta ccaccaacat taagtacttt tttacgagcc atattatttt ccttacgccc
       61 aatttttata gatggccgat gataataatt taaaaacaaa aaagccagca taaagctggc
      121 tttttcaaat ttggtgatcc cacggggaat cgaaccccgg ttaccgccgt gaaagggcga
      181 tgtcctgacc gctagacgat gggaccgtat ctgaggacgc gaatattata ggtcttcaga
      241 ggtgctgtca acacttttta gtgatgacca atgcttttgc cattctgatt gctcattttt
      301 tgacaaacca cctaaaatat cgatggcatg acgcaaacga gcgaaggtta agtcagctcc
      361 cagaatgccc attgactcca tcacaggtgt acttgatggg ctaccagcaa tagcaacaaa
      421 gaacggaaac ataaaatcac ggagtttata acccaatgca gcactcaatt ggcttaacac
      481 caaactgatt tggtcatgat tccaaatacg taactgttca aagtcccata gtgcatattg
      541 aagaatgcgt ttaatttctt cagggcttaa ttttttatga gtaaatttat cggcactaag
      601 attaactgtt cctgcaaaga agaagctagc ccaattaacg gcatccgata aagtttcaat
      661 gcgtggctga atttgtttgg ccactaaact caaatagtca ctgttaatgg cccaattttg
      721 gaaggcttgc ataaagtcag gtacagaaag ctcttttagc cattgtccat ttaaccagct
      781 cagtttggcg acatcaaaaa taggcccacc caaagaaacg cgttttaggt caaaatgttc
      841 aatcatgtca gtaaggctaa atttttcacg ctcatcaggc atggaccaac ccatacgacc
      901 taagtagttc aacacagctt gtggcaaata gcccatacgt tgatagtagg tgatactggt
      961 tgggttttta cgtttactga gtttgctttt gtctggatta cgcaataaag gcatgtgaca
     1021 caacacagga gcttgccaac caaaatattt ataaagcaat aaatgtttag gtgcagagtt
     1081 aatccattct tcaccccgaa ttacatgggt aatttccatt aagtgatcat caacaacatt
     1141 ggctaggtga taagtaggta aaccatctgc tttaagcaaa acttgcatat ctacatttga
     1201 ccattcaata tcaattttgc cacgtaataa atcatcgatt tcacacacgc cttcacgagg
     1261 cactttcatg cgaataacgg agctttcgcc agccgccaat ttttcagcca cttcttgtgc
     1321 agaaagctgc aagcaatgac catcataacc aagcatttgt ttatctgctt gttgttgttg
     1381 gcgtaaagct tctaaacgat ggctgtcaca aaaacaatga aaggcatgac catcagtgac
     1441 taattgatcg gcgtgttgct tataaatagc actacgttca ctttgacgat aaggggcatg
     1501 tttgccgcca atatcagggc cttcgtccca tgttaagcct aaccatttta aggaagctaa
     1561 aatttgctct tcagattcac gggttgaacg tacttggtct gtgtcttcaa tacgtaaaat
     1621 aaattggccg ccatgctgtt tggcaaagca taaattgaat aaagcaatat aagccgtgcc
     1681 aacatgagga aaaccagttg gtgagggggc aatacgggta cgaaccacag tcataactgt
     1741 acctagcgaa tgaaaaaatt aaagatacgc gattatatca ggagttgaca agaagcgcga
     1801 aagcctgact tttgtgctaa agtattgata ggttattagt taaacagttt agttgaatat
     1861 tgaatgaaga gaatattatg ccgttaactg ctccaagttt agataaggat attttttggc
     1921 aattaat
//
LOCUS       DAOQMB010000443         1926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41587_length_1926_cov_3.392838, whole genome shotgun sequence.
ACCESSION   DAOQMB010000443 DAOQMB010000000
VERSION     DAOQMB010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41587_length_1926_cov_3.392838"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="PLY05_10850"
     CDS             <1..1087
                     /locus_tag="PLY05_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC03836.1"
                     /translation="QSFTICAVVTVIWVIVGYSLAFTPSASPYLGTLDRLFLEGMSFD
                     KLTSTLTVSHIAPTIPESVFVVFQLTFAIITPALITGAFAERIKFGALIIFMALWSVL
                     VYAPVAHWVWEPSGWLAKMGALDFAGGTVVHINAGAAGLACAYMLGKRAGYGREPFFP
                     NNLGLTLIGASLLWVGWFGFNAGSALAADGRAGMAMLVTQVAAATGTLAWMLLEWLNR
                     GKASLLGACSGAVAGLVAITPASGFVGVESALLIGAIAGFACYWGATGLKRILGADDS
                     LDVFGIHGIGGVVGALLTGIFAQKHFSGLETTMTIQAIGAFATLAYSFIVSWIILFIL
                     DITIGLRVDAEDESMGLDLTQHGEQIP"
     gene            1253..1687
                     /locus_tag="PLY05_10855"
     CDS             1253..1687
                     /locus_tag="PLY05_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03837.1"
                     /translation="MTEALNPEVFSLASQLYVRMRRCSGRVVDAVYMAKNEQYAQEIL
                     QIAAQEKDQEILDIVKRFEAILAKKSVDLKATMIPELPIVTDMVKESEQATEKTDIFS
                     MTFRNLRRKVADLPTVEQLNGAALHNDHLEEEVAHHYTGALR"
     gene            complement(1709..>1926)
                     /locus_tag="PLY05_10860"
     CDS             complement(1709..>1926)
                     /locus_tag="PLY05_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03838.1"
                     /translation="LLKPQERKTAIPIQLIIPTKDQFVTAGLFDDLTQWADRVWRHEI
                     DAGHWVQRSHPDEISAYIREFVDFLDQ"
BASE COUNT          524 a          314 c          473 g          615 t
ORIGIN      
        1 gcaaagtttt acgatttgtg cagtagtaac cgttatttgg gttattgtcg gctatagctt
       61 ggcctttacc ccgagtgcca gtccatactt aggtacatta gatcgtttgt ttttagaagg
      121 catgagtttt gacaaattaa caagtacttt aacagttagt catatagctc caaccattcc
      181 agaatcagtt tttgtagttt ttcaattaac gtttgccatt atcactcctg cattaattac
      241 tggcgcattt gctgaacgaa ttaaatttgg cgcattaatt atatttatgg cgttatggtc
      301 ggtgctggta tatgcgcctg ttgctcattg ggtatgggag ccaagtggtt ggttggccaa
      361 gatgggagcc ttagattttg caggaggcac cgttgtacat atcaatgcag gagctgctgg
      421 attagcatgt gcttatatgt taggaaagcg tgctggctat ggacgagagc cattttttcc
      481 aaataattta ggattaacat tgatcggagc gtcattattg tgggtaggat ggtttggatt
      541 taatgcagga tctgctttgg cagctgatgg acgtgcaggg atggccatgt tggtcactca
      601 agttgctgcc gcaacaggaa cattagcatg gatgctttta gagtggttaa atcgtggtaa
      661 agcttcgtta ttaggggctt gttctggcgc agttgcaggt ttagtagcca ttactcctgc
      721 atcgggtttt gttggtgttg aaagtgcgtt attaatagga gcgattgcag ggtttgcatg
      781 ttattgggga gcaacaggat taaaacgaat tttaggagca gatgattctt tggatgtgtt
      841 tggaatacat ggtattggtg gtgtggtcgg cgcattatta acagggattt ttgcgcaaaa
      901 acacttctca ggtctagaaa caacaatgac tattcaagcg attggtgcat ttgcaacgtt
      961 agcgtatagc tttattgttt cgtggattat cttgtttatt ttggatatta ctattggttt
     1021 acgtgttgat gctgaagatg aatctatggg tttagattta acgcaacatg gtgaacaaat
     1081 tccttaacct caaagtcagt ttgttattgg tttaacaagc actgtttagg ggcttacgca
     1141 gtttactgaa gttagcgcgt aagtccgatg ttgtgtcaga gtttggctat atagccagtg
     1201 tagccaaact gactctttaa tgcagtttct tctatgtgtg gagaaagcaa tcatgactga
     1261 agctcttaat ccagaagtat ttagtttagc gtcacaattg tatgttcgaa tgagacgttg
     1321 ttcaggacgt gttgttgatg cagtttatat ggctaaaaat gagcaatatg cacaggagat
     1381 tttgcaaatt gcggcacaag aaaaggatca agaaatttta gatatcgtga aacgttttga
     1441 agcgatattg gctaaaaaat ccgtagattt aaaagctact atgattccag agttacctat
     1501 cgtgacagat atggtaaaag agtcagaaca agcaaccgaa aaaacggata tttttagtat
     1561 gacctttaga aacttacgac gtaaagtggc agatctacca acggttgagc agttaaatgg
     1621 ggcagcactt cacaatgatc atcttgagga agaagttgca caccattata ccggcgcatt
     1681 acgttaaagt cagtgcaggg cactgacttt actgatctaa aaagtcaaca aactctcgaa
     1741 tgtatgcact aatctcatcg ggatgacttc gttgcaccca atgtccagcg tcaatttcat
     1801 ggcgccaaac acggtctgcc cattgggtta agtcatcaaa taaccctgcg gtgacgaatt
     1861 gatctttggt ggggataatt aattggatgg gaatagctgt tttacgttct tgaggcttta
     1921 gtaaac
//
LOCUS       DAOQMB010000444         1925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41615_length_1925_cov_6.579144, whole genome shotgun sequence.
ACCESSION   DAOQMB010000444 DAOQMB010000000
VERSION     DAOQMB010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41615_length_1925_cov_6.579144"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLY05_10865"
     CDS             <1..556
                     /locus_tag="PLY05_10865"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNC03839.1"
                     /translation="ADVVSKKQLNAPNILRELIALESNPWQQEKDWLNMPIPDLVHYL
                     VSYYHERHRQQLPELIRLAAKVERVHGDKADCPHGLAALLNDTLEDLEQHMLKEEEVL
                     FPLLVHGRLTQAQMPIYVMEQEHITHGQRLEKLLSLTNQGVPPKGACNTWQALYLGVR
                     SFSQDIVEHIHLENNILFARCLQD"
     gene            561..1121
                     /locus_tag="PLY05_10870"
     CDS             561..1121
                     /locus_tag="PLY05_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HNC03840.1"
                     /translation="MLRKNPRGDWPQVAQSAFIDPTAILCGQVIVGENVFIGPYAVIR
                     ADEVNDKGEMEPIVIGAHSNIQDGVVIHSKAGGLVVIGEYTSIAHRSIIHGPCTIGSH
                     VFIGFNSVVYNATIHDDVVVRHNCVIDSREILAGLYVPSCHKVNHETDTQLLKPASDY
                     AHDFSESVAKANHSLVQGYRAIRNEF"
     gene            complement(1137..1643)
                     /locus_tag="PLY05_10875"
     CDS             complement(1137..1643)
                     /locus_tag="PLY05_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC03841.1"
                     /translation="MTINSTHFCQVIAQDIMTTPVLTSYDTWSVKMLLDFFAQHRITG
                     VPVINYQGELKGVVTMSDILRFENLPAIEKERLIGVSCYGEYSNYQLSHHDLEQLVLH
                     ADINCPVSQIMTPKIITVSQSTSILEVAKLFLEKRIHRVFVEQNQKLVGVISTSNLLT
                     AMIKSCDC"
BASE COUNT          574 a          343 c          408 g          600 t
ORIGIN      
        1 ggccgatgtg gtgtctaaaa aacagctcaa tgcgcccaat attttacgtg agttaatcgc
       61 attagaaagt aatccatggc agcaagaaaa agactggctt aatatgccta tccctgattt
      121 ggtgcattat ttggtcagtt attatcatga acgacatcgt caacaattgc ctgagttaat
      181 tcgtttagcg gctaaagttg agcgcgttca tggcgataag gcagactgtc cacatggttt
      241 ggcggcttta cttaacgata ctttagaaga cctagagcag catatgctta aagaggaaga
      301 agtattattt ccactgttag tacatggccg tttaacgcaa gcccaaatgc ccatttatgt
      361 gatggagcag gagcatataa cgcatggtca gcgtttagaa aaattactgt ctttgactaa
      421 tcaaggtgta ccacctaaag gcgcgtgtaa tacatggcaa gctttatatt taggcgtgcg
      481 tagcttttcg caagatattg tggagcatat tcacttagaa aataacattt tgtttgcacg
      541 ttgtttacaa gattaaagct atgttacgca agaatccacg cggagattgg ccacaggttg
      601 cccaaagtgc atttattgac ccaacggcta ttttatgtgg ccaagttatc gtgggtgaaa
      661 atgtttttat tggtccttat gctgtgattc gtgccgatga agttaatgac aaaggagaaa
      721 tggaacctat tgtcatcggc gcacattcta atattcaaga tggggtggtg attcactcta
      781 aagcaggggg attagtggtg attggtgaat atacatcaat tgcccatcgc tccattattc
      841 atggaccttg tactattggt agtcatgtat ttattggctt taattcggtg gtttataatg
      901 ccacaattca tgatgatgtg gtggtgcgtc acaactgcgt gattgatagc cgtgaaattt
      961 tggcaggttt gtatgttcca tcatgtcata aagtcaacca cgagacagac actcaattat
     1021 taaaacccgc ctccgattat gcccatgact tttctgaaag tgttgccaag gcaaatcata
     1081 gtcttgttca gggatataga gccattcgga atgagttcta aaggggctga tagcccctaa
     1141 caatcacaac ttttaatcat tgcagttaat aaattgctcg tactaattac accaacaagt
     1201 ttttgatttt gttctacaaa tactcgatga attcttttct ctaaaaataa ttttgcaact
     1261 tctaagattg aggtagattg actcacggta atgattttgg gtgtcatgat ttgactaaca
     1321 ggacaattta tgtctgcatg taatactaat tgctctaaat catggtgact tagttgatag
     1381 tttgaatatt caccataaca agaaacacct attagacgtt ccttctctat agcaggcaaa
     1441 ttttcaaagc gtaaaatatc gctcatggta acaacacctt ttaactcacc ttgatagttg
     1501 ataactggaa caccagtaat acgatgttgc gcaaaaaaat ccagcaacat ttttactgac
     1561 caagtgtcat atgatgttaa tacaggtgtt gtcataatgt cttgcgcaat aacttgacaa
     1621 aaatgtgtgg aattgattgt cataatagct tgtccaacgt taaagaataa tcgtgtaagt
     1681 cttaatgatt ttcttagtat atgtttcttt ttaataactg atattagcct gacggctagg
     1741 acagattagg ctaaagtata gaaacatttg ggattctgcg cgtctatttc gtgtgcatcc
     1801 ttcaacggtg gtgagattgc tggcgaaaaa caggttaaac acgtctcaca acccttaacg
     1861 tactttatat tggggtctga ggcgcagtgg aacggaaaac cgagttaaga ggtcaatata
     1921 gatgg
//
LOCUS       DAOQMB010000445         1920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41821_length_1920_cov_13.702949, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000445 DAOQMB010000000
VERSION     DAOQMB010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41821_length_1920_cov_13.702949"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLY05_10880"
     CDS             complement(<1..1028)
                     /locus_tag="PLY05_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC03842.1"
                     /translation="MKIAVIGSGIAGLSAAWLLANKHQVTVFEANHYAGGHSNAVDVE
                     LDGQQAAVDTGFLVFNEKTYPNLIQLFSLLNLTIANTDMSFSVRLQQENLEWAGSNLA
                     SLFAQKRNIVRPKFWQMINDILRFNRQALGLLALAKAEQLSLGQLLHQEKYSQAFKDW
                     YLLPMGAAIWSTPSADMLDFPAESFIQFCLNHGLLQISDRPQWLTIKGSSRQYVKQLC
                     AGIGDVRLNTPVIAISSHEHGVHIQTEHEHLSFDKVILACHTDQSLALLQSPTPEQHS
                     LLSAIRYAPNQAYLHTDSSVMPKNRQAWAAWNYAQNSAQDTHQAVAVTYWLNKLQPLP
                     FTTPLFVTL"
     gene            1252..1917
                     /locus_tag="PLY05_10885"
     CDS             1252..1917
                     /locus_tag="PLY05_10885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNC03843.1"
                     /translation="MLHPSLEPALAQIPQDRPVILFTRHSLRELAPNNGVPSYDLPLT
                     PEGVLLAEQWGATLGKKISAFYSSPVGRCVDTALAMARGAGLELVIEQTKVLVEPGCF
                     VHSIRHVGPLFLQMGPVAFANHHLQHMMEEGILTPKEGAAKLIQHFYQSQQQQTAGNL
                     IVHVSHDTIIAAFIYHLLQKTQINEEDWPWMMEGAWLWFDNSSLYYLWRGIQGQVSLA
                     DYL"
BASE COUNT          584 a          353 c          391 g          592 t
ORIGIN      
        1 agtgtgacaa acaatggtgt ggtaaacggt aaaggctgta acttgtttaa ccaataagtc
       61 acagcaaccg cttgatgtgt gtcttgtgcg ctattttgag cataattcca cgctgcccac
      121 gcttgacgat ttttcggcat cactgagcta tcagtatgta aataagcttg attaggtgcg
      181 taacgaatcg cagacaatag tgaatgttgt tcgggcgtag gggattggag taaagctaaa
      241 ctttggtcgg tatgacaagc caaaatcact ttatcaaaag atagatgttc gtgttcagtt
      301 tgaatatgca cgccatgctc atggctgctt atagcaataa caggcgtatt taaacgtaca
      361 tcaccaatgc cagcacagag ttgtttaacg tattgtcgag agctaccttt gatggttaac
      421 cattgtggcc gatcactgat ttgtaataaa ccatgattta aacaaaattg aataaatgat
      481 tcggcaggaa aatctaacat atccgccgaa ggtgttgacc aaatcgctgc acccataggc
      541 aataagtacc aatctttaaa cgcttggctg tatttttctt ggtgcaaaag ttggcctaaa
      601 cttaactgtt cagctttggc tagggctaat aaacctaatg cctgacgatt aaaacgtaaa
      661 atatcattaa tcatttgcca aaattttgga cgaacaatat tgcgtttttg ggcaaataaa
      721 ctggctaaat tgctgccagc ccactctaag ttttcttgtt gtagacgcac gctaaaactc
      781 atatcggtat tagcaatagt taaatttagt aaagaaaata actgaattaa atttggataa
      841 gttttttcgt taaatactaa aaaaccagta tccaccgcag cttgttgacc atccaactca
      901 acatcaacag cattactatg accaccagca taatgattgg cctcaaatac cgttacttga
      961 tgtttgttgg ctaataacca tgctgcgctt aagccagcaa ttcctgaacc aatcactgca
     1021 attttcattt tttgtcctat acaaaatttg ctgtttgagt attaatagcc tatttttggt
     1081 ttttatgcaa atttttgtat aatacaaaat gtaattttgc gtaaacaggc tattgactta
     1141 tatacgacaa aaaatagtaa atggatgcaa aaaagcctga aaataaaaaa gagcagataa
     1201 gtagatgcgt tatactgagc aacaatcaca tttgctgttt gatttacgat tatgttacac
     1261 ccaagtttag aacctgcttt agcgcaaatt ccccaagatc gacctgttat tttatttacc
     1321 cgtcattccc tacgagagtt agcacccaac aatggtgtgc ctagttatga tttgccatta
     1381 acgccagaag gtgtgttgtt ggctgagcaa tggggggcaa ctttaggcaa aaaaatcagt
     1441 gctttttatt ccagtccagt cgggcgttgt gtggatactg ccttagcgat ggctagaggt
     1501 gcaggtttag agttggtgat tgagcaaacc aaagtcttag ttgaaccggg ttgttttgtg
     1561 catagtattc gtcatgttgg tccgttattt ttgcaaatgg ggccagttgc ttttgctaat
     1621 catcatttac aacacatgat ggaagaaggc attttgacac ctaaagaagg tgctgccaaa
     1681 ctcattcaac atttttatca aagccaacag cagcaaacag caggaaattt aattgttcat
     1741 gtttcgcatg acaccattat tgctgccttt atttatcatt tattacagaa aacgcagatt
     1801 aatgaggaag attggccgtg gatgatggaa ggggcatggt tgtggtttga taattcgtcc
     1861 ctatattact tatggcgtgg catacagggt caagtcagct tggcagatta tttataactc
//
LOCUS       DAOQMB010000446         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41908_length_1918_cov_16.601718, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000446 DAOQMB010000000
VERSION     DAOQMB010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41908_length_1918_cov_16.601718"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..570
                     /locus_tag="PLY05_10890"
     CDS             205..570
                     /locus_tag="PLY05_10890"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNC03844.1"
                     /translation="MTKPLSEFAQFCLEMMALLAPVSAKKMFGGYGIYKNGVMFALIV
                     NDVLYLKTNLNTKSLFIEKQLEPFTYQKQGKTQTIAYYQCPEEAFDDETIMLSWAKTA
                     YLVAIEAASSENKHKSKIV"
     gene            659..1492
                     /locus_tag="PLY05_10895"
     CDS             659..1492
                     /locus_tag="PLY05_10895"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC03845.1"
                     /translation="MRHKTFEFMSRGKTFFAQRWWPDTYPKAVVIFIHTWAGHSSRHL
                     PLIERLTQLGYACYGFDFMGHGHTQGKRAYIPDFDYWLADLTTFIEVVHSELRSVPFF
                     LHGYGVGATAAANYVVSGHTDVDGIIFNSGALAVGKKIAKVHIVLAWIIGRVLPIIPI
                     APLPPNTMSTLADKQQAYDTDPLIYHGQMAAGTGKELLMASMYISQRLHKIAVPFLAL
                     QGRQDTLVTGSEQLYQQASSSDKTLKSYDALHDLLNDKPAEQVITDIIEWLESRVVVH
                     H"
     gene            complement(1489..>1918)
                     /locus_tag="PLY05_10900"
     CDS             complement(1489..>1918)
                     /locus_tag="PLY05_10900"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC03846.1"
                     /translation="PLVNYQSLLVLGKQHELNQKAYIEPQDLLNQTLIAYPVDLARLD
                     IFKHFLLPANIKPAQLRTTELTLMMIQLAASHRGVCALPDWVAKEYVQRGWIKTQKLG
                     EHGVWCTLYAAIRHEDINLPFMKDFIESAKLSLAMMQKSD"
BASE COUNT          609 a          332 c          379 g          598 t
ORIGIN      
        1 accttaaagc cttgttgatg taaacacagt gctgcggtta agccgccaat gcctgcgcca
       61 ataattgcaa tttgcatgaa gattcatctc aatgatataa aaatattaat tataaaacta
      121 acttaaataa accaataaaa acataattat tttaaggttt ttaaggttaa aatagaaaat
      181 atagatcatc tatgagtcaa actaatgact aaaccactgt cagaatttgc ccagttttgc
      241 ctagaaatga tggctttgtt agcgcctgtt tcggctaaaa agatgtttgg tggttatggt
      301 atttacaaaa atggcgtgat gtttgcctta attgttaatg atgtgctgta tttaaaaacc
      361 aatctcaata caaaaagctt gtttatcgaa aaacagcttg agccatttac ttatcaaaaa
      421 caaggtaaaa ctcaaactat tgcgtattat caatgtccag aagaagcttt tgatgatgaa
      481 actattatgc tgtcgtgggc aaagactgct tatttagtag ccatagaagc cgcatcgagt
      541 gaaaacaagc ataaaagcaa aattgtatga ccatcttggc aataaaaaaa tggcatttag
      601 ggtaaagtag taaacaaaac aaaaagttag aatataaaaa tttaaggagt tcaactaaat
      661 gcgccataaa acctttgagt ttatgagtcg tggaaaaact ttttttgccc agcgttggtg
      721 gccagacact tatcctaaag cagtggtcat ttttattcat acatgggcag gacattcctc
      781 tcgccatctt ccattgattg agcgtttaac tcagttaggt tatgcctgtt atggttttga
      841 ttttatgggg catggtcata ctcaaggcaa acgagcctac atcccagatt ttgattattg
      901 gttagccgat ttgacgactt ttattgaagt cgtgcattcg gaattacgta gcgtgccatt
      961 ctttttacat ggttatggtg taggtgcaac agcagcggca aactatgtgg tttctggtca
     1021 taccgatgtt gacggtatta tttttaatag tggtgctttg gcggtaggta aaaaaattgc
     1081 taaggtgcat attgttttgg catggataat tgggcgagtg ttaccgatta ttccgattgc
     1141 tcccttacca cctaatacca tgtctacctt agcggataaa caacaagctt atgatacaga
     1201 ccccttaatt tatcatggtc aaatggctgc agggacaggc aaagaattat taatggccag
     1261 tatgtatatt agtcaacgtt tgcataaaat agcagtgcca tttttggcct tgcaaggtcg
     1321 gcaagatact ttagtgacag gcagtgagca attgtatcag caagccagta gcagtgataa
     1381 aaccttaaaa agttatgatg ctttacatga tttgttaaat gataaacctg ccgaacaagt
     1441 catcactgac attattgaat ggttagaatc acgagttgtt gtgcatcatt aatcactttt
     1501 ttgcatcatc gctaaactaa gtttggcact ctcaataaaa tctttcataa atggcaaatt
     1561 aatatcttca tggcgaatgg cagcatataa agtacaccaa acgccatgtt cccccaattt
     1621 ttgcgttttg atccaaccac gttgcacata ctcttttgcc acccaatcgg gcaatgcaca
     1681 aacgcctcga tgactcgccg ctaattgaat catcattagg gttagttcgg tggtgcgtag
     1741 ttgtgctggt ttaatattgg cgggtaataa aaaatgttta aaaatatcca aacgggctaa
     1801 atcaacagga taagcaatta aggtttggtt aagtaaatct tgtggctcta tataggcttt
     1861 ttgattaagt tcgtgttgtt tgcccaatac taacaaagac tgataattca ctaagggt
//
LOCUS       DAOQMB010000447         1917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_41951_length_1917_cov_9.661654, whole genome shotgun sequence.
ACCESSION   DAOQMB010000447 DAOQMB010000000
VERSION     DAOQMB010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41951_length_1917_cov_9.661654"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(296..>1917)
                     /locus_tag="PLY05_10905"
     CDS             complement(296..>1917)
                     /locus_tag="PLY05_10905"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NERD domain-containing protein/DEAD/DEAH box
                     helicase"
                     /protein_id="HNC03847.1"
                     /translation="DISEVKAIGNIAEQKVLSALSSLPSPWQIFNTVEWRNLGNYGEE
                     VGEADVVVFNPQYGLIIFEVKAGSVYLKDGQWFYASGRPMKQSPFSQARRNRFALIEK
                     LEKRLGKTTVEHLAITHAVWFPDVKWPKQLVIAEAPSSHFIFDRHTLSNPLPQLEKMF
                     AEVKQASSPVWTKIQQNALKELLAPDCLLLVPLANELDESVTQLHQATEQQIRVLRLL
                     RTQKRLLIEGGAGSGKTLLATMMAREHAQQGKKILFTCYNKALALQLAEQLSAYKQQI
                     TVLHFHELVRFCALQAGLTYQVPADEQQRRHFFNDDCPELLMAAAEKIQDRYDSLIVD
                     EAADMSPTWWIALESLMNPDFSWYCFYDTYQTIFHDNQQWQAPFQGEVMALDVNLRNT
                     QPIGEFAVRLGKCPIPTFAVTTGQNPHLTICPSFDAMADALRQTLHKLMNEEAVTPER
                     IVILSPYRHTNPQSTWAAGLKDVDLHTEMNVIVKGKIRIGTIQSFKGLESDVVILVGL
                     TQHSVKQSELLYVAASRAKAALYVFSLYALS"
BASE COUNT          559 a          400 c          360 g          598 t
ORIGIN      
        1 tggattatta ctgaggccga taggtcggtg actacggtgt tgttgccaga ggagtattaa
       61 agcgcggcat aaataaagca gtctgcccat ctaaggacgg actgctttat tgtttatttc
      121 tatgaaagtt ctaaatcacc aagtcgttta ttttcagtgt aataatttca ttatcaacaa
      181 catcagccat aaactgtcat attgcctcta aatacagcat atctcatcta gttgtactaa
      241 gactaaaatc aattatgtaa ggggtatttt attttttgct ttaaatatca aaaaactacg
      301 acaaagcata caatgaaaac acatataaag cagcctttgc tcgactagct gccacatata
      361 aaagttcact ttgcttcaca ctgtgttgcg ttaaacccac caaaataact acatcagatt
      421 ctaaaccttt gaaactttgt atcgtcccta tccgaatttt ccccttcacg atgacattca
      481 tttcggtatg taaatcaacg tcttttaatc ctgctgccca tgtcgattgc ggatttgtat
      541 gccgatacgg tgaaagaatg acaatacgct caggagtaac tgcctcttca ttcatcaatt
      601 tgtgcagtgt ttggcgtaat gcgtccgcca tagcatcaaa acttgggcaa atcgtcaggt
      661 gtggattttg gcctgttgtc accgcgaatg tgggtatagg acattttcct aaacgcaccg
      721 caaactcacc aatgggttga gtgttgcgta aattaacgtc taatgccatt acctcaccct
      781 gaaatggagc ttgccattgt tgattatcgt gaaaaatcgt ttgataggta tcgtaaaagc
      841 agtaccaact aaaatcggga ttcattagcg actctaaggc aatccaccat gtcggactca
      901 tgtctgcggc ttcatcaaca attagactat cataacggtc ttgtattttc tcagccgccg
      961 ccatcaacaa ttctggacaa tcgtcattaa aaaaatgtcg gcgttgttgt tcatcagcag
     1021 gtacttgata tgtgagtcct gcctgtaaag cacaaaaacg taccagttca tgaaaatgca
     1081 aaactgttat ttgttgctta taagcggata actgctcggc aagttgtaaa gcgagtgctt
     1141 tgttataaca ggtaaataat atttttttgc cttgttgagc gtgttctcgt gccatcatag
     1201 tggctaataa tgttttgcct gaacctgctc ctccttcaat caataatcgt ttttgtgttc
     1261 ttaacaaacg taaaacacga atttgctgct ctgtggcttg atgtaattgg gtgactgatt
     1321 catcaagttc attagcaagt ggtacaagga gtaagcaatc aggtgccaat agttctttca
     1381 gtgcattttg ctgaatcttt gtccatacag gtgatgatgc ctgcttaact tcagcaaaca
     1441 ttttttctag ttgtggtaaa ggattactta gtgtatgtcg gtcaaagata aagtgtgagc
     1501 taggggcttc ggcaataaca agctgttttg gccattttac atcaggaaac caaacagcat
     1561 gagtgattgc taaatgttcg actgttgttt tacctagccg tttttctagc ttttcaatca
     1621 acgcaaaacg attacgtcgt gcttgactaa aaggtgactg cttcattggc cgaccagaag
     1681 cataaaacca ttgtccatct ttaagataaa cactccctgc cttgacttca aaaataatca
     1741 atccatactg tggattaaaa acaacaacgt cagcctcacc aacttcctca ccataattgc
     1801 caaggttacg ccattccacg gtattaaata tctgccatgg tgatggcaaa ctagataagg
     1861 cagacaatac tttttgctct gcaatatttc ctattgcttt aacttcacta atatcca
//
LOCUS       DAOQMB010000448         1914 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42054_length_1914_cov_7.162453, whole genome shotgun sequence.
ACCESSION   DAOQMB010000448 DAOQMB010000000
VERSION     DAOQMB010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42054_length_1914_cov_7.162453"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="PLY05_10910"
     CDS             <1..232
                     /locus_tag="PLY05_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03848.1"
                     /translation="KPNSDKDNVRHLMILTQWLLRGMAMDKHLVFNERVIEHYVRVWC
                     RVMAGHLHTKPNVDTPPPRPEFWDVSIDKMPR"
     gene            362..1540
                     /locus_tag="PLY05_10915"
     CDS             362..1540
                     /locus_tag="PLY05_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HNC03849.1"
                     /translation="MSTATRLGFEPWLILLGALTAIGPLSIDMYLPSFPILEQELGKG
                     AQLSLASFFMGMTLGQVLYGSLSDRFGRKPPLVIGLSIFVVAALAASQAHTMHELIIY
                     RFFQGFGGCSGMVIARAIVRDRCPPSQVAQAFSMLILVMGLAPILAPLLGSYFLHHFD
                     WHSIFYFKAIFAGLCLVAIMSLIPESLKQPSQLQLKQVLVSYWKLLFDRPFIGYTLSS
                     SLAFSGMFAYIAGSPDLLINLYGLSPSQYSLIFGANALGFIIASQINAWLLRRGYSMF
                     KLLHYALYIPAIASSLLCMSIFLHIESLTLLLIGLFSYIASLGFISPNAGAAAMANQG
                     HQAGTASALMGVLQFSLAAVIGGILGIWQTNTALPIFAILAFCGVSSFTWHRLIVNRR
                     "
     gene            complement(1520..>1914)
                     /locus_tag="PLY05_10920"
     CDS             complement(1520..>1914)
                     /locus_tag="PLY05_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC03850.1"
                     /translation="ANSEALSMAIAQCERELALLPSIEQDMVMMVNILLQADEQGYLT
                     PTQVADRLHISERSLRRHLQQQGTSYQQLLETARQRDAQQLLRQTQAEIQLIAQRLGY
                     QNPANFTRAFKQWTGKTPSEFRLTTIYD"
BASE COUNT          460 a          387 c          399 g          668 t
ORIGIN      
        1 aaaaccaaac tctgacaaag acaatgttcg ccatttgatg attttaaccc aatggttgtt
       61 gcgtggcatg gctatggata aacacttagt gtttaatgaa cgtgtcattg agcattatgt
      121 gcgtgtttgg tgtcgggtca tggcgggtca tttacacacc aaaccgaatg ttgatacacc
      181 gccaccacgt cccgaatttt gggatgtgtc tattgataaa atgccgcgtt aaccatcaaa
      241 aaccttgcta cttttgtaac aaaatatata tttttgccat tatttagcgc atatttacat
      301 acctctcccc tcacactaac tctctcttag cagagagagc attccctttt ttgaggcatt
      361 tatgtccact gcgacgcgct taggttttga gccttggtta attttattgg gtgctttaac
      421 agcgattggc ccattatcta ttgatatgta tttacctagt tttcccattc ttgaacagga
      481 acttggcaag ggtgcacaac tgagtttggc aagttttttt atgggtatga cccttggtca
      541 agtgttatat ggctcgttaa gtgaccgttt tggtcgcaaa ccgcctttag tgattggatt
      601 gagtattttt gttgtggcgg ccttagcggc cagtcaagca cacaccatgc acgaattaat
      661 tatttatcgc ttttttcagg ggtttggtgg ctgctcgggt atggttattg cgcgagcgat
      721 tgttagagat agatgcccac cttcacaagt cgcgcaagct ttttcgatgc tcattttagt
      781 catgggacta gcccccattc ttgcgccatt attaggtagt tactttttac atcattttga
      841 ttggcatagc attttttact ttaaagcgat ttttgcaggt ctttgtcttg tggctattat
      901 gagcttaatt cccgaaagtt taaaacagcc tagtcaatta caacttaaac aagtcttagt
      961 cagttattgg aaactcttat ttgaccgccc gtttattggc tataccctat ctagtagttt
     1021 agctttttcg ggtatgtttg cttatattgc tggctcgcct gatttattaa ttaatttgta
     1081 tggcttatca cccagtcaat atagcttaat ttttggtgca aatgcgcttg gttttattat
     1141 tgccagtcaa attaatgcgt ggttattacg gcgcggctat tcgatgttta agctcttaca
     1201 ctatgcttta tacattcctg ctatcgccag tagtttgttg tgtatgagca tatttttaca
     1261 tatagaatct ttaacgttat tactaatagg tttatttagt tacattgcca gtttgggctt
     1321 tattagtccc aatgcaggcg cggcagcaat ggccaatcaa ggtcatcaag caggcacagc
     1381 gtctgcctta atgggggtgt tgcaatttag tttagcggcg gtgattggcg gtattttagg
     1441 gatttggcaa acaaataccg ctttacccat ttttgctata ttagcctttt gtggtgtcag
     1501 tagttttaca tggcatcgtt taatcgtaaa tcgtcgttaa tcgaaactca ctcggcgttt
     1561 tacccgtcca ttgtttaaat gcacgggtaa aatttgcggg attttgatac cccaaacgct
     1621 gggcaattaa ttgaatttca gcctgtgttt ggcgtaataa ctgttgggca tctcgttgcc
     1681 gtgcagtctc taataactgc tgatagcttg taccttgttg ttgcaaatga cgacgcaaac
     1741 ttcgctccga aatatgcaag cgatccgcca cttgtgttgg tgtcaaatat ccttgctcat
     1801 ccgcttgtaa taagatattc accatcatta ccatatcttg ctcaatactt ggcaataaag
     1861 ccaactctcg ctcacactgt gcaatcgcca tgcttaaagc ttcagaatta gcgg
//
LOCUS       DAOQMB010000449         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42093_length_1913_cov_17.155544, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000449 DAOQMB010000000
VERSION     DAOQMB010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42093_length_1913_cov_17.155544"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1863)
                     /locus_tag="PLY05_10925"
     CDS             complement(<1..1863)
                     /locus_tag="PLY05_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNC03851.1"
                     /translation="MTLAQLPTLGLGISLSLASQPDPVNLVKHPLGASFVEYAGLADV
                     QSVWPQIQRIHQAGAPVLYHPSYINFCGSFANDSAWLQTAAQHIYQVKSAWFAQDCAY
                     CFWQSGASYSTQLGYFLPPILNEVSLQLAIERVQEVQAFMSVPVAIEPPPMTFMVGTM
                     PLMQFFGRLATQTDCAILLDMGHLVSYELASGQRILSQWQDFPAERVIEVHIAGGRIK
                     QTEQGDIYVDAHECAIVEQTWTMLKELLPKLPNLKAVCYECEGVQDEQQILQVLAKIR
                     QLIIEKSKNPELVAVVQGQPFVQQNQEPPLLTSEDWGEGLNLKINNFANQERLLFDLV
                     FNDKVRQLFATQPQKLFANYQLSTQEQADFIGINPHALALDVRVRVDLILSQWCRSLP
                     LTFSLVSSLSNGLALLKQLVDSQTMLQAPNDRIVYFATRLRQQLIAQPQIDLQELSLL
                     VAVLDAELGMASTSRLLKQAVINEQEIATTQSEPFNSQAKITIADYVSASLLPLPYQQ
                     LKQALCPCSGAELWRSLNKQPLSAKQRQQLWTQGEAHLFVAKAKVAMVSLCEPHIEHD
                     TAELAEGFANLLSHVDGSMSVDDILQQLQQIGATPSLLTNIQQGFKLLWERGMIR"
BASE COUNT          561 a          410 c          374 g          568 t
ORIGIN      
        1 tctaatcatc cctctttccc acaacagctt aaatccttgt tgaatattag ttaataacga
       61 tggggttgct ccaatctgct ggagttgttg caatatgtca tcaacactca ttgaaccgtc
      121 aacatggctt aacaaattgg caaaaccttc agccaactca gcagtatcat gctcaatatg
      181 gggttcgcac aacgacacca tcgccacttt tgctttagca acaaataaat gtgcttcacc
      241 ttgtgtccac agttgttgac gttgcttagc tgataacggc tgtttgttta aagatcgcca
      301 cagttcagcg ccagaacaag gacacaatgc ttgtttgagt tgttgatacg gcaagggcaa
      361 caatgacgca ctgacataat ccgcaatggt gattttggct tggctattaa atggctcaga
      421 ttgagtcgtt gctatttctt gctcattgat tactgcttgt tttaataggc gactggtact
      481 cgccatgccc aattctgcat ctaatactgc gactaacaac gataactctt gtaagtctat
      541 ttgaggttgg gcaattaatt gttggcgtaa acgggtagca aaatagacaa tacggtcatt
      601 aggtgcttgc aacatggttt ggctatccac taattgtttt aacaatgcca agccattgct
      661 taatgaagaa accaaactaa aggttaaagg taaactgcga caccattgcg ataaaattaa
      721 atcaacccgc acccgcacat ccaacgccaa agcatgagga ttaattccta taaaatcagc
      781 ttgctcttgt gtgcttaatt gataattggc aaataatttt tgtggttgtg tcgcaaacaa
      841 ctgacgtacc ttgtcattaa atactaaatc aaataatagc cgttcttgat tagcaaaatt
      901 atttattttg agattcagtc cctcacccca atcctctgac gttaagagag gaggttcttg
      961 attttgttga acaaatggct gcccttgcac caccgccacc agctcaggat ttttactttt
     1021 ttcaataatt aactgtctga ttttagctaa tacttgcaaa atctgttgtt catcttgtac
     1081 tccttcacac tcataacaga ctgcttttaa attgggtagt tttggcaata actctttgag
     1141 cattgtccat gtttgttcca caatcgcaca ctcatgggca tcaacataaa tatcaccttg
     1201 ttcggtctgt ttaatacgcc cacctgcgat atgtacttca atcacccgtt cggcaggaaa
     1261 atcctgccat tgcgataaaa tgcgttgacc actggccaat tcataagaca ctaaatgccc
     1321 catatctaat aaaatcgcac agtcagtttg cgtcgccaaa cgaccaaaaa actgcattaa
     1381 tggcattgtg ccgaccataa aagtcatggg tggtggttca atggcaacag gtacagacat
     1441 aaacgcttgt acttcttgca cacgttcaat ggctaattgc aacgatactt catttaaaat
     1501 gggtggcaaa aaatagccta attgtgtgga gtaacttgcg ccactttgcc aaaaacaata
     1561 ggcacaatct tgggcaaacc atgctgattt tacttgatag atatgctgtg cggctgtttg
     1621 cagccatgcg ctgtcattgg caaaagagcc acaaaaatta atataagagg gatgatataa
     1681 aactggcgcg cctgcttgat ggatacgttg tatttgtggc cagacacttt gcacatcggc
     1741 taagccagca tattcaacaa aacttgcccc taaaggatgt ttgactaaat taacagggtc
     1801 aggctgtgag gctagactga ggctaatacc taaaccaagt gtgggaagtt gagctagagt
     1861 catgttttgc gctttgaaag attatttttc gtatcagttt atcattaatg gca
//
LOCUS       DAOQMB010000450         1913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42094_length_1913_cov_16.421421, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000450 DAOQMB010000000
VERSION     DAOQMB010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42094_length_1913_cov_16.421421"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1913)
                     /locus_tag="PLY05_10930"
     CDS             complement(<1..>1913)
                     /locus_tag="PLY05_10930"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HNC03852.1"
                     /translation="PNLMMVFDTSGSMAECVDSADGKTCADVNKKRINVLKTAMKQVL
                     RGDTAAGVSPIPGFVKMGITRYHADSNRGGYVLYPTRPLDAFVEINPNGIVSNIVATA
                     DGDAIQGFNQTTSGTSASAAVKIGVEGSNDYIAGFQFANVRIPRGATVTNAYIEVTPN
                     ANTSGLTIWEVSAEASGNANTYASTQINTRSYGNTTNYSPPTWTNGVVTQIPVTLAIN
                     EVVKRSDWCGGNALSVRIRDIGGVKSLRTAHSFNTAGSDAAKPRLVVNYLVNPDATDS
                     CIKNISKNTVLTVANSNDDAYWAANGSSTTNTTASSLTINNVGSGSVKRENGIRFRNV
                     QIPKNATINSAVLRVTSNNDYSPVANTRIMGFSSPNVDFCPSGTCSTPTVLKDLPVTS
                     NSQVWNPGSVAKNVAYDIDVKNIVQEIVNNSAWDVDKHIGFKLFNDTTTSRNTAIYSF
                     NGSSTRQPRLIIDWTVNELNNLTVLETVRDQLEAEVNALTIPSSTPLAGAMAAATRYM
                     YGLQPHFKEATDYDPRVVVNPGNTSTVRYVSPIPEDDQCSANYIFLLTDGEPNYEADI
                     KNDAKAIMESSTAVCSGSSGAANWDCMKKLATYNNGISKLNGQPKKRIYTSTMILGPL
                     SSAEANMQQVASLG"
BASE COUNT          558 a          422 c          342 g          591 t
ORIGIN      
        1 cctaaagacg ctacttgctg catattcgct tctgcactag acaacggacc taaaatcatc
       61 gtgctggtat aaatacgttt tttaggttgg ccatttaatt tactaatgcc attattatat
      121 gttgccaact tcttcataca atcccagtta gctgctccac tactgcccga gcaaacagca
      181 gtgcttgact ccataatggc tttggcgtca tttttgatgt cagcctcata atttggttca
      241 ccatctgtta acaaaaagat ataattagca gaacattggt catcttcagg aattggactg
      301 acataacgga ctgttgatgt atttccagga ttcaccacaa ctcgaggatc atagtctgta
      361 gcttctttaa aatgcggctg caaaccatac atataacggg ttgctgcagc catagcccct
      421 gccaatggtg ttgaactagg tattgttaag gcatttacct ctgcctctaa ttggtctcga
      481 actgtttcta ataccgttaa attattaagc tcattgaccg tccaatcaat gattaaacgt
      541 ggctgacgtg ttgatgagcc attaaaacta taaatagcag tgtttcgact agttgtggtg
      601 tcattaaaca atttaaaacc aatatgtttg tctacatccc atgctgaatt gttgacaatt
      661 tcttggacga tatttttgac atcaatatcg taggcaacgt tttttgccac agaaccaggg
      721 ttccaaactt gactattact tgttactggc aaatctttta atactgtagg tgttgagcaa
      781 gtacctgaag gacaaaaatc aacattaggc gaactaaacc ccatgattct ggtatttgct
      841 acaggactat aatcattatt tgacgtgact ctaagcaccg cactattgat ggttgcattt
      901 ttgggaattt gtacatttct aaagcgaatg ccgttttctc ttttaacact gccactacca
      961 acattgttaa tggttaaact actcgctgtt gtattggttg tgctagaacc attggcagcc
     1021 caataagcat catcattact atttgccaca gtcaaaacgg tatttttgct aatattttta
     1081 atacaactat cagttgcatc aggatttact aaataattaa ccaccaagcg cggttttgct
     1141 gcgtcactcc ctgccgtatt aaacgaatgt gccgtgcgta atgattttac accaccaata
     1201 tctctaatcc gcaccgataa ggcattacca ccacaccaat cactacgctt gactacctca
     1261 ttaattgcta aggtgacggg tatttgtgta actacaccat ttgtccatgt tggtggagaa
     1321 tagttagtgg tatttccata agagcgggta ttaatttgag tcgatgcata ggtatttgca
     1381 ttacccgaag cttcggcact gacttcccaa atcgtcaaac cactggtatt agcattaggt
     1441 gtgacttcta tataagcatt agtcacggta gcaccacgag gaatgcgcac attggcaaat
     1501 tgaaaacctg caatataatc gttactacct tcaacaccaa ttttgacggc tgccgatgca
     1561 ctagtaccac ttgttgtttg attaaagcct tggatagcat caccatcagc ggtggccact
     1621 atattactta caataccatt tggattaatt tcaacaaacg catctaacgg tctagtcgga
     1681 tataatacat atccgcctct gttagaatca gcatgataac gagtaatacc catttttaca
     1741 aaacctggaa ttggagaaac acctgcggca gtatcaccac gcaacacttg cttcatagcc
     1801 gttttaagga cgttaatacg ttttttattg acatcagcac atgtcttacc atccgcagag
     1861 tcaacacact cagccatact acccgaagta tcaaatacca tcattaaatt agg
//
LOCUS       DAOQMB010000451         1912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42135_length_1912_cov_16.345719, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000451 DAOQMB010000000
VERSION     DAOQMB010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42135_length_1912_cov_16.345719"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1912)
                     /gene="rnr"
                     /locus_tag="PLY05_10935"
     CDS             complement(<1..>1912)
                     /gene="rnr"
                     /locus_tag="PLY05_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HNC03853.1"
                     /translation="SWQDPQAQREAEKYENPIPSRELILSILNQHNKALTAEQLAGVL
                     GLYDDERQFALQRRLGAMIRDGQLSADRRGAYKPLDETECVKGYVQGHPDGFGFLIPS
                     DKSPDILLTSKQMRLVFHGDIALVRVVGFDHKGRPEGRIVRVIEHKTEHLVGRYCEED
                     GMAFVLPDNPRITQEIVLQGDNKGAQLGQFVDVRIVHQPTHHNLAVGEVVEVLGDYMS
                     AGLEIEIALRNFEIPHIWPADVEQQVAGMTPEVAEADKLDRVDLRDLPLVTIDGEDAK
                     DFDDAVFAQKRAGGGYRLVVAIADVSHYVKPYEPLDMEARQRTTSVYFPGYVVPMLPE
                     ILSNGLCSLKPHVDRLCMVCDMTISRLGRVTSYRFYEAVMHSQARLTYTQVSAVLQQP
                     DSELAQQVSNTYPHLVQPLHNLYGLFKILRANREKRGALDFEAPETYIVFDEQRKIDK
                     ILPRTRNDAHMLIEECMLAANVCAAEYVQKRQLPALYRNHEGPKDEKLLKLKSYLSLL
                     GIDFKAKKATPADFQAVLLQIKDRPDAAIIETMLLRSMMQAVYSPDNLGHFGLAYEAY
                     AHFTSPIRRYPDLLLHRVIRHSLRHPDAQPAIEMTEMLALGEKCSLNERRADEATRDV
                     TAWLKCEYMQDH"
BASE COUNT          555 a          449 c          385 g          523 t
ORIGIN      
        1 atatggtctt gcatgtattc acattttaac catgcagtga catcacgcgt ggcttcatca
       61 gcacgacgtt cgtttaagga acatttctcg cctaaagcta acatttcggt catctcaata
      121 gcaggttgtg catcaggatg acgtaagcta tgacgaatca cacgatgtaa taacaagtca
      181 ggataacgac gaatcggcga agtaaaatgg gcataagcct catacgccaa accaaaatgg
      241 ccaagattat ctgggctata aaccgcttgc atcattgaac gcaagagcat ggtttcgata
      301 atcgcggcat caggacggtc tttgatttgt agtaataccg cttgaaagtc ggctggtgtt
      361 gctttttttg ctttaaaatc aattcccaac aacgacaaat aactctttaa tttaagcagt
      421 ttttcgtctt ttggcccttc atgattacga tacaaagcag gcaactgacg cttttgtaca
      481 tattcagcgg cacacacatt ggctgccaac atacactctt caattaacat atgagcgtca
      541 ttgcgggtac gtggcaaaat tttgtcaatt ttacgttgtt catcaaagac gatatacgtt
      601 tcgggtgctt caaaatctaa tgcaccacgt ttttcacggt tagcgcgtaa aatcttaaac
      661 aggccataca aattatgtaa aggttgaacc aaatgcggat aggtgttgct aacttgctga
      721 gccaactcac tatcaggctg ttgtaacaca gcactgactt gggtgtacgt taatcgtgct
      781 tgtgagtgca tcaccgcttc ataaaagcga taactggtga ctcgccctaa acgggaaatg
      841 gtcatatcac agaccataca caaacgatca acgtgtggtt ttaacgaaca taaaccgttc
      901 gataaaattt cgggcaacat tggcacaaca taacctggaa aatacactga ggttgtccgt
      961 tgtcgtgctt ccatgtctaa gggctcatag ggtttgacgt agtgtgatac atcagcaatg
     1021 gccaccacta aacgataacc accccctgct cgcttttggg caaataccgc atcatcaaaa
     1081 tctttggcat cttcaccatc aatcgtgact aaaggtaaat cgcgcaagtc aacacgatca
     1141 agtttgtcgg cttcggccac ttctggcgtc attcctgcca cttgctgctc aacatcggct
     1201 ggccaaatat gcggaatttc aaaattacgc aaggcgattt cgatttctaa gcctgcggac
     1261 atatagtcac ccaagacctc aaccacttca cccacggcta aattgtggtg agtgggttga
     1321 tgcacaatgc gcacatccac aaattggcct aattgcgcgc ctttgttatc accttgcaaa
     1381 acaatttctt gggtaatacg tggattatca ggcaagacaa atgccatgcc atcttcttca
     1441 caataacgtc cgactaaatg ttcggtttta tgctcaatca cacgcacaat ccgaccttca
     1501 ggacgacctt tgtggtcaaa tccaaccaca cgcactaagg caatgtcacc atgaaacacc
     1561 aatctcatct gtttggaggt taataaaata tcaggcgatt tatcactagg aattaaaaag
     1621 ccaaaaccat caggatgccc ttggacataa ccttttacac attcagtttc atcaaggggt
     1681 ttatacgcac cgcgtctatc ggcactgagt tgaccatcac gaatcattgc acctaagcga
     1741 cgttgtaagg caaattggcg ttcatcatca tacaaaccta atacgcctgc taactgttcg
     1801 gcagttaagg ctttgttatg ctgattaaga atactgagga taagttcacg acttggaata
     1861 ggattttcgt acttttcagc ttcacgctga gcttgtgggt cttgccacga at
//
LOCUS       DAOQMB010000452         1910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42215_length_1910_cov_6.561725, whole genome shotgun sequence.
ACCESSION   DAOQMB010000452 DAOQMB010000000
VERSION     DAOQMB010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42215_length_1910_cov_6.561725"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..588
                     /locus_tag="PLY05_10940"
     CDS             82..588
                     /locus_tag="PLY05_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC03854.1"
                     /translation="MAIYRRLYCHGGCYFFTVNLANRHSQLLIEHIELLKQSIRHVQQ
                     KHPFYIDAIVVLPEHLHCVWTLPTNDMDYSTRWRLIKSYFSYHLPYLNDEVYSDSRVK
                     KSERGIWQRRFWEHLIRNEEDYRAHIDYVHINPVKHQLVNRVVDWPYSSFHKAVKQGI
                     YDVNWAGL"
     gene            698..1630
                     /locus_tag="PLY05_10945"
     CDS             698..1630
                     /locus_tag="PLY05_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03855.1"
                     /translation="MQCDLTYNFDGLGNQLVAVLSGEVDFPQFVIAYVALAYLQTQQG
                     LHIELTICQKDETGKINTNHKCCIALYCPIANIEQLTPISEQQSPWQGCYGEVVAPEN
                     YQHYALAQQALANAVTVIKADSYLWEYVQTGKIQKLEQQNLSLAYYHSPQNPWPFEYV
                     ILGLQVLALPFSQFKAYIPNQNLTPIIFEIDGAIQSNNLMSVILSGVISCINMGKSWN
                     EWIDENVLIDQNIEQAFTQLYIQLTQSSLNFDEQNEDSLEQLLAPCRQQANHLLTRLG
                     WPLEARQPIKTASELLDEYSYGYYSEMMQLGSVG"
     gene            complement(1684..>1910)
                     /locus_tag="PLY05_10950"
     CDS             complement(1684..>1910)
                     /locus_tag="PLY05_10950"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005190886.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNC03856.1"
                     /translation="MPYLDIIREVKDTYQAPTFAYQVSGEYAMHMAAISNGWLKRDEI
                     ILESLMCIKRSGADAILTYFAKEAAKLLQK"
BASE COUNT          598 a          341 c          378 g          593 t
ORIGIN      
        1 gtgatatgga gcgttaattg aacagtcgtg ggtttcgttc ctcaacccac gctacgataa
       61 tttttaaaag gatttagaga tatggcaatt tatcgccgat tatactgtca tggcggatgt
      121 tactttttta cggttaattt agcgaatagg catagccaat tattaattga acatattgag
      181 ttactcaaac aatccattcg ccatgttcaa caaaaacacc cattttacat tgatgcaata
      241 gttgttttgc ctgagcattt acattgtgta tggacattgc cgacaaacga tatggactat
      301 tcaacccgtt ggcgtttgat taaaagttat ttctcatatc atttaccgta tttgaatgat
      361 gaagtttatt cagatagtcg tgttaaaaag tccgaaagag gcatttggca acgccgcttt
      421 tgggaacatt taattagaaa tgaagaggat tatcgcgctc atatagatta tgtgcatatt
      481 aaccctgtaa aacatcagtt ggttaatcgt gttgttgatt ggccatattc atcatttcac
      541 aaagccgtta aacagggtat atatgatgtt aattgggcag gattgtagat tgtagtgtgg
      601 gttgaggaac gaaacccaca atataacaat gaccgtgggt ttcctacgtc aacccacgct
      661 acaattgcct atcataagaa attaatggag tatgccgatg caatgtgatt tgacttacaa
      721 ctttgatgga ttgggaaatc aattagtcgc tgttttatct ggcgaggtag attttccaca
      781 attcgttatt gcttatgtcg cgcttgctta tttgcagact cagcaaggtt tgcacataga
      841 gttaacgatt tgccaaaaag atgaaacagg taaaataaat accaatcata aatgttgtat
      901 tgctttatat tgcccaattg ctaatattga gcagttaaca ccaatttcag agcagcaatc
      961 tccgtggcag ggatgttatg gtgaagtggt tgcccctgaa aattatcaac attatgcctt
     1021 agctcaacaa gccttagcta atgctgtgac tgtgataaaa gctgattcct atttgtggga
     1081 atatgtgcaa acaggaaaaa ttcaaaaatt agaacaacaa aatttaagct tggcttatta
     1141 tcattcacct caaaatcctt ggccgtttga atatgtgatt ttgggcttgc aggttttagc
     1201 tttgccattc tcacaattta aagcgtacat cccaaatcaa aacctaactc ctataatttt
     1261 tgaaattgat ggtgcaatac aaagtaataa tcttatgtcg gtgattctgt caggcgtgat
     1321 ttcttgtatc aatatgggaa aaagttggaa tgagtggata gatgaaaatg tgttaattga
     1381 tcaaaatata gaacaggcat ttacacagtt atatatacaa ctcactcaat catcgttaaa
     1441 ttttgatgag cagaatgagg acagtttaga gcaactttta gcaccatgtc ggcaacaagc
     1501 taatcatcta ttaactcgtt taggttggcc attagaggct cgtcaaccaa ttaaaacggc
     1561 aagcgagtta ttggatgaat atagctatgg ttattattca gaaatgatgc agcttggtag
     1621 tgtgggttga gaaacgaaac ccacaatata acaatgaccg tgggtttcct acgtcaaccc
     1681 acgctacttc tgcaacaact tcgcagcctc tttggcaaaa taggtcaaaa tcgcatctgc
     1741 acccgagcgt ttaatacaca ttaacgactc taaaataatt tcatcacgtt tgagccaacc
     1801 attactaatc gcggccatgt gcatggcata ttcaccactg acctgataag caaacgtggg
     1861 tgcttgatag gtatctttta cttcacgaat aatatccaaa tagggcatgg
//
LOCUS       DAOQMB010000453         1903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42534_length_1903_cov_6.848485, whole genome shotgun sequence.
ACCESSION   DAOQMB010000453 DAOQMB010000000
VERSION     DAOQMB010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42534_length_1903_cov_6.848485"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..960
                     /locus_tag="PLY05_10955"
     CDS             118..960
                     /locus_tag="PLY05_10955"
                     /inference="COORDINATES: protein motif:HMM:NF036707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03857.1"
                     /translation="MIPIKKYSDRYLGWSPADLQSYDYGKYWQPLLKPLSVEAQQALL
                     QGALPEILLPDLAHAPQALFGYPDYPLENGYCLCADGATHIAIRTDMPNVSPKMIDWW
                     FAWHNGGAERYKLWHPQAHVHAQWLGSVNTKLRGRALYIGQTSCVDEYVGGQLGRYAI
                     HFLDPKQLGFNNPSLADDQQATAICARVGFADYPINIGYLVHHVRQTATGAEMRSRFW
                     LGGRYAQARQDNRFTQLLVTGAKQLLKPSEKDAKDLLVHCAQEMAHLASFLPNLYADY
                     QQLD"
     gene            complement(957..1517)
                     /locus_tag="PLY05_10960"
     CDS             complement(957..1517)
                     /locus_tag="PLY05_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC03858.1"
                     /translation="MRYSAKHKAETRQKLIEKAGSLAKKDGFATTGVDGLMSTVGLTG
                     GAFYSHFSSKNELLLEIIRQELKKSQDLLETNLSNHNIEPFLNLYLSQLHLNFPALGC
                     ALPSLTTEVGRADAEVKQAFSEGLAQIHQILNTQLSNPQMAWATIAMTVGAVNLARAL
                     PAGEQQQALLTACQNMILAMAKPTIS"
     gene            1694..>1903
                     /locus_tag="PLY05_10965"
     CDS             1694..>1903
                     /locus_tag="PLY05_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNC03859.1"
                     /translation="MAFVVLEECIQCKHTDCVAVCPVDCFYEGPNFLVINPDECIDCA
                     LCEPECPVDAIRAEEEVPDEQKHFIA"
BASE COUNT          550 a          345 c          396 g          612 t
ORIGIN      
        1 atatgaccaa ttgcaaatac tacacgctgc gtaacatcag ttacaaaaac tagatcagat
       61 tcgagctaaa tatgatcctg ataatcgttt tcatccgtgg atggggagag tgacaccatg
      121 ataccaatca aaaaatattc tgatcgttac ttggggtgga gtccggctga tttacaaagt
      181 tatgactatg gcaaatattg gcagccttta ctcaaacctt tgtcagtaga ggcgcaacag
      241 gcattattac aaggggcatt acctgagata ttattgcccg atttagccca tgcaccacaa
      301 gctttgtttg gttatcctga ttatccgctt gaaaatggtt attgtctgtg tgcagatggt
      361 gcgacacaca ttgccattcg gacagatatg cctaatgtta gtccaaagat gatagattgg
      421 tggtttgctt ggcataatgg cggtgcagag cgttacaaat tatggcatcc acaagcacat
      481 gttcatgcac agtggcttgg ttcagtgaat actaaattac gtggtagagc tttatatata
      541 ggtcaaacgt cgtgtgttga tgaatatgtt ggtgggcagt taggtcgtta tgccattcat
      601 tttcttgatc ctaaacaatt aggttttaat aatccttctt tagctgatga ccaacaagca
      661 accgctattt gtgcaagagt aggttttgcc gattatccaa ttaatattgg ttatttagtg
      721 catcatgtgc ggcaaacagc aacaggtgca gaaatgcgct cacgtttttg gttgggtggc
      781 cgttatgcac aagctcgtca agataatcgt tttactcaat tattggtgac gggagctaaa
      841 caattattaa agccaagtga aaaagatgcc aaagatttat tagtgcattg tgcccaagaa
      901 atggcacatt tggcgagttt tttacccaat ttatatgccg actatcaaca gctagattag
      961 cttatagttg gcttagccat tgctaaaatc atattttggc acgctgttaa taacgcctgt
     1021 tgttgctcgc cagcaggcaa agcacgcgcc aaattgacag cacccaccgt catggcaatg
     1081 gttgcccatg ccatttgtgg attgctaagt tgtgtgttta agatttgatg aatttgcgcc
     1141 aagccctccg agaaagcttg tttaacctct gcatcagcac gtcccacttc ggtagttaaa
     1201 ctgggtaaag cacaacccaa agcaggaaag ttaaggtgta attgactaag atataaattt
     1261 aaaaaaggtt caatattatg attagacaag ttggtttcta acaaatcttg tgattttttt
     1321 aattcttgac gaattatttc taataacagc tcatttttag acgaaaaatg actataaaaa
     1381 gctccacctg ttaagcctac agttgacatc aaaccatcaa caccagtagt ggcaaaaccg
     1441 tctttttttg ctaatgaacc tgctttttca atcaattttt gtcgagtttc ggctttatgt
     1501 ttcgcagaat aacgcatgat ttgtctctaa ctttttaatt attttagctg acgatcgttt
     1561 actaataaat gtttattggc tgtaaatcga ctaattttat ctttttcagc caattgacaa
     1621 gctatatttt ataaattaac gtccgtttag taaacggtcg tttagtttaa tagcataaaa
     1681 taaaggtgtc atcatggcgt ttgtggtgtt agaagagtgt attcaatgca aacacaccga
     1741 ttgtgtagca gtctgtcctg tcgattgttt ttatgaaggg ccaaattttt tggtgattaa
     1801 tcctgatgag tgtattgatt gtgccttatg cgagcctgaa tgtcctgttg atgcaattcg
     1861 tgccgaagaa gaagtgcctg atgagcaaaa acattttatt gcc
//
LOCUS       DAOQMB010000454         1901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42623_length_1901_cov_17.621343, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000454 DAOQMB010000000
VERSION     DAOQMB010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42623_length_1901_cov_17.621343"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLY05_10970"
     CDS             complement(<1..226)
                     /locus_tag="PLY05_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03860.1"
                     /translation="MSATKQLILANGFIAICMDSLWLRFKLSVLLTFKYGFYREGQWS
                     ATEADDEIYAHFVRENLRILAGWDRRLGHYL"
     gene            complement(344..1258)
                     /locus_tag="PLY05_10975"
     CDS             complement(344..1258)
                     /locus_tag="PLY05_10975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC03861.1"
                     /translation="MEEPPPTPIISRYLLLIFIWSTTPLAVVLSIKDLHPIWALACRF
                     ALAVPLSALCLKLLGEHLPLNKAAIRSYVAGMLSLFGAMIFTYLGAAYLPSSLISLLF
                     GLSPLMVGLLAHGVFKSQLLRIEQWLGLVVAFLGLMIIFSHGDQGQTQALGIVFILLG
                     VSCYVASVFWVKHENANLHPLAQTTGSLVLSTVAMFLVLPFYWQDKPTHLPSAISLWA
                     LLYSATMATLVAMFCYFYLIKKLAPATVSLTTFITPVLALVWGFWLNGERFSTFTAIG
                     MAVIMLGMSAYFLQDIKQIFRTYAFHRN"
     gene            complement(1462..>1901)
                     /locus_tag="PLY05_10980"
     CDS             complement(1462..>1901)
                     /locus_tag="PLY05_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC03862.1"
                     /translation="PHRVPIIVECLTDNVNRTISSMRVLFRKGQLGASGSVAWDFDHL
                     GMIEASPAKADADAELAAIEAGADDFEVDEDGEVLFYTAMADLDLVCRELPNQGFTVN
                     SAKLGYRAKNPVSLSDAEREEVEAFLEAMDSDDDVQHVYVGLA"
BASE COUNT          647 a          435 c          321 g          498 t
ORIGIN      
        1 ataaataatg gcctaaacgc cgatcccatc ctgctaaaat tcgcaagttt tcacgaacaa
       61 aatgggcgta aatttcgtca tccgcttctg ttgctgacca ttgaccttca cgataaaaac
      121 cgtatttaaa agtcaacaat acactcagct taaaacgcaa ccacaacgag tccatacaaa
      181 tagcaataaa accattggcc aatatgagtt gtttagtggc tgacataatt gtgttatccg
      241 tttttttaag gctcaccctt aactattagc aaacaattaa gggtaagtct agtctaattt
      301 tttagccata ttatgactta cgcgattgtc gcttatttgt gcgctaattt cggtgaaatg
      361 cgtaagttct gaatatctgt ttaatgtctt gcaaaaagta agcagacatt cccaacataa
      421 tcaccgccat tcctattgcg gtaaatgtgc taaaacgctc gccatttaac caaaagcccc
      481 atactaatgc taaaacaggg gtaataaagg tggttaatga aaccgttgca ggggctaatt
      541 ttttaattaa ataaaaatag caaaacatcg ccactaaggt tgccatagtt gcactataca
      601 ataatgccca taaactgatg gcactaggta aatgtgtagg tttatcctgc caataaaaag
      661 gcaataccaa aaacatcgct acggttgaca acaccaatga acctgtcgtt tgcgctaaag
      721 gatgtaaatt ggcattttca tgcttgaccc aaaataccga agccacataa caactcacgc
      781 ccaataaaat aaatacaata cccaacgctt gtgtttgacc ttgatcgcca tgactaaaaa
      841 taatcatcaa acctaaaaat gcaacgacca accctaacca ttgctctatt cttaacaact
      901 ggcttttaaa cacaccatgc gccaataaac ccaccattaa aggtgacaaa ccaaataata
      961 aagaaatcaa actcgacggc aaataagccg cgcccaaata agtaaaaatc attgcgccaa
     1021 ataaacttaa catacctgcc acataactgc gtattgccgc tttgtttaat ggcaaatgct
     1081 cgcctaataa ttttaaacac aaggcggata atggcacagc taaagcaaaa cgacaggcta
     1141 atgcccaaat tggatgcaag tctttaatgc tcaataccac cgctaaaggc gtggttgacc
     1201 aaataaaaat taataataaa taacgactta ttatgggtgt cggtgggggt tcttccattt
     1261 gtcagctcca aaaaaaaggc cgctaacagc ggcctttgag tggggattct tttactttat
     1321 ctgtgatttt tgccgccaaa atacagacga gtgacaccat accgcagctc gcagtacatt
     1381 tttcactcgg acggaaatca ataataaagt gttcatagtg tcccgatagt ggctaacaat
     1441 attttattag tcaagtgaat tttaggctaa accaacataa acatgctgaa catcatcatc
     1501 agaatccatt gcttctaaaa atgcttcaac ctcttcgcgt tcggcatcac ttaaagaaac
     1561 aggatttttg gcacgataac ccaacttagc cgagtttaca gtaaaacctt gattgggcaa
     1621 ttcacgacaa acaaggtcta aatcagccat tgccgtataa aataatactt cgccgtcttc
     1681 atcgacttca aagtcatctg cacctgcttc aatcgctgcc aactctgcat ccgcatccgc
     1741 tttggcagga gacgcttcaa tcatacctaa atgatcgaag tcccaagcga ctgaacccga
     1801 tgcacccaac tgccctttac gaaataaaac acgcatactc gaaatagtgc ggtttacatt
     1861 atcggttaaa cattcaacaa taattggtac acgatggggg g
//
LOCUS       DAOQMB010000455         1900 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42666_length_1900_cov_12.358808, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000455 DAOQMB010000000
VERSION     DAOQMB010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1900
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42666_length_1900_cov_12.358808"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PLY05_10985"
     CDS             <1..1038
                     /locus_tag="PLY05_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03863.1"
                     /translation="GKTMSAVVALSSWPINAETPMANAPSIELPNGYIGIPQHYLRYQ
                     QTLSSVRALLADIQFDDKTLLFAGQDPNGLYIQVGMIGRENYERGDQQRPHKLVYGRK
                     WRIDRDTPTSEVIQTAFLAIGKAYEHEVRELLTVKDAWQAKVSAPFSCHQDLPLLTEN
                     TDLVAAEEQPSLQTLADIEQLLAGVKFAQHDIDVVSFHAQQPFSYLVVRLGNAPLARQ
                     QEGDLAHFNQQLVSVTLQENTASEVLFALLDSFLHINQRWVEETFAYQGFARFSRSLD
                     PRQIAAVSVATRPYRRDARNEHFSRGFRQANYDVDRSRVPSLGTGFLAKRNREVIQAY
                     QGLLGHMPEGL"
     gene            complement(1047..1844)
                     /locus_tag="PLY05_10990"
     CDS             complement(1047..1844)
                     /locus_tag="PLY05_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HNC03864.1"
                     /translation="MDFFPQRIVCLTEETTELLYLLGQQHRIVGISGFTVRPPQARKE
                     KPKVSAFTSAKIDKILALKPDLVLGFSDLQADIAAELIKQGVNVYIFNQRSVNEILAM
                     CVQLGAMVGQQQAALTWVNERQAYINQLKQQYRQRTIKPVVYFEEWYDPIITSIQWVA
                     ELIEIAGGQYAFPEHARQSLAKNRIVADHQQVIAKQPDIIIGSWCGRKFRPEQVAARE
                     GWEHLPAVQNQQIFEIKSADILQPGPAALTDGLAQLVEIVEGWYRKY"
BASE COUNT          512 a          393 c          418 g          577 t
ORIGIN      
        1 ggtaaaacca tgagtgctgt tgttgcttta tcgtcatggc caattaatgc tgaaacgcca
       61 atggccaatg cgccaagtat tgaattgccc aatggttata ttggcattcc tcagcattat
      121 ttacgttatc aacaaaccct aagctctgtg cgtgctttac tcgctgatat tcagtttgac
      181 gataaaacct tgttgtttgc agggcaagac cctaatggtt tgtatattca agtgggcatg
      241 attggccgtg aaaattatga gcgtggcgat caacagcgcc cacacaaatt ggtttatggt
      301 cgtaaatggc gtattgaccg tgacacgcca acctccgaag tcattcaaac ggcatttttg
      361 gcgattggca aagcctatga gcatgaagta cgagaattat taaccgtaaa agatgcttgg
      421 caagcaaagg tcagtgcgcc atttagctgt catcaagatt tgcctttatt gacagaaaat
      481 actgatttgg ttgcagcaga agagcagcct agtttgcaaa ctttggccga tattgagcag
      541 ttattggcag gtgtaaagtt tgctcagcat gacattgacg tggtgagttt tcatgctcaa
      601 cagccattta gttatttagt ggtacgtttg ggcaatgcac ctttggcacg tcagcaagaa
      661 ggggatttag cccattttaa tcaacaattg gtgtcagtga ctttgcagga gaataccgct
      721 tctgaggtct tgtttgcttt gttagatagc tttttgcata ttaatcaacg ttgggttgag
      781 gagacttttg cttatcaagg ttttgcacgt tttagtcgta gccttgatcc acgtcaaatt
      841 gcggcggtgt ctgttgcgac tcgtccttat cgtcgtgatg caaggaatga acattttagc
      901 cgtggtttcc gtcaggccaa ttatgatgtt gatcgttcac gtgtgccaag cttgggaacg
      961 ggctttttag ccaaacgtaa tcgtgaggtt attcaagctt atcaaggttt gttagggcat
     1021 atgcctgagg ggttgtaagc taaatattag tattttctat accaaccctc aacaatttcc
     1081 accaattgcg ccaaaccatc ggttaacgcc gcagggccgg gttgcaaaat atctgccgat
     1141 ttaatttcaa aaatttgttg gttttgtacc gctggcaaat gttcccagcc ttcgcgtgca
     1201 gccacttgtt caggtctaaa ttttcgccca caccaagagc caataataat atcgggttgt
     1261 ttagcaatca cttgttgatg gtcagccaca atgcgatttt ttgctaaaga ctgccgagcg
     1321 tgttcaggaa aagcatactg cccacctgca atttcaatta actcagccac ccattgaata
     1381 ctggtaataa tcgggtcata ccactcttca aaatacacaa ctggtttaat tgttcgttgt
     1441 ctatattgtt gttttaattg attaatatat gcctgacgct cgttaaccca cgtcaacgcc
     1501 gcttgttgtt ggccaaccat tgcccctaat tgcacacaca tcgccaaaat ttcgttcact
     1561 gagcgttgat taaaaatata aacgttcact ccttgtttaa tcaattcagc cgcaatatcg
     1621 gcttgcaagt ccgaaaaccc caataccaaa tcgggcttga gtgccaaaat tttgtcaatt
     1681 ttggccgagg taaatgccga gactttgggc ttttctttgc gtgcttgtgg tggacggacg
     1741 gtaaaccccg aaatacccac aatacgatgt tgttgaccta gcaaatacaa aagctcagtg
     1801 gtttcttcgg ttaaacagac aatacgttgg gggaaaaagt ccataaagat attgctctaa
     1861 aaattgtggt ataactataa aaagaaattt taacttagga
//
LOCUS       DAOQMB010000456         1897 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42795_length_1897_cov_16.615635, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000456 DAOQMB010000000
VERSION     DAOQMB010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42795_length_1897_cov_16.615635"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLY05_10995"
     CDS             complement(<1..195)
                     /locus_tag="PLY05_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005178815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HNC03865.1"
                     /translation="MFYRHLSLTGLLLLSSLATALPNDREQPIALAADNATFNEKTGI
                     AIYTGNVEIKQGSLLIKAEQI"
     gene            complement(176..736)
                     /gene="lptC"
                     /locus_tag="PLY05_11000"
     CDS             complement(176..736)
                     /gene="lptC"
                     /locus_tag="PLY05_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNC03866.1"
                     /translation="MAIQNNVSLAGGLFILCVAGYYWGGFGKDAPILLEEADNLPEYQ
                     VSQITGIQINEQGQIVRQITAKQLQHFAEQDETIIHAPIVMLYQQGQKTWQISATKAI
                     SRQDNQWLNLQNQVVAQQLTETALRLETSSLIAEPQKHLLYTQTPVVVRSPNGEIRSL
                     GLQANTEKGTINFIGQVRGTYVLPTP"
     gene            complement(740..1270)
                     /gene="kdsC"
                     /locus_tag="PLY05_11005"
     CDS             complement(740..1270)
                     /gene="kdsC"
                     /locus_tag="PLY05_11005"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HNC03867.1"
                     /translation="MAAVRITEKARAVRLLVLDVDGVLTDGRLYFSEDGQELKTFDTQ
                     DGHGIKMLQQAGIQCAIITGRNTQLVARRAANLGIKHLLQGREDKGVALSALSQELAI
                     PLDEIAYVGDDWPDLPAIRHAGLGIAVANAHKEVRRHADFVTTARGGRGAVREVCDLI
                     LEAQGLYEEALAPYLD"
     gene            complement(1271..>1897)
                     /locus_tag="PLY05_11010"
     CDS             complement(1271..>1897)
                     /locus_tag="PLY05_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNC03868.1"
                     /translation="LHIPLISITANGQSSLAQFANISLEIGKSVEACPLGLAPTSSTT
                     ATLVLGDALAVALLAARGFTAEDFALSHPGGALGRKLLLRVADLMHSGDNVPIVQEQT
                     LIRDALLEISRKGLGMTAVVNQQGQLTGIYTDGDLRRTLDLEVDIRTAPIVQVMSKNP
                     KTIRQHLLAAEAVALMEEKKINGVIVVDEQHKPIGALNMHDLLKAGVV"
BASE COUNT          527 a          403 c          404 g          563 t
ORIGIN      
        1 aatttgttct gctttaatga gcagtgaacc ttgtttgatt tcgacattac ctgtgtaaat
       61 ggcaatacct gttttttcgt taaaagtggc attatctgcg gcgagtgcaa tgggttgttc
      121 tctatcgtta ggtagtgcag ttgccaaact acttaacagt aataagcctg ttaaactaag
      181 gtgtcggtaa aacataagtg cctctgactt ggcctataaa attaattgtg cctttttctg
      241 tattagcttg taagcctaaa ctacgaattt caccatttgg cgaacgcacg acaacaggtg
      301 tttgggtata aagtaaatgt ttctgaggct cggcaattag tgaagatgtt tctaagcgca
      361 gagctgtttc agttaattgt tgagcaacca cttggttttg taaattcagc cattgattat
      421 cttgacgact aatagcttta gttgctgaga tttgccatgt tttttgacct tgttgataga
      481 gcatgacgat gggagcatgg ataatagttt cgtcttgctc tgcaaagtgt tgtaattgtt
      541 tggcagttat ctgtctaaca atttgacctt gttcattgat ttgtatgcca gtgatttgag
      601 agacttggta ttcaggtaaa ttatcggcct cttctaggag aattggggcg tctttgccaa
      661 atccacccca ataatatcct gcgacacaca aaataaataa accgccagct aagctaacat
      721 tgttttgaat agccacagct taatccaaat aaggtgcaag ggcttcttcg tataagcctt
      781 gagcttctaa aattaaatca cacacttcac gcaccgcacc acgaccacca cgcgcagtgg
      841 tgacaaaatc ggcatgacgg cggacttctt tgtgggcatt cgcgaccgca atgcctaagc
      901 cagcatggcg aatcgcaggt aaatcgggcc aatcatcacc aacataagca atttcatcta
      961 aaggaatggc taattcttgg cttaatgcgc tgagtgccac gcctttatct tcacgacctt
     1021 gcaataaatg tttaatgcct aaattggcag cacgtctggc gactaattgg gtgtttcggc
     1081 cagtaataat ggcacattga atgcctgctt gttgcaacat tttgatgcca tgaccgtctt
     1141 gtgtatcaaa ggtttttaac tcttggccat cttcgctaaa atataaacgg ccatcagtta
     1201 aaacaccgtc tacgtctaac actaataaac gaacggcacg ggctttttcg gtaatacgga
     1261 cagcagccat ttaaacaact ccagctttga gtaaatcgtg catgtttaat gcgccaatcg
     1321 gtttgtgttg ttcgtccacc acaatcacac cattgatttt tttctcttcc attaaggcaa
     1381 cagcttcagc cgctaacaaa tgttgacgaa tggttttagg atttttgctc atcacttgta
     1441 caatcggtgc ggtacgaata tccacttcta aatcaagggt acgacgtaag tcaccatcgg
     1501 tataaattcc cgtcagttga ccttgttggt taacaacagc cgtcatgcct agacctttgc
     1561 ggctaatttc taatagggca tcacgaatca gggtttgttc ttggacaatt ggcacattat
     1621 cgccactgtg cattaaatca gcaacacgca ataacagctt tctgcctaaa gctccaccgg
     1681 gatgggaaag ggcaaaatct tcagcggtaa aaccacgagc agcgagtaag gcaacggcta
     1741 aagcatcacc taatactaag gtggcggtgg tgctagaggt tggcgctaaa cctaatgggc
     1801 aggcttcgac acttttgcca atttctaaac taatattggc aaactgtgcc aaactggatt
     1861 gaccattggc agtaatgcta attaatggaa tatgtaa
//
LOCUS       DAOQMB010000457         1894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_42937_length_1894_cov_6.401849, whole genome shotgun sequence.
ACCESSION   DAOQMB010000457 DAOQMB010000000
VERSION     DAOQMB010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42937_length_1894_cov_6.401849"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1894
                     /locus_tag="PLY05_11015"
     CDS             <1..>1894
                     /locus_tag="PLY05_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03869.1"
                     /translation="PLQINQFADKQVKSRLQTLDGVADIRIGGERKPAMRIWLDKEKL
                     ARYSLTPQDVETALRAQNVEIPSGRVESVQREFSVLAETDLNTVQQFENIIIKQQNGL
                     LLRVADVAKVEIAPEDVRRIARFNGKPAVALGVIKTSTANPLEVSKAVRAELPRIEQN
                     LPQGMKVELAYDSSVFIQQSIDSVQKTIIEAVVLVVLVIFFFLRSLRATLIPVITIPV
                     SLIGALIFMNILGFSINTLTLLAMVLAIGLVVDDAIVVLENIYRHIEEGKTPTQAALQ
                     GSKEIAFAVLAMTFTLAAVFAPIAFTEGRTGKLFAEFALTLAAAVIVSGFCALTLSPM
                     MSSKLLKAHSGQENIIYRVIETFLLALTRGYQQLLTIVLKLRWLMVLIAVGASAYMWL
                     VFKGLPSELSPTEDRGVIVSMGIAPEGVTTNYLDKYAQQLETIYSQVPERASTFLIIG
                     MPDETRFMSFNRFIPWEQRERSTQDIAPDLRPKLAGIAGVNALATLPPSLGQGTSSQP
                     IDFVLQTTGSYADLGKMIGQLMEKINQNEGFANPIPDLKLNKPQLSVQVDRDKAALLG
                     VGVSDIGRTLETLLGSRKVTTFKLDGDQYDVILQLADEERRTPEQLDQIYVRNQQGEV
                     LPLASVL"
BASE COUNT          550 a          353 c          419 g          572 t
ORIGIN      
        1 cccttacaaa tcaatcaatt tgccgataaa caagttaaaa gccgtttaca aacgctagat
       61 ggtgttgccg atattagaat tggcggcgaa cgtaaacctg cgatgcgcat ttggttagat
      121 aaagaaaaat tagcaagata tagtttaaca ccacaagatg ttgaaaccgc tttacgcgca
      181 caaaatgtcg aaatcccttc aggtcgagtt gaaagtgtac aacgtgaatt ttcggtatta
      241 gccgaaaccg atttaaatac cgtacaacag tttgaaaata ttattattaa acaacaaaat
      301 ggcttgttat tacgtgtggc tgatgtggcc aaagtcgaaa ttgcgcccga agatgtgcgg
      361 cgtattgccc gctttaatgg taaacctgcg gttgccttgg gagtcattaa aacctctacc
      421 gcgaatcctt tagaagtgtc caaagcagta cgtgccgagt tgccaagaat tgaacaaaat
      481 ttaccgcaag gcatgaaagt tgaattagct tatgattcct cagtgtttat tcaacagtcg
      541 atagattcgg tacaaaaaac cattattgaa gcggttgtgt tagtggtttt agtgattttc
      601 ttctttttaa gaagtttacg tgccacttta attcctgtga ttaccattcc tgtgtcatta
      661 attggcgcat tgatttttat gaatatcttg ggcttttcga ttaatacctt aactttattg
      721 gcgatggtat tagcgattgg tttagtggtt gatgatgcca ttgtcgtgtt agaaaacatt
      781 tatcggcata tagaagaagg taaaacacca acccaagctg cgttacaagg ctcaaaagaa
      841 attgcttttg cggtcttggc gatgactttt accttagcag cagtctttgc cccgattgcc
      901 tttaccgaag ggcgaacagg taagctattt gccgaatttg ctttaacctt ggcggctgcg
      961 gtgattgttt cgggtttttg tgccttaacc ttatcgccga tgatgagttc aaaattattg
     1021 aaagctcatt caggccaaga aaatattatt tatcgagtga ttgaaacctt tttattagcc
     1081 ttgacgcgtg gttatcaaca attattaacc atagttttaa aattacgttg gttaatggtg
     1141 ctgattgctg tgggtgccag tgcttatatg tggctggtgt ttaaaggctt accgtctgaa
     1201 ttatccccca ccgaagatag aggagtgatt gtgtcgatgg gcattgcacc cgaaggtgtc
     1261 accactaatt atttagataa atacgcgcaa caactggaaa cgatttatag ccaagtgcct
     1321 gaacgcgctt cgaccttttt aattattggc atgcctgacg aaacacggtt tatgagtttt
     1381 aaccgtttta ttccgtggga acaacgagag cgtagtacac aagacattgc acccgattta
     1441 cggccaaaat tagcgggtat tgcaggggta aatgctttag ccacgttgcc accgtcatta
     1501 ggtcaaggta cgtctagtca accgattgat tttgtcttac aaaccacagg cagttatgcc
     1561 gatttaggca aaatgattgg tcaattaatg gaaaaaatca atcaaaatga gggttttgcc
     1621 aatccaattc ctgatttaaa actcaataag ccgcaattat cggtacaagt agacagagat
     1681 aaagccgctt tattaggtgt gggggtgtcg gatattggcc gaactttaga aactttatta
     1741 ggcagccgta aagtaaccac ctttaaatta gatggcgatc aatatgatgt gattttgcaa
     1801 cttgccgatg aagaacgccg tacccctgag caactcgatc aaatttatgt gcgtaatcaa
     1861 caaggtgaag tattaccgtt ggccagtgtg ttga
//
LOCUS       DAOQMB010000458         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_43254_length_1886_cov_12.572365, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000458 DAOQMB010000000
VERSION     DAOQMB010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43254_length_1886_cov_12.572365"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /gene="ettA"
                     /locus_tag="PLY05_11020"
     CDS             complement(<1..1088)
                     /gene="ettA"
                     /locus_tag="PLY05_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HNC03870.1"
                     /translation="MAQYIYTMNRVSKIVPPKREILKDISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGVDKDYQGEARAQAGIKVGYLAQEPQLDPSKDVRGNVEDGVREAVDALR
                     RLDEVFAEYAMEDADFDKLAAEQEKLEGIIQAWDAHNLDNQLEQAADALRLPAWDADV
                     TKLSGGERRRVALCRLLLSKPDMLLLDEPTNHLDAESVSWLERFLKEFTGTIVAITHD
                     RYFLDNVAEWILELDRGHGIPYQGNYSSWLEQKEARLESEQRQEEAHYKALKRESEWV
                     QQNPKARQAKSKARLSAYEELASKEFQKRNETQELYIPPGARLGDQVVEVRNISKVFG
                     DRLLYENLSFTVPKGAIVGIVGPNGAGKT"
     gene            1265..1792
                     /locus_tag="PLY05_11025"
     CDS             1265..1792
                     /locus_tag="PLY05_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNC03871.1"
                     /translation="MNKFILFTILLFLNTHSQADDLGDFIAHLAPKDPMPVALKPIAK
                     LQEAAISAISFVGKPYSFGGSNPDTGFDCSGLVQYVLDQSGVKKLPRTSREMYDYSSP
                     VDTDSLVVGDLLFFQTNNANQINHVGIYVGEGRFIHSPSTGNVIRLDKLAQNYWQKAF
                     VGGRRVLDLGQDTTK"
BASE COUNT          546 a          384 c          375 g          581 t
ORIGIN      
        1 gttttacccg caccgttcgg gccaacgata cccacaatcg cgcctttagg cacggtaaag
       61 ctgaggtttt cgtataataa acggtcgcca aaaaccttgc taatattacg cacttctacc
      121 acttggtcac ctaaacgtgc accaggtgga atgtacaatt cttgggtttc gttacgtttt
      181 tggaattctt tgctggctaa ttcttcataa gcactcaaac gcgctttgct cttagcttga
      241 cgtgctttag gattttgttg tacccattca gattcacgtt ttaaggcttt gtagtgcgct
      301 tcttcttggc gttgttcact ttctaagcga gcttcttttt gctctaacca agaagaataa
      361 ttaccttgat aaggaatacc atgaccacgg tcaagttcta aaatccattc ggccacattg
      421 tctaagaagt aacggtcgtg ggtaatcgcc acaatcgtgc ctgtaaactc ttttaagaaa
      481 cgttctaacc aagatacgga ttcggcatcc aaatggttgg ttggttcgtc taataacaac
      541 atatcaggtt tagacaacaa caaacgacac aaagccacac gacgacgctc accacctgat
      601 aatttggtaa catcggcatc ccatgcaggt aaacgtaagg catcagccgc ttgttcgagt
      661 tgattatcta agttgtgagc atcccatgcc tgaataatac cttctaactt ttcttgttcg
      721 gctgccaact tatcaaaatc ggcatcttcc atcgcatatt cagcaaaaac ttcatctaaa
      781 cggcgtaagg catcaaccgc ttcacgaaca ccatcttcca cattaccacg cacatctttg
      841 ctggggtcga gttgtggctc ttgtgccaaa taaccgactt taatgcctgc ttgagcgcgt
      901 gcttcgccct gatagtcttt gtcaacgcct gccataatgc gtaataaagt tgatttaccc
      961 gcaccattta aaccgagtac accaatcttt gcaccaggga agaaggataa agaaatatcc
     1021 tttaaaattt cacgcttcgg aggaacaatc ttcgaaacgc gattcatggt ataaatatat
     1081 tgcgccataa tgattaatca taagagtcca agatggcggc gattatagcg catttgtttt
     1141 gtggggttat cttttagtag gcttgattga aaaattatat ttgttgcgtc agtcacagat
     1201 aaaaacaatg ttttaaaata gcgtagggct ataatcagag tatatccttc aattgtttaa
     1261 aaatatgaat aaattcattt tatttaccat tttgttgttc ttaaatactc atagccaagc
     1321 tgatgactta ggtgatttta ttgcccattt agcacccaaa gacccaatgc ctgttgcttt
     1381 aaaaccgatt gctaaattac aagaagcggc tatatcagcg attagttttg tgggtaaacc
     1441 ttattcgttt ggtggtagta atcctgatac tggctttgat tgcagcggtt tggtgcaata
     1501 tgtgttagat caatcagggg tgaaaaaatt gccacgtacg tctcgtgaga tgtatgacta
     1561 tagcagtcca gtggatactg acagtttagt agtgggcgat ttattgtttt ttcaaactaa
     1621 taatgccaat caaattaatc atgttggcat ttatgtgggg gaagggcgat ttattcattc
     1681 gccctccaca ggtaatgtga ttcgtttaga taaattggca caaaactatt ggcaaaaagc
     1741 ctttgtgggt ggcagaagag tattagattt ggggcaggac acaacaaaat aaattgccga
     1801 cttgccgttc aaagattgct cattttaaag atgagagaaa atcattatcg tttatcaata
     1861 agcatgttaa aatataaaat ctaatt
//
LOCUS       DAOQMB010000459         1885 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_43301_length_1885_cov_7.070492, whole genome shotgun sequence.
ACCESSION   DAOQMB010000459 DAOQMB010000000
VERSION     DAOQMB010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43301_length_1885_cov_7.070492"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..1218)
                     /gene="sucC"
                     /locus_tag="PLY05_11030"
     CDS             complement(61..1218)
                     /gene="sucC"
                     /locus_tag="PLY05_11030"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HNC03872.1"
                     /translation="MNLHEYQGKQLFAEYGLPVSKGFAASTPEEARQAAEQIGGDIWV
                     VKAQVHAGGRGKAGGVKLVKTLDEVEQFAREKLGTNLVTYQTDSKGQPVSKILVETCT
                     DIAKELYLGAVVDRASRSIVFMASTEGGVEIEKVAHETPEKIFKVTIDPLIGAQAYQG
                     REVAGKLGLNATQTKQFVKIYLGLAKMFVECDLAMIEVNPLVVTKEGNLHCLDAKIVV
                     DSNAVYRHPKLKAMDDPSQSDERERRAAEWELNYVALEGNIGCMVNGAGLAMATMDII
                     KLKGGQPANFLDVGGGATKQRVTEAFKIILSDSAVKAVLVNIFGGIVRCDMIAEGIIG
                     AVQEVGVKVPVVVRLEGNNAELGAQKLRDSGLNIIAATSLNEAGELVVKQV"
     gene            complement(1371..>1885)
                     /locus_tag="PLY05_11035"
     CDS             complement(1371..>1885)
                     /locus_tag="PLY05_11035"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996415.1"
                     /note="E3 component of 2-oxoglutarate dehydrogenase
                     complex; catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNC03873.1"
                     /translation="TNVEGVYAIGDLVRGPMLAHKAMEEGMMVAELIAGHYAQINYDT
                     IIGVIYTHPEIAWVGKTEEQAVAAGIEVKTGQFPFQVNGRALAANDAQGLVRFVADAK
                     TDRLLGMAVIGSNAGDMVHQGMIALEFAASIEDLQLMTFAHPTLSEAVHEAALSVDGR
                     AIHAIQRKRK"
BASE COUNT          524 a          468 c          329 g          564 t
ORIGIN      
        1 ataccttggc aaataacttt ggtattttta tcaattaaaa cgctcattat ctgcccctaa
       61 ttacacttgt ttgacaacta attcgcctgc ttcatttaac gaagtagccg caataatatt
      121 taaaccactg tcacgcaatt tttgtgcgcc caattcggca ttgtttcctt ctaaacgcac
      181 cacaacaggc actttgacac ctacttcttg tactgcgcca ataatacctt cagcgatcat
      241 gtcacaacgg acaataccac caaagatgtt aactaatacg gcttttacag cagagtcaga
      301 taagatgatt ttgaaggctt cggtcacacg ttgtttagtt gcaccgccac ctacgtctaa
      361 gaagttggca ggttgtccac ctttaagttt gatgatgtcc atcgtggcca tcgccaaacc
      421 tgcaccattc accatacaac caatgttacc ttctaaagcc acatagttga gttcccattc
      481 ggccgcacgg cgttcacgct catcagattg gcttgggtca tccatcgctt ttaattttgg
      541 atgacgatat acagcgttag aatccaccac aattttggca tctaaacaat gtaagttacc
      601 ttctttagtc actactaaag gatttacttc aatcatggct aagtcacact cgacaaacat
      661 tttcgccaaa cctaaataga ttttgacaaa ttgtttggtt tgagtggcat ttaaacctaa
      721 cttacctgca acttcacgac cttgataagc ttgtgcacca atcaatggat caatggttac
      781 tttgaagatt ttctctggtg tttcatgagc aactttctca atttcaacac caccttctgt
      841 tgaagccata aaaacaatgc tacggcttgc acgatcaaca acagcaccta aatacaactc
      901 tttagcgata tcagtacaag tttcaaccaa aatcttgcta acaggttgac ctttgctatc
      961 ggtttgatag gtcactaaat ttgtacctaa tttctcgcgt gcaaattgtt caacttcgtc
     1021 taaagtttta accagtttta ccccacctgc tttaccacga ccacctgcat gaacttgagc
     1081 tttaaccacc caaatatcac caccaatttg ttcagccgct tgacgagctt cttctggtgt
     1141 cgatgcggca aagccttttg acacaggcaa tccatattca gcaaataatt gcttgccttg
     1201 atattcatgt aaattcattg tttcaccctg ttcatattta aagcctatgg ctttgtgacg
     1261 cttattatcc gcttttgacg gaatactaca ttatgttttc ctcactttag ctctctccct
     1321 gaaagagaaa actccctcgc cctttgggag agggttgggg tgagggcgaa ttatttacgt
     1381 ttacgctgaa tggcgtgaat ggctctgcca tctaccgaca aagcagcctc atgtacagct
     1441 tcagacaatg taggatgggc aaaggtcatt aattgcaaat cctcaatgct tgccgcaaac
     1501 tctaaagcaa tcatgccttg atgcaccata tcccctgcat ttgaaccaat gacagccatg
     1561 cctaacaaac gatcagtttt agcatcagcg acaaaacgta ctaaaccttg tgcatcatta
     1621 gccgctaaag cacgaccatt cacttggaat gggaattgac ctgttttgac ttcaatgcct
     1681 gcagcaacgg cttgttcttc ggttttgcca acccatgcaa tttcgggatg ggtataaatc
     1741 acgccaataa tagtgtcgta gttgatttgg gcgtaatgac ctgcaatcaa ttcggcaacc
     1801 atcatgcctt cttccatagc tttatgggct aacattgggc cacggactaa gtcaccaatc
     1861 gcataaacgc cttctacatt agtac
//
LOCUS       DAOQMB010000460         1883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_43385_length_1883_cov_16.578775, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000460 DAOQMB010000000
VERSION     DAOQMB010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43385_length_1883_cov_16.578775"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..>1883
                     /locus_tag="PLY05_11040"
     CDS             6..>1883
                     /locus_tag="PLY05_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC domain/YcaO domain-containing protein"
                     /protein_id="HNC03874.1"
                     /translation="MEIKVNFLDKLRLEAKFDDFTVIADQPIRYKGDGSAPGPFDYFL
                     ASSALCAAYFVKLYCQTRNISTDNIRLSQNNIVDPENRYKQIFKIQVELPADISDKDR
                     QGILRSIDRCTVKKVVQTMPDFVIEDVESIDADAQALLMSNLTSENNTYILGKDLPLE
                     QTIANMSSIMANLGMKIEIASWRNIVPNVWSLHIRDAHSPMCFTNGKGASKESALASA
                     LGEFIERLNCNFFYHGQFWGEDIANADFVHHPDEKWFKPALRNRLPREILDDYCREIY
                     NPDGELRGSHLYDTNSGNVKRGICCLPFVRQSDGQVVYFPSNLIENLFLSNGMSAGNT
                     IAEAQVQCLSEIFERAVKREILEGEMALPDVPQDVLAKYPHIVAGIKGLEEQGFPVLV
                     KDASMGGKYPVMCVTLMNPRTGGVFASFGAHPSFEVALERSLTELLQGRSFEGLNDLP
                     RPTFQSNAVTEPNNFVEHFIDSSGLVSWRFFSAKANFEFVEWDFSSQGENANVEEAAT
                     LFGILKDLGKEVYMAVYTELGAPACRILVPNYSEIYPVEDLIWDNTNKALLFREDILN
                     LHRLDDNKLAKLLERLEESDLDDYTDIVTLIGIEFDDNTPWGQLTILELKLLIHLALQ
                     QF"
BASE COUNT          515 a          366 c          421 g          581 t
ORIGIN      
        1 ctctcatgga aattaaagtc aattttcttg acaaacttcg actcgaagca aagtttgatg
       61 acttcacggt aatcgccgac caaccgattc gatacaaagg tgatggttct gcacccggcc
      121 catttgatta ctttttagcg tcttctgcct tatgtgccgc gtattttgtt aagttgtatt
      181 gccaaacacg caacatttcc actgataaca ttcgtctgtc gcaaaacaac attgttgacc
      241 ccgaaaatcg ctataaacaa atttttaaga ttcaagtcga actgccagcc gatatttcag
      301 acaaagaccg acaaggcatt ttgcgctcaa tcgaccgttg taccgttaaa aaagtggtac
      361 aaaccatgcc cgattttgtt attgaagacg ttgaaagtat tgatgccgat gcgcaagcct
      421 tgttaatgtc caatctaaca tctgaaaata atacttatat tttgggcaag gatttacccc
      481 tagaacaaac cattgccaat atgtcgagca ttatggctaa tttgggcatg aaaatcgaaa
      541 tcgcctcgtg gcgtaatatt gtgccaaatg tctggtcgtt gcatattcgg gatgcccatt
      601 cgcccatgtg ttttaccaac ggtaaaggcg caagcaaaga aagtgcctta gcctccgcat
      661 taggtgagtt tattgagcgg ctcaactgca actttttcta tcatggtcag ttttggggtg
      721 aggacatcgc taatgccgat tttgtccatc accccgatga aaaatggttt aagccagccc
      781 ttagaaatcg tttacccaga gaaattttag acgactattg ccgcgaaatt tataaccctg
      841 atggcgaatt acgcggttca catttgtatg acactaattc gggtaatgtc aaacgtggca
      901 tttgttgttt gccttttgtc cgtcaatcgg atggccaagt ggtgtatttt ccgtctaatt
      961 tgattgaaaa cttattttta agtaatggca tgagcgcagg caacaccatt gccgaagcac
     1021 aagtacagtg tttatccgaa atttttgagc gtgcggtaaa acgcgaaatt ttagaagggg
     1081 agatggcctt acccgatgtg ccacaagacg tattggccaa atatccgcat attgtggcag
     1141 gcatcaaagg cttagaagag cagggctttc ccgtgttagt caaagatgcg tcaatgggtg
     1201 gtaaatatcc tgtgatgtgt gtgaccttga tgaacccacg gacaggcggt gtgtttgcct
     1261 catttggcgc acatcccagt tttgaagtcg ccttagaacg tagtttaacc gagttattac
     1321 aagggcgtag ttttgaaggt ttaaatgact tgcctcgacc tacctttcaa agcaatgcgg
     1381 tcactgaacc caataatttt gtcgaacatt ttattgattc gagtggtttg gtgtcgtggc
     1441 ggttttttag tgccaaggcc aattttgagt ttgtcgagtg ggattttagt agtcaaggtg
     1501 agaacgctaa tgttgaggaa gcggctacct tgtttggtat tctcaaagac ttgggtaaag
     1561 aagtgtatat ggccgtttac accgaattgg gcgcgcctgc ttgccgtatt ttagtgccta
     1621 attattccga aatttatcct gttgaagatt tgatttggga taataccaat aaggctttgt
     1681 tgttccgtga agatatttta aatttacatc gtttggatga taacaaatta gctaaattac
     1741 ttgagcgttt agaagaaagt gatttggatg attacaccga tattgtcacc ttaattggta
     1801 ttgagtttga cgacaatacc ccgtggggac aattaaccat tttagaatta aagctattga
     1861 ttcatttagc tttacagcag ttt
//
LOCUS       DAOQMB010000461         1868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_44032_length_1868_cov_16.243243, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000461 DAOQMB010000000
VERSION     DAOQMB010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44032_length_1868_cov_16.243243"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..551
                     /locus_tag="PLY05_11045"
     CDS             90..551
                     /locus_tag="PLY05_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006581852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC03875.1"
                     /translation="MNNWQPHVTVAVIVERDGKFLLVEEEILDSHVDVYNQPAGHVEH
                     NETLIEAAKREALEETGFEVEPTALLGIYTYTPPNKPDTTYYRVCFIAKAIHHYERPL
                     DEGIIRAVWLTLDELVTSNRARSPLVIKCIQDYLRGQSFPLSAIYEHPTSQ"
     gene            548..1672
                     /gene="mnmA"
                     /locus_tag="PLY05_11050"
     CDS             548..1672
                     /gene="mnmA"
                     /locus_tag="PLY05_11050"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011960231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNC03876.1"
                     /translation="MNANLTSSTATTRPKVIVGMSGGVDSSVSAYLLLQQGYDVEGLF
                     MKNWEEDDGTDYCTAKEDLKDAQAVCDKLGIKLHTANFAAEYWDRVFEHFLAEYQAGR
                     TPNPDILCNKEIKFKAFLDYALVLGADFIATGHYTRRSADSDCAQLLKGLDPNKDQSY
                     FLHAVNGKQIAKTLFPVGDIEKPEVRRIAAEQGFINHAKKDSTGICFIGERRFRDFLQ
                     QYLPAQKGEIVTDEGKVIGEHLGLMYYTLGQRQGIGVGGVKNSAEAPWFVVAKDLNTN
                     RLIIGQGHEHPLMMSRSLYSEKLDWVAGHLPELPLRCKAKTRYRQADQDCVIHAEGNG
                     VRVVFDQAQRAVTAGQSVVFYLEDVCLGGGVIESTELWTA"
     gene            1669..>1868
                     /locus_tag="PLY05_11055"
     CDS             1669..>1868
                     /locus_tag="PLY05_11055"
                     /inference="COORDINATES: protein motif:HMM:NF016259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF489 family protein"
                     /protein_id="HNC03877.1"
                     /translation="MSNIHRQKVLALAAVFQAAALADHLARRGTADTTAIKTLLDSII
                     VFDTDNPEAIYGTPQQLLIGLKS"
BASE COUNT          543 a          370 c          399 g          556 t
ORIGIN      
        1 cctttctcat ctaaatgtgt taaagacatg gattctctct ataatattaa attgagcctg
       61 tttggctaat atatcaagga gtataacaga tgaataactg gcaaccccat gtcactgttg
      121 cagtgattgt tgagcgcgac ggcaaatttt tgttagtcga agaagaaatt ttagattctc
      181 atgtcgatgt ctataatcaa cctgctggtc atgttgagca taacgaaacc ttaattgaag
      241 cagcaaaacg cgaagcactc gaagaaacag gctttgaagt tgagccaact gctttactgg
      301 gtatatatac ctatacccca ccaaacaagc cagataccac ctattatcgt gtgtgtttta
      361 ttgctaaagc gatacatcat tatgaaagac cactggatga aggcattatt cgtgcggtgt
      421 ggttaacctt agatgaatta gtaacgtcta atcgcgcgcg cagtccttta gtgataaaat
      481 gtattcaaga ctatttacgt ggtcaatctt ttcctttatc tgcgatttat gagcatccta
      541 cgtcacaatg aacgctaatt taacttcttc caccgccacc actcgtccaa aagttattgt
      601 tggtatgtct ggcggtgttg attcttctgt atccgcatat cttcttcttc aacaaggtta
      661 tgatgttgaa ggcctgttta tgaaaaactg ggaagaagat gacggcactg actattgcac
      721 agccaaagaa gatctcaaag atgctcaagc tgtgtgtgac aaattaggca tcaaactaca
      781 taccgctaat tttgctgcgg aatattggga cagagtattt gagcatttct tggcagaata
      841 ccaagcaggt cgtaccccaa accctgatat tttatgtaat aaagaaatca agtttaaggc
      901 atttttagat tatgcccttg tgttaggtgc tgattttatt gccactggtc attatacccg
      961 tcgtagtgcc gatagtgatt gtgcacaatt actcaaaggt ctagacccca acaaagatca
     1021 aagttatttt ttacatgctg ttaatggtaa acagatcgcc aaaaccttat tccctgtggg
     1081 tgatattgaa aagcctgaag tgcgtcgtat tgccgccgaa caaggtttta ttaatcatgc
     1141 caaaaaagac tcgacaggca tttgttttat tggtgaacgt cgttttcggg acttcttaca
     1201 acaatactta cctgcacaaa aaggtgaaat tgtcaccgat gaaggcaaag tcattggtga
     1261 acatttaggc ttaatgtatt acaccttggg acaacgtcaa ggtattggtg tgggtggtgt
     1321 taaaaacagt gctgaagccc cgtggtttgt tgtcgcaaaa gatttaaaca ccaatcgttt
     1381 gattattggt caaggccatg aacacccctt aatgatgtca cgtagtttat atagcgaaaa
     1441 acttgattgg gttgcaggcc acctacccga attaccctta cgttgcaaag caaaaactcg
     1501 ctatcgccaa gccgatcaag attgtgtgat tcatgctgaa ggcaatggtg tacgtgtagt
     1561 gtttgatcaa gcacaacgtg ccgtcacggc tgggcaatcc gtggtgtttt atcttgagga
     1621 tgtgtgttta ggtggtggag tgatcgaaag cacggagtta tggacagcat gagcaatatt
     1681 catcggcaaa aagttttagc cttagcagca gtctttcagg ctgctgcttt agccgatcat
     1741 ttagcacgtc gagggacggc tgataccact gcaatcaaaa cactacttga tagcattatt
     1801 gtctttgata ccgacaatcc tgaagccatt tatggtacac ctcaacaatt attaatcggc
     1861 ttaaaatc
//
LOCUS       DAOQMB010000462         1861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_44332_length_1861_cov_8.320598, whole genome shotgun sequence.
ACCESSION   DAOQMB010000462 DAOQMB010000000
VERSION     DAOQMB010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44332_length_1861_cov_8.320598"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..1534
                     /locus_tag="PLY05_11060"
     CDS             206..1534
                     /locus_tag="PLY05_11060"
                     /inference="COORDINATES: protein motif:HMM:NF023726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3631 domain-containing protein"
                     /protein_id="HNC03878.1"
                     /translation="MAAKRKESQQTQDKQEAGTSTIFEEVSEWPHSVDGALLLQDITK
                     IVQRFTVLNIEQARACALWIAFTWFIDGAKVAPILNITSPEKRCGKSTLLLVIESMVL
                     KALLASNITGAALFRAVELWTPTLLIDETDAFLNEQEEMRGIINAGHYRRTAFVIRTT
                     GDNHEPKKFTTWCAKVLCGIGKIANTLIDRSIVIELRRKLTSEQVENIHLANEDEFSV
                     ICQKLKRWSQDNFEQYREKRPKRIEGINDRAADNWQPLQSVAELAGKEWPNLCRLAAI
                     QLSGIEEETPSINVELLMDIADIFHDRGMTKIFTADLIEALCQNEEKAWATWNRGKPI
                     TAHQVSKKLSEFKIKPDDVRIGTIHKKGYKLQDINEALIRYKPTPDENATPRQASNTN
                     AYSDFEKRDNDKMSRTQKPLEPLINKDCRGVADKSDMAHPQTIIDDVEVF"
     gene            1534..>1861
                     /locus_tag="PLY05_11065"
     CDS             1534..>1861
                     /locus_tag="PLY05_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03879.1"
                     /translation="MTATDLYNDLVLHGFSLSVQGGNLILSPSSQLSQAQRLEIKQHK
                     SELLLLLDTNNPHTKGEIKPVLKVVAKSELPKDRADLIHQLETQVKSESHSQTVDGRL
                     NLLVNPQ"
BASE COUNT          619 a          368 c          414 g          460 t
ORIGIN      
        1 ccattatcgg gtaagggcaa cttatgccta aaccgtaatt tgtgcaacaa agccaaggga
       61 aagtatagac gatgttggca atggtggcga gagtgaagat gcagccattg aaagattatc
      121 tcttttatcg gctattgaat atgagcgtgt cagagttgat gaagccaaaa agctaaatat
      181 ccgtgccagt gttttagata aattggtggc ggccaaacgt aaagaaagcc aacaaacaca
      241 ggacaagcaa gaagcaggaa caagcaccat ttttgaagaa gttagcgaat ggcctcactc
      301 ggttgatggt gcattgctct tgcaagatat aaccaagata gtacagcgat ttaccgtatt
      361 aaacattgag caagcgcgag cctgtgcgtt atggatagcc tttacttggt ttattgatgg
      421 tgcaaaagtt gcccctattc ttaacattac cagtcctgaa aaaaggtgtg gtaagtccac
      481 tttgttgctt gtcattgaat caatggtgtt aaaggcttta ctggcctcaa atatcacagg
      541 cgcggcttta tttcgtgccg ttgagttatg gacaccgaca ctgttaattg acgagacaga
      601 cgcatttttg aatgagcaag aagaaatgcg cggcataatc aatgcaggac actacagacg
      661 caccgccttt gtgattcgta ccactggcga caatcacgaa cctaaaaagt ttacgacttg
      721 gtgtgcaaag gtactttgtg gtatcggtaa aattgctaat accttaatcg accgaagtat
      781 tgtcattgaa ctaagacgaa aattaaccag tgaacaagtc gaaaatatcc acctagccaa
      841 tgaggacgaa tttagcgtta tttgccaaaa gttaaagcgt tggtcacaag ataactttga
      901 gcagtatcga gagaaacgac ctaaacgcat agagggcatt aatgaccgtg cagccgataa
      961 ctggcaaccc ttacaatctg ttgcagaatt agcaggcaaa gaatggccta atttatgccg
     1021 tttagccgct attcagttat caggtattga agaagaaacg ccaagtatca atgttgagct
     1081 attgatggat attgccgata tattccacga tagaggcatg accaagatat ttaccgccga
     1141 cttaatcgaa gcactttgtc aaaatgaaga aaaggcatgg gcaacatgga atagaggtaa
     1201 accaataacc gcgcatcaag tcagtaaaaa actgtcagag tttaaaatta aacctgatga
     1261 tgttcgtatt gggacaatcc ataaaaaggg ctacaagctt caagatataa atgaggcatt
     1321 aattcgttac aagcctaccc ctgatgaaaa cgcgacaccg cgacaagcta gtaataccaa
     1381 tgcttacagc gattttgaga aacgcgacaa tgacaaaatg tcgcggacac aaaaaccgct
     1441 agaacccttg ataaataagg attgtcgcgg tgtcgcggat aaaagcgaca tggcacaccc
     1501 acaaacaatc attgatgacg tggaggtgtt ttaatgaccg ccaccgattt atataacgac
     1561 cttgtgttac atggttttag tttgtcggta caaggtggca atttgatatt gtcacccagt
     1621 agtcaactta gccaagcgca acgattagag attaaacagc ataaaagcga gttacttctt
     1681 ttactggata ctaacaaccc tcacaccaaa ggagagataa aacctgtttt aaaggtagtt
     1741 gccaagtcag agctacccaa agatagagca gacttgattc accaactgga gacgcaagtt
     1801 aaaagcgaat cccattcaca gactgtggac ggacgactta atttgttggt caatcctcaa
     1861 a
//
LOCUS       DAOQMB010000463         1860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_44367_length_1860_cov_18.448199, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000463 DAOQMB010000000
VERSION     DAOQMB010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44367_length_1860_cov_18.448199"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PLY05_11070"
     CDS             complement(<1..446)
                     /locus_tag="PLY05_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HNC03880.1"
                     /translation="MSYIIDRRLNGRHKSMVNRQRFLDRYKAQIRESVADAASKRSIT
                     DMERGESVSLPTKDISEPVFHHGQGGVRDMVHTGNKEFLSGDKVQRPQSGGGGQGSGK
                     ASNQGQGEDEFSFTLSQEEFLNFLFEDLALPNLIKRRLAGIETWEW"
     gene            complement(550..>1860)
                     /locus_tag="PLY05_11075"
     CDS             complement(550..>1860)
                     /locus_tag="PLY05_11075"
                     /inference="COORDINATES: protein motif:HMM:NF011999.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HNC03881.1"
                     /translation="VSKTEPGDENNQDISALVGKVDIRKLEDFPQSDADAYSFSGGLC
                     KANRGLMEFVEMFKAPIKVLHPLLTATQEGNYNGTEAIGPIPFDGVILAHSNESEWQN
                     FRNNKNNEAFIDRIFIVKVPYCLRVSEEIQIYQKLIDSSSLKKAPCAPDTLRMLAQFT
                     VLSRLKDTENSSIYSKMRIYDGENLKDTDPRAKSHQEYKDAAGVDEGMNGLSTRFAFK
                     ILSKVFNYDHSEVAANPVHLLHVLETQIEQEQYANETQARYLRFIKEFLAPRYVEFIG
                     KEIQTAYLESYSEYGQNIFDRYVQYADFWIQDQEFRDPDTGEIFNRQALNEELEKIEK
                     PAGISNPKDFRNEVVNFVLRARANYNGKNPSWLSYEKLRAVIEKKMFSNTEDLLPVIS
                     FNPKANKNDQQKHQDFVRRMVERGYTEKQVRLLCEWYLRFRKAQ"
BASE COUNT          535 a          395 c          369 g          561 t
ORIGIN      
        1 tgccattccc atgtttcgat gcctgcaagt cgtcttttaa tcaggttggg tagggcaagg
       61 tcttcaaaca aaaagtttaa aaactcttct tgagagaggg taaaactaaa ttcatcttcg
      121 ccttgccctt gattgctggc tttgcccgag ccttgaccac cgccaccact ttgagggcgt
      181 tgtactttat caccgctcaa aaactctttg ttaccagtat gcaccatatc acggacacca
      241 ccttgaccat gatgaaatac aggctcagaa atatctttgg tgggtaagga caccgattcg
      301 ccacgctcca tatcggtaat gctacgttta ctggcagcat cagcaacaga ttcacgaatt
      361 tgggctttat aacgatctaa aaagcgttgg cgattaacca tgcttttatg gcggccattt
      421 aaacgacggt cgatgatata gctcatgtgt tctccagcat taactgctcc tccccctaat
      481 ttagggggag gctgggaggg ggtagaggta gaaccccacc ctaaccctcc cctaaattag
      541 gggaggggat tattgcgctt ttctaaagcg taagtaccat tcgcataata aacgtacttg
      601 tttttcagta tatccacgtt caaccatacg acggacaaaa tcttgatgtt tttgttggtc
      661 gtttttgttg gctttggggt taaaggaaat aacaggcaac aagtcttcgg tgttggagaa
      721 catttttttc tcaatcacgg cacgcagttt ttcgtaagat aaccatgatg gatttttgcc
      781 attatagtta gcacgagcac gtaacacgaa attgaccact tcattacgga agtctttggg
      841 attagaaatg cccgcaggct tctcgatttt ttctagctcc tcatttaacg cttgacggtt
      901 aaagatttcg cccgtatcag gatcacggaa ctcttgatct tgaatccaga agtcagcata
      961 ttggacataa cgatcaaaga tattttggcc atattcagaa taagactcca aataagcagt
     1021 ttgaatttct ttgccaataa actcgacata acgtggtgct aaaaactctt taataaagcg
     1081 taagtaacgt gcttgggttt cattggcata ttgctcttgc tcaatttgag tttctaaaac
     1141 atgcaataaa tgaacagggt tagcagcgac ctctgaatga tcataattaa agactttgga
     1201 taaaatcttg aaagcaaaac gagtggataa accattcatg ccttcatcaa cacccgcagc
     1261 atctttatac tcttgatgtg attttgctct tggatcggtg tcttttaaat tctcaccatc
     1321 ataaatccgc attttggaat aaatgctaga gttttcggtg tcttttaaac gcgacaacac
     1381 ggtaaattgc gccaacatgc gtaaagtatc aggcgcacaa ggagcttttt tcagtgaaga
     1441 actatcaatc aatttttgat aaatttggat ttcttcacta acacgtaaac aataaggtac
     1501 tttgacaata aaaatacggt caataaatgc ctcattattt ttattgtttc taaagttttg
     1561 ccattctgac tcgttggagt gtgccaaaat cacgccatca aagggaatag ggccaatcgc
     1621 ctctgtccca ttgtaattac cttcttgggt ggcggtaagc aaaggatgta gcactttaat
     1681 cggtgcttta aacatctcaa caaattccat taagccacga ttggctttac acaaaccacc
     1741 tgagaatgaa taggcatcgg catcgctttg tggaaagtct tccagtttac gaatatcaac
     1801 tttacccaca agtgcggaaa tgtcttgatt attctcatca ccaggttcag ttttagaaac
//
LOCUS       DAOQMB010000464         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45127_length_1844_cov_14.682504, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000464 DAOQMB010000000
VERSION     DAOQMB010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45127_length_1844_cov_14.682504"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            215..721
                     /locus_tag="PLY05_11080"
     CDS             215..721
                     /locus_tag="PLY05_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC03882.1"
                     /translation="MSNASKQLCQIIAEDIMTTPVLTAHDTWSVKMLLDFFAQHHITG
                     VPVTNYQGELKGVVTMSDILRFENLPAIEKERLIGVSCYGEYSNYQLSHHDLEQLVLH
                     ADINCPVSQIMTPHIITVDKSVSILAIAKLFLEKSIHRVFVEQNQQLVGVISTSNLLA
                     AMIQSCDC"
     gene            complement(740..1300)
                     /locus_tag="PLY05_11085"
     CDS             complement(740..1300)
                     /locus_tag="PLY05_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNC03883.1"
                     /translation="MLRKNPHGDWPQVHESAFIDPTAILCGQVIIGENVFIGPYAVIR
                     ADEVNEYGEMEPIVIGAHSNIQDGVVIHSKAGGLVVIGEYTSIAHRSIIHGPCTIGSH
                     VFIGFNSVVYNATIHDEVVVRHNCVIDSREILAGLYVPSCQKVNHETDTRLLKPASEY
                     AHDFSESVAKANHSLVQGYRAIRNEF"
     gene            complement(1302..>1844)
                     /locus_tag="PLY05_11090"
     CDS             complement(1302..>1844)
                     /locus_tag="PLY05_11090"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNC03884.1"
                     /translation="KKRLDAPTVLRELIALEKNPYQDHKNWSEVSNADLIHHICSHYH
                     DKHRQQLPELIRLAAKVERVHGEKADCPHGLAQLLSETLDELEQHMLKEEEVLFPMIV
                     HNRLAQAQMPIHVMEHEHIDHGQRLEKLLLLTNQGVPPQGACNTWQALYLGVRSFAQD
                     IIEHIHLENNVLFARCLQGA"
BASE COUNT          572 a          392 c          319 g          561 t
ORIGIN      
        1 agtgttacgc tattttttaa gctttttacg acaagtcttg ataaaaaaag acaaattaat
       61 cgctcagact atacttttgg ctaatgtctc ctagccatct gactaatatc acttatttaa
      121 aagacttcta tactaaaaat tccctactct tctctaggac ttacacacta atttgagaaa
      181 atggtgtcag tcctatttta aaaggaccgt catcatgagc aatgcctcca aacaattgtg
      241 ccaaattatc gcagaagaca ttatgacaac tccagtttta acagctcatg acacttggtc
      301 ggtaaaaatg ctgttagatt tttttgcaca acatcatatt actggtgtac cagtcaccaa
      361 ttatcaaggg gaactaaaag gtgtcgtgac aatgagtgac atcttacgct ttgaaaattt
      421 gccagcgata gaaaaagaac gtctgattgg cgtgtcttgt tatggggaat attcaaacta
      481 tcaactcagt catcacgatt tagaacaatt agtcttacat gctgatatca attgccctgt
      541 cagccaaatt atgacgccac atatcattac tgtggataaa tccgtttcta tcttagcaat
      601 agcaaaattg tttttagaga aaagtattca tagggtgttt gtagagcaaa atcagcaact
      661 cgttggtgtg attagcacaa gcaatctatt agcagctatg attcaaagct gtgattgtta
      721 ggggcttatc agcccctttt taaaactcat tacgaatcgc tctatatcct tgaacaagac
      781 tatgattggc tttggcgaca ctttccgaaa agtcatgggc atattctgag gcaggcttta
      841 gcaaacgtgt atcggtttcg tggtttacct tttggcagga aggcacgtac aaccctgcta
      901 aaatttctcg gctatcaatc acacagttat ggcgtaccac cacttcatca tgaatagttg
      961 cgttataaac caccgaatta aaaccaataa acacatgact accaatagtg caagggccat
     1021 gaataattga acgatgggca attgacgtat attcaccaat aaccaccaaa ccacccgctt
     1081 tagagtgaat caccacgcca tcttgaatgt tagaatgtgc accaatgacg atcggttcca
     1141 tttcaccata ttcattcacc tcatcggctc taatcacggc ataaggccca ataaagacat
     1201 tttcgccaat aatcacttga ccacacaaaa tcgctgttgg atcaataaag gcactttcgt
     1261 gaacttgcgg ccaatcacca tgaggatttt tgcgtaacat gttaagcccc ttgtaagcaa
     1321 cgagcaaata acacattgtt ttctaaatga atatgctcaa taatatcttg tgcaaaacta
     1381 cgtacaccca aatataaagc ttgccatgta ttacaagctc cttgaggtgg cacaccttga
     1441 ttagtcaata acaataattt ttctaaacgc tggccatgat ctatatgttc atgttccata
     1501 acatgaatcg gcatttgggc ttgagctaaa cgattatgaa cgatcattgg aaacaacacc
     1561 tcctcttctt tgagcatatg ttgttctaac tcatctaatg tctcactcag taattgagcc
     1621 aaaccatgtg gacaatctgc tttttcacca tgcactcgct caactttggc cgctaaacga
     1681 attaactctg gtaactgctg acgatgttta tcatgataat gactacagat atggtggatt
     1741 aagtcggcat tactaacttc tgaccaattt ttatggtctt gataaggatt tttttctagc
     1801 gcaattaact ctcgtaacac agttggtgca tctaatcgtt tttt
//
LOCUS       DAOQMB010000465         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45175_length_1843_cov_15.303691, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000465 DAOQMB010000000
VERSION     DAOQMB010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45175_length_1843_cov_15.303691"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..903
                     /locus_tag="PLY05_11095"
     CDS             154..903
                     /locus_tag="PLY05_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNC03885.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADKTGVDLANVKMAINPFCEIAVE
                     EAVRLKEKGIVSEIVVVSIGPKEAQEQIRTAMALGADRGILVETGSEVQPLAIAKILK
                     AICDQEQPQLVIVGKQAIDDDNNQTGQMLAALAGYGQGTFASVVKVEGGKAIVTREID
                     GGLQTVELNLPAVVTTDLRLNEPRYAALPNIMKAKKKPLDTKTPADYGVDVTPRVKTL
                     KVEPPAERSAGIKVKTVEELVAKLKNEAKVI"
     gene            905..1834
                     /locus_tag="PLY05_11100"
     CDS             905..1834
                     /locus_tag="PLY05_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005263505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HNC03886.1"
                     /translation="MAVLVYAEHDNSSIKGATLNTVAAAQAIGGEIHVLVAGSGCQAA
                     AAAAAQIAGVSKVLVADNAAYANQLAENVSLLVTEIGKGYSHILAAATTNGKNFAPRV
                     AALLDVAQISEITKVVSADTFERPIYAGNAIATVQALDAIKVITVRSTGFDAVAATGG
                     SAAVEAVGSATDAATSKFVGEELAKSDRPELTAAKIVVSGGRGMGNGDNFKILYSLAD
                     KLGAAVGASRAAVDAGFVPNDMQVGQTGKIVAPQLYVAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGIVGDLFDLVPQLEKLV"
BASE COUNT          521 a          367 c          428 g          527 t
ORIGIN      
        1 ttgtcaatgg ccagaaaaac aggttaattc gtcactgttc gcttgaccaa aaagcagcaa
       61 tatccgaaga tattgcacct tttatattgt ctgcgttttt tgaccagata atacccttac
      121 cacttcgtta aatcgtcaca ttccgaggat tccatgaagg ctcttgttgc tgtaaaacgc
      181 gttgtagatt acaacgtaaa agttcgcgta aaagcggata aaacaggtgt tgaccttgcc
      241 aacgttaaaa tggctatcaa cccattctgc gaaatcgccg ttgaagaagc cgttcgttta
      301 aaagaaaaag gcattgttag cgaaatcgtt gttgtttcta ttggtccaaa agaagctcaa
      361 gaacaaattc gtactgcgat ggctcttggt gcagaccgcg gtattttggt agaaactggt
      421 tctgaagtac aacctttagc cattgccaaa attttaaaag ctatttgcga tcaagaacaa
      481 ccacaattag tgattgtggg taaacaagcg attgatgacg ataacaacca aactggccaa
      541 atgttggctg ctttagcagg ttatggtcaa ggtacgtttg cttctgttgt taaagtagaa
      601 ggcggcaaag caatcgttac tcgcgaaatc gacggtggtt tacaaactgt tgaattaaac
      661 ttgccagctg ttgtaacaac agacttacgt ttgaacgagc cacgttatgc agctctacca
      721 aacatcatga aagcgaagaa aaagccttta gataccaaaa cgccagccga ttatggtgtt
      781 gacgtaacgc cacgcgttaa gaccttaaaa gttgagccgc cagccgagcg cagtgcaggt
      841 atcaaagtga agactgttga agagttggtt gctaaactca agaacgaagc aaaggtgatc
      901 taatatggca gttttagttt acgcagaaca cgataatagt tctattaaag gtgctaccct
      961 taacactgtt gcggctgctc aagcaattgg tggcgaaatc cacgttttag ttgcaggttc
     1021 tggctgtcaa gctgctgccg ctgctgctgc acaaatcgct ggcgttagca aagtattggt
     1081 tgctgacaat gctgcttatg ctaatcaatt agctgaaaac gttagcttgt tagtgactga
     1141 aattggtaaa ggttatagcc acattttagc ggctgctact accaatggta aaaactttgc
     1201 accacgcgtt gctgcattgt tagacgttgc tcaaatttct gaaatcacta aagtagtgag
     1261 tgcagataca tttgaacgtc caatttacgc aggtaacgca atcgctactg ttcaagcttt
     1321 agacgccatc aaagttatca ctgtacgttc tacgggtttt gatgctgttg ctgcaacagg
     1381 tggttctgct gctgttgaag cagttggctc tgccactgat gctgcaacat ctaagtttgt
     1441 tggcgaagaa ttagcgaagt ctgaccgtcc agaattaacc gctgctaaga ttgtggtgtc
     1501 tggtggtcgt ggtatgggca atggcgacaa cttcaaaatc ctttacagct tggctgataa
     1561 attgggtgct gctgttggtg catctcgtgc tgctgttgac gctggtttcg taccaaacga
     1621 catgcaagtg ggtcaaacag gtaaaatcgt tgctccacaa ttatatgttg ccgttggtat
     1681 ctctggtgcc atccaacatt tagcaggtat gaaagattct aaagttattg ttgctatcaa
     1741 caaagacgaa gaagctccaa tcttccaagt tgctgactac ggtattgtag gtgacttgtt
     1801 tgatttagtg cctcagttag agaagttagt gtaatcactg ctt
//
LOCUS       DAOQMB010000466         1843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45176_length_1843_cov_13.224273, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000466 DAOQMB010000000
VERSION     DAOQMB010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45176_length_1843_cov_13.224273"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1616)
                     /locus_tag="PLY05_11105"
     CDS             complement(183..1616)
                     /locus_tag="PLY05_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC03887.1"
                     /translation="MLNSEYPYYLANQAVYANQDLAVTNKYTGEIVTRVALATEQAID
                     DALNAAVSAQQAMAQLPAYHRQQVLEHCVQRFKQRADELAYSLCVEAGKPIKDARGEV
                     TRLIDTFKVAAEEATRINGEVLNLEISSRAKGYRGMTKRVPVGVCSFISPFNFPLNLA
                     AHKIAPAIAAGCPFVLKPASLTPIGALLIGEVLAETDLPQGAFSILPCSRDGAELFTT
                     DERIKLLSFTGSPEVGWALKAKAGKKKVTLELGGNAACIVDADADIDFVVQRLVFGAF
                     YQSGQSCIGVQRILIHDSIYELVKQKLVTATQQLVMGDPIQESTFIGPMISDKEAQRL
                     AAWIDDAKQQGATLLCGGQGQGQGAMLAASLLENVPKHCALVQEEVFGPVAVLSSFSD
                     FNQALAEVNESRFGLQTGIFTRDIFKAHQAWDTLEVGGVVIGDVPSWRVDNMPYGGVK
                     DSGLGREGIRYAIEDMTESRLLVIRQP"
     gene            complement(1646..>1843)
                     /locus_tag="PLY05_11110"
     CDS             complement(1646..>1843)
                     /locus_tag="PLY05_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HNC03888.1"
                     /translation="DKLEGGLRVNRLGVSSVEYGVAIFKEGEDVAVADGHFVHVFVDK
                     QSNKSVPIPDMIRKALERIKA"
BASE COUNT          538 a          451 c          315 g          539 t
ORIGIN      
        1 ctcgccagct atcggcagcg ccactttcac catgacattt tgagcagtgt tcttggtata
       61 aggcatatgg ttcaattgga gcagcttgtg ttgtgccgac taaagccgtt aatgccaata
      121 cagcgactaa ttgttgtttt gttatcattg ttatgactct tatttagatt gaagattttt
      181 tattatggtt gtctaatcac caataagcgg ctttcggtca tatcctcaat cgcataacga
      241 atgccttctc gacctaaacc actatcctta acaccaccat agggcatatt gtcaacgcgc
      301 catgagggta catcacctat cactaccccg cctacttcta acgtatccca tgcttgatgt
      361 gctttaaaaa tatcgcgagt aaaaatacct gtttgcaaac caaaacgact ctcattaact
      421 tcggctaaag cttggttaaa atcgctaaaa ctagaaagta ccgcaacagg gccaaacact
      481 tcttcttgaa ctaaagcaca atgtttaggt acattctcta ataagcttgc ggctaacatc
      541 gccccttgac cttgaccttg accaccacac aataatgttg cgccttgttg cttggcatca
      601 tcaatccaag ccgctaaacg ctgcgcttct ttatcgctaa tcattggccc aataaaggta
      661 ctttcttgta tcgggtcacc catgactaat tgctgagtag ctgtaactaa tttttgtttt
      721 accaactcat aaatgctgtc atgaattaaa atacgctgca cgccaataca actttgtccc
      781 gactgataaa aagcaccaaa cactaatcgc tgcacaacaa aatcaatgtc tgcatcagca
      841 tcaacaatac acgccgcatt accgcctaat tctaaggtaa ctttcttttt gccagcttta
      901 gcttttaatg cccaacctac ttcgggtgag cctgtaaaac ttaataattt aattcgctca
      961 tcagtggtaa ataattctgc accatcacga ctacacggta aaattgaaaa tgcaccttga
     1021 ggtaaatcgg tttcggccaa tacttcacca attagcaaag caccaattgg cgttaagcta
     1081 gctggcttca atacaaaggg gcatcctgcg gcaatcgctg gcgcaatttt gtgagcggct
     1141 aaattaaggg gaaaattaaa gggactaata aacgaacaga cccccacagg tacacgttta
     1201 gtcatcccac gataaccttt ggcacgtgaa ctgatttcta aatttaatac ttcgccatta
     1261 attcgagttg cttcttcagc cgccacttta aacgtatcta ttaaacgggt cacttcacca
     1321 cgcgcatctt taatcggttt acctgcttca acacacaaac tataggccaa ctcatcagca
     1381 cgttgtttaa accgctgtac acagtgttct agcacttgct gacgatgata agcaggtaat
     1441 tgcgccattg cctgttgcgc gcttacagcc gcgtttaaag catcatctat ggcttgctct
     1501 gtcgctaagg caactcgtgt aactatttca cctgtatatt tattggtaac tgctaaatct
     1561 tggttagcat aaacggcttg attggccaaa taataaggat actcagagtt aagcatattg
     1621 aatcctaatt tttaatgttg ttgttttagg ctttaattct ttctagagct ttacgaatca
     1681 tatcaggaat aggcactgac ttattacttt gcttatcgac aaaaacatga acaaaatgac
     1741 catccgccac cgccacatct tcaccttctt taaaaatggc tacaccatat tctaccgaag
     1801 atacgcccaa acgattgaca cgcaaaccgc cttctaactt atc
//
LOCUS       DAOQMB010000467         1842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45221_length_1842_cov_14.719082, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000467 DAOQMB010000000
VERSION     DAOQMB010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45221_length_1842_cov_14.719082"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1842)
                     /locus_tag="PLY05_11115"
     CDS             complement(<1..>1842)
                     /locus_tag="PLY05_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03889.1"
                     /translation="SKKEDSIQPTSKTPLLDKNNSFMDLVRDGKATAEQFKQSFDDLF
                     ANKDAVIAELNTLTKDQLLKKGGYNIQYRYKNDKKSEVAEAVYDSMIAKYALGRGISY
                     GMGKNSYENAVKKMVDDTDQARLDDFAKEYKQMIDDHAKKIAEKVEALKNPQTLEDYR
                     NLVRSKMADGSTRQEAFLSLTPEQRIKYDELEAESTKETREAKKRALQSTVNTASQTT
                     TGTIIETKHTRDGYDLFVVQLSDRLSTDDYKQVLSEAKRLGGWYSSFRGNGAIAGFQF
                     KDKGSAEAFLALAGGDTTAAKEQIDAKIDAFEDNRSQTAVERLQTMADKLEEKANEEL
                     ERDRKVNTARRARFAASAEFAARSQIALAKTMRNIADAISSGKAKFLDNIRMKTDVET
                     LQSYIASAKDNELRAKYKTYAEQEKRKGQPATVETADFATYPSYTLYRSDLAMLGRKL
                     LEIEGLKKFGQQIMAVADDVSDAYLEFAKKNLHQVSRFSKSDSSLATFSSKDIAERSI
                     RKSGLTGKAIVLQVKRGENIIILSPSEAINLKVWEGDADKRITLKREFGNDLVNAVGR
                     RAGKNNNLLPYQFQLNYDKFKALSRMGIETPSEFRSALREFIALQEE"
BASE COUNT          446 a          407 c          347 g          642 t
ORIGIN      
        1 cctcttcttg taaggcaata aactctctta atgcgcttct aaattcacta ggcgtttcaa
       61 tacccatgcg tgataatgct ttaaacttgt catagttaag ctggaattga taaggcaata
      121 gattgttatt cttccctgct ctacggccaa cagcattaac caaatcatta ccaaactcac
      181 gctttaaggt aatacgtttg tctgcatcac cctcccagac ttttaagtta attgcttcgc
      241 ttggacttaa aataatgatg ttttcgccac gcttaacctg taaaacaatg gcctttccag
      301 ttaatccgct tttacgaatt gagcgttcag ctatatcttt actagagaat gttgctaaag
      361 atgaatcgct ttttgaaaag cgagatactt ggtgtaagtt ctttttggca aactcaaggt
      421 aagcatcgct cacatcatca gcaacagcca taatctgttg gccaaacttt ttgagacctt
      481 caatctctaa tagttttcgg ccaagcatgg ctaagtctga acgatacaag gtgtaggatg
      541 ggtaagtcgc aaagtcggct gtttctactg ttgctggctg ccctttgcgt ttttcttgct
      601 cggcataggt tttgtacttt gctcgaagtt cgttgtcttt tgcggatgca atgtaagact
      661 gtagcgtctc aacatccgtt ttcatgcgga tgttatctaa aaacttagct tttccactac
      721 tgattgcgtc agcaatatta cgcatggtct tggccaaagc aatttgagag cgtgctgcaa
      781 actctgctga tgctgcaaat cttgctctac gcgctgtatt taccttgcgg tctctttcaa
      841 gttcttcatt ggccttttcc tctaatttgt cggccatagt ttgtaatcgt tcaacggctg
      901 tttggctgcg gttatcttca aaagcatcaa tcttagcgtc tatttgctct tttgctgccg
      961 tggtgtcacc gcctgcaagt gctaaaaacg cctctgctga acctttgtct ttaaattgga
     1021 atccagcaat tgcaccatta cctctaaagc tactgtacca accacctaaa cgcttggctt
     1081 cagataatac ttgtttgtaa tcatcggtgc ttaaacggtc tgataactgc accacaaaca
     1141 agtcatagcc atctcgcgta tgcttggttt cgataatagt tccagttgtg gtctgagatg
     1201 ctgtattaac ggttgattgc aaggcgcgtt tcttagcttc tcgtgtctcc ttggtgcttt
     1261 cggcttccag ttcatcatat ttgattcgct gctcaggcgt taagctcaaa aaagcctctt
     1321 gacgtgtact gccgtctgcc atttttgagc gcactaagtt gcggtaatct tctaatgttt
     1381 gtggattctt taacgcttcg actttttcgg ctatcttctt ggcgtgatca tcaatcattt
     1441 gcttatattc tttagcaaaa tcatctaagc gtgcttggtc agtatcatca accatcttct
     1501 taacagcatt ttcatagcta tttttgccca tgccatagga aattccccga ccaagcgcat
     1561 acttggcaat catggaatca tagacagctt cggctacttc tgatttttta tcgtttttat
     1621 aacgatactg aatgttataa ccgccttttt ttaaaagttg gtcttttgtt aaggtgttta
     1681 attcagcaat aacagcatct ttatttgcaa ataaatcatc aaaagattgc ttaaattgct
     1741 ctgctgttgc cttgccatca cgcaccaaat ccatgaagct gttattttta tcaagcaaag
     1801 gtgttttgct tgttggctgt atagaatctt cttttttgct at
//
LOCUS       DAOQMB010000468         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45604_length_1834_cov_12.811130, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000468 DAOQMB010000000
VERSION     DAOQMB010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45604_length_1834_cov_12.811130"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..641
                     /locus_tag="PLY05_11120"
     CDS             21..641
                     /locus_tag="PLY05_11120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNC03890.1"
                     /translation="MSFYADYIFPVVLDVATKPLKPQRQQTIALAKGRILEIGIGTGA
                     NLPFYGSDASEIIGIEPELPMLKKAELMVKKAPSQAVVKLAVGDAHHLEFADNSFDTV
                     IMCLVLCTIPNPILALQEAYRVLKPNGQLLFLEHVRAPKPWLARCQDAINPAWKHLAC
                     GCQLNRDTEATIKQAGFKFEKIERFQHPKLVSVGGCIIQGVAIKQG"
     gene            647..1027
                     /locus_tag="PLY05_11125"
     CDS             647..1027
                     /locus_tag="PLY05_11125"
                     /inference="COORDINATES: protein motif:HMM:NF040078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HNC03891.1"
                     /translation="MNLEKVVFAFFIVLAATLNFGFFIGDIDDPSLHHLGEFAAALIV
                     NLIATSLKFGDRSHMGATQLATSLVAVLQLVAAAIVWAYQVHVVGDMTPSVMANIVSL
                     SGGAMLANFVSLILLIAETVMFRR"
     gene            1027..>1834
                     /locus_tag="PLY05_11130"
     CDS             1027..>1834
                     /locus_tag="PLY05_11130"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC03892.1"
                     /translation="MNTAFFIALRRLRFPIIALICSYAISVTGLVLIDGTDANGKPTP
                     MSWFHAFYFMSYTATTIGFGEIPYAFTNAQRMWVTFCIYLSVVNWAWLLAAIMALAQD
                     RTFLSAMKRGSFSRQVQRMREPFWLMCGYGQTGALLCDRLGHTGQRVVVLEKREHEIF
                     ELELHDHGISVPALLADARDAEILMAAGLSHPELAGVLALTSDDATNLGIAAAARVLN
                     PYVPVYCRSETPSVSESALAFGTDMLINPFTVFTDELRLLLINPQAHTLFT"
BASE COUNT          470 a          339 c          408 g          617 t
ORIGIN      
        1 cagatttata ggaagttaat atgtcttttt atgccgatta tatttttcct gtggttttag
       61 atgtggcaac caaaccctta aagccacaac gtcagcaaac tattgcctta gccaaaggtc
      121 gaattttaga gattggcatt ggtacaggtg caaatttacc gttttatggc agtgatgcga
      181 gcgaaattat tggcattgaa cctgagttgc caatgctaaa aaaagcagaa ttgatggtca
      241 aaaaagctcc ttcacaagca gtggttaagt tggcagtggg tgatgctcat catttagagt
      301 ttgccgataa cagctttgat acggtgatta tgtgtttggt gttatgcacc attccaaatc
      361 ctatcttagc cttgcaagaa gcctatcgag tcttaaaacc caatggccaa ttattatttt
      421 tagaacatgt acgcgcacca aaaccttggt tagcacgttg ccaagatgcc attaatcctg
      481 catggaaaca tttagcctgt ggttgtcagt taaatcggga tacagaagcc accattaaac
      541 aagcaggctt taaatttgaa aaaatagagc gttttcaaca tcctaaatta gtctcggtcg
      601 gtggttgtat tattcaaggt gtggcgatta aacaaggata aataaaatga atttagaaaa
      661 agtcgttttt gcctttttta ttgttttggc agcaacctta aattttggtt tttttattgg
      721 tgatattgat gatccgtcat tacatcattt aggagagttt gcggctgcct tgattgttaa
      781 tttaattgcc acctcgttaa agtttggcga tcgctcacac atgggcgcaa ctcaattagc
      841 caccagttta gtggctgttt tgcaattggt tgcggcggct attgtatggg catatcaagt
      901 acatgttgtt ggcgatatga ctccaagtgt catggcaaat attgtctcct tatcgggtgg
      961 ggcaatgtta gctaattttg tctcgcttat cttattaatt gccgaaactg tgatgttccg
     1021 tcgctaatga ataccgcctt ttttattgcc ttacgccgtt tacgttttcc gattattgct
     1081 ctcatttgta gttatgcaat tagcgtcaca ggtttagtgt taattgatgg tacagatgcc
     1141 aatggcaaac caactcccat gagttggttt catgcttttt attttatgag ttataccgca
     1201 acaaccattg gttttggtga aattccgtat gctttcacga atgcccaacg tatgtgggtg
     1261 actttttgta tttatttatc ggtggtgaat tgggcgtggt tgttggctgc gattatggct
     1321 ttagctcaag atcgtacttt tttatctgcc atgaagcgtg gtagctttag ccgccaagta
     1381 caaagaatgc gtgagccgtt ttggttaatg tgtggttatg ggcaaacggg cgcattatta
     1441 tgtgatcgtt tgggacatac aggtcaacga gttgttgtgc ttgaaaaacg agagcatgaa
     1501 atttttgaac ttgaattaca tgatcatggc attagtgtac cagctttatt agcagatgcg
     1561 cgtgatgccg aaattttgat ggcagcaggg ttatctcatc ccgaacttgc gggtgtactt
     1621 gctttaacct ctgatgatgc caccaatttg ggtattgccg cagccgcgcg cgtgcttaac
     1681 ccctatgtgc ctgtctattg tcgctccgaa acgcccagtg tttccgaaag tgctttagca
     1741 tttggtacgg atatgctgat taatccattt accgtattta ctgatgaatt acgcttatta
     1801 ttaataaatc cccaagcaca tactttgttt acac
//
LOCUS       DAOQMB010000469         1830 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45779_length_1830_cov_13.839437, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000469 DAOQMB010000000
VERSION     DAOQMB010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45779_length_1830_cov_13.839437"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PLY05_11135"
     CDS             complement(<1..1172)
                     /locus_tag="PLY05_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNC03893.1"
                     /translation="MFKAGWSKREILVKAQGYAMMGYGMWHNRAWGQQTPLFARAIWL
                     ADEQGNEIAFCCLDLGYITYAMRSTIIRQLQDTIGDSFNPERLVLTCTHTHSGPGGCT
                     HDALYNVVTPGFVPDNLQQIVLATVEALLAARTHLQAVELSLAHAAFAETTAVAWNRS
                     LDAYNRNPDVSKLNHQQCHLALNREMPVLAIRHQGQVAAFVSLFGVHATAVGNNQTKY
                     CADNKGYAAVAAEAALTQQGVAAPVAIFAQATAGDVSPHYHGSNDIARRKQWQGEKEY
                     TYAEQNGRLQAEHALQIIQQASLPITGALDAILTYVDLSNQHAEPRFANGEQQAFTSQ
                     PCHGVAFFTGTRVDGPGMPMAIGKVAKQLARAVKTTRLRRLHKLTLAEQQYYQRLYA"
     gene            1370..>1830
                     /locus_tag="PLY05_11140"
     CDS             1370..>1830
                     /locus_tag="PLY05_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC03894.1"
                     /translation="MKDMQFTHKWVLVTGASSGLGLEMATQLADQHQANLILVARREA
                     QLLALKQRLEQSSGIQCHVIAADLSLADDVERVYQQATQLADIYGVILNAGVTHFGEH
                     HELAWPDFEKMLALNVTSVVRLLTLFSQYLKDKNQGGGMMVISSMAGLLPVP"
BASE COUNT          497 a          435 c          380 g          518 t
ORIGIN      
        1 gcatataaac gttgataata ctgctgttct gcaagcgtta atttgtgcaa acgccgcaaa
       61 cgtgtcgtct taaccgcacg cgctagttgc ttggcaactt taccaatagc cataggcata
      121 cccggcccat ccacacgcgt accggtaaaa aatgccacac catgacacgg ctgactagta
      181 aacgcttgct gttcgccatt ggcaaaacga ggttcagcat gttgattgct taaatcgaca
      241 taagtcaaaa tggcatctaa cgcgcctgta atgggtaaac tagcttgttg aataatctgt
      301 aatgcgtgtt cggcttgcag gcgaccattt tgctcggcat aggtatattc tttttctccc
      361 tgccactgct tgcgacgcgc aatatcattt gacccatgat aatggggtga cacatcacct
      421 gctgttgctt gtgcaaaaat tgccacaggc gcagcaacgc cttgttgcgt caatgcggct
      481 tcggcagcca ccgccgcata gcctttgtta tcggcacaat atttggtttg attgttgccc
      541 accgccgttg catgaacccc aaacaacgac acaaacgctg ctacttgtcc ttgatgacga
      601 atcgctaaaa caggcatttc tcggtttaag gctaaatgac attgctgatg atttaatttg
      661 ctcacgtcag gattacgatt atacgcatct aatgatcggt tccatgccac tgctgtcgtt
      721 tcggcaaatg ccgcatgtgc caagctcagc tccacagctt gtaaatgagt gcgtgctgct
      781 aataatgcct caaccgttgc taacacaatt tgctgtaaat tatctggcac aaaaccgggg
      841 gtcaccacat tatacaaggc atcatgagtg catccgcctg gccctgaatg ggtatgcgta
      901 caggttaaga ccaaacgctc tgggttaaag ctatcgccaa ttgtgtcttg gagctgacga
      961 ataattgtgc tgcgcatggc ataagtaatg tagcccaaat ctaaacaaca aaaggctatt
     1021 tcattaccct gttcatctgc caaccaaata gcgcgcgcaa ataacggcgt ttgttgtccc
     1081 catgcacggt tatgccacat accatagccc atcatcgcat agccttgcgc tttgactaaa
     1141 atctcgcgct tactccaacc tgctttaaac atggccaacc ctcaaaacct gaattattat
     1201 cagctttaat agtcgcatat ctctactttt tgtcaatacg tgcacttaac ttctttagat
     1261 aaacaaaaaa tctaatcgtt aaccgtatta tcagattgat ttttagccaa tccagtttta
     1321 acctgatata tattcagctt tataacaaca atagcaaatg gagcttttta tgaaagacat
     1381 gcaatttacc cataaatggg tcttagtgac aggcgcatct tctgggcttg gcttagaaat
     1441 ggccacgcaa cttgccgatc aacatcaggc taacttgatt ttggttgccc gtcgtgaagc
     1501 gcaattattg gctttaaaac aacgcttaga acaaagctca ggtattcaat gtcatgtgat
     1561 tgctgccgat ttatcacttg ctgacgacgt tgaacgtgtt tatcaacaag ccacccaact
     1621 ggctgatatt tatggtgtga ttttgaatgc aggggtgacc cattttggcg aacaccacga
     1681 attagcatgg cctgactttg aaaaaatgct tgcgctaaac gttaccagtg ttgtgcgttt
     1741 acttacttta tttagccaat atctgaaaga caaaaatcaa ggtggaggca tgatggtgat
     1801 tagtagcatg gcaggtttgt tgcctgttcc
//
LOCUS       DAOQMB010000470         1829 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45830_length_1829_cov_10.970688, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000470 DAOQMB010000000
VERSION     DAOQMB010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45830_length_1829_cov_10.970688"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="PLY05_11145"
     CDS             <1..521
                     /locus_tag="PLY05_11145"
                     /inference="COORDINATES: protein motif:HMM:NF015963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HNC03895.1"
                     /translation="WSPSLRRRIAQMVADNHGTEYAVDYAWKLLASERSVRFHEMEYH
                     LPLDAQIPALKEVIEVIEKQRSDVFFPIEARVIKGDDRAWLSPFYQQDTGSIAVHAYY
                     EDDYKFLYSLIEPILKRYGGRPHWGKMHSLADKDFALLYPQWQQVKAIRQQLDPQNKF
                     VNNYTKKIWGLA"
     gene            518..1792
                     /locus_tag="PLY05_11150"
     CDS             518..1792
                     /locus_tag="PLY05_11150"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNC03896.1"
                     /translation="MKLSRRQLMMATGLGAVTAGIAVKPSDNGAPHNSYFMDLTQALK
                     KAALAQPTLIIDKQALDHNIQQVKQATAAHKLPVRLVVKSLPSVDLLNYLSQELNSQR
                     FMVFNLPMLTTVTQAYPQGDFLLGKPLAALAVQEWLRVESNRAALPHIQWLIDSPQRL
                     TEYGEVAKQFNVQLRVSLEIDVGLHRGGFVDVGELKQVVQTIKSNPQLQLAGLMGYEA
                     HVGKLPTILNMQQKAWDHFKNTYQQAISILTEAGYEPQKLTLNAGGSPTYTLHAKGTV
                     ANEVAVGTAFVQPCDFDLATLSQHKAACFIASPVLKALNPALIPAIEWADGLRRWWDV
                     NNEQGYFIHGGNWLAKPESPQGLALSGLYGRSSNQELWLGSSSQHLQVNDYVFFRPQQ
                     SEAVFLQFGDIVVFDNQQKQISQRWPVFSMSA"
BASE COUNT          550 a          341 c          411 g          527 t
ORIGIN      
        1 gttggtcgcc aagtttacgc agacgcatcg cacaaatggt ggccgataat catggtactg
       61 aatacgctgt cgattatgct tggaaattat tagcctcaga acgctcggta cgctttcatg
      121 agatggaata tcatttgcct ttagatgctc aaattcctgc acttaaagaa gtgatagaag
      181 tgattgaaaa gcaacgttct gatgtgtttt ttccaattga ggcacgcgta attaagggtg
      241 atgatcgggc atggttatca ccattttatc agcaagatac aggctcaatt gcggtacacg
      301 cctattatga agatgactac aaatttttat acagtttaat tgaaccgatt ttaaaacgtt
      361 atggtggtcg cccacattgg ggaaaaatgc actcattagc tgacaaagat tttgccttgt
      421 tatatccaca atggcagcaa gtcaaagcca ttcgtcagca attagaccca caaaataaat
      481 tcgttaacaa ttacaccaaa aagatttggg gcttagcatg aagttatctc gccgtcaact
      541 gatgatggct acaggtttag gtgcagtaac agcaggcatt gccgttaagc ccagtgataa
      601 tggtgcgccg cataattcgt attttatgga tttgacacag gcattaaaaa aagcagcttt
      661 agctcaaccc actttaatca tagataaaca agctttagac cataatattc aacaagtgaa
      721 acaagcaaca gccgcacata agctacctgt tcgtttggtg gttaagtcgt taccgtctgt
      781 agatttactc aattatttaa gccaagaatt aaatagccaa cgatttatgg tgtttaattt
      841 accgatgttg acgacagtga ctcaggctta tcctcaaggt gattttttat taggtaagcc
      901 tttagcggca ttagcggtgc aagagtggtt aagagttgag agcaatcgcg cggcattgcc
      961 acacattcaa tggttaattg attcgccaca acgactcact gaatacggtg aagtagccaa
     1021 acagtttaat gtgcaattgc gtgtgagttt agaaattgat gtgggtttgc atcggggcgg
     1081 ttttgttgat gttggtgagc taaaacaagt agtgcaaacc atcaaaagca accctcaatt
     1141 acagcttgca gggttaatgg gttatgaagc acatgtaggc aaattgccaa caattttaaa
     1201 tatgcaacaa aaagcatggg atcattttaa aaacacctat caacaagcga ttagcattct
     1261 caccgaagca gggtatgagc cacaaaaatt gaccttaaat gcgggtggta gtccgactta
     1321 tacgttacat gcaaaaggga cagtggctaa tgaagtagcg gtgggaacgg cgtttgtgca
     1381 accgtgtgat ttcgatttgg caaccttaag tcaacacaaa gccgcctgtt ttattgctag
     1441 ccctgtatta aaagccttaa atcctgcctt aattcctgct attgagtggg cagatggttt
     1501 gcgccgttgg tgggatgtca ataatgagca aggttatttt attcatgggg gtaattggtt
     1561 agccaagcct gaatctccac aaggtttagc gttaagtggt ttatatggcc gttctagtaa
     1621 ccaagaacta tggttaggct catcaagtca gcatttacaa gtaaacgatt atgttttttt
     1681 tcgaccacaa caaagcgagg cggtgttttt gcagtttggc gatattgtgg tctttgataa
     1741 tcaacaaaaa caaataagcc aacgttggcc agtctttagt atgtcggctt aactgtaaat
     1801 aaccgccttt aatctaggtg aggctagta
//
LOCUS       DAOQMB010000471         1828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45880_length_1828_cov_7.771574, whole genome shotgun sequence.
ACCESSION   DAOQMB010000471 DAOQMB010000000
VERSION     DAOQMB010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45880_length_1828_cov_7.771574"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLY05_11155"
     CDS             <1..440
                     /locus_tag="PLY05_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009767170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNC03897.1"
                     /translation="KITAVIDYAHTPDALEKVLLSLREHTSGQLWCVFGCGGDRDKGK
                     RPIMGEIACRLADKVIVTADNPRTESVSAIIADIEAGMGHQQHVVLPERKQAIEYALS
                     AAQAGDIVLIAGKGHEDYQEIQGVRHPFDDAQIVRNCALFHRE"
     gene            525..>1828
                     /gene="murF"
                     /locus_tag="PLY05_11160"
     CDS             525..>1828
                     /gene="murF"
                     /locus_tag="PLY05_11160"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNC03898.1"
                     /translation="MKLSVIAEKTQGQLFGQDLDVCQFSTDTRTLTAGDVFVALSGAN
                     FDANSFVAKAQELGAVAAIVSQYQADISLSQIVVANTQTALGLLAKAWREQFELMRVA
                     MTGSSGKTTTKELTASIFRQAGETLATLGNKNNEIGVPLTLLRLNAQHRYGVFELGAN
                     HIGEIAYTSGLVQPHAAVITNIGTAHLEGFGSRQGIAQAKGEIFSGLVDGGTAVINAD
                     DEFADYHQNLCVGLKILTFSVKKQADAYATDIKRTNGGAYQFNLHLAEQSTPIQLRLI
                     GQHNVANALAASCLALACGLTLPQIKQGLEEAQGAAGRMVLHRCQNFTLIDDTYNANP
                     DAMKAAIDELALSDGYKIIVLGAMGELGDNAPSLHQQVGAYAQAKKIDALYCVGAFSE
                     HTAQGYGTQAQRFSEQKELIDALLLAIPHGATILVKGSRSAR"
BASE COUNT          525 a          330 c          439 g          534 t
ORIGIN      
        1 ataaaattac cgctgtcatt gattatgcac atacacctga tgccttagaa aaagtgttat
       61 taagcctacg agagcatacc agtggtcaat tgtggtgtgt gtttggttgt ggcggtgatc
      121 gagataaagg caaacgtccg attatgggtg aaattgcttg tcgtttggca gataaagtga
      181 ttgtaactgc tgataatcca cgcacagaat cagtcagtgc gattattgct gatattgaag
      241 caggtatggg gcatcagcaa catgtggttt tgcctgagcg taaacaagcg attgagtatg
      301 ccttgtcggc cgcacaagcg ggcgatattg tgctaattgc gggtaaagga catgaggact
      361 atcaagaaat acaaggtgtc cggcatcctt ttgatgatgc tcaaattgtg aggaattgtg
      421 cactcttcca tcgtgaatag agtttgcatt taatgatatt ttaacacgca aatgtaagat
      481 cattgtgcca atttcgaaag ctgtttattt ttagagaaaa attcatgaaa ttaagcgtga
      541 ttgctgaaaa aacgcaaggc cagctttttg gccaagactt agatgtttgc caattttcta
      601 cggatacacg caccttaacc gcaggtgatg tgtttgttgc cttaagtggt gcaaattttg
      661 acgctaatag ttttgttgcg aaggcacaag aattaggtgc cgttgccgcg attgtcagtc
      721 aataccaagc agatatttcc ttaagtcaaa ttgttgttgc gaatacccaa acagcattag
      781 gcttgttggc aaaagcatgg cgtgagcaat ttgaattaat gcgtgtggcc atgacaggta
      841 gcagtggcaa aaccacgacc aaagaattaa ctgccagtat ttttcgtcaa gcaggtgaaa
      901 cgctggcaac tttaggtaat aaaaataacg aaattggcgt gccattaacc ttgttacgcc
      961 taaatgctca gcatcgttat ggcgtgtttg aattaggtgc gaatcatatt ggcgaaattg
     1021 cttatacatc tggtttagta caacctcatg cagcagtgat taccaatatt ggtacagctc
     1081 atttagaagg ttttggtagt cgccaaggga ttgctcaagc taaaggtgaa attttttcgg
     1141 gtttggttga tggtgggaca gcagtaatta atgctgatga tgagtttgct gattatcacc
     1201 aaaacttatg tgttggctta aaaatactca ctttttctgt taaaaaacaa gctgatgcct
     1261 atgcaacgga tattaaacgt acaaacggtg gtgcatatca atttaattta catcttgccg
     1321 aacaatcgac acccattcag ttaaggctaa tcgggcaaca taatgttgct aatgccttgg
     1381 cggccagttg tttggctttg gcttgtggtt taacgttgcc acaaattaaa cagggtttag
     1441 aagaagccca aggtgcagca gggcgcatgg tattgcatcg ttgtcaaaat tttaccctga
     1501 ttgatgatac ctataatgcc aatcccgatg ccatgaaagc agcgattgat gagcttgcct
     1561 taagtgatgg ttataaaatt atcgtcttgg gtgcaatggg tgagttgggt gacaatgccc
     1621 caagtctaca tcagcaagtg ggtgcttatg ctcaagccaa aaaaatcgat gccttgtatt
     1681 gtgtcggtgc atttagtgaa catactgccc aaggttatgg cactcaagca caacgtttta
     1741 gtgaacaaaa agagctgata gacgcattat tgctggctat tcctcatggg gcaaccattt
     1801 tggtgaaagg ttcacgcagc gcacgcat
//
LOCUS       DAOQMB010000472         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45960_length_1826_cov_16.596273, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000472 DAOQMB010000000
VERSION     DAOQMB010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45960_length_1826_cov_16.596273"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="PLY05_11165"
     CDS             complement(<1..251)
                     /locus_tag="PLY05_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03899.1"
                     /translation="MNGLAFFPRLKQVLLGSSFALISGLSNAQSLCVFDVLGMQGPFY
                     GTMREYATAARKWNVELELKAYTSEEKIAEDFSAGRCDIA"
     gene            complement(329..1324)
                     /locus_tag="PLY05_11170"
     CDS             complement(329..1324)
                     /locus_tag="PLY05_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC03900.1"
                     /translation="MNINDLPFLPVVYLSKLTEQMLEENIQPELLLRDCGISPNILKN
                     PEAYLSVYQVRAIVSRYIGLTGLSCAGIRYGQRLDLLTHGLFGYVFLTRLPFREFMTN
                     IFHYLQVRLPLLSLELCQESDYISVKLSYRQPLHEIEAFVTQAFLSSLYNLVSLITKH
                     ISLHFQSHSLNHHAHLHDLLPVPIEIGSAANEIRFYAADHYSQQNKVSSITEQADPFY
                     EHSLVVKMRAYILSRADQALTAEDVAQYLNMSVRTLRRRLADIGMSFNDIRLEVRMQA
                     ATRYLKYSKLSIERIANIVGYSDQASFSRAFQKWANDTPDATRRKHRQQQTLTIP"
     gene            1555..>1826
                     /locus_tag="PLY05_11175"
     CDS             1555..>1826
                     /locus_tag="PLY05_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03901.1"
                     /translation="MKKAIAVPALKAVYCPISKIDVHDIRQMYGIYSQYYERTSWDIF
                     LRDLSKKTGAFLLSCPKEGRIVGFSTILVMDMMVQGEAARGVFSGDT"
BASE COUNT          567 a          325 c          379 g          555 t
ORIGIN      
        1 gcaatatcac aacggccagc actaaaatct tcagcgattt tttcttcgct ggtataggct
       61 tttaattcta attcaacatt ccatttgcgg gcagcagtgg catattcgcg catcgtaccg
      121 taaaacggcc cttgcatgcc taatacatca aatacacata agctttgtgc attactaaga
      181 ccactgatga gggcaaagct actgcccaaa agaacttgtt ttaaacgagg aaaaaaagct
      241 aagccattca tggataacaa ctccaatttt tgttggctga atcatactct gtttagcttt
      301 ttttctccaa atctttgttt aacaactctt agggtatagt cagtgtttgt tgttgacgat
      361 gtttacgacg tgttgcatcg ggcgtgtcgt ttgcccattt ttgaaaagca cgactaaaac
      421 tggcttggtc gctgtagcca acaatattgg caatacgctc aatgcttaat ttggaatatt
      481 ttaaataacg tgttgccgcc tgcattcgca cttctaaacg aatatcatta aagctcatgc
      541 ctatatctgc taaacgtcgg cgtaaggtgc gtacactcat attgaggtat tgggcaacgt
      601 cttcggctgt tagggcttga tcggcacgtg acaaaatata ggcgcgcatt tttaccacca
      661 aactgtgttc ataaaaggga tcggcttgtt cggtgataga agatactttg ttttgctgcg
      721 aataatggtc ggccgcataa aaacgaattt cattggcagc actaccaatt tcgataggca
      781 caggtaataa gtcatgcaaa tgagcatgat gatttaaact atgactttga aaatggagac
      841 ttatatgttt agtaattaaa gaaaccagat tatataaact agataaaaat gcttgagtga
      901 caaaggcttc gatttcatgg agtggttgac gataggatag tttgacagag atatagtcgc
      961 tttcttggca taattctaaa gataataacg gcaaacgtac ttgtaaatag tgaaaaatat
     1021 tggtcataaa ttcacgaaac ggtaaacgag tcaaaaaaac ataaccaaat aagccatgtg
     1081 ttaacaaatc tagtcgttga ccataacgta tgcctgcaca agataatcct gttaggccaa
     1141 tatatcggct aacaatcgcg cgtacttgat aaaccgataa ataggcttca ggatttttta
     1201 gaatattagg gctaatgcca caatcacgta ataacaactc aggttggata ttttcttcga
     1261 gcatttgctc ggttaatttg ctgaggtaaa cgacaggcaa aaaaggtaaa tcatttatat
     1321 tcatagtgtc gaatgtttat tgttttttat ttgcgcttag tgtacgcgcg caataaagat
     1381 agggctagtt tttgttgtcc gcttggccgt ttttgcaaaa caaatggccg tcgcagcgga
     1441 tgactggtag taaaaaggcg atgaaaatat ataaacaata ataaatagtg ttctaacact
     1501 taatacctat ctcgctggac atttaaagtc cacaatcaac aagaagaatt tatcatgaaa
     1561 aaagccattg ctgtacctgc attaaaagct gtttattgcc cgattagcaa aattgatgtc
     1621 catgatattc gccaaatgta tggcatttat agccaatact atgagcgtac ctcttgggat
     1681 attttcttgc gtgatttaag caaaaaaacg ggtgcatttt tattgtcgtg tccaaaagaa
     1741 ggacgaattg ttggtttttc gacgattttg gttatggata tgatggtgca aggcgaggca
     1801 gcgcgtggtg tatttagtgg tgatac
//
LOCUS       DAOQMB010000473         1826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_45961_length_1826_cov_13.516093, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000473 DAOQMB010000000
VERSION     DAOQMB010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45961_length_1826_cov_13.516093"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..628
                     /locus_tag="PLY05_11180"
     CDS             155..628
                     /locus_tag="PLY05_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNC03902.1"
                     /translation="MTNEIRPPVPPFTRETAIQKVRMAEDGWNSRDPQRVSLVYTEDS
                     QWRNRAEFPRGRPQIIEFLTRKWARELEYRLIKELWAFDGNRIAVRFAYEWHDDSGQW
                     YRSYGNENWEFNEQGLMQRRFASINDLPIKASERLFFWPLGRRPDDHPSLSDLNL"
     gene            complement(676..>1826)
                     /gene="nuoN"
                     /locus_tag="PLY05_11185"
     CDS             complement(676..>1826)
                     /gene="nuoN"
                     /locus_tag="PLY05_11185"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HNC03903.1"
                     /translation="MYLLLLIATAGALVLVCSRHMASFFIGLELMSVPVYGMVAYTHH
                     RSRSLESGIKYMVLSATASAFMLFGMALIYAQTGTLAFSGVSEQLGGGAPIGHLATFG
                     VGMIVIAICFKLSLAPFHLWTPDVYQGSPAPVGAFLATIGKVSVFAVLVRFLVETPFE
                     FTLPLREVFSVIAVLSILLGNLLALMQTNLKRLLGYSSIAHLGYLLVALASGGGTALE
                     TVHVYLLTYVLTSLGAFGVVTLMSQGKDDEDADSLHHYRGLFWRRPYLTAVMTVMMLS
                     LAGIPLTAGFIGKFYVMLTGVNTGNWWLLGVVILGSAIGLYYYLRVMVTLYMAASGMP
                     KHDAANDWGQRTGGIMVLGVAGLVLLLGMYPQPAIEVIRMAKLLILPH"
BASE COUNT          622 a          408 c          350 g          446 t
ORIGIN      
        1 actctggtgg tgctgaacta tttaaacctt aaacaaaata ggactgacgc atttccccga
       61 aattagcgcg ttttgtgagc ataaaatcgt taaaaacgat ttaaatgcga gcaaataagc
      121 gataaccgcg taagtcctaa aaaggaatac gataatgact aacgaaattc gtccacctgt
      181 tcctcctttt acccgtgaaa cagccatcca aaaagtacgg atggctgaag atggttggaa
      241 tagtcgtgat cctcaacgtg tgtctttggt gtataccgaa gatagtcaat ggcgtaaccg
      301 tgctgaattt ccgcgtggtc gaccacaaat tattgagttt ttaacccgta aatgggcgcg
      361 tgaactggaa tatcgcttaa ttaaagaatt atgggcattt gatggcaatc gtattgcggt
      421 acgttttgcc tatgaatggc atgatgatag tggtcagtgg tatcgctcat atggcaatga
      481 aaattgggaa tttaatgaac aaggcttaat gcagcgtcgt tttgctagca ttaatgattt
      541 gcccattaaa gcgtccgagc gtttattctt ttggccatta ggccgtcgtc ctgacgatca
      601 tcctagttta agtgatttaa atctttaaac aaaaaagcct tgtctttcga caaggctttt
      661 agattactct gctaatcagt gcggtaaaat caacaactta gccatacgaa ttacttcgat
      721 agctggttgt ggatacatac ctaacaataa cactaaacca gcaacaccta ataccataat
      781 accgccagta cgttgtcccc aatcgttagc cgcatcatgt ttaggcatac cagaagcagc
      841 catataaagg gtgaccatga cgcgtaaata ataatacaag ccaatcgcac tacctaaaat
      901 caccacgcct aacaaccacc agttgccagt attcacaccg gttaacatca cataaaactt
      961 accaataaag cctgcggtta acggaatacc tgctaaagac aacatcatca ctgtcatcac
     1021 tgcggttaaa taaggacgac gccaaaataa accacgatag tgatgtaagc tgtcagcatc
     1081 ttcgtcgtct ttaccttgtg acattaaagt caccacaccg aaagcaccta aagaggttaa
     1141 cacgtaagtt aacaagtaaa catgtaccgt ttctaacgct gtaccaccac cagacgctaa
     1201 agccaccaat aagtaaccta agtgagcaat tgaggaataa cccaataaac gctttaagtt
     1261 agtttgcatt aatgccaata agttacccaa taaaatggat aaaacggcaa ttacgctaaa
     1321 cacttcgcgt aatggcaagg taaattcaaa cggtgtttca actaaaaagc gaaccaacac
     1381 cgcaaataca gacactttac caatggtagc taaaaacgca ccaacaggtg ctggtgagcc
     1441 ttgataaaca tcgggtgtcc ataaatggaa cggtgctaaa gacaatttaa aacaaatcgc
     1501 aatcacaatc atgccaacgc caaaagtggc taaatggccg atgggtgcac caccgcctaa
     1561 ttgttcgctg acacctgaga aggctaatgt gcctgtttgg gcataaatta acgccatacc
     1621 aaacaacata aaagcagaag ctgttgcgga taacaccatg tatttgatgc cactttctaa
     1681 ggagcgacta cgatgatgag tataagcaac catgccatac acaggcacag acattaactc
     1741 taaaccaata aagaatgagg ccatgtgacg gctacaaacc aataccaaag cacctgcggt
     1801 tgcaatcaac aacaacaaat acattt
//
LOCUS       DAOQMB010000474         1820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_46211_length_1820_cov_5.627762, whole genome shotgun sequence.
ACCESSION   DAOQMB010000474 DAOQMB010000000
VERSION     DAOQMB010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46211_length_1820_cov_5.627762"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PLY05_11190"
     CDS             <1..591
                     /locus_tag="PLY05_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobile mystery protein B"
                     /protein_id="HNC03904.1"
                     /translation="PLRLRPQYALGATPLDPDEAQGLIPRHITTQGELNEWEQQNITQ
                     GMMWAYGRRNPDLLSEKFLVDLHKKMLSETWSWAGTFRCSDKNIGPAWHTVPVQLKHL
                     LDDVRYQMEHNSFSCDELVARFHHRLVLIHPFPNGNGRHARLMADLLIHQLGGNPFTW
                     GGSSLDKASEIRTRYINALRAADKGDIVPLVLFMRS"
     gene            complement(834..1055)
                     /locus_tag="PLY05_11195"
     CDS             complement(834..1055)
                     /locus_tag="PLY05_11195"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNC03905.1"
                     /translation="MSTLTIRLPDDKHERLKALAVARHISVNKLIDELATIALVEHDA
                     HLRFMVRAKRGNAQQGLALLEVLTQREHP"
     gene            complement(1052..1486)
                     /locus_tag="PLY05_11200"
     CDS             complement(1052..1486)
                     /locus_tag="PLY05_11200"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNC03906.1"
                     /translation="MKKVVIDTNVIVSAIQTGDSISQDLLRLVFLGNITILMSNALFL
                     EYEAISQRHDVMARCRFTQSEAEQFLDALFAVSYWVEIYFAWRPNLKDENDNFLIELA
                     VAGNADYLITKNIKDLKSGELLFPQLSICSPQQFMANWRSQT"
BASE COUNT          512 a          386 c          377 g          545 t
ORIGIN      
        1 ccactgcgcc tcagacccca atatgctctc ggtgcaactc cgcttgatcc tgatgaggcg
       61 caaggactta ttccgcgtca tataaccact caaggcgagc tgaatgaatg ggagcagcaa
      121 aacatcacgc aaggcatgat gtgggcgtat ggacggcgta atcctgattt gttatcagaa
      181 aaatttttgg ttgatttgca caaaaagatg ctgagtgaaa cgtggagctg ggcaggtact
      241 tttcgatgtt cggataaaaa tatcggccct gcttggcata cggttcctgt acagttaaag
      301 cacttgctgg atgatgtgcg ttatcagatg gaacacaact cgttttcttg tgatgagctg
      361 gttgcccgtt ttcatcatcg tttagtgctg atacatccgt ttccaaatgg caatggccga
      421 cacgctcgat tgatggctga tttactgatt catcaacttg gtggaaatcc ttttacatgg
      481 gggggtagct cattggataa ggccagcgaa atcagaacac gctatatcaa tgcgcttaga
      541 gcggctgata aaggggatat tgttcctctg gttttattta tgcggagcta gatgagtaaa
      601 tactcaattg agtatgggct tatgatgttt taactgtatg cctaaaataa agttgtaacc
      661 tgataagtat ttttagggtc aatctcatgg tattacaaaa agttttaaaa cctacgccct
      721 tgcacttact tgtcctccct ttaaaaatgg ggaggcgcac agtaccttgt tagcgatgat
      781 acctcctcct ttaaaaagga agatttaccg ccttgttccg caatccttgc aggctacgga
      841 tgctctcgct gagtcaacac ttctaacaaa gccaaacctt gttgagcatt gcctcgcttg
      901 gctcgaacca taaaccgcaa atgggcatca tgctctacca acgctatcgt ggccaattca
      961 tcaatcaatt tattgacgct aatgtgacga gcaacagcca atgctttaag ccgctcatgt
     1021 ttgtcatcag gtaaacgaat ggtgagtgta ctcatgtttg actccgccaa tttgccataa
     1081 attgttgtgg gctacagata gataattgcg gaaataataa ttcaccactt tttaggtctt
     1141 tgatattttt agtaattaaa taatccgcat tacctgctac cgccaattca atcaaaaaat
     1201 tatcattttc atctttgagg ttgggtcgcc atgcaaaata aatttctacc caataagaca
     1261 ctgcaaatag cgcatctaaa aattgttctg cttctgattg ggtaaaccga catcgcgcca
     1321 taacgtcatg tcgttgacta attgcttcgt attccaaaaa caaggcgttt gacatgagta
     1381 tggttatgtt acccaaaaat acaaggcgca acaaatcttg gcttatgctg tcgcctgttt
     1441 gaatagcact cacaatgaca ttggtatcaa taacaacttt tttcatgttt atttgatagc
     1501 atataagctg tcatgtgtaa agtttatgcc ataaaaaaga cgcaatcaag cgtctttttt
     1561 aggtttagta gcccgacaac actcacgcta atactgcaaa ttgctgtttt ggtgatgggt
     1621 cgcccccgca atccttgcga gctacatcac tgttttataa agcatttatc ctgaatttcg
     1681 ctacgctgca tacaggctac ggcattagca attatgcccc ctcctttatt aaagaagggt
     1741 tggggaggat taattaaaat caatgtacat cactgtacta ggacttaaat tagcctccga
     1801 cagatttgcc gctatataat
//
LOCUS       DAOQMB010000475         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_46312_length_1818_cov_5.385706, whole genome shotgun sequence.
ACCESSION   DAOQMB010000475 DAOQMB010000000
VERSION     DAOQMB010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46312_length_1818_cov_5.385706"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLY05_11205"
     CDS             <1..548
                     /locus_tag="PLY05_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03907.1"
                     /translation="QKERENNPNGTSIYDSLQLKPKKSTAVLDSLRLPEKTANDCKNV
                     VFDLDKGTINGIKPTVSQDDVRAALPCFTGSTPDGDRLNGMLYNYGGGVFFNNHNFYF
                     YTGLDFIEVRRDFTGTVSPNLLGASKERLTGAYGRPTLTSNGSYFYMMSYGCLQFELR
                     NNMVYEIRSHYQDCKAASEVR"
     gene            550..798
                     /locus_tag="PLY05_11210"
     CDS             550..798
                     /locus_tag="PLY05_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03908.1"
                     /translation="MVSSEITLYELISLAFIEIREASISGNLKKSFALSDLFHNVPSH
                     LIQVKLNQGTYDEVFNTLKIRAEMKNLSSWFENAVQQL"
     gene            987..1295
                     /locus_tag="PLY05_11215"
     CDS             987..1295
                     /locus_tag="PLY05_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNC03909.1"
                     /translation="MRVIARRSLCQFWQKTGKEDAKTPLELWYHDAIKADWRTPHDIK
                     QYAANASICGNNRVVFNIGGNKYRLVVEVQYQAAIVWVKFVGTHAEYDKINVETVNEY
                     "
     gene            1285..1641
                     /locus_tag="PLY05_11220"
     CDS             1285..1641
                     /locus_tag="PLY05_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010319432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC03910.1"
                     /translation="MNIKPIRNDDDLTAAFIRLESIFQAIDGSPEADERDILVTLIEA
                     YENQYYPIHQPDPIEAIKYRMLEKGLTNKDLEPYLGGQSRVSEVLNRKRGLSLKMMKR
                     LHDGLNISYETLMIGV"
BASE COUNT          580 a          314 c          369 g          555 t
ORIGIN      
        1 tgcaaaaaga gcgcgaaaac aatcctaatg gcacgagtat ttatgactct ttgcagctaa
       61 aaccgaaaaa aagcacagcc gttttggact ctttacgctt gcctgagaaa accgctaatg
      121 actgcaaaaa cgtggtgttt gatttagata aaggcactat taacggcatt aagcctaccg
      181 tcagccaaga tgatgttagg gcagccctac cctgttttac aggtagtacg cctgatggtg
      241 accgtttaaa tggcatgttg tataactatg gtggtggtgt gttttttaat aaccataact
      301 tttattttta tactggctta gattttattg aggtgcgtcg tgattttaca ggcactgtta
      361 gccccaattt gttgggagca tctaaagagc gtttaactgg cgcttatggc cgccccactt
      421 taactagcaa tggtagttat ttttatatga tgagttatgg ttgtttgcag tttgaactac
      481 gcaataatat ggtttatgaa attcgtagcc actatcagga ttgtaaggca gcaagtgagg
      541 tgcggtgaag tggtatctag tgaaataact ctctatgaat tgatatcttt agcctttatc
      601 gaaattagag aagcatcaat tagtgggaac ttaaaaaaat cttttgcttt gtcagattta
      661 ttccacaatg ttccatcaca tttaatacaa gttaaactca atcaaggaac ttatgatgag
      721 gtttttaata cattaaaaat aagggctgaa atgaagaact tatcatcttg gtttgaaaat
      781 gctgtacaac aactttagag gtagtgtggg ttgacgaagg aaacccacga tttattagcc
      841 aaatcaacat gatgggtttc gttcctcaac ccatcctacc tactatcagc taacaaaata
      901 aagaacctct tcatcaagcc atatcgtata ttgcaaaaat ccatttttgg gataaactac
      961 tactaaagac accatagaga acatttatgc gcgttattgc acgacgctct ttatgccaat
     1021 tttggcaaaa aacaggcaaa gaagatgcta aaactccttt agaactatgg tatcacgatg
     1081 caataaaagc agattggcgt actccgcatg atataaaaca atatgcggca aatgccagta
     1141 tttgtggcaa taacagagtt gtttttaata ttggtggcaa taaatatcgc ctagtggttg
     1201 aagtgcaata tcaagctgct attgtttggg ttaaatttgt tggtactcat gcagagtacg
     1261 acaaaattaa tgtggagacg gtcaatgaat attaaaccta ttcgtaatga tgatgactta
     1321 acagcggctt ttattcgttt agagtctatt tttcaagcta tagatggcag tcctgaagca
     1381 gatgaacgag atattttagt cacactgata gaggcgtatg aaaatcaata ttatcctatt
     1441 catcaacccg accccataga agctattaaa tatagaatgc ttgaaaaagg gttaaccaat
     1501 aaagatttag agccttattt gggtgggcaa agtcgagtgt cagaagtgtt aaatcgtaaa
     1561 cgtggtctga gtttaaagat gatgaaacgt ctgcatgatg gtcttaatat ttcttatgaa
     1621 acattaatga taggtgttta attattcttt tgctcctgcg tagcgagtag cctgtatgca
     1681 gtaaggcagc gtggattgac gaaggaaacc tacattttta ttaaccaaat caacatgatg
     1741 ggttttgtga atcatccctc caaccttaac aacgctttta acgctttggc gcgactcacc
     1801 aataacgaca acaaaatc
//
LOCUS       DAOQMB010000476         1807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_46895_length_1807_cov_11.414384, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000476 DAOQMB010000000
VERSION     DAOQMB010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46895_length_1807_cov_11.414384"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PLY05_11225"
     CDS             complement(<1..343)
                     /locus_tag="PLY05_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019767955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNC03911.1"
                     /translation="MKFCSACGHAVVQKIPQGDTRLRYVCESCETIHYQNPHIICGCL
                     PTWQDKVLLCKRGIEPRYGLWTLPAGFMENLETTQEGAARETWEEAHAKVKIGELYCV
                     FNIPQISQVYMM"
     gene            456..1031
                     /locus_tag="PLY05_11230"
     CDS             456..1031
                     /locus_tag="PLY05_11230"
                     /inference="COORDINATES: protein motif:HMM:NF007980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNC03912.1"
                     /translation="MLQQLLQRLPNDSAQHKADAAVLMAMTREDIPRLILTQRAAHLK
                     SHAGEVAFPGGKRDKTDSSLIYTALREAQEEIALNPQDVEVVGELGIFTSRVGIKVKP
                     IIGLLDDIPHLTPSPDEIDSIFTVPLEVFLQQKPNYQHKIKYMGLSIPVPSFNHEGYV
                     IWGLTGFMIVEFMRQVYDADIDWRWPNPLKR"
     gene            1157..1684
                     /locus_tag="PLY05_11235"
     CDS             1157..1684
                     /locus_tag="PLY05_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004914404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNC03913.1"
                     /translation="MIYSLGDRQLQTHGDYWIADNATVIGSVVLENNVSIWFNVVIRA
                     DNDTISIGENSNIQDGSVLHTDSGVPLTIGKNVTVGHKAMLHGCTIGDNTLIGINAVV
                     LNHAKIGKNCIIGANALVTERQEIPDNSLVMGSPAKVVKTISEGQAMMLQMSALHYVE
                     NFKRYQTQLKPHSAH"
BASE COUNT          545 a          372 c          361 g          529 t
ORIGIN      
        1 acatcatata cacttggcta atttgtggaa tattaaacac acaatacaac tcaccgattt
       61 tgactttagc gtgtgcttct tcccatgtct cacgtgccgc gccttcttga gtcgtttcta
      121 aattttccat aaagccagca ggtaatgtcc acaaaccgta acgaggttct ataccacgtt
      181 tgcataacaa cactttatct tgccatgtcg gtaaacaccc gcaaatgata tgtggatttt
      241 gatagtgaat cgtttcgcaa ctttcacaca cataacgcaa acgagtatcc ccttgaggaa
      301 tcttttgcac aaccgcatga ccacaagcac tacaaaattt cattgtcata tccacgaatt
      361 tattgaatgg tgcatactct agcgagaaaa aacttgtctg cctactcaca aaagccgtac
      421 tattgttaaa agtatcgaat tagaacgaaa gtcccatgtt gcaacaacta ttacagcgtt
      481 tacccaacga tagcgcacag cacaaagctg atgcagcggt attaatggca atgactcgcg
      541 aagacattcc acgactcatt ttaacgcaac gtgctgccca tttaaaaagt catgcagggg
      601 aggtcgcttt tccaggtgga aaacgcgata agaccgatag ttctttaatt tatactgcat
      661 tacgtgaagc acaagaagaa attgccctaa accctcaaga tgtagaagtt gtcggtgaat
      721 tgggtatttt tacctcacgt gttggcatca aagtaaaacc aattattggt ttgttagatg
      781 atattccgca cttaacgcca agccctgacg aaattgacag tatttttacc gtgcctttag
      841 aagtcttttt gcaacaaaaa cccaattatc aacacaaaat caagtatatg gggttaagta
      901 ttcctgtgcc aagttttaat cacgaaggtt atgtaatttg gggattaaca ggctttatga
      961 ttgttgagtt tatgcgccaa gtttatgatg ccgatattga ttggcgttgg ccaaatcctt
     1021 taaagcgtta gcaagttttc cctttggtag ctcctcctcc ttatcaaggg ggaggttggg
     1081 agggggtatt ttagacctca ccctaaccct cccctaaatt aggggagggg aaatagctta
     1141 taatgagagt aataaaatga tttacagttt aggcgaccgt caattacaaa cccatggcga
     1201 ctattggatt gcggataatg ccactgtcat tggctcggtt gttttagaaa ataatgtgag
     1261 catttggttt aatgtggtga ttcgtgccga caatgacacg attagcattg gcgaaaatag
     1321 caatattcaa gatggttcag tgttgcatac cgatagtggc gtgccattaa ccattggtaa
     1381 aaatgtcacg gttggccata aagccatgct acatggttgc acgattggcg ataatacctt
     1441 gattggcatt aatgctgtgg tgctaaatca tgccaaaatt ggtaaaaact gtattattgg
     1501 cgcaaatgct ttagtcactg aacgccaaga aattcctgat aattctttag tgatgggttc
     1561 gcctgcaaaa gtagtcaaaa ccatttccga aggccaagcg atgatgttgc aaatgagtgc
     1621 tttgcactac gttgaaaact ttaagcgtta ccaaactcaa ctcaaaccgc attctgcaca
     1681 ctgagcgaag ttgaagtggt ttcgactccg ctcaaccaac attatttttt tataagacca
     1741 aaatacaaat ctaggttttt tatgtctaaa taccaagctt tacctctaaa aactcgttta
     1801 ttagctg
//
LOCUS       DAOQMB010000477         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_47556_length_1794_cov_3.700403, whole genome shotgun sequence.
ACCESSION   DAOQMB010000477 DAOQMB010000000
VERSION     DAOQMB010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47556_length_1794_cov_3.700403"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..1099)
                     /locus_tag="PLY05_11240"
     CDS             complement(65..1099)
                     /locus_tag="PLY05_11240"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HNC03914.1"
                     /translation="MRAMQLHQTAVITADHPPLQLVDVPVPMPTANQVLLKVEACAVC
                     HTELDEIEGRTAPPQLPIILGHQIVGTVEQIGVNVSSLTKGKRVGVGWIFSACGHCHY
                     CQQGQDNLCQQFKATGRDVNGGYAEYVAVDADFVVSIPDSYTPEQAAPLLCGGAIGYR
                     SLRLANLENGQVLGLSGFGGSNHLVLQLVKYLYPQSAVYVFARSVNEQQFALQLGATW
                     AGDYNQTPPTAMNAIIDTTPVWQPILSLLQHLAAGGRMVINAIRKENTDINAWLALDY
                     ARDLWHEKEIKSVANVASQDITEFLPLAAQAGIKPEIQCYALEQANQALIELKTGQIR
                     GTKVLKVNLS"
     gene            complement(1111..1510)
                     /locus_tag="PLY05_11245"
                     /pseudo
     CDS             complement(1111..1510)
                     /locus_tag="PLY05_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019385310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(1510..1680)
                     /locus_tag="PLY05_11250"
     CDS             complement(1510..1680)
                     /locus_tag="PLY05_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNC03915.1"
                     /translation="MNKKVEAYGVAAVERPKIKVTKQLDLSGEAGRQIVKSETKLALR
                     THVKTFKKLADM"
BASE COUNT          543 a          414 c          300 g          537 t
ORIGIN      
        1 atcatcttag gacttaggca gttatcacat taaccaaatt ttgttaattg catttgactt
       61 aagcctaact caaattcact tttagaactt ttgtgcctct gatttgtcct gtttttaatt
      121 caattaaggc ttggttggct tgctctaaag cataacattg gatttcgggc ttaatgcctg
      181 cttgtgctgc taacggtaaa aactcagtaa tatcctgact ggcaacatta gccaccgatt
      241 taatctcttt ttcgtgccac aaatcacggg cataatcgag agccagccat gcatttatgt
      301 cagtgttttc tttgcgaatg gcattaatca ccatccgtcc gcctgcggct aaatgttgta
      361 acaaacttaa aattggttgc caaacaggag tagtgtcaat aatcgcattc attgcagttg
      421 gcggagtttg attgtaatca cccgcccatg ttgcccctaa ctgcaaagca aactgttgct
      481 cattgactga acgcgcaaaa acataaacag ctgattgtgg atagagatat ttgactagtt
      541 gcaacaccaa atgattagag ccaccaaagc cagataatcc taaaacttgg ccattttcta
      601 gattagccaa gcgtaaactt cgatagccaa ttgccccgcc acacaataaa ggtgctgctt
      661 gttcaggcgt gtagctatct ggaatcgaca caacaaaatc agcatcaact gcaacatatt
      721 cagcatatcc gccattgacg tctcgtcctg ttgctttaaa ttgttggcat aggttgtctt
      781 gaccttgttg acaataatga caatggccac aagccgaaaa tatccaaccc acccccacac
      841 gcttgccttt ggttaatgaa gaaacattta cacctatttg ctcaacagtg ccgacaattt
      901 gatggcctag aataatgggc aattggggcg gtgctgttcg gccttcaatt tcgtctagct
      961 cagtatgaca cactgcacac gcttcaactt tgagtaacac ttgattagct gttggcatag
     1021 gaacaggcac atctaccagt tgtaatggcg gatggtcagc agtgatgaca gccgtttggt
     1081 gaagttgcat agcacgcata atagtgtcct tcatacctta ccctgtaaat aacaagcata
     1141 cagtacatca gcaaaatcac cctctgaaat ctcaccaatt actaaatctc taacagcatc
     1201 tgctaacaaa tcattatcta ataccagttc ataatcattc aaagataaaa actctagtgc
     1261 aactgccaat cctgttcgtt tgttagcatc aggtaatgca tgaactagtg ctatacatct
     1321 atagatgctg tgtatttagc agcaatctca aaaatatcat ctaaaccttc ataaacaatc
     1381 gcattatcaa tacgcgccaa cgctccttgt aattttccta catctgccga gcctttcatg
     1441 cccgctcagt gtccaaaata aaagcattga tttcaataac acgctcaaaa ggaaaacaaa
     1501 taatattcat cacatatcag ctaatttttt aaatgttttt acatgtgtac gtaatgctaa
     1561 tttagtttct gacttaacaa tttgtcgccc tgcctcgcca gacaaatcta actgtttagt
     1621 cactttaatt ttagggcgtt caaccgcagc tacgccataa gcctctactt ttttattcat
     1681 catttactcc gtttaactca ttggattact ttaagcctaa tctcaaaacc ttaaaacaaa
     1741 aagccgttcc ataaaactta tcaaaaccta cttattgtag aacaaatagg cttt
//
LOCUS       DAOQMB010000478         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_47585_length_1793_cov_14.802071, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000478 DAOQMB010000000
VERSION     DAOQMB010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47585_length_1793_cov_14.802071"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..552)
                     /locus_tag="PLY05_11255"
     CDS             complement(202..552)
                     /locus_tag="PLY05_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019838202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HNC03916.1"
                     /translation="MIKRLQVATRYSEAAIHNGVVYLAGQVPNDVTQGIEGQTAEVLA
                     MIDDLLAQAGSSKDHLLTVTIYLTDMADYAGMNKVWDAWLNQGFAPPRACVQAALANP
                     LWKVEIVVVAAQLR"
     gene            678..1202
                     /gene="hslV"
                     /locus_tag="PLY05_11260"
     CDS             678..1202
                     /gene="hslV"
                     /locus_tag="PLY05_11260"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNC03917.1"
                     /translation="MTTIVCVRRNNSVALGGDGQVSLGNTVIKGTARKVRRLYHNQVL
                     AGFAGGTADAFTLFDHFESKLEKHGGNLIRSAVELAKDWRTDRTLRRLEAMLIVADKN
                     ASFIITGTGDVLEPEHGVLAIGSGGNYALAAARALSDNTDLDAEAIAKKSLQIAGDIC
                     VYSNHNHIIEVLKF"
     gene            1213..>1793
                     /locus_tag="PLY05_11265"
     CDS             1213..>1793
                     /locus_tag="PLY05_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC03918.1"
                     /translation="MSTMTPREIVHELDRHIVGQHAAKRAVALALRNRWRRMQLNETQ
                     RHEITPKNILMIGPTGVGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVETIIRD
                     LMEVAIKNERLSAMEKTDYRAEEAAEERILDALLRPARTGSDEWDKDVVQTSEKTDNA
                     TRQLFRRKLQAGELDDREIEIEISQASIGVDIMA"
BASE COUNT          547 a          392 c          353 g          501 t
ORIGIN      
        1 tgtgctacga tagtacaaag aaatctaaag ctctttttga acaaaaattt tggctgtttg
       61 ggttacggta acaggcacag taactgtatt ttgtaacaaa ataaaacata gtaatttcaa
      121 taaattagag ggcatttcga ttgtcgccat cccaatcaaa cccttgaaaa gcctataaaa
      181 atctaggctt tttgttgata tttatctaag ctgcgccgcc accaccacta tttccacttt
      241 ccacaatgga tttgccaaag ccgcttgcac acaagcacgt ggtggtgcaa atccttgatt
      301 taaccacgca tcccacacct tattcatgcc tgcataatcg gccatatccg ttaaataaat
      361 agtcactgtt aacaaatgat ctttactact tcctgcttgc gccaataaat catcaatcat
      421 tgccaagact tcggcagttt gtccctctat cccctgagta acatcattcg gcacttgtcc
      481 tgctaaataa accaccccat tatgaatggc tgcctcacta taacgtgtgg ccacttgtaa
      541 acgtttaatc atcttttcac ccttgccaat tagcccacaa attacacata aaacagcata
      601 aagccttaag ctttatattg aaatacctca aattgtccct atcttagtag aaatatcttg
      661 ctttaatagg aacagtcatg acaaccattg tctgtgtacg ccgtaacaat agcgtcgcgt
      721 tgggcggaga cggccaagtc tctttaggaa ataccgtgat taaaggaact gcacgaaaag
      781 ttcgccgttt gtatcataat caagttttag caggttttgc aggtggcaca gctgatgctt
      841 ttaccttatt tgatcatttt gaatcaaaac tcgaaaaaca tggtggaaac ttaatccgtt
      901 ctgcggttga attagccaaa gactggcgta ccgaccgcac cttacgtcgt ttagaagcga
      961 tgttaattgt tgccgacaaa aatgcctctt ttattattac aggaacaggc gatgtgttag
     1021 aacctgagca tggagtgtta gccattggtt cagggggcaa ttatgcctta gcggctgccc
     1081 gcgctttaag tgataatacc gatttagatg cagaagcaat tgccaaaaaa agcctacaaa
     1141 ttgctggcga tatttgtgtt tatagcaacc ataatcacat tattgaagtg ttaaagtttt
     1201 aaggaagcaa ccatgagtac gatgactccg cgcgaaattg ttcacgaatt agatcgccac
     1261 attgtcggtc aacatgctgc taaacgtgct gttgcccttg ctttacgtaa ccgctggcgc
     1321 cgtatgcaat taaatgaaac ccaacgccat gaaattacgc ccaaaaatat tttaatgatt
     1381 ggcccaactg gcgtgggtaa aacagaaatt gcacgtcgtt tagcaaaatt agccaatgca
     1441 ccttttttaa aagtcgaagc caccaaattt accgaagtgg gttatgttgg tcgtgatgtt
     1501 gaaaccatta tcagagactt aatggaagtt gcgattaaaa acgaacgttt atccgccatg
     1561 gaaaaaaccg actatcgtgc cgaagaagcc gccgaagagc gtattttaga tgccctattg
     1621 cgccctgctc gcactggatc ggatgaatgg gataaagacg tggtgcaaac ctctgaaaaa
     1681 accgataatg ccacacgcca attatttaga cgcaaattac aagcaggtga gttagacgac
     1741 cgtgaaatcg aaatcgaaat ttcacaagcg tctattggtg ttgatattat ggc
//
LOCUS       DAOQMB010000479         1781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_48232_length_1781_cov_15.015643, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000479 DAOQMB010000000
VERSION     DAOQMB010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48232_length_1781_cov_15.015643"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..1417)
                     /locus_tag="PLY05_11270"
     CDS             complement(20..1417)
                     /locus_tag="PLY05_11270"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005240885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HNC03919.1"
                     /translation="MSDRYQLFANSPVGRLLTRNLGLPAPLVLERYDANKPLVSGAVL
                     LGAAPNAELVAPIARVLKNIHAEAYAGNQDFIHQTAASEAGLSLRSHNVEDKEFKFKA
                     FVFDASGVKNSEQLIEVYNFFNPIARQLQNCGRVVILGRTPESCTDPKQATAQRALEG
                     FVRAVAKEFKKAMTAQLVYVAPNAENQVESTLRFFLSAKSAYVSAQVVRISASDTQVN
                     IDWKKPLAGKTALVTGASRGIGEAIAEVLARDGAHVICLDIPAQEADLQRVAGRLGGS
                     VLGLDITAAEAPQKIVDFCKDKGLDIIVHNAGVTRDKTLANMTPQQWNLVMNINLSSE
                     ERVNDALLANNVINANGRVVCVSSISGIAGNLGQTNYAMSKAGVIGMVQSMAPVLKAQ
                     GITINAVAPGFIETQMTAAIPFAIREAGRRMNSMSQGGQPVDVAEAIAWYANPASAGV
                     IGNVVRVCGQSLIGA"
BASE COUNT          527 a          381 c          375 g          498 t
ORIGIN      
        1 tagttgagcg aagtcgaaac taagcaccaa tcaaactttg accacaaaca cgtaccacgt
       61 tgccaattac gccagcagag gctgggttgg cataccaagc aatagcttca gccacatcaa
      121 caggttgacc accttgagac atagaattca tgcggcggcc agcttcgcga atcgcaaaag
      181 gaatcgcagc agtcatttgt gtttcgatga aacccggtgc aactgcatta atggtaatac
      241 cttgtgcttt taagactgga gccatagatt gaaccatacc aatcacgcct gctttagaca
      301 ttgcgtagtt ggtttgacct aagttacctg caataccact gattgaggat acacaaacca
      361 cacggccatt ggcattaatc acattgttgg ctaatagggc atcgttgaca cgctcttcgc
      421 tcgacaagtt gatgttcatg actaagttcc attgttgtgg tgtcatgttg gctagggttt
      481 tgtcacgtgt tacgcctgca ttgtgaacaa tgatgtctaa gcctttgtct ttacagaaat
      541 caacgatttt ttgtggagct tcagccgcag taatgtctaa acctaaaaca gaacccccta
      601 aacgaccagc aacacgttgt aaatcggctt cttgagcagg aatgtccaag caaatcacgt
      661 gtgcgccatc acgagctaaa acttcagcaa tcgcttcacc aataccacgg ctagcacctg
      721 ttactaaggc tgttttgcca gctaaaggct ttttccaatc aatattaact tgtgtgtcgc
      781 tggcactgat gcgaacaact tgtgcagaaa cataagcaga ttttgctgat aagaagaaac
      841 gtaatgtcga ttcaacttgg ttttcagcat tgggggcaac ataaactaat tgtgctgtca
      901 ttgctttttt gaattcttta gcaacagcac gcacaaagcc ttctaatgca cgttgggcag
      961 tggcttgttt tggatcagta cagctttctg gcgtgcgacc taaaatcacc acacgaccac
     1021 agttttgcaa ttggcgtgcg ataggattaa agaaattata aacttcaatt aattgttcgc
     1081 tgttttttac cccagaagca tcaaaaacaa aggctttaaa tttaaattct ttatcttcaa
     1141 cattgtggct acgtaaagat aaacctgctt cagaggcagc agtttggtga ataaaatctt
     1201 ggttgcctgc ataagcttcg gcatgaatgt tttttaacac acgagcaata ggggcaacta
     1261 attcagcatt aggggctgca cctaataata cggcaccact gactaaaggt ttgttcgcat
     1321 cataacgttc caaaaccaaa ggtgcaggca agcctaagtt acgggttaat aaacggccaa
     1381 cgggcgaatt tgcaaataac tgataacgat cactcatggt ggtttccttt ggttgtcggg
     1441 ttatgagagg attgtcattt aggcacaaaa gtcaatcata accagtgtgg atggattcag
     1501 gccgagagac tagaacaaaa agcaccattt ggcgagactt cttccgccaa aaaaactaaa
     1561 atctcgttcg gctttttata acttatgtgg ttcttgccga tttgatgcgg atggcaaaca
     1621 atttaacgtt gctatgtgca aaatatacgg ttatagaaca acacctaagt tttactcttc
     1681 ataaagacta acaaaatgta tttctgagta cattcgcttt gtggcagact acgccacgcg
     1741 ctaaagccaa tgtaaattgt tgagtttatg ttagtttgac a
//
LOCUS       DAOQMB010000480         1777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_48439_length_1777_cov_15.876887, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000480 DAOQMB010000000
VERSION     DAOQMB010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48439_length_1777_cov_15.876887"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..1342
                     /locus_tag="PLY05_11275"
     CDS             134..1342
                     /locus_tag="PLY05_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HNC03920.1"
                     /translation="MLFAPEITPNAYTPPQRRLLGPGPSDISTRVLQALAKPTIGYLD
                     PSFVSMMEELKSLLRYAFQTKNMLTFPVSGPGSAGMEMCFVNLVSPNDKVIVCRNGVF
                     GGRMVENVERCGGIPIIVDDEWGKAIDVNKVADALKANPDTKVLAFVHAETSTGALSD
                     AAALCKLAQEYGCLTIVDTVTSLAGVPVLVDEWGADAVYSGSQKCLSCTPGLSPITFS
                     EKAIAAVKARQHKCQSWFLDLNLLLGYWGETTRTYHHTAPTNSLYALHEALLVLQQEG
                     LENAWARHQRNHLALKAGLSQLDIDYVVAEKDRLPQLNSIHIPTGLDDKQVRAALLNQ
                     HGIEIGAGLGALAGKIWRIGLMGYSSRVENVISCLSALESVLRQQGFNCPEGTAVAAA
                     YHCYAEQAQV"
     gene            1413..>1777
                     /locus_tag="PLY05_11280"
     CDS             1413..>1777
                     /locus_tag="PLY05_11280"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC03921.1"
                     /translation="MFRLMSMLILSVLMTACASPSRTVDSKPLTANPPVKARTMSISA
                     LNATASSQIKVQGTVPDSFDNSYWQFVQIDGQRMAKDDLAPVARFKVNELSGFTGCNS
                     FVAAYKRDGLWLTINNLRMG"
BASE COUNT          493 a          318 c          416 g          550 t
ORIGIN      
        1 cgttttagtg cgtggttggt acaaaaattc tcgccaaatt gattgcatct ttagtgtcgc
       61 ctcgctaggc taaggtctct taaaaatata acaatcctaa taaaataaag gataaaaaga
      121 ggttaacaat aacatgctat ttgcgccaga aatcacccct aatgcttata ctccacccca
      181 acgtcgttta ttagggccag gcccttcgga tatttccaca cgcgttttac aagccttggc
      241 taaaccaacg attggttatt tagaccctag ctttgttagc atgatggaag aattaaagtc
      301 tttattacgt tatgcctttc aaaccaaaaa tatgctgact tttcctgtgt cagggccagg
      361 ttcggcaggc atggaaatgt gctttgttaa tttggttagc cctaatgata aagtgattgt
      421 ctgtcgtaat ggtgtgtttg gtggccgtat ggtcgaaaac gtggagcgtt gtggcggcat
      481 tcccattatt gtcgatgatg aatggggcaa agcgattgat gtaaataaag tggccgatgc
      541 ccttaaagct aacccagata ccaaagtatt ggcttttgtt catgctgaaa cctcaacagg
      601 tgcgttatct gatgccgctg ctttatgtaa attggcacaa gaatatggtt gtttaacgat
      661 tgttgatacc gtgacttcct tagcgggtgt acctgtatta gttgatgaat ggggcgcaga
      721 tgctgtttat tctggtagtc aaaaatgttt atcgtgtaca ccaggtttat cgccaattac
      781 ctttagcgag aaggcgattg cggctgttaa agcgcgtcaa cataaatgcc aaagttggtt
      841 tttggattta aatttattat tgggttattg gggcgaaacc acacgtacct atcatcacac
      901 cgcaccaacc aatagtctgt atgctttaca tgaagcttta ttagtgttgc aacaagaagg
      961 tctagaaaat gcgtgggcac gccatcagcg caatcattta gccctaaaag ctggtttaag
     1021 ccaattggat atagattacg tggttgcaga aaaagatcgt ttgccacaat taaattctat
     1081 tcatattcca acaggtttgg atgacaaaca agtgcgtgca gctttactga atcagcatgg
     1141 tatcgaaatt ggtgcaggtt taggcgcatt agcaggtaaa atttggcgta ttggtttaat
     1201 gggctattcg agtcgtgttg aaaatgtgat tagctgtcta tctgctttgg agtctgtgtt
     1261 gcgtcaacaa ggctttaatt gtccagaagg tacagcagta gcagccgcat atcattgtta
     1321 tgctgaacaa gcccaagtgt agtttaaaga tattcctccc ctcatgtagg ggagtgttag
     1381 ggtggggtcg tatgctaaac aggagaaaaa agttgtttcg tttaatgtct atgttgattt
     1441 taagtgtgtt gatgacggct tgtgctagtc catcaagaac ggttgattca aaaccattaa
     1501 cggcgaatcc tcctgttaaa gcacgtacga tgagtattag cgcattaaat gcaacggcaa
     1561 gctctcaaat taaggtacaa ggcactgtgc ctgatagctt tgacaattct tattggcagt
     1621 ttgtgcaaat tgacggtcaa cgaatggcca aagacgattt agctcctgtt gcccgtttta
     1681 aagtcaatga gttatcagga tttacaggtt gtaatagctt tgttgctgct tataaacgtg
     1741 atggtttgtg gttaacgatt aataatttaa gaatggg
//
LOCUS       DAOQMB010000481         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49029_length_1766_cov_16.965517, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000481 DAOQMB010000000
VERSION     DAOQMB010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49029_length_1766_cov_16.965517"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1766
                     /gene="infB"
                     /locus_tag="PLY05_11285"
     CDS             <1..>1766
                     /gene="infB"
                     /locus_tag="PLY05_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNC03922.1"
                     /translation="AARKAAEEAARKAAEEDTLKGNEEAGSDQAKKATVANNNAKDKK
                     LSVPKPSVVLKSEKKEETPEEKAKREAEQAKQKAAEEARRKQEEAARKAAEEKARQRT
                     LEQARRIAAELEERQDDKGSSEDVPLARGLVGRAYEDSFAREDREIRRGGNTTGRGGK
                     SKVGKKREEEQAFRPNQKALSSALSKQHGFEKPVEKQVYEVAIGDSIVVADLAQKMAV
                     KAKEVIKVLMKMGEVVTQNQAIDQATAALVVEEMGHTAKYVSETAVEDSLIDDAQHDG
                     EASTRPPVVTIMGHVDHGKTSLLDYIRRAKVAAGEAGGITQHIGAYHVTTPRGVITFL
                     DTPGHAAFTAMRARGAKATDIVILVVAADDGVMPQTAEAIDHARAAGVPLIVAINKMD
                     KPSADPDRVLNELTVKQVVPEEWGGDTQIVKVSAHTGQGIDELLEAILIQSELLELTA
                     VEEGAAQGVVIEARVDKGRGAVASLLVQKGTLRAGDLVLAGQFYGRVRAMSDERGLPT
                     KSAGPSIPVEILGLPEAPNAGDEFIVVGDERKAREVAEFRANKERQSRLARQQGMKLE
                     NLFASMGKQEAPTVNIVLKTDVR"
BASE COUNT          541 a          329 c          462 g          434 t
ORIGIN      
        1 gcggcacgta aggctgctga agaggcagca cgtaaagcgg ccgaagaaga tacacttaag
       61 ggtaatgaag aagcaggtag tgaccaagct aaaaaagcaa cagttgcaaa taataatgct
      121 aaagacaaaa aattgtctgt gcctaaacca agtgttgtct taaagtctga aaagaaagaa
      181 gaaactcctg aagaaaaagc taagcgtgag gctgaacaag ccaaacaaaa agcggcagaa
      241 gaagcacgtc gtaagcaaga ggaagcggca cgtaaagctg ctgaagaaaa agctcgtcaa
      301 cgtaccttgg aacaagctcg ccgtattgca gcagaattag aagagcgtca agatgataaa
      361 ggttcttcgg aagatgtgcc tttagctcgt ggtttggttg gtcgtgctta cgaagattct
      421 tttgcgcgtg aagaccgtga aattcgtcgt ggtggtaata cgacaggtcg tggtggcaag
      481 tctaaagttg gtaaaaagcg cgaagaagaa caggctttcc gtccaaatca aaaagcatta
      541 agttcagcac tctccaagca gcatggtttt gaaaaacctg ttgaaaaaca agtttatgaa
      601 gtggctattg gtgatagcat tgttgttgct gatttggcgc aaaaaatggc tgtaaaagcc
      661 aaagaagtga ttaaagtctt gatgaaaatg ggtgaagtag ttactcaaaa tcaagccatt
      721 gatcaggcaa cagccgcttt agtcgttgaa gaaatggggc atacagccaa atacgtcagt
      781 gagaccgctg ttgaagactc cttgattgat gatgcacaac atgatggtga ggcaagtact
      841 cgtccgcctg ttgtcacgat catgggtcac gttgaccacg gtaaaacctc cttattagat
      901 tatattcgtc gtgccaaagt ggccgcgggt gaagcaggtg gtattactca acacattggt
      961 gcttatcatg tgaccacacc acgtggtgtg attaccttct tagatacacc aggccacgca
     1021 gcatttactg cgatgcgtgc tcgtggtgct aaggctacag atattgttat tttggtggtt
     1081 gctgctgatg atggtgtaat gccacaaacc gcagaagcca ttgaccatgc gcgtgctgct
     1141 ggtgtgccat taattgttgc aatcaacaaa atggataaac catctgctga tcctgatcgt
     1201 gtgttaaatg agttaactgt taaacaagtc gtgcctgaag agtggggcgg tgatacacaa
     1261 atcgttaaag tatctgccca tacaggtcaa ggtattgatg aattgttaga agccatctta
     1321 attcaatcag aattgttaga gttaacagcg gttgaagaag gtgcagcaca aggtgtggta
     1381 atcgaagcgc gtgttgacaa aggtcgtggt gcggttgcta gcttattagt acaaaaagga
     1441 actttacgcg caggtgactt agtattggcc ggtcaattct atggccgtgt acgtgcgatg
     1501 agtgatgagc gtggtttacc aaccaaatca gcaggccctt ctattcctgt tgagatttta
     1561 ggtttacctg aagcaccaaa cgctggcgat gaatttattg tggttggtga cgagcgtaaa
     1621 gcccgtgaag tggctgagtt ccgtgccaat aaagagcgtc aaagccgctt agcacgtcaa
     1681 caaggtatga agttagaaaa cttatttgct agcatgggca aacaagaagc accaacagtg
     1741 aatattgttc taaaaactga cgtacg
//
LOCUS       DAOQMB010000482         1763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49171_length_1763_cov_19.251756, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000482 DAOQMB010000000
VERSION     DAOQMB010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49171_length_1763_cov_19.251756"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..155)
                     /locus_tag="PLY05_11290"
     CDS             complement(<1..155)
                     /locus_tag="PLY05_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNC03923.1"
                     /translation="MSRRRVVVTGLGMVTSLGADVASTWSHILQGKSGISHIDHFDAS
                     QYKTRFAG"
     gene            complement(376..612)
                     /gene="acpP"
                     /locus_tag="PLY05_11295"
     CDS             complement(376..612)
                     /gene="acpP"
                     /locus_tag="PLY05_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_251656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNC03924.1"
                     /translation="MSNIEERVKKIVAEQLGVKLEDVKNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKINTVQAAIDYVVANTK"
     gene            complement(744..1478)
                     /gene="fabG"
                     /locus_tag="PLY05_11300"
     CDS             complement(744..1478)
                     /gene="fabG"
                     /locus_tag="PLY05_11300"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HNC03925.1"
                     /translation="MSLQGKVAIVTGASRGIGKAIAQQLASQGAIVVGTATTEAGAEA
                     ISQYLAEYNGAGLALDVTQADAIDNFLAQVQQRFGTPLILVNNAGITKDGLLLRMKDN
                     DWDTVINTNLTAIFRLSKACLKGMGKARWGRIINISSVVGQMGNAGQCNYAAAKAGLE
                     GFSRALAKEMGSRAITVNCIAPGFIKTDMTDVLTEEQQAVMLAQIPANRLGQPEEIAY
                     AVAFLASDQAGYINGVTLDVNGGMYM"
     gene            complement(1488..>1763)
                     /locus_tag="PLY05_11305"
     CDS             complement(1488..>1763)
                     /locus_tag="PLY05_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="[acyl-carrier-protein] S-malonyltransferase"
                     /protein_id="HNC03926.1"
                     /translation="DFHQAAIPVVQNVGATVEPNVTAIRQALVAQLYSPVRWVQTVEY
                     LAAQGVTRVVECGSGKVLCGLNKRINKALICESLENVAGLTAALAIK"
BASE COUNT          508 a          405 c          315 g          535 t
ORIGIN      
        1 ccagcaaaac gggttttata ttgactggca tcaaaatgat caatatgaga aattccactc
       61 tttccttgta aaatatggct ccatgtgctg gcaacatctg cccctaaact ggtaaccatg
      121 cccaaaccag tcacaacaac ccgacggcgg ctcatatgtt tctccaatta ttaacaacat
      181 ttaaaaacga aaactaggtg ttgtcgcgta tggtgagcgg agtcgaacca tagcaaataa
      241 cacttcgact tcgctcagtg tacgattaaa ttatatttta gtgcttattt aagcatattt
      301 atttgacatt aaaattgcaa aaaaaaaccg catgacccgc ggtaacggga tgcggtattt
      361 ttgtcagttg tccaattatt ttgtattggc aacaacgtaa tcaatggcag cttgtactgt
      421 attgattttt tcagcttctt catcaggaat ttcggtttca aattcttctt ctaaagccat
      481 gactaattca acggtatcta aagaatcagc accgagatca tcaacaaaag aagattcgtt
      541 tttaacgtct tctaatttaa cacctaattg ctcggcaacg atttttttaa cgcgttcttc
      601 gatgttactc actttctttg ttctcctgtg ggtagattct atttttattg atgttctgta
      661 tgagatcatc aataaacaat cgccgtagtg tatgaagctc tgcgctttat cgcaacacct
      721 aaacgattgt ttgattaaaa gagttacata tacataccgc cattgacgtc taaagtcacg
      781 ccattgatgt atcctgcttg gtcactggcc aaaaaagcaa cagcataggc aatttcttcg
      841 ggttgtccca aacggtttgc tggaatttga gccaacatca ctgcttgttg ttcttcggtt
      901 aaaacatcgg tcatatcggt tttaataaaa cccggtgcaa tacaatttac cgtaatcgcg
      961 cggctgccca tttctttggc taaggcgcgg ctaaaacctt ctaagcctgc ttttgcggcg
     1021 gcatagttac attgtcctgc attacccatt tggccgacca cagaactgat attaataata
     1081 cgtccccaac gtgccttacc catgcctttt aaacaggctt tagataaacg gaaaatggcc
     1141 gttaagttgg tattaatcac cgtatcccaa tcgttatctt tcatgcgtaa taataaacca
     1201 tctttggtaa tacctgcatt gttaaccaaa attaatggtg taccaaaacg ctgttgtact
     1261 tgggctaaaa aattatcaat tgcatcagct tgtgtgacat ctaatgccaa gccagcacca
     1321 ttatattctg ctaaatattg gctaatggcc tctgcacctg cctcggtggt ggctgtacca
     1381 accacaatcg caccttgact cgctaattgt tgagcaattg ccttaccaat accacgactt
     1441 gcgcctgtca caatggcaac tttgccttgt aatgacataa taaactccta tttgatcgcc
     1501 aaagctgccg ttaatcctgc aacattttct aaagactcac agatcagtgc tttgttaata
     1561 cgtttattta aaccacacaa cactttgccc gaaccacatt caacgacacg agtgacacct
     1621 tgtgcggcta aatactcaac ggtttgtacc caacgtacag ggctataaag ctgtgctact
     1681 aaagcttggc gaatagcagt gacatttggt tcaaccgttg ccccaacatt ttgcaccaca
     1741 ggaattgcag cttgatgaaa atc
//
LOCUS       DAOQMB010000483         1753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49700_length_1753_cov_5.216137, whole genome shotgun sequence.
ACCESSION   DAOQMB010000483 DAOQMB010000000
VERSION     DAOQMB010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49700_length_1753_cov_5.216137"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(82..204)
                     /locus_tag="PLY05_11310"
     CDS             complement(82..204)
                     /locus_tag="PLY05_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC03927.1"
                     /translation="MNATKSTKKPTYSLEFKQDAAKLVIEKGYSCQKAADHLGV"
     gene            complement(346..1614)
                     /gene="cmr1"
                     /locus_tag="PLY05_11315"
     CDS             complement(346..1614)
                     /gene="cmr1"
                     /locus_tag="PLY05_11315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HNC03928.1"
                     /translation="MLRKPPTSLDPDKALASTQAPTWTTLECELVTPMYGGGVATHTV
                     DEKMPIRVSAIRGQLRFWWRLLATHLWRLGSVQAIRQAEADLWGGIDDKTKASKVFLK
                     VSDIRSLNITPWAKYNTNPRTGQYKSLPDPENWANVPYVLFPAQGKRPGGADAEAPHS
                     LAKAGLNFQLHLTFDNITDIQQKQVWEALRWWANFGGLGARTRRGLGAVKVSSLENQA
                     LIRPITIDEANKVGCQLITRQQVNSSYQAFTESVNRLQKFRQVDIGRPNHSSRSRWPE
                     PDAIRRIKNQYLVDGAKTHRPIHQAGNLFPRAAFGLPIIFKFKDDNIRGNGNQPEPSQ
                     TSLQPIVGDSVKERMASPLILRPYFDGNRWRAAALLLPCGHINNLKLDLSGDKATYWN
                     PAQAQNVPPIAQNGGIDALSAFMNFFAKKG"
BASE COUNT          489 a          362 c          337 g          565 t
ORIGIN      
        1 tcgtggactt tgttgctttc ataaacacct ctacttgcag tataaatact acctttcaaa
       61 gtgtcctgtt ttattaaacc attacacccc taaatggtct gctgcttttt ggcagctata
      121 gcctttctca atcactaatt tagccgcgtc ttgcttaaac tctaaactgt aggtcggctt
      181 tttcgtggac tttgttgcat tcataaacac ctctcgttgt agtataaata ctacctatca
      241 aagtgtcctg ctttattaaa ccattacacg actacgacgg gcagcggcaa taaaaccttg
      301 tacagggcca atagagataa taagtacata atctgtcatg ttttgctatc cttttttggc
      361 aaaaaagttc ataaaggcag agagggcatc tatgccgcca ttttgggcaa taggcgggac
      421 gttttgggct tgagcggggt tccaatatgt tgctttatca cctgataagt cgagctttaa
      481 gttattaata tggccacaag gtaataatag agcagcagct cgccaacgat tgccatcaaa
      541 ataaggtctt aaaattaaag gactagccat tcgttcttta actgaatcac caactatcgg
      601 ttgtaaagat gtttgagatg gttctggttg attaccattg ccacgtatat tatcgtcttt
      661 gaatttaaaa ataatcggta aaccaaaagc agcacgagga aataaattac ctgcttggtg
      721 tattggtcta tgggtttttg ccccatcaac taagtattgg tttttaattc gacgaatagc
      781 atcgggttct ggccagcggc ttctactaga atgatttgga cgaccaatat cgacttgtct
      841 aaatttttgt aaacgattaa cagattcggt gaaggcttga taggagctgt ttacttgttg
      901 tcgtgtaatg agttggcagc caactttgtt agcctcatca attgtaatag gtcggattaa
      961 tgcttgattt tctaaagaac ttacttttac agctcctaaa ccacgacgag tacgcgcacc
     1021 taaaccacca aaatttgccc accaacgcaa agcctcccaa acttgttttt gttgaatatc
     1081 ggtaatgttg tcaaaggtta aatgcaactg aaagtttaat cctgcttttg ctaatgagtg
     1141 aggtgcttcg gcatctgctc caccaggtct tttaccttgt gcaggaaata aaacataagg
     1201 aacatttgcc caattttctg gatcaggtag tgatttatat tgtccagttc taggattagt
     1261 attgtattta gcccatggag taatattaag acttcgaata tcgctaactt tcaaaaaaac
     1321 cttactcgct ttagttttat catcaatacc tccccacaag tcggcttctg cttgacgtat
     1381 ggcttgcaca ctacccaacc gccataaatg tgttgccagc aaacgccacc aaaaacgtaa
     1441 ctggcctcta atagcactca cacgaatagg cattttttca tcaacagtat gtgtagcaac
     1501 acctccacca tacattgggg tgactaactc acattctaag gttgtccatg tgggggcttg
     1561 agtgcttgct agtgctttat cagggtcgag tgaagtaggt ggttttctta acatagttaa
     1621 tccctcagat tagtttttga atttatcaac aatttcaatg gcatcgggtg agacatctaa
     1681 ataaaaagga tttccttttt tgtcttgttt aatcgcaatt cttgccgcta gctctaggcc
     1741 taatttctct tct
//
LOCUS       DAOQMB010000484         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49745_length_1752_cov_14.182675, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000484 DAOQMB010000000
VERSION     DAOQMB010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49745_length_1752_cov_14.182675"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            120..1285
                     /locus_tag="PLY05_11320"
     CDS             join(120..378,378..1285)
                     /locus_tag="PLY05_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612080.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC03929.1"
                     /translation="MSKKVQRQYSAELKASAVKMVIEELKTLSQVSQDLHIPLQTLHS
                     WVNKGRKGELSGVGTYNPDLVALQTEVRQLKKQLAAAEMERENLKKGGGVFCEGHPAK
                     YAFMRNYHKTYPLSVMARLLGVSVTGYFDWLHRKPSKRQREREQALKLVTAAHEATRE
                     SYSAVRLHQHLRNQGHSLSLHRVRSLREVNNIRCKRHKRFKNTTNSDHQKPVYDNHLK
                     QQFEVERPNVAWSCDITYVLTDEGWLYVAGVKDVYTKELVGYCLSSRMTADIVVQALT
                     MAIKRRKPMKGLIVHSDRGSQYCSHIYRDLITQHQFVGSMSAKGNCYDNAPIESFWAT
                     LKNELVYHQHYATRMEAIKDIQYFIEIFYNRMRIQAKLNFRSPAQAFEQFKRAA"
BASE COUNT          568 a          332 c          374 g          478 t
ORIGIN      
        1 gacgtgaggc aacatggaat gccaaaatgg tcgatcgtta tcctgatttg attatgatgt
       61 gtgttgaaaa aaccgtatga ctagatttgg taaacttaag aagattattg gagtttatca
      121 tgtcaaagaa agtacaacgg caatacagtg cagagttaaa ggcaagtgcg gttaaaatgg
      181 tgattgaaga gttaaagacg ttaagccaag tctcacaaga cttacatatc ccgcttcaaa
      241 cactgcatag ttgggtcaac aaagggcgta aaggggaatt atcaggcgtt ggcacctaca
      301 atcctgattt agtggcctta cagacagaag tacggcaact gaagaaacaa ttagccgcag
      361 cggaaatgga gcgtgaaatc ttaaaaaagg cggcggcgta ttttgcgagg gacaccctgc
      421 caaatacgcg ttcatgagga attatcacaa gacttaccct ttgtcggtga tggcgcgatt
      481 attgggtgtc agtgtcacgg gttactttga ttggctacat cgtaagccct caaagcgaca
      541 acgtgagcga gagcaagctc tgaagttggt gacggcggca catgaagcga ctcgggagag
      601 ttatagtgct gtcaggctac atcagcactt gaggaatcaa gggcatagcc tctcgttgca
      661 tcgtgtacgc agcttacgtg aagtcaataa tattcgctgc aaacgccata aacgctttaa
      721 aaacaccacg aatagcgacc atcaaaagcc tgtgtatgac aatcatctca aacaacaatt
      781 tgaggtagaa cgtcccaatg tggcatggtc ttgtgacatt acttatgttt taactgatga
      841 aggttggtta tatgtggctg gagtaaaaga cgtgtatacg aaagaattgg tgggctattg
      901 cctatcttct cgcatgacgg ctgatattgt ggtgcaggca ttaacgatgg cgattaaacg
      961 gcgcaagccg atgaaagggt taattgtcca ttctgataga ggcagtcaat actgtagcca
     1021 tatttatcgt gatttaatca cacaacatca atttgtgggt tcaatgagtg ctaaggggaa
     1081 ttgttatgat aatgcgccca ttgaaagctt ttgggcaacg ttaaaaaacg agttggttta
     1141 ccatcagcac tatgcaacgc gtatggaagc catcaaggat attcaatatt ttattgagat
     1201 tttctacaat cgaatgcgaa ttcaagctaa actaaatttt aggtcgccag ctcaagcatt
     1261 tgaacagttt aagagggcag cttaatttat cccaagtcca gtcatacggt aaactcaaca
     1321 cacatcactt taattatttc taagtcatcg tgttcactgc tcatagaatc cctcacttag
     1381 cctaacttaa ttttacacac caaaacctct aatccactta tggcggatta taacgactta
     1441 tgtacaatat aactaacact taaaaacaag gactcaccta ctctttaagt cataaaaagt
     1501 gttgcaaaag atgactctac actatcaata cgattagatt aaatgtgaag ttaaattgtt
     1561 ggtagtgtgt aaaaccaata tgataattct atgtaacctt tacttaccaa cggctagaag
     1621 atttttatca cgtcaaattt atgcactacc aattttttta taagcaataa gtattaaata
     1681 ctttataggc aaaataggcc taactttaac gctaaggggt agcaacccca caacaaacta
     1741 aaacctgaga tt
//
LOCUS       DAOQMB010000485         1752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49746_length_1752_cov_14.057749, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000485 DAOQMB010000000
VERSION     DAOQMB010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49746_length_1752_cov_14.057749"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLY05_11325"
     CDS             complement(<1..413)
                     /locus_tag="PLY05_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HNC03930.1"
                     /translation="MKKSFVALVALLSVGSAFAASMPKGDATAGQALSAQCAACHGAD
                     GNSAAPTFPRLAGQNAKYIYKQLQDFKAGRRNNALMMPMAADKTDQQMADLAAYFSQQ
                     KSGVNLADPALVAKGERLYRGGNPATGVAPCAGCHG"
     gene            complement(515..808)
                     /locus_tag="PLY05_11330"
     CDS             complement(515..808)
                     /locus_tag="PLY05_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNC03931.1"
                     /translation="MKKLAPTVAFFSVLIVMPTYAADIETKYGQSCATCHGAGALGAP
                     KKGDKAAWEPRLKQSTETLLSHVRNGYKNMPAKGLCNDCSDDDYIALIKFMSQ"
     gene            1106..1687
                     /gene="yihA"
                     /locus_tag="PLY05_11335"
                     /pseudo
     CDS             1106..1687
                     /gene="yihA"
                     /locus_tag="PLY05_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
BASE COUNT          533 a          380 c          343 g          496 t
ORIGIN      
        1 ccatgacaac ccgcacaagg tgcaacacct gttgcaggat taccaccacg gtacaaacgt
       61 tcacctttag caactaaggc tgggtcggct aaattaacac ccgatttttg ctgagaaaaa
      121 taagctgcta aatcggccat ttgctggtct gttttgtcag cggccattgg catcattaag
      181 gcattgttac gacgacctgc tttaaaatct tgtaattgct tatagatgta tttagcattt
      241 tggcctgcca aacgtggaaa tgttggtgca gcactattac catcagcacc atgacaagca
      301 gcacattgcg ctgataaggc ttggccagca gtagcatcac ccttaggcat actagctgca
      361 aaagcactac caacagacaa aagagcaact aaagcaacaa aagacttctt cataatagct
      421 tccaatagct tattgaggac tgacgtggtt atcgctgatt tgtaaatggc aaatttacaa
      481 aacgcgctaa ttgcagtgca atacgtcaac cctgttattg agacatgaat ttaatcaggg
      541 cgatgtagtc atcatcacta caatcgttac acaaaccttt ggctggcata tttttgtagc
      601 catttcgaac gtgggacagt aatgtctctg tgctttgttt taaacgtggc tcccaagccg
      661 ccttatcacc ttttttaggc gcgcctaaag ctcctgctcc atgacaggtt gcacacgact
      721 gaccatattt agtctctatg tcggccgcgt atgttggcat cactatcaaa acactaaaga
      781 aggccactgt tggggctagc ttcttcatga cgttctccca cggttctaac cgttgcgcga
      841 ttatatcgtt atctgtaaca aactgtaata cccttagaca atatgagcct tatagttggc
      901 attaaaaatt gtacaaaaat acaccaaaaa gacgattttt tacaaaattt ggcttagaag
      961 ccatttatga ttaattattg ttcattttgt agataaaagc aaaaaaccct catttgactt
     1021 ttttaggact tgattggacg ttgtataata gctgactttt tacttgtgat agacctttgt
     1081 tatgactcat cccatgctga ctttattgcg ccaagccaaa tttatgacca gtgcgcctcg
     1141 tttatcggct tgccctccca atattggcta tgaagtcgct tttgcagggc gttctaatgc
     1201 aggtaaatcc agtgcaatta atgccctcac caatcaacga caattagctc gtgcctcaaa
     1261 aacaccgggg cgaacacaat tactcaatta ttttagtttg gatgaagaac gtcgtttagt
     1321 tgacttacct ggttatggtt atgcggctgt gcctttagcg atgaaagaag catggcaaaa
     1381 agaactagaa aactatttga ttaagcgtga aagcttacaa ggtttaattg tgttaatgga
     1441 tgtgcgtcat ccactgcaaa aatacgatga aatgttatta aactgggcgc aacgtcgcca
     1501 actcaatgta catatcttat taactaaatc agataaatta aaccgtagcc ctgcccttgc
     1561 tgcactacaa gccatgaaaa aacaattaaa agacaagaat attgtggcga ctgtgcaatt
     1621 attttcagcc ttacaaaaaa caggtttaga agaagccgcc gctgtgctgg ggcaatggtt
     1681 aaattatcca ccacaagttg aaaatgaagc agctatcaca accgacagtc aaccatgatt
     1741 aacaaaacag gg
//
LOCUS       DAOQMB010000486         1750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_49861_length_1750_cov_17.336873, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000486 DAOQMB010000000
VERSION     DAOQMB010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49861_length_1750_cov_17.336873"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            151..1125
                     /gene="cysK"
                     /locus_tag="PLY05_11340"
     CDS             151..1125
                     /gene="cysK"
                     /locus_tag="PLY05_11340"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004749879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNC03932.1"
                     /translation="MAKIYDDNALSIGNTPLVRLNRVVEGHKKVYAKIEGRNPAYSVK
                     CRIGANMIWQAEKDGLLKAGMEIVEPTSGNTGIALAYVAAAKGYKLTLTMPASMSLER
                     RKVLKALGANLVLTEAAKGMKGAVDKAEEIRASNPEHYFLPQQFENPANPAIHEKTTG
                     PEIWQDTDGEVDIFIAGVGTGGTLTGVSRYIKQTQGKAIQTIAVEPEASPLISQSLAG
                     ENLQPAAHKIQGIGANFVPKNLDLTLVDGVERVSNDDAIAYAQRLMREEGILAGISCG
                     AAVAAAARVAARPENADKTIVVILPDSGERYLSTVLFDGLFTEQEMVQ"
     gene            1236..>1750
                     /gene="ppa"
                     /locus_tag="PLY05_11345"
     CDS             1236..>1750
                     /gene="ppa"
                     /locus_tag="PLY05_11345"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HNC03933.1"
                     /translation="MSYNNIPPGKDAPNDIYVVIEIPANHDPIKYEIDKDSDALFVDR
                     FMGTAMFYPANYGYIPNTLSEDGDPLDVLVVTPYPVAPGSVIRCRPVGKLNMEDESGI
                     DAKLIAVPHDKLTPIYKNVKEYTDLPELLINQIRHFFETYKALEPGKWVKVTGWEGAE
                     VAKAEVLKSIEA"
BASE COUNT          545 a          340 c          396 g          469 t
ORIGIN      
        1 agagccgcac ctaaaaaaat ggcggcttgg ccaagaagcg tcatgcttaa tgctccgttt
       61 gtatatcaat agatgaaaaa gattgttgca agtgtgacac aaactcgcca aagtatgtgt
      121 ttttacgagc ataaaaatta ggaaaatatt atggcaaaaa tttatgacga taatgcgtta
      181 tcgattggta ataccccatt agtccgttta aatcgcgtag tggaaggaca taaaaaagtc
      241 tatgccaaaa ttgaagggcg taatcctgcc tattcagtga agtgtcgtat tggggctaat
      301 atgatttggc aagccgaaaa agatggttta cttaaagcag gcatggaaat tgttgagcca
      361 acatcgggta acacaggcat tgctttggcc tatgttgccg ccgccaaagg ctacaaattg
      421 accttaacca tgccagcctc gatgagttta gaacgccgta aagtgttaaa agcacttggt
      481 gctaatttgg tgttaaccga agcagctaaa ggcatgaaag gcgcggttga taaagccgaa
      541 gaaattcgtg ccagcaatcc tgagcattac tttttaccac aacaatttga aaaccctgct
      601 aacccagcca ttcatgaaaa aaccactggc cctgaaattt ggcaagatac tgatggtgaa
      661 gtggatattt ttattgcggg ggtgggtact ggcggcacat taacgggtgt gtcacgttat
      721 attaaacaaa cgcaaggcaa ggcgattcaa accattgccg ttgagccaga agcttcacca
      781 ttgattagcc aaagtttagc aggtgaaaat ttacaacctg ccgcgcataa aattcagggt
      841 attggcgcaa attttgtgcc taaaaatttg gatttaacgc tggttgatgg cgtggaaaga
      901 gtaagcaacg atgatgcgat tgcttatgca cagcgtttaa tgcgcgaaga aggtattttg
      961 gctggaattt cttgtggtgc agccgtagcc gcagcagcaa gagtggcagc acgtcctgaa
     1021 aatgccgata aaaccattgt ggtaatttta ccagactctg gtgagcgtta tttatcaaca
     1081 gttttgtttg atggcttatt cactgaacaa gaaatggtgc agtaaacaga gtgtttttat
     1141 ctgctgccag ttggcaagtt ttatatcaag ggtataatac gccacgattt ccacgctggc
     1201 caatctctag gccagtttat caaagagagt aaaccatgag ttacaacaat atcccacctg
     1261 gaaaagacgc accaaacgac atttatgttg tgatcgaaat tcctgctaac catgacccaa
     1321 tcaaatacga aatcgacaaa gacagcgatg ctttatttgt tgaccgtttc atgggtacag
     1381 ccatgtttta cccagctaac tatggttata tcccaaacac cttatctgaa gatggtgacc
     1441 cattagacgt attagttgta actccatacc cagttgcacc tggttctgtg attcgttgcc
     1501 gtccagttgg caagttaaac atggaagatg agtctggtat tgatgctaag ttaattgctg
     1561 taccacacga caaattaaca ccaatctaca aaaacgttaa agaatatacc gatttgccag
     1621 agttgttaat caaccaaatc cgtcatttct tcgaaactta caaagcttta gagccaggta
     1681 agtgggttaa agtaacaggt tgggaaggtg ctgaagtcgc taaggctgaa gtattaaaat
     1741 ctatcgaagc
//
LOCUS       DAOQMB010000487         1747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_50036_length_1747_cov_4.980496, whole genome shotgun sequence.
ACCESSION   DAOQMB010000487 DAOQMB010000000
VERSION     DAOQMB010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50036_length_1747_cov_4.980496"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(413..667)
                     /locus_tag="PLY05_11350"
     CDS             complement(413..667)
                     /locus_tag="PLY05_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03934.1"
                     /translation="MTIPQAVNLGLTGDKISIAITGTSDVSAARTAIATGAGTALGAA
                     ASGALVVAASAAGFATAPITVPLAAAAGFVSYVSSRFKWW"
     gene            888..1319
                     /locus_tag="PLY05_11355"
     CDS             888..1319
                     /locus_tag="PLY05_11355"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC03935.1"
                     /translation="MKEPVAMTTYPAVVGAILIARRKELGLSQNALAESVGLTVSTWS
                     RIENGDSALTIEQLKLAGDKLNVLPSQILKEADEKIIELKNRGIETNPVRVSINEIHA
                     SGIIPLVGTALFGLISTPLVLAAASVTASAVVTKLAKKFED"
     gene            complement(1373..>1747)
                     /locus_tag="PLY05_11360"
     CDS             complement(1373..>1747)
                     /locus_tag="PLY05_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03936.1"
                     /translation="KRRAIVKVLGSMDAYTLEVPKDLLDVLSEDEKAEVESFIADTKA
                     KNKKQSDTFSVQYIASNLDRVADLVLDGVEDYGLSEQWGGEVWAALEKMQKALKKKGY
                     TRPKREAKPKKTEDTQAELKLG"
BASE COUNT          492 a          362 c          336 g          557 t
ORIGIN      
        1 atggttatat cgttgccgca ctccgcagcg ttattagcgg atttgttgac ggtggatttg
       61 attatctgct gtttaacctg ttttcaaaca aactcgttgt tactttgatg ctttttagcc
      121 aatttaaccg atttgttttt gaaacaaggt ctttttcatc tgacgtgtga taaccgttag
      181 atgtaacttg gttaatttga ttattatcca agttccaaac cccagcattt gctggggttt
      241 atcttttttt gaggttggtt agaggtggcg ttttgtgccg cttttaagtt cgctatcgtc
      301 gcgcctcgat gggttgcttt tttggtcagg ttttgggtta atcgcgctac tgaaaaaatt
      361 ggtttaacac agcaaataat aatcaaatca ggtatctaac ctcatgttag cgttaccacc
      421 atttgaaacg actagataca tacgaaacaa aacctgccgc agcagctaat ggcacagtaa
      481 taggtgccgt tgcaaaacca gctgcactag ccgcaacaac taaagcacca ctagcagcag
      541 caccaagtgc agtacctgca ccagttgcaa tagcggttct tgctgcacta acgtcagaag
      601 tacccgtaat cgctatagaa atcttatctc cagtaagacc taaattaaca gcttgaggga
      661 tagtcatatt ttttctccga aagataaata ataattcaaa tgtaaaggtt ttattaccta
      721 acactgaacg gcacacctgt ttaattaaag ccatgccgtg aaatgaatat agtcgatcaa
      781 ctttaatttt tcaatgggaa tttgcatgaa ttgcaatttt ttttataaag caattttcaa
      841 tataatgcat atctttatgc tataatcgaa cggaggagat taattttatg aaagaacctg
      901 ttgcaatgac gacctaccct gctgtagtag gagcaattct gattgcaaga cgaaaagaac
      961 ttggcttatc acaaaatgca ttagctgaat ctgttggtct tacagtctca acatggtcac
     1021 gtattgagaa tggtgactcc gcattaacta ttgaacagct gaagcttgca ggagataagc
     1081 tcaatgtatt accaagtcaa atactgaaag aagctgatga aaaaataata gaactaaaaa
     1141 atagagggat tgaaacaaat cccgtcagag taagcattaa tgaaattcac gcctctggga
     1201 ttattccatt agttggcaca gcgttatttg gattaattag cactccctta gtgttagctg
     1261 ctgcaagtgt caccgcaagt gcggttgtaa ctaaattagc taaaaagttt gaggactaat
     1321 agaaaccctt tagattatct gtgtgacgaa cgatgtaact gttttttatt tttcacccaa
     1381 gctttagctc tgcttgtgtg tcttccgttt ttttcggctt tgcctctctc ttgggtcggg
     1441 tatatccttt cttcttgagt gccttttgca ttttttctag ggctgcccac acctcacccc
     1501 cccattgctc actcaatccg taatcctcaa caccatccag caccaaatcg gcaactctgt
     1561 ctaagttaga ggcgatatat tgaacactaa acgtgtcact ctgttttttg ttcttagctt
     1621 tggtatcggc tatgaaagac tccacttcgg ctttttcgtc ctcactcagt acatcgagca
     1681 agtctttggg tacttctaaa gtgtacgcat ccattgaccc taatacctta acgatagcac
     1741 gccgctt
//
LOCUS       DAOQMB010000488         1738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_50535_length_1738_cov_17.084373, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000488 DAOQMB010000000
VERSION     DAOQMB010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50535_length_1738_cov_17.084373"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1738
                     /locus_tag="PLY05_11365"
     CDS             <1..>1738
                     /locus_tag="PLY05_11365"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03937.1"
                     /translation="INASMGMLISLFIAFSITPWLALKLMAKHTPTSSHDSHEVHSPL
                     EPVFRKIMTPFLHPQQGQKRRHGLYLSIVVLIALAISLAVVQWVVLKMLPFDNKSEFQ
                     ILVDMPTGTPVEKTAAVLHDLGAYLATVPEVTDYQAYAGSSAPINFNGLVRQYYLRAQ
                     AEQGDIQVNLVDKHHRHQQSHAIVSRIRADLQNIARQHQAKLKVVEVPPGPPVLAPIV
                     AEIYAPDQQSQQQVATLLEAKFKQQAGMVDIDSSVVAQAMQKRLLVDQNKAMMLGVNS
                     QTITQTLQLALTGQQSSVLFANDAKYAVPMRVSLADNDKSQLANILKLQVKADDGSLI
                     PLSELVNISEEPSEQAIYHKDLLPVSYVFADFAGKLDSPLYGLFAMRDGLAQLNLPYS
                     AKLTEYFIRQPDDALREFAIKWDGEWQITYETFRDMGIAYAVGLVLIYLLVVAQFHSY
                     AIPAVIMMPIPLTIIGVMPSHALLGAQFTATSMIGMIALAGIIVRNSILLVDFIQLKL
                     AEGMALDEAVIQAASTRAKPIMLTGLAAMLGALFILDDPIFNGLAISLISGILVSTFL
                     TLVLIPLLYFGL"
BASE COUNT          453 a          369 c          385 g          531 t
ORIGIN      
        1 cgattaatgc ctcgatgggc atgctcattt ctttgtttat tgcctttagc attaccccgt
       61 ggttggcttt aaaattaatg gccaaacata cccccaccag tagtcatgac agccacgaag
      121 tccactcccc tttagagcca gtctttagaa aaatcatgac accttttttg cacccacaac
      181 aaggccaaaa acgtcgtcat ggtttgtatt tgagcattgt agtattaatt gccttagcca
      241 tcagtttggc agtggtgcaa tgggtggtgt taaagatgtt gccctttgat aataagtctg
      301 aatttcaaat tttggttgat atgccgacag gcacacctgt agaaaaaacc gccgccgtat
      361 tacacgattt aggcgcgtat ttagccaccg ttcccgaagt cactgattat caagcctatg
      421 caggtagcag cgccccaatt aactttaatg gtttggtgcg tcagtattat ttacgcgctc
      481 aagccgaaca aggcgatatt caggttaatt tagtggataa acatcatcgc caccaacaaa
      541 gccatgccat tgttagtcgt attcgtgccg atttgcaaaa cattgctcgt caacatcaag
      601 cgaaactaaa agtggttgaa gtgccacccg gcccgcctgt gttagccccg attgttgctg
      661 aaatttatgc accagaccaa caaagccaac aacaagtggc caccttgtta gaagccaaat
      721 ttaagcaaca ggctggcatg gtcgatattg atagtagtgt cgtcgcccaa gccatgcaaa
      781 aaaggctatt ggttgaccaa aacaaagcca tgatgttggg cgttaactcg caaaccatta
      841 cccaaacgct acaactggcc ttaacaggtc aacaaagcag cgtattgttt gccaacgatg
      901 ccaaatatgc tgtgccgatg cgtgtcagtt tggcagataa cgacaaatca caattggcga
      961 atattttaaa attacaggtt aaagcggatg atggctcgtt aattcctttg tctgaattgg
     1021 tcaacattag cgaagagcca tctgaacaag cgatttatca taaagattta ttgccagtta
     1081 gttatgtgtt tgccgatttt gcaggaaaac tggattcgcc gttatatggt ttgtttgcca
     1141 tgcgtgacgg tttagcgcaa ttaaacttgc cctactcggc caaactgacg gagtatttta
     1201 ttcgtcagcc tgatgatgcc ttgcgtgagt ttgccattaa atgggatggt gaatggcaaa
     1261 ttacctatga aacctttaga gatatgggca ttgcctatgc ggtgggtttg gtgttgattt
     1321 atttgttagt cgttgctcaa tttcactctt atgccattcc tgcggtgatt atgatgccaa
     1381 ttcccttaac gattattggc gttatgccca gccatgcttt gttgggcgca caattcaccg
     1441 ccacctcgat gattggcatg attgccttgg caggtattat tgtccgtaat tcgattttgt
     1501 tggtagattt tattcaatta aaattagccg aaggcatggc cttggatgaa gcagtgattc
     1561 aagctgccag cacacgtgct aaaccgatta tgctcacagg tttagccgct atgttagggg
     1621 cgttatttat tttggatgac ccgattttta atggtttagc catttcctta atttcgggga
     1681 ttttggtgtc aacgttttta actttggtgt taattccctt gttgtatttt ggcttgca
//
LOCUS       DAOQMB010000489         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_50670_length_1736_cov_3.607377, whole genome shotgun sequence.
ACCESSION   DAOQMB010000489 DAOQMB010000000
VERSION     DAOQMB010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50670_length_1736_cov_3.607377"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PLY05_11370"
     CDS             complement(<1..1090)
                     /locus_tag="PLY05_11370"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HNC03938.1"
                     /translation="MISKNRQRKKSVPDCQKPVVAPTVATEPTKHEPTTIRVLAETNQ
                     PVMIEVENALPVADSQPVKEDSVPTVASDAPNTQIIPEHFSQWCVPNGQWQVLAEAVV
                     GLSHRDKNLPCQDAVACQAAPRVCLVVCDGAGSSVVSELGANALAQGMSLLCHSLEAF
                     WVDLLDSPTTHDALLEKMTRLVLRHAKGIITQLATQHKREARDFRSTLLMLVVGKAHL
                     FWLKVGDGALVIEQIEHRFSQPALPSDGLVPVLKTLGELGKGEFANQTTFVDDALKFS
                     DVQYGLYCSQYLTGFAAMSDGAAEKLVSLNQQQVAGRISQWFDALRQQRLSRKTLTQA
                     FYGDDFNKNSTGDDRSIALAACGYNLTNL"
     gene            complement(1149..>1736)
                     /locus_tag="PLY05_11375"
     CDS             complement(1149..>1736)
                     /locus_tag="PLY05_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03939.1"
                     /translation="QSLHRQQIMIGDYNLNNFLCDPKSKTISFIDCDSYQCRLNQQFF
                     ACPVGSPDLTPPEHQNKPFKEVVRNPQSEVFSLAIILFKCLMLGRHPYDMVNGEDPVS
                     NMNSGRFAYGKGNQGIPKGDWYNIWSHMPYQLKSLFIRTFSEGALNPSLRPSLDDWHQ
                     ALSLYVKEMDKGWHEVAVRPEHAKKSEYRGTNSVS"
BASE COUNT          477 a          422 c          367 g          470 t
ORIGIN      
        1 acaaattcgt taagttatag ccacaggctg ccaaggcaat actgcggtca tcacctgtgc
       61 tgtttttgtt aaagtcgtca ccataaaaag cctgcgttaa ggttttgcga gacaaacgct
      121 gttggcgcaa ggcatcaaac cattggctga ttcggcctgc aacttgttgt tgatttaacg
      181 acaccagttt ttcggcggct ccatcactca tcgcggcaaa gcctgtcagg tattggctac
      241 aatacaaacc atactgcaca tccgaaaact taagtgcgtc atccacaaag gtggtttgat
      301 tggcaaattc acctttaccc aattcgccta aggtttttaa tacaggcacc aaaccatcac
      361 tcggcaaggc aggttggcta aaacgatgct caatttgctc aatcaccaat gcgccatcac
      421 ctacctttag ccaaaacaag tgcgccttgc ccaccaccaa catcaacaag gtcgaacgaa
      481 aatcacgggc ttcgcgtttg tgctgtgtcg ccaactgagt aataatccct ttggcatggc
      541 gcaataccaa acgggtcatt ttttctaata aagcatcgtg agtggtaggg ctatctagca
      601 agtccaccca aaatgcttct aaactatggc ataacaaact catgccttgt gccaaagcat
      661 ttgcgcccaa ttcagatacc accgaactgc ctgcaccatc gcacaccacc aaacaaacac
      721 gcggagccgc ctgacaagcc accgcatctt ggcaaggcaa gtttttgtca cgatggctta
      781 agccaacaac ggcctcggct aatacttgcc attggccatt aggcacacac cactggctaa
      841 aatgctcggg gataatttgg gtgtttggtg catcgcttgc tacggttggt acagagtctt
      901 cttttacagg ctggctatct gccacaggca acgcattttc aacttcaatc atcacaggct
      961 gattggtttc ggccaacacc ctgatggttg ttggttcatg cttagttggc tcagttgcaa
     1021 ctgtaggtgc aacaacaggc ttttgacaat ctggtactga ttttttgcgt tgacgatttt
     1081 tgctaatcat aattttgctc caataactta aaaataactg acagactgtg taaaaacgcc
     1141 acacggtact aactcaccga atttgtgcca cgatattctg attttttggc gtgttcgggt
     1201 ctaaccgcca cttcgtgcca gcctttgtcc atttctttta catacaaaga caaagcttga
     1261 tgccagtcat ccaaactggg tctaagcgaa ggattaagcg caccttcgct aaaggtacga
     1321 ataaatagac ttttaagctg gtagggcatg tggctccaaa tgttgtacca atcgcctttg
     1381 ggaatgcctt gattgccttt gccataagca aaacggccag agttcatatt gctcactggg
     1441 tcttcgccat tgaccatgtc ataagggtga cgacctaaca tcaggcattt aaacaaaata
     1501 atcgccaatg aaaacacttc ggattgcgga ttacgtacta cctctttaaa gggtttattt
     1561 tgatgttcgg gtggggtaag gtcggggcta ccgacagggc aagcaaaaaa ctgctgatta
     1621 agccgacatt gataactgtc gcaatcaata aaactaatcg ttttgctttt tgggtcgcac
     1681 aaaaagttat ttagattata gtcaccaatc ataatttgtt gacgatgcag gctttg
//
LOCUS       DAOQMB010000490         1735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_50710_length_1735_cov_16.807738, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000490 DAOQMB010000000
VERSION     DAOQMB010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50710_length_1735_cov_16.807738"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1735)
                     /locus_tag="PLY05_11380"
     CDS             complement(<1..>1735)
                     /locus_tag="PLY05_11380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HNC03940.1"
                     /translation="EELDDTLSKAWSLVSHLNAVTNSPAWRDAYNQCLAKISAYYTEI
                     GQNSKLYQSYQQLADSADFEQLSTAQQQTIKNALRDFKLSGVALADDKKQRFAEIEER
                     LSALSAKFADNLLDATQAWHKPLTDTELQGLPEAHQGLLAQLAQQRGLTGHVATLDIP
                     AYLAIMTYANDRALRQEVYTAYVTRASELSADGKFDNSAFMAEILSLRYEQAQLLGYD
                     NYAQLSLVPKMASSVDVVMQFLQELAQRAKPAAQQDLAELREFAATLGINDLQPWDTS
                     YVSEKLKQQKHNLSQEVLRAYFPAPMVIDGLFKIAERLYGVDIRAQQVDVWAEDVTYY
                     TISEQGKTIASFYLDPYARANKRGGAWMSNCQSRRATQLPIAFLTCNFNPPVGDKPAL
                     LTHDEVITLFHEFGHGLHHMLTEVDVTAVGGIHGVAWDAVELPSQFMEHWCWEEQALA
                     LMTQHIETGEALPKALLDNLLAAKNFQSGLQTLRQLEFALFDLRIHSQAPLTAQGIQD
                     TLNAVRAEVSVMSPPAFNRFQHSFSHIFAGGYAAGYYSYKWAEVLSSDAFSRFEQEGI
                     FNRETGLSFRQ"
BASE COUNT          473 a          369 c          390 g          503 t
ORIGIN      
        1 ttttgtctaa acgataagcc tgtttcgcgg ttaaaaatac cttcttgttc aaagcgtgaa
       61 aaggcatcgc tcgacaacac ttcggcccat ttatagctgt agtaacctgc ggcataacca
      121 cctgcaaaaa tatggctaaa actatgctga aaacgattaa aggcaggagg tgacatcaca
      181 ctcacttcgg cacgtaccgc atttaaggtg tcttgaatgc cttgtgcggt taaaggtgct
      241 tggctatgaa tacgcaaatc aaacagggca aactctaatt ggcgtaaggt ttgtaagcct
      301 gattgaaagt tttttgccgc taacaaattg tctaataatg ctttaggcaa ggcctcgcct
      361 gtctcgatat gttgagtcat taaggcaagg gcttgttctt cccaacacca atgttccata
      421 aattgactgg gtaactccac cgcatcccac gccacaccat gaatcccacc cacagccgtg
      481 acatcgactt cggtcagcat atgatgtaag ccatgaccaa actcatggaa caaggtaatc
      541 acttcatcat gagttaataa agcaggtttg tcgccaacag ggggattaaa gttacaggtt
      601 aaaaaggcaa tgggtagttg tgtcgcgcgg cgtgattggc aattgctcat ccatgcacca
      661 ccgcgtttat tggcgcgggc ataagggtct aaataaaatg aggcaatggt tttgccttgt
      721 tcactgatgg tgtaataggt aacatcttct gcccaaacat caacttgttg tgcgcgaatg
      781 tctacgccat acaaacgttc agcaatttta aataaaccat caatcaccat cggcgcaggg
      841 aaatatgctc ttaatacttc ttgcgacaaa ttgtgttttt gctgtttgag tttttccgag
      901 acataactgg tgtcccacgg ttgcaaatca ttgattccca acgttgcagc aaattcgcgt
      961 aattcggcta agtcttgttg tgccgctggt ttggcgcgtt gcgccaactc ttgtaaaaac
     1021 tgcatgacca catcaaccga agaagccatt tttggcacta aagacaattg tgcgtagttg
     1081 tcataaccta ataattgcgc ttgctcataa cgcaaactta aaatttcggc catgaatgcg
     1141 gagttatcaa atttgccatc ggcacttaat tcagaggcgc gagttacata agcggtataa
     1201 acttcttggc gcaaagcacg gtcattggcg taagtcatta ttgctaaata cgcaggaata
     1261 tccaaagttg caacatgacc tgttaagccg cgttgttggg ctaattgtgc cagtaaacct
     1321 tgatgcgctt cgggtaagcc ttgtaattca gtgtcggtta agggtttatg ccatgcttgt
     1381 gtcgcatcta ataaattatc ggcaaactta gcactcaagg ctgataaacg ttcttcgatt
     1441 tcggcaaaac gttgtttttt gtcatcagct aaggccacac cactgagttt aaaatcgcgt
     1501 agggcgtttt taatggtttg ttgttgggcg gtgctgagtt gttcaaagtc ggcactgtcg
     1561 gctaattgtt gataactttg atagagtttg ctgttttggc caatttcggt gtaataggca
     1621 gaaattttgg ctaaacattg attataggca tcacgccaag caggactgtt ggtaacggca
     1681 tttaaatgac tgactaacga ccatgctttg ctgagtgtgt catctagttc ttcta
//
LOCUS       DAOQMB010000491         1729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_51056_length_1729_cov_13.489845, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000491 DAOQMB010000000
VERSION     DAOQMB010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51056_length_1729_cov_13.489845"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..508
                     /locus_tag="PLY05_11385"
     CDS             17..508
                     /locus_tag="PLY05_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HNC03941.1"
                     /translation="MTLPSPRLTNLAIFVFTCISMAFALYLQHYQNLEPCPLCIFQRT
                     AVIGIGVTALIAAIHNPAVLGRRIYAGLTILSAAWGLAVAGRHVWLQHLPADEVPACG
                     PGLDYWMDTFPLQEVIQKVFRGSGECAKVDWTFLGFSLPEWTLVMFVGYACLAIWQLV
                     RKD"
     gene            complement(541..1635)
                     /locus_tag="PLY05_11390"
     CDS             complement(541..1635)
                     /locus_tag="PLY05_11390"
                     /inference="COORDINATES: protein motif:HMM:NF039651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC03942.1"
                     /translation="MFKSLCQFAPAALLMSQAYAADLLPLAKDVKPNTKGLPAAFVKP
                     MKISVCFYDMQGSTGEFYSRAKDLGLIARRWNIIADFKVIPEEAKVAEAFKAGKCDAA
                     VLSTLRAKEFNKFMGSVDAVGAIPTYQHMRLLLKTLFDPKLEPLTISGPYQIVSIFPV
                     GAQYLHIKDHAVEDIGQLANKKVSVINWGDSLASAMQKVGFTPISGDVGEFTKPFNAN
                     EVDALVAPAMVYWPYELKQGLGEKGGIYSTPINQMTASLVINRELLQKKAPDIDNRVA
                     AFRAMTGQFMDEMLDKIFNTIDKNEKDVPKKYWMILEPQNEKKYQEALRQARIQLTKE
                     GVYDEKMMKVLKKIRCKIDANAPECSSNEE"
BASE COUNT          482 a          351 c          304 g          592 t
ORIGIN      
        1 ttttgagagc aaccacatga ctttaccctc tccacgttta acgaatttag ctatttttgt
       61 gtttacctgt attagcatgg cttttgcgtt gtatttacaa cattatcaaa atcttgaacc
      121 atgcccttta tgtatttttc aacgtaccgc agtgattggt attggcgtaa ctgccttaat
      181 tgccgctatt cataatcctg ctgtgttagg tcggcgtatt tatgcaggct taactatttt
      241 gtccgcagca tggggcttag cggttgcagg tcgtcatgtg tggttgcaac atttacctgc
      301 tgatgaagtg cctgcttgtg ggccgggttt ggactattgg atggacactt ttcctttaca
      361 ggaagtgatt caaaaagtct ttcgtggctc tggtgaatgc gccaaagttg attggacgtt
      421 tttaggcttt agtttacccg aatggacact ggtgatgttt gttggttatg cctgtttagc
      481 gatttggcag ttagttcgca aagattaaaa aacaaagggc ggtttaaccg cccttttgtt
      541 ttattcttcg ttgctactac actctggtgc attagcatca attttacaac gaattttctt
      601 taagactttc atcatctttt cgtcatacac accttctttg gttaattgaa tacgtgcctg
      661 acgtaaagct tcttgatatt ttttctcgtt ttgtggctct aaaatcatcc aatatttttt
      721 gggtacatct ttttcgttct tatcaatcgt attaaaaatc ttatcaagca tttcatccat
      781 aaattgacct gtcattgctc taaaagcagc aacacgatta tcaatatcag gggctttttt
      841 ctgtaataac tcacgattaa tcactaagct agcggtcatt tgattaatcg gtgtactata
      901 aatcccccct ttttcaccta aaccttgttt taattcataa ggccaataca ccattgcagg
      961 tgcaactaag gcatctacct cattggcatt aaatggctta gtaaactctc caacatctcc
     1021 actaattggc gtaaaaccga ctttttgcat ggcagaagct aaagaatccc cccaattaat
     1081 caccgagacc tttttgtttg ccaattggcc aatatcttct actgcatgat ctttaatatg
     1141 taaatattgc gcaccaacag ggaaaatact gacaatttga taaggcccag atatggttaa
     1201 tggctctaat ttgggatcaa acaaagtctt taataacaaa cgcatatgtt gataggtagg
     1261 aattgcccca accgcatcaa cactacccat aaatttatta aactctttcg ctctaagtgt
     1321 tgataatact gctgcatcac atttgcctgc cttaaacgct tcggcaactt ttgcttcttc
     1381 gggaatcact ttaaaatcgg caataatatt ccaacgacgc gcaattaacc ccaaatcttt
     1441 agcacgacta taaaactcac ctgttgaacc ctgcatatca taaaaacaga cagaaatttt
     1501 cattggtttg acaaaggcag ctggtaaacc tttggtatta ggcttcacat ctttagccaa
     1561 tggcaataaa tcggcagcat aagcttgtga cattaataag gcagcagggg caaattgaca
     1621 aagagactta aacataaatt ttcctttttt atcaaaagac taaccaagtc attgacagtt
     1681 ttatttgttg atttaaattt ttattaatat tgattgttat agtttgatt
//
LOCUS       DAOQMB010000492         1726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_51231_length_1726_cov_8.943148, whole genome shotgun sequence.
ACCESSION   DAOQMB010000492 DAOQMB010000000
VERSION     DAOQMB010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51231_length_1726_cov_8.943148"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="PLY05_11395"
     CDS             <1..668
                     /locus_tag="PLY05_11395"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNC03943.1"
                     /translation="DPKLVPRMVALDSVIMSGMPPKVTAETGLDALTHAVEAYISDFA
                     AEDSDLYARSAIKLIINNLRVAYKEPKNLAAREAMALASHYAGLAINLAGLGYVHAIA
                     HQLGGHYGLPHGLSNAIVLPYVLNFSRPVIDERLAELARFCGLVTQETSTAIAAQAFM
                     DELKQLMSDLNIKAVVPKMTPQDFDDIMEAAFKEAHGTYAVPKYMNEDDCLQMLYMIA
                     QAS"
     gene            complement(783..>1726)
                     /locus_tag="PLY05_11400"
     CDS             complement(783..>1726)
                     /locus_tag="PLY05_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HNC03944.1"
                     /translation="SKEKASSLHRQWLILQHLPQGKWMGTADLQLMLERAGVHVSLRT
                     IQRDLNQLAERFPLEGNGASPQGWRWRADAQISSLPHITTSQAVTFLMVEEHLRHLLP
                     PSLLDELRPWFDMAKRSVNHADNQGRHWLDCVRIIPPTQPLIPPTVNRQAQQAIYEGL
                     LANKQVKVDYQRRYDKVAAEYILNPLGIVQRGHVIYLICTRHDDAKIRLFALQRFNNA
                     EVLKSPVVIPKEFNLDDYLQTGALGFGEGESIRLKAKFTKVVGDHLYESKLSEDQVIT
                     ELDNEHLLIEATVKHTQQLVWWLRGFGDAVEVLEPVL"
BASE COUNT          488 a          355 c          363 g          520 t
ORIGIN      
        1 tagaccctaa attagttcct agaatggtcg ctttagattc ggtgattatg tcgggaatgc
       61 cacccaaagt gaccgctgaa acaggattgg atgctttaac gcatgctgta gaagcttata
      121 ttagtgactt tgctgctgaa gattctgatt tatatgcacg ttcagcaatt aaattgatta
      181 ttaacaattt acgtgttgct tataaagaac ccaaaaattt agccgcccgt gaagccatgg
      241 ccttagcatc gcattatgca ggattggcga ttaacttggc aggcttgggg tatgtgcatg
      301 ccattgccca tcaattaggc ggtcattatg gtttgccaca tggtttaagt aatgccatcg
      361 ttttgcctta tgtgttgaat ttttcgcgac ctgttattga tgagcgttta gctgagttgg
      421 cgcgtttttg tggtttagtg actcaggaaa cctctactgc cattgcagcg caagctttta
      481 tggatgagtt aaaacaatta atgagtgatt taaacattaa agccgttgtg ccaaaaatga
      541 caccacaaga ctttgatgac attatggaag ctgcttttaa agaggctcat ggcacttatg
      601 ctgtgcctaa atatatgaac gaggatgatt gtttgcaaat gttgtatatg atagctcagg
      661 cgagctagtg gggttactga gcgtagttga agtagtggtt tcgactacgc tcaaccacca
      721 caactccacc caacgcagat tttgtactaa gtgggcagag ttgcaatcat accaacaaaa
      781 ttttataaaa cgggctctaa cacctcaact gcatctccaa aaccacgtaa ccaccaaacc
      841 agttgttgtg tatgtttaac ggtggcttca attaataaat gttcattatc taattcagta
      901 atcacttggt cttcgctgag tttgctttca taaagatggt cgccaaccac tttggtaaat
      961 ttagctttta agcgaatact ttctccctcg ccaaaaccca atgcgcctgt ttgtaaataa
     1021 tcatctaaat taaactcttt aggaatcacc acaggacttt ttagcacctc ggcattatta
     1081 aaccgttgca gcgcaaataa acgaattttg gcatcatcat ggcgtgtaca aatcaaataa
     1141 attacgtggc cacgttgcac aatacctaaa ggatttaaaa tatattcagc cgcaactttg
     1201 tcataacggc gttggtaatc gactttgact tgtttattgg ctaataagcc ttcataaatc
     1261 gcttgttgtg cttggcgatt tactgttggc ggaatcaacg gttgtgtggg cggtataatg
     1321 cgtacacagt ctaaccaatg gcgaccttga ttgtcggcat gatttaccga acgtttagcc
     1381 atatcaaacc acggacgcaa ttcatctaat aaactagggg gtaataaatg gcgcaaatgt
     1441 tcttcgacca ttaaaaaagt taccgcttga ctggtggtaa tgtgtggcaa actggaaatt
     1501 tgcgcatcgg cacgccaacg ccaaccttgt ggacttgcgc cattgccttc gagtggaaaa
     1561 cgctcggcca attgatttaa atcacgttga atagtacgca aactaacatg cacgcctgca
     1621 cgttctagca tcagttgtaa atcggcagta cccatccatt tgccttgagg aagatgttgt
     1681 aaaattaacc attgtctatg caaactactg gctttttcct tgctca
//
LOCUS       DAOQMB010000493         1724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_51340_length_1724_cov_19.055722, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000493 DAOQMB010000000
VERSION     DAOQMB010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51340_length_1724_cov_19.055722"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /gene="amt"
                     /locus_tag="PLY05_11405"
     CDS             <1..1023
                     /gene="amt"
                     /locus_tag="PLY05_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC03945.1"
                     /translation="PIHEMIYIVFQGAFAAITCALIVGAFAERAKFAGILAFMVLWFT
                     FSYLPMAHMVWYWAGPDAYTDADAATAATATAGWLFQHGALDFAGGTVVHINAAVAGL
                     VGAYVIGKRVGYGREAMLPHNMVMTMIGAALLWFGWFGFNAGSALEANGIAGLAFLNT
                     WLATAAAAISWMATEWIFKSKPSMLGAASGAVAGLVAITPACGFVGAGGALAIGLISG
                     AVCLWGVHGLKKLLGADDALDVFGVHGVGGIIGALLTGVFAAPSLGGTGIWDYVANGV
                     AADYNMLAQVKVQAIGVVVTIVWSSVVAFVCYKLVDITIGLRVSEEEERIGLDVTSHG
                     ERAYND"
     gene            1107..1532
                     /locus_tag="PLY05_11410"
     CDS             1107..1532
                     /locus_tag="PLY05_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HNC03946.1"
                     /translation="MTKLLLGTIILFYCSFTHAEIYKWLDEHGNPQFSDEPPKQGQYK
                     KLNISPSPDISTSEDAPSTSSSMSGEMIKKQKAQQELAEKQQLLEQECLRAKDYLEAV
                     QTAKIYDLNNQGERVYKSDQERANEIKRVERAIKLHCPQ"
BASE COUNT          456 a          345 c          383 g          540 t
ORIGIN      
        1 ccaattcacg aaatgattta tatcgtgttc caaggtgctt ttgccgcaat tacttgtgca
       61 ttgattgtcg gtgcttttgc tgagcgtgca aaatttgcag gtatcttagc ctttatggtg
      121 ttgtggttca ccttctctta tttaccaatg gcacacatgg tttggtactg ggcaggtcca
      181 gacgcctata ctgatgctga tgctgctact gccgctactg caactgctgg ctggttattc
      241 caacatggtg ctttagattt tgcaggtggt acagttgttc atattaatgc tgctgttgca
      301 ggcttagtag gtgcttatgt gattggcaaa cgtgttggtt atggccgtga agcgatgttg
      361 ccacataaca tggtgatgac catgattggt gcagctttat tgtggtttgg ttggtttggt
      421 tttaatgctg gctctgcttt agaagccaac ggtattgctg gtttagcctt cctcaacaca
      481 tggttagcca ctgctgctgc tgcgatttca tggatggcca ctgaatggat cttcaaaagc
      541 aagccttcta tgttaggtgc tgcttctggt gcagttgctg gtttggttgc cattacacca
      601 gcttgcggtt ttgttggcgc aggtggtgct ttagcgattg gtttaatctc tggtgctgtt
      661 tgcttatggg gcgtacatgg tttgaagaag ctcttaggtg ctgacgatgc cttagatgtg
      721 tttggtgtac acggtgttgg tggtatcatt ggtgcgcttt taacaggtgt gtttgctgct
      781 ccatctttag gtggcacagg catctgggat tatgtggcta atggtgttgc tgccgactac
      841 aacatgcttg ctcaagttaa agtacaagca attggtgttg tagtcactat cgtgtggtcg
      901 agtgtggttg ccttcgtttg ctacaaatta gttgatatta ctatcggctt acgtgtcagc
      961 gaagaagaag agcgtatcgg cttagacgta acatctcacg gtgaacgcgc atacaacgac
     1021 taatttttaa ttagtcaaca aaaacggagg caaaagcctc cgttttttat ttatacttag
     1081 agctaatggc aaataggtct aagtgtatga ctaaattatt attagggaca atcatcctat
     1141 tttactgttc tttcactcat gccgaaattt ataaatggct tgatgaacat ggtaatcctc
     1201 aatttagtga tgaaccgcct aaacagggtc aatataaaaa actaaatatc tccccatcac
     1261 ctgatatatc tacatccgaa gacgcccctt caacatcttc ttcgatgagc ggtgaaatga
     1321 taaaaaaaca aaaagctcaa caagagcttg ccgaaaaaca acaattatta gaacaagaat
     1381 gccttcgagc caaagattat ctagaagctg ttcaaactgc caaaatctat gatttaaata
     1441 atcaaggtga acgagtttat aaatcagatc aagaacgtgc taacgaaatt aaacgtgttg
     1501 aacgcgctat caaactacat tgtccgcaat agcttccttc gtaaccacaa ttaaaacatc
     1561 acagccccta atatgtcaca accatttctc ccctctttaa ataacgaagc atccacttgg
     1621 ataaatcaac aactacgcgc attacccact aaaaatattg aaaaaggcca acgtctctac
     1681 accgaaggcc agcattttga tacggtttat attaccttgt ctgg
//
LOCUS       DAOQMB010000494         1723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_51399_length_1723_cov_3.923861, whole genome shotgun sequence.
ACCESSION   DAOQMB010000494 DAOQMB010000000
VERSION     DAOQMB010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51399_length_1723_cov_3.923861"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1390)
                     /locus_tag="PLY05_11415"
     CDS             complement(233..1390)
                     /locus_tag="PLY05_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03947.1"
                     /translation="MGVIKKTALSIAVSVMLAACGGGDDNTGNTGNTGNQTEVPFTPK
                     KLSHWEHAIIDEDTVYLQRDDGTLWKWPLVAAGKPQRIDKVDDAIKSIEKRNITFSEN
                     EIFAITQSGNVYKIDKVKGVLTSVATNVKQIATNMYLQNDGTVRDWSHNSILNIPKME
                     RLLESDLNFEGAYAQAGIGKDGLLYYFKGIESTASGESAVLGASIVGAYPDAQAVLSV
                     KDVGAERVLAILQAGNKWLILDNAGKVVNEQKFSGVINTRGVSGRDVSRLISYPGPTV
                     ITSDGQSWKWSDSKSLFVLQSECAGAQMLLPSGLLFMQNGSFLEYSKGGLSGNEYCYE
                     QSGHLKDVPVKDALGNWLTVVGVKSDDGLIASVRKIGQSPAFQTFLADKPM"
     gene            1533..>1723
                     /locus_tag="PLY05_11420"
     CDS             1533..>1723
                     /locus_tag="PLY05_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03948.1"
                     /translation="MCQCTPEIRTPYCVNCAFPEHTISLPKIDKIPPMPPMPPVVKPP
                     LCNLRAFYEAAGQHLSHAT"
BASE COUNT          529 a          406 c          287 g          501 t
ORIGIN      
        1 caagaaaaga acggtcaaaa cacaaactta ctttaaattt actacagtgc aataactaaa
       61 accaaaatca gaacacaagt taaggcctaa ctactagcgt aaggcgcaag caccctacac
      121 gagcctaata aagaaaaccg taatcacgac tttcaaaaat tgcacaataa tcggctgggt
      181 gcttgtcgca cttgacgcaa aagttaggca ttgactaacc atattggttt tatcacatag
      241 gcttatcagc caaaaaagtc tgaaaagctg gagattgacc aatttttcta acactggcga
      301 ttaaaccatc gtcagattta accccaacaa cagttaacca attacctaaa gcatctttca
      361 ctggaacgtc ttttaaatgc cctgattgtt catagcagta ttcgttgcca ctgagtccac
      421 cttttgaata ctctaaaaag ctgccgtttt gcataaaaag caagccgcta ggcaataaca
      481 tttgtgcgcc tgcacattca gactgtaaaa caaataagct tttggaatca ctccacttcc
      541 atgattgccc gtcactcgta ataacagttg ggcctggata cgaaataagc cttgagacat
      601 cacgcccact cacgccacgt gtgttaatta caccagaaaa cttctgttca ttgacaacct
      661 tacctgcatt atccaaaata agccatttgt tgcctgcttg taatatagct aaaactcgct
      721 cagcaccaac atcctttact gataagacag cctgtgcatc aggatatgct ccaacgatag
      781 aagcacctaa aactgctgac tcaccagatg cggtgctctc aatacctttg aagtaatata
      841 gcaaaccatc tttgccaatc cctgcttggg cgtaagctcc ctcaaaattc aaatcactct
      901 ctagcaatcg ctccattttt ggaatattaa gaattgaatt atgactccaa tctcgcactg
      961 taccgtcatt ttgtaagtac atattggtag cgatttgctt tacattagtt gcgaccgacg
     1021 taagcactcc ttttacttta tctattttat agacattgcc actctgtgtt atggcaaaaa
     1081 tctcattttc ggaaaaagta atattgcgtt tttctatgga cttaatcgca tcatcaactt
     1141 tatcaattct ttgtggcttt cctgctgcta caagcggcca tttccataat gtcccatcat
     1201 ctcgctgtaa ataaacagta tcctcatcaa ttattgcatg ttcccaatgg cttagttttt
     1261 taggtgtaaa tggtacttct gtttgattgc ctgtattgcc tgtattgcct gtattgtcgt
     1321 caccaccacc acaggccgca agcattactg aaacagcaat tgataaagct gtttttttaa
     1381 ttacacccat aataactcct ttatttatta agccagcaaa atgcctaact aaaaattcag
     1441 gcgcaagcac cgcgctgaca gcaaacctaa aatacagttc gggtgcttgt cgcgctgtaa
     1501 tgagagttag atacaaataa gaggtgtttg atatgtgcca atgtacgcca gagattagaa
     1561 ctccgtattg tgttaattgt gcttttccag agcatacaat cagtcttcca aagattgata
     1621 aaattccacc catgccaccc atgccgccag ttgttaaacc acctttgtgt aatttgcgag
     1681 ccttttatga agcagcaggg caacacctta gtcatgcaac att
//
LOCUS       DAOQMB010000495         1711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_52078_length_1711_cov_18.837560, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000495 DAOQMB010000000
VERSION     DAOQMB010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52078_length_1711_cov_18.837560"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..147
                     /locus_tag="PLY05_11425"
     CDS             25..147
                     /locus_tag="PLY05_11425"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNC03949.1"
                     /translation="MSWCDNHLKGGFGIMFDDVLAGLAAGGVMWFIVSGGYLAL"
     gene            111..782
                     /locus_tag="PLY05_11430"
     CDS             111..782
                     /locus_tag="PLY05_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HNC03950.1"
                     /translation="MVYRQRRLFSIVISGLLANISYGQEVKVLGLFGDRAFIKVDEKS
                     AVIRIGQSHQNVQLKSISNQKAILLVDNKDIALGLGDSYQSAPPVVEEKKPEVVKPKA
                     SVTIYSNLGHQFITSGVINGRIVSFVVDTGATSISMSRKHAQRLGIDFRTKGKEGLSS
                     TANGDVKHWQMMLNSVKVGAITLSFVEATIRDTDDDLPILLGMSFLSRVKLEQSGQKM
                     VITEK"
     gene            983..1567
                     /gene="ribA"
                     /locus_tag="PLY05_11435"
     CDS             983..1567
                     /gene="ribA"
                     /locus_tag="PLY05_11435"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009513805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HNC03951.1"
                     /translation="MSIVFIARSKLPTKHGDFIIHAFEETDSSQEHIALTMGDIADGQ
                     PVLARIHSECLTGDGFGSLRCDCGPQLEAAMQHIAQTGRGVILYLRQEGRGIGLVNKV
                     RAYALQDQGADTVEANEQLGFAPDLREYSMCEYMLAHLGVKQVRLMTNNPLKVAALEQ
                     HNIHVVERVPLMTGRNPHNDQYLDTKEEKMGHLF"
BASE COUNT          506 a          264 c          385 g          556 t
ORIGIN      
        1 tttgatattc tcaaaccgtg gccaatatca tggtgcgata atcacctaaa aggtggtttt
       61 ggcattatgt ttgatgatgt gttagcagga ttagccgcag gaggtgtgat gtggtttatc
      121 gtcagcggcg gttatttagc attgtaataa gtggtttatt agctaatatc agctatggcc
      181 aagaagtcaa agtactaggc ttatttggtg atagagcttt tattaaagtt gatgaaaaaa
      241 gtgccgttat tcgcattggc caaagtcatc aaaatgtaca acttaaatcg attagtaatc
      301 aaaaagcgat attactggtt gataataaag atattgcttt ggggctaggt gattcctatc
      361 aatcagcacc tcctgtggtt gaagaaaaaa agcccgaagt tgttaagcct aaagcgagtg
      421 tgacgatata tagtaattta ggtcatcaat ttattaccag tggtgtaatt aacggtcgta
      481 tagtttcttt tgtggttgat acaggtgcaa cgtccatttc gatgagtcgt aaacacgccc
      541 aacgtttagg tatagatttt cgtactaaag gcaaagaagg gctaagtagt acggcaaatg
      601 gagatgttaa acattggcag atgatgctta atagtgtcaa agttggggca attactttgt
      661 catttgttga ggcgacaatt cgtgatactg atgatgattt accaatttta ttgggaatga
      721 gttttttaag tcgtgtaaaa ctagaacaat ctggccaaaa aatggtaata actgaaaagt
      781 agtgaatata ggctttattt gtgacgcaaa atagttgtaa ataaagcggt ttgtacgcca
      841 gttcgctatt gttgtcgatt gttttttgcg taattgtcct tatatagact aaaatctctt
      901 tctcaactag gacggatacc gtcatcaatg cattattttt gctgatcatg tatctgttct
      961 actaacctca gagagtctat tggtgtctat cgtatttatt gctcgttcta aattaccgac
     1021 taaacatggt gattttatta tccatgcgtt tgaagaaact gattctagcc aagagcatat
     1081 tgccttaacg atgggagata ttgctgatgg acagcctgtt ttagctcgaa tacattctga
     1141 atgtttaaca ggtgatggtt ttggttcttt acgttgtgat tgtggcccac aattagaagc
     1201 ggctatgcaa catattgccc agacaggtcg tggtgtgatt ttgtatttgc gtcaagaagg
     1261 gcgaggcatt ggtttggtca acaaagttcg agcttatgcg ttgcaagatc aaggtgctga
     1321 cacagttgaa gctaatgaac aattaggttt tgcacctgat ttgcgggaat atagcatgtg
     1381 tgaatatatg ttggcgcatt taggtgtaaa acaagttcgt ttgatgacca ataatcctct
     1441 caaagtcgct gctttagagc aacataacat tcatgtggtt gagcgtgtgc cgcttatgac
     1501 aggccgaaat cctcataacg atcaatactt agataccaaa gaagaaaaga tgggacacct
     1561 tttctaaggg atatttagat aatctcgtta cgattatgta ataagatttg acagttatat
     1621 gattagcggt aaaaataaga atcattctta tttatatttt atgttgaggc catagcatag
     1681 tgcgcctttc tcagttagcc aaaaacacgc c
//
LOCUS       DAOQMB010000496         1700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_52743_length_1700_cov_5.791489, whole genome shotgun sequence.
ACCESSION   DAOQMB010000496 DAOQMB010000000
VERSION     DAOQMB010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52743_length_1700_cov_5.791489"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="PLY05_11440"
     CDS             complement(<1..325)
                     /locus_tag="PLY05_11440"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC03952.1"
                     /translation="MDISKGQRTPISQLVTGQSFQLAVSTVGALVVDISCFGLDAAQK
                     LSDERYMTFFNQPKTPCGGVAIVQSTGKDTTTFHLNLALLPASIDRLVITAAIDGTGT
                     MNQLRD"
     gene            complement(328..1632)
                     /locus_tag="PLY05_11445"
     CDS             complement(328..1632)
                     /locus_tag="PLY05_11445"
                     /inference="COORDINATES: protein motif:HMM:NF021641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03953.1"
                     /translation="MDMIRGQKLKIADVTSGALQFVVNLQLVSSLTIDVSCFGVDSQG
                     KLSDERYMTFFNQPQSPCGGVKFVNNPNNQSSFQIDLTKIPASIDKLVFTAAIDGTGT
                     MGQLQASSLTINQGSSCRFAFSGQDFAAERALMVAEIYRKDGVWRIAAVGQGFNGGLD
                     ALVKHFGGEVAEAAPTATVAPTPTKANLTKRVQLEKRIEQEAPQLVSLIKKAQVNLEK
                     KGLGEHRAKVCLCLDISASMSQLYSSGKIQAFVERIVALACRFDDDGEVDVFLFGQKS
                     HHAPVITLRNYQQYIGHLIEHFPLEGGTHYSPVMKMIRQHYLQDDSDKKTPIHTPAEQ
                     PVYVMFVTDGATSNRKAAENQMLYASYQPIFWQFMGIGRDGSFEFLEKLDDLTGRYVD
                     NADFFSVKTPQDYDDDQLFARLMNEYPKWLEQAKQKQLVVRA"
BASE COUNT          504 a          370 c          307 g          519 t
ORIGIN      
        1 cgtcacgtaa ttgattcata gtgcctgtgc catcaatcgc ggcggtaatc actaatcggt
       61 caatcgaggc aggtaataac gctaaattta agtgaaaggt ggtggtgtct ttgcctgttg
      121 attgcacaat cgctacacca ccacaaggcg ttttaggttg attaaaaaag gtcatatatc
      181 gttcatcgct gagcttttgt gcggcatcta agccaaaaca gctaatatca accactaatg
      241 cacccaccgt gctaaccgct agttgaaagc tttgacctgt cactaattgg ctaatcggtg
      301 tacgttgtcc tttgctaatg tccattttta tgctctcacc actaattgtt tttgtttagc
      361 ttgttctagc catttgggat attcattcat taaccgcgca aataattggt cgtcatcata
      421 gtcttgaggg gttttaacac taaaaaaatc ggcgttatca acataacgac ctgttaagtc
      481 atccaatttt tctaaaaact caaatgaacc atctcgacct atgcccataa attgccaaaa
      541 aatcggctga tacgaagcat ataacatttg attctcggcc gctttacgat tgcttgttgc
      601 gccatcagtg acaaacatca catagacagg ttgttcggca ggcgtatgaa ttggtgtttt
      661 tttatcacta tcatcttgta aataatgttg gcgaatcatt ttcatcacag gtgaataatg
      721 tgtgccacct tctaaaggaa aatgctcaat taaatgacca atatattgtt gataattgcg
      781 caaggtaatc acaggtgcat gatgcgattt ttggccaaat aaaaacacat ccacttcacc
      841 atcatcatca aaacgacacg ctaaagccac aattctttca acaaaagctt gaatttttcc
      901 tgaactatac aattggctca tggatgctga aatatctaaa cataaacaca ctttggctcg
      961 atgttcgcct aagccttttt tctctaaatt aacttgggct tttttaatta agctgactaa
     1021 ttgcggagct tcttgctcaa tacgtttttc taattgcaca cgtttagtca agtttgcttt
     1081 tgtgggggtt ggtgcgacag tggctgttgg tgcagcttca gccacttcac ccccaaaatg
     1141 ttttactaaa gcatccaaac caccattaaa accttgacct accgccgcaa tacgccaaac
     1201 accgtcttta cggtaaattt cggcgaccat taacgcgcgt tcagccgcaa aatcttgacc
     1261 actaaaggca aaacgacaac tagaaccttg attaatggtt aatgaactgg cttgtaattg
     1321 tcccattgtg cctgtgccat caatggcagc ggtaaagact aatttatcaa tactggcagg
     1381 aattttagtt aaatctatct gaaaactgct ttgattattt ggattattaa caaatttgac
     1441 accaccacaa ggtgattgtg gctgattaaa aaaggtcata tatcgctcat cactgagctt
     1501 gccctgacta tcaacaccaa aacaactcac atctatagtt aatgagctaa ctaattgtaa
     1561 attcactaca aactgtaatg caccagaggt gacatcagca attttgagtt tttggccacg
     1621 aatcatatcc atgttgatgc tcctaaaggc tttgttgcac aaattacggt ttaggcataa
     1681 gttgccctta cccgataatg
//
LOCUS       DAOQMB010000497         1696 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_52983_length_1696_cov_4.471054, whole genome shotgun sequence.
ACCESSION   DAOQMB010000497 DAOQMB010000000
VERSION     DAOQMB010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52983_length_1696_cov_4.471054"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..1337)
                     /locus_tag="PLY05_11450"
     CDS             complement(183..1337)
                     /locus_tag="PLY05_11450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HNC03954.1"
                     /translation="MSSYSKIFALSATLISPFAQATTVIPAPPVLENKAFVLMDYDSG
                     KVLAESNAHAHLAPASLTKMMTSFLVEQRLLKGQLKEEDQVLMSENAWCRGTSKESCM
                     YVPLNSYASVLDMLRGIIIQSGNDASKAVAEHLAGSETAFAELMNGEAKRLGMKDTQF
                     KNATGLTEEGHYTSAYDMALLAHAIIKDSSKYYPIYSEKEFTYNNIKQGNRNALLFTD
                     PTVDGLKTGHTDEAGYCLTASSKRGSMRLISVVMGTPSMQARADQTRALFNWGFSNFE
                     TAQIRPKGQELAKPVVWFGQAENVSVGLASDMNVTLARGEKDKLQISLQVPSSLDAPI
                     KQGQEVGKIIATLDGKTVGEQALVALQPVEQAGFFTRVWHGIKRFFANLF"
     gene            complement(1469..>1696)
                     /locus_tag="PLY05_11455"
     CDS             complement(1469..>1696)
                     /locus_tag="PLY05_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HNC03955.1"
                     /translation="IPLWIDAALGQALSLNPEQRYADLSEFLHDLERPNPHLTLKSKP
                     WLEQNPLLFWQITCLLLLISNVIILFAWLKR"
BASE COUNT          532 a          327 c          329 g          508 t
ORIGIN      
        1 acttgcagta taaatactac ctatcaacgt gtcctgcttt attagaccat tacaagttgc
       61 tcaggtcata tcaacctgaa caactatatg aaatggcaaa aatgctacct ctccacttat
      121 gacataggta tctaaaaaat gtgtagatac ctttgcggct taggagaggg atgtttaatg
      181 cattaaaaca aattagcaaa aaagcgttta ataccgtgcc aaacacgagt aaagaagcct
      241 gcttgttcaa caggttgtaa ggcaactaaa gcttgttcac caacagtctt accatctaaa
      301 gtggcaataa ttttacccac ttcttgacct tgtttaatgg gggcatctaa agagctaggt
      361 acttgaaggc taatttgtaa tttgtctttt tcaccacgcg ctaaagtaac attcatatcg
      421 ctggctaaac cgactgaaac gttttctgcc tgaccaaacc aaaccacagg tttggctaat
      481 tcttggcctt taggacgaat ttgtgcagtt tcaaaattgc taaagcccca gttaaataag
      541 gcacgagttt ggtcagcacg agcttgcata ctcggtgtac ccatcaccac cgaaattaaa
      601 cgcatagagc cgcgtttgct agaagcagtt aagcaataac ctgcttcatc ggtgtggcct
      661 gtttttaaac catcaacagt agggtcggtg aataataagg cattacgatt gccttgtttg
      721 atgttgttgt aggtaaactc tttttcagaa taaatggggt aatatttgct gctatcttta
      781 ataatggcat gggctaataa agccatatca taagctgagg tataatgacc ttcttcggtg
      841 aggcctgtag catttttgaa ttgggtgtct ttcataccca aacgtttggc ttcaccattc
      901 attaattcgg caaaggcggt ttcgctgcct gccaaatgtt ccgcaacggc tttagaagca
      961 tcattacctg attgaataat aatgccacgt agcatatcta atactgaagc ataactattg
     1021 agtggcacat acatgcaaga ttctttgctt gtaccacgac accaagcatt ttcgctcatt
     1081 aacacttggt cttcttcttt aagctggcct tttaataaac gttgttcaac caaaaagctg
     1141 gtcatcattt tggttaaaga agcaggggct aaatgggcat gagcgttaga ttcggctaac
     1201 actttgcccg aatcataatc cattaataca aacgctttat tttctaaaac agggggagca
     1261 ggaataaccg ttgttgcttg ggcgaaaggg ctaattaaag ttgcactgag ggcaaaaatt
     1321 ttgctgtagc tagacataac acaaagccta taaataaagt agaaccataa tttttaaaaa
     1381 ttatggttct gtaagatgcc taaattaaat tgaggcgaat cttagcatta ttcatggctt
     1441 agttttgtat aacttgcttg acaaaaaact acctttttag ccatgcaaat aaaataatga
     1501 cattacttat caataataaa agacaggtaa tttgccaaaa taacaaagga ttttgctcta
     1561 gccacggttt actttttaag gtcaaatggg ggttagggcg ttctaagtca tgtaaaaatt
     1621 cacttaaatc agcataacgt tgttctgggt ttagacttaa agcttggcca agtgcagcat
     1681 caatccacaa aggaat
//
LOCUS       DAOQMB010000498         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53509_length_1687_cov_18.312500, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000498 DAOQMB010000000
VERSION     DAOQMB010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53509_length_1687_cov_18.312500"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1370
                     /locus_tag="PLY05_11460"
     CDS             168..1370
                     /locus_tag="PLY05_11460"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HNC03956.1"
                     /translation="MALLPLQESPSNQNGFWQILLSFGFRLFFLLAGLAAVIMVLLWL
                     AALNGWSSLLLIKHYGIHWHSHEMLFGYTTAVVAGFLLTAAKNWTGQHTAIGKPLMVL
                     GLCWLLARLLMWFDVALLQWIAIICDVLFLPLLAYFVARPILAIKQKRNYIFPVLLIL
                     MMLDHLLMHFALFTNQAVLIQKTIHAVLFIIMTIMVIMACRVVPFFTERGLSLKDPIK
                     RDERHDLWCAIAVAVAGVFYVVTTHWLTTAFLWLAAVTLVSRTIKWYQPKVWQVPLLW
                     VLHVGMLWLALGLVLLGWQNLPLATHMGSSGIHALTIGCIGMMTLGMMSRVSLGHSGR
                     ALQTVKATHWGFILLNLAAICRVFGAWAFSSHYVLMVTVAGVLWISAFLLFLWVYVPI
                     FITPSKSM"
     gene            1374..>1687
                     /locus_tag="PLY05_11465"
     CDS             1374..>1687
                     /locus_tag="PLY05_11465"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HNC03957.1"
                     /translation="MNLLLLTPQDCPQANIAHLTGRRLQHVLEVHQAKIGQKLRVGMV
                     NGLMGEAEITHLDKQLLQLDFVLNESPPAKLPLTLLLALPRPKMLKRILQTVATMGVQ
                     RLV"
BASE COUNT          410 a          302 c          391 g          584 t
ORIGIN      
        1 tgggcaatct tgttgaagca ggaagtaaac acaaagccta tttgttctac aataaatagg
       61 ttttgataag ttttatggaa cggtgatgct ttttagtatc aatccttaaa taaagaagac
      121 aaatcacccg caatatgtgg cttgttattt tatggtttga atgaattatg gcattacttc
      181 ctttgcaaga atcaccctca aatcaaaatg gattttggca aatattattg agctttggtt
      241 ttagactgtt ttttttattg gcaggattag ccgccgttat catggtttta ttatggctag
      301 ctgcgcttaa tggttggtcg tcgctattat tgattaagca ttatggaatc cattggcata
      361 gccacgaaat gctctttggc tataccaccg ccgttgtggc aggtttttta ctgaccgccg
      421 ccaaaaactg gacaggccaa cataccgcta ttggtaagcc attgatggtg ttaggcttat
      481 gttggttgtt ggcacgattg cttatgtggt ttgatgtggc tctattacaa tggattgcta
      541 ttatatgtga tgtgttgttt ttgcctttgt tagcctattt tgtggcacga cccattttgg
      601 ctattaagca aaaacgtaat tatatttttc ctgtgttgtt aatccttatg atgcttgacc
      661 atctgttaat gcactttgct ttatttacta atcaagcggt tttaattcaa aaaacaattc
      721 atgccgtgtt gtttattatt atgacaatca tggtcattat ggcgtgtcga gtagtccctt
      781 tttttactga gcgtggcctt agtctaaaag accccattaa acgtgatgaa cgccatgatt
      841 tatggtgtgc gattgcggta gccgttgcag gggtgtttta tgtagtgact acgcattggt
      901 taacaacggc ctttttgtgg ttagccgccg taacattggt tagtcgaacc attaaatggt
      961 atcagcctaa agtgtggcaa gtacctttgt tatgggtgtt acatgtgggt atgttgtggt
     1021 tagcactagg tttggtgttg ttgggatggc aaaatctacc acttgctacg cacatgggta
     1081 gtagtggtat tcatgcctta actataggct gtattggcat gatgacttta ggcatgatgt
     1141 cgcgcgtgtc gttagggcat agtggccgcg ccttacaaac agttaaggca acacattggg
     1201 gctttatttt gctcaatctg gccgcaatat gtagagtatt tggtgcgtgg gcttttagca
     1261 gtcattatgt attaatggta acggttgcag gtgtattgtg gattagtgca tttttgttgt
     1321 ttttatgggt ttatgtcccc atttttatca ctccctctaa gtctatgtag cttatgaacc
     1381 tcctcctact cacccctcaa gattgccctc aagccaacat tgctcattta acgggtcggc
     1441 ggttacaaca cgttttagaa gtgcatcaag ctaaaattgg ccaaaaacta cgggtaggca
     1501 tggtcaatgg gttgatggga gaagccgaaa ttacgcattt agataagcaa ttgctacagc
     1561 tagattttgt actgaatgag tcgccgcctg caaagttgcc tttaacttta ttgttagctt
     1621 tacctcgccc taaaatgctt aaacgcattt tgcaaacggt ggccacgatg ggcgtacagc
     1681 gtttggt
//
LOCUS       DAOQMB010000499         1687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53510_length_1687_cov_11.011642, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000499 DAOQMB010000000
VERSION     DAOQMB010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53510_length_1687_cov_11.011642"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..939
                     /locus_tag="PLY05_11470"
     CDS             115..939
                     /locus_tag="PLY05_11470"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNC03958.1"
                     /translation="MANILLIGCGDVGLRLANKLISIGHQLTIIKRSPLELAGATVIN
                     QDVSQSFSLSSLSPDYVFIILSPSASTPQAYQQVFIDGLKNIAQALTHHQPKRVFFVS
                     SSSVYGQDAGQWVDEQTPPEVAGFNGQILLQAEALCLQTWPSTILRCAGIYGEGRLRL
                     INWLKAAKPVETGLWSNRIHIDDVVGILLYLLAVAQTEQSLSPVYLGVDDCPVLQAEV
                     LDWLAEQLALTPAPKVNTAPRNKRLSNQAIKQLGYVFKYPSFKEGYGGMLEMSKEP"
     gene            complement(936..1667)
                     /locus_tag="PLY05_11475"
     CDS             complement(936..1667)
                     /locus_tag="PLY05_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016539658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03959.1"
                     /translation="MTISGFVAGTLVHTDKGLVPIEQLKVGDMVLSKHESGQGEKAYK
                     RVISTFKSAEKTLISYINYCVIDEDSKISSAINRFLFCTENHLFWTCDDNDDLHGWTI
                     AKSIGFLDGVFIFVLHDNQMAFNLSPIGWENSVFEQDNSKIALVSSDGDDPIAIYDFN
                     SGQPVCLGGRIYTNLHPSEAKSSQDMELDSRIMHLYETGLSHPYLDDVYNLEVEDFHT
                     YYVGKAGIWTHDATDIATHLRPIED"
BASE COUNT          480 a          310 c          359 g          538 t
ORIGIN      
        1 caaaatggcg caattcggca agagtgacta aatagcgtag gtcggttagg gtcatagttt
       61 ttaagaatta gtttttaaat attgattgtt ttagtttatg agtgaatagt gaaaatggca
      121 aatatcttgt taattggctg tggtgatgtt ggtttacgtt tggctaacaa gctaattagt
      181 atcggtcatc agctaacgat tatcaaacgt tcacctttag agttggcagg tgcaacggtg
      241 attaatcaag atgtgagtca gtcttttagc ttgtcctcgt tatcgcctga ttatgtcttt
      301 attattttaa gtccaagtgc atcaaccccc caagcctacc aacaagtgtt tattgatggc
      361 ctaaaaaata ttgctcaagc cttgacgcat catcagccta aacgcgtgtt ttttgtctca
      421 tcaagcagtg tgtatggaca ggatgcaggg cagtgggtcg atgaacaaac gccgcccgaa
      481 gtggcaggtt ttaatggtca gattttgttg caggcagaag ccttatgttt gcaaacatgg
      541 ccaagcacga tattacgatg tgcaggtatt tatggtgaag gacgtttgcg tttaattaat
      601 tggctaaaag cggcaaaacc tgtagaaaca ggcttgtggt caaatcggat tcatattgat
      661 gatgtggtgg gcattttgtt gtatttgttg gcagttgccc aaactgagca aagcttatca
      721 cctgtttatt tgggggtgga tgattgtcct gttttacaag ccgaggtatt ggattggttg
      781 gcggagcagt tggctttaac gcctgcccct aaagttaata ctgcaccacg caataaacgt
      841 ttgagtaatc aagcaattaa acaattaggc tatgttttta aatatccaag ttttaaagag
      901 gggtatgggg ggatgctaga gatgtcaaaa gagccttagt cttcaatcgg ccttagatgt
      961 gtggcaatat ctgtggcatc gtgagtccaa atacctgctt tgccgacata atatgtgtga
     1021 aaatcttcta cttcgaggtt gtaaacatca tctaaatacg ggtggctcaa tcctgtttcg
     1081 tataagtgca ttatccttga atctaattcc atatcttgag aagattttgc ttctgatggg
     1141 tgtaagtttg tatagatacg tccaccaaga caaacaggct gaccactatt aaagtcataa
     1201 atagcaatag ggtcatcgcc atcactcgaa accaaagcga tttttgagtt atcttgttca
     1261 aatacgctgt tttcccatcc tataggagat aaattaaaag ccatttgatt atcatgcaat
     1321 acaaatataa aaactccatc aagaaagcct atgctttttg caatcgtcca gccgtgaagg
     1381 tcatcattgt catcacatgt ccaaaaaaga tggttttctg tgcaaaataa aaatcggttt
     1441 attgcgcttg agattttgct atcctcatct atgacacaat aatttatata gctaatgagt
     1501 gttttctcag cagatttaaa agtactaatc acacgtttat aggccttctc gccttgccca
     1561 ctttcatgct tcgacagcac catatccccc acttttaact gctcaatggg gactaaacct
     1621 ttatcggtat ggactaatgt gcctgccaca aatcctgaaa ttgtcatttt ttgtccttaa
     1681 taatgta
//
LOCUS       DAOQMB010000500         1686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53642_length_1686_cov_1.744329, whole genome shotgun sequence.
ACCESSION   DAOQMB010000500 DAOQMB010000000
VERSION     DAOQMB010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53642_length_1686_cov_1.744329"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..153)
                     /locus_tag="PLY05_11480"
     CDS             complement(19..153)
                     /locus_tag="PLY05_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03960.1"
                     /translation="MVALMRASLKETVHQTKSAHAGLLLSHSLPIWEQNEKTEKCHYH
                     "
     gene            complement(155..502)
                     /gene="cmr5"
                     /locus_tag="PLY05_11485"
     CDS             complement(155..502)
                     /gene="cmr5"
                     /locus_tag="PLY05_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HNC03961.1"
                     /translation="METRDQKYAKAAYPKVDYQRGKDTEKGYRTLALNFPVMILQSGL
                     TQSIGFLLAKNKVEHQAYLKDLAEVLGLQNANNLHQTIINSNLNDYQQHTRKALEAAA
                     WLKRYTQALLTGD"
     gene            complement(513..1361)
                     /gene="cmr4"
                     /locus_tag="PLY05_11490"
     CDS             complement(513..1361)
                     /gene="cmr4"
                     /locus_tag="PLY05_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HNC03962.1"
                     /translation="MIQRTFLLHALSALHVGTGQSVGTVDLPIARAKASNLPLVPGSA
                     IKGVLRDELKIDTKDHKALFGPESTKAEVETHAGAIAVGDANLLILPIRSFAGTVAFA
                     TCPFILKHYARDMAIKDKIPVPAAETANVCTQTELKLANKVALEDLDLTANNNADTQT
                     WATKIATALYPTDTAWQAEFTKRFIILPDNIFSFLADTATEIRMRISIDRSTRIVKEG
                     ALWSEENLPAESVLWGVMGISQSRDKNHVKSAEDLSKLLPKGEHSLQIGGKHTVGRGL
                     CRMVLA"
     gene            complement(1394..>1686)
                     /locus_tag="PLY05_11495"
     CDS             complement(1394..>1686)
                     /locus_tag="PLY05_11495"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNC03963.1"
                     /translation="INNDLEGIPPFCPDLRLKLKAVAIERWLPVSGWDLAQWKPKAMR
                     KAVAAGAVYWFEVIGDIPNNLHEKLWLTAIADDQQDQRDGFGMILPAAWQTI"
BASE COUNT          467 a          345 c          338 g          536 t
ORIGIN      
        1 atcaggtgcg aatcacgctc agtgataatg acacttttct gttttttcgt tttgttccca
       61 aataggtaag ctgtggctaa gtaataggcc agcatgggca gattttgttt gatgcacggt
      121 ttcttttaaa gaagctctca ttaatgcaac catatcaatc tcctgttaat agggcttgtg
      181 tgtagcgttt aagccatgca gcagcctcta aggctttgcg tgtatgttgt tgataatcat
      241 tcagattgct gttaattatc gtttggtgca aattattagc attttgtagc ccaagaactt
      301 ctgctaagtc ttttaagtaa gcctgatgtt ctactttatt ttttgccaat aaaaagccga
      361 tagattgagt caagccactt tgtaaaatca tcacaggaaa gttaagagct agggttcgat
      421 aacctttttc agtatctttg cctctttgat aatcaacttt gggataagcc gctttggcat
      481 atttttggtc acgtgtttcc atgttgtact ccttacgcca aaaccatacg acacaaacca
      541 cgacctacgg tatgtttacc cccaatttgt aaagagtgtt caccttttgg aagtaacttt
      601 gacaagtctt cggcactttt aacatgattt ttatcacgtg attgactaat tcccataacc
      661 ccccacaata cactttcggc aggtaaattt tcttctgacc acaaggcacc ttctttaaca
      721 atgcgtgtgc tacggtcaat actaattcgc atacgaattt cggtggctgt atcagccaaa
      781 aatgaaaaga tgttatcggg taaaatgata aaacgtttag taaactctgc ttgccaagcg
      841 gtatcggttg gataaagagc agtggcaatt tttgttgccc acgtttgagt gtctgcattg
      901 ttgttggctg tgaggtctaa atcttctaag gcaactttgt tagctaactt aagttcagtt
      961 tgtgtacaaa cattagcagt ttcggcagca ggtacaggaa ttttatcttt aatagccatg
     1021 tcacgtgcat aatgctttag gataaacgga cacgttgcaa aagccactgt accagcaaac
     1081 gagcgaatcg gtaaaattaa tagatttgca tcacctaccg caatcgctcc tgcatgtgtt
     1141 tctacttctg ctttagtgct ttctgggcca aaaagagctt tatgatcttt ggtatcaatt
     1201 ttaagctcat ctcgtaatac tcctttaatc gctgagcctg gaactaaagg caaattagag
     1261 gccttggcac gagcaatagg taaatcaacg gtgccaacac tttgaccagt acccacatgt
     1321 agggcagaaa gggcatgcaa taaaaaagta cgttgtatca tgtgtgctta tccttgtatg
     1381 ttaggtattt atattaaata gtttgccaag cggcgggtaa aatcatgcca aaaccatctc
     1441 gctggtcttg ttggtcatcg gcaattgctg ttagccagag tttttcgtgt aagttattgg
     1501 gtatatctcc aatgacttca aaccaatata cagcacctgc tgcaactgct ttacgcatag
     1561 cttttggttt ccattgagcc aaatcccacc ccgaaacagg tagccaacgc tcaatagcaa
     1621 ctgctttgag tttgagtcgc aaatctggac aaaagggtgg aataccctct aaatcattat
     1681 taatcc
//
LOCUS       DAOQMB010000501         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53646_length_1685_cov_19.919018, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000501 DAOQMB010000000
VERSION     DAOQMB010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53646_length_1685_cov_19.919018"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="PLY05_11500"
     CDS             <1..609
                     /locus_tag="PLY05_11500"
                     /inference="COORDINATES: protein motif:HMM:NF020462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HopJ type III effector protein"
                     /protein_id="HNC03964.1"
                     /translation="QNEGSCRIFAFALLNNLNEQETLACFGRFYQEVLNDPQGSGHGN
                     IRNFMRDGWAGISFEGKALTAKRKHLAEGSTGTASMTVTDMDTAKAIAVMADDDFPEV
                     FATARMVGLMELAAARCLKVLLNDGELSVGVGVDIQHLAATPKYEVVQAVATYVGKEG
                     KLYKFTIEAFDAGGKIGEGTHTRAIISTERLVAGAKARVALN"
     gene            711..>1685
                     /locus_tag="PLY05_11505"
     CDS             711..>1685
                     /locus_tag="PLY05_11505"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HNC03965.1"
                     /translation="MTQQRPYIDTHQEQDDTNHSVNSHFNEVLQVRLSRRKTVLGGMS
                     ATAALMLGGLTGCGNSDDTVVPAATPEPELKLGFTPVAKNLNDVVTVPTGYEVAVVYA
                     LGDALNTTTSAWANDGSETADSYEFRAGDHHDGMSFFGLGSDGKWSSKTSDKGLLCLN
                     HENITSAFLHASGPTTVDGKRPVDEALKEINCHGVSVVAINRQSNGSFAVDKANTLNR
                     RVTPNTVMEFHGPAAASRFLVTKYDAKAKKGRGTLNNCANGYTPWGTYLTCEENWAGY
                     FKRPSTDDSLRSTAELVSLKRYGVTSSSGANSWSTASDSDDRFTRWNAT"
BASE COUNT          488 a          310 c          385 g          502 t
ORIGIN      
        1 caaaatgaag gctcttgccg tatttttgcc tttgctttat taaataattt aaatgaacaa
       61 gaaaccttag cctgttttgg tcgtttttat caagaagtct taaatgatcc ccaaggctca
      121 ggtcatggca atattcgtaa ttttatgcgt gatggttggg caggcattag ttttgagggt
      181 aaagcattaa cagccaagcg caaacattta gccgaaggta gcactggcac tgcctcaatg
      241 acagtgaccg atatggatac tgctaaagcg attgcggtga tggcggatga tgattttcct
      301 gaagtatttg ccaccgcaag aatggttggt ctaatggagt tagccgctgc acgttgttta
      361 aaagtgctgt tgaatgatgg tgaattatcc gttggggttg gagttgatat tcagcatcta
      421 gcagcaacgc ccaaatatga agttgttcaa gcagttgcta cttatgtagg caaagaaggc
      481 aaactctata aatttactat tgaagctttt gatgcaggtg gcaaaattgg cgaaggcaca
      541 catactcgtg cgattattag caccgaacgt ttagtggctg gggcaaaggc tagagttgca
      601 ctcaattaaa aacggttttc actaaactgt catcgtttag tcaaatcaat gtcatcagtg
      661 gtacttagtg tatagcgcag accatttccg ctatacatta ggtatataac atgacacaac
      721 aacgccctta tattgatact catcaagaac aagacgacac caatcattca gttaattctc
      781 attttaatga agtcttacaa gttcgtttat ctcgccgtaa aaccgtttta ggtggcatgt
      841 cagcaactgc tgctttaatg ttaggtggtt taactggctg tggtaattcc gatgatacag
      901 tcgttcccgc tgcaacacct gaaccagaat taaaattagg tttcacacca gttgctaaaa
      961 atttaaatga tgtggttact gtaccaacgg gttatgaagt cgcagttgtt tatgctttag
     1021 gggatgcgtt aaacacaaca acttctgcat gggcaaatga tggctctgaa acagcagatt
     1081 cttatgagtt tcgtgcaggc gaccatcatg atggtatgag cttttttggt ttaggcagtg
     1141 atggtaaatg gagttcaaaa acctcggata aaggtttgtt gtgtttaaat catgaaaata
     1201 ttaccagtgc ttttttacac gctagtggtc caacaacagt cgatggcaaa cgtcctgttg
     1261 atgaagcctt aaaagaaatt aattgtcatg gcgtcagcgt tgtcgcgatt aatcgtcaaa
     1321 gcaatggttc atttgcggtt gataaagcga ataccttaaa ccgtcgtgtt acaccaaaca
     1381 cagtgatgga gtttcacggc cccgctgcgg ctagtcgttt cttggtgact aaatacgatg
     1441 ccaaagccaa aaaaggacgc ggtacattaa ataattgtgc taacggttat acgccttggg
     1501 gtacttattt aacttgtgaa gaaaattggg caggttattt taaacgccca agtacggatg
     1561 atagtttacg tagcacagct gaattagtta gcttaaaacg ttatggtgtt actagctctt
     1621 caggtgcaaa ttcatggtca acagcaagtg atagcgatga ccgttttacc cgttggaatg
     1681 ccact
//
LOCUS       DAOQMB010000502         1685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53649_length_1685_cov_15.265644, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000502 DAOQMB010000000
VERSION     DAOQMB010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53649_length_1685_cov_15.265644"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..1613)
                     /gene="ccoN"
                     /locus_tag="PLY05_11510"
     CDS             complement(186..1613)
                     /gene="ccoN"
                     /locus_tag="PLY05_11510"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011513131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HNC03966.1"
                     /translation="MSHSHTSNAPIAYEMGVVRQFAIMTVIWGIVGMAVGVFIAAQLV
                     WPALNFDTPWLTYSRLRPLHTNAVIFAFGGSALFATSYYIVQRTCQTALFMPSLAKFT
                     FWGWQLVIVSAAISLPLGLTSSKEYAELEWPIDLLIAVVWVAYAVVFFGTIAKRTTSH
                     IYVANWFLGAFIITIAVLHIINSMAVPVSLTKSYSMYSGATDAMIQWWYGHNAVGFFL
                     TAAFLGMMYYFIPVQVGRPVYSYRLSVVHFWALISVYMWAGGHHLHYTALPDWTQSLA
                     MAFSLVLFAPSWGGMINGIMTLSGAWDRLRTDPIIRFLVVALSFYGMSTFEGPMMSIK
                     TVNALSHYTDWTVGHVHSGALGWVGMISIGSLYVLIPRVFDRERMYSISLINLHFWLA
                     TIGVVIYIASLWISGITQGLMWRAVNEDGSLTYSFVEAVVASHPYWFGRFIGGALYLT
                     GMLVMAYNVAMTVMNKKPVGADKLG"
BASE COUNT          583 a          374 c          329 g          399 t
ORIGIN      
        1 tctaagcgag catcagcttt aggatcattc aattcttgtt ggaagaataa aggaacaatc
       61 tctaccaagg ttgcaaaact aattgcaacg caaattaaca caattaatgc gcctacattt
      121 ttctcgacga gatcatgcac attattgctt ggggcattgt gatcagccat ttgaattcct
      181 cccgcttagc ctaatttgtc tgcgccaaca ggttttttat tcatgacggt catggcaacg
      241 ttataagcca ttaccaacat acctgttaaa tataaagcac caccaatgaa gcgaccaaac
      301 cagtaaggat ggctagcaac aacagcttca acaaagctat aagttaaaga accgtcttcg
      361 ttaacagcgc gccacattaa gccttgggta ataccagaaa tccacaatga agcaatgtaa
      421 attactacac caatagtagc taaccagaag tgtaagttaa ttaaactaat gctatacata
      481 cgctcacgat caaaaacacg tgggattaac acgtataaag agccgatact gatcataccc
      541 acccaaccta aagcacctga gtgtacgtga ccaacagtcc agtctgtgta gtgtgataaa
      601 gcattaacgg ttttgataga catcattggg ccttcgaagg tggacatacc atagaaggat
      661 aaagccacaa ctaagaagcg gataattggg tcagtacgca aacgatccca tgcaccagat
      721 aaagtcataa taccgttaat catgccaccc cagcttggtg caaataacac taaggagaag
      781 gccattgcca aagattgagt ccaatctggt aatgcggtgt agtgtaaatg gtgaccacca
      841 gcccacatat aaacagaaat taatgcccag aagtgaacaa cagacaaacg gtaagaataa
      901 actggacgac ctacttgtac agggatgaag taatacatca tgcccaagaa agcagccgtt
      961 aagaagaaac ctactgcatt atggccatac caccattgaa tcatcgcatc agtcgcacca
     1021 ctatacatag agtaagactt ggtcaagctt acaggcacag ccatgctatt gataatatgt
     1081 aatacggcaa tagtaataat aaacgcacct aagaaccagt tagctacata gatgtgggat
     1141 gttgtacgtt tagcaattgt gccaaagaaa acaacagcat aagcaaccca caccacagca
     1201 attaacaagt caatcggcca ctctaattct gcatattctt tagaagaagt taagcctaag
     1261 ggtaaactaa ttgctgctga cacaatcact aattgccaac cccaaaaggt aaatttagca
     1321 agactaggca tgaataatgc tgtttgacag gttctttgca caatatagta cgaggtggcg
     1381 aataaagcgg atccgccaaa agcaaaaata accgcgttcg tgtgtaaagg acgtaaacgg
     1441 ctataggtta accaaggtgt atcaaaatta agagcgggcc aaactaactg ggcggcaata
     1501 aatacgccca cggccattcc aacaattccc caaatcacgg tcatgatggc aaattgtcga
     1561 actactccca tttcgtacgc tattggtgcg ttactggtgt ggctgtgact catgcattgc
     1621 tccttaaacc acaaggttaa taggggaatt attctgattt aatgcctcaa agttacttcc
     1681 ttgcg
//
LOCUS       DAOQMB010000503         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53870_length_1682_cov_12.748002, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000503 DAOQMB010000000
VERSION     DAOQMB010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53870_length_1682_cov_12.748002"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..914
                     /gene="cysE"
                     /locus_tag="PLY05_11515"
                     /pseudo
     CDS             228..914
                     /gene="cysE"
                     /locus_tag="PLY05_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
     gene            1170..1646
                     /gene="iscR"
                     /locus_tag="PLY05_11520"
     CDS             1170..1646
                     /gene="iscR"
                     /locus_tag="PLY05_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009088710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HNC03967.1"
                     /translation="MRLTTKGRYAVTAMLDLAIHARLEPVSLADISERQSISISYLEQ
                     LFAKLRKGGLVSSVRGPGGGYQLARHGSEIFVAQIIDAVDELVDATRCAGQGDCQQGM
                     MCLTHDLWTDLSARIHSFLSNISLEELVHRRGVQAVALRQSKVLHPEGHDLGLLLM"
BASE COUNT          460 a          311 c          399 g          512 t
ORIGIN      
        1 cgtgtacggc gaatgtttag ccgtttgcgt ttagatcgac tagagtataa cttatggcga
       61 ggtgttttta gtcgtatgca ggcaatggca gccgaaatta caaagcttaa aaaagagtaa
      121 aagtgtatga gtcatgttta taacaatctg tttgttttat taaccacacg atgttgttgc
      181 taaaagtatt tttggtagtc aacaatcatc cttaagaggt tataaacatg ttttcattag
      241 ttaagcagtt acacgaagat gttcagtcgg tatttgcccg tgatcctgcg gcacgtaata
      301 ccttagaagt gattatcaat taccctggta ttcatgccat tgccttgcat cgtgtcgctc
      361 atgggttatg gcaatctgaa tataaaggca cagcaagagc ggtctccaca tttagccgtt
      421 ttttaacagg catcgaaatt catcctgctg ctaaaattgg ccgtcgcttt tttattgatc
      481 atggtatggg cgtagtcatt ggtgaaaccg cgcaaattgg tgatgatgtg accttatatc
      541 atggtgtgac tttgggtggc acaacatggc aaaaaggcaa gcgtcatccc actttagaag
      601 atggtgttgt cgtaggcgca ggtgcaaagg ttttagggcc ttttacggtg ggtaaaggtg
      661 caaaaattgg ttctaatgcc gttgtgacca aagctgttcc cgcaggcgca accgcggtgg
      721 gtaatcctgc acgtttaatt ttaaaacaag tattagaagt gcatcctgat gaacaagctc
      781 gtttggattt tgcccaaaaa ataggttttc aagcctatgc agccacgccc gatttacctg
      841 atcctgtggt tgaagcgtta cgcgtcttat tagaccacat gcaagcaacc gataaacgtt
      901 tagacaaaat gtgtggcgca ttaaaacgcg tcaacaaaga cttttgtgat gaacggccaa
      961 gcgaattaaa agctgaagat ttagtggtga tgcaagaggg tggtagttaa aaaattgacc
     1021 ataagccaag tagggttaca ctattataaa cttgactaaa ttactcggaa ttagctatac
     1081 ttctcatgaa tacagactta cgcaccttat tcgtttaagt gtgttgttat ttgttcggcg
     1141 taagtcttgc gtatacagga gaaatcacga tgcgcttaac aaccaaaggc cgttatgctg
     1201 ttactgcaat gctcgatttg gccattcatg ctcgtttaga gccagtcagt ttggccgata
     1261 tttctgaacg ccaatctatt tcgatttctt atttagagca gttgtttgcc aagttacgca
     1321 aaggcggttt ggtgtctagc gtccgtgggc caggcggtgg ttatcaatta gcacgtcatg
     1381 gttctgaaat ttttgtcgca caaatcattg atgcagttga tgaattagtc gatgcaacac
     1441 gttgtgcagg ccaaggcgat tgtcaacaag gtatgatgtg tttaacacat gatttgtgga
     1501 ctgatctgag tgcaagaatt catagttttt tatccaatat tagtttagaa gagctggtac
     1561 atcgtcgtgg tgtgcaagcc gttgctttac gtcaaagcaa agtattacat cctgaggggc
     1621 atgatctcgg tctgttgctc atgtaatccc agcaatcttt aacctaacat gaagtagggt
     1681 ag
//
LOCUS       DAOQMB010000504         1681 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53920_length_1681_cov_13.026445, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000504 DAOQMB010000000
VERSION     DAOQMB010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53920_length_1681_cov_13.026445"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            583..843
                     /locus_tag="PLY05_11525"
     CDS             583..843
                     /locus_tag="PLY05_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HNC03968.1"
                     /translation="MPLASRHQKTLADIFHRPTSATIKFSDIEKLVVALGGVIENNDG
                     SRMKFCLNGHAMAAHRPHPNKEAKRYQVEDMREFLQKQGITP"
     gene            849..1193
                     /locus_tag="PLY05_11530"
     CDS             849..1193
                     /locus_tag="PLY05_11530"
                     /inference="COORDINATES: protein motif:HMM:NF017355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HNC03969.1"
                     /translation="MSYKGYTTSIWFDDRDEIFVGRVLGISDIVSFHADNVSELKKEF
                     HSAIDAYLEPSLALGRPPQKPFSGKLSLRLPSELHKNVAQLAQEKQASINELIVKAVQ
                     ELLVHERSVIGH"
     gene            1408..>1681
                     /locus_tag="PLY05_11535"
     CDS             1408..>1681
                     /locus_tag="PLY05_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNC03970.1"
                     /translation="MFTISETPLFTRLWPDYWTEDERGKFAAFLATHPEAGDVVQGSG
                     GVRKVRWHKAGSGKSGGVRVIYYNQLADGHIWLLTIYSKNVRDTLDA"
BASE COUNT          507 a          273 c          344 g          557 t
ORIGIN      
        1 acaaatctga atgatgacga agaaaaacta cgattctctg actagctgtg gtttaataca
       61 ttatccattt cctttaaaga tgtgataagc agaattgact ttgatgaacg tcaagaattt
      121 tatcttgatg tcaatagcgt cagctttata gcagataaat ttaataaatt tagtgatgat
      181 gagtttaggc ttagatttaa ttctactgag tttaaagcag agttagagca aaataaattt
      241 atgagagata tattttctca tttaaatttt ggtaatgagt cttattttca gggtttacta
      301 cattcattta gaaagttgtc agaatttctt ataaatatgg aaaagaaaaa atatgtgata
      361 attttcttcg atgtctaact tgcgcgtcaa gtgcgacaag tacccagccg attattgtgt
      421 tattttttat ggttctgagg taatcttagt ttttgatttt gttgtgggta cttgcgcgtt
      481 atgcgcctag ttagactttc gtggctttga tagaaaaaag tgtattgcta attcttgaaa
      541 atacactaca atggcttttt gataccaatc aatgatacca ttatgccgtt agccagtaga
      601 catcaaaaaa cgcttgccga tatttttcat cgcccaacat cagccactat taaattttct
      661 gacattgaaa aactggtagt tgcattaggt ggagtgattg aaaataatga tggctctcgt
      721 atgaagtttt gtctaaatgg tcacgcaatg gcagcacata ggccgcatcc aaataaagag
      781 gctaaacggt atcaagtgga agatatgcgc gaattcttgc aaaagcaggg tattacacca
      841 tgaacactat gagctataaa ggttatacta catcaatttg gtttgacgac cgtgatgaaa
      901 tttttgtagg ccgcgtactt ggcatttcag atattgtcag ttttcatgct gataatgttt
      961 ctgagttaaa aaaagagttt cactcggcaa tagatgctta tcttgagcca tcgttagcct
     1021 tgggaagacc tccacaaaaa ccattctcag gtaaactttc attgagattg cctagtgagt
     1081 tacataaaaa tgttgctcaa cttgcccaag aaaaacaagc aagcatcaat gaacttattg
     1141 taaaagctgt gcaagagttg cttgtacatg agcgttcagt tatcgggcat taggcttttg
     1201 ccttacctac cagtctaact tacgcgtcaa gtgtgacaag cacccagccg attacggagc
     1261 tattttttga gctcagtgat tactgagttt ttgtttattt cgttgtgggt gcttgcgcct
     1321 tacgcggata gttagccatt ttataaacta tttgctatta gccgtcattg acgtataatt
     1381 agctaataag catttagtag tatttgtatg tttaccattt cagaaacacc acttttcaca
     1441 cgtttatggc cagattattg gactgaagat gagcgtggaa aatttgcagc gttcttagca
     1501 acacatcccg aagcaggaga tgttgttcaa ggctcaggtg gtgttcgtaa agtgcgttgg
     1561 cataaagcgg gtagtggtaa atcaggtgga gtacgtgtca tttactataa tcaacttgct
     1621 gatggccata tttggctatt aaccatttat agtaaaaatg ttagagatac tttagatgct
     1681 a
//
LOCUS       DAOQMB010000505         1680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_53988_length_1680_cov_15.088000, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000505 DAOQMB010000000
VERSION     DAOQMB010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53988_length_1680_cov_15.088000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="PLY05_11540"
     CDS             <1..1638
                     /locus_tag="PLY05_11540"
                     /inference="COORDINATES: protein motif:HMM:NF024757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HNC03971.1"
                     /translation="ASKDYAMILVKDLPLSNGAGKVAWTSSELLTSRSYNGDALVIVH
                     GVANNQGELVLEGLGNATVITRDNGINIAEQNANRLALTYSHYPHLELVVRSEAGKTV
                     RVLVTTTEEAGRFWFPRINNQDYVVAGLELLKETGVSSVYNLQLAAEYDVKNRPLFVM
                     SPRRMQLRNLVVNSAWNSQTQSATFTKPQSIPSLPALPSLMVGKVASDMAEAKLDYND
                     STWIKWTGEPQPLETKGIYTGHAWYRTELDFDHAPYWWEDCSLYIEHASDIVGIYVNG
                     TYLSTKLPVGTELDSDSWNDKYKFPSLRPYLKQGKNVVAFRVESWGHGSFMFPRGTLA
                     ATNMKIPSLGFDSVKGLWGTARLGGTNLTNWSLRADLNGERTGLMSNTYSDSTWQSAT
                     LPMNLAKGEVRWYRAKFDASQLPNTSNLHAPLSLRFAGQRVKATVWLNGQLIGRWLSG
                     DGWIDNGFWGRAIRDMWMNTNPDYFPISTTGLNPAGTANVLAVAFEDVSDGGESAGQV
                     GEVSLAYSPEIRADINGQSMLVNQPRGKTLLNVDFLE"
BASE COUNT          482 a          302 c          406 g          490 t
ORIGIN      
        1 gcttctaaag actatgcgat gattttggtc aaagatttac cgttaagcaa tggtgcaggc
       61 aaagttgcat ggacaagcag tgagttatta acgtcacgca gttataacgg tgatgcttta
      121 gtgattgtgc atggtgtggc caataatcaa ggtgaattgg tgttagaagg tttgggcaat
      181 gctaccgtta tcacccgtga taatggtatt aatattgccg aacaaaatgc caatcgttta
      241 gccttaacct atagccacta tccacattta gaattagtgg tgcgtagtga agctggtaaa
      301 acggtgcgtg tattagtgac aactaccgaa gaagcaggac gtttttggtt tccacgtatc
      361 aataatcaag attatgtggt ggcaggttta gaattgttaa aagaaacagg tgtatcctca
      421 gtatacaact tacagttggc agcggaatat gacgttaaaa atcgtccctt gtttgttatg
      481 tcaccacgtc ggatgcagtt gcgtaattta gtggtgaaca gtgcatggaa tagtcaaacc
      541 caaagtgcaa cttttaccaa accacaaagt atccccagtc tgcctgcatt acccagtttg
      601 atggtaggta aagtagccag cgatatggcc gaagccaaat tagattataa cgacagtaca
      661 tggataaagt ggacaggtga gccacaacct ttagaaacaa aaggtattta tacaggtcat
      721 gcttggtatc gcaccgaatt agattttgac catgcgccat attggtggga agattgttct
      781 ttatatattg aacatgccag tgatattgtt gggatttatg tcaacggcac gtatttaagc
      841 actaagttac ctgtaggcac tgaattggat tcggattctt ggaatgataa atataaattc
      901 ccgtcattac gtccttactt aaaacaaggc aaaaatgtgg tggctttccg tgttgaatca
      961 tggggacacg gtagctttat gttcccacgt ggcacattag ctgcaaccaa catgaaaatt
     1021 cccagtttgg gttttgatag tgtcaaaggt ttgtggggaa cagcgcgttt aggtggcact
     1081 aatttaacga attggtcgtt acgtgccgac ttaaatggtg aacgtactgg tttaatgagt
     1141 aacacttata gtgatagcac atggcaatct gcaactttac ccatgaactt agccaaaggc
     1201 gaggtgcgtt ggtatcgtgc caagtttgat gctagccaat tacccaatac cagcaattta
     1261 cacgcgcctt tatccttacg ttttgctggc caacgtgtta aagcaaccgt ttggttaaat
     1321 ggtcagttaa ttggtcgttg gttaagtggt gatggttgga ttgataacgg attttggggg
     1381 cgtgcaattc gtgatatgtg gatgaatact aacccagatt atttccccat tagcacgaca
     1441 ggcttaaacc cagcaggtac cgccaatgtg ttagctgttg cctttgaaga tgtgtctgat
     1501 ggtggtgaaa gcgcaggaca agtaggtgaa gtatctttgg cctatagccc agaaatcaga
     1561 gccgatatta atggtcagtc gatgttggtc aatcaaccac gcggtaaaac gctgttgaat
     1621 gtggactttt tagagtaatt tacgctaact aaaaaaggct tgggaaacca agcctttttt
//
LOCUS       DAOQMB010000506         1676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54224_length_1676_cov_9.880938, whole genome shotgun sequence.
ACCESSION   DAOQMB010000506 DAOQMB010000000
VERSION     DAOQMB010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54224_length_1676_cov_9.880938"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLY05_11545"
     CDS             complement(<1..781)
                     /locus_tag="PLY05_11545"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNC03972.1"
                     /translation="MRKSLLASAITLLTCQTYATADDFLFAEQEMPVVLSATRLKQAV
                     ADAPASVTIIDRQMIAQSGAREIPDMLRLVPGMVVGYEKGWDAFVSYHGTSADMARRM
                     QVLVDGRSVFQPSLAFVDWIGLPLELDDIDRIEVIRGPNAAAYGANSFLAIVNIITRN
                     PADLPTVRAYTRQGGEGINDNFVSYAGVKNQFSWQLSANQRNDDGFSTYYQKETQRDA
                     QGNKVFDQNGDPVKVFNPYPYADDKRQKAIYGQGILEGNTYF"
     gene            complement(829..>1676)
                     /gene="der"
                     /locus_tag="PLY05_11550"
     CDS             complement(829..>1676)
                     /gene="der"
                     /locus_tag="PLY05_11550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNC03973.1"
                     /translation="PNVGKSTLVNRLLGEDRVIVYDMPGTTRDSIYIPYERNGKKYTL
                     IDTAGVRRRGRVHETVEKFSVIKTLQAIKDAHVVIIVFDAREGIVEQDLHMLGFALES
                     GRAVVLAINKWDNMTEYDRNQVKLAIERRLDFVPFVKIHLISALHGTGVGDLYPSIQR
                     AYDSAMIKVPTNRLTQILQDAVASHQPPLVNGRRIKLRYAHMGGQNPPIIVIHGNQTD
                     AVSKDYKRYLENIFRKVLKLQGTPVHLEFKTGDNPYKEEKVLKPAQLEKRRYSAVEKK
                     TKEKR"
BASE COUNT          489 a          382 c          296 g          509 t
ORIGIN      
        1 cgaagtaagt atttccctct aaaatccctt gaccataaat ggctttttgg cgtttatcat
       61 cagcataggg ataaggatta aaaactttga caggatcacc attctggtca aatactttat
      121 taccttgggc atcgcgttga gtttcttttt gataataggt actaaaacca tcatcattac
      181 gctggttagc cgacaactgc caactaaatt gattttttac gcctgcataa ctgacaaaat
      241 tatcattaat gccttcgcca ccttgacgtg tataagctcg taccgtaggc aaatcggcag
      301 gatttcgggt gataatattc acaattgcca aaaaactatt tgccccatac gcagcagcat
      361 ttggccctct aataacttca atccggtcaa tatcgtctaa ttctaacggc aacccaatcc
      421 aatcgacaaa cgctaaactc ggttgaaaca ccgaacgacc atcaaccaat acttgcatcc
      481 gccgcgccat atctgccgat gtaccatgat aactgacaaa agcatcccaa cccttttcgt
      541 aaccgaccac cataccaggt actagacgca acatatcagg aatttctcgt gcacccgatt
      601 gggcaatcat ttgtcggtcg atgatagtga ctgaggcagg cgcatcagca accgcttgtt
      661 ttaaacgtgt tgccgataac acgacgggca tttcttgctc tgcaaacaaa aaatcatcgg
      721 cagttgcata ggtttgacaa gttaataagg taatcgccga agctaataac gattttctca
      781 taaaaacccc agttatcact aacaggggct tttctgttat ataccctatt agcgtttttc
      841 ttttgttttc ttttctactg ctgagtagcg tcttttttca agttgtgcag gttttaacac
      901 tttttcttcc ttatacggat tatcgcccgt tttaaactct agatgtactg gtgttccttg
      961 tagttttaat accttcctaa aaatattttc taaatagcgt ttataatcct tagatactgc
     1021 atcagtttga ttaccatgaa tcacaataat cggaggattt tgtcctccca tatgtgcata
     1081 acgcaattta atacgacgac cattaaccaa tggcggctga tgacttgcta ctgcatcttg
     1141 taaaatttgc gttaagcggt tggtagggac tttaatcatt gccgaatcat aggcacgttg
     1201 aatcgacgga tataaatcac ctacacctgt accatgtaaa gccgatatta aatgaatttt
     1261 gacaaaaggt acaaaatcta aacgtcgttc aatggctaac tttacttgat tacgatcgta
     1321 ttcagtcatg ttatcccatt tattaatagc taacaccact gcacggccac tttctaaagc
     1381 aaatcctagc atgtgtaagt cttgctcgac aataccttcg cgtgcatcaa aaacaataat
     1441 gacgacatgg gcatctttaa tggcttgtag ggttttaata accgaaaact tttcgactgt
     1501 ttcatggact cgaccgcgac gacgaacgcc tgcggtatca attaaggtgt attttttgcc
     1561 gtttcgctca taaggaatat aaatactatc gcgcgttgta cctggcatgt cataaacaat
     1621 aaccctgtcc tcgcctaata aacgattgac taaggttgac ttacctacat taggac
//
LOCUS       DAOQMB010000507         1674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54357_length_1674_cov_16.240889, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000507 DAOQMB010000000
VERSION     DAOQMB010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54357_length_1674_cov_16.240889"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLY05_11555"
     CDS             <1..812
                     /locus_tag="PLY05_11555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC03974.1"
                     /translation="HLTQPAISKRVQNLEETLAVNLFDRVGRKVQLTEAGRNLLPRAK
                     QWLLDFEEIRNSVTKLQGDVAGILKIGTSHHIGLHRLPPTLRAFSQAYPQVRLDIQFI
                     DSEAAYESVLSGDLELGIVTLPPVADPRLLTEKIWDDPLAFVAGKDHPLANKANIQLA
                     DLTGHPAILPSLNTFTRQIAAELFKSSGLELNVTMTTNYLETIRMMVSIGLGWSVLPA
                     SMANEEMVVLPLAEAAKMHRDLGVVYHPRRTLSRAASAMLAVLRERNELKL"
     gene            865..1674
                     /locus_tag="PLY05_11560"
     CDS             865..1674
                     /locus_tag="PLY05_11560"
                     /inference="COORDINATES: protein motif:HMM:NF021361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HNC03975.1"
                     /translation="MIAKFCIVLSFLTLAACQTAPQQTTVIVPPPPHANINQLLTVSQ
                     FRTPLFYLEAPLYDPEVKEWIPQKFLDDVQKSIDDTINYPYTDTQLRLALSQKLSSTE
                     TQQVIDFYQSTTGQNILQAEQSFRERVNLSSDSTALAADVIKATLLSTMLHNVFMSSV
                     DAVISRLDSYECLAIRQIPGSNIGLLMAKRHKVAFMERQVQRSLSNLYLNVSDADLQA
                     YLQFAQSAVGQKYFKARNEAMLSIGKEFGERVAEAIAPALPNCMGSFKVSS"
BASE COUNT          457 a          312 c          389 g          516 t
ORIGIN      
        1 tacatttaac ccaacctgcg attagtaaac gggtacaaaa tttggaagaa accttagcgg
       61 tcaatttgtt tgaccgtgtg ggtcgcaaag tgcaactgac cgaagcgggt cgaaatttgt
      121 tgcctcgtgc caaacaatgg ttactggatt ttgaagaaat tagaaacagt gtgactaaac
      181 tgcaaggtga tgtggcaggt attttaaaaa ttggtactag tcatcatatt ggtttacacc
      241 gtttgccacc tactttgcgt gctttttccc aagcttatcc gcaggtgcgt ttagatattc
      301 aatttattga ttccgaagcg gcttatgaat cggtgttaag tggtgattta gaactgggta
      361 ttgttaccct gccgcctgtg gctgatccac gcttgttaac cgaaaaaatt tgggacgatc
      421 ctttggcttt tgttgcaggt aaagaccatc ctttggccaa caaagccaat attcaattgg
      481 cggatttaac agggcatcct gccattttgc cgtcattaaa cacttttacc cgacaaattg
      541 ctgctgagct gtttaaaagt tctggtttag aattaaatgt caccatgacc accaattatt
      601 tagaaacgat tcgaatgatg gtgtcgattg gtttgggttg gagtgtgttg cctgccagca
      661 tggccaatga agaaatggtg gtattgcctt tagccgaagc agcaaaaatg catcgtgatt
      721 tgggcgtggt ttatcatccc cgtcgaacct tgtctcgtgc tgcaagtgct atgttagcgg
      781 tgttgcggga gcgaaatgag ttgaagcttt aagcggatat gtttactctg tttggcaatt
      841 tataactaca acgcgtatta ccctatgatt gccaaatttt gcattgtgtt aagcttttta
      901 accttagcgg cttgccaaac tgccccgcaa caaacaaccg ttattgttcc accgcctccc
      961 catgccaata tcaatcaact gttaacggtt agccaatttc gtacaccgct tttttattta
     1021 gaagcaccac tgtatgaccc cgaagttaaa gaatggattc cacaaaaatt tttagatgat
     1081 gtgcaaaaaa gcattgatga cacgattaac tatccttata ccgacacaca gttacgttta
     1141 gctctgtcgc aaaaattaag cagtacagaa acacagcaag tgattgattt ttatcaatca
     1201 acaacaggcc aaaacatttt acaagcggag caaagtttta gagagcgggt taatttaagc
     1261 tctgatagca cggctttagc agctgatgtt attaaagcta cgttgttatc aaccatgctg
     1321 cataatgtgt ttatgagcag tgttgatgcg gtgattagtc gtttagatag ttatgagtgt
     1381 ttggcgattc ggcaaattcc cggttctaat attggcttgt taatggcaaa acgtcataaa
     1441 gtggctttta tggagcggca ggtgcaacgc tcattgagta atttatattt gaatgtgagt
     1501 gatgccgatt tgcaagcgta tttgcaattt gcccaaagtg cggttggaca aaaatatttt
     1561 aaggcgcgta atgaggcgat gttgagtatt ggtaaggagt ttggtgaacg tgtggcagag
     1621 gcaattgccc ctgctttgcc aaattgtatg ggtagtttta aggtgagttc gtag
//
LOCUS       DAOQMB010000508         1672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54488_length_1672_cov_6.559678, whole genome shotgun sequence.
ACCESSION   DAOQMB010000508 DAOQMB010000000
VERSION     DAOQMB010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54488_length_1672_cov_6.559678"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..482)
                     /locus_tag="PLY05_11565"
     CDS             complement(291..482)
                     /locus_tag="PLY05_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03976.1"
                     /translation="MNESNAEVLVMAKENLLKTYQALPKEIQEQHPEIPQKIQEMKKL
                     SEVGKPLQAVKQYLPPLKK"
     gene            complement(833..1255)
                     /locus_tag="PLY05_11570"
     CDS             complement(833..1255)
                     /locus_tag="PLY05_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC03977.1"
                     /translation="MPYVLDTNIISELMKDNPNPKVIHWLDQQIISELFISSITVAEI
                     LFGIKRLEQGKRKSRLVQQAALILETHFGDRTYTFDKKSAVFYADIRQQRTKQGLPIS
                     HADCQIAAITCQYACILVTRNTKDFYGIDGLTLLNPFD"
     gene            complement(1255..1476)
                     /locus_tag="PLY05_11575"
     CDS             complement(1255..1476)
                     /locus_tag="PLY05_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03978.1"
                     /translation="MNTVLEQRVITLIHHLSDEQLNQVIHFIEQQEQQAKPQQGLGTL
                     LHAQFKAVGLTSVDFTPPERPIDEERVTF"
BASE COUNT          476 a          362 c          287 g          547 t
ORIGIN      
        1 ccttagcaaa ctgaaacatc actacctaca gccaccacta cggcttacgc cacggggcaa
       61 ggcagtggcc aattcctttg gtatataaga ctattatcaa gtggacgaaa catctacacc
      121 ctaagtctta tatttactca atttccgctg acgattctat cgcatagact tgcaacaatc
      181 aaatagataa aagtaagcct taattaagtt tctaacactg aaaggcttac tacactcttc
      241 ttcacgtaca gtatcagtaa cattttgaag tacagttact ctaccttcct ctacttcttc
      301 agtggtggta ggtactgctt cactgcttgt agtggttttc ctacttccga tagtttcttc
      361 atttcctgta tcttctgcgg aatttcggga tgttgttcct gaatttcctt tggcagtgct
      421 tgatacgtct ttagaagatt ctctttggcc attaccagta cttctgcgtt gctctcgttc
      481 atttgatttt tcctcgttat atacctatcg ccattgaaag tactggaaaa agctgttgaa
      541 attagtcatt actatacgtc ataaaatttg aacttatcag ccagccagca agcgtgggtt
      601 gacgcagaaa acccacaatt cgcgtttaat tatcgtgggt ttcgttcctc aacccacact
      661 acatccgcta taacctagcc caactccccg ccattttttt agcaatcata tcaattgctt
      721 gggcagcgac tggaattttt cctaattgca aaaagccatg aattaaatca tcaaaacaat
      781 gataagtgac aagcctatat gcagcatagc caaatacaaa cgttttggac tgttaatcaa
      841 atggatttaa aagcgttaag ccatcaatac cataaaaatc cttggtattt cgtgtaacta
      901 atatacaagc gtattggcac gttattgccg caatttggca atctgcgtga ctaatcggca
      961 atccctgttt agtgcgttgc tgtcgaatat cggcataaaa cactgcgctt tttttatcaa
     1021 aagtataggt tctatcacca aaatgagtct ctaaaattaa cgctgcttgt tgtactaatc
     1081 ggctcttacg tttgccttgc tctaagcgtt taataccaaa caaaatctct gcaaccgtaa
     1141 tagagctaat aaataactca cttattatct gttgatctaa ccaatgaata acttttggat
     1201 ttggattgtc cttcatcaat tcagaaataa tattggtatc taaaacataa ggcatcaaaa
     1261 agtcactctt tcttcatcaa tagggcgttc tggcggtgta aaatcaacac ttgttagccc
     1321 tactgcttta aattgggcgt gcaacaatgt gcctaagccc tgttgcggtt tggcttgttg
     1381 ttcttgctgt tctataaagt gaatgacttg attaagctgt tcatcgctta gatgatgaat
     1441 cagtgttatg acgcgttgtt ctaatacagt gttcattaca atactcctca tagcattttg
     1501 agtatatcgt tggtttggca attttagcat cctagcctgt atgcagcgtg caaaatacag
     1561 gagttttaat aaatagcatt attcctgtgt tacgttgcac ttcacacagg cgatcaatat
     1621 cataaactca cttcgcactc ttatacataa aattctctaa cgaggtttcg gc
//
LOCUS       DAOQMB010000509         1670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54636_length_1670_cov_14.998142, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000509 DAOQMB010000000
VERSION     DAOQMB010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54636_length_1670_cov_14.998142"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1587)
                     /locus_tag="PLY05_11580"
     CDS             complement(145..1587)
                     /locus_tag="PLY05_11580"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC03979.1"
                     /translation="MAEKFDVLIIGAGLSGIGAACHLKRECPNKTFVILEGRDAMGGT
                     WDLFRYPGIRSDSDMYTLGYNFKPWTNPKAIADGPSIRAYIHEAAQEYDVNRHIRYGH
                     KVLKAAWSTPKAAWTIEATNKAGEPVTIEANFVISCTGYYNYEAGYTPEFKGRERFKG
                     QIIHPQLWPENLDYTNKKVVIIGSGATAVTLLPSMTDKAAHVTMLQRSPSYVISVPQE
                     DIISNKLRKFLPEKVVYHMARVRNVGLSLGLYRYSRAKPAVVRRFLLSQVRRQLGQDF
                     DMSHFTPQYNPWDERLCAVPNGDLFKVLRKGKASVVTDHIDSFTEDGILLKSGKTLEA
                     DIIITATGLDIKLLGGIQLEKDGQAFDVTKKMIYKGSMLEDLPNAVMVFGYTNASWTL
                     KADMISEYVCRLLKHMDKTGTRYCLPNNSDTSVEKTPFLDMRSGYIQRALGRVPYQGS
                     KAPWRLYQNYAMDYALLRFGKLNDGVIKFA"
BASE COUNT          506 a          363 c          314 g          487 t
ORIGIN      
        1 ggagctattc cgctgtcatt tagtaattta tttgtcaaac aacaacccta tcaaatgttg
       61 gtaaatttac gtcgttattt gcaagaaaat ccgaataaac aattagcgag taattaaaaa
      121 aaggcgcata ttgcgccttt tttattaagc aaatttaatc acaccatcat tcaacttgcc
      181 aaagcgtaat aaggcataat ccatcgcata gttttgatat aaacgccacg gtgcttttga
      241 accttgatag ggaacacggc ctaaagcacg ctggatgtaa cctgaacgca tatctaagaa
      301 cggtgttttt tcaacgctgg tgtcactatt attaggcaaa caataacgtg tgcctgtttt
      361 gtccatgtgt tttaataaac ggcaaacata ttcactaatc atatcggctt ttaacgtcca
      421 agaagcattg gtataaccaa acaccatcac cgcattaggt aagtcttcta acatcgagcc
      481 tttataaatc atctttttag tcacatcaaa agcttgaccg tctttttcta actggatgcc
      541 acctaataat ttaatgtcta aacctgtcgc agtaataata atatctgctt caagggtttt
      601 gcctgatttt aataaaatgc catcctcagt aaaggaatca atatgatcgg tgacaaccga
      661 ggctttgccc ttacgcaaca ctttaaataa atcaccattg ggcacagcac acaaacgctc
      721 atcccacgga ttgtattggg gcgtaaaatg gctcatatca aaatcttggc caagctgacg
      781 gcgcacttgt gacaataaga aacgacgcac caccgcaggt ttagcacgac tataacgata
      841 caagcctaaa gataagccga cattacgcac tcgtgccatg tgatacacca ctttttcggg
      901 taaaaatttg cgtaacttgt tagaaataat gtcttcttgg ggaacagaaa tgacatagct
      961 cggagaacgt tgcaacatag taacgtgtgc ggctttatcg gtcattgacg gcaacaaggt
     1021 tacagccgtt gccccactac caataatcac cactttttta ttggtgtaat ctaggttttc
     1081 tggccaaagt tgagggtgaa taatttgacc tttaaaccgc tcacggcctt taaattcagg
     1141 ggtatagcct gcttcgtagt tataataacc tgtacaggaa atgacaaaat tggcttcgat
     1201 ggtgacaggc tcgcctgctt tgttggttgc ttcaattgtc catgctgctt tgggggttga
     1261 ccacgccgct ttgagcactt tatggccata acgaatatga cgattgacat catattcttg
     1321 cgcagcttca tgaatataag cacgaatcga tgggccatcg gcaatcgctt ttggattggt
     1381 ccacggttta aagttatatc ccaaggtgta catatcagaa tctgagcgta taccagggta
     1441 acggaataaa tcccatgtgc cacccatcgc atcacggcct tctaaaatga caaaagtttt
     1501 attaggacat tcacgtttta aatgacaagc tgcaccaatg cccgataatc cagcaccaat
     1561 aattaagaca tcaaacttct cggccatgat tttaccctca aaattttttt gattgattta
     1621 agttcatgaa ccaattctga catcaagcgt tgtcaacttc tagtgtcttt
//
LOCUS       DAOQMB010000510         1668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54736_length_1668_cov_17.694978, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000510 DAOQMB010000000
VERSION     DAOQMB010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54736_length_1668_cov_17.694978"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /locus_tag="PLY05_11585"
     CDS             <1..1545
                     /locus_tag="PLY05_11585"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HNC03980.1"
                     /translation="QVEGGIFKYTYSEAAKRAKQVANALTQKLGIQQGDRIGTLAWNT
                     YRHYELYFGISGIGAITHTINPRLFPEQIIYIINHAADRFIFVDLTFLPLLEAVASHI
                     SKVEGVVVLTDKAHMPASSKLNNLLCYEELIADASTEYTWPALDENADAALCYTSGTT
                     GNPKGVMYTHRSTVLHAMAAAQYNALNITPDTILLPVVPMFHVNAWGTPYAATMMGAK
                     LVLPAHGMDGASLYELINGEQANLLLGVPTVWLGLLNYLDSINAKLDSVKNVVVGGSA
                     APLSMIKAFQEKHDAFLIHAWGMTELSPLGTVNSMTRNMAALPLEQRYQAQLKQGRPV
                     YGVEIKIVDDNGTELARDGKVFGRLLVRGPWVVDSYYLNEDRKSFVDGWFDTGDVATI
                     DSDNTMQIVDRSKDVIKSGGEWISSIDLENAAVGHPALKECCVIGVYHPKWDERPLLL
                     AIKKDGAEVSEAEVISFLSDKVAKWQLPDAVVFVPDLPHTATGKLLKTGVRETYKNYL
                     VESGKA"
BASE COUNT          471 a          330 c          384 g          483 t
ORIGIN      
        1 caagttgaag gcggtatttt taaatatacc tatagcgaag cggcaaaacg cgcaaagcaa
       61 gttgccaatg ccctcaccca aaaattaggc attcaacaag gcgaccgtat tggcacgttg
      121 gcatggaaca cttatcgtca ttacgaatta tattttggca ttagtggcat tggtgcaatt
      181 actcatacca ttaacccacg tttatttccc gaacaaatta tttatattat taatcatgcg
      241 gctgatcgtt ttatttttgt cgatttaacc tttttaccct tactcgaagc ggtcgccagc
      301 catattagca aggtggaagg tgtggtggtg ttaaccgaca aagcgcacat gccagccagt
      361 agcaaattaa acaatttatt atgttatgaa gaattaattg ccgatgccag taccgaatac
      421 acatggccag ctttagacga aaatgccgat gcggctttat gttatacctc tggcacaaca
      481 ggaaatccta aaggggtgat gtacacgcat cgttccaccg tattacacgc gatggccgct
      541 gctcaatata atgcgttaaa cattacgcct gacacgattt tattacctgt tgtaccaatg
      601 tttcacgtta acgcatgggg aacaccctat gccgcgacga tgatgggcgc aaaattggta
      661 ttacctgcac atgggatgga tggtgcgtcg ttatatgaat taattaatgg tgaacaagcc
      721 aatttattgt tgggtgtgcc gactgtttgg ttaggtttat taaattattt agattcgatt
      781 aatgccaaat tagactcggt taaaaatgtg gtggttggcg gttcggctgc accgttgtcg
      841 atgattaaag ccttccaaga aaaacacgat gcctttttga ttcatgcatg gggtatgacc
      901 gaattaagcc ccttgggtac agtcaacagc atgacccgca acatggctgc cctcccctta
      961 gaacaacgtt atcaagctca attaaaacaa ggtcgccctg tgtatggggt ggagattaaa
     1021 attgtcgatg ataacggcac agaactcgcg cgtgatggca aagtatttgg ccgtttactg
     1081 gtcagagggc cgtgggtggt ggatagctac tacttaaatg aggatagaaa atcctttgtt
     1141 gatggttggt ttgatacagg tgatgtggcc acgattgaca gtgataacac tatgcaaatc
     1201 gttgatcgtt ctaaagacgt gattaaatca ggcggtgaat ggattagctc gattgattta
     1261 gaaaatgcgg cggtgggtca tcctgccctt aaagagtgct gtgtaattgg tgtttatcat
     1321 ccaaagtggg atgaacgtcc gttgttatta gccatcaaaa aagacggtgc agaagtctct
     1381 gaagccgagg tcattagctt tttaagtgat aaagtcgcta aatggcaatt acctgatgct
     1441 gtcgtgtttg tacccgattt accccacacc gcaacaggta aattattaaa gacaggcgtt
     1501 agagaaacgt ataaaaacta tttagtagaa agtggcaagg cttgatactc aactttattt
     1561 tttgatgccg tcccctcttc tcttaggaga gggctagggt aagctcgtca cgttctaatc
     1621 atccctcttt cccacaacag cttaaatcct tgttgaatat tagttaat
//
LOCUS       DAOQMB010000511         1666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54878_length_1666_cov_5.887027, whole genome shotgun sequence.
ACCESSION   DAOQMB010000511 DAOQMB010000000
VERSION     DAOQMB010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54878_length_1666_cov_5.887027"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLY05_11590"
     CDS             complement(<1..386)
                     /locus_tag="PLY05_11590"
                     /inference="COORDINATES: protein motif:HMM:NF014404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC03981.1"
                     /translation="MDISKGQRTPISQLVTGQSFQLAVSTVGAVIVDISCFGVDAAQK
                     LSDERYMTFFNQPKTPCGGVAIVPSIGKDTTTFHLNLALLPASIDRLVITAAIDGTGT
                     MNQLRDGCYLRFLANGQESSRFNFSG"
     gene            complement(399..>1666)
                     /locus_tag="PLY05_11595"
     CDS             complement(399..>1666)
                     /locus_tag="PLY05_11595"
                     /inference="COORDINATES: protein motif:HMM:NF021641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC03982.1"
                     /translation="SQLVTGQSFQLAVSTVGAVIVDISCFGVDAAQKLSDERYMTFFN
                     QPKTPCGGVAIVPSTGKDTTTFHLNLALLPTTIDRLVITAAIDGTGTMNQLRDGCYLR
                     FLTNGQESSRFNFSGQDFAQEKALMLGELYRKNGDWRFCATAQGFNGGLEALVKHFGG
                     SVAAPATSVTAAISSPVVEQPKSPPKLSLEKKVAKDAPQLLSLAKKAQISLEKKKLGD
                     VQAQVALVLDASGSMFHQYFDGTVQKVIERILPLAVHFDDDGALDCWGFGCLAYKLDS
                     VTLKNVKNYTKHQRLDDDRGLSRFDIGNDEPVVIKEIIKYYKKSKSSLPAYVVFISDG
                     GVGSDDEIEELLRESASLPIFWQFVGIGGSDYGILENLDTMMGRVVDNCNFFALDDLH
                     QISEQELYDRLLEEFPQWLTAAKAKSIIR"
BASE COUNT          512 a          378 c          292 g          484 t
ORIGIN      
        1 tggccactaa aattaaaacg tgaactttct tgaccattgg ctaaaaagcg taaataacaa
       61 ccgtcacgta attgattcat cgtgcctgtg ccatctatcg cggcggtaat cactaaacgg
      121 tcaatcgagg caggtaataa cgctaaattt aagtgaaagg tggtggtatc tttgcctatg
      181 gatggcacaa tcgctacccc accacaaggc gttttgggtt gattaaaaaa cgtcatatat
      241 cgctcatcac tgagcttttg tgcggcatct acaccaaaac agctaatatc aacaatgacc
      301 gcaccgactg tactcacagc cagttgaaac gattgacctg tcactaattg gctaatcggt
      361 gtacgttgtc ctttgctaat gtccatataa tttgctcctt atctaataat acttttagct
      421 ttggcggctg taagccattg aggaaattct tctaacaatc tatcgtacaa ctcttgctcg
      481 ctaatttgat gtaaatcatc taaagcaaaa aaattacaat tatctaccac acgtcccatc
      541 atcgtatcaa gattctctaa aataccatag tcagaaccgc caatgcctac aaattgccaa
      601 aaaataggta aggaagcaga ctctcgcaat agctcttcaa tttcatcatc gctaccaaca
      661 ccaccatcac taataaatac aacataagct ggtaaggatg atttagattt tttatagtat
      721 ttaataatct cttttataac tactggctca tcatttccaa tatcaaagcg actcagtcct
      781 ctatcatcat ctaaacgttg atgttttgtg tagtttttga cattttttaa agtaacagag
      841 tccaatttat atgccaagca gccgaatccc caacaatcta atgcaccatc atcatcaaaa
      901 tgaaccgcta atggtaatat cctttctatc actttctgaa ctgtgccatc aaaatactga
      961 tgaaacatag agccagaggc atctaacact aatgctactt gtgcttgtac atctccaagc
     1021 tttttctttt ctaaactaat ttgtgctttt ttagcaagtg acaataattg tggtgcgtct
     1081 ttagcaacct ttttttctaa tgacagtttc ggtggagatt ttggttgttc cacaacagga
     1141 gaagatattg ctgctgtaac ggatgttgca ggggcagcca cagagccgcc aaaatgtttc
     1201 actaaggctt ctaaaccacc attaaaccct tgtgcggtgg cacaaaaacg ccagtcgcca
     1261 ttttttcgat acaactcacc caacattagg gctttttctt gggcaaaatc ttggccacta
     1321 aaattaaaac gtgaactttc ttgaccattg gtcaaaaagc gtaaataaca accgtcacgt
     1381 aattgattca tagtgcctgt gccatcaatc gcggcggtaa tcactaagcg gtcaatcgtg
     1441 gtaggcaaca aggctaaatt taagtgaaag gtggtggtgt ctttgcctgt tgatggcaca
     1501 attgccaccc caccacaagg cgtttttggc tgattaaaaa aagtcatata gcgctcatcg
     1561 ctgagctttt gggcggcatc tacaccaaaa caactaatat caacaatcac agcaccgact
     1621 gtactcacag ccagttgaaa cgattgacct gtcactaatt ggctaa
//
LOCUS       DAOQMB010000512         1665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_54936_length_1665_cov_17.993789, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000512 DAOQMB010000000
VERSION     DAOQMB010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54936_length_1665_cov_17.993789"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..915)
                     /locus_tag="PLY05_11600"
     CDS             complement(124..915)
                     /locus_tag="PLY05_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC03983.1"
                     /translation="MTKPILHFAHANGVPSACYQKMLAILAEDFQVVMIPVIGIDPNY
                     PVDNHWWSLTEQVAESIKRQSDGQPVYVLGHSLGAMTSYMVAHRFPHLVKALVMLDPP
                     MFNGMDAYKLHLAKLLGKAENLTPAAKSKNRRDVWPNRAEAAASLRPKSLFKTFDEQC
                     FADYINYGLKDCANGVCLTIPVSTEVAIFNNVPTNTWRFRKPLAMPAAIVVGKDSHFA
                     KTGYPERLAQEQPVSLHYTQGGHMFPLEYPLETAAFVKELFKRLK"
     gene            1016..1351
                     /locus_tag="PLY05_11605"
     CDS             1016..1351
                     /locus_tag="PLY05_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03984.1"
                     /translation="MKLFIMSLMSMLLLSACSQEQQNKISRSLQNWTGTNGVLEIYAG
                     DKLVRRFINIDKMTTATGTDDSNLRPYRFGYGILDENLNMVADAGEKKVYFEVSDYST
                     PYVFFEAPK"
     gene            complement(1384..>1665)
                     /locus_tag="PLY05_11610"
     CDS             complement(1384..>1665)
                     /locus_tag="PLY05_11610"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HNC03985.1"
                     /translation="EFPDESDEGLSVQQQDDGSWLVDALIDAHRISQLLNYNLVDDNN
                     RYSTLAGFLLAELGHLPEQGEQIIKEDLCFEVVAVTNTQIERVRISRQS"
BASE COUNT          536 a          344 c          291 g          494 t
ORIGIN      
        1 gctaataatt ttttagtcat gattcgctct caagctacaa tttgttatct tgaggcgatc
       61 ataactaaaa ccattaacaa taaaaaagaa tatataaatc tatttttata acaaaaaaga
      121 atatcacttt aaacgtttaa agagttcttt cacaaaagca gccgtttcta agggatactc
      181 taaaggaaac atatgtcccc cttgcgtata atgtaatgag actggctgct cttgtgccaa
      241 gcgttcagga tagccagttt tggcaaaatg gctatctttg ccaaccacaa tcgctgctgg
      301 catcgctaaa ggctttctaa atcgccatgt gttagttggt acattattaa aaatagccac
      361 ttcggtgctt acaggaatcg tcaaacacac tccattagca caatccttta gaccataatt
      421 gatataatct gcaaaacatt gttcatcaaa ggttttaaat aaacttttag gtcgtaaact
      481 agcagccgct tcggcacgat taggccaaac atctcgacga tttttacttt tcgctgcggg
      541 tgtcaaattc tctgctttgc ctaataattt ggcaaggtgt agcttatagg catccatacc
      601 attaaacatc ggcggatcta acatgaccaa agctttgact aaatgtggaa aacgatgcgc
      661 caccatataa ctggtcattg cccctaaaga atgacctaaa acatagactg gctgaccatc
      721 actttgtcgt ttaatggact ctgcaacttg ctctgttaat gaccaccaat gattatccac
      781 gggataattg gggtcgatac caatcacagg aatcataacg acttgaaaat cctctgctaa
      841 aatagctaac attttttgat aacacgccga tggcacacca ttcgcgtggg caaaatgcaa
      901 aataggcttg gtcattgtca aaaactctta cagttacaat ctcaaattga ctgtaccaca
      961 agaatcttta agatggccgt gacttagcag ttctattaca aggaaactaa aaccgatgaa
     1021 gcttttcatt atgtcgctga tgagtatgtt attactcagt gcctgctccc aagagcaaca
     1081 aaataaaatc agccgtagct tgcaaaattg gactggcaca aatggtgtat tagagattta
     1141 tgctggtgac aaactggtta gacgctttat taatattgac aaaatgacca ctgcaacagg
     1201 tacagatgat agtaatcttc gtccttatcg gtttggttat ggtattttag atgaaaactt
     1261 aaatatggtt gctgatgcag gcgaaaagaa agtctatttt gaagtcagtg actacagcac
     1321 gccgtatgta ttttttgaag ccccaaaata aattaaagga ctgaatattc agtcctttaa
     1381 attttagctt tgacgactaa ttctcactcg ctctatttgg gtgttagtca ccgccaccac
     1441 ttcaaaacat aaatcctctt taataatttg ctcaccttgt tcgggtaaat gaccaagctc
     1501 cgctaataag aatcctgcca atgttgaata acgattatta tcatccacta aattatagtt
     1561 taataactgt gaaatacgat gagcatcaat taacgcatcc actaaccaag aaccatcgtc
     1621 ttgttgttgc acagaaagcc cttcatcgct ttcatcagga aactc
//
LOCUS       DAOQMB010000513         1663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_55098_length_1663_cov_3.358209, whole genome shotgun sequence.
ACCESSION   DAOQMB010000513 DAOQMB010000000
VERSION     DAOQMB010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55098_length_1663_cov_3.358209"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLY05_11615"
     CDS             <1..505
                     /locus_tag="PLY05_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03986.1"
                     /translation="DGGKTWQSLMAQVQNPNFLHLYAITGHKGQAFIAGEQGTLLKWN
                     DSSQTFDALNSPYKGTFFGLLSASEVLLAYGLRGNLVRSTDNGTTWRQIDTYTPAGLV
                     DGAILDNGQLILVSQRGDILLSKNQGISFYRPTVNQRLPFTSVLPLNKQQVVITSLKG
                     VKVEDLK"
     gene            528..>1663
                     /locus_tag="PLY05_11620"
     CDS             528..>1663
                     /locus_tag="PLY05_11620"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03987.1"
                     /translation="MSKPNIRNMPVVKDLSEFDKQSGNILERLIFNYRQVVVAICLLA
                     TLVFVYLIQSRLELNASFEKIMPQSHPYIQNYLQNKDQLRGLGDSLRIVVENPKGTIF
                     DAEYLETLRQINDEVFLVSGVDRAWMKSLWMPVVRWTEVTEEGFTGGPVIPESYDGSL
                     ASLSLVTQNVSRAGIAGSLVANDFRSSMIFVPMLAVDADGNRVDYKKLRTQLETIRAK
                     YEKEGKVKIYIVGFSKLIGDLMAGLEKIMLYFLIAAIIAAIIILLYTKCLRSTVLVLF
                     CSFIAVLWQLGLIAALGFEIDPYSILVPFLVFAIGVSHGAQKMNGIMQDVGRGTHQLV
                     AARYTFRRLFLVGLTALLADAVGFAVLMVIDIPVIRELALTASIG"
BASE COUNT          472 a          258 c          365 g          568 t
ORIGIN      
        1 agatggtggc aaaacatggc aatcactgat ggcacaagta caaaatccca attttttgca
       61 tttgtatgcc attacgggtc acaaaggaca agcttttatt gcaggtgaac aaggcacttt
      121 attaaagtgg aatgatagca gccaaacatt tgatgcctta aatagtcctt ataaaggcac
      181 attttttggt ttgttaagtg cttctgaggt gttattggcc tatggtttac gtggcaacct
      241 tgtccgtagt accgataatg gcactacatg gcgacaaatc gacacctata cccctgctgg
      301 tttggttgat ggcgcaattt tagataatgg tcaactgatt ttagttagtc aacgtggtga
      361 tattttatta tctaaaaatc aaggcataag tttttatcgg cctacagtta atcagcgatt
      421 accttttacc agtgttcttc ctctgaataa acaacaagtg gtgataacca gtttaaaagg
      481 tgtgaaagta gaagacctaa aataatactc accaagagct ataaattatg agtaagccaa
      541 atatccgtaa tatgcctgtc gttaaagatt tatcggaatt tgataaacag tcaggtaata
      601 tattagaaag attaattttt aattatcgtc aagtggtggt ggctatttgt ttattagcca
      661 cccttgtttt tgtttatttg attcaaagtc gtttagagtt aaacgctagc tttgaaaaaa
      721 taatgccgca aagtcatcct tatattcaaa actatttaca aaataaagat cagttgcgtg
      781 gtttgggtga ttctttacgt attgtggttg aaaaccccaa aggtactatt tttgatgctg
      841 aatatttaga gactttacgc caaattaacg atgaagtatt tttggtatcg ggtgtcgata
      901 gagcatggat gaagtctttg tggatgcctg tggtcagatg gacagaggtt accgaggaag
      961 gatttacagg cggcccagtt attcctgaaa gttatgatgg ttctttggct tcgttatcct
     1021 tagtcacgca aaatgtgtca cgtgcaggca ttgctggtag tttggtcgcc aatgattttc
     1081 gttcaagtat gatttttgtg ccgatgttag ccgtagatgc agatggtaat cgtgttgatt
     1141 ataagaagtt gcgtacacaa ttagaaacca ttcgtgctaa atatgaaaaa gaaggcaaag
     1201 ttaaaattta tattgtgggt ttttctaaat taattggcga tttaatggct ggtttagaaa
     1261 aaatcatgct ctatttttta attgcggcaa tcattgcggc aattattatt ctgctttaca
     1321 ccaaatgttt acgcagtact gttttggtct tattttgttc atttattgcc gtattatggc
     1381 aactaggttt aattgctgcc ttagggtttg agatagatcc ttattcgatt ttagtgccgt
     1441 ttttagtatt tgcgattggc gtgtcgcatg gcgcgcaaaa aatgaacggt attatgcaag
     1501 atgtaggacg tggtacgcat caattagtag cggcacgtta tacctttaga cgattgtttt
     1561 tggtgggatt aaccgcgtta ttagctgatg cagttggttt tgcagtgtta atggtcattg
     1621 atattcctgt tatcagagaa ttagcactca ccgccagtat tgg
//
LOCUS       DAOQMB010000514         1662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_55133_length_1662_cov_18.598631, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000514 DAOQMB010000000
VERSION     DAOQMB010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55133_length_1662_cov_18.598631"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /gene="ruvX"
                     /locus_tag="PLY05_11625"
     CDS             complement(<1..390)
                     /gene="ruvX"
                     /locus_tag="PLY05_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004920504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HNC03988.1"
                     /translation="MLESVNSVLGFDFGTKKAGVAIGQQITASASPLSPLVMRDGIPE
                     WSVIEQLIKEWQPQLLLVGLPLNMDDSESELSHLARKFARRLHHRFNLPVLMVDERLT
                     SRSAKEMLKDIGQQRKGKLPSLDSTAAV"
     gene            complement(383..952)
                     /locus_tag="PLY05_11630"
     CDS             complement(383..952)
                     /locus_tag="PLY05_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HNC03989.1"
                     /translation="MTLANSNFTHHCLIAMPDMEDSRFSETVTYIVKHDAEGAVGLVI
                     NKPLELSLTQLLQEINLPILKGIDEPEMPVLYGGPVSSEAGFVLHHERGEWNSSLEVE
                     DGLCVTSSRDILDAIAQGKGPKNYLVLLGYAGWSAGQLEKEMAENAWLVCPADNSLLF
                     ELAFEDRWQAAASRIGVNLSLLSHQVGHA"
     gene            1106..>1662
                     /locus_tag="PLY05_11635"
     CDS             1106..>1662
                     /locus_tag="PLY05_11635"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC03990.1"
                     /translation="MTTLATVITGASSGIGEALAHLFAREQQALVLVARRKDKLEGLA
                     KALNSKYGVKVHVIALDLQQIGAAAQLMNEVSNLKLSVDTLVNNAGFGVSAPFAKSDA
                     SQTSGMMHLNMVALTELTHMVLPMMLERGHGRIMNIASVVAFQPCPYFAVYAATKAYV
                     LSFTEALAEEMRGRGILVTAVCPGST"
BASE COUNT          495 a          346 c          325 g          496 t
ORIGIN      
        1 cacggcggca gttgagtcta aagaaggtaa tttgcctttg cgttgttgac caatatcttt
       61 gagcatctct ttggcagaac gactggttaa acgctcatca accattaata caggcaaatt
      121 aaaacgatga tgcaagcgtc gagcaaattt tcttgctaaa tgggataatt ccgactcact
      181 atcatccata tttaatggca aacctaccaa cagcaattgt ggctgccact ctttgattag
      241 ttgttcaata acagaccatt caggaatacc atctcgcatc accaatggag acaaagggga
      301 tgcacttgcc gtaatctgtt gaccaatcgc cactcctgct ttttttgtac caaaatcaaa
      361 gcccaaaaca ctgtttactg attcaagcat ggccaacctg atgacttaat aagcttagat
      421 ttacaccaat tcgtgaagca gcggcttgcc aacgatcttc aaaagctaat tcaaataata
      481 aactattatc cgcaggacaa actaaccatg cgttttcggc catttctttt tcgagctgtc
      541 ctgctgacca tcctgcataa cccaataata ctaaataatt ttttggccct tttccttgag
      601 caatggcatc aagaatatcg cgtgaactgg tgacacataa accatcctca acttctaaac
      661 tggaattcca ctctccacgt tcgtgatgca gcacaaaacc tgcctcgcta ctaacaggcc
      721 caccgtataa aacgggcatc tctggctcat caattccttt taaaattggt aaatttattt
      781 cttgtaacag ttgggttaaa gataattcta gtggtttgtt aatgactaaa cccacagcac
      841 cttcagcatc atgtttaaca atataagtca ctgtttcaga aaaacggcta tcttccatgt
      901 cgggcatggc aatcaagcaa tgatgagtaa aattagaatt agctagggtc atattcaata
      961 attagttggc tacataaaga atttttaatt taacacaatt gatttaagtc acaagcttga
     1021 tttagttttg tttagcgagt atttaggtta atgccgattg atatagcttt tttggacaat
     1081 aaagcaacaa ctattgagaa taaagatgac aacattagca accgtgatta ctggcgcatc
     1141 cagtggtatt ggcgaagcac tagcccattt atttgcacgt gaacaacaag cattggtctt
     1201 ggttgcacgt cgtaaagata aattagaggg tttggctaaa gcattaaata gcaaatatgg
     1261 cgttaaagtt catgtgattg cgcttgattt gcaacaaatc ggggctgctg ctcaactaat
     1321 gaacgaagta tcgaatctaa aactatcagt tgatactttg gtcaataatg caggttttgg
     1381 cgtgagtgct ccgtttgcta aaagtgatgc gagccaaaca tcgggcatga tgcacttaaa
     1441 tatggtggcc ttgaccgaat taacacatat ggtgttgcca atgatgttag agcgtggtca
     1501 tggtcgcatt atgaatattg cctctgtggt tgcttttcaa ccgtgccctt attttgcagt
     1561 ttatgccgcc acaaaggcct atgtgttatc gtttaccgaa gccttagccg aagaaatgcg
     1621 tggtcgtgga attttagtca ctgccgtttg tcctggttca ac
//
LOCUS       DAOQMB010000515         1652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_55786_length_1652_cov_15.221039, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000515 DAOQMB010000000
VERSION     DAOQMB010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55786_length_1652_cov_15.221039"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /gene="dnaX"
                     /locus_tag="PLY05_11640"
     CDS             complement(<1..980)
                     /gene="dnaX"
                     /locus_tag="PLY05_11640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNC03991.1"
                     /translation="MSYQVLARKYRPKNFAELVGQEHVARALANALTQNRLHHAYLFT
                     GTRGVGKTTIARILAKCLNCETGITAQPCGVCATCQEINQGRYIDLIEIDAASNTGVD
                     DVREVIENAQYMPTRGRYKVYLIDEVHMLSKAAFNALLKTLEEPPSHVKFLLATTDPQ
                     KIPVTVLSRCLQFSLKPMSPERVVQHLENVLAAENVQYDMAALWELGRAANGSMRDAL
                     SLTDQAIAFGNGLLREQEVRDMLGSVDRDLVFRVLAAIDSADAGQVLAVINTLAEQSV
                     DFQGALAALISMLHRVALAQLLPDAIENTEGDKEKVLALAQALTPEDVQL"
     gene            1168..>1652
                     /locus_tag="PLY05_11645"
     CDS             1168..>1652
                     /locus_tag="PLY05_11645"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC03992.1"
                     /translation="MKTKTILITGGESGVGAATARALAAQGHKVLITSRDAEDGAQLC
                     QEINTAFPRANIKNLVLDLSSFSSIRLFCSKLEKEYDAIDVLINNASLAPLTKQKTRD
                     GFEAQFGANYLGHFLLTQLLLDLLNEAGKGRIVHTSSMMHWLGNIDFGSFHGEKFYDP
                     IR"
BASE COUNT          476 a          373 c          317 g          486 t
ORIGIN      
        1 tataactgta catcttcagg ggttaatgct tgggctaaag ccaacacctt ttctttgtca
       61 ccttcagtat tttcaatagc atcaggtaac aactgtgcta atgcaacacg atgtagcata
      121 ctaattaatg ctgctaatgc cccttgaaaa tcaacacttt gctctgccaa ggtattaatc
      181 acggctaaca cttggccagc atccgcacta tcaatggcag ctaatacccg aaacaccaaa
      241 tctcggtcaa cactgcccaa catatcacgc acttcttgct cgcgtaataa accattacca
      301 aaagcaatcg cttgatcggt caacgacagc gcatcacgca ttgaaccatt tgcggctcgt
      361 cctaactccc ataacgcggc catatcgtat tgcacatttt ctgcggctaa tacattttct
      421 aaatgctgca ctacccgttc tggcgacatc ggctttaacg aaaactgcaa acagcgtgat
      481 aacactgtga caggaatttt ttggggatca gtggtggcta ataaaaactt aacgtggctg
      541 ggtggttctt ctaaggtttt gagcaaggca ttaaaagctg ctttcgacaa catatgtact
      601 tcgtcaatta agtacacttt ataacgtcca cgggtgggca tatattgcgc attttcaatc
      661 acttcacgca catcatcaac gcctgtatta ctcgccgcat caatttcgat taaatcaata
      721 taacgacctt gattgatttc ttgacaagtg gcgcatacac cacaaggttg cgcggttatg
      781 cctgtttcac aattcagaca tttagccaaa atacgcgcaa tggtggtttt gccaacgccg
      841 cgagtacctg taaatagata agcgtgatgc aaacgattct gtgttaaagc attcgctaat
      901 gcacgagcaa catgttcttg cccaactaac tcagcaaagt ttttcggacg atatttgcgc
      961 gcaagaactt gataactcat ctttaggtaa aactcttatt aattatttaa ctgaaatacg
     1021 ggcttagact ccctcaatca taacataaag cccttccaga aattgataag ctttatgctt
     1081 gcttgtcgtt ttaatgcttg tgcttcatct tggcacacat taggataagg ctatgtccca
     1141 taatcgctca attgttatat taacacgatg aaaaccaaaa ctattttaat tacaggcggt
     1201 gaaagtggtg ttggtgcggc aacggcgcgc gcccttgctg ctcaaggcca taaagtgctt
     1261 attaccagcc gtgatgctga agatggggcg caattatgcc aagaaattaa taccgctttt
     1321 cctcgtgcca atattaaaaa tttggtttta gacctgtcgt ctttttcttc gattcgtttg
     1381 ttttgtagca agctagaaaa agaatacgat gcaattgacg tattaattaa taatgccagc
     1441 ctagcaccac tcaccaaaca aaaaactcgc gacggttttg aagcacaatt tggggcaaat
     1501 tatttaggcc attttttatt aacccagttg ttactcgatt tgttaaatga agcaggcaaa
     1561 ggccgcattg tccatacttc gtcgatgatg cactggttgg gtaatattga ttttggtagc
     1621 tttcatggcg aaaagtttta tgacccaatt cg
//
LOCUS       DAOQMB010000516         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_55997_length_1649_cov_13.861355, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000516 DAOQMB010000000
VERSION     DAOQMB010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55997_length_1649_cov_13.861355"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..1479)
                     /locus_tag="PLY05_11650"
     CDS             complement(187..1479)
                     /locus_tag="PLY05_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HNC03993.1"
                     /translation="MINVKKVRLKVMKSSKNTANNLSVLKTSQAVVHISHSISLRQYK
                     YWILLLHFYREALQEGTESRQDVLYRVPLSKLTEYLGYEPVKNELRDDFEVLRKEPII
                     INILSKDGHKAQRGMGFISEWEISSKTVGFRLPSFLQEVMKGLDDAKAIFHLLHWEIF
                     SSFNGKYEAILYKLCKDYAGVKRTPYMTIAEFRHYIGLQENEYTQFAELNRWTISKPL
                     KVINDSIVSDIAIEVEFNRNGRKAEGLHFKVKQKQQKLSIASITETTKPTQLAFAQAK
                     ISIPNDEQEDYLQKHGAELVMATIERANLYIDDKLAKGENVNIGAIYSSAFAGNWGVQ
                     YLQQKELQVKQAAKTIKSKTTKTQAKTVLSSNDKEQILQRWQDLPINEKQDYFKRALN
                     NIPKVMKSIEQEKATIDNAMSRPLLRIELEKLLSTVAN"
BASE COUNT          491 a          318 c          278 g          562 t
ORIGIN      
        1 ttgatggcat catgaaaatc taaattcccc tgacacagaa taatgaatac cctccgacaa
       61 gacagtaaag ttcggtaaga ttatccatga gcgtaccttt tgtgtggggc tagttttgtg
      121 tgtcgttacc caaactctaa cccttttcaa aggtacgttc cttatctttc ttatcccgaa
      181 ctcaggttaa ttagcaacag ttgataataa tttttcaagt tcaatacgca acaaagggcg
      241 actcatggca ttgtcaatcg ttgctttttc ttgctcaata gatttcatca ctttgggtat
      301 gttatttaag gcacgcttaa aataatcttg cttttcatta atcggtaaat cttgccatcg
      361 ctgtaaaatt tgctctttat cattgctaga aagtactgtt tttgcttgag ttttagtggt
      421 ttttgatttt atggttttgg cagcttgttt tacttgtaat tctttttgtt gcaaatattg
      481 cacaccccaa ttacctgcaa aagcactgct ataaattgca ccaatattta cattttcacc
      541 tttggctaat ttatcatcaa tatataaatt agcacgctca atggtcgcca tcaccaattc
      601 agccccatgt ttttgcaaat aatcttcttg ctcatcatta ggaatactaa tcttagcttg
      661 ggcaaacgct agttgtgttg gtttagtcgt ttcggtaatt gaggcaatcg ataacttttg
      721 ttgtttctgt tttactttaa aatgcaaacc ttctgcttta cgaccattac gattaaactc
      781 aacctcaatg gcaatatccg acacaatact gtcgttaatc acttttaatg gtttagaaat
      841 tgtccaacga tttaattcag caaactgagt atattcgttc tcttgcaaac caatataatg
      901 acgaaattca gcaatagtca tgtaaggcgt ccgctttacc ccagcataat ccttgcaaag
      961 tttgtataaa attgcctcat atttaccgtt aaaactgcta aaaatttccc aatgtaataa
     1021 atgaaaaatt gcttttgcat cgtccaatcc cttcataacc tcttgcaaaa aagaggggag
     1081 acgaaaacct accgttttac tagaaatttc ccattccgaa ataaaaccca taccacgttg
     1141 tgctttatgg ccatctttag acaagatatt gataataatt ggctctttac gtaacacctc
     1201 aaaatcatct cttaactcat ttttaacagg ctcatagcct aaatattcag ttaatttgct
     1261 aagaggtaca cgatacaaaa catcctgacg agattcggtg ccttcttgaa gtgcttcacg
     1321 ataaaagtgc aatagcagta tccaatattt gtactgacgc aaactaatac tgtggctgat
     1381 gtgtaccacc gcctgtgatg tttttagaac agaaagatta tttgctgtat tttttgacga
     1441 tttcattact ttaagacgca cttttttaac gttaatcata cgcctatatc atgtagaaag
     1501 gcaagaagaa ttactacatg atagattgtt aaaaaaccaa ctcttgagca attttaatca
     1561 ttaagggggg ggtgattttc tacatgataa ctaggggggt ggatttctac atgatagggg
     1621 ggtgattctc tacatgatag gggggtgga
//
LOCUS       DAOQMB010000517         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_55998_length_1649_cov_9.296738, whole genome shotgun sequence.
ACCESSION   DAOQMB010000517 DAOQMB010000000
VERSION     DAOQMB010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55998_length_1649_cov_9.296738"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1649
                     /locus_tag="PLY05_11655"
     CDS             259..>1649
                     /locus_tag="PLY05_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNC03994.1"
                     /translation="MSEVLEHGLNIRQARIQETRSYLARTHELFEQFGSQELKTSHAK
                     FEELKKALDSDSQVLLVVIGEFSRGKSSLVNALLGIHLLRSAQEATTAINTFVKAIPE
                     GRNERFIRIHYQDGRTPDEITWDSDDVLEKWGTELNSDHADARKLVDYIEVFTSHPLL
                     DKGLVLIDTPGLQSVVQHHEEITRRAIAEAHIAIWVQSTAQLGGNATEWQFLSQTIRK
                     NFRKFITVINMWDAVLEPTDYHEQKKSEDVRVYEKLERVRQNFRKNLFGIAEDELEVL
                     TNPEHLMGVSALWQLSHDPQKQARSGVDKLAERISVMFSSGEALEQIYLKPLQQLSHI
                     QEQLIGSIVDELEQLASNDTLEERQRAADNLSKDIKILEQDMQQENNSAKQEHNNAAK
                     VLASQIKDQLIQPLKYLKQDIETQVDRSYIEKLLQQKKAKISLPPELDSEFEAVGKQV
                     AHSWELQKNQLGKT"
BASE COUNT          570 a          271 c          369 g          439 t
ORIGIN      
        1 tgtttggatt gtggctggag ctgcacacgc aaccaaaaaa gtgatgtgct aatgctgcca
       61 aaagtgtgtg ggaagtgtgg ggggaagagg ttggatgtaa gcatggtcaa tatgccaaag
      121 tccttactcc aaaagctctt aaataattgg cgttagttat cagtataaaa aggtaattca
      181 tcagctgttc caaacgattt tagatgatat tgatatatta agataaaact aaaatttgat
      241 tttatgagga gttttataat gagtgaagtt ttagagcatg ggttaaatat ccgtcaagca
      301 cgtattcaag aaacacgtag ttatctagca agaacccatg agctatttga acagtttggc
      361 agccaagagc taaaaacatc tcatgccaaa tttgaagagc taaaaaaagc attagattcg
      421 gacagccaag tactattagt tgtgattggt gagttttctc gtggtaaatc tagcttggtt
      481 aatgctttat taggaattca tttactgcgc tcagcacaag aggctactac tgcaattaac
      541 acctttgtaa aagcaatacc cgaaggacgt aatgagcgtt tcattcgcat tcattatcaa
      601 gatggtcgta cacctgatga aattacatgg gacagtgatg acgttttaga aaaatgggga
      661 actgaattaa atagtgacca cgctgatgct cgaaagcttg tcgattatat cgaagtgttt
      721 acctcgcatc ctttattaga taaaggttta gttcttattg atactccagg tttacaatct
      781 gtggtgcagc atcacgaaga aattactcgc cgtgcgattg ccgaagcaca tattgcgatt
      841 tgggtacaga gtaccgcgca attgggagga aatgccactg agtggcagtt tttatctcaa
      901 acaattcgta aaaactttcg taaatttatc actgtgataa atatgtggga tgcggtgtta
      961 gagccaacag actatcacga acaaaaaaaa tcagaagatg ttcgtgtgta tgaaaaatta
     1021 gagcgagtga gacaaaattt tagaaaaaat ctatttggta ttgcagaaga tgaattagaa
     1081 gtattaacga accctgagca tttaatgggg gtgagtgctt tatggcagtt aagtcacgac
     1141 ccacaaaagc aagcacgttc tggcgttgac aaactcgcgg aaagaattag cgtcatgttt
     1201 agttcgggtg aagcattaga gcaaatatat ttaaagcccc tgcaacagtt aagtcatatt
     1261 caggaacagc ttataggcag tattgttgat gagctggagc agttagcaag taatgacaca
     1321 ttagaagaac gtcaacgcgc tgccgataat ttgagtaagg acattaaaat tttagaacag
     1381 gatatgcaac aagaaaacaa tagtgctaaa caagagcata ataatgccgc taaggtatta
     1441 gcctcgcaaa taaaagacca actcatacaa cctcttaaat acctaaaaca agacattgag
     1501 acacaggttg atagaagcta tatcgaaaaa ttgctgcaac aaaagaaggc aaaaattagt
     1561 ttgccacccg aattggatag cgagtttgag gcagtgggca agcaagtggc acacagttgg
     1621 gagctacaaa agaaccaatt aggcaaaac
//
LOCUS       DAOQMB010000518         1649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56001_length_1649_cov_6.471142, whole genome shotgun sequence.
ACCESSION   DAOQMB010000518 DAOQMB010000000
VERSION     DAOQMB010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56001_length_1649_cov_6.471142"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..>1649)
                     /locus_tag="PLY05_11660"
     CDS             complement(73..>1649)
                     /locus_tag="PLY05_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03995.1"
                     /translation="ENDILLAYSVTTQSTTKTMEKLAAAVTASEIAAAPIGQTVKQVL
                     ESVGQVAPPNADQTNVYVGTLKVPYYNEASSAEKPTAILSSYWMADANQPDNTSSLLG
                     KIPCGLFAAKQVLNGIALEPSSSTTACFPLPVKQSDQTIPMIVTVPNGAAPDTGWPVV
                     IFQHGITRNRTDVFAVASTFSAAGFVTVAIDLPLHGLPQDNPFYKNLILEKVIEATGN
                     TALRAFETNNERTFDADFVNNATGAAGADKTADGSGTHFINLASPITTRDNLRQGASD
                     ILVLAKSLSNLNLTNLSGQPIKINGTQVRYASISLGSIVGTVALGADKSASLIGAASL
                     SVGSGGLPKLLDGSKSYGPIITAGLKGRDVLEGTDNYESFMRLAQTLTDSGDPINFAM
                     NTKMNRPIHFTQVLNDLVVSNDSIKGPTSATQDFVGATGFLSGNTALARAMGFSDKKE
                     IDIATVTKQNLTGSSWLQFNQGTHGSLINPAAPTGDTATDAEKATFLSITTEMQCQTV
                     NFLATGGAVVPVGCSK"
BASE COUNT          510 a          342 c          311 g          486 t
ORIGIN      
        1 tattaatgtg atttcgcttg ataaaaaaac aaaagaggca aaaaatgcct cttttgttat
       61 tggttttgaa agttatttag aacaacctac tggaacaact gccccacccg tcgccaagaa
      121 gttcactgtt tgacattgca tttctgtagt aattgataag aatgtcgctt tttcagcatc
      181 tgttgcagta tcacctgttg gtgcagcagg gttaatcaaa gagccatgtg taccttgatt
      241 aaactgcagc caagaactac ctgttaaatt ttgcttagta acagtagcaa tatcaatctc
      301 ttttttatca ctaaagccca ttgcacgtgc taaagcggta ttacccgata aaaagcctgt
      361 tgcgcctaca aaatcttggg tagcagaagt tggcccttta attgaatcat tagataccac
      421 taagtcattc aacacttgag taaaatgaat gggacgattc attttggtat tcatagcaaa
      481 gtttattgga tcaccactgt cagttaaggt ttgagcaaga cgcataaatg actcataatt
      541 atcagtacct tcaagtacat ctctaccttt taaacctgcg gtaataattg gaccatacga
      601 tttagagcca tctaacaatt taggcaagcc accactaccg accgataaag aagctgcacc
      661 tatcaatgaa gctgacttat ctgcacctaa ggctactgtg ccaacaatac tgcccaaaga
      721 aatactggca taacgcactt gagtgccatt aattttaata ggctgaccac tcaaattggt
      781 taaatttaaa ttagataaac ttttggccaa cactaaaata tccgaagcac cttgacgcaa
      841 gttatcacgc gtggtgatag ggctagctaa attaataaaa tgcgtaccag agccatcggc
      901 tgttttatct gctcctgctg cacctgtggc attattaaca aaatctgcat caaaagtgcg
      961 ttcattattg gtttcaaagg ctcgaagcgc ggtattacca gtggcctcaa taactttttc
     1021 taatattaag tttttgtaaa aaggattatc ttgaggcaag ccatgcaagg gtaaatcaat
     1081 tgcaactgtc acaaaacctg cggcagaaaa ggttgaagcc acggcaaaaa catcggtacg
     1141 gttacgagta atcccatgtt gaaaaatcac gacaggccag cctgtatctg gtgctgcacc
     1201 attaggcacc gttacaatca taggaatcgt ttgatcgctt tgtttaacag gcaatgggaa
     1261 acaagctgta gtactagaac ttggttcaag tgcaatacca ttcaagactt gtttagcagc
     1321 aaacaatcca caaggtattt tacccaataa gctacttgta ttatcaggtt gatttgcatc
     1381 agccatccaa taactactta aaatggctgt tggcttttct gccgaagaag cctcattata
     1441 ataaggcact tttaatgtac ctacatacac attggtttgg tcggcattag gaggtgcaac
     1501 ttgtcctaca gattctaaaa cttgtttaac ggtttgacca atcggtgcag ctgcaatctc
     1561 actagctgtt accgctgccg ctaatttttc cattgttttt gtggtgcttt gagtggttac
     1621 actataagcc aacaaaatat cattttcag
//
LOCUS       DAOQMB010000519         1648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56057_length_1648_cov_10.033898, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000519 DAOQMB010000000
VERSION     DAOQMB010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56057_length_1648_cov_10.033898"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1648
                     /locus_tag="PLY05_11665"
     CDS             <1..>1648
                     /locus_tag="PLY05_11665"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC03996.1"
                     /translation="RVSRVRGRLPDTIDEPVITKTEADAQPIIWLAFSSQQHTSLQIN
                     QFADKQVKSRLQTLDGVADIRIFGERKPAMRIWLDKEKLARFSLTPQDIESALRAQNV
                     EIPSGRVESVQREFSVLAETDLNTVEQFENIIIKQQDGLLLRIADVARVEIAPEDVRR
                     IARFNGTPAVALGVIKTSTANPLEVSKAVRAELPRIEQSLPKGMQVRLAYDSSVFIQE
                     SINSVQRTIIEAVVLVVLVIFFFLRSLRATLIPVITIPVSLIGALIFMQILGFSINTL
                     TLLAMVLAIGLVVDDAIVVLENIYRHIEEGKTPMQAAVQGSKEIGFAVLAMTFTLAAV
                     FAPIAFTEGRTGKLFAEFALSLAAAVIVSGFCALTLSPMMSSRLLKAHSGQENIVYRL
                     IEQFLQALTRGYQKSLTVVLKLRWLMVVVALGASGYMWLVFKALPSELSPTEDRGVIV
                     SIGIGPEGSTPTYFDKYAQQVEGIYSKVPERASTFLIIGFPDETKFISFNRFKPWEER
                     QRTTQQIVPTLMPQLSQVAGVMALATLPPSLGQGASSSPI"
BASE COUNT          444 a          305 c          369 g          530 t
ORIGIN      
        1 atcgtgtgtc tcgggtacga ggccgtttgc ctgataccat tgatgaacca gttattacta
       61 aaacagaagc cgatgcacaa ccaattattt ggctggcttt ttcttcacaa caacatacct
      121 ccttacaaat caatcaattt gctgataaac aagttaaaag tcgtttgcaa actcttgatg
      181 gtgttgccga tattcgtatt tttggtgaac gtaaaccagc gatgcgcatt tggttagata
      241 aagaaaaatt agcacgtttt agcctaactc ctcaagatat tgagagtgct ttacgtgcgc
      301 agaatgtcga aattccttct ggccgcgttg agagtgtaca gcgtgaattt tcggtattag
      361 cagaaacgga tttaaatacc gttgagcaat ttgaaaatat tattatcaaa caacaagacg
      421 gcttgttgtt acgtattgct gatgttgcac gggtagagat tgcgcctgaa gatgtgcggc
      481 gtattgcccg ttttaatggt acacctgctg tcgctttagg ggtgattaaa acctcaacgg
      541 ccaatccttt agaggtttcc aaagcggtac gtgcagagtt gccgcgtatt gagcaaagtt
      601 tgccaaaagg catgcaagta cgtttagcct atgattcttc agtctttatt caagaatcga
      661 ttaactctgt acaaagaacc attattgaag cggttgtttt agtggttttg gtgatttttt
      721 tctttttaag aagtttacgt gccaccttaa ttcctgtgat taccattcct gtgtcattga
      781 ttggtgcttt gatttttatg caaatcttag gcttttcaat taatacctta actttattag
      841 ccatggtgtt ggcgattggt ttggtggtgg atgatgccat tgtcgtgtta gaaaacattt
      901 atcgtcatat tgaagaaggc aaaacgccga tgcaagcagc agtgcaaggt tctaaggaaa
      961 ttggttttgc agtattagcg atgaccttta ccttagcagc ggtatttgcc ccgattgcct
     1021 ttaccgaagg acgaacagga aagttgtttg ctgagtttgc tttaagtttg gcagctgcgg
     1081 tgattgtctc aggtttttgt gcgttaactt tatcgccaat gatgagctca agattattaa
     1141 aagcgcattc aggccaagaa aatatagtct atcgtttaat tgaacaattt ttacaggctt
     1201 taactcgtgg ttatcaaaag tccttaactg tggttttaaa attgcgttgg ttaatggtgg
     1261 tagtggcgtt aggggcgagt ggctatatgt ggttggtgtt taaagcctta ccctcagaat
     1321 tatcacctac tgaagacaga ggcgtcattg tgtcaattgg tattggccca gaaggttcaa
     1381 cacctaccta ttttgataaa tatgcccaac aagtagaagg tatttatagc aaagtgcctg
     1441 aacgagcctc taccttttta attattggtt ttcctgatga aactaaattt attagtttta
     1501 atcgttttaa accatgggaa gagcggcagc gcactactca acaaatcgtc ccaaccttaa
     1561 tgccacaact gagccaagtg gcaggtgtta tggccttggc aacattacca ccgtctttag
     1621 gacaaggtgc gtccagttcc ccaattga
//
LOCUS       DAOQMB010000520         1645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56227_length_1645_cov_12.793082, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000520 DAOQMB010000000
VERSION     DAOQMB010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56227_length_1645_cov_12.793082"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="PLY05_11670"
     CDS             <1..774
                     /locus_tag="PLY05_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNC03997.1"
                     /translation="AIVLSLLLLIGVIVWQYDNFVSWVASKIPVSTEQQLGNAVLEKI
                     KAEGDLLESGKTVETISEIGNKLTQGSRYKYQWYVLKNDTVNAFAVPSGIIVVHSALI
                     EKAENADELAAVLAHEVQHVEQRHSLKHMINSVGWAAVLTIVLGDASAITAVIAHQLG
                     TSYFSRDLESEADKLGYATLVKAKIKPDGMASFLQKLADLSGDTEQGLGWISSHPETL
                     ERVNTTKQLLKDQPCQVCQPLAIDWQMVQNTLPKVDKAK"
     gene            complement(832..1554)
                     /locus_tag="PLY05_11675"
     CDS             complement(832..1554)
                     /locus_tag="PLY05_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC03998.1"
                     /translation="MKNHKTFLQTSLLCLLLAATTSYAAKNDDDDDPTEYLPYNEKAT
                     KEWSLIKDDKRHKIKAFSKKEDGYRFRSFKVQSVLNGSLEAAARCQFDIEGIPSWYMN
                     VTEAKLLKRVSDTEYYFYVKMPFGVPHRDVVVHAKVQPYSTKTGFLAISYRAAPDFIP
                     TKPGVIRMPAYETTTKFKPISEDQTEEQLEGYVDPGGEGLPIWLVNYAQRRTPYSNMI
                     GRQRLISKCAASDSPIPFKYKE"
BASE COUNT          496 a          292 c          351 g          506 t
ORIGIN      
        1 gccattgtat taagtttgct attgctgatt ggggtgatag tttggcaata cgataatttt
       61 gtctcgtggg tggcgagtaa aattcctgtg agtaccgagc aacaactggg taatgcagtc
      121 ttagaaaaaa taaaagctga gggagattta ttagaatcgg gtaaaacggt tgaaacgatc
      181 agtgaaatag gtaataagtt aactcaaggc tctcgttata aatatcaatg gtatgtgtta
      241 aaaaatgata cggtgaatgc ttttgctgtc ccaagtggca ttattgtcgt tcattcggca
      301 ttaattgaaa aagccgaaaa cgccgatgaa ttagcggcag ttttggctca cgaagttcag
      361 catgtagaac aacgccattc tttaaaacac atgattaata gcgtcggttg ggcagcagtg
      421 ttaaccattg tcttgggtga tgccagtgcg attaccgccg tcatcgctca ccagttaggc
      481 acaagttatt ttagccgcga tttagaaagt gaagcagata aattaggtta tgcaacgctt
      541 gttaaagcca aaattaagcc cgatggtatg gcgagctttt tacaaaagtt agctgattta
      601 tcgggcgata ccgaacaagg tttgggttgg atttcctctc atcccgaaac tttagaacgt
      661 gttaatacca ctaagcagtt attaaaagat caaccctgtc aggtttgtca gcctttggca
      721 attgattggc aaatggtaca aaataccttg ccaaaagtgg ataaagctaa ataattacaa
      781 cgataaaacc taccacaaag taggttttat catcgcattt ttaataatca attactcttt
      841 atacttaaat ggaatagggc tatcactggc tgcacactta gagataagac gttgtcgacc
      901 aatcatattt gaatagggag ttcgacgttg ggcatagtta actagccaaa ttggcagacc
      961 ttccccaccg ggatcaacat aaccttctaa ttgctcctcg gtttggtctt cgctaattgg
     1021 tttgaactta gtcgtggttt cataagcagg catacgaatg acacctggtt tagttggaat
     1081 aaaatcaggt gcagcacgat aagaaattgc taaaaagcca gttttagtag aataaggctg
     1141 aactttggca tgaaccacga catcacgatg tgggacacca aagggcattt tgacataaaa
     1201 atagtattcg gtatcactga ctcgttttag cagctttgct tcggtcacat tcatatacca
     1261 actgggtatt ccttcaatat caaattggca gcgtgctgcg gcctctagag aaccatttaa
     1321 aacactttgt accttaaacg aacgaaaacg atagccatct tcttttttag aaaaagcttt
     1381 gattttatgc cgtttatcat ccttgattaa agaccattcc ttagtcgcct tttcgttata
     1441 gggaaggtac tctgtggggt catcatcatc gtcatttttt gcggcgtagc tggtggtggc
     1501 agctaacaac aagcacagta agcttgtttg taaaaatgtt ttgtggtttt tcatttttat
     1561 actctgactt tttatatttg gtggtagttt taaaataaga caagtaaata ctagttatga
     1621 ctaagtttta tagtaaagct aattt
//
LOCUS       DAOQMB010000521         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56428_length_1642_cov_15.370510, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000521 DAOQMB010000000
VERSION     DAOQMB010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56428_length_1642_cov_15.370510"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLY05_11680"
     CDS             complement(<1..989)
                     /locus_tag="PLY05_11680"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNC03999.1"
                     /translation="MTRVWYNKSFSFVYNIVQLIKHQDVAKQFYLIVSHTQAHARGLL
                     VADESYVEPSGLVAKAYVDWCLAFCQQHKIDIFVVGKEAETIAQYQDLFRQQGVQVVL
                     VADSDTLKLLEDKSRFAAHLPTEIAVLPETVTIQNLAQFEQAYQDLRSRHDCLSIKPA
                     VSIFGLGFRKIDEKGDCLQHILSGEEYIVGLKELRQAMLEQNSFGKLLLMEFLDGDEW
                     SVDCLAIHGQLYAAVQRRKFMAQKGVPRQYIDNNPEIAMMCQQLTAHFKLNAQFNIQF
                     RAGKNGIRLLEINARPSGGMAMSCVAGVNLPYLLLRSCLDSSLPPVTINVGQYV"
     gene            complement(1051..1632)
                     /locus_tag="PLY05_11685"
     CDS             complement(1051..1632)
                     /locus_tag="PLY05_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HNC04000.1"
                     /translation="MAVNLQKGQKISLEKEAGTGLTRVIMGLGWDVAKKKGFFGFGGG
                     SAEIDLDSSCLMFDEAGQMVDIVWFRQLQSKDTSIKHSGDNRTGAGEGDDEQISVDLS
                     RVPAQVKSLVFTVNSFTGQSFSQVENAFCRLINAANNQEIARYNLSCQGDHSAQIMAK
                     LYKHNGEWKMHAIGENGQGRTFEQLMPQIRSYL"
BASE COUNT          529 a          383 c          237 g          493 t
ORIGIN      
        1 acatattgac ccacattgat ggtcactggt ggcaaacttg agtctagaca tgaccgcaat
       61 aacagatatg gcaaatttac acctgcaaca caagacatcg ccatacctcc agaaggtctt
      121 gcattaattt ctaacaatct aatgccattt ttacctgctc taaattgaat attaaactga
      181 gcatttaatt taaaatgtgc cgttagctgt tggcacatca tggcaatttc aggattatta
      241 tcaatatatt ggcgtggcac tcctttttgt gccataaact tacgccgttg taccgctgca
      301 tataactgtc catgaatagc taaacaatcc acactccatt catcaccatc caaaaactcc
      361 attaacaaca atttaccaaa gctattttgc tcaagcattg cctgccgtaa ctcttttaaa
      421 ccaacaatat attcttcacc acttaaaata tgctgcaaac aatcgccttt ttcatcaatt
      481 tttctaaaac ctaagccaaa aatagaaaca gcaggtttaa tagacaaaca atcatgacgg
      541 ctgcgtaaat cttgatatgc ttgctcaaat tgcgctaagt tttgtatggt caccgtttcg
      601 ggcaacacgg caatttctgt tggtaaatgt gcggcaaaac gacttttatc ttccaacaat
      661 tttaaggtgt cactatctgc caccaataca acttgtacac cctgctgacg aaataagtct
      721 tgatattgtg caatcgtttc tgcttctttg cccaccacaa aaatatcaat cttatgttgc
      781 tgacaaaagg ctaaacacca atcaacatag gctttagcca ccaaaccact tggctcaaca
      841 taactttcgt ctgctaccaa caaaccacgt gcatgtgctt gagtatgact aacgattaaa
      901 taaaactgtt tggcgacatc ctgatgttta attaattgaa caatgttata cacaaaagaa
      961 aacgacttgt tataccagac acgggtcata aaaattctct aaaaaaaaga ggcggaaagc
     1021 ccgcctcttt tagggtctat taactgacgc ttataaataa ctgcgaattt gtggcattaa
     1081 ttgttcaaag gtacgacctt gaccattttc accaatcgca tgcattttcc actcaccatt
     1141 atgtttatac aatttcgcca taatttgtgc agaatgatcg ccttggcaag ataagttata
     1201 acgcgcaatt tcttgattgt ttgccgcatt aattaaacga caaaaggcat tttcaacttg
     1261 tgaaaagctt tggcctgtaa aactattaac ggtaaaaacc aaacttttta cctgtgcagg
     1321 cacacgactt aaatcaacac tgatttgttc gtcatcacct tcacctgcac ccgtacgatt
     1381 atcaccacta tgtttgatgc tagtatcttt gctttgtaat tgtctaaacc aaacaatatc
     1441 caccatttga ccagcttcat caaacattaa acaagaagag tccaaatcaa tttcggcaga
     1501 accaccacca aaaccaaaaa aacctttctt tttggcaaca tcccagccta atcccataat
     1561 cacacgagtt aaaccagtac ctgcttcttt ttctaaagaa attttttgcc ctttttgtaa
     1621 attaacggcc atttttactc tc
//
LOCUS       DAOQMB010000522         1642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56446_length_1642_cov_4.425961, whole genome shotgun sequence.
ACCESSION   DAOQMB010000522 DAOQMB010000000
VERSION     DAOQMB010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56446_length_1642_cov_4.425961"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="PLY05_11690"
     CDS             <1..545
                     /locus_tag="PLY05_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04001.1"
                     /translation="EAMNRQDFEWVLNINFWGVVNGCESFLPQLRQQKDAHIVNISSI
                     FGIIGVPSQSAYNASKFAVKGFTEALRQELNGSNIHVTCVHPGGIKTNIARNARVHND
                     MFGKAADPKKLAADFDKLAATTAEEAARQIVRAIETNQKRLLIGKDAKALDAIQRLLP
                     NHYDTVLGVGYKLMNKLAKR"
     gene            575..1120
                     /locus_tag="PLY05_11695"
     CDS             575..1120
                     /locus_tag="PLY05_11695"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HNC04002.1"
                     /translation="MKGIVFTEFLEMVEDRFSLDMVDDLIDDCDLPSGGVYTAVGTYS
                     HEEIVALVSALSQRSSIPISQLLKVFGEHLFGQFAKNYSVFFANVTNALDFLHGIEDV
                     IHAEVKKLYPDAQLPRFYTEEYQANKLVLIYQSNRHFEDLAEGLMIGCIKYFNEKINI
                     TREAIYNKDIKQERFTLVRYE"
     gene            1126..>1642
                     /locus_tag="PLY05_11700"
     CDS             1126..>1642
                     /locus_tag="PLY05_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNC04003.1"
                     /translation="MIDEVRWQKRLERERAARKAAELLLEEKSLQLYKSNQVLQDLAT
                     QLEQKVSVRTKELQIALSKAEAATKAKSDFLAVMSHEIRTPLNGIIGMTQLLAMTHLS
                     DEQREYLSTVASSSDSLLILINDILDFSKIEAGKLELEHRSFNLLHEIKSIFQLYQVV
                     AQQQGLHFTLEI"
BASE COUNT          529 a          295 c          314 g          504 t
ORIGIN      
        1 tcgaagcgat gaaccgtcaa gattttgaat gggtattaaa tatcaatttt tggggtgtgg
       61 taaatggttg tgaatcgttt ttgccccaat tacgccaaca aaaagacgca catattgtta
      121 atatttccag tatttttggc attattggtg tgccttccca atctgcttat aatgccagta
      181 agtttgcagt aaagggtttt accgaagcct tacgccaaga attaaacggc agcaacattc
      241 atgtaacttg tgtgcatcct ggtggcatta aaaccaatat tgcccgtaat gctcgggtgc
      301 ataatgatat gtttggcaaa gcggctgacc ccaaaaagct cgcggctgac tttgataaat
      361 tagcggcaac caccgctgaa gaagctgcca gacaaattgt tcgtgctata gaaactaacc
      421 aaaaacgttt gttaattggc aaagatgcca aagccttaga tgcgattcaa cgtttattac
      481 cgaatcatta cgacaccgtt ttgggtgtag gctataagct aatgaataaa ctggctaaac
      541 gctaaatctg tcaacataaa gagacaagaa caccatgaaa ggcattgttt ttaccgaatt
      601 tttagaaatg gttgaagatc gtttttcttt agacatggtt gatgacttaa tagatgattg
      661 tgatttacct tcaggtggtg tttatactgc tgttggtacc tattcgcatg aagaaattgt
      721 cgctcttgtc tctgctctat cacaacgcag ttccattcct atatcacaac ttttaaaggt
      781 gtttggcgaa catttatttg gtcagtttgc taaaaactat agcgttttct ttgctaacgt
      841 gaccaacgcc ttagattttt tacacggtat tgaagatgtg attcatgccg aagtcaaaaa
      901 gctttatcct gatgcacaac tgccacgttt ttataccgaa gaatatcaag ccaataaact
      961 ggtgttaatt taccaatcta atcgacattt tgaagactta gccgaaggtt taatgattgg
     1021 ttgtattaag tattttaacg aaaaaattaa cattactcgc gaagctattt ataataaaga
     1081 tattaaacag gagcgtttta ccctagtacg ctacgagtaa taataatgat tgatgaagta
     1141 cgttggcaaa aacgtttaga gcgtgaaaga gctgctcgca aagcagccga attgttatta
     1201 gaagaaaaaa gccttcagct atataaatcc aaccaagtgc tacaagattt ggcaactcaa
     1261 ttagaacaaa aagtctcagt tcgtactaaa gagttacaaa ttgctttaag caaagctgaa
     1321 gcagcgacta aagccaaaag tgattttttg gcggtaatga gtcatgaaat tcgcacacca
     1381 ttaaatggca ttattggtat gactcaacta ttagcgatga cgcatctttc tgatgaacaa
     1441 cgcgaatact tgagcacagt tgcaagtagt agtgactctt tattaatact gattaatgac
     1501 attttagatt tctcaaaaat tgaggctggt aaattagaat tagaacatcg ttcttttaat
     1561 ttacttcacg aaattaaatc catttttcaa ctctaccaag ttgtagccca acaacaagga
     1621 ctacatttta cccttgagat ta
//
LOCUS       DAOQMB010000523         1640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56578_length_1640_cov_5.930599, whole genome shotgun sequence.
ACCESSION   DAOQMB010000523 DAOQMB010000000
VERSION     DAOQMB010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56578_length_1640_cov_5.930599"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLY05_11705"
     CDS             complement(<1..416)
                     /locus_tag="PLY05_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNC04004.1"
                     /translation="MARYKIVHVMATAGGVVGGLEKHTLELCAALAQQHEVHLLADRP
                     YQGVCHKQIHFHPINFSLSRWNPFLYWQIAQTINHIQPNIVHAQAGKAASIIRWLKWL
                     FKQVKFVATVHGTKKDITAYAAMDGVITVSEQLAEA"
     gene            complement(398..1315)
                     /locus_tag="PLY05_11710"
     CDS             complement(398..1315)
                     /locus_tag="PLY05_11710"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNC04005.1"
                     /translation="MSLISPLIIWQFVDGKAGHEQQSTGLIKALEKLYPVQSIKIDIR
                     QHPVGVVDWILKRLPDYPLPHYIIGAGHRTHLALLAAKRITKAKTIVLMKPSLPISWF
                     DYALIPEHDKPTPSPKVLVTHGVLNTIEPSTQQQANKGLMLLGGASKRHGWDEISLVK
                     QMLLVLDATPHIQWQISTSRRTPEHTLLEVRNIAPHAKVWAANETPQGWVAQQLQQAS
                     QVWVTEDSISMIYESLTAGAQVGILAVPRLQKDRITQAIDDLAAQGQLVLFKQWQQSQ
                     QLANKQSFCEAQRAAQWLLEQEGLWLATK"
BASE COUNT          469 a          367 c          319 g          485 t
ORIGIN      
        1 aaggcctctg ccaactgctc acttaccgta atcacaccat ccattgctgc atatgcagta
       61 atatcttttt tggtgccatg cactgttgcg acaaatttaa cttgtttaaa caaccacttt
      121 aaccaacgaa taatgctcgc agccttacct gcctgagcat gaacaatatt gggttgaata
      181 tgattaattg tttgtgcaat ttgccaatat aaaaagggat tccatcgact caaactaaaa
      241 ttgattggat gaaaatgaat ttgcttatgg caaacaccct gatagggacg atctgccaat
      301 aaatgcactt catgctgctg tgctaatgca gcacaaagct ctagggtgtg cttttctaag
      361 ccaccaacga cgccacctgc cgttgccatg acatggacta ttttgtagcg agccatagcc
      421 cttcttgctc caacaaccat tgtgcagcac gttgcgcctc acaaaaagat tgtttattgg
      481 ccagttgttg actttgttgc cattgtttaa atagcactaa ctgcccttgc gcagctaagt
      541 catcaattgc ctgtgtgatg cgatcttttt gtaaacgagg aacagccaag ataccaacct
      601 gtgcccctgc tgtcaggctt tcataaatca tcgaaatgct gtcttctgta acccacactt
      661 gtgaggcttg ttgtagttgt tgagccaccc aaccttgtgg agtttcatta gctgcccaga
      721 ctttggcgtg tggtgcaatg ttacgcactt ctaataaggt atgttcaggg gtacgtcgag
      781 aggtcgaaat ttgccattga atatgcggcg tagcatctaa taccaacaac atttgtttga
      841 ctaaagaaat ctcatcccaa ccatgccgtt tggacgcacc tcccaacaac attaaccctt
      901 tgtttgcttg ttgttgtgtg cttggctcaa tggtgtttaa tacaccatgt gtcactaaga
      961 ctttggggct gggtgttggc ttatcatgct caggaattaa ggcataatca aaccaagaaa
     1021 taggcaaaga tggcttcatt aacacaattg ttttggcttt ggtaatccgt tttgccgcta
     1081 ataatgctaa atgggtacga tggcctgcgc caataatata atgaggcaaa ggataatcag
     1141 gtaaacgttt taaaatccaa tcaacaacac ccacaggatg ctgacgaata tcaattttaa
     1201 tgctttgtac tggatagagc ttttctaagg ctttaattaa gccagtgctt tgttgctcat
     1261 gccctgcttt gccatcaaca aattgccaaa taatcagtgg ggaaattaga gacatgacat
     1321 cctacgcctt aaaaacacct aacattagcc tagccctctc ctaaaagaga tgagtatgta
     1381 gacaagtgtg aaatttgttt ttagcgataa agctccctct cctactcgga gagggtgatt
     1441 ttcggcctaa tcatgcgtta attacgcctc ctttagacct caccctagcc ctctcctaaa
     1501 aggagaggga acaccatcgc tatatccaaa aactgtgtaa atacttattc cctttagaaa
     1561 gagaaacaat cattagttga caaaagttaa ccaatgttta tagctttcat ttttgccatt
     1621 cactgtatca aagaataact
//
LOCUS       DAOQMB010000524         1637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_56798_length_1637_cov_3.411504, whole genome shotgun sequence.
ACCESSION   DAOQMB010000524 DAOQMB010000000
VERSION     DAOQMB010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56798_length_1637_cov_3.411504"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..>1637)
                     /locus_tag="PLY05_11715"
     CDS             complement(275..>1637)
                     /locus_tag="PLY05_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC04006.1"
                     /translation="GRKIYVSCDKYGAVGDDNVADYANDKVGERSLYGLVFKNCHQFS
                     QQCVEQSQQQIDHSVLDTISNFVGGDTWEPTIGALKRAAHKQLGATKWRLWDWQNQQQ
                     QEATPEPDWQAQQDFFKSLPLNADSIQQIREQLSEAKAYEQEIADENIPQHIRKHLVS
                     FRKLMQDVSDTYEKAKGFLAACGGANFCYADLKNSTEDFSALANTMQNNAQIKELARK
                     MGRAYISEEKKKQTKIPQASKSEVHGTHRSDDVLRVLPSELVNLEDEELEMLFYARLL
                     EKQLLTYELQGTTHVAGETTETHQQRTGPVVACLDTSGSMQGAPMLKAKALLLAIANI
                     LKQENRSLHVLLFGASGEIREFAMVAKNEAAGLLQFLQQGFGGGTDFETPLQRALTII
                     KTEKDYQKADVLMISDGDCQLSEAFLQKFKTQKSILDCSIYSVLCSGSRVNDNFSDEV
                     VVL"
BASE COUNT          437 a          369 c          313 g          518 t
ORIGIN      
        1 gcccttctaa aaaaccatta gaaccaaagg caaatttacg tccgacagcg gtaaacactg
       61 ataataagac ggtggctaaa attagccatg ccaccacacg tcccaccgtt ttgttgagtg
      121 tgtcgatagc cgcacacact ttatttaatc cacgcataac cgtaaacaac tggagttgaa
      181 atggggcgaa tgatacgcgt ttggggcgag tttttgtact gatattattt tttaggtatg
      241 gctttagagt ctagcaagtg agagggtgct attgttatag caccacgact tcatcgctaa
      301 aattgtcgtt aacacgtgaa cccgaacata acacggaata aatactacaa tctaaaatac
      361 ttttttgtgt cttaaatttt tgcaaaaaag cctccgataa ttggcaatcg ccatcgctaa
      421 tcatcaaaac atcggctttt tggtaatctt tttctgtttt gataatcgtt aaagctcgtt
      481 gtaatggcgt ttcaaaatct gttccaccac caaacccttg ttgcaaaaat tgcaataagc
      541 ctgccgcttc gtttttggct accatagcaa attcacgaat ttcaccactt gccccaaata
      601 acagtacatg caatgagcga ttttcttgtt taagaatatt cgcaatcgcc aatagtaagg
      661 ctttggcttt aagcatcggt gcgccttgca tactgcccga agtatctaaa caggcgacca
      721 ctggccctgt gcgttgttga tgggtttcgg tagtttcgcc cgcaacgtgt gttgtgcctt
      781 gtaattcata ggtgagtaat tgcttttcga gtaaacgcgc ataaaacaac atttctaact
      841 cttcatcctc aagattgact aactcacttg gcaacactct cagcacatca tcactgcggt
      901 gtgtgccatg cacttcgctt ttactcgctt gaggaatttt ggtttgtttc tttttttctt
      961 cgctaatata ggcacgtccc atttttcgtg ccagttcttt aatttgtgca ttgttttgca
     1021 tggtgtttgc taaagccgaa aaatcctcag tgctgttttt taagtcggca taacaaaagt
     1081 tagctccccc acaagcagct aaaaagccct tggctttttc gtaggtatca ctgacatctt
     1141 gcatcagctt tctaaagctc actaagtgct tgcgaatatg ttgaggaata ttttcgtccg
     1201 caatttcttg ctcataggct ttggcttcgg acagttgctc acgaatttgt tgaatactat
     1261 cggcattaag gggtaagctt ttaaaaaaat cttgttgcgc ttgccaatca ggttcgggcg
     1321 ttgcttcttg ctgttgttgg ttttgccaat cccacaaccg ccactttgtt gcccctaact
     1381 gtttatgagc agcgcgttta agtgcgccaa tggttggctc ccatgtatca ccgccaacaa
     1441 aattgcttat tgtatctaat acagaatggt caatttgttg ctgactttgc tcaacacatt
     1501 gttgcgaaaa ctgatgacaa tttttaaaca ccaaaccata aagcgagcgt tcaccgactt
     1561 tatcattggc ataatcagcg acattatcat cacccactgc accatattta tcacaagaaa
     1621 cataaatttt gcgacct
//
LOCUS       DAOQMB010000525         1632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_57118_length_1632_cov_4.475587, whole genome shotgun sequence.
ACCESSION   DAOQMB010000525 DAOQMB010000000
VERSION     DAOQMB010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57118_length_1632_cov_4.475587"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PLY05_11720"
     CDS             <1..1298
                     /locus_tag="PLY05_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04007.1"
                     /translation="KADEIAKATTADANAKAAVTAHPDDVKKKEFILAKAQAVLDNAT
                     AAVQPAKDAKTLADNNKTDADKALEAINKAKAEADKAATSATATKAAADKLLASTNTA
                     ATSIKGTAADAQAAKDTTTTAQTKSQTAADKADASGEAAIAKVIADKNLVSKIAAQVA
                     ADKNLADAQGAFDSDSGYTEAGDTLGDKYLTQNTNFVESSGTFVEGVRKVTYKYKVRA
                     VSEDNVESDDSIEIEAKDNTKPKVDTITMNTASNVVTVVFNEPINEIDFRTQANYVLS
                     APANSGITLPALSNSLFKPDNKTVVLTYMANIPAGSIVTVSGVKDLAGNVLSEVGNKA
                     TVDKVTSSIAGFVGSTQVTVFFSADMDKDSAETAANYTWAAGVGPALLAANGVVYNAA
                     DNSVTLTYVSAIPFGATLNVSGVKDANGTVVESNTKAFN"
     gene            1470..>1632
                     /gene="sat"
                     /locus_tag="PLY05_11725"
     CDS             1470..>1632
                     /gene="sat"
                     /locus_tag="PLY05_11725"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF025664.2"
                     /note="ATP sulfurylase; ATPS; converts ATP and sulfate to
                     5'phosphosulfate and pyrophosphate; in some organisms this
                     enzyme is involved in the incorporation of inorganic
                     sulfate while in others it is involved in the production
                     of ATP in the reverse direction; the enzyme from Thermus
                     thermophilus is dimeric and binds a zinc ion that is
                     coordinated by cysteine and histidine residues that are
                     not found in all related proteins but is found in some
                     thermophilic organisms; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HNC04008.1"
                     /translation="MSLVKPHGSDVLLPLLLEGEALAAEQARAQSLKKVLISSRESGD
                     LIMLGIGGFT"
BASE COUNT          499 a          342 c          384 g          407 t
ORIGIN      
        1 gtaaagcgga tgaaattgcg aaggctacaa ctgctgatgc caatgctaag gcagcggtga
       61 cggctcatcc tgacgatgtt aagaaaaagg agtttatctt agcaaaagcg caagcagtac
      121 ttgataatgc tactgcagct gttcaacctg ctaaagatgc taaaacgctt gctgacaaca
      181 ataaaacaga cgctgataag gctttagagg caatcaataa ggccaaagca gaggctgata
      241 aagcggcaac cagcgcaaca gccaccaaag ccgctgctga taaattattg gctagcacaa
      301 acaccgcggc aacctctatt aagggaacgg cagctgatgc gcaagcagcc aaagatacaa
      361 cgaccacagc tcaaacaaag tcacaaactg cagcagataa agcggatgct tctggagaag
      421 cagccattgc taaagtgatt gccgacaaaa acttggtcag caaaatagca gcacaagtcg
      481 cggctgataa aaacttggca gacgcacaag gggcttttga ctcagactct ggctatactg
      541 aggcgggaga taccttgggc gataagtatc tgacacaaaa caccaacttt gttgaaagca
      601 gcggtacatt tgttgagggc gtgcgtaagg tgacttacaa gtacaaagtg cgtgccgtga
      661 gcgaagacaa cgtagagtcc gatgacagta tcgaaatcga agctaaagac aataccaagc
      721 ctaaagttga tactatcacc atgaacaccg cgtctaacgt agtaactgtg gtgtttaacg
      781 agccaatcaa tgaaattgac tttagaacac aagctaacta tgtgttgagt gcaccagcca
      841 atagcggcat tactctccca gcattgtcta acagcttgtt taaaccagac aacaaaaccg
      901 tggtattgac ctatatggct aacattcctg caggttcaat tgttacggtg tcgggtgtta
      961 aagacttagc aggtaatgtc ttaagcgaag taggcaataa agctactgtt gataaagtga
     1021 cctctagtat tgctggcttt gttggtagta cacaagtgac tgtcttcttt agcgcagaca
     1081 tggataaaga cagtgccgaa actgctgcaa attacacatg ggcggcaggg gttggtccag
     1141 cgttgttggc tgctaatggt gttgtttaca atgccgctga taatagtgtg accttaactt
     1201 atgtgtctgc tattcctttt ggtgctacct taaacgtgtc aggcgttaaa gatgctaacg
     1261 gtacagtggt tgaaagcaac accaaagcct ttaactaagc taatttaatg agtaaggttg
     1321 gctttagtca gccttgctta taaaaagaag cttgtttaaa actaaaagcg gtgtgagtga
     1381 aagctcatgc cgtttttgtt ttttatcgcg gccaaaattg cgtataatct ttgtcttttt
     1441 tacgctctgc aagatttaga ggaattttta tgtcattagt caaaccccac ggcagcgatg
     1501 ttctcttacc cttattatta gaaggcgaag cccttgccgc cgaacaagct cgcgcacaaa
     1561 gcctcaaaaa agtgcttatt agctctcgcg aaagcggtga cttaattatg ctcggtattg
     1621 gtggttttac cc
//
LOCUS       DAOQMB010000526         1628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_57367_length_1628_cov_20.045137, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000526 DAOQMB010000000
VERSION     DAOQMB010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57367_length_1628_cov_20.045137"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..626
                     /locus_tag="PLY05_11730"
     CDS             39..626
                     /locus_tag="PLY05_11730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNC04009.1"
                     /translation="MIRLLVVDDQELVRQGIVALLGFEQDLQVIGQAVDGEDCLAKLP
                     LLKPDVVLLDIRMPKRNGLDTLREMRLLGDFTPVILLTTFDEPITIAQGKQLGAQACL
                     LKDIAHEQLTAAIRTVAEGKTLASPNVPTHTVFSRRELAIIAQLCAGKQNKEIAQALQ
                     LSAGTVRNYLSIILEKLEVRDRTQAIIRLRELGLA"
     gene            768..1241
                     /locus_tag="PLY05_11735"
     CDS             768..1241
                     /locus_tag="PLY05_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04010.1"
                     /translation="MRLIVLVLSLVFTVFTQTAMAAVERLDMKLILDKNGIKIWSYRL
                     PDSPYYGFKAVTTVKSSLTGAVAMVMDTDSANRWLYRTLAVDPLKLENNNMDFVIRVV
                     SDFPWPFKDRESVVKGKVTQDPETHVVRIDSRSVDNHPVREGYERMPSVEGGVVI"
     gene            1201..1434
                     /locus_tag="PLY05_11740"
     CDS             1201..1434
                     /locus_tag="PLY05_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04011.1"
                     /translation="MNVCQALRGGWLFRPLANRQVEVTMTGHADLGGAVPAVIVNLLI
                     QEHPYNSLLGMKKLINESRFQTARLAGIKEPVM"
BASE COUNT          482 a          256 c          377 g          513 t
ORIGIN      
        1 tttagcgtca gtgtcttttt tgcagaggag aatttgtcat gattcgctta ttggtggttg
       61 atgaccaaga attagtgcgt cagggcattg ttgcgttatt aggctttgaa caagatttac
      121 aagtgattgg tcaagcggtt gatggtgaag attgtttggc caaattaccg ttactaaaac
      181 ctgatgtagt attgttagat attagaatgc ccaaacgtaa tggtttagat accttgcgtg
      241 aaatgcgctt attaggtgat tttacgccag ttattttgtt aactaccttt gatgagccaa
      301 ttaccattgc tcaaggtaaa caattaggcg cacaagcttg tttattaaag gatattgctc
      361 atgagcaatt aacggctgcg attagaacgg tggcagaagg taaaacgttg gctagtccta
      421 atgtgccaac acatactgtt tttagtcgcc gtgaattagc aattattgct cagttgtgtg
      481 ctggtaaaca aaataaagaa attgcacaag ccttgcaact tagtgcaggt acggtacgta
      541 attatttatc gattatttta gaaaagttag aagtgagaga tcgaactcaa gcgattattc
      601 gtttacgtga gttaggtttg gcttaagcat caaaaaaaat ggcattaaca gccaagtgtt
      661 tgcctataga aattaattac actatttagt atgatgtttg tcactgtggt tttggtcact
      721 tgctatttat tattttagct aagctggccg atattctgga gaatagtgtg agactaatcg
      781 tattggtgtt aagccttgtt tttactgtat ttacacaaac agcaatggca gcagtagagc
      841 gactagatat gaaactcatt ttagataaaa atggcattaa aatttggtct tatcgtttgc
      901 cagatagtcc atattatggt tttaaagcag tcaccaccgt aaaaagcagc ttaacaggtg
      961 cggtagcaat ggtgatggat acggattcag cgaatcgctg gttgtatcgt acattggctg
     1021 tagatccgct taaattagag aataacaaca tggattttgt gattcgagta gtgagtgatt
     1081 ttccttggcc atttaaagat cgtgaatctg ttgtcaaagg taaagtgaca caagaccccg
     1141 aaacacatgt ggttagaatt gatagtcgca gtgttgacaa tcatcctgtc cgtgaaggtt
     1201 atgaacgtat gccaagcgtt gagggggggg tggttattta gacctttagc caatcgccaa
     1261 gttgaagtga cgatgacagg tcatgctgat ttaggtggcg cagtccccgc agtgattgta
     1321 aatttgttga ttcaagaaca tccctataat tcattgttgg gtatgaaaaa attgataaac
     1381 gagtcgcgct ttcaaaccgc tcgtttagca ggcatcaaag agcctgtgat gtaacaacat
     1441 aaaaaaaggc tggtttaacc agcctttttt attttagggg atagtcagct tattgattag
     1501 tattaggact tacgcattga caggttaagg attttaataa ttgctgtaag cttgtcaaat
     1561 gataagacaa accacaagac caccaacggc aaggtgtaca ctgccgatgt atatgggttt
     1621 cttaatga
//
LOCUS       DAOQMB010000527         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_57697_length_1623_cov_14.193878, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000527 DAOQMB010000000
VERSION     DAOQMB010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57697_length_1623_cov_14.193878"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLY05_11745"
     CDS             complement(<1..319)
                     /locus_tag="PLY05_11745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC04012.1"
                     /translation="MAAYYYKYRVLSKDFVDGYLFQQPLKTRDTMSLRYQIIPVTPFE
                     QNCTLLWCDETMEGTLIDAGGDIAKLHAAIDKNNIKITKLLLTHGHLDHVGAAKQVAQ
                     HYGV"
     gene            356..844
                     /locus_tag="PLY05_11750"
     CDS             356..844
                     /locus_tag="PLY05_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HNC04013.1"
                     /translation="MPSFDVVSELEMFEVNHAVTNTNKEIQTRFDFRGSNVKVEINEK
                     NKEIKIIGESDFQVEQVYAMLELHCLKRKIDAQCLDPQKMAASGKEIHQVVKLKDGLD
                     SDTAKKMVKIIKDSGIKAQASIQGEKVRVTDKKKDTLQAVMNLLREQKLGLPLQFNNF
                     KD"
     gene            847..1326
                     /locus_tag="PLY05_11755"
     CDS             847..1326
                     /locus_tag="PLY05_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004655924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNC04014.1"
                     /translation="MSSIKRVVESSKWLPANIRATVISKIFGKVVPYVGTSGLLYEEI
                     TPERVVVSIKNQRKVQNHINNVHAAAMALLAETATGFVVGMNLPDDKLPLIKSLKVSY
                     YKRTQGDMRAVATLSAEDIARIEREPKGEVLVPVIVTDESGGEPIKCEMLWAWVSKR"
     gene            complement(1358..>1623)
                     /locus_tag="PLY05_11760"
     CDS             complement(1358..>1623)
                     /locus_tag="PLY05_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04015.1"
                     /translation="VSPEKAGQYTMPVRKMISEVIPIMAVFVILVLVSALSSSILPPT
                     KWMWGVVLLFSLLIPLLWSRFVKLHSRLQIALIETLDDDSKAH"
BASE COUNT          527 a          296 c          317 g          483 t
ORIGIN      
        1 taacaccata atgttgagca acttgttttg ctgctcctac atggtctaaa tggccgtgtg
       61 ttaataatag cttggtaatt ttaatattat tcttatcaat cgcagcatgt agcttggcaa
      121 tatcgccacc tgcatcaatt agcgtccctt ccatcgtttc atcgcaccat aataaagtgc
      181 agttttgttc aaaaggcgtg actggaataa tttgataacg taaggacatg gtatctctcg
      241 tctttagggg ctgttggaac aaatatccgt caacaaagtc tttactcaat acacgatatt
      301 tgtagtagta tgctgccatt tttatagcag ctattcaatc tatggggaaa agcttatgcc
      361 ttcttttgat gttgtatctg aattagaaat gtttgaagtg aatcatgccg tgactaatac
      421 caacaaagaa attcaaactc gttttgattt tcgtggcagt aatgtcaagg ttgagattaa
      481 cgaaaaaaac aaagaaatca aaattattgg cgaatctgat tttcaggttg aacaagttta
      541 tgccatgttg gaattacatt gcctcaaacg taaaattgac gcgcaatgtt tagacccaca
      601 aaaaatggcc gcttctggca aagaaattca tcaagttgtt aaactcaaag atggtttgga
      661 tagtgatact gccaaaaaga tggtcaaaat tattaaagac tcaggtatta aggcacaagc
      721 cagtattcaa ggcgaaaaag ttcgtgtgac cgacaaaaag aaagacacct tacaggcggt
      781 gatgaatttg ctacgtgagc aaaaacttgg tttacctcta cagtttaata actttaagga
      841 ctaatcatgt ctagtatcaa gcgagttgtc gaatcttcta aatggttacc tgctaatatc
      901 cgcgctaccg ttatttccaa aatttttggc aaagttgtgc cttatgttgg cacttctggt
      961 ttattgtatg aagaaattac accagaacgt gttgttgtga gcatcaaaaa tcagcgtaaa
     1021 gttcaaaatc atattaataa tgttcatgct gcggcaatgg ctttattagc cgaaacagca
     1081 actggttttg tggtaggtat gaatttacca gatgataaat tacccttaat taaaagccta
     1141 aaagtgagct attacaagcg tacccaaggt gatatgcgtg cagtagccac attgtctgct
     1201 gaagatattg cacgtattga acgcgaacct aaaggcgaag ttttagtgcc tgttattgtc
     1261 actgatgaaa gtggtggcga acccattaaa tgcgaaatgt tatgggcttg ggtgtctaaa
     1321 cgttaaaaaa ttaaggggct aaatagcccc ttaattttta gtgcgcctta gagtcatcat
     1381 ctaaggtttc aatcaaagca atttgtaaac gcgaatgtag tttaacaaaa cgactccata
     1441 ataatggaat taacaagcta aataatagta ctacccccca catccatttg gttggcggta
     1501 aaatactact acttaaagcc gataccaaca ctaaaataac aaacaccgcc ataatcggaa
     1561 taacttcgga aatcatttta cggacaggca ttgtatattg cccagccttc tcagggctga
     1621 cac
//
LOCUS       DAOQMB010000528         1615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_58213_length_1615_cov_16.345513, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000528 DAOQMB010000000
VERSION     DAOQMB010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58213_length_1615_cov_16.345513"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(292..1050)
                     /locus_tag="PLY05_11765"
     CDS             complement(292..1050)
                     /locus_tag="PLY05_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005307172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNC04016.1"
                     /translation="MTSLTEIMQLLQTTLKPHLFSDYCPNGLQVEGRAEVKRLVTGVT
                     ACQALIDKAIDLQADAILVHHGFFWKSENPCVVGMKKNRLQALLKHDISLLAYHLPLD
                     AHAELGNNAQLAQKLGLNMAEALYPDNPKQVGNIAVLEQALSVDELSHRCQQVLGQAP
                     IHIAGQVQHLRRIAFCTGAAQGFIEQAVLMGCDAYISGEISEPTVHIAREMGIHYFAA
                     GHHATERYGVQAVGEFLAAKLGIEHIFVEVENPV"
     gene            1122..>1615
                     /locus_tag="PLY05_11770"
     CDS             1122..>1615
                     /locus_tag="PLY05_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004782756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNC04017.1"
                     /translation="MSTPYFRLIALSSLIALTACQPKDKAESSTPTQVTLSQERPLTI
                     QGSASGVSSYAPTVKATAPAVVNIFTTAIVKHNSHPLMNDPMFRRFFEFHGMNQNESE
                     PRSGLGSGVIVSKDGYILTNHHVVAKADEIIVALHDGRKAKAKVIGTDPDSDLAVIKI
                     DLKD"
BASE COUNT          494 a          363 c          283 g          475 t
ORIGIN      
        1 caacattcaa agattgtgtt tgtgcatgag caaaactaac catcaacaaa ctcagcaccg
       61 caacaatatt tttcaaaaat attctctctt acgcaaaata tgtgaatatg tattaataaa
      121 tacacatttt caaaccaatc tttcgattgt gccacttgat taacaaaagt acaatatttt
      181 tgaacaaata ataaacctat atggtagaaa ataaggacaa tcgctataga gaagtgactg
      241 gctatttgta tcgttgttaa gatttacagg cgcgaatttt gcgcctgttc ctcaaacagg
      301 attttccact tccacaaaaa tatgctcaat gcctaattta gccgccaaaa actcacctac
      361 agcctgcaca ccataacgct cggtggcatg atggcctgcg gcaaaataat gaatacccat
      421 ttcacgggca atatgcaccg ttggctcgga aatttcacca ctgatatagg catcacaccc
      481 cattaacacc gcttgctcaa taaaaccttg tgctgcaccc gtacaaaagg caatacgcct
      541 taaatgctgt acttgtcctg caatatgtat tggcgcctga cctaatacct gttggcaacg
      601 atggcttaat tcatcgacac ttagagcttg ttctaagaca gcaatattac cgacttgttt
      661 aggattatca ggatataacg cttcggccat atttaaaccc aacttttggg ctaactgtgc
      721 attattacct aactcagcat gtgcatctaa aggcaaatga taagccaaca acgaaatatc
      781 atgttttaac aacgcttgta ggcgattttt cttcatcccg accacacaag ggttttcact
      841 tttccaaaag aagccatgat gaactaagat tgcatctgct tgtaagtcaa tggctttatc
      901 aattaacgct tggcaagccg tcacacctgt tactaagcgt ttaacctctg cccgcccctc
      961 aacctgcaaa ccattaggac aatagtcact aaaaagatgt ggttttagag ttgtttgtaa
     1021 caattgcata atctctgtta aagaagtcat atttttctct tgatgtattt tacaatgcct
     1081 ccaattatag gctttattgt catttcgcat aggatatgat tatgtctaca ccctattttc
     1141 gtctcatcgc actgagtagt ttaattgccc tcaccgcttg tcaacctaaa gacaaagccg
     1201 aatcttcaac gcccacccaa gttacattaa gtcaggagcg ccctttaacc attcaaggta
     1261 gcgctagtgg tgtgtcatcc tatgcgccaa cagtcaaagc gactgcacct gcggtagtga
     1321 atatttttac gactgcaatt gttaaacata acagccatcc attaatgaat gatccgatgt
     1381 ttcgacgctt ttttgagttt catggcatga atcaaaatga atctgaaccc cgttcaggtt
     1441 taggctctgg tgtgattgtt tccaaagatg gctatatttt aaccaatcat catgtggttg
     1501 ctaaagctga tgaaattatt gtggcgttac atgatggccg caaagccaaa gccaaagtca
     1561 ttggcacaga ccccgacagc gatttggcgg tgattaaaat tgatttaaaa gactt
//
LOCUS       DAOQMB010000529         1602 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_59108_length_1602_cov_24.630252, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000529 DAOQMB010000000
VERSION     DAOQMB010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59108_length_1602_cov_24.630252"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLY05_11775"
     CDS             <1..1335
                     /locus_tag="PLY05_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HNC04018.1"
                     /translation="VLAALSLSTQGLVNVSRDNSLIGAVSLFLMVIAESGERKSACDN
                     LVLKAIKKLNDERFCHDKEQNNDFLTELNIWNSQNNGLHASLTHASKNGEPTDDLKEK
                     IKTHAITKPMQPKGTTFLYEDATIEALTKGLYFNSPSGGIFSSEAGVVFGGHGMNKDN
                     STRNMAALNKLWDDDSIIVGRSDVSKNMLLTGRRLTLCLATQESTVRAFFDGTNGLAR
                     GTGFGARFLIAWPKSTQGTRLYKAPPSHWPHLTRFSSHVLSLLNTDLTIDENTGALQP
                     ITLHLSKQAHAAWVRFYNDTEQELRIGGELTDIKDVTSKAADNVVRIAALFHVYEHGI
                     TGTISEAHIVNAGHIMTWHLMESKRFFNELSLSKELRHATMLDNWLFNYCKEHQTTEI
                     STRTLKQLCPNSLRNKASFDDAISHLIDLNRVKIVNQGKKKLIHLNPKLFEV"
     gene            1337..>1602
                     /locus_tag="PLY05_11780"
     CDS             1337..>1602
                     /locus_tag="PLY05_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04019.1"
                     /translation="MGFADWKGKKQQAATATVATLATVEPKKALKNDNPTLCVADVAS
                     VAVAVSENKQKSEPPTKTELLPNSPVFGGGYSVILANTVKCNQC"
BASE COUNT          533 a          319 c          331 g          419 t
ORIGIN      
        1 gttttagccg cgttatcgct cagtacacaa ggcttagtta atgtatcaag ggataatagc
       61 ctaattggtg cagtctcttt gtttttaatg gtgattgctg aatcaggaga gcgaaaaagt
      121 gcttgtgata atttagtgct taaagccata aaaaaattaa atgatgagcg tttttgccat
      181 gacaaagagc aaaacaatga ttttttaacc gagcttaata tctggaatag tcaaaataat
      241 ggtttacacg catcactaac ccacgcatca aaaaacggtg aaccaacgga cgacctaaaa
      301 gaaaaaatca aaacacacgc aataacaaag cctatgcagc caaagggaac gaccttttta
      361 tatgaagatg caaccataga agcattaaca aagggattat attttaactc ccctagtggc
      421 ggcatttttt caagtgaagc aggtgtcgtg ttcggtggtc atggcatgaa taaagacaat
      481 tccacgcgca acatggccgc actcaataag ctatgggatg atgacagtat cattgttggc
      541 cgatcagatg taagtaaaaa tatgctactt actggcagac gtttgacctt atgcttggcc
      601 acacaggaaa gcaccgtaag agcatttttt gatggtacta atggcttagc aaggggtact
      661 ggctttggtg caagattcct gattgcttgg cctaagtcaa cgcaaggcac acgcctatat
      721 aaagcaccgc ctagccactg gccgcactta acacgcttta gtagccatgt tcttagctta
      781 ttaaatactg acctaaccat tgatgaaaac acaggcgcat tacagcccat cactttgcat
      841 ttatcaaaac aagcacacgc ggcatgggta cgtttttata acgataccga gcaagagcta
      901 cgcataggcg gtgaattaac cgatattaaa gacgtaacga gcaaggcagc cgataacgtg
      961 gtaagaatag ccgccttgtt tcatgtgtac gaacacggca ttactggcac aatctcagag
     1021 gcgcatattg ttaatgcagg tcatattatg acatggcact taatggagtc taaacgcttt
     1081 tttaatgagc tatcattatc caaagagtta cgccatgcaa ccatgcttga taactggctt
     1141 tttaattatt gcaaggaaca tcaaacaacc gaaattagca cccgaacact taaacagtta
     1201 tgccctaatt cattaagaaa caaggcaagt tttgatgatg ctattagcca tttaatagac
     1261 ttaaaccgcg tcaaaatagt caatcaaggc aaaaagaaac taatacacct taatcctaaa
     1321 ttgtttgagg tgtaacatgg gttttgctga ttggaaaggt aaaaaacagc aagctgctac
     1381 tgctacagtc gctacacttg ctacagttga acctaaaaag gcgttaaaaa atgataatcc
     1441 gactctatgt gtagcagatg tagcaagtgt agcagtagca gttagtgaaa ataagcaaaa
     1501 atcagagccg cctaccaaaa cagagttatt accaaatagc cctgtttttg gtggcggtta
     1561 ttctgtcatt ttggccaata ctgttaagtg caaccagtgc ga
//
LOCUS       DAOQMB010000530         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_59256_length_1600_cov_15.295793, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000530 DAOQMB010000000
VERSION     DAOQMB010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59256_length_1600_cov_15.295793"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..491
                     /locus_tag="PLY05_11785"
     CDS             96..491
                     /locus_tag="PLY05_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04020.1"
                     /translation="MEHQHITEQDLHQFFQTFCQDFASFSGEKIAQRYATPYISVNAN
                     GVLSAFLTINEVAALFQGYLDQYREQNCQSCRYDHLAFMLLGANRVVVSVTWQLYNPQ
                     QQLVSTWRESYNLLKNSQGLFIYASTDHA"
     gene            537..995
                     /locus_tag="PLY05_11790"
     CDS             537..995
                     /locus_tag="PLY05_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNC04021.1"
                     /translation="MKNIVKVQKEDFVLDAEIKKLYISNVGAVVSFVGLVREQASHGQ
                     VTAIELEYYPAMTQKALDNIVEQAEQRWPLLGVTVIHRVGYLTAQEQIVMVAVASKHR
                     HAAFAAAEFIMDYLKTQAPFWKKEYIAGQSEGVWVEAKESDNQALAKWQN"
     gene            complement(992..>1600)
                     /gene="fabR"
                     /locus_tag="PLY05_11795"
     CDS             complement(992..>1600)
                     /gene="fabR"
                     /locus_tag="PLY05_11795"
                     /inference="COORDINATES: protein motif:HMM:NF008402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HNC04022.1"
                     /translation="KAVISREDLISAALKLVGPHRSISSLSLREVARAADIAPNSFYR
                     HFKDMDELAIALIDKAGRSLRKIIGEARQRASSERSVIRTSAEIFIEQLNADEALLHI
                     LLREGTVGSPAFKEAVERELTFFEEELCVDLVRLGNAKNLPLYAPELVAKGMTRLVFA
                     MGTTAMDLPRERHSEIIESTVVMLRMILIGAQQMAHNKPDLR"
BASE COUNT          487 a          309 c          333 g          471 t
ORIGIN      
        1 ttggtggcga ttaatcaaga aatggcaact cttgagagtg tggtaaatga aggtgacgaa
       61 gtggcatttt ttccaccagt gacaggcggt taaatatgga acatcagcat attactgagc
      121 aggatttaca tcaatttttt cagacttttt gtcaggattt tgccagtttt agtggtgaga
      181 agattgccca gagatatgca actccctata ttagcgttaa cgccaatggg gtgcttagcg
      241 cgttcttgac cattaatgaa gttgctgcat tgtttcaggg ctatcttgat caatatcgtg
      301 agcaaaattg ccaatcatgt cgttatgatc atttggcttt tatgctcttg ggtgctaacc
      361 gtgttgtcgt gtcagtgaca tggcagcttt ataatccaca acaacaattg gtaagcacgt
      421 ggcgagaatc ctataatttg ctgaaaaatt cacaaggatt atttatttat gcctctactg
      481 accatgccta aacaatcttt attttataca ctccctattt tatagattaa atagatatga
      541 aaaatatcgt caaagttcaa aaagaagatt tcgtgttaga tgccgaaatt aaaaagttat
      601 atataagtaa tgttggtgca gtggtgagtt ttgttggatt ggttcgtgag caagccagtc
      661 atggtcaggt aacagccatt gagcttgaat attatccagc tatgactcaa aaagctttag
      721 acaatattgt tgagcaagca gaacaacgtt ggccattgtt aggtgttacg gtcattcatc
      781 gagtagggta tttaaccgca caagagcaaa ttgtcatggt ggcagtggct agcaagcatc
      841 gccatgccgc atttgctgcg gctgaattta taatggatta tttaaagacc caagcaccct
      901 tttggaaaaa ggaatatatt gcagggcaat cagaaggtgt ttgggtagaa gccaaagaga
      961 gtgataatca ggctttggct aaatggcaaa actaacgtaa atctggctta ttatgagcca
     1021 tttgttgtgc gccaatcaaa atcattctga gcataaccac cgttgactca ataatttcgc
     1081 tatgtcgctc acgtggtaaa tccatagccg ttgtccccat tgcaaacact aaacgggtca
     1141 taccttttgc cactaattca ggggcatata aaggcaaatt tttggcatta cccaatcgga
     1201 ctaaatcaac acacagttct tcctcaaaaa aagtcagctc acgttcaacc gcttctttaa
     1261 aggcaggaga gccaactgtg ccttcacgta ataaaatatg taataacgct tcatccgcat
     1321 ttaattgctc aataaatatt tctgcagatg tacgaatgac actgcgttca ctactcgcac
     1381 gttgtctagc ttcgccaata attttacgca atgagcgtcc tgctttatca atcaaagcaa
     1441 tggccaattc atccatgtct ttaaagtggc gataaaaact attaggcgca atgtcagcag
     1501 cacgagccac ttcacgtagg cttaatgaag aaatactacg atgagggcca actaatttaa
     1561 gggcagcact aattaaatct tcacgagaga taaccgcttt
//
LOCUS       DAOQMB010000531         1596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_59533_length_1596_cov_8.663855, whole genome shotgun sequence.
ACCESSION   DAOQMB010000531 DAOQMB010000000
VERSION     DAOQMB010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59533_length_1596_cov_8.663855"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            277..1305
                     /locus_tag="PLY05_11800"
     CDS             277..1305
                     /locus_tag="PLY05_11800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC04023.1"
                     /translation="MATFLRVLNICVLSLLPMVVYANPPTKKLPPTVEVQAVGQQTLA
                     QQLSALAVLKAQESIMLKPEVAGRVTAINFNEGQVVKEKQLLIQLDDDLLKAELATYQ
                     ASLDLAQTEYQRYQALVKEQQVSVLDYERKKAELAQIKASMALVQAKIRQKQIRAPFT
                     GVIGLRQFSIGDVLQANQNLVRLNKMNALKAEIKIPETHSQSVFVKQMVNLQVDGLAN
                     LVLKGQVAAIEPSLDANTRALIVRVNIPQVDSRVRDGMTARASFVLGQQQAIIVPEQA
                     LVAQGGKFVVFVFNNNEVKATPVEVGQRQVGQVVINKGLQVGDRVVVSGQNKLSKPQM
                     PVKAIPVQ"
     gene            1370..>1596
                     /locus_tag="PLY05_11805"
     CDS             1370..>1596
                     /locus_tag="PLY05_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC04024.1"
                     /translation="MQLPALCIRRPVLAIVLNLLIVLAGLLAFDRLTVREYPNIDIPT
                     VTVDTNYTGASAPIVEATVTKILEDSLSGIEG"
BASE COUNT          501 a          295 c          328 g          472 t
ORIGIN      
        1 gtcggctttt ttattgtcaa aataaaataa catttttatc acttaatgct tctctatgta
       61 aatttttgta ctaataacaa ctttcaacaa aaaaaatcat cttttcacag tacaatcatt
      121 tctaattaag aatgattttc atttttctga tatcttgtca actttaattc agtgaaaacg
      181 tttatcacat gtgaataaag gaaatcgtct acttttgtgc taaggacttt tataaattct
      241 gggctgtgat agcagctgac acctgtggag caaaaattgg ctaccttctt acgtgtatta
      301 aatatttgtg ttcttagttt actgccaatg gtagtttacg ccaatccacc taccaaaaaa
      361 ctgcctccca ccgttgaagt acaagccgta ggccaacaaa ccttggcgca acaactcagt
      421 gctttagcgg tgttaaaagc acaagagtca attatgctta aacccgaagt ggcgggcaga
      481 gtgacggcaa ttaattttaa tgaaggccaa gtggttaaag aaaaacaatt attaattcaa
      541 ttagatgatg atttattaaa agcggaatta gccacctatc aagccagttt agatttagct
      601 caaacggaat atcaacgtta tcaagcatta gttaaagaac aacaagtgtc cgtcttggat
      661 tatgagcgca aaaaagctga attagcgcaa attaaagcga gcatggcttt ggtgcaagct
      721 aaaattcggc aaaaacaaat tcgtgcgcca tttacaggcg tgataggttt gcgccaattt
      781 agtattggtg atgtattaca agctaatcaa aatttagtgc gtttaaataa aatgaatgca
      841 ctcaaagccg aaattaaaat tcccgaaacc catagccagt cagtttttgt caaacaaatg
      901 gtaaatttac aagttgatgg tttggccaat ttagtcttaa aaggccaagt cgctgccata
      961 gagccaagtt tggatgctaa tactcgtgcc ttgattgtta gggttaatat tccacaggta
     1021 gatagtcgag tgcgtgatgg catgaccgcg cgtgccagtt ttgtgttagg tcaacaacaa
     1081 gcgattattg ttcctgagca agccctagta gcgcaaggcg gtaaatttgt tgtctttgtg
     1141 tttaataata atgaggttaa ggccacacca gtcgaagtag ggcaacgcca agtgggtcaa
     1201 gtggtgatta ataaaggttt gcaagtcggt gatagggtag tggtgtcggg acaaaataaa
     1261 ttgtctaaac cacaaatgcc tgtaaaagcg atacctgtgc aatgaccctc accccaaccc
     1321 tctcccaaag ggcgaggcag atgcgtaaat cctgaaaaga gtatcaatta tgcaacttcc
     1381 tgctttatgt attcgccgtc ctgtattagc aattgtactt aacttgttga ttgtattggc
     1441 aggactatta gcttttgacc gccttacggt gcgtgaatat cccaatattg atattccgac
     1501 tgtgactgtt gataccaact acacaggcgc aagtgcgccc attgttgaag caacggtaac
     1561 taaaatttta gaagattcgt tatcgggtat tgaagg
//
LOCUS       DAOQMB010000532         1593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_59745_length_1593_cov_16.267880, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000532 DAOQMB010000000
VERSION     DAOQMB010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1593
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59745_length_1593_cov_16.267880"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>1593
                     /gene="gcvPB"
                     /locus_tag="PLY05_11810"
     CDS             176..>1593
                     /gene="gcvPB"
                     /locus_tag="PLY05_11810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HNC04025.1"
                     /translation="MTLIFERSQEGRRATSQAPLVRHDASDIPAQFRRKKSAVLPEVS
                     ELQVVRHYTNLSRKNFSIDTQFYPLGSCTMKYNPRGAHKGAMMSGFLGRHPLAPVTHS
                     QGFLACMYDLQEVLKEVTGMKGVSLTPAAGAQGEFAGVAMIRAYHQARNDHERSEMLI
                     PAAAHGTNPATAVMCGYKVREIPVLANGDVDVEALKLAVGPQTAGLMMTNPSTCGVFE
                     QQIDQIAQIVHEAGGLLYYDGANLNAILGKVRPGDMGFDVLHMNLHKTFATPHGGGGP
                     GAGPVGVGERLLPFLPTPIVGKEADGRYRWLDKNDLPQSIGHLSAFMGNAGVLLRAYF
                     YAAVLGREGLHRVGEYSTLNANYLMHKLAQAGLQLAYPERRASHEFIITFAKEAKTLG
                     VSAMDIAKRLLDYGFHAPTTYFPLLVPECFLIEPTETEAKDELDNFATALVKILEEAK
                     TEPELVKGAPHTMPVRRLDDVRA"
BASE COUNT          413 a          345 c          382 g          453 t
ORIGIN      
        1 cctgttttag ccaaaatggc cgaattaggc gtgttgggtg gtttggattt aagtccttat
       61 tatcccgaat taggcaatgc cttattggtt tgtaccactg aaaccaaaaa tgccgctgat
      121 ttagcgcgtt ttgctcaagc attaacacaa gctttagctg cttaggaaaa tgaccatgac
      181 cttaattttt gaacgttcac aagaaggccg tcgtgctact tcacaagcac ctttagttcg
      241 tcatgacgcg agtgacattc ctgcacaatt ccgccgcaaa aaatcggctg tgttgccaga
      301 agtatctgag ctacaagttg tccgtcatta caccaattta tcgcgcaaaa actttagtat
      361 tgatactcag ttttatccgc ttggctcttg taccatgaaa tacaacccac gtggtgcgca
      421 taaaggtgca atgatgtctg gctttttagg tcgtcatcct ttagcacccg tgacgcattc
      481 tcaaggcttt ttggcttgta tgtatgactt gcaagaagtt ttgaaagaag tgacaggcat
      541 gaaaggtgtg tccttaacac cagccgcagg tgcacaaggt gagtttgcag gcgtggcgat
      601 gattcgtgcc tatcaccaag cacgtaacga ccatgagcgt agcgaaatgt taattcctgc
      661 tgctgcacat ggtacaaacc cagcaaccgc cgtgatgtgt ggttataagg tgcgtgaaat
      721 tcccgtactt gccaatggtg acgtggacgt tgaagcctta aaactagcgg ttggcccaca
      781 aacagcaggt ttgatgatga ctaacccatc aacctgtggt gtgtttgaac aacaaatcga
      841 ccaaattgcc caaatcgttc acgaagcagg cggcttgtta tattacgatg gtgcaaactt
      901 aaatgcgatt ttaggtaaag tcagacccgg tgatatgggc tttgacgtgt tacacatgaa
      961 cttacacaaa acctttgcta ccccacacgg tggcggtgga ccgggtgctg gccctgtggg
     1021 tgtgggtgag cgtttattac catttttgcc aactccgatt gttggtaaag aagcagatgg
     1081 ccgttatcgt tggttagaca aaaatgactt gccacaaagt attggtcatt tatctgcgtt
     1141 tatgggtaat gctggcgtat tgttgcgtgc ttatttttat gcggctgtgt tgggtcgtga
     1201 aggtttgcat cgtgtgggtg aatactcaac gctcaatgcc aactacttaa tgcacaaatt
     1261 agcacaagct ggcttgcaat tagcctatcc agagcgtcgt gcttcgcatg agtttattat
     1321 tacctttgct aaagaagcga agactttggg tgtatcggca atggatattg ctaagcgttt
     1381 gttggactat ggtttccatg ccccaacaac atacttcccc ttgttagtgc ctgagtgctt
     1441 cttaattgaa ccaactgaga cagaagccaa agatgaatta gacaactttg cgaccgcttt
     1501 agtcaaaatt ttggaagagg ccaaaaccga accagaattg gtcaaaggcg caccgcatac
     1561 catgcctgtt cgtcgtttag atgacgtacg tgc
//
LOCUS       DAOQMB010000533         1590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_59968_length_1590_cov_4.925081, whole genome shotgun sequence.
ACCESSION   DAOQMB010000533 DAOQMB010000000
VERSION     DAOQMB010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59968_length_1590_cov_4.925081"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..1256
                     /locus_tag="PLY05_11815"
     CDS             240..1256
                     /locus_tag="PLY05_11815"
                     /inference="COORDINATES: protein motif:HMM:NF033591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HNC04026.1"
                     /translation="MSLLRLKQVNLTQLALAFSSEASLQSRYRRLQRFFAEVVFNYDH
                     IAHLIMSLFDFEANGYYLALDRTNWQWGKTNINLLTLAVVYQGVAIPVYWLVLNKQGN
                     SNQRERICLINRFIKQFGTNNIKGILGDREFIGGEWWSWLTEHEIPFFIRVKQNQCLN
                     VPHHQPLSDFFRDLTVGQLRCLRKPRLVGQTSVYLSALRLETGELLIIASNMTCANLM
                     DIYALRWQIEHLFQCLKGRGFHFEDMHLTHYFRIKKMMALLAIALAWAHKVGEWQHKR
                     VKPLVIKKHGRLEKSFFRYGLDYLTESLLHYQTDSLRHLRLLLLLFLTPNEISPSNKK
                     RLLS"
     gene            complement(1259..1423)
                     /locus_tag="PLY05_11820"
     CDS             complement(1259..1423)
                     /locus_tag="PLY05_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04027.1"
                     /translation="MKNKLFIASLGFILLVGCNTAPSKSYTPKDGNSNWAITGRYNGI
                     TRNLCPRQFL"
BASE COUNT          498 a          307 c          319 g          466 t
ORIGIN      
        1 cattaaggat catagggata tttgtgagcg tgccagtaaa agctggcaag gcgatctctg
       61 tacacgacaa aaaagcattt tgagttgaaa aagagttagg tagccgccat tcaggccatc
      121 acgatttaac gaccaagaaa aaaagtgatg ctatgaacct gaatgacgga ctagtaggca
      181 gtctaaagca ctatttaccc ttgaacaaga gtcgcctaag ctgtttatgt gggatattat
      241 tgagcttatt acgcctaaaa caagtgaatc taacgcaatt ggctttagcc tttagcagtg
      301 aagcctccct ccaatcacgt taccgccgct tacaacgatt tttcgctgaa gtcgtattta
      361 actatgacca tatcgcccac ctcatcatga gtttgttcga ttttgaagcg aatggctact
      421 accttgcttt agaccgtacg aattggcaat ggggaaaaac aaacattaac ctcttaacct
      481 tggccgtggt atatcaaggt gtggcgattc ctgtgtattg gctagtgttg aataaacaag
      541 gtaactccaa tcagcgagag cgcatctgct taatcaatcg gtttatcaaa caatttggga
      601 caaataatat caaggggatt ttaggagata gagaatttat cgggggtgaa tggtggtcat
      661 ggctgacaga acacgagatt ccttttttta ttcgggttaa acaaaatcaa tgccttaatg
      721 tgccacatca tcaaccattg agtgattttt ttcgtgattt aaccgtagga cagttacgct
      781 gtttacgcaa acctcgttta gtgggtcaaa cgtctgttta tttgagtgct ttgcgcttag
      841 agactgggga attgctgatt attgcaagta atatgacctg tgctaatctc atggatattt
      901 atgccttacg ttggcagatt gaacatttat ttcagtgtct taaagggcgc ggctttcatt
      961 ttgaggatat gcacttaacc cattattttc gcatcaaaaa aatgatggca cttttagcca
     1021 ttgcattagc gtgggcgcat aaagtcggtg aatggcagca caagagagtg aaacctttag
     1081 tgattaaaaa acatggtcgt ctagagaaaa gcttctttcg atatggcttg gattatttaa
     1141 cagagtcctt gttgcactat caaacagact ctttgcgtca cttacgctta ttgctgttgc
     1201 tattcttaac ccctaatgaa attagtcctt caaataaaaa acgcctactt tcataaactt
     1261 acaaaaattg tcgtggacag aggttacgag taataccatt atacctgcca gtaatagccc
     1321 aattactatt accatctttt ggtgtatagc tttttgatgg tgcagtatta caaccaacca
     1381 aaagaataaa acctaaagac gcaataaaaa gcttattttt cataaaccct ccagcctaac
     1441 ttaaaattaa ctggcacaac cccacaacaa aataaacaag tgagagtttg ccaaaaatat
     1501 aaaaaatatc accgtcatcg gtcggggttt tgtccagttg aatgattagt taggataaag
     1561 tgcaatgcac tgaaaactaa actcacaaca
//
LOCUS       DAOQMB010000534         1589 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60033_length_1589_cov_8.387223, whole genome shotgun sequence.
ACCESSION   DAOQMB010000534 DAOQMB010000000
VERSION     DAOQMB010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60033_length_1589_cov_8.387223"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1443)
                     /locus_tag="PLY05_11825"
     CDS             complement(133..1443)
                     /locus_tag="PLY05_11825"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNC04028.1"
                     /translation="MKVLPRNKDTFLQTVLQVLPTDYQEQAYQQGAFTRSRKLTSPAQ
                     LFELVMSYCCLDYSLRTCAGHMSANKTAALSDTAVMKRLLPCLPWLKCLLSSVLLNKL
                     SKTPTNSPYRFLAIDASTVQVKAARSTSYRLHLAMDLITLAQTQIKITDAHQGENLSH
                     YDLKANDVVLVDRGYNQPKTLVPFIERGGYIVLRYNPKGMTLYHPPQENSLLQRIDWV
                     KEVSNVQGNSACKEVMICYDNKLFKGYVHLSRLSKEHAEKAHREVKRSAQRQQYTLSQ
                     NALTLADWMFVFTTLPPDVLETKMVMALYRTRWQIELVFKRLKSLLSLTELRAKQGSR
                     LAEIYLYGKLLYAAMLECYLSQYVNVQAYYLDNETNDRLETPWRWWHLFHQQFMAYFS
                     IYLSPSNIPIQAIQKAMRERKRKRKLQTIPNNLMKNLHKINNLL"
BASE COUNT          461 a          309 c          309 g          510 t
ORIGIN      
        1 aactttaacg ctaagaggta gcacccctca acaaactata acctgaggtt gacctcagaa
       61 ccattcaaaa atacatcaaa accgcgcggg gtgctatccc atatgcgcca acttaagcta
      121 actttttgtt atttataaca aattatttat tttgtgtaag tttttcatta agttgttagg
      181 tattgtctgt agttttctct tccgttttcg ctcacgcatc gccttttgta tcgcctgtat
      241 aggtatgttt gaaggagata agtagatact aaaataagcc ataaactgct gatgaaataa
      301 atgccaccat cgccaagggg tctcaagcct atcattcgtt tcattatcta aataatacgc
      361 ctgcacattc acgtattggc ttaaatagca ctcaagcatc gctgcataaa gtagttttcc
      421 atagagataa atctccgcca atcgactccc ttgtttagca cgtaattcag ttaaagataa
      481 taggctcttt aaacgtttaa agactaactc aatttgccag cgtgtgcgat ataaggccat
      541 caccattttg gtctctaaaa catcaggagg taaggtagtg aaaacaaaca tccaatccgc
      601 taacgtcaag gcattctgag agagcgtata ttgttgtctt tgtgccgagc gtttaacctc
      661 gcgatgggct ttttcggcat gctctttaga taaacgtgaa agatgaacat accctttgaa
      721 tagcttattg tcataacaaa tcatcacttc tttacatgca ctattacctt gtacattact
      781 gacttcttta acccaatcaa tacgttgcaa taaactgttc tcttggggtg gatgataaag
      841 tgtcatgcct ttaggattat aacgtaatac aatataaccg cctctttcaa taaaaggaac
      901 gagtgttttg ggttgattat agcctctgtc aacaagaaca acatcgttgg cttttaaatc
      961 ataatgactt aaattttcac cttgatgagc atcggttatt ttgatttgtg tttgtgcgag
     1021 agtgattaaa tccatcgcta aatgtaagcg ataactggta cttcttgctg ctttgacttg
     1081 aacggttgag gcatcaatgg cgagaaacct atatgggcta tttgttgggg tttttgataa
     1141 cttatttaat aatacgcttg atagtaaaca ctttaaccat ggcaagcatg gcagtaatcg
     1201 tttcatgacc gctgtatctg atagagcagc cgtcttattt gctgacatat gacctgcaca
     1261 ggtgcgtaat gaataatcta aacaacaata gctcatcact aattcaaata attgcgcagg
     1321 tgaggtcagt ttgcgtgagc gtgtaaacgc gccttgttgg taggcttgtt cttggtaatc
     1381 agtgggtaat acttgtaaca ctgtctgtaa aaaggtatct ttgttgcgag gtagtacttt
     1441 catagcagcc tagactgggg agtcaaatct actatgtggg tactatctcg ctgttttcaa
     1501 ataccttttt tccttaagtt ggcgcatatg gggtgttatc ccacttgagc gttaagttag
     1561 acgctaaaac ataaacaaga cactttgac
//
LOCUS       DAOQMB010000535         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60187_length_1587_cov_14.928851, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000535 DAOQMB010000000
VERSION     DAOQMB010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60187_length_1587_cov_14.928851"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="PLY05_11830"
     CDS             <1..843
                     /locus_tag="PLY05_11830"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04029.1"
                     /translation="MKDLKNKVAVITGAAEGIGKAIAQQAAQHGMKLALADINSQKLQ
                     TTVDEFVAQGVEVFSQAFDVANHQQVDAFANAVFAHFGNVHLLVNNAGVAISRPVWET
                     TPEDWQWVMGVNFYGVTNGLRAFIPRMLANNEEGHIVNTASVAGMLSLPCTAAYNASK
                     HAVVTVSEGLFFDLQLRQAKLKVSVLCPAWVKTGIAEAERNRTVGQSIDFSQADQVNR
                     FTAGMVQQAVAKGIPVEKVANDVFTAIQEERFYIFTHAKIKSSIQLRMEDILQERNPT
                     LVSL"
     gene            845..>1587
                     /locus_tag="PLY05_11835"
     CDS             845..>1587
                     /locus_tag="PLY05_11835"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNC04030.1"
                     /translation="MPYLTVNGANIYYEEHGQGEQTIVFAHGLLWSGQMYEAQVAFFK
                     QHYRCITFDFRAQGRSQATESGYDMDTLAADALALIKQLVGKPVHFVGLSMGGFVGMR
                     LAIHHREWLLSLSLLETSADPEPKQNLLKYKVLPKIACWLGMAVVAPQAMHIMFGQTF
                     LTDSRRAEQRAEMRRRLLQNDRINIGKAINGVFSRQGVYQYLPSITTPTLIVVGDEDI
                     ATIPAKSRRMHAAIKHSQLVVIPQAGHTST"
BASE COUNT          430 a          313 c          382 g          462 t
ORIGIN      
        1 atgaaagatt taaaaaacaa agttgcggtg attacgggtg cagccgaagg cattggcaag
       61 gcgattgctc aacaagcggc acaacacggc atgaagctcg ctttagccga tattaatagc
      121 caaaaattac aaacaaccgt cgatgagttt gttgcacaag gggtcgaggt atttagccaa
      181 gcgtttgatg tggctaatca ccaacaggtt gatgcctttg ccaatgcggt atttgcccat
      241 tttggtaacg ttcatctgtt agtcaataat gcaggggtgg cgatttctcg gcctgtttgg
      301 gaaaccacac ctgaagattg gcaatgggtg atgggggtta atttttatgg ggtaaccaat
      361 ggtttaagag cttttattcc ccgaatgtta gccaacaatg aagaagggca tattgttaat
      421 acagcatcag ttgcaggcat gttatcgttg ccatgtactg ccgcttataa tgccagtaaa
      481 catgcggtgg tgacggtttc tgagggtttg ttttttgatt tacaactgcg tcaagctaag
      541 ttaaaagttt cggtattgtg tcctgcatgg gtcaaaacag gcattgccga ggcagagcgt
      601 aatcgcactg ttggccaaag cattgatttt agtcaagccg atcaagttaa tcgttttacc
      661 gcaggtatgg tgcaacaagc agtggcgaaa ggtattcctg tcgaaaaagt ggctaatgat
      721 gtctttacgg cgattcaaga agaacgcttt tacatcttta cccatgccaa aattaaatca
      781 tccatacaac tacgcatgga agatatttta caagagcgta atccaacttt agtgagtttg
      841 tgacatgcct tatttaacgg taaatggtgc aaatatttat tacgaagagc atggccaagg
      901 tgaacaaacc attgtttttg cgcatggttt gttgtggagt ggtcaaatgt atgaggcaca
      961 agttgccttc ttcaaacagc attatcgttg tattaccttt gattttagag cacaaggccg
     1021 ttcccaagcg accgagagtg gttatgacat ggacacactc gctgctgatg ctttggcttt
     1081 gattaaacaa ttggttggca aacctgtgca ttttgttggt ttgtcgatgg gcggttttgt
     1141 cggtatgcgt ttggccattc atcatcgtga atggttgttg tctttaagtt tgctcgaaac
     1201 ttccgccgac ccagaaccta agcaaaattt gttaaaatat aaagtcttac ccaaaattgc
     1261 ctgttggttg gggatggccg tggttgcgcc acaagcgatg cacatcatgt ttggtcaaac
     1321 ctttttaact gactctcgtc gtgctgaaca acgcgccgaa atgcgtcggc gtttgttgca
     1381 aaacgaccga atcaatattg gcaaagccat taatggtgtg tttagtcgac aaggggttta
     1441 tcaatattta ccttcaatta cgacacccac tttgattgtt gtcggtgatg aagatattgc
     1501 caccattcct gccaaatcac gacgcatgca cgccgcaatt aaacacagtc aattggtggt
     1561 gattccacaa gcagggcata cctcaac
//
LOCUS       DAOQMB010000536         1587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60189_length_1587_cov_13.560052, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000536 DAOQMB010000000
VERSION     DAOQMB010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60189_length_1587_cov_13.560052"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLY05_11840"
     CDS             complement(<1..1183)
                     /locus_tag="PLY05_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HNC04031.1"
                     /translation="MNLNIPHKLIPSFENLKTNQYDTIVWYGGRGSAKTNALIKIGIL
                     ESFIDDGVILCCREIQKSIADSLYSAIVSFITDNKLDNIFKITLGEIVNLQTNARFIF
                     AGLKSNITSIKSIDKLRVVLTDEAENITQQSWDYLRPTPRYGHTRTYVVFNPRFEQDP
                     TWQEFVIRADDNTLLTEINWRDNPFFPESLERQRQRSLQGDIGRYNWIWEGKFLQVSD
                     ASILAKKLKVQDFDITEDFSTPYIGVDWGFSVDPTAAIECYVFSDNLYIRRACSKVGL
                     ELDDTADYLIKHIPSIKKYTSRADNARPETISKVKKDISLITACKKWKGSVEDGVVYL
                     QSFKQIIVHPEAECCLAELSAYNYKVDSNGDPTTVIDDASNHYADALRYAIEPLLRKD
                     PE"
     gene            complement(1173..1571)
                     /locus_tag="PLY05_11845"
     CDS             complement(1173..1571)
                     /locus_tag="PLY05_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04032.1"
                     /translation="MANPVGRPRTKLNDLPADWESIICMAAQDGASDVELRCLLGIGE
                     SAWGTLLEDSEEFRGTIKKAHDLCRVWWERHGRKMTTGEADGNATVWIFNMKNRFGWN
                     DKQQLDHTSSDGTMKTPFFNIVGVAPKDES"
BASE COUNT          431 a          356 c          296 g          504 t
ORIGIN      
        1 tttcggggtc tttacggagt aacggctcta tcgcatagcg taaagcatca gcatagtgat
       61 tgcttgcgtc atcaataact gttgtcgggt cgccattgct atctactttg taattataag
      121 ccgataattc ggcaaggcaa cattcagctt caggatgcac aatgatttgc ttaaatgact
      181 gcaaatatac cacaccatct tcaacgctgc ccttccattt tttacacgct gttattaagc
      241 taatgtcttt ttttactttg ctaatagttt cgggtctagc attatcagcg cgtgatgtgt
      301 attttttaat gcttggtatg tgcttaatta gatagtctgc cgtgtcgtct aactcaagcc
      361 cgacttttga gcaagcacgt ctaatgtaca aattatcgct aaacacataa cactcaatag
      421 cggctgttgg gtcaacgcta aacccccaat caacaccgat atacggtgtg ctaaaatctt
      481 cagttatatc aaagtcttgt acttttaatt ttttggccaa gattgacgca tcactgactt
      541 gtaaaaattt gccttcccat atccagttat atcggcctat gtcgccctgc aaactgcgtt
      601 gcctttggcg ttctagcgac tcgggaaaga atggattgtc gcgccagttt atctcagtta
      661 atagcgtatt atcatcggct cttataacaa actcttgcca tgtcgggtct tgctcaaaac
      721 gcggattaaa aacaacataa gtacgggtat gaccatatcg cggagtaggg cgcaaatagt
      781 cccaagattg ttgtgtgatg ttttcagctt catcggttaa aacaacacgc aatttatcaa
      841 tagacttgat gctagtgatg tttgatttta agccagcaaa aataaaacgc gcatttgttt
      901 gcaggtttac tatttcgcca agtgtgattt tgaatatatt gtctagttta ttgtcagtga
      961 taaagctaac gattgcagaa taaaggctat cagcgattga tttttgaatc tcacgacagc
     1021 ataaaatcac gccatcatca ataaatgatt ctaagattcc gattttaatt aaagcattgg
     1081 tttttgcact gccgcgtccc ccataccaca caatcgtatc gtattggtta gtctttagat
     1141 tttcaaatga tggtatgagt ttatgcggta tattaagatt catctttggg ggctacccct
     1201 acgatgttaa aaaatggggt tttcatcgtg ccatcgctcg atgtgtggtc tagctgctgt
     1261 ttatcattcc agccgaatct attcttcata ttaaaaatcc aaaccgtagc atttccatca
     1321 gcttcgcctg tggtcatttt ccgcccgtga cgctcccacc aaacacggca aaggtcatgt
     1381 gcttttttta tggttccacg aaattcttca ctatcttcta aaagtgttcc ccatgccgac
     1441 tccccaatac ctaaaaggca tctcaattca acatcggacg cgccatcctg agcagccata
     1501 catataatgc tttcccaatc agcaggcaag tcatttaatt ttgtacgcgg tctccccaca
     1561 ggattagcca taatcacaac accttaa
//
LOCUS       DAOQMB010000537         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60262_length_1586_cov_13.573481, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000537 DAOQMB010000000
VERSION     DAOQMB010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60262_length_1586_cov_13.573481"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="rfbB"
                     /locus_tag="PLY05_11850"
     CDS             complement(<1..1004)
                     /gene="rfbB"
                     /locus_tag="PLY05_11850"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004867424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNC04033.1"
                     /translation="MTILVTGGAGFIGSNFVLDWLAQSDEPIVNLDKLTYAGNLDSLK
                     SLKDDARHIFVQGDIGDKALVSQLLAQYQPRAILNFAAESHVDRSIKSPTNFIDTNII
                     GTFNLLEAVRAYWLSLDQPLQTSFRLLHVSTDEVYGSLLPQDPAFAETNQYQPNSPYS
                     ASKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLVILNALAGKPLPIYGD
                     GQQIRDWLYVKDHCAAIRCVLANGRVGETYNVGGWNEKANLEVVKTICALLDELQPKT
                     SGSYAEQISFVQDRAGHDRRYAIDARKLEQELGWKPQETFETGLRKTVLWYLNHQDWV
                     "
     gene            complement(1036..>1586)
                     /locus_tag="PLY05_11855"
     CDS             complement(1036..>1586)
                     /locus_tag="PLY05_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04034.1"
                     /translation="CSALEFVDKDLVHLGMSNLNFKPSFGNALVENIVTGCTAVFNNS
                     LKKLVKSYQPQQAVMHDWWLYLLATAFGQVIYDPIAYIKYRQHGANVIGATPSFMHLW
                     RRRLTILLQAKPIVSTLQAAEFYQYYNQYLSKEYLELAKKFVERRSNSNARWFIVYSA
                     LIYKQNSLDTIAMKLLIALGRY"
BASE COUNT          507 a          327 c          275 g          477 t
ORIGIN      
        1 tttacccaat cctgatgatt taaataccac aataccgttt tacgtagtcc tgtttcaaag
       61 gtttcttgtg gcttccaacc taactcttgt tctaatttac gggcatcaat cgcataacgg
      121 cgatcatggc ctgcacggtc ttggacaaaa ctaatttgct ctgcatacga gcctgatgtt
      181 ttgggttgta actcatccaa taacgcacaa atggttttta cgacctctaa attagctttt
      241 tcattccacc caccgacatt ataagtttcc cccactcggc cattagccaa cacacaacga
      301 attgccgcac aatggtcttt aacatataac caatcacgaa tttgttggcc atcgccataa
      361 attggcaacg gtttgcctgc taaagcattt aaaattacta aaggaattaa cttttcagga
      421 aaatgatatg gcccatagtt attgctgcaa ttagtcgtta acacaggcaa gccataggta
      481 tgatgccatg cacgcactaa atgatctgag gcagccttag aggccgaata aggactatta
      541 ggttgatatt gattggtttc ggcaaaagcg ggatcttgag gcaataaaga accatagact
      601 tcatcggtag aaacatgtaa caagcgaaaa cttgtctgaa gcggctgatc taggcttaac
      661 caatacgctc tgactgcttc tagtaagtta aaagtgccaa taatgttggt atctataaaa
      721 ttggtagggc ttttaatcga acgatcaacg tggctttcgg cagcaaaatt taaaatggca
      781 cgcggttgat attgtgctaa taattggcta accaacgctt tatcaccaat atcaccttga
      841 acaaagatat ggcgagcgtc atcttttaag ctttttaatg aatccaaatt gcctgcatag
      901 gttaatttat ctaaattaac aatcggttca tcactttgtg ccaaccaatc caagacaaag
      961 tttgagccaa taaaacctgc accacctgtg actaatattg tcatactgat attctctaca
     1021 caaaataaaa attgattaat atcgccccaa agcaattaag agtttcattg caatggtgtc
     1081 caaactattt tgtttataga ttaatgcact ataaacaata aaccaacgag cattgctatt
     1141 cgatcttctt tcaacaaact tttttgctaa ttcaagatat tcttttgaaa gatattgatt
     1201 gtaatattga taaaactctg ctgcttgcag cgttgaaaca attggcttag cctgtaataa
     1261 aatagtcaat cttctacgcc acaaatgcat aaagcttggt gttgcgccaa taacattagc
     1321 tccatgctga cgatacttaa tatatgcaat tggatcataa ataacttggc caaatgccgt
     1381 ggcaagcaaa tataaccacc aatcgtgcat gaccgcttgt tggggctgat aactctttac
     1441 taattttttg agtgaattat taaatacagc agtacaaccc gtgactatgt tttcaaccaa
     1501 ggcatttcca aaagagggtt tgaagtttaa gtttgacatg cccaaatgta ctaaatcttt
     1561 gtcaacaaac tctaatgccg aacaat
//
LOCUS       DAOQMB010000538         1586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60265_length_1586_cov_9.256695, whole genome shotgun sequence.
ACCESSION   DAOQMB010000538 DAOQMB010000000
VERSION     DAOQMB010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60265_length_1586_cov_9.256695"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(81..473)
                     /locus_tag="PLY05_11860"
     CDS             complement(81..473)
                     /locus_tag="PLY05_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004719756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HNC04035.1"
                     /translation="MTESDDKVRIDKWLWAARFFKTRSLAKDAIEGGKVHCEGQRVKV
                     SKEAKVGMELTIKQGFEDKTVVITALSEVRRGAPEAQLLYSETAESVAKRQHLNEQRK
                     AANLAYPDHRPNKKERRQIHKFQDSWRD"
     gene            512..>1586
                     /locus_tag="PLY05_11865"
     CDS             512..>1586
                     /locus_tag="PLY05_11865"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNC04036.1"
                     /translation="MHDAVPFLRRQPVIILFGVVAWLLAVWQLTQVAVLQGKSLYFLL
                     IALNGIGALFALLRLFWLIVTSRRWQIDNQDLFIWGCLVAGYGSAFWLAHYLILPPIE
                     SFFLCFLLVNFAPLLNRFSALVANSVFLLTSVTVAALQHTLPFVVFMLFLLLAQSALW
                     YMAFCNIAEWQAHQETATAHAQLRATQQLLSEAVARNERTRIARDLHDQMGHHLVALT
                     IQLQILERKLPESVVGELSQVQDLAKELFSDVRTTVQQLRDEQSSFNILLESMLSNIP
                     FLEFDVDVDQDVVELIDEQMANCLLRVVQEAITNSLKHSDACRLWISLLPHENGILLL
                     VKDNGRPRKTVDFSSGNGLRGLEE"
BASE COUNT          434 a          286 c          334 g          532 t
ORIGIN      
        1 aaccaacgca aaaatgcgcc ctgagcggag tcgaagggta ggctatgttc tttaaatgaa
       61 tggctgtagg agagtactta tcagtctcgc catgagtctt gaaacttatg aatctgccgc
      121 cgttcttttt tgttagggcg gtggtcagga taggctaagt ttgcagcttt acgctgttca
      181 tttaaatgct gacgtttagc aacactttcg gctgtttcac tatataacaa ttgagcttcg
      241 ggcgcaccac gacgcacttc agagagagcc gtaatgacca cagttttatc ttcaaagcct
      301 tgtttaatgg ttaattccat accaactttg gcttctttac tgactttaac acgttgccct
      361 tcacagtgaa ctttgccacc ttcaatggca tcttttgcta acgaacgtgt tttaaaaaaa
      421 cgtgcagccc atagccattt atcaattcgc actttatcat cggactcagt cattaaatat
      481 tctctaaaaa aatttgcaga agaataatat tatgcatgat gcagtgccat ttttacgtcg
      541 tcaacctgtg attattttat ttggggtagt agcgtggttg ctagctgttt ggcaactaac
      601 tcaagttgct gttttgcaag gtaaaagtct ttatttttta ctcattgcac ttaatggtat
      661 aggtgcatta tttgcgctat taaggctttt ttggctgata gtaacctcaa gacgttggca
      721 aatagataat caagatttgt ttatttgggg ttgcttggtg gcaggctatg gcagtgcttt
      781 ttggttggca cattatttaa tcttgccccc catcgaaagt ttctttttat gttttttatt
      841 agtcaatttt gcgccattgt taaatcgttt tagtgcctta gtcgccaata gtgttttttt
      901 attgacatct gtcactgttg ccgccttaca acatacctta ccctttgtcg tgtttatgct
      961 gtttttgtta ttagcacaaa gtgctttatg gtatatggct ttttgtaata ttgctgaatg
     1021 gcaagcccat caagaaacag caacggcgca tgctcaactt agagcaacac aacaattatt
     1081 aagtgaagcc gtggcaagaa atgaacgaac acggattgcc cgtgatttgc acgaccaaat
     1141 gggacatcat ttagtcgcat tgaccataca attacaaatt ttagaacgca aattgccaga
     1201 atctgtggtt ggtgagttaa gtcaagttca agatttggcc aaagaattat ttagcgatgt
     1261 acgcacgaca gttcagcaat tacgtgacga acaatcgagt tttaatatat tgctagagtc
     1321 tatgttgtca aatattccat ttttggaatt tgatgttgat gttgatcaag acgttgttga
     1381 gttgatagat gaacaaatgg ctaattgctt gttaagggta gtacaagaag cgattaccaa
     1441 tagtttaaaa cacagcgatg cttgtcggtt gtggattagt ttgctgccac acgaaaatgg
     1501 cattttatta ttagtcaaag ataatggtcg gccacgtaaa acagttgatt ttagttcagg
     1561 aaatggctta cgcggtttag aagaaa
//
LOCUS       DAOQMB010000539         1583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_60513_length_1583_cov_6.952225, whole genome shotgun sequence.
ACCESSION   DAOQMB010000539 DAOQMB010000000
VERSION     DAOQMB010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60513_length_1583_cov_6.952225"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1583
                     /gene="pglZ"
                     /locus_tag="PLY05_11870"
     CDS             16..>1583
                     /gene="pglZ"
                     /locus_tag="PLY05_11870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HNC04037.1"
                     /translation="MSNSKIQQALQNLFEKHRIVFWYDAKQEFVNDFEQLTLANIEKI
                     QLNNNEFGVKYRILRQEPEDKFLLYAAYAQPEDLENWLLDIQLAHGEFRTDQVALWLA
                     ELELGLGFADVIKAHSEFFQASKRKDSLKRVLVADDTPHLVRLKMLAICSSADSGLDT
                     ILESLLAELADDRDDKIRLIQRCSLDGFLWQQLQRHYGYESTSPSIKDFSLELFKSCY
                     ALATAQTAKLSHHALVFLKRWKDSVKHRQYFEKLSENCAHDLGLEQLLETQDFRQLMD
                     IDYVELIDRKILCELVQGVTQRTLLHNEVSQWVRQRRSSYWYDRFAHVYNAIDAASAF
                     IFTLNSTKLNPLSIEDGIRRYSQEWFKLDQYYRKFIYHLRASKRPSLLNALYTLIDNL
                     YSNNYLLTLNDRWQVLVDNAPTWESSPFPSQRHFFNRSVKPFLERNAKVCVIISDALR
                     YEIGEELLSLVRQEDRYDANLEPMVSMLPSYTQLGMAALLPHQQLAIADNQGETLVDG
                     QSSKGSDNRKKILN"
BASE COUNT          471 a          358 c          318 g          436 t
ORIGIN      
        1 aattaggagc aacgggtgag taacagtaaa atccaacagg cactacaaaa tctatttgaa
       61 aagcatcgta tcgttttttg gtacgatgcc aaacaagaat ttgtgaatga ctttgaacaa
      121 cttaccttag caaatataga aaaaattcag cttaataata atgagtttgg tgttaagtat
      181 cggattttac gccaagagcc agaagataaa tttttactat atgccgccta tgctcaaccc
      241 gaagatttag aaaattggct attagatata caactggcac atggcgaatt tagaaccgac
      301 caagtggctt tatggttagc cgaattagaa ctgggtttag gttttgccga tgttatcaaa
      361 gcccatagcg aattttttca agccagcaaa cgtaaagaca gcctcaaacg agtattagtt
      421 gcggacgaca cacctcacct tgtccgccta aaaatgttgg ccatttgcag ctctgccgac
      481 agtggccttg ataccatttt agaaagccta ctggcggagt tggctgacga ccgtgatgac
      541 aaaattcgtc ttattcagcg ttgcagccta gacggttttt tatggcaaca actgcaacgg
      601 cattatggtt atgaatctac tagccccagc atcaaagact ttagcctaga gctgtttaag
      661 tcgtgttatg cccttgcaac cgcacaaaca gccaaactca gccatcatgc ccttgttttt
      721 ttaaagcgtt ggaaagatag cgtcaaacat cgtcagtatt ttgaaaaact ctccgagaac
      781 tgcgcccatg accttggcct tgagcaatta ttagaaacac aagactttcg tcagctcatg
      841 gacatagatt atgtcgaact cattgaccgt aaaatcttgt gtgaattagt gcaaggtgta
      901 acccagcgca ctctgcttca taacgaagtc agtcaatggg tacgtcaacg ccgtagcagt
      961 tattggtacg accgctttgc ccatgtctat aacgccatag atgcagcatc agcgtttatt
     1021 tttacgctca atagtaccaa actcaatcct ctttctattg aagatggtat tagacgttat
     1081 agccaagaat ggtttaagct cgaccaatac taccgtaaat ttatttacca tctgcgcgca
     1141 tcgaagcgac cctcactact caatgcactt tataccctga tagacaacct atacagcaat
     1201 aattacttgc tcacccttaa cgatcgttgg caagtattgg tggacaacgc tccaacatgg
     1261 gagagttcgc ccttcccctc tcagcgtcat ttttttaatc gctcggtcaa accgttttta
     1321 gaacgtaatg ccaaagtctg cgttattatc tcggacgcat tacgctatga aataggcgaa
     1381 gagctattgt cgttggtgcg ccaagaagac cgctatgatg ccaatttaga gccaatggtg
     1441 tctatgttgc ctagctatac tcaattaggt atggccgccc tactccctca tcaacagctt
     1501 gccattgctg ataatcaagg cgaaaccctt gttgatggcc aaagtagcaa aggcagtgat
     1561 aaccgtaaaa agatactcaa taa
//
LOCUS       DAOQMB010000540         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_61107_length_1575_cov_16.810526, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000540 DAOQMB010000000
VERSION     DAOQMB010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61107_length_1575_cov_16.810526"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1168)
                     /locus_tag="PLY05_11875"
     CDS             complement(98..1168)
                     /locus_tag="PLY05_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HNC04038.1"
                     /translation="MKHFRFSFIFTFICLGLSAWWGFTHGPEAGLNTMFKALLITAIL
                     AVMEVSLSFDNAVVNASVLRNWDEFWKTIFLTVGILIAVFGMRLVFPLLIVGVTADMS
                     MVEVAKLALNDPKSYSEKLMAHHAEIAAFGGLFLLLVFLNFLFDDEKDTHWFHWLEHK
                     LANLANVPAMSVFIALIALLIMVSAVEGETKLVVTIAGIWGIVVYVGVKALGHLLEST
                     SGNDDEEKASSDVSGNVVKAGIGGFLYLEVLDASFSFDGVIGAFAITNDVVIIMLGLA
                     IGAMFVRSMTIFLVEKGTLEAYIYLEHGAHYAIGALAVIMLFSGTGVHVPEVLTGLIG
                     VAFIVWAVISSIQYKKSEAGQA"
     gene            complement(1240..>1575)
                     /locus_tag="PLY05_11880"
     CDS             complement(1240..>1575)
                     /locus_tag="PLY05_11880"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017735686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HNC04039.1"
                     /translation="SVPQDWLAIINEFGGDIKETYGVPVEQIVEAIKHGVRKVNIDTD
                     LRLASTGAIRQFMAKNPAEFDPRKYLAKTVTAMRDICIDRYEAFGAAGNASKINPISL
                     EKMVSRYSK"
BASE COUNT          556 a          376 c          234 g          409 t
ORIGIN      
        1 ccaaacgtta cagtcaaccc ttacaaaata ttattgaatt taaaaaagcc agttaataaa
       61 aaagcgcgat accgcgcttt tttatttttt gcgatgatta agcttgtcct gcttccgatt
      121 ttttatattg aatggaggaa atcaccgccc aaacaataaa ggccacccca atcaatccag
      181 tcaatacctc tggcacatgc acgcctgtac cactaaagag cataatcact gctaaagcac
      241 caatcgcata atgtgcgcca tgctctaaat aaatgtaggc ttccaatgtc cctttttcga
      301 ctaaaaatat cgtcattgaa cgcacaaaca ttgcaccaat agccaaacct aacataataa
      361 tgacaacatc attggtaatc gcaaacgcac caattacacc gtcaaaacta aatgaagcat
      421 ctaaaacttc taaatataaa aagccgccaa tacctgcttt aacaacattc ccagaaacat
      481 cagaagaggc tttttcttcg tcatcattac cgcttgtgct ttctaagaga tggcctaagg
      541 ctttaacccc cacataaacc acaatccccc agatacctgc tatagtgact accaatttag
      601 tttcaccttc aacagcactg accatgatta ataaagcaat taaagcaata aacaccgaca
      661 tcgcaggtac attggctaaa ttagctaatt tatgctctag ccaatgaaac caatgggtat
      721 ctttttcatc atcaaataag aagtttaaaa ataccagtaa taaaaataaa ccaccaaacg
      781 cggcaatttc agcatgatgc gccatcaatt tttcagaata cgacttaggg tcattaaggg
      841 ctaatttagc cacttcaacc atactcatat cagccgttac cccaacaatt aatagaggga
      901 aaactaagcg cataccaaaa acagcaatta aaatacccac cgttaagaaa atagttttcc
      961 aaaactcatc ccaattacgt aacacagagg cattcaccac cgcattatca aacgataaag
     1021 atacttccat caccgccaaa atagccgtaa ttaataatgc cttaaacatg gtattaaggc
     1081 ctgcttctgg accatgagta aacccccacc atgccgaaag gccaaggcaa ataaaggtaa
     1141 aaataaacga aaaacgaaaa tgtttcacaa tcaatatcct agaatgagac taacaacaaa
     1201 aaggtcagca attgctgacc tttttgtcac cagtcgtgat tatttactat aacgactgac
     1261 catcttttct aaagagatgg ggttaatttt gctggcatta cctgctgcac caaaagcttc
     1321 ataacggtcg atacaaatat cacgcattgc cgtcaccgtt ttggctaaat acttacgtgg
     1381 gtcgaattcg gctggatttt tcgccataaa ttgacgaatt gcacctgtag aggccaaacg
     1441 taaatcggtg tcaatattca ctttacgcac gccgtgttta atcgcttcta cgatttgttc
     1501 aacaggcacg ccataagttt ctttaatatc accaccaaat tcattaataa tggctaacca
     1561 atcttgtggt acaga
//
LOCUS       DAOQMB010000541         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_61427_length_1571_cov_16.899736, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000541 DAOQMB010000000
VERSION     DAOQMB010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61427_length_1571_cov_16.899736"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1571)
                     /locus_tag="PLY05_11885"
     CDS             complement(<1..>1571)
                     /locus_tag="PLY05_11885"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNC04040.1"
                     /translation="LIRKVGTGLYNWLPLGLRVLQKVERIVREEMNRSNALEVLMPVT
                     QPAELWEESGRYVQYGAELLRFKDRHQANFVLGPTHEEVITDLARNELQSYKQLPVNF
                     YQIQTKFRDEIRPRFGVMRSREFIMKDAYSFHANQASLQETYDVMYDTYCRIFSRIGV
                     DFRPVQADTGSIGGDGSHEFHVLAESGEDLIAFSDTSNYAANIEMAEAICTSQRPTAS
                     QAMQSVDTPNAHSIAEVCEFLKVDASQTVKTLIVRGKADDKGQYGLVALVLRGDHELN
                     DIKAEKLAQVGSPLTFASEAEVLAAVGCHVGSIGPQGLTMPVLVDRSAAALADFVAGA
                     NVDGKHVTGLNWERDAQITAIVDIRNVVAGDPSPDGQGKLVLKRGIEVGHIFQLGKKY
                     SQALNCTVLGEDGKPFVVTMGCYGIGVTRVVASAIEQNYDERGIIWPDAIAPFHIALV
                     PMNWAKSQRIQEESQKLYEQLTAAGFDVFYDDRNERPGVKFADAELIGIPHRLVMGEK
                     GLDAGTIEYKGRRDS"
BASE COUNT          437 a          373 c          331 g          430 t
ORIGIN      
        1 tcactgtcac ggcggccttt gtattcaatc gtgcctgcat ctaagccttt ttcgcccatc
       61 actaaacgat gaggaatacc aatcaattcg gcatcggcaa atttaacccc cggtctttcg
      121 ttgcggtcat cataaaacac atcaaaccct gcggcagtta attgctcata cagtttttgg
      181 ctttcttctt gaatacgttg tgattttgcc cagttcattg gcactaaagc aatatggaaa
      241 ggtgcaatgg catctggcca aataatgcca cgttcatcgt aattttgctc aatggcagaa
      301 gcaaccacac gagtcacgcc aataccataa caacccatag tcacaacaaa cggtttgcca
      361 tcttcaccca acaccgtaca gttaagtgct tgtgagtatt ttttaccaag ttggaaaata
      421 tggccgactt caatgccacg ttttaacact aacttgcctt gaccatcagg tgatgggtca
      481 ccagcaacca cgttacggat atcgacaatg gcagtgattt gcgcatcacg ttcccagttt
      541 aagcctgtta catgtttgcc atccacattt gcacccgcaa caaaatcagc taaggcagca
      601 gcagagcggt caaccaacac aggcatagtt aaaccttgtg ggccaataga gccaacatga
      661 caaccaacag ccgctaacac ttcggcttcg ctagcaaagg ttaggggaga gccaacttga
      721 gctaattttt cagctttaat gtcatttaat tcatggtcgc cacgtaacac taaagccact
      781 aaaccatatt ggcctttgtc atcggctttg ccgcgtacaa ttaaggtttt gacggtttgg
      841 ctagcatcaa cctttaaaaa ctcgcacact tcggcaatgc tgtgggcatt gggcgtatca
      901 acgctttgca tcgcttggct tgcagtaggg cgttggctag tgcaaatggc ttcggccatt
      961 tcgatgttgg cggcatagtt agaggtatca gaaaaggcaa ttaaatcttc accgctttcg
     1021 gctaacacgt gaaattcatg tgagccatca ccaccaattg agccagtatc ggcttgaaca
     1081 ggacgaaaat cgacaccaat acgactaaaa atacggcaat acgtgtcgta catcacgtca
     1141 taggtttctt gtaaggacgc ttggttagca tggaaagaat aagcgtcttt cataataaac
     1201 tcgcgtgagc gcatcacgcc aaaacgtggg cgaatttcgt cacggaattt tgtttgaatt
     1261 tgataaaaat tcacaggtaa ttgtttgtag gattgcaact cattacgcgc taaatcggta
     1321 atgacttctt cgtgtgttgg gcctaagaca aagttagctt ggtggcggtc tttgaagcgt
     1381 aataattctg cgccatattg cacataacgg cctgattctt cccacaattc ggcaggttgg
     1441 gtaacaggca ttaacacttc taatgcgtta gagcggttca tttcttcgcg gacaattctt
     1501 tctacttttt gtaatacgcg taagcctaaa ggcagccaat tatataaacc agtgcccact
     1561 ttgcgaatta a
//
LOCUS       DAOQMB010000542         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_61429_length_1571_cov_12.874670, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000542 DAOQMB010000000
VERSION     DAOQMB010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61429_length_1571_cov_12.874670"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLY05_11890"
     CDS             <1..794
                     /locus_tag="PLY05_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04041.1"
                     /translation="SAKDRMFVRRPINKAILLSVGMSAAHYYVSGQVQFKTVAQGEEK
                     IQEIEQPKHLFGLEEDKVPEEPIDAWQICYGSVAGIDEEEKELPKVPTMTYMPYRVKA
                     VNRSPGGLKLVWTEPPPSSLRVGEVIGISEDRDKGWGVGVLHWIQQKSDKTIEIGIEL
                     LAAQPNACGVCLLKNNKPASDYMRGFLIPEMPSVEQPATLMTLNAGLTVGSVVRVSQY
                     GETVDISLTKLVLSTQSLSQFEFESVNKADDGFDPMASLWRKLEV"
     gene            complement(802..>1571)
                     /locus_tag="PLY05_11895"
     CDS             complement(802..>1571)
                     /locus_tag="PLY05_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC04042.1"
                     /translation="KDPLFVRQGTQMIPTPYTRQLIVPIQQALQSVEQSINQEPLFEP
                     LQSERHFCLGLRDVLEASILPPLLQHLQQHAPHICLSSVRLDRQDLESELTAGHIDFA
                     LEIPYAVSDSIEHLPVVSDQLVVVARQQHPALASGLNLENYLAQRHIMVSTRRKGWAL
                     EDVELNKAGYKRDIRLRCQHYFAACRVVSETDLLLTMPEQYARLANEQFGNQLFDFPL
                     KSPILDMHLYWHRSANKDPAHRWLREVLKQLFQQYTH"
BASE COUNT          464 a          279 c          364 g          464 t
ORIGIN      
        1 cttctgcaaa agatcgtatg tttgttcgtc gcccaattaa taaagcgatt ttgctaagtg
       61 tgggtatgtc ggctgcacat tattatgtca gtggccaagt acagtttaag actgtggctc
      121 aaggtgaaga aaaaatacaa gaaattgagc agccaaaaca tttatttggt ttagaagagg
      181 acaaagttcc agaagaacct attgatgcgt ggcaaatttg ttatggttcg gtagcaggta
      241 ttgatgaaga agaaaaagaa ttaccgaaag taccaaccat gacctatatg ccttaccgag
      301 ttaaggcagt gaatcgctca ccaggtggat taaaattggt gtggacggaa ccgcctcctt
      361 catcattacg tgtgggagag gttattggga ttagtgaaga tcgagacaag ggttggggcg
      421 ttggcgtatt acactggatt caacaaaaat ctgacaaaac tattgagatt ggcattgagt
      481 tgttagcagc acagcctaat gcttgtggtg tgtgtttatt aaaaaataat aaacctgcta
      541 gcgactatat gcgtggtttt ttaattcctg aaatgccttc tgtagagcaa ccagcgacgt
      601 taatgacgct taatgcaggt ttaaccgtag gtagtgtagt tcgtgttagt caatatggtg
      661 aaacggttga tatcagcctg acaaaattag ttttatctac gcaaagtcta agtcagtttg
      721 agtttgagtc agtcaataaa gcagatgatg gttttgatcc aatggcttct ttgtggcgta
      781 aattagaagt ttaaattgat gctaatgggt atattgctga aatagttgtt ttaacacttc
      841 tcgtaaccaa cgatgagcag ggtctttgtt agcactgcga tgccaatata agtgcatatc
      901 caatatgggg cttttgagag gaaaatcaaa aagttgattg ccaaattgct cattggccaa
      961 acgtgcatat tgttcgggca tggttaataa taaatcggtt tcactcacca cccgacacgc
     1021 ggcaaaataa tgttggcaac gtaaacgaat gtctcgttta tagcctgctt tattcagctc
     1081 tacatcttcc aatgcccaac ctttacgtcg tgttgatacc ataatatgac gttgggctaa
     1141 atagttttct aaatttaagc ctgaagctaa tgcagggtgt tgttgacgag caaccacaac
     1201 caattggtca ctgaccacag gtaaatgttc aatgctatca ctgacggcat aaggaatttc
     1261 taaggcaaaa tcaatatggc cagcggttaa ttcactttct aaatcttgtc ggtctaaacg
     1321 cacactcgat aaacaaatgt gcggcgcatg ttgttgtagg tgttgtaata acggcggcag
     1381 aatcgacgct tctaacacat ctcttagccc caaacaaaaa tgtcgttcgc tctgtaatgg
     1441 ttcaaataag ggttcttgat tgatactttg ttcaacactt tgtaaggcct gttgaatggg
     1501 gacaatcaat tgccgcgtgt agggtgtagg aatcatctgt gtgccttgcc gcacaaataa
     1561 ggggtctttg a
//
LOCUS       DAOQMB010000543         1567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_61735_length_1567_cov_11.162037, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000543 DAOQMB010000000
VERSION     DAOQMB010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61735_length_1567_cov_11.162037"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            399..>1567
                     /locus_tag="PLY05_11900"
     CDS             399..>1567
                     /locus_tag="PLY05_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005179022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNC04043.1"
                     /translation="MLGMLCAVGLGIYALSLDGTVRSKFEGKRWAIPAKVYSRPLELY
                     TGANLSKADILAELELLHYRQQANYDNAGVYSEQNGELYIHTRGFVFSDDTERSQVLK
                     LRFQGNTISDLASTQQNSKGIVRLEPLVIGGIYPKHNEDRVLMQLKDAPKYLQEALLA
                     TEDKNFYHHHGVSIRGTLRAIFVNLTSGELRQGGSTLTQQLVKNFYLTDERSLKRKVN
                     EALMAFLLEVHYDKKEILETYLNEVNLGQNGNRSINGFGLAAQFYFGQPISELSMPQI
                     ALLVGLVKGPSYYNPWRNPERALERRNVVLHNLLKDGHISQNQYEKAKAKPLGILKAP
                     TAGLSVFPAFLDVVRRQLRQEYQEDDLNSEGLRIFTTLEPRVQTVAQNAFNQQVA"
BASE COUNT          479 a          279 c          302 g          507 t
ORIGIN      
        1 ttatcatttc gcaatttttc taaatcacgt tgtagcattc gttgactgtt actagcttgt
       61 tcttgaggat aataatgagc aacagccgca taaatttcac tcagtttttt gtcgtgattt
      121 tgagtggcta agtaacgtaa cagatatttt tgtcttgtat tggcaggtat tttggtcatg
      181 gttattgtga gaacaacaaa taaaagtatc ttaaaaataa tttaaccata taacaatcct
      241 ctaaaacttc attcttgttt cacaaaaacg catcacattt gcttaaaact caggttacaa
      301 tgcaacaaat tttaaacttg taacaagtta tgactcgaca gcaactctct ctaaaatctg
      361 aacaaggttt tattttaacc attctgctat tgctgattat gcttggtatg ttatgtgccg
      421 tgggtttggg tatttatgcc ttatcgctag atggtacagt acgtagtaag tttgaaggca
      481 agcgttgggc tatccctgct aaggtttata gccgtccttt agagctatat actggcgcaa
      541 atctcagtaa agccgacatt ttggccgagt tagagttact tcattatcgc caacaggcca
      601 actatgataa tgcaggtgta tatagtgagc aaaatggtga gttatacatt catactcgcg
      661 gttttgtgtt tagtgatgat actgagcgtt cacaagtttt aaaattacgt tttcaaggca
      721 ataccatcag tgatttggct agtacccaac aaaatagtaa aggtattgtt cgtttagaac
      781 cactagtcat tggcggtatt tatcctaagc ataatgaaga ccgagtgcta atgcagctta
      841 aagatgcccc taagtattta caagaagcgt tattagccac cgaagataag aatttttatc
      901 atcatcatgg tgtatcaatt cgcggcacat tgcgtgctat ttttgtcaat ttaaccagtg
      961 gtgagttgcg tcaaggaggg agtaccttaa cccaacaatt agtcaaaaac ttctatttaa
     1021 ccgatgaacg ttccttaaaa cgcaaagtga atgaagcgtt gatggcgttt ttattagaag
     1081 tgcattatga caaaaaagag attttagaaa cctatttgaa cgaagttaac ttaggacaaa
     1141 atggtaatcg ctcgattaat ggctttggtt tggctgctca attttatttt ggccaaccaa
     1201 ttagcgaatt atctatgcct caaatcgccc tgttagttgg tttggttaaa gggccttctt
     1261 attataatcc ttggcgtaac cctgagcgtg ccttagagcg tcgaaatgtc gtcttgcata
     1321 atttattaaa agatggccat attagtcaaa atcaatacga aaaagccaaa gctaaacctt
     1381 tgggaatttt aaaagctccc actgcaggtt taagcgtgtt tcctgctttc ttagatgttg
     1441 tccgtcgtca attaagacaa gaatatcaag aagatgactt aaattcggaa ggtttaagaa
     1501 tttttaccac cttagaaccc agagttcaaa ccgttgccca aaatgcgttt aaccaacaag
     1561 tcgcaaa
//
LOCUS       DAOQMB010000544         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_62167_length_1562_cov_3.859987, whole genome shotgun sequence.
ACCESSION   DAOQMB010000544 DAOQMB010000000
VERSION     DAOQMB010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62167_length_1562_cov_3.859987"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLY05_11905"
     CDS             complement(<1..850)
                     /locus_tag="PLY05_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04044.1"
                     /translation="MVARKETNGTWTADFYQDKKRIRKKGFLTKSAVIRFENDTLATP
                     SEQNKQTDSLMDLVDAWYKLHGFGLKDAKYRYSRTKAIAERLGNPPAKDFNALAWANY
                     RTERLKTVTPETINHEQRYISAVFSELIRLGVWHESNPISAIRQIKTDQTELQFLSLD
                     QVKLLLSECQKSTNSHCYPVALLCLATGARWDEAESLTRQAVYGGKVHYHKTKNGQSR
                     AVPIKAEVEQLILESGLPSGRLFMSCRAAFRSAYERCGFDTPRQLTHILRHTFASHFM
                     MAGGDIL"
     gene            complement(850..>1562)
                     /locus_tag="PLY05_11910"
     CDS             complement(850..>1562)
                     /locus_tag="PLY05_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04045.1"
                     /translation="VHYHRAYGSRLIAQRKWQRCIDDPNEHHAIQEAEIKHLTLPKHI
                     FKEYKSTTIDTHKFKIPRKLVISLSVLLVLGGISAYLASGFLAKAADNVAKGKEDKKT
                     EQTTKPIENHQQNNTGLYSASLDNAKDRPLTIADFTPVTPTMPYTAPYYQDVAQPKTF
                     PRFAGCMRSFYKTKNQEICRCVTQQGTTLAVPLDQCAAVVDGDGMPFDPFREEQLAQS
                     SLNSSPAEHQTKQLGDRS"
BASE COUNT          426 a          306 c          341 g          489 t
ORIGIN      
        1 tcagtatgtc accgccagcc atcataaaat gactagcaaa tgtatgacgc aggatgtgcg
       61 ttagttgtct tggcgtatca aatccgcagc gttcataggc cgatctgaag gcagcgcggc
      121 aagacataaa taatcgtcca cttggtaagc cgctctcaag aattaattgc tctacttcag
      181 ctttaatagg cacagcccga ctttgaccat ttttagtttt gtggtagtga accttgccac
      241 cataaacagc ttgacgtgtg aggctttccg cttcgtccca tcttgcacct gtggcaaggc
      301 atagtaaggc gacagggtag caatggctat ttgtgctttt ttggcattca ctcaataaaa
      361 gcttgacctg atctaagctt aaaaactgca attctgtttg atctgttttt atctgacgta
      421 tagccgaaat agggttagat tcatgccaaa cacccaagcg aattaactca gaaaagaccg
      481 cagaaatata acgctgttca tggttaatgg tttcaggtgt caccgttttt aggcgttcgg
      541 tacgatagtt tgcccatgct aaagcattaa aatctttagc tggtggattg cctaaacgtt
      601 cagcaatggc ttttgtgcga gagtaacgat atttagcatc tttaagacca aaaccatgca
      661 atttatacca agcatcaacc aagtccatta aggagtcagt ttgtttattt tgttcgcttg
      721 gcgtggctag ggtgtcattc tcaaaacgga taacggctga ctttgttaaa aagccttttt
      781 ttctaatccg ctttttatct tggtaaaagt ctgccgtcca agttccgttg gtttctttac
      841 gtgcaaccat taactacggt ctccaagctg ctttgtttga tgttcggctg gtgagctgtt
      901 caaagatgat tgtgcgagtt gctcttctcg aaatgggtca aagggcatac catcaccatc
      961 aaccacggca gcacattgat ctaaaggaac ggctaaggtt gtgccttgtt gagtgacaca
     1021 acgacagatt tcttggtttt tagttttata aaacgagcgc atgcagccag caaaacgcgg
     1081 aaaggtttta ggttgtgcaa cgtcttgata ataaggcgca gtgtagggca tggttggcgt
     1141 tacaggcgta aaatctgcaa tggttagtgg acgatccttt gcattgtcta aggaagcaga
     1201 atacaaacct gtattatttt gctgatgatt ttctataggc ttagtggttt gttcggtttt
     1261 tttatcttct ttgcctttgg caacattatc agccgcttta gctaaaaatc ctgatgccag
     1321 ataagcagaa attccaccca gaaccaataa aacagataaa gatataacta atttacgcgg
     1381 tattttaaat ttatgtgtat caatggttgt ggatttatat tccttaaaaa tatgtttagg
     1441 tagcgttaaa tgtttaatct cagcttcttg tatagcatga tgttcgttag ggtcatcaat
     1501 acagcgttgc catttacgtt gagcaattaa acgtgaacca taagcccgat gataatggac
     1561 gt
//
LOCUS       DAOQMB010000545         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_62220_length_1561_cov_9.470120, whole genome shotgun sequence.
ACCESSION   DAOQMB010000545 DAOQMB010000000
VERSION     DAOQMB010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62220_length_1561_cov_9.470120"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLY05_11915"
     CDS             <1..238
                     /locus_tag="PLY05_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004659820.1"
                     /note="decatenates newly replicated chromosomal DNA and
                     relaxes positive and negative DNA supercoiling; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HNC04046.1"
                     /translation="AAENKRAKPQVTRFKGLGEMNPLQLRETTMDPNTRRLVQLTLDD
                     VKATESLMDMLLAKKRAADRKAWLEEKGNLAEVV"
     gene            571..>1561
                     /locus_tag="PLY05_11920"
     CDS             571..>1561
                     /locus_tag="PLY05_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04047.1"
                     /translation="MFLVHYIFWLSTLLLLQNQAYAAAIACNAVTEVSQTFSFSGTNT
                     WPSGSTTRNYTIGTAPNDVTLTFTNNQLVTAMPGDPTQRTHGNIANTVTISSNGGYAA
                     NTLLSTFALSTSRAINKLSFTITDEDSNTSSGNNFFDRTISTTNAGVTTAITADVGAN
                     QTINVGTGTATATSSADCDDTDNNCNIYLEFNRTGITSASTDFRAAHTGTSTTQRIGF
                     NQYAWCLPRMPTVKVQKITTGHTGAGGTFTFADTNLTGTIANISTTAPSTATPASPTA
                     LAVTTIDTDVTITETPAAGFTLSTATCSDANRASTGNPVSFGSLAGNVLTIPAT"
BASE COUNT          489 a          306 c          305 g          461 t
ORIGIN      
        1 agcggcagaa aacaaacgtg ctaaaccgca agtgactcgt tttaagggct tgggtgaaat
       61 gaatcctttg cagttgcgtg aaacaacaat ggaccccaat acgcggcgtt tagtacaact
      121 gactttagac gatgtgaaag caaccgaaag tttaatggat atgttattgg ccaaaaaacg
      181 tgcggctgac cgtaaagcat ggttagaaga aaaaggtaat ttggctgagg ttgtgtgata
      241 gtgttctctt tttgtcgtgt tttttagccc tttagtctga ttgttgtcaa aattgccacg
      301 ttttgtggca atttatactc tacaaaaata tacaactcca tcacattttt ctacaatcgt
      361 taattaattt ttatataaat ggcattgctt gctatgtggc tgttttgcca atgctaataa
      421 atgatgcaaa aaagtgcgct ataaccataa ataaagttgc gaaaacgctt ttattgctga
      481 acttatcacc tttattggtg gaaatagatg atgtcccaaa tgttgcttag atcacgtgtt
      541 atgtatctag ttaaaatcag aaaaaatgta atgtttttag tacattatat cttttggcta
      601 tcaacattat tgttgttgca aaatcaggct tatgcagcag caatcgcctg taatgcggtt
      661 acagaagtgt cacagacttt tagttttagt gggactaata catggccttc aggatcaaca
      721 actagaaatt ataccattgg tactgcaccc aatgatgtca ctttaacatt tacgaataat
      781 caattagtga ctgcaatgcc tggtgatcca acacaacgta cccatggcaa tattgccaat
      841 acagtgacca tttccagtaa tgggggttat gcagcaaata ctttgttatc tacctttgct
      901 ttatcaacca gtcgagccat taataaatta agtttcacca ttacggatga agattcaaat
      961 acttcatcag gtaataattt ctttgataga acaatatcaa ccacaaatgc aggggtaaca
     1021 acggctatta ccgctgatgt tggcgcaaat caaacaatta atgtgggtac aggaaccgca
     1081 acagcaacca gtagtgcaga ctgtgatgat accgataata actgtaatat ttatttggaa
     1141 tttaatcgga caggcatcac ctctgcaagc actgatttta gagcagctca tactggtaca
     1201 tccaccacac aacgtattgg ttttaatcaa tatgcgtggt gtttgccacg tatgcccaca
     1261 gttaaagtac aaaaaattac tacaggccat acaggtgcgg gtggtacgtt tacttttgcg
     1321 gatacaaatt tgacaggcac aattgcaaat attagtacaa ctgcccccag tacagcaaca
     1381 cctgcctcac caacagcctt agcggtaacg actattgata ccgatgttac cattaccgaa
     1441 accccagcag caggttttac tttaagtacc gcaacctgta gtgatgccaa tagagcatca
     1501 acaggaaacc ctgtgagttt tggttcgttg gcaggtaatg tgttaaccat tcctgcaacc
     1561 a
//
LOCUS       DAOQMB010000546         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_62224_length_1561_cov_6.163347, whole genome shotgun sequence.
ACCESSION   DAOQMB010000546 DAOQMB010000000
VERSION     DAOQMB010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62224_length_1561_cov_6.163347"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLY05_11925"
     CDS             complement(<1..234)
                     /locus_tag="PLY05_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04048.1"
                     /translation="MNNIKFIEGIGFIPNDWAVKKADELCSKITKGTTPPKEDIVNNS
                     HIPFLRVNNLTFDGVLDVNSGFIFVSEAAHRSVL"
     gene            complement(227..>1561)
                     /locus_tag="PLY05_11930"
     CDS             complement(227..>1561)
                     /locus_tag="PLY05_11930"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HNC04049.1"
                     /translation="GDEPELIAEMMKNERFVLPAHASFYALYEQRHEAGNGERIDKAL
                     HAIEETNGTKLKNVFQDISFNTDKLGEEKQKNTILRHILEDFAKPDMNLRPSRVGSLD
                     VIGNAYEYLIKNFAASGGQKAGEFYTPPEVSDLIATVLEPQSGDRICDPACGSGSLLM
                     KCGRKIRESTGKKEYALYGQEAIGSTWALAKMNMFLHGEDNHKIEWGDTIRNPKLLDK
                     NGDLELFDIVTANPPFSLEKWGHEEAEHDPFGRFRRGIPPKTKGDYAFILHMVETLRP
                     KTGRMGVVVPHGVLFRGSSEGAIRQKLIEENLLDAVIGLPEKLFYGTGIPAAILIFKK
                     SKADDKVLFIDASRHYKAGKNQNMLAPENIDKIVATYKARQSVDKYAYLATLQEIKDN
                     DYNLNIPRYVDTFEEEEEIDLLAVQTQRLVLKAELAVLEEKMAGYLKELGYE"
BASE COUNT          445 a          391 c          255 g          470 t
ORIGIN      
        1 taaaacactt ctatgagccg cctcactaac aaatataaaa ccgctgttta catcaagcac
       61 tccatcaaaa gttaaattat taacacgaag aaatggaata tggctattat taacaatatc
      121 ctcttttgga ggagttgtac cttttgttat ttttgagcaa agctcatcag cttttttcac
      181 tgcccaatca ttaggaataa aacctatccc ctcgataaat ttaatgttat tcataaccca
      241 actcctttaa ataaccagcc atcttctcct caagtactgc caactccgct tttaatacca
      301 aacgctgtgt ctgcactgcc aataaatcaa tctcctcttc ttcttcaaag gtatcaacat
      361 aacgcggaat attcaggttg taatcgttgt ctttaatttc ttgcaaagtg gctaaataag
      421 catatttatc aaccgattgc cgcgccttat aagtcgctac aatcttgtcg atattttcag
      481 gcgcaagcat attctggttt ttacctgcct tataatgacg gctggcatca ataaacaaca
      541 ctttatcgtc tgctttagat ttcttaaaaa tcaaaattgc cgcaggaata cccgtgccat
      601 aaaataactt ttcgggtaaa ccaatcaccg catctagcaa attttcttca atcagctttt
      661 gccgaatagc tccctcacta gagcctctaa acaacacacc atgaggcaca accacgccca
      721 tacgaccagt cttaggacgc aaggtttcta ccatatgcaa aatgaacgca taatcaccct
      781 tcgtttttgg cggaatacca cgtctaaaac ggccaaacgg gtcatgttca gcttcctcat
      841 gcccccattt ttctaaacta aatggcggat tagcggtaac aatatcaaat aactctaaat
      901 caccgttttt atccaataac ttcgggttac gaatggtgtc gccccactct attttgtggt
      961 tgtcttcgcc atgcaaaaac atattcattt tggctaatgc ccatgttgaa ccaatcgcct
     1021 cttgaccata caaggcatat tcttttttgc ctgtgctttc ccgaattttg cggccacact
     1081 tcatcaacaa cgaacccgaa ccacaggcag ggtcacaaat acggtcgccc gattgtggct
     1141 ctaataccgt tgctattaag tcggagactt cgggcggcgt ataaaattcc ccagcttttt
     1201 gtcccccact ggcggcaaag tttttaatta aatattcata ggcattgcca atcacatcta
     1261 aagaacccac acggctaggg cgtaagttca tatcaggctt ggcaaaatcc tctaaaatat
     1321 ggcgcaaaat agtgtttttt tgcttttctt cgcccagctt atcggtatta aaactaatat
     1381 cttggaacac gtttttgagc ttagtaccgt ttgtttcttc aatagcgtgt aaagctttat
     1441 caatacgctc gccgtttcct gcttcgtgac gttgctcgta caaggcgtaa aaactggcgt
     1501 gtgctggcaa cacaaaacgc tcgtttttca tcatctcagc aatcagctca ggctcatcgc
     1561 c
//
LOCUS       DAOQMB010000547         1559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_62381_length_1559_cov_7.318484, whole genome shotgun sequence.
ACCESSION   DAOQMB010000547 DAOQMB010000000
VERSION     DAOQMB010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62381_length_1559_cov_7.318484"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLY05_11935"
     CDS             complement(<1..656)
                     /locus_tag="PLY05_11935"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC04050.1"
                     /translation="MTPTISKITAIRLHEAGKLIQAQQMYQQLLETMPVDSDLWHRLG
                     ILYWQQNQFELAKNTLLQALNYQKKSPMLWLHYGVVLGSLNQHQLAQQSFESGLACQA
                     TATQQIELYYYHALALHKQHQLSHALNSIDKVLQLSPNHVQALNLQGTLYLQNHNLPQ
                     ALNCYQLAIKLKKDFAEAWYHCGIVLEKLHDYAKAINHYQQAICLKPHYVKALYNCAN
                     L"
     gene            789..1070
                     /locus_tag="PLY05_11940"
     CDS             789..1070
                     /locus_tag="PLY05_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04051.1"
                     /translation="MKYLSGLSIILFLIVNHPAFADKASAEMIVSARVISSCNIDSNQ
                     QQTTWSCSAKPSVNTDFTTIKTSIYSVSNQHQTLEKTELQTLIIYHVDF"
     gene            complement(1072..>1559)
                     /locus_tag="PLY05_11945"
     CDS             complement(1072..>1559)
                     /locus_tag="PLY05_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNC04052.1"
                     /translation="ATRDVTAWLKCEYMQDHIGDEFKGVISAVTAFGFFVSLEDIFVE
                     GLVHVRTLEGDYFNFDPISHVLLGSRTGISYAMGDKVSVKVAGVNLDERKMDFELLAK
                     LTSAKRSRSVKKEPVSEGKPSKKPRNKKPDLRSEAEKAKAKHKKAKVKSKAKRAKTSK
                     K"
BASE COUNT          473 a          304 c          264 g          518 t
ORIGIN      
        1 agattagcgc aattatataa agcttttaca taatgaggtt tcaggcaaat ggcttgttga
       61 taatgattaa ttgccttagc ataatcatgc aatttttcta gcacaatccc acaatgatac
      121 cacgcctctg caaagtcttt ttttagtttg attgccaact gataacaatt taatgcttgc
      181 ggcaaattat gattttgaag atatagagta ccttgtaaat ttaaagcctg tacatgattg
      241 ggtgacaact gcaatacttt atcaatgcta ttgagcgcat gacttagctg atgctgcttg
      301 tgtaaagcaa gtgcatgata ataataaagt tctatttgtt gtgtggctgt tgcttggcaa
      361 gccaaaccac tctcaaaact ttgctgagct aattgatgct gatttaatga gcctaaaaca
      421 accccataat gcagccataa cattggcgat tttttttgat aattcagtgc ctgcaacaac
      481 gtattttttg ccaactcaaa ctgattttgt tgccaatata aaatgcctaa acgatgccat
      541 aaatcactat caacaggcat agtttctaag agttgttgat acatttgctg tgcttgtatc
      601 agttttcctg cttcatgaag acgaattgct gttatttttg agattgttgg tgtcatggtg
      661 agcaatatgc catattttta taagaaaaac tgactaacgc atgtaaataa tacagaaaac
      721 ctaactaaca atatttttaa taccatatac actttaaata actctgaaga tcaacacgga
      781 tgttggccat gaaatattta agcggtttat ctataatcct ttttttaata gttaatcatc
      841 ctgcttttgc cgacaaagca tcagcagaga tgatagtgag tgccagagtc atttctagtt
      901 gtaatattga cagtaatcaa caacaaacaa cttggtcttg ctcagccaag ccctcggtaa
      961 acaccgattt tactactatt aaaactagca tttatagtgt gtctaatcag catcaaacat
     1021 tagaaaaaac tgagcttcag accttaatca tttatcatgt tgacttctaa gctatttttt
     1081 acttgtttta gctcgtttag ctttgctttt aactttggcc tttttgtgct tggctttagc
     1141 tttttcggct tcactgcgca aatctggctt tttgttacgc ggttttttgg agggtttgcc
     1201 ctcagataca ggttcttttt taacagagcg actgcgtttt gcactggtta acttagccaa
     1261 caattcaaaa tccattttgc gttcatctaa gttgacgcct gcaactttga cactgacttt
     1321 atcgcccatt gcataactaa tgcctgttcg tgaacctaac aacacatgag aaatggggtc
     1381 aaaattaaaa taatcgccct ctaaagtgcg cacatgcact aagccttcaa caaaaatatc
     1441 ttctaaagag acaaaaaagc caaaagcagt cacagcacta atcacgcctt taaactcatc
     1501 gccaatatgg tcttgcatgt attcacattt taaccatgca gtgacatcac gcgtggctt
//
LOCUS       DAOQMB010000548         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_63560_length_1544_cov_16.704500, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000548 DAOQMB010000000
VERSION     DAOQMB010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63560_length_1544_cov_16.704500"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..842)
                     /gene="uppS"
                     /locus_tag="PLY05_11950"
     CDS             complement(72..842)
                     /gene="uppS"
                     /locus_tag="PLY05_11950"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004830470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNC04053.1"
                     /translation="MSLAPDIDSHAGSLPKHIAIIMDGNNRWAKAKSLIGGAGHKAGE
                     SALQAIVEHAAKRGIQVLTVFAFSSENWRRPEQEVGLLMRLFVHALDKRVAELHKNNL
                     RIRFIGDRSRFAPLLQKGMNDAESKTANNTGMTLVIAVSYGGQWDMAHAAQQLALQAQ
                     QGKLDVAQLSMDDFQSIYQQQIQMSDLPPVDLMIRTGGDLRISNFLLWQTAYAEFYFS
                     SLLWPDFNEQALDDALSDYASRQRRFGRTSEQVESHHA"
     gene            complement(915..1469)
                     /gene="frr"
                     /locus_tag="PLY05_11955"
     CDS             complement(915..1469)
                     /gene="frr"
                     /locus_tag="PLY05_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNC04054.1"
                     /translation="MINDLKKDCEVRMKKSVDALDVAFAKVRTGRAHPAILKDIMVPY
                     YGMDTPLNQVANVGIEDARTLLVQPYERTMIQAIDKAIRNSDLGLNPLTGDVIRIPLP
                     ALTEETRRNMQKMARAEAENARVAVRNIRRDVLADVKELLKEKEISEDEERRATEEVQ
                     KITDKSIAEIEKALQAKEAELMQV"
BASE COUNT          446 a          347 c          276 g          475 t
ORIGIN      
        1 tgtgaccatt ggcgaaaaaa atacaccaaa tcacaatcgg gactaaaatt aaagcggtta
       61 atacacgttc cttaagcatg atgagactcc acttgctcac tggttcgacc aaaacggcgt
      121 tgacggctgg cataatcgct taaggcatca tctaaggctt gctcgttgaa atcaggccat
      181 aacaacgagc taaaataaaa ctcagcatag gccgtttgcc ataataagaa attactaatg
      241 cgtaagtcgc caccagtacg aatcatcaaa tcgacaggcg gtaagtcgct catttgaatc
      301 tgttgttgat atatgctttg aaaatcgtcc atcgacaatt gggcaacatc tagtttgcct
      361 tgttgtgctt gtaaagcgag ttgctgtgct gcatgcgcca tatcccattg gccaccataa
      421 ctcacggcta tcaccaaagt catgcccgta ttattggctg ttttactttc agcatcattc
      481 atgccttttt gtaataatgg tgcaaaacga cttctgtcac caataaaacg tattcgtaaa
      541 ttatttttgt gcaattcagc aacacgttta tctaaggcat ggacaaataa acgcattaat
      601 aaaccgactt cttgttcagg acgacgccaa ttttcacttg aaaaggcaaa gaccgttagc
      661 acttgtatgc cacgtttagc agcatgctca acaatagctt gtaaggctga ttcgcccgct
      721 ttgtgtcctg caccgccaat taagcttttg gcttttgccc aacgattatt accatccata
      781 ataatggcga tgtgttttgg caagctgcca gcatgactat caatatcagg tgctaaactc
      841 acaaaaaaac tccaataatc tacttaaata aaaggcggat tactccgcct ttttagtttc
      901 cacattgtgc aaaattatac ttgcatcaac tctgcttctt tagcttgtag agctttttcg
      961 atttcagcaa tagatttatc ggtaatcttt tgcacttctt ctgtggcacg acgctcttca
     1021 tcttcagaga tttctttctc ttttaacaat tctttcacgt ctgctaacac atcacgacga
     1081 atattacgca ctgcaacacg cgcattttcg gcttcagcac gtgccatttt ttgcatatta
     1141 cgacgcgttt cttcggttaa tgcgggtaat ggaatacgaa tcacatcacc tgttaaagga
     1201 tttaagccta aatctgagtt acgaatcgct ttatcaatcg cttgaatcat ggtgcgttca
     1261 taaggttgta ccaataaggt acgtgcatct tcaataccta cgttagcgac ctgatttaaa
     1321 ggtgtgtcca taccataata tggcaccata atatccttta aaatagcagg atgtgcgcga
     1381 cctgttctga ctttagcaaa agcgacatct aaggcgtcta cacttttttt catgcgaact
     1441 tcgcaatctt tttttaaatc attaatcatg tgaaaattcc ttaacgaggt ttattgccaa
     1501 ttaatgtgcc ttcactttga ccacaaacaa ggtttaataa tgca
//
LOCUS       DAOQMB010000549         1544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_63561_length_1544_cov_16.345198, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000549 DAOQMB010000000
VERSION     DAOQMB010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63561_length_1544_cov_16.345198"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1101
                     /locus_tag="PLY05_11960"
     CDS             199..1101
                     /locus_tag="PLY05_11960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNC04055.1"
                     /translation="MKFPLWRQYAMRPLMLRLTPATIATFLGTVGLLASAVGAVGYAV
                     GTKNTEPVKHSVVSQQSTVSTTQPEQQLQAVNQRVAEMQAQLLRLDALTQQMAQSAHV
                     PVQEFTIDPKGAKGGPLLEEMSVLGEDNIDLRLQELSTLIAQKEAQIKALDRILSQKK
                     WQSNLNYLANLPVRQGAITSTYGYRTDPFTGRTAFHAGMDFSGAEGEDIYAVAAGIVS
                     FAGQKSGYGNVIEVTHGDGYITRYAHAQRLAAKEGDMVAKDQVIAYMGSTGRSTGTHL
                     HYEVLVNGKQIDPMTYVSMALKRD"
     gene            1354..>1544
                     /locus_tag="PLY05_11965"
     CDS             1354..>1544
                     /locus_tag="PLY05_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004919773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04056.1"
                     /translation="MFARLISGIFGTKNERELKRMQKMVDAINALEPSIQALDDAALS
                     AKTQEFRDRYTKGETLDQL"
BASE COUNT          481 a          276 c          326 g          461 t
ORIGIN      
        1 ttgattatgg cacagaagtg tcgattgtct aaatctaaaa gcaagttatt gcatagaaat
       61 gacaatataa caggtgaatt atcaataaag actactctgc gaatcactaa gctatatagt
      121 tttatggctt agttcaattg ccaagagagc ctttagtcaa ataatgatta gaggctaagt
      181 aagcaggaga taaacagcgt gaaatttcct ttatggcgtc aatatgccat gcgacctttg
      241 atgttgcgtt tgacccccgc taccatagcg acctttttag gaacagtggg tttgttagct
      301 tccgccgttg gtgcggttgg ttatgccgta ggtactaaaa atacggaacc tgtaaaacat
      361 tcagttgtta gtcaacaatc gactgtttca acgacacaac ccgaacaaca attgcaagca
      421 gtcaatcaac gtgttgccga aatgcaagca caattattgc gtttagatgc tttgacacaa
      481 caaatggcgc aatcagccca tgtgcctgtt caagagttta caattgatcc taaaggggca
      541 aaaggtggcc ctttattaga agagatgtcg gttttaggtg aagataatat tgatcttcgt
      601 ctgcaagagt tatcgacatt aattgctcaa aaagaagctc aaattaaagc ccttgatcgt
      661 attttgtcgc aaaaaaaatg gcaaagtaat cttaattatt tagcgaatct accagttcgt
      721 cagggtgcaa ttacctctac ttatggttat agaacagatc cttttacagg acgtactgct
      781 tttcatgcgg gcatggattt ttctggagca gaaggtgaag acatttatgc agttgccgca
      841 ggtattgtta gttttgcagg acaaaaatct ggctatggta atgttattga ggtaacacat
      901 ggtgatggtt atattacacg ttatgctcat gcccaacgtt tagcggccaa agaaggcgat
      961 atggttgcta aagatcaagt gattgcctat atgggatcaa caggtcgttc aacaggtact
     1021 catttgcatt atgaagtgct tgtcaatggc aaacaaattg atccaatgac ctatgtcagc
     1081 atggcattaa aaagagacta attttttagt ttagtcataa aaataagcca atagagatat
     1141 tggcttattt ttttgtcagt attttgcagt gaatcccaaa aagctacttc tcaagcgtgt
     1201 aattctaggt agaatcacgc cctgcacagg atatgtttca aattttacaa gcgtgagtct
     1261 aaaacgcttg tctttgctag ttttgccccc atctctatgc acaacagcct agctgataca
     1321 aaaagagtgg atatttaaat aagagtggct tttatgtttg cacgcctgat tagcggaatt
     1381 tttggcacga aaaacgaacg cgaattaaaa agaatgcaaa agatggttga tgccatcaat
     1441 gctttagaac ccagtattca agctttagat gatgcggctt tatccgctaa gactcaagag
     1501 tttcgtgacc gctatactaa gggtgaaact ttagatcaat tatt
//
LOCUS       DAOQMB010000550         1542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_63713_length_1542_cov_38.199731, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000550 DAOQMB010000000
VERSION     DAOQMB010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63713_length_1542_cov_38.199731"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..1155)
                     /locus_tag="PLY05_11970"
     CDS             complement(253..1155)
                     /locus_tag="PLY05_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC04057.1"
                     /translation="MKIGYARVSTREQNPSLQIDALKQAGCERIFQDVASGAKSSRPE
                     LDKLLAEARTGDTIIIWKLDRLGRSLRHLVELVETLSGQNIGLKSLNDPVDTTHAQGR
                     LIFNLFASLAEFERDLIRERTNAGLSAARARGRVGGRPKGLDAQAEATAMAAETLYRE
                     GRLSVNAIADKLHISKSTLYSYLRHRGVEIGAYQKAVTTTRENPSVEQVADITLRLTV
                     ENNSQFVRGKKRARENIERYCLDKYHATRLSSGDYSLRINYKTREDLDVLMADLLRDM
                     SDEAEMRHCYIEADAWEQGTEYRW"
BASE COUNT          459 a          332 c          271 g          480 t
ORIGIN      
        1 ttgtttaatc tgtgcttcaa cacgcgcact gtagtaggtg ataatatagc tcatgtcgtt
       61 aagttagcaa atatgagaac tttatgcaat ctaaattatt tgacaacgcc aaggtaaatt
      121 aacaccctta gcttgtttta ttgagtgtaa agtttaagct taaagtattt gggtgtattt
      181 aagactgaaa agtagcatag ggttttgtga gcaatggaac ggcgaaccga cttaacatac
      241 accccctaaa actcaccacc gatactccgt cccctgctcc cacgcgtccg cctcaatata
      301 acaatggcgc atctctgcct catcactcat atcacgcaat aaatcagcca ttaatacatc
      361 taaatcttca cgggttttat aattgatgcg taagctataa tcaccagacg ataaccgtgt
      421 tgcatggtat ttatctaaac aataacgctc tatattttcc cttgctcgtt ttttaccgcg
      481 tacaaactga ctgttatttt caacagtcaa gcgcagcgta atgtcagcta cttgctcaac
      541 tgatggattc tccctcgtag ttgtcaccgc tttttgataa gcccctattt cgacaccacg
      601 atgtcgcaga tagctgtata aggtactttt agaaatatgc aatttatcgg caatcgcatt
      661 gacactcaat cgcccttctc gatacaaggt ttccgcagcc atcgccgttg cttccgcttg
      721 tgcatctaaa cctttgggtc gtccccctac cctgccacgc gctctggcag ccgacaaacc
      781 tgcattggta cgttctcgga ttaaatcacg ctcaaactct gctaacgagg caaatagatt
      841 aaaaatcagt cggccttgcg catgtgtggt atccacaggg tcatttaaac ttttaagacc
      901 aatgttttgg ccagacaagg tttcaactaa ttcaactaaa tggcgaagtg aacgtcctaa
      961 acgatccagc ttccaaatga tgatagtgtc acctgtccga gcttctgcta ataatttatc
     1021 taactcagga cgactacttt tagccccact cgccacatct tgaaaaatac gctcacagcc
     1081 tgcttgttta agggcatcta tttgcaagga gggattttgt tcacgggtac tgacacgggc
     1141 atagcctatt ttcatgaaat agtcctgttt actcaatgag tattagataa attaaacttt
     1201 gattaactag acaagtattt aagactataa cagggttaaa ttcaaggatt atactcagat
     1261 tcagtctaaa ccaataaagt tcaataaaac gttcctttta tcgaactata aataacgcaa
     1321 ttttcgtgtg atgataaaat gaagatggtg ggacatgtta agaaaacttc acttttctta
     1381 acatgtttgc tttttagaca gcaaataaac agttaactta gagagtgtat attttacata
     1441 ctctttttta tagctatgat cttagccaat cgtttagaca gtatttttat ctaaatgttg
     1501 tatatgattg atcaaatgat ttaggttttc gaagtcaaaa aa
//
LOCUS       DAOQMB010000551         1538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_64039_length_1538_cov_15.275792, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000551 DAOQMB010000000
VERSION     DAOQMB010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64039_length_1538_cov_15.275792"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1504
                     /gene="terL"
                     /locus_tag="PLY05_11975"
     CDS             89..1504
                     /gene="terL"
                     /locus_tag="PLY05_11975"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNC04058.1"
                     /translation="MSNLQLSRQAAAFELLARRKAKESLIDFTKYTNDLYRPAPHHEL
                     IAKKLEQVAKGEITRLIICMPPRHGKSELASRRFPAWYLGRYPSEQIIAASYNSDLAN
                     DFGREVRNIVNSPDYRNVFDVELSQDSKAANRWHTNKQGMYVAAGVGTAVTGRGANIL
                     LIDDPFKDRQDADSELQRQRVWDWYTSTAYTRLMQKGAIVVINTRWHDDDLTGRLLAA
                     QQNGGDKWEVLSLKAIDDDGNALWPSDYPIERLEQIKGVLPPRDWNSLYQQDPIPDDG
                     EYFKKEWFIEYEKLPSELTYFAASDYAVTDGNGDFTEHGIFGLDHSGNLYLVDWWYGK
                     TTTDVWIDAKCDLIIKYKPDCWFGESGVIRRSIEPFLIRRMTERNAMCRIEWIASVSD
                     KESRARGFQALASMGKIHVPKNAMYKDHVMSQLIRFPAAKHDDAVDVCSLVGRGLEFI
                     NVRQAPKPYKRQNYGGHGWMG"
BASE COUNT          472 a          261 c          367 g          438 t
ORIGIN      
        1 cttaactcaa acattattgc tcgtgaatta ggtttagcag ataaacaaca agccgaaatt
       61 aagattactg atgtaaaaga gctaacaaat gagcaacttg caattatcgc ggcaggcggc
      121 agcatttgag ctactcgcta gacgaaaagc taaagagtca ttaatcgatt ttacaaagta
      181 cactaatgat ttataccgcc ctgctcctca ccatgaattg attgcaaaaa aactagagca
      241 agttgcaaaa ggtgagatca cacgcttaat tatctgtatg cctccgcgtc atggtaaatc
      301 cgaattggct tctaggcgtt tccctgcctg gtatttgggg cgatacccaa gcgaacaaat
      361 tattgcagct tcttataact cagacttagc aaatgacttt ggccgagaag ttagaaatat
      421 cgttaattcg cccgattata gaaatgtatt tgatgttgaa ttatcgcaag attctaaagc
      481 ggctaaccga tggcacacaa ataaacaagg catgtatgtc gcggctggtg ttggcactgc
      541 tgtaactggt cgtggtgcaa acattttact aattgatgac cctttcaaag accgtcaaga
      601 tgccgatagc gaattacaaa ggcagcgcgt ttgggattgg tacacgtcaa cagcttatac
      661 gcggttaatg caaaaaggcg caattgttgt catcaatacc cgatggcatg acgatgattt
      721 aacaggtaga ttattagcag cgcaacaaaa cggaggcgat aaatgggagg tgctgagttt
      781 gaaggctatt gatgatgatg gaaatgcttt atggccaagc gattatccta ttgagcgact
      841 tgaacaaatt aaaggtgtgt tgcctccgag agattggaat agcctttatc agcaagaccc
      901 catacctgac gatggtgaat atttcaaaaa agaatggttt attgaatatg aaaaactgcc
      961 aagcgagcta acctattttg ctgcaagtga ctacgcggtc acggacggca atggcgattt
     1021 taccgagcat ggtatttttg gattagatca ttcgggtaat ttgtatttgg ttgattggtg
     1081 gtatggcaaa acaacaacag atgtttggat tgacgctaaa tgtgatttga ttatcaaata
     1141 taagcctgat tgttggtttg gtgaatcagg tgttattagg cggtcaattg aaccgttttt
     1201 aattagacga atgacagagc gtaatgcaat gtgtcggatt gagtggatcg ctagtgtttc
     1261 tgataaagaa tcaagggcta gaggttttca ggcgttggca agcatgggca aaattcatgt
     1321 tccaaaaaat gctatgtata aagatcatgt tatgtcacaa ttaattagat tccctgcggc
     1381 caaacatgat gatgctgtcg atgtttgctc attggttggt cgtggtttag agtttattaa
     1441 tgtgagacaa gcaccaaaac catataagcg acaaaattat ggcggtcatg ggtggatggg
     1501 gtgaattatg ggcagtttaa gagattttct aattgatg
//
LOCUS       DAOQMB010000552         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_64125_length_1537_cov_6.886640, whole genome shotgun sequence.
ACCESSION   DAOQMB010000552 DAOQMB010000000
VERSION     DAOQMB010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64125_length_1537_cov_6.886640"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLY05_11980"
     CDS             <1..839
                     /locus_tag="PLY05_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC04059.1"
                     /translation="GLEVTSKGDFIFYALMNVPLYLALALLTWRLSPEGLKLLSAQHA
                     LIAYAVIAALAIYQWTKIWHVNKHLFTGEHPAAPSYKFKQVAILNLAYMACFGSELAV
                     VSMLPLFFIDTFHVSPVYAGMTAGCFAVMNLFARPSGGLFSDAYGRRKMLVICLLGQT
                     IGYAAMSQMGSEWSIVMAIATVLATSVFVQGACGAVYSVVPLIQRRMTGQIAGMAGAY
                     GNVGGVTFLTVLSMVSPSTFFWVLAGISALALASAFFLDEPKGHMVEKDEHGNVHLIA
                     VE"
     gene            1044..>1537
                     /locus_tag="PLY05_11985"
     CDS             1044..>1537
                     /locus_tag="PLY05_11985"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC04060.1"
                     /translation="MNHQLTVKAAQLSHQGPKASNDDAIGLHLPQGHGLASQTLAVAI
                     ADGLSSAEGGRVAAETSVTNFLNDFFATPASWSIKTSGFKVLEALNRWLHGQGQHYHH
                     HHKAHLTTFTALVIQGRTAHVFHIGDSRLWRLRGEDWECLTNDHALSTRHGNQLTRAL
                     GMDWR"
BASE COUNT          393 a          305 c          354 g          485 t
ORIGIN      
        1 gtggcttaga agtaacgagc aaaggcgatt ttatttttta tgccttaatg aacgtaccgt
       61 tatatttagc cttagcctta ttaacatggc gtttgtcgcc cgaaggccta aagttattat
      121 cggctcaaca tgcgttaata gcttatgcag taattgctgc cttagccatt tatcaatgga
      181 ctaaaatctg gcacgttaac aaacacttgt ttacaggtga acaccctgct gcaccaagct
      241 ataaatttaa acaagtggct attttaaact tagcatacat ggcgtgtttc ggttctgagt
      301 tagctgttgt gtcgatgtta ccgttgttct tcattgatac tttccacgtc agtcctgtgt
      361 atgcaggcat gaccgcaggc tgttttgcgg tgatgaactt gtttgctcgc cccagtggtg
      421 gtttgttttc tgatgcttat ggccgtcgta aaatgttggt tatctgctta ttaggtcaaa
      481 ccattggtta tgccgctatg tcacaaatgg gtagcgaatg gtcgattgtc atggcaattg
      541 ccactgtctt agcaacttct gtttttgtgc aaggggcttg tggggcggtg tattctgttg
      601 tgccgttaat tcaacgccgt atgacaggtc aaattgcagg catggcaggt gcttatggta
      661 atgtcggtgg tgtaactttc ttaactgttt tgagtatggt tagcccaagc accttttttt
      721 gggtgcttgc tggtatttcg gcattagctt tagccagtgc attcttctta gatgaaccta
      781 aaggtcacat ggttgaaaaa gacgagcatg gaaatgttca ccttattgcc gtagagtaaa
      841 agtgagttgc ttttagtata ggacttacgc ggttatcgct tatttgctca ctaaagaatc
      901 gttttcaacg attgagagtt cacaaaacgc gctaatttcg atgaaatgcg taagtcctga
      961 gcaatcaaag cagacaagga ggtctgcttt tttattaggc acagtttgtg tatatcaaac
     1021 ataaattatt gagcttttaa gcgatgaatc accagctaac ggttaaagca gcacaattat
     1081 cacatcaagg gcctaaggct agtaatgacg atgccattgg cttacatctt cctcagggtc
     1141 acggtttagc aagtcaaacc ttagccgttg ccattgctga tggcctaagc agcgccgaag
     1201 gtggtcgcgt tgccgccgaa accagcgtga ccaattttct aaacgatttt tttgctacgc
     1261 cagccagttg gtcaatcaaa acatcaggct ttaaagtctt agaagcactt aatcgttggt
     1321 tacatggcca aggtcaacat tatcaccatc atcataaagc gcatctaacc acttttactg
     1381 ccttagttat tcaaggaaga acggctcatg tatttcatat tggtgatagt cgtttgtggc
     1441 gtttacgtgg cgaagactgg gaatgtttaa ccaacgatca cgcattaagc acacgtcatg
     1501 gcaatcaatt aacacgtgct ttaggtatgg attggcg
//
LOCUS       DAOQMB010000553         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_64132_length_1537_cov_5.320513, whole genome shotgun sequence.
ACCESSION   DAOQMB010000553 DAOQMB010000000
VERSION     DAOQMB010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64132_length_1537_cov_5.320513"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1225
                     /gene="rlmM"
                     /locus_tag="PLY05_11990"
     CDS             176..1225
                     /gene="rlmM"
                     /locus_tag="PLY05_11990"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: protein motif:HMM:NF008734.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HNC04061.1"
                     /translation="MTNAYLIYCRAGFEKEATAELQHFATQYDWQGYVKSKADSAFVL
                     FCGQDLPTTGKQAPQWRDLVFARQLLFLKHSEPLKLNAEDRISPLIEACDGQFYQDVV
                     FEGADTNDGKSLAGFFKSFAPAFSSVLKKRTLLRKKAEQRLHLFFLDSTTVYIGYSQM
                     NNSSPWPMGVPRLRFPKDAPSRSTLKLEEAFHYYLDEQTQQEWLREGMTGVDLGAAPG
                     GWTWQLVDRGLQVTAIDNGPMDKALMQSGQVEHLKVDGFKYRPERPVTWLVCDMIESP
                     ARVAQTIASWIADGDAQKAIFNLKLPMKKRFEELLHCQDLMDGILSAKNVNYRLAFKQ
                     LYHDREEVTGYLYRV"
     gene            1321..>1537
                     /locus_tag="PLY05_11995"
     CDS             1321..>1537
                     /locus_tag="PLY05_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04062.1"
                     /translation="MTTSAVNWFEIPVINLERATAFYQNLLATELRIEQCSGMELAVF
                     PYEQEKGIGGALMAMPEHSPNTHGSIVY"
BASE COUNT          474 a          284 c          327 g          452 t
ORIGIN      
        1 taggagaaca tcgctaccgt gtggttttac taatgacata aaaattcctc taaaacttgc
       61 agagcaataa aaaggcggta attatacgcg tttttagtga taacttaact gataatcact
      121 tattagttaa atcaataagt gatttcaaca attctgaata tggactaaga ccacaatgac
      181 caatgcttat ttaatctatt gtcgtgcagg ttttgaaaaa gaagcgacag ccgaattaca
      241 acattttgcc acgcaatatg attggcaagg ctatgtaaaa agcaaagccg acagtgcttt
      301 tgtcttattt tgtggccaag atttacccac aacaggtaaa caagcaccac aatggcgtga
      361 tttagttttt gctcggcaat tattattttt aaaacacagt gagcccttaa aactgaatgc
      421 agaagatcgt attagcccat taattgaggc atgtgatggt cagttttatc aagatgtggt
      481 gtttgaaggg gcagatacca atgacggtaa aagtttagca ggctttttta aatcttttgc
      541 acctgcgttt agctcggtat taaaaaaacg tactttatta cgcaaaaaag ccgaacaacg
      601 tctgcatttg ttttttttag actcaaccac cgtttatatt ggctatagcc aaatgaataa
      661 tagttcgcct tggccaatgg gcgtaccacg tttacggttt cctaaagatg cccccagccg
      721 ctctaccctc aaacttgaag aagcctttca ttattattta gatgaacaaa cgcaacaaga
      781 atggctacgt gaaggcatga cgggcgttga tttaggtgca gcacccggtg gttggacatg
      841 gcagttagtt gatcgtggtt tacaagtcac cgctattgat aatgggccga tggataaagc
      901 cttaatgcaa tctggacaag tagaacactt aaaagtggat ggctttaaat atcgccctga
      961 acggcctgtc acttggttgg tgtgcgatat gatcgaaagc cctgctcgtg ttgcccaaac
     1021 cattgccagt tggattgccg atggtgatgc gcaaaaagca atttttaatt taaaactgcc
     1081 gatgaaaaaa cgttttgaag aactattgca ctgccaagat ttaatggatg gcattttatc
     1141 ggccaaaaac gtcaattacc gtttagcgtt taaacaactt tatcatgatc gtgaagaagt
     1201 gactggctat ttgtatcgtg tctaaaaaat actactgaca caatgctgtc agtaggggga
     1261 tgttgtaatg gtgttgggct gaatcaagtc ctgtaaaaaa tacttatgga gcaaagcaac
     1321 atgacaacat ctgccgttaa ctggtttgaa atccctgtga tcaatttaga gcgtgctacc
     1381 gctttttatc aaaatttatt ggcaacagaa ctacgtattg aacaatgtag tggcatggaa
     1441 ttagccgttt ttccgtatga gcaagaaaaa ggcattggcg gtgcattaat ggcaatgcct
     1501 gagcatagtc ctaatactca tggctcaatt gtctatt
//
LOCUS       DAOQMB010000554         1535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_64288_length_1535_cov_18.472973, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000554 DAOQMB010000000
VERSION     DAOQMB010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64288_length_1535_cov_18.472973"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="PLY05_12000"
     CDS             complement(<1..683)
                     /locus_tag="PLY05_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HNC04063.1"
                     /translation="MSLKTHLQAWSDLFGRYKQIFAIAWQNRHETDSKGLQPHEAEFL
                     PAALSLQETPVSPAPRVAMWLLISFTVIALLWACFGKIDIVATAQGKIVPNAGTKIIQ
                     PFELSTIKAIHVKDGQQVKAGDVLIELDATTTQADKDRVSSELALNRLQEARAQAMLK
                     ALEQQQTPVLLRPAQVTESQFVEADALLQGQYKEYQSKLALLDADIITKQAEQRSLQE
                     QIKSIEKSLP"
     gene            complement(690..>1535)
                     /locus_tag="PLY05_12005"
     CDS             complement(690..>1535)
                     /locus_tag="PLY05_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC04064.1"
                     /translation="LAQLWTDFQQTGISIQRLGDILNTRTEIANSKTALPPIRGQIQL
                     DQVVFRYRPDMPEVIKNMTLGVNAGEVIGVVGRSGSGKSTITKLIQRMYLPEKGRVLI
                     DGLDIALADASSLRRQIGVVLQENVLFNRSIRENIALADPSIPLPVVMQAAKLAGAHE
                     FITELPEGYDTLVGEHGSSLSGGQRQRIAIARALITNPRILIFDEATSALDYESERII
                     QQNMKAICKGRTVIIIAHRLSAVRDANRIIVVEKGQIIEQGTHTELLANPKGMYSHLH
                     SMQQG"
BASE COUNT          427 a          332 c          302 g          474 t
ORIGIN      
        1 ggtaaacttt tttcaatgct tttaatttgc tcttgcagcg aacgctgttc cgcctgttta
       61 gtaataatat cagcatctaa taaagctaat ttgctttgat attccttata ttgaccttgc
      121 aataaagcat ctgcctcaac aaattgactt tcagtgactt gtgcgggacg taacaacaca
      181 ggggtttgtt gttgctctaa ggctttaagc atggcttgtg cacgtgcttc ttgtaagcga
      241 tttaaagcta attcactgct cactcggtct ttatctgctt gtgtggtggt ggcatctaac
      301 tcaattagca catcacccgc tttaacttgt tgaccatctt ttacatgaat tgctttaatc
      361 gttgataatt caaagggctg gattattttt gtccctgcat tgggtacaat ttttccttgt
      421 gcggtggcaa caatatcaat tttgccaaaa cacgcccaca ataaagcaat aacagtaaaa
      481 ctaattaata accacattgc cacgcggggt gcaggtgaca ctggtgtttc ttgtaaagac
      541 aatgcagcag gtaaaaactc ggcttcgtgg ggttgtaaac ctttgctatc ggtttcgtga
      601 cgattttgcc aagctatcgc aaaaatttgt ttataacggc caaataaatc tgaccatgct
      661 tgtaaatggg ttttaagact catggtgatt tatccttgct gcatactgtg taaatggcta
      721 tacatacctt tgggattagc taataactca gtatgtgttc cttgttcaat gatttggcct
      781 ttttcaacaa caataatccg gttagcatca cgcactgccg ataaacgatg ggcaatgata
      841 attaccgttc tgcccttaca aatggctttc atattttgtt gaataattcg ttctgattca
      901 tagtccaaag cactggttgc ttcatcaaaa atcaaaatcc gtggattggt aattaaggca
      961 cgagcaatag caatacgctg acgttggcca cctgataagg atgaaccatg ctcacccact
     1021 aaggtgtcat aaccttcagg taactcggta ataaactcat gtgcacctgc caatttggcg
     1081 gcttgcatca ccacaggcaa gggaatactt gggtcagcta aagcaatatt ttcccgaatg
     1141 ctacggttaa acaacacgtt ttcttgcaac accacaccaa tttggcggcg taatgaagag
     1201 gcatcggcta aggcaatatc taaaccatca attaacactc gtcccttttc gggtaaatac
     1261 atacgctgaa tcagtttggt aatcgtgctt tttcctgatc ctgaacgacc aaccacacca
     1321 attacctcac cagcattcac gcccaaggtc atatttttaa tcacttcggg catatctgga
     1381 cgatagcgaa acaccacttg atcgagttga atttggccac gaattggtgg taaagcggtt
     1441 ttactgttcg caatttcggt acgggtattt aaaatatccc ccaaacgttg aatcgaaatc
     1501 cctgtttgtt gaaagtctgt ccataattgg gcaag
//
LOCUS       DAOQMB010000555         1532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_64544_length_1532_cov_17.206500, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000555 DAOQMB010000000
VERSION     DAOQMB010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64544_length_1532_cov_17.206500"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..683)
                     /gene="slyD"
                     /locus_tag="PLY05_12010"
     CDS             complement(198..683)
                     /gene="slyD"
                     /locus_tag="PLY05_12010"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC04065.1"
                     /translation="MMQISNDNVVSIAYTLTNDEGQVIDTSEGGAPLVYLHGAQNIIP
                     GLENALTGKQVGDKLKVSVEPAEGYGEYQAELVQVVPKRMFAGVDNIEAGMQFHAQTD
                     YGMQIVTVAAVEGEEITVDGNHPLAGQNLHFEVEVVDVRAASKEELEHGHVHGEGGHH
                     H"
     gene            complement(816..>1532)
                     /locus_tag="PLY05_12015"
     CDS             complement(816..>1532)
                     /locus_tag="PLY05_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011182844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC04066.1"
                     /translation="SHEFARYGLDDIQPQNVKITLIEAADRVLPVLPPQVSAEAEVQL
                     KAKGINVLTKTKVLSIQAHYVECEGAQRIPADLKVWSAGIKAPDFLQNIAGLETNRIN
                     QLVVKPSLQTTRDNNIFAFGDCASCTLPNMERPVPPRAQTAHQQATYLVKALAAHIQN
                     KPLPVYKYSDFGSLVSLSHGSSVGNLVGNIHVQGKIARLMYVMLYRMHQYALHGWFRT
                     FLLWAKDQLNKNASPTLKLH"
BASE COUNT          457 a          321 c          317 g          437 t
ORIGIN      
        1 ttaaccgtct ctagtaagga agcagaacgt cggcaactag aggaagaaat ggagcgtttc
       61 ttggcagcag gtggaaagat tcgtcagatt cctgccgaag agagcaatga tccgccaaaa
      121 cgaccacgca gtagttatgg ctctaaacct atataatttt aataaaaaag cctcgtaaaa
      181 cgaggctttt ttatggctta gtggtgatga ccaccttcgc catgaacatg accatgttct
      241 aattcttctt tactagcagc acgaacatca accacttcaa cttcaaaatg taaattttgg
      301 ccagctaaag gatggttgcc gtcaacagtg atttcttcac cttcaactgc agcaacagtc
      361 acgatttgca taccatagtc tgtttgagca tggaattgca taccagcttc aatgttgtct
      421 acaccagcaa acatacgctt aggtacaact tgtactaatt cagcttggta ttcgccataa
      481 ccttcagcag gctcaacaga tacttttaat ttgtcgccaa cttgtttgcc agttaaggca
      541 ttttctaaac caggaataat gttttgtgcg ccgtgtaaat acactaatgg tgcgccacct
      601 tcggaggtat caatgacttg gccttcgtca ttggttaagg tgtaggcaat agagactaca
      661 ttgtcattag aaatttgcat aataaaaacc ttttaggcac ggaggccgtg agaagaaagg
      721 ggtatttgtt aaaacaaata ccgccccaaa tttgaccgat gataatactg caattgctac
      781 attatgcgta ctaaaaaatg tagcttttat gaatcttaat gcagttttag cgttggactt
      841 gcatttttat tgagctgatc ttttgcccat aacaagaaag ttctaaacca accgtgtagc
      901 gcatattggt gcatacgata cagcatcacg tacatcaaac gggcaatttt accttgaaca
      961 tgaatattac ccaccaaatt acccacgcta ctgccatgac ttaaagacac taaagaacca
     1021 aagtcactgt atttatacac aggtaagggc ttgttttgaa tgtgtgccgc taaagccttg
     1081 accaaatacg tggcttgttg atgagctgtt tgggcgcgtg gcggcacagg acgttccata
     1141 ttgggcaatg tacaagacgc acaatcacca aaggcaaaaa tattgttgtc acgggtggtt
     1201 tgtaagctcg gtttaaccac taattgatta atacgattgg tttctaaacc tgcaatgttt
     1261 tgtaaaaagt caggagcttt aatgcccgca gaccatactt ttaaatcggc aggaatacgc
     1321 tgagcacctt cacactcaac ataatgtgct tgaatagaaa gcaccttcgt tttagttaac
     1381 acgttaatgc ctttagcttt gagctgtact tcggcttcgg cagagacttg cggagggagt
     1441 acaggcaata ctctatctgc cgcttcaatt aaggtaattt tgacgttttg gggttgaata
     1501 tcatctaggc cataacgagc aaactcatgg ct
//
LOCUS       DAOQMB010000556         1525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65138_length_1525_cov_5.876871, whole genome shotgun sequence.
ACCESSION   DAOQMB010000556 DAOQMB010000000
VERSION     DAOQMB010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65138_length_1525_cov_5.876871"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>1525
                     /gene="dnaB"
                     /locus_tag="PLY05_12020"
     CDS             253..>1525
                     /gene="dnaB"
                     /locus_tag="PLY05_12020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004903904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNC04067.1"
                     /translation="MNTNLQFADDDVVTSLRVPPHSLTLEQAVLAGLMMEPEVWDNLE
                     SVIGEQDFYSPRHRMIFAAIAHLIRLEQPCDALLVAKHMEDTGKLEKAGGEAYLEQIL
                     RDSPATTTNILIYAERIREFSVLRQLITSTNEIANLAYHPKGESASTILDQAETKIFA
                     IAEQQKNRSVKNGPQLIKPLLAQTIQKIDELYHSTEPLTGLSTGFTDLDKRTSGLQGG
                     SLVIVAARPSMGKTTFAMNLVESIIFNTQLPALVFSMEMPSEQLLLRMLSSIGGVDQG
                     RVRSGKLEDEDWPRITSAMTQLSEKLLYIDDSAALPPSELRARARRVARDHGGKMGLI
                     MVDYLQLMRVPGFDAGNRVNEVGEISRSLKALSKELNCPVVALSQLSRNVEQRPNKRP
                     VMSDLRESGAIEQDADLIMFIYRDEVYNPESK"
BASE COUNT          466 a          297 c          296 g          466 t
ORIGIN      
        1 attgcatagc gatgtaatgg cagaagttgc tgttactatc gttgctgagt aatctagtta
       61 ctagactact caaaaaaagc actattgttt taaacaatgg tgctttttta ttttgtcgtg
      121 taactctcgt ttatcacttg tcaggactaa caaaaacact taaaaaagcc tattatcaac
      181 acaagcacac tgccctgtaa aaatcttaaa agtatctgac caactcaact tcaattgatt
      241 acttgattag ctatgaatac caatctgcaa tttgctgacg atgatgttgt cacttcatta
      301 cgagtccctc ctcatagctt aaccctagaa caagcggttt tggcaggttt aatgatggaa
      361 cccgaagtgt gggacaatct tgaatcggta atcggtgagc aagattttta ctcacctcgt
      421 catcgaatga tttttgcagc aattgcccat ttaattcgtt tggaacaacc ctgtgatgcc
      481 ttattagtgg ctaaacacat ggaagataca ggcaaactcg aaaaagcagg tggtgaagct
      541 tatttagaac agattttaag agattcacct gccacgacga ccaatatttt aatttatgcc
      601 gaacggattc gtgaattttc ggtattacgc caattaatta cctcaaccaa tgaaattgct
      661 aatttggctt atcatcccaa aggcgaatcg gcatcgacta ttttagacca agctgaaact
      721 aaaatttttg ccattgccga acaacaaaaa aatcgtagtg tcaaaaatgg ccctcaatta
      781 attaaaccat tattagcaca aaccattcaa aaaattgatg aactatatca ttctaccgaa
      841 cccttaactg gtttaagtac aggttttacc gatttagata aacgcacttc gggtttacaa
      901 ggtggtagtt tggtgattgt tgctgctcgt ccaagtatgg gtaaaactac ttttgccatg
      961 aatttggtcg aaagtattat ttttaatacc caattgcctg ctttagtatt ttcgatggaa
     1021 atgccttcgg aacaattatt attaagaatg ttgtcgtcaa ttggtggcgt agatcaaggc
     1081 cgtgtccgtt caggtaaatt agaagatgaa gattggccgc gtattacttc tgccatgacc
     1141 caactcagtg aaaagctatt atatattgat gatagtgccg cgctaccacc ttccgaatta
     1201 cgggcgcgcg cacgacgtgt ggcacgcgat catggtggca aaatgggctt aatcatggtc
     1261 gactacctac aattaatgag agtacccggt tttgatgcag gcaaccgtgt taatgaagtc
     1321 ggtgaaattt ctcgctcttt aaaggcactc tctaaagaat taaattgccc tgtggttgct
     1381 ttatcacaat taagtcgtaa tgttgaacaa cgtccgaata aacgccctgt catgtctgac
     1441 ttacgtgaat caggtgctat tgaacaagat gctgatttaa ttatgtttat ttatcgagat
     1501 gaggtatata atcccgaatc aaaag
//
LOCUS       DAOQMB010000557         1523 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65316_length_1523_cov_11.942779, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000557 DAOQMB010000000
VERSION     DAOQMB010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65316_length_1523_cov_11.942779"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLY05_12025"
     CDS             complement(<1..634)
                     /locus_tag="PLY05_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004830254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNC04068.1"
                     /translation="MTELSSLSQWLHHLENQHHKAIDMGLARVQAVAQVADLLTLNCP
                     VITVGGTNGKGSTVATLTAIYCAAGYKVGTYTSPHIIDFNERIAINNQPVSDELLIQA
                     FNAVEAARAITNISLTYFEFTTLAAFWIFKQAKLDIAVLEVGLGGRLDAVNIIDADVA
                     VVTSIGLDHTDWLGDTREAISFEKAGIFRAHQAAIYGETNPPQPLVDHAQQ"
     gene            complement(651..>1523)
                     /gene="accD"
                     /locus_tag="PLY05_12030"
     CDS             complement(651..>1523)
                     /gene="accD"
                     /locus_tag="PLY05_12030"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HNC04069.1"
                     /translation="KESFSMSWLGKILPNAGKSTEERKMSVPEGLWKKCPKCDAVLYQ
                     AEVERNLHVCPKCDHHLRIGARQRIDMFLDPEGREEIGMTLQPEDRLNFKDSKRYVDR
                     LSDAQKETNETDALIVVKGTINEMPVVVAAFEFSFMGGSMGAVVGARFIKAAEMCLRE
                     RIPLVCFATSGGARMQEALFSLMQMARTSAIIEQMKLQGIPYISVLVDPVFGGVSASL
                     AMLGDINVAEPYAVIGFAGPRVIEQTVRQVLPEGFQRSEFLLAHGMVDMIVHRHEMRD
                     TLYRVLAKLTRRPL"
BASE COUNT          474 a          359 c          292 g          398 t
ORIGIN      
        1 tttgttgagc atgatcgact aagggttgag ggggatttgt ttcgccataa atagccgctt
       61 gatgggcgcg aaaaatccct gctttttcaa agctaatggc ttcgcgggta tcacccaacc
      121 aatcggtatg atctaaacca atactggtga cgaccgcaac atcggcatca ataatattaa
      181 ccgcatctaa acgaccaccc aaccccactt ctaaaacagc aatatctaat tttgcttgtt
      241 taaaaatcca aaaagccgct aaagtagtaa attcaaaata agttaaagaa atattggtga
      301 tggcgcgagc agcttctacg gcattaaagg cttgaatcaa taactcatca ctaacaggct
      361 gattattaat ggcaatacgc tcattaaaat caataatatg tggtgaagta tacgtcccta
      421 ctttatagcc tgccgcacaa taaattgcgg ttaaggtggc aaccgttgag cctttgccat
      481 ttgtgccacc cacagtaata acagggcaat ttagcgttaa taaatcagcc acttgggcaa
      541 ccgcttgcac acgtgccaag cccatatcaa tagctttatg atgctgattt tcgagatgat
      601 gcagccattg gctaagcgaa gatagctcgg tcatagttta aatagagtaa ttacaaagga
      661 cggcgggtta atttggctaa aacacggtat aaagtatcgc gcatttcgtg gcgatgaaca
      721 atcatatcaa ccataccatg tgctaataaa aattctgagc gttgaaaacc ttcgggtaag
      781 acttggcgaa ccgtttgttc aataacacga gggcctgcaa agccaatcac cgcataaggt
      841 tcggcaacat taatatcacc caacatcgct aaactggctg atacaccacc aaacacagga
      901 tcaaccaata ccgaaatata aggaatacct tgtaacttca tttgctcaat aattgcagat
      961 gtacgcgcca tttgcattaa tgaaaacaag gcttcctgca tgcgcgcccc acctgatgtc
     1021 gcaaaacaca ctaaaggaat acgctcacgc aaacacattt ctgcggcttt aataaatcgt
     1081 gcgcccacca ctgcccccat tgagccgccc atgaaggaaa actcaaaggc ggcaacgaca
     1141 acaggcatct cattaattgt gcctttgacc acaattaaag catctgtttc gttggtttct
     1201 ttttgggcat cgcttaaacg gtcaacataa cgtttgctat ctttaaaatt aagacgatct
     1261 tcgggctgca aagtcatgcc tatttcttca cggccttcgg ggtctaaaaa catatcaatc
     1321 cgttgacgcg caccaatacg caaatgatga tcacacttag gacaaacgtg taaattgcgc
     1381 tcaacttcgg cttggtaaag caccgcatcg catttgggac attttttcca taaaccttca
     1441 ggaacagaca ttttgcgttc ttcggtactt ttacctgcat tgggcaaaat ttttcctaac
     1501 cagctcatag aaaaactctc ttt
//
LOCUS       DAOQMB010000558         1522 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65404_length_1522_cov_13.360600, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000558 DAOQMB010000000
VERSION     DAOQMB010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65404_length_1522_cov_13.360600"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="PLY05_12035"
     CDS             <1..1464
                     /locus_tag="PLY05_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HNC04070.1"
                     /translation="YAFAFIIIGLLLLLRCKQDWLAIVKSVVVFLAIALICACASFIS
                     YQLNLLLAARILQEISVILTGILTIRVIGLSIFRSILPLLHVHPPRILEDIILVLAYI
                     AWGMIRLSTAGVNLSGLVTTSAVITGIIAFSMQDTLGNILGGLALQLDNSISIGSWIK
                     VDDVSGQVIEVHWRHTAVRTRNGEIVVIPNSMLMKAKVIIVGGEDVPQWRRWVYFAVS
                     NTIPPQRVINSIDKALNQAEIPLVSHHPKPQCVLMDYKDGLCVYAVRYWLQNAQIDDP
                     TDSTIRVHIFVALQRQGYALASPNFAINMTTESLEREAHLHNTELAIREHKLGKMDLF
                     STLSHEERQHLAKTLTYAPFAKGDVITRQGDVAHWLYVLVSGEVDIWYEVNTQERKLL
                     TTLPAGRVFGEMGLMTGEPRRATVIAKTDVECYRIDKPSFEHIIQSRPELANEFAHIL
                     AERNQQLVAVQQNGNHNNHAQHHAKILDGIRRFFRLG"
BASE COUNT          444 a          296 c          306 g          476 t
ORIGIN      
        1 tatgcttttg cttttatcat cataggctta ttactgttat tgcgttgcaa acaggattgg
       61 ttagctattg ttaaaagcgt cgtggtattt ttagcgattg cactgatttg tgcatgtgca
      121 agttttatta gttatcaatt aaaccttcta ttagctgcac gtattttgca agaaatatcc
      181 gttattttga cgggcatact caccattaga gtcatcggac tgagtatttt tcgttctatc
      241 ttacctctat tacacgttca tccaccgcgt attttagaag atattatttt agtgcttgcc
      301 tatattgcat ggggcatgat tcgtttaagt acagcaggag ttaacttatc aggtttagtg
      361 acaacctcag cagtcattac tggtattatc gctttttcga tgcaagatac cctaggcaat
      421 attttagggg gattagcctt acagttagat aactccatca gcattggcag ttggattaaa
      481 gtggatgatg tcagtggcca agtaatcgaa gttcattggc gacatacggc agttcgtact
      541 cgtaacggcg aaatagtcgt tattcccaac agtatgttaa tgaaagctaa agtgattatt
      601 gtcggtggtg aagacgttcc ccaatggcga cgttgggtat attttgcggt gagtaatact
      661 attccccccc aacgggtaat taacagcatt gataaagccc ttaatcaagc cgaaatacct
      721 ttagttagtc atcatcccaa accccagtgc gtattaatgg actataaaga tggtctatgt
      781 gtttatgcgg tacgctattg gctacaaaac gctcaaattg atgatcccac cgatagcact
      841 attagagtac atatttttgt tgccttacaa cgtcaaggtt atgcccttgc ctcgcctaac
      901 tttgccatta atatgactac tgaatcatta gaacgtgaag cacatttaca caataccgaa
      961 ttagctatcc gtgaacacaa acttggcaaa atggatctat tttcaacttt atcacatgaa
     1021 gaacgccaac accttgccaa aaccttgact tatgcaccat ttgctaaagg tgatgtgatt
     1081 actcgccaag gtgatgtggc gcattggtta tatgtattag tcagtggtga agtggatatt
     1141 tggtatgaag ttaacacgca agaacgtaaa ttattaacca ccctccctgc tggacgagtt
     1201 tttggtgaaa tgggcttaat gacgggagaa cctcgccgag ccactgtcat cgccaaaact
     1261 gatgtggagt gttatcgcat agataaacca agttttgagc atattataca atcgcggcct
     1321 gaactagcta atgagtttgc tcatattctt gccgaacgta atcaacagtt ggttgcggta
     1381 caacaaaatg gcaaccacaa caaccatgct caacatcatg ccaaaatttt agatggaatt
     1441 cgtcgctttt ttagattggg gtaattggca tttaacaaaa aatcaactat cttcaaaaga
     1501 gaataattaa ttaaagatca tc
//
LOCUS       DAOQMB010000559         1520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65632_length_1520_cov_2.074403, whole genome shotgun sequence.
ACCESSION   DAOQMB010000559 DAOQMB010000000
VERSION     DAOQMB010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65632_length_1520_cov_2.074403"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="PLY05_12040"
     CDS             complement(<1..355)
                     /locus_tag="PLY05_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Cas7f/Csy3"
                     /protein_id="HNC04071.1"
                     /translation="MATKTSTKIKTASVLSFNRHLDPSDGRMTAVNWTDIQQGAGSTA
                     IKIEEKSVRGTISNRPKNSKEVDPAYINAAVEKANLQTVDIAALPQGTDTVAVTFTLR
                     VLPNVGEPSACNDESF"
     gene            complement(358..1461)
                     /gene="csy2"
                     /locus_tag="PLY05_12045"
     CDS             complement(358..1461)
                     /gene="csy2"
                     /locus_tag="PLY05_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HNC04072.1"
                     /translation="MSYSYPKALYLVRLQVENVSIISSHLTWGFPAPSAFTGFSHALQ
                     RKLNQLDGYHQIDCCGVGIICHQFSPQITKSSDYRYAPYQLNLARHPLEADGSTPAMV
                     EEGKGHMEVSLIIGLSGDDLEQHLSANDDDLNVQSQQLIKHLNHLVYSMRLAGGAVFP
                     HQLIKAKLVNWSLAEAAKKTKTLCRYLLPSFALIHRHDVLIAHQTWLSQYSSYLETQG
                     HAPTVLDTLLDISRLNLNSVSSQESKANGEQQGDWQVRPRPKHLQGWLVPIPIGYAAL
                     IELQDKGTITGLRDNQYPATFVETLLSLGEWQSPHRIEDLTRTLWTYNPSPKDGVYEL
                     LQPFAQKQNTQTTFDDFEEESFDYYPNSEDEAI"
BASE COUNT          442 a          295 c          314 g          469 t
ORIGIN      
        1 caaaactttc atcattacag gcagacggtt cacccacatt cggtaataca cgtaaggtga
       61 aagtcactgc aacggtatcc gtgccttgag gcagtgcagc aatatctacc gtttgtaaat
      121 tggctttttc tacagccgca ttaatataag cagggtcaac ttctttactg ttttttggac
      181 gattagaaat tgtcccacgt accgattttt cctcaatttt aatagctgtt gagccagcac
      241 cttgttgaat atcagtccaa tttaccgcag tcatacgccc atcagaaggg tctaaatgac
      301 ggttaaagga taatactgat gcggttttga tttttgttga tgttttagta gccatgatta
      361 aattgcctcg tcttctgagt ttggataata gtcaaatgat tcttcttcaa aatcatcgaa
      421 tgtcgtttga gtgttttgtt tttgtgcaaa gggttggagg agttcatata cgccatcttt
      481 aggggagggg ttatatgtcc aaagagtgcg agttaagtct tcaatgcgat gaggactttg
      541 ccattcgcct aaactcaata gagtctcaac aaaagtggct ggatattgat tgtcacgtaa
      601 accagtgatt gtgcctttgt cttgaagctc tattaaagcc gcataaccaa tcggaatagg
      661 tacaagccag ccttgtaaat gcttagggcg aggacgaact tgccaatcac cttgttgctc
      721 accattggct tttgattctt ggcttgacac actgtttaaa ttgagacggc taatatcaag
      781 caaagtgtct aatacagtag gcgcgtgtcc ttgtgtttct aagtacgatg agtattggct
      841 taaccatgtt tgatgtgcga ttaacacgtc atgtcgatga attaatgcaa aacttggcaa
      901 taaataacga cacaaagttt tagtcttttt agcggcctca gctaatgacc aattaaccag
      961 tttagctttg ataagttgat ggggaaaaac agcaccacct gccaagcgca tactataaac
     1021 aagatgattt aagtgtttaa taagttgttg gctttgtaca tttaagtcat catcattagc
     1081 agataaatgc tgctctaaat cgtcaccact taaaccaata attaagctga cttccatgtg
     1141 gcctttgcct tcttcgacca ttgctggcgt agaaccatcg gcctctaaag gatgtcgtgc
     1201 taaattgagt tgataagggg cataacgata gtcgctactt ttagtgattt gagggctaaa
     1261 ttgatgacaa ataatgccaa caccacaaca atcaatctga tgatagccat ctaattgatt
     1321 taatttacgt tgtaaagcat ggctaaagcc cgtaaatgca ctgggtgcag gaaagcccca
     1381 cgttaaatga ctactaataa tcgaaacgtt ttcgacttgc aaacgtacaa gatatagtgc
     1441 ttttggatag gagtaactca tttatgcaaa ctcctcctta agtagttgta atttatcgcg
     1501 taatgccagt gaccatacac
//
LOCUS       DAOQMB010000560         1519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65648_length_1519_cov_12.676913, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000560 DAOQMB010000000
VERSION     DAOQMB010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65648_length_1519_cov_12.676913"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1519
                     /locus_tag="PLY05_12050"
     CDS             <1..>1519
                     /locus_tag="PLY05_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC04073.1"
                     /translation="ESKLMGISGKLSRFFLRSQLTPLLAVIAFLLGLFALAVTPREEE
                     PQIDVTMANVMIPFMGAKASDVERLVAVPAEQVLSQIQGIEHVYSVSKPNMALLTVQF
                     KVGVARNEAIVRLYEAINNNKDWLNPQLGVGEPLIKTKGIDDVPILSLTLWQAQGNKG
                     AYDLGRIAHSIELELKRVSGTREVYSIGTSPRVLNIELDSARLQAHQLSALEVSQRLQ
                     QSNSSLSSGDLVANNRVTHIEANDFLRSAEDVGRLVIAVNDGQAIYLNDVATIHDGAS
                     TASQYSWLGTGMAAAQRGISQQGEFPSVTLAISKKTGENAVDVAERVLQRVNSLKNTM
                     IPADVEVTVTRNYGETANEKAQKLMQKLLFATLSVIVLVFIALGKREAVIVGLAVLLT
                     LAATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRRMQLDNKPLAEVIPA
                     AVDEVGNPTILATFTVIAALLPMAFVSGLMGPYMSPIPINASMGMLISLFIAFSIT"
BASE COUNT          395 a          308 c          365 g          451 t
ORIGIN      
        1 aggagtctaa actcatgggt atttcaggta agttaagtcg cttttttttg cgctcacaac
       61 tcacacccct gttggcggtg attgcctttt tgttgggttt gtttgcctta gccgttacgc
      121 ctcgtgaaga agaaccacaa attgatgtga cgatggctaa tgtgatgatt ccatttatgg
      181 gtgcaaaagc cagcgatgtt gaacgtttag tggctgtgcc tgccgagcaa gtgttgtcac
      241 aaattcaagg cattgaacac gtttattcgg tctctaaacc gaatatggcc ttgttaaccg
      301 tacaatttaa agtcggtgtc gcgcgcaacg aagccattgt gcgtttatat gaagcgatta
      361 ataacaacaa agattggtta aacccacaat taggcgttgg cgaaccctta attaaaacca
      421 aaggcattga tgatgtgcca attttgtcgt taaccttatg gcaagcgcaa ggcaacaaag
      481 gtgcttatga tttaggtcgt attgcccata gcatcgaatt agagctaaaa cgggtttcag
      541 gcactcgcga agtatatagc attggcacat cccccagagt attaaacatt gagttagata
      601 gcgcacgttt acaagcgcat cagctcagtg ctttagaagt cagtcaacgt ttacaacaaa
      661 gtaatagctc cttaagctct ggcgacttag tggccaataa ccgtgtgacc catattgaag
      721 cgaatgattt tttgcgttct gctgaagatg ttggtcgttt ggtaattgcc gttaatgatg
      781 gccaagcgat ttatttaaat gatgtcgcca ccattcacga tggtgcaagc accgccagcc
      841 aatacagttg gttggggaca ggcatggctg ccgcacaacg aggcattagc caacaaggtg
      901 aatttccttc tgttacttta gcaattagca aaaaaacggg cgaaaacgcg gtagatgtgg
      961 cggagcgcgt gttacaacgg gtgaatagcc taaaaaacac aatgattcct gccgatgttg
     1021 aagtgacggt cacacgcaac tatggcgaaa cagccaacga aaaagcgcaa aaactgatgc
     1081 aaaaattatt gtttgccacc ttatcagtca ttgtgttggt gtttattgcg cttggcaaac
     1141 gtgaagcggt gattgtcggt ttagcggtgt tattaaccct tgccgcgact ttatttgctt
     1201 cgtgggcatg gggctttacc ctcaatcgtg tgtctttatt tgccttaatt ttttcgattg
     1261 gtattttggt cgatgatgcc attgtggtgg ttgaaaacat tcaccgtcgg atgcaattag
     1321 acaataagcc tttagctgaa gtgattcctg cggcggttga tgaagttggt aacccaacta
     1381 ttttagccac gtttacggtg attgctgcgt tattaccgat ggcctttgtc agtggtttaa
     1441 tgggccctta tatgagccct attccgatta atgcctcgat gggcatgctc atttctttgt
     1501 ttattgcctt tagcattac
//
LOCUS       DAOQMB010000561         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65830_length_1517_cov_34.091655, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000561 DAOQMB010000000
VERSION     DAOQMB010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65830_length_1517_cov_34.091655"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="PLY05_12055"
     CDS             complement(<1..>1517)
                     /locus_tag="PLY05_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HNC04074.1"
                     /translation="IVLPDYQKKPFEPKQNHTTQSTDEEAIETHRNAPRGNDAMLYGI
                     FGDFGRIAAKNTEVNPYAAAFNSMIYLSSAIGRDVFFYVGNDIHHVRLFGLHIGRSSK
                     GQKGGAHSLLNRVEKHLAEREPTLAPRKHTGGLSSREGLAALLHDGYMDGKQEVEPIT
                     DKRLFIRESEFANVMHQASRNGNTLSAALRDGWDGIGSQPATKHRGIWSTNPHIAIAA
                     ACTPVELITLTTKNDVSNGFLNRFVIFWAERTKLVPFPESTPQHTVDDLAQRLANIID
                     FAKGDYHWDTNPSLSVENTRQITLSDEAKKVYGHIYIKVFPKLNDGEKVTALTERKAP
                     YLLRIAALFALSDFSLVIEAHHINAAYEWIKYWHESVKFIFNDEAEEVQNTQIAINAE
                     KILDYLKSKGSKHKATQTELSENVFNKHASSKEINAAISSLLSTTPPQIEVLTGERAD
                     GSNGRKPKLYALFKNSQNPCEISEISEITTAATKKPKFAKCEISEISEISPLNSQ"
BASE COUNT          373 a          301 c          318 g          525 t
ORIGIN      
        1 ttgcgaattt agggggctaa tttcgctaat ttcgctaatt tcgcattttg cgaatttagg
       61 tttttttgtt gcggctgtag ttatttcgct aatttcgcta atttcgcaag ggttttgcga
      121 atttttaaaa agtgcgtaaa gttttggttt tcgtccatta ctaccatccg cacgctctcc
      181 agttaagact tcaatctgtg gcggagtggt gcttaataag ctgctaatgg ctgcattaat
      241 ctctttacta cttgcgtgct tgttaaagac gttttctgat agctcggttt gggttgcttt
      301 gtgtttacta cccttagact ttaagtaatc aagtatcttt tcggcattga ttgctatttg
      361 tgtgttttgc acttcttcgg cttcatcatt aaaaataaat tttactgatt cgtgccagta
      421 tttaatccac tcataggctg cgttaatgtg gtgtgcttca atcactaagc taaaatcgga
      481 taatgcaaat aaggcggcta tacgcaacaa ataaggggct ttacgttcgg ttaatgcggt
      541 gactttttca ccatcattca atttaggaaa tacttttata taaatatgac catacacttt
      601 tttagcttca tcactcagcg taatttgcct tgtgttttca acggatagac tggggttggt
      661 gtcccaatgg taatcacctt ttgcaaaatc aataatgttt gctaatcgct gtgctaaatc
      721 atcaacggta tgttgtggcg tactttcagg aaatggcact aactttgtcc gttctgccca
      781 aaaaataaca aaacgattca aaaagccatt agatacatcg tttttggtgg ttaaggtgat
      841 taactcaact ggggtacaag cggctgcaat cgctatatgt gggtttgttg accaaatccc
      901 cctatgcttg gttgcaggtt gtgagcctat cccatcccat ccatctctta acgcggctga
      961 aagtgtgttg ccgtttctac ttgcttggtg catgacgttg gcaaactccg attcacggat
     1021 aaaaagacgt ttatcggtaa tgggttcaac ttcttgtttg ccgtccatat aaccatcatg
     1081 caataacgcg gctaaacctt cacgcgaaga taatccgcct gtgtgctttc ttggtgctag
     1141 tgttggctca cgttctgcta ggtgtttttc cactcggtta agtaatgagt gtgcgcctcc
     1201 cttttgccct ttgcttgatc tgccaatatg caaaccaaaa agacgtacat ggtgtatatc
     1261 attgccaaca taaaaaaata catctctacc aattgcagac gacaaataga tcattgagtt
     1321 aaaagcggct gcataaggat taacttcggt atttttagcg gctatacgtc caaagtctcc
     1381 aaaaataccg tatagcatgg catcgttgcc gcgtggtgcg tttcggtggg tttctattgc
     1441 ttcttcgtct gtgctttgtg ttgtatggtt ttgctttggt tcaaagggct ttttttgata
     1501 gtcaggtaaa acaatac
//
LOCUS       DAOQMB010000562         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_65838_length_1517_cov_6.954856, whole genome shotgun sequence.
ACCESSION   DAOQMB010000562 DAOQMB010000000
VERSION     DAOQMB010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65838_length_1517_cov_6.954856"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..>1517
                     /locus_tag="PLY05_12060"
     CDS             255..>1517
                     /locus_tag="PLY05_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HNC04075.1"
                     /translation="MINSIAQKTVAVVGLGYVGLPLALAFGKKIKTIGFDISQAKLMA
                     YRQGIDPTGEMESSAFAQAQHLIFTHDNQQLAQADYIVVAVPTPIDNAKQPDLTPVVK
                     ATEALAPYIKDGAIIIYESTVYPGVTEDVCSPIIEKLSGKQRGIGFKLGYSPERINPG
                     DKVHRLETIIKIVAGEDQDSLDQVAALYELIVEAGVYRASSIRVAEAAKVIENTQRDV
                     NIALMNELAVIFNLMGIDTLEVLQAAGTKWNFLPFRPGLVGGHCIGVDPYYLTHKVST
                     LGYTPEVILAGRRINDRMGTYIAQQTIKQMILLGTMTKGATVLILGLTFKENCADIRN
                     TKVIDVISELKDYGLNVVVWDPVADAHEAHEEYGMDLLPNLDQVSHVAAVVAAVAHQQ
                     ILEMDLQHLQAKTVANPTPWIDVKSIFDQ"
BASE COUNT          456 a          264 c          316 g          481 t
ORIGIN      
        1 aaaaagatgg ttctttaacc ctagaagatt tagctgaaca attattatta atggctttag
       61 ggcaagtcgc ttagatttgt cattttgtcc gacactgcat taactttagg ccgataatat
      121 ggtatatagt cggccgcaat catttgtgtc taaactttga gtttttgagt gcgttcttat
      181 caatcacctt agatttatat ccatcagttt tgttaaatac taagctgttc ccagagaaag
      241 gacattcgct atttatgatt aattctattg cgcaaaaaac agttgctgtt gttggtttgg
      301 gttatgttgg tttaccgctg gcattggcct ttggaaaaaa aataaaaaca attggttttg
      361 atattagcca agccaaattg atggcatatc gccaaggcat tgatccgaca ggcgaaatgg
      421 aatcttctgc atttgctcaa gcccaacatt taatctttac gcatgataat caacaattag
      481 cacaagctga ctatattgtg gttgcagtcc caaccccgat tgataatgcc aaacaacctg
      541 atttaacacc agtggttaaa gccacagaag ccttagcacc ctatattaaa gatggcgcga
      601 ttattattta tgaatcaacg gtttatcctg gtgtgaccga agatgtttgc tcaccgatta
      661 ttgaaaaatt atcaggcaaa caacgtggta ttggctttaa attaggttat tcacctgagc
      721 gtattaatcc tggtgataaa gttcatcgtt tagaaactat tattaaaatt gttgctggcg
      781 aagatcaaga ctcattggat caagtcgctg ctttgtatga gttaattgtt gaagcgggtg
      841 tgtatcgtgc ttcgagtatt cgtgttgcag aagccgccaa agtgattgaa aacacccaac
      901 gcgatgtcaa tattgcgtta atgaatgagt tggcagtgat ttttaacctg atgggtattg
      961 atactttaga agtattacaa gccgcaggca caaaatggaa tttcttacct ttcagaccgg
     1021 gtttagtcgg tggtcattgt ataggtgtag atccatatta tttaacccac aaagtcagca
     1081 cattaggtta taccccagaa gttatcttag ctggtcgtcg aattaatgat cgcatgggca
     1141 catatattgc acagcaaacc attaaacaaa tgattttgct gggtacgatg actaaagggg
     1201 caaccgtatt gattttaggt ctgacattta aagaaaactg tgcggatatt cgtaatacca
     1261 aggtaattga tgtcattagc gaattaaaag actatggctt aaatgtggtt gtttgggatc
     1321 ctgtggccga tgcccatgaa gctcatgaag aatatggtat ggatttatta cccaatttag
     1381 atcaagtcag tcatgtagct gctgttgttg ccgcagttgc acatcaacaa attttagaga
     1441 tggatttaca acatttacaa gctaaaaccg ttgcaaatcc aacgccgtgg attgatgtaa
     1501 aatctatttt tgatcaa
//
LOCUS       DAOQMB010000563         1512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_66270_length_1512_cov_13.144818, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000563 DAOQMB010000000
VERSION     DAOQMB010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66270_length_1512_cov_13.144818"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(124..849)
                     /locus_tag="PLY05_12065"
     CDS             complement(124..849)
                     /locus_tag="PLY05_12065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017394891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HNC04076.1"
                     /translation="MITQLDIHSVCVQLQRKAIKNLYIRVLPPDGQVNISAPLRMSET
                     TIRSVVIERLPWIKKQQDKFSQAIKQKNVSTNTDELIYLWGKTYRLEFIEHIGRHKEQ
                     VFDDNLCLYIKPNSTIQNKALVLEAFYRAELKNELEKMLVDWQKIMGVTASSFGIKRM
                     KTRWGSCNTGTKKIWFNLELAKKPMQCVEYVLVHELAHLLEPSHNHRFKAYMSQFLPD
                     WQERKALLNAIPMFNEVCFDEES"
     gene            complement(1090..1302)
                     /locus_tag="PLY05_12070"
     CDS             complement(1090..1302)
                     /locus_tag="PLY05_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04077.1"
                     /translation="MDGENKQRLRGLMRNANLDTLAVKKLLEQQTKRKYSIRTVQAWA
                     ADSSKASSRECPEWVLENLEQIIKEL"
     gene            complement(1307..>1512)
                     /locus_tag="PLY05_12075"
     CDS             complement(1307..>1512)
                     /locus_tag="PLY05_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04078.1"
                     /translation="LVLQQNKGKIEHAQVEKKTIKADSIQLLQQGKKLIVAVKSRKAS
                     YDIELPIAAQQLQELLKQLLKES"
BASE COUNT          426 a          290 c          241 g          555 t
ORIGIN      
        1 aaaagactat ccataaaaag ataacctatt agctactatt ttattattat agcaacttta
       61 gacaaaatat tgtctatttt ttattttact attgaacata gtgtaataaa aaattgagcg
      121 tgtttaactc tcttcatcaa agcagacttc attaaacatc ggtatagcat ttaataaagc
      181 cttccgctct tgccagtcgg gtaaaaactg gctcatataa gccttaaaac gatgattatg
      241 actaggctct aacaaatggg ctaattcatg caccaataca tattcgacgc attgcatggg
      301 ctttttcgct aattcaagat taaaccaaat ttttttagtg cctgtattac aactacccca
      361 acgggttttc atgcgtttaa tgccaaaact agatgctgtg acacccatga tcttttgcca
      421 gtcaacaagc attttttcta attcattttt taattcagct cgataaaacg cctctaaaac
      481 taatgcttta ttttgtatgg tgctatttgg cttaatgtat aaacatagat tatcatcaaa
      541 gacttgctcc ttatgtcggc caatatgctc tataaactct aggcgatatg ttttccccca
      601 cagataaatg agttcatcgg tgtttgttga gacatttttt tgcttaatgg cttggctaaa
      661 tttatcttgt tgttttttaa tccacggtaa acgctcaatc actacgctac gaatagttgt
      721 ttcgctcata cgtaatggtg cgctaatatt tacctgtcca tcgggcggta atacacgaat
      781 atataagttt ttgatggctt tgcgttgtaa ttgtacgcaa acgctatgta tgtctaattg
      841 agttatcatt ttataaatta tcttagttac acttaaaaaa tgctgacatt acacggggat
      901 tatttgtact agctccgaat taatttctca tcgcagggcg tcgattggca tatttgaatt
      961 attaatcttt actcttttac tctcacttaa aattgactca ataataatac aatactcagc
     1021 taattctact ttaatttcag tattaatcgg cagagtaatt tctaaaaatt gagcatgctc
     1081 aattgttgct taaagctctt taatgatttg ttctaagttc tctagcaccc attctggaca
     1141 ctctctagaa cttgcttttg acgagtcagc agcccacgct tgcacggttc taatactgta
     1201 tttacgttta gtttgctgtt ctagcagttt tttaacggcc aaagtatcta aattagcgtt
     1261 acgcattaaa ccgcgaagtc tttgtttgtt ttcaccatcc atcaacttag ctttccttga
     1321 gcagttgttt taataactct tgtaattgtt gtgctgctat tggcagctct atgtcatagc
     1381 tagccttacg cgacttgacg gcaacaatca attttttgcc ttgttgtagc aattgaatgc
     1441 tgtcagcttt gatagtcttt ttctcgactt gagcatgctc aattttgcct ttgttttgtt
     1501 gcaaaaccag at
//
LOCUS       DAOQMB010000564         1509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_66531_length_1509_cov_8.356259, whole genome shotgun sequence.
ACCESSION   DAOQMB010000564 DAOQMB010000000
VERSION     DAOQMB010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66531_length_1509_cov_8.356259"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="PLY05_12080"
     CDS             <1..1005
                     /locus_tag="PLY05_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC04079.1"
                     /translation="CVLSLLPMVVYANPPTKKPPPTVEVQAVGQQILGQQLTALAVLK
                     AQESIIIKPEVAGRVTAINFNEGQIVKENQLLIQLDDDLLKAELATYQASLDLAQTEY
                     QRYQALLKEQQVAVLDYERKKAELAQIKASMALVQAKIRQKQIRAPFTGVIGLRQFSI
                     GDVLQANQNLVRLNKMNALKAEIKIPETNSQLISLKQSVNLQVDGLANFAVKGYVAAI
                     EPSLDANTRALIVRVNIPQADSRVRDGMTARASFVLGQQQAIVVPEQALVAQGGKFVV
                     FVFKNDAVTATPVEVGQRQIGQVVIKKGLQVGDMVVISGQNKLPKPQMPVKAITVQGV
                     "
     gene            1008..>1509
                     /locus_tag="PLY05_12085"
     CDS             1008..>1509
                     /locus_tag="PLY05_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC04080.1"
                     /translation="MQLPTLCIRRPVLAIVLNLLIVLAGLLAFDRLTVREYPNIDIPT
                     VTVDTNYTGASAPIVEATVTKILEDSLSGIEGIDYLSSISRTGRSQITIAFKQGREID
                     SATSDVRDRVSRVRGRLPDTIDEPVITKTEADAQPIIWLAFSSQQHTPLQINQFADKQ
                     VKSRLQT"
BASE COUNT          464 a          282 c          332 g          431 t
ORIGIN      
        1 tgtgttctta gtttactgcc aatggtagtt tacgccaatc cacctaccaa aaaaccgcct
       61 cccaccgttg aagtacaagc tgttggtcaa caaatattag gtcagcaatt aaccgcttta
      121 gcagttttaa aagcgcaaga gtctattatc attaaacctg aagtggcggg tagggtgacg
      181 gcaattaact ttaatgaagg tcaaattgtt aaagaaaatc aattattaat tcaattagat
      241 gatgatttat taaaagcgga attagccact tatcaagcca gcttagattt ggcacaaacg
      301 gaatatcaac gttatcaagc gttattaaaa gaacaacaag tggctgtttt agactacgag
      361 cgcaaaaaag ccgaattagc gcaaattaaa gcaagtatgg ctttggtaca agctaaaatt
      421 cggcaaaagc aaattcgtgc gccatttaca ggtgtcattg gtttacgtca gtttagcatt
      481 ggcgatgtgc tacaagccaa tcagaattta gtgcgtttaa ataaaatgaa tgcgctcaaa
      541 gcagaaatta aaattccaga aaccaatagc cagttaattt cattaaaaca aagcgttaat
      601 ttacaagttg atggtttagc gaattttgct gtaaaaggct acgtggcagc aattgagcct
      661 agtttggatg caaatacccg agccttgatt gttagagtca atattcctca agcagatagt
      721 cgagtacgtg atggcatgac cgcacgcgcc agttttgtct tgggacaaca gcaagcgatt
      781 gttgttcctg aacaagcctt ggtcgctcaa ggtggtaagt ttgtggtatt tgtctttaaa
      841 aatgatgcag tgacggccac tcctgttgag gttggacaac gtcaaattgg tcaagtagtg
      901 attaagaaag ggctacaagt aggggatatg gtggtgattt cggggcaaaa taaattgccc
      961 aaaccacaaa tgcctgttaa agcgattact gtacaaggag tttagccatg caactcccta
     1021 ctttatgtat tcgtcgtcct gtattagcaa ttgtacttaa cttgttgatt gtgttggcag
     1081 gactattagc ctttgatcgt ttaacggtgc gtgagtatcc taatattgat attccgacgg
     1141 tgacggttga taccaattat acaggtgcaa gtgcgccgat tgtggaagcg actgttacca
     1201 aaattttaga agattcatta tcgggcatcg aaggtattga ttatttatcg tcaattagcc
     1261 gaacgggtcg aagtcaaatt accatcgctt ttaagcaagg tcgagaaatt gatagtgcga
     1321 cctctgatgt gcgtgatcgt gtgtctcggg tacgaggccg tttgcctgat accattgatg
     1381 aaccagttat caccaaaact gaagccgatg cgcaaccgat tatttggctg gctttttctt
     1441 cacaacaaca tactccctta caaatcaatc aatttgccga taaacaagtt aaaagccgtt
     1501 tacaaacgc
//
LOCUS       DAOQMB010000565         1507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_66705_length_1507_cov_12.696281, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000565 DAOQMB010000000
VERSION     DAOQMB010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66705_length_1507_cov_12.696281"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1507
                     /locus_tag="PLY05_12090"
     CDS             <1..>1507
                     /locus_tag="PLY05_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002116854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HNC04081.1"
                     /translation="LVKHVTPELKAYRTFETAFFGLFHFGTENRSVILILMVAIAATT
                     TTLGLHHIALRPPVTKIDFKIYSIAMVAANVLLLVSSISYWKLQTASGIPIEHPIINY
                     LWVGLFSVLIGISGKQLIKPPADAPEGGSIGMALLSIPLYAFMALNAGLSFIFAGHYS
                     GLAIYLGQMMELSGVFLNLSLYIWVGMLLKQTRVVDLFLNIVRPWKFSPEVLTYILLV
                     AAAVPTAYTGASGIFIIAAGALIYKEVFASGARRQYALAATAMSGSLGVVLSPCLLIV
                     VIAALNKEVTTSLLYGWGLKVFLLTSTMFLIVSLLISKEGFKLAKPAVALPESMRALV
                     PVSAYIVITIIVVMVYRFLLDTKLDEFTAPMILPFIMLAIVWFDKIRRNPEDHAAPEV
                     KERRVGFEESVRVATNDTIGHIGALIMLMALSVSVGGVIERSGMMDAVPETFGSIWVA
                     VSILLVLLVFVGMIMDPFGAVILVSATVAPIAYKNGIDPVHFWMIVLTAFE"
BASE COUNT          349 a          265 c          340 g          553 t
ORIGIN      
        1 gtttagtcaa gcacgtcacg cctgaattaa aagcctatcg cacctttgaa accgcctttt
       61 ttggtttgtt ccattttggt acagaaaatc gctcggtgat tttgattttg atggtagcga
      121 ttgcagcaac cacaacgaca ttgggtttgc atcatattgc gttgcgtcct ccagtcacca
      181 aaattgactt taagatttat tcgatagcaa tggtggcagc taatgtgttg ttgcttgtgt
      241 cgagtatttc ttattggaaa ttacaaaccg catcgggtat tcccattgag catccgatta
      301 ttaactatct gtgggtaggc ttattttctg ttttaattgg catcagtggc aagcagttaa
      361 ttaagccgcc agcagatgca ccagaaggcg gtagcattgg catggcatta ttgtctattc
      421 ctttatatgc gtttatggca cttaatgctg gtttaagttt tatctttgca ggtcactatt
      481 ctggtttggc gatttattta ggccaaatga tggaattatc aggcgtattc ttaaacttgt
      541 cactgtatat ttgggtgggt atgttgctca aacaaacccg tgtagtggac ttgtttttaa
      601 atatcgtgcg tccgtggaag ttttcaccag aagtgttaac ctacatttta ttagttgctg
      661 ctgctgtgcc aaccgcttat acaggggctt caggtatttt tattattgcc gcaggtgcat
      721 taatttataa agaagtgttt gcttctggcg cgcgccgtca atatgcctta gcagccaccg
      781 cgatgtcggg ttctttaggt gtggtattaa gtccttgttt gttaattgtg gtgattgccg
      841 cgcttaacaa agaagtcact acttcgttat tatatggttg gggtttaaaa gtctttttat
      901 taacctctac catgttttta attgtgtctt tattaatttc taaagaaggc tttaagttag
      961 ctaaaccagc cgtagcttta cctgaatcaa tgcgtgcttt agtacctgtt tctgcttata
     1021 ttgtcattac gattattgta gtcatggttt atcgcttctt attagatact aagctagatg
     1081 aatttacagc ccccatgatt ttgcccttta ttatgttggc aattgtctgg tttgataaaa
     1141 ttcgtcgcaa tccagaagat catgccgctc ctgaagtaaa agaacgccgt gttggttttg
     1201 aagaaagtgt tcgtgttgct accaatgaca ccattggtca tattggtgca ttaattatgc
     1261 ttatggcact ctcggtgagt gtgggtggtg tgattgaacg ttcaggtatg atggatgctg
     1321 tgccagaaac ctttggtagt atttgggttg ctgtatctat tttattagtg ttgttggtgt
     1381 ttgttgggat gattatggac ccctttggtg cggttatttt agtatcggcc actgttgcac
     1441 caatcgccta taaaaatggc attgatcctg ttcacttttg gatgattgta ttaacagctt
     1501 ttgaatt
//
LOCUS       DAOQMB010000566         1505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_66868_length_1505_cov_7.192414, whole genome shotgun sequence.
ACCESSION   DAOQMB010000566 DAOQMB010000000
VERSION     DAOQMB010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66868_length_1505_cov_7.192414"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLY05_12095"
     CDS             <1..1175
                     /locus_tag="PLY05_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC04082.1"
                     /translation="NGIFLLPPSQYKDVNEWVLAEGADGLNKMIDEAIQTKKAVPPSQ
                     DGTASVDDDLARESIDDGQAKSTTKPMLNVVTVGELVQMDIPPRENLLAPWLPMQGLA
                     MVYAPRGIGKTFFALNVAYAVASGGEFLCWQATQAYPVLYIDGEMPVVSMKERLIDVI
                     SMHDSIASMDALRIITPDLQQDYMMPDLATLEGQQALEPYLQGIKLIVIDNISTLCRS
                     GRENESEGWIVVQEWALRQRAAGRSVLFVHHAGKGGNQRGTSKREDVLDTVINLKRPI
                     DYEPSHGASFEIHFEKTRGFSGEDAEPLSCQLGHDQHGQAAWLYSRLEDSTFDKVVNL
                     INEGLSQAEIASELDINKSNVSRHVKKARLQGLIKDDKKQAKPVNSANYSKVKDGE"
     gene            1181..>1505
                     /locus_tag="PLY05_12100"
     CDS             1181..>1505
                     /locus_tag="PLY05_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04083.1"
                     /translation="MNLKELALQNRKSCAVALPSNRTAQLLDDLSPNMRNSQRNHSAT
                     DSLQPLKNIDSICNLQRNTSATGQNLTAQLLPQKTPQKLRTKNAELHTKSEEKSPVFG
                     GGNSVI"
BASE COUNT          466 a          311 c          349 g          379 t
ORIGIN      
        1 taaacggtat ttttttactt cctccgagcc agtacaaaga tgtcaatgaa tgggtacttg
       61 ctgagggtgc agacggatta aacaagatga ttgatgaagc gatacaaaca aaaaaggccg
      121 taccccctag ccaagatggt acagcctctg tagatgacga tttagcaagg gaaagtatag
      181 acgatggtca agccaagtca actactaagc caatgttaaa cgtagtcact gtaggcgaac
      241 ttgtacaaat ggatatacca ccgagagaga acttgcttgc gccatggttg cctatgcagg
      301 gcttggcaat ggtgtacgcg cctcgcggta taggcaagac cttttttgct ttaaatgtgg
      361 cctatgccgt tgcaagtggt ggtgagtttt tatgttggca agccacacaa gcctatcctg
      421 ttttatatat tgatggagag atgccagttg ttagcatgaa agaacggttg attgatgtta
      481 tcagtatgca cgattctatc gctagtatgg acgctttacg catcattacg cccgatttac
      541 agcaagatta catgatgccc gacttggcta cccttgaggg tcagcaagcc ctagagcctt
      601 atttacaagg aattaaactg attgttattg ataacattag taccctttgc cgtagtgggc
      661 gtgagaatga atctgagggt tggatagttg tacaggaatg ggctttacgc caacgagctg
      721 caggccgtag cgtgttattt gttcaccatg caggaaaagg cggcaatcaa cgcggcacta
      781 gcaagcgcga agatgtactt gatacggtca ttaatcttaa acgacctatt gattacgaac
      841 cctcacacgg tgcaagtttt gagattcatt ttgaaaagac acgcggtttt agtggtgaag
      901 atgccgagcc gttatcttgc caactaggac acgaccaaca cggacaggcc gcttggttat
      961 acagccgatt agaagattca acttttgata aagtggtgaa cttaattaac gagggattat
     1021 cacaagctga gattgccagt gagttagata ttaacaagtc taatgtatca agacacgtta
     1081 aaaaggcacg tttacaaggc ttaattaagg acgacaaaaa gcaagcaaag cctgtcaatt
     1141 cggccaatta ctctaaagtc aaagatgggg agtaatggcc atgaatttaa aagagcttgc
     1201 cctccaaaat cgaaaaagtt gcgcagttgc gttgcctagc aaccgaaccg cgcaactttt
     1261 agatgattta agcccaaata tgcgcaactc acaacgcaac catagcgcaa ctgacagctt
     1321 gcaaccattg aaaaatatag attctatatg caacttacag cgcaacacta gcgcaactgg
     1381 tcaaaattta accgcgcaac ttttaccaca aaaaacgccc caaaagttgc gcactaaaaa
     1441 cgccgagttg catacaaaaa gcgaagagaa aagccctgtt tttggtggcg gtaattctgt
     1501 cattt
//
LOCUS       DAOQMB010000567         1499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_67400_length_1499_cov_111.717452, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000567 DAOQMB010000000
VERSION     DAOQMB010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1499
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67400_length_1499_cov_111.717452"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLY05_12105"
     CDS             <1..303
                     /locus_tag="PLY05_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC04084.1"
                     /translation="GLNYHKKQFKQLLRMIALGQVAHLILTHKDRLLRFGAEIIFQLC
                     HIHQTNVTILEQDHELNLEKSLVADVLELMTVFSARLHGSRSHKNKKAVKESVVMS"
     gene            341..>1499
                     /locus_tag="PLY05_12110"
     CDS             341..>1499
                     /locus_tag="PLY05_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HNC04085.1"
                     /translation="MLSTAHQIELKPNNKQANYFARACGVARFSFNWGLEQWQHQYQA
                     HVQDNSLPKPNEQALRKHFNAVKKQEFGFVLEVTKYAAQQALKNVGTAFKRFFNGEAA
                     YPQFHKKHHHDSFYIGNDQFKVKDKQVWIPNLGWVRLKEAFKYQGKGKINNAVISRQA
                     DRWFVSISVSVEQKPPRQAVEKQDCVGVDLGISRLATCSDGHQADNPKALSKKLKKLK
                     RLSQALSRKVKGSHNRHKAKMQLSKLHQRIKHIRHDALHKLSYYVTTRFNMVVLEDLN
                     VQGMMKNRKLSRAIADVGFYELRRQVEYKAKYYGSRVVYADRFYPSSKLCSVCGFKHE
                     GLTLKDREWDCPECGIHHDRDVNAALNLRQLGLAKIAASSTVTACGEFGSGV"
BASE COUNT          493 a          269 c          340 g          397 t
ORIGIN      
        1 gggttaaatt atcataaaaa gcaatttaaa caactactta gaatgatagc cttaggtcaa
       61 gtagcgcatc ttatcttaac gcataaagac agattattgc gttttggggc tgagattatt
      121 tttcagttgt gtcacattca tcaaaccaac gtgactattt tagagcaaga ccacgagctt
      181 aacttagaaa agagcttagt ggccgatgtg ctagagctaa tgaccgtatt ttcagcacgt
      241 ttacacggtt cacgctcaca taaaaacaaa aaagccgtga aagaatcagt ggttatgagt
      301 tagaataata aaattcaccc tattaaatgg cttgtggttt atgctcagca ccgcccatca
      361 aatagaactt aaacccaaca acaagcaagc caattatttt gcgcgtgctt gtggggtggc
      421 acgttttagt tttaattggg gcttagagca atggcaacac caatatcaag cccatgtaca
      481 agataacagt ctccccaaac ccaacgaaca agctctcaga aaacacttta atgcggttaa
      541 aaaacaagag tttggttttg tgttagaggt gactaaatat gcagcacaac aagccttaaa
      601 aaacgtaggc acagcgttta aacggttttt taatggtgaa gctgcgtatc cgcaatttca
      661 taaaaaacat catcacgact cattttacat aggcaacgac caatttaaag tcaaagacaa
      721 acaggtatgg ataccgaatt tgggttgggt gagattaaaa gaagcgttta aatatcaagg
      781 gaaaggcaaa ataaacaatg ccgtcatcag ccgacaggcg gatagatggt ttgtgagtat
      841 atcggtgagt gtagaacaaa agccaccacg ccaagcagtc gagaaacaag actgtgtggg
      901 tgtggatttg ggaattagtc gcttagcgac ctgttcggat ggtcatcaag cggataatcc
      961 aaaggcattg tctaaaaagc tcaaaaagtt aaaacggtta agtcaagcct taagccgtaa
     1021 ggttaaaggc tcacacaatc ggcataaagc gaaaatgcag ttatctaaac tgcatcaacg
     1081 gataaaacac attcgtcacg atgctttaca caaactgtct tactatgtaa ccacacggtt
     1141 taacatggtg gttttagaag atttgaatgt acaaggcatg atgaagaatc gaaaattatc
     1201 gagagcgatt gctgatgtcg gtttttatga attaaggcga caggtcgaat acaaagccaa
     1261 gtattatggg tcaagggtgg tgtatgcaga ccgtttttat ccgagttcta aattgtgttc
     1321 ggtgtgtggt tttaagcatg aaggcttgac gttaaaagac cgtgaatggg attgtcctga
     1381 gtgtggcatt caccatgata gagatgtaaa tgctgcctta aatttaaggc agcttggttt
     1441 agcaaaaatc gccgcgagtt caacggtgac cgcttgtgga gagtttggct ctggcgttt
//
LOCUS       DAOQMB010000568         1495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_67767_length_1495_cov_15.795833, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000568 DAOQMB010000000
VERSION     DAOQMB010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67767_length_1495_cov_15.795833"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLY05_12115"
     CDS             <1..513
                     /locus_tag="PLY05_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNC04086.1"
                     /translation="VQKLTRTAQVLLDEATYPVLMHQVIDDRSARNDQQINKLFLYPK
                     EEQAILNALNYGLPYPTMPTATANHASHSATTPSENRSLGIVQSVKEGGFGFITPDED
                     MENIFFHYSEVKNRNFTDLHIGDRVSYILGRNDKGYCATQIMVENQTDSVFYNDPPYD
                     DEPFLAYDED"
     gene            complement(498..953)
                     /locus_tag="PLY05_12120"
     CDS             complement(498..953)
                     /locus_tag="PLY05_12120"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC04087.1"
                     /translation="MLHTLNTLPWTVTPAYIDCVTVIPAHQDGFAHTNNVTYLQWLEK
                     AAWGHSQALGLDIAAYYRLGCGCVVRKHELNYLLATHVGDELAIGTWISANDGKLSTV
                     RDYQIIRLADNKTVLTGQTHFITVDMTTGKPKRMPPEFVAAYLPVQSSS"
     gene            complement(1126..1401)
                     /locus_tag="PLY05_12125"
                     /pseudo
     CDS             complement(1126..1401)
                     /locus_tag="PLY05_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020190138.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(1382..>1495)
                     /locus_tag="PLY05_12130"
     CDS             complement(1382..>1495)
                     /locus_tag="PLY05_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNC04088.1"
                     /translation="RNPKTGEEIPITARRVVTFKAGQKLRQRVDAYARTQS"
BASE COUNT          450 a          319 c          285 g          441 t
ORIGIN      
        1 gtgcaaaaac tgactcgtac tgcacaagtt ttattagatg aagccactta tcctgtctta
       61 atgcaccaag taattgatga tcgttctgca cgcaacgatc agcaaatcaa taaattattc
      121 ctatatccta aagaggaaca agcgattcta aatgccttaa attatggctt accctatcct
      181 actatgccaa cagccacagc caatcacgct tcacactcag ccactacccc gtcagaaaat
      241 cgtagtttag gtatagtaca aagtgttaaa gaaggtggat ttggttttat tacgccagat
      301 gaagatatgg aaaatatctt ctttcattat agtgaagtta aaaatcgtaa ttttaccgat
      361 ttacacattg gtgatcgtgt aagttatatt ttaggtagaa atgataaagg ttattgtgct
      421 actcaaatca tggtcgaaaa tcaaactgac tcggtatttt ataacgaccc accttatgat
      481 gatgaacctt ttttagctta tgatgaggat tgaacaggca gataagccgc cacaaactct
      541 ggcggcattc gttttggttt gcctgtggtc atatcaacag taataaaatg ggtttgacct
      601 gtcagtacag ttttattatc agccaagcga ataatttgat aatcacgaac ggttgatagt
      661 ttaccgtcat tggcactaat ccaagtacca atcgctagct catcgccaac atgtgtggcc
      721 aataaatagt tcagttcgtg tttacgtact acacaaccac agcctaaacg ataataggca
      781 gcgatatcta aacctaaagc ttgagaatgc ccccacgcag ctttctcaag ccattgtagg
      841 taagtaacat tattagtatg ggcaaaccca tcttgatggg cagggataac tgtcacacaa
      901 tcaatatagg caggtgtgac agtccatggc agggtattaa gcgtgtgtaa cattaccacc
      961 taacaagact aaaacagctt ctaactctct aatcatatcc ttaatgacca cattgagcat
     1021 attttggctg atttgaactt gataagccaa catagcctct gacgggctag gttcaatgct
     1081 gggtgctgat tcagcaacaa tcgcttccat gactttagcc atcggtcgtg cgtgttctgt
     1141 ttgtaactgc aaacgtgcac cactaatggt aaaaccttgt tcatataaca aactacgaat
     1201 ttgacgaatc atcagcacat cttcgcgttg gtaataacgg cgattaccac gtcgcttcat
     1261 tgggcttagc tgttcaaact cttgttccca ataacgtaac acatgcggct taacattaca
     1321 caaatcaccc acttcaccaa tggtaaaata gcgcttgttg ggaatcgcag gtaataacgc
     1381 gttatgactg ggttctagca taagcgtcta ctcgttgtct tagtttttgt cctgctttaa
     1441 acgttactac acgacgtgca gtaattggaa tttcttcgcc tgtttttgga ttacg
//
LOCUS       DAOQMB010000569         1494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_67857_length_1494_cov_12.302988, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000569 DAOQMB010000000
VERSION     DAOQMB010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67857_length_1494_cov_12.302988"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1447
                     /gene="mltF"
                     /locus_tag="PLY05_12135"
     CDS             68..1447
                     /gene="mltF"
                     /locus_tag="PLY05_12135"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HNC04089.1"
                     /translation="MTGKKALPAVTIVKGFFQQYWRRVVPLCLLTIISLQLHPSKQYT
                     VDEFLERGSLRVIGIAGPTTFLPHGEHGVRGLQYELLRQFANDLNIRLEIEQAPNAEA
                     VITALEKGEADLGITGLANNDPRLAGLEISKPYLSTTQQLIQRSGTRLSPSEAKIAVS
                     QNSAEADIIEQLQPEADVVQLRYATPDELLEEVNQGQVDYVVMNDSEFAARRTAFPQL
                     SLKKQLTTSQLSWTIRPRDKQLLRFANRFLTLTEQDGSLQRLNNFYSQGDTFNAFGVK
                     TFQKDIQQRLPLYQAQFKKQANTHDMDWRLLAAIAYQESKWDVNAVSPTGVTGIMMLT
                     KNTAQEMGITNRNNPQQSILAGSAYYQRMIDKIPDTVHEPDRTWMALAAYNMGYRHVL
                     KARELSLKQGDDPDKWLDVSRHLRQLPRAGQALVYVQEVRRYYDALLLQDSRSHWMAS
                     LEKKWVVAE"
BASE COUNT          450 a          305 c          310 g          429 t
ORIGIN      
        1 ttaaagttgc cgctaaattt gcagtttaga ttatttttta atcaataaaa tattgggggt
       61 gtgatacatg acaggcaaaa aggccttgcc cgccgtcact atcgttaaag gtttttttca
      121 gcaatattgg cgacgtgttg tgcctttgtg cttattaacg ataattagct tacagctaca
      181 cccctccaaa caatacactg ttgacgaatt cctagaacgt ggcagtttac gcgtgattgg
      241 tattgcaggg ccaaccactt ttttaccaca tggtgaacat ggggtacgcg gcttacaata
      301 tgagttatta cgtcaatttg ccaacgattt aaatatccgt ttagagattg aacaagcccc
      361 caatgccgaa gcagtcatta ctgctttaga aaaaggtgaa gccgatttgg gtattactgg
      421 ccttgctaat aatgatccac gtttagcagg tttagaaatt agtaaaccct atttaagtac
      481 cactcaacaa ctgattcaac gctctggtac acgtttaagc cctagtgaag ccaaaattgc
      541 cgtcagtcaa aatagtgctg aagccgatat aattgaacaa ttacagcccg aagcggatgt
      601 ggtacaactt cgttatgcca cgcctgacga actcttagaa gaagtcaatc aaggtcaggt
      661 tgattatgta gtgatgaacg acagtgagtt tgcagctcgt cgcaccgctt ttccgcaatt
      721 aagtttaaaa aaacaattaa ctactagcca actgtcatgg acaattcgtc ctcgtgataa
      781 acagttatta cgttttgcca atcgcttttt aacactaact gagcaagatg gttctttaca
      841 acgtttaaat aacttttata gccaaggcga tacctttaac gcttttgggg tcaaaacctt
      901 tcaaaaagat attcaacaac gtttgccgtt gtatcaagcg caatttaaaa aacaagccaa
      961 cacccacgat atggattggc gtttattagc cgcgattgcc tatcaagaat ctaaatggga
     1021 tgtcaatgcg gtgtcaccca caggtgtgac aggtattatg atgctcacca aaaataccgc
     1081 ccaagaaatg gggattacta atcgcaataa tcctcagcaa agtattttgg caggttcagc
     1141 ttattatcaa cggatgattg ataaaattcc tgatactgtg catgaacccg atagaacgtg
     1201 gatggcctta gccgcttata atatgggcta tcgacatgtg ttaaaagcac gtgaattaag
     1261 cctaaaacag ggtgatgatc ctgataaatg gctggatgtg agtcgccatt tacgtcaatt
     1321 accgcgtgca ggtcaagcct tagtgtatgt acaagaagtc cgtcgttatt atgatgcctt
     1381 gttattacaa gatagccgta gtcattggat ggccagttta gaaaagaagt gggtcgtggc
     1441 ggagtaaact cagaccattt tctttttagc ctctcgcctc ctcctaaaaa aatc
//
LOCUS       DAOQMB010000570         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68043_length_1492_cov_29.297147, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000570 DAOQMB010000000
VERSION     DAOQMB010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68043_length_1492_cov_29.297147"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..513
                     /locus_tag="PLY05_12140"
     CDS             217..513
                     /locus_tag="PLY05_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04090.1"
                     /translation="MAARQWTEEQRKAQAEKIRQHKIWLQSTGPHTEEGKKKCSQNAL
                     KTGEYTADKVAERKRKAANKKLYGAFCCYGDKRYSFNRSFKYSGFFRTFASFGK"
     gene            653..1288
                     /locus_tag="PLY05_12145"
     CDS             653..1288
                     /locus_tag="PLY05_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04091.1"
                     /translation="MSGYYNRKMTTDEAKKIDIRELQRRQSLYCGNTGELFWHNARAK
                     EQVGLIRFEVKPDCLVFSYRAKRHNSQYWQDYEVIAPLTYTPCNYGNSRVWFKCPRCS
                     KRVAILYINAQIACRTCQRLNYASQQQTKGMCQDQDRMNKIRQKLNWPLYQDVLFRTK
                     PKGMHYKTFYHLVKEHDFYELSYWESFGATMPSLFAKLNDIKQSLKRAANK"
     gene            complement(1410..1486)
                     /locus_tag="PLY05_12150"
     tRNA            complement(1410..1486)
                     /locus_tag="PLY05_12150"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1450..1452),aa:Met,seq:cat)
BASE COUNT          497 a          282 c          318 g          395 t
ORIGIN      
        1 cataggtaac ggtaaatgca acgctacaga aaaaagcaac ggtaaggtga cggtaaggca
       61 acggtaattg ttttagcgtt accccccttg caagccttga taacactagc ttttagatta
      121 taaaaacgga cggtgtaacc caaagaaaag gcaacggtaa agcgtgttat tttctaattt
      181 aataacgaac tgttagcttt attttggagg tacaacatgg cagctagaca gtggacagag
      241 gaacagcgca aggcgcaagc tgaaaaaata cgccaacata aaatatggct acaatcaaca
      301 gggccacaca ctgaggaggg caaaaagaaa tgttctcaaa atgcgctaaa gactggcgag
      361 tacacagccg acaaagtggc agagcgtaaa cgtaaggctg ctaataaaaa gttgtacggt
      421 gctttttgtt gttatggcga taaacgatat agctttaata ggtcatttaa gtattcaggc
      481 ttttttagaa cctttgcatc ttttggaaag tagtccgcac tgagtacgca acaggagaca
      541 acatcacgca ccttgtaaac acacgcaaag ccttatatac caatgcttag agctattttt
      601 gtgtagttgc catgctataa tttgcctaat gatttaggcg gattaaaata aaatgagtgg
      661 ctactacaac cgtaaaatga ccaccgatga agcaaaaaag attgatattc gcgagctgca
      721 gcgtaggcag tctttatatt gtggcaatac aggcgagtta ttttggcata acgcaagggc
      781 taaagagcaa gtaggcctta ttcgttttga ggtaaaacct gattgtctag tatttagtta
      841 ccgagcaaaa cgacacaata gccaatactg gcaggattat gaagttatcg cgcctttgac
      901 ttatacgcca tgcaattatg gcaatagccg cgtatggttt aagtgtccac gatgtagcaa
      961 gcgtgtcgcc attctttata ttaatgcaca aattgcctgc cgtacttgtc agcgtttgaa
     1021 ctatgcaagc caacaacaaa ctaaaggtat gtgccaagac caagaccgca tgaataaaat
     1081 ccgacaaaaa ttaaactggc cactttatca agatgtgttg tttcgtacta aacccaaagg
     1141 tatgcactac aaaacatttt atcatttagt caaagaacat gatttttacg agctgagtta
     1201 ttgggaaagt tttggcgcaa caatgccgtc tttatttgcg aagctaaatg atattaagca
     1261 aagtctaaaa agagctgcta ataagtaagg ttttagggta aaaagcgata aattgctttc
     1321 tattgtagcc cccgtgtagc cccgagaaaa attacaaaaa ataaaaaagc cttacatttc
     1381 tgcaaggctt tgatatttaa gtatttattt ggtggctatg gctggacttg aacctgcgac
     1441 cccagcatta tgagtgctgt gctctaacca actgagctac atagccatct ac
//
LOCUS       DAOQMB010000571         1492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68044_length_1492_cov_24.352122, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000571 DAOQMB010000000
VERSION     DAOQMB010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68044_length_1492_cov_24.352122"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLY05_12155"
     CDS             <1..195
                     /locus_tag="PLY05_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNC04092.1"
                     /translation="NGKAKLEVIIGILRVLGKLENVDDFLPELPFSPIELLKLEGKKR
                     QRATRNSALEVKHAEEDTDW"
     gene            189..>1492
                     /locus_tag="PLY05_12160"
     CDS             189..>1492
                     /locus_tag="PLY05_12160"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNC04093.1"
                     /translation="MVTHKVARVLLWGRTVGALSYNNDTGLCAFQYDPSWIKTGIEIS
                     PIRLPLSPQIYQFPTLSKATYKGLPAAFADTLPDDFGNAIIDAWLARTGRDHETFSAL
                     ERLLYTGKRGMGALEYAPSLEKQFNTSSPVELESLIQIAQTILDQRAQLVKPFMPSQQ
                     QDALSAILQVGTSAGGARPKAVIGINQARTEIRSGQVDLPNGFEHYLLKFDGVVEHSN
                     AKETFGDPQGFGRMEYAYYLMAQAAGIHMSPCELLIDGDRAHFMTKRFDRIGNKKIHY
                     QSLCAMDHADYKKPGHYSYEQVFSLMRALKLSKSAALEMYRRMVFNIVARNHDDHTKN
                     TGFILNAQHQWELSPAFDIAYSYKPDSPWVNSHQLSLNGKRDNFVRNDLLLPAEKFRK
                     EAEAIIEEVIDVVAQWPKYAKEAGVFPEFAQRIQRHHRLKFI"
BASE COUNT          450 a          272 c          333 g          437 t
ORIGIN      
        1 aatggtaagg ccaaacttga agtcattatt ggtattttaa gggtattggg taagttagaa
       61 aatgtagatg attttttacc tgaactaccc tttagtccaa tcgaattact aaagctagag
      121 ggaaaaaaaa gacaacgtgc tactcggaac agtgcgcttg aagtgaaaca cgcagaggag
      181 gatacagatt ggtaacacat aaggttgccc gtgttctgtt atgggggcgt actgtgggtg
      241 ctttatcgta taataacgat acaggactat gtgctttcca atatgaccca tcatggataa
      301 aaacagggat tgaaataagt ccgattaggc tgccattatc tccccaaatt tatcagtttc
      361 ctacgctatc aaaagcgaca tataagggtt tgccagccgc ttttgccgat accttacccg
      421 atgattttgg taatgcgatt attgatgcat ggctagcacg aacaggtaga gaccacgaaa
      481 catttagtgc cttagaacgt ttgttatata caggtaagcg tggtatgggc gcattagaat
      541 atgcaccctc tctagagaaa caattcaata cgtcctctcc tgtggagcta gagagcctga
      601 tccaaatagc acaaactatt ttggatcagc gagcacagtt agtaaaacct tttatgcctt
      661 ctcagcaaca agatgcgctt tcggcgatat tgcaagtggg tacatctgcg ggtggtgcta
      721 gacctaaagc ggttattggt attaatcaag cacgaacaga gatccgcagt ggtcaggtgg
      781 atttgcctaa tggttttgag cattatttat taaagtttga tggtgttgtt gaacattcta
      841 acgccaaaga aacgtttggt gatccacaag gttttgggcg tatggagtac gcttattatt
      901 taatggctca agcagctggc attcatatgt cgccttgtga gttgttaatt gatggcgatc
      961 gagcgcattt tatgacaaaa cgttttgatc gaataggcaa caaaaaaatc cattatcaat
     1021 cgctatgcgc aatggatcat gccgattata aaaaaccagg ccattatagc tatgaacaag
     1081 ttttttcatt gatgcgtgcg cttaaactca gtaaatccgc tgctttagag atgtataggc
     1141 gtatggtatt taatattgtc gccagaaatc atgacgacca tactaaaaac acaggattta
     1201 tcttaaatgc ccaacaccaa tgggagcttt caccagcatt tgatattgct tatagctaca
     1261 agcctgattc gccatgggta aattctcatc agctatcatt aaatggtaag cgagataatt
     1321 ttgtgcgcaa tgatttgtta ttacctgccg agaaatttcg taaagaagct gaagctatta
     1381 ttgaagaagt cattgatgtt gttgcacaat ggcctaaata cgcaaaagag gctggtgtgt
     1441 ttcctgagtt tgctcaaaga attcagcgtc atcatcggct aaagtttata ta
//
LOCUS       DAOQMB010000572         1491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68141_length_1491_cov_19.096797, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000572 DAOQMB010000000
VERSION     DAOQMB010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68141_length_1491_cov_19.096797"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..720
                     /locus_tag="PLY05_12165"
     CDS             13..720
                     /locus_tag="PLY05_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HNC04094.1"
                     /translation="MAKVILIANQKGGVGKSTIATTLGTGLKRLGHRVLLLDADSQQS
                     LFKWRASAEGLAHEQDIPWVERWDSPLIAEKIKKEKQHYDFIIIDSASNLGQKGDATQ
                     KIILGAIKSADLILTPVGPSPFDVDGTEDFINLLRDIWERFGEQKPPVYFLINGVQVG
                     TTLGREVGEYVANTYQLPVLNTKLHMRQAYRQCVFDGSSIFHLGDAQSKANASAFVDE
                     VLKLSMLNSDIKVNNHE"
     gene            713..>1491
                     /locus_tag="PLY05_12170"
     CDS             713..>1491
                     /locus_tag="PLY05_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNC04095.1"
                     /translation="MSKKGFFSNRKTPTTATNNAATVNETRFDLAEFERDVIAEARAN
                     NQIAQIAKDLIDPDPNQPRSYFDPVAMDELRLSIEQNGLIQPIVVRQMGERYQLIAGE
                     RRWRACLSIDSIINIEAVIRNDVEPLTILLLQIAENNHRQSMTPMDTARAYQRVKELS
                     NDSQKQAASKLGISESQMSITLGLIKAPPVIQNLAMSGKIKDITTLNLVNRLYEQNAL
                     AAEQVVQEILEERIESGNVRKQVNLVLQQNKGKIEHAQVEK"
BASE COUNT          480 a          277 c          322 g          412 t
ORIGIN      
        1 tgaggtggat ttatggcaaa agtgatttta atcgccaatc aaaaaggcgg tgtgggtaag
       61 tcaaccattg ccacaacatt agggacaggc ttaaaacgtt tagggcatcg tgtgttgtta
      121 ttagatgcag actcgcaaca atccttattt aaatggcgcg ctagtgcgga aggtttggcg
      181 catgagcaag atattccgtg ggttgagcgt tgggattcac ccttaattgc cgaaaaaatt
      241 aaaaaagaaa aacaacatta tgattttatt atcattgata gtgccagtaa tttaggtcaa
      301 aaaggagatg ctacccaaaa aattatttta ggtgcaatca aatccgctga tttaattttg
      361 acacctgttg gcccctcacc ttttgatgtt gatggcaccg aagactttat caatttattg
      421 cgtgatattt gggagcgttt tggtgaacaa aaaccacccg tttatttttt aattaatggt
      481 gtgcaagtgg gaacaaccct aggccgagaa gtcggtgaat atgtcgctaa tacttaccaa
      541 ttacctgtgt taaataccaa actacacatg cgccaagcct acagacaatg tgtgtttgat
      601 ggcagcagta tttttcattt gggtgatgcc caaagcaaag ccaatgcctc tgcatttgtc
      661 gatgaagtgt taaaattgag catgctcaat tctgacatca aggtaaataa ccatgagtaa
      721 aaaaggcttt ttttccaatc gcaaaacacc aaccacggca acaaataatg cggctacggt
      781 gaatgaaaca cgttttgatt tggcagaatt tgaacgtgac gtgattgccg aagcacgtgc
      841 taataatcaa attgcacaaa ttgctaaaga cttaattgac ccagacccca atcagccgcg
      901 cagttatttt gaccctgtgg ccatggatga gctacgacta agtattgagc aaaatggttt
      961 aattcaaccg attgttgtta ggcaaatggg agagcgttat caactgattg caggtgagcg
     1021 acgttggcga gcctgcttgt ctattgatag cattattaat attgaagcag tgattcgtaa
     1081 cgatgtagag ccattaacta ttttgctgct gcaaattgct gaaaataatc atcgtcaatc
     1141 catgacgcca atggataccg ctcgcgctta tcaacgtgtt aaagaattat caaacgactc
     1201 gcaaaaacaa gctgccagca agttgggtat ttctgaaagc caaatgtcga ttaccttggg
     1261 gctaattaaa gcaccacctg tgattcaaaa tttggcaatg agtggcaaaa ttaaagatat
     1321 taccacctta aatttagtca atcgtttgta tgaacaaaat gcactggcag ccgaacaagt
     1381 tgtacaagaa attttggaag agcgtattga atcaggtaac gtccgtaaac aagtcaatct
     1441 ggttttgcaa caaaacaaag gcaaaattga gcatgctcaa gtcgagaaaa a
//
LOCUS       DAOQMB010000573         1488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68437_length_1488_cov_6.090719, whole genome shotgun sequence.
ACCESSION   DAOQMB010000573 DAOQMB010000000
VERSION     DAOQMB010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68437_length_1488_cov_6.090719"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLY05_12175"
     CDS             complement(<1..295)
                     /locus_tag="PLY05_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04096.1"
                     /translation="MSKSRMVINCLSADDKLKKQIKSIVHVLDLVGGLKKSWVYADDN
                     ASVHLIDMDDEDNWDILVASSGLLIIAVSSNPELLSGQKYSLTKPIRNQTLQAI"
     gene            836..1231
                     /locus_tag="PLY05_12180"
     CDS             836..1231
                     /locus_tag="PLY05_12180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNC04097.1"
                     /translation="MPTIFEKIIAGQAPCHKIAETKHALAFLDIRPMSKGHTLVIPKR
                     HSLDLLAIEQQDLFAVMDLAQQVAKRQMTVLSADGITVRQHNRKAGGQDVFHFHVHVI
                     PRWNNQKLNHPAPLASQQELASLALLLQF"
     gene            1308..>1488
                     /locus_tag="PLY05_12185"
     CDS             1308..>1488
                     /locus_tag="PLY05_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04098.1"
                     /translation="MKDLNAMAQAFKALSHPNRLQMYLELLELREKIVSSGSCEQKEC
                     CFGLSEFMHKLNIGAP"
BASE COUNT          498 a          307 c          239 g          444 t
ORIGIN      
        1 atatcgcttg taatgtttga tttctaatgg gtttggtgag cgaatacttt tgaccactca
       61 aaagctctgg gttactactg acggcaataa tgagcagacc tgaacttgct accaagatgt
      121 cccagttatc ttcatcatcc atatcaatca gatgaacaga ggcattatca tctgcatata
      181 cccaactttt ttttaagccg ccaaccaaat ctaaaacatg cacaatcgat ttgatttgtt
      241 tttttagttt atcatcggca gagaggcaat tgataaccat gcgcgattta gacataaccc
      301 ttcctaatca ttatttgaca caagtgttta ctcaacaatc acccaacatt cgggaacatc
      361 acccgcagca agcatcaaaa aaacacataa acacgaggaa gatatgagcg tttttatact
      421 gtcaatcgat tgtcgttttt tcatcatcat accaccttaa agtttttctt attattgctt
      481 ctacttgctt gtacaatcag catgtaagct aaagccttca acatttgccg agttataaaa
      541 aacttaataa aatcaaacaa aaagaaatgt cagtttattg aaaataacag actctcataa
      601 aaactgcata tacggtttga gcgtatttta aaaaatcaag cagcagtagc ttttgtcaca
      661 ataaccgtac aaaatatttt gagtggctat gtataacata cttaaagttg taaacaatac
      721 tcaatattat gtgaaataaa tcacaaaatt gtttaaagac tcaacacgct taccgcacct
      781 gcacaaatca tcaaaagccg atacattgtc atatttatat gagaggataa acctaatgcc
      841 tactatcttt gagaaaatca ttgcaggcca agcgccttgc cacaaaattg ccgaaacaaa
      901 acatgcttta gcctttttgg atattcgacc gatgagtaaa ggccatacgc ttgttattcc
      961 caaacgtcat agccttgatt tattagccat tgaacaacaa gacttatttg cagtcatgga
     1021 cttagcgcaa caagtggcca aacgtcaaat gactgtttta tctgccgatg gcatcaccgt
     1081 aagacaacat aaccgtaaag caggcggtca agacgtgttt cattttcatg tccatgttat
     1141 tcctcgctgg aataatcaaa aactaaatca ccccgcacct ctcgccagcc aacaagaatt
     1201 agcctcttta gccctgttac ttcaatttta agcaagatat ttcgatactt gtcaaattat
     1261 aattatgtta tactatgagg aatgatattt tgttgtagag tataaagatg aaagatttaa
     1321 atgctatggc tcaagctttt aaagccctct ctcacccaaa ccgattacaa atgtatttgg
     1381 agctattaga gctgcgcgaa aaaattgtct catcgggtag ctgcgagcaa aaagaatgtt
     1441 gttttggctt gtctgaattt atgcacaaac tcaacattgg tgcaccaa
//
LOCUS       DAOQMB010000574         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68830_length_1484_cov_15.897131, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000574 DAOQMB010000000
VERSION     DAOQMB010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68830_length_1484_cov_15.897131"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1356
                     /gene="prpF"
                     /locus_tag="PLY05_12190"
     CDS             160..1356
                     /gene="prpF"
                     /locus_tag="PLY05_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HNC04099.1"
                     /translation="MSNIQQIKIPATYMRGGTSKGVFFRLQDLPESCQVAGAARDNLF
                     MRVIGSPDPYAKHIDGMGGATSSTSKCVILSKSSQPEHDVDYLYGQVSIDKAFVDWSG
                     NCGNLSTAAGAFAIHAGLVDAARIPQNGVCVVRIWQANINKTIIAHVPVTNGQVQELG
                     DFELDGVTFPAAEIVLEFLDPADEGEGDAGGSMFPTGNLIDDLDVPNIGTLKATMITA
                     GIPTVFVNAEDIGYTGTELQEAINGDTKALAKFEAIRVAGALRMGLIKHPDEALTRQH
                     TPKIAFVAQPKDYISSSGKQVKASDVDLLVRALSMGKLHHAMMGTAAVAIGTAAAVSG
                     TLVNLAAGGGERQAIRFGHPSGTLRVGAEAKQVNGEWIVTKAIMSRSARIIMEGWVRV
                     PEDIIV"
BASE COUNT          442 a          277 c          351 g          414 t
ORIGIN      
        1 gattatggtt gtgagccaac ggcaggtatc ttagtggggg tcacatcaga gcgttatatt
       61 ttagctcgcc aagacccaga gctaggtatc ttgcatgttc attttccacg tcagggttat
      121 aagttaaaaa cttaaagtat tgaatttaag agaactgtta tgtcgaatat ccagcaaatt
      181 aaaattcctg caacttatat gcgtggtggt acgagtaaag gcgtattttt tcgtttacaa
      241 gatttacctg aatcttgcca agttgcagga gcagcccgag ataatttatt tatgcgcgtg
      301 attggtagcc cagaccctta tgctaaacat attgatggta tgggtggtgc aacatcaagc
      361 accagtaaat gtgtgatttt gtcaaaaagt agccagccag aacacgacgt tgattattta
      421 tatggccaag tttccatcga caaagctttt gtcgattgga gtggaaactg tggcaattta
      481 tcgaccgctg cgggagcatt tgccattcat gcgggtttag ttgatgctgc acgtattcct
      541 caaaatggcg tttgtgtggt cagaatttgg caagccaaca ttaacaaaac cattattgct
      601 catgtacctg tcacgaacgg ccaagttcaa gagctaggtg attttgaatt agatggtgtg
      661 acttttcctg ctgccgaaat cgttttagag tttttagatc cagcggacga aggtgagggc
      721 gatgcgggtg gctcaatgtt cccaacggga aatttaattg acgatttaga tgtccccaat
      781 attggcacgt taaaagcaac catgattacc gcaggtattc caaccgtctt tgtcaatgcc
      841 gaagacattg gttatacagg tacagaattg caagaagcca ttaatggtga cactaaagcg
      901 ttagccaaat ttgaagcgat tcgtgttgct ggtgctttac gcatgggttt aattaaacac
      961 ccagatgaag cattaacccg tcaacacaca ccaaaaattg cttttgttgc acagccaaaa
     1021 gattatattt catcaagtgg taagcaagta aaagccagtg atgtcgattt gttggttcgt
     1081 gccttatcaa tgggtaaatt acatcatgcc atgatgggca cagccgctgt tgccattggt
     1141 acagccgcag ccgtttcagg cacattggtt aacttggcgg caggtggcgg tgagcgtcaa
     1201 gcgatacgtt ttggtcatcc gtcaggcaca ttacgtgtcg gtgcagaagc caaacaagtg
     1261 aatggtgagt ggatagtcac aaaagcaatt atgagccgca gcgcacggat tattatggaa
     1321 ggatgggtaa gagtgcctga ggatataatt gtataaaaac taaaaaaagc ccatctaaat
     1381 aatcaaatag atgggcaaaa attgaccatt tatcgactat tacaaaaaaa ataccatgta
     1441 actgctgctt agctttggca aagtggcaaa aaaatagcat aata
//
LOCUS       DAOQMB010000575         1483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_68950_length_1483_cov_3.821429, whole genome shotgun sequence.
ACCESSION   DAOQMB010000575 DAOQMB010000000
VERSION     DAOQMB010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68950_length_1483_cov_3.821429"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PLY05_12195"
     CDS             <1..286
                     /locus_tag="PLY05_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04100.1"
                     /translation="ISLFYRIPQIIAICATKPVSGTNETTTAAGYSSKSSYELNKDSD
                     TGEVSGTATATTGAGYTATVTGSGTQTGGSVTATTGSGQSKTVSYGDQKN"
     gene            312..1049
                     /locus_tag="PLY05_12200"
     CDS             312..1049
                     /locus_tag="PLY05_12200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC04101.1"
                     /translation="MRLCFLMNNHMAFTKILVVDDDPDLRQLLKSYLTKHNYDVLLLP
                     DSTQLDKTVARFAPQLIVLDWMMPHEDGISACQRLRAKQDQTPIIMLTARDETADKII
                     GLQVGADDYVCKPFDPRELLARIEAVLRRPAFIAQHGTHAKISFWEFVIDPSLRTLKK
                     SGQLIDLSGGEFDLLLVLVRHPNRVLSREKLLTLVHGDDAEPMERAIDVQIHRLRKLI
                     EVDAVHPQIIKTVWGRGYVFSLVADGV"
     gene            1049..>1483
                     /locus_tag="PLY05_12205"
     CDS             1049..>1483
                     /locus_tag="PLY05_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04102.1"
                     /translation="MSWWPRQLWRQTALMVLIVVIITQVLSLSLLAHFRMQIVKQQGI
                     ELARGYVVMVRSALTVMSPEQLAQRLIEQKQQMPINNQASIKLIYQPHAQASDVNMPV
                     GILLLYHHLQQEWGNDNVQIAAKPQAALLIRVQGDWWLQMLTT"
BASE COUNT          437 a          284 c          341 g          421 t
ORIGIN      
        1 catctcttta ttttacagaa tccctcaaat catcgctatt tgtgcaacaa agcctgtttc
       61 tggtactaat gaaaccacaa ctgccgcagg gtattcttca aaatccagtt atgaactgaa
      121 taaagatagt gatacaggcg aagtttctgg tacagcaacc gccacgacag gtgcaggtta
      181 tacggcgact gtaacaggca gtggcacaca aacaggtggt tcagtgactg caaccacagg
      241 tagtggtcag tctaaaaccg tgagttatgg tgaccaaaaa aattaaatgc agattgcaaa
      301 gggcgcatta aatgcgcctt tgttttttaa tgaataatca catggcattt accaaaatat
      361 tagtggttga tgacgacccc gatttacgac aactattaaa aagctatctc accaaacaca
      421 attatgatgt gttgttattg ccagatagca ctcagctaga taaaacggtg gcgcgttttg
      481 ctccccaact gattgtttta gattggatga tgccacacga agatggtatt agtgcttgcc
      541 agcggttgcg tgccaaacaa gaccaaacgc cgattattat gctcactgca agggatgaaa
      601 ctgccgataa aattattggt ttgcaagtgg gggcagatga ttatgtgtgt aaaccgtttg
      661 acccacgtga gttattagcc cgaattgaag cggtattacg ccgtcctgcc tttattgccc
      721 aacatggtac tcatgccaaa attagttttt gggaatttgt tatcgacccc agtttaagaa
      781 ccctaaaaaa atcaggacaa ttgattgatt tatcaggtgg tgagtttgat ttgttattag
      841 tgttagttcg tcatcctaat cgagtcttaa gtcgtgaaaa attattaacc ttagtgcatg
      901 gtgacgatgc cgaaccgatg gaacgagcga ttgatgtgca aattcatcgt ttgcgaaagt
      961 tgattgaagt ggatgccgtc catccgcaaa ttattaaaac cgtgtggggg agaggttatg
     1021 tgttttcttt agtagcggat ggtgtttaat gagctggtgg ccacgacaat tatggcgaca
     1081 aacggcactg atggtattaa ttgtcgtcat catcactcaa gtattaagcc tatcgttatt
     1141 agctcatttt aggatgcaaa ttgttaagca acagggcatt gaattagcac gcggctatgt
     1201 ggtgatggtg agaagtgctt taacagtgat gtcgcctgag cagctagcac aacgcttaat
     1261 tgaacaaaaa caacagatgc caataaataa tcaggccagc attaaattaa tttatcaacc
     1321 acacgctcaa gccagtgatg tcaatatgcc agttggtatc ttgttgttgt atcatcattt
     1381 gcaacaggaa tggggcaacg ataatgtgca aattgctgct aagccacagg ccgccttatt
     1441 gattcgtgtg cagggcgatt ggtggttaca aatgttgacc acc
//
LOCUS       DAOQMB010000576         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_69013_length_1482_cov_13.827610, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000576 DAOQMB010000000
VERSION     DAOQMB010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69013_length_1482_cov_13.827610"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="PLY05_12210"
     CDS             <1..937
                     /locus_tag="PLY05_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HNC04103.1"
                     /translation="FLAKQNIAKGQMTLIDQAQQQGLIAALQAEFHCAKRASGGSAAN
                     SVIAFAALGGSAFYACKVAKDDNGDFYLHDLRAAGVSAPHHAQVEQGITGTCVVMVTP
                     DSERTMHTFLGITAELSPQELDLDALNKARYLYIEGYLATSPSARHAVASARALAKAQ
                     NIKTALTFSDPAMVQYARHGLTEMLEDGVDILFCNEHEAKLWANTDDLAQAIQALHQL
                     SPLLAITCGDKGAIISNKGQQITIAPFPVTALDSNGAGDAFAGAFLYGVSQQWPLADC
                     GRLASRIAAQVVSQFGPRLQTVQYSKLLQETITQQ"
     gene            945..1262
                     /locus_tag="PLY05_12215"
     CDS             945..1262
                     /locus_tag="PLY05_12215"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HNC04104.1"
                     /translation="MLRLCLLAEIPDQDARGFRTPRGEVVVVRQGLVVYGYLNRCPHI
                     GISLNFQPDVFMDLTQRYLLCANHGALFRVEDGFCFRGPCHGQSLVTMQLQIEQGVIY
                     LVD"
BASE COUNT          405 a          303 c          319 g          455 t
ORIGIN      
        1 ttttttagcc aaacaaaata ttgccaaagg ccaaatgacg ctaattgacc aagcgcaaca
       61 acaaggctta attgctgctt tacaagccga atttcactgt gccaaacgtg cttctggtgg
      121 ttcagcagca aatagtgtga ttgcttttgc cgcattaggt ggttctgcgt tttatgcttg
      181 caaagtagcc aaagacgata atggcgattt ttatttacac gatttacgtg ctgcgggtgt
      241 cagtgcgccc catcatgctc aagtagagca aggtattaca ggtacttgcg tggtaatggt
      301 gacacccgac tccgaacgta ccatgcacac gtttttaggt attaccgccg aattaagtcc
      361 tcaagaactg gatttggacg cacttaacaa agctcgttat ttatatattg aaggttattt
      421 ggctacatca ccctcagcac gtcatgcagt tgcctctgcc agagccttag ccaaagccca
      481 aaatattaaa accgcactca ccttttctga tcctgccatg gtgcaatatg ctcgtcatgg
      541 tttaaccgaa atgctcgaag acggtgttga tattttattt tgcaatgaac acgaagccaa
      601 attgtgggca aatactgatg acttagcaca ggcaatacaa gctttacatc aattaagtcc
      661 gttattggcg attacctgtg gtgataaagg tgcaattatc agcaacaaag ggcaacaaat
      721 aactattgct ccttttccag tcactgcgct agactcaaac ggtgcaggtg atgcttttgc
      781 aggggcgttt ttgtatggtg taagccaaca atggcctttg gctgattgtg gtcgtttggc
      841 ctcacgtatt gccgcacaag tggttagcca atttggccca cgtttacaaa ctgtgcaata
      901 tagcaagtta ttacaagaaa ctatcacaca acaataacaa tactatgctt agattatgtt
      961 tactggctga gattcccgac caagatgcgc gtggttttcg taccccacgc ggtgaagtgg
     1021 tggtggtgcg acaagggcta gtcgtttatg gttatctgaa tcgttgccca catattggca
     1081 ttagtttaaa ttttcaacct gatgtcttta tggatcttac tcagcggtac ttactatgtg
     1141 ctaatcatgg tgcattgttt cgtgttgaag atggtttttg ttttagaggg ccgtgtcatg
     1201 gtcagtcctt agtgaccatg caactgcaaa ttgaacaagg tgtcatctat ttagttgact
     1261 aaaagttttc atacttaacc ttgttttttt gcaaatcgcc ccctaattgt tgcatggcta
     1321 aagatagtct ctattcttaa aaatgaaata gattattgat agcttgtagg atttacgccg
     1381 tcagtgccat gcgtaaattt gagattaatt agcccattta aggagcaata aaatggcaac
     1441 tgctattaaa acccccaaaa gtaacttagt ttatggtttt gc
//
LOCUS       DAOQMB010000577         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_69102_length_1481_cov_18.051893, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000577 DAOQMB010000000
VERSION     DAOQMB010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69102_length_1481_cov_18.051893"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="lpoB"
                     /locus_tag="PLY05_12220"
     CDS             complement(<1..527)
                     /gene="lpoB"
                     /locus_tag="PLY05_12220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HNC04105.1"
                     /translation="MKTVSILAAAVFLSLSGCASVKYGDATATETVNERFGSTDLQMT
                     AKNMTESLLASPVIGEATAANKRPVMFVERIANKTDEHIDTESVLDSMTTKLLQSGKV
                     RLVDMSRVESARSQLKFQNSDELSNPKTAIAFGQMIGAEFMLYGNLSSIYKEAGSTGN
                     PVDKFTKTWDVYYKFT"
     gene            complement(537..>1481)
                     /locus_tag="PLY05_12225"
     CDS             complement(537..>1481)
                     /locus_tag="PLY05_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04106.1"
                     /translation="YIALNKLEDAQVEMRRAQFLQDQAQQQEPTDNNSLSQEALSEYQ
                     QRLSNTEKLAQRVTSSRQNAAPLYLAGLLYEAKRKFDDALIDYKRALSLVPNNRFLQE
                     DVIRLARQLNRKDEFKHLANVATKLAKNNEGTIVIFYEEDFAPAKEELFLPFPWPEAW
                     YTVAFPYYGDSWHSPQPLTIQHTFLKDSLSSQVLTDTQALAARALKDNYVSLLIRQTL
                     RSQTKHKLQQQAQDQGGAVLGLLVGAYNFLSENADLRSWLTLPRFVHLARFNLPEGEQ
                     SLKLNDQQTVKLKVNSQQIHLVYVVKANNQYYVANWPL"
BASE COUNT          413 a          289 c          299 g          480 t
ORIGIN      
        1 gtaaatttat aataaacatc ccaagttttg gtaaatttat cgacaggatt gcctgtgcta
       61 ccagcctctt tataaatgct gcttaaattg ccatataaca taaactcagc accaatcatt
      121 tggccaaaag caattgcggt tttgggatta ctgagttcgt cactgttttg gaattttaat
      181 tgactacgtg ccgattcaac ccgtgacata tccactaaac gaactttacc cgactgtaaa
      241 agtttggtgg tcatgctgtc taacaccgat tcggtatcaa tatgttcatc ggttttgttg
      301 gcaattcttt ccacaaacat cacaggccgt ttattggctg ctgtggcttc accaatgaca
      361 ggcgaggcta ataacgattc ggtcatattt ttggcagtca tttgcagatc ggttgagcca
      421 aaacgctcat taaccgtttc ggtggcggtg gcatcaccat atttaacgct agcacagccg
      481 cttaaactta aaaaaaccgc cgctgctaaa atggatacag ttttcattgt tttctcctac
      541 agtggccagt ttgccacata atattgatta ttggctttga caacatacac aaggtgaatt
      601 tgttggctat tcaccttaag tttaactgtt tgttggtcat tcagttttaa gctttgttca
      661 ccttcgggta aattaaatct tgctagatgg acaaaacgag gcaatgtgag ccaactacgc
      721 aagtcagcat tctcactcaa aaaattataa gcaccgacta ataatcccaa aactgcaccg
      781 ccttgatctt gggcttgttg ttgcaatttg tgtttggttt gtgaacgtaa ggtttgacga
      841 atgagtagac tgacataatt gtctttaagc gcacgagccg ccaaagcttg agtatcagtc
      901 agcacttgac tggataaaga atcttttaaa aaagtatgtt gaatcgttaa cggctgtggg
      961 ctatgccatg aatcaccata ataaggaaaa gctacagtgt accaagcttc tggccaagga
     1021 aagggcagaa ataattcttc tttggctggc gcaaaatctt cttcataaaa aataacgatt
     1081 gtgccttcgt tattttttgc gagtttagtg gcgacattgg caagatgttt aaattcatct
     1141 ttacgattta attgccgagc caatcttata acatcttctt gtaaaaaacg attgtttggc
     1201 acaaggctta aggcacgttt gtaatcaatt aatgcatcat caaatttacg cttagcctca
     1261 tataacaatc ctgctaaata taaaggtgcg gcattttgtc gactagacgt gactcgttgg
     1321 gctaattttt cagtattgct taaacgttgt tgatattcgc tgagggcttc ttggcttaat
     1381 gagttgttat ctgttggttc ttgttgttga gcttggtctt gtaaaaactg cgctcggcgc
     1441 atttcgactt gggcatcttc taatttattg agagcaatat a
//
LOCUS       DAOQMB010000578         1481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_69107_length_1481_cov_5.331697, whole genome shotgun sequence.
ACCESSION   DAOQMB010000578 DAOQMB010000000
VERSION     DAOQMB010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69107_length_1481_cov_5.331697"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1481)
                     /locus_tag="PLY05_12230"
     CDS             complement(<1..>1481)
                     /locus_tag="PLY05_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HNC04107.1"
                     /translation="KAIRDRKAPPKPGDDIILKVITDGRFAPIDMRFVDSTLPSDPNS
                     KLNRMLDDLISAYHETANNEYMTSGVVDENKGSSLMLFTNIGLGEQSAKNRGFDMKAW
                     IEKRLIDGGVKREHIAFMRDNKAHSKKEKLFDDMRQGRKRILIGGKDMETGVNAQKRL
                     THLFHLDSVWFPSTVEQREGRIIRQGNQNRTVDIRGYATKGSYDSTMWGTVARKARFI
                     EQAFRGDSSVRSMEDVSEASAFEMAAALASGDERYLKLAGLKGDVERLGRLYSAHIDE
                     QKRFAREKAHLESSNKLSASEIEQINAAIAQRKPIKAGEFLANVGKNTFDNRDEFSKA
                     LFEKFKEVAESYKADEIELGSIAGFPIVYNGVELRGGNFAADLRVDLPIDVEPLAIFP
                     IDPAYSITGIATRAVNQTNALDRELISRKNDIEQNTKDLARIGRRIGASFPEMAELNE
                     KQQALNELEQELNAEGNADDDLSQTDNQDTDNKPKFSQSSTIG"
BASE COUNT          393 a          361 c          266 g          461 t
ORIGIN      
        1 tcgccgatgg tggaggattg gctaaactta ggcttgttat cggtatcttg gttatctgtt
       61 tgagataaat catcatcagc attaccctcg gcattaagct cttgctcaag ctcattcaag
      121 gcttgttgct tttcgtttaa ctccgccatc tcagggaatg atgcgccaat tctacggcca
      181 atacgcgcca agtcttttgt attttgttca atatcatttt ttctgctaat caattcacgg
      241 tctaaggcat tggtttggtt gactgccctc gttgcaatac cagtgatgct ataagcaggg
      301 tcaatcggga atatcgccaa tggctctaca tcaatcggca aatccacacg caaatctgct
      361 gcaaaattac ctcctcttaa ctcaacaccg ttataaacaa tcgggaatcc agcaatgctg
      421 ccaagctcaa tctcatcggc tttgtaggat tctgccacct ccttgaattt ttcaaacaag
      481 gctttactaa actcatcacg attatcaaag gtatttttgc caacattggc taaaaactcc
      541 cctgctttaa tcggcttacg ctgtgcaatc gccgcgttaa tttgctcaat ttcagaagca
      601 ctgagtttgt tgcttgattc aagatgggct ttttctcgtg caaaacgttt ttgttcgtca
      661 atatgagccg aatacaaacg ccccaaacgc tcaacatcac cttttaagcc tgctagttta
      721 aggtaacgct catcaccact ggctagggct gccgccattt caaacgcact ggcttcgctc
      781 acatcttcca tgctacggac actgctatca ccacgaaaag cctgttcaat aaaccgtgct
      841 ttacgagcaa ccgttcccca cattgtcgaa tcgtagctgc ccttagttgc ataaccacga
      901 atatcaactg tcctgttttg attaccttga cgaataatac gtccctcacg ctgttcaaca
      961 gtagatggaa accaaacaga atctaagtga aacaaatgag ttaagcgttt ttgggcatta
     1021 acaccagtct ccatgtcttt accgccaatc aaaatccgtt ttctgccttg tcgcatatca
     1081 tcaaatagct tttctttttt ggagtgcgct ttattgtcac gcataaaagc aatgtgttca
     1141 cgcttaacgc caccatcaat aagacgtttt tcaatccatg ctttcatgtc aaagccacgg
     1201 tttttagccg attgttctcc taatccaata ttggtaaaca acatcaaaga cgaaccttta
     1261 ttttcatcaa ctacgccaga tgtcatgtat tcattgttag ctgtttcgtg atatgcactg
     1321 attaaatcat ccaacattct atttagtttg ctgtttgggt cacttggtaa ggttgaatcg
     1381 acaaagcgca tatcaatagg cgcaaaacgt ccatcagtaa tcacttttaa aataatatcg
     1441 tcacctggtt tgggtggagc ttttctgtca cgaatggctt t
//
LOCUS       DAOQMB010000579         1477 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_69480_length_1477_cov_8.941632, whole genome shotgun sequence.
ACCESSION   DAOQMB010000579 DAOQMB010000000
VERSION     DAOQMB010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69480_length_1477_cov_8.941632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLY05_12235"
     CDS             complement(<1..466)
                     /locus_tag="PLY05_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04108.1"
                     /translation="MKKPIIGFTAAVVALAGLAFIGQSIFVSDKTQDAPFSAMSKQNN
                     ESSIAPTLNTTQAPVNVAAPPTPQLTGTDSPLLPMPESNVDAATSMANAMKNGDPRTP
                     PIGRSEPQEKATEAELADPKLYAQYEARQTMRLYKGYINAADTEIPRLQQDIA"
     gene            complement(483..1430)
                     /locus_tag="PLY05_12240"
     CDS             complement(483..1430)
                     /locus_tag="PLY05_12240"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC04109.1"
                     /translation="MPSKDKLLLEIKLTDAGKIAAQKRILLQYNFSEKHKGTISLWDV
                     YQRQNVQGRNEIHSRNADFTQQQAREIVVHRFMDYWLETQSTLTPQDIKDIKQALTYK
                     SYHDGFATYIGIAADIHDDVQYLKDLAKKVTAAEVKSYYQHHKADFKRIERVKAQHIT
                     VSDEKTANMIIEQLKKGAKFADLAKQYSMAMDKSTGGDLGWIVHNRNNPNWLSTLAFV
                     QPINVPSRPVRTPTGQWEILLVTAKEEGYHPANSETVRYLASQVIARQKMVKEYQNTR
                     KNLIQQADIHISPILKKEMINLEKDVMIAKPSEEDHHHH"
BASE COUNT          399 a          266 c          300 g          512 t
ORIGIN      
        1 tagcaatatc ttgttgtaaa cgcggaattt cggtgtctgc ggcattaata tagcccttgt
       61 acaaacgcat agtttgtcgt gcttcgtatt gggcataaag tttaggatct gccaattccg
      121 cttcagtggc tttttcttgt ggttcagaac ggccaattgg aggagtgcgt ggatcaccat
      181 ttttcatcgc attagccatt gaggttgcgg catcaacatt cgattcaggc ataggcaata
      241 aagggctatc tgtgcctgtc aattgtgggg taggtggcgc agcaacgtta acaggagctt
      301 gagttgtatt taaggttgga gcaatagaag actcattatt ttgtttagac atcgcagaaa
      361 aaggtgcatc ttgtgttttg tctgaaacaa aaatactttg gccaataaat gcaaggccag
      421 ctaaagcaac aacggcagca gtaaagccta taattggttt tttcatactt tacctcaagt
      481 cattaatgat gatgatgatc ttcttcgctg ggtttagcga tcatgacatc tttttctagg
      541 ttaatcattt cttttttaag tataggacta atatgaatat ctgcctgctg aattaaattc
      601 ttgcgggtat tttgatattc tttaaccatt ttttgtcgag caataacctg tgatgctaaa
      661 taacgaacgg tttcactatt agcaggatga taaccttctt ctttagccgt aacgagcaaa
      721 atttcccact ggcctgttgg tgtgcgaaca gggcgagaag gtacattgat tggttgtaca
      781 aaagctaatg tgcttaacca attaggatta ttacggttat gaacaatcca gcccaagtca
      841 cctcctgtac ttttatccat tgccatcgaa tattgtttag ctaaatccgc aaattttgcc
      901 ccttttttta gttgctcaat aatcatgtta gctgtttttt catcgctaac ggtaatgtgt
      961 tgggctttaa ctctctcaat gcgcttaaag tcggctttat ggtgttgata atagctttta
     1021 acttctgcgg ctgtgacttt tttggctaaa tctttaaggt attgcacatc atcatgaata
     1081 tctgcggcaa taccaatata tgtggcaaaa ccatcatgat aactcttata agttaaggct
     1141 tgtttaatgt ctttaatatc ttgaggggtt aaggttgatt gtgtttctaa ccaataatcc
     1201 ataaaacgat gaacaacgat ttcacgagct tgttgttgag taaagtcggc attacggcta
     1261 tgaatttcat tacgcccttg cacattttgc cgttgataga catcccatag gctaatcgtt
     1321 cctttatgtt tttcgctaaa gttatattgt aataagattc gtttttgcgc ggctattttg
     1381 ccagcatcag tcagttttat ttctaataaa agtttgtctt tagagggcaa ctaacagcag
     1441 cttgaaaaag tattaaaacc tacctattta gtgtatt
//
LOCUS       DAOQMB010000580         1470 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_70155_length_1470_cov_12.981625, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000580 DAOQMB010000000
VERSION     DAOQMB010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70155_length_1470_cov_12.981625"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /gene="msrB"
                     /locus_tag="PLY05_12245"
     CDS             <1..291
                     /gene="msrB"
                     /locus_tag="PLY05_12245"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002114184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNC04110.1"
                     /translation="QYWNCHEKGVYLCRCCETALFSSETKYDSGSGWPSFYQALNEQV
                     IAEHRDLTHGMIRTEITCQQCGAHLGHVFPDGPKPTGLRYCVNSASLKLKQN"
     gene            314..793
                     /locus_tag="PLY05_12250"
     CDS             314..793
                     /locus_tag="PLY05_12250"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HNC04111.1"
                     /translation="MPNVYDYSATTISGKEVSIADYKGKVLLVVNTASKCGLTPQFKD
                     LEALYEKYKDQGLMILGFPCNQFLSQDPASNEEISEFCQLNYGVTFPMFAKIDVNGDE
                     AHPLFKHLKEAAPGLLGMNAIKWNFTKFLVDRQGNVIERYAPTTVPLDIAKDIEKLL"
     gene            complement(863..>1470)
                     /locus_tag="PLY05_12255"
     CDS             complement(863..>1470)
                     /locus_tag="PLY05_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004747829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanone monooxygenase"
                     /protein_id="HNC04112.1"
                     /translation="RLIPHGLVKVEGNTVIAANGQRHEVDVIIWGTGFEVSHPPIGKK
                     IHNANGQRLSDLWKNSSPEAYLGTSLEDVPNAFLMLGPNVLVYDSFIGLAEAQLDYIV
                     DGLQQVKAQGISKFTIKPSVLRRHNEAVQKHLQTTVFNSGGCKSYYLDANGRNFAAWP
                     WSLATLKQRLSSLKLPEYDLSYAPNVSKAPKGKTKQKAAIA"
BASE COUNT          466 a          271 c          285 g          448 t
ORIGIN      
        1 caatattgga attgtcacga aaaaggcgta tatctatgcc gttgctgcga aacagcttta
       61 tttagttcag agactaagta tgattcgggt tcgggatggc caagttttta tcaagccctt
      121 aatgagcaag tcattgcaga acatcgtgat ttaactcatg gcatgattcg caccgaaatt
      181 acttgtcagc agtgtggcgc acatttaggt cacgtttttc ctgatggccc aaaaccaaca
      241 ggcttgcgtt attgtgttaa ttcggcttca ttaaagctta aacaaaatta aatacatcaa
      301 ctggagagat aaaatgccca atgtatatga ttattctgca accactattt ctggcaaaga
      361 agtgtcgatt gccgattata aaggcaaagt tttattggtg gtgaatacag ctagtaaatg
      421 tggtttaaca ccacaattta aagatttaga agctttgtat gaaaaatata aagatcaagg
      481 tttgatgatt ttaggttttc cctgtaatca atttttaagc caagacccag ccagtaatga
      541 agaaattagt gagttttgcc aattaaatta tggtgttaca tttccaatgt tcgccaaaat
      601 tgatgtcaat ggcgatgaag ctcatccttt attcaaacac ttaaaagaag ctgcacctgg
      661 tttattgggt atgaatgcta ttaaatggaa ctttactaaa tttttagttg atcgtcaggg
      721 caatgtgatt gaacgttatg caccaaccac cgtaccatta gatattgcta aagatattga
      781 gaagttattg taagaaaacg atcatcaata gccttaccac aaacgaataa aacttgatcg
      841 tttgtggtta gcgaatcaga acctaagcta tagctgcttt ttgcttagtt ttgcctttag
      901 gtgctttact gacattaggc gcataactca aatcatactc aggcaatttt aagctgctta
      961 aacgttgttt aagggtggct aatgaccaag gccatgccgc gaagttacga ccattggcat
     1021 ctaagtaata actcttacag ccaccactat taaaaacggt agtttgtaaa tgcttttgta
     1081 cggcttcgtt atgacgacgt aaaacgcttg gtttgatggt aaatttgcta atgccttgag
     1141 ctttaacttg ttgtaaacca tcaacaatat aatcaagttg tgcttcggct aaaccaataa
     1201 atgaatcata aaccaacaca ttagggccaa gcattaaaaa ggcattgggg acatcttcta
     1261 agctagtacc caaataggct tcgggtgaac tatttttcca taaatcagat aaacgttggc
     1321 cattagcatt gtgaattttt ttgccaatgg gaggatgaga tacctcaaaa cctgtgcccc
     1381 aaataatgac gtcaacttca tgacgttgac cattcgccgc aatcacagta ttgccttcaa
     1441 ccttcactaa accatgcgga attaaacggg
//
LOCUS       DAOQMB010000581         1461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_71008_length_1461_cov_11.741821, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000581 DAOQMB010000000
VERSION     DAOQMB010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71008_length_1461_cov_11.741821"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(317..751)
                     /locus_tag="PLY05_12260"
                     /pseudo
     CDS             complement(317..751)
                     /locus_tag="PLY05_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004680746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
     gene            complement(769..>1461)
                     /locus_tag="PLY05_12265"
     CDS             complement(769..>1461)
                     /locus_tag="PLY05_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04113.1"
                     /translation="IYPHYKKEPVVNQLLLESLVKLQAWQDCLEWLPILAKAPIAETI
                     TNQALMKTAYQHSLEVLAKTTGRVDKTITFRQLQEFWQQIPRQHQRDTDVVMSYVQAL
                     ISVGFYDEASVIIARVLNENWHHGLAELYGRVLHAEPELALEQAKTWLKLHPQSPVLL
                     LTLGRLSMQCRDWPMAKVYFEQSLVLHKNTETYAEFVRLLQHLNDPEAGHYLIAGLNQ
                     LISKPLPNLPLP"
BASE COUNT          431 a          281 c          236 g          513 t
ORIGIN      
        1 agattgcaca ctaaattagc aagcttttgt tactatttaa tgtacattta acaggaagtt
       61 tttggtggtt tatttgttaa cgatggcttt ttttacacta aattctcttt aatttattgc
      121 aattttattt attgattatt tagccttttt tcaaggcgca tttaaactaa aacgctatat
      181 ttgcctaata attaggcaaa tattattatt tatcaggcga tatttggctt tatttatcgc
      241 gtaaatgatt cagttttgat aaagccttac ctaataatgt ctctggctgt ttatgtggat
      301 taagtgcatc tacctctaat tcattaccca ctgtttgctc taagttgata atagtttgtc
      361 taaattcttc aggtaaagtc accttttgac catctttatc aaccataaca acaatagctt
      421 ctcctgtggc tacaatactt ttatgttgtt gactaaaaat agcgtactcc attctaaaac
      481 cagagcctct taattcaaca acacgtgcac ctaattctaa ttcatcagga taactgaccg
      541 actttaagta gcggcaacta ttggctgcaa cgacaggatt catttgttgt aaatgatcta
      601 aagcccctat catttgcaaa taactaatcc gtacactttc aaaatagcgg taatatacaa
      661 cattattgac gtgaccaaag gcgtccattt ccccccatgc tacactttga ggctgaacaa
      721 taggaaactc ttttaaactg atttggctca tgttagttat ttatcctatt agggtaaagg
      781 caaattaggc aatggtttgc tgattagctg atttaatcct gctattaaat aatgtcctgc
      841 ttctggatcg tttaaatgct gtaataatcg cacaaattca gcataggttt cagtattttt
      901 gtgcagtacc aaactttgct caaaatagac tttggccatt ggccaatctc ggcattgcat
      961 acttaaacgg cctaaggtta ataataaaac tggactttgt ggatggagtt tcaaccatgt
     1021 tttagcttgt tctaaggcta actctggttc tgcatgaagc actcgcccat acaattcagc
     1081 caaaccatga tgccaattct catttaaaac acgggcaata ataacacttg cctcatcata
     1141 gaaaccgaca ctgattaatg cttgtacata actcatcacc acatctgtat cacgttgatg
     1201 ttggcgaggt atttgttgcc aaaactcttg taattgccta aaggtaatcg ttttatctac
     1261 acgtccagtt gtttttgcca atacttctaa actatgttga tatgcggttt tcatgagtgc
     1321 ttgattggta attgtttctg cgataggggc tttagccaaa attggcaacc actctaaaca
     1381 atcttgccaa gcttgtaatt taaccaagct ctctaataat aattgattaa caaccggctc
     1441 ttttttgtaa tgtggataaa t
//
LOCUS       DAOQMB010000582         1458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_71295_length_1458_cov_10.121169, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000582 DAOQMB010000000
VERSION     DAOQMB010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71295_length_1458_cov_10.121169"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1458)
                     /locus_tag="PLY05_12270"
     CDS             complement(<1..>1458)
                     /locus_tag="PLY05_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNC04114.1"
                     /translation="ACYRCVRYYKDYAGGEDLGVYSAHDNVYFGRPESGTLQSEFSGN
                     LVEVCPTGVFTDKTHSERYNRKWDMQFAPSVCQQCSVGCNTSPGERYGELRRIENRYN
                     GAVNHYFLCDRGRFGYGYVNRDDRPRQPLIRQAEQLTITTVDNALDSAIAMVYGKKVL
                     GIGSPRASLETNFALRELVGAANFATGMTAAEQARVALVAQILQTEGIDTPSLRAIED
                     KDAVLVLGEDVTQTAPRIALALRQSIKNKAEQLAAQRKTSDWHQLAVKNIAQHELSPL
                     YITSLSGTRLDDVAQETCFAAIPDQARLGFAVAHAIDSNAPAVPNMSVEALAWAKKIA
                     EDLLAADQPLVVAGTSAASEDLLLAASNIVQALLVKGKKAAISLVVPEVNSLGVSLLG
                     GQDLDTALASLKAGHYDALVIAENDLYRRLPAGVVDAALQATKQVIVLDHQQTATTDK
                     AHIVLSAASFAEGDGTVVNNEGRAQRFFQVYEASY"
BASE COUNT          390 a          370 c          319 g          379 t
ORIGIN      
        1 aataagaggc ttcgtaaact tggaagaaac gctgagcgcg gccttcgttg ttaactactg
       61 tgccatcacc ttcggcaaaa ctggcagcag acaacacaat atgtgctttg tcagtagttg
      121 cagtttgttg atggtctaac acaatcactt gtttggtggc ttgtaaggcc gcatcaacca
      181 cacccgcagg taaacgacga tataaatcgt tttcggcaat caccagcgca tcataatgac
      241 ctgcttttaa gctcgccaac gcggtatcta aatcttggcc acccaataag ctaacaccca
      301 agctgttcac ttcgggaacc actaagctaa tggccgcttt tttgcctttg actaatagag
      361 cttgaacaat gttacttgcc gctaataata agtcttcact agctgccgag gtgcctgcca
      421 ccactaaagg ttggtcggcg gctaataaat cttcagcaat ctttttcgcc caagctaagg
      481 cttcaacact catattcggg acggcaggtg cattgctatc aatcgcatgc gccacggcaa
      541 aacccaaacg tgcttggtca ggaatcgccg caaaacaggt ttcttgcgcc acatcatcta
      601 aacgtgtgcc gcttaaactg gtgatataca gcggcgacaa ctcatgctga gcaatgtttt
      661 tgaccgctaa ttgatgccaa tcactggttt tacgctgggc agctaattgt tctgctttgt
      721 ttttgatact ttgacgtaaa gctaaagcaa tacgtggtgc ggtttgggtg acatcttcgc
      781 ccaatactaa caccgcatct ttatcttcaa tcgcacgtaa agacggtgta tcaatacctt
      841 cggtttgtaa aatctgtgcg actaaagcca cacgcgcttg ttcagccgcg gtcatgcctg
      901 tcgcaaagtt ggcagcaccg accaactcac gtaaggcaaa gttggtttcg aggctggcgc
      961 gtggtgagcc aatacctaag acttttttgc catacaccat cgcaatagca ctgtctaagg
     1021 cattatcaac cgtcgtaatt gttaattgct cagcttgacg aatcaaaggc tgacgcggac
     1081 ggtcgtcgcg attgacataa ccgtaaccaa aacggccacg gtcacacaag aaataatggt
     1141 ttaccgcacc gttataacgg ttttcgatac ggcgtaattc gccataacgc tcaccgggac
     1201 tggtattaca accgactgaa cattgttgac acacgctcgg tgcaaactgc atatcccatt
     1261 tacggttata acgctcagaa tgggttttgt cggtaaatac ccctgtgggg caaacttcga
     1321 ctaagttacc cgaaaattca ctttgtaagg taccgctttc tggacgacca aagtaaacgt
     1381 tgtcatgtgc gctatacacg cctaagtctt cgccacctgc gtagtctttg taataacgca
     1441 cacaacgata acaggcga
//
LOCUS       DAOQMB010000583         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_71483_length_1456_cov_17.714490, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000583 DAOQMB010000000
VERSION     DAOQMB010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71483_length_1456_cov_17.714490"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLY05_12275"
     CDS             <1..813
                     /locus_tag="PLY05_12275"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC04115.1"
                     /translation="SPQSAIALETEYRLAHLTINVASEASPKQWAEAKNKAEKALKEL
                     QTGKPFFTVVTEYSNADDALQGGDLGWRKSAQLPSLFTDVADKLQRSQFSDIIQSPAG
                     YHIVTVIDKRGAQQMVIKQRHVRHILIKPSEILSLEDAKQKADDLRERLVKGGDFAEL
                     AKTYSEDAGSARNGGDLTWVGAGEMVPSFEEMMFKTAIKDISPVFESQFGWHILQVLD
                     ERDQDFSNQYKRNLARQALYSRQFEEEKSAWLRELRSEAFVQIKGQSESNTP"
     gene            810..>1456
                     /locus_tag="PLY05_12280"
     CDS             810..>1456
                     /locus_tag="PLY05_12280"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNC04116.1"
                     /translation="MKPLLITSGEPAGIGVDLCIQLAQLGLDIPFVVLADPTLLMQRA
                     LKLGVELDIDYYEFGDEWTAPAKGCLKVWPIPLRDRVITGKLNPTNSDYVLEMLERAA
                     NACLDGVAAGMVTAPVQKSVINEAGIAFSGHTEFLQELCGVDKVVMMLACPDLKVALA
                     TTHLPLRQVPDAITSQLLKQVIRITHQDLQRHFAIQSPRLLVTGLNPHAGENGYLG"
BASE COUNT          432 a          313 c          311 g          400 t
ORIGIN      
        1 tctccgcaaa gtgcgattgc tctcgaaacc gaataccgtt tggcacattt aaccatcaat
       61 gtggcaagcg aagcctcacc taaacaatgg gcagaagcca aaaacaaagc cgaaaaagcc
      121 ttaaaagaac tccaaacagg caagcccttt tttaccgttg tcaccgaata ttctaatgcc
      181 gatgatgctc tacaaggtgg tgatttaggc tggcgtaaaa gcgcgcaact tccctctttg
      241 tttacagatg ttgcggacaa actacaacgt agccaatttt cagacatcat ccaaagccct
      301 gctggttatc acattgtcac cgtcattgat aaacgtggcg cacaacaaat ggtcatcaaa
      361 cagcgtcatg tgcgtcatat tctgattaag ccttcagaaa ttttgagctt agaagatgct
      421 aaacaaaaag cagatgattt acgcgaacgc ttagttaaag gtggtgattt tgccgagctt
      481 gctaaaacct attctgaaga tgcaggttct gcacgtaatg gtggtgactt aacgtgggtc
      541 ggagctggcg aaatggtgcc tagctttgaa gaaatgatgt tcaaaaccgc cattaaggac
      601 attagccctg tattcgaaag tcaatttggc tggcatattt tgcaagtttt agatgaacgt
      661 gaccaagact ttagcaatca atataaacgt aatttagctc gccaagcctt atattcacgt
      721 caatttgaag aagaaaaaag tgcatggcta cgtgaattac gttccgaagc ttttgtgcaa
      781 atcaaaggtc aaagcgagag caatacacca tgaaaccatt attaatcaca tcaggtgagc
      841 ctgcgggcat tggtgttgat ttatgtattc aattagcaca actggggcta gatattcctt
      901 ttgttgtttt agctgaccct acgttactca tgcaacgcgc actcaaatta ggcgttgaac
      961 tagatattga ctattatgaa tttggtgatg aatggacagc tcctgccaaa ggttgcctga
     1021 aagtctggcc aatcccatta agagaccgtg ttattacggg caagcttaat cctacaaaca
     1081 gtgattatgt gttagaaatg ttagaacgcg ccgctaatgc ctgtttagat ggtgttgctg
     1141 ctggcatggt cactgcacct gtacaaaaat cggtgattaa cgaagcaggt attgctttta
     1201 gcggccatac tgagttttta caagaactct gtggcgtgga taaagtggta atgatgttgg
     1261 cctgtccaga tttaaaagta gccttagcca ccactcacct ccccctacgc caagtacccg
     1321 atgcgattac ctctcaactt ctcaaacaag tcattcgtat tacccatcaa gacctacaac
     1381 gtcattttgc tattcaatca ccacgattat tagtcacagg gttaaatcct catgcgggtg
     1441 aaaatggtta tttagg
//
LOCUS       DAOQMB010000584         1456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_71488_length_1456_cov_7.239115, whole genome shotgun sequence.
ACCESSION   DAOQMB010000584 DAOQMB010000000
VERSION     DAOQMB010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71488_length_1456_cov_7.239115"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PLY05_12285"
     CDS             complement(<1..721)
                     /locus_tag="PLY05_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNC04117.1"
                     /translation="MDMITILLLIAGLVLLTIGADVLVKGASSLAGSLGISPLVIGLT
                     VVAFGTSAPELSVSVSSAFKGSAEIAVGNVVGSNICNVLLILGLSAIAASLVVQKQLV
                     KFDIPLMIYASLIVLLMSIDGQISRIDGLLLFSGVVAYTVFLIKEARREGVAVVEGAD
                     DIEPPQPLWKNAILIIVGLAMLVLGSQWLVDGAVAIAKYFGLSELVIGLTIVAIGTSL
                     PELATSVMASLKGERDIAVGNI"
     gene            838..1443
                     /locus_tag="PLY05_12290"
     CDS             838..1443
                     /locus_tag="PLY05_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HNC04118.1"
                     /translation="MKLISLYNQFHNTLFNYLKHFDGLAPLALRLYLVPIFWMAGFHK
                     YQSFADTVDWFGNPDYGLGLPFPWLMAFLATSAELAGAVLLTLGLATRWISLPLIVTM
                     IVAIVTVHLPNGWQAIADPNAPFANQQVIDSAEKLAKAKEILQEHGNYDWLTSSGNFV
                     VLNNGIEFATTYLIMLICLFFSGGGRYVSVDYWLAKFLQQK"
BASE COUNT          455 a          333 c          237 g          431 t
ORIGIN      
        1 aaatgttacc aacggcaata tctcgttcac cttttaagct cgccataaca ctggttgcta
       61 actcaggcaa cgaagtacca atcgctacaa tagtcaaacc aatcactaat tcactcaaac
      121 caaaatactt ggcaatcgcc acagcaccat caactaacca ttgagagcct aataccaaca
      181 ttgccaaacc aacaataatt aaaatagcat ttttccataa gggttgtggt ggctcaatat
      241 cgtctgcgcc ttcaaccact gcaacacctt ctctccgtgc ttctttaatt aaaaagacgg
      301 tataagccac aacgcctgaa aataataata agccatctat acgactaatc tgaccatcta
      361 tgctcatcaa taacacaatc aaggaagcat aaatcattaa tggaatatca aatttaacta
      421 attgtttttg cactactaaa gaggctgcaa ttgccgataa acctaaaatc aatagcacat
      481 tacaaatatt gctaccgacc acgttaccca cagcaatctc tgccgaacct ttaaaagccg
      541 aagacacgct cactgacagt tctggtgcgc tagtcccaaa agccaccacg gttaaaccaa
      601 tcactaaagg actaatgcct aaagagcctg ccaaacttga tgcgccttta acaagcacat
      661 cagcaccaat cgttaatagc accaaacctg ctattaacaa caatatcgtt atcatatcca
      721 taagttacct cttttcttaa aaactcaaat actcagcact cattaacaaa accgcatttt
      781 catctgtaac aaaatgcctg aatagttatc taatgtaaca ttcagaggac tttacaaatg
      841 aagctaatct ctctttacaa ccaatttcac aatacactgt ttaactactt aaaacatttt
      901 gatggtcttg ccccactcgc tctaagactt tatttagtgc cgattttttg gatggcaggt
      961 tttcataaat accaaagctt tgctgatact gttgattggt ttggtaatcc tgattatggt
     1021 ttaggtttgc cctttccgtg gttaatggca tttttggcga ccagtgccga actggcaggt
     1081 gcagtgttat taacgcttgg tttagcgact cgttggatta gcttgccact cattgtcacc
     1141 atgattgtgg cgattgttac cgtgcatttg cctaatggct ggcaagcgat tgccgaccct
     1201 aatgcgcctt ttgccaatca acaagtgatt gactccgccg aaaaactggc caaagccaaa
     1261 gaaatactgc aagaacatgg caactatgat tggttaacat ccagtggtaa ctttgttgtg
     1321 cttaataatg gcattgagtt tgccactact tatttaatta tgctgatatg tttgtttttt
     1381 agcggtggtg gtcgttatgt cagtgttgat tattggttag ccaaattttt gcaacaaaaa
     1441 taatttattt attttt
//
LOCUS       DAOQMB010000585         1454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_71724_length_1454_cov_2.776984, whole genome shotgun sequence.
ACCESSION   DAOQMB010000585 DAOQMB010000000
VERSION     DAOQMB010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71724_length_1454_cov_2.776984"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLY05_12295"
     CDS             complement(<1..550)
                     /locus_tag="PLY05_12295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HNC04119.1"
                     /translation="MKASILWLLSLLPVLVYATPQAKIINGQAVTDNEVPWQVALISN
                     NQQPYTSYFCSASIVGNKWLVTAAHCAERSNGKPFYAVIGTTDLLNTQKAQIILIKQR
                     LVQPKYNASNFDNDIALLELAQTIDFVTCGQSCKAINWLKAQDEPLYAVIGTKAWVHG
                     WGVIRDCKQDIETCTTEEKAFEA"
     gene            complement(615..>1454)
                     /locus_tag="PLY05_12300"
     CDS             complement(615..>1454)
                     /locus_tag="PLY05_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04120.1"
                     /translation="VTRIIKVNNDLSSSEIIDAGINTYGIALDKYGKIWVTDAYSTAF
                     ATFNPADVVGTRQTFNQEGLGWQDCYAQGMAVDDNDNIFIAGSACQYGGVVGHYKQIF
                     NNGIFSGVKFVANYTVKSGPTGVAVDGKGNVWSSNRDSNSVSRIILADDPANAKVDSF
                     DVGIGPYNYSDMTGRTVRNITNRQGTWEAIFDGGTKDFEWKKLVWTLEKQLPEGTTVT
                     ASVKAANNKVELSKDYNPVESNQTMKDKNGELIKGQFIKVKFTLTSANQTSTPEITGI
                     DLQ"
BASE COUNT          407 a          317 c          274 g          456 t
ORIGIN      
        1 gagcctcaaa agctttttct tcggtagtac aggtttcaat atcttgttta caatcccgaa
       61 taacccccca accatgcacc catgcttttg tacctatcac ggcataaagc ggctcatctt
      121 gtgcttttag ccaattaatg gctttgcaac tttgaccaca agtaacaaaa tcaatggttt
      181 gtgctaattc taataaggca atatcgttgt caaaattgga agcattatat ttaggttgca
      241 caaggcgttg tttaattaaa atgatttgcg ctttttgcgt attaagtaag tcggttgtgc
      301 caataacggc ataaaagggc ttgccattgc tgcgctcggc acagtgtgcg gctgtgacta
      361 gccatttatt acctacaata ctggcagagc aaaaatacga ggtgtaaggt tgttgattgt
      421 tgctaataag cgcaacttgc cacggtactt cgttatctgt aaccgcttgg ccgttgatga
      481 ttttggcttg tggggtggcg taaacaagca caggcaacaa actcaataac cataaaatag
      541 aagctttcat tgagtgatac tcgtataaaa tacaaaaccc gcatcactgc gggctttgtt
      601 ttacaagaaa ggatttattg caaatcaatg cctgtaattt cgggggtgga tgtttgatta
      661 gcagaggtta aagtaaattt tactttaata aactgtcctt taatcaactc cccatttttg
      721 tctttcatcg tttgattgct ttccactggg ttatagtctt tactcaactc cactttgttg
      781 ttagctgctt tgacagaggc agttaccgtt gtcccctcgg gtagttgctt ttcaagtgtc
      841 cataccagtt ttttccattc aaagtctttg gttccgccat caaaaatagc ttcccatgta
      901 ccttgacggt tggtgatatt acgaacagta cggcctgtca tgtcgctgta gttgtaggga
      961 ccaatgccaa catcaaaaga atctactttg gcattggcag ggtcatcggc caaaataatg
     1021 cgagacactg aattgctatc acggttagaa gaccacacat ttcccttacc atctactgca
     1081 acccctgttg ggccactttt aaccgtgtag ttggcaacga atttaacacc agaaaatatg
     1141 ccgttgttaa atatttgctt ataatgccca acaactccac catactggca agcacttcct
     1201 gcaataaaga tattatcgtt atcgtccacc gccatacctt gtgcatagca gtcttgccaa
     1261 ccaagcccct cttgattaaa agtttgacgt gttccaacta catcagcagg attaaacgtt
     1321 gcaaatgctg ttgaataagc atctgtaacc caaatcttgc catatttatc taatgcaata
     1381 ccgtatgtat ttatacctgc atcaataatt tctgacgaac tcaaatcatt attaacctta
     1441 atgattcttg taac
//
LOCUS       DAOQMB010000586         1450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72062_length_1450_cov_12.829391, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000586 DAOQMB010000000
VERSION     DAOQMB010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72062_length_1450_cov_12.829391"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..560)
                     /gene="nusB"
                     /locus_tag="PLY05_12305"
     CDS             complement(99..560)
                     /gene="nusB"
                     /locus_tag="PLY05_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004974349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC04121.1"
                     /translation="MQTHPNKPQAKNTPAARHKARRFALQGLYEWQLSGNQPFEIEAR
                     YRVENAMHKVDLAYFHELLHQVVAKNRELDNAFEPFLDRKMQQLDPVEHNILRIGSYE
                     LIHHIEIPYKIILNEAVELAKDFGATDSYKYVNSILDELAKTVRKAEKQVQ"
     gene            complement(560..1021)
                     /gene="ribE"
                     /locus_tag="PLY05_12310"
     CDS             complement(560..1021)
                     /gene="ribE"
                     /locus_tag="PLY05_12310"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HNC04122.1"
                     /translation="MKTIEGNFQNANGQYAIIVGRFNSFVVEALLDGALDTLKRHGVD
                     ADNITIIRAPGAWELPLVAKKLAEKKQYDAIIALGAVIRGGTPHFDYVAGECAKGLGV
                     VGLDAGMPVVFGVLTTDSIEQAIERSGTKAGNKGADAAMTAIEMVSLLKAI"
     gene            complement(1038..>1450)
                     /locus_tag="PLY05_12315"
     CDS             complement(1038..>1450)
                     /locus_tag="PLY05_12315"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013118.2"
                     /note="bifunctional enzyme DHBP synthase/GTP
                     cyclohydrolase II-like protein; functions in riboflavin
                     synthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNC04123.1"
                     /translation="GSDVHIALIKGDVQRDTPVVRVHAVNPLRDLLHAKYQGRTGWNL
                     QKSLGEIAKHDAGILVLLGQDYQSSEVVDHIKQFFDGRPRANDGAGMYRTIGVGSQIL
                     RDLGVSKMRLLSSPMRFNALSGFGLEIVEYLATE"
BASE COUNT          424 a          335 c          258 g          433 t
ORIGIN      
        1 cttacgcggt tatcgcttat ttgctcacat ttcaatcgtt ttcaacgatt ttatgttcac
       61 aaaacgcgct aatttcggtg aaatgcgtaa gtcctgagtt attggacttg tttttcggct
      121 ttgcgtacgg ttttggccaa ctcatctaaa atactattta catatttata ggagtctgtt
      181 gcaccaaagt ctttagctaa ttcaaccgct tcattcaaaa taattttgta aggaatttcg
      241 atgtgatgaa ttaactcgta gctaccaatg cgcaagatat tatgctcaac tgggtctaat
      301 tgctgcattt tacggtctaa aaatggctca aaggcattat ctaattcccg atttttagca
      361 acaacttgat gcaacaactc gtgaaaataa gctaaatcca ctttgtgcat ggcgttttcg
      421 acccgataac gcgcttcaat ttcaaaaggc tgattacccg acaactgcca ttcatataag
      481 ccttgtaagg caaaacggcg cgccttatga cgcgcagcag gggtattttt ggcttgcggc
      541 ttattaggat gcgtttgcat tatatcgcct ttaacaagga aaccatttcg attgcggtca
      601 ttgctgcatc tgcacctttg ttacctgctt tagtgccaga acgctcaatg gcttgttcga
      661 tgctatctgt tgttaataca ccaaacacca caggcatacc tgcatctaaa ccaacaacac
      721 ctaaaccttt tgcacattca ccagcgacat aatcaaaatg aggtgtgccg ccacgaatta
      781 ccgcaccgag ggcaataatc gcatcgtatt gttttttctc ggctaatttt ttagccacta
      841 aaggtaattc ccatgcacca ggtgcgcgaa taatggtgat attatcagca tcaacaccat
      901 gacgttttaa ggtgtctaaa gcaccatcta ataacgcttc aacgacaaag ctattaaaac
      961 gcccgacaat aatggcatat tggccattgg cattttgaaa attaccttcg atagttttca
     1021 tctcacgctc caaaaagtta ttcagttgcc aaatattcaa cgatttctaa accgaaacca
     1081 gataaggcgt taaagcgcat tggggagctt aataagcgca ttttgcttac ccccaaatca
     1141 cgcaaaattt gtgaaccaac accaatcgtg cgatacatac ctgcgccatc attagcacga
     1201 ggacgaccat caaaaaactg tttaatatgg tcaaccactt cggatgattg ataatcttgg
     1261 cctaataaca ccaaaattcc tgcatcgtgt ttggcaattt cacccaaact tttttgcaaa
     1321 ttccaaccgg tacgtccttg atactttgca tgtaataaat cacgtaatgg atttactgca
     1381 tgaacacgca caacgggcgt atctctttgt acatcacctt taattaaggc aatatgtaca
     1441 tcgctgcctg
//
LOCUS       DAOQMB010000587         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72268_length_1448_cov_12.119885, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000587 DAOQMB010000000
VERSION     DAOQMB010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72268_length_1448_cov_12.119885"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..274)
                     /locus_tag="PLY05_12320"
     CDS             complement(2..274)
                     /locus_tag="PLY05_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HNC04124.1"
                     /translation="MNKPENKQQIELLKRLARVEGQIRGIQKLIQEPDADCEKVLQQM
                     TAARRALDKAFFEMLACVIETNVLDEQQGDAAQRMSEVKELLTRYA"
     gene            complement(321..>1448)
                     /locus_tag="PLY05_12325"
     CDS             complement(321..>1448)
                     /locus_tag="PLY05_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC04125.1"
                     /translation="LSAFYFFYFAVLGGFMPYWSLYLKQLHFDEKAIGQLMAIGMLTR
                     IFAPNFWGYLADSTGKRIELVRLGASMVAVFWAGIFFVGSHFWLMAAVLFAYSFFQNA
                     ILAQFEAVTIGHLQEQRALYGQIRLWGSLGFIATVAGFGYLLDLISIKWLPIMLLLSA
                     IVAALCSYIIPSPPQQVQAKQRKPFLYLLKQPLLWSFFAAHFLLQLSHAPYYSFFSLY
                     LEEHHYSRSTIGLLWSLGVLAEVIAFTQTHRLLAKFSELQLIIACLLLAALRWLVIAV
                     AVDVPWLLWTVQTFHAFSFAVFHAVAMQFVFREFDIGQQGQGQAVYSALWGLGVAIGS
                     WLTGMVWVAWGTTWVYSIAAAVCVLTAVMIMKVWQQKPLQF"
BASE COUNT          490 a          372 c          242 g          344 t
ORIGIN      
        1 ctcacgcata ccgcgtcaat aactctttaa cttcgctcat ccgttgggca gcatcgcctt
       61 gttgctcatc taaaacatta gtttcaatca cacacgccaa catctcaaaa aaagccttat
      121 ctaaagcacg acgcgcagcg gtcatttgtt gcaacacctt ttcgcaatcg gcatcaggct
      181 cttgaattaa tttttgaatg ccacgaattt gtccttcgac acgggcaaga cgttttaata
      241 attcaatttg ttgcttattt tcaggcttat tcatggtaaa ttacccataa tttatatacc
      301 ccccatagta tcacaactaa tcaaaattgc agtggttttt gctgccaaac cttcataatc
      361 atcaccgcag tcaacacaca aaccgcagcg gctatgctat aaacccatgt tgtgccccac
      421 gctacccaaa ccatacctgt caaccatgaa ccaatcgcca ctcccaagcc ccataaggca
      481 ctataaaccg cttgcccttg cccttgttgt ccaatatcaa actcacgaaa gacaaattgc
      541 attgccactg catgaaacac cgcaaaacta aaggcgtgaa aggtttgtac tgtccacaat
      601 aaccacggta catccactgc cacggcaatg accaaccaac gtagtgcagc taataacaaa
      661 caagcaataa tcagttgtaa ttcagaaaac ttggctaata aacgatgtgt ttgggtaaaa
      721 gcaatgactt ccgctaatac gcccaatgac cacaataaac caattgtgct tcggctatag
      781 tggtgttctt ctaagtacaa actaaaaaag ctgtaatatg gcgcatgaga caattgcaat
      841 aaaaagtgag cagcaaaaaa gctccacaac aaaggctgtt ttaacaaata caaaaatggc
      901 ttgcgctgtt tggcctgtac ctgttgtggt ggactgggaa taatataact gcacaacgcc
      961 gcaacaatcg ccgataacaa caacatgatc ggcaaccact taatgctaat caggtctaac
     1021 aaatagccaa aacctgccac cgtggcaata aatcctaatg atccccataa acgaatttga
     1081 ccatataagg cacgttgttc ttgaagatga ccaatcgtca ccgcttcaaa ctgggctaaa
     1141 atggcatttt gaaaaaagct ataggcaaac aatacggctg ccattaacca aaaatgactg
     1201 cccacaaaaa agattcccgc ccaaaacacc gccaccatgc ttgcacccaa acgcaccaat
     1261 tcaatacgct tgcctgtgct gtcggctaaa tagccccaaa aatttggcgc aaaaatgcgg
     1321 gttaacatgc ctattgccat taattgacca atggcttttt catcaaaatg gagctgtttt
     1381 aaatacaaac tccaataagg cataaaaccg ccaagcaccg cgaaatagaa aaaataaaag
     1441 gcggataa
//
LOCUS       DAOQMB010000588         1448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72329_length_1448_cov_2.840632, whole genome shotgun sequence.
ACCESSION   DAOQMB010000588 DAOQMB010000000
VERSION     DAOQMB010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72329_length_1448_cov_2.840632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1448)
                     /locus_tag="PLY05_12330"
     CDS             complement(<1..>1448)
                     /locus_tag="PLY05_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04126.1"
                     /translation="SIISRACALFINCFYDTDFNWLDELTRQHHNQKEQSIYDLAAEI
                     ITSQIFKEVIKARGQSRQTVFNNDAQDSAKIQRPQTLMLKGLDQAQQMPETSRLCQIA
                     TTQLDPKNALKEEHPLSLRHIDPYLGVSSISQLLPKKGSHNAEQVFPETALKGIPNIL
                     APMLLQRWRLACFAETTGTQKQEALDVNSHSDSPRSTTYGIYLHARRVIEAAFWVLFT
                     QNKSVKLVDKDINNDDTPFDLLVKRELKPHHLDLPHYFIQFAEGDKKGHNVFINDPET
                     GWYLAANYSARAFMAQIMPELPSVKYGDGRLDAMWRGRVQAEKDKKPLKGSYDANMIH
                     AFALYLTKIVVELKPNAGKIPLWYPALKELAAELTTKVRGLSDTTSPLQNVGAIELMQ
                     DGSPQSYQVVTPKAINALKGLFIEQPVYFYSRDGQAGEEFRWQRVWPLKGEAWQYIDP
                     YDNVQEELKAAKVHMVRNSDAGSESRSVWRWD"
BASE COUNT          415 a          312 c          247 g          474 t
ORIGIN      
        1 catcccaccg ccatactgag cgggactcac taccagcatc actatttctg accatatgta
       61 cttttgccgc ttttaactct tcttgaacat tgtcatatgg atcaatatac tgccatgcct
      121 caccttttaa tggccaaaca cgttgccaac gaaactcttc accagcttgg ccatcacgag
      181 aataaaaata gactggctgc tcaataaata aaccctttaa agcattaatc gcttttggag
      241 tgactacctg ataagactgt ggactaccat cttgcattag ctcaatggca cccacatttt
      301 gtagtggact tgttgtatca ctcaaccctc taaccttagt cgttaactca gcagctaact
      361 ccttgagagc ggggtaccat aaaggaattt tgccagcatt gggttttaac tctacaacga
      421 tttttgtcaa ataaagcgca aatgcatgaa tcatattggc atcataacta ccttttaatg
      481 gctttttatc tttttccgcc tgaaccctgc ctcgccacat agcatctaat ctaccatctc
      541 catatttgac cgaaggtaac tctggcatta tttgagccat aaatgctcta gctgaataat
      601 ttgctgccaa ataccaccct gtctctgggt cattaatgaa aacattatgg ccttttttat
      661 ctccctcagc gaattgtata aaataatgag gtaaatcaag atgatgtggt tttaactctc
      721 gtttaactaa caaatcaaaa ggtgtatcgt cattattgat atctttatca actagcttta
      781 cgcttttatt ttgagtaaac aatacccaaa atgcggcttc tatgactcgt cgtgcatgta
      841 agtaaatacc ataagttgtc gaccgtggtg agtcactatg actattaaca tctaaggcct
      901 cttgtttttg ggtgcctgtt gtttctgcaa aacaagctaa acgccaacgt tgcaacaaca
      961 taggggcaag aatatttgga atacctttta atgctgtttc aggaaacact tgctctgcgt
     1021 tatgactccc ctttttaggt agtaattggc taatacttga aacacctaaa tatgggtcaa
     1081 tatgacgtaa actaagcggg tgttcctctt ttaatgcatt cttagggtct aattgggtcg
     1141 tagcaatttg gcacaaacga gaagtttctg gcatttgttg tgcttgatct aaccctttta
     1201 gcatcaacgt ttgagggcgt tgtatttttg ccgaatcttg cgcatcgtta ttaaatactg
     1261 tttgacgtga ttgtcctcta gccttaatca cttctttaaa aatttgacta gtaataattt
     1321 cagctgctaa atcatagata ctttgctctt tctgattatg atgttgccta gtcagttcat
     1381 caagccaatt aaaatcagta tcgtaaaagc aattaataaa caatgcacaa gctctagata
     1441 taatactt
//
LOCUS       DAOQMB010000589         1447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72384_length_1447_cov_15.158046, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000589 DAOQMB010000000
VERSION     DAOQMB010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72384_length_1447_cov_15.158046"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1394
                     /locus_tag="PLY05_12335"
     CDS             222..1394
                     /locus_tag="PLY05_12335"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNC04127.1"
                     /translation="MLSPGTPAMQAVWILLGKTRYPCTFYQSSLEQGVQQVVIPFELS
                     AEYMPAAKSLGNSRVSQLMEANVPINAAFDNIITRNPRMKLLKAQAQKLAEFDVPVLI
                     YGETGTGKELFARAIHNASHRANKPFVPVNCGAIPVELVDSVLFGHKKGAFTGALADK
                     SGLFEQAHGGTLFLDEFGELQPSVQVRLLRVLQEGTFIPVGGTSEQKVDVRIITATHR
                     NLIEAVANGIFREDLFYRVAVGVLHLPPLREREGDLSLLTDSLISSLTMSDSILKHKK
                     IYPDAKNIILQHPWRGNVRELQSTLLRAALWCQGETITAEDIRQALFEMPEKNKDLMD
                     VNISQGIDIQNIISDLVVRYIRKALEHSGQNKTRAAQLLGLKNYQTLNNWIEKYGI"
BASE COUNT          453 a          255 c          299 g          440 t
ORIGIN      
        1 ttgatggcat catgaaaatc taaattcccc tgacacagaa taatgaatac cctccaaaag
       61 aacaagtcac gccctatcta gcttggttaa aacaggaaat tgatatacca attagagcat
      121 accctattga gctatcttct cctgttaact ttggtgaaat ataccttcag gctaatcagc
      181 atttatcaag gattacttta gaggataaag aattatctgt tttgttaagc ccaggtacac
      241 ctgctatgca agccgtatgg attttattag gtaaaactcg ctacccatgc acgttttatc
      301 agtcttcttt ggagcaaggc gttcagcaag ttgtgattcc ttttgaattg tcggcagaat
      361 atatgcctgc tgctaaaagc cttggcaatt caagagttag tcaacttatg gaagcgaacg
      421 ttccgatcaa tgcagctttt gacaatatta ttacgcgtaa tcctcgcatg aagttattaa
      481 aagctcaggc tcaaaaacta gcagagtttg atgtacctgt attgatttat ggagagacgg
      541 gtacaggtaa agagcttttt gctcgagcca ttcataatgc tagccataga gccaataagc
      601 catttgtacc tgttaattgc ggtgctattc ctgtcgagtt ggttgactct gtacttttcg
      661 gtcataaaaa aggtgcattt actggcgcat tagcggataa atcaggacta ttcgagcaag
      721 ctcatggcgg cactttattt ttagatgaat ttggcgaatt acaacccagt gtacaagtta
      781 gattactcag agttctacaa gaagggacat ttattcctgt aggtggtact agtgagcaaa
      841 aagtagatgt ccgtattatt acagcgacac atcgtaatct gatagaggct gttgccaatg
      901 gtatttttag ggaagatttg ttttatcgag tcgccgtagg tgtcttgcat ttgcctccct
      961 taagagagcg tgaaggagac ttatcgctat tgacggatag cttaattagt agtttaacga
     1021 tgagtgattc tatactaaaa cataaaaaaa tttatcctga cgcaaaaaat attatccttc
     1081 aacacccttg gcgaggaaat gtacgtgagt tacagtctac cttgttaaga gcagcattgt
     1141 ggtgtcaagg tgagacgatt acggctgaag atatccgtca ggctttattt gaaatgccag
     1201 agaaaaataa agatttaatg gatgtaaata tctctcaagg tattgatata caaaatataa
     1261 tttctgatct tgttgtaaga tacattcgta aagctttaga gcatagtggt caaaataaga
     1321 cccgtgcagc tcaactttta ggcttaaaaa attatcagac gttaaacaat tggatagaaa
     1381 aatacggtat ttaatcgtaa taaaataaac ttggcacgcc attcgctata tggctggtat
     1441 gaacaga
//
LOCUS       DAOQMB010000590         1445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72573_length_1445_cov_15.005755, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000590 DAOQMB010000000
VERSION     DAOQMB010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72573_length_1445_cov_15.005755"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            361..>1445
                     /locus_tag="PLY05_12340"
     CDS             361..>1445
                     /locus_tag="PLY05_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNC04128.1"
                     /translation="MAQYKAPLRDMQFVLHDVLNVEQHYASIPVFAETNRELVDSILE
                     AGAQFCENELSPINRSGDEEGCQFNDGEVTTPKGFKEAYKKFVELGFGALSAPVEHGG
                     QGLPPSLGVVMSEMMGTSNWSWGMYPGLSHGAINTLEAHGDAHQKEVYLTKLISGEWT
                     GTMCLTESHAGSDLGIIRTKAEPQADGSYKITGTKIFISAGEHDMADNIIHIVLARLP
                     DAPKGTKGISLFIVPKFLPTADGGVGERNGVKCGSIEHKMGIKASATCVMNFDGATGY
                     LIGPPNKGLNCMFTFMNTARIGTAIQGVAAAEGSFQGALEYARDRTAMRSLTGAKNPE
                     KEADLIIVHPAVRQLLLTQKVFAEGGRA"
BASE COUNT          425 a          256 c          303 g          461 t
ORIGIN      
        1 cttaaagcaa gaaaaaaagc agtttttttc acaaaattga ctcctcattt ttagcggtca
       61 ttttagcaga gttaaagcga ctaagtatgg atgttttgtt ttagtctaga aactgttaca
      121 catttgcgta cgaattcgat ttaatgtttg tttaattttt tgttgaatcc ttgtattaga
      181 caaaaaaatg agaatttgag ttaaaaatat gatcaatgtt aacactgtga acgtgcagtc
      241 attaaattga ctgtattgtc gagacatgaa tttttaaact atatcaatgt cagtctgtcc
      301 agattttatc tgtggctttt ttggataaac gtcactctat tttgacgaga ggttgttaca
      361 atggctcaat ataaagctcc actccgcgat atgcaatttg ttttacatga tgtgttaaat
      421 gttgaacaac attatgcaag cattccagtt tttgctgaaa ctaaccgtga attggttgac
      481 agtattttag aagccggtgc tcaattttgt gaaaacgaac tttctccaat caatcgtagt
      541 ggtgacgaag aaggttgtca atttaacgat ggcgaagtaa caacaccaaa aggctttaaa
      601 gaagcataca aaaaatttgt tgagcttggc tttggcgcat tatctgctcc tgtcgagcat
      661 ggcggtcaag gtttaccacc ttcattaggt gtagtaatga gcgaaatgat gggtacatcc
      721 aactggtctt ggggtatgta tcctggtttg tctcacggtg caatcaatac tttagaagca
      781 cacggtgatg cacatcaaaa agaagtttac ttaaccaaat taatttctgg tgagtggact
      841 ggtactatgt gtttaacaga atctcatgca ggttctgact taggtatcat tcgtactaaa
      901 gctgaaccac aagctgatgg cagctacaaa atcactggta ctaaaatctt catttctgct
      961 ggcgaacacg acatggctga taacatcatc catatcgttt tagctcgttt accagatgca
     1021 ccaaaaggta ctaaaggtat ttctttgttt atcgtgccaa aatttttacc aactgccgat
     1081 ggtggtgttg gtgagcgtaa tggtgttaag tgtggttcta tcgaacacaa aatgggtatc
     1141 aaagcctctg caacttgtgt gatgaacttt gatggtgcaa ctggttactt aatcggccca
     1201 ccaaataaag gcttaaactg catgttcacc tttatgaaca ctgcacgtat tggtacggct
     1261 attcaaggtg ttgctgctgc tgaaggttct ttccaaggtg ctttagagta tgcgcgtgac
     1321 cgtaccgcaa tgcgttcttt aacgggtgca aaaaacccag aaaaagaagc tgacttaatt
     1381 atcgttcatc cagccgtacg tcaattatta ttgactcaaa aagtatttgc tgaaggtggc
     1441 cgtgc
//
LOCUS       DAOQMB010000591         1441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_72954_length_1441_cov_20.209235, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000591 DAOQMB010000000
VERSION     DAOQMB010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72954_length_1441_cov_20.209235"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..600)
                     /locus_tag="PLY05_12345"
     CDS             complement(193..600)
                     /locus_tag="PLY05_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000993197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04129.1"
                     /translation="MLAIQEITQHINALRAQVPLSVIDNEQEYDNAVNALNELLDAGG
                     ADEHHPLAVLVTLLSDFIADYEHKHTPRPQVTGRAMLAFLMEQHGLKQGDLSEIGSQG
                     VVSELLSGKRELTTNHIKLLASRFNVPVSVFLG"
     gene            complement(603..884)
                     /locus_tag="PLY05_12350"
     CDS             complement(603..884)
                     /locus_tag="PLY05_12350"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNC04130.1"
                     /translation="MRVISNKALKDFAEIHTQADSLLQSWRQLIESRDFGSFADLKQV
                     FNSVDRVGDFYVFNIGGNKYRLIAAIHFDKQRLFIRHVFTHTQYNHWRP"
     gene            complement(999..>1441)
                     /locus_tag="PLY05_12355"
     CDS             complement(999..>1441)
                     /locus_tag="PLY05_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04131.1"
                     /translation="RPQIEEVKPYTQDELNRLLNQAAKPLYHHLIDCQQCSLEDTRYC
                     VEGFGLGNTFDCLLLCFDDAADKRYKLMNQVIKARVSGRSVFVPFNSSDALAQPTNAP
                     ITRKWGNTKEYEAFINHWTGCKFCKPNLNRYCDEGQRLEKEANC"
BASE COUNT          419 a          318 c          266 g          438 t
ORIGIN      
        1 gggggtaacg ctaaaataat taccgtcacc taccgttgcc ttttcgtccg attttgttgc
       61 taccgtccga ttaccgttgc gttttaaata acaactagct gcccctttaa atagtggtgc
      121 agatagttaa gccataaatc acaaaaccgc cataaggcgg cttgtggtgc taggtatatg
      181 tgctactagc aattaaccta aaaagactga tacaggcaca ttaaagcgag atgctaataa
      241 cttaatatga ttagtagtca actcacgttt accacttaat aattctgata cgacaccttg
      301 tgagccaatt tcggataaat ccccttgttt aagtccgtgt tgctccatta aaaaagccaa
      361 catagcacgg cctgtgactt gggggcgtgg tgtgtgttta tgctcatagt cggcaataaa
      421 atcacttaac aaggtcacta ataccgctaa agggtgatgt tcgtctgctc cccctgcatc
      481 aagcaattca tttaaagcat taacggcatt atcatactct tgctcattgt ctataaccga
      541 caaaggcact tgtgcgcgta gggcgttaat atgctgagta atctcttgaa tggctaacat
      601 aattacggcc tccagtgatt gtattgtgta tgagtaaaaa catggcgaat aaataagcgt
      661 tgtttgtcaa aatgaatggc ggctatgagt ctgtacttgt tgcctccaat gttaaacaca
      721 taaaaatcgc ctacacggtc aacactatta aatacctgtt ttaagtcggc aaaactgcca
      781 aagtcacggc tttctataag ctgtcgccaa gactgcaata agctatccgc ttgtgtatgt
      841 atttcggcaa agtcttttaa tgctttattg ctaatcacgc gcattatgca atcctatgtt
      901 tgtcggtttg aatatatctc attttgagat tacaaccaac aaaaagggca tacctaaaca
      961 gatatgccct taacgccaaa attgacgaac accctaaatt aacaattagc ctccttttct
     1021 aaccgttgcc cctcatcaca atatctattc aaattaggct tacaaaactt acaacctgtc
     1081 cagtgattga taaacgcctc atactccttt gtattgcccc attttcgcgt tataggcgcg
     1141 tttgtaggct gtgctagtgc atcactacta ttaaacggta caaacacgct acggcctgat
     1201 acacgcgctt taataacctg attcattagc ttgtatcttt tgtctgctgc atcatcaaaa
     1261 cacagtaaca aacaatcaaa ggtattacct aaaccaaaac cctctacaca atacctggta
     1321 tcttctaggc tgcattgttg gcaatctatt aagtgatgat atagcggctt tgctgcttgg
     1381 ttgagaagtc tgtttagttc gtcttgtgtg taaggtttaa cttcctcaat ctgaggacgt
     1441 t
//
LOCUS       DAOQMB010000592         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73162_length_1439_cov_9.236994, whole genome shotgun sequence.
ACCESSION   DAOQMB010000592 DAOQMB010000000
VERSION     DAOQMB010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73162_length_1439_cov_9.236994"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..809
                     /gene="gloB"
                     /locus_tag="PLY05_12360"
     CDS             36..809
                     /gene="gloB"
                     /locus_tag="PLY05_12360"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HNC04132.1"
                     /translation="MLKNIAVPMFNDNYLWLLISPQKTVIAVDVGDYALLATYLQAHN
                     LQLTTVFITHHHHDHITGLAELHTHFPDVTIYAAKNINYATQILTGGELLNISDFGRF
                     LVLDVKGHTAIHLAFYHLQQQLLYCGDSLFSGGCGRIFPDGNAENLFDSLQKIALLPN
                     STQVCAAHEYTLDNLKFAQHIEPNNLKLQHYQNICQLRRQQGQATLPCLLATEKEINP
                     FLRCHLLKERMQILTHQTCLTNKSTFCTLRAYKNNWQPN"
     gene            1039..>1439
                     /locus_tag="PLY05_12365"
     CDS             1039..>1439
                     /locus_tag="PLY05_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04133.1"
                     /translation="MQYWLTFRKSAQTAALFALVSLVLTACTAPVKTNQATNPITTKP
                     NVISSFFGEQQTTATKTNLKLTENEEESGYYDTTGVVASQDDIDELLGVTEMEAAVLS
                     PEDLAKFGDVWERTRAGFKLEAIDNERIAAQR"
BASE COUNT          449 a          281 c          252 g          457 t
ORIGIN      
        1 cgggcatcag gttgccaaat atgaggtagt ttcatatgtt aaaaaatatc gctgtgccga
       61 tgtttaacga taattattta tggctattaa tttcaccaca aaaaacggtt atcgctgtag
      121 atgtaggaga ttacgcgcta ttagcgactt atttgcaagc ccacaattta cagttaacga
      181 cagtatttat cactcatcat catcatgacc atattactgg ccttgctgag ttacatacac
      241 attttccaga tgtgaccatt tatgcagcca agaatattaa ctatgccaca caaatattaa
      301 caggcggaga actgctaaat atttcagatt ttggtcgttt tttggtgtta gatgttaagg
      361 ggcatacagc tattcattta gcgttttatc acttacaaca acaattatta tattgtggtg
      421 atagtttatt tagtggtggc tgtggccgta tttttcctga tggtaatgct gaaaatctat
      481 ttgactcttt acaaaaaatt gccttattac ccaatagtac acaggtatgt gctgcacatg
      541 aatatacctt agataattta aaatttgcac aacacattga acctaacaac ctaaaactac
      601 aacattatca aaatatttgc caattacgcc gacaacaagg tcaagcaaca ttgccctgct
      661 tattagccac tgaaaaagaa attaatcctt ttttacgttg ccatctatta aaagaacgca
      721 tgcaaatatt aactcatcaa acttgcttaa ccaacaaatc aacattttgt acgctgcgtg
      781 cttataagaa taattggcag cccaattaaa aacagttgtc aaaatcaaca ttttgttcaa
      841 tatacgttca ttttgccgca cttcgttaca gtttagttgt atttcattat ggtttttctt
      901 gcctgagaag ttaagtctcg taaaattata ggattgcctt ttagattagg cttaccctta
      961 tttattcata agtttactta cgcactcatt tagtatttag cgtagggaat ctatttttta
     1021 atttttggag tatatccgat gcaatattgg ctcactttcc gcaaatctgc ccaaactgcg
     1081 gcattatttg ctctcgttag cctcgtattg accgcctgta ctgcccctgt caaaaccaac
     1141 caagctacaa atcctattac taccaaacct aatgttatct cttctttttt tggtgaacag
     1201 caaacaacag caactaaaac aaacctaaag ctgacagaaa acgaagaaga atcaggctac
     1261 tacgacacga caggtgttgt ggccagccaa gatgatattg atgagttatt aggcgtaacg
     1321 gagatggagg cggcggtcct cagtccagag gatttagcaa aatttggtga tgtgtgggaa
     1381 cgtactcgtg ctggttttaa attagaggct atcgataacg aaagaattgc cgcccaacg
//
LOCUS       DAOQMB010000593         1439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73212_length_1439_cov_3.143786, whole genome shotgun sequence.
ACCESSION   DAOQMB010000593 DAOQMB010000000
VERSION     DAOQMB010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73212_length_1439_cov_3.143786"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="PLY05_12370"
     CDS             <1..230
                     /locus_tag="PLY05_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04134.1"
                     /translation="SNTNINSVVAITGNTSITGNLAVAGAISGTGGSGASIAGNVTVS
                     NGDVVADGISLKTHTHPYTDNGNPMTTGVPS"
     gene            305..>1439
                     /locus_tag="PLY05_12375"
     CDS             305..>1439
                     /locus_tag="PLY05_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04135.1"
                     /translation="MAKSKTTDTEQSSPTWVQWIKNIAIGKEDSPEQGALAHIDLMDV
                     EPTSTAILLGGNNQARSRQQIYAKYQQMLQNSFINAGLRLHVTAALGGHESKGDVVFI
                     ECTPEAEKDPKKKKIIDDLNHDLRELLNKNIYTLAFNAIAWGDSYARIYSQDKVGVTD
                     LLCDEMVLPPLVQPFEQGSKTVGYVVGTATNSSGVKLSTVQMVRVKMPRTIYTPQARV
                     MQKAFKTAILEDDNANLPYLPSLVGGSFLEGIEESFNHLIMSISGMVGQRIQDGIDEA
                     LLTVNMSDMTIEQQRATMDNIKQMFDETAQQTAQAIKEGRSLLGKIRRFIPVSNEKQL
                     VQVQGGTGSMRTASISIEDVMFHAKQLAGGLGIDLSMLGFADLL"
BASE COUNT          461 a          281 c          328 g          369 t
ORIGIN      
        1 caagcaatac caacattaac agcgtagtag cgattacagg caatacgtcc attacaggta
       61 atttagcggt agcaggtgca atcagtggta caggcggtag tggtgcaagc attgcaggta
      121 atgtgacggt atcaaatggc gatgtcgtag cagacggtat tagccttaaa acacatacgc
      181 acccctatac ggataacggc aatccgatga caacaggtgt accgagttaa tctgtaaccc
      241 tctctaattc acccaaaaaa acaagcgaca atagcgttaa tttttaatta ttagcgttat
      301 tgttatggcc aagtctaaaa ccacagatac agagcaaagc agcccaacat gggtgcaatg
      361 gattaaaaat atcgccattg gcaaagaaga ttcacccgaa caaggcgcat tggctcacat
      421 tgatttgatg gacgttgagc caacatcaac cgcgattttg ttaggtggca acaatcaagc
      481 gcgaagccgt caacagattt atgccaagta tcagcaaatg ttgcaaaact catttatcaa
      541 tgctggcttg cgtttgcacg ttaccgcagc tttaggcgga cacgaaagta agggtgatgt
      601 ggtttttatt gaatgtacac ccgaagccga aaaagacccc aaaaagaaaa aaatcattga
      661 tgatttaaac catgatttac gcgaattatt aaacaaaaat atctatacct tagcctttaa
      721 tgcgattgca tggggggata gttatgcgcg tatttacagt caagacaaag taggtgtaac
      781 cgatttactg tgtgatgaaa tggtattgcc gccattggtt caaccctttg agcagggcag
      841 taaaaccgtg ggctatgtgg tgggtactgc tactaattca agcggcgtaa aactctctac
      901 tgtgcaaatg gtgagggtta aaatgccgcg aactatttac acgccacaag cgcgagtgat
      961 gcaaaaagcc tttaaaacag cgattttaga agatgataac gccaatttgc cctatttgcc
     1021 gtcattagtt ggcggaagtt ttttagaggg tattgaggaa tcttttaatc atttaattat
     1081 gtctatctca ggcatggtag gacaacggat tcaagacggt atagacgaag cgttactcac
     1141 cgtaaatatg tcagatatga ctattgagca acaacgcgca acaatggaca acattaaaca
     1201 aatgtttgat gagaccgcac aacagaccgc gcaagcgatt aaagaggggc gttcactgct
     1261 tggtaaaata cgccgattta ttcctgtatc aaacgagaag caattagtcc aagtgcaagg
     1321 cggtacaggc tcaatgcgta cagccagtat cagtattgaa gacgtaatgt tccatgcaaa
     1381 acagttagca ggtggactag gtattgattt atctatgctt ggctttgctg atttattat
//
LOCUS       DAOQMB010000594         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73483_length_1436_cov_11.916003, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000594 DAOQMB010000000
VERSION     DAOQMB010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73483_length_1436_cov_11.916003"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1436
                     /gene="argS"
                     /locus_tag="PLY05_12380"
     CDS             <1..>1436
                     /gene="argS"
                     /locus_tag="PLY05_12380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005403940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNC04136.1"
                     /translation="LQQLQQQQVIPTDWVNKSTLERTRDNSHGDWASNLAMVAAKAAG
                     LKPRDLAQKIVDALPNNAVISKIDIAGPGFINFYLQAQAEFAVLNTILEQKEQFGCSQ
                     YGQQQKVQVEFVSANPTSSLHVGHGRGAAYGMTVANLLEAIGYQVEREYYVNDAGRQM
                     AILATSTFLRYLQLNGAEFKFPSNGYKGDYIFDIAKTVISAHGTQWQQDINAVFADVP
                     ADEVKNEAGEVISGDKEAHIDGLIDNSRRLLGAEGYNVFFRAALDSILDDIKDDLAGF
                     GVTYDQWFSEKTLADDGSIDKVVKRLQDNGFIYEKGGALWFKSTDFGDEKDRVVVRDN
                     GQATYFASDIAYHLNKFERGLDKIVNIWGADHHGYITRVKAAITALGLDANKLDVLLV
                     QFVTLWRSGEQVQMSSRSGQFITLRELREEVGNDAARFYYITRKSEQHIDFDLDLAKS
                     QSKDNPVYYIQYAHARICRVFEKLQEKG"
BASE COUNT          429 a          272 c          307 g          428 t
ORIGIN      
        1 ttacaacagt tacagcaaca gcaagtgatt ccaactgatt gggtgaataa aagcacttta
       61 gaacgtactc gcgacaatag tcatggcgac tgggcaagta acttagccat ggttgccgcc
      121 aaagcagcag gtttaaaacc gcgtgatctg gcacaaaaaa ttgttgatgc tctaccaaac
      181 aatgccgtta ttagcaaaat tgacattgct gggccgggtt ttattaattt ttatttgcaa
      241 gctcaagctg aatttgcggt gttaaatacc attcttgagc aaaaagaaca gtttggttgt
      301 agccaatatg gccaacaaca aaaagtacaa gttgagtttg tctcggctaa tccaacctcc
      361 tcccttcacg tcgggcacgg tcgtggtgct gcttatggca tgaccgttgc caacctttta
      421 gaagcaattg gctatcaagt tgaacgtgaa tattatgtca atgacgctgg tcgtcagatg
      481 gctattttag ccaccagtac cttcttgcgt tatttacagc ttaatggcgc agaatttaaa
      541 tttccatcaa atggttacaa aggcgattat atttttgata ttgccaaaac agtcatttca
      601 gcacatggca cacaatggca acaagacatc aatgctgttt ttgccgatgt acctgctgat
      661 gaagttaaaa acgaggctgg cgaagtcatt agtggtgaca aagaagccca tattgatggc
      721 ttaattgata atagccgtcg tttattaggt gctgaaggct acaacgtatt tttccgtgct
      781 gccttagata gcatcttaga tgatattaaa gacgatttag cgggctttgg cgttacctat
      841 gaccaatggt tctccgagaa aaccttagcg gatgatggct caattgataa agtcgttaaa
      901 cgcttacaag ataatggttt tatttatgag aaaggtggtg ctttatggtt taaatccacc
      961 gactttggtg atgaaaaaga ccgtgttgtt gtccgtgata atggccaagc cacctacttt
     1021 gcttctgata ttgcctatca cttaaacaaa tttgaacgtg gcttagataa aatcgtcaat
     1081 atttggggtg cagatcacca cggctatatt acacgtgtta aagcagcaat tacggcttta
     1141 ggtttagatg ccaataaatt agacgtattg ttagtacaat ttgtcacctt atggcgcagt
     1201 ggtgaacaag tacaaatgtc ctcacgttcg ggacaattta ttactttacg cgaattacgt
     1261 gaagaagtgg gtaatgatgc agctcgcttt tattacatta ctcgcaaaag cgaacaacat
     1321 attgattttg acttagattt agccaaatca caaagcaaag ataaccctgt ttattatatt
     1381 caatatgctc atgcgcgtat ttgccgtgtg tttgaaaaac tgcaagaaaa aggctt
//
LOCUS       DAOQMB010000595         1436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73540_length_1436_cov_2.908762, whole genome shotgun sequence.
ACCESSION   DAOQMB010000595 DAOQMB010000000
VERSION     DAOQMB010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73540_length_1436_cov_2.908762"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1436)
                     /locus_tag="PLY05_12385"
     CDS             complement(<1..>1436)
                     /locus_tag="PLY05_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004906634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC04137.1"
                     /translation="ILNARSAEITQYLDQDLNQKVKQLLAAQQAGNQQQQLNQLVNEL
                     RDEFGKRAFDASGNLIDKEAIDSPLGQRYLALKNSNSQSDSHENQVFSHLLTFFSRYY
                     DNGDFISQRRYKGDTYAIPYSGEEVVLHWANKDQYYTKSGENFANYRFKLSDGRSVLF
                     KLVAADTARENRKDNDKDRRFKLIDADFVQTLVDEDGESYEQALEQIRVVNDELVIHF
                     EYAPVSKGTKQEDLNNQAIATILSHQEVQTHWAELKQKQPTEKNPERTLLQKCLERYT
                     ASNTADYFIHKDLGKFLRGELDFYIKNEVLHLDDVQNANTFASIEQNLRMIQCLRAVA
                     LELVDFLASLEDFQKRLWLKKKFVVAAHYCLTLDRVPRALYAEIAANESQLNQWLSLG
                     CIESKQVGAVKKWLLGEVVEPVKGQQDLLADDAPVQVSPFCFLMVDTALFGVDFKARL
                     LRAVDDLDERVDGVLVHGDNFQGLSLLQ"
BASE COUNT          414 a          377 c          236 g          409 t
ORIGIN      
        1 cctgcaacaa actcaacccc tgaaaattat cgccatgcac caacacccca tccacacgct
       61 catccaaatc atcaaccgcc cgtaacaacc tcgccttaaa atcaacacca aataaggccg
      121 tatccaccat caaaaaacaa aacggactga cttgcacagg cgcatcatcc gccaacaaat
      181 cttgctgccc cttaacaggc tcaaccacct cacccaacaa ccacttttta accgcgccca
      241 cctgcttaga ctcaatacaa cccaaactta accactgatt taactgactc tcattcgccg
      301 caatctcggc atacaacgca cgcggcacac ggtctaaagt taagcaataa tgcgccgcta
      361 ccacaaattt cttttttaac cacaaacgct tttgaaaatc ttccaaactc gccaaaaagt
      421 ccaccaactc caacgccacc gcgcgtaaac actgaatcat gcgcaaattc tgctcaatac
      481 tggcaaaggt attcgcattt tgcacatcat ccaaatgcag cacttcgttt ttaatataaa
      541 aatccaactc accacgcaaa aacttaccca agtctttatg aataaaataa tcggcagtat
      601 tactggcggt ataacgctct aagcattttt gtagcaaggt acgctcagga tttttttcgg
      661 taggctgttt ttgctttaac tctgcccagt gcgtttgcac ttcttgatgc gacaaaatcg
      721 tcgctatggc ttggttattt aaatcttctt gttttgtacc tttgctaaca ggtgcatatt
      781 caaaatgaat cactaattca tcattaacta cgcgtatttg ctcaagtgct tgctcataac
      841 tttcgccatc ttcatccacc agcgtttgca caaaatcggc atcaattaac ttaaaacggc
      901 ggtctttgtc attatctttg cggttttcac gcgccgtatc tgccgcgacc aacttaaaca
      961 atacactacg accatcgctt aatttaaaac ggtaattggc aaagttttcg ccacttttgg
     1021 tgtaatactg gtctttgttt gcccaatgca acaccacttc ttcgccactg taaggaatag
     1081 cgtaagtatc acctttgtaa cggcgttggc taataaaatc accgttatcg taataacggc
     1141 taaagaatgt cagcaaatgg ctaaagactt gattttcgtg gctgtcagat tggctgttgc
     1201 tattttttag ggctaaataa cgttgaccta ggggcgaatc aatggcttct ttgtcgatta
     1261 aattgcctga cgcatcaaag gcacgtttgc caaattcatc gcgtagctca ttaaccaact
     1321 gattaagctg ctgctgttga ttgcctgctt gttgtgcagc taataactgt ttaacttttt
     1381 ggtttaagtc ttggtctaag tattgggtaa tttcggcact acgcgcattc aaaata
//
LOCUS       DAOQMB010000596         1434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73701_length_1434_cov_8.245105, whole genome shotgun sequence.
ACCESSION   DAOQMB010000596 DAOQMB010000000
VERSION     DAOQMB010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73701_length_1434_cov_8.245105"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..>1434
                     /locus_tag="PLY05_12390"
     CDS             12..>1434
                     /locus_tag="PLY05_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HNC04138.1"
                     /translation="MTLLGQAAIFLGAALLVVPLCHRLGLATVLGYLITGILIGPNLL
                     DLSGDAQTVLHFAEFGVVMLLFIIGLELEPSRLWVLKRSIFVLGGLQVFVTGCLFSAI
                     GLALDFSLITSCIAGFGLAMSSTAFVMQLLADKKQLTTRHGREAFAILLFQDMAVIPL
                     LAILPILSGGGTQEYNFTYFAKVIGVFALLILASRVVIRPVFRLIAASKANELLTAAA
                     LFIIIGIAVVMDKLGLSMALGAFVTGVLLADSEYKHELEANIQPFKGLLLGLFFMAVG
                     MSTDMHRLINEPLLIVGGAILLISIKFLVLMLIGKFVGNPWQTSIRLGVSLAQGGEFA
                     FVLFSSATGLKLLPTELSDRLILIVTLSMALTPLAFFLLERFVEPLWNKQEKRAFDEI
                     AADDHENPVVIAGFGRFGQIIARVLKMHKIPFTALEGDARQVDFVRRFGNKIYYGNPS
                     HIEMLRAAKLDKAKVFILAIDDVS"
BASE COUNT          335 a          263 c          324 g          512 t
ORIGIN      
        1 gagcattaag catgacgctt cttggccaag ccgccatttt tttaggtgcg gctctgttag
       61 tcgttcccct ttgccatcgg cttggtttag ccaccgtgtt aggctacctg attacaggca
      121 ttctaattgg ccccaattta ctcgacttat cgggtgatgc acaaacggta ttacattttg
      181 ccgaatttgg tgtggtcatg ttgttgttta ttatcggttt agagttagaa ccgtcacgat
      241 tatgggtgtt aaaacgctcg atttttgtct taggtggttt gcaagttttt gttacaggct
      301 gtttgtttag tgccattggt ttggccttag attttagtct aatcaccagt tgtattgcag
      361 gttttggttt ggccatgtcc tcaaccgctt ttgtcatgca gttattagcc gataaaaaac
      421 aattaacgac tcgtcatggc cgtgaagcct ttgcaatttt gctttttcaa gacatggcgg
      481 tgattccctt attggcgatt ttaccgattt tgtcaggtgg cggcacacaa gaatataatt
      541 ttacctattt tgccaaagtg attggcgtat ttgccttatt aattttggcc agtcgagtgg
      601 tgattcgccc tgtatttaga ttaattgcgg ccagtaaagc caatgagcta ctcaccgctg
      661 ctgccctgtt tattattatt ggtattgctg tcgtcatgga caaattaggg ttgtcgatgg
      721 cattaggtgc atttgtcact ggtgttttgc ttgccgattc cgaatacaaa catgaattag
      781 aagccaatat tcaacccttt aaaggcttgt tattagggct gttttttatg gctgtgggca
      841 tgagtaccga tatgcaccgt ttaattaacg aacccttatt aattgtcggt ggtgcaattt
      901 tattaatcag cattaaattt ttagtcttaa tgttgattgg taaatttgtt ggcaatcctt
      961 ggcaaaccag tattcgtttg ggagtcagtt tggcacaagg gggggaattt gcttttgtct
     1021 tatttagcag tgcgacaggc ttaaaattat tacccaccga actcagtgac cgtttaattt
     1081 taattgtcac tttgtctatg gccttaacac cacttgcctt ctttttgttg gaacgttttg
     1141 ttgagccatt atggaacaaa caagaaaaac gtgcttttga tgaaattgct gctgatgacc
     1201 acgaaaatcc agtggtgatt gcaggttttg gtcgttttgg tcaaattatt gcccgcgtct
     1261 taaaaatgca caaaattcct tttactgctt tagagggtga tgcgcggcaa gttgattttg
     1321 tgcgtcgttt tggtaataaa atttattatg gcaatcctag tcatatcgaa atgttacgcg
     1381 ccgccaaact cgacaaagcc aaagtgttta ttttagcgat tgatgatgtc agtg
//
LOCUS       DAOQMB010000597         1432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_73913_length_1432_cov_56.801743, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000597 DAOQMB010000000
VERSION     DAOQMB010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73913_length_1432_cov_56.801743"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1010)
                     /locus_tag="PLY05_12395"
     CDS             complement(<1..1010)
                     /locus_tag="PLY05_12395"
                     /inference="COORDINATES: protein motif:HMM:NF027825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HNC04139.1"
                     /translation="MFDNLDLEKSKLIKNKSGNDFFADKPVSEINSQKSELDSEVTQE
                     LHARLMGFYRQELDRQAQNRYEMAIDEDYYDNIQWDDESAAILRQRGQAPLVYNVIST
                     SVRWVLGTERQAKSDYKILPRRKEESDAAEKKTSLMKYLSDVNRLPFARARAFKDAVI
                     TGLGWLEVGIQDETDNDEMIYSRYESWRNLLWDSAAIESDLSDARYMFRVKWVDMDAA
                     KAMFPNREAQLEAAAQESNRYASISSTDANGDIPMDYPEYSLEEQMTNRAISFNTRKR
                     VRLIECWYKMPSQEKRITKGVFKGEIYDPNEPRHQDYIEHLAERLTYVTHCCVMTVSD
                     ML"
     gene            complement(1010..>1432)
                     /locus_tag="PLY05_12400"
     CDS             complement(1010..>1432)
                     /locus_tag="PLY05_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04140.1"
                     /translation="TPVCAEQQPCIDLTHCHFRRAVGEFAVFGTWLKIGKDSEPCLVI
                     TPQRMFEGKPAVIPLSSAWKYSQMAFADPKVVANQVRQFAIGMNKDSLAQVIALTDLI
                     NDCLSDLISMPPDPRERLVVADAEIVIDGRKRTIEVTE"
BASE COUNT          372 a          349 c          270 g          441 t
ORIGIN      
        1 tacaacatat cgctgactgt catcacgcaa caatgcgtca catacgttaa acgctcggct
       61 aaatgctcaa tgtaatcttg atgccttggc tcgttggggt cgtaaatctc gcctttgaat
      121 acgcctttcg tgattctttt ctcttggctt ggcatcttgt accaacattc aatcagtcga
      181 acacgtttgc gcgtgttaaa actaatcgcc ctgttggtca tttgttcttc tagcgagtat
      241 tcgggatagt ccatcggaat atcgccattt gcgtctgtgc tgctgattga cgcataacga
      301 ttactttctt gtgctgcggc ttctagttgt gcttcacgat ttgggaacat agcctttgct
      361 gcatccatgt ccacccattt aacacgaaac atataacgcg catcacttaa atcgctttcg
      421 attgcagcac tatcccacaa cagattgcgc catgattcat aacgcgagta aatcatctca
      481 tcgttatctg tttcgtcctg aatacccacc tcaagccaac ctagtcctgt gatgactgca
      541 tccttaaatg ctcttgctcg tgcaaatggc aatctgttca catcacttaa atacttcatt
      601 aaagaagttt tcttttcagc cgcgtccgat tcttctttgc gtctcggcag aatcttgtaa
      661 tcagactttg cttgacgttc tgtacctaac acccaacgaa ctgacgttga gatgacgtta
      721 taaactaaag gtgcttgacc gcgttgacgc aaaatagcgg ctgattcatc gtcccactga
      781 atattgtcgt aataatcctc atcaatcgcc atttcatagc gattttgggc ttgtctatcc
      841 aactcttgac gataaaagcc catcaaacgt gcgtgtaatt cttgcgtgac ttcgctatct
      901 aattcgcttt tttgagaatt gatttctgat actggcttgt ctgcaaaaaa atcattgcct
      961 gatttattct taatcagctt acttttttca aggtctaaat tatcaaacat tactctgtta
     1021 cctcaattgt gcgttttctg ccatctatga caatctccgc atcagccacg actaggcgtt
     1081 ctcgtgggtc aggtggcata gagataaggt ctgataaaca atcattgatt aaatcagtca
     1141 aagcgataac ttgcgccaaa ctgtccttgt tcatgccaat agcaaactgt ctcacttgat
     1201 tagcgaccac tttagggtct gcaaaagcca tttgtgagta tttccaagct gaagacaaag
     1261 gaataacggc tggcttaccc tcaaacattc gctgtggtgt gataaccaag caaggctcac
     1321 tatctttacc aatctttagc catgtgccaa acacggcaaa ctcgccaaca gcacgcctaa
     1381 aatggcaatg cgttaaatca atgcagggtt gttgttcagc acaaacaggg gt
//
LOCUS       DAOQMB010000598         1430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74120_length_1430_cov_10.661818, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000598 DAOQMB010000000
VERSION     DAOQMB010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74120_length_1430_cov_10.661818"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /gene="lptD"
                     /locus_tag="PLY05_12405"
     CDS             <1..875
                     /gene="lptD"
                     /locus_tag="PLY05_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HNC04141.1"
                     /translation="NLESHVIFERENKHFLQTLEPHFLYSFSPYHNQTTLPNFDTATT
                     TFSYAQLFRSRRFSGGDRLEDANQLSVGISSRWSNTDTGIERFRASLGEVFYFRDRKV
                     QLNATDPTATASSSGLVGELAANFTDDWTSTADALWTADGDLSQFGLQLHFLPNNYTQ
                     LFNVGYSFRREVQALNQKELRQTNLSFVQPLNERWQLMGLWQYDLLNKETPEALIGAS
                     YEACCWQVSLYRRQFLADVDNNSSADRQRNAFFIELSLKGLAGLSSGARDLLKNRVFG
                     YSQLLEQQNPLKRN"
     gene            893..>1430
                     /locus_tag="PLY05_12410"
     CDS             893..>1430
                     /locus_tag="PLY05_12410"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNC04142.1"
                     /translation="MRHFLLLPLLLSPLFSYAASTPLDTIAAVVDEDVVLNSELEQRL
                     TDIRFQYRNRQQGLPPEDVLKKQVLEQLIIQRIQLSQAERAGVRIDDNSLNAYLNDVA
                     AQSGLSLADFRTKLESEKAGTYQEVREQIRQEMMISRLRNKRVGDRVRITEQDIDNFL
                     ASPQSAIALETEYRLAHLT"
BASE COUNT          423 a          278 c          280 g          449 t
ORIGIN      
        1 ttaatttaga aagtcatgtg atttttgagc gtgaaaataa gcactttttg caaacattag
       61 aaccgcactt tttgtatagc ttttcgcctt atcataatca aacgacatta ccgaattttg
      121 ataccgccac caccaccttt agttatgccc aactatttcg ttcacgacgc ttttcgggtg
      181 gtgatcgttt agaagatgct aatcaactgt ctgtcggtat tagctcccgt tggagcaaca
      241 ccgatacagg tattgagcga tttcgtgcct ctttaggtga agtattttac tttcgtgatc
      301 gcaaagtaca actcaatgcc acagatccaa cagcaacagc ttcttcatcg ggtttagtgg
      361 gtgagttagc cgcaaacttt acggatgatt ggacgagtac cgcagatgcc ttatggacag
      421 cggatggcga tctttcccaa tttggtttgc aattacattt tttaccgaat aactacacgc
      481 aattatttaa tgttggttat agttttcgcc gtgaagtcca agccttaaac caaaaagagc
      541 tgcgtcaaac taacttgtct tttgtccaac cgctcaatga acgttggcaa ttgatgggct
      601 tatggcaata tgacttatta aataaagaaa cacctgaagc cttaattggc gcaagttatg
      661 aagcgtgctg ttggcaagtt tctttatacc gtcgtcaatt tttagccgat gttgacaata
      721 atagctctgc tgatcgtcaa cgtaatgctt tttttattga actcagttta aaaggtttag
      781 caggtttatc tagcggtgcg cgcgatttat taaaaaaccg tgtatttggt tatagccaat
      841 tgttagagca acaaaaccca ctcaaaagaa attaacgaga cattgattat ttatgcgtca
      901 ctttttatta cttcctttat tattaagccc tttatttagt tatgctgctt ctactccgct
      961 cgataccatt gctgcggttg ttgatgaaga tgtggtttta aacagtgaac tcgagcaacg
     1021 tttaacagat attcgttttc aatatcgtaa tcgtcaacaa ggtttgccac ctgaagatgt
     1081 attaaaaaaa caagtacttg aacaattaat catacaaaga attcaattgt ctcaagctga
     1141 acgcgcaggg gtaagaattg atgacaacag tctaaacgct tacttaaatg atgttgctgc
     1201 tcaaagtggt ttgtctttag cagactttcg caccaaatta gaaagcgaaa aagctggaac
     1261 ttatcaagaa gtacgagaac aaattcgtca agaaatgatg attagtcgtt tgcgcaacaa
     1321 acgtgttggc gatagagtgc gtattactga acaagatatt gataactttt tagcctctcc
     1381 gcaaagtgcg attgctctcg aaaccgaata ccgtttggca catttaacca
//
LOCUS       DAOQMB010000599         1425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74619_length_1425_cov_10.020438, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000599 DAOQMB010000000
VERSION     DAOQMB010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1425
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74619_length_1425_cov_10.020438"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..899
                     /locus_tag="PLY05_12415"
     CDS             162..899
                     /locus_tag="PLY05_12415"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HNC04143.1"
                     /translation="MKPTYFISDLHLAADLPRVTQGFTQLLQQLHGKANALYVLGDLF
                     EVWVGDDNLSDYNQQIIRCFKQLTANGVALYFVHGNRDFMLGEAFATACGGQLLAERT
                     VINLGEQSILVEHGDALCTLDEKFMAFRAQSRHPLWQQGMLAKPLAERVQIAELWRLQ
                     SKMQNSNKPENIMDVTPQAVLDTLQQHQLTTLLHGHTHRPMIHQLTLNNGEAGQRIVL
                     GDWQEETGSAVIGIADEQGIRLEDYQF"
     gene            complement(1046..>1425)
                     /locus_tag="PLY05_12420"
     CDS             complement(1046..>1425)
                     /locus_tag="PLY05_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04144.1"
                     /translation="LASLTTAGAATFNAQHPNGVPTTACGEGAYTANGIKNYSWSGTS
                     SFTNVVDPLDYFFGLTGLAGTEPNDGLIGRCSSHFGKVIRDDYPWNHADAINHTFGVQ
                     GLFAPDPKDAYRQHANRLKNAGV"
BASE COUNT          460 a          288 c          271 g          406 t
ORIGIN      
        1 tactaacaca tataaaatct gttaaatctg aatcatacac ttgattcagc atcatagatt
       61 tcattcctca accgcacact acaaacatca ctaagcattt ataaattatc ttacaagaca
      121 tataatgaca acaaatagca actctcaata tataacacac catgaaaccc acttatttta
      181 tttctgactt acatttagct gccgatttgc cacgtgtcac tcaaggcttt acccagctat
      241 tacaacaatt acacggcaaa gcaaatgcgc tttatgtctt aggtgaccta tttgaagtat
      301 gggtgggtga cgataatctt agcgactata atcaacaaat tattcggtgt ttcaaacagt
      361 taacagcaaa tggtgtggct ttatattttg ttcatggcaa tcgggatttt atgctaggtg
      421 aagcatttgc gactgcgtgt ggaggccaat tattggctga acgtaccgtc attaacttag
      481 gtgaacaatc tattttagtt gaacatggtg atgctttatg caccttagac gaaaagttta
      541 tggcgtttag agcgcaaagt cgtcatccat tatggcaaca aggtatgtta gctaaaccat
      601 tagccgaacg tgtacaaatt gctgagttgt ggcgtttaca aagcaaaatg caaaacagta
      661 ataagcccga aaatattatg gatgtgacac cacaagcggt gttagatacc ttacaacaac
      721 atcaactgac caccctacta catggccata ctcaccgccc aatgattcat caattgactt
      781 taaataatgg cgaagcagga caacgtattg tattaggtga ttggcaagaa gaaactggca
      841 gtgcggttat tggtattgct gatgagcaag gtattagatt agaagattat cagttttagc
      901 ttcgactgca ctcagctaac acaactacct cagctatctt attaaaagcc tcggttatgg
      961 gctggctgag cgaaatctaa gccaaaaata caaacggcta cttaaagtag ccgttttgta
     1021 tttgccaaat agtcaatatt aagcgttaaa cacctgcatt ttttaggcgg ttagcatgtt
     1081 gacggtaggc atcttttgga tctggagcaa ataaaccctg tacaccaaag gtatggttaa
     1141 tcgcgtcagc atggttccaa ggataatcat cacgaatcac tttaccaaag tgactagaac
     1201 aacgaccaat taagccatca tttggctctg taccagctaa acctgttaaa ccaaaaaagt
     1261 aatctaatgg atcgactaca ttagtaaatg aagatgtgcc gctccatgaa tagtttttaa
     1321 taccattagc cgtataagct ccctcgccac aggcagttgt aggcacacca tttggatgtt
     1381 gagcattaaa cgttgcagca cctgctgttg ttaaagatgc taaag
//
LOCUS       DAOQMB010000600         1424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74723_length_1424_cov_12.971512, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000600 DAOQMB010000000
VERSION     DAOQMB010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74723_length_1424_cov_12.971512"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /gene="tilS"
                     /locus_tag="PLY05_12425"
     CDS             complement(<1..609)
                     /gene="tilS"
                     /locus_tag="PLY05_12425"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNC04145.1"
                     /translation="MLSTVTKFLTQHLNPNIQRLVIAYSGGVDSHVLLDMAVQVQQLH
                     QRPIKIIHVHHGLSQYANDWANHVEQVAQAYNVDYVIKRVSVNQQQASLEDAARQARY
                     QAINQELVIGDVLLLAHHQQDQAETFLLRLMRGSGAKGLAGMAVIASVPFNQLNIPAW
                     RPLLTVNKSQILDYAQQRQLNWIEDDSNTDERLNRNYLRHQVI"
     gene            complement(610..1035)
                     /locus_tag="PLY05_12430"
     CDS             complement(610..1035)
                     /locus_tag="PLY05_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005185353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC04146.1"
                     /translation="MVTAIKWLLDTNIISEAIKPIPDSNVINNLTLYSYQIAIAAPVW
                     HELNYGLLRLPDGKRKDMVKKYLENVVSTLTILPYDDVAAKCHAELRSIAQAKGQIIP
                     FVDGQIAAIAISHNLTLVTRNLQDYRMIEDLKISNWFST"
     gene            complement(1029..1301)
                     /locus_tag="PLY05_12435"
     CDS             complement(1029..1301)
                     /locus_tag="PLY05_12435"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNC04147.1"
                     /translation="MLALSLSQAKLHLSQAIAEAERQHQPVFISSHGKVKAVLLSIHD
                     YQQTQNQINFAERLQAWRDSYAQDMLIDDDIFTQTRSPEQGRDFSW"
BASE COUNT          420 a          283 c          264 g          457 t
ORIGIN      
        1 aatcacctga tgacgtaaat aattacggtt taaacgttca tcggtattgg agtcatcttc
       61 aatccagttt aattgacgtt gttgggcata atccaaaatt tggcttttat taaccgtcaa
      121 taaaggccgc catgcgggaa tattgagttg attaaatggc acagaagcta tcacagccat
      181 tccagccaaa cctttagcac ctgagccacg cattagacgc aataaaaatg tttcggcttg
      241 atcttgttga tgatgagcta ataacaacac atcccctatc actaactctt ggttaatggc
      301 ttgataacgt gcttggcgag cggcatcttc tagactggct tgttgttggt taacgctgac
      361 ccgtttaatg acatagtcca cgttataagc ttgagcaact tgctcaacgt gattcgccca
      421 atcgttagca tattggctta aaccatgatg cacatgaata attttaattg gacgttggtg
      481 aagttgttgt acttgcacag ccatatctag taaaacgtga gagtcaacgc caccactata
      541 agcgatgact aaacgttgga tatttgggtt taggtgttgg gtgagaaatt ttgtgaccgt
      601 agatagcatt taggtactaa accagttgct aatttttaaa tcttcaatca ttcgataatc
      661 ttgcagatta cgcgtaacca atgttaagtt gtggcttatg gcaattgctg ctatttgtcc
      721 atcaacaaaa ggtataattt ggcctttagc ctgagctata ctgcgtaact cggcgtgaca
      781 ttttgccgca acatcatcat aaggcaaaat agttaaggta ctcaccacat tttctaaata
      841 ttttttaacc atatctttgc gctttccatc aggcaaacgc aacaatccat aattaagttc
      901 atgccatact ggtgcagcta tcgctatttg ataactatac aatgttaaat tatttatcac
      961 gttgctatca ggaattggtt taatagcctc agaaataata ttggtatcca ataaccattt
     1021 tatcgctgtc accatgaaaa gtctcgacct tgctcaggac tacgagtttg ggtaaatata
     1081 tcgtcatcta ttagcatatc ttgagcataa ctatcacgcc atgcctgcaa gcgttcagca
     1141 aaatttattt gattttgtgt ctgttgataa tcatgtattg acaataacac ggctttaact
     1201 ttgccatgac tagaaataaa tacaggctga tgctgacgtt ctgcttcagc aatcgcttgc
     1261 gacaaatgta gtttggcttg agataatgat aaagctaaca tttacgttct cctctacaaa
     1321 attccataac tcataatacg ttctaaacga cgctcacata aggttttttc atccatactg
     1381 cgtaaacgtt taagttgttt tactaaggtt ttacgcaagg tatc
//
LOCUS       DAOQMB010000601         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74806_length_1423_cov_17.944444, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000601 DAOQMB010000000
VERSION     DAOQMB010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74806_length_1423_cov_17.944444"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            20..1291
                     /gene="tolB"
                     /locus_tag="PLY05_12440"
     CDS             20..1291
                     /gene="tolB"
                     /locus_tag="PLY05_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011182570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HNC04148.1"
                     /translation="MRSLRLFFFALIGITASHSYGEITFDIIKPSANTTPIAIVPFAN
                     DTNSETLNTIISQDLVRSGLFKPADKFPEKPSNSADINYGVWQAVNLDYVVVGTVTPQ
                     VNNQLTIQYELLSSNQQQRLLGESMTIPPARWRDAAHFISDRIFKQITGFDGSFSTRV
                     AYVLQYKQAGQTRYRLDIADADGRRPTTVLDSEEPILSPSWSPDGQKMAYVSFESGKP
                     AIYSQNLITQERQVISRLKGLNGAPAWSPDGSKLALTLSQDGNPEIYVFEIASKKLTR
                     LTNHYAIDTEARWYPDGRGLIFTSDRGGSPQIYRMDLASQEVKRLTFEGSFNGRGAIS
                     PDGKYLAMVHRRAGQSFQIAIQDLQTGGLTRLTETPLDEAPSFSPNSQMIVYATRQGN
                     KGVLGIMSLDGRFKMQLPASEGQVREPAWSP"
BASE COUNT          415 a          304 c          290 g          414 t
ORIGIN      
        1 tttagcagca aggactaaca tgcgctctct tcgtttgttc ttttttgcct taattggcat
       61 aaccgcctca cacagctatg gcgaaataac gtttgatatt attaaaccga gtgcaaatac
      121 cacgccgatt gccattgtgc cttttgccaa cgacaccaac agcgaaacct taaataccat
      181 tattagtcag gatttagtac gtagtggttt atttaaacct gctgataaat ttcctgaaaa
      241 accaagcaat agtgccgaca tcaattatgg tgtgtggcaa gcagttaatt tagattatgt
      301 ggtggttggc acagtcaccc cccaagtcaa taatcaatta actattcaat atgaattatt
      361 aagcagcaat caacaacaac ggctgttagg cgaaagcatg actattccgc ctgcacgttg
      421 gcgagatgct gcacacttta ttagcgaccg tatttttaaa caaattactg gatttgatgg
      481 ttcttttagc actcgtgttg cctatgtttt gcagtataaa caagctggcc aaacccgtta
      541 tcgactcgat attgctgatg cggatggtcg tcgtcccacc acggttttag atagcgaaga
      601 accgattttg tcaccaagtt ggtcgcctga tggccaaaaa atggcctatg tttcctttga
      661 aagtggcaaa cccgctattt atagccaaaa tttaattacc caagaacgtc aagttattag
      721 tcgtttaaaa ggcttaaatg gcgcacctgc ttggtcgcct gatggctcaa aacttgctct
      781 aactttatct caagatggga atcccgaaat ttatgtattc gagattgcta gcaaaaaact
      841 aacaagactt accaatcatt acgcaattga taccgaagca cgttggtatc ctgatggccg
      901 tgggttaatc tttacctcag accgaggtgg cagcccacaa atttatcgta tggacttagc
      961 tagccaagaa gtgaagcgtt taacctttga aggctctttt aatggtcgtg gtgccattag
     1021 tcctgatggt aaatatttag cgatggtaca tcgcagagca ggccaaagtt ttcaaattgc
     1081 tattcaagat ttgcaaacag gtggattaac acgactcaca gagacgccat tagatgaagc
     1141 accaagcttc tccccaaata gtcaaatgat tgtttatgcc acacgacaag gcaacaaagg
     1201 ggtgttaggc attatgtctt tagatggtcg ttttaaaatg caacttcctg cttccgaagg
     1261 acaagtacgt gagcctgcat ggtcgcctta attcttttac attttacgct ctaattcatt
     1321 gggagaaatg acacatgtta aacagtaaac ttagtttagt cttagccatc agtttagcga
     1381 ttggcgtgac tggctgtagc accctaaaaa agtctactac cac
//
LOCUS       DAOQMB010000602         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74818_length_1423_cov_5.530702, whole genome shotgun sequence.
ACCESSION   DAOQMB010000602 DAOQMB010000000
VERSION     DAOQMB010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74818_length_1423_cov_5.530702"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLY05_12445"
     CDS             complement(<1..280)
                     /locus_tag="PLY05_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="cytochrome c-type biogenesis protein; required for
                     the transfer of heme to apocytochrome c; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HNC04149.1"
                     /translation="MIAELGHFALILGLVMALLQSTLPLYGVYAANVNLQRSARSAAL
                     LQAFCLSLSFLGLTIAFLQNDFTLDYVSRQSNTLLPYYYKISAVWGGHE"
     gene            602..994
                     /locus_tag="PLY05_12450"
     CDS             602..994
                     /locus_tag="PLY05_12450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016652180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNC04150.1"
                     /translation="MFISSFSPRFYETDAFGHVNNTVVTGWFETAREPIFRIFTPEMD
                     IKALSMILARIEVDYVAQIYYGQDVLVHTGIEKIGNSSFVVWHEAYQNEQLVARGKAV
                     QVHFDFASQKSLALPDNLRQQLAAHLRV"
     gene            complement(1062..>1423)
                     /locus_tag="PLY05_12455"
     CDS             complement(1062..>1423)
                     /locus_tag="PLY05_12455"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC04151.1"
                     /translation="QPNKKLADGRDMPAVQGLLATANNHLRAGKLEEAASSLERAQRL
                     APQSATVYQRLAEVRLQQGRTLEAEQLARKGLSYAQGNAQQAALWYLVAEAAERQGKQ
                     DAAIQAKNKAIQLDILH"
BASE COUNT          411 a          297 c          267 g          448 t
ORIGIN      
        1 cttcatgtcc gccccatacc gcagaaattt tataataata aggaagtaag gtattggact
       61 gccgagacac atagtcgagg gtgaaatcat tttgcaaaaa agcaatcgtt aatcctaaaa
      121 aagataaact caaacaaaac gcttgtaaca acgctgccga acgtgcgcta cgttgtaaat
      181 taacattggc cgcataaacg ccatacaaag gtaatgtcga ttgtaacaac gccattacta
      241 gtcccaaaat cagggcaaaa tgacctaact cagcaatcat ctaaggctcc cataggcaga
      301 gggaatttaa tcagcgcgca taatacgcac taaacgtaga ttgtctaggc tattatcgtt
      361 attatttttc tttaaatcgt catacagcaa taacacaact tacttttagg atttaaacgc
      421 acaggatttt ttgacatttg aattgttatt gtccgttaat actattttca tttgcgattg
      481 gctcgactag gctgaagcac gactttaaca ctattttagg atttacaccg ttatcaatta
      541 tgtagcgaac aaaagttaat ttcggtgaaa tgagtaagtc ctttattaat gagccatctt
      601 tatgtttatt tcttcttttt caccacgctt ttacgaaaca gacgcttttg gccatgtgaa
      661 caatactgta gtcacaggtt ggtttgaaac agcacgtgaa ccgatttttc ggattttcac
      721 ccccgaaatg gatatcaaag ccttgagtat gattttagcg cgtattgaag tagattatgt
      781 ggcacaaatt tattacggcc aagacgtatt agttcatact ggcattgaaa aaataggtaa
      841 ttcatcattt gtggtttggc atgaggctta tcaaaatgag cagttagttg ctcgtggcaa
      901 agcagtacaa gtacattttg attttgccag tcaaaaatca ctggccttac ccgacaactt
      961 acgtcaacaa ttagccgcac atttacgcgt ttaacctcga tcacctgcca tctctaactg
     1021 catcgcttta tttttggctt gaatatctaa tttctattta tttaatgtag tatgtctaac
     1081 tgtatcgctt tatttttggc ttgaatggcc gcgtcttgtt taccttgacg ttcggccgcc
     1141 tctgccacca aataccataa agcagcttgt tgggcattac cttgtgcata cgacaaacct
     1201 ttacgcgcta attgttcggc ctcaagggtt cgaccttgtt gtaaacgcac ttcggcaagg
     1261 cgttgataaa ctgttgctga ttgtggtgct aaacgttgtg cgcgttctaa actgctcgct
     1321 gcttcctcta atttacccgc acgtaaatga ttgttagcag tagccaataa accttgtacc
     1381 gcaggcatat ctcgaccatc tgccaatttt ttattaggtt gaa
//
LOCUS       DAOQMB010000603         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74827_length_1423_cov_4.347953, whole genome shotgun sequence.
ACCESSION   DAOQMB010000603 DAOQMB010000000
VERSION     DAOQMB010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74827_length_1423_cov_4.347953"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..482
                     /locus_tag="PLY05_12460"
     CDS             180..482
                     /locus_tag="PLY05_12460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HNC04152.1"
                     /translation="MNMHNPAHAGQLVQEWLDGLKEEGTPITLTQLAKSISVTRTALS
                     RMIHGHTTLTADMALRLQAALGMNADLLMRVQASYEIWQAAQQTRPHIELLVAHHH"
     gene            complement(565..822)
                     /locus_tag="PLY05_12465"
     CDS             complement(565..822)
                     /locus_tag="PLY05_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04153.1"
                     /translation="MLLITPKPLFSATGVCGVVLFVLVLLFVELLTAGSLLGGVLDVL
                     SPPPPPPQAAIANDNNVSINGLVWFGLVWFGLVWFAAYNYS"
     gene            836..>1423
                     /locus_tag="PLY05_12470"
     CDS             836..>1423
                     /locus_tag="PLY05_12470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04154.1"
                     /translation="MVNASVAKAGRFLFKWDFVPNAGYYRLMKSVDGKQPYQQVGEKL
                     LTNSTTEDISTFEQDWANANYIVQACDAEGCSLSNKIYSGNVMLDMIAYVKDKAFSQK
                     YDELVKSSMNSNISSLMTIHETIPSSFNIYQKINFTQQCSVSIPRGDGTYMAGVGSCP
                     SAYQKNFLFSNTVSGDDWRFVESNDSKPKSLVTYQD"
BASE COUNT          453 a          234 c          309 g          427 t
ORIGIN      
        1 agggttggtg gataagttgg atagatagta gcgtgggttg aggtacgaaa cccacgataa
       61 cgaaatgtca attgtgggtt tcctgcgtca acccacacta cttatactta tactttttgt
      121 tttgaagatg gcaatgcgta tattgttgat tatcaagatt atcattgagg tttaacacca
      181 tgaatatgca caatcctgcc catgcagggc aattggtaca agagtggtta gacggtttaa
      241 aggaagaggg aacaccaatt acattaacgc aacttgctaa gagtatatcg gtgacacgta
      301 cagcattgtc acgtatgatt catggtcata cgacattaac cgcagacatg gctttacgct
      361 tacaggcagc attaggaatg aatgctgatt tattaatgcg tgtacaagca agttatgaaa
      421 tttggcaagc cgctcagcaa acaagaccac atattgaatt attagttgcg catcatcatt
      481 gaggtttaga atattaataa attgtaaaaa acgtctcgta aataattaaa tttggtttat
      541 aatttgtacg attgttgttg ttttttagga atagttatat gctgcaaacc aaaccaaacc
      601 aaaccaaacc aaaccaaacc aaaccaaacc attaatactg acattattgt cattagcgat
      661 tgctgcttgt ggcggtggtg gtggcggtga gagtacgtcc aatacgccac ctaacaatga
      721 tccagcagtt aataactcaa caaataacaa cactaataca aataatacaa cgccacaaac
      781 acctgttgct gaaaacaacg gtttgggggt aattaataac aaattggctg cgcctgtggt
      841 aaatgcttct gtggccaaag cagggcgttt tttgtttaag tgggattttg tgccaaatgc
      901 gggctattac cgtttaatga agtcagttga tggcaagcaa ccttatcagc aagttggcga
      961 aaagctgtta actaattcga ctaccgaaga tatttcgaca tttgagcaag attgggcaaa
     1021 tgcaaattat attgtgcaag cgtgtgatgc cgaaggctgt tcgttgtcaa ataaaattta
     1081 ttcgggcaat gtgatgttgg atatgattgc ttatgttaag gataaagctt tttcgcagaa
     1141 gtatgatgag cttgttaaaa gctctatgaa cagcaatatc agttcattga tgactataca
     1201 tgagacaata ccttctagtt ttaatatata tcagaagatt aattttactc agcagtgtag
     1261 tgttagtatt ccgcgtggag atggaactta tatggcaggt gttggttcat gtccatcagc
     1321 atatcaaaaa aacttcttat tttctaatac ggtgagtggt gatgattggc ggtttgtgga
     1381 gtcgaatgat agtaaaccta agagtttagt tacttatcaa gat
//
LOCUS       DAOQMB010000604         1423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_74894_length_1423_cov_2.059211, whole genome shotgun sequence.
ACCESSION   DAOQMB010000604 DAOQMB010000000
VERSION     DAOQMB010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74894_length_1423_cov_2.059211"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PLY05_12475"
     CDS             <1..506
                     /locus_tag="PLY05_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNC04155.1"
                     /translation="GSGSLLLKFAKVLGKENAKLLYGQEEAILAYQAAVDDFLKTTAR
                     SLSLTVRQQQLQQFKQQYLAEYLPVLNSYQEKGFNRYLEDLALAELDSQNNEQLAAIR
                     LKTRQYYFGEQKAQQMAQQDQTQAEQQQRMAQYQHAKQQLLTKYGNNSNDSQYLAQLQ
                     QLRQQLF"
     gene            540..>1423
                     /locus_tag="PLY05_12480"
     CDS             540..>1423
                     /locus_tag="PLY05_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HNC04156.1"
                     /translation="MALTRLISLLMLLLTSTLSWATSYIYVTNTTPEVVSVSSVQYGD
                     KQLTKGTQWGTYDTVIPAYATRKILWMNRNTGITSGKYFYFDTTITANGSQVVLQQKL
                     KGNLIGSSIWHSAKASSFSNPWFDDRNIHNNNFSYNNRNSVMSFKAEATSGYDDFYYV
                     THNNNHVEPVSSNANELKVLTYNLWTLIGAELKCERLKDVASAVKAYDVIVFEEAFEN
                     SCREQLMNDIRAEYHYQTRVVDNTSNILEDGGVIAVSRHPIAVEDQRVFPNCNGYICL
                     SNRGFVYLEVIKNGVPYHV"
BASE COUNT          449 a          292 c          274 g          408 t
ORIGIN      
        1 gtggctctgg ttcgttgttg ttgaagtttg ctaaggtatt gggtaaagaa aatgccaaat
       61 tactttatgg ccaagaggaa gcgattttag cctatcaagc agcggttgat gactttctca
      121 aaaccacagc tcgctcttta tcgctcacgg ttcgccaaca gcaattgcaa caatttaaac
      181 aacaatattt agccgagtat ctgcccgtat taaacagtta ccaagaaaaa ggctttaatc
      241 gttatttaga agatttagcc cttgccgaat tagatagcca aaacaacgaa caattagccg
      301 ctattcgctt aaaaacgcgc caatactatt ttggtgaaca aaaagcccaa cagatggcac
      361 aacaagatca aacacaagcc gaacaacaac aacgtatggc gcaatatcaa cacgccaaac
      421 aacaactgct cactaaatat ggcaacaact ctaatgacag ccaatattta gcccagcttc
      481 aacaactacg ccaacaatta ttttaatcag acaacattct gttttttcag gagtatttta
      541 tggccttgac ccgtctaatt agcctactga tgttactgtt gaccagtact ttatcatggg
      601 cgaccagtta tatttatgtg accaacacca cccccgaagt ggttagcgtt tcaagtgtgc
      661 aatatggtga taaacaactc accaaaggca cacagtgggg aacttatgac acggtcattc
      721 cagcctatgc aacccgcaaa attttatgga tgaaccgtaa tacgggtatt actagtggta
      781 aatattttta ttttgacacc acgattacgg ccaatggctc acaagtggta ttgcaacaaa
      841 aactgaaagg taatttgatt ggctcgagca tttggcactc tgcaaaagca tcaagcttta
      901 gcaatccttg gtttgatgat cgcaacattc ataacaataa ctttagttat aacaaccgca
      961 atagtgtgat gtcttttaag gccgaagcca cctcgggtta tgacgatttt tattatgtga
     1021 ctcacaacaa caatcatgtt gagcctgtct ctagcaatgc caatgaatta aaagtattaa
     1081 cgtataattt gtggacgcta attggtgcag aattaaagtg cgaacgctta aaagatgttg
     1141 cctcagcagt caaagcttat gatgtcattg tctttgaaga agcttttgaa aattcttgcc
     1201 gtgaacaact catgaatgat attcgtgctg aataccacta ccaaactcgt gtagttgata
     1261 acaccagcaa tattttagaa gatggtgggg tgattgcggt tagtcgtcat ccgattgccg
     1321 ttgaagatca acgcgtattc cctaactgta atggctatat ttgtttgtct aatcgtggtt
     1381 ttgtctattt agaagtgatc aaaaatggtg tgccttatca tgt
//
LOCUS       DAOQMB010000605         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75236_length_1419_cov_10.583578, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000605 DAOQMB010000000
VERSION     DAOQMB010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75236_length_1419_cov_10.583578"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1304)
                     /locus_tag="PLY05_12485"
     CDS             complement(36..1304)
                     /locus_tag="PLY05_12485"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNC04157.1"
                     /translation="MLVMLLIMMALLGAPLFVVLAGAAAIGFINQDTSLTVLGVELYR
                     LVDTPILLALPLFTYAGYLLGEGNTSQRLVRLIRALLGWMPGGLAIVAFCTCAFFTAF
                     TGASGATIVALGALLYPALKQAGYTEKFSLGLVTTSGSLGLLLPPSLPLILYAIIAQQ
                     LPQGSGVSIEAMFKAGLLPALVMIVLLSIYGYWITRKNPIPLQQFSWTELKQATAEAK
                     WELPMPIFVLGGIYGGFFAVSEAAAVTALYVTVMTVLIYREISLTQLVRIMREAMVMV
                     GAILLILGVAMAFTNWLIEAEVPTKLFELVQQYIHSKWTFLLLLNIFLLILGCFLDIF
                     SALVIMVPLLLPMAIGYGVNPVHLGIIFLANLELGYCTPPVGMNLMIASYRFKRSMIE
                     LYQAAIPFLLVLIVAVLIITYVPWLSIGWF"
BASE COUNT          533 a          324 c          226 g          336 t
ORIGIN      
        1 aggagaactc aataaagttc tcccaatccc ttgccttaaa accaaccaat actcaaccac
       61 ggcacataag taataatcaa cactgcaacg attaacacca acaaaaaggg tattgccgct
      121 tgatacaact caatcatgga acgtttaaaa cgatagctgg caatcattaa attcataccc
      181 acaggtggcg tacaataacc taattctaga ttagccaaaa agataatacc caaatgaaca
      241 ggatttaccc cgtaaccaat cgccattggt aataataatg gcaccataat gactaatgcc
      301 gagaaaatat ctaaaaaaca gcctaaaatc agcaaaaaga tatttaacaa cagcaaaaac
      361 gtccatttac tatgaatata ttgttgaact aattcaaata gcttagtagg gacttcagcc
      421 tcaattagcc aattggtaaa ggccatcgca acgcctaaaa tcaatagaat tgctccaacc
      481 atgaccatcg cttcacgcat aatacgcaca agctgagtta aagaaatttc ccgataaatt
      541 aatacagtca tcaccgtaac atacaaagct gtcactgctg ctgcttctga cactgcaaaa
      601 aaaccaccat aaataccacc taagacaaaa ataggcatgg gtaattccca tttggcttcg
      661 gctgttgctt gttttaactc agtccaagaa aactgttgta atggaatagg atttttgcgt
      721 gttatccaat aaccatatat agataacaat acaatcatca ctaaggcagg tagcaaaccc
      781 gctttaaaca tcgcctctat gctaacacca gagccttgtg gtaattgttg ggcaataatt
      841 gcatatagaa ttaatggtaa tgatggtggc aataacaagc ctaaagaacc tgatgttgtt
      901 accaatccca aagaaaattt ttcggtgtat cctgcttgtt ttaaggctgg gtataataat
      961 gcccctaaag cgacaatagt cgcaccactt gcgccggtaa acgcggtaaa aaaagcacac
     1021 gtacaaaaag caactatcgc caaaccaccc ggcatccatc ctaataaagc acgaattaga
     1081 cgtaccaaac gttgagaagt attaccctca cccaataaat aaccagcata agtaaataaa
     1141 ggcaaggcta ataaaatagg tgtatcgact aaacgatata actcaacacc taataccgtt
     1201 aatgacgtat cttgattaat aaaaccaata gccgccgcgc ctgcaagcac cacaaataag
     1261 ggcgcaccta acaacgccat catgattaat aacataacta gcatgaaggt cacgttgtag
     1321 attccttgag ttctgcatta attaacaata ataaagactg acaggtaaac cgaattgcca
     1381 ttagtgcaaa accaatcggc ataatgcttt cacaccacc
//
LOCUS       DAOQMB010000606         1419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75237_length_1419_cov_9.652493, whole genome shotgun sequence.
ACCESSION   DAOQMB010000606 DAOQMB010000000
VERSION     DAOQMB010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75237_length_1419_cov_9.652493"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1398
                     /locus_tag="PLY05_12490"
     CDS             91..1398
                     /locus_tag="PLY05_12490"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNC04158.1"
                     /translation="MLHKIAKNFSLINEQDSPQTIHDNILSGIVFKGTNLWILMVAIL
                     MASVGLNVNSTAVIIGAMLISPLMGPIIGMGYGLGTYDFDLVRQSIKNYLFAVVIGLI
                     VSTLYFSISPINEAHSELLARTTPTIYDVLIAFFGGLAGFIAIVSRFKGNVITGVAIA
                     TALMPPLCTAGYGLASQQFNFVSGALYLLAINTVFIALAALITTRLFGQPLIQQANDE
                     QKKQANRYVTFIVVLTLIPSIYLGVSFIHQDQFQRQAKNFVNTIKLDNNYLLKYEVLP
                     TRKVINLTFGGMGLSVEQVQMLQQQAIEQGLDAKINTQQGFSFSQLGDINNQTELLRK
                     EVNHLREDLTKLAQAERQKNTLEVNLANTLLTELQVFYPDIVSVELGNISESTINQGA
                     TQFVIIRTQSPILKQNLPQKTIQSWLKQRLQQQNMIVVINPSL"
BASE COUNT          430 a          279 c          246 g          464 t
ORIGIN      
        1 aaattcaatg attacttaca attttaaaat tgaccagatt cctcactatc cattacttaa
       61 gtttacttat tttttgctga gtaagcacaa atgttgcata aaatcgctaa aaactttagc
      121 ttaatcaacg agcaagactc gccacaaact attcatgaca atattttgag tggcatcgtt
      181 tttaaaggca caaacctatg gattttgatg gttgccattt taatggctag cgttggctta
      241 aatgttaact ccacagcagt gattattggt gccatgttaa tctctccctt aatggggcct
      301 attattggca tgggttacgg tttaggcacc tatgattttg atttagtccg ccaatccatt
      361 aaaaattatt tatttgctgt tgtcattggg cttattgttt caaccttgta ttttagcatt
      421 tcccctataa acgaggctca ctccgaactt ttagcccgta ctacgccaac gatttatgat
      481 gtattaattg ctttttttgg cggtttagcc ggttttattg ccattgttag tcgttttaaa
      541 ggcaatgtca ttactggtgt cgccattgcc accgcattaa tgccgcccct atgcaccgca
      601 ggctatggcc tagcatcaca acaatttaat tttgtttcgg gggctttata tttattagcc
      661 attaatactg tttttattgc tctagcggct ctcattacca cacgtttatt cggtcaaccg
      721 ctgattcagc aagccaatga tgaacaaaaa aagcaagcca atcgttatgt gacatttatc
      781 gtcgtcttaa ccttgattcc aagtatttat ttaggggtca gttttattca tcaagatcaa
      841 tttcagcgac aagccaaaaa ttttgttaat accattaaat tagacaacaa ttacctgctt
      901 aaatatgaag tattaccaac acgcaaagta attaacctta cctttggtgg aatggggtta
      961 agcgttgagc aagtacaaat gctccagcaa caagccattg agcaaggttt agatgctaaa
     1021 attaacaccc aacaagggtt ttctttttcc caattaggtg acataaacaa tcaaaccgaa
     1081 ttacttcgca aagaggtcaa tcatttacgt gaagatttga ctaagcttgc tcaagcagaa
     1141 cgacaaaaaa acacgcttga ggtgaattta gcgaatacct tgttaactga attacaagta
     1201 ttttatccag atattgttag cgtggaattg ggcaatatta gtgaatctac tatcaatcaa
     1261 ggagctacgc aatttgtgat tattcggact caaagcccta tactcaagca aaatttgcca
     1321 caaaaaacca ttcagtcttg gctcaaacaa cgactccaac agcagaatat gattgttgtt
     1381 attaatccat cgttatagga tatttaagcc gtgtatatg
//
LOCUS       DAOQMB010000607         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75773_length_1414_cov_23.673289, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000607 DAOQMB010000000
VERSION     DAOQMB010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75773_length_1414_cov_23.673289"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..667
                     /locus_tag="PLY05_12495"
     CDS             128..667
                     /locus_tag="PLY05_12495"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HNC04159.1"
                     /translation="MNKSESIAAISQAIAKAQHDVENASKNAKNPHFKSKYADLAELL
                     NTVRPVFSGHGLAIVQMPSFVDGVASVETLISHSSGEWISSICSAPVSKQDAQGVGSA
                     ITYLRRYSLAAMCGIAQEDDDANSAVGHAPKKKELPTLDDERFNHAVDAVESGKFPKM
                     RLLTEYALTDEQKRIAGAL"
     gene            667..1278
                     /locus_tag="PLY05_12500"
     CDS             667..1278
                     /locus_tag="PLY05_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04160.1"
                     /translation="MKIRCSQLKLLMTAPRLKSEVLSETAKTLIKDLVKESVFGVRKM
                     ITSKAIEKGIRCEDASIDLLNSVLFESYVKHVGRVSNEYITGECDILTKTSVRDIKTS
                     WSIDTFPMFKDDMGNDYEWQMRGYMWLYDRDCAIVDYCLVDTPEDLIGYEQRDLHIVS
                     HINPIHRVRSIAYNRDGQKEQLIIDKVTAAREYYNELLEQLAR"
BASE COUNT          457 a          265 c          318 g          374 t
ORIGIN      
        1 ttgttttgtg tcagtgcaat gtaatataaa cattttataa aatcaatata aaatacataa
       61 aaaataacta gacaaaaaaa ggcttaattg ctacatttgc acgacgtaaa caaaaaaaga
      121 ggttattatg aataagtcag aatctattgc agcaattagc caagcaatcg ccaaagctca
      181 acatgatgtt gagaacgcaa gcaaaaacgc aaaaaatcct cactttaagt ccaagtacgc
      241 ggacttagct gagttattaa acacggttcg tcctgtgttc agtggacatg gtttggccat
      301 tgtacaaatg ccatcgtttg ttgatggtgt cgcaagtgtt gagacgttaa tcagtcactc
      361 atcaggtgaa tggatcagtt caatttgcag cgctccagtg agtaaacaag acgctcaggg
      421 tgtaggcagt gcaatcactt atttgcgtcg ttattcatta gctgcaatgt gtggcattgc
      481 tcaagaagac gatgatgcca atagtgctgt tggtcatgcg ccaaagaaaa aagaattacc
      541 cactttagac gatgagcgtt ttaatcatgc cgttgacgcc gttgaaagtg gaaaattccc
      601 aaaaatgcgt ttactcactg agtacgcatt gacagacgaa caaaaacgca ttgcaggggc
      661 tttataatga aaattagatg cagtcagcta aagctgttaa tgactgcacc tcgtttaaaa
      721 agcgaggtgc tttccgagac ggccaagact ttgattaaag acttggtaaa agagtctgtt
      781 tttggtgtgc gtaaaatgat tacatcaaaa gctatagaga agggcattag atgcgaagat
      841 gcaagtattg acctattaaa tagcgttttg tttgaatctt acgtcaaaca cgttggccga
      901 gttagcaatg agtacatcac tggcgaatgt gacattttaa ccaaaactag cgtgcgtgac
      961 atcaaaacaa gctggagcat tgacacattc ccgatgttta aagatgacat gggcaatgat
     1021 tatgagtggc aaatgcgagg ctatatgtgg ctatatgacc gtgattgtgc gattgttgat
     1081 tattgcttag ttgacacgcc tgaagattta atcggctatg agcagcgtga tttgcatata
     1141 gtcagtcaca ttaacccgat tcaccgtgta cgctcgattg cttacaatcg ggacgggcaa
     1201 aaagagcagc taatcattga taaagtgacg gcagcgcgtg agtattacaa tgagctactt
     1261 gagcagttag caagataact acaccgctaa ggggtaacgc cccacagcaa actaaaacct
     1321 gagacgtacc tcagaatcat caaaaaatgc cccataagtg gctggggcgt tatcccactt
     1381 gagcgtaaag ttaggttgcc caccctaatt ttga
//
LOCUS       DAOQMB010000608         1414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75788_length_1414_cov_4.553348, whole genome shotgun sequence.
ACCESSION   DAOQMB010000608 DAOQMB010000000
VERSION     DAOQMB010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75788_length_1414_cov_4.553348"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLY05_12505"
     CDS             complement(<1..276)
                     /locus_tag="PLY05_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04161.1"
                     /translation="MNPHASSPTSLLALIRSFIKHRQLIFQMIQREVVGRYKGSVVGL
                     GWSFLNPVLMLTVYTFVFSVVFKARWGVDASESKTQFAVVLFVGLIVH"
     gene            complement(273..824)
                     /gene="rfbC"
                     /locus_tag="PLY05_12510"
     CDS             complement(273..824)
                     /gene="rfbC"
                     /locus_tag="PLY05_12510"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HNC04162.1"
                     /translation="MKIIDTAISEVKIIEPTVFGDERGFFYESFNQTKFENAIGYAVN
                     FVQDNHSRSVKGVLRGLHYQLPPHAQAKLVRCVVGEVFDVAVDIRRSSPTYGQWVGVH
                     LSAENKRQLWIPEGFAHGFLTLSDSAEFLYKTTNYYAVQSECLIRWNDTDLAINWPVI
                     DIDFQLSDKDKNGKSFSVAEVFE"
     gene            complement(828..>1414)
                     /locus_tag="PLY05_12515"
     CDS             complement(828..>1414)
                     /locus_tag="PLY05_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC04163.1"
                     /translation="NGAPSALVLGDNIFYGHEFSKLLQDANAKTTGASVFAYHVQDPE
                     RYGVVEFDANGRAMSIEEKPKQPKSNYAVTGLYFYDEKVVEIAKSIKPSARGELEITD
                     VNSAYLQAGALDVQTMGRGYAWLDTGTHESLLEASQFIATIEARQGLKVACPEEIAFR
                     SGWIDAEQLSRLAEPLKKNAYGQYLLSVLKERVF"
BASE COUNT          467 a          326 c          221 g          400 t
ORIGIN      
        1 atgcactatc aagcccacaa ataacacaac ggcaaattgt gttttgcttt cgctcgcatc
       61 aaccccccag cgcgccttaa acacaacact aaacacaaat gtatagacgg ttagcattaa
      121 aacaggattt aaaaacgacc atcccaatcc aacaactgag cccttatagc gcccaacaac
      181 ctcacgttga atcatttgaa aaataagttg tcggtgttta ataaaactgc gaatcaatgc
      241 aagtaacgat gtaggtgatg acgcatgggg attcattcaa aaacctcagc tacactaaat
      301 gacttaccat ttttatcttt atctgataat tgaaaatcaa tgtctattac aggccaatta
      361 atggctaagt ctgtatcatt ccaacgaatc aaacactctg attgaaccgc ataataatta
      421 gtcgttttat acaaaaactc tgctgaatct gacaaagtca aaaatccatg tgcaaagcct
      481 tctggaatcc ataattggcg tttattttct gcagataaat gtacgccaac ccattggcca
      541 tatgtaggag agctgcgacg aatatcaaca gccacgtcaa acacctcacc tacaacgcaa
      601 cgcaccaatt ttgcttgtgc gtgtggtggc aattgataat gcaagcctcg caagacacct
      661 tttactgaac gagaatgatt atcttgaaca aaattgacag cataaccaat ggcattttca
      721 aatttagttt gattaaaact ttcgtaaaaa aacccacgtt catcaccaaa cacagtgggc
      781 tcgatgattt ttacttcact aattgcggta tcgataattt tcacagttta aaacactctt
      841 tcttttaaca ccgacaataa atactggcca tacgcattct ttttgagtgg ctctgctaaa
      901 cgacttaatt gttcagcatc aatccatcca gagcgaaaag caatttcttc aggacaagcc
      961 acttttaaac cttgtctagc ttcaattgta gctataaatt gactagcttc taacaaagac
     1021 tcatgtgtgc ctgtgtccaa ccatgcataa cctctcccca tagtttgcac atctaaagca
     1081 ccggcctgca aataagcaga gttgacatcg gtaatttcta actcaccacg agcagatggc
     1141 ttaatagact tggcgatttc gactactttt tcatcataaa aatataaacc agtaacagca
     1201 taatttgatt ttggttgttt aggcttttct tcaatactca ttgcacgccc attagcatca
     1261 aactctacaa caccataacg ctctggatct tgaacatgat aagcaaaaac acttgcgcct
     1321 gttgtctttg cattggcatc ttgaagtaat ttagaaaatt cgtgaccata aaaaatatta
     1381 tcgcctaata ctaaagcact aggcgcgcca ttta
//
LOCUS       DAOQMB010000609         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75875_length_1413_cov_10.013991, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000609 DAOQMB010000000
VERSION     DAOQMB010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75875_length_1413_cov_10.013991"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..398
                     /locus_tag="PLY05_12520"
     CDS             201..398
                     /locus_tag="PLY05_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019673613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HNC04164.1"
                     /translation="MANKKFVRSREHNIIAGVMGGIADYLGWSPFKVRLAFVIISSVS
                     AAVPGILIYIILWFLMPLAEE"
     gene            403..1335
                     /locus_tag="PLY05_12525"
     CDS             403..1335
                     /locus_tag="PLY05_12525"
                     /inference="COORDINATES: protein motif:HMM:NF020481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HNC04165.1"
                     /translation="MNLPYYHPHVRNLAWILAAPSLLSFLPEFNQPLTVLDDQFWQQQ
                     YQAYLPRLQQLEDNPLILEQFLAEHNNHRLGYYFEYLLLFWLQDNQYHCFELIKHRAT
                     LFKGKITIGELDYLVRNKITGEIEHWEVAIKFYLGYPPLTDAYHWLGANDNDSLGRKL
                     HHLATKQFLADQYQDYKINKRCLLVKGRLFYPSTAKNLLACAHSPTVSCLSPDHLQGN
                     WIMYQEFINSPEAAQLQWRHVGKDEWLADQQPFKQLPLANAQHLPPLPTARAELFLGF
                     NAQLEEQARCFVCGVPRAIHLLASTPLSQRNDVP"
BASE COUNT          410 a          260 c          281 g          462 t
ORIGIN      
        1 aagatattgc tctaaaaatt gtggtataac tataaaaaga aattttaact taggacttac
       61 gtagttatcg cttatttgtt tacatttcaa tcgtttttaa cgattttatg cttacaaaat
      121 gcgcttgttt cgactttgct caacacaagc tttcggtgaa atgcgtaagt cctgaattgt
      181 tgtatttgag agtaaaaaca atggccaata aaaaatttgt gcgttcacgt gaacataaca
      241 tcattgcagg cgtgatggga ggaatagccg attatttagg ttggagtcct tttaaagttc
      301 gcttggcatt tgtgattatt tcatcagtca gtgcagccgt accaggtatt ttaatttata
      361 tcattttgtg gtttttaatg cctttagcgg aggagtaaac gcttgaattt gccttattac
      421 catccacacg tgcgcaattt agcatggatc ttagccgcac ctagtttatt aagtttttta
      481 cctgaattta atcaaccctt aaccgtgttg gatgatcaat tttggcagca acaatatcag
      541 gcgtatttac cacgtttgca gcaacttgaa gataatcccc ttattttaga acagtttttg
      601 gcggaacata acaatcatcg tttaggttat tactttgaat atttgttatt gttttggtta
      661 caagataacc aatatcactg ttttgagtta attaagcatc gagcaacgct gtttaaagga
      721 aaaattacta ttggcgaact ggattatttg gttagaaaca aaataacagg cgagattgaa
      781 cattgggaag tggccattaa attttattta ggttatccac cactgactga tgcctatcat
      841 tggttaggag caaacgataa cgactcttta ggacgaaaat tgcatcattt ggcgactaaa
      901 caatttttgg ctgatcaata tcaggactac aaaattaata aacgttgttt gctagttaaa
      961 ggacgattat tttatccttc aaccgctaaa aatctcttag catgcgcgca cagtccaacg
     1021 gtttcttgtt tatcacctga tcatttacaa ggcaattgga tcatgtatca agagtttatt
     1081 aacagtcccg aagccgcaca attgcagtgg cggcacgtcg gcaaagatga gtggttggcc
     1141 gaccaacagc cctttaaaca attgccattg gcaaacgctc aacatttgcc gccattgcca
     1201 accgctcgtg cagaattatt cttagggttt aatgctcaat tagaagaaca agcgcgttgt
     1261 tttgtgtgtg gtgtgccaag agctattcac ttgttggctt cgactccgct cagccaacgt
     1321 aacgatgtac cctgagcgca gtcgaagggt catgtttcag cggttttctg atgtactagc
     1381 caacaatttt tgttggctat acctacataa taa
//
LOCUS       DAOQMB010000610         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_75880_length_1413_cov_6.328424, whole genome shotgun sequence.
ACCESSION   DAOQMB010000610 DAOQMB010000000
VERSION     DAOQMB010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75880_length_1413_cov_6.328424"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLY05_12530"
     CDS             complement(<1..578)
                     /locus_tag="PLY05_12530"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNC04166.1"
                     /translation="MIKLIVLLCCFIFGAWIGNIFLADSGYVLIVWQQTSIELSLFLA
                     VLLLILTAGIGLVALELLLGVFGLRALLNKWLQQRRSLRAQRSYNKGLEALVLENFRR
                     AEKLFNQSAKASSEPLAAYLAAAQAAQGQQASQRAEDYLQLADSYQHRLTVQIMRIRL
                     WLQTGQWEAAAAQLKSIYPHYKKEPVVNQLLL"
     gene            complement(575..>1413)
                     /locus_tag="PLY05_12535"
     CDS             complement(575..>1413)
                     /locus_tag="PLY05_12535"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNC04167.1"
                     /translation="QSLKEQQVHYQSKSILLQQKVDSLESQLAQIKKQVQPLQKPYAL
                     IKQVQHYVDLAEQHLWLQHDSQSASQLLAIADELLQHYGNQQTLALRQAIAADQLLLS
                     QVQQVDVAGISLKIEALKLASQNLTLPPLKSPTLTSTQTIEVAQSAWEKGWAALQKLI
                     TIQHYNQSVTPLLTQEQRVLVQQNVYLALQQAQLALWAHQPQRYQQSLSNAEQLLMPY
                     QQLNLQYATAIKEVQNLKQLPMQESLVKLVNTPPVLVQLLNQQTEQEQTPMPDTMQDA
                     SL"
BASE COUNT          406 a          268 c          256 g          483 t
ORIGIN      
        1 tctaataata attgattaac aaccggctct tttttgtaat gtggataaat gctttttaat
       61 tgagcggctg cggcttccca ttgtcccgtt tgtaaccaca aacgaatgcg cataatttgc
      121 acagttaaac gatgttgata actatccgcc aactgtaaat aatcttctgc acgttgtgag
      181 gcttgttgtc cttgtgcggc ttgtgctgcg gctaaataag ccgctaaagg ctcgctactt
      241 gcctttgctg attggttaaa cagcttttca gcacgacgaa aattctctaa cactaaagct
      301 tctaaacctt tgttataact gcgctgggct cgcaaagaac gtctttgttg cagccattta
      361 ttcaataagg cacgtaaacc aaatacacct aacaataact ctaaggcaac aagtccaatt
      421 cctgctgtca atattaataa taaaacagct aaaaataagc ttaactcaat ggatgtttgc
      481 tgccaaacaa tcagtacata accactatct gctaaaaaga tattgccaat ccatgcacca
      541 aaaataaaac agcataataa aacaatgagt ttaatcataa actagcatcc tgcattgtat
      601 caggcatggg cgtctgttct tgctcagttt gttgatttaa taattgcact aatacaggtg
      661 gtgtattcac taatttaact aagctttcct gcataggtaa ttgttttaaa ttttggactt
      721 ctttaatagc agtggcgtat tgtagattta attgctgata aggcatcaat aattgctcag
      781 cattacttaa actctgctga taacgttgtg gttgatgtgc ccataaagct aattgtgcct
      841 gttgtaaggc caaatacaca ttttgttgca ccaaaacacg ctgctcttgc gttaataaag
      901 gggttactga ttgattataa tgttgaattg taatcagttt ttgtaacgct gcccaacctt
      961 tttcccacgc actttgggcg acttctattg tttgggttga tgtcagtgtc ggacttttta
     1021 gaggaggtaa agtcagattt tgacttgcta attttaatgc ttctattttt aaagaaatcc
     1081 ctgcaacatc aacttgttgt acttgactta ataacaattg atcagccgca attgcttgtc
     1141 ttaaagccag tgtttgctga ttaccataat gttgtaatag ttcatcggcg attgctaata
     1201 attggctggc actttggcta tcatgttgca accacaaatg ctgctctgcc aaatcaacat
     1261 aatgctgtac ttgtttaatt aaggcataag gcttttgtaa gggttgaact tgttttttaa
     1321 tttgtgctaa ttgcgattct aagctatcta ctttttgctg tagtaaaata ctcttacttt
     1381 gataatgtac ttgttgctcc ttaaggcttt gta
//
LOCUS       DAOQMB010000611         1412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_76003_length_1412_cov_5.229182, whole genome shotgun sequence.
ACCESSION   DAOQMB010000611 DAOQMB010000000
VERSION     DAOQMB010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76003_length_1412_cov_5.229182"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLY05_12540"
     CDS             <1..816
                     /locus_tag="PLY05_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNC04168.1"
                     /translation="YQRQNVQGRNEIHNRNTDFTQQQAREIVVHRFIDYWIATQSNLT
                     EQDVKDIKKALTYKSYHDGFVTFIGIAADIHDDVQYLKDLAKKVTPAEVKSYYQHHKQ
                     DFKRIEKVKAQHITVADEKTANMIIEQLKKGAKFADLAKQFSMAMDKNTGGDLGWVVH
                     NRNNPTWLSTLAFVQTVNVPSRPVRTPNGQWEILLITAKEEGYQPADSESVRYLASQV
                     IARQKMVKEYQTIRKNLIQQADIHISPILKQEMINLEKDVMIAKPSEEDHHHH"
     gene            943..>1412
                     /locus_tag="PLY05_12545"
     CDS             943..>1412
                     /locus_tag="PLY05_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04169.1"
                     /translation="MKKPILGFSVAVVALAGLAFIGQSMFVSDQTQDTHFSAMSQQNN
                     DTSIAPTLQTTQAPVNVTTPPAPQLTGTDSPLLPMSESTVDAASSMANAMKNGDPRMP
                     PVARSEPQEKATEAELADPKLYAQYEARQTMRLYKGYINAADTEIPRLQQDIAKA"
BASE COUNT          475 a          309 c          276 g          352 t
ORIGIN      
        1 tatcaacggc aaaatgttca aggccgtaac gaaattcata accgcaacac agatttcacc
       61 caacaacaag cccgcgaaat tgtcgttcat cgttttattg actattggat agcgactcaa
      121 tcaaatttaa cagaacaaga tgttaaagac atcaaaaaag cactcactta taaaagctat
      181 catgatggtt ttgtgacgtt tattggtatt gctgctgaca ttcatgatga tgtgcaatac
      241 ttaaaagatc tcgctaaaaa agtgacgcct gcggaagtca aaagctatta tcaacaccac
      301 aaacaagatt ttaaacgcat tgaaaaagtt aaggctcaac atatcaccgt tgctgatgaa
      361 aaaacggcta atatgattat tgagcaacta aaaaaaggcg caaaatttgc cgacttagcc
      421 aaacaatttt cgatggcgat ggataaaaac acaggcggcg atttaggttg ggtggtacac
      481 aatcgaaaca acccaacatg gttaagcact ttggcttttg tacaaaccgt gaatgtgcca
      541 tctcggcctg tgcgtacgcc caatggtcaa tgggagattt tattaattac cgcgaaagaa
      601 gaaggttacc aacctgctga tagtgagagt gtccgttatt tagcctctca ggtaattgca
      661 cgccaaaaaa tggtgaaaga ataccaaacc attcgcaaaa acttgattca acaagcagat
      721 attcatatta gtcctatact taaacaggaa atgattaact tggaaaagga cgtcatgatt
      781 gctaaaccta gcgaagaaga tcatcaccat cactaatcat aggacttacg catttagcca
      841 acattagcgt tttttgctcg ctcaacgttg ttaaaaacga ttgttgagcg agcaaagaag
      901 cgaaaactgc gtaagcccta ataaaagtcc gtgagagaca ttatgaaaaa acccattctt
      961 ggcttttccg ttgctgttgt tgctttagct ggccttgcat ttattggtca aagtatgttt
     1021 gtgagcgacc aaactcaaga cacgcatttt tctgctatgt cacaacaaaa taatgacaca
     1081 tccatcgcac caaccttgca gaccactcaa gcacctgtca acgtgacaac ccctcctgcc
     1141 ccacaactaa caggcacaga tagcccgtta ttaccaatgt ctgagtctac ggtagatgcg
     1201 gcaagttcaa tggcgaatgc gatgaaaaat ggtgatcctc ggatgccacc tgttgctcgc
     1261 tctgagccac aagaaaaggc cacagaagcg gaattggctg atcccaagct atatgcccaa
     1321 tacgaagcac gacaaactat gcgtttgtat aaaggctata ttaatgccgc agacaccgaa
     1381 attccgcgtt tacaacaaga tattgctaaa gc
//
LOCUS       DAOQMB010000612         1408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_76435_length_1408_cov_9.632668, whole genome shotgun sequence.
ACCESSION   DAOQMB010000612 DAOQMB010000000
VERSION     DAOQMB010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76435_length_1408_cov_9.632668"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="PLY05_12550"
     CDS             <1..1058
                     /locus_tag="PLY05_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HNC04170.1"
                     /translation="SALTRASENMLKALGAWSLIDKKRFAPFSSMYVREQQGHGELYF
                     HADDLGVSHLGCLLENRLLQWALTEQAVASPQVTLLAPDKVVALEKGITKWSVTLASG
                     IKIDTPLVVGADGAFSVVRQLAGIEIDTWDYQQQAIVCSVKTESPHQDCARQSFLTTG
                     PLALLPLSDPHYCSIVWSVQQDEAHKLMALSDDEFLQALERAFPCELGQMLSCDQRYS
                     FPLVARHAQRYCLEGLVLIGDAAHTIHPLAGQGVNLGLLDAAVLAEEIELALKKSLAI
                     SHLQVLSKYSRRRRSHNALTMHSITALERTYAATSTTMIMARNEGVRFVNKTAWLKEF
                     FEKQAMGLVGDLPQLAK"
     gene            1189..>1408
                     /locus_tag="PLY05_12555"
     CDS             1189..>1408
                     /locus_tag="PLY05_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04171.1"
                     /translation="MASNDYDDEFDETADPVDDEPEEDVATDAAAEPVEGEEGGKSKE
                     KDSDIIAVAETLTVSSKEAERRQLEEEME"
BASE COUNT          430 a          235 c          322 g          421 t
ORIGIN      
        1 tctccgcttt aacgcgtgct agtgaaaata tgctcaaagc cttgggtgca tggtcattaa
       61 tagataaaaa acgatttgca ccttttagca gcatgtatgt ccgtgaacaa caaggacatg
      121 gtgagttata ttttcatgcc gatgatttag gtgtgagtca tcttggttgt ttattagaaa
      181 atcgtttatt gcaatgggca ttaaccgaac aggccgttgc tagcccacaa gtcaccttgt
      241 tagctcctga taaagttgtg gcattagaaa aaggcattac taagtggtct gtgactttag
      301 caagtggaat aaaaattgat acgccattag tggtgggtgc tgatggtgca ttttcagtgg
      361 tgcgtcaatt agcgggtatt gagatagata catgggatta tcaacaacaa gcgattgttt
      421 gttcggttaa aacagaaagt ccacatcaag attgtgcgcg ccaaagtttt ttaactacag
      481 ggcctttagc tttattacca ttatccgatc ctcattattg ttctattgtt tggtcggtac
      541 aacaggatga agcacataag ttaatggctt taagcgatga tgagttttta caagctttag
      601 agcgtgcttt tccatgtgag ttaggacaaa tgctcagttg tgaccaacgt tatagctttc
      661 ctttagttgc acgtcatgca caacgatatt gtttagaagg tttggtgtta attggtgatg
      721 ctgcccatac tattcatcca ttagcaggac aaggcgtaaa cttaggttta ttagatgctg
      781 ctgttttggc ggaagaaata gagttagctc taaaaaaatc tttagccatt agccatctgc
      841 aagtattaag taaatatagt cgtcgtcgtc gctcacataa tgccttaacc atgcacagta
      901 tcacggcctt agaacgtact tatgcagcaa catcaaccac gatgattatg gctcgtaatg
      961 aaggcgtacg ttttgttaat aaaacggctt ggttaaaaga attttttgaa aaacaagcaa
     1021 tgggtttggt tggtgatttg ccgcaattag ctaaataata aacaattgca ctttttgttg
     1081 atttgctaaa gagtttgagg ctagaatttt ttttcaaaaa aggtaagaat ttggcattat
     1141 atgtaaaaga ttgtaataac ccctaaatcc ctgataacgg agatgataat ggctagcaat
     1201 gattatgatg atgaatttga tgaaacggct gatcctgtcg atgatgaacc agaagaagat
     1261 gtcgctacag atgctgctgc tgaacctgtc gaaggcgaag aaggcggcaa aagcaaagaa
     1321 aaagacagcg atattattgc agtagccgaa accttaaccg tctctagtaa ggaagcagaa
     1381 cgtcggcaac tagaggaaga aatggagc
//
LOCUS       DAOQMB010000613         1406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_76665_length_1406_cov_9.749075, whole genome shotgun sequence.
ACCESSION   DAOQMB010000613 DAOQMB010000000
VERSION     DAOQMB010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1406
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76665_length_1406_cov_9.749075"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PLY05_12560"
     CDS             <1..678
                     /locus_tag="PLY05_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004805995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HNC04172.1"
                     /translation="SDSRTNAGVDHIAVFRKLHLFGVEGERVLVLQSAGNLATSQAII
                     TLLRQRIQQQEEPNLHTVKTMYEAADLVGHTLREVMDKYKEQNMDFSCSLLLGGQILG
                     EEPKLYNIYPQGNFIAATQDTPYFQIGESKYGKPILDRALNYFSPIEKALRCALISFD
                     STIRSNLSVATPLDLMVYNNHSFKLPQGKRLYDDDPYFQAIRSQWSEGLQKVLAQLPP
                     PPAEYWL"
     gene            692..>1406
                     /locus_tag="PLY05_12565"
     CDS             692..>1406
                     /locus_tag="PLY05_12565"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNC04173.1"
                     /translation="MSIHVALNHVTHYRYDRLINLGPQVIRLRPAPHCRTRILSYSLK
                     VSPKQHFINWQQDPQSNYLARLVFPEKTQEFKVEVDLVAEMSVINPFDFFLEPYAENY
                     PFSYDASLDQELAPYLVKLPTTPVFDAYLQGISREKVRSIDFLVALNSALSQHIAYVI
                     RLEAGVQTPEETLTKKTGSCRDSAWLLVQLLRYLGFASRFVSGYLIQLTPDIKALDGP
                     TGTDVDFTDLHAWCEVYLPG"
BASE COUNT          411 a          255 c          285 g          455 t
ORIGIN      
        1 agtgattcac gcactaatgc cggggtagat catattgccg tgtttcgtaa attgcattta
       61 tttggtgtgg agggtgagcg cgttttagtg ttacaaagtg cgggcaattt ggcaacttca
      121 caagcgatta ttactttatt acgacagcgt attcagcaac aagaagaacc caacttacat
      181 accgtaaaaa cgatgtatga agcggctgat ttggtagggc ataccttacg cgaggtaatg
      241 gacaaatata aagagcagaa tatggatttt tcttgctctt tgttgttggg tgggcaaatt
      301 ttgggtgaag aacctaagtt atataatatt tatcctcaag gtaattttat tgccgcaact
      361 caagacacac cgtattttca aattggtgaa agtaaatatg gtaagcctat tttagataga
      421 gccttaaatt attttagtcc gatagaaaag gcattacgtt gtgccttaat ttcttttgat
      481 tcgactattc gtagtaattt atcagtagca acaccattag atttaatggt ttataacaat
      541 catagtttta agctgcctca aggtaaacgc ttgtatgacg atgacccata ctttcaagca
      601 attcgtagtc aatggtcaga aggcttgcaa aaagtcttag cacaattacc gccaccccct
      661 gccgaatatt ggctatagga gcaagcagat tatgtctatt catgtcgcac tgaatcatgt
      721 gacccattat cgttatgatc gtttaatcaa tttagggcca caagtcatta ggttacgtcc
      781 tgcaccacat tgtcgcaccc gaattttatc gtattcgtta aaagtatcac ccaaacagca
      841 ttttattaat tggcaacaag acccacaaag taattattta gcaaggttgg tttttcccga
      901 aaaaactcaa gagtttaaag tagaagtcga tttagtggcc gagatgtcgg tgattaatcc
      961 ttttgacttt tttttagagc cgtatgccga aaattatccc tttagctatg atgctagttt
     1021 agaccaagag cttgcccctt atttagtaaa actgccaaca acccccgtat ttgatgcgta
     1081 tttacaaggt attagtcggg aaaaagttcg ttcaattgat tttttagtgg ctttaaatag
     1141 tgcattatca caacatattg cttatgtgat tcgtttagaa gcaggagtgc aaacacccga
     1201 agaaacgctc actaaaaaaa caggttcttg ccgtgactct gcatggttat tagtccagtt
     1261 attacgttat ttaggttttg cttctcgttt tgtttcgggt tacttaattc aattaacgcc
     1321 tgatatcaaa gcacttgatg ggccaacagg tacagatgtt gatttcaccg atttacatgc
     1381 ttggtgtgaa gtgtatttac cgggtg
//
LOCUS       DAOQMB010000614         1403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_76981_length_1403_cov_9.663205, whole genome shotgun sequence.
ACCESSION   DAOQMB010000614 DAOQMB010000000
VERSION     DAOQMB010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76981_length_1403_cov_9.663205"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..1247
                     /locus_tag="PLY05_12570"
     CDS             327..1247
                     /locus_tag="PLY05_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04174.1"
                     /translation="MENLRTQALKGFSVSMLALAMVACGGGSSDDPAPAANNNTNNNT
                     ASSLTAVELAKVASAIQSAKTIFDVVSYGGIDGIEEVITDGKAGKDINCGTSLSAGKF
                     KVTANTATAYSKDGSAVNDLCLESGEKWDGSIAYECKDAACDNSVVTSTNAVWGDTVN
                     NVDLKISGTMTTSTNGDVFKGTSSISKSGQSTLFTFSDVGLTQSYPAKNTVGGSGALS
                     ISNGSATNCIDGTYSYTVATNLTMPSGTQRLNGGSISIKSGSNDLGTVSFNTDGSIKV
                     KLPSGVESTVSKLDFEGTCGLKEAYMFSEK"
BASE COUNT          429 a          211 c          312 g          451 t
ORIGIN      
        1 tttcatttaa aggcgaattt tagttttctt ttttaaagcg tcaggttatt ttgttggtgt
       61 tgggctgcaa ttcatcgttg attaatgctg ggaagtgctt tgcaagttct aactaatcat
      121 tcaactggac acaaccccga ccgattatgg tgatattttt taggtttttg gcaaactctc
      181 acttgtttac tttgctgtgg ggtcgtgcca gttaatttta agttagacag ttaaaaactc
      241 tttaactaaa ggaataacag gaaaaccctc actgtttaag ccgatattag acgttctttt
      301 aatcgaggga aaattaagga agtgtcatgg aaaatctaag aactcaagcc ttgaaaggtt
      361 ttagtgtatc aatgttagcg ttagcaatgg tcgcttgtgg tggtggaagt agcgatgacc
      421 ctgcacctgc tgcgaataat aatactaaca acaacacagc ttcatcatta acggcagttg
      481 agttagcaaa agtagcctct gctatccagt cagctaaaac aatttttgat gttgtttcat
      541 atggtggtat tgatggaatc gaagaagtta taacagacgg aaaagctggt aaagatatta
      601 attgtggaac aagtttgagt gctggtaaat ttaaggtcac tgctaatacc gcaacagctt
      661 atagcaaaga tggaagtgct gtaaacgatt tatgtctaga aagtggtgaa aaatgggatg
      721 gaagtattgc ctatgaatgc aaagatgctg cttgtgataa tagtgtcgta acatcaacaa
      781 atgctgtatg gggtgatact gtaaataatg tagatttgaa aattagtggc acaatgacta
      841 catcaactaa tggtgatgtc tttaagggaa catcatcaat tagtaaaagt ggtcaaagta
      901 cactgtttac ttttagtgat gttggtttaa ctcagagcta tcctgcgaaa aatacagttg
      961 gaggttctgg cgcattaagt atttcaaatg gttcggccac aaattgcatt gatggtactt
     1021 attcttatac cgttgctact aatttaacaa tgccttcagg tacgcaaaga ctcaatggtg
     1081 gcagtatatc aattaaaagc ggcagtaatg atttaggcac tgtatcattt aatactgatg
     1141 gtagtatcaa agtaaagctg ccatcaggtg ttgaaagcac agtttctaaa ttagattttg
     1201 aagggacttg tggcttaaaa gaagcctata tgttctctga aaaataaaaa gtttgggttg
     1261 ttgtctaact tggcactcaa cactgacaac aacccgcgct ggatttgagt tgttttttac
     1321 atttttagca aacttgctca tgcccaaatt gttgtgggtt gttgcaggtt agtgcaatag
     1381 ttagaagttc tttgggttaa gtg
//
LOCUS       DAOQMB010000615         1398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_77524_length_1398_cov_14.431869, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000615 DAOQMB010000000
VERSION     DAOQMB010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77524_length_1398_cov_14.431869"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PLY05_12575"
     CDS             <1..195
                     /locus_tag="PLY05_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC04175.1"
                     /translation="MTDGVSDAFFDSDNALKNAKAWQYLWQFLQQQNALADENALLAC
                     LDFWSKGNHDDRTLVAILEQ"
     gene            197..>1398
                     /locus_tag="PLY05_12580"
     CDS             197..>1398
                     /locus_tag="PLY05_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HNC04176.1"
                     /translation="MANVVRLTASDGSVVEFFDEIKAAGAMKDVYFSPDKRYVVALFR
                     KKNDQALKERLQLITGQYRERIFNQQGGDYWRSVYCWPTAMVEYQGKIGVVMPFYASH
                     FFFDYGSKQNDMLGIKGKEKEGKWFASPSNRQKFLDSRELGNWLHHLRICLQIARATR
                     RLHAAGLAHSDLSYKNVLVDPITGSACLIDLDGLVVPDKFAPDVVGTPDFIAPECVKT
                     AHLERNDPKRVLPSIRTDEHALAVLIYMYLLYRHPLRGDKVHDMKDSQRDESLMMGEN
                     ALFIEHPTDSSNRINVAYVKASELPWKDTQKLPYTLTGSYLSPLFECAFIDGLHQPYV
                     RPSADEWEHALVKTVDLIQPCINPDCEQKWYVFANSTKPICPFCQTPFVGQLPVLNLY
                     SASQEGRF"
BASE COUNT          395 a          243 c          316 g          444 t
ORIGIN      
        1 atgaccgatg gcgtcagtga tgcgtttttt gactcagata atgccttaaa aaatgcgaaa
       61 gcatggcaat atttatggca gtttttacaa caacagaacg ccttagcgga tgaaaatgcc
      121 ttattagcgt gcttagattt ttggtcaaaa ggcaatcacg atgaccgtac tttagtggca
      181 attttggaac aataacatgg ctaatgtagt gcgtttaact gcttctgatg gttcagtggt
      241 cgagtttttt gatgaaatca aagcggctgg cgcaatgaag gatgtgtatt tttcgcccga
      301 taaacgttat gttgtggctt tgtttagaaa gaagaatgac caagctttaa aagagcgttt
      361 gcaactgatt acaggtcaat atcgagagcg gatttttaat caacaaggcg gtgattattg
      421 gcgtagtgtg tattgttggc caacagcaat ggtggagtat caaggcaaaa ttggtgtcgt
      481 gatgcctttt tatgccagcc atttcttttt tgattatggt tctaaacaaa acgatatgct
      541 cggtattaaa ggcaaagaaa aagaaggcaa atggtttgct tcaccatcaa atcgacaaaa
      601 atttttggat agtagagagt tagggaattg gttgcatcat ttacgcattt gtttgcaaat
      661 tgcccgtgct acgcgccgtt tacatgctgc aggtttagct cattccgatt tgtcatataa
      721 aaacgtcttg gttgatccca ttacaggcag tgcctgttta attgatttag atggtttggt
      781 tgtgcctgat aaatttgcac ctgatgtggt gggaacgcct gattttattg cacctgagtg
      841 tgttaaaacc gcacatttag agcgtaatga ccccaaacgt gtattaccca gtattcgtac
      901 cgatgaacac gctttggcag tattgattta tatgtacttg ttatatcgcc atcctttacg
      961 tggtgacaaa gtacatgata tgaaagattc gcaacgtgat gaaagtttga tgatgggcga
     1021 aaatgcttta tttattgaac atcccacaga cagcagcaat cggattaatg ttgcttatgt
     1081 caaagcctca gaattaccgt ggaaagatac gcaaaagtta ccttataccc taacaggtag
     1141 ttatttaagt ccattatttg agtgtgcatt tattgatggt ttgcatcaac cctatgtccg
     1201 gcctagtgcg gatgaatggg aacatgcttt ggtaaaaacc gttgatttaa ttcagccttg
     1261 tattaatcct gactgcgaac aaaaatggta tgtatttgct aatagcacca agccgatttg
     1321 tccgttttgc caaacgccct ttgttggcca attacctgtg ttaaatttgt actctgccag
     1381 ccaagaagga cgattttt
//
LOCUS       DAOQMB010000616         1388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_78546_length_1388_cov_10.692423, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000616 DAOQMB010000000
VERSION     DAOQMB010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78546_length_1388_cov_10.692423"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..520
                     /gene="pgaD"
                     /locus_tag="PLY05_12585"
     CDS             92..520
                     /gene="pgaD"
                     /locus_tag="PLY05_12585"
                     /inference="COORDINATES: protein motif:HMM:TIGR03940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HNC04177.1"
                     /translation="MHNTHLIINARSHLPWQTKVLSDSLTAIMWGAWLFLWRPVVHVV
                     IVLHAIGCTDVGQISSMLHSCTPDINFENGLLALLTSVTALLLWSLLPSKKVITAHRV
                     NSLQDYAQYFQLNDQQINQGRHQQIAVVYHNEQGQIIGIK"
     gene            655..>1388
                     /locus_tag="PLY05_12590"
     CDS             655..>1388
                     /locus_tag="PLY05_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004878498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC04178.1"
                     /translation="MSDNTQTITIDGKQRYYEAAPTDIDVDNFRKVVTSRRSVRKFTA
                     KPIPKAVLDDCLDMALLAPCSSGLQQWEFYVVRSADKKAKLVKACMSQLAAKTAAELI
                     VCVARTDRVDEFSKRMLREWPMPDVPPLVKRYYDLIPYNYSSGPLNSFAYAKKAAFAL
                     GGLVAAVPRGPYTKSEVELWAAKSTALACENLVLAFRAHGFDTCMMEGFDAARVSKLL
                     NLQDGAFPIMVIGAGERADDGVFWPQV"
BASE COUNT          409 a          279 c          272 g          428 t
ORIGIN      
        1 ggtaataaga tttgataagg acagctttta agtgcttgat atatttatct cgcatgatta
       61 ttccaatcaa tgtccataca ggtcatttgt tatgcacaat actcacttaa ttattaatgc
      121 acgttcacat ttaccttggc aaactaaagt cttatctgac tctttaaccg ctattatgtg
      181 gggagcatgg ctttttttat ggcgaccagt agtacatgtt gttattgtat tacatgctat
      241 tggctgcaca gatgtaggcc aaattagctc tatgctccat agctgcacac ccgatattaa
      301 ctttgaaaat ggcttattag cactactcac ttctgtcacg gcgttgttat tatggtcgct
      361 actaccatca aaaaaagtta taactgcaca tcgtgttaat agcttgcaag actacgcaca
      421 atattttcaa ctaaatgacc aacaaattaa tcaaggccgt caccaacaaa ttgcagtggt
      481 ttatcataat gagcaaggcc aaattattgg tatcaaataa gctccttaca caccaaagct
      541 attgaaatac attcaatagc ctgtagattc ttcccaaaaa aacagtttga catttatcaa
      601 accatctaat aatctcagtc tatacctact gcataaatag attgaggttt agcaatgtct
      661 gataatacac aaacgattac gattgatggc aaacagcgtt attacgaagc agcacccacc
      721 gatattgatg ttgataactt tcgtaaagtc gtcactagcc gtcgttcggt acgcaaattt
      781 actgccaaac ccatccctaa agctgtgctt gatgactgtt tagatatggc tttactcgcc
      841 ccttgttcgt caggtttaca acaatgggag ttttatgtag tgcgctcagc tgataaaaaa
      901 gccaaattag tcaaagcgtg tatgagtcaa ctagctgcca aaactgccgc tgaattgatt
      961 gtctgcgtag cgcgtactga ccgtgttgat gaattttcta aacgtatgtt gcgcgaatgg
     1021 ccaatgcctg atgtgcctcc attggtcaaa cgttattatg acttaattcc ttacaattat
     1081 tcatctggcc cattaaatag ttttgcctat gccaaaaaag cagcttttgc tttaggtgga
     1141 ttagtggctg ctgtgccaag aggcccatat accaaaagtg aagtcgaatt atgggcagcc
     1201 aaaagcactg cccttgcgtg tgaaaactta gtgttagcgt ttagagcaca tggttttgat
     1261 acctgtatga tggaaggctt tgatgcggct cgagtaagca aattattaaa cttacaagat
     1321 ggtgcattcc caattatggt gattggtgca ggtgaacgtg cggatgatgg cgttttttgg
     1381 ccacaagt
//
LOCUS       DAOQMB010000617         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_79474_length_1380_cov_16.137358, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000617 DAOQMB010000000
VERSION     DAOQMB010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79474_length_1380_cov_16.137358"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="PLY05_12595"
     CDS             complement(<1..536)
                     /locus_tag="PLY05_12595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNC04179.1"
                     /translation="MTNKNIYLVPLLITSSFLLTACEKPSVAADKPRLVKLISVQTSS
                     SQQSLDLAGEIKARVESPLGFRVAGKIVRRLVETGQTVHKGQALAELDPRDYELAKTA
                     STSQLTAAKSDLALAEAEYKRFQELQTKQFVSELDLDRKRVAVSAAAARLKSLESNVS
                     LDSNRIADTILRADSDGVV"
     gene            complement(526..1209)
                     /locus_tag="PLY05_12600"
     CDS             complement(526..1209)
                     /locus_tag="PLY05_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNC04180.1"
                     /translation="MNFCPRSRGRPRFQQAALSREQIIERAFIAFAEQGYEQVSLRLL
                     ANHCGISDSLLHHHFGSKQQLWQEAADNHIAPLMATLTSNLEALAANDRPAQALRHNL
                     PQSLKNMVSKPAALAFIFREGDLNNERSEYLRQRYMRPYLNRLDALFNQAVLDGDFRP
                     IPPAARHVLIMGFLRSIVIPAHLQDELAPHLSQPERTNALIDDIAQLLLHGFSQSSPP
                     LLGAITHDQ"
BASE COUNT          396 a          275 c          318 g          391 t
ORIGIN      
        1 accacaccat cactgtcagc gcgtaaaatg gtatcagcaa tgcgattgct atctaaagaa
       61 acattacttt ctaaactttt taagcgtgcg gcggcggcag acacagccac acgtttgcgg
      121 tctaaatcta actccgagac aaactgtttg gtttgtaact cttgaaaacg tttgtattcg
      181 gcttcagcta aagctaaatc cgatttagcc gccgttaatt gtgaagtact cgcggttttg
      241 gcgagttcgt aatcacgcgg gtcaagttcg gctaaggctt ggcctttgtg tacagtttgg
      301 ccggtttcca ccaaacgacg aacaatttta cctgcaaccc taaagcctaa tggcgattca
      361 acacgtgctt taatttcgcc tgctaaatca aggctttgtt ggctggagct agtttgcacg
      421 ctaatcagtt tgactaagcg tggtttatca gcagcgactg atggcttttc gcaggccgtt
      481 aacagaaaac tgctggtgat taataagggc actaaataga tgtttttatt ggtcatgagt
      541 gatcgctcct aaaagcggtg gcgaggattg gctgaaaccg tgtaataaca actgggcaat
      601 atcatcaatc aaagcattgg ttcgctctgg ctgacttaaa tgtggggcaa gttcgtcttg
      661 taaatgagca gggattacta ttgagcgcaa aaagcccata atcaacacat gacgagccgc
      721 aggcggaata ggacgaaaat cgccatctaa caccgcttga ttaaataagg catctaaacg
      781 attgagatac gggcgcatat aacgttggcg taaatattcc gaacgctcat tgtttaaatc
      841 cccctctcga aaaataaagg ctaaggccgc aggtttgctg accatatttt ttagcgattg
      901 aggtaagttg tgtcgtaagg cttgggcagg acggtcgtta gccgctaagg cttctaggtt
      961 actggttaaa gtagccatta acggggcaat atgattatcg gcagcttctt gccaaagttg
     1021 ctgttttgag ccaaagtgat ggtgtaataa gctgtcacta atcccacaat gatttgctaa
     1081 taagcgcaaa gacacttgtt cgtaaccttg ttcggcaaag gcaataaagg cgcgctcaat
     1141 aatttgttca cgcgataaag cagcttgttg aaagcgtggt cttccacggc tacgaggaca
     1201 aaagttcata gtaaaatatt taattggtca gttgcccaat taaatagcat agcttttgag
     1261 ctttgtcagc tttacaatgt caagattaac catttttttt acaaaatgat gtatattagg
     1321 caaaaaatca ggctaatgtt aaaggttaac taggcttcct tgatttttgt gataggaaaa
//
LOCUS       DAOQMB010000618         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_79584_length_1379_cov_14.246979, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000618 DAOQMB010000000
VERSION     DAOQMB010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79584_length_1379_cov_14.246979"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1379
                     /locus_tag="PLY05_12605"
     CDS             37..>1379
                     /locus_tag="PLY05_12605"
                     /inference="COORDINATES: protein motif:HMM:NF014722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HNC04181.1"
                     /translation="MATLQTLNFDNSFARLASCLFTPVKPQALVQPFFIHANPQVAKL
                     LELDYSAEELVRYFSGAAPLPNSQPLAMTYSGHQFGMYNPQLGDGRGLLLGEVVNQQG
                     ERWDIHLKGAGKTPYSRFGDGRAVLRSSIREYLCGEAMHGLGIRTTRALCLIGSKEPV
                     QREKIETAATIVRVADSHIRFGHFEWLFHNQEKLLPSFADYVINRHYPECRQADKPYA
                     ALLTAIVERTATMIANWQLVGFAHGVMNTDNFSITGSTFDYGPYGFLDSYQPSFICNH
                     SDTSGRYAWNQQPSIGLWNLNALAYALSPLIAKNDIIDALQNYEKVLLAHYYQGIKAK
                     LGLTIDHGDDNNLAFDFLDILMKNHMDYTRSFRALSYSGKADKFCALRDDCIDRERFD
                     NWFKQYQQRLSLETQSDEQRQQQMQAVNPKYILRNYLAQIAIDKAEQGDYSEIDI"
BASE COUNT          388 a          281 c          284 g          426 t
ORIGIN      
        1 tttatgatgc aagcaagatt ttcgtgagca tcatttatgg cgactttaca aaccctgaat
       61 tttgacaata gctttgctcg tttagccagt tgtttattta cgcctgttaa accgcaagcc
      121 cttgtccaac cattttttat tcatgctaat ccccaagtcg ccaaactgtt agagctagat
      181 tacagcgcag aagaattagt ccgttatttt agtggtgcag cccccttacc caatagccaa
      241 ccgttggcca tgacctatag cggccaccag tttggtatgt acaacccaca attgggtgat
      301 ggtcgaggtt tattattggg cgaagttgtc aatcaacagg gtgagcgttg ggacattcat
      361 ctcaaaggcg caggcaaaac gccctattca cgttttggtg atggtcgtgc ggtgttacgt
      421 tcgtcgattc gtgaatattt atgtggcgaa gcaatgcatg gtttaggcat tcgcactact
      481 cgcgccttat gtttaattgg ttctaaagaa cctgtccaac gtgaaaaaat cgaaactgct
      541 gccactattg ttcgtgttgc cgatagtcat attcgttttg gtcattttga atggttattt
      601 cacaatcaag aaaagctgtt accctcgttt gctgattatg tcattaatcg ccactaccca
      661 gaatgtcgtc aggccgacaa gccctatgct gctctattaa ccgcgattgt tgagcgcacc
      721 gccaccatga ttgccaattg gcaattagtg ggttttgcac atggcgtcat gaatacggat
      781 aatttttcga ttacaggcag cacctttgat tatgggcctt atggcttttt ggatagctat
      841 caaccgagtt ttatttgtaa tcactctgat acgtctggcc gttacgcatg gaatcaacaa
      901 cccagtattg gcttatggaa cttaaatgcc ttggcttatg ctttatcacc attgattgcc
      961 aaaaatgaca ttattgatgc cttacaaaac tatgaaaaag tgctattagc tcattattat
     1021 caaggcatta aagctaaatt aggtttaacg atagatcatg gtgatgacaa taacttagcc
     1081 tttgattttt tagacatttt aatgaaaaac cacatggact acacacgcag ctttagggct
     1141 ttaagttaca gtggtaaagc agataaattt tgtgctttac gtgacgattg tatagaccga
     1201 gagcgttttg ataactggtt taaacagtat caacagcgtt taagcctaga aacacaaagc
     1261 gatgaacaac gccaacaaca gatgcaagcc gttaatccta aatacatttt gcgtaattat
     1321 ttggcacaaa ttgccattga taaagctgaa caaggtgact acagcgaaat tgatatttt
//
LOCUS       DAOQMB010000619         1379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_79588_length_1379_cov_8.027190, whole genome shotgun sequence.
ACCESSION   DAOQMB010000619 DAOQMB010000000
VERSION     DAOQMB010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1379
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79588_length_1379_cov_8.027190"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..157)
                     /locus_tag="PLY05_12610"
                     /pseudo
     CDS             complement(1..157)
                     /locus_tag="PLY05_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730639.1"
                     /note="catalyzes the formation of 8-amino-7-oxononanoate
                     from 6-carboxyhexanoyl-CoA and L-alanine; incomplete;
                     partial in the middle of a contig; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
     gene            complement(271..1104)
                     /locus_tag="PLY05_12615"
     CDS             complement(271..1104)
                     /locus_tag="PLY05_12615"
                     /inference="COORDINATES: protein motif:HMM:NF019227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin-type HINT domain-containing
                     protein"
                     /protein_id="HNC04182.1"
                     /translation="MSKEVSASGFVAGTLVHTDKGLVPIEQLKVGDMVLSKHESGEGE
                     QAYKRVVNTFKSAEKLPIVSVSFNDLSNRLGSMRLFCTDNHPFWNPISKEWTPAKDLR
                     FNSHVVLYDLNDKIVGLDDDAGYLVKTAHPQVALCSYLYHVGRRSNFGMLVDFRQNNP
                     LLVIGKEGFSEFEFGWYGMNWDRSLHPDENTKVLSEFEHPDTQFYFNAYDESERRPSI
                     IDAYDDPDSVNYMYTDYVYNIEVEDFHTYYVGKAGIWVHNSNTCLNLGVFPSTPKVTQ
                     P"
     gene            complement(1234..1379)
                     /locus_tag="PLY05_12620"
                     /pseudo
     CDS             complement(1234..1379)
                     /locus_tag="PLY05_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="biotin synthase BioB"
BASE COUNT          418 a          300 c          254 g          407 t
ORIGIN      
        1 cttcaagggc ttgatgttgg cgatgatgac cacaaaccaa atgtgctgca ccactgccga
       61 caccatcttg ctcggctgat tgaataaagg cttttttgat tacaggatga ttggctaaac
      121 ctaaataatc attactcgag aaattgaggt aagtttggcc ttgatgaatt aacgtcgcac
      181 cttgaggaga gtcatgggtt ttgcattgcc gaaaacgctg ttgttgatgg cgttcgagga
      241 gtttggtggt gaggttgaag ttggtaaggg tcatggctga gtaacttttg gagtgcttgg
      301 aaaaactcca agattcaaac aagtatttga gttatgtacc catatgccag ctttacccac
      361 atagtaggta tgaaaatctt caacttcaat gttataaaca taatctgtat acatgtagtt
      421 gactgaatct ggatcgtcgt aggcatcaat gatactaggt cttctttcgc tttcgtcata
      481 agcattaaaa tagaattgcg tatcaggatg ctcaaactct gataatacct ttgtattttc
      541 atcaggatgc aatgacctat cccaattcat accataccag ccaaactcga actctgaaaa
      601 tccctctttt cctatcacca gtagagggtt gttttgtcta aaatcaacca gcattccaaa
      661 attactacga cgcccgacat gataaagata actacaaaga gcaacttgag ggtgtgctgt
      721 tttaactaaa tagccagcat catcatccaa tcctactatt ttatcgttta aatcataaag
      781 tactacgtga ctgttaaatc tcaaatcttt tgcgggtgtc cattctttgc tgatagggtt
      841 ccaaaaagga tgattatctg tacaaaaaag acgcatactt cctaaacgat ttgataaatc
      901 attaaaagac acgctcacaa taggtaattt ttccgccgat ttaaacgtgt ttaccactcg
      961 tttataagcc tgttcacctt caccactttc gtgctttgac aacaccatat caccaacttt
     1021 aagttgctca atggggacta aacctttatc agtatgcact aacgtccctg ccacaaaacc
     1081 cgacgctgaa acctctttag acatgatgat acccagaaga aatactaata aagcagtgaa
     1141 gtccctaaat cacaccgcca tcgcatcttt ccacaaggtc tttttggcaa caggcaaact
     1201 atctgccacg gttgtttcca cttgcaaggc attaataccc aatttttcaa acaacaaacg
     1261 gtcggcatgg gtttcggggt tactggtggt taacagcttt tcaccataaa aaatcgaatt
     1321 tgcaccagca aaaaaacata aggcctgtaa ttgctcattc attttttcac gacctgccg
//
LOCUS       DAOQMB010000620         1376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_79980_length_1376_cov_4.180167, whole genome shotgun sequence.
ACCESSION   DAOQMB010000620 DAOQMB010000000
VERSION     DAOQMB010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79980_length_1376_cov_4.180167"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..317
                     /locus_tag="PLY05_12625"
     CDS             135..317
                     /locus_tag="PLY05_12625"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC04183.1"
                     /translation="MSNFPEIGYTPENLKHLIKLKGLTQMQVAVLMGVNHSTVARWLN
                     GSVTMPHEKWLKLLAA"
     gene            418..>1376
                     /locus_tag="PLY05_12630"
     CDS             418..>1376
                     /locus_tag="PLY05_12630"
                     /inference="COORDINATES: protein motif:HMM:NF037528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04184.1"
                     /translation="MSAITPILKAIRIALDAPTDENIFALADVAGLTIKKEVPNKIEV
                     VEHTTKKGKVIRGVVRTDLSYSEAKAIDEFTFKKDGGWFIREKHLTNEPILAGGDTPT
                     DNKPQAAVIESVQTDNLVNDADNNIEGLTRKFKPAAKKYKNPEIRLEPKEGAKLLKQT
                     LTKLFPNTKFKVNMSRGTAYGSVSVSWVDGASYDLVDVIAQHFKGSGFDGMSDSTYYL
                     NAINEDGKVVDYGVGYVSLSRGYSREFLEKLQSSFSDMLQNMIKQEGVTIKGSDFNAY
                     FDAKERWTQDKINEAKISHSTGKYHYGAIPEALNDDFELSSQS"
BASE COUNT          453 a          261 c          296 g          366 t
ORIGIN      
        1 agcggagtat ggacttcaag ataaacaaaa gccccgatat gcagtcgggg ctttttttat
       61 gctggctatg tgccagtaaa cattacttag tgactaatca caaagtaaaa acattctaac
      121 caaggcttac actcatgtca aactttcccg aaattggcta cacgcctgaa aacctaaagc
      181 atttgattaa gctaaaaggt ttaactcaaa tgcaagtggc tgttttaatg ggggtaaacc
      241 actcaaccgt tgcaagatgg ttgaatggta gcgtaaccat gccacacgaa aagtggctta
      301 aactattagc cgcctagtgc ggcttttttg tttgtatttc ctcctgtaac cactcacaaa
      361 acacatccta aaactgattt acacttactc aagtttaaac tttgcctgag taagattatg
      421 tctgctatca ctcctattct taaagctatt cgtattgccc ttgatgcgcc tactgatgaa
      481 aacatttttg cgttggcaga tgttgctggt ttaaccatca aaaaagaagt ccctaacaaa
      541 atcgaagtcg tggaacacac aaccaaaaaa ggcaaggtta tccgtggtgt agtccgtaca
      601 gatttaagct atagcgaagc taaagccatt gatgaattta catttaaaaa ggatggtggt
      661 tggtttattc gtgaaaagca tttaactaat gagccaattt tagcaggggg tgacacgccc
      721 acagataata agcctcaagc ggctgttatt gaatcagtgc aaacagataa ccttgttaat
      781 gatgcagaca ataacatcga gggattgacg cgcaaattta agccagccgc caaaaagtac
      841 aaaaaccccg aaattagact agagccgaaa gagggcgcaa aactactcaa gcagacatta
      901 acaaagttat tccccaatac taagtttaag gtcaatatga gccgtggcac agcttatggc
      961 agtgtaagcg tgtcatgggt ggatggcgcg tcttatgact tggttgacgt gattgcacag
     1021 cactttaagg gtagtggttt tgacggcatg agcgattcaa cttactatct aaatgcgata
     1081 aatgaagatg gaaaagtggt tgattatggc gtaggttacg ttagcctaag ccgaggctat
     1141 agccgtgaat ttttagaaaa gctgcaatca tcattttctg atatgttgca gaacatgatt
     1201 aagcaagaag gcgtgaccat taagggcagt gattttaacg cttattttga tgccaaagag
     1261 cgttggacac aagataaaat caatgaagct aaaataagtc atagcacagg aaaatatcat
     1321 tacggtgcga taccagaagc gttaaatgac gactttgaat tatcaagcca aagtga
//
LOCUS       DAOQMB010000621         1374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_80178_length_1374_cov_19.798332, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000621 DAOQMB010000000
VERSION     DAOQMB010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80178_length_1374_cov_19.798332"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..353)
                     /locus_tag="PLY05_12635"
     CDS             complement(141..353)
                     /locus_tag="PLY05_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003670252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HNC04185.1"
                     /translation="MKQPTYEYGVLPATGFVREKQLIGGGILPFSAKTLWRKVKDGTF
                     PAPVKLGERMTAWKAESVREWLDSRV"
     gene            complement(468..1139)
                     /locus_tag="PLY05_12640"
     CDS             complement(468..1139)
                     /locus_tag="PLY05_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04186.1"
                     /translation="MNTSEGLIPKWDKWLNMIDATDFQLVALSLNYEPPSTLKTITRF
                     QGLLCYAQRLSIIKNHITNKQLPYEQRRCSSTDKFENYVQLNAFVKWAVDVMAWDLPP
                     ELLTVYNAPAPAASSQKLIEENELLRQQLSDAQAKIDVLKKQLNEYQEKPIAIKTENK
                     VMPLIYHLCCQLLNNGKPVERPVQKTLSFITDDLKNKRGIELGSKALDDYYKLGEKII
                     NEKNR"
BASE COUNT          360 a          279 c          266 g          469 t
ORIGIN      
        1 gtaggtgtta atgtttaaaa ggctgtgatg tttgcgcgtc atggcctttg cttttatgtg
       61 tttttaactg ctcatacttg cctcgcaaat aatgaaaaac tgctaatgct aggtttaaag
      121 tcgtcattat ctgtactccc ttacacacga ctatctagcc actcacggac gctttcagct
      181 ttccatgccg tcatgcgttc gcccaatttt acgggggctg ggaacgtgcc atcttttact
      241 ttgcgccata aagtcttagc actaaagggc aaaatgccgc cgccaattag ctgtttttct
      301 cttacaaaac ctgttgctgg taatacgcca tactcataag ttggttgttt catgtttggt
      361 tgcctcttgt ctgggtatga cacaaggtag caatctaaaa aaatcctctt ggaatattcc
      421 aaaactattt ttgaatattc taaatagaat ctgtattacc aatgctttca gcgatttttt
      481 tcgttaataa ttttctcacc tagtttgtaa tagtcatcta aggctttact gccaagctct
      541 atgcctcttt tgttttttag gtcgtcagta ataaatgaaa gtgttttttg cactggacgt
      601 tccacaggct tgccattgtt tagaagctga cagcataagt ggtatatcaa aggcataact
      661 ttattttcag tttttatggc tatgggcttt tcctgatatt cattgagttg ttttttcagt
      721 acatcaattt tggcttgagc atcacttagc tgttgtctga gtaattcatt ttcttcaatc
      781 agcttttggc tagatgctgc tggtgcaggt gcgttataga cggtgagtaa ttcgggtggt
      841 aaatcccatg ccataacatc aaccgcccat ttaacaaaag cattcaattg cacatagttt
      901 tcaaatttat ccgtgctgct gcatcttctt tgctcataag gaagttgttt attcgttata
      961 tgatttttaa tgatggatag tcgctgggca taacacaata aaccttgaaa acgagtaatg
     1021 gttttaagcg tagatggagg ctcgtagttg agtgataacg caacaagttg aaagtctgtt
     1081 gcgtcaatca tgtttaacca tttgtcccat tttggaatta atccctcgct ggtattcata
     1141 aaaagccctt atataaattg cccctaaaaa tagaaggcca taccaaaaca gttaagggca
     1201 agctgttctt tcgcctcgtc aggctaggta tggccaatac gctaactaac cttagtaaat
     1261 tgaccctgaa tgacgttgta atgttgacca ttggcgcatc gttccaaaaa gtccgcccac
     1321 ttatctaacg cctctttacg ctcacgcaaa aattgatact ggttgtaatg cgcc
//
LOCUS       DAOQMB010000622         1366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_81106_length_1366_cov_4.630816, whole genome shotgun sequence.
ACCESSION   DAOQMB010000622 DAOQMB010000000
VERSION     DAOQMB010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81106_length_1366_cov_4.630816"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLY05_12645"
     CDS             <1..478
                     /locus_tag="PLY05_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04187.1"
                     /translation="MVLQEFNREHQLGYQFEELILIEPSALALKEAVKNNSLHYTHIK
                     QHCGKFDSLTPNNLLTQHQVTIHLLSNVLDLPNIDITALTNCIKQSLSGVNIFVCCSP
                     NYGQAVSGINQFCEAFDVANESTYYCKDSCEVESFEMRSQRVCTYSVPRYAQIFVI"
     gene            492..1259
                     /locus_tag="PLY05_12650"
     CDS             492..1259
                     /locus_tag="PLY05_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HNC04188.1"
                     /translation="MTLDLFAETHSLPLVVDGLKLPQLAGYDCQTIHFEHTQGYKITI
                     PQGELLYFPQFFTAKIADRALAYLLANSAGHSLPEELSATTPWLNIPWQQDEIKMYGK
                     QIPLPRLSSWHGDSDHPYTYSGITLQSLPWNAALDYLRDQLQQLTQRRFNSVLLNLYR
                     DGQDHISWHTDDEPELGKNPMIASVNFGESRRFLLRRHEKERQAHEKLEVLLSHGSVL
                     IMQGETQHYWQHSVPKQAKVLKPRINLTFRQIFPHTV"
BASE COUNT          397 a          296 c          264 g          409 t
ORIGIN      
        1 catggtgtta caagagttta atcgtgagca tcaattaggc tatcagtttg aagaactaat
       61 tttgattgag ccttctgctt tagccttaaa agaagcggtt aaaaataata gcttgcacta
      121 cacgcatatc aaacaacatt gtggcaaatt tgacagcctt acccctaata acttattaac
      181 ccaacatcaa gtcactattc atttattgtc taatgtctta gatttaccta acattgatat
      241 aactgcctta acaaattgca tcaaacaaag tttgtctggc gtcaatattt ttgtctgctg
      301 tagccctaat tatggccaag cggttagtgg cattaaccaa ttttgtgagg cgtttgatgt
      361 tgctaatgaa agtacctatt actgcaaaga ttcttgtgag gtcgaaagct ttgaaatgcg
      421 ttcgcaacgt gtttgtacat acagcgtacc acgctatgcc caaatatttg tgatttaata
      481 acgaggctat tatgacctta gatttatttg ccgagacaca ttcattgcca cttgtcgttg
      541 atggtctaaa actgcctcag ttagcaggtt atgattgtca aaccatacac tttgaacaca
      601 cacaaggcta caaaatcacc attcctcaag gcgagctgct ctactttcca cagttcttta
      661 cagccaaaat tgccgataga gctttggctt atttgttggc caacagcgca ggacacagcc
      721 tacccgaaga gttaagtgcc acaacacctt ggttgaatat tccgtggcaa caagacgaaa
      781 ttaaaatgta tggcaagcaa attcccttgc cgcgtttgtc ttcttggcat ggcgacagcg
      841 accaccccta cacctattca ggaattaccc tacaatcttt gccgtggaat gccgcgcttg
      901 attatttacg cgaccaactg caacaactta ctcaacgacg ctttaatagt gtgttgctta
      961 atttgtatcg agacggccaa gaccatattt cttggcacac cgatgacgaa cccgagctag
     1021 gcaaaaatcc tatgattgcc tccgttaatt ttggtgagtc acgacgtttt ttgttgcgtc
     1081 gtcacgaaaa agaacgtcaa gctcatgaaa agctagaagt tttattgagt catggctcgg
     1141 tgttgattat gcaaggtgaa acacaacact actggcaaca cagtgtgccc aaacaagcca
     1201 aagtgcttaa accacgcata aatttaacct ttagacaaat ttttccacat actgtttagg
     1261 ggcttgtata ggccaaacgg tacaagtgca acaaatattt aacatctgat gccgcacgat
     1321 gctgtggcgt catttgttgt ttggcttgtt gtttaaggtt ttttat
//
LOCUS       DAOQMB010000623         1365 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_81207_length_1365_cov_6.250382, whole genome shotgun sequence.
ACCESSION   DAOQMB010000623 DAOQMB010000000
VERSION     DAOQMB010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81207_length_1365_cov_6.250382"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1365
                     /locus_tag="PLY05_12655"
     CDS             <1..>1365
                     /locus_tag="PLY05_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04189.1"
                     /translation="KVKSIQALTDGDSLIFDALDAATFLMVEAVDKSPLSRTLATADL
                     NALFVGTNYNEGTLQAIASNDLKLIIDASTNTAVLNGELKLQRKKRNYSNGTWTWVND
                     GIAKGFTITNFKISYPSKTASTKEFTVAIDANSKIKTANVELGFASDSQNRVTAYFDA
                     ANTLQSHIDAASSTNTAHLPVKVEAKLDRITLKVLNAPASEMSQFVGNATLSGKRLNL
                     DTTTGGKRLWPMPDLATLSGKFSSADGDMVESTASVALSEMNPLVSPEQNFISTGFYN
                     YQYNPQANTITLKPKKGTIYYSNATQVVLSMDSQYCAGGQKRLMANVGYSWLGNCTTN
                     TDAAVAYKEFVTTYNYWWALRANVDSDGMYIPVYPANFDYKSTNAQVDGKLFYSEDTV
                     SEDATHQIKGTITANTKIKLVGNAAADIEALVTANYAGQQNGNLTMTVRAGEEKIDIT
                     GTTQ"
BASE COUNT          455 a          238 c          276 g          396 t
ORIGIN      
        1 agaaagttaa gtccattcaa gcattaactg acggtgatag tttgattttt gatgccttgg
       61 atgcagcaac ctttcttatg gtagaggcag ttgataaaag cccattatct cgcactttgg
      121 caaccgcaga tttaaacgca ttgtttgtcg gtacaaatta taatgagggt actttacaag
      181 caattgcatc taacgattta aaattgatta ttgatgcttc cacaaatact gctgtattaa
      241 atggtgaatt aaaattacag cgtaaaaagc gtaattatag taatggcaca tggacatggg
      301 tgaatgatgg catagcaaaa ggctttacca tcaccaattt caaaatcagc tatccttcaa
      361 aaacggcctc tacaaaagag tttactgtag ccattgatgc taacagtaaa attaaaaccg
      421 ctaatgtaga gttaggtttt gccagtgata gtcaaaatcg agtaacagcc tattttgatg
      481 ctgcaaatac cttacaaagt cacattgatg cagctagcag cacgaatact gcgcatttgc
      541 ccgtcaaagt agaagccaaa ttagatcgca tcaccttaaa agtattaaac gcacctgcga
      601 gcgaaatgag tcaatttgtg ggtaatgcaa cgctatcagg taaacgttta aatttagata
      661 ctacgacagg tggcaaacgt ttgtggccaa tgccggattt agcaacattg tcgggtaagt
      721 ttagcagtgc tgatggtgat atggttgagt caactgcaag tgttgcttta agtgaaatga
      781 atccacttgt gtcacctgag caaaacttta ttagcacagg cttttataat tatcaatata
      841 acccacaagc taataccatt accttaaaac ctaagaaagg cacaatttat tatagtaatg
      901 caacacaagt tgtattatca atggacagtc aatattgtgc gggcggacaa aaacgcttga
      961 tggctaatgt tggttattct tggttaggta attgcacaac aaatacagat gcggccgtag
     1021 cttataagga gtttgtgacc acttataatt attggtgggc attaagagca aacgttgata
     1081 gtgatggtat gtatatccct gtttatcctg ccaattttga ttataaatcc acaaatgctc
     1141 aagttgacgg taaattattc tattctgaag atactgtatc cgaagatgcc actcatcaaa
     1201 tcaaaggaac aattacggct aatacaaaga ttaaattagt cggtaatgca gccgctgata
     1261 ttgaggcttt agtgactgct aattatgcag gccaacaaaa tggtaattta accatgactg
     1321 tgcgtgcagg tgaggaaaaa attgatatta ctggtacaac tcaaa
//
LOCUS       DAOQMB010000624         1361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_81656_length_1361_cov_5.532159, whole genome shotgun sequence.
ACCESSION   DAOQMB010000624 DAOQMB010000000
VERSION     DAOQMB010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81656_length_1361_cov_5.532159"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1177
                     /locus_tag="PLY05_12660"
     CDS             2..1177
                     /locus_tag="PLY05_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005209830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNC04190.1"
                     /translation="MLNTSFSPWPSFTQEEADAVSRVLLSNKVNYWTGQECREFEKEF
                     AAWCGSQKAIALHNGTVAIDLALVASGVKAGDEVVVTPRTFLASASSIVSVGAIPVFA
                     DVDKDSQNITAETIAKVLTPKTKAIICVHLAGWPCEMTAINELAKLHGLIVIEDCAQA
                     HGASYKGVSVGSLADAAAWSFCQDKIMTTGGEGGMLTTNNLELWYKAWSYKDHGKDWD
                     AVYNRQHPAGFRWLHESFGTNWRMLEMQGVIGRIQLTRMADWHQARKQNATKILAACA
                     ESNALRVPQVPDYIEHAWYRAYAFVKPEKLATGWSRDRIMHEINALGVPCYSGSCSEV
                     YLEKAFDNTGWRPQERLPVAKELGETSLMFLVHPTLTEIEIEKTCSAIKSVMAQAQA"
BASE COUNT          410 a          217 c          320 g          414 t
ORIGIN      
        1 aatgcttaat acatcttttt caccatggcc gagttttact caagaagagg cggatgccgt
       61 tagtcgagtt ttattgtcaa ataaagttaa ctattggact ggtcaagaat gtcgtgaatt
      121 tgaaaaggag tttgcagcat ggtgtggtag ccaaaaagca attgccttgc ataatggcac
      181 ggttgctatt gatttagcgc ttgttgcttc aggtgttaag gcaggtgacg aggttgtggt
      241 gacgccacgt acgtttttag cttctgccag tagtattgtt tctgttggag ctatccctgt
      301 ttttgctgat gtagataaag attctcaaaa tattaccgct gaaacgattg ctaaagtatt
      361 aaccccaaaa actaaagcaa ttatttgtgt gcatttggct ggatggccat gcgaaatgac
      421 ggcaattaat gagcttgcaa agttgcatgg tttaatagta attgaagatt gtgcacaagc
      481 acatggtgct agctataaag gtgtttccgt tggtagttta gcagacgctg ctgcatggtc
      541 attctgtcaa gataaaatta tgacgacagg tggcgaaggt gggatgctaa caaccaataa
      601 tttggagtta tggtataaag cttggtctta taaagatcat ggcaaagatt gggatgctgt
      661 ttataatcgt caacaccccg caggctttcg ttggttgcat gagtcttttg gtactaattg
      721 gcgtatgtta gaaatgcagg gagttattgg gcgcattcag ttaacacgta tggcagactg
      781 gcatcaagca cgtaagcaaa atgcaacaaa aatcttagca gcatgtgccg agagtaacgc
      841 attaagagtg cctcaagttc ctgattatat tgaacatgcg tggtatagag cttatgcttt
      901 tgtcaagcct gaaaaattag caactggttg gtctagagat cgtattatgc atgaaattaa
      961 tgcattgggt gtaccatgtt atagcggttc atgctctgag gtatatctag aaaaagcatt
     1021 tgataataca ggttggcggc cacaagaaag actacctgtt gcaaaagaat tgggagaaac
     1081 aagtttaatg ttcttagttc atccaactct tacggaaata gaaatagaga aaacatgtag
     1141 tgcgattaaa tcagtaatgg cacaggctca agcataaata aataaggcaa actaataatt
     1201 tgccttattt attgtaggaa atttcatgaa aggtattttt gcacaaatat tagccttgcc
     1261 aaggcaaata aaacgcgtag ttatgatttt ggccgatgtt atagccgtgc caattttatt
     1321 atggtgcatg ttagcattac gttatgacac gttaaatcct c
//
LOCUS       DAOQMB010000625         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_81888_length_1359_cov_16.610429, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000625 DAOQMB010000000
VERSION     DAOQMB010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81888_length_1359_cov_16.610429"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..>1359
                     /locus_tag="PLY05_12665"
     CDS             479..>1359
                     /locus_tag="PLY05_12665"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HNC04191.1"
                     /translation="MSLTDTRCKGAKPKEKDYKISDEKGLYMIIKITGAKWWRFDYTF
                     DGKRKTLSLGTYPDISLKIARERRDEYRRMVATGIDPSANRKASKLVESIEVQDCFKL
                     IALEWLNVKKSEWVSSHYSKIESRLKREILPWLGDKAITEIKPIDILTILKRIQERGT
                     LETAHRAKSDIGQIFRYAIQTDRAKDNPTIHLSGALKTPKEKHMASIVEPEKVGDLLR
                     QIDGYQGTFIVRCALQLAPLVFVRPGELRQAEWNDINLETAEWRFKASKTHQDHIVPL
                     SKQALEVLKTIQPYSGHG"
BASE COUNT          456 a          286 c          230 g          387 t
ORIGIN      
        1 gctacttagc ggttttttaa tccacaatca aatccaaaga accttaatac catctaatcc
       61 atttttacgg taacctcaac ggtaactgca cttttcgcta ggttgttacc gtaatgtctt
      121 actccaaaga gctacaaaaa ctagattctt gctttgcaac ttccatgact ttaactcagt
      181 cttacgccct attttcattt agacgataca tcaaaaatca ttctctcaag tcaatttctt
      241 cacaggctct tattgctcac tttgcaaaag aattgttaac ttttgtaaat aaacatctaa
      301 tttatctctc aaattttcat ccaaccaaat ctaacaagct ccaaaaaaca ccaacaaaac
      361 acaaaactat attacaagcc attgaattta ctaaatttta ttaaaagtct atatttcaaa
      421 tcagaactaa gctaataaat tcttaaatcc tcaaacactg ttctattgga gcaaaaaaat
      481 gtcattaacc gatacccgtt gtaagggagc gaaacctaaa gaaaaagatt acaaaatctc
      541 tgacgaaaaa gggctctata tgattattaa aatcactgga gctaaatggt ggcgttttga
      601 ttataccttt gacggtaagc gcaaaacgct atctttaggg acataccccg acatcagcct
      661 aaaaattgct agagagcgac gtgacgaata tcgacggatg gtagcgacag gtattgaccc
      721 ctccgccaat cgcaaagcca gtaaacttgt tgaatcaatt gaggtacaag actgcttcaa
      781 attaattgct cttgaatggc ttaatgttaa aaagtctgaa tgggtttcta gccattattc
      841 aaaaattgaa tcccgcctca aacgagaaat cttgccttgg ctcggtgata aagccattac
      901 tgaaattaaa ccgattgata ttttaaccat tttaaagcgt attcaagaaa gaggcactct
      961 tgaaaccgcg catcgcgcaa agtctgatat tggtcaaatt ttccgttatg cgattcaaac
     1021 agatagagct aaagacaatc cgacgattca cttgtctggt gcgctcaaaa cgccaaaaga
     1081 aaagcacatg gcatctatcg ttgagcctga gaaagttggc gatttactgc gccaaattga
     1141 cggttatcaa ggcacattta tcgtacgttg tgctttgcag cttgcccctt tagtgtttgt
     1201 gagacctggt gaactaagac aagcagaatg gaatgatatt aacttggaaa ctgccgagtg
     1261 gcgttttaaa gccagcaaaa cccatcaaga tcatatcgtg ccattatcaa aacaagcctt
     1321 agaagttttg aagacgattc agccctattc aggtcatgg
//
LOCUS       DAOQMB010000626         1359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_81890_length_1359_cov_14.990031, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000626 DAOQMB010000000
VERSION     DAOQMB010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81890_length_1359_cov_14.990031"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1201
                     /locus_tag="PLY05_12670"
     CDS             140..1201
                     /locus_tag="PLY05_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HNC04192.1"
                     /translation="MKIAIPRERKTLEGRVALTPDAVGLLIKAGHQVILENDAGLNSG
                     YDNQQYQAIGATIVQNLADLYQADLIVKVKEPMPEELALLKPQQLLFCYLHLAAYPHV
                     LTALLQQKVTALAFETLTVNQRLPLLAPMSAVAGRIAVQLAIQYLQKNHQGAGVLLGG
                     HFNIYHRGKVLVLGAGVAGSQAALMASALGAEVFVFDKSLEALDALYQKDSNLQVQLF
                     DTHKISQLLASIDVVIGAVLIKGANAPKVLSIAQQQLLAKGRVIVDIAIDQGGCIEGI
                     EATDWQQPAYQKNNLTYIAVSNMPAAVPRTATQLLSQAITPYVLALANGKLATDTVLQ
                     TAIALQDGKIIHSALQNFA"
BASE COUNT          435 a          265 c          265 g          394 t
ORIGIN      
        1 taattatgcc taaaattaaa gaaacgcctt ggcttatagc ttatgcaatt attacttttt
       61 gtctatattt aagacctcac cccagccctc tcctaaaagg agagggagct acatagttag
      121 tatttacaag gagtccaaaa tgaaaatagc tattccacgt gaacgtaaaa ccttagaggg
      181 gcgagttgct ttaacacctg atgccgttgg tctattaatc aaagcaggac atcaggttat
      241 tttggaaaat gatgcaggat tgaatagcgg ttatgacaat cagcaatatc aagcaatcgg
      301 tgcaacgatt gtgcaaaact tagccgactt gtatcaagct gatttgatcg ttaaagtaaa
      361 agaacccatg cccgaagagt tagccctatt aaaaccacaa caattattat tttgttattt
      421 acatcttgcc gcatacccac atgtattgac cgctttatta caacaaaaag taaccgcact
      481 tgcttttgaa accttaaccg ttaatcagcg tttgccctta ttagccccaa tgagtgccgt
      541 tgcaggccga attgcggtgc aattagccat tcagtattta caaaaaaatc atcaaggggc
      601 aggcgtttta ttaggcggac attttaatat ttatcatcgt ggcaaagttt tagttttggg
      661 tgcaggtgtg gcaggcagtc aagcggcatt aatggccagt gctttgggag cggaagtatt
      721 tgtgtttgat aaatctttag aagcattgga cgcgctttat caaaaagact caaatctaca
      781 agtacaactt tttgatacac ataaaattag ccaattatta gcgagtatag atgtagtgat
      841 tggtgcagtg ttaattaaag gcgcaaatgc tcctaaagta ttatctatag cccaacaaca
      901 gttattagct aaagggcgag tcatcgtgga tattgctatc gaccaaggtg gttgtataga
      961 aggtatagaa gctaccgatt ggcaacaacc tgcgtatcaa aaaaacaatc tcacttatat
     1021 tgcggtgagt aatatgcctg cggctgtacc acgcaccgcc acccagttat tatcacaagc
     1081 gattacgcct tatgtacttg ctttagccaa cggtaaattg gccacagata ccgttttaca
     1141 aacagcaatt gcattacaag acggcaaaat tattcattca gccttacaaa actttgcata
     1201 aaaggcaaat gttgacgtga caaaatggat tatgtgcgcg aaatggatgt ttttttaaga
     1261 aaatcgcata tactccacaa gctttaaatt acaaactttg aaaactcacg gattttagtt
     1321 gtgaccgctt ctttacccaa taatcctgca ttaaatacc
//
LOCUS       DAOQMB010000627         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82015_length_1358_cov_8.320031, whole genome shotgun sequence.
ACCESSION   DAOQMB010000627 DAOQMB010000000
VERSION     DAOQMB010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82015_length_1358_cov_8.320031"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..398)
                     /gene="cas2"
                     /locus_tag="PLY05_12675"
     CDS             complement(90..398)
                     /gene="cas2"
                     /locus_tag="PLY05_12675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNC04193.1"
                     /translation="MNKHTPLLLCYDIRDPKRLQRVHKCVKQVGMPLQYSVFYLEMSN
                     ADVTQLLNRLSKIIDGSRDDVRLYAISRFEDIALMGASLLADGVQVFTHGQPILKKSS
                     "
     gene            complement(395..931)
                     /locus_tag="PLY05_12680"
     CDS             complement(395..931)
                     /locus_tag="PLY05_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04194.1"
                     /translation="MKIIFEYLFSQEEDCLEGNSKRTMYKHTPTNGSYVADKDYFTQQ
                     HYGVGFTVYADAFCDQTMLEMINVALNSIYLSLDNHTHNPNVIKQLWNIVNQTTNDSF
                     YDDCKNYVININTQQSVFLVSQRFDTPLSVKIDAQCTRLFLWAYLTMLESPSTKIKLE
                     IELPIEGFNILRTNGEST"
     gene            complement(968..1246)
                     /locus_tag="PLY05_12685"
     CDS             complement(968..1246)
                     /locus_tag="PLY05_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04195.1"
                     /translation="MLTVVLGEQLMAYGLAAGTSLRNRLFNDLHDLMEYWIQGDILQW
                     LQRFAEVNDTLKRDFYSHQYNALSEKITLILSQLQEMLEQYALEQLHG"
BASE COUNT          444 a          252 c          224 g          438 t
ORIGIN      
        1 ctgaaacgac cgtattaagc tatcataaaa aatttcacaa tagtagcctg ttttgcataa
       61 tctattaaaa aaatttggca gcttaagttt tatgacgatt tttttagaat cggctgacca
      121 tgggtaaaaa cttgcacacc atcggccagt aaagatgcac ccattaaggc aatatcctca
      181 aaacgactaa ttgcatataa tctaacatca tcccttgagc catcaataat tttagataaa
      241 cgatttaata attgagtgac atcagcatta ctcatttcta aataaaaaac cgaatattgt
      301 aatggcatac ctacctgctt aacgcactta tgaacacgtt gtaagcgttt ggggtcacga
      361 atgtcataac ataataataa gggagtatgc ttgttcatgt tgattctcca ttagttctca
      421 aaatattaaa accctctatt ggtaattcaa tctctagttt aatttttgta ctaggtgatt
      481 ctagcatggt tagatatgcc cacaaaaaaa gtcgtgtaca ttgcgcatct atctttaccg
      541 aaagcggtgt gtcaaaacgt tggctaacta aaaatacgct ttgttgtgtg tttatgttaa
      601 taacataatt tttacagtcg tcataaaaac tatcattggt tgtttgatta acaatattcc
      661 atagctgttt aataacattg gggttatgag tgtgattatc taaagataaa taaatgctat
      721 ttagggcaac attaatcatc tcaagcattg tttgatcaca aaacgcatca gcataaacgg
      781 taaaacccac accataatgt tgttgagtaa aataatcttt atcggccaca taactaccat
      841 ttgtaggagt atgtttatac atggtgcgtt tggagttgcc ttctaaacaa tcctcttctt
      901 gagaaaataa gtattcaaaa ataatcttca tgatcgctgc tctaattaaa tgtggtgatg
      961 agaaaaatta accatgtaat tgttctaaag catactgttc taacatctct tgcagttggc
     1021 tgagtatcag cgttattttc tcagaaagtg cattatattg atggctataa aaatcgcgtt
     1081 ttaacgtgtc attaacttcg gcaaaacgtt gtagccactg caaaatatcg ccttgtatcc
     1141 agtattccat taaatcatgt aaatcgttaa atagtctgtt tcgcaagctt gtgccagccg
     1201 ctaaaccata agccattaat tgttcaccca atacaaccgt taacatacgt ttaccttgtt
     1261 gatataactg ggtaaaaaca ctgtctagct gacgatgttt aagttgacat cctccccgcc
     1321 ctcaatactt cgactaagct cagtaaccga gggcgagg
//
LOCUS       DAOQMB010000628         1358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82020_length_1358_cov_4.795088, whole genome shotgun sequence.
ACCESSION   DAOQMB010000628 DAOQMB010000000
VERSION     DAOQMB010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82020_length_1358_cov_4.795088"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLY05_12690"
     CDS             complement(<1..655)
                     /locus_tag="PLY05_12690"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNC04196.1"
                     /translation="MFASLMACGGGGGDSMATTNNAPTPDPKPTTPVLAAEEAAAIKD
                     DPAVYDVEKNQVLTATVTTTDTSTWTFADVNNDTDDSDAFVPEVDAHIIIDGFADDAK
                     SKNATLRLRGHSSRLSEQKSYRIKLAKDAGLWRGEQTLQFNKHPYDLTRVRNKLAFDL
                     FRDIPHIPSLRTQFVKMNITNFDKTGLQYGSKDYGLFTHVEKMGKEYLTNRGLPADGN
                     "
     gene            885..>1358
                     /locus_tag="PLY05_12695"
     CDS             885..>1358
                     /locus_tag="PLY05_12695"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HNC04197.1"
                     /translation="MSITLNQYLVQAKIPAQLQQLLTQMSVACKAISVAIGKGALAGV
                     LGQAGSDNIQGEEQKKLDVISNDIVLQAALGSGSIAGLASEEMDDIFPLPDGTPLGDY
                     LMLFDPLDGSSNIDVNVSVGTIFSIIKKPLEAESVVVTEDFLQAGNQQVCAGYAVY"
BASE COUNT          381 a          302 c          272 g          403 t
ORIGIN      
        1 tattaccatc agcaggcagg ccacgattag tcaaatactc tttacccatt ttttcgacat
       61 gggtaaacaa accatagtct ttgctgccgt actgtaaccc cgttttgtca aaattggtga
      121 tattcatttt cacaaactgg gtacgcaaac taggaatgtg tggaatatct ctaaacaaat
      181 caaaagctaa tttattacgc actcgggtta aatcgtaagg atgcttatta aattgtaaag
      241 tttgctcacc acgccataaa cctgcatctt tagctaattt aattcgatac gatttttgct
      301 ccgataaacg cgaactatgg ccacgcaaac gtaaagtcgc atttttgctt tttgcatcgt
      361 cagcaaaacc atcaataata atatgcgcat caacctctgg cacaaaagca tcactgtcat
      421 cagtatcatt gttaacatcg gcaaaagtcc atgtactggt atcagttgtc gttacagtag
      481 ccgttaacac ttgatttttt tcgacatcat ataccgcagg gtcatcttta atggccgctg
      541 cttcttctgc ggccaagacg ggggttgttg gcttggggtc aggcgtaggc gcattattag
      601 tagtagccat actatcgccc cccccaccgc cacaagccat taaagatgca aacatgattg
      661 ccaaaactaa tggcttgagt gttgtaattg ttgacattga ctagccctgt aacccataaa
      721 aaattttgcc aaatctatca cattttgtct gtcctgccaa gttaataacg ataactggca
      781 tctgaaggca atctcgggga taattaaccc tctttgaact tgcgcgatgc ttgcggcaat
      841 ctgcgtaagt ggtctcttta tgtctgctat gccttggagc cttcatgtct attaccctta
      901 atcaatattt ggtacaagcc aaaatccctg cgcaattaca acaattactc acccaaatga
      961 gtgtcgcttg caaagccatt agcgtggcca ttggcaaagg cgcacttgca ggcgttttag
     1021 gtcaagctgg ctccgacaat attcagggtg aagaacaaaa aaaattagac gtgatttcta
     1081 atgacattgt gttacaagcc gcactcggtt caggcagtat tgcaggttta gcatccgaag
     1141 aaatggacga tatttttcca ttacctgacg gcacacctct aggtgattat ttaatgttat
     1201 ttgacccttt agatggctct tctaatattg atgtaaatgt gtcggttggt acgatttttt
     1261 cgattatcaa aaagccgctt gaagccgaat cagtggtcgt cacagaagac tttttgcaag
     1321 caggcaatca gcaggtatgt gcaggctacg ccgtctat
//
LOCUS       DAOQMB010000629         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82238_length_1356_cov_6.356649, whole genome shotgun sequence.
ACCESSION   DAOQMB010000629 DAOQMB010000000
VERSION     DAOQMB010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82238_length_1356_cov_6.356649"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..1016)
                     /gene="suhB"
                     /locus_tag="PLY05_12700"
     CDS             complement(195..1016)
                     /gene="suhB"
                     /locus_tag="PLY05_12700"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HNC04198.1"
                     /translation="MQPMLTMAVRAAKQAGELIAKAAQQLQFVDVEVEAKGINDFVTR
                     VDRDAERLIIENLRKAYPTHSFQGEESGLIEGQGVGQDWLWIIDPLDGTTNFIHGFPQ
                     YAVSIAVQHKGQTEHAVVYNPITEECFSASRGHGTTLNGRRLRVTQRKNLDGALIGTG
                     FPFRPDQHSYLDSYLSAFKAVALQTAGLRRAGAASLDLAFVAAGRLDGFFELGLAPWD
                     MAAGDLLIREAGGMVCDTAGGHDYLKSGNIVAGNIKIMKALVTTIAPHLPEYMRR"
     gene            1248..>1356
                     /locus_tag="PLY05_12705"
     CDS             1248..>1356
                     /locus_tag="PLY05_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HNC04199.1"
                     /translation="MFSHIRIVLVNTSLPANIGSAARALKTMGLRQLVLV"
BASE COUNT          429 a          286 c          262 g          379 t
ORIGIN      
        1 cctgtttgca acgcaataac agtaataagc ctatgatgat aaaagcaaaa gcatacaaat
       61 attcagtatg ccaaatgtta tgccatgcag aaaaagagat tggcatgtta aatccctact
      121 attttttaat aattaaagca ttcttattaa aaaacaaaaa gccccaaaaa tggggctttt
      181 tagcaaaact cacattaacg gcgcatatac tcaggtaaat gcggtgcaat ggtggttacc
      241 aaggctttca taattttaat attaccagcg acgatgttgc ctgattttaa gtaatcatgg
      301 ccacccgcag tatcacacac cataccacct gcttcacgaa tcaataagtc accagccgcc
      361 atatcccaag gtgctaaacc taattcaaaa aagccatcca aacgacctgc ggcaacaaac
      421 gctaaatcca aagaagctgc ccctgcgcgg cgtaaacctg cggtttgtaa agcaactgct
      481 ttaaaggcac ttaaataaga gtctaaatag ctatgttgat cgggacggaa tggaaagcct
      541 gtgccaatta atgcgccatc taagtttttg cgttgagtca cacgtaaacg acggccattt
      601 aaggttgtac cgtgaccacg actggcacta aaacattctt cagtaatcgg attataaaca
      661 acggcatgtt cggtttggcc tttatgttgt acggcaatac ttacggcata ttgcggaaaa
      721 ccgtgaataa agtttgttgt gccgtctaaa ggatcaataa tccataacca atcttgacca
      781 accccttggc cttcgattaa acctgattct tcaccttgaa aactatgtgt tggataagcc
      841 ttgcgtaaat tttcaataat taagcgttcg gcatcacggt caacacgagt tacaaaatcg
      901 ttaatgcctt tggcttcaac ttcaacatca acaaattgta attgttgggc agctttggca
      961 atcagttcgc ctgcttgttt agcggcacgc acggccatgg taagcatcgg ttgcatggat
     1021 gtaaactctt aaagaacagt aacaggattt acgcatttca ttgagattag cgcgttttgt
     1081 aaacataaaa ccgttgataa cggttgaaat gtgaacaaat aagcgataac cgcgtaagtc
     1141 ctaagtaaat aggataataa tccacagtag agccgcaaat tatagcagag acttggggct
     1201 gaatttgcta ccattagcca tctttttttc attattgatt ggataacatg tttagtcata
     1261 ttcgtattgt cttagtaaat accagtttgc ctgccaatat tggctctgcc gcacgtgcct
     1321 taaagaccat gggtttacgt caattagtct tagttg
//
LOCUS       DAOQMB010000630         1356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82258_length_1356_cov_4.285934, whole genome shotgun sequence.
ACCESSION   DAOQMB010000630 DAOQMB010000000
VERSION     DAOQMB010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82258_length_1356_cov_4.285934"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLY05_12710"
     CDS             complement(<1..188)
                     /locus_tag="PLY05_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04200.1"
                     /translation="MLHKIVTATMCLVSSVAIAGSIELSVAESEAYQGYTRLQHQHLQ
                     LIERVKDVEVTTNFDRKG"
     gene            complement(207..>1356)
                     /locus_tag="PLY05_12715"
     CDS             complement(207..>1356)
                     /locus_tag="PLY05_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04201.1"
                     /translation="LQQLAFMGLLPLAGYTMPVLNAHVPLITEPALANIKSWLKSMED
                     KKTGLLYYMDKGTAHFLSPLTTAKTAIWANCVDDKILLKKYAQAIVFAQQIMAKTYPE
                     LKGALASSYVRHKNGLVAGQYVYACEQLVCISALLKAYSHFKEDSFLIAAHDNAMFIK
                     RTFLNGYALGAWNQPFPIPFNYLTTNQAFENTMRNGVEYLWISAFLELYKHTHEYIWL
                     QLYQDALAFYKNSQADNGLWFDTFFPNKKTWAWYQTTNVVADNMLRCAIASTLVGEDK
                     QVDHFLQQLERHHDKGLIMGYLSTIDANNGFISQDKPYYDVVATGLLRNLYMLRGNYG
                     LASQCQQTLKLLSDSQSGYFWGRYQHDFSPVGKEKAVITTLWASANLF"
BASE COUNT          430 a          275 c          224 g          427 t
ORIGIN      
        1 tagcctttac gatcaaaatt tgtggtgact tcaacatctt taactcgctc tattaactgc
       61 aaatgctgat gttgcaaacg cgtataacct tgataagcct cagactctgc cacgctcaat
      121 tcaatcgaac cagctattgc gacagaagat accaaacaca tagtcgccgt tactattttg
      181 tgcaacatgc acttctcctt acttaactaa aaaagattgg ccgaagccca caaagttgtt
      241 ataacagctt tttctttacc tacaggtgaa aaatcatgct gataacgtcc ccaaaaataa
      301 cctgattggc tatcactcaa taactttagt gtttgttgac attgtgacgc taatccataa
      361 ttaccacgta acatatatag gttacgcaat aaccctgtgg caacaacatc ataataaggt
      421 ttgtcttggc taataaaacc attattagca tctatcgttg acaaataacc cataattagt
      481 cctttatcat gatgccgttc taattgttgt aaaaaatgat ctacttgttt atcttcacca
      541 accaatgttg aagcaatagc acaacgcaac atattatctg caacaacatt ggtagtttga
      601 taccatgccc atgtcttctt gtttggaaaa aatgtatcaa accataggcc attatcggct
      661 tggctatttt tataaaaagc taaggcatcc tgatagagct gtagccaaat atattcgtgt
      721 gtatgtttat ataattccaa aaatgcggaa atccataaat actcaacacc attacgcatg
      781 gtattttcaa aggcctgatt ggttgttaag taattaaagg gaataggaaa gggttgattc
      841 catgctccta aggcataacc attcaaaaac gttcttttga taaacattgc attatcatgt
      901 gccgcgatta aaaaagaatc ttctttaaaa tgactatagg cttttaaaag tgccgaaatg
      961 cacacgagtt gttcacaagc atacacatat tgtcctgcca ctaatccatt tttgtgacga
     1021 acataactac ttgctaatgc tccttttaat tcaggatagg tttttgccat gatttgttgt
     1081 gcaaatacaa tagcctgtgc atattttttt agcaatattt tatcatctac acagtttgcc
     1141 caaatagctg tttttgctgt ggttaagggt gataaaaaat gcgctgtgcc tttatccata
     1201 taataaagca accccgtttt tttatcctcc atagatttca accagctttt tatgttggca
     1261 agagctggct cagtgattaa aggtacatgt gcattgagga caggcattgt atatccagcc
     1321 aatggcaata gccccataaa agcgagctgc tgcaaa
//
LOCUS       DAOQMB010000631         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82363_length_1355_cov_10.083846, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000631 DAOQMB010000000
VERSION     DAOQMB010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82363_length_1355_cov_10.083846"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..1292)
                     /locus_tag="PLY05_12720"
     CDS             complement(87..1292)
                     /locus_tag="PLY05_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005054087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HNC04202.1"
                     /translation="MTTFNFEDALKQLQSGKKLTGTDGILTPLIKQLTEAALQAELEQ
                     HLQQQDELASNRKNGYTSKTIKTSSGSFELDTPRDRAGTFEPQLVKKHQTKLTDEIDS
                     KILSLFALGMSYKDIQQHISELYALSISDGMISVVTDRLIPELKQWQQRPLEVVYPFI
                     WLDAIHYKVKDNGHYVSKAVYTILGVNCEGQKELLGLYVSENEGANYWLTVLTDLHNR
                     GVKDILIACIDGLRGFPEAIAAIYPNTEIQHCIIHQIRNSLRYVGSKHHKAFMADLKP
                     VYRAVNLIAAESALDALETKWGQLYPVVIKSWRSNWQTLSAYFKYPDYIRKPIYTTNA
                     VEAVHRQFRKLTKTKGGFSSEQALLKLLYAGMMKASERWTMPIANWGQTMAQLSIHFQ
                     GRLEGIIKI"
BASE COUNT          364 a          300 c          268 g          423 t
ORIGIN      
        1 ataaaaagcc aaataatcaa tcacactcag tttcgcctct gcaactgttc ggaaagaggg
       61 tgttcaaatt tctgtgtcag cgccgtttag atttttataa tgccctctaa acggccttga
      121 aagtgaatag ataattgcgc catggtttgc ccccaattag cgataggcat agtccatcgc
      181 tcagaggctt tcatcatacc tgcgtagagt aatttgagta aagcctgctc actactaaaa
      241 ccgcccttcg ttttggtcag ttttctaaat tgacgatgaa ctgcctcaac agcattggtc
      301 gtataaattg gcttacgaat gtaatcaggg tacttaaaat acgccgacaa ggtttgccaa
      361 ttgctacgcc atgacttaat cactacaggg tacaattgcc cccatttagt ctctaacgca
      421 tccaaagccg attcagctgc gatgagatta accgcccgat aaacaggctt taaatccgcc
      481 ataaacgctt tgtgatgctt ggaccctaca taacgcaagc tattgcgtat ttgatggata
      541 atacaatgct gtatttcggt gttagggtaa atagcagcga tggcttcggg aaagcctctt
      601 aaaccatcaa tacaggcaat cagtatatct ttgacccccc gattgtgtaa gtctgtcagt
      661 acagtaagcc agtaatttgc gccttcattt tcagagacat ataaacccag tagctctttt
      721 tgcccttcac aatttacgcc taaaatcgtg taaaccgctt ttgatacata atggccgtta
      781 tctttgactt tgtagtggat agcatcaagc caaataaacg gatacaccac ttctaagggg
      841 cgttgctgcc attgttttaa ctcagggatt aaacggtcag ttaccacact aatcatgcca
      901 tcagagatgc ttaaggcata caattcgctg atatgctgtt gaatgtcttt gtagctcatg
      961 cctagcgcaa acagtgataa aatcttactg tcaatttcat cggtgagttt cgtttgatgc
     1021 tttttaacga gctgaggctc aaacgttcct gcacggtctc gaggggtgtc taattcaaaa
     1081 ctacctgaac tcgtttttat cgttttgctg gtgtagccgt ttttgcgatt tgaggccagt
     1141 tcatcttgtt gttgtaaatg ctgctctagc tccgcctgca aagccgcttc tgtgagttgt
     1201 ttgattaacg gcgttaaaat accatctgtg cctgtgagct tctttccaga ttgtaattgt
     1261 tttaacgcgt cttcaaagtt aaacgtcgtc atgtgtcatt tcctcttaat tcttacttaa
     1321 gtttaaaaaa ttgacacaga tttatgaata ctccc
//
LOCUS       DAOQMB010000632         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82391_length_1355_cov_3.858462, whole genome shotgun sequence.
ACCESSION   DAOQMB010000632 DAOQMB010000000
VERSION     DAOQMB010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82391_length_1355_cov_3.858462"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /locus_tag="PLY05_12725"
     CDS             complement(<1..915)
                     /locus_tag="PLY05_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04203.1"
                     /translation="MDDVLAPSTTLTSPHIIAQQPHVLTVLARYGATQNSFSFAKAWS
                     KDGTGALANVKKHTHTTHSIPTLDALYNALSELAQNHNACVIRGALTPIGEAFYSKHG
                     FVYRRNLEKLENNRKIAPHFHDVARAWACFDADDILTPHDMRTEAQQAVAWYVKNCLP
                     SCFKDVGYVWQLSGSAGHPTKDPKMLRAHVWVLLTAPRTSKELKAWAKNGITEGMTDL
                     SLFQPVQAHYTSNPVLTYCEYTDPCEQRLGIVHGAPLDVPIVSTVDLSHIKKTKAHKE
                     LCPKTQQAINAANVAGAFASTFQQTIALN"
     gene            complement(960..1256)
                     /locus_tag="PLY05_12730"
     CDS             complement(960..1256)
                     /locus_tag="PLY05_12730"
                     /inference="COORDINATES: protein motif:HMM:NF025456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04204.1"
                     /translation="MTIPHRVAILRHILKEHQSVSTQSQQQRLVLALTYLGSITTIEA
                     RDYLDIMHPSGRIKELKEQGWLIVSSRVRHIAPCGASHFIANYVLQTLPLARAA"
BASE COUNT          350 a          304 c          304 g          397 t
ORIGIN      
        1 gtttaaagca atagtctgtt gaaacgtact agcaaacgca cccgccacgt tagctgcgtt
       61 aattgcctgt tgtgtcttgg gacaaagttc cttgtgcgcc tttgtctttt tgatgtgcga
      121 taaatcaacg gtagagacaa tcggaacatc tagcggcgcg ccgtgtacga tgcctaaacg
      181 ctgttcgcat gggtcggtat attcgcaata agttaaaaca ggattacttg tgtaatgcgc
      241 ttgcactggt tgaaacaaac ttaaatcggt catgccctct gtgatgccgt ttttagccca
      301 tgcttttaat tctttagatg tacgcggcgc ggtcaagagt acccacacat gggcgcgtaa
      361 cattttaggg tctttggtcg ggtgtcctgc actccctgat aattgccaaa cgtaaccaac
      421 atctttaaaa caagagggta aacagttttt aacataccaa gcaacggcct gttgcgcctc
      481 tgtcctcatg tcgtgcggcg ttaaaatatc atcagcatca aaacacgccc acgccctcgc
      541 aacatcgtga aaatgagggg caatctttct attattttct agcttttcta aattacggcg
      601 ataaacaaaa ccatgcttac tataaaaagc ctcgccaata ggcgttaaag cccctctaat
      661 tacgcaagca ttatgattct gtgctaactc actaagcgcg ttataaagcg cgtctaaggt
      721 cggtatagag tgggttgtgt gtgtgtgttt cttgacgtta gctaatgcgc ctgtcccgtc
      781 tttgctccac gctttagcaa atgaaaagct attttgtgtc gcaccatatc gcgctaaaac
      841 ggttaaaacg tgcggttgtt gtgctataat gtgagggctt gttaaggttg tactgggggc
      901 taaaacatcg tccaatgtta gcccctcgca ccttttaagg ttatctggta tcagggtcat
      961 tatgctgccc tcgctagtgg taaagtttgt aaaacataat tagcaataaa gtgactagct
     1021 ccacacgggg caatgtgtcg cactctcgaa gatacaatca accaaccttg ttcttttagt
     1081 tctttaatgc gtccagacgg gtgcatgatg tctaagtaat cgcgcgcctc tatcgtggta
     1141 atgctgccca agtaagttaa agctaaaaca agtctttgct gttggctttg tgtcgatacg
     1201 ctttgatgtt cttttaggat atgcctaaga atggctacgc gatggggaat agtcattacg
     1261 cggcctcctt gcttacgtct aagcgcgatt ctatccatgc ctcaacatca gcacgaacaa
     1321 aagcaatgcg attgccaagt aatcgaattg tttta
//
LOCUS       DAOQMB010000633         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82442_length_1355_cov_2.453077, whole genome shotgun sequence.
ACCESSION   DAOQMB010000633 DAOQMB010000000
VERSION     DAOQMB010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82442_length_1355_cov_2.453077"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLY05_12735"
     CDS             <1..586
                     /locus_tag="PLY05_12735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04205.1"
                     /translation="GYEIAKQMCYAKENQLTTCQINIVPKASVSVGQLLSVLRWDVNP
                     EDLDSHLFKYDASGNRVYQIYYANQVSGADSLDVDDRDSYGPETVTIDKLDPKANYVY
                     AIHHFAGSGSITTTSQAQVSVLQNGKPVRIYKAPLAGFGQWWKVFEVKNGQVIPCQQD
                     CMFSIAPQPTARSRALPTPRWLLDAMSAPMPPKK"
     gene            652..>1355
                     /locus_tag="PLY05_12740"
     CDS             652..>1355
                     /locus_tag="PLY05_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HNC04206.1"
                     /translation="MYRVFLYAWLLGMVGMTYATPQAKIINGQTVTDNEAPWQVALIS
                     NNQQPYTSYFCSASIIGDKWLVTAAHCAERSNSKPFYGVIGTTDLRNTQKAQIILIKQ
                     RLVHPKYNSSNFDNDIALLELAQTIDFVACGQDCKAINWLKAQHEPLYAVVNTKVWVH
                     GWGVIKDCKQDIETCTTEEKAFEALYPSKLQKAELKIDNCLAQGSLHSPQQITSSMMC
                     ANAIDEQKPSDACEGD"
BASE COUNT          434 a          259 c          279 g          383 t
ORIGIN      
        1 cggttatgaa atagcaaagc aaatgtgcta tgcaaaagaa aaccaattaa caacttgcca
       61 aattaatatt gtgccaaaag ccagcgtgag tgtaggacag ctattgagtg ttttacgttg
      121 ggatgttaat cctgaagatt tagactccca tttgtttaaa tatgatgcta gcggcaatcg
      181 tgtgtatcag atttattatg ctaatcaagt aagtggtgca gatagccttg atgtggacga
      241 tagggatagt tatgggccag aaaccgtgac tatcgataaa ttagacccta aagctaatta
      301 tgtctatgct atacatcatt ttgctggtag tggctcaatt accactactt cgcaagcgca
      361 agtaagcgta ttgcaaaatg gtaaacctgt acgcatttac aaagcaccat tagcaggttt
      421 tgggcagtgg tggaaagtct ttgaagtcaa aaatggccaa gtcattcctt gccagcaaga
      481 ttgtatgttc tctattgcac ctcaacctac ggcgcgtagt agagcgttac ccacacctcg
      541 ttggttatta gacgcaatga gtgcgcctat gccacctaaa aagtaaatcg gctaaaaata
      601 acccgcataa ctgcgggtta ttttttagtg cattacctat ttgagaaaat aatgtataga
      661 gtttttttgt atgcttggct attaggtatg gttggtatga cctatgccac cccacaagcc
      721 aaaatcatca atggacaaac ggtgacagat aacgaagcac cgtggcaagt cgcgcttatt
      781 tccaataatc aacaacctta cacctcttat ttttgctctg ccagtattat aggtgataaa
      841 tggctagtaa cagccgcgca ttgtgccgag cgtagcaata gcaaaccttt ttatggcgtt
      901 attggcacaa ccgacttacg caatacgcaa aaagcgcaaa tcattttaat taaacaacgc
      961 cttgtgcatc ctaaatacaa ttcatccaat tttgataacg acatcgcctt attagaatta
     1021 gcacaaacca ttgattttgt cgcttgtggc caagactgta aagccattaa ctggctaaaa
     1081 gcacaacatg agccacttta tgcggtggta aatacaaaag tatgggtgca tggttggggc
     1141 gttattaaag attgtaaaca agatattgaa acctgtacta ccgaagaaaa agcttttgag
     1201 gctctatatc cctctaaatt acaaaaagca gaattaaaaa ttgataattg tttggcacaa
     1261 ggttcattac acagtcctca acaaattaca tcaagtatga tgtgtgctaa tgcgattgat
     1321 gagcaaaaac caagtgatgc ttgtgagggt gatag
//
LOCUS       DAOQMB010000634         1352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82707_length_1352_cov_12.400925, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000634 DAOQMB010000000
VERSION     DAOQMB010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82707_length_1352_cov_12.400925"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(241..>1352)
                     /locus_tag="PLY05_12745"
     CDS             complement(241..>1352)
                     /locus_tag="PLY05_12745"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNC04207.1"
                     /translation="ESNFVFNYGLNAETETAVSLVMPVRQQSYCSGELMGIFSMNRPE
                     GYLRYMVEERLKRLGAPSDMFLLYLAGAHQIGRLNYALDGQIAPKEKGETLQTLLQSS
                     STDLFIYLVDKYALTSGISGVQPKTLVPLAQALPAGHSSLALDTVIVKAEGQDYVGIA
                     RNEYLCLSVAKQAGFSVPDFWLSDDGLLLVMSRFDRKADGSALGFEDMAVLTGRNTAR
                     KYEGSYEMIAKAVAIFTSEYSHIELQRLFERVALSCKLGDGDAHLKNIGLLYEQPSAR
                     RQLAPIYDVVCTNIYPELDGQLALKLNKKKSFPNEAELIAYGLRLGLEQQTAQQILKR
                     IDQAFDVVCERLAQDARYTEDGLLLRIKQAVYALR"
BASE COUNT          397 a          306 c          246 g          403 t
ORIGIN      
        1 caacaaatgt tgttcccata aaggttcaaa aatacgacaa aaattatcga caagacagta
       61 aagttcggta agattatcca tgagcgtacc ttttgtgtgg ggctagtttt gtgtgtcgtt
      121 acccaaactc taaccctttt caaaggtacg ttccttatct ttcttatccc gaactcaggt
      181 tattttaaaa acacagtttt ataaatgctt tgattttttg gctttattaa tcaagtatct
      241 ctatcttaaa gcataaaccg cctgcttaat tcgcagtaat aagccatctt ctgtatagcg
      301 cgcatcttga gctaagcgtt cgcacactac atcaaatgct tggtcaatac gttttagaat
      361 ctgttgtgct gtctgttgtt ctaagcctaa acgcagacca taagcaatca gttctgcttc
      421 gttaggaaaa ctctttttct tatttaactt caacgccagt tgaccgtcta attcaggata
      481 aatattggta cagaccacat cgtaaatagg ggctaattgt cgtctagccg atggctgctc
      541 gtaaagtaac ccgatatttt ttaagtgcgc atcaccgtca cccaatttac aagacaaggc
      601 aacacgctca aataatcgct gtaattcaat atgactgtat tcgcttgtaa atatagccac
      661 agctttggca atcatttcgt aactgccttc atattttctg gcggtatttc tgcctgttaa
      721 tacggccata tcttcaaagc ccaacgcgga accatcggct tttcggtcaa aacgggacat
      781 gactaataac aaaccatcat cagacaacca aaaatcaggc acactgaacc ctgcttgttt
      841 agcgactgat aaacacaggt attcattgcg ggctataccg acataatctt gcccttctgc
      901 ttttacaatc acggtatcta aagccaatga gctgtgacct gcgggcaaag cctgagccaa
      961 aggcactaag gttttaggct gcacacctga tatgccagac gttaacgcat atttatcgac
     1021 taagtagata aataaatcgg tagatgatga ctgtaataaa gtttgtagtg tttcgccctt
     1081 ttcttttggc gcaatttggc catcaagtgc atagtttaag cgaccaattt gatgtgcacc
     1141 tgctaaataa agcaaaaaca tatcactggg cgcacctaag cgtttcagcc gctcttcaac
     1201 catgtagcgt aagtagcctt caggccgatt catcgaaaat atgcccatca actcaccact
     1261 acaatagctt tgttgacgga caggcatcac taaagataca gctgtttctg tttccgcgtt
     1321 aaggccatag ttaaacacaa agttagattc tt
//
LOCUS       DAOQMB010000635         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82906_length_1350_cov_15.694208, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000635 DAOQMB010000000
VERSION     DAOQMB010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82906_length_1350_cov_15.694208"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLY05_12750"
     CDS             complement(<1..818)
                     /locus_tag="PLY05_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04208.1"
                     /translation="MANYMAEWNTQQALPESFLALAQQIYQNDPHWLGEDEASLRAQF
                     SAQNTWFADGKAWLGVIENQARVVGFVNDKQVVDGEKAAFFGFWESTDNLAANQQLFA
                     ELSAWAKTQGCTRLYGPINFSTFSANRIRVDAFEYGAFVGEPWNPPYYRQLLEGLGYQ
                     ERYSYLSTFSPLEEIYEFVANIYLPIKGFVEQKFVFESMTPEFWLSNLETMYGFVNNV
                     FRENFAYTPIDYNTFERLCGESFAHKFCPKTSILAKTHEGDIAGFFLVYPDYSS"
     gene            complement(811..>1350)
                     /locus_tag="PLY05_12755"
     CDS             complement(811..>1350)
                     /locus_tag="PLY05_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNC04209.1"
                     /translation="DIFYLSLEEIYAWYDGRAVQTNIKGLTQLRKAEYQSYEQQDLPH
                     HFWTYGVVYHHNSYQYPYQENIQLDGDLQGTGCYPGVVENKIRLIFSPDDELSLNGQI
                     LCTVRTDPGWAPLFPTAGGILVERGSTLSHSAVVARELGIPAIVGIPNITKILHDGEL
                     VKMDGALGTIIRLEEQNHG"
BASE COUNT          410 a          292 c          265 g          383 t
ORIGIN      
        1 aagctactgt aatcaggata gactaaaaag aagcctgcaa tatcaccttc gtgtgttttt
       61 gctaaaattg aggtctttgg acaaaattta tgggcaaaac tttcgccgca taagcgttca
      121 aaagtgttgt aatcaatagg ggtgtaggca aagttttctc taaacacatt attgacaaaa
      181 ccatacatgg tttctaagtt gcttagccaa aactcaggtg tcatgctttc aaagacaaat
      241 ttttgctcaa caaagccttt aatcggcaaa taaatattgg caacaaattc ataaatttct
      301 tcaagtgggc taaacgttga taaataacta tagcgctctt ggtaacctaa accttctaat
      361 aattgacggt aatagggggg attccacggt tcaccgacaa acgcaccata ttcaaaggca
      421 tcaacacgaa tacgattggc ggagaaagtg ctaaagttaa ttggcccata taaacgagtg
      481 caaccttgtg tttttgccca tgcacttaat tcagcaaaca attgttggtt tgcagctaag
      541 ttgtcggtac tttcccaaaa accaaaaaat gcggcttttt cgccatcaac cacttgtttg
      601 tcattaacaa agccaaccac gcgtgcttgg ttttcaatca cacctaacca tgctttgcca
      661 tcagcaaacc atgtattttg tgcactaaat tgtgcgcgta agctggcttc atcttcgccc
      721 aaccaatgtg gatcgttttg ataaatttgc tgtgctaaag ccagaaaact ttctggtaac
      781 gcctgttgag tattccactc agccatatag ttagccatga ttttgctcct ccaaacgaat
      841 aattgtgcct aaagcaccgt ccattttgac caattcacca tcgtgtaaaa ttttagtaat
      901 gttcgggata ccaacaatgg caggaatacc taattcacga gcgacaaccg ctgaatgtga
      961 cagggttgaa ccacgctcaa ccaaaatgcc gcctgcggtt ggaaacaagg gcgcccaacc
     1021 cggatcggta cgcaccgtac ataaaatttg gccattgagc gacaattcgt catcaggcga
     1081 aaaaatcaaa cgtattttgt tttcaacaac accagggtaa caacctgtgc cttgtagatc
     1141 accatcaagt tgtatgtttt cttgataggg atattgataa ctgttgtgat gatagaccac
     1201 gccatacgtc caaaagtggt gtggcaagtc ttgttgctca tagctttgat attcggcttt
     1261 gcggagttgg gttaaacctt tgatgttggt ttgcaccgca cggccatcat accaagcata
     1321 gatttcttct aagctgagat aaaaaatatc
//
LOCUS       DAOQMB010000636         1350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_82907_length_1350_cov_11.861004, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000636 DAOQMB010000000
VERSION     DAOQMB010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82907_length_1350_cov_11.861004"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..>1350
                     /locus_tag="PLY05_12760"
     CDS             16..>1350
                     /locus_tag="PLY05_12760"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNC04210.1"
                     /translation="MEIKAYFTEIHKVIIKELEAATVQINVAVAWFTDKEIFDVLCKK
                     AQSGVKVFVVLMADDINCGAGRLNFTRLENLGGKMTFLPASSQDVPLMHHKFCVIDGK
                     TVLTGSYNWSKKARSNDENITVVWDAAEFAYKYLNVFEELCLRVGQQATSLISAESVK
                     RRLEMIRHLILLGDYEDVTAQVIKLNPAANEYKLTPIIEELNGGKYQEALEKINSYLS
                     KFTALVSLEAQEIPRLQFELQILELRLESLSNEKAELERCLIVFNRRHDEVLGDLIKN
                     VLQARAEYARLMFDMKQKELEQEAIQSEKVQEEAKAAEEKAEEAEHTYQQYSQQHEQL
                     QYEQAIPELDAQQEKELKTLYRKACSLCHPDKVPEDKKQLAHEIFVELQEAYKSNDIE
                     QVANIYERVKKGDFKQTRASTLTKTEILKAAIAEIKYQLDKLLTELQVLQTSQ"
BASE COUNT          474 a          209 c          301 g          366 t
ORIGIN      
        1 cagagactaa acaatatgga aattaaagcc tattttacag aaattcacaa agttatcatt
       61 aaagagttgg aagcggctac tgtgcaaata aatgttgcgg tggcatggtt tacagacaaa
      121 gaaatttttg atgttttatg caaaaaagcc caaagtggag ttaaagtttt tgttgtactc
      181 atggctgatg acattaactg tggcgcaggt cggcttaatt ttacacgcct tgaaaattta
      241 ggcggaaaga tgaccttttt gcctgcaagt agccaagatg taccactcat gcaccacaaa
      301 ttttgtgtga ttgatggcaa aacggtgcta acaggctctt ataattggag taaaaaagca
      361 cgtagtaacg atgaaaatat tacggttgtt tgggatgcgg cagaatttgc ctacaaatac
      421 ttaaatgtgt ttgaagagtt gtgtttgcgt gtggggcagc aagctacttc tttaatcagt
      481 gcggagtcgg taaaacgccg cttggagatg attcgtcatt taattttatt gggcgactat
      541 gaagacgtga cggctcaagt tattaaactc aatcctgctg caaacgaata taaattaacg
      601 cctattattg aggagttaaa tggcggtaaa tatcaagaag ccttagaaaa gataaatagt
      661 tatttaagca aatttacggc attagtatcg cttgaagcac aagaaatacc acgcttacaa
      721 tttgaattac agattttaga gttacgttta gagtcattaa gcaatgaaaa agcagagtta
      781 gaacgctgtt taattgtttt taataggcgg catgacgagg tattaggcga tttaattaaa
      841 aatgtgttac aggctcgtgc cgaatatgcg cgtttaatgt ttgacatgaa acaaaaggag
      901 cttgagcaag aagctattca aagtgaaaag gtgcaagaag aggccaaagc agccgaggaa
      961 aaagccgaag aagccgaaca tacttatcag cagtattcgc aacaacatga acaattacag
     1021 tatgagcaag cgattcctga attagatgcc caacaagaaa aagagttaaa aaccttatat
     1081 cgtaaagcgt gtagtttgtg ccaccctgat aaagtgccag aagataaaaa gcaacttgcc
     1141 catgaaattt ttgttgagtt gcaagaagcg tataaaagta atgatattga gcaagtggct
     1201 aacatttatg agcgtgttaa aaagggtgat tttaagcaaa cacgcgcaag cactttaact
     1261 aaaaccgaga tattaaaggc agcgattgcc gaaattaaat atcagcttga taagttgcta
     1321 actgagttac aggtattaca aacaagtcag
//
LOCUS       DAOQMB010000637         1349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_83011_length_1349_cov_7.496136, whole genome shotgun sequence.
ACCESSION   DAOQMB010000637 DAOQMB010000000
VERSION     DAOQMB010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83011_length_1349_cov_7.496136"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..326
                     /locus_tag="PLY05_12765"
     CDS             42..326
                     /locus_tag="PLY05_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04211.1"
                     /translation="MLLCSNPLFSLGQTVATPHALELLAQHNISCFSLLARHQSGDWG
                     NVPVEDALSNQQAIECGYRIMSVYPLETGKVWIITEADRSVTTVLLPEEY"
     gene            complement(404..646)
                     /locus_tag="PLY05_12770"
     CDS             complement(404..646)
                     /locus_tag="PLY05_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04212.1"
                     /translation="MAITKPIPKKESVDQFINNAPDGDKLRKGVKKGKREQISLTIPP
                     ALLDRLDAVANRLALSRAGMINLAIVRAIEQEEKNN"
     gene            complement(685..1320)
                     /locus_tag="PLY05_12775"
     CDS             complement(685..1320)
                     /locus_tag="PLY05_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC04213.1"
                     /translation="MILTVGNTKGGVGKTTLALNIAIARAIAGRDVWLIDGDRQGTAQ
                     TAIALRADAGREPAIACAQYADGSALRAQLQHQSKKFEDIIIDAGGRDSTALRAALVL
                     SDVLLVPFAPRSLDVWALADICALIDEARSVRDGLRAYAVLNCADTHGTDNQEAAEAV
                     ADYPQLVYLNTPIKRRKAIANAAGQGLSVLEAIPKDVKANDELNALMLRLF"
BASE COUNT          404 a          297 c          221 g          427 t
ORIGIN      
        1 cttcatcaac taattaactt taacctacgc ggagaatcat catgttactt tgcagcaatc
       61 ctttattttc gctcggtcaa acagtggcca caccccatgc ccttgaactg ttagctcaac
      121 acaacatttc ttgctttagc ttattggctc gtcatcaatc aggagattgg ggcaatgtac
      181 ctgtggaaga tgcgttaagt aatcaacaag cgattgaatg tggttaccgt attatgtctg
      241 tttatccttt agaaacaggc aaagtgtgga ttattaccga ggctgatagg tcggtgacta
      301 cggtattgtt gccagaggag tattagtatt tacacactta gtagctatca ttttaccatt
      361 catttaatat taaatgaatg gtaaaatgat agctacaagt tgactaatta tttttttctt
      421 cttgttcaat tgccctaaca attgctaagt taatcatgcc tgcacgagat aaagctaaac
      481 ggttagcaac agcatctaaa cggtctaata aggctggagg aattgttaaa ctaatttgct
      541 ctcgctttcc tttttttaca cctttgcgca gcttatctcc atcaggagca ttattgataa
      601 actgatcaac actttccttc tttggaatag gttttgtaat agccatgtaa taccttttta
      661 atatgaaaat aattattttt aatattaaaa caatcttaac attaaagcat taagctcatc
      721 attagctttg acatcttttg ggattgcctc taagactgat aaaccttgcc cagcagcatt
      781 ggcaatagcc ttacgacgct taatcggggt atttaaataa actaattgag gatagtccgc
      841 tacagcctca gccgcctctt gattatcagt tccatgagta tctgcacaat taagtacagc
      901 ataagctctt aaaccatccc tcactgatct agcctcatca atcaacgcac aaatatccgc
      961 taatgcccaa acatctaatg atcgaggtgc aaaaggaaca agcaatacat cggataatac
     1021 taatgcagct ctcaaagctg ttgagtctcg tccacctgca tcaataataa tatcctcaaa
     1081 ctttttactt tgatgctgta attgcgccct taaagcagac ccatccgcat attgcgcaca
     1141 agcaatagct ggttcacgac cagcatctgc acgaagagca attgcagttt gagcggttcc
     1201 ttgtctatca ccatctatta accatacatc tctacctgct atcgctctag caatagcaat
     1261 atttaacgct agagtagtct tgcctacacc acctttagta ttaccgactg taagtatcat
     1321 aaaacacctt ttttacacct tttttaata
//
LOCUS       DAOQMB010000638         1343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_83786_length_1343_cov_10.394410, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000638 DAOQMB010000000
VERSION     DAOQMB010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83786_length_1343_cov_10.394410"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            328..774
                     /locus_tag="PLY05_12780"
     CDS             328..774
                     /locus_tag="PLY05_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04214.1"
                     /translation="MIEQLTAQTLHEVLPLIAQYQAFYQVADINSQRNEQFFSQFVNH
                     SPLGALFLYRQNGKVIGFATVYFSFSSTITAKVGIMNDLFTLEDYRGQGIGKALINHC
                     LDYALNQGAARLQWVTALDNTQAQALYNSLDTHKKPWLFYTYAPKT"
     gene            complement(771..1244)
                     /locus_tag="PLY05_12785"
                     /pseudo
     CDS             complement(771..1244)
                     /locus_tag="PLY05_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT          388 a          276 c          257 g          422 t
ORIGIN      
        1 tataaatggc aaaagaccag ttatgcgcca ttgcatcaac aggggacatg cagggaactc
       61 ctagcactca aagcggggaa attgattggt ttgcacaaga tgaatgattg cgtttgaaca
      121 cgacttctgg tttatgttca aacactcgaa tcaaatcagc tttgttacat acaattacat
      181 atattgtact acagttatca ttctatgttc taggggcgta gattaagact ttttgataga
      241 aacaaaattg tctatcaaca aagacttatt ggctatgtat ttgttaacat tattcccctc
      301 aaccatcaaa attaaggata tatgcccatg attgagcaac ttaccgccca aacactgcat
      361 gaagtattac cactcatcgc ccaatatcaa gctttttatc aagttgctga tattaattca
      421 caacgcaatg agcaattttt tagtcaattt gttaatcata gtcctttagg tgctttgttt
      481 ttatatcgac aaaatggcaa agtcattggt tttgcgacgg tttattttag tttttcgtca
      541 actattacgg ctaaagttgg cattatgaac gatttattta ccttggaaga ttatcgcggc
      601 caaggcattg gcaaagcctt aattaatcat tgcttagatt atgctttaaa ccaaggagca
      661 gcacgtttac agtgggtgac tgccttagat aacacacaag cacaagcctt atataattct
      721 ttagacaccc ataaaaaacc ttggttattt tatacttatg cgccaaagac ttaggctgac
      781 tgctgaacaa tttgataaat ttggctatat gaacgctctt ttttaatctg ttgaaagagc
      841 gtttgtgctt ctggccatgc ttgcagcaac atccccaacc attgtttata acgacctact
      901 gcacctgctt ctgtccattt ttgttggcta tccaccacta aaggattaac cactgcctgt
      961 tgcatgtgct gcaaaaattg tctttgccaa tcaagtaatt gtgcccacgt taattgatct
     1021 gtctcgcctc gcaacgcacg aattaaatag ggctggatta ctgctgctcg gccaatcatg
     1081 atatccgtgc attgcgattg ttgttggcat cgctgagcat tcgttaccga atcaatgtca
     1141 ccattagcaa tcatggggag tttaacgtgt tgacgaatgg tagctaattc atgccaatgg
     1201 gcgggagggc gatagccgtc agctttggta cgggcatgaa tcgttagcca actagccgtt
     1261 ccataaaact tatcaaaacc tacttattat agaacaaata ggctttgtgt ttacttcctg
     1321 cttcaacaag attgcccaga ggg
//
LOCUS       DAOQMB010000639         1341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84045_length_1341_cov_17.857698, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000639 DAOQMB010000000
VERSION     DAOQMB010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84045_length_1341_cov_17.857698"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLY05_12790"
     CDS             complement(<1..509)
                     /locus_tag="PLY05_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HNC04215.1"
                     /translation="MAIKDWPNAERPREKLLEQGAESLSDAELLALFLRTGTVNKSAV
                     DIGRDLIKQFGSLRCLLEAPTEQVLAQEGIGEAKYSLLQASLELGRRYLAQSLAEKEL
                     LTSPQMVKSFLRAQLRHHQQELFAALFLDNQNRLIRFEILFTGTLDSCAVHPREVLKR
                     ALALHSASL"
     gene            653..>1341
                     /gene="coaBC"
                     /locus_tag="PLY05_12795"
     CDS             653..>1341
                     /gene="coaBC"
                     /locus_tag="PLY05_12795"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017392408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HNC04216.1"
                     /translation="MLATLKHKRVILAVSGGIAAYKSADLVRRLKDAGADVHVVMTAA
                     AQQFITPLTMQALSGNPVHTELLDSAAEAGMGHIQLARWADLILVAPASADVLARLAQ
                     GMANDLLTTLWLATRAKRAVAPAMNQQMWAASITQENLAKLAQQGVTIFSPASGSQAC
                     GDVGMGRMPEAVDLAQRCADLFEVGTLTGKKVVITAGPTQEALDPVRYITNHSSGKMG
                     FCLAEACVEAGA"
BASE COUNT          382 a          248 c          314 g          397 t
ORIGIN      
        1 attaaactcg ctgaatgtag tgctaaggcg cgtttgagga cttcgcgagg atgcacagca
       61 cagctatcta aagtacctgt aaacagaatc tcaaaacgaa tcagacgatt ttgattatct
      121 aaaaataagg cagcaaataa ctcttgttga tgatgccgta attgggcacg taaaaaactt
      181 ttgaccattt gtgggctggt taataattct ttttcggcta aagattgagc cagataacga
      241 cgacctaatt ctaaagaagc ttgtaaaagg gaatatttag cttcgccaat accttcttga
      301 gccaagactt gttcagtagg agcttctaat aagcaacgta aggagccaaa ctgtttaatc
      361 agatctcggc caatatcaac ggcagattta ttgacagtac cagtgcgtaa aaataaagct
      421 aataactctg catctgataa agattcagcg ccttgttcta aaagtttttc tcgggggcgt
      481 tcggcatttg gccagtcctt gattgccata aaaatcatcc taatttgtaa aacctgtgac
      541 attctataag aaaaatgact ttttactagc cacaagttca taaaactatg tttcgctggt
      601 ctttgattat ggtatcttta tcggctgatt taatgatgtt taagaacgaa agatgttggc
      661 aaccttaaaa cataaacgtg ttatcttggc tgtgagtggt ggaatcgctg cttataaaag
      721 tgcggattta gttcgccgtt taaaagatgc tggtgcagat gttcatgtgg tgatgacagc
      781 cgcagcacag caatttatta cgcctttaac tatgcaagcg ttgtcgggaa atcctgttca
      841 tactgaattg ttagattctg ctgctgaggc aggcatggga catattcaat tagcgcgttg
      901 ggctgattta attttggttg ctccagccag tgccgatgtt ttggcgcgtt tggcacaagg
      961 tatggccaat gatttattaa ccacattatg gttggcgaca cgcgcaaaac gcgctgttgc
     1021 ccctgcaatg aaccaacaaa tgtgggcagc aagtattact caagaaaatt tagctaaatt
     1081 agctcagcaa ggagtgacga tattttcacc tgcttctggt agccaagctt gtggtgatgt
     1141 aggtatgggt agaatgcccg aagcagttga tttagcgcag cgttgtgctg atttgtttga
     1201 ggtgggcact ttaacaggca aaaaggttgt gattaccgca gggccaaccc aagaagcctt
     1261 agatcctgtg cgttatatta ctaatcatag ttctggtaaa atgggctttt gtttggccga
     1321 agcctgtgtt gaggcaggtg c
//
LOCUS       DAOQMB010000640         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84149_length_1340_cov_18.797665, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000640 DAOQMB010000000
VERSION     DAOQMB010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84149_length_1340_cov_18.797665"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /gene="mnmC"
                     /locus_tag="PLY05_12800"
     CDS             <1..1182
                     /gene="mnmC"
                     /locus_tag="PLY05_12800"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HNC04217.1"
                     /translation="VIIGAGIAGVSCARALATRGWQVTIIDKANDAMQAASGNPAAII
                     YPKLAPAHLSAWHFQQQAYLWLLNLLKTDDYQHIWCQTGLLWLLVGNQLREGAKLNDH
                     PWPTDLVQKVSPQQASQLSGVTIACECLFFPQAGFLQPQALAQTWLNHPNITCLWQHD
                     VADICYQNNQWLILDQQQQTICQAPVVIISNALAAQQLTPTAHLPLTPVRGQIAHFTT
                     ETPLQQLKTVLCYGGYLTPAYGQSPQRHCLGASFWPRNKDSQVTNEDHQHNQQLLQQF
                     LPHLAKQLPSIDTWQGRAALRAQTSDYLPMVGALPVYEDFIERYAAFKHGRTVNEAPN
                     YHQGLYVSLGHGSKGFCYAPLAAEVIAAQLNNEPMPIAKRVLEALHPARFWVKQIKRQ
                     R"
BASE COUNT          393 a          301 c          262 g          384 t
ORIGIN      
        1 gtgattattg gtgcgggtat tgctggtgtc agttgtgcac gggcattggc cacacgtggt
       61 tggcaagtta ccattattga taaagccaat gatgccatgc aagctgcttc gggtaatcct
      121 gcggcaatta tttatcccaa acttgcacct gcccatttat cggcatggca ttttcaacaa
      181 caagcctatt tatggttatt aaatctatta aaaaccgatg attatcaaca catctggtgt
      241 caaacaggtt tgttatggct gctcgttggc aatcaattgc gtgaaggagc aaaattaaac
      301 gatcatccgt ggccaactga tttagtgcaa aaagtttcgc cacaacaagc cagtcaatta
      361 tcaggcgtta caattgcttg cgagtgttta ttttttcctc aagcaggttt tttgcagcct
      421 caagctttag cacaaacgtg gctgaatcat ccaaatatca cctgtttatg gcaacacgat
      481 gtcgccgata tttgctacca aaataatcaa tggttaatcc tcgaccaaca acagcaaacc
      541 atttgccaag cacctgtggt tattataagc aatgctttag cggctcaaca attaaccccg
      601 actgcgcatt tacctttaac gccagttcgt ggtcagattg cccatttcac cacagaaact
      661 cccttacaac agctaaaaac cgtgttgtgt tatggtggtt atttgactcc tgcctatggc
      721 caatcaccac agcgccattg tttgggggca tctttttggc cacgcaataa agatagccaa
      781 gtcacaaatg aagatcatca acataaccaa cagcttttac agcagttttt accccattta
      841 gccaagcaat taccctctat agacacatgg caaggtcgtg ccgcattacg cgctcaaacg
      901 agcgactatt tacccatggt gggtgcgtta cctgtttatg aggattttat cgagcgttat
      961 gcggcgttta aacatggtag aacggttaat gaagcgccta attatcatca aggtttgtat
     1021 gtatctttag gtcatggttc taaaggattt tgttatgcac cgttggctgc cgaagtcatt
     1081 gctgctcaac tcaataatga gccaatgccc attgctaaac gggtcttaga ggcgttgcat
     1141 cctgcacgtt tttgggtaaa acaaataaaa agacaaagat aatctagcac catcgtacaa
     1201 attggcatca atagtaaaaa cagatcaaaa aagcttatct ataatgataa aaaatcattt
     1261 taggtcaaca acaccatgtc acaacctctc gatgccaaac aagcattaat catcaatggt
     1321 caacctcgct ttggttattt
//
LOCUS       DAOQMB010000641         1340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84153_length_1340_cov_8.591440, whole genome shotgun sequence.
ACCESSION   DAOQMB010000641 DAOQMB010000000
VERSION     DAOQMB010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84153_length_1340_cov_8.591440"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1180)
                     /gene="aroC"
                     /locus_tag="PLY05_12805"
     CDS             complement(89..1180)
                     /gene="aroC"
                     /locus_tag="PLY05_12805"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004995323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNC04218.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGAIVDGVPPNLSLTEADLQLD
                     LDRRKPGTSQFATQRKEEDQVKILSGVFEGKTTGTPIGLLIENTDQKSKDYGNIATTF
                     RPGHADYTYTQKYGFRDYRGGGRSSARETAMRVAAGGIAKKYLFEKFGIVIRGFVQQI
                     AHIKATELDWDYVNQNPFFCGDKNVVPQFEELITQLRRDGTSCGARLCVVADNVPVGW
                     GEPVFDRLDADIAHAMMSINAVKAVEIGDGFAVVEQFGHESRDELTPQGFTANHSGGV
                     LGGISSGQQIVVSIALKPTSSITTAGKSIDLDGNPIEMLTKGRHDPCVGVRATPIAEA
                     MLAIVLMDHFLRHRAQNADVVMPLKPIAP"
BASE COUNT          377 a          331 c          264 g          368 t
ORIGIN      
        1 tctcttgcgt tgcgtgtgct tggatgtttc cctccttttc gccttagaca cacgatcctc
       61 ctcctcgcaa ctagagggct ttttattttt acggtgcaat cggttttaac ggcataacta
      121 cgtctgcatt ctgcgcacga tgacgcaaaa agtgatccat taacacaatc gctaacatcg
      181 cttcagcaat cggtgtagca cgcacaccca cacaagggtc atgacgacct ttggtcagca
      241 tttctatcgg attaccgtct aaatcaatac ttttacctgc ggtggtaata ctggatgtgg
      301 gttttagcgc aatcgagacc acgatttgtt gaccgcttga aatccctcct aacacaccac
      361 ccgaatgatt agcagtgaag ccttgtggtg ttaattcatc acgcgactca tggccaaact
      421 gttcaacaac cgcaaaacca tcaccaattt cgaccgcttt caccgcatta atgctcatca
      481 tcgcatgggc aatatcggca tctaaacgat caaatacagg ctcaccccaa cccacaggca
      541 cattgtcggc aaccacacat aaacgtgcgc cacagcttgt gccatcacgg cgtaattgag
      601 taattaattc ttcaaattga ggcacaacgt ttttgtcacc acaaaagaaa ggattttgat
      661 tcacataatc ccaatctaat tcagtggctt taatatgggc gatttgttgc acaaaaccac
      721 gaatgacaat gccaaacttt tcaaataaat attttttggc aataccacct gcggcaacac
      781 gcatcgctgt ttcgcgcgca gaagaacgac caccacctcg ataatcgcga aagccatatt
      841 tttgggtata ggtataatcg gcatggccgg gtctaaacgt tgtagcgata ttgccgtaat
      901 ctttagactt ttgatcggtg ttttcgatta ataaaccaat cggtgtacct gtggttttgc
      961 cttcaaacac acccgataaa attttaactt ggtcttcttc tttacgttgg gtagcaaact
     1021 gtgacgtacc gggtttacgg cggtctaaat ccagttgcaa gtcggcttcg gtgagtgaca
     1081 aattcggtgg cacaccatcc acaatcgcgc ctaatgccaa accatgtgac tcaccaaagg
     1141 tagtgacacg aaataattga ccgatagtat tgcctgccat ttataaactc tcctgaaaca
     1201 ttagactgat gcaatgcacc gcattaaggg cgttttgcga acataaaatc gttgaaaacg
     1261 attgtaatgt gagaaaataa gccattatcg catcagtcct taaaaagctg ttgatattga
     1321 cgacattgtt ccgccgttaa
//
LOCUS       DAOQMB010000642         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84398_length_1338_cov_26.184723, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000642 DAOQMB010000000
VERSION     DAOQMB010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84398_length_1338_cov_26.184723"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..1292)
                     /locus_tag="PLY05_12810"
     CDS             complement(63..1292)
                     /locus_tag="PLY05_12810"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005344802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNC04219.1"
                     /translation="MTNITLSVQRLVAYREAIKQIKDGNCSLSFPKDTDIEPILDFES
                     EFIQLAAWLDVRFDEFNKIQEISTEINKGTVLDDVLNRIYDTFHIIIPYDRISYALIS
                     DDHEKVIAHWTKTNYPEKTRIKKGYTASLAGSSLEKILTTQQPRILNDLHLYLANNPK
                     SLSTQLIVSEGIQSSLTCPLIADDKPIGFIFFSSKEKNTYIDAHQKVFISIARQISVL
                     VEKSRLYQHIYDLNNQLITALSQLKEQSSRDALTSIFHRGAIMEFLKNTLEKDARKNQ
                     PTTVILADIDHFKHINDTYGHITGDTVLKEVSKSLTSHLRSYDCVGRYGGEEFLIILG
                     ETNTTDALLITERIRQAISELKFERDNDRFSLTMSFGVSCSDNILHKKTEETLLLEAD
                     SALYHAKNDGRNRVCIA"
BASE COUNT          394 a          233 c          242 g          469 t
ORIGIN      
        1 taattaaccg gcggcacgta gtgccgtcca gtgagcgaat gcgaacgagg ttgaattatt
       61 tgttatgcta tacacactcg atttctaccg tcattcttcg catgatataa agcactatct
      121 gcttctagaa gtaatgtttc ttctgttttt ttatgtaaaa tattatctga acaggaaacg
      181 ccaaagctca tggtgagaga gaatctatca ttatctctct caaatttgag ctcagagatc
      241 gcttggcgaa tacgctctgt aattagcagc gcatctgtag tattagtttc acccaatata
      301 attaaaaatt cttcaccgcc gtaacgccca acacagtcat aacttcttaa gtgactagtt
      361 aaacttttac ttacctcttt cagaactgta tcaccagtga tatgaccata tgtgtcattt
      421 atatgtttga aatgatctat atcagcaagt attacggtgg taggttgatt ttttcttgcg
      481 tctttttcga gagtgttttt taaaaattcc atgatggcac ctcgatgaaa gatgctagta
      541 agtgcatcac gagaagattg ctctttgagt tgcgataacg cagttatcaa ctggttattt
      601 aagtcataaa tatgctgata aaggcggctt ttttcgacta aaacagatat ttgccttgca
      661 atactaataa ataccttttg gtgagcgtca atgtatgtgt tcttttcttt gctagaaaag
      721 aaaataaatc caataggctt atcatctgca ataagtggac aggttaaact tgattgaata
      781 ccttcagaaa ctatcaactg agtggacaaa gactttgggt tattagctaa atataagtgt
      841 aggtcattaa gaatgcgggg ttgttgtgtt gttagaattt tctccaaact actccccgct
      901 aatgatgcag tgtatccttt ttttattctt gttttttctg gataattggt ttttgtccaa
      961 tgagctataa ctttttcatg atcatcagat attaatgcgt agctgattct gtcataaggt
     1021 attattatat gaaatgtatc ataaatgcga tttagaacat catcaagtac agtgcctttg
     1081 ttgatttcag tggatatctc ttgaatttta ttgaactcat caaagcgaac atcaagccac
     1141 gcagccaact gaataaactc agattcaaaa tcaagtatag gttctatatc agtatcttta
     1201 ggaaaagata atgaacagtt gccatctttg atttgtttta ttgcttctct gtatgcgact
     1261 aatctttgaa ctgatagcgt tatattggtc ataccttatc cttgttggga tagccaaatt
     1321 ctcttatagc ataacgcc
//
LOCUS       DAOQMB010000643         1338 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84450_length_1338_cov_3.399844, whole genome shotgun sequence.
ACCESSION   DAOQMB010000643 DAOQMB010000000
VERSION     DAOQMB010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84450_length_1338_cov_3.399844"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..702)
                     /locus_tag="PLY05_12815"
     CDS             complement(79..702)
                     /locus_tag="PLY05_12815"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNC04220.1"
                     /translation="MSFSYKYLLLSLSLAVGSVWAIEQKLSIEEIIQQAEQGAAESQY
                     QLAIKYELGDHLQQSYEQAVVWLTKAAQQNHIEAQLKLGMMYFNGQGVARNNVEAAEW
                     FRKAAYLKQGNKPAYYAKALIPVDGVAKALWWLERVAEQKVSQAQYQLAQIYAQTDVV
                     KALTWATIANTLGYQPAKTLLSQLSTQSNSQQQQQAEQLANDWLNSH"
     gene            complement(714..1184)
                     /locus_tag="PLY05_12820"
     CDS             complement(714..1184)
                     /locus_tag="PLY05_12820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04221.1"
                     /translation="MMHETPNDPRISLLYQSLAKQQVTPTIEQHILQMAEQQANINRT
                     TQGKSNAVWQKISSLFSHSWQGGLAFAGVVTVICAIVFNTLQPSDNIYSTKEIIPPTE
                     AELQAQTWAELRTLIAQKKYDEAREQLNNIRQRNPQVVVPENITQALDTSTKTP"
BASE COUNT          346 a          242 c          279 g          471 t
ORIGIN      
        1 tggcattgcc attgcctagt ttggagcatt gtttgcagca aacggcaaaa tttgtcgcta
       61 ggcaatagct cctaacaatt aatgtgagtt taaccaatca ttggctaatt gttctgcctg
      121 ttgttgttgt tgtgaattgc tttgtgtgga tagttgtgat aacaacgttt tagcaggctg
      181 atatcctaat gtattggcaa ttgttgccca tgttaacgct ttaactacat cggtttgggc
      241 ataaatttgg gctaattgat attgtgcttg agaaactttt tgttcggcaa ctcgttccag
      301 ccaccataag gctttagcaa caccatcaac aggaattaat gcttttgcat aatatgcagg
      361 tttattacct tgttttagat aagctgcttt acgaaaccat tcggctgctt cgacattatt
      421 acgagcaact ccttgaccat taaagtacat catgcctaat ttcagttggg cctctatatg
      481 attttgttgg gctgctttgg ttaaccaaac aacagcttgt tcataacttt gttgtaaatg
      541 atcgccaagc tcatacttga tagctagttg atattgagat tcagcagcac cttgttcggc
      601 ctgttggata atttcttcaa tagagagttt ttgttcaatt gcccataccg aaccaacagc
      661 cagacttaaa cttagtaaaa gatatttata agaaaaactc atgatgttga cctctatgga
      721 gttttggttg acgtgtctaa tgcttgagta atattttcag gaacaactac ttgtggatta
      781 cgttgacgaa tattgttgag ttgttctcgg gcttcatcat attttttctg ggcgattaat
      841 gtacgtagct ctgcccatgt ttgcgcttgc aattctgctt cagtgggagg aatgatctct
      901 ttagtgctat aaatattgtc tgagggttgg agtgtgttaa aaacgatagc gcatattact
      961 gtaactacac ccgcaaatgc taaaccacct tgccaagagt gcgaaaagag agagctaatc
     1021 ttttgccaaa cagcgttgga ttttccttgt gtagttcgat tgatgttggc ttgttgttca
     1081 gccatttgca aaatatgttg ttcaatcgtc ggcgtaactt gttgcttagc taaactctga
     1141 tataacaggc tgatccttgg atcattaggc gtttcatgca tcattatcgc ttctcaacag
     1201 ctaaactaat atgttaaagg ttttccgtta ataattgtgt ttgccattgt tgattgtcta
     1261 aacgttgatt tataggttgt ttaaattcgt aatgttgcca aagtgcgcca cgagcttgtt
     1321 gttgctcaat gactctag
//
LOCUS       DAOQMB010000644         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84533_length_1337_cov_17.968019, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000644 DAOQMB010000000
VERSION     DAOQMB010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84533_length_1337_cov_17.968019"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="PLY05_12825"
     CDS             complement(<1..>1337)
                     /locus_tag="PLY05_12825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HNC04222.1"
                     /translation="EKIHAAAFAPTPLARQTSPAQVVQDFATVRQLVTGSFVLALLDL
                     PWLPIYIGLLFAFEWPLGLLAITGIVLLTSIAVITEKLSKQPLQESSKIASQAAFMLN
                     KQHRNIDIISALGMIPVLGKRWQAHNQHALLLQMQASDKMAQLSAIGRYIRLTLQSLV
                     LGIGAYLVLEGQMSGGMMIAGSILLGRALAPVEQLIASWKQFMQGMEANQRLQQLLQA
                     NQQQQQQAFDLASPTGQMSIENVSLALEGRKEPVLQQINFKLNAGQILAVVGASASGK
                     TTLARLLLGIVQPTSGEVRLDNADLRQWSRDKLGKYMGYLPQDIELFEGTIAENIARF
                     QEVDDSQIIAAAQLAHVHDMIVRLPQGYQTVIGEGGLNLSGGQRQRIALARAVFGQPK
                     LVILDEPNSNLDDQGDAALAQTLVDLKQQGTTVIVISHRTHLLNVVDQMLLLQ"
BASE COUNT          384 a          298 c          268 g          387 t
ORIGIN      
        1 tcttgtaata acaacatttg gtcaactaca ttgagcaaat gcgttcggtg tgaaattact
       61 attaccgttg taccttgttg ttttaaatca actaaggttt gtgctaaagc ggcatcacct
      121 tgatcgtcta aattggagtt tggctcatct aaaatcacta atttaggttg accaaatacg
      181 gcgcgtgcta aagcaatacg ttggcgttgt ccacccgata aattaagtcc accttcacca
      241 atcaccgttt gataaccttg gggtaaacgc acaatcatgt cgtggacatg agctaattgg
      301 gcagcagcaa taatttgact atcatctact tcttgaaaac gggcaatatt ttcggcaatt
      361 gtgccttcaa ataattcaat atcttgtggc aaatagccca tatatttgcc caatttatca
      421 cgcgaccatt ggcgtaaatc ggcattatcc aaacgtactt cgcctgatgt tggttggact
      481 atacctaaca ataatcgtgc taaggtggtt ttgcccgaag cacttgcgcc aaccacggcc
      541 aaaatttgtc ctgcatttag cttaaaattg atttgttgca atactggctc tttgcggcct
      601 tctaaggcta aactgacatt ttctatactc atttgccctg ttgggctagc taagtcaaac
      661 gcttgttgtt gctgctgttg attcgcttgc agtaactgct gcaaacgttg attggcttcc
      721 atgccttgca taaattgttt ccatgaggca atcagttgtt cgacaggggc taaagcgcgg
      781 cctaataaaa tagaaccagc aatcatcatg ccgcccgaca tttgcccctc taacaccaaa
      841 tacgcgccaa tacctaaaac caaagattgt aaagttaaac gaatataacg acctatagca
      901 ctgagttgcg ccattttgtc gctagcttgc atttgcaata acaaagcatg ttgattatga
      961 gcttgccacc gtttgccaag cacaggaatc atgcctaaag cggagataat atctatattt
     1021 cgatgttgtt tattgagcat aaatgcggct tggctagcga ttttactcga ctcttgcaag
     1081 ggctgtttac tcagtttttc ggtaatcacc gcaatactgg ttaacaatac aatacctgta
     1141 atcgccagta aaccgagtgg ccattcaaag gcaaataaca agccaatata aatcggcagc
     1201 cacggcaaat ctaataaggc caacacaaat gaacccgtca ctaattgacg tactgtggca
     1261 aaatcttgaa ccacttgtgc aggagacgtt tgtcgggcta aaggcgtagg tgcaaaagcg
     1321 gctgcatgaa ttttttc
//
LOCUS       DAOQMB010000645         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84534_length_1337_cov_15.614665, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000645 DAOQMB010000000
VERSION     DAOQMB010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84534_length_1337_cov_15.614665"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="PLY05_12830"
     CDS             complement(<1..989)
                     /locus_tag="PLY05_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04223.1"
                     /translation="MQRFLFIYFCIILFWAPIPLGSHRIWSASLLELLIGLLFIFWLF
                     TLNQNTLPKALINNKKLIALFAAIPLWSALQLIPLPPEIITLISPHHQNYIAANQWQP
                     LSLDPGATLYKLQKSIAYCLFFILCLALINTPSRLNTTAQVIVITGVLQAVYGVLVVL
                     GGPVFDVFGIAAAADRERITSASGTFVNRNNFAGYLEMCIALGIGLLVNQILINKDKF
                     AGVRAAIRNFLITMLSGKARLRVFLALMVVALVLSHSRGGNSAFYASLGVCAVLGLWL
                     YRKHHKSKSLALLFGSLIAIDVLILSSWFGLDTLAKRLDSTNIAYEERSFVFE"
     gene            complement(1061..>1337)
                     /locus_tag="PLY05_12835"
     CDS             complement(1061..>1337)
                     /locus_tag="PLY05_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04224.1"
                     /translation="APTVAVAAATAAAPTQASAIQTAAVQAAPSQAAAINQAVNSALG
                     QTANNNQQPGSGVFSNTSSVGTGISVGAGSTGSTGGGGGGSGSATPT"
BASE COUNT          482 a          249 c          253 g          353 t
ORIGIN      
        1 ttttcaaaga caaaggaacg ttcttcataa gcgatattgg tagagtctaa gcgtttggct
       61 aaggtgtcta aaccaaacca agagcttaaa attaacacat caatggcaat taaacttcca
      121 aataataagg ccaatgactt tgatttgtga tgtttacgat ataaccacaa accaaggact
      181 gcacaaactc caaggctagc ataaaaagcg ctatttccac ctcgtgaatg agataacact
      241 aaagcaacaa ccattaatgc caaaaataca cgtaaacgtg ctttgccact aagcatggta
      301 attaaaaagt ttcgaatagc tgctctcaca ccagcaaatt tatctttgtt gattaatatt
      361 tgatttacta ataaaccaat ccctaaggca atacacattt ctaaatagcc ggcaaagtta
      421 ttgcggttaa caaatgtgcc actagcagag gtaattcgtt ctctatcagc agcagcggca
      481 ataccaaaaa catcaaaaac agggccgccc aaaacgacta atacgccata aacagcttgt
      541 aaaacaccag ttatgacaat tacttgagca gtagtattta atcgacttgg tgtgttaatc
      601 agtgccaaac aaagtataaa aaacaaacag taggcgatgc ttttttgcag tttatacagt
      661 gttgctcccg ggtctaaaga taaaggttgc cattgattgg cagctatata attttgatga
      721 tgtggtgaaa taagagtaat gatctctggt ggtaatggga ttaattgtaa agccgaccat
      781 aatggaatag cggcaaaaag agcaatcagt tttttattgt ttataagtgc tttcggtaaa
      841 gtattttgat tgagtgtgaa taaccaaaaa atgaataata agccaataag cagttctaat
      901 aatgaggctg accaaatacg gtgactacct aaaggaatag gtgcccaaaa taaaataata
      961 caaaaataaa taaataaaaa acgttgcata taactaaaag aattttaggt tatagaaaat
     1021 aaaaaagggt ctgtgtagac cctttttggt aaaaagctta ttaagtagga gtggcactac
     1081 ctgagccacc accaccacca ccagtgctac cagtactacc agctccaaca ctaataccag
     1141 ttccaacact actagtatta ctaaatacgc cagaacctgg ttgttggtta ttattggctg
     1201 tttgacctaa tgcagaatta acagcttgat taattgcagc cgcctgactt ggagcagctt
     1261 gaacagcagc agtttgaata gcagaagctt gagttggagc ggcagcagta gcagctgcaa
     1321 cagctactgt aggtgct
//
LOCUS       DAOQMB010000646         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84535_length_1337_cov_10.808892, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000646 DAOQMB010000000
VERSION     DAOQMB010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84535_length_1337_cov_10.808892"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..803
                     /gene="hda"
                     /locus_tag="PLY05_12840"
     CDS             96..803
                     /gene="hda"
                     /locus_tag="PLY05_12840"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HNC04225.1"
                     /translation="MKARHSQMVLNLGPRLDARLSDFAGPSWAAIIAAIHDLLTGKWQ
                     RCFVYGQPDTGKTHLLAAACEHYTEQGHTTLLLSLRELIRTNNPHCLESLEHYDLIAL
                     DDIEMIQGHPDWQEALFHLLNRALEHNTKILLAARAMPTQLGLSLADLVSRLQQAACF
                     GIPSGEDDADREALLLAALGRRNLNLDPEIIRYLLVKGPRYTGLLLKHLSFLEESSLQ
                     QRKKLTLNFVKQLTERF"
     gene            917..>1337
                     /locus_tag="PLY05_12845"
     CDS             917..>1337
                     /locus_tag="PLY05_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC04226.1"
                     /translation="MTNTTKPLHNQDSEYSIDELARVAGTTVRNVRAYQDRGILPPPE
                     RRGRNGIYNHTHLARLRIIGQLLSRGYSIANIGELIVAWEQGQDLSQLLGLESALTSP
                     WSDETPQYYDALELLTLFGASVTQDEIIKAAELGIIEF"
BASE COUNT          404 a          277 c          256 g          400 t
ORIGIN      
        1 gcaattatta tggtactctt acaccatgcc cataatcgtt atcggcaaag tgaattttat
       61 caacgaccaa gcactgatga tgagtctaca tcgacatgaa agcgcgtcat agccaaatgg
      121 tgctcaattt agggccacgt ttagacgctc ggctcagtga ttttgctggc cctagctggg
      181 cagcaatcat tgcggctatt catgatttat taacaggaaa atggcaacgt tgttttgtgt
      241 atggccaacc agacacaggt aaaacccatt tattagccgc tgcttgcgaa cattataccg
      301 aacaaggcca tactacactt ttactttcct tacgcgaatt aattcgcacc aacaaccctc
      361 attgtttaga aagtttagaa cactatgatt taatcgcact ggatgatatt gaaatgattc
      421 aaggtcatcc agattggcaa gaagctttgt ttcatttatt aaatcgagct ttagaacaca
      481 ataccaaaat tttattagcc gcacgcgcca tgcccacaca acttggttta agtttggctg
      541 atttagtgtc gcgcttgcag caagccgcct gttttggtat tcctagtggt gaagatgatg
      601 cagaccgcga agccttatta ttggccgcgt taggcagacg taacttaaat ttagaccccg
      661 aaattattcg ttatttatta gttaaaggac ctcgttatac aggtttatta ctcaaacatt
      721 taagcttttt agaagaaagt tctctacaac aacgcaaaaa actcacttta aactttgtaa
      781 aacaattaac tgaacgcttt tgatctgtac gattgcagga tgacaccaaa ctactgttat
      841 aatagccaat caaccttacc ttttgttatt ggttttcttc aaaagagaag tccatgttat
      901 taagatacgt taaaagatga ccaacactac taagcctctc cataatcaag atagtgaata
      961 ctctattgac gaactcgccc gtgttgcagg aacaacagtt cgcaatgtac gcgcttatca
     1021 agatagaggt attttacctc caccagaacg ccgtggacgt aatggtatct ataatcatac
     1081 ccacttagca cgcttacgaa ttattggtca gttattaagt cgtggctata gcattgccaa
     1141 cattggtgag ctaattgttg cttgggagca aggtcaagat ttaagccaat tattaggttt
     1201 agaatccgct ttaaccagtc cttggtcaga tgaaacaccg caatattatg atgcacttga
     1261 actactcacg ttatttggtg caagtgtgac acaagatgaa attattaagg ctgccgagct
     1321 gggcattatt gaattta
//
LOCUS       DAOQMB010000647         1337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_84556_length_1337_cov_3.979719, whole genome shotgun sequence.
ACCESSION   DAOQMB010000647 DAOQMB010000000
VERSION     DAOQMB010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84556_length_1337_cov_3.979719"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="PLY05_12850"
     CDS             complement(<1..1327)
                     /locus_tag="PLY05_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HNC04227.1"
                     /translation="MMFEWIADPSAWAGLATLVLLEIVLGLDNLVFIAILAEKLPPNQ
                     RNKARTIGLSLALIMRLILLASIAWIVTLTKPLFSIGAFDFSGRDLILIFGGFFLLFK
                     GTLELHERLEGHLAHHSQQKIQHAAFWVVIAQIVVLDAVFSLDSVITAVGMVDHLSVM
                     MIAVVIAVMLMMLASRPLMAFVSRHPNVIVLCLGFLMMIGFSLLVEGLGYHIPKGYLY
                     AAIGFSILVEAFNQAVRRNREQFISSSDLRERTADAVLSLLGGKRSSLSLGETADVIA
                     EKSAEKEVFAPEEKEMIQGVLTLADRPIKSIMTPRPEIDWLNIEDDAETLQAQIKEIG
                     HSRLPIARGTLEQLLGVAATKDILRDLLLEGEISLERSVRQPLIVHESVKVLRVMKQL
                     RESPLQMAIILDEYGSVEGIATPLDILEAIAGEFPDESDEGLSVQQQDDG"
BASE COUNT          440 a          319 c          227 g          351 t
ORIGIN      
        1 aaccatcgtc ttgttgttgc acagaaagcc cttcatcgct ttcatcagga aactcgccag
       61 caatcgcttc taaaatatcc aacggtgtag caatcccctc tactgagcca tattcatcta
      121 agatgattgc catttgtaaa ggtgactcac gaagctgttt catcactcgt agcactttca
      181 cactttcatg aacaattaac ggctgacgga ctgagcgttc taagctgatt tccccctcca
      241 acagtaaatc tcgtaaaata tctttggttg cagcaacccc taataactgt tccaaggttc
      301 ctcgtgcaat tggcaaacgc gaatgaccaa tttctttgat ttgtgcttgt aaggtttccg
      361 catcatcttc aatatttaac caatcaattt ctggtctagg tgtcataatt gatttaatcg
      421 ggcgatccgc taaggttaaa accccctgaa tcatttcttt ttcttcaggg gcaaacacct
      481 ctttttcagc tgacttttca gcaataacat ccgctgtttc gcccaagctt aaactagagc
      541 gtttgccacc taataaactt aacactgcat cggcagtacg ctcacgtaaa tcagaacttg
      601 aaataaactg ttcccgattg cgacgcaccg cttgattaaa ggcttcaact aagatggaaa
      661 agccaattgc cgcatataaa taacctttag gaatatgata gcccaagcct tcaaccagca
      721 aggaaaaacc aatcatcatc aaaaagccta aacacaagac aatcacattg ggatggcgcg
      781 agacaaaagc cattaatggt cgtgaggcaa gcatcatcaa catcacagca atcactaccg
      841 ctatcatcat cactgataaa tgatcaacca tgccaaccgc agtaatcaca ctatccaaag
      901 aaaacacagc atccaatacc acaatttggg caataaccac ccaaaacgcg gcatgttgaa
      961 ttttttgttg ggaatggtgt gctaaatggc cttctaaacg ctcatgcaat tctaaagtgc
     1021 ctttaaataa caagaaaaag ccaccaaaaa ttaaaattaa atcacgtccc gaaaaatcaa
     1081 aagcgccaat actaaacaaa ggtttagtta aggttacaat ccatgcaatt gaggctaata
     1141 atattaagcg catgatgagt gctaaagata agccaatggt tcgtgctttg ttacgctgat
     1201 taggaggtaa tttttcggcc aagatggcaa taaacaccaa attatctaaa cctaagacaa
     1261 tctctaataa gacaagggtt gctaaacctg cccaagctga cgggtctgcg atccattcaa
     1321 acatgatttt ttaccaa
//
LOCUS       DAOQMB010000648         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85158_length_1332_cov_9.430697, whole genome shotgun sequence.
ACCESSION   DAOQMB010000648 DAOQMB010000000
VERSION     DAOQMB010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85158_length_1332_cov_9.430697"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1315)
                     /locus_tag="PLY05_12855"
     CDS             complement(<1..1315)
                     /locus_tag="PLY05_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04228.1"
                     /translation="MAYSVTTQSTTKTLDMVAANTTASDLNVAPIGKTVQDLLDAAAV
                     AAGKTPTTVPNANQTDVYVGTLKVPYYMSTQAAATEANPTPILNTYWKADATKPDFTA
                     SLLGKIPCGAYAAKQVINGITLEPSLSTTTCFPLPLKQSDQIIPVILTVPKGVKPTTG
                     WPISIFQHGITRNRSDMLAIAPSLAKAGFATIAIDLPLHGLASDNPLYKNKLLAGSPA
                     ASLMTENERTFDADFVNNQTGAAGKDDKVDGSGTHFINLASPITSRDNLRQGAADILV
                     LAKSLANLNTDGVAGGDIDTSKVTFTGISLGTIVGTVALGADNLKTIGAASLSVGGGA
                     IPKLLDASKSYGPIIANGLKGNGVLEDTDTYETFMRFAQTLVDSGDPINFAAAAKNNH
                     AIHFTQVLNDLVVPNAALKGDATATQDKVGATGFLSGNTALARAMG"
BASE COUNT          414 a          267 c          231 g          420 t
ORIGIN      
        1 agcccattgc acgtgctaaa gcggtattac ccgataaaaa gcctgttgcg cctactttat
       61 cttgtgttgc agtcgcatca ccttttaaag cagcattagg tactactaaa tcatttaata
      121 cttgagtaaa gtgtatagca tgattatttt tagcagcagc cgcaaagtta attggatcac
      181 cactatctac taaagtttga gcaaaacgca taaaggtttc ataagtatct gtatcttcca
      241 atactccatt tccttttaag ccattggcaa taatcgggcc ataagattta gacgcatcta
      301 ataacttagg aatagcaccg ccaccaaccg ataaagaagc agcacctatt gttttaagat
      361 tatctgcacc caaagcaaca gtacccacaa tagtacctaa agaaatacct gtaaaagtaa
      421 ctttagaagt atcaatatca ccacctgcta cgccatctgt atttaaatta gctaaacttt
      481 tagccaaaac taaaatatca gcggcacctt gtcgtaaatt gtcacgactt gttattgggc
      541 tagctaaatt aataaaatgt gtacctgaac catccacttt atcatcttta cccgctgcac
      601 ctgtttgatt attaacaaaa tcagcatcaa aagtgcgctc attttctgtc atcaaacttg
      661 ctgctggact tccagccaac aatttatttt tatataaggg attatctgat gctaagccat
      721 gcaacggtaa atcaatagca attgtagcaa aaccagcttt tgccaagctt ggtgcaattg
      781 ccaacatatc agaacgattg cgtgttatac catgttgaaa aatactaatt ggccatccag
      841 tagttggttt aacacctttt ggtacagtta atattactgg aataatttga tcactttgtt
      901 taagtggtaa tgggaaacat gttgttgtac taagactagg ttctaatgta atgccattga
      961 ttacttgttt tgcagcataa gcgccacaag gaattttgcc taataaacta gcagtaaaat
     1021 ctggctttgt ggcatcagcc ttccaataag tatttaaaat aggtgtagga ttagcttcag
     1081 ttgcagcggc ttgagttgac atataataag gtactttcaa agttcccaca tatacatctg
     1141 tttgattagc atttggtaca gttgttggcg ttttacctgc cgcaacagca gccgcatcta
     1201 ataagtcttg taccgtttta ccaataggtg ctacatttaa atcgcttgct gttgtattgg
     1261 cagcaaccat atctaaagtt tttgtggtgc tttgagtggt tacactataa gccaacaaaa
     1321 tatcattttc ag
//
LOCUS       DAOQMB010000649         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85204_length_1332_cov_3.358653, whole genome shotgun sequence.
ACCESSION   DAOQMB010000649 DAOQMB010000000
VERSION     DAOQMB010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85204_length_1332_cov_3.358653"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PLY05_12860"
     CDS             <1..476
                     /locus_tag="PLY05_12860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC04229.1"
                     /translation="PVEQLTLESFQISQNVNVTAPFLLIKALLPELEKVKGSIINIAS
                     IHAQLTKPEFVAYATSKSALVGLTQALAVEVGARVRVNAISPAAIATPMLQAGFEGNA
                     AAYTALKSHHPCGDIGVPEEVANLAVFLASNQARFINGANISINGAIGARLHDPV"
     gene            489..>1332
                     /locus_tag="PLY05_12865"
     CDS             489..>1332
                     /locus_tag="PLY05_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04230.1"
                     /translation="MKKWLIQAWNSPTLMSWGSLTARLGGFAITLPLVLRAFTPAELA
                     VWLLCATIASLQIIGDVGFSQTFTRAFAFAYGGAHVEELADARVTKTHRPNATLNMDT
                     LQRIFHTIGPVYLKLSVIAFLVLLCFGSLVLQKPIEALSNHQSDAWLAWAMVLISTTF
                     SLWGNIYTSYLQGSGRIALLRRWEMLSAIAGIISVLIVLLAHGGLLEVVLAQQVWVIV
                     SVARNNWLCKSDANFKVSPFSSVDRIVFASVWPSAWRSGMGVLMSFGIVQLSGIAYTQ
                     VVSTA"
BASE COUNT          326 a          249 c          314 g          443 t
ORIGIN      
        1 cccccgttga acaattaacg ttggaaagtt ttcaaatcag ccaaaacgtg aatgtgacag
       61 cgcctttttt attaataaaa gcattgttac ctgagttgga aaaagtaaag ggcagcatta
      121 tcaatattgc cagtattcat gctcaactaa cgaaacctga atttgtggca tacgcgacca
      181 gcaaatcagc attagttggc ttaacacaag cgcttgctgt cgaagtaggt gctcgtgtac
      241 gtgttaatgc tatttctcct gctgctattg caacaccgat gttacaggct ggtttcgagg
      301 ggaatgctgc tgcttacaca gcccttaaat ctcaccatcc ttgcggtgat attggagtgc
      361 cagaagaagt ggctaaccta gcagtgtttt tagcatcaaa tcaagcgcgg tttatcaatg
      421 gagccaatat aagtataaat ggcgctattg gtgcacgctt acatgaccct gtttaggtgc
      481 gtaccccaat gaagaaatgg cttattcaag catggaactc gccaacactt atgagttggg
      541 gcagtcttac ggcacgtttg ggtggttttg ctattacgtt acccttagtt ctacgtgcct
      601 ttacgcctgc tgagttggcg gtatggttat tgtgtgcaac cattgcatca ttacaaatca
      661 ttggtgatgt tggttttagt caaacattta cacgtgcttt tgcttttgct tatggcggtg
      721 ctcatgttga agagctagct gatgcacgag ttacaaaaac acatcgtcct aatgcaacgc
      781 ttaatatgga tacattacag cgtatttttc acactattgg tcctgtttat ctcaaattgt
      841 cagtaattgc ttttttagtg cttctgtgtt ttggctcttt ggtattacaa aaaccgattg
      901 aggctctatc aaatcatcaa agtgatgctt ggttggcgtg ggcaatggtg ttgatctcta
      961 cgactttttc tttgtggggc aatatataca ctagctattt gcagggtagt ggacgtattg
     1021 ctttgctccg tcgatgggag atgttgtcag caatcgctgg tattattagc gtacttattg
     1081 tacttttagc acatggtggc ttattagagg tggtgttagc tcaacaagta tgggtaatag
     1141 tttcagtagc tcgtaataat tggctttgta aaagcgatgc aaattttaaa gtctcgccgt
     1201 tttcatcggt tgatcgaatt gtttttgcct cggtttggcc tagtgcgtgg cgtagtggta
     1261 tgggtgtgct aatgagtttt ggtattgttc aactttctgg tattgcttat acacaagttg
     1321 tttcgacagc ag
//
LOCUS       DAOQMB010000650         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85272_length_1332_cov_1.758810, whole genome shotgun sequence.
ACCESSION   DAOQMB010000650 DAOQMB010000000
VERSION     DAOQMB010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85272_length_1332_cov_1.758810"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1153
                     /locus_tag="PLY05_12870"
     CDS             113..1153
                     /locus_tag="PLY05_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04231.1"
                     /translation="MNQQALHPKARALLDAQVAYILQKVSGAELVNTLVNEIDLILAN
                     APLLTLNDCGVTPQMIKDTVHHYAIELDLGAGVFDIIGHIARTLHGHDIHQKTTINDI
                     VPDKHIEQILDKILELNTVRDYILNELIINPVYTALASDVMYYGLRDYLLSQLGVGKS
                     QPRFTAVLSWGKTLIERVPPDIEQMVELNLRHYIQKSLSSVLSESQSFLAHIDSQKLR
                     DNLLDIWDDVKHHHISTYQQLLTSVDIEEFIVLSYEFWRDFRQSDYLKMLIDLGIDAF
                     FNRYGEATLAELLEEVGISQKMLVADAFRFAPPVLTVLQQKGLLEPFIRQHLEQFYHS
                     PAVWSILEAKDG"
     gene            complement(1140..>1332)
                     /locus_tag="PLY05_12875"
     CDS             complement(1140..>1332)
                     /locus_tag="PLY05_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04232.1"
                     /translation="CQENALSHKDAKEVVEAYQRAHPDLGTPPITESQTSTSLWLSSM
                     VIFITTACITWIWLMFNRL"
BASE COUNT          409 a          239 c          291 g          393 t
ORIGIN      
        1 gggtgtagaa ccaatgccaa cacataaaaa gtcatagtaa cggggtatta gttgtaaaaa
       61 tactacgctt agcgtaactt ggcaagagac aaacttagga catcagcaag atatgaatca
      121 acaagcactt catcctaaag cgcgggcgtt gttagacgct caagtggcct atattctgca
      181 aaaagtgagt ggtgcagagc tagtcaatac cttggtcaac gaaatagacc ttattttagc
      241 caatgcaccg ttactaactc taaatgattg tggtgttacg ccacaaatga ttaaagatac
      301 agtccaccat tatgccattg aactcgactt gggagcaggt gtttttgata ttattggcca
      361 tattgcgcgt accttacatg gccatgacat ccatcaaaaa acaactatca acgatattgt
      421 gccagataaa catattgagc aaatactcga taaaatatta gagctaaaca cagttcgtga
      481 ctacatttta aatgaactaa taattaatcc agtatatacc gccttggcat cggatgttat
      541 gtattacggt ttgcgtgatt atttgttgag tcaacttgga gttggcaaaa gtcagccacg
      601 ctttacagca gtattgtcat ggggaaaaac gttaatagag cgtgttccac ccgatataga
      661 acagatggtt gagcttaatc tacgtcatta tattcaaaaa agtctcagca gtgtgttgag
      721 tgaaagtcag agttttttag cgcatattga tagtcaaaaa ttacgggaca acttattaga
      781 tatttgggat gacgttaaac accatcacat cagtacttat cagcagctat taacgagtgt
      841 tgatatcgaa gagtttattg tactttcata cgaattttgg cgcgatttta gacaaagtga
      901 ttatttgaag atgctcatag acttgggaat agatgccttt tttaatcgtt atggcgaagc
      961 gactttagca gagttactcg aagaggtagg gattagccaa aagatgcttg tagcagatgc
     1021 tttcagattt gcaccgcctg ttttaactgt attgcagcaa aaaggtttat tggagccgtt
     1081 tattcgacag cacttagagc aattttatca ctcgccagcg gtatggtcga ttttagaagc
     1141 taaagacggt taaacatgag ccatatccat gtgatacagg cagtggtaat aaatatcacc
     1201 atactgctga gccataggct agttgacgtt tgtgactcag tgatgggagg tgtacccaaa
     1261 tcagggtggg cgcgttgata ggcttctact acttcttttg catctttatg gctcaaggcg
     1321 ttttcttggc at
//
LOCUS       DAOQMB010000651         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85285_length_1331_cov_16.485893, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000651 DAOQMB010000000
VERSION     DAOQMB010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85285_length_1331_cov_16.485893"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..>1331)
                     /gene="guaA"
                     /locus_tag="PLY05_12880"
     CDS             complement(154..>1331)
                     /gene="guaA"
                     /locus_tag="PLY05_12880"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HNC04233.1"
                     /translation="GFIDVWMSHGDKVTRLPEGFNITASTPSCPIAAMSCEAKRFYGM
                     QFHPEVTHTLQGEALLRRFVREICACDDLWTPAQIIEDSIRRIREQVGDDHVLLGLSG
                     GVDSSVVAALLHRAIGDKLTCVFVDNGLLRKNEGDMVMDMFAKNMGVKVIRADAEAKF
                     LGELAGVADPEQKRKIIGRVFIEVFDSESAKLNNIKWLAQGTIYPDVIESAASKTGKA
                     HVIKSHHNVGGLPEDMKFKLLEPLRELFKDEVRKIGLELGLPYDMVYRHPFPGPGLGV
                     RILGEVKKEYADVLREADAIFLEELHKAGWYHRTSQSFAVFLPVKSVGVVGDGRRYAW
                     VIALRAVETIDFMTARWAHLPYELIEKVSNRIINEISGVSRVVYDVSSKPPATIEWE"
BASE COUNT          401 a          348 c          238 g          344 t
ORIGIN      
        1 tcgttaattc taataaagcg gcttcagtat tttggatttt ggctgataaa gacatcttcc
       61 ccccccctta aaactcaaaa ggcttgcaat agcaagcctt tacgcttagg acttacgcac
      121 tacataaatc ctaactctat caaaattaga gccttattcc cactcaatcg ttgctggcgg
      181 tttagatgac acatcataga ccacacgcga tacgcctgag atttcgttaa taatacggtt
      241 agacaccttc tcaattaact catacggcaa atgcgcccaa cgtgcagtca taaagtcaat
      301 ggtttcaacg gcacgtaagg caatcaccca tgcataacga cgaccatcgc caaccacacc
      361 gaccgactta actggcaaga ataccgcaaa cgattgactg gtacggtgat accaacctgc
      421 tttgtgcaat tcttctaaga aaatggcatc agcttcgcgc aatacgtcag catattcttt
      481 tttcacttca cccaaaatac gcacgcccaa accagggcca gggaatggat gacgatagac
      541 catgtcatag ggcaagccta actctaaacc aattttacgc acttcatctt taaacaattc
      601 gcgtaaaggt tcgagcaatt tgaatttcat atcttcaggc aagccaccca cattatggtg
      661 tgacttaatg acatgcgctt tacccgtttt gctagcagcc gactcaatga catcaggata
      721 aattgtgcct tgcgctaacc atttgatatt atttaatttt gccgactcgc tatcaaatac
      781 ttcaataaac acacgaccaa taattttacg cttttgttct gggtcagcaa cgcctgctaa
      841 ttcgcccaaa aacttggctt cggcatcggc acgaatgact ttaacgccca tgtttttggc
      901 gaacatgtcc atgaccatat cgccttcgtt tttgcgcaac aaaccattat ccacaaacac
      961 gcaagtgagt ttgtcaccaa tcgcacgatg caataaagcc gctaccacgg aagaatcaac
     1021 accacctgac aagcccaaca acacatggtc gtcacccact tgttcacgaa tacgacggat
     1081 actgtcttca atgatttggg ctggtgtcca caagtcatca caagcacaga tttcacggac
     1141 aaaacgacgt aataacgctt caccttgtaa agtatgggtg acttcaggat gaaattgcat
     1201 accataaaag cgtttagctt cacagctcat agctgcaatt gggcaactgg gtgtgcttgc
     1261 cgtgatatta aaaccttcag gcaaacgtgt gactttgtca ccatgactca tccacacatc
     1321 aataaaacct g
//
LOCUS       DAOQMB010000652         1331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85288_length_1331_cov_10.774295, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000652 DAOQMB010000000
VERSION     DAOQMB010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85288_length_1331_cov_10.774295"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /gene="uvrC"
                     /locus_tag="PLY05_12885"
     CDS             complement(<1..1126)
                     /gene="uvrC"
                     /locus_tag="PLY05_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC04234.1"
                     /translation="MSSAHLQQILANLPHLSGIYKMLGAQGEVLYVGKAKSLKNRVSS
                     YFQKNITHPKTQALVAKICDIELTITHSEVEALLLEQNLIKTLKPPFNILLKDDKSYP
                     YIVVTAKEDFPQISIQRGKKNILQGARIFGPYPSAQSVHEALLLLQRLFQVRQCDDST
                     FKHRDRPCMQYQIKRCRAPCVGLISQPDYQQDVDSTIRFLEGKNTELQQQIGQQMEQA
                     SLALNFEAAAIYRDQLLNLRQIQTQQAVYGEQGEADVFALVIEQGLAAIQVLYVRGGR
                     VLGSKNYIVASTESEPEEIFVEFLSGFYWQQERDIPQEIIINCAVQDEQTQSLKQALF
                     SSYQRKVHIKSRVKEHRAAWLHLAELNAKEAIKSHLQTKMQ"
     gene            complement(1132..>1331)
                     /locus_tag="PLY05_12890"
     CDS             complement(1132..>1331)
                     /locus_tag="PLY05_12890"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HNC04235.1"
                     /translation="LLSPRERQMAEMVVNGQTNQDIADTMGVAPTTLSTYRTRIFSKL
                     EIENDVQLTHLAMRFGILQKK"
BASE COUNT          392 a          264 c          234 g          441 t
ORIGIN      
        1 cttgcatttt ggtttgtaaa tggcttttaa tggcttcttt agcatttaat tctgctaaat
       61 gtaaccatgc tgcacgatgt tctttgacac gcgatttaat gtgtactttt cgttgataac
      121 tactaaacaa cgcttgtttt aatgattgag tttgctcatc ttgtacagca cagttgataa
      181 taatttcttg gggaatatcc cgttcttgtt gccaataaaa accactcaga aactccacaa
      241 aaatttcttc tggctcagac tcagttgagg cgacaatata atttttgctg cctaatacac
      301 gaccaccacg cacatataaa acttgaatgg ctgctaagcc ctgctcaatc accaaagcaa
      361 atacatcagc ttcgccttgt tcaccataaa ctgcttgctg ggtttgaatt tggcgtaagt
      421 taagcaactg atcacgatag atagccgccg cttcaaaatt taaggccaaa gacgcttgtt
      481 ccatctgttg cccaatttgc tgttgtaact cggtattttt tccttctaaa aaacgaatcg
      541 tgctgtctac atcctgctga taatctggtt gactaatcaa tccgacacaa ggtgcacgac
      601 aacgtttaat ttgatattgc atacacggtc gatcacgatg cttaaatgta ctgtcatcac
      661 attgccgcac ctgaaataat cgttgtaata agagtaaagc ttcatgtacc gattgagcac
      721 taggataagg gccaaaaata cgcgcccctt gcaaaatatt ctttttacca cgctgaatac
      781 taatttgtgg aaaatcttct ttggccgtaa caacaatata agggtatgac ttatcgtctt
      841 tgagtaatat attaaatggt ggctttaacg ttttaattaa attttgttca agtaataagg
      901 cttcaacttc actatgggta atagttaatt caatatcaca aattttagca actaaagctt
      961 gagttttggg atgggtgata tttttttgaa aatatgaact gacacgattt tttagggatt
     1021 tagctttgcc aacatataac acctcccctt gtgcacctaa cattttatag atgcctgaaa
     1081 gatgtggcaa attggccaat atctgttgta aatgcgctga agacatttaa attatttctt
     1141 ttgtaagatg ccaaaacgca tggctaagtg cgttaattgc acatcatttt ctatttctaa
     1201 cttagaaaaa atacgtgttc tataagtact caatgttgtt ggtgcaacac ccatcgtatc
     1261 cgcaatatct tgattggttt ggccatttac gaccatttct gccatttgcc tttcacgcgg
     1321 agataacaaa g
//
LOCUS       DAOQMB010000653         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85419_length_1330_cov_15.597647, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000653 DAOQMB010000000
VERSION     DAOQMB010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85419_length_1330_cov_15.597647"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="PLY05_12895"
     CDS             complement(<1..1263)
                     /locus_tag="PLY05_12895"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HNC04236.1"
                     /translation="MLKRLTHQALKLACARADRNFRGQGKQLEQVQRQKLQHMLQQVA
                     SVQNKARSLIPTWEEYAHQQPVTRYAQWQEQIHAQRRGESRLTSSPLVRYQPTSGSSE
                     KLKLIPYTKTFIDQLDAAIAPWLASMYRQYPKMANGTHYWSVSWLPQSQFDDLSGNLN
                     DDSELLGVVKRVLAAQSQAVPADVALAASADDALFATLCYLVADRQLRLLSVWSPTFA
                     LQLLDALPVWADGIIDVLQNGNWRGRQLSLKQLTAPYAPSRAQELKRILALPKAVWVA
                     ELWPDLALVSAWDTADAAPWAEQLQALIPHAAFEGKGLWATEGVVTIPIDGHYPLAYQ
                     SHFYEFEDLTNGGILASWQLKEGDVVSPIITSGNGLLRYQLDDRIVVSEFWQGVPCFK
                     FLGRRFGVDMVGEKMSPDAARQALATVAQ"
BASE COUNT          407 a          316 c          263 g          344 t
ORIGIN      
        1 ttgggcaacc gttgctaaag cttgacgtgc ggcatcaggt gacatttttt caccaaccat
       61 atccacacca aaacgacgac ctaaaaattt aaaacaaggg acaccctgcc aaaattcact
      121 gacaacaata cgatcatcta attgatagcg taataagcca ttaccactgg taataattgg
      181 gctaaccaca tcgccttctt tcaactgcca actggccaaa attccgccat tggttaaatc
      241 ttcaaactca taaaaatgac tttgataggc taatggataa tggccatcta tagggatagt
      301 gaccactcct tcggtcgccc ataaaccctt accctcaaac gcagcatgag gaattaaggc
      361 ctgtaattgt tcagcccaag gcgctgcatc agcagtatcc caagccgaaa ccaacgctaa
      421 atctggccac aattctgcaa cccagactgc tttaggtaag gctaaaatgc gttttaattc
      481 ttgtgcacga ctcggtgcat atggggcagt taattgtttt aacgataatt gacgaccacg
      541 ccaattacca ttttgcaaaa cgtcaataat gccatctgcc caaacaggta atgcatccaa
      601 taattgcaaa gcaaacgttg gactccaaac agacaataaa cgtaattgac gatcagcaac
      661 caaataacac aaggtagcaa ataaggcatc atccgcactt gcagccaaag cgacatcggc
      721 aggcacagct tgtgattgag cggctaatac acgtttaaca acgcccaaca actcactatc
      781 atcatttaaa ttaccactta aatcgtcaaa ttgactttgt ggcaaccaag ataccgacca
      841 ataatgagtc ccattagcca ttttgggata ttggcgatac atactggcta accaaggagc
      901 aatggcagca tctaattggt caataaatgt tttggtataa ggaattagct ttagtttttc
      961 ggatgaacca ctggttggct gataacgcac taaagggcta ctggttaaac gactctcacc
     1021 acgacgttgg gcatggattt gctcttgcca ttgtgcataa cgtgtgactg gctgctgatg
     1081 agcatactct tcccatgttg gaattaaact acgtgcttta ttttgaacac tggccacttg
     1141 ttgcagcata tgctgcaatt tttgacgttg gacttgttcg agttgtttgc cttgaccacg
     1201 aaaattgcga tcagcacgcg cacaagctaa ctttaaggct tgatgagtaa gtcgttttaa
     1261 catcgttaac cccttatgcg cgacgttgca aaggagtcac ttcggctaag gtgcgatgca
     1321 aaggctcaac
//
LOCUS       DAOQMB010000654         1330 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85422_length_1330_cov_7.681569, whole genome shotgun sequence.
ACCESSION   DAOQMB010000654 DAOQMB010000000
VERSION     DAOQMB010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85422_length_1330_cov_7.681569"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..620)
                     /locus_tag="PLY05_12900"
                     /pseudo
     CDS             complement(186..620)
                     /locus_tag="PLY05_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004680746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            complement(633..>1330)
                     /locus_tag="PLY05_12905"
     CDS             complement(633..>1330)
                     /locus_tag="PLY05_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04237.1"
                     /translation="SIYPHYKKEPVVNQLLLETLVKLQAWQDCLEWLPIVAKAPVAET
                     VTNQALMKTAYQHSLAMLAKTTGRIDKTITFRQLQEFWQDIPRQHQRDADVVMSYVQA
                     LISVGFYDEASVIIARVLNENWHHGLAELYGRVLHAEPELALEQAKTWLKLHPQSPVL
                     LLTLGRLSMQCRDWTMAKAYFEQSLVLHKNTETYAEFVRLLQHLNDPEAGHYLIAGLN
                     QLINQPLPNLPLP"
BASE COUNT          392 a          265 c          228 g          445 t
ORIGIN      
        1 agattgcaca ctaaattagc aagcttttgt tactatttaa tgtacattta acagggattt
       61 tttggtggtt tatttgttaa caatgacttt attgctcttt atttatctcg taaatgattt
      121 aattttgaca aggctttgcc taataacgtt tctggttgtt tatgcggatt taaggcatct
      181 acttctaaat cattacctac tgtttgctct aaattaataa tcgtgtgtct aaactcttcg
      241 ggtaaagtga ctttttggcc atttttatca accatcacca caattgcttc acctgtggca
      301 acaatcgttt tgtgttgttg actaaaaatc gcgtattcca ttctaaaacc tgatgcacgt
      361 aattcaacca cacgcgcacc taattctaat tcatcaggat aattaaccga ttttaaataa
      421 cgacaactat tagcagcaac tacgggattc atttgtacca aatgttctaa agcaccaatt
      481 gcttgtaaat aactaatgcg tacactttca aaataacggt aataaacgac gttattaaca
      541 tgaccaaaag cgtccatttc accccatgcg actgtttgtg cctgcacaat aggaaattcg
      601 tttaaactga tttggctcat tatcattctc aactagggca aaggtaaatt aggtaagggt
      661 tgattaatta attgatttaa acccgcaatt aaataatgac ctgcttcggg atcattgaga
      721 tgttgtaata aacgcacaaa ttcagcatag gtttcggtgt ttttatgcaa cactaaactt
      781 tgctcaaaat aggctttggc cattgtccaa tctcggcact gcatacttaa acgccctaaa
      841 gttaataaca aaactggact ttgtggatgg agtttcaacc atgttttagc ttgttctaag
      901 gctaactctg gttctgcatg aagcactcgc ccatacaatt cagccaaacc atgatgccaa
      961 ttctcattta aaacacgggc aataataaca cttgcctcat catagaaacc gacacttatc
     1021 agagcttgta cataactcat taccacatcg gcatcacgtt gatgttgacg tggaatgtct
     1081 tgccaaaatt cttgtagttg cctaaaggta atcgttttat caattcgacc cgttgttttg
     1141 gctaacatgg ctaagctgtg ttgataggct gttttcatta atgcctgatt ggtcacggtt
     1201 tcggcaacag gtgctttggc gactatgggc agccattcta aacagtcctg ccatgcttgt
     1261 aacttaacca atgtttctaa taataattga ttaacaaccg gctctttttt gtaatgtgga
     1321 taaatgcttt
//
LOCUS       DAOQMB010000655         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85518_length_1329_cov_19.545526, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000655 DAOQMB010000000
VERSION     DAOQMB010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85518_length_1329_cov_19.545526"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /gene="bioA"
                     /locus_tag="PLY05_12910"
     CDS             complement(<1..1295)
                     /gene="bioA"
                     /locus_tag="PLY05_12910"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HNC04238.1"
                     /translation="MTNQQLMQRDLAVLWHPCTQMHDHEHIPLVPIAKAQGIWLEDFE
                     GKRYIDAISSWWVNIWGHAHPHINQAIKQQLDTLEHVILAGFSHEPVIELAEKLVALA
                     PQGLSRCFFADNGSAAVEVALKMSMHYWHNQGETQKTRFVSLQGSYHGETLGALAVTD
                     IPLFSETYAPLLTQHYRVPSPDWTQAPEGMTAEAFARQQFNALEQLLIEKHSEICAVI
                     VEPLIQGAAGMKMYHPVYLQLLRAACDKYGVHLIADEIAVGFARTGRFFACEHANIRP
                     DFLCLSKALTAGYLPMSVVLTTEAIYQGFYHPYSTLKGFLHSHSFTGNPLAARAALAS
                     LELFALDPQLQRQQQIHHVCQKALEPLKNHAHIVNIRQTGTICAFDLVQDKASKTPFD
                     WRERRGLQVFQAALEQRVLLRPIGNTVYVMPPYVMTDDE"
BASE COUNT          384 a          284 c          297 g          364 t
ORIGIN      
        1 aattcatcat cggtcattac atacggcggc atcacataca cggtattacc aatagggcgt
       61 aataataccc tttgttctaa cgcggcttga aacacctgca aaccacgacg ctcacgccaa
      121 tcaaacgggg ttttgcttgc tttgtcttgc accaaatcaa acgcgcaaat cgtgcctgtt
      181 tgtcgaatat tgacaatatg agcatgattt tttaacggct ctaaggcttt ttggcaaacg
      241 tgatgaattt gctgttggcg ttgtaattgt gggtctaagg caaataattc taaagaagcc
      301 aacgcggctc ttgcggctaa aggatttccg gtaaaactat gtgaatgtaa aaaacctttg
      361 agtgtagagt aagggtgata aaatccttga taaatagctt cagtggttaa gactaccgac
      421 atgggtaaat aacctgcggt taaggcttta gataaacaca aaaaatcagg acgaatattg
      481 gcgtgctcac aagcaaaaaa acgacctgta cgggcaaaac caacggcaat ttcatcagca
      541 attaaatgca caccatattt gtcacaagcg gcgcgtaata actgcaaata aacaggatga
      601 tacattttca tgcctgctgc gccttgaatc agtggctcaa caatcaccgc acaaatttcg
      661 ctgtgctttt caattaacag ttgttcaagg gcattaaatt gttggcgggc aaaggcttcg
      721 gctgtcatgc cttcgggggc ttgtgtccaa tctggcgatg gcacacgata atgttgggtg
      781 agtagtgggg cgtaggtttc cgaaaacaac ggaatatcag tcacggctaa tgcgccaagc
      841 gtttcaccat gatagctacc ttgcaaggac acaaaccgtg ttttttgcgt ttcaccttga
      901 ttatgccaat agtgcatact catttttaag gcaacttcaa ctgctgccga gccattatca
      961 gcaaaaaaac aacggcttaa accttgtggt gcaagggcaa ctaatttttc ggccaactca
     1021 atcacgggtt cgtggctaaa acccgccaag atcacatgct caagggtatc taattgctgt
     1081 ttgatggctt gattaatatg tgggtgggcg tgtccccaaa tattgaccca ccacgagcta
     1141 atggcatcaa tatagcgttt accctcaaag tcttctaacc aaataccttg cgctttggca
     1201 attgggacta aaggaatgtg ttcgtggtcg tgcatttgtg tacaagggtg ccacaagact
     1261 gctaaatcac gttgcattag ttgttggtta gtcattgttt ttgcctcgta aaatcaacga
     1321 ggcattata
//
LOCUS       DAOQMB010000656         1327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85783_length_1327_cov_11.774371, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000656 DAOQMB010000000
VERSION     DAOQMB010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85783_length_1327_cov_11.774371"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..361)
                     /gene="nqrM"
                     /locus_tag="PLY05_12915"
     CDS             complement(140..361)
                     /gene="nqrM"
                     /locus_tag="PLY05_12915"
                     /inference="COORDINATES: protein motif:HMM:NF016299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HNC04239.1"
                     /translation="MSLFFVCLVAIVLMMLLMAVGVLMGKEPLKGTCGGLNRFTGDKE
                     CPVCGGDTQKCETSKQTNNPLIKDAMRDD"
     gene            complement(358..>1327)
                     /locus_tag="PLY05_12920"
     CDS             complement(358..>1327)
                     /locus_tag="PLY05_12920"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNC04240.1"
                     /translation="AKEPVSFSGVAYYSMPWAVKIDALPAHVSVQQLQQELQASLDYA
                     NKVLSTYQNDTELMQFNQSPMGQWQQLSPMLFNTIQTSLTVSDATQGVYDVTVGALVE
                     LWGFGKQAVPSHIPSPIEIDTARQRVGWQSVLLNPQTNQAQRQKDVFLNLSSLGEGVG
                     VDELEKVLAKHQIHSYMLSVAGTLKTQGLKLNGQTWQIAVEKPDGSGLPERVLNLGQQ
                     QAVVSTSGSYRNYHEIDGVRYSHTIDPRTAKPISHNTVSTTVVFRQQQAAYADAWATA
                     LNVLGAKEGLALAEKQKIAVYYLLKTPTGFAEQYSSTFKVYLEQQP"
BASE COUNT          385 a          308 c          241 g          393 t
ORIGIN      
        1 gctaaaaagt acaccgaagc tgccatgccc aatacacaaa acacggaagg ccataattta
       61 ctaaagcctt cggtgtattt catggttact gcccattgca cttctaataa gccagcaatg
      121 attaaataaa gccaagccat tagtcgtctc tcatggcatc tttaattaaa ggattattcg
      181 tttgtttact agtttcgcat ttttgggtat caccaccaca aacaggacac tctttatcgc
      241 ctgtaaaacg atttaagcca ccacacgtgc cttttaacgg ctctttaccc atcaatacac
      301 cgaccgccat taataacatc attaacacaa tcgcgactaa acacacgaaa aataagctca
      361 tggctgttgc tccaaataca ctttaaacgt cgaactatat tgctcagcaa aacctgttgg
      421 cgtttttaat aaataataga cggcaatttt ttgtttttct gcaagtgcta aaccttcttt
      481 tgcccccaat acattcaggg ctgttgccca tgcatcggca taggctgcct gttgttgacg
      541 aaaaacgaca gtcgttgata ccgtattatg actaattggt ttagcagtgc gtggatcaat
      601 ggtatgtgaa taacgaacac cgtctatctc atgataatta cgatatgagc ccgatgtcga
      661 gacgacggct tgttgttgac ctaaatttaa tacacgttca ggcaaaccac tgccatcagg
      721 tttttctacg gcaatttgcc atgtttgacc atttagtttt aaaccttggg tttttaatgt
      781 gcccgccact gataacatat aactgtgtat ctgatgtttg gctaagactt tctctagttc
      841 atcaacgcca acaccctcac ccaacgagga tagatttaaa aagacatctt tttgacgttg
      901 agcttgatta gtttgtggat ttaataatac cgattgccaa cccacacgct gccgcgcagt
      961 atcaatttcg attgggctag gaatatgact tggcacagct tgtttaccaa aaccccataa
     1021 ctccactaat gcccccactg tcacatcata aacgccctga gtagcatcac tcacggttaa
     1081 cgaagtctga atagtattga ataacatcgg actaagttgt tgccattgcc ccataggact
     1141 ttggttaaat tgcattaatt cggtatcgtt ttgataggtc gacaatactt tattagcata
     1201 atctaaacta gcttgtagtt cttgttgcag ttgttgcacg ctgacgtgtg cgggcaaagc
     1261 atcaattttt accgcccaag gcatactata ataagcaacg cccgaaaaac tgacaggctc
     1321 tttggca
//
LOCUS       DAOQMB010000657         1326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_85914_length_1326_cov_10.672699, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000657 DAOQMB010000000
VERSION     DAOQMB010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85914_length_1326_cov_10.672699"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..>1326)
                     /locus_tag="PLY05_12925"
     CDS             complement(422..>1326)
                     /locus_tag="PLY05_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HNC04241.1"
                     /translation="AALAIDFVGLNKNGQQTVSFKSALKWSLVWFSLALAFGALLWWE
                     TTNTFDSAVATEKAAEYFTGYLVEKSLAVDNVFVFLMIFSYFALPIHLQRRVLLYGIL
                     GAIILRAIMIFVGGWLLTEFHWLMYVFGAFLVLMGIKMWQGADEDASLDDNKLLNWLK
                     NHIPLSKDYDGEKFFTWENGKRVATPLFLVLIFVELSDVIFAVDSIPAIFAITSDPFI
                     VLTSNIYAILGLRAMYFMLAGVADKFSLLNYGLAIVLVFIGTKMLLLDVFKIPALVSL
                     AVIIAILSVTMWLSLHKAKKGMKA"
BASE COUNT          489 a          293 c          180 g          364 t
ORIGIN      
        1 tttggcgtaa tcgatcattt ttaaccaacg tccattgatt ggttaatttc atttgcaatt
       61 catcaatatc atcattagaa gcataagcag gcatataagt cacagccatc agtgtgctaa
      121 tggcaacaat ggcttttaat ggtcgaatca cgtctgatct ccgttattat tatgcgtttg
      181 gtgtgagtgg tcttattgtt atcgcctaaa ccatccctca aacgacaatt atcattttat
      241 gatgacatat catacaaaaa atcttgtatc agaaaatgac taaaaacgcc aaaaaattaa
      301 ccaatcctcg atattaaaaa tttctcagta agatggttaa caggcaaaat ctccacagtt
      361 ccgatagttt taataaaaaa ggcttggttt cccaagcctt tttattaatc agattttgcc
      421 attaagcttt cattcctttt ttagctttat gcaaactcag ccacatagtt acgctgagga
      481 tagcaataat cactgccaaa gacactaagg caggaatctt aaacacatcc agcaacaaca
      541 tcttagtacc aataaacact aagacaatcg ctaaaccata atttaacaag ctaaatttat
      601 cagctacacc tgccaacata aaatacatcg cacgtaagcc caaaatcgca taaatatttg
      661 atgttaaaac aataaatggg tcgctagtaa tcgcaaaaat agcaggaata ctatccactg
      721 caaaaatcac atcacttagc tcaacaaaaa tcaaaaccaa gaataacggc gttgccacac
      781 gtttgccatt ttcccaagta aaaaactttt caccatcgta gtctttagac aaaggaatat
      841 gattttttaa ccaattcaat agcttattgt catctaaaga tgcatcttca tctgcacctt
      901 gccacatttt aatacccatc agtactaaaa atgcgccaaa aacatacatc aaccaatgga
      961 actctgttaa caaccaaccg ccaacaaaaa tcatgattgc acgtaaaata atcgccccta
     1021 aaatgccata taacaatacg cgacgttgca aatgaattgg gagcgcaaaa tagctaaaaa
     1081 tcattaaaaa cacaaaaacg ttatcaaccg ctaaagactt ttcaaccaaa taacctgtaa
     1141 agtattcagc ggctttttcg gttgcaacag cactatcaaa ggtatttgtt gtttcccacc
     1201 acaataaagc accaaaggct aacgctaaac taaaccatac taacgaccac ttaagcgcac
     1261 ttttaaaact cactgtttgc tggccatttt tatttaagcc aacaaaatca atggcaagtg
     1321 cagcaa
//
LOCUS       DAOQMB010000658         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_86052_length_1325_cov_6.729921, whole genome shotgun sequence.
ACCESSION   DAOQMB010000658 DAOQMB010000000
VERSION     DAOQMB010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86052_length_1325_cov_6.729921"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..434)
                     /locus_tag="PLY05_12930"
     CDS             complement(48..434)
                     /locus_tag="PLY05_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC04242.1"
                     /translation="MNRHKNLLLDTHTLLWVLNDAPQLSKAKPYIEQANNIYVSMASW
                     WEISIKASLNKLPIDPQMIFIEAQQAGIKMLPIQIAHCQKLLSLATIHRDPFDRMLIA
                     QAISEEMPLLTHDQTLASYSSLVILF"
     gene            complement(431..697)
                     /locus_tag="PLY05_12935"
     CDS             complement(431..697)
                     /locus_tag="PLY05_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04243.1"
                     /translation="MITLPVTPSSSIELSALLIDQIEHGEIVTLTKNGQPIAQILPFK
                     PLENQQGRQLGSIRIGTMKGLLNIPDDFDTPLPDDLLDAFEGKE"
     gene            complement(763..>1325)
                     /locus_tag="PLY05_12940"
     CDS             complement(763..>1325)
                     /locus_tag="PLY05_12940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994281.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNC04244.1"
                     /translation="SNICGVAQSIEPTYQNLYVKSNLSGEFTVVNPYLVNALKERGLW
                     DPVMVNDLKYYDGSVQNIERIPEDLKALFATAFEVEPRWMVESASRRQKWIDQAQSLN
                     LYISHASGKKLDVTYRMAWYRGLKTTYYLRAIGATAAEKSTISDGALNAVKAQVQVVH
                     EESAFVEAAPVPLACSIDNPDCEACQ"
BASE COUNT          402 a          256 c          259 g          408 t
ORIGIN      
        1 ttttcttcgg cataaaaatc atcccaattt aacatggtgg ttctctctta aaataaaata
       61 acaagtgaac tgtaagaagc taatgtttgg tcatgtgtta ataatggcat ttcttcgctg
      121 attgcttgag cgattaacat tcggtcaaat gggtcacgat gaatagtggc caaactgagt
      181 agtttttgac aatgcgctat ttgaataggg agcattttta tacctgcttg ttgtgcttct
      241 ataaaaatca tttgagggtc tattggcagt ttatttaacg atgccttaat tgaaatttcc
      301 caccaagatg ccatgctaac gtagatgttg tttgcttgtt caatataagg cttcgcttta
      361 gataattgag gagcatcatt caatacccaa agtaaagtat gtgtatctaa caacaaattt
      421 ttgtgtctat tcattctttg ccttcaaatg cgtctaataa atcgtcaggc aaaggagtat
      481 caaaatcatc ggggatattt aataaacctt tcattgtgcc aatacgaata gagccaagct
      541 gtcttccctg ttgattttca aggggcttaa aaggcaaaat ttgggcaata ggttgaccat
      601 tcttggttag agtgacgatt tcaccgtgct caatttggtc tatcaataat gcagataatt
      661 caatcgagct agagggggta acaggtagag taatcatgtg atcctcgcat acaacggata
      721 agactaagat ggtatttaaa acatcttagt ctctataacg gcttactgac aagcttcaca
      781 atcaggatta tcaatactac aagctaatgg cacaggtgca gcttctacaa aagcagattc
      841 ttcatgtacg acttgtactt gtgctttaac cgcatttaat gcgccatcac taatggtcga
      901 tttttcagca gccgttgcgc caattgcacg gaggtaatag gtggttttta agccacgata
      961 ccaagccata cgataagtca catccaattt tttgcccgac gcatggctga tgtacaagtt
     1021 taaggattgc gcttggtcaa tccatttttg acgacggcta gcagactcaa ccatccaacg
     1081 cggttcgact tcaaaagcag tggcaaacaa agcttttaaa tcttctggaa tacgttcgat
     1141 gttttgtacc gaaccatcat agtattttaa gtcattaacc atgacagggt cccacaaacc
     1201 gcgctctttt aacgcattga ctaaataagg gttaaccacg gtaaattcgc ccgataagtt
     1261 cgacttaaca tacaagtttt gataagttgg ctcaatagat tgtgccacac cacaaatatt
     1321 agaaa
//
LOCUS       DAOQMB010000659         1325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_86079_length_1325_cov_3.548819, whole genome shotgun sequence.
ACCESSION   DAOQMB010000659 DAOQMB010000000
VERSION     DAOQMB010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86079_length_1325_cov_3.548819"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLY05_12945"
     CDS             <1..700
                     /locus_tag="PLY05_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNC04245.1"
                     /translation="GKPFFTDNPVTRISHTRAWTPEKRRTTIIRANELPLWIKAVDEL
                     RASDNNCIDESPALVADYLEFCLFSGLRRDDVLCLRWEQVELDTALMHPVIHKKQKEV
                     ITLPLTDYLIAILERRKKVQVNEYVFAGRGGDGRFDDPKRQIAKVVKLTGIHFSSHDL
                     RRTFISVAESLDISTYAIKRLCTHSLGGDVTAGYIQMDVERLRAPMQKITDYFLKAMG
                     KNATANIVTLLKQG"
     gene            713..970
                     /locus_tag="PLY05_12950"
     CDS             713..970
                     /locus_tag="PLY05_12950"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNC04246.1"
                     /translation="MPEISRFFGIIITMYWRDHNPPHFHASYGNDEALITMNGEVYSG
                     SLPRRALSLVREWLAIHRQELLDNWENAQKREPMKSIEPLE"
     gene            975..1241
                     /locus_tag="PLY05_12955"
     CDS             975..1241
                     /locus_tag="PLY05_12955"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNC04247.1"
                     /translation="MILHVTNTKVLPNYCLWLQFDDGTEGYADLSGELWGAMFEPLKD
                     PVVFASAKVDPVCKTVTWSNGADLAPEYLKQLLVEQTNQHQKMA"
BASE COUNT          395 a          252 c          296 g          382 t
ORIGIN      
        1 cggcaaacct tttttcactg ataatccagt cacccgtatt tctcatacaa gagcttggac
       61 acccgaaaaa cgccgcacaa ccattattag agccaatgag ctaccgctat ggataaaagc
      121 tgttgacgag ctaagagcca gtgacaataa ctgtatagat gaatcgccag cattggttgc
      181 tgattattta gagttttgtc tttttagtgg tttacgccgt gatgatgttt tgtgcctgcg
      241 ttgggagcaa gtagaacttg ataccgcctt aatgcacccc gttatccata aaaaacaaaa
      301 agaagtaata actttgcctt taactgatta tttaatagct attcttgaaa ggcgtaaaaa
      361 ggtacaagta aatgaatatg tttttgctgg ccgtggtggt gatggccgtt ttgatgaccc
      421 taaaagacaa attgccaaag tggtgaaact cactggtatt cacttctcct ctcacgattt
      481 aagacgtaca tttatatcag tagctgaaag tttggatata tcaacctatg caattaaacg
      541 cttgtgtacg cacagcttag gtggcgatgt gaccgcaggt tatattcaaa tggacgtaga
      601 gcggttacgc gcccctatgc agaagattac agattatttt ttaaaagcaa tgggcaaaaa
      661 cgccactgcc aatattgtca ctttattaaa acaagggtaa ggagttttaa caatgcccga
      721 aattagccga ttttttggaa tcattattac aatgtactgg cgtgaccata atccgcctca
      781 ttttcatgcg agctatggca atgatgaggc gttaatcacc atgaatggag aggtatatag
      841 tggtagcttg ccgcgtagag ccttgtcatt ggtgcgtgaa tggttagcga ttcaccgtca
      901 agagttactg gataattggg aaaatgccca aaaaagagag ccaatgaagt cgattgagcc
      961 gttggagtaa tactatgatt ttacacgtta ccaatactaa ggttttacct aattattgtt
     1021 tgtggttgca gtttgatgat ggtacggagg gttatgccga tttatcgggg gaactatggg
     1081 gggcaatgtt tgagccactc aaagaccctg ttgtatttgc ctccgctaaa gttgaccctg
     1141 tgtgtaaaac ggtcacttgg tcaaatggtg cagatttagc acccgaatac ttaaaacagt
     1201 tattagttga acaaacaaac caacatcaaa aaatggctta acctcattaa ccacacctag
     1261 ccagtcgcga ggcgaaagaa cggcaaccct taaccgtttt ggtgtggtta tctttttaag
     1321 ggata
//
LOCUS       DAOQMB010000660         1324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_86175_length_1324_cov_12.970843, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000660 DAOQMB010000000
VERSION     DAOQMB010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1324
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86175_length_1324_cov_12.970843"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="PLY05_12960"
     CDS             complement(<1..245)
                     /locus_tag="PLY05_12960"
                     /inference="COORDINATES: protein motif:HMM:NF021049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HNC04248.1"
                     /translation="MEQQAALSFLQQYLQEHLPLTVAMQVQPQIYDGQSLQLFAPLAV
                     NHNDKGTAFAGSLSSLATVCGWCLVMLWTKEKYQQAC"
     gene            complement(249..809)
                     /locus_tag="PLY05_12965"
     CDS             complement(249..809)
                     /locus_tag="PLY05_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004971488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HNC04249.1"
                     /translation="MDTLTALLTRTSHGALTEPAPNSAVLNQAFQAALRAPDHRMLRP
                     WRYLIIDGEARQRLGESFLQAGLQDNAQLADVEQQRLLKMPLRAPMIIVAITSFKEDV
                     KVPMIEQTLSTGAAVQNFLLALHAQGFASMWRTGWVTEHPIIKQALAIQNNEQIAGFV
                     YVGTAATSLKTPTLLNVDDFVQTWQG"
     gene            complement(905..>1324)
                     /gene="recR"
                     /locus_tag="PLY05_12970"
     CDS             complement(905..>1324)
                     /gene="recR"
                     /locus_tag="PLY05_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNC04250.1"
                     /translation="CRTLTEQKICQICANHARDHHVLCVVESPADILAIEQSGSYSGL
                     FFVLAGHLSPLDGIGPQELGIPLLINRIKQEGIEELVLATNATVEGEATAHYIREACK
                     HYAVKVTRIAHGVPLGGELEMVDSHTLARALSQRQSY"
BASE COUNT          393 a          281 c          253 g          397 t
ORIGIN      
        1 tgacaagctt gttgatattt ttcttttgtc catagcatca ccaaacacca accacatacc
       61 gtggctaagc tacttaaact accagcaaag gctgtgcctt tgtcgttatg attgaccgct
      121 aatggtgcaa aaagttgtaa agattggcca tcataaattt gaggctgtac ttgcatcgca
      181 acagttaagg gtaaatgctc ttgtaaatac tgctgcaaaa aactgagtgc ggcttgctgc
      241 tccatgattt aaccctgcca tgtttgcaca aaatcatcaa catttaacaa agttggggtt
      301 tttaatgaag ttgctgctgt gcctacatag acaaaacctg caatttgttc attattttga
      361 atagctaaag cctgtttaat aattggatgt tctgtgaccc aacctgtacg ccacatacta
      421 gcaaaacctt gagcatgtaa agctaataag aagttttgta ccgccgcacc tgtacttaag
      481 gtttgttcaa tcatcgggac tttgacatct tctttaaagc tagtaatggc tacgataatc
      541 attggtgcgc gtaaaggcat ttttaataat cgttgttgtt caacatcggc taattgagca
      601 ttatcttgta aacctgcttg taaaaaagac tcgcccaaac gctgacgtgc ctcgccgtca
      661 ataatcaaat atcgccacgg acgcaacatt cggtggtcgg gcgcacgtaa tgcggcttga
      721 aaggcttgat ttaaaacggc tgaattggga gctggctcag ttaatgcacc atgagaagta
      781 cgagtcaata aagcagttaa agtatccatt aagtttatct cttgccaaat aatgctaaat
      841 tttctgcaac ttagccttta aagtggcttt atgacaagga ttttttagtt acaatcaaaa
      901 ctatttaata actctgtctt tgactcaaag ctcttgccaa ggtgtgacta tcaaccattt
      961 ctaattcgcc acctaagggc acaccatgag caatacgcgt tacttttacc gcgtaatgtt
     1021 tacaagcttc acgaatataa tgagcggtgg cttcgccttc gacagtggca ttggttgcta
     1081 ataccaactc ttctatgcct tcttgcttga tacgattgat taataatgga atgcctaatt
     1141 cttgcgggcc tataccatct aaaggtgata aatgtcctgc taatacaaaa aacaaaccac
     1201 tataactgcc gctttgctca atcgctaaaa tatcggcagg tgactcaacc acacacaaca
     1261 catgatgatc gcgggcatga ttggcacaaa tttgacaaat tttttgttcg gttaaagttc
     1321 gaca
//
LOCUS       DAOQMB010000661         1323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_86427_length_1323_cov_1.614353, whole genome shotgun sequence.
ACCESSION   DAOQMB010000661 DAOQMB010000000
VERSION     DAOQMB010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86427_length_1323_cov_1.614353"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="PLY05_12975"
     CDS             <1..1032
                     /locus_tag="PLY05_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04251.1"
                     /translation="LYSDDALRGALTQALTYGNQADRTNALLRLTPLVKNPEQLTDAI
                     GKSFRQLYADNVMNGVPNAAENLNTLSAAEGGKLYSPVGSTGLTLNQGSGLQTTGNPV
                     LFGKTTAKLDSEINKNKAQARQADSTVKLNNWRQNQPQKTIGYDANGNMVLVDKRTGQ
                     SMPVGGGISNKPPVKLPPTALKMQQELVDTIGTLGATNTRLSNIVGQLESGKLDLGPW
                     SRLAGSIRNNTNNSNENSRNLSALQATLEELRNASLRINAGVQTDGDAQRAWSEIAAN
                     PNDTELVKSRLKFIMQKNQEAAQLKKLQIEQLRQNFNQPQLQNYGDGGMGGQGGGQDQ
                     VFDFNDLPD"
     gene            1041..>1323
                     /locus_tag="PLY05_12980"
     CDS             1041..>1323
                     /locus_tag="PLY05_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04252.1"
                     /translation="MPDVRMPDGTIIKNVPQGTTKAQLMAKLQKKQQQQQVEKPYSDG
                     KVALSSVVKGLASIPDAVLNTPANLMNLGKAATGYVMNESGYGDLAQRLG"
BASE COUNT          427 a          263 c          306 g          327 t
ORIGIN      
        1 ctttatagtg atgatgcgtt aagaggcgcg ttaactcaag ccttaacata tggcaatcag
       61 gcggatagaa caaacgcatt gcttcgctta acgccattgg ttaaaaatcc tgagcaatta
      121 actgatgcga ttggtaaatc gtttagacag ctttatgctg ataatgtgat gaatggtgta
      181 cctaatgcgg ctgagaattt gaacacgtta agcgcagcag agggcggcaa attatatagc
      241 cctgtaggca gcactggttt aactttaaat caaggaagtg gtttgcagac tacgggcaat
      301 cctgttttgt ttggcaagac cactgcaaaa cttgattcag aaataaataa aaataaagcg
      361 caggcaagac aggcggattc gacagtaaaa ctaaacaatt ggcgacaaaa tcagccacaa
      421 aaaacgattg gctatgatgc caacggtaat atggtgcttg ttgataagcg tacaggtcaa
      481 tctatgcctg tgggcggtgg tattagtaat aagcctcctg taaaactgcc accaactgca
      541 ctgaaaatgc agcaagagct agttgatacc attggcacac ttggcgcaac aaatacgcgc
      601 ctaagcaata tcgttggcca gcttgagtca ggaaaacttg atttaggccc ctggtctcgc
      661 ttagctggct ctattcgcaa taacaccaat aattcaaacg aaaacagccg taatttatcg
      721 gcattgcaag cgacacttga agaattaaga aacgcatcat tgcgaataaa tgcaggggtg
      781 caaaccgatg gtgatgctca acgcgcttgg agtgagattg ctgccaatcc taatgacact
      841 gagcttgtaa aaagccgact aaaatttatt atgcagaaaa accaagaggc cgcgcagctt
      901 aaaaaactac aaattgagca gcttcgccaa aacttcaacc agccacaatt gcaaaactat
      961 ggtgatggcg gcatgggtgg tcaaggcggt ggccaagacc aagtttttga ttttaacgat
     1021 ttgccagatt gaggtgatat atgcctgatg ttcgtatgcc tgacggcact atcattaaaa
     1081 atgtgcctca aggcacaaca aaagcccagt tgatggctaa gttgcaaaaa aaacaacagc
     1141 aacagcaagt tgaaaagcct tatagtgatg gtaaagtggc tttgtcatct gtggttaaag
     1201 gcttggcttc tattcctgat gctgtattaa atacacctgc caacctaatg aatttaggca
     1261 aggccgcaac tggttatgta atgaatgaat caggctatgg cgatttagca caaagacttg
     1321 gtt
//
LOCUS       DAOQMB010000662         1318 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_86985_length_1318_cov_3.451306, whole genome shotgun sequence.
ACCESSION   DAOQMB010000662 DAOQMB010000000
VERSION     DAOQMB010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86985_length_1318_cov_3.451306"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="PLY05_12985"
     CDS             <1..490
                     /locus_tag="PLY05_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04253.1"
                     /translation="GKDWPLPRTGISQLLNPSPNQLSNLAKAKINPVILDKFSDGSRY
                     TFRDSLSMARTLVSKKKLNSVADMSTDLNQRIVRIVKDHLQKGMTVAIARSRAQIERL
                     LKNAEASGWLVPSRDPMMLGLPYTLELQPNEERPNDWLDVRCAVSFDGVARVAIIEQT
                     IS"
     gene            503..1264
                     /locus_tag="PLY05_12990"
     CDS             503..1264
                     /locus_tag="PLY05_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04254.1"
                     /translation="MSYLQDALRGRLLTAHEVEAVKSGKTRQRFDGANEKDGKEYNLT
                     LDKIVAIAAMAQIRTQAAHAVQVWAETDDLDKGETLYDRLDALISYIADGGIDLDDDP
                     ATDDRDDIYMVALQAASDYMSALGADDADLETLFNSKDDDARDEVAQRLVEFLMQTLG
                     EDEDAAEDAVQNFAFDLESESDVFDSVATRTVRRVRKGKIVTIKQRIRTTKPILSQKM
                     KDHLAKLHAKGITAKVAKRISKSLKLGHKNGLYKK"
BASE COUNT          412 a          242 c          311 g          353 t
ORIGIN      
        1 aggtaaagat tggccattgc cgcgtacagg tattagtcaa ttattaaatc catcgcctaa
       61 tcaattaagt aatttggcta aagctaaaat caatccagtg attttagata agtttagtga
      121 tggcagtcgt tacaccttcc gcgattcgtt gtctatggcg cgtactttgg tgagcaagaa
      181 aaagctaaac agcgtagcgg atatgtcaac agaccttaat cagcgcattg tgcgaattgt
      241 taaagaccat ttgcaaaaag gcatgacagt agctattgct cgcagccgcg cacaaattga
      301 acgcttatta aaaaatgcag aggctagtgg ttggctagta ccaagccgtg acccgatgat
      361 gcttggctta ccttatacct tagaattgca gcctaacgaa gaacggccta atgattggtt
      421 agatgtacgt tgtgcagtaa gttttgatgg tgtggcgcgt gtcgcaatta ttgaacaaac
      481 aatctcttaa taggggcaag atatgagtta tttacaagat gcgttacgtg gtcgcttact
      541 taccgcgcat gaagttgaag cagtaaaaag tggtaaaaca cgtcaacgct ttgatggtgc
      601 taacgaaaaa gacggcaaag agtacaattt aactttagac aaaattgttg cgattgcagc
      661 aatggcgcag attcgtactc aagccgccca tgcggtgcaa gtatgggcag aaacagacga
      721 tttagacaaa ggtgagactt tgtatgaccg tttagatgcg ttaattagct acattgcaga
      781 cggtggcatt gatttagacg atgacccagc aaccgatgac cgtgacgata tttacatggt
      841 agctcttcag gcagccagtg actatatgtc ggcattaggt gcagatgatg cagacttaga
      901 aactttgttt aacagcaaag acgatgatgc acgcgatgaa gttgcccagc gtttagttga
      961 atttttgatg caaacattag gcgaagatga agacgcggca gaggatgccg tacaaaactt
     1021 tgcgtttgac ttagaaagcg aaagtgatgt gtttgatagt gttgccactc ggactgtgcg
     1081 ccgtgttcgc aaaggtaaaa ttgtgactat taagcaacgt attcgcacta ctaagccaat
     1141 tttgtctcaa aaaatgaaag accacttggc taagttacac gctaaaggca ttactgccaa
     1201 agttgcaaaa agaatctcaa agtctttaaa acttggccac aaaaacggac tttataaaaa
     1261 ataaaggtag tctgctgctt aaaaaaggtt acgtcaaaag cgtgaccttt tttgtttt
//
LOCUS       DAOQMB010000663         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87205_length_1316_cov_14.798573, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000663 DAOQMB010000000
VERSION     DAOQMB010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87205_length_1316_cov_14.798573"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PLY05_12995"
     CDS             complement(<1..1280)
                     /locus_tag="PLY05_12995"
                     /inference="COORDINATES: protein motif:HMM:NF027305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HNC04255.1"
                     /translation="MNGGALVLPVLHELIWVNMNTLRLEVKREKQESLITKDKMRVDV
                     GVEFYVRVNPTEEAIAAAAQTLGARTLNANELRTLIEGKFVAALRACAAQMDMAEMHE
                     HRSRFVQSVQSIVVEDLAKNGLELESSSLTHFNQTKVEYFDPQNAFDAEGLTRLTEQT
                     ENRRKERNNIEQETAVSIATKNLEANRQQLEIEQQNEFAKLAQEREIETRKAQQAAEI
                     AAQQADRQRESQQAQIAAKQQTDTSRIAAERTIKEQEVEAERKVTIAKIEKDKAIQIA
                     QQATNIEIAGKSRDESVAKAEADQARAEAVKAEEGVVTSRQIAVAEREKSIQLVEARK
                     KAEQDAIGITVAAEADRDAAKAKAESLQTQATAEAEADRIRAEGIRAKYAAEADGKRM
                     INEAENTLSPELIAMQVKMLLIKELPKIIEQSVKP"
BASE COUNT          284 a          303 c          275 g          454 t
ORIGIN      
        1 ggcttaaccg attgctcaat aattttgggt aattctttaa ttaacaacat cttcacttgc
       61 atcgcaatta actcaggcga taatgtattt tcagcttcgt taatcatgcg tttaccatct
      121 gcttcagcag catatttcgc gcgaatacct tcggcacgaa tccgatctgc ttcggcttcc
      181 gcagtcgctt gtgtttgtaa ggactctgct tttgctttag ccgcatcacg gtcagcttca
      241 gctgccacag taataccaat cgcatcttgt tcggcttttt tgcgtgcttc aaccaattga
      301 atagactttt cccgttcagc cactgcaatt tgacgtgaag tgacaacccc ttcttcggct
      361 ttcaccgctt ctgcacgtgc ttgatcggct tcggctttgg ctaccgactc atcacggctc
      421 ttgcctgcaa tctcaatgtt ggtcgcttgt tgagcaattt gaatggcttt atctttttcg
      481 attttggcaa tggtgacttt gcgttcggct tcaacttctt gctctttaat ggttcgttct
      541 gcggcaatac gcgaagtatc tgtttgttgt ttggcagcaa tttgcgcttg ttgtgattca
      601 cgttgtctgt cagcttgttg tgctgcaatt tcagctgctt gttgggcttt acgggtttca
      661 atttcccgtt cttgggcaag tttagcaaat tcgttttgtt gttcaatttc aagctgttga
      721 cgattggctt ctaggttttt tgtggcaata gatactgccg tttcttgttc aatgttatta
      781 cgctctttac gacgattttc ggtttgttcg gtgagtcggg ttaaaccttc ggcatcaaac
      841 gcattttgtg ggtcaaagta ttccactttg gtttggttaa agtgggtgag ggaagagctt
      901 tctaattcca aaccgttttt agctaagtct tcaaccacaa tggattgcac gctttgcaca
      961 aaacgagaac gatgttcgtg catttcggcc atatccattt gtgccgcaca cgcacgtaag
     1021 gcagcgacaa atttaccttc aattaatgtg cgtaattcat tggcattaag tgttcttgcg
     1081 cccaaggttt gagcggctgc ggcaatcgct tcttcggtag ggttaacacg cacataaaac
     1141 tcaacaccca catctacgcg cattttgtct ttggtaatta atgactcttg tttttcacgt
     1201 ttaacttcta aacgtaatgt attcatgttg acccaaatca gctcatgtaa tacgggtaaa
     1261 actaaggcgc caccattcat aatcactgct tggccaccaa aacctgttct cacaaa
//
LOCUS       DAOQMB010000664         1316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87206_length_1316_cov_14.739889, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000664 DAOQMB010000000
VERSION     DAOQMB010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1316
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87206_length_1316_cov_14.739889"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLY05_13000"
     CDS             <1..726
                     /locus_tag="PLY05_13000"
                     /inference="COORDINATES: protein motif:HMM:NF039651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6091 family protein"
                     /protein_id="HNC04256.1"
                     /translation="TFAGSLESIGSIPSYKHLKVVVAAISSPKAANLMKQGDYEVGGV
                     MPLGSAYLFLRDRSIDEVGKLAGKKIAVFDYDKSQSQLVHGVGAQAIPSDISNFAAKF
                     NNGVVDVIAAPATAYRPLELYRGLGQNGGIPDFLVAQVFLQVVLRHERFPEGFGQKSR
                     SYLSSQFDRVLAIVQRYEKDIDPKYWMHIPDEDRPHYVALMRQARIDLTNNGTYDKRM
                     MSLLKKVRCQVEPTNSECSDNLE"
     gene            883..>1316
                     /locus_tag="PLY05_13005"
     CDS             883..>1316
                     /locus_tag="PLY05_13005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04257.1"
                     /translation="MNQKPVFMNRSLSEWLSSLPVFLLLILTLVIGTGEMIHGQLLRM
                     GEKLFGDPSTGVQYFMLRADPTKPDCDPNPDIEAEVQKKVAGGGADDEFGGLFADEVV
                     DPEAVRQSLTTARDECVEKFKTYESLVKHVTPELKAYRTFETA"
BASE COUNT          374 a          227 c          311 g          404 t
ORIGIN      
        1 acctttgcgg gtagtttgga gtcgattggc tcaattcctt catataaaca cttaaaagtt
       61 gtggtggctg cgattagtag tccaaaagca gccaacttaa tgaaacaagg tgactatgaa
      121 gtgggtggcg tgatgccatt gggatcggct tatttatttt tgcgtgatcg ctcaattgat
      181 gaagtgggta agttggcagg caaaaagatt gcagtgtttg attatgataa atcgcaatct
      241 caattagtgc atggtgtggg tgcgcaagcg attccttcgg atattagtaa ttttgcagct
      301 aagtttaata atggtgtggt tgatgtgatt gccgcacctg caacagccta tcgtccttta
      361 gaactgtatc gtggtttagg tcaaaatggt ggtattcctg attttttagt tgcccaagta
      421 tttttacaag tagtgctgcg tcacgaacgc tttccagaag gttttgggca aaaatcacgt
      481 agttatttat cttcacagtt tgatcgtgtg ttagcgattg tgcaacgtta tgaaaaagat
      541 atagacccta aatattggat gcatattcct gacgaagatc gaccacatta tgtggcgtta
      601 atgcgtcaag cacgaattga tttaaccaat aacggcactt atgacaaacg aatgatgtct
      661 ttattaaaaa aagtacgctg tcaagttgaa cccactaatt ctgaatgtag tgacaattta
      721 gaataattta tttaagtttt tatgagtgtt gctgtgcagc attcatgatg gggaagcatt
      781 atttagtcat aaataatgtt cgttgtagtc gacaagtaag aggagtttag gctgactcag
      841 gggtgttact ttgcagtcta aattcttaag aagcaggaaa acatgaacca gaaacctgta
      901 tttatgaacc gttccttatc ggaatggtta tcgagtctgc ctgtctttct cttgctcatc
      961 ttaacattag tcattggtac gggtgaaatg attcacggcc aattgttaag aatgggtgaa
     1021 aaactctttg gtgaccccag tacaggggtt cagtatttca tgctgcgtgc tgacccaacc
     1081 aaaccagatt gtgatcctaa tcctgatatc gaagccgaag tgcaaaaaaa agttgcaggt
     1141 ggtggcgcag atgatgagtt tggtggtttg tttgccgatg aagtggtaga cccagaagcc
     1201 gtacgtcaat ctttaaccac tgctcgtgat gagtgcgtgg aaaaattcaa aacctatgaa
     1261 agtttagtca agcacgtcac gcctgaatta aaagcctatc gcacctttga aaccgc
//
LOCUS       DAOQMB010000665         1315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87439_length_1315_cov_2.417460, whole genome shotgun sequence.
ACCESSION   DAOQMB010000665 DAOQMB010000000
VERSION     DAOQMB010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87439_length_1315_cov_2.417460"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLY05_13010"
     CDS             complement(<1..391)
                     /locus_tag="PLY05_13010"
                     /inference="COORDINATES: protein motif:HMM:NF037921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04258.1"
                     /translation="MLANQPKNDTLYIKGFNIVVENRAFTYRQVAQNVGQKACLMPAH
                     YGYLQNNPKVGVFVGAFPENDTVYLINQKAQGGYFDKHIAMFGFLSEQQAVDTYQKCF
                     EYGYIGLMSVVTCTIEQLKWWLANGDKS"
     gene            complement(476..952)
                     /locus_tag="PLY05_13015"
     CDS             complement(476..952)
                     /locus_tag="PLY05_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04259.1"
                     /translation="MLNEVPLAIRRALRAVTLNHPNRMGCTVYRKVVTRSAPDTFGGL
                     PTLGGLGVLDNEDETDFEYQELGDASLVFLQLYQSGFRSDDNSLLDNPNTPQQEASIE
                     PINENEFTPKKYDIVNVVIGNAILPYEITEISGTVNIPPFTQKYILQLRDELVQMV"
     gene            complement(962..>1315)
                     /locus_tag="PLY05_13020"
     CDS             complement(962..>1315)
                     /locus_tag="PLY05_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04260.1"
                     /translation="INLLPTWTMFFKEQDLKSAIDAYHNLDSNGLIELESGMERYSPV
                     GAIQINGIKETGIEYRFSELNNGHVAVLMCALFAEKQRNIRASLSAQVRVLHGSDNSR
                     SSDYVIKPISVGYLF"
BASE COUNT          376 a          283 c          246 g          410 t
ORIGIN      
        1 gtgacttatc gccattggca agccaccatt taagctgctc aatggtgcaa gtgactacgc
       61 tcattaggcc aatgtagcca tattcaaaac acttttgata agtatcaacg gcttgttgct
      121 cactcaaaaa gccaaacatt gcaatatgtt tatcaaaata gccaccttgc gctttttggt
      181 taatcaggta aaccgtgtca ttttcgggga acgcaccgac aaatacaccg actttaggat
      241 tgttttgtaa gtagccgtaa tgcgctggca ttaaacaggc tttttgacct acgttttggg
      301 caacttgacg ataggtaaag gcgcgatttt caacaacaat gttaaaacct ttaatgtaca
      361 acgtatcgtt tttaggttga ttggcaagca taactaagca cctagtattt tttgaattgt
      421 gcttagtgta aatcagtttt gatggggtat ttttgagggg ttacatgacg caaccctaga
      481 ccatttgcac taattcatct cttagttgca aaatatactt ttgggtaaag ggcggtatat
      541 tgactgtgcc tgaaatctcg gtaatttcgt aaggcaaaat tgcattgcca ataaccacgt
      601 ttacaatatc gtatttttta ggggtaaatt cgttttcgtt aattggctca atagaggctt
      661 cttgttgtgg cgtgtttgga ttatctaaaa gcgagttatc atcacttcta aagccacttt
      721 gatataactg taaaaacact aagctcgcat cgcctaattc ttgatattca aaatcggttt
      781 catcttcatt gtctaatacg cctaaaccac caagtgtcgg caatccacca aaggtatcag
      841 gcgcggagcg tgtcactact ttacgataga ccgtacagcc catacgatta ggatgattca
      901 aagtcacggc tcttaatgcg cgtcttatcg ctaaaggtac ttcatttaac atcgttcaca
      961 cctaaaataa ataaccaaca cttatcggct taatcacata atcactgctg cggctattat
     1021 cgctaccgtg taatacccgt acttgtgccg acaaactcgc tctaatgtta cgctgctttt
     1081 cggcaaataa cgcacacatt agcactgcca catggccatt gtttaattcg ctaaaacggt
     1141 attcaatacc tgtttcttta ataccgttaa tttgaatcgc accaactggc gagtaacgct
     1201 ccatgcctga ttcaagctca atcaagccat tgctgtctaa attgtggtaa gcatcaatcg
     1261 cgctttttaa gtcttgctct ttaaaaaaca tcgtccacgt tggcaatagg ttaat
//
LOCUS       DAOQMB010000666         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87610_length_1313_cov_8.434817, whole genome shotgun sequence.
ACCESSION   DAOQMB010000666 DAOQMB010000000
VERSION     DAOQMB010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87610_length_1313_cov_8.434817"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1202
                     /locus_tag="PLY05_13025"
     CDS             279..1202
                     /locus_tag="PLY05_13025"
                     /inference="COORDINATES: protein motif:HMM:NF020481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HNC04261.1"
                     /translation="MNLPYYHPDVRNLAWILAAPSLLKFLPEFNQPLTVLDDQFWQQQ
                     YQAYLPRLQQLEDNPLILEQFLAEHNNHRLGYYFEYLLLFWLQDNQYHCFELIKHRAT
                     LFKGKITIGELDYLVRNKITGEIEHWEVAIKFYLGYPPLTDAYHWLGANDNDSLGRKL
                     HHLATKQFLADQYQDYKINKRCLLVKGRLFYPSTAKNLLARAHSPTVSCLSPNHLQGN
                     WIMYQDFINSPEAAQLHWRHVAKDEWLADQQPFKQLPLANSQHLPPLPTARAELFLGF
                     NAQLEEQARCFVCGVPRAIHLLASTHFGCAQ"
BASE COUNT          386 a          250 c          249 g          428 t
ORIGIN      
        1 aaattgtgcc caatgatttt tcattttttc atctggatca ctattacgtt gctcttcagc
       61 aattgataag cactccataa attgatcggc tttatcactg gcttcgtctg cataactcac
      121 ggtatttaaa cagcttatta atgctaaggc gcaaagggag tatttcatga tggtagtctc
      181 tggtttatag tgatgattta taataattgg cagaatgttt tttaatcact atatacataa
      241 ttattaaatt catccacaaa aaagctgagg atttaagatt gaatttgcct tattaccatc
      301 cagatgtacg caatttagca tggatcttag ccgcacctag tttattaaaa tttttacctg
      361 aatttaatca gcccttaact gtgttggatg atcaattttg gcagcaacaa tatcaggcat
      421 atttaccacg tttgcagcaa cttgaagaca atcccctcat tttagaacag tttttggcgg
      481 agcataataa tcatcgttta ggttattact ttgagtattt gttattgttt tggttacaag
      541 ataaccaata tcactgtttt gagttaatta agcatcgagc aacgctgttt aaaggaaaaa
      601 ttactattgg cgaactggat tatttggtta gaaacaaaat aacaggcgag attgaacatt
      661 gggaagtggc cattaaattt tatttaggtt atccaccact gactgatgcc tatcattggt
      721 taggagcaaa cgataacgac tctttaggac gaaaattgca tcatttggcg actaaacaat
      781 ttttggctga tcaatatcag gactacaaaa ttaataaacg ttgtttgcta gttaaaggac
      841 gattatttta tccctcaacc gctaaaaatc tcttagcacg cgcgcacagt ccaacggttt
      901 cttgtttatc acccaatcat ttacaaggta attggatcat gtatcaggac tttattaaca
      961 gccccgaagc tgctcaattg cactggcggc atgtggccaa agatgagtgg ttggccgatc
     1021 aacagccctt taaacaattg ccattggcaa actctcaaca tttaccgcca ttaccaacag
     1081 cacgtgctga attattttta gggtttaatg ctcaattaga agaacaagcg cgttgttttg
     1141 tgtgtggtgt gccaagagct attcacttgt tggcttcgac tcacttcggc tgcgctcagt
     1201 gaccaccgct cagccaacgt aacgatgtac cctgagcgga gtcgaagggt tatgttttag
     1261 cggttttctg atgtactagc caacaatttt tgttggctat acctacataa taa
//
LOCUS       DAOQMB010000667         1313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87621_length_1313_cov_4.459459, whole genome shotgun sequence.
ACCESSION   DAOQMB010000667 DAOQMB010000000
VERSION     DAOQMB010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87621_length_1313_cov_4.459459"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..1150
                     /locus_tag="PLY05_13030"
     CDS             146..1150
                     /locus_tag="PLY05_13030"
                     /inference="COORDINATES: protein motif:HMM:NF006512.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HNC04262.1"
                     /translation="MTIKVGINGFGRIGRLALRAAWDWPEFEFVQINDPAADAATHAH
                     LLNFDSVHGRWQHQADSDEQHILIGAKKIKVTQHKTISDTDWSGCDLVIEASGKHKTV
                     AVLQAYLAQGVKRVVVSAPVKEQGALNVVMGVNQHLYDPAQHSIVTAASCTTNCIAPV
                     IKVIHQHLGIRHGSITTIHDLTNTQSILDQAHKDLRRARACGMSLIPTTTGSATAIAE
                     IFPELRGKLNGHAVRVPLANASLTDCVFEVERATTIDEVNQLLKDASENDLKGILGYE
                     SRPLVSIDYRTDPRSSIIDALSTMVINGTQVKLYAWYDNEWGYANRTVELARLVGTHA
                     "
BASE COUNT          362 a          311 c          278 g          362 t
ORIGIN      
        1 gcacaacaac aaggccgttt tattttttat aaagccaatc tgtcgcaaat gaaaattttg
       61 atcgattttt taaccgataa ctgttgccaa gggcagcctt gtgcggtcac agccaataaa
      121 ttttgtggtt tatgaggagc aaacaatgac gattaaagtg ggtattaatg gttttggacg
      181 tattggccgt ttagctttac gtgcggcatg ggactggcca gaatttgaat ttgtacaaat
      241 caacgaccct gctgctgatg ccgccaccca tgcacattta ctcaattttg attcggtaca
      301 tggccgttgg cagcatcaag ccgatagtga tgagcaacat atcctcattg gtgctaaaaa
      361 aataaaagtc acccaacaca aaaccattag cgataccgat tggtctggct gtgatcttgt
      421 cattgaagcc agtggcaaac ataaaaccgt tgctgtgttg caagcctatc tcgctcaagg
      481 cgtaaaacgc gtcgtcgtca gtgcgcccgt caaagagcaa ggggcattaa atgtggtcat
      541 gggcgttaat caacatttat acgatccagc acaacacagt attgtgactg cggcttcgtg
      601 taccaccaac tgtattgcac ctgtgattaa agtgattcat caacacttag gcattcgtca
      661 tggttcaatc accaccatcc acgacctaac caacactcaa agcattttag accaagccca
      721 taaagacctg cgccgcgcgc gggcttgtgg catgagccta attccaacaa ccacaggttc
      781 ggccaccgcc attgccgaaa tttttcctga gctacgtggt aaattaaatg gtcatgcggt
      841 acgtgtgccc ttagccaatg cctcattaac cgattgtgtg tttgaagttg aacgcgccac
      901 taccattgac gaagttaacc aactgttaaa agatgcctca gaaaatgact taaaaggtat
      961 tttaggttac gaaagccgtc ctttggtatc tattgattat cgcaccgacc cgcgctcatc
     1021 cattattgac gcattatcga ccatggtaat caatggcaca caagtcaaat tgtatgcttg
     1081 gtatgataat gaatggggct atgccaatcg cacggttgaa ctcgctcgtt tggtaggtac
     1141 tcacgcctaa tcgcccaccc tctcattgtg agagggattt atttgatact cgataaatcc
     1201 atgctcaatc atctttctcc acagctcaaa caatacctga ttgtcacagg caattattgg
     1261 gcgtttaccc tcaccgatgg tgcattacgc atgttagtcg ttttgcactt tta
//
LOCUS       DAOQMB010000668         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87750_length_1312_cov_18.083532, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000668 DAOQMB010000000
VERSION     DAOQMB010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87750_length_1312_cov_18.083532"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="PLY05_13035"
     CDS             <1..990
                     /locus_tag="PLY05_13035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNC04263.1"
                     /translation="GFNRSIDASRQVGSLFKPAIYLTALATGKYNLVSPIDDGPVQVQ
                     GQSGDVWSPENYDHQSHGIVPLYDALAHSYNQATIRLGMSQDVGISAVINTLKQLGVT
                     QDLPNYPSLLLGTANLSPMEVLRLYQPFAANGFQSSPRTILEVVDAKGKQLKRNGLEV
                     KQVFDPAPLYLINYAMQQTMISGTAASAYQRLPKDLVMAGKTGTTNEARDAWFAGYTG
                     NYLAVVWLGNDDNKTTGLSGGTGALPIWTDIMSKLKPVSLQTVQPDSVQWQWLERSTG
                     KLSAEGCADAILVPIIADSVTATSDCALDKSPANDPINAIVNGSVNKVLDIFR"
     gene            999..>1312
                     /locus_tag="PLY05_13040"
     CDS             999..>1312
                     /locus_tag="PLY05_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04264.1"
                     /translation="MRAVYISLVALNLTACQHLADYSWPTNSAPIKANKPQVISKPIS
                     KPTSTIKQQPVVVAPVERVEPKDDLPVTTPTVTKPVLPPPVLVQPNKKLADGRDMPAV
                     QG"
BASE COUNT          379 a          257 c          297 g          379 t
ORIGIN      
        1 ggttttaatc gaagcattga tgcttcacgg caagttggct ctttgtttaa gccagcgatt
       61 tatttaaccg cattggcaac aggcaaatat aacttagttt caccgattga tgatgggcca
      121 gtacaagtgc aaggtcagag tggtgatgtt tggtcgcctg aaaattatga ccatcaatca
      181 catggcattg tgccattata tgatgcttta gcacattctt ataaccaagc aacgattcgt
      241 ttgggtatga gtcaagatgt tggcatttca gcagtgatta acacgctaaa acaattaggc
      301 gtcacccaag atttgcccaa ttatccatcg ttgttattgg gtacggcaaa tttatcgcct
      361 atggaagtat tacgtttgta tcaacccttt gcagctaatg gctttcaaag ttcacctcgg
      421 actattttgg aagtggtgga tgctaaaggc aaacagttga agcgtaatgg tttagaagtg
      481 aaacaagtct tcgatccagc acctttatat ttaattaact atgccatgca gcaaacgatg
      541 atcagtggta cggctgcttc tgcttatcaa aggctaccca aagatttggt gatggcaggt
      601 aaaacaggca caaccaatga agcacgtgat gcatggtttg caggttatac aggtaattat
      661 ttggctgtgg tatggttagg gaatgacgac aacaaaacca ctggtttatc aggcggaaca
      721 ggtgcattgc caatttggac cgatattatg agcaagctca aacctgtctc tttacaaacg
      781 gtacaacctg atagtgtgca atggcaatgg ttggagcgtt caacaggcaa gttgagtgcc
      841 gaaggctgtg ctgatgcgat tttagtgcca attatcgccg attctgtaac tgcaaccagt
      901 gattgtgcct tagataaatc gcctgctaat gatccgatta atgcgattgt aaatgggtct
      961 gttaataaag ttttagatat ttttaggtga taatggtcat gcgtgctgtt tatatttcct
     1021 tagttgcgtt aaatctaacg gcttgtcaac atttggcaga ttatagttgg ccaacaaact
     1081 ctgcacccat taaagccaat aaaccgcaag tgattagtaa acctatcagc aagccaacgt
     1141 caacgattaa acaacagcct gtggttgttg cacctgtaga acgcgttgag cctaaagacg
     1201 atttaccagt caccacacca acagtcacta aacctgtgtt accgccacct gtgttggttc
     1261 aacctaataa aaaattggca gatggtcgag atatgcctgc ggtacaaggt tt
//
LOCUS       DAOQMB010000669         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87751_length_1312_cov_14.249801, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000669 DAOQMB010000000
VERSION     DAOQMB010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87751_length_1312_cov_14.249801"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="PLY05_13045"
     CDS             <1..149
                     /locus_tag="PLY05_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019458128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04265.1"
                     /translation="GNPVDKFTKTWDVYYKFTLKLMNLKTGLVEWQDEQEIRKTAKRG
                     LLGM"
     gene            complement(221..>1312)
                     /locus_tag="PLY05_13050"
     CDS             complement(221..>1312)
                     /locus_tag="PLY05_13050"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HNC04266.1"
                     /translation="EKMGKEYLTNRGLPADGNIYKAEDFEFVLNDNLTLDVKGAVLNK
                     DNFEKTLSLETDNKNHQMLIAMINALNDNSKDFDTIFGQYFDKSNYLTWLATSILMGN
                     RDTINQNFALYQPKDSSKFYFLPWDYDGSFGFEGQPDQKKAGDLYAPWQLSVANWWSV
                     PLHKRFLKDPKHLAELKQAVEEVYSKYLTEAKIQAKLDAYKPLIQAVISTTPDKTFLP
                     VVDDVGFLQEWQGEYTRIAKVPKQNYEHFFASLESPMPYYQAVELLSDGTLRLGWDVA
                     TDLQGDTVSYTVQWSTSPNFNTVLGQQSVTTTEIIMPKLPNGVYYLKVTAKDSKGNSQ
                     NAFDTATVAGKKYFGVYAFEVKAGEVVAL"
BASE COUNT          392 a          286 c          221 g          413 t
ORIGIN      
        1 caggcaatcc tgtcgataaa tttaccaaaa cttgggatgt ttattataaa tttacactca
       61 aattaatgaa tctaaaaaca ggcttagtcg agtggcaaga cgaacaagaa attcgtaaaa
      121 cggctaaacg cggtttgttg gggatgtgag tttttagacc tcaccctagc cctctcctaa
      181 caggagaggg aattttccat cgttataatt ttctcattgt ttacaacgcc accacttccc
      241 ctgccttaac ctcaaacgca tacacaccaa aatacttttt gcctgccact gtcgcggtat
      301 caaaggcatt ttgactgtta cctttactat ctttagcagt gacttttaaa taatacaccc
      361 cattaggtaa ttttggcata ataatttcgg tagtcgttac gctctgttga ccaagtacgg
      421 tattaaaatt tgggctagtt gaccactgca cggtatagct cacagtatcg ccttgtaggt
      481 ctgtagctac atcccacccc aaacgtaaag tgccatcaga taataactct accgcctgat
      541 agtacggcat cggactttct aaactcgcaa aaaaatgctc atagttttgt ttaggtactt
      601 tggcaatacg cgtatattcg ccctgccact cctgtaaaaa gccgacatca tctactacag
      661 gtaaaaaagt tttatcaggc gtggtactaa taacagcttg tattaaaggc ttgtaagcat
      721 ctaatttggc ttgaattttg gcttcggtca gatatttact ataaacttct tcaaccgctt
      781 gtttgagttc ggccaaatgc ttggggtctt ttaaaaagcg tttatgtaat ggcacactcc
      841 accaattagc cacacttaat tgccacggag catacaaatc ccctgctttc ttttggtcgg
      901 gctgaccttc aaaaccaaaa gatccatcat agtcccacgg taaaaaataa aatttactgc
      961 tatctttggg ttgatacaaa gcaaagtttt gattaatggt atcgcggttg cccattaaaa
     1021 tactggtggc caaccaagtt aaatagttac ttttatcaaa atactgacca aaaatagtat
     1081 caaaatcttt gctgttatca ttgagagcgt taatcatcgc aatcagcatt tgatggtttt
     1141 tattgtcggt ctccaaactt agcgtttttt caaaattatc tttattaagc accgcacctt
     1201 tgacatctaa tgttaagtta tcatttaaca caaactcaaa atcttcggct ttataaatat
     1261 taccatcagc aggcaggcca cgattagtca aatactcttt acccattttt tc
//
LOCUS       DAOQMB010000670         1312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_87752_length_1312_cov_10.797932, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000670 DAOQMB010000000
VERSION     DAOQMB010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87752_length_1312_cov_10.797932"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..369)
                     /locus_tag="PLY05_13055"
     CDS             complement(64..369)
                     /locus_tag="PLY05_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005410145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04267.1"
                     /translation="MTITLDIIGGKWKGMILYHLVSGTRRFNELMRLMPDVTQRMLTR
                     QLRELEDDGVIIRKVYAEVPPKVEYSLSEVGQTLLPVIHSLSQWGKMYMTYTNPQKE"
     gene            625..1239
                     /locus_tag="PLY05_13060"
     CDS             625..1239
                     /locus_tag="PLY05_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC04268.1"
                     /translation="MNVFTAVENRRSVKQFNPDHQMTLDEKQKLLSATLLSPTSFNIQ
                     NWRFVLVEDLALRQQLKAAAWNQAQITDASLVVVICGDLNAWQKEPLRYWRNLEDVAI
                     RDYILNALHNFYVARPQVQRDEVMRSAGIAAQTLMLSAQALGYDSCPMIGFNVDEVAK
                     LINLPEDHEIGMIVTIGKALQPARPRSGALAFEEVVIKDQFAQA"
BASE COUNT          378 a          280 c          266 g          388 t
ORIGIN      
        1 ccgttttgtc agacgtttat ttgtcaaaat gacagggcaa tcggccaagg cttttcggca
       61 acattactct ttttgagggt tggtgtaagt catatacatc ttgccccatt ggcttaaact
      121 atgaataaca ggcaacaagg tctgccccac ttcgctcaag gaatattcaa cttttggtgg
      181 tacttcggca tataccttac gaataatcac cccatcgtct tctagctcac gtaactgacg
      241 ggtcaacatt cgttgggtga catcgggcat cagtcgcatt aactcattaa aacggcgtgt
      301 accactgact aaatgataca aaatcatccc tttccacttg ccaccaataa tgtccaaagt
      361 gatggtcatt ggacaacgaa caatcgtgca ttcttcttta agcaacaatt gactcacttg
      421 ttgttgtact gcgcccatat tgcctcccaa tagtatacaa gatgtgtgta taagctattt
      481 atgtatttat atgctaaaaa tgtgcattct atcgctaatg aatgtattgc gctagtatct
      541 ggctactgat tcagtcaacg caaatttata gacaagcaaa cagtagtgaa tcgttattca
      601 actgcatgtc actaggagcc aaatatgaac gtctttaccg ctgtcgaaaa ccgtcgctca
      661 gtaaaacaat ttaatcccga tcatcaaatg actctggatg aaaaacaaaa attgttgtct
      721 gccactttat taagtccaac ctcttttaat attcaaaact ggcgttttgt gttggttgaa
      781 gaccttgcct tacgccaaca acttaaagct gccgcatgga atcaagcaca aattaccgat
      841 gctagtttag tggtggtgat ttgtggcgat cttaatgcgt ggcaaaaaga gccattacgt
      901 tattggcgca atttagaaga tgtggccatt cgtgattata ttttgaatgc cttacacaat
      961 ttttatgttg ccagaccaca agtgcaacgg gatgaagtga tgcgctccgc aggcattgcc
     1021 gcacaaacct tgatgctcag tgcgcaagcc ttgggttatg actcttgtcc aatgattggt
     1081 tttaatgtgg atgaagtggc caaactgatt aacctacccg aagatcatga aattggcatg
     1141 attgtcacca ttggtaaagc cttgcaacct gccagaccac gcagtggtgc tttagctttt
     1201 gaagaagtgg tgattaaaga tcaatttgct caggcttaag gcgtaaaggg gctgcatcag
     1261 ccgttccata aaacttatca aaacctactt attgtagaac aaataggctt tg
//
LOCUS       DAOQMB010000671         1310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_88143_length_1310_cov_2.354582, whole genome shotgun sequence.
ACCESSION   DAOQMB010000671 DAOQMB010000000
VERSION     DAOQMB010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88143_length_1310_cov_2.354582"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLY05_13065"
     CDS             complement(<1..485)
                     /locus_tag="PLY05_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HNC04269.1"
                     /translation="MIKPLFLKLSALPRSAKRYILMLADFIVFPLLLWFALFLRLGDT
                     GGISLLSEYPSSFLFVSVFCVVSLKAFSVYRAVVRSFEEKFLQELVWSVGCIVLVLFL
                     VASFHLLPLPRSTPFTFGFFVFLWVWGTRSTIRFLVRLFLQLQIPVSRIAIYGAGVAG
                     R"
     gene            complement(525..>1310)
                     /locus_tag="PLY05_13070"
     CDS             complement(525..>1310)
                     /locus_tag="PLY05_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNC04270.1"
                     /translation="HLAGWPCEMTAINELAKAHNLIVIEDCAQAHGATYKGQAVGGLA
                     DAAAWSFCQDKIMTTGGEGGMLTTNNEVLWSKAWSYKDHGKSWDAVYNQEHPSGFRWL
                     HESFGTNWRMLEMQAVVGSIQLKRMSDWHAARTHNATQILSACAKSHALRVPAVPDYI
                     KHAWYRAYAFVRPEALATGWSRERILAEINALGVPCYSGSCSEVYLEKAFDNTAWRPI
                     ERLPVAKKLGETSLMFLVHPTLTTLEVEKTCQAINSVMAQAQK"
BASE COUNT          428 a          314 c          240 g          328 t
ORIGIN      
        1 tggcgacctg ctacccctgc accataaata gcaatacgcg aaacagggat ttgcaattgt
       61 aagaataatc gcaccaagaa cctaatagtc gaacgagtac cccataccca caagaataca
      121 aaaaaaccaa aggtaaaggg tgtgctacga ggtaagggca gtagatgaaa agaggctact
      181 aaaaacaaga ctaaaactat acaacccaca gaccacacta attcttgtaa aaacttctct
      241 tcaaaagagc gtacaactgc cctatataca gaaaatgctt ttaagctaac aacacaaaac
      301 acactaacaa ataaaaaact tgatggatac tccgataata acgatatccc ccccgtatca
      361 ccaagtcgca aaaacaaagc aaaccaaagt aataatggaa acacgataaa atcggctaac
      421 attaaaatat atcgcttagc cgaacgaggc aaagcggata gctttaaaaa aagaggcttt
      481 atcacgtgat tattttcctt acattcttac tgaaaggcaa cgcattattt ttgcgcttga
      541 gccataacac tgttaatagc ttggcaggtt ttttcgactt caagtgtggt gagtgtagga
      601 tgcaccaaaa acatcaaact cgtttcgccc aattttttag ccacaggcag acgctcaata
      661 ggccgccaag cggtgttgtc aaaggctttt tctaaataaa cttctgagca tgagccacta
      721 tagcaaggca cacccaatgc attaatttcg gccaaaatac gttcacgact ccaacctgtt
      781 gctaacgcct ctggacgcac aaaagcatag gctctatacc aagcgtgttt aatataatct
      841 ggcacggcag gaacacgcaa tgcatggctt ttggcacatg ctgataaaat ttgtgttgca
      901 ttgtgtgtgc gggcagcatg ccagtcactc atacgtttga gctgaatgct acctacaaca
      961 gcttgcattt ctaacatacg ccagtttgta ccaaaagatt catgtaacca tctaaatcca
     1021 gacggatgct cttgattgta aacagcgtcc cagcttttac cgtgatcttt ataagaccat
     1081 gcctttgacc acaacacttc gttgttagta gtcagcatac cgccctcacc acctgtggtc
     1141 ataattttat cttggcaaaa agaccacgct gccgcatctg ctaaaccacc aactgcttgc
     1201 cctttataag ttgcaccgtg tgcttgagcg cagtcttcta taactattag gttatgagct
     1261 tttgctagct cattaatggc agtcatttca catggccaac ctgccaagtg
//
LOCUS       DAOQMB010000672         1307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_88435_length_1307_cov_17.758786, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000672 DAOQMB010000000
VERSION     DAOQMB010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1307
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88435_length_1307_cov_17.758786"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1307)
                     /locus_tag="PLY05_13075"
     CDS             complement(<1..>1307)
                     /locus_tag="PLY05_13075"
                     /inference="COORDINATES: protein motif:HMM:NF024547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HNC04271.1"
                     /translation="PIKQSLAPVMPFSADLLPHTLREFVMDESDRMPCPPDFVAVSIL
                     TTLGSTIGASCGIKPKQKDDGWIIVPNQWGGIVAPPTSLKTPAMEAGIKPLNKLIEEA
                     IKNHESELKTHKRDELAYQARLEGLRADLKSASKTTARKKAEHTANELVDLIMEAEDN
                     APPPPVMRRYLTNDATIEKLGELERDNPNGILLMRDELMGWLAGLEKEGKQEDRAFYL
                     EGFNGTGSKVVDRIMRGSTFIENHCLSVLGGIQPDKLIAYFEQQISGLGNDGLLQRFQ
                     LLVYPDAMTWGYRDRYQNKDALNAILELFKKLSESDFVEFGAYPTDEYNKRPYFRFTL
                     DAQAFFIEWLKKLNTEIIANEENTLIQQHLGKYARLMPSLALIFHLVDCVTHNTQGQV
                     GLESAKQAVNWCEYLETHARRVYGLFDGMGMRPALTLSKKIEAL"
BASE COUNT          327 a          298 c          273 g          409 t
ORIGIN      
        1 agtgcttcta tttttttaga aagcgtcaag gcggggcgca ttcccatacc atcaaacaag
       61 ccatacacgc gccttgcatg agtttctaaa tactcgcacc aattgacggc ttgttttgcc
      121 gactctaaac cgacttgccc ttgtgtgttg tgagtgacgc aatcgacaag atgaaagatt
      181 aaggcaaggc taggcatcaa tcgggcatac ttgcctagat gttgctgaat aagtgtattt
      241 tcctcattgg cgattatctc ggtattgagt tttttaagcc attcaataaa aaaggcttga
      301 gcatctaagg taaaacgaaa gtaagggcgt ttgttgtatt catctgtcgg gtacgcgcca
      361 aactcaacaa aatcactctc tgatagcttc ttgaatagct ctaaaatggc gtttagtgcg
      421 tctttgtttt gatacctgtc tctatagccc catgtcattg catcggggta aacaagcaat
      481 tgaaaacgct gtaacagtcc atcattacca agccctgaga tttgttgctc aaaatatgca
      541 atcagtttgt cgggttgaat gccgcctaaa acagataaac agtgattttc aataaatgta
      601 ctgcctctca ttatgcggtc aactaccttg ctgcctgtgc cgttaaagcc ctctaaataa
      661 aaggctcggt cttcttgttt accttctttt tcaagccctg ctaaccatcc catgagttca
      721 tcgcgcatta acaaaatacc gttgggattg tcacgctcta actcccctaa tttttcaata
      781 gtggcatcgt tggttaagta cctacgcatc acgggcggcg gtggtgcgtt atcttcggct
      841 tccatgatta aatccacaag ctcattagcc gtatgttctg cttttttgcg tgcggttgtt
      901 tttgacgcgc ttttcaagtc ggcgcgtaag ccctctaacc ttgcctgata cgctaattca
      961 tctcttttat gtgtttttaa ctctgactca tggtttttta ttgcttcttc aatgagtttg
     1021 tttaatggct tgattcctgc ctccatagca ggtgttttta atgatgttgg tggcgcaaca
     1081 ataccgcccc attgattagg aacaattatc catccgtcat ctttttgctt gggtttaatg
     1141 ccgcaacttg caccgatagt gctaccaagt gtcgttaaaa tactgactgc cacaaaatcg
     1201 ggagggcatg gcatacggtc gctttcatcc atgacaaact ctcgcaaggt atgaggcaat
     1261 aaatcagcac taaacggcat cacgggggca agactttgct ttatggg
//
LOCUS       DAOQMB010000673         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_88812_length_1304_cov_20.465172, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000673 DAOQMB010000000
VERSION     DAOQMB010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88812_length_1304_cov_20.465172"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="PLY05_13080"
     CDS             complement(<1..597)
                     /locus_tag="PLY05_13080"
                     /inference="COORDINATES: protein motif:HMM:NF023154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HNC04272.1"
                     /translation="MIRHTSNKNLTLLSTRLWRDLRVQSESEDLIVNYLDRIFDVLNN
                     AINEHPRTFVIRCELNFPSDYYEPDTAVISRFTDSLKAQIKAHEDRKRREGKRVHDCH
                     LRYIWVKERASSHVWHYHVALFVNRDAYFTLGLFKSGNRFSESSISESDNTNMSDRIR
                     MAWASALDLRLDETRGLVHFPKNPTYSLDVNDESFDDEF"
     gene            complement(1117..>1304)
                     /locus_tag="PLY05_13085"
     CDS             complement(1117..>1304)
                     /locus_tag="PLY05_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HNC04273.1"
                     /translation="LNEVMDCTGLGKSSIYKFMAAGTFPKAIPLGQRAVGWLEDEIKE
                     WITNRVKERNEIVTKKK"
BASE COUNT          434 a          265 c          183 g          422 t
ORIGIN      
        1 aaattcatca tcgaaacttt catcattgac atctaaacta tacgttggat ttttagggaa
       61 atgaactaat ccacgggttt catctaaacg caggtctaat gcactagccc atgccattct
      121 gatacgatca gacatattgg tattatcaga ttcactaata gatgactctg aaaaccgatt
      181 accagattta aacaatccca aggtaaagta agcatcacga ttaacgaaaa gagcaacatg
      241 gtaatgccag acatgggaac tggctcgttc ttttacccaa atataacgca agtggcaatc
      301 atgaacacgc ttcccttcac gacgcttgcg atcttcatga gctttgattt gagctttcag
      361 cgaatctgta aaacgagaaa ttactgctgt atcaggctca taataatcgc ttggaaaatt
      421 caattcacaa cgaatgacaa aagtccttgg atgttcgtta attgcattat ttaacacatc
      481 aaaaatacga tcaagatagt tgacaattaa atcttcagac tctgattgaa cccttagatc
      541 tctccacaat cttgttgata ataaagtgag gtttttatta gatgtatgac gaatcacaac
      601 gatgctcctg tgtagtttta gtacacaaaa tctgcatcac tatttttatt taaatttcag
      661 atagttctgt tattaaactt aggctattca ttatttgtta taagacatca accatttatc
      721 aaaattgaga tattctcatc taaagtttct taatcactaa tcattaaaga atttcttttt
      781 cttattctta tttaaatcaa ataattgttg atgcttgtta gtgtgattat cgttactcta
      841 taattagtat aataaatata gctattacag acattgacat cacaatatag cccatcaggc
      901 actcagtcat attttgacaa acacttccgt tccccctgaa gaagaaatat gcaacatacc
      961 caaaactaat aaccacttgt tgattattct actcaggaaa taaaatatct attttacatg
     1021 tacaggctgc taccaactta actaaggacg gcataatagc ggccaagccg ctattactgc
     1081 cacatcatgt gaataattga aatatcaaaa ctcttactat tttttcttgg taactatctc
     1141 atttctttcc ttaactctat tggtaatcca ctctttaatt tcgtcctcta accagcctac
     1201 tgccctctga cctaatggta tagccttagg aaaagtgcca gctgccataa acttgtaaat
     1261 tgacgactta cctaagccag tacaatccat aacttcattt aaac
//
LOCUS       DAOQMB010000674         1304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_88815_length_1304_cov_10.333066, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000674 DAOQMB010000000
VERSION     DAOQMB010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88815_length_1304_cov_10.333066"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1304)
                     /gene="dnaE"
                     /locus_tag="PLY05_13090"
     CDS             complement(<1..>1304)
                     /gene="dnaE"
                     /locus_tag="PLY05_13090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013108033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNC04274.1"
                     /translation="DIDFCMDGRDRVIDYVANHYGREAVSQIITFGTMAAKAVVRDVA
                     RVQSKSYGLADRISKLIPKTPGISLEEARKEEALLNDLLSNPTERDYEDANEIWEMAL
                     KLEGITRGVGKHAGGVLIAPGKLTDFTAVYCDEEGKWVSQFDKDDVEQVGLVKFDFLG
                     LRTLTIIDWALVHINAKRQALGQEPLDINNIPLDDYKTYQLLQEGRTTAVFQLESRGM
                     KELLKKLKPSCFEDIIALVALYRPGPLESGMVDDFCNRKHGRAKVAYPDETYQHDCLE
                     PILKPTYGVIVYQEQVMQIAQEMAGYSLGGADMLRRAMGKKKPEEMAKERVKFMAGAT
                     GKGIDADLAGHIFDLMEKFAGYGFNKSHSAAYALVSYQTAWLKQHYPAEFMAAVLSAE
                     MHNTDNVVTFIEECRTMGLPIVSPDVNHSNYKFVPLNETIVY"
BASE COUNT          366 a          332 c          250 g          356 t
ORIGIN      
        1 cataaacaat ggtttcgtta agtggcacaa atttataatt agaatgattg acatcgggtg
       61 agacaatcgg cagccccatg gtgcggcact cttcaataaa ggtcacaaca ttgtcggtgt
      121 tgtgcatctc agcagaaagt accgcagcca taaattcggc aggataatgt tgttttagcc
      181 atgcggtttg ataagacact aaagcataag ctgccgagtg cgatttgtta aaaccataac
      241 ccgcaaattt ttccatcaaa tcaaagatat gaccagcaag gtcggcatca atgcctttgc
      301 ctgttgcccc tgccataaat ttaacgcgct ctttggccat ttcttcaggt ttctttttac
      361 ccatcgcacg acgcaacata tccgcgccac cgagcgagta tcctgccatt tcctgagcaa
      421 tttgcatgac ctgttcttga taaacaatca cgccataggt cggttttaag ataggctcta
      481 aacaatcatg ttgataggtt tcgtcaggat acgctacctt cgcgcggcca tgtttacggt
      541 tacagaagtc atcaaccatg cccgattcca aggggccagg gcgatacaaa gcgaccaaag
      601 cgataatgtc ttcaaaacaa gagggtttaa gcttttttaa cagctctttc ataccgcgcg
      661 attccaactg gaacaccgcg gtagtacgcc cctcttgtaa taattgatag gttttatagt
      721 catctaaggg aatattatta atatccaatg gctcttggcc aagggcttgg cgtttcgcat
      781 taatatgcac caacgcccaa tcaataatcg ttaaagtacg caatcccaaa aagtcgaact
      841 tgaccaagcc aacctgttca acgtcatctt tatcaaactg actaacccac tttccttctt
      901 catcacaata cacggcggta aaatcggtca acttccctgg tgcaatcaat acaccacccg
      961 cgtgtttacc tacaccccgc gtaatgcctt cgagtttgag tgccatttcc caaatttcgt
     1021 tggcatcttc ataatcacgc tcggtaggat tacttaacaa atcattgagc aaggcttctt
     1081 ctttacgcgc ttcttcgagt gaaatccctg gtgttttagg aattaattta gaaattcgat
     1141 cggccaaacc atacgattta ctttgcactc tcgcaacatc tcgtaccacc gctttggcgg
     1201 ccatcgtacc aaaggtgata atttgcgaaa ccgcctcacg accatagtga ttggccacat
     1261 aatcaatcac gcggtctcga ccatccatac aaaagtcgat atca
//
LOCUS       DAOQMB010000675         1302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89191_length_1302_cov_2.158781, whole genome shotgun sequence.
ACCESSION   DAOQMB010000675 DAOQMB010000000
VERSION     DAOQMB010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89191_length_1302_cov_2.158781"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLY05_13095"
     CDS             <1..412
                     /locus_tag="PLY05_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04275.1"
                     /translation="AKQQDTKNITRSFCAIKEDNIIGYYSLSNATVDINNLPQEITKR
                     YKLPKHDMPVVRLCRLAIDSKAQRQGIGEKLLIDALEKVLSVAKVSGCVGMVVDAKDQ
                     AAANYYQGYGFVPAPDNPLLLFMPLPTIQELFAE"
     gene            complement(450..>1302)
                     /locus_tag="PLY05_13100"
     CDS             complement(450..>1302)
                     /locus_tag="PLY05_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HNC04276.1"
                     /translation="KEARKDAVKSLLEQEKNELESLQQQLLELEEEHSGDEGLLEQGI
                     KAKDIAELWNNEILEAASVLYANDFKSYQQNEQSLNQLQEECKLLEASQLIVENLINK
                     GIKYSNTKQVTALAENFTDEQKNQLNQYISKQKQLNSLRKTLKTLFEDFSTKVCNKLS
                     LAADELPNSDNLKVLQQWQNTYEAIGQAKERVKLLEAALDKMAYDYYGVLTETQIKQL
                     VVDHKWLASLQKMIEGEVERVSQQLTQRVRELAERYAKTLPQLSQEVSMFEAQVNQHL
                     AKMGFEL"
BASE COUNT          354 a          275 c          234 g          439 t
ORIGIN      
        1 cgcaaaacaa caagacacta aaaatattac acgcagtttt tgtgcgatta aagaagataa
       61 tattattggt tattattcgc tgagtaatgc cacagtagat attaacaatt tgccgcaaga
      121 aattactaaa cgttataaat tacccaagca tgatatgcct gttgtacgct tatgccgctt
      181 ggcgattgat agcaaagcac aacgccaagg aattggtgaa aagctattga ttgatgcact
      241 tgaaaaagtg ttgtcagtcg ctaaagtatc gggttgtgta gggatggtgg tcgatgctaa
      301 agaccaagca gcagcgaatt attatcaagg ttatggtttt gtacctgcac cagataatcc
      361 tttactgcta tttatgccct taccaactat tcaagaactt tttgctgaat aaaacaaagg
      421 cggccttaag gccgcctttg ttttatcgtt taaagctcaa aacccatttt agccaaatgt
      481 tgattaacct gtgcttcaaa catagacacc tcttggctta attgcggcaa agttttggca
      541 taacgctccg ccaactcacg cactcgttgc gttaattgtt ggctcacacg ctcaacctca
      601 ccttctatca ttttttgcaa actagccaac catttatggt caaccaccaa ttgtttaatt
      661 tgcgtctcgg ttaatacacc ataatagtcg taagccattt tatccaaggc ggcctctagt
      721 aatttaacgc gctctttggc ttggcctatc gcctcgtagg tattttgcca ttgctgcaaa
      781 actttaagat tgtcgctatt gggcaactca tcggcggcaa ggctcaattt gttacacacc
      841 ttggtactaa agtcttcaaa caacgttttg agtgttttgc gtaagctgtt taattgcttt
      901 tgtttactaa tatattggtt taattgattt ttctgttcat cggtaaagtt ttcggcaagt
      961 gcagtgactt gtttggtatt ggaatactta atacctttat tgattaaatt ttcaacaatc
     1021 aattgtgagg cttctaaaag cttgcattct tcctgtagct gattcaggct ttgctcgttc
     1081 tgttgatagc tcttaaaatc attggcatac aaaacactgg cagcttctaa aatctcgtta
     1141 ttccataact cagcaatatc tttggcttta ataccttgct caagcaaacc ttcatcacca
     1201 ctatgctctt cttctagctc cagtaattgt tgttgtaatg actcaagctc atttttttct
     1261 tgctcaagca agctttttac ggcatccttc cgcgcttctt tc
//
LOCUS       DAOQMB010000676         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89222_length_1301_cov_10.276886, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000676 DAOQMB010000000
VERSION     DAOQMB010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89222_length_1301_cov_10.276886"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PLY05_13105"
     CDS             complement(<1..1039)
                     /locus_tag="PLY05_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017400168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNC04277.1"
                     /translation="MTAIQQIALVGNPNCGKTSLFNQLTGARQKVANYAGVTVERKEG
                     FLTLASGRRLRILDLPGAYSLEATSPDEAITRAICLGEFAGEPTPDLLVCVVDATNLR
                     LHLRFVLEVQALGRPMLLVLNMMDDAKRRGIQIDSAILSQRLGIKVIETAAVKRHGID
                     QLLASLDQQIPALAKPTLIPDHDIHQQVRDLLAETVVMPRDTDQRDDFLDSILLHPVA
                     GLAILGVLMFFIFQAVFAWAAPFMDGIETSVTWLSELIGQYMADGLLKSLIIDAVLAG
                     VGGVLVFLPQILILFFFILSLEESGYLPRAAFLLDRLMFKAGLTGRAFIPLLSSFACA
                     VPGIMATRSISD"
     gene            complement(1036..1278)
                     /locus_tag="PLY05_13110"
     CDS             complement(1036..1278)
                     /locus_tag="PLY05_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNC04278.1"
                     /translation="MRLSQLAKNTPAIVLSVQKHDDVDPIAKRLSELGFVSGETIRLI
                     TRAPFGGDPILVQVGFTRFALRVSEAERVLVKEVRT"
BASE COUNT          445 a          304 c          222 g          330 t
ORIGIN      
        1 tatcactaat acttcgcgtc gccataatac caggtacagc acaagcaaaa ctggacaata
       61 aaggaataaa tgctcgacct gttaagcccg ctttaaacat taaacgatct aataaaaaag
      121 ccgcacgagg caaatacccc gattcttcta gtgataaaat aaaaaagaac aaaattaaaa
      181 tttgcggcaa aaaaactaaa acaccgccaa cacctgccaa aacagcatca ataattaggc
      241 tttttaataa gccatccgcc atatactggc caattagctc gctaagccat gtaacacttg
      301 tttcaatacc atccataaac ggtgctgccc atgcaaacac tgcttggaaa ataaaaaaca
      361 ttaaaacacc caaaatagct aaaccagcaa cagggtgtaa caaaatacta tctaaaaaat
      421 catcccgttg gtcagtatct ctgggcatga ccaccgtttc agccaataaa tcgcgtactt
      481 gttgatgaat atcgtgatca gggattaatg ttggcttagc taaggcagga atttgttgat
      541 ctaaacttgc caataattga tcaataccat gacgtttaac agcggctgtt tcaataactt
      601 taatgcctaa gcgttgcgat aatattgcac tatctatttg aatcccacga cgtttggcat
      661 catccatcat gtttaatact aacaacattg gacgaccaag tgcttgaact tctaatacaa
      721 aacgcaaatg caaacgcaag ttagttgcat caaccacaca taccaataaa tcaggggtag
      781 gctcacctgc aaattcaccc aaacaaatcg cacgggtaat tgcttcatca gggctagttg
      841 cttctaaact ataagcacca ggtaaatcta aaattcgtaa acgacgacca gatgctaagg
      901 ttaaaaatcc ttctttacgc tcaacagtta ccccagcata gttggcaact ttttgccgcg
      961 ctccagttaa ttgattaaac aaagaggttt tgccgcagtt aggattgcca actagcgcaa
     1021 tctgttgaat tgcagtcatg tgcgtacctc tttgactaag acacgctccg cctcactgac
     1081 tctcaatgca aaacgagtaa atcctacttg tactaaaatg ggatctcccc caaatggcgc
     1141 acgagtaatt aggcgaattg tttcaccact gacaaaacct aattcgctta aacgtttggc
     1201 aatcggatca acatcatcgt gtttttgtac cgataacaca atcgcaggcg tgtttttggc
     1261 taactgagaa aggcgcacta tgctatggcc tcaacataaa a
//
LOCUS       DAOQMB010000677         1301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89223_length_1301_cov_8.416533, whole genome shotgun sequence.
ACCESSION   DAOQMB010000677 DAOQMB010000000
VERSION     DAOQMB010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1301
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89223_length_1301_cov_8.416533"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..266
                     /locus_tag="PLY05_13115"
     CDS             24..266
                     /locus_tag="PLY05_13115"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HNC04279.1"
                     /translation="MRLSQLAKNTPAIVLSVQKHDDVDPIAKRLSELGFVSGETIRLI
                     TRAPFGGDPILVQVGFTRFALRVSEAERILVQEVRV"
     gene            263..>1301
                     /locus_tag="PLY05_13120"
     CDS             263..>1301
                     /locus_tag="PLY05_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNC04280.1"
                     /translation="MTTIQHIALVGNPNCGKTSLFNQLTGARQKVANYAGVTVERKEG
                     FVTLASGRRLRILDLPGAYSLEATSPDEAITRAICLGEFADETSPDVLVCVVDATNLR
                     LHLRFVLEVQALGRPMLLVLNMMDDAKRRGIEINTTKLAALLGIKVIETAAVKRQGID
                     ELLANLDQQIPALAKPTLIPDHDIHQQVRSLLVETVVMPRDTDQRDDFLDSILLHPIA
                     GLAILGVLMFCIFQAVFAWAAPFMDGIEAGITGLSELIGQYMADGLLKGLLIDAVLAG
                     VGGVLVFLPQILILFFFILVLEESGYLPRAAFLLDRLMFKAGLTGRAFIPLLSSFACA
                     VPGIMATRSISD"
BASE COUNT          344 a          221 c          307 g          429 t
ORIGIN      
        1 ttttatgttg aggccatagc atagtgcgcc tttctcagtt agccaaaaac acgccagcaa
       61 ttgtgttgtc ggtacaaaaa catgatgatg ttgatccgat tgccaagcgt ttaagcgaat
      121 taggttttgt cagtggtgaa acaatccgct taattactcg tgcgcctttt ggcggtgatc
      181 caattttagt tcaggtgggg tttactcgtt ttgctttacg agtcagtgag gcggagcgga
      241 ttttagtcca agaggtgcga gtatgaccac tattcaacat attgcacttg ttggtaatcc
      301 taattgtggt aaaacctcat tatttaatca actaacaggt gcacggcaaa aagttgctaa
      361 ctatgcaggg gtcaccgttg aacgcaaaga aggatttgta actttagctt caggccgaag
      421 attacgtatc ttagatttac ctggcgcata tagtttagaa gccactagtc ctgacgaagc
      481 gattacccga gcgatttgtt tgggcgagtt tgccgacgaa acctcgcccg atgtcttagt
      541 gtgtgtggtg gatgcgacaa acttacgttt gcatttgcgt tttgtgttag aagtacaagc
      601 cttaggtcgt cccatgttat tagttttaaa catgatggat gatgctaaac gccgtggtat
      661 tgagattaat actaccaaac tagcagcctt attagggatt aaagtgattg aaacggcggc
      721 tgttaaacgt caaggcattg atgaattatt agcaaattta gatcaacaaa ttcctgcctt
      781 agctaagcca acattaatcc ctgatcacga tattcatcaa caagtacgca gcttgttggt
      841 tgaaacagtg gtcatgccta gagataccga tcaacgtgat gactttttag acagtatttt
      901 attacatccg attgcaggat tggccatttt aggcgtgtta atgttttgta tttttcaggc
      961 agtgtttgca tgggcagcac cgtttatgga tggtattgaa gcagggatta cagggttaag
     1021 tgagcttata ggccaatata tggctgatgg tttattaaaa ggcttattaa ttgatgcggt
     1081 gttggctggg gttggcggtg ttttagtttt tttaccacaa attttaattt tattcttttt
     1141 tattttagta ttagaagagt caggttattt accacgagca gcgtttttat tagacagatt
     1201 aatgtttaaa gcaggtttaa caggcagagc atttattccc ttattatcca gttttgcttg
     1261 tgctgtacct ggtattatgg cgacgcgaag tattagtgat a
//
LOCUS       DAOQMB010000678         1300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89351_length_1300_cov_31.476305, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000678 DAOQMB010000000
VERSION     DAOQMB010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89351_length_1300_cov_31.476305"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(966..1130)
                     /locus_tag="PLY05_13125"
     CDS             complement(966..1130)
                     /locus_tag="PLY05_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04281.1"
                     /translation="MKYSKFYAVLSQKANLVLWLIFITVFSLGLGTLILGGEFAMQLE
                     PFKIEVKQQK"
BASE COUNT          435 a          258 c          232 g          375 t
ORIGIN      
        1 cacagcaaca aacaacactc gacaaggcag aagcacgact aaaaggtgca aaacaagctc
       61 gttttaaggc cgctaaaggt ggaagcatga agcagttaat tactgagttt agtctcgaca
      121 tcaacgaagc catgatgaaa aatattcaat agcctaaata agtaaaactg attatcctta
      181 cctaattagt aaaggttttt agatacaaaa cagcccatgc cttgggaatg ggctgttttt
      241 attatcatca atcggctata actgataaca acattggata atttaatcta gaatcaggca
      301 cgatgaaagt tagattttat aaagttactg tgattgtaaa tggtatagaa accgtcttgt
      361 acatcccagc ttcagggaaa aataatctta ctcgaatatt gaaggcattc gagctatcta
      421 cagtcaaaat cgaattttta gggtggcata tagttaatat tttttgcagc cctaaagatg
      481 gtgtctattt ttctgcaaat attgatgaaa aacccatttt ttatcagaaa aatcaattag
      541 gctttgaata cttattcaac tgcttggaca gccataaata taagaaagtt tcatataatt
      601 caactgttta acctgaacca actagcacaa ataacgtaac tgaattatac tgcctccaga
      661 atatttcctt tccttgtttc aacggattcg atgatggact gtcaatccaa tcctcaaacc
      721 ctagggtttg aggattggat caacgaaagt tgattcatca ttgtgtttgt actgcttcta
      781 acagtacgaa ctatattctg caaaaagctc atcggctaag gatgggcttt ttgtttttca
      841 ggcataaaaa aagcccttca aatgaagggc tttataaaag atcagtgcga agctgatcca
      901 atttagcaaa agaaaatcta catcttgaag catcagtggg actggccgaa gccagcccct
      961 atgccttatt tctgttgttt cacttcaatt ttgaagggtt caagctgcat agcaaactcg
     1021 ccccctaata tcaaagttcc caaacccaaa ctaaataccg tgatgaaaat caaccacagc
     1081 acaaggtttg ctttttgaga cagaacagca taaaatttac tgtacttcat ctagtaaatt
     1141 tcctgatgtt gtacggtcgc aaaacaaccc ccaaccatac ggcagggcgc gttggccaat
     1201 ccaacatcct gactattgca agtagtcagt acggacattc taaacaaaaa agccactgtt
     1261 taataccgtg gcttttttgt ttctagagct tgtgcaaggc
//
LOCUS       DAOQMB010000679         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89482_length_1299_cov_6.842444, whole genome shotgun sequence.
ACCESSION   DAOQMB010000679 DAOQMB010000000
VERSION     DAOQMB010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89482_length_1299_cov_6.842444"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(152..231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            433..>1299
                     /locus_tag="PLY05_13130"
     CDS             433..>1299
                     /locus_tag="PLY05_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002059161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HNC04282.1"
                     /translation="MSINHFDVIIIGGGASGLMCAASAGQRGRRVLVLDSSNKVGKKI
                     LMSGGGRCNFTNLFVEPSNYLSANPHFCISALNRYTQWDFIALVEKHRIPYHERKHGQ
                     LFCNDSAKDILAMLLAECERTQQVTIQTHCQIEKIIPVDDGEHRFHLQTSKGQFACQS
                     LVIATGGLSIPTMGASGFGYEVAKQFGHQVLPTRAGLVPFTFTDNIKAVTERLSGLAL
                     DVSMTAKDSTSFHENLLFTHRGLSGPVALQLSNYWQLGETVEVNLLPQLEMASWLKEQ
                     KQQHPKSLLRTQL"
BASE COUNT          376 a          235 c          299 g          389 t
ORIGIN      
        1 tttatagtcg ccaacggatg tcattgcagt catgacagac tcctatggta tagagcttac
       61 gcgttgcatc gcaattatcg tgttttgtga atatgaaatc gttgaaaacg attgaaatgt
      121 gaacgaataa gcgataacag cgtaagtgct aagtgagtgg gtgacgttgg caactatatc
      181 tttactaccg agcctagcag tgttttaact ggtgcagcac ccctcggcag tcggagagca
      241 ttttaacgga taatcagcaa aaaagaaaag tgaacaaatt gggacttacg cggttatcgc
      301 taatttattc gcttaataat cgttttaaac gattattagc tcacaaaacg cgctaattgc
      361 agcacaatgc gtaagtccat aattaaaaaa tgctaaaatc acgctctttt gttaaatagt
      421 tgtataaggt ttatgtcgat taatcatttt gacgtaatta ttataggtgg cggtgcctcg
      481 ggattaatgt gtgcagccag tgcaggacag cgtggccgtc gcgtgttggt gttagatagt
      541 agcaataaag tcggtaaaaa gattttaatg tcaggcggtg gccgttgtaa ttttaccaat
      601 ttatttgttg agcctagtaa ttatttatct gcaaacccac atttttgtat ttctgcgtta
      661 aaccgatata cccagtggga ttttattgcg ctggttgaaa agcatcgtat tccttatcac
      721 gaacgtaaac atggccagct gttttgtaat gattcagcta aagatatttt ggccatgttg
      781 ttggcagagt gcgaacgtac tcaacaagta accattcaaa cccattgtca aattgaaaaa
      841 attatcccag tcgatgatgg cgagcatcgt tttcatttgc agaccagtaa aggtcagttt
      901 gcctgtcagt ctttagtgat tgccacaggt ggtttgtcta tcccaacgat gggtgcgagt
      961 ggttttggtt atgaagtggc taaacagttt ggccatcagg tgttgccaac acgcgcaggt
     1021 ttagtgccat ttacctttac tgataacatc aaagcagtca ccgaacgatt atcgggtttg
     1081 gcgttggatg tcagtatgac agcgaaggat agcaccagtt ttcatgaaaa cttgttgttt
     1141 acccatcggg gcttaagtgg gccagtcgct ttgcaattat caaactattg gcaattaggc
     1201 gagacggtag aagtaaattt attgccacag ctagaaatgg cgagttggct aaaagagcaa
     1261 aagcagcaac atcctaaatc attattacgt acacagtta
//
LOCUS       DAOQMB010000680         1299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89513_length_1299_cov_3.569936, whole genome shotgun sequence.
ACCESSION   DAOQMB010000680 DAOQMB010000000
VERSION     DAOQMB010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89513_length_1299_cov_3.569936"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="PLY05_13135"
     CDS             <1..616
                     /locus_tag="PLY05_13135"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HNC04283.1"
                     /translation="HLAHYHVYADARCILTSRDKHSGKQYRGGLLSYLKAKNDIQTWL
                     KEDGRTSCRFSTMFDFYALPNDFPDFTNAQKKTDPYQKIACLEAALAQDILDNRFIPY
                     IQLHEFEALILADAQKLDWEYLEHERAIANLVAMVGDTCPELINDGAETAPSKRILKQ
                     IPEYDKVTAGWTVAEQIGLTTLRHKCPHFNEWLTRLEQLGATGE"
     gene            609..>1299
                     /locus_tag="PLY05_13140"
     CDS             609..>1299
                     /locus_tag="PLY05_13140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HNC04284.1"
                     /translation="MSNSKIQQALQNLFEKHRIVFWYDAKQEFVGDFEQLTLANVEKI
                     QLNNNEFGVKYRVLRQEPEHKFLLYAAYAQPEDLENWLLDIQLAQGEFRTDQVALWLA
                     ELELGLGFADVIKDHSEFFQASKRKDNLKRLLVADDTPHLVRLKMLAICSSADSGLDT
                     ILESLLAELADDRDDKMRLIQRCSLDGFLWQQLQRHYGYESTNPSIKDFSLELFKSCY
                     ALATAQTAKLSH"
BASE COUNT          397 a          266 c          271 g          365 t
ORIGIN      
        1 gcacttggcg cattaccatg tgtatgccga tgcgcgttgt attttaacta gcagagataa
       61 acactcaggc aaacaatatc gaggcggttt acttagctat ctaaaagcca aaaacgatat
      121 tcagacatgg ttaaaagagg atggtcgtac aagctgtcgt tttagcacca tgtttgattt
      181 ttatgctttg cccaatgatt ttccagactt tactaacgct caaaaaaaga ctgaccctta
      241 tcaaaagatt gcttgtttag aagctgcttt agcacaagat attctagaca atcgttttat
      301 cccctatatt caattacatg aatttgaagc cttgattttg gcagatgctc aaaaactcga
      361 ttgggaatat ttggagcatg agcgagcgat tgccaattta gttgcaatgg ttggcgatac
      421 ttgtcctgaa ctgattaatg acggtgcaga aaccgcccca tctaaacgta ttcttaaaca
      481 gattcccgaa tacgacaaag tgaccgcagg atggacagtg gctgaacaaa ttggcttaac
      541 gacattacgg cacaaatgcc ctcattttaa tgaatggcta acacgtttag agcaattagg
      601 agcaacgggt gagtaacagc aaaatccaac aggcactaca aaatctattt gaaaagcatc
      661 gtattgtttt ttggtacgat gccaaacaag agtttgtggg tgactttgaa caacttacct
      721 tagcaaatgt tgaaaaaatt cagcttaata ataatgagtt tggcgttaaa tatcgggttt
      781 tacgccaaga gcctgaacat aaatttttac tatatgcggc ctatgctcaa cccgaagact
      841 tagaaaattg gttattagat attcaactgg cacagggcga atttagaacc gaccaagtgg
      901 ctttgtggct ggccgaatta gaacttggtt taggttttgc cgatgttatc aaagaccata
      961 gtgagttttt tcaagccagt aaacgtaaag acaacctcaa acggctatta gttgcggacg
     1021 acacgcctca ccttgtccgc ctaaaaatgt tggctatttg cagctctgct gacagtggcc
     1081 ttgataccat tttagaaagc ctactggcgg agctggctga cgaccgtgat gacaaaatgc
     1141 gtcttattca gcgttgcagc ctagacggtt ttctatggca acaactacaa cggcattatg
     1201 gttatgaatc tactaacccc agtatcaaag actttagtct agaactgttt aagtcgtgtt
     1261 atgcccttgc aaccgcacaa acagccaaac tcagccatc
//
LOCUS       DAOQMB010000681         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89629_length_1298_cov_17.301689, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000681 DAOQMB010000000
VERSION     DAOQMB010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89629_length_1298_cov_17.301689"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1298
                     /locus_tag="PLY05_13145"
     CDS             <1..>1298
                     /locus_tag="PLY05_13145"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HNC04285.1"
                     /translation="QIAAHNLTAANRVIPDLTIVAEGTRENHQITWQMLAEPVVAEGI
                     LQGAIDNQGNWLGQNTNGTVKIDDFLWQQQAPFVSQWQMADKKLSLAAHCWQAEQAQL
                     CNQDTLVLSPQLVSANLTLQGLEINRLQHLLPEGLAWRGDLQGQLNFAWQAQHKPQFN
                     LQVLTQNGALGLTQEDEEPLTLPYHQLKLSAKDDENNQVGLRFDMQAPNMGQGYIDAR
                     IDPQNQPYQINGAMLLEKVNLAMFKPFFPAIRHLSGEMNLAGGLTGEITKPDFYGEFS
                     LNDGEILAKNAPLDFSHTNIKASIRGKQATINGQLNSGKGLAQLKGDVSWQDTPQFNL
                     NIDGKRLEFAQKPLFKAKVSPNLHLQIKPYVINLKGDALVEDALLNPQNLSDQAVPLS
                     ADVRVVDLSAPDRIRVAKAMNQWDINADINLRMGDNVVFNG"
BASE COUNT          417 a          247 c          265 g          369 t
ORIGIN      
        1 caaattgctg cacataattt gactgcggct aatcgagtca ttcccgattt gaccatcgtt
       61 gctgaaggta cacgcgaaaa tcatcaaatt acatggcaaa tgctggcaga acctgttgta
      121 gcagagggaa ttttgcaagg tgctattgat aatcagggaa attggctggg gcaaaatact
      181 aatggtacgg tcaaaataga tgatttttta tggcaacaac aggctccctt tgtcagtcaa
      241 tggcagatgg ccgacaaaaa actcagccta gccgcacatt gttggcaagc agagcaagcc
      301 caactgtgta atcaagatac cttagtgttg agtcctcaat tggtatccgc caacctcaca
      361 ttacagggat tagaaattaa tcgtttacaa catctattgc ccgaaggttt agcatggcgc
      421 ggtgatttgc aaggccaact aaattttgct tggcaagcac agcacaaacc tcaatttaat
      481 ttgcaggtat taacccaaaa tggtgcattg ggtttaactc aagaagatga agaaccactg
      541 acattgcctt atcatcaatt aaaactaagt gccaaagacg acgaaaataa tcaggttggt
      601 ttacgatttg atatgcaagc gcccaatatg ggacaaggat atattgatgc gcgtattgac
      661 ccacaaaacc aaccctatca aatcaatggt gcaatgttat tagaaaaagt taatttggcc
      721 atgtttaagc cattttttcc tgctattcgc cacttatcag gtgaaatgaa tttagcaggt
      781 ggtttaacag gtgaaattac taaacctgat ttttatggtg aatttagttt aaatgatggt
      841 gaaatattag ccaaaaatgc ccccttagat tttagccata ccaatattaa agccagtatt
      901 cgtggtaaac aagcaacgat taatggtcaa ctgaacagtg gtaaaggttt ggcacaactc
      961 aaaggtgatg tatcttggca agatacacca cagtttaatc ttaatattga tggcaaacgt
     1021 ttagagtttg cccaaaagcc cttatttaaa gccaaagtta gccctaattt acatttacaa
     1081 atcaagcctt atgttattaa tttaaaaggt gatgccttag ttgaagatgc cttattaaat
     1141 ccgcaaaact tatccgacca agcagtgccg ttatctgctg atgtacgtgt ggtcgattta
     1201 tccgcgcctg atcgtattag agtcgctaaa gccatgaatc aatgggatat taatgccgat
     1261 attaacttac gcatgggtga caatgtcgta tttaatgg
//
LOCUS       DAOQMB010000682         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89630_length_1298_cov_14.403057, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000682 DAOQMB010000000
VERSION     DAOQMB010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89630_length_1298_cov_14.403057"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            358..1107
                     /locus_tag="PLY05_13150"
     CDS             358..1107
                     /locus_tag="PLY05_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016656408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNC04286.1"
                     /translation="MAGHSKWANIKHRKARQDASKGKVFTKYLREIVVAAQMGGGNEA
                     DNPRLRAIVSKALSANMTRDVINRAIQRGVGGGDADKVQEITYEGYGVGGVAVLVETM
                     TDNLKRTVSDVRHAFTKCGGNLGTSGSVDYLFSKRGELTFAEGDEDKIMDIALEAGAD
                     DVISEADGTVMVITTPHNFGHIQDALEAAGLKADHAEVTMHPSTQADITDLEQAQKIM
                     KMIDMLEDLDDVQNVYSNVHFSDEVMDALNK"
BASE COUNT          405 a          249 c          253 g          391 t
ORIGIN      
        1 tgctcaaagg ggcgcgtgtc accttgccct gcaagcacat tacccactac aggcacttcg
       61 tacaccgaag aaatacagcc acttaaactt aatatggcta acaaacaagc aatgtaacgc
      121 atctttttat ccttttactt acgtttaaac gtttttacta aaacacaaaa catgccatat
      181 cattaaaatc aataatttca aatagttaaa actattaaag tctagtatta ctggtttaaa
      241 taaacataaa ttgttaaatc aggggcattt tttgtaggta ttaacacaag gcaaaacttt
      301 tctcatattg tataatttag accatcattc tatatcatta ttttttagga catttttatg
      361 gctggccatt ctaaatgggc gaacattaaa catcgtaaag ctcgccaaga tgcctccaaa
      421 ggcaaagttt ttaccaaata tttacgcgaa attgttgtag ctgcccaaat gggtggtggc
      481 aatgaagcag ataatccccg tttacgcgcc attgtttcta aagccttatc ggccaatatg
      541 acccgcgatg tcatcaaccg agccattcaa cgtggtgttg gtggtggaga tgccgacaaa
      601 gttcaagaaa ttacctatga aggttatggt gtcggtggtg ttgcggtgtt agtcgaaacc
      661 atgactgata acttaaagcg tactgttagt gatgtccgtc atgcctttac taaatgtggt
      721 ggcaatttag gcacatcagg ctctgtcgat tatttattta gtaaacgtgg cgaattaact
      781 tttgctgagg gtgacgaaga taaaatcatg gacattgccc tcgaagcagg tgctgacgat
      841 gtgattagtg aagccgatgg cacagtgatg gttattacca ctccgcataa ctttggtcac
      901 attcaagatg ctttagaagc ggctggttta aaagctgacc atgccgaagt gaccatgcac
      961 ccttctactc aagctgatat taccgacctt gaacaagcgc aaaaaattat gaaaatgatt
     1021 gatatgcttg aagatttaga cgatgtgcaa aacgtttaca gtaatgtgca tttcagtgat
     1081 gaggtcatgg acgcgttgaa taaataagat tatttgctta ataaaagctg ttgaggtttt
     1141 gtttatgttg acaaacaaaa cctcaacggt ctctaaaaat aatttaaaaa atctccattt
     1201 tctgtcacaa actgcacagc tccttcgttt tagttatagt tttatcgagg ataccactat
     1261 gactaatacc acacagccaa ctgtcatcat tttagggg
//
LOCUS       DAOQMB010000683         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89631_length_1298_cov_11.640386, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000683 DAOQMB010000000
VERSION     DAOQMB010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89631_length_1298_cov_11.640386"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLY05_13155"
     CDS             <1..196
                     /locus_tag="PLY05_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018678890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04287.1"
                     /translation="TEALKPRKVVNMVRIDAKHNEKNKVAAAYNHAQHLPYRRQMMQD
                     WANYLDNVRTGKVLQFVKAS"
     gene            386..646
                     /locus_tag="PLY05_13160"
     CDS             386..646
                     /locus_tag="PLY05_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HNC04288.1"
                     /translation="MQDFKRKHANARKALDAWQIEVAHSHWQTSHDIKQRYPSADFLA
                     DNRVIFNIKGNHYRLVVKVRYQRGLVVIEWVGTHAEYDKQSF"
     gene            655..1062
                     /locus_tag="PLY05_13165"
     CDS             655..1062
                     /locus_tag="PLY05_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HNC04289.1"
                     /translation="MQVKVIKHRTDYDVAMKRLNELLDIDPQEGTELADELELLLVVI
                     KAYEKAIVPPVVVDPIEAILFRMEQMGLTRKDLEPYLGSVSKVSEVLNRKRPLSMTMI
                     RKLHRGLGISADILIEETPKYIPSKDYSLSMGV"
BASE COUNT          383 a          227 c          305 g          383 t
ORIGIN      
        1 aaccgaagcc ttaaaaccgc gcaaagtggt aaatatggtg cgtattgatg ccaaacataa
       61 tgaaaaaaac aaagtagcag ccgcttataa ccatgctcaa catttaccgt ataggcggca
      121 aatgatgcaa gactgggcaa attatttaga taacgtgcgg acaggtaaag tgctgcaatt
      181 tgtgaaagcc agttaaaacc ttgtctaagc cttgccatat aaaccgccaa caaaaggcgg
      241 ttttttattg tgttgtgttt tgtcgcggac tatcacggtt tgtcttgata cgatttctag
      301 ttgatgtatt ccctatttgg gaatacacta aaagtcgttt aataaatagg caaggcatga
      361 cgtcgaatta ttaggcttac cccttttgca agactttaag cgcaaacacg ctaatgcacg
      421 aaaagcctta gacgcttggc agattgaagt agcgcatagt cactggcaaa catcacatga
      481 tattaaacag cgttacccca gtgctgattt tttagcggat aaccgtgtta ttttcaatat
      541 caaaggtaat cattatcgct tggtggttaa ggttcgttat caacgtggat tagtggttat
      601 agagtgggtg ggtactcatg cagaatatga caaacaaagt ttttgaggtg catcatgcaa
      661 gttaaagtca ttaaacaccg tactgattat gatgtggcca tgaaaaggct taatgagttg
      721 ctagatattg acccacaaga gggtacagag ttagccgatg aattagagct gcttttagtg
      781 gttattaaag cctacgaaaa agcaatcgtg ccgcctgtgg ttgttgaccc catagaggcg
      841 attttattca gaatggagca aatgggctta acaagaaaag atttagagcc gtacttaggt
      901 tctgtttcta aggtgtcaga agtgcttaac cgtaaacgcc cattatccat gacgatgatt
      961 agaaaattgc atcgtggttt gggtatttcg gcagatattt tgattgagga aacgcctaaa
     1021 tatataccta gcaaagatta ttctttgtct atgggtgttt aggtgtttta acctgtcgtg
     1081 ttctatcacg gcctatattg tacggtggcg gtgtagtgct taaaatgggt gtgctaggta
     1141 tagggcggcc acccgacaag taagtaatcc ctaaacgctt atttacctag ccctagtttg
     1201 gtttagggga agctttaggg ggcgcgtgat gactaatgaa tctatggcta atcatatgaa
     1261 aacgtcattg tgtgtcagtg cattacaaat ggcctttt
//
LOCUS       DAOQMB010000684         1298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89632_length_1298_cov_9.917940, whole genome shotgun sequence.
ACCESSION   DAOQMB010000684 DAOQMB010000000
VERSION     DAOQMB010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89632_length_1298_cov_9.917940"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..349)
                     /locus_tag="PLY05_13170"
     CDS             complement(56..349)
                     /locus_tag="PLY05_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04290.1"
                     /translation="MLKNLIEGRSTKVYTLAAMLAVMWLFSWWTDDGQVLKSYQTQGV
                     IEQIYEKAYLIRLADGRSVRVMREGEYVKGASVKLQITQYESKKERAKLLPKS"
     gene            complement(330..995)
                     /locus_tag="PLY05_13175"
     CDS             complement(330..995)
                     /locus_tag="PLY05_13175"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNC04291.1"
                     /translation="MRVLSFVILSLLMQVSYAQPEWELSKEDTQRNIQVFTSSQEDSS
                     YHQFKAVTLVPQNPQTVLAVLSDISAWPQWLARIKQVKVLKKEKDRSWVYVVYKLPYP
                     FIERDMVVYNQVQSLANGQIIIKTQALNNYPMAVKNDVKRVRLTNFNSTWQLTPLAKS
                     GTKVEFWGSGDPEGFVPALIFNYNLADEPLQSLRQLRKMLLRDKYQKNNTQTHDKNAQ
                     KFD"
     gene            complement(998..>1298)
                     /locus_tag="PLY05_13180"
     CDS             complement(998..>1298)
                     /locus_tag="PLY05_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04292.1"
                     /translation="NQLLSPEQITKYTQQYADVDMWQLTGQDEDYFPFVIGEQITNYY
                     IVYWSKKHGIIKYYDTDLVRYRAFLLWLLDNMHPVFANQQQAEDFAKAREWPRVD"
BASE COUNT          374 a          255 c          242 g          427 t
ORIGIN      
        1 acagttttat ttgttgattt aaatttttat taatattgat tgttatagtt tgattctaag
       61 attttggcaa caatttagcg cgttcttttt tagattcgta ttgggtaatt tgtaatttga
      121 cagatgcacc cttgacatat tcgccctcac gcatcacacg caccgagcgg ccatcagcaa
      181 gcctaattaa ataagctttt tcgtagattt gttcaatcac accttgggtt tggtaagact
      241 taagcacttg gccatcatct gtccaccaag aaaacaacca catgacggcg agcatagcag
      301 ctagggtata aactttggtg gagcgtcctt caatcaaatt tttgagcatt tttatcgtga
      361 gtctgggtat tatttttttg atatttatca cgcaagagca ttttgcgcaa ctgacgtaaa
      421 ctttgtaagg gttcatcggc taggttgtag ttaaaaatca gcgcaggaac aaatccttct
      481 ggatcaccac tgccccaaaa ttcaacttta gtgcctgatt ttgctaacgg tgttagttgc
      541 catgtgctat taaaattagt taaacgcacc cgttttacat catttttaac agccattgga
      601 taattgttta aggcttgtgt tttgatgatt atttgaccat tcgccaatga ctgcacttga
      661 ttgtaaacca ccatatcacg ctcaataaaa ggataaggca atttataaac cacatagacc
      721 caactacggt ctttttcttt ttttaagact ttgacttgtt taatgcgtgc taaccattgt
      781 ggccatgcag aaatatccga caaaactgct aataccgttt gtggattttg tgggacgagc
      841 gttacggctt taaattggtg atatgagcta tcttcttgag agctggtaaa tacttgaata
      901 ttacgctgtg tgtcttcttt gctaagctcc cactcaggct gagcataact gacctgcatc
      961 agtaaagata aaatgacaaa actcagcacg cgcataatta atccacacgc ggccactccc
     1021 gcgcttttgc aaaatcttcg gcttgttgtt ggttggcaaa tacagggtgc atattgtcta
     1081 ataaccacaa caaaaaggca cgatacctca ccaaatcagt atcatagtat ttgataatac
     1141 catgcttttt tgaccagtaa acaatgtagt agttagttat ctgctcacca atgacaaaag
     1201 gaaaataatc ttcatcttga cccgttaatt gccacatatc aacatcggca tattgttgag
     1261 tatatttggt aatttgttca gggcttaaga gttgatta
//
LOCUS       DAOQMB010000685         1297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_89808_length_1297_cov_3.875201, whole genome shotgun sequence.
ACCESSION   DAOQMB010000685 DAOQMB010000000
VERSION     DAOQMB010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89808_length_1297_cov_3.875201"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            426..677
                     /locus_tag="PLY05_13185"
     CDS             426..677
                     /locus_tag="PLY05_13185"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNC04293.1"
                     /translation="MMMTQVRKIGNSLGHIIPMALAQQFGLVEGTKLHIKEHNGTIII
                     EPVVTKKFPFSEQELLNDLNGYAAHADELASISTTEWQS"
     gene            677..1027
                     /locus_tag="PLY05_13190"
     CDS             677..1027
                     /locus_tag="PLY05_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNC04294.1"
                     /translation="MAAYIPHRNDIVFLDFEPTKGKEIGKYRPALVLSSREYQQKTGL
                     LICCPISTSIRGHITEVAISNMDEPCVVAASIIQTLSWKERQVKKVTTAQPHVINEVL
                     LRLLPLIGADELFN"
     gene            complement(1031..>1297)
                     /locus_tag="PLY05_13195"
     CDS             complement(1031..>1297)
                     /locus_tag="PLY05_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HNC04295.1"
                     /translation="PVVEATRHLPKLHAPLTAISAAIVGVIVNLALFMAYHVFWPQGF
                     GQTADYLALLLSVTAFVALVRFNIGVIPLLAVCATLGFVVKGVL"
BASE COUNT          407 a          278 c          288 g          324 t
ORIGIN      
        1 aacgatagtg acaacgcgtg gattgtctac ttcggcagcg gcagcgtcag ctacggctct
       61 aagaacaata acaactatgt gcggttagtt cgttccagtc agtaaattgg cagcgtagcg
      121 gttttggctt gggggtgctt gggatgctct tgtcatgcct aagaatgagg catctttgag
      181 attcctcacg ttgctcggaa tgacaaaaaa tcctccccag cccctcttta caaaagaggg
      241 ggacagggca gtgcaagcat acaaagcgtc tccccctttg ccaaaggggg attaaggggg
      301 atttttggga atccacccca gccctccttt accaaaggag gggcatcatc gctaataagg
      361 gacgttgact tataacaaag ttgacataca ataagttata acattgttat aatttttgag
      421 gtgcaatgat gatgacccaa gtcagaaaaa ttggcaatag tttaggtcac ataatcccca
      481 tggcattggc gcaacaattt ggcttggtcg aagggacaaa gctgcatatt aaagaacaca
      541 atggcacaat catcattgaa cctgtggtca ccaaaaaatt ccccttttct gagcaagagc
      601 tactcaacga tttaaatggc tatgcggcac acgctgacga attggcaagc atttctacaa
      661 ctgaatggca atcgtaatgg ctgcttatat tcctcatcga aatgacatag tttttttaga
      721 ttttgaaccc acaaaaggca aagaaatcgg caaatatcgc cctgcattgg tgctgtctag
      781 ccgagaatat caacaaaaaa caggtttgct tatttgttgt cccatcagta ccagtatcag
      841 agggcatata acagaagtgg caataagcaa tatggacgaa ccgtgtgtgg ttgcggcaag
      901 tattattcaa acattatctt ggaaagagcg acaagtaaaa aaagtcacta ccgctcaacc
      961 acatgttatc aatgaagttt tgttaagatt attaccactt attggagcag atgagttgtt
     1021 taattagcta ctacaacact cctttaacca caaaaccaag tgtggcgcat actgccaata
     1081 agggtatcac gcctatatta aagcgcacta aagcgacaaa tgccgttacc gatagtaaca
     1141 aagccagata atcggctgtt tggccaaagc cttgtggcca aaatacatga taggccataa
     1201 ataacgccaa attcacaatc acaccgacaa tagcggcact aatggcggtt aaaggcgcgt
     1261 gaagtttagg taaatgacgc gtggcctcta ccactgg
//
LOCUS       DAOQMB010000686         1295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_90076_length_1295_cov_5.852419, whole genome shotgun sequence.
ACCESSION   DAOQMB010000686 DAOQMB010000000
VERSION     DAOQMB010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90076_length_1295_cov_5.852419"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="PLY05_13200"
     CDS             <1..449
                     /locus_tag="PLY05_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC04296.1"
                     /translation="NLMVILIPFLLLDAVFTQQAMLQVLMPSGEGAAAETPKPPLVLE
                     VMVYKDKFLVADRQTGPMKTITTNDKGNLDYKALNDYLRTVKTQYPLVTDASILLEQD
                     TPYDKLIHTMDAVRIVVSTETGREARYALFPDISIGDAPPDIGGAK"
     gene            446..946
                     /locus_tag="PLY05_13205"
     CDS             446..946
                     /locus_tag="PLY05_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNC04297.1"
                     /translation="MKKSPRVKRHERLQRRIKGQANLNLTSLMDIFTILVFFLLVSSS
                     NPSKLPDAEKIKLPVSIAQTEPNETLTVMITKDDVLVQGLKIGTINEIATLKTDLIPN
                     LATELKYRASHTVAPLNERGIPERDILILGDEKTPYSVIRKVMATCSDNDYAKISFAV
                     TRGGKQ"
     gene            946..>1295
                     /locus_tag="PLY05_13210"
     CDS             946..>1295
                     /locus_tag="PLY05_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04298.1"
                     /translation="MSTHLISGFLPWDELPEHSNRARKINAIALVLAFLCFVVIPFIK
                     VPPVPRSEVKVPERVARAIERKIELPPPPPKPPEVKKEEKKEVKAEDKPKPKEEIKPK
                     PIEKEPVAKPKATE"
BASE COUNT          425 a          259 c          249 g          362 t
ORIGIN      
        1 tgaatttgat ggtgattcta atcccattct tgttgctaga tgcagtgttt actcaacagg
       61 ccatgctaca ggtattaatg cctagtggtg agggtgctgc tgctgaaaca ccgaaaccgc
      121 ctttagtctt agaagttatg gtctataaag ataaattttt agtggcagat cgtcaaacag
      181 gcccaatgaa gactattaca actaacgata aaggcaattt agattacaaa gcattaaatg
      241 actatttgcg tactgtcaaa acacaatacc cattagtcac cgatgccagt attttgcttg
      301 aacaagatac accatatgac aaactcattc acactatgga tgctgttcgc attgtggtaa
      361 gtactgaaac tggtcgtgaa gcacgttatg ctttatttcc agacattagt attggtgatg
      421 caccccctga tattggaggt gctaaatgaa aaaaagtcct cgcgttaaac gccatgaacg
      481 tttacaaaga cgcattaaag gtcaagctaa tcttaactta acctcattaa tggacatttt
      541 tactattttg gttttcttct tattagtgag ttctagtaat ccatctaaac tacctgatgc
      601 tgaaaaaatc aaattacccg tatctattgc tcaaaccgaa ccaaacgaaa ccttaacagt
      661 aatgattact aaagatgacg ttttagttca agggttaaag attggcacga tcaatgaaat
      721 tgctacactt aagacagatt taatccctaa tttagcaaca gagctaaaat accgtgcatc
      781 tcatactgtg gcaccactta atgagcgtgg tattccagaa cgtgacattc tgattttagg
      841 tgatgaaaaa acgccatata gtgtgattcg taaagtcatg gctacctgta gcgataatga
      901 ctatgcgaaa atttcctttg ctgtgacacg tggagggaaa caataatgtc aactcattta
      961 atcagtggtt ttttgccttg ggatgaacta cctgaacata gtaatcgcgc ccgaaaaatt
     1021 aatgccattg cattagtgct tgcattttta tgttttgtgg tgattccatt tattaaagtg
     1081 ccgcctgtac cacgctcgga agtaaaagtc cccgaacgtg tggcgcgtgc cattgaacgt
     1141 aaaattgaac ttcctccccc tcctcccaaa ccacccgaag tgaaaaagga agagaaaaaa
     1201 gaggttaaag ctgaagacaa gccaaagcct aaagaagaaa ttaagccaaa gccaatcgaa
     1261 aaagaacccg ttgctaaacc caaagcaact gaaga
//
LOCUS       DAOQMB010000687         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_91708_length_1283_cov_14.793974, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000687 DAOQMB010000000
VERSION     DAOQMB010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91708_length_1283_cov_14.793974"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLY05_13215"
     CDS             complement(<1..982)
                     /locus_tag="PLY05_13215"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNC04299.1"
                     /translation="MSLDIAQLDVFRVNSLGHEIKVGRLAQANRQVYFQYDEDYLKQG
                     HCLSPFRLKFSQELQINIDKSQPLQGVFADSLPDGWGRLLMDRWLRQQGYLPQQVSLM
                     SRLALVGDRGMGALRYRPVHPIADDSLLHLHEIGQQAQAIFEGHHEDVLPQLVNVASS
                     GGARPKALIYINPDNQQIVSQAQRGYEPWLIKFTSNSLALGHDEGRCEAAYLTMAEKV
                     GITVLKWRLHAHQQQAWLMVKRFDAPSALGRLHMHSAAGLLDANFREPSLDYEDLIKV
                     TTKLCQSVAAAKQQFLRALFNWYACNMDDHSKNWAFLQNDEGQWQISPMFD"
     gene            complement(984..1271)
                     /locus_tag="PLY05_13220"
     CDS             complement(984..1271)
                     /locus_tag="PLY05_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC04300.1"
                     /translation="MDSLLTLNSAYDVQQSLAEAVRIRRKQKKWSRQELAERSGVPAA
                     TIKHFESTGQISLRQFLLLWQLLDSLDTILELTSLKQPLPQNIDDVLKLRT"
BASE COUNT          386 a          283 c          230 g          384 t
ORIGIN      
        1 catcaaacat cggactaatt tgccattgtc cctcatcatt ttgtaaaaat gcccaattct
       61 tactatggtc atccatatta cacgcatacc agttaaataa tgctcttaag aattgctgtt
      121 tagccgcagc cactgattga cagagttttg tcgtcacttt aatcaaatct tcatagtcta
      181 atgacggctc tctaaaattt gcatctaaca atcctgcggc actatgcata tgcaaacgcc
      241 ccaatgctga tggcgcatca aaacgtttaa ccattaacca tgcttgttgt tgatgagcat
      301 gtaaacgcca cttaagaaca gtaataccca ctttttcggc catagttaaa taagcagctt
      361 cacagcgtcc ttcatcatga cctaatgcca aactgtttga ggtaaatttt atcaaccacg
      421 gttcatagcc tcgttgtgcc tgactaacta tctgttgatt atcaggatta atataaatta
      481 gggcttttgg tcgcgctcca ccagagctag caacattaac taactgtggt agaacatcct
      541 cgtgatgtcc ttcaaaaatc gcctgagctt gttggccaat ttcgtgtaaa tgcaataacg
      601 aatcgtcagc aataggatga acaggacgat aacgtaatgc gcccatgcct ctatcaccca
      661 ctaaagccaa acgtgacatt aggctcactt gttgaggtaa ataaccttgt tgacgtagcc
      721 acctatccat caacaaacgt ccccaaccat caggtaatga atcagcaaat acgccctgta
      781 gaggctgtga tttatctata ttaatttgca gttcttggga aaattttaat cgaaatggcg
      841 aaaggcaatg accttgtttt aggtagtctt catcgtattg aaaatacact tgtctgttag
      901 cttgagctaa acgacctact ttgatttcat gaccaaggct attgacacga aacacatcta
      961 gctgagcaat atctaagctc atcttaggtt ctcaacttta aaacatcatc aatattttgc
     1021 ggcaaaggct gtttgagtga tgtcaactca aggatagtgt ctaatgagtc taacagttgc
     1081 cacaataata aaaattgtct taatgaaatt tggcctgttg actcaaaatg tttaatagtt
     1141 gccgcaggca caccactacg ctcagcgagt tcttgtcgtg accatttttt ttgtttgcgg
     1201 cgtatgcgta cagcttcagc caaactttgt tgtacatcat aggctgagtt taaggttaaa
     1261 agactatcca taaaaagata acc
//
LOCUS       DAOQMB010000688         1283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_91715_length_1283_cov_5.751629, whole genome shotgun sequence.
ACCESSION   DAOQMB010000688 DAOQMB010000000
VERSION     DAOQMB010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91715_length_1283_cov_5.751629"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PLY05_13225"
     CDS             complement(<1..809)
                     /locus_tag="PLY05_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004798229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC04301.1"
                     /translation="MSNYVPPYQITTAILNRVAEISERLGRWAATHNNSLSPHLRRDN
                     RIKTIQASLAIENNTLSLEQVTAVLDGKRVLGLPHEIQEVRNAFIAYEKLNDWQADNI
                     NHLLAAHHALMAGLLDDAGQFRSGGVGIYRGKELVHMAPPANRVPVLMQQLLNWLKTT
                     DLHPLIASCIFHYEFEFIHPFADGNGRMGRLWQTLILSRWQPILAYLPVETVIRNQQQ
                     LYYTALQQADKSAEATPFIGFMLDALFTAMVEVAESDTESSEKNSDKSSEK"
     gene            complement(806..>1283)
                     /locus_tag="PLY05_13230"
     CDS             complement(806..>1283)
                     /locus_tag="PLY05_13230"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HNC04302.1"
                     /translation="DNLERYLLKKGDLLMCMTDMKNNVALLGHTALMDIDNEFLLNQR
                     VGMLRSKDASIVNYPFLYILTNSEVFIEDLRRRANSGVQVNLSTSEIKNTKFILPPKN
                     IHQRFNEIVEATFGKLFLLEQQQDSLTEIRDTLLPRLISGQLRINQAQDLINEVGA"
BASE COUNT          328 a          251 c          297 g          407 t
ORIGIN      
        1 atcttctccg aacttttatc cgaatttttc tccgaacttt ctgtgtcgct ttcagccact
       61 tccaccattg ccgtaaacaa ggcatccaac ataaaaccaa taaatggcgt ggcttcggcg
      121 gatttatcgg cttgttgtaa ggcggtgtaa tacagttgtt gttggttgcg tatcacggtt
      181 tctacaggca aataagcgag gatgggttgc caacgactta atatcagcgt ttgccacaat
      241 cgccccatac gtccgttgcc atccgcaaaa ggatgaataa attcaaattc ataatgaaaa
      301 atacaactgg cgattaaggg gtgtaggtca gtcgttttta gccagttaag taattgctgc
      361 atgagtacag gcacacgatt ggctggtggt gccatgtgta ccaattcttt gccacgataa
      421 ataccgacac cacccgacct aaactgtcct gcgtcatcca gtagccctgc catgagtgca
      481 tgatgggcag cgagtaaatg gttgatgttg tcggcttgcc agtcgttgag tttttcgtag
      541 gcgataaagg cgttacgcac ttcttggatt tcgtggggta aacctaatac tcgtttgcca
      601 tcgaggacag cagtgacttg ttctaggctg agtgtgttgt tttcgatggc gagtgaggct
      661 tgaatggttt tgatgcggtt atcacggcgt aaatgcggcg ataggctgtt gttgtgggtt
      721 gctgcccaac ggcctaggcg ttcgctaatc tcggcgacac ggttgagtat ggcggtggtg
      781 atttgatagg ggggaacata attgctcatg cccctacctc gttaattaag tcttgggctt
      841 ggttgatgcg gagttggcca ctgatgaggc gtggtagtaa agtgtcgcgt atttcagtta
      901 aagagtcttg ttgttgctct aatagaaaca attttccaaa ggttgcttca acaatctcat
      961 taaagcgttg atgaatgttt tttggaggta atatgaattt tgtatttttt atttcactgg
     1021 tggatagatt aacttgaact ccactatttg ctctgcgtct taaatcttcg ataaaaactt
     1081 cactgttagt taggatatat aaaaaaggat aattaactat gctagcatct ttacttctta
     1141 gcatgccaac acgctgattt aataaaaatt cattatcaat atccatcaat gctgtatggc
     1201 ctaataacgc aacattattt ttcatatcag tcatacacat cagtaagtca ccttttttta
     1261 aaagatatct ttcaaggttg tct
//
LOCUS       DAOQMB010000689         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_92309_length_1279_cov_8.500817, whole genome shotgun sequence.
ACCESSION   DAOQMB010000689 DAOQMB010000000
VERSION     DAOQMB010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92309_length_1279_cov_8.500817"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1028)
                     /locus_tag="PLY05_13235"
     CDS             complement(72..1028)
                     /locus_tag="PLY05_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04303.1"
                     /translation="MIKIALVVCPQTVISSLAMAQDCFNLANQLSGQTLFQMYKVSVD
                     GLNVHLKDMQLKMDGDLTLAENADLVLIPAIGANVKAVIESNPSLITWLAKHENKHLA
                     SLCSAAFVLAASGVLNGYKATTHWHLADHFRRYFPLVNLHPEQLVTHDGLRFCSGGAQ
                     AGLDLCLYLIQFYQGDWLARQVASMLVMDYGRGAQTRFVPRLPPVQQQDPALARLQRW
                     LEQHYAQALSLEDMAQQLHCSPRTVIRRFKEATQLTPHDYLQRVRISAAESLLVEAHY
                     TIDQIATQVGYEDRTAFAKLFKQITGETPAAYRRRFLAQQPS"
BASE COUNT          411 a          273 c          245 g          350 t
ORIGIN      
        1 aaaaccaaag tcgttaccca agtgcgaatt accgataaag gtttaaaacg tttagcaaaa
       61 atgctgcaaa tttaacttgg ctgttgtgct aaaaaacgcc gccgataagc cgcaggcgtt
      121 tcgcccgtga tttgtttaaa taatttggca aaagccgtac gatcttcata accgacttgt
      181 gtggctattt ggtcaatggt gtagtgggct tccactaata atgactcagc ggcactaatc
      241 cgcacgcgtt gtaaataatc atgtggggtg agttgggtcg cttctttaaa acgacgaatc
      301 accgtgcgtg gcgagcaatg taattgttgt gccatatcct ctaaggacaa agcttgggca
      361 taatgttgct ctagccaacg ttgtaaacgt gcgagggcag ggtcttgttg ttgcacaggc
      421 ggtaatcttg gtacaaagcg cgtttgtgcg cctcgaccat aatccatcac taacatactt
      481 gccacttgtc gtgctaacca atcaccctga taaaactgaa ttaaatacaa acacaaatcc
      541 aaacctgctt gtgcaccacc tgagcaaaat cgcaacccat catgagtcac taattgctca
      601 ggatgcaaat taactaaagg aaaatagcga cgaaaatgat cggctaaatg ccaatgagtt
      661 gttgctttat agccatttaa cacgcccgaa gccgccaaga caaaagccgc actacacaaa
      721 ctggctaagt gtttattttc gtgtttagct aaccatgtta tcaatgatgg attgctttca
      781 atcaccgctt taacatttgc accaattgca ggaattagca ctaaatcggc attttcggcc
      841 aaggtcaaat cgccatccat tttcaattgc atatctttta aatggacatt taaaccatcg
      901 acactgactt tgtacatctg aaataaagtc tgacccgata attgattggc caaattaaaa
      961 caatcttgtg ccattgccaa actagaaatg acggtttgtg ggcaaacaac taaagcaatt
     1021 tttatcatga ttttgcctca atgatttttg tctattttaa acacaaaatt gtcattattg
     1081 acaatagagg cgctgatatt aaaaactgat aatagccaca tcaacagcaa acaaaaaata
     1141 ggcaaattta tgcgtcacat ggcaacttta tcggtattga ctatattagt ggggtgtagt
     1201 tcagcaacca ttaagccaac aagtaccatc acaacattag gggaaacatc tatgactgct
     1261 caacaaaatc aacaattag
//
LOCUS       DAOQMB010000690         1279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_92310_length_1279_cov_8.000000, whole genome shotgun sequence.
ACCESSION   DAOQMB010000690 DAOQMB010000000
VERSION     DAOQMB010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92310_length_1279_cov_8.000000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1222
                     /gene="dcm"
                     /locus_tag="PLY05_13240"
     CDS             68..1222
                     /gene="dcm"
                     /locus_tag="PLY05_13240"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HNC04304.1"
                     /translation="MNNNKIQSLRDCYAHDELYGMKQFVIRQWFDELQTKAYRPYTSI
                     ELFAGAGGLALGLEKAGFKAVLLNEFDKHACATLRANRPNWHVVEASICDVDFSTYCG
                     KVDFLSGGFPCQAFSYAGNKQGFDDTRGTLFFEFARAIKEVQPKVFLGENVRGLLNHD
                     NGRTLATIKNTIAELGYTLVPPQLLKAMYFQVPQKRERLFLVAVRNDLAAYMDYQWPA
                     QYHRVMTMRDAMFKGELYDCDVPESQGQLYPARKKAVLDLVPQGGYWRDLPDDIQREY
                     MKGSYFLGGGKTGMARRLSWDEPSLTLTCAPAQNQTERCHPEQTRPLTVREYACIQTF
                     PDDWQFMGAMSQQYKQIGNAVPVNLAYAMGRSLVRLLNQLETIEQSRACA"
BASE COUNT          368 a          225 c          294 g          392 t
ORIGIN      
        1 tatttagtgg cattaacctt aacaggcttt aaatggcgtg agttaaagca ttgatgatta
       61 gtgcattatg aataataata agattcaatc gttgagggat tgttatgcac atgatgagtt
      121 atatggcatg aaacaatttg ttatcaggca gtggtttgat gaactacaaa ccaaagccta
      181 tcgcccatat acctctattg aattatttgc aggcgcaggt ggtttagctt taggtttaga
      241 aaaagcagga tttaaagcgg ttttattaaa tgagtttgat aaacatgctt gtgcaacctt
      301 gcgagccaat cggccaaatt ggcatgttgt tgaagccagt atttgtgatg tggattttag
      361 tacttattgt ggcaaagtcg attttttgtc aggtggtttt ccctgtcagg ctttttctta
      421 tgcaggtaac aaacaaggtt ttgatgatac acgcggtaca ttgttttttg agtttgctcg
      481 cgccattaaa gaagtacaac ctaaagtgtt tttaggtgaa aacgtgcgtg gtttattaaa
      541 ccatgataat ggtcggactt tagctactat caaaaacacc attgcggagt taggttatac
      601 gttagtgccg ccacaattat taaaagcgat gtattttcaa gtaccacaaa agcgagagcg
      661 tttgtttttg gtggcggtgc gtaatgattt agccgcgtat atggattatc aatggccagc
      721 tcagtatcac cgagtaatga ccatgcgtga tgctatgttt aagggtgagc tgtatgattg
      781 tgatgtgcct gaatcacaag gccagcttta tccagcgcgt aaaaaagcag tattagacct
      841 cgtacctcaa ggtggatatt ggcgtgattt gcctgatgat attcaaagag aatacatgaa
      901 aggcagttat tttttaggcg gtggtaaaac aggtatggcg agacgcttat cgtgggatga
      961 acccagtttg accttgactt gcgcacctgc acaaaaccaa actgagcgat gtcatcctga
     1021 gcaaacacgg ccattaacgg tgcgagaata tgcctgtatc caaacctttc ctgatgattg
     1081 gcaatttatg ggcgcaatgt ctcaacagta taagcaaata ggcaatgctg tacctgttaa
     1141 tttggcctat gccatgggac gcagtttggt tcgtttactt aatcaattag agaccattga
     1201 acaatctcgg gcgtgtgctt aaaaatgacc caacttattc gacattggca aacattaaca
     1261 aatctttcat taccaccca
//
LOCUS       DAOQMB010000691         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_92579_length_1277_cov_20.474632, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000691 DAOQMB010000000
VERSION     DAOQMB010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92579_length_1277_cov_20.474632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..592)
                     /locus_tag="PLY05_13245"
     CDS             complement(155..592)
                     /locus_tag="PLY05_13245"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007114986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNC04305.1"
                     /translation="MFTLHPRLAEDTLPLGDFPLSSCRLMNDMHYPWLVLVPRVPSVR
                     EIYELSVRDQHQLLRESSWVSEQLAKYFRAEKMNVAAIGNLVPQLHIHHVVRYSNDAA
                     WPQPVWGKQPAMPYTPENLAVMRETLMLAFRGRDGVPFEWTLS"
     gene            complement(818..893)
                     /locus_tag="PLY05_13250"
     tRNA            complement(818..893)
                     /locus_tag="PLY05_13250"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(857..859),aa:Glu,seq:ttc)
     gene            complement(924..999)
                     /locus_tag="PLY05_13255"
     tRNA            complement(924..999)
                     /locus_tag="PLY05_13255"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(964..966),aa:Ala,seq:ggc)
BASE COUNT          384 a          280 c          273 g          340 t
ORIGIN      
        1 catcgtttta atgctttacg gggtaaggga aaattagcat ggaatgattt gccacatcat
       61 caacaatgga ttgcctctga gcaagcaatt cgtggtttaa tgggctaact ataaaatttt
      121 gctcaggttt ttagacctga gcaaaatgaa tcaattaact taatgtccac tcaaatggta
      181 caccatcacg accacgaaac gctaacatta aggtttcgcg catcacggct aaattttcag
      241 gtgtataagg catagcaggt tgtttgcccc atacaggttg tggccaagca gcatcgttac
      301 tataacgaac cacatgatga atgtgtaact gtgggactaa attaccaatt gcagcaacat
      361 tcattttttc ggcgcggaaa tacttagcta attgttcgct aacccaagat gattcgcgta
      421 ataattgatg ttggtcacgc actgacaact cgtagatttc acgaacacta ggcacacgag
      481 gcactaacac taaccaaggg tagtgcatat cattcattaa acggcaagag cttaaaggaa
      541 aatcacccaa aggtaaggtg tcttcggcta aacgaggatg aagggtaaac atacaaactg
      601 ttccttaaat tcagtaaaca ggatgaggca agtaacgtgt ttggcagaca agcgttaagc
      661 gcgtaaatcc tgataaaagc tgtttttaaa gtatttagta tgcaacgatt aagacttaca
      721 catcactgct tataatagaa cgaaaagttc gttcattgca tcgtcgtaca ttatttaaca
      781 gcataaaaaa acccgcaatc agcgggcttt cggaatctgg cgtccccacg gggattcgaa
      841 ccccggttac cgccgtgaaa gggcgatgtc ctaggcctct agacgatggg gacagagcaa
      901 atttttcgtc ataacgtcta aattggtgga gctaagcggg atcgaaccgc tgacctctac
      961 aatgccattg tagcgctctc ccagctgagc tatagcccca acatgcgtcg tgtcgaggcg
     1021 cgtatcttat tcagcagcaa caagattgtc aaatagttat cagcaaaaaa atactttttt
     1081 catggtgttt gctcgttttg tggtcaaacg tctaggttaa ctaattcggt atttgcgcca
     1141 attgctgtga tattgtcact caccacacgc tccgtgcaga aacgcagtag ataatgactt
     1201 cctcctgctt taaagcctgt ccctgataag ccttcaccac caaatggctg gcaagcaacc
     1261 tgtgcgccaa tttgatt
//
LOCUS       DAOQMB010000692         1277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_92584_length_1277_cov_7.603928, whole genome shotgun sequence.
ACCESSION   DAOQMB010000692 DAOQMB010000000
VERSION     DAOQMB010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92584_length_1277_cov_7.603928"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1117)
                     /gene="queG"
                     /locus_tag="PLY05_13260"
     CDS             complement(14..1117)
                     /gene="queG"
                     /locus_tag="PLY05_13260"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HNC04306.1"
                     /translation="MNLILYHLFVKMQKIPHLDAMQQLTQQIKNWGKDFGFQQVGISG
                     IDLGQHGEHLQAWLAKGYHGDLTYMASHGEKRWRPELLVENTQSIISVRMDYLQHKPT
                     PRTIPDYPQQGVIARYALGRDYHKTLRKRLQKFAEKISLEIDNLGYRAFVDSAPVLER
                     AVAEQAGLGWIGKNTMLINKQAGSFFFLGELFTDLPLIADAPVSHHCGSCQACLNICP
                     TQAIVAPYQLDARLCIAYLTIEYTGIIPEELRDKIGNRIFGCDDCQLICPWNRYAQLS
                     KEDDFKIRHHLDQLSLLELFAWSEQTYLHKTEGSPIRRIGYEGFLRNIAIGLGNAPYS
                     TAIVQALQEKKSIVSNLVQEHINWAINKQLLQQ"
BASE COUNT          372 a          246 c          264 g          395 t
ORIGIN      
        1 tattcgccaa gccttattgt tgtaataatt gcttgtttat tgcccaatta atgtgctctt
       61 gcaccaaatt ggatacgatt gatttctttt cttgcagtgc ttgcacaata gctgttgagt
      121 agggggcatt gcctaaacca atggcaatat tgcgtaaaaa gccctcataa ccaatgcgtc
      181 gaattggtga gccttcggtt ttatgtaaat aagtttgttc tgaccacgca aataattcga
      241 gtaatgacag ttggtctaaa tggtgacgta ttttaaagtc gtcttctttg gatagttggg
      301 cataacgatt ccacggacaa atcagttggc aatcatcaca accaaaaata cgattgccaa
      361 ttttgtcgcg taattcttca ggaataatgc ctgtgtattc aatggttagg taggcaatac
      421 ataaacgtgc atctaattga taaggagcaa caattgcttg tgttgggcaa atatttaagc
      481 atgcttggca tgagccacaa tgatgactaa caggggcatc ggcaatgagt ggcaagtcag
      541 taaataattc ccctaaaaag aaaaatgaac ctgcttgttt gttgatgagc atggtgtttt
      601 tgccaatcca gcccaaaccc gcttgttcgg caactgctcg ctctaaaaca ggcgcagaat
      661 cgacaaaagc tcgataacct aaattatcaa tttctaaact aattttttcg gcaaattttt
      721 gcagacgttt gcgtaaagtt ttatggtaat cacggcctaa agcataacgc gcaatcaccc
      781 cttgttgagg ataatcggga atggtgcgtg gtgtgggttt atgctgcaaa taatccatac
      841 gcacactaat aatgctttgg gtattttcga ctaataattc aggccgccag cgtttttcac
      901 cgtgactcgc catatacgtt agatcaccat gatagccttt ggctaaccat gcttgtagat
      961 gttcgccatg ttgtcccaaa tcaataccac taatccctac ttgttgaaag ccaaagtctt
     1021 tgccccaatt tttgatttgt tgggtgagtt gctgcatagc atcaagatga ggaatttttt
     1081 gcattttgac aaataagtga taaaggatta ggttcacagg agttacgcat tgcattgcaa
     1141 ttagcgcgtt ttgtgaacat aaaatcgttg agaacggttg ctatgtgaac aaataagcga
     1201 taaccgcgta actcctaata gcttttgatt atacacggaa ttttttatgt ctttgccagt
     1261 ttataacagc caacaaa
//
LOCUS       DAOQMB010000693         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_92978_length_1274_cov_13.931091, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000693 DAOQMB010000000
VERSION     DAOQMB010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92978_length_1274_cov_13.931091"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1274
                     /gene="gatB"
                     /locus_tag="PLY05_13265"
     CDS             <1..>1274
                     /gene="gatB"
                     /locus_tag="PLY05_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019457228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HNC04307.1"
                     /translation="SKIFSGSDTSFGAEANTQASLIDLGMPGVLPVLNQEAVNMAIRF
                     GLGVDAQIGRTSIFARKNYFYPDLPKGYQISQFEHPIVGKGHLDIVLEDGSTKRIGIT
                     RAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVSEPDMRSAKEAVAYVKAIHSIIR
                     YLGISDGNMAEGSMRCDCNVSLRYKGEEKFGTRREIKNVNSFKFIEKAINSEIEWQMD
                     ELESGRAIQQATVLYDPNTNKTRAMRSKEEANDYRYFPDPDLLPVVVSEEMINTVRAT
                     LPELPTAKKARFISDYALPDYDAGVLVAQRELADYFETVVKAANNQAKQASNWVMVEL
                     LGALNRAEKDITESPISAEQLGGLINRINDNTISGKIAKQVFGFMFDSQGKSADEIIA
                     EKGLKQETDTGAIEAVINEVVAANPQQLADYR"
BASE COUNT          381 a          260 c          285 g          348 t
ORIGIN      
        1 agtaaaattt tttcgggcag tgacacaagt tttggtgcag aagccaacac ccaagccagc
       61 ttaattgatt taggtatgcc tggtgtattg cctgtcctta accaagaagc cgtgaatatg
      121 gcgattcgtt ttggtttagg cgttgatgca caaattggtc gcaccagtat ttttgcccgt
      181 aaaaactact tttatcctga cctacccaaa ggttatcaaa tttcacaatt tgaacaccct
      241 attgttggca aaggtcattt ggatattgtt ttagaagatg gtagcaccaa gcgtattggc
      301 attactcgcg ctcacttaga agaagatgcg ggtaaatctt tgcatgaaga ttttgcagga
      361 atgacaggca ttgacttaaa tcgtgcaggt acaccgttat tagaaatcgt gtctgaacct
      421 gatatgcgct ctgccaaaga agccgttgcc tatgttaaag ccattcattc gattatccgt
      481 tatttgggta tttcggatgg caacatggct gaaggctcaa tgcgttgtga ctgtaacgtg
      541 tctttgcgtt ataaaggcga agaaaagttt ggcacacgcc gtgaaatcaa aaacgttaac
      601 tcctttaagt ttattgaaaa agcgattaac agcgaaattg aatggcaaat ggacgaatta
      661 gagtctggtc gtgccattca acaagccacc gtactttatg atccaaacac caacaaaacc
      721 cgcgccatgc gtagcaaaga agaagcgaat gattaccgct acttcccaga ccccgatttg
      781 ttgcctgtgg tggtgagtga agagatgatt aacacggttc gagcaacctt gcccgaattg
      841 cccaccgcta aaaaagcccg ttttatcagt gattacgcct tacctgacta tgatgcaggc
      901 gttttggttg ctcaacgcga attagccgac tattttgaaa cggtggttaa agccgccaac
      961 aatcaagcca aacaagcttc taactgggtg atggtagagt tattaggcgc attaaaccgt
     1021 gccgaaaaag acatcaccga atcgccaatt agtgctgaac aattaggtgg tttgattaat
     1081 cgtattaatg acaacacgat ttcgggcaaa attgccaaac aagtatttgg ttttatgttt
     1141 gactctcaag gcaaatctgc tgatgagatt attgccgaaa aaggcttaaa acaagaaacc
     1201 gatacaggtg ctatcgaggc ggtaattaac gaagttgttg cagctaaccc acaacaatta
     1261 gccgattatc gtag
//
LOCUS       DAOQMB010000694         1274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_93020_length_1274_cov_3.441345, whole genome shotgun sequence.
ACCESSION   DAOQMB010000694 DAOQMB010000000
VERSION     DAOQMB010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93020_length_1274_cov_3.441345"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..553)
                     /locus_tag="PLY05_13270"
     CDS             complement(146..553)
                     /locus_tag="PLY05_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04308.1"
                     /translation="MLKTSKMLKNNHVLVRVKAIITMGITALLTTCQPSYAIASHYKL
                     LKIHSFNCVDMVAVTSNNVLLSQKLSVSPLRHSGIFLPIVLQDGSAMPLCRVDTSKYK
                     TLLGKYEGRLLTAESETRHPNASQALAVFLNQS"
     gene            complement(556..786)
                     /locus_tag="PLY05_13275"
     CDS             complement(556..786)
                     /locus_tag="PLY05_13275"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04309.1"
                     /translation="MPSTDKVTLIRGNDLTLVDTPQAAEILKINERTLTNWRSTGKSP
                     RYIKCGRNVRYRLSDLMDWLDAQTRNHTGEGV"
     gene            complement(989..>1274)
                     /locus_tag="PLY05_13280"
     CDS             complement(989..>1274)
                     /locus_tag="PLY05_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04310.1"
                     /translation="QLVDENEALKAKVAELEQRLSEYKKLTTQTKNKVMPIIYGLCCL
                     YGNKGNPLIKSRASCESIASELMAKFGIEISDKGLENYYKEGRDILGEEK"
BASE COUNT          351 a          231 c          271 g          421 t
ORIGIN      
        1 gtttcatatt caccaatgac cacgctaaca ctgggaataa aatcagggtt aagctcggtt
       61 gtgggtacgc gaaaggagca aatgagggca aagggtttag acgcaacttg ggcgtgttgt
      121 tgaatatgat tcattttgga ttaccttaac tttgattgag aaacaccgct aacgcttgag
      181 atgcgttagg gtgtcgggtt tcactctcgg cagttaagag ccgcccctca tatttcccta
      241 aaagggtttt atattttgag gtgtcaaccc gacataaagg catagcacta ccgtcctgta
      301 gaacgatagg caaaaaaata ccactatgac gtagtgggga aacgcttaac ttttgagaga
      361 gaagtacatt attgctagtc accgccacca tatcaacaca attaaaacta tgaattttta
      421 agagtttata gtgtgatgct atggcataac ttggttgaca ggttgtcagt aaagcggtaa
      481 tgcccatcgt gataatggct tttaccctta ccaaaacatg attatttttt aacatttttg
      541 atgttttaag catgattaaa cgccctcccc tgtatggtta cgggtttggg catcaagcca
      601 atccataagg tcagataagc gataacggac attgcggcca catttgatat aacgtggact
      661 tttgcccgtg ctacgccagt tagttaaggt gcgctcgtta atttttagaa tttcggcggc
      721 ttggggagta tcaacaaggg ttaagtcatt accacgaata agggtaactt tatcggtgct
      781 aggcataatc aatcctctag gattttgttt agaggattcc aatgatatag cgtaatttgg
      841 ggtgcttttg gcaagggtta cactttgcat aggtgtcctc cagtgatgaa ggacttattc
      901 ttattttttg tgattgagtt ttgtggaatg ttccaaaata attaagagtt ttccattaaa
      961 aatgagtatt accaatgctt agatagggtt atttttcttc acctaaaatg tcacgtcctt
     1021 ctttatagta attttctaag cctttatcac tgatttctat tccaaatttt gccatcagtt
     1081 cgctggcaat agattcgcaa gaggctctac tttttattaa cggattacct ttattgccat
     1141 aaagacaaca aagaccataa ataattggca ttaccttgtt ttttgtttgg gtagttagtt
     1201 ttttgtattc gctaagacgt tgctctagtt cggcaacttt ggcctttaac gcttcgtttt
     1261 catcaaccag ttgt
//
LOCUS       DAOQMB010000695         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_93125_length_1273_cov_15.318555, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000695 DAOQMB010000000
VERSION     DAOQMB010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93125_length_1273_cov_15.318555"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..466
                     /locus_tag="PLY05_13285"
     CDS             137..466
                     /locus_tag="PLY05_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04311.1"
                     /translation="MKKTIVILLAQLAISGLAIAADNVKATKKSTTNSEVNISNGTDI
                     IGSKDAPLVLNIVSWKDKENYLPKNPLSASVLEESLAPIDRDVVSREVNYSRALQELS
                     PLSTVSP"
     gene            494..1123
                     /locus_tag="PLY05_13290"
     CDS             494..1123
                     /locus_tag="PLY05_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNC04312.1"
                     /translation="MSVIKFIQDGGFFMYPILLIMFIGLGIAIERIMFLSRVKKANKE
                     LWDAVYPSIQKGDTEKALKAVKDNETEIGRILEYGLTRNRTAKNVEDVETAMEEALME
                     VMPRLEARTPFIATFANVATLLGLLGTVQGLIVAFAAIANADPAQKGDLLSSSISVAM
                     NTTAFGLIVAIPLLLLYAFLQSRTGEVVESLEMASIKLLNALRQIRNNA"
BASE COUNT          395 a          237 c          259 g          382 t
ORIGIN      
        1 gcaaagaaaa gtccaaaaag gcaaaaaaag gtaaaaaaca agctgagaac gtgaccccaa
       61 gcactgccac ttcgggtcaa gaagccgtaa aatcagcccc catagagact aacgctgaga
      121 caggagtgca acaaccatga aaaaaacaat cgttatattg ttagcgcaac tagcgatttc
      181 tggtctagcc attgcggctg ataacgtcaa agccactaaa aaatcgacaa cgaatagtga
      241 agtgaatatt tccaatggta ccgatattat tggtagcaaa gatgcaccgt tggtgttgaa
      301 cattgtgtca tggaaagata aagaaaatta tcttcccaaa aatcctttgt cggcttcggt
      361 cttagaagaa agtttggctc ctattgaccg tgatgttgtg tctcgtgaag ttaactactc
      421 tcgtgcatta caagaactta gcccgctttc gaccgttagt ccttaatttt ttatttaaca
      481 ggagtacttt attatgtcag ttattaagtt tattcaagac ggtggtttct tcatgtaccc
      541 catcttattg attatgttca tcggtttggg tattgcgata gaacgtatca tgtttttaag
      601 ccgtgtgaaa aaagctaaca aagaattatg ggatgctgtt tatccttcta ttcaaaaagg
      661 cgatacagaa aaagctttaa aagccgttaa agacaatgaa accgaaattg gtcgtatttt
      721 agaatatggt ttaacacgta atcgtactgc taaaaatgta gaagatgtag aaaccgcaat
      781 ggaagaagcg ttaatggaag ttatgcctcg tttagaagca cgtacacctt ttattgctac
      841 atttgctaac gttgcaacat tgttgggctt gttaggtact gtacaaggtt tgattgttgc
      901 cttcgctgca atcgctaatg ctgacccagc gcaaaaaggt gacttattat ctagctctat
      961 ttcggtagcg atgaacacca ctgcgtttgg tttgattgtt gctattcctt tattattact
     1021 ctatgcattc ttacaaagcc gtactggtga agttgttgaa agcttagaaa tggcctcgat
     1081 caaactcctc aatgccttac gtcaaattcg taataacgct taattgtgta tcattgcgtt
     1141 aataaataga ggagacgatt atgcggtcac gcactaaaaa tcgtgacacg aatccggaac
     1201 tagacatcac ctcatttatg aatttgatgg tgattctaat cccattcttg ttgctagatg
     1261 cagtgtttac tca
//
LOCUS       DAOQMB010000696         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_93130_length_1273_cov_8.636289, whole genome shotgun sequence.
ACCESSION   DAOQMB010000696 DAOQMB010000000
VERSION     DAOQMB010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93130_length_1273_cov_8.636289"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1273)
                     /locus_tag="PLY05_13295"
     CDS             complement(<1..>1273)
                     /locus_tag="PLY05_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002116854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04313.1"
                     /translation="LVKHVTPELKAYRTFETAFFGLFHFGTENRSVILILMVAIAATT
                     TTLGLHHIALRPPSTKIDFKVYSIAMVAANVLLLASSISYWRLQISSGLPIEHPIINY
                     LWVGLFSILIAISIKSLIKPPADAKEGGSIGMALLSIPLYAFMALNAGLSFLFAGHYS
                     GLAIYLGQMMELSGVFLNLSLYIWVGMLLKQTRVVDLFLNIVRPWKFSPEVLTYIILL
                     AAAIPTAYTGASGIFVIAAGAVIYKEVYASGARRQYALAATAMSGSLGVVLSPCLLVV
                     VIAALNKEVTTSLLYGWGLKVFLLSSTMFLIASLLVSKEGFKFAKPAVALPESMRALV
                     PVSAYIVITIIVVMIYRFILDTKLDEFTAPMILPFIMLAIVWFDKIRRNPEDHAAPEV
                     KERRVGFEEGVRMATNDTIGHIGALIMLMALS"
BASE COUNT          463 a          268 c          231 g          311 t
ORIGIN      
        1 gacaaagcca tgagcatgat taacgcacca atgtgaccaa tagtgtcatt ggttgccata
       61 cgtacgcctt cttcaaaacc tacgcgacgc tctttaactt ctggagcagc atgatcttct
      121 ggattacggc gaattttatc aaaccacaca atagccaaca taataaatgg caaaatcatt
      181 ggtgcagtaa attcatctaa tttagtatct aatatgaagc gataaatcat taccacaata
      241 atagtgatta cgatatacgc agatactggc actaaagcac gcattgactc tggcaaagcc
      301 accgcaggtt tagcaaattt aaaaccttct ttagaaacca ataacgaggc aattaagaac
      361 atcgtagaac ttaacaagaa tacttttaag ccccaaccat ataataatga cgtggtcact
      421 tctttgttaa gtgcagcaat caccactact aacaaacaag ggcttaaaac aacccctaaa
      481 gaaccagaca ttgctgtagc tgctaaagcg tattggcgac gtgcaccaga ggcatacact
      541 tctttgtaaa tcaccgcacc cgcagcaata acaaaaatac ctgaagcgcc tgtataagcg
      601 gttggaatcg cagccgctaa aagaatgatg taagttaaaa cttctggcga gaacttccaa
      661 ggacgaacga tgttcaagaa taagtccact acacgggttt gtttgagcaa catacccacc
      721 caaatataca gtgacaagtt taagaatacg cctgataatt ccatcatttg gcctaaataa
      781 atcgccaaac ccgaataatg acctgcaaat aagaaactta aacctgcgtt taatgccata
      841 aacgcatata aaggaataga caataaggcc atgccaatac tgccaccctc tttagcatcg
      901 gcaggtggtt taattaacga tttaatacta atcgcaatta agatactaaa taagccaacc
      961 cataaataat taataatcgg atgctctata ggtaagccgg atgaaatttg taaacgccaa
     1021 taagaaatac tagaggccaa taacaagaca ttggcggcaa ccatcgcgat tgaatatact
     1081 ttaaaatcaa ttttagttga tggtggacgt aaagcaatat gatgcaagcc taaagtggta
     1141 gtggttgcag caatagcgac cattaaaatc aagatgactg aacgattttc tgtaccaaaa
     1201 tggaataaac caaaaaatgc ggtttcaaag gtgcgatagg cttttaattc aggcgtgacg
     1261 tgcttgacta aac
//
LOCUS       DAOQMB010000697         1273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_93136_length_1273_cov_5.758621, whole genome shotgun sequence.
ACCESSION   DAOQMB010000697 DAOQMB010000000
VERSION     DAOQMB010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93136_length_1273_cov_5.758621"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..961
                     /gene="cas6"
                     /locus_tag="PLY05_13300"
     CDS             74..961
                     /gene="cas6"
                     /locus_tag="PLY05_13300"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HNC04314.1"
                     /translation="MLPLLPLRFELKQHENLQLPQHAGSMLRGAFGHTFKRLACITKQ
                     KECKGCPLSQSCAYSQVFEGVINSDEQSSLPYVIHMPKEANRFIAAGQTWSFGMTLVG
                     KAINQLPIIAYAWQKTCEEGFSKDKSQAELKAIYHHDQIIYQPNKSLAAHTPSPVKAK
                     PLESVVHLDFVSPLRIQHNNYIVLNPEELTAPIVLIALAKRIQRLAELHATPINLDIA
                     QLLTSAQQISISTHLKRSTMTRYSNRQQRLMNLDGLIGRITLTGDLSLFSELLSLGEL
                     VHIGKNATMGLGQYKMSLN"
BASE COUNT          411 a          264 c          226 g          372 t
ORIGIN      
        1 ggcttggtaa acccattaaa gataatgaca agtggaaaaa acgcattaat gcacttaaac
       61 taaaggtatc ttaatgctcc ccctactccc cctacgcttt gaactaaaac aacatgaaaa
      121 cctgcaatta ccacaacatg caggttccat gttgcgtggt gcttttggtc acacctttaa
      181 aagactagct tgtattacca aacaaaaaga atgcaaaggt tgccctcttt ctcaatcctg
      241 cgcttatagc caagtgtttg aaggggtaat aaatagcgac gaacaatcct cattacccta
      301 tgtcattcac atgcccaaag aggctaatag atttatagct gctggccaaa catggtcttt
      361 tggtatgaca ctggttggaa aagccattaa tcaattaccg attattgctt atgcgtggca
      421 aaaaacgtgc gaggaaggat ttagcaaaga caaaagccaa gccgagttaa aagctattta
      481 tcatcatgac caaattattt atcagcccaa taaatcatta gccgcacata cgcctagccc
      541 tgttaaagcc aagcccttgg agtccgttgt tcatcttgat tttgtcagtc cgctacgcat
      601 acaacataat aattatattg ttttaaaccc agaagaactg actgctccta ttgtgttaat
      661 tgccttagca aaacgcatac agcgactggc agaactacac gccacgccta ttaatttaga
      721 cattgcgcaa ctattgacat ctgctcaaca aatttctatt agcacacatc ttaaacgttc
      781 aacaatgact cgttactcta atcgtcaaca gcgtttaatg aacttagatg gcttaattgg
      841 ccgaattact ttaacaggtg atttaagttt gtttagcgag ttgttgagct taggcgaatt
      901 agtccatatt ggcaaaaatg ccaccatggg attgggtcaa tacaagatgt cactcaacta
      961 aaatcgttat tttgcaaaag aaaaagccgc catactacca agcaaaagac agcccattta
     1021 aggcgtttaa tgagcaaact gcaagctcaa tttgaagaaa aagtcatggc ttaatgtgtg
     1081 gcaaaatggc taatttttct agccatttgt taaaaatatg gctactatag aggcttggga
     1141 acgcctccta aaactggtgt tttactctgt ttttgcagag caatgcgtca gttaatttgt
     1201 tctttaataa aatagccgaa aactattaaa accctatttt tattagtttt tattatgttc
     1261 cacgcgattg ttt
//
LOCUS       DAOQMB010000698         1269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_93727_length_1269_cov_7.162273, whole genome shotgun sequence.
ACCESSION   DAOQMB010000698 DAOQMB010000000
VERSION     DAOQMB010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93727_length_1269_cov_7.162273"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            8..295
                     /locus_tag="PLY05_13305"
     CDS             8..295
                     /locus_tag="PLY05_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC04315.1"
                     /translation="MDSLLTLNSAYDVQQSLAEAVRIRRKQKKWSRQELAERSGVPAA
                     TIKHFESTGQISLRQFLLLWQLLDSLDTILELTSLKQLLPESIDDVLKLRT"
     gene            297..>1269
                     /locus_tag="PLY05_13310"
     CDS             297..>1269
                     /locus_tag="PLY05_13310"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HNC04316.1"
                     /translation="MSLDIAQLDVFRVNSVGQEIKVGRLAQANRQVYFQYDEDYLKQG
                     HSLSSFRLKFSQELQINIDKSQTLQGVFADSLPDGWGRLLMDRWLRQQGYLPQQVSLM
                     SRLALVGDRGMGALRYRPVHPIPDDSLLNLHEIGQQAQAIFEGYHEDVLPQLVNVASS
                     GGARPKALIYLNPDNQHIVSQAQEGYEPWLIKFTSNSLADEGRCEAAYLTMAEKVGIT
                     VPEWRLHAHQQQAWLMVKRFDAPSALGRFHMHSAAGLLDANFREPSLDYEDLIKVTTK
                     LCQSVAAAKQQLLRALFNWYACNMDDHSKNWAFLQNDEGQWQISPMFD"
BASE COUNT          390 a          232 c          275 g          372 t
ORIGIN      
        1 tctttttatg gatagtcttt tgaccttaaa ttcagcctat gatgtacaac agagtttggc
       61 tgaagctgta cgtatacgcc gcaaacaaaa aaaatggtca cgccaagaat tggctgagcg
      121 tagtggtgtg cctgcggcaa ctatcaaaca ttttgaatca acaggtcaaa tctcattaag
      181 acaattttta ttgttgtggc aactgttaga ctccttagac actatccttg aattgacatc
      241 actcaaacag cttttgccag aaagtattga tgatgtatta aagttgagaa cctaagatga
      301 gcttagatat tgctcaactt gatgtatttc gtgtcaatag tgttggtcaa gaaatcaaag
      361 taggccgttt agctcaagct aacagacaag tgtattttca gtacgatgaa gactatctta
      421 aacaaggtca tagcctttca tcatttcgat taaaattttc ccaagaactg caaattaata
      481 tagataaatc acagacttta cagggcgtat ttgctgattc attacctgat ggttggggac
      541 gtttgttgat ggataggtgg ctacgtcaac aaggttattt acctcaacaa gtgagcctaa
      601 tgtcacgttt agcgttagtc ggcgatagag gcatgggcgc attacgttat cgtcctgttc
      661 atcctattcc tgacgattcg ttattaaatt tacacgaaat tggtcaacaa gctcaggcaa
      721 tttttgaggg atatcacgag gatgtgctac cacagttagt caatgttgct agctctggcg
      781 gagcgcgacc aaaagcacta atttatctga atcctgataa tcaacacata gtcagtcagg
      841 cacaagaagg ctatgagccg tggttgataa aatttacctc aaatagtttg gcagatgaag
      901 gacgctgcga agctgcgtat ttaaccatgg ccgaaaaagt aggtattacc gttcctgaat
      961 ggcgtttaca tgctcatcaa caacaagcat ggttaatggt caaacgtttt gatgcaccat
     1021 cagccttggg acgttttcat atgcacagtg ccgcaggatt attagatgca aactttagag
     1081 aaccgtcatt agactatgaa gatttgatta aagtgacgac aaaactctgc caatcagtgg
     1141 ctgcggctaa acaacaatta ttaagagcgc tatttaattg gtatgcgtgt aatatggatg
     1201 accatagtaa gaactgggca tttttacaaa atgatgaggg acaatggcaa attagtccga
     1261 tgtttgatg
//
LOCUS       DAOQMB010000699         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_94002_length_1267_cov_37.219472, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000699 DAOQMB010000000
VERSION     DAOQMB010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94002_length_1267_cov_37.219472"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1267)
                     /locus_tag="PLY05_13315"
     CDS             complement(<1..>1267)
                     /locus_tag="PLY05_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04317.1"
                     /translation="GKQTGEHIKAKALKSIVISDDAKARHIYTQVARLEDVLELKEGR
                     VLIVRAAMGEGKTQKVGRGFRNMAERNEQRFAALTHRSALVEELCDRLKLTSYNKVQE
                     RLNEGANAKDVYSFFGSCVHSIASPLIRSAFESCDVLFGDEAAQMLRSLESIYTQQTS
                     TARDSTAQDVYDLLVKTIRTAPKVVLCDAGANDELIEWLESILGNEKIHIVETSKKSG
                     DGINVTFNFANQQLQGEQAAITAIKARLKEGKKVWISVGTVKAGRLIAQALQGCGRGA
                     FIHSKTPTLTKKKFLESADRESLNYDYVIASPVISCGISVEHRDGHHFDDVFFIADGS
                     SVVPQDVLQMIRRVRYVKDVQIYTWLPFRQRLGDTTQEKRITDFYSLKKQITANVNWE
                     RQNFVAALCHTLESVGFNVSYQNVVPETG"
BASE COUNT          316 a          315 c          234 g          402 t
ORIGIN      
        1 ctgccagttt caggtactac gttttgataa cttacgttaa agcccacact ctctaaggta
       61 tgacaaagcg cggctacaaa gttttgacgc tcccaattaa cattggctgt aatctgtttt
      121 tttaagctgt aaaaatcggt tatgcgcttt tcttgtgtgg tgtcgcctag tctttgacgg
      181 aatggcaacc atgtataaat ctgtacgtct ttaacgtacc ttacgcggcg tatcatttgt
      241 agaacgtctt gaggtactac gctcgaacca tcagcgataa aaaaaacatc gtcaaagtga
      301 tgtccgtctc tatgctctac cgatatgccg caactgataa cagggcttgc tataacgtag
      361 tcataattta acgactctct atccgcactc tctaaaaact tctttttagt aagagtgggt
      421 gtttttgagt gaataaacgc accacgccca cacccttgca aggcttgcgc tatcaatcgt
      481 cctgctttaa ctgtacctac gcttatccaa acttttttac cctcttttaa gcgtgcctta
      541 atcgcggtta tggctgcttg ttcaccctgc aattgttggt tcgcaaaatt aaaagttacg
      601 tttatgccgt cacctgattt tttgctagtc tctacgatgt gtattttctc attgcctaag
      661 atagattcta gccattcaat caattcatca ttcgcgcctg catcacacaa gacaacttta
      721 ggggctgttc taatcgtttt aaccaataaa tcataaacat cttgggcggt tgaatctcta
      781 gctgtcgagg tttgttgcgt ataaatactt tctaaagaac gtaacatctg ggcggcttcg
      841 tccccaaaca acacgtcaca cgactcgaac gcgctacgga ttaagggcga ggcaatggaa
      901 tgaacgcacg aaccaaaaaa actataaaca tctttagcgt tcgccccctc gtttaatcgt
      961 tcttgtactt tgttgtaaga ggttaattta agtctgtcgc aaagctcctc tactaacgcg
     1021 ctacggtggg ttaatgctgc aaagcgttgt tcgttacgtt ctgccatatt cctaaaacca
     1081 cgccctactt tttgagtctt gccttccccc attgcagccc tgacaatcaa cacacgccct
     1141 tcttttagct ctagtacgtc ctctaatcgc gctacttgtg tgtatatatg ccgcgcctta
     1201 gcatcatcac taatcacaat gctctttaat gctttcgcct ttatatgctc gcctgtttgt
     1261 ttgcccc
//
LOCUS       DAOQMB010000700         1267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_94005_length_1267_cov_9.183168, whole genome shotgun sequence.
ACCESSION   DAOQMB010000700 DAOQMB010000000
VERSION     DAOQMB010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94005_length_1267_cov_9.183168"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..>1267
                     /locus_tag="PLY05_13320"
     CDS             115..>1267
                     /locus_tag="PLY05_13320"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019672010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNC04318.1"
                     /translation="MLDRTLILSVFVVASCGLAYELITAALASYLLGDSILQFSTIIG
                     CYLFAMGIGSHFAQYVPEDKVLDRFIEIELLVGLIGGLSATLLFVIFAWLSMPFRTVL
                     YALVLIIGMLVGMEIPLVMRTLNQHKAEFSALISRVLTFDYLGALAVSLLFPLILAPK
                     LGLARSALLFGISNAAVAAWTIYRFKDQLQSVSRQRNRAILSLGFLILAFVGSDKLVS
                     WSEQQIFADPIIHAQSTPYQRLIVTRWQDDLRLYINGNLQFSSRDEYRYHEALVHPAL
                     ATRPQAKTVLVLGGGDGLAVREILRYPHIEHVTLVDLDPAMTSLFSTNAELTKLNHNS
                     LLSTKVTVVNADAGHWLEQQQQPFDVIIADLPDPSNFGLGKLYSVPMYRL"
BASE COUNT          320 a          268 c          258 g          421 t
ORIGIN      
        1 aataaataga taatatagtt ataaatatac gttgataaca tcgtgtcgtt tccttggcaa
       61 agtatttcag agtattatgc cgcacttctt aacctctgcc tataacaact atttatgctc
      121 gaccgtaccc taattttatc tgtttttgtc gtggcatctt gtggtttagc ctacgagcta
      181 atcaccgctg ccttagccag ctatctatta ggcgattcga ttttacaatt ctccaccatt
      241 attggctgtt atttatttgc catgggcatt ggctcgcatt ttgcacaata tgttcccgaa
      301 gataaagtgt tagatcgttt tatcgaaatt gaactattag tgggtttaat tggtggttta
      361 tcggcaactt tgttatttgt gatttttgca tggttgtcga tgccgtttag aacggtgttg
      421 tatgcccttg tgttgattat tggcatgtta gtgggcatgg aaattccact ggtgatgcgc
      481 actcttaatc aacacaaagc cgaatttagt gcgttaatca gtcgggtatt aacctttgat
      541 tatttaggtg cgttagcggt ttcgctactc tttccactca ttttagcccc caaacttggc
      601 ttggcgcgta gtgccttatt atttgggatt agtaatgccg ccgtagccgc atggacaatt
      661 tacaggttta aagatcaatt acagtcggtt tctcggcagc gtaatcgggc aattttaagt
      721 ttaggctttt taatattagc ctttgttggt agtgataaat tagtctcttg gtcagagcaa
      781 caaatttttg ccgacccgat tattcatgcc caaagcacac cctaccaacg tttaattgtt
      841 acccgttggc aagatgactt gcgcttatat attaatggca atttgcaatt ttcgagtcga
      901 gatgaatacc gctaccacga agccctcgtt catcctgcct tagccacacg accacaagca
      961 aaaaccgtat tagtgctagg tggcggtgat ggtttggctg tacgtgagat tttgcgttat
     1021 ccacacattg aacacgtcac cttagtcgat ttagaccctg cgatgaccag tttatttagc
     1081 accaatgccg aattaaccaa actaaaccat aactctttac ttagtacaaa agtcaccgtg
     1141 gtcaatgccg atgcaggcca ttggctagaa caacagcagc aaccatttga tgtgattatt
     1201 gccgacctgc cagacccttc taactttggc ttgggcaaat tatattctgt acccatgtac
     1261 cgattag
//
LOCUS       DAOQMB010000701         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_94690_length_1262_cov_16.756421, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000701 DAOQMB010000000
VERSION     DAOQMB010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94690_length_1262_cov_16.756421"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1262)
                     /locus_tag="PLY05_13325"
     CDS             complement(<1..>1262)
                     /locus_tag="PLY05_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020442524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HNC04319.1"
                     /translation="PQADGTYKLTGTKIFISAGEHDLTENIVHIVLARLPDAPKGTKG
                     ISLFIVPKFIPDAQGNVGERNAVSCGSIEHKMGIRASATCVMNFDNATGYLIGLPNKG
                     LEAMFTFMNTARIGTAVQGVAHAELSYQGALAYSKERRSMRALSGKKEPDQIADALIY
                     HADVRRMLLTQKAIAEGGRAMIYFASQYADHMTNGIIENDKAKYEHWDDKLGFFTPIL
                     KGFLTELGLESANIGMQVFGGHGYIKEHGMEQIARDARIATLYEGTTGIQALDLLGRK
                     VLLQSRGKAVRDFTADVLKFCGRHARDKNLRRYVWDLTKLCAQWNYLTTRIMLMARKD
                     RDIVSAACNDFLMYSGYVTMAYFWALQASVAYEKLANGGAEQPEFYQAKIQTAEFYFE
                     RLLPRASGHAGSMLSPSKSLMQLDKDAFV"
BASE COUNT          388 a          278 c          236 g          360 t
ORIGIN      
        1 acaaacgcgt ctttatctaa ttgcattaag gacttagagg gtgacaacat gctacctgca
       61 tgacccgaag cacgtggtaa taaacgctca aaataaaatt cagcggtttg aattttagct
      121 tgataaaact caggctgttc tgcaccacca ttggctaatt tttcataagc aacagaagct
      181 tgtaatgccc agaaataggc catagttaca taaccagaat acatcaagaa atcgttacaa
      241 gcggcactga cgatgtcacg atctttgcga gccattaaca tgatgcgtgt ggttaagtag
      301 ttccactgag cgcataactt ggttaagtcc caaacgtaac gacgtaagtt tttgtcacgt
      361 gcatgacgac cacagaactt caatacatcg gcggtaaagt cacgtactgc tttgccacgg
      421 ctttgcaaca acactttacg acctaataag tctaatgctt gaatacctgt tgtaccttcg
      481 tacaatgtcg caatacgcgc atcacgtgca atttgttcca tgccatgttc tttaatataa
      541 ccatgaccac caaacacttg cataccaatg ttggcacttt ctaaacctaa ttcggttaag
      601 aaacctttga gaattggggt gaagaaacct aatttgtcat cccaatgttc atattttgct
      661 ttatcgtttt cgataatgcc attggtcata tggtcagcat attggctagc aaaataaatc
      721 atcgcacggc caccctcagc aatcgctttt tgggttaaca acatacggcg tacatcagca
      781 tgataaatta aggcatcagc aatttgatct ggttcttttt taccagacaa agcacgcatt
      841 gaacgacgct ctttagagta agctaatgcg ccttggtaag ataattctgc atgggcaaca
      901 ccttgaaccg ctgtaccaat acgtgcagtg ttcatgaagg taaacatcgc ttccaaacct
      961 ttgtttggta aaccgattaa ataacctgtt gcattatcaa agttcatcac acaggttgca
     1021 gaagcacgaa tccccatttt gtgttcaata gaaccgcaac taacggcatt acgttcacca
     1081 acattgcctt gagcatctgg aataaacttt ggcacgataa acaaagaaat acctttagta
     1141 ccttttggtg catctggtaa acgtgctaaa acgatatgaa cgatattttc agttaaatca
     1201 tgttcacccg cagaaataaa gattttagta cctgttaatt tataagtacc gtcggcttgt
     1261 gg
//
LOCUS       DAOQMB010000702         1262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_94692_length_1262_cov_8.454018, whole genome shotgun sequence.
ACCESSION   DAOQMB010000702 DAOQMB010000000
VERSION     DAOQMB010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_94692_length_1262_cov_8.454018"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1262
                     /locus_tag="PLY05_13330"
     CDS             <1..>1262
                     /locus_tag="PLY05_13330"
                     /inference="COORDINATES: protein motif:HMM:NF021424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNC04320.1"
                     /translation="FSQGLNQVIGHVLPVSWNEKAETWQTGEWFLRGKHCYLIPGDSP
                     LGYRLPLESQPWVSKEDYPYINPADPSATQGPLKSYTAIKQQFMGDKTHTLSYNTPPK
                     AKKSAHELTRTALCAEPRDGRLYVFMPPLAELEHYLALVSAIEATAQALNHPIILEGY
                     EPPLDTRLTHFRVTPDPGVIEVNIHPSSSWQELVDKTTHLYENAHLCRLTTEKFMTDG
                     RHTGTGGGNHFVLGGATVNDSPFLRRPDVLRSLISYWHNHPSLSYLFSGLFIGPTSQA
                     PRIDEARNDSVYELELAFQQFPAVGKSCPPWLIDRLVRNILIDVTGNTHRAEFCVDKL
                     YSPDSSSGRLGLLELRAFEMPPHSQMSLAQQLLIRALIARFWQEPYQPARLTRWGTEL
                     HDRFMLPYFVKQDFSDVLTELRDSGYDF"
BASE COUNT          341 a          265 c          271 g          385 t
ORIGIN      
        1 tttagtcaag gtttaaatca agtcattggt catgtgttac ctgttagttg gaatgaaaaa
       61 gccgagacat ggcaaacagg tgaatggttt ttgcgtggca aacactgtta tctgattccg
      121 ggtgattcac cattaggcta tcgtttgcct ttagagtcac aaccgtgggt gagcaaagaa
      181 gattatccct atattaatcc tgccgacccc agtgctactc aagggccatt aaaaagttat
      241 accgccatta aacaacagtt tatgggtgat aaaactcata ctttaagcta caacacacca
      301 ccaaaagcga aaaaatcagc ccatgaatta acacgcaccg ctttatgtgc cgagccgcgt
      361 gatggccgat tgtatgtgtt tatgccgccg ttagccgaat tagagcatta tttagcttta
      421 gtatctgcaa ttgaagcgac tgcacaagcc ttaaaccacc cgattatttt agaaggttat
      481 gaaccgcctt tagatacacg cttaacccat tttagagtca cgcctgatcc gggggtgatt
      541 gaagtcaata ttcatccatc tagttcttgg caagaattag tcgataaaac cacacatttg
      601 tatgaaaacg cccatttatg ccgtttaacc accgaaaaat ttatgactga tggccgtcat
      661 acaggcacag gtggcggtaa tcattttgtc ttgggtggag caacggtgaa tgattcaccg
      721 tttttacgcc gtcccgatgt gttacgcagt ttaatttcgt attggcacaa tcatccttcg
      781 ttatcgtatt tattttcggg attatttatt ggcccaacct cacaagcacc acgcattgac
      841 gaggctcgca atgattctgt ttatgaatta gaactggctt ttcaacagtt tcctgcggtt
      901 ggcaaaagct gtccgccgtg gttaattgat cgtttagtgc gtaatatttt aattgatgtc
      961 acaggtaata cccatcgtgc cgaattttgt gtcgataaat tgtactcgcc cgacagcagc
     1021 agtggccgtt taggcttgct tgagttacgt gcttttgaaa tgccaccaca ctcacaaatg
     1081 agtttggcac aacaattgtt gattcgcgca ttaattgctc gtttttggca agagccgtat
     1141 cagccagctc gtttaacgcg ttggggtaca gagctgcatg atcgttttat gttgccttat
     1201 tttgttaaac aagattttag tgatgtgtta accgaattaa gagactcagg ttatgacttt
     1261 aa
//
LOCUS       DAOQMB010000703         1259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_95150_length_1259_cov_14.048173, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000703 DAOQMB010000000
VERSION     DAOQMB010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95150_length_1259_cov_14.048173"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /gene="pabB"
                     /locus_tag="PLY05_13335"
     CDS             <1..1259
                     /gene="pabB"
                     /locus_tag="PLY05_13335"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNC04321.1"
                     /translation="FWPFSFKDADYSCLALLPSWLLIKHQNQLILLTRETNFSYKKEI
                     VSSNFLDYLKQHFANSACPQYPITKGFNGGLVGWLSYPSTSQQHEIDFPSFCFGFYDC
                     FIQTDKHGISLLYTSSEDLTNTILYRIKNSHLSGFSLLSSFKQLMSEEEYQQAFTQVH
                     NYLLSGDCYQVNLAQAFKADCQGVASGAFERLLALSNPPYAAYSHTPFGEILSLSPEL
                     FLQFCEDGSVLTRPIKGTRPRKQNSVEDQAQFDDLQDHPKDRAENLMIVDLLRNDLAK
                     HAEIGSVKVPKLFKVESFPQVHHLISDISCQLKTGSHPLDLLFDAFPGGSITGAPKKR
                     AMEIIDELENCQRAIYCGSMGYLSVTGRGQWNITIRTLLKVKDSLYAWAGGGIVADSV
                     CESEYQECFNKIGAILQLLEDEFLAD"
BASE COUNT          373 a          200 c          254 g          432 t
ORIGIN      
        1 atttttggcc tttttctttt aaagatgctg attattcttg tttagctttg ctgcctagtt
       61 ggttattaat caaacaccaa aatcaactta ttttattaac tagagaaact aatttttcat
      121 ataaaaaaga aatagttagc tccaattttt tagactattt aaaacaacat ttcgcaaaca
      181 gtgcttgccc ccaataccct attacaaaag gctttaatgg cggtttggtg ggttggttaa
      241 gttacccttc gacatcgcaa caacatgaaa ttgattttcc gagtttttgt tttggctttt
      301 atgattgttt tattcaaacg gataaacatg gcatcagcct tttatatacg agtagcgaag
      361 atttaactaa cacaattctt tatagaataa aaaactctca tctctcaggc tttagtttat
      421 taagttcttt taaacagcta atgtctgaag aggaatatca acaggcattt acacaagttc
      481 acaattattt gttaagtggt gactgttatc aggtgaattt agcacaagct tttaaggccg
      541 attgtcaagg cgttgcaagt ggtgcttttg agcgtttgtt agccttgagc aatcctcctt
      601 atgcggcgta tagccataca ccatttggtg aaattttgag cttatcaccc gaactttttt
      661 tacaattttg tgaagatggc tcagtgttaa ctcgcccaat taaagggact cgtccacgta
      721 aacaaaattc agttgaagat caggctcaat ttgacgattt acaagatcat cccaaagatc
      781 gagcagaaaa tttaatgatt gttgatttgt tgcgtaatga tttagccaaa catgctgaaa
      841 ttggttcggt taaagtgcca aaattattta aagttgaatc ttttccacag gttcatcatt
      901 taattagtga tattagctgc caattaaaaa caggttcgca tcctttagat ttgttgtttg
      961 atgcttttcc tggtgggtcg attactggcg cacccaaaaa acgggcaatg gaaattattg
     1021 atgagctaga aaactgccaa cgagccattt attgtggcag catgggttat ttgagtgtaa
     1081 cgggtcgtgg ccaatggaat attacgatta ggacgttatt aaaggttaaa gatagtttgt
     1141 atgcgtgggc tggtggaggc attgttgctg attcggtatg tgagtcagaa tatcaagagt
     1201 gctttaataa aattggggca attttgcagt tgttagaaga tgagttttta gcggattaa
//
LOCUS       DAOQMB010000704         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_95597_length_1256_cov_14.089925, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000704 DAOQMB010000000
VERSION     DAOQMB010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95597_length_1256_cov_14.089925"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..140)
                     /locus_tag="PLY05_13340"
                     /pseudo
     CDS             complement(1..140)
                     /locus_tag="PLY05_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008941489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            535..>1256
                     /locus_tag="PLY05_13345"
     CDS             535..>1256
                     /locus_tag="PLY05_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNC04322.1"
                     /translation="MINATARLVPLPFEFHQLNKSGLHAKPHHDLLGFTVARQHMALK
                     TIPLFTWSEPRIRTLHFTWLAFFITFLIWFAHAPLMPVLKQYFGLTPEQVKAILMLNV
                     AITIPARILIGVLVDKFGPRKSYSFLLAFCGALCLLFAAAQSFEWLAVMRFLLGFVGA
                     GFVIGIRLVSEWFPAREVGIAEGIYGGWGNFGAAVASMTMPLLALAFGGDDGWRYAIG
                     TTGVIALIYSVIFYRNVSDTPKG"
BASE COUNT          347 a          258 c          251 g          400 t
ORIGIN      
        1 gacaacataa tacggttgta attgccgtgc ggctctttgc caatgacagt aatgtcatat
       61 aaatcaggcg cgagcgttaa taattcctca acggtacgca tacctgccat accattaccg
      121 accacgacta atttttgttt gttcattgcc aatgcctatt atctaggact tacatgaata
      181 aagacgcaaa agaccaagag cttccaacac tagatttaaa aaatctaatt gtctctctta
      241 gccacgttgc taaaggatga gataaatttt tcagaagttt tagtcattac gctatcagca
      301 cgtaagtcta aaaaagaatt ttctgaaatt agttaagcaa aatatatgcc atattaaaaa
      361 caaggggttt attatgctat ttaatcgaaa aatgcaccat taccatgcct tgcctcataa
      421 aaatgcacca aatgcaccac aagcaaacaa ttcgcttaaa gtaatctttt taaggcaaag
      481 tcaataatca caaggtttta aagcaaatgg cataacaatt gcttagggct taacatgatt
      541 aacgcaacgg cgcgtctagt ccctcttccc tttgagtttc atcagctcaa taaatcaggg
      601 ttacacgcaa agccgcatca tgatttattg ggttttaccg ttgcgaggca gcatatggct
      661 ttaaaaacta tcccactctt tacatggagt gagccacgaa tccgtaccct acacttcact
      721 tggctagcat tctttattac cttcttaatt tggtttgctc acgcaccgtt aatgcctgta
      781 ttaaaacaat actttggtct gacacctgaa caagtcaaag cgattttgat gcttaatgtg
      841 gcaattacca ttcctgcgcg gattttaatt ggcgtgttgg ttgacaaatt tggcccacgc
      901 aaaagttata gtttcttatt agccttttgt ggagcgttgt gcttgttatt tgccgccgct
      961 caaagttttg aatggttagc cgtcatgcgc tttttattag gctttgttgg tgcaggtttt
     1021 gtgattggca ttcgtttagt cagtgaatgg ttccctgctc gtgaagtcgg tattgccgaa
     1081 ggtatttatg gtggctgggg taactttggt gctgccgttg ccagtatgac catgccctta
     1141 ttagccttag cctttggtgg tgatgacggc tggcgttatg ccatcggcac aacaggtgtc
     1201 attgccttaa tttacagtgt gattttctat cgtaatgtgt ctgatacacc taaagg
//
LOCUS       DAOQMB010000705         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_95598_length_1256_cov_13.040799, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000705 DAOQMB010000000
VERSION     DAOQMB010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95598_length_1256_cov_13.040799"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1256)
                     /locus_tag="PLY05_13350"
     CDS             complement(<1..>1256)
                     /locus_tag="PLY05_13350"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HNC04323.1"
                     /translation="ISLAPSFDDAGLISLKLTASDGLLSTTQQFDLNVRNVNRAPTQI
                     NNSAFEIDEDSGDVVIDVLSRVQDLDKDNLTVRNVSLASGQGSITLNQNQQIIFTPTQ
                     DFNGQASITYEVIDNQGGVLAITETITVKSINDAPRLVSTPAILADGLEDTDYQLTTL
                     SLLQGFNDVDNDNLVVENLTSSQGTFINNGNGIFVFKPQANINGDIQFSYQISDSKGG
                     VIEASSHFNTKAVNDAPFVTIKNPSSTARIREDATSVLVALAFGVILEDVDADNFTRL
                     EVSMQQFTPKDLLSVIVDGTNLQASYNAILGKLVVTGSDSLANYQKVLDSLALSTTAE
                     QGATERSIQITISDGKVAHSNQFVVPVDYVQDETHQGGTGNDTLNGSDGNDTLRGGEG
                     DDILNGGNGNDLLFGEGGNDILNGGDG"
BASE COUNT          391 a          277 c          194 g          394 t
ORIGIN      
        1 ccatcaccac cattaagaat atcgttacca ccctcaccaa atagcaaatc gttaccatta
       61 ccgccattta aaatgtcatc tccttcacca ccacgtaaag tatcattacc atctgaacca
      121 tttaaggtgt cattacctgt tccaccttga tgagtttcat cttggacata atcaacaggc
      181 acaacaaact gattagaatg cgctactttt ccatcactaa tggtaatttg aatactacgc
      241 tcagttgccc cctgctcggc tgtggtactt agtgctaaag aatctaaaac tttttggtag
      301 tttgctaaac tatcactgcc tgttaccact aatttaccca aaatagcgtt atagctagct
      361 tgcaaatttg tgccatcaac aatcactgat aataagtctt ttggcgtaaa ttgctgcata
      421 ctcacttcta aacgagtaaa gttatcagca tcaacatcct ctaaaattac accaaaagct
      481 aaagcaacta aaacactagt agcatcttca cgaattcgtg cagtacttga cgggttttta
      541 atagtaacga atggagcatc gttaaccgct ttcgtattga aatggctaga tgcctcaatc
      601 accccaccct tactatcact aatttgataa ctaaactgaa tatcgccgtt gatatttgct
      661 tgcggtttaa agacaaatat gccgttacca ttattaataa acgtaccttg agagcttgtg
      721 aggttttcta cgactaaatt gtcattatct acatcgttaa aaccttgtaa taacgataat
      781 gtagttaact gataatccgt gtcctctaag ccgtcagcca atatagctgg cgtacttact
      841 aaacgtggcg catcattaat tgacttaaca gttatcgttt ctgtaattgc caaaacaccg
      901 ccttgattat caattacctc ataagtaatt gatgcctgac cattaaagtc ttgtgtaggt
      961 gtaaaaataa tctgttggtt ttgatttagt gtaatactgc cttgtcccga agctaagctg
     1021 acattacgaa cagttaaatt atctttatct aaatcctgta ctcgcgataa aacatcaatg
     1081 acaacatcac cactatcttc atctatttca aatgcagaat tgttgatttg tgtaggcgca
     1141 cgattgacat tacgcacatt taaatcaaat tgttgtgtcg tacttaataa accatcgctc
     1201 gccgttagtt ttaatgaaat caagcctgca tcgtcaaacg agggtgctaa tgatat
//
LOCUS       DAOQMB010000706         1256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_95606_length_1256_cov_5.353872, whole genome shotgun sequence.
ACCESSION   DAOQMB010000706 DAOQMB010000000
VERSION     DAOQMB010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95606_length_1256_cov_5.353872"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..>1256)
                     /locus_tag="PLY05_13355"
     CDS             complement(156..>1256)
                     /locus_tag="PLY05_13355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative CRISPR-associated protein"
                     /protein_id="HNC04324.1"
                     /translation="MPSLILSTCGTSLLTNGGIPDDLRRLLNKHANSRDWSVMPANEA
                     HTLQQHANSRQQSLLAADEQQVRRLSAELNGLLSWQQRSETPASPQDMYLLLATDTAL
                     GQATAQSVCAWLQKQGHSATIISAAGLNTASLNSFRQALSGLVKDLHEQLSSYKASGY
                     SINFNLTGGFKGLNGFLQALSTIYADNAFYLFEGSSEVMLIPSLPLTLDAEQVIKQNL
                     RAIRRLSQNLPVIAKDCSNIPELLLFSIDQERVLSEWGELLWRNSQSKLYKQGLYPSI
                     SERVIFGEAFEASTRNKSPELLAIINQRIDDLAVYAEGDCKQALKSLDPKQLQGSQHR
                     SLWECDLDDHHRIFMRKDGHTFFLEKVDRALH"
BASE COUNT          334 a          252 c          281 g          389 t
ORIGIN      
        1 attctttttg tttggtaata caagctagtc ttttaaaggt gtgaccaaaa gagccacgca
       61 acatggagcc tgcatactca gggaattgta aattttcgtg ttgtttaact tctattttta
      121 aatcaagtat aggaaacata tcgttctcaa tagttttagt gtaaggctct atctactttt
      181 tctaaaaaga aggtatggcc atctttgcgc ataaatatac gatgatggtc atctaaatca
      241 cattcccaca agcttctgtg ttggctacct tgtaattgtt tggggtcaag agatttgagt
      301 gcctgtttac aatcgccttc ggcataaacg gctaagtcat caattcgttg gttgataatg
      361 gcaagtagct ctggactttt gtttctagta ctggcctcaa acgcttcgcc aaagatcacc
      421 cgctcagaaa tactggggta caaaccttgt ttatagagct tactttggct atttcgccat
      481 aaaagctcac cccattccga taatacgcgc tcttggtcaa tactaaataa tagtaactca
      541 ggaatgttgg agcaatcttt tgctattaca ggcagatttt gacttaagcg acgaatagca
      601 cgtagatttt gtttaatcac ctgttcggca tctaaagtta gtggcaaact gggaatcagc
      661 atcacttcac tactgccttc aaacaaataa aaagcgttat cggcataaat cgtagataac
      721 gcttgtaaaa agccatttag ccctttaaaa cctcctgtta aattaaagtt aatactatag
      781 ccactggctt tataactact gagttgttcg tgtaagtctt tgactaagcc tgatagggct
      841 tgtctaaaac tattcaatga agcagtgttt aaacctgctg ctgaaataat ggttgcacta
      901 tgaccttgtt tttggagcca cgcacacacg ctttgtgcag tggcttgccc taatgcagta
      961 tctgtggcca ataataaata catatcttgg gggctggcgg gcgtttcgga gcgttgttgc
     1021 caacttagca gtccattcag ttcggcactc aaacgacgaa cttgctgttc gtctgccgcc
     1081 aagaggcttt gttggcggct attggcgtgt tgttgcaggg tatgggcttc gttggcaggc
     1141 attactgacc aatcacgact gttggcgtgt ttgttgagta aacggcgtaa gtcatcgggg
     1201 attccgccgt tggtcagtag gcttgtgccg caagtagaaa ggatgaggga gggcat
//
LOCUS       DAOQMB010000707         1255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_95752_length_1255_cov_9.795000, whole genome shotgun sequence.
ACCESSION   DAOQMB010000707 DAOQMB010000000
VERSION     DAOQMB010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95752_length_1255_cov_9.795000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..>1255
                     /locus_tag="PLY05_13360"
     CDS             202..>1255
                     /locus_tag="PLY05_13360"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HNC04325.1"
                     /translation="MNHQLTVKAAQLSHQGPKDSNDDAIGLHLPQGHGIASQTLAVAI
                     ADGLSSAEGGRIAAETSVTNFLSDFFATPASWSIKTSGFKVLEALNRWLHGQGQHYHN
                     HHQAHLTTFTALVIRGQTAHVFHIGDSRLWRLRGDEWECLTNDHALSTRNGNQLTRAL
                     GMDWRIDIDYQEFNVEVGDIYLLSTDGIHQFIPPKELKAIVASRSETLEARCEELIAC
                     AESHHSDDNRSCQIIDIIDLPNNNQHLALDLQKLPPLPPELKIGDCIDDYIIEAELQA
                     NARSHVYLVKDLHDHKLYILKTPSHNLADDIEALQAFQREDWIGQRVHHPDIVKTYAP
                     TRPRHYLYLIQEHLQGQ"
BASE COUNT          385 a          230 c          261 g          379 t
ORIGIN      
        1 tagccagtgc attcttctta gatgaaccta aaggtcacat ggttgaaaaa gacgaacatg
       61 gcaatgtgca tttgattgcg gttgaataaa ctgcttaaag tgagcgagac aaagcagaca
      121 aggaggtctg cttttttatt agataggcat agtttgtgta tatggataga tcttaatctt
      181 tattgtcgag tgtttaagga tatgaatcac caattaacgg ttaaagcggc tcaactttct
      241 catcaagggc ctaaagacag caatgatgat gcgattggtc tgcatttacc acaaggacat
      301 ggcattgcta gtcaaaccct cgctgtggct atcgccgatg gtttaagcag tgccgaaggt
      361 ggccgaattg ctgccgaaac cagtgtcact aattttttaa gcgatttttt tgctacccct
      421 gctagttggt cgatcaaaac atcaggtttt aaagttttag aagccttaaa tcgttggctg
      481 catggtcaag gtcaacatta tcataatcat caccaagcac acctgacgac attcaccgcc
      541 ttagtgatta gaggtcaaac ggctcatgtg tttcatattg gtgatagtcg tttgtggcgt
      601 ttacggggag acgaatggga atgtttaacc aacgatcacg cattaagtac acgcaatggc
      661 aatcaactaa cgcgtgcttt aggtatggat tggcgtattg atattgatta tcaagagttt
      721 aatgtggaag taggtgatat ttacttatta tctacagatg ggattcatca atttattccg
      781 cccaaagaat taaaagcgat tgtcgctagt cggagtgaaa ccttagaagc gcgttgtgaa
      841 gaattaattg cctgtgccga aagtcatcat agtgatgata atcgctcttg tcagattatt
      901 gatattattg atttgcctaa caataaccaa catttagcat tagacctaca aaaattacca
      961 ccgttgccac ctgaacttaa aataggtgac tgtattgatg attatattat tgaagcggaa
     1021 ttacaagcga atgcccgcag ccatgtttat ttagttaaag atttacatga ccacaaacta
     1081 tatattttaa aaacaccttc acataacttg gcagatgata ttgaagcctt acaagctttt
     1141 cagcgtgaag attggatagg tcaacgagta catcatcctg atattgtcaa aacttatgct
     1201 cctactcggc cacgccatta tttatattta attcaagaac atttacaagg tcaaa
//
LOCUS       DAOQMB010000708         1251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96319_length_1251_cov_9.528428, whole genome shotgun sequence.
ACCESSION   DAOQMB010000708 DAOQMB010000000
VERSION     DAOQMB010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96319_length_1251_cov_9.528428"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="PLY05_13365"
     CDS             <1..839
                     /locus_tag="PLY05_13365"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC04326.1"
                     /translation="ERTNYYAFGQDEWQIANTLQLTTGLRYDHFSDFGSTVNPRLALI
                     WATTPDFTTKLLYGRAFRAPAIAELFVTSNPVQLGNPDLNPETIDTYELAFSHQASSQ
                     LLYSANIYYYKIRDYIVFVPISQFANQTQNSGKRHGYGLSLEADYSMTDNFRLVGNYS
                     YQQAKDDNTHEAAGESPDHQLYARGEWQFLPSWLFSPQVNVIGQQKRAANDARDAVDA
                     YTTVDFTLRKKNNIQPLSFALSVHNVFNADVREPSLASGAIVDDYPMAKRSVMAEFNY
                     QF"
     gene            complement(860..>1251)
                     /locus_tag="PLY05_13370"
     CDS             complement(860..>1251)
                     /locus_tag="PLY05_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04327.1"
                     /translation="QVIQAELALGRTIYGYGRPLAKLDERIPHTLALARELGLAEGKH
                     LQLALAVYRYLKQSKGLSMNIAAIDAALAADIGLSPEEYQLFMSPCFIAGMTPCYLDG
                     RDKPEGAFFPMRCSSVQYHGVARREWT"
BASE COUNT          370 a          283 c          223 g          375 t
ORIGIN      
        1 aagaacgtac aaactactat gcttttggcc aagatgaatg gcaaattgcc aatacacttc
       61 aattaacaac aggcttgcgt tatgaccatt tctccgattt tggttcaacc gttaatccgc
      121 gtttagctct tatttgggca acaacacccg actttaccac taagttatta tatggacgtg
      181 cctttcgcgc gccagccatt gccgaattat ttgttaccag taatcctgtt cagttaggta
      241 atcccgattt aaatcccgaa accattgata cctacgagct tgctttttct catcaagcct
      301 ctagtcagtt gttatacagt gccaatattt attactataa aattcgtgac tatattgttt
      361 ttgtaccaat tagccaattt gccaatcaaa cacaaaatag tggcaaacgc catggctatg
      421 ggttgagttt agaagctgat tactctatga cagataattt tcgtttagta ggtaactaca
      481 gttatcagca agcaaaagat gacaataccc atgaagcggc tggcgaaagc ccagaccatc
      541 aattgtatgc acgcggtgaa tggcaatttc ttccttcttg gctatttagt ccacaagtta
      601 atgtcattgg tcagcaaaaa cgtgccgcaa atgatgctcg tgatgctgtt gatgcttata
      661 caaccgttga ttttacctta cgcaaaaaaa ataatattca acctttatcc tttgctttgt
      721 ctgtacataa cgtatttaat gcagatgtaa gagagcctag ccttgcttct ggtgctattg
      781 tggatgatta ccctatggcc aaacgtagtg tgatggcaga attcaattat caattttaaa
      841 ccttaggtgt tattaacacc taagtccact cacgccgcgc aaccccatga tattgcacgc
      901 tactacaacg cattggaaaa aatgcccctt cgggtttatc tcggccatct aaataacatg
      961 gcgtcatgcc agcaataaaa cagggactca taaacaattg atactcttcg ggacttaagc
     1021 caatatctgc cgctaaggct gcatcaatag cggcaatatt cattgacaag cctttgcttt
     1081 gctttaaata acgataaacc gccaatgcta attgtaaatg tttaccctct gccaaaccca
     1141 actcgcgtgc caatgctaaa gtatgaggaa tacgttcatc taatttggcc aaaggtcgac
     1201 cataaccata aattgttcgc cctaaagcta attcagcctg tatcacttgt t
//
LOCUS       DAOQMB010000709         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96485_length_1250_cov_9.697908, whole genome shotgun sequence.
ACCESSION   DAOQMB010000709 DAOQMB010000000
VERSION     DAOQMB010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96485_length_1250_cov_9.697908"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..>1250)
                     /locus_tag="PLY05_13375"
     CDS             complement(86..>1250)
                     /locus_tag="PLY05_13375"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC04328.1"
                     /translation="QRISTMTVPPLLAKSSKLLPKPTRRILNNASDRLFRARDLVLAD
                     QTPYDVLHDNGLVKLRHYLPLTEDEILVDDTVTIINKKRHKVPLVIVPPLAVNMLIYD
                     LFPSRSLVKYYLAQGFDVYLIDWGMPTRKHTHYNLSTYVTEFMPEFLAKVREHSGQKE
                     LSLHGWSMGGIFTLCYTALTHDPDIRNLVILGTPINSHASGAIGKVYQFIERRAEWVR
                     KNTGFRIHNLNPQLLHTPGWANVVGFKMTNPVGSLMGYWELVVKLADRQFVINHATTS
                     AFLDKMVAYPGGIVQDMMVRIWIDNELAKGYMQLGKTEVRLADIQCSLLAGAGKSDNM
                     VTKAAVQTLMDHVSSTDKEFIVVAGGHMGILSGSKAPQDVWPKVGEWLAERSN"
BASE COUNT          373 a          265 c          249 g          363 t
ORIGIN      
        1 ccaatgttat tttaagtgtt gatggacgtt ggtgatacga gcggcagaat tccctctcct
       61 attaggagag ggctagggtg aggtcttaat tagacctttc agctaaccat tctcccactt
      121 ttggccaaac gtcttgtggg gctttgctac ccgacaaaat tcccatatga ccaccagcaa
      181 caacgataaa ttctttatct gttgaactca catgatccat taaagtttgt acggctgctt
      241 tagtgaccat attgtcgctt ttgccagcac ctgccaataa cgaacattga atatcagcca
      301 aacgtacttc ggttttgcct aattgcatat atcctttggc taattcatta tcaatccaaa
      361 tacgtaccat catgtcttga acaataccac ccggataagc caccattttg tctaagaaag
      421 cagaagttgt tgcatgatta atgacaaatt gacgatcagc caatttaaca accaattccc
      481 aatagcccat taaactgccc acaggattag tcattttaaa gccaaccaca ttcgcccaac
      541 ccggtgtatg cagtagttga ggattgaggt tgtgaatacg gaaacctgta ttttttcgta
      601 cccattcggc acgacgttca ataaattgat aaaccttacc aattgcacct gaagcatggc
      661 tgttgatggg tgtacctaaa atcactaagt tacgaatatc agggtcatgg gtgagtgcgg
      721 tataacataa agtaaaaatg ccacccatgc tccaaccatg taaagatagc tctttttggc
      781 cgctatgctc acgaacttta gccaaaaatt ctggcataaa ctcagtgaca taggtgctta
      841 aattgtagtg agtatgtttg cgcgttggca taccccaatc aattaaatac acatcaaaac
      901 cttgggctaa gtaatatttt accaagctac gactcggaaa taggtcgtaa atcaacatat
      961 tcaccgctaa aggcggcaca atgactaagg ggactttgtg gcgttttttg ttgataatgg
     1021 ttactgtgtc atcaactaaa atttcgtctt cggttaaggg taagtagtga cgtaacttta
     1081 ccaaaccatt atcgtgtaac acatcataag gtgtttgatc ggccaagact aaatcacgag
     1141 cacgaaacaa acgatcactg gcattgttta aaatgcggcg tgtgggtttt ggcaagagtt
     1201 tgctagattt ggctaataaa ggtggtacag tcattgtgct aattctctga
//
LOCUS       DAOQMB010000710         1250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96490_length_1250_cov_5.750628, whole genome shotgun sequence.
ACCESSION   DAOQMB010000710 DAOQMB010000000
VERSION     DAOQMB010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96490_length_1250_cov_5.750628"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLY05_13380"
     CDS             <1..330
                     /locus_tag="PLY05_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04329.1"
                     /translation="SNNFGLGMAGTNGRSGEAKAVVRGAIDTSSLSDEEVAAKNLAAE
                     KHAAAMQQAAVAAEKRMATKRESVIAVIGDNWSNRSKRMAQAKQLVSAGVFDETCSAM
                     EWLMTNH"
     gene            366..731
                     /locus_tag="PLY05_13385"
     CDS             366..731
                     /locus_tag="PLY05_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04330.1"
                     /translation="MINLTQHNATQDQLAVGVVDLTGQQLALVKALLTFDEIPTEFDM
                     KERADKIAEIAKKHGVAAMIGGAPFFMSYLERALHKAGVSPYYAFSKRCVIKDEDGIK
                     ERVIFKHEGFVDAFWDGNY"
     gene            731..988
                     /locus_tag="PLY05_13390"
     CDS             731..988
                     /locus_tag="PLY05_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04331.1"
                     /translation="MRSPSPLRVEGRKALERGLKRFVPSDTDYRCKLHGSDTNEHYSS
                     SGRCCLCDTKQKKPEQQAAYWQTVKDEWSKKRKEAYKRKAL"
BASE COUNT          395 a          244 c          292 g          319 t
ORIGIN      
        1 agcaacaatt ttggtttggg catggctgga acaaacggtc gctctggcga ggcgaaagcc
       61 gtagtgcgcg gagcaattga cacctcttct ctttcagatg aagaggtagc tgcaaaaaac
      121 ctagctgcgg agaaacacgc ggcagctatg caacaagcgg ctgttgctgc ggaaaaacgc
      181 atggcgacaa aacgcgaatc cgttatcgct gtcattggcg acaattggag caatcgttct
      241 aaaagaatgg cacaagccaa gcaattggta agtgctggcg tatttgatga aacctgctcc
      301 gctatggagt ggttaatgac aaaccactaa aaaaccatta gtaacataat tttttggaga
      361 aaaacatgat caatttaaca caacataatg caacacaaga ccagttagcc gttggcgtag
      421 ttgacttaac aggccaacaa ttggctttag taaaggctct tttgaccttt gatgaaatcc
      481 ctaccgaatt tgacatgaag gagcgagccg acaaaatcgc tgagattgca aaaaagcatg
      541 gtgtagcagc catgattggt ggcgcaccgt tttttatgag ctatttagaa cgcgccttac
      601 acaaagctgg tgtaagccct tattatgctt tcagcaagcg ttgcgtcatt aaggatgaag
      661 atgggataaa ggagcgcgtt atcttcaaac atgagggttt tgttgatgct ttttgggatg
      721 ggaattacta atgagaagtc caagccctct aagagtcgag ggcagaaaag ccctagagcg
      781 tggcttaaaa cgctttgtgc caagcgacac ggattatcgt tgtaaattgc atggcagcga
      841 cactaacgaa cattacagtt caagtggtcg ctgttgcttg tgtgatacca agcaaaaaaa
      901 gcccgaacaa caggcggctt attggcagac agtaaaagat gaatggtcta agaaacgcaa
      961 ggaagcgtat aaaagaaaag ccctttaact ggggcttttt atgtctaaag tttgtctaaa
     1021 atcaaataaa ctaaatctgc tgaaaactta caagaaaaag acttagctat tatcacggcc
     1081 aaaggtgatt caatgtcgcc tactatcaaa gtgatagtcg catcatttta gtatttgact
     1141 atttgcagtt ttgcagtagt atttagctaa tctagtcgta ttctgatttc aataaaggtc
     1201 gcccataaag cggccttttt tattgcctaa aataatcctc cgcacaagga
//
LOCUS       DAOQMB010000711         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96712_length_1249_cov_3.235343, whole genome shotgun sequence.
ACCESSION   DAOQMB010000711 DAOQMB010000000
VERSION     DAOQMB010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96712_length_1249_cov_3.235343"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..426)
                     /locus_tag="PLY05_13395"
     CDS             complement(238..426)
                     /locus_tag="PLY05_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04332.1"
                     /translation="MTVATEAGTLKVALPVVPVVVSPSGALLAAFFNLTLAFKILPKD
                     GVTVSVLSVTALVLLASD"
     gene            401..>1249
                     /locus_tag="PLY05_13400"
     CDS             401..>1249
                     /locus_tag="PLY05_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HNC04333.1"
                     /translation="MPASVATVIAEVQGGKGAKYFDEAKKQMVDMPATTVIRAAASVV
                     NTNSEIGVTALTEAAVQRAEALAGTGGNIIPHLNTAKATVESVFGVQDILKAPALVGS
                     TLDMANLGSSQAEQYALRLASLAKIAAQNLGSGEAAPAAKMAQAIALDLADGDIDGSG
                     NTGTLPYDLATFAAQYQAQMITLVQDLLANASQGGFDATKLQALLTFVQNNPVSLNVT
                     PPPIPFALTGFTQVSAAVGAEVTITGTGFVADPTKMKVTFANNVMAEIISATANSLVV
                     KVPQGAV"
BASE COUNT          404 a          243 c          265 g          337 t
ORIGIN      
        1 gcaatagtac ataaaaagag cgataaccac aaaataatta agttttttgt ataacatata
       61 ttgatgtact agctataaat agcctaaaaa ttaatgcaag aaagctaaaa acttaaatta
      121 tgttgtcaat tcaaaggaga ataaccatga caaacactaa aaaattcata ttgacgccta
      181 tagttacagc aatcttgttg agtggttgta atagtaatga tgacgatcca gttgctgcta
      241 atcacttgcc aataatacca atgctgtaac gcttaaaacg cttactgtca cgccatcttt
      301 aggcaaaatt ttaaacgcta aagttaaatt aaaaaatgct gctaataatg cgccacttgg
      361 tgaaacaaca acaggcacaa caggtaaagc cacttttaat gtgcctgctt ctgttgctac
      421 cgtcattgcc gaagtgcagg ggggcaaagg tgcaaaatat tttgatgaag ctaaaaaaca
      481 aatggtggat atgcctgcaa caacggttat tcgtgccgca gcctcagtcg tcaataccaa
      541 tagtgaaatt ggcgttacgg cgttaactga ggcagccgta caacgtgcag aggcattagc
      601 aggaacaggc ggtaacatta ttccacattt aaacacggct aaagcaaccg tagaatctgt
      661 ttttggtgta caagatattt taaaagctcc agcgttagta ggcagcactt tagatatggc
      721 caatttgggc agtagccaag cagagcaata cgccttacgt ttggccagtt tagctaaaat
      781 tgctgcgcaa aacttaggta gtggcgaggc agctcctgct gccaaaatgg cacaagcgat
      841 tgctttagat ttagccgatg gtgatattga tggttcgggc aatacaggca cattgcccta
      901 tgacttggca acttttgccg cacaatatca agcacaaatg attactttag tacaagatct
      961 attagctaat gcttctcaag gcggttttga tgcaaccaag ttacaagcct tgttgacgtt
     1021 tgttcagaat aatcctgtta gccttaatgt gacaccgccc cccattcctt ttgcattaac
     1081 aggatttaca caagtgagtg ctgctgttgg tgcagaggta acgattacag gcacaggttt
     1141 tgtagcagac ccaactaaaa tgaaagtcac ttttgcaaat aatgtgatgg cagagattat
     1201 cagtgcaacg gcaaactcat tggttgtgaa agtgcctcaa ggtgcagtg
//
LOCUS       DAOQMB010000712         1249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96762_length_1249_cov_2.515075, whole genome shotgun sequence.
ACCESSION   DAOQMB010000712 DAOQMB010000000
VERSION     DAOQMB010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96762_length_1249_cov_2.515075"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..>1249
                     /locus_tag="PLY05_13405"
     CDS             424..>1249
                     /locus_tag="PLY05_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein/DEAD/DEAH box
                     helicase"
                     /protein_id="HNC04334.1"
                     /translation="MDISQVKELGDIAEQKVLSALSSLPSPWQIFNTVEWRNIGNYGE
                     EVGEADVVVFNPQYGLIVFEIKAGNVYLKDGQWFYASGKPMKQSPLSQARRNRYALMK
                     RLELKLGTDVNKLNITHAVWFPDVRWQAAITLLEAPSINFIFDKTCLANPEPRLLKIF
                     FELSNNFVTWTKTQQRLLKELLAPDCHLLVPLASKIDDTVHQLLTATEQQINILRLLR
                     HQQRLLVEGGAGSGKTLLAVTLAREHALLGKDVLLPCFNKALALQLKLSLADYPNIT"
BASE COUNT          411 a          196 c          255 g          387 t
ORIGIN      
        1 gaggctataa ggtatggaag atatgagtgt gatgtactca ggtgagtaat atatatttga
       61 atttttttgg gataagggtt aggtaggtaa gattagacaa cacctgtcta agttaagcga
      121 tatgatgact taatatgaat aggccatggt taaatatata ttaatcatta tcttaaatta
      181 tatcagcttt tatgctaacc aaagttggcg tataaattta actggtttag aagcccaaca
      241 tcccctgtca catcaacaca aacgacagaa gtaaaacatt tatatcaacg atagatagcc
      301 ttccttcgag tatggtaaaa gcgtgttaat gataatatta agggcgtttg aatgttgcaa
      361 atagacggca ttattggcgc atctagaaga atattttgat tttaatgaga gacattactt
      421 aaaatggata ttagtcaagt taaagagcta ggggatattg cagagcaaaa agtattgtca
      481 gccttgtcta gtttaccctc gccatggcag atatttaata ccgtagaatg gcgtaatatt
      541 ggcaactatg gtgaggaagt tggtgaggct gacgttgtgg tttttaatcc acagtatgga
      601 ttaattgttt ttgaaataaa agcagggaat gtttatctta aagatgggca atggttttat
      661 gcttctggta agccaatgaa gcagtcacca ttatcgcaag ctagacgaaa tcgttatgct
      721 ctgatgaaac ggcttgaact caagttggga acagatgtta ataaattgaa tatcacacat
      781 gctgtttggt ttcccgatgt ccgttggcaa gcagctataa cattattaga agctccttcc
      841 attaacttta tttttgataa aacctgtttg gcaaatccag agccacgttt actaaaaatt
      901 tttttcgagt tatcaaataa ttttgtgaca tggacaaaaa cacaacaacg tttgctaaaa
      961 gagttgttag cacctgactg ccatttatta gtgccattag cctcaaaaat agatgacaca
     1021 gttcatcaat tattgacggc tacagaacag caaataaata tactacgttt attaaggcat
     1081 caacagcgac tattagttga aggtggggca ggttcaggta aaacattatt agcggtaact
     1141 ttagcacgcg aacatgcttt attaggcaaa gacgtattat tgccctgttt taataaagca
     1201 ctagcactcc aacttaaatt gtcgttggcg gattacccta atatcaccg
//
LOCUS       DAOQMB010000713         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96821_length_1248_cov_12.379715, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000713 DAOQMB010000000
VERSION     DAOQMB010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96821_length_1248_cov_12.379715"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..993)
                     /locus_tag="PLY05_13410"
     CDS             complement(28..993)
                     /locus_tag="PLY05_13410"
                     /inference="COORDINATES: protein motif:HMM:NF014601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC04335.1"
                     /translation="MKEHILIIGAGAVGQVYAYHLAQAGYHIHFLLKEKYLEEAQQGF
                     VLYNLRQDKQRQQAIIFNNFSCHTSWQSLTNIKLSQVWLCVSSTALAAMDLAPMKAAI
                     GEALVVVLQPDPDDVTRVQQTFGKEQVAAGMINMISYHTPLATEQTAREGIAFWIPPV
                     MPMPVDGEVEGVSALLAVLKKAKISALWQKQFAIANAHNTGFLMVFLAALELSRWQFK
                     QLSANKALLKTMIAAQQEVFAALQAEYGSKASRVLRQIKPWMLPVILKLARHVAPLDM
                     ETYLQAHFMKVRSQTLLLLDAYVQRANKSGIAVPNLQGLVDKLDR"
BASE COUNT          378 a          282 c          225 g          363 t
ORIGIN      
        1 gtgggtttcg tacctcaacc cacgctacta tctatccaac ttatccacca acccttgcaa
       61 atttggaaca gcaataccac ttttattagc tcgttgaaca taagcgtcta acaacaataa
      121 ggtttgtgag cgtactttca taaagtgcgc ttgtaagtag gtttccatat ctaagggagc
      181 aacatgacgt gccaatttta ggatgactgg taacatccac ggttttattt gtcgcaacac
      241 cctactggct ttactaccat attctgcctg taaggcagca aatacttctt gttgtgcggc
      301 tatcatcgtt tttaataacg ctttatttgc tgataattgc ttaaattgcc aacgacttaa
      361 ttctaaagcg gccaaaaaca ccatcaaaaa gcctgtattg tgggcattag caatcgcaaa
      421 ttgtttttgc cacaaggcac taattttggc tttctttaac acggccaaca atgccgacac
      481 cccttcaacc tcaccatcaa ctggcatagg catcacaggg ggaatccaaa aggcaatgcc
      541 ttctcgcgct gtttgctcgg tggctaaggg ggtgtgataa ctaatcatgt taatcatccc
      601 tgccgcgact tgctctttgc caaaggtctg ttgcacacga gtcacatcat cagggtcagg
      661 ttgcaaaact accactaaag cctcaccaat cgcggctttc atcggggcta aatccatggc
      721 tgccaaagcg gtgctggata cacacagcca gacttgacta agcttaatat tggtcaaact
      781 ttgccatgag gtgtgacaac taaaattatt aaaaataatc gcttgctgac gctgtttgtc
      841 ttggcgtaag ttatacaaaa caaaaccttg ttgtgcttct tctaggtatt tttcttttag
      901 caaaaaatga atgtgataac ccgcttgtgc caaatgatag gcatacacct gcccaactgc
      961 gccagcacca ataattaaaa tatgttcttt cattgttata atcaactcag cattagttcg
     1021 acacccatca aattattaca tatttcgata gatgtcaaac taaaaatcat caaaagctga
     1081 tatatgcagg gctgcgcatt tcaccgaaat taacgcgttt tgtaagcatt aaatcgttga
     1141 aaacgattaa aatgcttaca aataagcgat aatcgcgtca gtcctaaaaa taatcatatt
     1201 tatatgagag gataaaccta atgcctacta tctttgagaa aatcattg
//
LOCUS       DAOQMB010000714         1248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_96825_length_1248_cov_7.231350, whole genome shotgun sequence.
ACCESSION   DAOQMB010000714 DAOQMB010000000
VERSION     DAOQMB010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96825_length_1248_cov_7.231350"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="zapE"
                     /locus_tag="PLY05_13415"
     CDS             <1..680
                     /gene="zapE"
                     /locus_tag="PLY05_13415"
                     /inference="COORDINATES: protein motif:HMM:NF015902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HNC04336.1"
                     /translation="ITDAMLLKGLFEQLFARGVSLVATSNIVPDRLYENGLQRERFLP
                     AITLVKDNCQVLNVDSGIDYRLRVLTHVELYHYPLGDIAQKTLQKSFDELAHGTHVHH
                     DSIIINDRDVMVKARTEDVLWCDYHELCEKPRSASDFIEIAREYHTILLSNVPVMGEK
                     NDDSTRRFINLIDELYDRQVKLIITAEASIDNIYSNGRLNFEIKRTRSRLQEMQSQEY
                     LQLPHLP"
     gene            768..>1248
                     /locus_tag="PLY05_13420"
     CDS             768..>1248
                     /locus_tag="PLY05_13420"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HNC04337.1"
                     /translation="MSYEQQQIKAIQLWQGQGPKLTSMLAGMVSAPAVKLVDMVIPDS
                     AIQAALEAACSVGEKLTNPQSVLEQANVSQIGDLFYAKLQLCDELANKTHDRAIAMAA
                     AEGGVTGASGLVGVAIDIPTLMTLALRTIYQTALCYGFELKGEEGRQVALRVFSIASA
                     "
BASE COUNT          352 a          232 c          293 g          371 t
ORIGIN      
        1 atattaccga tgccatgtta ttaaaaggtt tatttgagca attgtttgcc cgtggcgtca
       61 gtttggtggc caccagtaat attgtgcctg atcgtttata tgaaaatggc ttacaacgag
      121 aacgtttttt gcctgcgatt actttggtta aagataattg tcaggtatta aatgttgatt
      181 cgggcattga ttatcgtttg cgagtcttaa cccatgtgga attgtatcat tatcctttag
      241 gcgatattgc ccaaaagacc ttgcaaaaaa gttttgatga attagcccat ggcacacatg
      301 tacatcatga tagcattatt attaatgatc gtgatgttat ggtcaaagcg cgtactgaag
      361 atgtgttgtg gtgcgattat catgagttgt gtgaaaagcc gcgtagtgcg tcagacttta
      421 ttgagattgc ccgtgaatat cacacaattt tattaagcaa cgtgccagtg atgggcgaaa
      481 agaacgatga ctccacacga cgttttatta atttgattga tgagttatac gatcgccaag
      541 taaagttgat tattacggcg gaagcaagta ttgataacat ttatagcaat ggtcgtctaa
      601 actttgaaat aaagcgcaca cgcagccgtt tacaagaaat gcagtcgcaa gaatatttgc
      661 agttacccca tttgccataa acacattgat gtttgttttt tccttcgact ccgctcagga
      721 atcagcttat gctggctgag cggagtcgaa gcccagtgga gatagttatg tcctacgaac
      781 agcaacaaat taaagctatt caactttggc aaggacaagg gccaaaactc acctcgatgt
      841 tagcaggaat ggtttctgcc cctgcggtta aattagtcga tatggtcatt cccgactcag
      901 cgattcaggc ggctttagaa gccgcttgta gtgttggcga aaaactcacc aatccacaaa
      961 gtgttttaga gcaagcaaat gttagtcaaa ttggtgattt attttatgca aagctacagt
     1021 tgtgtgatga gttagccaac aaaacccacg atcgagccat tgccatggct gcggcagaag
     1081 gtggggtaac aggcgcgagt ggtctagtgg gagtagcgat agatattccc accctaatga
     1141 ctttggcctt acgcaccatt tatcaaacgg ctttatgtta tggttttgag cttaaaggtg
     1201 aagaagggcg gcaagtcgct ttacgtgtat ttagtattgc cagtgcca
//
LOCUS       DAOQMB010000715         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_97154_length_1246_cov_11.229219, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000715 DAOQMB010000000
VERSION     DAOQMB010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97154_length_1246_cov_11.229219"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /gene="folE"
                     /locus_tag="PLY05_13425"
     CDS             complement(<1..436)
                     /gene="folE"
                     /locus_tag="PLY05_13425"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HNC04338.1"
                     /translation="METMQNAYAQIIASVGEDLNRAGLLDTPKRAAKAFQYLCKGYED
                     DLNTIVNNAVFPSDNDEMVIVKDIELYSLCEHHLLPFIGKCHIAYLPSGNVLGLSKFA
                     RIVDMFARRLQIQENLTKQIADAVQQVTGAKGVAVIIEAKHMC"
     gene            complement(657..>1246)
                     /locus_tag="PLY05_13430"
     CDS             complement(657..>1246)
                     /locus_tag="PLY05_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNC04339.1"
                     /translation="ITRKYGGTGLGLAISKQLIDLMRGSINVQSTLHKGTTFVILLPV
                     SIATEPPMTTSPMLPALAWGDFSQLRVLLVEDNSINQLIISSLLNELRIEPQLANNGA
                     EAIELISQHHEHLDVVLMDCEMPILDGLEATRKIREWEKVHAKPHLPIIALTAHALPE
                     YKERCLAVGMTDYLSKPLLLEQLMNKLLNIQKNSS"
BASE COUNT          395 a          242 c          235 g          374 t
ORIGIN      
        1 tgcacatgtg tttggcttca ataattacgg cgacgccttt cgcgcctgtt acttgttgta
       61 cggcatcagc aatttgtttg gttaagttct cttgaatctg caaacgacga gcaaacatat
      121 cgacaatacg cgcaaatttg gataaaccta aaacattacc actaggtaaa taggcaatgt
      181 ggcacttacc aataaaaggc aacaaatgat gctcacacaa agaatacagc tcaatatctt
      241 taacaatgac catttcatcg ttatcagagg gaaatacagc attattaacg atggtattta
      301 gatcgtcctc ataaccttta cacaaatatt gaaaggcttt tgcggcacgt ttaggggtat
      361 caagtaaacc tgcacgattt aaatcttctc ctacagacgc aatgatttgt gcataggcgt
      421 tttgcatggt ttccataata ataactcggg tgttttagag agtttagatt aggctaaact
      481 cgatgggtga aaacacaaca tgatgtttta tcaaacctca tattgcaaaa agagtctgca
      541 ccataaccta agttaagcca aaggtaaacg taacagaggc tatctaaagc tgctatttag
      601 atgattttgt tctttgttta tgctttgtat aaacaaaaaa atgacgccaa atagatttat
      661 gagctatttt tttgaatgtt tagaagtttg ttcattaact gttctagtaa aagaggctta
      721 cttaaatagt ctgtcatgcc aaccgctaaa caacgttctt tatattcagg taaagcatgt
      781 gcagtcagcg caataatggg taaatgaggc tttgcatgga ctttttccca ttcacgaatt
      841 ttacgcgtag cttcaaggcc atcgagtatg ggcatctcac aatccattaa gacaacatct
      901 aaatgctcat gatgttggct aatcagttca atcgcttctg caccattatt ggcaagttgt
      961 ggttcaatgc gtaattcatt caacaacgaa ctaataatta actggttaat cgagttatct
     1021 tcaaccaaca gcacacgcaa ctgcgaaaaa tctccccatg caagtgcagg taacattggg
     1081 ctagttgtca tcggaggttc tgttgcaatc gaaacgggca ataaaataac aaatgtcgtc
     1141 cctttgtgta gggtgctttg cacattaata gagccgcgca ttaaatcaat caattgtttg
     1201 ctaatggcta aacctaaacc tgtgccgcca tatttacgag taatag
//
LOCUS       DAOQMB010000716         1244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_97453_length_1244_cov_8.729184, whole genome shotgun sequence.
ACCESSION   DAOQMB010000716 DAOQMB010000000
VERSION     DAOQMB010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97453_length_1244_cov_8.729184"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="mreC"
                     /locus_tag="PLY05_13435"
     CDS             complement(<1..490)
                     /gene="mreC"
                     /locus_tag="PLY05_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNC04340.1"
                     /translation="MSIPIERQKRRFNILATHSYRLLLVVMMGAGIFWLDSRYPMVVE
                     PVHHYGQQVIEPFIRLLSYPQRIIIAIQQELRSPQDLQQENKLLSRVITQQAVELQQL
                     AELKAENDRLRALINMQARLDENLVNAEVISSAVQLEQQVLMINKGLREGLQVGYPVL
                     DAK"
     gene            complement(499..>1244)
                     /gene="mreB"
                     /locus_tag="PLY05_13440"
     CDS             complement(499..>1244)
                     /gene="mreB"
                     /locus_tag="PLY05_13440"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HNC04341.1"
                     /translation="KKNMRVLVSVPAKATLLEHRAVREAVDGAGANDIRLIHQAMAAA
                     IGAGLPIDQACGSMVVNIGAGTTEIAVISLLGSVYTDSLRVGGDTINERITTYIRRKY
                     GCLIGEASAEHIKQEIGIAIVNSDSEQQNTMEVRGRHLSAGVPTAIKVSAAEIAEVLQ
                     EPLRNIINAIKNALEDMPAELSGDIAERGIVLTGGTARLKHLDKLFSQEIGLPMILAD
                     EPENCVIRGMGQALSFLDQPAYDSLFVYS"
BASE COUNT          371 a          287 c          224 g          362 t
ORIGIN      
        1 cttttgcatc taaaacggga taaccaacct gtaaaccttc tcttaaacct ttatttatca
       61 tcaatacttg ctgttctaat tgtactgctg atgaaatcac ttcggcattg actaagtttt
      121 catccaaacg tgcttgcata ttaattaaag cacgtaaacg gtcattttct gcttttaact
      181 ccgcaagttg ttgtaactca actgcttgtt gagtaattac ccgtgataat aatttattct
      241 cttgttgcaa gtcttgtggg ctacgcaact cttgttgaat tgcgatgatg atgcgttgag
      301 ggtaactcaa caaacggatg aaaggctcga tcacttgttg accataatga tgaacaggct
      361 caacaaccat aggataacgg ctatccaacc aaaagattcc tgctcccatc attacaacta
      421 acaataagcg gtaagaatgt gtggctaaaa tattaaaacg acgcttttgt cgctcaatag
      481 ggatagacat acatgttctt aggagtaaac aaagaggctg tcataggcag gctgatcaag
      541 aaatgataat gcttgtccca taccacgaat cacacaattt tctggctcgt ctgctaaaat
      601 catgggcaag ccaatttctt gactaaataa tttatctaga tgttttaatc tggctgtccc
      661 acctgttaac acaatacctc gctcagcaat atcacctgac aactcagcag gcatatcttc
      721 taaagcattt ttaatggcgt taataatatt gcgcaacggc tcttgtaaca cttcagcaat
      781 ttctgcggca ctgactttta ttgccgttgg cacacctgcg gacaaatgac gccctctgac
      841 ttccatcgta ttttgttgct cagaatcaga attgacaatc gcaataccga tctcttgttt
      901 aatatgctct gcagaagctt caccaattaa acaaccatat ttacggcgaa tataggtggt
      961 aattctttcg ttgattgtgt caccaccaac tcgcaaagaa tccgtataca cagagcctaa
     1021 caaggaaata accgcaattt cggttgttcc tgcaccaata ttgacaacca ttgagccaca
     1081 ggcttgatcg atgggtaaac ccgcaccaat ggctgccgcc atcgcttgat gaattaaacg
     1141 gatgtcatta gcaccagcac cgtctacggc ttcacgcaca gcacgatgct ctaataaagt
     1201 ggctttggca ggtacactaa ccaaaacacg catattcttt ttaa
//
LOCUS       DAOQMB010000717         1243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_97602_length_1243_cov_16.257576, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000717 DAOQMB010000000
VERSION     DAOQMB010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97602_length_1243_cov_16.257576"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..1168
                     /locus_tag="PLY05_13445"
     CDS             281..1168
                     /locus_tag="PLY05_13445"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HNC04342.1"
                     /translation="MTLTDLRYLVTLAELRHFAKAAAACFVSQPTLSIAIKKLEDELN
                     LVLFERHRHDVLITPAAEPIIAQAKVVLHEADLLKQMAQAQRDEFAQPLRLGAIFTVA
                     PYVFPSLIPALHQSAPQLLLYLEENYTHVLSDKLANGELDVILVAAPFELPECQSADL
                     WQEEFLLLMPQKHPWVSETHIDSHLLADAPMLMLGEGHCFRNQSLATCPMNNARLNNG
                     NSLETLRFMVANGLGLTLIPAMSVPYLVNQSLSYKSLNPAPTRQIMAVWRRRFPRRQA
                     IYALIQGLKQLKLAGTQSL"
BASE COUNT          368 a          283 c          243 g          349 t
ORIGIN      
        1 ttaatcaccg ttgcacctgc caactctaaa ggtgaacgtt tgataatcgt tagctcatga
       61 ccaatactga ttagcttgtt agccaaacgt aaacccacat caccacagcc aattaataat
      121 atatttgcca tctttactat tcactcataa actaaaacaa tcaatattta aaatttaatt
      181 aatttttccc tcaccccaac cctctcccaa agggcgaggg agttctctct tcccgaggga
      241 gcgagttaga gtgagggttc agagtctaaa gtacaatata atgaccctaa ccgacctacg
      301 ctatttagtc actcttgccg aattgcgcca ttttgccaaa gccgcagccg cgtgttttgt
      361 ctcgcaaccg acactgagta ttgccatcaa aaaactcgaa gatgaactta atctcgtgtt
      421 atttgagcga catcgccatg atgtactgat tacgcctgca gcagagccta tcattgctca
      481 ggccaaagtg gtactgcacg aagcggattt acttaaacaa atggcacaag cgcaacgtga
      541 tgaatttgcc caacctttac gtttaggtgc gatttttact gttgccccct atgtgtttcc
      601 cagtttaatt cctgccttac atcaaagtgc gccacaatta ttattgtatt tagaagaaaa
      661 ctatacccac gttttaagtg acaaattggc caatggcgaa ttagatgtga ttttagtcgc
      721 tgcacccttt gaattgcccg aatgccaaag tgcggatttg tggcaagagg agtttttatt
      781 attaatgcca caaaaacatc cgtgggtaag cgaaacacac attgatagtc atctgctcgc
      841 tgatgcaccg atgttgatgt taggtgaagg tcattgtttt agaaaccaaa gtttagcaac
      901 ttgcccgatg aacaatgcca ggctcaataa tggcaactct ttagaaacgc tacgctttat
      961 ggtggccaat ggtttgggct taacactgat tcctgcgatg agtgtgcctt atttagtcaa
     1021 tcaaagttta agttataaaa gcctaaatcc tgcacccacg cgacaaatta tggctgtttg
     1081 gcgtagacgt tttccacgac ggcaggcaat ttatgcttta atacaaggac ttaaacaatt
     1141 aaaacttgca ggaacacaga gcctatgagc caacatttag cacatatatc cgtacaacag
     1201 cttaaaggcg tgggcacgat gctcgccgat aagttagcca aat
//
LOCUS       DAOQMB010000718         1242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_97844_length_1242_cov_2.846672, whole genome shotgun sequence.
ACCESSION   DAOQMB010000718 DAOQMB010000000
VERSION     DAOQMB010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97844_length_1242_cov_2.846672"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..>1242)
                     /locus_tag="PLY05_13450"
     CDS             complement(116..>1242)
                     /locus_tag="PLY05_13450"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04343.1"
                     /translation="SLWQGEIYRHPKIKIAYLSADFYQHATSILMIELFEQHDRSQFE
                     IIALAFNTQNDDMTTRIKKAFDKYIDVSKFTDLQIAQLIKSLEIDIAVDLKGHTQDAR
                     LGIFAHKAAPIQVNYLGQASTIGADYIDYILADKCVVPPDEQPFFSEKVVYLPHTYYV
                     NDSQRPISAQLVSRDELGLPAQGFVFCCFNNSYKISPMMFDVWMSLLQQVPDSVLWLL
                     EANSTARENLCNETKKRGIALSRLVFAPKVASSLHLARHQHADLFLDTLPINAHTTAC
                     DALWARVPVLTCIGQTFASRVAASVLTAVGLPELITQNLVEYQALALKLATSPLLLAQ
                     VKQKLQHRELSPLFDIKIQCKAFESAYHTMYARYQQGMTP"
BASE COUNT          375 a          250 c          226 g          391 t
ORIGIN      
        1 ggtatggagc aatcatcgct gatttgtttt tctaaagttt ttctaaaaat acgctaatta
       61 ttacaaacta ttactgattt aatttttttc atcttagggt ctatttatac gttgattatg
      121 gtgtcatgcc ttgctgataa cgtgcataca tcgtgtgata agccgactca aatgccttac
      181 attgtatttt aatatcaaac aacggactta gttcgcgatg ttgtaatttt tgttttactt
      241 gggctaatag taacggcgat gtggctaatt ttaatgctaa ggcttggtac tctaccaaat
      301 tttgggtaat taactcaggc aaaccaaccg ccgtcaaaac acttgccgct actctgcttg
      361 caaatgtttg ccctatacag gtcagcacag gcacacgcgc ccataatgca tcacatgcgg
      421 tggtatgcgc gttaattggc aaagtatcta aaaataaatc agcgtgttgg tgacgtgcca
      481 aatgcaaact tgaggctact ttaggcgcaa aaaccaatcg acttagcgca ataccacgtt
      541 tttttgtttc attacacaaa ttctctctgg cggtactatt cgcctccaac aaccataaca
      601 cgctatctgg cacttgctgt aacaagctca tccaaacgtc aaacatcatg ggcgaaattt
      661 tgtagctatt attaaaacaa caaaatacaa aaccttgtgc tggcaaaccc aattcatcac
      721 ggctaacaag ctgtgcagat ataggacgtt gggagtcatt aacataataa gtatgcggta
      781 aatacaccac tttttcacta aaaaatggct gttcatcagg tggcacaaca cacttatcag
      841 ccaaaatata atctatataa tctgcaccaa tcgtactagc ttgccccaga taatttacct
      901 gtattggtgc tgctttgtga gcaaaaatcc ctaaacgtgc atcctgagta tgtcccttta
      961 aatcgacagc gatatcaatt tctaatgatt ttatgagctg ggcaatttgt aagtctgtga
     1021 atttactaac atctatatat ttatcaaagg cttttttaat gcgggtggtc atgtcgtcat
     1081 tttgagtatt aaacgcaagt gcgattatct caaactgtga acggtcatgt tgttcaaata
     1141 actcaatcat taaaattgat gttgcatgtt gataaaagtc agccgaaaga tacgctattt
     1201 ttattttagg atggcgatat atttcgccct gccacaaaga gg
//
LOCUS       DAOQMB010000719         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_98103_length_1240_cov_11.256540, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000719 DAOQMB010000000
VERSION     DAOQMB010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98103_length_1240_cov_11.256540"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1240)
                     /locus_tag="PLY05_13455"
     CDS             complement(<1..>1240)
                     /locus_tag="PLY05_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04344.1"
                     /translation="EDEYLPYLITEQAKVQQATPQQRNAVQRLIDHIVRAVKGWVVRT
                     LSKYDTTYKMSRRVAESLTPADILYMAERMVGEVAKYEKPISPNKQSGMARREFLGLL
                     GAGVGVAALGGGATLIDQSIKNNTKEAAKEKVNSLADNVYNELIKQLNKSDAFVKLAK
                     NIAKAKPSNIGKNAAINGFYSITDTYRMEKMYANNEKSNLIDTGLRYKDDGTPIFVSR
                     DDVLKKASDTHALLSENEQKIIAIMSDAIGFVEAITGHNFKKLDIKATTNTPVADILA
                     RKKLGLKNHDTNINALVENNNPNVVLIHPQTMANLVSTNEKARLHAFQTLSHELVHVL
                     QNQENGVASNKGIGVNIDTPVLLKNMISNILGNEYSSSEQIQNETEAFHVGYLATKAL
                     LLKEGKPLSTISKDEFLNII"
BASE COUNT          328 a          253 c          209 g          450 t
ORIGIN      
        1 ttgattatat tcaaaaactc atctttgcta attgttgata atggcttacc ttcttttagc
       61 aacaatgcct ttgttgccaa ataaccaaca tgaaaagcct cagtctcatt ttgtatctgt
      121 tcgctagagc tatattcatt tcccaatata ttgctaatca tgtttttgag taaaacaggc
      181 gtatcaatgt ttacaccaat acctttatta gaagccacgc cgttttcttg gttttgcaaa
      241 acgtgtacta attcatggct taaagtttgg aacgcatgaa gccttgcttt ttcattggtt
      301 gatacaagat tagccatcgt ttgtggatga attaacacaa cattaggatt gttattctca
      361 actagcgcat tgatattagt atcgtggttt tttaatccaa gtttttttct tgccaaaata
      421 tcagcaacag gggtattagt tgttgcttta atatccagct tcttaaaatt atgcccagta
      481 atagcttcaa caaaaccaat agcatcagac ataatcgcta ttattttttg ctcattctct
      541 gataaaagcg catgagtatc tgatgctttt tttaacacat catctctact aacaaaaatt
      601 ggcgtgccat catctttgta gcgcaaacca gtgtcaatca gattgctttt ctcattgttg
      661 gcatacattt tctccatgcg ataagtgtca gtaatactgt aaaagccatt aatggctgca
      721 ttttttccaa tgtttgatgg ttttgctttt gctatatttt tagctaactt aacaaaagca
      781 tcactcttgt taagttgttt aataagctcg ttatacacat tatcggcaag agaatttacc
      841 ttttcttttg ccgcttcttt ggtgttgttt ttgattgatt ggtcaataag agtcgctcca
      901 ccacctaatg cagccacacc aacacctgcg ccaagcaaac ctaaaaactc tcgtcttgcc
      961 atgccgcttt gtttgtttgg gcttattggt ttttcgtatt tcgcaacctc acccaccatt
     1021 ctttcagcca tatacaaaat atcggcaggt gttaaagatt cagcaacacg cctgctcatt
     1081 ttgtatgttg tgtcgtactt gcttaatgta cgcactaccc agcctttaac ggcgcgtaca
     1141 atatggtcaa tcaagcgttg tacagcattt cgttgttgtg gtgtggcttg ctgtactttg
     1201 gcttgctcag tgattaagta aggtaagtat tcatcttcct
//
LOCUS       DAOQMB010000720         1236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_98753_length_1236_cov_4.111770, whole genome shotgun sequence.
ACCESSION   DAOQMB010000720 DAOQMB010000000
VERSION     DAOQMB010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98753_length_1236_cov_4.111770"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..559
                     /locus_tag="PLY05_13460"
     CDS             74..559
                     /locus_tag="PLY05_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04345.1"
                     /translation="MSVSPADLLAKNYKAAQALGQKEAQCDAYFEIEGFENLKFLAKT
                     FPRPILASAGVIESYTPNGVKVQQPQQLQVAQSHEVSFYATKGGAVEKALMQLNNNGG
                     TFQATVHLGQVDSPYASYQLTDCFLAEISPLDQDVEGVGQHVMISGTLYYHYFGERTE
                     S"
     gene            562..987
                     /locus_tag="PLY05_13465"
     CDS             562..987
                     /locus_tag="PLY05_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04346.1"
                     /translation="MTLTQLVAEFMASRLTGGLVLAESDVTKALVKAVRFYAGYAEIK
                     YFIGQVTPVLPTLIQIDSNVTLTPSEWAIIQPLFNAYADHENALRLEASRGLGLDVYG
                     RSSSELASEIKQLEMDLPRKAFYQSIASVGNLDIYADSN"
     gene            971..>1236
                     /locus_tag="PLY05_13470"
     CDS             971..>1236
                     /locus_tag="PLY05_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04347.1"
                     /translation="MLIQINDSAVIAQAFILSAILRTDLVPVPVSLELELKYDDSLAK
                     ELIEGTTLYVGSKAIPLQIVKSEIKKSLDNPNRGVIVIVALLAN"
BASE COUNT          379 a          236 c          262 g          359 t
ORIGIN      
        1 gtgtaactat caaaaaacac ctatcaaaac tcattcataa tagctttaac ttaaccgtca
       61 acgagatttt attatgtctg tttcaccagc cgatttatta gctaaaaact acaaggccgc
      121 tcaagcattg ggtcaaaaag aagcacaatg tgatgcttat tttgaaattg aaggctttga
      181 aaacttaaag tttttggcaa aaacattccc tagacccatt ttagccagtg caggtgtgat
      241 tgaatcttac acaccaaacg gtgtaaaagt gcaacaacca caacagctac aagttgctca
      301 aagtcacgaa gttagctttt atgcaaccaa aggcggtgca gtcgaaaaag ccttaatgca
      361 gttaaacaac aatggcggaa cattccaagc taccgtacat ttaggccaag tcgatagccc
      421 ttatgccagt tatcaattaa ctgattgctt tttagctgag attagcccac ttgaccaaga
      481 cgtagagggc gtaggccaac acgttatgat tagcggcaca ttgtattatc attattttgg
      541 tgagcgtaca gagtcttaac catgacctta actcaacttg ttgccgagtt tatggcctcc
      601 cgattaacag gggggcttgt acttgctgag tctgacgtaa caaaagcctt agttaaggct
      661 gtgcgttttt atgcaggcta tgccgagatt aagtatttta ttggccaagt aacacctgtt
      721 ttgcctacat taattcagat tgacagtaat gtgactttaa caccaagcga atgggcgatt
      781 attcaaccat tgtttaacgc ctatgccgac catgaaaacg cattgcgttt agaggcaagt
      841 cgtggcttag gtttagatgt ttacggacgc tcaagtagtg agttagcaag cgaaataaaa
      901 cagctagaga tggatttacc gcgtaaggct ttttatcaat ccattgcaag cgtaggcaat
      961 ttggatatat atgctgattc aaattaacga cagtgcagtc atagcacagg cttttatttt
     1021 gtcagcgatt ttgcgtactg acttagtgcc tgtgcctgtg tcgttagaac ttgaattaaa
     1081 atatgatgat agcttagcta aagaattgat agaggggact acactctatg tcggcagtaa
     1141 agcaatcccc ttgcagatag taaaatcaga aattaaaaag agcttagata atcctaatcg
     1201 tggcgtgatt gtgattgttg cgttgttggc gaattg
//
LOCUS       DAOQMB010000721         1235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_98881_length_1235_cov_9.871186, whole genome shotgun sequence.
ACCESSION   DAOQMB010000721 DAOQMB010000000
VERSION     DAOQMB010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98881_length_1235_cov_9.871186"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="PLY05_13475"
     CDS             <1..881
                     /locus_tag="PLY05_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04348.1"
                     /translation="MTETGVVPVDEYQPYEEPKPPKTSLITTSLGYEVVKEGELPRGA
                     PVVPTKEPKAPQLKEMTGLDGSANILNMATGEIKPVLVDGKPFITKDDPLTLFTEKEK
                     LKSELKQRETVAQKYSDASDVIPLLDGYIADLEKTPANFASSLYYKTKGLASTDNPEL
                     QAVYSANQKAKTLINYAQKQPGPSTDSDVRNYLEQVGVASDITVPRDARIAAAKSARK
                     YALSVYNKYEKYASDILSGKVTPDELKTEPSKPTRSKEELFRLYKEFKADYEAEKDPA
                     MRKIMIDAARADGLIK"
     gene            889..>1235
                     /locus_tag="PLY05_13480"
     CDS             889..>1235
                     /locus_tag="PLY05_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04349.1"
                     /translation="MGKYADLVAQYKQEQQTQKAKQLSAPLRGAVSAANSAMLNWGDE
                     LYGGLGALGAAVTGNDIGQAYQENRDLIRGIDTSFREEYPNTALGTQIAASLPATVYN
                     PLPLKTSNLALTGL"
BASE COUNT          416 a          242 c          272 g          305 t
ORIGIN      
        1 ccatgactga aacgggtgtt gtccctgttg acgagtatca gccttatgaa gagccgaaac
       61 cacctaaaac atcgcttatc acaacatcgc tagggtatga ggtggtaaaa gagggtgagt
      121 tacctagagg cgcgcctgtt gtacctacta aagagcctaa agcaccacaa ctcaaagaga
      181 tgacaggtct agacggttcg gcaaatatat taaacatggc gacaggtgaa ataaagcctg
      241 ttttagtcga tggtaagccg tttatcacaa aagatgaccc gctaacattg tttaccgaaa
      301 aagaaaaact taagtctgaa ttaaaacagc gtgaaacagt cgcgcaaaaa tactctgatg
      361 cttcggatgt tatcccattg ttagatggct atattgctga tttagaaaaa acacctgcta
      421 attttgctag ctctttgtat tacaagacaa aaggtttggc atcaacggat aatccagagt
      481 tgcaagcggt ttattcagca aaccaaaaag ctaaaacttt aataaactac gcgcaaaaac
      541 agcctggtcc atctacggat tccgatgtaa gaaattattt agagcaggtt ggtgttgcat
      601 cagatattac tgttcctcga gacgctagga ttgctgcagc taaatctgca agaaaatacg
      661 cattaagtgt ttataataaa tacgaaaaat atgcaagcga catattaagc ggcaaagtaa
      721 cgcctgatga gttaaagaca gaaccaagca aacctacacg atcaaaagaa gagttattta
      781 ggttgtataa agagtttaag gcggattacg aagcagaaaa agaccctgct atgcgtaaaa
      841 taatgataga cgcggctcgg gctgatggct taattaaata ggtgtaacat gggtaaatat
      901 gctgatttag tcgcacagta caagcaagaa cagcaaacac aaaaagccaa gcagttatcc
      961 gcacctttac gcggtgctgt cagtgctgca aactctgcta tgcttaattg gggcgatgag
     1021 ttatatggcg gcttaggggc tttaggtgcg gctgttacag gtaatgatat tggtcaagca
     1081 tatcaagaaa accgtgattt aattcgcggc attgatacaa gttttcgtga ggagtaccca
     1141 aacacggcac ttggcacaca aatagccgcc tcactgccag cgactgttta caatccttta
     1201 cccttaaaga catcaaactt agcattaaca ggact
//
LOCUS       DAOQMB010000722         1233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_99185_length_1233_cov_12.125637, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000722 DAOQMB010000000
VERSION     DAOQMB010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99185_length_1233_cov_12.125637"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1096
                     /locus_tag="PLY05_13485"
     CDS             86..1096
                     /locus_tag="PLY05_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HNC04350.1"
                     /translation="MKLHALALSVVLVLVASQVQAAPKVYGQLNVSVESYQKDNQNPA
                     TDDEDYTRLQSNASRFGVKGEDELTAKLSAVYGIEWQINTDGEGNDLGQRNRYVGIKH
                     ADFGTVKAGRFDTYLKTSQGKTDLFNDFLGDIEFTIAGEDRANNAIAYESPIFKNVQF
                     NLLSQTQDVATKATNGSSASIVYDNQELGLYAALGINQSIVGRTALFGSTREGDGYRL
                     AVSYNVADLTVNALYSTNEKYDGNDGEKAYLLGAAYKINDIVLKAQYSSAEADDANKI
                     ATGNSIEKNVFSVGADYNLTTKTRAFVWYTNREDVKKDAVIVVPNDIEETSLALGVEH
                     KF"
BASE COUNT          378 a          212 c          270 g          373 t
ORIGIN      
        1 aactaacttt tttgacccct cttaaaaagt taacttatca cttaagtccc tttttcacat
       61 ttatcttata tttttggagt tcatcatgaa gcttcacgct ttagctttgt ctgttgtttt
      121 agtattagtg gctagtcaag tacaagcagc ccctaaagtt tatggtcaat tgaatgtttc
      181 ggtggaaagt tatcaaaaag ataatcaaaa ccctgccaca gatgatgaag attatacccg
      241 tttacaaagt aatgcctcac gttttggtgt taaaggtgaa gatgaattaa ccgctaaatt
      301 aagtgcggtg tatggtattg aatggcaaat taataccgac ggagagggta atgatttagg
      361 gcaacgcaat cgttatgtgg ggattaaaca tgccgatttt ggtacggtaa aagcaggacg
      421 ttttgatacc tatctaaaaa catctcaagg caaaacggat ttatttaacg acttcttggg
      481 tgatattgaa tttaccattg caggtgaaga tcgcgccaat aatgccattg cttatgaatc
      541 accgattttt aaaaacgttc aattcaatct cttaagccag actcaagatg ttgctactaa
      601 agccactaat ggctcatcag catccattgt ttatgacaat caagaattag gtttgtatgc
      661 cgcattaggg attaatcaaa gtattgttgg tcgtacagcg ttatttggct caacacgaga
      721 aggtgatggt taccgtttag cggtgtctta taacgttgcc gatttaactg tgaatgcctt
      781 gtatagcacc aatgaaaaat atgacggtaa tgatggtgag aaagcttatt tgctgggcgc
      841 agcatacaag attaatgaca ttgttttaaa agcccaatac agcagtgccg aagcagacga
      901 tgccaataaa attgccacag gcaatagcat tgaaaaaaat gtgtttagtg ttggtgcgga
      961 ttataaccta accactaaaa cgcgtgcttt tgtgtggtac accaaccgtg aagacgtgaa
     1021 aaaagatgcg gtgattgttg taccaaatga tatcgaagaa acctctttgg cgttgggtgt
     1081 ggaacataag ttttaagcca tacatggctt gatgacgttg ttcttgagga gcataagtat
     1141 gaaaacctct cgtttaattg ttgctggttt acatggtagt gaggcagagc attggcaatc
     1201 ttggtggcag ttacaagacc ctcaagcaca acg
//
LOCUS       DAOQMB010000723         1231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_99489_length_1231_cov_19.172619, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000723 DAOQMB010000000
VERSION     DAOQMB010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99489_length_1231_cov_19.172619"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1231
                     /locus_tag="PLY05_13490"
     CDS             <1..>1231
                     /locus_tag="PLY05_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNC04351.1"
                     /translation="LYYRYESAFFQVVTAEHLSKVIMPFYKRFVSDWQSIFVDTPLAT
                     LPQYQLDHMFALFFQIRRAFHFIFRAILGTSRTAAALRMSVWQSIFGYDIRRYHRSLY
                     SRMHEVSTLILGESGTGKELVASAIGLARYIPFDGKSQRFEGDYWQDFQAVHLAALPL
                     TLLESELFGHKKGSFTGATSDRAGYLETAQHSQSVFLDEIGELPAEVQVKLLRVLQNR
                     RFQRVGDNQSREFYGKIISATHRDLGAAIEQGHFRADLYYRLCSDIIRTPSLATQLAD
                     NADELHHLITLVTAKIVNTDIANEVAKEAHDWIRQHLPNYTWAGNMRELEQCVRSILI
                     HGAYYPARGGNIKDCKQQFANEIMAGNLDHDTVIGRYYALVYAQTQNYEAAAQRLNTN
                     WRTVKSRMDMAFLERLRE"
BASE COUNT          331 a          222 c          290 g          388 t
ORIGIN      
        1 ttgtattatc ggtatgaaag tgcctttttt caggtggtga cggctgaaca tttaagcaaa
       61 gtcattatgc cgttttataa acggtttgtg agtgattggc agtcgatttt tgttgataca
      121 ccgttagcaa ccttgccgca atatcagctt gaccatatgt tcgcgttgtt ttttcaaatt
      181 agacgcgcct ttcattttat ttttagagca attttaggca catcacgcac tgctgctgca
      241 ctgcgtatgt cggtttggca atcaattttt ggttatgaca ttcgccgtta tcatcgttcg
      301 ttgtatagcc gtatgcacga agtatcgacg ctcattttgg gtgaatctgg tacaggcaaa
      361 gaattggttg cctcggcgat tggtttggcg cgttatattc cctttgatgg caaaagccaa
      421 cgttttgagg gggattattg gcaagatttt caagcggtgc atttagccgc tttaccgtta
      481 acactgttag aaagtgagtt gtttggtcac aaaaaaggct catttaccgg tgcaaccagt
      541 gaccgagcag gttatttaga aacggcacaa catagccaaa gtgtgttttt ggatgaaatt
      601 ggtgagttac ctgccgaagt ccaagtaaaa ttattaagag tgttacaaaa ccgccgtttt
      661 caacgggtcg gtgataatca aagtcgtgag ttttatggca agattatttc ggcgacgcat
      721 cgtgatttag gcgcagcgat tgaacaaggc cattttagag ccgatttata ttatcgtttg
      781 tgttcggata ttattcgtac accctcttta gccacacaac tggctgataa tgctgatgaa
      841 ttacatcatt taattacctt agtcaccgct aaaattgtca ataccgatat tgccaatgaa
      901 gtggccaaag aagcacatga ttggattaga caacacttac ccaactatac gtgggcaggg
      961 aatatgcgtg agttagagca atgtgtgcgt agtattttga ttcatggcgc gtattatcct
     1021 gctcgtggtg gcaatatcaa agattgtaaa cagcaatttg ccaatgagat aatggctggt
     1081 aacttagacc atgatacggt gattggtcgt tattatgcct tggtgtatgc acaaacgcag
     1141 aattatgaag ccgctgctca acgtttaaat accaattggc gtactgttaa aagccgtatg
     1201 gatatggcat ttttggaaag gttaagagaa t
//
LOCUS       DAOQMB010000724         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_100019_length_1228_cov_7.524297, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000724 DAOQMB010000000
VERSION     DAOQMB010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100019_length_1228_cov_7.524297"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLY05_13495"
     CDS             <1..1192
                     /locus_tag="PLY05_13495"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNC04352.1"
                     /translation="MVDRYPDLIIQANNQQEVIEAIKLAKAQNLKVGVRSGGHSWSAN
                     HIRQGGLLLDVSRLDAVTIDKTTMTATVEVGRSGHQLANMLLKQGLFFPAGHCKGVCV
                     GGYLLQGGFGWNSRVLGPACMSVMAIDYVNADGELVHASPEQNADMYWAARGAGAGFF
                     GVVVKFYLRLYAKPKIIGAALISFPIERLEEVYRWADAVGKDVANSVELMLIMSGHTP
                     LVNGKGIIVFAPIFADSWREAYQALAFMKTMPKGASLKIPFVPLSLEMMYSMVMQHYP
                     ENARYAVDNLWTHASIDQLLPHLHKIANSLPSAPSHMLWMNWSPPKQRPDMAFSMEDK
                     TYIALYGVWHQATQDHLFADWAVNHVRDMEHLATGCQLADENLGQRPARFVSDQHLQT
                     DVTQ"
BASE COUNT          332 a          209 c          302 g          385 t
ORIGIN      
        1 aatggtcgat cgttatcctg atttgattat tcaagctaat aatcagcaag aagtgattga
       61 ggcaatcaaa cttgccaaag cgcaaaattt aaaagtgggt gtacgttctg gtggacatag
      121 ttggtcagcg aatcatattc gtcaaggtgg cttattatta gatgtttcac gtttagatgc
      181 ggttactatt gataaaacaa cgatgactgc aactgtcgaa gtggggcgat caggtcatca
      241 attagcgaat atgttgctca aacaaggttt attttttcct gctgggcatt gtaaaggggt
      301 atgtgttggc ggctatttat tacaaggtgg ctttggctgg aacagtcgag tgttaggccc
      361 tgcttgtatg agtgtcatgg cgattgatta tgtcaatgcc gatggtgaat tagtacacgc
      421 tagcccagaa caaaatgccg atatgtattg ggcagcaagg ggggcaggtg ctggcttttt
      481 tggggtagtg gtgaagtttt atttgcgcct ttatgctaaa cctaaaataa taggtgcggc
      541 tttaattagt tttccgattg agcgtttaga agaagtttat cgttgggctg atgctgttgg
      601 caaagatgtg gccaatagtg ttgaattaat gttgattatg tctgggcata cccctttagt
      661 gaacggcaaa gggattattg tttttgcccc gatttttgcc gactcttggc gcgaagccta
      721 tcaagcctta gcttttatga aaacgatgcc taaaggcgca tcgttaaaaa ttccttttgt
      781 acctttatcg ttagaaatga tgtatagcat ggtcatgcaa cattatcccg aaaatgcacg
      841 ttatgcggta gataatttat ggactcatgc atcaatagat caattgttgc cacatcttca
      901 taaaattgct aatagtttgc cgtctgcacc ttcacacatg ttgtggatga attggtcgcc
      961 acccaaacaa cggccagata tggctttttc gatggaagat aaaacttata ttgctttgta
     1021 tggggtatgg catcaagcga ctcaagatca tttattcgcc gattgggcag taaatcatgt
     1081 gcgtgacatg gagcatttgg caacgggttg ccaattagcc gatgaaaatt tggggcaacg
     1141 tcctgcacgt tttgtcagtg atcaacattt acaaactgat gttacgcagt agcgatatga
     1201 atttgttaaa ctgttgtcat gaaaacag
//
LOCUS       DAOQMB010000725         1228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_100061_length_1228_cov_3.419437, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000725 DAOQMB010000000
VERSION     DAOQMB010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100061_length_1228_cov_3.419437"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="PLY05_13500"
     CDS             <1..634
                     /locus_tag="PLY05_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HNC04353.1"
                     /translation="ICRKPEDSLFLSAGTGAWNQGNIVCNNEVAGSTFSAVTNAREGK
                     LVRDQQYGTGQVITWMNARKIGTDWVPHPEKQFFYLVDQLSGKCLDIPGDDNALFDGQ
                     RTQVWDCQATASDQKWYYDYSTGMLHNKANPAFCLDNRGQTWNGGGIGIHTCVDHVNL
                     RFDMLSNNIIRTRGNGDFALDASGDWNGANVTSWQYHGGNNQRWKKVYPQ"
     gene            942..>1228
                     /locus_tag="PLY05_13505"
     CDS             942..>1228
                     /locus_tag="PLY05_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04354.1"
                     /translation="MATVLYFSMIGSESDQDVINQLNQDAKRHLAIPNSPINTSAHTD
                     QQRPLIEQQQLAKQLSLALSGDLAHPLAHHLRHLQQYCQQQSACLNNITQH"
BASE COUNT          378 a          270 c          220 g          360 t
ORIGIN      
        1 tatttgtcgt aaacctgagg acagcttatt cctcagtgca ggcacaggcg catggaatca
       61 aggcaatatc gtttgtaata acgaagtagc tggctcaacc ttctctgctg ttactaacgc
      121 tcgtgaaggt aaattagtac gtgaccaaca atatggaaca ggccaagtaa ttacatggat
      181 gaatgcgcgt aaaataggta cagattgggt acctcatccc gaaaaacaat ttttctattt
      241 agtcgatcaa ttgtctggca aatgtttaga tattcctggg gatgacaatg ctttatttga
      301 tggtcaacgt actcaagttt gggattgtca agcgactgct agcgaccaaa aatggtacta
      361 cgactattca acaggtatgt tacacaacaa agcaaaccca gccttttgct tagacaaccg
      421 tggccaaact tggaatggcg gcggcatagg tattcatact tgtgttgacc acgttaattt
      481 acgttttgat atgttaagca acaatattat tcgtacccgt ggcaatggtg attttgcgct
      541 agatgctagt ggcgattgga atggtgcaaa tgtcactagc tggcaatatc atggtggcaa
      601 caaccaacgt tggaaaaaag tttatcctca ataaactctt tatcgtgttt aaaagcaaaa
      661 gcttcggctt ctgcttttaa acagctagtc ttaaactttg gcgttatcag taaactttaa
      721 ggctttaacc gtcaataatt cagcattttt agtctctaga atgcgccgcc ttattatttg
      781 cccaactgac taatcatcca taaaaaaacg tcattgtgca gtctctgtac attatctata
      841 ttaaacacag gaatcattga ttccttttat cgccattaga gcaatacact atgctagttc
      901 gtcaaccaaa atttttaatt ttgtcactta tcggccttag catggcaacc gtattatatt
      961 tttcgatgat tggctccgaa tctgaccaag acgttatcaa ccaattaaat caagatgcaa
     1021 aacgtcattt agcaatccct aattcaccaa taaacacatc agcccatact gatcagcaac
     1081 gcccactcat cgaacaacaa caactagcca aacaactgag tttggcctta tcaggtgatc
     1141 ttgctcatcc cctcgcgcac cacctacgtc atttacaaca atattgccaa caacaaagtg
     1201 cttgccttaa taacattact caacactt
//
LOCUS       DAOQMB010000726         1227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_100186_length_1227_cov_5.860068, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000726 DAOQMB010000000
VERSION     DAOQMB010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100186_length_1227_cov_5.860068"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="PLY05_13510"
     CDS             complement(<1..967)
                     /locus_tag="PLY05_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011514499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HNC04355.1"
                     /translation="MRRVVVTGLGIISCIGNDAATVKKSLKEQLSGIRHNPTYAEMGF
                     KSQVSGRPDVDLDAAIDRKAKRFMGDAAAYAYLAMQQAIADAGLSKEQYAQNPRVGVI
                     AGSGGASTANVLQAVDIAREKGIKRVGPYMVPRTMTSTVVASLATAFEIHGVNYAISS
                     ACATSAHCIGNAMEQIQLGKQDVVFAGGGEEEHWSLSCLFDAMGAMASKYNDTPESAS
                     RPFSANRDGFVIAGGGGILVIEEYEHALKRGAKIYAELVGYGATSDGYDMVAPSGDGA
                     ARCMQLALAMADCPVDYINAHGTATPTGDCVELKAIQRVFGENIPY"
     gene            complement(1042..>1227)
                     /locus_tag="PLY05_13515"
     CDS             complement(1042..>1227)
                     /locus_tag="PLY05_13515"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04356.1"
                     /translation="VKFFGQILPTHQKVTYELQIKRVVERKLVMGIADGKVSVDGREI
                     YTASDLRCGLFTRTDNF"
BASE COUNT          366 a          307 c          236 g          318 t
ORIGIN      
        1 tatacggaat attttcacca aatacgcgtt gaatcgcttt taactcaaca caatcacctg
       61 tgggtgtagc cgtgccgtga gcattaatat aatcaacagg gcagtctgcc atggctaacg
      121 ctaattgcat acaacgtgcc gcaccatcac cacttggcgc aaccatatca taaccatctg
      181 acgttgcccc atagccaacc aattcggcat aaatttttgc accacgttta agggcgtgtt
      241 cgtattcttc aatgactaaa atgccaccac cgcccgcaat gacaaaacca tcacgattgg
      301 ccgagaaagg gcgagaggct gattcgggtg tatcattata tttgctggcc atggctccca
      361 tcgcatcaaa taaacacgat aagctccaat gttcttcttc accgccccct gcaaagacaa
      421 catcttgctt gcctaattga atttgctcca tggcattgcc aatacaatga gcagaggttg
      481 cacaagcaga agaaatagca taattcacac catgaatttc gaaagcggtg gccaacgaag
      541 caacaacggt actggtcatg gtgcgtggta ccatgtatgg gccaacacgt ttaatgcctt
      601 tttcacgagc aatatcaacg gcctgtaaga cattcgctgt tgaagctccg cctgaaccag
      661 caatcacacc aacgcgtgga ttttgtgcat attgctcttt gcttaaacct gcatcagcaa
      721 ttgcttgttg catggctaaa taagcatacg cagcggcatc acccataaaa cgtttggctt
      781 ttcggtcaat tgctgcgtct aaatcgacat caggacggcc actgacttgg cttttaaagc
      841 ccatttcggc ataagtcggg ttatgtcgaa tgccagagag ctgctctttt aaactctttt
      901 tgacagtagc ggcgtcattg ccaatacacg aaatgattcc taatcctgta acaacgacgc
      961 gacgcataaa tcaccctcta aatatatggc atcatgctaa acatcatacc gaggaaagct
     1021 taaataatta ttaaaggtaa cttaaaagtt atctgtacgc gtaaataaac cacaacgcaa
     1081 atccgaggca gtataaatct ctcggccatc aacgctaacc ttaccatcag caatacccat
     1141 gactaattta cgttcaacga cacgcttaat ttgtaattca taggttactt tttggtgtgt
     1201 aggcaaaatc tgtccaaaaa acttgac
//
LOCUS       DAOQMB010000727         1225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_100489_length_1225_cov_17.392308, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000727 DAOQMB010000000
VERSION     DAOQMB010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100489_length_1225_cov_17.392308"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="PLY05_13520"
     CDS             complement(<1..728)
                     /locus_tag="PLY05_13520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004683598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC04357.1"
                     /translation="MKSFTNKVAAITGAGSGIGRALALNLAKQGCHLALSDVNETGLA
                     ETVAQAKAFGVNVTSQKLDVSNKDGVHAWADAVVRDHGKVNLIFNNAGVALGSTVEGM
                     SYDELAWVMNINFWGVVYGTKAFLPYLKASGEGHIINISSIFGLCSQPSQSAYNASKF
                     AVRGFTEALRQELDIENAPVSVTCVHPGGIKTNIAKAAKMNDSIKSIGLNPSTSTQNF
                     EKLFRTTPAEAANVILKAVQKDSRR"
     gene            complement(895..>1225)
                     /locus_tag="PLY05_13525"
     CDS             complement(895..>1225)
                     /locus_tag="PLY05_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxyacetophenone monooxygenase"
                     /protein_id="HNC04358.1"
                     /translation="PNLFLLMGPNTGLGHSSMVFMIESQVAYVMDFLKQMNKHGWKEV
                     DVKADQQQRFNDDLQNKLGNAVWSTGCKSWYVNDSGKNTTLWPGFTFDFRRKTAQFDA
                     QNYQTSK"
BASE COUNT          338 a          262 c          239 g          386 t
ORIGIN      
        1 cgacgtgagt ctttttgtac ggcttttaaa atcacattgg ctgcttctgc aggtgttgtt
       61 ctaaacagtt tttcaaagtt ttgtgtgctt gttgacggat tcaaaccaat gctcttaatg
      121 ctgtcattca ttttggctgc tttagcaata ttggttttaa tcccacctgg gtgcacacaa
      181 gtgacgctga ctggtgcatt ttcgatatct aattcttggc gtagggcttc ggtaaagcca
      241 cgtaccgcaa atttactggc gttataagca ctttgagagg gttgagagca aaggccaaaa
      301 atactagaaa tattgataat atggccttca ccagaagctt ttaaataggg taagaacgct
      361 ttagtaccat agaccacacc ccagaaattg atgttcatga cccatgctaa ttcgtcatag
      421 ctcatgcctt caacggtgct acctaatgcc acacctgcat tattaaaaat taagtttact
      481 ttgccatgat cacgaacaac agcatctgcc caagcatgta caccatcttt attcgagaca
      541 tctaattttt gactggtcac attgacacca aaagctttgg cttgagcaac tgtttctgct
      601 aaacctgttt cattaacgtc acttaacgct aaatggcagc cttgtttggc taagttgagg
      661 gctaaagcac ggccaatacc agagccagca cctgtaatcg cagcaacttt attcgtaaaa
      721 gatttcatgg ttttctctct aaaagtaata accctttggc ttggtggtgc agcacttggg
      781 tgatgaatgg aaaatgttag ttatacgtat ggtttcgact tcgctcaacc accctgattt
      841 cacttaacca agatgtaccc tgagcggagt cgaagggtta tcttcaaaca caacctattt
      901 actggtttga tagttttgag catcaaactg ggcagtttta cgtctaaaat caaatgtaaa
      961 acctggccag agagttgtgt ttttgccact gtcgttgaca taccagcttt tgcagccagt
     1021 gctccatacc gcattaccta atttgttttg taagtcatca ttaaagcgtt gttgttgatc
     1081 ggctttcaca tcgacttctt tccagccatg cttattcatt tgttttaaga aatccatgac
     1141 ataagcgact tgtgactcaa tcataaagac cattgagcta tggcctaaac ctgtatttgg
     1201 ccccattaat aaaaataaat taggg
//
LOCUS       DAOQMB010000728         1223 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_100865_length_1223_cov_4.972603, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000728 DAOQMB010000000
VERSION     DAOQMB010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100865_length_1223_cov_4.972603"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(749..>1223)
                     /locus_tag="PLY05_13530"
     CDS             complement(749..>1223)
                     /locus_tag="PLY05_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04359.1"
                     /translation="SDINHISLTGAGAFYYGALGQIVIPIPVVGALVGSTVGYFIGNL
                     IHQSGLVALGESANVKIARERREQIEKMCLHAIPQMRAERIELERVLHSNQIARREFI
                     NDVFFNLESSLLTNDANQYITALTSLNGAFGKDLGFKSLEEFNTLMEDDEFTFKL"
BASE COUNT          366 a          223 c          247 g          387 t
ORIGIN      
        1 ggcctgtggt gatgtttccc tctttagggc aagtggtggc tttgattgtg attgtcgtct
       61 ttattgcagc aattacgttg tttggtaaag gcaaatgaac gagtcttaga tttattgagt
      121 ttactcccaa taggctaggg cagaaaaatc ctctcttctg aatagttgaa tgttatctga
      181 gtggttataa gataacatca actaatcccc ttcaatctgc ctaaacggta acgctgaatc
      241 gctaccgatt cttcgactaa aaaacgttta acggcttaag caacagcaag agcctatgtt
      301 atattgatgt tagattaccc atgaaggggg ttatttgaca ggctttttag tatttagaat
      361 tacaacatga tcgattatgg atattcaaac catcacaagg agctgttcat cggcactagg
      421 cagagggtag gtgatggatg aatttattaa aaactccctg tatttggtgg attgattgag
      481 acgactgagt tctccatgtc gttgagctgt ttacatgcga taagattgag gttgacaaac
      541 tcatcccata gcaaactatg tttataatgc ttttcgtaat agacattgat ttggcaaatt
      601 tcaggccgat ctgtataaat gttacagagc tgactgattt gattgtagtg tttacacatt
      661 ccatctccac gatctaagta agcagtcaat ggcgataagt tgacgtggcg acaacaagca
      721 ccacattgtg tgcatggaaa tggttttgtc ataatttgaa agtgaactca tcgtcttcca
      781 taagtgtatt aaactcttca agacttttaa atcctaagtc tttgccaaaa gcaccattta
      841 aacttgttag agctgtgatg tattgattag catcatttgt taagagtgaa ctttctaaat
      901 tgaaaaaaac atcattaata aactctcttc gtgcaatttg attgctgtgt aatacacgct
      961 ctagctctat gcgttcggca cgcatctgcg gaatggcatg taaacacatt ttttcaattt
     1021 gctcacgtcg ttcacgtgct attttaacat tagctgattc acctaatgcg actaagccag
     1081 attgatgaat caaattgcca ataaaatagc caaccgttga gccgactaat gctccaacaa
     1141 caggaattgg aatgacaatt tgacctaatg caccataata gaatgcacca gcacctgtta
     1201 atgagatgtg gttaatatca gag
//
LOCUS       DAOQMB010000729         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101027_length_1222_cov_11.185947, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000729 DAOQMB010000000
VERSION     DAOQMB010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101027_length_1222_cov_11.185947"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1222
                     /gene="ffh"
                     /locus_tag="PLY05_13535"
     CDS             <1..>1222
                     /gene="ffh"
                     /locus_tag="PLY05_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016142064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HNC04360.1"
                     /translation="VLQALSPGQAFVKVVFDELVKTMGDANETLNLHALPPAVVLMAG
                     LQGAGKTTTVAKLAKYLQEREKKKVLVVSCDVYRPAAIKQLETVANDVGATFFPSHKD
                     QDPVAIAQAAIAEGKLKFFDVVIVDTAGRLHVDAEMMGEIQRLHAAINPVETLFVVDA
                     MTGQDAANTAKAFNDALPLTGVVLTKMDGDARGGAALSVRMITGKPVKFMGIGEKTEA
                     LEPFHPERIAQRILGMGDVLSLVEEVERKIDKEKSEKIAKKLRKGGGFDLEDLLVQFQ
                     QMKAMGGMASFLDKLPGMSGSQMQQAAAGGAPEKMMKQMEAIIQSMTPQERRKPDIIN
                     GSRKRRIATGAGVQIQDVNRLLKQHSQMEKMMKKLATPSGITKMIRGMKGLQNKFAAG
                     GMGMPNGMQMPDVDP"
BASE COUNT          372 a          221 c          306 g          323 t
ORIGIN      
        1 aagtgttaca agctttatca ccaggtcaag cctttgttaa agtggtgttt gatgaattag
       61 tcaaaaccat gggcgatgca aatgagacat taaatttaca tgcgttgcca cccgccgtgg
      121 tgttaatggc aggtttacaa ggtgcaggta aaactacgac tgttgctaaa ttagccaaat
      181 atttacaaga acgcgaaaag aaaaaagttt tagtggtgtc ttgtgacgtt tatcgtcctg
      241 cggcgattaa acaactcgaa actgtggcga atgatgtggg tgcaaccttc ttcccatcac
      301 acaaagatca agatcctgtt gccattgctc aagccgcgat tgctgaaggt aaactcaagt
      361 tttttgatgt ggtgattgtg gataccgcag gtcgtttgca cgttgatgcc gaaatgatgg
      421 gcgaaattca acgtttgcat gcggcaatta atcctgttga gactttgttt gtggtggatg
      481 ccatgacagg tcaagacgct gccaatactg ccaaagcctt taatgacgct ttaccattaa
      541 caggtgtggt gttaaccaaa atggacggtg atgcgcgtgg tggtgcggct ttatctgtaa
      601 gaatgattac aggcaaacct gttaaattca tgggtattgg tgaaaaaacc gaagctttag
      661 aacctttcca tccagagcgt attgcccaac gtattttggg tatgggtgac gttttgtcgt
      721 tggtggaaga agttgaacgc aaaattgaca aagaaaagtc agaaaaaatt gccaaaaaat
      781 tgcgtaaagg tggcggtttt gacttagaag atttgttggt gcagtttcaa caaatgaaag
      841 cgatgggtgg tatggccagc ttcttggaca aattacctgg tatgagtggt tcacaaatgc
      901 agcaggcagc ggctggtggt gcgcccgaaa aaatgatgaa acaaatggaa gccatcattc
      961 aatcaatgac accacaagaa cgtcgcaaac ccgatattat taatggttca cgtaaacgtc
     1021 gtattgccac aggtgcaggt gtgcaaattc aagatgttaa ccgtttgttg aaacaacact
     1081 cacaaatgga aaaaatgatg aagaaattgg cgacaccatc gggtattacc aaaatgattc
     1141 gtggcatgaa aggtttacaa aacaaatttg ccgcaggcgg tatgggaatg ccaaatggta
     1201 tgcaaatgcc agatgttgac cc
//
LOCUS       DAOQMB010000730         1222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101028_length_1222_cov_9.850900, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000730 DAOQMB010000000
VERSION     DAOQMB010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101028_length_1222_cov_9.850900"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..737)
                     /locus_tag="PLY05_13540"
     CDS             complement(189..737)
                     /locus_tag="PLY05_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HNC04361.1"
                     /translation="MNKMTTRTLLMAMAMMITSVSYAQDAGYIGVQYGQFRTSDASQN
                     NGDHEDMDHIGVSIGANLLPTISLEAQYTTNVNDKQLGAGSTLSSQVIGAYAKIQTPT
                     PVYVNGRIGVARVDYELDTPLFNYTESTDGLSYGVGVGIKLADTASIEANYTRLPDLE
                     DQSFNNADIKNELLTVGVNFHF"
     gene            1031..>1222
                     /locus_tag="PLY05_13545"
     CDS             1031..>1222
                     /locus_tag="PLY05_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04362.1"
                     /translation="MRYKSTLSAALLAVFSTSAAQADTVLGVYASADYWQYEGKADVA
                     QSGKKKNNFVFDEKNQASLS"
BASE COUNT          357 a          250 c          227 g          388 t
ORIGIN      
        1 cgtgcagccg ttggggtggc ttctttgcca cgcggtgcac aagtcgaaat ggacggcgtg
       61 atggaattgg tttgatttaa ctttcagcaa aatagcccgt gtaacgtaaa acgtaacacg
      121 ggattttatg taataacaaa atattttctg tattccgcta cgctgcatac aggcttgtat
      181 ctaccctgct aaaaatgaaa attaactccc actgttaaca actcattttt gatatcggca
      241 ttattaaaac tttggtcttc caaatcaggt aaacgcgtat aattagcttc tatacttgct
      301 gtatccgcca atttaatgcc cacacccacg ccataagaca gaccatcagt actctctgtg
      361 taattaaata agggcgtatc aagctcataa tctactcgcg ccaccccaat acggccattg
      421 acataaacag gtgttggcgt ttgaattttg gcatatgcac caatcacttg gctggataat
      481 gttgagcctg caccaagctg cttgtcattg acgttagttg tatattgagc ctctaaactg
      541 atagttggca gtaaatttgc cccaatactc acgccaatat ggtccatatc ttcatggtcg
      601 ccattgtttt gactcgcatc actggttcta aattgaccat attgtacgcc tatataaccc
      661 gcatcttgcg cataactaac acttgttatc atcatggcca ttgccattaa taaagttcgt
      721 gttgtcatct tgttcatttt acacctcatg tcttagcatt gacgcgctta tcgcatcgct
      781 atattcaaat cttgttacaa atatagccgc atatattttg ataaaatgac tgttttttgt
      841 ttagaaactt caactctttg ttgtgattgg tataaaaacc gtaagaaaaa acggcattta
      901 atgaatgtca tatagcctga tgttgtattt tggtgaatga atttcagctc atttctgtgt
      961 ctgtttttga tacaagcaga aataaactgt gctataaata cgctaaattt tatataggaa
     1021 agatacaacc atgcgttata aatcaacttt aagtgcagct ttactggccg tatttagcac
     1081 ttctgcggct caagcagata ccgtattagg cgtttatgcc agtgccgatt attggcaata
     1141 tgaaggcaaa gccgatgttg cgcaaagcgg taaaaagaaa aataattttg tgtttgacga
     1201 aaaaaatcaa gcatcgctgt ca
//
LOCUS       DAOQMB010000731         1221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101214_length_1221_cov_11.782161, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000731 DAOQMB010000000
VERSION     DAOQMB010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1221
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101214_length_1221_cov_11.782161"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..592)
                     /locus_tag="PLY05_13550"
     CDS             complement(8..592)
                     /locus_tag="PLY05_13550"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HNC04363.1"
                     /translation="MPKKMIRKYLPDPEKIANIQGLGFLRHRLQDPNLWHLNRHSASG
                     AMFWGLWCALLPMPFQMVPAAIAALIFRVNLPLCIVLVWLSNPLTMIPIIYISYFVGA
                     SLLNRPMINGDTLHQIVNNLGQMVGNAFSSTPQHIKGLDLSQHLEPILLGTLVTGFLF
                     GCIGYLAMNWYWRYHVTKAWNNRQFRKATTKKEQ"
     gene            717..>1221
                     /locus_tag="PLY05_13555"
     CDS             717..>1221
                     /locus_tag="PLY05_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNC04364.1"
                     /translation="MERFLLAAIFGVLSLLIWPTLPNWQYLLIISSIIAVLSCWLKCL
                     RVVVFCTLAMTWSVWSAEQYLQRLLPHKLENKAFKVQVKIEGLSEYQSFGQRVSVKPI
                     KIVSNIDYSIDANTHWQLSAPSHLILKPQQIWQMTVTLKRPHGTASAGAFDYQAWLLS
                     ENISAIGK"
BASE COUNT          396 a          225 c          229 g          371 t
ORIGIN      
        1 tcgaagccta ttgttctttt ttggtcgttg ctttacgaaa ttggcgatta ttccatgctt
       61 tagtaacatg gtatcgccaa taccaattca ttgctaaata cccaatacag ccaaataaaa
      121 agcctgtcac taaagtacct aacaaaattg gttctaaatg ttgacttaaa tctaaacctt
      181 tgatgtgttg aggggtactt gaaaaagcat tgccaaccat ttgccctaaa ttattgacaa
      241 tttgatgcaa agtatcgcca ttaatcatcg gacgattaag cagtgaagca ccaacaaaat
      301 aagaaatata aataattgga atcatggtta acggattgct taaccacact aaaacaatac
      361 acagaggcaa atttactcta aaaattaagg cagcaattgc cgcaggcacc atttgaaaag
      421 gcatgggcaa caaagcacac cataagcccc aaaacattgc gccagaagca gaatgtcggt
      481 taagatgcca taagttaggg tcttgtaaac gatgacgtaa aaagcccagg ccttgtatgt
      541 ttgcaatttt ttcggggtca ggcaaatatt tacgaatcat ttttttaggc atagcttaac
      601 ccagttttct aaaggcgtgc gattgttaac gatttatgat gattgtgcaa ctttcttaat
      661 aatttattcg tttgaatttg taagtatcaa aaagcatttt tatggaataa aacatcatgg
      721 agcgattttt gttggcagcc atttttggcg tgctttcttt attaatttgg ccaacattgc
      781 ctaattggca atatttgctc atcatttcaa gtatcattgc tgtattaagc tgttggctca
      841 aatgcttgcg agtagtagtg ttttgcacgt tggcaatgac atggtcagtg tggagtgctg
      901 aacagtattt acagcgttta ctaccacata aactcgaaaa taaagccttt aaagtacaag
      961 taaaaattga gggattatct gagtatcagt cttttggtca gcgtgtctcc gttaagccaa
     1021 taaaaatcgt ttctaacatt gattattcca tagatgcaaa tacacattgg caactctctg
     1081 cgccttctca tctaatttta aaaccccagc aaatatggca aatgacagta accctaaaac
     1141 ggcctcatgg aacagcctca gcaggagcat ttgattatca agcatggtta ttaagtgaaa
     1201 atatcagtgc gattggcaaa g
//
LOCUS       DAOQMB010000732         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101391_length_1220_cov_18.345923, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000732 DAOQMB010000000
VERSION     DAOQMB010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101391_length_1220_cov_18.345923"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="PLY05_13560"
     CDS             complement(<1..785)
                     /locus_tag="PLY05_13560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HNC04365.1"
                     /translation="MNYQRGILANVPSVACYLTFQQYDSSQVKACLQQLCAMVDGEQI
                     VLGLGKSLLLQLDLAHPHLPNFPAYTSAGINIPSTPADVWLWVRGSERGELLLLSQQL
                     IKTLEAAFHCTQIVDAFCHLGGRDLTGYEDGTENPHDEAAIAAAFVADENPALEGGSV
                     VAVQQWLHHMPSFNKMSNKQQDNAIGRGKDDNEELDDAPESAHVKRTAQESFSPEAFV
                     LRRSMPWSSGVDGGLMFVAFGHSSQAFAAQLRRMVGLEDGIQDA"
     gene            complement(797..1168)
                     /locus_tag="PLY05_13565"
     CDS             complement(797..1168)
                     /locus_tag="PLY05_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNC04366.1"
                     /translation="MLIGELAQAAQVSRDTIRFYEKQGLITGFRLGNGYKNYTPATLD
                     TVNFIKLAQELGFSLREIASILPMLAGGQLAPVLVENFVADKVRLIDQRINSLQQLRQ
                     RLSTLPIGTDCPLRRDCTPDD"
BASE COUNT          366 a          290 c          239 g          325 t
ORIGIN      
        1 gcatcttgaa tgccatcttc aaggccaacc atacgccgta attgtgccgc aaatgcttgg
       61 ctcgaatgac caaaagcgac aaacatcaaa ccaccatcca cccctgacga ccacggcata
      121 gaacggcgca acacaaaggc ttctgggcta aaactttctt gggcagtacg tttaacatga
      181 gccgattcgg gcgcatcatc taactcttcg ttatcatctt tgcctcgacc aatggcatta
      241 tcttgttgtt tgttgctcat tttgttaaag cttggcatat ggtgcagcca ttgttgcacg
      301 gcaaccacac taccaccctc taatgcagga ttttcatcag ccacaaaagc ggcggctatt
      361 gctgcttcat cgtgaggatt ttctgtgcca tcttcgtaac ctgttaaatc acgaccaccc
      421 aaatgacaaa aagcatcgac aatttgagtg caatgaaaag cggcctctaa ggttttaatc
      481 agttgttggc ttaataacaa cagctcacca cgctcagaac cacgcaccca taaccaaaca
      541 tcggcaggag tagaaggaat attaatgcct gcactggtat aagcaggaaa gtttggcaaa
      601 tgcggatgtg ctaaatctaa ttgtaataac aaagacttgc ctaaacccaa gacaatctgt
      661 tcaccatcaa ccatagcaca taactgttgt aaacaagctt taacttggct tgaatcatat
      721 tgttgaaaag ttaaataaca ggcaacactc ggcacgtttg ctaaaatgcc acgttgataa
      781 ttcattctct attcctctag tcatcaggcg tgcagtcacg acgcaaaggg caatctgtgc
      841 caatcggcaa ggtacttaaa cgctggcgta attgttgtag gctgttaatc cgttggtcaa
      901 tgagacgtac cttatcagca acaaaatttt ctactaacac tggggctaat tggccgcctg
      961 ccaacatggg taaaatactg gcaatttcac gtaaagaaaa acctaactct tgggcaagct
     1021 taataaaatt taccgtgtct agtgtggcag gcgtgtaatt tttataacca ttacctagcc
     1081 taaagcctgt aattaagcct tgtttttcat aaaaacgaat cgtatctcgg ctcacttgag
     1141 ccgcttgagc aagttcacca attaacatgc aaaaagtcct tgaccttgga gtgtactcca
     1201 tagtttagag tgtgtatcaa
//
LOCUS       DAOQMB010000733         1220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101394_length_1220_cov_9.412017, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000733 DAOQMB010000000
VERSION     DAOQMB010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101394_length_1220_cov_9.412017"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            95..1183
                     /locus_tag="PLY05_13570"
     CDS             95..1183
                     /locus_tag="PLY05_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002134917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HNC04367.1"
                     /translation="MLNKTPPFKTNSNPAFRFIDLFAGIGGIRLPYQQQGGLCVFSSE
                     WDKFAQKTYAANFGELPAGDITQIAATDIPDHDILLGGFPCQAFSQAGLKRGFSDTRG
                     TMFFEIQRILAQKRPKAFMLENVKQLQGHEKGRTLATILDILEGRNVQELPLDIPMSD
                     DARAALSHRLNYQVFYKVLRAADFGIPQNRERIFIVGFNRDYFGESIDFTKVFNWPTP
                     PCVPTRVGDILEDSQYIDEKYTISEKLWAGHVRRKSEHLNKGNGFGYSLFNANSTHTN
                     TLSARYYKDGSEILIDQTELNKRPRKLTPRECARLQGFPENYIVDAVSDVQAYKQFGN
                     SVTVPVIRAVAHQLLTAINTAQLLRESP"
BASE COUNT          357 a          235 c          221 g          407 t
ORIGIN      
        1 tataaaagct tatttgctag ctttgagact attttaatct atgaacatat aagatacgct
       61 atttaactat agaatttata gattatttct gactgtgcta aacaaaactc cacctttcaa
      121 aacaaactcc aaccctgctt ttcgttttat tgacttattc gcaggaattg gtggtattcg
      181 tttgccatat caacaacaag gtggcttatg tgttttctcg tctgaatggg acaaatttgc
      241 tcagaaaacc tatgctgcta attttggcga gctacctgct ggcgacatta cccaaattgc
      301 tgccacagat attcctgacc atgatatttt gctagggggc ttcccttgcc aagctttttc
      361 gcaagcaggc ttaaaacgtg gctttagtga cactcggggt acaatgttct ttgaaattca
      421 acgtattctt gctcaaaaac gcccaaaagc ctttatgctc gaaaatgtta aacaattaca
      481 aggccatgaa aaaggccgta ctttagccac cattttagat attcttgaag gacgcaatgt
      541 tcaagagcta cctttagata ttccaatgtc tgatgatgca cgtgccgcat taagtcatcg
      601 tttaaattat caagtttttt ataaagtatt acgtgccgca gattttggta ttcctcaaaa
      661 tcgagaacgt atttttattg ttggttttaa tcgtgattat tttggcgaat ctatagattt
      721 taccaaggta tttaattggc caaccccccc ttgtgtgcca acacgtgttg gcgacattct
      781 agaagactct cagtatattg atgaaaaata tacaatttct gaaaaactct gggcaggtca
      841 tgttcgtcgt aaatctgagc atttgaataa aggtaatggt tttggctatt ctttatttaa
      901 tgcaaatagc acacatacca atactttaag cgcacgctat tataaagatg gtagtgaaat
      961 tctaattgat caaactgagc ttaataaacg tccacgcaaa ttaacacctc gtgaatgtgc
     1021 tcgattacaa ggttttcccg aaaactatat tgttgatgct gtttcagatg ttcaagctta
     1081 taaacaattt ggtaactctg tgactgtgcc tgtgattcgg gctgtggctc atcaattgtt
     1141 aactgctatc aatactgctc agctattaag agaatcccca tgatgtttat ttttggtagt
     1201 gctgaacaaa ttgcccaatc
//
LOCUS       DAOQMB010000734         1219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101551_length_1219_cov_12.944158, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000734 DAOQMB010000000
VERSION     DAOQMB010000734.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101551_length_1219_cov_12.944158"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="PLY05_13575"
     CDS             <1..1218
                     /locus_tag="PLY05_13575"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HNC04368.1"
                     /translation="LMEGSTYIGELRGAAKEQESIWQIIQTSRKIEKIFGKVHLTFGE
                     PIFLKDILNQENPQWQNTAFDAKAKLPWLNRTIDNLANQINTHINQAAAINPITLLSL
                     VLLSTPNHAIDEDVLIKQLNLYKHIASHLPYSSRIKITDLQGEEMLSYGVQLKAIQRV
                     KHPLGDLICVADNQAVFLTYFRNNILHLFVLPSLVACLMQHNGQLQREQISQVIVSLY
                     PYLKSELFLSWDVSEIEALVNQHIDILISSGILTIVDHVIFAPHPNTEEYAQLVILGE
                     AVKETLERYFMTISLLTERQSGSINQKQLENLCSLLAQRLSVLYEFNSPEFFDKGIFR
                     NFIDTLKKVGLLQQDEQGLLHFDSRLYEIATQANLVLREETQHTIQQITNVSDEDINS
                     AMAELERKEKKRK"
BASE COUNT          406 a          201 c          216 g          396 t
ORIGIN      
        1 ttaatggaag gtagcactta tattggtgaa ttacgtggtg ctgccaaaga gcaagaatcc
       61 atttggcaaa ttattcaaac cagccgcaag attgaaaaaa tctttggtaa agtacattta
      121 acttttggtg agccaatttt cttaaaagac attttgaatc aagaaaatcc acaatggcaa
      181 aacaccgcgt ttgatgcaaa agccaaatta ccgtggttaa atcgtacgat tgataattta
      241 gcaaatcaaa ttaacaccca tattaaccaa gccgctgcca ttaacccgat tacgctatta
      301 agtttagttt tattatcgac accaaatcat gccattgatg aagatgtatt aattaaacaa
      361 cttaatttgt ataaacacat tgccagtcat ctgccctata gctcacgtat taaaattact
      421 gatctacaag gtgaagaaat gctcagttat ggtgtacaac ttaaagccat tcaacgggtt
      481 aaacatcctt tgggcgattt aatttgtgtc gctgataatc aagcggtatt tttaacttac
      541 ttccgcaata acattttgca tttgtttgtg ttgccctctt tagtggcctg tttaatgcaa
      601 cataatggtc aattgcaacg tgagcaaatt agccaagtga ttgtttcttt atatccttat
      661 ttaaaatcag agctgttttt aagttgggat gtgagtgaaa tcgaagcttt agttaatcaa
      721 catattgata ttttgattag ctcaggtatt ttgacgattg ttgatcatgt gatttttgca
      781 cctcatccaa atactgaaga atatgctcaa ttagtcattt tgggtgaagc cgttaaagaa
      841 acacttgaac gttactttat gaccatttct ttgttaactg agcgtcagtc gggcagtatt
      901 aaccaaaaac aattggaaaa tttatgttca ttattagcac aacgtttgtc ggtgttatat
      961 gaatttaatt cgccagagtt ttttgataaa ggcattttta gaaactttat tgatacgctt
     1021 aaaaaagttg gcttattaca acaagatgaa caaggtttgt tgcattttga cagccgttta
     1081 tatgaaattg ccactcaagc caatttagta ttacgtgaag aaacacaaca taccattcag
     1141 caaattacca atgtcagtga tgaagacatt aacagtgcaa tggctgaatt agagcgtaaa
     1201 gagaaaaagc gtaaataaa
//
LOCUS       DAOQMB010000735         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101867_length_1217_cov_8.693632, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000735 DAOQMB010000000
VERSION     DAOQMB010000735.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101867_length_1217_cov_8.693632"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLY05_13580"
     CDS             <1..292
                     /locus_tag="PLY05_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002115017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC04369.1"
                     /translation="YALFLPSGLSQVSWQVIALQAAFQGVLVVIIAMLTFIAAVQRLG
                     AFTVGSFLALAPMLAATLAVPVLDEPLTLGLILGLVGMVTGALQPWRLLKKS"
     gene            complement(337..1068)
                     /locus_tag="PLY05_13585"
     CDS             complement(337..1068)
                     /locus_tag="PLY05_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04370.1"
                     /translation="MSSRLRLFCAASLSTVLMLSATPYVYAANDDLDDLQTKLGNQWV
                     LVKNDQRRNIKTWAKQEDGKRFRSFKVEAILDSNVEAGVRVLLDAESYTKWYWEVFES
                     KMLKVISPTEYYIYLKHRAPVSLPDRDVILHAIVTPQKKAGDPILLNVKAAPDYIPEA
                     PPLIRMPAEDMIAEFTPLPNNQVMVKAEGYVDPGGRVPTWANNYIQRNAPYSILLGLL
                     RMMTSDTYKNSKTPLPFKVYNHPDS"
BASE COUNT          334 a          231 c          244 g          408 t
ORIGIN      
        1 ttacgcgctg tttttgccca gtggtttaag tcaagtctct tggcaagtca ttgccctaca
       61 agccgctttt caaggtgtgt tagtagtgat tattgccatg ctaaccttta ttgccgctgt
      121 acaacgttta ggcgcattta ctgtaggcag tttcttggca cttgcaccca tgcttgcagc
      181 aacattagct gtgcctgtgt tggatgaacc attaacttta ggtttaatac tcggtttggt
      241 tggtatggtc acaggtgcgt tgcagccgtg gcgattgcta aaaaagtctt aataaaaaaa
      301 ctccccaaat ttggggagtt tttatttctg agatgtttaa ctatcagggt gattgtaaac
      361 cttaaagggt aatggtgttt tactgttttt ataggtatct gaggtcatca tccgcaataa
      421 acccaataaa atactataag gtgcattacg ttgaatgtag ttgtttgccc atgttggtac
      481 acgtccacca gggtcaacat agccttctgc ttttaccatt acctgattat ttggcaaggg
      541 ggtaaattca gcaatcatat cttcggcagg cattctaatt aatggtggtg cttcaggaat
      601 ataatcgggt gcagctttaa catttaagag aataggatca cctgcttttt tttgtggcgt
      661 aacaatggca tgtaaaatta catcacgatc aggcaaacta acaggtgcgc gatgtttgag
      721 ataaatataa tattctgttg gtgaaatgac tttcagcatt ttactttcaa acacttccca
      781 ataccatttt gtgtagcttt ctgcgtccaa taagactcta actcctgctt caacattact
      841 atcaaggata gcttcaactt taaaagaacg aaaacgtttg ccatcttctt gtttggccca
      901 cgttttgatg ttgcggcgtt ggtcattttt gaccaatacc cattgattgc ccagtttggt
      961 ttgtaaatca tctaaatcat cattcgccgc ataaacataa ggagtggcac tgagcattaa
     1021 tacagtgctt aggctggcgg cacaaaaaag gcgtaaacga gaactcataa ttcaccttgt
     1081 tatttcagac tttatataag caaagtcttt tatcttagaa ttattcttat catttggtgt
     1141 cataaacaca caaacttcat gctagcagaa cattgtttta ttctagataa aactgtgtca
     1201 ttttgtgagc agttaga
//
LOCUS       DAOQMB010000736         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101872_length_1217_cov_6.343373, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000736 DAOQMB010000000
VERSION     DAOQMB010000736.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101872_length_1217_cov_6.343373"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /gene="nagZ"
                     /locus_tag="PLY05_13590"
     CDS             complement(<1..961)
                     /gene="nagZ"
                     /locus_tag="PLY05_13590"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020798500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HNC04371.1"
                     /translation="MNGALMLDIAGYELDNEDKEILRHPHVGGLIFFARNFSGTEQLQ
                     ALVKSIRAIRPEIIIAVDQEGGRVQRFRDGFVKLPPMQAIGDLFSKDNQKACRFAYQC
                     GWLMAAEVLAIGVDISFAPVLDVDFGLSRVIGDRSIHSQPEVATALLRSYIQGMHEAG
                     MAATGKHFPGHGSVEADSHVAIPVDNRTFAEIDAFDLVPFKALSNQLQGIMPAHVIYP
                     EVDDRPAGFSPIWLQDLLRQKMGFDGVIFSDDLSMEGAGVAGGYADRADAALEAGCDM
                     VLACNNRAGAVQILEYLEKHPRMPHQHRFNALRGKGKLAWNDLP"
BASE COUNT          378 a          293 c          199 g          347 t
ORIGIN      
        1 gtggcaaatc attccatgct aattttccct taccccgtaa agcattaaaa cgatgttgat
       61 gtggcatacg tgggtgtttt tctaaatact ctaaaatttg caccgcacct gcacgattat
      121 tacaggccaa aaccatatca caacccgctt ctaaagcagc atctgctcga tcagcatacc
      181 cccctgcaac accagcccct tccatactta aatcatcaga gaaaatcacg ccatcaaagc
      241 ccattttttg acgcaacaaa tcctgtaacc aaattggcga aaatccagca ggacgatcat
      301 caacttcagg gtaaatcaca tgggcaggca tgatgccttg tagttgatta ctcaatgctt
      361 taaacggcac aagatcaaat gcatcaattt cggcaaatgt acgattatct acgggaatcg
      421 ccacatgaga atctgcctca accgaaccat gacctgggaa atgttttcct gtagcagcca
      481 tacctgcttc atgcatacct tgaatatacg aacgtaataa ggcagtagct acttctggtt
      541 gactgtgtat agaacggtca ccaatcaccc gacttaaacc aaaatctaca tctaatacgg
      601 gcgcaaaact aatatcaaca ccaattgcta agacttctgc tgccattaac caaccacatt
      661 gataagcaaa acgacaggct ttttgattgt ctttactaaa caaatcacca attgcttgca
      721 taggaggcag tttaacaaaa ccatctctaa accgttgtac acgtccacct tcttggtcaa
      781 cggcaataat aatttcgggg cgaatggctc ggatactttt taccaaagct tgaagctgct
      841 ctgtacccga aaaattgcgt gcaaaaaaga ttaatccccc cacatgagga tgacgtaaaa
      901 tctctttatc ttcgttatct aattcataac ctgcgatgtc tagcatcaat gcaccgttca
      961 tttttggctc ctatttaaga cataataaaa ttatggctaa ttctaattaa attttctctg
     1021 tatattctgt gttttttcac aatttaaccg ctacaaaatg tcgtcctaag tctctataat
     1081 ctaaaaataa gtcagtagtc cgctgacact ttatgaatat caagaatcac atggccgcaa
     1141 ttactcgtaa acatatttta tggtcagccg cttttggtgg cttagtcttt ttttcaggtg
     1201 tgtttgccaa acaagcc
//
LOCUS       DAOQMB010000737         1217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_101924_length_1217_cov_3.246127, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000737 DAOQMB010000000
VERSION     DAOQMB010000737.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101924_length_1217_cov_3.246127"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1217)
                     /locus_tag="PLY05_13595"
     CDS             complement(<1..>1217)
                     /locus_tag="PLY05_13595"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNC04372.1"
                     /translation="NNGTRDIFIKDLETGEVTRLSVDAKDGEANNDSYAPVFSPDGTK
                     IAFTSLATNLIVNDTNDVGDIFIKDLLTGEVTRISTNSKGEELNASSESLVFSPDGKK
                     VAFISSANNAVDNDTNFNVDIFVKDLETGELIRVSTTSAGQEANASSYAPVFSPDGSQ
                     ILFQSYAYNLVNNDTNDVGDIFIKDLTTGNVTLVSQSTTGVQANAFSQQASFSADGSQ
                     VVYASLANNLVENDKNSFEDVVVATLGVGGGTDTVQSSVSYTLANNVENLILLGKDSL
                     TGAGNELDNIITGTEAGDFIYGYAGNDTLSGALGDDNIEGGVGDDLVFGDDGNDYLVG
                     GEGNDTLVGGNGNDNLSGGAGNNSYIYTNGHDFINNEEYPSADEDNGIGDLLTIANFA
                     RSQAVFSQDIQIA"
BASE COUNT          372 a          290 c          174 g          381 t
ORIGIN      
        1 ttgcaatttg aatatcttgg ctaaatactg cctgactacg cgcaaagttt gcaatcgtta
       61 ataagtcacc aatgccatta tcttcgtcag cacttggata ttcttcatta ttgataaagt
      121 catggccatt tgtgtaaata taactattat tacctgctcc accacttaaa ttatcattgc
      181 cattaccgcc aacaagcgtg tcgtttcctt caccacccac taaataatca ttgccatcat
      241 caccaaagac aagatcatct ccaaccccac cctctatatt gtcgtcaccc aatgctcccg
      301 acagcgtatc attacctgca tatccataaa taaaatcacc tgcttctgtg cctgtaataa
      361 tattatccaa ctcattacca gcacctgtca gactatcttt tcctaataaa attaaattct
      421 caacattgtt tgctaaggtg taagaaactg agctttgaac agtatctgtt cctccaccca
      481 cacctaatgt agccactaca acatcttcaa aactgttttt atcattttct accaaattat
      541 tagccaagct tgcatagaca acttgagagc catctgcgga aaaagacgct tgttggctaa
      601 acgcattggc ttgtacacca gtcgtagact ggctcactaa tgttacattt cctgttgtta
      661 aatccttaat aaaaatatca ccaacatcat tagtgtcgtt gtttactaag ttataggcat
      721 agctttggaa tagtatttgg ctgccatcag gtgaaaaaac aggcgcataa ctggatgcgt
      781 ttgcttcttg gccagcactt gtggtgctaa cccgaatcaa ctctccagtt tccaaatcct
      841 taacaaaaat atcaacatta aagttagtat cattatctac ggcattattc gcagaactga
      901 taaaagcgac tttttttcca tcaggtgaaa ataccaaact ttcgctagat gcatttaatt
      961 cttcaccttt gctattggta ctaattcgag tgacttcgcc tgtgagcaaa tctttaataa
     1021 aaatatcacc aacatcgtta gtatcgttaa caattaaatt agtagccaaa ctcgtaaagg
     1081 cgattttagt cccatcaggc gaaaagacag gtgcataact atcattattc gcttcaccat
     1141 ccttggcatc cacactcaat cttgtaactt cacccgtctc taagtcttta ataaaaatat
     1201 cacgtgtacc gttattg
//
LOCUS       DAOQMB010000738         1215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_102218_length_1215_cov_30.023276, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000738 DAOQMB010000000
VERSION     DAOQMB010000738.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102218_length_1215_cov_30.023276"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..1115)
                     /locus_tag="PLY05_13600"
     CDS             complement(15..1115)
                     /locus_tag="PLY05_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HNC04373.1"
                     /translation="MNLVSIFENLPDFRVDRNKRHRLVDIMVIAVTAFICGADGWTEV
                     EDFGESKQDWFAGFLELPNGIPSHDTFCRVFRLLNPVHFEDCFLRWIESIQDKTEGEI
                     IAIDGKTLRRSYNTGKGKPAVHMVSAWANTNGLVLGQVKTEEKSNEITAIPELLSKLS
                     LAGCIVTIDAMGCQKAIATQIHDQEADYVLALKGNQGLLHDDVKLYLDHAISHKKPLD
                     YHEEVDTGHGRIETRRCWVTEDIAWLAPKAVWTGLRSICALECERIVGEKISCERRYF
                     ISSLEADATILANAVRAHWSIENNLHWVLDVVFKEDYCRAREEYAAQNMAVLRHMALN
                     LLKQENSRKRSIKNKRLKAGWDETFLLSILGF"
BASE COUNT          357 a          289 c          199 g          370 t
ORIGIN      
        1 tagggcaaac gcacttaaaa tcctaagata gagagcaaga aggtttcatc ccatcctgct
       61 ttgagtcgtt tgtttttaat actgcgttta cgtgagtttt cctgcttgag taaattaagt
      121 gccatatgac gtagtacagc catattttga gcggcgtact cttcacgcgc acgacaataa
      181 tcttccttaa atacgacatc tagaacccaa tgaagattgt tttcaatcga ccaatgcgct
      241 ctcaccgcat tcgctaaaat agtcgcatct gcttctaaac tagagataaa ataccgacgc
      301 tcacaactta tcttctcacc cacgatacgc tcacactcta acgcacaaat tgagcgcaaa
      361 cctgtccata ctgctttggg ggctaaccac gctatatctt ctgtcaccca acaacgacgt
      421 gtttcaatac gaccatgccc tgtatccact tcttcatgat aatcaagtgg ttttttatgg
      481 ctaattgcat gatctaaata aagttttaca tcatcatgca ataaaccttg attgcctttt
      541 aacgctaaaa cataatccgc ttcttggtca tgtatctgcg tggcaatcgc cttttgacaa
      601 cccatcgcat ctatagtcac aatacagcct gctaaggata atttggagag tagctcagga
      661 atggcggtta tctcattcga tttttcttct gtcttcactt ggcctaaaac caaaccgttg
      721 gtatttgccc atgcactcac catatgaacg gctggcttac ctttgcctgt gttataagag
      781 cgacgtagtg ttttgccatc aatagcaata atttcgcctt ccgttttatc ttgaatgctt
      841 tctatccaac gtaaaaaaca gtcttcaaaa tgcacaggat ttaataaacg aaaaactcga
      901 caaaaggtgt catgtgacgg aatcccattg ggtaactcta aaaaaccagc aaaccaatct
      961 tgcttggatt caccaaaatc ctcaacctct gtccatccat ccgctccaca aataaaagca
     1021 gtcaccgcaa tgaccatgat gtcgactaaa cggtgacgtt tgtttcggtc aactctgaaa
     1081 tcaggtaaat tttcaaatat tgatactaaa ttcatgtttt atatagaaat aggtgaataa
     1141 tccctctagc ctaatctcta ttttaatgaa tttaaatacc ccttagtcgc tttttattta
     1201 agtgcgtttg cccta
//
LOCUS       DAOQMB010000739         1212 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_102750_length_1212_cov_15.519447, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000739 DAOQMB010000000
VERSION     DAOQMB010000739.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102750_length_1212_cov_15.519447"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1212)
                     /locus_tag="PLY05_13605"
     CDS             complement(<1..>1212)
                     /locus_tag="PLY05_13605"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNC04374.1"
                     /translation="KKMENIEDAYYAEHLDFSGTKAFDARANYRSKSFLTVPLCNHQN
                     EVIGVLQLVNAKDTTTAETISFSQDLEPIVAALASSAAIALENQILLQDHKNLLDAFI
                     QVIAQAIDAKSSHTSAHCQRVPALTELIAQAACDAQDGALKDFNLDESDWYELRVASW
                     LHDCGKLATPDYLLDKATKLHLLNDGIETVKARFVALMSQKETQYIKQMLIEPSQQET
                     LQQQLNQLIQTLKEECAFIEVANKGGEFMSAEAKQRIKDIGQRSYVDHQGQLQRLLTD
                     KEIEFLCIERGTITFDERQKINDHMKVTIQMLESLPFPKNLKRVPEYAGGHHEKMDGT
                     GFPKGLKREQMSWPARMMAIADIFEALTASERPYKPAMKISQALSIMQRMRDQNHIDP
                     DLYQLFLQARVW"
BASE COUNT          348 a          250 c          223 g          391 t
ORIGIN      
        1 ccaaacacgt gcttgcaaaa agagttgata caagtcaggg tcaatatgat tctgatcgcg
       61 cattctttgc ataatgctta aggcttggga aattttcatt gctggcttat atggccgctc
      121 actggcagtt aacgcctcaa aaatatcagc aatcgccatc atgcgcgctg gccatgacat
      181 ttgctctcgt tttaagcctt tcgggaaacc cgtaccatcc attttttcat gatgtccacc
      241 tgcatattct ggtacacgct ttaagttttt ggggaaaggc aaagactcca acatttgaat
      301 agtgactttc atatggtcat taattttttg acgctcatca aaagtaatag tgccacgctc
      361 aatacacaaa aactctattt ctttgtcagt taatagacgt tgtaactggc cttgatgatc
      421 aacataactt cgttggccaa tatctttgat gcgttgtttc gcctcagcac tcataaactc
      481 gccaccttta ttggcaacct caataaaagc acactcctct tttagcgttt gaataagttg
      541 gttgagctgt tgttgtaaag tttcttgttg ggagggttca atcagcattt gtttgatgta
      601 ttgtgtttct ttttgtgaca ttaacgccac aaaacgtgct ttgaccgttt ctataccatc
      661 atttaataaa tgtaatttgg ttgctttatc taataaataa tctggtgttg ccaacttacc
      721 acaatcatgc aaccatgaag caacacgtaa ttcgtaccaa tcgctttcgt ccaaattaaa
      781 atcttttaat gctccgtctt gagcgtcaca ggcggcttgg gcaattaact cggttaaagc
      841 aggtactcgt tggcagtgag cagacgtatg ggaagacttc gcatcaattg cttgagcaat
      901 cacttgaata aaagcatcta ataaattttt atgatcttgc aataaaattt ggttttctaa
      961 agcaatcgct gcgcttgacg ctaatgcagc aacaataggt tctaaatcct gcgaaaaaga
     1021 aatggtttca gccgttgttg tatctttagc attaaccaac tgtaatacac caatcacttc
     1081 attttgatga ttacacaaag gaacggttaa aaatgatttg gaacgataat tagcacgcgc
     1141 atcaaaggcc ttagtccctg agaaatcaag atgctcagca tagtaagcat cttcaatatt
     1201 ttccattttt tt
//
LOCUS       DAOQMB010000740         1211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_103035_length_1211_cov_2.150519, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000740 DAOQMB010000000
VERSION     DAOQMB010000740.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103035_length_1211_cov_2.150519"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..184)
                     /locus_tag="PLY05_13610"
                     /pseudo
     CDS             complement(1..184)
                     /locus_tag="PLY05_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824646.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
     gene            complement(594..>1211)
                     /locus_tag="PLY05_13615"
     CDS             complement(594..>1211)
                     /locus_tag="PLY05_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HNC04375.1"
                     /translation="AIYSQNLITQERQVISHLKGLNGAPAWSPDGTKLALTLSQDGNP
                     EIYLFDVASKKLTRLTNHYAIDTEARWYPDGRALIFTSDRGGSPQIYRLDIASQEVKR
                     LTFEGSFNGRGAISPDGKYLAMVHRRAGQNFQIAIQDLQTGGLTRLTETPLDEAPSFS
                     PNSQMIVYATRQGNQGVLGIMSLDGRFKMQLPASEGQVREPAWSP"
BASE COUNT          313 a          255 c          256 g          387 t
ORIGIN      
        1 ctaaagccat gttgtattca cgagtaccac gttcgtcggc atgaccagaa agcaatactt
       61 tggcttgagg attttgattt aagtaactgg catgtgcttg caaggtttct aaatcatcgc
      121 tgcttaaatc gctgctgtca tagtcaaaat gaactaagcg tgtgcttaac agagcagctt
      181 ggctgctact attacttcca ccattactgc tatcatcttg acctgtacca tcatcagcat
      241 tttggccatt aacacctgta cctgtacctt tgtttttgct agcatttgct aaggcttctg
      301 cttttaaacg ctcttgttca gcaagggcag cagcttgcgc ttctgcagct tgtttgtctt
      361 ggtcagcttg actttgggtt tgggtttgat cggttacgtc cgtcgtgttt gattttccct
      421 ttaaggtgct acagccagcc atagcgattg ttaaactaat agcgattgct aaaggaagtt
      481 tagtgcttaa catcagtctt tctccagaag agggcttata taaccaataa gtcctcatga
      541 tttaaacctt gtactatttg acgataatgt aaatagtccc taagtaaata aaattaaggc
      601 gaccatgcag gttcgcgtac ttgtccttct gaggcaggta gttgcatttt aaaacggcca
      661 tctaaagaca taatacctaa cacgccttga ttaccttgtc gagttgcata aacaatcatt
      721 tggctgttgg gcgagaagct tggtgcttca tccagtggcg tttccgttaa tcgggttaaa
      781 ccaccagttt gtaaatcttg aatggcaatt tgaaaatttt ggcctgcacg gcggtgtacc
      841 atagcgagat atttaccgtc agggctaatt gcgccacggc cattaaatga accttcaaac
      901 gttaaacgtt tgacttcttg gctggcaatg tctaaacgat aaatttgtgg gctaccgcct
      961 ctgtctgagg taaagattaa tgcacgacca tcaggatacc aacgcgcttc ggtatcaatt
     1021 gcgtaatgat ttgtaagcct tgttagtttt ttgctagcca catcaaataa ataaatctct
     1081 gggttaccgt cttgtgacaa tgttaatgct aactttgtgc catcaggcga ccatgcaggt
     1141 gcaccattta agccttttaa atgactaatg acttggcgct cttgagtaat taaattctgg
     1201 ctataaatgg c
//
LOCUS       DAOQMB010000741         1208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_103429_length_1208_cov_10.408500, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000741 DAOQMB010000000
VERSION     DAOQMB010000741.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1208
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103429_length_1208_cov_10.408500"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(176..595)
                     /locus_tag="PLY05_13620"
     CDS             complement(176..595)
                     /locus_tag="PLY05_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04376.1"
                     /translation="MPYSFGELVVMTRPVKLTERTPGHAMVAISIAGVGEEAWGFYPK
                     GVKDEIAEGGWHRYNRQSVIPISKKQYTDMKTEIEKWKSKDYIMGYRDCTDFALAVLK
                     AANISVPKDSIATLPDDFGADIVSLNGGGGGRCLARP"
     gene            707..1078
                     /locus_tag="PLY05_13625"
     CDS             707..1078
                     /locus_tag="PLY05_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04377.1"
                     /translation="MSEEKTQIADGVASELNAELGFEDKRYVVVGNFAELDSSIRLGS
                     KVYVVGGRNGDGFQNRKCVVRFRNGNLRSVWVKMKKLKNFRAAWLPQHIKEIAADDSE
                     VMTKDEAVRVSILLNEQHTQT"
BASE COUNT          395 a          256 c          237 g          320 t
ORIGIN      
        1 gcgtaaggcg caagcaccct acaacaaaat aaacaaaaac tcagtaatca ctgaagtcga
       61 aaatagcccc ataattggct gggtgcttgt cgcacttgac gcgaaagtta gacttggggc
      121 tggcacgaca aaaaatcgtc atttaaataa caatactatt tactccctaa agtctttatg
      181 gccgagcaag acagcgccca cctcccccac cattaagaga tactatgtca gcaccaaaat
      241 catcagggag agtagcaata ctatctttag gaacagaaat attagctgct ttcaacactg
      301 ccaaagcaaa atccgtacaa tctctatatc ccattatata atctttagac ttccattttt
      361 ctatttcagt tttcatatca gtatattgct ttttagaaat aggtatgact gattgacgat
      421 tgtatctatg ccatcctcct tctgctattt catctttgac accttttggg taaaaccccc
      481 acgcctcctc accgacacca gcaattgaaa ttgccaccat agcatggcca ggtgttcttt
      541 cagtcaattt gactggcctt gtcataacaa ctaactcacc aaaagaatat ggcatataca
      601 tcccctccta accaaaaacc acgtctatat aaaaataaaa cacgaagtct aactactgca
      661 ttaacaggcg caaacccaaa caaattacga ttaacgagag gttactatga gcgaagaaaa
      721 aactcaaata gcggacgggg ttgcgtccga gttaaatgct gagttaggtt ttgaagataa
      781 aagatacgtt gtcgttggaa atttcgcgga attagatagc agtataaggc ttggctcaaa
      841 agtctatgtt gttggaggta gaaacggcga tggattccag aatcgtaaat gtgtagttag
      901 atttagaaat ggaaacctac gcagcgtatg ggtaaagatg aaaaagctca aaaattttcg
      961 tgcagcatgg ttgccacagc acataaaaga aatagcagcc gatgactcgg aggttatgac
     1021 taaggatgag gcagttcgtg tttccatatt attaaacgaa cagcacacgc aaacctaact
     1081 actagcttaa cgtgcgccac cccacacgtt ataagcctgt gcaagtgaat cacttttatt
     1141 tttaatgctt agacagtggc tggggtggtg tcactgttaa agcgtgagtt aggggtattt
     1201 atggatat
//
LOCUS       DAOQMB010000742         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_103734_length_1206_cov_11.410078, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000742 DAOQMB010000000
VERSION     DAOQMB010000742.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103734_length_1206_cov_11.410078"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLY05_13630"
     CDS             complement(<1..634)
                     /locus_tag="PLY05_13630"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HNC04378.1"
                     /translation="MKPLNTRALAAQVISAVIAQQLSLSQSLPPAQQRCTERDRALLQ
                     NLVYGTVREWLYFQAMIDVLVKKPIASPVVQVLLSLGMYQLLRTRIPAHAAIGETVEA
                     CKQLGFTAQAGLVNAVLRRFSQEHESICAQVIHEQHAHPQWLIKQLRKDWPKAVEQIL
                     TANNQPAALTLRVNQRKISREAYLALLAEQQIAAEVCQFSPVGVRLSELHD"
     gene            complement(631..>1206)
                     /locus_tag="PLY05_13635"
     CDS             complement(631..>1206)
                     /locus_tag="PLY05_13635"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF014908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNC04379.1"
                     /translation="GDKETGITIMQMDEGLDTGAMLYKMACPISEHDTSASLHDRLAL
                     LGGDALLTVLEAIELDEPFTPEAQDNELATYAAKLSKEEGLINWSLPAIQISRAIRAF
                     NPWPVCYTSAQGQTIRIWQGHRVNEQQTTAPAGMIIKADKQGLWIATGDGGVIAIDTL
                     QLPNGKALAFNDVLNSRKELFQVGTSLGNPA"
BASE COUNT          311 a          266 c          281 g          348 t
ORIGIN      
        1 catcgtgtaa ttcgctaaga cgtacaccaa caggcgaaaa ctgacaaact tcggctgcaa
       61 tctgttgttc tgctaataac gctaaatacg cttcacggct gattttgcgc tgattcacgc
      121 gtaaagtcag ggcagcaggc tgattgttgg ctgtcagaat ttgttcaacg gcttttggcc
      181 agtctttgcg gagttgttta attaaccatt gagggtgagc gtgttgctcg tgtatgactt
      241 gagcgcaaat gctttcgtgt tcttgactaa aacggcgtaa tactgcatta acaaggccag
      301 cttgtgctgt aaagccaagc tgtttacagg cttctaccgt ttcgccaata gcagcatggg
      361 caggaatacg agttcttaat aattgataca tgcctaaact taatagcact tgtaccacag
      421 gtgaggcgat tggttttttg actaaaacat caatcattgc ctgaaaatac agccattcgc
      481 gcaccgtgcc atagactaag ttttgcaaca atgcgcggtc acgttctgtg cagcgttgtt
      541 gagcaggcgg caaggattga ctaagtgata gttgttgagc aatcaccgcg ctaatcactt
      601 gtgcagccaa cgcccgtgta tttaacggct tcatgctggg ttgcctaagc tagtccctac
      661 ttggaataac tctttgcgtg aattaagcac atcattaaac gctaaggctt tgccattagg
      721 cagttgtaag gtgtcaatgg caatcacgcc accatcacct gtggcaatcc acaaaccttg
      781 tttgtcggct ttgataatca tgccagcagg ggctgtggtt tgttgctcat tgacacgatg
      841 accttgccaa atacgaatcg tttgaccttg ggcactggtg taacagactg gccaaggatt
      901 aaacgcacga atggcgcggc taatttgtat ggcaggtaaa gaccaattaa ttaaaccttc
      961 ttctttactg agttttgcgg cataagtcgc gagttcatta tcttgagctt caggcgtaaa
     1021 tggttcatct aactcaatgg cttctagcac cgttaataag gcgtctccac ctaatagggc
     1081 taaacggtca tgtaaactgg cggaggtgtc atgttcagaa ataggacaag ccattttata
     1141 aagcattgca cctgtgtcta aaccttcatc catttgcata atggtgatgc ctgtttcttt
     1201 gtcacc
//
LOCUS       DAOQMB010000743         1206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_103735_length_1206_cov_11.288445, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000743 DAOQMB010000000
VERSION     DAOQMB010000743.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103735_length_1206_cov_11.288445"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..642
                     /locus_tag="PLY05_13640"
     CDS             184..642
                     /locus_tag="PLY05_13640"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HNC04380.1"
                     /translation="MKRFIYPYNPKLKELARQLRNNATLAEVLLWQQLKNKQMLGYDF
                     HRQKPILNYIVDFFCHELALAIEIDGISHDSELAIIKDQQRQTDIESLNINFLRFSDE
                     DVKKNLEGVLIMINNWIIAQVNQPTPNPSQEGNFIAITLNHHANIEKISP"
     gene            639..>1206
                     /locus_tag="PLY05_13645"
     CDS             639..>1206
                     /locus_tag="PLY05_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005406962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HNC04381.1"
                     /translation="MITKLLLANRGEIACRIIKTARRLGMRTVAVYSTADANALHVQM
                     ADEAFCIGNPPSRESYLCADKIIATALACGAQAIHPAYGFLSENAEFAAKVAEAGLLF
                     VGPSAHAILAMGGKSQAKALMQKAGVPLIEGYHGEAQDQDTLIAQALKIGLPVLLKAS
                     AGGGGKGMRSVFVAEELPEAIQAAQREGQ"
BASE COUNT          366 a          244 c          253 g          343 t
ORIGIN      
        1 tgaaggcaaa gaaggcttaa gtgcattttt agaaaaacgt cctgctggat ggttatcgca
       61 atgataatat acttattgcc gttttttcta aaacttgtag agccactcag gaatagcaaa
      121 aatgcactga taagtcccct cttgagaggg gatttagggg tgtgtttaca aggaggactt
      181 accatgaaac gttttattta cccctataat ccaaaactaa aagaattagc cagacagtta
      241 cgcaataatg cgactttggc agaggtttta ctgtggcaac aacttaaaaa taaacaaatg
      301 cttggttatg actttcatcg tcaaaaacct attttaaatt atattgttga cttcttttgt
      361 catgagcttg ccttagctat agaaatagac ggtattagcc atgactctga actagctatc
      421 attaaagacc aacaacgaca aactgacatt gagtctttaa atatcaactt cttaagattt
      481 agtgatgaag atgtgaagaa aaatttagag ggcgtgttga ttatgattaa taattggatt
      541 atcgcgcaag ttaatcaacc cacccctaac ccctcccaag aggggaactt catcgctatc
      601 acactaaatc atcatgctaa cattgagaaa ataagcccat gataactaaa ctattacttg
      661 ccaatcgtgg tgaaattgct tgtcgtatta ttaaaaccgc gcgtcgttta gggatgcgca
      721 ccgttgcagt ctactccacc gctgatgcca atgccttaca tgtgcaaatg gctgatgaag
      781 ctttttgtat tggcaatcca ccaagccgcg aatcgtattt atgtgccgat aaaatcatcg
      841 ccaccgctct tgcttgtggc gcacaagcga ttcaccctgc ttatggcttt ttatccgaaa
      901 atgctgagtt tgctgctaaa gttgctgagg caggattatt atttgttggc ccttctgccc
      961 atgctatttt ggcgatgggt ggcaaatcac aagccaaagc cttaatgcaa aaagcaggtg
     1021 taccgttaat tgaaggctac cacggtgaag cacaagatca agacacatta attgcacaag
     1081 cgctaaaaat tggtttacct gtgttgttaa aagcctctgc gggtggtggt ggcaaaggta
     1141 tgcgctcggt atttgttgcc gaagaattac ccgaagcgat tcaagccgcg caacgtgaag
     1201 gccaaa
//
LOCUS       DAOQMB010000744         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_104078_length_1204_cov_15.214970, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000744 DAOQMB010000000
VERSION     DAOQMB010000744.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104078_length_1204_cov_15.214970"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..990
                     /locus_tag="PLY05_13650"
     CDS             91..990
                     /locus_tag="PLY05_13650"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNC04382.1"
                     /translation="MSPSREQIQGCLFGLALGDALGAPYEGGILERLLWKMIGSTKEG
                     YAKWTDDTQMTLDLLESILEIGHLDEQHLANKFAHSYRWRRGYGAGTAKILKRIKAGE
                     SFQTAAKAVYPNGSYGNGAAMRSSVLALIYKDKQALIEATTKTAHITHAHPLAVEGAL
                     LISLSTHFLLKQLSPPAILVELTAYIKQPSLIQRLDVINDWLTTGYLATPKEIIRLLG
                     HGVTAESSCPTALYVAFSHLHHPFEQMLSLIIQCGGDVDTIAAMAGTLWGTANGYKQL
                     PKTIIEDYQRLLSVAEGVEKFVR"
BASE COUNT          379 a          227 c          231 g          367 t
ORIGIN      
        1 tttaatcttt aaatttaaag caatttagca acaacaacaa tcatttagat aaaattaaac
       61 gtcaggttaa gacgctaaaa ggaaatcaat atgtctcctt ctcgtgagca aattcaaggt
      121 tgtttatttg gtttggcttt aggtgatgcc ttgggtgcac cttatgaagg tggcattttg
      181 gaacgattat tatggaaaat gattggttca accaaagaag gttatgccaa atggacagat
      241 gacacccaaa tgacgttgga tttattggag tcaattttag aaattggcca tttagatgag
      301 caacacctag ccaataaatt tgcccatagt tatcgttggc gacgtggtta tggcgcaggc
      361 acagccaaga ttctcaaaag gatcaaagcg ggcgagtctt ttcaaaccgc tgcaaaagca
      421 gtctatccaa atggctcgta tggcaatggt gcagccatgc gctcttctgt attggcatta
      481 atttataaag ataaacaagc acttattgaa gcaacaacaa aaacggctca cattacccac
      541 gcccacccct tagccgttga aggcgcatta ttaatatcac tcagcacaca ttttttgctc
      601 aaacaattat cgccaccagc aatactagtt gaattaactg cctatattaa acaaccttca
      661 ttaattcagc gtcttgatgt gattaatgac tggttaacaa caggttattt agctacgcct
      721 aaagaaatta ttcgtttatt gggacatggg gtgacggctg aaagttcttg tcccacagca
      781 ctttatgtgg ctttcagtca tttgcatcac ccctttgaac aaatgttgtc attgattatt
      841 caatgtggtg gagatgttga taccattgcg gcgatggcag gcacattatg gggaacggcg
      901 aatggttata agcaattacc caaaaccatc attgaagatt atcaacgttt attaagtgtc
      961 gctgagggcg tggagaaatt tgtacggtga ctccgttttc tcactaaatt ttttgtgggt
     1021 ttcgtttctc aactcacact acaaaactat tgtactgata aacaaaaaaa cgatagatat
     1081 ttgtggacaa caatgctaca gaatactatt gcttcaatag ttgaccaaaa agccatctaa
     1141 acttatgcta aattagcttt actataaaac ttagtcataa ctagtattta cttgtcttat
     1201 ttta
//
LOCUS       DAOQMB010000745         1204 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_104137_length_1204_cov_3.073977, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000745 DAOQMB010000000
VERSION     DAOQMB010000745.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104137_length_1204_cov_3.073977"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1204)
                     /locus_tag="PLY05_13655"
     CDS             complement(<1..>1204)
                     /locus_tag="PLY05_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction endonuclease subunit R"
                     /protein_id="HNC04383.1"
                     /translation="LTIEQLEEIKLKDGIRLHEHTKVHLINYAQTTGQKLVKPFVLII
                     ARDTTHAAQLKAKIQSDDFFNGQYQDKVIQVDSSTKEEEVTQSLLTVEDANNPTEIVI
                     HVNMLKEGWDVTNLYTIIPLRAANARTLIEQSIGRGLRLPYGKRTHVDEVDRLSIVAH
                     DRFQEILDEANRSDSVIRLKSVILEAEDTNAPIVSLKSETKLNQLLGQRSPQANQPID
                     SQATINATAPTPIAPQLSQIVDVSSPSIQQVIQTVQTEIQRYAAKPQLVKHSQDLSTP
                     KIQQDMMERVQQSLKTQVVMQPTLAGLTTSEPLAEVKLAKIVEEVTRLYINNTIDIPR
                     IVVTPSDDVQTGFNAFTLNTQGLRLQPNDRELMGQNLRNHQLFSIKAQELAQEKTPDD
                     LQYDFEHTI"
BASE COUNT          319 a          231 c          244 g          410 t
ORIGIN      
        1 gaatcgtatg ttcaaaatcg tactgtaggt cgtctggcgt tttttcttgt gctaactctt
       61 gagctttaat actaaatagt tggtggttac gcaaattttg ccccatcaac tcgcggtcgt
      121 taggctgtaa acgtaaacct tgtgtattga gggtaaaagc attaaaacca gtttgtacat
      181 catctgaagg tgtgaccaca atgcgcggaa tatcaatggt attattgata tataaccttg
      241 tgacttcttc aacaatttta gccagtttta cctcggctaa aggctcagat gttgttagtc
      301 ctgctaatgt tggttgcatg acaacttgtg tttttaagga ctgttgcaca cgctccatca
      361 tatcttgttg tattttgggt gtgcttaaat cttggctgtg ttttaccaat tgcggcttag
      421 cagcataacg ctgtatttct gtttgaaccg tctgaataac ttgttgaatc gacggtgatg
      481 acacatcgac aatttgtgat aattgaggcg caattggtgt aggtgcagtc gcattaattg
      541 ttgcttgact atctataggt tgattagctt gaggactacg ctgacctaat aactgattaa
      601 gcttagtttc actctttaaa ctgacaatag gcgcatttgt gtcttcagcc tccaaaataa
      661 ccgactttaa tctaatcaca gagtctgaac gattagcttc atcaagaatt tcctgaaatc
      721 tatcatgggc aacaatactt aatctgtcca cttcgtcaac atgggttcgt ttgccataag
      781 gtaagcgtaa tccgcgccca atcgattgct caatcagagt acgagcgttg gccgcacgca
      841 agggaatgat ggtataaaga ttggtaacgt cccacccttc ttttagcata ttgacatgaa
      901 tcactatttc tgtgggatta ttcgcatctt ctaccgttaa taggctctgc gtgacttctt
      961 cttctttggt gctactatcc acctgaataa ctttgtcttg atattggcca ttaaaaaaat
     1021 catctgactg aatttttgct ttaagctgag cggcatgagt agtatcacga gcaataatta
     1081 acacaaaagg cttgaccaat ttttggcctg ttgtttgagc ataattgatt aaatgaacct
     1141 tagtgtgttc atgtaagcga ataccatctt tgagtttaat ttcttcaagt tgttcaatcg
     1201 ttaa
//
LOCUS       DAOQMB010000746         1203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_104253_length_1203_cov_13.933798, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000746 DAOQMB010000000
VERSION     DAOQMB010000746.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104253_length_1203_cov_13.933798"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="PLY05_13660"
     CDS             <1..701
                     /locus_tag="PLY05_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004746506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04384.1"
                     /translation="GADTLEGGLGDDVYYVDNIGDVVTELAGEGTDKVNSTVSYTLTA
                     NVEQLYLTGIAGLSGTGNGQNNIIYGNSGNNLLSGGAGNDSLNGGAGNDTLDGGQGND
                     TLVGGLGNDTYLLDVGSGRDTIDNKDSVGNDVLLLGTGVTAEQVWLRQLGNDLEVSII
                     GTANSAKVKGWYGTDAESKIDSVQLSDGRSLLASEVQTLVEAMAAFAPPALGQTTLTN
                     EQQAALSTVITTSW"
     gene            complement(728..1180)
                     /locus_tag="PLY05_13665"
     CDS             complement(728..1180)
                     /locus_tag="PLY05_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04385.1"
                     /translation="MKKTAFFLALSLFTTACMQMPSNSNMNGQPRPAFKDTTIGMATV
                     TGLIVAMEVKRQKTQDSLRKQTTPDGISKYQARLTAMDSVKNNLVSYSSGQLSLGDKI
                     SLINATSDIVRTKLPEYGDIISTLSTLSQVLMTAQHQAMQAAQQASGK"
BASE COUNT          342 a          202 c          290 g          369 t
ORIGIN      
        1 gcggagcaga tactttagaa ggtggcttgg gtgatgatgt ttattatgtg gataatatag
       61 gcgatgttgt cactgagtta gcaggtgaag gtacagacaa agttaatagc actgtcagtt
      121 acaccttaac cgcgaatgtg gagcagttat atttaacagg tattgcagga ttatcgggta
      181 caggtaatgg ccaaaacaat attatctatg gtaacagtgg taataaccta ttgagtggcg
      241 gtgcaggcaa tgacagctta aatggtggag caggtaatga caccttagac ggcggacaag
      301 gtaatgatac cttagtcggt ggtttgggta atgataccta tttgttggat gtgggttcag
      361 gccgagacac gattgataac aaagacagtg ttggtaacga tgtgttgtta ttgggtacag
      421 gtgtaaccgc cgaacaagtg tggttgcgtc agcttggtaa tgatttagaa gtatcaatca
      481 ttggtacagc taatagtgca aaagtcaagg ggtggtatgg tacagatgca gagagcaaaa
      541 ttgacagcgt acaattgtct gatgggcgta gtttgttagc gagtgaagta caaaccttgg
      601 ttgaagcgat ggctgctttt gcgcctcctg cattaggtca aactacatta accaatgagc
      661 aacaagctgc cttatcaacc gtaattacaa ccagttggta ataaaaaagg gcgcattgcg
      721 ccctttttta tttgccagag gcttgttgag ccgcttgcat cgcttgatgt tgggcggtca
      781 ttaacacctg actcagtgtg cttaaggtac taataatatc accatattca ggtaatttag
      841 ttctgacaat atcactggtg gcattaatca gactgatttt gtcacccaat gataattggc
      901 cacttgaata actaaccaag ttatttttaa cagaatccat tgctgttaaa cgtgcttgat
      961 atttactaat cccatctggt gttgtttgtt tgcgtaaaga atcttgagtt ttttgacgtt
     1021 ttacttccat agcaacgatt aagcctgtga cggttgccat accaatcgtt gtatctttaa
     1081 aagctggtct aggttgacca ttcatattgc tattgcttgg catttgcata catgctgttg
     1141 tgaataagct taaagcaaga aaaaaagcag tttttttcac aaaattgact cctcattttt
     1201 agc
//
LOCUS       DAOQMB010000747         1202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_104419_length_1202_cov_36.346992, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000747 DAOQMB010000000
VERSION     DAOQMB010000747.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104419_length_1202_cov_36.346992"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            505..1062
                     /locus_tag="PLY05_13670"
     CDS             505..1062
                     /locus_tag="PLY05_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNC04386.1"
                     /translation="MIVGYARTSTIEQIAGFESQLLALKKAGCEKIYQEQVSSVGNRV
                     QLDAAMEFVREHDTLVVTKLDRLARSMKDLMDIIQELDRKKVGLRILDLGIDTQTPTG
                     KLMLTILGGIAQFEREVMLERQREGVNKAKSEGKYTGRKPIDDKRKALILELDKQGLT
                     RSKIAKSVGVGQATVYRVLASNKKS"
BASE COUNT          381 a          209 c          252 g          360 t
ORIGIN      
        1 atgctcaatc actggcggag ctgatggcct tgcacaagct ctagaaacaa aaagctcatc
       61 cttagccgat gagcttttgt ctgtatcaac agactatcgc cgtaggtagc cattaataca
      121 ttacagattg taagtgctaa cgatatgagc tgtaaacttg ctcaagctgg caaacaaact
      181 tcttttgtcg cttttagggg tagttaccac gttcgtgtaa aaagtgccat ctgtgctatc
      241 aaataggcga aaatatcact atcaaaacaa ctaatctaat cataggtttt agtgaatacg
      301 tcaacatgtg ataaatcatt tttttcgcaa aaatgactaa aaatctaaaa ttaagactaa
      361 tttatccgca tttatatcgg tattgctgcc attagaccta gctttagtga gagttttcaa
      421 aaaagtatta aaagctatca ctagattata ttttataaat tgagagttat tttatcacag
      481 gctctactct aatgagaggt ttttatgata gttggttatg cacgtacctc caccattgag
      541 caaatagcag gatttgagtc tcagttactg gcattaaaaa aagcaggttg cgaaaaaatc
      601 tatcaagagc aagtgtcatc cgttggtaat cgagtacaac tcgatgcagc gatggagttt
      661 gtccgtgaac acgatacttt agttgtgaca aaacttgatc gtttggctcg ttcaatgaaa
      721 gatttaatgg atattattca agaacttgac cgaaaaaaag tgggattacg tattttagat
      781 ttagggatag atacccaaac accaacaggc aagttgatgt tgacgatact tggtggtatt
      841 gctcaatttg agcgtgaagt catgctagag aggcagcgtg aaggggttaa taaggccaaa
      901 tcagagggga aatatacagg tcgaaaaccg attgatgata agcgtaaggc gttaattctt
      961 gagctagata agcaaggact gacaagatca aagatagcta agtctgttgg tgtgggtcag
     1021 gctacagttt atcgggtgct ggcaagtaat aaaaaatcat aatctattgg ttttattaga
     1081 cttatttgat tgcgtcataa taatctgtca cagatgtcgt ttttagcagc tatgtggtaa
     1141 ttacccctaa cagggcgacc caataccttc ccttctcttt tggctctcgc caatcctgac
     1201 tg
//
LOCUS       DAOQMB010000748         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_104936_length_1199_cov_4.830420, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000748 DAOQMB010000000
VERSION     DAOQMB010000748.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104936_length_1199_cov_4.830420"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLY05_13675"
     CDS             complement(<1..271)
                     /locus_tag="PLY05_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HNC04387.1"
                     /translation="MQLSPELLYSLWLTTKLALISTALLLLICIPLAYWLNNSRSSWV
                     FFIEIVVTLPMVLPPTVIGFYLLLLFSPQYLLGQLWQSWFDMSLVF"
     gene            complement(272..1039)
                     /gene="modA"
                     /locus_tag="PLY05_13680"
     CDS             complement(272..1039)
                     /gene="modA"
                     /locus_tag="PLY05_13680"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNC04388.1"
                     /translation="MRLLLSVLWSILWLSSASAQTLYVAAASDLVYCLEDINQQFKRQ
                     YPQAQIKVTTGSSGNFLAQIKQNAPYEVYLSADSRYPKQLISENMADGQSFYVYAIGQ
                     IALWTNKKTIDVKQGWAVLDQTNITRVAIANPEHAPYGQAARNALQQAKLWTNIQSKL
                     VFGENIAQTLQFAQTGNADLAIVALSLLRSPQLAGQGQFWLIPAHLYPPLEQAMVITK
                     RGQQNPLAHQYASFIKSTSAQKIFAKYGFLPPARNLP"
BASE COUNT          394 a          246 c          217 g          342 t
ORIGIN      
        1 agaaaactaa agacatatca aaccacgatt gccataactg accaagcaaa tactgcggtg
       61 aaaataataa caacaaataa aaaccaatca ccgttggtgg caaaaccatc ggcaatgtca
      121 ctacgatctc aataaaaaac acccatgatg aacgactatt attaagccaa taagctaaag
      181 gaatacagat taataataat aacgctgtag aaattaaagc cagctttgtc gttaaccata
      241 aactatataa caactctggc gacaattgca tttatggcaa attccttgct ggtggcaaaa
      301 aaccatattt ggcaaagatt ttttgtgcac tcgttgattt aataaaactc gcatattgat
      361 gagctaatgg attttgttga cctcgtttgg tgataaccat cgcttgctct aaagggggat
      421 ataaatgcgc aggaattagc caaaactgtc cttgccccgc caattgtggc gagcgtaaca
      481 aagataaagc gacaattgct aaatcagcat ttcctgtctg agcaaactgt aaggtttgag
      541 caatattttc gccaaacact aatttagatt gaatgtttgt ccataattta gcttgttgta
      601 aggcgttacg cgcagcctga ccataaggtg catgttcggg gttggcaatc gcaactcgag
      661 taatattggt ttgatcaagt accgcccatc cttgtttaac atctatcgtt tttttatttg
      721 tccacaatgc aatctggcca attgcataca cataaaaact ttggccatct gccatatttt
      781 cactaatcaa ttgtttggga taacgactat ctgccgacaa atacacctca taaggcgcgt
      841 tttgtttaat ttgagctaaa aaattgcctg atgatcctgt tgtgacttta atctgagctt
      901 gaggatattg ccgtttaaat tgttgattaa tatcctctaa gcaataaacc aaatcggatg
      961 cagcagccac atataaggtt tgtgccgagg cagaacttag ccacaaaatt gaccaaagta
     1021 cgcttaataa gagacgcata ttcatactcg atatatttat caaaaactat aacaaacgca
     1081 tttacccagc actttatggc tttaaacctg ttgctggcgc agccaaccac ccttcaacca
     1141 ttaacacggc ggcagttgag tctaaagaag gtaatttgcc tttgcgttgt tgaccaata
//
LOCUS       DAOQMB010000749         1199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_105061_length_1199_cov_1.882867, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000749 DAOQMB010000000
VERSION     DAOQMB010000749.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105061_length_1199_cov_1.882867"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1199
                     /locus_tag="PLY05_13685"
     CDS             <1..>1199
                     /locus_tag="PLY05_13685"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC04389.1"
                     /translation="SQGNARKTSGSYYTPDSLVQELIKSALEPVIEARLAANPTHPID
                     ALLNIKVCDPACGSGHFLLAAARRLAERLAVFRAPDGAVKPQDYRHALREVVAHCIYG
                     VDLNPLAVELCKTALWLEAFESGKPLGFLDAHIRCGNALVGVHDLSLLKNGIPEKAYD
                     ALGEDNKQVCSQLKKANKQGQEVSTGDLFEQQTTDTLPVFEDLNDLPEDTPEQIANKR
                     QRWHSYQQSRAYREARLKEDLFAAAFFARKTPDTLHLVPTNEDLTRLRKGLAPRPQVH
                     EYAGELAERYRFFHWPVEFPEVVKQGGFDVMLGNPPWERIKLQEQEFFASRHVLIATA
                     QNKAARERYIKLLAQGRLAQSIHNEPTTQQPNIAELNLYEEFLQAKHEAEAASAYARH
                     SGRFPLC"
BASE COUNT          321 a          258 c          294 g          326 t
ORIGIN      
        1 gtagccaagg taatgctcgt aaaaccagtg gcagttatta tacccctgac agccttgtgc
       61 aagagttgat taaatccgcc ttagagcctg tgattgaagc ccgtttagct gctaatccaa
      121 ctcaccccat tgatgccttg ctcaacataa aagtgtgtga cccagcttgt ggtagtggtc
      181 actttttgct ggcggctgca cgacgcttag ccgaacgcct agccgtattt cgtgctcccg
      241 atggcgcagt gaaaccacag gattatcgcc atgccttgcg tgaagtggtt gcccattgca
      301 tttatggtgt ggacttaaat cccttagcgg tagagctatg taaaacagca ttatggttag
      361 aggcctttga atcgggcaag cctttaggtt ttttagacgc gcatattcgt tgtggtaatg
      421 ccttggtagg tgtgcatgat ttaagtctat taaaaaatgg cattcccgaa aaggcttacg
      481 acgcattggg tgaagataat aagcaagtgt gtagccagct taaaaaagcc aataagcaag
      541 gccaagaagt atcaacaggc gatttgtttg aacagcaaac aaccgataca ttacctgtct
      601 ttgaagactt aaacgattta cccgaagaca cccctgagca aattgctaac aagcgtcagc
      661 gttggcatag ttaccagcaa agccgtgctt atcgagaagc acgtttaaaa gaagatttat
      721 ttgctgctgc tttttttgcg cgtaaaacgc cagacacttt gcatttagtg ccaaccaacg
      781 aagatttaac ccgtttgcgt aaaggcttag caccgcgtcc gcaggtacat gagtatgcag
      841 gagagttagc ggagcgttat cggttttttc attggcctgt tgagtttccc gaagtggtca
      901 agcagggtgg ttttgatgtg atgttaggta atccgccgtg ggagcgtatt aagctacaag
      961 agcaagagtt ttttgcttca cgtcatgtgt taattgccac ggctcaaaat aaagcggctc
     1021 gtgagcgtta catcaaacta ttggcgcaag ggcgtttagc gcaaagtatt cataatgaac
     1081 caaccacaca gcagcctaat atcgctgaac taaatttgta tgaagagttt ttgcaggcta
     1141 agcatgaagc tgaagccgcc agtgcttatg cccgccattc gggacgtttc ccgttatgt
//
LOCUS       DAOQMB010000750         1197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_105244_length_1197_cov_9.894046, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000750 DAOQMB010000000
VERSION     DAOQMB010000750.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105244_length_1197_cov_9.894046"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="uvrC"
                     /locus_tag="PLY05_13690"
     CDS             complement(<1..993)
                     /gene="uvrC"
                     /locus_tag="PLY05_13690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNC04390.1"
                     /translation="MTESKLQHILANLPHLSGVYKMLGAQGEVLYVGKAKSLKNRVSS
                     YFQKNISHPKTQALVAKICDIELTITHSEVEALLLEQNLIKTLKPPFNILLKDDKSYP
                     YIVITAKEDFPQISIQRGKKNIVQGARVFGPYPSAQSVHEALLLLQRLFQVRQCDDST
                     FKHRDRPCMQYQIKRCRAPCVGLISQPDYQQDVDSTIRFLEGKNTELQQQIGQQMEQA
                     SLALNFEAAAIYRDQLLNLRQIQTQQAVYGEQGEADVFALVIEQGLATIQVLYVRGGR
                     VLGSKNYFVTSTESESSTIMMEFLSGFYWQQERDIPSEIITNHSLSEEDTHALKH"
     gene            complement(998..>1197)
                     /locus_tag="PLY05_13695"
     CDS             complement(998..>1197)
                     /locus_tag="PLY05_13695"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC04391.1"
                     /translation="LLSPRERQMAEMVVNGQTNQDIADTMSVAPTTLSTYRTRIYEKL
                     DVENDVQLTHLALRFGVFKKD"
BASE COUNT          371 a          241 c          197 g          388 t
ORIGIN      
        1 atgttttaaa gcatgagtat cttcttctga taaactatga ttagtaatga tttctgaggg
       61 aatatctcgt tcttgttgcc aataaaaacc actcaaaaac tccatcataa tggttgaaga
      121 ctcagactct gttgacgtga caaaataatt tttactaccc aatactcgac caccacgcac
      181 atataatact tgaatagttg ctaaaccctg ctcaatcacc aaggcaaata catcagcttc
      241 gccttgttca ccataaactg cttgctgggt ttgaatttgg cgtaagttaa gcaactgatc
      301 acgatagata gccgccgctt caaaatttaa ggccaaagac gcttgttcca tctgttgccc
      361 aatttgctgt tgtaactcgg tattttttcc ttctaaaaaa cgaatcgtgc tgtctacatc
      421 ctgctgataa tctggttgac taatcaatcc gacacaaggt gcacgacaac gtttaatttg
      481 atattgcata cacggtcgat cacgatgctt aaatgtactg tcatcacatt gccgcacctg
      541 aaataagcgt tgtaataaga gtaaagcttc atgtaccgat tgagcactgg gataagggcc
      601 aaaaacacgc gccccttgca caatattctt tttaccacgc tgaatactaa tctgtggaaa
      661 atcttctttg gccgtaataa caatataagg atatgactta tcgtctttga gtaaaatatt
      721 aaatggtggt tttaaggttt taattaaatt ttgttcaagt aataaggctt caacttcgct
      781 atgagtaatc gttaattcaa tatcacaaat tttagcaact aatgcttggg ttttaggatg
      841 actaatattt ttttggaaat acgagctaac acgatttttt aacgatttag ctttaccaac
      901 atataacact tctccttgtg ctcctaacat cttataaaca cctgaaaggt gtggcagatt
      961 tgccaaaata tgttgaagtt tagattcagt cattcgttta atctttttta aacacaccaa
     1021 aacgtaaggc taaatgcgtc aattgcacat cattttcaac atctaatttt tcataaatac
     1081 gcgttctata agtacttaat gttgtaggcg caacactcat agtatccgca atatcttgat
     1141 tagtttggcc atttacgacc atttctgcca tttgcctttc acgcggagat aacaaag
//
LOCUS       DAOQMB010000751         1193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_105977_length_1193_cov_12.157293, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000751 DAOQMB010000000
VERSION     DAOQMB010000751.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105977_length_1193_cov_12.157293"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..832)
                     /locus_tag="PLY05_13700"
     CDS             complement(377..832)
                     /locus_tag="PLY05_13700"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HNC04392.1"
                     /translation="MGFFDSLKQMGKDVSNKLSNEVTKFKNKDFLNAVIAGCALVAAS
                     DGNVSPEEKRKMLGFINNSDALKVFNSDEVIESFNKITSKFEFDYEIGKAEALKIVAK
                     LKNNVDAAKLMVRVCCVIGASDGNFDQHEKQTVRIICRELGLDPSEFDL"
BASE COUNT          370 a          222 c          214 g          387 t
ORIGIN      
        1 tttcaacacc gttatgccca gcaagcacca caagccttag cacgtttgca acaagtggcg
       61 attgaaggtg gcaatatttt tgctgagttg atgaacaccg tgcgttattg ctctttaggg
      121 caaattaccg atgctttgtt ttcggtcggt gggcagtata gaaggaatat gtagctttca
      181 gtcagattaa aaaaggcgca aatttgcgcc ttttttatgt ctttgatacc caacagcaaa
      241 aagtttttac agcacgtatt accatgaata taaaaacagg tgatttggtg ttgggagacc
      301 atgtgcaaca agcggtgtaa agaataaata aaaaagtcga cttttatgtc gactttttta
      361 agtttagtta acgtgattac aaatcaaatt cgcttggatc aagacccaac tcacggcaaa
      421 taattcgcac ggtttgtttt tcgtgttggt caaaattacc atcagatgcc ccaattacac
      481 aacaaactct caccattaat ttggccgcat cgacattatt ctttaattta gcaacaattt
      541 ttaaagcttc ggctttgcca atttcataat caaattcaaa tttagaggtg attttattaa
      601 aagactcaat cacttcatcg ctgttaaata cttttaaagc atctgagtta ttaataaagc
      661 ccaacatttt acgtttttct tcgggactaa cattaccgtc actggcagcc accaacgcac
      721 aacctgcaat cactgcattt aagaagtctt tatttttaaa tttagtgact tcgttagaca
      781 atttattact aacgtcttta cccatttgtt tgagtgaatc aaaaaaaccc attatctgct
      841 cctgatgtgg attattaaag agctttgtaa ataacagaac tttaatagct aatcaacagt
      901 gcttttgtca tgcacattta aaatttagct ttgagcggat gttgcatggc ttgattttgc
      961 caaaattgtt gatttaattc aggttttgta atggtttaat ataacaggac actttgatag
     1021 gtagtattta tactgcaaca agaattgttg gctgaatgga aagggaagtt gggcttggtg
     1081 tagagcttag actcacgtca ttcaattaac attgaatgac gtgaaccaag aaaatcaacc
     1141 cactttacta gccaaatttt gccccatcac acggtttgca ccgctaatta acg
//
LOCUS       DAOQMB010000752         1192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_106176_length_1192_cov_7.835532, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000752 DAOQMB010000000
VERSION     DAOQMB010000752.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106176_length_1192_cov_7.835532"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..1067)
                     /gene="trxB"
                     /locus_tag="PLY05_13705"
     CDS             complement(117..1067)
                     /gene="trxB"
                     /locus_tag="PLY05_13705"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004663455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNC04393.1"
                     /translation="MDSRHIRLLILGSGPAGYTAAVYAARANLQPVVITGMQAGGQLT
                     TTTEVDNWPGDAHGLTGPDLMVRMQAHAERFGTEVIFDHINEVDFANKPFTLKGDSGV
                     YTADAVIIATGASAQYLGLESEQAFSGRGVSACATCDGFFYKNQKVMVVGGGNTAVEE
                     ALYLSNIASHVTLVHRRDKLKSEKILQDHLFAKQKEGKISIIWNHAVDEVLGDDSGVT
                     GVRVKSTVDGSTQVVDVAGVFIAIGHKPNTDIFAGQLEMKNGYLTVKSGSEGNATATS
                     VEGVFAAGDVADHIYRQAITSAGSGCMAALDAEKYLDNLK"
BASE COUNT          377 a          275 c          205 g          335 t
ORIGIN      
        1 tgaagtttag aggagtattt cagtcacaat ttgatgctgt tatgaatggc caagtcactc
       61 taaaccctta agaaaaattt gccttgttct taaaacaaga gcaaggcaaa ttgttattac
      121 ttcaaattat ccaaatactt ctcagcatct aacgcagcca tacagcctga gcctgccgag
      181 gtaattgctt ggcgataaat atggtcagcc acatcacctg ccgcaaaaac accttcaaca
      241 gaagttgctg tggcattacc ttcgctgcca ctttttaccg tcaaatagcc atttttcatt
      301 tctaattgac ccgcaaaaat atcggtattg ggtttgtggc caatggcaat aaatacacca
      361 gctacgtcaa caacttgtgt gctaccatca accgtacttt taacacgcac acctgtcaca
      421 cccgaatcat cacctaagac ttcatcaacg gcatgattcc aaataatgct aattttgcct
      481 tctttttgtt tagcaaataa atggtcttga agaatttttt cagattttaa tttgtcacga
      541 cgatgtacta aagtcacgtg agaagcgata ttcgataaat acaaggcttc ttcaaccgcc
      601 gtattaccgc caccaaccac catgactttt tgatttttat aaaagaaacc atcacaagta
      661 gcacaagcac tcacaccacg accactaaag gcttgttctg attctaagcc caaatattga
      721 gccgatgccc ctgtggcaat aattactgca tctgccgtat aaacaccact atcacctttg
      781 agagtaaacg gtttattggc aaaatcgact tcgttaatat ggtcaaaaat aacttctgtg
      841 ccaaaacgct cggcatgagc ttgcattctt accattaaat cagggcctgt taaaccatgt
      901 gcatcacccg gccagttgtc tacttcggtg gtggtggtta attgaccacc tgcttgcatc
      961 ccagtaatca cgacaggctg taaattagca cgcgctgcat aaacggccgc tgtataacct
     1021 gcagggccag aacctaaaat taataaacga atatgacgag aatccatgat gtgtaccttc
     1081 taaaaaatta ttggctataa atatgggttt aaaatacaaa aagcaaggca acaatggtaa
     1141 ttatgcctaa aattaaagaa acgccttggc ttatagctta tgcaattatt ac
//
LOCUS       DAOQMB010000753         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_106513_length_1190_cov_11.705727, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000753 DAOQMB010000000
VERSION     DAOQMB010000753.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106513_length_1190_cov_11.705727"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1190
                     /gene="glnE"
                     /locus_tag="PLY05_13710"
     CDS             <1..>1190
                     /gene="glnE"
                     /locus_tag="PLY05_13710"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HNC04394.1"
                     /translation="LQRLISLCAASPWIAEELARYPVLLDELLNADTLFAPPQKDQLL
                     AELRQQLLRIPLDDLEEQMRVLRIFKKSHVLRVAASDIQGTLPLMKVSDYLSWIAEAV
                     LESVIALAWQQLTAKYGYPIKLDGSIATSDFIILGYGKLGGIELGYGSDLDLVFVHDV
                     DSSADTNGTKAISGGEFYARLAQKMISLLTTATAAGQLYEVDMRLRPSGNAGALVSSV
                     KAFTDYQQQQAWTWEHQALVRARVVAGDEQLATRFYQIRQQVLAHQREPAKLQQEVIE
                     MRQKMRDHLSSQAKQKNAKIFDIKHDVGGIVDIEFMVQYGVLAWASDYPELTRYPDNV
                     RILEGFAQCGKLSLEQAEALKAAYLEYRARSHRLALAQQPAQVPDTDFIATRTLVQQC
                     WQNL"
BASE COUNT          347 a          235 c          283 g          325 t
ORIGIN      
        1 ttacagcgat taatcagttt atgtgccgcg agtccgtgga ttgccgagga gttagctcgt
       61 tatcctgtgt tgttagatga attattaaat gccgatacct tgtttgcacc accgcaaaaa
      121 gaccaactat tagctgaatt acgtcagcaa ttattaagaa taccgttgga tgatcttgaa
      181 gagcaaatgc gtgtcttaag gatttttaaa aagagccatg tgttaagagt agccgcatcg
      241 gatattcaag gcacattgcc gttaatgaaa gttagtgatt atttatcgtg gattgcagaa
      301 gccgtattgg aatctgtcat cgcgttagca tggcagcagt taaccgctaa atatggttat
      361 ccgattaaat tagatggcag tattgccacg agtgatttca ttattttggg ttatggcaag
      421 ttaggcggca tcgaattagg ttatggctca gatctagatt tagtctttgt tcatgatgtt
      481 gatagtagtg ccgataccaa tggcacaaaa gcgattagtg gtggcgaatt ttatgcccgt
      541 ttagcgcaaa aaatgattag tttattaacc acagccaccg ccgcaggcca actctatgaa
      601 gttgatatgc gtttacgacc ttcgggtaat gcgggtgcat tggtcagtag tgtcaaagcg
      661 tttaccgatt atcagcaaca acaagcatgg acatgggagc atcaagcctt agtacgcgca
      721 agggttgtag caggtgatga acagttagca acacgctttt atcagattcg tcagcaagtt
      781 ttagctcatc aacgagagcc agccaagttg caacaagagg tgattgaaat gcgccaaaaa
      841 atgcgtgacc acctgtccag ccaagcaaaa caaaaaaatg ctaaaatttt tgacataaaa
      901 cacgatgtag ggggtattgt tgacatcgaa tttatggtgc aatacggagt tctagcatgg
      961 gcgagtgatt acccagaact tacccgctac ccagataatg tgcgtatctt ggaaggtttt
     1021 gctcagtgcg gcaaattaag cctcgaacag gcagaagcac ttaaagcggc ttatctagaa
     1081 taccgcgccc gcagtcatcg tttagcctta gcgcaacaac ctgcgcaagt gcccgataca
     1141 gactttattg ccacccgtac actggtacag cagtgttggc aaaatttatt
//
LOCUS       DAOQMB010000754         1190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_106553_length_1190_cov_3.229075, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000754 DAOQMB010000000
VERSION     DAOQMB010000754.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106553_length_1190_cov_3.229075"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1190)
                     /locus_tag="PLY05_13715"
     CDS             complement(<1..>1190)
                     /locus_tag="PLY05_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNC04395.1"
                     /translation="NSLIASPSHEIQTRFSPQGVAISLRSKAWLDRDFVLELKELAQH
                     SWALMANNSEQEQVVLLGLVPQVPQKQHRHIKILVDCSGSMGGDRIEAAKRSLTTFIN
                     YLQPTDAFSLSRFGSQVEHAEENLRFADRNMKNFAQQWVSRLQADMGGTEMKQALYAT
                     FNLTKQNGADVLLITDGDINDINPTVNMAKKFQQRIFVVGIGSSVAEPLLRQLAEETG
                     GACEFVNDNEPVEEAINRTLVRLGQAQLQNIRMNWPKEVEVLWQSPLPKSVFYGDTLA
                     VYARVKGLTTNHSVSLLGVDDLENQTLFAQIKAQVIDKNMMLVLERMAANSEITSLLA
                     DSYQAQALALSLKHRLVNQLTSYCLVLERAEQEKLLDLPEQVNVKQMLAAGWGGTTRS
                     EHVHV"
BASE COUNT          348 a          275 c          191 g          376 t
ORIGIN      
        1 acatgtacat gctcactcct tgttgttccg ccccaacctg cggccaacat ttgtttgaca
       61 ttcacttgtt ctggtaaatc taacaatttt tcttgctcag cacgctccaa taccaaacaa
      121 tacgaagtaa gctgattaac caaacgatgc tttaacgaca aagctaaggc ttgagcttga
      181 taagaatctg ccaataaact tgttatttct gaattagccg ccattctctc aaggactagc
      241 atcatatttt tatctataac ttgcgcttta atttgtgcaa ataaggtttg attttcaaga
      301 tcatctacac ccagcaaact gacactatga tttgtcgtta aacctttaac acgtgcataa
      361 acagctaagg tatcgccata aaacaccgat tttggtaaag gcgactgcca taaaacctcc
      421 acttcttttg gccaattcat acgaatattt tgtaattgtg cttgacctaa acgcaccaaa
      481 gtacggttta tcgcttcttc aactggttca ttatcattaa caaattcaca agctccacct
      541 gtttcttcag cgagttgacg taataaaggc tctgccacac tactgccaat ccccacgaca
      601 aaaatacgtt gctgaaactt tttagccata ttaaccgtgg gattgatgtc attaatatca
      661 ccatcggtaa ttaatagtac atccgctccg ttttgtttag tcaaattaaa agtagcatat
      721 aaggcttgtt tcatttctgt accgcccata tctgcctgta atctgctcac ccactgttga
      781 gcaaagtttt tcatattacg atctgcaaat cgtaaatttt cctctgcatg ttctacctga
      841 ctaccaaaac gtgacaagct aaacgcgtct gttggttgta aatagttaat aaacgttgtt
      901 aacgaacgtt tagctgcttc aatcctatcg cctcccatag accccgaaca atcaaccaat
      961 attttgatgt gccgatgttg tttttgtggt acttgtggta ctaaacctaa taacactact
     1021 tgctcttgct ctgaattatt cgccattaat gcccacgaat gttgggctaa ttcttttaat
     1081 tcaagcacaa aatcacgatc aagccatgct tttgagcgca aagaaatcgc taccccttgt
     1141 ggagaaaatc tagtttgaat ctcatgactg ggtgaagcaa tcagtgaatt
//
LOCUS       DAOQMB010000755         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107028_length_1187_cov_7.939046, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000755 DAOQMB010000000
VERSION     DAOQMB010000755.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107028_length_1187_cov_7.939046"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLY05_13720"
     CDS             complement(<1..445)
                     /locus_tag="PLY05_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNC04396.1"
                     /translation="MSQKQFSLATKTVTKDVLVKPNIVSNAPHFNGQRFFNPWQREER
                     NQAALLKWILSRDNRTWPENLSNQQFAKPTAHFSDNLADWKMWFVGHATVLLQMGSYN
                     LLTDPVWAERCSPFKYAGPKRVRPAGIALEDLPPIDAILLSHNHYD"
     gene            complement(426..>1187)
                     /locus_tag="PLY05_13725"
     CDS             complement(426..>1187)
                     /locus_tag="PLY05_13725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC04397.1"
                     /translation="QIYVRNQQGEVLPLASVLTMQETISPAELNHFNKLRSAKITASL
                     AEGYTVGQAVSFLQQSMKEINNPSMSYDWAGQTREFLTSGDALAKAFILAVLFIYLVL
                     AAQFESFRDPLIIMFSVPLAMLGAVLALKFTGGSLNVYSQIGLITLVGLITKHGILLV
                     EFANQLQEQGRSKLQAVIESASLRLRPILMTTAAMVLGAIPLAIAVGAGAEVRQPIGW
                     VIVGGMTIGTLFTLFVIPCLYLLIGRVHQHESKTI"
BASE COUNT          368 a          281 c          213 g          325 t
ORIGIN      
        1 ggtcataatg attatgactt aataaaatcg catcaattgg cggtaagtct tctaaggcaa
       61 tgcctgcagg tcggacacgc ttaggccctg catatttaaa cggactacaa cgctctgccc
      121 acacagggtc ggttaataag ttataactcc ccatttgtaa taacaccgtg gcatgaccga
      181 caaaccacat tttccagtca gccaagttat cgctaaagtg cgctgttggc ttggcaaact
      241 gttgattgct tagattttca ggccatgtgc gattatcacg gcttaaaatc cattttaata
      301 atgccgcttg attgcgttct tcgcgctgcc acggattaaa aaatcgctga ccgttaaaat
      361 ggggtgcatt actgacaata ttcggtttga ctaacacatc cttagtcaca gttttggtag
      421 ccaaactaaa ttgtttttga ctcatgctga tgcacccgac caatcaataa atataaacaa
      481 ggaatgacaa acaaggtaaa tagcgtacca atggtcatgc cacccacaat tacccaacca
      541 attggctgac gcacttctgc tcctgcacct acggcaatcg ctaaaggaat cgctcctaat
      601 accatcgccg cggtggtcat taaaatcggt cttaaccgta aactcgccga ttcaatcacc
      661 gcttgtaatt tactacgacc ttgctcttgg agttggttgg caaattcgac caataaaata
      721 ccatgtttag taattaaacc gacaagagta attaagccaa tttgactata aacattcaaa
      781 ctcccaccag taaatttaag agccaatacc gcacctaaca tcgccaatgg cacagaaaac
      841 ataataatca aagggtctct aaaactttca aattgagcgg ctaataccaa ataaataaat
      901 aacactgcca agataaaggc tttggccaag gcatcacccg aggttaaaaa ctcgcgtgtt
      961 tgtcctgccc agtcataact catactaggg ttgttaattt ctttcatgct ttgttgtaaa
     1021 aaactcaccg cctgcccaac ggtataacct tcggctaaac tagcagtaat tttggccgaa
     1081 cgtaatttgt taaaatgatt taactctgct ggactaatcg tttcttgcat agtcaacaca
     1141 ctggccaacg gtaatacttc accttgttga ttacgcacat aaatttg
//
LOCUS       DAOQMB010000756         1187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107033_length_1187_cov_6.093640, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000756 DAOQMB010000000
VERSION     DAOQMB010000756.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107033_length_1187_cov_6.093640"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PLY05_13730"
     CDS             <1..929
                     /locus_tag="PLY05_13730"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HNC04398.1"
                     /translation="AIGILMIIRQRQHWLRMLFIWRGSVLPRILPQLAAVTGLAIVIT
                     VIHGQVFALKVPLTFVPFTLIGVALAVFLGFRNNASYDRYWEGRKLWGNMLSESRTLT
                     RQYLSLVNESQQQQSFVYGLIALSHALRHQLRGTDPSHDLQRLLHVDVYQTLKNARFI
                     PNLLILNLAEQLQKQRLEYKLEPVLAAQMEPPMARLTESISGCERLNSTPLPYTYVVI
                     LHRTAYLYCFLLPFGLVDAIGWTTPIMVAFVAYTFFALEALSDELEEPFGTSPNDLAL
                     EAMCVMIETTLKEMLGEKELPEAVRAVDFVVL"
BASE COUNT          320 a          208 c          287 g          372 t
ORIGIN      
        1 cggctattgg aattcttatg attattcgtc agcgtcagca ttggttacgg atgttgttta
       61 tttggcgtgg ctcagtgcta ccacgaattt tgccccaact cgctgcggtg acaggcttgg
      121 cgattgtcat cacagtgatt catggtcaag tttttgcctt aaaagttccc ttaacctttg
      181 taccgtttac cttaattggc gtggctttgg cggtgttttt aggctttaga aataatgcta
      241 gttatgaccg ttattgggag gggcgcaaat tatgggggaa tatgttaagt gaaagccgaa
      301 ccttaacccg tcaatatttg agcttagtca atgagagtca gcaacaacaa agttttgtgt
      361 atggtttgat tgcattaagt catgctttgc gtcatcaatt acgtggtaca gacccaagtc
      421 atgacttaca acgattatta catgtagatg tttatcaaac gctaaaaaat gcccgcttta
      481 ttccaaattt attgatttta aaccttgccg agcagctcca aaaacaacgc ttagaatata
      541 agcttgagcc agttttagcc gcacaaatgg agccaccaat ggcaaggtta accgagagta
      601 ttagtggttg tgagcgttta aatagtacgc ctttgccgta tacctatgtg gtgattttgc
      661 accgtacggc atatttatat tgttttttat tgccgtttgg tttggttgat gcgattggtt
      721 ggacaacacc gattatggtg gcatttgtgg catatacttt ttttgcttta gaagccttaa
      781 gtgatgaatt agaagagccg tttggcacta gccccaatga tttagcttta gaggctatgt
      841 gtgtgatgat tgaaacaacg ctaaaagaaa tgttgggtga gaaagagttg ccagaagcgg
      901 tgagggcggt ggattttgtg gtgttgtgat taaagcgatt taattttgaa gagcattaac
      961 aggaaagcca ataggttgct ctggaaaatc ttgaatgggt aatgttttgg agcgaccacc
     1021 ccaataaagc tttttgctat ccatgcgaat gcctataaag aatgggtcta taaaaatgta
     1081 ttctaaataa aataggcata aaaaagcccc gacttattca gtcagggctt ttttggaatc
     1141 ttggtgccta gagccggact cgaaccggca cgcccgcaaa ggcggtg
//
LOCUS       DAOQMB010000757         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107208_length_1186_cov_6.040672, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000757 DAOQMB010000000
VERSION     DAOQMB010000757.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107208_length_1186_cov_6.040672"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLY05_13735"
     CDS             complement(<1..337)
                     /locus_tag="PLY05_13735"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNC04399.1"
                     /translation="MDKNKSFRFSKDKLDNLPLPKTDRDTYTDTVETALRLRVGKTGI
                     KAFILFRKVNGKPILVTLGRYPAMTIDQARTEARKQLGLMVTGVDPNKAKKAERAKGT
                     TLNQCLEDYL"
     gene            939..1160
                     /locus_tag="PLY05_13740"
     CDS             939..1160
                     /locus_tag="PLY05_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HNC04400.1"
                     /translation="MSKKALSKYIILALLVVCGGGLAWRNKIIEHQASERFKWGMVYK
                     EGSHGYPRDAAKAVEWFHKAAGFVAQITV"
BASE COUNT          349 a          223 c          231 g          383 t
ORIGIN      
        1 ataaataatc ttctaaacat tggtttaatg ttgtgccttt ggctcgttct gcttttttgg
       61 ctttgtttgg gtcaacacct gtcaccatca aacctaattg ttttcgggct tcggttcggg
      121 cttggtcaat ggtcatcgct ggataacggc caagtgtgac caaaatcggt ttaccattca
      181 ctttgcgaaa tagtataaaa gcttttatgc ctgttttacc tactctcagt cttagtgcag
      241 tttctactgt atcggtatag gtatctctat ccgttttagg caaaggcaaa ttatctaatt
      301 tatctttgct aaacctaaag cttttgtttt tatccataat tacacctaaa tcacaaattt
      361 actggattac tgctggatta cccatggctt accgcttaca gtggttttca atatatcttg
      421 agtgataagc cgtgatgtta ataccttgat aataaagggc ttgagtgact tacagtgtag
      481 cacagttatt tacagtgatg tatttcccta aaactcataa tgctggggtc acaggttaga
      541 gccccgtcat agccaccaat caaaagtata tatatcaaag ggttgcataa tagcagccct
      601 tttttgtttc tgaattccta gtcactgttg aagttgttat cggcaacgca gttcacacac
      661 acaacattaa aaccccctaa tattgaaagt ggcaaagtac ttctataaga tgcaaaatca
      721 gggataaacg actagaaagg aaaatgctag aagatggatt aatagcaaca ttgccgatcc
      781 aagcgaagat aagaaaaagt atattaaaga aaattgctaa attcttttaa tgcaaaattt
      841 tccctatttc tcgtagatac tattatttgt cgtagttctc acagctatca cacgtgatta
      901 agttatttaa tctggttctg atttacaatg aggccgttat gtcaaaaaaa gcattaagca
      961 agtacatcat ccttgccttg ttggtggtct gtggcggagg attggcttgg cgtaacaaaa
     1021 ttattgaaca ccaagcctct gagcgattca aatggggcat ggtatataaa gaaggctcac
     1081 atggttatcc tcgggatgct gccaaagcag tagaatggtt tcataaagct gctggctttg
     1141 ttgcacaaat tacggtttag gcataagttg cccttacccg ataatg
//
LOCUS       DAOQMB010000758         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107209_length_1186_cov_5.859416, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000758 DAOQMB010000000
VERSION     DAOQMB010000758.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107209_length_1186_cov_5.859416"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..992)
                     /gene="cas6"
                     /locus_tag="PLY05_13745"
     CDS             complement(105..992)
                     /gene="cas6"
                     /locus_tag="PLY05_13745"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HNC04401.1"
                     /translation="MLPIIQLKIEITQQANLQLPEYAGSMLRGAFGHTFKRLACITKQ
                     KECKTCPLYQSCAYSQVFEGVMSDDEQHSLPYVIQMPQEGNRFIAKGQTWSFGMVLMG
                     NAINQLPIIAYAWQKACEDGFSKDQSKAQLLAIYQNDQIIYQPNKSLLPHQPHQMASK
                     PIATNRVHLDFVSPLRIQHQNYIVLKPEELTAPIILIALAKRIQRLAELHANPVELDL
                     AQLLAAAQQINIEPHLKRATMTRYSNRQQRLMNLDGLIGRISLTGDLSAFNELLILGQ
                     QVHLGKNATMGLGQYHLSL"
BASE COUNT          346 a          223 c          234 g          383 t
ORIGIN      
        1 aacaaattca tatcgctact gcgtaacatc agtaacgtaa tcatgtaatg actaaatttt
       61 atttgagagg aggtgaggga taggtaaatt gcaagcttgt gtaattatag acttaaatga
      121 tactgcccca aacccattgt ggcatttttg cctaaatgca cttgttgacc aagtattaat
      181 aactcgttaa atgcactcaa atcacctgtt aagctaattc gtccaataag accatctaag
      241 ttcattaatc gttgttgacg gttagaataa cgggtcatgg tggcacgttt gagatgtggc
      301 tcaatgttaa tttgttgtgc agccgctaat aactgtgcaa ggtctaattc cacaggattt
      361 gcatgtaatt cggcgagtcg ttgaatacgt ttggctagcg caattaaaat aatgggtgca
      421 gttaactctt cgggctttaa tacaatatag ttttgatgct gtatgcgaag cggactcaca
      481 aagtctaagt gaactctatt agtcgctatg ggtttagatg ccatttgatg cggctgatgg
      541 ggcagtaaag atttattggg ctgataaatg atttgatcat tttgataaat agccaataac
      601 tgtgctttgc tttgatcttt gctaaaaccg tcttcacacg ccttttgcca agcataggca
      661 ataatcggta attgattaat agcattaccc attaacacca tgccaaaaga ccacgtttga
      721 cctttggcaa taaaacgatt tccttcttgt ggcatttgaa tgacataggg caaagagtgc
      781 tgctcgtcat cactcatcac cccttcaaac acttggctat aagcacacga ttgataaagc
      841 ggacaggttt tgcattcctt ttgtttggtt atacaggcta accttttaaa ggtgtgacca
      901 aatgcaccac gcagcattga acctgcatat tcgggtagtt gtaaatttgc ctgttgtgtt
      961 atctcaattt ttaactgaat aattggcagc atatagttct cagtcaactt aatgtaaggc
     1021 tttatcaact ttttctaaat aaaaattatg accgtcttta cgcataaaga tacgatgatg
     1081 gtcatctaaa tcacattccc acaagctttt atgttggctt ccttgaagtt gttttggatc
     1141 caaagacttt agagctaact tacagtcact ttccgcgtag acagct
//
LOCUS       DAOQMB010000759         1185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107389_length_1185_cov_12.604425, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000759 DAOQMB010000000
VERSION     DAOQMB010000759.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107389_length_1185_cov_12.604425"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..544
                     /locus_tag="PLY05_13750"
     CDS             170..544
                     /locus_tag="PLY05_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019520249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HNC04402.1"
                     /translation="MLTIYGIKNCDTMKKAFTWLDGQGIAYQFHDYKKAALDEKTLDD
                     WLNRVGWQAIVNQKGTTWRKLALDKNTLDNQSIKPIILQNLSMIKRPLLVLDNNDIVL
                     GFDVEQWQHFAPSTMLRAGQAQ"
     gene            762..>1185
                     /locus_tag="PLY05_13755"
     CDS             762..>1185
                     /locus_tag="PLY05_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04403.1"
                     /translation="MAAFNRDHLIIGALGFVLGSVVTTASITAVLMTQSKEEKVCPPV
                     TAIVAPSASVEAAPATTEKTATTPPPVAMPTPAPITPTVAPTPKTTTTPPPPPVNTAK
                     IKGLAGEEAERVSEDQKQLVERKKNLESQLKDSNEIVRL"
BASE COUNT          379 a          256 c          218 g          332 t
ORIGIN      
        1 tgacgctcaa acccgtagta aagtgggctt aaatgaattg ttgcgtgttg attaattaac
       61 atagacgtta tgattatctg tagggataac ccttgtttat tccattaagg gcaaccacaa
      121 gggttgcccc tacaaaattc gtccttttta atttttcgaa tgattcatta tgctcaccat
      181 ttatggcatt aaaaactgtg acaccatgaa aaaagctttt acatggttag acggtcaagg
      241 cattgcttat caatttcatg attataaaaa agcagcttta gatgaaaaga ccttagacga
      301 ttggttaaat cgtgtgggtt ggcaagcgat tgtcaatcaa aaaggcacaa catggcgcaa
      361 gttggctttg gataaaaata ccttagataa tcaaagtatt aagccgatta ttttgcaaaa
      421 cttaagcatg attaaacggc ctttgttggt gttggataat aacgacattg tgttgggttt
      481 tgatgtggaa caatggcaac atttcgcccc ttcgactatg ctcagggctg gccaagctca
      541 atgaccattt tttgtgatta aatttattcc taatttagcc ttcatatatg cagcattgca
      601 tcatttttaa cgataaaata taaaaattcg gtgcattatg ctaccgcgaa tgacacccta
      661 ctacattaag tttaacaaac gtcatccaca gttgacagac accccatact tcgccactat
      721 ttcatttttt tagtctctat aataaataag gaacaataaa cgtggcggca tttaatcgcg
      781 atcatttaat tattggtgca ttgggttttg tattaggtag tgttgtcacc acagccagca
      841 ttaccgccgt attaatgact caatcaaaag aagaaaaagt gtgtccacct gtcactgcca
      901 ttgttgcccc tagtgcgagc gtcgaagctg cccctgcaac aacagagaaa acagctacca
      961 cacctccccc cgtcgccatg cctacacctg ccccaataac gcctacggtt gctcccaccc
     1021 ctaaaacaac tacaacccca ccaccgccac ctgtaaatac cgctaaaatt aaaggccttg
     1081 caggtgaaga agctgaaaga gtatcagaag accaaaaaca gttggttgaa cgcaaaaaaa
     1141 atcttgaatc acaactcaaa gacagcaacg aaattgtgag actta
//
LOCUS       DAOQMB010000760         1183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_107732_length_1183_cov_17.500000, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000760 DAOQMB010000000
VERSION     DAOQMB010000760.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107732_length_1183_cov_17.500000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1121
                     /locus_tag="PLY05_13760"
     CDS             159..1121
                     /locus_tag="PLY05_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HNC04404.1"
                     /translation="MKQYTQLSQEERYDIQAALKSKTSVTELSRTLNRSRSTIYRERS
                     RNQGKRGYRAKQAHELSKARHYKRISAVTEFGKGYIDCLLRQGWSPEQISGALKHRGW
                     DDVPSHEWIYQYVYTNKAAGGDLHAYLRHQKTYRKRGLKGNDRRGQLVNRTSIHERSQ
                     EIEERQRLGDFEADTIIGKHHKGAILTLVERKSLYTHIVHLGSTRASKKTIGCCIERL
                     QLSHAYSVTFDNGKEFAEHQQLQTRGIDTYFADPYRSNQRARNENTNGLIRQYLPKSS
                     AFDTVSDTQIQAIEEALNNRPRKSLGWFTPSQVMANFYTVALAA"
BASE COUNT          382 a          247 c          246 g          308 t
ORIGIN      
        1 ttagtattac cgactgtaag tatcataaaa cacctttttt acaccttttt taataggcgg
       61 attcaataac gaagtgcgac acatttgtta gtggttgaaa aagttaggtg tattagaata
      121 caagctgatt ttttcaactc gcaattggaa agcacaacat gaagcaatac acccaacttt
      181 cacaagaaga aagatacgat attcaggctg ccttgaaaag taaaacatca gtcactgaat
      241 tatcaagaac gctgaatcgc tcacgctcaa ctatttatcg agaacgcagt cgcaatcaag
      301 gtaagcgagg ctaccgtgct aaacaagcgc atgagttatc aaaagcacgc cactataagc
      361 gtattagcgc agtcactgag tttggtaagg gttatattga ctgcctactt cgtcaaggtt
      421 ggtcgcccga acaaatttct ggcgcattaa agcatcgtgg ctgggacgat gttccctcgc
      481 atgaatggat ataccaatac gtttacacaa acaaggcagc aggtggagat ttacatgctt
      541 atctcagaca tcaaaaaacg tatagaaaac gtggacttaa aggtaatgac agaagaggac
      601 agttagtcaa tcgcacgtct attcatgagc gttctcaaga gattgaagag cgacaacgat
      661 tgggtgactt tgaagcagac accatcattg gcaagcacca taaaggcgcg atactgaccc
      721 ttgttgagcg taaaagtctt tacacgcata tcgttcattt ggggtcaaca agagcctcta
      781 aaaagacgat tggttgctgt atagaacgct tacaacttag ccacgcttat agcgtgactt
      841 ttgataatgg taaagagttt gcagagcatc agcagttgca aacgcgtggc atagacacct
      901 attttgcaga cccctatcgc tctaatcaac gtgcgagaaa cgaaaatacg aatggtttaa
      961 ttcgtcagta tttgcctaaa tcgtctgctt ttgatactgt ctcggacact caaattcaag
     1021 ccattgaaga ggctcttaat aaccgaccta gaaaatcact aggctggttt actccaagtc
     1081 aggtcatggc taatttttat actgtcgcac ttgctgcttg aatccgccaa tttaaaaaat
     1141 ggtcgccgaa tcaccatagc ataactaaac agcagtaagc taa
//
LOCUS       DAOQMB010000761         1181 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108088_length_1181_cov_12.633215, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000761 DAOQMB010000000
VERSION     DAOQMB010000761.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108088_length_1181_cov_12.633215"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..164
                     /locus_tag="PLY05_13765"
     CDS             <1..164
                     /locus_tag="PLY05_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HNC04405.1"
                     /translation="KSLFDGSLQGIHRTDKPAFSFQGHPEASPGPHDADKLFDHFIEL
                     MTTYVSQRN"
     gene            296..1024
                     /locus_tag="PLY05_13770"
     CDS             296..1024
                     /locus_tag="PLY05_13770"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003626.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNC04406.1"
                     /translation="MTALSVNINKIALIRNSRGRNYPDVNYFAERFLQLGASGITLHP
                     RPDQRHARYQDVVELKALCDRYQQPLNVEGYPTPDFLAVVKQNRPAQCTLVPDDPQQV
                     TSDHGWDLRQHAEFLQQICQELTPLGIEVSLFVDYDNADIALAKEIGAARVELYTEPY
                     AEAFGTADEEAVWQQFARAAQLAQHAGLGVNAGHDLNLQNLPRFVQIPNILEVSIGHA
                     LIVECLEQGIEATMQAYRQILQTR"
BASE COUNT          329 a          253 c          270 g          329 t
ORIGIN      
        1 acaaatcctt gtttgatggt tctttacaag gtattcatcg taccgataaa cctgcattta
       61 gcttccaagg ccatcctgaa gcaagtccag gcccacacga tgctgataag ttgtttgacc
      121 attttattga gttaatgacg acttacgtat cgcagcgtaa ttagtgtgtt ttgttaaagt
      181 aaatttatca ggcgaagtaa ttattgccct cactctaact ctctcccaca gggagagaga
      241 actccctctc ctttgggaga gggttggggt gagggttaac aagagtaaac aaggtatgac
      301 cgctttatct gtcaacatca ataaaattgc gctaatccgc aattcgagag ggcgtaatta
      361 ccctgatgtt aactactttg ctgagcgttt tttgcagctt ggtgcaagtg gcattacctt
      421 gcatccacgt cccgaccaac gtcatgcgcg ttatcaagat gtggttgaac tcaaagcctt
      481 atgtgatcgt tatcaacagc cattaaatgt tgagggttat ccaacccctg actttttggc
      541 ggttgtgaaa caaaatcgtc ctgcacaatg taccttagtc cctgatgacc cacagcaagt
      601 tacctcagat catggttggg atttgcgcca acatgctgag tttttgcagc aaatttgcca
      661 agagctaacg cctttgggta ttgaagtcag cttgtttgtt gattatgaca atgctgatat
      721 tgccttagcc aaagaaattg gtgcagcacg ggttgagttg tataccgaac cgtatgctga
      781 agcgtttggc acagctgatg aagaggctgt atggcaacaa tttgcgcgtg ccgcacaatt
      841 ggcacaacac gcaggtttag gggtcaatgc aggacatgat ttgaatttac aaaacctacc
      901 aaggtttgtc caaattccaa atattttaga agtttccatt ggtcatgcat taattgtgga
      961 atgcctagaa caaggtattg aagcaacaat gcaggcttat agacaaattt tgcagactag
     1021 ataaacggat ttgatgcccc tcaccccaac cctctcccac agggagagag aactccctct
     1081 ccctgtggga gagggtcggg gtgagggtaa ctaaaaccaa atccacaagc gttttattgg
     1141 agtagagtga tgccaaaacg gcaagacctt aaaagcattc t
//
LOCUS       DAOQMB010000762         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108238_length_1180_cov_11.916444, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000762 DAOQMB010000000
VERSION     DAOQMB010000762.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108238_length_1180_cov_11.916444"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            162..>1180
                     /locus_tag="PLY05_13775"
     CDS             162..>1180
                     /locus_tag="PLY05_13775"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNC04407.1"
                     /translation="MSQSFIKADATYTAWLKGIKQSFAQTQLKAAVSVNTALLEFYWQ
                     LGAEIVDKQKSSQWGDGFLAQLSQDLSAEFPDVKGFSKRNLWAIRQWYLFYAQDSAIV
                     QQAVAQLAQIPWGHNLVIVSKCQSTSEALYYVQNTQQQGWSRNVLVHQIESGLYQREG
                     KAISNFEQTLPPLQSDLAQQTLKDPYVFDFLTLTKNFNERELEQGLIQHITQFLLELG
                     AGFAYVGKQVHLAVGEKDFYLDLLFYHLKLRCYVVIELKAGEFIPEYAGKLNFYLTAV
                     DKQLKTEHDNPSIGLLLCKEKDRLVAEYALTDVNKPIGISEYQLTQALPDNLKSSLPS
                     IEEIEA"
BASE COUNT          353 a          203 c          277 g          347 t
ORIGIN      
        1 aaagcagata ttgccgcaca aaaatacgac ctcagcataa accgctataa agaagtggtt
       61 tatgccgagc aaagttatgc cccacccaaa gaaattttaa aacagcttaa aagcctagag
      121 gaagagattc tgcaagacct tttagagttg gagaatatgc tttgagccag tcttttatta
      181 aagcagatgc tacttataca gcttggttaa aaggcattaa gcaatctttt gcacaaactc
      241 aattaaaagc ggcggtgagt gtaaacaccg ctttattaga gttttactgg caattgggtg
      301 cggagattgt tgataaacaa aaaagcagtc aatggggtga tggttttctt gctcagctta
      361 gccaagattt aagcgcagag tttcccgatg ttaaagggtt ttctaagcgt aatttgtggg
      421 cgattcgcca atggtatttg ttttatgctc aggattcggc aattgtgcaa caggctgttg
      481 cacaattagc gcaaattcct tgggggcata atttggtgat tgtcagtaag tgtcaaagca
      541 cctcagaggc tttgtattat gtgcaaaaca cccaacagca gggttggagc cgtaatgtgt
      601 tggtgcatca aatagaaagt ggcctttatc agcgtgaagg taaggcgatt agtaattttg
      661 aacaaacctt gccaccgtta cagtccgacc ttgctcagca aacactcaaa gacccttatg
      721 tgtttgattt tttaaccttg accaaaaact tcaatgagcg tgagttagag cagggactca
      781 ttcaacatat tactcaattt ttgttggagc ttggtgcagg ctttgcttat gtgggtaaac
      841 aagtgcattt ggcggtgggt gaaaaagact tttatttaga tttgttgttt taccatctta
      901 aattgcgctg ttatgtggtg attgagttaa aagcaggcga gtttatacct gagtatgcag
      961 gcaagctgaa tttttattta acggcggttg ataagcaatt aaaaaccgaa cacgataacc
     1021 caagtattgg tttgttgttg tgcaaagaaa aagataggtt ggtggccgaa tacgcgctga
     1081 ccgatgttaa taaacctata ggcatttctg agtaccaatt aacgcaagcc ttgcccgata
     1141 atttaaagtc tagtttgccg agtattgagg aaattgaggc
//
LOCUS       DAOQMB010000763         1180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108239_length_1180_cov_10.562667, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000763 DAOQMB010000000
VERSION     DAOQMB010000763.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108239_length_1180_cov_10.562667"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLY05_13780"
     CDS             <1..155
                     /locus_tag="PLY05_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC04408.1"
                     /translation="GHPNRYKIKLRSIGYRLVYEVIDEQVVVLVIAVGRRENDAVYQA
                     ADLRHS"
     gene            256..>1180
                     /locus_tag="PLY05_13785"
     CDS             256..>1180
                     /locus_tag="PLY05_13785"
                     /inference="COORDINATES: protein motif:HMM:NF020928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HNC04409.1"
                     /translation="MNLFSQKIAQELAVKAEQVEAAIQLLDGGATVPFIARYRKEVTQ
                     GLDDTQLRFLEERLLYLRELEDRRQTIINSINEQGKLTPELNAKLLAAETKAILEDLY
                     LPYKPKKTTKGQMAIAAGLLPLAEQLLANPSLNPQTTAESFICEGYADFKAVLEGAKY
                     ILMERFAEDAELLTKIRPYFYQQAHVISKLVDGKEQEAAKFKDYFDFSEALSTIPSHR
                     ALAVFRGRNEGFLTVTVNLPELESNVPHPCEGVIANQFDIRQQQRAADNWLQEVVRWT
                     WRIKLQPHLESSFVTELKNQADGEAIHVFGQN"
BASE COUNT          356 a          217 c          265 g          342 t
ORIGIN      
        1 cgggacatcc gaatcgttat aaaatcaaat tgcgaagtat tggttatcgt ttggtctatg
       61 aagtgattga tgagcaagtg gtggtcttag tgattgctgt tggtaggcga gaaaatgatg
      121 cggtatatca ggcagcagat ttgaggcatt cttaactcag ctaatggtgc aattaaatta
      181 aggcaactga cactgtttta gtcatcattg ctcgttataa tgccgctcag tttacttcat
      241 ggatatatgt taataatgaa tctttttagc caaaaaattg cccaagagct ggctgttaaa
      301 gccgaacaag ttgaagctgc tattcaatta ttagatggtg gggcaaccgt tccgtttatt
      361 gctcgttatc gtaaagaagt cactcaaggt ttagacgata cccaattacg ttttttagaa
      421 gagcgtttgc tgtatttgcg tgagttggaa gatcgtcgcc aaaccattat taatagcatc
      481 aacgaacaag gcaaattaac gcccgaactc aatgcaaaat tgttggcagc ggaaaccaaa
      541 gccattttgg aagatttata tttaccctat aaaccgaaaa agaccaccaa aggtcaaatg
      601 gcgattgccg caggattgtt gcctttggct gagcaattat tggcaaatcc cagtctaaat
      661 ccacaaacga ctgccgaaag ctttatttgt gaaggctatg ccgactttaa agcggtatta
      721 gaaggggcaa aatatatttt aatggagcgt tttgccgaag atgccgagct attaacgaaa
      781 attcgcccct atttttatca acaagcgcat gttattagca agttagtgga tggcaaagaa
      841 caagaagctg ctaaatttaa agactatttt gattttagcg aagcactcag caccattcct
      901 tcgcatcgtg ctttagcggt atttcgtggt cgtaatgaag gttttttaac cgtcaccgtc
      961 aatttaccag agcttgagag caatgtgcct catccctgtg agggcgtgat tgccaatcaa
     1021 tttgatattc gccaacaaca acgtgctgcc gataattggt tgcaagaagt ggtgagatgg
     1081 acgtggcgta ttaaattgca gccacattta gaaagtagct ttgtcacaga attaaaaaat
     1141 caagccgatg gcgaagcgat tcatgtcttt ggccaaaatt
//
LOCUS       DAOQMB010000764         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108445_length_1179_cov_17.386121, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000764 DAOQMB010000000
VERSION     DAOQMB010000764.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108445_length_1179_cov_17.386121"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1163
                     /locus_tag="PLY05_13790"
     CDS             228..1163
                     /locus_tag="PLY05_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HNC04410.1"
                     /translation="MKKLLATATLLASSIIASQAQACFFWEDCTYTKTKYPMVLAPGV
                     LGFDKLVGFVDYWYGIPSAVSYSGGKVYTTSASAFNSSEARGEQLLAQVQNILAISGA
                     AKVNLMGHSHGGFSVRYVATAIPSRVASVTTIHSPVKGVKGAAILTGTDGTVAGDVVQ
                     TVGTAVSNFITFLAGNSYKEDFMASMKSLDTAGAAAFNAKHPNGVPTTACGEGAYTTN
                     GIKNYSWSGTSAVTNVLDPLDTFFGVMSLVSTSEPNDGLVGKCVSHFGKVIRDDYPHN
                     HGDAINQLFGVRGLFTPDPKDVYRQHANRLKVAGV"
BASE COUNT          362 a          206 c          232 g          379 t
ORIGIN      
        1 tctgatttga tatgccattt gtcaggcatt taccgtttat cggggtgtcg tcaattacca
       61 gtctgtgtcg cgtattcaca atttttttga cgctaatttc cataaaataa accacataaa
      121 caaaacgaat atcttgcaaa taaaaaatag tgtttttact ctaaaaatta attaaaaatt
      181 taaattaaaa actaaatgcc tgtgaaggta aaccgaggaa aacaaccatg aaaaagttac
      241 ttgctaccgc aactttatta gccagctcta taattgccag tcaagcacaa gcttgttttt
      301 tttgggaaga ttgtacttat acaaaaacta aatatccaat ggtcttagca ccaggcgtct
      361 taggttttga taaattagtt ggttttgtgg attattggta cggtattcct tctgcagtta
      421 gctacagtgg tggcaaagtt tacacaactt cagcttcagc gtttaatagt tctgaagcac
      481 gtggtgaaca attattagcc caagtacaaa atattttagc aatcagtggt gcagccaaag
      541 ttaacttaat gggtcacagc cacggtggtt tttctgttcg ttatgtagca acggctattc
      601 ctagtagagt tgcttcggta accactattc atagcccagt aaaaggcgtt aaaggtgctg
      661 ctattttaac aggtacagac ggtacagttg caggtgatgt agtacaaact gtgggtacgg
      721 cagtttctaa ctttattact ttcttagctg gtaatagcta taaagaagat tttatggcta
      781 gcatgaaatc tttagataca gcaggcgcag ccgcatttaa tgccaaacat ccaaatggtg
      841 taccgacaac agcttgtgga gaaggtgctt atacaactaa tggtattaaa aactattctt
      901 ggagtggtac ttctgctgta acgaatgtgt tagatccatt agatactttc tttggtgtaa
      961 tgtctttagt ttctacttct gagccaaatg acggcttagt aggtaaatgt gttagccatt
     1021 ttggtaaagt gatccgtgat gattacccac ataaccacgg tgatgcaatt aatcaattat
     1081 tcggcgtacg tggtttgttt acaccagatc caaaagatgt ttatcgtcaa cacgctaacc
     1141 gtttaaaagt agcaggcgta taatttaaaa cacttttta
//
LOCUS       DAOQMB010000765         1178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108620_length_1178_cov_13.984862, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000765 DAOQMB010000000
VERSION     DAOQMB010000765.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108620_length_1178_cov_13.984862"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="PLY05_13795"
     CDS             <1..134
                     /locus_tag="PLY05_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HNC04411.1"
                     /translation="AARVSDYTAFMYLGKLIEHGDTDTLFTNPTNKQTEDYITGRYG"
     gene            241..966
                     /gene="phoU"
                     /locus_tag="PLY05_13800"
     CDS             241..966
                     /gene="phoU"
                     /locus_tag="PLY05_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016540822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNC04412.1"
                     /translation="MPANKEDYGHHISQKFNAELEDIRTHFLAMGGLVEKQVTDAIHA
                     LLDADSVLAIAVQADDKKINAMETSLDDALVKVLARRQPAASDLRMIIAISKAIIDLE
                     RIGDEAAKIARYAQVLCEEGESPRGYVETRHIGNQIRVMLHDTLDAFARLDVQQALRV
                     MQSDREVDLEYKTATRALMTYMMEDPRYIARVLNVMWVLRSLERIGDHARNVAEQLVY
                     LVQGTDIRHISDEALKQRIDGQA"
     gene            997..>1178
                     /locus_tag="PLY05_13805"
     CDS             997..>1178
                     /locus_tag="PLY05_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNC04413.1"
                     /translation="MTDKTLLIVAHTPSINTEAVLKAILTGANHSDIQDIDVVYKPAL
                     QATADDIRACQAIILFT"
BASE COUNT          350 a          194 c          277 g          357 t
ORIGIN      
        1 aggcagcgcg tgtgtctgat tacacagcct ttatgtactt gggtaaattg attgagcatg
       61 gtgatacaga tactttgttt actaatccaa ccaataaaca aactgaagat tatattactg
      121 gccgttatgg ttaatgtggt gtcttttttg ctaggtaatt atcgttaatt aaagcgcagt
      181 ttctttacta gggcgcgtgt tatgccctag taaagaacaa caatatcaga ggacaagact
      241 atgcctgcta ataaagagga ttatggccac catatctcgc aaaagtttaa tgctgaatta
      301 gaagatattc gtacccattt tttagcaatg ggtggtttgg tcgaaaaaca agtgacggat
      361 gcgattcatg ccttattaga tgcagattcg gtattagcaa tagctgtaca agctgacgat
      421 aaaaaaatca atgcgatgga aactagcttg gacgatgctt tagtaaaagt gttagcacgt
      481 cgccagcctg cagcttctga tttacgcatg attattgcta ttagcaaagc gattattgat
      541 ttagagcgta ttggtgatga agcggccaaa attgcgcgtt atgcccaagt tttatgtgaa
      601 gagggtgagt caccacgcgg ttatgtagaa actcgtcata ttggtaatca aattcgtgtc
      661 atgttgcatg atactttaga tgcgtttgcg cgtttagatg tacagcaagc tttaagagtg
      721 atgcagtctg atcgtgaagt cgatttagaa tacaaaacgg caacgcgtgc cttaatgact
      781 tatatgatgg aagatccgcg ttatattgct cgtgtgttga atgtgatgtg ggtattacgt
      841 agtttagagc ggattggcga tcatgcgcgt aatgtggccg aacaattagt ctatttggtg
      901 caaggtacag atattcgtca tattagtgat gaagccttaa aacaacggat tgatggtcag
      961 gcgtaaaggc gagattaaaa acggttgact aattttatga ccgataaaac cctgttaatt
     1021 gtggcacata cgccttcgat caataccgaa gcagtgttaa aagcaatatt aacaggggct
     1081 aatcattccg atattcaaga tattgatgtg gtttacaagc cagctttgca agccacagca
     1141 gatgatattc gagcttgtca ggcaattatt ttatttac
//
LOCUS       DAOQMB010000766         1177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_108823_length_1177_cov_5.171123, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000766 DAOQMB010000000
VERSION     DAOQMB010000766.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108823_length_1177_cov_5.171123"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1177)
                     /locus_tag="PLY05_13810"
     CDS             complement(<1..>1177)
                     /locus_tag="PLY05_13810"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HNC04414.1"
                     /translation="PFNQRLDGMFWNEDDEFDLEHHFRHIALPEPGRIRELLTYVSQE
                     HSALLDRAKPLWECHLIEGIEGDRFALYFKMHHAVIDGIAGMRIAQKSLSPDPDAKAA
                     TPLWAIKRDRRPKEEVKALANFAETVNRLRHQATSVPKVAKELYQAIKASYEKDPDYV
                     SVFQAPRSILNQRVSSSRRFAAQSYSLPRIKAIGKKLGVTINDVVMAMCAGALRTYLL
                     DLNALPKKPLIAMVPMSLRRDDSDAGNQITMILANLGTHLADPLARADIIHRSVQNAK
                     DRFCRMTQSEIMAYSALVYSIAGLNLLTGIAPKLQAFNVVISNVPGPKEPMYWNGALL
                     DGFYPVSIVLDGMALNITLTSYVDRLEFGLIACRKALPSMQRLLAYLEDSLNELEQIA
                     "
BASE COUNT          360 a          246 c          248 g          323 t
ORIGIN      
        1 cagcaatttg ctcaagttcg ttgagagagt cttctaaata agctaacaag cgttgcatac
       61 tcggcaaggc tttgcgacaa gcaattaaac caaactccaa acggtcaaca taactggtta
      121 aggtaatgtt gagagccatg ccatctaaaa cgatagaaac gggataaaaa ccatctaaca
      181 atgcgccatt ccagtacatg ggttctttag ggccgggtac gttagaaatt acaacgttga
      241 atgcttgtag tttgggcgca atgcctgtta ataaattaag accagcaatg ctataaacca
      301 aggcactata ggccataatt tcactttgag tcatgcgaca aaagcgatct ttagcatttt
      361 gtaccgagcg atgaataata tcggcacgag cgagtgggtc agcgagatgt gtccctaaat
      421 tggccaaaat catggtaatt tgattgccag catcactatc atcacgacgt aacgacatgg
      481 ggaccatagc aattaacggt tttttaggta aagcattcag gtctaataaa taggtacgta
      541 atgcacctgc gcacattgcc atcactacat cattaatggt cacacccaac tttttaccaa
      601 tcgctttgat gcgtggtagg ctataagatt gcgccgcaaa acgacgcgag ctactaacgc
      661 gttgatttaa gatgctgcgt ggtgcttgaa acacagacac ataatctggg tctttttcgt
      721 aagatgcttt aatggcttga tagagttctt tggcgacttt aggcacgctg gtcgcttgat
      781 gacgtaaacg attcactgtt tcagcaaaat tagctaatgc tttcacttct tctttagggc
      841 ggcgatcacg tttaatcgcc cataaaggcg ttgctgcttt tgcatcaggg tcaggagata
      901 aggatttttg tgcaatccgc atacctgcaa tgccatcaat caccgcatga tgcattttaa
      961 aatataaggc aaaacgatca ccttcaatgc cttcaattaa atgacattcc cagagtggtt
     1021 tagcacgatc taataaggca ctatgctctt gtgaaacata agtgagtaat tcacgaatac
     1081 gacctggttc gggcaaggca atatggcgaa aatgatgttc taaatcaaat tcatcatctt
     1141 cgttccaaaa cataccatct aaacgttgat taaatgg
//
LOCUS       DAOQMB010000767         1176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_109041_length_1176_cov_3.979483, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000767 DAOQMB010000000
VERSION     DAOQMB010000767.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109041_length_1176_cov_3.979483"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="PLY05_13815"
     CDS             <1..155
                     /locus_tag="PLY05_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNC04415.1"
                     /translation="GHPNRYKIKLRSIGYRLVYEVIDEQVVVLVIAVGRRENDAVYQA
                     ADLRHF"
     gene            complement(296..1144)
                     /gene="fghA"
                     /locus_tag="PLY05_13820"
     CDS             complement(296..1144)
                     /gene="fghA"
                     /locus_tag="PLY05_13820"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002115889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HNC04416.1"
                     /translation="MMTAFETRSTHACFGGVQGFYQHQSSVIGLPMRFAVYQPPQAQQ
                     GKVPVLFYLAGLTCTEETFAIKAGAQRLAAEYGVMIVTMDTSPRGAGITGEADAWDFG
                     VGAGFYVDATQEPWSKNYRMESYVTQELRELIVRHFPANESKVGIFGHSMGGHGALVL
                     ALRHPESYQSVSAFAPIAAPSQCPWGQKAFSHYLGDNQQAWRAYDATALIEDGKKAPP
                     ILIDQGLNDQFLANQLYPELLEAACQKSNQPLTLRRHLNYDHGYYFISSFMADHLAHH
                     VRCFMV"
BASE COUNT          348 a          263 c          253 g          312 t
ORIGIN      
        1 cgggacatcc gaatcgttat aaaatcaaat tgcgaagtat tggttatcgt ttggtttatg
       61 aagtaattga tgagcaagtg gtggtcttag tgattgctgt tggtaggcgt gaaaatgatg
      121 cggtatatca ggcagcagat ttgaggcatt tttagctaag tcaatagatc agctaaattg
      181 gagtgtgttg aggtcaatgc tatgatttta tcaatttcaa ctctaatctt gtagttgtgg
      241 gttaagggac gaaacccaca acatataagt aaagttaata gtgataatct tgagcttaaa
      301 ccataaaaca ccgcacatga tgcgccaaat gatccgccat aaagctacta ataaaataat
      361 agccgtggtc ataattcaga tgacgacgca aagttaaagg ctgattgctc ttttggcagg
      421 cggcttccaa taactcagga tacagttgat tagccaaaaa ctggtcgttt aaaccttggt
      481 caatcaaaat gggtggtgct tttttgccat cttcaattaa cgctgttgca tcataagccc
      541 gccatgcttg ttgattatca cccaaataat gactaaaggc tttttgtccc cacgggcatt
      601 ggctcggtgc agcaattggc gcaaaagcgg acacggattg atatgactca ggatggcgta
      661 aggccaacac caaagcacca tgtccaccca tcgaatggcc aaaaataccc actttgcttt
      721 cgttagcagg aaaatgccta acaattaact cacgcaattc ttgagtcaca taactttcca
      781 tgcggtaatt ttttgaccac ggctcttgtg tcgcatcaac ataaaagcca gcacccacac
      841 caaaatccca cgcatcggct tcgcctgtaa tgccagcacc acgtggactg gtgtccatcg
      901 tgacaatcat tacgccatat tcggccgcca aacgttgtgc acccgcttta atggcaaagg
      961 tttcttcggt gcaggttaaa cctgctaaat aaaacaacac agggacttta ccctgttgtg
     1021 cttgtggtgg ctgatacacc gcaaagcgca tcggtaagcc gataacactg gattgatgtt
     1081 gataaaaacc ttgcacacca ccaaagcagg catgtgtgct gcgtgtttca aaagccgtca
     1141 tcattaaaac accactaccg agcgaatcga ttcacc
//
LOCUS       DAOQMB010000768         1174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_109374_length_1174_cov_6.744415, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000768 DAOQMB010000000
VERSION     DAOQMB010000768.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109374_length_1174_cov_6.744415"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..>1174)
                     /gene="terL"
                     /locus_tag="PLY05_13825"
     CDS             complement(38..>1174)
                     /gene="terL"
                     /locus_tag="PLY05_13825"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HNC04417.1"
                     /translation="LGVRANLKLQRLYDSAIYQDIFPDTKISRIGAIGADAIRNRELL
                     EYQNCLGYFRNTTVGGSITGEGLDLGVIDDPIKGRKEANSKTVRDGVWDWFTDDFFTR
                     FSENAGLLCILTRWHIDDPIGRLIQKYPDVKVLSYPAIATNDEKNRKEGEALFPEHKS
                     LDFLLERKSLMDTTSWLSIYQQTPIIVGGELIKGEWFKRYTTLPKLEYKKIFVDTAQK
                     KGKHNDYTVFECWGYADGNIYLIDLVRKKMEAPELRRTANDFWNKHAQSDNGRLREML
                     IEDKASGTGLIQDIKAHDKIPIKAIQRNTDKLTRVQDVLTYIENGYVFIPENASFVSD
                     FIAECEAFTADDAHDHDDQIDPLCDAIDNMIAKRGVTELRVRRL"
BASE COUNT          324 a          265 c          186 g          399 t
ORIGIN      
        1 tttttttgct ctttccaaaa ctgccaccat tttttactca tagtcgtctt actcttagtt
       61 ctgttacgcc gcgcttagct atcatattat caatagcatc gcacaatggg tctatttggt
      121 catcgtggtc atgtgcgtca tcagcggtaa atgcctcaca ttctgctata aaatcgctca
      181 caaaactagc attttcagga atgaaaacat aaccattctc aatatatgtt agcacgtctt
      241 gtacacgggt caatttatcc gtatttctct gtatagcttt aatcggtatt ttatcatgcg
      301 ctttgatgtc ttgtattaaa cctgtgccgc ttgccttgtc ttctatcaac atctcacgca
      361 atcttccgtt gtcactttgc gcgtgtttat tccaaaagtc gttggctgtg cgtctaagct
      421 caggtgcttc cattttttta cgcaccaaat caattaagta gatattgcca tcggcatatc
      481 cccaacattc aaaaactgta tagtcattat gcttgccttt tttttgtgct gtatctacaa
      541 aaatcttttt atactcaagt tttggcagtg ttgtgtatcg cttaaaccac tcacctttga
      601 ttaactcacc accaacaata attggagttt gttgataaat actaagccat gatgttgtat
      661 ccatcaaaga cttgcgctca agcagaaaat caaggctttt gtgttcaggg aataatgctt
      721 caccctcttt tctatttttt tcgtcattgg tggcgattgc tggataagat aaaaccttaa
      781 cgtcaggata tttttgaata agtcggccaa tcgggtcgtc aatgtgccac cgtgttaata
      841 tgcacagtaa ccctgcattt tcgctaaacc gcgtaaaaaa atcatctgta aaccaatccc
      901 aaacaccatc acgaacagtt ttagaattag cttctttgcg cccttttatc gggtcatcaa
      961 ttacacctaa atctaatccc tcaccagtga ttgaaccgcc aacagttgta ttacggaaat
     1021 agcctaaaca attttgatat tcaagcaact ctctgtttct aattgcatca gcaccaatcg
     1081 caccaatacg gcttattttt gtatcaggaa aaatatcttg ataaatagca ctatcatata
     1141 atcgctgtaa tttaaggttt gcacgaacac ctaa
//
LOCUS       DAOQMB010000769         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_109933_length_1171_cov_30.407706, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000769 DAOQMB010000000
VERSION     DAOQMB010000769.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109933_length_1171_cov_30.407706"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..>1171
                     /locus_tag="PLY05_13830"
     CDS             208..>1171
                     /locus_tag="PLY05_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004278529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HNC04418.1"
                     /translation="MFYLGIDVSKAKLDCCLLLDNTTFKRKDKSIANNHKGFNELLAW
                     IQKQSIELSNVHIIMEATGVYHEQAAYALHDAGIKLSLANPAYVRHFAKGLAIKTKND
                     GMDSFVLARYGLSTQPSSWIPAPIEARMLKALLHRREAIAQDLQREHNRQEKAQVACS
                     PEKIQHSIETSIAFLEQQLKKLQDDIDDHIDKHPKLKQDVKLLESIPAVGERVGNTML
                     SVLHNHHFESAEQVAAYLGLVPVEYQSGTSVFRRSHLSKTGSASVRATLYMAAVVATK
                     YNPHIKALYERLLAKGKCKMLAIGAAMRKLVHLCFGVWKNQTPYQ"
BASE COUNT          344 a          230 c          249 g          348 t
ORIGIN      
        1 ttcgacttgt atcttaactc aagtaatgat tagctatcat tacttaacgt gaagagagct
       61 tctggcttcg agtaaagcac agactttgta gcgaagatta agcccttcac gtttcttatc
      121 acccttccga cactgaacgg taaccaaggg caattgaccc tgtatgtaca aggctagtgg
      181 gtgatttggt gagttgagga ggttatcatg ttttatctgg gtattgatgt atctaaagcc
      241 aaattagatt gctgtttatt attagacaac accaccttta aacgtaaaga caaaagtatt
      301 gccaataatc ataaaggttt caatgagtta ttggcatgga tacaaaaaca gtccattgaa
      361 ttaagcaatg tgcatatcat catggaagcc acaggtgttt accatgagca agctgcttac
      421 gctttgcatg atgcagggat taagctgtca ttagccaatc ctgcttatgt tcgtcacttt
      481 gccaaggggt tagccattaa aaccaaaaat gatggcatgg atagctttgt tttagctcgt
      541 tatggcttat ctactcagcc ctcatcatgg attcctgccc cgatagaagc acgtatgtta
      601 aaggccttac tccatcgtcg cgaggcaatt gctcaagatt tacaacgtga gcataatcgc
      661 caagaaaagg cacaagttgc ttgctcccct gaaaagattc aacactctat tgaaaccagt
      721 attgcttttt tagagcagca gctaaaaaaa ctacaggatg atattgatga ccatattgat
      781 aaacatccta agttaaaaca agatgttaaa ttgcttgaaa gtattcctgc ggtgggtgag
      841 cgtgtgggta atacaatgtt atctgtcttg cacaatcacc actttgaatc ggctgaacaa
      901 gtggcagctt atttgggctt agtacctgtt gagtatcaat cgggtacttc tgtattcagg
      961 cgatctcatt tatccaaaac aggctcggct tctgttcgtg ctacgctgta tatggcggct
     1021 gtggtagcaa ctaaatacaa cccacatatt aaagcacttt atgagcggtt actggctaaa
     1081 ggtaagtgca aaatgttagc cattggtgca gctatgcgta aattggttca cttatgcttt
     1141 ggcgtttgga agaaccaaac gccttatcaa a
//
LOCUS       DAOQMB010000770         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_109935_length_1171_cov_16.172043, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000770 DAOQMB010000000
VERSION     DAOQMB010000770.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109935_length_1171_cov_16.172043"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PLY05_13835"
     CDS             complement(<1..440)
                     /locus_tag="PLY05_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04419.1"
                     /translation="MIFNIVPASVGQDYRQLLSSDSQPLRVVAVDIAAETGLTFNEQE
                     QILCGQPLTVGQYALVIYYQLIIENQYSTIKTSHSQLIINPDPRSLWKNIPSNNQEIY
                     AKPDSRSQSLHNAHYKILAASQRGRSHAHKGQFREDDFFIAQGE"
     gene            complement(443..>1171)
                     /locus_tag="PLY05_13840"
     CDS             complement(443..>1171)
                     /locus_tag="PLY05_13840"
                     /inference="COORDINATES: protein motif:HMM:NF026953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerY-C metal binding domain-containing protein"
                     /protein_id="HNC04420.1"
                     /translation="IVYLMTDGKPTDDIKDAVYRWQKNYQQKAELIAIGVGQYADLEA
                     LRKLTPNVVHLNAQSDEDFKKFIDWVSQSVVAQSRSVSANTGGVSLAKLDDSIMSQIG
                     DLGLAAKVDEDYVILSGMCQRYRLPYLIKYERMPMRFDAGQYQVDFGRYHMAGVLALE
                     KDYYDLCDNSMATPTINTDMLIGGAGCPHCGHHYAIAACGNCNQLFCIDGEGEANCPH
                     CQYENNFVMASADSDGFDISRARG"
BASE COUNT          338 a          270 c          213 g          350 t
ORIGIN      
        1 ttttcacctt gagcaataaa aaaatcatct tctctaaatt ggcctttatg ggcgtgagag
       61 cggccacgtt gactggcagc gagtatttta tagtgagcat tgtgcaaaga ttgactacgg
      121 ctatcaggct tggcatatat ttcttggtta ttggaaggaa tatttttcca taaagagcga
      181 gggtctggat taatgataag ttggctgtga ctggttttaa tggtgctata ttgattttcg
      241 ataattaatt gataataaat aaccagagca tactgcccga ctgttaatgg ttggccacac
      301 aatatttgtt cttgctcatt aaacgttaag cccgtttccg cggcaatatc caccgccacc
      361 acgcgcagtg gttggctatc actgcttaat aattgtcgat agtcttgtcc aaccgaggca
      421 ggcactatgt taaaaatcat gcttaacctc gtgcgcgact aatatcaaaa ccatcactat
      481 cggcactagc cataacaaag ttattttcat actgacaatg agggcaattg gcttcccctt
      541 caccatcaat acaaaacagt tgattacaat taccacaagc ggcaatcgca taatgatggc
      601 cacaatgcgg acaacctgcc ccaccaatta acatatcggt attaatggtt ggtgtggcca
      661 tgctgttatc acacaagtca taataatctt tttctaaggc taacacccct gccatatgat
      721 aacgaccaaa atcgacttga tactggcctg catcaaatct catcggcatt cgttcatatt
      781 taatcaaata gggcaaacga taacgctgac acatgcccga taaaatcaca taatcttcgt
      841 caactttagc cgccaagcct aaatcgccaa tttgcgacat aattgagtcg tctaatttgg
      901 ctaaactcac gccacctgta ttcgctgaca cgcttcgact ttgtgccacc actgactgac
      961 tgacccaatc aataaacttt ttaaaatctt catcactttg tgcatttaaa tgcacgacat
     1021 ttggcgtgag tttacgcaac gcttctaaat cggcatattg ccccacacct atggcaatta
     1081 gttctgcttt ttgctgatag tttttttgcc acctatagac cgcatcttta atatcatcgg
     1141 ttggtttacc atcggtcatt aaatagacaa t
//
LOCUS       DAOQMB010000771         1171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_109973_length_1171_cov_3.678315, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000771 DAOQMB010000000
VERSION     DAOQMB010000771.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109973_length_1171_cov_3.678315"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(130..903)
                     /locus_tag="PLY05_13845"
     CDS             complement(130..903)
                     /locus_tag="PLY05_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04421.1"
                     /translation="MSDLFLPVKVGLGQYMTRFKQSLIVIHNDSAAVQDFANRALPKS
                     MVFAPSRMIDAIEDILSAWRKNTNDAIKPQSTAFLPMIAVAIARDYTPASPSQGMMLG
                     DAIDVKLPNYPDERRLKMELIRGQLRVQVVVIAPDDSSAKSLMMRFCHFVRRYQNRGF
                     DAVYNLAGQNEPWFNTWDSQDVYPMSVPIAQTNLAIQKVDLLLNIAIPMVYGAASGQP
                     NSGIGTGTPNDPFGLPVLTQINLWQFNLGQINAPDKVIT"
     gene            complement(906..>1171)
                     /locus_tag="PLY05_13850"
     CDS             complement(906..>1171)
                     /locus_tag="PLY05_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04422.1"
                     /translation="CHVLDSVGALSSQKQQLEQMIDELMTKQSQPTRGSRSPIFIDER
                     KIARFDNAQSFNAMDLAGVELIDDDEVKAKRAELQKIKRYFGM"
BASE COUNT          310 a          275 c          222 g          364 t
ORIGIN      
        1 aggggtcaaa cttttcagca ttatcaatca tcaaaaagcc tgatgcttcg tccattgcag
       61 caaaaacaca tagcatatcg ccatgataac cctgtgcaat tgccctaatt tttaatacac
      121 catcaatgct catgttatca ccttgtcggg ggcgttaatt tgccctaagt taaattgcca
      181 caagttaatt tgtgttaaaa caggtaagcc aaaggggtcg ttaggcgttc cagtgcctat
      241 gccgctattc ggttgtccac ttgctgcacc ataaaccatt ggaatagcaa tgtttaacaa
      301 taaatccact ttttgaatgg ctaggttagt ttgtgcaatc ggtacagaca tcggataaac
      361 gtcttggcta tcccatgtgt taaaccacgg ctcattttgc cctgctaaat tgtaaaccgc
      421 atcaaagccg cgattttgat accgtctgac aaagtgacaa aagcgcatca ttaaactttt
      481 tgcgctacta tcatcgggtg caattaccac cacctgaacc cttaattgcc ctctaataag
      541 ctccattttt agtctgcgct cgtcaggata attgggcaat ttaacgtcta tcgcatcgcc
      601 taacatcatg ccttgacttg gtgatgctgg cgtataatct cgtgcaatgg caaccgctat
      661 catcggtaaa aatgccgtac tctgtggctt tattgcgtca ttggtatttt tacgccatgc
      721 gcttaaaata tcttcgattg cgtcaatcat gcgtgaggga gcaaatacca tactcttagg
      781 caaggctctg ttagcaaaat cttgtactgc cgcgctgtcg ttatgaatca caatcaatga
      841 ttgtttaaag cgcgtcatgt actggcctag tcccacttta acaggcaaaa ataagtcact
      901 catgcttaca ttccaaagta gcgtttgatt ttttgcaatt cagcacgttt ggcttttact
      961 tcgtcatcat caatcaattc tacccctgct aaatccattg cattaaaaga ctgtgcattg
     1021 tcaaaacgcg ctatctttct ttcatcaatg aatatcgggc tacggctgcc gcgtgttggc
     1081 tgtgactgtt tggtcattag ctcgtcaatc atttgctcta attgctgttt ttgactgctt
     1141 aacgcgccta cgctatctaa aacatggcaa t
//
LOCUS       DAOQMB010000772         1170 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_110128_length_1170_cov_7.616143, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000772 DAOQMB010000000
VERSION     DAOQMB010000772.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110128_length_1170_cov_7.616143"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            266..625
                     /locus_tag="PLY05_13855"
     CDS             266..625
                     /locus_tag="PLY05_13855"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNC04423.1"
                     /translation="MADESARHNVLTGNFATVLHMHLKSTSCQSYALRMKVKVGQNYF
                     YPDVLVDCSDVDGYSTETPTLIIEVLSKSTRKLDETTKWLLYLQIPTLLEYILVEQDC
                     GLKSRGVVRVGSPCAIF"
     gene            610..738
                     /locus_tag="PLY05_13860"
     CDS             610..738
                     /locus_tag="PLY05_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04424.1"
                     /translation="MRYFLGDQVVLESVGLTLNVEDIYERVNVNNEDMRQWLEAKL"
BASE COUNT          339 a          214 c          253 g          364 t
ORIGIN      
        1 tgcgtatggc cgaaaaatgg ttaagcccta atttggggta tgagcctgag taagttgtat
       61 cccaaaaggc ttgcagtata tgaccccttt agagcaatat caaacactcc atgataatac
      121 tcaatttatc ttgcgcatga attagcttta gaggtgtaca acgcctattg ccaaaaaagt
      181 aaattttgtt agcgaacaag attatctgga aggcgagcta gttacaacgt ttaaaaatga
      241 actgatagat ggtcatattt atccaatggc cgatgaaagt gctaggcata atgttttaac
      301 tggaaatttt gcaaccgtcc tacatatgca tttgaagagc acgtcatgtc agtcttatgc
      361 tttgagaatg aaagtcaaag tcggtcaaaa ttatttttat cccgatgtct tggtggactg
      421 ttcggatgtg gacggttatt ctaccgaaac gccgacttta attatcgaag tgttatctaa
      481 atcgactcga aagctggatg aaaccactaa atggctgttg tatttgcaaa ttcctacgtt
      541 acttgagtat attttggttg agcaagattg cggattgaag tcgcgcggtg tagtgagggt
      601 tggcagccca tgcgctattt tttaggtgat caggttgtgc ttgagtcggt aggtttgacg
      661 cttaacgttg aagatattta tgaacgtgtt aatgttaata atgaagatat gaggcagtgg
      721 ttagaggcta aattgtagag tgggttgacg taagaaatcc acagtttgag gatgatggat
      781 ttcataaatc tacacatgct tactactttc actcaatctc aatatccaaa tatttcaaaa
      841 aatatcttgc ggcaacagat agtggtttat ctttacgcca gaccactgac cagtgccgca
      901 taattggaaa accttctaca tgtagtatag ttaacggtac atcagatgat tgcgtagcat
      961 tctttggcca cagtggagga taaaccgcta actcggtgta atggtcaagg ctttctgtaa
     1021 tcatgatcgt gtcaaattat ggttagctat acgctcctta taccaaaaca cgtcttgttt
     1081 tgacactacg gtttttcttt cataaaccca cacttctcac gcataccgcg tcaataactc
     1141 tttaacttcg ctcatccgtt gggcagcatc
//
LOCUS       DAOQMB010000773         1169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_110319_length_1169_cov_12.470377, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000773 DAOQMB010000000
VERSION     DAOQMB010000773.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110319_length_1169_cov_12.470377"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1169
                     /locus_tag="PLY05_13865"
     CDS             <1..>1169
                     /locus_tag="PLY05_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein"
                     /protein_id="HNC04425.1"
                     /translation="GYVILPVVIPAGVEPHEALNDNKTYKVVWQVLQALRSHDDRFDA
                     MINKMDLDLNANMLSKMEVIAITDKINPKAKLTKGKTSRQQAGKGSFGIGKKVDNNAP
                     EQHEMEFEVGEIERAIYAKVVQKCGNRRHWEDWANDIAKIANTHISRIQAILDNPDNT
                     AEIAAFTAFAEELRDDLNNAITDGEIVEMLAQHLITKPVFDALFSDYSFAKFNPMSIA
                     MQDVLDKLHEHQLHKETDTLQRFYDSVQERAKGIDSATGKQKIVVELYDKFFRNAFPK
                     MTERLGIVYTPVEVVDFIIHSVNDALKNEFGQTLGDKNVHIIDPFTGTGTFITRLLQS
                     GLISDAQLPYKYKNEIHANELVLLAYYIAAINIEAVYHDIILDDYAPFNGICLTDT"
BASE COUNT          358 a          223 c          256 g          332 t
ORIGIN      
        1 ggttatgtga ttttgcctgt ggtgattcct gctggtgtag agccacacga agcccttaac
       61 gacaacaaaa cctataaagt ggtgtggcaa gtcttacagg cgttacgttc tcacgatgac
      121 cgttttgatg ccatgattaa caaaatggat ttagacctta acgccaatat gttgtctaaa
      181 atggaagtga ttgccattac tgacaaaatc aatccaaaag ccaaactcac caaaggtaaa
      241 accagccgtc aacaagcagg caaaggcagt tttggcattg gtaaaaaagt tgacaataac
      301 gcccccgaac aacacgaaat ggagtttgaa gtcggtgaaa ttgaacgtgc gatttatgcc
      361 aaagtggtac aaaaatgtgg taatcgtcgc cattgggaag attgggcaaa tgacattgcc
      421 aaaattgcca atactcacat tagccgtatt caagccattt tagataatcc cgataatact
      481 gccgaaatag ccgcgtttac tgcctttgcc gaagaattgc gtgatgacct caataacgcc
      541 attaccgatg gcgaaattgt cgagatgttg gcacagcatt taattactaa acctgtgttt
      601 gatgccttat ttagtgatta cagttttgcc aagtttaatc cgatgtcgat agccatgcag
      661 gacgtgctag acaagttaca tgaacatcaa ctgcacaaag aaaccgacac cctacaacgc
      721 ttttatgaca gtgtgcaaga acgcgctaaa ggcattgata gtgcgacagg taagcaaaaa
      781 attgtcgtgg agctgtacga taagtttttt agaaatgctt ttcctaaaat gaccgagcgt
      841 ttaggcattg tttatacgcc tgtggaggtg gtggatttta ttattcatag cgtcaacgat
      901 gccttaaaaa atgagtttgg ccaaacttta ggtgataaga atgtgcatat tattgacccg
      961 tttacaggca caggcacgtt tattacgcgc ttgctgcaaa gcggtttaat tagtgatgct
     1021 caattaccct ataaatataa aaatgagatt catgccaatg agttggtgtt actggcttat
     1081 tacattgctg ccattaacat tgaggcggtt tatcacgata ttattttgga tgattacgcg
     1141 ccgtttaatg gtatttgttt gactgatac
//
LOCUS       DAOQMB010000774         1167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_110770_length_1167_cov_2.844424, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000774 DAOQMB010000000
VERSION     DAOQMB010000774.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110770_length_1167_cov_2.844424"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1167
                     /locus_tag="PLY05_13870"
     CDS             <1..>1167
                     /locus_tag="PLY05_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HNC04426.1"
                     /translation="LVFVYGKVNAGKSSLGNLVAMHPASKTKPQFFRYDEAGKARHQA
                     ELEELKAIEGFETKETEATSSIQGFRLPALSWIDTPGLHSMTPVNGKLAQDYIDAADI
                     VLYITSSDSPGRSTDAQEITDLITLRAKKVCVLLTKSDVSEEDEVNGELIAVLKAKSP
                     QVRAEQEKWVKQQLKDYLKQEANRIGDVISISVLLAKNGDWRESNLDMFYQLLNNQAL
                     KHAKTLKQETPRLRFNSLLTQTLQGNQTDAPSLQQIKQLFEQLEKKAYNDGQQLLAAQ
                     QVVWEAIETELYGIISNIVDKHAASKNTTAIKNELETAFPELVKQHLMTEISQHIQQF
                     NDNLPLSLQLNQAINLTDKTESLSYTVSHSGKGAAAGALAGFLLGGPVGAMLGG"
BASE COUNT          393 a          207 c          248 g          319 t
ORIGIN      
        1 gctagtattt gtttatggca aagtaaatgc gggcaaaagt tctttaggta atttggttgc
       61 tatgcaccct gcgagcaaaa caaagccaca gttttttcgt tatgacgaag ctggcaaagc
      121 acgtcatcaa gctgagttag aagagctaaa agccatagag ggctttgaaa caaaagaaac
      181 cgaagcaacc agtagtattc agggctttag attaccagca ttatcttgga ttgatacacc
      241 tggcttgcat tcaatgacac ccgtcaatgg taaacttgcc caagattata ttgatgctgc
      301 tgatattgtg ttgtatatca ctagctccga tagtcctggt cgtagtacag atgctcagga
      361 aattaccgat ttaatcacct tacgtgctaa aaaagtatgt gtattattaa ctaagtcgga
      421 tgtaagcgag gaagatgaag tcaatggcga gcttattgcc gttttaaaag caaaatcacc
      481 acaggtgcgt gccgaacaag aaaaatgggt aaaacaacaa ttaaaagatt atttaaaaca
      541 agaggcgaat agaattggtg atgtgatttc tatttctgtg ttgttggcaa aaaatggaga
      601 ttggcgagag tcaaatttag atatgtttta tcagcttctt aacaatcaag ctttaaagca
      661 tgccaaaacg ttgaaacaag aaacacctcg tttgcgtttt aatagtttac tgacacagac
      721 cttgcaaggt aaccaaacag atgcaccttc gttacaacaa attaagcagt tatttgaaca
      781 attagaaaaa aaggcttaca acgatggtca gcagttatta gccgctcaac aggttgtttg
      841 ggaagcaatt gaaacggagt tatatggtat tatcagtaac attgttgata aacatgcagc
      901 gtcaaaaaat acaacggcca tcaaaaatga attagaaacg gcttttcctg agctagtcaa
      961 acagcatctg atgacagaaa ttagccaaca tattcagcag tttaacgata atttaccttt
     1021 aagcctacag ttaaaccaag ctattaacct tactgataaa acagagtcat tatcttatac
     1081 cgtgagtcat agtggcaaag gcgcagccgc aggtgcattg gcaggattct tactcggtgg
     1141 accagtcggt gcaatgcttg gtgggtt
//
LOCUS       DAOQMB010000775         1166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_110888_length_1166_cov_10.137714, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000775 DAOQMB010000000
VERSION     DAOQMB010000775.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110888_length_1166_cov_10.137714"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PLY05_13875"
     CDS             <1..1159
                     /locus_tag="PLY05_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04427.1"
                     /translation="IRKKVERPTDKYWYMTVSGGYGIPFLTTNKRSPLKEVGDKQWYQ
                     HGTNLSVKTAFGTNGGGFAFNVGWGHMFNKYIGIDVLHTFAWHPESLAAKIDLDTYYA
                     TQKTGTFSIYVSPHLVMRWDNGKRFGITGKAGLVLPIFGKTTSRAYILDKQGRVIETL
                     AGLPILPLSIPGLLGLEIEIVGKAHTEYHPTVGVSASIGFDVKLNKQLSLFAEMRVQA
                     YTISLRETIFDEFKQTSKINVLGIKLDAPEGMLAIPSNVANVSEAPEFLKHYVYRKEL
                     NENSNTARYGTRSLFAEDGLGGLVRTLTGQTGLPSVDINKPMEEPGQKFNASSLYFNA
                     GLRVNFDHWNKKRSSKRVAQASKSSSKPSKSKKSKSSKSMVDDPDSTINVQ"
BASE COUNT          404 a          208 c          225 g          329 t
ORIGIN      
        1 aattagaaaa aaagtagaaa gacctacaga taaatattgg tacatgacag ttagcggtgg
       61 ttatggtatt ccatttttaa ccactaacaa acgctcacct ttaaaagaag ttggcgataa
      121 acaatggtat caacatggca ctaacttaag tgtaaaaact gcttttggta ccaatggtgg
      181 tggctttgct tttaacgttg gctggggaca catgtttaat aaatacatag gcatcgacgt
      241 gttgcacact ttcgcttggc acccagaatc tttagctgct aaaatagatt tagatactta
      301 ctatgccaca caaaaaacag gtactttcag tatttatgtt tctccacact tagtaatgcg
      361 ttgggacaat ggcaaacgtt tcggcatcac aggaaaagct ggcttagtat tgccaatatt
      421 tggtaaaact acttcaagag cctacatctt agataaacaa ggtcgtgtaa tcgaaacctt
      481 ggcaggtttg ccaatcttac ctttaagcat tcctggatta ttaggcttag aaattgaaat
      541 cgttgggaaa gcacacacag agtatcatcc aactgttggc gtgagtgcgt ctattggctt
      601 cgatgtgaaa ttaaataaac aattgtcatt gtttgcagaa atgcgtgtgc aagcttatac
      661 aatctcatta agagaaacca tctttgatga gtttaagcaa acttcaaaaa ttaatgtatt
      721 aggcattaaa ttagatgcac cagaaggtat gctggcaatt ccatcaaatg tagcaaatgt
      781 aagcgaagca ccagaattct taaaacacta cgtgtataga aaagaactga acgaaaattc
      841 taacacagct cgatatggta cgagaagttt atttgcagaa gatggattag gcggcttagt
      901 acgtacgcta actggccaaa caggtcttcc tagtgttgat atcaacaagc caatggaaga
      961 gcctggtcaa aaattcaatg catcaagttt gtattttaac gcaggtcttc gtgtaaactt
     1021 tgaccattgg aataaaaaac gtagttcaaa acgtgttgct caagcatcaa aatcatcttc
     1081 caaaccttca aaatcaaaaa agtcaaaaag ttcaaagtca atggtggacg atccagatag
     1141 tacaattaat gttcaataat caacta
//
LOCUS       DAOQMB010000776         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_111065_length_1165_cov_9.779279, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000776 DAOQMB010000000
VERSION     DAOQMB010000776.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111065_length_1165_cov_9.779279"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..663)
                     /locus_tag="PLY05_13880"
     CDS             complement(76..663)
                     /locus_tag="PLY05_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HNC04428.1"
                     /translation="MGEITDRQMAVKPSTKDIWLNESAPKGHGRFIARITPSGDRLFY
                     FRYTDSDGKRIRLPLGAYDRDGTTGLTLKAARKKANELSVLYQSGIINLRESLEAEEN
                     ARIAIRDAELARIAKEKADAEAEAKRLNERQTVRALFERWIKLEIVHHKDGGAQVRRI
                     MEKDVLPYLGFMHVEDVKKSHIMPITGFVAQIAIN"
BASE COUNT          346 a          255 c          183 g          381 t
ORIGIN      
        1 attgtaactt gaccaatttg tcgtgcgata ttttgctggg ctataagtct tcatcccttc
       61 attttacaga atccctcaat taatcgctat ttgtgcaaca aagccagtga tcggcataat
      121 atggcttttt ttaacgtctt caacgtgcat aaaacccaaa taaggcaata catctttttc
      181 cataatacga cgtacctgtg ccccaccgtc tttatgatga acaatttcta atttaatcca
      241 acgttcaaat aaggcacgta ctgtttgacg ctcatttaat cttttagctt cagcctcagc
      301 atcagctttt tcttttgcga tacgtgctag ttctgcatca cgaattgcaa tccttgcatt
      361 ttcctcagcc tccaaagact cgcgtaaatt aataatacca ctttgatata agactgataa
      421 ctcatttgct ttcttacgag cagcttttaa ggttaggcct gttgttccat ctcggtcata
      481 agctcctaaa ggtaaacgaa tccttttacc atcagaatcc gtatatctaa agtaaaataa
      541 acgatcaccg cttggggtaa tacgtgcaat aaatcgtcca tgacctttag gcgcagactc
      601 atttaaccaa atatctttag tactaggctt cacagccatc tgcctatctg taatttcacc
      661 catcctaaaa taccccattt tctggtgacc gtttggtgac cgtttgtact tagcttaagt
      721 aaaattctat aggacaacat aggattgtca attttattta tttattataa atcaatatct
      781 taacatttat cacaagacaa cataggactg tataaaacag cattttgaaa gcatggggtg
      841 caaggggtag cgagttcaaa tcccgccgtc ccgaccaata aaatcaaagg gttatgttta
      901 acgacataac ccttttttat tgagtggtta aaaaataggc tgtctggttg ccaactgaga
      961 ttggcttgca gttgataata tcactttatt gattgctatc atttttggac agtacaggct
     1021 tgcctatcaa tctacaatcc actacatcat tcaattgctt aaaaataact taaaatattt
     1081 cacacttttg tcaaaattag ctatataatc cgcgccgcgt taacaacgca gttgagcatc
     1141 gcctccaatc tctatcttaa cgagg
//
LOCUS       DAOQMB010000777         1165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_111067_length_1165_cov_7.743243, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000777 DAOQMB010000000
VERSION     DAOQMB010000777.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111067_length_1165_cov_7.743243"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..1043
                     /gene="nuoH"
                     /locus_tag="PLY05_13885"
     CDS             42..1043
                     /gene="nuoH"
                     /locus_tag="PLY05_13885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNC04429.1"
                     /translation="MFDWLTPQVIDILIAVLQAVIILLVTVVSAALLSFVERRLLAWW
                     QDRYGPNRVGPYGMFQIAADMLKMFFKEDWTPPFADKLTFRLAPAIAMGALLLSMAVI
                     PITPTWGVIDLNIGLLFFFGMAGLAVYSVMFAAWSSNNKYALLGGLRAAAQTLSYEVF
                     MGIALMGVVAQAGSFNLREIVEAQRDMWNIFPQFLGFCTFLLAGIAVTHRHPFDQPEA
                     EQELADGYHIEYSGMKWGMFFVGEYIGVVMVSSLLTTLFFGGWLPLFDFLDFIPAFFW
                     FAGKTAFFIMLFVLVRGSVMRPRYDQVMKFSWLFCLPVTLLNLLVTAAVILAWGSPS"
BASE COUNT          257 a          211 c          289 g          408 t
ORIGIN      
        1 caaggtaatg aatgggtaac agtaacaggg gagggcgcat aatgtttgat tggctcactc
       61 ctcaagtcat tgacatctta attgcggtat tacaagcagt cattatttta ttagtgaccg
      121 tggtaagtgc tgctttattg agttttgttg agcgacgttt attagcatgg tggcaagacc
      181 gttacgggcc aaatcgtgtt gggccttatg gcatgtttca aattgctgcc gatatgctca
      241 aaatgttttt taaagaagac tggacaccac cttttgccga taaattaacc tttaggcttg
      301 cccctgccat tgcgatgggt gcgttattac tatcaatggc agtgattcct attaccccga
      361 catggggtgt tattgacctt aatattggct tgttgttctt ttttggtatg gcaggtttgg
      421 ccgtgtattc ggtcatgttt gccgcgtggt catctaataa taaatatgct ttattgggtg
      481 gattacgggc ggcagcgcaa actttgagtt acgaagtgtt tatgggtatt gcgctcatgg
      541 gcgttgtggc acaagcaggt tcgtttaatt tgcgtgaaat tgtagaagcg cagcgtgata
      601 tgtggaacat ttttccgcag tttttaggct tttgtacttt tttactggca ggcattgcgg
      661 taacgcaccg tcatcccttt gaccaacccg aagccgaaca agaattagct gatggttatc
      721 acattgaata ctcaggtatg aagtggggta tgttctttgt gggtgagtat atcggcgtgg
      781 tcatggtgtc ctcgctatta actaccttgt tttttggtgg ttggttgcca ttatttgatt
      841 tcttagattt tattccagcc ttcttctggt ttgcgggtaa aactgccttt tttatcatgt
      901 tatttgtgtt ggttcgtggt tctgtgatgc gtccacgtta tgaccaagtg atgaaattta
      961 gttggttatt ctgcttacct gtgactttac ttaacctctt agttaccgct gcggtgattt
     1021 tagcgtgggg ttcgccatca tgatttttca agcttataaa tcaaccgtct cgattattaa
     1081 aggggcgggt acgcaattac gcagtatcgc tatggtattt agtcatggct ttcgtaagcg
     1141 tgacaccatt caatacccag aagaa
//
LOCUS       DAOQMB010000778         1164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_111273_length_1164_cov_3.475203, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000778 DAOQMB010000000
VERSION     DAOQMB010000778.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111273_length_1164_cov_3.475203"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..>1164
                     /locus_tag="PLY05_13890"
     CDS             35..>1164
                     /locus_tag="PLY05_13890"
                     /inference="COORDINATES: protein motif:HMM:NF037905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04430.1"
                     /translation="MSVVTCTIEQLKWWLINGDKSRPVTKERLPYAGLERLDSVIWGG
                     EDKADLASHSGQVAKLLYQIRQSDSFNHALEPLSYHDLTQELGNQGFVYENYDALVIE
                     YNKLERKAKQMLMAMQGAADIVKPVGVAVSKPLTKGGVANVIAWFEMDDGQAVGVFFH
                     NPDKTPKKIDPTDEMISYKWVLNKKDITIVVAPENGKDLVVRTVAKRIMKLVDANHDR
                     FLKANSDKAELNLKEQNLNAQVIEKQAELDALNKDIEALEAKKAEKEANKLKVPTGEE
                     LRQQGYNGVISELKKLGWTDDAVTGGVSKSFIGASEKGTVNPDGVLTVAVTSKDGNMM
                     AIVGDAIVSGSVVTIPQYGLTAQTEGSYKKEANTINNAVEAYV"
BASE COUNT          381 a          191 c          295 g          297 t
ORIGIN      
        1 tcaaaagtgt tttgaatatg gctacattgg cctaatgagc gtagtcactt gcacgattga
       61 acagttaaaa tggtggttaa tcaatggtga taagtcacgg cctgtgacta aagagcgttt
      121 gccatacgca ggtttagagc gtttggatag cgtgatttgg ggtggtgaag acaaggcaga
      181 cttagccagt catagcggac aagttgctaa attgttatat cagattcgcc agtctgacag
      241 ctttaaccat gctttagagc ctttgtcata ccacgattta acccaagaat taggcaatca
      301 aggttttgta tatgaaaatt acgatgcttt agtgattgag tacaacaaac tagaacgtaa
      361 ggccaagcaa atgcttatgg cgatgcaagg cgcggcagat attgttaaac ctgtgggcgt
      421 agcggtgagt aagccattaa ctaaaggcgg tgtagccaat gtgattgcat ggtttgagat
      481 ggacgatggt caagcggtag gcgtgttttt tcataatccc gataaaaccc ccaaaaagat
      541 tgaccctaca gacgagatga ttagctacaa gtgggtgctt aataaaaaag atattaccat
      601 tgtagtagca cctgaaaatg gcaaggactt agtagttcgt actgtagcca agcgcattat
      661 gaagttagtt gatgctaacc atgaccgttt tttaaaagcc aatagtgaca aggccgaatt
      721 gaacctaaaa gagcaaaact taaatgctca agtgattgaa aagcaagccg aacttgacgc
      781 gctgaataaa gatattgagg cgttggaggc gaagaaggcg gagaaagagg ctaataagct
      841 aaaagtgcct acaggcgaag aattaagaca acaaggttat aacggtgtta ttagtgagct
      901 taaaaaatta ggctggactg atgatgcagt gacaggtggt gtatcaaaat cgtttatcgg
      961 agcaagtgaa aaaggcactg taaaccccga tggtgttttg acggttgctg tcacttctaa
     1021 agatggcaat atgatggcaa tagtgggtga tgccattgta agcggttcgg ttgtaactat
     1081 tccgcaatat ggtttaacag cacaaacaga aggaagttac aaaaaagaag ccaacacaat
     1141 caataatgca gttgaggctt atgt
//
LOCUS       DAOQMB010000779         1163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_111431_length_1163_cov_7.037906, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000779 DAOQMB010000000
VERSION     DAOQMB010000779.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1163
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111431_length_1163_cov_7.037906"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1103)
                     /locus_tag="PLY05_13895"
     CDS             complement(21..1103)
                     /locus_tag="PLY05_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04431.1"
                     /translation="MMWGSLLNLFNTVNAAQAASPKPQPRPAQSASQAMQTITVYDAH
                     KNAKTLDSMLAKGGEGSVYAVQGRSDVLVKLYHPDVLNKLQPHLQQKITAMLHNKPKN
                     ESLLSWPLIDVYNQQQQWIGYAMRRKQGLKLQFLAHAILYKKHFPHFNRRDIVQVLLS
                     LIEQIKQLHSQQIMIGDYNLNNFLCDPHTKTISLIDCDSYQCQFNGQFFACPVGSPDL
                     TPPEHQNKSFKEVVRNSQSEVFSLAIILSKCLMLGRHPYDMVNGEDPVSNMNSGRFAY
                     GKGNQGIPKGDWYNIWSHMPYQLKSLFIRTFSEGALNPSLRPSLAEWHQALSLYIKEM
                     DKGWHEVAVRPQHAKKSEYRGTHSVS"
BASE COUNT          321 a          242 c          249 g          351 t
ORIGIN      
        1 gataaaaacg ccacacggga ctaactcacc gaatgtgtgc cgcgatactc tgattttttg
       61 gcatgttgcg gtctaaccgc cacttcgtgc cagcctttat ccatttcttt gatatataaa
      121 gatagagctt gatgccattc ggctaaactg ggtcttaatg agggattgag tgcgccttcg
      181 ctaaaggtac gaataaacag acttttaagc tgatagggca tgtggctcca aatgttgtac
      241 caatcgcctt tgggaatacc ttgattacct ttgccatagg caaaacggcc agaattcata
      301 ttacttacag ggtcttcgcc attgaccatg tcataaggat gacggcctaa catcaggcac
      361 ttagacaaaa taatggccaa cgaaaacact tcagactgcg aattacgcac tacttcttta
      421 aaggatttat tttgatgttc gggtggggta aggtcgggac tgcccacagg acaggcaaaa
      481 aattggccat taaattgaca ctgataactg tcgcaatcaa ttaagctaat agttttagtg
      541 tgtgggtcgc acaaaaagtt attcaaatta taatcgccaa tcataatttg ttggctgtgc
      601 aactgcttaa tttgttcaat taggcttaac aacacttgca caatatcgcg gcggttaaaa
      661 tgcggaaaat gttttttata caagatggcg tgtgccaaaa actgtagttt taagccttgt
      721 ttgcgacgca tggcataacc aatccattgt tgttgctggt tatatacatc aatcaatggc
      781 caactgagca aactctcatt tttaggtttg ttgtgtaaca tggcggtaat tttttgttgt
      841 aagtggggtt gtaacttgtt taatacgtcg gggtgataca atttgaccaa cacatcgctg
      901 cgtccttgca cggcatatac actcccttca ccacctttag ccaacatact gtctaaagtt
      961 tttgcgtttt tatgtgcatc ataaaccgtt atcgtttgca tagcttgact cgctgactgt
     1021 gctggacgtg gctgaggctt tggactggcg gcttgtgccg cattaacagt attaaacaaa
     1081 ttcaacaatg aaccccacat catattgttc tcctaaatag cttcccaact attggtaggg
     1141 ggtaattgaa ccgccgaact ggt
//
LOCUS       DAOQMB010000780         1160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_111989_length_1160_cov_11.442534, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000780 DAOQMB010000000
VERSION     DAOQMB010000780.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111989_length_1160_cov_11.442534"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="PLY05_13900"
     CDS             <1..527
                     /locus_tag="PLY05_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04432.1"
                     /translation="GSDYIKEPIHKVFKDKEIKVYKDSERADGQETLVAKEPWYVYNA
                     NYGTSEEKKFVELFSRRFEGLSQKFENIYLIRNEREIKIFDKLGRAFEPDFLLFCKQR
                     DGEQMTFQVFIEPKGEHLKGHDKWKEDFLKEIRTEQKAIKIHTDTYLITAVPFYNYNH
                     ENEFKITLESTLNL"
     gene            544..1101
                     /locus_tag="PLY05_13905"
     CDS             544..1101
                     /locus_tag="PLY05_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HNC04433.1"
                     /translation="MKKLEFFYDIVSPYSYIAATQLGQFDGKAEVVWKPVLLGGVFKA
                     TGNSGPAFTVPAKMPYMFKDLQRLSAYYQIPFKMPANFPANTVTVQRVLCAASDAERP
                     ALTHKLYQAYWGQGLDVTNIELLTELVGADLLAKAQEDSVKNALKANTEEVVARGAFG
                     APTFFVGDEMFFGSDRFFLIQEAVK"
BASE COUNT          402 a          205 c          240 g          313 t
ORIGIN      
        1 aaggttcaga ctatatcaaa gaaccaattc ataaagtttt caaagacaaa gaaattaaag
       61 tttacaaaga cagcgaaaga gcagacggac aggaaacttt agtagccaaa gagccttggt
      121 atgtttacaa tgccaactac ggaacaagtg aagaaaagaa atttgtagaa ctgttttcaa
      181 gacgatttga aggactgagt caaaagtttg aaaacattta cctaatcaga aacgaaagag
      241 aaattaaaat cttcgacaaa ctcggacgag catttgagcc tgacttttta ttgttctgca
      301 agcaacgtga tggagaacaa atgacttttc aggttttcat tgagcctaaa ggagaacatt
      361 taaaaggaca cgacaagtgg aaagaagact ttttgaaaga aatcagaacg gaacaaaagg
      421 ctattaaaat ccacacggac acttatttaa taacagccgt gccgttctac aattataacc
      481 atgaaaacga atttaaaata actttagaaa gtacattaaa cttgtaaaac aaagagaccc
      541 cccatgaaaa agctcgaatt tttttacgat attgttagcc cctatagcta tattgccgcc
      601 acacaattag gtcagtttga cggcaaagcc gaagttgttt ggaagcctgt gttattaggt
      661 ggtgtattta aagccacagg caactcaggg ccagccttta ctgtgcctgc taaaatgcct
      721 tatatgttca aagacttaca acgcttatcg gcttattatc aaattccctt caaaatgcct
      781 gctaactttc ctgcgaatac cgtgactgta caacgcgtat tatgtgcggc tagtgatgca
      841 gagcgtccag cattaactca taaattatac caagcctatt ggggacaagg gttagatgtc
      901 accaatattg agttattaac tgaattagtg ggtgcagatt tattggcaaa agcacaagaa
      961 gacagcgtaa aaaatgcctt aaaagccaat accgaagagg tggtggcgcg tggtgcattt
     1021 ggtgcgccaa ctttttttgt gggtgatgag atgttttttg gttcagaccg tttcttttta
     1081 attcaagagg ctgttaaata atgagctatc aacatcagtg gttaaatggc gaaaagattg
     1141 atttacctgt tggcaaagcc
//
LOCUS       DAOQMB010000781         1158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_112369_length_1158_cov_17.582049, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000781 DAOQMB010000000
VERSION     DAOQMB010000781.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112369_length_1158_cov_17.582049"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLY05_13910"
     CDS             complement(<1..386)
                     /locus_tag="PLY05_13910"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="catalyzes the formation of L-aspartate
                     4-semialdehyde from L-homoserine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNC04434.1"
                     /translation="MKPVKIGIVGLGTVGGGAFNLLKANAAEISRRTGRDIIVSHVGA
                     RRDYPQYDLTGVTVSRDIFAVVNDPEVDIVVEVIGGTTTARELVLQAIANGKHVVTAN
                     KALLAQYGNEIFGVAEAKGVFVAFEAA"
     gene            complement(534..>1158)
                     /locus_tag="PLY05_13915"
     CDS             complement(534..>1158)
                     /locus_tag="PLY05_13915"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HNC04435.1"
                     /translation="AIPDAEITSVKQSVAGLYEIKAKGYGTAYMTADGRYMIQGEIIE
                     LNGAKLTNITEQGMAGHRKELLAAVPLKDMVIFPAANGKAKGIAYVFTDVDCGYCRKI
                     HEEIPVLSQMGIEIRYLAFPRAGYPSPTSQKMDAVWCAKDRNAAMTAMKRNQPVAPAT
                     CQNPIKQQYELGQEIGVRGTPAVFLEDGTQVGGYLSAKDMAALMKIK"
BASE COUNT          344 a          276 c          202 g          336 t
ORIGIN      
        1 gccgcttcaa aagcaacaaa aacacctttg gcttcagcca cgccaaagat ttcattacca
       61 tattgggcta ataaggcttt attagctgtc actacgtgtt tgccattagc aatcgcttgt
      121 aacaccaatt cacgtgctgt agttgtaccg ccaattactt cgaccacaat atcgacttcg
      181 gggtcattga ccaccgcaaa aatatcacgg ctaacagtga caccagttaa atcatattgt
      241 ggataatcac gacgcgcgcc cacatgactg acaataatat cgcggccagt tcgacgacta
      301 atttctgcgg cattcgcttt taataaatta aacgcaccgc caccgactgt acctagcccg
      361 acgatgccaa ttttaactgg tttcacccta tcacctatta tgtaacgaga ttatatattc
      421 aagaataaaa gtcgctaatg atataacgct ttatcaaaaa aatgtagaga gattagacaa
      481 gagatgcgac ttaggcatta aacaatctgc ctaagtcgat tttgtagaat aatttattta
      541 attttcatta atgcagccat atcttttgcc gataaataac cgcccacttg tgtaccatct
      601 tctagaaaca cggctggtgt accacgtaca ccaatttctt ggcctagctc atattgctgt
      661 ttaattgggt tctgacaagt tgcaggagca acaggctgat ttcgcttcat ggccgtcatt
      721 gccgcattac gatctttagc acaccatact gcatccattt tttgcgaagt tggtgatgga
      781 taacccgctc ttggaaaagc caaataacgg atttcgatac ccatttgact taatacagga
      841 atttcttcgt gaatttttcg gcaataacca caatcaacat cggtaaacac ataagcaata
      901 cctttggctt tgccgtttgc ggcagggaaa atcaccatat cttttaaagg cacagctgct
      961 aataattctt tacgatggcc agccatgcct tgctcagtaa tattggttaa tttagcacca
     1021 tttaactcaa taatttcacc ttgtatcata taacgaccgt cagccgtcat ataagccgta
     1081 ccataaccct tggccttaat ttcatatagc cctgccacgc tttgcttaac agaggtaatc
     1141 tctgcatcag gaatagca
//
LOCUS       DAOQMB010000782         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_112554_length_1157_cov_24.509074, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000782 DAOQMB010000000
VERSION     DAOQMB010000782.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112554_length_1157_cov_24.509074"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /gene="repM"
                     /locus_tag="PLY05_13920"
     CDS             complement(<1..679)
                     /gene="repM"
                     /locus_tag="PLY05_13920"
                     /inference="COORDINATES: protein motif:HMM:NF038290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="HNC04436.1"
                     /translation="MIQASYTLGLVEQRLILLAIVGSRSLAETITAETILTVRAEEYA
                     RHFGVELQAGYMALADAVETLFNRRATVEVYDKHRDKMRPMAVRWVTAMQYEENLGCV
                     SLRFGQEVIPLISKLEEQFTSYELQQIAGLKSAYAIRLYELLMQWKASEKTPIFLLQQ
                     FRTQLGLIENEYKMIVDFKKRVLDLAITQINQHTDITVDYEQHKAGRTITGFSFTFSQ
                     KKQAKDVT"
     assembly_gap    852..951
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          292 a          216 c          205 g          344 t
ORIGIN      
        1 gggttacgtc tttagcctgt ttcttttggc tgaacgtgaa cgaaaagcct gtaatagtgc
       61 gccctgcttt gtgttgttcg tagtcaacgg tgatgtctgt gtgttgattg atttgggtaa
      121 tggctaggtc aaggactctt ttcttaaaat caacaatcat cttatattca ttctcaatta
      181 gccctaactg tgttctaaat tgttgcaata gaaagatagg tgttttttcg gatgctttcc
      241 attgcatgag tagttcgtaa agccgtattg cataggcact ttttaagcca gcgatttgtt
      301 gtagctcgta actggtaaat tgctcctcaa gtttgctaat cagagggata acttcttgac
      361 caaaacgaag cgatacgcat cctagatttt cttcatattg catggcggta acccagcgaa
      421 cagccatcgg tcgcatttta tcccggtgtt tgtcataaac ttccacggta gccctacgat
      481 tgaatagggt ttcaacagca tctgctagag ccatatagcc agcttgaagc tcaactccaa
      541 agtgcctagc gtactcctcc gctctcactg tgagaatggt ttcggctgtg atggtttccg
      601 ctaaacttct tgaaccaaca atagcaagaa gaataagcct ctgctccaca agacccaatg
      661 tgtaacttgc ttgaatcaaa cggttatctt tgactattaa atttttcata aaattaaatt
      721 aatgacttgt tgttatttgt cattttactg acatatattt ttttgtcaat gatatgtaag
      781 caaacgtcct tatatgtgta agcaaacgtc cttatatgtg taagcaaacg tccttatatg
      841 tgtaagcaaa cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtccttata
      961 tgccagctta aattattgat ttttaaataa aaatcaaacc ctaaaagtat ttaaaagtat
     1021 ttaaaagtat ttaaaagcgt gtgtgtgcga gtgcgcctgc ctgtgcgcag attaattaaa
     1081 gccgtgtaaa ttacgcaaaa agcaaacgct atgtcgccct aatcgggcgg cttttctccc
     1141 ccctcaatcc ccccagt
//
LOCUS       DAOQMB010000783         1157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_112556_length_1157_cov_13.334846, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000783 DAOQMB010000000
VERSION     DAOQMB010000783.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112556_length_1157_cov_13.334846"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..>1157)
                     /gene="dapE"
                     /locus_tag="PLY05_13925"
     CDS             complement(342..>1157)
                     /gene="dapE"
                     /locus_tag="PLY05_13925"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016143398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HNC04437.1"
                     /translation="AMVVACENFVAQHPNHQGSIAFLITSDEEGVAKDGTVKVVEHLE
                     ARQEKITWCLVGEPSSSKTLGDVIKNGRRGSLNGILKVIGIQGHVAYPQLADNPIHKA
                     APALAELAAEVWDNGNEFFPATSFQISNIHAGTGANNVIPADIEVVFNFRFSTEVTAE
                     QLKARTEAILAKHGLKYDLAWSLSGHPFLTAKGELVAASVEAIKEVAGIDTKLSTSGG
                     TSDGRFIAPTGAQVLELGPINATIHKINECVNVADLDALAKMYEGILVRLLAK"
BASE COUNT          327 a          258 c          241 g          331 t
ORIGIN      
        1 gatggaattg atagacctga tggttggttt tttaggtggt tggcggagag aaataagtga
       61 ggggaactat aagaaatata atgattatga atcttgaagc taattcattt ttgtattggg
      121 tgataaggga gtaggggcaa agacgcgtca tcgtgtttga gattcttctg taggtgggtt
      181 atcccaacgg gcatttataa tgccctctaa acggcattga aagtgaatag ataattgcgc
      241 catggtttgc ccccaattag cgataggtat agtccatcgc acagaggctt gatatgtgta
      301 atgtgggttt cccttcgtca acccacatta caaaccacaa attacttcgc caacaatctc
      361 accaaaatgc cttcatacat cttagccaag gcatctaaat cagcgacatt cacgcattca
      421 ttaattttat gaatcgtggc gttaatcggc cctaattcca acacctgtgc gcctgttgga
      481 gcaataaaac gaccatcgga tgtgccgcca ctggtcgata atttggtatc aatgcctgct
      541 acttctttaa tcgcttcaac actggccgca accaattcac ccttagctgt taaaaacgga
      601 tgaccactca aactccatgc taaatcatat tttaaaccgt gtttggctaa aatggcctca
      661 gtacgcgcct tgagttgttc ggcagtcact tcggtagaaa agcggaagtt aaacactact
      721 tcaatatcgg caggaatcac attgtttgcg cctgttcctg catgaatatt ggaaatttga
      781 aaactggtcg caggaaaaaa ttcattgcca ttgtcccaca cttcagccgc taattcggct
      841 aaggcagggg cggctttatg aatcggatta tcggctaatt gtgggtaagc gacatgacct
      901 tgaatgccaa tcactttaag aatgccattt aatgaaccac gacgaccatt tttaatcaca
      961 tcacccaagg ttttgctgct cgaaggttca cccaccaaac accatgtgat tttttcttgg
     1021 cgtgcttcta aatgttcaac cacttttact gtgccatctt tggctacacc ttcttcatca
     1081 ctggtaatca aaaaggcaat agacccttga tgattcggat gttgggcaac aaaattttcg
     1141 caagcaacca ccatcgc
//
LOCUS       DAOQMB010000784         1156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_112748_length_1156_cov_13.762943, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000784 DAOQMB010000000
VERSION     DAOQMB010000784.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112748_length_1156_cov_13.762943"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PLY05_13930"
     CDS             complement(<1..378)
                     /locus_tag="PLY05_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC04438.1"
                     /translation="MDKFWWVIGLIAVFVGGQWFMMRPNPREQRLMLLREAARKMGFQ
                     PKLVPPPSWYVTDSRQFIACYSLIVPTAKLPYSREQRQKDGSWQVVAGQGLLHKVALI
                     PEEKYLIAIEAQANSISIYWQEDA"
     gene            complement(384..1064)
                     /locus_tag="PLY05_13935"
     CDS             complement(384..1064)
                     /locus_tag="PLY05_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04439.1"
                     /translation="MIIQYFFSFDDGLDLNYDIQVNRRFSIHADYSHAPDWTKLTNNQ
                     CKNCPLDKKVYSHCPAALDLDKVIQDVQKLSALTKVNVRVLTAEREYVKRVTLEEGMR
                     ALMGLIMATSACPVFCDLKPNARTHLPFASKEEAILRLASVYLMKQYFVWREGGIPDW
                     ELKGLIKEHAELQLVNHAFWQRVMAAFKSDANSKALLSFFTVSASISTSLDSQLAKMK
                     SVFFSSIE"
BASE COUNT          370 a          245 c          191 g          350 t
ORIGIN      
        1 tgcatcttct tgccaataaa tactaatact attggcctga gcttctatag caattaaata
       61 tttttcttcg ggaatcaaag caactttgtg taataaacct tgacctgcga cgacttgcca
      121 agaaccatct ttttgacgtt gttcacggct atagggcaac ttggcggtag gtacgattaa
      181 actataacaa gcaataaact gtcgactatc ggtgacatac catgacggtg gtggcactaa
      241 tttaggttga aaccccattt ttcgtgcagc ttcgcgtaat agcattaacc gttgctcacg
      301 cggattgggg cgcatcataa accattgacc acccacaaat accgcaatta aaccaatcac
      361 ccaccaaaat ttatccacaa aatttactca atactgctaa aaaagacact tttcatttta
      421 gctaattgac tgtctaaaga ggtagaaata cttgctgaga cggtaaaaaa acttaataaa
      481 gccttagagt tggcatcact tttaaaagca gccatcacac gttgccaaaa agcatgatta
      541 actaactgta attcggcgtg ctctttgatt aaacctttta actcccaatc agggatacca
      601 ccttcacgcc aaacaaaata ttgtttcatc aaatacaccg atgccaagcg taaaatggct
      661 tcttctttgc tggcaaaggg taaatgagtt ctggcattcg gttttaaatc acaaaagaca
      721 ggacaagcac tggtagccat aattaagccc atcaatgcgc gcattccttc ttctaaagtc
      781 actcgtttaa catattcacg ttcagcggtt aatactcgaa cattcacttt ggtgagtgcc
      841 gatagttttt gcacatcttg aatcacttta tctaaatcta aagccgcagg acaatgacta
      901 tagacttttt tatctaaagg acagttttta cactgattat tcgtcaattt tgtccaatct
      961 ggcgcatggc tataatcggc atgaatcgaa aaacgccgat taacttgaat atcataatta
     1021 aggtctaaac catcatcaaa cgagaaaaag tattgaataa tcatcttgtt gtgatccaat
     1081 tttttaattt ttaaattttc agttattggc cactatagaa cacattcttc atttttacca
     1141 attgactttg gagtga
//
LOCUS       DAOQMB010000785         1155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_112947_length_1155_cov_10.878182, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000785 DAOQMB010000000
VERSION     DAOQMB010000785.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112947_length_1155_cov_10.878182"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLY05_13940"
     CDS             complement(<1..241)
                     /locus_tag="PLY05_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004927117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04440.1"
                     /translation="MQINSTVHTTCAYCGVGCGIKATVENTDKRIVSIQGDTEHPANY
                     GRLCSKGSSLGETISLEGRLLEPQIAGQNVSWPTAI"
     gene            complement(253..>1155)
                     /locus_tag="PLY05_13945"
     CDS             complement(253..>1155)
                     /locus_tag="PLY05_13945"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC04441.1"
                     /translation="FDYDRLLLATGSVPFIIPIANHNHACVLSFRDIRDVNTMLEVTQ
                     HKQRVAVIGGGLLGLEAANGLLKRGVDVTVIHDTPCLMNRQLDSEAAHLLQKQLEANG
                     MKFRMGAMTKEIVALDDGHLSHIAFKNGEDLPTDLVIMAVGIRPNVALAKQIGLQVDK
                     AILVNDTMQTFDPSIYAVGECVQHRGALFGLVAPLYEQAKVCATHLAEIGIGRYVQKA
                     TATTLKVSGVNLFSAGDFSGANAETIVLRDTSSSIYKRLFIKDNKLVGAVLFGDVQDG
                     NWYFDLIKQQQDISDIRADLVFGQ"
BASE COUNT          354 a          280 c          203 g          318 t
ORIGIN      
        1 caattgctgt tggccaagaa acattttgcc ctgcaatttg tggctctaat aaacggcctt
       61 ctaaactaat ggtttcacct aaggatgaac ctttagaaca caaacgacca taattagcag
      121 gatgttcagt atcgccttga atactgacaa tacgtttatc tgtattttct accgtggctt
      181 taatgccaca accgacacca caatacgcac aagtggtatg cactgttgag ttaatttgca
      241 tcacttgcac tcctattgac caaacactaa atcagcacga atatcgctaa tatcctgttg
      301 ctgcttgatt aaatcaaaat accaattacc atcttgcaca tcaccaaata gcactgcacc
      361 gactaactta ttatctttaa taaacaagcg tttataaatg cttgaagagg tatctcttaa
      421 gacaatggtt tcggcatttg cccccgaaaa atcacctgcg gagaataaat tgacaccact
      481 gactttaagc gtggtggcgg tggctttttg tacataacga ccaatgccaa tttcggctaa
      541 atgggtggca cagacttttg cttgttcgta taagggcgca accaaaccaa ataacgcgcc
      601 acgatgttgc acacattcgc ccactgcata aatagatggg tcaaaggttt gcatggtgtc
      661 attgaccaaa atcgctttat cgacttgcaa accaatttgt ttggctaacg ccacattagg
      721 acgaataccc actgccataa tcactaaatc agtcggtaaa tcttcgccat ttttgaaggc
      781 aatatgactt aaatgaccat catctagggc aacgatttct ttagtcatcg cccccattct
      841 aaacttcatg ccattggctt cgagctgctt ttgcaataaa tgggcagctt cgctatctaa
      901 ctgacgattc atcaaacagg gcgtatcgtg aatcaccgtc acatcgacac cgcgttttaa
      961 caaaccatta gccgcttcta gccccaataa accgccacca atcaccgcca cccgttgttt
     1021 atgttgagta acttcgagca tggtgtttac atcacgaata tctcgaaaac ttaacacaca
     1081 cgcatgatta tgattagcaa tgggaataat aaatggcact gaacctgtcg ctaataacaa
     1141 acggtcataa tcaaa
//
LOCUS       DAOQMB010000786         1152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_113573_length_1152_cov_6.140383, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000786 DAOQMB010000000
VERSION     DAOQMB010000786.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113573_length_1152_cov_6.140383"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>1152)
                     /locus_tag="PLY05_13950"
     CDS             complement(74..>1152)
                     /locus_tag="PLY05_13950"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNC04442.1"
                     /translation="DKIYARGDFVRIGYQLLPQAQSSLLRIIPVEKDGRDFARIGLNL
                     HTDFANNSSFGLVTGLRRSWLNRLGAEWQAEGELGETRSLYSELYQPTVLNGEFFIAP
                     WLKIHDEPRDIVTNHETVAQNRVRHTGGGVDIGSVLGKWGEVRLSVSEQRVKWSSSLE
                     NVKTITGESYQQTGYGLKAIFDQLDNPRFPRKGNWAKLDYFHAEQGMGSDKDYQRINF
                     DWRRAFTWQDYTLFATGRGGSSLGTTLPYAEQFQLGGALNLSAYRRSELAGNNYFLTR
                     LLAYKQIKDIPPALGGGVYLGLLAETGAVSMEDKWSSRLFESTPYSVGVVLAADTRLG
                     PFYLTIAKGDQDRHTANLTLGVTY"
BASE COUNT          353 a          272 c          198 g          329 t
ORIGIN      
        1 ttacttacaa ggcgaatgct cctaaataaa ctataaacct cataattaag cccctgttga
       61 ggggcttaat tatttaatag gtcacaccta aggttaaatt agccgtatgt cggtcttgat
      121 cgcctttggc aatggttaaa taaaatggcc ctaaacgggt atctgcagct aacaccaccc
      181 ctacactata aggtgtactt tcaaacaaac gcgatgacca cttatcttcc atagacacag
      241 cacctgtttc tgctaacaaa cctaaataca caccgccccc caatgctggt ggaatgtctt
      301 taatttgttt ataagccaat aatcgtgtta gaaaataatt attacctgct aattccgaac
      361 gccgataagc cgataaattt aacgcaccac ctaattgaaa ttgttcagca taaggtaaag
      421 ttgtacctaa agagctacca cctcggcctg tcgcaaataa ggtatagtct tgccatgtaa
      481 aggcccgtcg ccaatcaaaa ttaatgcgtt gataatcttt atcactaccc ataccctgtt
      541 ctgcatgaaa ataatctaat tttgcccaat tgcctttgcg tggaaaacga ggattgtcta
      601 gttggtcaaa aatggctttt aggccataac ctgtttgctg ataactttct cctgtaatag
      661 ttttaacgtt ttctaaagac gatgaccatt tcactcgttg ttctgaaaca ctcaaccgaa
      721 cctcgcccca tttccctaac accgagccaa tatcgacacc gccacctgta tgacgtactc
      781 ggttttgtgc aacagtttcg tgattggtaa caatatcgcg tggttcatca tggattttta
      841 gccaaggggc aataaaaaat tcaccattta ataccgttgg ttgatatagc tcgctatata
      901 aagagcgtgt ttctcctaac tctccttcgg cttgccactc cgcccctaaa cgatttaacc
      961 aactacggcg taagcctgtc actaaaccaa aggaagagtt attagcaaaa tcagtatgca
     1021 agtttaaacc aatacgcgca aagtctcggc catctttttc gacaggaata atccttaaca
     1081 aggagctttg agcttgtggt aataattgat agccaatacg gacaaaatca ccacgcgcat
     1141 aaattttatc aa
//
LOCUS       DAOQMB010000787         1151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_113767_length_1151_cov_9.523723, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000787 DAOQMB010000000
VERSION     DAOQMB010000787.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113767_length_1151_cov_9.523723"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLY05_13955"
     CDS             complement(<1..421)
                     /locus_tag="PLY05_13955"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HNC04443.1"
                     /translation="MPLIRLVYASQFASPFNEQDAQDIIEVSMRNNRKHDITGVLCYS
                     NYYYLQCLEGGRDEVNHLYQKIAMDKRHVRPVILMYQDILYRQFDDWYMGHIPAINIS
                     GGMVLKYSRERQFNPYSMTPQSAYEFLVELSKVNAVLE"
     gene            complement(556..>1151)
                     /locus_tag="PLY05_13960"
     CDS             complement(556..>1151)
                     /locus_tag="PLY05_13960"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HNC04444.1"
                     /translation="NDVVLAICAGALRRFLQEQETLPNEALTVMLPVNIRPKDDPGGG
                     NAVGAILASLATDIADPLTRVQTIIQSTTKAKEQLQGMSKSAIMQFSSMLMAPMLLSQ
                     IPGAAGRVRPAFNLAISNVPGPDKTLYFRGYRLDSYYPVSIPFLGYGLNITVVTYVDT
                     LNFGFIGCRDTVPHLQRLAVYSRDALEELEQIFGLAS"
BASE COUNT          368 a          254 c          216 g          313 t
ORIGIN      
        1 actccaacac tgcattgact ttcgataact ccaccaaaaa ctcgtaggcg gattgagggg
       61 tcatgctata aggattaaat tggcgttcgc gcgaatactt tagcaccatg ccaccactaa
      121 tgttaattgc aggaatatgc cccatatacc agtcatcaaa ttggcgatat aaaatgtctt
      181 gatacatcaa aataacaggc cgtacatgac gtttatccat agcgattttt tgatataagt
      241 gattaacttc gtctcgacca ccttccaagc actgtaaata gtaataattg ctatagcaca
      301 atacgcctgt aatatcgtgt ttacgattat ttctcattga tacctcaata atatcttggg
      361 catcttgctc attaaaaggc gaagcaaatt ggctggcata aaccaaacga attagtggca
      421 tataatgaca ctctatatta aaaacgaata atctcagttt agcgttaata taaagaattg
      481 ttgattgaac aaaactaggt cgttattaat tgttgtgtac tgacaccgta ttttggtcag
      541 tacacataaa taaacttaac tcgctaagcc aaaaatttgt tctaactcct ccaaagcatc
      601 acgactatac accgctaaac gttgcagatg cggcacagta tctcgacaac caataaaacc
      661 aaagtttagc gtatcgacat aagtgaccac ggtaatattc aggccatacc ctaaaaatgg
      721 aattgacaca ggataataag aatccaaacg atagcctcta aaatacaagg ttttatcagg
      781 gccgggtaca ttagaaatcg ctaggttaaa ggctggacgc actcgccctg ccgcacctgg
      841 gatttgtgac aacaacatcg gtgccattaa catactgcta aattgcataa tcgctgattt
      901 agacatccct tgtaactgtt ctttggcttt ggtggtagat tgaataatgg tctgtacgcg
      961 cgttaacggg tcagcaatat cggttgccaa cgaggccaaa attgcgccta ccgcattgcc
     1021 gccaccaggg tcatctttgg gacgaatgtt gacaggcaac atcaccgtta atgcttcatt
     1081 aggcaaagtc tcttgttctt gcaaaaagcg acgtaacgcc cctgcacaaa tggctaacac
     1141 cacatcattg a
//
LOCUS       DAOQMB010000788         1148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_114381_length_1148_cov_5.815188, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000788 DAOQMB010000000
VERSION     DAOQMB010000788.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114381_length_1148_cov_5.815188"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLY05_13965"
     CDS             <1..426
                     /locus_tag="PLY05_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04445.1"
                     /translation="AELLNVQILGVLAGQGSWSDYQLTASLPKQISHFSLKLLEWTGH
                     GYITRDYLNFVVSVAAEVEAETVTVEELTESPQEKATPVTPSLNDATEADLTAIKGVS
                     KSLAQVIKKSQPYSAWQQVAKLKGVGGKVLAKLQAVFVI"
     gene            530..>1148
                     /locus_tag="PLY05_13970"
     CDS             530..>1148
                     /locus_tag="PLY05_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC04446.1"
                     /translation="MKKRTSIIVSALCISACQQPMIVANKPPAMYQSSISSDGTSVVV
                     KLADGQQRVVSSGLPSYSAQIGQVTVQRGERSFTIQSTKPTLSTEGKVTGSVFKPNAP
                     EGAHWQLLKLNGQDIDSKKSKRPPYLRMIGGQMHGLTGCNVMSGIYHRDEEKMQFMRV
                     QGTTSNCDYQELERNFIGALQRLRLWRVTPDYHLQFLDEKNQVVAE"
BASE COUNT          352 a          200 c          276 g          320 t
ORIGIN      
        1 gctgaattgc ttaatgtgca gattttaggg gtcttagcag gacaaggttc atggagtgac
       61 tatcaactca cagcgtctct ccccaagcaa atctcacatt tctcactcaa attgttggaa
      121 tggacaggcc atggttatat cacgcgggat tatttgaatt ttgttgtgtc tgttgctgct
      181 gaggtagaag ctgaaactgt cactgtcgaa gagttgacag aatcgcccca agaaaaggct
      241 acacctgtta ctccttcttt aaatgacgca acagaagcag atttaacagc gatcaaaggc
      301 gtctctaaaa gtcttgccca agtaattaag aaaagtcagc cgtatagtgc ttggcagcaa
      361 gttgctaagt taaaaggggt gggtggaaaa gtcttggcaa aattacaagc tgtatttgtg
      421 atttgaaata gttagtttta atcggtatgc tcttgcttta aagcaataac tatataagtc
      481 ctaatgttga ttaggttgtc tctgttttaa agcaaagagt atggacttta tgaaaaaaag
      541 aacgagtatc atcgtcagtg ctttatgtat aagtgcatgt caacaaccca tgattgtagc
      601 caataagcca ccagctatgt atcaaagtag tattagtagc gatggtacat cggtcgttgt
      661 caaattggct gatggtcaac agcgagtggt aagctctggt ttgccaagtt attctgcaca
      721 aataggccaa gtgacggttc aacgtggtga acgcagtttt accatccaat cgacaaagcc
      781 taccctaagt acagaaggaa aagtcacagg tagtgtattt aaaccaaatg ctccagaggg
      841 ggcacattgg caattactga agttgaatgg tcaagacatt gacagcaaaa aaagtaaacg
      901 ccccccttat ttgcgtatga ttggtggaca aatgcatggg ctgacaggct gtaatgtgat
      961 gagtggcatt tatcatcgtg acgaggaaaa aatgcaattt atgcgcgtgc aagggacgac
     1021 aagcaattgt gactatcaag aattagagcg gaattttatt ggtgcgttac agcgtttgcg
     1081 attgtggcga gtaacacctg attatcattt gcagttttta gatgaaaaaa atcaggtggt
     1141 ggcggaat
//
LOCUS       DAOQMB010000789         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_114765_length_1146_cov_10.429881, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000789 DAOQMB010000000
VERSION     DAOQMB010000789.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114765_length_1146_cov_10.429881"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            276..>1146
                     /locus_tag="PLY05_13975"
     CDS             276..>1146
                     /locus_tag="PLY05_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04447.1"
                     /translation="MSSKALQVVRGKTALITGASSGIGLTVAKKLGKAGAHVLLVARG
                     VEKLEQTAEMIRKVGGTATIYPCDLSDLKAIDAMAEQILADHGHVDIVVNNAGRSIRR
                     AVIESLDRFHDFERTMQLNYFGAVRLILRLLPSMITNKKGQIINISSIGCLANSPRFS
                     AYVASKAALDAFTRCLSAEVKQYNIQTTAIYMPLVRTPMIAPTKMYDYVPTYTPDQAA
                     NLVIRAIVDKPKRIKTPLGQTAELSYALWPKVNDAILNLGFKLFPSSAAAKGIKEEKP
                     SAAAIVMANVLQGQ"
BASE COUNT          345 a          235 c          244 g          322 t
ORIGIN      
        1 actttgccaa aaaattggat ttacccaata tcaaaatgcc gagcagcgcc aaaaacttat
       61 ttaacaaatt tgctgaaagt gagttagccg ttaccatttc taaagcgatt ggtgcgccaa
      121 tttctaccct cacctatgcc tttaataaat ttgagtttga tgacaaaaat actcgccaag
      181 ccttaaaagg tagtggcata agctgtccac gtctcgaaga atatgctgat aaactgtggg
      241 attattggga atggaactta gacagtgctg cgtttatgtc gagcaaagcc ttacaagtgg
      301 tgagaggcaa aaccgcttta attacaggtg catcgagtgg cattggttta acggttgcta
      361 aaaaattagg caaagcaggc gcgcacgttt tgttagtggc gcgcggtgta gaaaaacttg
      421 aacaaactgc cgaaatgatt cgtaaagttg gcggcacagc cactatttac ccgtgtgatc
      481 tgtccgattt aaaagccatt gatgcgatgg ccgaacaaat tttagcagat cacggtcatg
      541 ttgatattgt ggtaaataat gcaggacgtt ccattagacg tgcggtgatt gaatcgcttg
      601 accgtttcca cgactttgaa cgtaccatgc aattaaatta ttttggcgca gtgcgtttaa
      661 ttttgcgttt attaccatcc atgataacca ataaaaaagg tcaaattatc aatatttcgt
      721 ctattggttg tttggctaat tcaccacgct tctcggctta tgtggccagt aaagcagcgt
      781 tggatgcgtt tactcgctgt ttatctgccg aagtaaaaca gtacaacatt caaaccaccg
      841 ccatttatat gcctttagtg cgtacaccga tgattgcacc aactaaaatg tatgattatg
      901 tacccacata cacgcctgac caagccgcaa acttggtgat tagagcgatt gtggataaac
      961 ctaaacggat taaaacacca ctcggacaaa cagctgagtt gtcttatgcc ttatggccaa
     1021 aagtcaatga tgcaatttta aacttaggtt ttaaattgtt cccatcatct gctgcggcta
     1081 aaggcattaa ggaagaaaaa ccatcagccg ccgcgattgt catggccaat gtgttacaag
     1141 gtcaac
//
LOCUS       DAOQMB010000790         1146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_114774_length_1146_cov_6.526123, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000790 DAOQMB010000000
VERSION     DAOQMB010000790.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114774_length_1146_cov_6.526123"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="PLY05_13980"
     CDS             complement(<1..871)
                     /locus_tag="PLY05_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005078512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04448.1"
                     /translation="MASKTLSVVRGKTVLITGASSGIGLTVAKKLGKAGAHVLLVARG
                     VDKLEQTAEMIRKVGGTATIYPCDLTNLEAIDQLVAQVLADQGHVDILINNAGRSIRR
                     AVVESFDRFHDFERTMQLNYFGAIRLIMGLLPSMQAAKRGQIINISSIGCLANAPRFS
                     AYVASKAALDAFTRCLSAEVKQYNIETTAIYMPLVRTPMIAPTKMYDYVPTYTPDQAA
                     NLIIKAIVSKPKRIKTPLGQTAELSYALWPKINDAILSLGFKLFPSSAAARGVKEEKP
                     SAAAIVMANVLQGQ"
BASE COUNT          343 a          252 c          226 g          325 t
ORIGIN      
        1 gttgaccttg taacacattg gccatgacaa tcgcggcggc tgatggtttt tcttccttca
       61 caccacgagc agcagcagac gatgggaaca acttaaagcc taagcttaaa atcgcatcat
      121 tgatttttgg ccataaagca taagacaact cagcggtttg acccaacggt gttttaatcc
      181 gttttggttt gctcacaatg gctttaataa tcaaattagc tgcttggtct ggggtgtatg
      241 tgggtacata gtcatacatc ttagttggtg caatcatcgg tgtacgcacc agcggcatat
      301 aaatcgcagt ggtttcgatg ttgtactgtt ttacttcagc agataaacaa cgagtaaacg
      361 catccaacgc cgctttactg gccacataag ccgagaagcg aggggcgttt gccaaacaac
      421 caattgacga aatattgata atttgaccac gtttggccgc ttgcattgat ggtaataagc
      481 ccataatcaa acgaattgca ccaaagtaat ttaattgcat agtacgttca aaatcgtgga
      541 aacgatcaaa cgattcaacc actgcacgac gaatcgaacg acctgcatta ttaattaaaa
      601 tatcaacatg accttgatcg gctaatactt gcgccactaa ttggtcaatc gcttctaaat
      661 tggttaagtc acaaggataa atcgttgctg tgccacccac tttacgaatc atttccgcag
      721 tttgttcgag tttgtccaca ccacgtgcga ctaataaaac atgtgcacct gctttgccca
      781 attttttggc cacggttaaa ccaataccac tagaagcacc agtaattaac acggttttac
      841 cacgaaccac acttaaagtt ttgctggcaa taaaggacgt agcatctaaa ttccactccc
      901 agtaatccca caatttatca gcataagttt ctaacgcagg gcactcaata ccactgcctt
      961 ttaaagcagt acgagtattt ttgtcatcaa attcaaattt gttgaacgca taggtgaggg
     1021 tagaaatggg cgcaccaatg gctttagaaa tggtaacggc taactcactt tcagcaaatt
     1081 tgttaaataa atttttggcg ctgctcggca ttttgatatt gggtaaatcc aattttttgg
     1141 caaagt
//
LOCUS       DAOQMB010000791         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_114951_length_1145_cov_27.157798, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000791 DAOQMB010000000
VERSION     DAOQMB010000791.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114951_length_1145_cov_27.157798"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="PLY05_13985"
     CDS             <1..1095
                     /locus_tag="PLY05_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04449.1"
                     /translation="ERVVSSAAKALGTGRPDYNETVEGFIQSAKAKSAPYYAQLNGYQ
                     YPIDDELRTIIKRASPFFARSNLAATVDDLGGATLTDALDPSTKFISFDQLKLLRQSL
                     QSSEDNYMTSLTNKDKDLGVRVSRLKNELTRKLDELSPKTPLGDSVHALASGAYAGEM
                     QLKNAVEQGRLIFREDAMNIRDSLKNMTQSEKDAFRLGVYQAIVDKAGRQSGRTELMN
                     IYKNPAIADRLKAVFGNDYRKFASKLAAEFELKKFQRVAGGSQTAGRQQGLNDLELSP
                     LRDASEGVGALASGSVRGSVSPLTRLWQGVSVPESVRTDMAKTLLLEGAPAQAKLQSL
                     NELVKYINQQKQINAMRFGSTFGQLQGGNQ"
BASE COUNT          370 a          206 c          260 g          309 t
ORIGIN      
        1 gagcgtgttg taagtagtgc agctaaggca ttaggtactg gtcgtcctga ttataatgaa
       61 acagtagagg gttttataca gtctgctaaa gctaaatcag caccttatta cgcacagtta
      121 aacggttatc aatacccgat tgatgatgaa ttaagaacca ttattaaaag agcgagtccg
      181 ttttttgcta ggtcaaattt ggcagcaacc gttgatgatt tagggggagc aacattaaca
      241 gacgcattag accctagtac aaaattcatt agttttgacc aattaaaact attgagacaa
      301 tccttgcaat caagcgagga taattacatg acaagcctta caaataaaga taaagattta
      361 ggcgtgcgtg tttctcgttt aaaaaacgaa ctaacgcgaa aactagacga attatcccca
      421 aaaacaccac taggcgattc ggttcacgct ttagcaagtg gtgcttatgc gggtgaaatg
      481 cagcttaaaa acgcagtcga gcaaggtcgg ttgatatttc gtgaagatgc gatgaatata
      541 cgcgattcac ttaaaaacat gacgcaatca gaaaaagacg cctttcgttt aggggtttat
      601 caagcaatcg ttgacaaagc gggacgacaa agcggacgta ctgagttaat gaatatttac
      661 aaaaaccctg ctattgctga ccgtttaaaa gctgtttttg gtaacgatta tcgaaaattt
      721 gcatcaaaat tagcggctga gtttgagtta aagaaatttc agcgtgtggc aggtggtagc
      781 caaaccgcag gacgacaaca aggcttaaat gacttagagc ttagcccatt gcgtgatgca
      841 agcgagggcg taggggcttt agcaagtggt agcgtgaggg gttcagttag cccattaaca
      901 agattatggc aaggtgtatc tgttcctgaa tctgtgcgta cagatatggc caaaacattg
      961 ttattagagg gtgcgcctgc acaagcaaaa ctgcaaagtt taaatgagtt agtaaaatac
     1021 attaatcagc agaaacagat caatgcgatg cgttttggtt caacatttgg ccaacttcaa
     1081 gggggcaatc aataatgtct tattcacttt caccactttt aaaacctcgc ttttttgtta
     1141 atgcg
//
LOCUS       DAOQMB010000792         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_114959_length_1145_cov_6.633028, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000792 DAOQMB010000000
VERSION     DAOQMB010000792.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114959_length_1145_cov_6.633028"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="PLY05_13990"
     CDS             <1..719
                     /locus_tag="PLY05_13990"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNC04450.1"
                     /translation="QSNQDKVLKLLRFASTLSGDAVESVALADYVARMKEGQEKIYYL
                     TAESFTAASQSPHLEVFRKKGVEVLLLTDRLDEWLMTHLTEFDGKQFQHIAKGDLDLG
                     SLSSEEDKKAEEQLQETSQPLVERLQKILADKVQAVKATQRLTDSPACLVRSQYDLGA
                     AMRQLLEASGQKLPESKPTLEINPSHPLVARLSNTNDETRFETLALIVLDQATLAEGS
                     ALADPAAYVKRLNSLLMELV"
     gene            821..>1145
                     /locus_tag="PLY05_13995"
     CDS             821..>1145
                     /locus_tag="PLY05_13995"
                     /inference="COORDINATES: protein motif:HMM:NF015747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HNC04451.1"
                     /translation="MILSKPRVAVLGGGSFGTVIANLAAGNGCQTTQWLRDVELVAEM
                     QTTHQNTRYLKGFDLHPELQFSTDLAQTVSQADLVFVAIPSKAFRQVIQTAKPHFQAK
                     QMLVSM"
BASE COUNT          355 a          220 c          253 g          317 t
ORIGIN      
        1 atcaaagtaa ccaagataaa gtcttaaaat tattaagatt tgcctctacc ttatcaggcg
       61 atgcagttga gtctgtggct ttagctgatt atgtcgcgcg tatgaaagaa ggccaagaga
      121 aaatttatta tctcactgcc gagagcttta ctgccgctag ccaaagccct catttagaag
      181 tgttccgcaa aaaaggcgtg gaagtcttgt tgttgactga ccgtttagac gaatggttaa
      241 tgacccattt aaccgagttt gatggcaaac aattccaaca tattgccaaa ggtgacttag
      301 acttaggcag tttaagcagc gaagaggaca aaaaagccga agaacaattg caagagacca
      361 gtcaaccttt agttgaacgt ttgcaaaaaa tcttagctga taaggttcaa gcggtaaaag
      421 ccactcaacg cttaacggat tcgccagcgt gtttggtgcg tagccaatat gatttaggtg
      481 cagccatgcg ccaattatta gaggccagtg gtcaaaagtt accagaatct aaacctactt
      541 tagagattaa cccaagccat cccttagtag cacgactcag taacacgaat gacgaaacac
      601 gttttgaaac cttagccctg attgtcttag accaagccac cttagccgaa ggcagtgcgc
      661 ttgccgatcc agcggcttat gtgaagcgtt taaatagctt attgatggaa ttagtgtaag
      721 attttcatca attgataaag ggcgcataat gcgcccttta ttttgctcaa acgtctaaag
      781 tttgctatcg tgaagagaaa taatttacat aggaataagt atgattttat ctaagccgcg
      841 tgttgcagta ttagggggcg gaagttttgg tacggtaatt gcgaatttgg cagcaggtaa
      901 tggttgtcaa accacgcaat ggttacgtga tgttgaatta gttgctgaaa tgcaaaccac
      961 acatcaaaat acccgttatc tgaaaggttt tgatttgcat ccagagttac aattcagtac
     1021 cgatttggca caaacggtta gtcaagctga cttagtgttt gttgctattc ctagtaaagc
     1081 ttttagacag gtgattcaaa cggcaaaacc gcattttcaa gctaaacaaa tgcttgtcag
     1141 tatga
//
LOCUS       DAOQMB010000793         1145 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_115007_length_1145_cov_3.378899, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000793 DAOQMB010000000
VERSION     DAOQMB010000793.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115007_length_1145_cov_3.378899"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..974)
                     /locus_tag="PLY05_14000"
     CDS             complement(123..974)
                     /locus_tag="PLY05_14000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004822713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNC04452.1"
                     /translation="MHYPIPNPLLPIRRDVRFNLPADQISHWHYTGGSIFTAFLNTFS
                     IVLPVGERFFIDSVRAYRDQITDPELKKEITAFIGQEAMHGREHEEYNAALFSQVPIA
                     PKFEKLVSGILKGLTKHAPKAFCLSGTIALEHFTALLGDSVLSEPLVSEGAEPHYAAL
                     WRWHALEETEHKGVAFDVWDKVMGRGVKAYSLRAFGLVLATVIFWGLVIPVFIQVLRT
                     EGKLTDWDGWRKFYRFTMGDIGLLRIQLANYMDYFRPNFHPWDHDNRQYLAQIDDFLA
                     AQQKLAA"
BASE COUNT          364 a          230 c          233 g          318 t
ORIGIN      
        1 gctttctgcc aatcgttaga caggctcacc aagaatgaga gaatttacta tggttttcaa
       61 aatagacgcg gtttagctcg cattttatga cggtttatac gagctacaaa aataactgct
      121 atttaagcgg ccaatttttg ttgtgcggct aaaaagtcat caatttgtgc taaatattga
      181 cgattatcat gatcccaagg gtgaaagttt ggacggaaat aatccatata gttagcgagt
      241 tgaatacgta acaagccaat atcacccatc gtaaaacgat aaaacttacg ccagccatcc
      301 caatcggtca atttgccttc ggtacgtaat acctgaataa acacaggaat caccaagccc
      361 caaaaaataa ctgtcgctaa aactaagcca aaggcacgta agctgtaggc tttgacaccg
      421 cgtcccataa ctttatccca aacatcaaaa gcgacacctt tatgttcggt ttcttcaagg
      481 gcatgccaac gccataacgc agcataatgc ggttctgccc cctcagaaac taaaggttca
      541 cttaaaacgc tatcgcctaa aagcgcggta aaatgctcaa gagcgatagt gccacttaaa
      601 caaaaggctt taggcgcatg tttggttaaa ccttttaaaa tacccgacac taatttttca
      661 aatttagggg ctattggtac ttggctaaat aaagctgcat tatattcttc atgctcacga
      721 ccgtgcatgg cttcttggcc aataaacgct gtaatctctt ttttgagttc gggatcagta
      781 atttggtcgc ggtaggcacg gacactgtca ataaaaaaac gttcaccgac aggtaaaaca
      841 atggaaaatg tattcaaaaa ggcggtaaaa attgatccac ctgtataatg ccagtgacta
      901 atttgatcgg caggtaaatt aaaacgcaca tcgcggcgaa taggtaacaa aggattagga
      961 ataggatagt gcatagagac gctcgttatg gcttagtcgt ttttggaata atgaccaaag
     1021 cactaggaga tatttgcttc gatcagggcg tggttatgat ttccctatga gttgattgta
     1081 gaagccaata ttaattttgt acagtcgtac aacaagttgt ttttgggttc ttaattttgt
     1141 attgc
//
LOCUS       DAOQMB010000794         1142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_115645_length_1142_cov_3.678013, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000794 DAOQMB010000000
VERSION     DAOQMB010000794.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115645_length_1142_cov_3.678013"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..565)
                     /locus_tag="PLY05_14005"
     CDS             complement(113..565)
                     /locus_tag="PLY05_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005001971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNC04453.1"
                     /translation="MNTYHFTIVVRDAYVDMPDIEDSFFEAGCDDALLCSYNDTVYLE
                     FDRQADSAEQAITSALENIRSIGFQQLIVQEGGVSTLSEMAERAGLTRAALSQYAKSK
                     RGCGDFPKPVYGVASGSALYSWAEVAEWLYKQGKLAQTHYDVARVALR"
     gene            complement(569..802)
                     /locus_tag="PLY05_14010"
     CDS             complement(569..802)
                     /locus_tag="PLY05_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04454.1"
                     /translation="MKKHPNKHIREAIKHAMANGWEIVDAGKSAHAFCRLRCVAGHTE
                     HQMSIWSTPKSPENHAKQILHKVQQCRDEQQEQ"
     gene            complement(889..>1142)
                     /locus_tag="PLY05_14015"
     CDS             complement(889..>1142)
                     /locus_tag="PLY05_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04455.1"
                     /translation="YLRSVAPKELNTSHLYKGAVPFIALQLVMVAVLMAYPDLVSHDS
                     KEQTTFSNSQDLSNVGGQAASADEAIRLMQEIAKEFRDK"
BASE COUNT          317 a          258 c          214 g          353 t
ORIGIN      
        1 cgcagtggct gcgcctaaag cagcaatttt ttgtaaggct tgacgacgtt gcatggttca
       61 ttctcatttc tttaggactc ataacaagtc caccacagtg tttgtgacag ttttatctta
      121 aagcgacacg cgcaacgtca taatgcgttt gcgctaattt accttgtttg tataaccatt
      181 ctgccacttc tgcccatgaa tacaaggctg agcctgaagc aactccgtac acaggtttag
      241 ggaagtcacc acaaccacgt ttacttttgg catattgaga caaggcagca cgcgttaaac
      301 ctgctcgttc tgccatttcg gataatgtag atacaccgcc ttcttgtaca atcagttgtt
      361 gaaagccgat tgagcgaatg ttttctaaag ccgatgtaat cgcttgctcg gcactatcgg
      421 cttgacggtc aaactctaaa tagacggtat cattgtaact acataataaa gcatcatcac
      481 agcctgcttc aaaaaagcta tcttctatat caggcatatc gacataagca tcacgcacca
      541 caatagtaaa atgataagta ttcattattt attgctcctg ttgttcatca cgacactgct
      601 gcactttatg caaaatctgt ttggcatgat tttcgggact tttaggtgta ctccaaatac
      661 tcatttgatg ttctgtatgc ccagcaacac accgtaaccg acaaaaggca tgcgccgatt
      721 tgcctgcgtc cactatttcc caaccattag ccatagcatg tttaatcgct tctctaatgt
      781 gtttattggg gtgctttttc atcttaaaga ccttaaatat agtataaaga ggtgtttaca
      841 aatgtaaaca cctctttata tcagtttgag gaattatgcg gttattgctt atttatctct
      901 aaattctttg gcaatttctt gcattaaacg aatcgcttca tcggcactgg cggcttggcc
      961 accgacattc gacaaatctt ggctattgct aaaggttgtt tgctctttag agtcatgact
     1021 aactaaatct ggataggcca tcaacaccgc caccatgact aactgtaagg caataaatgg
     1081 cacagcccct ttgtataaat gactggtatt aagctctttg ggtgcaacac tgcgtaaata
     1141 aa
//
LOCUS       DAOQMB010000795         1140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116025_length_1140_cov_6.691244, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000795 DAOQMB010000000
VERSION     DAOQMB010000795.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116025_length_1140_cov_6.691244"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="PLY05_14020"
     CDS             complement(<1..263)
                     /locus_tag="PLY05_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04456.1"
                     /translation="MIIEYFFDFDDEKQLHFQVNLDRQFDFKQDLSNAPEWTKLNNNK
                     CSNCPLNSNEFSHCPAALDLDKVIREFQQIAAITKLRVTVITP"
     gene            complement(430..>1140)
                     /locus_tag="PLY05_14025"
     CDS             complement(430..>1140)
                     /locus_tag="PLY05_14025"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC04457.1"
                     /translation="RRWLLVDEQGKFVTQRQYAQMALLKVHVDNNVLLVNAPNMPTLI
                     ATPNYPHEISVSIWQDTVMALTVCEQADVWFSQFLGVNVRLVFFPDYSQRVVDTAWAG
                     EGHQTAFSDGFPILVISQASFDDLSQRWSKTVDWRRFRPNIIVAGDFAPYSEDTWQTL
                     KIGELELDLVKPCSRCVIPSIDPETTVKDSSINKVLAEHRRQADGKVYLGQNAIIRHL
                     PEGFVLAQGQQVEIISKN"
BASE COUNT          360 a          241 c          202 g          337 t
ORIGIN      
        1 tctggcgtaa ttaccgtaac gcgcagctta gtaatagcag caatttgttg aaattctcga
       61 atcactttat ccaaatcaag cgccgcagga caatgactaa actcattact gtttaacggg
      121 caattagaac atttattatt atttagttta gtccactcag gggcattact taaatcttgt
      181 ttaaaatcaa attgacggtc taaatttact tgaaaatgta attgtttttc atcgtcgaaa
      241 tcaaaaaaat actcaataat catcacaggg aactctgtta gtctttatag gtttttactc
      301 tttttagttc cattagacta aaatattcat cgcatttacg caagattatc gtacacagac
      361 ttaagcatag gattgtagtc aatgcgtaag tctggattag gggcggttat ggtgacgaaa
      421 caagccattt taatttttgc tgataatttc tacctgttgt ccttgtgcta aaacaaatcc
      481 ttcaggcaaa tgacgaataa tcgcattttg accaagataa actttaccat ccgcttgacg
      541 acgatgttcg gctaaaacct tgttaataga gctatctttt acggtcgttt cagggtcaat
      601 acttggaatc acacaacgtg aacaaggttt gactaagtct aattccaact caccaatttt
      661 cagcgtttgc catgtatcct cactataagg agcaaagtca cctgcaacaa taatattggg
      721 tcgaaaacgt cgccaatcaa cggttttaga ccaacgttga ctcaaatcat caaaactggc
      781 ttggctaatc acaaggatgg ggaaaccatc ggaaaaagca gtttgatgcc cttctcctgc
      841 ccatgccgta tcaacaacac gctggctata atcaggaaaa aagactaggc gcacattaac
      901 gcccaaaaac tggctaaacc aaacatcagc ttgttcacac accgttaaag ccatcaccgt
      961 atcctgccaa atagacaccg atatttcgtg tggataatta ggtgttgcga ttaatgttgg
     1021 catattggga gcattgacga gtaacacatt attatcaaca tgaaccttta ataaagccat
     1081 ttgggcatat tgccgttgag tgacaaactt tccttgttca tccaccaaca gccatcgtct
//
LOCUS       DAOQMB010000796         1139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116248_length_1139_cov_6.429889, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000796 DAOQMB010000000
VERSION     DAOQMB010000796.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116248_length_1139_cov_6.429889"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PLY05_14030"
     CDS             <1..338
                     /locus_tag="PLY05_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04458.1"
                     /translation="RDEKKLINLLADNAQIKLTLSKMGQTFSLSKPQYLQQIKAAWHF
                     ADNDKYQVSDLKYSLTQAGLLATVNLKLNESRTMFAQNLNIEQNMEIKLMLIDDKAQI
                     SAIKSTSNF"
     gene            340..1005
                     /locus_tag="PLY05_14035"
     CDS             340..1005
                     /locus_tag="PLY05_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HNC04459.1"
                     /translation="MNTELLPHIELNPPTPATATVIWLHGLGASGDDFVPIIPELDLP
                     KELAVRFIFPHAPQIPVTINGGYIMPAWYDITAMSIEREIDQQQLFASSAAIAQFIDR
                     EIARGIAGERIILAGFSQGGAVAYQTALTYPKSLGGLLVLSSYFATHKTITINPANQQ
                     LSIAIFHGIYDSVVNELLGSQALQNLRTMGYQPYYRSYEMEHEVCLEEVMDISNWLQA
                     RLK"
BASE COUNT          340 a          235 c          239 g          325 t
ORIGIN      
        1 agcgtgacga aaaaaagctg attaatttat tggctgataa tgctcaaatc aaactgactt
       61 taagcaaaat ggggcaaacg tttagtttgt ctaaacctca atatttgcaa caaatcaaag
      121 cagcttggca ttttgccgat aatgacaaat atcaagtcag tgaccttaaa tatagtttga
      181 cacaagctgg cttattggca accgtcaatt taaaactcaa tgaaagccgc acgatgtttg
      241 ctcaaaacct taatattgaa caaaacatgg aaattaaatt aatgttaatt gatgataagg
      301 cacaaatttc agcgattaaa agcacgagta atttctaaga tgaacacgga attattgcca
      361 catatcgaac ttaatccacc tacacctgcc accgcaacgg tgatttggtt acatggttta
      421 ggtgcaagtg gtgatgattt tgtgccgatt attcccgagc ttgacttacc caaagagtta
      481 gcggttcgtt ttatttttcc tcatgcgcca caaatccctg tcaccatcaa tggcggttat
      541 attatgcctg catggtatga tattactgcc atgagtattg agcgcgaaat tgaccagcaa
      601 cagctttttg cctcaagtgc ggctattgct cagtttattg accgtgaaat tgcgcgcggc
      661 attgcgggtg agcggattat tttggctggt ttttcgcaag gcggtgctgt ggcctatcaa
      721 acagcattaa cttaccctaa atcgttgggt ggattattgg tgttatcgag ctattttgcg
      781 acccataaaa ccatcactat taaccctgcc aatcaacaac tgtcaattgc gatttttcat
      841 ggtatttatg acagcgtggt caatgagtta ttaggcagcc aagccttaca aaacctacgc
      901 acgatgggtt atcaacccta ttaccgcagt tatgaaatgg aacatgaagt gtgtttagaa
      961 gaagtgatgg atatttcgaa ttggttgcaa gcaaggctta aataactata ccgtacaagg
     1021 tcactgagcg gagtcgaagt gctaattcgt ggctttgact ccgctcagcc aactcatgca
     1081 aggttaaaac aagcgcgcaa tcaacgccgt caccgccgtt tctacgcgta aaatccgcg
//
LOCUS       DAOQMB010000797         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116444_length_1138_cov_20.763620, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000797 DAOQMB010000000
VERSION     DAOQMB010000797.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116444_length_1138_cov_20.763620"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="PLY05_14040"
     CDS             <1..1134
                     /locus_tag="PLY05_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HNC04460.1"
                     /translation="AFEKGSGVWLYDSDGNAYLDALTGIAVCGLGHAHPVVAEAIANQ
                     AATLVHTSNLYQIPLQEALGQALRDVSGMELCFFSNSGAEANEAAIKLARLFGHQKNI
                     AEPAILVMENSFHGRTLATLSATGNKKVQVGFEPLVQGFIRVPFGDITAIEQKLAENP
                     NIVAILVEPIQGEGGIRTAPNGFTYLEELRAVCDKHDLLMMLDEIQTGNGRTGKYFAY
                     QHTAIKPDVVTTAKGLGNGFPIGACLVQGKASHLFKAGNHGSTYGGTPLGCRAALTVV
                     QLIQQGASDNAAVMGNRMITAFKEALCDLNVEVTGHGLMIGLRLPKPCAELVGIAREK
                     EKLLLNVTADCVVRLLPALIITAEEADEIVQRVVRLVREFLAQ"
BASE COUNT          315 a          205 c          258 g          360 t
ORIGIN      
        1 gcttttgaaa aaggtagcgg tgtttggttg tatgattctg atggaaacgc ttatttagat
       61 gctttaacag gtattgctgt gtgtggcctt ggccatgctc accctgtggt tgcagaggct
      121 attgctaatc aagctgcaac attagttcat acctccaatt tatatcaaat tcccttacaa
      181 gaagctttag gccaagcctt acgtgatgtt tcgggtatgg agttatgctt ttttagtaat
      241 tcaggtgcag aagccaacga agcggctatt aaattagcgc gtttatttgg tcatcaaaaa
      301 aatattgccg aacctgctat tttagtcatg gaaaactctt ttcatggtcg taccttagct
      361 accctttctg caacaggcaa taaaaaagtt caagtcggtt ttgaaccctt agtacaagga
      421 tttattcgtg tgccttttgg tgacattaca gccattgagc aaaaacttgc tgaaaatcca
      481 aacattgttg caattttagt agaacctatt caaggtgaag gtggcattcg cactgcacct
      541 aatggcttta cctatttaga agagttgcgt gcggtttgtg ataaacacga tttattaatg
      601 atgcttgacg aaatccaaac aggtaatggt cgtacaggta aatattttgc ctatcaacat
      661 actgctatta aaccagatgt cgtcactact gctaaaggtt taggtaatgg ctttccaatt
      721 ggtgcttgtt tagttcaagg caaagcgagc catttattta aagcaggtaa tcacggttcg
      781 acttatggtg gaacaccatt gggctgtcga gctgctttaa cggttgttca actcattcaa
      841 caaggtgcaa gcgataatgc cgctgtgatg ggtaatcgca tgattacagc atttaaagaa
      901 gcattgtgcg atttaaatgt agaagtgaca ggtcatggtt tgatgattgg cttacgctta
      961 ccaaagccct gtgccgaatt agttggcatt gctcgtgaga aagaaaaact cttgctgaat
     1021 gtgactgccg attgtgtcgt gcgtttatta cctgcattaa ttatcactgc tgaagaagca
     1081 gatgagattg tgcagcgtgt agtacgatta gtacgtgagt ttttagcaca ataatcgt
//
LOCUS       DAOQMB010000798         1138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116463_length_1138_cov_5.108033, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000798 DAOQMB010000000
VERSION     DAOQMB010000798.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116463_length_1138_cov_5.108033"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="PLY05_14045"
     CDS             <1..170
                     /locus_tag="PLY05_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HNC04461.1"
                     /translation="YGWQELARFININCFKNEPSIKSSLVFLRRTPWARQEVEQFYLK
                     YGHEDWLKDQS"
     gene            complement(167..>1138)
                     /locus_tag="PLY05_14050"
     CDS             complement(167..>1138)
                     /locus_tag="PLY05_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNC04462.1"
                     /translation="HGSITSNMLQTFAFLSTSLQIGRETMVCPLPLYHVFAFGLSCMG
                     MMLGMHTILITDPRDTQSVIDEMRKYPFTAMMGINSLFVALLNHPNIKTVDFSTLKLT
                     ISGGSLIQPHIARQWRLLTGCTVCDGYGMTEASPVIAMNPPQAIQEGTIGLPVSNTLI
                     RIVGEDNQNCPLGECGELWVKGPQLMKGYWRHAQATADTLTFDGWLKTGDIALIQPDG
                     YLRIVDRKKDIISINGFNVYPNEIETVLNEHPAILESAAVGMIDEQGNEAIKLFVVRK
                     ESTITELELFSYLSHQLAVYKIPQKIVFVESLPKSSIGKVLKRELKV"
BASE COUNT          361 a          251 c          196 g          330 t
ORIGIN      
        1 tatacggttg gcaagaactg gctcgtttta ttaacattaa ttgttttaag aatgaaccta
       61 gcatcaagtc gagtttagta tttttgcgtc gtactccttg ggcaagacaa gaagttgaac
      121 aattttatct aaaatatggc cacgaagatt ggttaaaaga tcaaagctaa acttttaact
      181 ctctctttag caccttacca atagaagact tcggcaaact ttccacaaaa acaatttttt
      241 ggggaatctt ataaaccgct aattggtgcg atagatacga aaacaactct agctctgtaa
      301 tagttgactc tttacgcacc acaaataatt taatcgcctc attgccttgc tcatcaatca
      361 tccccaccgc tgcactttct aaaatggcag gatgctcatt taacactgtc tcaatttcgt
      421 tgggataaac attaaaacca ttgatagaaa tgatatcttt tttacggtca acaatacgca
      481 aatagccatc gggttggatc agtgcaatat cgcccgtttt taaccagcca tcaaaggtta
      541 aagtgtctgc cgttgcttgc gcatgacgcc aataaccctt cattaattgt ggccctttga
      601 cccatagttc gccacactca cccaaaggac aattctgatt atcctcacca acaatacgaa
      661 ttaaagtatt cgaaactggc aaacctatgg tgccttcttg tatcgcttgc ggtggattca
      721 tcgcaatcac aggtgaggcc tcagtcatac cataaccatc acaaaccgtg catcctgtta
      781 ataatcgcca ctgtcttgca atatgcggtt gaattaaaga gccgccagaa attgtcaatt
      841 ttaaggtcga aaaatccacc gttttaatat tagggtgatt tagtagtgcc acaaataacg
      901 aatttatacc catcattgcc gtaaatgggt attttctcat ttcatcaatc accgattgag
      961 tatctctggg atcagtaatc agaatggtat gcattcctaa catcatgccc atacacgaca
     1021 aaccaaaggc aaagacatga tataacggca aaggacaaac catcgtttct cgaccaattt
     1081 gtaaactggt cgataaaaaa gcaaaagttt gtagcatatt actggtaata ctaccgtg
//
LOCUS       DAOQMB010000799         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116857_length_1136_cov_16.560592, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000799 DAOQMB010000000
VERSION     DAOQMB010000799.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116857_length_1136_cov_16.560592"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..>1136
                     /locus_tag="PLY05_14055"
     CDS             259..>1136
                     /locus_tag="PLY05_14055"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC04463.1"
                     /translation="MNYFVTGGTGFIGRFLVARLLKREDAVVYLLCRQSSQSKFFDLM
                     DELGATEQQLIPVFGDISQPSLVSDNDLAKLQGKIDHVFHLAAIYDMNMSEAEGEAIN
                     NQGTRNVVNFVNQLGGNVRLHHMSSIAVAGTEWQGVFKESMFDEGQALSHPYYRSKFT
                     SEKIVREESQVPYRIYRPGMVVGDSQTGIMDKVDGPYYFFKMIQRIRDNVPKWLPLLG
                     INGGQMPICPVDYVADATDYIAHKDGLDGRAFCLVQNPAPSVGEFMDIMFHAAHGPDF
                     AKKLDLPNIKMPSSAK"
BASE COUNT          336 a          198 c          233 g          369 t
ORIGIN      
        1 tttttacgca attaaaacaa acgccgattg taatcttaac aaaatcaacg tctagctttt
       61 tttagtattt ttgctctatt tttagtcacg actttttaca tttatttagt taaaaaacag
      121 aagattagaa tatctattct acagattact gctctataat cgctataata agaaaaacca
      181 taaaaaatat atggttttaa tcgcaataat aacaaagtta gcaaatccgc taactttcgg
      241 acgatgaggg ttatatagat gaactatttt gttactggtg gaactggttt tattggtcgt
      301 ttcttagttg cacgtttatt aaaacgtgaa gatgcggtgg tttatttatt atgccgtcaa
      361 tcttcgcaaa gcaaattttt tgatttaatg gatgagttag gtgctaccga acaacagctt
      421 attcctgtgt ttggtgatat ttctcaacct tcattggttt ctgataacga tttagccaaa
      481 ttacaaggca agattgacca tgtatttcac ttagccgcca tttatgacat gaacatgagt
      541 gaagccgaag gcgaagcgat taacaatcaa ggcacgcgca atgtggttaa ctttgttaat
      601 caattaggtg gcaatgttcg tttacatcac atgagctcga ttgctgtcgc tggcacagaa
      661 tggcaaggcg tgtttaaaga gtcgatgttt gatgaaggtc aagccttaag ccatccttat
      721 tatcgtagca aatttacctc cgaaaaaatt gtccgtgaag aaagccaagt gccgtatcgt
      781 atttatcgtc cgggtatggt ggtcggtgat tcacaaacag ggattatgga taaagtcgat
      841 gggccttatt acttctttaa aatgattcaa cgcattcgtg acaatgtgcc aaagtggtta
      901 ccgttattag gcatcaatgg cggtcaaatg ccaatttgtc ctgtggatta tgtcgctgat
      961 gcaactgact atattgccca taaagatggc ttagatggtc gtgcgttttg tttagtgcaa
     1021 aatcctgcgc cgagtgttgg tgagtttatg gacattatgt tccatgctgc tcatggtcca
     1081 gactttgcca aaaaattgga tttacccaat atcaaaatgc cgagcagcgc caaaaa
//
LOCUS       DAOQMB010000800         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116859_length_1136_cov_11.492137, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000800 DAOQMB010000000
VERSION     DAOQMB010000800.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116859_length_1136_cov_11.492137"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..>1136
                     /locus_tag="PLY05_14060"
     CDS             164..>1136
                     /locus_tag="PLY05_14060"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNC04464.1"
                     /translation="MISLKTIWPNTPSPYAHIEVTGLTADSRQVQQGFVFVALQGRQY
                     DARRFMTQAIEQGAVAVLCQSDKPSTEQQGIPIINIVDLPQQLGEIAARFYKQPSQDL
                     TLCAITGTNGKTSCAQLLAHACQFLGQKSAVLGTLGNGLVGELVPSTHTTLDALQLQQ
                     KLAEFKQAGAQVVALEASSHGLEQGRLTATKIDTAIFTNLTRDHLDYHGTMEAYQQAK
                     ALLFRWPSLKTAILNADDPVVKDFQAQLSTEVICWRYSQFPDSDAEFVALDIQPSLKG
                     LVINVKTPYGVANIHSPLLGRFNVSNLLAVLAGLLSIGVSLAQAVQAL"
BASE COUNT          331 a          250 c          247 g          308 t
ORIGIN      
        1 agtctctgca cctgtttttt cacgcgtgat gggtgaggcg ttacgattaa tgaatatccc
       61 tgtggataaa accctagagc caccaaaaaa caaagcaccc gttgtcacaa ctgcacccaa
      121 cgcgcctaaa ataacaaaca atcatcaagg agagcgtgct taaatgattt ccttaaaaac
      181 aatctggcca aacaccccta gcccgtatgc gcatattgag gtgacaggcc taacggctga
      241 tagtcgtcaa gtacaacaag gatttgtctt tgtggcttta caaggtcgcc aatatgacgc
      301 gcgtcgtttt atgacacaag cgatagagca aggcgcggtg gctgttttat gtcaaagcga
      361 taagccttca accgagcaac aaggtattcc catcatcaat attgttgatt tacctcagca
      421 attaggtgaa atcgccgcac gcttttataa gcagcctagt caagatttaa ctctctgtgc
      481 cattacaggt accaatggta aaaccagttg cgcacaatta ttagcgcacg cttgccagtt
      541 tttagggcaa aaatcggccg ttttaggaac attgggtaat ggtttagtgg gtgagttagt
      601 cccgtcaacg cacaccacct tagatgcctt gcaattacag caaaaactag ccgaatttaa
      661 acaggcaggc gcacaagtag ttgccttaga ggctagttct catggcttag agcaagggcg
      721 tttaacggct accaaaattg atacggccat ttttaccaat ttaactcgtg accacttgga
      781 ttatcacggc acaatggaag catatcaaca agcgaaagcc ttattatttc gttggccaag
      841 cttaaaaaca gccattctta atgccgacga ccctgtggtt aaagattttc aagctcaatt
      901 aagcacagaa gttatttgtt ggcgttatag ccaatttcca gatagtgatg ctgaatttgt
      961 tgccttagat atacagccct cgttaaaagg attggtcatc aatgttaaaa cgccttacgg
     1021 tgtggcaaac attcacagcc cattattagg ccgttttaat gtcagtaatt tattggcagt
     1081 gttggcaggt ttgctcagta ttggtgtgtc tttagcacaa gcggtacagg cattga
//
LOCUS       DAOQMB010000801         1136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_116861_length_1136_cov_8.415356, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000801 DAOQMB010000000
VERSION     DAOQMB010000801.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116861_length_1136_cov_8.415356"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLY05_14065"
     CDS             <1..292
                     /locus_tag="PLY05_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNC04465.1"
                     /translation="YALFLPSGLSQVSWQVIALQAAFQGILVVIVAMLTFIAAVQRLG
                     AFTVGSFLALAPMLAATLAVPVLDEPLTLGLVLGLIGMVTGALQPWRLLKKS"
     gene            306..728
                     /locus_tag="PLY05_14070"
     CDS             306..728
                     /locus_tag="PLY05_14070"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNC04466.1"
                     /translation="MKFSEKRLGQIFDRTSGYCHICHAKLDFDRYGDVGRSGSWEVEH
                     STPQAKGGTHHLNNLYPACISCNRAKSDNSTRSARAKHGKKRAPLSVKKRRQAKIING
                     MKGSGLGVIATAIISIDVSGGFIVLGFVIGYLLNPDKH"
     gene            836..>1136
                     /locus_tag="PLY05_14075"
     CDS             836..>1136
                     /locus_tag="PLY05_14075"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNC04467.1"
                     /translation="MMSEISYQILGSDLQYVEVTLPPQCSVIGEQGAMMYMDNYLEVK
                     TILGDGNGLFSAIKRSLTGESLFSSLYTNNNNFAQRVAFAAPTVGKIIAINLAEVG"
BASE COUNT          312 a          194 c          265 g          365 t
ORIGIN      
        1 ttacgcgctg tttttgccca gtggtttaag tcaagtctct tggcaagtca ttgccttgca
       61 agctgctttt caaggtattt tagttgttat tgtagcaatg ctaaccttta ttgctgcggt
      121 acagcgttta ggggcattta ccgtaggtag tttcttggct cttgcaccca tgcttgcagc
      181 aacgttggcc gtacctgtgt tagatgaacc attaacctta gggttagtac tcggtttgat
      241 tggcatggtt acaggtgcgt tgcagccgtg gcgattgcta aaaaagtctt aataatcagt
      301 taattatgaa gttttccgaa aagagattag ggcaaatttt tgaccgtacg tcaggctact
      361 gtcatatttg tcatgctaaa ttagattttg atcgttatgg tgatgttggc cgcagcggtt
      421 cgtgggaggt tgaacactca actccacaag ccaaaggtgg cacacatcat ttaaataatt
      481 tgtatcctgc ttgtattagt tgtaatcgtg cgaaaagtga caatagtaca cgctcagcac
      541 gagctaaaca tggcaaaaaa cgcgcacctt tatcggttaa aaaacgccga caggcaaaaa
      601 taatcaatgg tatgaaaggt tctggcttag gcgtaattgc tacggcaatt attagtattg
      661 atgtttcagg tggttttatt gtgttaggct ttgtgattgg ttatttatta aatcccgata
      721 aacattaaag tcaaacgtga ttgattagta agatgattgc ccttcgactt cgctcaggga
      781 gcgtaatcga aaccacttca attcgctcag tgtgcgacta aataataaag gaacgatgat
      841 gtcagagatt agttatcaaa ttctgggttc agatttgcag tatgtagaag taactttgcc
      901 accacaatgt tcagtgattg gtgagcaagg tgcgatgatg tatatggata attatttaga
      961 agtcaaaacc attttaggcg atggtaatgg tttgtttagt gccataaaac gctcattaac
     1021 gggcgagtcg ttatttagta gtttgtatac taataacaat aattttgctc agcgtgtagc
     1081 atttgctgcg ccaactgttg gcaaaatcat tgctatcaac ttagcggaag ttggtg
//
LOCUS       DAOQMB010000802         1135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_117060_length_1135_cov_19.158333, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000802 DAOQMB010000000
VERSION     DAOQMB010000802.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117060_length_1135_cov_19.158333"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /gene="dapC"
                     /locus_tag="PLY05_14080"
     CDS             <1..1102
                     /gene="dapC"
                     /locus_tag="PLY05_14080"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HNC04468.1"
                     /translation="LSIGEPKHPSPRFVIDIINQQAKHLATYPSTKGIAELRQAIADW
                     LLQRFKLSRVDAENQVLPVNGTREALFSFTQAVVNRQHDKPLVVMPNPFYQIYEGAAY
                     LAGAEPYYLSCHAHNQFSPDFDSVPAEVWQRTQLLFICSPANPTGTVLDKQTLSKLIA
                     LSDQYGFVIASDECYSEIYFDENNPPLGLLQVCAELGRHDYKNCVVFHSLSKRSNLPG
                     LRSGFVAGDANILKPYLLYRTYHGSAMPVQHQYASVAAWQDEQHVIENRGQYRQKFAE
                     FYAIVNPVLPLTMPDAGFYFWAKVPDGFTDETFAQFLYEKAHITVLAGRYLGRTDHLG
                     QNPAENYVRMALVAPVADCVDAAQRIVNLLKS"
BASE COUNT          283 a          255 c          237 g          360 t
ORIGIN      
        1 tttgtcgatt ggtgagccaa aacatccttc ccctcgtttt gtcattgaca ttattaacca
       61 acaagccaag catttagcga cttacccaag cactaaaggc attgccgaat tgcgtcaggc
      121 gattgctgat tggttattgc aacgctttaa gctgagccgt gttgatgccg aaaaccaagt
      181 attaccagtg aatggtacac gcgaagcgtt gttctcgttt actcaggcgg tcgtgaatcg
      241 tcaacacgat aaacctttag tggtgatgcc caatccgttt tatcaaattt atgaaggtgc
      301 ggcctattta gcaggtgcag agccgtatta cttatcgtgt catgctcata accaatttag
      361 ccctgatttt gacagcgtac ctgctgaagt ttggcagcgc acccaattat tatttatttg
      421 ctcccctgcc aatccaacag gtactgtttt agacaaacaa accttaagca agttaattgc
      481 actcagtgat caatatggct ttgtcattgc ctctgatgag tgttattccg aaatttattt
      541 tgacgaaaac aacccaccct taggtttatt gcaagtttgt gctgaattag gtcgccacga
      601 ttataaaaat tgtgtggtgt ttcattcttt atccaaacgc tcaaatttac ctggtttacg
      661 ctcgggtttt gttgctggtg atgccaatat tctcaagcct tatttgttgt atcggacgta
      721 tcatggttca gccatgcctg ttcaacatca atatgcgtct gtggcggcgt ggcaggatga
      781 acaacacgtt attgaaaatc gtggccagta tagacaaaag tttgctgaat tttatgctat
      841 cgttaaccct gttttaccac tcaccatgcc cgatgcaggt ttctattttt gggcgaaagt
      901 gcctgatggt tttaccgatg aaacctttgc tcagttctta tacgaaaaag cccatattac
      961 cgtattggct ggtcgttatt taggtcgcac tgaccatctt ggccaaaacc ctgccgaaaa
     1021 ttatgtgcgt atggccttgg ttgcccctgt tgctgattgt gtggatgctg cacagcgaat
     1081 tgttaatctt ttaaaaagtt gattttaccc tcaccccaac cctctcccaa agggc
//
LOCUS       DAOQMB010000803         1134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_117423_length_1134_cov_2.061168, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000803 DAOQMB010000000
VERSION     DAOQMB010000803.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117423_length_1134_cov_2.061168"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PLY05_14085"
     CDS             complement(<1..1012)
                     /locus_tag="PLY05_14085"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HNC04469.1"
                     /translation="MINGQLKSKIDKLWEEFWTGGITNPLTVIEQITFLIYARLLDMN
                     ETSDEKKALRTKKEFKRRFNEQQQHIRWHHLKHIESADVLMTTVRDHLFPYFKSTAAS
                     NSLFAEFMKDAQLMVQKPSLLVKAIAMVDELPLDNGDTKGDLYEYLLSKLTTAGINGQ
                     FRTPRHIIRAMVELLDPTATDRICDPACGTAGFLSVAYEFMLQKYSSAAGTHQETWIN
                     EKGETETQTLYSGDLLAEHRHHVDTDMFHGFDFDATMLRIAAMNLVMHGVAEPDIHYQ
                     DTLSQRFGEKYPQAAKEGFDIILANPPFKGSLDEGDVAPDLLAIVKTKKTELLFVALI
                     LRM"
BASE COUNT          329 a          233 c          213 g          359 t
ORIGIN      
        1 acatgcgtaa aatcaaggcc acaaacagca attcggtttt tttagtttta acaatcgcca
       61 gtaaatcagg tgcaacatcg ccctcatcta aactcccttt aaacggcgga ttagctaaaa
      121 taatatcaaa accttcctta gcagcttgtg gatacttttc gccaaaacgt tggcttaaag
      181 tatcttgata atgaatatca ggctctgcaa ctccgtgcat caccaaattc atggccgcaa
      241 tacgtaacat agtggcatca aagtcaaaac catgaaacat atcggtatca acatgatgac
      301 gatgctccgc caacaagtcg ccactgtata aggtttgagt ttcggtttcg cctttttcgt
      361 taatccacgt ttcttggtgt gtgcctgcgg ctgagctata tttttgcaac ataaactcat
      421 aagcaacact taaaaatcct gctgtaccac aagcagggtc acaaatacga tctgttgctg
      481 tggggtctaa tagttcaacc atggcacgaa taatgtggcg tggtgttcta aattggccgt
      541 taatccctgc ggtggtgagt ttgcttaata agtattcgta taaatcccct ttggtatcgc
      601 cattatcaag cggtaactca tcaaccatcg caattgcttt taccaacaac gaaggctttt
      661 gtaccatcag ttgagcatct ttcataaact cagcaaataa gctgttgctg gctgcggtgc
      721 ttttaaagta aggaaataag tggtcacgaa ccgttgtcat taacacatcg gcagactcaa
      781 tgtgtttaag atgatgccaa cgaatatgtt gttgctgctc gttaaaacgc cgtttaaatt
      841 cttttttagt gcgtaaggct tttttttcgt cactggtttc gttcatgtct aataaacggg
      901 cataaatcaa aaaagtgatt tgctcaataa cggttaaggg attggttata ccacctgtcc
      961 aaaattcttc ccacaatttg tctattttgc tttttagttg accgttaatc ataaatactc
     1021 tgttatcaaa tggatatgtg ataaatgaaa tgaattatca atagtctatc attaaatgct
     1081 tgtttaatga gttgcccctc attaatagct ttcaatcatg gcttgttgac gatg
//
LOCUS       DAOQMB010000804         1133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_117478_length_1133_cov_9.427644, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000804 DAOQMB010000000
VERSION     DAOQMB010000804.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117478_length_1133_cov_9.427644"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /gene="hslU"
                     /locus_tag="PLY05_14090"
     CDS             <1..407
                     /gene="hslU"
                     /locus_tag="PLY05_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="heat shock protein involved in degradation of
                     misfolded proteins; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HNC04470.1"
                     /translation="KPSDLIPELQGRLPIRVELNALTPSDFERILTEPHFSLTEQYKA
                     LLKTEGLNIEFSPDCIQRLAEVAFEVNERTENIGARRLHTVLERLLDEISYNAGDLGA
                     ERQGDVLILSSEDVNKQLGNLVQDEDLSRFIL"
     gene            578..832
                     /locus_tag="PLY05_14095"
     CDS             578..832
                     /locus_tag="PLY05_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04471.1"
                     /translation="MSSFTITPKDSLEATLDRVKTTISSKGGQFTGDTNNGQFSGNTP
                     VGAVKGKYVVKNGSLEITITDKPFLAPMSIIEDKIRNYFA"
     gene            914..>1133
                     /locus_tag="PLY05_14100"
     CDS             914..>1133
                     /locus_tag="PLY05_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04472.1"
                     /translation="MTREEITTTEGILRYDLARYRAWRCHSDEQFKQWFNAVQKWQVD
                     RLTRTHGHLLEDERYQAVTNFFLSDMYGG"
BASE COUNT          346 a          217 c          206 g          364 t
ORIGIN      
        1 ctaagccttc cgatttaatt cctgaacttc aaggtcgttt gccgattcgt gttgagctaa
       61 atgccttaac gccttctgac tttgaacgca ttctgaccga gccgcacttc tctttaaccg
      121 aacaatataa agccttgtta aaaaccgaag gcttaaatat tgagtttagt ccagattgta
      181 ttcaacgttt ggctgaagtg gcctttgaag tcaacgaacg tactgaaaat attggcgcac
      241 gccgtttaca taccgtgtta gaacgtttat tagatgaaat ttcctataat gcgggtgatt
      301 tgggtgcaga acgtcaaggt gatgtcttaa ttttaagttc tgaagacgtc aacaaacaat
      361 taggcaattt agtgcaagat gaagatttaa gtcgctttat cttataattt cgctacgtca
      421 acaaccttta gttttctctc taaacaccaa ggtcttagag agaaaactaa aacattctac
      481 tttttaatga atttttgtta aaatagcccg cctgtatctg aggtaaatta ttcatcatcc
      541 tcatcatatt aaaatttttt tatatctagg ttcaattatg tctagcttta ctattacccc
      601 taaagatagc ttagaagcga ctttagaccg cgtaaaaaca acaatcagca gcaaaggcgg
      661 tcaatttact ggcgatacca ataatggcca attttctggg aacacaccag taggtgcagt
      721 aaaagggaaa tatgtggtaa aaaatggtag tttagaaatc accattactg acaaaccctt
      781 tttagcccca atgtcgatta ttgaagataa aattcgcaat tattttgctt aaattaacaa
      841 cataaaactg acactttatt ttagtgtatt gtgctgtttt gtgttttttt tatcctaaac
      901 tcaaggtttg accatgacac gcgaagaaat taccactacc gaaggcattc tccgttatga
      961 cctcgctcgt tatcgtgctt ggcgttgcca ctctgacgaa caatttaaac agtggtttaa
     1021 tgctgtacaa aaatggcaag tggatcgttt aactcgcact catggtcact tactagaaga
     1081 tgaacgttat caagcagtca ccaacttctt tttgagtgat atgtatggtg gct
//
LOCUS       DAOQMB010000805         1132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_117692_length_1132_cov_12.132776, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000805 DAOQMB010000000
VERSION     DAOQMB010000805.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117692_length_1132_cov_12.132776"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1132
                     /locus_tag="PLY05_14105"
     CDS             <1..>1132
                     /locus_tag="PLY05_14105"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HNC04473.1"
                     /translation="LLADKVEALLKALPKAIRRQLVPIPDTVKGLLSQLPESRQQSLT
                     QVLGGLLQRRGITINAADWQEAENNLPFHCRFYIEVIDNKGKVLKTGRDLSTLKIQLS
                     SQKVELAVNNQQILTSFPERIEPVVEKTVAGLPTRSYAALVKQEQGVTLQYLANQHLA
                     HTQHQRGCLYLWQLHCATELKQFKKSIPNALVLDFSAFGNNKAQLEQQLVDALCWQVF
                     GEQLVYSQVDFDKVLTSKRGQLLTMGQQLIKTVANIYQTWRAINQQIRLYGQAVFAQA
                     IADINLQLTNLQAQSFLSEIAPQRWQEYPRYLKGLQLRLERLTNNIQRDNQASQELHK
                     RWQQYQQKVADFTARSVNIQPLHDYRWLLEEYRISLFSQPMKT"
BASE COUNT          364 a          214 c          231 g          323 t
ORIGIN      
        1 cattgttagc cgataaagtt gaagccttat taaaagcctt gcctaaagcc attcgtcgtc
       61 aattagtgcc tattcctgat acggttaagg gcttgttaag ccaattacct gagagtcgtc
      121 aacaatcgtt aacacaagtt ttaggtggat tgttgcaaag acgtggtata acaattaacg
      181 ccgcagattg gcaagaagcc gaaaataact tgccatttca ctgtaggttt tatatagaag
      241 ttattgataa taaaggaaag gtattaaaaa caggacgtga tttatctacc ttgaaaattc
      301 agctttctag ccaaaaggtt gagctggcag tcaataacca acaaatctta accagctttc
      361 ctgaacgcat tgaaccagtt gtcgaaaaaa cggtggcagg tttacccacg cgtagttatg
      421 ccgctttagt caaacaagag cagggtgtga ctttgcaata tttggctaat caacatttgg
      481 ctcacaccca gcatcaacgt ggctgtttat atttatggca attacactgt gcgactgaat
      541 taaaacagtt taaaaaatct attcccaatg ctttggtatt agatttctct gcgtttggca
      601 ataataaagc tcaactagag cagcagttgg tggatgcctt gtgttggcaa gtctttggtg
      661 aacagttagt gtatagtcag gttgattttg acaaagtatt aaccagcaaa cgtggccaat
      721 tattaacgat ggggcaacaa ttaatcaaaa ccgtagctaa tatttatcaa acatggcgag
      781 cgattaatca gcaaattcgt ttatatggtc aagcggtatt tgctcaagca attgccgaca
      841 taaacttaca actgactaat ttacaagctc agagcttttt aagtgaaatc gcgccccagc
      901 gttggcagga atacccacga tatttaaaag gtctgcaatt acgtttagaa cgattaacca
      961 ataatataca gagagataat caggccagcc aagaattgca caaacgttgg cagcaatatc
     1021 aacaaaaagt tgctgacttt acagcacgga gtgttaatat tcagcctctg cacgattatc
     1081 gttggttgtt agaggaatat cgaatctctt tgttttcgca accgatgaaa ac
//
LOCUS       DAOQMB010000806         1129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_118306_length_1129_cov_9.584730, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000806 DAOQMB010000000
VERSION     DAOQMB010000806.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118306_length_1129_cov_9.584730"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..1025
                     /gene="nuoH"
                     /locus_tag="PLY05_14110"
     CDS             24..1025
                     /gene="nuoH"
                     /locus_tag="PLY05_14110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016657324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HNC04474.1"
                     /translation="MFDWLTPEIVATLVAVAQAVVILLATVILAALLSFIERRLLAWW
                     QDRYGPNRVGPYGMFQIAADMLKMFFKEDWTPPFADKLTFRLAPAIAMGTVLLSMAVV
                     PITPTWGVIDLNIGLLFFFGMAGLAVYSVMFAAWSSNNKYSLLGGLRAAAQTLSYEVF
                     MGLALMGVVAQAGSFNMREIVEAQQGMWNIIPQFLGFCTFLVAGIAVTHRHPFDQPEA
                     EQELADGYHIEYSGMKWGMFFVGEYVAVVVVSAMLTTLFLGGWLPLFSFLDFIPPFLW
                     FAGKTAFFIMLFVLVRGSLMRPRYDQVMKFSWLFCLPVTLLNLLVTAAVILTWGSAA"
BASE COUNT          244 a          219 c          283 g          383 t
ORIGIN      
        1 acagtaacag gggagggcgc ataatgtttg attggcttac gcccgaaatc gtagcgacct
       61 tggtcgctgt tgcacaagcc gtggtgattt tgcttgccac ggttatttta gcggctttat
      121 taagttttat tgagcgtcgt ttattggctt ggtggcaaga ccgttatggc ccaaaccgtg
      181 ttggccctta tggtatgttc caaattgctg ccgacatgct caaaatgttc ttcaaagagg
      241 actggacacc gccctttgcg gataagttaa cctttagatt agcaccagcg attgccatgg
      301 gaaccgtgtt gttgtcgatg gcggttgtac cgattacacc cacatggggg gtaattgatt
      361 taaacattgg tttgttattc ttctttggta tggcaggctt agcggtttat tccgtgatgt
      421 ttgcggcatg gtcatctaat aacaaatact cgttattagg tggtttacgt gctgccgcac
      481 aaaccttgag ttatgaagtg tttatgggct tagccttaat gggtgttgtt gcccaagctg
      541 gctcgtttaa tatgcgtgaa attgttgaag cccaacaagg tatgtggaac attattccgc
      601 agttcttggg cttttgtacc ttcttagtcg caggtattgc ggtaacgcac cgtcatccgt
      661 ttgaccaacc cgaagccgaa caagaattgg ctgacggtta tcatattgaa tactctggta
      721 tgaaatgggg tatgttcttc gttggtgaat atgtggcggt agttgtggtt tcagccatgt
      781 taacgacctt gtttttgggt ggttggttgc cattattttc cttcttagat tttattccgc
      841 catttctttg gtttgctggt aaaactgcct ttttcatcat gttatttgtg ttagtccgtg
      901 gctcgttgat gcgtccgcgt tatgaccaag tgatgaaatt tagttggtta ttttgtttgc
      961 ccgtcacgtt actcaatctt ttagtaaccg ccgccgtgat tttgacatgg ggttcagcag
     1021 catgaattta atttctatta tcaaaggcgc agggacacaa gtccgcagta tgcttatggt
     1081 atttagtcat ggctttcgta agcgtgacac cattcaatac ccagaagaa
//
LOCUS       DAOQMB010000807         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_118522_length_1128_cov_16.681267, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000807 DAOQMB010000000
VERSION     DAOQMB010000807.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118522_length_1128_cov_16.681267"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..>1128
                     /locus_tag="PLY05_14115"
     CDS             260..>1128
                     /locus_tag="PLY05_14115"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNC04475.1"
                     /translation="MDRLDLMTIFVAVAEAESFSGGARRLAMSPPAVTRAISALESRL
                     AVKLLDRTTRYVRVTELGQRYLDDARRIIAEVDEAEDALVGINGTPKGQLTLTAPVLF
                     GKMFVMPVVVEYLQRYPSMDVSALFLDRVVNMMEEGVDVGIRVGELPDSSLKAVRVGQ
                     IRRVLCASPQYLAEYGVPNHPHDLQQHRIVAATGVTPSVEWKFWQEQQALSVRLRPRL
                     TVTTNDGAIESALAGFGVTRLMSYQIAHFVESGQLQILLPDFESAPLPINVLHREGRH
                     KSARVRSFVDLVV"
BASE COUNT          278 a          198 c          289 g          363 t
ORIGIN      
        1 agtgttaata cggctcatga ttttttcctc ggttaggttt gttggtgtgg ctatatagga
       61 ctgacgcggc tatcgcttat ttgttcacta aaaaatcgtt tttaacgatt tttagttcac
      121 aaaacgcgct aatttcggtg aaatgcgtca gtcccttata gtgatatagt ttgttttttt
      181 aaagaatcct tgttttaggt aattatttat tgcctaaaat ggaataatga tggtattaaa
      241 gataaaaaag gggaaatata tggatcgttt agacttaatg acgatttttg tcgctgttgc
      301 tgaggccgaa agtttttcgg gtggagcaag acgtttggcg atgtcaccac ctgccgtcac
      361 cagagccatc tcagctttag aaagtcggtt ggcggttaaa ttattagata gaacaacacg
      421 ttatgttcga gtaaccgagt taggtcaacg ctatttggat gatgctcggc gtattattgc
      481 cgaagttgat gaagccgaag atgccttggt tggcattaat ggcacgccca aagggcaatt
      541 aaccctaacc gcacccgtct tatttggcaa aatgtttgtc atgcctgtgg ttgttgaata
      601 tttacaacgt tatcccagca tggatgtcag tgccttgttt ttagatcgtg tggttaacat
      661 gatggaagaa ggggtagatg tgggcattcg ggttggtgag ttacctgatt ccagtttaaa
      721 ggcggtgcgt gtcgggcaaa tccgtcgggt gttatgtgcc tcaccacaat atttggcgga
      781 atatggtgtg cctaatcatc ctcatgattt gcaacagcat cgaattgttg cggcaacggg
      841 tgtgacaccc agcgtggaat ggaaattttg gcaagagcaa caagctttgt cggtacgttt
      901 gcgaccacgt ttaacggtaa ccaccaatga tggtgcgatt gaatctgcct tagcaggctt
      961 tggggtgaca cgtttaatgt cgtatcaaat tgcgcatttt gttgagagtg gtcagttaca
     1021 gattttatta cccgattttg agtctgcacc cttaccgatt aatgttttgc atcgtgaagg
     1081 tcggcataaa tcggcgcgtg tgcgtagttt tgtcgattta gtcgttga
//
LOCUS       DAOQMB010000808         1128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_118524_length_1128_cov_10.339236, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000808 DAOQMB010000000
VERSION     DAOQMB010000808.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118524_length_1128_cov_10.339236"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            402..>1128
                     /locus_tag="PLY05_14120"
     CDS             402..>1128
                     /locus_tag="PLY05_14120"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNC04476.1"
                     /translation="MSAPISAHISPKTYWMGAACVALSALLFSTKSVFVKMAYQYPID
                     STILLALRMAFALPFFVLMLWFNRSTTPIKANRQHYIGLVIAGVVGYYGASIFDFWGM
                     EYISASLERLILFMYPTLTVILSALFLKRAINRSTCLAIVISYAGMCLVFLGNQHEES
                     TLFRTNAVIAYLLTLQPFATVLCSQNALIAVQCVSPELVLGSSLVFAGALAYAGYLVG
                     SGVLIPQFGAVRFTAFALMIATVC"
BASE COUNT          275 a          226 c          240 g          387 t
ORIGIN      
        1 attttttgac ggtatcaggc gtagctgcaa tgcgtgtttc gccagcgatg atttccgctg
       61 gaattccgat ttgcatgatc aaccctcgag ctgagtgtga caaactaggc ttattgaccg
      121 ctcgttttga gcataacaat caaataaacc cacaggttta ggacttacgt ggtggtgagc
      181 acgcacaacg gcgatgcaaa acgtaagtca taaggtatgg ctagtcattt gagcttgtga
      241 ctcactagcc cccacaaaaa aataaaaagg cgcgcgattg tactctaagt tgaccaatat
      301 ttttagtatg aataccaaca ttttctattt ttttaggcca ttgccttgca aaaaatcttg
      361 ttttaagctt atcgacttat tctttttaga ctgtttacgc catgtctgcg ccaatttctg
      421 cccatatctc tcctaaaacc tactggatgg gtgcagcttg tgtcgcatta tctgcccttt
      481 tattctcaac caaatctgtt tttgtcaaaa tggcttatca atatccgatt gatagcacga
      541 ttttattagc tttaagaatg gcttttgccc tgcccttttt tgtgttgatg ttatggttta
      601 atcgcagtac aacacctata aaagccaatc gccaacatta cattggttta gtgattgcgg
      661 gtgtcgttgg ttattatggg gcaagtattt ttgatttttg gggcatggaa tacatcagcg
      721 caagcttaga acggctgatt ttgtttatgt accccacctt aacggtgatt ttatcggctt
      781 tatttttaaa acgtgctatt aatcgcagca cctgtttagc cattgtcatc agttatgcag
      841 ggatgtgttt ggtcttttta ggtaaccaac acgaagaaag cacacttttt aggactaacg
      901 cggttatcgc ttatttactc acattgcaac cgtttgcaac ggttttatgt tcacaaaacg
      961 cgctaattgc agtgcaatgc gttagtcctg aactggtttt aggcagtagt ttggtatttg
     1021 caggggcttt agcctatgca ggctatttag tgggcagtgg cgtattaatt cctcagtttg
     1081 gcgcagtgcg ttttactgct tttgccttga tgattgccac tgtttgtt
//
LOCUS       DAOQMB010000809         1127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_118726_length_1127_cov_13.895522, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000809 DAOQMB010000000
VERSION     DAOQMB010000809.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118726_length_1127_cov_13.895522"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..454)
                     /gene="infA"
                     /locus_tag="PLY05_14125"
     CDS             complement(233..454)
                     /gene="infA"
                     /locus_tag="PLY05_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNC04477.1"
                     /translation="MSNKEEQIEMDGTVVDTLPNTMFRVQLENGHIVTAHISGKMRKH
                     YIRILTGDQVKVEMTPYDLTKGRITYRVR"
     gene            complement(609..>1127)
                     /locus_tag="PLY05_14130"
     CDS             complement(609..>1127)
                     /locus_tag="PLY05_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HNC04478.1"
                     /translation="KTQLLAFLLAARFRQPIPIVLGIFVATIINHAFAGAVGMYLNSL
                     IGQDNMRWILGIGFLIMAAWLLIPDELDEQQEQDSSRFGVFGTTVIAFFLAEMGDKTQ
                     IATVALSAKYAADFVWVILGTTLGMMLANVPAVYLGNHFAQKLPIRWIHGIAASIFAL
                     IGILILLGKTGF"
BASE COUNT          365 a          212 c          227 g          323 t
ORIGIN      
        1 atggctcgcg ttattcaaga aaatctgaaa aagcctttag ctgaacagat tttatttggt
       61 gacttggtgg gtggtggtca tgtgcatgta acggttgaga atgatgcgat cgttctccat
      121 gttgaagcaa ctgagaaaaa gacagcagcg cctgcttaag gtaaaatttc ttaacaaagc
      181 aaaagagggc ttgacgtata acgtaagccc ttttttatat taagccggtc gattaacgta
      241 cgcggtaagt aatacggcct ttagtcaagt cataaggtgt catttcaact ttgacttggt
      301 cacctgttaa aatacgaata tagtgtttac gcattttacc agagatgtgt gcggtcacaa
      361 tatgaccatt ttctaactgc acacgaaaca tggtgtttgg taaagtgtcg accactgtac
      421 catccatctc aatttgctct tctttatttg acatagcaac cctagattgt aaaaaattca
      481 agatttatgc ggacacaaag gggaatatat gtttgcccaa tgacatagcg catattttaa
      541 ggtggcgtat tttgcctgat ttttaacaaa agtaaaacaa ctttaggttt taatcttgca
      601 tctttacatt aaaagcctgt tttccctaat aagattaaaa tgccaattaa ggcaaaaata
      661 ctagcagcaa taccatgtat ccagcgtatt ggtaattttt gtgcaaaatg attgcccaaa
      721 tacacggctg gaacgttggc tagcatcatg cctaatgttg tgccaagaat cacccaaaca
      781 aaatcagcgg catatttagc acttaaggcg acagtggcaa tttgagtttt atcacccatt
      841 tctgccaaaa agaaggcaat gacagtggtg ccaaacacgc caaaacgtga ggagtcttgt
      901 tcctgttgtt cgtctaattc atctggaata agtagccaag cagccatgat taaaaaacca
      961 atgcctaaaa tccaacgcat attgtcttgg ccaattaagc tatttaaata catacctact
     1021 gcacctgcaa aagcatgatt tataatagtt gcaacaaaga tacccaaaac aatggggata
     1081 ggttggcgaa aacgtgcagc taacaaaaag gcaaggagtt gggtttt
//
LOCUS       DAOQMB010000810         1126 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_118930_length_1126_cov_18.202614, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000810 DAOQMB010000000
VERSION     DAOQMB010000810.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118930_length_1126_cov_18.202614"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1121
                     /locus_tag="PLY05_14135"
     CDS             15..1121
                     /locus_tag="PLY05_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HNC04479.1"
                     /translation="MSGTGLFTPEESISNEELIDSFNAYVELFNAKNADAIERGEVTA
                     LKTSFPEFIEKASGIKSRYVINKSGILDPERMFPVIADRSDDQQSLQCEMAVKAIEGA
                     LAQSGKTAADIDAVLVACSNMQRPYPAMSIEIQHAIGAKGFAFDMNVACSSATFGLQT
                     AADMVRSGSAKCVLMVNPEICSGHLEWRDRDCHFIFGDVCTAVIIETADTCTNPEAWE
                     ILGTKLQTQFSNNIRNNFGFLNRCDEEGVGKRDKLFRQEGRKVFKEVCPMVAEQITQH
                     LGELEISATDLKRMWLHQANSNMNDLIARKVLGRDPSPEESPTILDTYANTSSAGSII
                     AFHLHRNNLNANDIGVICSFGAGYSIGSVIIKKR"
BASE COUNT          323 a          210 c          248 g          345 t
ORIGIN      
        1 tgaaaaaagt cgtcatcagt ggtacaggtt tattcactcc tgaagaaagt attagcaatg
       61 aagagttaat tgactcattt aatgcctatg ttgaattatt taatgccaaa aatgctgatg
      121 ccattgagcg cggtgaagtc acggcgttaa aaacctcttt tcccgaattt atcgaaaaag
      181 catcaggcat taagtctcgt tatgtcatca ataagtcagg tattttagac ccagaacgta
      241 tgtttccagt gattgctgat cgttctgatg atcaacaatc cttacaatgt gaaatggctg
      301 ttaaggcaat tgaaggggca ttagcacaat caggcaaaac cgcagcggat attgatgcgg
      361 tattggttgc ttgctcaaat atgcagcgtc cttatcctgc catgtccatc gaaattcaac
      421 atgcgattgg tgcaaaaggt tttgcttttg acatgaatgt cgcttgttct tcggcaactt
      481 tcggtttgca aactgctgct gatatggtgc gtagtggctc agcaaaatgc gtgttaatgg
      541 ttaacccaga gatttgttcg ggtcacttag aatggcgcga tcgtgattgc cattttattt
      601 ttggtgatgt gtgtaccgcg gtgattattg aaacagccga tacttgcacc aatcctgaag
      661 catgggaaat tttaggcact aaattacaaa cccaattctc taacaatatt cgcaacaact
      721 ttggtttttt aaatcgttgt gatgaagaag gcgttggcaa acgcgataaa ttattccgtc
      781 aagaaggccg caaagtcttt aaagaagttt gcccaatggt tgctgagcaa attacccagc
      841 atttaggcga attagagatt agtgcgaccg atttaaagcg tatgtggttg catcaagcca
      901 atagcaatat gaatgattta attgcccgta aagtattggg acgtgatcca agtcctgaag
      961 aatcacccac gattttagat acttatgcca acaccagttc cgcaggttct attattgcct
     1021 tccatttgca ccgcaataat ttaaacgcca atgatattgg tgtgatttgt tcctttggtg
     1081 caggttactc gattggtagt gtgattatta aaaagcgtta atcacg
//
LOCUS       DAOQMB010000811         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_119170_length_1125_cov_8.152336, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000811 DAOQMB010000000
VERSION     DAOQMB010000811.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119170_length_1125_cov_8.152336"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1125
                     /locus_tag="PLY05_14140"
     CDS             <1..>1125
                     /locus_tag="PLY05_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04480.1"
                     /translation="GILKIHQSMSDKDIPAEALRSIKAYHGSPAMFDKFMREFVNSGE
                     GAQAFGWGHYFAGRKALAEWYRTVLSNDFNRTSFVFRKDGERVRLTDLYTPKSEWEKV
                     NEDELVELFKKYLLGKQGERGKTWQADTDPRYDALAEIAKDLSQYNKDTIGYAELKAV
                     QQVMQKYKSKQFSAQRVSTAHNKTGQLYEVDIAAKEESFLDWFTKFDDLSPAEQKSLA
                     KVFKEFDAYGDLDATDKTIGQLYQKVTRYSQDGTPKRLSLALQDLGYSGIKFLDQESR
                     KNPYEKQYFNYVVFDENIISIKDTYYSKQQGATEGFYLNGTVHLIADNLTDETIIPTF
                     VHELGGHKGFQEMMSPKAYELIMQQFDRLVAQGNAIAIKA"
BASE COUNT          393 a          194 c          238 g          300 t
ORIGIN      
        1 aaggtattct taaaattcat cagtcgatga gtgataaaga cattcctgct gaggcattga
       61 gaagtattaa agcctatcat ggcagccctg caatgtttga caaatttatg cgtgagtttg
      121 ttaattcagg tgaaggcgca caggcttttg gatgggggca ttattttgca ggaagaaaag
      181 cattagccga atggtacaga acagtgctgt ctaatgattt taacagaaca tcgttcgttt
      241 ttagaaaaga tggtgagcga gttcgcctga ctgacttata tacacctaaa tctgaatggg
      301 aaaaggttaa tgaggatgaa ttagtagaat tattcaaaaa gtatttactt ggaaaacaag
      361 gcgaaagagg caaaacatgg caagctgata cagaccctag atatgatgct ttagctgaaa
      421 ttgctaagga tttatcacag tacaacaaag acacaattgg ttatgctgaa ttaaaagctg
      481 tgcagcaagt gatgcaaaag tacaagtcca aacagttttc ggctcaacgg gtatcaaccg
      541 cgcacaacaa aacaggccaa ttatatgaag tagatatagc agccaaagag gagtcttttt
      601 tagattggtt tactaagttt gatgacttat cacccgcaga acaaaagtca ttagcaaagg
      661 tatttaaaga gttcgatgct tacggtgatt tagatgcgac agataaaacg ataggccaac
      721 tttatcaaaa agtaacccgt tacagtcaag atggaacacc aaagcgtcta tccttagcat
      781 tacaggattt aggatattca gggattaaat ttttagacca agagtcaaga aaaaaccctt
      841 atgaaaagca gtattttaat tatgttgttt ttgatgaaaa cataatatca atcaaggaca
      901 cctactacag caaacaacaa ggcgcaaccg aaggctttta ccttaacggt acagtccacc
      961 ttattgctga taacctaacc gatgaaacca ttatcccaac ttttgtacat gaattaggtg
     1021 gtcacaaagg ctttcaggag atgatgagtc caaaggcgta tgagctaatc atgcagcagt
     1081 ttgaccgctt agtagctcaa ggtaatgcca ttgctattaa agcca
//
LOCUS       DAOQMB010000812         1125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_119207_length_1125_cov_3.754206, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000812 DAOQMB010000000
VERSION     DAOQMB010000812.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119207_length_1125_cov_3.754206"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="PLY05_14145"
     CDS             complement(<1..359)
                     /locus_tag="PLY05_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNC04481.1"
                     /translation="MLNTPFSPWPSFTQEEAEAVSRVLLSNKVNYWTGQECRAFEKEF
                     ATWCGTTHAIALHNGTVALDLALIAAGIKAGDEVVVTPRTFLASASIVSVGAVPVFAD
                     VDADSQNITAQTIALVLT"
     gene            complement(368..1000)
                     /locus_tag="PLY05_14150"
     CDS             complement(368..1000)
                     /locus_tag="PLY05_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HNC04482.1"
                     /translation="MVKRLVVLGAGGHGAVVADAAECQGLWSEIIFLDDGWATKKNIY
                     SWPVLGALDKFASFINSETSFVVAIGNAQVRLEWLKKIQDLSGNIATIIHPSAVISRH
                     AQIGAGVVVFANAVVNIGAYIAVGCIINTGAGVDHDVKLAECVHICPNTHLAGNVKIG
                     SCSWIGIGTSVRQMIEIGSNVMVGAGSVIVSNVGNNMTVMGVPAKPKAFN"
     gene            complement(994..>1125)
                     /locus_tag="PLY05_14155"
     CDS             complement(994..>1125)
                     /locus_tag="PLY05_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005405344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HNC04483.1"
                     /translation="VDNQSLWLDIKILFLTVWKVVNRKDISADGEATMSKFTGSNKW"
BASE COUNT          366 a          273 c          175 g          311 t
ORIGIN      
        1 gttaaaacta gagctatggt ttgtgctgta atattttgag agtcagcatc aacatcggca
       61 aaaacaggca cggcaccaac tgaaacaata ctcgccgagg ccaaaaaagt gcgcggtgta
      121 acaacaactt catcacctgc cttaatgcct gcggcaatca atgccaaatc aagcgcgact
      181 gttccgttat gcaaggcaat cgcatgtgtt gtaccacacc atgtggcaaa ttctttttca
      241 aacgctctac attcttgacc tgtccaataa ttaactttat tggacaataa aacccgacta
      301 acagcttcag cttcttcttg ggtaaaactt ggccacggag aaaagggggt atttaacatg
      361 ataatcctta attaaaagct ttaggtttgg caggaacacc cattacagtc atattattgc
      421 caacattaga aacaatcacc gagccagcac ccaccataac attagaacct atttctatca
      481 tttgacgtac tgaagttcca atacctatcc aagagcaaga gccaatttta acattacctg
      541 caaggtgagt attagggcag atatgcacac attccgcaag cttgacatca tggtcaaccc
      601 ctgctcctgt attaataata caaccgactg caatataagc ccctatatta acaacagcat
      661 ttgcaaaaac aacaacgcca gccccaattt gtgcatggcg actaataaca gcagacggat
      721 gaataattgt tgctatatta cctgataaat cttgtatttt ttttagccac tctaagcgta
      781 cttgagcatt gccaatcgca acaacaaaag atgtttcact gtttataaag cttgcaaact
      841 tatctaacgc gcctaaaact ggccatgaat atatgttttt tttagttgcc catccatcat
      901 ctaaaaatat tatttctgac cataatcctt ggcattctgc ggcatcggca accactgcac
      961 catgcccacc tgctcctaaa acaaccaaac gcttcaccat ttattactac ctgtaaattt
     1021 tgacatcgta gcctcaccat cagcactaat atctttacga ttaacaactt tccaaaccgt
     1081 taaaaataat attttaatgt ctaaccataa cgattgattg tcaac
//
LOCUS       DAOQMB010000813         1122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_119817_length_1122_cov_10.708529, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000813 DAOQMB010000000
VERSION     DAOQMB010000813.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119817_length_1122_cov_10.708529"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLY05_14160"
     CDS             <1..283
                     /locus_tag="PLY05_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04484.1"
                     /translation="QLLESGVNYALDLSHLNILAVQTNHVEWSLVHELLSSPHCLEIH
                     LSDNHGYGDQHLPLSNPLALPWWWSLLSSANPNATIFSEGRQRVPIALA"
     gene            426..>1122
                     /locus_tag="PLY05_14165"
     CDS             426..>1122
                     /locus_tag="PLY05_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNC04485.1"
                     /translation="MEYQHTEQSPASIHAGNSLSLTVFSEQSTKAQTHTANAIRLIST
                     KGLSRSEWLAVRKQGIGGSDAAAAVGLNPYQSQLELWMIKTGRFTEDNGDTPDVNNHS
                     SVIPDIDSKMYWGQVLEPIVAQHYTKVTGRKVRKVNAILQHADSDKSWMLANIDYRVV
                     GSDEVQLLECKTAGEYGAKLWKDGVPEYVQCQVQHQLAVTGLQAADVCVLICGQQLKI
                     YRIERDEELIAKLI"
BASE COUNT          307 a          232 c          254 g          329 t
ORIGIN      
        1 acaactgctc gaatcggggg tgaattacgc cttagatttg tcgcatttga atattctagc
       61 agtacaaacg aatcatgtgg aatggagctt agtgcatgaa ttactgagta gtccccactg
      121 tttagaaatt catctcagcg acaatcatgg ttatggcgac caacatttac ccctgagtaa
      181 ccctcttgct ttgccgtggt ggtggtcatt actcagttcg gctaatccta atgcaacgat
      241 tttttcagaa ggcaggcaac gtgtgccgat agcattggct taatactttc gcggtgattg
      301 gctgaaatat tctttttacg ctatcaacaa tcagcttctt atcatccctc attcaacaca
      361 attattttca ttcacccaag cacagtctgt catgcaggct gtgctttttt tatgtcgagg
      421 tttttatgga atatcaacat actgaacaat cgcctgcctc tattcatgca ggcaactctc
      481 tgtctttaac cgtatttagt gagcaatcca ctaaagctca aacccacact gccaacgcga
      541 ttcgcctcat cagcaccaaa ggcttaagtc gtagtgaatg gttagctgtg cgtaaacaag
      601 gtattggcgg tagtgatgca gcagcggctg tcggtttaaa tccctatcaa tcccagttgg
      661 aattatggat gattaaaaca gggcgtttta ccgaagataa tggtgatacg cctgatgtta
      721 ataatcattc ttcggtgata cctgatatag actccaaaat gtattggggt caggttttag
      781 agccgattgt cgcccaacat tacaccaaag tcacaggccg taaagtgcgt aaggttaatg
      841 ccattctgca acatgccgat agtgacaagt catggatgtt ggccaatatt gattatcgag
      901 tcgttggtag tgatgaggtg caattactgg aatgtaaaac cgcaggcgaa tatggggcaa
      961 agctatggaa agatggcgtg cctgaatatg tgcaatgtca ggtacaacat caattagcgg
     1021 tcactggttt acaagccgcc gatgtctgtg tgttgatttg tggtcagcaa ctcaagattt
     1081 atcgcattga acgtgatgaa gagttaattg ccaaattgat tg
//
LOCUS       DAOQMB010000814         1121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_120047_length_1121_cov_6.212008, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000814 DAOQMB010000000
VERSION     DAOQMB010000814.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120047_length_1121_cov_6.212008"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLY05_14170"
     CDS             <1..146
                     /locus_tag="PLY05_14170"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836287.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HNC04486.1"
                     /translation="EVTPIREYDDRPIGTGVRGEMTAKLQQLFFDTVSGKNDTYKHWL
                     TFV"
     gene            383..>1121
                     /locus_tag="PLY05_14175"
     CDS             383..>1121
                     /locus_tag="PLY05_14175"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HNC04487.1"
                     /translation="MSPISPLIIWQFVDGKAGHEQQSSGLIKALEKFYPIQAIKIDIR
                     QHPVGVVDWLLKRLPDYPLPHYIIGAGHRTHLALLTAKRITKAKAIVLMKPSLPTSWF
                     DYALIPEHDSPTPHARVLVTQGVLNTIEPSTQQQTNKGLMLLGGASKRHGWDEISLVK
                     QMLLVLDATPHIQWQISTSRRTPEHTLLEVRNIAPHAKVWSANETPQGWIAEQLQQAS
                     QVWVTEDSISMIYESLTAGAQVGVFAVP"
BASE COUNT          337 a          234 c          228 g          322 t
ORIGIN      
        1 ccgaagtgac gccaattcgt gaatatgatg accgtccaat tggtacaggt gttcgtggtg
       61 aaatgacggc caaattacag cagttatttt ttgacaccgt gagtggtaaa aatgatactt
      121 acaaacattg gttaacgttt gtttaaaggt gtttgtctca accctctctc aaaggacaag
      181 gtatctacac aattattttg gagctaaaaa atctataaaa atgagcgtaa gtcccctctt
      241 gagaggggat ttagtggtgt gtcaaggcta aaaacagtgc ttttttgtct gttttaaccc
      301 acccctagcc cctccaagga ggggaatttc atcgttatag ttgtaatttt agcccttatt
      361 tatttttaag gcgtaggaaa tcatgtcacc aatctcccca ctcattattt ggcagtttgt
      421 tgatggcaaa gcagggcatg agcagcaaag ctctggctta attaaagcct tagaaaaatt
      481 ttatcccata caagccataa aaatagacat tcgtcagcac cctgttggtg ttgtggattg
      541 gctattaaaa cgcttacccg attatccttt gcctcattac attataggtg caggccatag
      601 aacccattta gcattattaa ccgctaaacg cattaccaaa gcaaaagcga ttgtgttaat
      661 gaaaccttct ttgcctactt cttggtttga ttatgcttta atccccgaac atgatagtcc
      721 cacacctcat gcaagagttt tagtcaccca aggggtatta aataccattg agccaagcac
      781 gcagcagcaa acaaacaaag ggttaatgtt attaggagga gcttctaagc gacatggttg
      841 ggacgaaatt tcgcttgtta aacaaatgtt attagtgtta gacgccactc ctcatattca
      901 atggcaaatt tcgacttctc gacgtacccc agagcatact ttattagaag tacgcaacat
      961 tgccccacac gccaaagtat ggtcagccaa tgaaacgcct caagggtgga ttgcagagca
     1021 attacaacaa gcatcgcaag tatgggtgac cgaagatagc atctcaatga tttatgaaag
     1081 tttaacagca ggtgctcaag ttggtgtatt cgctgtaccc a
//
LOCUS       DAOQMB010000815         1119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_120552_length_1119_cov_3.468985, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000815 DAOQMB010000000
VERSION     DAOQMB010000815.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1119
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120552_length_1119_cov_3.468985"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1086
                     /locus_tag="PLY05_14180"
     CDS             <1..1086
                     /locus_tag="PLY05_14180"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNC04488.1"
                     /translation="QEKLEAEQLKVLDEQTWNIIKDKTDIQLFRNYLKDFGNGQYVSD
                     AKKQIKKLEELLSNKKEEDYWQLIKEKTEAKVFADYLVEFPTGKYVPEAQALVAKYTV
                     VVGSTVVNIKNVRDCANCPDMVIVPKGSFLMGSTTDKTQHLVTINKPLAVAKYELTVG
                     EYNQCVKDNACYKAPKTGSQTDQHPVSGLRWQDAKNYINWLSNKAKRVYRLPTEAEWE
                     YFARANTKTTWSFGDDQATICQYGNIADETYNRVFSSGDTSCADNQAGSSSVGLFKPN
                     AFGLYDTIGNVYEWVEDCFVDNYQAAPTDGTAAFSKQCDKRVLRGGAYAVSAAKASSA
                     SRLAAEPDDAFYLNGVRLVRDVKEGEY"
BASE COUNT          376 a          168 c          249 g          326 t
ORIGIN      
        1 caagaaaaac ttgaggctga acagcttaaa gtattagatg agcaaacatg gaatatcatt
       61 aaagataaaa ccgatattca gttatttcgt aattatctca aagacttcgg taatgggcag
      121 tatgtttctg atgccaaaaa acaaatcaaa aaattagaag agttattaag caataaaaaa
      181 gaagaagact attggcaatt aattaaagaa aaaaccgaag ctaaagtttt tgcagattat
      241 ttagtagaat ttccaacagg taaatatgtg ccagaagcac aagcattagt tgcgaaatac
      301 acagtagttg ttggtagtac tgtggtgaat attaaaaatg ttagagattg tgcaaactgt
      361 cctgatatgg tgattgtacc taaaggtagc tttttaatgg gtagcactac cgataaaaca
      421 cagcatttag tcacaattaa taagccattg gcagttgcta aatatgaatt aacagtgggt
      481 gaatataatc aatgtgtaaa agataatgct tgttataagg ctcccaaaac tggaagccaa
      541 acagatcagc atccagtcag tggtttacgt tggcaagatg ccaaaaatta tattaactgg
      601 ttatctaata aggctaagcg cgtttatcgc ctacccacag aagcagaatg ggagtatttt
      661 gctcgggcta ataccaaaac aacatggagt tttggtgatg atcaagccac tatttgtcag
      721 tacggtaata ttgcagatga aacatataac cgcgttttct ctagtggtga tacgtcatgt
      781 gcagataatc aggcgggtag ctctagtgta ggtttattta aacctaacgc ctttggtttg
      841 tatgacacca ttggtaatgt ttatgagtgg gtagaagatt gttttgttga taactatcag
      901 gctgctccaa cagatggtac agcggcattc agcaaacaat gtgataaacg tgttttacgt
      961 ggtggtgctt atgccgtgtc ggcagctaaa gcgagttctg caagccgttt ggctgcagaa
     1021 cctgatgatg ctttttattt aaatggtgta cgtttggtac gtgatgttaa ggaaggtgag
     1081 tattaagcaa tctttggcaa aggcaagcta acagtaatt
//
LOCUS       DAOQMB010000816         1118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_120740_length_1118_cov_18.960489, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000816 DAOQMB010000000
VERSION     DAOQMB010000816.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120740_length_1118_cov_18.960489"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1118
                     /locus_tag="PLY05_14185"
     CDS             <1..>1118
                     /locus_tag="PLY05_14185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNC04489.1"
                     /translation="FFVSEKTHRYYYPNGILKTVSAKDNADRWFFRVRELNEPYEINI
                     DYDKANNNSLAIFMNFRVFNQQQQFLGVTGVGVSMERLYQLLGHYEQQYKKNIFLVDK
                     EGKIRLTGNNRLQDPRSIWQINGLKDYAAQLLSRQTNIIQYKTKDGKKYLVHVRYMPE
                     VRLYLFVESREDEAIAEVKQTLYINMIVLCFIVIATILLTNMTVGFYQRELKQLASLD
                     GLTQLLNRQAFEIVSERLLADSRRHQQSLSVMIADVDFFKRINDSYGHQTGDLVLKQV
                     ALALRESVRESDVVCRWGGEEFALFLPKCALDDAERIAEKIRQHIEELALHEAFYSVP
                     VTISIGVAEYQVTESLKDVFARADKALYRAKAGGRNQVV"
BASE COUNT          355 a          158 c          236 g          369 t
ORIGIN      
        1 ttttttgtct ccgaaaaaac acatcgctat tattatccaa atggaatttt aaaaacggta
       61 tctgccaaag ataatgcgga tcgttggttt tttagagtgc gtgaattaaa cgaaccctat
      121 gaaattaata ttgattatga caaagctaat aacaactctt tagcgatttt tatgaatttt
      181 agagtcttta atcaacaaca gcagttttta ggagtaacag gtgttggtgt gagcatggag
      241 cgtttatatc aattattagg ccactatgaa caacaatata aaaagaatat ttttttggtg
      301 gataaagaag ggaaaattcg tttaacaggc aataaccgtt tacaagaccc tcgatctatt
      361 tggcaaatta atggtttaaa ggattatgct gcacaacttt tatcacggca aacaaatatt
      421 attcaataca aaactaagga tggtaaaaaa tatttagtgc atgttcgtta tatgcccgaa
      481 gtacgtttat atttatttgt tgaaagccga gaggatgaag cgattgcaga ggtcaaacaa
      541 actctatata tcaatatgat tgttttgtgt tttattgtga ttgctacaat tttattaacc
      601 aatatgaccg ttggatttta tcaacgtgag ttaaaacaat tggctagttt ggatggttta
      661 acccagttat taaatagaca agcctttgaa atagtgagtg aaagactttt agccgatagc
      721 cgtcgtcatc aacaatcgtt aagtgtgatg attgccgatg tcgatttttt taaacgtatt
      781 aatgatagtt atggtcatca aacaggtgac ttggtattaa aacaagttgc tttagccttg
      841 cgtgaaagcg ttcgcgagtc ggatgtggtt tgtcgttggg gtggagagga gtttgcctta
      901 tttctaccca aatgtgcttt agatgatgca gagcgtattg ctgaaaaaat tcgtcaacat
      961 atcgaggaat tagctttaca tgaagctttt tattctgtac ctgtgactat tagtattggg
     1021 gttgctgaat atcaagtgac tgaaagccta aaagatgttt ttgcgcgtgc tgataaagcc
     1081 ttgtatagag ccaaagcagg gggtaggaat caagtcgt
//
LOCUS       DAOQMB010000817         1117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_120967_length_1117_cov_11.824859, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000817 DAOQMB010000000
VERSION     DAOQMB010000817.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120967_length_1117_cov_11.824859"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLY05_14190"
     CDS             <1..479
                     /locus_tag="PLY05_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04490.1"
                     /translation="KPKKSTAVLDSLRLPEKLTYSGCERIVFDLDKGTLNGLKATASQ
                     QEVKNALPCATGDTPDGESYNYGGGVFFLNHNFFMYTGNNFIEVRRDFKGSVTPNLLQ
                     QSQELVVARYGQPAFTRRGDYFYTMAYGCLQFTFNNGFVDEIDVHYQQCKAASEVR"
     gene            590..784
                     /locus_tag="PLY05_14195"
                     /pseudo
     CDS             590..784
                     /locus_tag="PLY05_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            781..>1117
                     /locus_tag="PLY05_14200"
     CDS             781..>1117
                     /locus_tag="PLY05_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04491.1"
                     /translation="MNPPINKNKSPANSTLPHFLGQSKTAHVGVVDSKASKQNILIPS
                     DVVNKFETGGKFSQSSTVGDRTITQVRDKLISRFGKRIIEQLESKGILKIHQSIDDNS
                     VPKEVRLSGI"
BASE COUNT          328 a          223 c          239 g          327 t
ORIGIN      
        1 taaaaccgaa aaaaagtaca gccgttttgg actctttgcg cttgcctgag aaattaacgt
       61 attcaggttg tgagcgcatt gtttttgatt tggataaagg tactttaaat ggtttaaagg
      121 caacggctag ccaacaagag gttaaaaacg cactcccttg tgcaacaggt gatacccctg
      181 atggcgaaag ttataactac ggtggcggtg tattcttttt aaatcataat ttttttatgt
      241 acacaggtaa taactttatt gaggtacggc gcgactttaa aggctctgtt accccaaatc
      301 tattgcagca aagccaagag cttgttgtgg ctcgttatgg tcaacctgcg tttactcgtc
      361 gtggtgatta tttttacacg atggcttatg gttgtttgca atttacgttt aataatggat
      421 ttgttgacga aattgatgtg cattatcaac agtgtaaagc agcgagtgag gtgcggtagc
      481 gtgacgactt atgcgattat cgcgtagcct gtatgcagcc tagcggaata cagtataaat
      541 ttattaccct gtgttacgtt gcacttcaca caggctacct agctctaatc aacgtgcgag
      601 aaacgaaaat acgaatggtt taattcgtca gtatttgcct aaatcgtctg cttttgatac
      661 tgtctcggac actcaaattc aagccattga agaggctctt aataatcgac ctagaaaatc
      721 actaggctgg tttactccaa gtcaggtcat ggctaatttt tatactgtcg cacttgctgc
      781 ttgaatccgc ctattaacaa aaacaaaagc cctgcaaatt ctacgcttcc ccacttctta
      841 ggacagtcaa aaactgccca tgtgggagta gtagatagca aggcttctaa gcaaaatata
      901 ctcataccat ccgatgttgt caataaattt gagacaggcg gcaagtttag ccaatcctca
      961 actgttggcg acagaacaat cacccaagta cgcgataagc ttattagccg ttttggtaag
     1021 cgcattattg agcaattgga aagcaagggt attcttaaaa tccaccaatc cattgatgac
     1081 aacagcgtac caaaagaggt gcgcttaagt ggaattg
//
LOCUS       DAOQMB010000818         1113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_121853_length_1113_cov_10.470699, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000818 DAOQMB010000000
VERSION     DAOQMB010000818.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121853_length_1113_cov_10.470699"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLY05_14205"
     CDS             <1..534
                     /locus_tag="PLY05_14205"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNC04492.1"
                     /translation="LSQVQHIDVAGISLKIEALKQSAQQLTLPPSKHNHIPQSQATNV
                     EQSAWEKGWLALQKLISIQHHNQTIAAPLLTQEQRVLVQQNVYLALQQAQMALWSHQT
                     QRYQQSLTNVEQLLMPYQQFNPHYAVFIKEIQLLKQQPMHELIVKLTNTPSVLAQLLT
                     QHTESEPSIPSQQDASL"
     gene            531..>1113
                     /locus_tag="PLY05_14210"
     CDS             531..>1113
                     /locus_tag="PLY05_14210"
                     /inference="COORDINATES: protein motif:HMM:NF018875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HNC04493.1"
                     /translation="MIKLAILLLFLLLGAWLGSVFLQDSGYVLIVWQQTSIELSLFLA
                     VLLLMLAAGLGLIALELLLGVFGLRALLNKWLQQRRFLRAQRSYHKGLEALTLEDYRR
                     AEKLFNQSAKISDEPLAAYLAAAQAAQGQQASQRAEDYLQLADSYQYRLIIQITRIRL
                     WLQTGQWEAAVAQLKSIYPHYKKEPVVNQLLLES"
BASE COUNT          372 a          197 c          208 g          336 t
ORIGIN      
        1 ttaagtcaag tacaacatat tgatgttgct ggtatttctc taaaaataga agccctaaaa
       61 cagtcagcac aacaattaac tttgccgcca agtaagcata atcatatccc acaatctcaa
      121 gcaacaaatg tagagcaaag tgcatgggaa aaaggttggc ttgctttaca gaagttaatt
      181 agtattcaac atcataatca aacgattgct gcgccactgc ttactcagga gcaacgggta
      241 ttagtacaac aaaatgttta tttggcatta cagcaagcac aaatggcttt gtggtcgcat
      301 caaactcaac gttatcaaca aagtttgact aatgttgagc agttattgat gccttatcag
      361 caatttaatc cacattacgc tgtttttatt aaagaaattc aactattaaa acaacagcct
      421 atgcatgagc taatcgttaa gctaacaaat accccgtcag tattggctca attattaacg
      481 caacatacag aatcagaacc aagtattcct agtcaacagg atgcaagttt atgattaaat
      541 tagccatttt gttgctgttt ttattgttag gcgcatggtt gggtagtgta tttttacaag
      601 acagtggata tgttttaatt gtttggcagc aaacttctat tgaattaagt ttatttttgg
      661 ctgttttatt attaatgttg gcagcaggat taggtttaat agctttagaa ttattattag
      721 gtgtatttgg tttacgcgca ttgctgaata aatggttgca acaaagacga tttttacgcg
      781 cacaacgtag ttatcacaaa ggtttagagg cattaacctt agaagattat cgtcgtgccg
      841 agaaattatt taatcaatcg gctaaaatca gtgatgaacc attagcggca tatttagcag
      901 ccgcacaagc ggcacaaggt caacaagcct cacaacgtgc agaagactat ttacagttag
      961 cagatagtta tcaatatagg ctcatcatcc aaataacccg aattcgatta tggttacaaa
     1021 ctggacaatg ggaagcggcg gtcgctcaac tcaaaagtat ttatccacat tacaaaaaag
     1081 agccggttgt taatcaatta ttattagaga gct
//
LOCUS       DAOQMB010000819         1112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_122067_length_1112_cov_14.156102, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000819 DAOQMB010000000
VERSION     DAOQMB010000819.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122067_length_1112_cov_14.156102"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1112
                     /locus_tag="PLY05_14215"
     CDS             <1..>1112
                     /locus_tag="PLY05_14215"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HNC04494.1"
                     /translation="AVTGCGKKEDAPKTDAATTTASSNGGMVVKIGHASPLTGPQAHL
                     GKDNENGVRMAIEELNAKGVTIGGQKVQFELESEDDQADPRIATTVAQKFVDDKVSGI
                     IGHLNSGTTIPSSRIYNDAGLVQISPSATNPKYTQQGYKGAFRVMANDVQQGKVLGEF
                     AVKDMAAKKIAIIDDRTAYGQGLADEFEKAAKAAGGNIVVHEFTNDKASDFTAILTKI
                     KGVAPDLVFYGGMDGQGAPMVKQMQTLGLKAQFLGGDGIHTAEFMKLAGAAAEGVTAS
                     LPGVPLEQMPHGADFKQKFEGKYGKIQLYAPYCYDAMMVMAEAMQRANSTEPAKYLPE
                     MAKTNYNGVTANIKFDDKGDLQGGSITVYRVAKGEW"
BASE COUNT          338 a          228 c          274 g          272 t
ORIGIN      
        1 gcagtcacag gttgtggcaa aaaagaagat gcgcctaaaa cagacgcagc aaccaccaca
       61 gcaagcagta atggcgggat ggtagtaaaa attggtcatg cttcaccctt aacaggccca
      121 caagcacatt tgggtaaaga taacgaaaat ggcgtgcgta tggccattga agaattaaat
      181 gctaaaggtg tgaccatcgg cggccaaaaa gttcagtttg agttagagtc cgaagacgac
      241 caagccgatc cgcgtattgc tactaccgtg gcgcaaaaat ttgttgatga taaagtcagt
      301 ggcattattg gtcacttaaa ctctggcaca acgattcctt cttcacgcat ttataacgat
      361 gctggtttag tgcaaatttc gccatccgca accaatccaa aatacaccca acaaggttat
      421 aaaggtgcgt tccgcgtcat ggccaatgat gtgcaacagg gtaaggtatt aggtgagttt
      481 gcggttaaag atatggcagc caaaaaaatc gccattattg atgaccgtac tgcttatggc
      541 caaggtttag ccgatgagtt tgaaaaagcg gctaaagctg ctggtggtaa tattgtcgtt
      601 cacgaattta ccaatgacaa agcttctgac tttaccgcga tcttaaccaa aattaagggc
      661 gttgcacctg atttagtgtt ttatggtggt atggatggtc aaggcgcgcc aatggtaaaa
      721 caaatgcaaa ccttgggctt aaaagcacaa ttcttgggtg gtgatggtat tcataccgcc
      781 gaatttatga agttggcagg tgctgctgcc gaaggtgtaa ccgcttcttt acccggtgtg
      841 ccgttagagc aaatgccaca tggcgcagac tttaaacaaa agtttgaagg caaatacggc
      901 aaaatccagt tgtacgcacc gtattgttat gatgcgatga tggtgatggc cgaagccatg
      961 caacgtgcaa attctactga gccagccaag tatttacccg aaatggcaaa aaccaattac
     1021 aacggggtga cggccaatat taaatttgac gacaaaggcg atttacaagg cggttcaatt
     1081 accgtctatc gtgtggccaa aggtgaatgg ca
//
LOCUS       DAOQMB010000820         1109 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_122779_length_1109_cov_22.671727, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000820 DAOQMB010000000
VERSION     DAOQMB010000820.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122779_length_1109_cov_22.671727"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            42..344
                     /locus_tag="PLY05_14220"
     CDS             42..344
                     /locus_tag="PLY05_14220"
                     /inference="COORDINATES: protein motif:HMM:NF027409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HNC04495.1"
                     /translation="MPKEPELSFFQQLLAFATLAGLGGVVNYLTTIKAGKKFKWSSFW
                     IELVCSAFVGVVIGLFFISTHMPIYMALAGAGVAGHLGTRSLMMLRDKYGLKDYER"
     gene            582..986
                     /locus_tag="PLY05_14225"
     CDS             582..986
                     /locus_tag="PLY05_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04496.1"
                     /translation="MSKPLHQPTDKTRAEIVALRSYGVPIKEVAAYIGIDDKTLYKYY
                     KDELDNSAIKANANVGKFLYQAASGQALATGATYSDCVRAAMFWAKTRMGWKETNVQE
                     HTGANGDPISLLITQIQGNSLGVSQSTANDYD"
BASE COUNT          327 a          194 c          256 g          332 t
ORIGIN      
        1 cctggtcgct atttttaaga cagagtctgt gaaaaaataa aatgccgaaa gagccagaat
       61 tgtcattttt tcagcaatta ttagcttttg ctactttggc aggcttaggc ggtgttgtta
      121 attatttaac gacaattaag gctggtaaaa aattcaagtg gtcgtcattt tggattgagt
      181 tagtttgcag tgcatttgtt ggcgtggtta ttggcttatt ttttatttca acacatatgc
      241 ctatttatat ggcgttggca ggggcaggag tagcgggtca tttaggcact cggtcgctga
      301 tgatgcttag agataaatac gggttaaaag attatgaaag ataagcctaa ttattttatg
      361 atttatacta taatcgcttt tatcagcttt ccgtttttgt tgttgtgggt tttttttaag
      421 gtgttgtgat tatggatatt ccgaagtata aaagcattga cattgattta ttgatacctt
      481 atgcgcgtac aaagcaagct aacttgagtg accaacttga gcgcggcggt gtgactccga
      541 cctgtacgct ccaaaacgcc ctaatcatta gggagtgcta aatgtcaaaa ccactgcacc
      601 aaccaaccga taaaacacgc gctgaaatag ttgccttgcg ctcttacggt gtacctatta
      661 aagaggtggc ggcatatatc ggtattgatg ataaaacgct gtataagtac tacaaagacg
      721 aattagacaa cagcgcaata aaagctaatg caaatgttgg taagttttta taccaagctg
      781 caagcggtca ggcgttggca actggcgcaa catatagcga ttgtgtaagg gcggccatgt
      841 tttgggctaa gacacgcatg ggttggaaag agactaacgt acaagaacac acaggcgcga
      901 atggcgaccc tatctcatta ctaataactc aaatacaagg caattcgtta ggggtcagtc
      961 aatcaacggc taacgattat gattgacccg tcatttgtac caaaaacacc cgaagaattt
     1021 aaaaagtgtt tgtctgaccc attgtggcgc atttgttcgg gtgctttgta caaaatcatt
     1081 atcaaaggcg atgatgataa agacggttt
//
LOCUS       DAOQMB010000821         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_123252_length_1107_cov_5.005703, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000821 DAOQMB010000000
VERSION     DAOQMB010000821.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123252_length_1107_cov_5.005703"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1107
                     /locus_tag="PLY05_14230"
     CDS             39..>1107
                     /locus_tag="PLY05_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNC04497.1"
                     /translation="MCNKADSQWHYALADQHQLMAGVVFDHLRAVPRTTDLSQPYDPN
                     LSVEQQHLYYLGTNNSLPVKIFQVTQDNLGVFLQDQYQWSSQLSSQIGIRFDDNSDYK
                     ASTSPYLGLKYNYTPETTFSLTYSQAYLAPSPSYSYEHFGSFTGQQDNQGRYLADTFR
                     VPNPDLQPETAKAVELIINQELNEHWQLISTAYGSHLQNLILFASPTANPVSEFINGG
                     WIKNTDHNVNLGESVAYGVELSSRYRQQHYELWGHYSFTDGHLSTNKGEEELPYNAKH
                     KLKLGATWRHKQGWFISPSLYFVGQNRVPSSASDGSRAVTVPSYQLFNMYAGYPQIVQ
                     GVDVFMCIENIASSKYYHAGGS"
BASE COUNT          327 a          228 c          223 g          329 t
ORIGIN      
        1 atctctttat tttacagaat ccctcaaatc atcgctattt gtgcaacaaa gccgatagtc
       61 agtggcacta cgcccttgcc gaccagcatc aactcatggc tggtgtggtg tttgatcatc
      121 tgcgtgccgt gccgcgaacc accgatttaa gccaaccgta tgacccgaat ctatcggtag
      181 aacaacaaca cctatattat ttgggcacaa ataacagttt accagtcaaa atttttcagg
      241 tgactcagga taatttaggc gtttttttac aagaccaata tcaatggtca tcgcagctta
      301 gtagtcaaat aggcatccgt tttgatgata attctgacta caaagccagt accagtcctt
      361 atttagggct taaatataat tatacgccag agacaacatt tagtttgact tatagccaag
      421 cctatttagc accatccccc tcatatagct atgagcactt tggtagcttt acaggccaac
      481 aggataatca aggtcgttat cttgccgata cctttagagt tcctaaccca gatttacaac
      541 cagaaacggc aaaagcagtg gagttgatca tcaatcaaga attaaacgaa cattggcaat
      601 tgattagtac tgcttatggt tctcacttac aaaatttgat tttatttgcc agtcctacgg
      661 ccaatcctgt cagtgagttt attaatggcg gttggattaa aaataccgac cacaatgtca
      721 atttggggga aagtgttgcc tatggtgtag agttaagtag tcgttatcgg cagcaacatt
      781 atgagctttg ggggcattat tcatttaccg atggtcactt aagcactaat aaaggcgaag
      841 aagagttacc ttataacgcc aaacacaaat taaaattggg cgcaacatgg cgacataaac
      901 aaggctggtt tattagcccc agtctgtatt ttgttggtca aaatcgtgtg ccaagttctg
      961 ccagtgatgg tagtcgggcg gtgaccgtgc cttcttatca attatttaat atgtatgcag
     1021 gttatcctca aatagtacaa ggcgttgatg tatttatgtg tatcgaaaat attgcctcta
     1081 gcaaatatta ccatgcaggt ggttcat
//
LOCUS       DAOQMB010000822         1107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_123297_length_1107_cov_3.391635, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000822 DAOQMB010000000
VERSION     DAOQMB010000822.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123297_length_1107_cov_3.391635"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PLY05_14235"
     CDS             <1..473
                     /locus_tag="PLY05_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04498.1"
                     /translation="VKLVRHGQLWLQLQATPAQAQQIRVGNAVQVAGCATGQVKSLAN
                     GLTANTQTRIIQVAIPQAQQCLQPLQYVSAQVLAQSSNSRGWTVPSQALVKQNNQWYV
                     FKQTAQGFEPIAVKVLSQSNKQTLIDGQALQDNMPIAITGLVQIKAAWSGMGGE"
     gene            477..>1107
                     /locus_tag="PLY05_14240"
     CDS             477..>1107
                     /locus_tag="PLY05_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNC04499.1"
                     /translation="MLNRLIAFALAQRVLIILLSVALLISGYFAFSKLPIDAFPDVSS
                     TQVQIIMKAAGMTPEEVESRIVAPIEQELLGIPHQSMLRSQSKYAIAVITLDFAEGTD
                     PYWARQQVNERLAGVMDVLPSNASGGIAPMTTPLGEMFMFTVEGDLSPMDKRQLLERV
                     IRPRLRGIEGVADVNSLGGYVKTFEVTPNLTALAARGVSLAQLQMAIEAN"
BASE COUNT          303 a          235 c          270 g          299 t
ORIGIN      
        1 tagtaaaatt ggtgcgtcat ggtcaattat ggttgcaatt acaagctacg ccagctcaag
       61 cccaacaaat tcgtgtcggt aatgcggtgc aagtagcagg ctgtgcgaca ggccaagtta
      121 aaagtttggc caatggttta actgccaata cccaaacccg tattattcaa gtggctattc
      181 ctcaagccca acagtgttta caacctttgc agtatgtcag cgcccaagtt ttagcacagt
      241 caagcaatag ccgaggttgg acagtgccta gccaagctct cgttaaacaa aataatcaat
      301 ggtatgtgtt taagcaaacg gcacaaggtt ttgagccaat cgccgtcaag gtattgagcc
      361 aaagcaataa gcaaacgctg attgatggcc aagctctaca agacaatatg ccgattgcta
      421 ttacaggttt ggtgcaaatt aaagccgcat ggtcgggcat gggtggggag taaaccatgt
      481 taaaccgttt gattgctttt gccttagcac aacgtgtgct gattatcttg ctcagtgtcg
      541 cgttgttaat ttcgggttat tttgccttta gtaaattgcc aattgatgct tttcctgatg
      601 tctccagcac ccaagtgcaa attattatga aagccgcagg catgacaccc gaagaagtgg
      661 aaagtcgtat tgttgctccc attgaacaag agttattagg cattcctcat caaagcatgt
      721 tgcgctcaca atcaaaatac gccattgcag tgattacctt agattttgcc gaaggtacag
      781 acccttattg ggcgcgacaa caagtcaatg agcgtttagc aggggtaatg gatgttttac
      841 ccagcaatgc cagtggcggt attgcgccaa tgaccacacc gttgggcgaa atgtttatgt
      901 ttaccgttga aggtgattta tcgccaatgg ataaacgcca attattagag cgcgtgatta
      961 gacctcgctt gcgtggcatt gagggagttg ccgatgttaa tagcttgggc ggttatgtga
     1021 aaacctttga ggtgacaccg aatctcaccg ctctagcggc gcgtggtgtg agcttggcac
     1081 aattacaaat ggcgattgaa gctaatc
//
LOCUS       DAOQMB010000823         1105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_123710_length_1105_cov_6.676190, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000823 DAOQMB010000000
VERSION     DAOQMB010000823.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123710_length_1105_cov_6.676190"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..704)
                     /locus_tag="PLY05_14245"
     CDS             complement(174..704)
                     /locus_tag="PLY05_14245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04500.1"
                     /translation="MDNKQFIELDKALHDRDSFDCGEAELNLFIQQFAAKHMQAGISR
                     TRVLVNKQKLANDKYSICAFYTVAPSSICRETLPQQLAKKLPHYPVPVFLLAQLAVDK
                     HYQGLGLGKITLIQALEYLWRVNQHLTAYAVVVDCLNQSAQDFYHQFGFQTLDKDHHK
                     TRLFLPMKTLNQLFSS"
     gene            complement(694..972)
                     /locus_tag="PLY05_14250"
     CDS             complement(694..972)
                     /locus_tag="PLY05_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019441776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNC04501.1"
                     /translation="MTTARLDLRIDDTIKSKAEKAAALLGLKSLTEYVVKLMDENASK
                     VIAEYQSITLKDDIFNQFMTACDHAQPPNDTLKKALQFSREQGFNNGQ"
BASE COUNT          335 a          162 c          237 g          371 t
ORIGIN      
        1 tcaaaagccg tatatagcag aatggtagta tatctatcga gtgttttacg aaaaacggtt
       61 ggattgtagg acgattttgc gtgttagata gggtgaaaat ttttatttga tgataatgag
      121 ctattgatta gtcctctgag ttgttggtag tttgacaaga ataatattgt gtattaactg
      181 ctaaataatt gattgagagt tttcatgggt aaaaacaatc gtgttttatg atgatcttta
      241 tctaaggttt gaaaaccaaa ttggtgataa aaatcttgag cggattgatt aaggcaatca
      301 accaccacag cataagcggt tagatgttga ttaacacgcc ataaatattc taaggcttgg
      361 attaaggtaa ttttgcctaa acctagccct tgataatgtt tatcaactgc aagttgtgcc
      421 aataaaaaaa cagggacagg ataatgaggc aattttttgg ctaactgttg gggtaaagtt
      481 tcacgacaaa tagagctagg tgcaaccgta taaaatgcac agatactgta tttatcatta
      541 gctaattttt gtttattgac taataccctt gtgcggctga tgcctgcctg catatgtttg
      601 gcagcaaatt gttgtataaa gagattgagt tctgcttcac cacagtcaaa agagtcgcga
      661 tcatgaaggg ctttatctaa ttcaataaat tgtttattgt ccattattaa agccttgctc
      721 acgactaaat tgtaaggctt tttttagcgt gtcattgggg ggctgagcat gatcacatgc
      781 tgtcataaat tggttaaaaa tatcgtcttt taaagtgata ctttgatatt cggcaatcac
      841 tttagacgca ttctcatcca tcaatttgac aacgtattcg gtcaggcttt ttaaacctag
      901 taaagcagcg gctttttcgg cttttgattt aatggtgtca tcaatgcgta aatctaaacg
      961 tgcagtggtc ataattggct cttttgtacg gaggttttcc gtacattata ggatggaaca
     1021 tgaatatagg caatctaagg atgtggtcac ttatggcttt gttgcacaaa tagcgatgat
     1081 ttgagggatt ctgtaaaata aagag
//
LOCUS       DAOQMB010000824         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_123912_length_1104_cov_12.420400, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000824 DAOQMB010000000
VERSION     DAOQMB010000824.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123912_length_1104_cov_12.420400"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLY05_14255"
     CDS             <1..368
                     /locus_tag="PLY05_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04502.1"
                     /translation="LGPEKLPHAARMAGAWLGRIRRTVIDIQAEIEKEVSAAELKQRM
                     NDEIARLKAATNPIEEEIKAVENTIHQQMTDASQTVDAAPAYLAQQTPLINHDEPQEN
                     NSVPTAPSLPPSKVNLIKS"
     gene            373..>1104
                     /gene="tatC"
                     /locus_tag="PLY05_14260"
     CDS             373..>1104
                     /gene="tatC"
                     /locus_tag="PLY05_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNC04503.1"
                     /translation="MSDLDNKQDEMPLVAHLIELRTRLMHMIGATLLVFFVLVAFRND
                     LYTLLAAPLRQLLPVGSSMVATDITSSFLAPFKLTFFVSFFIAIPYVLYQAWSFIAPA
                     LYKHEKKLAIPLLVSSVILFYIGISFAYFVTLGPVLKFFVSVAPENVDFMTDINAYLD
                     FCIKFFLVFGFTFEIPVVVLILIMAGIISTESLAEKRRYIIVICFAISMFVTPPDALS
                     MTVLAVLMWMLFEVGLFFGRFLEKKE"
BASE COUNT          287 a          208 c          221 g          388 t
ORIGIN      
        1 tgcttggccc cgaaaagttg cctcatgctg cacggatggc aggagcatgg cttggccgta
       61 ttcgccgtac ggtgattgat attcaagcgg aaattgaaaa ggaagtctcg gcagccgaac
      121 ttaaacaacg catgaacgat gaaattgctc ggctaaaagc agcaactaac cctattgaag
      181 aagaaatcaa agcggttgaa aacaccattc atcaacaaat gactgatgct tcacaaactg
      241 ttgatgctgc gcctgcctat ttggcacaac aaacgcccct catcaatcat gatgagcctc
      301 aagaaaataa ttctgtacca actgcaccct ctttgccacc tagcaaagtt aacttaataa
      361 aatcctaaga ctatgagtga tttagacaac aaacaagatg aaatgcctct tgtggcgcat
      421 ttaattgaac tacgcactcg tttgatgcac atgattggtg caacattgtt agtttttttt
      481 gtattagtgg cttttagaaa cgatttatat actttgctcg ctgctccact acgccaatta
      541 ttacctgttg gcagtagcat ggtggctacc gatattacgt cttctttttt agctcccttt
      601 aaactgactt tttttgtgtc attttttatt gctattcctt atgtgcttta tcaagcatgg
      661 tcttttattg cgcctgcttt atacaaacat gagaaaaaac tagccattcc cctactcgtt
      721 tctagtgtta tcttatttta tattggtatt tcgtttgcgt attttgtgac tttagggcca
      781 gtgttaaagt tttttgtcag tgttgccccc gaaaatgtcg attttatgac cgacattaat
      841 gcttatttag atttttgtat taagttcttt ttggtgtttg gttttacttt tgaaattcct
      901 gttgtggtat taattttgat tatggcaggc attattagca ccgaaagctt agccgaaaaa
      961 cgccgttata ttattgttat ttgttttgca atttcgatgt ttgttactcc acccgatgcg
     1021 ctgtcgatga cggtgcttgc tgtattaatg tggatgttgt ttgaagtggg tttgtttttt
     1081 ggtagatttt tggagaagaa agaa
//
LOCUS       DAOQMB010000825         1104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_123920_length_1104_cov_5.787417, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000825 DAOQMB010000000
VERSION     DAOQMB010000825.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123920_length_1104_cov_5.787417"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="PLY05_14265"
     CDS             <1..92
                     /locus_tag="PLY05_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNC04504.1"
                     /translation="MVIGAGERADDGVFWPQVRFDRKLFVHEV"
     gene            complement(405..1025)
                     /locus_tag="PLY05_14270"
     CDS             complement(405..1025)
                     /locus_tag="PLY05_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HNC04505.1"
                     /translation="MNRYIKQSILMMFLTLLGMQAMAIEEAKYTVLKQEGQLELRQYA
                     PHIVAETLVESDFEGAGNKAFRRLFNYISGNNESRQEIAMTAPVSQAEKGEKIAMTVP
                     VGQQLSGKNWVVSCMMPASYTLDTLPKPKDNQVILRQVPEHYVAAIRYSGFWSQKSYN
                     HHKQQLEAWIKQQSFKTTGEAIWARYNAPFTPWFFRRNEILVPVEQ"
BASE COUNT          306 a          213 c          217 g          368 t
ORIGIN      
        1 ttatggtgat tggtgcaggt gaacgtgcgg atgatggcgt tttttggcca caagtaaggt
       61 ttgatagaaa attatttgtc catgaggttt aaaaaatagc cattagatgt tttcttgcta
      121 tggagcttct tttatcagta aaagagtcgc acattagatg tattgtttta agttattggg
      181 tgagcatgtt atgtctagag acattaatcg tcaggtcaca gggctacaag tacgtgtgct
      241 atttaaaacc gtttcactca tctgggtacg ccagtgacta aatgtttctt ttaacttatt
      301 tgggggtaag gagtgcttat tgctaaatac taatttgtgc aacaaagctc tgttagtagc
      361 ccatacacaa cgcagcgaaa tatgagcttt atctttcaaa caggctattg ttctacaggc
      421 actaaaattt cattacgtct aaaaaaccac ggtgtaaaag gtgcattata tctagcccaa
      481 atggcttcac ctgttgtctt aaaagattgt tgtttaatcc acgcctcaag ttgttgttta
      541 tgatgattat agcttttttg actccaaaag cccgagtaac gaattgctgc gacataatgc
      601 tcaggcactt gtcttaaaat aacttggtta tctttaggtt tgggtagcgt gtctaaggtg
      661 taggaagctg gcatcataca actaactacc caatttttgc ctgatagttg ttgaccaaca
      721 gggacagtca tcgcaatttt ttcacctttt tccgcttgcg aaacaggtgc tgtcatggca
      781 atttcttgac gtgattcatt attgccagaa atatagttaa ataagcgtct aaaggcttta
      841 ttccctgcgc cttcaaaatc actttctacc agtgtttcag ccacaatatg aggcgcatac
      901 tgacgcaact ccaactgtcc ttcttgtttt aaaactgtgt atttggcttc ttcaatcgcc
      961 attgcttgca ttcctagcag agttaaaaac atcattaaaa tcgactgttt gatatagcgg
     1021 ttcatgatgc aatcactcct atttaatccc gactaccatg caccaaatct tccactctaa
     1081 taggaaagtg gcctaatttt cggt
//
LOCUS       DAOQMB010000826         1103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_124131_length_1103_cov_18.537214, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000826 DAOQMB010000000
VERSION     DAOQMB010000826.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124131_length_1103_cov_18.537214"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="PLY05_14275"
     CDS             complement(<1..110)
                     /locus_tag="PLY05_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNC04506.1"
                     /translation="MFDVGFSELILLGIVALVVLGPEKLPHAARMAGAWLG"
     gene            complement(110..358)
                     /gene="tatA"
                     /locus_tag="PLY05_14280"
     CDS             complement(110..358)
                     /gene="tatA"
                     /locus_tag="PLY05_14280"
                     /inference="COORDINATES: protein motif:HMM:NF002813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HNC04507.1"
                     /translation="MAGLSISHLLIMLVVVVLLFGTSKLKNLGKDLGGAIKGFKDSMK
                     DGEQAAANPQTPPPAQISQNAPIDSTATPVNTVKKDTV"
     gene            complement(370..711)
                     /locus_tag="PLY05_14285"
     CDS             complement(370..711)
                     /locus_tag="PLY05_14285"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011536070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HNC04508.1"
                     /translation="MTTDILAELDKILAERKNATAESSYVASLYHKGLNKILEKVGEE
                     ATETIIAAKDAGLSGKTDDLIYETADLWFHTLVMLGHFNIPSQAIIDELARRLHISGH
                     AEKAARQHTTK"
     gene            complement(701..>1103)
                     /gene="hisI"
                     /locus_tag="PLY05_14290"
     CDS             complement(701..>1103)
                     /gene="hisI"
                     /locus_tag="PLY05_14290"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNC04509.1"
                     /translation="MSFLETVKFDEQGLIPAIAQDAQTGRVLMVAWMNKEALQLTADT
                     KRGVYWSRSRKKLWHKGEESGHIQVVKEIQLDCDADVIILQIEQLGGIACHTGRQSCF
                     YRTLDDNQQWQIVEPVLKDPNAIYEHPKHDH"
BASE COUNT          300 a          235 c          225 g          343 t
ORIGIN      
        1 ccaagccatg ctcctgccat ccgtgcagca tgaggcaact tttcggggcc aagcacaaca
       61 agggcaacaa tgcccaataa aattaactcg ctaaaaccaa catcaaacat tagacagtat
      121 cttttttgac agtgttaaca ggcgttgctg tgctatcaat tggcgcgttt tggctaattt
      181 gagcaggtgg cggtgtttgt gggttggcag cggcttgttc gccatctttc atggagtctt
      241 tgaagccttt aatcgcacca cctaagtctt tgcctaagtt tttgagtttg gatgtgccga
      301 acaataacac gactacaact aacataatca ataagtgaga gattgataaa ccggccataa
      361 tagtacctct tatttggtcg tgtgttgacg agctgctttt tcagcgtgtc ccgaaatatg
      421 caagcgacga gccaattcgt caataattgc ttgggacgga atattaaaat gccccaacat
      481 gacaagcgta tgaaaccata aatcggctgt ttcataaatt aaatcgtcag ttttacctga
      541 taagcctgca tctttggccg caataatggt ttctgtcgct tcttcgccaa ctttttctaa
      601 aattttgtta aggcctttat gataaagact agccacatat gaagattctg ctgtagcatt
      661 tttgcgctcg gctaaaattt tatcaagttc ggctaaaata tcagtggtca tgctttgggt
      721 gctcgtaaat ggcattaggg tcttttaaca caggttcaac aatttgccat tgttgattat
      781 cgtctagtgt acgataaaaa catgattggc gtcctgtgtg gcaggcgatt ccgcctaatt
      841 gttcaatttg tagaatgata acatcagcat cacaatctaa ttgtatttct ttgacaactt
      901 gaatgtgacc agactcttca cctttatgcc aaagtttttt gcgtgagcgt gaccaataaa
      961 caccacgttt ggtgtctgcg gttaattgca aggcttcttt gttcatccat gcgaccatta
     1021 acacccgacc tgtttgtgca tcttgggcaa tggcaggaat caaaccttgc tcatcaaatt
     1081 ttactgtctc taaaaaactc ata
//
LOCUS       DAOQMB010000827         1095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_126045_length_1095_cov_5.684615, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000827 DAOQMB010000000
VERSION     DAOQMB010000827.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126045_length_1095_cov_5.684615"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1095
                     /locus_tag="PLY05_14295"
     CDS             <1..>1095
                     /locus_tag="PLY05_14295"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNC04510.1"
                     /translation="FNTEVTGAHFDESTGLWTVNTGTGHTVVARHFVLASGPLHVPAI
                     PKIKGIENFKGKVFHSAQWDHNYDLTGKNVVSIGTGGSAIQYVPEIAPQVKQLYVFQR
                     TPAWVIPRDERTYWGIEKAVFAKLPMLRKLHRARLYWSNESRVWPIFNPAIAEGLQKL
                     AEMFIKIQVKDKALAKKLTPDYTIGCKRVLISNKYYPTFNRKNVELVTDGILEVKENS
                     IVTKDGVTRPADCIILGTGFIVDPRIYMKDFPCTGLTGHNLVDDWKDGAEAYYGISVT
                     GYPNMYQLVGPNTALGHNSIIFMIEAQVNYILDCMKTLKAQGADYANIKASVQKSFNE
                     KVQANMQGTVWSSGCASWYQQDNGKNFTIWP"
BASE COUNT          331 a          219 c          238 g          307 t
ORIGIN      
        1 tttaacaccg aagtaacagg tgctcatttt gatgaaagca cagggttatg gacggtgaat
       61 acaggtactg gccacaccgt cgtggcgcgt cattttgtct tagccagtgg gccattgcac
      121 gtgcctgcta ttcccaaaat caaaggcatc gaaaatttta aaggcaaggt tttccattca
      181 gcacaatggg atcacaacta tgatttaaca ggcaaaaatg tggtgtcgat tggtacgggt
      241 ggtagtgcca ttcaatatgt tcccgaaatt gcgccacaag tcaaacaact gtatgttttt
      301 caacgaacac ctgcatgggt cattccacgt gatgaacgca cttattgggg tattgaaaaa
      361 gctgtttttg ccaaactgcc gatgttacgc aaattacacc gcgcacgttt atattggagc
      421 aacgaatcgc gtgtttggcc aatttttaat cctgccattg ctgaaggctt acaaaaactg
      481 gctgagatgt ttattaaaat acaagttaaa gacaaagcct tagccaaaaa acttactcca
      541 gactacacca ttggctgtaa gcgcgtgttg atttctaata aatactatcc gacctttaac
      601 cgtaaaaatg ttgagttggt cactgatggt attttagagg taaaagaaaa tagcattgtc
      661 accaaagacg gggtgactcg ccctgccgat tgtattattt tggggacagg ctttattgtt
      721 gatccacgaa tttatatgaa agacttccct tgtacaggtc taacaggtca taacttagtg
      781 gatgattgga aagacggtgc agaagcctat tatggcatca gtgtcacagg ttatcctaat
      841 atgtatcaac tcgttggccc taataccgct ttaggccaca actcaattat ttttatgatt
      901 gaagcacagg tgaattacat tttagattgt atgaaaaccc taaaagccca aggtgctgac
      961 tacgccaata tcaaagcttc ggtacaaaaa tcgtttaacg aaaaagtcca agccaatatg
     1021 caaggcacgg tctggagttc gggttgtgcc agttggtatc aacaagataa tggtaaaaac
     1081 tttaccattt ggccg
//
LOCUS       DAOQMB010000828         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_126465_length_1093_cov_9.711946, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000828 DAOQMB010000000
VERSION     DAOQMB010000828.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126465_length_1093_cov_9.711946"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLY05_14300"
     CDS             <1..383
                     /locus_tag="PLY05_14300"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005402062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNC04511.1"
                     /translation="RLLVTGLNPHAGENGYLGREEIDIINPTLTELRAEGFNVSDALP
                     ADTLFTSENLAQCDAVLAMYHDQGLPVLKAKGFGEAVNITLGLPIIRTSVDHGTALNL
                     AGTGQAKVGSLLAALKMAAEMAYD"
     gene            complement(525..>1093)
                     /locus_tag="PLY05_14305"
     CDS             complement(525..>1093)
                     /locus_tag="PLY05_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC04512.1"
                     /translation="VLLDEVEKAHPDVFNILLQVLEDGRLTDGQGRTVDFKNTVIIMT
                     SNLGSEVIRHLSEDKQYDQIKLAVLEVVGKHFRPEFINRIDEMVVFHPLQESQIRGIA
                     EIQLDRLRSRLAERDLKLELSEAAMEQMVVAGYDPVYGARPLKRAIQTGLENPLAQRL
                     LAGEFVSGDTILVDVEHSQLVFHKQRLH"
BASE COUNT          327 a          243 c          196 g          327 t
ORIGIN      
        1 cacgattatt agtcacaggg ttaaatcctc atgcgggtga aaatggttat ttaggccgtg
       61 aagaaattga cattattaat ccaaccttaa cagaattacg cgcagaaggt tttaatgtct
      121 cggatgcttt acctgctgat actttattta cctccgaaaa cttagcccaa tgtgatgctg
      181 tgttagcgat gtatcacgat caaggcttgc ctgtactaaa agccaaaggc tttggcgagg
      241 cagtgaatat cactttaggt ttaccgatta ttcgtacttc tgtcgatcat ggcacagcct
      301 taaacttagc tggcacaggc caagccaagg taggtagttt attagccgcc ttaaaaatgg
      361 ccgctgaaat ggcttatgat taatccctaa actaaccaca ccaagatata tctttttaaa
      421 caagatatat cgttagtgtt agccgtcttt ttataaactt gacagatttc ctattaggag
      481 agagctaggg tgaggtctaa ttttagccct ctacccataa ctatttaatg caatcgctgt
      541 ttatgaaaca ctaattggct atgttcaaca tctaccaaaa tcgtatcacc agacacaaac
      601 tcgcctgcta acaaacgttg ggctaaggga ttttctaagc cagtttgaat ggcacgtttt
      661 aacggccgtg cgccatacac agggtcataa cctgcaacca ccatttgttc catcgcagct
      721 tcgcttaatt ctaattttag gtcacgctca gctaaacgtg aacgtaatct atctaattga
      781 atttcagcaa taccacgaat ttgactttcc tgcaaaggat gaaacaccac catctcatca
      841 atacgattaa taaattcagg gcggaaatgt ttgccaacga cctccaatac cgctaattta
      901 atttggtcat attgtttgtc ttcacttaaa tggcgaatca cttctgagcc taagttagag
      961 gtcataataa tcacggtatt tttgaaatct accgtacgac cttgtccatc ggttaaacgc
     1021 ccatcttcta acacttgtaa taaaatatta aacacatcgg gatgggcttt ttccacttca
     1081 tccaacaaca cca
//
LOCUS       DAOQMB010000829         1093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_126471_length_1093_cov_7.915222, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000829 DAOQMB010000000
VERSION     DAOQMB010000829.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126471_length_1093_cov_7.915222"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="recA"
                     /locus_tag="PLY05_14310"
     CDS             <1..902
                     /gene="recA"
                     /locus_tag="PLY05_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNC04513.1"
                     /translation="GGLPKGRIVEIYGPESSGKTTLTLQVIAECQRQGGTAAFIDAEH
                     ALDPTYARKLGVDTDNLYVTQPDNGEQGLEIADMLVRSGAVDVIVIDSVAALTPKAEI
                     EGDMGDSHMGLQARLMSQALRKITGNAKRTNCLVIFINQLRMKIGVMFGSPETTTGGN
                     ALKFYASVRLDIRRTGAVKDGEEVVGSETKVKVVKNKVAPPFRETTFQIMYGQGTNRL
                     GELVDLGVDQNIIQKSGAWYAYQGNKIGQGKANACKYFEENPAVAEVIEAEVRNKLLS
                     IPAPNAASEQEAAEAALEGEDFA"
BASE COUNT          323 a          207 c          250 g          313 t
ORIGIN      
        1 ttggtggttt acctaaaggt cgtattgtcg aaatttatgg cccagagtca tcaggtaaaa
       61 caaccttaac cctacaagtg attgccgaat gccaacgtca aggtggcaca gctgcattta
      121 ttgatgccga acacgcactt gacccaactt atgcacgcaa attaggcgtt gataccgata
      181 acttatatgt cactcaacct gacaatggcg aacaaggact tgaaattgcc gatatgttag
      241 tacgttctgg ggcagttgat gtgattgtaa ttgactctgt ggccgcgctt acccctaaag
      301 ccgaaattga aggcgatatg ggtgattcgc acatgggttt acaagcacgt ttgatgagtc
      361 aagccttgcg taaaatcacg ggtaatgcca aacgtactaa ctgtttagtg atttttatta
      421 atcaattgcg tatgaaaatt ggtgtaatgt ttggtagccc cgaaaccaca acaggtggta
      481 atgcacttaa gttttatgct tctgtgcgtt tagatattcg tcgtacaggt gcggtgaaag
      541 atggtgaaga agttgtgggt tctgaaacca aagttaaagt ggtaaaaaac aaggttgctc
      601 caccattccg cgaaaccact ttccaaatta tgtatggtca aggtacaaac cgtttgggtg
      661 agttggttga tttgggtgtt gaccaaaata tcattcaaaa atcgggggct tggtatgctt
      721 atcaaggcaa caagattggc caaggcaaag ccaatgcttg taagtatttt gaagaaaatc
      781 cagccgttgc tgaggtgatt gaagccgaag tgcgtaataa attattgtct attcctgcac
      841 ctaatgcagc ctctgaacaa gaagctgctg aagctgcgtt ggaaggcgaa gattttgctt
      901 aattaaacaa aaaacaagtg taggctgggc attttgccca gcttattgtt tgctaaacca
      961 aagcctatat ccgtttagat actcagcata aaaccaataa actcaggagc tatctaaacc
     1021 ttcaaatgca aaccagaggc tcttttggtt tcttgttcta tggtttttag caaaattggc
     1081 tttaatgccc ata
//
LOCUS       DAOQMB010000830         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127176_length_1090_cov_19.540097, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000830 DAOQMB010000000
VERSION     DAOQMB010000830.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127176_length_1090_cov_19.540097"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..303
                     /gene="grxC"
                     /locus_tag="PLY05_14315"
     CDS             49..303
                     /gene="grxC"
                     /locus_tag="PLY05_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HNC04514.1"
                     /translation="MTAVTVYSTGTCPWCVQAENLLKRVGATVEKIRVDQSESQLESM
                     MTRSGRRTVPQIFVGDVHIGGFNDLVACEQSGELLKLINK"
     gene            423..1067
                     /locus_tag="PLY05_14320"
     CDS             423..1067
                     /locus_tag="PLY05_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNC04515.1"
                     /translation="MPRAFAELTFTPAVKSAQTRYGSREANQGFELATEPRNELTARE
                     IAFIAERNSFYQATVNEEGWPYVQHRGGARGFLKALDSRTLGYADLSGNRQYLSVGNL
                     TANNKISLFLMDYPNKRRLKLWGTTQIIHADDHPELLAQLEMPDYRAPVERGILIRVE
                     AWDWNCPKHITPRYSEDELTEQLADLHQQIAQLKVQLQDQQRALHSGGAELFKP"
BASE COUNT          299 a          232 c          251 g          308 t
ORIGIN      
        1 atttcagcga aatgcgtaag tccattttta actctgagga gaaccatcat gactgctgtc
       61 accgtctata gtacaggtac ttgcccttgg tgtgtgcaag ccgagaattt attaaaacgg
      121 gtgggtgcaa cggttgaaaa aatccgtgtt gatcaatcag agtctcaatt agagtctatg
      181 atgacacgta gtggccgtcg tactgtaccg caaatttttg ttggcgatgt tcatattggt
      241 ggctttaatg atttagttgc ttgtgaacag agtggcgaat tgctaaaact gattaacaag
      301 taaggactta ggcatttcac cacatttagt gcgttttgta cccccctcag tcccccctta
      361 ataagggggg atgccgaagg caggggggta accgcgttca ccccttggtt tggagaacta
      421 tcatgccgcg tgcttttgct gaattaactt ttacgccagc cgttaaatcg gcacaaacac
      481 gttacggcag tcgtgaggca aaccaaggct ttgaactggc gactgagcct cgtaacgaac
      541 tcactgcccg tgaaattgct tttattgccg aacgtaatag cttttatcag gcaaccgtta
      601 acgaagaagg ttggccgtat gtgcaacatc gtggtggtgc acgtggcttc ttaaaagcct
      661 tagacagtcg taccttgggt tatgccgatc tttctggaaa tcggcaatac ctgagtgttg
      721 gcaaccttac tgccaataat aaaatttcct tatttttgat ggattacccc aataaacgtc
      781 gcttaaaact ttggggaacg acacagatta ttcatgctga tgatcatccc gaattattag
      841 cacaattaga gatgcctgat taccgtgcac ctgttgaacg tggtatttta attcgtgttg
      901 aggcatggga ctggaattgt ccaaaacata ttacgccccg ttatagtgaa gatgaactta
      961 ctgaacaatt agcggactta catcaacaga ttgcacaact caaagtgcag ttacaagacc
     1021 agcagcgcgc attacactct ggtggtgctg aactatttaa accttaaaca aaataggact
     1081 gacgcatttc
//
LOCUS       DAOQMB010000831         1090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127179_length_1090_cov_10.749758, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000831 DAOQMB010000000
VERSION     DAOQMB010000831.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127179_length_1090_cov_10.749758"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="PLY05_14325"
     CDS             <1..140
                     /locus_tag="PLY05_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005240891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNC04516.1"
                     /translation="AGSFKSEGLGICLFEKMQGDDPTSLIGLPLMKLVTFLKNEDYQI
                     V"
     gene            221..985
                     /locus_tag="PLY05_14330"
     CDS             221..985
                     /locus_tag="PLY05_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HNC04517.1"
                     /translation="MSKKLFSTLLIGLFVSTNSYAHWQPTGKLSWQIQFAGTLNTNVN
                     AQVYDIDLFDTPQTTIQQLQNQGKKVICYMSAGSSENWRPDFNRFPSSVKGRNLSGWA
                     GEKWLDIRRLDILMPIMSDRMALAKQKGCDAIDADNVDGYSNRTGFKLTYNDQLNYNR
                     ALANEAHRLGLAIGLKNDLGQIVDLVNYFDFAINESCIIYNECSLVSPFIAQNKPVFH
                     IEYQNDINTICAVGTSYGFSSMKKNTNLDEWRESCL"
BASE COUNT          350 a          184 c          210 g          346 t
ORIGIN      
        1 gtgcaggtag ttttaaaagt gaaggtttag gcatttgttt atttgaaaaa atgcaaggtg
       61 atgatccaac aagtttgatt ggtttgccac ttatgaaact ggtcacattt ttaaaaaatg
      121 aggattatca aatagtttag tgtgccaatt ggcacacaga atcttatgtg gatatgttta
      181 tgatcgtgct aaatacatat caaccgacaa ggatagcatc atgtccaaaa agctttttag
      241 cacacttctt atcggattat tcgtttctac taatagttac gcacactggc aacccacagg
      301 caaactaagt tggcaaatcc aatttgctgg cacgttaaat accaacgtca atgctcaagt
      361 ctatgatatt gatttatttg acacacctca aacaacgatt caacaactac aaaaccaagg
      421 caaaaaagtt atttgttata tgagtgctgg tagctctgaa aattggcgac ctgattttaa
      481 tcgcttccct agctcggtta aaggccgcaa tttaagtggt tgggcaggtg aaaaatggct
      541 agatattcgt cgtttagata ttttaatgcc gattatgagt gatcgtatgg cactggccaa
      601 acaaaaaggt tgtgatgcaa ttgatgctga taatgtggat ggttatagca accgaacagg
      661 ctttaaactg acatataatg atcaattaaa ctataaccga gctttagcaa atgaagctca
      721 tcgcttgggt ttggcaattg gtttaaaaaa tgatttaggt cagattgttg atttagtaaa
      781 ctattttgat tttgcgatta atgaaagttg tataatttac aatgaatgca gtttagtatc
      841 tccatttatc gctcaaaata aacccgtttt tcatattgaa tatcaaaacg atatcaatac
      901 catttgtgct gttggcacta gctatgggtt tagctcaatg aaaaaaaata caaacttaga
      961 tgaatggcgg gaaagctgtc tatagtcatc aaaaaggggc attttctgcc cctttttatt
     1021 taccaaatat tattagattt gcctcgcttc tcggcaacat cgcgtttttg ctgacgcaaa
     1081 tagccaccac
//
LOCUS       DAOQMB010000832         1089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127579_length_1089_cov_2.164410, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000832 DAOQMB010000000
VERSION     DAOQMB010000832.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127579_length_1089_cov_2.164410"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1089)
                     /locus_tag="PLY05_14335"
     CDS             complement(<1..>1089)
                     /locus_tag="PLY05_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04518.1"
                     /translation="GQLKLGSKSDFSKNYDVISNTGSGKVEFYLSAEALLSLKKGGEY
                     DKDYEDEVCRSGSNATGRILLGTIPLAKVIGKIPGAQVVELTTDINIPVCLVVNAETS
                     MSYDLINLSGKLSSTIVFEGGQSPKINNIPSLTVTIPPSTAKLADDATVLSATGKKQL
                     KAEAEGEAGIEFGIEAGDKLGAFAAGASVAVLGKLSLTGTFGGEAISKNFSSLDTEPT
                     VCLELKSEVEVRALGFTDSWLNKKPLTVVGTIPLGEPRTKKWGLCKAEYTKISATGKE
                     LPDSATEWNCVLDKQTGLMWEKKTDDDGLRDKDHRYSWFEDSSGYADTRDFYTPKNGY
                     NTVPSYVKPYGYDCNNTLAKCNTQAYAQ"
BASE COUNT          259 a          279 c          200 g          351 t
ORIGIN      
        1 cttgggcata ggcttgggtg ttgcacttag ccaaggtgtt gttgcaatca tagccatagg
       61 gttttacata actaggcaca gtgttgtagc cattttttgg cgtataaaaa tcacgggtgt
      121 cggcataacc actgctgtct tcaaaccaac tatagcggtg gtctttatcg cgtaaaccat
      181 catcgtctgt ttttttctcc cacattaagc ctgtttgttt atctaacaca caattccact
      241 cagtggcact gtcgggtagc tctttgcctg ttgcgcttat tttggtgtat tctgctttac
      301 acaaccccca ttttttagtg cgtggctcac caagtggtat cgtacccacc acggttaaag
      361 gctttttatt caaccaacta tctgtaaatc ccaaagcacg cacctcaacc tcacttttta
      421 actccaaaca aaccgtgggt tcggtgtcta aactgctaaa gtttttgcta atggcctcac
      481 cgccaaacgt acctgttaag ctcagtttac ccaacactgc caccgaggct cctgcggcaa
      541 atgcgcctag tttgtctcct gcttctatgc caaattctat gcctgcttcg ccttcagctt
      601 ctgctttaag ttgtttttta cctgttgcgc tcaatacggt agcatcgtct gccaatttgg
      661 cagtactcgg tggtatcgtt accgttaagc taggtatatt attaattttg gggctttggc
      721 caccctcaaa cacaatggta gaggacaatt tacccgacaa attaattaag tcataactca
      781 tgcttgtttc ggcattcacc accaaacaca caggtatatt aatatcggtg gtcaactcca
      841 ccacttgcgc accgggtatt ttaccaatga cctttgctaa aggaatggtg cctaacaaaa
      901 tacgtcctgt tgcattgctg ccactgcgac aaacctcatc ttcatagtct ttgtcatatt
      961 cgccgccttt ttttaggctc aacagtgcct ctgctgacaa ataaaactct actttgccac
     1021 tgcccgtatt gctaattaca tcgtagtttt tactaaaatc gctttttgag cctaatttta
     1081 actgtccct
//
LOCUS       DAOQMB010000833         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127928_length_1087_cov_19.885659, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000833 DAOQMB010000000
VERSION     DAOQMB010000833.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127928_length_1087_cov_19.885659"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLY05_14340"
     CDS             complement(<1..581)
                     /locus_tag="PLY05_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04519.1"
                     /translation="MSKLESSAEIQKIGQLLGVKGDDLSFLQDLPVKELINLRGLTTD
                     KLFNDGRKLFQKLASASKLMPAGITASIGEKVFGPMLCARIASEMPYQRAVELAQKMS
                     IPFLGKVTLELDPRRVKDIIANMPLDRMRAVAKELLAHRHYMIMGGFVGFMKPDNLKI
                     ILDDVANEEALLHIGFFIEQKHIVSEIMRMLPKT"
     gene            complement(583..810)
                     /locus_tag="PLY05_14345"
     CDS             complement(583..810)
                     /locus_tag="PLY05_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04520.1"
                     /translation="MSQQLALEKLAATLKTSANQIEGLNRLSEAQLNTLNQYIEQAQL
                     KQRETLNNAIEEGLSYVPALLRGAVKAIVRG"
BASE COUNT          319 a          223 c          191 g          354 t
ORIGIN      
        1 gttttgggta acatccgcat aatttcactg acaatatgtt tttgctcaat aaaaaaacca
       61 atatgtaata aggcttcttc attggctaca tcgtctaaaa tgatcttcag attatctggc
      121 ttcataaaac caacgaagcc gcccataatc atataatgac gatgtgctaa caactctttt
      181 gccacagcac gcattctgtc taaaggcata ttggcaataa tatctttaac acgacgcgga
      241 tcaagctcta aggtcacttt acccaaaaat ggaatactca ttttttgtgc caattcaacc
      301 gctctttggt aaggcatttc actggcaata cgtgcacaca acatcgggcc aaagactttt
      361 tcgccaatac tcgcggtaat gcccgcaggc attaacttgc tggctgaagc taatttttga
      421 aatagtttac ggccatcgtt aaagagtttg tctgtggtta aaccgcgtaa attaatgagc
      481 tctttgacag gaagatcctg taaaaagctt aaatcatcgc ctttaacacc taataactgc
      541 cctatttttt gaatttctgc gctgctttct aacttgctca tattaacctc tgacaatcgc
      601 ttttaccgca ccacgtaaca aggctggcac ataacttaag ccttcctcaa tcgcattgtt
      661 tagcgtttct ctttgtttga gttgtgcttg ttcaatatat tgatttaagg tgtttagttg
      721 tgcttcagac aaacgattta atccctctat ttgattggct gaggttttta aggtagcagc
      781 tagcttttct aaagcaagtt gttggctcat aacagatact ctatgggctg tttaggtttc
      841 gactacgctc aaccgacata aatgtaccct gagcgtagtt taagggtgat ggagttaact
      901 attctatcaa gctactatgc caagttaagt gacaaaaatc attgacttct ttgacaaata
      961 gaaaactttt aaatatcagt gtttagcact attgatactg gcttcactat ctttgcttaa
     1021 tttaaaaaca aattctaaat atttacgcac aggtgtggga aataataccg atgtgacttg
     1081 gccaata
//
LOCUS       DAOQMB010000834         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127929_length_1087_cov_19.600775, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000834 DAOQMB010000000
VERSION     DAOQMB010000834.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127929_length_1087_cov_19.600775"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1087
                     /locus_tag="PLY05_14350"
     CDS             <1..>1087
                     /locus_tag="PLY05_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC04521.1"
                     /translation="HSFAVCSDSDVGKKRKKDDDIVQTFAHELRYPATTDAKRLAQEM
                     LKRHDDDHMSVVFSTYHSINVISDAQNAYQLAEFDLVICDEAHRTTGATFEGDDESNF
                     VKVHDGEFIHAKKRLYMTATPRIYGEAAKASADKDNVTLCSMDNAALFGHELYTINFS
                     EAVKRELLCDYKVIVLAVDENHINARLQNLLKDEDNQLKVDDAAKIVGCWKALSKHGT
                     QEDLIGDPNPMRRAVAFCQVIEPNYKGSTHKVSSKNIANMFQAVVEAYQEQEADKSST
                     IDTYLQCHAEHIDGSMNASQKEEKLTWLKEETPENTCRILSNVRCLSEGVDVPALDAV
                     LFLTPRNSQVDVVQSVGRVMRKAEGKKR"
BASE COUNT          332 a          219 c          246 g          290 t
ORIGIN      
        1 tacatagctt tgcggtatgt agtgacagtg atgtaggcaa aaaacgtaaa aaagatgatg
       61 acattgtgca aacctttgcc catgagttac gttatccagc cactaccgat gccaaacgcc
      121 ttgcacaaga aatgcttaaa cgccatgacg atgaccacat gagcgttgtc tttagtacct
      181 atcactctat taacgtcatt agtgatgctc aaaacgctta tcaactggcc gagtttgatt
      241 tagtcatatg tgatgaagcc caccgcacca caggcgcaac ctttgagggt gacgatgaat
      301 ctaactttgt caaagtgcat gatggcgaat ttattcatgc aaaaaaacgc ttatacatga
      361 ctgcgacacc gcgtatttat ggtgaagccg ctaaagccag tgccgacaaa gataatgtta
      421 ctttatgctc aatggataac gctgctttgt ttggccatga gttatatacc attaacttct
      481 cagaagcggt taaacgtgag ttattgtgtg attacaaggt gattgtctta gcggttgacg
      541 aaaatcatat taatgctcga ttacaaaacc tattaaaaga tgaggataac caacttaaag
      601 tcgatgatgc cgccaaaatt gtcggctgtt ggaaagcctt gtctaaacac ggcacacaag
      661 aagatttaat cggcgaccca aacccgatgc gtcgtgctgt tgctttttgt caggtgattg
      721 aacctaacta caaaggcagt acccataaag ttagctctaa aaacattgct aatatgtttc
      781 aggcggtggt tgaggcgtat caagaacaag aagccgataa atcatcaacc attgatactt
      841 atttgcaatg tcatgccgaa catattgacg gcagtatgaa cgcctcacaa aaagaagaaa
      901 aactcacttg gctgaaagaa gaaacgcccg aaaatacctg tcgtattttg tctaatgtgc
      961 gttgtttgtc ggagggtgtg gatgtaccag cccttgatgc cgtgttgttt ttaacgccgc
     1021 gtaatagtca ggtggacgtg gtgcaatcgg ttggtcgtgt gatgcgtaaa gccgaaggca
     1081 aaaaacg
//
LOCUS       DAOQMB010000835         1087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_127941_length_1087_cov_5.131783, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000835 DAOQMB010000000
VERSION     DAOQMB010000835.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127941_length_1087_cov_5.131783"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..353)
                     /locus_tag="PLY05_14355"
     CDS             complement(99..353)
                     /locus_tag="PLY05_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04522.1"
                     /translation="MNFQQERDDYMSKQQQSLMKFDPATGEERPYPSHAAQWRDWHGH
                     GTAWLFNPWTGQRRDARLVGTDTTGHLIIPPDEPIYAADD"
     gene            complement(494..760)
                     /locus_tag="PLY05_14360"
     CDS             complement(494..760)
                     /locus_tag="PLY05_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04523.1"
                     /translation="MKKFLFVGGDLDGKFVETDERTEYVHQTTNFVKTEEWLEKNPPK
                     EIDVKTQVYKKSLFGNYQKSVVFYALEGMVFNNDLMRRIFDLTK"
BASE COUNT          277 a          211 c          200 g          399 t
ORIGIN      
        1 caccccagcc gattacggtg ttttttaaca ggtttttagc aaccttgctc attctaaaat
       61 tgttgtgggg tgttgcaggt tagcgcagac gttagatgtt aatcatcggc agcataaata
      121 ggctcatcag gcggaataat caaatgacct gttgtgtctg tccctaccaa tcgcgcatct
      181 cttcgctgtc ctgtccaagg attaaataac catgctgtac catgtccatg ccagtcgcgc
      241 cattgtgctg catggcttgg gtatggcctt tcttcgcctg ttgctgggtc aaatttcatt
      301 aaagattgtt gttgcttgct catataatca tctctctctt gttgaaaatt catcgccact
      361 cttaaacaca tctaactctc gctcaacaac gataacaacc cagccgataa ttgtgttatg
      421 tttgcagtta ttggcaacct tgcatttttt ggctttgttg tgggttgtta cgtgttagct
      481 ttttagttag atgttattta gttaaatcaa agattctacg cattaaatca ttattaaaca
      541 ccatgccttc aagcgcataa aaaacaacac ttttttgata attaccaaaa agtgattttt
      601 tataaacttg tgttttaaca tcaatttctt taggcggatt cttttctaac cattcttctg
      661 ttttgacaaa gtttgttgtt tggtgaacat actcagtgcg ctcatcagtt tcaacaaatt
      721 tcccatctaa atcaccgcca acaaataaaa actttttcat agtcatctcc aaattcatcg
      781 ttaatcattt acacatctaa ctcacggttc aagcgcgaaa acaacccaag cggtcacggc
      841 tgttttttat ttagtgattc agcttattag cctactttgt tgtggttgtt tcggcttaac
      901 cttggtagtt agaagttctt tggggtagat ggttttgttt tgatgctttc ggaagtgcaa
      961 agtatttcaa gtgcgaaaat ttgagttttt taagtaatca gcaactcagg tttttatact
     1021 tgagttgaaa tcgttattta aagcaatggt ttaacttctt gtgtgattgc ttttggcgtt
     1081 tgtgttt
//
LOCUS       DAOQMB010000836         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_128420_length_1085_cov_9.400000, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000836 DAOQMB010000000
VERSION     DAOQMB010000836.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128420_length_1085_cov_9.400000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1085
                     /gene="thiC"
                     /locus_tag="PLY05_14365"
     CDS             <1..>1085
                     /gene="thiC"
                     /locus_tag="PLY05_14365"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNC04524.1"
                     /translation="LRFGHLRQPRRALAGKNVTQMHYAKQGIITPEMEFVAIRENLKL
                     QEARANGLLNQHEGQSFGAAIPKEITPEFVRQEVALGRAIIPANINHPELEPMIIGRN
                     FLVKINGNIGNSALVSSIEEEVAKMTWAIRWGSDTIMDLSTGKNIHETREWILRNSPV
                     PIGTVPIYQALEKVDGIAENLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYIPMTAKR
                     LTGIVSRGGSIMAKWCLAHHKENFLYTHFDEICQIMKAYDVSFSLGDGLRPGSIQDAN
                     DEAQLGELKTLGELTQIAWQHDVQVMIEGPGHVPMHMIKENMDLQLKWCHEAPFYTLG
                     PLTTDIAPGYDHITSAIGAAMIGWYGTA"
BASE COUNT          313 a          215 c          248 g          309 t
ORIGIN      
        1 ttgcgttttg gtcatttacg ccagccgcgt cgtgcgttag caggtaaaaa cgtcacgcaa
       61 atgcactatg ccaaacaagg cattattacc cccgaaatgg aatttgtcgc cattcgtgaa
      121 aacttaaaat tacaagaagc tcgcgccaat ggattattaa atcagcatga aggtcaatcg
      181 tttggtgcgg cgattcctaa agaaattacc cccgaatttg tccgtcaaga agtggcttta
      241 ggtcgtgcga ttattcctgc caatattaac catcccgaat tagagcccat gattattggc
      301 cgtaacttct tggtaaaaat taacggtaat attggtaatt cagcccttgt ttcttccatc
      361 gaagaagaag tcgcgaaaat gacgtgggca attcgctggg gttctgacac gattatggat
      421 ttatcgacag gtaaaaatat ccacgaaacg cgtgaatgga ttttgcgtaa ttcacctgta
      481 ccgattggca cagtaccgat ttatcaagcc ttagaaaaag ttgacggtat tgccgaaaat
      541 ttaacgtggg aaatcttccg cgatacctta attgaacaag ccgagcaagg tgtggattat
      601 tttaccattc acgcaggcgt gttgttacgt tatatcccga tgaccgccaa acgtttaaca
      661 ggtattgtct cgcgtggtgg ttcgattatg gccaaatggt gtttggcaca tcataaagaa
      721 aactttttat atacccattt tgacgaaatt tgccaaatta tgaaggctta tgatgtgtct
      781 ttctctttgg gtgatggttt aagaccgggt tcaattcaag atgccaatga cgaagcgcaa
      841 ttaggtgaat taaaaacttt gggtgaatta acccaaattg cgtggcaaca tgatgtgcaa
      901 gtgatgattg aagggcctgg ccatgtgcca atgcacatga ttaaggaaaa tatggactta
      961 caattaaaat ggtgtcacga agcaccattt tatacgctag ggccattaac caccgatatt
     1021 gcacctggtt atgaccatat tacctcagcg attggtgccg cgatgattgg ttggtatggc
     1081 acggc
//
LOCUS       DAOQMB010000837         1085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_128422_length_1085_cov_7.734951, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000837 DAOQMB010000000
VERSION     DAOQMB010000837.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128422_length_1085_cov_7.734951"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PLY05_14370"
     CDS             complement(<1..544)
                     /locus_tag="PLY05_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04525.1"
                     /translation="MRPFFENNVLQQQPFKPMIIVVDTTKAGSASNTFVLPIIKAASE
                     TVKIYWGDGTNSIGVNGNNTHVYAASGVYTVKIESKIFGGVYFITAGDKEKLLSIRTF
                     GRGIIRSLYHAFSGCSNLAIINDPTLINTSELCSYAFFGCSSLTALPLIDLSRATNTS
                     YMCYQCTSLSSVPLINLSNVT"
     gene            complement(544..>1085)
                     /locus_tag="PLY05_14375"
     CDS             complement(544..>1085)
                     /locus_tag="PLY05_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04526.1"
                     /translation="FLDENGELITVSSSNPLPVGGKSVRKSVSFTRPADTTAYAANDA
                     IADSTSAPTLLSFANCANANGGQGYIVKTGLLTNQKTCTARFRLSFYHTAPSPVNDNA
                     PKPMLYANKDKLIGRIDLDPCNTEDTSSDAAFTLSTALMPYVCAAADTTIYCILTTLD
                     AFTPASAQQFYLEITCELS"
BASE COUNT          329 a          215 c          222 g          319 t
ORIGIN      
        1 ttgtaacatt cgacaaatta atcagaggta cacttgagag tgaggtacac tgatagcaca
       61 tataacttgt attcgtagca cgagataaat caataagcgg caatgcagtt agtgatgagc
      121 aaccaaaaaa cgcataactg cacaactcgc ttgtatttat aagcgtagga tcgttaatta
      181 ttgctagatt agagcaacct gaaaacgcgt gataaagcga acgaataata ccgcgaccga
      241 atgttcttat agataacagt ttttctttgt ctcctgcggt aataaaataa acccctccaa
      301 aaatcttgga ctcaatcttg actgtataaa ccccactcgc tgcgtaaaca tgggtattat
      361 tcccgttaac gccaattgag tttgtgccgt caccccaata aattttaaca gtctcactag
      421 ccgctttaat aattggcagc acaaatgtat tgcttgcact gccagccttt gttgtatcaa
      481 caacaattat catcggctta aatggttgtt gctgtaagac attattctca aaaaatggtc
      541 tcattagctt aactcacagg taatttccaa gtaaaattgt tgtgctgagg caggtgtaaa
      601 cgcatcaagt gttgttaaaa tacagtagat tgttgtatcg gctgctgcac aaacgtaggg
      661 cattaaagca gttgacagcg taaaagcagc gtcagagctt gtatcttctg tgttgcatgg
      721 gtctaaatca atacgcccaa tgagtttgtc tttgttagcg taaagcatgg gcttaggtgc
      781 gttatcatta acagggcttg gtgctgtgtg ataaaacgat aatctaaaac gtgctgtaca
      841 ggttttttga ttcgtcaata aacctgtttt gacaatgtag ccctgcccac cgttagcatt
      901 tgcacagtta gcaaaactaa gcaaggttgg tgctgatgtg ctatctgcaa ttgcatcatt
      961 agccgcataa gcagttgtgt cagcaggtcg tgtgaatgat actgattttc gaacagattt
     1021 accacccaca ggcaacggat tgcttgagct aactgtaatc aattccccgt tttcatctaa
     1081 aaaag
//
LOCUS       DAOQMB010000838         1083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_128936_length_1083_cov_3.230545, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000838 DAOQMB010000000
VERSION     DAOQMB010000838.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128936_length_1083_cov_3.230545"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1083)
                     /locus_tag="PLY05_14380"
     CDS             complement(<1..>1083)
                     /locus_tag="PLY05_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005145636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNC04527.1"
                     /translation="QPQWPNFTWSDAKILPKLRQLHQKIGFLLGQSSYDSQNVELSLE
                     TLLSNIIASSAIENEKINVYSLRSSLARRLGLSAQQSHPSTQRSEGLANILFDALNNI
                     HQELTVERLFQWHEWLFAEPDWTTAKIRVGQWRGEEPMQVVSGRIDKPTIHFEAPPRS
                     DLAQQMMVFITWFNQSRHNQLLDPLLRAAISHFWFVTLHPFDDGNGRITRTLTDLALA
                     QMDRQSIRLYAMSSAILQQRQDYYRILEQSQKGNLDISEWLLWFLETLELSVNQSLQK
                     IERTLLKARFWQYFAQTDLHEAQRKVLNRLLDGGGNGFEQGISADQYQKVAKVSKATA
                     TRHLTDMLEKSCLKSIAGGGRSVRYQ"
BASE COUNT          317 a          220 c          199 g          347 t
ORIGIN      
        1 cctgataacg tacacttctc cctccacctg caatcgattt taagcaactc ttctcaagca
       61 tatcagttaa atggcgtgtg gctgttgctt tactaacttt agcaactttt tgatattgat
      121 cggcactaat gccctgctca aaaccatttc caccaccatc taacaagcga ttaagcactt
      181 tacgttgagc ctcatgcaaa tcagtttggg caaaatattg ccaaaaccgt gcttttaata
      241 aggttctttc gattttttgt aatgactgat tgacactcaa ttctaaagtc tctaaaaacc
      301 atagtagcca ttcactaata tctaaattac ctttttgaga ttgctctaat atccgataat
      361 agtcttgtcg ttgctgcaaa atagcagatg acatggcata taaacgaata ctttgcctat
      421 ccatttgtgc taaagctaaa tctgttaggg tgcgtgtgat acggccatta ccatcatcaa
      481 aaggatgtaa agtcacaaac caaaaatgac tgattgcggc tcttaataaa ggatcaagta
      541 actgattatg gcgactttga ttaaaccaag tgataaaaac catcatttgt tgtgctaaat
      601 cacttctagg aggggcttca aaatggatgg ttggtttatc tatacgtcct gataccactt
      661 gcatcggttc ttcgccgcgc cattgcccta ctcttatttt tgctgttgtc cagtcaggct
      721 ctgcaaataa ccattcatgc cattgaaata aacgctctac ggttaactct tgatgaatat
      781 tatttaacgc atcaaataaa atattagcta aaccttcgga gcgttgggta ctgggatggg
      841 attgttgtgc agatagtcct aaacgccgag ctaaggatga gcgtaaggaa tacacgttaa
      901 ttttttcgtt ttcaatggca gaggatgcaa taatattact taatagggtt tctaaactca
      961 gttcaacatt ttgactatca taactacttt gaccgagtaa aaaccctatc ttttgatgta
     1021 gttggcggag cttaggcaaa atttttgcgt ctgaccatgt aaagtttggc cattgcggct
     1081 gtt
//
LOCUS       DAOQMB010000839         1080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_129622_length_1080_cov_9.780488, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000839 DAOQMB010000000
VERSION     DAOQMB010000839.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129622_length_1080_cov_9.780488"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="murG"
                     /locus_tag="PLY05_14385"
     CDS             <1..929
                     /gene="murG"
                     /locus_tag="PLY05_14385"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNC04528.1"
                     /translation="KNGFDISYLEVQGVRGQGLARLLLAPFKILLAVIAAMKVLRAVK
                     ADCVIGLGGYVTGPGGVAARLLGKPLIIHEQNAVAGFTNKQLANIATQVLQAFPAAFP
                     ASPKVTTTGNPVRPVIANLALPEQRYGQRSGALQVLILGGSQGAVALNELVPDALAIL
                     SKEHALTIRHQTGKKQVEVTQQRYKNLGISDANVQPFINDMAEAYGWADLVICRSGAL
                     TVSELAAAGVASVLVPYPHAVDDHQTANGCYLSDEGAAILCQQVSLTAENLAQTLKPL
                     LSRPKLLQMANIARKLAQPKATATVAQFCLRS"
     gene            939..>1080
                     /locus_tag="PLY05_14390"
     CDS             939..>1080
                     /locus_tag="PLY05_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HNC04529.1"
                     /translation="MINQSSRGRLIEIPEMRRIRCIHFIGIGGAGMCGIAEVLLNQGY
                     QIS"
BASE COUNT          299 a          209 c          256 g          316 t
ORIGIN      
        1 caaaaaatgg ttttgatatt agctatttag aagtacaagg tgtgcgtggt caaggcttgg
       61 cgcggttatt acttgcacct tttaaaattt tattagcggt tattgccgcc atgaaagtgt
      121 taagagcagt aaaagcagat tgtgtgattg gtttaggtgg ttatgtcaca gggccgggtg
      181 gtgttgcagc gcggttattg ggtaaaccct taattattca cgaacaaaat gcggtagcag
      241 gttttaccaa caaacaactg gccaatattg ccacccaagt gttgcaagcc tttccagcgg
      301 cttttcctgc aagtcccaaa gtgacgacaa caggtaatcc tgtccgtcct gtaattgcga
      361 atttggcatt accagaacaa cgttatggtc aacgcagtgg cgcattacaa gtgttaattt
      421 tgggtggtag tcaaggtgca gtcgcactta atgagttagt accagatgcc ttagcgattt
      481 taagtaaaga acacgcttta actattcgtc atcaaacagg caaaaaacaa gtcgaagtta
      541 cccaacaacg ttataaaaac ttaggtatta gtgatgccaa tgttcagcca tttattaatg
      601 atatggctga agcgtatggt tgggcagatt tagtgatttg tcgttcgggg gcgttaacgg
      661 tcagtgagct tgcagcagca ggtgtcgctt cggtattagt gccatatcca catgccgttg
      721 atgaccatca aaccgctaat ggttgttatt taagtgatga aggtgcggca attttgtgtc
      781 aacaagtcag cttaacggct gaaaacttgg cacaaacctt aaaaccgtta ttatctcgac
      841 caaaattgct gcaaatggcc aatattgctc gtaaacttgc acaacccaaa gcaaccgcaa
      901 cagttgccca attttgtctt cgtagttgag tctgttttat gattaatcaa agctcgcgtg
      961 gtcgtttaat cgaaatcccc gaaatgcgcc gtattcgttg tatccatttt attgggattg
     1021 gtggcgcagg gatgtgtggt attgccgaag tattactcaa tcaaggctac caaatttcgg
//
LOCUS       DAOQMB010000840         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_130122_length_1078_cov_8.443793, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000840 DAOQMB010000000
VERSION     DAOQMB010000840.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130122_length_1078_cov_8.443793"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..>1078
                     /locus_tag="PLY05_14395"
     CDS             171..>1078
                     /locus_tag="PLY05_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04530.1"
                     /translation="METFKKTALCVLVSASLVACGGSDDDSDKVSATPASLTTTTDIA
                     KAAAGLQAAKTIADVVINNSVDTIEEVITEGKAEKDISCGTSPSAGKFKVTANTSTAY
                     TKDGTGVSSMCLESGEKFDGTLSYQCNDAKCDNASLVATNLVWGDTSKAVDLKVNGSS
                     TWSVTSMPQDDFRGSASITKSGVSTTFSSYDRLVTQYFAKNQIVGYGGLTVSGGSATN
                     CVDGTYAYSVKNSLIMNSGTQRISGGVIRISSGGNSEGPESGTVTFNSDGSVKVKLPS
                     GMEATVSKAEFESYCGLKEAYDFSEK"
BASE COUNT          352 a          166 c          252 g          308 t
ORIGIN      
        1 tttttggcat actcttactt gcaaatatca gcgtggggca tcgcctctta ttgctggtgt
       61 tagacttttg aatcaatagt ttaactaaag cgttaacagg caaaccctca ctgttgaagc
      121 taattttata cgtccattaa aattgaggga ttatctttaa aggaaaagtt atggaaactt
      181 tcaaaaaaac ggcattgtgt gtattagtat cagcctcact cgttgcttgt ggtggaagtg
      241 atgatgatag tgataaagtc tcagctaccc cagcgagtct aacgacaaca acagatattg
      301 caaaagcagc agcaggcctt caagcggcta aaacgattgc tgatgttgtc ataaataata
      361 gtgtagacac tatagaagaa gttattacag aaggtaaagc cgaaaaagac attagttgtg
      421 gtacaagtcc tagtgcaggc aaatttaaag tcactgcaaa tacaagtaca gcgtatacca
      481 aagatggtac tggcgttagt tctatgtgtt tagaaagtgg cgagaaattt gacggcacac
      541 tgtcttatca atgtaatgat gccaaatgtg ataatgcatc attagtggca actaatttag
      601 tatggggtga tactagcaag gcagttgacc ttaaagtaaa tggtagttca acatggtcag
      661 tgacgagtat gccgcaagac gattttagag gttcagcaag tattacaaaa agtggggtat
      721 caactacatt tagctcctat gacagattag tcacacaata ttttgccaaa aatcaaattg
      781 taggatacgg tggtctaact gtctctggag gaagtgctac aaactgtgtt gatggtactt
      841 atgcatacag tgttaaaaac tctctaatta tgaacagtgg tacacaacga atcagtggtg
      901 gagttattag aataagtagt ggtggtaata gcgaagggcc agaatctggt acagtgacct
      961 ttaatagtga tggtagtgtt aaagtgaaat tgccatcagg aatggaggct acagtgtcga
     1021 aggctgaatt tgagagctat tgtggcttaa aagaggctta tgatttttct gagaaata
//
LOCUS       DAOQMB010000841         1078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_130147_length_1078_cov_4.185728, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000841 DAOQMB010000000
VERSION     DAOQMB010000841.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130147_length_1078_cov_4.185728"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..913)
                     /locus_tag="PLY05_14400"
     CDS             complement(<1..913)
                     /locus_tag="PLY05_14400"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNC04531.1"
                     /translation="MNFLHKIAIVAFSVGMLTACGGGGNDTPPQLTNTSNNTFIEIKC
                     AFGEVNVSGVCRSAENICTAEAEKSWVRGYLDGFYLWYDEIPERKATDYATPRNYFDA
                     LLVKDKDRFSYTMSEEEADGFFESGLQLSYGVLWGYETDNTLRVRFVEPNSKAAQAGV
                     IRGDYVISINNQMIDTLTYQQVNELLTPKNAVPVNVKLMANYSGIIKDVTLQGENIVT
                     SPVPYYSVLPAQSNQDKVGYLLFNDHIATASDKLIEAVSYFKQQQITDLVLDLRFNSG
                     GYIYIANELAAMIGGKKTANKLFQRYVF"
BASE COUNT          317 a          235 c          169 g          357 t
ORIGIN      
        1 tgaatacata acgctgaaac aacttattgg ccgttttttt accaccaatc attgccgcta
       61 attcattggc gatatagata taaccaccac tgttaaagcg taaatccaac accaaatctg
      121 tgatttgttg ttgtttaaaa taactcactg cctcaatgag tttatcggat gccgtcgcaa
      181 tatgatcatt aaacaataaa taacccactt tatcttggtt cgattgcgca ggtaatacgg
      241 aataatatgg cacaggtgac gtaacgatat tttctccttg taaagtgacg tcctttatta
      301 tgccactata attagccatg agtttaacat tcacagggac tgcatttttt ggggttaata
      361 gctcgttaac ttgttgatat gttaaggtat caatcatttg attattaatg ctgatgacat
      421 aatcgccacg tatgacccct gcttgagcag ccttagaatt tggctcaaca aatctaacac
      481 gtaaggtatt atcggtctca tacccccaaa gcacaccata acttaactgt agcccagatt
      541 caaaaaagcc atcagcttct tcttctgaca tggtataact aaatctatct ttgtctttaa
      601 ctaacaatgc atcaaaataa ttacgcggtg tggcatagtc tgttgctttc cgctcaggaa
      661 tttcgtcgta ccacaaataa aagccatcta aataaccacg aacccatgat ttttctgctt
      721 cagcagtaca gatattttca gcagaacggc agacaccact aacattgact tcaccaaagg
      781 cacattttat ctcgatgaat gtattattgg atgtattagt taattgtggt ggagtatcgt
      841 taccgccacc accacacgct gttaacatcc caacagaaaa agcaacaatg gctattttgt
      901 gcaaaaaatt caattttaag tccttaatcc taaacattag caagccattc taacatcctt
      961 gtcatctgca atctattttt tagtggctat atcccttgct cttgagcttg tgtttttttg
     1021 ctcttcttcg cttttttctt ttttggctta tccccttttt caatatcatg ccaatgtt
//
LOCUS       DAOQMB010000842         1077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_130461_length_1077_cov_2.909980, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000842 DAOQMB010000000
VERSION     DAOQMB010000842.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130461_length_1077_cov_2.909980"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            705..>1077
                     /locus_tag="PLY05_14405"
     CDS             705..>1077
                     /locus_tag="PLY05_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04532.1"
                     /translation="MPYPARALSYQQALGTGTPQPALEANKKLLTRQDALLALLEQGR
                     MQQLAGDYTGSTQSFTQALARLDAIEQRAAISLTHTASQGTALVSNDNAIPYQSHDYE
                     RIFVHHYQALNYIALNKLEDAQ"
BASE COUNT          348 a          259 c          158 g          312 t
ORIGIN      
        1 caaactgacc attttttatt tgccaactat cagccaagca agctcttaat tcttcgtcag
       61 ataagttttg taattcttcg agttgataaa atttttcttc cattatttca ccatatgaga
      121 ctatagtcca aatttgatac atgaagaatt tgccataatt attgctttgt tcattatgta
      181 tctaactcat caatctttta gctaatgtaa acgcctgact aaaaaagttg atgattgact
      241 tatcatcctt tcaaaaacca agcaaaacta tagtcacgat tactagcacc gatgttacaa
      301 cactatcaag agatgatgca aaaatatcaa ctaataccaa tctgcaaaaa taatctaaca
      361 caacgtccac tttcttgtac cgatgctttt cacaagttcg ggctgctggg aaaacttatt
      421 ccaagcctca caacaagctg ctacagtctc atcataattc ttaaagcagc gattagacaa
      481 ataattttgt ttaagccact gccaaaagct caatgttaat actactaaaa accacatccc
      541 cacaaaccca ccacaaaaac ttatcgccta ccatttgatg cgcttttgct tatacgttaa
      601 gctcaggcaa ttactttaat catctgccaa ttatgctttt acgctaccta acgttattga
      661 cattattagg actactcaca ggctgtagca ccagcagcgt gtttatgccc tatcctgccc
      721 gtgctttaag ttatcaacaa gcacttggca caggcacgcc acaacctgcg ttagaagcca
      781 ataaaaaact cttaacccgt caagacgcac tcttagccct attagaacaa ggacgaatgc
      841 agcaacttgc aggcgactac acaggcagta cgcaaagttt tacacaggcg ttagcgcgtt
      901 tagatgccat tgagcaacgc gctgcaatat cgctcactca tactgccagt caaggcactg
      961 ccttggtatc caatgacaat gccattccct atcaaagcca tgattatgaa cgtatttttg
     1021 tccatcatta tcaagcatta aattatattg ctctcaataa attagaagat gcccaag
//
LOCUS       DAOQMB010000843         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_130859_length_1075_cov_17.506863, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000843 DAOQMB010000000
VERSION     DAOQMB010000843.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130859_length_1075_cov_17.506863"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PLY05_14410"
     CDS             complement(<1..815)
                     /locus_tag="PLY05_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04533.1"
                     /translation="MSFHQKLALVTGAGSGIGRATALLLAQLGAKVIITDRHPQSLQE
                     TQQQIQAQGGQAFCETVDVSDWVAMQALADCVHQQYGVLDILVNNAGVATSGEFLKTP
                     IEDWHWVMSINLMGVVYGCKAFGSAMAERKRGHIVNIASAAGYYAAPDMSAYSASKHA
                     VMGLSESLRIELAEFNVGVSAICPGVINTNIVATMRTNGEIVQAQAKIVDIYKKRNYG
                     PEGVAEAIVSAIKHNRAVVPVSPEAWALYAAKRLAPSVMDTVQRTSLFRKLKP"
BASE COUNT          304 a          239 c          205 g          327 t
ORIGIN      
        1 taaggcttta actttctaaa taatgaggta cgttgcactg tatccatgac tgaaggcgct
       61 aaacgtttag cggcatacaa cgcccacgct tcggggctaa caggcacaac cgcacgatta
      121 tgtttaatgg cactgacaat cgcctcagcc acaccttctg ggccataatt acgcttttta
      181 taaatatcga caattttggc ttgtgcttgt actatttcgc cattagtgcg catggttgcg
      241 acaatatttg tgttaatcac accagggcaa atggcgctca cccccacatt aaattcggct
      301 aattcaatgc gtaatgactc acttaaaccc atcaccgcat gtttactggc cgaataagcc
      361 gacatatcag gtgcagcata atagcctgcg gcagaggcaa tattgacaat atggcctcgt
      421 ttacgctctg ccatcgcact gccaaaggct ttgcaaccat acactacacc catgagatta
      481 atgctcatta cccaatgcca atcttcgatg ggtgttttta aaaattcacc cgatgtggct
      541 acgcctgcat tattcaccaa aatatctaac acaccatatt gttgatgaac acaatcagct
      601 aaggcttgca tagccaccca atcggaaaca tcaacggttt cacaaaaggc ttgaccacct
      661 tgcgcttgaa tttgttgttg cgtttcttgt aaagattgtg gatgacggtc ggtaataatc
      721 acttttgcgc ctaattgtgc caataacaac gcggtcgccc gcccaatgcc cgaacctgcg
      781 cctgtcacta aggctaattt ttgatgaaat gacataataa ccctctgtgt ataagtttaa
      841 atttggcaaa aatctagcac tatagtacca attacacaat gactgaattg acaataacta
      901 gagtatgatt ggtcaaattt ttgaggataa tatctatgat tcctgccaaa tacactaatt
      961 atgttttttc gttttttatg gcgttattaa tgtcttgtat tatgtctttg gtgattactg
     1021 tttttaatgt gggtttagta gataacttat tgtttatttg gctaaaagca tgggg
//
LOCUS       DAOQMB010000844         1075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_130860_length_1075_cov_17.003922, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000844 DAOQMB010000000
VERSION     DAOQMB010000844.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130860_length_1075_cov_17.003922"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..231
                     /locus_tag="PLY05_14415"
     CDS             31..231
                     /locus_tag="PLY05_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04534.1"
                     /translation="MNVKPTQVRGDVMEISLTKHGYQGKVKGCINGIPFCFYANGETF
                     KNVHQQLVFEVAKLRAYARLAA"
     gene            243..>1075
                     /locus_tag="PLY05_14420"
     CDS             243..>1075
                     /locus_tag="PLY05_14420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HNC04535.1"
                     /translation="MTVQARIDWLKQRQSGIGGSDIAAICGLNQYKTPMQIWESKVNP
                     ITEDNSTNEPMYWGNVLEEVVAKEYAKRTGYKVQRVNTQMRHPDFNFAIANIDRAVIN
                     PDISGNVRWKDGKLTTDRILECKTANGFAAKQWGEAGSDQVPDSYLIQCQWYLGITGA
                     SVCDLAVLIGGQDFRIYTINRDDDLINDLLQQGAAFWELVKNNIAPDPINYPEAVKKW
                     ANSDPTLSVQADDSLIDDLEQIQSIKAQVKELEAQEDEIKARVVYALKEAETLIYQGV
                     KV"
BASE COUNT          332 a          233 c          243 g          267 t
ORIGIN      
        1 ctgaatcatt gcccgactgg tctttagaat ttgaatgtca aaccgaccca agtacgtggt
       61 gatgtcatgg aaatatcatt aacaaagcat ggctatcaag gcaaagtcaa ggggtgcatt
      121 aatggcatcc ctttttgttt ttatgcaaat ggagagacgt ttaaaaacgt tcatcaacaa
      181 ctcgtttttg aagtcgctaa gcttcgtgct tatgcgcgtt tagctgctta ggagttaaaa
      241 aaatgaccgt acaagctcga attgactggc taaaacaacg ccagtctggt atcggcggca
      301 gcgatattgc tgcaatttgt ggccttaatc aatacaagac ccccatgcaa atatgggagt
      361 ctaaagttaa tccaattacc gaagacaaca gcaccaatga gcctatgtac tggggtaatg
      421 ttttagagga agttgttgcc aaagaatacg ccaagcgcac aggctacaaa gtgcagcgtg
      481 ttaataccca aatgcgacac cctgatttta actttgccat tgctaacatt gaccgcgctg
      541 ttatcaatcc cgacattagc ggtaacgtgc gctggaagga tggcaaactc actactgacc
      601 gcattttaga atgtaaaacg gctaacggct ttgcagccaa acaatggggc gaagctggta
      661 gtgaccaagt acccgactct tacctcattc agtgccaatg gtatctaggc ataacaggcg
      721 caagcgtgtg tgacctagcc gtgctgattg gcggacaaga ctttcgtatt tacacaatca
      781 atcgtgatga tgaccttatt aacgacctac ttcaacaagg tgctgctttt tgggagttgg
      841 ttaaaaacaa cattgcaccc gaccccatta actaccccga agctgtcaaa aaatgggcaa
      901 atagcgaccc tacattaagc gtacaagctg atgactcatt gattgatgac ttggagcaaa
      961 ttcaaagcat caaagcgcaa gttaaagagc tagaagcgca agaagacgag attaaggcgc
     1021 gtgtagtgta tgccctaaaa gaagctgaaa cgcttatcta tcaaggcgtt aaagt
//
LOCUS       DAOQMB010000845         1073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_131358_length_1073_cov_11.528487, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000845 DAOQMB010000000
VERSION     DAOQMB010000845.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131358_length_1073_cov_11.528487"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="PLY05_14425"
     CDS             <1..684
                     /locus_tag="PLY05_14425"
                     /inference="COORDINATES: protein motif:HMM:NF014089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HNC04536.1"
                     /translation="MMYMDNYLDVKTILGDGNGFFSALKRSLTGESLFSSLYTNNSGQ
                     NQRVAFAAPTVGKIIAINLAEVGGELICQRGAFLAGEVGTEVKLARQKKIRVGFLGGE
                     GFIIQRITGKGWVFINATGGLTEMHLAPNQTLQVDSGCWVASTSAVTYDIHYAGKVKT
                     ALFGGEGLFYASLTGPGTVWMQSLPMKRLSAELMSSAMTGRNRGGVWGRLYLIFIIIV
                     IIVSLFGGR"
     gene            686..>1073
                     /locus_tag="PLY05_14430"
     CDS             686..>1073
                     /locus_tag="PLY05_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04537.1"
                     /translation="MTERIEALVYGVGIPPTGIKVAVYFVGQRLVVDGMPIDIHISQI
                     TISVGGFEHDELFVNWQDAQQQQWAIKPIGQTQIQRLVQTAPESLQPLLKKWHQRKRH
                     LNVVWGSIAAIVLSLLLLIGVIVWQYD"
BASE COUNT          302 a          175 c          265 g          331 t
ORIGIN      
        1 atgatgtata tggataatta tttagacgtc aaaaccattt taggtgatgg taatggtttt
       61 tttagtgcgc taaaacgctc attaacaggc gagtctttat ttagtagttt atataccaat
      121 aatagcggac aaaatcagcg cgttgccttt gctgcgccaa ctgttggcaa aatcattgct
      181 atcaacttag cggaagttgg tggtgaatta atttgccaac gtggtgcttt tttagcaggt
      241 gaagtgggga cagaagttaa attagcacga caaaagaaaa ttcgcgttgg ttttttgggc
      301 ggtgaaggtt ttattattca acgtattact ggtaaaggtt gggtatttat taatgctact
      361 ggtggattaa ccgaaatgca cttagccccc aaccaaacat tacaggtaga tagtggttgt
      421 tgggtggcat caacatcagc agtgacttac gatattcatt atgcaggcaa agtaaaaacg
      481 gcattgtttg gtggtgaagg tttgttttat gccagcctga cagggccggg aacagtatgg
      541 atgcaatctt tacccatgaa acgtttaagt gccgaactga tgagcagtgc catgactggc
      601 cgcaatcgag gtggtgtgtg gggacgactg tatttaatct ttattataat cgtcattatt
      661 gtctcattgt ttggtggtcg ttaacatgac ggaacgtatt gaggcgttag tgtatggggt
      721 aggaattccg cctacaggta ttaaagttgc agtgtatttt gtggggcaac gtttagtggt
      781 tgacggtatg cccatcgaca ttcatattag tcaaataacc attagtgttg gtggttttga
      841 acatgatgaa ttgtttgtca attggcaaga tgcccagcaa caacaatggg cgattaaacc
      901 tattggtcaa actcaaattc aacgcctcgt acaaacagcc cccgaaagtt tacaaccctt
      961 attaaaaaag tggcatcaac gcaaacgaca cttaaatgta gtatgggggt caattgctgc
     1021 cattgtatta agtttgctat tgctgattgg ggtgatagtt tggcaatacg ata
//
LOCUS       DAOQMB010000846         1072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_131590_length_1072_cov_15.363815, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000846 DAOQMB010000000
VERSION     DAOQMB010000846.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131590_length_1072_cov_15.363815"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..>1072
                     /locus_tag="PLY05_14435"
     CDS             114..>1072
                     /locus_tag="PLY05_14435"
                     /inference="COORDINATES: protein motif:HMM:NF017300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HNC04538.1"
                     /translation="MIFALRDAFAELQDELARFNQQWLAHVVKANVYDVPLNNNGLAP
                     KLIPVTHYEGEEACQKAALAFQTLHFQPNQHPATAYRLAGWIGIDCDLQKEIDLVNQK
                     KDELKRLMLAIPERERNKTTRQALPNVSVLQCYRHLVQWSPAPECLYFSWAGATPSSV
                     HVSRADVEAQLERSRKNRPDQFTPSEWENIINLQKQQLAEVPPEIPLIYRWRKAPHPR
                     IMAYNKGQKSNAGRIVPANLPVFVVINSQQETPKVYGLEDFDMSQHIRETPRADSALL
                     SPVIESIGLYAQIKANKKVLINEQELHEGATTRNNEWQRKPLD"
BASE COUNT          359 a          197 c          216 g          300 t
ORIGIN      
        1 acttatctaa atacagatca agtcttgtgt tatgttgata aaacatttaa attcaataag
       61 ataaatcata tttataagcc tacatttggc taaatatagg atgaaaaata acaatgattt
      121 ttgctctacg tgatgctttt gcagagttac aagatgaatt ggctcgtttt aatcaacaat
      181 ggctagctca tgtggttaaa gcaaatgtct atgatgttcc tttaaataac aatggtttag
      241 caccaaagct aattcctgtc acacattatg aaggtgagga ggcgtgtcag aaagcagctc
      301 tcgcctttca aaccctacat tttcaaccta atcaacatcc tgcaacggct tatcgtttag
      361 ctggatggat tggtattgat tgcgatttgc aaaaagaaat tgatctggta aatcaaaaga
      421 aagatgagct aaaaagatta atgttggcca taccagagcg cgaacgtaat aaaaccacac
      481 gccaagcctt gccaaatgta tccgttttac aatgttatcg gcatttggtg cagtggtctc
      541 ctgcccctga atgtttatat ttttcgtggg caggcgcaac accaagcagt gtgcatgtta
      601 gtcgtgcgga tgttgaggca cagttagaac gttcaagaaa aaatcgtccc gatcaattta
      661 cgccgagtga atgggaaaat attattaatt tgcaaaagca acaattagca gaagtcccac
      721 ctgaaattcc gcttatttat cgttggcgaa aagcgccaca tccacgaatt atggcttata
      781 acaaaggcca aaaaagcaat gcagggcgca ttgttcctgc caacctaccc gtttttgtgg
      841 tgataaattc gcagcaagaa acgcctaaag tgtatggttt agaagatttt gatatgagtc
      901 aacatattcg agaaacacca cgagcagact cggctttatt atcacctgtg attgaaagta
      961 ttggcttata tgcgcaaatt aaagcaaata agaaagtatt gattaacgag caagaattac
     1021 acgagggtgc aacaacacgt aataatgaat ggcaacgtaa acctttagat ga
//
LOCUS       DAOQMB010000847         1071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_131851_length_1071_cov_16.469488, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000847 DAOQMB010000000
VERSION     DAOQMB010000847.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131851_length_1071_cov_16.469488"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1071)
                     /locus_tag="PLY05_14440"
     CDS             complement(<1..>1071)
                     /locus_tag="PLY05_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HNC04539.1"
                     /translation="ENSQGFSWADIPKLSQLRENLIKFQHHTWEAGVTQNTDEQIVST
                     VHSPVDKHRLLGTIYYHHLPNIEILFTQAQQRFDAWQQTPVHQRAGYLRALTVLIEEA
                     QAEILYLLTMEAGKTLPDALGEWREAIDLCRFYAQEAEKLQTSHELPHITGEHNHLSW
                     AARGVFVCISPWNFPLAIFLGQIAAAIVTGNTVIAKPANQTSLIASKIAQLLQQTGLP
                     EGVVNIIFARSSELGDYLFKHPHLAGVVFTGSTETAQHIQLALAQRRGAIIPFIAETG
                     GLNAMIADSSALAEQLVYDVMQSAFNSAGQRCSALRVLWLQEEIADTVLTRLAGQLAL
                     WQVGHSQHINSDMSAVIDGHSQQ"
BASE COUNT          316 a          213 c          229 g          313 t
ORIGIN      
        1 ttgttggcta tggccatcaa tcaccgcact catatcactg ttaatatgtt gactatgacc
       61 cacttgccaa agtgctaatt gacctgccaa acgtgtgagt actgtatcgg caatttcttc
      121 ttgtaaccat aatacacgca aggcagagca gcgttggcca gcactattaa atgctgattg
      181 catcacgtca tagactaatt gttcggcaag ggcagagctg tcagcaatca ttgcatttaa
      241 gccgccagtt tcagctataa agggaataat tgcgcctcga cgttgtgcta aagccagttg
      301 tatgtgttga gccgtttctg ttgagccagt aaaaacgacg cctgctaaat gaggatgttt
      361 aaataaatag tcacccaatt cactactacg cgcaaaaata atattgacaa caccttctgg
      421 taaacctgtt tgttgcaaca gttgagcaat tttgctagca attaatgaag tttggttggc
      481 aggtttggca atgactgtat tgcctgtaac aatggcggca gcaatttgcc ctaaaaaaat
      541 ggctaaagga aaattccacg gactaataca gacaaagaca ccccgcgctg cccaagataa
      601 atgattatgt tcgccagtaa tgtgggggag ttcatgagaa gtttgtagct tttctgcctc
      661 ttgagcataa aaacgacata aatcaatggc ttcgcgccac tcacctaaag catcaggcaa
      721 ggttttgcct gcttccatgg ttaataaata taagatttcg gcttgagcct cttcgattaa
      781 tacggttaaa gcacgcaaat aacctgcgcg ttgatggaca ggtgtttgtt gccaagcatc
      841 aaagcgttgt tgtgcttgag taaataaaat ttctatatta gggagatggt gataataaat
      901 agtgcctaat aaacgatgtt tatcaacagg actatgaacg gtgctaacaa tttgttcgtc
      961 agtattttgg gtaactcctg cttcccatgt gtgatgttga aatttaatta agttttcacg
     1021 taattgtgaa agtttgggaa tatctgccca actaaagccc tgactatttt c
//
LOCUS       DAOQMB010000848         1070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_132126_length_1070_cov_19.670936, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000848 DAOQMB010000000
VERSION     DAOQMB010000848.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132126_length_1070_cov_19.670936"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..>1070)
                     /locus_tag="PLY05_14445"
     CDS             complement(76..>1070)
                     /locus_tag="PLY05_14445"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNC04540.1"
                     /translation="PIILTGMSWISTPELVAAVSNAGGLGILALGPLTPEQTKAAIEK
                     VRQLTNKPFGVNCALLMPSAKENAQIALDLKVPVINFSLGKGDWLIKACHAYGGKVIA
                     TVTTAKHAIAAQEQGADAVMVTGHEAAAHGGEVTSLVLVPAIVDVVKIPVIACGGFAD
                     QRGVLAALALGAEAVAMGSRFATVKESPLHEHIKQQVIAKDQHETIYSPHFDGLPARY
                     MKTPLAEKLTKKPMNFVLAMVQAFFAAIQLKVPIWKVLAGILVEPQKIRLLANFGAAT
                     PKLKAATEQGDLAKGMQFIGQSQGLIHDVCSAEEMMQRLVKDLDKRLLAIQEKI"
BASE COUNT          302 a          268 c          193 g          307 t
ORIGIN      
        1 gtgagcaacc acaatcaaaa cgcaaaacaa tcctattatc tttatgcttt tttataatca
       61 aagcgataac cgtccctata ttttttcttg aatggctaat aatcgtttat ctaaatcttt
      121 gactaaacgc tgcatcattt cttcagcact acacacatcg tgaatcaagc cttgagattg
      181 accaataaac tgcataccct tggctaagtc accctgttcg gttgctgcct ttaatttagg
      241 agttgccgcg ccaaaattag ctaataatcg tattttttgt ggttctacga gaatgcctgc
      301 taacaccttc caaatgggga cttttaattg tattgctgca aaaaaagcct gaaccattgc
      361 caacacaaaa ttcataggtt ttttagtcaa cttttctgcc aatggcgttt tcatataacg
      421 tgcaggcaaa ccatcaaaat gcggtgaata aatggtctcg tgttgatctt tagcaatcac
      481 ttgttgctta atatgctcat gtaacgggct ttctttcacc gttgcaaaac gtgaacccat
      541 cgccaccgct tctgccccca acgccaaggc tgccaacaca ccacgttgat cagcaaaacc
      601 accacaagca atcactggaa tcttgaccac atcaacaatg gctggcacta agactaaaga
      661 cgttacctct ccgccatgtg ctgctgcttc atgacctgtc accatcacag catcagcacc
      721 ttgttcttga gcggctatgg cgtgtttagc agtcgtcact gtggcaatta cctttccacc
      781 ataagcgtgg caagctttaa tgagccaatc gcccttacct aatgaaaaat taatcacagg
      841 cacttttaag tctaaggcaa tttgggcatt ttctttggca gatggcatta acaaggcaca
      901 attcacccca aagggtttat ttgttaattg gcgcaccttt tctatggcag ccttagtttg
      961 ttcgggtgtc aacggcccta aagctaaaat acctaagcct ccagcattgc tcaccgcagc
     1021 aaccagttca ggggtagaaa tccaactcat gcccgttaaa ataatggggt
//
LOCUS       DAOQMB010000849         1068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_132596_length_1068_cov_18.281343, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000849 DAOQMB010000000
VERSION     DAOQMB010000849.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132596_length_1068_cov_18.281343"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..985
                     /locus_tag="PLY05_14450"
     CDS             <1..985
                     /locus_tag="PLY05_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNC04541.1"
                     /translation="DDDKDGIKNSVDKCPNTPANSKVDDKGCPIDGDDDKDGIKNSKD
                     KCPNTPINTKVDDKGCPIDGDDDKDGIKNSKDKCPNTPINTKVDDKGCSLSANTGGNS
                     NNWTGKPSGGNSTSYSGGGAAAPINVTVTNNNYVNGINQSDKDQDGINDDEDKCPSTP
                     LNTKVDVRGCPVNVVIDDDLDKDGVKNAQDSCPNTPLGTKVDAKGCPLVLLEDVEIKL
                     NVLFATDKATIVDNAFVDILKVADFMRQYPNVTVVIEGHTDDRASIAHNQKLSQRRAE
                     AVRRELIRFGVDARRLLAIGYGELRPIADNTTEQGRLQNRRVVAVAKAQKQ"
BASE COUNT          376 a          164 c          243 g          285 t
ORIGIN      
        1 tgatgacgat aaagacggca ttaaaaactc tgtagataaa tgccctaata cacctgctaa
       61 tagcaaagtt gacgacaaag gttgtccgat tgatggtgat gatgacaaag atggcatcaa
      121 aaactcaaaa gataaatgcc ccaatacccc tataaatacc aaagttgatg ataaaggctg
      181 tccgattgat ggtgatgatg acaaagatgg catcaaaaac tcaaaagata aatgccccaa
      241 tacccctata aataccaaag ttgatgataa aggctgttct ttaagtgcta atacaggcgg
      301 aaatagtaat aattggacag gtaagcctag tggcggaaat agtacgagtt atagtggggg
      361 tggggcggct gcaccaatta atgtcactgt tactaacaat aactatgtga acggtattaa
      421 tcaaagtgat aaagatcaag atgggattaa tgatgatgaa gataaatgtc caagtacccc
      481 attaaacact aaagttgatg ttaggggctg tcctgtcaat gttgtcattg atgatgactt
      541 agataaagat ggtgtcaaaa atgcacaaga tagttgtcct aatactcctt taggaactaa
      601 ggttgatgct aaaggatgtc cattagtatt gttagaagat gtagaaataa aacttaatgt
      661 gttatttgcc acagataaag ccactattgt tgataacgcc tttgttgata ttttaaaagt
      721 tgcagatttt atgcgtcagt atcccaatgt cacggttgtt atagaagggc atactgacga
      781 cagagcgagt attgcacata accaaaaact atcacaaaga cgtgcagaag ctgttcgtag
      841 agagctaatt cgttttggcg ttgatgctag acgtttgtta gcgattggct atggtgaatt
      901 gagaccaata gcggataata caacagagca aggtcgctta caaaatagaa gagtggttgc
      961 tgttgccaaa gctcaaaaac aataaaatag gagaaaaaaa tgccgtttcg taatagagta
     1021 gtgtggatta caggtgcatc gagtggtatt ggtgaagcat tagcctat
//
LOCUS       DAOQMB010000850         1067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_132829_length_1067_cov_7.678854, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000850 DAOQMB010000000
VERSION     DAOQMB010000850.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_132829_length_1067_cov_7.678854"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="PLY05_14455"
     CDS             <1..290
                     /locus_tag="PLY05_14455"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HNC04542.1"
                     /translation="EEAISRLEQAIDSLNEDLPPLKDFILPAGGKAASTCHIARTVCR
                     RAERRVFSLSQQEQISPLALQYLNRLSDYLFVAARILAREHGGSEVLWQRS"
     gene            complement(287..955)
                     /locus_tag="PLY05_14460"
     CDS             complement(287..955)
                     /locus_tag="PLY05_14460"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HNC04543.1"
                     /translation="MIIIKKLTILSVVAALAACQSIGLKPQQTLKATINNNQTSYVSS
                     IAQDGTSVVVKLANGQQRVVTSNLPTYSATIGRVNVQQGGRSFAIEPTQPTVTTTGKV
                     TGSAFKPNAPEGAHWQLIKLNGQKVDSTNSKRPPYLRMVGGQMHGLTGCNVMSGIYHR
                     DDKKMQFMRVQGTTSSCDYQELERSFIGALQRLRLWRVTPDYHLQFLDEKNQVVAEFI
                     ALFP"
     gene            complement(952..>1067)
                     /locus_tag="PLY05_14465"
     CDS             complement(952..>1067)
                     /locus_tag="PLY05_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009585510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HNC04544.1"
                     /translation="ILPLSLSYDHRVIDGADAARFTTLLSQILADIRRVLL"
BASE COUNT          303 a          237 c          204 g          323 t
ORIGIN      
        1 aagaagaagc aatctcacgt ttagagcaag cgattgactc tttaaatgag gatttacccc
       61 ccttaaaaga ttttatttta cccgcaggtg gcaaagcagc aagtacttgt catattgcgc
      121 gcactgtctg ccgtcgtgct gaacgtcgag tatttagcct tagccaacaa gaacaaattt
      181 cgcctttagc cttacaatat ctaaatcgtt tatctgatta tttgtttgtg gcagcacgga
      241 ttttagcgcg tgaacatggc ggctctgaag tattatggca acgctcttag ggaaacaatg
      301 cgataaactc cgcaaccacc tgattttttt catctaaaaa ctgcaaatga taatcaggtg
      361 ttactcgcca caaccgcaaa cgctgtaacg caccaataaa actccgttct aattcttgat
      421 aatcacaact actggttgtg ccttgcacac gcataaactg cattttttta tcatcacgat
      481 gataaatacc gctcatgaca ttacagccag ttagcccgtg catttgtcca cccaccatac
      541 gtaaataagg ggggcgttta ctgtttgtgc tatcaacttt ttggccattt agcttaatta
      601 attgccaatg cgcgccttcg ggagcatttg gtttaaaggc actgcctgtc actttgcctg
      661 ttgttgtgac agtgggctga gtaggctcaa tcgcaaaact ccgtccacct tgctgcacat
      721 taaccctacc aatagttgct gaataagtgg gcaagtttga ggtcacaact cgttgctgac
      781 cattagctaa tttaaccaca actgatgtgc catcttgagc aatactactc acataacttg
      841 tttgattgtt attaatagtc gcttttagag tttgctgcgg tttaagccct atagattggc
      901 aagcagctaa tgcggccaca acactaagta ttgttaattt ttttattatt atcataataa
      961 aactcgtcgt atatcggcca atatttgact taataaagtc gtaaatctgg ctgcatcagc
     1021 accatcaatt acccgatggt cgtaagataa agaaagtggc aatatta
//
LOCUS       DAOQMB010000851         1066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_133049_length_1066_cov_13.702275, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000851 DAOQMB010000000
VERSION     DAOQMB010000851.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133049_length_1066_cov_13.702275"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..84
                     /locus_tag="PLY05_14470"
     CDS             <1..84
                     /locus_tag="PLY05_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003656469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically inducible protein OsmC"
                     /protein_id="HNC04545.1"
                     /translation="AEKYCSASAMLSAGGVVITHDYEIVTE"
     gene            90..560
                     /locus_tag="PLY05_14475"
     CDS             90..560
                     /locus_tag="PLY05_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04546.1"
                     /translation="MFRLLQIVCLFALSIGSVWADDLQKAFNVVKHHEKQQGNIFVPY
                     AEQCQGQEGFYSPLSQVLTIDKRQKAILFTAFSCIGNGVAKRLVMIKHDKYQLIKNFK
                     MADMDFLGDMAYLENRTIVFNGYKWTDKDGHCCPSQKAALHFNVDNFKQELIIE"
     gene            complement(550..1026)
                     /locus_tag="PLY05_14480"
     CDS             complement(550..1026)
                     /locus_tag="PLY05_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HNC04547.1"
                     /translation="MFKTLFPKSLKLKLLLNTYLPFLGAGIQIVHVSDDFLEIDVKMP
                     LHKGNRNYVGTHFGGSLYAMTDPFLMLMLMENLGKDYIIWDKAAHIDFISPGRGTVYA
                     QFRLSPKEIGQVKVAAESGKAICPEYTVEVLGEDGKVVAKVIKTLYVKKKQRTITQ"
BASE COUNT          345 a          179 c          205 g          337 t
ORIGIN      
        1 gccgaaaagt attgttctgc gtcggcgatg ttaagtgcag gtggtgtggt gattacccac
       61 gattatgaga ttgtgactga gtaactatta tgtttagact tttgcaaatt gtttgtctgt
      121 ttgctttaag tattggctca gtatgggcag atgatttgca aaaagccttt aatgttgtga
      181 agcatcacga aaaacaacaa ggtaatattt ttgttccata cgcagagcaa tgccaagggc
      241 aggaaggatt ctattcaccc ttgtcacaag tgttaaccat tgataaacgc caaaaagcaa
      301 ttttatttac agcatttagt tgtataggta atggggttgc caaacgctta gtgatgatta
      361 aacacgataa atatcaactg attaaaaatt ttaaaatggc agatatggat tttttgggtg
      421 atatggctta tttagaaaat agaaccattg tgtttaatgg ctataaatgg acagataaag
      481 atggacattg ttgcccatct caaaaagcgg ccttacattt taatgttgat aattttaagc
      541 aagaactgat tattgagtaa tagtacgttg tttctttttg acatataacg tcttaatgac
      601 tttagcgact actttgccat cttcacctaa aacttcaacc gtatattcgg ggcaaatagc
      661 tttaccactt tcggccgcaa ctttaacttg gccaatttct ttggggctaa gtctaaattg
      721 agcataaaca gtgccacgac caggactaat aaaatcaata tgcgcggcct tgtcccaaat
      781 gatgtagtct ttgcccaaat tttccatgag cattaacatc aaaaaagggt cagtcattgc
      841 atataagcta ccaccaaaat gagtgccaac ataattgcga ttacctttat gtaagggcat
      901 tttgacgtca atttctaaaa aatcatcact gacatgcaca atttgaatgc ctgcacccaa
      961 aaaaggtaaa taggtattta ataataattt gagtttgagt gatttgggaa ataaggtctt
     1021 aaacataaaa actcgttttg gtcatcactt tagaaacaat gcccat
//
LOCUS       DAOQMB010000852         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_133320_length_1065_cov_12.000000, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000852 DAOQMB010000000
VERSION     DAOQMB010000852.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133320_length_1065_cov_12.000000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..542)
                     /locus_tag="PLY05_14485"
     CDS             complement(342..542)
                     /locus_tag="PLY05_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04548.1"
                     /translation="MITDTEIKLKGVEALIVALGEVQAERFISLILREPFDYTLWQQK
                     LWLNQSVESISSMAMAHRQVVK"
     gene            complement(532..>1065)
                     /locus_tag="PLY05_14490"
     CDS             complement(532..>1065)
                     /locus_tag="PLY05_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04549.1"
                     /translation="SMLNRLVLLIFIVTANSGSHLLSDLSGIVQLMKVYLDNCMFNRP
                     FDNQTNLRVRIETEAKLYVQDKIKLGDLGLVWSYILDFENFQNPFVERKQAIAKWRNF
                     ANVDIEETALLLANAQFFVSLGIQPKDALHVACAIEGQADYFLSTDDKLLKKLVTQHQ
                     IIAMNPVDFIKVLENDY"
BASE COUNT          342 a          227 c          176 g          320 t
ORIGIN      
        1 ctccaataag cttggctcat aatggtactc aagaattttg atacagtcag ataataagct
       61 aagtaacagg gatatcaaat taggcatgat ttcctgtatt ttgctatgct gcatacaggc
      121 tacaaaccta cctcccataa tttaggggag gaagcaaaaa ccagaggtag ggtgcattag
      181 cgacagcgta aggcaccttt tttattcatc attttcatta aaaatgatgc aatgcccttc
      241 ggttattgca tcctactcgt gctaatcgcc acttgcccta acgaaagctc agactgctgt
      301 ttgttaggca aaaatggcac attgaattca atctacgtga attacttaac cacttggcga
      361 tgcgccattg ccatactgct aatgctttca acagattgat ttagccataa cttttgttgc
      421 cacagggtat agtcaaatgg ctctcgcaga attaagctaa taaaacgctc tgcttgaact
      481 tcccccaaag ctacaattaa agcctcaacg ccctttagct tgatttcggt atcagtaatc
      541 attttcaagc accttaataa aatctacggg gttcattgct attatttgat gttgtgttac
      601 taacttttta agcaatttat cgtctgttga taaaaaataa tcagcttgcc cttcgattgc
      661 acaagctaca tgtaatgcgt ctttcggttg aatacccaaa gaaacaaaaa attgtgcatt
      721 tgccaataat aaagccgttt cctcaatatc aacattagca aaatttcgcc atttagcaat
      781 ggcctgttta cgctccacaa atgggttttg gaaattttca aaatccaaaa tgtaagacca
      841 aaccaagccc aaatcaccta atttaatttt atcttgaaca tataacttcg cctctgtctc
      901 aatacgtacc cgtaaattgg tttgattgtc aaaaggccga ttaaacatac aattatcaag
      961 ataaactttc ataagctgca caataccact caaatcagat aataaatggc tgccactgtt
     1021 ggctgtgact atgaaaataa gcaagactag tcggttcaac atcga
//
LOCUS       DAOQMB010000853         1065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_133321_length_1065_cov_7.354455, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000853 DAOQMB010000000
VERSION     DAOQMB010000853.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133321_length_1065_cov_7.354455"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /gene="pglZ"
                     /locus_tag="PLY05_14495"
     CDS             <1..980
                     /gene="pglZ"
                     /locus_tag="PLY05_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HNC04550.1"
                     /translation="VDGQSSKGSDNRKKILNKRLSASDLILAKDFTSLKQDDSRALVR
                     DNDVLYIYHNKIDETGVLATEEQVFDATEETITEMMSLIKKLTNANVNNILLTADHGF
                     IYQYQALAESDFLSSDPQASTVLYRNRRFLLGKGFQPHPSFKHFHSSQLGLVGDVEVM
                     LPKSINRLRLQGSSSRFVHGAASLQEIVIPLLHINKKRQSDLSQVDIEVLRGSANKIT
                     TNHLSVMLYQLQPVTDKCRPRTLRIGLYSEAGALISDSHEVIFDRQADNPREREIALR
                     LMLTSQADNLNGQTVSLMLEEQIEGTSHYKPYTALNYLMQRSFTRDFDF"
BASE COUNT          318 a          231 c          211 g          305 t
ORIGIN      
        1 ttgttgatgg ccaaagtagc aaaggcagtg ataaccgtaa aaagatactc aataaacgcc
       61 tctcagccag tgatcttatt ttggctaaag actttacgtc cttaaagcaa gatgattcaa
      121 gagccttagt gcgtgataat gatgttttgt atatctatca caataaaata gatgaaactg
      181 gtgttttggc aacagaagaa caggtttttg atgccacaga agaaactatc acagaaatga
      241 tgagtttgat taaaaaactg actaatgcca acgtcaacaa cattttactg accgcagatc
      301 atggttttat ttatcaatat caagcccttg ccgaaagtga ttttttatcc tcagacccac
      361 aagcctcaac ggtgttatat cgcaatcgac gctttttatt aggtaaaggc tttcagcccc
      421 atccctcgtt taagcacttc cacagttcgc aattgggctt agtgggtgat gtggaagtga
      481 tgttacccaa gtcgattaac cgtttacgct tacaaggctc aagcagtcgc tttgtacatg
      541 gcgcagccag cttacaagaa attgttatcc ctttactgca catcaataaa aagcggcaaa
      601 gtgatctgtc acaagttgat attgaagtgc tacgcggttc ggctaataaa attaccacta
      661 accatctctc cgttatgctc tatcaactac aaccagtcac tgacaaatgt cggcctcgca
      721 ccttgcgtat tggtttgtat agtgaagcag gcgcattgat ttctgatagc catgaggtga
      781 tttttgatcg tcaggcagac aatccccgtg aacgtgaaat tgctttacgc ctcatgttga
      841 ccagtcaagc cgacaacctc aacggccaaa ctgtttcttt aatgttagaa gagcaaattg
      901 aaggcacatc ccattacaaa ccgtacactg cactcaatta cttgatgcag cgttccttta
      961 cccgtgactt tgacttttag agcaacccct tatgagccag cttgatgata aaattaatga
     1021 gcatttttcg ggtttagtgg tgcgtaaaga cctcgtaaaa acagt
//
LOCUS       DAOQMB010000854         1064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_133586_length_1064_cov_10.664024, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000854 DAOQMB010000000
VERSION     DAOQMB010000854.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133586_length_1064_cov_10.664024"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLY05_14500"
     CDS             <1..872
                     /locus_tag="PLY05_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HNC04551.1"
                     /translation="FIWLHFNLTNTNTEKWLQRHLPLSPIFYEALHEGTRSTRIEYAD
                     DSLIAVINDVVFDFNFEASDVSTLWLSVQNNVVISARVKPLLSIDHLRAAVKHGEIFN
                     SPTDLLVHLLREQADVLTQIVRNIAVHVDHIEDRLLANRLENNRANLANARRLLLRLQ
                     RLLAPEPSALFRLLNRPPMWMIEQDVLDLRQSTEEFSTVLRDMNTLQERIKLLQEEIA
                     AYINEQNNRSLFVLTTVTVLALPINITAGLFGMNVGGIPLANDHAGFWIVVFLVAILT
                     CIAVWFAFGKNRD"
BASE COUNT          301 a          210 c          205 g          348 t
ORIGIN      
        1 aatttatttg gctgcatttt aatttaacca ataccaatac cgaaaaatgg ctacaacgcc
       61 acctaccttt atcgccaatt ttttatgaag ctttacacga aggcacacgc tcaacacgca
      121 ttgaatatgc cgatgacagt ttaattgcgg tgattaacga tgtggttttt gattttaatt
      181 ttgaagcctc tgatgtttca accttgtggc tcagtgtgca aaataatgtg gtgattagtg
      241 cgcgtgttaa acccttatta tctatagatc atttacgggc ggcggttaaa cacggtgaaa
      301 tttttaactc acccaccgat ttattagtac atttattacg tgaacaagcc gatgttttaa
      361 ctcaaatagt gagaaatatt gcggttcatg ttgaccatat tgaagataga ttattagcta
      421 atcgtttaga aaacaatcgt gctaatttag ccaatgcacg acgtttgtta ttaagattac
      481 aacgattatt agctcccgaa ccttcggcat tatttcgttt attaaatcgc ccaccaatgt
      541 ggatgattga gcaagatgtt ttagacttac gccaatccac tgaagagttc tccacggtgc
      601 tgcgtgatat gaatacctta caagaacgga ttaaactatt acaagaagaa attgccgcct
      661 atattaacga acaaaataat cgtagtttgt ttgtgttaac caccgtcacc gtattagctt
      721 taccgattaa tattaccgca ggtttatttg gcatgaatgt tggcggcatt cctttagcaa
      781 acgatcatgc tggtttttgg attgtggtat ttttagtggc gattttgact tgtattgcgg
      841 tgtggtttgc ttttggcaaa aatagagatt agttcagccc tcaccccaac cctctcccac
      901 aggagaggga gtcctctctt cagtaggaga acgctagcgt tagggctaat aacgattaac
      961 tcgctaattt ctgcatccac acttttaacg gcgttaatgc ctctatggct gcatcttctt
     1021 gccaataaat actaatacta ttggcctgag cttctatagc aatt
//
LOCUS       DAOQMB010000855         1063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_133855_length_1063_cov_6.230159, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000855 DAOQMB010000000
VERSION     DAOQMB010000855.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133855_length_1063_cov_6.230159"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..428)
                     /locus_tag="PLY05_14505"
     CDS             complement(48..428)
                     /locus_tag="PLY05_14505"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNC04552.1"
                     /translation="MSKAFLLDTHTFLWMASDDLRLKAAEKHIMAAEQLYLSMASWWE
                     IAIKINIGKSSYDYADLLTIAKKMNLDFLTITPQHGQTLLTLPLIHRDPFDRMLIAQS
                     ITESLPLLTHDETLASYTKLVTLF"
     gene            complement(421..702)
                     /locus_tag="PLY05_14510"
     CDS             complement(421..702)
                     /locus_tag="PLY05_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04553.1"
                     /translation="MITLPVTPSNSIELSALLIDQIEHGEIVTLTKNGQAIAQILPVK
                     YTTPTLTVESRMVGKWPIGLLKNKMQLDGFDEPLPDDLIDAFEGKYKNV"
     gene            complement(768..>1063)
                     /locus_tag="PLY05_14515"
     CDS             complement(768..>1063)
                     /locus_tag="PLY05_14515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004994281.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HNC04554.1"
                     /translation="RRQKWIDQAQSLNLYISHASGKKLDVTYRMAWYRGLKTTYYLRA
                     IGATAAEKSTISDGALNAVKAQVQVVHEESAFVEAAPVPMACSIDNPDCEACQ"
BASE COUNT          317 a          210 c          212 g          324 t
ORIGIN      
        1 ttttcttcgg cataaaaatc atcccaattt aacatggtgg ttctctctta aaatagcgta
       61 acaagtttgg tgtagctggc caatgtttca tcatgtgtaa gcagtggcaa agattctgtg
      121 atgctttgag caattaacat tcggtcaaat gggtcacgat gaatcaacgg caacgtgagc
      181 aaagtttgac catgttgtgg tgtaattgtc aaaaagtcca aattcatttt tttagcaatc
      241 gtgagcaggt cggcataatc ataagacgat ttaccaatgt tgattttaat ggcaatttcc
      301 caccacgaag ccatacttag atacaattgc tccgcagcca tgatgtgttt ttcggcggct
      361 ttgaggcgta aatcatcaga agccatccat aaaaacgtat gggtatctaa caaaaaagcc
      421 ttagacattt ttatatttgc cttcaaacgc atcaattaag tcatcaggca gtggttcgtc
      481 aaaaccatct aattgcattt tgtttttgag taaacctatt ggccatttac caaccatacg
      541 actttcaact gttagtgtag gcgtagtgta tttaacaggt aaaatttggg caatcgcctg
      601 accatttttt gtaagagtga cgatttcacc atgctcaatt tggtctatca ataatgccga
      661 taactcaata gagttagagg gggtaacagg tagagtaatc atgtgatcct cgcatacaac
      721 ggataagact aagatggtat taaaaacatc ttagtcttta taacggctta ctgacaagct
      781 tcacaatcag gattatcaat actacaagcc attggtacag gtgcagcttc tacaaaagca
      841 gactcttcat gtacgacttg tacttgcgct ttaactgcat ttaatgcgcc atcactaatg
      901 gtcgattttt cagcagccgt tgcaccaatt gcacggaggt aataggtggt ttttaaacca
      961 cgataccaag ccatacgata agtcacatcc aactttttgc ccgatgcgtg gctaatgtac
     1021 aagtttaagg attgtgcttg gtcaatccat ttttgacgac ggc
//
LOCUS       DAOQMB010000856         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_134095_length_1062_cov_8.325720, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000856 DAOQMB010000000
VERSION     DAOQMB010000856.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134095_length_1062_cov_8.325720"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLY05_14520"
     CDS             <1..938
                     /locus_tag="PLY05_14520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC04555.1"
                     /translation="AFSLLAKLSHILAPQSTEFNSPLAVRSQAALNGVSGDKLEFWDS
                     PLAISLSLRGEHGDFLDLDSWANDPAKGHVIFVHGLCESDIDWQRGEHHQQFYHELAQ
                     SGYKVAWLRYNTGRAIHHNGEEFAELLQNHFANKGKSVMLIGHSMGGLVIRSAMDWAE
                     KQQHTWLSRISHAAYLGTPHLGAPLERLGNKANNLLNITPYTRPFMRLGNIRSRGIKD
                     LRYARITEDNIVPHLPEHIEHLLVASTWKSEYDYDVVGDGLVPMSSALAQDDQGDVLF
                     APKVTRVTLNDMSHIAMLSDERIYQQLRQWLKLFV"
BASE COUNT          297 a          197 c          227 g          341 t
ORIGIN      
        1 ctgctttttc tttattagcc aagttaagcc atattttagc tcctcaatcc acagaattta
       61 atagtccctt agcggtgcgt tcacaagctg cgcttaatgg tgtctcgggt gacaaattag
      121 aattttggga tagcccgtta gctatttcat tatcgttacg tggtgaacat ggtgattttt
      181 tagatttaga ttcttgggct aatgatcctg ccaaaggcca tgtgattttt gttcatggtt
      241 tatgtgaaag tgacattgat tggcaacgtg gcgaacatca tcaacagttt tatcatgagt
      301 tagcacaatc aggctataaa gtagcgtggt tacgttacaa tacgggtcgg gctattcatc
      361 ataatggtga ggagtttgca gaactattgc aaaaccattt tgccaacaaa ggcaaatctg
      421 tgatgttaat tggtcatagt atgggtggtt tggtcattcg tagtgcaatg gattgggcag
      481 aaaaacaaca gcacacatgg ttaagccgta ttagccatgc ggcctattta ggaactcctc
      541 acttaggcgc accgttagaa cgtttaggca ataaagccaa taacttatta aacattaccc
      601 cttatactcg tccttttatg cgtttaggca atatccgtag tcgtggcatt aaagatttac
      661 gttatgcacg aattaccgaa gacaatattg tcccgcattt acctgagcat attgagcatt
      721 tattagttgc aagcacttgg aaatctgaat acgactatga tgttgttggt gatggtttag
      781 tgcctatgag cagtgcctta gcccaagatg atcaaggcga tgtgttgttt gcaccaaaag
      841 tcactcgtgt cacgcttaat gatatgagtc atattgctat gctcagtgat gaacgcattt
      901 atcaacaact ccgtcaatgg ttaaagttat ttgtatgaat actgccgatt tagctgcttt
      961 tatcgaagtt gctcgttttg aatcattttc tttagcagcc gaaaaactac atttaaccca
     1021 acctgcgatt agtaaacggg tacaaaattt ggaagaaacc tt
//
LOCUS       DAOQMB010000857         1062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_134097_length_1062_cov_7.538232, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000857 DAOQMB010000000
VERSION     DAOQMB010000857.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134097_length_1062_cov_7.538232"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /locus_tag="PLY05_14525"
     CDS             <1..938
                     /locus_tag="PLY05_14525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNC04556.1"
                     /translation="AFSLLAKLSHILAPQSTEFNNPLAVRSQAAFNGVSGDKLEFWDS
                     PLAIPLSLRGENGDFLDVASWATDPAKGYVIFLHGLCESDLDWQRGQHHQDFYHELAQ
                     SGYKVAWLRYNTGRAIHHNGEEFAQLLQDHFANQGKPIVLIGHSMGGLVMRSAMDWAE
                     KQQHTWLSRISHAAYLGSPHLGAPLERLGNKANNLLNITPYTRPFMRLGNIRSRGIKD
                     LRYARITEDNIVPHLPEHIEHLLIASLWKSELNYDVLGDGIVPMSSALAQDEQGDVLF
                     APKLNRVTFSDMSHLAMLSDERIYQQLRQWLKFDL"
BASE COUNT          288 a          198 c          231 g          345 t
ORIGIN      
        1 ctgctttttc tttattagcc aagttaagcc atattttagc tcctcaatcc acagagttta
       61 acaatccttt agcggtgcgt tctcaggctg cttttaatgg ggtttctggc gataaattag
      121 agttttggga tagtccttta gccattcctt tatctttgcg tggtgaaaac ggcgattttt
      181 tagacgtagc gtcttgggca actgacccag ccaaaggtta tgtgattttt ttacacggtt
      241 tatgtgaaag cgatttagat tggcaacgtg gtcaacatca tcaagatttt tatcatgagt
      301 tagcacaatc aggctacaaa gtcgcatggt tacgctataa cacagggcgt gctattcatc
      361 ataatggcga agaatttgcc caattattac aagatcattt tgccaaccaa ggcaaaccca
      421 tcgtgttaat tggccatagt atgggtggtt tggtgatgcg tagtgcgatg gattgggcag
      481 aaaaacaaca acatacttgg ttaagccgta ttagccatgc cgcttatttg ggtagtccac
      541 atttgggtgc gcctttagaa cgtttgggca ataaagccaa taacttatta aatattaccc
      601 cttatactcg cccttttatg cgtttaggca atatccgtag tcgtggcatt aaagatttac
      661 gttatgcgcg aattaccgaa gacaatattg tgccgcattt acctgagcat attgagcatt
      721 tattaattgc tagcttgtgg aaatctgaac tcaattatga tgttttaggc gatggcattg
      781 tgccaatgag cagtgcttta gcccaagatg agcaagggga tgtattgttt gcccctaaac
      841 ttaatcgtgt gacgtttagc gatatgagcc atttagccat gcttagtgat gaacgcattt
      901 atcaacagtt gcgccaatgg ttaaagtttg atttatgaat actgctgatt tagccgcttt
      961 tattgaagtt gcccgttttg aatcattttc attagcggct gaaaaattac atttaaccca
     1021 acctgcgatt agtaaacggg tacaaaattt ggaagaaacc tt
//
LOCUS       DAOQMB010000858         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_134886_length_1059_cov_9.182271, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000858 DAOQMB010000000
VERSION     DAOQMB010000858.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134886_length_1059_cov_9.182271"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1059)
                     /locus_tag="PLY05_14530"
     CDS             complement(<1..>1059)
                     /locus_tag="PLY05_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04557.1"
                     /translation="TIGFGGVFVVLAITLIFVYLLVESWPLFKQAEFEKAQSYQAQQQ
                     AIWVDMDEYNQTGIRVLATGEVQYFDVKTGQTTSTEQMTLPQGVSISTVERSGVLQGL
                     AGAGLSNGQLLVFKANYSVDVAEDGKSKTVTPSIRFPYGTTPLVVNDVPQPLTEFALQ
                     GGEDSLQVMAAANNQLLWQRFAVHKQEGEAGSFGLGSAMDEEDSQDKVFERVSNMGLA
                     VDSQAIRQIILGQEARWAYVLTETDISVFKITDGQPKLYQTLKAALGNAKITAAIPLQ
                     GEISLLVGDNKGAISQWFLVRDQASSEDKFSLEKIRSFQLGSAPITTIAPEAKRKGFV
                     AGDSQGKIGYFYTTSERT"
BASE COUNT          310 a          257 c          176 g          316 t
ORIGIN      
        1 tagtacgctc agatgtcgta taaaaataac cgatttttcc ttggctatcg ccagcaacaa
       61 aacctttacg tttggcttct ggtgcaatgg tagtaatcgg tgcagaacct aactggaaac
      121 tacggatttt ttctagacta aatttatctt cgctactagc ttgatctcta accaaaaacc
      181 actggctaat agcccctttg ttatcaccca ctaatagcga tatttctcct tgcaaaggaa
      241 tagctgcggt aattttagca ttccctaaag ccgctttaag cgtttgataa agcttaggtt
      301 gaccatcggt aattttaaat accgaaatat cagtctcggt taacacatac gcccaacgtg
      361 cttcttgtcc caaaataatt tgacgaatgg cttggctatc cactgccagc cccatattac
      421 tgacacgctc aaaaacttta tcttggctgt cttcttcatc cattgcacta cctaaaccaa
      481 aactacctgc ttcgccttct tgcttatgca cagcaaaacg ttgccataat aattggttat
      541 tggcggctgc cataacttgt aaagaatctt caccaccttg taacgcaaac tctgttaatg
      601 gttgtggtac atcatttacc actaaaggtg ttgtgccata ggggaaacga atgctggggg
      661 taacggtttt agacttacca tcctcggcaa catcgacaga ataattggct ttaaatacca
      721 ataattgccc attagataag cctgcacccg ctaatccttg taacacacca ctacgctcaa
      781 ccgtactaat gctcactcct tgaggcaaag tcatttgttc tgttgaagtg gtttgtcccg
      841 ttttaacgtc aaaatactgt acttcacctg tggctaatac acgaatacct gtttgattgt
      901 attcatccat atcaacccaa atagcctgtt gttgcgcttg ataactctgt gctttttcaa
      961 actcggcttg tttaaataaa ggccaactct ctactaataa ataaacaaaa atcaaggtaa
     1021 tggctaacac cacaaataca ccgccaaaac caatcgtca
//
LOCUS       DAOQMB010000859         1059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_134897_length_1059_cov_5.216135, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000859 DAOQMB010000000
VERSION     DAOQMB010000859.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134897_length_1059_cov_5.216135"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLY05_14535"
     CDS             <1..625
                     /locus_tag="PLY05_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HNC04558.1"
                     /translation="GLKGVGKSTLLDALCLRLQQENVRFSLVTPTRPLTTFCLAEFLA
                     KHCRLLVKFDIFRRYLYRYRSNQHARQADWNQPLILGDRSILTSLVTRWPTDCDYQQV
                     QYIQQTKAQEHEIPWPDEVIYLEMPLEPLLQRLQKRQRDYGKQDECVERLSLAFEAYQ
                     DLQHKGRFLGVPSELQWSIVKADQTPKEVVEQVYAVLKARCPKAWPC"
     gene            890..>1059
                     /locus_tag="PLY05_14540"
     CDS             890..>1059
                     /locus_tag="PLY05_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HNC04559.1"
                     /translation="MPHTFVNPFTDFGFKRLFGTEPNKGLLISFLNTLLPAKHQIQDL
                     QYTKNEYQGYTV"
BASE COUNT          317 a          200 c          217 g          325 t
ORIGIN      
        1 aggtttaaaa ggcgtgggaa aatcaacttt gttggatgcc ttgtgtttac gtttacaaca
       61 agaaaacgtt cgttttagtt tagtaacacc aacgcgtcca ttaaccactt tttgtctggc
      121 tgagtttttg gctaagcatt gtcggttgct cgtgaaattt gatatttttc gccgttattt
      181 atatcgttat cgttcaaacc aacacgcacg ccaagcagac tggaatcaac ccttgatatt
      241 gggtgatcgg agcattttaa ccagtttagt gacacgttgg ccgacagact gtgactatca
      301 gcaagtacag tatattcaac aaacaaaagc gcaagaacac gaaattcctt ggcctgatga
      361 ggtgatttat ttggaaatgc ccttggagcc tttattacaa cggctacaaa aaagacagcg
      421 agattatggc aaacaggatg agtgtgttga acgtttaagc ttggcgtttg aggcttacca
      481 agatttacag cataaaggca gatttttagg tgttccctcg gaactacaat ggtcgattgt
      541 taaggccgat caaacaccca aggaagttgt tgagcaggtt tatgccgtgt taaaggctcg
      601 ttgtcctaag gcttggccgt gttagccaat actttggata acttcaaaaa tataaacttt
      661 gggttataat tagttggagg ttatatttat agacgtaatt accacgtccg tgtaaaaagt
      721 gccatctacc tatcaaatca acaaaaatat cactttcaaa ataacaagga gtcgttgatt
      781 tttgaggtgt aaatagctcg tagtaggttg tgcgtatcgt gcctcgctaa tcaaatcatg
      841 ctaaaattaa tcgaataaat cgcaccactt tttgagtgag ccacccctga tgccacatac
      901 ttttgtcaat ccctttactg attttggctt taaacgcttg tttggcacag agcctaacaa
      961 aggcttattg attagttttt taaatacgtt attgcctgcc aaacatcaaa ttcaagattt
     1021 gcaatacacc aaaaacgaat atcagggtta taccgtatt
//
LOCUS       DAOQMB010000860         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_135125_length_1058_cov_15.408774, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000860 DAOQMB010000000
VERSION     DAOQMB010000860.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135125_length_1058_cov_15.408774"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..228
                     /gene="ccoS"
                     /locus_tag="PLY05_14545"
     CDS             61..228
                     /gene="ccoS"
                     /locus_tag="PLY05_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HNC04560.1"
                     /translation="MDYVYLLVSLSLVLLAGAIWGIIWAVKSDQFEDLTGPAERIILD
                     DKELRRQQNGQ"
     gene            191..940
                     /locus_tag="PLY05_14550"
     CDS             191..940
                     /locus_tag="PLY05_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNC04561.1"
                     /translation="MIKNYADNKMASSLLIVTALAAGFFGSPHCLGMCGGIVSALSFA
                     LHQQQTSQRLYLQSLYHLGRLLAYSLLGLLVGFLGQGLLSPLAGSKWPYLFTSLMMIA
                     FGLYLAGWWRGLDKLERLGAKLWQTMTPLRQRFMPINNSPRALVAGMLWGFLPCGLVY
                     SALALAMTSANPLTSSAVMFAFGLGTLPMMLLAGSTAGALKQKLQQHGWRTANGLLIM
                     AFGIWTLIQTVIIQHPNSHQHHPIPQSQNTP"
     gene            937..>1058
                     /locus_tag="PLY05_14555"
     CDS             937..>1058
                     /locus_tag="PLY05_14555"
                     /inference="COORDINATES: protein motif:HMM:NF021429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HNC04562.1"
                     /translation="MNASKDPLHGLTLQILLERLVYLYGWQELARFININCFKN"
BASE COUNT          269 a          209 c          232 g          348 t
ORIGIN      
        1 atgtcgttaa gttctttatt tgtggtattt aatgccttac gcgtttgaca gaggtgtctt
       61 atggactatg tctatttgct agtatcgtta agtctggtac tgcttgctgg tgcaatttgg
      121 ggcattattt gggcagtaaa aagcgatcaa tttgaagact taactggccc cgctgaacga
      181 attattcttg atgataaaga actacgccga caacaaaatg gccagtagtt tattaatcgt
      241 cactgctctt gcggcaggtt tttttggctc gccacactgt ttgggaatgt gtggtggaat
      301 tgtcagcgca ttgagctttg ccttacatca acaacaaact agccaacgtt tgtatttaca
      361 aagcttgtat catcttggtc ggttattagc ctatagttta ctaggtttgc tcgttggttt
      421 tttaggtcaa ggtttattaa gccctttggc tggctcaaaa tggccttatt tattcacctc
      481 tttaatgatg atagcgtttg gcctatattt ggcaggttgg tggagaggtt tagacaaact
      541 tgaacgtcta ggcgcaaaat tgtggcaaac catgacacct ttgcggcaac gatttatgcc
      601 tatcaataac agccctcgtg cgctagtggc aggcatgtta tggggattct tgccctgtgg
      661 cttggtctat agtgctttag ctttggccat gaccagtgct aatccattaa cctcaagtgc
      721 ggtcatgttt gcgtttggct taggcacttt gcccatgatg ttattggcag gtagtaccgc
      781 aggcgcactt aaacaaaaac tgcaacaaca tggctggcgt actgccaatg gtttgttgat
      841 tatggctttt ggtatttgga cattaattca aacagtcatt attcaacatc caaacagcca
      901 tcaacaccac cctattcctc aaagtcaaaa cacaccatga acgcatctaa agacccatta
      961 catggcttaa cactacaaat tttacttgag cgtttagtgt atttatacgg ttggcaagaa
     1021 ctggctcgtt ttattaacat taattgtttt aagaatga
//
LOCUS       DAOQMB010000861         1058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_135157_length_1058_cov_4.100698, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000861 DAOQMB010000000
VERSION     DAOQMB010000861.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135157_length_1058_cov_4.100698"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLY05_14560"
     CDS             complement(<1..320)
                     /locus_tag="PLY05_14560"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC04563.1"
                     /translation="MSNFIAVYDANVLYPAPLRDLLMRLALEDMYRAKWTNLIHDEWI
                     RGVLRQRPELALELQRTRTLMDAHVRDSLVEEYEYLIPALDLPDADDRHVLAAAIHCH
                     ASVIV"
     gene            complement(324..782)
                     /locus_tag="PLY05_14565"
     CDS             complement(324..782)
                     /locus_tag="PLY05_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004869856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNC04564.1"
                     /translation="MTNNLVTTLPSKAEADMARASSRQLAVHLKTNFETQTFSLKTDS
                     GNQELVEIPTSAVRLFIELLAEMANGNAVHLIPVHAELTTQEAADILKVSRPFVVKLL
                     DSGDIPYHKVGTHRRVLYRDIATYKQRIDQKREHALDELAAQAQNLGMGY"
BASE COUNT          317 a          217 c          186 g          338 t
ORIGIN      
        1 acaataacac ttgcatgaca atgaattgcg gctgctaaaa catgtcgatc atctgcatca
       61 ggtaaatcta aagcaggaat tagatactca tactcttcaa ctaaactatc acgaacatga
      121 gcatccatta gtgttctcgt tctttgtaat tctagggcta actctggccg ttgccttaat
      181 acacctcgaa tccactcatc atgaattaaa tttgtccatt ttgcccgata catatcttct
      241 aatgcgagac gcattaataa atctcttaat ggagcgggat ataaaacatt ggcatcataa
      301 acggcaataa aattactcat ctattagtag cccataccca agttctgtgc ttgggctgcc
      361 aactcatcaa gagcatgctc acgtttttgg tcaatacgtt gtttataagt agcaatatca
      421 cgatataaaa cacgtctatg tgtgcctact ttgtgataag gaatgtctcc tgaatctaat
      481 aatttgacca caaatggacg agacactttt aaaatatctg cggcttcttg tgtggttaat
      541 tcggcatgaa caggtattag gtgtacggca ttgccattag ccatttctgc taacaattca
      601 ataaataaac gaacggcaga ggttggaatt tcgaccaatt cttgattgcc agagtctgtt
      661 tttaagctaa acgtttgagt ttcaaagttt gtttttaaat gaactgctaa ttggcggcta
      721 cttgctcgtg ccatatcagc ctctgcttta ctgggtaaag ttgttactaa gttgttggtc
      781 attgcaatca cattattaag ctagtatgtc atgctattga ctttagcata tccgaaatat
      841 tcgaaacaag cgaaatattc gaaaaatctt aaatcagact atgtttataa aaaaacttac
      901 cccaccacgc ctctcaccgc atttgcccta ccactaatat ttaaacgatt gcatatgtcg
      961 aatgccttat ttttgtaggg tgggttgacg taggaaaccc accttttgag gatgatacat
     1021 tttgtcctta tccattctgt gcctgcttga cataaaac
//
LOCUS       DAOQMB010000862         1056 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_135645_length_1056_cov_15.473526, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000862 DAOQMB010000000
VERSION     DAOQMB010000862.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1056
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135645_length_1056_cov_15.473526"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="PLY05_14570"
     CDS             complement(<1..591)
                     /locus_tag="PLY05_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04565.1"
                     /translation="MNTIYKAESFNSLPCPQVVHVSLGAYGRLTHQQAIKLALDVTPR
                     EPLLGYLSTKRLQICPQNLGQFTPELALQLKAELAHIEFRLHANVHIERQHKIVDISD
                     WPNEKEWFKQMAHLSHLLDAPVYTAHAGKRGTCSVSEVLQFTQEIEDLFGIPVGIEGH
                     YPTPNGKQHWLISTWQEYQQLLESGVNYALDLSHLNI"
     gene            complement(575..1024)
                     /locus_tag="PLY05_14575"
     CDS             complement(575..1024)
                     /locus_tag="PLY05_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04566.1"
                     /translation="MMGYSPKSFEAVHANRQPLLTALAALGITQLVVRYEGGGDSGDV
                     SELEIFPELLAQANIANTLKVEQLTYHCLAGEYQDGEYRYFLQEQQSSIDSALRDFVL
                     TWVDAHHGGWENNDGGSGTMTINVSEGTFRLEHTEYYTECSNYEYDL"
BASE COUNT          319 a          234 c          207 g          296 t
ORIGIN      
        1 aatattcaaa tgcgacaaat ctaaggcgta attcaccccc gattcgagca gttgttgata
       61 ttcttgccat gtactgatta accaatgctg tttaccgttt ggcgtagggt aatgaccttc
      121 aatacccaca ggtatgccaa ataaatcctc aatttcttgg gtaaactgta aaacctccga
      181 gactgaacaa gtaccgcgtt tacctgcatg agcggtataa acaggcgcat ccaacaagtg
      241 cgataaatgc gccatctgtt taaaccactc tttttcattt ggccaatcgc ttatatcgac
      301 gattttatgc tgtctttcga tatggacatt tgcatgaagc ctaaactcaa tgtgagctaa
      361 ttctgctttc aattgcaagg ctaactcagg ggtaaattga cccaaattct gtggacaaat
      421 ttgtaagcgt tttgtggaca aataaccgag taatggctct cgtggtgtaa cgtctaaagc
      481 cagtttaatc gcttgttgat gagttaatcg accataagct cctaaagaca catgcaccac
      541 ttgaggacag ggcagagagt taaaggattc agccttataa atcgtattca taattgctgc
      601 actccgtgta atactcggta tgttctaaac gaaaggttcc ctcagagacg ttaatggtca
      661 tcgtccctga accgccatca ttgttttccc atccgccgtg atgagcatca acccacgtta
      721 gtacaaaatc acgtaaggca gaatcaattg aagattgttg ctcctgtaaa aagtaacgat
      781 attcaccgtc ttgatattcg cctgctaaac agtgataagt cagttgctca actttcaagg
      841 tgtttgcaat attggcttgt gctaataatt caggaaaaat ctctaattca gagacatcac
      901 ccgaatcacc accgccttca taacgcacca ctaattgggt aatacccaag gcagcaaggg
      961 cagtgagtag cggttgacga ttagcatgta ccgcttcaaa ggatttagga ctgtaaccca
     1021 tcatgataaa actccaactt aataacgata attaag
//
LOCUS       DAOQMB010000863         1055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_135924_length_1055_cov_14.499000, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000863 DAOQMB010000000
VERSION     DAOQMB010000863.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135924_length_1055_cov_14.499000"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLY05_14580"
     CDS             complement(<1..663)
                     /locus_tag="PLY05_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HNC04567.1"
                     /translation="MLTIEEMRSYQATKDLLKDKVILVTGASDGIGRVAAKTYAKHGA
                     TVILLGRDLKKLEQVYDEIEADGGAQPAIIPMNFSSAIPTEYEQLAINIEQEFGRLDG
                     ILHSAGILGDLTPLEMYDPNTWDEVMKVNLRAPFMLTQHLLPLLKRAPEASVIFMSSS
                     VGRKARAFWGAYAVAKAGIENLTVLFADELANTSKVRVNCINPQGTRTAMRAKAYPAE
                     DPS"
     gene            complement(666..>1055)
                     /locus_tag="PLY05_14585"
     CDS             complement(666..>1055)
                     /locus_tag="PLY05_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HNC04568.1"
                     /translation="LEGLLTQFEKKQIPWGIVTNKPRHLSEALLSALNLTERCAVLVC
                     PDDVKNTKPDPEPMYLACKKLAVDCQKTIYIGDHLRDIQAGKNANMTTIAVGFGYIVE
                     GEKIEDWQADYSVETVAELVDLFKLGE"
BASE COUNT          298 a          233 c          194 g          330 t
ORIGIN      
        1 gcttgggtct tccgcaggat aggctttggc gcgcattgcc gtacgagtac cttgaggatt
       61 aatacaatta acccgcactt tagaggtgtt agctaattca tcggcaaata acacagttaa
      121 attttcgatg cctgctttag caacggcata cgctccccaa aaagcgcggg ctttgcggcc
      181 aacactgcta ctcataaaaa tcactgatgc ttcgggcgca cgttttaata acggcaacaa
      241 atgttgggtt aacataaatg gcgcgcgcaa gtttaccttc atcacttcgt cccaagtatt
      301 tgggtcatac atttcgagcg gtgttaaatc gcccaaaatg ccagcactgt gtaaaatacc
      361 atctaaacga ccaaattctt gttcaatatt aatggctaat tgctcatatt ctgtgggaat
      421 agcagagcta aagttcattg gaataatggc aggttgtgcg ccaccatccg cctcaatttc
      481 atcataaact tgttctaatt tttttaggtc acggcctaat aaaatcactg ttgcgccatg
      541 tttagcgtaa gtttttgcag ccactcgacc aatgccatca cttgcacctg tcaccaaaat
      601 tactttatct tttaataaat cttttgttgc ttgataagaa cgcatttctt caatggtcag
      661 cataattatt ctcctaattt aaataaatct actaattcgg caacggtttc aacactatag
      721 tcggcttgcc aatcttcaat tttttcaccc tcaacaatat aaccaaagcc aacagcaata
      781 gtggtcatgt tggcattttt gcctgcttgg atatcgcgaa gatgatcgcc tatataaatc
      841 gttttttgac aatcaacagc cagttttttg caagctaaat acatcggttc agggtcaggc
      901 ttagtgtttt tgacatcatc agggcaaacc aatactgcac aacgttctgt taagtttagt
      961 gcagataata aggcttcgct taaatgtcta ggtttgttgg tgacaatacc ccacggaatt
     1021 tgtttttttt caaattgcgt taataaaccc tctaa
//
LOCUS       DAOQMB010000864         1054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_136190_length_1054_cov_11.319319, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000864 DAOQMB010000000
VERSION     DAOQMB010000864.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136190_length_1054_cov_11.319319"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLY05_14590"
     CDS             complement(<1..455)
                     /locus_tag="PLY05_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04569.1"
                     /translation="MNNILEQYEKHWPTTVGKAFLCDRVVLHGQDLHHQLGNWEWFKL
                     YCFSFMRREIPENQLKLLNFFWVATSYPDPSIWPNHIVALAGTTRSTASLTLMAGLSV
                     SEASIYGRRPEKRALDFFYRSQQAIEQGQTLEQVIQAELALGRTIYGYGR"
     gene            complement(452..>1054)
                     /locus_tag="PLY05_14595"
     CDS             complement(452..>1054)
                     /locus_tag="PLY05_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04570.1"
                     /translation="PRDHSIRAAMNGGVGGSTAAGCLMAALAVGAGQYGGAHEVFHAV
                     NIWQFAKQDLARCQQAMEKLLANKDIDIWQPMEHVAGFDPHGVSCPTIILQSLAYLAK
                     LMPNGQLMWLVNHRVELEKTANGTLAMTGLFAAACVDLGLSAEQAEMLFLQLRLSGAA
                     VHALEQQQLGWKKFPYVSQLIDLQKDAPVFPMPNIEDFDL"
BASE COUNT          310 a          259 c          182 g          303 t
ORIGIN      
        1 cgaccataac cataaattgt tcgccctaaa gctaattcag cctgtatcac ttgttcaagt
       61 gtttgcccct gttctatagc ttgttgacta cgataaaaaa agtctaaggc tcgcttttca
      121 ggacggcgtc cataaatact ggcttcggat accgatagtc ctgccattaa tgttaaagat
      181 gcggtgctac gggttgttcc tgctaaagca acaatatgat ttggccaaat actggggtca
      241 ggatagctgg ttgctaccca aaaaaaattt aatagtttga gttgattttc aggaatctct
      301 cggcgcataa aactaaaaca atacagttta aaccactccc aattccctaa ttgatgatgc
      361 aaatcttgac catgtaacac cactctatca cataaaaatg ctttaccaac ggtggttggc
      421 caatgttttt catattgctc taaaatgtta ttcataaatc aaaatcctca atgttaggca
      481 taggaaatac gggcgcatct ttttgtaaat caatcaattg actgacataa ggaaactttt
      541 tccagcctaa ttgttgttgt tctaaggcat gaacagccgc acccgataac cttaattgta
      601 aaaacaacat ttcggcttgt tcggcactca atcctaaatc cacgcaagca gccgcaaaca
      661 accctgtcat tgccaatgtg ccattagctg ttttttctag ctctactcta tgattaacca
      721 accacatcaa ctgtccatta ggcattagtt tggccaaata cgctaagctt tgcaaaataa
      781 tcgttggaca gctaacgcca tgcgggtcaa aacctgcaac gtgttccata ggctgccaaa
      841 tatctatgtc tttattagct aataattttt ccatcgcttg ctgacaacga gctaaatctt
      901 gttttgcaaa ctgccatata ttcacggcat gaaacacttc atgtgcacca ccatactggc
      961 ctgcccccac tgctaacgcc gccattaaac aacctgcggc ggtactacca cctaccccac
     1021 cattcatagc tgctcgaatg ctatggtcac gcgg
//
LOCUS       DAOQMB010000865         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_136455_length_1053_cov_16.675351, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000865 DAOQMB010000000
VERSION     DAOQMB010000865.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136455_length_1053_cov_16.675351"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1053
                     /gene="dnaG"
                     /locus_tag="PLY05_14600"
     CDS             <1..>1053
                     /gene="dnaG"
                     /locus_tag="PLY05_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNC04571.1"
                     /translation="YDILEEAAVFFEQQLQQSPQRDKAQAYLSKRGLDDQTIKHWRVG
                     YAPAGWDNLYKVFADNAEKVKLLQDVGLIVHNEQRDSYYDAMRDRVIFPIRDNRGRVI
                     AFGGRVLDDSKPKYLNSPESPIFHKSEELYGFYESKQAKQTFNKVMVVEGYLDVIAVH
                     QAGFPFAVATLGTATSSTHIERIFKNVPDVIFCFDGDNAGRKAAWRALESALPTLKEG
                     VSAQFLFLPDDHDPDSLIKDRGAESFADILKEGLSLADFLFNQLSTDLRLDSLEGKSR
                     LASLAKPLIEKVPVGVYRQLLWQRLSQLTGLDEQALKAAMPIKESALPAKTEANPQHP
                     AQSHTFAIPRREFIDPS"
BASE COUNT          310 a          216 c          227 g          300 t
ORIGIN      
        1 tatgacattt tagaagaagc ggcggtattt tttgaacagc aactacaaca atcgcctcaa
       61 cgtgacaaag cccaagccta tttaagcaaa cgtggcttag atgaccaaac catcaaacat
      121 tggcgcgttg gatatgcgcc tgctggttgg gacaatctgt ataaagtttt tgccgacaat
      181 gccgaaaaag ttaagctgtt acaagatgtt ggcttgattg ttcataacga acaacgcgac
      241 tcctattatg atgccatgcg tgacagggta atttttccga ttcgcgacaa tcgtggtcga
      301 gtgattgcct ttggtggtcg tgtcttagac gatagcaaac ccaaatatct taactcgccc
      361 gaatcgccta tttttcataa aagtgaagaa ctgtatggct tttatgaaag taaacaagcc
      421 aaacaaactt ttaataaagt gatggtggtt gaaggttatt tagacgtgat tgctgtgcat
      481 caagcaggtt ttccgtttgc cgttgccacc ttaggcacag ccaccagtag cacacacatt
      541 gaacgtattt ttaaaaatgt gcctgatgta attttttgtt ttgatggtga taatgcaggt
      601 cgcaaagcgg catggcgcgc gctagagtct gccttgccaa cgttaaaaga aggcgtatcg
      661 gcacagtttt tatttttgcc tgatgaccat gatccagatt cgttaattaa agaccgtggt
      721 gcagaatcgt ttgccgatat tttaaaagaa ggtttaagtc tggctgattt tttatttaat
      781 cagttaagca ccgatttacg tcttgactct ttagaaggca aaagccgttt agccagttta
      841 gccaaaccct taattgaaaa agtgcctgtg ggtgtttatc ggcaattatt atggcaacga
      901 ttaagtcaat taacaggttt agatgagcaa gccttaaaag cagcgatgcc gattaaagaa
      961 tcagcactac cagccaaaac cgaagctaac ccacaacatc ccgcacaaag tcataccttt
     1021 gccattccca gacgcgaatt tattgacccc agt
//
LOCUS       DAOQMB010000866         1053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_136457_length_1053_cov_12.195391, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000866 DAOQMB010000000
VERSION     DAOQMB010000866.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136457_length_1053_cov_12.195391"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLY05_14605"
     CDS             complement(<1..578)
                     /locus_tag="PLY05_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04572.1"
                     /translation="MLVNNPLKRPHLNDDEKEMFAMKRMAIANALAPYLSQETLEEAL
                     IIWEHKYAHQPTFALQRFISEFCDNNVAGQRSKILQALFLALNNSPNGSAATESEKIA
                     TPPVLTQGATSCFELLLTNILSSLTHDHQQKVRLLTTQQIETLKLNTIITRDLKAFFS
                     QGYTLPSSFYMEVKAMRRCLNLVYVALCDVLGP"
     gene            complement(643..>1053)
                     /locus_tag="PLY05_14610"
     CDS             complement(643..>1053)
                     /locus_tag="PLY05_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010201800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNC04573.1"
                     /translation="MDPQFAIVTGIIAGGVTLVGPTLLAKFRLDDVVDAVSVHGFCGA
                     WGTLAAGLFYAGDLFNGQRVMIQALGIAVAFVWGFGLSWLMYTAIDKMIGLRASPLHE
                     QRGLDYTEHAEIGYPEFQHQKLFNSENLASRGEL"
BASE COUNT          333 a          226 c          192 g          302 t
ORIGIN      
        1 ggccctaaaa catcacacaa ggccacataa accaagttta aacaacgacg catggctttg
       61 acttccatat aaaatgaact tggtaatgtg taaccctgac taaaaaatgc ttttaaatca
      121 cgagtgataa tcgtatttag tttgagcgtc tcaatttgtt gtgtggttag tagccgaact
      181 ttttgttgat gatcatgtgt taaacttgac agaatgttgg ttaacaataa ctcaaaacaa
      241 gacgtcgccc cttgtgttaa gactggaggt gttgctattt tttctgattc tgttgctgca
      301 gaaccatttg gcgagttatt gagtgccaaa aatagagctt gaagaatttt agagcgttgg
      361 cctgcaacat tgttatcaca aaattcacta ataaaacgtt gcagcgcaaa agttggttgg
      421 tgtgcgtatt tatgctccca aataattaac gcttcctcta aagtctcttg acttaaatac
      481 ggtgccaaag cattggcaat ggccattctt ttcatcgcaa acatttcttt ctcatcatca
      541 tttaaatgcg gtcgttttaa cgggttattc actaacatag acagacctcg cgagtttgtt
      601 gttaagtgga ttaacaacaa acatgaagtt tgattgatta gattacaatt caccccgaga
      661 ggctaaattc tcgctgttga ataacttttg atgttgaaat tcgggataac cgatttcggc
      721 atgttcggta tagtccaaac cacgttgttc atgtaatggg ctagcacgta aaccaatcat
      781 tttatcaatt gcggtgtaca tcaaccatga caaaccaaag ccccaaacaa aagcaacagc
      841 aatccctaat gcttgaatca tcacacgttg accattaaac aaatcacctg cataaaacaa
      901 acctgcggct aatgttcccc atgcaccgca aaaaccatgc acgctgacgg catccaccac
      961 atcatccaaa cgaaatttag ctaataacgt agggcctact aaggtaacgc caccagcaat
     1021 aatacctgtc acaatcgcaa attgtggatc cat
//
LOCUS       DAOQMB010000867         1052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_136693_length_1052_cov_9.308927, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000867 DAOQMB010000000
VERSION     DAOQMB010000867.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136693_length_1052_cov_9.308927"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PLY05_14615"
     CDS             <1..737
                     /locus_tag="PLY05_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04574.1"
                     /translation="LTHQQAIQLALEVTPREPLLGYLSTKRLQICPQNLGQFTSDLAL
                     QLKAELAHIEFRLHANVHIERQHKIVDITDWPKEQAWFKQMAHLSHVLDAPVYTAHAG
                     KRGTCSVAEVLQFTQAIEDLFGIPVGIEGHYPTPNGKHHWLISTWAEYQQLLESEVNY
                     ALDLSHLNILAVQTNHVEWSLVHELLSSPHCLEIHLSDNHGYADQHLPLSNPHALPWW
                     WSLLSSANPNATIFSEGRQRVPIDLA"
BASE COUNT          296 a          231 c          211 g          314 t
ORIGIN      
        1 gtttaactca tcaacaagcg attcaattgg ctttagaggt tacaccacga gagcctttac
       61 tcggttatct atcgacaaaa cgtttacaaa tttgtccaca gaatttaggt caatttacct
      121 ctgatttggc gttgcaatta aaagctgaat tagctcacat tgagtttagg cttcacgcca
      181 atgttcatat cgaaagacag cataaaatcg tcgatataac cgattggccg aaagagcaag
      241 catggtttaa acaaatggcg catttatcac acgtgttgga tgcgcctgtt tataccgctc
      301 atgcaggtaa acgcggtact tgttcagtgg cggaggtttt acagtttacc caagcgattg
      361 aggatttatt tggcatacct gtcggtatcg aaggtcacta ccctacgcca aacggtaagc
      421 atcattggtt aatcagtaca tgggcggaat atcaacaatt gttggaatca gaggtgaatt
      481 acgctctaga tttgtcgcat ttgaatattt tggcggtaca gacgaatcat gtggaatgga
      541 gcttagtgca tgaattactc agcagtcccc actgtttaga aattcatctc agcgataatc
      601 acggttatgc cgaccaacat ttaccgctga gtaaccctca tgcgttgccg tggtggtggt
      661 cattactcag ttcggctaat cctaatgcaa cgattttttc ggaagggagg caacgtgtgc
      721 caatcgattt agcttaatac tttcgctgtg attggctgaa atattctttt tacgccatca
      781 acaatcagct tctatccttc attcaacaca attattttca ttcacctaag cacagtctgt
      841 attacaggct gtgctttttt atgtcgaggt ttttatggag catcttcatt cagagcaaaa
      901 tacatcgtct gctaatatgg gtaatcttat tgttagctca tccttagcaa cggtactgag
      961 tcacgaaatc actgattcaa ctctgccaaa caacaccact aaaactcaca ctcacagtgc
     1021 caacgcgatt cgcctcatca ataccaaagg ct
//
LOCUS       DAOQMB010000868         1050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_137288_length_1050_cov_2.880402, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000868 DAOQMB010000000
VERSION     DAOQMB010000868.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137288_length_1050_cov_2.880402"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLY05_14620"
     CDS             <1..460
                     /locus_tag="PLY05_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04575.1"
                     /translation="AKQAEKVAKGDSYPVNERRNALGIALDVRSGATTNLRIRWDDTN
                     GLKAFSDWINAYSQKTIPSRVAYDTRNVYARTRFALIGTQELGIPQAEFKRMLDHART
                     HNGEKLTADNRHLLEQRALCLGYDKLDQLANELIVARWFAAKTAKDLGQQ"
     gene            477..>1050
                     /locus_tag="PLY05_14625"
     CDS             477..>1050
                     /locus_tag="PLY05_14625"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNC04576.1"
                     /translation="MNTYLIEPLAPLVFRSAKPFGSQSGTEGANFPLPSSMAGMLRTV
                     TADQHNMVFSSDLKQQIVKGPFLTRYENKDVFDILVPKPTDAIYLKDSTDKTILIRLS
                     PRPFESGCYSDLPDGLTPVQMEQDLEGKSTMGPQYWTLTDLIAWQEKTIKFEDVQAHG
                     LNNLPCEQRTHVALNDVTLASESGQLFQTAG"
BASE COUNT          339 a          192 c          229 g          290 t
ORIGIN      
        1 agccaaacaa gccgaaaaag tcgctaaagg ggatagttat cctgtaaatg aacgtcgtaa
       61 tgctttagga attgcattag acgtacgttc tggggcaact acaaatttgc gtatacgttg
      121 ggatgatact aatggcttaa aagcatttag tgactggatt aatgcgtata gccaaaaaac
      181 gattcccagt agagtcgcgt atgacacccg taatgtatat gcacgtacca gatttgcatt
      241 aataggtaca caggagcttg gtattcctca agcggaattt aagcgtatgt tagaccatgc
      301 ccgcacacac aatggcgaaa agctcactgc cgataatagg catcttttgg agcaacgcgc
      361 gctctgttta ggttatgaca aactagacca attggcgaat gagttaattg ttgctcgttg
      421 gtttgcagct aaaactgcta aagacttagg acaacaataa agggcagaaa aataccatga
      481 atacatattt gatagagcct ttagcaccat tggtttttag gtctgctaag ccttttggga
      541 gtcaatcagg cacagagggt gctaactttc ctttaccgtc gagtatggca gggatgttgc
      601 gtaccgtaac agctgatcaa cataatatgg tttttagtag cgatttaaaa caacaaattg
      661 tcaaaggccc ttttttaacc cgttatgaaa ataaagatgt ttttgatatt ttagtgccta
      721 agccaacaga tgccatatat ctaaaagatt caacagataa aacgatctta atacgtttaa
      781 gcccaagacc ttttgaaagt ggttgctata gtgatttacc agatggcttg acacccgtac
      841 aaatggagca agatttagaa ggaaaatcta ctatggggcc acaatattgg acattaactg
      901 acttaatagc atggcaagag aaaactatta agtttgaaga tgttcaagcc catggattaa
      961 ataacttgcc gtgtgaacag cgtactcacg ttgcactaaa tgatgtgacg cttgccagtg
     1021 aatcaggtca attatttcaa acggcaggtt
//
LOCUS       DAOQMB010000869         1049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_137471_length_1049_cov_16.865191, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000869 DAOQMB010000000
VERSION     DAOQMB010000869.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137471_length_1049_cov_16.865191"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PLY05_14630"
     CDS             complement(<1..377)
                     /locus_tag="PLY05_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HNC04577.1"
                     /translation="MSKHIVILAGDGIGPEIMAEAVKVLDKVNQQFNLDVTYSHELLG
                     GCAIDATGVPLPEQTLEAAQKADAILLGAVGGPKWDTIERSIRPERGLLKIRSALNLF
                     ANLRPAILYPQLASASSLKPEIVA"
     gene            complement(399..1046)
                     /gene="leuD"
                     /locus_tag="PLY05_14635"
     CDS             complement(399..1046)
                     /gene="leuD"
                     /locus_tag="PLY05_14635"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016542413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNC04578.1"
                     /translation="MQAFTVLTGLVAPLDRANVDTDQIIPKQFLKSIKRIGFGVNLFD
                     EWRYLDEGYPGQDCSKRPINTEFVLNQARYQGAQVLLARQNFGCGSSREHAPWALDEY
                     GFRAVIAPSFADIFFNNCFKNGLLPVVLSEEQVDILFKECEANEGYQLTVDLAKQQVI
                     RPNGEAFSFEVDAFRRHCLLNGLDDIGLTLQAAEDIRAFEEKAKVARPWVFNALR"
BASE COUNT          326 a          240 c          188 g          295 t
ORIGIN      
        1 gcaacaatct caggctttaa gctagaggca gaggctaatt gtggatataa aatcgctggg
       61 cgtaagtttg caaataagtt tagtgcagaa cgaatcttta acaagccacg ttctggacga
      121 atcgaacgct caatggtatc ccacttagga ccaccgactg cacccaataa aatagcatcc
      181 gctttttgcg ctgcttctaa agtttgctca ggtaatggca cacctgtcgc atcaatcgca
      241 caaccgccta atagttcatg ggagtacgtg acatctaagt taaattgttg attgacttta
      301 tctaacactt taacggcttc ggccataatt tctgggccaa taccatcacc cgctaaaata
      361 acaatatgtt tggacatgtt tcttactctt taatctgatt aacgcaaagc attaaatacc
      421 catggacgag ccacttttgc tttttcttca aaggcacgaa tgtcttcggc tgcttgtaaa
      481 gttaaaccaa tatcgtccaa accatttaat aagcaatgac gacggaaagc atctacttca
      541 aagctaaatg cttcgccatt tgggcgaatc acttgttgtt tggctaaatc tactgttaac
      601 tgatagcctt cgttggcttc acattcttta aataaaatat caacttgttc ttcgcttaat
      661 accacaggta ataagccatt tttgaaacag ttgttaaaga aaatgtcagc aaaactcggc
      721 gcaataaccg ctctaaaacc atattcatct aatgcccaag gtgcatgttc acggctagaa
      781 ccacaaccaa aattttggcg tgccaataac acttgtgcgc cttgataacg ggcttggttt
      841 aacacaaact cagtattaat cggacgtttg ctgcaatctt ggccaggata accctcatcc
      901 aaataacgcc attcatcaaa caaattcaca ccaaaaccaa tgcgtttaat cgacttcaaa
      961 aactgcttag gaatgatttg gtcagtatcg acgtttgcgc ggtctaaagg cgcaaccaaa
     1021 ccagttaata cggtaaatgc ttgcataat
//
LOCUS       DAOQMB010000870         1048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_137760_length_1048_cov_17.025176, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000870 DAOQMB010000000
VERSION     DAOQMB010000870.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_137760_length_1048_cov_17.025176"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PLY05_14640"
     CDS             complement(<1..368)
                     /locus_tag="PLY05_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNC04579.1"
                     /translation="MLVLDTNTISYYFRNEPCVVQQLQATPLQQIAVPAIVVYELKYG
                     ILRLPHEAALPRLKALSKFLQFLSIIDVNEKVAELAAQIRVDLEKTGLPIGAHDVLIA
                     ASALSVNGQLVTRNVREFERV"
     gene            complement(368..595)
                     /locus_tag="PLY05_14645"
     CDS             complement(368..595)
                     /locus_tag="PLY05_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04580.1"
                     /translation="MSQITLYLDDEAEQLLRQSAQAAGLSNSKWIAELIKKHARQEWP
                     TELKNLAGAFADFPLRDHNLQHTEDTPRVGF"
     gene            complement(671..>1048)
                     /locus_tag="PLY05_14650"
     CDS             complement(671..>1048)
                     /locus_tag="PLY05_14650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004816044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNC04581.1"
                     /translation="DVGTGQITKICNQMIVASNAVLIAETVALAAKAGVDARQLAPAL
                     AGGFADSLPLQILAPRMATDSFEPVQWKVQTLLKDLDNAVALAKENTSSTPISALASQ
                     LLRLHAATGASLEDLSSVVRLFK"
BASE COUNT          313 a          205 c          205 g          325 t
ORIGIN      
        1 actctttcaa actcacgtac attacgtgtc actaattggc catttaccga taaagcagag
       61 gcggcaatta atacatcatg tgcgcctatt ggtaaacctg ttttttctaa atcgacacga
      121 atctgagcgg ctaattcagc gactttttca ttaacatcaa taatagataa aaattgcaaa
      181 aacttgctta aggctttgag tcgtggcaag gcggcctcat gtggtaaacg caaaatccca
      241 tatttgagtt cataaacaac aatagcgggg acagcaattt gttgcaaagg tgttgcctgt
      301 aattgttgca ccacacaagg ctcatttcta aaataatagc taatggtatt ggtatctaaa
      361 actaacatta aaagcctact cttggtgtat cttctgtgtg ttgtaagtta tggtctcgta
      421 aaggaaaatc cgcaaaagcc cctgctaaat ttttaagttc agtcggccat tcttgacgtg
      481 cgtgtttttt aattaattcg gcgatccatt tgctattaga taagcctgcc gcttgtgctg
      541 attggcgtaa taattgttca gcttcgtcat ccaaatataa agtaatttga gacataaaaa
      601 cctcttataa attatattag ctattatagt gtggcaaaga tataggatca attttattta
      661 aagatcagtt ttatttaaac aatcttacca ccgaactcaa atcttccaaa ctggcgcctg
      721 ttgctgcatg aagtcttaat agttgtgatg ctaaagcaga aatgggtgta ctactggtat
      781 tttctttggc taaggcgaca gcattatcca aatcttttaa taaagtttgc actttccatt
      841 gcacaggctc aaagctatct gttgccatgc gtggtgctaa aatttgtaag ggtaatgagt
      901 cggcaaagcc acctgcaagg gcaggggcga gttggcgagc atcaacacct gctttcgcgg
      961 ctaaagccac cgtttcagca attaaaacag cattactggc gacaatcatt tgattgcaaa
     1021 ttttggtaat ttgccctgtg ccaacatc
//
LOCUS       DAOQMB010000871         1046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_138315_length_1046_cov_15.787084, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000871 DAOQMB010000000
VERSION     DAOQMB010000871.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138315_length_1046_cov_15.787084"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1046
                     /locus_tag="PLY05_14655"
     CDS             <1..>1046
                     /locus_tag="PLY05_14655"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004697764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC04582.1"
                     /translation="HDFSTSAWVLMVVYLYMNGLGITAGYHRLWAHKAYDAHWSVQIV
                     LMLWGTAAIQNSILNWASGHRVHHRFVDDVDKDPYSAKRGFWFSHIGWMLRDYPSGQL
                     DYSNSPDLLKNKIVMFQHNYYLPLVLLMNIAVPMLVGYAIGDFWGGVLLVGLLRLVLS
                     HHFTFFINSLAHMWGTRPYTDENTARDNFWLALLTYGEGYHNFHHIFQYDYRNGVKWW
                     QFDPTKWLIFALSCVGLTSNLKRIPNFTIRKAQLLMQFKRAQEQLEQKQRLLPNVDLE
                     ALKQRVSQEYDAFVLTMNEWGKLKEEWYNEKKEAVMQKWEDAAFKTRFNEIEYRLKMQ
                     SKRMQLLMQQMVQP"
BASE COUNT          306 a          185 c          214 g          341 t
ORIGIN      
        1 accatgattt tagtaccagt gcatgggtac tgatggttgt ttatttgtat atgaatggtt
       61 taggtattac cgctggttat catcgtttat gggcacacaa agcctatgat gcgcattggt
      121 cagtgcaaat tgtattaatg ttgtggggaa cagcagctat ccaaaatagt attttgaatt
      181 gggcttcggg tcatcgtgtt catcaccgtt ttgttgatga tgttgacaaa gacccctatt
      241 cggcaaaacg cggcttttgg ttttctcata ttggttggat gttgcgtgac tatccttcgg
      301 gtcaacttga ttacagcaac agtccagatt tactaaaaaa taaaattgtc atgttccaac
      361 acaactacta tttaccttta gtattgttaa tgaacattgc tgtccctatg ttagtgggtt
      421 atgccattgg tgatttttgg ggtggtgtat tattagttgg tttattacgt ttagtgttaa
      481 gccatcactt caccttcttt attaactcct tggcgcatat gtggggtact cgcccttaca
      541 ccgacgaaaa tacagcgcgt gacaactttt ggttagcttt gttaacttat ggtgagggct
      601 atcataactt ccatcatatt ttccaatacg attatcgtaa tggtgttaaa tggtggcaat
      661 ttgacccaac aaaatggtta atctttgctt tatcttgtgt cggtttgact agcaacttaa
      721 aacgcattcc caactttacc attcgtaaag cacaattatt aatgcaattt aaacgtgcac
      781 aagaacagtt agagcaaaaa caacgtttat taccgaatgt tgatcttgaa gccttaaaac
      841 agcgcgtttc tcaagaatat gatgcttttg tactgaccat gaatgaatgg ggtaaactca
      901 aagaagaatg gtataacgag aaaaaagaag ccgttatgca aaaatgggaa gatgcagcct
      961 ttaaaacccg cttcaatgaa attgaatatc gcttaaaaat gcaaagcaag cgtatgcagt
     1021 tattgatgca acaaatggta caaccc
//
LOCUS       DAOQMB010000872         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_138577_length_1045_cov_20.060606, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000872 DAOQMB010000000
VERSION     DAOQMB010000872.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138577_length_1045_cov_20.060606"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(283..>1045)
                     /locus_tag="PLY05_14660"
     CDS             complement(283..>1045)
                     /locus_tag="PLY05_14660"
                     /inference="COORDINATES: protein motif:HMM:NF006123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNC04583.1"
                     /translation="IMNNILITGAASGIGKATAIFFKRKGWQVGLLDIDSKALADLSA
                     QLEGAWYSAVDVTDYTQVQQAISDFAALHDSQLRVLFNCAGVLHMGHFEQLTPEQHHK
                     TLAININGLVNVIHAAFPFLRHTENAQVINMSSAAALYGAPHMASFSAAKFAVRGLTE
                     ALNLEWREHEIWVSDLMPAFVNTPLVHDKDFVAPIVERLGVDLIAEDVALAAWQAVST
                     YRVHHPVGLSFKALMLVNKFAPAKITRRMMAFLSR"
BASE COUNT          329 a          238 c          208 g          270 t
ORIGIN      
        1 ctctgcgtac tcgcaaaaaa caagcagtaa tctgatatta agccaatagg ttttcatatt
       61 caaggcctta ttctgtaagt gacactttcc aacttatcaa ccttgctgat ttgtgccaaa
      121 tcgcaagggg ttgataagcc gtcgtgtcac cgccttatat cctcggccta aaggcgcgag
      181 gttttacggc gagtttgata aagcttgacg taattcggct gagataccac taaaccaact
      241 gccctgcaat aataatggtg catagcgaga agcattcatc atttaacgac ttaaaaaagc
      301 catcatgcga cgggtaattt tggcaggggc aaatttattg actaacatta aggctttaaa
      361 agacaaacca acagggtgat ggacacgata agtacttacg gcttgccatg ctgccaaagc
      421 aacatcttca gcaattaaat ctacgcccaa acgctcaacg atcggggcaa caaaatcttt
      481 atcatggacg agtggcgtat taacaaaggc aggcatcaaa tcactgaccc aaatttcgtg
      541 ctcacgccat tctaaattta aggcttcggt taaaccacgt acggcaaatt tagccgccga
      601 aaaactggcc atgtgtggtg cgccatataa cgcggccgca gagctcatat taatcacttg
      661 ggcattttcg gtgtgacgca aaaagggaaa ggcggcatga atgacattca ccaaaccatt
      721 aatattaatg gctaaggttt tatgatgttg ttcgggcgtg agttgctcaa aatgccccat
      781 gtgcaacaca cctgcacaat taaataacac gcgcaattga ctatcatgca gagcagcaaa
      841 atcactaata gcttgttgaa cttgagtata atcagtgaca tcaaccgctg aataccatgc
      901 tccctcaagc tgcgcagata agtcagcaag tgctttgcta tcaatatcta ataaaccaac
      961 ctgccaacct ttacgtttaa aaaaaatagc cgttgcttta ccaatgcctg atgctgcacc
     1021 tgtgattaaa atattattca ttatt
//
LOCUS       DAOQMB010000873         1045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_138608_length_1045_cov_4.227273, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000873 DAOQMB010000000
VERSION     DAOQMB010000873.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138608_length_1045_cov_4.227273"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..575)
                     /gene="cas2"
                     /locus_tag="PLY05_14665"
     CDS             complement(291..575)
                     /gene="cas2"
                     /locus_tag="PLY05_14665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNC04584.1"
                     /translation="MPQRQLFLAAYDVREPKRLKKALHVLRDYTCGGQKSVFECYLTI
                     AERQRLISRVLGVIDESEDFFLIVPLRQSSPVKTIGTATPPADDNFLYIG"
     gene            complement(741..>1045)
                     /locus_tag="PLY05_14670"
     CDS             complement(741..>1045)
                     /locus_tag="PLY05_14670"
                     /inference="COORDINATES: protein motif:HMM:NF020571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1887 family CARF protein"
                     /protein_id="HNC04585.1"
                     /translation="QGKVTFNELDVMALYGTNLLIAECKTINWDNNKDASPLESIYKL
                     AALADIGGLNTHAVFVSLYELPDNAKTRAAENNIIVIDGKKLLDLPALIKQGINNN"
BASE COUNT          303 a          186 c          214 g          342 t
ORIGIN      
        1 ttggtctagc accttgcaat gtttgtcttt tatgcgtaat ttaagtgttt tcatggcatc
       61 attttaagtg ttagttgtcg ctaaaacaaa tactttctca agaccttatt ctgtatttcg
      121 gcttcgctca ataaccctaa gtgacacttg ccaacttatc aaccttgctg atttgtgcca
      181 aatcgcaagg cgttgataag ccgtcgtgtc accgccttat atcctcggcc taaaggcgcg
      241 aggttttacg gcgaagttga taaatgcttt ccataaacac ctctaagcaa ttaaccaata
      301 tataaaaaat tatcgtcagc agggggagtg gctgtgccaa tggttttaac aggtgagctt
      361 tggcgtaatg gcacaatcaa aaagaaatct tcactttcat caatcacacc taatactcga
      421 ctgattaatc gttgacgttc ggcaatagtt aaataacatt caaaaactga tttttgacca
      481 ccacaggtat aatcacgcaa aacgtgtaat gcttttttta ggcgtttggg ttcacgcaca
      541 tcataggcag ctaaaaataa ttggcgttgt ggcatgacgg catactccaa ttaacaaaca
      601 tcagtctttt ggtgggtatg tggtgagtgt agagcgtttt ttagacgatt gtgtcgttta
      661 taagcttgcg aagtttgttg gttttgagtg agatgaggag aggaggtagc cccatttgtt
      721 gtggggctag gtgaggggat ttagttatta ttaatacctt gtttaataag ggcgggcaag
      781 tctaggagtt tttttccatc aataacaata atattatttt cagcagcacg tgttttggca
      841 ttatcaggta attcatataa tgaaacaaaa acggcatgag tatttaaacc gccaatatcc
      901 gctaatgctg ctaatttata aatagattca agtggtgaag cgtctttatt attatcccaa
      961 ttgatggttt tacattctgc aattagtaag ttagtaccat ataaagccat aacatctaat
     1021 tcattaaagg taactttgcc ttgac
//
LOCUS       DAOQMB010000874         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_138837_length_1044_cov_18.202224, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000874 DAOQMB010000000
VERSION     DAOQMB010000874.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138837_length_1044_cov_18.202224"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..>1044
                     /gene="gshA"
                     /locus_tag="PLY05_14675"
     CDS             29..>1044
                     /gene="gshA"
                     /locus_tag="PLY05_14675"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HNC04586.1"
                     /translation="MNAQLQQQLAWLSPQATVLKNMRRGLEKEALRMSKDGMISQTEH
                     PRALGSALTHPHITTDYSEALIELITPATKSIDETLDFLEDLHRFCYQNMGEERLWLN
                     SMPCMIGLEDSQIPLAYYGESNIGKLKTLYRHGLGVRYGRKMQTIAGIHYNLSFPSEF
                     WQAWQQASQNNQDPQEFINEKYFALIRNFQRHSFLLLYLLGASPAVCACFLLGRQHGL
                     ASLSAGTLYQPYATSLRMSRLGYQNTVQRDLQVSYNHLPDYIDNLSNAIHTPYPPFAD
                     IGVKKDGVYQQMNANVLQIENEYYGLIRPKQTTQRGEKPTQALKARGIEYIEMRCVDL
                     NPFSP"
BASE COUNT          319 a          245 c          193 g          287 t
ORIGIN      
        1 aatttgtctt taaaaataga gaaaacccat gaacgcacaa ctgcaacaac aacttgcttg
       61 gttaagccct caagccaccg tgttaaaaaa tatgcgtcgg ggtttagaaa aagaagcctt
      121 acgcatgagc aaagatggca tgattagcca aaccgagcat ccgcgtgctt taggctcagc
      181 attaacccat ccacacatca ccaccgatta ttccgaagcc ttaatcgagc tgattacccc
      241 tgccaccaaa agcattgatg aaacgttgga ctttttagaa gacctacatc gcttttgtta
      301 tcaaaatatg ggcgaagaac gcttatggct taactctatg ccctgtatga ttggtttaga
      361 agatagccaa attccactgg catattatgg cgaatccaat attggcaaac tcaaaacctt
      421 gtatcgtcat ggcttgggcg tgcgttatgg ccgcaaaatg caaaccattg caggcattca
      481 ctataacttg tcattcccta gcgaattttg gcaagcatgg caacaagcca gccaaaacaa
      541 ccaagaccct caagaattta ttaacgaaaa atattttgcc ttaattcgta attttcaacg
      601 tcatagcttt ttattattgt atttattagg tgcaagtcct gcggtgtgtg cctgtttctt
      661 attaggccgt cagcatggct tagccagttt aagtgcaggc acgctctatc aaccttatgc
      721 cacttcttta cgcatgagcc gtttgggtta tcaaaatacc gtacaacgcg acttacaagt
      781 atcttataac catctacctg attatattga caatttaagc aatgctattc acacgcccta
      841 tccaccgttt gccgatattg gcgtgaaaaa agacggtgtt tatcagcaaa tgaatgctaa
      901 tgtcttgcaa atcgaaaatg aatattatgg tttaattcgc cccaaacaaa ccactcaacg
      961 tggcgaaaaa cccacccaag ctctcaaagc gcgtggtatt gaatacatcg aaatgcgctg
     1021 tgttgacctc aatccattta gccc
//
LOCUS       DAOQMB010000875         1044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_138869_length_1044_cov_4.177958, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000875 DAOQMB010000000
VERSION     DAOQMB010000875.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_138869_length_1044_cov_4.177958"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..679)
                     /locus_tag="PLY05_14680"
     CDS             complement(2..679)
                     /locus_tag="PLY05_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04587.1"
                     /translation="MIIEYFFDFDDKKQLHFQVDLDRQFDFKQDLTAAPEWTKLDNNQ
                     CANCPLKKNVFSHCPAALDLDKVIGEFQQIPAITKVKVTVITPEREYAKRVTLEEGVR
                     ALMGLIMATSACPVFNTLRPNARHHLPFASREESVLRSASLYLMNQYLIAQEGGEPDW
                     ELKGLIKTNEQLQLVNHAFWQRTMTAFQSDANSKALLGFFTLSSNMSASLQSQLVKMK
                     NVFYSGQ"
     gene            complement(800..>1044)
                     /locus_tag="PLY05_14685"
     CDS             complement(800..>1044)
                     /locus_tag="PLY05_14685"
                     /inference="COORDINATES: protein motif:HMM:NF015438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HNC04588.1"
                     /translation="WQTLKIGELELDLVKPCSRCVIPSIDPDTTAKDSSINKVLAEHR
                     RQADGKVYLGQNAIIRHLPKDFVIQQGQAVEIVYKK"
BASE COUNT          326 a          196 c          184 g          338 t
ORIGIN      
        1 gttattggcc actatagaac acattcttca tttttaccaa ttgactttgg agtgaagcag
       61 acatatttga cgataaggta aaaaatccta acaaggcttt agagttagca tcactttgaa
      121 aagcagtcat ggtgcgttgc caaaaagcat gattaactaa ctgcaactgt tcgttagttt
      181 tgattaaacc ttttaattcc caatcaggtt ctccgccttc ttgagcaatt aaatattgat
      241 tcattaaata caatgatgcc gaacgtaata cggactcttc gcggctagca aagggcaaat
      301 gatggcgggc attgggtctt aaagtattaa acacaggaca cgcactggtg gccataatta
      361 agcccattaa ggctctcacg ccttcttcta aggtcactcg tttggcatat tcacgctcag
      421 gagtgatgac agtgactttt actttggtaa tcgcaggaat ttgttgaaac tcgcctatca
      481 ctttgtctaa gtctaaggct gcagggcaat gactaaagac attttttttc aaaggacagt
      541 tagcacattg attattatcc aattttgtcc attcgggtgc ggcagttaaa tcttgtttaa
      601 aatcaaattg acgatctaaa tcgacttgaa aatgtaattg ttttttatca tcaaaatcaa
      661 aaaaatactc aataatcatt gcttgtaact cttattaaaa tttatatttt tagtgtgata
      721 agactaaaat attcatccgt tttaagcaag attatcctca acagacttga gcattggatt
      781 gtagtcaata agtaagtcat cattttttat aaacaatctc aacagcttgt ccttgttgaa
      841 tgacaaaatc tttgggtaaa tgacgaataa tcgcattttg accaagatat actttgccat
      901 ccgcttggcg acgatgctcg gctaatactt tattaattga actatcttta gctgttgtat
      961 caggatcaat actcggaatc acacaacgcg aacaaggttt gactaagtct aattccaact
     1021 caccaatttt cagcgtttgc catg
//
LOCUS       DAOQMB010000876         1043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_139302_length_1043_cov_2.286437, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000876 DAOQMB010000000
VERSION     DAOQMB010000876.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139302_length_1043_cov_2.286437"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLY05_14690"
     CDS             complement(<1..564)
                     /locus_tag="PLY05_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04589.1"
                     /translation="MEKQLIISLLNIYHSEDMRFNQSKSLLAFCQEYNLGRLDRKSLY
                     FSVRDKQDIAALLRYEGIEASTTYAESFKEKTRSQTTQQANNEKLNTANVRLHRVAIK
                     TFYNKPLLWQHQAANFLPLQAHLEIDYQETAHLLAHQSIIVVENWESFNQIDDMALDF
                     SDAGDNPLIVWRGEKEGVRADNALALLK"
     gene            complement(549..>1043)
                     /locus_tag="PLY05_14695"
     CDS             complement(549..>1043)
                     /locus_tag="PLY05_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04590.1"
                     /translation="VPDLSELDNAPSWIQRFSGFDLQSYANLNQASMCEQLADVASRI
                     PAAKIVLQQKQQQGTLTVDEPLAIVELKPSISQLALRGFWQQALKSHQPQSALRWKHQ
                     QIDLKELNDSVWLMVLLHSLQVDSSQKNHPLQQLKTQRCERTANHLWAGNIIVEDILA
                     WKNS"
BASE COUNT          292 a          218 c          193 g          340 t
ORIGIN      
        1 tttgagcaac gctaaagcat tatcggcacg cacgccttct ttttcgccac gccacacaat
       61 taaaggatta tcacctgcat cactaaaatc taatgccatg tcatcaattt gattaaaact
      121 ttcccaattt tcaacaacga ttatgctttg atgagccaat aaatgcgcgg tttcttgata
      181 gtctatttcg aggtgggctt gtaagggtaa aaaattagca gcttgatgtt gccataatag
      241 cggtttgtta taaaatgttt ttatggccac acgatgcaaa cgcacgttgg ctgtatttag
      301 cttctcatta ttggcttgtt gggtggtttg ggaacgtgtt ttttctttaa aactttcggc
      361 atacgttgtg ctggcttcta tcccttcata acgtaataaa gcagcaatgt cttgtttgtc
      421 cctgacacta aaatacagac tttttcggtc taaacgcccc aaattatatt cctgacaaaa
      481 tgctaagagg cttttactct gattaaaacg catatcttca ctatgataaa tatttaataa
      541 gctgataatt aactgttttt ccatgccaaa atatcctcaa caataatatt tcctgcccac
      601 agatgatttg ctgtacgctc gcagcgttgc gtttttaatt gctgaagcgg atgatttttt
      661 tgactgctat cgacctgtaa actatgtaat aagaccatta accacacaga gtcgtttagc
      721 tcttttaggt caatttgttg atgtttccaa cgtaaagccg attgcggctg atggctttta
      781 agtgcttgtt gccaaaagcc tcgtaaagct aactgactga tactcggttt taactcaaca
      841 atcgctaaag gctcatcaac ggttaatgtg ccttgttgct gtttttgttg caacacgatt
      901 ttagccgcag gaatacgact ggcaacatcg gccaattgtt cacacatact cgcttgattc
      961 aaattggcat aactttgcaa atcaaagccc gaaaaacgtt gaatccatga gggcgcattg
     1021 tctaattccg ataaatcagg cac
//
LOCUS       DAOQMB010000877         1041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_139672_length_1041_cov_8.410751, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000877 DAOQMB010000000
VERSION     DAOQMB010000877.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1041
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_139672_length_1041_cov_8.410751"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /gene="repM"
                     /locus_tag="PLY05_14700"
     CDS             complement(<1..841)
                     /gene="repM"
                     /locus_tag="PLY05_14700"
                     /inference="COORDINATES: protein motif:HMM:NF038290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="HNC04591.1"
                     /translation="MGSIFMGLVVKSNELIRASYTLSLVEQRIILMSIVAARDISEIT
                     PDTLCRIKVNEYAELYDVTPQAAYLSLKEAAEVMFDRRVTIQVYDDKLQKKVPLAVRW
                     LSGMNYLPEDGIVTVRFSKDLIEHITEIYENFTRYKIENIAKLNSQYAVRLYELLASW
                     LSARKTPIFSLEEFRSQLGIQPHEYERIQHFKERVLDFALAQINENTDITATYAQHKD
                     GRTITGFSFTIKAKKKKAEPKKSVEPKKAAKEQQIDWVTPPINDEAQKIADTFLALDI
                     EKQA"
BASE COUNT          276 a          204 c          207 g          354 t
ORIGIN      
        1 aggcttgctt ctcaatatcg agggctaaaa aggtatcagc tatcttttgt gcttcgtcat
       61 tgataggcgg tgttacccag tctatttgtt gctctttggc tgcttttttg ggttcgactg
      121 attttttagg ctcggctttt ttcttcttgg ctttaatggt gaatgagaaa cctgtgattg
      181 ttcgaccgtc tttgtgttgg gcgtaagttg ctgttatatc ggtgttttcg ttaatttgtg
      241 ctaaggcaaa atcgagtaca cgctctttaa agtgctggat tcgttcatat tcatgaggtt
      301 gtatacctaa ttggcttcta aattcttcta atgaaaaaat gggggttttt cgtgccgata
      361 gccagcttgc caatagctca tataggcgaa cagcatattg actattcagc ttggcaatat
      421 tttcgatttt gtagcgagta aagttctcat aaatctcggt aatatgctcg attaaatcct
      481 tactaaaacg aaccgtaact attccatctt ctggcaagta attcattccc gatagccaac
      541 gaaccgctaa gggaactttt ttttgcaact tgtcatcata aacttgaata gtgacacgcc
      601 tatcaaacat cacttctgcc gcctctttaa gcgataaata agcagcttga ggggtcacat
      661 cataaagctc ggcgtattca ttgactttaa tacggcaaag cgtatcgggg gttatctcgc
      721 tgatgtctcg tgcagcgaca atcgacatta aaataatgcg ctgctcaacc aagctcagtg
      781 tgtaactggc tctgattaac tcattggatt tcacgactag tcccataaaa atactaccca
      841 tattgagaaa atccttttca accatgtttt gattttaggt atttttacca taaaaaatat
      901 attgggtcaa tttttcaagt aaacgtggct ttatattcaa gtaaacgtgg ctttatattc
      961 aagtaaacgt ggctttatat tcaagtaaac gtggctatat gacctctgta tcccttgcgc
     1021 cacaaggctt acagaggact t
//
LOCUS       DAOQMB010000878         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140260_length_1039_cov_5.991870, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000878 DAOQMB010000000
VERSION     DAOQMB010000878.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140260_length_1039_cov_5.991870"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..965
                     /locus_tag="PLY05_14705"
     CDS             66..965
                     /locus_tag="PLY05_14705"
                     /inference="COORDINATES: protein motif:HMM:NF037521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HNC04592.1"
                     /translation="MSMSKLQQSVTDRIIAKIEEGGLAAWMKPWVDIANKDKAMPYNF
                     KTGNEYQGINILTLLIGAFENGYSHNCWLTFDQALSLGGRVRKGEKSQHCIRYGVYEK
                     EDDHNDKKSYAFVKPFYLFNIEQIDGIEIPQLPDTPIRTIDDSILAIESLVKEECDVT
                     NLGYRIGGNRAFYAPALDLIKMPQGEFNCCSNYAATLAHELIHATGHSKRTNRHHLFK
                     DKFKDDTKASYAFEELVAELGAAMLCAEHGIVGEHLQHESYIDSWFNVLKREPSMLLK
                     AASHASKAHHYLMRRDIEVDIAA"
BASE COUNT          318 a          186 c          222 g          313 t
ORIGIN      
        1 aattacgacg atctcactaa tcgtcgtcgc ttcccactgc gttatgtgtg gctttggaat
       61 tatttatgtc tatgtcaaag ttacagcaat cagtaaccga ccgcattatt gcaaaaattg
      121 aagaaggtgg ccttgccgct tggatgaaac cttgggttga tattgccaat aaggataagg
      181 ccatgcctta taactttaaa acaggtaatg aataccaagg tatcaacatc ttaaccttat
      241 tgattggtgc atttgagaac ggttactctc ataactgttg gcttactttt gatcaggctt
      301 tatcgttagg tggacgtgtg cgtaaaggcg agaaatcgca acattgcatt cgttatggag
      361 tttacgaaaa agaggatgac cataatgaca aaaagtctta tgcgttcgtt aagccttttt
      421 atctattcaa tattgaacag atagatggca tcgaaattcc ccagctaccc gataccccga
      481 tcagaactat tgatgactca attttggcca ttgaatctct tgttaaagag gagtgtgatg
      541 tgactaatct gggttatcga attggtggta atcgtgcttt ttacgctcct gcacttgatc
      601 ttatcaagat gcctcaagga gagtttaatt gttgtagtaa ttatgctgca accttagcac
      661 atgagctgat tcatgcgact ggccattcaa aacgtacaaa tcgccatcat ctctttaaag
      721 ataaatttaa agatgataca aaggcgagtt atgcgtttga ggagttagta gcagagttag
      781 gcgcggcaat gttatgcgct gagcatggta ttgtaggcga gcatctacaa catgaatcat
      841 atattgactc atggtttaat gtgctaaaac gagaaccgtc aatgcttcta aaagcagcaa
      901 gtcacgcatc taaagcgcat cattatttga tgcgtagaga tattgaagtt gatatagcag
      961 cataaaaaca aaagcccttc ttcggagtaa tgccagtcag ttaagcaaat taatttaaaa
     1021 gatgtttaaa atgggtatc
//
LOCUS       DAOQMB010000879         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140273_length_1039_cov_4.370935, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000879 DAOQMB010000000
VERSION     DAOQMB010000879.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140273_length_1039_cov_4.370935"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1039
                     /locus_tag="PLY05_14710"
     CDS             <1..>1039
                     /locus_tag="PLY05_14710"
                     /inference="COORDINATES: protein motif:HMM:NF037658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04593.1"
                     /translation="IIKLLSVSAVTNGRTEVDLSDVLLLKDCLWNHYDNALKVREMVL
                     GVLQGFSYEVVIEGTSQVIEKPKQTSKNVIKGYKGSGTQSDPILIGTYQDIAGLERAD
                     VGQKGYYFKQTADIDCSALTTWPNIDFRGHYDGGGNIIKYGKTGYSLFNSMQSRSSVI
                     NLYLEQLLLASKANDSEILACTTVRASLLGTAQNCYVIACKAERHLIVNNATQCVIKR
                     CHSWWSLIGNTAIASSINNCSVTLKDTRWVNCCSGGIVYLLMQGSIENCYVTGNLTKN
                     TLYNSHFSAIAFICDGTVIRSSALGKLHLKDIQLTGRIVNNIKGQTDIQNNIALDLHQ
                     GQSDAQGKDGRS"
BASE COUNT          325 a          141 c          250 g          323 t
ORIGIN      
        1 agattattaa gttgttgagt gtgtcagcgg tgacgaatgg ccgtactgag gtggatttgt
       61 cggatgtgct gttgcttaaa gattgtttgt ggaatcatta tgataatgcg ttgaaagtga
      121 gggagatggt tttgggggtg ttgcaagggt ttagttatga ggttgttata gagggaacgt
      181 cacaagttat tgaaaagcct aaacagacat ctaagaatgt gattaaaggc tataaaggca
      241 gtggcacgca aagtgaccct attttaattg gcacttatca agatattgca ggtttggaac
      301 gtgcagatgt ggggcaaaaa ggttattact ttaagcaaac ggcggatatt gattgttcag
      361 cactgacgac ttggccaaac attgatttta gagggcatta tgatggaggt ggtaatatta
      421 ttaaatatgg taagacaggc tatagtttat ttaatagcat gcagtcaagg tctagtgtca
      481 taaacttata tttagaacag ttattattgg cttcaaaagc taacgatagc gaaattttag
      541 cttgcacaac ggtacgcgct tcattacttg gaactgcgca aaattgttat gtcatcgctt
      601 gcaaggcaga gcgacacttg attgtaaata atgcaactca gtgtgtaatt aaacgctgcc
      661 attcatggtg gtcgttgatt ggtaacacag caattgcaag tagtataaat aactgctcgg
      721 tgacattaaa agatacaaga tgggtaaatt gttgtagtgg tggtattgta tatttattga
      781 tgcaaggcag tattgagaat tgctatgtta caggtaattt aacgaagaat acgttatata
      841 atagccattt ttctgctata gcttttattt gtgatggaac tgttattcgc agttctgctt
      901 tgggcaagtt acatttaaaa gatattcaac taacaggccg tattgttaat aatataaaag
      961 gccaaacaga tatacaaaat aatattgccc ttgacttaca tcaagggcaa tccgatgcac
     1021 aaggtaaaga cggcagatc
//
LOCUS       DAOQMB010000880         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140281_length_1039_cov_3.984756, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000880 DAOQMB010000000
VERSION     DAOQMB010000880.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140281_length_1039_cov_3.984756"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..130)
                     /gene="dcd"
                     /locus_tag="PLY05_14715"
                     /pseudo
     CDS             complement(1..130)
                     /gene="dcd"
                     /locus_tag="PLY05_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985727.1"
                     /note="Catalyzes the formation of dUTP from dCTP in
                     thymidylate biosynthesis; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
     gene            complement(149..307)
                     /locus_tag="PLY05_14720"
                     /pseudo
     CDS             complement(149..307)
                     /locus_tag="PLY05_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          277 a          235 c          207 g          320 t
ORIGIN      
        1 gtgccacgcc ttcattagcg taaattttgg caggtaaatt cgtggtatta gaaaattcta
       61 aggtcacatg accttcccac tctggctcta aaggagtcac attcactatt attccgcaat
      121 tttgtacaaa aacaccattt tctaaagcaa aattacctgc ttctggtaca gttaaacaat
      181 atacatcatg agtgccagca acccctttaa ctgccacaac tttatgattg tgtggcgctt
      241 gtgtgcctct aaattgactc aaaacatcag gaaaacgtct aaacacacta cggtcacagt
      301 ctaacattct tgctgcggca cgaattgaac ccgtattatg taatgcttgt tttacactat
      361 ccgcattaat ttcatggcga atattgatgt tacgagcggt ctctgcttgt cgttggcgac
      421 gtgcttcggt tgcggcttgc caagcaatac gcactctttc gccttgttta gcacgttctg
      481 tcgcatcctg ataacgctgc tgcattaact cacggtgaat ttggcgagtt tgctcagttg
      541 gattttggcg tttagctaat aattcagcac gagtttgtgc gtatttatct tcatgccaaa
      601 attgtttggc gcgtgtactt tgtgcttgcg agtattctgc tgcccatgct gggtcttgca
      661 tacgcaaata taatgcctct ctaatagccg cactatgttc atcaggatca aaatcatcac
      721 cataattaat atcgttatgt agtttgatgt gttcgttggc atccatacgc tgaatgttcc
      781 acggattatt atttaatcta tcaaaatcaa catggtgacg gtgactattg gccatttcgc
      841 tataaatgcc attgcgtagg ttccattcat cggctaaacg atgggttggg tataaatggc
      901 catttaaagg ctgataggcc atttcataac ctcttaccaa ttgacggtac aaaggcatta
      961 acgaatcacc tgcacgcaac tcacctgcgg cacgccaacg gccatcacgt aacataaata
     1021 aatggtcagg agtagcaaa
//
LOCUS       DAOQMB010000881         1039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140350_length_1039_cov_2.804878, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000881 DAOQMB010000000
VERSION     DAOQMB010000881.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140350_length_1039_cov_2.804878"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..>1039)
                     /locus_tag="PLY05_14725"
     CDS             complement(113..>1039)
                     /locus_tag="PLY05_14725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04594.1"
                     /translation="VSMQLLYGLYSAPSSEFLREDYSKLKLKTQEDVTTRLQWFEDGF
                     LKALAIAENIKGLDRLLNGDVNERFKSNIQGRGVYTPRCLVVARLANNPRFEELATSL
                     ATPRSPGANQESLPTEWPKLVQYLREDINPATCWQQFAQLAKEEQRLEAARLNALQRQ
                     YNPQTSEELMSLASQFYDAKTNLTWQRCCVGQQWVDGKVTGNPQLLSWDEVQKILAQE
                     SDKGWRLPTFEEFKTLIITTRIGCIIKEGFDFYEIQERIFADHWGMRLEDICTIFVKN
                     IENPTIYDAPTFRPAKNNTNLKGVLRLVKSAS"
BASE COUNT          279 a          210 c          195 g          355 t
ORIGIN      
        1 ttaatattaa aggcttgcaa aagctgagtt ttggctcagc ttgcttctta ggctagaagg
       61 ataattttct cttttattta gatgggtttc attcctcaac ccatcataca cactaagaag
      121 ctgacttcac taagcgtaaa acgcctttaa ggtttgtatt attttttgca ggtctaaagg
      181 ttggcgcatc atagattgtt ggattttcaa tattttttac aaatatagtg caaatatctt
      241 ctaggcgcat accccaatgg tcggcaaaaa ttcgttcttg tatttcataa aaatcaaaac
      301 cctctttgat gatacagcca atacgtgttg taataatcag ggttttaaac tcctcaaacg
      361 tcggtaaacg ccaaccttta tctgactctt gtgctaatat tttttgcact tcatcccaac
      421 tgagtaattg cggattgcct gtgactttac catccaccca ttgttggcca acacaacatc
      481 gctgccatgt taaatttgtt ttagcatcat aaaattggct tgctaagctc attagctctt
      541 cagatgtttg agggttatat tgtcgttgta aggcattaag tcttgccgct tcaaggcgtt
      601 gttcttcttt tgctaattgt gcaaactgtt gccaacaggt tgctggatta atatcttcac
      661 gaaggtattg caccagtttt ggccattctg taggtagaga ttcttgatta gcaccaggtg
      721 atcttggcgt agctaagcta gtagctaact cttcaaagcg aggattattg gctaaacgag
      781 ccacgactaa acagcgaggt gtatatacgc ctctaccttg tatgttcgat ttaaatcgtt
      841 cattgacatc cccattaagt agcctatcta aaccttttat gttttcggca atcgctaaag
      901 cctttaaaaa accatcttca aaccattgca gccttgttgt tacatcttct tgggtcttga
      961 gttttagttt gctatagtct tctctcagaa attcagatga gggggcagaa tataggccat
     1021 atagaagctg catgctaac
//
LOCUS       DAOQMB010000882         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140545_length_1038_cov_7.635809, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000882 DAOQMB010000000
VERSION     DAOQMB010000882.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140545_length_1038_cov_7.635809"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..1038)
                     /locus_tag="PLY05_14730"
                     /pseudo
     CDS             complement(69..1038)
                     /locus_tag="PLY05_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612102.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          296 a          246 c          193 g          303 t
ORIGIN      
        1 attgtctaga ccaatatttc actaaaaata agatagtaaa tgattagtta atagttaggg
       61 ttgtgtattt aggttttacc tgcgaaatac acttgagcag gtgttttata atcaagagat
      121 tgatgtaatc gcttgtgatt ataaaactca aaataagcct ttaagccgat tctagcattt
      181 tgtactgttt ggaagtcctg caagtaaaca cactcatact tcacactgcg ccataatcgc
      241 tcgacaaaaa tattgtccaa tgcccgacct cggccgtcca tactgacctt aatatcacca
      301 tctaataaag gtttggtaaa cttaggcgtg gtgaattgcg cgccttggtc agtattaaat
      361 atctcgcacc gtctaccttg taacagcaat gactcaaccg cctctataca aaaatctgcc
      421 tctagcgttg tgcttaatga ccatgtcaac acataacgac tatgccagtc aatcactgcc
      481 atcagataca caaagccctt agcaagccga atgtaagtga tgtcagtact ccaaacatgg
      541 tcacaatgcg taatcgctac atgacgtaat aagtaaggat aaatcacatg ttcagggttt
      601 gcctgactgg tattgggctg aggatatatt gcctctaaac ccagcagccg caacagcctt
      661 ctcacgcgct tagggttaat acggtaacct ttgcctctta aatacgccgt catgcgctta
      721 accccataaa aaggcgtttc ggtgtattgc tcatccaata aacgcatcaa tacgaggttc
      781 tctgcacttt cttcacatgg acgataatac agactagaac ggtttaagcc taatagcgca
      841 cattgttgcc tgagactaaa caaaccatcg tctttatcca aaagagcttg ccgctgagct
      901 accccaactt cgcatacttt tttttaagcc agtctctttc agcaatcact tgacccagtt
      961 gtcgatgcag ttcgtctatg acggcttgtt ggtcttgtcc ctctgttgct tgcttcttgc
     1021 taaatgcgct cgtaatcg
//
LOCUS       DAOQMB010000883         1038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_140631_length_1038_cov_2.957274, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000883 DAOQMB010000000
VERSION     DAOQMB010000883.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_140631_length_1038_cov_2.957274"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLY05_14735"
     CDS             complement(<1..973)
                     /locus_tag="PLY05_14735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HNC04595.1"
                     /translation="MENKTLMTLLQNPNMNQTWEDFLEQAIIYYLCGGEAPIYGDAII
                     PTRLPKELIILRPDWLTPTLSNQFVGQIAEWRYTASDNASVSMSISPANLLMFKAFDP
                     LCRYRGLSPLSPAAFAIDQLNEYNKTNFALLQNGMQPSGSLTTEQVIDDAQFDRLKTQ
                     FSETYTGSQNNGKPLILEGGLKWQPFGFNLRDAEFLGGKTSSKKDICEALGVPTQLIG
                     IEGSQTYANYEQARAAFYEDRAIPLLDKFLAALNKWLGLRAGLRPDDIICVDIDAVAA
                     LEPRRAERNQVLDRIQSISINEKRTAMGYDAVDGGDGILINSGLIPLD"
BASE COUNT          284 a          245 c          201 g          308 t
ORIGIN      
        1 tgtctaacgg tatcaatcca ctatttatca aaataccatc gccaccatca accgcatcat
       61 agcccatagc agtgcgcttt tcgttaatgc tgattgattg tattctgtct agcacctgat
      121 tacgttctgc acgtcttggt tcaagtgcgg ccaccgcatc aatatctaca caaataatat
      181 catcgggtcg caacccagcg cgtaatccta accatttgtt taacgcagct aaaaacttat
      241 ctaaaagtgg gattgctcta tcttcgtaaa atgccgccct tgcctgctca tagttagcat
      301 aggtttggct gccttcaatg ccaattaact gagttggcac gcctaacgct tcgcaaatat
      361 cctttttgct tgatgttttg ccgcctaaaa actcagcatc gcgaagatta aagccaaatg
      421 gctgccactt taagccgcct tctaaaatca aaggcttgcc gttgttttgt gagcctgtgt
      481 aagtctcgct aaactgcgtc ttgagcctgt caaactgggc atcatcaata acttgttctg
      541 tggttaatga gccgctcggc tgcataccgt tttgcaacaa tgcaaaatta gttttgttat
      601 attcattaag ttggtcaatc gcaaaagcag caggggacaa tggcgacaat ccacgataac
      661 ggcataacgg gtcaaacgct ttgaacatca acaaattggc aggggatata ctcatgctta
      721 cacttgcatt gtcagacgct gtgtatcgcc attctgcaat ctgcccaaca aattgatttg
      781 ataatgttgg tgtgagccaa tcaggccgta aaataattaa ctctttcggt aatcgtgtcg
      841 ggataattgc atcgccataa attggtgctt caccaccaca caaataatag ataattgctt
      901 gctccaaaaa atcctcccat gtttgattca tgttcggatt ttgtagcaag gtcatcaaag
      961 ttttgttctc gataacagtc tcaccgcgct taatgatgat tgggcaagac gcgcacgctt
     1021 ttacatattc ttgaatac
//
LOCUS       DAOQMB010000884         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_141088_length_1036_cov_16.081549, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000884 DAOQMB010000000
VERSION     DAOQMB010000884.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141088_length_1036_cov_16.081549"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="PLY05_14740"
     CDS             <1..702
                     /locus_tag="PLY05_14740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNC04596.1"
                     /translation="LAVANRYQLWRIAKPTSWPRLLMYHSIAPEAPTGMNTPPEVFEA
                     QVAWLKKQGYHFCTVTELLANPATKKVAITFDDGFANNFHYAFPILQKYQAKATIYLA
                     PQIADIDKLTHQQIQTMQASGLIEFGAHSVSHINLAQTPANQAQQEIQQSKQQVEQIT
                     GQPCHSFAYPFGRFNEQVVAWVQQAGFRSAVTTQKKIIANPQQQPLTIPRISTHGAMD
                     KGQFYVAFSRGRYKF"
     gene            699..>1036
                     /locus_tag="PLY05_14745"
     CDS             699..>1036
                     /locus_tag="PLY05_14745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNC04597.1"
                     /translation="MIPVSVYIITKNCEPTLRQTLASVADFAEIILVDSGSTDNTIEI
                     AQEFNCKIYHQDWLGFAKQKQLALSYCKSEWVLNLDGDEVVSPLLKQEIIDCVNNNAV
                     DGLDIPIEDY"
BASE COUNT          315 a          206 c          216 g          299 t
ORIGIN      
        1 ttagcggtgg ctaatcgtta tcagttatgg cgtattgcta aaccaacatc atggccacgc
       61 ttattgatgt accatagtat tgcacccgaa gcaccaacgg gtatgaatac gccaccagag
      121 gtgtttgagg cgcaagtggc atggctaaaa aagcaaggtt atcatttttg tacggtcaca
      181 gaattattgg caaatccagc cactaaaaaa gtggcgatta cttttgatga cggttttgcc
      241 aataatttcc actatgcttt tccaattttg caaaaatacc aagccaaagc gaccatttat
      301 ttagcgccac aaattgctga tattgacaaa ttaacgcacc agcaaattca aaccatgcaa
      361 gcttctggcc tcattgaatt tggcgcgcat agtgtgagtc atatcaattt ggcacaaaca
      421 ccagccaatc aagcacagca agaaattcaa cagtctaaac agcaagttga gcagattact
      481 ggccaacctt gccatagttt tgcctatcct tttggtcgtt ttaatgagca agtagtggcg
      541 tgggtgcagc aagcaggttt tcgtagtgct gtcaccaccc aaaagaagat tattgccaat
      601 ccccaacagc agcctttaac aattcctcgt attagcacgc atggcgcaat ggataaaggc
      661 cagttttatg tagcgtttag tcgtgggaga tataaatttt gattccagtc agtgtgtaca
      721 ttatcaccaa aaattgcgaa cccacattaa ggcaaacctt ggccagtgtg gctgattttg
      781 ccgaaattat cttggtggat tcaggtagta ccgacaatac cattgaaatt gcccaagagt
      841 ttaactgtaa aatttatcat caagattggt tgggttttgc taaacagaaa caattggcct
      901 taagctattg taaatctgag tgggtattaa acttagatgg cgatgaagtt gtttcgcctt
      961 tgttaaaaca agaaattatt gactgtgtga ataataacgc tgttgatggt ttagatattc
     1021 ccattgaaga ctattt
//
LOCUS       DAOQMB010000885         1036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_141096_length_1036_cov_5.997961, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000885 DAOQMB010000000
VERSION     DAOQMB010000885.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141096_length_1036_cov_5.997961"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLY05_14750"
     CDS             complement(<1..916)
                     /locus_tag="PLY05_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04598.1"
                     /translation="MSGFVARLATAADNAAILALMSQPQPSNGVQFAFERLPNYFDSA
                     SVTHQHPHTIVIEKQNDKRLVAMVNMGWREVFVDGHLQRIRYGADLRIDKQFQGGRVL
                     MYINRAVRDCVQEDWYQSVILSANDKSRSALEGGRAGLPIYRSLGTISTHTITGRKAL
                     FSPHKQIRRAQVADIPAMNAFVKQMAEHYQFLPRYDFYQLAHQHPYFNGLSIDDFLVL
                     FDDEQLVGLVGLWNQKGFKQTRVVDYNRTVAMFRPIYNWWSRLTGGFILPAKGQVVDY
                     IALHSPLTHPKDGDSFRHLLHHAWLEVRQ"
BASE COUNT          331 a          239 c          192 g          274 t
ORIGIN      
        1 gttgacgaac ttccaaccac gcatgatgta ataaatgacg aaaactatca ccatctttag
       61 gatgagtaag tggcgaatgc aatgcaatat aatcaacaac ttgcccttta gcaggcaaaa
      121 taaaaccgcc cgttaaacgg ctccaccaat tataaatagg tctaaacata gcaaccgtac
      181 ggttgtaatc aacaactcgt gtttgtttaa agcccttttg gttccacaaa cctaccaagc
      241 cgactaattg ctcatcatca aataacacta aaaagtcatc aatgcttaaa ccattaaagt
      301 aaggatgctg atgagctaat tgataaaaat cataacgagg taaaaattga tagtgctcag
      361 ccatttgttt tacaaaggca ttcattgctg gaatatctgc aacctgtgca cgtcgtattt
      421 gtttgtgtgg ggaaaataag gctttacggc ctgtaatagt atgggtgcta attgttccta
      481 atgagcgata aatcggcagc cctgctctgc caccttctaa tgctgaacga gatttatcat
      541 tcgcacttaa aatgaccgat tgataccaat cttcttgcac acaatcacga actgcgcgat
      601 tgatatacat taaaacacga ccaccttgaa attgtttgtc aatgcgtaag tcagcaccat
      661 aacgaatacg ctgcaaatga ccatcaacaa acacttcacg ccatcccata ttcaccatgg
      721 caactaaacg cttatcgttt tgtttttcaa taacaatcgt atgtgggtgt tgatgggtaa
      781 cactggccga atcaaaataa tttggtaaac gctcaaacgc aaattgcaca ccattagacg
      841 gttgaggttg ggacatcaac gccaaaattg ccgcattatc agcggctgtt gccaatcgtg
      901 cgacaaagcc actcatgata ccttgtccca ttgctgatct aaaacaagct gagccgaagg
      961 taataaacgc tcgatcgccg cacgttgcac acggcgaact ggctctggtt taatgttacc
     1021 ttcaatcatg ccacct
//
LOCUS       DAOQMB010000886         1035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_141364_length_1035_cov_5.563265, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000886 DAOQMB010000000
VERSION     DAOQMB010000886.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141364_length_1035_cov_5.563265"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /gene="recA"
                     /locus_tag="PLY05_14755"
     CDS             complement(<1..793)
                     /gene="recA"
                     /locus_tag="PLY05_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HNC04599.1"
                     /translation="MDDNKRKALGAALAQIEKQFGKNTLMRLGDKPTEAIQAVSTGSL
                     GLDIALGIGGLPKGRIVEIYGPESSGKTTLTLQVIAECQRQGGTAAFIDAEHALDPTY
                     ARKLGVDTDNLYVTQPDNGEQGLEIADMLVRSGAVDVIVIDSVAALTPKAEIEGDMGD
                     SHMGLQARLMSQALRKITGNAKRTNCLVIFINQLRMKIGVMFGSPETTTGGNALKFYA
                     SVRLDIRRTGAVKDGEEVVGSETKVKVVKNKVAPPFRETTFQIMYG"
BASE COUNT          292 a          233 c          205 g          305 t
ORIGIN      
        1 gaccatacat aatttggaaa gtggtttcgc ggaatggtgg agcaaccttg tttttcacca
       61 ctttaacttt agtttcagag ccgacaactt cttcaccatc tttgaccgca cctgtacgac
      121 gaatatctaa acgtacggaa gcataaaact taagggcatt accgcctgtt gtggtttcag
      181 ggctaccaaa catcacacca attttcatac gcaattggtt aataaaaatc actaaacagt
      241 tagtacgttt ggcattaccg gtaattttac gcaaggcttg actcatcaac cgtgcttgta
      301 aacccatgtg cgaatcaccc atgtctcctt caatttcggc tttaggagtg agggcggcca
      361 ccgagtcaat cacaattaca tcaactgccc cagaacgtac taacatatcg gcaatttcaa
      421 gaccttgttc gccattatct ggttgagtga catataagtt gtcggtatca acacctagtt
      481 tacgcgcata agtcgggtca agtgcatgtt cggcatcaat aaacgcggct gtaccacctt
      541 gacgttggca ttcagcaatc acttgtaagg tgagtgtcgt tttacccgaa gattcaggac
      601 cataaatttc aacaatacga cctttaggta aacccccaat accaagggct atatctaacc
      661 ctaaagagcc tgttgaaacc gcttgaatgg cttcggttgg tttgtcacct aaacgcatta
      721 aggtgttttt accaaattgt ttttcgattt gcgctaaagc agcaccaaga gctttacgtt
      781 tgttatcatc cattttttat ctcctaaaga gtgatgtagg ttgtggattg gatggctgta
      841 tagtcgcagg ttaattggct tttggctaga ggtcaaaaaa tgtcgtgcgt cataaatcgt
      901 cgctatttaa tactattatc catgactaat cctcgttaaa cttgatactc aatttttagg
      961 actaaacaga gcaacgactg tgactgattt tgccgaccac accccgatga tgcagcaata
     1021 tttacgcatc aaagc
//
LOCUS       DAOQMB010000887         1033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_141914_length_1033_cov_13.267894, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000887 DAOQMB010000000
VERSION     DAOQMB010000887.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_141914_length_1033_cov_13.267894"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="PLY05_14760"
     CDS             <1..>1033
                     /locus_tag="PLY05_14760"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HNC04600.1"
                     /translation="TKDFNGTVQLSYQVSDGNNGLTSASNSFLVNKANNTAPSSVTPI
                     SDQYTEESKGFNFQLVQDAFVDLDGDILTYSAILEDGSDLPSWLSFDAVTRTFSGTPS
                     FDDASVLRVKVVASDGQATASQIFTLDVLDVNRAPIHISNTPFVMDEDGGSVKIDVLA
                     RVQDPDGDSYVISNVNVDAAQGSIVLNADKTISFTPTKDFNGTAQVSYQVDDGRGGVV
                     QITENIVVNPVNDAPVLSSTAAVLPNGTEDQSYLIDVNQLLQGFSDVDGGTLSVVNLQ
                     ATNGSLVDNQNGTYSFNPTKDFNGTIQLTYQVSDGNNGLTSASNSFVINAVNDAPVLS
                     STAAVLPNGT"
BASE COUNT          315 a          195 c          242 g          281 t
ORIGIN      
        1 ccacgaaaga cttcaacggc acggttcagt taagctatca agtgagtgat ggcaacaatg
       61 gcttaacctc agcgagcaat agctttttag tgaataaagc caataatacg gcacctagta
      121 gtgttactcc gattagcgat caatacacgg aagaaagtaa aggttttaac tttcaattag
      181 tacaggatgc ctttgttgat ctagatggcg atattttaac atatagcgct atattagaag
      241 acggcagtga tttacccagt tggttaagct ttgatgcagt gacacgcacc tttagtggga
      301 caccaagctt tgatgatgct agtgttctac gtgtgaaagt agttgctagt gatggtcaag
      361 cgacagcaag tcaaatattc acgttagacg ttttagatgt aaatcgcgca ccgattcata
      421 taagcaacac tccattcgta atggatgaag atggtggtag tgtaaaaata gatgtattgg
      481 cacgagtcca agaccctgat ggtgattcat atgtcatcag taatgtcaat gttgatgcgg
      541 cacaagggag tatagtgtta aatgcggata aaacgattag tttcaccccc accaaagatt
      601 ttaatggtac ggcgcaggtc agttatcaag tggatgatgg acgaggtggt gttgttcaga
      661 ttacagaaaa catcgttgtt aatccagtca atgacgcacc agtcttaagt tctaccgctg
      721 cggttttacc aaatggcaca gaagaccagt catacctcat tgatgtcaat cagctattac
      781 aaggctttag tgatgtggat ggcggcactt taagtgtggt gaatctacaa gcgacaaatg
      841 gcagccttgt ggataatcaa aatggcacat atagctttaa cccaacgaaa gacttcaacg
      901 gcacgattca attaacctat caagtgagtg atggcaacaa tggtttgacc tcagcgagta
      961 atagctttgt gatcaatgcg gtgaatgacg caccagtctt aagttctacc gctgcggttt
     1021 taccaaatgg cac
//
LOCUS       DAOQMB010000888         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142210_length_1032_cov_15.570113, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000888 DAOQMB010000000
VERSION     DAOQMB010000888.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142210_length_1032_cov_15.570113"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /gene="thrC"
                     /locus_tag="PLY05_14765"
     CDS             complement(<1..608)
                     /gene="thrC"
                     /locus_tag="PLY05_14765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNC04601.1"
                     /translation="MRYTGLINRYRDYLPISDDTRIISLGEGNTPLIRLKNIPKLIGK
                     DVDIYVKYEGLNPTGSFKDRGMTMAVTKAVEEGSKAIICASTGNTSAAAAAYAARAGI
                     KAFVLIPEGKIAMGKLAQAMMYGAITLQIRGNFDDGMELVKQVASQAPVTIVNSVNPY
                     RLQGQKTAAFEIVEELGRAPDYHCLPVGNAGNITAHWIGYCE"
     gene            complement(667..>1032)
                     /locus_tag="PLY05_14770"
     CDS             complement(667..>1032)
                     /locus_tag="PLY05_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007395698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNC04602.1"
                     /translation="DIVRSLSIDNASGVPHLAFQPDSLADTPILPVEAIKTAYYLRLQ
                     ANDKSGVLADVTRILSDNGISIEAILQKPAHEAGTVPVIILTKPVIEREMNRAIGGLE
                     ALADIVAPVVRVRLEALDA"
BASE COUNT          308 a          244 c          200 g          280 t
ORIGIN      
        1 tattcgcaat aaccaatcca atgcgcggta atattacctg cattaccaac tggcaaacaa
       61 tgataatcag gcgcacgacc taactcttcc acaatttcaa aggcagcggt tttttgacct
      121 tgtaaacgat acggattcac tgagttaaca atcgtgacgg gcgcttggct ggccacttgt
      181 ttaaccaact ccatgccatc atcaaaatta ccacgaattt gtaaggtaat cgcaccatac
      241 atcattgctt gggctaattt acccatggca attttgcctt ctggaatcaa tacaaaggct
      301 ttaatgcctg cacgcgcggc ataagcagcg gcagccgcag aggtattacc tgtagaggca
      361 caaataatgg ctttgctacc ctcttcaacc gcttttgtca ctgccatggt cataccacgg
      421 tctttaaaag aacctgttgg atttaagcct tcatatttga cataaatatc gacatctttg
      481 ccaatcagct tgggaatatt ttttaagcga attaaaggtg tattaccttc acctaaagaa
      541 ataatacggg tatcgtcaga aatcggtaaa taatcacggt atctgttaat taaacctgtg
      601 tagcgcataa ttttctctct acaaaaacat ctactctcac tcattgagcg agaggctggg
      661 aatgggttaa gcgtctaagg cttctaaacg aacacgcacc acaggggcaa caatatccgc
      721 taaggcttct aaaccaccaa tcgcacgatt catttcgcgc tcaatgactg gcttagttaa
      781 aataatcaca ggcactgtgc ctgcttcgtg ggcaggtttt tgtaaaatcg cttcaatgct
      841 aatgccatta tcactcaaaa ttcgcgtcac atcggccagt acacccgatt tatcattagc
      901 ttgcaaacgt aaatagtaag cggttttaat ggcttctaca ggcaaaatgg gcgtatccgc
      961 caatgagtca ggttgaaagg ctaaatgtgg cacaccactg gcgttatcaa tactcaaaga
     1021 gcgcacaata tc
//
LOCUS       DAOQMB010000889         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142212_length_1032_cov_13.786080, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000889 DAOQMB010000000
VERSION     DAOQMB010000889.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142212_length_1032_cov_13.786080"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..903
                     /gene="rfbD"
                     /locus_tag="PLY05_14775"
     CDS             16..903
                     /gene="rfbD"
                     /locus_tag="PLY05_14775"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009584324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HNC04603.1"
                     /translation="MARILLTGVSGQVGHELLKMLTPLAEVIAVTRQDMDLTNQASIL
                     QILEKYQPTIIVNPAAYTAVDKAESEQEQAYLINRDAPKTMAEWAAANNALLVHYSTD
                     YVFDGTKEGSYVEEDIPNPQSVYGLSKLLGEQAIVQSGAKHLIFRTSWVFGVHGNNFI
                     KTIIRLAKERESLNVVADQHGAPTSARLIAQVTTQVLAQYLQDAQFNAYGVYHLTSNG
                     QTTWHEYAQFIVEQTKLKQDIILLKDNIKAIPSSAYPVPAKRPTNSCLNCDKIKKAFD
                     VDLTHWQIDVLSVIDAVCQ"
     gene            920..>1032
                     /locus_tag="PLY05_14780"
     CDS             920..>1032
                     /locus_tag="PLY05_14780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005214418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HNC04604.1"
                     /translation="MQRKGIILAGGSGTRLYPATLAVSKQLLPIYDKPMIY"
BASE COUNT          352 a          160 c          210 g          310 t
ORIGIN      
        1 tatggagtat aaaaaatggc gagaatttta ttaacaggtg tcagtggtca agtagggcat
       61 gagttattaa aaatgttaac tccattggct gaggtgattg cagtgactcg tcaagacatg
      121 gatttaacca atcaggcgag tattctacaa attttagaaa aatatcaacc caccattatt
      181 gtcaatcctg ctgcttatac cgctgttgat aaagctgagt cagagcaaga acaagcgtat
      241 ttgattaatc gtgatgcacc aaaaacaatg gcagaatggg cagcagcgaa taatgcctta
      301 ttggtacatt attcaactga ttatgtcttt gatggaacga aggagggtag ctatgttgag
      361 gaagatatac caaatccgca atctgtgtat ggtctgagta aattattagg cgaacaagcg
      421 attgtgcaaa gtggggcaaa acatcttatt tttagaacca gttgggtgtt tggtgtacat
      481 ggtaataact ttattaaaac tattattcgt ttggccaaag aacgtgaaag cttaaatgtg
      541 gttgccgatc aacatggtgc accaacatca gcacgtttaa ttgcacaagt gacaacacaa
      601 gtattagcgc aatatttgca agatgcacag tttaatgctt atggtgttta tcatctaaca
      661 agcaatggtc aaacaacatg gcatgaatat gcacaattta ttgtcgaaca aactaaatta
      721 aagcaagata ttattttgtt aaaagataat ataaaagcta ttccaagtag tgcatatcct
      781 gttcctgcca aacgtcccac aaattcttgt ttaaattgtg ataaaataaa aaaagcattt
      841 gatgttgatt taacacattg gcaaatagat gtgttaagtg tgattgatgc ggtttgccaa
      901 taattgagga agttaaatca tgcaacgtaa aggtattatt ttagcaggtg gctctggtac
      961 acgtttatat cctgcaacgt tagcagttag caaacaactt ttgccaattt atgacaaacc
     1021 aatgatttat ta
//
LOCUS       DAOQMB010000890         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142227_length_1032_cov_5.558854, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000890 DAOQMB010000000
VERSION     DAOQMB010000890.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142227_length_1032_cov_5.558854"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1032
                     /locus_tag="PLY05_14785"
     CDS             <1..>1032
                     /locus_tag="PLY05_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNC04605.1"
                     /translation="NTPGPVHLSIPLDVMRTKIEDRQVSSMRAFINHEVIPSQEAMQL
                     VLKELAMTAKVTVVIGEGAAGAINAILALIETRDWLFVTTPRAKGLVNSYHPLYRGVF
                     GFAGHESAKEALLPSNAERVVVIGTALDEVSTSGWDSSTIMSNRLIHLSNNPEHLSRS
                     YMAKLCILGSLELMLKPFMDFLRTNSARRKWFMQQDAYGLPINLSIQQPQSCLDHNGK
                     VKPQALMTYMSDVCPDETRALFDSGNSFLWGIHYWNCRRPKGFDAHKSLFHIGIGFAS
                     MGWAIGAAVGVAAGAKGHPVVCVTGDGSMLMSGQEITVAAQNQFGVMFVVLNDSSLGM
                     VRHGQKLSGG"
BASE COUNT          275 a          178 c          259 g          320 t
ORIGIN      
        1 taacacgcct ggccctgtgc atttaagtat tccgttggat gtgatgcgta cgaaaattga
       61 agatagacaa gtgtcttcaa tgcgagcatt tattaaccat gaggtgattc ctagccaaga
      121 agcaatgcag ttagtactaa aagagttggc gatgactgct aaagtaacag tggtgattgg
      181 cgagggcgca gcaggagcta ttaacgcgat tttagcactc atcgaaactc gtgattggtt
      241 atttgttacc acccctcgcg ccaaaggctt ggtcaatagt tatcatccat tatatcgtgg
      301 tgtatttggt tttgcaggtc acgaaagtgc taaagaagct ttattaccaa gcaacgcgga
      361 gcgagtggtc gttattggta ctgctttaga tgaagtctca accagtggtt gggatagcag
      421 cactattatg tctaatcgtt tgattcattt atccaataat ccagagcatt taagtcgttc
      481 atatatggct aagctatgta ttttaggctc attggagtta atgctaaagc cttttatgga
      541 ttttttgcgt actaatagtg ctcgacgtaa atggtttatg cagcaagatg cttatggttt
      601 gcctatcaac ctcagtattc aacaaccaca aagctgtttg gaccacaatg gcaaagttaa
      661 accacaagca ttgatgactt atatgagtga tgtttgtcca gacgaaaccc gtgcattatt
      721 tgacagtggt aatagttttt tgtggggcat tcactattgg aattgtcggc gacctaaagg
      781 ttttgatgct cataaaagtc tatttcatat aggaattggt tttgcctcaa tgggttgggc
      841 aattggtgcg gctgttggtg tggcagcagg tgcaaaagga caccctgtgg tttgtgtgac
      901 aggcgatggc agtatgttaa tgagtggcca agaaattacc gttgctgcac aaaatcaatt
      961 tggcgtgatg tttgtggtgt taaatgattc ctctttaggc atggtgcgtc atggtcaaaa
     1021 gctgagtggt gg
//
LOCUS       DAOQMB010000891         1032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142294_length_1032_cov_3.129990, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000891 DAOQMB010000000
VERSION     DAOQMB010000891.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142294_length_1032_cov_3.129990"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="PLY05_14790"
     CDS             <1..450
                     /locus_tag="PLY05_14790"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNC04606.1"
                     /translation="CDFIAPIKRYTGTLYSPEAQKSLQTQHQLALANCLAQSRVLMLG
                     DASTDSDENTPNFKRYRGNQPSTTLLLDELTPYTLGALVALYEHKVFVMSVIWDINPF
                     DQWGVELGKKIADTMTKALSNDHQHGFDASTNYLLATINGAVQGANV"
     gene            447..>1032
                     /locus_tag="PLY05_14795"
     CDS             447..>1032
                     /locus_tag="PLY05_14795"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04607.1"
                     /translation="MKVCVHGDTLFAWIAAAKLAETGNQVVIRTSEVNYSFDPSREPN
                     LLSLLDEQQLAKRLVTASLVDDIPAHVGIHLIALDLPVDEIEVLVKQVICQVDSQQPL
                     LFLILSTLPIGSFDRLQTYANNVLVSERPQAQADVIGLPLFCREGTALLDFSEPENLL
                     ISGENGSYAITQTLELLRPFVRKATHIMVVPHTTA"
BASE COUNT          308 a          187 c          211 g          326 t
ORIGIN      
        1 tgtgatttta ttgcaccgat taaacgttat acaggtactt tatattcacc agaagcacaa
       61 aaatctttgc aaacacaaca ccaattagct ttagcaaatt gtttggcaca atcgcgcgtt
      121 ttgatgttgg gtgatgccag tacagatagc gatgaaaata ccccaaactt taaacgttat
      181 cgtggcaatc aaccgtctac aaccttgtta ttagatgaat taactcccta cactttaggt
      241 gcattagtag ccttgtatga gcataaagtt tttgtcatgt ctgtgatttg ggatattaat
      301 ccctttgatc aatggggagt agagttaggc aaaaagattg ccgatacaat gaccaaagca
      361 ttatcaaatg atcaccaaca tggttttgat gcctcaacca attatttgtt agcgacaatt
      421 aacggcgcag tacaaggggc aaatgtatga aagtctgtgt acatggagat accttattcg
      481 catggattgc cgccgcgaaa ttagcagaaa caggcaatca agtggtaatt cgtaccagtg
      541 aggttaatta tagttttgac ccttcaagag aacctaatct attatcatta ctggatgagc
      601 agcaattagc aaaacgatta gtcacggctt cattggttga tgatattcct gcacatgtgg
      661 gtattcattt aatagctttg gatttacccg ttgatgagat tgaagtttta gtaaaacaag
      721 ttatttgtca ggtagattcg cagcaaccat tgttgttctt aattttatcg acgttaccga
      781 ttggtagttt tgaccgctta caaacctacg caaataacgt tttagtgagt gagcgccctc
      841 aagctcaagc tgatgttatt ggtttacctt tattctgtcg tgaaggtacg gcattattag
      901 attttagtga gccagaaaac ttattgatta gtggtgaaaa tggtagttat gcgattacac
      961 aaactctaga gttattgcga ccttttgttc gtaaagcaac ccatatcatg gttgtgccac
     1021 ataccactgc tg
//
LOCUS       DAOQMB010000892         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142515_length_1031_cov_13.238730, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000892 DAOQMB010000000
VERSION     DAOQMB010000892.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142515_length_1031_cov_13.238730"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(529..>1031)
                     /locus_tag="PLY05_14800"
     CDS             complement(529..>1031)
                     /locus_tag="PLY05_14800"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNC04608.1"
                     /translation="MSREVIDAFFGDEDAYDRYTMARIEVMQDKSLSATEKAKRTAEL
                     LNTLPPHLKESVQTLNKYQELTTLTDDWKARGGKPEELRAIREQIVGAEATERLEALD
                     QERAEWDKRINAYLQTRDTILKNPALSDAERQRQLNEIKNKDFTEQERVRVGAFETMH
                     DEGVKP"
BASE COUNT          294 a          202 c          166 g          369 t
ORIGIN      
        1 aatcaataaa acccactaac ttttcaaaac ctaatacccc tggtgctaac acaatcggat
       61 acttagtttt ggtataagta cagtcttccc accaccaaca agcttgtgct tgagtagcaa
      121 ccatagaact ggctaataaa gttgcagtcg caagtaattt tttcatggtt attgtcccct
      181 gttgttgtct ttgtcgtgtg tcgatttttg tttattaaat tattaatatt atttttacag
      241 ggtatatgaa tttatgaaat ttagagtatt tactttaatt attggatgca attttagcct
      301 aaaaaaaaaa ctactccagt gacattttag attatagatg tcattttttt gcttttttgc
      361 gacaaagtga taaaagatag caactaaatg acaaacggcc actaatagtg gccgtttata
      421 tagagaactt acgcatttca ccgaaattag catgttttta gagtataaaa tcgttagcaa
      481 tggttaaagt acaaaccgca taagtcccag tttagctatt aataactttt agggtttaac
      541 accttcatcg tgcatggttt caaaggctcc tactcgcact ctttcctgtt ctgtaaaatc
      601 tttatttttg atttcattta actgacgttg acgttctgca tcacttagtg cggggttttt
      661 caagattgta tcgcgggttt gtaaataagc attaatacgt ttatcccatt ctgctctctc
      721 ttgatctaat gcttctaaac gctcagttgc ttcagcaccc acaatttgct cacgaatagc
      781 acgcaattct tctggtttgc cacctcttgc tttccagtca tccgttaacg ttgttaactc
      841 ttgatattta ttcagcgttt gaacactttc ttttaaatgt ggaggtaagg tgtttaataa
      901 ctctgctgta cgtttagctt tttcggtagc acttaaactt ttatcttgca tcacctcaat
      961 acgcgccata gtgtaacgat cataggcatc ttcatcacca aaaaaagcat caatcacttc
     1021 gcgagacata t
//
LOCUS       DAOQMB010000893         1031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142526_length_1031_cov_6.197746, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000893 DAOQMB010000000
VERSION     DAOQMB010000893.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142526_length_1031_cov_6.197746"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLY05_14805"
     CDS             <1..191
                     /locus_tag="PLY05_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04609.1"
                     /translation="VYRDGSLIATTPHPNWVSASQFIQLGKDGDSQYSNGGMDEFLLY
                     DDVLWTADFTPPTTPFSC"
     gene            386..>1031
                     /locus_tag="PLY05_14810"
     CDS             386..>1031
                     /locus_tag="PLY05_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04610.1"
                     /translation="MAKYTKPNYAEQWASAGNIVAPNSAKIQAGHIVEKPNYEYMNWL
                     QNRQDTAIAYSLQMGIPEWDNTTQYQYTSNSHASYVQRNGVVYKAIQTGTNKDPLTEA
                     AYWTIAFDDKGMAATVQSNLNAHITNYGTLTGLTDTATARTNLDVYSKTEAAQKNGST
                     SETFSVATATSADHAVRKDQLDAKANKNGSSSETFSVDTATAAAHAVRKDQFDAK"
BASE COUNT          348 a          200 c          204 g          279 t
ORIGIN      
        1 aagtctatcg ggacggtagc ttaatagcta caactccgca tcctaactgg gtgtcggcct
       61 cacaatttat tcagctaggc aaagatggag attcacagta ttcaaatggt ggaatggatg
      121 aatttttgtt atatgacgat gtgttgtgga ctgctgattt tacgccacca acaactcctt
      181 ttagttgtta agtaacctcc ccaaaaccca tctacaaaca gcgtaaacta tgcttatctc
      241 tttattaaga taggcgtgtt tattatgcca aaaatggtaa aatgagtgtt gaggttcaac
      301 agtggtatta ccaacgatgg agattcgtca gacatttgta catactctat gtacaattta
      361 aaatcacaat aatactaggt gaaacatggc aaaatataca aaaccaaact acgcagaaca
      421 atgggctagt gctggcaaca ttgtagcacc aaacagtgct aaaatacaag cagggcatat
      481 cgttgaaaaa ccaaattatg agtacatgaa ttggcttcaa aacagacaag atactgctat
      541 tgcttattcg ttacagatgg gtattcctga gtgggataac acaacacaat atcaatacac
      601 ctctaacagt catgcttctt atgtacaacg taacggtgtt gtgtataagg cgattcaaac
      661 agggacaaac aaagaccctt tgacagaggc agcttattgg actattgcgt ttgatgataa
      721 aggtatggct gctacagtac aatctaacct taacgctcat atcaccaatt acggcacttt
      781 aacaggtctt acagatactg caacagctag aacaaatttg gatgtttatt ctaaaactga
      841 ggctgctcaa aaaaacggta gtacctctga aacatttagt gttgcaacag ccacgtctgc
      901 ggatcatgct gttcgtaaag accaattaga tgctaaagcc aataagaacg gtagtagtag
      961 tgaaactttt agtgtagata ctgcaacagc agcagcacac gctgttcgta aagaccaatt
     1021 tgatgctaag a
//
LOCUS       DAOQMB010000894         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_142825_length_1030_cov_7.145641, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000894 DAOQMB010000000
VERSION     DAOQMB010000894.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_142825_length_1030_cov_7.145641"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..>1030)
                     /locus_tag="PLY05_14815"
     CDS             complement(220..>1030)
                     /locus_tag="PLY05_14815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04611.1"
                     /translation="LVKTADCSDEVTAVVAQALQDKSQQLGASGWHVLFVDEQELAQW
                     VTTGADMRLGCQFHWYNEGYTDFDSFLVAMNSKKRRTLRKERNSLIAQGIEFKRLTGD
                     AITPMLWQRFQAFYQLTNLKYNRHHGYLTPDFFERIYHTMRDSLLLVVAFEGDEAIAG
                     ALYFLGKDTLYGRYWGCIREVEFLHFETCYYQGIEYCIEQGIQRFDSGAQGEHKVPRG
                     FRPTLTYSVHWLKHAGFKQAVKRFLVEESLAIQRYRDEMNQALPFRQVEGD"
BASE COUNT          309 a          236 c          193 g          292 t
ORIGIN      
        1 tcatcgtagc cgttgtagca tttgtgatat gcctccgtgt cgttgtttat gttgacactg
       61 tgcatgacca ctagaagtga tgctcagtgc aaggtatagc cgagatgtaa ggttgcgtga
      121 tggtactcaa ctttacactt ctttatgagc taataatgat atggcatatt tagctagagg
      181 ataattaaat gttatcttaa tgacgctaag ccaatttaac taatctccct caacctgcct
      241 aaacggtaat gcctgattca tctcatcacg ataacgctga atcgctaacg attcttcgac
      301 taaaaaacgt ttaacagctt gtttaaagcc agcgtgcttc agccaatgga cggagtaggt
      361 gagcgtcggt ctaaaaccac gcggcacttt atgctcacct tgtgcgccag agtcaaatcg
      421 ttgaataccc tgttcgatac aatattcaat gccttgataa taacaggtct caaaatgcag
      481 aaactctacc tcacgaatac agccccaata acggccatat aaagtatctt tgcctaaaaa
      541 atataatgcc ccagcaatcg cttcatcgcc ctcaaaagcc acaactaaca acaaactatc
      601 gcgcatagtg tgataaatac gctcaaaaaa gtcaggggtt aaataaccat gatgtcgatt
      661 atattttaga ttggttaatt gataaaaagc ctgaaaacgt tgccaaagca ttggggtaat
      721 ggcatcacct gtcagtcgtt taaactcaat tccctgagcg attaaactgt tacgctcctt
      781 acgcagcgta cggcgttttt tagagttcat tgccactaaa aagctatcaa agtcagtgta
      841 tccttcgtta taccaatgaa actgacagcc taaacgcata tcagctcctg ttgttaccca
      901 ctgtgctaat tcttgctcat caacaaacaa tacatgccaa cctgatgcgc ctaattgttg
      961 ggatttgtct tgtaatgcct gtgcgacaac agcagtgact tcatccgaac aatcggctgt
     1021 tttcaccagt
//
LOCUS       DAOQMB010000895         1030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143015_length_1030_cov_1.978462, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000895 DAOQMB010000000
VERSION     DAOQMB010000895.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143015_length_1030_cov_1.978462"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLY05_14820"
     CDS             <1..583
                     /locus_tag="PLY05_14820"
                     /inference="COORDINATES: protein motif:HMM:NF036754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNC04612.1"
                     /translation="NRQFICVQLDEKTDPKKEANKAGYQSIFDITQARIQKAAQKIQT
                     DNPNYTGDLGFKLFKTVPIFDNYQQPIAQFAPNQELFDGTQLSPQDLANLLTTWQLQH
                     NIPLSQTLNTIDLGGYTAHWFNDTLYLMAQGFDSPHLNAFIQKLDTDNSFTITKLIMF
                     GYNINSKMQREIDEGLKNYRNKKSITLDVVVKY"
     gene            583..>1030
                     /locus_tag="PLY05_14825"
     CDS             583..>1030
                     /locus_tag="PLY05_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC04613.1"
                     /translation="MKTFNTATDTPKRQFHFEGNLPHQVKAVASIANVFESIDFMPVT
                     GLLKDVANPQYANNPNIGNENSRFYYHALEKTRQSNQIFDDQSLKTDCLVQSLNNTCR
                     VLDIAMETGTGKTYTYTKAMFALNQQKGIFKFIVIVPTLAIKAGTIN"
BASE COUNT          363 a          264 c          155 g          248 t
ORIGIN      
        1 caatcggcaa tttatttgcg tacaactaga cgaaaaaacc gaccctaaaa aagaagccaa
       61 caaagcaggt taccaaagta tttttgacat tacccaagcc cgaatccaaa aagccgcaca
      121 aaaaatccaa acagacaacc ccaattacac aggcgactta ggctttaaac tgtttaaaac
      181 cgtgcctatt tttgataact atcaacagcc tattgcacag tttgccccca atcaagagct
      241 atttgacggc acacaattaa gcccacaaga ccttgccaac ctcctcacca catggcaact
      301 gcaacacaac atccccctta gccaaacact caacaccatc gacttaggcg gttatacggc
      361 tcattggttt aacgacacct tatatttaat ggcacaaggt tttgactccc cacacctcaa
      421 cgcctttatt caaaaactcg acaccgataa cagttttacc attaccaaac tgattatgtt
      481 tggctacaac atcaacagca aaatgcagcg cgaaattgac gaaggcttaa aaaactatcg
      541 caacaaaaag tctattactc ttgatgtggt ggttaaatac taatgaaaac ttttaacacc
      601 gccaccgata cccccaaacg tcaatttcat tttgaaggca atttgccgca tcaagttaaa
      661 gcggtggcca gtattgccaa tgtctttgaa agcatagact ttatgcctgt cacaggtttg
      721 ctaaaagacg tagccaatcc gcaatatgcc aataacccaa acataggcaa tgaaaacagt
      781 agattttatt atcacgctct cgaaaaaaca cgccaaagca accaaatttt tgacgaccaa
      841 agcctaaaaa ctgattgttt agttcaatca cttaataaca cttgtcgagt gttagatatt
      901 gccatggaaa caggcacagg caaaacctac acctacacca aagccatgtt tgccctcaac
      961 caacaaaaag gcatttttaa gtttattgtc attgttccta ccctagccat taaagcaggg
     1021 acaattaact
//
LOCUS       DAOQMB010000896         1029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143087_length_1029_cov_7.232033, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000896 DAOQMB010000000
VERSION     DAOQMB010000896.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143087_length_1029_cov_7.232033"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            336..>1029
                     /locus_tag="PLY05_14830"
     CDS             336..>1029
                     /locus_tag="PLY05_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HNC04614.1"
                     /translation="MNTNLQFADDDVVTSLRVPPHSLTLEQAVLAGLMMEPEVWDNLE
                     SVLGEQDFYSPRHRMIFGAIAHLIRLEQPCDALLVAKHMEDTGFLEKAGGEAYLEQIL
                     RDSPATTANILIYAERIREFSVLRQLITSTNEIANLAYHPKGESASTILDQAETKIFA
                     IAEQQKNRSTKNGPQLIKPLLAQTIQKIDELYHSTEPLTGLSTGFTDLDKRTSGLQGG
                     SLVIVAARPSMGK"
BASE COUNT          313 a          204 c          196 g          316 t
ORIGIN      
        1 attgcatagc gatgtaatgg cagaagttgc tgttactatc gttgctgagt aatctggtaa
       61 gtcttactag accactcaaa aaaagcacta ttgttttcaa caatggtgct tttttatttt
      121 gtcgtttatt tgctcacact gtaatggttt taacgatttc atgtgcgcaa gacgcgctaa
      181 tttcgatgaa atgtataagt cgtaatctct cgtttatagt ttgtcaagac taacaaaacg
      241 actaaaaaaa gcctattatc tacataggca cactgctctg taaaaattca aaaagtacac
      301 gacaaaccca acctcaattt gatatctgat tagctatgaa taccaatctg caatttgctg
      361 atgacgatgt cgtcacttcg ctacgagttc cacctcatag cttaacgctt gagcaagcgg
      421 tattggctgg cctgatgatg gagccagagg tttgggataa tcttgaatcg gtactgggtg
      481 aacaagattt ttactctccc cgccatcgga tgatttttgg tgcaattgcc catcttattc
      541 gtttggaaca accatgtgat gccttattgg ttgccaaaca catggaagat actggctttt
      601 tagaaaaagc tggtggtgaa gcttatttag aacaaatttt acgagactca ccagcaacca
      661 ccgccaatat tttaatttat gctgaacgta ttcgtgaatt ttcggtatta cgtcaattaa
      721 ttacctcgac caatgaaatt gctaatttgg cctatcatcc caaaggtgaa tcggcatcaa
      781 ccattttaga tcaagccgaa actaaaattt ttgcgattgc tgaacaacaa aaaaaccgta
      841 gcacaaaaaa tggcccacaa ctgattaaac ctttattagc gcaaaccatt caaaagattg
      901 atgagcttta ccattctacc gaaccattaa cgggtttaag tacaggtttt accgatttag
      961 acaaacgtac ctctggttta caaggtggta gtttggtgat tgttgctgct cgtccaagta
     1021 tgggtaaaa
//
LOCUS       DAOQMB010000897         1028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143369_length_1028_cov_6.261048, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000897 DAOQMB010000000
VERSION     DAOQMB010000897.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143369_length_1028_cov_6.261048"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="PLY05_14835"
     CDS             <1..712
                     /locus_tag="PLY05_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNC04615.1"
                     /translation="WTRRTERLPTDTKKLRQLLLHASDPHKVLFVDIPTLLNTSNANK
                     IAEKVSSATTQLVNAFSSRLKTIEENLLLALDHDAADWDMLNQRSKNINHVGADFKLE
                     AFISRIGSYKGQIFDIEGLLTLAIGKPTKDWTDHDLDAGEVQLLQWSNAFRRLEVLAH
                     LRNKSSSRRGIGVIFGNEHAVTGSFDVAEKDQALIENISSQLISQLKTQDYKKEVFLA
                     ALAEAGAKILQDLNQVDG"
     gene            715..>1028
                     /locus_tag="PLY05_14840"
     CDS             715..>1028
                     /locus_tag="PLY05_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HNC04616.1"
                     /translation="MAEHHVLGLSGGKDSAALAIYMRQNYPDINIRYFFTDTGKELPE
                     VYEFLAKLEGFLGKPVERINGDRDFDFWLKQFGNFLPSAQTRWCTRQLKLAPFKQWIK
                     PWL"
BASE COUNT          331 a          169 c          209 g          319 t
ORIGIN      
        1 gtggacaaga cgtactgaac gcttaccaac tgacactaaa aaactgcgac agttgttatt
       61 gcatgcctct gacccacata aagttttatt tgttgatatt ccaaccttac taaatactag
      121 caatgccaat aaaattgcag aaaaagtttc aagtgcaacc actcaattag ttaatgcgtt
      181 ctcatcaaga ctcaagacga ttgaagagaa tttacttttg gctcttgatc atgatgctgc
      241 tgattgggat atgcttaatc aacgtagtaa gaatatcaac catgtagggg ctgattttaa
      301 gttagaagcc tttattagtc gaattggctc atacaaaggt caaatatttg atatagaggg
      361 tctgttgacc ttggcaattg gtaaaccaac aaaagattgg acagatcatg atttagatgc
      421 aggtgaagta caactattac aatggtctaa tgcttttaga cgcttagaag tacttgccca
      481 tttaagaaac aaatcgtcta gcagacgtgg tattggcgtg atttttggta atgaacatgc
      541 agtaacaggc tcatttgatg ttgctgaaaa agatcaggca ttgattgaaa atatttctag
      601 ccaacttatt agtcaactta aaacgcaaga ttataaaaaa gaagtgttct tggccgcatt
      661 agcagaagct ggagctaaaa ttttacaaga cttaaatcag gtagatggtt aattatggct
      721 gaacatcatg ttttaggatt atctggcggt aaggatagtg ccgctcttgc tatttatatg
      781 cgccaaaatt atccagatat taacattcgt tattttttta cggatacagg aaaagaacta
      841 cccgaagtgt atgagttttt agccaagtta gaaggctttc ttggtaagcc tgttgaacgt
      901 attaatggtg atcgggattt tgatttttgg cttaaacaat ttgggaactt tttaccaagt
      961 gcacaaacta ggtggtgtac tcgccaatta aaactcgctc cgtttaaaca atggattaaa
     1021 ccttggct
//
LOCUS       DAOQMB010000898         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143645_length_1027_cov_14.318930, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000898 DAOQMB010000000
VERSION     DAOQMB010000898.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143645_length_1027_cov_14.318930"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1027
                     /gene="secA"
                     /locus_tag="PLY05_14845"
     CDS             <1..>1027
                     /gene="secA"
                     /locus_tag="PLY05_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252796.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; proteins in this cluster correspond SecA1;
                     SecA2 is not essential and seems to play a role in
                     secretion of a subset of proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNC04617.1"
                     /translation="TQEFRDRYTKGETLDQLLPEAFAVCREAAHRVMGMRHYDVQLIG
                     GMTLHEGRIAEMRTGEGKTLMATLASYLNTLGGQGVHVVTVNDYLASRDAELNRPLFE
                     FLGLKVGVIRSMQPTSEKHDAYLADITYGTNNEFGFDFLRDNMAFSLQEKHQRGLSFA
                     IIDEVDSILIDEARTPLIISGASEGSSQLYAAIDVIPAQLTRQKEEGKPDGSDYWVDE
                     KHRSIELTETGYENVENLLVQMGLLAEGESLYSASKLGLLHHVMSALKAHVLFQKNVH
                     YIIQDNEVVIVDEHTGRTMPGRRWSEGLHQAIEAKEGVEIQKENQTLATTTFQNYFRL
                     YQKLSGMT"
BASE COUNT          301 a          191 c          231 g          304 t
ORIGIN      
        1 agactcaaga gtttcgtgac cgctatacta agggtgaaac tttagatcaa ttattacccg
       61 aagcttttgc ggtttgtcgt gaagcggctc accgtgtcat gggtatgcgt cattatgatg
      121 tacagttaat tggtggtatg accctacatg aaggtcgtat tgccgaaatg cgtacaggtg
      181 aaggtaaaac cttaatggca acgctcgcct cgtatctaaa tactttaggc ggccaaggcg
      241 tgcatgttgt gactgttaac gattatttag caagccgtga tgctgaatta aatcgaccac
      301 tatttgaatt tttaggctta aaagtgggtg ttattcgctc catgcagcca acttccgaaa
      361 agcatgacgc ttatttggcc gatattacct atggtaccaa taacgaattc ggctttgatt
      421 ttttgcgcga taatatggcg ttttctttac aagaaaaaca tcaacgtggt ttgagttttg
      481 ccattattga cgaagttgac tcgattttaa ttgatgaggc gcgtaccccg ttaattattt
      541 caggcgcatc tgaagggtca tcgcagttat atgcagctat tgatgtgatt cctgctcagc
      601 tgactcgtca aaaagaagaa ggcaaaccag acggtagtga ttattgggta gatgaaaaac
      661 atcgctcaat cgaactaacc gaaacgggtt atgaaaatgt cgaaaatctt ttagtgcaaa
      721 tgggtttgtt ggcagaaggc gagagcttat attcggcaag caaactaggc ttattgcatc
      781 atgtgatgtc tgctttaaaa gcccatgttt tgtttcaaaa aaatgtccat tacattattc
      841 aagataatga agtagtgatt gttgatgaac atacaggccg caccatgcct ggtcgtcgtt
      901 ggtctgaagg tttgcatcaa gccattgaag ccaaagaagg tgttgagatt caaaaagaaa
      961 atcaaacctt ggcaaccact accttccaaa attacttccg tttataccaa aaattatcag
     1021 gtatgac
//
LOCUS       DAOQMB010000899         1027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143806_length_1027_cov_2.297325, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000899 DAOQMB010000000
VERSION     DAOQMB010000899.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143806_length_1027_cov_2.297325"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="PLY05_14850"
     CDS             <1..471
                     /locus_tag="PLY05_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019837005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNC04618.1"
                     /translation="QWLFNTFSWSVAFNWKGAVLASLVMSLPLMVQSIRLAIQLVDTR
                     LEDAARTLGANNWSVLFTITLPLALPGILVGALLGFSRSLGEFGATITFVGNIADETR
                     TLPLAIYSVIHQPDGEAMATRLIIISLLLAFAALLLSNYWSQRMQQRLGYQSHA"
     gene            464..>1027
                     /locus_tag="PLY05_14855"
     CDS             464..>1027
                     /locus_tag="PLY05_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018677109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNC04619.1"
                     /translation="MLELDIVIQRGDFVLPVKAQFNSTVTGIFGASGCGKSSLLTAIA
                     GLLRPQQGQIILDGQVLFDSQKKINLAPQQRHIGLMFQDGQLFPHLSVKDNLLYGYQR
                     LSRSERHFHLAEICQLLEISHLLSRLPRQLSGGEKQRVALGRAILYSPRLLLLDEPLS
                     SLDEIRKAQILPFLARVRDEVKIPMLYV"
BASE COUNT          267 a          185 c          239 g          336 t
ORIGIN      
        1 caatggttat ttaatacttt tagttggtca gtagccttta actggaaagg tgcagtattg
       61 gcctcgttgg tgatgagttt gccgttaatg gtgcagtcga tacgcttagc gattcaattg
      121 gttgatacgc gtttggaaga tgcagcacgt acgttaggag caaataattg gtcggtatta
      181 tttactatta ccttaccact ggctttaccg ggtattttag tcggggcgtt gttgggtttt
      241 agtcgtagtt taggtgagtt tggcgcaacc ataacttttg tgggtaatat tgcagacgaa
      301 acccgaacct tgccgttagc gatttatagc gtgattcatc aacctgatgg cgaggcgatg
      361 gctacccgtt taatcattat ttcgttgtta ttagcatttg ccgcgttatt gttgagtaat
      421 tattggtcgc aacgtatgca gcaacgttta ggttatcaat cccatgcttg agttggatat
      481 tgtcatacag cgtggtgatt ttgtattacc cgtcaaagcg caatttaata gcaccgtgac
      541 aggcattttt ggtgcttcgg gctgtggcaa aagtagctta ttaactgcga ttgcaggctt
      601 attgcggcca caacaaggac aaatcatttt agatggacaa gtattatttg atagtcagaa
      661 aaaaatcaac ctagcaccac aacaacgtca tattggtttg atgtttcaag atggccaact
      721 gtttccacat ttgtcagtca aggataattt attgtatggt tatcaacgct taagccgctc
      781 agaacggcat tttcatttag ccgaaatttg ccaactatta gaaattagtc atttattaag
      841 ccgtttgcca agacaattat cggggggtga aaaacaacgg gtggctttag gccgagcaat
      901 tttatattcc cctcgattat tattattaga tgagccgttg tcgtctttgg atgaaatccg
      961 caaagcacaa attttaccgt ttttggcacg agtccgtgat gaggtaaaaa ttccgatgct
     1021 ctatgtc
//
LOCUS       DAOQMB010000900         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_143924_length_1026_cov_16.839341, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000900 DAOQMB010000000
VERSION     DAOQMB010000900.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_143924_length_1026_cov_16.839341"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLY05_14860"
     CDS             <1..274
                     /locus_tag="PLY05_14860"
                     /inference="COORDINATES: protein motif:HMM:NF018009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HNC04620.1"
                     /translation="DLVFYNYLLKCFLLVDLKVGKLTHQDVGQMDMYVRVFEEQYRGE
                     GDNPTLGLILCSERNEAVAKYSLVVIVHLYLPRPSYRQSYNVTVPC"
     gene            complement(416..775)
                     /locus_tag="PLY05_14865"
     CDS             complement(416..775)
                     /locus_tag="PLY05_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNC04621.1"
                     /translation="MKTLLQQLKERRLEVGFKQNDMLLRVGMSRQQYQHLESKGNPRL
                     NTLELLAKGLNSELMLIPKEKLTAVLAILEQSPHHKPTDNHVNIKKTLSHSTLEEANS
                     GSALADNPWQFLFGDNE"
BASE COUNT          280 a          174 c          225 g          347 t
ORIGIN      
        1 cgatttggtt ttttacaatt atttgctcaa gtgcttttta ttggttgacc taaaggtggg
       61 taaacttacg catcaagatg ttggtcagat ggatatgtat gttcgtgtct ttgaggagca
      121 ataccgaggc gaaggcgata atccaacatt aggcttaatt ctttgttctg agcgtaatga
      181 ggctgtggcc aagtattcgc tagtcgttat cgttcatttg taccttccga ggccgagtta
      241 caggcagagt tacaacgtga ccgtgccgtg ttagaaaatg ccagtccagc aggtgatgta
      301 cagtaagcga tattcaagcg ttaattactg agcgtgttac ggccatgttg aaagccgact
      361 aaataagcaa tcacttggcc atgtaagcta agctgaagaa tattgacctt gtcactcact
      421 cattatctcc aaacaaaaat tgccaaggat tatctgcaag tgcgctgcca gaattagctt
      481 cttcaagcgt tgaatgacta agcgtcttct taatattgac gtgattatct gttggcttat
      541 gatgcggtga ttgctcaagt atggctaaaa ctgccgtgag cttttcttta ggaatgagca
      601 tcaattcact attcagccct ttagcaagca actcaagggt atttaatcga ggatttcctt
      661 tggattctag atgttgatat tgttgacgtg acatacccac acgcaacagc atatcgtttt
      721 gtttaaagcc cacttcaaga cggcgttctt tgagttgttg cagtaaagtt ttcatagact
      781 tgtcaacaaa taagttgctt tttttgatga tggaataata gtagtgtgac ttttctacaa
      841 aagcaattta tatattgctt ttttgatggc cttgtgtttt gtgggcttaa agtgttactg
      901 agcttgagtt ggttggcgaa aggaatgagt cgattctatt ctattacatg tagatgctta
      961 ttaatattta tcatgttttt agcgcgaaaa acacgataaa tgaattgctt atttttttag
     1021 aaaaat
//
LOCUS       DAOQMB010000901         1026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_144045_length_1026_cov_2.714727, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000901 DAOQMB010000000
VERSION     DAOQMB010000901.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144045_length_1026_cov_2.714727"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..>1026
                     /locus_tag="PLY05_14870"
     CDS             51..>1026
                     /locus_tag="PLY05_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNC04622.1"
                     /translation="MTTNNTTSNESTAKFKLNSLQLSGFKSIAATGQKIDFGDVTVLL
                     GANGCGKSNLISFFKMLNHIVNDNLPSYVGKQGGTDALLFYGSAITDKIDFVLNFSQR
                     QADTPAFSIEYQASLQFGMPDRLFFAREVISRGSQGYPPLDISVLHSGNSTSAIFKPS
                     TDPRLLNAFASINTYQFHDTSDTAKIKRNSFKDDAHYLREDAANLAAFLRVMKETPSK
                     KKYYQRIVRHIQHVMPQFGEFDLQLLADNNNYVRLNWREKGGDYLFGAHQISDGSLRF
                     MALATLLLQPPDTLPSVIVLDEPELGLHPAAIAELAAMIRIASRYSQLI"
BASE COUNT          293 a          230 c          205 g          298 t
ORIGIN      
        1 ggtgcagccc ttaaaaagat tgtaggctta gatacaggcg atgattaacc atgaccacca
       61 ataacacgac aagcaacgaa tcaacggcta aatttaagct aaatagctta caactcagtg
      121 gctttaagtc gattgctgca acaggtcaaa aaatcgactt tggtgacgtg acggtattat
      181 taggcgcaaa tggttgtggc aaaagcaatt taatttcatt ttttaaaatg cttaatcata
      241 tcgttaatga taatttgccg tcctatgtag gcaaacaagg cggtactgat gcgttattgt
      301 tttatggctc agccattacc gacaaaatag actttgtgct taattttagc caacgccaag
      361 ctgatacacc tgctttttct attgagtacc aagccagctt acaatttggt atgcctgacc
      421 gtttgttttt tgcaagggaa gtgatttcgc gtggttcgca aggttatcca cctttagata
      481 tttcggtttt acattcggga aatagcacat cggccatttt taaaccatcc accgatccac
      541 gtttgctcaa tgcgtttgcc agtataaaca cctatcaatt tcacgacacc tccgacactg
      601 ccaaaatcaa acgtaatagc tttaaagacg atgctcatta tttacgcgaa gatgccgcca
      661 acttagctgc atttttacga gtgatgaaag aaacaccgag caaaaaaaaa tactatcagc
      721 gtattgttcg tcatattcaa cacgttatgc cgcagtttgg cgagtttgac ttgcagcttt
      781 tagccgataa caacaactat gttcgcctta actggcgcga aaaaggcggt gactatttgt
      841 ttggcgcaca tcaaatttcg gatggttcgc tacgatttat ggccttagcc accttactat
      901 tgcagccgcc cgatacttta cccagcgtga ttgttttaga cgaacccgaa ttagggctgc
      961 atcctgccgc gattgccgaa cttgcagcca tgattcgcat cgcctcacgc tatagccagt
     1021 taatta
//
LOCUS       DAOQMB010000902         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_144534_length_1024_cov_10.955624, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000902 DAOQMB010000000
VERSION     DAOQMB010000902.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144534_length_1024_cov_10.955624"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..326
                     /locus_tag="PLY05_14875"
     CDS             60..326
                     /locus_tag="PLY05_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004994782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HNC04623.1"
                     /translation="MHNQTIAMERINLRASAQAKQVIEQAAQLMGTNLSAFMLNQSYE
                     AALRIINQHQVWLLANADRDALLDALDNPQEPTQALRDLMALSK"
     gene            323..820
                     /locus_tag="PLY05_14880"
     CDS             323..820
                     /locus_tag="PLY05_14880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010325589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04624.1"
                     /translation="MTQYVIALLDKHHQRKTFDCGQEDLNNFIQLYALQQQKRQQNKT
                     YVAYTPEQQIIGFYSLNVGAIAFEEMPAEIQKRLARYPIPTVYIGRFAVDVNHQGKGL
                     GKVLLSHALHRVCEIAAIAGVTVVAIDAKNQQAAQFYQTMGFRALPSQPLKLFLPVTE
                     LLKVL"
BASE COUNT          306 a          186 c          216 g          316 t
ORIGIN      
        1 cttatgttga ttgtttttga ctataatgta cataatattt atgtacatag ggtcttgtca
       61 tgcacaatca aactattgcg atggagcgta ttaatttacg cgcatctgct caagctaaac
      121 aagttattga acaagcggct caactgatgg gtacaaattt gagtgccttc atgcttaatc
      181 aatcatacga agcggcttta cgaattataa accaacatca agtgtggcta ttagcgaatg
      241 ccgacagaga tgctttgtta gacgcgttag ataatcccca agaaccaaca caagctttgc
      301 gtgatttgat ggcgttatct aaatgacgca atatgttatc gctttgttag ataaacacca
      361 tcaacgtaaa acatttgact gtgggcagga ggatttaaac aattttattc agctttatgc
      421 tttacaacaa caaaagcgac agcaaaataa aacttatgtg gcttatacac cagagcagca
      481 aattattggt ttttatagtc ttaatgttgg cgcgattgct tttgaggaga tgcctgctga
      541 gatacaaaag cgtttagcac gttatcctat tccaacggta tatattggac gttttgcggt
      601 tgatgttaat catcaaggaa agggattggg gaaggtgtta ttgtctcatg ccttgcatag
      661 agtttgtgag atagctgcaa ttgctggtgt gacggtagtt gccattgatg caaaaaatca
      721 acaagcagca cagttttatc agactatggg ttttagggca ttgcctagtc agcctttaaa
      781 attatttttg cctgtcacag agttgttaaa agttttgtag cgtgtggttg aggaaaggaa
      841 acccacaaca attcaatcgt ggctttcttt gccaaactat tctataaatt tgctaactgt
      901 actcgtccat ctttcacagg cggacaccaa aaataagcgc ctgttattgg tcgtgtaaat
      961 tgaaatagcg catcttgaat gccatcttca aggccaacca tacgccgtaa ttgtgccgca
     1021 aatg
//
LOCUS       DAOQMB010000903         1024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_144584_length_1024_cov_3.692466, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000903 DAOQMB010000000
VERSION     DAOQMB010000903.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144584_length_1024_cov_3.692466"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1024)
                     /locus_tag="PLY05_14885"
     CDS             complement(<1..>1024)
                     /locus_tag="PLY05_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002116724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNC04625.1"
                     /translation="NKHLIDENSLRRYDLNIVKHWQQITERRNYHEGVTLHLKYFQYL
                     SLLVAEIYLDYYVNKPQEMLVGLNEQLTQYNADKHIDQQFERYSQDDLNKVAYWSATG
                     SGKTLLLHVNILQYLHYFKQKNGDKAHPDKIILLTPNEGLSHQHFKELQESGFEATFF
                     DKNKIGQGSLDREVIQIIDINKLADEMGDKTVAVEAFTGNNLVLVDEGHRGAGTAAGA
                     WMSRRETLSKGGFSFEYSATFGQAIGKGDNVYKATLEAQKKKAKMLGTEKADIIKLEL
                     DDLEKQKVKQQALREVYAKSILFDYSYKYFYQDGYGKESLILNLQEAQYSDKRDLYFT
                     ACLLAFY"
BASE COUNT          276 a          215 c          185 g          348 t
ORIGIN      
        1 ataaaaagct aacaaacagg ccgtaaagta caaatctcgc ttatcgctat attgagcctc
       61 ttgcaagtta aggatcaatg actctttgcc atagccgtct tggtaaaaat acttgtagga
      121 ataatcaaac aaaatgcttt tggcatacac ttcacgcaag gcttgctgtt taactttttg
      181 tttttctaaa tcatctaatt ctagtttgat gatgtcagct ttttctgtgc ctagcatttt
      241 ggcttttttc ttttgtgctt ctaaggtggc tttatacaca ttatcacctt tgccaatcgc
      301 ctgaccaaaa gtagccgaat actcaaacga aaaaccgcct ttgctcagcg tttcacggcg
      361 gctcatccat gcccctgcgg ctgtgcctgc acctctatga ccttcgtcca ccaacactaa
      421 gttattgcca gtaaaggctt caacagcaac agttttgtcg cccatttcgt cagccagttt
      481 attgatgtca ataatttgaa tcacttcgcg gtctaagctg ccttgtccta ttttattttt
      541 gtcaaaaaag gtagcttcaa aaccagactc ttggagttct ttaaaatgtt ggtgtgataa
      601 gccttcgttg ggtgtcagca aaatgatttt atcgggatgt gctttgtcgc catttttttg
      661 tttaaagtaa tgcaaatact gcaagatatt aacatgcaac aataaggttt taccgctacc
      721 tgtggcactc caatacgcta ctttgtttaa gtcatcttgg ctatagcgtt caaattgctg
      781 gtctatatgt ttatcagcat tatattgagt gagttgttca tttaagccta ccaacatctc
      841 ttgcggctta ttaacataat aatccaaata aatctcggcg actaacagcg ataaatactg
      901 aaagtatttt aggtgcaggg taacgccttc gtgatagtta cgccgctcgg taatttgttg
      961 ccaatgttta acgatgttta ggtcataacg tcttaagcta ttttcatcaa tcagatgctt
     1021 atta
//
LOCUS       DAOQMB010000904         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_144821_length_1023_cov_7.372934, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000904 DAOQMB010000000
VERSION     DAOQMB010000904.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144821_length_1023_cov_7.372934"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLY05_14890"
     CDS             complement(<1..857)
                     /locus_tag="PLY05_14890"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNC04626.1"
                     /translation="MPTLQQIWPATPSIYANMTVTGLTANSRHVQQGDVFVALQGRQH
                     DARHFIPQAIAQGAVAVLCQSDEPSTEQSGIPIISIPDLPQHLGQIAACFYSQPSQDL
                     TVFAVTGTNGKTSCAQLLAHACQFLGQKSAVLGTLGNGLVGELVPSTHTTLDALQLQQ
                     KLAEFKQAGAQVVALEASSHGLEQGRLSATKIDTAIFTNLTRDHLDYHGTMEAYQQAK
                     ALLFQWPTLKTAILNADDPVSKDYQQLLTPNVICWRYSQNANSDAEFVALKIEPSLQG
                     LIVTVKTPQG"
BASE COUNT          273 a          218 c          229 g          303 t
ORIGIN      
        1 ccttgaggtg ttttaacggt gactatcagc ccttgtaatg atggttcaat ctttaaggca
       61 acaaattcag catcactatt ggcattttga ctataacgcc agcaaatgac attgggagtt
      121 aataattgtt gataatcttt agaaactggg tcatcggcat taagaatggc cgtttttaag
      181 gttggccact gaaataataa ggctttggct tgctgatagg cttccatcgt gccgtgataa
      241 tctaaatggt cacgggttag attggtaaaa atagcagtat caatcttagt tgccgataaa
      301 cggccttgct ctaagccatg tgagcttgcc tctaaggcaa ccacttgtgc gcctgcctgt
      361 ttaaattcgg ctagtttttg ctgtaattgc aaggcatcta aggtggtgtg cgttgacggg
      421 actaactcac ccactaaacc attacccaat gttcctaaaa cggccgattt ttgccctaaa
      481 aactggcaag cgtgcgctaa taattgtgcg caactggttt taccattagt acctgttact
      541 gcaaaaacgg ttaaatcttg gcttggctga ctgtaaaaac acgctgctat ttgccctaaa
      601 tgttggggta aatcgggaat actaatgata ggaatacccg attgctcggt tgatggctca
      661 tcactctgac acaacaccgc caccgcacct tgtgcaatcg cttgcggaat aaaatgtcgt
      721 gcatcgtgtt gacgaccttg taatgccaca aaaacatcac cttgttgtac atggcgacta
      781 ttggctgtta aacctgtaac cgtcatattg gcatagatac tcggcgtagc tggccaaatt
      841 tgttgtaatg tcggcattta aacacgtcct ccctgaacga tggtattggt ttttacaggt
      901 gtaatcggca ctgttgttag cgtaggtgtt ttagttttta caggctcaag tggtttatcg
      961 acaggcacat tcattaatcg taacgcctca cccatcacgc gtgaaaaaac aggtgcagag
     1021 act
//
LOCUS       DAOQMB010000905         1023 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_144825_length_1023_cov_6.511364, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000905 DAOQMB010000000
VERSION     DAOQMB010000905.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_144825_length_1023_cov_6.511364"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1023
                     /locus_tag="PLY05_14895"
     CDS             <1..>1023
                     /locus_tag="PLY05_14895"
                     /inference="COORDINATES: protein motif:HMM:NF037897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04627.1"
                     /translation="LLTEQEKAQFATPQERNAVQRLIDHIVRAVKGWVVRHLSKYNAT
                     AGIGKRVSETLDARDIQLMAERMIRDIAKYSGDNNRPKFSKTEQSQFDQLLAKADKMI
                     AEQGKLLAPNGKPSKLNRYQWAQVRTDNFKQWFGDWENDTREEKGRYDIGGVVGVSAE
                     ANPRTGGNAEQMGRADSRVRLDGFGRGRALLDATTREPKVFFHGTKDDIEAFDLNHPN
                     RKDIGWLGRGVYVASDARLANAYAKLKRGDGDENVMPVFVSVKNPYFATTQEKQSLKF
                     ATQAHADLVTQRAKERGHDGMVVQFPDGTIELVAFDPTQIKSATSNNGEFNPTNPDIR
                     FSQKNN"
BASE COUNT          341 a          212 c          239 g          231 t
ORIGIN      
        1 atttattaac cgagcaagag aaggctcaat ttgccacacc acaagagcgt aatgccgtac
       61 aacgcttaat tgaccatatt gtacgcgccg ttaaaggctg ggtagttcgt catttaagca
      121 aatacaacgc aaccgcaggc ataggcaagc gcgtgtctga aacgcttgat gcgcgtgata
      181 ttcagttaat ggctgaacgg atgattagag atattgcaaa gtatagtggt gacaataata
      241 ggcctaaatt cagcaaaacc gaacaatcac agtttgacca attacttgcc aaagctgaca
      301 aaatgattgc cgagcaaggc aaactacttg cacctaatgg taaaccctct aaactaaacc
      361 gttatcagtg ggcgcaagtg cgtaccgata actttaaaca gtggtttgga gattgggaaa
      421 atgacacaag agaagaaaaa ggaagatatg acattggagg agttgttgga gtctctgccg
      481 aagctaaccc ccgaacagga ggcaatgctg aacaaatggg gcgagccgat agcagagtac
      541 ggcttgatgg atttggacga ggacgagcgt tactggacgc aacaacaaga gaaccaaaag
      601 tcttctttca cggcacaaaa gacgacatcg aagccttcga cttaaatcat cctaatcgta
      661 aagatattgg ttggttgggg cgtggcgtgt atgtcgcaag tgatgcgcga ctggcaaatg
      721 cttatgctaa gttaaaacgt ggtgatggcg atgaaaacgt catgcctgtt tttgtgtcgg
      781 ttaaaaatcc atattttgcg acaacacaag aaaagcagag ccttaaattt gccactcaag
      841 cacacgcgga tttagtcact caacgcgcaa aagaacgcgg tcatgatgga atggttgtac
      901 aattccctga tggaacaata gaattagtcg cttttgaccc cacacaaata aaatccgcaa
      961 catcaaacaa tggcgaattt aacccaacca atcccgatat tcgctttagt caaaaaaaca
     1021 atg
//
LOCUS       DAOQMB010000906         1022 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_145078_length_1022_cov_30.656670, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000906 DAOQMB010000000
VERSION     DAOQMB010000906.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145078_length_1022_cov_30.656670"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(28..924)
                     /locus_tag="PLY05_14900"
     CDS             complement(28..924)
                     /locus_tag="PLY05_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HNC04628.1"
                     /translation="MDKLTELFCLVDNFCQVFEPEWNKHLLQSGHKQRHKQTQLSLSE
                     MMTIVILFHQLRYRHFKVFYQDYVLAYLAPYFPKLLSYNRFVELMPRCLVGLTAFSQT
                     LKGECTGISFVDSTPLKVCHNRRIPRHKTFVNYAARGKNSIDWFYGFKLHLLINHQGE
                     LVCFKLTSGNVNDRHPIKAFCQKLHGLLFADKGYLGQAITEQLADMGVKLITPVRKNM
                     KEKMYTAFEKCLLQRRGVIETVNHELKNLCQIEHTRHRSLNNFLVNLMAGIIAYCLQP
                     KKPTVKGLKKNQSLLKTVKSIC"
BASE COUNT          310 a          190 c          189 g          333 t
ORIGIN      
        1 ttaacctgag ttcgggataa tgtaccttta acatattgat ttaactgttt ttaataaaga
       61 ctgattcttt ttcaagcctt tgactgttgg ttttttgggc tgtaaacagt aagcgataat
      121 gcccgccatc aaattgacta aaaaattatt gagagaacga tgacgggtat gttcaatttg
      181 acacaagttt tttaattcat gattgaccgt ttcaataacg cctctacgtt gtaaaagaca
      241 tttttcaaaa gccgtgtaca ttttttcttt catattttta cgaacaggtg taattaattt
      301 aacacccata tcggctaatt gttcggtgat ggcctgtcct aaatatccct tatcagcaaa
      361 taataaaccg tgtaattttt ggcagaatgc tttgatagga tgacggtcat tgacattacc
      421 tgaggtcagc ttaaaacaga ctaactcccc ttgatgatta atcagtaaat gcagcttaaa
      481 gccataaaac caatcaatgc tatttttacc tctagctgca taattcacaa aggttttatg
      541 tcgtgggata cgtctgttat gacatacttt taagggggta gaatccacaa aactaatgcc
      601 tgtacattcg cctttgagcg tctgtgaaaa tgccgttaat cccaccaaac agcgtggcat
      661 caattcaaca aatcggttgt aggatagcag ctttggaaaa tagggggcta gataagcaag
      721 tacataatct tgatagaaca ctttaaagtg acgatacctt aattgatgaa acaaaatcac
      781 gattgtcatc atctcagaca ggctaagttg cgtttgtttg tgacgctgct tatgtccgct
      841 ttgtagtaaa tgcttattcc attcgggctc aaaaacttga caaaagttat cgacaagaca
      901 aaaaagttcg gtaagcttat ccatgggtga gcctttagac agttaaagtg tttgtgtctg
      961 aaccaaaact ttactctgtt ttatcggctc attcttcctt ttcttagccc gaactcaggt
     1021 ta
//
LOCUS       DAOQMB010000907         1021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_145384_length_1021_cov_6.594203, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000907 DAOQMB010000000
VERSION     DAOQMB010000907.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145384_length_1021_cov_6.594203"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="PLY05_14905"
     CDS             complement(<1..973)
                     /locus_tag="PLY05_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04629.1"
                     /translation="MARAFANDFAHDGVFNAVGSQALAVNYSNAFLTHWVNWVSNFYA
                     SFLNLQTIVDFNRWYGGFNIANKESVKAYPDAVPIRTVDGVEEYACSGEGRIKSSQSD
                     GISMDFVNQRGSNMSIYWLDYSAKRVSYKQNLPTGQTHVQGTFVTHPWLITDNTGTCI
                     GIYRPVTKTNKTLTFKSNEVLLGNGNSQATSCTTGVETTFATTATNAPFTNGQKVCFD
                     ATTTTLAFNSKTLSNPVKNTVVQLPYAAYSFKDGSYSYEVIFNNNALYEINLSQASFL
                     GQFTPLTTTPTVVTCASKNLPVATLSSLADYNGDYKDAGTVVFNLNTS"
BASE COUNT          311 a          199 c          193 g          318 t
ORIGIN      
        1 tgctggtatt taagttaaat acaacagtcc ctgcatcttt ataatcacca ttatagtccg
       61 caaggctgct taatgttgcc acaggtagat ttttgctagc acaggttact acggtaggtg
      121 ttgttgtcag aggtgtaaat tggccaagaa atgaagcctg cgacaagtta atttcgtaaa
      181 gtgcgttatt gttaaaaata acttcgtaac tataactgcc atctttgaag ctatatgcag
      241 cataaggaag ttgcacaacc gtatttttaa cagggttact tagggttttg ctgttaaacg
      301 ccagagttgt agttgtcgca tcaaaacaaa ctttttgtcc attagtaaaa ggagcattgg
      361 ttgctgttgt tgcaaatgtc gtttcaacac ccgttgtaca acttgttgct tggctattac
      421 cattacccaa aagtacttca ttgcttttaa acgttaaagt tttattggtt ttagtgacgg
      481 gacgataaat accaatacaa gtgcctgtat tatcggtaat taaccaaggg tgtgtaacaa
      541 atgtaccttg cacatgggtt tgacctgtgg gtaaattttg tttataggaa acacgttttg
      601 cactataatc caaccagtaa atactcatgt ttgagccgcg ttggttgaca aaatccatcg
      661 agataccatc actctgacta gatttaattc gtccttcgcc actacacgca tattcttcaa
      721 caccatctac ggtacggatt ggcacagcat caggataggc tttaacactc tctttatttg
      781 caatgttaaa tccaccatac caacggttaa aatcaacaat ggtttgtaaa tttaaaaatg
      841 acgcataaaa attagacacc caattaaccc aatgagttaa aaaggcattg ctataattaa
      901 cagctaaggc ttggctgcct acggcattaa acaccccatc atgggcaaaa tcattagcaa
      961 aagcacgagc catatcataa gcaggtgttg agctagtgct attaatcctt tgtgcttctt
     1021 t
//
LOCUS       DAOQMB010000908         1020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_145690_length_1020_cov_17.158549, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000908 DAOQMB010000000
VERSION     DAOQMB010000908.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_145690_length_1020_cov_17.158549"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(198..>1020)
                     /locus_tag="PLY05_14910"
     CDS             complement(198..>1020)
                     /locus_tag="PLY05_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04630.1"
                     /translation="LTLRKMIAVLSLSWLGMAGHTATLSTNEQQKLDSIVTQLNQLPK
                     IDNKYSQQAKEQALTIIAQAVKANPNLAATLTEFAIKQGAGVSATVKALAKVAPKQAG
                     AIVTAAIKASKPSMAAAIAAAALSVAPKQAAAITQAAITASPKHAAAITSAGISTAPS
                     QTKTILQTAFKMGNSSEDRRKVAIAADNAGVKETVINQLLREMTINNKGQYEVFKHNQ
                     KNTSDSIISIGSINDPSSAIGVSQGQGVNISLNNQTTGSTGGGGGCQKTLTATCS"
BASE COUNT          256 a          197 c          204 g          363 t
ORIGIN      
        1 gttaaataac caccatcttt ttgactgatt aggcctttac tatgcaaacg ttcagcagca
       61 gcaataataa ttggttctgc actatgatga atttttaaac ccgcttgatg attagctaag
      121 tcaaacatcg ctaatacggt taattcttct attaaatcaa tggaatattt cattgttatt
      181 ctccgttata ttatttatta agaacaagtt gctgttaatg ttttttgaca tccacctcca
      241 ccacctgtag aacccgttgt ttgattattt aaggaaatat tgacaccttg tccttgtgat
      301 acacctatgg cggatgatgg gtcattgatt gaaccgatac taattataga gtcactggta
      361 tttttttgat tgtgtttaaa gacttcatac tggcctttat tattaatggt catttcgcgc
      421 aataattgat taatcaccgt ttcttttacg cctgcattat cagcggcaat cgctactttg
      481 cggcgatctt cactggaatt acccatttta aatgctgttt gtaaaatggt cttggtttga
      541 ctaggtgcag tgctaatgcc tgcagaggta atcgccgctg catgtttagg cgatgctgta
      601 atagctgctt gcgtaattgc ggctgcttgt ttgggtgcaa cacttaaagc ggctgcggca
      661 atcgctgccg ccattgacgg ttttgatgct ttaatagctg ccgtcacaat cgctcctgct
      721 tgtttgggtg caactttagc cagtgctttt accgtagcag acacgcctgc accttgttta
      781 atggcaaact cggttaacgt ggctgctaaa ttaggattgg cttttactgc ttgagcaata
      841 atagttaaag cttgttcttt agcttgttga ctatatttat tatcaatttt gggtaattga
      901 ttcagttgag taacaataga gtctaacttt tgttgttcat tggtggataa agtcgctgta
      961 tgccctgcca tgcctaacca tgataagctt aaaaccgcta tcatttttcg caaggtcaat
//
LOCUS       DAOQMB010000909         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146256_length_1018_cov_15.398754, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000909 DAOQMB010000000
VERSION     DAOQMB010000909.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146256_length_1018_cov_15.398754"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..>1018
                     /locus_tag="PLY05_14915"
     CDS             234..>1018
                     /locus_tag="PLY05_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005401468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNC04631.1"
                     /translation="MADLPLLLDAQQLLDNLHHPELLIVDLCKKSVYQQAHIPNAVWV
                     NTRDLVSGELPATGKLPSVEQLQQLFANIGLTANKHLVVYDDEGGAWAGRFIWTLDII
                     GHKNYSYLNGGLHAWLAAGFAIDNSPTIPTTSSPSINFNEQPQALLADVLAHYQDSNY
                     VLWDARSVEEYTGSRLLAMRGGHIPNAVHYEWNQVLDKENSLKLRVLNEVKQELHHLG
                     ITANKTVIVYCQTHHRSGLSYLVGKLLGLNIKAYAGSWSEWGN"
BASE COUNT          308 a          197 c          196 g          317 t
ORIGIN      
        1 acaggaatgc ctttgggatt accactttct tcaatatata aatgatgtgt gtcatccact
       61 tgtaagtgaa attgatgata aggctgaatt tctggataga gtgttaacat aacgattccc
      121 taaagtattt ttttcaagaa tactggattt tttaacaaat aaccaaactc tgttttgctt
      181 aacttgttca cagtgtttac aattggccta gaatttttat cgcgagacca acaatggccg
      241 acttgcccct gttacttgat gctcagcaac tacttgataa ccttcatcat cctgaattat
      301 taattgtcga tttgtgcaaa aaatcggttt atcaacaagc acatattcct aatgcggtat
      361 gggttaatac ccgcgattta gtgtcaggtg agctaccagc aacaggcaaa ctaccttcag
      421 tagaacaatt acaacaactg tttgccaata ttggtttaac ggcaaataaa catttggttg
      481 tttatgatga tgaaggcggc gcatgggctg ggcgttttat ttggacatta gatattattg
      541 gccacaaaaa ctattcctat ttaaacggcg gtttacatgc gtggttagcc gcaggttttg
      601 ctattgataa tagtccaaca atacctacaa ctagcagccc aagtattaat tttaacgaac
      661 aaccacaagc tttattagct gatgttttag cccattacca agactcaaac tatgtattat
      721 gggatgctcg ctctgttgaa gaatatacag gctctcgtct actcgccatg cgtggcggcc
      781 atatccctaa tgctgttcat tatgaatgga atcaggtact tgacaaagaa aactcactta
      841 aacttcgagt cttaaatgaa gttaaacaag agttgcacca tttgggcatc accgccaata
      901 aaacggtgat tgtttattgc caaacccatc atcgttcagg tttaagctac ttagtcggaa
      961 aactcttagg gctgaatatc aaagcttatg caggttcatg gagtgaatgg ggaaattt
//
LOCUS       DAOQMB010000910         1018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146257_length_1018_cov_13.986501, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000910 DAOQMB010000000
VERSION     DAOQMB010000910.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146257_length_1018_cov_13.986501"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLY05_14920"
     CDS             complement(<1..370)
                     /locus_tag="PLY05_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNC04632.1"
                     /translation="MSATLDLTLDLIRCPSVTPVDANCQELMIQRLEKIGFHIERLRF
                     GEVDNFWARRGTTAPVFAFAGHTDVVPTGTLTAWLNDPFQPEIRDGVLYGRGAADMKG
                     SLAAMVVACENFVAQHPNHQG"
     gene            complement(445..>1018)
                     /locus_tag="PLY05_14925"
     CDS             complement(445..>1018)
                     /locus_tag="PLY05_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HNC04633.1"
                     /translation="RFKNTTNSDHQKPVYDNHIKQHFEVERPNRVWSCDITYVLTDEG
                     WLYVAGVKDVYTKELVGYCLSSRMTADIVVQALTMAIKRRKPTKGLIVHSDRGSQYCS
                     HAYRDLIAQHEFVGSMSAKGNCYDNAPIESFWATLKNELVYHQHYATRMEAIKDIQYF
                     IEIFYNRMRIQAKLNFRSPAQAFEQFKRAT"
BASE COUNT          305 a          228 c          196 g          289 t
ORIGIN      
        1 acccttgatg attcggatgt tgggcaacaa aattttcgca agcaaccacc atcgcggcca
       61 acgaaccttt catatctgcc gcaccacgac catataacac accatcacga atttcgggtt
      121 gaaaagggtc attaagccaa gcagttaatg tgcctgtggg aacaacatcg gtatggccag
      181 caaaggcaaa gacaggtgcg gttgtaccac gacgtgccca aaaattatcc acctcaccaa
      241 aacgtaaacg ttcaatgtga aagccaattt tttctaaacg ttgaatcatt aattcttggc
      301 aattggcatc aacaggcgtg acggaagggc aacgaatcag gtctaaagtt aaatctaaag
      361 tagcagacat aatatttcca aataagttag cttgtatctt gttgatatgt gttgagttta
      421 gcgtatgact ggacttggga taaattaagt tgccctctta aactgttcaa atgcttgagc
      481 tggcgaccta aaatttagtt tagcttgaat tcgcattcga ttgtagaaaa tctcaataaa
      541 atattgaata tccttgatgg cttccatacg cgttgcatag tgctgatggt aaaccaactc
      601 gttttttaac gttgcccaaa agctttcaat gggcgcatta tcataacaat tccccttagc
      661 actcattgaa cccacaaatt catgttgcgc gattaaatca cgataagcat ggctacagta
      721 ttgactgcct ctgtcagaat gaacaattaa gcctttcgtc ggcttgcgcc gtttaatcgc
      781 cattgttaat gcctgcacca cgatatcagc cgtcattcga gaagataggc aatagcccac
      841 caattctttc gtatacacat ctttcactcc agccacatat aaccaacctt catcagttaa
      901 gacataagta atgtcacaag accataccct attagggcgt tccacctcaa agtgttgttt
      961 gatatgattg tcatacacag gcttttgatg gtcgctattc gtggtgtttt taaagcgt
//
LOCUS       DAOQMB010000911         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146548_length_1017_cov_15.839917, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000911 DAOQMB010000000
VERSION     DAOQMB010000911.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146548_length_1017_cov_15.839917"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1017)
                     /locus_tag="PLY05_14930"
     CDS             complement(<1..>1017)
                     /locus_tag="PLY05_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04634.1"
                     /translation="SQGFVAITDIEPTNPSDNVGSKIKTDDNNVLQSCISSTPNITVS
                     VLAVTAGQSFKPSVTVNGVAATLSRNALTDVWTGSAAITLTGEAPYTVTATHSEGATD
                     TAIVNMEALPVVTNMQFSGAYPNVGQTEHAQGQTLSLTITSDTQFTELEIVDDAGTAT
                     EALSLSFAATTTKTVTVTVADRGSYGTGAPIIVPAKARIKSANGTWGNYFESNDFGGT
                     NGVHILALNNTRPLVEINSIVYPVGQYALKDTESVTVNVSESNVDSLVWSSPNSQLSI
                     SSPSTSGNKTVTRASGGYNITDNNLRVVSNRVANATQTTLDAIVWIAHDAPTITVTTP
                     AARL"
BASE COUNT          265 a          224 c          207 g          321 t
ORIGIN      
        1 gtaatcgtgc ggcaggcgtt gtgactgtga ttgtaggcgc atcgtgagca atccaaacaa
       61 tcgcatctaa tgttgtttgt gttgcgtttg ccacacgatt tgatacaact cgcaaattgt
      121 tatcagtgat attgtaccca cccgatgcgc gtgtgactgt tttatttccc gatgtgcttg
      181 gactgctaat agataattgg ctgtttggcg aagaccacac caaagaatca acattgcttt
      241 cagatacatt aactgtgact gactctgtat cttttaacgc atattgaccc actggataaa
      301 caattgaatt aatctcaacc aacggacgtg tattattcaa agcaagaata tgcacaccat
      361 ttgtgccgcc aaaatcattg ctttcaaaat aatttcccca tgtgccgttt gcacttttaa
      421 ttcgagcttt tgctggaaca atgatgggtg cgcctgttcc gtagctgcct ctatcggcga
      481 cagttaccgt tactgtttta gtggttgtgg ctgcaaacga taagcttaac gcttctgttg
      541 ccgttcctgc atcatcaaca atttctaatt ctgtaaattg cgtatctgat gtaatcgtta
      601 atgacagtgt ttgaccttgt gcgtgttctg tttgaccaac attaggatat gcaccactaa
      661 attgcatatt agtaacaact ggcaatgcct ccatattaac aatagctgta tctgttgctc
      721 cctcgctgtg tgtggccgtc actgtataag gagcttcgcc tgttaaagta attgctgctg
      781 aaccagtcca cacgtcagtt aaagcgtttc gtgataatgt agcagccaca ccattaaccg
      841 tgacacttgg cttaaaactt tgccctgcgg tcacagcaag cacagaaaca gtaatgtttg
      901 gtgttgagct aatacaagac tgcaacacat tgttatcatc tgtttttatt ttagacccta
      961 cattgtcact tggatttgta ggctcaatat cggtaatcgc cacaaatcct tgagagc
//
LOCUS       DAOQMB010000912         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146564_length_1017_cov_5.248441, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000912 DAOQMB010000000
VERSION     DAOQMB010000912.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146564_length_1017_cov_5.248441"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLY05_14935"
     CDS             <1..236
                     /locus_tag="PLY05_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04635.1"
                     /translation="QLQTLLAYQLRQQGLKDADKPEWIAKQMLQVSGATFNTENNYNC
                     PHDLTTRLANTLAVAEFLGRAQRQANPKQGEQQ"
     gene            233..>1017
                     /locus_tag="PLY05_14940"
     CDS             233..>1017
                     /locus_tag="PLY05_14940"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HNC04636.1"
                     /translation="MSLQTRLIKPLDVLMLRGNQSFGEAGEHGKSSMPPSPSVLSGAL
                     RSFWLAQSNIDMQKFAKMSPSDLKEPLCSQLGTPSQTGSFRLSDCGVVRKNSNNYERL
                     YPLPSDLVLQKLPKDNDSLTPYALQPQTLHNDLMGCHNGQLAILKAPAGKPEGGYWLT
                     ESGFNSYLKGQLPTIKDLIKTSELWKSDWRLGIALNSESRTASEGQLYTTEAIALQKD
                     VFLMVSIAGANDFPKEGTLRLGGDGRAAGFESVALSQLDTIQAT"
BASE COUNT          332 a          220 c          202 g          263 t
ORIGIN      
        1 gtcaattaca aaccttatta gcctatcaac tgcgtcaaca aggtttaaaa gatgccgaca
       61 aacccgaatg gatagccaaa caaatgctac aagtatcagg tgcaacattt aacacagaaa
      121 ataactataa ctgcccacat gatttaacta cacgccttgc taatacttta gcggttgctg
      181 aatttttagg tcgcgctcaa cgtcaggcaa atcccaaaca aggagaacag caatgagcct
      241 acaaacccga ttaatcaaac ctttggacgt tttaatgtta cgtggcaacc aaagttttgg
      301 agaggctggc gaacatggta aaagtagtat gccacctagt ccttctgttt tatcaggtgc
      361 attgcgttct ttttggttgg ctcagtccaa catagatatg caaaagttcg ccaaaatgtc
      421 accaagtgac ttaaaagaac cactgtgttc acaacttggt acacccagcc aaacaggtag
      481 ttttagactt tcagattgtg gtgtagtccg taaaaatagc aataactatg aacggttata
      541 tcctctccca tcagatttag tcttgcaaaa attgcctaaa gataatgatt cattaacacc
      601 ctatgcttta caaccacaaa cactacataa tgacttaatg ggttgccata atggccaact
      661 ggcgatatta aaagctccag ctggtaagcc agaaggcggc tattggctca ctgaatcagg
      721 gtttaatagc tacttaaaag gccaattacc aacaataaaa gacttaatca aaaccagcga
      781 attgtggaaa tcagattggc gtttaggtat tgcattaaat agcgaatcgc gcacagcaag
      841 cgaaggtcaa ctgtacacaa ccgaggcgat tgccttacaa aaagacgttt ttttgatggt
      901 atctattgca ggcgcgaatg actttcccaa agaaggaact ttacgtttgg gcggtgacgg
      961 acgcgccgca ggttttgaat cggtagcttt atcacagtta gacaccattc aagccac
//
LOCUS       DAOQMB010000913         1017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146662_length_1017_cov_2.770270, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000913 DAOQMB010000000
VERSION     DAOQMB010000913.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146662_length_1017_cov_2.770270"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..>1017
                     /locus_tag="PLY05_14945"
     CDS             108..>1017
                     /locus_tag="PLY05_14945"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNC04637.1"
                     /translation="MFSLLAACGGGGGDTEPQQTTNQLQQSTNNPSNTATTPNISCRL
                     GQINMNGVCRSAENVCTADAEKIWVRGFLDDTYLWYDEIKDVKPADYTTSRHYFDALL
                     VKDKDRFSYTMSKEEAEGFFESGLQLSYGILWAYDQDNYLRVRFVEPNSAAAQAGVMR
                     GDYVASLNNQEINTLSFEQIEQLLKPQPLVQLSIKFLDVGKGYYKTAVLTGQNLVTSP
                     VPYYHVLTNNNNVQDKVGYLLFNEHIATASDPLVEAISYFKQQQISDLVLDLRFNSGG
                     YMYVANELAAMIGGKKTANKLFQRYVF"
BASE COUNT          324 a          155 c          215 g          323 t
ORIGIN      
        1 gatgtttatt taagagagtt agataattta taggatataa tagctaagat tttagtttag
       61 agtattaacg aatgtatgcg tcaatcaaag gattgtgtat ctttattatg ttcagcttgt
      121 tagcggcctg tggtggcggt ggtggagaca ctgaacctca gcaaacgact aatcaattgc
      181 agcaatctac caacaatccc tcaaacacag ctactactcc taatataagt tgtcgtttag
      241 gacaaattaa tatgaatggc gtttgtcgtt cggcagaaaa tgtgtgcacg gcggacgctg
      301 aaaaaatttg ggtacgtgga tttttagatg atacctattt atggtatgac gaaattaaag
      361 atgttaagcc agcagattat acaacgtccc gtcattattt tgatgcgttg ttagtcaaag
      421 acaaagatag atttagttat actatgtcaa aagaagaagc tgagggcttt tttgaatcag
      481 gcttacaatt aagttatggc atattatggg catatgacca agataattat ttacgcgtca
      541 gatttgttga gccaaattct gcggcagctc aagcaggggt catgcgtggt gattatgtgg
      601 ctagcttaaa taatcaagaa ataaatacct tgagttttga gcaaattgag cagttattaa
      661 aaccacagcc gctggttcag ctttctataa aatttttaga tgttggtaaa ggttattata
      721 aaacggcggt attaacagga caaaatttgg tcacttcacc tgtgccttat tatcatgttc
      781 ttacaaataa caacaacgtc caagacaaag tagggtattt attatttaac gaacatattg
      841 cgactgcatc tgacccttta gtagaggcaa tcagctattt taagcaacaa caaattagcg
      901 atttggtttt agatttacgt tttaatagtg gcggttatat gtatgtcgct aatgaactag
      961 ccgcaatgat tggtggtaaa aaaacggcca ataagttgtt tcagcgttat gtattca
//
LOCUS       DAOQMB010000914         1016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_146825_length_1016_cov_15.894901, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000914 DAOQMB010000000
VERSION     DAOQMB010000914.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_146825_length_1016_cov_15.894901"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="PLY05_14950"
     CDS             complement(<1..977)
                     /locus_tag="PLY05_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004950820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNC04638.1"
                     /translation="MSQEFDVVVIGGGPGGYEAAIRAAQLGLKVACIEKRIHNGKPSL
                     GGTCLNVGCIPSKALLDSSHRYHDAKEGLAVHGITVGDVSIDIAQMQSRKDAVVSQLT
                     GGVAQLLKGNGIVWLQGTGTLLANKQVQFVAHDGATDTIQATKAVILAAGSAPINIPV
                     APLTEDIIVDSTGALAFSEVPKRLGVIGAGVIGLELGSVWSRLGSEVVVLEALDSFLP
                     MLDKLVSKEAQKILTKQGLEIRLGAKVTGSQINNGVVTVTYQDKEGEKTAEFDKLIVA
                     VGRKPYAQALLDTSSGVSQDERGFVIVDKQCRTNVEGVYAIGDLVRGPM"
BASE COUNT          312 a          256 c          162 g          286 t
ORIGIN      
        1 aacattgggc cacggactaa gtcaccaatc gcataaacgc cttctacatt agtacggcat
       61 tgtttatcta cgatgacaaa accacgctca tcttggctaa caccactgct cgtatctaat
      121 aaggcttgag catacggctt acgaccaaca gctacaatta acttatcaaa ctcagcagtt
      181 ttctcacctt ctttgtcttg ataagtcact gtgacaacac cgttgttaat ttgtgagcca
      241 gtgactttag cacctaaacg aatctctaaa ccttgtttag ttaagatttt ttgtgcttct
      301 ttagatacta atttatctag cattggcaag aatgagtcta aggcttctaa aacaaccact
      361 tcagaaccta aacgactcca tacagagcct aactctaaac caataacccc tgcaccaatc
      421 acgcctaaac gcttaggtac ttcactaaaa gctaatgcgc ctgtcgagtc aacaataatg
      481 tcttctgtta acggagcaac tggaatatta atcggtgctg aacctgccgc caaaatcact
      541 gctttagtgg cttgaatggt gtcggttgcg ccatcatgag ccacaaactg tacttgtttg
      601 tttgctaata atgtacctgt accttgtagc cacacaatgc cattgccttt taataattgg
      661 gcaacaccac ccgttaattg gctaaccaca gcatctttac gactttgcat ttgggcaata
      721 tcaatgctga catcaccaac agtaatgcca tgcaccgcta aaccttcttt agcatcatga
      781 taacgatgag aagaatctaa taaggcttta gaaggaatac aacctacgtt taagcatgta
      841 ccacctaaac ttggtttgcc attatgaata cgcttttcga tacaagcgac ttttaaacct
      901 aattgagcag cacgaatagc ggcttcataa ccacctggcc caccaccaat gacaacaaca
      961 tcaaattctt gggacatctt taaaatcctc aataacattc atattccctc ccctaa
//
LOCUS       DAOQMB010000915         1015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_147130_length_1015_cov_17.886458, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000915 DAOQMB010000000
VERSION     DAOQMB010000915.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147130_length_1015_cov_17.886458"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="hflK"
                     /locus_tag="PLY05_14955"
     CDS             complement(<1..908)
                     /gene="hflK"
                     /locus_tag="PLY05_14955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HNC04639.1"
                     /translation="MAWNQPNGGNNNGGSNGQRPPEIDDFLKKINQNFDKWFGGGGQQ
                     GNSDTTPFKIGLAIVGTLTILSGFYRVEQAEEAVVLRFGKYNSTESAGLHWAMPLVDE
                     VKKINIQKVEQVPLTARMITEDTNIVEINLSVQYRIAQPVDYLLKVSQPELSLLHATE
                     SALRHVVGGSKMGQVLNEGRATLARDIKPRLQGYLNQYKTGLEVTGVNIIEALPPKEV
                     KAAFDDVIRAKEDKERLKNQAETYANGIVPEARGQAARLLAEANAYKLEVVDRAKGDA
                     ARFDQLVVEYKKSPQVIRQRLYLETM"
BASE COUNT          270 a          232 c          184 g          329 t
ORIGIN      
        1 tccatggttt ctaaatacaa acgctgtcta atgacttgtg gcgatttttt gtattcaacc
       61 actaattgat caaaacgtgc ggcatcacct ttggctcgat caaccacttc tagtttatag
      121 gcgttagctt ccgccaataa acgcgccgcc tgaccacgtg cttctggcac aatcccatta
      181 gcataggttt cggcttggtt ttttaggcgt tctttgtctt ctttggcacg aattacatcg
      241 tcaaaagccg ctttaacctc tttggggggt aacgcttcaa tgatgttaac gccagtcact
      301 tctaaaccag ttttatattg gtttaagtag ccttgtaagc gtggttttat gtctctggct
      361 aaggtagcgc ggccttcatt gagcacttga cccatttttg aaccgccaac aacgtgtctt
      421 aatgcgcttt cggtggcgtg taataaactt aattcgggtt gagagacttt taataaataa
      481 tcaacaggtt gggcaattcg atattgcaca cttaaattga tttcaacaat attggtatct
      541 tcggtaatca tgcgtgcggt taatggaact tgctcaacct tttgaatatt aatctttttt
      601 acttcatcaa ctaaaggcat tgcccaatgt aaacctgcac tttcggtact attgtattta
      661 ccaaagcgca aaacaaccgc ttcttcggct tgttcaacac gataaaaacc acttaaaatg
      721 gttaatgtac caacaattgc taaaccaatt ttgaatggcg ttgtatcaga gttaccttgc
      781 tggcctccac caccaaacca tttatcaaaa ttttggttga ttttcttcaa aaaatcatca
      841 atttctggcg gtcgttggcc attagagcca ccattgttgt taccaccatt cggctgattc
      901 cacgccatgc tttacctcat tattaggact gtggttttta ccactcagga acaatttcag
      961 ggtcagaaag tcctacatca ctacgtttta aacccatacg ccgcaacaaa atatc
//
LOCUS       DAOQMB010000916         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_147435_length_1014_cov_16.976017, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000916 DAOQMB010000000
VERSION     DAOQMB010000916.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147435_length_1014_cov_16.976017"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1014
                     /locus_tag="PLY05_14960"
     CDS             <1..>1014
                     /locus_tag="PLY05_14960"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HNC04640.1"
                     /translation="KLSTLAGYGTAWIAPNPISAFLMSQGNVTRWTTIAHHVLHKGFD
                     KIEGIPERYTSKGFAKGWRRFLDWNDWMLPEAWSNEHNKLHHYNLGEAADPDQVELNL
                     EWLRDSKLPMPLRYAVVALFAATWKPLYYAPNTLKHLTSTKNNVLGEERDSATLTQLA
                     LWNPLNPAARDLWSKCLLPYIFVKFVLIPLAFTPLGPIAVINVWINTLLAEIISNLHA
                     FLVIVPNHVGNDILLFEDVANNKSEFFLRQITGSTNFKTGGDLNDMLHGWLNYQIEHH
                     LWPDLPMSEYQRLQPQVKALCEKYGIPYVQESVWVRLKKTVDVMVGNSSMPRQKSVSS
                     NKRS"
BASE COUNT          296 a          199 c          212 g          307 t
ORIGIN      
        1 aagctgtcaa cattggcagg ttatggcact gcatggatcg cacctaatcc catttctgca
       61 tttttaatga gtcaaggcaa cgtcacacgt tggacaacca ttgctcatca tgtattacac
      121 aaaggctttg ataaaattga aggcattcca gagcgttata ccagtaaagg ttttgccaaa
      181 ggctggcgtc gctttttaga ttggaatgat tggatgttgc ccgaagcatg gagtaacgaa
      241 cacaacaaac tccatcatta taacttaggc gaagcagctg accctgatca ggtagaatta
      301 aacttagaat ggttacgtga ctctaaactg cctatgccat tgcgttatgc ggttgttgcc
      361 ttatttgcgg caacatggaa gccgttatac tacgctccaa ataccttaaa gcacttaaca
      421 agcaccaaaa ataatgtttt aggtgaagag cgtgactccg ctaccctaac ccaattagca
      481 ttatggaatc ccctaaatcc tgctgctcgt gatttgtggt cgaagtgttt gttgccttat
      541 atctttgtta aatttgtttt aattccctta gcatttacac cattaggccc aattgcggta
      601 attaatgtct ggattaatac gttattggcc gaaattatta gcaacctcca tgctttttta
      661 gtgattgtgc caaaccatgt gggtaatgat attttgttat ttgaagatgt tgccaataac
      721 aaaagtgagt tcttcttacg ccaaattact ggctcgacca actttaaaac aggtggagac
      781 ttaaatgata tgttacatgg ctggttaaac taccaaattg aacatcattt atggcctgat
      841 ttaccaatga gcgaatacca acgtttgcag ccacaagtca aagctttgtg tgaaaaatat
      901 ggtattcctt atgtgcaaga atcggtgtgg gtgcgtttaa agaaaaccgt tgatgtgatg
      961 gttggcaata gctctatgcc gcgtcaaaaa tcggtgagta gcaataagcg gtct
//
LOCUS       DAOQMB010000917         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_147436_length_1014_cov_15.654849, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000917 DAOQMB010000000
VERSION     DAOQMB010000917.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147436_length_1014_cov_15.654849"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..487)
                     /locus_tag="PLY05_14965"
     CDS             complement(71..487)
                     /locus_tag="PLY05_14965"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-responsive transcriptional
                     regulator"
                     /protein_id="HNC04641.1"
                     /translation="MQLKIGELAQKTHVSTATLRYYEQCGLLSRANRADNGYRYYPVE
                     AIQQIEFIKKAQQLGFSLQDIATILQIRQTGQAPCQVVKHLLTEKIAQVQNQLETLKH
                     LEAELLYYQQQWQQEANVSNATGSICHLIEEISIKN"
     gene            595..717
                     /locus_tag="PLY05_14970"
     CDS             595..717
                     /locus_tag="PLY05_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04642.1"
                     /translation="MNKMLMLLAMLGMFSTSVYAADCCEDHQPCCEHQHPCCDE"
     gene            777..>1014
                     /locus_tag="PLY05_14975"
     CDS             777..>1014
                     /locus_tag="PLY05_14975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HNC04643.1"
                     /translation="MFALNRRQFITGCSTGIAVASMGISRHALAKTQPRQAHYQPILR
                     GNQFKLTVGRQDINFTGKTRQAVTVNGSTPAPTLY"
BASE COUNT          289 a          211 c          189 g          325 t
ORIGIN      
        1 aatcttacca atatcactca aacccaaagc atcggcagct aaacttttaa acattcttga
       61 ctccatgtag ttaatttttt atgctgattt cttcaatcaa atggcaaata gaaccagtag
      121 catttgatac attggcttct tgttgccatt gctgttgata atataacaat tctgcttcta
      181 agtgttttaa ggtctctaat tgattttgta cttgtgctat tttttcggtc aacagatgtt
      241 tgacaacttg gcaaggtgct tgacctgttt gtctaatttg taaaatagtc gcaatatcct
      301 gcaaagaaaa acctaactgt tgggcttttt taataaattc aatttgttgt atcgcctcta
      361 caggataata acgatagcca ttatccgctc tattggcacg gcttaataaa ccacattgct
      421 cataataacg tagggttgca gtactaacat gagtcttttg tgccagctcg ccaattttta
      481 actgcatgat ctgtattctc tttgtaacat acacagttgt tgaacttgac cttaaagtcg
      541 actttaagga ctacagtaaa caggccgatt atggcgtatt ttggagcgaa tacgatgaat
      601 aaaatgttaa tgttattggc aatgttgggt atgttttcta cttcagtgta tgccgcggat
      661 tgttgtgaag accatcaacc gtgttgtgaa catcaacatc cttgttgtga cgaataattg
      721 ctaacagcgc ttgagtgtta aacactcaag cctgacatta aacggaataa tcacccatgt
      781 ttgctctcaa tcgtcgtcaa tttattacag gctgctctac aggaattgcc gttgcaagta
      841 tgggcatctc tcgtcatgcg ctggccaaaa cgcaaccgcg ccaagctcat tatcaaccga
      901 ttttgcgtgg taaccagttt aaacttaccg ttggccgcca agacatcaat tttacaggaa
      961 aaacacggca agccgtgacc gtcaatggtt caacacctgc accaacgctg tata
//
LOCUS       DAOQMB010000918         1014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_147459_length_1014_cov_4.232534, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000918 DAOQMB010000000
VERSION     DAOQMB010000918.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_147459_length_1014_cov_4.232534"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1014)
                     /locus_tag="PLY05_14980"
     CDS             complement(<1..>1014)
                     /locus_tag="PLY05_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNC04644.1"
                     /translation="IYHYAPYEVTACRKLMGRFGVCEQEVDDLLRRGVFVDLYKIVKS
                     GLLVGTPNYSIKSIERLYGSEHQGEVANGGDSIVAYENYRNKLANGERAQANTILKNI
                     EAYNKDDCDSTQELTAWLRQLQQEKGISYQASIINVEQKELTDEQKAVLALRDRLLTQ
                     AESLKQDNAIFANQLANIAWMLEFHRREDKPKWWKYFDRMDATEEELWDDLDCLAMCQ
                     RTEREPIPIDDNNVAYEYNFDVNQDFKGGKEEFIVLGLTTDNGKDVKVKLLKEESSLK
                     KGVITLKSSQVLPQTITLIPNDYINPKPIPTALQQVIEAINTQGNTTHCAIQHFLAKQ
                     PPKI"
BASE COUNT          292 a          216 c          156 g          350 t
ORIGIN      
        1 aatttttggc ggttgtttgg ctaaaaagtg ttgtatagca caatgtgtgg tgttgccctg
       61 agtgttaatc gcttcaataa cttgttgtaa tgccgttgga ataggctttg ggttaatata
      121 atcattagga attaaagtta tagtttgcgg taaaacctga ctacttttta gtgtaatcac
      181 gccttttttt agcgaggatt cttcctttaa caatttaact ttaacatctt taccattgtc
      241 tgtggttaat cctaatacaa taaactcctc tttgcccccc ttaaaatctt gattaacatc
      301 aaaattatat tcataagcaa cattattatc atcaataggt ataggctctc tttcagtacg
      361 ttgacacatc gccaaacagt ccaaatcatc ccataactct tcttcggttg cgtccatgcg
      421 gtcaaaatat ttccaccatt taggtttgtc ttcacgtcga tgaaactcta acatccatgc
      481 tatatttgct aattgattag caaatatagc attatcttgt ttgagtgatt cagcttgtgt
      541 taacaaacga tcacgtaagg ctaagacagc tttttgttca tcggtcagct ctttttgttc
      601 tacattaata atggatgctt gatagctaat ccccttttct tgttgcaact ggcgtaacca
      661 agccgttaat tcttgcgtag agtcacaatc atctttatta taggcctcaa tattttttaa
      721 gatagtattg gcttgtgctc tctcaccgtt agccaactta tttctataat tttcataagc
      781 gacaattgag tcaccaccat tagcaacttc cccttgatgc tctgagccat ataaacgctc
      841 tatactttta atcgaataat tgggtgtgcc tactaacaag ccacttttga caattttata
      901 taaatcaaca aacacgcctc gacgcagcaa atcatccact tcttgctcac aaaccccaaa
      961 acgccccatt aacttacgac aagcagtaac ttcataaggc gcataatgat aaat
//
LOCUS       DAOQMB010000919         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_148018_length_1012_cov_7.535005, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000919 DAOQMB010000000
VERSION     DAOQMB010000919.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148018_length_1012_cov_7.535005"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..>1012)
                     /locus_tag="PLY05_14985"
     CDS             complement(32..>1012)
                     /locus_tag="PLY05_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016651160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HNC04645.1"
                     /translation="RFYLSLEDDLMRIFASDRVRNMMRSLGMQKGEAIEHRWVTRSIE
                     GAQRKVEARNFDIRKNLLKYDDVANEQRRVIYHQRDEVLASESLATMVEDMYEEVVGT
                     VVTEFIPPQSIEDQWDIAGLEKALAADFKMDVPVKAWLDADDRLEENGLRAKIGQAAR
                     DAYRQTRIKMGDDIANMIEHQIMLQVLDRHWKEHLAAMDYLRQGIHLRGYAQKNPEQE
                     YKREAFQLFQQLLGAVKLEVVQTIATIHIPSEEEVAAAQAEQAAQMAAMEMHMEHPAL
                     LSSEEGMPAVVDESTPDNGENPYADQYVGRNDPCPCGSGKKYKQCHGRLA"
BASE COUNT          285 a          254 c          171 g          302 t
ORIGIN      
        1 ggtctgatct catttaattt tcacagagag tctaagctaa acgcccatga cattgtttgt
       61 attttttgcc actaccacaa ggacacggat cattacgacc aacatattgg tctgcataag
      121 gattctcacc attgtctggt gtagactcat caacaaccgc aggcatacct tcttcgctac
      181 ttaataaagc aggatgctcc atgtgcattt ccatcgcagc catttgtgca gcttgttcag
      241 cttgtgccgc agcaacttct tcctcagaag gaatatgaat cgtggcaatg gtttgcacca
      301 cttctaattt aacagcaccc aataattgtt ggaacaattg gaaagcttcg cgtttgtatt
      361 cttgctctgg attcttttgg gcgtaaccac gtaaatgaat cccctgacgc aaataatcca
      421 ttgctgctaa atgctctttc cagtgacgat ctaatacttg caacatgatt tgatgttcaa
      481 tcatattagc aatgtcatca cccattttga tacgggtttg acgataagcg tcacgtgctg
      541 cctgaccaat tttggcgcgt aaaccatttt cttccaaacg atcgtcagca tccaaccaag
      601 ccttaactgg cacatccatt ttaaaatcag ccgctaaagc tttttctaaa cctgcaatat
      661 cccattgatc ttcaatactt tgtggtggaa taaactctgt tactacagta ccaaccactt
      721 cttcatacat atcttctacc atcgtcgcta aactttcaga ggctaaaacc tcatcacgct
      781 gatggtaaat aacacgacgc tgctcgttgg caacgtcatc atatttaagc aagtttttac
      841 gaatatcaaa gttgcgtgct tcaaccttac gttgcgcgcc ttcaatcgaa cgtgttaccc
      901 aacgatgttc aattgcctca cctttttgca tacccaatga acgcatcatg ttacgcacac
      961 gatcactggc aaaaatacgc atcaaatcgt cttctaaaga caagtaaaaa cg
//
LOCUS       DAOQMB010000920         1012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_148072_length_1012_cov_3.388715, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000920 DAOQMB010000000
VERSION     DAOQMB010000920.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148072_length_1012_cov_3.388715"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="PLY05_14990"
     CDS             <1..828
                     /locus_tag="PLY05_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HNC04646.1"
                     /translation="NITVIHFHDLVRLACEQSGTPYDVPEDKEALSLFFQEGCADLLL
                     HAVEQGAKKYDTIIVDEAADFRTTWWIAIEALGLPAFSWYCFYDKHQAIFWEHEPWHA
                     PFAAQVMPLEVNLRNTQPVGTLAANLGHCPLPADFRIKSGIKPELCVCDDFDVMATKL
                     QQLLHHLINEEQVSPQRIAILSPYRYTNLKSTWSNGLKTFVVNEEVATPIKGQIAIAT
                     IQSFKGLESDIVILVGITKQSANNKELLYVGASRAKVGLYILSLVELDQQKKSVRHE"
BASE COUNT          310 a          165 c          218 g          319 t
ORIGIN      
        1 aatatcaccg taatacattt tcatgatttg gtgcgtttag cctgtgagca atcaggtacg
       61 ccttatgatg tacctgaaga taaagaggca ttatcattgt tttttcaaga gggttgtgca
      121 gatttattac ttcatgcagt tgagcaggga gctaaaaaat acgatacgat aattgttgat
      181 gaggcagcag attttagaac aacttggtgg atagcaatag aagcattagg attaccagca
      241 tttagttggt attgctttta tgataagcat caagctattt tttgggagca tgagccttgg
      301 catgcaccgt ttgccgctca agtgatgccc ttagaagtta acttacgtaa tacgcaacct
      361 gttggcacct tagcagctaa tttgggacat tgccccttac cagcagattt tagaataaag
      421 tcagggataa aacctgaatt atgtgtttgt gatgattttg atgtaatggc aacaaagcta
      481 cagcaattgt tgcatcattt aatcaatgag gagcaagtct cacctcaaag aatagctatt
      541 ttaagtccgt atcgttatac caatctaaaa tcaacgtggt caaatgggct aaagactttt
      601 gtagttaatg aagaagttgc cacaccgata aaaggtcaaa tagcgattgc cactattcaa
      661 agttttaaag gattagagtc tgatattgtc attttagtcg gtattactaa acaaagcgca
      721 aacaataaag agttgttgta tgttggcgca agtcgcgcaa aagtgggctt atatattcta
      781 tcgttagttg agctagacca acaaaaaaaa tcagtgaggc atgaatgaaa tatgcctcat
      841 attgcgttat ttatgtttgg actctaatag ctcagtttta tgtgtagcta aagaggtttg
      901 aggctttgtt tctagtttag ccatctgtcc tgaaatgagc tgattaacat ggtcatggat
      961 tttatctttg gctgttaaaa ccgaattgac aacggcagta cgcctttcac gt
//
LOCUS       DAOQMB010000921         1011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_148451_length_1011_cov_2.711297, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000921 DAOQMB010000000
VERSION     DAOQMB010000921.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_148451_length_1011_cov_2.711297"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="PLY05_14995"
     CDS             <1..258
                     /locus_tag="PLY05_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04647.1"
                     /translation="NQDPHKPEAVIQEIAQKIKAGRTAYQKALAKKVNATEVVQSVKQ
                     AGLKNTQKMRVASLEEQLKAIENVIVERTAYKTQLEAELAA"
     gene            258..>1011
                     /locus_tag="PLY05_15000"
     CDS             258..>1011
                     /locus_tag="PLY05_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04648.1"
                     /translation="MIEPLSSKWGGDNYGINQHLIAKLYAVDGNGKADDPNLAVAAPL
                     TDGNLDMSFNWQSPFENSGTDSKLPAISQLLQSGQASIMLNLMSKFFKDDDPNSNISK
                     FNNAVAETAKKAAELTGRTGITKLNSRQVFSGMPPIKIPITMYFRAWSDPLNEVETPV
                     NQLFEWALPQNLADNSILADALQMLLDGKVPDLNTLFPSVIPRFVALEYGGRTFKPLV
                     IESIAQPLVLPRSKDGEMLSVSVQLTLATLTAW"
BASE COUNT          338 a          202 c          214 g          257 t
ORIGIN      
        1 aaccaagacc cgcacaaacc cgaagctgtt atccaagaaa ttgctcaaaa aatcaaagca
       61 gggcgcacag cctatcaaaa agccttagct aaaaaagtta atgctacaga ggtagtgcag
      121 tctgtgaaac aagcagggct taaaaacact caaaaaatgc gagttgcctc actagaggaa
      181 cagttaaaag cgattgagaa tgttattgtg gagcgtacag cgtacaaaac acaattagag
      241 gcggagttgg cagcataatg atagaaccac tatcgagcaa atggggcggc gataattacg
      301 gcattaatca acatttaatt gcaaaattat atgccgttga tggaaacggc aaggccgatg
      361 accctaactt agccgttgcc gcaccattga ccgatggtaa cttagatatg agttttaatt
      421 ggcaaagtcc ttttgaaaac tcaggcacag attcaaaact accagcgatt agccaattat
      481 tacaaagtgg ccaagcgagt attatgttga atttaatgtc taaatttttt aaagacgatg
      541 accctaatag taatatctct aagtttaata atgctgtagc cgaaacagcc aaaaaagccg
      601 cagaattaac aggacgaaca ggcattacca agctcaatag ccgccaagta tttagtggga
      661 tgccgcctat taaaatcccg attacgatgt attttagggc atggagtgac cctttaaatg
      721 aagtggaaac acctgttaat cagttgtttg agtgggcatt accacaaaac ttagccgata
      781 acagcatttt agccgatgct ttacaaatgt tgttagacgg taaagtcccc gatttaaata
      841 ccttattccc ctccgtgata ccgcgttttg tcgctttaga atatggtgga cgaaccttta
      901 aaccattggt gatagaaagc attgcacagc ctttagtttt gccgcgttct aaagacggtg
      961 agatgttaag cgtatccgtg caattaactt tagccacttt aacagcatgg g
//
LOCUS       DAOQMB010000922         1008 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_149190_length_1008_cov_13.895068, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000922 DAOQMB010000000
VERSION     DAOQMB010000922.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149190_length_1008_cov_13.895068"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLY05_15005"
     CDS             complement(<1..338)
                     /locus_tag="PLY05_15005"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNC04649.1"
                     /translation="MSESPLRIAINGYGRIGRHVLRAWVERDCPASLRFVAINDLGQA
                     QALMHLTRYDSTHGRFNGHADLVDGQLAIRPTDGQQQSLIPLFNQAKPELLPWQELDV
                     DVVLECTGQF"
     gene            complement(372..806)
                     /locus_tag="PLY05_15010"
     CDS             complement(372..806)
                     /locus_tag="PLY05_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HNC04650.1"
                     /translation="MNSSKYQEDFYAWTQEQAQLLRSRQLAKIDIANLIDEIESMGKS
                     EKRALKSRLSILLMHLLKWQFQPNFRGTSWQLTLRTQRRAIKDLLADNPSLKHWLIET
                     MHDAYEQAQDDASLETGLNSSIFPNQCPWSIEQILDQDYLPN"
     gene            complement(875..>1008)
                     /locus_tag="PLY05_15015"
     CDS             complement(875..>1008)
                     /locus_tag="PLY05_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003659212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04651.1"
                     /translation="YKFVGLDGKVVGMTSFGESAPIKDLYQHFGITVEAVVEAVNSL"
BASE COUNT          295 a          238 c          172 g          303 t
ORIGIN      
        1 ctaaattgtc ccgtacactc taataccaca tctacatcaa gctcttgcca cggcaataac
       61 tcgggcttcg cttggttaaa caaaggaatt aagctttgtt gctgaccatc tgtgggacga
      121 attgctaatt gcccatcaac taaatcggca tgaccgttaa aacggccatg tgtactgtca
      181 taacgcgtaa gatgcattaa ggcttgagct tgtcctaaat cattaatcgc gacaaaacgt
      241 aaggatgcag gacaatcacg ctctacccaa gcgcgtaaaa cgtgtcgtcc aatacgtcca
      301 tagccattaa tggcaatacg aagcggtgat tcactcatca aacgttcatt ttcctaagct
      361 cttcatttga ttcaatttgg gaggtagtct tggtccaaaa tttgctctat cgaccacgga
      421 cattgattag gaaaaatact actatttaat cctgtttcca aagaggcatc atcttgagct
      481 tgttcataag catcatgcat cgtttcaatt aaccaatgtt ttagactggg attatccgct
      541 aataagtctt taatcgcacg gcgttgagtt cgcaaggtca attgccagct tgtccctcta
      601 aaattaggtt gaaactgcca ttttaataag tgcatcagta aaatacttag gcgagacttg
      661 agtgcgcgct tttcgctttt acccatgctt tcaatctcat caattaaatt agcaatatca
      721 attttggcca attgacgact acgcaacaat tgcgcttgct cctgtgtcca cgcataaaaa
      781 tcttcttggt atttgctact attcatcgtt tcacctctac cattcatgca gtcaacataa
      841 gcataacgta tttgctgtgt ttatggatta ccactcacaa gctattcacc gcttcaacta
      901 ccgcttctac ggtaatacca aaatgttgat ataaatcttt aataggtgca gactcaccaa
      961 agctggtcat accaaccact ttgccatcta aaccaacaaa tttatacc
//
LOCUS       DAOQMB010000923         1006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_149844_length_1006_cov_3.794953, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000923 DAOQMB010000000
VERSION     DAOQMB010000923.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_149844_length_1006_cov_3.794953"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLY05_15020"
     CDS             complement(<1..352)
                     /locus_tag="PLY05_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04652.1"
                     /translation="MTEVKKTVIITGAAGGMGRETCRYLALQGWKVLAIDHNQVRLAE
                     LAQSHVHIKPLFADLTDITLVSQVQHQLQDMPPVMGLVNLAGASIGNTIDLLEEDDWQ
                     QSFAINVSPAMRLIQ"
     gene            complement(370..699)
                     /gene="csx16"
                     /locus_tag="PLY05_15025"
     CDS             complement(370..699)
                     /gene="csx16"
                     /locus_tag="PLY05_15025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx16"
                     /protein_id="HNC04653.1"
                     /translation="MAKLWIVSRHQGAIAWLHAQGWQGTQIDHLDIDCVSEGDVVMGT
                     LPVNLAAEVCAKKAQYIHLSLRIPFALRGVELTSEQMMYYGADLQAYDISQTKIDIQA
                     LKSYLAN"
     gene            complement(710..>1006)
                     /locus_tag="PLY05_15030"
     CDS             complement(710..>1006)
                     /locus_tag="PLY05_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04654.1"
                     /translation="HQAALINAQQAQQHFKHLLFEEAWATKLVCALTGRAWQTDNLQQ
                     LILNITPIQPPSFEIVSDKIWGDTHINDNNGFASLLNKQVDIDAVYDCWTLFFI"
BASE COUNT          274 a          226 c          195 g          311 t
ORIGIN      
        1 cctgaattaa acgcatggca ggtgacacat taatggcaaa agactgttgc caatcgtctt
       61 cttctaataa atcaatggta ttgccaatag acgcgcccgc caaattcact aagcccatca
      121 ctggcggcat gtcttgtaat tgatgttgta cttggctaac taatgttata tcggttaagt
      181 cggcgaaaag tggcttgatg tgtacgtgtg actgagcaag ttcggctaat cgcacctgat
      241 tgtgatctat agccaatacc ttccacccct gcaatgccaa ataacgacag gtttcccgtc
      301 ccatgccgcc tgctgcacct gttataatca cggttttttt gacttcagtc atgcctactt
      361 ctccttttat tagttagcta aatagctttt tagtgcctga atatctatct tggtttggct
      421 aatatcgtat gcttgtaaat ctgcgccata gtacatcatt tgttcgctag ttaattcaac
      481 accacgcaaa gcaaagggaa tacgcaaact caaatgaata tattgtgctt ttttggcaca
      541 gacttcggcg gctaaattga cgggtaaagt tcccatcacc acatcaccct cagacacaca
      601 atcaatatct aagtggtcaa tttgtgtacc ttgccaacct tgcgcatgta accaagcaat
      661 cgctccttga tgacgagaaa caatccataa tttagccatg atttgccccc taaataaaaa
      721 acaacgtcca gcaatcataa actgcatcaa tatcaacttg cttatttaac aagcttgcaa
      781 agccgttgtt gtcattgatg tgtgtgtcgc cccatatttt atcactgacg atctcaaatg
      841 acggcggctg gattggtgtg atatttaaga ttaattgttg aaggttatcg gtctgccatg
      901 cacgccccgt taatgcacaa actaattttg ttgcccatgc ttcttcaaag agtaaatgtt
      961 tgaagtgttg ttgtgcttgt tgagcattta ttaaggctgc ttggtg
//
LOCUS       DAOQMB010000924         1005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_150119_length_1005_cov_18.341053, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000924 DAOQMB010000000
VERSION     DAOQMB010000924.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150119_length_1005_cov_18.341053"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..354)
                     /locus_tag="PLY05_15035"
     CDS             complement(64..354)
                     /locus_tag="PLY05_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNC04655.1"
                     /translation="MEQAQQILITKLIQREAVACRQFIQEHHSRLVSIARTIVGDGAE
                     DVVQEAWLQAFKALPNFEGRASLKTWLTRITVNAAYGRCRYEKSRVALSNSP"
     gene            553..1005
                     /locus_tag="PLY05_15040"
     CDS             553..1005
                     /locus_tag="PLY05_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04656.1"
                     /translation="MRKFCYLVALMFASTTFAADISLTMHAEKEVPVTIQGSVVPMLK
                     PVRKVNVGDTLVITLRYKNNENQEAYNVNIDNPIPVGTRFISGSGAGKGAVFYVSYDN
                     GESYEEDIRVHNKPVTHVRWSFENLAANAEGEVSFNLKIEKADHQLMR"
BASE COUNT          316 a          201 c          183 g          305 t
ORIGIN      
        1 agccgaagta ttgataagct gtcgtgtcac cgccttatat cctcggccta aaggcgcgag
       61 gttttacggc gagtttgata aagcaacacg gctcttttca taacgacaac gaccataagc
      121 tgcattgaca gtaatacgtg ttagccatgt ttttaagcta gctctaccct caaaatttgg
      181 caacgcctta aatgcttgca accatgcttc ttgtacaaca tcttcagcac catcacccac
      241 aatcgtccgc gcaatactta ccaaacgact atgatgctct tgaataaatt gtcgacaagc
      301 aacagcctct cgctgaatca gctttgtaat taatatctgt tgagcttgtt ccatttgtta
      361 ttaaaactct aaaaatatca ttgatgagga tataacaaga aatagctact cagccagttt
      421 aaatcgaata aatttaccta cagctccaag tcattactat ttagatattt ttaagacaag
      481 tttgttacat ggcctaccca ataaagatag attacggcat gattagatgg tgatatttta
      541 aggacgtgga ttatgcgtaa attctgttat ttagtcgctt taatgtttgc atctaccaca
      601 tttgctgcag atatttcctt gacaatgcat gcagaaaaag aagtaccagt tactattcaa
      661 ggttctgttg taccaatgct taaacctgtc cgtaaagtca atgttggtga cacgctagtt
      721 attactttac gttataaaaa caacgaaaat caagaagctt ataacgtcaa tattgataat
      781 cccattcctg tgggtacacg ttttatatcg ggttcaggag cgggtaaagg tgccgttttt
      841 tatgtttctt atgataatgg cgaatcctat gaagaagata tccgtgtaca taacaaacct
      901 gtcacccatg tacgctggag ctttgaaaat ttagcggcta atgccgaagg agaagtcagc
      961 tttaatctaa aaatagaaaa agctgaccac caattaatgc gttaa
//
LOCUS       DAOQMB010000925         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_150427_length_1004_cov_18.761855, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000925 DAOQMB010000000
VERSION     DAOQMB010000925.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150427_length_1004_cov_18.761855"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1004
                     /locus_tag="PLY05_15045"
     CDS             <1..>1004
                     /locus_tag="PLY05_15045"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNC04657.1"
                     /translation="MQRRQALQKIAALGAATATTASFAAPAVITQASVQWRLASSFPK
                     SLDTIYGAGEMLSKRVAQLTDGKFQIRVFAGGELVPGLQVLDAVSNGTVECGHTAGYY
                     YVGKSKAFAFDTCLPFGLTARQQNAWYSQAGGQQLLGELFKQYNIVNFAGGNTGAQMG
                     GWFRKPVNSLKELQGIKMRIPGIGGEIMSRLGVIPQTIAGADIYPALERGAIDASEWV
                     GPYDDEKLGLYKVAKHYYFPGWWEAGPQLSFYVNQSAWAKLPKAWQSAFEVATMEANQ
                     MMLAAYDARNPQALIRLMNQHGVVLHRYPDDVMQAGQKAAFALYDELSAADPMFKKIY
                     "
BASE COUNT          270 a          207 c          252 g          275 t
ORIGIN      
        1 catgcaacgt cgtcaagcct tacaaaaaat tgctgcttta ggcgcagcca ctgcgacaac
       61 cgccagtttt gccgccccag cagttatcac acaagctagc gtacaatggc gtttagcctc
      121 tagttttcct aaaagtttag acaccattta tggtgcaggc gaaatgctct ctaaacgtgt
      181 tgcacagtta actgatggta aatttcaaat tcgcgtgttt gcaggcggag aattagttcc
      241 gggtttacaa gtcttagatg ccgtgagtaa tggtacggtc gaatgtggcc acaccgcagg
      301 ttattactat gtgggcaaaa gcaaagcctt tgcctttgat acctgtttac cttttggttt
      361 aaccgctcgt caacaaaatg catggtattc acaagcaggc ggtcaacaat tattgggcga
      421 gttatttaag caatataaca tcgtcaattt tgcaggtggc aatacaggcg cgcaaatggg
      481 cggttggttt agaaaacccg tcaatagcct taaagaatta caaggcatca aaatgcgtat
      541 tccagggatt ggtggcgaaa tcatgtcccg tttgggcgtg attccccaaa ccattgcagg
      601 ggcagatatt tatcctgcct tggagcgtgg tgcaattgat gccagtgaat gggttgggcc
      661 ttacgatgat gaaaagttag gcttatataa agtcgccaaa cattattact ttccgggttg
      721 gtgggaagca gggccacagt taagttttta tgtcaatcag agtgcatggg caaaattgcc
      781 taaagcatgg caatcggcgt ttgaagtggc cacgatggag gctaatcaaa tgatgttagc
      841 cgcttatgat gcgcgtaatc cgcaagccct cattcggtta atgaatcaac atggggtggt
      901 gttgcatcgt taccctgatg atgtcatgca agcagggcaa aaagctgctt ttgcgttata
      961 tgatgagtta tccgccgccg acccgatgtt taaaaagatt tatg
//
LOCUS       DAOQMB010000926         1004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_150429_length_1004_cov_16.979979, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000926 DAOQMB010000000
VERSION     DAOQMB010000926.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150429_length_1004_cov_16.979979"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..667)
                     /locus_tag="PLY05_15050"
     CDS             complement(158..667)
                     /locus_tag="PLY05_15050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNC04658.1"
                     /translation="MSKQFLLKTIQAQDLASIQKIQRAAYPQYFWEDDAVFMAKQQYA
                     PESCLLLHTAEQQAVAYVFAHPWPIGQIPALNQALQLGTDTEQVLYIHDLAVHPEWHG
                     QGLAQHLVKHLVIYAQQENFSQMALVAVEQAQAFWQRLGFITKNYPQLDAKLATYGQY
                     AVYMTKTIT"
     gene            complement(660..779)
                     /locus_tag="PLY05_15055"
     CDS             complement(660..779)
                     /locus_tag="PLY05_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04659.1"
                     /translation="MSQQTQERLGKEFITIERGVIDVKGKGEMTTWWLEAERV"
     gene            complement(776..>1004)
                     /locus_tag="PLY05_15060"
     CDS             complement(776..>1004)
                     /locus_tag="PLY05_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNC04660.1"
                     /translation="IMSELSQANEKDCQIRQYIRRPDKQFWLDLLNPPPLPTQADHTR
                     HTRITRYAIGAAFALTTFYFLISFFMGGAFK"
BASE COUNT          290 a          198 c          206 g          310 t
ORIGIN      
        1 ggattggcaa aactttgcaa aatagacatg gcaacctctc aacaagcacg gctgttcctt
       61 gtgctaggtt ttaaagttgc accttaaggc ttaaaaatga caatttggtg tcatgttatt
      121 gacagtttgt tgacagcttg gcaagttgtg aaaacactca cgttattgtt ttggtcatat
      181 atactgcata ttgaccataa gtcgctaatt tagcatctaa ttgtggataa ttttttgtga
      241 taaaacctaa acgctgccaa aatgcttgag cttgctctac cgctaccaaa gccatttggc
      301 taaaattttc ttgctgtgca tagatgacta aatgtttgac tagatgctga gccagtcctt
      361 gaccatgcca ctcaggatgt acagctaaat catggatata caacacttgt tctgtgtctg
      421 tgcctaactg taaagcttga tttagtgcag gaatttggcc gattggccaa gggtgagcaa
      481 aaacataagc taccgcttgt tgctcggctg tatgcaacaa taaacaactt tcaggggcat
      541 attgttgctt ggccataaac acagcatcat cttcccaaaa atattgagga taggcagctc
      601 gttgaatttt ttgaatactc gctaagtctt gcgcttgaat tgttttaagc aaaaattgtt
      661 tagacacgtt ccgcctcaag ccaccatgta gtcatttcac ctttaccttt aacgtcaatc
      721 acaccacgtt ctatggttat aaattctttg cccaaacgct cttgagtttg ttgactcatt
      781 tgaatgcacc gcccataaaa aaggaaatta aaaaataaaa ggtggttaaa gcaaaagcag
      841 cacctatcgc ataccgcgta attcgagtat gacgggtatg gtcggcttga gtcggtaatg
      901 gaggaggatt cagtaaatct aaccaaaact gtttatcggg tcggcggata tactgacgaa
      961 tttggcaatc tttttcgttt gcttggctta attcggacat gatt
//
LOCUS       DAOQMB010000927         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_150765_length_1003_cov_14.042194, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000927 DAOQMB010000000
VERSION     DAOQMB010000927.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150765_length_1003_cov_14.042194"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="PLY05_15065"
     CDS             complement(<1..840)
                     /locus_tag="PLY05_15065"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNC04661.1"
                     /translation="MNNPKQPLIIRLASKLLNPRGLDKPQKLHDAVNNKLILITGASF
                     GIGEETAKHLAKAGAIVLLVARSADKLQAVADDINKAGGRAFIYPTDLSKPEHAKAVA
                     EQILAEHGHVDIVLNNAGKSIRRSIAMSENRFQDFERTMAINYLGPVQLILALLPSMR
                     ARKSGHIINISTWGVKMPAGGRWAAYQASKGAFDTWLRSVSVEIKQDGIATTSIYPAI
                     VYTRMSAPTPWMHKLPGMTTEDAAHVIERAIIEKPYDISPEWLGIGQVTSVLFPTPVR
                     KYLE"
BASE COUNT          275 a          207 c          209 g          312 t
ORIGIN      
        1 ttctaaatat ttacgcacag gtgtgggaaa taataccgat gtgacttggc caatacccaa
       61 ccattcaggt gaaatatcat aaggtttttc aataatggca cgctcaatga catgggctgc
      121 gtcttcggta gtcatacccg gtaatttgtg catccacggt gtcggtgcgc tcatgcgggt
      181 ataaacaatg gctggataaa tagaggttgt ggcaatacca tcttgtttaa tttcaacaga
      241 cacgctgcgt aaccatgtat caaatgcccc tttagaagct tggtacgctg cccaacggcc
      301 acccgcaggc atttttacgc cccatgttga aatattgatg atatggcctg atttacgtgc
      361 tcgcatcgac ggcaataacg ccaaaattaa ctgtacgggc cctaaataat taatcgccat
      421 ggttcgttca aaatcttgaa agcgattttc tgacatggca atggagcggc gaatcgattt
      481 gcctgcatta tttaagacaa tatcaacatg gccatgttct gccaatattt gttcggcgac
      541 ggcttttgca tgttcaggtt tactcaggtc agttggataa ataaacgccc tgccacctgc
      601 cttattaata tcgtcagcga cggcttgaag tttatcggca ctacgtgcca ctaataacac
      661 gatcgcccct gcttttgcta aatgtttagc ggtttcttca cctataccaa acgatgcgcc
      721 tgtaattaaa ataagtttgt tattaacagc atcatgtagt ttttgtggtt tatctagccc
      781 acgtggattg agtaattttg aggccaaacg aatgatgagt ggttgttttg gattattcat
      841 ggtgtcattc cttttttaag gttatgtttg acaaaaaact atgcttattt aagccacaaa
      901 gcattgacca accaaatagt gattttaggc caaaaattga gttcccctcg ccctttggga
      961 gtgagtaata taattatatt tattacgtta aatggctttt taa
//
LOCUS       DAOQMB010000928         1003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_150767_length_1003_cov_11.931435, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000928 DAOQMB010000000
VERSION     DAOQMB010000928.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150767_length_1003_cov_11.931435"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLY05_15070"
     CDS             complement(<1..190)
                     /locus_tag="PLY05_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004650779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HNC04662.1"
                     /translation="MSQQDFGRVAVLFGGTSAEREISLKSGQAVLQALQQAGVNAQAF
                     DPAEQNIGDIKAYDRAFIV"
     gene            complement(195..>1003)
                     /locus_tag="PLY05_15075"
     CDS             complement(195..>1003)
                     /locus_tag="PLY05_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003654096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HNC04663.1"
                     /translation="KDTFIEFLHNLPFYGLAVVCGDDAVIRELLPRIARPTVTYGFAE
                     TNDVRAVDVRQEGMQTHFTALRPQKEPLAISLNLPGLHNVLNALAAIVIATDEGVEDA
                     AIQRALVNFQGVGRRFQQYGEFEFGQGSVRLVDDYGHHPREVEATLKAARQSFPDRRL
                     VLMFQPHRYTRTRDCFEDFVQVLSQVDVLLLLDVYSAGEKPIAGADGRTLARSIRARG
                     LVEPIFIEKTEELNTLLKTVLQPNDVLLTQGAGTVGALAIELAQNNLYLG"
BASE COUNT          313 a          247 c          184 g          259 t
ORIGIN      
        1 acacaataaa cgcacgatca taggctttaa tatcaccaat attttgttcg gcagggtcaa
       61 acgcttgagc attaacgcct gcttgctgta aggcttgcaa cacggcctga ccacttttta
      121 aagaaatttc acgttctgcg gatgtgccac caaacaatac ggcaacacga ccaaaatctt
      181 gctgactcat aaactcaacc taaatataaa ttattttgtg ctaattcaat ggctaacgca
      241 ccgactgtac ctgcaccttg agttaacaac acatcattag gctgcaacac cgtttttaat
      301 aaggtattca actcttcggt tttttcaata aaaatcggct caaccaaacc acgcgcacga
      361 atactccgtg ctaaggtgcg tccatctgca cctgcaatcg gcttttcacc cgctgaatac
      421 acatccaaca ataataaaac atcaacttgc gataacacct gtacaaaatc ttcaaagcaa
      481 tcacgggtac gggtgtaacg atggggttga aacattaaca ccaaacggcg atcgggaaag
      541 ctttgtcgtg cggcttttaa ggtggcttca acttctcgtg ggtgatgacc ataatcatcc
      601 actaaacgca ccgagccctg accaaattca aattcgccat attgttgaaa acgacgaccc
      661 acaccttgga agtttactaa cgcacgttga atagctgcgt cttctacgcc ttcatccgtg
      721 gcaatcacaa ttgctgctaa ggcattcaaa acattgtgta aaccgggtaa atttaggcta
      781 attgctaatg gctctttttg tggacgcaag gcagtaaaat gggtttgcat accttcttgg
      841 cgaacatcga cagcacgcac atcattagtt tcagcaaaac cataagtaac cgttggccgc
      901 gcaattcttg gcaataattc acgaatcacg gcatcatcgc cacaaactac tgctaaacca
      961 taaaatggta agttgtgtaa aaactcaata aaagtatctt tga
//
LOCUS       DAOQMB010000929         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_151083_length_1002_cov_7.159451, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000929 DAOQMB010000000
VERSION     DAOQMB010000929.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151083_length_1002_cov_7.159451"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLY05_15080"
     CDS             complement(<1..316)
                     /locus_tag="PLY05_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNC04664.1"
                     /translation="MNLHEYQGKQLFAEYGLPVSKGFAASTPEEARQAAEQIGGDVWV
                     VKAQVHAGGRGKAGGVKLVKTLDEVEQFAREKLGTNLVTYQTDSKGQPVSKILVETCT
                     DIA"
     gene            complement(488..>1002)
                     /locus_tag="PLY05_15085"
     CDS             complement(488..>1002)
                     /locus_tag="PLY05_15085"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996415.1"
                     /note="E3 component of 2-oxoglutarate dehydrogenase
                     complex; catalyzes the oxidation of dihydrolipoamide to
                     lipoamide; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HNC04665.1"
                     /translation="TNVEGVYAIGDLVRGPMLAHKAMEEGVMVAELIAGHYAQINYDT
                     IIGVIYTHPEIAWVGKTEEQAVAAGIEVKTGQFPFQVNGRALAANDAQGLVRFVADAK
                     TDRLLGMAVIGSNAGDMVHQGMIALEFAASIEDLQLMTFAHPTLSEAVHEAALSVDGR
                     AIHAIQRKRK"
BASE COUNT          285 a          238 c          182 g          297 t
ORIGIN      
        1 tagcgatatc agtacaagtt tcaaccaaaa tcttgctaac aggttgacct ttgctgtctg
       61 tttgataggt cactaaattt gtacctaatt tttcacgggc gaattgttca acttcgtcta
      121 aggttttgac gagctttaca ccaccagctt taccacggcc accagcatga acttgagctt
      181 taacaaccca aacatcacca ccgatttgtt cagcagcttg acgggcttct tctggcgtgg
      241 atgcggcaaa cccttttgat acaggtaaac cgtattcagc aaacaactgt ttgccttggt
      301 attcatgtaa attcattctt tcaccctgtt aatttgctca ggacttaaac aacaaactaa
      361 gtcctatggt taagagcagc ttatgctcat gacgcttgtt tatccgctgt taacggaata
      421 ctaatttagg ttatcctcac actaaatttt cttttaaaga gtttaggtga ggataaaatc
      481 aaaacaatta tttacgctta cgctgaattg catgaatcgc tctgccatca acagataaag
      541 cggcttcatg tacggcttca gacaatgtag gatgcgcaaa agtcattaat tgcaaatctt
      601 cgatgcttgc agcaaactct aaagcgatca tgccttgatg caccatatca cctgcatttg
      661 aaccaatgac agccattcct aacaaacggt ctgttttagc atcagcaaca aaacgtacca
      721 aaccttgtgc atcattggca gctaatgcac gaccattcac ttggaatggg aattgaccag
      781 tttttacttc aatgcctgca gcaacggctt gttcttcggt tttgccaacc catgcaatct
      841 ctggatgggt ataaatgacg ccaatgattg tgtcgtagtt aatttgtgca taatgacctg
      901 caattaactc tgcaaccatc acgccctctt ccatggcttt atgagccaac attgggccac
      961 ggactaagtc accaatcgca taaacgcctt ctacattagt ac
//
LOCUS       DAOQMB010000930         1002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Agitococcus sp. isolate AMR_MDS_0533
            NODE_151084_length_1002_cov_6.998944, whole genome shotgun
            sequence.
ACCESSION   DAOQMB010000930 DAOQMB010000000
VERSION     DAOQMB010000930.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810165
            Sequence Read Archive: SRR3501856
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Agitococcus sp. (wastewater metagenome)
  ORGANISM  Agitococcus sp.
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Agitococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 06:24:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,018
            CDSs (total)                      :: 2,986
            Genes (coding)                    :: 2,952
            CDSs (with protein)               :: 2,952
            Genes (RNA)                       :: 32
            tRNAs                             :: 29
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 34
            CDSs (without protein)            :: 34
            Pseudo Genes (ambiguous residues) :: 0 of 34
            Pseudo Genes (frameshifted)       :: 5 of 34
            Pseudo Genes (incomplete)         :: 29 of 34
            Pseudo Genes (internal stop)      :: 2 of 34
            Pseudo Genes (multiple problems)  :: 2 of 34
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Agitococcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_151084_length_1002_cov_6.998944"
                     /isolate="AMR_MDS_0533"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:1872415"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-07-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..509)
                     /locus_tag="PLY05_15090"
     CDS             complement(258..509)
                     /locus_tag="PLY05_15090"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNC04666.1"
                     /translation="MESNDNEKIAFKRYIACLLMLLVLGASNQVLAMLSDAQAVNKAD
                     LQRMLSQRIAKLYLAMYWAIPYKNLETELNQAMDNYEQG"
BASE COUNT          312 a          202 c          157 g          331 t
ORIGIN      
        1 gcaaccgtca aagaggctct aaagccgact gtgtttagcc aatttgggac tgtcaccact
       61 taaattaaat actctgagtt cggtttaaaa attctagttt gtagagggaa gtcccttttt
      121 gagagtaatg ccagttagtt aagcttgttt gctgacttgg tttcgactca accaccctga
      181 cgccgctcag tcaacgcaaa catgcgttct gaacggagtc aaagggttat ttttaagaag
      241 ccattttccg taaccgacta gccttgttca taattatcca tcgcctgatt taattcggtt
      301 tctaagtttt tgtaaggaat cgcccaatac atagccaaat acaacttagc aatacgttga
      361 cttaacatcc gttgtaaatc tgctttgttc actgcttgag catcacttaa catcgccaag
      421 acttgattac ttgcacctaa aactaacaac attagcaggc aagcaatata tcgtttaaac
      481 gctatttttt cattatcatt agattccatg taggacaact gattatattg ttattgtcct
      541 ttattgttat ttattagcaa caagacgctg ctatcctttt tgagttttaa aataaccgtc
      601 attagctgtc ttaaaatcag atttttgcag caaaaacaat atcatatatt gcttttacca
      661 aacaaaatta tactatgatt taggcaaatt tttgttcaaa atacaagcag aatcgttgaa
      721 tacagaacta catccttgtt tattaaagcg ataggaaagt ttgtttatct ttttaaatac
      781 aactctgatt tgttgcttac ctcattgtaa acaagatagc tactcagagt cccataacat
      841 agcggattgc tttatgataa aaatgctcac caagtaggcg acaaacggtt atataattct
      901 gaccaagtgt tagggcttaa aaaaccctaa caccttaaaa ataatctaca atcttgctgc
      961 caattctgcc ccctctttaa tcgcacgttt agcatctaat tc
//
